LOCUS       DASRJQ010000001         3158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3663135, whole genome shotgun sequence.
ACCESSION   DASRJQ010000001 DASRJQ010000000
VERSION     DASRJQ010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3663135"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..787)
                     /locus_tag="VFB89_00005"
     CDS             complement(200..787)
                     /locus_tag="VFB89_00005"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZM25725.1"
                     /translation="MTAVPHSDTRIRVHPERSVPEQALEILVAGLVAHVGIVEDGQPV
                     VIPMTYQFDPAEPDVLYLHGGPSSRLLSHLATGAPVCVTVTLLDGLVYSKTALYHSVN
                     YRSVVCFGRGAPTPAPERAREILHAMVLRCFPDRVIGRDFAPIPDTHLDATALVPLRI
                     DAMSAKARRGGPKGPGDNDPTKPGTAGVIPLKATP"
     gene            876..2360
                     /locus_tag="VFB89_00010"
     CDS             876..2360
                     /locus_tag="VFB89_00010"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HZM25726.1"
                     /translation="MPSRPTPLTLVPIDPEDDVPLHRQLYDGFRDAILTGRLPPGGRI
                     PSSRLLARELGLSRNTVALAFAQLHAEGYIEGQLRSGTYVSRTVPDALLRAQWRPADR
                     ATASSGRAGLSARGRALAGTGLHSEEPAPKPPRAFRPGLPALDAFPRRLWTRLANRRW
                     RGGHDLPLAYSDPAGFRPLREAIAGHVAAARGAHCTAEQVLVVSGSQQALDLAARVLL
                     DPGDAVWLEEPGYLGARAALLGAAARIVPVPLDREGLSVTEGERDAPSARMVCVTPSH
                     QYPTGVTMSAGRRLSLLQWAARADAWIVEDDYDSDFRYASRPLACLQGMDAGGRVVYI
                     GTFSKTLFPALRLGYLIVPLHVADAFRAARAVCDRHSPTIDQAVLADFIGEGHFGRHV
                     RRMRTLYEERQVALVEAGRAVEGLIEIRPAEAGLHLVGWLPPGVSDRRAAAALKQEGI
                     EALALSPHYIGSTEREALLLGYAAFDRRAIRRGVEKMGAVLRRL"
     gene            complement(2395..>3158)
                     /locus_tag="VFB89_00015"
     CDS             complement(2395..>3158)
                     /locus_tag="VFB89_00015"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HZM25727.1"
                     /translation="DYSKLVLVPGPYVTNYLAAQRFETDRQLAQVGKPVDRTEWRMTP
                     PTYNAYYNPAFNEIVFPAGILQPPLFDPTADDAVNYGATGATIGHELTHGFDDQGRYF
                     DAVGNLRNWWTTQDSAQYEKRAQLVVDQYNGYIAVDTFHVNGRLTLGENIADIAGLTI
                     AYDAWRRSLAGKPEPAPIDGFTAKQRFFIAYAVSWREKVRPEMERTWAVSNPHSSVRW
                     RVNGVVGHVPAFAEAFGCKAGDSLARAPEQRMQIW"
BASE COUNT          511 a         1032 c         1032 g          583 t
ORIGIN      
        1 cctgacaagg cgatcgatca gctcgaggct ttgctcaagg taccttacaa cctctcacca
       61 ggtctgcttc ggatcgatcc caatttcgca ccgcttcgga agaaccctcg ctttcagcgc
      121 ctgatcgctg ctcagtaacc gccaggtccg gcaccgctgc tcgaaaaacg tggcgcggct
      181 cacgggctga tcccgcaatt cacggcgtcg ccttaagcgg aatcacgccg gcagtgcctg
      241 gtttggtcgg atcgttgtcg cccggcccct tcggaccacc ccgacgtgcc ttggcactca
      301 tggcatcaat tcggagcggc acgagcgccg tggcgtcgag gtgtgtatcg ggaatcgggg
      361 cgaagtcgcg cccgatgacg cggtcaggga agcagcgcaa aaccatggcg tgaaggatct
      421 cacgagcccg ctcgggcgca ggcgtcgggg ccccgcgccc gaagcacacc acgctcctgt
      481 agttcaccga gtggtacagc gcagtcttcg agtagaccaa tccgtccagc agcgtcaccg
      541 tgacgcacac cggcgctccg gtcgcaagat gagagaggag ccggctgctc ggccctccgt
      601 gcaggtataa cacgtccggc tcggcggggt cgaactgata agtcatcggg atgacgaccg
      661 gctgaccgtc ttcgacgatc cccacatggg cgactaaccc ggcaacgaga atctcgagtg
      721 cttgctcggg caccgatcgc tcggggtgaa cccggatgcg ggtgtcggag tggggaacgg
      781 ccgtcatggc gatatattag gtcgaaactg gccctgttaa aagagccaga tcgtgccgag
      841 ctatggtgcc agataccgcc gcccgagttc gtcccatgcc cagccgtccc acgcccctca
      901 cgctcgtccc catcgatccg gaagacgacg ttccgcttca ccgccaactg tacgacggat
      961 tccgggatgc gatcctgacc gggcggctgc cgcctggggg aaggattcca tcctcgcggc
     1021 tcctggcccg ggagctgggc ctgtcgcgca acacggtcgc ccttgccttc gcgcaactcc
     1081 acgcggaggg gtatatcgaa ggccagctgc gctcgggaac ctatgtctcg cgaacggttc
     1141 cggatgcgct gctgcgcgcg cagtggcgac cggctgatcg ggcaacggca tcatcgggtc
     1201 gggccggtct gtcggctcgg ggcagggcgc tcgccggcac aggcctccat tcggaagagc
     1261 ccgctcccaa accgccgaga gctttccgtc ccggcttacc ggcactggac gcgtttcccc
     1321 ggcggctctg gactcgtttg gcgaaccgcc gctggcgcgg cggccacgac ctgcccctcg
     1381 cttacagcga tccagcaggc tttcgcccgc tcagggaagc catagccggg catgtcgccg
     1441 cggcgcgagg cgcgcactgc accgccgagc aggtgctcgt ggtgagtggc tcgcagcagg
     1501 ccctggatct ggctgcgcgg gtgctgctcg acccgggtga cgccgtgtgg cttgaggagc
     1561 ccggctatct cggtgctcgg gcggcgttgc tcggcgcagc ggcgcgcatc gtcccggtcc
     1621 cgctcgatcg cgaagggttg agcgtcaccg agggcgagcg ggacgcgcct tcagcccgaa
     1681 tggtctgcgt cactccatcg catcagtacc caaccggagt caccatgagt gcgggacgcc
     1741 ggctttcgct gctgcagtgg gctgcgcgcg cagatgcctg gatagtggag gacgattacg
     1801 acagcgattt tcgctacgcg agccggccgc tcgcctgcct gcagggtatg gacgccgggg
     1861 ggcgcgtggt ctacatcggc accttcagca agacgctttt ccccgccctc cggctcggct
     1921 acctgatcgt tcccctacat gtggcggatg catttcgtgc ggctcgcgcg gtgtgcgatc
     1981 ggcactctcc caccatcgat caggcggtgc ttgccgattt catcggcgag ggccacttcg
     2041 ggcgccatgt tcgcagaatg cgcacgctgt acgaagagcg gcaggttgcg ctggtagaag
     2101 cgggacgtgc cgtcgagggc ctaatcgaga ttcggcccgc cgaagccgga ttgcacctgg
     2161 tcgggtggtt gccgcctggt gtgagcgacc gtcgagctgc tgctgcgctc aagcaggagg
     2221 gcatcgaggc ccttgcactc tcaccgcact acatcggatc gacggagcga gaggcacttc
     2281 tcctgggcta tgcggcattc gatcggcggg cgatccggcg gggagtggaa aagatgggag
     2341 cggtgctccg gcgactctga catcgtccac cgctgtctcg gtgtagcctg gaccttacca
     2401 gatctgcatt cgctgctccg gcgcccgagc cagggaatca cctgccttgc agccgaacgc
     2461 ctcggcgaac gctgggacgt gccctaccac gccgttcact ctccagcgca ccgagctgtg
     2521 cgggttggag actgcccacg tgcgctccat ctcgggccgc accttttcgc gccatgacac
     2581 ggcatacgcg atgaagaacc gctgctttgc tgtgaagccg tcgatgggtg ctggctccgg
     2641 cttgccggcg agtgaccttc tccaggcgtc gtacgcgatg gtcagcccgg cgatgtcggc
     2701 gatgttttcg cccagcgtga gcctgccgtt cacatggaac gtgtcgaccg cgatgtatcc
     2761 gttgtattgg tccactacca gttgcgcacg cttctcgtac tgcgccgaat cctgcgttgt
     2821 ccaccagttc cgaaggttac cgactgcgtc aaagtagcgg ccctggtcgt cgaatccatg
     2881 agtaagctcg tgaccaatcg tggcgccggt ggcgccgtag ttcacggcgt cgtctgcggt
     2941 gggatcgaac aagggtggct gcaggatgcc ggccgggaaa acaatttcat tgaacgcggg
     3001 attgtagtag gcgttatacg tcggcggcgt catcctccac tcggtccggt ccaccggctt
     3061 gcccacctgc gccagctgtc ggtcggtctc gaaccgctgc gctgccagat agttggtgac
     3121 gtacggcccg gggaccagca cgagcttcga gtagtccc
//
LOCUS       DASRJQ010000002         6070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1970780, whole genome shotgun sequence.
ACCESSION   DASRJQ010000002 DASRJQ010000000
VERSION     DASRJQ010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6070
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1970780"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VFB89_00020"
     CDS             <1..608
                     /locus_tag="VFB89_00020"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HZM25728.1"
                     /translation="LKEMTPVAVRDRLQARSTLIVPVGTTEQHGPHLPLGCDTMIVER
                     LADDLSSASGIPRAPAVEYGVQPPTHALPGGAALRRKTLHRVMNELIEAWEEGAGVRE
                     FVILTAQANDAHLEALSTIRTGTASVVLLDVFGLDFGSLLSSPEPLAHGGELDTSLLL
                     YIAPELVTVELLTTASLASATKGEGLYKFILDRLTQRLFNP"
     gene            626..1279
                     /locus_tag="VFB89_00025"
     CDS             626..1279
                     /locus_tag="VFB89_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25729.1"
                     /translation="MRAWVAPWLLMWASALSAQQAYENALTIEQGGKEIGREEYSIQR
                     GSGQSATVIKGYARYPTTSPKLQIALTLERSPTAGIAKFELDVQAPEGNVVILAAGSG
                     ARLIVRSVTKGSEAGRELPGGKDLVLLDENMYSLYLQLVDAATPSGARLTAIYPRTGR
                     RATLVARRDAGSNGTVQVQMTGEVSGMLTVDAQGRLQRLELSGSGIVVSPTAPPAKK"
     gene            1377..2903
                     /locus_tag="VFB89_00030"
     CDS             1377..2903
                     /locus_tag="VFB89_00030"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZM25730.1"
                     /translation="MRRWLVVPFLVAAAPTATAQQPQDTFAVRPRPTAGLSLADALGQ
                     GRAKSPAYLQVVNNADPARWGVRNAYATFLPTVSASGGLGYTGSGQSQFGAFFGPTSP
                     FVSSNYQIGLAWRFDGRVLAGPSQQKALQRATDEDIEDAGVGLKADITTQYLNALAAV
                     AQTEVAREQVKRNNEFLRLARARNQVGQATLLDVKQAEATQVSSEVALLQAYQAENDA
                     KLELFRRMGVAPPVPIQQIELTDSFPVVAPSYSRDELLKLAEEQNPSLKALRERETAA
                     RANVRAVRSDFFPRLTAQAGWSGFTQQFTNENLLLQQQLATGQQAAAQCTFDNQVRAG
                     LNLGGIVPDCFGPNGLDPTGTMLNATTTQQVHNGNNVFPFSFTGQPFQAALTISLPIF
                     TGFSRHLQLAEARAQQKDADENVRAQALLIRANVESRYLALGATYQAIGAQATGRDAA
                     REQLRLAQDRYRVGLGSALEVSDAQNAVQKAEGDYISAVYAYHRAITALEAAVGRPLR
                     "
     gene            2916..4205
                     /locus_tag="VFB89_00035"
     CDS             2916..4205
                     /locus_tag="VFB89_00035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZM25731.1"
                     /translation="MSRGVKIGLIIGTVLLVAGGGIGAAVAKKKHKAVEVRMDSVAHR
                     DLVAAVTASGKIDAEKTVDISADITGRILQIAVKEGDTVRKGQFLVQIDPVQFQGAVN
                     RGEALLAANEAAVVQARTNRDQTKRQLDRAIEIRRTAPELIAEEAVEQAQQAYDVAVS
                     TYTANVAQVEQARASLKEARDNLTRTRLFSPISGRVVRLPVHEGEVAVPNTFSKETAL
                     LMTIADLSTILAKVQVDETDVVRLHMGDSVDVSIDAYPDTTFLGRVTKISNAAKLTQT
                     TAGANTGTNDRAVDFDVEITLTNPPKDIRPDLSCTARIVTDMRKNVLSVPIIALTVRE
                     HEKLPNEVTSPQDTIRAAKKFRKKETEGVFVVRDGIATFRPVKIGIAGDEYFEVVSGI
                     RDGETIVGGTYQAIRDLKDGDRVKQAPEPKKGETKTL"
     gene            4202..4903
                     /locus_tag="VFB89_00040"
     CDS             4202..4903
                     /locus_tag="VFB89_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZM25732.1"
                     /translation="MSDAIIKIRNLTRLYDMGGELVHALRGVSLDVARNEYVAIMGPS
                     GSGKSTMMNLLGCLDTPSSGEYWLNGEEVSRLSDNALARVRNREIGFVFQTFNLLPRA
                     TALHNVELPLVYAGLSAGERRERARHALDQVGLSNRKDHRPNELSGGQRQRVAIARAL
                     VNQPSILLADEPTGNLDSATSEEIMRVFGDLHAAGQTVVMVTHEADIAANAARVITLR
                     DGRIESDRPTRDRAA"
     gene            4914..>6070
                     /locus_tag="VFB89_00045"
     CDS             4914..>6070
                     /locus_tag="VFB89_00045"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM25733.1"
                     /translation="MPIFEAVRLALRTIRVQKLKSFFSLIGVLIGVTFLIAVVSIVQG
                     MNHYMQDRFANGLIGVNTFEVRRFPNYDVGNVPEDTWREYLRRPRVNYQEAAFLRERV
                     HTPTIYARTCFEDLWVNQGTLRGQIEVRAADEEYFRIKNYRIAEGRTFTPQEARSGLA
                     VIVIGDLVREKFFPGVDPIDKELTIAGLPYRVVGVVEKQGTLFGLSLDRFGIVPFLAP
                     ARRELCAENVVHELWAKSAGPTEMLAAVGETEQYMRIARKLEPDQANNFYIETAEGAL
                     GTWAKISRILFLALPGLVAISLVVGGIVIMNIMLMAVSERTREIGIRKALGARRRDIL
                     AQFVVEAATLSSAGAVLGIGTGLVLAFVVKALTPLPAAVAPWSIGLAVFLGV"
BASE COUNT         1162 a         1944 c         1921 g         1043 t
ORIGIN      
        1 gattgaagga gatgactccg gtagcggtac gggatcggct ccaggccaga tccacgctga
       61 tcgtgccggt aggaacgacc gagcagcacg gtccgcacct tccgctcggg tgcgacacca
      121 tgatcgtcga gcgtctggca gatgacctct cgagcgcgtc cgggatcccg cgggcacccg
      181 ccgtcgagta cggagtccag ccgccaaccc atgccctgcc cggcggtgcg gccctgcggc
      241 gcaagacgct acatcgggtc atgaacgaac ttatagaagc ctgggaggag ggggcaggcg
      301 tccgcgagtt cgtgattctg acagcccagg cgaacgacgc acacctggag gcattgagca
      361 cgatccgaac cggtacggcg tcggtagtac tcctcgacgt tttcggtctc gatttcggct
      421 cgctcctttc ctctcccgag ccgctggccc atggcggaga actcgatacc tccctgctcc
      481 tctatatcgc ccccgagctg gtaacggtcg agctgctcac cacggcgtca ctcgcgtcgg
      541 cgacgaaggg cgaaggcctg tataaattca ttctcgatcg ccttacccaa cggctcttca
      601 acccgtgatc ggagcgtcga cgttcatgcg tgcgtgggtc gctccctggc tgctgatgtg
      661 ggcctcagcc ctgtcggctc agcaggcgta cgagaacgcc ctgaccatcg agcagggagg
      721 gaaagagatc ggccgcgagg agtactccat tcagcgcggc tccggccaga gcgctaccgt
      781 catcaagggg tacgcgcgat acccgacaac gtcgcccaag ctacagatcg ccctgaccct
      841 ggaacgttcg ccgacggcag gcatcgccaa gttcgaactc gacgtgcagg cgccggaggg
      901 taacgtcgtc attttggcgg caggatccgg cgcacgactg atcgttcgct cggtgaccaa
      961 aggatcggag gcgggacgcg agctgccggg ggggaaggac ctcgtattgt tggacgagaa
     1021 catgtactcg ctttacctcc agctggtcga tgccgcgact ccttcaggag ccaggctcac
     1081 ggcgatctac ccgcggaccg gccggagagc gaccctggtc gcgcgtcgag acgcggggtc
     1141 gaatggcacg gtccaggtgc agatgaccgg cgaggtgagc ggcatgctga ccgtggacgc
     1201 tcaaggccgg ctgcagcgac tggagctatc agggagcggc attgtggtgt caccgacggc
     1261 acccccggca aagaagtgag cccgtcggcc ctgccagcct ttgtcgcaac ctgaaactta
     1321 tcgtcacatc ccgccgtagg tgggccgttc tcgcttacca cgtaaaggac accgacatgc
     1381 gccgttggct tgttgtgccc ttcctggtcg ccgccgcccc gaccgcgacc gcccaacaac
     1441 cccaggacac cttcgccgta cgaccccggc cgaccgcagg cttgagcctg gccgatgccc
     1501 ttggccaggg gcgagccaag agtccggcat acctccaggt ggtcaacaat gccgacccgg
     1561 cgaggtgggg agttcggaac gcgtatgcca cgttcctgcc caccgttagc gcatcgggcg
     1621 gactcggata caccggctcc gggcagtccc aattcggtgc gttcttcggg ccgaccagcc
     1681 cgttcgtaag ctcgaactat cagatcggtc tggcatggcg tttcgatggc cgagtcctgg
     1741 ccgggcccag ccaacagaag gctctgcaac gagccaccga cgaggacatc gaggatgcgg
     1801 gcgtaggcct caaggccgac atcacgaccc agtacctcaa cgccctggca gcggtcgccc
     1861 agaccgaggt cgcccgcgaa caggtcaagc ggaacaacga gtttctccgc ctcgcgagag
     1921 cccggaatca ggtgggtcaa gcgacgctgc tcgatgtgaa gcaggccgaa gcgacgcagg
     1981 tcagctccga agtagccctg ctgcaagcat atcaggccga gaacgatgcc aagctcgagt
     2041 tgttccggcg gatgggggtc gcaccacccg tcccgattca gcagatcgag ctcaccgact
     2101 ccttccccgt cgtggcgccc tcctatagtc gcgacgagtt gctgaagctg gcggaggaac
     2161 agaacccgtc tctcaaggcg ctcagggaac gcgagacggc cgcccgcgcg aatgttcggg
     2221 ccgtacggtc ggatttcttc ccccggctca ccgcgcaagc cgggtggtcc ggctttaccc
     2281 agcagttcac caatgagaac ctgttgcttc agcagcagct ggcgacgggg cagcaggcgg
     2341 cggctcaatg cacctttgac aaccaggttc gtgcagggct caacctgggc ggcatcgtgc
     2401 ctgactgttt cggcccgaat gggctggatc ctacgggcac gatgctgaac gcgactacaa
     2461 cgcagcaggt ccacaatgga aataacgtct ttccattcag cttcaccggc cagccatttc
     2521 aggccgcgct cacgatttcg ctcccgatct tcaccggatt cagccgccac ctgcaactgg
     2581 ccgaggcccg ggcccagcaa aaggacgcgg acgagaacgt gcgagcccag gccctcctga
     2641 ttcgtgccaa cgtcgagagc cgctatctcg cgcttggagc tacctatcag gcgatcggcg
     2701 ctcaggccac gggtcgggac gctgcccggg agcagctgcg cctagcccag gaccgttacc
     2761 gggttggcct cggctccgca ctcgaggtat cggacgcgca gaacgccgtc cagaaggccg
     2821 aaggcgatta catctcggcg gtctacgctt atcaccgcgc catcaccgcg ctcgaggccg
     2881 cggtcggccg tccccttcgt tagcgaggaa cgagcatgtc tcgtggagtg aagatcggtc
     2941 tcatcatcgg cacggtcctc ctagtcgcgg gcggagggat cggcgccgcc gtcgccaaga
     3001 aaaaacacaa agcggtcgag gtccgtatgg attccgtggc gcatcgggac ctggtggcag
     3061 cggtcacggc cagcgggaag atcgatgcgg agaaaacggt agacatcagt gccgacatca
     3121 ccggccggat cctgcagatc gccgtgaaag agggcgacac ggttcgaaag gggcagtttc
     3181 tggtgcagat cgatccggtc cagttccagg gtgccgtgaa ccgtggtgag gccctgctcg
     3241 ccgccaacga agcagccgtc gtgcaggccc gcaccaacag agatcagacg aagcgccaac
     3301 tcgatcgggc catcgagatc cgccgcaccg cgccggagct gatcgccgag gaggccgtgg
     3361 agcaggctca acaggcttat gacgttgcag tgtccaccta caccgccaac gtggctcagg
     3421 tcgagcaggc ccgggccagc ctcaaggagg cgcgagataa tctgacccgt actcgcctct
     3481 tctctcccat atcgggtcgg gtggtgcgcc tgccggtcca cgagggtgag gtcgcggtgc
     3541 ccaatacgtt ctcgaaggag acggcattac tcatgaccat cgccgacctc tctaccattc
     3601 tggccaaggt gcaggtggac gagaccgacg tggtccggct gcacatgggc gattcggtgg
     3661 acgtgagcat cgatgcctat cccgacacga ctttcctcgg tcgggtcacc aagatcagca
     3721 acgccgccaa gctcacccag acgaccgcag gagccaatac cgggaccaac gatcgtgcgg
     3781 tcgacttcga tgtcgagatc acgctgacca atccgcccaa ggacatccgc ccggacctga
     3841 gttgcacagc gcgcatcgtg accgacatgc ggaagaacgt tctgtcggtg cctattatcg
     3901 cgctcaccgt gcgggagcac gagaaactgc ccaacgaggt cacctctccg caggatacga
     3961 ttcgggccgc caagaaattc cggaagaaag agaccgaagg cgttttcgtc gtacgtgatg
     4021 gaatcgccac cttccggccg gtcaagatcg gcattgccgg agatgagtac ttcgaggtcg
     4081 tgagcggtat ccgcgatggg gagacgatcg tcggcgggac gtatcaggcc attcgcgacc
     4141 tcaaggacgg tgatcgggtg aagcaggcac cggagccgaa gaaaggcgag accaagacgt
     4201 tatgagcgac gcgatcatca agattcgcaa ccttacccgg ctctacgaca tgggcggaga
     4261 gctggtccac gcgctgcggg gcgtaagcct cgacgtcgcc cggaacgaat acgtggcgat
     4321 catgggaccg tcgggctccg gcaagtccac catgatgaac ctgctcggct gcctcgacac
     4381 tccgagctcc ggcgagtact ggctgaatgg tgaggaagtc tctcgcctga gcgacaacgc
     4441 tctcgcgcgg gttcgcaatc gggagattgg cttcgtcttt cagactttca atctcctgcc
     4501 ccgtgcaacc gcactgcaca acgtcgagct gccgctcgtg tatgccggcc tgtccgccgg
     4561 cgagcgacga gagcgcgcac ggcacgcgct cgatcaggtc ggcctctcca atcggaaaga
     4621 ccatcgtccc aacgagctgt cgggcggaca acggcaacgg gtggcgatcg ctcgcgccct
     4681 ggtgaatcaa ccttccattc tgctggccga tgagcccacc ggaaacctgg atagcgccac
     4741 cagcgaagag atcatgcggg tgttcggcga tcttcacgcc gccggccaga ccgtggtgat
     4801 ggtcactcat gaggccgaca ttgcggccaa cgcggcccgc gtgatcaccc tgcgtgacgg
     4861 ccgaatcgag agcgaccggc cgacccggga ccgcgccgca tgacgggacg gtaatgccga
     4921 tctttgaggc ggtgcggctg gcgctacgga ccatccgggt gcagaagctc aagagcttct
     4981 tctccctgat cggggtgttg atcggcgtca ccttcctgat tgcggtcgta tcgatcgtgc
     5041 aggggatgaa tcactacatg caggaccgct ttgccaacgg gctgatcggg gtgaatacgt
     5101 tcgaggtccg gcgctttccc aactacgacg tcgggaacgt tcccgaggat acctggcggg
     5161 aatatctccg ccgcccccgc gtgaactacc aggaggccgc atttctgcgg gagcgggtcc
     5221 atacgcccac catttacgcg cgcacctgct tcgaagacct gtgggtgaac cagggaaccc
     5281 tgcgcgggca gatcgaagtt cgcgcagccg acgaggaata cttccggatc aagaactacc
     5341 gaatcgctga gggccgcacc ttcactccgc aggaggctcg ctcgggcctg gcggtcattg
     5401 tgattggtga cctggtccgg gagaagtttt tccccggcgt cgacccgatc gacaaggaac
     5461 tcaccattgc cggcttgccg taccgggtcg tgggcgtggt cgagaagcag ggcacgctct
     5521 tcgggctttc tctggatcgt ttcggaatcg tgccctttct cgccccggcg cgacgggagc
     5581 tctgcgcaga aaacgtggtg cacgaactct gggccaagag cgccggacct actgaaatgc
     5641 tcgcggcggt aggcgagacc gagcagtaca tgcggatcgc ccggaagctc gaacctgacc
     5701 aagccaacaa cttctatatc gagaccgctg aaggcgcctt gggaacctgg gccaagattt
     5761 cccgaatcct gtttctggcc ttgcctggtc tggtcgccat ctcgctcgtg gtcggcggca
     5821 tcgtgatcat gaacatcatg ctgatggccg tgagcgagcg tacccgcgag atcggaattc
     5881 gtaaagcctt gggtgcccgc cggcgcgaca tcctggccca gttcgtcgtc gaagcggcca
     5941 cgctctcgtc tgcgggggcc gtcctcggca tcggaaccgg tctggtgctg gccttcgtgg
     6001 tcaaggcgct cacaccacta cccgcggccg tggcgccctg gtcgatcgga ctcgccgtgt
     6061 tcctgggcgt
//
LOCUS       DASRJQ010000003        10702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1970650, whole genome shotgun sequence.
ACCESSION   DASRJQ010000003 DASRJQ010000000
VERSION     DASRJQ010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 10702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10702
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1970650"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VFB89_00050"
     CDS             complement(<1..524)
                     /locus_tag="VFB89_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25734.1"
                     /translation="MPRNVLPRLGASSRIAVLALATLLSGTLTLSAQQTDSATTSRDS
                     LEERLRRAEEAIELLRRQLETQAASEVRTKSRVQFEFFGRVLVNTFYNSGRGNVVDVP
                     QFVLTDAGAPTRDGSFSAAVRQSLVGGAIHISSVLGGRFTGDLALDLFGATQSNPSRT
                     LPVVRLRTARAILQ"
     gene            523..1212
                     /locus_tag="VFB89_00055"
     CDS             523..1212
                     /locus_tag="VFB89_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25735.1"
                     /translation="MRQARSKIAAVVLWAMLAPGALTAQGSVSGRVTIQEREGEATKD
                     LATAVIYLETSGSAIGPARDAEAEIAMHDKTFIPHVQVVPAGASVAFPNQDPFRHNVF
                     SNSSAGPFDLGLTPRGQSSKNQFRKSGVYAIYCNIHSRMSAFVVAVASPYYTQPGADG
                     SFTISAVPAGRYVLHAWHERAGETTQTLEVPAAGLQSLAAVLDARGYRPAQHKNKFGQ
                     DYAASDDDERY"
     gene            1190..3259
                     /locus_tag="VFB89_00060"
     CDS             1190..3259
                     /locus_tag="VFB89_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM25736.1"
                     /translation="MTTTSGTSGLTWRIFFGAAGVVAIVLLGTLLLAWASVRRTAGTE
                     LDRSLARTRELVVTFLQGDRRSLSQSAAVFVQNPNFRALLENPAPGDMLDQSLEAAHL
                     LGATFVQITDASGVRLARSDEPQAALVQLARSPLIAGALEGDMRAGFGVSGDSILFQA
                     VAVPIPGAGPQVVRGSLMAAKAIDSTLASAVKQVTASDVVFFALDSAGHARVAASTLP
                     RDAALTAFIQGYGSRASDSTLARAGTAEMPEANMPRAEVSLGNEAFVGQGGALLSAGG
                     TPLGGFVALRSRALELAGFRDLTIRIVLAGLVGLLLAGVFSYLVAHRISRPVLSLVAA
                     TRRAAEGDYATPIEAAGPDEIGQLAEAFGVLLAELREKQSLVNLLREREEQRGLSLAG
                     AERTVAVGGGLTDGRIAPGQVFAGRYEILAALGQGGMGVVYKARDRELDDMVAVKLVR
                     TDLLAIDPMAIERFKDEVRLARRISHRNVARTHDFGEAEGLYYVTMEFVAGTPLRDLI
                     QARHRLPVSATVSVGKQLCRALEAAHEEGIIHRDIKPQNIMVAADGLVKVMDFGIARP
                     VERQKAMTQTGLVIGTPDYMAPEQLLGEPVDPRVDLYAAAVVLYECLTGRRPHEADSA
                     LALVGVKLAQDPLPPHEVSEDVPMELSRVIVRALSRNRDDRPATAARLYDELVHAVEE
                     PSSISSS"
     gene            complement(3246..4244)
                     /locus_tag="VFB89_00065"
     CDS             complement(3246..4244)
                     /locus_tag="VFB89_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25737.1"
                     /translation="MRTSWLALLCLLAACGNPAGPVNSDPGTGTPSPSTPTPNTSVVT
                     VGGCQVFPGDNAWNRDVSADPVDPASSALLSQMSPDSALHLDLGTTEEYYGIPFVVVP
                     ASQPLVPISFGTDGADYSDESDPGPMPIPLDVPIEGGSTADPNPSSGDRHVLVVQQGD
                     CILYELYNTVRTGSGFMVSSSARWDLKINAIRPAGWTSADAAGLPILPGLLKYDEVAA
                     NHLTHALRFTVPRARRAYMAPAGHCGQYTDPTLPAYGLRVRLRADFDLSPYTRDARAI
                     LGALKKYGLIFADQGSAWYVTGTSDPRWADALDQLRQYKVHGRDFEVVQMGFITSC"
     gene            4432..4902
                     /locus_tag="VFB89_00070"
     CDS             4432..4902
                     /locus_tag="VFB89_00070"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HZM25738.1"
                     /translation="MVTMAMPVVTEAHRRLDRLVGSWKGEERLYPSPFDTQGGNAIAR
                     VHNQRALDGFAVVQDYEQERDGKVRFRGHGIFRWDGEDGAYTLHWFDSLGLPPNEFRG
                     QFEGDVLTLVNAGPQGMTRAVFEFGARSYSYRMQVSPDGINWFPFMEGAYTRED"
     gene            4908..5270
                     /locus_tag="VFB89_00075"
     CDS             4908..5270
                     /locus_tag="VFB89_00075"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZM25739.1"
                     /translation="MDKVNLAQKLSQIREHWRPKIIGELNGQEVKLVKCRGEFVWHHH
                     EIEDELFLVVRGRLRIDFVDRSVELGPGELLIVPHGVEHRTVADEEVEVLLFEPAGVR
                     NTGNVDDPTLTAPQGVRL"
     gene            5280..5384
                     /locus_tag="VFB89_00080"
     CDS             5280..5384
                     /locus_tag="VFB89_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008632889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HZM25740.1"
                     /translation="MGKGDRKSRRGKVYRGTYGKRRPRKKKAAKSAPK"
     gene            5381..5956
                     /locus_tag="VFB89_00085"
     CDS             5381..5956
                     /locus_tag="VFB89_00085"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HZM25741.1"
                     /translation="MTTPVRCAWARTELGIAYHDAEWGVPVHDDQLLFEFLILEGAQA
                     GLSWETILRKRTRYRQVFEGFDPARVSRFTAHRVARLLEDPGIVRNRLKVESAVTNAR
                     SFLEVADEFGTFDGYVWRFVGGRPRLNRWRRMTQVPARTAESDALSKDLSSRGFRFVG
                     STICYAFMQAVGMVNDHTVDCFRFRALSPRR"
     gene            6015..7061
                     /locus_tag="VFB89_00090"
     CDS             6015..7061
                     /locus_tag="VFB89_00090"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZM25742.1"
                     /translation="MRLLPLLASIALAGAPGAPDALAQAPCNPAFQPVADTVRKMVDD
                     LKLDGAALIIHLKGKTVCEAYVGRFGPETMMPIVSAAKWISAAAILALVDEGRVKLDD
                     PVSHYLPYFKGDKKAITVRQLLSHRSGLPNYLPCMFQAQLHLDDCVHQIAEKSTLSAP
                     PGKEFAYGGASYSVAGRVAEVAAGATWAQVFATRLAQPLGLEHTGYGNTANPMLSEGQ
                     VFSSAADYVTFLQMVLDNGMHGNKRVLSEAMIEEMTRDQTVGVTARISPHGDQTYGLG
                     CWRDVVDSTSGRALLLTSPGAGGFLPWVNRTRGMVGVLAVFDRIDRIGPFFPSVLLAA
                     RNGAIALDSTTSAQ"
     gene            7058..7633
                     /locus_tag="VFB89_00095"
     CDS             7058..7633
                     /locus_tag="VFB89_00095"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZM25743.1"
                     /translation="MRAAVRQSVQRSQWLTELAQLGQRIGELASPLDRRQLGWSPPEG
                     GWGAGQLLEHLCLANDSYVDRIRTAMYHAHARLPTDDPDWRPSLLGGWLARSITPGTR
                     KLPAPRMYRPAPVARPGVLEAFLHGQRALVDLVDTASRFDLTRSRTSSPVSRLIRLNL
                     GDCFAIPIVHEQRHLQQLEQICRHPAFPTWE"
     gene            complement(7652..8659)
                     /locus_tag="VFB89_00100"
     CDS             complement(7652..8659)
                     /locus_tag="VFB89_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25744.1"
                     /translation="MNKYPPQRTSDSGRIPWLRPAQLGALRLLALLGAVALAGAALLF
                     SVVWAKEGFGREDLPRCAIGLLILLGAQLSWWLLCRRLAPPASAPVLGAGIGVLLVPW
                     LIYAAFGVRTVVNTFRGSWLSRRASVRSYREAAIVWRDFDGPVGLALELDLDVPARLH
                     GSLLPPRLAYADSVGFSADDYFSSPFHQLEGRQLTQPVFQAIDPGVERSRLASGKPVH
                     LSYRLYPGYVRRLDPGLRVCIDTAAVERSSRDRGLEATRSLAASWLFAGPGGLTVDLS
                     QTLTKELRTQSRWATTRAGWDSLLQRLTPPFLTAAGYVACKGQAEAWEGEQCWCAPPQ
                     P"
     gene            8687..9757
                     /locus_tag="VFB89_00105"
     CDS             8687..9757
                     /locus_tag="VFB89_00105"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF009897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HZM25745.1"
                     /translation="MTLDIAIRKAATLKPKPAEDSLGFGRYFTDHVFVMDFAPDHGWH
                     DARIVPNEPLGLEPAAAVLHYAQAIFDGLKAFRGADGRVRTFRLDDHCARLNRSAKRL
                     CMPPIDPGWLHESIVELIRVEQDWVPRGSGTALYIRPTMIASEAFLGVRPADRYTYFV
                     LLSPVGGYYSSKKDPVKIWVEMRHVRAAPGGLGGVKAASNYAASLPAAEEAKQRGFDQ
                     VLWLDALEHRWIEEVGTMNLFVRIGDEVLTPPLDDTFLAGITRDSVLRLLRDWGTKVT
                     ERRVSIDELRHAHAHGDLAEVFGTGTAAVISPVGLLGFPDGALTIGDGKAGPVAQRLS
                     DALTGIQYARAEDRHGWMMPIT"
     gene            complement(9750..9920)
                     /locus_tag="VFB89_00110"
     CDS             complement(9750..9920)
                     /locus_tag="VFB89_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25746.1"
                     /translation="MRPQPERAIHPAEAVIALALPSVVLSDRSGRAEMITLSPATAAE
                     LSAMVAGYLGLK"
     gene            complement(10006..10398)
                     /locus_tag="VFB89_00115"
     CDS             complement(10006..10398)
                     /locus_tag="VFB89_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25747.1"
                     /translation="MPESYPYWHARLEHATGDEIIALVSSVDLAAEPTPADAGDRARE
                     LAFLSHALSEVQRRGDTGLAIQLEARMKALELHPDGTPAVRRPSIATRVETFLRQESE
                     QRGTSWVVLVTLLVLFFAALVIYGPEFK"
BASE COUNT         1898 a         3432 c         3558 g         1814 t
ORIGIN      
        1 cattgaagga tcgctcgggc cgtgcgaagg cggaccacgg gaagagttcg cgacggattc
       61 gattgcgtcg ccccgaacag gtcgagtgcg aggtcaccgg tgaatcgccc gcccagcacg
      121 ctcgaaatgt gaatcgcgcc gcctaccagc gactgccgca ccgcagcgct gaagctgccg
      181 tcccgagtcg gggcacccgc gtccgtcagg acgaattggg ggacgtccac tacgttaccc
      241 cggccgctgt tgtagaacgt gttgaccaac acgcggccga agaactcgaa ttggacgcgg
      301 ctcttggtgc gcacctccga tgcggcctgc gtctccagct ggcgccgcag cagctcgatc
      361 gcctcctcgg cgcggcggag tcgctcctcg agtgaatcgc gagatgttgt agcggaatcg
      421 gtttgctgcg cggagagagt cagggtgccg ctcagcaggg tagctaacgc gaggacagca
      481 atcctgcttg acgcacccaa gcggggtagt acgttgcggg gcatgcggca ggccagatcg
      541 aaaattgcgg ccgtcgtcct gtgggcgatg ctggccccgg gcgcgctcac agcccaggga
      601 tcggtcagcg gacgggtgac aattcaggag cgcgagggtg aagcaacaaa agacctcgcg
      661 accgctgtta tatacctcga gacgtccggc agcgccatcg gcccggctcg cgacgccgag
      721 gccgaaatcg ccatgcatga caaaacattc ataccgcacg tgcaagtggt tcccgcgggc
      781 gcgtcggttg ccttcccgaa tcaggaccct ttcaggcaca acgtcttctc caactcaagc
      841 gccggaccct tcgatctcgg cctcacgcca agaggccagt cgtcgaaaaa tcagtttcgc
      901 aagtcggggg tgtacgcgat ctactgcaac attcactcgc gcatgagtgc cttcgtggtg
      961 gcggtggcga gcccgtacta cacgcagccg ggcgcggacg gttcgttcac catttccgcg
     1021 gttcccgcgg ggcgttacgt gctgcacgcc tggcacgagc gcgccgggga gaccacgcag
     1081 acgctggagg tgccggcagc cgggttgcag agtttggcag cggttctcga cgcccgtggc
     1141 taccggcccg ctcagcacaa gaacaagttc ggccaagact acgcggcgag tgacgacgac
     1201 gagcggtact agcgggctga cctggcggat ctttttcggg gccgctggcg tcgttgccat
     1261 agtgctgctc ggaacgctgt tgctcgcctg ggcgtcagtc cgacgcactg ccgggaccga
     1321 gctcgaccga agccttgccc ggacccgcga actggtcgtc acgtttcttc agggagaccg
     1381 ccggagcctg tcccagagtg ctgcggtctt tgtccagaat cccaactttc gcgcgctgct
     1441 cgaaaatccc gccccgggcg acatgctgga ccagtcgctc gaggccgcgc atttgctggg
     1501 cgccaccttc gtccagataa cagacgcgtc cggtgtacgg ctcgcccgaa gtgacgagcc
     1561 gcaggccgct ctggtgcagc tggctcgttc gccgctgatc gcgggggcac tggagggcga
     1621 tatgcgcgcc ggcttcggtg tgtccggcga ttcgatcctg tttcaggccg ttgctgtacc
     1681 gattcccggt gccgggcccc aggtcgtccg cggctcactc atggcagcga aggcgatcga
     1741 cagcaccctg gcgagcgcgg tgaagcaggt gaccgccagc gacgtggtgt tctttgcgct
     1801 cgattcggcc ggtcatgcac gcgttgcggc ctccacgctt ccgcgtgacg cggccctcac
     1861 cgctttcatc cagggctacg ggtcgcgggc gagcgacagt accctggctc gagcgggcac
     1921 agccgagatg cccgaagcaa acatgcctcg cgcagaagtg tcactgggca atgaggcctt
     1981 cgtcggccag ggaggcgcgc tgctatcggc tggaggcacg ccgctgggcg gattcgtcgc
     2041 gcttcggagc cgggcgctcg agttggccgg cttccgcgac cttaccatcc gcatcgtcct
     2101 cgccggcctg gtcggcctgc tgctggccgg cgtgttctcg tatctcgtcg cccatcggat
     2161 cagccgtccc gtactgagcc tggtcgcagc gacccggcgg gccgccgaag gcgactatgc
     2221 gacgccgatc gaagccgcag gtccggacga gatcgggcag ctggccgaag cgttcggggt
     2281 gctcctcgcc gagctgcgcg agaagcagtc gctggtgaat ctgctgcgcg agcgcgaaga
     2341 acagcgggga ctctcactcg ccggggcgga gcgcactgtc gcggtgggcg gcggcttgac
     2401 cgatgggcga attgctccgg gacaggtctt cgcgggacgc tacgagatcc tcgccgcgct
     2461 gggacagggg ggcatgggag tggtatacaa ggcgcgcgac cgtgagctgg acgacatggt
     2521 ggccgtgaag ctggttcgca ccgatctgct ggccatcgac cctatggcga tcgagcgctt
     2581 caaggatgaa gtccggttag cccgccggat ctcgcatcgc aacgttgccc gcacccacga
     2641 tttcggcgag gccgagggac tgtactacgt cacgatggag ttcgttgcgg ggacgccgct
     2701 cagggatctg attcaggcgc ggcaccggct tccagtcagc gcgacggtgt cagtcggcaa
     2761 gcaactatgc cgagcgctgg aagcggcaca cgaggaagga atcattcacc gcgatatcaa
     2821 gccgcagaac atcatggtcg cggccgacgg cctggtaaag gtgatggact tcggtatcgc
     2881 acgcccggtc gagcgccaga aagcaatgac gcagacgggc ctcgtgatcg ggacaccgga
     2941 ctacatggcg ccggagcagc tgctgggcga gccggtggat ccacgggtgg atctgtatgc
     3001 cgcagccgtg gtgctctacg agtgtctcac cggccggcgg ccccacgagg cggactcggc
     3061 gcttgctttg gtgggtgtaa agctggcgca ggatccgcta ccgcctcacg aggtgtctga
     3121 agacgttccg atggagctat ccagggtgat cgttcgtgcc ctatcgagga atcgggacga
     3181 tcgccctgcg accgcagcac ggctctacga tgagctggta cacgcggtgg aggagccgtc
     3241 gtcgatcagc agctcgtgat gaatcccatt tgcacaacct cgaaatcgcg gccgtgcacc
     3301 ttatactgac gcagctggtc cagagcatcc gcccagcggg ggtctgacgt tcccgtcaca
     3361 taccaggcgc ttccttgatc ggcgaagatg aggccgtact tcttgagcgc cccgaggatc
     3421 gcccgagcat cgcgggtgta gggcgatagg tcgaagtcgg cgcgcagccg gactcgcagg
     3481 ccgtaggccg gaagggtggg atccgtgtac tggccgcagt ggccggccgg cgccatgtat
     3541 gcccgccggg ctcgcggcac ggtaaatcgc agggcgtgcg tgagatggtt cgcggcgact
     3601 tcgtcgtact tgaggagacc gggtagaatg ggaagcccgg cggcgtcggc cgaggtccag
     3661 ccggcgggac gaatcgcgtt gatcttcaag tcccatcgag ccgacgagct caccatgaag
     3721 ccgctgccgg tacggacggt gttgtacagc tcgtagagaa tgcagtctcc ctgctgcacc
     3781 accagcacgt gccgatctcc cgacgacggg ttcgggtcgg ccgtgcttcc tccctcgatg
     3841 ggcacatcga gcgggatcgg catcgggccg ggatcgctct cgtcggagta gtcggcgcca
     3901 tcggtcccga agctgatggg aaccagcggc tgagatgccg gcacgacgac gaacgggatt
     3961 ccgtagtact cctccgtagt cccgaggtcg aggtgcaaag cgctatcggg cgacatctgc
     4021 gacagcagcg ccgacgatgc ggggtcaacc ggatcggcgc tcacgtcacg gttccaggcg
     4081 ttgtcgcccg gaaaaacctg gcagccgcct accgtgacca ccgacgtgtt cggagtgggc
     4141 gtggagggcg aaggcgtgcc tgttccagga tcggagttga ctgggccggc gggattgccg
     4201 caagctgcca gcaggcatag cagagccaac cagctcgtgc gcatgtaggc tccggtccaa
     4261 agactcggca agagacggac ttggcgcgtg gagtcgcgag caccacacaa tgggcggggc
     4321 aataagtatg ctcgggtgac ctgctccgtt tttacattcg ccccttccgg gttctgtaga
     4381 accgactcag attggcgagc ggcttcgacc gcccaaccag gagggatcac gatggtgacg
     4441 atggcaatgc cggtggtgac ggaagcgcat cgccggttgg atcgactggt gggttcgtgg
     4501 aaaggtgagg agcgactcta tccgtcgccg ttcgacactc agggcggaaa cgccatcgcg
     4561 cgggtgcaca accagcgcgc gctcgacggg ttcgccgtgg tccaggacta cgagcaggaa
     4621 cgggacggca aggtcaggtt tcgcggacac ggaatcttcc ggtgggacgg tgaggacggc
     4681 gcctacacgc tgcactggtt cgactcactc ggtcttccgc ccaacgaatt ccggggtcag
     4741 ttcgagggag atgtactcac cttggtcaac gccggccccc aagggatgac tcgagcggtg
     4801 ttcgagttcg gcgcccgatc ctacagttac cgcatgcagg tgtctcccga cgggatcaac
     4861 tggttcccct tcatggaagg cgcctacacc cgggaggatt gacgcgcgtg gacaaagtca
     4921 atctcgcgca gaagctctcc cagattcgcg agcactggcg gcccaagatc atcggcgagc
     4981 tcaacggcca ggaagtgaag ttggtaaaat gccggggcga gttcgtctgg catcatcatg
     5041 aaatcgagga tgagctgttt cttgtggtac gcgggcggct ccggatcgac ttcgtcgaca
     5101 gatcggtcga gcttgggccg ggtgagctac tcatcgtacc tcacggcgta gagcatcgga
     5161 ccgtagccga tgaggaagta gaggttctgc tgttcgagcc agccggcgtc cgcaacacag
     5221 gtaacgtcga cgaccccact ctcaccgcgc cgcaaggagt gcggctgtaa ggagctgtca
     5281 tgggcaaggg cgatcgcaag tcacggcggg gcaaagtcta tcgcggcacc tacgggaagc
     5341 ggcggccgcg aaagaagaag gcagccaaga gcgctccgaa atgaccactc cggtgcgctg
     5401 cgcctgggcg cgcaccgagc tgggtatcgc ctatcacgat gcagagtggg gagtaccggt
     5461 ccacgacgac cagctgctgt tcgagttcct gatcctcgag ggagcccagg ccgggctgag
     5521 ctgggaaaca attcttcgga agcggacccg gtatcggcag gtgttcgagg gcttcgatcc
     5581 agctcgggtc agccgcttca ccgcccacag ggttgcgcgc ctgctcgagg atccgggcat
     5641 cgttcgcaat cggctcaagg tcgaaagcgc ggtcaccaac gcccgttcgt tcctcgaggt
     5701 ggcggacgag ttcgggacgt tcgatggcta tgtgtggcgg ttcgtgggag gacgccccag
     5761 gctgaaccgc tggcgccgaa tgacccaggt gccggcccgc accgctgagt cggacgcctt
     5821 gagcaaggat ctctcgagcc gcggctttcg cttcgtcggg tcaaccatct gctacgcctt
     5881 catgcaagcc gtcgggatgg tcaacgatca cactgtagac tgcttccggt ttcgtgccct
     5941 gagtccccgg cgctgatcac ctgccgcccg ccgccatctc gcaacccgag tggtggcggc
     6001 tccgtatggc ttgcatgcgc ctccttcctc tgttggcctc gatagcgctg gctggtgccc
     6061 ctggcgcccc cgacgccctt gcccaggctc cttgcaatcc cgcattccag cccgtcgcgg
     6121 acacggtgcg gaagatggtc gacgacctca agctcgacgg cgccgcactc atcatccacc
     6181 tcaaaggcaa gacggtttgc gaggcgtacg tgggacggtt cggtcccgag acgatgatgc
     6241 cgatcgtgtc ggccgcgaaa tggatcagtg ccgctgcgat tctcgcattg gtagacgagg
     6301 gcagggtcaa gctcgacgac cccgtgtcac actatctccc gtacttcaag ggagacaaga
     6361 aagccatcac cgttcgccag ctcctgtcgc acaggtcggg actgcccaac tacctgccct
     6421 gcatgttcca ggcgcagctt cacctcgacg actgcgtcca tcagatcgcc gaaaagagca
     6481 ccctgtcagc gccccccggg aaagagttcg cctacggcgg cgcctcctac agcgtggcgg
     6541 gtcgcgtggc agaagtcgcg gcgggcgcca cctgggccca ggtctttgcc actcgtctgg
     6601 cgcagccgct cgggctcgag cacacggggt acggcaacac cgcgaacccg atgctctcgg
     6661 aagggcaggt tttctcgtcc gcggcggatt acgtgacctt tcttcagatg gtgctcgaca
     6721 acggcatgca cggaaacaag cgggtgcttt ccgaggcaat gatcgaagaa atgacccgag
     6781 accaaacggt gggggtgacc gcacgtatct cgccgcacgg tgatcagacc tatgggctcg
     6841 ggtgttggcg ggatgtggtg gactcgacct cagggcgggc cctgctgctc accagcccgg
     6901 gcgccggcgg gtttcttccc tgggtcaacc gcacccgggg aatggtcggc gtgctggccg
     6961 tgttcgatcg gatcgaccgg atcgggcctt tcttccctag cgtgctactg gcagccagga
     7021 acggcgcgat cgcgctcgac agcacgacat cggcgcaatg agagccgcgg ttcgccaatc
     7081 ggtacagcgg tcgcagtggc tcaccgaact cgcccagctt gggcagcgga tcggtgagct
     7141 ggccagcccg ctcgatcgcc gtcagctcgg ctggtcgccg cccgagggtg gctggggcgc
     7201 gggccagctg ctcgagcatc tctgcctcgc aaacgacagc tacgtcgacc gcatcagaac
     7261 tgccatgtac catgcccacg cacgccttcc cacagacgac ccggactggc ggccgagcct
     7321 gctgggtggc tggcttgcgc gcagcatcac acccggcacg cggaaactgc cggcacctcg
     7381 gatgtatcgg cctgctccgg tggcccggcc cggagtgctc gaggctttct tgcacggtca
     7441 acgcgcactg gtggatctgg tggacaccgc gagccgattc gacctcacgc ggagccggac
     7501 cagctcaccc gtgagccggc tcattcgtct caatctgggc gactgcttcg ccatccccat
     7561 cgtgcacgag cagcgacacc tgcagcagct cgaacagatt tgccggcatc ccgcgtttcc
     7621 cacctgggag tgactgcttc gctcgcggcc tttatggctg cggcggcgca caccagcact
     7681 gctcgccttc ccaagcctct gcctggccct tgcaagcgac gtaacccgcg gcagtcagga
     7741 atggcggcgt cagccgctgg agcagggaat cccatccggc acgcgtggtc gcccagcgac
     7801 tttgggtccg caactccttg gtgagcgttt gactcaggtc cactgtgaga ccgccggggc
     7861 cggcgaacag ccagctcgcg gcgagggatc gagtcgcttc caacccgcga tcgcggctcg
     7921 agcgctctac ggcggccgtg tcgatgcaga cccggaggcc aggatcgagt cgtcgcacat
     7981 agccgggata gaggcgataa ctgagatgca cgggcttccc gctcgcgagg cgagaccgct
     8041 cgacgccagg gtcgatcgcc tgaaacacgg gctgggtcaa ttgccgtccc tccaactggt
     8101 ggaagggcga cgagaaatag tcgtccgcgc tgaagccaac gctatcggcg tatgcaagcc
     8161 ttggcggcag caggctgcca tgaagccgag ccgggacgtc gaggtccagc tcgagcgcga
     8221 gcccgaccgg gccgtcaaaa tcgcgccaga caatggcagc ctcgcgatac gatcgcaccg
     8281 acgcgcgacg ggacagccaa ctgcctcgaa atgtgtttac gacggtccgg accccgaatg
     8341 ccgcatagat gagccacggc accagtagca cgccgatgcc ggctccgagc acaggcgccg
     8401 atgcgggggg agcgagcctg cgacacagca gccaccagga gagttgagcg cccagcagga
     8461 tgagcaaccc aatcgcgcag cgaggaaggt cctcccgccc gaatccttcc ttggcccaga
     8521 caacgctgaa cagcagggcc gcccctgcca gggcgacggc gccgagcaac gcaagcagcc
     8581 gcagggcgcc caactgtgcc ggtcgtagcc aggggattct gccgctatcg ctggtgcgtt
     8641 gggggggata tttgttcatg ctggcacctc aggggggaag ggcaacatga cgctcgacat
     8701 cgcgatccga aaggcggcca cgctcaagcc caagccggcg gaagactcgc tgggcttcgg
     8761 ccggtacttc acggatcacg tcttcgtgat ggacttcgcg cccgaccacg ggtggcacga
     8821 tgcccggatc gttcccaatg agccgctcgg gctcgaaccg gctgccgccg tgctgcacta
     8881 cgcgcaggcc atctttgacg gcctcaaagc gttccgtggg gccgacggcc gcgtccgcac
     8941 gttccgcctg gacgatcatt gcgcccggct caatcggagt gccaagcgac tgtgcatgcc
     9001 gccgatcgac ccaggctggc tgcacgaatc gatcgtcgag ctgatccggg tcgagcagga
     9061 ctgggtgccc cgcggctcag ggaccgcgct ctacatccgg ccgacgatga tcgcgagcga
     9121 ggcgtttctc ggcgttcgcc cggccgaccg ttacacctac ttcgtactgc tgtcacctgt
     9181 aggcgggtat tacagctcga agaaggatcc ggtcaagatc tgggtggaga tgcggcacgt
     9241 gcgcgctgcg ccgggcggac tcgggggcgt gaaggccgcc tccaactacg ccgccagtct
     9301 tcccgctgcg gaggaagcca agcagcgggg cttcgaccag gtactgtggc tcgacgcgct
     9361 ggagcatcgc tggatcgaag aggtcggcac catgaatctg ttcgtcagga tcggcgacga
     9421 agtgctcacg ccgccgctgg acgatacctt cctggccggc atcacccggg acagcgtgct
     9481 tcggctgctg cgcgattggg gcaccaaggt gacggagcgc agggtatcga tcgacgagct
     9541 gcgacacgca cacgcgcatg gcgatctggc ggaggtgttc gggaccggaa ccgccgcagt
     9601 catctcaccg gtcggactgc tcggcttccc cgacggggcg ctcacgatcg gtgacggcaa
     9661 agccggtccc gtcgcgcagc gacttagcga cgccctgacc ggcattcagt acgccagagc
     9721 tgaggatcgg cacggctgga tgatgccgat tacttgagac ccaggtaacc tgccaccatc
     9781 gcgctgagct cggccgcggt tgccgggctc agcgtgatca tttccgcgcg cccgctccgg
     9841 tcgctcagca cgacgctcgg gagcgcgagg gcaataaccg cttccgcggg gtgaattgcc
     9901 cgctcaggct gagggcgcat gagctcggac cgacctgcgg ccagaagctg cttgagccgc
     9961 gccagcgtcc aacgccgcag cctgccgctc cgagtggagc ccttctcact taaactccgg
    10021 gccgtagata accagggcag cgaagaaaag caccagcaga gttacaagca ccacccagct
    10081 agtgccgcgc tgctcactct cctgtctcag aaacgtctca acgcgagtcg cgattgatgg
    10141 tcgccgtact gccggcgttc cgtcgggatg aagctcgaga gctttcatcc gagcctcgag
    10201 ctggatggcc agcccggtgt cgccacggcg ctgtacttcg ctgagcgcgt gcgacagaaa
    10261 ggctaactct cgcgcgcgat cgcccgcatc ggcgggggtc ggctcggcgg ccaagtctac
    10321 gctgctgacc aaggcgatga tctcatcgcc ggtcgcgtgt tccagcctag cgtgccaata
    10381 cggatagctt tctggcatgg ccccaaaaaa ctacgacagg ggccaccgac cgccagcccc
    10441 cgctgggatc cacgctcctc tctctgtgtc agacgcgaat ttgcgaaacg attcgccggc
    10501 gccctgctca acgtcgacgc cgttctcaag ggccccacca gttcatttgg cggacgatcg
    10561 aagcccgccg gtctttcgag gagaacccga gcagtggcga tctgggtagc cgccgccgca
    10621 accgtgctgg cgctgccgcc cgcgccggcc tttccgcagc agcgcgcggt cgacaccggg
    10681 atcgccgtgt ggtacttcaa gc
//
LOCUS       DASRJQ010000004         2321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2804181, whole genome shotgun sequence.
ACCESSION   DASRJQ010000004 DASRJQ010000000
VERSION     DASRJQ010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2804181"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..535
                     /locus_tag="VFB89_00120"
     CDS             224..535
                     /locus_tag="VFB89_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25748.1"
                     /translation="MIVARHLYHPLPGKSAEVLKQRQLACDVRERIGLPRGRILTRVR
                     GADGLAEVIWEWDYPDAAAYDREMEAQNASDEFMAVRRHMRMLIERMDPVLLQVAESP
                     A"
     gene            634..1566
                     /locus_tag="VFB89_00125"
     CDS             634..1566
                     /locus_tag="VFB89_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZM25749.1"
                     /translation="MRDGVQGGSVANQRLSFGLLTFLRGSWDDLVALWQELDALGFDS
                     VWLPDHVGAGMPSYEAWTALAGLACKTSQIRFGTLVTSTAFRNPVLFAKQVVTLDHLS
                     HGRLELGLGAGYDPQDTDHAMTGLSIWEPRERVQRFQESLEIIDRLLHGETLTYLGRY
                     HQVTGATLGARCLQVPRPRLTIAGYGPTMLRLVARYADTFNAGDRDSRMLVEPRAVLA
                     ATRERYDRLQEFCLQLGRDPRTLRRSIYRYCAPPTEDPWASVDAFTDFVGRYREIGVS
                     EFIFAYAPGQRERPKTFERIAMEVMPSLRDSEGR"
     gene            complement(1624..1893)
                     /locus_tag="VFB89_00130"
     CDS             complement(1624..1893)
                     /locus_tag="VFB89_00130"
                     /inference="COORDINATES: protein motif:HMM:NF021255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin Xre/MbcA/ParS toxin-binding
                     domain-containing protein"
                     /protein_id="HZM25750.1"
                     /translation="MALGVDKRTLYRWISGDNYPQREARKRLQQLVEVHHALLLMWKN
                     MDAARIWLHRPSPYLGHTEPIELVRAGRYDRVEAALEAINSGFAS"
     gene            complement(1960..2256)
                     /locus_tag="VFB89_00135"
     CDS             complement(1960..2256)
                     /locus_tag="VFB89_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25751.1"
                     /translation="MKERGGSYRQVQAAARELKETILDKYPTATFRLSRAADGPRSWN
                     VWTMVDIEDLDDVSDLVTERALDMRVEHGIPIHVVPIRGSAEPSELPAAIRRTG"
BASE COUNT          393 a          758 c          744 g          426 t
ORIGIN      
        1 cgtcaaggat gtgatcgcgg ggtacgccga catcggcatg gacgagctga tcctcaagcc
       61 ctgcctcgcc gatctggacc aggtcgaccg actggccgat tgcctgccgt agcgccgctc
      121 gccgcgactt gaggatgcac cggaaacgtc acggcgagag gtagtcagcg cgataccgcg
      181 ggccatggtg cagcacagtg gtccgctgga tgggagtgaa gccatgatcg tcgcccgtca
      241 cctgtatcat cctctcccgg gaaagtcggc cgaggttctg aagcagcgcc agctggcctg
      301 cgatgtacgc gagcggattg gcctgccgcg ggggcgcatc ctgacgaggg tacgcggtgc
      361 ggatgggcta gccgaggtca tctgggagtg ggattaccca gacgcagccg cgtatgaccg
      421 cgagatggag gcccagaacg ccagcgatga gttcatggcg gtacgccggc atatgcgcat
      481 gttgatcgag cggatggatc ccgtgttgct tcaggtcgct gagtcacccg cgtgacgatc
      541 cctctcacgt cgccccggcg ctacactgcg aggtctcctg agacaggttg taaggtgcag
      601 gtcgtccgcc gggccgaatc tggcaacgct tgaatgcgcg atggcgttca agggggcagc
      661 gtggcaaatc agcggctttc cttcggattg ctcaccttct tgcgcggcag ttgggacgac
      721 cttgtcgcct tgtggcagga gctcgacgcg ctcggctttg acagtgtctg gttgcctgac
      781 catgttggcg ccgggatgcc gtcgtatgag gcgtggacag cgctggccgg cctggcctgt
      841 aagacgagcc agatacgctt cgggaccctc gtgacgtcca cggcgtttcg caatccagtg
      901 ctgttcgcca agcaggtcgt caccctcgat cacctctcac acggccggct cgagctcggg
      961 ctgggggccg ggtacgaccc ccaggacacc gaccacgcca tgaccggctt gtcgatctgg
     1021 gagccgcgcg agcgcgtgca gcgcttccag gaatcgctcg agatcatcga ccgactgttg
     1081 cacggcgaga cgttgaccta tctgggtcgc taccaccagg tcaccggggc cacactcggt
     1141 gctcgctgcc tgcaggtgcc acgcccacga ctcacgatcg ccgggtacgg gccgaccatg
     1201 ttgcgtctcg tcgcccggta cgccgacacg ttcaacgcgg gcgaccgcga cagcaggatg
     1261 ctcgtcgagc ccagggcggt ccttgccgcg acccgcgagc gatacgaccg actgcaggag
     1321 ttctgtctgc agctcgggcg cgacccacgg acgctgcggc gctcgatcta tcgctactgc
     1381 gcgccgccga cggaagaccc gtgggcgtcc gtcgatgcct tcacggactt tgtcggccgt
     1441 tatcgcgaga tcggggtcag cgagttcatc ttcgcctacg caccggggca acgcgagcga
     1501 ccgaagacgt tcgagcgtat cgcgatggag gtcatgccgt cgctccgtga ttcggaggga
     1561 cgatgaccgg cttggttggc ggctaccata tcgacccttc aaatcgcgga caatgatgac
     1621 cgatcagctc gcgaagcccg agttgatcgc ctccagcgcc gcctcgacgc ggtcgtagcg
     1681 gccggcccgg accagttcaa tcggctcagt gtggcccagg tacgggctcg ggcggtggag
     1741 ccagatccgc gcggcgtcca tgttcttcca catcagtagg agcgcatggt gcacctcgac
     1801 gagctgctgg agccgcttgc gcgcttcgcg ctgagggtag ttgtcgccgc tgatccagcg
     1861 gtacagcgtc cgcttgtcga cgccgagcgc catcgccagc ccgcgctcgt ctaacccaag
     1921 gtcagtctga agctgcccga tttgttcgtt cataagacat catccagtcc gccggatcgc
     1981 ggccggaagc tcactgggct ccgcactacc gcgaatcgga acgacgtgaa tcgggatgcc
     2041 atgctctacc cgcatgtcca gcgcccgctc agtcaccaga tcggaaacgt cgtcgaggtc
     2101 ttcgatgtcg accatcgtcc agacattcca ggagcgcggc ccatcagccg ccctcgaaag
     2161 tctgaatgtc gccgtcggat acttgtcgag gatagtctcc ttcagctccc tggccgctgc
     2221 ttgcacctgc cggtacgatc cgcctcgctc cttcatcgga gagcctcctg cctaaccact
     2281 agccaggtga acctgaaagc ccccacccgc acgtcgcgtg c
//
LOCUS       DASRJQ010000005         5732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3659402, whole genome shotgun sequence.
ACCESSION   DASRJQ010000005 DASRJQ010000000
VERSION     DASRJQ010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3659402"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VFB89_00140"
     CDS             <1..518
                     /locus_tag="VFB89_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator SirA"
                     /protein_id="HZM25752.1"
                     /translation="RGTTRRLETPLGTLYVTVTEDEKGQPFEVFMSLGKAGGALMADV
                     EALGRLISLALRSGIPMKEIYRQLRGISSDRTIGLGANKVLSVPDAVGIAIERWMQDK
                     QGVQQELIPGLESSTAEAPVVQQVTRGGEQMTLGGMQETLSGACPDCGSQLEFAEGCM
                     KCHVCGFSECG"
     gene            complement(589..1065)
                     /locus_tag="VFB89_00145"
     CDS             complement(589..1065)
                     /locus_tag="VFB89_00145"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 family protein"
                     /protein_id="HZM25753.1"
                     /translation="MSPLAVLAFAFGIGIVAGLRSMTAPAVVSWAAHLGWLDVSHTWL
                     AFLGYRWTPYILSVLAAGELIADKLPTTPSRKQAGPFGARIVSGALSGAAVVVGGGQA
                     WALGAIAGAVGGIVGALGGYAARTGLVRALKVPDFVIALLEDLVAVGGGLFLVSRF"
     gene            1180..2832
                     /locus_tag="VFB89_00150"
     CDS             1180..2832
                     /locus_tag="VFB89_00150"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZM25754.1"
                     /translation="MASDLDVAFPVLGKKDLQALEARGRPRPVKAGEVLFAEGDRNFC
                     FFVVCEGSIEIIDNSRDTPRVIAVHHPGQFTGDVDMLTGRAALVTGRVAQDGKVLALS
                     AAELRRAVDELPEAGETIVKAFLMRRTLLLDQGFEGVKIIGSRFSPDAHRLRDFAARN
                     GIPFKWIDLDSDEQAEIMLRQLGVPTSATPIVLGRQGRWHRNPSLADFARCAGLTPTL
                     EEDHVYDLIVVGAGPAGLAASVYAASEGLDVLAADSLAAGGQAGTSSRIENYLGFPAG
                     ISGKDLTQNALLQAQRFGARITVPCEVRSLGIDGGERIVTLADRTRLRARCVLVATGV
                     EYRRLDVPRFTDFEGAGIYYAATEMEAKLCRGEEVVVVGAGNSAGQAIVYLARYAQRV
                     HVVVRGPDLGARMSRYLVDRIEGLDNVTVYRNAAVSSLEGNGHLSAVNVKIASNPPTR
                     FDTKALFLFIGAGANTAWLRGCVELDGKGFVLTGTALPPGVAEADRWRAAGRAPFLLE
                     TSVPGVFAAGDVRSGSVKRVASAVGEGAMACTLVHAHIARPA"
     gene            2890..3174
                     /locus_tag="VFB89_00155"
     CDS             2890..3174
                     /locus_tag="VFB89_00155"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HZM25755.1"
                     /translation="MAAQCRHLVEVRDVAPLTPQGCEECLRTGGRWLHLRLCQECGHV
                     GCCDNSPGKHATKHFHSSGHPIMRSFEPGEDWSWCYVDELFIEPAVKPAR"
     gene            complement(3184..4233)
                     /locus_tag="VFB89_00160"
     CDS             complement(3184..4233)
                     /locus_tag="VFB89_00160"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HZM25756.1"
                     /translation="MIRKAAVAAALMGAWLWSPSPARSQHAPANLKIERLDPRFDALI
                     PPDAAVERVANGIVWSEGPLWDPVDQSLLFSDVPRNGVYRWKEGVGVTQVIAGSGYTG
                     RGLFTGREPGSNGLVFDRQGRLVRCQHGNRRIVRREVDGSETVLADRYQGRRLNSPND
                     LVYAPNGDLYFTDPPFGLAGTFNDPAKELPFQGVYRLSRDGVLSAVITDLKAPNGLGF
                     SPDGKLLYVSSSDRERPTWFVYPVRDDGTVGPGRVFAEATAWVGPGEGVADGLKLDEQ
                     GNVFAAGPGGIHVFAPDGTRLGRIETAAPTGNLNWGEDGSVLFIAANHSILRLRTTTR
                     GFIAPGRDESAARRR"
     gene            complement(4230..4670)
                     /locus_tag="VFB89_00165"
     CDS             complement(4230..4670)
                     /locus_tag="VFB89_00165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HZM25757.1"
                     /translation="MLTPLPASTRSAIMAAGNSTTRRSFLRTAGVALSATTLVGWRDE
                     LPAMLDDGSITAFTLIHGIPGKEDELKQHLLSLSAPTRAEPGCIKYDLYQSPKQRHEF
                     LRFEVWASMAALEAHKQTPHLKASFEKRQREGWTTEIVLWERVS"
     gene            complement(4642..5067)
                     /locus_tag="VFB89_00170"
     CDS             complement(4642..5067)
                     /locus_tag="VFB89_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25758.1"
                     /translation="MRNSVMIGTIVLIGLLLLPRIGRAQGNAAPTTVPDPLALYPQNY
                     TVLFENDRVRVLDFRLAKGASEKTHRHPANVAVFLKDVKIRFTLPDGQTKIREARAGE
                     VSYSEATAHASENIGDADAHGILVELKQSNHADATPGVH"
     gene            5253..>5732
                     /locus_tag="VFB89_00175"
     CDS             5253..>5732
                     /locus_tag="VFB89_00175"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HZM25759.1"
                     /translation="MAEPKPKTPRRKKRAAEPASRGVAAREVSSGTPPAEIGRLQERI
                     AADGGTVLTAYRDPLGGNWQVFAALPIESVEPTPYQRDLSQPHVARLTDAIDRLGRFL
                     DPIIVVPAAEGRYWTPNGNHRLAALRGMGARSVVALVVPEPEVAHRILVLNTEKAHNL
                     "
BASE COUNT          992 a         1813 c         1908 g         1019 t
ORIGIN      
        1 ttcgcggcac cacgcgccga ctcgaaaccc ccctcgggac gctctacgtg accgtcactg
       61 aggacgagaa ggggcagccc ttcgaggtct tcatgagcct gggcaaggca ggcggcgcct
      121 tgatggcgga cgtggaggcg cttggcaggc tcatcagcct ggcactccgc tccggcattc
      181 cgatgaagga gatctatcgc cagctgcgcg ggatcagctc ggatcgcacg atagggctcg
      241 gggccaacaa ggtgctctcg gtgccggatg cggtgggcat cgcgatcgag cggtggatgc
      301 aggacaagca aggcgtgcag caggagctga ttccggggct cgagtcgtca accgcagagg
      361 cgcccgtggt gcagcaggtc acccgcgggg gcgagcagat gacgctcggc ggaatgcagg
      421 agaccttatc gggtgcctgt ccggactgcg gctctcagct cgagttcgcc gagggatgta
      481 tgaagtgcca cgtgtgcggc ttcagcgagt gcgggtgatc gtggtcatcc gcagatagcc
      541 gggtcgccga cagcgcccgc tcgccactgg tgagcgggcg ttgtggtctt agaagcggga
      601 gaccaggaac agcccgcccc ccaccgctac cagatcctcc agcagggcga tcacgaagtc
      661 cgggaccttc agcgcccgaa ccagcccggt ccgcgcggcg tatccaccca acgctccgac
      721 gatcccgccc accgcgccgg caatcgcgcc aagagcccac gcttggccgc ctccgacgac
      781 caccgcggca ccggagagtg cgcctgagac aatgcgggcg ccgaaaggcc ccgcctgctt
      841 gcggctgggt gtagtcggca gtttgtcggc gatcaactcg ccggccgcca ggacgctcag
      901 gatgtacggc gtccaccggt atcccaggaa tgccagccag gtatggctga cgtcgagcca
      961 gcccaggtgc gcagcccagc tgaccacggc cggcgccgtc atcgaccgca acccggcgac
     1021 gattccaatc ccgaatgcaa acgcaagaac ggctaggggg ctcatataac gccctcaagc
     1081 tgtgcatggc cgatcgggac ggcaagggga cagccctgcc gtcgctacat ttcaccgagg
     1141 aactcggtcg tgcatgggta ataagagtgg agattggcga tggcgagcga cctggacgtt
     1201 gcattccccg ttctcggtaa gaaagacctc caggccctgg aggcgcgcgg tcggccgagg
     1261 ccggtcaagg ccggcgaggt tctcttcgcc gaaggcgatc gaaacttctg cttctttgtc
     1321 gtctgcgagg gctcgatcga gataatagac aattcgcgcg acacgcctcg cgtcatcgcg
     1381 gtccaccatc ccggtcaatt cactggtgac gtggacatgc tgaccggacg cgcggcactg
     1441 gtaaccggcc gggtggccca ggacggcaag gtgctggcac tgagtgccgc ggagctgcgt
     1501 cgagcagttg acgagctgcc cgaagcgggc gagacgatcg tgaaggcgtt cctcatgcgg
     1561 cgaacgctgc tgctcgatca ggggttcgag ggcgtgaaga ttatcggctc gcgcttctcg
     1621 cccgacgcgc atcgcttgcg cgacttcgca gcccgaaacg gcattccctt caagtggatc
     1681 gatctcgatt ccgacgagca ggccgagatc atgcttcggc agctgggcgt ccccacgtca
     1741 gccacgccga tcgtgcttgg ccgtcaggga cgctggcacc ggaatccatc gctggcggac
     1801 tttgcccgct gcgcagggct taccccgact cttgaggaag atcacgtcta cgatctgatc
     1861 gtggtcgggg cggggccggc tggtctggcg gcttccgtgt acgctgcatc ggaaggactg
     1921 gacgtgctgg ctgctgattc tctcgcggcg ggcggtcagg caggaaccag ctcccgaatt
     1981 gagaactatc tcgggtttcc cgccgggatc tccggcaaag atctcaccca aaatgcattg
     2041 ctgcaggccc aacgctttgg cgcccgcatc accgttccct gcgaggtccg ctcgcttggc
     2101 atcgacggtg gcgagcgcat cgtgaccctg gcggacagga ctcgactccg ggcccgttgt
     2161 gtcctggtgg ccacgggcgt cgagtatcgc cggctcgacg ttccgcgctt caccgatttc
     2221 gaaggggcgg ggatctatta cgccgccact gagatggaag ccaagctttg ccgcggcgaa
     2281 gaggtggtgg ttgtgggggc ggggaactcg gcagggcagg ccatcgtgta cctcgcgcgt
     2341 tatgcgcagc gggtgcatgt ggtggtccgc ggtccggacc tcggcgctcg catgtctcgg
     2401 tatctggtcg atcgaatcga ggggctcgac aacgtgacgg tgtaccgcaa cgccgcggta
     2461 agcagcctgg aaggaaacgg ccatctatct gccgtcaacg taaagatcgc ttcgaacccg
     2521 cccacgcgct tcgacaccaa ggccctgttc ctcttcatcg gggccggtgc gaacactgct
     2581 tggctccggg gatgcgtcga gctggatgga aagggattcg ttctgactgg gactgccttg
     2641 ccgcctggcg tcgcagaggc ggatcgctgg cgcgctgcgg gccgggctcc tttcctgctc
     2701 gagacgagcg tgcccggcgt gttcgcggcc ggtgacgtgc ggagcgggtc ggtaaaacgg
     2761 gtggcgtccg cagtaggcga aggagcgatg gcatgcacgc tggtccacgc gcacattgcg
     2821 cggccggcct aaaagaggac atcgccggct cggacagaac cggccggcct cgatctagga
     2881 gattgaccga tggcagcgca atgcaggcat ctggtggaag tgcgagacgt ggccccgctt
     2941 acgccccagg gttgcgagga gtgccttcgc acgggcggac ggtggcttca ccttcgcctt
     3001 tgccaggagt gtggccacgt gggttgttgc gacaattcgc ccggcaagca cgcgaccaag
     3061 cacttccact cgtccggaca tccgatcatg cggagcttcg agccgggtga ggactggagc
     3121 tggtgctacg tggacgagtt gttcatcgag ccggcggtta aaccagctcg atagcctcga
     3181 gtctcagcgt cggcgtgccg ccgattcgtc gcgacccggg gcgatgaatc cgcgagtagt
     3241 ggtgcgcagc cgaaggatcg agtgattcgc ggctatgaag agcacgctac cgtcctcgcc
     3301 ccagttcaaa ttgccggtgg gggctgcggt ctcgatccgg cccagccggg tgccatcggg
     3361 tgcgaacacg tggataccgc cggggcccgc ggcgaagacg tttccctgct catccagctt
     3421 gaggccatca gccacgcctt ccccggggcc aacccatgct gtcgcctcgg cgaacacgcg
     3481 ccccggtccc accgtcccat cgtcgcggac cggatatacg aaccatgttg gacgctcccg
     3541 gtccgagctg ctgacgtaga gcaacttgcc gtcgggcgag aagccaagac cgttgggcgc
     3601 cttgaggtcc gtgatcacgg cgctcaggac gccgtcgcgg ctgagtcgat agacgccctg
     3661 gaacggcagt tccttggccg gatcgttgaa cgtacccgca agcccaaaag gagggtcggt
     3721 gaagtaaaga tcaccgttgg gcgcgtagac caggtcgttg ggactgttga gccgtcttcc
     3781 ttggtaccga tcggccagga cggtctcact gccgtcgact tcgcgcctta cgatgcgccg
     3841 gttgccgtgc tgacagcgga ctaggcggcc ttgtcgatcg aatacgagcc cgttcgaacc
     3901 gggttcccgg ccggtgaaca ggccacgacc ggtatagccg ctgcccgcga taacctgcgt
     3961 gactccgact ccctccttcc agcggtacac cccgtttcgc ggaacatccg aaaacaggag
     4021 cgactggtcc accggatccc agagtggccc ctcgctccac acgattccgt tggcgacccg
     4081 ctcgaccgcc gcgtccggag gaatgagcgc atcgaagcgg ggatcgaggc gctcgatctt
     4141 gagattggcc ggcgcatgct gagagcgcgc aggggagggg gaccacagcc aggctcccat
     4201 caaagcggcg gcaaccgcgg ctttacggat cacgacacgc gctcccagag aacgatttct
     4261 gtggtccaac cctctcgctg tcgcttttcg aacgaggcct tgaggtgcgg tgtctgtttg
     4321 tgagcctcga gagccgccat gctggcccac acctcgaagc ggagaaactc atgtctctgt
     4381 ttgggagact gatacaggtc atacttgata cagcctggct cggctcgggt cggcgctgaa
     4441 agcgagagca gatgctgctt gagctcgtcc tccttgccgg gtatcccgtg aatcagtgta
     4501 aacgcggtga tcgagccatc gtcgagcatc gccggaagct catcgcgcca gcccacgagg
     4561 gtagttgcgc tcagtgcgac ccccgccgtg cgcaggaagc tccgtcgggt ggttgagttc
     4621 cctgctgcca tgatcgcgct cctagtggac gccgggagtg gcgtcagcat ggttcgactg
     4681 cttgagctcg accagaatcc catgagcatc agcgtccccg atgttctccg atgcgtgcgc
     4741 agtcgcctcg ctgtaagaga cctcgccggc gcgggcctcg cgaatcttcg tctgtccatc
     4801 gggcaaggtg aaccggatct tcacgtcctt gaggaacacc gcgacgttgg ccggatgacg
     4861 gtgggttttt tccgaagcgc ctttggcgag gcggaagtcc aggactctga cccggtcgtt
     4921 ctcgaagagg accgtgtagt tctgcggata cagggcgagg ggatctggga ccgtggtagg
     4981 cgcagcgttc ccttgtgctc ggccgattct cgggagcagc agaaggccga tgagcactat
     5041 cgttccgatc attacgctgt tgcgcatcag gccatctcct gtccaattgg tcgccgacat
     5101 ggctttcgca tgcatgcgat aatggcgacc gcggcctccg agttcccgcg ggccaaccgc
     5161 cgcggcgggg caccgctccc cccaaggaga gcagtcggcg ccacgcggat ccggccgatg
     5221 gcggaatcgc ctgcctcgtg cttctttccc tcatggccga gcccaagcct aaaacgcccc
     5281 gacggaagaa gcgtgcggcg gagccggctt cccgcggagt ggctgcgcgg gaagtttcgt
     5341 ccggaacccc tccggcggag attggccgcc tgcaggagcg gatcgccgcc gatgggggca
     5401 ccgtcctcac tgcataccgc gaccccctcg gcggcaactg gcaggtgttt gccgcacttc
     5461 cgatcgaatc ggtcgagccc accccatatc agcgcgatct ctcccagcct cacgttgccc
     5521 ggttgaccga cgcgatcgat cggctgggcc gctttctgga cccgatcatt gtggtacccg
     5581 cggcggaagg ccgttactgg acgcccaacg gaaatcatcg gctggcggca cttcgcggga
     5641 tgggcgcccg ttcggtggtg gccctggttg ttccggagcc cgaagtggcg catcggattc
     5701 tggtgctcaa caccgaaaag gcgcacaatc tc
//
LOCUS       DASRJQ010000006         8928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1664679, whole genome shotgun sequence.
ACCESSION   DASRJQ010000006 DASRJQ010000000
VERSION     DASRJQ010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 8928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8928
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1664679"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VFB89_00180"
     CDS             <1..307
                     /locus_tag="VFB89_00180"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HZM25760.1"
                     /translation="ALFSTVHGAGRVMSRTAAAGKRHRKTGRILTPGQITPAMMQSWV
                     REKGVILRGGGLDEAPQVYRRLPEVLAAQGGTVEVLHTLRPLIVVMAGAGEVDPYKD"
     gene            334..915
                     /locus_tag="VFB89_00185"
     CDS             334..915
                     /locus_tag="VFB89_00185"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HZM25761.1"
                     /translation="MNGGVAAVNFAVAFLWLGWILYWWASSRNVKPTQWREPLRSQLA
                     HRAPLLLGALLFALPRLLPRVLRARFVQADSFIALLGAAVLVAAGLGFSVWARHHLGS
                     NWSAHVVVKEGHALIRTGPYRRLRHPIYTGILLAFLGTAVTIGEWRALVAFFLMFLSF
                     GIKSRAEENRMLATFPDYAQYRRESSAIIPFIW"
     gene            complement(994..1947)
                     /locus_tag="VFB89_00190"
     CDS             complement(994..1947)
                     /locus_tag="VFB89_00190"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HZM25762.1"
                     /translation="MSLACGGSDLTLPSEGRPANVAVVGGNGQTANGGQPLPDPIVVR
                     VTDGASRPVAQIRVAFLVTSGGGSTNPDTATTDSDGRASARWTLGAATGSQQVEARVV
                     GSDAVKATFTGTASPINSGPKRTTTTINSANPSPSFPTQPVVVAFSVSSSDGTPTGSV
                     TVTDGSDSCTASAPGGQCSLAPATAGSKTLTASYSGNSAFSPSSGTTDHQVVRAETSA
                     SLTSSENPSHRDEEVTFKASVTSSFKTPQGSVRFVEGSCGDPTRSWATETLDGNGGAS
                     FSTRSLSPGTHSMVACYEGSDIFASSESNVVPQKVSKRDHD"
     gene            complement(2107..3495)
                     /gene="sthA"
                     /locus_tag="VFB89_00195"
     CDS             complement(2107..3495)
                     /gene="sthA"
                     /locus_tag="VFB89_00195"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF003585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HZM25763.1"
                     /translation="MDYQFDLVSVGSGPAGQRAAVQAAKLGKRAALIERRRLLGGVCV
                     DTGTIPSKTFREAILSITCGSLDGAPGRRPETRPTADMLLQRVNSVVAREVRVIEDQL
                     RRNDVEVISGEASFKDPHTLLIRGENGWREITAASVVIGVGTQPGPVPGVKPDGEVII
                     SSDDIMNLKRIPHSMVVVGAGVIGIEYASMFGALGVQVSLMERRDRPLEFLDHEIVDE
                     LVHEMRRRNVILRPGEAVARIDVIEGPPRRVLVELESGKKITSDLALFAIGRIGATDM
                     LNLPAAGLTADERGRIKVDEHFRTAVPHIFAAGDVVGYPSLAATSSEQGRLAACHALG
                     VPAKAMAENFPVGIYAIPEISMTGPPEHELTKQRVPYEVGLARYREIARGQILGDDTG
                     LLKLIFHRDDRRLLGVHIIGTSATELLHIGQAVLGLGGGLDYFLDTVFNYPTLAECYK
                     VAALNAANKLAN"
     gene            complement(3527..5251)
                     /locus_tag="VFB89_00200"
     CDS             complement(3527..5251)
                     /locus_tag="VFB89_00200"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZM25764.1"
                     /translation="MITRDDLLPMPLLAELPVEQVDAIAARAADLTLEKDEWLLQEGE
                     QAAFFFLLSGHISVYKEMGGERRTINHFKPPEHFGEVPLMLNSPALASLCADEPSRVA
                     RLEADDFQELILTNRHLSDQLFRTMVERLTKARDVVAEVPHRQITLVGQRWDPACHEL
                     RNFLARNRVTVTWIDLADEGAAQQVPGGFTEGTSCPLLVLEDGTRLTTPSLRQAADHI
                     PTIRTRPSVRADEGCYDVVIVGGGPAGLAAGVYGASEGLKTVLIERFAPGGQAGSSTR
                     IENYLGFPVGLTGEELSLRALRQARRFEAEIVSARDAVSLTPPGADGDDSTHMIGLDG
                     DDCVRARAVVLATGASWRRLAAEGVDRLIGRGVYYGAARTEAQRTRGKHIHLLGGGNS
                     AGQAAMWFSNYAERVSMLVRGPSLAASMSQYLIDQLKSRRNVEIRFDTEVLAVDGHDH
                     VEQVTLRDRSRDTRQTEPTGGVFILIGADAETDWLPPAIVRDTPGYICTGRDMLQALE
                     ERILQGTDTSGLGPSWNLARDPYLLETSVPGIFAAGDVRHGSIKRCASGVGEGSMAIA
                     FVHQYLTH"
     gene            complement(5330..5770)
                     /locus_tag="VFB89_00205"
     CDS             complement(5330..5770)
                     /locus_tag="VFB89_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25765.1"
                     /translation="MQLTFLAALALAAASLDSYLPSPAPAHQGTDLAARLTGTWHGRR
                     TTPISIRADSFTMSWKRAPDGHMAGTVATAGEAKYPVNVVWSSDTAFIYESGPHMSAL
                     LHERVITRATVHFDGNQLNGTFEARPTKYEGKTTTGTFTALRSA"
     gene            complement(5832..>8928)
                     /locus_tag="VFB89_00210"
     CDS             complement(5832..>8928)
                     /locus_tag="VFB89_00210"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein ndvB"
                     /protein_id="HZM25766.1"
                     /translation="TEAAVFQEIAGYLFYANPSVRAPQAELLRNRGSQPALWAAGVSG
                     DWPILLATIDSPDGLPTLRRLLAAHRYWRRRGMMVDLVVLNTRPPSYLQDLQDKITAM
                     IFSASDPGVIDQPGGVFARRRDLLSADAVGMLRATARVHVPCDGRAVGRILESVMARG
                     AMPREAPAAIPTPARAADRTAPPALRALFRVIGDTSSRGFSDSAPRITSLQIPPVASG
                     SGVWNDGVAPFENGFGALTPAGDYEMRVGGDHVPPAPWANVIANPRGGFVVSERGAGF
                     TWCDSSYFFRLTPWHNDPVGDPINDVIYLCDPESGAVWSATPAPVRHDGQYTVRHGTG
                     VSSFKHEHDGIATLLTLGLPEQDPVKLSLLEITNLSDRPRRLTITAFVEWTVGVTREH
                     TQHQVHTSFDPELGAILARNYFDPVFSNHVAFCAISEPVIAWTADRREFLGRNGTVAA
                     PAGLSGDLAGTIGVGIDPCAALRTTVELEPNQTRELVVVLGADRGDLAAQRVIAEYRA
                     PDRARAAIHRAVAGWAERLSVISVRTPEPSFDAMVNRWSLYQALSCRMWARSALYQSG
                     GAYGFRDQLQDCMAFVYAEPGLARRHLLVAAARQFVEGDVQHWWHPQTGRGVRTRFSD
                     DLVWLPYVVDHYVRITGDASVLAEQVPFLAQRPLEPHEHEVYDRPEVSAERASVYEHC
                     VRALRRACTEGPHGLPLIGDGDWNDGMNRVGIEGRGESVWLAWFLITTLRAFAEHAEA
                     RADAGTASIFRSRADAYAGAIEDHGWDGDWYRRAYFDDGTPLGSSGSEECRIDSIAQS
                     WSVISQAAPPERQARAMQSFREHLVREDARMLLLLTPPFDRTSHDPGYIKGYVPGVRE
                     NGAQYTHAGLWAVLATAQQGDGARAFELFQMLNPLTRTRTPSDVATYKVEPYVVAADV
                     YAAPGHFGRGGWTWYTGSASWMYRVALEGILGFTRRGNTLTIRPCVPPGWPEFTIEYQ
                     YGASRYVIVVRSPGALGTAPAMVTLDGATLTDPVVRLIDDGTRHEVLVEPGTAAGVER
                     P"
BASE COUNT         1515 a         2846 c         2997 g         1570 t
ORIGIN      
        1 cgccctgttc tcgactgttc atggggccgg gcgggtgatg agtcgcaccg ccgcagcagg
       61 aaagcgtcat cggaaaaccg gtcggatcct gaccccaggt cagattacgc cggcgatgat
      121 gcaaagctgg gttcgagaaa aaggcgtcat cttgcgcgga ggtgggctgg acgaggcacc
      181 gcaggtctat cgccgccttc ccgaggtgct ggccgcgcag ggtggaaccg tcgaggtgct
      241 gcatacgctc cggccgctga tcgtggtgat ggcgggagcg ggggaggttg atccgtacaa
      301 agactgaaaa gcgtgaacgg tgaacggtaa acggtgaacg ggggcgtcgc tgcagtgaat
      361 tttgccgttg cctttctctg gctcggatgg atcctgtact ggtgggcatc ctcgcggaac
      421 gtcaagccaa ctcagtggcg cgaaccgcta aggtctcagc tggcccatcg agcgcctctc
      481 cttctaggcg ccctcctctt cgcgttgccg cgcttgcttc cgcgcgtact tcgagcgcgg
      541 tttgttcagg ctgacagctt cattgctctg ctgggggcgg cggtcctggt cgcggcagga
      601 cttggtttct cggtctgggc acgccaccac ctggggagca actggagcgc ccacgtcgtc
      661 gtcaaggagg ggcatgcctt gattcggacc gggccgtacc gacgactgcg ccatccgatc
      721 tacacgggga tattgcttgc gttcttggga accgcagtca ccatcgggga gtggcgcgcg
      781 ctggtcgcct tctttctgat gttcctatcg ttcgggatca agagccgcgc agaggagaac
      841 cgaatgctgg caacgtttcc cgactacgct caatatcggc gggaatcctc ggcaatcatt
      901 ccttttatat ggtaagagcg aggggtgacg agccggaatc gactcgttgc acctacggaa
      961 gctcaagctg gcttgagcag gtgatcgagc tgttcagtcg tggtctctct tgctcacctt
     1021 ctgcgggacc acgttgctct cactgctcgc gaagatatcg ctcccctcgt agcaggccac
     1081 catcgaatgc gtgcccggcg agaggctgcg agtagagaag gacgccccgc cgttcccatc
     1141 gagcgtttcg gttgcccatg accgggtcgg atcgccgcag cttccttcaa cgaaccggac
     1201 cgacccttga ggcgtcttga agctgctggt gacggaggct ttaaaggtga cctcctcgtc
     1261 acggtgtgat ggattttcgc tcgacgtgag agaggccgag gtttcagcgc ggaccacctg
     1321 gtggtcggtc gtgcccgagc tgggcgaaaa ggcgctgttg ccgctgtagc tcgcggtcaa
     1381 tgtcttggaa ccggcggtcg ctggcgccag gctgcattgg ccgccagggg cggaggcagt
     1441 gcagctgtcg gagccgtcgg tcaccgtcac cgagccggtc ggggttccgt cgctcgaact
     1501 cacgctgaac gccacgacga cgggctgggt ggggaacgaa ggcgacggat tcgccgagtt
     1561 gatcgtggtg gtggtgcgct tggggccgct gttgatggga gacgcggtgc cggtgaaggt
     1621 cgctttgact gcgtccgacc ccacgacgcg agcctctacc tgctgtgagc cggtggctgc
     1681 gccaagcgtc caccgggcgc tggcacggcc gtctgaatcg gtggtcgcgg tatcggggtt
     1741 ggtgctcccg ccaccggatg taaccaggaa ggcgactcga atctgggcaa cgggacggct
     1801 ggcgccgtcg gttacccgca cgacgatcgg gtcggggagg ggctgcccac cgttagcggt
     1861 ctgaccgttc cccccaacca cggcaacgtt ggccgggcgg ccttcgctgg gcagggtaag
     1921 atcgctccct ccgcaggcta aactcactac ggcgcaacat gatagagcgg tcgctaggga
     1981 ggcgagcgag acgtggcgca tacaggcttg aacggccgaa ttggtctcaa tttaacgtct
     2041 ggcgtcgccc tagtgcagtg attcaggtcg cccaagggtc aggttggggg tctgacgagg
     2101 ggtggatcag ttggccagct tattcgccgc gttcagggcc gcgaccttgt agcactcggc
     2161 cagggtcgga tagttgaaga cggtgtccag gaagtagtcg aggccgcctc cgagacccag
     2221 gaccgcttga ccaatgtgga gcagctcggt cgcgctggtc ccgatgatgt ggacgcccag
     2281 caggcgccgg tcatcgcgat ggaagatcag cttgagcagc ccggtgtcgt cgcccagaat
     2341 ctgcccacgg gcgatctcgc ggtagcgtgc caagcccacc tcgtaaggga cccgctgctt
     2401 ggtcagctcg tgctcgggcg ggccggtcat tgaaatctcc ggaatcgcgt agatacccac
     2461 ggggaagttc tccgccattg ccttggcggg aacgcctaat gcatggcagg cggcaagccg
     2521 cccttgctcg ctcgacgtgg cggccaggct cgggtaaccg acgacgtctc ccgccgcgaa
     2581 gatgtggggg acggcagtgc ggaaatgctc gtccaccttg atgcggccac gttcgtccgc
     2641 ggtaagcccg gcggccggca gattgagcat atcggtggcg ccgatccgtc cgatggcgaa
     2701 cagcgccagg tccgaagtga tctttttccc cgactcgagc tcgaccagca cccgcctggg
     2761 tggaccctcg atgacgtcga tccgagccac tgcctcaccc ggccgcagga tcacgttgcg
     2821 tcgccgcatt tcgtgcacca gctcatcgac gatttcgtga tccagaaact cgagcggacg
     2881 gtcgcgccgc tccatgagcg acacctgcac gccgagggcg ccgaacatcg aagcgtactc
     2941 gatgccgatg acaccggcgc ccaccacgac catcgaatgg gggatgcgct tgaggttcat
     3001 gatgtcatcg ctcgagatga tgacttcgcc gtcgggcttc acgcccggca ccggccctgg
     3061 ctgcgtgccc accccgatca ccacgctggc ggcggtgatc tcccgccatc cgttctcgcc
     3121 gcgaatcagg agggtgtggg ggtccttgaa cgacgcctcg ccggagatca cctcgacgtc
     3181 gttgcggcgc agctggtctt cgatgactcg gacctcccgc gccaccaccg agttgactcg
     3241 ctgcaacagc atgtccgcgg tcggtctggt ctccggccgg cgaccgggtg cgccgtcgag
     3301 cgagccgcag gtgatggaga gaattgcctc gcggaatgtc ttgctgggaa tcgttcccgt
     3361 atccacgcag acgccgccca gaagccggcg ccgctcgatc agcgccgcgc gtttgcccag
     3421 cttggcggcc tgaaccgcgg cccgctggcc tgccggcccg cttccgacac tcacgagatc
     3481 gaattggtag tccatgttgt ggagaatgaa ttgcgattgt aacggttcag tgggtcaggt
     3541 attggtgcac gaaggctatc gccatgctgc cctctccaac tcctgacgcg caccgcttga
     3601 tggagccgtg gcggacgtcc cccgccgcga agatgccggg aacgctggtt tcgagaaggt
     3661 agggatcacg ggccaggttc caactggggc cgagcccgct ggtgtctgtc ccttgcagga
     3721 tccgctcctc gagggcttgg agcatgtcgc ggccggtgca gatatagccg ggggtgtcac
     3781 gcacgatcgc aggcgggagc caatcggtct ccgcgtctgc gccgatcagg atgaagacac
     3841 cacctgtcgg ctcggtctgc cgggtgtctc tggaccgatc gcgcagcgtg acctgctcca
     3901 catggtcgtg gccgtccacg gctagaacct cggtgtcgaa cctgatctcg acattgcgcc
     3961 ggctcttgag ctgatcgatc agatattgcg acatgctcgc ggcgagcgac ggcccgcgga
     4021 ccagcatgct gactctctca gcgtagttgg aaaaccacat cgctgcctga ccggcggagt
     4081 tgcctccgcc gagcagatga atgtgcttgc cccgcgtgcg ctgcgcctcg gtacgcgccg
     4141 ccccatagta caccccgcga ccgatcagcc ggtcgactcc ctctgcggcg agtctcctcc
     4201 acgatgcccc ggtggccagg accaccgccc gagcacggac gcaatcgtcg ccatcgagtc
     4261 cgatcatgtg cgtcgaatcg tccccgtcgg cccccggggg ggtcaggctt acggcgtcgc
     4321 gagcgcttac gatttcggct tcgaaccgtc tggcctggcg caaggcgcga aggctgagct
     4381 cctcgccggt aaggccgacc ggaaagccga gatagttctc gatgcgtgtg gaactaccgg
     4441 cctgcccgcc gggagcaaac ctctcgatca acacggtctt caacccttcg gatgcgccat
     4501 agacgccagc cgccagcccg gcgggtccgc cgcctacgat cacgacgtcg tagcatcctt
     4561 cgtctgcccg cactgacggc cgggtccgga ttgtaggaat gtggtcggct gcttggcgga
     4621 ggctaggggt cgtgagccgg gtgccgtctt ccaggacgag cagcgggcac gaagttccct
     4681 cggtgaaccc acccggcacc tgttgggctg cgccttcgtc tgccagatcg atccaggtaa
     4741 cggtcactcg attgcgggcg aggaaattcc gcagctcatg gcaggcggga tcccatcgct
     4801 gcccgaccag agtgatctgc ctatgcggga cctccgccac cacgtcccgg gccttggtaa
     4861 ggcgctccac catggtcctg aacagctgat cgctcagatg ccgattggtg aggatcagct
     4921 cctggaaatc gtcggcctcc agccgagcaa cccggcttgg ctcgtcggcg caaaggctcg
     4981 cgagcgccgg gctgttcagc atgagcggaa cctcgccgaa gtgctccggg ggcttgaagt
     5041 gattgattgt tcgccgctca ccgcccatct ccttgtacac ggagatgtgt ccgctcagca
     5101 gaaagaagaa ggcggcctgc tcaccctcct gaagcagcca ttcgtctttt tcgagggtga
     5161 ggtcggccgc gcgagcggca atggcatcga cctgctcgac ggggagctcg gcgaggaggg
     5221 gcatggggag gagatcgtct ctcgtaatca ttggggaaag ctaatcccca atgtagagag
     5281 tctgctccgg ggtttttggc cgacctcctc ccttactcgg ccgcgatggt caggccgaac
     5341 gcagggctgt aaacgtgccc gtggtcgttt tgccttcgta cttggtgggg cgcgcttcga
     5401 aggttccgtt cagctggttg ccgtcgaagt gcacggtcgc acgcgtaatc acgcgctcgt
     5461 gcagcagggc gctcatgtga ggccccgact catagatgaa agccgtgtcg gagctccaga
     5521 ccacgttgac cggatacttc gcctctcccg ccgtcgcaac ggtgccggcc atgtgaccgt
     5581 ccggggccct cttccagctc atggtaaagc tgtcggccct gatggagatc ggggtcgtcc
     5641 ggcgcccgtg ccaggtcccg gtgagccgag cggccaggtc ggtgccctga tgagcaggtg
     5701 cgggagaggg gagatagctg tcgagagaag cggccgcgag agccagggcc gcgaggaagg
     5761 tgagctgcat ggggcctgcc taacctatgg gcacccatat aatgcccggg ccggagcaaa
     5821 cgtcgcccgc atcagggccg ctcgacgcct gccgcggtcc cgggctccac cagcacctcg
     5881 tggcgcgtgc cgtcatcgat caaacgcacg acgggatcgg tcagagtggc gccgtcgagc
     5941 gttaccattg ccggcgcagt gcccagcgct ccagggctgc gcaccacgat gacgtagcga
     6001 ctggccccgt actggtattc gatggtgaac tcgggccagc ccggcggtac acatggccgg
     6061 atggtcagcg tgtttccgcg gcgggtaaac ccgaggatcc cctccagcgc cacccgatac
     6121 atccagctgg ccgaaccggt ataccaggtc cagcctcccc ggccaaaatg acccggcgcg
     6181 gcgtacacgt cggcggccac gacgtaaggc tcgaccttgt aggtggcaac gtccgacggt
     6241 gtgcgggtgc gtgtgagcgg attgagcatc tgaaacagct cgaatgcgcg tgcaccgtct
     6301 ccctgttgag cagtcgcgag cactgcccag agcccggcat gcgtgtactg cgccccgttc
     6361 tcgcgcacgc caggaacgta tcccttgatg taccccgggt cgtggctggt gcggtcgaaa
     6421 ggcggggtga gcagcaggag catgcgggca tcctcgcgca ccagatgctc ccggaacgac
     6481 tgcatcgccc gagcttgtcg ctcgggaggc gccgcttgcg agatcacgct ccagctctgc
     6541 gcgatcgaat cgatgcggca ctcctcgctc ccggaggaac caagcggcgt gccgtcgtcg
     6601 aagtaggccc ggcgatacca gtcgccgtcc cagccgtggt cctcgatcgc acccgcgtat
     6661 gcatcagcgc gggaacgaaa gatggatgcg gttccggcat cagccctggc ttcggcatgc
     6721 tcggcaaagg cgcgcaacgt cgttatcagg aaccaggcga gccacacgct ttcaccccga
     6781 ccctcgatgc ccacccggtt cataccgtcg ttccagtcgc cgtcgccgat gagcggcagg
     6841 ccatgtggcc cctcggtgca cgcccggcgc aaggcgcgta cgcaatgctc gtacactgaa
     6901 gcccgctcag ccgagacctc cggccggtcg tagacttcgt gctcgtgagg ctccagggga
     6961 cgttgcgcca ggaacgggac ctgctctgcc agcaccgatg cgtcgcccgt gatccgcacg
     7021 tagtggtcta cgacgtaggg aagccagacg agatcgtccg agaagcgggt ccggaccccg
     7081 cggcctgtct ggggatgcca ccagtgctgc acgtcgcctt cgacgaactg ccgtgccgcc
     7141 gccaccagaa ggtgccggcg cgcgagtccg ggctcggcgt acacgaacgc catgcagtcc
     7201 tgcagctgat cgcggaagcc atacgcgcct ccgctctgat agagtgccga gcgtgcccac
     7261 atgcggcagg aaagcgcctg gtagagcgac caacgattca ccatcgcgtc gaacgacggc
     7321 tccggagtgc gaaccgagat aactgagagg cgctcggccc acccggcgac tgcacggtgg
     7381 atggcggcac gggcgcgatc cggggcgcgg tattcggcga tgacgcgctg agccgccagg
     7441 tctccccggt ctgcgccgag caccacgacc agctcccgag tctggttggg ctcgagctcg
     7501 acggtggtcc gcagagcggc gcaggggtcg atccccacac cgatcgttcc tgccagatcg
     7561 ccgctgagtc cggccggcgc tgcgaccgtc ccgtttctac cgaggaactc ccggcgatcc
     7621 gcggtccagg ctatgaccgg ctcactaatc gcgcagaagg cgacgtggtt cgagaagacg
     7681 gggtcgaagt agtttcgcgc caggattgcg cccagctccg gatcgaacga ggtgtggacc
     7741 tggtgctggg tatgctcgcg cgtcacccca acggtccact cgacgaaggc ggtaatcgtg
     7801 agccggcggg gacggtcgct cagattggtg atctcgagaa gcgagagctt gacgggatcc
     7861 tgctcgggca gaccaagcgt gagaagcgtc gcgatcccgt cgtgctcgtg cttgaatgac
     7921 gaaactcccg ttccgtgccg gacggtgtac tggccgtcgt ggcgaaccgg cgccggcgtg
     7981 gcgctccaga cggcgccgct ctccgggtcg cacagataga tcacgtcgtt gatcggatcg
     8041 ccgaccgggt cgttgtgcca gggtgtaagg cggaaaaagt agctgctgtc gcaccaggtg
     8101 aacccggcgc cccgctcgga gactacgaat ccgccacgcg ggttcgcgat cacgttggcc
     8161 cagggagcgg gcgggacatg atcgccgcct acccgcatct cgtagtcgcc ggcgggtgtg
     8221 agcgcgccga acccgttctc gaagggcgcg acgccatcgt tccagacgcc ggatcccgac
     8281 gcaaccgggg ggatctgcag cgacgtaatc cggggcgccg agtcgctgaa gccccggctc
     8341 gacgtatccc cgatgacgcg aaacagcgcg cgcagggccg gaggcgccgt ccgatctgcg
     8401 gcgcgggccg gggtgggaat cgcggccggc gcctcccggg gcatcgcgcc gcgtgccatg
     8461 accgattcca gaatgcgccc gactgcgcgg ccatcgcagg gaacgtgcac gcgcgcggtg
     8521 gcgcgcagca ttccgaccgc gtcggcgctc agcaagtcgc ggcgacgggc aaagactccg
     8581 ccaggctgat cgatgacgcc gggatcggaa gcagagaaga tcatggcggt gatcttgtcc
     8641 tggaggtcct ggagatagct gggcggacgg gtgttcagta ccacgaggtc caccatcatg
     8701 ccgcgccgcc gccaatagcg atgggcggcg agcaagcggc gcaaggtggg gagtccatcg
     8761 ggtgaatcga tggtggcgag caggatcggc caatcgcccg acaccccggc tgcccacaag
     8821 gccggctggg atccgcgatt tcgcagcagc tcggcctgtg gcgcccgtac cgacggattg
     8881 gcatagaaga gatatccggc gatctcctgg aacaccgccg cctcggtc
//
LOCUS       DASRJQ010000007         8748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_840784, whole genome shotgun sequence.
ACCESSION   DASRJQ010000007 DASRJQ010000000
VERSION     DASRJQ010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 8748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8748
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840784"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="VFB89_00215"
     CDS             complement(<1..952)
                     /locus_tag="VFB89_00215"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HZM25767.1"
                     /translation="MKVLIADKFEKVGVDGLKELGCTVVSLPDLTAEALPVALREHDP
                     NVLIVRGTKVSGEALRSGTSLGLVIRAGAGIDTIDVNAASDLGVFVSNCPGKNSIAVA
                     ELVMGLVLSCDRRIPDQVADLRAGKWNKGEYSKARGLHGRTLGVVGLGQIGREIATRA
                     RAFGMHVAAWSRNLTLAEAERLGLRYCQTPLEVARVSDVVSINVAANADTRNLVSEEF
                     LSAMKPGAYLINTSRGTVIDEPALLQAIRKKGLRVGLDVFHNEPAGSTGTFDSAIARE
                     PAVYGTHHVGASTEQAQVAIGHEVIRIVQEYQHSGIVPNCV"
     gene            complement(949..2181)
                     /locus_tag="VFB89_00220"
     CDS             complement(949..2181)
                     /locus_tag="VFB89_00220"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HZM25768.1"
                     /translation="MRIRPFRAIRPRPELAAAVASVPYDVVNRAEAAALAEGNPHSFL
                     HVGRSDIDLPADTDPYDSRIYSGARAALDRLLADGTLVRDQSPTLFLYRLVMDGRAQT
                     GVVACVHVDDYEHDLIRKHEKTRKDKEDDRTRHVLTLNAHAEPVFLTYRSVPAVDRLA
                     DATMRQPSLYDFTASDGVRHTVWRIADSKSWVEAFDVIPLAYVADGHHRSASAWRAGK
                     ERQSANPRHTGNEEYNWFLAVLFPQNQLRILPYNRLVKDLNGLSPAAFRERLAALGKL
                     EDTDQPIPDRPGVFTFFFDGRWERLTIDPQRINHQDAIRSLDVSLLQDLVLTPLLGIA
                     DPRTDKRVDFVGGIRGTAELERRVRSGEMALGVSLYPTTIDQLLAVADAGAIMPPKST
                     WFEPKLRSGLFVHELDGS"
     gene            complement(2269..3234)
                     /locus_tag="VFB89_00225"
     CDS             complement(2269..3234)
                     /locus_tag="VFB89_00225"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HZM25769.1"
                     /translation="MKAIRVHRLGGPEVLQYDDVPVPTPAAGQALIRMEAAGVNFIDI
                     YRRTGLYKIELPHTLGQEGAGTVEALGPGVSGVAVGDRVAFTDVPGAYAELAVAPVER
                     VVPVPSKVSSHQAAAVILQGITAHYLATATYPLQQGNVCLVHAAAGGVGLLLCQIAKR
                     RGARVIATVSTEEKAELARGAGADDVILYTRQDFEVEVKRMVGGVQVVYDSVGKTTFD
                     KGLDCLRPRGMMVLFGQSSGPVPPFDPQILNRKGSLFLTRPTLFNYIPTRDELLSRAG
                     EVLGWVADGSLSVRIGREYPLAEAASAQRALEGRETTGKVLLIPA"
     gene            complement(3263..3991)
                     /locus_tag="VFB89_00230"
     CDS             complement(3263..3991)
                     /locus_tag="VFB89_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25770.1"
                     /translation="MSGRELPPLASRVLASYGGAERWRSARSVRATLSIGGLALVAKG
                     RLPLRHVPVGAELDFPRMRIDRLAGSSSVAWLEGGSTRLVDSDGQTRTVRGDARAGFG
                     SLRRWLWWDRLDATYFLGYLLWNELTFPRLFLRSDVEWTQLDELSLRATFPSGFPTHC
                     TEQIFRFDRETGLLRRHDYTVDVFGPWARAVTIVIKHREWDGIMYACRRVATLGSARG
                     KRFNWPVLWSVEVHAWQLSKAALG"
     gene            complement(3988..4680)
                     /locus_tag="VFB89_00235"
     CDS             complement(3988..4680)
                     /locus_tag="VFB89_00235"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZM25771.1"
                     /translation="MGVALDLFQRNAFSVSVDRPSGHTHGGRVRYAFLVETYATERIK
                     VLSTWATFRDEDLPVRPHPSDQRGRSVLEHMVHQCVSENLWFRDMLGIDVGAPPLPQP
                     ETRLEFIRRYAADSGQRLTRLRGEAEAWWETEVRFFEVPRTRAWIVVRRIAHTAHHRG
                     QQTELLRMLNRSLYSTYGPTADTGGLMQQKAPTVYAYADESDLLVGEAAGGRKAALPG
                     TGDKPVTERPVA"
     gene            4727..6565
                     /gene="treZ"
                     /locus_tag="VFB89_00240"
     CDS             4727..6565
                     /gene="treZ"
                     /locus_tag="VFB89_00240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HZM25772.1"
                     /translation="MTSPPKVAESSGLLRRLPVGAEPQPRGGVHFRVWAPDRTAVEVL
                     LEGADPLQLEAEPGGYFAGIAARAAVGACYRYRLDRGDAFPDPASRFQPAGPHGPSEV
                     IDPGLFRWTDAAWPGLRLEGQVLYELHVGAFSAEGTWAAAAKELPRLAELGVTAIEIM
                     PVADFPGKFGWGYDGVNLFAPSRLYGRPDDFRAFVNQAHQAGLGVLLDVVYNHLGPDG
                     NYLGQFARSYVTERHHTPWGPAPNYDGPDAAPVREMILANAGYWIEEFHVDGLRLDAT
                     HTIYDDSPRHILSEIGRTVRERGAGRGTLVFAEDDLRRVALALPFERGGLGLDGIWND
                     DLHHAAIVAAGGKREGYYRAFEGSATELVAVTRGTLRDPGAIDPPHLVTFLQNHDQIA
                     HSARGLRLHALTSPGRWRALTAYLLLTPGTPLLFMGQEFAASSPFLFFADHEPKLAAL
                     VRRGRAEYLAQFESIADPAMQALLDDPGSPDTFARSRLDPSEVAQHPEAVALHRDLLM
                     LRRLDAAFSRQKTPRGRAFSQSALALRFAADDDLDRLLLLNLGHDLPIAEDADPLLAA
                     PPDAAWTLIWSSEDPRYGGGGTSAAARQRRLIPAESAIVLAPERRP"
     gene            complement(6516..8102)
                     /gene="cimA"
                     /locus_tag="VFB89_00245"
     CDS             complement(6516..8102)
                     /gene="cimA"
                     /locus_tag="VFB89_00245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HZM25773.1"
                     /translation="MRITIYDTTLRDGTQREGLSLSVEDKLKIARALDSLGVHYIEGG
                     WPGSNPKDAEFFIRVGSETLDQARIAAFGSTRRPGVRCDSDPNIRSLVTAGTPVVTLV
                     GKSSALHVERVLETSRDENLRMIAESVGYFRGLDREVIFDAEHFFDGWALDRDYALAT
                     LRAAADAGAHWIVLCDTNGGTLPDTISDVVRCVRERVGTRLGIHPHDDAGVAVANALA
                     AVQAGCMQVQGTINGYGERCGNVDLIPVVANLSLKLGHAVLPLEQLRRLTQVSHYVAA
                     VANQNPEAHAPYVGRSAFAHKGGIHVAAIAKLPQSYQHIDPAQVGNEPRVVVSEVAGR
                     RNVRLRAEALGLDPTRNEAEVLQWIKELEHRGFQFEAADGSFEMLLRRAAPDYRAPFE
                     LLDFTVISEKRGGQKVAAQATVKLRVCGEVVHTAAEGDGPVNALDQALRKALVPRYPA
                     LYDVRLVDYKVRIVDEHLGTAARPRVILESARGTERWSTVGCSENIIEASWLALWDSL
                     ELPLLRDAARAPVLSPTLPG"
     gene            complement(8483..>8748)
                     /locus_tag="VFB89_00250"
     CDS             complement(8483..>8748)
                     /locus_tag="VFB89_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25774.1"
                     /translation="VMERWAAEHDDFQLIASPGRRSWAVAALIPPPDRPAPLLLRAMR
                     AKGYTLAGGLGELADRVLRIGHMGDLSPAHLEVMLAQLAVCAV"
BASE COUNT         1581 a         2933 c         2714 g         1520 t
ORIGIN      
        1 tgacgcagtt ggggacgatc ccggaatgct ggtactcctg aacgatgcga atcacctcgt
       61 gcccgatcgc cacctgagct tgctcggtcg acgccccgac gtgatgcgtt ccatacaccg
      121 ccggctccct ggcaatggcg ctgtcgaacg taccggtcga gccggccggc tcgttgtgga
      181 atacatcgag tcccacccgg aggccctttt tgcgaatcgc ctggagcagc gccggctcgt
      241 cgatcacggt tccccgcgag gtattgatca gatacgcgcc cggcttcatg gccgagagaa
      301 actcttcact caccaggttc cgggtgtcag cgttggcagc gacattgatc gacaccacat
      361 cgctcacccg cgccacttcg agcggagtct ggcagtaccg caggccgaga cgctccgcct
      421 ctgccagtgt gagattacgc gaccaggccg cgacgtgcat gccgaatgca cgggcccgcg
      481 tcgcgatctc gcgcccaatc tgacccagcc cgacgacgcc gagggttcgg ccgtggagtc
      541 ctcgagcctt ggaatactcc cctttgttcc acttgccggc ccgcagatcg gcaacctgat
      601 cgggaattcg tcgatcgcac gagagcacca gacccatcac caattcggcg accgcgatcg
      661 agttcttccc cggacagttg gagacgaaga cgccgagatc ggatgcggcg ttgacatcga
      721 tggtgtcgat ccccgccccc gccctgatca cgagacccag actggtgccg ctccgcaaag
      781 cctccccgct caccttggtg ccccgcacga tgaggacgtt gggatcgtgc tccctcaacg
      841 cgaccggcag cgcttcggcc gtgaggtcgg gaagcgatac gacggtgcac cccagctcct
      901 tgagcccgtc gacaccaact ttctcgaact tgtccgcaat gaggactttc atgagccatc
      961 gagctcgtgc acgaagagtc cgcttctcag cttgggctcg aaccaggtgc tcttgggcgg
     1021 catgatggct cccgcatcgg cgaccgcgag cagctgatcg atcgtggtgg gatacagcga
     1081 taccccgagc gccatctccc ccgatcggac ccgtcgctcg agttcggccg tgccgcggat
     1141 gccgcccacg aaatcaacgc gcttgtcggt tcgcggatcg gcaataccga gcaacggcgt
     1201 cagcacgaga tcctgaagca ggctcacgtc gagcgaccga atcgcatcct gatggttgat
     1261 gcgctgagga tcgatcgtca agcgttccca ccgcccatcg aaaaagaagg taaatacgcc
     1321 cgggcggtcc ggaatcggct ggtcggtgtc ctcgagcttc ccgagtgccg caagcctttc
     1381 ccgaaacgcc gccggactga ggccgttcag atcctttacc aggcggttgt aaggcaggat
     1441 cctcagctgg ttctgcggaa atagcaccgc caggaaccag ttgtactcct cattacccgt
     1501 gtgtctcgga ttggcggatt ggcgttcctt ccccgcccgc caggcgctcg ctgaacgatg
     1561 gtggccatct gccacgtagg ccagcgggat gacgtcaaat gcctcgaccc atgacttcga
     1621 atcagcgatt cgccaaacgg tgtggcgcac gccgtccgac gcagtaaagt cgtacaggct
     1681 tggctggcgc atcgtggcgt ccgccagcct gtccaccgcc ggaaccgaac gatacgtgag
     1741 aaacaccggc tcggcgtggg cgttcaacgt gaggacgtgc cgggtccgat cgtcttcctt
     1801 gtccttccgg gtcttttcgt gctttcggat caggtcgtgc tcgtaatcgt cgacatgcac
     1861 acaggcgacc acgcccgttt gggcccgtcc gtccatcacc aggcgataga gaaacagcgt
     1921 cggcgactgg tcccgcacca gcgttccatc agcgagcagc cggtccagcg ccgctcttgc
     1981 acccgagtaa atccgcgaat cgtaggggtc ggtatccgcc ggcaggtcga tgtccgatcg
     2041 gccgacgtga aggaagctgt gcgggtttcc ctcggccagt gccgccgcct ctgcccggtt
     2101 caccacatcg tacggaacgc tcgccaccgc cgcggcaagt tcgggtcgcg ggcgaattgc
     2161 gcgaaatggc cggatgcgca tgagaagaac gtaaacggta aacagtgaac ggtgaacggg
     2221 ggctcacttc cgaaacgcag ctcccattca ccgtttaccg ttcaccgttc acgcaggtat
     2281 cagcaacact ttccccgtgg tctcccgccc ctccagcgct cgctgagcgg acgctgcctc
     2341 ggccagcgga tactcccggc ctatacgcac cgacagcgac ccgtccgcca cccaccccaa
     2401 cacttcacca gcccgtgaga gcagctcgtc gcgggtcggg atgtagttga agagggtcgg
     2461 tctcgtgagg aacagcgatc cctttcggtt caggatctga gggtcgaacg gagggaccgg
     2521 gccactggat tggccgaaaa gcaccatcat tccgcggggc cggaggcagt cgagcccctt
     2581 gtcgaacgta gtctttccga ccgagtcata aacgacctgc acgccgccaa ccattcgctt
     2641 gacctcgacc tcgaagtcct ggcgggtgta aaggatcaca tcgtcggcac ccgccccccg
     2701 cgccaactcc gccttctctt cggtggatac cgtggcgatc acgcgagcgc cacgccgctt
     2761 ggcaatctga caaagcagga ggcctacacc gcccgccgcc gcgtgcacca ggcagacatt
     2821 gccctgctgc aagggatacg tcgcggttgc gaggtagtgg gccgtgattc cctggaggat
     2881 gaccgcggcc gcctggtgcg aactcacctt tgatggaacc ggaacgacgc gctctacggg
     2941 ggcgacggca agttccgcat acgcaccggg aacgtcggtg aaggcaaccc ggtcgccgac
     3001 cgcaacacca cttacgccgg gccccagcgc ctccaccgta cccgcacctt cctggccgag
     3061 cgtgtgtggc agctcgatct tgtagaggcc ggtgcgccga taaatgtcga tgaagttgac
     3121 cccggcagcc tccattcgga tcagagcctg cccggcggct ggcgtgggca cgggcacgtc
     3181 gtcgtactgc aggacctcag gtccgccgag ccgatgtacc cgaattgcct tcatgaatct
     3241 ccccgtactg cggagcgcaa cttcagccta acgcggcctt ggacaattgc catgcgtgca
     3301 cctcaaccga ccagagtacc ggccagttga atcgcttgcc acgggcactc ccgagcgtcg
     3361 ctacgcggcg gcacgcatac ataatcccgt cccattctcg atgcttgatc acaattgtca
     3421 cggcccgggc ccatggcccg aatacatcga cggtgtaatc gtgacgccgc agcaagccgg
     3481 tctcccgatc gaagcggaag atctgttcgg tgcagtgcgt ggggaaaccg ctcgggaagg
     3541 tggcgcggag agacaactca tccagctgag tccactccac atcggatcgc aggaacagac
     3601 gggggaaagt cagctcgttc cacagcaggt agccgagaaa ataggtagcg tcgagacggt
     3661 cccaccacag ccaacggcgg aggctgccaa agccggctct cgcatctccg cggacggtcc
     3721 gcgtctggcc gtcggagtca accagacgag tagagcctcc ctcgagccag gccaccgagc
     3781 tgcttcccgc gagacggtcg atccgcattc gggggaagtc gagctcggcg ccgacgggca
     3841 cgtggcggag aggcaaacgc cccttggcca cgagcgccaa tcctccgatc gacagcgtcg
     3901 cacggaccga gcgggcagac cgccatcgct cggccccgcc gtaggacgcg agaacgcggg
     3961 acgcgagcgg aggaagctcc cgtccgctca cgcgaccggt cgctccgtca caggcttgtc
     4021 accggtgccg ggcagcgccg ctttgcgccc cccggccgcc tcgccgacga gcaggtccga
     4081 ttcatccgcg taggcataca ccgtgggagc tttttgctgc atcagcccac cggtgtcggc
     4141 ggtgggcccg taggtgctgt acaggcttcg gttgagcatc cggagcagct cggtctgctg
     4201 gccccggtga tgagccgtat gggcaattcg acgaaccacg atccaggcgc gggtcctcgg
     4261 gacctcgaag aaacgtacct ctgtttccca ccaggcctcc gcttcgccgc ggagccgggt
     4321 cagccgctgg ccggagtcgg ccgcgtagcg ccggatgaac tcgagcctgg tttccggctg
     4381 cggcagcgga ggagcgccga catcgatgcc cagcatatcc cgaaaccaga gattctcgct
     4441 cacgcactgg tggaccatgt gctccagaac gctccgacca cgctgatcac tggggtgtgg
     4501 ccggaccggg aggtcctcgt cacggaacgt ggcccaggta ctcagcacct tgatccgctc
     4561 ggtggcgtag gtttctacca gaaaggcata ccgcacgcgc cctccgtggg tgtgtcccga
     4621 aggtcggtcg accgataccg aaaaggcgtt gcgctggaac aaatcgagcg cgacgcccac
     4681 tgtcaaacac cgctgccggc acaccggcca ccacgatgtc gagccgatga cctcaccgcc
     4741 taaggttgcc gaatcgtcgg gtctcctgcg ccgccttcca gtgggcgcgg agccgcagcc
     4801 ccgaggagga gtccactttc gggtctgggc tcccgatcgc accgcggttg aggtgctgct
     4861 cgaaggtgcc gacccactcc aactcgaggc ggaacccggc ggctatttcg ccggcatcgc
     4921 tgcgcgcgcc gcagtcggcg cctgctaccg ttatcgactc gatcgaggcg acgcgtttcc
     4981 agaccctgcg tcacggtttc agcccgctgg cccgcatggt ccttcggagg tgatcgaccc
     5041 gggcttgttc cgctggacgg acgcagcctg gccagggctc cgactcgaag gtcaggtcct
     5101 ctacgagctg cacgtcggcg ccttcagcgc cgaaggaact tgggctgccg cagcgaaaga
     5161 gttgcccagg ttggcagagc ttggcgtcac cgcaatcgaa atcatgcccg tggcggactt
     5221 tcccggaaag tttggatggg gctacgacgg cgtcaatctg ttcgcgccga gccgcctcta
     5281 cggccggcct gacgactttc gagcgttcgt gaatcaggcc caccaggcgg gacttggtgt
     5341 actcctcgat gtggtgtaca accatctcgg acccgacggc aactacctgg gccagtttgc
     5401 tcgcagctac gtcacggaga gacatcacac accttggggt ccggctccca actacgatgg
     5461 cccggacgcc gctccggtcc gcgagatgat cctcgccaat gcgggctact ggatcgaaga
     5521 atttcatgtg gatggcctga ggctcgacgc cactcacacg atctacgatg actcgccccg
     5581 ccacattctc tccgaaatcg gccggacggt ccgggagcga ggcgctgggc gcggcaccct
     5641 ggtcttcgca gaagatgacc tgcggcgtgt ggcgctcgcc ctcccatttg aacggggagg
     5701 actgggactc gacggcatct ggaatgacga cctgcatcac gccgcaatcg tagcagcagg
     5761 aggaaaacgt gagggctact atcgggcctt cgagggttcg gcaaccgagc tggtggcggt
     5821 cacacgggga acgttacgag accctggcgc aatcgaccct cctcacctgg tcacctttct
     5881 tcagaaccac gatcagatcg cccactcggc tcgaggactc cggctccacg cattgacgag
     5941 ccccggacgt tggcgggcgc tgaccgccta tctcctgctc acaccaggga ccccgctcct
     6001 tttcatgggc caggagttcg cggcctcgag tccattcctt ttctttgcgg atcacgagcc
     6061 caagctcgcc gcgctggtcc gccggggccg ggcggaatac ctggctcaat tcgagagcat
     6121 cgccgacccg gcgatgcagg cgctgctgga cgacccgggc tcgccagaca cgttcgcgcg
     6181 ctcccggctc gacccgagcg aggtcgcgca gcaccccgag gccgtggcgc tccatcggga
     6241 tctgctcatg ctgcgtcgcc tcgatgccgc cttctcccgc cagaagacgc cgcgcggccg
     6301 cgcgttcagc cagtccgccc tggcgcttcg gtttgcggcc gatgacgacc tcgatcggct
     6361 gttgctcctc aaccttgggc acgacttgcc catagcggag gacgctgacc cactgctcgc
     6421 agcgccgccc gacgcggcgt ggaccctgat atggtcatcc gaggacccac gctatggcgg
     6481 cggtggtacc tctgcagccg cacgacagcg gcgtctcatc ccggcagagt cggcgatagt
     6541 actggcgccc gagcggcgtc cctgaggagg ggaagctcga ggctgtccca gagggccagc
     6601 cagctggctt cgataatgtt ctcggagcag ccgacagtgc tccaccgttc cgtcccgcgg
     6661 gccgactcga gaatgacgcg gggccgggcc gccgtgccga ggtgctcgtc tacgatgcga
     6721 accttgtagt ctaccagccg cacatcgtac aacgcgggat agcgcgggac cagcgccttc
     6781 cgcagagcct gatccagtgc attgacgggt ccgtcaccct cggcggccgt gtgcacgact
     6841 tctccgcaga cccgcagctt gactgtcgcc tgcgcagcaa ccttctgtcc accacgcttc
     6901 tcggagatga cggtgaagtc gagcagctcg aatggtgccc ggtagtcggg cgccgcgcgg
     6961 cgcagcagca tttcgaacga gccgtccgcc gcttcgaact gaaatccccg gtgctccaac
     7021 tccttgatcc actgcagcac ttccgcctcg ttcctggttg gatccagtcc gagcgcctcc
     7081 gccctgagtc gaacgttccg gcggccggcg acctcgctca ccacgactcg aggctcgttg
     7141 ccaacctggg ccggatcgat gtgctggtag ctctgtggta gcttggcaat cgcggcgaca
     7201 tggatcccgc ccttgtgggc gaaggcgctc cgccctacgt acggcgcgtg tgcctcggga
     7261 ttctggttgg caacggcggc cacatagtgg gagacctggg tcaatcggcg gagctgttca
     7321 agcggaagaa ctgcgtgccc gagcttgagc gacagattcg cgactaccgg gatgagatcc
     7381 acgttgccgc agcgttcgcc gtagccgttg atggtgcctt gcacctgcat gcaccccgcc
     7441 tgtacggccg cgagcgcgtt ggcgacggcg actccggcgt cgtcgtgagg atggatgcca
     7501 agacgtgttc cgactcgctc acggacgcac ctcacgacgt cactgatcgt atccggcagg
     7561 gtgcctccgt tggtatcaca cagcacgatc cagtgggcgc cggcatcggc tgccgcccgg
     7621 agcgtggcca gcgcgtagtc ccgatcgagg gcccagccat cgaagaagtg ttcggcatca
     7681 aagatgactt cgcggtcgag gccgcggaaa taaccgacac tttccgcgat catccgcaga
     7741 ttctcgtccc ggctggtttc cagaacccgt tcgacgtgta gcgccgagct cttgccgacc
     7801 agcgtcacga ctggtgtgcc cgcggtcacc agggagcgga tgttgggatc cgaatcgcat
     7861 cggacgccgg ggcgtcttgt gcttccgaaa gcagcgatcc tcgcctggtc cagcgtctcg
     7921 ctcccgacgc ggatgaagaa ctccgcgtcc ttgggattgg acccgggcca gccgccctcg
     7981 atgtaatgca cgccgaggga gtcgagcgcc cgcgcgatct tgagcttgtc ctcgacggag
     8041 agggaaaggc cttcccgctg ggtaccatcg cgcaaggtcg tgtcgtaaat cgtgattcgc
     8101 attgccaatc cggcgcgaag gacgaaaacg aagcggcccc ccggattcgg gaggccgcgt
     8161 ggggtcgcta ccgcaaacac tcagatcagc gcagccgccc accgcctccc gacgggagtc
     8221 gaatatgccg aatgatgctg agggggcgag gattcatgcg cacgaatgaa agtctatgca
     8281 gcctcagcgt gtcgtcaata ggcaggtaac aaaggaggga ggaacgggcg ccgaagcacc
     8341 cgtccctggc aggatattga aacgagagga tgtattgtga tgaagatggc ggcagtcttt
     8401 ttgtgaggcc cggtgccggc cgaacctcga caaccaccac tcgccaactt acctcattcc
     8461 acaagcctcg ccgtgcgcca tgtcacacgg cgcaaaccgc gagttgagcg agcattacct
     8521 cgagatgcgc gggagaaagg tcgcccatgt ggccgattcg aaggacccgg tcggcgagct
     8581 cgcccagccc tccggccagt gtatatcctt tcgcccgcat tgcccggagg agcagcggcg
     8641 ccgggcggtc cggcggagga ataagtgccg ccacagccca ggagcgccgg cctggcgagg
     8701 caatcagctg aaagtcgtcg tgctccgctg cccacctttc catgacgg
//
LOCUS       DASRJQ010000008         3266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3658571, whole genome shotgun sequence.
ACCESSION   DASRJQ010000008 DASRJQ010000000
VERSION     DASRJQ010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3658571"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /locus_tag="VFB89_00255"
     CDS             <1..1127
                     /locus_tag="VFB89_00255"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HZM25775.1"
                     /translation="IGVERGFMTRRDAIERTLTTLRFFWNSPQGEDPDATGHRGFYYH
                     FLHMDTGWRAWESELSTIDSAFLLAGMLAAGTYFDGANPEEREVRTLADQLYRRADWQ
                     WAQNAGRTVTHGWKPETGFIQYRWEGYNEALILYVLGLASPTHPLPAESYRAWTETYC
                     WKTLYGYEFLYAGPLFIHQLSHMWIDFRGIQDDFMRARGIDYFENSRRATYVQQQYAI
                     QNPLDYRGYGENCWGITASDGPGPTCMMIEGVERCFEDYTARGVPYGPDDGTIAPWAA
                     VASLPFAPEIVLPALQHFNDVYPEMTSEYGFKCSFNPTFTAEDQARRGWIAKGYYGLD
                     QGPIVMMIENYRTGFLWDLMKGCPYISEGLRRAGFTGGWL"
     gene            1680..2051
                     /locus_tag="VFB89_00260"
     CDS             1680..2051
                     /locus_tag="VFB89_00260"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HZM25776.1"
                     /translation="MRRTIAYWSTTAIVALMLLGSLSYLTGNEQVVSGFAKAGYPQHL
                     RIVLGIVKPAAAIVLLLPGLALLKEWAYAGVSFAWIMALISASSSGEPLQVVVLPPVL
                     LVLLIVSYLTRPPTRRVHSPM"
     gene            2075..2218
                     /locus_tag="VFB89_00265"
     CDS             2075..2218
                     /locus_tag="VFB89_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25777.1"
                     /translation="MQAQGQSRSVLSSGRVLSSDVTAIAFDRTGAGTLVTLSNAKSKT
                     LLR"
BASE COUNT          605 a         1012 c         1053 g          596 t
ORIGIN      
        1 tcatcggcgt cgaacgtgga ttcatgactc gccgggacgc gatagaacgg accctgacga
       61 cgctgcggtt cttttggaac agccctcagg gtgaggaccc cgacgccacc gggcaccgcg
      121 gcttctacta tcactttctc cacatggata ccgggtggcg agcctgggaa tcggagctct
      181 ccaccatcga ctcggccttc ctcctggccg ggatgctggc ggcgggcacc tacttcgacg
      241 gcgcgaaccc cgaggagcgg gaagtacgaa cgctggctga ccagctctat cgccgagccg
      301 attggcagtg ggcgcagaat gccgggcgca cagtgaccca tggatggaaa cccgaaaccg
      361 ggttcatcca ataccgctgg gagggctaca acgaagcctt gatactctac gttctgggct
      421 tggcctctcc gacacatccg ctgccggcgg agagctatcg ggcctggacc gagacgtact
      481 gctggaagac cctttacggg tacgagtttc tgtacgccgg cccgctcttc attcatcagc
      541 tctcgcacat gtggatcgac ttccgtggca ttcaggacga cttcatgcgc gcccggggaa
      601 tcgactactt cgagaacagc cggcgggcca cctacgtgca gcagcaatac gccatccaga
      661 atcccctgga ctaccgaggg tacggcgaga actgctgggg gattacggcg agcgatggtc
      721 cggggccgac ttgcatgatg atcgagggtg tcgagcgatg cttcgaggat tacaccgcgc
      781 gcggagttcc ctatggcccc gatgacggca ccatcgctcc ctgggctgcg gtggcttcgc
      841 tgccgtttgc accggaaatc gtcctgccgg ccctccagca cttcaacgat gtctatccgg
      901 agatgaccag cgagtacgga ttcaagtgca gcttcaaccc gacgttcacg gccgaagacc
      961 aggccaggag aggctggatc gccaagggat actatggtct ggaccagggt ccgatcgtca
     1021 tgatgatcga gaactaccgc accggattcc tctgggacct gatgaaaggg tgtccctata
     1081 tcagcgaggg actgcgtcgc gccggcttca cgggtggctg gctgtagccg acggctccgc
     1141 tcccgttacg gcgtacgcca ggaccgccat gattgctgcc agcgtcctcg ccccgccgta
     1201 gatcgacgcg agcgaaaggc cggcccccac gccgtggccg ctacgctcgg cggacactca
     1261 tttcgggcgc gcatggccgc ggccttcagc cgagaccgcc ccgccgctct tctggaggac
     1321 gcgatcgcca tcgggactgt gttcgctgtg gtgctggcat cgcgatgaac cgcttcgacg
     1381 ccatcgtgat cggggcgggg caagcgggcc cgtcgcggga ggcggctcac agccgcaggc
     1441 atgaccgtag ctgtgatcga acgcaagctc gtcggggaca cgtgcaccgc aacacgtgcg
     1501 acgaccgctt caatcggctg gagacatacc tgcagcagat gaaaacaaag gagaagcgtc
     1561 atggtcgtca gcagcgtaag aagtagcctc ggagatctgc ggacagaact gcgcgcctga
     1621 ttcatccggc aggcctcgcg ggctggagtc gcgaggcctg ccagacacgt ggaggcacta
     1681 tgcgtcgaac catcgcgtat tggagcacga ccgcgatagt ggcgttgatg ttgctggggt
     1741 cgctgagcta tctcaccggg aacgaacagg tcgtatccgg atttgcgaag gcgggatatc
     1801 cgcaacacct tcggatcgtc ctcggcatcg tgaagccggc cgcggcgatt gtgctcctgc
     1861 tgcccggcct cgcgctgctg aaggagtggg cgtacgcggg tgtcagcttc gcgtggatca
     1921 tggcgttgat ctcggcgtct tcctcgggcg agccactgca ggtcgtcgta ctgccgccgg
     1981 tcctgcttgt gctcctgatt gtgtcgtacc tgaccaggcc tccgacccgc agggtccaca
     2041 gcccgatgta agagagtcac aggacgaagg catcatgcaa gcccaaggtc agtcacgcag
     2101 cgtcctcagc agcggacgcg tactctccag cgatgtcacg gccatcgcat tcgaccggac
     2161 gggcgccgga acgctcgtga cgctgtcgaa cgcgaagtcg aagacactgc tgcgctagtg
     2221 gaccaggccg gcggtgcagc ctacctgtgg ggaacgtcgt ccggcgccgt gctcgccctc
     2281 gaggcggcca gtcgcctggg tggtatcaac tcggcggata cgcaacgcgc tcatcggttg
     2341 gcaagaacca cgagaaagta agggactcac cacaagcgcc tcatggagga ccaccgccgc
     2401 agacctcgta cttgcgtcgt tgtctgtcgg tcagtggaga cccagagtgc gtcattgagc
     2461 gttctcgctg cttgctgagc cattgtgcct gaaaaacatg ggctgctagt ttgcggtcgt
     2521 acccaacagg aggcactctt gccaatgagt cggcgttcgt cggttggccg ggagattcaa
     2581 gcaatcggcc gctctctagc aaacatagcc caagccctca cgcgtctcac tcggctgatc
     2641 gccgcgagca cctcacagtc tggcaatccc aggactcggg tccgtaagct gacgctctca
     2701 cgtggccggc gcgcggccct cacactgcag ggccagtaca tgggtatatg cgtagcctaa
     2761 agcccgctca aaggacacgc gtcaagggag cgaggacgac gaagggaatg cgggcggcga
     2821 tcgcgctagc gaagaagctg tcaaacggga caaagtgaac gggaggtcga gaatcggcag
     2881 gcgccttgtc gttgctggac ccttgctgtt cgccgcaagt gcgcgcgcgg agcatctcgt
     2941 cgcgtcttga atttgggtcc gcgatcgact gatcgggacg agcaccgact ggagcgcgac
     3001 cgcagcccgg atcgtgctgg gcgttgtgat gttcccccac ggcgcccaag ctactgggct
     3061 ggttcgcgat cgagtcgctc gcatccgtcg cgctcgtcac cggctttctc ggtaggctcg
     3121 cagcggcggg catcgcggcc gtcatgttgg gcgctgttac cacagtgcac gtgggcaacg
     3181 gtttcttcat gaattggggc ggcacgcagt ccggcgaggg attcgagtac cacatcctgg
     3241 ccttgggtct cgccctcgtc gtgatg
//
LOCUS       DASRJQ010000009         5234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_839511, whole genome shotgun sequence.
ACCESSION   DASRJQ010000009 DASRJQ010000000
VERSION     DASRJQ010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839511"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..3571
                     /gene="gltB"
                     /locus_tag="VFB89_00270"
                     /pseudo
     CDS             59..3571
                     /gene="gltB"
                     /locus_tag="VFB89_00270"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
     gene            3658..4284
                     /locus_tag="VFB89_00275"
     CDS             3658..4284
                     /locus_tag="VFB89_00275"
                     /inference="COORDINATES: protein motif:HMM:NF013645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25778.1"
                     /translation="MHLDLEGEANDYVGKGLSGGEITIRPYRKAAYADQTHQNLIIGN
                     TVLYGATGGKLFAAGQAGDRFAVRNSGAIAVVEGAGNHCCEYMTSGIVVVLGRAGRNL
                     GAGMSNGVAYVLDEPGTLLERVNLDMVDVVDLDAEDEDVLRRLVREHEEKTTSPRART
                     ILVEWDQYRPLFRKIAPKGAEAYMAATRAAYLQGSQPEPELALARRSA"
     gene            4304..4954
                     /locus_tag="VFB89_00280"
     CDS             4304..4954
                     /locus_tag="VFB89_00280"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HZM25779.1"
                     /translation="MLRRPSRLTILLGVLLGAAPSVACGQGTSSPDRTILGFELGFSR
                     ASFSPALGTEDREGGIIGGFIAERFGKLFALQGELLFTKKGGSVPVESQFGLVDVGVQ
                     LIYVEAPLLARVTLPVGTHLRPVVFGGGSFALSVGCEFQFEIQGTLNQARCDQPGTGL
                     ELSSTDWSGIFGGGVEYGWRRSAVRLELRRFIGLKNLVEGVEQKNRAWVVLFGINF"
     gene            complement(4944..>5234)
                     /locus_tag="VFB89_00285"
     CDS             complement(4944..>5234)
                     /locus_tag="VFB89_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25780.1"
                     /translation="YLTTLALTVTNPLTILSFAAAFAGLGLVATAGRGHLAGVTLVAA
                     VFLGSAFWWLLLSSGVGWVGRRLGVGALRAINWMSGLMLVAFGLVAVFAARS"
BASE COUNT          933 a         1666 c         1714 g          921 t
ORIGIN      
        1 cggcgtaggt gcgttctttc taccctgcga ttccgacggg ctggccgccg cgaccggtat
       61 cgtagagcag gtcctcattg gagacggcat tccctttctg ggctggcgcg atgtaccggt
      121 caacgccgcc ggcctgggtc ccaccgcgcg cgccagttgt ccgatcatcc gccaggcctt
      181 cgtggggacg ccggcgggcg cgatggacga ggacagctgg gagcgggccc tttatctcgc
      241 acgccgcgag atggagcgcc gggccggagt agcgggcctg tgcggcttct atgtggcttc
      301 gctgtcgtgc cggaccattg tgtacaaggc gctgctcaca ggcacgcatc tgcccgtgtt
      361 ctataccgat ttccgctatc ccgagtacga gacggcgatc gcaattttcc atcagcgcta
      421 ttccaccaac acacttccca gctggccgct ggcgcagccg ttccgcctgc tcgcccacaa
      481 cggcgagatc aacacgctct ggggaaaccg aaatgccatg gcgatgcggc aaccgctgct
      541 ggattccccg ctgtggggca atcaggtcga gcgcattcgg gaggcgatct ggaaagacgg
      601 cagcgactcg gcaagcctcg acaatgccat ggaactgttc gtgcgttccg gccgggatcc
      661 catccacacg ctgatgatga tgattcctca ggcatgggag aagtacccgg acctccatcc
      721 gtccatcaag gcattctacg aataccacca gtgcgtccac gagccgtggg acggacccgc
      781 ggcagtggcg ttcactgacg gaatcgcggc cggcgcgatt gtcgatcgca acgggctccg
      841 cccctgccgc tacaagatcc gccgcgatgg gttggtgatc gccggatcgg aagtgggact
      901 ggtggatctc gacccgcaag aagtcgtcga gaccggcaag gtaggcccgg gagaggttct
      961 gctggtagat actcgacggg gcgaggtgat tcgtaatctc gatgcgaagc tcgaggtcgc
     1021 tgcccgccgg ccctacgctc gttgggtggc gcgttacatg gcacgactgg aacccgaccc
     1081 ttcccgggta ccggccgggc cggatccgga aacgctgggt cggctacaac gggccttcgg
     1141 atacggattc gaagatctgc gtcacgtcct ggaacccatg ggggggacgg gagcagacgc
     1201 cgtctggagc atgggcgacg atacgcctat cccgccgctc tcgcgcatgc cccagagtgt
     1261 ctacgcgtac ttcaggcagc gtttcgcgca ggtgaccaat cctcccatcg attcgctgcg
     1321 cgaatcgatg gtgatgtcgc tccgggtcca tctcggccgc cggggctcac ctctgctcga
     1381 gcgcccgagc tacgcccgca tgttgcggat cgagcacccg atcatcctgc ccgaggagat
     1441 ggccgcgttg cacaatgtgg ccggtttttc cgcggtcacg atcgatgccg actgggatcc
     1501 gagccttggc ccggaaggac tccgtccggc gctggtgagt ctgcgccgtg ttgccgagcg
     1561 ggcggtgcgt cgaggggcca acatcctggt ggtcagcgat cgggccgtag cgccgcgccg
     1621 ggcaccgatt ccgatgctcc ttgcgatcgg tgcagtccgg cagcatctgg tggagcgggg
     1681 cctgcgcgcc agggtgggcc tggtcgcgga ggcgggcgac gcgttcgaca tccatcactt
     1741 cgccgcgatc atcggctatg gcgcggaagc cgttcaccca tggctcgccc tcgccacggt
     1801 agatgcgctg ttcgatggcc gcgagccgga acgccgccgc gatgctcacg aaacagagcg
     1861 acccagtccg gcggaggccc gcctcaagtt tcgctcggcc gccgaaaagg gattgctcaa
     1921 gatcctgtcc aaaatgggca tctccacgct ctcgtcgtac tgcggcgcac agatcttcga
     1981 ggcactcggc ctgggagccg aggtaatcga cacctgcttc tcagggaccg cttcgcccat
     2041 aggcggtatc ggattcgaag agatcgctga cgacgtgctg ggacgcttca acgcggcctt
     2101 tggcgacgac gccgagccgg ccggcgcgct tcctgattac ggccgggttc gattccggaa
     2161 ggaaggagag gatcacggct ggtcacctcc ggttgtggtc gcgctccagc aggcagtcaa
     2221 ggccggaacg tcggaggcct ataccggata tctctcgaag agtgagtctc gccgcccggc
     2281 cggaccccgc gatctgctcg cgatcagggc gggttcagcc gtgcccctcg acgacgtcga
     2341 gccggccgat gcgatccgca cccggttcat ctcgtcggcg atgtcgctcg gcgcgctgtc
     2401 tcctgaagcc catgccgcgc tctcgatcgc gatgaaccgg ataggagcaa agtcgaactc
     2461 gggcgagggg ggcgaggatc ccgcgagctt cgaatcgctc gacaatggcg ttcgcctgga
     2521 taaccgcatc aagcaggtcg cctccgcgcg ctttggggtg accgccgagt acctgatgcg
     2581 cgcagaggag ctggagatca agatcgtcca gggggccaag ccaggcgaag gaggccagct
     2641 accgggacac aaggtgaccg agttgatcgc gcggctgcgg cacgcggtac cagggatcca
     2701 gctgatctcg cctccgccgc accacgacat ctattcaatc gaggatctcg ctcagctgat
     2761 ccacgacctc aagaccatca acccactcgc acgagttgga gtcaagctgg tttccgaggc
     2821 aggcgtggga acggtcgctg ccggcgtcgc gaaggcatac gctgactacg tgctcattgc
     2881 ggggcacaac ggcggcaccg gagcgtcgcc gttgtcctcc atcaagcatg cgggggcgcc
     2941 ctgggaattg ggcttggcag aagcacaggc gaccttggtg cgaaacggac tccgccaccg
     3001 aatcgaggtg cgtaccgacg gcggcctcaa gaccgggcgc gacgtggtca tcgccgcgct
     3061 attaggcgcc gaagccttcg gcttcggtac ggcggcgctg gttgcgatcg gctgtgccat
     3121 ggcgcgccag tgccacctga acagctgccc caccggagtc gcaacccaac ggcctgacct
     3181 tcgggccaag ttcaagggca cgcccgaaca ggtgattacc tacttcacgc tgctcgcgga
     3241 ggaagtgcga ggaatcctcg catcgatggg attctggcgc ctggacgaga tcgtcgggcg
     3301 ccacgatctc ctcgaacgcg tcgagcgccc cgatatcccg cgggcgcaga tgctcgatct
     3361 ttccatgctg cttgaatcag cctcaagcgg gccgctgcgg cgaaccgaag accgcaatga
     3421 ccgtccggga atgacttgcc tcgatgatga ggttctcctg acgttgggcc cgtacctcga
     3481 cagcgggctt ccgttcgcgg gcaactacga gatcaggaat caccacctcg cagtaggagc
     3541 gcgagtggcc ggcgtcgtgg cggcgcgttt cggcaacgcc ggactccccg agggccgaat
     3601 ccgcttgcgc ttcagtggcg cggccgggca agtttcggcg cgttcatcac gcccggcatg
     3661 catttggatc tcgagggcga agccaacgac tacgtcggca agggcctcag cgggggcgag
     3721 atcaccattc ggccgtaccg aaaagccgca tacgccgatc aaacccacca gaatctcatc
     3781 atcggcaata ccgtgctcta cggtgcgacg gggggaaagc tgttcgcggc cggtcaggca
     3841 ggagaccggt ttgcagtgcg gaactccggc gcaatcgcag tggtggaagg cgccgggaat
     3901 cactgctgcg agtacatgac ctccggaatt gtcgtcgtgc tgggacgtgc cggccggaat
     3961 ctcggtgccg ggatgtccaa cggagtggcc tatgtgttgg atgagcctgg cacgctcctc
     4021 gagcgagtca acttagatat ggttgacgtc gtcgacctgg atgcggaaga cgaggacgtg
     4081 cttcgccgtc tggttaggga gcacgaagag aagaccacca gtccccgcgc ccgaaccatt
     4141 ctggtggagt gggaccaata tcggcccctg ttccgcaaga ttgctcccaa gggtgcggaa
     4201 gcgtacatgg ccgcaacgcg cgctgcgtac ctgcaaggct cgcagccgga gccggagctt
     4261 gccctcgctc ggcgaagcgc gtagcagtta gcttcagggc gccatgcttc ggcgtccttc
     4321 ccgtctcacc atcctgcttg gagtgctcct gggtgcagcg ccgtcagtgg cctgcggcca
     4381 gggcacttca tcgcccgatc gaaccatcct cggattcgag ctcggcttct cccgtgcaag
     4441 cttctcgccc gccctgggga ccgaggatcg cgagggcggc atcatcggcg gcttcatcgc
     4501 cgagcgcttc ggaaagctgt tcgccctgca gggggagctg ctcttcacga agaagggtgg
     4561 gtcggtaccg gtcgaaagtc agtttgggtt ggtcgacgtc ggcgtccagc tgatctacgt
     4621 cgaagctccg ctcctggctc gggtgactct gcccgtgggt acccacttga gaccagtggt
     4681 attcggtggc ggatcgtttg ccctgtccgt cggctgcgag ttccagttcg agattcaggg
     4741 cacgctcaac caggcccgct gcgaccagcc aggtacagga ctcgagctgt ccagcaccga
     4801 ctggagtggg attttcggcg gcggagtcga gtatggatgg cgccgatcgg cggtccgact
     4861 cgagcttcgc cgcttcattg gcctgaaaaa tctggtcgaa ggcgtcgaac aaaagaaccg
     4921 tgcctgggtc gttctcttcg gcatcaactt ctagcagcga acacagcgac caggccgaag
     4981 gcgaccagca tcaatcccga catccaattg atagctcgaa gcgccccaac cccgagcctt
     5041 ctgcctaccc aacctacgcc gctgctcaga agcaaccacc agaaggccga cccgagaaac
     5101 acggcggcca ccagagtcac gcccgcaaga tgtccccggc ccgccgtcgc gacgagaccc
     5161 aggccggcga acgccgctgc gaacgaaagg atcgtgagcg ggttggtgac ggtgagggca
     5221 agcgttgtca ggta
//
LOCUS       DASRJQ010000010         2962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1401481, whole genome shotgun sequence.
ACCESSION   DASRJQ010000010 DASRJQ010000000
VERSION     DASRJQ010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401481"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..718)
                     /locus_tag="VFB89_00290"
     CDS             complement(197..718)
                     /locus_tag="VFB89_00290"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25781.1"
                     /translation="MSLVLFDVLYVGGKSVMREPWRDRRKRLEDLIESLQLPRIAVVP
                     VTEDAPTLYEMWAGMGGEGIVLKDPASLYRPGERSSAWLKLKPKLTLDVTVTGGSAEP
                     IKWGDWGEAVMLELEYAHPRSGERLKIRQAVRIARQQPFELKVGARASVVCWGVMPSG
                     ILRHPLFLGWAAS"
     gene            complement(1303..1572)
                     /locus_tag="VFB89_00295"
     CDS             complement(1303..1572)
                     /locus_tag="VFB89_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25782.1"
                     /translation="MPRDSVIAAAASATQRAALEAIRRMAQPRRPSPRNRPPKELAID
                     IIASEIRSAQREVWLEASRLVLAPGELTEIARRMRERAERAIKPE"
     gene            1790..>2962
                     /locus_tag="VFB89_00300"
     CDS             1790..>2962
                     /locus_tag="VFB89_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZM25783.1"
                     /translation="MSQRARLRVGLLRWRALWWTDPRGSGERPGLRLSSKIFLASALV
                     ISVLAGVSALSLGAVGRLVSANREITTHTIPALALAASARDAISPLLRLEARALVLGD
                     RHYAKAWTELTEQVAEDLLRLADYPLSAPEAMHRREASTAFEEYRHIVAEEQTLLQRG
                     DRAGALRLRDAAARMRAEQVGESLDKLMTATRLRILAAQAEAARLEKVTWTVVLMALG
                     AAIGLALLGTAVVARRMTRSIDRLSTAMAEVAASAFREPIAVESHDEIGALARSFNWM
                     AGELRQMEETKQEFFATVSHELRSPLTSICGAVELLHRGAPGPLTESQARLTEIIGQS
                     SERLLRLTNHILELSRFRAGLVELDRKALDLAALVDRVVEELHPQAAEAGVAVERER"
BASE COUNT          500 a          965 c          965 g          532 t
ORIGIN      
        1 ctaccgtgag atcctcagcc ggatcgcggc ggaacttggg gtcacctacc tggatacgga
       61 cgcattcaca tcggccgatc ggtcactgtg gctccccggc gacggccact tttcggtcct
      121 cggcgcgcag cggatggccg cgctggtggc ggcgcacgtc gggcgctagc cgagcttgct
      181 cttcccgagc aatgcgctac gaggcagccc agccgagaaa cagaggatgg cgcaggatcc
      241 cactcggcat gacgccccag cagactaccg aggcccgcgc tcccaccttc aactcaaagg
      301 gctgctggcg ggcgatccgc acagcttggc gaatcttcag tcgctcgcca ctacgcgggt
      361 gggcatactc cagttccagc atcaccgcct cgccccaatc gccccacttg atcggctccg
      421 ctgagccgcc agtcacagtc acgtccagtg tgagcttggg tttgagcttg agccaggccg
      481 aggagcgttc acccggacgg tagagtgatg ccggatcctt caggacgatg ccctcgccac
      541 ccatgcccgc ccacatctca tacagggtcg gagcatcctc agtcaccggg accacagcaa
      601 tgcgcggcag ctggaggctc tctatcagat cctccaggcg cttccggcga tcacgccacg
      661 gctccctcat gacgctcttg ccgccaacgt agagcacgtc gaagagcacg agggacatgt
      721 cgccgccagg tcgcttccgc gcgctgaaga ctgcctgtat gccctcgtgg ccgtctcctg
      781 cacacgcctc cccatcgacg gaaggagggc atgccccatc tgcgcggcaa gcgctcttcg
      841 tgaggtgcta cgtgacgtcg gcctcgacgt cgagtctgcg agccacgccg accagtcgcc
      901 gacgaggtgc gcgagcagtt gctaggggtc cacggtgtga caaagaacgc gtgcgtaatt
      961 cggccggtcg cttggatgac gtgcgtgtgg gatgcccggc ggagcagcgc gtgttggagc
     1021 ttagttagtc gctagccata tgaaccccag cccaatgaaa acacctgtaa ctgctgcaag
     1081 gaagatcttg aggtcgagcg gcgttaaccg actgttgaag tcccggtagt gtcgcatcga
     1141 tgcctccgga tagattgcag ggatcttcgg tggtagaggc gagatcgtcg atcgggggaa
     1201 accttggtcg gggcgggaga aaacccgtat ttggttccgg aaatattgaa agtcactaaa
     1261 cagcggtgat caatcggcag ctcgcgggag ttaggaagtg acctactcag gcttgatggc
     1321 ccgctcggcc cgctcgcgca tcctgcgggc aatctcggtc agctcgcctg gcgccagcac
     1381 cagcctggat gcctcaagcc agacctcccg ctgcgccgac ctgatttcac tggcgatgat
     1441 gtcgatggcc agctccttgg ggggtcggtt tcgcggagac ggcctcctag gctgcgccat
     1501 ccgtctgatc gcctctaacg ccgcccgctg cgtcgcagac gcggctgcgg cgatcacgct
     1561 gtcgcgcggc atgcagggac cgcgctgcgc actccgaagg ttggctcgag agcatccgtc
     1621 cgcctaaaga cttaccagtt cgctctcctc cggaaataca gaaacttctg aatctcttgg
     1681 cattcatttg ccggtatcaa tcttgcccca aattcccgcc atggacgggt caggtcgaat
     1741 atcggaaact ccacgacacc cgcgcgttct atgtctggtg agccggctca tgagtcagcg
     1801 cgcccggttg cgggttgggc tacttcgctg gcgagcactc tggtggactg acccgcgcgg
     1861 aagcggcgag aggccgggcc tgcggctcag ctccaagatc tttctggcct ctgccctcgt
     1921 gatcagtgtg ctcgctggcg tgagcgcgtt gagcctcggc gcggtgggcc gtctcgtgtc
     1981 cgcgaaccgc gagatcacca cgcataccat ccccgcgctc gccctcgccg cgtccgcccg
     2041 ggatgcgatt tcgccacttt tgcggctcga ggcgcgcgca ctggtgttgg gggatcggca
     2101 ctatgcgaag gcctggaccg agctgacgga gcaggtggcc gaggacctcc tgcgccttgc
     2161 cgactatccg ttgagcgcgc ctgaggccat gcaccgccgc gaggccagca cagcgtttga
     2221 ggagtaccgg cacattgtcg ctgaggagca aacgcttctc cagcgcggcg accgcgccgg
     2281 cgccctgcgc ttgagggacg ccgccgcgag gatgcgcgct gagcaggtcg gggagagcct
     2341 cgacaagctg atgacggcga cccgcctgcg gatcctcgcg gcgcaggccg aggccgcgcg
     2401 cctcgagaag gtcacgtgga cggtagtgct gatggcgctc ggcgccgcga tcggcctagc
     2461 actgctgggg acggcggtcg ttgcccggcg aatgactcgt tccattgacc gtctatccac
     2521 ggcgatggcg gaggtcgccg cgagtgcgtt ccgggagccc attgccgttg aaagccatga
     2581 cgagattggg gctcttgccc gctcgttcaa ctggatggcg ggcgaactcc gtcagatgga
     2641 agagacgaag caggagttct tcgcgacggt ctcacacgag ctgcggtcgc cgctcacgtc
     2701 gatctgcggc gcggtcgaac tccttcaccg gggcgctccc gggccgttga cggagagtca
     2761 ggcgcgcctg acggagatca ttgggcagag ctccgagcgc ctgctccggc tcaccaatca
     2821 tattctcgag ctgtcgcgct ttcgcgccgg gctcgtggaa ctcgaccgca aggctctcga
     2881 cctcgcggcg ctcgtagacc gcgtggtcga ggagcttcac ccgcaggccg ccgaggcggg
     2941 ggtggccgtg gagcgcgagc gc
//
LOCUS       DASRJQ010000011         2111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_4209785, whole genome shotgun sequence.
ACCESSION   DASRJQ010000011 DASRJQ010000000
VERSION     DASRJQ010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4209785"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VFB89_00305"
     CDS             complement(<1..449)
                     /locus_tag="VFB89_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25784.1"
                     /translation="MLLSLPCGLTAQRPAESAMPAAPRAIREIREADLRRDLFAMASP
                     AMRGREGGTLDEMKASIWVAEQYRGIGLEPAGDDGTWFQWFNITRTRVSVTASHASIA
                     GQELSLFRDVIPLTVAPVEATGPVLWIADPQDTTADVRGRIVATLLRP"
     gene            655..2040
                     /locus_tag="VFB89_00310"
     CDS             655..2040
                     /locus_tag="VFB89_00310"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZM25785.1"
                     /translation="MSKLFSLPLSLTALILLGPNAAPAGAQSDRAALVRKLDSLAGSG
                     VLENRAVGIAAAVVRGSDTLLLKGYGKADVEWNVPLPVDAIFEIGSVTKQFTAAALLQ
                     LRDAGKLSLDDDITKWLPDFDTRGNTLTLRRLLDHTSGIKGLTEMPEFGNLAVNPRFP
                     RDSAYALIKRYPFEFKTGEAQIYNNSAFWLLGLVVEKASGGTYEDYVEKKIFEPLGMK
                     RSMYCNSSENVERRAHGYGVQNGIIRRAPVNVHTWPFSAGSLCSTVGDMVTWLKALHG
                     GKVLSPKSYAEMVAPSKLNDGTPLGYGMGIAVGKDIRGLKFIGHGGSIAGFTAEAAWY
                     SDAQAAVVVLMNSNGNIDPGAVAGELAAELLPWERPEVKQFSGDATPLLGTYKGPSRG
                     REMVVEVTQGAQGIEFSVNGSPKRSLPWVEGLTFRQGTALLTFRQMGSSGPATELRFQ
                     AGSAYYILKRQ"
BASE COUNT          366 a          682 c          707 g          356 t
ORIGIN      
        1 ggccggagca gcgtcgcgac gatgcgcccg cgcacgtcgg cggtggtgtc ctgagggtca
       61 gcgatccaga gcaccggccc cgtcgcctcc acgggtgcta cggtgagcgg gatgacgtct
      121 ctgaacaggg acagttcctg accggcgatc gacgcatggc tcgccgtcac cgaaacacgg
      181 gtgcgggtga tgttgaacca ctggaaccag gtgccgtcgt ctcccgcagg ctcgagaccg
      241 ataccccggt attgctcggc gacccagatg gaggccttca tctcgtcgag ggttccgcct
      301 tcgcgcccgc gcatggccgg ggaggccatg gcgaagagat cacgtcgcag gtcggcttcg
      361 cggatctctc ggatggcgcg cggagcagcc ggcatggccg actcggcggg gcgctgcgcg
      421 gtcagcccgc atggcagcga caacagcatg atgaggattg tccttcgggc ggcggagcat
      481 tgcctcatac acgcctctgg tcgatcgccg ggctcgagct ttcgctgaca ggtatgatcc
      541 gaacgtgaat atatcgcggg gccgggaagc gcgttcctcg cgctgaaacc ttgccaaggg
      601 tggaaacgta ggtgcacctt cttcctcaac caccgcctgc aaacccggaa gctcatgtcc
      661 aagcttttca gcctgccgtt atccctgacg gcgctcatcc tgctcgggcc caatgccgcg
      721 cccgccggtg cgcagtccga tcgtgctgcg ctcgtcagga agctcgactc gctcgccggc
      781 tcgggagtcc tggagaatcg cgccgtgggc atcgctgccg ccgtcgtcag gggcagcgac
      841 accctgctcc tcaagggcta cggcaaggcg gacgtcgaat ggaacgtccc gctgcccgtg
      901 gacgcaatct tcgagatcgg atccgtcacc aagcagttca ccgccgccgc actcctgcag
      961 ctccgcgacg caggaaagct gagcctcgat gacgacatca cgaagtggct ccccgacttc
     1021 gacactcgcg gcaacacgct cacgctccgg cggctcctgg atcatacctc cggcatcaag
     1081 ggtctcaccg agatgccgga gttcggcaac ctcgctgtca atccccggtt ccctcgcgat
     1141 tcggcctatg ccctcatcaa gcgctatccg ttcgagttca agaccggtga ggcgcagatc
     1201 tacaacaact cggcgttctg gctgcttggg ctggtggtcg agaaggcgag cggcgggacc
     1261 tacgaggact acgtcgaaaa gaagatcttc gagccgttgg gcatgaagcg cagcatgtat
     1321 tgcaacagct cggagaacgt ggagcgccgc gcccacggat acggcgtcca gaatggcatt
     1381 atccgccgcg cgccggtcaa cgtccacacc tggcccttct ccgcggggtc gctctgctcg
     1441 acggtgggcg acatggtcac ctggctcaag gctttgcacg gcgggaaggt gctctcgccg
     1501 aagtcatacg ccgagatggt cgcgccgtcg aagctgaatg acggtacgcc gctcgggtac
     1561 gggatgggga tcgcggtcgg aaaagacatc cgtggtctca aattcatcgg tcacggcggg
     1621 tcgatcgccg gtttcaccgc cgaggccgcg tggtattccg acgcgcaggc ggcggtggtc
     1681 gtcctgatga acagcaatgg gaacatcgat cccggcgcgg tggcgggcga gctcgccgcc
     1741 gaactgctgc cctgggagcg tccggaggta aagcagttct ccggcgacgc gaccccgctg
     1801 ctcgggacct acaaggggcc gtcgcgtgga cgtgagatgg tcgtggaggt gacccagggc
     1861 gcgcaaggca tcgagttctc ggtgaatgga tcacccaagc ggtcactccc gtgggtggag
     1921 gggctgacgt tccgccaggg gactgcgctc ctgaccttcc ggcagatggg aagcagcggt
     1981 ccggcgacgg agctgcgctt ccaggccgga agtgcctact acatcctcaa gcggcagtaa
     2041 gacctcacgg caccacccgg tggcgcgacg gccgctagcg ggtgcggcgc gctgcggcgt
     2101 cgacctcggc g
//
LOCUS       DASRJQ010000012         3321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1659449, whole genome shotgun sequence.
ACCESSION   DASRJQ010000012 DASRJQ010000000
VERSION     DASRJQ010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659449"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VFB89_00315"
     CDS             complement(<1..722)
                     /locus_tag="VFB89_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25786.1"
                     /translation="MVVVLVSLLAGLSLGTGWLIVRHFREDAESTSRFYSEVFAGLNS
                     PRPGAEAEALLRLGEQVRALGMPLVVTDAAGRVTAYDNLPFETSLDDPRLKQFVGGLD
                     RQNPPVVDSTIGTVHYGPLPARLQLTALVVLQALTIAVMVGVAIFAYRSAMNAQRDRL
                     WVAMAREAAHQMGTPLTSLQGWIERIRSLPEAPPALGEHLSADAERLDRVARRFERIG
                     NPAAREPIGLGALADRVAGYFRP"
     gene            complement(751..2034)
                     /gene="serS"
                     /locus_tag="VFB89_00320"
     CDS             complement(751..2034)
                     /gene="serS"
                     /locus_tag="VFB89_00320"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HZM25787.1"
                     /translation="MIDLKRLRQDPDAGRAGLARRLDPALPEQLDLVLELDRRHRDRL
                     ARFETCRADRKAASEEVARRKKNKEPADDLLARLKASGEEEKALEAQAREAESQLMHE
                     LSLLPNFLLDEVPEGGEAENCVVRTWGTPPAFSFVPKPHWELGEKLGILDLPAGARLT
                     GSGFPVLRAMGAKLVRSLANFMLDLHVREHGYVEVAPPYVVNYDSMFGTGQMPKFAEE
                     LYQVRDDGLFLIPTAEVPVTNIHRGAVLDGADLPLAYVAWTPCFRREAGAHGKDTRGL
                     IRVHQFDKVELVRFCRPEDSEAQHELITRHAEAVLQRLELPYRVVDLAAGDTGHTSAH
                     TFDLEVWAAGVGAWLEVSSASTFTDYQARRANIRYRPEPGAGPRFVHTLNASGVAFPR
                     CLIALLENGQQADGSIRLPDALVPYVGVSRIDPAS"
     gene            complement(2041..2601)
                     /locus_tag="VFB89_00325"
     CDS             complement(2041..2601)
                     /locus_tag="VFB89_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25788.1"
                     /translation="MRRILFVLGLLPLGFAAGGCNSNPLGDAQQPNVVDTIVLGALVG
                     TSIGTPSAFSIPASAAVRTDQTSQFDFAYNIESGSRHVFLPQGALGISNTSGVSPGLL
                     LSDQSFNEITEAPLNGYNTIDTITIAVNQVWVARSSLVCTSVGVPVYAKLRILSFDDV
                     NREVKFEVLANRNCGFRELTPGIPQN"
     gene            2721..3071
                     /locus_tag="VFB89_00330"
     CDS             2721..3071
                     /locus_tag="VFB89_00330"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HZM25789.1"
                     /translation="MSDLGEVVRAFGDRPDVAAVVLASADGLPIHTAGRHPVDADSTA
                     ALAATFVRQAAALSEATGLGQMETAVLESARGLAVVARVGADWLVVVPTESADAGALL
                     YHLRVHRPALTALL"
     gene            3120..>3321
                     /locus_tag="VFB89_00335"
     CDS             3120..>3321
                     /locus_tag="VFB89_00335"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZM25790.1"
                     /translation="MRWAVILAGGSGTRFWPLSTPDSPKQLLPLSGNRSTAREAVERL
                     EGLISRDRILIVTGAALAPKLQD"
BASE COUNT          577 a         1075 c         1094 g          575 t
ORIGIN      
        1 ggtcggaagt agcccgctac ccggtccgcc agggcgccca gcccgattgg ctcgcgagcg
       61 gccgggtttc caatccgctc gaagcgccga gccacccggt cgaggcgctc ggcatcggcc
      121 gagaggtgtt ctcccagggc gggcggcgcc tcgggcagtg accggatccg ctcgatccaa
      181 ccttgtagac tcgtgagtgg ggtccccatt tggtgcgctg cctcgcgggc catcgcgacc
      241 cagagccggt cccgctgcgc attcatcgcg cttcgatagg caaagatcgc cactccgacc
      301 atcaccgcga tggtaagcgc ctgcagaact acgagtgccg tgagctggag ccgggcgggt
      361 agcggaccgt agtggaccgt gccgatggtg gagtccacta cgggcgggtt ctggcgatcg
      421 agtccgccca cgaattgctt gagcctggga tcgtcgagcg aggtctcgaa aggaaggttg
      481 tcgtaggcgg tgacacgccc ggcggcgtcc gtgacgacga gcggcattcc cagagcacgc
      541 acctgttcgc ccagcctcag taacgcttcg gcctcagcgc cagggcgcgg actgttgagg
      601 ccggcgaata cttcggagta gaagcggctg gtcgattccg catcttcgcg gaagtgacgc
      661 acgataagcc agccggtacc gaggctcaga ccggccagga gcgacacgag cacgacgacc
      721 accgccggcg cgagacgctc gaggcgcggt ctacgacgcc gggtcgatcc gcgagacgcc
      781 gacatacggc accagcgcgt cgggcagccg aatggatccg tcggcctgct gcccgttctc
      841 gagcagtgcg atcaaacagc gcggaaacgc cacaccagac gcgttcaagg tgtggacgaa
      901 gcgcggcccg gcacccggct cggggcggta gcgaatattg gctcgacgag cctggtaatc
      961 agtgaaagtg ctggcgctgg agacctcgag ccatgcgccg acgcccgcag cccacacctc
     1021 gaggtcgaag gtgtgggcgc tggtgtggcc cgtatccccg gccgcaagat cgaccacccg
     1081 atacggaagc tccaggcgct gcagcacggc ttccgcatgc cgggtgatca gctcgtgctg
     1141 agcctcggaa tcctcggggc ggcagaatcg caccagctca accttgtcga actgatgcac
     1201 ccggatgagc cctcgcgtgt ccttgccgtg cgcaccggcc tcgcgtcgaa agcacggggt
     1261 ccacgctacg tacgcaagcg gaaggtctgc gccgtcgagg actgccccgc ggtggatgtt
     1321 ggtgaccggc acttcggcag tgggaatgag aaacagccca tcgtcccgca cctggtacaa
     1381 ctcctcggcg aacttgggca tttggcccgt gccgaacatc gagtcgtagt tcacgacgta
     1441 gggcggcgca acttccacat atccgtgctc tcgaacgtgg aggtccaaca tgaagttcgc
     1501 gagtgaccgg accagtttcg ctcccatcgc tcgcagaacg gggaagccgg accccgtcag
     1561 tcgcgcgcca gccgggaggt cgagtatgcc gagtttctcg cccagctccc aatgtggctt
     1621 gggtacaaat gagaaggccg gcggcgtgcc ccacgtacga accacgcagt tttcagcttc
     1681 cccgccctcg ggaacttcgt cgagcaggaa gttgggcagc aggctcagct catgcatgag
     1741 ctggctctcg gcctcgcggg cctgcgcctc gagcgctttc tcttcctcgc cggatgcctt
     1801 gagccgagcc agcagatcgt cggccggctc cttgttcttc ttccggcgag cgacctcctc
     1861 acttgccgcc ttgcgatcgg cccggcaggt ctcgaacctg gccagccggt cgcggtggcg
     1921 ccggtccagc tccaatacga gatcgagttg ctccggcagg gcggggtcga gccgccgcgc
     1981 aaggcctgcg cgcccagcgt caggatcttg ccggagccgc ttcaggtcga tcatcgccgc
     2041 ctagttttgc gggattcccg gagtgagctc gcgaaagccg cagttgcggt tggcgagcac
     2101 ctcgaacttg acttcgcggt tgacgtcgtc gaaggagaga atgcgaagct ttgcgtacac
     2161 cggcaccccg acgctggtac agaccaggct actccgggca acccacacct ggtttacggc
     2221 aatagtgatg gtgtcgatgg tgttgtaacc gttcagcgga gcttccgtga tctcgttgaa
     2281 tgactgatcg ctcaacaaga ggccgggcga cacgccgctc gtattggaga tgcccagcgc
     2341 gccctggggg agaaacacgt gccggctacc cgattcgatg ttgtaggcga agtcgaactg
     2401 ggaggtttga tcggtccgaa cggccgcgct ggccggaatc gagaacgcac tcggcgtgcc
     2461 tatggaagtg ccaaccagtg cgccgagcac aatcgtatcg accacgttcg gctgctgggc
     2521 gtcgcccagc ggattgctgt tgcagccgcc cgcggcgaag cccagcggga ggagtccgag
     2581 aacgaaaaga atgcggcgca tgagaccgga aggttaagcc gcgtgcagag aggggtcaac
     2641 ccatactcat tgcttgactt cgctctcatc gacccctacg ttgcgctcac cttttccgcc
     2701 ccaacagccg aggaagttgt ttgagcgatc tgggggaggt cgtacgggcg ttcggcgacc
     2761 gtcccgacgt cgccgcggtg gtgttggcga gcgcggacgg cttgccgatt cacaccgccg
     2821 gccgacatcc ggtcgacgcg gattcgaccg cagctctcgc ggcgaccttc gttcggcaag
     2881 ccgcggccct gagcgaggcc accggactcg gtcaaatgga aaccgcggta ctcgaatccg
     2941 cccgtggatt ggccgtggtg gcacgggttg gggccgactg gctcgtggtg gtgcccacgg
     3001 agtcggctga tgccggtgcg ctactctatc atctgcgagt tcatcgaccg gcgctcaccg
     3061 ctctcctctg acgtgatccc gattgtgcat tcgcgcattc gcccggcccg gctgctccga
     3121 tgagatgggc ggtgatactg gccggcggct cgggcacccg gttctggccg ctcagcactc
     3181 ccgatagtcc caaacagcta cttcctttga gcggcaatcg ttcgaccgcc cgggaagcgg
     3241 ttgagcgact cgaggggctg atatcccgcg accgcatact catcgtgacc ggcgccgctc
     3301 tggcccccaa gcttcaggat c
//
LOCUS       DASRJQ010000013         3123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1659317, whole genome shotgun sequence.
ACCESSION   DASRJQ010000013 DASRJQ010000000
VERSION     DASRJQ010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659317"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="VFB89_00340"
     CDS             <1..946
                     /locus_tag="VFB89_00340"
                     /inference="COORDINATES: protein motif:HMM:NF013883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_function: GO:0003997 - acyl-CoA oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZM25791.1"
                     /translation="SEGHEVPILDYLTHQRRLFPAIATAYAFDFALKSLVGRFVAAIE
                     SGHDADAEYRRVEADGAGLKALVTWHTIGGLQAARECCGGQGFLAINRIAQLRADTDV
                     TATFEGDNTVLLQLVAKGLLTDYRQQFHAAGARGILRYLGRRASARLARVNPVLARRT
                     DEDHLRDPDFQLGVLRYREERLLGSAARRLKHRIEAGADGFGALIECQDHLLALARAH
                     VERRTLEEFARGTDNAEPGLRPVLHRLRELFALSVLEREEGWLLAHGVFEGAKAEAVR
                     ALVNQSCRELRPHAVALVNAFGIPDEILGAPIAMAGAP"
     gene            946..1854
                     /locus_tag="VFB89_00345"
     CDS             946..1854
                     /locus_tag="VFB89_00345"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZM25792.1"
                     /translation="MDGGQPQNSTGARARLAVAALLFSTGGAAIKAAAFTGWQIACFR
                     SGIAALVLWLLIPGARRGWSWRNVVVGITYAATLVLFVLANRLTTSANTIFLQSTAPL
                     YVLLLAPLLLRERVRPGDIAFMAVVGAGLLLFFVGAEAPLVTAPDPGRGNLLAAGSGV
                     TWALTVCGLRWMGRDGAEHAAIGAVVVGNALAFVATLPRALPLAAHPAMDWVVIVYLG
                     VFQIGLAYALVTSALRRLQAFEASIILLIEPAFNPLWAWVVHREVPSPWALGGGALIL
                     GATALRTLLEARSRLTVSSDSGVAAS"
     gene            1851..2483
                     /locus_tag="VFB89_00350"
     CDS             1851..2483
                     /locus_tag="VFB89_00350"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HZM25793.1"
                     /translation="MRLGLIETVRVRDGVAPLWGLHLRRLLRSCRELGVPFPGEFDVP
                     SGGRNRVHRLEVSARGVEVTERAVEAVDAVRLATSSVVHHPYPHKTTDRGQFDRAIRG
                     AHEAGADDALLLAEGGWVAEAGIWSVFWWEGEQLCAPPFSLGILAGVARGRIAELTNI
                     EERRVPRAGLNGHPLLVANAVRGIVEVVSLDGVPTPRSDATRVLSARFWP"
     gene            2545..2618
                     /locus_tag="VFB89_00355"
     tRNA            2545..2618
                     /locus_tag="VFB89_00355"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2579..2581,aa:His,seq:gtg)
     gene            2691..2763
                     /locus_tag="VFB89_00360"
     tRNA            2691..2763
                     /locus_tag="VFB89_00360"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2724..2726,aa:Trp,seq:cca)
     gene            2894..2966
                     /locus_tag="VFB89_00365"
     tRNA            2894..2966
                     /locus_tag="VFB89_00365"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2928..2930,aa:Glu,seq:ctc)
     gene            3007..3078
                     /locus_tag="VFB89_00370"
     tRNA            3007..3078
                     /locus_tag="VFB89_00370"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3040..3042,aa:Glu,seq:ttc)
BASE COUNT          470 a          911 c         1097 g          645 t
ORIGIN      
        1 gtccgaaggg cacgaggtgc cgatcctgga ttacctcacc caccaacgac gactgttccc
       61 tgcgatcgcc accgcgtatg cgttcgactt tgcgctcaag agtctggttg gccgtttcgt
      121 cgcggcaatt gaaagcggcc acgatgccga cgccgaatat cgccgggtgg aggcggacgg
      181 agccggcctc aaggcgctcg ttacctggca taccatcgga gggcttcagg cggcccgaga
      241 gtgttgcggc ggtcagggat ttctggcgat caatcgtatc gctcaactgc gggccgatac
      301 cgatgtcacc gcgaccttcg aaggcgacaa taccgtactg ctccagctgg ttgccaaagg
      361 gttgctgacc gattatcggc aacaattcca tgcggctggg gctcgcggga tcctgcggta
      421 tctgggccgc cgggcatcgg cccggctggc acgcgtgaat ccggtgctgg cacggcggac
      481 ggacgaagat catttgcgtg atccggactt tcagcttggc gtgctccgtt atcgagagga
      541 gcgactcctc ggctcggccg cccgccggct caagcatcgt atcgaggccg gcgcggatgg
      601 ttttggcgcg ttgatcgaat gccaggatca cctgcttgcc ttggcccgag cacacgtcga
      661 acggcggacg ctggaagagt tcgctcgcgg gaccgataat gccgaaccgg gactccgccc
      721 ggtgctgcac agactgcgcg agctgttcgc gctttccgtc ctggagcggg aggagggctg
      781 gctcctggct catggcgtgt tcgaaggcgc aaaggccgaa gctgttcggg cgttggtgaa
      841 ccagagctgc cgggagctcc gaccgcatgc cgtcgcgctg gtgaatgcgt ttggaattcc
      901 ggacgagatc ctcggcgcgc ccatcgcgat ggctggagcg ccctagtgga cgggggtcag
      961 ccgcagaact ccacgggtgc gcgcgccagg ctcgcagttg cggcgctgct cttctccacc
     1021 gggggcgctg cgatcaaggc ggcggcgttc accggctggc agattgcctg cttccggtcg
     1081 ggcatcgcgg cgttggtgct gtggctgctg atccccgggg cgcggcgagg ctggagctgg
     1141 cgtaatgtgg tcgtgggcat cacgtacgcc gcgaccctgg ttctgttcgt gctcgccaac
     1201 cggctcacta cctccgcaaa cacgattttc ctgcaatcga ctgctcccct ctacgttctg
     1261 ctcctggctc cgttgctact gcgtgaacgg gtgcggccag gcgatatcgc cttcatggca
     1321 gtggttgggg ccgggctgct gctcttcttc gttggagcgg aagccccgct ggtaacagcc
     1381 ccagacccgg gaagaggcaa cctcctcgcg gcggggagcg gggtgacctg ggccctcacg
     1441 gtctgtggtc tccgctggat gggccgagac ggcgcggagc acgctgcgat cggcgccgtg
     1501 gttgttggca atgcgctcgc gttcgtggcg acgttgccgc gcgcgttgcc gctggcggct
     1561 caccccgcca tggattgggt agtcatcgtc tatctggggg tatttcagat tggtctggcg
     1621 tatgccctcg ttacttcggc actgcggcgg ctgcaggcat tcgaggcgtc gatcattctg
     1681 ctgatcgaac cggcctttaa tccgttatgg gcctgggtag tgcatcggga agtcccgagt
     1741 ccgtgggctc tcggaggagg tgcgttgatt ctgggggcga ccgctctcag gacgttgctc
     1801 gaagcgagat cccgtctgac cgtatcatcg gactcggggg ttgcggcctc atgaggctgg
     1861 gcctgatcga aaccgtgcgg gtgcgtgatg gcgtggcccc actctggggc ctacatctca
     1921 gacggctgct ccgaagttgc cgcgagttgg gagttccgtt tcccggcgag ttcgatgtgc
     1981 cgagcggggg tcgcaaccgg gtccatcgcc tggaggtcag tgcgagaggt gtggaggtga
     2041 ccgagcgggc ggtcgaagcg gttgacgccg tgcgcctcgc gaccagctcg gtggtgcacc
     2101 acccgtatcc gcacaagaca accgatcgtg gacagttcga tcgcgccatc cgcggggcgc
     2161 atgaagcggg cgccgacgat gcactcttgc tcgccgaggg agggtgggtt gcggaggccg
     2221 ggatctggag cgtgttctgg tgggagggtg agcaactttg tgcccctccg ttttcacttg
     2281 ggatactggc cggcgtcgcg cgaggtcgca tcgccgagct gacgaacatc gaagagcgac
     2341 gggtgccgag ggccggtttg aacggacatc cgctgctggt ggcgaatgcg gtgcgcggaa
     2401 tcgtggaggt ggtttcgctg gatggagttc cgacaccgcg ctcagacgcg acacgcgtgc
     2461 taagcgcccg tttctggcct tgacccgccc cgagcccggc gcgtattttc cgcggctcgg
     2521 aaatcggggc cagcttcctt cgtggtggtt gtagctcaat cggttagagc gccggactgt
     2581 ggctccggag gttgcgggtt cgattcccgt cagccaccct ggctcgagtg atccgcggag
     2641 cggtctgtcg ttccgtggtc ttttgcgtcg gagccgattg cctggtgcgt aggtccgtag
     2701 ctcaattggt agagcaccgg tctccaaaac cgggggttgg gggttcgatt ccctccgggc
     2761 ctgctttacg aactatcagc tatcgcttat cggctatcag tcgggggcga tgggccgcga
     2821 aactggatgg cgggcgtacg agtttgcttg atagctgata gcagatagct gatagctgat
     2881 agcttggttg cctggcgcta tcgtctaggg gactaggacg gggccctctc aaggcccaaa
     2941 cacgggttcg aatcccgtta gcgctatttc gagcagcaac gttgagcctg gctcgacggg
     3001 agtcccggcc ccatcgtcta tcggtcagga cgcgaccctt tcaaggtcga aagacgggtt
     3061 cgattcccgt tggggctatg tcggcggtcg cgttaggtcc gcgtagttgt taggcagtga
     3121 acg
//
LOCUS       DASRJQ010000014         3212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1965863, whole genome shotgun sequence.
ACCESSION   DASRJQ010000014 DASRJQ010000000
VERSION     DASRJQ010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1965863"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..1707
                     /gene="yidC"
                     /locus_tag="VFB89_00375"
     CDS             22..1707
                     /gene="yidC"
                     /locus_tag="VFB89_00375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HZM25794.1"
                     /translation="MDRRAIWAIVLMMAIALAPALFIKQGHRPAGRTASDSTTTTPVP
                     PPPTQAPAAAPAGPPAGTAKALAEDTVLVTSPLYTYGISTRGARLIRATLHRYPSMAA
                     STRGRPADLIRPGTGLLGATLVRGNDSLKLTDVIFTPSNSKLDVAAPSPLRLTAQHGD
                     VSVELTYQFAPDDYRIGVSGRVTGVGPNGGLLLLDLGPGLANTEADSNENHRALAIVT
                     KQGDAERTDFASLKPGEPKTLSGPFEWVAVKSKYFVTALLAFDSTGGSISGATAAAAA
                     TAPKHPTAAEIRLSLPVGPAGVFAFSLYAGPMEYDRLRQIGHDFDDVNPYGRPGFRTL
                     IRFFTVPIRWLLVFMHQELGLAYGIALVIFGVLVRLLLWPLNQKAMRSSMQMQAIQPL
                     MKELQERYKDDPTKLQTATMQLYKEHGVNPFGGCWPMLLPMPILLALFFVFQNTIELR
                     GASFLWLPDLSRPDPLYIIPLLMGLTMYGVSKVGQIGMEQTPQMRSMLYIMPVMMTVL
                     FLNFASGLNLYYAVQNLTSIPQQWMLAKERLKRQPPPPPPPPKVSKAKARSEK"
     gene            1708..3036
                     /gene="mnmE"
                     /locus_tag="VFB89_00380"
     CDS             1708..3036
                     /gene="mnmE"
                     /locus_tag="VFB89_00380"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HZM25795.1"
                     /translation="MLSDVIVALATPPGRSALAVIRLSGRAAFDVARQVVDGFEITPP
                     RQARLATFRSAQGQAIDRGLYTVFPGPRSYTGEDLVELSCHGGLAVPTQVIVALTAAG
                     ARAAHPGEFTRRAVLNGKLDLIQAEAVGDLIDATAPAQAGAALAQLEGGLSRRLAELR
                     TQLIELQALLGYDIDFPGEDDGPVPAARIARALDDASCRVVQLLATAPSGQRLREGAL
                     VVFAGRPNTGKSSLFNALLGVDRALVTEIPGTTRDAIEAHTAFLGWPVRLADTAGLQN
                     AVDRVERMGIEVSHRYVGSADLVLLCAEAGRRLGSDERAILEQRPAVLVRTKADLSNK
                     MPDGGLRVSSVTGEGLGHLRQAVAERIFGDRISLADLEPALTRERHQLALSRAREALA
                     DAQPHLGTGGDAVLVSHHVRRATLALDELVGVIDVEEVLASVFASFCVGK"
BASE COUNT          555 a         1032 c         1021 g          604 t
ORIGIN      
        1 gtgactcggt gaactagctc gatggatcgc cgtgccatct gggccatcgt tctcatgatg
       61 gccatcgcac tcgcgcctgc gctgttcata aagcagggcc atcggcctgc cggccggacg
      121 gcaagcgact ccaccacaac gacaccggtc cctccgccac cgacgcaggc gcccgccgcg
      181 gcgcccgctg ggccacccgc tggtactgcg aaggctctgg cggaagacac ggtactcgtc
      241 acctcaccac tctacaccta cggaatcagc acccgagggg cccgactgat ccgggccacc
      301 ctccaccgct atccctcgat ggccgcgtct actcgagggc gcccggctga tctgattcgc
      361 cccggcaccg gcttgctggg cgctaccctc gtgcggggca atgacagcct caagctgacc
      421 gacgtgatct tcacgcccag caactcgaag ctggacgttg ccgcgccgag tccactccgg
      481 ctcaccgctc agcacggcga cgtcagtgtc gagttgacgt atcagtttgc tcccgacgat
      541 taccggatcg gtgtctcagg tcgggtcact ggagtcgggc ctaacggggg ccttctcctc
      601 ctcgatctcg ggccaggcct ggccaacacg gaggccgatt ccaacgagaa tcatcgagcc
      661 ctggccatcg tgaccaagca gggcgatgcg gaacggaccg attttgcgag cctcaagcca
      721 ggcgagccaa agacactcag cgggccgttc gagtgggttg ccgtcaagtc caagtacttc
      781 gtcaccgcgt tgctggcgtt tgacagcacc ggcggaagta tcagcggcgc cacggcggct
      841 gctgcggcca ctgcccccaa gcacccgacg gcggccgaga tacggctcag cttgccggtt
      901 gggccggcag gggtattcgc cttctcgcta tatgcgggtc cgatggagta cgaccggttg
      961 cgtcagatcg ggcacgattt cgacgacgtc aatccttacg gccggcctgg attccgtaca
     1021 ctgatccggt tctttacggt accgatccgt tggcttttgg tcttcatgca tcaggagctg
     1081 ggtcttgcct acggaatcgc gctcgtgatc ttcggcgtgc tggttcggct gcttctctgg
     1141 ccgttgaacc agaaggcgat gcggtccagc atgcagatgc aggcgatcca gcccctgatg
     1201 aaagaattgc aggagcgcta caaggacgac cccaccaaac tgcagaccgc aacgatgcag
     1261 ctctacaagg agcacggggt caatccgttc ggcggctgct ggccgatgct gctgccgatg
     1321 ccgatcctgc tggcgctgtt ctttgtcttc cagaacacga tcgagctccg gggggcctcg
     1381 ttcctctggc tgccggacct ctcgcgcccg gacccgctct acatcattcc gctactgatg
     1441 gggctcacga tgtacggtgt gagcaaggtg ggtcagatcg ggatggaaca gaccccgcag
     1501 atgcgtagta tgctctacat catgccggta atgatgaccg tgctcttcct caacttcgcg
     1561 tcgggcctca acctctacta tgcggtccag aatctgacca gcatcccgca gcagtggatg
     1621 ctcgccaaag agcggctgaa gcgccaaccg cctcccccac ctccaccccc gaaggtgagc
     1681 aaggccaagg cacgaagcga gaagtagatg ctttccgacg tcatcgtggc cctcgctact
     1741 ccgcccggcc gctcagccct tgcagtaatc cggctcagcg gccgtgccgc gttcgacgtg
     1801 gcgcggcagg tcgttgacgg cttcgagatc acgcctccgc gtcaggccag attggcaacc
     1861 ttcaggagtg cgcaagggca ggcgatcgac cgcggcctct acacggtctt tccgggtccc
     1921 cggagctaca ccggcgaaga cctggtcgag ctatcctgcc atggcggtct ggcggttcct
     1981 acccaggtga tcgttgcatt gacggcggcc ggagcccggg cggcccatcc cggcgaattc
     2041 actcggcgtg ccgtgctgaa tgggaaattg gatctgatac aggctgaagc agttggcgac
     2101 ctgatcgatg cgacggcgcc ggcacaggcc ggcgcagcgc tcgcgcagct cgaaggtgga
     2161 ttgtctcgaa gactggctga gctccgcacg caattgatcg agctgcaagc gcttctcggc
     2221 tatgacatcg acttcccggg cgaagacgac ggacccgtgc ctgctgcccg gattgcgcgt
     2281 gcgctcgacg atgcgtcttg tcgggtggtg cagctcctgg cgacggcacc atcgggacaa
     2341 aggctcaggg aaggggcctt ggtggtgttc gcgggtcgcc cgaacaccgg caagtcctca
     2401 ctcttcaacg cattgctcgg cgtcgaccgg gcgctggtga cggaaattcc cgggacgacg
     2461 cgtgatgcga tcgaggctca caccgcattt ctcggctggc ctgtccgcct ggccgacacg
     2521 gccggacttc agaacgcggt cgatcgagtc gagcgcatgg gcatcgaggt gagccatcgc
     2581 tatgtcggct ctgcagacct ggtgctgctc tgtgcggagg ccggccgacg gctcggatcc
     2641 gatgagcgtg cgatactgga gcagcggccg gcggttctgg ttcgaaccaa ggcggacctt
     2701 tcgaacaaaa tgcccgatgg cgggctccgg gtgtcgtccg ttaccggtga aggtctggga
     2761 cacctgaggc aggccgtcgc ggagcggatt tttggtgacc gaatttcttt agcagatctc
     2821 gagccggcat tgaccaggga gcgccaccaa ctggcgctct cgcgggccag ggaggccctg
     2881 gcggacgcac aacctcacct cggcacaggt ggcgacgcgg tcctggtgtc gcaccacgtt
     2941 cgccgagcga cgctcgcgct ggatgagctg gtcggggtaa tcgacgtgga agaagtgctg
     3001 gcgagcgtgt tcgcgagctt ttgcgtcggg aagtagtttc gccaactacc gcttgtggct
     3061 tccgtcgcag aacggcttgg tctttgaaag gccgcagccg cacaacttga cctgccccgg
     3121 gaccttggtg ggcggtggga tcaactcccg accttcgttg tcacgtactg tgaccggtcc
     3181 ttcgatgacg taagggccgg tcggcttcca gg
//
LOCUS       DASRJQ010000015         2039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_562742, whole genome shotgun sequence.
ACCESSION   DASRJQ010000015 DASRJQ010000000
VERSION     DASRJQ010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562742"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(337..1287)
                     /locus_tag="VFB89_00385"
     CDS             complement(337..1287)
                     /locus_tag="VFB89_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HZM25796.1"
                     /translation="MKYRQLASEERYLISAFRIEGFNQAQIATRLGRAPSTVCRELAR
                     NHCPADGRYRPSKAHDRARWRRSRARRNQRFGAPDLALVERWLREDWSPEQVAGRLGE
                     DGVLSISHETIYRHVWADKRRGGDLFRHLRGAQKQRRKRYGAYDSRGRLAGKRSLAER
                     PACVETRQQLGHWEIDTVMGAGAQDCIVTAVERKTGYAVIGKLPERTTDALNRRTIQL
                     IRRHEDCFHTITADNGTEFHAYARIEAATGARFYFAAPYHSWERGSNENLNGLVRQYL
                     PKRVSMAGLTQQHCNAIAHKLNRRPRKRLGYKTPEECFYG"
     gene            1570..>2039
                     /locus_tag="VFB89_00390"
     CDS             1570..>2039
                     /locus_tag="VFB89_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25797.1"
                     /translation="MSLSWPPNPPRRLALGSAGLLLAISAALLLYPSVVSPYPADASS
                     EGGDPGPLLDILYALAALFATVRPATWNTLLTRTTTIEWRDAGLFRSPPNSRLSFRPG
                     ALIAGLLLLGCFVNALASPVLAISLLAQGQASWHLLAQVALRPLVAWTLAARVA"
BASE COUNT          239 a          735 c          659 g          406 t
ORIGIN      
        1 cacccgtcgc gcccctcgta cctcccggcg ccggccccgg cccttcgctc cgccatccct
       61 ttcggtttca atgcatggct ctgccctgcc cgacacgcgg cgcgcccgct tcgaccacgg
      121 gctcggcgga cgggccttgt ctggagggca tcgctcgagc cgagggccgg cgcgccgctt
      181 cttctcgtct ttcggggaag cctgcgagct tccagaggcg tacgggctcg gggcaggagc
      241 ccgctgcgcg cgcgggcgct ccctctcggc ctgcggcggc gcggtgcggc tctgggattg
      301 cggcctagct tcaagtttga agcgcaacgc tggtcctcag ccatagaagc actcctccgg
      361 ggtcttgtat cccagtctct ttcgtggcct tcggttgagc ttgtgggcga ttgcgttgca
      421 gtgctgctgg gtgaggcctg ccatgctgac tcgcttgggt aggtactggc ggaccaggcc
      481 gttgaggttc tcgttgctgc cgcgctccca cgagtggtag ggggccgcga agtagaagcg
      541 agctccggtc gccgcctcga tcctcgcgta cgcatgaaac tccgtgccgt tgtcggcggt
      601 gatcgtgtga aagcagtctt cgtggcggcg gatcagctgg atcgtgcgtc ggttgagcgc
      661 gtcggtcgtg cgctccggca gcttcccgat caccgcgtag cccgtcttgc gctccaccgc
      721 cgtcacgatg cagtcctgcg cgcctgctcc catcaccgtg tcgatctccc agtgtccgag
      781 ctgctgccgg gtctcgacgc acgcggggcg ctccgcgagc gatcgcttgc ccgccagacg
      841 cccccggctg tcgtaggccc cgtagcgctt gcgccgctgc ttctgggccc cgcgcaggtg
      901 ccggaacaga tcgcctccgc gccgtttgtc tgcccacacg tgccggtaga tcgtctcgtg
      961 gctgatcgag agcactccgt cctcgcccag ccgcccggcg acctgctccg ggctccagtc
     1021 ttctcggagc cagcgctcca cgagcgccag gtccggggcg ccgaagcgtt ggttgcgccg
     1081 tgccctcgag cgcctccaac gagcccgatc gtgggccttg ctcgggcggt agcgaccatc
     1141 cgccgggcaa tggttgcggg ccagctcccg gcagaccgtg ctcggcgccc ggcccagccg
     1201 ggtcgcgatc tgcgcctggt tgaatccctc gatccggaag gccgagatca gatatcgttc
     1261 ctctgaggcg agctgccggt acttcatccg cgattccccc ggtttggcga ttgggagcat
     1321 cgcaaaggac cggcactcgc ctcctctcgg gcttcgttcg ttgcacttag tttatgaatc
     1381 cgcgctgcct aacaaggcgc tgcagctgac cgaccgccgc cggtcgcttt gcccaaccct
     1441 ccagccggcc ggccgcccgg gtccggggtc gactcgccgg gcgtccgccg ggcggttcct
     1501 ggtacactca ccggcggccg gcagctgagc gccggtccgt taggcggcgg tcagaacccg
     1561 gcggcatcta tgtccctcag ttggcccccg aatcctcctc gtcgcctggc tctgggctct
     1621 gctgggctcc ttcttgccat ctccgctgca ctgcttctct acccctcggt ggtcagtccg
     1681 tatccagcgg acgccagctc ggaaggtggt gatccggggc ccttgctcga cattctctac
     1741 gcgttagcgg ccctgttcgc gacagtgcga cccgcgactt ggaatacgtt gctcaccagg
     1801 accacgacga tcgagtggcg ggatgcgggg ctcttccgaa gtccaccaaa tagccgcctc
     1861 tccttccgtc cgggtgccct aatcgcggga cttctcctct tgggctgctt cgtcaacgct
     1921 ctggcctcgc cagtgttggc gatctccttg ctcgcccagg gacaggcctc ctggcacctg
     1981 ctggcccaag tggcgctgcg cccgctcgtg gcgtggaccc ttgcggcgcg ggtggcaca
//
LOCUS       DASRJQ010000016         4634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3377075, whole genome shotgun sequence.
ACCESSION   DASRJQ010000016 DASRJQ010000000
VERSION     DASRJQ010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3377075"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VFB89_00395"
     CDS             <1..598
                     /locus_tag="VFB89_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25798.1"
                     /translation="RRLNSLFSADTIAGEPAKDPTRWDIATRLLELKAPGAESRYAEQ
                     VKRDTTADGRRHAFIAAAGRFDSTTKRTYFTRYFGDATLNEDWASGSLGEFNALEHQA
                     LTFPYLRPALDSLPYIQAHRRIFYLETWLAAFLRSQTGDSALAVVQHYLEEHPNLPID
                     LRRKVLQHADELERTVRIRHTEQPTPAVDSAGPRSTRG"
     gene            620..1249
                     /locus_tag="VFB89_00400"
     CDS             620..1249
                     /locus_tag="VFB89_00400"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HZM25799.1"
                     /translation="MSTGKLTLTTFLTLDGVMQAPGGPNEDPSGNFHHGGWIVPYVNE
                     DMLRIVGQRFGNADAFLLGRKTYQIFANHWPRVTDPADPIASALNRLPKYVASKTLEK
                     ADWNNSTVIRGDLANQVARLKQRYSREIQVHGSGDLAQSLIANDLVDEFHLWLYPLML
                     GTGKRLFGAGTVPAALTMAETQTTSTGVVVSIYRRSGKPTYGSFELDQN"
     gene            complement(1410..2849)
                     /locus_tag="VFB89_00405"
     CDS             complement(1410..2849)
                     /locus_tag="VFB89_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25800.1"
                     /translation="MTGLPHLKIALFSLTTLLALTACEQAGETPTAPAAPLGRIQRQQ
                     GAYDLSSLSPSLFVGGRRPAERRAPLLFQRAPFESQSAKKKPKGKPSPLANGSFELNG
                     GLSTNQFTDWTVFDTTVFSGSWLVQQGSGSPLNGFTVGSPTDGDFAAMTDQFGPGLHI
                     LYQDVQVPPHGQAILSFDLYLNNLAGAFFTPGTLSPDEFPNQQFRVDIVDPSAPLDDV
                     AGVLLPVYRTEEGDSATVPYRNVSVSLKQFAGRTVRIRFAEVDNQFFFLVGLDRVQLG
                     HKPRSLKAPKVAGRATANAIPFAPEAGPFPNVLTLDDDQTTGPLPIGFEFEFFGVRYS
                     QFNVSSNGFVSFDADAPNGCCGGGVIPSDDGLNNLIALAWTDLYPPGGGQIAYAVRGT
                     APNRRLVVTFTDISWCCEIGVPRLATQLILRERKGVIEIHTTHQDPGHIYTQGVENSL
                     GTVAAFLPGRVAADYGLDQDGVRFTTSAH"
     gene            complement(2949..>4634)
                     /locus_tag="VFB89_00410"
     CDS             complement(2949..>4634)
                     /locus_tag="VFB89_00410"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM25801.1"
                     /translation="AGTLWQARVAAHEATKEREVRRVLVDLFRGSSPQESRGRDVTAR
                     ELLERGARRLDTALAGHPDVHAELLGVLGVIHRDLGLYPRADSLLGRAVTLTRALRGG
                     SAPEVAERLTDWATVLTEEARYDEADSLLRQALAIRRSKRGPDDSTVAKTLRELGSVQ
                     RRKANYAQAEALHREALAIDRAHSGAVSLAVAEDLNELGVVLVEAKKFAAADSANREA
                     LAIRRKLLDAGHPDVLVSLHNLASVRQAVGDYGEAERLYREVLQERRRLYPQGHPLMA
                     DDLHELALTLQGAGRMPEAESLLRETLSMRRSLLGPDHPETIGAVNSLAVIHYFMGNL
                     AGAEENMREVLANWRRTLGENHRNTLFALNNLGAILREEGEYAEAEAVLRDALARRRR
                     LLGDADQDIAQSLQNLGLLLRYKGELAEAERALTEALAMDRKVLPPGHPLTLGILMDL
                     GGIKTDKGSARDAEPLLRESLTGWLAKAGPDDNRTQGARRELGACLTRLGKYQEAETV
                     LLEAYHNLAARTDFWGGKRRGQVVNLLVALYQRWGKRADAAKYRALADAHPAT"
BASE COUNT          813 a         1552 c         1448 g          821 t
ORIGIN      
        1 tcgcagactg aatagcctct tctcagcaga taccatcgcg ggcgagccgg ccaaggaccc
       61 gactcggtgg gacatcgcta cccgtctgct cgagctcaaa gcgcccgggg cggagtcgcg
      121 ctacgccgag caggtgaagc gtgacactac ggccgacggg cgccgccacg cgttcatcgc
      181 cgccgccggc aggttcgact cgaccaccaa gcgcacctat ttcacccgct atttcgggga
      241 cgccacgctc aacgaggact gggcctcggg cagcctgggc gagttcaatg cactcgagca
      301 ccaggccctc acctttccgt atttgcgtcc ggctctcgac tccctgccct acatccaggc
      361 gcaccgccgg atcttctacc tcgaaacctg gctggccgcc ttcctccgca gccagaccgg
      421 ggactcggcg ctggcggtgg tgcagcacta tctcgaggaa catccaaatc ttccgatcga
      481 cctccgccgc aaggtgctcc agcatgccga cgagctggaa cggacggtgc gaatccggca
      541 tactgagcag ccgacccctg ctgtcgattc agcggggcct cgatcgacga gggggtagac
      601 cacagccacg gagcgaatga tgtcgaccgg caaactcacg ctgaccacct tcctcaccct
      661 cgatggcgtc atgcaggcgc ccggcggacc caacgaagac ccgagcggca acttccatca
      721 cggaggctgg atcgtcccct acgtcaacga agacatgctc aggatcgtag gccagcggtt
      781 cggcaacgcc gatgcgtttc tgctcgggcg gaaaacctat cagatctttg cgaaccattg
      841 gccgcgtgtc accgatcccg ccgacccgat cgcctcggcg ctgaaccggc ttcccaagta
      901 cgtggcgtcg aagaccctgg aaaaggctga ctggaacaat tccacagtca ttcgcggcga
      961 tctcgccaac caggtcgccc ggctcaagca acgctactcg cgggaaatcc aggtgcacgg
     1021 cagcggcgac ctggcccaga gcctgatcgc gaacgatctt gtggatgaat tccacttgtg
     1081 gctctacccg ctgatgctgg gcacgggcaa gcgcctcttc ggggctggta ccgttccggc
     1141 cgcgcttacg atggcggaaa cccagacgac gagcaccggc gtagttgtca gcatttatcg
     1201 ccggtccggc aagccgacct acgggtcgtt cgagctggat cagaattgag cgcccccgcc
     1261 tcgagcggca cttccgcgac gcccagatcc tgcggcccca cggcttcacc tcagagaatc
     1321 gcttcgaggc tgcgggccag gtctacctcg gcgtgccgcc cgagtttgcc atggtggcgt
     1381 tttgagtatc ggtcggcgca cgcccttccc taatgggcgg aggtggtgaa gcgaacgccg
     1441 tcctggtcca gcccatagtc ggccgccact ctcccgggca agaacgccgc cactgtaccc
     1501 aggctgtttt cgactccctg ggtgtagatg tgccccggat cctggtgagt cgtatgaatc
     1561 tcgatcactc ccttgcgctc gcgcaggatc agctgcgtcg ccaaccgcgg tacgccgatc
     1621 tcgcagcacc acgagatgtc ggtgaacgtg accaccaatc gccggttggg agcggttccc
     1681 cggaccgcgt acgcgatctg tccgccgccg ggcgggtaga gatcggtcca ggcaagcgcg
     1741 atcaggttgt ttagtccgtc gtcggacgga atcactcctc caccgcaaca gccgttgggt
     1801 gcgtccgcat cgaagctgac gaagccattc gatgatacgt tgaactggct atagcggacg
     1861 ccgaagaact cgaactcgaa tccgatagga agcgggccgg tcgtctgatc gtcatctagt
     1921 gtcaggacgt tcgggaacgg ccctgcttcg ggcgcgaatg gaatcgcatt ggcggtggca
     1981 cgtccagcca ccttcggtgc cttgagcgac cggggcttgt gacccagctg cacccggtcc
     2041 aggcccacga ggaagaagaa ctggttgtcc acctctgcga aacggatccg cacggtgcga
     2101 ccggcgaact gcttgaggga gacgctgaca ttcctatagg ggacggtcgc agaatcgcct
     2161 tcttcagtcc ggtacaccgg caatagcaca cccgcaacgt cgtcgagtgg cgccgacgga
     2221 tccacgatat ccacccggaa ttgctggttg gggaactcgt cgggagacag cgtaccaggt
     2281 gtgaagaacg ctccggccag gttgttgagg tagaggtcga agctcaggat cgcctgaccg
     2341 tgcggcggga cctgcacatc ctgatagaga atgtggaggc ccggtccgaa ctggtcggtc
     2401 atcgcggcga agtcgccgtc ggtcgggctt cccaccgtga agccgttcaa cggagagccg
     2461 ctcccctgct ggaccaacca gctccccgaa aagacggtgg tatcgaacac ggtccagtca
     2521 gtgaactggt tggtgctcag tcccccgttc agctcgaagc tgccattggc gagcggggat
     2581 ggcttcccct tgggcttctt ctttgccgat tgggactcaa acggagcacg ctgaaataaa
     2641 agcggggccc tccgttcggc agggcggcgg ccgccgacga acagcgaggg cgacagggag
     2701 ctcaagtcat atgcgccctg ctggcgttgg atccggccga gaggagccgc aggagctgta
     2761 ggcgtctcgc ctgcctgctc gcaggctgtc agggcaagga gtgtcgtgag cgaaaacagc
     2821 gcgattttca agtgcggcag gccggtcatt ggtcgactcc gtggcccagt tgttcgattg
     2881 cgggcctcta cagtaccgat tcggagtcaa gcaacgatca agtgattggt gccgcggact
     2941 aacgtcgctc acgtagccgg atgggcgtcc gccagggcgc gatacttggc agcgtcggcg
     3001 cgcttgcccc atcgctggta gagggccacc agtagattga caacctggcc ccttcgcttg
     3061 ccgccccaga aatcggtgcg cgccgccaga ttgtggtacg cctccagcag cacagtctct
     3121 gcttcctggt acttgcctag tcgagtcagg caggcgccca gctcccgccg ggccccctgc
     3181 gtgcgattgt cgtccgggcc cgctttggcg agccacccgg tcagcgactc ccggagcagc
     3241 ggctcagcat cacgtgcgct tcccttgtcg gtcttgatcc cacccaggtc catcaggatg
     3301 ccaagcgtga gcggatggcc gggaggcaga accttccgat ccatcgccag cgcctcggtc
     3361 aacgcgcgct ccgcctcggc gagctcaccc ttgtacctga gcaaaaggcc caggttctgc
     3421 aagctctgcg caatgtcctg atccgcgtcg ccgagcagcc tccgccgtct cgccagcgca
     3481 tcgcgcagca cggcctctgc ctcggcgtac tcgccctctt cgcgcagaat cgcgccaagg
     3541 ttgttcagcg cgaagagcgt gttgcggtgg ttctcgccca gggtgcgccg ccaattggcc
     3601 agcacctcgc gcatgttttc ttccgcgccc gcgagattgc ccatgaagta atgaatgacg
     3661 gcgaggctgt tcaccgcgcc gatggtctcg ggatgatccg ggcccagtaa tgaccttcgc
     3721 atcgagagcg tttcgcggag cagcgactcg gcttcgggca tccgccccgc tccctgaagt
     3781 gtcaaggcca gctcgtgcag atcgtcggcc atcaacgggt ggccctgggg atagagccgg
     3841 cgccgctcct gtagcacctc gcgatagagc cgctcggctt ccccgtaatc acccacggct
     3901 tgccggaccg aggcgaggtt gtgcagcgag accagcacgt ccggatggcc ggcgtcgagc
     3961 agctttcgcc gaatcgccag tgcctcgcga ttggcgctgt cggcggcggc gaacttcttc
     4021 gcctctacca gcaccacacc cagctcgttg aggtcctccg ccaccgcgag agatacggcg
     4081 ccggaatgtg cgcggtcgat cgccagcgcc tcccggtgga gggcctcggc ctgagcgtag
     4141 tttgccttcc tccgctgcac cgatcccagc tcccgcaagg tctttgcgac agtcgagtcg
     4201 tcggggccgc gcttgctgcg gcggatggcg agtgcttgcc tgagcaggga gtccgcctcg
     4261 tcatacctcg cctcttcggt gagcacggta gcccagtcag tgagccgctc cgcaacttcg
     4321 ggagccgacc cccccctgag agcccgggtc agtgtgactg cccgaccaag cagactgtcg
     4381 gcccgcggat agagtccgag gtctcgatga atcacgccga ggacgccgag cagctcggcg
     4441 tgcacgtccg ggtggccggc gagagccgtg tccagccgcc gagcgcctcg ctccaggagc
     4501 tcgcgcgcgg tgacgtcacg accgcgcgac tcctgcggac tcgatcctcg gaagagatcg
     4561 accaggactc tcctgacctc gcgctccttg gtcgcttcgt gcgcggcaac tcgcgcctgc
     4621 cacagcgtgc cggc
//
LOCUS       DASRJQ010000017         7425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3087820, whole genome shotgun sequence.
ACCESSION   DASRJQ010000017 DASRJQ010000000
VERSION     DASRJQ010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7425
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3087820"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VFB89_00415"
     CDS             <1..862
                     /locus_tag="VFB89_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HZM25802.1"
                     /translation="EKIVLRILDKGNLTLDLATFGFEPKAQADLLKAILNPYGMVLVT
                     GPTGSGKTTTLYSALSRINTPEVNIMTAEDPVEYNLMGINQVLVRTEIGLTFAAALKA
                     FLRQDPNIIMIGEIRDLETGGIAIKAALTGHLVLSTLHTNDAASTITRMIDMGIEAFN
                     VASAVNLVVAQRLVRRICKDCKGPHQYTDEQLGSLGTDLEKLRPIPFMKGTGCDTCSG
                     TGYKGRAGLYEVMALSPELRRMILRGASVSEMQEQAVTEGMLTLRMDGMKKIERGVTT
                     LEEVVKETAG"
     gene            859..1995
                     /locus_tag="VFB89_00420"
     CDS             859..1995
                     /locus_tag="VFB89_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HZM25803.1"
                     /translation="MSFNLRALLEEMIEKEASDLHITTGERPKLRVDGDIVDSSVTEV
                     LTPKDTLQLAYSVLTENQKKRFETEDELDFSFGIQNLARFRGNCFKQRGCVSMVIRMI
                     PFNVRTFQELGLAPVVAKLAERPRGLILVTGPTGSGKSTTLAAIIDKINKERKGHIIT
                     VEDPIEFIHRHQGCIVNQREVGTDTKNFATALKYSLREDPDVILVGEMRDLETIAAAL
                     TIAETGHLALATLHTNSAAESINRIIDVFPHNQQSQVRAQLAFVLEGVITQTLLPKAK
                     GRGRVMAAEIMVATPAIRALIRDEKIHQIYSAMQAGKKYGMQTMNDALYQLYVAREVT
                     EDECLRVSGDPNEFLRMIGQKPMEDQEISTNGEKPLRAAGATRR"
     gene            2014..3222
                     /locus_tag="VFB89_00425"
     CDS             2014..3222
                     /locus_tag="VFB89_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZM25804.1"
                     /translation="MPMFEYTARNAANGQIQKGQLDVPSQAEVSSYLRKNRLILVSVR
                     EAPKQIKIGLPGAQRIKTRDVVIFTRQFATMINAGLPLVQSLTILAQQTENKALAEIT
                     RAVVYDVESGNTLADAFSKHPRAFSDLYVNMVAAGEAGGILDTILLRLATFLEKSDAL
                     MRKVKGALVYPGVIMTVAGAAIAILLIFVIPVFQSMFSSVNMDLPLPTRIVIMLSDLL
                     IGYWWAILLGIGAVIFAIRRYYGTLSGRKVIDSALLKSPVLGDLLRKSAVSRFTRTLG
                     TLISSGVSILEGLEITAKTAGNMVIHDAVMASRQSIAGGETIAAPLEKSKVFPPMVIS
                     MIAVGEQTGGLDEMLTKIADFYDEEVDVAVGALLSLMEPMMIVVLGVIVGGMVVAMYL
                     PIFDMMNTVQ"
     gene            3450..3941
                     /locus_tag="VFB89_00430"
     CDS             3450..3941
                     /locus_tag="VFB89_00430"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZM25805.1"
                     /translation="MKLVPQLSAVLLDCVEGGASVSFMAPLTREKADAFWRRVAEGVK
                     AGDRLLLVAEDRVTGDIVGTVQLVLGLPENQPHRADVAKMLVRRSARRQGIGAGLMGA
                     VEEAARAASRTLLVLDTVTDSDAERLYVRLGWTRVGVVPNYALWPDGRLCDATFLYKS
                     LSP"
     gene            3938..4807
                     /locus_tag="VFB89_00435"
     CDS             3938..4807
                     /locus_tag="VFB89_00435"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZM25806.1"
                     /translation="MKPFRLPSGTRIGRVVLQIADLERSLAFYQQVIGFRLLTRDDAL
                     SGRRATLGAPDSDHVLLELIEKAGVHPIPYRGRLGIYHFATLLPSRADLGRFLRHVSS
                     LGIRAGSAEHRVSEAMYLVDPDGISMEVYRDRPTDEWPIRDGEVLAPSDPLDVDGLLA
                     AAGSGAWQGVPKGTTVGHMHFYVGDLPAAEAFYHKALGFDRTMWSVPRMLFVAAGGYH
                     HHVGLNTFAADTPVATADDAKLLYWELRLPDAATVSATADNIRRGGYRITEADDHVLA
                     HDPWSIAVRLTHD"
     gene            4816..6399
                     /locus_tag="VFB89_00440"
     CDS             4816..6399
                     /locus_tag="VFB89_00440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HZM25807.1"
                     /translation="MAVFELVIFLLFVGALLSGLARRARLPYPALLALAGAALALIPG
                     TPSVTLDPELALALFVAPVLLDAAFDSSPRDLKENWRAVTGLALAAVGLTVAAVAVVV
                     RWLVPDMPWSAAIALGAIVAPPDAAAATAVLRQLKLPHDVLVILEGESLFNDASALLI
                     YRLAVAATVTGAFSGWGVVPLLLVVCLGSIVLGAVLSRVAMATLPRIHDVATAVIAQF
                     LSTFAVWMIAERLHLSGIITVVVFAITVARSAPDLTPARLRLPSYAVWEVVVFVLNVL
                     AFILVGLQLKPILERLSEREFITYLATGAAVTAAVILVRIVWVMGYAGIGNWVRGRFE
                     GRFTRPARYVSLGAATAIAWCGMRGIVTLAAALALPNGVHGPIAFPYRDLILFSAFAV
                     VLGTLVLQGVTLPPLIRVLRLEDDGSVDREVRLARAETTRAALDAVDRTGAREDMVEL
                     LRRKYQDRLQRAEQVSNGASVTGESAYLIAQRRAQTAQRRKLSELRASGVIGDDAFHR
                     VEEELDWADVNVDGMARGD"
     gene            complement(6396..>7425)
                     /locus_tag="VFB89_00445"
     CDS             complement(6396..>7425)
                     /locus_tag="VFB89_00445"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZM25808.1"
                     /translation="DIKPENILLEAGHAVLADFGIATMVGRPSRVAKTADGTTDPRLT
                     GAGQVVGTPAYMSPEQASGDVIVDGRSDLYSLACVLYEALTGNPPFPGSTAQATIARR
                     FQAPPDSIRAQRPEVPVKLAGTIERALSLDPADRFGSMAEFAAALTAAVQGQQSGGGR
                     LRRGGIALAVGSLALIVVALLAAQRPRHHRHGFDPSRVAVAALSNETGDTTLTSLGDM
                     VASWITDRLGNTSGVSVATSAIVVPAHYDQHVAQPDIDDPQRLERLAQETHAGTLVSG
                     SFYRGSAGAIEFHVEITDANSGRLLRAIGPIISSDPEKTAADLSRIVAATVDTLVKGT
                     GGRRSPRA"
BASE COUNT         1288 a         2357 c         2399 g         1381 t
ORIGIN      
        1 cgaaaagatc gtgctccgaa tcctcgacaa ggggaacctg acgctcgatc tcgcgacctt
       61 cggcttcgag ccgaaggcgc aggcggacct gctcaaggcg atcctgaacc cgtacggcat
      121 ggtgctggtc accggcccga cgggctcggg caagacgacc acgctctact cggcgctttc
      181 gcggatcaac acgccggaag tgaacatcat gaccgccgag gacccggtcg agtacaacct
      241 gatgggcatc aaccaggtgc tcgtccggac cgagatcggc ctcaccttcg cggccgcgct
      301 caaagcgttc ctccggcagg acccgaacat catcatgatc ggcgagatcc gggacctgga
      361 gacgggcggc atcgccatca aggccgcgct taccggacac ctggtgctct ccacgctgca
      421 caccaacgac gcggccagca cgatcacccg tatgatcgac atggggatcg aggccttcaa
      481 cgtggccagc gcggtcaacc tcgtggtggc acagcgattg gtccgccgga tctgcaagga
      541 ctgcaaaggt ccgcaccagt acaccgacga gcaattgggc tccctgggca cagatcttga
      601 gaaactgcgg cccattccgt tcatgaaggg aaccggctgc gacacctgca gcgggaccgg
      661 ctacaagggc cgggcgggcc tctatgaggt catggccctc tcgcccgagc tgcgccggat
      721 gatcctccgg ggcgcctccg tctccgagat gcaggagcag gcggtgaccg aaggcatgct
      781 cacgctgcgc atggatggca tgaagaagat cgagcgcggt gtcacgaccc tcgaagaggt
      841 agtcaaggag acggctggat gagcttcaat ctgcgggcgc tgctcgagga gatgatcgag
      901 aaggaggcgt ccgacctcca tatcaccacc ggcgagcggc ctaagctgcg tgtcgacggc
      961 gacatcgtcg acagctcggt gaccgaggtc ctgacgccca aggacaccct gcagctggcc
     1021 tactcggtcc tgaccgagaa ccagaagaag cggttcgaga ccgaggacga gctcgatttc
     1081 tccttcggaa ttcagaactt agcccgcttc cgcggcaact gtttcaaaca gcgcggctgc
     1141 gtgtccatgg taatccgcat gattcccttc aatgtgcgaa cgttccagga gctgggactg
     1201 gccccagtcg tcgccaagct cgccgagcgg ccccgcgggc tcatcctggt caccggtccc
     1261 acgggctccg gtaagtccac cacgctcgcc gcgatcatcg acaagatcaa caaggagcgg
     1321 aagggtcaca tcatcacggt ggaagacccg atcgagttca ttcaccgcca ccagggctgc
     1381 atcgtcaatc agcgcgaggt cggaaccgat accaagaact tcgcgaccgc actcaagtac
     1441 agcctgcgtg aagaccccga cgtgatcctg gtcggtgaga tgcgcgatct cgagaccatc
     1501 gccgccgcgc tcaccatcgc ggaaaccggc cacctggcgc tcgcgacgtt gcataccaac
     1561 tcggccgccg agtccatcaa ccgcatcatc gacgtctttc cgcacaatca gcagtcccag
     1621 gttcgggcgc agctcgcctt cgtgctcgag ggtgtgatca cccagaccct cctgcccaag
     1681 gccaaggggc gcggccgtgt gatggccgcc gaaatcatgg tggcgacccc ggcgatccgg
     1741 gctctgatcc gcgacgagaa gatccaccag atctattccg cgatgcaggc cggtaagaag
     1801 tacggcatgc agaccatgaa cgacgcgctc taccagctct acgtagcgcg cgaagtgacg
     1861 gaagacgagt gcctccgggt ctcgggcgac ccgaacgagt tcctccggat gatcggccag
     1921 aagcccatgg aggatcagga gatctcaacc aacggcgaga agccactgcg cgcagccggc
     1981 gccacccgtc gatagcggaa gagggatcca tccatgccga tgttcgagta caccgcgcgt
     2041 aacgcggcca acgggcagat ccagaagggc cagctcgacg tcccgagcca ggccgaggtc
     2101 tccagttatc tccggaagaa ccggctgatc ctggtgagcg tccgcgaggc gcccaagcag
     2161 atcaagatcg gcctgccggg ggcgcagcgg atcaagaccc gtgacgtcgt gatcttcaca
     2221 cggcagttcg cgaccatgat caacgcgggc ttgcccctgg tgcagtcgtt gacgatcctg
     2281 gcccagcaga ccgagaacaa ggcgcttgcc gagatcaccc gcgcggtggt gtacgacgtc
     2341 gagtccggca acaccctggc cgacgcgttc agcaagcatc cgcgcgcgtt cagcgatctc
     2401 tacgtcaaca tggtggcggc cggcgaggcc ggcggtattc tcgatacgat tctgctccgg
     2461 cttgcgacct tcctggaaaa gagcgacgcc ctcatgcgga aggtgaaggg cgcgctggtg
     2521 taccccggcg tgatcatgac ggtggccgga gccgcgatcg cgatcctcct gatcttcgtg
     2581 atcccggtct tccagagcat gttctcgtcg gtcaacatgg atctgccgct gccgacgcgg
     2641 atcgtgatca tgctctcgga tcttctgatc ggctactggt gggccatcct gctcggcatc
     2701 ggcgcagtga tctttgccat ccggcggtac tacgggaccc tgagcgggcg gaaggtcatc
     2761 gactccgcgc tgctcaagtc cccggtgctg ggcgacctgc tccggaagtc ggccgtttcg
     2821 cggtttaccc ggacgctcgg caccctgatc tcatccggtg tatcgattct cgaaggcctc
     2881 gagatcaccg ccaagacggc cggcaacatg gtcatccacg atgccgtcat ggcctcccgg
     2941 cagtcgatcg ccggtggtga gacgatcgcc gctccgctgg agaagtcgaa ggtgttcccg
     3001 ccgatggtga tctcgatgat cgcggtgggt gagcagaccg gtggtctgga cgagatgttg
     3061 accaagatcg cggacttcta cgacgaggaa gtggacgtgg cggtgggcgc gctgctctcg
     3121 ctcatggaac cgatgatgat cgtggtcctg ggcgtgatcg tcggtggcat ggtggtagcg
     3181 atgtacctgc cgatcttcga catgatgaac acggtgcagt aagacccgaa cgcagcctca
     3241 taactcggaa gcggcgcccg tgcaagcaac gggcgccgct tcgctttctg acaggattgt
     3301 tcgatcgctg ggttctatct tgcgggcgtg gctaccggcg cgaatagctt accaggaatc
     3361 cgacggctga acccctggtc gaggtgcctt tgcctgcggt gaaatcgcac gaccccgcaa
     3421 cggtccgctg ccttactgcg gacgaagccg tgaaactcgt tccgcagctt tcggcggtgc
     3481 tgctcgactg cgtcgagggt ggcgcgtccg tcagcttcat ggcgcccctg acccgggaga
     3541 aggcggacgc cttctggcga cgcgttgccg agggcgtgaa agccggcgac aggctattgc
     3601 tcgtggccga ggaccgcgtt accggcgaca tcgtcgggac agtgcagctc gtcctcgggc
     3661 tccccgagaa tcagccgcac cgagccgacg tggcaaagat gctggtccgt cggtcggccc
     3721 ggcggcaggg gatcggggcg ggtctgatgg gggccgtcga ggaggctgcg cgagcggcaa
     3781 gccggaccct gctcgtgctc gacacagtca ccgacagtga tgctgaacgg ctgtatgttc
     3841 ggcttggatg gaccagggtg ggcgtcgtgc cgaattacgc cctctggccg gatgggcggc
     3901 tatgcgacgc gacctttctc tacaagtctc tgagcccttg aaaccctttc gtcttccgtc
     3961 cggtacccgc atcggccgcg tggtgcttca gattgccgac ctcgagcggt cgcttgcctt
     4021 ttatcagcaa gtcatcgggt ttcggctgct cacccgggac gatgctctca gcggtagacg
     4081 tgcgacgctt ggtgcgcctg acagcgatca cgtcctcctc gagctgattg agaaagccgg
     4141 agtgcatccc attccatacc ggggtcgcct cggcatctac cactttgcca ctctgcttcc
     4201 gagtcgggcc gaccttggcc gttttctgcg acacgtctca tcactcggga ttcgagccgg
     4261 ctccgcagag caccgcgtca gtgaggcgat gtacctggta gatccggatg gcatctcgat
     4321 ggaggtgtat cgggatcggc cgaccgacga gtggccgatc cgcgatggtg aggtgctggc
     4381 tccgagcgat ccgctggacg tggatggact gctcgcggct gccggatccg gagcatggca
     4441 gggtgtgccg aaaggcacaa ccgtcggtca catgcatttc tacgtcggcg atcttcctgc
     4501 ggctgaggcc ttctaccaca aagcgctggg cttcgaccgg acgatgtgga gcgtaccgcg
     4561 catgctattc gtcgccgccg gcggatacca ccatcatgtc ggactcaaca cgtttgcagc
     4621 cgacactccg gtcgccaccg ctgacgatgc gaagctcttg tactgggagc ttcgcctccc
     4681 ggatgcagcc acggtgagcg caacggccga caacattcgc cgcggagggt atcgaattac
     4741 cgaggcggat gatcacgtcc tggcccacga tccctggtcc attgcggtgc ggcttacgca
     4801 cgactgagcg acaacgtggc cgtattcgag ctggtcatct tcctgctgtt tgtgggggct
     4861 ctgctctccg gcctggcccg tcgcgctcgg cttccttacc cggcattgct ggcgcttgca
     4921 ggcgccgccc tagctctgat ccccggtacg cccagcgtga ccctcgatcc cgagcttgcg
     4981 ctggcgctct tcgtcgcgcc ggttctactc gatgctgcgt tcgactcgtc gccccgcgac
     5041 ctcaaggaaa actggcgtgc ggtgaccggc ctcgcgctgg ccgccgtggg gctaaccgtc
     5101 gccgcggtgg ctgtggttgt ccgctggctg gtaccggata tgccttggtc ggctgcgatc
     5161 gcgctgggcg caatcgtggc gccgcccgac gctgctgccg cgaccgcagt gcttcgacag
     5221 ctcaagctgc cgcacgatgt tctggtaatc ctcgaggggg aaagcctttt caacgacgcg
     5281 agcgctctgc tcatctatcg ccttgcggtc gccgcaaccg tgactggagc attctcgggc
     5341 tggggtgtag tcccgttgct gctcgtggtg tgtctcggca gcatcgttct cggtgccgtt
     5401 ctgtcgcggg ttgcgatggc gacccttccc cggattcacg acgtcgcgac ggccgtcatc
     5461 gcccagttcc tttcgacttt cgcggtttgg atgatcgccg agcgactgca cctctccggc
     5521 atcatcaccg tcgtggtatt tgcgatcacg gttgcccgga gcgcgccgga cctgacgccg
     5581 gcgcggctgc gacttccctc gtacgcggtg tgggaagtgg tagtgttcgt cctcaacgtg
     5641 ctggctttca tcctcgtcgg tctccagctc aagccgatcc tcgagcggct gagcgaaaga
     5701 gagttcatca cctatctggc gacgggtgca gcggtcacgg ctgcagtgat cctggtgcga
     5761 atcgtctggg tcatggggta tgccggcatt ggtaactggg tcaggggtcg gtttgaggga
     5821 cgattcaccc ggccggcccg ctacgtctcg ctcggcgctg caacggcgat tgcgtggtgc
     5881 ggcatgcggg gcatcgtgac actcgctgcg gcactcgcac ttcccaatgg agtgcacgga
     5941 cccatcgcgt ttccctaccg ggatctcatt ctgttttccg ctttcgctgt cgtgcttggc
     6001 acactggtgc ttcagggtgt gactctgccg cctctgatcc gggtcctgcg attggaggac
     6061 gacggatcgg tggaccgcga ggtgcgccta gcacgggccg aaacgacccg agcggcgctc
     6121 gacgctgtag accgaactgg agcgcgcgag gatatggtcg agctgctccg ccgcaagtat
     6181 caggatcggt tgcagcgggc tgagcaggtc agcaacggcg cgtccgtcac cggcgaaagc
     6241 gcctatctga tcgcccaacg gcgcgcgcag acggcccagc ggcgaaagct ctcggagcta
     6301 cgtgcgagcg gcgttatcgg agacgatgcc tttcaccgcg tcgaagaaga gctggactgg
     6361 gcggacgtga acgtcgacgg aatggctcgg ggcgattagg cccgagggct gcgccgaccg
     6421 cctgtgccct tgaccagcgt atcgaccgtg gccgccacga tgcggctcag gtccgctgcg
     6481 gtcttctctg gatcggagct tatgatcggt ccgattgcac gtaggagtcg gccgctgttt
     6541 gcatcggtga tctctacgtg gaactcgatg gcccctgccg agccccggta gaacgagccc
     6601 gagaccagcg tccctgcatg cgtttcctgg gccaggcgct ctaaccgctg agggtcgtcg
     6661 atgtcaggct gcgcgacgtg ctggtcgtaa tgtgccggca ccacaatggc tgacgtggcg
     6721 acggacacac cggaggtatt tcccaaccga tccgtgatcc aggacgcgac catgtcgccg
     6781 agcgacgtca aggtcgtgtc gccagtttcg ttactgaggg cagcgaccgc gactcggctc
     6841 ggatcgaagc cgtggcgatg gtggcggggt ctctgtgccg ccaggagtgc taccacgatg
     6901 agcgcgagcg atccgacggc caaggcgatg ccgccgcgac gcagccgtcc cccgccagac
     6961 tgttgtccct gtaccgcagc ggtcaatgca gcggcgaact cggccatgct gccgaagcga
     7021 tcggctgggt cgagtgaaag cgctcgctcg attgtgccgg cgagcttgac tgggacctcg
     7081 ggccgttggg cccgaattga gtccggggga gcctgaaagc ggcgagctat tgtcgcctga
     7141 gcggtgctgc cgggaaaggg tggattgccg gtcaacgcct cgtacagcac gcatgccagg
     7201 gaatagagat cactccgtcc gtccacaatc acatcgccac tggcctgctc cgggctcatg
     7261 taggccgggg ttcccacaac ctgcccggct ccggtcaggc gagggtctgt cgtcccatcg
     7321 gcggtcttgg ccactcggct ggggcgaccc accatagtcg cgatgccaaa gtccgccagc
     7381 acggcgtggc cggcctcgag gagtatgttc tccggcttga tatca
//
LOCUS       DASRJQ010000018         2477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_830600, whole genome shotgun sequence.
ACCESSION   DASRJQ010000018 DASRJQ010000000
VERSION     DASRJQ010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830600"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="VFB89_00450"
     CDS             <1..883
                     /locus_tag="VFB89_00450"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HZM25809.1"
                     /translation="DLMRLEIEQPTHLVDISRLPLKGIEELGDGGIRIGAQALNSDTA
                     AHPRVRTRYPLVSQALVAGASGQLRNKASIGGNLLQRTRCPYFYDTDAACNKRKPGSG
                     CAAIDGINRIHAILGASDACIAVHPSDMAVALAALDARVELLRPGGSTRQVGIGEFYR
                     LPGDTPHVETVVQPGEMITAVELPAPPAGRQLYRKVRDRASYEFALVSVAVVVAVEQG
                     RIKVARVAFGGVSPQPWRSAGAETALVGRPATIATYQAAAQAAMKNAVGRGHNDFKIE
                     LAKRTLVTTLARAAQEG"
     gene            886..>2477
                     /locus_tag="VFB89_00455"
     CDS             886..>2477
                     /locus_tag="VFB89_00455"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HZM25810.1"
                     /translation="MATLTGQPINRVDGPLKVSGRATYAYEHWDVGQPLYGFIVGATI
                     GKGRIKRIDASRAERAPGVQLILTHQNAPEQGTPDLSVPSQYSRAFPTLSGPEVHHYG
                     EAVALVVATTLEQARAAGSLIDVEYSVEPGRFDFAAELDKAFAPKAVNAGLPTDSVVG
                     DFDAAFAKAEVKVDQIYTTPYEFSHPMELNACLAVAKGEDLYLYVSAQMLGEAREAVA
                     STFKIDPKRIHLITPFVGGGFGSKLGIHSETILAAMAARALQQPVKVSITRQQIFSLV
                     SARPITTQRVRFGAGRDGKLVAIGHDVTMHINPTAEYAEQSATPARELYAAPNRLTRH
                     RLVPLDMVRGEDVRAPGEAPGLLAFESGMDELAHALDMDPIELRILNEPKTHPERGIP
                     FSDRRMVECMREGARRFGWERRPKKPGTLRDGRWLVGYGMAGSIRMQFQAATKVSVRM
                     GPDGVVVVQSDMTDLGTGTYTIVTQVVADALDLPLDHVRVELGRSEYPAGAGSGGSWG
                     ATNTCTAATRACKALKEKLKGG"
BASE COUNT          439 a          808 c          846 g          384 t
ORIGIN      
        1 cgatctgatg cgactggaga tcgagcagcc aacccatctc gtcgacatca gccggctgcc
       61 gctcaagggg atcgaagagc tgggcgacgg cgggatcaga atcggggccc aggcgctcaa
      121 ctccgacacc gccgctcacc cgcgggtgcg cacccgctat cccctggtct cccaggcgct
      181 ggtggccggc gcttccggcc agttgcgcaa caaggcatcc atcggcggca atctgctcca
      241 acgcacccgc tgcccgtact tctacgacac cgacgccgcc tgcaacaagc gcaagcccgg
      301 ctccggctgc gccgcgatcg acggcatcaa tcggatccac gcgattctgg gcgcgagcga
      361 cgcgtgcatc gccgtccatc cgtccgacat ggccgtcgcc ctcgccgctc tcgatgcacg
      421 ggtcgagctg ctgcgacccg gcggctcgac tcgccaggtc ggtattggcg agttctaccg
      481 cctccccggc gacacaccgc acgtcgagac cgtcgtccag ccgggcgaga tgatcaccgc
      541 ggtcgagctg cccgccccgc cggcgggtcg ccagctctac cgcaaggtgc gcgaccgggc
      601 ctcctacgaa ttcgccctgg tttccgtcgc agtcgtcgtt gcggtggagc agggaaggat
      661 caaggtggca cgggttgcat tcggcggggt gtcaccccag ccgtggcgct cggccggggc
      721 tgagacagcg ctcgtaggcc gcccggcgac gatcgcgaca tatcaggctg ccgcgcaggc
      781 ggccatgaag aacgccgtgg gacggggaca caacgacttc aagatcgagc tggccaagcg
      841 cacgctggtg accacgcttg cccgcgccgc acaggagggc tgaccatggc caccctcacg
      901 ggacaaccca tcaaccgggt ggatggaccg ctcaaggtga gcggcagggc cacctacgca
      961 tacgagcact gggacgtggg tcagccgctg tatggcttca ttgtgggagc cacgatcggc
     1021 aagggcagga tcaagcggat cgacgcatcc cgtgcggagc gcgctcccgg cgtgcaactg
     1081 atcctcactc accagaacgc gccggagcag ggaacgcccg acctgtcggt tccgtcgcag
     1141 tactctcgtg catttccgac actgagcggg cccgaggttc accattacgg cgaagcggtt
     1201 gccctggtgg tcgccactac gctggagcag gctcgcgcgg ccgggagtct aatcgacgtg
     1261 gaatattcgg tcgagccggg acggttcgac tttgccgcgg agctggacaa ggcgttcgca
     1321 ccgaaagccg tgaatgcggg actgcccact gacagtgtgg tcggcgattt cgacgcggcg
     1381 ttcgccaagg cggaggtgaa ggtcgatcag atctacacga cgccgtacga gttctcccat
     1441 ccgatggagc tgaacgcctg tctcgcagtc gcaaaaggcg aggacctgta cctctatgtc
     1501 agcgcccaga tgctgggcga ggcccgcgag gcggtcgcca gcacgttcaa gattgatccg
     1561 aagcgaattc acctcatcac tccgtttgtc ggcggggggt tcggctcaaa gctgggcatt
     1621 cactccgaga ccattctggc ggcgatggcg gcccgcgccc tccagcagcc ggtcaaggtc
     1681 agcatcactc gacagcagat cttcagcctg gtgagcgcgc gccccataac cacccagcga
     1741 gtgcggtttg gggccgggcg ggacgggaag ctggtggcga tcggtcacga tgtaaccatg
     1801 cacatcaacc cgaccgcgga gtatgcagag cagagcgcca caccggcgcg agagctctac
     1861 gccgctccca atcggctgac ccgtcaccgg ctggtgccgc tggacatggt gcggggggag
     1921 gacgttcgag cgccgggtga agcgccgggt ctccttgcgt tcgagtccgg catggacgag
     1981 ctggcgcacg ccctcgacat ggacccgatc gagttgcgta tcctgaacga gcccaagacc
     2041 catcccgagc ggggcatccc gttcagtgat cggcgcatgg tcgagtgcat gcgcgaaggc
     2101 gcgcggaggt tcggatggga gcgccggcca aagaagccgg gcacgctgcg cgacggccgg
     2161 tggctggtgg gttacgggat ggcgggctcc atccggatgc agttccaggc cgcgaccaag
     2221 gtgagtgtga ggatgggtcc cgacggagtg gtcgtggtgc aatcggacat gaccgatctt
     2281 ggcaccggca cctacacgat cgtgactcag gttgtggcgg acgcgctgga tctgccgctc
     2341 gatcacgtgc gagtcgagct gggacggtcg gagtatccgg ccggcgcggg ctcgggaggc
     2401 tcctggggcg cgaccaatac ctgcacggcg gctacccgtg cctgcaaggc gctcaaggag
     2461 aagctcaagg gcggcaa
//
LOCUS       DASRJQ010000019         2450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1656097, whole genome shotgun sequence.
ACCESSION   DASRJQ010000019 DASRJQ010000000
VERSION     DASRJQ010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656097"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VFB89_00460"
     CDS             <1..244
                     /locus_tag="VFB89_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25811.1"
                     /translation="QLAGEAISKSGEELAKFIEDAGEEVTRLARQYSTESKELAVKLR
                     EMARDETDRLNHFLTKIQELRDAMTEAQVKFLGAGQ"
     gene            complement(489..1124)
                     /locus_tag="VFB89_00465"
     CDS             complement(489..1124)
                     /locus_tag="VFB89_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25812.1"
                     /translation="MAVLCMIIPLSEMGNVPAHPIAPGGPPPGIWPSPGHPAHPIAPG
                     GQPPGIWGGAPSYPDQGLPGQPPGIWPSPGHPAHPIAPGGPPPGIWPSPGHPAHPIAP
                     GGRPPGIWGGAPSYPDQGLPGPQPIPTPPIYLPPGTIPGHPEHPIYIPPSIWPSPGVP
                     THPIVLPPAGSGEKPEVLENWEVKTAWSATTGWVVAIVPTEEHPGTPTPSR"
     gene            complement(1207..1380)
                     /locus_tag="VFB89_00470"
     CDS             complement(1207..1380)
                     /locus_tag="VFB89_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25813.1"
                     /translation="MTLGLCYWILMLIWFVFGLLVHFNVVGGPYGLVGSSLLLFVLFL
                     LLGWQVFGPPLHR"
     gene            complement(1476..>2450)
                     /locus_tag="VFB89_00475"
     CDS             complement(1476..>2450)
                     /locus_tag="VFB89_00475"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HZM25814.1"
                     /translation="EDRPTLERGDEGPDVVDLQSMLPKFTGDVDGEFGPETEENVIRY
                     QTSRGLEVDGIVGEETWQALYNNEPPLPPPEPPPGALTYNQQAAIIKIARDSDIADYS
                     WDDRGKAPAGYTQGMALAFAQTYLKLKADHPAAVEMSKARTSSDKDALNEYLDEFRSH
                     GMSNEHDGVDTLRHLYALMLGSGMRESSGRHCEGRDMSAENMSSDTCEAGLFQTSYNA
                     HGASDPEFDQLMHEYSLPKNEPTCYLEAFREGVSCSSSEWDSYGSGAGREFQDLCKNC
                     PAFAVETHGLTLRNLCNHYGPIIRHEVELKREADEMFKEVQDYIDSVA"
BASE COUNT          451 a          771 c          799 g          429 t
ORIGIN      
        1 ccagctcgcc ggcgaggcga tcagcaagtc cggcgaggag ctcgccaagt tcatcgagga
       61 cgccggagaa gaggtcaccc ggctcgcgcg gcagtacagc accgagagca aggagctcgc
      121 cgtgaagctc cgggagatgg cccgagacga gactgaccgg ctcaaccact tcctgaccaa
      181 gatccaggaa ctccgggacg ccatgaccga ggctcaagtg aagttcctag gggcggggca
      241 gtgacaagaa gaaagacccg gcgggagggg gacctcccga ccgggcctgt caactgggag
      301 aacgaggggt cgtccctcga cggccgccct aagtgcctca cccgccgggg tgaggacttg
      361 ctcttcgaga gactaagaga ggcccacccg ctgggcgcgt ccgggggcga gccacgccct
      421 ccggatgacg aggggtaggg taaccaaccc cacccccacg tttcggcccc agcgggcccc
      481 tattttgatc accgtgacgg ggtcggtgtg ccggggtgct cctccgtcgg cacgatcgcg
      541 acgacccaac cagtcgtcgc tgaccaggcg gtcttcacct cccagttctc cagaacctcc
      601 ggtttctcac cactgccggc cggcggcaac acgatcgggt gcgtcggcac gccggggctc
      661 ggccaaatgc tcggcgggat gtagatcggg tgctcgggat gtcctggaat tgttccaggc
      721 ggcaggtaga tcggcggcgt cgggatcggt tgcggaccgg gaagtccttg gtcgggatag
      781 gacggcgcac ctccccagat gcctggaggg cgtccgccgg gtgcgatggg gtgcgcggga
      841 tggccgggtg acggccagat gcccggaggc gggccgcccg gtgcgatggg atgcgctgga
      901 tgtcccggcg acggccagat accaggtggc tgtccaggga gcccttgatc aggataggac
      961 ggcgctccac cccagatgcc aggaggctga ccaccgggcg cgatcggatg cgctgggtga
     1021 ccagggctcg gccagatgcc tggcggagga ccaccagggg cgattgggtg agctggcacg
     1081 ttgcccatct cactgagcgg gatgatcatg cagaggactg ccatcgcgaa actcctttgt
     1141 tttcactgag agaggaacaa ggggagtaag gcctctctac tctaactcac gagaggttcg
     1201 taaacattat ctgtgcagcg gaggtccgaa gacctgccat cctagcagta agaacagcac
     1261 gaacagcagc aagctgctac cgacgaggcc gtatggtcca cccaccacgt tgaagtggac
     1321 gagcagaccg aacacgaacc agatcagcat caagatccag tagcagagac cgagcgtcat
     1381 gaccgtctcc tcctatcgca tcactatcca ccacgctgct gcgcccacca tggccgctac
     1441 cagcaccgcg cagatgtaga tcgctacgtc ttctttcacg cgacgctgtc aatgtagtct
     1501 tggacttcct tgaacatctc gtcggcttcg cgcttgagct cgacctcgtg gcggatgatc
     1561 gggccgtagt ggttgcacaa gttgcgcaag gtcaggccgt gggtctccac agcgaacgca
     1621 gggcaattct tgcacaagtc ctggaactcg cggcccgcac ctgagccgta cgagtcccac
     1681 tcgctcgagc tgcacgacac cccctcgcgg aaagcttcga ggtagcacgt cggttcgttc
     1741 ttcggcagcg agtactcgtg catgagctgg tcgaactcag ggtcactggc gccgtgggcg
     1801 ttgtacgaag tctggaacag cccagcctcg cacgtgtcgc tcgacatgtt ctccgcgctc
     1861 atgtcgcggc cctcgcagtg gcggcctgac gactcccgca tgccactgcc gagcatcaag
     1921 gcgtagaggt gccgcagcgt gtcgacgccg tcatgctcgt tgctcatgcc atgcgagcgg
     1981 aactcatcga gatactcgtt gagcgcgtcc ttgtccgagc tcgtgcgcgc cttggacatc
     2041 tccaccgcag ctgggtggtc agccttgagt ttgaggtacg tctgcgcgaa cgctagagcc
     2101 atgccctggg tgtaaccggc gggcgccttg ccacggtcat cccaggagta gtccgcgatg
     2161 tcagagtcac gagcgatctt gatgatcgct gcctgctgat tgtaggtcaa cgcgccaggg
     2221 ggaggctcag gtggtggcag cggtggctcg ttgttgtaaa gagcctgcca cgtctcctcg
     2281 ccgacgatgc cgtcgacctc tagtccgcgc gacgtctggt agcggatcac gttctcctcg
     2341 gtctccggtc cgaactcgcc atcgacgtcg ccggtgaact tcggaagcat cgactggaga
     2401 tcgacgacgt ccggtccctc gtcgccgcgc tccagcgtcg gccggtcctc
//
LOCUS       DASRJQ010000020         2928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3934157, whole genome shotgun sequence.
ACCESSION   DASRJQ010000020 DASRJQ010000000
VERSION     DASRJQ010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3934157"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="VFB89_00480"
     CDS             <1..904
                     /locus_tag="VFB89_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25815.1"
                     /translation="EARELALRSTSRLSDEQAGPLVSVLRRRPIAIAQACGYLERTPT
                     TSPDVLQGLLERRRALTLETAAETTERNLKLLHAATLRKLDTTYPAAARLLEFLAFWP
                     VQGLQGRLDDSAGAYEDQGNEKRNDDGYAVAVCYLMLALGLPLDDLEVGLMVYSRSIE
                     LLHERYLIKGGYRGAWMHGVTAAVFRSLFEASAQRIEGRIADVYEASTRAWALDLLED
                     DESARQNYLRFLEVVFPPIHRDLTAALGDIESHNDELVRVLQALFLRESGGVRQKLAD
                     VWKQPHEVVDPNDPAWRQHLAPTD"
     gene            complement(917..1714)
                     /locus_tag="VFB89_00485"
     CDS             complement(917..1714)
                     /locus_tag="VFB89_00485"
                     /inference="COORDINATES: protein motif:HMM:NF013003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HZM25816.1"
                     /translation="MVDTEATRPARQSAGYWPTALTAIAAAVTVAVLALTAAYTERSN
                     RNQLDLAEQGQITDRFSKAIDQLGREDAKDLSLRLGGIYALERIMRDSDDDEAAIVEV
                     LSAFVREHTRIGAKPIRNPTTDVQAALTVLGRRPNADQRGFGTDAGFGIIDLRDAPPQ
                     EVHLEKASLQGAHLEGALLKGAHLDAANLRVAHLEGADLEGTVLNGADLEGAHFEKAR
                     LKNTYVGGTDLSKTTGLTTESVREVCADKDTTFPPGVTGPTGTIEGC"
     gene            complement(1811..2011)
                     /locus_tag="VFB89_00490"
     CDS             complement(1811..2011)
                     /locus_tag="VFB89_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25817.1"
                     /translation="MRVETIRFVARNEDEAYFIRAMHQLAAGCLLLGAEGGGVDGDGG
                     SGEWGGWGTTGATGAGAVPVGV"
     gene            complement(2012..>2928)
                     /locus_tag="VFB89_00495"
     CDS             complement(2012..>2928)
                     /locus_tag="VFB89_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25818.1"
                     /translation="LGVGFVAGFVTFRRSLRWCVTCGAVLRCTECTQRRPRPEAAPVK
                     PPQRPPLVPGDVLRVEEPDYLYGVGPAVLRVTAVGVAERLPDGWWLNLRGIAVRPDGS
                     PRCTGSTYQRMIVTAERNEASAERQQPDGEPGRNDLLRQARLRTCSPDDPTRAMSQRE
                     LAEAVTAYVQRTTGRDVALDRHDISRWERGGRRWPDAHYRDAFRAVLGVATDAELGFY
                     IKSRPSVGARPARSVTPSSTAITANGGRAQPSGADVMAAQLVVNPGPAVILASATPEL
                     LNAVVPLLAALKRYHVGDEPSLTTVGTG"
BASE COUNT          520 a          943 c          908 g          557 t
ORIGIN      
        1 tgaggcccgc gaactagcac tgcgcagcac ctcccgactc tcagacgagc aggctggccc
       61 actggtatcg gtgctgcggc gccggccaat cgccatagcg caggcttgtg gctaccttga
      121 gcggacccca acaacgtcgc cggacgtcct gcaaggactc cttgagcgtc gaagggcgct
      181 gaccctcgaa acggctgctg agaccactga aagaaatctg aagctgcttc atgccgccac
      241 gcttcgaaag ctggatacca cctacccggc ggcagcccgg ttgcttgagt tcttggcctt
      301 ttggcccgtg caggggctac agggccgatt ggacgatagc gcgggagcct acgaagacca
      361 gggcaacgag aaacgaaatg atgatggtta cgcggtagcg gtctgctatc tgatgctggc
      421 gctcggccta ccgctcgacg acctagaagt cggcctgatg gtctactcca gatcaataga
      481 actgctccac gagcgctatc tcattaaggg cgggtatcga ggtgcctgga tgcacggagt
      541 tacggcggca gtcttccgca gtttgttcga ggcgtccgcc cagcgcatag agggacgaat
      601 agcggatgtc tacgaggcca gtacgcgcgc ctgggcacta gacctgctgg aagatgacga
      661 atctgccagg cagaactacc tgcgtttcct tgaggtggta ttccccccta tccatcggga
      721 tctgacggca gcgctaggag atatagagag ccacaatgac gagctggtgc gcgtactaca
      781 ggcgctcttc ttgcgggaat cgggtggcgt caggcagaag ctcgctgatg tttggaagca
      841 accacacgaa gtagtagacc ctaacgaccc cgcatggagg cagcatctcg cgcctacaga
      901 ttgactgagc gacctctcag cacccctcga tcgtgccggt ggggccagtt acgccaggcg
      961 gaaacgttgt gtccttgtcg gcgcagactt cccgcacact ctcggttgtc agacctgtgg
     1021 ttttggaaag gtccgtgccg ccgacgtatg tgtttttcag gcgtgccttt tcaaagtgcg
     1081 cgccctccag gtcggccccg tttagcacgg taccttccag gtccgcgcct tccagatggg
     1141 cgacgcgtag gttcgccgcg tctaggtggg ctccttttag gagcgcaccc tccaaatgtg
     1201 caccctgcaa gctcgccttc tccagatgca cctcctgcgg aggggcgtcc ctaaggtcga
     1261 tgatgccgaa tcctgcatcg gtgccgaatc cccgctggtc ggcgttgggt cgccgtccaa
     1321 gcaccgtcag cgcagcctgt acatccgtcg tcggatttcg gatgggcttt gcaccaatac
     1381 gtgtgtgctc gcggacaaat gcgctaagca cttcaacgat cgcggcttcg tcgtcgtcgg
     1441 agtcccgcat gatccgttca agggcgtaaa ttccgcccag tcgcagactg agatccttgg
     1501 cgtcttcgcg gcccagttga tcgatcgcct tgctgaaccg gtcagtgatc tgaccttgtt
     1561 cagcgagatc tagctgatta cggttcgacc gctccgtata cgcggcagta agagccagca
     1621 ccgcgacagt gactgcggca gcgatggccg ttagagcggt cggccaataa cccgcggatt
     1681 gcctcgccgg acgcgtcgct tcggtgtcca ccatgctgcg gacggtaact acattcgtag
     1741 tcaattccga tcgtccgtgt ggatcgaaga tagaaatgat cggcgttgcg cctggcgctt
     1801 ggttcagatt tcagacgcct accggcacgg cacccgctcc tgttgcccct gtcgttcccc
     1861 aaccacccca ctcaccactc ccaccatcgc cgtcaacgcc tcctccctcg gcccccagga
     1921 gcaaacatcc tgccgctaac tggtgcatcg cccggatgaa gtacgcctcg tcctcgtttc
     1981 gggcgacgaa gcgaatcgtc tcaaccctca tctaccccgt ccccacggtt gtcagggatg
     2041 gctcatcgcc tacgtggtag cgcttcagag cggccagtag gggaacgacc gcgttcaaca
     2101 gttccggtgt ggcggacgcc aggatcacgg cagggccagg gttgaccacg agctgtgcgg
     2161 ccatgacgtc agcgccgctg ggttgggctc tacccccgtt cgccgtgatc gccgtcgagg
     2221 aaggggtgac tgatcgggca gggcgggctc ctaccgacgg acggctcttg atgtagaagc
     2281 ccagctcggc gtcagtggca acacccagca ccgcccggaa cgcgtcgcgg tagtgggcgt
     2341 cgggccatcg ccggccacca cgttcccacc ggctgatgtc gtgccgatcc aaggccacgt
     2401 ctcttccggt ggtgcgctgg acgtatgcgg taaccgcctc ggccagctcg cgttgggaca
     2461 tcgctcgggt ggggtcatcg ggggagcagg ttcgtaaccg tgcctgccgc agcaggtcgt
     2521 tgcggccggg ttcaccgtct ggctgctgcc gctcggccga tgcctcgttt cgttcagcgg
     2581 tcacaatcat cctttggtat gtgctgccgg tgcagcgcgg cgagccgtcc ggccgtaccg
     2641 cgatcccgcg caggttgagc caccagccat cgggcaaccg ttcggccaca cccacggcgg
     2701 tgacccgcag cacggcgggg ccgacgccgt agaggtagtc cggctcctcg acgcggagca
     2761 cgtcaccggg caccagtggc ggacgctgcg gcggcttcac gggcgcggct tccggtcgag
     2821 gtcggcgttg ggtgcactcc gtacagcgca gcacggcccc gcaggtcacg caccaccgca
     2881 gcgaacggcg gaacgtcacg aacccggcta cgaagccgac gcccagcg
//
LOCUS       DASRJQ010000021         3714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_4203623, whole genome shotgun sequence.
ACCESSION   DASRJQ010000021 DASRJQ010000000
VERSION     DASRJQ010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4203623"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VFB89_00500"
     CDS             complement(<1..207)
                     /locus_tag="VFB89_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25819.1"
                     /translation="MSEPVAAQDTAGVIAPPPLILLGGLVLAITAERLYPLPWVPLVL
                     RAPGGALLVLAAAVLLVLSFREFRA"
     gene            378..1259
                     /locus_tag="VFB89_00505"
     CDS             378..1259
                     /locus_tag="VFB89_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZM25820.1"
                     /translation="MFRTFRAALTAATVALAAVTAVEAQAAGQRVKVNGMQMYYEVSG
                     NGTPLIVLHGAYMNIPMMGTIIPTLAKTHKVYALELQGHGRTTDIDRPITYPNLADDV
                     AAFMDAVGLQSADVFGYSMGAAVGLQLAIRHPGKVNKLAAASVAYDVEGWQPEFKAFI
                     PQMKVEMFLEMPFAKDYRKLAANPDGFPELVKKLIALEKEPMAWAEQVKALKAPVLII
                     TGDADVATLEHSVSLFRMLGGGGMGDMGKPLPASRLAVIPATAHTAVITQPDLLYAFI
                     EPFLKGQTPKGMFEGAK"
     gene            complement(1201..2154)
                     /locus_tag="VFB89_00510"
     CDS             complement(1201..2154)
                     /locus_tag="VFB89_00510"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZM25821.1"
                     /translation="MGTAWKKGLAYAVIYLVWGSTFLAIRICVQEVPPFLCASIRFLL
                     AGALLWGWTLARRERQPRGRERLTTVVVAFLIFVLDYGLVFWAERKVPSGIAAVMLAT
                     IPLFMAISEILVLRERRLTLRLAIALLMGITGVAVLTSRTLGLGGTPVPRAEAVGLII
                     GAMSWAVASVLTRRLPLPASKVVTSGSQMFVGGVLLSLIAAGLGEARAFDPSTVSPKA
                     WLALAYLIVPGSIVAFTAYTWLIQVDSPTRVGTYAYVNPIVAVLLGYFAGGEDLSLRT
                     VLGSLLVLVSVVVIMAESRGKGLTSRPRTSPSASAPSGTAR"
     gene            complement(2233..2643)
                     /locus_tag="VFB89_00515"
     CDS             complement(2233..2643)
                     /locus_tag="VFB89_00515"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance family protein"
                     /protein_id="HZM25822.1"
                     /translation="MLVHRLNPILNVSDIQQSFEWFEKLGWRKSWDWGSPPTFGGVCS
                     GVCEIFLCLDGQGGRGASGSPMTFGPESNQSAERSVWMSIWVDDVDEIHRRCLEQGLE
                     VTWPPTDMPWNVREMHVRHPDGHVFRISGERDNG"
     gene            2989..3282
                     /locus_tag="VFB89_00520"
     CDS             2989..3282
                     /locus_tag="VFB89_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25823.1"
                     /translation="MIIVREIFIAKPGMASKLATKFKEEMAGESSGNGFKVLTDVVGS
                     YNTVVIESEYPDLTAFDKEMKEFSSKPRPKPDPSKPSHTDMYVEGRREILRVW"
     gene            complement(3341..>3714)
                     /locus_tag="VFB89_00525"
     CDS             complement(3341..>3714)
                     /locus_tag="VFB89_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZM25824.1"
                     /translation="PAAGISREAAEWFLAYAWPGNARELRNAIERATLLCDGGLITLE
                     HLPIAQAATPPGTRETNGWLDPAVPLPVEGVNLDEIERILVQKALRQAKGNKSKAARL
                     LRLTRAQLYVRLQKYGLDSEA"
BASE COUNT          749 a         1227 c         1165 g          573 t
ORIGIN      
        1 ggccctgaac tcacggaagg agagaaccaa gaggaccgcc gcagccaata ccagcagggc
       61 accgcccggc gccctcagca ccaacggtac ccatggaagg gggtagagcc tttcggctgt
      121 gatcgccagg acgagcccac cgagaagaat gagtggtgga ggggcgatca cacctgcggt
      181 gtcctgggca gcgactggct ctgacatgct cccgatctca agactcggcc gttgacgaat
      241 ggccaacggc atccgcgtag cctgccaaca tgatgaccca caagcgactg cgatccttgt
      301 cgatttcgct gtctctcgtt cgtcgtacga gtagagcacg caagtcgtca gaaaccctaa
      361 ccatggagcc actcatcatg ttccgcacgt tccgcgcagc actcacggcc gcaaccgtgg
      421 ccctcgcagc cgtcactgct gtcgaagcgc aagccgcagg tcagcgcgtc aaggtgaacg
      481 ggatgcaaat gtattacgaa gtgtccggga atggcacccc cctgatcgtg ctgcacggcg
      541 cctatatgaa catccccatg atgggcacga tcatccccac cctcgccaag acgcacaagg
      601 tctacgcgct ggaactgcag gggcacggcc gtacgactga catcgaccgg cccatcacct
      661 acccaaacct tgcggacgac gtggccgcct tcatggatgc ggtgggcctg caaagtgcag
      721 acgtattcgg ctattccatg ggcgccgccg tcggactgca gctcgccatt cgccatcctg
      781 ggaaggtcaa caagcttgcc gctgcctccg tcgcgtacga cgtcgagggc tggcagccgg
      841 agttcaaggc cttcatcccg cagatgaagg tggagatgtt cctcgaaatg ccgttcgcga
      901 aggactatcg caaactggct gccaatcccg acggatttcc cgagctggtc aagaagctga
      961 tcgcgctcga gaaagagcca atggcttggg cagaacaagt gaaagcgctg aaggcccccg
     1021 tgctcatcat caccggcgac gccgatgttg cgacgctcga acattctgtc tcgctgttcc
     1081 gcatgctcgg cggcggcggc atgggtgaca tgggcaagcc gctccccgcc tcgcggctcg
     1141 ccgtaattcc cgcaacggca cacaccgccg tgatcacgca gcccgacttg ctgtacgcct
     1201 tcatcgagcc gttcctgaag gggcagacgc cgaaggggat gttcgagggc gcgaagtaag
     1261 gcccttcccg cgcgactccg ccatgatcac caccacgctg accagaacca gcagagatcc
     1321 cagcaccgtc cgcaggctca ggtcctcgcc accggcgaag tacccgagca ggacagcgac
     1381 gatcggattg acgtaggcat aggttcccac gcgggtgggt gagtccacct ggatcagcca
     1441 ggtgtaggcg gtgaacgcca cgatcgaccc ggggacgatg agataggcca gcgcgagcca
     1501 tgctttgggc gagacggtcg agggatcgaa cgcacgagcc tcgcccagcc ccgcggcgat
     1561 gagcgagagc aacacgccgc ccacgaacat ctgcgacccg gaggtcacca ccttcgaggc
     1621 cggcagcggc aatcggcgtg tcaacaccga cgccacggcc cagctcatcg ccccgatgat
     1681 caggccgaca gcctccgccc tgggcacggg tgtgccaccg aggcccaggg tccggctcgt
     1741 gagcacggcg acaccggtga tgcccatgag gagcgctatg gcgagccgca gcgtgagccg
     1801 gcgctcccgc agcacgagga tctccgaaat cgccatgaac aacgggatcg tggcgagcat
     1861 gacggcggcg attcccgacg gcaccttacg ctcggcccag aatacgagac cgtagtcgag
     1921 cacgaagatc aggaaggcga cgacgacagt ggtgagccgc tcgcgcccac ggggctgccg
     1981 ttcgcgccgc gcgagcgtcc agccccacag caacgcccct gcgagaagga atcgtatcga
     2041 cgcgcacagg aaaggcggga cctcctgaac gcaaatccgg atggccagga acgtcgatcc
     2101 ccagaccagg tagatgaccg cgtaggccag ccccttcttc catgctgtgc ccatcggcac
     2161 ggtcaggcgg gcgatccgca accggcagcg gcgcggtggc gggaaatgcg tgaagacagc
     2221 ggccggggag cgttatccgt tgtcacgctc gccactgatt cggaagacat gcccgtcggg
     2281 gtggcggacg tgcatctcgc ggacgttcca cggcatgtcc gtcgggggcc aggtgacttc
     2341 cagtccctgc tcgagacaac gccggtgaat ctcatcgacg tcatcgaccc agatcgacat
     2401 ccagacgctg cgctcggccg actggttcga ctcgggaccg aacgtcatgg gagagcccga
     2461 cgcgccgcga ccgccctggc cgtcgaggca gaggaaaatc tcgcacaccc cggaacagac
     2521 cccgccgaac gtgggcggcg atccccaatc ccacgacttc ctccagccca gcttctcgaa
     2581 ccattcgaag ctctgctgga tgtcggagac gttcagaatg gggttcaacc ggtgcacgag
     2641 catctatttc atcctccctc aaacgcgcgg gcgatcctcg caggattcct cccgattgaa
     2701 cagcatcacg aatgcggcaa tccgaagggt ccaactcctg gtccctgcgg acagatcgac
     2761 aacttgtcca tttccccgca ccgcctcccc ggggcgagca ggaaaccagg ctgcgatgcc
     2821 gcgcgaaatc gaaggtttct ccgcacccct ggcactcgga cccagccgcg gccgtgggca
     2881 cgccccttgc ggctataccg accggcacga acagggctcc gaggcggggc cgcaccgtgt
     2941 ccgttcggaa tgcatgctgt aaccgcgtag tccagaggag gtgcgggaat gatcatcgtg
     3001 cgggagatat tcatcgccaa gccgggcatg gcctcgaagc tggccaccaa gttcaaggag
     3061 gagatggcag gcgaatcatc cgggaatggg ttcaaggtcc tgaccgacgt ggtgggttcc
     3121 tacaacaccg tcgtcatcga gtccgagtat ccggacttga cggccttcga caaggagatg
     3181 aaggagttca gcagcaagcc ccggcccaag ccggaccctt cgaagccgag ccataccgac
     3241 atgtacgtgg agggcaggcg cgagatcctg cgcgtctggt aatccggcgg caaggcgaac
     3301 ggcgctcatc cacccggtga gcgccgtttt cgcagagcgg tcaggcttcc gagtcgagac
     3361 cgtacttctg cagcctcacg tagagctggg ctcgcgtcag ccgcaggagt cgcgcggcct
     3421 tggacttgtt tcccttggcc tggcgcagcg ccttctggac caggatccgc tcgatctcgt
     3481 cgaggttgac gccctcgacg ggaagcggga cggcagggtc aagccagccg ttggtctcgc
     3541 gagtgcccgg gggggtggcg gcctgagcga tgggaaggtg ctccagtgtg atcagacccc
     3601 cgtcgcacaa gagcgtcgcg cgctcgatcg cgttgcgaag ctcacgagcg ttcccgggcc
     3661 aggcgtaggc caggaaccac tccgccgcct cacgcgagat ccctgccgcc gggc
//
LOCUS       DASRJQ010000022         2156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3372088, whole genome shotgun sequence.
ACCESSION   DASRJQ010000022 DASRJQ010000000
VERSION     DASRJQ010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3372088"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VFB89_00530"
     CDS             <1..266
                     /locus_tag="VFB89_00530"
                     /inference="COORDINATES: protein motif:HMM:NF015799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HZM25825.1"
                     /translation="VHNAHVLAQTRRLADQLQTALEIRGVIDRAVGILMSRSGSTEHE
                     ALERLRSLSQHEHRKLAEVARQIVDEAVARARARNRRVENRPD"
     gene            416..1048
                     /locus_tag="VFB89_00535"
     CDS             416..1048
                     /locus_tag="VFB89_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25826.1"
                     /translation="MDLPATVLAHLHDLTASIGQDDQDLADPLTALTAALRSTASSYC
                     GFQLTIVENHWPVTLTAFTDGHDVRVGTSLGLPLALVSRFDGGSRVVFFAGTPGAFTD
                     LAADLSYALGGIPVDQAPPAVDGGDQRGTRADGHRRVIELDADLPPRSRVSGLTGLAE
                     LTVLNRGIGMLVAQGHDIEQARQALRQEAAAAGVEPHIYAGRILRRHDQS"
     gene            complement(1063..1620)
                     /locus_tag="VFB89_00540"
     CDS             complement(1063..1620)
                     /locus_tag="VFB89_00540"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HZM25827.1"
                     /translation="MSEQPQPECLQPPVRTDGPIRLAEADPAWAAQYAREEERIRVAL
                     GPRAAQVEHVGSTSVPGLAAKPAIDIMLTVPDSSDEAGYLPDLEAAGYVLQFREPDWY
                     EHRFLRDRNPDVQIHVFTVGSIEVERMLLFRDRLRTRSEERELYERTKRELAARRWDY
                     IQDYADAKSAVIAQIISRAHDGPKH"
     gene            complement(1680..>2156)
                     /locus_tag="VFB89_00545"
     CDS             complement(1680..>2156)
                     /locus_tag="VFB89_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25828.1"
                     /translation="LVAVLVMMTFLGLPRQIDIRSIDGHNRDQALAPLLHAAAQNGMP
                     IVVANRSAVRLVNAATYPQNLLGEPVDPATNKYVVVVERTRFAHTVPADFLYYQANGP
                     WRQIAKCRVSQALVRIFENDRLLTGSLGSPEEIGKRLSDPTAGAVRCQVVNRLTQK"
BASE COUNT          391 a          701 c          658 g          406 t
ORIGIN      
        1 ccgtacataa cgcccacgtc ctcgcacaga cccgacgcct agccgaccag ctgcagacgg
       61 ccctggaaat tcgcggcgtc attgaccggg ctgtcggaat cttgatgagt cgcagcggca
      121 gtaccgagca cgaggctctc gagcggttgc gttcgctgag ccagcacgag catcgcaagc
      181 tcgcggaggt agcacgccag atcgtcgatg aggccgtcgc tcgggcacga gcccgaaacc
      241 ggcgtgttga gaatcgtcct gattaggcgc cgttggcatc ccagcgtaga gtcgaattgg
      301 acgcggttat ctgactcccc ggtccgcgca gccgccgcca atggcctcgg cgatttgccg
      361 agccagttca cgcagtgacg atcagcgggc ggatccgctt cctcggagcg agagtgtgga
      421 tctcccagcg accgtacttg cgcaccttca cgatctcacg gccagcatcg gccaagatga
      481 tcaagatctg gccgacccgc tgacagcgct gaccgcggca ttgcgctcaa cggccagctc
      541 ctactgcgga ttccagttga ctattgtcga gaaccattgg ccggtcacgc tgaccgcctt
      601 cactgacggt cacgacgtac gggtcggcac gtcgctgggt ctgcccctag cgctggtttc
      661 gcggttcgat ggagggagcc gagtcgtctt cttcgccggc actcctggcg cgttcaccga
      721 tctcgccgcc gacctgagct acgccctcgg gggcatcccc gttgaccagg ctccgccggc
      781 cgtggacggg ggggatcaga gagggacacg tgctgacggt catcgaagag tgatcgagct
      841 cgacgccgat cttcccccgc gctctcgcgt atccgggctg accggcctcg ccgaactaac
      901 agtcctgaat cgcggcatcg ggatgctcgt cgctcaaggc cacgacattg aacaagctcg
      961 tcaagcgctg cgccaggagg ccgcggctgc cggggtcgaa ccgcacatct acgccgggcg
     1021 gatccttcga cggcatgatc agagctgatg tcgagtgcgc tttcagtgct tcggaccatc
     1081 gtgcgctcgg gagatgatct gtgcgatcac cgccgacttg gcgtcggcgt agtcctggat
     1141 gtagtcccac cgccgggcag caagctcacg tttggtgcgt tcatagagct cacgctcctc
     1201 ggatcgcgtg cgcagccggt cgcggaacag cagcatgcgc tccacctcaa tactcccgac
     1261 ggtgaacaca tggatctgca catccgggtt gcggtcccgc agaaaccggt gctcgtacca
     1321 atcgggctcg cgaaattgga gcacgtaacc ggcggcctcc aagtcgggta gatatccggc
     1381 ctcatcggaa gagtcgggca ccgtcagcat gatgtcgatc gccggcttcg ccgcaagccc
     1441 tggtaccgat gtcgacccga cgtgctccac ctgcgcggca cgagggccga gcgccacccg
     1501 gatgcgctcc tcctcccggg cgtactgcgc cgcccatgcc gggtcagcct cagccagtcg
     1561 gataggccca tcggtacgca caggcggctg cagacactcc ggctggggtt gctcagacat
     1621 ggcgttcagt atctccctga attgctcacc aaggatcctt ggcggtgccg cagcgtgccc
     1681 tacttctgcg tcagcctatt cacgacctgg cacctaacgg cgcccgctgt cgggtcgctc
     1741 aggcgtttcc cgatttcttc gggcgagccg aggctcccag tcagcagcct gtcgttctcg
     1801 aaaattcgta ccagcgcctg cgatacacgg catttcgcga tctgccgcca gggcccattc
     1861 gcctggtagt aaaggaagtc agcaggtacg gtatgcgcaa aacgtgtccg ctcgacgaca
     1921 accacgtatt tattggtagc aggatccacc ggctcgccca gcagattctg ggggtatgtg
     1981 gctgcgttga caagtctcac tgctgaccta ttggcaacaa cgatcggcat tccattttgc
     2041 gctgcagcgt gcaagagcgg tgccagtgcc tgatcacgat tgtggccatc gatggatcta
     2101 atatcgattt ggcgtgggag gcccaaaaac gtcatcatga cgaggaccgc gaccag
//
LOCUS       DASRJQ010000023         2833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2524745, whole genome shotgun sequence.
ACCESSION   DASRJQ010000023 DASRJQ010000000
VERSION     DASRJQ010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2524745"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..602)
                     /locus_tag="VFB89_00550"
                     /pseudo
     CDS             complement(1..602)
                     /locus_tag="VFB89_00550"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(932..1354)
                     /locus_tag="VFB89_00555"
     CDS             complement(932..1354)
                     /locus_tag="VFB89_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25829.1"
                     /translation="MIVRYPAVAAITLLMGAGCAHYTPAPLDPAATAQAYNQRRLDAP
                     ELTEWLQVGQGEQHEPLLLASTALDSSRLDNCCFSFDRLWLPQSRPSAITIEYLGHSL
                     KSRKKRPELMMEAEGADRLPALSETLAAREPHALVGRR"
     gene            complement(1351..2265)
                     /gene="xerD"
                     /locus_tag="VFB89_00560"
     CDS             complement(1351..2265)
                     /gene="xerD"
                     /locus_tag="VFB89_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HZM25830.1"
                     /translation="MTPVVERIDRVFEIESFRDHLALEAGNSANTVEAYVRDIRRLAE
                     FATSKGATEPAQVTRTLLRDFVFLLKDLGLSSATIRREISAVRTYFAFLLGEGRITDD
                     PSDRLESPQRWRHLPEVLTVGEIESLLAAPHVDEPMAWRDRALLELAYGVGLRVSELC
                     NLGLSDLVLSEGLVRVFGKGSKERLVPIGRSVIGAVSTYLHTLRPSLDRGKTEGRLLL
                     NARGEPLSRVGAWGVVKRCAKRAAISKRVTPHTLRHSFATHLLEGGADLRAVQEMLGH
                     ADLSTTQIYTHVDREYLHSVHKQYHPRA"
     gene            complement(2262..>2833)
                     /locus_tag="VFB89_00565"
     CDS             complement(2262..>2833)
                     /locus_tag="VFB89_00565"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HZM25831.1"
                     /translation="ANWERITSVLSGVNRTLGWVALIAVLAWLTTTLMRRRRRRRERV
                     LEAADRALGERLPTGEHPVIDPRAAAHLVIELAYADEALTQQDRHLVERHLRARWGLE
                     PIPHEPPLAARLQSRFEQYRERLTGRFGRAQRLALIEGMWQAAFSDGAMDAQEDRLMQ
                     RAGDLLGLSPAEVAEVRRRSRAGAEHPAR"
BASE COUNT          544 a          922 c          888 g          479 t
ORIGIN      
        1 cgaagactgg aaagaccaat gccgtcatcg tcctcatcga gacggcgctt agtgtcgatc
       61 ttgggcttgg gaaggaatag cgacagcgcg aaagcgaggg caccgatcgc agctgctgcc
      121 acgaagatca tggcgaagtc agctttcagg acaatggccc cgcccacaat cgggctgagc
      181 acatagcctg cgcccttgat gccgttgtag atgccatagg cccgacctcg ctgctcgacg
      241 gcgtatgctc ctacgagagc cagcgacaca gccgatagcg ccgctgctca aacaccttgc
      301 agaaaacgga tgaggagcaa gagacgcggg tctacccaca ggtaagccag agaagctatg
      361 acgaacaatg ccagaccggc gagcacggtc tgtttcattc ctcgccgatc ggcgatcgct
      421 ccgaacacgg gcttcgcaac aacttctgca gcgtcatatg ctgcgatcag gagaccaatc
      481 actgccacgc cgacaccgac ttgggcggca tacgtgggca ggttaacggc gacgacgtgc
      541 gcgccgaacg atgtgacaaa ggcgacgaaa cctaggatgc tgattggcca cagtgctgac
      601 actatgctct tggatgacaa gatggcgact taaccatttt gcccctcagc tgaagccagg
      661 gcgcgcctgg acatccgact tttgcgttcc actgatcgcc ggcgacggga gcagcccccg
      721 gccgagctcg ggcgcaccgc gcccgtcagc tcccatgcgc tggttgcgcg gtgcagctca
      781 accggttaca cgcttgtgcg agctggaagg ggacgtgacc ttctgggcat agcccaagca
      841 tagcacagac gtgtccgtgc tctcgtgcaa tttgatccgc tccaccgctt gctgctaact
      901 cagcccgccg atcttccggg tccgatacca atcaacggcg cccaacgagc gcatggggtt
      961 cacgggccgc caaggtctca gacagagctg gcaagcgatc tgccccctca gcctccatca
     1021 tcaattcggg gcgtttcttc cgacttttca gcgagtggcc gaggtactcg atcgtgatgg
     1081 cgctcgggcg agattgcgga agccagagcc ggtcgaaact gaagcagcag ttatccaggc
     1141 ggctcgaatc caacgcggta ctggcgagga gcagtggttc atgctgctcg ccttgcccga
     1201 cctgcaacca ttcggtcagc tcaggagcat ccagccgacg ttggttgtag gcctgagcag
     1261 tggctgctgg atcgagcggg gccggcgtgt agtgagcaca gccagccccc atcagtagcg
     1321 taatggcggc gacagcagga taccgcacga tcacgcccgc ggatggtact gtttgtgaac
     1381 cgagtggaga tactcccgat ccacatgcgt ataaatctgg gtcgtcgaaa gatcggcatg
     1441 cccgagcatc tcctgtaccg cgcggagatc ggccccgcct tccaggaggt gagtggcgaa
     1501 cgagtgccgg agtgtatggg gcgtgacccg cttgctaatg gcggcgcgct tggcgcaacg
     1561 cttcacgacg ccccaggcac cgacccggga gagtggctcg ccccgcgcgt tcaacagcag
     1621 ccggccctcc gtctttcccc gatccaacga ggggcgaagc gtgtggagat acgtcgaaac
     1681 ggccccgatc acgcttcggc cgatgggcac caggcgctcc ttgcttcctt tgccgaagac
     1741 acggaccagc ccctcgctca ggaccaggtc gctgaggccc aggttgcaaa gctccgaaac
     1801 ccgcaaaccg acaccatagg caagctcgag cagcgcgcga tcccgccagg ccatgggctc
     1861 gtccacatgc ggtgcggcaa gaaggctttc gatttcgccg accgtaagca cctccgggag
     1921 gtggcgccat cgctgaggac tctccagccg gtcgctcgga tcgtcggtga tccggccctc
     1981 cccgagcagg aaagcgaaat aagtgcgaac tgcggagatc tcccggcgga tggtcgcgga
     2041 gctcaagccg aggtccttga gcaggaagac aaaatcgcgg agcagggtcc gggtaacctg
     2101 cgccggctcg gtggcgccct tggacgtggc gaattccgcc agccggcgta tgtcgcgaac
     2161 atacgcctcg actgtgttcg cgctgtttcc tgcctcgagt gcgaggtggt cgcggaacga
     2221 ctcgatctcg aagacccggt cgatgcgttc gaccaccggg gtcatcgggc cggatgctcc
     2281 gccccggccc gagaccggcg ccggacttcc gcgacctcgg cagggctcag ccccagtaga
     2341 tcgccggccc gctgcatgag gcgatcctcc tgtgcgtcca tggctccgtc actgaacgcc
     2401 gcctgccaca tgccttcgat cagggcgagt cgctgggccc gaccaaaccg acccgtcagc
     2461 cgttcccgat actgctcgaa gcggctctgc aatcgggcgg cgagcggagg ttcgtggggg
     2521 atcggctcga gcccccaccg agcccgcagg tggcgttcga ccagatgccg atcctgctgc
     2581 gttagtgcct cgtcggcgta ggcaagctcg atcaccaggt gggcagccgc ccgtggatcg
     2641 atgaccgggt gctctccggt cggcaggcgc tcgcctaatg cccggtccgc agcctccagc
     2701 acccgctccc gccgccgccg gcgacgccgc atcagtgttg tagtgagcca ggccaggacc
     2761 gcgatgagcg cgacccaacc gagtgtgcgg ttcacacccg agagtacgct cgtgatccgt
     2821 tcccagttgg cgc
//
LOCUS       DASRJQ010000024         2974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1958004, whole genome shotgun sequence.
ACCESSION   DASRJQ010000024 DASRJQ010000000
VERSION     DASRJQ010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1958004"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="VFB89_00570"
     CDS             complement(<1..904)
                     /locus_tag="VFB89_00570"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZM25832.1"
                     /translation="MTTSAPPLVSIDAVQAAFGTQGYIADRDLATAIFLSLTLGRPLL
                     LEGEAGVGKTDVARTLSLALGAELIRLQCYEGIDLSTAVYEWDYPRQMLEIRLLEARG
                     EAKSAATRDIFSEEFLIRRPLLRALEPKPVPPVLLIDEVDRADEEFEAFLLELLSDFA
                     VTVPEIGTLRAERAPRVVLTSNRTREVHDALKRRCLYHWIDYPTPERELAILHARLPH
                     TPENLARDVVAFVQRLRSADLTKVPGIAETLDWAAALVALDAQQLSAEQVDQSLGVLL
                     KYQEDLAIMRGGAVRAMLEESRAVR"
     gene            complement(901..1977)
                     /locus_tag="VFB89_00575"
     CDS             complement(901..1977)
                     /locus_tag="VFB89_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25833.1"
                     /translation="MRAARLSPSGLARSTPKELTGAILLEPVRAPGCSMAKGQSLDQD
                     AAARLVAAAATGMLTRLVKIAWPEAGDVHEDEAAAQLARAVGGAGVDLRAPRQSRFDL
                     AAMWDGVLHLRIEPLHRLNAIDPLEVFTLFHGQAVAQGQVVCSVKVAPHLLPAATLAE
                     GLRTARTDGPLVEVRPYRDMKVGAIAVQAITPDALERFEQGARRKVEALGGTFMGVVV
                     VPDEDPDKAEKGATEALRALVSEQSVPLLLVGGVSAGDPLSPFYAALQRRGGKVVRRG
                     VPAHPGSMIWLAQLEHTQILGLPQCGMFSLATAADLVLPRLLTGEVLTAEALADLAHG
                     GLLGREMRFRMPAYARELPAPEEP"
     gene            complement(1979..2434)
                     /locus_tag="VFB89_00580"
     CDS             complement(1979..2434)
                     /locus_tag="VFB89_00580"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HZM25834.1"
                     /translation="MKLHFEGAPEIVTSRAHAWRCLMDPNFVAQSAPGVESVTTIDPT
                     HFTVVSAFGVGAIKVRFSLNVELTDVVEPDHATMRARGKAPGSNVDVVANLRLEDAAN
                     GRVRLNWAADSDVSGTVASVGARLLEGTARKLTEEFWTDFARRVQADAS"
     gene            complement(2431..>2974)
                     /locus_tag="VFB89_00585"
     CDS             complement(2431..>2974)
                     /locus_tag="VFB89_00585"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HZM25835.1"
                     /translation="RGRPLLGHVLGALRPSLESGLLAGGCVVVPADSPAVAALASEAG
                     MVPLPNHAPALGLSFSLGIAFDWLLPQAHRLPAAAVIFLGDQPAVPPDVLPNLIAAWR
                     RGDGMVIRPRYAQAPSEPGHPLLVDQSLWGLANELTGDAGFGLVFSARQIPIVTIETP
                     GLNPDVDTPTDLTNLEGIEQ"
BASE COUNT          522 a          988 c          973 g          491 t
ORIGIN      
        1 gcctgactgc tcggctctct tccagcatcg ctctcacggc tccgccgcgc atgatcgcca
       61 gatcctcctg gtacttgagg aggacgccga gcgactgatc gacctgttcc gcactgagct
      121 gctgcgcatc gagtgcgact aatgcggcgg cccagtccag cgtctcggcg atgccgggaa
      181 ccttggtgag atccgccgat cggagtcgct gcacaaacgc gacgacgtcg cgcgccagat
      241 tctccggtgt atggggcaac cgtgcgtgca ggatggcaag ctcccgttct ggggtcgggt
      301 aatcgatcca gtgatatagg cagcgtcgct tgagggcgtc gtgcacctcg cgcgttcggt
      361 tgctggtgag caccacccgc ggtgcccgct ccgctcgaag ggtaccgatc tcgggaaccg
      421 tcactgcaaa gtcgctcagg agctcgagca agaaggcctc gaactcttcg tcggcccggt
      481 cgacctcgtc gatcaacagc acgggcggta ccggcttggg ctcgagcgcg cggagcaagg
      541 gccgacgaat cagaaactcc tcgctgaaga tgtcgcgggt cgcggcggac ttcgcttcgc
      601 cgcgggcttc cagcaggcgg atctccagca tctgtcgcgg atagtcccat tcatacactg
      661 cggtgctcag gtcgatgcct tcgtagcact ggagccggat caactcggcg cccagcgcga
      721 gagacagcgt tcgggctaca tcggttttgc cgacgccggc ttcaccctcg agcaacagcg
      781 gtcgtcccag agtcagtgac aggaagatcg cggtcgccag gtcccggtcc gcgatgtacc
      841 cctgggtgcc gaaggcagcc tgaacggcat cgatgctgac gagtggtggg gcgctggtgg
      901 tcacggctct tccggggccg gaagctctct cgcgtaggcc ggcatgcgga accgcatctc
      961 tcttcccagg agcccaccgt gagccagatc tgccagtgct tccgcggtga gcacttcgcc
     1021 ggtgagcagt cgaggcaaca ccaggtcggc ggcagtggcc agactgaaca tgccgcattg
     1081 gggaaggccg aggatctggg tgtgctcgag ctgagccagc cagatcatcg agccggggtg
     1141 ggctggaacg ccacgccgca cgactttccc gccccgccgt tggagcgccg catagaacgg
     1201 agataacgga tcgccggccg agacgccgcc caccagcaat agtgggacag actgctcgga
     1261 aaccagtgct ctcaaggcct ccgtcgcgcc cttctcggcc ttgtccgggt cctcgtccgg
     1321 caccaccacc actcccatga acgttccacc cagcgcttcg acctttcgcc gtgccccttg
     1381 ctcgaagcgc tccagcgcgt ccggtgtaat cgcctgaaca gcgatcgccc ccaccttcat
     1441 gtcgcgatac ggccgcacct ccacaagcgg accgtccgtc cgggcagtgc gaagtccctc
     1501 agcgagtgta gcagccggca gcaggtgcgg tgcgactttt accgagcaga ccacctgtcc
     1561 ctgcgccacc gcctggccgt gaaaaagggt gaacacttcg agcgggtcga ttgcgttgag
     1621 ccgatggagt ggctcgatcc gaaggtgcag cacgccgtcc cacatcgctg ccagatcgaa
     1681 gcggctctgt cgcggtgcgc gcaagtcgac ccctgctccg ccaaccgcgc gggctagttg
     1741 agcggcggcc tcatcctcgt gcacatcacc ggcttcgggc catgcaatct tgacgagtcg
     1801 ggtgagcatg ccggttgccg cggcggcgac tagtcgggcc gccgcgtcct gatcgagcga
     1861 ttgtcccttg gccatggagc agccgggcgc tcgcaccggt tcgagcagga tcgctcccgt
     1921 gagctccttg ggcgtcgaac gggcgagccc ggaagggcta agccgggcag cgcgcacgtc
     1981 aactcgcgtc cgcctgaacc cggcgggcga aatcggtcca gaactcttca gtgagcttgc
     2041 gcgccgtgcc ttccagcagc cgagcgccca cactcgcgac ggttccgctc acatcactat
     2101 cggccgccca gttgagccgc acccgaccgt tggcggcgtc ctccagtcga agattggcga
     2161 ctacgtcgac gttcgagccg ggcgccttgc cgcgcgcgcg catggtggcg tggtccggtt
     2221 ccaccacgtc ggtcagctcg acgttcaagc tgaaccgcac cttgatcgcg ccgacgccga
     2281 acgccgaaac gaccgtgaag tgcgtcgggt cgatcgtagt gaccgactcg acgccggggg
     2341 cagactgagc gacgaagttg gggtccatga ggcagcgcca ggcgtgagcg cgactcgtaa
     2401 cgatctccgg tgcgccttcg aaatgcagct tcattgctcg atgccctcga gattcgtgag
     2461 gtcggtcgga gtgtccacat ccggattgag gccgggcgtc tcgatggtaa cgatggggat
     2521 ttgccgagcc gagaatacca gcccgaaccc ggcatcgccg gtcaactcat tcgcgaggcc
     2581 ccagagcgac tggtctacca gcagcggatg tcccggttcc gacggagctt gggcataccg
     2641 gggccggatt accataccat ccccgcggcg ccacgcggcg atgaggttgg gcagtacatc
     2701 cgggggaacc gccggttgat cgccgagaaa aatgacggca gcggcgggaa gccgatgagc
     2761 ctggggaagc agccagtcaa aggcgatccc gagagagaag ctgaggccca acgcgggagc
     2821 gtgattcggc agggggacca tgccggcttc gctggccaga gccgccacgg ccggcgaatc
     2881 cgctggaacg accacacacc ctcccgcgag caacccgctc tcgaggctcg gtcgaagtgc
     2941 gccgagcaca tgtccgagaa gcggacgtcc gcgc
//
LOCUS       DASRJQ010000025        13019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2788480, whole genome shotgun sequence.
ACCESSION   DASRJQ010000025 DASRJQ010000000
VERSION     DASRJQ010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 13019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13019
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2788480"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="VFB89_00590"
     CDS             complement(<1..712)
                     /locus_tag="VFB89_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25836.1"
                     /translation="MDAGSLYSSPNALAPDYSRFRVGERLLLTGHSHQAWPDVGFDAQ
                     AAAWNDAAEFVDDKWDHAFAQADRVREGFTWLLGDAGGGIALAPNTHELVVRLISALP
                     LRSRPRLVSTDGEFHTIRRQLDRLAEEGLEIVRVAESPLESVGARLADAVDGRTSLVL
                     VSLVFFDTGRIARGLAHVAAACRRHGAALLVDAYHALNVVPVSLADLGLDDAFVVGGG
                     YKYRQLGEGNCFLRIPKDT"
     gene            complement(735..1514)
                     /locus_tag="VFB89_00595"
     CDS             complement(735..1514)
                     /locus_tag="VFB89_00595"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HZM25837.1"
                     /translation="MPSYGDYLKLDELLALQRPLSKGPEHDEMLFIVIHQVYELWFKE
                     ILHELDYLNGLLVRNDGARARHTLKRILTVLKVMVAQLDILETMTPLEFLSFRDRLES
                     GSGFQSHQFRELEFALGWKDHTALERYATGTEGRRRLEKRLAASTLWDAFLRHLAANS
                     VPVPRTDLERDVTQKVVPSAALRDVLVKVYRERPDLAELCERMVDLDEGLMEWRYRHV
                     KMVQRTIGTRRGTGGSAGAEYLVSTLNQPLFPDLWTIRTEL"
     gene            complement(1825..2409)
                     /locus_tag="VFB89_00600"
     CDS             complement(1825..2409)
                     /locus_tag="VFB89_00600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HZM25838.1"
                     /translation="MRSWSGVIAAIAAVGVFAGSAAAEARPTGGWNETTIGRLGPLGT
                     SNSLFRSAVSGYSDGVAHVSKRDGNSSDDTDDNKGGISTTALIVGGGAAVGIGVFIAA
                     VSSGGSSDLSTTPHSPGSVDNPTGGGNGNTGGGDGLVPSTGGGGSTGGTGSAGGDPST
                     VTPEPASMALLASGLAGMGGFQLRRHRRRIQEEK"
     gene            2724..2797
                     /locus_tag="VFB89_00605"
     tRNA            2724..2797
                     /locus_tag="VFB89_00605"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2758..2760,aa:Val,seq:cac)
     gene            3509..4969
                     /locus_tag="VFB89_00610"
     CDS             3509..4969
                     /locus_tag="VFB89_00610"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HZM25839.1"
                     /translation="MERRSFLGGITLAALSRHAGTVEDNTSRVFDIKQYGAAGDGRTD
                     DTIAIRRALAELNKAGGILSLPPGDYVVSAPLDLPTNAAWHLRGAGRTVTRLRLGGSA
                     SRCDLFHCAHGHTRPTFGCSMQDLTIDAGGFPGTTLRLDGLTLFGMRSVTIARANGTA
                     LGLVGLFDSYFEDVFLEGCGAASEPALSCTSQMDGRGQSMNNCVFVNVHIESGADAVH
                     VFIDGSQNNPTDTLQFYGLKCHGQPQTGMPARPLLKLGRYAIGCSFTGGLIAWGKGAS
                     QVEVDGRANKFIGIDHGAGPPGGSPEFAYRFTEHAVGNHILTPNFKNGVGPNQYRSGY
                     VRVDAGASNTKLLFPQMSTGPLAIDRVLSDEGRATLFLGDDISDARGLYLRHSQGFNV
                     LRANGISTTGTPAWNLRGSVAISGGSDKADVRFQTPEADAGYFVTCAVTGVKGSPAAA
                     SRRVSISDKNEKGFRVRAEEAPGGNNAVMVDWILVR"
     gene            complement(4941..6485)
                     /locus_tag="VFB89_00615"
     CDS             complement(4941..6485)
                     /locus_tag="VFB89_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25840.1"
                     /translation="MSSTRRAFVGAALVLLAGVRSARAQASPYLPLDDSRLPLIEHLI
                     ARGDVVDPSPMIRPFRYADAIKVLSAADTAPETTSGRLVHRLNESLRSDVRSDKSWWR
                     VRLRGGGEAYTQKRRDPLHLGGPGTANPYGDFELWGLFGPVAGVVRTSIEPSLIGNPD
                     WPNKAQENVSGRLIEGYLSGQIKWGALTYGQLLHNWGPVGVPGIPLSDVAFERQGLEL
                     LLGTGIVRLRALASDLRPQANAAGQSVNRYYFVHRLELQVSRRLLLVPWESVLIQGVG
                     RTLETPFANPLSPTVLANNFGIADTGSNTIIGFDVSWRLGRRVTLQTQTAIDDFWFNK
                     RNQKQDRYAFTVAGYGPIGSRLGWRAWYTLVSSLALRTQNPQENFTDQGVGIGRNFSD
                     DDQLSVSVTVPAARQWLLTPELTFQRQGEGKINDPYPPPDANGNQITPMFLIGTVEKT
                     YRVALGVAGREGPVELAGNAGFHHVTNDQNQLGVTANRFVARIKVTLAWGRSGSLASG
                     PESSPP"
     gene            6613..7257
                     /locus_tag="VFB89_00620"
     CDS             6613..7257
                     /locus_tag="VFB89_00620"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZM25841.1"
                     /translation="MTAKQDNLDPQTRDLVRFAAAIAQGYEPELRERVGPLRSSQVPV
                     SWVEELILQSVLMCGYPRALVAFMVWRKFGAVPAPQDDPEARYDNVAAWTARGEETCA
                     LIYGDNYLKLRDSVRVLHPALDGWMITEGYGRTLARPGLDLTRRELCTVAQTAVLETH
                     RQLHSHLRGALNAGATFGQIDGVLSIVNPLLSFDQWKKIKELWRTVREGWSPGQ"
     gene            7254..8303
                     /gene="obgE"
                     /locus_tag="VFB89_00625"
     CDS             7254..8303
                     /gene="obgE"
                     /locus_tag="VFB89_00625"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HZM25842.1"
                     /translation="MSFVDRAAVKVVAGTGGSGASSFARFKYKPKGGPDGGDGGRGGS
                     VYVRADPNLSTLLDYRYRTLWEADRGEHGKGKTQTGRSGEDIYLPVPPGTEIRDAKTG
                     SPLGEVLRGGDTVLVARGGRGGRGNARFATPTHQAPREWEPGEEGQEREIELILKLIA
                     DVGLVGEPNAGKSTLLSVISAARPKIADYPFTTLEPNLGVMELSDHRSLVVADIPGIV
                     EGAHAGKGLGLRFLQHVERTRVLALLIPLDAGDPGAVYDRLRNEIRRYSGALYEKPHL
                     IVLTKRDLLASDGPMPPVRAADGVDVLAISSVAGTRLEELKETLWAFVSKAREDERRR
                     EDAWTKSELPTSPWP"
     gene            8264..9367
                     /gene="dprA"
                     /locus_tag="VFB89_00630"
     CDS             8264..9367
                     /gene="dprA"
                     /locus_tag="VFB89_00630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HZM25843.1"
                     /translation="MDQERAAYVALALTSGIGAARMANLLSACGSALGAFSAPFAFLR
                     SVPGMNAAAATAIRNMSVSDGERVLAEVQSLGGETILPSDACYPASFRPIEEPPIVLF
                     AAGNLGLLGRCAVAIVGSRNHTAYGAAACHMVASAFARAGGSVVSGMARGLDAVAHQT
                     ALDAGGGTIGVLGNGLGVVYPAANRRLYDAMVDRGLLLTEFPPGERPHAGSFPQRNRL
                     ISGLARATVVVEAAPGSGALITANCALEQGRELLALPGPITSATSVGTNRLIRDGATP
                     VVDPADLFHHYPELEIAGGWTSWPDAARSSPSLQLPPDAVALVEAVGFDPVHIDTLAE
                     RLGLPPGELLGSLCTLELAGVVVQQAGGMFRRA"
     gene            9388..10485
                     /gene="hemW"
                     /locus_tag="VFB89_00635"
     CDS             9388..10485
                     /gene="hemW"
                     /locus_tag="VFB89_00635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HZM25844.1"
                     /translation="MHVYFHIPFCARRCSYCDFAIAVRREVPSRAYVEAVLRDWAARL
                     NEPPWDREAIVETIYFGGGTPSRLEPHAIEQLLDRIRSDRTVALGAEITLEANPDDVS
                     RTSAAAWRGAGVNRLSLGAQSFSVDALRWMHRTHDPHAIGQAVEAARASGFDNISLDL
                     IFALPDTIQRNWAHDLDQALTLAPEHVSLYGLTTEPGTPLARWSASGRVRPTDDTRYA
                     DEYLLAHERLSTAGFEHYEISNAGRPGYRSRHNSAYWTRAPYLGVGPSAHSGHGNRRE
                     WNIREWTAYERTARAGAELIEGREALDQAAVRLEEIYLGLRTLEGVPRDWIPESKSAE
                     WRRRDWALVSDGRLRLTVEGWLRLDALVASL"
     gene            10530..11567
                     /gene="hrcA"
                     /locus_tag="VFB89_00640"
     CDS             10530..11567
                     /gene="hrcA"
                     /locus_tag="VFB89_00640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HZM25845.1"
                     /translation="MSAQDQLSERELRVLEAVVQTYIETAEPAGSQTIARRFGLGVSP
                     ATIRNTMSELEEKGYLFHPHTSAGRVPTDRAYRLYVDGLIRLAPPSLEESKALRAEVL
                     GSGSTLEEILRRAAQVLGVLTQELGVAVAPALDELVLERLDLIPVTSDRVVLVFNLQS
                     GVVRTIFVQVPSNLPGPSVQRVTQILNDRLSGLTLAQIRQSLVERLRDADHAPDSREL
                     LNIFIAERDDIFDLSGNGATVMLGSAQMLADQPEFASNLGMRGLLELTERRDLLRRAL
                     EARRRAGLSITIGWENLDPRLSNFTLVTSSYQAGELKGVIGVMGPTRMPYEKIIGLVE
                     HTSRLVEDLLE"
     gene            11564..12697
                     /gene="dnaJ"
                     /locus_tag="VFB89_00645"
     CDS             11564..12697
                     /gene="dnaJ"
                     /locus_tag="VFB89_00645"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HZM25846.1"
                     /translation="MNEFYELLGVSRTASEADIKKAYRKLAMDYHPDRNKSAEAEAKF
                     KEIAEAYEVLRDPQKRAAYDRYGRAGVSGAGAGGFGFHHVDLSEALEIFMRDFGGLGG
                     LESIFGGGRAQAESRRGQDIRVTVRLTLAEVATGTKKSVKLKTLERCENCAGSGARPG
                     TKPSVCSTCGGSGEVRRTARSMFGQFMSVSPCPTCGGDGMVVLEPCEVCRGDGRVRGE
                     RTVPVEIPAGVSANNYLTLRGQGAAGPRNGPNGDLLVMLDIKEDERFERQGNDLLFDL
                     PLSFSQAALGGEFQVPTPHGEERVRVPAGTQTNTVLRLKGKGLPALGQSGSGDFMIRI
                     HVWTPERLSDEQERLFRELAKHEGEPPRRAPGFWTKLKEALGA"
     gene            12694..>13019
                     /locus_tag="VFB89_00650"
     CDS             12694..>13019
                     /locus_tag="VFB89_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25847.1"
                     /translation="MKWWAVDVLISPERRESVGAWLVARTGHAVEERADGTLVGFAED
                     EAAAERLSVAVADEAGVPVQVSCRPLETVDWRDRWRDGLSPRRIGRLTVTPSWAALDG
                     LPSEPVL"
BASE COUNT         2319 a         4112 c         4246 g         2342 t
ORIGIN      
        1 cggtgtcctt ggggattcga aggaagcagt ttccctcacc cagttggcgg tacttgtagc
       61 cacctccaac gacgaacgcg tcgtccagtc ccagatcggc gagcgagacc ggcactacgt
      121 tcagcgcatg gtaggcatcc accagcagcg cggcaccgtg gcggcgacat gcggcagcca
      181 cgtgggctag ccctcgcgcg atccgcccgg tatcgaaaaa cacaagcgag accagaacca
      241 gcgaggtccg tccatcgacc gcgtcggcca gtcgagcgcc caccgattcg agcgggctct
      301 ctgcgactct gacgatctcg aggccctctt ctgccagacg atccagctgc cgtcggatag
      361 tgtgaaattc gccgtcggtg ctgaccaatc gcgggcggct tcgaagcggg agggcggaga
      421 tgagacgtac caccagctcg tgggtgttgg gggccagcgc gatgccgccc ccggcatctc
      481 ccaggagcca agtaaatccc tcacgcacgc ggtcggcctg agcaaacgcg tggtcccatt
      541 tgtcgtccac gaactccgcc gcatcgttcc aggccgctgc ttgggcatcg aaacccacat
      601 ccggccacgc ctggtgcgaa tgccccgtca ggaggagccg ctcgcctacc cggaagcggg
      661 aataatcggg tgccagcgca ttgggcgagc tgtagaggga gccagcgtcc attgagcccg
      721 gcgggaaagg gtgactagag ctccgtgcga atggtccaga gatccgggaa cagcggctgg
      781 ttgagcgtgc tgacgaggta ctcggcgccc gcactcccgc ccgtcccgcg ccgcgtgccg
      841 atggtgcgct ggaccatctt cacgtgtcga tagcgccact ccatcagccc ctcatccagg
      901 tcgaccatgc gttcgcacaa ttccgcgaga tcaggccgct cccggtagac cttgacgagc
      961 acatcgcgca atgccgcgga gggaacgacc ttctgggtca cgtcgcgctc gaggtcagtc
     1021 cgggggacag gaacgctatt ggccgcgaga tggcgcagaa atgcatccca tagcgtgctc
     1081 gcggcaaggc gcttctccag ccgacgccga ccctcggtgc cggtggcata acgctccagc
     1141 gccgtatgat ccttccaacc cagggcgaac tcgagctcgc gaaactggtg cgactggaag
     1201 ccgctgccgg attcgaggcg atcacggaag gaaagaaact cgagtggtgt catggtctcg
     1261 agaatatcca gctgtgccac catgactttg agcacggtga ggatccgttt gagcgtgtgg
     1321 cgtgcgcgcg cgccatcatt ccgcaccagc agcccgttga gataatcgag ctcgtggagg
     1381 atttccttga accagagctc atacacctga tgaatcacga tgaacagcat ctcgtcgtgc
     1441 tcaggcccct tcgacaaggg ccgttgcagg gcgaggagtt cgtcgagctt cagatagtcg
     1501 ccgtacgagg gcatgggatc tcggggccgg tggagcttcg gcgtcgccga ggagcgtagc
     1561 tgtcccgagg cgctgacgga agggtcaggt cacgctcttt cccggccgcc agtctgattg
     1621 tagcgcgctc actgctactg cagcctcggt cgttagtgga aagcgcagct cgcgccgctg
     1681 gtcgcgctca ctatggcccc gggagccgcc gactttttgc gggttcctct cgcggctgcc
     1741 tgaccgcttc tgctcaccga caatacaaaa gcagaacgcc cctcttacga ggggcgtccg
     1801 cttcgttcgg tgttccccgg ggttttactt ctcttcctga atccgccggc ggtgccgtcg
     1861 gagctggaag ccacccatcc cggccaggcc agaagccagc agtgccatgg atgcgggttc
     1921 cggcgtaacg gtggaaggat ctccgcccgc cgagccggtg ccgccagtcg atccaccgcc
     1981 cccggtggac gggaccaggc catcgccacc gcccgtgtta ccgttgcccc cgccagtggg
     2041 gttgtcaacc gagcccggag aatggggagt cgtggagagg tccgacgacc caccactgct
     2101 caccgcggcg atgaacacgc cgatgccgac agccgcgcca ccaccgacga tcagcgccgt
     2161 ggtgctgatg ccacccttgt tgtcatcggt gtcatccgag ctgttcccat cacgcttgga
     2221 gacgtgggcc acgccgtcgg agtatccgga aacggcagag cggaacaggg agttgctggt
     2281 ccccaacggc cccagccgac caatggtggt ttcattccat ccgcccgtcg ggcgagcttc
     2341 ggcggcggca gagcccgcga atacgccgac ggctgcgatt gccgcgatta caccagacca
     2401 gcttctcata aggtcactcg gggtaagagg acacacagaa cgcggcgact gtttttgcaa
     2461 ctcataggcc atgtgaccga ttcagccatg ccgtatctgt taacctctga gacagtaata
     2521 ttttaggcag ttcgtcccca ccgagtcggt ccccagaatg gagctaaatc actgtgggac
     2581 tttggcctcc gcgcatcggt tttgtgtgga ggcactccca caatctccct cctcaaagag
     2641 ctctgtcaag tatcccggtc acacagtcta tcctgcgccc ttgtcgtggc ttggaccgct
     2701 cgttactatg ccgcgctctt cgcgggcgat tagctcagct ggttagagcg cgcgcctcac
     2761 atgcgcgagg tcgggggttc gagcccctca tcgcccatct cagtcctcac ggccccgcac
     2821 cagagtgcgg ggattttgtt gggcgcggct cacttcttta cagacttcgc ccccaacgat
     2881 cgaaccagat cccgattcaa ttcagcttca ccgctcgcca gactccgcca ggaaagttcg
     2941 cgtcccttcc gcttgccctt cggcgcgatg tgcacgcccg tgggcccgat acttacagtg
     3001 tagggctcac catccaattc aatctctcgt ttgatcgtct cggttaatcg cggggccata
     3061 gtcggaacgg ttcggagtag gcgtttggga cgaatgtgac gcctgaccgc aggtcgtcaa
     3121 ggtggagatg tggacgacgc aaagacaccc ggcggcattc tcgagcaagc gtttggctca
     3181 gcagcgaaga gccgactccg tgacgggagg ggtgagcgtc ggcaacgatg gcccgtgctc
     3241 tgcgtatacc atgctatgaa ctactcgcaa acgcagtggc actccgtagc taagctctgc
     3301 cgtctcaacg aagaacgtgc gtctacggcc gtccaagccg agccgcaggc ctgcgccgca
     3361 ctctgcggga ttcactgctg agtatgcagt catgcacaat cgatgaggct cgcggagccg
     3421 ggaaaggctc tggtctcgcg ggcgctactc gccgcccgtt gccccctgag gttttcaaca
     3481 acccagtccg cccttgccgg agggcagcat ggaacgccgt tcctttctgg gaggaataac
     3541 cctcgccgcc ctgagtcgtc atgccggaac cgtcgaggac aacacctcgc gtgttttcga
     3601 catcaagcag tacggggcag ccggtgatgg ccggaccgac gataccatcg cgatccgtcg
     3661 agcgctcgcg gagctgaata aggcaggcgg gatcctgtcg ctccctccag gcgactacgt
     3721 cgtgtcagcg ccgctcgatc tgcctaccaa cgcggcctgg caccttagag gcgcgggccg
     3781 aacggtcact cgactgcgtc tgggaggatc cgcgtcgcgc tgcgacctgt tccactgcgc
     3841 ccacggtcac acccggccca catttggctg cagcatgcag gatctcacca tcgatgccgg
     3901 cggctttccg ggcacgacac tgcgacttga cggactgacg ttgttcggga tgcggagcgt
     3961 caccatcgcg cgcgcaaacg gcaccgcgtt gggcctggtc ggattgttcg actcgtattt
     4021 cgaggatgtg ttcctggaag gatgtggggc tgcgtcggaa cctgccttga gctgtacctc
     4081 gcagatggac gggcggggcc agtccatgaa caactgcgtc ttcgtcaatg ttcatatcga
     4141 atccggcgca gacgccgtgc atgtcttcat cgacggaagc cagaacaatc ccaccgacac
     4201 gctgcagttc tatgggctca agtgccacgg tcagccgcaa acggggatgc cagcgcgccc
     4261 gctgctcaaa ctgggccggt atgcgatcgg ctgttccttc acagggggcc tcatcgcctg
     4321 gggcaaggga gcgtcgcagg tcgaggtgga cgggcgagcc aacaagttca tcgggatcga
     4381 ccacggtgct ggaccgccag gggggagccc ggaattcgct taccggttca ctgagcacgc
     4441 ggtggggaat cacattctca cgcccaactt caagaacggc gtcgggccaa accaatatcg
     4501 atcgggctac gtccgggtag atgccggcgc gagcaacacc aagctgttgt tcccccagat
     4561 gtccaccggg ccactggcaa ttgatcgagt cttgtcggac gagggccgcg ccacgctgtt
     4621 cctgggcgat gatatcagcg acgcgcgggg tctctattta cggcactcgc agggattcaa
     4681 tgtgcttcgg gccaatggga tctccaccac cggtaccccg gcctggaatc tccgcggcag
     4741 tgtggctatc tccggcgggt ctgacaaggc ggatgttcgg ttccagactc ccgaagcgga
     4801 cgccggttac ttcgtgacct gcgccgtaac cggggtcaaa ggctcgccgg cagccgcctc
     4861 ccggcgtgtc tcgatcagcg acaagaatga aaagggattc cgggtgcgag cagaggaggc
     4921 tcccggagga aacaacgcgg ttatggtgga ctggattctg gtccgctagc gagggagccg
     4981 cttcggcccc aggccaacgt caccttgatc cgggcgacga atcgattcgc cgttacgccc
     5041 aactggtttt ggtcgttggt gacgtggtga aaacctgcgt tgccggcgag ctcgaccgga
     5101 ccttcccgtc ccgcaacgcc gagtgcgacc cgataggtct tctcgacggt gccgatcaga
     5161 aacatcggcg tgatctggtt tccattggcg tccgggggag gatatggatc gttgatcttg
     5221 ccttcgcctt gccgttgaaa agtgagctcc ggcgtcagca accattgtcg ggcagcgggg
     5281 accgtcaccg acaccgatag ctgatcgtca tcggagaaat tccggccgat gcccactccc
     5341 tgatcggtga agttttcctg cggattctgc gttcgcagcg ccaggctcga gacaagtgtg
     5401 taccaggccc gccagccgag ccggcttccg ataggaccgt agcccgccac ggtaaacgca
     5461 tacctgtcct gcttctgatt tcgcttgttg aaccaaaagt cgtcgatcgc ggtctgtgtc
     5521 tggagtgtca cgcgccggcc cagccgccag ctcacgtcga agccgatgat cgtgttgctt
     5581 ccggtgtcag cgatgccgaa gttgttcgcc agcacggtgg gagaaagcgg attggcaaac
     5641 ggcgtttcca gtgtgcgccc gacgccctga ataagcacgc tttcccaagg gacgagcagc
     5701 agccgtcggg agacctgtag ctcgaggcgg tgcacgaagt aatagcgatt gaccgactgt
     5761 ccggcggcgt tggcctgagg tctgaggtcg gacgccaggg cacgcagccg gacaattccg
     5821 gtgccgagga gcagctcgag cccctgacgc tcgaatgcca cgtcgctgag gggaatcccg
     5881 ggaacaccaa cgggacccca gttgtgcaac agttgcccat aggtcagcgc gccccatttg
     5941 atctgtccgc tcaggtatcc ctcaatcagc cttccgctga cgttttcctg agccttattc
     6001 ggccaatccg gattcccaat cagactgggt tctatggagg tacgcaccac tccagcgact
     6061 gggccgaaca gcccccagag ctcaaagtcg ccgtacgggt tagccgttcc cgggccaccg
     6121 agatggagcg ggtcacgacg tttttgagta tatgcctcgc cgccgcctcg gagcctgact
     6181 ctccaccaac ttttgtctga tctcacgtcg gagcggaggc tctcattgag gcgatggact
     6241 agacgcccgc tagtggtttc cggtgcggtg tcggcggccg agagcacctt gatcgcgtcg
     6301 gcataacgaa acggccgaat cattggcgac ggatcgacga cgtcgccgcg ggcgatcaga
     6361 tgctcgatca gagggagacg ggagtcgtca agcggcaggt aaggcgacgc ctgagcccgg
     6421 gctgacctga ccccggcgag cagcactagc gcggcgccca cgaacgcgcg cctggtagat
     6481 gacatgggcc ggaaaggtaa cgctgcgctg cttcggggtc agcgggagga gccggttcgc
     6541 cgggctatat tgcgcgctat tgtaccggca ggcgggactt agttggcgac actcggttcc
     6601 attcgaggta ccatgacggc gaaacaggac aacctggatc cgcagacacg ggatctggtc
     6661 cggttcgcgg ctgctattgc ccagggctac gagcccgagc tccgggaacg ggttggcccg
     6721 ctgcggagct cccaggttcc ggtgtcctgg gtcgaggagc tgatcctgca gtcggtgctc
     6781 atgtgcgggt acccccgcgc gctggttgcg ttcatggtct ggcgcaagtt cggtgcggtt
     6841 ccggcgccac aggacgatcc cgaagctcgg tacgacaacg tcgcagcctg gaccgcccgg
     6901 ggagaggaaa catgtgcgct gatctacggc gacaactacc tcaagctccg ggacagcgtt
     6961 cgcgtgctgc atcccgccct ggacggctgg atgatcaccg agggatacgg ccgcaccctc
     7021 gcccggccgg gcctcgacct cactcgccgc gagctttgta ccgtcgccca gaccgcggtg
     7081 ctggaaacgc atcgccagct ccactcccat ctccgcggtg cgctcaatgc cggcgccacc
     7141 ttcgggcaga tcgacggggt gctttcgatt gtgaacccgc tgctttcgtt cgatcaatgg
     7201 aagaagatca aggagctctg gcgcacggtg cgggaggggt ggagtcccgg gcagtgagct
     7261 tcgtcgatcg cgccgcggtc aaagtggttg ccggcacggg cggttcaggt gcctcctcgt
     7321 ttgccaggtt caagtacaag cccaagggcg gtcccgatgg cggagacggg ggtcgcggcg
     7381 gaagtgtcta cgtccgagcc gatcccaacc tgagcacgct gctggactac cgttatcgaa
     7441 ccctttggga ggcggaccgc ggcgagcacg ggaagggcaa gacgcaaacc ggccggtcgg
     7501 gcgaggacat ctatctgcca gtgccccccg ggactgaaat ccgcgacgcg aaaaccggat
     7561 ccccgctggg cgaggtcctg cgaggtgggg acaccgtgct ggtggcgcgg ggaggccgag
     7621 gcgggcgagg caatgcgagg ttcgccaccc cgacccatca ggcacctcgc gagtgggaac
     7681 cgggagaaga ggggcaggaa cgcgagatcg agctgatttt gaagctcatc gctgatgtcg
     7741 gtctggtcgg cgaacccaat gccggcaaga gtacgttgct atcggtcatc tccgccgcgc
     7801 gcccgaagat cgccgattac ccgtttacta cgctcgagcc caatctcggt gtcatggagc
     7861 tgtccgatca ccgttctctg gttgtcgccg acatccccgg catcgtcgaa ggtgcgcacg
     7921 cgggtaaggg cctgggcctc cggttcctcc aacacgtcga acgcacccgg gttctggcgc
     7981 tcctcattcc cctcgatgcc ggcgatcccg gcgccgtcta cgatcggctc cgcaacgaga
     8041 ttcggcgtta cagcggagcc ctatacgaaa agccgcatct gatcgttctc accaagcgcg
     8101 atcttctagc atcggatggt cccatgccac cggttcgcgc cgcggatgga gtggacgtgc
     8161 tcgccatctc gagtgtggct ggcaccagac tcgaggagct caaggagacg ttgtgggcct
     8221 ttgtctcaaa ggcccgtgag gatgagcggc ggcgggaaga cgcatggacc aagagcgagc
     8281 tgcctacgtc gccctggccc tgacgtcagg catcggggcc gcccgaatgg ctaacctcct
     8341 gtcggcctgc gggagtgcac tcggcgcctt ctcggcgccg ttcgcgtttt tacgctcggt
     8401 ccctggaatg aacgcggctg cggcgaccgc gatccgaaac atgtcggtgt ccgacggcga
     8461 acgcgtgctt gccgaggtcc agagcctcgg cggggaaacc attttgccca gtgacgcgtg
     8521 ctatcccgct tcgttccggc ccattgaaga accgccgata gtgctgttcg cagccgggaa
     8581 ccttgggctg ctgggtcggt gcgccgtggc gatcgttggc agccggaacc acacggccta
     8641 cggcgcggct gcgtgccata tggttgcttc ggccttcgcg cgggccggcg gatcggtcgt
     8701 cagcggcatg gcccgcgggc tcgatgccgt cgcccatcag accgccctcg atgcgggcgg
     8761 gggaactatc ggcgtgctgg gcaacgggct cggcgtcgtc tacccagcag ccaaccggag
     8821 gctctacgat gccatggtag accgaggcct tctattgact gagtttccac caggcgagcg
     8881 tccccacgcg ggaagcttcc cccagcgaaa tcgactaatc agcgggttgg cgcgggcaac
     8941 cgtagtggtc gaggccgcac ccggttcggg cgcgctgatc accgccaact gcgctctgga
     9001 acagggcaga gagctgctcg cgcttcccgg accaatcacc agcgccacgt cggtcggcac
     9061 caatcgactc atccgcgatg gtgccacgcc agtggtcgat ccggcggatc tctttcatca
     9121 ttatccggaa ctcgagattg ccggaggctg gacgtcctgg ccggacgccg ctcgctcgag
     9181 cccatcactc cagctcccgc cggacgcggt tgccctggtg gaggcagtcg ggttcgaccc
     9241 ggtccacatc gacaccttgg ccgagcggct gggcctacct cccggcgagt tgctgggctc
     9301 gctttgtacg ctcgagctgg ccggcgttgt ggtacagcaa gccggtggaa tgttccggcg
     9361 agcctgacgg cccggcgata tttcaaagtg cacgtttact tccacatccc cttctgcgcg
     9421 cgccgctgca gctattgcga cttcgcgata gccgtgcggc gtgaggtgcc gtctcgcgcg
     9481 tacgtggagg ctgtcctgcg ggactgggca gctcgattga acgagccgcc ctgggaccgc
     9541 gaggcgatcg tcgaaacgat ctacttcggc ggaggaacgc cgtcgcgtct cgagccgcac
     9601 gccatcgagc agctgttgga tcggattcgg tccgatcgca cggtggccct gggtgcggag
     9661 atcacgctcg aagccaaccc cgatgacgtg agccgcacat cagccgccgc gtggcgtggc
     9721 gcgggagtga accgactatc tctgggagcg caatccttct cggtcgacgc ccttcgctgg
     9781 atgcatcgta cccacgatcc gcacgccata gggcaagcgg tggaggctgc gcgcgcgagc
     9841 ggattcgaca acatctcgct cgatctcatc tttgcgttgc cggacacgat tcagcgaaac
     9901 tgggctcacg acctcgacca ggccctgacg ctcgccccgg agcacgtctc gctctacggt
     9961 ctcaccactg agccgggtac gccgctcgcg cgttggagtg ccagcggccg ggtcagaccc
    10021 actgacgaca cccggtacgc agatgagtac ctgctggcgc acgaacggct cagtactgcc
    10081 ggcttcgagc attacgagat atccaacgcc gggaggccgg gatatcggtc ccgacacaac
    10141 agcgcctact ggactcgggc gccctatttg ggcgtcgggc cgtcggcgca tagcggacac
    10201 ggtaatcgac gggagtggaa tattcgcgag tggaccgcgt acgaacgcac cgcccgcgca
    10261 ggcgcggagc tgatcgaggg gcgcgaggct ctcgaccaag ccgcggttcg actggaggag
    10321 atctatctcg ggctgcgcac gctcgagggg gttccccggg actggatccc cgaatcgaaa
    10381 tccgcggaat ggagacggcg ggattgggcg ttggtgtccg atggccgcct gaggctgacg
    10441 gtggagggat ggctcagact ggacgcgctg gttgcctcgc tttgatcggg cccgtttcga
    10501 gtcctcgcgc ccgggtctag atttgttgga tgtccgccca agaccaactg tccgagcgcg
    10561 agctgcgcgt gctcgaggct gtggtgcaaa cctacatcga gaccgcggaa ccggcgggca
    10621 gtcagaccat tgcccgccga ttcggcttgg gcgtgtcgcc ggcaaccatt cgcaacacca
    10681 tgagcgagct ggaggagaag ggttacctct ttcatcctca cacctccgcc ggccgcgtcc
    10741 ctactgaccg ggcctaccga ctgtacgtgg acggcctgat ccggctcgcg ccgccgtcac
    10801 tggaagaatc caaagcgctg cgagcggagg ttctcgggtc gggaagcacc ctggaagaga
    10861 ttcttcggcg ggcggctcag gtgctcgggg tacttaccca ggagttgggc gtcgccgtcg
    10921 cgccggctct cgacgagctg gtattagagc ggctcgatct gataccggtc acatcggacc
    10981 gggtggtcct ggttttcaat ctccaaagcg gggtagtacg gaccatcttc gtgcaggtcc
    11041 ccagcaacct gccgggtcca tcggttcaac gagtcaccca gatcctgaac gaccgtctgt
    11101 ccggtctcac cctggcccaa atacgccaga gcctcgtgga gcggctgcgc gacgcagatc
    11161 acgcgcccga cagtcgcgag ctgctgaaca ttttcatcgc cgagcgggac gacatcttcg
    11221 atctgtcggg caacggcgcg accgtcatgc tcggcagcgc gcaaatgctg gcggaccagc
    11281 cggagttcgc gtcgaacctc ggtatgcgcg gcctgctgga gctgaccgag cggcgtgacc
    11341 tcctgcgtcg ggccctcgag gcgcgacgcc gggcggggct ctcgatcacc atcggatggg
    11401 agaacctcga ccctcgactc agcaacttta ccctggtgac atcatcgtat caggcgggtg
    11461 aactcaaggg tgtgatcggg gttatgggcc cgactcggat gccgtacgag aagatcatag
    11521 gcctggtgga acacaccagt cgcctggtgg aggacctgct ggagtgaacg aattttacga
    11581 actgctcggg gtatcgcgga ccgcgtccga ggccgacatc aagaaagcct atcggaagct
    11641 cgcgatggac taccatcccg atcgcaacaa gtcggccgaa gccgaagcca agttcaagga
    11701 gatcgcggag gcctacgagg tcctgcgcga cccgcaaaag cgcgccgcgt acgatcgcta
    11761 cggacgggcc ggcgtgagcg gcgccggtgc cggagggttc ggcttccatc atgtggatct
    11821 gtccgaagcg ctcgagatct tcatgcgcga cttcggcgga ctgggcggac tcgagtcgat
    11881 cttcggaggc ggacgggctc aggccgagtc gcggcggggc caggatatca gagtcaccgt
    11941 caggctcacc ctggcggagg tcgcgacagg caccaagaag agcgtcaagc tgaagacgct
    12001 ggagcggtgt gagaactgtg cgggcagcgg cgcgcggccc ggcaccaagc cgtccgtctg
    12061 ctccacgtgt ggtggcagcg gggaagtgcg ccgcacggcc cgcagcatgt tcggacagtt
    12121 catgtcggtg tcgccctgtc ccacctgcgg cggcgacggc atggtcgtac tcgagccctg
    12181 cgaggtgtgc cggggcgatg ggcgggtgcg gggcgagcgg accgttccgg tagaaatccc
    12241 ggcaggtgtg tcggccaaca attacctgac ccttcggggg cagggagccg ccggcccgcg
    12301 caacggtccg aacggcgacc tgctggtcat gctcgatatc aaggaggacg agcggttcga
    12361 gcggcagggc aacgacctcc tgttcgatct tccgctttcg ttctcacagg ctgcgcttgg
    12421 tggagagttt caggtgccca cgccgcacgg cgaggagcgg gttcgagtgc ccgccggcac
    12481 tcaaaccaac actgtgctca gactcaaagg gaaagggctg ccggcgctgg gccagagtgg
    12541 aagtggtgac ttcatgattc gcatccatgt ctggacgccc gagcgactct cggacgagca
    12601 ggagcggctc tttcgagagc tggccaagca cgaaggcgag ccgccccggc gcgctccagg
    12661 attctggacc aagctcaaag aagccctcgg cgcatgaagt ggtgggctgt cgacgtgctg
    12721 atctctcccg aacgccggga gtcggtcggc gcgtggctcg tggcgcgcac gggccatgcg
    12781 gtcgaagagc gagccgacgg cacgttggta ggcttcgccg aggatgaggc cgcggcggag
    12841 cggctctccg tcgcggtcgc agacgaggcg ggggtgccgg tacaggtctc gtgccgaccc
    12901 ctcgaaacgg tggactggag ggatcgctgg cgggacggct tgagtcccag gcggattggc
    12961 cggctcacag tcacgccctc gtgggccgct ctagatggtc tgccgtccga accggttct
//
LOCUS       DASRJQ010000026         2476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3369653, whole genome shotgun sequence.
ACCESSION   DASRJQ010000026 DASRJQ010000000
VERSION     DASRJQ010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3369653"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            367..1155
                     /locus_tag="VFB89_00655"
     CDS             367..1155
                     /locus_tag="VFB89_00655"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZM25848.1"
                     /translation="MPRVERFGVSISYEVAGHGFPLVLLHGLSVDGASWRRAGYVDAL
                     SHRYRCISIDSRGAGGSDKPDLPALHTMDHSLGDVEGVLEREGIDAFAVWGFSLGGGI
                     ALALAARHGKRVRAVVSTGAWDTRPYTLEDIRKTRSIMDPSEGGGMAAMLATWEPDED
                     PALPEWFRKTILDYDHRAWLAARYGAFCWPDVPVAAITPPALIIVGSREDPAGDALRW
                     AATLPDGRCAIIPERTHCGAFLATEQSLAHAGPFLDSVVGSRAR"
     gene            complement(1616..2212)
                     /locus_tag="VFB89_00660"
     CDS             complement(1616..2212)
                     /locus_tag="VFB89_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25849.1"
                     /translation="MIDKETWNERSIAAGLLLLGFLLVFPAVFINAPGMGANSKWTQS
                     FPFLFDSVWQKYWSIAFVVITLYGLVIFESVLRQAGDHIFSRLGAVSFTLASAMWLVV
                     IVLDLHDLPGGRDFESFFIVLAFPAVLAYGLGILRTKVLARWIGITVVLWSIVMLVRV
                     FPHNEGPLFYEPALVLIAVALLLTRNQQHAPTESQADS"
BASE COUNT          445 a          751 c          813 g          467 t
ORIGIN      
        1 tatcggtacc gtcgtgcccg gtccctcgta ctccagccgt tgatcatgat ctagcagggg
       61 ccacaggcat tgccagctga tcgagtaagc cttgaaacta gggcctcttc gtcccgaacc
      121 tggcgcagct gggtcatccg cggttcacca cgcggatggc tggtcatcga cggtcgccca
      181 cggccggctt gaaacgcctg tgctgctgta cttcagtgct gtactgagtc gctgcctgag
      241 ccgcgatctg ccggcgactc aacagtgctg atcgggtatt cggagccgcc ggcgttggaa
      301 gcaaccttgt gcgattgaac ccgggccgct caacattgct ggtggccgtg gtggaagggg
      361 ggcctgatgc cgagggttga gcggttcggt gtgagcatct cctacgaggt cgccggccac
      421 gggtttccgc ttgtgttgct gcacggactg tccgtggatg gcgcgtcctg gcgccgggcc
      481 ggttacgtgg atgcgctgag ccaccggtat cgatgcatca gcatcgactc ccgtggagcg
      541 ggcgggagcg acaagcccga ccttccggcg cttcacacga tggatcactc cctcggtgac
      601 gtcgagggtg tgctcgagcg ggagggtatc gacgccttcg cagtgtgggg attctcactc
      661 ggtggtggga tcgcgctggc gctcgcggcc aggcacggca agcgggtgcg tgccgtggtc
      721 agcacggggg cgtgggacac ccgaccgtac acgctcgagg acatccgtaa aacgcgatcc
      781 atcatggatc catccgaggg cggcgggatg gccgcgatgc tcgcgacctg ggagcccgat
      841 gaggatcctg ccctgcccga atggttccga aagacgatcc ttgactacga ccatcgcgcg
      901 tggctggccg ccaggtacgg cgcattctgc tggcccgatg ttccggtcgc cgccatcacc
      961 ccgcctgcgc tgatcatcgt tggctcccga gaggaccctg cgggggatgc gctgcgctgg
     1021 gctgcgacgc ttcccgacgg tcgatgcgcg atcatcccag agcgcaccca ttgcggcgca
     1081 ttcctggcta ccgagcaaag cctggcgcac gccgggccgt tcctggacag cgtggtgggc
     1141 tcccgtgcca ggtaagaggc tcacaccggt gggggggctg cgagcctagg ggtcacgtcc
     1201 caccgcctcg acttcagttg aagccgcctg agcggatctt gccctcgacc tacgcattac
     1261 gagtgggttc ttgtgtgcga gatgaaacac ccgcctccca ccttgctgca tggatgccgt
     1321 cgctgagagc ggattgttgt tggcagttgc cggccgctcg gggacatctc ggggacaccg
     1381 tcgtgccctt tggcgcctcg agctgaacca acgtatggga cctcgacctc ttcgtcccga
     1441 actcggcgca gctgcgtcat ccgaggttca ctacgcgggt ggctggtcat cgacggtcgc
     1501 ctacggctgg cttgaggtgc cggtgctgct gtacttcagt gcgtaccgag ctcgtcgccc
     1561 tgcggccggg tcggcgtctc aaagcgccgg cgggcctccc ggaccggctc agtgatcagc
     1621 tgtcggcctg gctttcggtt ggggcgtgtt gctgatttcg tgtcagcaat agagctaccg
     1681 cgatcagcac cagagcaggc tcgtaaaaga gcggaccttc attatgtggg aagactcgta
     1741 cgagcatgac gattgaccag agtacaacgg tgatcccgat ccagcgggct agcactttgg
     1801 tgcgcaagat ccccagcccg tacgcgagta cggcgggaaa cgcgaggacg atgaaaaagc
     1861 tctcgaaatc gcgcccaccg ggcaaatcat ggaggtcgag cacgatgact accagccaca
     1921 ttgctgaagc gagggtgaag ctcactgcgc cgagcctaga gaagatgtgg tcaccggcct
     1981 gacgtaggac gctttcaaag atcactaacc catagagggt gataacgacg aaagcaatgc
     2041 tccaatactt ctgccagacg gagtcgaaga gaaaaggaaa actctgagtc cacttcgaat
     2101 tggctcccat acctggcgca ttaatgaata cagctggaaa aacgagcaga aagccgagga
     2161 ggagcaagcc tgccgcgata ctacgctcgt tccacgtctc cttgtcgatc actggactct
     2221 cctgcgacag ttatcggatc tggaccggat tcaaacgaga taccactacg acgacgcgcc
     2281 aggttttgga cgcacggcga cctctgggtc gtgatcgtag agggtggttc gccggcgagc
     2341 acggcgcgcc gccggccggg gcacggattg ggcacgctcg gagggtcgcg atcgggtgcc
     2401 agcggcacgt gtcggtggcc ctcggcgacc accgctttgc aaagaggatc ccgaccatcc
     2461 gctggtgcct cagtgc
//
LOCUS       DASRJQ010000027         4151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1391759, whole genome shotgun sequence.
ACCESSION   DASRJQ010000027 DASRJQ010000000
VERSION     DASRJQ010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1391759"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /locus_tag="VFB89_00665"
     CDS             complement(<1..1135)
                     /locus_tag="VFB89_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25850.1"
                     /translation="MGFGCMVLFALPFAAVGLVSGVMVVSKLAAGDWRTAAFLGLFAV
                     LFGGAGTGLIAAAWAGRQETARRQELQTRHPDEPWLWRQDWAAGRIDDGGRKGQYALW
                     AFAALWNLIAFPSAFFALREFQRSGNRLSLIALVFPLVGIGLIVAALRSSDRQRKFGL
                     STFELATLPAVIGHGIAGTVRIASELLPPEGFLATLSCVNLRTTGSGDDRSTTETIRW
                     QEQQKMVGQRASGGPGSGLVTSVPVHFRLPPDVSPTDDANYNDRIVWRLELTAAVPGV
                     DYDARFEVPVFRTAASVEPLTPEQEKLLGPPAESLPYKQPPNSPIRVSQSARGTQIVF
                     PPARNPATALGVTGFAALWGGAVWLILHFKAPVFFAIVFGAVEL"
     gene            complement(1192..2067)
                     /gene="sseA"
                     /locus_tag="VFB89_00670"
     CDS             complement(1192..2067)
                     /gene="sseA"
                     /locus_tag="VFB89_00670"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF008557.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HZM25851.1"
                     /translation="MPADKLAPLVSTEWLAAHLGHPDVRVVDGSWYLPTMNRDAAAEF
                     VEGHIPGAVFFDLDASSDQSTALPHMLPPASAFATRMAALGLDDANTIVIYDGSGVNL
                     SAPRVWWMLRVFGHEAAAVLDGGLGKWKREGRPLERGSVSPAGGRFTARLDRSRIRDF
                     EAVRANLSTGVEQMVDARPAGRFEGTQPEFRPGVRSGHIPGSLNLPFNELTTDGGTLL
                     PPKQLRRRLVSAGIDLARPVVVTCGSATSACNLVLGLEVLGHNDVAVYDGSWTEWGGR
                     EDTPIELGPPRAAHS"
     gene            2150..3787
                     /locus_tag="VFB89_00675"
     CDS             2150..3787
                     /locus_tag="VFB89_00675"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZM25852.1"
                     /translation="MAIASLGSEPAVEPSTSSRTVLRILQFGVVAVVLAALPYPLFDL
                     DRHGVPKELVASLTGFGAATYALVHTRKLEITAADALLALFLAVSLVSAVFAENHWLA
                     FRAFGLSLSGALVFWSARSVARSGHAEPLVRTLAAAVVLAAFTALLQAYGVELAIMAE
                     RRAPGGTFGNRNFMAHFVALGAPVLALAGLEARRRWEGFLWQAGVAVAGAALVLSRSR
                     AAWLAFTLGLASFAVEGLWAGGLWKNQVARSRVIALALAAGIAAGLALVLPNHLEWRS
                     NSPYLDSMRAMTDFREGSGRGRLVQYRNTLRMATDHPAIGVGPANWAVDYPKYTFPGD
                     PAFDPDDFIPTNPWPSSDWVALLAERGPLALVVVLVLGAALAVGAWRRWRQNSETTQG
                     LEALVALLTLLVLACVSAFDAVLLLPAPTVFVGALLGALIRPPRRSLFTLLLSDLRRA
                     RLAGAVALVGTLLTVQSLGQIGAMAVFTGSGKPATREWAARLDPGSYRIHMLLAYEWR
                     NRGRCNRARPHAEAARMLYPNHPAPRQLLAACGVKSR"
     gene            complement(3885..>4151)
                     /locus_tag="VFB89_00680"
     CDS             complement(3885..>4151)
                     /locus_tag="VFB89_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25853.1"
                     /translation="ALNQPLPAGLWSLNGTSTWIRNSGTANEKSWTFTTTAPSVHFDP
                     TCTSAPQFDSGTMTVSATNGQNSNTAQFTITFTGCGKYEVVITH"
BASE COUNT          707 a         1355 c         1354 g          735 t
ORIGIN      
        1 cgagctcaac ggccccgaac acgatcgcaa agaagacagg cgccttgaaa tgaagaatca
       61 gccacacggc tccaccccac agcgcggcga agccggtaac cccaagtgcg gtggccggat
      121 tccgggctgg agggaaaacg atctgggtgc cacgagcgct ctggctcacc cgaatcggcg
      181 agttgggcgg ctgtttatag gggagcgact cggcgggcgg accgagcagc ttttcctgct
      241 cgggggtgag aggctcgaca ctcgctgcgg tgcggaatac cggaacctcg aacctcgcgt
      301 cgtagtcgac acccggcaca gcagccgtca actcgagccg ccagactatc cggtcattgt
      361 aattggcgtc atcggtggga gagacatcag gcgggaggcg gaaatgcacg ggtacggagg
      421 tcacgagtcc gcttccgggc ccaccgctgg ctcgctgccc taccatcttc tgctgttcct
      481 gccaacgaat ggtctcggtc gtcgaccgat catcaccgga cccggtggta cgaaggttta
      541 cgcacgagag cgtcgcgaga aagccctcag gaggcagcag ctccgaagcg atccgcactg
      601 tccccgcgat gccgtggccg atcacggcgg gcagcgtcgc aagctcgaag gtcgagagcc
      661 cgaactttcg ctggcgatcg ctgctgcgca gcgccgctac gatgagcccg atgccgacca
      721 gtggaaagac gagcgcgatc agcgaaagtc ggtttccaga tcgctgaaac tctcgcaggg
      781 caaagaacgc cgaggggaag gcaatcaggt tccagagtgc cgcaaacgcc caaagcgcgt
      841 attggccctt ccgaccgcca tcatcgatcc tgcccgccgc ccaatcctgt cgccagagcc
      901 agggctcgtc cggatgcctg gtctgtaatt cctgtcgtcg ggccgtttcc tgacggccgg
      961 cccacgctgc ggcaatgagc cccgttcccg cgccgccgaa caggacggcg aaaaggccga
     1021 ggaaggcggc tgtgcgccag tctcccgcag caagcttcga caccaccatg acacccgaga
     1081 ccaggccgac ggcggcgaac ggaagcgcaa agaggaccat gcaaccgaag cccatggccg
     1141 gggacttgag ccacgccggc gttgccgatg atccgggcgt aaggcgggtc ctcacgaatg
     1201 ggcggcccga ggcggtccga gctcgatggg cgtgtcctcc cgcccacccc actcggtcca
     1261 gctgccatcg taaacagcga cgtcattgtg tcccaggact tcgaggccca gcacgaggtt
     1321 gcacgcgctg gttgccgagc cgcaggtaac gaccacagga cgcgcgaggt cgattcccgc
     1381 ggatacgagt cgacggcgga gttgtttcgg tggaaggaga gtgccgccat cggtggtcag
     1441 ctcgttgaac ggcaggttca ggctgccggg gatgtgacca ctcctgacgc ccggccgaaa
     1501 ctctggctga gttccttcga aacggccggc cggccgagcg tccaccatct gttcgactcc
     1561 ggtcgacagg ttcgcccgta ctgcctcgaa atcgcggatt cggctccggt cgaggcgggc
     1621 cgtgaagcgg ccgcccgccg ggcttaccga tccgcgttcc agtgggcgcc cttccctctt
     1681 ccacttccca aggccgccgt ccagcacggc cgcggcttca tgcccgaaga cccggagcat
     1741 ccaccagacg cgcggagcgc ttaaattcac gcccgaccca tcgtagatca cgatcgtatt
     1801 cgcgtcgtcg aggcccaggg ctgccatgcg cgtggcgaag gccgatgccg gcggaagcat
     1861 atgcggcagc gcagttgact ggtcgctcga cgcgtcgaga tcgaagaaaa ccgcgccggg
     1921 aatgtgtccc tccacaaatt cggcggcggc atcgcggttc atggtcggga gataccagga
     1981 gccgtccact acgcgcacat caggatggcc caggtgggcg gcaagccact cggtggacac
     2041 cagtggcgcg agcttgtcgg cgggcatgaa caatcttagc gcaagaccca gtgtccgatg
     2101 gaggccgtat ccagcctggt ttcgtgaacc acactaacgg ctcaggctga tggcgatcgc
     2161 gtcgcttggc agcgagccgg cggtcgagcc cagcaccagc agccgaacgg tccttcgcat
     2221 cctccagttc ggcgtcgtcg ctgtcgtgct cgccgcgctc ccctatcctt tgttcgacct
     2281 ggaccggcac ggcgtgccca aggagctggt ggccagcctc accggtttcg gcgctgcgac
     2341 gtacgcgctg gtacacaccc ggaagctgga gatcacagcc gctgacgcgc tgctggcgct
     2401 cttcctggcg gtttcgttgg tctccgctgt gttcgcagag aatcactggc tcgcgttccg
     2461 ggccttcgga ctgagcctgt ccggagcgtt ggtgttctgg agtgctcgaa gcgtagcacg
     2521 atcaggtcac gccgaaccgc tggtgcggac gctcgctgcc gcggtggtcc tggccgcgtt
     2581 caccgccctt ctccaagcct acggagtaga gctcgcaatc atggcggagc gtcgggcacc
     2641 gggtggaacg ttcggcaacc gcaacttcat ggcccacttc gttgcgctcg gcgcaccggt
     2701 gctggcattg gcgggactcg aggctcgccg tcgctgggaa ggctttctgt ggcaagctgg
     2761 ggtagcggta gccggtgcgg cacttgtgct gtcgcgctcg agagcggcct ggctcgcctt
     2821 tacgctcggg ctcgcgtcgt tcgcggtgga ggggctctgg gccggcgggc tctggaagaa
     2881 ccaggtcgca cgcagtcgag tgatcgcact tgctctggca gccggcattg cggccgggct
     2941 cgctctcgtt ctcccgaacc acctcgagtg gcggtcgaac tctccttacc tcgattcgat
     3001 gcgggcaatg accgacttcc gcgaaggcag cggtcgcggc cggttggtcc agtatcggaa
     3061 tacgctgcgg atggcgaccg atcatccggc gatcggcgtc gggcccgcca actgggcggt
     3121 cgactatccg aagtacacat tccccggcga ccccgcgttc gatcccgacg acttcatccc
     3181 caccaatccg tggcccagca gcgactgggt cgcgcttctc gccgagcggg ggccgctggc
     3241 gctcgtggtg gtgctggttc tgggtgctgc actggcggtg ggagcctggc gtcgctggcg
     3301 ccagaactcc gagacgacgc aaggcctcga ggctctggtt gccttgctca cgcttctcgt
     3361 gctcgcctgc gtcagcgcct ttgacgccgt cctgctgctt cccgccccga cggtgttcgt
     3421 cggcgccctg ctcggcgcct tgatcaggcc accgcggcgc tcactgttca cgctgctgct
     3481 gtcggacctt cggcgagccc ggctcgccgg tgcagtcgct ctggttggaa cgctgctgac
     3541 ggttcagtca cttggtcaga tcggcgccat ggcggtgttc acgggaagcg ggaagcctgc
     3601 aacgcgggaa tgggccgccc gactcgaccc cggcagttac cggattcata tgctgctcgc
     3661 gtacgaatgg cgcaatcgcg gccgctgcaa ccgggcccgg ccgcatgccg aagctgcgcg
     3721 gatgttatat ccgaaccatc cagccccgcg gcagctgcta gcggcgtgtg gggtgaaaag
     3781 ccgttagcca gtaaaagcga gaaaaggaaa aagggaacgg ggaagcggcg gccacccttt
     3841 ctcgctttct cctcttcacc cttgcactgg cttacagccc atgtctagtg ggtaatcacc
     3901 acctcatact tcccgcagcc cgtaaacgtg atcgtgaact gcgccgtatt gctgttctga
     3961 ccgttggttg cgctgaccgt catcgttccc gagtcgaatt gcggcgccga ggtacacgtc
     4021 gggtcgaagt ggacgctggg cgccgtggtg gtgaacgtcc agctcttctc gttggcggtt
     4081 cccgaattcc ggatccaggt cgaagttccg ttcaatgacc ataaccccgc gggcaacggt
     4141 tgattcaagg c
//
LOCUS       DASRJQ010000028         2153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_4201407, whole genome shotgun sequence.
ACCESSION   DASRJQ010000028 DASRJQ010000000
VERSION     DASRJQ010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4201407"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2153
                     /locus_tag="VFB89_00685"
     CDS             <1..>2153
                     /locus_tag="VFB89_00685"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HZM25854.1"
                     /translation="PRFQPGNDKSWSPHRLDRPGAINALFAEVLAETGRRLNRAAPAI
                     DQLIVAGHSRAFGILYPLARASASPELGKGALARLSRIWALDATYGKPPIAAFESLTK
                     AKQGLGVEIVYRAKSATDKFKGASRAGALALRPIPRSITHCAVPGWMLPALLAELPPP
                     RPEALDAGSEAEDLWAETPGSGWSESGVTETEWLEAEADALDRFDTLDSQTWDESHTF
                     GESGAFEEASLESEDESPDRFDELGDGRNSEWESDEAEPEEEALDEESVESQELFEAE
                     EFARHALYEVGLDEDEIELGSAAEESPAEAPSSRPRNLTSSQVRKAWRAYAGAKHRMV
                     PLRLFGRWNPPVNPLTVDAWRALERALGASNYDVHRAWVFVGRSIAGTQTHSLHAYGL
                     AIDIDHAKPTCNVNRATPDHRKVRFSSAATKEGRCQDVRDRKADTSFTRDQVAAVEAI
                     RTVDGHQVFVWGGRWQNIKDTMHFEINVTPDELARGLQPDPGHRLQLADPAGEFEEEY
                     LEVDASMSDVSLGTLTFSAPGWKAFTYRFTAEDLVWAARFMAGEAGGVDNLENRAVLW
                     LMFNRYAYFRHEHPSWGTFGDFIRRYSTPLQPYLYSLGAAKRHWRKCNSSYTNCDYVP
                     MAERYGYYKSTQIPRGQLRQFIKLQQRPWSKLPASARDLALRALQGAIPNLIGTASEF
                     DDTATYYKDDNGERPTRQQWLDYTKDFAAKKQYLW"
BASE COUNT          412 a          715 c          696 g          330 t
ORIGIN      
        1 tgccgcgctt ccagcccggc aacgacaaga gctggagccc gcaccggctc gatcgtcccg
       61 gcgcgatcaa cgccctgttc gccgaagtgc tggcggaaac cggccgccgc ctgaaccgcg
      121 ccgcgcccgc gatcgaccag ctgatcgtcg ccggtcattc gcgggccttc ggaatcctct
      181 atccgctcgc ccgcgcttct gcttccccgg agctggggaa gggggcgctg gcccggctgt
      241 cgcggatctg ggctctcgac gccacctacg gcaaaccgcc catcgccgcc ttcgaaagcc
      301 tgacgaaggc gaaacaaggg ctcggggtcg aaatcgtcta ccgcgcgaaa tcggccaccg
      361 acaaattcaa aggcgcttcg cgggccggcg ccctggccct gcggccaatc ccccggtcga
      421 tcacgcattg cgcggttccg ggatggatgc tgccggcctt gcttgcggag ctgccgccgc
      481 cgcggccgga ggctctggac gccggctctg aagcggagga tttgtgggct gagacgccgg
      541 ggtcggggtg gagcgagtcg ggggtgaccg agacggaatg gctggaagcg gaagccgatg
      601 cactcgaccg gttcgacaca ttggacagcc agacctggga tgaaagccat accttcggcg
      661 aaagcggggc gtttgaggag gcgtcgctcg aaagcgagga cgagtcaccc gatcgtttcg
      721 atgagcttgg cgacggacgc aattccgaat gggaatcgga tgaagccgaa ccggaggagg
      781 aagcccttga cgaggagtcg gttgagtccc aggagctgtt cgaggctgaa gagttcgctc
      841 gccatgccct ctacgaggtc ggcctcgacg aggatgagat cgagctggga tccgccgccg
      901 aggaatcgcc agccgaggca cccagctcgc gcccacgcaa tctgacctcc tcacaggtgc
      961 gaaaggcctg gcgcgcgtac gcgggagcca agcaccgaat ggtgcccctt cggctgttcg
     1021 gaaggtggaa ccccccggtc aacccactca ccgttgacgc gtggcgcgca ttggagcggg
     1081 ctctgggcgc cagcaactat gacgtccata gggcctgggt tttcgtgggc cggtctatcg
     1141 ccggcactca gacccactcg ttgcacgcgt acggtctcgc aatcgacatc gaccacgcga
     1201 agcccacgtg caacgtgaat cgggccacgc cggaccaccg gaaggtccgg ttctcatcgg
     1261 ccgccaccaa ggaagggcgg tgccaggacg tccgcgatcg gaaggccgac accagcttca
     1321 cgcgcgacca ggtcgcggcc gtcgaagcca tccgaacggt cgacggccat caggtgttcg
     1381 tctggggtgg ccgttggcaa aacatcaaag acaccatgca cttcgagatc aacgtcacac
     1441 ccgacgagct ggcccggggc ctgcaaccgg acccgggcca ccgtcttcag ttggccgacc
     1501 cagccgggga gttcgaggag gagtacctcg aggtcgatgc gagcatgtca gacgtgtccc
     1561 tcgggacact cacgttcagc gcccctgggt ggaaggcgtt cacctaccgc ttcacggccg
     1621 aggacctggt gtgggcggca cggttcatgg ctggcgaggc aggcggggtc gacaacctcg
     1681 agaaccgggc ggtgctctgg ctgatgttca accgctacgc ctacttccgc cacgagcatc
     1741 catcctgggg caccttcggg gacttcatcc gcaggtactc caccccgctg cagccgtatc
     1801 tgtacagcct gggagctgcc aagcggcact ggcgcaagtg caacagctcg tacaccaact
     1861 gcgactacgt gcctatggcg gaaaggtacg gctactacaa aagcacgcag atcccaagag
     1921 gccagcttcg ccagttcatc aagctccagc agcgcccctg gagcaaactg cccgcctccg
     1981 cccgcgactt ggcgttgcgg gcactccagg gcgcgatccc gaatctcatc ggcactgcca
     2041 gcgagttcga cgacacagcg acctactaca aggacgacaa cggcgagcgc cccacccgtc
     2101 agcaatggct ggactacacc aaggacttcg ccgccaagaa gcagtatctc tgg
//
LOCUS       DASRJQ010000029         4600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1953028, whole genome shotgun sequence.
ACCESSION   DASRJQ010000029 DASRJQ010000000
VERSION     DASRJQ010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1953028"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            318..1637
                     /locus_tag="VFB89_00690"
     CDS             318..1637
                     /locus_tag="VFB89_00690"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZM25855.1"
                     /translation="MRLSICLLVVIIVVGLLAHRVIALTDKVFIPVLVYRTGPYSVSG
                     TPIANGLVDYYTLVNERDGGINGVRLTWEECETQYNTLRSVECYERLKGKGAVVVFPI
                     STGVMYQLIPRTAEDKISVIQVGAGMTASADGRWFPWVFPFPTTYWNQASAVIRYIGQ
                     REGGLEKLRGKKIVHIFLNVPYGREANPILETLARQFGYQLTLLPVNPPGETQQATWL
                     QIRRLNPDWIFLSGWGVMNQVAIKEAVAAGYPMDHLIGNWWSASEGYLIGVGPAAKGY
                     IGAAFHAPGADFPVHRDILKFVYDRGKGAGKREAIGEVLYNRGVVSAMYEVEAIRSAM
                     SRYGNQPITGEQMRWGLEHLSLTEQRIDELGMRGFIHPLTVTCEDHEGGGPVMFQQWD
                     GSRWTAISGWIPVMRDVVRAKLEAVAAEDGRKLGNTMRDCAKEERSR"
     gene            1634..3670
                     /locus_tag="VFB89_00695"
     CDS             1634..3670
                     /locus_tag="VFB89_00695"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZM25856.1"
                     /translation="MTGVSWSLPRLVARVPATVHAKLLVAFLAIVVLLVMLGAVGLEL
                     LGRLHRRAEDLVKREERIAAYRQFQRDTLLPPSWSWNQNTVQAALRQLNQFGYELDQL
                     QVAAADDAELLGRVRATYDQFIAVVERVAEQWKAGNVAQGRKLQLTEASPLGDRLERF
                     TNDLVNKAEIDLAASIDASQAAYRASRWVVIGFAVGTTALALVLGYAISWSLIGPVKL
                     METRLKLIAAGDLAQRVDVPNRDELGGLAAQFNQMAIELEVRTRELTRLVGELRALGE
                     VSQAVSSSLELETVLETIVARAAQLSAAHAGVIYEYDSATQELHVQSAYRLDDDLAEI
                     MRVAPLRLSEGVAGRAVALRAPFQVADVLDKHAYDVGRIRTAFERHGYRSVVAVPLLF
                     EQQILGALVVWGKETGSFPPEVVDLLQTFANQSVLAIQNARLYREIGAKSRQLGVASQ
                     HKSEFLANMSHELRTPLNAVIGFSEVLIDRMFGELNEKQEEYLKDIYASGTHLLSLIN
                     DILDLSKIEAGRMELELTDFHLPTAIDNALMLVRERAGRRSIALHTSIDHRFGHIQAD
                     ERKVRQVVLNLLSNAIKFTPEGGRIEVGAQPKDRFIEVSVSDTGVGIAPEDQEAVFEE
                     FRQVGVAAKKVEGTGLGLTLCRKFVELHGGRIWVTSEIGVGSTFTFTIPVRLGE"
     gene            3985..4566
                     /locus_tag="VFB89_00700"
                     /pseudo
     CDS             3985..4566
                     /locus_tag="VFB89_00700"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT          841 a         1439 c         1505 g          815 t
ORIGIN      
        1 cggcgactcg gcggtgatca ggtggattcg catccagagc tgtggacata gtcgcccaag
       61 atggcggcgg cggccgctgg tttaaatccg gcgcccgcag ccaatgaaca cgcggggtta
      121 gctgacgtcg aagccgctga cccccttagt ctacccagcc ccctcccggc cgcagttgac
      181 taccggccac tcgaccatct aatctcccta tggttcccta gtatttccca tggttctgac
      241 ctagaccggc ccggactccg gagggcggta tgcagtgtcc cgggttgcca gcacgacggc
      301 acgcggtttg gtacgtgatg cggctcagca tctgccttct ggtcgtaatc atcgtggtgg
      361 ggctactcgc acaccgcgtg atcgccctga cggacaaggt gttcatcccg gtgctggtgt
      421 accggacagg cccctactcc gtgagcggca cgccaatcgc caatgggctc gtcgactact
      481 acacgctcgt caacgagcgc gatggcggga tcaacggcgt ccggctcacc tgggaggagt
      541 gcgagacgca gtacaacacg ctgcggagcg tggagtgcta cgagcggctg aaggggaaag
      601 gggccgtggt ggtcttcccc atcagcaccg gtgtgatgta ccagctcatc ccgagaactg
      661 cggaggacaa gatctcggtc atccaggtgg gcgccggcat gacggcctcg gccgacggcc
      721 ggtggttccc ctgggtgttc cccttcccca cgacctactg gaaccaggcc tcggccgtga
      781 tccgctacat cggtcagcgg gaaggcgggc tggagaagct ccggggtaag aagatcgtcc
      841 acatcttcct caacgtcccc tacggcaggg aagccaaccc gatcctcgag accctcgccc
      901 gccagttcgg ctaccagctg acgctgctgc cggtgaaccc tccgggcgag acgcaacagg
      961 ccacctggct ccagatccga cgactcaacc ccgactggat cttcctgtcg ggatggggcg
     1021 tgatgaacca ggtggccatc aaggaggccg tggccgccgg ctatccgatg gatcatctga
     1081 tcggcaactg gtggtcggcg agcgagggct acctgatcgg cgtgggccct gccgccaagg
     1141 gctacatcgg cgcggcattc cacgccccgg gcgccgactt cccggtgcac cgggacatct
     1201 tgaaattcgt gtacgaccgg gggaagggcg ccggcaagcg cgaggccatc ggcgaggtgc
     1261 tctacaaccg tggcgtggtc agcgccatgt acgaggtcga ggcgatccgg agcgccatgt
     1321 cgaggtacgg caaccagccc atcaccggcg agcagatgcg ctgggggctc gagcacctga
     1381 gcctcaccga gcagcggatc gacgagctcg gcatgcgggg cttcatccat ccgctcaccg
     1441 tgacctgcga ggaccacgag ggcggcggcc ccgtcatgtt ccagcagtgg gatggcagcc
     1501 ggtggaccgc gatctccggc tggatcccgg tcatgcgtga cgtcgtccgc gccaagctcg
     1561 aggcggtcgc ggccgaggac ggccggaagc tcgggaacac gatgcgcgat tgtgcgaagg
     1621 aggagcgatc ccggtgaccg gggtctcatg gtcgctgccc cggctcgtcg cgcgagtgcc
     1681 ggccacggtg cacgccaagc ttctcgtcgc cttcctcgcc atcgtggtcc ttctggtcat
     1741 gctcggcgcc gtcggcctcg agttgctcgg ccggctccac cggcgcgccg aggatctggt
     1801 gaagcgggag gagcggatcg ccgcctaccg tcagttccag cgcgacaccc tgctaccgcc
     1861 ttcctggtcc tggaaccaga acacggtcca ggcagccctg cggcagctca accagttcgg
     1921 gtacgagctc gatcagcttc aggtcgcggc ggcggacgac gcggagcttc tcggccgcgt
     1981 ccgcgcgacc tatgaccagt tcatcgcggt cgtcgagcga gtcgccgagc agtggaaagc
     2041 gggaaacgtc gcccagggca ggaagcttca gctcaccgag gccagcccgc tcggcgatcg
     2101 gctcgaacgg ttcacgaatg acctcgtgaa caaggccgag atcgacctgg cggcgagcat
     2161 cgacgccagt caggccgcct accgggcgtc ccgctgggtt gtgatcggct ttgccgtcgg
     2221 gaccaccgct ctcgcgctcg tgctgggcta cgcgatctcg tggtcgctga ttggcccggt
     2281 gaagctgatg gagacccgac tgaaactcat tgcggcggga gatctcgcgc agcgggtcga
     2341 cgtaccgaac cgcgacgagc tcggaggcct cgcggcccaa ttcaaccaga tggccatcga
     2401 gctcgaggtt cgcacgcgcg agttgacgcg cctcgtcggt gagcttcgag cgctcggtga
     2461 ggtgagccag gccgtcagct cctcgctgga gctcgagact gtgctcgaga ccatcgtggc
     2521 ccgggcggcg cagctttcgg ctgcgcacgc gggcgtcatc tacgagtacg acagcgcgac
     2581 gcaggaactt cacgtccaga gcgcctaccg cctcgacgac gatctggccg agatcatgcg
     2641 tgtggctccg ctccgtctca gcgaaggcgt cgccggaagg gcggtggcct tgcgggcccc
     2701 attccaggtc gcggacgtgc tagacaagca cgcctacgat gtcggacgca tccggaccgc
     2761 gttcgagcgg cacggctacc ggtcggtggt ggcggtgcct cttctcttcg agcagcagat
     2821 actgggagcc ctcgtggtct ggggcaagga gacggggagc ttccctcccg aggtggtcga
     2881 tttgcttcag acttttgcga accaatctgt cctcgccatc cagaacgccc ggctgtaccg
     2941 agaaattggg gcgaagagtc gccagctcgg agtcgccagc cagcacaagt cggagttcct
     3001 cgcgaacatg tcgcatgagc tgcggacgcc gctgaatgca gtcatcggat tctccgaggt
     3061 tctgatagac cgcatgttcg gtgagctgaa cgagaagcag gaggagtatc tgaaggacat
     3121 ctacgcttcg ggcacccacc tactctcgct gatcaatgac atcctcgacc tctccaagat
     3181 cgaagcgggg cggatggagc tcgagctgac ggatttccac cttcccactg ccatcgacaa
     3241 tgccctgatg ctcgtgcgcg aacgggctgg gcgacggtca atcgcacttc atacaagcat
     3301 cgatcaccgg tttggccaca ttcaggccga tgagcgcaaa gtccggcaag tcgtgctgaa
     3361 cctgctatcg aatgcgatca agttcacgcc agagggcgga cggattgaag tcggggccca
     3421 gccaaaggat cggttcatag aggtctcggt cagcgatacc ggagtgggca tcgccccgga
     3481 ggatcaggag gcggtgttcg aggagttccg ccaggtcggg gtggcggcca agaaggtcga
     3541 gggcaccggg ctggggctta ccttgtgtag gaagtttgtg gagctccacg gcgggcggat
     3601 ctgggtgacg agcgagattg gagtaggctc gactttcacg ttcacgatcc cggtacgcct
     3661 gggcgaatga gttgatttga ttcgccgccg actcatcctg ggtggtttat gcagcgttcc
     3721 ttccatgccg cgttatgcgc acgcgcagca ctcgtcaagg acgtatcgca tcgggtatct
     3781 ctccacggcc cagtcccaca accccatcga cgccgccttt gacgaagcca tcgctggatt
     3841 tggctacgtt gaacggcaaa cctcgtgatg gatcgccgct acacgaacgg agaggctaat
     3901 cgctatcctg gtgccgccgc cgaactcgtt agtctcaagc cagattgcct cgtcgagtga
     3961 acgccggcag gtgccgtagc actacgggat gtgaccaggt caacccccgt cgtgttcctc
     4021 ggcggaatcg acccgatcag agccggcctc gtcaaaagcc tggctcggcc ggggggtaat
     4081 ttgaccggcg tgtccatcga tccagacgaa cacgtggcgt caaagcgttt gcagctattc
     4141 ggggagatga tccgaggtct ctctcgggtg atggtgctgg gatcagagca ggaactcgtc
     4201 ggcctcgagt tggcccggat tgaaagcgcg gcgcagcaac ttcacatcgc gctacaaatc
     4261 gagcgagtgc gctccggaca agacctcgag ggcgcctttt cccgcagcaa acacagggac
     4321 gcgaacgggt tcgttgtcct acgcggcggg ttcgcttacg tccatcgcca agcaatagcc
     4381 gatctggcaa cgcgataccg cctccccgga atgaacccat tccgagagaa tgtggaggtt
     4441 ggcgggctca tgtcatacgg agcaagcatt actgagattg ctcgacaggg cgcggcctac
     4501 gttgcaaagg tactgagggg ccgccaatcc tggcgatttg cccgtcgagc agccaacgaa
     4561 gtttgaactg gtggtcaacc tcaagaccgc gaaggcgctg
//
LOCUS       DASRJQ010000030         2044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3077280, whole genome shotgun sequence.
ACCESSION   DASRJQ010000030 DASRJQ010000000
VERSION     DASRJQ010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3077280"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /locus_tag="VFB89_00705"
     CDS             <1..1145
                     /locus_tag="VFB89_00705"
                     /inference="COORDINATES: protein motif:HMM:NF018452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HZM25857.1"
                     /translation="YAPLLLDLTDGPMVIDMPPGPLICVAMDLNQRWVADMGIPGPDA
                     GKGGKHLLLPPGYKGEVPAGYHTATSTTYRMLVGVRSLPVGGDVPAAIERIKTVKVHP
                     LNPSPGWMEPTWLDLTPPPQDTTPLAWENNLQYWQVLHEVVDSEPLFALYRDHYGELA
                     ALGIAKGQPFAPDARMQRILEQAAQMASAQMRVESFADRRPDRIVWKDRQWEWAALRF
                     EDGDFNAPNYADTYARDKWYFQAIGSSPAMFRRDAGAGSLYWLGLRDKAGVYLDGGKT
                     YKLTVPQPVPGKLFWSVTVYDAETRSQVQTDQGKAALRSMFELKDKAAETSVDLYFGP
                     KAPAGREGEWIKTSSGKGWFVYFRIYGPEQPAFDGSWKPGDFEAVH"
     gene            1176..1454
                     /locus_tag="VFB89_00710"
     CDS             1176..1454
                     /locus_tag="VFB89_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25858.1"
                     /translation="MKTKLANVALAVVLALTATHHIAAQAPASPASASAIAVSDQADA
                     DGADHGREAGRVPLFAAVQHGTHYSNIARLPPLMQRVNQGRALDHKAV"
     gene            complement(1482..>2044)
                     /locus_tag="VFB89_00715"
     CDS             complement(1482..>2044)
                     /locus_tag="VFB89_00715"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZM25859.1"
                     /translation="VWDVGREAQRAVESGNVDRVHRILLASAGIPGAFPFREIDGSLY
                     VDGGVTGNILYGGRVREEQSFPALWVAAYPHLPVPTIRYWVIFNNQLRPLPQVTEPTW
                     PAVVSRSLEMGTRAATLTSMRHLFAQAAISRLMRGATIEVRIVAVPDDFVPPKPGTFD
                     KDTMNALADLGEKMGEDASSWRTESP"
BASE COUNT          386 a          675 c          629 g          354 t
ORIGIN      
        1 cttacgcccc gctattgctg gatctgacgg acggcccgat ggtgatcgac atgcctccgg
       61 gaccgctgat ctgcgtcgcg atggatctga atcagcgctg ggtcgccgac atgggcatcc
      121 cagggcccga tgcgggcaaa ggcggcaaac acctgctgct gccacctggg tacaagggcg
      181 aagtgcctgc cggctatcat accgccacgt ccaccacgta tcgcatgttg gtcggcgtgc
      241 gctcgctccc cgtcggtggc gacgtcccgg ccgccatcga gcgcatcaag accgtcaagg
      301 tgcatccctt gaatccgtcc cccggatgga tggagcccac gtggctggac ttgactcctc
      361 cgccgcagga cacaactccg ctcgcctggg aaaacaatct gcaatactgg caggtgctgc
      421 acgaggtcgt cgattccgag ccgctcttcg cactctaccg ggaccactat ggcgaactcg
      481 cggcacttgg catcgccaag ggccaaccgt tcgcccccga cgcccgcatg caacgcatcc
      541 tggagcaggc cgcgcagatg gccagtgccc agatgcgggt ggaatccttc gccgatcgcc
      601 ggcccgatcg catcgtttgg aaagatcgcc agtgggaatg ggccgccctg cgtttcgagg
      661 acggagattt caacgccccg aattacgcgg acacttacgc ccgcgacaaa tggtatttcc
      721 aggcgatcgg ttcctcgccg gccatgttcc gccgcgacgc gggcgccggc tcgctctact
      781 ggttgggcct gcgcgataag gcgggcgtct atcttgacgg tggcaagacc tacaaactca
      841 cggtgccgca gccggtgcca ggcaagctgt tctggtcggt gacggtgtac gacgccgaaa
      901 cgcgcagcca ggtccagacg gatcagggca aggcggccct gcgctcgatg ttcgagttga
      961 aagacaaggc ggcggaaaca tccgtggacc tctatttcgg cccgaaagct cccgctggac
     1021 gcgaaggcga gtggatcaaa accagctccg gcaaaggctg gtttgtgtat ttccgcatct
     1081 atggtccgga gcagcccgcc ttcgacggca gttggaaacc gggggatttc gaagcggtgc
     1141 actgaacctc gacatcacaa cgaaaagaat cacacatgaa aaccaaactc gcaaacgttg
     1201 cgctggctgt ggtgctggcg ctcactgcga ctcatcacat cgcggcacag gctcccgcgt
     1261 cgcccgcatc tgcgtcagca attgccgttt ccgatcaagc tgacgccgat ggggccgatc
     1321 acggtcgcga agccggtcga gttccgttgt ttgccgcggt gcagcacggc actcattatt
     1381 caaacattgc tcgccttcca ccgctgatgc aaagagtcaa tcagggcaga gccctcgacc
     1441 acaaagctgt atgagcatcg tcagccatga cgcacacggc gtcacggcga ttctgtgcgc
     1501 caactcgacg catcctcgcc cattttttcc cccaggtcgg ccagggcgtt catcgtgtcc
     1561 ttatcgaatg tgccgggctt ggggggcaca aagtcatcgg gcacggctac gatgcggact
     1621 tcgatcgtcg cgccgcgcat cagccgcgag atcgccgcct gcgcaaacag gtgccgcatg
     1681 gacgtgagcg tggccgcgcg cgtgcccatt tcgaggctgc gcgagaccac ggccggccag
     1741 gtcggctccg tcacctgggg cagcggccgg agctggttgt taaagatgac ccagtagcgg
     1801 atcgtcggca ccgggaggtg cggataggcg gccacccaga gcgccgggaa gctctgctct
     1861 tcgcggaccc ggccgccata gagaatgttc ccggtcacgc cgccgtccac gtagagcgag
     1921 ccatcaatct cgcggaacgg gaaggcgccc gggatacccg ccgaggcgag cagaatccgg
     1981 tgcactcggt ccacgttacc gctctccacc gcgcgctgcg cctcgcgacc gacgtcccac
     2041 accc
//
LOCUS       DASRJQ010000031         2215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3639685, whole genome shotgun sequence.
ACCESSION   DASRJQ010000031 DASRJQ010000000
VERSION     DASRJQ010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3639685"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VFB89_00720"
     CDS             <1..244
                     /locus_tag="VFB89_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25860.1"
                     /translation="VYARLAAVFSVVQVGMFTLLVWVPIVTAGPSASDWNEFVVSWAL
                     TAAAWVVADSYRGMRWLATRRSAMRTESRGTGASAR"
     gene            414..1025
                     /locus_tag="VFB89_00725"
     CDS             414..1025
                     /locus_tag="VFB89_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HZM25861.1"
                     /translation="MFLEHGNQRPRVARDAWVAPTAVLCGDVRVGSGSCVAFGAVLVA
                     EGMPVVIGQQTIVREHALLRATAEHPLTVGDYVLVGPRAALYGCRIEDEVFLATGTTV
                     FHGAQVGRGAEVRINGVVHVRTRLPAGGLVPIGWVAVGDPAEILPPSEHDRIWAIQRS
                     LDFPRVVYGLDRKPDGSVDMRELTARVAMGLAGHRQDRVVGEG"
     gene            1220..1690
                     /locus_tag="VFB89_00730"
     CDS             1220..1690
                     /locus_tag="VFB89_00730"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HZM25862.1"
                     /translation="MPESSTDAPRVVAFPPLLYAGTLALGLILHWLMPRHPLPPVPAR
                     LLGGLLLIASAALARWGERTMRRAGTNVDPRQPALALVVDGPFRFSRNPLYLGLTGFY
                     LAITLLVNALWPLLLLVPLLGVAHWGIIKREERYLEGKFGEPYRAYRSRVRRWL"
     gene            1941..>2215
                     /locus_tag="VFB89_00735"
     CDS             1941..>2215
                     /locus_tag="VFB89_00735"
                     /inference="COORDINATES: protein motif:HMM:NF022963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3224 domain-containing protein"
                     /protein_id="HZM25863.1"
                     /translation="MKKRASARFTIKNWDEKAVSEGPDLPKLSRATVTKTFTGDIEGE
                     SQVEYLMLYRSDGSATFVGLERVVGRVAERAGSFVLQRTGVFEGGEA"
BASE COUNT          360 a          661 c          777 g          417 t
ORIGIN      
        1 cgtgtacgca cggctggcgg ctgtgttttc agtggtgcag gtgggcatgt tcacgctgct
       61 ggtgtgggta cccatcgtga cggcagggcc cagcgcctcg gattggaatg agttcgtggt
      121 ctcttgggcg ttgacggccg ccgcttgggt ggtagcggat tcctaccgcg gcatgcgttg
      181 gctcgctact cggaggtcag caatgagaac cgaatcacga ggaacgggcg cgagcgcccg
      241 gtaacttcac cgtaacgccg tcgcagcgtg cttgacagca ctcgggtgcg aggcatactg
      301 ggcaactcga ctgcagaatc cgttcacccg aggcaggagc ccagatgttc cgcgcatgcc
      361 tggctgaagc gtaggtcgtt aggcgccaag tcccggggtt ccggagtccc tcgatgttcc
      421 tagagcatgg caaccaacgc cctcgcgtcg cccgggacgc ctgggtcgca cccaccgcgg
      481 tcctctgcgg ggacgtgcgc gtaggtagcg gcagctgcgt cgcgttcggc gcggtcctgg
      541 tcgccgaggg catgccggtc gtcatcggcc agcagactat cgtccgcgag cacgccctgc
      601 tccgcgccac agcggaacac ccgctcaccg tcggggacta cgtcttggtc gggccacggg
      661 ctgcgctcta tggctgccgg atcgaggacg aagtcttcct ggcgaccggg accaccgtgt
      721 tccatggcgc ccaggtcggc cgcggcgccg aggtgcgcat caatggtgtt gtgcatgtgc
      781 ggacccgatt gccggccggc gggctcgtgc cgatcgggtg ggtcgcggta ggggatcccg
      841 cggagatctt gccgcccagc gagcacgacc gaatctgggc catccagcgc tccctcgact
      901 tcccgcgcgt ggtctatggg ctggaccgga agcccgatgg ctcggtggac atgcgcgagt
      961 tgacggctcg agtggccatg ggcttggctg ggcatcggca agaccgggtg gtcggggagg
     1021 gctaggccgc ctcatgccct tattagcgga agaagcacgc cgatcagcac gcttgcacca
     1081 tccatcaggg catgcgcaat cgccgaacgg aatcaaccgg cgaagctgca ggggcgacct
     1141 tcgtagagag tggcgcctga ccaccgtctg cggttaacag gagtgcgtcg cgctttcgtt
     1201 aggcaggatc acaggagcta tgcccgaatc gagcaccgat gcaccaagag tcgtcgcctt
     1261 tccgccactg ttgtatgcgg gcacactcgc gctggggctg atacttcact ggctgatgcc
     1321 gcgacatccg ctgccgccgg tgccggcacg tctcttgggt ggtctgctcc tcatcgcgag
     1381 tgccgcactg gctcgctggg gcgaacgaac catgcgaagg gccggcacca acgtggatcc
     1441 gcgtcagccc gcgctggcgc tggtcgtcga tggccccttt cgattttccc ggaatccgct
     1501 ctatctcggg ctcaccgggt tctacctggc gattacgctg ctggtcaacg cgctgtggcc
     1561 cctcctgctc ctggtgccgc tgttgggggt ggcgcactgg gggatcatca agcgggaaga
     1621 acgctatttg gaaggaaagt ttggtgagcc gtatcgggcc tatcggtccc gggtgcggcg
     1681 gtggctatag gtaattgggc atcgggagat gctggatggg cgtagtatca ccgcttcgcg
     1741 ggggcccagt gcttgagatc ggttcccggc gttcacacca cggatgaccg ttcactctga
     1801 ccgcagccta accgtagaaa tcgttgcggg tgtcaatacc ggtttctccg acacagtcga
     1861 ccatggggca gtcgcggggg gcttggtggt tcagctccgc tcggaggcgc ctctcccgat
     1921 tacccggaca aggtgggcac atgaagaagc gtgcgagcgc tcggttcacg atcaagaact
     1981 gggatgagaa ggcggtgagt gagggaccgg atctgccaaa gcttagccgg gccacggtga
     2041 ccaagacgtt caccggcgat atcgagggcg agagccaggt cgagtacttg atgctgtacc
     2101 gaagtgacgg ctcggccacg ttcgtcggac tggagcgggt cgtcggccga gtcgctgaga
     2161 gggcgggtag ctttgtgctc caacgcaccg gggtattcga ggggggcgag gctaa
//
LOCUS       DASRJQ010000032         4757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1952285, whole genome shotgun sequence.
ACCESSION   DASRJQ010000032 DASRJQ010000000
VERSION     DASRJQ010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1952285"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            391..463
                     /locus_tag="VFB89_00740"
     tRNA            391..463
                     /locus_tag="VFB89_00740"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:424..426,aa:Ala,seq:cgc)
     gene            493..565
                     /locus_tag="VFB89_00745"
     tRNA            493..565
                     /locus_tag="VFB89_00745"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:526..528,aa:Gln,seq:ctg)
     gene            696..3386
                     /locus_tag="VFB89_00750"
     CDS             696..3386
                     /locus_tag="VFB89_00750"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM25864.1"
                     /translation="MTDLRTQLQTALATSYTLERELGRGGMATVFLAQDLRHDRPVAL
                     KVLHPDLARTLGPERFQREIKLAARLQHPHILTVHDSGEAAGQLWFTMPFVEGESLRD
                     RLRRERQLPVEAALRIATEAARALEYAHRHGVVHRDIKPENLLLTADGATLVADFGIA
                     RALAGVDERLTETGLAIGTPAYMSPEQAAGDKALDARTDIYSLGAVLYEMLAGEPPFT
                     GPTAQAIIAKRFSGEVPRVRQARPSVPEQVDAAVARALAPVAADRFNSAEEFARALTT
                     TPVTSAAAAAAPVPSAAPEAAASSRVTTPEPTRSPARRVPVAALALVLGFLVGLGVLF
                     AWRRTHPDAGEDTGPKRLAVLPFENLGDSADAYFAEGITNEVRGKLSQVSGLAVLARA
                     SSNEYRATSKAPQQIARELGAEYLLTATVQWQKAPGGPSRVRVSPELIRVEPGAAPTT
                     KWQQPFDASLTDVFQVQADIAMKVASALNAALGDSVRHELAAKPTENLAAYDAYLKGE
                     AASQALAAGDPPSLRRAVGFYQQAVALDSGFVPAWAQLGRARAVLYANSTPAPELAAQ
                     ARDAANRSRALGPDRPEGQLALGEFYRSVQVDNRQALAAYEAGLKLAPTNVDLLVGAA
                     LAEQSLGHWEPSLRHLTQAAALDPRAANTARRTTRTLLYLRRYPEAQAAADRSLMVAP
                     TNLQIIEQRAMVALAQGDLAGAQAVVRAALPNVDPAALLAFFGNFQDLYWVLEDAEQQ
                     QLLTLPPSAFDDDRGTWSAVRAQTYGLRKDLAKARVYADSAQLAFREQLRATPDDDQR
                     HVLRGLALAYLGRKAEAIEEGERGVALLPVSRDAFAGAYTQHQLVRIYLLVGEPEKAL
                     DQLEPLLKMPYYLSPGWLKIDPTFAPLKGNPRFERLVAGG"
     gene            3929..4531
                     /locus_tag="VFB89_00755"
     CDS             3929..4531
                     /locus_tag="VFB89_00755"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZM25865.1"
                     /translation="MSRLLGLGERFPEFSMHACVSSEPGSEFRIVDAHNLEGKWAAIF
                     FWPLDFTFVCPTELVEFNRSLPQFRALNTRVFGVSGDSHYVHLAWRKENPTLRNLEFP
                     MLADYRKELAEELGILDPIEKVPLRATYLVDPERTIRWLCVNDLSVGRSTGEVLRVLE
                     ALQSGELCPANWSPGQATLERPTDTEKGVRWETSEHLALR"
BASE COUNT          780 a         1583 c         1607 g          787 t
ORIGIN      
        1 gttctcgtag agcgggacgg acccggcgtc cgtggtgggc atgcccgcct tgaaatggtg
       61 ctggtggtct ggctcctggg cctggacctg gtaagcggca agggcggctg cggcgaagac
      121 cgcggccacc acccgaccca gcatccggcg gaacatcgct tcccgcatac cgtctccttg
      181 gcaaagtggc gcgtccgcaa cgacttcccg atggcgggct agcggtgccg ccgcagggac
      241 gctccgggga cgcaccgtgg attgcgcggg ggatcaaggg gaccggacag tggagcagga
      301 cgggcggtgt ccgcgctctc cgccgtgccc ccaatctact gtccggtcgg ttgatggaga
      361 gggggtaagg tgctagtttt ccggaccttc ggggctgtag ctcagctggg agagcgctgc
      421 aatcgcactg cagaggtcag gggttcgatc cccctcagct ccatggcgtc atccagagac
      481 gccacgattg tctgcccctt ggtgtaactg gcaacacgcc tgactctgga tcaggagagt
      541 cctggttcga gcccaggagg ggcaatgccc accgtgaaca acggtgggcg tttgtttttg
      601 ggcctcggct tcggctcggg cctagcccag gggtcgtgct ggtctccggg ccgcggggcg
      661 ggtacctttg accgaccctc aatacatcag gggcggtgac ggacctccga acgcagctgc
      721 agacggcgct cgccacgtcg tacaccctcg agcgcgagct cgggcgcggt ggtatggcga
      781 ccgtcttcct cgcccaggac ctgcggcacg accggccggt cgcgctcaag gtgctgcacc
      841 ccgacctcgc ccgcacgctc ggccccgagc ggttccagcg ggagatcaag ctggccgccc
      901 ggctccagca cccccacatc ctcacggtcc acgactcggg cgaggctgcc ggccagctct
      961 ggttcaccat gccgttcgtc gagggcgagt cgctccgcga ccggctgcgg cgggagcggc
     1021 agctgccggt ggaggcggcg cttcggatcg ccaccgaggc ggcccgggcg ctcgagtacg
     1081 cgcaccgcca cggcgtagtc caccgggaca tcaagccgga gaacctgctg ctcaccgccg
     1141 acggggccac gctggtcgcc gactttggca tcgcgcgggc actggcgggc gtagacgagc
     1201 ggctcaccga gacggggctg gcgatcggga ccccggccta catgagccct gagcaggcgg
     1261 cgggcgataa ggcactcgac gcacgcaccg acatctactc gcttggcgcc gtgctgtacg
     1321 aaatgctggc cggcgagccg ccattcaccg gtcccacggc acaggcgatc atcgccaagc
     1381 gcttcagtgg cgaggtgccg cgggtgcggc aggcacggcc gagtgtgccg gagcaggtgg
     1441 acgcggcggt cgcgcgcgca cttgccccgg tcgcggcgga ccggttcaac tcggcggagg
     1501 agttcgcccg ggcgctgaca acgactccgg tgaccagtgc tgccgccgcg gcggcacccg
     1561 tgccgagcgc cgcccctgag gccgccgctt ccagtcgagt gacgacaccc gagcccaccc
     1621 ggtcgcctgc gcgaagagtt ccggtagctg cgctcgcgct cgtcctcggc tttctcgtcg
     1681 gactgggggt gctcttcgca tggcgacgaa cgcaccctga cgccggggag gataccgggc
     1741 ccaagcgcct cgccgtgctc ccgttcgaga acctgggcga ctcggccgat gcgtacttcg
     1801 ccgaaggcat caccaacgaa gtgcgcggca agctctccca ggtgtcgggg cttgccgttc
     1861 ttgcccgggc cagctcgaac gagtaccgcg cgacgtccaa ggcgccgcag cagattgccc
     1921 gagagctcgg ggccgagtac ctcctcacgg caaccgtgca gtggcagaag gcccccggcg
     1981 gcccgagccg ggtccgggtc agccccgagc tcatccgggt cgagcccggg gccgcgccga
     2041 ccaccaagtg gcagcagccg ttcgacgcct cgcttaccga tgtcttccag gtccaagcgg
     2101 atatcgcgat gaaggtcgcc agcgcgctca acgcggcctt gggcgacagt gtgcgtcacg
     2161 aactcgcggc caagccgacg gagaacctgg ccgcctacga tgcctacctc aaaggcgagg
     2221 ccgcctcgca ggcgctggcc gcgggggacc cgccgagtct gcggcgggcc gtcgggttct
     2281 accaacaggc ggtcgccctc gattccgggt tcgtcccggc ctgggcccag ctcggccgcg
     2341 cgcgcgcggt cctctacgcc aatagcacgc cggcgcctga gttggcggcg caggcccgtg
     2401 acgctgctaa tcggtcgcga gcgctcggcc cggatcggcc cgaagggcag ctggcgctcg
     2461 gcgaattcta tcggtctgtc caggtcgaca accgccaggc actcgcggcc tacgaggcgg
     2521 ggctcaagct ggcgcccacc aacgtcgacc tcctcgttgg cgcggccctg gccgagcagt
     2581 ccctgggcca ctgggagccg tctctccggc acctcacgca ggcggctgcg ctcgatcccc
     2641 gcgcggccaa cacggcccgg cgcactaccc gcacgctgct ctacctacgc cgctatcccg
     2701 aggcgcaggc ggctgcggac cgcagtctca tggtcgcccc aaccaacctc caaatcattg
     2761 agcaacgggc gatggtggcg cttgctcaag gggatctcgc cggcgcccag gctgtcgtac
     2821 gtgccgccct ccccaacgtg gacccggccg cgctgctcgc gttcttcggc aactttcagg
     2881 atctctactg ggtgctagaa gatgctgagc agcagcagct cctcaccctt ccgccgagtg
     2941 cttttgacga cgaccgggga acctggagcg ccgtacgtgc gcagacgtat gggttgcgca
     3001 aggacctggc caaggcgagg gtgtatgccg actctgccca gctcgccttc cgggagcagc
     3061 tccgggccac gccggacgat gaccagcggc atgttttgcg gggcttggcc ctcgcgtatc
     3121 taggccgtaa ggccgaggcg attgaggaag gggagcgggg cgtggcgctg ttgccggtca
     3181 gccgggatgc gttcgccgga gcctacactc agcaccagct cgtccgcatc tacctgctcg
     3241 tgggggagcc cgagaaggct ctcgaccagc tcgagccgct gctgaagatg ccgtactatc
     3301 tctcgcccgg ctggctcaag atcgatccca ccttcgcacc gctcaagggc aacccgcggt
     3361 tcgagcgttt ggttgccggc ggataatctg cacttcgcgt cgcggtaccg ctgaagttga
     3421 tgaaagctgg gacaggaatc aggaacatcg gcaaacttcg acatctgtgc gagcaaacca
     3481 cagcaaagtc gagcattcaa gccagcaaac tccagcatcg ggtgacacga actcgcgtaa
     3541 aagtcagcaa gttgctggag ttcagggggt tacaacggcc cctctggatc aggagagtcc
     3601 tggttcgagc ccaggagggg caatacggcc gcagaaagcg cggccgtatt ttttgttggg
     3661 gctcggcctt cggctcgctg ccgccggtcc ttgacggcgg ttgcaccgaa ccgtaccgtg
     3721 gacttgctaa accacgtttt gccccgtcga caaggcgcac ccgggaagcc gtggccgcaa
     3781 cgtcagggat cctcaccgct cgacagcgta gcgattgccc gcgcgggaga gtgaagaccg
     3841 ccgacgccca ccacggggtc ggcggcgatt gtattgctgt tgccgcgggg gcgccctgac
     3901 ggttccacct tgtgcgattg gaggtcccat gtcgcgacta ctcggcttgg gggagcgctt
     3961 ccccgagttc tccatgcacg cgtgcgtctc atccgagcca ggatcagaat tccgaattgt
     4021 cgatgcgcac aacttggagg ggaagtgggc tgcgatcttc ttctggcccc ttgacttcac
     4081 ctttgtgtgc cccaccgaac tcgtggagtt caaccgctcg ctcccgcagt ttcgcgccct
     4141 caatacgagg gtgttcggcg tgagtgggga cagccactac gttcacctgg cgtggcgcaa
     4201 agagaacccg actcttcgca atctcgaatt cccgatgctg gcagactatc gcaaggagct
     4261 cgccgaggag ctcgggattc tggatccgat tgagaaggtt cctctacggg caacttacct
     4321 cgttgacccg gagagaacga ttcggtggct ctgcgtgaac gacctgtcgg tgggccggag
     4381 cacgggggaa gtactgcggg tgctcgaggc gctccagagc ggcgagttgt gcccggccaa
     4441 ctggagcccg gggcaggcga cgcttgagcg accgacggac accgaaaagg gggttcggtg
     4501 ggagacgtcc gagcaccttg ccctccgctg acgccggagg gagtcttcga gagcaggtgg
     4561 ctgaaatgtc tttctgatgc gacatgattt cgccccgtcg cggaattggc cgggcccgca
     4621 ttctcacgcc gagtagccag cgcgtcatcc ggactagatt ccaggtcgtc tgctctcagg
     4681 ggtgggctgt taaggtcgag aaggttggca aacttggaca tctgagtgag caaaccacag
     4741 caaagtcgag cattcaa
//
LOCUS       DASRJQ010000033         4255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_818891, whole genome shotgun sequence.
ACCESSION   DASRJQ010000033 DASRJQ010000000
VERSION     DASRJQ010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818891"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VFB89_00760"
     CDS             complement(<1..369)
                     /locus_tag="VFB89_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZM25866.1"
                     /translation="MRPAPTAPVVTPPGVRRLGLGLAALGRPGYITVEHARDLAGNYE
                     PGAMEQRTHAVLDAAYAGGVRYFDAARSYGRAEDFLASWLANRRIVPGAVVVASKWGY
                     TYTAGWRVAAERHEVKDHSLA"
     gene            504..878
                     /locus_tag="VFB89_00765"
     CDS             504..878
                     /locus_tag="VFB89_00765"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HZM25867.1"
                     /translation="MPLTDMERRHLERRLREERAQVVRILDRYKDEQSAAERDRAGDV
                     STFPTHPADEGTDTYDADVEASLAARATEELHEIDAALERLYRDPERFGVCERTGDPI
                     PFARLDLIPWARTCDQERRHGR"
     gene            complement(1063..1752)
                     /locus_tag="VFB89_00770"
     CDS             complement(1063..1752)
                     /locus_tag="VFB89_00770"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HZM25868.1"
                     /translation="MFIGWAGHRLNRRVQPSMWENGMSQQIRPYEPLTSDNAALVLVD
                     HQVGLMTGVRDYSTGELKHNVVALAKAAQALKLPIIVTTTARDSMWGPTFPELVEALP
                     GVEIIDRSSVNAFDDERVAHAIAATRRKKLIFAGISLEVCAAFPAITAIGKGLEAYVA
                     VDASGTFSETKRQVGLLRMQQAGVILSDYATLMVEILKDNARPEAGEVYGAMDMPWAK
                     LVGQIAQAYGK"
     gene            1834..2469
                     /locus_tag="VFB89_00775"
     CDS             1834..2469
                     /locus_tag="VFB89_00775"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZM25869.1"
                     /translation="MKKPPPARRKGRKGRPPLQTAVSHAAIMDAVYALLQEKSVRDLT
                     IEEVARRAKVGKPTIYKWWSTKATLVLAMLCERMAPNLERPTVLTAQESLRFRVRRLI
                     DAFNGPFGKIVAGLIAEGQSEPAILHEFFDRWVSPRRNATIADLQRGKNAGELPSETE
                     PELLNDAVFGAIYYRLLLRSGPLTKRFGEDLVEHVLRGQCARALNQLGEAN"
     gene            complement(2448..3083)
                     /locus_tag="VFB89_00780"
     CDS             complement(2448..3083)
                     /locus_tag="VFB89_00780"
                     /inference="COORDINATES: protein motif:HMM:NF013209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZM25870.1"
                     /translation="MTQPLWSQRFFASTRGRIVALLRRTPMTVDQLAQALDLTDNAVR
                     AQLAGLERDGIVQPEGIRKGTSKPSVAYALTPDVEPLLSRAYRPLLDALLSTLAERLP
                     DREQAAMLRATGRALGRRVAVPRSDLAARLHAAAAVLNDLGGLAAAERGEDGWAIRSE
                     GCPVSGLVRQHPRVCKAVEAMVAELVGETVREHCDRSGDRPRCHFQLASPS"
     gene            3187..3732
                     /locus_tag="VFB89_00785"
     CDS             3187..3732
                     /locus_tag="VFB89_00785"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HZM25871.1"
                     /translation="MLTLALAHAGLVTLLATGPSVPTPVPPPQAALDDATIVAIFDAA
                     NTYDMETSGLAEKRAKHDDVRALAKQFAGDHKAVRQQGRDLAKKLNVTPTPPKDDKSA
                     RDHADAMKKLESAKGEDFDRAYLAHEVAYHEAVINAINQTLLPAIKNPELKAFVQKVG
                     PAFQAHLDAAKKLAQKYGATS"
     gene            complement(3741..>4255)
                     /locus_tag="VFB89_00790"
     CDS             complement(3741..>4255)
                     /locus_tag="VFB89_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25872.1"
                     /translation="NCFLRIPKDTDLRPVVTGWYSEFTALEERKREERVVYGRAGDRF
                     AGATYDPTSHYRAAAVFRFFQDRDLTPPLLREVSQHQIGLLAREFDALDLDPAIISRD
                     PAPLSEIGGFLALRSLTATRLTRELHARGVRTDARGNILRLGPAPYLSDRQLRDAIAL
                     LREVVRERPG"
BASE COUNT          811 a         1390 c         1333 g          721 t
ORIGIN      
        1 tgccagcgag tggtccttca cctcgtgccg ctcggcggca acgcgccacc ccgcggtgta
       61 ggtgtagccc cacttcgagg cgacgaccac ggcgcccggc acgattcgcc gattcgccag
      121 ccacgacgcg aggaagtcct ccgcccggcc gtaggatcgc gccgcgtcga agtagcgcac
      181 gcccccggcg taggcggcgt cgagcaccgc gtgggtacgt tgctccatgg cgcccggttc
      241 gtaattcccc gccaggtcgc gcgcgtgctc gaccgtgatg tacccggggc gtcccagggc
      301 cgcgagcccg agcccgaggc gacgaacacc gggcggtgtc acaactggag ctgtgggtgc
      361 cggacgcatt gtggatcgcc tcacatgtct caaatgctgc ctatttgccg gctctgcggc
      421 agcgtccccc gtggaccgct gagcggcgct cgagcgccca cccgcatccc gactggcaca
      481 cccgttaccg aatgggagag cacatgccct tgactgacat ggagcgccgc cacctcgagc
      541 gccggctccg cgaggagcgc gcgcaagtcg tccgcatcct cgaccgctac aaggacgagc
      601 agagcgcagc ggagcgcgac cgcgccgggg acgtctcgac gttcccgacc catcccgcgg
      661 acgaggggac cgacacatac gacgcagacg tcgaggcgtc gctcgctgcg cgggctacag
      721 aggagctgca cgaaatcgac gccgcgctcg agcgcctcta tcgcgacccc gaacgcttcg
      781 gggtgtgtga gcgaacgggg gacccgattc cgttcgcgcg gctcgacctc atcccctggg
      841 cgcgaacctg cgatcaggag cggcgccacg gccgatagtc ctggaggact tgggccaaat
      901 cggtcgattg cctcgaataa tgcgcgactc agctaggacg ccttctgggc cgcttcgcgg
      961 taacgactcg acactgtcca tcaagagact gtagctgtga tagtggtcgt ggaagccgcg
     1021 cccaagtcct tcccggtcgg caggctcctc tgaatgggtt gttcacttcc cgtacgcctg
     1081 ggcgatctgg ccaacgagct tggcccaagg catatccatc gccccgtaga cctcgccggc
     1141 ctccggccgc gcattgtcct tgaggatttc aaccatcaga gtcgcgtagt cagagagaat
     1201 tacgccggcc tgctgcattc gcaggagccc gacctggcgc ttggtctcgc tgaaagttcc
     1261 cgaggcatcg accgccacat aggcttccag ccccttacca atggcagtga tggccggaaa
     1321 ggccgcgcag acttcgagag agataccggc gaagatgagc ttcttacggc gggtcgccgc
     1381 gatggcgtga gccacgcgct cgtcgtcgaa ggcgttgacc gacgagcgat cgatgatctc
     1441 cacgcccggc agtgcctcga cgagctcggg gaacgtcggc ccccacatgc tgtcgcgggc
     1501 agtcgtcgtg acgataatag gcaacttcag cgcctgggcc gctttggcca aggctaccac
     1561 gttgtgcttc aactcgccgg tcgaataatc acggacgccc gtcatcaagc cgacctgatg
     1621 atcgacgaga acgagtgccg cgttgtcgga agtgaggggc tcgtaagggc ggatttgctg
     1681 gctcatacca ttctcccaca tgcttggttg aactcttcga ttcaatcggt gaccagccca
     1741 gccgataaac attttccttt acgttccgtt ttataatagc agggtgaccc gggtgtcaat
     1801 agactttacg atacgttgtg ttaaactatt ttcatgaaga aaccaccccc tgctcgccgc
     1861 aaaggccgca aaggccggcc gcccctgcaa acagcggtat cacatgccgc catcatggac
     1921 gcggtctacg ccctgcttca ggagaaatcg gtccgcgatt tgaccatcga ggaggtcgcg
     1981 aggcgagcca aggtcggcaa acccaccatt tacaagtggt ggtcgaccaa ggcaacgttg
     2041 gtgttagcca tgctctgcga gcggatggct cccaacttgg agaggcccac ggtcctgacc
     2101 gcgcaagagt cgctgcgctt tcgagtgcgg cggttgatcg acgcgttcaa tgggcccttc
     2161 ggcaagatcg ttgccgggct aatcgccgag ggccaaagcg agccagccat tcttcacgag
     2221 ttctttgacc gatgggttag tccccggcgg aatgctacca tcgccgatct gcagcgaggc
     2281 aagaatgcag gcgagttgcc gtcggagacc gaacctgaac tgctgaacga cgcggtcttt
     2341 ggcgcgatct attaccggtt gttattgcgc tcgggcccat tgaccaaacg gttcggcgag
     2401 gatttggtcg agcacgtcct tcgggggcag tgcgccaggg cgttgaatca gctcggggaa
     2461 gccaattgaa aatggcacct cggccgatcc cccgagcggt cgcagtgttc gcgcacggtc
     2521 tcgcccacca gctcggccac catcgcctcg acggccttgc ataccctggg gtgctgccgc
     2581 accaggcccg ataccgggca cccctcgctc cggatcgccc acccatcctc gccccgctca
     2641 gcggccgcca gcccgcccag gtcgttcagg actgcggcag ccgcgtggag tcgggccgcc
     2701 aggtcgcttc gtggcaccgc aaccctgcga cccagtgccc gtccggtcgc tcgcagcatg
     2761 gccgcctgct cccgatccgg gagccgctcg gcgagcgtgc ttagcaaggc gtcaagcaaa
     2821 ggccgatagg cccgggagag caggggctcg acatccggag tcagggcata ggcaacggaa
     2881 ggcttgctgg tccccttcct gatcccctcc ggctgcacga tgccatcacg ctccagcccg
     2941 gccagctggg cgcgcacggc gttgtctgtc agatcgaggg cctgggccag ctggtctacc
     3001 gtcatgggtg tgcggcggag cagagcgaca atgcggcccc gggtcgaggc aaagaagcgc
     3061 tggctccaaa ggggctgtgt cacgcggcgt ctcccggtgc aaggtgagac aaaagaaagt
     3121 caagtaaaat attgactttc ttccgaagat tcgcaactat ccgggcccct actcgaggag
     3181 ccgctcatgc tcacgctagc tctggcccac gcaggccttg tgaccctcct ggccaccggc
     3241 ccttcggtgc cgacgccggt ccccccgcct caggctgcgc tcgacgacgc aaccatcgtg
     3301 gcgatcttcg atgccgcaaa cacctacgat atggagacgt ccgggctcgc cgagaagcgc
     3361 gcgaaacacg atgatgtgcg tgcactggcc aagcaattcg ccggcgatca caaggcagtg
     3421 cgacaacagg gacgcgacct ggcgaagaag ctcaacgtca cgcccacgcc gcccaaggac
     3481 gacaagagcg cgcgggatca cgcggacgca atgaaaaagc tcgagtcagc caaaggtgag
     3541 gatttcgacc gagcttacct ggcccatgaa gtcgcttatc acgaggccgt tatcaacgcg
     3601 atcaaccaga cgctgctgcc tgcgatcaag aatccggagc tcaaggcatt cgtccagaaa
     3661 gtgggaccag cctttcaagc ccacctcgat gccgccaaga agctggccca gaagtacggc
     3721 gcaacgtcgt gaagcacatg tcagcccggg cgctctcgga ctacctctct cagcagagcg
     3781 atagcgtcac gtagctgccg atcggagaga tagggggccg ggccgagccg gaggatattg
     3841 cctcgggcat cggtccggac cccgcgggcg tgcagctcgc gggtaagtcg cgtagccgta
     3901 agcgagcgga gcgccaggaa cccgccgatc tcgctcaacg gagccggatc gcggctgatg
     3961 attgcgggat cgaggtccag cgcgtcgaac tcccgggcaa gcaggccgat ctggtgctgg
     4021 ctcacctctc ggagaagagg gggcgtgaga tcgcgatcct gaaagaaccg aaagacggcc
     4081 gctgctcgat aatgactggt gggatcatag gtcgctcccg cgaaccggtc gccggcccgg
     4141 ccatacacga cgcgctcttc ccgctttcgt tcctcgagtg cggtaaactc gctgtaccag
     4201 ccagtcacga ccggccggag atcggtgtcc ttggggattc gaaggaagca gtttc
//
LOCUS       DASRJQ010000034         3535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3362859, whole genome shotgun sequence.
ACCESSION   DASRJQ010000034 DASRJQ010000000
VERSION     DASRJQ010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3362859"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..712)
                     /locus_tag="VFB89_00795"
     CDS             complement(38..712)
                     /locus_tag="VFB89_00795"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZM25873.1"
                     /translation="MAVTPNRMKGFYAGLIGVLVVGAGALGWMLTRPRTTSIPANVTV
                     QASDTAGFRGYLLGSDTAKVEITEYADYQCPFCQTFDQLQMPTIKKDLIDSGRLRWRY
                     RDFPLQQHPFSRLAAHSAACADEQSKYWPQHHSIYDSQQDWAQLRDAAPKLRQYAQAN
                     GLDLGRYDSCMQSAKYAGRIQASYDEGVQVGVSATPTLMVGGRLYQGRLDSDAIARLV
                     DSLTTH"
     gene            complement(688..993)
                     /locus_tag="VFB89_00800"
     CDS             complement(688..993)
                     /locus_tag="VFB89_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25874.1"
                     /translation="MAEHDEVYDKHRADRLAELLRELAARIQELDRQGALLSGVPELL
                     RLMGDARSELFHYEVRSTYDTPEVAESRRIVEEAQRKGSFNLEEPEDEEPWRSPPTE"
     gene            1172..1456
                     /locus_tag="VFB89_00805"
     CDS             1172..1456
                     /locus_tag="VFB89_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25875.1"
                     /translation="MSKKKPRRFVATATSFDQARDELFSHILRCGVIDALPEHQKEWF
                     DDTMQYLADRYSDLSDEDLGRLRTLGERYCQPVQRRSETASRENAGSLAG"
     gene            complement(1511..1969)
                     /locus_tag="VFB89_00810"
     CDS             complement(1511..1969)
                     /locus_tag="VFB89_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25876.1"
                     /translation="MSSTWPSWVLAAAAVIPGLLAAQEAREIGVQALVTSSDPALVTG
                     GVYGAMRVAARARIALSLGGGVSDGQAAGRGELLGHFLLSPGATTRPGVYVGGGIAGV
                     VGPVDQGYVVALVGMESAPGRGSGWAVEAGVGGGFRFTAGWRWRWRRAPK"
     gene            complement(1950..2348)
                     /locus_tag="VFB89_00815"
     CDS             complement(1950..2348)
                     /locus_tag="VFB89_00815"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZM25877.1"
                     /translation="MRRVELTWEGDMRFRGGEPGGPTTLIDADNATAPGPMLTLLLSA
                     AACSGADVVSILGKMRVGLAALRIEVEGRRQAQDPKRLRSLRFTFHVKGGAVEQAKVR
                     RAVELSLQKYCSVVHSLAPDIAVEYELDLA"
     gene            complement(2368..2946)
                     /locus_tag="VFB89_00820"
     CDS             complement(2368..2946)
                     /locus_tag="VFB89_00820"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HZM25878.1"
                     /translation="MSVPDADPAAGVSRPRTSRVSRRWTAADVLTAVRLPLAVLFLLA
                     EDVWARVTIVAVAAATDLLDGPIARRFGSSAFGAVLDPIADKLFMACAFGVVAFSGRL
                     EWYEVAGVLVRDIVATVAFVVTAVSRRPSAIPARLGGKAVTVGQCLTLLAFLLDSSFL
                     RPLAWATGAVGLYAIWDYSRVAGSEKRPVGSR"
     gene            complement(2985..>3535)
                     /locus_tag="VFB89_00825"
     CDS             complement(2985..>3535)
                     /locus_tag="VFB89_00825"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HZM25879.1"
                     /translation="VVERDKAQSALAMVFRGPSRRSADRHAAEVWAAVASGLGGRIFE
                     ALRERRSLAYSVMASSWQKGRAGALVTYIATSPEREAEARSAMLAELERFAAGPVTAD
                     ELAQAINYLAGQAEVRRQHATAVAGDLLEAWLVGCGLEELENQAAAYRSVTASQIQRL
                     AEQTFKGLIRAEGVVRGSGGGR"
BASE COUNT          652 a         1212 c         1072 g          599 t
ORIGIN      
        1 ccgcccccga ggaagaggct tgggaggctc cgacacgtca gtgggttgtc agcgagtcga
       61 cgaggcgggc gatggcatct gagtcgaggc gcccttggta aagccgcccg ccaaccatca
      121 aggtaggggt cgcgctcaca cctacctgaa ccccttcgtc atagctcgcc tgaatgcggc
      181 ccgcatactt cgccgactgc atgcagctgt cgtagcgacc gagatccagg ccattggcct
      241 gagcgtattg gcggagtttc ggcgcagcat cccggagttg ggcccagtcc tgctggctgt
      301 cgtagatgct gtggtgttgc ggccagtatt tggattgttc gtccgcgcat gccgccgagt
      361 gagcggcgag ccgggaaaaa ggatgctgtt gcaggggaaa gtctcgatac cgccagcgga
      421 gccgcccact gtcgatcaag tccttcttga tcgtgggcat ctgcagctga tcgaaggtct
      481 ggcagaaggg acactgatag tcagcgtact ccgtaatttc gactttggcg gtgtccgatc
      541 ccaggaggta tccgcggaat ccggccgtat ccgatgcctg gacggtcacg ttggcgggaa
      601 tgctggttgt tcggggacga gtgagcatcc atcccaaggc gcccgccccg accaccaaca
      661 caccaatcag cccggcatag aaccccttca ttcggttggg ggtgaccgcc atggttcctc
      721 gtcctccggt tcttcgaggt tgaaggagcc cttgcgctgc gcctcctcga cgatccggcg
      781 gctttcggca acctccggcg tgtcgtatgt gctcctcact tcatagtgaa acagctcgct
      841 gcgcgcatcg cccatcaggc gaagcagttc cggcactccg ctcagcagcg ctccctgccg
      901 gtccagctcc tgaatccggg cggccaactc gcgcagcaac tcggccagac ggtccgcccg
      961 gtgcttgtcg tacacttcat catgctcagc catgtatccc tccgggtcct ctcaggaacc
     1021 cgggtgaagt tttcccggtt atcaggcgat aaatgtaacg cggacgccaa agggcgagca
     1081 ttgggatcct ttgctccatg cagcgcggcg tagttatctt tcgcgcggac gcgatgaagc
     1141 gtccgaaccc tgtgaacctc aaggaaggta gatgagcaag aagaagccca gacgtttcgt
     1201 ggcaaccgcg acctcgttcg atcaggcccg tgacgagctg ttcagtcaca ttctgcgctg
     1261 tggcgtgatc gatgctcttc ccgagcacca gaaggaatgg ttcgacgata cgatgcagta
     1321 tctcgccgac cggtactcgg atttgagcga cgaagatctc ggcaggctcc gcacgctggg
     1381 cgaacgctac tgccagcccg tgcaacgtcg gagtgagacc gcatcgcggg agaacgccgg
     1441 ctcgttggct ggctgaccgc cgaattcgcc gtcaaccacc gccccaggac ccgccggtcc
     1501 cggggcggtg tcacttcggg gcccgtcgcc accgccaacg ccacccagcc gtaaatcgaa
     1561 agccacctcc cacaccagcc tcgacagccc atcccgatcc gcggccgggg gcgctctcca
     1621 ttccgacgag cgccacaacg tacccctgat cgaccgggcc caccacgccg gcaattcctc
     1681 cgcccacata cacccccggc cgagtcgtcg cccccggact cagcaggaaa tgcccgagca
     1741 actcaccccg gcctgcagct tggccatcgg aaactccgcc gcccaggctc agcgcgatgc
     1801 gggcccgtgc cgcaacccgc atggcaccgt agacgccacc ggttacaaga gcagggtcgg
     1861 agctcgtaac cagagcctga acgccgatct ctcgtgcttc ttgggctgcc aggaggccgg
     1921 gaatgacagc cgcggctgcc aatacccagc taggccaggt cgagctcata ctcgaccgcg
     1981 atgtcaggcg cgagcgagtg caccaccgag cagtatttct gtagcgacag ctccacggcg
     2041 cgccgcacct tggcttgttc gacggcgccg cctttgacat ggaaggtgaa tcgaagcgac
     2101 cggagtcgct ttgggtcctg cgcctgccgg cggccttcca cctcgattcg gagtgctgca
     2161 agcccaaccc gcatcttccc caggatgctc acgacgtctg cccctgagca ggccgccgcg
     2221 gaaagaagaa gtgtcagcat gggtccgggc gcggttgcat tgtcggcatc gatcaacgtc
     2281 gtcggtccgc cgggctcacc tccacgaaag cgcatgtcgc cttcccaggt gagttccacc
     2341 cgccgcacac tgccttccat tgacaggtta ccggctgccg accggccgct tttcgctgcc
     2401 ggccactcgg ctgtagtccc aaatggcata caggccgacg gccccggttg cccaagcgag
     2461 cggtcgcagg aaggaagagt cgagcaggaa ggccaggagc gtgagacact gaccgacggt
     2521 caccgcttta cctcccaacc gggctggtat tgcgctgggc cggcgggaga cggcggtgac
     2581 tacgaaggca accgtcgcga cgatgtcccg caccaacacg cctgcgacct cgtaccactc
     2641 gaggcgcccc gagaatgcaa caaccccgaa ggcgcaggcc atgaacagct tgtcggcgat
     2701 cggatctagg accgccccga acgcggatga tccgaatcgc cgcgcgatgg gcccgtccag
     2761 aagatccgtt gcggctgcga cggcgacgat cgtgaccctc gcccaaacgt cttcggccag
     2821 gagaaacagc acagccagcg gcaggcgaac ggcagtgaga acatctgcgg ctgtccagcg
     2881 gcgtgacaca cgtgacgtgc gaggccggct cacccccgcg gcgggatcgg catcggggac
     2941 gctcataggc ccaaacatac atcgccggga gcacgtgacc gattttaccg gcctccgccg
     3001 ctgccacgca cgactccctc cgcgcgtatg aggcccttga aagtctgctc agccagacgc
     3061 tgaatctgag atgcagtgac gctgcgatag gcggctgcct gattttccag ttcttccaga
     3121 ccgcaaccga ccagccacgc ttcgagcaga tcacctgcga ccgcggtggc gtgctgtctc
     3181 cgcacctccg cctgtccggc caggtagttg atcgcctgtg ccagctcatc ggcggtcacc
     3241 ggcccagcgg cgaatcgctc caattcggcc agcatggctg accgcgcctc ggcctcgcgt
     3301 tcgggcgagg tggcgatgta ggtcacgaga gcgccggccc gtcccttctg ccacgacgat
     3361 gccattacag agtacgccag ggagcgccgc tcgcgtagcg cctcgaagat ccgacccccc
     3421 agtccgctgg caacggcagc ccagacctcg gctgcgtgcc ggtctgcgct gcggcgcgac
     3481 ggaccacgga acaccatcgc cagggcgctc tgagccttgt cccgctccac caccc
//
LOCUS       DASRJQ010000035         2000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2227564, whole genome shotgun sequence.
ACCESSION   DASRJQ010000035 DASRJQ010000000
VERSION     DASRJQ010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2227564"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..882
                     /gene="deoC"
                     /locus_tag="VFB89_00830"
     CDS             130..882
                     /gene="deoC"
                     /locus_tag="VFB89_00830"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HZM25880.1"
                     /translation="MSQGLPPWLDAGAGGRPGIAHLLDYTLLGPEATRDDILRLCDDA
                     AAAQIWAVCVNGGWVSTCAARLRQTGIRLAAVVGFPFGAASGAAKAAEAAIALADGAT
                     ELDMVFQLGRAKAGDWATVTEDVALVVAAARGALVKVIIESAALSADEVSRACAAAMI
                     GGAGFVKTSTGFHPSGGATIDAVRRMRDAVGLVLGVKASGGIRTQEQALSMIAAGANR
                     IGLSSLAGLASIIGPKAPSLNQLLSRQASGVT"
     gene            879..1787
                     /gene="dat"
                     /locus_tag="VFB89_00835"
     CDS             879..1787
                     /gene="dat"
                     /locus_tag="VFB89_00835"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR01121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047810 - D-alanine:2-oxoglutarate
                     aminotransferase activity [Evidence IEA];
                     GO_process: GO:0019480 - L-alanine oxidation to pyruvate
                     via D-alanine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HZM25881.1"
                     /translation="MNDLVFLNGNVLPRSRALVSIDDRGFLFGDAVYEVIRSLPGRFI
                     QPERHLNRLARSLREISIPAPDLDLLAIATDLLGRNGLDQREALIYLQVSRGAAQRQH
                     AFPPDSVQPTVLMTVAALEPRREQARRGVAAITGPDLRWSRCDIKSVNLLANVLAAQH
                     ASEAGAFEAILIRDERVTEATRSNVFAVIDGTLRTHPTGPFILPGVTREVVMELCGKA
                     ALRVREEAITRNELARAEELFVTGTTADVMPVVTLDGRPVGSGRPGPVTRRLSALLAS
                     EMGLDGVGAGSRHFDGDATAGLDSVQ"
     gene            complement(1716..>2000)
                     /locus_tag="VFB89_00840"
     CDS             complement(1716..>2000)
                     /locus_tag="VFB89_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25882.1"
                     /translation="AAVGALAVPVDHAPPGGPVRVVIRSYDLKFWRSPNGVATVQRVQ
                     TLGDRVKVEATIDGGSPLFAHFPRRSSLLHGIEPGCRISVEVTRARAYPI"
BASE COUNT          336 a          608 c          699 g          357 t
ORIGIN      
        1 agaaggtggc gtcgcgttct gggcccacgt gggtggattt ctcggaggcg cgctgctggt
       61 tccgttgttc cgaaatccgg tgttcgtggc cgaacatcga cgactcgccc gcacctacgc
      121 cggcgcctgg tgagccaagg acttcctccc tggttggacg ccggggccgg gggtcggccc
      181 ggtatcgcac atctgctcga ctacacgctg ctcggccctg aggcaacccg agacgacatc
      241 ctgcggctct gtgacgacgc ggccgcggct cagatctggg cggtctgcgt caacggcggc
      301 tgggtcagca cctgcgccgc tcgcctgcgg cagaccggaa tccgtttggc tgcggttgtc
      361 ggatttcctt ttggcgcggc aagcggagcc gcaaaagcag ccgaagccgc aatcgcgctg
      421 gccgacgggg caacggagct ggatatggtg ttccagctgg gccgcgccaa ggctggagat
      481 tgggctacgg taacggagga tgtcgcactg gtggtcgctg ctgcgcgggg cgccctagtc
      541 aaagtcatca tcgagagcgc ggccctgagt gccgacgaag tgagccgagc ctgcgctgcg
      601 gcgatgatcg gaggagccgg gttcgtcaag acgtctaccg gctttcatcc atccggtggc
      661 gcgacgattg atgccgtgcg ccgcatgcgg gacgccgttg ggctggtgtt aggcgtgaag
      721 gcctcgggcg gaattcggac tcaagagcag gcactgtcca tgattgcggc cggcgcaaac
      781 cgcattggac tgtcgagcct cgccggtttg gcgagcatca tcggaccgaa agccccctca
      841 ctcaaccagt tgttgagccg acaagcaagc ggggtaacat gaacgatctg gttttcctga
      901 acggcaacgt gctgccgcgg tcgcgagcct tggtgtcgat cgacgaccgc ggctttctgt
      961 ttggtgatgc ggtctacgag gtgattagaa gcctgccggg gcgattcatc cagccggagc
     1021 ggcatctcaa tcggctcgca cgcagcctgc gtgagatttc gatccccgcg cccgacctcg
     1081 acctgctcgc catcgcaaca gacttgctcg gcaggaacgg attggatcag cgagaggcgc
     1141 tcatctacct gcaggtgagt cgcggagcgg cccagcgcca gcacgcgttt ccacccgatt
     1201 cggtgcaacc cacggttctg atgaccgtcg cggcgttaga gccccggcgg gagcaggcaa
     1261 gacggggtgt cgccgcgatc accggacccg acctgcggtg gtcgcggtgc gacatcaaat
     1321 cggtcaatct gctggcgaac gtgctggccg ctcagcacgc gagcgaggca ggggctttcg
     1381 aggcgatcct gattcgcgac gagcgagtga ccgaagcaac gcgcagcaat gtgtttgccg
     1441 tgatcgatgg aactctgcgc actcatccga ccgggccatt cattttgccg ggtgtgaccc
     1501 gcgaggtggt gatggagcta tgcgggaagg cggcgctgcg ggtccgtgag gaggcgatta
     1561 cgcgtaacga gctggcccgg gcagaggagc ttttcgtgac cggcacgacg gctgacgtaa
     1621 tgcccgtggt tacgctggat ggtcggccgg tgggaagcgg caggcccggt cccgtaaccc
     1681 ggaggctctc cgctctgctg gcaagcgaaa tgggactaga tggggtaggc gcgggctcgc
     1741 gtcacttcga cggagatgcg acagccgggc tcgattccgt gcagtaggct gcttcggcgg
     1801 ggaaagtggg cgaacagcgg gcttcctccg tcgatggtcg cctcgacctt cactcgatct
     1861 cccagggtct gcactcgctg caccgtcgcc acgccattgg gcgatcgcca gaacttgaga
     1921 tcataggacc ggatgaccac ccgaacgggt cctccggggg gcgcgtgatc caccgggaca
     1981 gccagcgccc ccactgcggc
//
LOCUS       DASRJQ010000036         6348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_817602, whole genome shotgun sequence.
ACCESSION   DASRJQ010000036 DASRJQ010000000
VERSION     DASRJQ010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6348
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817602"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..2300
                     /locus_tag="VFB89_00845"
     CDS             168..2300
                     /locus_tag="VFB89_00845"
                     /inference="COORDINATES: protein motif:HMM:NF039722.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6311 domain-containing protein"
                     /protein_id="HZM25883.1"
                     /translation="MISEPRRPVGARVLLAAAALGALWFCVVYPLRILNPSNFDWLLS
                     ADRAEALVGWLFFRNEPWRLPLGSITSMVWPLQTTVALSDSIPWLATFFKLVLPAMQG
                     QFQFFGIWLLGCFMAQAVFGLLLLHAWTDRVDLSLLGTSFLLLSPPLLGRYPHLSLCA
                     HWQILAALWLYCRTTPRTAQGRIAAGWAGLTVIAASTHPYLAAMVLMIGAAAHVRYWL
                     WDRSLSRGHALARMVGMAALCVGTWAVWGYWSSGASPSATGFGRYTADLLTFIDPRYY
                     SAFLPALPTSEQQWEGNSYLGLGLMLLAVVAVLPLARLRIRRDMVLRYLPLITVTSAM
                     LIFAIGTPVTLAGRPLFHVHGWGLLDPLPAAFRSSGRFIWPAYYLLVAIVFAGVALLP
                     SARVAMAVTAVALGVQVLDLRPLLHRLHEESDRPRTDSLISPSFHDSRPFFDAVQLVP
                     RVAVDGPMPFCYRGWGPANALIPFSLFAARERLRFNSAKLARWPEQQVAAACRAAMDS
                     LKAGILNERTIYIVGEPFAGIFAWWIGDKAVCNRLDGHIVCVARSNPSPLRAALSGPI
                     QGAPIDRSVELDFTNRTKDNPAVLSFMGWTRPRPNGREMADTLADIRLAAPLAGTVIL
                     EITASPTGSASDCTLLVSVGGTSRRLALAVRPSINVLRFDDIVATDTVRLSLPSGERS
                     CRSVNGAPAVRLTHMLIRLQRRPDTHPL"
     gene            complement(2413..3963)
                     /locus_tag="VFB89_00850"
     CDS             complement(2413..3963)
                     /locus_tag="VFB89_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25884.1"
                     /translation="MLLSIGVFLRLWQYLDRTSLFVDEIALAQNLLVRSPGALLTEPL
                     AFNQLAPKAFLLVEKFIAWGFGAGELQLRLLPFLAGLVSLPLFTLLALKLELRAGALF
                     GVALFSLAVPLLRLGAQVKPYTLDVFATLALLLVSLRWRERHGRTEAVALAGLGLISV
                     WFSFASVLVLFSLGVTLAGYAFRDRDPHEVRLIAIVLLTWLVAASAGVTVARSSETPA
                     MHGFLTSTWASALMPPPWHLPEMLHWFSGTYKDLMRRTLGYPWRAMYLALMVVGAIVL
                     WRRRSDAVAILLAPAAGALVAAAVHQYPFRDRLVVFILPSLFLLMGEAVDYMWRRLSS
                     VPRLFRVGIGVALLGGPIYALIQHPPVYRPDDMRTVLSHIRAGSGPGKVAYIYYGAGP
                     GTTYYGAEFGLEALRLVQGKCHHEQPRLYLTELDQLRGQSQAWIIFARAAAGERELLL
                     SYLDRIGTRLDSVGVVTKGGPEVTWASAYLYDLSDPYRLAMASAATFPLPVLPRIPDV
                     WQPCQAGG"
     gene            complement(4081..4542)
                     /locus_tag="VFB89_00855"
     CDS             complement(4081..4542)
                     /locus_tag="VFB89_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25885.1"
                     /translation="MAHLDDRTATRESEGRIRTEYPFEAFLEDLDALNANDSLPRAPF
                     HRIPMAALPHFRAKYLKARIRHFRFTRFMLAMSYIAEHFHRFESEDLAVYGSDRVGGL
                     ISEALLRAVHQKFSVPDVFAMEGSPDPDAVVALARELRTALPRQGRRALGV"
     gene            complement(4582..5061)
                     /locus_tag="VFB89_00860"
     CDS             complement(4582..5061)
                     /locus_tag="VFB89_00860"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HZM25886.1"
                     /translation="MAFVEALIQLAAATVAGAVIGLNRDLRGKPAGVRTHALVGLGSA
                     VATLVASSAFVNTPDPAAASRVIQGIITGVGFLGAGVILHPDGGRSVRGLTTAASIWV
                     VAALGVACGTAQWPAAIVGTGLTLTVLLLGGPLERLAHRLRRGREDDSTLTPRPPSA"
     gene            complement(5087..>6348)
                     /locus_tag="VFB89_00865"
     CDS             complement(5087..>6348)
                     /locus_tag="VFB89_00865"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZM25887.1"
                     /translation="GSNNLEVYADLRPRGEWGPIKDKNELVTLMSRQLSGIPGLGLNF
                     SQYIKDNVEEALSGVTGELVVKIFGPDMTVLQEKAEEVERVLSRIRGVADLGVEEQFG
                     QPQIRFEAERMELARYGLNVADLNDAIETAVGGRAVTRLLEGDRVFDVRVRFAAPARS
                     SRTALEELPVSATDGPGVPLASVAKVVSAEGASRIAREANQRRIAIKCSVRGRDQGSF
                     VAEGQRLVAQRVLLPPGYRITWGGQFENQRRAAARLEVIVPASIVLIFVLLFSAFGSV
                     RYATLILANLPFALIGGILVLWIRDINLSVSAAVGFIALFGISVQNGVVLVTEFNRLR
                     EQGLPIHEAVRRGCMDRLRPVVMTALMAALGLLPAAFSRGIGAETTRPFASVIVGGLV
                     SATVLTLLLLPILYLAFHEEPQEQVQH"
BASE COUNT         1192 a         2039 c         1984 g         1133 t
ORIGIN      
        1 gaccctctgc ttagaaggca gatgctctat ccagctgagc tacgggcgca aagtggtgac
       61 gcgataagaa gctagcgccg cgcgtcccgg agtgggagcg acgagggtgc gtgagaactt
      121 gagcggatcg cgactcgcgc ctgccgctcc aggaggtcgg tcgcgtaatg atttctgagc
      181 cacggcgtcc ggtaggcgct cgcgtactgc tcgcagctgc cgccctcggg gccctttggt
      241 tctgcgtagt ttacccgctt cggattctga acccatccaa ttttgactgg ctgctatctg
      301 ccgatcgggc cgaggctctc gtcgggtggc tcttctttcg aaacgagccg tggcgcctgc
      361 cgctgggctc catcacatct atggtgtggc cgctgcagac gacagtcgca ttgtccgact
      421 cgatcccgtg gttggcgaca ttcttcaagc ttgttcttcc agcaatgcag gggcagttcc
      481 agttcttcgg catttggctg ctgggatgct tcatggccca ggccgtcttc ggattgctcc
      541 tcctacatgc gtggacagac cgggttgacc tgagtctcct ggggacttcc ttccttctac
      601 tgagcccccc tttgctgggc cgctacccgc acctgtcgct ttgcgcgcat tggcagattc
      661 tggccgcgct atggctttat tgtcgcacga ccccgcgcac cgcacagggc cggattgcgg
      721 ccggttgggc aggcctcacg gtaattgccg cttcaacaca cccatacctg gcagccatgg
      781 tcctcatgat tggagcagct gcccacgtga ggtattggtt gtgggacagg agtctctcga
      841 gagggcatgc cctggcccgg atggtcggca tggccgcgct ctgcgtgggc acatgggcag
      901 tttggggata ctggtcgtct ggagcgagcc cctccgcgac gggcttcggc cgttacactg
      961 cggaccttct cactttcatt gacccgagat actactccgc attcttgcct gccttgccca
     1021 ccagcgagca acaatgggaa ggcaattcgt acttggggtt gggcctgatg ctgttggctg
     1081 tcgtggcggt gctcccgctc gcacgacttc gcatacgacg agacatggtg ttgcggtatc
     1141 tgcccttgat cactgtgaca agcgcgatgc tcatctttgc gatcggcaca ccagtgacgc
     1201 tggcagggcg gccgctgttc catgtacacg gttgggggct gctcgacccc ctgcccgcag
     1261 cgttccggag ctccggacgc ttcatatggc ccgcgtacta cctcctcgtt gcgattgtct
     1321 tcgcaggtgt ggcgttgcta ccctccgccc gcgtagcaat ggcggtgact gcagttgcgc
     1381 tgggagttca agtgctcgac ctgcgcccgc tcttacatag gctgcatgaa gagtccgacc
     1441 gacctcgtac ggattcgctg atttcaccaa gcttccatga tagccgccca ttcttcgatg
     1501 ctgtgcaact ggtcccccgg gtcgccgtcg acgggccgat gcctttctgt taccgcggct
     1561 ggggtcctgc caacgcgctc attccgttct ctctttttgc ggcccgggaa cggcttcggt
     1621 tcaacagtgc caagctcgct cgttggcccg agcagcaggt cgccgctgcg tgcagggcgg
     1681 cgatggacag tctcaaagct gggatactga atgagcgcac gatctacatc gtgggcgaac
     1741 cctttgcagg gatcttcgca tggtggatag gcgacaaagc cgtctgcaac cgcctcgacg
     1801 gtcacatcgt ttgtgtcgct cgaagcaatc cgtccccgct cagagcggca cttagcggtc
     1861 ctatccaggg tgcacctatc gatcggagtg tggagctgga tttcaccaat cggacgaagg
     1921 acaacccggc cgttctttcc tttatgggct ggacccggcc tcgaccgaat ggtcgggaga
     1981 tggcggacac ccttgcagat attcgcttgg ctgctcctct tgccggcact gtgatcctcg
     2041 agattaccgc ctctccgacg ggatcggcta gcgactgcac tctgctggtc tccgttggcg
     2101 gcacctcgcg caggctggcg ctggccgtgc ggccaagtat caacgtgcta aggttcgacg
     2161 atatcgtagc cacggacaca gtgcggctca gccttccttc cggggaacga tcttgccgca
     2221 gcgtgaacgg cgcacccgcc gtacgactga cgcacatgct gattcggcta cagcgccgcc
     2281 cggacaccca tccactgtga gttctatcaa gctgagctac gggcgcaatg tggtgatgga
     2341 tcagaaggtc gtccctcact ccactgatga gggggaggaa taggaggcgc cgccccgcag
     2401 actggttgag gcttaaccgc ctgcctggca gggctgccac acatcgggaa tgcgcgggag
     2461 aaccggcagc gggaatgtgg ctgcggaggc catggctagc cggtatggat cgctcaggtc
     2521 ataaaggtag gcacttgccc atgtcacctc cggcccacct ttcgtcacca cgcctacgct
     2581 gtcgagccgc gtaccaatcc tgtccagata actcaggagc agctcacgct caccggcagc
     2641 agcgcgggcg aagataatcc aggcctgcga ctgtccacgc agctgatcga gctctgtcag
     2701 atagagtctc ggctgttcgt ggtggcattt gccctggacg agtcgcagtg cctcgagacc
     2761 aaactctgcc ccgtagtacg tcgtgcctgg tcccgcgcca tagtagatgt atgcaacttt
     2821 tccgggcccc gagccagcgc ggatgtgcga gagtacagtc cgcatgtcat cggggcgata
     2881 gaccggtggg tgctgaatga gcgcgtagat cgggccgccc aacaacgcga ccccgattcc
     2941 gacgcgaaac aatcgcggga cggagctgag tcgacgccac atatagtcga ccgcttcacc
     3001 catcaacaag aacagcgatg gcagaatgaa caccaccagt cgatcccgga agggatactg
     3061 gtgcaccgcc gctgccacga gcgccccggc agcaggggca agcaggatgg caacagcatc
     3121 cgatcgacga cgccagagta caatcgcgcc gacgaccatg agcgcgaggt acatcgcccg
     3181 ccacggatat ccgagcgttc gtctcatgag atctttgtat gtgcccgaga accagtggag
     3241 catctccggc aggtgccaag gcgggggcat caaggccgat gcccaggtag aagtgagaaa
     3301 gccatgcatc gctggtgttt cactggagcg ggcgacagtc accccggcgg aagccgcgac
     3361 cagccacgtc aacagcacga tcgcgatgag tctcacctcg tgcggatcgc ggtcgcggaa
     3421 cgcgtagccc gcgagagtca caccgaggct gaacagtacc agaacggacg cgaacgagaa
     3481 ccaaacggag atcagaccga gccccgcaag cgccaccgcc tcggtacggc catgtcgctc
     3541 acgccaacgg agcgacacca acaggagagc caatgtggca aatacgtcga gcgtataggg
     3601 cttcacttgt gcgccgagtc gcagaagcgg cacggccagg ctgaatagtg ccacaccgaa
     3661 gagcgcaccc gctcgaagct ctaacttgag agcgaggagc gtgaagagcg gcagggaaac
     3721 gagccccgcg agaaatggca ggagtcggag ctgcaattca ccggccccga agccccaggc
     3781 gatgaacttc tccaccagaa ggaacgcctt gggcgcgagc tgattgaacg cgagcggttc
     3841 ggtgagaagg gcgcccggcg aacgcaccag gaggttctgg gcaagcgcga tctcgtcgac
     3901 aaacaaggag gtgcggtcga gatactgcca gagacggaga aacactccaa tcgacaggag
     3961 cacgacgaga gcagcccaaa tccagccgcc ggccgaagcc agctcggaat tgggcccact
     4021 acctgagtta tttgcgcggt cgccggggga aatgagaggc tcgagtgggc tgacctgaag
     4081 ctacacgccg agcgcccgcc gcccctgcct gggcaaggcg gttcggagct cccgcgcgag
     4141 tgcgaccacg gcgtccgggt cgggacttcc ctccatggca aagacgtcgg gcaccgagaa
     4201 tttttgatgc actgcccgga gcaaggcttc gctgattagc ccgccgaccc gatccgaccc
     4261 gtacacggcc agatcctcgg actcgaatcg atggaaatgc tcggcgatgt aactcatcgc
     4321 cagcatgaac cgggtgaacc ggaaatggcg gatacgcgcc tttaggtact tagctctgaa
     4381 gtgtgggagc gcggccatcg ggattcgatg aaacggggcg cgcggaagcg agtcgttcgc
     4441 gttcagggcg tcgaggtcct ccagaaacgc ctcgaacggg tactccgttc gtatgcggcc
     4501 ctccgattcc cttgtcgcgg tgcgatcgtc gaggtgtgcc acgctcgcgg gcttcctttc
     4561 gctgcgatcc tgccggtcag gctaagcgct cggtggtcgc ggggtcaatg tcgagtcgtc
     4621 ttcgcggccc cggcgaagcc gatgggccag ccgctcgagc ggtcccccta acaagagaac
     4681 ggtcagagtc agcccggtgc cgacaatggc agcgggccac tgtgcggtgc cacacgccac
     4741 tcccagggct gcgacgaccc aaatggacgc cgcggtggtg agtccacgga cgctccgacc
     4801 gccatctggg tgaagaatga cacccgcacc gagaaagcca acgccggtaa tgattccctg
     4861 gatcactcga ctggccgcgg ccggatccgg ggtgttgaca aaggccgatg acgctaccag
     4921 cgtcgcgacg gccgacccca ggcctaccag cgcgtgagtc cggacgccgg cgggtttgcc
     4981 ccgcaggtcc cgattcaagc cgatgacagc gccggcgacc gttgccgcgg ccagctggat
     5041 gagcgcttca acgaaggcca tggcgagaat ctagcgcgcc ggagcctcag tgctgcacct
     5101 gctcctgtgg ctcctcatgg aacgccaggt agagaatcgg cagcagaagc agcgtgagca
     5161 cggtcgccga aaccaggcca cccacgatga ccgacgcaaa cggcctcgtc gtctcggccc
     5221 cgattccgcg agagaacgcc gccggcagca ggcccagggc ggccatgagc gcggtcatca
     5281 ccaccggccg aagccggtcc atgcacccgc gccgaaccgc ttcgtggatc ggtaggccct
     5341 gctcccgcag gcggttgaat tcggtgacga gaaccactcc attctgtacc gagatcccaa
     5401 acagcgcgat gaagcccacc gccgcagaca cgctcaggtt gatgtcccgg atccagagga
     5461 ccaggatgcc gccgatcagc gcaaagggaa gattcgcgag aatgagggtc gcgtatcgaa
     5521 ccgacccgaa cgcgctgaag agaagcacga agattagtac gatgctcgcg ggcacgatta
     5581 cctcgagccg cgccgccgcc cgccgctgat tctcgaactg accaccccag gttatccgat
     5641 agccgggcgg cagcaagacc cgttgagcaa ccaggcgctg cccctcggcc acgaagcttc
     5701 cctggtcccg cccgcgcacc gagcacttga tcgcgattcg tcgctggttc gcctcgcggg
     5761 cgatgcggga ggcgccttcg gccgacacga cctttgccac gctcgccaga gggaccccgg
     5821 ggccgtctgt cgcgctgacg ggcagctctt ccagcgcggt gcggctcgag cgggccggcg
     5881 cggcgaagcg gacccggacg tcgaacaccc ggtcgccctc cagcaatcga gtgacggccc
     5941 ggcccccgac cgcggtctcg attgcgtcgt tgaggtcggc gacgttgagg ccatagcgtg
     6001 cgagctccat gcgctccgcc tcgaagcgaa tctgcggctg cccgaactgc tcctcgacgc
     6061 cgagatccgc cacgccacgg atccggctca acacccgctc gacctcctcg gccttctcct
     6121 gcagcaccgt catgtccggg ccgaagatct tcaccaccag ctcgccggtg acgcctgaga
     6181 gagcctcctc gacgttgtcc ttgatgtact gggagaagtt gagcccgagc ccgggaatac
     6241 cggagagctg ccgagacatc agcgtcacca gctcgttctt gtcctttatc gggccccact
     6301 ccccgcgcgg gcgcaggtcc gcgtatacct cgaggttgtt ggagcctt
//
LOCUS       DASRJQ010000037         5321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3922057, whole genome shotgun sequence.
ACCESSION   DASRJQ010000037 DASRJQ010000000
VERSION     DASRJQ010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5321
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3922057"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1304
                     /locus_tag="VFB89_00870"
     CDS             <1..1304
                     /locus_tag="VFB89_00870"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HZM25888.1"
                     /translation="LLTLAQMQYLRDVTYPGQLAQLKRNIAVYQEMVAQSDEARRRWE
                     NQLFYAQNSYKAINGYEAGLLDTAIMAKKQKFERDFRARINRDPKLRAKYGDAWDRIA
                     AALKEERTLAVKSRFYGFGGSQLLAIAGVLLRLPEQGALPDSARLPAYRGEGLDKAKA
                     LVQGDVPIDKEVEKATLAGQLTAARKELPASDPYLNAILQGRSPEVAAEALIEKTSLA
                     DPAARKALLDGGASAVKASNDPLMVVARKIAPLSRNVAMRMEGLESVASENAERIGQA
                     LYAAYGTSLPPDATFTLRITDGVVQGYPMNGTKASYKSTMYGLYGRSADFDGQPPYQL
                     PERWKAARDRLDLATPMDFVTTNDIIGGNSGSPVINQKAEVVGLIFDGNIESLPNRFI
                     FTDEVARSVAVHSRGITEALRKVYDAGRIADEMEGTKVSQQ"
     gene            complement(1393..1881)
                     /locus_tag="VFB89_00875"
     CDS             complement(1393..1881)
                     /locus_tag="VFB89_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25889.1"
                     /translation="MPRTLRLTSPLLLLLAIIPGGTAWPQARGRGVATLTAGPRAGRD
                     FENHAWSLGGQVGFMLRDRFEIRPSGDYFFGDETPFRWQLNADGALTFGPGRSIYAGG
                     GAAFVKVLPLDDVETGYNIFFGLNLAKPQSRSRPFAEFRWTWVNDTSPFRLALGFNYR
                     LR"
     gene            2181..2381
                     /locus_tag="VFB89_00880"
     CDS             2181..2381
                     /locus_tag="VFB89_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25890.1"
                     /translation="MSRKLSIPTLALLAALVAVPVMAQQPTDSAHKATAATTHHKSKH
                     HKSSTKDTTKAATAKKDTTMKK"
     gene            complement(2479..5232)
                     /locus_tag="VFB89_00885"
     CDS             complement(2479..5232)
                     /locus_tag="VFB89_00885"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZM25891.1"
                     /translation="MYPRLCGRLALASACVAVSGVPVLEAQHAATPAATRDIEIPYQQ
                     FVLKNGLTLLVHEDHKAPIVAVNLWYHVGSKNEKPGRTGFAHLFEHLMFNGSENYKGE
                     YFTPFEQAGATDQNGTTNEDRTNYFENVPTNALDMALWMESDRMGHLLGAIDQAKLDE
                     QRGVVQNEKRQDENEPYGTVWEFLTPRLYPPNHPYSWTTIGSMEDLGAAKLEDVKEWF
                     QSYYGPANAVLVIAGDVDTKVAREKVEHYFGDIPSGPPVGRQDAWVAKRSGSQRGVLQ
                     DRVPQARLYRVWNVPGWATADGDYLKLVARVLSDGKTSRLYKRLVYDDQIATDIAAVV
                     DTREIGGLFYIQATARPGDDLSKVERAVNEEMARFLRTGPTPSELARVKTDFRADFIR
                     GVERIGGFGGKSDVLAQGAVFAGKPAHYKVRLQRVASATPGDLLRVAKQWLGDGDYTL
                     EVKPFPNYQTATTGADRSKLPKPGTPPDAKFPNLERATLSNGLKIVLAERPSVPQVRF
                     DLLLDAGFAADQSAEPGTANMTLAMLDEGTQRRTSLQISDELQRLGATLTAFSRLDYS
                     SVTLDALKENLDPSLALFADVVLNPSFPQKDFDRLKKQQLAAIEREKAEPFTMALRAF
                     PGLVYGTGHAYATPWSGTGTEESAAKITRAQVAAFHHDWFKPNHATLIVVGATTMNEI
                     KPKLERLFAGWKPGDIPAKKVNDVALPATPAVYLIDRPGALQTVILAGNLAPPKNNPN
                     EVRIEVMDAILGGNFGSRINMNLREDKHWSYGAGTFLRDARGQRPFVAYAPVQTDKTK
                     ESIIELSKELQGILAGRPVDSTELAQAKSSLTLTLAGGWETMEEVDATIRSIVTFGLD
                     DRYFDTYPTKVRAVDAGQVPAAAAETVHPDKLVWLIVGDRAKIEAGVRELKLGEVRVI
                     QPT"
BASE COUNT          995 a         1698 c         1658 g          970 t
ORIGIN      
        1 gactgctgac actggcgcag atgcagtatc tgcgtgacgt cacctatccg gggcagctcg
       61 cgcagctcaa gcgaaacatc gcggtctatc aggagatggt ggcgcaaagc gacgaagctc
      121 ggcggcgctg ggagaaccag ctcttctacg cgcagaattc ctacaaggcg atcaatggct
      181 atgaggccgg gctgctcgac accgcgatca tggcaaagaa gcagaagttc gagcgggact
      241 tccgggcccg aatcaatcgc gacccgaagc tccgggccaa gtacggcgac gcctgggacc
      301 ggatcgcggc cgcgctcaag gaagagcgca cgctcgccgt gaagtcccgc ttctatggct
      361 tcggcggatc acagctgctt gcaatcgccg gcgtactgct ccgcctgccc gagcagggcg
      421 cgctcccgga ctccgctcga ctgcccgcct accggggcga aggcctcgac aaggccaaag
      481 ctttggtgca gggcgacgtg ccgatcgaca aggaagtcga gaaggcgacc ctcgctggtc
      541 agctgactgc ggcgcgtaaa gagctgccgg cctccgatcc atatctcaat gccattctcc
      601 agggccgaag cccggaagtc gctgccgaag cgctgatcga aaagacgtca ctggccgacc
      661 ctgcggcgcg gaaagcgttg ctcgacggcg gcgcgtcggc ggtgaaggcc tccaacgatc
      721 ctctaatggt ggtggcacga aagattgcgc cgctctcgcg aaacgtcgcc atgcggatgg
      781 aggggctcga gtccgtcgct tccgagaacg ccgagcggat cgggcaagcc ctgtacgcgg
      841 cgtacggcac gtcgctgccg cccgatgcaa cgttcacgct gcggatcacg gacggtgtgg
      901 tgcagggata cccgatgaat ggaaccaagg cgtcatacaa gagcacgatg tacggccttt
      961 acggacgctc ggccgacttc gacggccagc cgccgtatca gctgccggag cgttggaaag
     1021 ccgcccggga tcggctcgat ctcgcgactc cgatggactt cgtgacgacc aacgatatca
     1081 tcggcggcaa ctcgggcagc ccggtgatca atcagaaggc ggaagtggta gggctcatct
     1141 tcgacgggaa catcgaatcg ctgcctaacc ggttcatctt cacagatgaa gtggctcgct
     1201 cggtggcggt ccactctcgt ggaatcaccg aggcgctccg gaaggtgtat gatgcaggtc
     1261 ggatcgcgga cgagatggag ggaacgaagg taagccagca atagcgggag aaagagagaa
     1321 aaggggaaag ggggacaggg agaaaggcgt aggcttgcct gccctttccc tttctcccat
     1381 tcagcgccgc attcaccgca gtctgtagtt gaatcctaac gccaaacgga acggactcgt
     1441 gtcgttcacc caggtccagc gaaactccgc gaagggccgg cttcgggact gtggcttcgc
     1501 gaggttaagc ccgaagaaaa tgttgtaacc ggtctcgaca tcgtcgagcg gcagcacctt
     1561 cacgaaggct gctccccctc ccgcataaat gctccgcccg ggcccgaagg tgagcgctcc
     1621 gtcggcgttc aattgccagc gaaatggggt ctcatcgccg aagaagtagt ctccgctcgg
     1681 acggatctcg aaacggtccc gcagcatgaa tccgacctgc ccacccagac tccaggcgtg
     1741 gttctcgaag tcacgcccag ctcgcgggcc ggcggtcaac gtagccacgc ctcttccccg
     1801 cgcctgaggc catgctgtgc cgccagggat gatcgcgagg agaagaagca ggggcgatgt
     1861 cagccggaga gtgcgaggca tcgaacctct ttgaccagaa tgctatgggc gcgcgagcgc
     1921 cggatgttaa tggagacggg acattttgtc actgcgccga cccgtctgtc cctgtgagcc
     1981 gggtctggtt ggcgcgtggc ctcgcgaagc cggtcgagtg attgcgtgcc aaaatcattt
     2041 ctgcaaagca catgcgcgcg agcactccga ttggcgcgac tcctgccttt gcctccaccg
     2101 cacgccggtg gcccagcacg ggagaagccg gcgacatgac cttcattgtc tcacgtcagc
     2161 gttaccccac ggaggaacgc atgtcccgga agctctcgat ccccacgctc gccctgctgg
     2221 ccgccctggt cgcggtcccc gtcatggcac agcagcctac tgacagcgcc cacaaggcga
     2281 cggccgccac cacgcaccac aagtcgaagc atcacaagag cagcacgaag gacaccacca
     2341 aggctgcgac ggccaagaag gacaccacga tgaagaagta ggcggaacag ccagtaaagc
     2401 gtgaacagta aacagtgaac gggggctccg atcagcggaa ccccccgttc actgtttacc
     2461 gttcaccgtt cacgcagttc atgtgggttg tatgaccctc acctctccca gtttcaactc
     2521 ccgcacgccg gcctcgatct tggcccgatc accgacgatc agccatacca gcttgtcggg
     2581 gtgtaccgtc tccgccgcgg cggcgggcac ctgacccgca tcgaccgcgc gcaccttggt
     2641 cgggtaggta tcaaagtacc ggtcgtccag gccaaaggtg acgatgctcc ggatcgtggc
     2701 gtccacttcc tccatggtct cccagccgcc tgccagcgtg agcgtaagac tcgacttggc
     2761 ctgtgccagc tcggtcgaat ccaccggacg tcccgcgaga attccctgta gttccttgga
     2821 gagctcgatg atggattctt tggtcttgtc ggtttgtacc ggcgcgtacg ccacgaacgg
     2881 gcgctgcccc cgcgcgtcgc ggaggaaagt gccggcgccg taggaccagt gcttgtcctc
     2941 ccgcaggttc atgttgatcc gcgagccgaa attgcccccg agtatggcat ccatgacctc
     3001 gatccggact tcgttgggat tgttcttggg tggcgccagg ttgcccgcga gaattacggt
     3061 ctgtaacgct cccgggcgat cgatgagata caccgcgggc gtggccggta gcgcgacatc
     3121 gttcaccttc ttcgccggga tgtctcccgg cttccaaccc gcgaagagac gctcgagttt
     3181 cggcttgatc tcgttcatcg tggtcgcgcc taccacgatc aacgtcgcat ggttgggctt
     3241 gaaccagtcg tgatgaaagg cggcaacttg agcgcgcgtg attttcgccg ccgactcttc
     3301 cgtgccggtg ccgctccacg gcgtcgcata cgcgtggccg gtcccgtaca ccagcccggg
     3361 gaatgcccgg agcgccatgg tgaacggctc agccttctcc cgctcgatgg ccgccagctg
     3421 ctgcttcttc agccggtcga agtccttctg cggaaacgac ggattgagca cgacatcggc
     3481 gaatagcgcc agcgacggat cgagattttc cttgagggca tcgagcgtga cgctggaata
     3541 gtcaagccgg gaaaacgctg tgagggtcgc gccgagccgc tgcagctcgt cgctgatctg
     3601 gagcgaggtg cgccgctggg tcccttcgtc cagcatcgcg agcgtcatgt tggcggtgcc
     3661 gggctccgcc gactgatccg cagcaaaccc cgcgtcgagc aacagatcga atcgtacctg
     3721 cgggaccgag ggccgctccg cgaggacgat cttgagtccg ttggacaggg ttgcccgttc
     3781 caggttggga aacttggcgt cgggcggcgt gcccggcttc ggcagcttgg aacgatccgc
     3841 gcccgtcgtt gccgtctggt agttggggaa cggtttcacc tcgagggtgt agtcgccgtc
     3901 acccaaccat tgcttcgcga cccgcaggag atcgcctggc gtcgcagacg ctacccgctg
     3961 aagccgcacc ttgtaatggg cgggcttgcc ggcgaacacg gctccctgcg cgaggacgtc
     4021 tgattttcct ccaaagccgc cgattcgctc aaccccccgg atgaaatcgg cccggaaatc
     4081 agtcttgacc cgcgccagct cgctcggcgt tggtccggtc cggaggaacc gggccatctc
     4141 ctcgttgacg gcacgctcga ccttgctgag atcgtcgccg gggcgtgccg ttgcctggat
     4201 atagaacagg cctccgatct cgcgagtatc gaccacggcc gcgatgtcgg ttgcgatttg
     4261 atcgtcatag accagacgct tgtagagcct cgacgttttt ccatccgaca gcacccgagc
     4321 gaccagcttc aggtaatcac cgtcagccgt ggcccagccg gggacgttcc acacccgata
     4381 gagtcgggct tgcggcacac ggtcctgcaa cacgccgcgc tggcttccac tacgcttggc
     4441 aacccaggcg tcctgacggc cgaccggagg tcccgaagga atgtcgccga aataatgctc
     4501 gactttttcc cgcgcgacct tggtatccac gtcgccggcg atcaccagca ctgcgttggc
     4561 cggtccgtaa tagctctgga accactcttt cacgtcctcc agcttggccg cgccgagatc
     4621 ttccatcgaa ccgatcgtgg tccacgaata cggatggttg ggcgggtaga gccgcggcgt
     4681 caaaaactcc cagacggtcc cgtacggctc gttctcgtcc tgccgcttct cgttttgcac
     4741 cacgccgcgc tgctcgtcca gcttggcctg atcgatggcg ccgagcaggt gtcccatccg
     4801 gtccgattcc atccacagcg ccatgtccag cgcgttcgtg gggacgttct cgaagtaatt
     4861 ggtgcgatcc tcgttggtgg ttccgttctg atcggtagcg cccgcctgct cgaacggcgt
     4921 gaagtactcc cccttgtagt tctcgctgcc gttgaacatc aagtgctcga acaagtgggc
     4981 gaacccggtc ctgcccggct tctcgttctt cgagccaaca tggtaccaga ggttcaccgc
     5041 cacgatcggt gccttgtgat cctcatggac gagcaaggta agaccgttct tcaggacgaa
     5101 ctgctggtag gggatctcga tatcgcgggt ggcagccggg gtcgcagcat gctgggcttc
     5161 gagcacgggc acgccggaaa cggccacgca ggcgcttgcc agcgcgagcc ggccgcagag
     5221 ccgtgggtac acgcaacctc ctgggacggg aacggtggga gccgactgac caggcctacg
     5281 acgcccgggc cgggatggtt tcactcctgg gacggcgtct a
//
LOCUS       DASRJQ010000038         4340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_255726, whole genome shotgun sequence.
ACCESSION   DASRJQ010000038 DASRJQ010000000
VERSION     DASRJQ010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255726"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(503..2344)
                     /locus_tag="VFB89_00890"
     CDS             complement(503..2344)
                     /locus_tag="VFB89_00890"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZM25892.1"
                     /translation="MSVQQQSTPATAQPSVSVQGLQICTDSGVPVVTDVSFDIAPGEV
                     LGLVGESGSGKTTVGLSLLGHARRGLRIAGGEILLDGENILALGEDELRRLRGSRVSY
                     VPQDPASSLNPALRIGLQLREVLVAHGWGNRQEQDERMAQMMREVALDEDPRYLRRYP
                     HQLSGGQQQRIGLAMAFANRPRLIVLDEPTTGLDVTTQATVLSTVRDLAALHGVAALY
                     VSHDLAVVAALARWVAVMYAGRIVELGAAEELFESAAHPYTRRLVGAIPRLTGGRSLV
                     GIPGHAPSPGRRPPGCAFAPRCKLHIPQCDENVPALDPVRPGHRVRCIRAHDVLKQAR
                     AAVGDPLDLAAASTDSAALTLENVVAHYGPVEVLHSINLTLERHECLAIVGESGSGKT
                     TTARSIAGLHRDWTGSIRLGDQELEESARARSTESRRQIQYIFQNPYGSLNPRKTIGE
                     TVGQPLSVFGIAKRREADKMVWEMLEQVSLPANYANRYPDQLSGGERQRVAIARALIS
                     SPSILICDEVTSALDVSVQAAIVELLGKLQRELGLSMVFVTHNLPLVRSIAQKVAVLS
                     EGNIVEYGETAEVLADPQQPYTRELIANTPSLETATSEAIAKEATSG"
     gene            complement(2341..3252)
                     /locus_tag="VFB89_00895"
     CDS             complement(2341..3252)
                     /locus_tag="VFB89_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM25893.1"
                     /translation="MSQTEQAPALAEGVDTPTPPGRPSSLLVRSLRLWRTRIGVLFVL
                     ILVVIAIIGPWIAPHGASEFVAPPFSKPGQNGAGVLGTDYIGQDVYSRFLYGGRSILI
                     VAIVSTAIGLVLGIAIGLIAAYARNALDDVLMRAMDVIMAFPQILLALVAVSLIGAGW
                     GIIIAAIALTTAPRVARVTRGAALPVVERDFVAATESMGLSRSRILTGELFPSILGPI
                     MVEATLRLTYSIGVIAALAFLGLAPDPNGANWGTMIKQNQLALATQPWGALAPILAIA
                     LLTMGTGLIGDGIARTAAGIDRERSEE"
     gene            complement(3249..4220)
                     /locus_tag="VFB89_00900"
     CDS             complement(3249..4220)
                     /locus_tag="VFB89_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM25894.1"
                     /translation="MRWLRGGGISGFIVRRVLLGLLTLFLVSVVVFVATQALGDPARA
                     ILGRDATPERLTALQNQLNLDQSLVQQYLNWLGGLLHGDLGDSLAAQLPVSTLLHDRL
                     INSAVLVFCAAVVSIPLSIVLGSYAALRREKPFDTISTNIMLLLAALPEFVVAVLLVI
                     LFATTVFTWLPAISAVPPGTHPWDDPKILILPTLTLIIAVAPYVARIMRASMIEVLES
                     DYVEMARLKGLPERTVLRRHALPNAIGPVFQVIAINLAYLAGGIVVVEYVFAYPGIGS
                     ALLDAVVNHDIPVVQALAMLIAGVYVVVNLLADVATILVTPRLRTQL"
BASE COUNT          761 a         1349 c         1503 g          727 t
ORIGIN      
        1 gacgagaccg ccagcatgca ggagacccag caggcgctcg acgacgccca ggcgctcatc
       61 gacgcggcca accagttgct gcccagcctc gagatctttt gactcgaggg cggcggctcc
      121 gtggaatgac ttgtggaatg aaacggtgtc cgtgagcgtg gttcgaggcg gtcaaagtgg
      181 gtcccgaaac gacccattac gccttaagtc gactggagtc gccggtgacg gaccccctac
      241 ggatcgaaat gttggggttc gaatccctcc gagcgcgaca cctctgacca gcggaaacgc
      301 tgagatcaat cttcagatca gaccaatgac gagcagagat ctgaaggttg gttgacaccc
      361 gccgtgacgg tttcgagccg cgtgcgcggc cgcgtagacg gttcgaactc gagcggggtg
      421 agattgtgcc gagggcgagc attgggtctt gccaacctcc tgcagccgcc tcgatcacga
      481 cactcgatct ctatagagcc ggctagccgc ttgtggcttc cttggcgatg gcctcggagg
      541 tcgcggtctc gagtgagggt gtgttggcga tcagttccct ggtgtacggc tgctgggggt
      601 cggcgagtac ttcggcggtt tcgccatact cgacgatgtt gccttcggac agcacggcca
      661 ccttctgcgc gatcgatcgc accagcggga ggttgtgagt gacgaagacc atcgatagac
      721 cgagctctcg ctgcagcttg cccagcagct ccacaattgc ggcttgcacg ctcacgtcga
      781 gcgccgacgt cacctcgtcg cagatcaaga ttgagggcga ggagatcaga gctcgtgcta
      841 ttgcgacgcg ctgccgctcc ccgcccgaaa gctggtcggg atagcggttg gcgtagttcg
      901 ccggcagtga gacctgctcc agcatctccc agaccatctt gtcggcctcg cgacgcttgg
      961 cgatgccgaa gactgacaag ggctgtccca ccgtctcgcc gatcgtcttg cgcgggttaa
     1021 gcgagccgta cgggttctgg aagatgtatt ggatctggcg gcgggactcc gtactgcgtg
     1081 cccttgccga ctcctcgagc tcctgatcgc cgagtcggat gctgccggtc cagtcgcggt
     1141 gcagcccggc tatcgatcgc gccgtggtgg tcttgcccga tccggactcg ccgacgatgg
     1201 cgaggcactc gtgacgttcg agggtgaggt tgatcgagtg cagcacctcg accgggccgt
     1261 agtgagcgac gacgttctcg agcgtgaggg ccgcggagtc tgtgctggcg gcagcgagat
     1321 cgagtggatc gccgacggcg gcccgggcct gcttgagaac gtcgtgcgcg cggatgcacc
     1381 gaacccggtg gccgggtcgt accgggtcca gcgctgggac gttctcatcg cattgtggga
     1441 tgtgcagctt gcagcgaggc gcaaaggcgc agcccggtgg ccgacgaccc ggcgaaggcg
     1501 catggccagg gattccgacc aggctgcggc cgccggtcaa gcgtgggatc gcgcccacca
     1561 gtcgccgggt gtaggggtgg gcggcggatt cgaacaactc ctccgccgcg ccgagctcga
     1621 cgattcgccc ggcgtacatc acggcgaccc agcgggccag ggcagcgacc acggcgaggt
     1681 cgtggctgac atagagcgcg gcgacgccgt gaagcgcggc gagatctctc acggtcgaca
     1741 gcactgttgc ctgcgtcgtg acgtcaagac cggtggtcgg ctcgtcgagc acgatcagcc
     1801 gtgggcggtt cgcgaatgcc attgccagac cgatgcgctg ttgctgtccg ccagacagct
     1861 ggtgcggata gcgacgcaga tatcgtggat cttcatcgag tgcgacctcg cgcatcatct
     1921 gcgccatccg ctcgtcctgc tcctgccggt tgccccagcc gtgggcgacc aggacctcgc
     1981 ggagctggag gccgatccgc agtgctgggt tgagcgagga ggcggggtcc tgcggcacgt
     2041 atgagacgcg cgagccgcga agtcggcgca gctcgtcctc gccgagagcg aggatgttct
     2101 ctccgtccag caatatctcg ccgcccgcga tccggagccc gcggcgggca tggcccagca
     2161 gcgacagtcc tacagtggtc ttgccggatc cggactcgcc gaccagtccg agcacttcgc
     2221 cgggcgcgat gtcgaaggag acatcggtca cgactgggac gccggagtcc gtacagatct
     2281 gcaggccttg cacgctcacc gagggttgcg cggtggccgg tgtcgactgc tgctggacgc
     2341 tcactcttcg ctccgctcgc ggtcgatgcc cgccgcggtg cgggcgatgc cgtcgccgat
     2401 cagtccggtg cccatggtga gcagagcgat tgccaggatg ggcgccagcg cgccccatgg
     2461 ctgtgttgcc agggcgagtt ggttctgttt gatcatggtt ccccagttgg cgccgttcgg
     2521 gtcgggtgcg agtccgagaa aggcgagcgc cgcgatgacg ccgatcgagt aggtgagccg
     2581 cagggttgcc tccaccatga tcggaccgag gatgctgggg aacagctctc cggtcaggat
     2641 ccgcgaccgg gacagaccca tcgactcggt ggcggctacg aaatcgcgct ccacgaccgg
     2701 cagggcagcg cctcgtgtga cccgggccac gcgaggcgcc gtggtcaggg cgatggcggc
     2761 gattatgatc ccccagccgg cgccgatcag actgaccgcg acgagcgcga gcaggatctg
     2821 gggaaaggcc atgatcacgt ccatcgcccg catgaggaca tcgtcgagcg cgttgcgcgc
     2881 gtatgcggcg atgaggccga tcgcgatgcc gaggacgagt ccgatcgcgg tggagacgat
     2941 ggcgacgatc aggatgctgc gtccgccgta taggaagcgg ctgtagacat cctggccgat
     3001 gtagtcggtg cccagcacgc cggctccgtt ttggcccggc ttcgagaacg gcggtgcgac
     3061 gaactcggac gcgccgtgtg gcgcgatcca ggggccgatg atcgcgatta cgaccagaat
     3121 gagcacgaac agcacgccga tcctggtgcg ccaaagccgg agtgagcgga ccagcagcga
     3181 ggacggacgt cctggcggtg tgggggtgtc gacgccctcg gccagagccg gcgcttgttc
     3241 ggtctggctc atagttgagt ccttagccgt ggcgtgacga ggatggtcgc gacgtccgcc
     3301 aacaggttca ccaccacata cacaccggcg atgagcatgg cgagcgcctg caccaccgga
     3361 atgtcatgat tgacgacggc gtccagcaac gcgctcccga tccctgggta ggcgaatacg
     3421 tactcgacga ccacgatgcc gccggcgagg taggccaggt tgatcgcaat gacctggaag
     3481 actgggccga tcgcgttggg cagcgcgtgc cggcgcagca ccgtccgctc cggcaggccc
     3541 ttgagccggg ccatctcgac gtagtcgctc tcgagcactt cgatcatcga tgcgcgcatg
     3601 atgcgggcta cgtacggcgc gaccgcgatg atcagggtca gggtcggcag gatcaggatc
     3661 ttcgggtcgt cccacggatg tgttccgggt gggacggcgc tgatcgccgg aagccaggtg
     3721 aacaccgtgg tggcgaagag gatcaccagc aggaccgcga cgacgaactc ggggagcgcc
     3781 gccagcagca gcatgatgtt ggtgctgatc gtgtcgaacg gcttctcgcg ccgcaaagcg
     3841 gcatacgaac ccagcactat cgacaagggg atcgatacca cggcagcgca gaacaccagg
     3901 accgcggagt tgatcagccg gtcgtgcagc agggtggaca cgggcagctg tgccgccagc
     3961 gaatccccca gatccccgtg caatagcccg ccgagccagt tcaggtattg ctggaccagc
     4021 gattggtcga ggttcagctg gttctgtaag gcagtcaacc gctccggtgt cgcgtcgcgg
     4081 cccaggatcg cccgcgccgg gtcaccgagc gcctgggtag cgacgaacac cacgaccgac
     4141 accaagaaca gcgtgaggag gccgagcagg acccgacgga cgatgaaccc actgatgccg
     4201 ccgcctctga gccaccgcat tgtcggccgc ctctcaactc aggacgagaa cccaattgtc
     4261 cggaacccgc gtccgaagct gtcgaggttg agggtgccct tgctgggctc gagacccgcc
     4321 acgttggcgg cataaccgtc
//
LOCUS       DASRJQ010000039         4149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1109405, whole genome shotgun sequence.
ACCESSION   DASRJQ010000039 DASRJQ010000000
VERSION     DASRJQ010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109405"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VFB89_00905"
     CDS             complement(<1..430)
                     /locus_tag="VFB89_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25895.1"
                     /translation="MPNLSADDRVLVPALAALGLRAEAVVWDQPDVPPGVTAVVIRSC
                     WDYHLRIDEFFSWLSRLERRGVRVLNPPPLIRWNADKHYLLDLSSRGVATVPTRVIRK
                     GERGRLAGVLAEAGFEEAVVKPAVSASAHATWRTSRALAHH"
     gene            complement(665..1549)
                     /locus_tag="VFB89_00910"
     CDS             complement(665..1549)
                     /locus_tag="VFB89_00910"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HZM25896.1"
                     /translation="MTGIRSVTAFLLASAVALIGCGDSQGPGTARFSVKLTDAPGPFK
                     TAKVTIAEVYLQGSGGKTSLSSTPTTVNLLELQNATLDLVKDVEVPTGSYNQLRIVLT
                     GGYVELQDGSIFASSADYEGLPPGAVATGDLQMPSLGQTGIKVTLPGTALEVTGPQKI
                     VLLDFDVSQSFGHEASGWVMHPVIKGAEIETTGSVSVSLKLKDGVTLPEVNGNQVTLA
                     DFTATLGTETQALDANGNTSFAFVLPDTYPLYFTGPAGLTFTTDPVATQANPIQVVVT
                     SGQPTSASATITSATAAP"
     gene            1714..2259
                     /locus_tag="VFB89_00915"
     CDS             1714..2259
                     /locus_tag="VFB89_00915"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZM25897.1"
                     /translation="MTDRSDPALIRQVLDGNPDAYGALVARYRERYSRFAVHMLGERE
                     DAEEALQDAFLRAYRSLAQCADPARFDAWLFRILANRCRTAGARRARRARTFVRDEVA
                     LGEASAEHPAERREWREEIHRALGRLEPDQREAFLLKHVEDLSYEEMSTLTGAGISAL
                     KMRVKRACERLRVMLGEVQHA"
     gene            2252..2842
                     /locus_tag="VFB89_00920"
     CDS             2252..2842
                     /locus_tag="VFB89_00920"
                     /inference="COORDINATES: protein motif:HMM:NF027875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HZM25898.1"
                     /translation="MHDDDQHDELITRIADELRTPVALDPGFDARVMAQVRRSARARW
                     RLALRWWTEPRPLRVSPLGGLACAAGVAALFVVGLVSLRPASPSPIHEAVTPGKVIAA
                     EPGTEMVQFVLVAPQASTVSVVGDFNDWDPSRTPLQAEAAGGVWSVNVPLRSGQHQYA
                     FVVNGKEWRPDPTAPRAVTDDFGSPNSVIVVGSKST"
     gene            2839..3504
                     /locus_tag="VFB89_00925"
     CDS             2839..3504
                     /locus_tag="VFB89_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25899.1"
                     /translation="MRYLLFFAILVAAAPGLAAQDPRLEARLDPTTRAVVAALVDSAR
                     AEGLPTEPIVDKALEGTTKRASSDRIVAALRTLSTDLRLARGALGRTAPDGDVIAGAS
                     AIRAGASPETLERINGVRGKAGISVPLAVFADLVARGVPADTASTVILHLAEGGASDA
                     DYAGLERSVTRDIGAGAPPGASAAIRARVTTGDVGAATQGGPPPGVPVSPKDPKGKPS
                     NRP"
     gene            3530..>4149
                     /locus_tag="VFB89_00930"
     CDS             3530..>4149
                     /locus_tag="VFB89_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25900.1"
                     /translation="MPGRVCLALIALALSYPTTRAPAQAVGSLDAGITRVEYNDIPAL
                     TAYSLAPTFQLFRANGSLLANSVFSRFTDGRWTLQGTSIGSIFVPRGSAVQAELGGTL
                     AGSVDDDGNKSGEALGQARLHFGGARRGLWVGGGLGRGYDGTAWQTLGLIEAAGWARI
                     GDVTAVLSTSPNWVGSDLTFFDTQATARLVHGPLELSGYGGIRRWF"
BASE COUNT          729 a         1313 c         1363 g          744 t
ORIGIN      
        1 cgtggtgtgc caaagctcgc gaggtgcgcc aggtggcatg ggctgaagca gaaacggccg
       61 gcttcaccac tgcttcttca aacccagctt ccgcgaggac acccgcgagc cgaccccgct
      121 cgcctttccg gatgactcga gtcggcaccg tggccacgcc tcgcgaagag agatccagca
      181 gatagtgctt gtcggcgttc cagcgaatga ggggcggtgg attcaacacg cgaacaccgc
      241 ggcgctcgag gcgggaaagc caggaaaaga attcgtcaat ccgaagatgg taatcccagc
      301 acgatcggat aacgaccgca gtcacgccgg ggggaacatc aggctggtcc cacaccaccg
      361 cttctgctct gagaccgagc gccgcgaggg ccggcacgag cacgcgatcg tcggcggaaa
      421 ggttcggcaa gcgccggtag gtagcgagcc caacccgcct cacgctgacg tcccgtcgaa
      481 cgccgcgatc gcgggcgcat ggtatctggt aagggtccgc atccgtaggt cgaatcgggc
      541 tggggaacga taagcgggcc ggcggtccgc tgcctccgtc acgcaggcac acttgcaaag
      601 cagcaggagc tccgaatttc ggagctcctg cgcgttctcc agcgtcaatc ctcgcttgtg
      661 gagattacgg cgccgcggtt gcgctggtga tagtagcgga tgcgctcgtc ggctggccgg
      721 acgtcaccac tacctgaatg gggttcgcct gggtagcgac cggatcggtg gtaaacgtga
      781 gccccgccgg gccggtaaag taaagcggat acgtatcggg tagaacaaag gcgaaactgg
      841 tgtttccatt ggcatccagg gcctgcgtct cggtgcccag cgtagcggtg aagtcagcca
      901 gagttacctg gttcccattc acctcaggca gggtgactcc atccttcagc ttgagcgaca
      961 ccgacacgct gcccgtagtc tcgatctccg cgcccttgat gacggggtgc atgacccatc
     1021 cggaagcctc gtggccgaag ctctgggaca cgtcgaagtc gagcagcacg attttttgag
     1081 gcccggtaac ctccaaggca gtccccggca gggtgacctt gatcccggtc tgccccaggc
     1141 tcggcatctg gaggtcgccg gtcgcaaccg cgccgggcgg caacccttca tagtccgccg
     1201 aggacgcgaa gatgctgccg tcctgaagct ccacgtagcc tccggtcagg acgatccgga
     1261 gctgattgta actcccggtc ggtacttcga cgtctttgac caggtcgagc gtcgcgttct
     1321 gaagctcgag caggttcacc gtcgtcggag tgctgctgag gctggtcttt ccgccactgc
     1381 cttggagata cacctcggcg atcgtcactt tggcggtctt gaagggtccg ggggcatcag
     1441 tcagcttcac cgagaaacga gcggtgcctg gcccctgtga gtcgccgcag ccgatcagcg
     1501 caaccgcaga agctaagagg aacgccgtta cagaacgaat accagtcatg atcccaagct
     1561 cctgtaaaat ggctgtaggt gttccaagta cagactccgt ggccgttccg gacagtcaca
     1621 gttgccggtg gccgtgggca gtgcagtatc ttgcgtgatg ttaaaggctt agagcctcgt
     1681 gacccctggc ccattcaagg agtctgtacc ccggtgacgg atcggtccga ccctgctctc
     1741 atccgccaag tgctggacgg caacccggac gcatacggag cactggtggc tcggtaccgc
     1801 gagagatact cgcgatttgc cgtgcacatg ctgggggaaa gggaggacgc cgaggaggcg
     1861 ttgcaggatg cgttccttcg ggcctaccgc tcactggcgc aatgtgctga tccggcccgg
     1921 ttcgacgcct ggttgttccg catcctcgca aaccgttgcc ggaccgcagg tgcccgcaga
     1981 gctcgtcgag cgcgaacgtt tgtacgggac gaggtcgcct tgggggaagc ttcagccgag
     2041 catccggctg agcggcggga atggcgggaa gagatccacc gggccttggg gcggctcgag
     2101 ccggatcaac gggaggcctt cctgctgaaa cacgtggaag acctgagcta cgaagagatg
     2161 tccactctta ccggagcggg catttcggcc ctgaagatgc gtgtgaaacg cgcttgtgaa
     2221 cggctgcggg tgatgttggg agaggttcaa catgcatgac gacgaccagc acgacgagct
     2281 gattacccgc attgcggacg agctgcgcac gccagtggcg ctcgatccgg gcttcgacgc
     2341 ccgggttatg gctcaggtgc gtcgctcggc gcgggcgcgc tggcgactcg cgctgcgctg
     2401 gtggacggag ccgcggccgc ttcgggtgtc gccattgggt gggctcgcat gtgcagcggg
     2461 agtcgccgcg ctgttcgtcg tcggactcgt gtcgctccga cccgcctcgc cgtccccgat
     2521 acacgaagcc gttactccag gcaaggtcat tgccgccgaa ccaggcaccg agatggtgca
     2581 atttgttctg gtcgcgcccc aagcctctac cgtttcggtg gtcggcgatt tcaacgactg
     2641 ggatccgagc cgcacgccgt tgcaggccga agccgccggc ggggtctggt cggtcaacgt
     2701 gccgcttcgc tcgggtcagc atcaatacgc cttcgtggtc aacggcaagg aatggcgacc
     2761 ggatccgacc gccccccggg ccgttacgga tgacttcggg tcgcccaatt ctgtgatcgt
     2821 ggtaggcagc aagagcacgt gagataccta ctcttcttcg ccatcctagt tgccgcggca
     2881 cccggtcttg ccgctcagga tccccgtctg gaagccaggc tcgatccgac aactcgcgca
     2941 gtcgttgcgg cactggtgga ctctgcccgc gccgagggcc tgcctaccga gcctatcgtc
     3001 gacaaggccc tggaaggaac gaccaagcgg gcgagcagcg accgcatcgt ggcagcgctt
     3061 cgcacgcttt ctaccgacct ccgcctagct cgtggtgcac tcggtcgcac ggctcccgac
     3121 ggtgacgtga tcgccggggc gagcgcgatt cgggcaggcg ccagcccgga gactctcgag
     3181 cgcatcaacg gtgtccgagg gaaagccggt atctcggtgc cgctcgcggt ctttgccgac
     3241 ctggttgcgc gtggcgttcc ggccgatacc gcatcgacgg tgattctcca cctggctgaa
     3301 ggcggcgcaa gcgacgcaga ctacgccggc ctggagcgca gcgtgacgcg tgatattgga
     3361 gccggcgcgc cgcccggtgc ctctgcggca attcgtgccc gagtcaccac cggtgatgtg
     3421 ggtgccgcca cgcagggcgg cccgcccccg ggcgtgccgg tcagccccaa agacccaaaa
     3481 ggaaagccca gcaaccgtcc ctaattccgg cacgcgttat ccttagctcg tgccgggacg
     3541 cgtctgcctc gcgcttatcg ccctcgccct ctcgtatccg accacccgag cgcctgcgca
     3601 ggcggtcggc tcgttggatg caggtatcac tcgcgtagag tacaacgaca tccccgccct
     3661 cacggcctac agtctcgctc ctacattcca gctctttcgc gccaacggat cgcttctcgc
     3721 caatagcgtg ttctcccgct tcactgacgg acgatggacc cttcaaggca cctcgatagg
     3781 ctcgatcttc gtgcctcgag gctcggcggt acaggcggag ctgggcggaa cgctcgccgg
     3841 cagcgtggac gatgacggca acaagagcgg cgaggcgctg ggccaggccc gtcttcactt
     3901 cgggggcgct cgccgcggct tgtgggtcgg aggggggctg ggcagagggt acgacggaac
     3961 cgcctggcag acacttggat tgatcgaagc cgcgggctgg gcccgaatag gggacgtcac
     4021 cgcggttctc agtacctcgc cgaattgggt ggggagcgat ctcacgtttt tcgataccca
     4081 ggcaacggcg cgtctggtcc atggaccact cgagctgagc gggtacggcg gcattcgccg
     4141 gtggttcga
//
LOCUS       DASRJQ010000040         2486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3632073, whole genome shotgun sequence.
ACCESSION   DASRJQ010000040 DASRJQ010000000
VERSION     DASRJQ010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3632073"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..>2486)
                     /locus_tag="VFB89_00935"
     CDS             complement(168..>2486)
                     /locus_tag="VFB89_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25901.1"
                     /translation="GDAKHFGNGILQAHSALGVAPDLNRPRSRTSDVSFALFRLITGL
                     GLAETPVREQMFNLELEQRVLLNPRLQEIVPDPDRDGALKPSELRELMTAIIEDSEAS
                     DALRKHVAMRYPAVTGRSAPASPKTKGIVAEVVRACDAVPQLRDPAYRRLRVYAVDPS
                     FSTRLETAGIDETTLQVRWESLEPASRSRRARPPTAAGGKEPGNREPGPMGEYFEIDD
                     VDPADAFYGMVNLDDSRLLAQDGLPPSEGNPHFHQQMVYAVAMSTVQQFERALGRPVF
                     WRPMSDRTFRRRLTLRPHALRQANAFYSPENVALLFGYFDADADDPGTDLPGGRIYSC
                     LSHDIIAHETTHAILDGMQRNYNQPTNPDVLAFHEAFADLVALLQHFALTDVLEHEIM
                     RTRGNLETESVIGTLAIQFGRASGNRAALRDAIGHIEDGKWQRLQPDPAQYETTMTPH
                     ARGALLVAAIFDAFLAIYNTRTADLVRLSSGGSGVLPAGAIHPDLVHRLCEEASKSAK
                     HVLTMIIRALDYLPPVDVTFFEFLRAVITADLDLVPDDRLNYRVALIDAFRRRGIYPK
                     DTSSGTSAAGRTLSVETLRWRSPDPTRRPEQWDAIEASYMEIAKSLKPYADQHLYLKE
                     RERRFSRKIIRQLKLKEQFQDAFKEVPDFAREVGLSPDHDFEVEELRSAMRIGPDGQN
                     IPQVLISLTQTVQVRSNGQSYRFPGGSTLIIDLTVPTVRYCVSKNVDNTATRKRTAEF
                     FAQTQRDPLHALFFRPDPNRPFAALHQLSRAL"
BASE COUNT          446 a          758 c          807 g          475 t
ORIGIN      
        1 gcgacgggcg cccggctaac aaagcgacct gatcgcaaca ggcaccagac cgaggtggca
       61 ttttcacgag acggatgacg cttccacaag tccgggtgct tgatcaagat caccgaataa
      121 cgtgtgggcg cgtcagcttg caagtgtcgt ggaacgaccc tagttcgcta tagcgctcgc
      181 gagagctgat ggagtgcggc gaatggccgg ttcgggtctg gccggaagaa gagtgcatgc
      241 agcgggtctc gctgcgtctg agcgaagaat tcggcagtgc gtttccttgt cgccgtatta
      301 tcgacgttct tgctcacgca gtaacgaacg gtaggaacag tcaggtcgat gatcaaggtg
      361 gagccaccgg ggaatctata gctctggccg ttgctacgca cctgaacagt ctgagtcagt
      421 gatatcaaca cctgcgggat gttttgacca tcagggccga tccgcattgc actgcgcaac
      481 tcctccactt caaagtcgtg atctggactg aggccgacct cgcgcgcgaa atcgggcact
      541 tccttgaagg cgtcttggaa ttgctctttg agctttagct gccgaatgat ctttctgctg
      601 aatcgccttt cccgttcctt gagatagagg tgctggtcgg cgtacggctt gagactcttg
      661 gcgatctcca tgtagctcgc ctcgatggcg tcccattgct cgggccgtcg agtcgggtcg
      721 ggtgaacgcc agcgcagagt ctcgactgaa agcgtgcgcc cagccgccga cgtcccgctg
      781 gaggtgtcct tcggataaat cccgcgccgg cggaacgcgt cgatgagcgc gacccggtag
      841 ttgaggcggt cgtctgggac caggtcgagg tcggcggtga tcacagcgcg aaggaattcg
      901 aagaaggtca cgtcgaccgg cggcagatag tccagtgccc ggatgatcat ggtgaggaca
      961 tgttttgcgc tcttgctggc ttcctcgcag agacggtgca ccaggtcggg atggatcgct
     1021 ccggccggca agacgccgct gccaccgctg gacagccgca ccaggtccgc ggtgcgggtg
     1081 ttgtagatcg cgagaaacgc gtcaaagatc gcagcgacca gcaacgcccc gcgtgcgtgc
     1141 ggcgtcattg tcgtctcgta ctgcgctgga tccggctgca accgctgcca cttcccatcc
     1201 tcgatgtggc cgatagcgtc ccggagcgcc gctcggttgc cgctagcgcg gccgaactgg
     1261 atcgccagcg tgccgatcac cgactccgtc tccaggttcc cgcgggtccg catgatctcg
     1321 tgctcaagga cgtcggtgag ggcgaagtgc tgcagcagcg cgaccaggtc ggcgaaggcc
     1381 tcgtgaaagg caagcacgtc ggggttggtc ggctggttgt aattgcgctg cattccatcg
     1441 aggatcgcgt gggtcgtctc atgagcgatg atgtcgtgcg acaggcacga gtagatgcgg
     1501 ccgcccggca gatccgtgcc cggatcatca gcgtcggcgt cgaagtaacc gaagagcagc
     1561 gcaacattct ccgggctata gaaagcgttg gcctggcgca gcgcatgcgg gcgcaacgtc
     1621 agccgccgcc ggaaagtgcg atcagacatc ggccgccaga agaccggtcg gccaagcgcc
     1681 cgctcgaact gctgcactgt gctcatcgcc accgcgtaca ccatttgctg gtggaagtgc
     1741 gggttgccct ccgaaggcgg caagccatcc tgtgccagca gccgtgagtc atccaagttg
     1801 accatcccgt agaacgcgtc ggccggatcc acatcatcga tctcgaagta ctcgcccatc
     1861 gggccgggct ctcggttccc gggctccttc ccgcctgccg ccgtcggcgg ccttgcccta
     1921 cgcgatctgc ttgcaggctc caagctctcc cagcgaacct gcaaggtcgt ctcgtcgatg
     1981 ccagccgtct ccaggcgagt ggagaagctt ggatcgaccg cgtacacgcg caaccggcgg
     2041 taggccgggt cgcgcagctg cggtaccgcg tcgcaggcac ggaccacctc agcgacgatc
     2101 cccttcgtct tcggtgacgc tggggccgag cggccagtga ccgccggata ccgcatcgcg
     2161 acgtgcttgc ggagtgcgtc tgaggcctct gagtcctcga tgatcgccgt cataagctcc
     2221 cgcagctccg aaggtttcag cgctccgtcg cgatccggat ccgggacgat ctcttgcagc
     2281 cgcgggttga gcaggacccg ttgttcgagt tcgagattga acatctgctc gcgcaccggc
     2341 gtctccgcca gaccgagccc agtgatgagg cggaaaagtg cgaagctcac gtcggaggtt
     2401 cggctgcgag gccggttgag gtccggcgcg acgccaagtg ctgaatgtgc ttggagaatg
     2461 ccgttgccga agtgcttggc atcgcc
//
LOCUS       DASRJQ010000041        10090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_813295, whole genome shotgun sequence.
ACCESSION   DASRJQ010000041 DASRJQ010000000
VERSION     DASRJQ010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 10090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10090
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813295"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..408
                     /locus_tag="VFB89_00940"
     CDS             184..408
                     /locus_tag="VFB89_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HZM25902.1"
                     /translation="MAWDYIEGKWKEMRGKVRERWGKLTDDDLDVIAGKRDQLVGLLQ
                     QRYGLARDEIDRQIEDFERSNMATSGRSAR"
     gene            448..603
                     /locus_tag="VFB89_00945"
     CDS             448..603
                     /locus_tag="VFB89_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HZM25903.1"
                     /translation="MLWTIFVILLVLWALGMVSSYTLGGFIHILLVIALVILLINLLS
                     GRSRPVV"
     gene            847..1356
                     /locus_tag="VFB89_00950"
     CDS             847..1356
                     /locus_tag="VFB89_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25904.1"
                     /translation="MSSSITPIRIAPISDRMIRRKRRRPRLGLLACEVLSLAALFTIL
                     VPLTAQVRLPSVGRFLVSWPDLSDAHAICRQFVKRAEAGQEESTSREVGNWGWRRLDD
                     GRFRILGALDHPTVSGGIRRIRYQCDLAPLNSQGRWRVDSLAFSRESIGASTALAPAV
                     AVKAGSADR"
     gene            complement(1331..1981)
                     /locus_tag="VFB89_00955"
     CDS             complement(1331..1981)
                     /locus_tag="VFB89_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZM25905.1"
                     /translation="MLRGLLQDSLVADDEDVVGRWQRIQRPGVGRWLLIATLVLVLPI
                     LVIAGILFGAGRSLALGAVQHLAARKFPALEWVEAEELEHWRSDSSRPRPVVLDARTS
                     PEYVVSHLKGALRIDPRTPSLRTVAAFPRDTPVVVYCTVGYRSARVGQWLRRQGFHSV
                     YDLAGGLFGWANQGRPMEADGRPVLQVHPYNALWGRVLKPEARADRPPVTDPLSLP"
     gene            complement(2047..2694)
                     /locus_tag="VFB89_00960"
     CDS             complement(2047..2694)
                     /locus_tag="VFB89_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25906.1"
                     /translation="MGLVSRLLATAALAALIAGAHCGNRPGSDCPLQIRQYYLSHKAP
                     QVVVRNTATYPVGRIVVHAAYQDLFNTYHEPTQVFDTILQPEQQVTLALPPIQGSIEW
                     ESLSLFATCQAVGQLDPAAGMAHLDVMPTYVARVDRDGEVLLERADVTVEILSDGDWR
                     ARFFIPPGSKLARGAKLNFAFADGRAGQAHVDHVHRALAKKGPRLVEVAGVGPLG"
     gene            complement(2902..3438)
                     /locus_tag="VFB89_00965"
     CDS             complement(2902..3438)
                     /locus_tag="VFB89_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25907.1"
                     /translation="MLPRSIAAFLAVALVSCGATGTEPTLSDAKAGNPVAKHTVSFIQ
                     AQAFANFIPTGTVNWELFVARYVTKGERPDSTILIVDHCPQDCEVTDPSEVTVVFRGS
                     IPDKDFKVGRWQRDWELHTAIPGQGRIDVTFHNEGNWSPPSGTQSIPSGLSGTVVGVN
                     ANGGFVTLFFDPRGLPVP"
     gene            complement(3762..4193)
                     /locus_tag="VFB89_00970"
     CDS             complement(3762..4193)
                     /locus_tag="VFB89_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZM25908.1"
                     /translation="MWFERAAVQQPAVDAPVVLLVERHNSLRHLLDELLTEAGYRVLE
                     AGSRPALLGQLREATRVDVIVTDTGLRLPGWEVVQEATGAWPGVPVVRLVGSAADAVP
                     VYGEDPSTMTLVTKPFVITELLEVLDRLVPTRRPRRARWSR"
     gene            4577..5602
                     /locus_tag="VFB89_00975"
     CDS             4577..5602
                     /locus_tag="VFB89_00975"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZM25909.1"
                     /translation="MTATAELQQLSPALFFDTMNAYQRSAALKAAIELNLFTAIGEGH
                     HTASDLAGQCQASERGTRILCDYLVVIGFLTKERDRYELTPDSRAFLDRHSPAYLGTA
                     VEFLNSAFVTDGFKDMAAVVRRGGTVLVEGGGHLGPDDPMWVNFARAMAPLMQLPAER
                     LAQLLDADAAPAWKVLDIAAGHGLYGIALARHNPRAEVVALDWQNVLGVAQENAQAAG
                     VAQRYRPLPGSAFDVEFGSGYDLVLLTNFLHHFDPQTIEHLLRKVRAALKPGGRAVAL
                     EFVPEEDRVSPPTAAAFSLIMLAGTPGGDAYPYSEYERMFRNAGFTGTELHDLPPTFF
                     RVVVAPN"
     gene            5967..6479
                     /locus_tag="VFB89_00980"
     CDS             5967..6479
                     /locus_tag="VFB89_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25910.1"
                     /translation="MQSFAKYLVEIGVLPSNALRDVKAPRANPPRCRFLELPDVLRVV
                     EGSEEPYRAIFAVAHGAGLEVSAILALVESDVDHQRREIRARGEKFWTRDRIARVADW
                     AWPIVEAHWRSVLPGERLFRGVDRWEAGDFHRERLRALGVPHHTLHDSRHHWAVRMVR
                     AGMPLELVAR"
     gene            complement(6669..6742)
                     /locus_tag="VFB89_00985"
     tRNA            complement(6669..6742)
                     /locus_tag="VFB89_00985"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6706..6708),aa:Met,seq:cat)
     gene            complement(6788..7081)
                     /locus_tag="VFB89_00990"
     CDS             complement(6788..7081)
                     /locus_tag="VFB89_00990"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HZM25911.1"
                     /translation="MTPGRWAAIAVLLAAVVFALQGGEYSTRDLFKLESQVASEAAEV
                     ARLQEVVDSLTRAAVAIERDPRVQERVARESFGMIRKGEFLYKIVRPDSAAGH"
     gene            complement(7078..8358)
                     /gene="eno"
                     /locus_tag="VFB89_00995"
     CDS             complement(7078..8358)
                     /gene="eno"
                     /locus_tag="VFB89_00995"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HZM25912.1"
                     /translation="MSTIIEVHAREIIDSRGNPTVEADVVLSSGAQGRAAVPSGASTG
                     EHEAVELRDGDTKRYGGKGVSEAVRNVNEVIGPRLEGMSASDQIAVDAEMMDLDGTPN
                     KSKLGANAILAVSLAVARAAASDTGLPLYRYLGGPMARVLPVPMMNILNGGAHASNNV
                     DVQEFMIVPIGAEAFSEALRMGVETFHALKKVLSKQGLSTAVGDEGGFAPMLPSNEAA
                     LDAVMQAIEAAGYQPGRDLAIALDPAASEFHQDGEYVFKKGDGSRRSAAEMVDLYTQW
                     VDRYPIVSIEDGLAEDDWEGWTLLTERLHDRVQLVGDDLFVTNVDRLGRGIEEGVANA
                     ILIKVNQIGTLTETLQCIELAKGSAYGVVISHRSGETEDTFIADLAVASGAGQIKTGS
                     ASRTDRIAKYNQLLRIGEELGELAHYPGRDLYPL"
     gene            complement(8390..9121)
                     /locus_tag="VFB89_01000"
     CDS             complement(8390..9121)
                     /locus_tag="VFB89_01000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZM25913.1"
                     /translation="MHALWSLACIVGFTLWHALRVSVAALVGVPDRAGGVYDREPRDW
                     ARRLISATGLPVSVDGLERLEAGRPYVFAANHASFVDIWILLASLPGSLRFIAKRELY
                     AIPLFGRGLRATGQIPVDRRDLDAATHSYENAAEIIRGGRSVIIFVEGTRSRDGRLGE
                     FKKGAFVLAIAAGVPLVPVYLGGTHWVLPPGSLCLRVRPVALIVGDPIPTEGLDYDDR
                     DDLLARARTWFQRQQADVDAARSTP"
     gene            complement(9122..>10090)
                     /gene="thiL"
                     /locus_tag="VFB89_01005"
     CDS             complement(9122..>10090)
                     /gene="thiL"
                     /locus_tag="VFB89_01005"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HZM25914.1"
                     /translation="IAMLPLGPGPEFDRVRAIARALGPLAAGLGDDCAILPEVSGSNL
                     VLSTDVSVEEVHFRREWLGPAEIGWRAAATALSDLAAAGAEAIGLLAAVTVPRDTREK
                     QLAELMQGVGEAVGSVGGVVLGGDLSGGPAWSLAITVVGRATRPLSRRGAKAGDGVWV
                     TGMLGGARAAVEAWRQGKEPEPEARRAFGRPEPRIAAGRALAARGATALIDLSDGLAS
                     DARHLAAASAVELRIELDRLPVAASAGKSAQAAGVSPAEFAARGGEDYELLASLPASF
                     DDAGAQAFLKETGLLLTRIGQVLTGTGTQLLLKGAEVTLTGFDHFG"
BASE COUNT         1835 a         3305 c         3211 g         1739 t
ORIGIN      
        1 ttgccgcgac ggtctcggcc caatgtggat cacgagtgct cggtgatgca gcgcgcgtcc
       61 gcattatgcc ctagggatta tcacaaaatc gacctttgag ggtctcttgc cgagcccgtg
      121 cgtaatcact accctcacac cgcagttcgc ggtcgacttt acgcctttct ttttggaggc
      181 agcatggcgt gggactacat cgaagggaag tggaaggaga tgcggggcaa ggttcgagag
      241 cggtggggca aactcaccga tgatgacttg gacgtgatcg cgggcaagcg ggatcagctc
      301 gtgggcctgc tgcagcagcg ttatgggctg gcgcgggacg agatcgatcg ccagatcgag
      361 gacttcgagc gcagcaacat ggcgaccagt ggtcgctcgg cccggtaggg cagacggccg
      421 ttcacccact ccttcaggag cgcatccatg ctctggacca tcttcgtcat cctgctcgtt
      481 ctgtgggcgc tcggtatggt gagctcctac acgctcggcg ggttcatcca cattctgctg
      541 gtgatcgcgc tggtcattct cctgatcaac ctgctgtcgg ggcggtctcg accagttgtg
      601 tagctggggc aggtgtcgga ttggacaaag caggacagca gcactcacga gcgccggttt
      661 tataccggcg ttcgtgcgtc gggcgcgtga cgtaagagac tccgagaacc gcagcccggt
      721 caccggcttc accggcgaat cgccacgtga gtgtaatcac ggcggcgaac ctcccggaga
      781 ggcagtctgt taggagcgtg acgctcgtag tcagagcggc gaggttgata caagtcgagg
      841 tgtgagatgt cgagctcgat aacgccgatt cggatcgcac caatctccga ccgcatgatt
      901 cgccggaagc ggcggcggcc tcgactgggt cttctcgcct gcgaggtgct gtcgctcgcc
      961 gccctgttca ccatcctggt gccgctgacc gcccaggtgc gcctgccaag tgtgggtcgg
     1021 tttctggtga gttggccgga cttgagcgat gcacacgcga tttgccgaca gttcgtgaag
     1081 cgagccgaag ccggccagga agaaagcacg agccgggagg tgggcaactg gggatggcgc
     1141 cggcttgacg atggccggtt ccgcattctg ggggctctcg atcacccgac cgtttccggc
     1201 gggattcgcc ggatacgata tcagtgcgat ctggcgccgc tcaactcgca ggggcggtgg
     1261 cgggtggaca gccttgcctt ctcgcgcgag agcattggcg cctcgactgc actcgcaccg
     1321 gccgtggcgg tcaaggcagg ctcagcggat cggtgaccgg cgggcgatct gcccgtgctt
     1381 caggtttgag tactcgaccc catagcgcgt tgtagggatg cacctgcagg accgggcggc
     1441 cgtccgcttc catcgggcgg ccctggttgg cccagccgaa gagcccgccc gcaagatcat
     1501 acacggagtg aaaaccctgc cgtcgaagcc actggcctac ccgagcgctt cgatatccca
     1561 ctgtgcagta gacgaccacc ggcgtatctc gcgggaaggc ggcgaccgtt cgcaagctcg
     1621 gggtgcgggg atcgatacga agagcgccct tgagatggct taccacgtat tcaggcgatg
     1681 ttctcgcatc caggacgact ggccgcggtc gcgatgagtc gctgcgccag tgttccagct
     1741 cttcagcctc cacccattcg agcgcaggaa atttccgagc cgccaggtgc tgaacggcgc
     1801 ccagcgcgag cgagcggccg gcgccgaaga gaatgcccgc gatcaccagg atcggaagca
     1861 ccaggaccag ggtcgcgatc aacagccatc ggccaactcc gggccgctgg atccgctgcc
     1921 agcgaccaac gacgtcttcg tcgtccgcca cgagcgagtc ctgaaggagt cctctcaaca
     1981 tcactggaca acggccaggg acgcaacgca ccggccgccg gttgcggagt tcaaggaatg
     2041 gctcgatcag ccgagcggtc cgacgccggc gacctccacc agccggggcc ccttctttgc
     2101 cagggcccga tggacgtgat cgacgtgggc ctgtcccgca cgcccgtcgg caaaggcgaa
     2161 gttgagcttg gcgcctcgcg cgagcttgct gccgggcggg atgaaaaagc gggctcgcca
     2221 atcaccatcg ctcaggattt ctactgtgac gtccgctcgc tcgagcagga cttcgccgtc
     2281 gcgatcgacg cgggcaacgt aggtgggcat aacgtcaagg tgggccattc ccgccgccgg
     2341 atcaagctga cctaccgcct gacaagtcgc aaacaaactg agtgactccc attcgatgct
     2401 gccctggatg ggggggagtg ccagcgtcac ttgctgctcc ggctggagga tcgtatcgaa
     2461 cacctgagtg ggctcatggt acgtgttgaa gaggtcctga taggctgcat gaacgacgat
     2521 ccggccaacg ggataggtcg ccgtgttgcg gacgacgacc tgaggtgcct tgtgggagag
     2581 gtagtactgt cggatctgga gcggacaatc agaaccaggg cggttgccac agtgcgctcc
     2641 ggcgataaga gccgcgagcg cggcagtcgc caacaatcgg ctaacgagtc ccaatgtggc
     2701 cttcttcgtc agtagccgga tggatcaagc cgatccaggt gttctagtga gttacttgtc
     2761 tagacgctct ggtgtggcgg gaaatacgtt cgcgccagcc accagtcagc ggggatagct
     2821 tgcttggaac cgggaaagaa gtggtacgac gtatatcgag gcctgcggcg atggcgacaa
     2881 caccgaatac cgctgaatga cttatggaac ggggagcccc cgggggtcga agaacagcgt
     2941 aacaaacccg ccgttggcat tcacgccaac caccgttccg ctgaggcccg aaggaatgct
     3001 ctgggtcccg gagggcggag accagttgcc ctcgttgtgg aaggtcacat cgatcctgcc
     3061 ctgccccggg atcgcggtgt gcagctccca atccctttgc catctgccga ccttgaagtc
     3121 cttatccgga atcgagccac ggaagacgac ggtaacctcg ctcgggtccg tcacctcgca
     3181 gtcttggggg cagtgatcga cgataaggat cgttgagtcc ggtcgctctc ccttggtcac
     3241 ataccgcgcc acgaacagtt cccagttgac cgtacctgtc ggaatgaagt tggcaaacgc
     3301 ttgcgcctgt atgaaactga cggtgtgctt agcgaccggg ttaccggctt tcgcgtcgga
     3361 aagcgtcggc tcggtgccgg tggcaccgca actgaccaag gcaacggcga ggaaggccgc
     3421 tatggagcgg gggagcatcg gtgatgtcct cgattccagg aaacaccgct agtagtcggt
     3481 agtaccgttg atcgcgagcg ctaagccgca tgctgggagc tgcaagaagc agcagcgcgt
     3541 cttatgccag cgaggtggga acgaaggact tgaagcgctt tcgcttggcg gcatcggggc
     3601 ttagatgccg ccaaccggtc gctcacacta tatcggcgta gggtttcgag gccacaggtg
     3661 gagaaagtct ttgtggtgac gtcgcaacgg gaccgaataa gaattttgca cccgtggcgc
     3721 cgtttgggct cagcggaccc ttcaacgtgc tgtgcgcttt tctatcgact ccacctggca
     3781 cgacgtggcc gccgggtggg gacgagccga tcaagtacct cgagcagctc ggtgatcaca
     3841 aacggcttgg tcacgagagt catggtcgag ggatcttcgc catagacagg cactgcgtcc
     3901 gctgcggatc caaccaaccg aacgacggga actccaggcc aggccccagt cgcctcttgg
     3961 accacctccc agcccggaag ccgaagtcca gtgtcagtca cgatgacatc gactcgcgtg
     4021 gcttcgcgca gttggccgag aagggcgggc cgagagcctg cttcaagcac gcgatagccc
     4081 gcttccgtga gcaactcgtc cagcaggtgg cgcaacgagt tgtgcctttc gaccagaagc
     4141 acgacgggtg cgtcgacggc gggctgctgt actgctgctc gctcgaacca catcggagga
     4201 tctcaggggc ctggggtaac gttgagatac ggcgcgattc gacaaccgtc aagccgtgga
     4261 aaccctaggc gatggcagta ggcctgtaag gagcgagcga gtgacctcaa ccccttgatt
     4321 ggcgactgca gcgcaaagaa cactgggcct ctccgccgct gcatcgcctc ccgagcacct
     4381 gctcgacgtg gacaccgaac agaaccgcca aggcacccag gacgagcagc aggcgccagc
     4441 cgacactcca gtagtacgca tcactaagtc gcccggcgat cttgacgctc ggcgcccctg
     4501 gcgtatcgtc gtactctctc tggaagagcc atattcccga cccgtcgccc acgcacactc
     4561 cctggggagc cgtcatatga ccgccactgc cgagctccag caactatcgc ccgcgctgtt
     4621 cttcgacacc atgaacgcct atcagcgcag cgccgcgctg aaggccgcca tcgagctgaa
     4681 cctcttcacc gcgatcggcg agggccacca caccgcgagc gatctggccg ggcagtgcca
     4741 ggcctccgag cggggcacgc ggattctgtg cgattacctc gtagtcatcg gattcctgac
     4801 caaggaacgg gaccgctacg agctcacgcc cgattcccgg gcgttcctcg accggcactc
     4861 gccggcgtat ctcgggaccg ccgtcgagtt tctcaactcg gcgttcgtga ccgatggatt
     4921 caaggatatg gctgccgtcg tgcggagagg cggtaccgta ctcgtcgagg gtggcggcca
     4981 tctcggtccc gatgacccca tgtgggtcaa tttcgcccgg gccatggccc cgctaatgca
     5041 gttgccggct gagcggctgg cccaactact cgacgccgac gcagctccgg cctggaaggt
     5101 cctggacatc gccgcgggcc atggactcta cggcattgcc ttggcacgtc ataacccgcg
     5161 cgccgaagtg gtcgcgctcg actggcagaa cgtgctgggc gtggcccagg aaaatgccca
     5221 ggctgcaggc gtcgcccagc gctaccgccc gctgccgggc agcgcgttcg acgtcgagtt
     5281 cgggtcgggc tacgacctgg ttctgctcac gaacttcctc catcacttcg acccgcagac
     5341 catcgagcac ctactccgca aggtccgggc agcgctcaag ccgggcggcc gagcggtcgc
     5401 cctggagttc gtccccgagg aggatcgggt ctcgccgccg accgccgccg cgttcagcct
     5461 gatcatgctc gccggcaccc ctggcggtga tgcctacccg tactccgagt acgagcggat
     5521 gttccggaac gcgggtttca cgggcaccga gctgcatgac ctgccgccca ccttcttccg
     5581 ggtggtcgtc gccccgaact gagctccggc ccacacaggc gccgtcccgc gtgctcatag
     5641 tacgtcgccg tcccaacacg cactggttcg tgcaagcgtt ccacggggac cagcgacaaa
     5701 ggcaccgcga aggccatgga gcggatgctc gaggacttgg gccacgtggc aagcgggcct
     5761 gggattgctc atgtgcggac gctgattccg gcgggcaagc cattcccccg ctctgccctc
     5821 actgggcccg ccgtggcgca atggctcgcc accaggacgg cgctcgcgca aaagcgccag
     5881 cgcagcgcga aacactcgcg acggtcgcga gaatcctccc cctcgcccgg tgagcggctc
     5941 gacccaaacg gcgctacctg gccgccgtgc agtccttcgc caagtacctg gtggaaatcg
     6001 gcgtgctgcc atccaatgcg ctgcgcgacg tgaaggcgcc gcgggcgaat ccgccccgct
     6061 gcaggtttct cgagctgccc gacgtcctcc gagtcgtgga gggatcggag gagccctacc
     6121 gcgccatttt cgccgtggcc cacggggctg ggctcgaggt gtccgcgatc ctggcgctgg
     6181 tggaatctga cgtggaccac cagcggcggg agattcgggc tcgaggagaa aagttctgga
     6241 cccgtgaccg gattgctcgg gtagccgact gggcgtggcc catcgtggag gctcactggc
     6301 gctccgtatt gcccggagaa cggctgttcc gcggcgtgga taggtgggag gctggcgact
     6361 ttcaccgcga gcgtctgcgg gcgctaggag tcccgcacca cacacttcac gacagccggc
     6421 accactgggc cgtcaggatg gtgcgcgccg gaatgccgct cgagctggtg gcacggtagc
     6481 tcggacacaa ggacgtggtg atggtcgcgc gggtgtatgg ccgattcgtg ccgaacagtc
     6541 aggagcgcga ccgctgggag catcgcggcg gcgttggacg cagaaaaatg gggtcggcaa
     6601 ggtgcactgg gtggtgcacc gaatgacaaa ccacttgaac cgagcggccc aagtggttgc
     6661 gacgatgata gcaggggagg gactcgaacc ctcgaccccg gcattatgag tgccgtgctc
     6721 taaccacctg agctaccctg ccgaacggac ggcaatccta ccccggccga ccgcaaagcg
     6781 tcaagcatca gtggccggca gccgagtcgg gccgaacgat cttgtagagg aactcgccct
     6841 tccggatcat tccgaacgac tcccgtgcga ctcgctcctg cacccgtggg tcccgctcga
     6901 tcgcgacggc cgcccgggtg agcgagtcga cgacttcctg gagccgggcc acctccgccg
     6961 cctcgctcgc gacctgactc tccagcttga acaggtcgcg ggtgctgtac tcgccgcctt
     7021 gcagtgcaaa taccaccgcc gccagcagca cagcgattgc ggcccagcga cccggcgtca
     7081 cagcgggtag agatctcttc ccggatagtg ggccagctcg cccagctcct cgccgatacg
     7141 aagcagctgg ttgtacttcg cgatgcggtc cgtgcggctg gcgctgccgg tcttgatctg
     7201 ccctgccccg ctggctaccg ccagatccgc aataaacgtg tcttcggttt cacccgaccg
     7261 atgcgagatc acgactccgt aggcgctgcc cttggccagc tcgatgcatt gcagcgtctc
     7321 ggtgagcgtg ccgatctgat tcaccttgat gagaatggcg ttggcgacgc cctcctcgat
     7381 cccgcggccc agtcggtcga cattggtgac gaacagatcg tctcccacga gctgaacccg
     7441 atcgtgcagt cgctcggtga gaagcgtcca gccctcccag tcgtcttcgg cgagtccatc
     7501 ctcgatcgag acgatgggat accggtctac ccactgcgtg tacaggtcca ccatttccgc
     7561 ggcactccga cgactcccgt ctcccttctt gaagacgtac tcgccgtcct gatgaaactc
     7621 tgacgcagcc gggtccaggg cgatcgccag atcccggccc ggctggtatc ctgctgcctc
     7681 gatggcctgc atgaccgcgt cgagcgccgc ctcgttggag ggcagcatcg gtgcgaaacc
     7741 gccctcgtcg cctaccgcgg tagagaggcc ctgtttgctc aggaccttct tgagcgcatg
     7801 aaaggtctcg acccccatgc gcaaggcctc actgaatgcc tcggccccga tgggcacgat
     7861 catgaactcc tgcacatcca cgttgttcga ggcgtgggcg cccccgttca ggatattcat
     7921 catcggcacc ggaagaacgc gtgccatcgg tccacccaga tagcggtaca gtggaagtcc
     7981 ggtgtcagac gctgcggcgc gcgcaaccgc gagcgacacg gcgaggatcg cattcgcgcc
     8041 gagcttgctc ttgttgggcg tgccgtccag gtccatcatc tcggcatcga ctgcgatctg
     8101 gtcgctcgca ctcattcctt cgagccgcgg tccgatcacc tcgttcacat tgcgaaccgc
     8161 ctcactgacg cccttgccgc cgtagcgctt ggtatctccg tcccgcagct cgacggcttc
     8221 gtgctctccg gtggacgctc cgctcggcac tgcggcgcga ccctgcgctc cactcgagag
     8281 gacgacatca gcttcgacgg tgggattccc gcggctgtcg attatctcgc gggcatgcac
     8341 ttcgatgatg gtggacatgc agctcctgga ggggcggaaa gacggcaagc tatggcgtgc
     8401 tccgagcagc gtcaacgtcg gcttgctggc gctggaacca ggtgcgagcg cgagccagca
     8461 aatcatcgcg atcgtcgtaa tccagccctt cagtcggtat cggatcaccc acgattaatg
     8521 caaccgggcg gactcgcagg cacaggctgc cgggcggcag cacccaatgg gtgccgccca
     8581 agtacaccgg aaccagaggg acgccggccg caatcgcgag cacgaacgct cccttcttga
     8641 attccccgag gcgcccgtca cggcttcggg ttccctcgac gaagatgatt accgaacggc
     8701 cgccccggat gatctccgcc gcattctcgt agctgtgggt cgccgcatcg aggtcccgcc
     8761 gatccaccgg gatctggccg gtggcgcgga gcccgcggcc gaagagcgga atggcgtaca
     8821 gctcacgctt ggcgatgaag cggagcgagc ccggcagact cgccagaaga atccagatat
     8881 ccacgaacga cgcatgattg gcagcgaata cataggggcg cccggcctcg agccgctcga
     8941 gtccgtccac cgagaccggc agccccgtag cggagatgag gcgccgagcc cagtcgcgag
     9001 gctcgcggtc gtagacaccg cccgcccggt ccgggacgcc gaccaatgct gccacactca
     9061 cgcgcagcgc atgccagagc gtgaagccca cgatacaggc cagcgaccag agcgcatgca
     9121 ttcagccaaa atgatcgaaa ccggtcagag tcacttcggc gcccttcagc aggagctgcg
     9181 tgccggttcc cgtgagcacc tggccgatac gggtcaacag caagccggtt tctttgagaa
     9241 aagcctgcgc tccggcatca tcgaacgacg ccggcaagct agctagcagc tcgtaatctt
     9301 cgccgcctcg agcagcaaat tctgccgggg atactcccgc tgcttgtgcg gactttccgg
     9361 ctgacgctgc gaccggcagt cggtccagct cgatccgcaa ctcgaccgcc gaggcggcgg
     9421 caaggtggcg ggcatcggag gcgaggccgt cgctgagatc gatcagtgcc gttgcacccc
     9481 gagcagccag agcacgcccg gccgcaattc ttggctcggg cctcccaaag gcccgccgcg
     9541 cttccggctc gggctccttt ccttgccgcc aagcctccac cgcggcacgc gctccgccca
     9601 gcatcccggt cacccagacg ccgtcgccgg ctttcgctcc ccgccgggag agaggccgag
     9661 ttgcgcggcc gactacggtg atcgcaaggc tccacgccgg cccgccggac agatcgccgc
     9721 ccagaaccac cccacccacg gaaccgacgg cctcgcccac accctgcatc aactcagcca
     9781 gctgcttctc cctggtatct cggggtacgg tcaccgccgc cagcaagccg atcgcctcgg
     9841 cccccgctgc agcgagatcg gacagagcag tcgcggcagc gcgccagccg atttcagctg
     9901 ggccgagcca ctccctgcgg aaatgaacct cttcgacgga aacgtcggtg ctgagaacga
     9961 gattcgagcc ggaaacctcc ggcaggatgg cgcaatcgtc gcccaggcct gccgcgagcg
    10021 ggcccagtgc ccgcgctatg gcgcggaccc ggtcgaactc gggacccggg cccaggggca
    10081 acatcgctat
//
LOCUS       DASRJQ010000042         7787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2223174, whole genome shotgun sequence.
ACCESSION   DASRJQ010000042 DASRJQ010000000
VERSION     DASRJQ010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7787
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2223174"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VFB89_01010"
     CDS             complement(<1..342)
                     /locus_tag="VFB89_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HZM25915.1"
                     /translation="MAARPRLLGLPYDASSSFLKGAAEAPPLIRAAIFSPHWNSWTEQ
                     FVDLAAADGLSDGGDLALTPDALARGNIEAGIEALLAEGWRPIALGGDHSVTYPVVRA
                     MSRAHTPLTVLH"
     gene            516..902
                     /locus_tag="VFB89_01015"
     CDS             516..902
                     /locus_tag="VFB89_01015"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZM25916.1"
                     /translation="MSSMVTFANPQLDQVYGAIADPTRRAILGLLSRGEVNIGSLADQ
                     FPISLNGVSKHVKVLERAGLVERSVRGREHWLRLQAEPLREAARWLDHYRAFWDSRLD
                     ALEMLLVRQQSTRTTGKKRRSRGSRS"
     gene            899..1348
                     /locus_tag="VFB89_01020"
     CDS             899..1348
                     /locus_tag="VFB89_01020"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HZM25917.1"
                     /translation="MTLPNDREDVLVVRRLLPVRREDVFAAWLDPVSLAQWMCPGDVE
                     RATVEVDPKVGGKFRIVMWRPRADGAEHWGEYLAIQPPSLLSFTWLSAYTDLTPTVVT
                     IELLERGASTELILTHRRLPAPRVDAHRQGWTDIVRKLEAVLTRHPT"
     gene            1352..2641
                     /locus_tag="VFB89_01025"
     CDS             1352..2641
                     /locus_tag="VFB89_01025"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HZM25918.1"
                     /translation="MIWNREVETLPRDRMDALQLERLRATVGRVLTGVAPLAERLRAA
                     GIRSPGDIASLADLGRLPFTRKIDLREHYPFGLFAVPKSSLARIQGSSGTRGKPTIVG
                     YTRNDLAVWAEVVARCLALGGVKPGMVIHNAYGYGLFTGGLGLHGGADLLGCTIVPIS
                     GGMTQRQAMLLRDLGGQVLCSTPSYALSIAEAIEAAQIPRSSLRLEIGIFGAEPWTDQ
                     LRSELEHRLGLTALNIYGLAEIIGPGVSAECIEGRNGSHVQEDHFLAEIVDPESGQAL
                     PPGREGELVFTTLTKEALPLIRYRTGDICSLDVTPCTCGRTTARMSRVRGRHDDMLII
                     RGVNLYPSEVERILLEVGDLGPHYQLIVERPGSLDELSILCEPAREGLDRDLLGSRLH
                     HALREQIGLSVTVLVVDREAVPRSEGKAIRVVDRRPR"
     gene            complement(2653..4701)
                     /locus_tag="VFB89_01030"
     CDS             complement(2653..4701)
                     /locus_tag="VFB89_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZM25919.1"
                     /translation="MHDVHEFLRSLTIVLGIAALTTVLFQRLRQPVVLGYIIAGLLIG
                     PHVPFPLVANGDIVRTLSELGVILLMFSLGLEFSLSKLVRVGPTAGVTAVIQSFLVAW
                     LGFLLGRVFGWSVLESVFVGAMIAISSTTIIAKAFDEQSIGGRLRELVVGVLLLEDLI
                     AVVFLAALTAIATGAGLSASALFPTIGRLAAFLAALLAVGILLVPRFIRFVVRLSRKE
                     TTLVASIGLCFATAYLAQEAGYSVALGAFIAGSLVAESGHSEEVEHLIEPLRDVFLAI
                     FFVSVGMLIDPAVAFQHWGAIAAFTVLVIVAKVITVSFGAFVGGTGIRTAVQAGMSMA
                     QIGEFSFIIAGVGLALGATRDFLYPIAVAVSAITTLMTPWLIRAAEPFAAFVDRKLPH
                     PLQTFATLYGAWLERLRRRPDTRRDARVTRIVRLLALDAVVLSALIALGAVELERVVG
                     LVSRAFGIAPDLARAILIAGGCVLVLPLLVGIGRLSRRLGAIIGATAIPPGGSKVDLD
                     AAPRHALVLTLQLTAALVTGVIVLVLTQAFLPPYVGVVVLTILLLGYGLAIWRGATTL
                     DSHVNAGAQAVVEALAKYARAGQTSVNDQAMEKVHRMLPGLGTPVALVLAEGGPAVGR
                     SLAELNLRGRTGATVLAISRNGQAIVAPGAGERLQAGDVLGVTGTEEAVAAAREMLGG
                     "
     gene            4851..5363
                     /locus_tag="VFB89_01035"
     CDS             4851..5363
                     /locus_tag="VFB89_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25920.1"
                     /translation="MVPTTGAVGGLAGLLEPWANFYSDHRWLQTTLIFVHLSGIFLGG
                     GFAIAADRDTFIAMQNARLSGQIRHLGKIRSIHKPIMLGLALALGSGFLLFLADVKEL
                     SGSAVFWVKMVLLGALLTNGYLLKHTEETMNAGDPDSPRLWNRLWKISLASMVLWLAV
                     ILAGTLLTAG"
     gene            5360..5887
                     /locus_tag="VFB89_01040"
     CDS             5360..5887
                     /locus_tag="VFB89_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HZM25921.1"
                     /translation="MMRRDSSSSTSCCSDCDETGGRRAFLRELAAIAAGVAAALEIRP
                     ERAAAWSVGFATGRTAFRDAVSYAIPSQDGVTIDKDREVILVRYQQAVYGFSLSCPHQ
                     KTPLRWQEDEHHFKCPKHKSRFEPDGTFIDGKATRNMDRYAIQRQGNDVVVDLGKLYR
                     EDENREGWIAAVVRL"
     gene            5884..6294
                     /locus_tag="VFB89_01045"
     CDS             5884..6294
                     /locus_tag="VFB89_01045"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZM25922.1"
                     /translation="MNRVFAGAVVLSLGVGPVDGAAQAAADRLQVSDEVYKGWKYFQV
                     YCSRCHGDDALGTMAAPDLTYSLSTEGGITADSFQVMVRKGSSDSSAAPDKRMKGFED
                     LLDPSLIDALYQYVKARSDSSLPPGRPHRAPASQ"
     gene            complement(6308..6775)
                     /gene="soxR"
                     /locus_tag="VFB89_01050"
     CDS             complement(6308..6775)
                     /gene="soxR"
                     /locus_tag="VFB89_01050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HZM25923.1"
                     /translation="MPKPIAPAPAAAPLLPISEVARRSGVAASALRFYEERGLIRSVR
                     SGSGHRRFPRAVLRRIAFIVFAQRIGLTLEEIGAELLRLPADRAPTGADWSRLSRTWT
                     SRIDARIAELERLRVGLTECIGCGCLSLDRCQLANPGDRAARLGPGPRYWIAD"
     gene            6839..7585
                     /locus_tag="VFB89_01055"
     CDS             6839..7585
                     /locus_tag="VFB89_01055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZM25924.1"
                     /translation="MTHHPDSLTGLRVAVTGGTSGLGLALVEALHARRAHVAFIARHA
                     DRVATVAQAHPGAHGIVGDVSRKSDTYPLALRITAALGGLDVLINNASSLGPVPLALL
                     GDTDCEDLERALATNLVGPFRLTKALLGALSASARAGRPALVINVISDAAVTAYPGWG
                     AYGASKAALLHLTRIWNQELEPHGIRLHAIDPGDMDTPLHALAVPDADRSTLKQPSGS
                     AREIIEYIEAWIQEGAEQHAGSRKADATPA"
     gene            7548..>7787
                     /locus_tag="VFB89_01060"
     CDS             7548..>7787
                     /locus_tag="VFB89_01060"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HZM25925.1"
                     /translation="MQAAEKPTQRPPDARLLVVEADGTMRHVQRRRLPACLRAGDLLV
                     ANDASTIPASLTGIHRSSGEPLEIRLAGRASLEFDD"
BASE COUNT         1399 a         2526 c         2577 g         1285 t
ORIGIN      
        1 gtgaaggacg gtgagcggcg tgtgcgctcg actcatcgct cggacaaccg ggtaggtcac
       61 cgaatgatcg ccccccagcg caatcggtct ccagccctca gcaagcagtg cctctattcc
      121 ggcttcgatg ttgccgcgtg ccagcgcgtc gggggtgagc gcgagatcgc cgccgtcact
      181 caggccgtcc gctgctgcga gatcgacgaa ctgctcggtc cagctgttcc agtgcggaga
      241 aaagatcgcc gcgcggatga gcggtggcgc ttccgccgct cccttcagga acgacgagct
      301 ggcatcatag ggaaggccga gtaggcgcgg ccgcgcagcc aaaggttcta ctccgcgtaa
      361 acgatcgtgg taccggaccc aatactagtc catacttcgc gcacagccac gtctctcgga
      421 ggatgggcgg ccgatccttg ctgctgctgc atccgacgga taccatgttg cctcctggcg
      481 tgtgccgtcg ctacatttat caaccactcg gttgaatgtc gagtatggtc acctttgcca
      541 acccccagct ggatcaggta tacggcgcga ttgccgatcc cacccgtcgc gccatcctgg
      601 gcctgctttc ccggggcgag gtaaatatcg gtagtctggc cgaccagttt cccatctcgc
      661 tcaatggcgt ctcgaagcac gtgaaggtcc tggagcgggc cggactggtc gagcggagcg
      721 ttcgcggccg ggagcactgg cttcggcttc aggccgagcc gctccgtgaa gcggcgcgat
      781 ggctcgacca ttaccgtgcg ttctgggaca gcaggctgga tgcgctcgaa atgttgctgg
      841 tgcgccagca aagcacgcgt accaccggca agaagcggcg ctcccgcgga tctcggtcgt
      901 gacgttgccg aacgacagag aggacgtgct ggtggtgcgc cggctgctgc ccgtccgacg
      961 cgaggacgtc tttgccgcct ggctcgatcc cgtcagcctg gcgcaatgga tgtgtccggg
     1021 ggacgtcgag cgggcgacgg tcgaggtcga ccccaaggta ggcggcaagt tccggatcgt
     1081 gatgtggcgg ccgcgcgcgg acggagcaga gcattggggc gagtatctcg cgatccagcc
     1141 gccatcgctg ctgtccttca cctggctctc ggcgtacact gacctgacgc ccacggttgt
     1201 cacaatcgag ctgctggagc gaggcgcctc gaccgagttg atcctcaccc accgtcggct
     1261 tccggccccg agagtggacg cgcatcggca ggggtggacc gatatcgtcc gcaagctcga
     1321 agcggtcctc acccgccatc cgacctgagg catgatctgg aatcgcgagg tcgagactct
     1381 tccgcgcgac cggatggatg cgcttcagct cgagcggctg cgcgccaccg tggggcgagt
     1441 gctgaccggc gtggcgccgc tcgcggagcg gctgagggca gcaggcatcc gttccccggg
     1501 cgacatcgct tctctggctg acctcggcag actgccgttt acccgcaaga tcgatctccg
     1561 cgagcactat cccttcggcc tgttcgctgt gccgaagtcg agcctggccc ggatccaggg
     1621 ttcgagcggt acccgcggaa agccgaccat cgtgggctac acgcggaacg acctggctgt
     1681 ctgggccgag gtggttgcgc gctgtctggc cctcggcgga gtcaagcccg gaatggtgat
     1741 tcataacgcg tacggctacg ggctgttcac cggcggcctt ggcctccacg gcggggccga
     1801 tctcctgggc tgtaccatcg tgccgatctc gggtggcatg acccagcgcc aggcaatgct
     1861 gctgcgcgat ctcggaggcc aggtgctctg cagcacccct tcctacgcgc tcagcatcgc
     1921 ggaggcaatc gaggccgcac agattccgcg gtcgagtctc aggctcgaga tcggaatttt
     1981 cggcgcggag ccctggaccg atcagttacg atccgagctg gagcatcggc tcggcctcac
     2041 cgctctcaac atctacggac tggcagagat cattggcccc ggcgtttccg cggagtgcat
     2101 agagggtcgc aacggatctc atgtacagga agaccatttc ctggcggaga tcgtcgatcc
     2161 ggagagcggg caggcgttgc cgccggggcg cgagggcgag ctggtcttca ccacgctcac
     2221 caaagaggcc cttccgttga tccgctatcg caccggcgat atctgctcac tcgacgtcac
     2281 tccctgcacc tgcggccgaa ccacggcacg aatgagccga gtgcgcggcc gccacgatga
     2341 catgctgatc atccggggag tgaacttgta tccgtcggag gtggagcgaa tcctgctcga
     2401 ggtcggggat cttggtccgc actaccagtt gattgtcgaa cggccgggga gtctggacga
     2461 gctgagcatc ctctgcgagc cggctcgtga gggcctggac cgcgatctgc tggggtcacg
     2521 tctgcatcac gcgttgcggg agcagattgg gctgtcggtt accgtcctgg tggtggatcg
     2581 ggaggcagtg ccgcggagcg agggcaaggc gatacgggtg gtggaccgcc ggccgcgata
     2641 ggccgcccgg actcatcctc ctaacatttc tctggccgcc gcgacagctt cttcggtacc
     2701 ggttacaccg agcacatcac ccgcctgcaa tcgctcgccg gcgccgggcg cgacaatcgc
     2761 ctgcccgttc cgtgaaatcg ccagcacggt cgctccggtt cgacctcgaa ggttgagctc
     2821 tgccaaactg cgtccgaccg ccggaccgcc ctcggccagc acgagcgcga ccggtgtgcc
     2881 cagccccggc agcatgcggt gcactttctc catcgcctgg tcgttcaccg acgtttgtcc
     2941 cgcgcgcgca tacttcgcga gcgcttccac caccgcctgt gcgccggcgt tgacgtggct
     3001 gtcgagggtg gtggcccctc gccagatcgc gaggccatag ccgagcagaa ggattgtcag
     3061 caccaccacc ccgacgtagg gcgggagaaa cgcctgggta agcacgagca cgatcacgcc
     3121 ggtaaccagc gccgccgtga gctgcagggt caggacgagc gcgtgacggg gcgcggcatc
     3181 gaggtccacc tttgagccgc caggtggaat cgcagtcgca ccgatgatgg cgcccagcct
     3241 gcgcgacaga cgcccgatgc caaccaggag cggcaggacc aggacgcagc ctccagcgat
     3301 caggatggcc cgcgcgaggt ccggcgcgat gccgaatgct cgactgacga ggccgaccac
     3361 acgttcgagc tccaccgcgc ccaacgcgat caaggcgctg agaaccaccg cgtcgagtgc
     3421 aaggagtcgg acgatccggg tcactcgggc atcgcgtcgg gtgtcgggtc tgcggcgcag
     3481 tcgctcgagc caggcgccgt aaagcgttgc gaacgtctga aggggatgcg ggagcttccg
     3541 atcgacgaag gccgcgaagg gttcggcagc gcggatgagc cacggcgtca tcagagtggt
     3601 aatggcggag accgctacgg cgatcgggta caggaagtcg cgggtggcgc cgagagcgag
     3661 gccgacgccg gcgatgataa aggaaaactc gccgatctgc gccatgctca tcccggcttg
     3721 caccgcggta cgaatgccgg tgccgccaac aaaggcgccg aaggagacgg tgatgacctt
     3781 ggcgacgatc acgaggacag tgaatgccgc gatcgccccc caatgctgga aggcgacggc
     3841 cggatcgatc agcatcccta ccgacacgaa gaaaatcgcg aggaagacat cgcggagcgg
     3901 ctcgatcaga tgctcgacct cctcactgtg tcccgactcg gcgacgagtg atcccgcgat
     3961 gaaggcgcca agggcaaccg agtagccggc ctcctgagcg agatacgccg tggcgaagca
     4021 gagcccgatg ctcgccacca gcgtggtctc cttgcggctc agccgcacca cgaaacggat
     4081 gaagcgcggg accagcagaa ttccaacggc gaggagtgcg gccagaaagg cggccagccg
     4141 cccgatggtc gggaaaaggg cgcttgcgga aaggcccgca ccggtcgcga tcgccgtcag
     4201 ggcggcgagg aacacgacgg cgatcaggtc ctccagcagc agcacgccga ccaccagctc
     4261 ccgcagccgg cctccgatgc tctgctcgtc gaatgccttc gcgatgatcg tagtgctgga
     4321 gatcgcgatc attgccccca cgaacacgct ctccagcaca ctccagccga aaacgcgccc
     4381 aagaagaaag ccgagccatg ccaccaggaa cgattggatg accgcggtga caccggcggt
     4441 gggcccgacc cgcaccagct tcgagaggct gaactcgaga ccgagcgaaa acatgaggag
     4501 gatgacgccc agctcggaga gggtccggac gatgtccccg ttggccacca acggaaacgg
     4561 gacgtgcggc ccgatcagga gtcccgcgat gatgtagccc agtaccaccg gctgccgcag
     4621 tcgctgaaag agcacggtgg tcagcgcagc gatgccgagt acgatggtca gggatcggag
     4681 aaactcgtgg acgtcgtgca tgctggtgcg gcgtcaggta gttgttttct taagatcgtc
     4741 aggtacctgc cgggtatgaa ggttgggggc cgaggtctac tctggcaggg aggcgtcccg
     4801 ggccgtaagt tgaccgcacc cagttacaac cctaacccgg agcgagccct atggtcccca
     4861 ccacgggggc agtcgggggt ctcgctggcc tgctcgaacc ttgggccaac ttctactccg
     4921 atcatcggtg gctccagacc accctcatct tcgtgcacct gtccggcatc ttcctcggcg
     4981 gggggtttgc catcgcagcg gatcgcgaca ctttcatcgc catgcagaac gcccgcctgt
     5041 cgggccagat ccgccattta ggaaagatcc gtagcatcca caaaccgatc atgctgggac
     5101 tggcgctcgc cttggggagc gggtttctcc tctttctggc tgacgtcaaa gagttgagcg
     5161 gttcggcggt attctgggtc aaaatggttc tgctcggggc gctcctcacc aacgggtatc
     5221 tcctcaagca caccgaggag acgatgaatg ccggcgaccc ggattcgccg aggctctgga
     5281 accgcctgtg gaagatttca ctagccagta tggtgctctg gctcgctgtg atcctggctg
     5341 ggacgttgtt gaccgccgga tgatgagacg cgactcgagc agttctactt cttgttgcag
     5401 cgactgcgac gaaaccggcg gccggcgagc gtttctgcgc gagctcgccg ccatagctgc
     5461 cggagtcgca gcggccctgg agatcagacc cgagcgcgcg gctgcatggt cggtcggctt
     5521 cgcaacgggc cggaccgcgt ttcgcgatgc agtgagctac gcgattccct cacaggacgg
     5581 cgtgaccatc gacaaagacc gtgaagtcat cctggtgcgg tatcagcagg cggtttacgg
     5641 gttctcgctg tcgtgccccc atcagaaaac tcctctacgc tggcaggaag acgagcatca
     5701 cttcaagtgc cccaagcaca agagcaggtt cgagccggat ggaacgttca tcgacggcaa
     5761 agcgaccagg aacatggatc gctatgccat tcagcggcag ggcaacgatg tggtggtgga
     5821 tctcggcaag ctctaccggg aagacgagaa tcgggaaggc tggattgcgg cagtggtgcg
     5881 gctgtgaacc gggtcttcgc cggtgctgtc gtcctgagcc tgggcgtggg tccggtagac
     5941 ggcgcggccc aggctgcggc cgatcgtctc caggtgtcgg acgaggtcta taaaggctgg
     6001 aagtatttcc aggtctattg ctcccgctgc cacggcgacg acgcgctcgg gaccatggcg
     6061 gcacccgatc tcacctattc tctcagcacc gagggcggca ttacggccga ctcgttccag
     6121 gtgatggtcc gaaagggttc ctccgactct tcggctgcgc cggacaagcg aatgaaggga
     6181 ttcgaggacc tgctggatcc atcgctgatc gacgcgctct accagtacgt caaggcccgg
     6241 agcgacagct cgctcccgcc cggcagaccg caccgagcac ccgcgtcgca gtgagggtgc
     6301 gcctggctca gtcggcgatc caatagcgag gcccggggcc cagccgagcg gcgcgatctc
     6361 ccggattggc gagctgacag cggtcgagag acaggcagcc gcacccgata cactcagtca
     6421 gaccaaccct caggcgttcc agctccgcga tgcgcgcgtc gatgcgcgag gtccaggtac
     6481 gcgacaagcg tgaccagtcg gctccggtgg gtgcgcggtc cgccggcagc cgtagcaact
     6541 cggcgccgat ctcctccaga gtgagcccga tccgctgcgc gaagacgatg aacgcaatcc
     6601 ggcgcagcac ggcccgggga aaacggcgat ggcccgagcc cgagcgaacg gagcggatga
     6661 ggccgcgctc ctcgtagaac cggagggcgg aggcggcgac cccgcttcgg cgggcgactt
     6721 cgctgatcgg caggagcggg gcggcggcgg gggcgggggc aatcggcttc ggcatgcttg
     6781 gaggatagcg gccccggccc ttgacttcaa gttcacttga acttgtagct tgcagcccat
     6841 gacacaccac cctgacagcc tgaccggcct tcgggtcgcc gtcaccggcg gcacctccgg
     6901 cctcggcctc gcgctcgtcg aggccctcca tgctcgccgc gcccacgtcg ccttcatcgc
     6961 gcgacacgcc gatcgggtgg ctacagtcgc ccaggcgcac cccggcgcgc acggcatcgt
     7021 gggagacgtc tcccggaaat ccgacaccta tccgcttgca ctccgcatca cggccgcact
     7081 cggtggcctc gacgtgctga tcaacaatgc ctcgagcctg ggcccggtcc cgctggcgct
     7141 cctgggcgac accgactgcg aggatctgga gcgcgctctt gcaaccaacc tggttggccc
     7201 ttttcgactt accaaagcac ttctcggggc gctcagcgct tcggcccggg cgggccgccc
     7261 ggcgctggtg atcaacgtga tcagcgacgc cgcggtgacc gcgtatcctg gttggggcgc
     7321 gtacggtgcg agcaaggccg ccctcctcca cctgactcgg atctggaacc aggagctcga
     7381 gccgcacggc attcgacttc atgcgatcga tcccggcgac atggatacgc ccctgcatgc
     7441 gctcgccgtt cctgacgcag accgaagcac actcaagcag ccgagtggct cggcccgcga
     7501 aatcatcgag tacatcgaag cctggattca ggaaggggca gagcagcatg caggcagccg
     7561 aaaagccgac gcaacgcccg cctgatgcca ggctgctcgt ggtggaggcc gacggcacga
     7621 tgcggcatgt gcagcgccgt cggctgccgg cctgtctccg cgctggcgat ctgctcgtcg
     7681 cgaatgacgc gtcaaccatc cccgccagcc tgacgggcat ccaccgatcg agcggcgaac
     7741 ccctggagat ccggctggcc ggacgggcgt cgctcgagtt cgacgat
//
LOCUS       DASRJQ010000043         2998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2776921, whole genome shotgun sequence.
ACCESSION   DASRJQ010000043 DASRJQ010000000
VERSION     DASRJQ010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2776921"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="VFB89_01065"
     CDS             <1..539
                     /locus_tag="VFB89_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZM25926.1"
                     /translation="QDRVATAAMEVMLGPVGAIIMAIAILISTFGCNNGLILAGARVY
                     YAMARDRLFFRQAGELHPDFRTPVFGLVAQALWTMVLCVSGTYSELLNYVIFAALVFY
                     LLTAVALFRLRRLRPDLLRPVKAVGYPVLPALYIAATGFILVVLLVDKQQRLYSGLGL
                     LIVLLGLPVYALWRRTAR"
     gene            536..2350
                     /locus_tag="VFB89_01070"
     CDS             536..2350
                     /locus_tag="VFB89_01070"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HZM25927.1"
                     /translation="MKHQRLTFGICGLVWLAVLACGGSSAPSAPTPKTSTPATPVTGL
                     ADELFAGFLQRSPESATVFGVANGPHDRLTDLSPGARNTWEAREDAWLARLQAIDTSK
                     VIGTPDAPVYAIMRQTLEANRASRVCRLELWNVSQLFGWQVNLPRLTRLQRLGTPEAR
                     AQALARWQAIPRFIDQDIGNLREGLREGYTAPRVNVEEVLKQVDALLADPPEKSPFAG
                     FIERDSTPEFRDAIVGVVRDRINPALRRYRSFLADEYMGRARTTPGVSANPNGAECYR
                     ARLQFYTTLPLDPREVHELGLSQMKRIEAEMQTIAQRSFGTTDVPALLERLRTDSQYT
                     FRTRQEVIAVAESSIVRAQAVMPKWFGKLPKAPVIVDPCQPFEEASGCPGSYVPAAED
                     GSRPARYRINAGDPTHKPRASAEAIAFHETIPGHHLQIAIAQERQGVHPITRFFFNSG
                     FVEGWALYAERIADEMGLYTSDLDRMGLLSNDALRAARLVVDAGIHALGWSRQQAIDY
                     MMRHTAQSQGFVESEVDRYIITPGQATAYMVGRLEIQRLRAQAEQRLGKRFDIREFHD
                     HVLQDGSIPLGLLRSNIERWLAEAPATAAAAEPATPWP"
BASE COUNT          500 a          984 c          947 g          567 t
ORIGIN      
        1 ctcaggaccg cgtcgccacg gcggccatgg aggtcatgct gggcccggta ggcgccatca
       61 tcatggcgat cgccattctc atttcgacct tcggctgcaa caacggtctc attctggcgg
      121 gcgcgcgagt ctattacgcg atggcacggg accgcttgtt cttccggcag gctggtgagc
      181 tgcacccgga ctttcgcaca ccggtcttcg gcctcgttgc gcaggctctc tggaccatgg
      241 tgctgtgtgt gagcggcacc tacagcgagc tgctcaacta cgtgattttc gcggctctgg
      301 tgttctatct cctgaccgcg gtggcgctct tccggctgcg ccggcttcgg cctgatctcc
      361 tgcggccggt caaagccgtt ggttacccgg tgttgccggc gctctacatt gccgcgaccg
      421 ggttcattct cgttgtgctg ctggtggaca agcagcagcg cctctattcc ggcctcgggc
      481 tcctgatcgt gctgctcggc ctgccggtgt acgctctctg gaggcgaaca gctcgatgaa
      541 gcaccaacgt ctgacctttg gtatatgcgg tctggtctgg ctcgccgtcc tggcctgcgg
      601 tggttcgtca gcgccatccg caccgacgcc caagacatca acaccggcaa ctccggtaac
      661 cgggctcgcg gacgagctct tcgccggatt tctgcagcgc tcccccgagt cggccacggt
      721 cttcggcgtg gccaacgggc cgcacgatag actcaccgac ttgtcgcccg gcgcccgaaa
      781 cacctgggaa gctcgtgagg atgcctggct ggctcgcctc caggctattg atacctcgaa
      841 ggtgatcggc acgcccgacg cgccggtcta tgcgatcatg cggcagacac tcgaggccaa
      901 ccgggcttcg cgagtctgtc ggcttgagct gtggaacgtg agtcagctgt ttggctggca
      961 ggtgaacctt ccgcggctta cccggctaca acggctcggt acaccggaag cgcgggcgca
     1021 ggcgctggcg cgctggcagg caatcccccg cttcatcgat caggacatcg gcaaccttcg
     1081 cgaggggctt cgcgagggtt acacggcccc gcgggtcaac gtcgaagaag tgctcaagca
     1141 ggtagacgcg ctgctggctg atccgcccga aaagtcgcct tttgccggat tcatcgaacg
     1201 ggattcgacg cctgagttcc gcgacgcgat cgtcggcgtg gtgcgcgatc gcatcaatcc
     1261 ggcactccgc cggtatcgca gtttcctggc cgacgagtac atggggcgtg cgcgcacgac
     1321 gcccggtgtt tcggccaacc cgaacggggc tgaatgctac cgggcccggc tccagttcta
     1381 caccacgctc ccgctggacc cccgcgaggt gcacgagctg ggactctcgc agatgaagcg
     1441 catcgaagcc gagatgcaga cgatcgcaca gcggagcttc ggtaccaccg atgtcccggc
     1501 actattggag cggctccgca ccgactcgca atacaccttc cgcactcggc aggaggtcat
     1561 tgctgtcgcc gaaagctcga ttgtgcgggc ccaggcggtg atgcccaaat ggttcgggaa
     1621 gcttcccaaa gcacccgtga tcgtcgatcc ttgccaaccc ttcgaggagg cctccggttg
     1681 ccccggctcg tacgttccgg ctgcagagga cggaagccgg ccggcccgat atcggatcaa
     1741 cgcgggcgat ccgactcaca agccgcgggc gagcgctgag gcaatcgcgt ttcacgagac
     1801 aatcccagga caccatctcc agatcgcgat cgcccaggag cgccagggag ttcatccgat
     1861 cacccgcttt ttcttcaaca gcggcttcgt cgaagggtgg gcgctctacg ccgagcgcat
     1921 agccgatgag atgggtctgt atacatccga cctggatcgc atgggcctcc tctccaatga
     1981 cgcgctgcgg gccgcccgcc tcgtggtcga cgccgggatt cacgccctcg gctggagtcg
     2041 ccagcaggcg atcgactaca tgatgcggca cacggcgcag tcgcaaggct tcgtcgagtc
     2101 cgaagtcgat cggtacatca tcacgcccgg acaggccacc gcctacatgg tcgggcggct
     2161 cgagattcaa cggctccgcg cgcaggccga gcagcgcctg ggcaagcggt tcgacatccg
     2221 ggagtttcac gatcatgtgc tgcaagacgg cagtattcct ttgggcctct tgcggagtaa
     2281 tatcgagcgg tggctcgcgg aggcccctgc gacggccgct gccgccgagc cggcgacgcc
     2341 atggccgtag ccttgtttct cttacgcaac cctgagggaa gagcgggtgt cagagcacca
     2401 tgacgcccgc tcttctcgtg ctcgctatca aaatcacttc cgctgcgtcc gccgggtcgg
     2461 gggatctcac gctggcccaa ttgctggcct gccacgatag tgccgtcggc ggcccggcgc
     2521 ttcgtgccgt gcaacgccag gaataccgga tctcgatcga ggaaggcggt gcgacgctcc
     2581 gaggccgata ccgcgcgacc cgcgacggcc gtacgcgcat cgatgtgttc gccgatagca
     2641 ttcgagtctt ttccgaaggc tgggacgggc agcacggatg ggaactggcc cagggagcgg
     2701 cggcgcccac gccaaccagc tcgccggcca ctgcgactct ctggcacggc ctcgctcaac
     2761 cggggcatct ctggaccctc gcggacatga agcggaatgg gcatgccgtc gcgcttgccg
     2821 gccgagagac gattgaggcc actagctact tacgcggtca tgctaacgct ttccgacggc
     2881 tttcggatct ggtacctgct cgatccgacg acgtgtctaa tcgtgcggaa gcgcgatttt
     2941 cgggcgtttc atcccaatct ggatcgccag cagaaatgga tcgagtcggt gttcgatg
//
LOCUS       DASRJQ010000044         2727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3357445, whole genome shotgun sequence.
ACCESSION   DASRJQ010000044 DASRJQ010000000
VERSION     DASRJQ010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3357445"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="VFB89_01075"
     CDS             <1..748
                     /locus_tag="VFB89_01075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZM25928.1"
                     /translation="NQALVVARRNAERAERLSQAGAMAERDLEQARWTVMNAEAGLAD
                     VSARLSTAEKQQGKTVVRAPFAGVVSERPVNTGDVVQVGNPVATVVDPRSLRLEATVP
                     VSALEVLKVGTTVDFEVSGYEGKRYVGRVERINPMLDPATRQVRIIVGIPNVTGRLVA
                     GLFAQGRVAIDQRTALAVPLSALDLKTPTPSVRRLRAGRVELVAVELGMQDDVMQVIE
                     IKAGLAAGDTVLVGPSAGVAAGTPVRIVKE"
     gene            755..>2727
                     /locus_tag="VFB89_01080"
     CDS             755..>2727
                     /locus_tag="VFB89_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZM25929.1"
                     /translation="MWISDSAIRRPVITVVVMVALVMFGLIALVTLKTDEYPDVAPPY
                     VNVGVPYPGASPETVEKEVLDPIEEAISSITGVKKVNGKAMDGFASIMIEFEFTKPLA
                     EATQDIRDGISGIRNDLPPEMEEPIIRKLNDTDRPIISLALSSAVLAPRELTRIADPG
                     ITRELRSIPGVAEVSVSGKQERELTVELKPDALQSAGISVGQVVLALQTQNLAAPVGR
                     LDGPLDERSIRLKGRLSGPAEFAQLVVAERGGRLIRLGDLATVKDGAEDPRTMALYNE
                     RVAVGIDIKKAKGYSTTEVSEAVMVRLARVEKTLPANVKLDLIRDAAVRVRHSVRDVQ
                     LTLFEGALLTVLVVFLFLNSWRSTVITGLALPVSVLASFIAVWIFGFTLNTMSLLGLS
                     LAIGILIDDAIVVRENIVRHVEMGKDHVTASHEGTDEIGVAVTATTLSILAVFVPIAF
                     LPGLSGQWFKPFGLTMACAVAVSLLVSFSLDPMLSAYWPDPHRTEAKKSWITRKLDRF
                     NEWFGRLAQQYKRVIAWALDHRLAVVALSLGTFVVALMMPRFGLVGVSFVPIDDKSEF
                     TVQLETPPGSNLEYTRRKAEEVGTLIRAHPEILYTYATLGGTQTGAVDEGQIYVRMKP
                     KHDRKLSAEALAGLIRQEIRSVVGVKLAVFTSD"
BASE COUNT          456 a          877 c          948 g          446 t
ORIGIN      
        1 gaatcaggcg ctggtggtgg cgcggcgcaa cgcggagcgg gcggagcgcc tgtcgcaggc
       61 cggcgccatg gcggagcgcg acctggagca ggcgcgctgg accgtcatga acgccgaagc
      121 cggactcgcc gatgtgagcg cacgcctgtc caccgcggaa aagcagcagg gcaaaaccgt
      181 ggtgcgcgcc ccgttcgccg gggtggtcag cgagcggccg gtcaacaccg gggacgtggt
      241 gcaggtgggc aatccggtgg cgaccgtcgt ggatccgcgg agcctccggc tcgaagcgac
      301 ggttcccgtg agtgcgctcg aggtgctcaa ggtcggcacc accgtcgatt tcgaagtgag
      361 cggctacgag ggcaagcggt acgtcgggcg ggtcgagcgc atcaatccga tgctggatcc
      421 cgccacgcgc caggtccgga tcattgtcgg gattcccaac gtaacggggc gtctggtggc
      481 cgggctcttc gcccagggcc gggtcgcgat cgaccagcga accgcgctcg cggttccgct
      541 gtcggcgctg gacctcaaga ccccgacccc gtcggtccgc cggctgcgag ccggaagggt
      601 cgagctggtg gcggtcgagc tcggcatgca ggatgacgtg atgcaggtga tcgagatcaa
      661 ggccggtctc gcggcgggcg acaccgtgct ggtcggcccc tcggcgggcg tcgcggcagg
      721 cacgccggtc cgtatcgtga aggagtgagg cgccatgtgg atttccgata gcgccatccg
      781 ccgtccggtc atcaccgtcg tggtgatggt cgcgctggtg atgttcggcc tgatcgcgct
      841 ggtcaccctc aagaccgacg agtatcccga cgtggcaccg ccgtacgtga acgtcggcgt
      901 gccgtatccc ggcgcgtccc cggagaccgt ggaaaaggaa gtgctggacc cgatcgagga
      961 ggcgatctcc tcgatcaccg gcgtgaaaaa ggtgaacggc aaggcgatgg acggcttcgc
     1021 gtccatcatg atcgaattcg aatttaccaa gccgctggcc gaggcgacgc aggacatccg
     1081 cgatggcatc tccggaatcc ggaacgacct ccctcccgag atggaggagc cgatcatccg
     1141 gaagctcaac gacaccgacc ggccgatcat ctcgctcgcc ctgtcgtcgg cggtcctggc
     1201 tccgcgcgag ctcacccgga tcgcggatcc cggcatcacc cgggagctgc gctcgattcc
     1261 gggcgtggcc gaagtgagcg tgtccggcaa acaggagcgc gagctcacgg tcgagctcaa
     1321 gcccgatgcg ctgcaatcgg cgggcatcag cgtgggacag gtggtgctgg cactgcagac
     1381 ccagaacctg gcggccccgg tcgggcggct cgacggtccg ctggacgagc gctcgatccg
     1441 cctcaaggga cggctcagcg gcccggcgga gtttgcccag ctggtggtgg ccgagcgagg
     1501 cggccggctg atccggctcg gcgatctggc gacggtcaag gacggcgccg aagacccccg
     1561 cacgatggcg ctgtacaacg agcgggtcgc cgtgggaatc gacatcaaga aggccaaggg
     1621 gtacagcacc accgaagtga gtgaggcggt catggtgcgg ctcgcgaggg tcgagaagac
     1681 cctgccggcc aacgtcaaac tggacctgat ccgtgacgcg gcggtccggg tgaggcactc
     1741 ggttcgggat gtacagctga ccctcttcga gggcgcactg ctcaccgtgc tggtggtgtt
     1801 cctgttcctc aactcctggc gctccaccgt cattaccggt ctcgcgctcc cggtctcggt
     1861 gctcgcctcc ttcatcgcgg tatggatctt cggcttcacg ctcaacacca tgtcgctgct
     1921 cggtctgtcg ctggcgatcg gaatcctgat cgacgacgcc atcgtggtcc gggaaaacat
     1981 tgtgcggcac gtcgagatgg gcaaagatca cgtcaccgcg tcgcacgagg gaaccgacga
     2041 gatcggcgtc gcggtgacgg ccacgacgct ctcgattctc gcagtgttcg ttcccattgc
     2101 ctttctcccg ggactttccg gccaatggtt caagccgttc ggcctgacca tggcgtgcgc
     2161 ggtcgcggtg tcgctgctgg tctcgttctc gctcgacccg atgctgtcgg cctactggcc
     2221 cgatccgcac cggaccgagg cgaagaagtc ctggatcacc cggaagctcg accggttcaa
     2281 tgaatggttc ggccggctgg cccagcagta caagcgcgtc atcgcctggg cactcgatca
     2341 ccggctggcg gtagtagcgc tctcgctcgg caccttcgtc gtggcgctga tgatgccccg
     2401 gttcggcctg gtgggcgtga gcttcgtgcc gatcgacgac aaatcggaat tcaccgtcca
     2461 gctcgagacg ccgccgggct cgaacctgga gtacacccgg cgcaaggccg aagaagtggg
     2521 gaccctgatc cgggcccacc ccgagattct ctacacctac gccacgctgg gcgggaccca
     2581 gaccggcgcc gtggacgagg ggcagatcta cgtccgcatg aagcccaagc acgaccggaa
     2641 gctttcggcg gaagcgctcg ccggactcat ccgtcaggag atcaggagcg tcgtgggcgt
     2701 caagctggcg gtgtttacca gcgacat
//
LOCUS       DASRJQ010000045         3528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3067076, whole genome shotgun sequence.
ACCESSION   DASRJQ010000045 DASRJQ010000000
VERSION     DASRJQ010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3067076"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="VFB89_01085"
     CDS             complement(<1..980)
                     /locus_tag="VFB89_01085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HZM25930.1"
                     /translation="MTTLQSPPGEVASVEAIRAHFPALERHHGGYPVAYFDGPGGTQV
                     PRQVADAMSDYLLHHNANTHWVYPTSQETDELIAEARSAVADFLNGAADEVAFGANMT
                     TLTFHLARGLGRQWSPGDEVIVTELDHHANVAPWRRLEQERGITIRTVPLRTETGQLD
                     WPALERAIGTRTRLLAIGGASNALGTVNDLAAAATLARNAGALTFVDAVHYAPHVLPD
                     VGSLGADFMVWSAYKMYGPHLGVLWGRRELLEGVDIPKLDPAPSAAPEKLETGTQNHE
                     AIAGLKAAIDFLAGLAPAVGSRRERLEATFAALHRRGEALLRRMWEGLGDV"
     gene            complement(1131..1494)
                     /gene="ssrA"
                     /locus_tag="VFB89_01090"
     tmRNA           complement(1131..1494)
                     /gene="ssrA"
                     /locus_tag="VFB89_01090"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1541..2974)
                     /locus_tag="VFB89_01095"
     CDS             complement(1541..2974)
                     /locus_tag="VFB89_01095"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZM25931.1"
                     /translation="MIEFAAGAALGALGAWAGSRLVAESGSRSGKRIRELGPHLLPEP
                     ALEWLLRANGALGIWINEIAPGEEGPRAERLIESEHLSVTQIAAVDRRLERARDQEQG
                     GAERLETGTLIFRAGGGAAVGMLLPQGRPAAALTDAERDLDRLLDAVRRRPQIVALAQ
                     AQTQEASLESVGSVGLRLAYQLERSLDAEVVVAAAEERVVRVVGVSGRADRRLLDSVL
                     APFSDLARVALGELAQTLTGGDPLGGAVNDRRQRQGASLLIPILVGSDPNPIGAVAVY
                     LPTAAEPAGPARAELMEALANAGPRLARALAGERANRQAKTDTLTGLANRRALDEAMR
                     QLAVEEGALIYADLDRFKQLNDSLGHAAGDAALVHFARIIREQVRAGDTAARIGGEEF
                     AVWLPRASLALGMRIAERIRIKVSTTPWDWSSKRWPLSASFGVAACPETNTNIENLAG
                     QADAALYVAKTNGRNRVEAAGAGAPPT"
     gene            complement(2971..>3528)
                     /gene="larB"
                     /locus_tag="VFB89_01100"
     CDS             complement(2971..>3528)
                     /gene="larB"
                     /locus_tag="VFB89_01100"
                     /inference="COORDINATES: protein motif:HMM:NF033503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HZM25932.1"
                     /translation="VVAIAERLAAVDGAFLGTRATAEMAEELGKQFTGVQWNASARTI
                     YLPPAKRAAAIGTALVISAGTSDLPVAEEAAVTLEAFGHTAARLCDVGVAGIHRLLSA
                     TDRLQAADVAIVVAGMEGALPSVVGGLVRVPVIAVPTSVGYGASFGGLAALLAMLNSC
                     AAGVTVVNIDNGFGAAAAASRILSA"
BASE COUNT          591 a         1226 c         1129 g          582 t
ORIGIN      
        1 acgtcgccaa gaccctccca catccgacgc agcaacgcct cgccccgcct gtgcaaggct
       61 gcgaaggtcg cctccaggcg ctcgcggcgg ctccccacgg caggcgccaa cccggccaga
      121 aagtcgatgg ccgccttgag cccggcgatc gcttcgtggt tttgggttcc ggtctcgagc
      181 ttctccggag cggctgacgg cgccggatcg agcttcggaa tatcgacccc ttcgagcagc
      241 tcgcgccgcc cccacagcac acctagatgg ggaccgtaca tcttgtacgc cgaccagacc
      301 atgaaatccg cccccagcga gccaacgtca ggcagtacgt gaggggcgta atggacggca
      361 tccacgaacg tgagtgcgcc cgcattccgg gcgagcgtcg ccgccgcagc caggtcgttc
      421 accgtcccca aggcattcga ggctcctcca atggccagaa gccgcgtgcg cgtgccgatc
      481 gcccgctcga gcgcgggcca gtcgagctga ccggtttcgg tccgcaaagg aaccgttcgg
      541 atggtgattc cacgctcctg ctcgagccga cgccacgggg cgacattggc atggtgatcg
      601 agctcggtca cgatgacctc gtcgcctggg ctccactgac gccccaaccc gcgcgccagg
      661 tgaaacgtca gcgtggtcat gttcgcgccg aaggcgactt cgtctgctgc gccattgaga
      721 aagtcggcga ccgctgagcg agcctccgcg atcaactcgt cggtctcctg gctggtcggg
      781 tagacccaat gggtgttggc gttgtggtgc aggagatagt cggacatggc gtccgccacc
      841 tgtcgcggca cctgagttcc accaggtcca tcgaagtagg caaccgggta tccgccgtgg
      901 tgccgctcga gcgcagggaa gtgagcgcga atggcttcga ccgaagcgac ctcgcctgga
      961 gggctctgga gcgtggtcat gggcgcctca aatcggttga gaatgggctg ataagtaagg
     1021 tcattacgta tgcactaagg cacattacca catattgtta ggagacattt cgcagcgtta
     1081 gttgccgcca agaaaaaacg ggacgtccga ccggacgtcc cgccgagacc tgatggaggc
     1141 gcagggaatc gaaccctggt ccgagtgagg ctccagaacc gcttctacgt gcgtaggctc
     1201 gatttcaacc ctgtctcgct cgaccggcat cgggccggcc tgccgagcga ccaccctcgc
     1261 tggctctcgc tcgaggcggt gagggaccac ctctcgccag tccggctttg cgatccgacg
     1321 gcagcgcccc ggacgggctc tgacgccgga ggtgcgttag cgagctgagc tcagcttacg
     1381 cagccagtgc cagttcagtg ttggcagtta aaacattcca gacggttttt ccaggaatct
     1441 ggacctgggc acgctacgat tccttcatcg cacccgtcga agccagtcgc ccccgggtct
     1501 tccaatataa ccagcgagag aaggagcccc cgtcggcggg tcaggtcggc ggcgccccgg
     1561 ctcccgccgc ctcgacccgg tttcggccat tcgtctttgc gacataaagc gctgcgtccg
     1621 cctggcctgc caggttctcg atgttggtgt tcgtttccgg acaggcggcg acgccgaagg
     1681 acgcactgag cggccagcgc ttggagctcc aatcccaggg ggtggtgctc accttgatgc
     1741 gaatccgctc ggcaatccgc atcccgagcg cgagggatgc ccgtggcagc cataccgcga
     1801 actcttctcc cccgattcgg gctgccgtat cgccggcccg gacctgctct cgaatgatcc
     1861 gggcgaagtg caccagagcg gcgtcgcccg cggcgtggcc caacgaatcg ttgagctgct
     1921 tgaagcgatc gaggtccgcg tagatcaggg cgccttcttc aacagcgagt tggcgcatgg
     1981 cttcgtccag agccctccga tttgcgagcc cagtcagggt gtcggtcttg gcctggcggt
     2041 tggcccgctc gcctgccagc gccctagcga gccgtggccc cgcgttggcc agcgcttcca
     2101 tcaactcagc ccgagccggg cctgccggct cggctgcagt cggcaggtag acggcaacgg
     2161 ctccgatggg gttcggatcg ctgcccacga ggatcgggat gagcagcgag gcgccctgtc
     2221 gctgccgccg atcgttcact gctccgccga gcgggtcgcc cccggtgagc gtctgcgcca
     2281 gctctcccag cgctacgcgg gcaagatcgc tgaatggggc cagcacggag tcgagcagac
     2341 ggcggtcggc ccggcctgag acaccgacga cccgcacgac ccgctcctct gccgcagcaa
     2401 ccacgacttc cgcgtccagg ctccgctcga gctgataggc gaggcgaagg cccacgctcc
     2461 cgaccgattc gagcgaagcc tcctgcgttt gcgcctgcgc cagggcgacg atctgggggc
     2521 gacggcgtac cgcgtcgagc agtcggtcga gatcgcgctc tgcgtccgtg agcgcggcag
     2581 cgggacggcc ctggggcaac agcatgccga ccgctgctcc tccgccggca cggaaaatca
     2641 gcgtccccgt ctccaatcgc tctgccccgc cctgctcctg atcgcgcgcc cgctcgaggc
     2701 gtcgatccac ggcggcgatc tgcgtgaccg agagatgctc ggattctatc agacgctcgg
     2761 cccgaggccc ctcctcaccg ggcgcaatct cgttgatcca gattcccaat gccccgttgg
     2821 ctcggagcaa ccattcgagc gcgggttcag ggaggaggtg cgggcccagc tcgcgaatcc
     2881 gcttccccga gcggctcccg ctctcggcga ccagtctcga gccggcccag gcgcccagcg
     2941 caccgagcgc ggcaccggcg gcaaactcga tcacgccgag aggattcggc tcgctgccgc
     3001 ggccgctcca aaaccgttgt cgatgttcac cacggtcacg ccggctgcgc aggaattgag
     3061 catggccagc agggcggcca ggccgccgaa cgaggctcca tatcccacgc tggtcggcac
     3121 agcaatgacg ggaacccgga ccaagccccc gaccacgctg ggaagggcac cctccattcc
     3181 ggcgacgacg atggcgacat cggcggcctg caaccggtcc gtggcggaga gcaacctatg
     3241 gattcccgca acgcccacgt cgcagaggcg ggccgccgtg tggccgaagg cttcgagcgt
     3301 gactgccgct tcttccgcta ccggcaggtc gctcgttccc gccgatatca ccagggcggt
     3361 gccgatggca gcggcgcgct tggcgggagg caagtagatc gttcgagccg atgcattcca
     3421 ttgaacccct gtgaactgct tacccagctc ttccgccatc tcggccgtgg cgcgagttcc
     3481 cagaaatgcg ccgtccacgg cggccagtcg ctccgcgatc gccaccac
//
LOCUS       DASRJQ010000046         4606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_250255, whole genome shotgun sequence.
ACCESSION   DASRJQ010000046 DASRJQ010000000
VERSION     DASRJQ010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250255"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VFB89_01105"
     CDS             complement(<1..526)
                     /locus_tag="VFB89_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25933.1"
                     /translation="MGHWAKLPSVAARPWLVMSGLSILGAVSCLYATHRGPGLFPDSA
                     TYLEGARNLLGGRGFVEITALGTSDPITDYPPLYSFVLAGLAYVGIDPEIGARWLQAG
                     LFGATVFLGSLAIRHLTSGSARAMVVGGVVMLTSVDLLGVFAQVGSEALFITLGLGAL
                     LLLGTHAERPDRVRL"
     gene            1722..2519
                     /locus_tag="VFB89_01110"
     CDS             1722..2519
                     /locus_tag="VFB89_01110"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZM25934.1"
                     /translation="MEVWSAGLQGGLLSLARDPTVGLRRLIFPVSYWRTPEFAYVRRE
                     LRLPNGASVLDLGSAKELGTWLALRRGYAVTSADILTAEVSRTERYASALGIRGSSPG
                     HLRAEVQDGRSLTFANDAFDAAYCVSTLGNIPGDGDSQTIRELLRVVRVGGVVVVTVP
                     FGSKYSETYVNRPVHERDFEGQPIFWERRYDRQSMVDRLQNLPGARLVSLEFWGERVR
                     LERWITGRGPLRFVLLPLEPLLALLALRPLADSDRATVAFFTLQKTS"
     gene            2939..3199
                     /locus_tag="VFB89_01115"
     CDS             2939..3199
                     /locus_tag="VFB89_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25935.1"
                     /translation="MEDIVFGIARDVQDWESWVTLTDEAAQGTPIGFRHHYIDYSQIK
                     PRVSPLEDAQRIPGIADLNRYKSRLSEHSRNYDTRHFLVVHY"
     gene            complement(3476..>4606)
                     /locus_tag="VFB89_01120"
     CDS             complement(3476..>4606)
                     /locus_tag="VFB89_01120"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZM25936.1"
                     /translation="TPPVKVQVQDVAGNPVTSAIDTITVTLGNAPNTASLAGVTSVKA
                     ANGTATFSDLSLGTGGTGYTLTAQGRGLVRATSAAFNVVTGESATAITGITPASSVVG
                     QPYDVTFTVGAVAPASGTPSGTVSVSDGTGATCTTSAPSGSCALTSTTAGTKSVVATY
                     VGDANFAASTSKATSHQVIAAATTVMITNAPATAFFGQPITVQFTVTVNPPGAGTPVG
                     TVTVTYGNSGGCTAPVTAGKCSFTPTAAGTGSLQAVFTPSTADFVTDQSPTVSYTVNR
                     ATTTTAVSSSKNPADEGESVTFTAVVAVANGQGTPTGTVAFRDGDATLATVPLSGNGS
                     ATTSSSFTAGQHNITAQYSGDSNFAGSTSAVLEQQVNTPASP"
BASE COUNT          854 a         1432 c         1461 g          859 t
ORIGIN      
        1 cgaggcgcac gcggtccgga cgctccgcat gcgttccgag caagagtaac gcgccgagtc
       61 ccaaggtgat gaacaaggcc tccgagccca cctgagcgaa gacgccgagc aggtccaccg
      121 atgtgagcat gacgacgcct ccgaccacca tggctcgggc ggaccctgat gtgagatggc
      181 gaatcgcgag gctgcctagg aaaaccgtcg cgccaaagag tccggcttga agccagcgag
      241 ctcctatttc agggtctatg ccgacatacg cgagaccagc gaggacgaac gagtacagcg
      301 gggggtagtc agtaatggga tcgctagtac cgagcgcggt tatctccacg aagccccgtc
      361 ccccgagcag gttgcgcgca ccctcgaggt aagtggcgga atcggggaac agccctgggc
      421 cgcgatgggt cgcgtacaga caactcacgg cgccaagaat ggagaggccg ctcatgacca
      481 accagggtcg agcggcgacg ctcggcagct tggcccaatg tcccaccgtt tcagggtcca
      541 cttttccggg gcaacatcat aaggctgcaa gcacgggtta ggctgtatcc atgccatgac
      601 atctcttgaa gcgcttcccg gaaccgcacg ggcaaggcgc attgcgggat gcgccactgt
      661 aatcaggttt ggtgtgtcct gccttggata cgaacacggg ccttcccgca tcaagggact
      721 gccgcaggtc cattaggacc ggcttcgtga ccttgatgct cggtgccggg ccgccgctcc
      781 gcaggtgtcg atatagctca tcaaagaggt ctgagtagct gggattcagg gtaaggaggt
      841 cggcgctcgt gtcaatcgct tcggtgagcc caccatgacg gatgaagtag ccagcgtact
      901 ctagctcgtc agtgccgtat accttcccgt ggcagcgagc ccggtcgcgc aggtagacca
      961 ctaactcggc cgcaccccat cccaaatact gccaggcctc acctaaagcg ccaaggtcga
     1021 atgcattcgt aacccacggg tagggcgcat cgccatgctt ctcgaggaga atcgtgaggt
     1081 cggttgcaag cgggccgaag ttggtctctg gtcacgcaaa cagagaacac atcgctgcac
     1141 tgtgacgggt caactgttcg aactagggcg ccgtttctgg agtacagcgg gatcggttcc
     1201 cccaatgcaa accgagcccg caggcgttcg gcctggtcat aggcagactg tattcccgat
     1261 tccgagccaa aggcgtgcgt taggcgaaca aaggtcttgt caggatcgcg aaatggagca
     1321 atcggaggag tcgcctttgc ttcgacgatg agggcggcct ttcctgagaa ggccgcaaat
     1381 tgcgctcgaa gtggccggtc ggagtttcat agacccctgt caccgctctg agcaaagcag
     1441 cttcgcgaat tggacggggc tggcgatggc tggcccccac cgtactttct ctcggcccgg
     1501 acgaacaagt ggagccgatg tgaaggatga tcgaggtgtg aggcgaccac cgcattcccg
     1561 aaccgagagc cgcgtcccca ccgcccctca tccgggagtg cctccgtttg acgtggcacc
     1621 gatcatccct gtgcttcgct cccatccgtt caacgacgat ccgctcggct agcgtgggag
     1681 acccgcaccc gcggacgcag ccgccagcgt ggcggcagca attggaggtc tggagtgccg
     1741 ggctgcaggg cggcctgctc tccctagccc gtgaccccac tgtcggtctc cggcgcctga
     1801 tcttcccggt cagttactgg cgtacccccg aatttgccta cgttcgccgc gaactccggc
     1861 tccccaacgg agcctcggtg ctggaccttg gtagcgccaa agagctggga acctggctgg
     1921 cccttcgacg gggctacgca gtgacctcgg cggacatcct tacagccgag gtctcgcgaa
     1981 ctgaacggta cgcgagtgca ctcgggatcc ggggttcgag cccaggccac ctgcgcgccg
     2041 aagtgcaaga cgggcgttcg ctgacgttcg cgaacgatgc ctttgacgcc gcgtactgcg
     2101 tatcgaccct gggcaacatt ccaggagatg gagattcaca aaccatccgt gagctattgc
     2161 gggtcgtcag agtgggcggt gtggtcgtcg tgaccgtgcc atttggcagc aaatactccg
     2221 agacctatgt caaccgtccg gttcacgagc gggactttga gggccagccg atcttctggg
     2281 aacgtcgcta cgaccgtcag tcgatggtgg atcggctgca gaaccttccc ggggctcgtc
     2341 tggtgagtct cgagttctgg ggggagcggg ttcggctgga gcgctggatc accgggcgag
     2401 gccctctgcg gttcgtcctc ctcccgctcg agcctctctt ggcgctactg gcgctccgcc
     2461 cgttggcgga cagcgaccgg gccacggtag cgttcttcac cctccagaag acctcgtaag
     2521 cgctcaagtc taacccgttt cccaagtcca cgtttaccgt tcaccgttta gtaaagcttc
     2581 tggcccttgg tccaaccgca cgcaaagggg cttccatagc tgcaaaaact ccatgcggcg
     2641 gttcaaatcc gcccggcgcc tctgccgggg caacagctta gggccgctcg aatgagcggc
     2701 cctcgtcatt ttggtaagct ttgggaagga aatctcgctg cggctgcgtg gcgcggtctc
     2761 gcggtccgct acaggggctt ggtgaaaccc gacgcgaacc ggccagtgag gccgagctga
     2821 attaagcgct tgtgctcagg cgtgcgccgc tgacgagcag agtccgaacc attacgagca
     2881 gggcatccgc ggtaaacggc ttttggagga acggggccaa tggggatggt gagccgcggt
     2941 ggaagacatc gtgttcggga tagcccgaga cgtacaggac tgggagtcct gggtgacgct
     3001 caccgatgag gcggcccaag gtacgcccat cggtttcagg catcactaca tcgactacag
     3061 ccaaatcaaa ccgagggtca gcccgctcga ggacgctcag cgcatcccgg gcattgctga
     3121 cctgaaccgc tacaaatccc gcctcagtga gcattcgaga aattacgaca cgcgccattt
     3181 cctcgtcgtc cactactaga atcgtcccgc cacccggcga acgcggtccg gctgccggta
     3241 ctaccatatt tcggcctcct gctggccgtt taggatctcc tgagaagcga cagacgaccg
     3301 gtcggaacgc caggatcttc gttttgagta gccgcgacag gcgacacatc ccctctcgac
     3361 gataatgagc ccactggcct catgacttca agggcccttc tccacttccg ccaacggcat
     3421 cttgcgcccg ccgctcgcat atcatgaaag agatggcacc gcgcccccgg gtctctcaag
     3481 gagaagccgg tgtgttcacc tgctgctcca gcaccgccga cgtactgccc gcgaagttgc
     3541 tatcgccgga gtactgagcg gtgatattgt gctggcccgc cgtgaagctc gagctcgtgg
     3601 tggcggatcc gttgccggac agagggacgg tggcgagcgt ggcatcgccg tcccggaatg
     3661 ccacggtccc cgttggagta ccctgtccgt tcgccacggc cactaccgcc gtaaacgtga
     3721 ccgactcgcc ctcgtcagcc gggttcttgc tggagctcac tgctgttgta gtagtggcac
     3781 gatttaccgt atacgagacg gtgggactct ggtcggtcac aaagtcagcc gtgctgggcg
     3841 tgaagacggc ttgaagactt ccggtgccgg cggcggtcgg ggtaaacgag cacttgccgg
     3901 cggtaacggg tgccgtacag ccgccggagt taccatacgt gacggtcacc gtacccaccg
     3961 gtgttccggc tccgggagga ttgacggtca cggtgaactg gaccgtgatc ggctggccaa
     4021 agaatgctgt cgccggggca ttggtgatca tgaccgtcgt ggccgcggca atcacctggt
     4081 gactcgttgc cttcgacgtg ctcgccgcga agtttgcatc gccgacgtag gtggccacca
     4141 ccgacttggt tcccgcggtg gtggaggtca acgcgcaact tccgctcggc gctgatgtcg
     4201 tgcaggtagc gccggtcccg tcgctcaccg agaccgtgcc gctcggtgtc ccactggccg
     4261 gcgcaaccgc cccgaccgtg aaggtcacgt cgtatggttg gcccacgacc gacgacgcgg
     4321 gggtgatacc ggtgatggct gtggccgact cgccggtcac gacgttgaac gccgcgctgg
     4381 ttgccctgac gagaccgcgg ccttgcgcag tcagcgtgta gccagtgccg cccgtcccga
     4441 ggctgagatc cgagaaggtg gccgtcccgt tggcggcctt gaccgaagtg acgccggcga
     4501 gcgacgccgt attcggtgcg ttgcccaggg taacagtgat tgtgtcgata gcggacgtga
     4561 ccgggttccc ggcgacgtct tgcacctgca ccttcacggg gggtgt
//
LOCUS       DASRJQ010000047         3931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2507252, whole genome shotgun sequence.
ACCESSION   DASRJQ010000047 DASRJQ010000000
VERSION     DASRJQ010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2507252"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VFB89_01125"
     CDS             <1..304
                     /locus_tag="VFB89_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25937.1"
                     /translation="ARLEVNLDTGRTHQIRVHMAHLGHPVVGDATYGGRVKKMLSLRE
                     PQRSLAIKLLGRLPRQALHALELQFLHPVTSKPLSFSSPWPNDVREAIELLSEGGS"
     gene            304..762
                     /locus_tag="VFB89_01130"
     CDS             304..762
                     /locus_tag="VFB89_01130"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZM25938.1"
                     /translation="MTMPVTQSKSPETIAVRIPSRLELLGILDRITETVCQRLQFDDD
                     TCCQVSMSVIEAGTNAIQHGHKRDASKPVDVEFRMMADQLEITVEDSGAGFDLGSVNG
                     DVTSPEHIFDSRGRGIFIMRSCMDRVDFSFTGSGTLVRLIKNRPTKAENR"
     gene            774..1190
                     /gene="ruvX"
                     /locus_tag="VFB89_01135"
     CDS             774..1190
                     /gene="ruvX"
                     /locus_tag="VFB89_01135"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HZM25939.1"
                     /translation="MRILAVDWGERRIGLAISDPTGVIATSLPTLEVGGRSEALAGVA
                     KVAAETEAECIVVGLPLLMSGARGSAAEAASAFAEALAARTQLTVETYDERLTSAMSQ
                     RRLHEAGVSTRRARGKVDQGAALALLQSFLQRRASR"
     gene            1240..2259
                     /gene="mltG"
                     /locus_tag="VFB89_01140"
     CDS             1240..2259
                     /gene="mltG"
                     /locus_tag="VFB89_01140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HZM25940.1"
                     /translation="MLLLLAAAAIVAYDLFLPAGLFPAPERRVILVQRGQTLRQIAGE
                     LQRVGLLRGTLTFHVLARLMHLDRSVKAGQYSFMLGTTVPGLLRSFARGMSGLNLVAI
                     PEGLTMTEVSLLLSNHLGVPVAEFDSLSRDRAFLDSLGVSAPSLEGYLAPDSYEFLPG
                     TLPEVALRTMVGRTRQILLRAAAGRDSLPLELSLYQLLTLASIVEAEAQMDDERPRIA
                     RVYINRLQMGMKLQADPTVGYAMGKGPRRRLYLSDLRTESPFNTYLHEGLPPGPICNP
                     GRASIEAALNPEPNGKDLYFVARGAGRHLFARTYAEHLENIRLVRRPTPPAEVRDSLT
                     AGVGG"
     gene            2298..3185
                     /gene="larE"
                     /locus_tag="VFB89_01145"
     CDS             2298..3185
                     /gene="larE"
                     /locus_tag="VFB89_01145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HZM25941.1"
                     /translation="MNSPLMHISEPPAPATEALWREKLAALREWFAPFSKVVVAYSGG
                     VDSSVVLRLAHEALGPGAIGVIGRSDSYAEREMRQALEQAGAFGARVEVVTTGELSDP
                     AFRSNPTDRCYHCKSELYRELAELARREGAQVVVDGTIADDLGDWRPGRRAAEEHEVR
                     SPLAELGFTKPEVRAVASVLGLGSSAKPASPCLASRIPYGTEITRENLHMVEAAEDLL
                     ISLGLTELRVRHHGDLARIEVPTSDFGRLLDRHTRESLIDGLKRLGYRHITLDLQGFR
                     SGSLNEGLSGLAGNTVKKT"
     gene            3278..3529
                     /locus_tag="VFB89_01150"
     CDS             3278..3529
                     /locus_tag="VFB89_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25942.1"
                     /translation="MKERKPVITRPRSLRKPVEVDLGFSWGLPNNVLLGLGLIALVMG
                     YLALSKGSTTLAPVLLVTGYCVLIPASLLLRGRTPGSGE"
     gene            3520..3595
                     /locus_tag="VFB89_01155"
     tRNA            3520..3595
                     /locus_tag="VFB89_01155"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3553..3555,aa:Val,seq:tac)
BASE COUNT          641 a         1315 c         1286 g          689 t
ORIGIN      
        1 agcgcgattg gaggtgaacc tggacaccgg ccggacccat caaattcgtg tacacatggc
       61 ccacctcggc catccggtgg tgggtgatgc gacatatggc ggccgtgtta aaaagatgtt
      121 gagccttcgt gagccacagc gtagcctcgc catcaagctc ttggggcggt tgccgaggca
      181 ggctctccac gccttggagt tgcagttctt gcacccagtt acctccaagc cgctgagttt
      241 ctcgtctccc tggcccaacg acgtccgtga agcaatcgag ctgctcagcg agggcggttc
      301 atgatgacca tgcccgtgac ccagtccaaa tctcccgaga ccatcgccgt gagaatcccc
      361 agccgcctcg aattgctggg tattctggac cgcatcaccg agaccgtttg ccagcgcctg
      421 cagttcgacg acgacacctg ctgccaggtg agcatgtcgg tgatcgaggc cggcaccaac
      481 gccatccagc atggccacaa gcgcgacgcc tccaagcccg tggacgtgga gttcaggatg
      541 atggccgacc agctcgagat cacggtcgag gacagcgggg cgggcttcga tctgggctcg
      601 gtcaacggcg atgtcacctc tcccgagcac atcttcgatt cgcgcggtcg cggcatcttc
      661 atcatgcgct cctgcatgga tcgggtggac ttcagcttca ccggctccgg cacgctggtt
      721 cgactgatca agaaccgtcc caccaaggcc gaaaaccgct gacgagaggt cgggtgcgga
      781 tcctcgccgt agactgggga gagcgccgca tcggcctcgc aatcagcgat cccaccggag
      841 tgatcgccac ctcgctgccc acgctcgagg tgggcgggcg ttccgaagcg ctcgccggcg
      901 tggcgaaggt ggcggccgaa acggaggcgg agtgcatcgt ggtgggtctc ccattgctga
      961 tgtcgggcgc gcgcggcagc gcggccgagg ccgcctcggc gttcgccgaa gccctggccg
     1021 cccgcacgca gctcacggtg gagacctacg acgagcggct cacctccgcg atgagccagc
     1081 gccgcctgca cgaggccggc gtgagcaccc gacgggcccg cgggaaggtg gaccagggcg
     1141 ccgcgctggc gctactccag tctttcctgc aacgtcgcgc gtcgcgctga gggccgcgtc
     1201 gtgacccgcc ggcgcacctt ccggcccagc cgttgggcgt tgctgctgct gctcgccgct
     1261 gcggcgatcg tggcctacga cctgttcctc cccgccggac tgttccccgc gcccgagcgg
     1321 cgcgtgatcc tggtgcagcg cggccagacg ctgcgccaga tcgcgggtga gctgcagcgc
     1381 gtcgggctac tgcgcggaac gctcactttc catgtgctcg cccgactcat gcacctcgat
     1441 cgcagcgtga aggccggcca gtacagcttc atgctcggca ccacggtgcc cgggctgctg
     1501 cgctcgttcg cgcgcggcat gagcggcctc aacctggtgg cgatccccga agggctcacc
     1561 atgaccgagg tcagccttct gctctccaat catctcggcg taccggtggc ggaattcgat
     1621 tcgctttctc gcgaccgggc gtttctcgac tcgctcgggg tgtcggcgcc cagcctggag
     1681 ggctacctgg cgcccgactc ctacgagttc ctgcccggca cgctccccga ggtggcgctg
     1741 cgcaccatgg tgggtcgcac gcgccagatt ctgctgcgcg ccgccgccgg acgcgactcg
     1801 ctgccgctcg agctgtcgct ctaccagctc ctcacactgg cctcgatcgt ggaggcggag
     1861 gcgcagatgg acgacgaacg cccgcgcatc gcgcgcgttt acatcaaccg cttgcagatg
     1921 ggcatgaagc tccaggccga tccgacggtg ggctacgcca tgggcaaggg accgcggcga
     1981 cgcctgtacc tcagcgatct gcgcaccgag tcgccgttca acacctacct gcatgaggga
     2041 ctcccgccgg gaccgatctg caacccgggc cgggccagca tcgaggcggc gctcaacccc
     2101 gagcccaacg gcaaggacct ctacttcgtt gcgcgcgggg ccggacgtca cctgtttgca
     2161 cgcacctacg ccgagcacct ggagaacatc cgcctggtgc gaaggcccac cccgccggcg
     2221 gaagtgcgcg attcgttgac cgcgggggtc ggcgggtagg cgggatcacg gcacgccccg
     2281 tgtgctactt ttgggccgtg aattctccgt tgatgcacat ctccgagcct ccagcgcccg
     2341 ccaccgaggc cctgtggcgc gagaagctgg ccgcgctgcg cgagtggttc gcgccctttt
     2401 ccaaggtggt ggtggcgtac tccggtggcg tggactcgag cgtggtgctg cggctcgccc
     2461 acgaggccct gggcccggga gccatcgggg tgatcggacg ctccgactcc tacgccgaac
     2521 gcgagatgcg gcaggccctg gagcaggccg gcgcctttgg cgcgcgggtg gaggtcgtga
     2581 ccaccgggga gctgtccgac cccgccttcc gcagcaaccc caccgaccgc tgctaccact
     2641 gtaagagcga gctatatcgc gagctggccg agctggccag gcgcgagggg gcgcaggtgg
     2701 tggtggatgg gaccatcgcc gacgacctcg gagactggcg cccgggccgg cgcgccgccg
     2761 aggaacacga ggtgcgctcg ccgctggcgg agctgggctt caccaagccc gaggtgcggg
     2821 cggtggcctc cgttctgggg ctggggagca gcgccaagcc ggcctccccc tgcctggctt
     2881 cccggattcc ctatggcacc gagatcaccc gcgagaacct tcatatggtc gaggccgccg
     2941 aggacctgct gatttcactt gggcttacgg agctgagggt gaggcaccac ggcgacctgg
     3001 cgcggatcga ggtcccgacc tccgactttg gccgcctgct cgaccgccac acccgcgaga
     3061 gtctgatcga cggtttgaag cgcctcgggt accggcatat cacactggat ctgcagggct
     3121 tccgttccgg tagcctcaac gaggggctgt ccggcctcgc cggaaatacg gtcaagaaaa
     3181 cgtaataatt tccggaacta cctcgtccca cccccgggta tgacaactac gctcagggtg
     3241 gtgaggcgag tccgccggcg tcctgaggga ggaagggatg aaggaacgca aacccgtcat
     3301 cacgcggcca cgcagtctca gaaagccggt cgaggtcgac ctcggattca gctggggcct
     3361 gccgaacaac gtgctcctgg gactgggtct cattgccctc gtgatgggtt atcttgcgct
     3421 ctccaagggt tcaacgacgc tcgctccggt gctgctggtg accggttact gcgtcctcat
     3481 cccagcttct cttctgttac gcggtcggac cccgggttcg ggcgaatagc tcagctggca
     3541 gagcacttgc cttacaagca aggggtcaca ggttcgagcc ctgtttcgcc caccatcgaa
     3601 cgagtgaacc gctgtacggt ttatttacag gcggccggcc atccgggcgg tcgccgtcga
     3661 tgaggggcag aacgtcagca attcgatctt gacacgccgt tgaccccgac ctagactttg
     3721 ttcgtctcgg aaggccttgt aatcgcggca attactgttt cgctgacggt taggactcgc
     3781 tgcgtcaacg agttcaaaag gaggtggctc gcgccagacc tgattgcttg caactgagcc
     3841 ttgcaacgat tctaggcaac aggtgagttc actacggagg taagacatga ctggtttgat
     3901 tcgcagggcc acgcttctta ccgcggctgg c
//
LOCUS       DASRJQ010000048         2336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_538405, whole genome shotgun sequence.
ACCESSION   DASRJQ010000048 DASRJQ010000000
VERSION     DASRJQ010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538405"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1937)
                     /locus_tag="VFB89_01160"
     CDS             complement(123..1937)
                     /locus_tag="VFB89_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25943.1"
                     /translation="MRPHRLRWLSLVLAVLPVISASAHETLRISTPMPPPVWALLERQ
                     LLKSNEDACLQTLELLFDERGYMKCYERWGADDGPDDAIETASDWPLLHALGASDQVL
                     ASYRKVWEGHLQQYTHARTTQVEFAKNGMYYKEFPVCLDWQHIGEGLSAFNLQGLSDP
                     YDERFRQRVRRFAGFYLNEDPEAPNYDPKHKIIRSTFNGSRGPLLRQATALDWVGDPL
                     PDRHFRLLHGEANYQEMLDHYRDYTDVVGDHPQNLCATVLAANAFMLEHEAKYRDWVV
                     EYVNAWRDRTLANGGNIPSNIGLDGRVGSAYGGAWYSGVYGWDFNPYDPPTKKKQSRS
                     FIYRGPWMGFANALLLTGDPSYLDVLRGQVANVYAAKKIIDGQTMIPRMHGAQGWYGY
                     TTDLLAHRLLKIYLWSFRDDDRARVPKDEWLEFLEGRNPGFPEQAMGRDFALVRQNAE
                     RMRADTLTPDTRLSDNALPYIAVNVGSLVQLMLGALPTWKDGDLLHAQIRYFDPERRR
                     SGLPESVGALIDGLSADGVSLTLVNLDQVKPRSVVVQAGAYAEHDFTAVTVGEKTTEV
                     GRSFVEIRLAPGAGSKITAKMKRYANRPTLSMPWDRAR"
     gene            complement(2062..>2336)
                     /locus_tag="VFB89_01165"
     CDS             complement(2062..>2336)
                     /locus_tag="VFB89_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25944.1"
                     /translation="ARQLASNLRTFPSRLSGVRVEGLKNVDMSLLKNTRITEGVTIQF
                     RAEALDVLNHHNYTEQPNTTPTSTAFGTSTFGRGNREIQLGLKLIF"
BASE COUNT          429 a          723 c          719 g          465 t
ORIGIN      
        1 acgaacctgc ccgcttcacc gttccgtacg gacgactggc cgctcgacac ggaacccgcc
       61 acgcgctgat tcgacgccag tcgagcgccg gtaggattgt gaccagcacc cggtagctga
      121 cgctaacggg cccggtccca gggcattgag agcgtggggc gattggcata acgtttcatc
      181 ttcgcggtta tcttgctacc cgcgcccggc gccagtcgga tctcgacgaa agaacggccg
      241 acttcggttg ttttttcgcc gaccgttact gcggtgaagt cgtgctcggc gtaagccccg
      301 gcctgcacta ccacgctacg cggcttgacc tgatcgagat tgacgagcgt gagagagact
      361 ccgtccgccg atagcccgtc gatgagagcg cctaccgatt cggggagccc gctccgtcgg
      421 cgctccggat cgaagtaacg gatctgcgca tggagcaggt cgccgtcctt ccaggtcgga
      481 agggcgccca gcatgagttg gaccagcgat ccgacgttga ccgcgatata gggcagggcg
      541 ttatcggaaa gtcgagtgtc gggagtgagc gtgtctgcgc gcatgcgctc ggcattttgc
      601 ctcaccaacg cgaagtcgcg gcccatcgcc tgttcgggaa agccgggatt ccggccctcc
      661 agaaactcca gccactcgtc tttcggcacc cgcgcgcgat cgtcgtcacg gaacgaccag
      721 aggtagatct tgagcagccg atgcgcgagc aggtcggtgg tatacccgta ccagccctgc
      781 gcgccatgca tacgtggaat catggtctgt ccgtcgatga ttttcttggc cgcatacaca
      841 ttggcgacct ggccgcggag aacatcgagg taactcggat ctccggtcag cagcagggcg
      901 ttggcgaacc ccatccacgg gccgcgatag atgaagcttc ggctctgctt cttcttcgtc
      961 ggcggatcgt agggattgaa gtcccagcca tagacgccgc tgtaccaggc gccgccgtag
     1021 gcacttccta cccggccatc gagaccgata ttgctcggta tattgccgcc attggccagc
     1081 gtgcgatccc gccacgcgtt tacatattcc acaacccagt cccgatactt cgcttcgtgc
     1141 tcgagcatga acgcatttgc ggcgagcacg gtggcgcaca ggttctgggg atgatcgccg
     1201 acgacatccg tgtagtcgcg gtagtgatcc agcatctcct ggtagttggc ctcgccgtgc
     1261 agcaggcgga aatgccggtc tggaagcgga tcgcctaccc agtcgagagc cgtcgcctgc
     1321 cggagcagag gtccccggct gccgttgaaa gtgctgcgga tgatcttgtg cttcggatcg
     1381 tagttcggcg cttcaggatc ctcgttgagg tagaagccgg cgaagcggcg cacccgctgg
     1441 cgaaaacgct cgtcgtaggg atcgctcagt ccttgcaggt tgaaggccga aagcccctcc
     1501 ccgatatgct gccagtcgag gcagacaggg aattccttgt agtacatccc gttttttgcg
     1561 aactcgacct gagtggtccg ggcgtgcgtg tattgctgca aatggccctc ccagaccttc
     1621 cggtaacttg cgaggacctg atcggatgcg cccagggcgt gcaggagcgg ccaatcgctg
     1681 gccgtctcga tggcatcgtc cggcccgtcg tccgcacccc agcgctcgta gcacttcatg
     1741 tagccgcgct cgtcgaagag cagctcgagc gtttgaaggc acgcgtcctc gttggatttc
     1801 agcagctggc gctcgagcag cgcccagacc ggaggaggca tgggcgtgct tatccgcagc
     1861 gtttcgtgag cggatgcact gatgacgggc aggacagcca gaaccagcga caaccatcgc
     1921 aatcgatggg gcctcatgat ttctccgcta gattcccggc ggcatccccc gccagggcaa
     1981 cgccgccatc atatccgact ttcgcctacc ttcccgacgc ctctcacaat gcgtttgagc
     2041 gtggcaggga gtatgtctga ctcaaaatat tagtttcaat cccagctgga tctctcggtt
     2101 accccggccg aacgtgctgg tgccgaatgc cgtcgaggtc ggggtcgtgt tcggctgctc
     2161 ggtgtagtta tggtgattca gcacgtcaag cgcctcagcg cggaactgga tggttacacc
     2221 ctccgtgatc cgggtgttct tcagcagaga catatcaaca ttcttcagcc cctcgacccg
     2281 gacacccgag aggcgggagg gaaacgtgcg caagttggac gccagctgac gagccg
//
LOCUS       DASRJQ010000049         2399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2216338, whole genome shotgun sequence.
ACCESSION   DASRJQ010000049 DASRJQ010000000
VERSION     DASRJQ010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2216338"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="VFB89_01170"
     CDS             <1..701
                     /locus_tag="VFB89_01170"
                     /inference="COORDINATES: protein motif:HMM:NF017509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25945.1"
                     /translation="YTLVIGERQAIPELEERIMTLLPGETMETEVRFPDDHPDASRRG
                     QTRKVRVHLLELKRQELPALDDAFAREVGEFENLGALRAAVRQDLERDATREADANVR
                     QALLAQLIEANRIPAPDSLVHRVLHGFAHMYKIPDDQMAGFEAQFRPIAVQQVQRDLV
                     LEAAAEAHGLKATEADLDERIATMAAARNLPTGELYASLQKSNRLAELERAITEEKVF
                     NHLLSQSTVEEAST"
     gene            704..1345
                     /gene="clpP"
                     /locus_tag="VFB89_01175"
     CDS             704..1345
                     /gene="clpP"
                     /locus_tag="VFB89_01175"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HZM25946.1"
                     /translation="MATLYPPYIIERSSRGERTYDIFSRLLMDRIVFLGAPINDDVAN
                     IIIAQLLFLEADNPDKDIYLYINSPGGVVSAGMAIYDTMQHLRAPVNTICMGMAASMG
                     SFLLCAGAPGKRSALPHARIMIHQPSGGAQGTAADIEIQAREILYLRSKMNTLYAKHT
                     GQPLETIERDMDRDRFMSSDEAKAYGLIDSVFIPRKEFVVPSKNGAKETLTPS"
     gene            1387..>2399
                     /gene="clpX"
                     /locus_tag="VFB89_01180"
     CDS             1387..>2399
                     /gene="clpX"
                     /locus_tag="VFB89_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HZM25947.1"
                     /translation="MSNDKHLRCSFCGKSKDSVRKFISGPSVYICNECITLCNEILAE
                     DEEREVADSITQVPTPTEIKDVLDQYVIGQERAKRTLAVAVYNHYKRINASNGREAEV
                     ELDKSNILLLGPTGVGKTLLAQTLARILDVPFTIADATTLTEAGYVGEDVENILVRLL
                     QAGDFNVAECERGIVYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVASVPP
                     QGGRKHPQQEYIQINTKDILFICGGAFDGLEKIIQARTGRQQIGFSSEASGRKAEPDQ
                     KNPYADVEPDDLLRFGLIPELVGRLPVVVSLEALDEDALMRILIEPKNALTKQYKKLF
                     ELE"
BASE COUNT          497 a          771 c          738 g          393 t
ORIGIN      
        1 cctacacgct agtgatcgga gagcgccagg ccatccccga gctggaagag cggatcatga
       61 cgctgctccc tggcgaaacc atggaaaccg aggttcgatt cccggacgat caccctgacg
      121 cgagccggcg cgggcagacc cgaaaggtcc gggttcatct gctcgagcta aagcgacagg
      181 agcttcccgc gctggacgac gcgttcgccc gcgaggtcgg cgagttcgag aatctgggcg
      241 cactgcgggc tgctgtccgc caggacctcg agcgcgacgc cacccgggag gctgatgcca
      301 acgtgcgaca ggcgctgctg gctcaactca tcgaggcgaa ccggatccct gccccggact
      361 ccctggtgca ccgggtactg cacggcttcg cccatatgta caagatcccg gacgatcaga
      421 tggccggctt cgaagcgcaa ttccggccca tcgcagtgca acaggtgcag cgcgacctgg
      481 tgctggaagc tgcagcggaa gcgcacgggc tcaaggcgac ggaagctgac ctggacgagc
      541 gaattgccac catggcggcg gcacggaacc tgcccactgg tgagctttac gcctcgttac
      601 agaagtcgaa tcggctcgcg gagttggagc gggccatcac cgaggagaag gtgttcaacc
      661 atctcctctc gcagtccacg gtcgaagagg cgagcacctg acgatggcca cactatatcc
      721 gccgtacatc atcgagcgtt cgagccgcgg ggagcggacc tacgacatct tctcccggct
      781 cctcatggac cgcatcgtgt tccttggtgc gccgatcaac gatgacgtcg ccaatatcat
      841 cattgcccag ctcctctttc tcgaggctga caatccggac aaggacattt acctgtacat
      901 caactcgccg ggcggggtgg tgtcggccgg aatggcgatc tatgacacga tgcagcacct
      961 tcgcgccccg gtgaacacga tctgcatggg gatggccgcg tccatgggct ccttccttct
     1021 ctgtgccggt gcccccggca agcggagcgc cctcccccac gctcggatca tgatccacca
     1081 gccctcgggt ggcgcccagg gcaccgcggc ggacatcgaa attcaggctc gcgagatcct
     1141 gtacctgcgc tccaagatga acacgctgta cgccaagcac accggccagc cgctcgagac
     1201 gatcgagcgg gacatggatc gggaccggtt catgagctcc gacgaagcaa aagcctacgg
     1261 cctgatcgac tcggtgttca ttccgcgcaa ggaattcgtg gtgccgagca agaacggcgc
     1321 gaaagagacc ttgacgccct cctgagcggg gggcagcttt ccgggaccgc gcaaccagtc
     1381 accgccatgt ccaacgacaa gcaccttcgt tgctcctttt gcgggaagtc caaagactcg
     1441 gtccggaagt tcatctccgg gccgtcggtc tacatctgca acgagtgcat cacgctctgc
     1501 aacgaaattc tggcggagga cgaagagcgg gaggttgccg acagcattac ccaggtcccc
     1561 acgccaaccg aaatcaagga tgtgctggac cagtatgtga tcgggcagga gcgggccaag
     1621 cggaccctgg cggtggcggt ctacaatcac tacaagcgca tcaacgcgtc caacggacgc
     1681 gaggcagagg tcgagctgga caagtcgaat attctgctcc tcggcccgac cggcgtcggc
     1741 aagacgctgc tcgctcagac cctcgcccga attctcgacg ttcccttcac tattgccgat
     1801 gctaccacgc tcaccgaagc aggctacgtc ggtgaggatg tcgagaacat tctggtacgc
     1861 ctgctccagg ccggggactt caatgtcgcc gagtgtgagc gcggcatagt ttacatcgac
     1921 gagatcgaca agatcgccag gaagtcggaa aacccgagca tcacccggga cgtctcgggc
     1981 gagggcgtgc agcaggccct gctcaagatc ctcgagggca ccgtcgcctc ggtgccgccg
     2041 cagggcggtc gaaagcaccc gcagcaggag tacatccaga tcaacaccaa ggacatcctg
     2101 ttcatctgcg gcggtgcgtt cgacggactg gagaagatca ttcaggcccg caccgggcgg
     2161 cagcagatcg gtttcagcag cgaagccagc ggccgcaagg ccgaaccgga ccagaagaac
     2221 ccctacgccg acgtcgagcc cgacgatctg cttcggttcg ggctcatccc cgagttggtc
     2281 ggccggcttc ccgtggtggt gtcgctcgaa gcgctggacg aggacgcgct catgcggatc
     2341 ctcatcgagc ccaagaacgc gctcaccaag cagtacaaga agctgttcga gctggagga
//
LOCUS       DASRJQ010000050         4246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1646831, whole genome shotgun sequence.
ACCESSION   DASRJQ010000050 DASRJQ010000000
VERSION     DASRJQ010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1646831"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VFB89_01185"
     CDS             complement(<1..551)
                     /locus_tag="VFB89_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZM25948.1"
                     /translation="MIELQGLSKTYRSLFGTRVVPALDDLTLTLARGEVVGVAGPNGA
                     GKSTLISLLLGYLRPTQGQVQVDRMPPRRWVERHGVGYQAELVAIPGRWSVEGALQRY
                     TVLSGVPRAQLNQRVDALLSDFGLAEQRRKRVRELSKGNLQRLGLAQALAGDSDLVVL
                     DEPTHGLDPLWTQRFRDVVRDLRR"
     gene            complement(548..1681)
                     /locus_tag="VFB89_01190"
     CDS             complement(548..1681)
                     /locus_tag="VFB89_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM25949.1"
                     /translation="MSDAFRSSDDFDEQAHQLYNEGRYDEAVALLREGLSLYPNAVEL
                     HIGLGYAQLAREEFAWARRSFERALTFDPDHEDGLAGLGEVLLKLGERGGALRAFERI
                     VLLGFQEDHELMLQVGRALFREALVGPAQRFFEFAVTAHQESPDAAACLGYACHRLGS
                     DSGALFWLRRSLELEPDFVEARIYLANMLYDRGETEAALHHLEKTRPEEHYDELGIWR
                     HIELKKTVYHLRDEDPELQPWLQRLGEVAGDPEPVDLLLAEVEALQPDGSIRDPHQLE
                     LFGTLLTELHAMQRRPGQPVAHPTDTHAVGTLGGLTLKGTWDEILLQMKAADHEWADA
                     SLTEFMVGLARRGRTETGVVIPVTDAEAFIRGSAEAGVLRIIQ"
     gene            1881..2543
                     /locus_tag="VFB89_01195"
     CDS             1881..2543
                     /locus_tag="VFB89_01195"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZM25950.1"
                     /translation="MSQPADTTNPPAPRWQRRPQERPEEILDAAFDVFGEQGFARTRL
                     EEVAQRAGVSKGTLYLYFDSKETLFREMVRAKAVAALREAEAFVEGFQGTNRDLLITF
                     LRRMYRVLRQQNLTRISRLVQAELGSFPELAHFYTQEVILPARRLMETIIVRGVASGE
                     FRETPLGFASRAVPMLLIHAAQTQCFFQRFDPEALSDEETLDGVIDFCLSGVLARPEP
                     QR"
     gene            2548..3873
                     /locus_tag="VFB89_01200"
     CDS             2548..3873
                     /locus_tag="VFB89_01200"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZM25951.1"
                     /translation="MVRSTVVLLAFLLLAAPAGAQQPAPPATPQGPLTLLDAIRIGRQ
                     RAVSAVLAQINARIAGERVGERRADLLPTINGAAAASRQTLNLQEFGLTFPGFPPKVP
                     DFTLWRFRLDGSQTLFNASVFRRLKSAQDSAAAAGLDAQAVGEIAGATAGLAYLQVQS
                     AQETVRAREADSVVAAALLDQARQLVSAGVSPTIDATRSEVSFQAVRTQLELARNARD
                     RTRLDLLRTLDYPPGAKLQVADSLGQQAVELPLDPDTAGQFARQHRAELAAERKRTEA
                     LRRSLSAIKAENLPSLNATGYYQQTGPALGDLIGTYQVLVGLSVPILDGLKRQVRARE
                     AQARVEAQQVREGDLGNQVETEARQAVLDIASARQQVAIAVDQLRLAEQELAQADERF
                     RAGVAGSVETTNAQSSVIAARDAVIQARVNYGSARVSAYRALGIIDQLQ"
     gene            3894..>4246
                     /locus_tag="VFB89_01205"
     CDS             3894..>4246
                     /locus_tag="VFB89_01205"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HZM25952.1"
                     /translation="MATDIAMDLPATDTRPEAGTRRKWLPLVIVGLVVAATATWGIRR
                     YVWGLHHESTDNAQLDGHITVIAPRIQAFVSRVLVDDNQHVKAGDTLVVLDDRDLKVR
                     LQQAQADLADAASSAG"
BASE COUNT          754 a         1377 c         1385 g          730 t
ORIGIN      
        1 cgtcggagat cccgcaccac atcgcggaag cgctgggtcc acagggggtc gaggccgtgg
       61 gtcggctcgt ccagtaccac cagatcactg tcgccggcaa gagcctgcgc cagaccgagc
      121 cgttggagat ttcccttgga cagctcgcgc actcgcttcc gccgctgctc cgcaaggccg
      181 aagtcgctca gcagagcgtc gacccgctga ttcagttgag ccctgggaac gccggaaagc
      241 acagtgtatc gctgcagcgc tccttcgacg ctccagcgcc cgggaatggc gaccagctcg
      301 gcctgatagc cgacgccgtg acgctccacc cagcgacgcg gcggcattcg gtcgacctgg
      361 acctgcccct gcgtcggacg gaggtatccg agcaacagcg agatgagcgt gctcttgccg
      421 gcaccgttag gcccggcaac gccgacgacc tcaccccgcg cgagtgtgag ggtgaggtcg
      481 tcgagcgccg gcaccacccg cgtgccgaag agcgatcggt acgttttgga aaggccctgc
      541 agctcgatca ctggataatg cggagcaccc ctgcctcggc gctgccacgg atgaacgcct
      601 cggcgtccgt aaccggaata acgacgccgg tctccgtgcg accccgccga gcgaggccga
      661 ccatgaactc ggtgagcgag gcatcggccc actcgtggtc ggccgccttc atctgcaaca
      721 ggatctcgtc ccaggtcccc ttaagcgtga gtccacccaa cgtcccgacc gcatgtgtat
      781 cggtcggatg cgcgaccggc tggcccgggc ggcgctgcat cgcgtgcagc tcggtgagca
      841 gcgttccgaa cagttccagc tggtgcgggt cacgaatgct gccgtcgggc tgcagagctt
      901 cgacctcggc cagcaggagg tcaactggct ccggatcgcc ggcgacttcg ccaagccgct
      961 gcaaccaggg ctgcaactcg ggatcttcat cgcggaggtg gtagaccgtc ttcttgagct
     1021 cgatgtggcg ccagatgccc agctcatcgt agtgctcttc ggggcgagtc ttctcgagat
     1081 ggtgcagcgc cgcctcggtc tcgccgcggt cgtacagcat gttggcgaga tagatccgcg
     1141 cttccacaaa gtcgggctcg agctcgaggg atctgcgtag ccaaaacagc gccccgctat
     1201 cgctgcccaa ccgatggcaa gcatatccca ggcacgccgc agcgtccggc gactcctggt
     1261 gcgccgtgac ggcaaactcg aagaagcgct gcgccggacc gaccagagct tcccggaaca
     1321 aggctctgcc cacctgcaac atcagctcgt ggtcttcctg gaatcccaga agcacgatcc
     1381 gctcgaacgc acgcagtgct ccgccacgct cgcccagctt gagcagcact tcgcccagac
     1441 cggcaaggcc gtcctcatgg tccgggtcga acgtgagagc acgctcgaag ctcctccggg
     1501 cccaggcgaa ctcttcccgg gcaagctggg cataacccag tccgatgtgg agttctaccg
     1561 cattgggata gagggaaagt ccctcgcgga ggagagcgac cgcctcgtcg tagcggcctt
     1621 cattgtagag ctgatgggct tgttcgtcga aatcgtcgga gctgcggaat gcgtccgaca
     1681 tcgagagggc ctcccgatga gctttgggtc aaaagtagga cggtcgatgg ggtggaagca
     1741 agtcaagcgg tcatcggcgt ccgtcctgga acggacgcct aattagcggg cccgtcgccg
     1801 cttagccggc cgactgtagg gggttgacgg ttgaggtgtc aatgttagct tactgaccag
     1861 tcagttacta agggtcacga atgagtcagc ccgccgatac caccaacccg ccggcccctc
     1921 ggtggcaacg ccgcccgcag gagcgccctg aggagatcct cgacgccgct tttgatgttt
     1981 tcggagaaca aggattcgcc cgcaccaggt tggaggaagt cgcacagcgg gcgggcgtca
     2041 gcaaggggac tctctacctc tacttcgatt cgaaagagac gctttttcgc gaaatggttc
     2101 gggccaaggc agtcgcggcg ttacgcgagg ctgaagcttt cgtcgaggga ttccaaggga
     2161 cgaatcgcga tctgttgatc acgtttctgc gccgcatgta ccgcgtgttg cggcagcaga
     2221 acctgacccg gatcagtcgg ctggtgcagg cggagttggg aagctttccc gagctcgccc
     2281 acttctacac acaggaggtc attcttccgg ctcgccgcct gatggagacg atcatcgttc
     2341 gcggtgtggc ctccggtgag tttcgagaga cgccgctcgg ctttgccagt cgtgccgtgc
     2401 cgatgctcct gatccacgca gcacagacgc agtgcttctt ccagcggttc gatcccgaag
     2461 cgctgtcgga tgaggaaacg ctcgatggcg tcatcgactt ttgcctgagc ggcgtgcttg
     2521 cgcgtccaga gcctcaacgg tagcccaatg gtgcgctcaa ccgttgtcct tctcgcattc
     2581 ctgttgttgg ccgctcccgc cggagcgcag cagccggctc cgcctgcaac gccgcaagga
     2641 ccgctgacgc tgctcgatgc tattcgtatc ggccggcagc gggcagtctc cgccgtgctg
     2701 gcgcaaatca atgctcgcat cgcaggtgag cgggtcggcg agcggcgggc ggaccttctg
     2761 cccacgatca acggggccgc ggcggcttcc cgccagacgc tgaatctcca ggaattcggc
     2821 ctcacctttc ccggtttccc accgaaggtg ccggacttca cgctatggcg attccgtctg
     2881 gatgggagtc agacgctgtt caacgccagc gtatttcgcc ggctcaagtc ggctcaggac
     2941 agcgccgcgg ccgccggact cgatgcccag gcggtaggtg agatcgccgg agctacggca
     3001 ggtctggcgt acctccaagt ccagagcgcg caggaaaccg tacgagcccg tgaggccgac
     3061 agtgtggtgg ctgccgccct gctcgaccag gctcgccagc tggtcagtgc cggcgtcagc
     3121 cccaccatcg atgccacgcg cagcgaggtg agtttccagg cggtgcggac ccaattggag
     3181 ctggcgcgca acgctcggga tcggacccgg ttggatctgc tccgcaccct cgactatccc
     3241 ccgggggcga agcttcaagt tgccgactcg ctgggccagc aggccgtcga gcttccgctg
     3301 gaccccgaca ccgcgggcca gtttgcccgt cagcaccggg ccgagcttgc ggcggagcgg
     3361 aagcggacgg aagcgctccg tcgctccctg tccgcaatca aggccgagaa cctccccagt
     3421 ctgaatgcga ccggctacta ccagcagacc ggaccggcac tgggagatct gatcggcacg
     3481 tatcaggtgt tagtaggcct gagcgtcccc attctcgatg gcctcaaacg acaggtccgc
     3541 gctcgggagg cgcaggcgcg ggtggaagct cagcaggtcc gggaaggcga tctgggcaat
     3601 caggtggaga cggaagccag gcaggcggtg ctcgacattg cctcggctcg gcaacaggtg
     3661 gcgatcgcgg tggatcagct gcgactcgcc gagcaggagc tggcgcaggc ggacgagcgg
     3721 ttccgcgcgg gcgtggcggg atcggtcgag acgacgaacg cacagagctc tgtgattgcc
     3781 gcccgcgacg ccgttataca ggcgcgggtg aattatggca gtgcccgggt gagcgcgtac
     3841 cgcgccctcg ggatcatcga ccagttgcag tagaaccggg aacgagacgc atcatggcaa
     3901 ccgatatcgc aatggatctt cctgccaccg acactcggcc cgaggcgggg acgcggagga
     3961 agtggcttcc cctcgtgatc gtcggccttg tcgtcgccgc tacggctacg tggggcattc
     4021 gtcgatacgt gtgggggctg catcacgaat ccaccgacaa cgcgcagctc gacggccaca
     4081 tcaccgtcat cgcgccgcgg attcaggcgt tcgtctcccg ggtgttggtc gacgacaacc
     4141 agcacgtgaa ggcaggagac accctcgtcg tcctggatga ccgagacctc aaggtccggc
     4201 tgcagcaggc gcaggccgat ctcgccgatg cggcctcgag tgccgg
//
LOCUS       DASRJQ010000051         3042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1938558, whole genome shotgun sequence.
ACCESSION   DASRJQ010000051 DASRJQ010000000
VERSION     DASRJQ010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1938558"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VFB89_01210"
     CDS             <1..400
                     /locus_tag="VFB89_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25953.1"
                     /translation="LLDQRRAVGPALAGAWELVDCVLDIAPAARAQTIKVEASLTPSA
                     DLDVRIGRSPGDDAVTEDGVLTTPPEVPPPDPEGQERWARNWAEVRVMEKRRRNFAEA
                     DRIRTLLRKHGWEVQDAKDGSIQLRKLGGG"
     gene            455..527
                     /locus_tag="VFB89_01215"
     tRNA            455..527
                     /locus_tag="VFB89_01215"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:488..490,aa:Thr,seq:tgt)
     gene            614..697
                     /locus_tag="VFB89_01220"
     tRNA            614..697
                     /locus_tag="VFB89_01220"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:648..650,aa:Tyr,seq:gta)
     gene            741..813
                     /locus_tag="VFB89_01225"
     tRNA            741..813
                     /locus_tag="VFB89_01225"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Gly,seq:tcc)
     gene            831..903
                     /locus_tag="VFB89_01230"
     tRNA            831..903
                     /locus_tag="VFB89_01230"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:864..866,aa:Thr,seq:ggt)
     gene            1047..1199
                     /gene="rpmG"
                     /locus_tag="VFB89_01235"
     CDS             1047..1199
                     /gene="rpmG"
                     /locus_tag="VFB89_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HZM25954.1"
                     /translation="MPRDKIILECTECRSRNFFQDKNRRKHPERVEYRKYCPRCDKHR
                     VHKESK"
     gene            1196..1441
                     /gene="secE"
                     /locus_tag="VFB89_01240"
     CDS             1196..1441
                     /gene="secE"
                     /locus_tag="VFB89_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HZM25955.1"
                     /translation="MNSDVQAATPPGKISGAVAQAGGFFKAVRAELDKVTWPTRPELV
                     KATRMIVILSIALGIAIGLLDWVLQKILVDGVAALAR"
     gene            1444..1986
                     /gene="nusG"
                     /locus_tag="VFB89_01245"
     CDS             1444..1986
                     /gene="nusG"
                     /locus_tag="VFB89_01245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HZM25956.1"
                     /translation="MEYRWYAIQTTAGHENKVRNLLGRRITDDPRPEDQKLIRQALVP
                     VQEVIEIKNGKKVNVERKLYPGYVLVEMQMSQEALHVVNSIQGVIKFVGSGKLPQPLR
                     QDEVNRLLGIAEAVAEAEPKEEIPFMVGQVVEITEGPFSDFSGTVEEVLGDKGKVKVS
                     VSLFGRPTAVELDYTQLRGH"
     gene            2066..2491
                     /gene="rplK"
                     /locus_tag="VFB89_01250"
     CDS             2066..2491
                     /gene="rplK"
                     /locus_tag="VFB89_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HZM25957.1"
                     /translation="MAKKIQAIVKLQCPAGAASPAPPVGTALGPHGVNIMEFVKQFNA
                     RTQSQAGMVIPVVVTIFADRTFNFITKTPPAPNLLLKEAGIEKGSAQPNRTKVGKVTR
                     AQLKKIAEIKMQDLNASDLDSAMRMIAGTARSMGLEVAD"
     gene            2491..>3042
                     /gene="rplA"
                     /locus_tag="VFB89_01255"
     CDS             2491..>3042
                     /gene="rplA"
                     /locus_tag="VFB89_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HZM25958.1"
                     /translation="MPAVGKKFRAAQGKVDRTRHYPVLEAVGLVKQSAFAKFDETVDV
                     AVNLGVDPRHADQVVRGTVVLPHGTGKSVRVLVIAQGDKAKEAEAAGADFVGIEYVQK
                     IKDGWLDCDVIVATPDVMGQLGQLGRVLGPRGLMPNPKAGTVTLDVTKAVREIKAGKI
                     EFRVDKTGNVHAPVGKVSFNAEQL"
BASE COUNT          617 a          867 c         1050 g          508 t
ORIGIN      
        1 tctcttggac cagaggcggg ctgtaggtcc tgcgctggca ggggcttggg agctggtgga
       61 ctgcgtgctc gacatcgcgc cggcggcccg ggctcagacc atcaaggtcg aagcaagcct
      121 gacgccgtcc gcggatttgg acgtccggat cggccggtcg cccggcgacg acgccgtaac
      181 cgaggacggt gtgttgacga cgccgccgga agtaccgcca cctgaccccg aaggccagga
      241 gcggtgggct cgcaattggg ctgaagtcag agtaatggag aagaggcgcc ggaattttgc
      301 cgaggccgac cggatcagga ccctccttcg gaagcacgga tgggaggtcc aggacgccaa
      361 ggacggatcc atccaactcc ggaagctcgg gggcggttga cgcacgctaa cttggcgtct
      421 atcatatggg tctggctctt ttcggcgtgc tttcgctgac gtagctcaac tggcagagca
      481 ccggttttgt aaaccggcgg ttaggggttc gattcccctc gtcagctcgg tgatgccgac
      541 acccgcagcg caacatgacc gaaccccgcc gacgcggcgg ggttctgtct cgctggggtg
      601 agaggggcga agcggtgggg tggccgagtg gctaatggca gcagactgta aatctgccgg
      661 gctttgccct acgtaggttc gaatcctacc cccaccatgg gaggcggagg gcgaggggca
      721 ggagaagacg gcgcaggctc gcgggcgtag ctcagttggt agagcgtcag ccttccaagc
      781 tgaatgtcgc gggttcgagc cccgtcgccc gcttatcggt tgagcggttt gctcgcgtag
      841 ctcagtcggt agagcgcatc cttggtaagg atgaggtcac cggttcaatc ccggtcgcga
      901 gcttggtgga agctatcagc gatcagctat ccgctggggc taaggcgggc gcgagacgca
      961 gaaacaagct gatagctgat gactgatggg tgatgactga tagctgatga ctgatagctg
     1021 atagccgcga gcgacagcga gcaaacatgc cccgcgacaa gatcattctc gaatgcaccg
     1081 agtgccggag ccgcaacttc ttccaggaca agaatcggcg gaagcacccg gagcgcgtgg
     1141 agtatcggaa gtactgcccc cggtgcgata agcatcgggt gcacaaggag tcgaagtgaa
     1201 ctcagacgtt caggcagcga caccgcccgg gaagatctcc ggtgcggtgg ctcaggccgg
     1261 cgggttcttc aaggcggtgc gggccgaact cgataaggtg acctggccaa cccggccgga
     1321 actggtcaag gccacccgga tgatcgtgat cctctcgatc gcgctcggca tcgccattgg
     1381 cctgctcgac tgggtgctcc agaagatcct ggtggacggg gtcgcggcac tggctcggtg
     1441 acgatggaat accgctggta cgcgatccag accacggctg gacacgaaaa caaagtgcgg
     1501 aacctcctgg gtaggcgaat caccgacgat ccgcggcccg aggaccagaa gctgattcgc
     1561 caggcgttgg tgccggttca ggaagtgatc gagatcaaga acggcaaaaa ggtcaacgtc
     1621 gagcgcaagc tctaccccgg gtacgtgctg gtcgagatgc agatgagcca ggaggccctg
     1681 catgtcgtca acagcattca aggcgtcatc aagttcgtgg gctcgggcaa gctgccccag
     1741 ccgctccgcc aggacgaggt caaccgcctg ctcggcatcg ccgaggcggt cgccgaggca
     1801 gagccgaaag aggagatccc cttcatggtg ggccaggtgg tcgagatcac cgaaggcccg
     1861 ttctccgatt tcagcggcac ggtggaggaa gtcctgggcg acaagggcaa ggtgaaggtg
     1921 agcgtgagcc tgttcgggcg gcccacggcc gtcgagctgg actacacgca gctgcgcggg
     1981 cactgaggcc cgctcccgcg accgcgcggg tcatcgcggc agccgtcacg cgagttggat
     2041 gcggactcgg cgttagagga gatgtatggc gaagaagatc caggcaattg tgaagctcca
     2101 atgcccggct ggcgcagcga gtcctgcccc gccggtaggc accgccttgg ggccgcacgg
     2161 cgtgaacatc atggagttcg tgaagcagtt caacgcgcgg acccagtcgc aggcaggtat
     2221 ggtgatcccg gtcgtggtca cgattttcgc ggaccggacc ttcaacttca tcaccaagac
     2281 tccgccggca cccaatctcc tcctcaaaga ggcggggatc gagaagggca gcgcccagcc
     2341 taatcgcacc aaggtgggca aggtcacccg ggcgcagctc aagaagatcg ccgagataaa
     2401 gatgcaggat ctcaatgcat cggatctcga tagcgcaatg agaatgattg cggggaccgc
     2461 gcgctcgatg ggcctcgagg tggccgactg atgccggccg tcggaaagaa gttccgggcg
     2521 gcgcaaggca aggtggatcg gactcgacac tacccggttt tggaagccgt aggtctggtc
     2581 aagcagtccg cgtttgccaa gttcgatgag actgtggacg tcgccgtgaa cctcggcgtc
     2641 gatccccgcc acgccgacca ggttgtgcgg ggcacggtgg tcctgcccca tggaaccgga
     2701 aagtcggtgc gagtgttggt tattgcccag ggcgacaaag cgaaggaagc agaagctgcg
     2761 ggggccgatt tcgtcggcat cgagtacgtc cagaagatca aggacggttg gctcgattgc
     2821 gacgtcatcg tcgccacgcc ggacgtcatg ggtcagctgg gccagctcgg acgggtgctc
     2881 ggcccacgcg gactgatgcc gaatccgaag gctgggacgg tcaccctcga cgtcaccaag
     2941 gcagtccgag agatcaaggc gggcaaaatc gagttccggg tcgataagac cggcaacgtg
     3001 cacgctcctg tgggcaaagt ctcgttcaat gcggagcagc tg
//
LOCUS       DASRJQ010000052         2615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_4194642, whole genome shotgun sequence.
ACCESSION   DASRJQ010000052 DASRJQ010000000
VERSION     DASRJQ010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4194642"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VFB89_01260"
     CDS             complement(<1..378)
                     /locus_tag="VFB89_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25959.1"
                     /translation="MSSVDPEALRLIRELQANRASLSPAVGAKVDELVAKYIRPSAQM
                     PTMPIPSPPPIERGLLDEAVGAVSQFWENINPMPQARTFAGHMQERYAQMPGTAVVSP
                     FGHGGAAPGGTIPAIAAMGDTALD"
     gene            complement(375..1748)
                     /locus_tag="VFB89_01265"
     CDS             complement(375..1748)
                     /locus_tag="VFB89_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25960.1"
                     /translation="MSSRDIFAGFIQNVAERSNKQQQDLLQQEMARQQAAAGFWKSMA
                     DNPRVQPAWRDKYLQRYAMILNSPPGKKLPKEASLDMLMEDFALPQRPGPQQAEAPAA
                     VSPALGQTRVDIAAPNPPPGFTGEHPMMGQAQMEAEALRPLRAKLDLETEYARKQFAD
                     QEQIRAMYRAPAVPPDVYASSPLGIFNKFSGQILHPASPDENDRRTVDQKNYAQYAEE
                     TKAAGGKPMSFDAWLTMDANRRRSQTIVGGLAGPVQTRVQSLASAYDNHPTTKQYNEA
                     ALRLANVNTILQGTWSGPGDMAIIFEFMRALDPTSVVRESEYATASKTGNIFAGWAAR
                     FNGALRPEGGFLSDQLKKDFQRVLQGRIQQATRQAQGIYNDFGRRIDRITQQPGTGTG
                     YLTDYTTVFPIDVGQQDRAPSPPSGATPSGGLSEADVLQRLEEIDRRLSQGAKAPAKT
                     PPKGKKK"
     gene            complement(1753..2481)
                     /locus_tag="VFB89_01270"
     CDS             complement(1753..2481)
                     /locus_tag="VFB89_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25961.1"
                     /translation="MPFLGPVAGPLVAKGAISLGGSLLGGKLFGGPSKDEKNILSQMQ
                     AASAQGQRMASGLETQGNELMGMARGAYNPVIDYWTKLLSGGSQAASVLAPEYAQIGQ
                     GYRAANQAASELMPRGGGRASLMQALPYQQMAQQQGLAQTLRPQAASQLGSLAGNVAS
                     SGSNLMSNAVQALYGGTAAGRSALMQMEAQRERGVEMGKQISDIFLNAINSGQIDLSG
                     IFGGKKKSGGGILSSADDIPIRLG"
BASE COUNT          409 a          822 c          825 g          559 t
ORIGIN      
        1 gtcgagggcg gtatctccca tcgccgcgat tgcggggatc gttccgccgg gcgcggcgcc
       61 gccgtgaccg aagggcgata ccacggccgt accgggcatc tgggcgtaac gctcctgcat
      121 gtgcccggcg aaggtccggg cttgcggcat cgggttgatg ttctcccaga attggctgac
      181 ggccccgacc gcttcatcga gcaggccgcg ctcgatcggc ggcggcgacg ggatcggcat
      241 cgtgggcatc tgcgccgatg gccggatgta tttggcgacc agctcatcga ccttggcgcc
      301 gactgccggc gagagtgagg cacggttcgc ctgcagctct cggatcagtc ggagcgcttc
      361 cgggtcgacg gaactcattt cttcttaccc ttcggtggcg tcttcgcggg tgccttcgcg
      421 ccctggctca ggcgtctatc gatctcttcc agacgttgga gaacgtcggc ttctgacaga
      481 cctccgctcg gcgtcgcccc cgacggcgga ctcggcgcac ggtcctgttg ccccacgtcg
      541 atcgggaaca ccgtcgtgta gtcggtcagg tagcccgtcc ccgtgcccgg ctgctgtgtg
      601 atgcgatcga tccggcgtcc gaaatcgttg tagatgccct gggcctgccg ggtggcttgc
      661 tggatccggc cttgcagtac gcgctgaaag tctttcttca actggtcgct caggaatcca
      721 ccttcgggcc gaagtgcgcc attgaaacga gcggcccagc ctgcaaagat attgccggtt
      781 ttactcgcgg tcgcgtactc ggactcacgc acgacggagg tcgggtcgag cgctcgcatg
      841 aattcgaaga tgatcgccat gtcgccggga ccggaccagg tgccttgcaa aatcgtgttc
      901 acgttggcca gccgcagcgc ggcttcgttg tactgtttcg tcgtcgggtg attgtcgtag
      961 gccgaggcga ggctctgcac ccgcgtctgc accggcccgg ctaaaccacc cacgatggtc
     1021 tgggaacgac gacggttggc atccatggtc agccaggcat cgaaggacat cggcttgccg
     1081 ccggctgcct tggtctcctc cgcgtactgt gcgtagttct tctgatcgac ggtgcgccgg
     1141 tcattctcgt ctggtgacgc tgggtgtagg atctggccgg agaatttgtt gaagatcccg
     1201 agcggagaac tggcatagac atcagggggc acggcgggcg cgcgatacat tgcccgtatc
     1261 tgttcttggt ctgcgaactg cttcctcgcg tattcggtct ccagatccaa tttggcgcgc
     1321 agcgggcgta gggcctccgc ctccatctgg gcctggccca tcatcgggtg ctcaccggtg
     1381 aaacccggtg gtgggttggg tgccgcgata tcgacccggg tttgtccgag cgctggactg
     1441 accgcagctg gtgcttcggc ctgttgcggt ccaggccgtt gaggtagcgc gaaatcctcc
     1501 atcagcatgt cgagcgatgc ttccttgggg agcttcttgc ccggcgggct gttgaggatc
     1561 atggcgtagc gttgcaggta cttgtcccgc cacgccggct gcacacgggg attatccgcc
     1621 atcgatttcc agaagccggc agcggcctgc tgccgcgcca tctcctgctg cagcagatcc
     1681 tgttgctgct tattggaacg ctcggcgacg ttctggataa agcccgcgaa aatgtctcgg
     1741 ctgctcatat ggtcacccta gacggatcgg gatgtcgtcc gccgatgaga gtatgccgcc
     1801 gccggatttc ttcttcccgc cgaaaatccc cgataggtct atctggccgc tattgatcgc
     1861 attcaagaag atgtcggaga tctgcttacc catttcgacg ccgcgctcgc gctgggcttc
     1921 catctgcatc aaggcgctcc gtcccgctgc ggttccgccg taaagggcct gcacggcgtt
     1981 ggacatcagg ttgcttcctg acgatgccac atttcctgcg agagagccca gctgcgaggc
     2041 ggcttgcggg cgcaaggtct gcgccagtcc ctgttgctgc gccatctgtt gatatgggag
     2101 cgcctgcatc agactcgcgc gcccgccgcc tcgcggcatt aactcggagg cagcttggtt
     2161 cgctgcccgg tagccttgcc cgatctgagc gtactccgga gccaacacgc tcgcggcttg
     2221 ggatcctccc gacaggagct ttgtccagta gtcaatgact gggttatacg cgccacgggc
     2281 catacccatc agctcgttgc cttgggtctc caagccagac gccatacgct gaccctgcgc
     2341 agatgcggcc tgcatctggg agaggatgtt cttctcgtct ttggagggtc cgccgaagag
     2401 cttgccgccg agcagcgagc cgcccagcga tatcgcgcct ttggcgacga ggggaccggc
     2461 gacaggacct aggaaaggca ttagacgacc tccttacgcc acaccgtgta ggatgtgcgc
     2521 gtcataccgg ccagctcggc catgcgttcc actttgctgt cgtctgtgaa cgcaaaatac
     2581 accaaaccct tacgctcggc cagcgcttcc tcgat
//
LOCUS       DASRJQ010000053         2801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1099456, whole genome shotgun sequence.
ACCESSION   DASRJQ010000053 DASRJQ010000000
VERSION     DASRJQ010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099456"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="VFB89_01275"
     CDS             complement(<1..1003)
                     /locus_tag="VFB89_01275"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HZM25962.1"
                     /translation="MTDAPRLLLLDGHSLAYRAFYALPVENFSTSTGQPTNAVYGFTS
                     MLINVLRDERPTHIVVAFDVSRASFRREQYSEYKANRSETPTDFKGQVSLVQEVLAAL
                     RIPVVEMPGYEADDVIATLTDQARAQGMQVLICSGDRDAFQLVNDDVTVLYPKRGVSE
                     LARMDPAAVAARYGVGPEHYRAIAAMVGETSDNLAGVPGVGEKTAAKWILTYGDFDGV
                     VEHVDQIKGKVGDSLREHLGAVLRNYELNRLVHDLVLPLDPAETAWRGWDREAVHRVF
                     DTLQFRVLRDRLYEYLEAVEPEAESGFDLAGEVLGPGQVRAWLDRHAPVGVPVGVAVA
                     "
     gene            complement(1058..1669)
                     /locus_tag="VFB89_01280"
     CDS             complement(1058..1669)
                     /locus_tag="VFB89_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZM25963.1"
                     /translation="MTEGRSRRVLIAEDEALIRLDLREMLVEEGYEVCGEAGDGEAAV
                     NLAEELRPDLCIFDIKMPIMDGLAAAEKIASGRIAPVVILTAFSQRDLVERARAAGAM
                     AYLVKPFQKSDLVPAIEIALSRFAEMHALEAEVASLSDRLETRKLVERAKGTLMAAFN
                     MTEPDAFKWIQRAAMDNRMTMKQVAEKIILENLPATGSTPASA"
     gene            1812..1888
                     /locus_tag="VFB89_01285"
     tRNA            1812..1888
                     /locus_tag="VFB89_01285"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1846..1848,aa:Leu,seq:caa)
     gene            complement(2162..2500)
                     /locus_tag="VFB89_01290"
     CDS             complement(2162..2500)
                     /locus_tag="VFB89_01290"
                     /inference="COORDINATES: protein motif:HMM:NF023202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsr2 family protein"
                     /protein_id="HZM25964.1"
                     /translation="MARETITRLVDDLDGSEAQETIRFALDGRSYEIDLSAKNAAKLR
                     SALDIYVENGTRAGRASATAGRAARATASSTRNDNQAIRIWAEEQGYDVASRGRISQE
                     IIDLYRKHAR"
BASE COUNT          461 a          930 c          887 g          523 t
ORIGIN      
        1 ctgccaccgc cacacccact ggtactccaa ccggtgcgtg ccggtcgagc caggcgcgga
       61 cctgacctgg gccgagcacc tcaccggcca ggtcgaagcc agactcggcc tcgggctcaa
      121 ccgcctccag gtactcgtag agccggtcgc ggagcacccg gaactgcagc gtgtcgaaga
      181 cccggtggac cgcctcgcgg tcccagccac gccacgcggt ctcggccggg tcgagtggca
      241 gaacaaggtc gtgcaccagt cggttgagct catagttgcg cagcaccgcg cccaggtgct
      301 cgcgcaggct gtcgcccact ttgcccttga tctggtcgac gtgctctacc acgccgtcga
      361 agtcgccgta ggtgaggatc cacttcgccg ctgtcttctc ccccacgccc ggcaccccgg
      421 cgaggttgtc gctggtctcg ccgaccatcg cggcgatcgc ccggtagtgc tcgggcccga
      481 ccccgtaccg cgcggccacg gcggccgggt ccatccgggc cagctcggat acgccgcgct
      541 tcgggtagag caccgtgacg tcgtcattga cgagttggaa cgcgtcccgg tcaccggagc
      601 agatcagcac ctgcatgccc tgtgcccggg cctgatcagt gagggtggcg atgacgtcgt
      661 cggcctcgta cccgggcatc tccacgaccg ggatgcgcag cgccgcgagc acctcctgga
      721 ccaggcttac ctgccccttg aagtcggtcg gcgtttcgct gcggttggcc ttgtattcgg
      781 agtactgctc gcgccgaaac gacgcgcggg agacgtcgaa ggccaccacg atgtgcgtcg
      841 gccgctcgtc ccggagcaca ttgatcagca tcgacgtgaa gccgtagacc gcgttggtgg
      901 gctggcccgt gctggtggag aaattctcga cgggcagcgc gtaaaatgcc cggtatgcga
      961 gcgaatgtcc gtccaggagt agcagcctcg gcgcgtccgt cacggcttgg actctagccc
     1021 tctggtgtga cagcgagacc gaggcgggat gtgaaagtca ggcgctcgcg ggggtgctgc
     1081 cggtcgcggg caggttctcc aagatgatct tctcggcgac ctgcttcatg gtcatccggt
     1141 tgtccatggc cgcgcgctgg atccacttga acgcgtccgg ctcggtcatg ttgaatgccg
     1201 ccattaacgt gcccttggcc cgctccacca gtttgcgggt ctccagccga tcgctcaggc
     1261 tcgccacctc ggcctcaagc gcgtgcatct ccgcgaaccg ggacagcgcg atctcaatgg
     1321 ccggcaccag gtcgctcttc tggaacggct tgaccaggta tgccatcgcg cccgctgccc
     1381 gggcccgctc gaccaggtcg cgctgcgaga acgcggtaag tatgacgacg ggtgcgatcc
     1441 gcccgctggc aatcttctcg gccgccgcca gaccgtccat gatcggcatc ttgatgtcga
     1501 agatgcacag gtcgggcctg agttcctcgg ctaggttcac cgccgcttcg ccgtcacccg
     1561 cttcgccaca gacctcgtag ccctcctcca cgagcatttc gcgaaggtcg agccggatca
     1621 gtgcctcgtc ctcggcgatg agaacccgcc tgctccgccc ctcggtcacg gccaacccct
     1681 cgacatgatc atgaatctgt ggtgcggtgc atgctcttta catacggccg cggagccgga
     1741 atcgctcacc cggagtgcac gttcctcgca tgccgcgtca ttagcctaac gggtaggctc
     1801 gtacgcgcta cgtccccgta ggccaaccgg cagagccacg gagctcaaac ctccgccagt
     1861 gtgggttcga atcccaccgg gggcaccaga tcgctgttgc cacgcccagc cggacggaac
     1921 cacggtccgg ggcagtgtgt ttggcttctt ggtgtgatca gcgtgatcgt ttgttaccag
     1981 tgccgcaagc gacgtcgtgg cgcccgtgtt ggcgcgcgag cagaagcgtc cgaggagaag
     2041 cgggagcgga tgtccatgtc ggaatgccgt tacggtcagc cgacatggac atcaccccaa
     2101 ccacacatgt ggacagctac cttgcgcaca gcccaagcca gcctcatcac cgccgccgtg
     2161 attagcgtgc gtgcttccgg tataggtcga tgatttcctg gctaatccga ccgcgggatg
     2221 cgacatcgta cccctgctcc tcggcccaga ttcgaatggc ttggttgtcg ttgcgggtgg
     2281 agctagcggt tgcgcgtgcg gcgcggccag cagtggcgga agcgcgaccc gcccgggtgc
     2341 cattctcgac gtagatgtcg agcgcactgc gcagctttgc cgcgttctta gcggacagat
     2401 cgatctcgta ggaacgcccg tccagagcaa acctgatcgt ctcctgtgcc tcgctgccgt
     2461 ccaggtcgtc aacgagtcga gtgatggtct ctcgtgccac gatgtctcct tttggagggt
     2521 gtaaccggaa tgccggtcga atggtgacgt ccattctagc catatttcag ttgacgcgct
     2581 gactgagtgc ggtacttcac gcagcggcca tatgccgaga gcacgtgcgc atcaatctcc
     2641 gcggagtagc agctcgaacc taaatggact cggccactat tactcttctc cccatttgaa
     2701 gccgccaata ggtggtcggt ccggaacgac ggagacgacg gtcgattcga tcgcggccgg
     2761 tagagtgcgg gggtgactcg atcgggcagc gggaagggcg c
//
LOCUS       DASRJQ010000054         3082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3349094, whole genome shotgun sequence.
ACCESSION   DASRJQ010000054 DASRJQ010000000
VERSION     DASRJQ010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3349094"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="VFB89_01295"
     CDS             <1..1087
                     /locus_tag="VFB89_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25965.1"
                     /translation="RQVDTVIDLDGRWVVPPYADAQQPLIEPGNIATYQRLFLHDGVF
                     YVQDQGAAPMLRARIDSAVNTPMSFDLRSANQGWTGPGGYAVDFAQRAARLGLYTAEW
                     LRDSLDGGVLMLVSSADDVERRWPSFASSRPRPDYVKLYLVYSEQYARRKEGLDPALV
                     GPIVRRAHAAALPVAAHVYTAADFRNAVAGGVDVIVHVPGGSDLPVEAFRLTDMDAEV
                     AAGRRVAVITALGGLADQLRRDSAGTADRIRDVYAPNVDVLRRHGVRLLVGSDVIRQS
                     AAVEIRVLGRMGMFSSLELLKMWSVATPQALFPRRKIGELLDGYEASFLVLAGNPLKD
                     FANTQRIVRRVKQGRTLDLPPENLSVP"
     gene            1084..2055
                     /locus_tag="VFB89_01300"
     CDS             1084..2055
                     /locus_tag="VFB89_01300"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HZM25966.1"
                     /translation="MRAIRFHALGGPEVLQLDEVPKPVPQSGEVLIQVEAIGVNFIDT
                     YQRAGLYKVDLPFTPGQEVAGRIAAIGPGVTEFAEGDRVACYHPGLGGYAEFSAAPAE
                     RVIKLPGGVSAVQGAALMLQGMTAHYLATTTFPLKAGDRCLVHAAAGGVGLLLCQIAK
                     RRDALVFGTVSTEAKAVLALVAGANEVIRYTEQDFELEIQRLTGGVGVQVVYDSVGKT
                     TFEKGLRCLARRGMMVLFGQSSGPVAPIDPQLLNQRGSLFLTRPTLAHYVATRADLEQ
                     RSGDLLHWVLDGTLQLRVEHEFPMAEAAEAHRELAGRLTTGKVLLVP"
     gene            complement(2060..>3082)
                     /locus_tag="VFB89_01305"
     CDS             complement(2060..>3082)
                     /locus_tag="VFB89_01305"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HZM25967.1"
                     /translation="RAAGIVLVVLIHTSGVVAGPGPAGGTTAFWAGLIYDSLARPGVP
                     LFVMLTGWLLLDPKRPPEPIGNFLWRRFGRVALPLLVWSLIGYGWLAIRDHHAMKLAQ
                     FPRQVLNAPVFYHLWYVYVLLGLYLVVPLLRPLVAAASDSLRRYALALWVLGSSVLPL
                     WYWWGGLHIGIDIPVVTTYVGYLLAGVWLAGAPVSARTRRTLGLLILVVVIWTVFATA
                     RLTGDEATNVALFAYGTPNVLAMSVAWFVLLTQPELTARVTRHPRAVTLVRVLAATSY
                     GVYLVHPLILNLLDSGVLGTQITGASMYPFVGIPLLAAAVLAVSVGLMALARRVPMLD
                     RLVGAS"
BASE COUNT          544 a          994 c         1062 g          482 t
ORIGIN      
        1 ccggcaggtg gacaccgtca tcgacctgga cgggcgatgg gtcgtcccgc cgtatgccga
       61 cgctcaacaa ccgctgatcg agcccggcaa catcgcgacc taccagcggc tgttcctgca
      121 cgatggcgtg ttctacgtcc aggaccaggg cgcggcgccg atgctgcgcg cccggatcga
      181 tagcgcggtg aacactccga tgtcgttcga cctccggagt gccaaccagg gctggaccgg
      241 gcccggtggc tacgccgtcg acttcgcgca gcgagcggcc cggctgggtc tctacaccgc
      301 ggagtggctg cgcgacagtc tcgatggcgg agtcctgatg ctggtctcga gcgctgacga
      361 cgtggaacgg cgctggccct cgttcgcgag ctcgcggccc aggcccgact acgtcaagct
      421 gtacctggtc tattccgagc aatacgcccg ccgcaaggaa ggcctcgatc cggcgctcgt
      481 cgggcccatc gtgcgtcgtg cgcatgccgc cgccctcccg gtcgcggccc acgtgtacac
      541 cgcggccgat tttcgtaatg ccgtggctgg gggcgtggat gttatcgtgc acgtgccggg
      601 cggtagcgat ctgccggttg aagcgttccg cctcacggat atggacgccg aagtcgccgc
      661 cggacgccgg gtcgccgtaa ttacggcgct cggcggactc gcggatcagc tccggcgcga
      721 cagcgcgggc accgcggatc gcatccggga tgtgtatgca cccaatgtcg acgtgctgcg
      781 aaggcatggc gtccgtctgc tcgtgggcag cgacgtgatc cgccagagcg cggcggtcga
      841 gatccgcgtc ctgggccgga tgggcatgtt ctcgagcctc gagctgctga agatgtggag
      901 cgtggcgacg ccgcaggccc tgttcccgcg ccgcaagatc ggagagctat tggatgggta
      961 cgaggcgagt ttcctggtcc tcgcgggcaa ccccttgaag gacttcgcca acacgcagcg
     1021 catcgtgcgg cgcgtgaagc agggccgtac gctcgatctc cctcctgaga acctgagcgt
     1081 cccatgagag cgatccgctt tcacgccctg ggtgggccgg aggttctcca gctcgacgag
     1141 gtgccgaaac cggtccccca gtccggtgag gtgctgatcc aggtcgaagc gatcggcgtg
     1201 aacttcatcg atacctacca gcgggccggt ctctacaaag tcgatttacc gtttacgccg
     1261 ggacaggaag tggccggtcg gattgcggcg ataggccccg gagtgacgga attcgccgag
     1321 ggagaccgag tcgcctgcta ccatcccgga ctcggcggat acgccgaatt ctcggctgca
     1381 ccggccgagc gggtgatcaa gctgccgggc ggcgtgagcg cggttcaggg cgccgccttg
     1441 atgctgcaag ggatgaccgc gcactacctc gccacgacca ccttcccgct gaaagccggg
     1501 gacaggtgcc tggtacacgc ggccgcgggc ggcgtgggcc tgctgttgtg ccagatcgcc
     1561 aagcggcggg atgccctggt tttcggcacg gtgtcgaccg aggccaaagc ggtgctggcg
     1621 ctggtcgcgg gcgccaacga agtgatccgc tacaccgagc aggatttcga gctcgaaatt
     1681 cagcgcttga cgggcggcgt cggggttcag gtggtctacg attcggtagg aaagacgacg
     1741 ttcgagaaag gcctgcgctg cctggctcgt cgcgggatga tggtgttgtt tggacagtcg
     1801 agcggtcccg tggcaccgat tgatccccaa ctgctcaatc agcgcggctc gctgtttctc
     1861 acgcgtccca cgctcgcgca ttacgtcgcc actcgcgccg acctggagca gcgcagcggc
     1921 gacctgctgc actgggtcct ggatggcacg ctgcagctgc gggtcgagca cgagttcccc
     1981 atggccgaag ccgctgaggc ccatcgcgag ctggcgggcc gtctcacgac cgggaaggtg
     2041 ttgctggtgc cgtgatgctc taggaagcgc ccacgagccg gtccagcatg gggactcggc
     2101 gtgcgagggc catcagcccg accgacaccg ccaaaaccgc ggctgccaag agcgggattc
     2161 ccacaaacgg gtacatcgat gctccagtga tttgagtgcc caataccccg ctatccagca
     2221 gattcaggat cagcgggtgc accagataga cgccgtagct cgtggcggcc agcacccgca
     2281 ccagggtcac cgcgcggggg tgccgggtca ctcgggccgt cagctccggt tgcgtcagca
     2341 ggacgaacca cgccacgctc atcgcgagga cgttgggtgt gccgtaggcg aacaacgcga
     2401 cgttggtcgc ctcgtccccc gtcagacggg ccgtagcgaa cacggtccag atcacgacta
     2461 cgaggatgag caggccgagt gtgcggcggg tccgcgcgga aaccggagcc cccgccaacc
     2521 agactcccgc gagcaggtag cctacgtagg tcgtcacgac gggtatgtcg atgccgatgt
     2581 gcaggccacc ccaccaatac cagagcggca gcacggagct tcccaatacc cagagggcga
     2641 gggcgtagcg ccgcagcgag tccgaagcgg cggctacgag cggccggagc agcggcacga
     2701 cgagatacag ccccagcagc acgtagacgt accacaggtg ataaaacaca ggggcgttca
     2761 ggacctggcg cgggaattga gccagcttca tcgcatggtg atcgcggatc gccagccatc
     2821 cgtagccgat gagggaccag accagcaagg gcagggcgac acgcccgaat cgtcgccaca
     2881 ggaaatttcc aatcggctcg ggcggacgtt tggggtcgag cagcagccag ccggtgagca
     2941 tcacgaacag gggaacaccc ggccgcgcga gactgtcgta gatgagtccg gcccagaagg
     3001 ccgtcgtccc tccggccgga cccggaccgg cgacgacacc ggatgtgtga atgaggacga
     3061 cgagcacgat gcccgcggcg cg
//
LOCUS       DASRJQ010000055         6105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1096979, whole genome shotgun sequence.
ACCESSION   DASRJQ010000055 DASRJQ010000000
VERSION     DASRJQ010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6105
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096979"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2413)
                     /locus_tag="VFB89_01310"
     CDS             complement(<1..2413)
                     /locus_tag="VFB89_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25968.1"
                     /translation="MRRHIARFVFVVGLGSIAAVLGVVTALLLTPPGRDLLARLVSRE
                     LSRVLLGSVEVGSVSGSFLYDLTIDNLVIRDTSGVLLADLPRVRVGYRVPNFFARRFV
                     LGRLQVDRPTIQLLKHRGGRMNYEEVLRLGGGKGGGPSPLIEFRDVQVREGTLRLMLP
                     WSPSPQLRTEEQRDSALAAERSRPGKVIESGPEGLRRVIDVTDLTARLDRMRISTPDR
                     QPFTIDIDTLAARVSDPQLTIRDAAGRVRLRGDSVVFSLRRGALPHSRFAGGGAVTWP
                     HDTTLYDLQVVAPQASLADLRWISANFPDLTGRAVVAARSENGTRTAFVVTNLHLVRG
                     PTRVDGQLTVLDDRKRGLGVRDLRLTHTALDLDEVRPFMPDLPFYGTLTGTTIATGFL
                     DALDARMDWDFTDDEVVGRPVTHIVGKGQISARPGQGLAFHGLEITESDIDFGTVRRL
                     APAVILQGRLAAQGTLEGPLRNVTFNGTARHYDGNSPSSELEGMVHLDTRRDTLALAT
                     DVALQPLVFDGIRRSFPALTSTGSITGRVRLQGTLARLTVDASVSGEIGTIEAKGGAT
                     VQPPRWGARDLALQFQRLDLAALRGSGPTTSLNGSLSVTGETDTARAPEADLDLVLGP
                     GRVRDLRLDTLVARGSVHDSLIKVDTLEGRLASVTARGRGTLGWARPHEGRMSFDLQT
                     DSLGIFDSLLTVWTGLTRDTSRDAVPLTGTAQGSLTLGRSLDSLDVAASFAGKKLAWS
                     TLRAPVAIGAFTYVGGRRPRMTAAFGIDSLAMRERVFRRLGLQARGFTDSLEWNGAVA
                     IGNEVA"
     gene            complement(2413..4536)
                     /locus_tag="VFB89_01315"
     CDS             complement(2413..4536)
                     /locus_tag="VFB89_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HZM25969.1"
                     /translation="MRASAYALLVGIAMVRSLAAQQPDRVVRRLDFEGAKAIQRTTLE
                     AAIGTTNSSWFATFPLVRWIGLGAKRYFNETEFRRDILRLQVLYKRSGFPDVAVDTVV
                     RRTDTNVYITFRIKEGRPIRITKITILGVDSLRQRAQERLLLDLPLREGDVFNRYVMQ
                     TSADTITSRLKNHGYPEAAVFTSFESNRTEYTANVTFDVDPGPRAPFGQVRVMGTQRL
                     DSTIVPDLLVARTGRPYSRDLMFQSQRNLYKTDLVRSATVDIDSARYTPGDSVPILVR
                     LAESPPYRLRTGLGYATTECFRAQAAFAVRDFFGHGRLFELTGNVSRIGVGDPFDFGF
                     GNNICSVAAEDSIGSARFSYQLAATIRRPAFVSPNNTLSLTAFAERRSEFKVYVRSDI
                     GSTVEILHETPRRRFPVSLAYTISYGRTEATPASFCAFFNACTPDVIDQLEQRRLLAA
                     ITGRLSIPRANSPIDPTRGSNAVLELVWASKLIGSSEFQQFTRVLGDVAWYRPLARGV
                     VLTWHLRGGMIFAPEVALATGTNAFVPPEQRFYAGGPNDVRGFPRNQLGPVVYVVTET
                     ALASVHDSTSLLPPDSVQVAPTGGNTLVGGNIELRFPSPVLGTRMRLALFVDGGMLWE
                     RGRPDLAPAALRITPGAGLRVGTPLGPARLDVAYNPYSALRGTLFQTDSAGALTKVQD
                     NFAVDKRSFFLGIPLTVQFSVGQPF"
     gene            4730..>6105
                     /gene="dacB"
                     /locus_tag="VFB89_01320"
     CDS             4730..>6105
                     /gene="dacB"
                     /locus_tag="VFB89_01320"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HZM25970.1"
                     /translation="MRIHIPILRSLLLRADRSRRGGTVRLSIAITALIVLLAAPLLGQ
                     APASARSHPLRPPPVKALARRLNHRLDQPPLDRFLWGISIVDDGGRRLYERNANRLFV
                     PASTAKIVVSTVAAALLPADSGVRTGLYATGPLVNGILQGDLVLYGRGDPTLSRRCYA
                     ADTTRASACEPDAFAPLRRLAVQLRSQGVQTIAGDLVGDGSWFEPTLVHPAWEGYDLN
                     WWYAAPVSGLGFNDNSVDLTWGAGPAIGGPATVSFTPALDDVTLENHAVTIPGDTGAT
                     IDFFRDPGTMRIRAEGNVPQGSSGKTEHFALPDPNLFAAWAFASVLHEAGIAVNGQVR
                     STTDSSAYRAARAERPLAEFESRPFRDWIFPILNSSQNWFAEMVLKQLGRRFGRAGSW
                     HDGLTLERRFLIDSVRIDSTQFALVDGSGLSATNSITPDALIRVLQFARRHPHMEAFL
                     DALPRSGER"
BASE COUNT         1102 a         1989 c         1999 g         1015 t
ORIGIN      
        1 atgccacctc gttgccgatg gctacggcgc cgttccactc gagtgaatcg gtgaaaccgc
       61 gcgcctggag accgagccgc cggaacactc gctcgcgcat cgccagggag tcgatgccga
      121 aggccgcggt catgcggggg cggcgaccac cgacgtacgt gaacgcgccg atcgcaaccg
      181 gcgcccggag cgtgctccac gccagcttct tgcccgcgaa ggaggctgcc acatccagcg
      241 agtcgagact ccgtcccagc gtgagcgagc cttgcgccgt accggtcagc ggaactgcat
      301 cacgcgaggt gtcccgcgtc aaccccgtcc acacggtaag cagcgagtcg aaaatgccta
      361 ggctatcggt ctggagatcg aacgacatcc gtccctcgtg aggacgggcc cagccaagcg
      421 ttccccggcc ccgcgcggtg acgctggcga gccggccctc gagcgtgtcg accttgatga
      481 gactgtcgtg cactgagccc cgggcaacca gtgtgtcgag tcgtagatcg cgcacgcgtc
      541 cgggccccag aaccaggtcg agatccgctt ccggagcgcg ggccgtatca gtctctccgg
      601 tgacactaag cgaaccattc aggcttgtgg tcgggccgct gccgcgcaac gccgcgagat
      661 cgagtcgctg gaattgcagt gcgagatcgc gcgctcccca acgcggcggc tggaccgtcg
      721 cgccgccctt cgcctcgatc gtcccgatct caccgctcac cgacgcgtcc accgtcagac
      781 gagccagggt tccctggagc cgaacccgcc cggtgatgga ccccgtgctg gtgagcgcgg
      841 ggaacgagcg acggatgcca tcgaacacga gcggctgtag cgcgacatcg gtggcaaggg
      901 ccagggtatc gcgccgggta tcgagatgaa ccattccttc cagctcgctc gaaggcgaat
      961 tgccgtcgta atggcgcgcc gttccgttga aggtcacgtt ccgaagcggc ccttcgagtg
     1021 tgccctgggc tgccaggcga ccctgtagaa tcaccgccgg ggcgagccgg cgcaccgtgc
     1081 cgaaatcgat gtccgattca gtgatttcga gtccgtgaaa ggccaacccc tgcccaggcc
     1141 gcgcgctgat ctgacctttc ccgacgatgt gggtcaccgg gcgccccacg acctcgtcgt
     1201 cggtgaaatc ccagtccatc ctcgcgtcga gcgcatccag gaatcccgtg gcgatcgtcg
     1261 tccctgtgag cgttccgtag aagggcaggt ccggcatgaa ggggcgaact tcgtccagat
     1321 ccagcgcagt atgggtgagg cgaagatcgc gaaccccgag tccgcgcttc cgatcgtcca
     1381 gaaccgtcag ctgtccatcg acgcgagtcg gcccgcgcac gagatggagg ttggtgacga
     1441 cgaaggcagt tcgggtgccg ttttccgagc gagccgccac gaccgcgcga cccgtgaggt
     1501 ccggaaagtt ggccgagatc cagcgcagat cggccaggct ggcctggggc gcaacgacct
     1561 ggagatcgta gagcgtcgta tcgtgaggcc aggtgaccgc acctccgccc gcaaaccggg
     1621 agtgcggcaa agcgcctcgg cggaggctga acacgacgct gtcgccccgc aggcgcacgc
     1681 ggccggccgc gtcgcggatg gtgagttgtg gatcgctcac ccgggccgca agtgtgtcga
     1741 tgtcgatggt gaagggctga cgatccgggg tgctgatccg catgcggtcg agcctcgcgg
     1801 taaggtccgt gacatcgatg accctgcgca gcccctccgg tccggattcg atcactttgc
     1861 cgggacgact gcgctcggca gcaagcgccg aatcacgctg ctcctcggtg cggagctggg
     1921 gcgagggcga ccagggcagc atgagccgca gtgtcccctc ccgcacctgc acgtcgcgga
     1981 attcgatgag gggtgacggg cctcccccct ttccccctcc cagccggagc acctcctcgt
     2041 agttcatgcg tccgccgcgg tgcttgagca gctggatcgt gggacgatcc acctgcagtc
     2101 gaccgagcac gaatcggcgg gcgaagaaat tgggcacccg gtagcccacg cgcacccggg
     2161 gcagatcggc cagaaggacg ccgctggtat cccggatcac gagattgtcg atggtaaggt
     2221 cgtacaggaa tgatcccgac acggatccga cttcgacgga gccaagcagc acacggctca
     2281 gctcgcggga gacaagacgc gcgaggagat ctcgacccgg aggagtgagc aggagagcgg
     2341 tgaccacacc caacaccgcg gcgatcgacc cgaggccgac gacaaagacg aatcgcgcga
     2401 tgtggcgacg catcagaacg gctggcccac cgagaactgc acggtcagcg gaatccccaa
     2461 gaagaaggaa cgtttatcca ctgcgaagtt gtcctgcacc ttggtgagtg cgcccgccga
     2521 gtcggtctgg aagagggtgc cgcggagcgc cgagtatgga ttataggcca cgtccagccg
     2581 ggcgggcccg agcggtgtcc ccactcgaag tccggcgccg ggggtgatgc gaagcgcggc
     2641 cggtgcgaga tcgggcctgc cccgttccca cagcatcccc ccatccacga agagggccag
     2701 ccgcatccga gtgccgagca ccggcgacgg gaagcgcagc tcgatgttgc caccgacgag
     2761 cgtgttgccg ccggtcggcg cgacctgcac cgagtccggc ggcaagagtg acgtcgagtc
     2821 gtgcacactg gccagtgcgg tttcggtgac gacgtatacc accggcccca actgattgcg
     2881 ggggaacccg cgcacatcgt tcggtccacc cgcatagaac cgttgctccg gcggaacgaa
     2941 ggcattggtc ccagtggcca acgcgacctc gggtgcgaag atcatgccgc cgcgcaggtg
     3001 ccaggtcagc accaccccac gtgccagcgg gcgataccag gcgacgtcgc ccagcacccg
     3061 ggtaaactgc tgaaattcgg acgagccgat gagcttcgag gcccacacca gctcgagcac
     3121 ggcgttggat ccccgggtcg gatcgatcgg gctgttggct cgcgggatac tcaatcgccc
     3181 ggtgattgcg gcgagcaggc ggcgttgctc gagctgatcg atgacgtccg gtgtgcaggc
     3241 gttgaagaag gcacagaagc tcgccggggt cgcttccgtc cggccatacg agattgtgta
     3301 ggcgagggac accggaaacc gccgcctcgg tgtctcgtgc aggatctcca ccgtcgagcc
     3361 gatgtcactc cggacgtaca ccttgaactc cgagcgtcgc tcggcaaatg cagtgaggct
     3421 caaggtgttg ttcgggctca cgaaggcggg ccggcggatg gtggcggcga gctggtagct
     3481 gaatctggcc gagccgatcg agtcctctgc ggcgacgctg caaatgttgt tgccgaatcc
     3541 gaagtcgaag ggatcgccga cgccgatccg ggacacgttg cctgtcagct cgaacaatcg
     3601 gccatgcccg aagaagtcac gcactgcaaa ggcggcttgc gcccggaagc actcggtggt
     3661 ggcgtagccc aggccagttc gcagccgata gggcggactt tcagccaacc gcaccagaat
     3721 cgggaccgaa tcgcccgggg tatagcgcgc cgaatcgatg tcgacggtag cgctccgcac
     3781 cagatccgtc ttgtagaggt tccgctggct ctgaaacatc aggtccctgg agtacggccg
     3841 cccggtgcgc gcgaccagga ggtctggaac gatggtggaa tcgagccgct gtgttcccat
     3901 aacccgaacc tgtccgaagg gcgcacgggg ccccggatcg acatcgaacg tgacgttcgc
     3961 ggtgtattcc gtgcggttgc tttcgaagct ggtgaagacg gcggcctcag gatacccgtg
     4021 gttcttgagt cgggacgtaa tcgtgtcggc cgaggtctgc atgacgtagc gattgaacac
     4081 gtcgccttcg cggagcggca ggtcgagcag cagccgttcc tgcgcccgct gccgaaggga
     4141 gtccactccc aggatcgtga tcttcgtgat gcggatcggg cggccttctt tgatacgaaa
     4201 cgtgatatac acgttggtgt cggtgcgccg caccacggtg tcgaccgcaa catcaggaaa
     4261 gccactgcgc ttatacagca cctggagtcg caagatgtct cgccggaact cggtctcgtt
     4321 gaaataccgc ttggcgccaa ggccaatcca gcggaccaga ggaaacgtcg cgaaccagct
     4381 cgagttggtc gttccgatcg ccgcttcgag ggtggttctt tgaatcgcct tggcgccttc
     4441 gaaatcgagt cgacgcacga cgcggtcggg ctgttgggcg gcgagtgatc ggaccatcgc
     4501 aatcccgacc agcaaggcgt acgcactggc tctcaatagt ccgaacccgg gccgggtgcg
     4561 gcaaaatcgg tgggtcgaaa tgcagcactc cggaggagga aggaccggcc tccgcacccc
     4621 cggccggcaa ttgacgtcac cctctaagat gggtagtttg acggccttac gttgacgtgt
     4681 caacgttggc cggcagccaa acgcattcac cggtcgccgg ctcccgccca tgcgaatcca
     4741 catcccaatc ttgcgttccc tactattacg cgccgaccgg tcgcgccgcg gcggaacggt
     4801 tcgactatcc atcgcgatta ccgcactgat cgtcctgcta gcggctccgc tgctcggtca
     4861 ggctcccgct tcggctcgct cgcatcccct tcgcccgcct ccggtcaagg cgctcgcccg
     4921 gcgactgaat caccgtctcg accaaccgcc gctcgatcga ttcctctggg gcatctcgat
     4981 cgtggacgat ggaggacgcc gactctatga gcgcaatgcg aaccggctgt tcgtgccggc
     5041 cagcacggca aaaatcgtgg tcagcacggt ggcggcagcg ctgctccctg cagacagcgg
     5101 agtgcggaca ggtctctacg cgaccgggcc gctagtgaac ggcatcctgc aaggcgacct
     5161 ggtgctctat gggaggggcg accccaccct gagccgacgc tgttatgccg cggacaccac
     5221 gcgagcctcg gcctgcgagc ccgacgcatt tgctcctttg cgtcggctcg ctgtccaact
     5281 ccggagtcag ggtgtccaga ccatcgcggg ggatctcgtg ggcgacggga gctggttcga
     5341 gccgactctg gtccatcctg cctgggaggg ctacgacctg aactggtggt acgccgcgcc
     5401 ggtatccggc ctcggcttca acgacaacag cgtggatctc acctgggggg ccgggcccgc
     5461 gatcggcggg cccgcgaccg tttcattcac ccctgctctg gatgatgtca cgctggaaaa
     5521 tcacgcggtc actatcccgg gcgataccgg tgccacgatc gacttctttc gtgatcccgg
     5581 gaccatgcgg atccgagccg aaggaaacgt gccccaggga agcagcggaa agaccgaaca
     5641 tttcgcgctg ccggatccga atctgttcgc ggcatgggcg tttgcctcag tgctccacga
     5701 ggccgggatc gccgtcaacg gccaggtgcg ctcgactacg gactcatcgg cttaccgggc
     5761 tgctcgcgcc gagcgccctc tcgcggagtt cgagtcacga ccgttccgcg actggatctt
     5821 tccaattctc aatagcagtc agaactggtt cgctgaaatg gtgctcaaac agctgggccg
     5881 ccgcttcggg agggccggct cgtggcatga cgggctcacg ctcgagcgcc ggttcctgat
     5941 cgattcggtc cgcatcgact cgacccagtt cgcactggta gacggctccg gcctctcagc
     6001 gaccaattcg ataacgccgg acgcactgat tcgcgtgctc cagttcgccc gtcggcatcc
     6061 gcacatggag gcgtttctgg acgcccttcc caggtcgggc gagcg
//
LOCUS       DASRJQ010000056         9042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1370080, whole genome shotgun sequence.
ACCESSION   DASRJQ010000056 DASRJQ010000000
VERSION     DASRJQ010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 9042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9042
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370080"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="VFB89_01325"
     CDS             complement(<1..604)
                     /locus_tag="VFB89_01325"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZM25971.1"
                     /translation="MTTFGRAPCDEGVIQAGPRAVADCEEKAKPWVLATTILGSAMAF
                     IDGSVVGVALPAIQADLNASVRETQWVVNAYMLMLGALILVGGAAGDRFGRRMMFILG
                     VTTFTIASVACGFAPDAFSLTVARAAQGIGGAILVPSSLAIISATFPEKERGRAIGTW
                     AGASALTTALGPVLGGWLVDAFSWRAIFFVNVPIALLAVFI"
     gene            755..1807
                     /locus_tag="VFB89_01330"
     CDS             755..1807
                     /locus_tag="VFB89_01330"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZM25972.1"
                     /translation="MQLRTLIFFVTSRCNAKCRTCFYWQELNRPGDLTFDEIATLTRT
                     MPRFRELWLSGGEPMMRPRLADIIQLFYQRNEIRTLNLPSNGLFRDRLVRLMDFITAS
                     LPKLKVNLNLALDGFAETHDRIRGVPGNFDQAIRTIEALYPVRDRNPNIRIHVNSVIT
                     AENIDELEPLGRWLIEHARLNGQYFQVVRGDPLDPALKSVGPERLAEFYRRVEPLHRH
                     YGRYLSQRHGGGPKGRLAEFYYNRTLSFYYEVQHANLEQSTRWPMPCTAGQSILVVDY
                     NGDVRACELRQKIANLREVGCDFRRVLHSEALSRETAGIVRDQCWCTHVCFIHDSAKS
                     SRRAQLFEIPFKRHQV"
     gene            1808..2887
                     /locus_tag="VFB89_01335"
     CDS             1808..2887
                     /locus_tag="VFB89_01335"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZM25973.1"
                     /translation="MTLPLVLLWGAAVCYTLVVTAISARLVGAALPGPPWIVEQIVLL
                     LFWAAATPAILWSARLFPLDRRGRRLGHLGVHLLFAFAFILATNLVAPVLTRLILGQS
                     FDVLAVMRLGLLDFLQVYHLALIVYAFIVGVGHYLPAREAHRAQQLRAERLRASLAEA
                     RLRALQLQLEPHMLFNALNAIGALVITGKRDEAFDAIGRLGDLLRAVLATESQQEVSL
                     REELRLTESYLAIERARLGDRLDARWEIAPGIEMARIPPLLLQPLVENAVRHGIARKV
                     EGGRVVVRADRVGERLRLEVRDDGPGVSEGAASGSGIGLENARRRLEQLYGPEHRLDL
                     ERTDDWTRVSLELPYHGMDERSEAA"
     gene            2884..3603
                     /locus_tag="VFB89_01340"
     CDS             2884..3603
                     /locus_tag="VFB89_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HZM25974.1"
                     /translation="MTLRVVLVDDEPLARAALAAILEDQPDVEIAGESASAADAVRVV
                     RTLEPDLVFLDIALPDHDGFWVLEQLAGRQPAIVFVTAHSDRAVKAFEVRALDYVTKP
                     LRRHRVEEAVARARERTSPPPDGRRAAGGPLTLKTDGTLRLIDQDTIDWIAADDDHIV
                     VHASGGRWRARETLQEVERRLDPRRFVRIHRSTVVRLNRVRELQPWFHGDYVAILHTG
                     AKLRVSRTYRERVARFLGRDL"
     gene            3723..5561
                     /locus_tag="VFB89_01345"
     CDS             3723..5561
                     /locus_tag="VFB89_01345"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZM25975.1"
                     /translation="MRRIIGGLLLGWLVLCRDVAAQNGSLMFRGDPAHTGVSTAAFFQ
                     GQGGVRWRVRTGDAVRSSPAVTQTRVFAGSGDGYLYAIDRANGKVVWRYAAGGPVHSS
                     PAIAGGLVVAETMDGRIFAVDQKSGKQRWSMKTGPALPFNTFPAGAWGYIASSPVVVG
                     QRVVIGAPDGGVYALDLTSGKQRWHTRTNGRVRATPAVVDGVVVVGSFDGRVYALDLE
                     TGVERWVHKTEGDTLDSAKWGFDRRAIQGSAAVSDGTVYVGSRDSGLYAIDFATGHRR
                     WRFSHRGSWVLAAPSVRDGKVFVGSSDGHFFQGVDSSGKELWRLPTDANMLGSPLLIG
                     NTLLAGTYPRDTPNGELLAIDAGTGNVRWRLNLDASVLSSPVAFDNEIYLGTEDGSIV
                     AVGQASPTIPRLAVFYDSTLGGNAFAKGGALAFAYFRDLGYQPLGADSLAAFLNARIA
                     DSVPSAVVFATDVLPSNVAPTPSDTTLFRRYLNAGGKVVWIGGPIDVFVHDSTGQLVG
                     FEVKRMADLLGISVDSLDFNEYTAHPTDAGRRWGIDRALRGATPMARSAVSEALAVDE
                     TGMTTAWVRHYRTDRLWSGFVQLWGLGASWDRLPAIRAVAEYGLLR"
     gene            5652..7067
                     /locus_tag="VFB89_01350"
     CDS             5652..7067
                     /locus_tag="VFB89_01350"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HZM25976.1"
                     /translation="MAIGASFLEQVNRSFDRAASFTGHDATLLANIKACKNLFYTSFP
                     IKLDNGRIEVMHAWRAEHSHHKLPCKGGIRYAADVDADEVQALAALMTYKCALVDVPF
                     GGAKGGIRLNPKKYSEGELERITRRFVFELVRKNFIGPGLDVPAPDVGTSPREMAWIV
                     DTYSQLRSGELDALGCVTAKPLSQGGIRGRVEATGRGVFYGVREVVSIAEDMKPLGLS
                     PGIAGKRVVIQGMGNVGYFAARFFQSGGAVVVGLAESEGAISNPKGLDVDQVMVHRKE
                     CGGILGFPGATNLARREDALELECDILIPAALERQITAENAPKVKAKLVVEAANGPTT
                     SEADEILGRRGITVIPDAYINAGGVTVSYFEWVKNLGHVRFGRMQKRFEQGAYGRLLE
                     AVEGITGRSFTPAEIERLTHSADEEDLVNSGLEETMTTSYHPIREVWKQHGGKVDLRT
                     AALIVAINKVAVSYEQLGIFP"
     gene            7113..8345
                     /locus_tag="VFB89_01355"
     CDS             7113..8345
                     /locus_tag="VFB89_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZM25977.1"
                     /translation="MSNDQQVPRTAFAVLGAISFCHLLNDMMQSLLPAVYPMLKASFA
                     LDFGQIGLITLTYQLVASLLQPVIGHYTDQRPQAYSLAVGMAFTLIGLLVLAHAGGFA
                     TILAAAGLVGVGSSVFHPESSRVARIASGGRLGFAQSLFQVGGNAGSSLGPLVAAFVV
                     VPRGQASIGWFSVAALLGIIILWNVGRWYGERHGPRATKEWVAHQPATSTLPSKQVRI
                     ALAVLIALIFSKYIYLSSLISYYTFYLIHKFHLSVQSAQIYLFAFLGAVAAGTIIGGP
                     VGDRVGRKYVIWCSILGVLPFTLALPYANLFWTGVLSVLIGLILASAFSAILVYAQEL
                     VPGRVGMISGLFFGFAFGMAGLGAAVLGELADLTDITFVYQLCSFLPAMGLLAAFLPN
                     LEPSGKVHATVVLEESAG"
     gene            complement(8352..8528)
                     /locus_tag="VFB89_01360"
     CDS             complement(8352..8528)
                     /locus_tag="VFB89_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HZM25978.1"
                     /translation="MLRWALLFLLIALVAAAFGFGGIATAAAGVAKVLFFIAIGIFAV
                     TLALGLVATRKIMR"
     gene            8671..>9042
                     /locus_tag="VFB89_01365"
     CDS             8671..>9042
                     /locus_tag="VFB89_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25979.1"
                     /translation="MTEPDHERPMTLGRVAVPPRHHLGSYREYEPPSHLALVAEAAWS
                     FRTTREISHRVLPDPAVSLAFWCCREPTGRPVSPRLALIGAKTRPHLVRFEAGCEITA
                     LRLKLEWIEPVLGLVPGDHDDA"
BASE COUNT         1541 a         2934 c         2893 g         1674 t
ORIGIN      
        1 cgatgaatac ggcaagcagc gcaatgggaa cgttcacaaa gaagatcgcg cgccacgaga
       61 acgcatcgac cagccagccg cccagcactg gaccaagcgc tgtcgtgagg gccgacgcgc
      121 cggcccaggt gccgatcgcc cgaccgcgct ccttctccgg aaaggtcgca ctgatgatcg
      181 ccaggctgct cggcaccaga atcgcccccc cgattccctg agcggcgcgc gcgacggtga
      241 gactgaaggc atcgggagcg aatccacatg cgaccgaggc tatcgtgaac gtggtgacgc
      301 ccaggatgaa catcatgcgc cgtccgaagc gatcgcccgc ggcgccgccc acgaggatga
      361 gcgcgccaag catcagcatg taggcgttga cgacccattg cgtctctctt accgaagcat
      421 tcaggtcggc ctggatggct ggaagcgcca cgccaaccac cgagccgtcg atgaacgcca
      481 tggccgagcc gaggatcgtc gtcgccagga cccatggctt ggccttctcc tcgcaatccg
      541 cgaccgctcg cggaccagcc tggatgactc cttcgtcgca gggggctcga ccgaacgtgg
      601 tcatactgct gagggggcca tagactgctc cgtaaggcgc aaggtatcgc ggccgagccg
      661 accaccgctg cgaccgggac tattccgaac gcgtcggacg atacaacatt ggtagtaatt
      721 ggatcgaggg ccgtacagtt ccgcaggcgg atgcgtgcag ctcaggaccc tgatcttctt
      781 cgtcaccagt cggtgcaacg ccaagtgtcg cacctgtttt tactggcagg agctgaaccg
      841 gccgggtgac ctcacctttg acgagatcgc taccctgacc cggaccatgc cccggttccg
      901 ggaactgtgg ctgtccggtg gtgagccgat gatgcgcccc cggctggccg acatcatcca
      961 gctcttttat cagcgcaatg agatccgcac gctcaatctg ccgtcgaacg gactgtttcg
     1021 tgaccggctg gtccggctca tggacttcat cacggcatcc ctgcccaagc tcaaggtcaa
     1081 cttgaacctg gcgctggacg gctttgccga gactcacgat cggatccggg gcgtgccggg
     1141 gaacttcgac caggcgatcc ggaccatcga agcgctctac cccgtgcggg accgaaaccc
     1201 caacatccga attcacgtaa actcggtaat cacggcagag aacatcgacg agctggagcc
     1261 cctgggtcga tggctgatcg agcatgcgcg tctcaacggc cagtactttc aggtggtgcg
     1321 tggtgaccca ctggatcccg ccctcaaatc ggtcggcccg gagcgtctgg ccgagttcta
     1381 tcgacgggtc gagccgctgc accggcatta cgggcggtat ctgtcgcagc gccacggggg
     1441 cgggccgaaa ggccgcctgg cggagttcta ctacaaccgc acactttcct tctactacga
     1501 ggttcagcac gctaacctgg agcagtccac ccgctggccc atgccgtgta cggcgggcca
     1561 gtcgattctg gtggtggact acaacgggga tgtccgcgcc tgcgagcttc ggcagaagat
     1621 tgcgaacctc cgcgaggtgg gctgcgactt tcgccgagtc cttcactccg aggcgctatc
     1681 ccgcgagacc gccggcatcg tgcgcgacca gtgctggtgc actcacgtct gcttcattca
     1741 cgacagcgcc aaatcgagcc gccgggcgca gctcttcgag attccattca agcgtcatca
     1801 ggtatagatg acgcttccgc tcgttctcct ctggggcgca gcggtctgct acaccctggt
     1861 cgtcaccgct atcagtgcgc ggctcgtcgg tgcggcgttg ccggggccac cctggatcgt
     1921 cgagcagatc gtgctccttc tgttctgggc ggcggccacg ccggcaattc tctggtcggc
     1981 tcggctcttc ccgctcgacc gccgagggcg ccgtctcggg caccttggcg tccacctgct
     2041 gtttgccttc gccttcattc tggccaccaa cctcgtcgcg ccggtgctga cccggcttat
     2101 cctgggccag tccttcgatg ttctggctgt gatgcggctg ggcctgctcg actttctgca
     2161 ggtgtatcac ctcgccctga tcgtgtacgc cttcattgtc ggcgtcggtc actatctccc
     2221 ggcccgggaa gcgcatcgag cccagcagct gcgcgcggag cgcctgcggg cgagtctggc
     2281 ggaagcgcgg ctccgcgcgc ttcaacttca gctcgaaccg cacatgctct tcaacgcgct
     2341 caacgccatc ggagctctgg tgatcacggg gaaaagggat gaggcgttcg atgccatcgg
     2401 gcgactgggc gacctcctcc gtgcggtgct cgcgaccgag tcccagcagg aagtgtcgtt
     2461 gcgcgaagag ctgcggctga cggagtcgta tctcgcgatc gaacgggccc ggctgggaga
     2521 ccggcttgat gcccgctggg agatcgcgcc tgggatcgag atggcccgga ttcccccact
     2581 cctgcttcaa ccgctggtag agaatgccgt acggcatggc atcgctcgta aagtcgaggg
     2641 gggccgcgtg gtggtaaggg cggatcgggt cggcgagcgt ttgcgcctgg aagtgcggga
     2701 tgatgggccg ggcgtgagcg aaggggccgc gtcggggagc ggcattggcc tcgagaacgc
     2761 tcgacgtcgg ctggagcaac tgtacggacc cgagcatcga ctcgatctcg agcggacgga
     2821 cgactggacc cgggtatcgc tcgagctgcc gtatcacggg atggacgagc ggagcgaggc
     2881 ggcatgacgt tgcgcgtagt gttggtggac gacgagccgc tggcccgggc ggcgctcgct
     2941 gctattctcg aggatcagcc cgatgtcgaa atcgccggcg agagcgcgtc tgcggcggac
     3001 gcggtacgcg tcgtccgcac tctggagccg gatctcgtgt ttctggacat cgccctgccg
     3061 gatcacgacg ggttctgggt gctggagcag ttggcgggca ggcagcctgc gattgtgttc
     3121 gtcacggctc attccgaccg ggccgtcaag gcctttgagg tccgtgccct ggactacgtc
     3181 accaagccgc ttcgtcgcca ccgggtcgaa gaggcggtgg cccgtgcccg cgagcgcacc
     3241 agcccgccgc cggacggacg ccgcgccgcc ggaggcccgc tcacgctcaa gacggatggc
     3301 actctccggc tcatcgatca ggacacgatc gactggatcg ccgccgacga cgatcacatc
     3361 gtggtccacg cgagcggcgg tcgctggcgc gcccgtgaaa ccttgcagga ggtcgagcgc
     3421 cgactcgatc cacgccgttt cgtgaggatc caccggtcga cggtcgtccg gctcaaccgg
     3481 gttcgcgagc tccagccctg gtttcacggt gactacgtcg cgattctgca caccggtgcc
     3541 aagctgcgag tgagccgaac ctatcgggaa cgagtcgccc ggtttctcgg gcgagacttg
     3601 tagcagtccg tttcaccgca ctttccgtcc ctctcaccgt actctcgatc gccccaccgc
     3661 aatgtcgctg acagtccggc gctttcagtc catcgtggag cgtctccgtc ccgggatccg
     3721 atatgcgccg catcatcggc ggactgctgc ttggatggct ggtattgtgc cgcgacgtcg
     3781 cggcccagaa cggctcgctc atgttccggg gcgatccggc tcacaccggt gtttccacgg
     3841 cagcgttctt ccagggccag ggcggtgtgc gctggcgagt gcgcaccggc gatgccgttc
     3901 ggtcatcacc cgcggtgacc cagacgcgcg tgtttgccgg gagcggtgat gggtacctgt
     3961 acgccataga ccgggccaat gggaaggtgg tctggcgcta cgcggccggc gggccagtcc
     4021 actcgtctcc ggccatcgcg ggcggactgg ttgtggccga gacgatggat gggcgcatct
     4081 tcgccgtcga ccagaagtcg ggcaagcagc gctggtcgat gaaaaccgga cccgcgctcc
     4141 cattcaacac cttccctgcc ggcgcctggg gctacatcgc gtcgtccccg gtggtggttg
     4201 gccaaagggt cgtcattgga gcaccggacg gcggcgtgta cgcgctcgac ctcacgagcg
     4261 ggaaacaacg ctggcacacg cggaccaacg gtcgggtgcg cgccacaccg gcggtggtcg
     4321 acggagtcgt ggtcgtcggc tcgttcgacg gccgggtgta cgccctcgac ctcgagaccg
     4381 gggtcgagcg ctgggtgcac aagaccgaag gcgacaccct ggattccgcc aagtggggct
     4441 tcgaccgacg tgccatccag ggctcggccg cagtctcaga cggaacggtg tacgtcggat
     4501 cccgcgacag cgggctgtac gcgatcgatt tcgcaaccgg acatcgccgc tggcgcttca
     4561 gccatcgtgg ctcgtgggtt ctcgcagcgc cgtcggtgcg ggacgggaag gtgttcgtag
     4621 gcagctccga cggccacttc tttcagggcg ttgattcgag cggcaaggaa ctctggcgcc
     4681 ttccgacgga cgccaacatg ctgggctcgc cgctgctcat aggaaacacg ttgctggccg
     4741 gcacctatcc tcgtgacacg cccaacgggg aacttctggc gatagacgcc ggcaccggca
     4801 acgttcgctg gaggctcaac ctcgacgcga gcgtgctctc gtcccctgtt gcattcgaca
     4861 atgagatcta cctcggtacg gaagacggga gcatcgtcgc agttggacag gcgagcccca
     4921 cgataccgcg gcttgccgtg ttctacgact cgacactcgg cggcaacgca tttgccaaag
     4981 gtggagcgct cgcctttgct tacttccgcg acctgggata ccagccgctt ggcgccgact
     5041 cgctcgcggc gttcctcaat gcgcgaatcg cggacagcgt gccgagcgcg gtcgtgttcg
     5101 ccaccgacgt gttgccgtcc aacgtggcgc cgacgcccag cgacaccacg ctgtttcggc
     5161 gctacctcaa tgccggaggg aaggtcgtct ggatcggcgg gccgatcgac gtattcgtcc
     5221 acgattcaac tggacagctg gtcgggttcg aggtaaaacg catggccgat ctgctgggaa
     5281 tctccgtcga cagcctggac ttcaacgaat acactgccca tcccaccgac gcaggccgac
     5341 gctggggaat cgatcgcgcg cttcgcggtg ccactcccat ggcccgttcc gcggtgagtg
     5401 aagcgcttgc cgtcgacgaa accggaatga ccacggcctg ggtccgccac taccggaccg
     5461 accggctctg gtccggcttc gttcagctct gggggctcgg cgccagctgg gatcggctcc
     5521 ccgcaatccg tgccgtcgcg gagtacggtt tgttgcgctg atctccttat cgtcacgatc
     5581 tgccaggtct cccctggcgg gactcgcctg ccggcggtat caatcccgga ctgcaccaca
     5641 ggggagcgat catggcgatt ggcgcgagtt ttctcgagca ggtgaaccgg agcttcgacc
     5701 gcgcggcgtc cttcacagga cacgacgcca ccctgctcgc caatatcaag gcctgcaaga
     5761 acctcttcta caccagcttt cccatcaagc tcgataacgg gcgcatcgag gtgatgcacg
     5821 cctggcgggc cgagcacagc catcacaagc tgccgtgcaa aggcggcatc agatacgccg
     5881 ccgatgtgga cgccgacgaa gttcaggccc tagccgcact gatgacgtac aagtgcgcgc
     5941 tggtagatgt gccgttcggg ggcgccaaag gtggtatccg gctcaacccc aagaaatact
     6001 cggaagggga actcgaacgc atcacgcgcc gcttcgtgtt cgagctcgta cgcaagaact
     6061 tcatcgggcc gggcctggac gtgccggccc ctgacgtcgg gaccagcccg cgggagatgg
     6121 cctggatcgt cgacacctac agccagctcc gctcgggtga gctggacgcg ctcggctgcg
     6181 tcaccgccaa gccgctgagc cagggtggca ttcgcggccg ggtagaggcc acaggtcgcg
     6241 gcgtgttcta cggcgtgcga gaggtcgtga gcatcgccga ggacatgaaa ccactaggcc
     6301 tctcccccgg gatcgcgggc aagcgggtcg tgattcaggg catgggtaac gtcgggtact
     6361 tcgctgccag gttcttccag agcggcggcg cggttgtggt gggcctggcc gagtccgagg
     6421 gcgccatctc gaatcccaag ggcctcgatg tggaccaggt catggtccac cgcaaggaat
     6481 gtggcggcat cctgggcttc cccggagcca ccaatctcgc ccgccgggag gatgcgctcg
     6541 agctggaatg cgatatcctc attcccgccg cgctcgaacg tcagatcacc gccgagaacg
     6601 cgcctaaagt caaggcgaag ctggtggtcg aagccgccaa tggaccgacg acttccgagg
     6661 ccgacgaaat cctcggccgg cgcggaatca cggtcattcc cgacgcctac atcaacgccg
     6721 gtggagtaac ggtctcctat ttcgaatggg tgaagaatct cgggcacgtg aggttcggtc
     6781 gaatgcagaa gcgcttcgag cagggcgcgt acgggcgatt gctcgaagcg gtcgagggca
     6841 tcaccggccg cagcttcacg cccgccgaaa tcgagcggct cactcatagt gcggacgagg
     6901 aggatctggt caactcgggg ctcgaagaga ccatgacgac ctcctaccat ccgatccgtg
     6961 aagtctggaa acagcatggc ggcaaggtcg atcttcggac tgcggcgttg atcgtcgcga
     7021 tcaacaaagt ggccgtgagc tacgaacagc tcggcatctt cccctagccg caagcgactg
     7081 gttggaaggg cccggagcgt ctatgctccg gcatgtcgaa cgatcagcag gtcccgcgca
     7141 ccgccttcgc ggttctcggc gcaatcagct tctgtcatct gttgaacgac atgatgcagt
     7201 cgctgttgcc tgcggtctat ccgatgctca aggccagctt cgccctcgat ttcgggcaga
     7261 tcggcctgat tactctcacc taccagctgg tcgcatcgct gctgcagccg gtgatcggac
     7321 actataccga ccagcgaccg caggcctatt cgctcgcggt cggcatggcc ttcacgctca
     7381 ttggacttct ggtcctcgcc cacgccgggg gcttcgcgac catcctcgcg gcggcggggc
     7441 tcgttggcgt agggtcgtcc gtcttccatc ctgaatcctc ccgtgttgcc cgcatagcct
     7501 cgggcggtcg gctcgggttt gcccaatccc tttttcaggt cgggggtaat gcgggatcat
     7561 cgctggggcc gctggtggcc gccttcgtcg tcgtgccgcg aggccaggcc agcatcggct
     7621 ggttttcggt cgccgcgctc ctcggcatca tcattttgtg gaacgtgggc cggtggtacg
     7681 gcgagcggca tggaccccgg gctaccaagg aatgggtggc tcaccagcct gcgacgtcga
     7741 cgctcccctc caagcaagtg cgaatcgccc tcgcggtgtt gatcgccctc atcttctcca
     7801 agtacatcta tctatcgagc ctgatcagct actacacctt ctacctgatc cacaaattcc
     7861 atctgtcggt ccagagcgcg cagatctacc tgttcgcgtt tctaggagcc gtggctgcgg
     7921 gcacgatcat cggaggcccg gtaggcgacc gggtcggccg caaatacgtc atctggtgct
     7981 cgattctcgg cgtgctgccg ttcacgctgg cgctgcctta cgccaacctc ttctggaccg
     8041 gcgtgctgag cgtgctgatc ggtctgatac tggcatccgc attttcggca atcctggtat
     8101 atgcccagga gctggtgccc gggcgggtcg gaatgatttc aggattgttc ttcggctttg
     8161 cgttcgggat ggccggattg ggtgcagcag tgctgggcga gctggccgac ctgaccgata
     8221 tcacctttgt ctatcagctt tgctcttttc ttccggcaat gggactgctc gccgctttcc
     8281 tgccgaacct ggagccgtcc ggaaaggtac acgcgaccgt cgtgttggaa gaaagcgccg
     8341 gctgagccga gctatcgcat gatcttcctg gtggcgacca ggccaagcgc caacgtcacc
     8401 gcgaagatcc cgatcgcgat gaagaagagc accttggcaa cgccagccgc ggcagttgca
     8461 atgccaccga atccaaacgc agcggcgacc aatgcgatca gcaggaaaag cagggcccac
     8521 ctgagcatct tcggctccaa ctttgagaaa ttgcggcgac gtgagtagcg cggcgccgac
     8581 tatcgacgcc agagtaccgc ttactggcga ttgcggtagt gaccaggctc acgaacagcc
     8641 gactgaatca ctgacacaac cccgctcgta gtgaccgaac ccgaccatga acgaccgatg
     8701 actctcgggc gggtcgccgt tccgccgcgc catcacctgg gtagctatcg cgaatacgag
     8761 ccgccgtcgc acttggcgct ggtcgcggaa gcagcctggt cgtttcggac cacacgggaa
     8821 atttctcatc gggtcctccc cgacccagcc gtcagtctcg ccttctggtg ctgccgcgag
     8881 ccgaccgggc gcccggtttc gcctcgtctg gcccttatcg gcgccaagac tcgcccgcac
     8941 ttggtgcgtt tcgaggccgg ctgcgagatc actgctttgc ggctcaagct ggagtggatc
     9001 gagcctgtcc tcggcctggt gccaggcgac cacgacgatg cc
//
LOCUS       DASRJQ010000057         2282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1096408, whole genome shotgun sequence.
ACCESSION   DASRJQ010000057 DASRJQ010000000
VERSION     DASRJQ010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096408"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="VFB89_01370"
     CDS             complement(<1..895)
                     /locus_tag="VFB89_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25980.1"
                     /translation="MVLPVLQEVAYVGLFWGIYALYRSVRLRDPWPLVAAGLAFVIGV
                     TIATPRIITIATEMSVVARTSTNVQNAAIEAVRYFGDGLLGRSQGEQRLLLIPPVNLH
                     EGVQLLSSSLAALAVIAIGLITPSRWLRFWSTTLLVVISVALIDFFPSFYKVGKVVLV
                     GGLIGGLVGGLLSNERVRGVVLERGIRRVSWGAIVFGLVVGLVGGLGLGLAGELLGEK
                     YPSLALQAFAANALLIGVPMWLLGRWLTRTSETGRVAGDRPDTVWREPPAVAEDLPFL
                     LGFVTLALAAILIPEVRLFLYY"
     gene            complement(895..1428)
                     /locus_tag="VFB89_01375"
     CDS             complement(895..1428)
                     /locus_tag="VFB89_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25981.1"
                     /translation="MFFVGYALFLFRLSLFEGWTYIGDSDRLNNMLNLRLFEVFSILK
                     RGSVPSWNEWEFMGYGVSGVHWMLPGAPPLPQLLAMVPLSELYRALGLLAVILFTATM
                     LATYWALGVYSIGPVQRVVGTLLYATGAYTFHKMTQLDIAFLALVAPPILHRLVYMTT
                     RERALGPFWGCHCAGQS"
     gene            complement(1806..>2282)
                     /locus_tag="VFB89_01380"
     CDS             complement(1806..>2282)
                     /locus_tag="VFB89_01380"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZM25982.1"
                     /translation="KANRAVDMRLYGSALEIPLPDGAVDAVLAYYAVHHMTGRTVAEN
                     RHKLELAFGELGRVVKPGGELLVFEVSPWRLVWHAERLFWNSVRGVLGDRLDMCFYPA
                     DVYEQVGRQTMPDARFSVQSFRTSLLSTFPPAFSLPWLPIPRFLYPFHVNLYRWRF"
BASE COUNT          504 a          719 c          715 g          344 t
ORIGIN      
        1 tgtagtacag gaagagcctg acctccggga tcaggatcgc cgccagtgcc agcgtaacga
       61 acccgagaag gaacggtaga tcctcggcaa cggccggcgg ctcgcgccac acggtatcag
      121 gacggtcgcc tgcgaccctc ccagtctcgg aggtacgtgt gagccatcga ccaaggagcc
      181 acattggcac cccaatgagc agggcgttag cggcaaaggc ctgcagcgcc agcgaagggt
      241 acttttcgcc gagcagctcg ccggccagcc ccagccccag cccgccgacc agtccaacga
      301 ccagaccaaa gacgatcgct ccccaagaga cccggcgaat tccccgttcg agaacgacgc
      361 ctctgactcg ctcgttgctt aggagtccgc cgacgaggcc gccgatgagc ccgccgacca
      421 ggaccacctt cccgaccttg tagaacgagg ggaagaagtc aatcaacgcg accgagatca
      481 cgacgagcag tgtggtactc cagaagcgca gccagcgtga cggggtgatc agcccgatcg
      541 cgatcactgc cagcgccgcg agcgacgagc tcagcagttg cacgccttcg tgcaggttga
      601 ccggaggaat cagcaggagc ctttgctcgc cctgagatcg cccgagcagg ccgtcaccga
      661 aataccgtac cgcttcgatg gcggcattct gaacgttagt cgatgtcctg gcaacgaccg
      721 acatctcggt cgcaatggta atgatgcgcg gtgttgcaat cgtcacccca atgacgaatg
      781 ccaggccggc cgccaccagg ggccagggat cgcgcaatcg gactgaccgg tagagggcat
      841 agatccccca gaacaggccc acgtaggcga cctcctgaag gacaggcaag accatcagga
      901 ctgaccagca cagtgacatc cccagaaagg tccaagagca cgttcgcggg tggtcatgta
      961 gacgagtcga tgcaagatgg gtggcgcgac tagcgcaagg aacgcaatat cgagctgggt
     1021 catcttgtga aacgtgtagg caccggtggc gtacaggagc gtgccaacga cgcgctgcac
     1081 cggcccgatg ctgtacaccc cgagcgccca gtaagtcgcc agcatggtgg ccgtgaacag
     1141 gatcaccgcc aggagtccga gcgcgcgata cagctcggag agcgggacca tcgccaacag
     1201 ttgaggcaac ggcggggcgc caggcagcat ccaatggacc ccagaaactc cgtaccccat
     1261 aaattcccac tcgttccagg acggaaccga gccgcgcttc aggatcgaga aaacctcgaa
     1321 cagacgcagg ttgagcatat tgttcaagcg atcggagtcg ccgatgtacg tccagccttc
     1381 gaacaggctc aaacggaaca ggaacagcgc gtagcccacg aaaaacaagc aggcgacgac
     1441 gtgggtaagg gccgggctga gcgcgatgcc ttgcaggacc gaggcgccca ggtctgcgac
     1501 atccgctgga gccgatcgct gtcgatcaac ggtcgtctcg gactcttgcg tcgcctgaat
     1561 gggtcgatcc ttgacgcgaa ttcgaggtca cgaacgctgc ggcgccgatt ctagcacatg
     1621 tccttcatca attccgatga gtcgctcagc ctcgaagtgc gacgtgtctt tcgcgttgag
     1681 aaggacagcg gttgtcgtgc ccgagtttgt cccgggagtt cgtgctgaag ggaacctgaa
     1741 agcccaggcg gcacgtcgac ttcacgcagc ataaacaccg tctgtgcttt ggagcccgag
     1801 cggatctaga accgccagcg atacaggttg acgtggaatg gatagaggaa gcgtgggatc
     1861 ggcagccacg gcaggctaaa cgccggcggg aacgtgctca gcagcgatgt gcggaacgac
     1921 tggaccgaga agcgagcgtc tggcatcgtc tgccgtccga cctgctcgta gacatccgcc
     1981 gggtagaagc acatgtcgag cctgtcgcca agcacacccc tgacggagtt ccagaacagg
     2041 cgctcggcgt gccagacgag ccgccatggg ctgacctcga agaccagcag ctcgccgccc
     2101 ggcttcacca cccgcccgag ctcgccgaac gcgagttcga gcttgtgccg gttctcggcc
     2161 acggtccggc ccgtcatgtg gtggaccgcg tagtacgcca ggacggcgtc cacggcgccg
     2221 tccggcagcg ggatctcgag cgcggaccca tagaggcgca tgtcgacggc ccggttcgcc
     2281 tt
//
LOCUS       DASRJQ010000058         2293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_4192517, whole genome shotgun sequence.
ACCESSION   DASRJQ010000058 DASRJQ010000000
VERSION     DASRJQ010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4192517"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..609
                     /gene="rfbC"
                     /locus_tag="VFB89_01385"
     CDS             55..609
                     /gene="rfbC"
                     /locus_tag="VFB89_01385"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HZM25983.1"
                     /translation="MRVEQTELPGVLLLTPRVFRDERGFFLESYNAREITAAGLPTNF
                     VQDNHSRSKAGVVRGFHYQLRHPQGKLIQVARGAIFDVVLDIRTGSPTFGKSVAVDLD
                     DEQHRMVWIPPGFAHAFCSLVDDTDVVYKCTDFYAPDDDRGVLWNDPDLSIPWPAIAR
                     TVSAKDASYQRLHAPRTDLPRYQP"
     gene            606..1481
                     /gene="rfbD"
                     /locus_tag="VFB89_01390"
     CDS             606..1481
                     /gene="rfbD"
                     /locus_tag="VFB89_01390"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HZM25984.1"
                     /translation="MTRLLVTGARGMVAREVLTQAKALGWQVTGRSHAELDITDSKAV
                     RTCVGATTPDLVLNAAAMAQVDVAEREPELARRVNAEAPGWLAEACASAGIPLVHLST
                     DYVFDGRARRPYRPQDGPNPLSVYGRTKWEGEEAVRQRLDRHLVVRTSWVYAANANNF
                     FTTIVQLAHQQETIRVVSDQHGSPTLAADLAHALLGAAAAAMTRSDLWGTYHFCNQGD
                     TTRYEFASAIVAGLAGRPGIVCHSVLPVPTSEHPRPAARPAYSVLDTNAWIAAFGQTP
                     RRWQEALSDALCQLN"
     gene            1466..>2293
                     /gene="rfbB"
                     /locus_tag="VFB89_01395"
     CDS             1466..>2293
                     /gene="rfbB"
                     /locus_tag="VFB89_01395"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: protein motif:HMM:TIGR01181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HZM25985.1"
                     /translation="MPAELSPSAHVPRRVLVTGGAGFIGSNFVRHLLASDASVRVVTV
                     DAVTYAGRLENLGSALKDPRHSFVEGDICDATMVRRIMSEHTVDTIVHFAAESHVDRS
                     ITGPAPFITTNVVGTFILLEAARDAWTEGRRTDVRFHHVSTDEVFGSLTESDPPFDDD
                     TPYAPNSPYSASKAGSDHLARAYHHTYGLPVTGTNCSNNFGPYQHPEKFIPTVIRSCV
                     NHQSVPVYGTGMNVRDWLYVDDHCRAVDQVVRCGRPGRIYLVGARNERQNLDVARLIC
                     "
BASE COUNT          405 a          731 c          735 g          422 t
ORIGIN      
        1 gcagacgggc tacggtcagt acctgcttca gatcgccggc gaggagcgtg gccggtgagg
       61 gtcgagcaga cggagttgcc gggggtcctt cttctcacgc cgcgagtatt tcgtgacgag
      121 cgggggttct tcctcgagtc gtacaacgca cgagagatca cggcggccgg gctgcctaca
      181 aacttcgtgc aggacaatca cagccggtcg aaggccgggg tggttcgcgg gtttcactac
      241 cagctcaggc acccgcaagg caagctcatt caagttgcgc gaggggcgat ttttgatgtc
      301 gtactggaca tccggacagg gtcgccgacg ttcggcaagt ctgtggccgt ggacctggac
      361 gatgagcagc accgaatggt ctggattccg cccggctttg ctcacgcatt ctgcagtctg
      421 gtggacgaca ccgatgtcgt ctacaagtgc accgactttt acgcgccgga cgacgaccgc
      481 ggggtgttgt ggaacgatcc ggacctttct attccctggc cggcaatcgc acggacggtc
      541 tccgccaaag acgcgagcta tcagcggctc cacgcgccgc gcaccgatct tccccgctat
      601 cagccgtgac tcgcctcctc gtaaccggag cacggggcat ggtggcgcgc gaggtgctga
      661 cccaggccaa agcgcttggc tggcaggtaa cgggacggag tcatgcggag ctggacatca
      721 cagactcgaa ggcagtccgc acctgcgtgg gcgcaaccac gccggatctg gtgctgaacg
      781 ccgcggccat ggcacaggtg gatgttgcgg agcgtgaacc cgagctcgcg cggcgggtga
      841 acgccgaggc acctggatgg ctggctgagg cctgcgcatc ggcgggcatt ccgctggttc
      901 acctctcgac tgactacgtg ttcgatggcc gggcccgccg cccataccgc ccacaagacg
      961 ggccaaaccc actgagcgtt tacggtcgca ccaagtggga aggggaggag gcggttcgtc
     1021 agcgcctgga ccggcatctc gttgtccgga cgtcatgggt gtacgcggcc aacgccaaca
     1081 atttctttac cacgatcgtg cagctggctc accagcagga aacgattcga gtcgtgtccg
     1141 atcaacacgg ctccccgact ctcgctgccg accttgccca tgcattgctc ggcgcagcgg
     1201 ctgccgcgat gacccggtca gacctctggg gtacctatca tttctgcaat cagggcgaca
     1261 cgactcggta tgagttcgcg tccgcaatcg tcgcaggtct ggccgggcgc cccggcatcg
     1321 tttgccacag tgtgcttccg gttcctacgt ccgagcatcc ccggcctgct gcacggccgg
     1381 cctattcggt cctcgacacc aatgcctgga tcgcggcgtt tggccagacg ccacggcggt
     1441 ggcaggaagc actatcggat gcgctatgcc agctgaattg agcccatccg ctcacgtgcc
     1501 ccgccgcgtg ctggtcaccg ggggggcggg cttcatcggc agcaatttcg tccgccacct
     1561 cctcgcgagc gatgcgagcg ttcgggtcgt cacggtcgac gcggttacgt acgccggccg
     1621 gctggagaac ctcggctccg cgttgaaaga tccgcggcac agctttgtcg aaggcgatat
     1681 ctgcgacgcg acgatggttc gccggatcat gagcgagcac acggtcgaca ccatcgtgca
     1741 tttcgctgcc gagtcacatg tagatcgctc gatcaccggg ccggcaccct tcatcaccac
     1801 caatgtggtc ggcaccttca tcctgctcga agcggcgcgc gatgcatgga ccgaagggcg
     1861 tcgcaccgac gtccgcttcc accacgtgtc caccgacgag gtgttcggga gtctgacgga
     1921 gagcgatccg ccattcgacg acgacacgcc gtacgcgccc aactcgcctt actcggcaag
     1981 caaggctggg tctgaccacc tggcgcgggc gtatcatcac acgtacgggc tgccggtgac
     2041 tgggaccaat tgctcgaaca acttcgggcc ttaccagcac ccggaaaaat tcattcccac
     2101 cgtgattcgc tcgtgcgtga accaccagtc ggtgccggtc tacgggactg ggatgaacgt
     2161 gagagactgg ttgtacgtgg acgaccactg tcgcgcggtc gatcaggtgg ttcggtgcgg
     2221 acggcctggt cggatctacc tggtgggggc gcgcaacgag cggcaaaatc tcgacgttgc
     2281 ccgcctcatc tgt
//
LOCUS       DASRJQ010000059         2262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_531616, whole genome shotgun sequence.
ACCESSION   DASRJQ010000059 DASRJQ010000000
VERSION     DASRJQ010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531616"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2262
                     /locus_tag="VFB89_01400"
     CDS             <1..>2262
                     /locus_tag="VFB89_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25986.1"
                     /translation="SVSLPSANRSRFASIQSGHPGVDPYTTAVSDAYQDLYGEGSFTG
                     KGIYDVDAFEQATHGRFPENTLLSHDLIEGNYARAGLATDILLYDDYPTRYLTFTRRK
                     HRWTRGDWQLLQWLAPTVPGPDGPEPNRLSLLSRWKILDNLRRSVVEIAQLVFLVAGW
                     TILPGSPIRWTLLGLGAIVAPWIASLLLAALRPPLDKSWRAYYGEVGRDAVTSLQQVA
                     LAITFLPHQAWVSADAIARTLWRLFVTHRNPLEWQTASQTERLVTGGPARVWRTMWPV
                     VALVVGIVLLVAATAWLRSGSTPVWPLGFAVLPLALLWSASPLIAYGLSAPAVRRERR
                     LTSSRRTAAMRYALLHWRFFDRFVTEASNWLAPDNYQDDPTPVVALRTSPTNIGLQLV
                     GTVSAYDLGFISGEELVRRLELAMRTLERLERFRGHFYNWYDLETLRILEPAYISTVD
                     SGNLAGHLIALRHACLSIVDAPVFDGRTWRAVETALTLANDRLRELSAPTPDSPVPKP
                     TTNAEEKLRVARLTLAAAPDPTALVDAVVPALVEARAAVAAAAPNGPGSEAYATEWID
                     WSLRLIEQHAPRIPGLTISKVMARGDRSLIRSESWRRLAERVPEAAELVQRLQALADR
                     AYRYALDMDFRFLYDETRALFSIGFQPRAHALDESYYDLLASEARLASFIAVAKGDVP
                     VEHWFRLGRTLTHAAGETALVSWSGSMFEYLMPALVMRSFPFTVLERTQYAAVRRHIA
                     YGAERGVPWGASES"
BASE COUNT          336 a          773 c          736 g          417 t
ORIGIN      
        1 agcgtgtcgc tgccgagtgc gaaccgctcc cgctttgcgt cgattcaatc gggacatccc
       61 ggggtcgacc cctacacaac cgcggtatcg gacgcctacc aggacttgta tggcgagggc
      121 agcttcaccg gcaagggcat ctacgacgtg gatgcgttcg aacaggcgac gcacggccgc
      181 tttcccgaga acacgctgct gtcgcacgac ctgatcgaag gcaactacgc ccgcgccggg
      241 ctcgcgacag acatcctgct ctacgacgac tatccgaccc gctacctcac gttcactcgg
      301 cggaagcatc gctggacccg gggcgactgg cagctgctgc aatggctggc ccccacagtg
      361 cccggtcccg acgggccgga gcccaatcgc ctttcgcttc tgtcccgctg gaagattctg
      421 gacaacctgc ggcgaagcgt ggtggagatc gcgcagctcg tgttcctggt ggcgggctgg
      481 accattctgc ccgggtctcc cattcgctgg accctgcttg ggctcggcgc gattgtagcg
      541 ccgtggatag cctcgctgct cctggccgcg ctcaggcctc ccttggacaa gtcatggcgg
      601 gcctattacg gggaagtggg ccgcgacgcg gtgaccagtc tccagcaggt agcgctcgcc
      661 atcacgttcc tgccacatca ggcatgggtt tccgccgatg ccatagcgcg cacgttatgg
      721 cgactgttcg tgacccaccg gaacccgctc gagtggcaga ccgcatctca gaccgagcgg
      781 ctggttaccg gagggcccgc cagggtctgg cgcaccatgt ggcctgtggt cgcgttggtg
      841 gtcggcatcg tgctgctggt cgccgcgact gcctggcttc ggagcggcag cacaccggtc
      901 tggccgctgg gctttgccgt gctgcctctc gccctcctct ggtcggcatc gccgttgatc
      961 gcgtacggac tcagtgcgcc ggcggttcgg cgcgagcgac gattgacctc atcccgccgg
     1021 accgccgcga tgcgctacgc gctgctgcat tggcgattct tcgaccgctt cgtcaccgaa
     1081 gcgtcgaact ggctcgcgcc ggataactat caggacgatc ccacgccggt cgtcgcgctc
     1141 cgcacctcgc ccaccaacat cggacttcag ctcgtcggca cggtgagcgc ctacgatctc
     1201 ggcttcatct cgggcgagga gctggtccgg cggctcgagc tggcaatgcg cacgctggag
     1261 cggctcgagc ggttccgcgg acacttctac aactggtacg atctcgagac cctgcggatt
     1321 ctcgagcccg cgtacatctc gactgtggac agcggcaacc tcgccggtca tctgattgcg
     1381 ctgcggcacg cctgcctttc catcgtggat gcgccggtgt tcgatgggcg cacctggcgc
     1441 gccgtggaga cggcactgac cttggcgaac gaccggcttc gcgagctgag cgctcctacc
     1501 cccgattcgc ccgtgccaaa acccaccacc aatgccgagg agaaacttcg cgtcgcacga
     1561 ctgacgctcg ccgccgctcc tgacccgact gctctggtcg acgccgttgt cccggctctg
     1621 gtggaggctc gcgctgcggt cgccgccgcg gcacccaacg gcccggggtc cgaggcatac
     1681 gcgacggaat ggatcgactg gagccttcgg ttgatcgagc agcacgcgcc ccggattccg
     1741 ggcctgacga tctcgaaagt gatggcccgc ggagatcgca gtctgattcg ctccgagagc
     1801 tggcgcagac tcgcggagcg cgtgccggaa gcggcggagc tggttcagcg gctgcaggcg
     1861 ctcgccgatc gggcgtaccg gtacgcgctc gacatggatt tccgttttct ctacgacgag
     1921 acccgagcct tgttctcgat cggcttccag cctcgggcgc atgcgctgga tgagtcgtac
     1981 tacgacctgc tcgcgtccga ggcacggctg gccagcttca ttgccgttgc caagggcgac
     2041 gtgccggtcg agcactggtt ccggctcggg cgaacgctga cccacgccgc cggcgagacc
     2101 gctctcgtgt cgtggagcgg cagcatgttc gagtacctga tgcccgcgct cgtcatgcgg
     2161 tcgtttccct tcaccgtcct ggagcggacc cagtatgcgg ctgttcggcg tcacatcgcc
     2221 tatggggctg agcggggcgt gccgtggggt gcgagcgaaa gc
//
LOCUS       DASRJQ010000060         3843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2763842, whole genome shotgun sequence.
ACCESSION   DASRJQ010000060 DASRJQ010000000
VERSION     DASRJQ010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2763842"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VFB89_01405"
     CDS             complement(<1..660)
                     /locus_tag="VFB89_01405"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZM25987.1"
                     /translation="MSRLKVGVIGAGAWGKNHVRTLASLPETSLAAVCEVDPGIRDRL
                     TKQYPGTLVTDSVSALLERVEAVVIASSARTHAELGRKAVEAGIPALVEKPFALTVAD
                     AEGLADLAARRGVPVLAGHLLEYHPVVEQLRRLVSERALGDLYYLYSQRVNLGQVRPD
                     ENALWSFGPHDVSVALFLLGDLPCTVAAQGHSYLQPGIEDVVFLTLTFASGVVAHAQM
                     SW"
     gene            complement(657..1772)
                     /locus_tag="VFB89_01410"
     CDS             complement(657..1772)
                     /locus_tag="VFB89_01410"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZM25988.1"
                     /translation="MTVPLLDLVAQYQSIKDAVMPALQSVIERQQFIMGPEIGQLEAE
                     VARLSHTKHGIACASGTDALLLPLKSLRLEPGDEVIAPAFTFFATAGTIHNAGGTPVF
                     VDIDPSTFNLDPAAVEAAITPRTRAIVAVHLYGQMAAMERLLPLALRHKLFLIEDAAQ
                     AIGARRQMDGEWRVAGELGWASALSFFPSKNLGAWGDGGMMLTQDDALAERLRKLRLH
                     GGAKQYHHEEVGTNSRLDTLQAAVLLVKLGYLAGWSAARRDRAARYSHAFAGSADICP
                     PTVDPANEHIFHQYTIRVPRRDDLAAHLKSKGIGHAIYYPTGLHLQPCFGHLGYRRGS
                     LPATEDAMASVLSLPIYPELTVAQQNIVIEAVRDFYR"
     gene            complement(1817..2407)
                     /locus_tag="VFB89_01415"
     CDS             complement(1817..2407)
                     /locus_tag="VFB89_01415"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZM25989.1"
                     /translation="MTDYFVHSSSFVDSGAVIGSGTKIWHFSHVMPGAVIGTGCNLGQ
                     NVVVMPGTRIGNNVKIQNNVSIYEGVTLEDDVFCGPSCVFTNVLNPRSHVSRKREYQP
                     TLVRRGASIGANATVVCGVTLGEYCFIGAGAVVTTDVPAFGLMLGVPARRVGWMCQCG
                     VRLKVVNGSAKCPTCGAGYREENALLTLIDPPGKGG"
     gene            complement(2466..3449)
                     /gene="murB"
                     /locus_tag="VFB89_01420"
     CDS             complement(2466..3449)
                     /gene="murB"
                     /locus_tag="VFB89_01420"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HZM25990.1"
                     /translation="MDRVTARRPAFVAALKERVRGEVRESEGLARYSTYRIGGPATVL
                     LPARAEDIAAAVQLATGEGVGCFVLGLGSNLLLPDEGLDALVIRLGKGLDELVRDGNR
                     WRIGAGLPAPLAARRTGEAGQAGLHKFVGVPGTVGGGVYMNAGCHGGDWSEVVEQVTV
                     IDADGDLRVLERREIPFSYRRSGLDGLIVVGTTVALRPENPATLSAELSELFRWRQEG
                     TPFNQPCCGSVFQNPGGPSWKRKEGPRTAGQLIEAAGLKGLRVGAAEVSPMHANYFVN
                     TGGATAADVRGLIARVQQAVEEKFGVRLETEVKLIGSRGNYLNINSVNGER"
     gene            complement(3437..>3843)
                     /gene="carB"
                     /locus_tag="VFB89_01425"
     CDS             complement(3437..>3843)
                     /gene="carB"
                     /locus_tag="VFB89_01425"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF014225.4"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HZM25991.1"
                     /translation="NDNDKNNVVPIVRRFHALGFRILATAGTVQHLRARGIPAERVLK
                     VYEGRPNAIDLIVSGAVQLLINTPLGKLTQQDDYAIRRAALQHRLPYTTTLSAASAAC
                     DAIIALRSRAGEVRSLQEWHALAREMSAEWTG"
BASE COUNT          706 a         1306 c         1180 g          651 t
ORIGIN      
        1 ccacgacatc tgcgcgtgcg ccaccacgcc ggacgcgaat gtcagcgtga ggaagacgac
       61 gtcttcgatg cccggctgga gatacgaatg cccttgcgcc gccaccgtgc agggcaggtc
      121 cccgagcaaa aagagcgcca ccgacacatc gtgcggcccg aagctccaga gcgcattttc
      181 gtcgggacgg acctgcccaa gattgacccg ctgcgagtac aagtagtaca gatccccgag
      241 agcacgctcg ctgaccagcc gccgcaactg ctccaccaca gggtggtact ccagtaagtg
      301 cccagccaga accggaacac cgcggcgtgc cgcgaggtcg gcgaggcctt cggcatctgc
      361 caccgtcaga gcaaacggct tttcgaccag cgcagggatg cccgcctcga ccgccttccg
      421 ccccagctcg gcatgggttc gcgcgctcga ggcgatcaca accgcttcga cccgctcgag
      481 aagagccgat accgagtcgg ttaccagcgt tccgggatac tgcttggtga gacggtctcg
      541 gatgccgggg tccacctcgc acacagccgc caggctggtt tccggcaggg acgccagcgt
      601 gcgcacgtgg ttctttcccc aagccccggc tccgattacg ccgactttga gccggctcat
      661 cgataaaagt cccgcaccgc ctcgatcacg atgttctgct gggcgaccgt cagctcggga
      721 taaatcggaa gcgacaggac gctcgccatc gcatcttccg tggccggcaa gctgccccgg
      781 cgatagccca gatgaccaaa acagggctgc aggtgcaggc cggtcggata gtagatcgcg
      841 tgaccgatcc ccttgctctt gagatgggcc gccaggtcat cacgccgggg tacccgaatg
      901 gtgtactggt ggaagatgtg ctcgttggcg ggatccaccg tgggcgggca gatatctgcg
      961 ctgccggcga acgcgtggga gtaccgcgcc gctcggtctc gccgggccgc actccagccg
     1021 gcgagatagc ccagcttcac gagcagcaca gcagcctgca acgtgtccag cctgctgtta
     1081 gtgcccacct cctcgtggtg gtactgcttg gcccctccat gaaggcgaag cttccgtagc
     1141 cgttccgcca gcgcgtcgtc ctgcgtcagc atcatcccgc cgtcgcccca cgcaccaaga
     1201 ttcttgctcg gaaagaacga gagagcgctg gcccaaccca actccccggc aacccgccac
     1261 tcgccatcca tctgccgtcg ggcaccgatg gcctgggccg cgtcttcgat caggaaaagc
     1321 ttgtgccgca gcgctagcgg cagcagccgc tccatggccg ccatctggcc gtacaggtga
     1381 acggcaacaa tggcgcgcgt gcggggggta atcgctgctt cgaccgcggc gggatcgagg
     1441 ttgaaggtcg atggatcgat gtccacgaag accggcgtac cgccggcgtt atggatagtc
     1501 ccagccgtgg cgaagaacgt gaacgccggg gcaatgactt cgtcgccggg ctcgagccga
     1561 agcgacttga gcgggagcag cagggcgtcc gtaccgctgg cgcaggcgat gccgtgcttc
     1621 gtgtgggaga gccgagccac ctcggcttcc agttgcccga tctccgggcc catgatgaat
     1681 tgctgccgct cgatgaccga ctggagggcc ggcatcacgg cgtctttgat cgactggtat
     1741 tgggcaacga gatccagcag cgggacggtc aatgctcacc tgctcagaat ggtgaaaccc
     1801 atgaaagcca aagaacttag ccccccttcc ccggtgggtc aatcagcgtg aggagggcgt
     1861 tctcttcgcg ataacccgcc ccgcaggtcg gacacttcgc gcttccgttc acgaccttca
     1921 agcgcacgcc gcattggcac atccacccta cccgacgggc ggggacccct agcatcaaac
     1981 caaaggccgg gacgtcagtc gtgaccactg caccagcccc gatgaagcag tactccccga
     2041 gggtgacccc gcagacgact gtcgcgttgg ctccgatcga cgctcctcgc cgcaccaggg
     2101 tcggctgata ctcgcgcttc cgggacacgt ggcttcgcgg attgagcacg ttggtaaaca
     2161 cgcagctggg tccgcagaag acgtcgtctt ccagggtgac gccttcgtaa atcgagacgt
     2221 tgttttggat cttcacgttg ttgccgatgc gagtcccggg catcaccacg acgttctgac
     2281 ccaggttaca ccccgtccca atgacggcac ccggcatgac gtgactgaag tgccagattt
     2341 tggtcccgga cccgatgacg gcgccggaat caacgaagga agatgagtgg acgaagtagt
     2401 cggtcatagg aaaagcgtga acggttaacg ggggttcccg gtgataggcg cctcccgttt
     2461 accgtttacc gttcaccgtt cacgctgttg atgttgagat agttccctct cgacccaatc
     2521 agcttgactt ccgtctccag ccgcactccg aacttctcct ccactgcctg ctgaacccga
     2581 gcgatcaagc ctctcacgtc cgccgcggtg gcgccgccgg tgttcacaaa gtagttcgcg
     2641 tgcatgggcg acacctccgc tgccccgacg cgcaagccct tcaacccggc agcctcgatc
     2701 aactgccccg cggtgcgtgg accttcctta cgcttccagc tggggccgcc aggattctga
     2761 aagacgctgc cgcagcacgg ctgattgaaa ggcgttccct cttgccgcca gcggaacagc
     2821 tccgataact cggcgctgag ggtagccgga ttttccggcc gcagcgccac cgtcgtaccg
     2881 accacgatca gtccgtcgag cccgctgcgg cgataggaga agggaatttc gcggcgctcc
     2941 aatacccgaa ggtctccatc ggcgtcgatc accgtgacct gctcgacgac ttcggaccag
     3001 tcgcccccgt gacagccggc gttcatgtaa accccgcccc ctaccgtgcc cgggacgccc
     3061 acgaacttgt ggagacctgc ctgacccgcc tcgcccgtac gccgcgcagc cagaggtgcc
     3121 ggcagcccgg cgccgatccg ccagcggttt ccgtcccgca ccagctcatc gagccctttg
     3181 ccgagccgga tgaccaacgc atcgaggcct tcgtccggga gcagcaggtt ggagccgaga
     3241 ccgagaacaa agcagccgac cccctcgccg gtcgccagct gaaccgctgc cgcgatgtcc
     3301 tcggcacggg ccggaagcag gacagtggcc gggccgccga tgcgataggt ggagtagcgt
     3361 gccagccctt ccgactcccg aacctcgccc cgcacgcgct ccttcagcgc ggcgacgaac
     3421 gccggccggc gagccgtcac cctgtccatt cagccgacat ctcgcgggcc agggcgtgcc
     3481 attcctgaag cgaccgaacc tcgccggcgc ggctccggag cgcgatgata gcatcgcagg
     3541 ccgcggatgc cgccgacagc gtggtcgtgt acggcagccg atgctgcagt gcggcgcggc
     3601 ggatggcgta gtcgtcctgt tgggtgagct tgccaagcgg agtgttgatg agcagctgaa
     3661 ccgcgccgga gacaatcagg tcgatggcgt tggggcgtcc ttcgtacacc ttgagcaccc
     3721 gctccgcggg aatgccgcgt gcacgcagat gctgaaccgt acctgctgtg gccaagattc
     3781 tgaacccaag cgcgtggaag cgccggacga tcggcaccac attgttcttg tcgttgtcgt
     3841 tca
//
LOCUS       DASRJQ010000061         4538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2763041, whole genome shotgun sequence.
ACCESSION   DASRJQ010000061 DASRJQ010000000
VERSION     DASRJQ010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4538
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2763041"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VFB89_01430"
     CDS             <1..768
                     /locus_tag="VFB89_01430"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HZM25992.1"
                     /translation="TASKDGAPTATVRRLEGAQAAGTGEGGQVIVEGAPVSMGREAKV
                     TEAGNYRFFAGWRSDPFFFDAGALDNFKWTGTDFFGDKDICSIALEASNAVLGSSEVR
                     LWARTVVRDSGRWVQVDRGARASQTPFLTGDQNEAYRAAEPKDDARFIAAFAHSLEHI
                     GGLAPEDAKQVAATLLPDLLRYDPTRPASYPQNGRKPSDDAADAFLTVITNGKLTGDG
                     IGPHADLLAEFPYLGPPHSDRSLEAQTARHQTLVESR"
     gene            826..1686
                     /locus_tag="VFB89_01435"
     CDS             826..1686
                     /locus_tag="VFB89_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZM25993.1"
                     /translation="MPTIKTTDGTEIYYKDWGTGQPIVFSHGWPLSADDWDTAMLFFL
                     QQGYRVIAHDRRGHGRSTQTSDGHDMDHYAADLAALTAHLDLKDAVHIGHSTGGGEVV
                     HYIARHGESRVAKAVIASAVPPLMVKTPANPGGLPKEVFDGLQAQLAANRSQFYIDLP
                     SGPFYGYNRPGAKPSQAIIWNWWRQGMMGGAKAHYDGIVAFSQTDFTEDLKKITVPVL
                     VMHGDDDQIVPYADSAPLSAKLLRNGTLKTYKGFPHGMPTTEADTINRDILAFIKQPA
                     ASPRREPALV"
     gene            1865..2329
                     /locus_tag="VFB89_01440"
     CDS             1865..2329
                     /locus_tag="VFB89_01440"
                     /inference="COORDINATES: protein motif:HMM:NF028004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HZM25994.1"
                     /translation="MKTKRLQRIAIVAVLVSGVGGSTISAQNKSTQDKYTLKVPGGLA
                     FSEFKGFEGWHLVSVSQDGGLFAAILANPVMIDAYQKGIPQNGKPFPDGSKMAKIHWN
                     PKKLETFPAATVPGTQHDVDFMVKDSKRFKDSGGWGWGAFEARLEVPRAGTA"
     gene            2361..3209
                     /locus_tag="VFB89_01445"
     CDS             2361..3209
                     /locus_tag="VFB89_01445"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZM25995.1"
                     /translation="MTTTLSQSIQPQVRTIDGLSIRYAESEPRDDHALLLSPWPESIY
                     CYEPTWTRLAEHTHLVAIDLPGFGQSERRNSLMSPRAMGEFIVRVADAFGLKQPHVVG
                     PDVGTAASLFAAALYPSRFRSLVVGTGGTAVPLQLGDPLKEWVEAPSLEPYRKIDGRQ
                     VITFAIGTLERYRPTEAAREDYLSSYAGERFAESMRYVQTYPQELPVLRDLLPTIETP
                     VQILAGARDSVVPPVNAEFLHERLPKSKLEFIDAGHFIWEDAADQYAALVTSWWAGGY
                     AAEERS"
     gene            3364..3489
                     /locus_tag="VFB89_01450"
     CDS             3364..3489
                     /locus_tag="VFB89_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25996.1"
                     /translation="MPKRAARCSDFVIRYYLADHKRRIAVARVDGRLEGSVHIRV"
     gene            3513..4226
                     /locus_tag="VFB89_01455"
     CDS             3513..4226
                     /locus_tag="VFB89_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM25997.1"
                     /translation="MTASTAAVPSSTSVSTTPAFWERLWRTSGLQFVVLFILASLIYG
                     SQPQVGASADALAAFYTGDRTRILIAAVFSGLNLLNLLWFAAALRTTLADAGQEGWGA
                     AATASSAAFGALFFLQITVSGVLAYSIAGSGNLLLTSGLNDFVWGLGVLSSFPRAMLI
                     MSGVFGLWRAGFISNALFTAGVGGVVLGVLGGTTWLHGFWAPDGAYSRFVSPLILLLW
                     VVVVSRVLLARGPSARGAW"
BASE COUNT          803 a         1470 c         1461 g          804 t
ORIGIN      
        1 acggcttcca aggacggcgc gccgacggcg acggtgcgcc gtctggaggg cgcgcaggcc
       61 gccgggacgg gcgagggcgg gcaggtgatc gtcgagggag cgccggtctc gatgggtcgg
      121 gaagcaaagg tgacggaggc cgggaactat cgcttttttg ctggctggcg cagcgatccc
      181 ttcttcttcg acgccggtgc cctcgacaac ttcaaatgga cgggcacaga tttcttcggc
      241 gataaagaca tatgcagcat cgcgctggag gcgtccaacg cggtgctggg gtcgagcgag
      301 gttcggctgt gggcccgcac ggtggtccgg gacagcggac gttgggtgca ggtggaccgg
      361 ggtgcgcgag cctcacaaac tcctttcctt accggcgatc agaacgaggc ttatcgcgcc
      421 gcggagccga aggacgatgc ccgtttcatc gctgccttcg cacactcgct ggagcatatc
      481 ggcggcttag cgccggagga cgccaagcag gtagcagcca ccctgttgcc ggacctcttg
      541 cgctacgacc ccacccgtcc ggcgtcctac ccgcagaatg gccgcaaacc cagcgacgac
      601 gcggccgatg ccttcctgac tgtcatcacc aatgggaagc tgaccgggga cgggataggc
      661 ccgcacgccg atctgctcgc cgagtttccc tacctggggc cccctcacag cgaccgatca
      721 ctcgaggctc agacggctcg tcaccaaacg ctagtggaga gtcgctaacc gtcgagccga
      781 cgcgaggaag ctgcccacgg ctcgttaact catacggagg ctactatgcc aacgatcaag
      841 accaccgatg gaaccgagat ctactataag gattggggta cgggacagcc gatcgtgttc
      901 agccacgggt ggccactgtc ggctgatgat tgggacacgg cgatgctgtt tttcctgcag
      961 cagggctatc gagtcatcgc gcacgaccgg cgcggccatg gccgctccac ccagacgagc
     1021 gatggccatg acatggatca ttacgccgcc gacctggcgg cgctgaccgc acatctcgac
     1081 ctcaaggacg cggtgcatat cggccactcg accggcggcg gtgaggttgt gcactacatc
     1141 gcgcgacacg gcgagagccg ggtggcgaag gcggtgatcg cgagcgcggt gcccccgctc
     1201 atggtaaaga cgccggcgaa tccgggcggg ctccccaagg aggtgttcga cggattgcaa
     1261 gcgcagctcg ccgccaatcg atcccagttc tacatcgatc tgccgtccgg gccgttctat
     1321 gggtacaacc ggccgggggc gaagccctcc caggcgatta tctggaactg gtggcgccag
     1381 ggcatgatgg gaggtgccaa ggcgcactat gacggcatcg tcgccttctc tcaaacggac
     1441 ttcactgaag atctgaagaa gatcaccgtg cccgttctgg tgatgcatgg cgatgacgac
     1501 cagatcgtcc cgtatgccga ttcggcgccg ctgtcggcaa agctgctcag gaatggcacc
     1561 ctgaagacct acaaaggctt tccgcacggc atgcccacga ccgaggctga caccatcaac
     1621 cgcgatatcc tcgcattcat caaacagccg gcggccagcc cgagacgcga gccggctctg
     1681 gtctgacctt gacccgaagt ccttctcgcg tgctggatgg tgatggcggt cccagcccca
     1741 cctcagcgca tctggacgcg acgtcgtcca ggtgcgcgag gacttcgacc ctggctatgt
     1801 ctcccgaggt atacctatcg aggcgacgag atcatctacg tcctcgacac aggaggaact
     1861 ggtgatgaag accaagcgcc tgcagaggat tgctatcgtg gcggtactgg tcagtggcgt
     1921 gggcggcagc accatatccg cccagaacaa gtcgacccag gacaagtaca ccctgaaggt
     1981 gccgggtggg ctcgcgttct ccgagttcaa gggattcgag ggctggcatc tggtctccgt
     2041 cagccaggac ggaggcctgt ttgccgcgat cctcgccaat cccgtgatga tcgacgccta
     2101 ccagaagggg atcccccaaa acggcaagcc gttccccgac gggtccaaga tggccaagat
     2161 ccattggaac ccgaaaaagc tggagacgtt tcccgccgcg acggtgccgg ggacgcagca
     2221 tgacgtcgac ttcatggtga aggacagcaa gcggttcaag gacagcggcg ggtggggatg
     2281 gggcgcgttc gaggcacgcc ttgaggtgcc gcgagctggc accgcctgag ggtgaattgc
     2341 caaccgtgag gaaggaaccc atgaccacaa cgttgagcca atcgatccaa ccgcaggtcc
     2401 gcaccatcga cggtctgtcc attcggtatg ccgagagtga gccgcgagac gaccacgccc
     2461 ttttgctgag cccgtggccg gagagcattt actgctacga gcccacctgg acccggctgg
     2521 ccgagcacac ccacctagta gcaatcgatc tgccggggtt cgggcagtcc gagcgccgca
     2581 attcattgat gtcgccacgg gcgatgggcg agttcatcgt tcgagtcgct gacgctttcg
     2641 gcctgaagca gccgcacgtc gtggggcccg acgtcggaac cgcagcgtcg ctcttcgccg
     2701 cggcactata tccgagccgg ttccggagcc tcgtcgtcgg caccggcggt acggcggttc
     2761 cgctccagct cggtgacccg ctgaaggaat gggtggaggc cccgtcgctc gaaccctatc
     2821 gcaagatcga tggacgccag gtcatcacct tcgcgatcgg aaccctcgag cggtaccgcc
     2881 ccacggaagc cgctcgcgag gactatctct cgtcctacgc aggcgagcgg ttcgccgagt
     2941 cgatgcgcta cgtgcaaacc tacccgcagg agcttccggt attgcgcgac ctgctaccga
     3001 cgatcgagac gccggtgcag atcctcgccg gcgcgagaga cagcgtggtg ccgccggtca
     3061 acgccgagtt tctccacgag cggctgccca agagcaagct cgagttcatc gacgccggcc
     3121 acttcatctg ggaggatgcc gccgaccagt acgcggcctt ggtcacgagc tggtgggctg
     3181 gaggctacgc ggcagaggaa cgctcgtagc cgcttcggga atagtgccga tggcagcgga
     3241 ctggggactc ccgactgcca ctcaacgatc gattgccccc gctccaatct gggatctcac
     3301 ccaggggcaa tcattaagca aggaaacggc ctttcggcgc tcacgtgttc ctgcgcgtcc
     3361 acgatgccca agcgggccgc acgttgctct gacttcgtca ttcgctacta cctggccgac
     3421 cacaagcgac ggatcgcggt cgcgcgtgtc gacggccggc tcgaaggcag cgttcacatc
     3481 cgagtctgac ctgattcgag ggagggggtt ccatgaccgc atccaccgct gccgtgccct
     3541 catccaccag cgtgtccacc actcccgcgt tctgggaacg cttgtggcgc acgagcggcc
     3601 tccagttcgt cgtcctgttc atcctcgcgt cccttatcta cggctctcag ccccaggtcg
     3661 gtgcctcggc cgacgctctc gccgcgttct acacgggcga tcgcacacgg atcctgatcg
     3721 cggcggtgtt ttccggcctc aacctcctca acctcctgtg gttcgccgcg gcgctcagga
     3781 ccaccctggc ggatgcggga caagaggggt ggggcgcagc ggcgaccgca tccagcgcgg
     3841 cgttcggagc gctgttcttc ctgcagatta ctgtgagcgg agttctcgcc tactcgatcg
     3901 ccggctccgg caatctcctg ctcacgtccg gactgaacga cttcgtgtgg ggcctcggcg
     3961 tattgagctc gttcccgcgc gcgatgctga tcatgtccgg ggtgttcggg ctctggcggg
     4021 ccgggttcat ttcgaatgcg ctctttacgg cgggggtcgg cggcgtcgtg ctcggcgtgc
     4081 tgggcggcac gacgtggctg cacggattct gggcaccgga cggcgcgtat tcgcggttcg
     4141 tctcgccact gatcctcctc ctctgggtcg tggtcgtaag ccgggtcctg ctggcccgag
     4201 ggccctctgc acgtggtgcg tggtgacgcc gagagacgac cgcggccagc ttcggattcc
     4261 ccgaagcgat ctggcgttga gaccggtctg gcttatcctc acgttggctc tggggccgtc
     4321 gtccctccaa ggagggcgct gtcggctcag caggctcgcc gctcccgctc ggctgacagg
     4381 cctccgcgca tgccctcatc agacaccagg agttgccaaa cttctggctt cctccacatg
     4441 cttgctagcg tcctctcggt cagcgggggc aaagccgagc aatgaacgga aatacggcgc
     4501 aagcggtggc cacgcgaccg tcgcccgagt ctgcgctg
//
LOCUS       DASRJQ010000062         6901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_240555, whole genome shotgun sequence.
ACCESSION   DASRJQ010000062 DASRJQ010000000
VERSION     DASRJQ010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6901
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240555"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..2090)
                     /gene="glgX"
                     /locus_tag="VFB89_01460"
     CDS             complement(18..2090)
                     /gene="glgX"
                     /locus_tag="VFB89_01460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HZM25998.1"
                     /translation="MIRVRPGSPAVLGAHWDGEGTNFALSSEHATAVEVCLFEGPDDT
                     REALRVPVRERTGIVWHTYLPDAQPGQLYGYRVHGPWSPQTGHRFNPAKLLLDPYARA
                     ITGSVRWTDALSGYVVGHPEQDLIPDPRDSAGGLPKCMVVESAFTWADDRPPRTPWAD
                     TVIYECHVKGMTMRHPEVPAPLRGTYLGLAADAVINHLKSLDVTAVELLPICHFVTER
                     TISDRGLVNYWGYNTIGYFAPDVRYASGRLGQQVTEFKTMVKRFHRFGIEVILDMVFN
                     HTAEGNHLGPTLCLRGVDNAGYYRLDPTNPRHYLDFTGCGDTLDIRRPQALRLVLDSL
                     RYWVQDMHVDGFRFDLGPILGRDPGDEFNAQARFFEVVRQDPVLADVKLIAEPWDLGP
                     NGYQLGAFPAGWADWNGKYRDAVRGFWRGDPGLLPELATRLAGSADLFGPADRTPQAS
                     VNFVTCHDGFTLNDLVSYERKHNEANGEANQDGNDHNLSRNWGVEGPTDAPHIARVRE
                     RMQRNMLATLACSLGVPMLSHGDEIGRTQHGNNNAYCQDNPISWVDWNLTPARRELLE
                     FTRLLFRFRAATPLLRTRLFPGSEAETGPLQLTWHRPDGELMTVDDWSHGHSHAMAML
                     LRDRSEAILLALNAGGRSRPFVLPALDVNGDWLEVLHTAQTEGDLTRGTVAVGPRSLV
                     VLRFEATG"
     gene            complement(2107..2523)
                     /locus_tag="VFB89_01465"
     CDS             complement(2107..2523)
                     /locus_tag="VFB89_01465"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM25999.1"
                     /translation="MKAPPLMLLLGRLVFDVPLRGNLAALYGVTFAFIVANLVLGLLV
                     STAVKTQTQAMQLSFFFFLPNILLSGFMFPREAMPAAAQWLGALLPLTYYLRILRGIL
                     LRGVGPTQLWAESLLLLGFAVVLVVLSVRRFSKTIE"
     gene            complement(2611..3573)
                     /locus_tag="VFB89_01470"
     CDS             complement(2611..3573)
                     /locus_tag="VFB89_01470"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HZM26000.1"
                     /translation="MLPIRQLRSSSLVIGVIVGSASGAPPQGNAQQAGLSAAEYGAAR
                     AQLLAADSALARVIHSKGEAPGFSSTLTDDAVYLEPGADHVRGKDRIGTFLSTRSTKR
                     KLSIRPAFGEISADGSVGYTVGWTTLTDSAAATPQYGKYIAFWRRRPDGAWQVEVWNR
                     SRAAEPPPRSPALPAGQLQRTAQLRAVDPVAASRQMRSADSAFAALSVAGGAAPAFYQ
                     YAAPQAIALGNGKDFVIGPAAIRDDQAAGAVAGQTLDWKPVAAGAGVSGDLGWTVGEF
                     RFTAPKKGKTLTVAGKYLTIWSRMPDGSWRYVADGGSASAPPKP"
     gene            complement(4075..4650)
                     /locus_tag="VFB89_01475"
     CDS             complement(4075..4650)
                     /locus_tag="VFB89_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26001.1"
                     /translation="MRSLFLLSSLLAIAACAGAVQGDPPDSSFSPTARSAEDSIPPAL
                     APANTGGAAPGTSGDVATTSPATAHPSQATTAASRDPVTQTRSGRFRAELLQDKEFRL
                     VFGDLQRLKVVLDFHESRWGVLKLMVGPGYTTVSSAEFNLEHLYRAYKAASYYSNDVV
                     VELWKDGTKLGEVTDDGVLVGPDYKVPRQPY"
     gene            5014..5757
                     /locus_tag="VFB89_01480"
     CDS             5014..5757
                     /locus_tag="VFB89_01480"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HZM26002.1"
                     /translation="MDPRTVSQIFGVALLLVALAGAPALEAVSAQAPPDSVLYQLHPS
                     SRLVVKTGKAGFFGFAGHTHVIRARAVSGMLVYYPGQPISHLQLKVPTDSLEVLTPPD
                     TAEIRKVTEAMRIETLHVDRYPEMTFSADSLTARSGKMEMQLALTMHGTTRRVPVTAD
                     LAIGSDTIRATGTFTAKQSDFGIKPFSGGPAGTVKVADRVTFCFDLVAIRATTGVRGD
                     QAAAGTDARTVPGCVDLAKAPSDTARRPM"
     gene            complement(5844..6680)
                     /locus_tag="VFB89_01485"
     CDS             complement(5844..6680)
                     /locus_tag="VFB89_01485"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZM26003.1"
                     /translation="MTEIPPLLTEALRDRYLIEREVGHGAMATVFLAHDVRQQQPVAI
                     KIMHRNLSASLDVERFRREMGIAASLSHPSIVPLFESGNVCGVPYYSMPYIEGGSLFQ
                     RLARERQLSLDEALRITHEVADALGYAHGFGILHRDVKPENILLANTRALVADFGLAR
                     AIGLANYRKLTDTGIMVGSVFYISPEQLLGERDLDQRADIYSLGCLLYEMLTGEPPYA
                     GPVAQGVFTRILRKPVPSARRLRATVPDTVDEALGRALAKSAAERFTSMAEFAAALPS
                     HG"
BASE COUNT         1329 a         2197 c         2177 g         1198 t
ORIGIN      
        1 cgatccggcg ggcaccgtca tccagtggcc tcgaagcgga gcacgaccag cgagcgcgga
       61 cccaccgcaa cggtcccccg agtcagatca ccctctgtct gtgccgtgtg cagcacttcg
      121 agccaatcgc cgttgacatc gagggcggga agtacgaacg gccgggatcg tccaccggca
      181 ttgagcgcga gcaggattgc ttcggagcgg tcccgcagca gcatcgccat cgcatggcta
      241 tgaccgtggc tccaatcatc cacggtcatg agctctccgt ccggccggtg ccacgtgagc
      301 tgcaacgggc cggtttcagc ctcagaaccg ggaaaaagac gtgtccgaag cagcggcgtc
      361 gcagcccgaa agcggaacag taaacgggtg aactcgagca gctctcggcg cgcaggcgtg
      421 aggttccagt cgacccaact gatcgggttg tcctggcagt acgcgttatt gttgccgtgc
      481 tgggtgcgtc cgatttcgtc gccgtgcgag agcatgggca cgccgagcga gcacgccagg
      541 gtggcgagca tgttgcgttg catgcgctcg cgaactcggg cgatgtgcgg cgcgtccgtc
      601 gggccctcca ctccccagtt ccggctcaag ttatggtcgt ttccatcctg atttgcttca
      661 ccgttggcct cgttgtgctt ccgttcgtag ctcaccagat cgttgagcgt gaacccatcg
      721 tggcaggtga cgaagttcac gctcgcctgt ggtgtgcggt cggccggtcc gaatagatct
      781 gcactcccgg cgagccgggt tgcgagctcg ggaagaagtc ccggatcgcc ccgccaaaac
      841 ccgcgcacgg cgtcgcgata cttcccattc cagtccgccc aacccgctgg aaaggctccc
      901 agttgatacc cattcgggcc cagatcccag ggctctgcaa tgagcttcac gtcggcaagg
      961 accgggtcct gccgcacaac ctcgaaaaaa cgggcctgcg cgttgaactc gtccccagga
     1021 tctcggccga gtatcggacc gagatcgaaa cggaatccat ccacgtgcat gtcctgcacc
     1081 caatagcgca ggctgtcgag caccaatcgg agcgcctgag gacggcgaat atccagcgtg
     1141 tcgccgcagc cggtgaagtc caggtaatgg cgcggattgg tgggatcgag acgatagtac
     1201 cccgcgttgt ccacgccgcg gagacacagc gtcggcccca ggtgattgcc ctccgcggtg
     1261 tggttgaaga ccatgtccag gatgacctcg atgccaaacc gatgaaaccg cttgaccatg
     1321 gtcttgaact cggtcacttg ctggccgagg cggccgctgg catagcgcac gtcgggggcg
     1381 aagtatccga tcgtgttgta gccccaataa ttgaccagtc cccgatcact gatcgtgcgc
     1441 tcggtcacga aatggcagat cggcagaagc tccaccgctg tcacgtccag acttttcaga
     1501 tggttgatca ccgcgtccgc tgccaggccg agataggtcc cccgaagcgg ggcggggacc
     1561 tcgggatgac gcatggtcat ccccttcacg tgacattcgt agatcacggt gtcggcccat
     1621 ggcgtccgcg gcgggcgatc atcggcccag gtgaacgccg actccaccac catgcatttg
     1681 ggaagtccgc ctgcactgtc ccgcggatcg gggatcaggt cctgttcggg atgccccaca
     1741 acgtagcccg acagcgcatc ggtccagcgg acggagccgg taatggcgcg cgcatacgga
     1801 tcgagcagca gcttggcggg attgaagcgg tggccggtct gcggagacca tggtccgtgc
     1861 acccggtagc catagagctg ccccggttgc gcatcgggca ggtaggtatg ccacacgatg
     1921 ccggtgcgct cccgaacggg aacccgcagc gcctcccggg tgtcgtcggg gccctcgaaa
     1981 agacacacct cgacggcggt ggcatgctcc gacgaaagcg cgaagttggt cccttcgccg
     2041 tcccaatgag cccccaagac ggccggggag ccgggccgaa cgcggatcat gcggtcaagt
     2101 gtttggtcac tcgatggtct tggagaaccg ccgaacgctc aggacgacga ggacaaccgc
     2161 gaaacccaga aggaggaggc tctccgccca gagctgcgtc ggccccaccc cccgcaacaa
     2221 gataccccgc agtatccgca gatagtacgt gagcggcaag agcgcgccca gccattgcgc
     2281 cgccgccggc atcgcctccc gcgggaacat gaagccggac aacaggatgt tgggaaggaa
     2341 gaagaagaag ctgagctgca tggcctgggt ctgcgtcttt accgcagtcg agaccagaag
     2401 cccgagcacc agattggcga cgatgaaggc gaacgttacg ccgtagaggg cggcaagatt
     2461 cccgcgtagc ggcacatcga agaccaatcg ccccaacagt agcatgaggg gcggggcttt
     2521 catggcaggt agataattaa tcgattagtt actccgggca agcacgcgat cgacttccgg
     2581 cacagggatg gatctcctag cttcggatat ttagggtttg ggtggcgcgc tggcgctgcc
     2641 gccgtctgcc acgtagcgcc aggagccatc cggcatgcgg ctccagatgg tgaggtactt
     2701 gccagccacg gtcagcgtct tccccttctt cggcgcggta aagcggaatt cacccactgt
     2761 ccagcccaga tcgccggaca ctcctgcccc ggctgccacc ggtttccagt cgagagtctg
     2821 gccggccact gcgccagccg cctgatcgtc gcggatggcc gccggaccga ttacgaaatc
     2881 tttgccgttc cccaacgcaa ttgcctgagg cgcggcgtat tggtaaaacg ccggcgcggc
     2941 gccacccgcg accgacagcg cggcgaacgc cgagtcggcg ctccgcattt gtcggctggc
     3001 agcaaccggg tcgaccgccc gtagctgagc tgtccgctga agctgtccgg ctggaagcgc
     3061 tgggctgcgg ggcggcggct ccgctgcgcg acttcggttc cacacctcga cctgccaggc
     3121 tccatccggg cggcgacgcc agaacgcgat gtacttcccg tactgcggcg tggctgcagc
     3181 gctgtccgtg agtgtggtcc agcccaccgt atatcccacg cttccgtcgg cggagatttc
     3241 gccgaaggcc ggccggatgc tgagctttcg cttggtcgat cgagtggata ggaaggtccc
     3301 gattcgatcc ttcccacgga catggtccgc accgggctcg agataaaccg catcgtccgt
     3361 caacgtgctg gagaagccag gcgcctcccc tttcgagtgg atgactcgcg cgagcgcgga
     3421 atcagcggcg agcaattgag cccgggcagc cccgtactcg gctgcgctca atccagcctg
     3481 ttgagcgttg ccctgcggcg gtgccccgga cgcagagcca acgatgacgc caatgaccag
     3541 ggaactcgat cgaagctgac gaatcgggag cattgggctt ccctacggtc gatcactacc
     3601 gttggtgcca gtctcgccca aaaaacacaa cgcccggccg ggtgcattgc acccaccggg
     3661 gcgaaggctc ttggcagatt gatcacccgg gctgctctga ttccccgggg cccgaaggct
     3721 atgtccttac ccgaacttta gtcgcgcgca gggctgttca caagtcccga tgatgtcgtg
     3781 gcgccgcgtg ctaccggcgc ttgggacgtc ccgccagcaa tggcgtcatc caacgtttgg
     3841 gtgcgctgcc cgctgcaatc aactggctca ggaacgaacg gaccgagcgc acgctggaac
     3901 gaaaccattg cagcagcagg tccaggcgcc tgcggcgccg ccaatacgtc cgcggcaaga
     3961 gaatcacaac gcaaacactc agggccgaac tgcgaccaat agggataaat gtcagactgc
     4021 gtctcaaaga tacactgtct gcgatgatat cgagggtgag attgctcaca aacctcaata
     4081 gggctggcgt ggaaccttgt aatccggccc taccagaacg ccgtcgtccg tgacctcgcc
     4141 cagttttgta ccgtccttcc acagctccac caccacatcg ttgctgtagt acgacgctgc
     4201 cttataggcc cgatagaggt gctcgaggtt gaactctgcc gacgagaccg tcgtgtagcc
     4261 ggggccgacc atcagcttga gcacgcccca acgggactcg tgaaagtcca gcaccacctt
     4321 caggcgctgc aaatcgccga ataccagccg aaattccttg tcctgcagca actctgctcg
     4381 aaagcgtccg ctccgggtct gtgttaccgg atcccggctg gcagccgtgg ttgcctgcga
     4441 ggggtgggct gtggcgggac tcgtcgtggc gacatcgccg gacgtgccgg gcgcggcgcc
     4501 tccggtattc gccggcgcca acgcgggcgg gatcgaatcc tctgccgacc gagcggtggg
     4561 actgaacgac gagtctgggg gatcgccctg gaccgcgccg gcacacgcgg cgatggccag
     4621 aaggctactc agcaggaaaa gcgagcgcat ccggtgatct cctggaccgt cggctcgacg
     4681 ccggatgtta accggtgcgg gggggcacgc acccatgggt tggactcggg gctccgggcg
     4741 atccgagtca tggccgctca tgcgaataag ggggtgttca aagagaaagc tatgcaagag
     4801 cctagccaca gccaaatccg tcgaccggcc cggctctgta cccccttatc gttctcgatg
     4861 cggcattcac tccacaccag tgcctctcca gagcgatgtc gaggcggctg ccgccggacc
     4921 aatcctggga tcactgtcct cgagatggac ggtgtctatc cttgtgaggt agttggcccg
     4981 aagcgcgaat tgggcagaaa tagacgagga gcaatggacc cacgcacagt gagccagata
     5041 ttcggcgttg cgctgttgct cgtagcgctc gcgggggcgc cagctctgga ggccgtgagt
     5101 gcgcaagctc cgcccgactc ggttctgtac cagcttcatc caagcagtcg tttggtggtc
     5161 aagaccggga aggcaggctt ctttggcttc gctggccaca cccacgtcat tcgcgctcgg
     5221 gccgtatcag gcatgctggt ttactacccc ggacagccga tctcgcacct ccagctcaag
     5281 gttccgaccg acagcctgga ggtgctaacg ccgccggata cagccgagat ccgcaaggtg
     5341 accgaggcca tgcgaatcga gactctgcac gtggatcgct accccgagat gactttctct
     5401 gctgactccc tcaccgcgcg gagcggcaaa atggagatgc aactcgcgct caccatgcac
     5461 ggcaccacac gccgggttcc ggtgaccgcg gatttagcca tcggctccga cactatcagg
     5521 gccaccggca catttaccgc taaacaaagc gacttcggga ttaagccctt cagcggcgga
     5581 cctgccggca cggtcaaagt ggccgaccgg gtgacgttct gttttgacct ggtggcgata
     5641 cgagcaacga caggggtccg cggggatcag gcggcggcgg gcaccgatgc gcgcacggtg
     5701 ccgggctgcg ttgacctcgc caaggcgccc tcggataccg cacgtcgccc aatgtagcgg
     5761 ccggagcgtg tgatggcctg gaggctaatc catggatcgg cggcaccgaa catcaagact
     5821 aaccatccga agaaggtccg ctgttaccca tgggagggaa gcgcggcggc aaattccgcc
     5881 atgctcgtga atctctcggc agctgatttg gcgagcgcac gacctagagc ctcatcgacg
     5941 gtgtccggca cagtggcccg gagccgccga gccgagggca cgggttttcg taagatccgg
     6001 gtgaacaccc cttgggccac cggcccggcg taggggggtt cgccggtcaa catctcatac
     6061 aaaaggcagc ccagactgta gatgtcggcc cgctggtcca ggtcgcgctc gccaagcagt
     6121 tgctccgggc ttatgtaaaa cacgctcccc accatgattc cggtgtccgt cagcttgcga
     6181 tagttcgcga gaccgatggc cctggccagg ccgaaatccg caaccagcgc tcgggtattg
     6241 gcaaggagga tgttttccgg cttcacgtcc cggtgcagga tgccgaagcc gtgggcgtag
     6301 ccgagcgcgt cagccacttc gtgcgtgatc cggagggcct catccaaaga gagctgacgc
     6361 tcccgcgcca gccgctgaaa aagtgagccg ccttcgatgt acggcatgct gtagtagggc
     6421 accccgcaca catttccaga ctcaaacaga ggcacgatgc tggggtgcga gagcgaggcg
     6481 gcgatgccca tctcgcggcg aaaccgctcg acgtcaagcg aagcgctcag attgcggtgc
     6541 atgatcttga tggctaccgg ctgttgctgg cggacgtcgt gcgcgaggaa cacagtggcc
     6601 atcgcaccgt gcccgacctc gcgctctatg agatagcggt cgcggagggc ctcagtgagt
     6661 agtgggggta tttccgtcac accggctacc gacgacaggc aatgattttg cgaggtgaac
     6721 gttttctgac cgctcagcgt accatcttcc gaacctagtc cccataaacc gcaatgaccg
     6781 gcggggaaat cccgttcgtg ctgtcggaaa gcgcgccccc ctgatctcca gcgtccgcgg
     6841 cgtcctcccc caaacacaaa gaacgcctta cacgcgaagg gggtcgcgga ggagggcggg
     6901 a
//
LOCUS       DASRJQ010000063         2195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3056141, whole genome shotgun sequence.
ACCESSION   DASRJQ010000063 DASRJQ010000000
VERSION     DASRJQ010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3056141"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="VFB89_01490"
     CDS             <1..824
                     /locus_tag="VFB89_01490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HZM26004.1"
                     /translation="KPDRVVVGVDSEPARETMQELYAPFVRTGNPIIFMDIASAEVTK
                     YAANAMLATRISFMNQVAEFCERVGADVKQVRQGIGSDRRIGPAFLFPGPGYGGSCFP
                     KDVKALIRSGTDVGVRFDLLESVEAVNERQKLLLFRKLVKALGENLSGTRIGIWGLAF
                     KAGTDDMRESPSVPLIRSLVGRGATVLAHDPEAHETARAVFSNTVQYVKDPYDAIEDA
                     SALVIVTEWLVYRNPDFERMKKALRRPLVIDGRNLYDAARMRSLGFQYHGIGTPA"
     gene            821..1756
                     /locus_tag="VFB89_01495"
     CDS             821..1756
                     /locus_tag="VFB89_01495"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HZM26005.1"
                     /translation="MRVLITGSAGFLGSHLADRFIAEGHHVIAMDNFITGTPENIAHL
                     MGHERFRFVQHDVTNYLYVEGTLDGVLHFASPASPIDYLELPIQTLKVGSLGSHKALG
                     LAKAKGARFLLASTSEVYGDPQVHPQPESYWGHVNPVGPRGVYDEAKRFAEALTMAYH
                     RYHGLETRIVRIFNTYGPRMRARDGRVVSNFIVQALKGEPLTIYGDGSQTRSFCYVSD
                     LVEGIYRLFESDRIEPTNIGNPIEFTVRALAERVLQLTGARSKLVERPLPTDDPKVRQ
                     PDISIAQQLLKWEPQVSLDEGLERTIGFFRTKLAA"
     gene            1753..>2195
                     /locus_tag="VFB89_01500"
     CDS             1753..>2195
                     /locus_tag="VFB89_01500"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZM26006.1"
                     /translation="MTRALVTGAAGFIGSHLVEALIARGDDVIGVDNFDPFYPRPMKE
                     RNLREIGERPGFCFHELDMLEVEPLARLLSPECVVVHLAGKAGVRPSIADPVGYTRAN
                     VSGTAAVLEAARRAGVRRLVYGSSSSVYGNSTPTPFREDAVAIEP"
BASE COUNT          390 a          698 c          712 g          395 t
ORIGIN      
        1 tgaaacccga ccgagtggtg gtcggagtgg actcggaacc ggcgcgggaa acgatgcagg
       61 agctctatgc gccgttcgtg cgcaccggaa atcccatcat tttcatggac atcgcgtcag
      121 ccgaggtcac caagtacgcc gccaacgcaa tgctcgccac ccggatttcg ttcatgaatc
      181 aggtcgccga gttctgcgag cgcgtcggag ccgacgtgaa gcaggtccgt cagggcatcg
      241 gcagcgatcg acgtatcggt cccgcgtttc tgttcccggg tcctggatac ggaggatcgt
      301 gctttccgaa ggacgtgaag gcgctgatcc ggtcgggcac cgacgtcggg gtccgcttcg
      361 acctcctcga atcggtcgag gcggtgaacg aacgccagaa gctcctcttg tttcgcaagc
      421 tggtgaaggc acttggcgaa aacctctccg gcaccaggat cgggatctgg ggactcgcct
      481 tcaaggcggg gacggacgac atgcgggaaa gcccctcggt gccgctgatc cggtcgctgg
      541 tcggtcgggg agcgaccgtg ctcgctcacg acccggaagc acacgagacc gcgcgcgcgg
      601 tcttctccaa caccgtgcag tacgtgaaag acccctatga cgccatcgaa gatgccagcg
      661 cgctcgtgat tgtcaccgag tggctggtgt accgcaaccc ggactttgag cgaatgaaaa
      721 aggcgttgcg gcgtcccctc gtgatcgacg gtcggaactt gtacgacgcg gcgcgaatgc
      781 ggtcgctggg cttccaatac cacggaatcg ggacgcccgc gtgagagtgc tcatcaccgg
      841 ctcggccggt ttcctgggct cgcatctggc ggatcgcttc attgcggagg gccaccatgt
      901 catcgcgatg gacaacttca tcaccgggac tcccgagaac atcgcgcacc tgatggggca
      961 cgagcgcttt cgcttcgtcc agcacgacgt caccaactat ctgtatgtcg agggcacgct
     1021 cgacggcgtg ctccatttcg cgtcaccggc gagccccatc gactatctcg agctgcccat
     1081 ccagacgctc aaggtcggct ccctcggcag ccacaaggcg ctgggtctcg cgaaggcgaa
     1141 gggcgcacga ttcctgctgg cgtccacgtc cgaggtgtac ggcgatccgc aggtgcatcc
     1201 ccagccggag agctactggg ggcacgtgaa tccggtcggc ccccgtggcg tgtacgacga
     1261 ggccaagcgg tttgctgagg cgctcaccat ggcctaccac cggtaccacg gcctcgaaac
     1321 gcggatcgtg cggatcttca acacgtacgg gccgcggatg cgggcccggg acggacgggt
     1381 cgtgtccaat ttcattgttc aggcgctgaa gggtgaaccg ctcacgatct acggtgatgg
     1441 ctcccagacc cgatcgttct gctatgtgtc ggatctggtc gaagggatct accgtctctt
     1501 cgaaagcgat cgcatcgagc cgaccaacat cggcaacccg atcgaattca cggtgagagc
     1561 cctggccgag cgggtcctgc agctcacggg cgcacgctcg aagctggtcg agcgtccgct
     1621 gcccaccgac gatcccaagg tccgccagcc ggacatctcg atcgcccaac agctgctcaa
     1681 gtgggaacct caggtgagcc tcgacgaagg actggagcgg accatcgggt tcttccggac
     1741 gaagcttgcg gcatgactcg ggccctggtg accggagcag ccgggttcat cggctcccac
     1801 ctggtcgagg cgctgattgc ccgcggcgac gacgtgatcg gagtcgacaa cttcgatccg
     1861 ttttatccgc gtccaatgaa ggagcggaac cttcgggaga tcggggagcg ccccggattc
     1921 tgctttcacg agctggacat gctcgaggtc gagccgcttg cccggttgct ctcgcccgag
     1981 tgcgtggtgg ttcacctcgc ggggaaggct ggcgtgcggc cctccatcgc ggatccggtg
     2041 gggtacaccc gcgccaatgt cagcggcacg gccgcggttc tcgaggcggc tcgccgcgcc
     2101 ggtgtgcggc ggctggtcta cggctcgtcc tcctcggtgt acggcaacag cacaccgacc
     2161 ccttttcgcg aagatgcggt tgcgatcgaa ccgat
//
LOCUS       DASRJQ010000064         2642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3055710, whole genome shotgun sequence.
ACCESSION   DASRJQ010000064 DASRJQ010000000
VERSION     DASRJQ010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3055710"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VFB89_01505"
     CDS             complement(<1..215)
                     /locus_tag="VFB89_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26007.1"
                     /translation="METANQGALQAALGARYTLERELGRGGMATVYLARDLRHRRSVA
                     VKVLHPELSAVLGPERFLREIELTANL"
     gene            complement(1197..2510)
                     /locus_tag="VFB89_01510"
     CDS             complement(1197..2510)
                     /locus_tag="VFB89_01510"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZM26008.1"
                     /translation="MSTSLPESVAPRNADPSERTALAKVTWRLLPFLFLLFMVCFLDR
                     VNVGFAALQMNRDLGLSPAVYGFGAGIFFLGYVLCEVPSNLLLARMGARVWIARIMIT
                     WGLLASAMMFVRGPLSLYGLRFLLGAAEAGFAPGIVYYLSRWFPAAERARAVARFMAA
                     VPLAGAVGGPLSGALLSLNGHLGLAGWQWLFLLEGVPAVVLGFVVLVYLTERPEEATW
                     LTPTERAWLTTHLSREREQCERMSVLQALSNGTVWRLGLLGFAFGFSGLGLWLPQIVR
                     GLSGLSDFMVGLVSAVPYLVAGAAMVLVGAHSDRSGERLLHIAASATAAACGFLASAY
                     LHSPVLAVLALSLAAAGLSSAQGPFWALPSAFLSGPAAAGGIALINSVANLSGFVDPY
                     LIGVLKGATGNFDSGLLLLALVPLAGASLALRLRRAPGSRPWLER"
BASE COUNT          542 a          871 c          876 g          353 t
ORIGIN      
        1 tggagattgg cggtaagctc gatctcgcgt agaaaccgct cgggccccag cacggccgac
       61 agctcggggt gcaacacctt gaccgcgacg ctgcggcggt ggcgcaggtc gcgcgcgagg
      121 tagacggtcg ccatcccgcc ccgccccagc tcccgctcca gcgtgtaacg cgccccgagc
      181 gccgcctgca gcgcgccttg gttcgccgtc tccaccgagg cctcccgatc gacgccgacg
      241 gaacatacga cgcccttccc gccccgacca ctccgcccac ttgttttcgg ccgcaggtcg
      301 gattccggct gcggctcatg tccgagcccg gcgaggaccc gccgacctga cagagcagag
      361 cgtcgcgggg gtcggaggac tccgaactgg accgcggcgc cacggcggtt cgcggtcgaa
      421 aaccgtcggt tcgaaagcct aacgctcgac accccgtcgc gccgtgcgtt cgagacctcc
      481 caatcaatca gaaaccggat gagcgcgatg tagcccgcga aatcatagcg caaaccctag
      541 taggttcctc gactaacgaa aaacggtcct gcaacgtcgg atgttgcagg accgttaggc
      601 tggaatgggg gcgggtgaat tcaagccacg gacgtcctgg ttaacggccg gtggctagac
      661 agatcgacag acgatggaga cgtggtatcg acacgcggac gacgcaggcg tgctggctgc
      721 gatggaagtc gaccacgaag ctgcgtgagc gcaggctcgg aacctcgacg tgactaactg
      781 cggcgaaact acggcgagcc tctcgagaac gaaaaagtcc cgcacgcgta aacgcatgcg
      841 ggacattcac tggggcgggt ggattcgaac caccgacctc ctgattaaca gtcggggtag
      901 ttaggcatct actccgggta acctgccggg ttacggccac ctaacgagca tcggcgcccg
      961 ctcaggatgg cgaaaatgcc cgctcatgtc cggagaaact acggcgaaac tgcggcaaag
     1021 ccgatgaatc gacggcgcta gccaaggggc actctcatgc cctttggtgt cggtgcgcgg
     1081 cgccgctacg tacgtccact tccttggtgc cgtcaaaact cgggatcatc caaaagcacc
     1141 taatctgccc ttatcagatg cacccttgag tctgacctag ggcacgcctg gcgtcgctac
     1201 cgttccagcc acggtcgtga gcccggggct cgtcggaggc ggagggccag cgaggcgccg
     1261 gccaatggca cgagcgcgag gagtaggagg ccgctgtcga agttcccggt ggcacccttg
     1321 aggaccccaa tcaagtaggg atcgacgaag ccgctcaggt tcgccaccga gttaatcagg
     1381 gcgatgccac cggccgcggc tgggccgctc aagaaggccg acggaagcgc ccaaaaggga
     1441 ccctgagcgc tcgacaggcc cgccgccgcg agcgacagcg cgagcacggc aagcaccggc
     1501 gagtgcaagt acgcgctcgc caggaagccg catgccgccg ctgttgcgga cgccgcgatg
     1561 tggaggaggc gttcgccgct acggtccgag tgtgcgccca cgagcaccat ggcggcgccg
     1621 gcgacgaggt acggcacggc cgagacgagg ccgaccatga aatcgctcag tccggagagt
     1681 cccctcacga tctgcggcag ccaaaggccc agcccggaaa atccgaacgc aaagccgagc
     1741 agacccagcc gccacacggt gccgttcgag agcgcctgga gcacgctcat tcgctcacat
     1801 tgctcgcgct cacggctcag gtgggtggtg agccacgcgc gctcagtcgg cgtaagccat
     1861 gttgcctctt cggggcgctc ggtcagatac accaaaacga cgaagccgag cacaaccgcg
     1921 ggcacacctt cgaggaggaa aagccactgc cagccggcga ggccgaggtg cccattgagg
     1981 ctgagcagcg cccccgagag gggaccgccg acggccccgg cgagcggaac cgcggccatg
     2041 aaccgggcga ctgcccgcgc ccgctccgcg gccggaaacc atctgctgag gtagtagact
     2101 atgcccgggg cgaatcccgc ttcggccgcg ccgagcagga accggagccc gtacaagctc
     2161 agcgggcccc ggacgaacat catcgctgac gccaggagtc cccatgtgat catgatgcgg
     2221 gcgatccaca cgcgcgcgcc catccgcgcc aagaggaggt tgctcggcac ctcgcagagg
     2281 acgtagccca ggaagaagat cccggcaccg aagccgtaga cggcggggct caagcccagg
     2341 tcgcggttca tctggagcgc ggcgaacccg acattcaccc ggtccaggaa gcacaccatg
     2401 aaaaggagga acaggaacgg caggagccgc cacgtcacct tggcgagcgc cgttcgttcc
     2461 gagggatccg cgttgcgcgg ggccaccgac tccggcagag aagtggacat gggcggcgct
     2521 ggtgagagca gatccaacat agagagctgg acaccgtgcg aaagagcggc gcacggatca
     2581 ggagagcgct cgacagggca agctgctcaa aggacccggt gggtgggccg cggtcggtgc
     2641 ct
//
LOCUS       DASRJQ010000065         4315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1363314, whole genome shotgun sequence.
ACCESSION   DASRJQ010000065 DASRJQ010000000
VERSION     DASRJQ010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363314"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            140..1411
                     /locus_tag="VFB89_01515"
     CDS             140..1411
                     /locus_tag="VFB89_01515"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HZM26009.1"
                     /translation="MATHSDPEARFLPARPNLRHLRDQAKDLLRAGKAASTSEAQLKI
                     AREYGFPSWPKLKAHVDSLTEIGRLKEAIDTEDLDRVRAMMTRNPALHRAPLGYGNNG
                     PLTWVAECRGVEGPPSPARLEMARWMIENGSDVHQGGDGPLMRASLRERIPMLELLVN
                     HGADVNALWDGHYPIVLGPCECLAPGALRWLLENGADPKRVSARYGGALSMLLCTYGR
                     DAAGKHACLELLAGAGYEIPDTPALALHRGRTDLLEAHLRRDSGLLGRRFTEAEIYPP
                     ELGIQPGDGLTATPTAGGTLLHLAIEYDDLDTAAWLVARGADVNARAAVDEEGFGGHT
                     PLYHAVVTLGRHDDAKARFLLERGADPNARTTLRKQLRDMGDAEKERLHTFHDVTPIA
                     YARAFLEPRWVSEPALAAIAEYGGTARAGAG"
     gene            complement(1438..3591)
                     /locus_tag="VFB89_01520"
     CDS             complement(1438..3591)
                     /locus_tag="VFB89_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HZM26010.1"
                     /translation="MMKTTLSAAVLISILWGPVCLAQTAPATEVDDFKPASSNAPGSQ
                     YPQVNSEGRAKFRVNAPNAKNVVVSLRKTALTQGEDGVWTGTTEPLDPGFHYYQIIVD
                     GFGVADPSSDTFFGVSDHRSGIEIPERGVDFYDAKDVPHGDVRLKWYFSKITNAWRRC
                     FIYTPPDYDKNTGARYPVLYLQHGAGEDETAWSRQGRMNFILDNLIAEGKANPMIVVM
                     DNGGGSALFANRAGAEGRGTPGAPPGVGGAGGPAGAQAGEPKQTGGVASAAPGGRGAG
                     RGAFGGQQFSEILLTEIIPMVESNLRTFPDREHRAIAGLSMGAGQATRIGASNLDKFA
                     YIAGFSGGGLRLEGESAVDPNEFNKMVKVFYLSMGTKENIDRFRQTCAALKEAGIKYL
                     AYEAPGTAHEFQTWRKSLHGFAQLLFKDDGNSSPATSAQAQPAAPRGDSASPDARRRG
                     GFGGPIELGPDDKPAFADPPAGFNAKREDVPHGDLAMVEYDSKTVGTRRKMLIYTPPG
                     YSADRKYPVLYLLHGIGGEETEWQRLCHPENIIDNLLADAKIQPMILVMPNGRAQVND
                     RPEGNIFAAAPAFANFEGDLLKDVIPAIEAKYSVETNRENRALAGLSMGGGQSLNFGL
                     AHLETFAWIGGFSSAPNTQPPAELLADPVAAKEKLKLLWLSCGNKDGLIRVSQGVHRY
                     LKEKDIPHVWHVDANGHDPTEWANSLYLFAQRIFR"
     gene            complement(3647..4111)
                     /locus_tag="VFB89_01525"
     CDS             complement(3647..4111)
                     /locus_tag="VFB89_01525"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZM26011.1"
                     /translation="MATAQILPLQLRAAYLAMHRQTNACLAESGCTADQFVLLGLLAR
                     EDAVTQQELVRRASSDPNTVRAMLVLLEKRGLIARVGHPTDGRARSVTLTGKGRSLHR
                     KQLADTEPLRERLRALFSLNEADALVEYLSRISEALSPPQRELASKVKGEAR"
BASE COUNT          778 a         1363 c         1348 g          826 t
ORIGIN      
        1 cgcgtacatt cgttgcagcg cgcagggcgc atcccgtccg cggaaagggt agaagcccac
       61 ctgttctggt cggcgattca cgcaccttct ttcagcccgt gatgcggact tcgggcacgt
      121 tcaatgaaag gaggtcgcga tggcgactca ttccgacccc gaagcgcgct tcctgccggc
      181 ccgtcccaac ctccgccacc tgcgggacca ggcaaaagat ctccttcgcg cggggaaagc
      241 ggcgtcaact tcggaagccc agctcaagat cgcccgcgag tacgggtttc cgagctggcc
      301 gaaactgaag gcccacgtcg attccctgac cgagatcggc cggctcaagg aagcaatcga
      361 caccgaagac ctcgaccggg tcagggcgat gatgacccgc aatcccgcgc tacaccgcgc
      421 cccgctcggg tacggaaaca acgggccgtt gacctgggtc gccgagtgcc gcggcgtcga
      481 gggtccgccg agcccggcgc ggctggagat ggcccgatgg atgatcgaga acggctccga
      541 cgtccaccag ggcggagacg ggcccctgat gcgcgcctcg ctccgcgagc ggattccgat
      601 gctcgagctt ctggtaaatc acggcgcgga tgtcaacgcg ctctgggacg gacactaccc
      661 cattgtgctg gggccgtgcg agtgcctcgc gcccggagcc ctccggtggc tcttggaaaa
      721 tggcgcggac ccgaaacgcg tctctgctcg atacgggggc gcgctttcga tgctcctctg
      781 cacttatggt cgggatgctg ccggcaaaca tgcctgtctt gagctcctgg ccggagctgg
      841 atatgagatc ccggacacgc ccgccttggc gctccaccgg ggacgaaccg acctgttgga
      901 agctcatctc cggcgcgatt ccggtctcct cggccgacga ttcacggagg cggagattta
      961 cccgccggag cttgggatcc agcctggcga cggcctgacc gccactccca ccgccggcgg
     1021 cacgctcttg cacctcgcga tcgagtacga cgatctcgac acggccgcct ggctcgtggc
     1081 gcgaggcgcc gacgtaaacg cgagggctgc cgtcgacgag gaaggattcg ggggacacac
     1141 gcctctctac cacgcggtcg tgacgctggg ccggcacgac gacgcgaaag cgcgattttt
     1201 gctcgagcgc ggggccgacc cgaacgcgag gacgacacta cggaagcagc tgcgggatat
     1261 gggcgatgcc gagaaggaac gcctgcatac ttttcacgac gtaacgccga tcgcctatgc
     1321 gcgcgcgttt ctggagccgc ggtgggtcag cgagccggcc ttggcggcga ttgccgaata
     1381 cggagggacc gccagagcag gcgctggata gagagataga tcatcggacc gatgcgacta
     1441 cctgaagatc cgctgggcaa acagatacag actgttggcc cattcggtag ggtcgtggcc
     1501 attggcatcg acgtgccata catgcggaat gtctttttct ttgagatatc tatggacgcc
     1561 ctgactgaca cgaatcaggc catccttatt gccacaggac aaccacaaga gcttcagttt
     1621 ctctttggca gccaccgggt cagccaacag ctcggcggga ggttgggtgt tgggcgcgga
     1681 tgagaacccg ccgatccatg cgaatgtttc caggtgagcg aggccgaagt tcagggactg
     1741 gccgcccccc atcgatagcc ccgccagggc cctgttctcg cggttcgtct ccacggagta
     1801 cttggcctcg atggccggaa tcacgtcctt gagcaaatca ccctcgaaat tggcgaaagc
     1861 aggggctgcg gcaaagatat tcccttcggg gcggtcattg acctgggccc tgccgttggg
     1921 catgaccaag atcattggtt gaatcttcgc gtcagccagg aggttgtcga tgatgttctc
     1981 cgggtggcag aggcgctgcc attccgtctc ctcgccgccg atgccgtgca ataaataaag
     2041 cacagggtat ttgcgatcgg ccgaataacc cggcggggtg tagatgagca tttttcgacg
     2101 tgtgccgacc gtcttggagt catactcaac catcgccaga tcgccgtgag gaacatcctc
     2161 gcgcttggca ttgaaccccg cgggcggatc ggcgaaggcg ggtttgtcat ccgggcccag
     2221 ctcgatcggt ccgccgaagc cgccgcgtct acgagcatct ggcgaggctg aatcgccccg
     2281 tggagcggcc ggctgggcct gcgccgaagt tgccggcgag gagttgccat catccttgaa
     2341 cagcaattgg gcgaatccgt ggaggctctt tcgccatgtc tggaattcgt gagccgtgcc
     2401 gggtgcttcg taggccaggt acttgatccc cgcctccttc agtgctgcgc acgtctgtcg
     2461 gaaccgatcg atgttctcct tggtgcccat gctcaggtag aacaccttca ccatcttgtt
     2521 gaactcgttc ggatcgacgg cggattcacc ctcgagacgg aggccgcccc cgctgaaacc
     2581 agcgatgtag gcgaacttgt ccagattcga tgctccgatc cgagttgcct ggcctgcgcc
     2641 catcgaaagt ccggcgatgg cacggtgctc acggtctggg aaagtgcgga gattggactc
     2701 aaccatagga ataatctcag tcagcagaat ctcagaaaac tgctgccccc cgaatgcgcc
     2761 gcgaccggct cctcgcccgc cgggcgcggc tgacgccact cctccagttt gctttggttc
     2821 cccagcctga gctcccgcag gcccgcctgc tcctccaacc cccggcgggg ctccgggtgt
     2881 accccttcct tctgcgccag cccgattggc gaacagcgca ctgccaccgc cattgtccat
     2941 gacgacgatc atcgggttgg cttttccttc tgcgatcagg ttgtcgagta tgaagttcat
     3001 ccgtccctgc ctggaccagg ctgtctcgtc ttctcctgct ccgtgctgga ggtagagcac
     3061 cggataacgg gcgccggtgt tcttgtcgta gtcaggcggg gtatagatga agcatcgccg
     3121 ccaggcgttg gtgattttcg agaagtacca tttgagccgg acgtcgccgt gcgggacatc
     3181 cttggcgtca tagaagtcga cgcctcgttc cggaatctca atcccgctac ggtgatcact
     3241 gacgccgaag aaagtgtcgc tgctaggatc ggcgacgccg aaaccatcga caatgatctg
     3301 gtaatagtgg aagccaggat cgagcggttc ggtcgtgcca gtccacacgc catcctcgcc
     3361 ttgagtcagc gccgttttgc gaagactcac gacgacattc ttggcgttcg gggcattgac
     3421 gcggaacttc gcccgcccct cggaattgac ctgaggatac tgcgagccgg gggcattgct
     3481 ggaagcgggc ttgaaatcat cgacctccgt cgccggagcg gtctgcgcca ggcacaccgg
     3541 tccccagagt atggaaatca acacagcagc ggaaagcgtc gtcttcatca tgattctcca
     3601 ttagctccgt tttgccgacc atcgtcgacg agatcgtttc ggccgctcac cgagcttctc
     3661 ccttcacttt acttgcgagc tcacgctgcg gcggcgacag tgcctccgaa atgcgggaga
     3721 gatactcgac cagcgcatcc gcttcgttga ggctgaacaa ggcgcgcagg cgttcgcgga
     3781 gtggctcggt gtccgccaac tgcttgcgat ggaggcttcg ccccttgccc gtcagggtga
     3841 cgctgcgggc gcggccatcg gtgggatggc ccacccgcgc aatgagacct cgcttctcga
     3901 gcagcaccag catggcccgg accgtgttcg gatccgaaga ggctcggcgg accagctcct
     3961 gctgcgtgac cgcgtcctcg cgtgccagca ggcccaggag gacgaactgg tcggctgtgc
     4021 atccgctctc cgccaggcag gcattggtct ggcggtgcat ggcaaggtac gcggcgcgca
     4081 gctggagcgg gaggatttgt gcggtggcca taacgctatc gtaaatacgt atacgtagaa
     4141 tcacgagggg attctacgcc gtccccgggc cggaagtcca aggtacctcc ctgcgggccg
     4201 ccgcctcagc gccctcccgc ggaatggcgg cgatgcgcgc tgatctcgct ctgtccttcg
     4261 ctcacggttt gaaatcacct tctggtctgc cccggcggac catttcctgg cagta
//
LOCUS       DASRJQ010000066         4829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_526401, whole genome shotgun sequence.
ACCESSION   DASRJQ010000066 DASRJQ010000000
VERSION     DASRJQ010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526401"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1225)
                     /gene="ettA"
                     /locus_tag="VFB89_01530"
     CDS             complement(<1..1225)
                     /gene="ettA"
                     /locus_tag="VFB89_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HZM26012.1"
                     /translation="MAGEYIFTMRDLRKVVPPNKEILKGIYLAFYPGAKIGVLGANGA
                     GKSTLLRIMAGEDTDYLGDARPLPGIKIGYLPQEPRLDPAKDVRGNVEVAVAEQRDLL
                     DQFEQLSARFAEPMDDDEMNRLLEKQGNLQERIDALGLWELDHKIDLAMDALRLPPAE
                     ADVGKISGGERRRVALCQVLLAQPDMLLLDEPTNHLDAESVWWLERYLAEFPGTVVAV
                     THDRYFLDNVARWILELYQGAGIPWEGNYSSWLAQKQQRLAGEEKQASARQKTLAHEL
                     EWIRMAPRARQAKSKARITRYEELRAEAERQTEASSEILIPVPERLGDEVVIAKNVAK
                     GFGDQLLFEGLNFSLPRGGIVGVIGPNGAGKTTLFRIITGQEQPDAGDLVVGKTVQLA
                     YVDQSRESLDGNKTAY"
     gene            complement(1386..1718)
                     /locus_tag="VFB89_01535"
     CDS             complement(1386..1718)
                     /locus_tag="VFB89_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HZM26013.1"
                     /translation="MAAKKKSSKKAKRSTARRRPAKKKTAAKRKPNPAFMKAMRPSEQ
                     LAAVVGSGALPRTEVTKKLWAYIKRKGLQDKNNRRNIHADETLRPIFKKDVVNMFEMT
                     KLVNKHLS"
     gene            1878..3176
                     /locus_tag="VFB89_01540"
     CDS             1878..3176
                     /locus_tag="VFB89_01540"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZM26014.1"
                     /translation="MTPPVSTLMRARVLLPAVGALAIFGVFLRRPRLAPPRHSERPVC
                     APDNGGLNVPNGFCAVVVAHDVGSVRHLVVAPNGDIFANRERGGVIALRDTSGDGVAD
                     IVRPFNTQGGTGIALEGDFLYYSTDAAVYRYPWKSGQLEPKGEAESIVIDLPADGDHK
                     SKSIALGSGGALFVNIGSATNSCQQQNRASRSPGRDPCDELKTRAGIWRFDANRKDQR
                     LPDGEHWATGLRNTVALATDPKSRALYGVTHGRDQLRESWGFSAEKGAENPGEEMVRP
                     ERGDDYGWPYCYYDTDLKQKVLAPEYGGDGKQVGRCARMKPALVSFPGHWAPMALAFG
                     DGKFGPAYASGVFVAFHGSWNRAPLPQAGYRVVWQPMADGRPSGTYQSFVTGTKGPTS
                     IRPSGLAIGPDGSLYISADQNGTIWRVLNSSDAEHTKEGT"
     gene            3284..3769
                     /locus_tag="VFB89_01545"
     CDS             3284..3769
                     /locus_tag="VFB89_01545"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:3419..3421,aa:Sec)
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HZM26015.1"
                     /translation="MGQRAPDFSLTAATREGVKQRPVALHDFKGKTVVLAFFYKARTK
                     GUTIQMNAYRDQYATLFKGGKDVVLLAISADSAPALASWAKDANFPFTFLSDPLGDAG
                     KPYGAWQPDIKLDNRSVFVIGPDGKISYKATPFREVDPTAYEELGKAVATAAPTRTSP
                     Y"
     gene            complement(3797..>4829)
                     /gene="thrC"
                     /locus_tag="VFB89_01550"
     CDS             complement(3797..>4829)
                     /gene="thrC"
                     /locus_tag="VFB89_01550"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZM26016.1"
                     /translation="PEGNTPLLHRAALDRWTGLARLLLKHEGHNPTGSFKDRGMTVGV
                     TQAKRIGATAVACASTGNTSASLAAYAAQAGLPALVLVPAGNVALGKLAQSLAYGAKT
                     LLVRGDFDDCLRLVEQAGEQLGVYLLNSINPFRIEGQKTIVLEALEQLSWTPPDWIVL
                     PAGNLGNTAAFGKALVEARTWGLIDRLPRLAAVQAEGAAPFAQSFREGFARRHRVKAE
                     TVATAIRIGDPASYDRAVKAIRETNGVVLAVSDTEILEAKAAVDATGVGCEPASAASV
                     AGVRRLVAAGTIGSTESVVAVLTGHVLKDPGALLAYHQEAEPPPPGANRPVEIDPSLD
                     AVARILGSR"
BASE COUNT          822 a         1548 c         1557 g          902 t
ORIGIN      
        1 cgtacgcggt cttgttgccg tccagcgatt ccctgctctg gtccacatag gcgagctgga
       61 cggtcttgcc gacaaccagg tcgcccgcgt caggctgttc ctggccggtg atgatgcgga
      121 agagcgtcgt ctttccagcg ccgttgggac cgatcacgcc gacgataccg ccgcgaggga
      181 gtgaaaagtt gaggccttcg aagagcagct ggtcgccgaa gcccttcgcg acgttcttgg
      241 cgatcacgac ttcgtcgccc agccgctcgg ggacgggaat caggatttcg ctgctcgctt
      301 cggtttggcg ttccgcctcg gcgcgaagct cctcgtagcg tgtgatgcgg gccttgctct
      361 tcgcctgccg ggcgcgggga gccatgcgga tccactcgag ctcatgggct agggtcttct
      421 ggcgagccga tgcttgcttc tcttcgccgg cgagccgctg ctgcttttgc gccagccagg
      481 acgaatagtt tccctcccag ggaatgccgg cgccctggta aagctccagg atccacctgg
      541 ccacgttgtc gaggaagtaa cggtcatgag tgacggcgac tacggtcccg ggaaactcgg
      601 ccaggtaccg ctccagccac cagacgctct cagcatcgag gtgattggta ggttcatcga
      661 ggagaagcat gtccggctgt gcgagcagca cttggcagag cgcgacccgg cgccgctcgc
      721 cgcccgagat cttgccaacg tcggcctcgg ccggaggcag ccgcagtgcg tccatggcga
      781 ggtcgatctt gtgatcgagc tcccagaggc cgagcgcgtc gatccgctcc tgaagattgc
      841 cttgcttctc caacagacgg ttcatctcgt cgtcgtccat cggctcggcg aagcgggcgc
      901 tcagctgttc gaactggtcg agcaggtcgc gctgctcggc cacggcgact tcgacattgc
      961 cccgcacgtc cttggccggg tcgagccgcg gctcctgagg tagatagccg atcttgatgc
     1021 cagggagcgg tcgcgcgtca ccgagataat ccgtgtcctc gcccgccatg atgcgcagca
     1081 gggtcgactt gcccgcaccg ttggcgccca gcactccgat cttggcgccg gggtagaacg
     1141 cgaggtagat gcctttcagg atctctttat tggggggcac gaccttgcgg aggtcgcgca
     1201 tcgtgaagat gtattcgccg gccatggggc cgaagataaa cagcgtgaac ggtaaacagt
     1261 aaacggtgaa cgggaggtcg ctgagacagg acgcccccgt tgaccgctca ccgtttaccg
     1321 ttcacgctgt aaaacagtaa gggcgccccg acgggagcgc cctcgacact gcgccgcgtg
     1381 atgacctagc tcaggtgttt gttgaccagc ttggtcatct cgaacatgtt gacgacgtcc
     1441 ttcttgaaga tcggacgaag cgtctcgtcc gcgtggatgt tccgcctgtt gttcttgtcc
     1501 tgcagaccct tccgcttgat gtacgcccag agcttcttgg tgacttcggt ccggggtagc
     1561 gcgcccgagc cgacgacggc tgcgagctgc tcgctgggac gcatggcctt catgaatgcg
     1621 ggattgggct tccgctttgc tgccgtcttc ttcttggcgg gccggcgtct ggcggtagat
     1681 ctcttggcct tcttggacga cttcttcttg gctgccatcg cgagtctccc tgaggatggg
     1741 gggaaccgag cgtgcctccg aaggaccgaa tccgacgccc atcaaagtat gacgcttttc
     1801 tcccacggca atagggtgcg ccgctctctg tgccgctcgg tgtagccgag aacccggaga
     1861 gggagccata ttgatccatg actccgcctg tctcgaccct catgcgtgcc cgcgtgctgt
     1921 tgccagcggt cggagcgctc gcaatcttcg gtgtcttcct gaggcgccca cgactcgcgc
     1981 cgcctcgtca ctccgaacgg ccggtctgtg cgcccgacaa tggcgggctg aacgtgccga
     2041 acggcttctg cgcggtcgtt gtcgcgcacg atgtcggttc cgtgcggcac ctggtggtcg
     2101 ctcccaacgg agacatcttc gccaatcggg agcgcggtgg ggtgatcgcg ctgcgcgaca
     2161 cgtcgggcga cggagttgcc gatatagtcc gaccgttcaa cacgcagggt ggcaccggga
     2221 tcgccctcga gggcgacttc ctgtactact cgacggatgc cgccgtctat cgctatccct
     2281 ggaagagcgg acagctcgag ccgaagggcg aggccgagtc gatcgttatc gatctgccgg
     2341 cggacggtga ccataaatcc aagagcattg ccctcgggtc cgggggcgca ttgttcgtca
     2401 acatcggctc ggccaccaac agttgccagc agcagaaccg ggcgtcccgc tccccggggc
     2461 gggatccctg cgatgagctc aagacccggg cgggcatctg gcggttcgat gcgaatcgca
     2521 aggatcagcg tctacccgac ggcgagcact gggccacagg tctccgaaac accgtggcgc
     2581 tggcgaccga ccccaagagc cgtgcgctgt atggcgtgac ccacgggcgg gaccagttgc
     2641 gagagagctg ggggttctcg gccgagaagg gcgcggaaaa tccgggcgaa gagatggttc
     2701 gcccggagcg cggtgatgac tacggctggc cttactgcta ctacgacacc gacctgaagc
     2761 agaaagtgct ggcccccgaa tatggcggag acggcaagca ggtgggccgc tgtgcgcgaa
     2821 tgaaacccgc cctcgtttcc ttcccgggac attgggcgcc catggctctc gcctttgggg
     2881 atggcaagtt cggccctgcg tacgcgagcg gcgtcttcgt cgcgtttcac gggtcatgga
     2941 atcgggcgcc gctgccccag gcagggtacc gcgtcgtctg gcagccgatg gccgatggcc
     3001 ggccctcggg cacctatcaa agctttgtca ccggaaccaa gggaccgacc tcgatccgcc
     3061 ctagcggtct cgccattgga ccggatggat cgctgtacat ctctgccgat cagaatggaa
     3121 ccatctggcg cgtattgaac tcttccgacg ccgaacacac taaggaggga acgtgaaagt
     3181 ggcaggatgg gccgcggctg tcgtctgtgc cgccggtgcc gtcgcaactc cggctgcagg
     3241 gcaggcgggg cagggaacgt cacagcagga gcagggccct gccgtgggcc agcgagcccc
     3301 agatttctca ctcaccgcgg cgactcgcga aggagtaaag cagcgcccgg tagcgttgca
     3361 tgacttcaag gggaagaccg tggtgcttgc cttcttttac aaggcgagga ccaaaggctg
     3421 aaccatccag atgaacgcgt accgtgatca gtacgcgacg ctgttcaagg gtgggaaaga
     3481 tgttgttctg ctcgcgatca gcgcggactc ggcacccgcg ttggcttcct gggccaaaga
     3541 cgccaacttc ccattcacat tcctgtccga tccgctcggg gacgccggta agccttacgg
     3601 ggcctggcaa cccgacatca aactggacaa tcgcagcgtc ttcgtgattg gccctgacgg
     3661 aaagatctct tacaaggcga caccgttccg cgaagtggat ccgacggcgt acgaggagct
     3721 gggaaaggcc gtcgccacgg cagcgccgac ccgaacttcc ccctactgac aaacgagagc
     3781 cgctgaggcg cctgcctcag cggctcccca gaattcgcgc aacggcatcg agcgatgggt
     3841 cgatctccac cggtcggttg gccccgggcg gaggtggttc cgcctcctga tgatatgcta
     3901 gcagggcgcc gggatctttg agcacgtgcc cggtcagcac ggcgacgacg ctttcggtcg
     3961 agccgatggt tccggccgcg accagtcgcc gcacgcccgc cacgctcgca gcgctggcag
     4021 gctcacaccc tactcctgtc gcgtccacgg cggccttggc ctccagaatc tctgtatcgc
     4081 tcaccgcgag cacgacgccg ttggtttccc ggatggcttt gaccgcacga tcgtaggatg
     4141 cagggtcgcc gattcggatc gcagtcgcta ccgtttccgc cttcacccgg tgccggcgcg
     4201 caaagccctc gcggaaactc tgggcaaagg gcgccgctcc ctccgcctgc acagcagcca
     4261 atcgcggcag ccggtcgatc agcccccagg tcctggcctc caccagcgct tttccgaacg
     4321 ccgcggtgtt gcccagattg ccggcgggaa gcacgatcca gtcgggcggg gtccagctca
     4381 gctgttcgag cgcctcgagc acgattgtct tttgcccctc gatccgaaat ggattgatcg
     4441 aattgagcaa atagacgccg agctgttctc cggcctgctc gaccagtctc agacaatcat
     4501 cgaagtcgcc acgtacgagc agtgtcttcg ctccgtaggc gagcgactgg gccagctttc
     4561 caagggcgac gttgccggcc ggtaccagca cgagggcagg caagcctgcc tgcgccgcat
     4621 acgcagccag cgaagcagaa gtgttgcccg tggacgcgca cgcgaccgcg gtcgccccga
     4681 tgcgcttcgc ctgggtcacc cccacggtca tcccgcggtc cttgaatgag cccgtggggt
     4741 tgtgtccctc gtgcttgagc aggagccgcg ccagaccggt ccagcgatcc agcgccgcgc
     4801 ggtgaagcag cggggtgttc ccttcggga
//
LOCUS       DASRJQ010000067         5198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3902323, whole genome shotgun sequence.
ACCESSION   DASRJQ010000067 DASRJQ010000000
VERSION     DASRJQ010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3902323"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VFB89_01555"
     CDS             complement(<1..635)
                     /locus_tag="VFB89_01555"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZM26017.1"
                     /translation="METRPSAEGTAPGVPGPSPLERLDRALAGRYVVQRELGRGGMAT
                     VYLADDLKHQRKVAIKVLRPELGTLLGPDRFTREIRIAAALNHPHVVALYDSGDADAL
                     LFYVMPYVRGESVRQKLVRQGQLPIDEAIGIVRHVAAALDHAHAQGLIHRDIKPENIL
                     IHEGEAMVTDFGIALVAGAAPAERLTATGLMVGTPAYMSPEQAAGEHALDAR"
     gene            complement(726..1145)
                     /locus_tag="VFB89_01560"
     CDS             complement(726..1145)
                     /locus_tag="VFB89_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HZM26018.1"
                     /translation="MPADVPAADRAAFEGIVRQLETAWNAMDGSAFAVPFADDADFVN
                     IRGEHFRGRAAIAAGHVAIFRSIYAGSTNRLTLETARLLRADVALVHVRSVLQAPHGP
                     LAGRHSARFSLVLTREQGGWEIAAFHNTLEPAQKPPG"
     gene            complement(1189..2040)
                     /locus_tag="VFB89_01565"
     CDS             complement(1189..2040)
                     /locus_tag="VFB89_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HZM26019.1"
                     /translation="MDIQLVLVPYDTARRGWRSGLGPEHLVQAGLATYLRDRGHSVAD
                     IQVLEPKPDEPPAEIATGFELMRRIAVATREARRAGRFPIVLSGNCNSAVGTLSGLTP
                     ARRAIFWFDAHGDCNTPETTTSGFLDGTGLATALGLCWRELTATVPGYEPVQPEATFL
                     LAVRDLDPAEASLVERSAITTVSTAQIPAGVPEILARAPLDGALGYVHLDLDVLDPVG
                     VGRANSLPVPGGLSVEQLRASIAAIRTRVPLGAAGLASYAPEYDAGQAVCRAAFAALD
                     AILADGA"
     gene            complement(2080..2595)
                     /locus_tag="VFB89_01570"
     CDS             complement(2080..2595)
                     /locus_tag="VFB89_01570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZM26020.1"
                     /translation="MPQTVGTGPTVRPADAADSAAISRIYNYYILSTVATFEEEAVTA
                     EEIGRRIDAIRSAELPWLVAGDPTAGLVGYAYAAPWKPRSAYRYSVEITVYLAPECSG
                     RGLGTLLYDVLFARLKERSIHAVVGGIALPNPASVALHEKFGLQQVALFKEQGFKFGR
                     WVDVGYWQRLL"
     gene            2844..3068
                     /locus_tag="VFB89_01575"
     CDS             2844..3068
                     /locus_tag="VFB89_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26021.1"
                     /translation="MSEGRHSIEIPLTPEQQALIHRLSGQHAEILELTPEPQDPATGV
                     GQTLRFRWRLSDATGIPRLNWVRRKPTSGG"
     gene            complement(3191..3559)
                     /locus_tag="VFB89_01580"
     CDS             complement(3191..3559)
                     /locus_tag="VFB89_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26022.1"
                     /translation="MTLAERGLYHQIHPAKLLIDWTAGLVAAGLLWQHHLASALVVGL
                     LPPVCASAILISDYFEPALQHYRTSAFGRYVARHMTRGMQGLRLVGLVLIWWGSWHQR
                     LAVAALGVIVVLTAWIRGLW"
     gene            complement(3777..>5198)
                     /locus_tag="VFB89_01585"
     CDS             complement(3777..>5198)
                     /locus_tag="VFB89_01585"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26023.1"
                     /translation="RDSVGDGARQVVREARVAVENDSAQAIMARWSAQLGPDSSDRRM
                     LLGLATAARLSYDDTTATRLYQRLLGSPGSGDAYGVYAQLGLARIFFDRADLRAAESA
                     ASTALLQARALHDRSTEGEALLAVADARMDEDAKVGRAYLDSALKALPTAATEPIAEA
                     QCRRVRLGFRSGDARFPRELPVALAYARRVGATRAEAQCLRTAAIDLWTSGQLDSATV
                     LLRHSAQLQGTVRDRRSLAFTLATLADLLRDQGAYGEARTTVAAALEQARASHYVWGE
                     ATATHLTGTLAYSLRDLSTAASALDRADTLYGTLGDSADQMNVRSWQANIARDRGDLT
                     TARRVTREVIAAARREQAVPWTVDLFQALADIEIIAGDYPAAAAALDTAGQILRKQGT
                     RSWEAKLVYQRGRLALHQGDLDSAERIFRAYLRTLDNDEGLRRHEIQGYLADIRVRRG
                     DLTGAERDLAAAGERATGHRCPT"
BASE COUNT          918 a         1729 c         1713 g          838 t
ORIGIN      
        1 cgggcatcca gggcgtgctc gccggcggcc tgctcgggac tcatgtacgc aggcgtcccg
       61 accatgagtc cggtcgcggt cagccgctca gctggtgctg caccggccac cagcgcgatc
      121 ccgaagtcgg tgaccatcgc ttcgccttcg tggatcagaa tgttttcggg cttgatgtcg
      181 cggtggatca gcccctgcgc gtgagcatga tccagggccg ccgcgacgtg tcgcacgatg
      241 ccgatcgcct cgtcaatcgg gagctggccc tgccgcacca gcttctggcg aaccgattcg
      301 ccccgaacgt agggcatgac gtagaacagc aacgcatcgg catcccccga gtcgtaaagc
      361 gcaacgacgt ggggatggtt gagcgctgcg gcaatcctga tctcgcgggt gaaccgatcg
      421 ggtccgagca gtgtacccag ctcggggcgg aggaccttga tggccacctt ccgctggtgc
      481 ttcaggtcat ccgccagata gacggtggcc atgccgcccc ggccgagctc gcgctggacg
      541 acatagcggc cggcgagggc gcggtcgaga cgctcaagcg gggacggccc cgggactccc
      601 ggagccgtcc cttcggctga cggcctagtc tccaccgtag cgcacctcga tcgcgatcat
      661 cgctgcctcg ctcatgccaa tcgggtctgc aaacttacgg cgccccgcat gcccacggaa
      721 ccatttcatc cgggcggttt ctgcgccggt tcgagcgtgt tatgaaatgc ggcgatctcc
      781 caaccacctt gctccctggt cagaaccagg gaaaagcggg ccgagtgccg cccagcaagc
      841 ggaccgtgcg gagcctgcag tacggagcgc acatgtacca gggcaacgtc cgctctgagt
      901 aagcgggccg tctcaagggt gagccggttg gtgctcccgg cgtaaatgct ccggaagatt
      961 gctacgtggc cggcggcgat cgccgcgcgc ccgcggaaat gctcgccccg gatgtttaca
     1021 aagtcggcat cgtcggcgaa gggtacggcg aacgccgagc catccatcgc gttccaggct
     1081 gtctcgagct ggcgaacgat gccctcgaag gcggcacgat cggcggctgg cacgtcagca
     1141 ggcatcgctc tcctcgggta cgcggaccga tttattgacc aggtgagttc aggcgccgtc
     1201 ggcaagaatg gcatcgagtg ccgcgaaggc cgcgcggcag accgcctgcc cagcatcgta
     1261 ctcgggcgcg tacgaggcaa gtccggcggc tccgagcggg acgcgggtac ggatggctgc
     1321 aatgctcgct ctgagctgct cgaccgagag cccgcccggc accggcaacg agttggcgcg
     1381 gcccacgcca accgggtcca gcacgtcgag gtcgaggtga acgtagccca gggcaccgtc
     1441 cagcggcgcc ctggccagga tttccggaac gccggcgggg atctgagcgg tcgataccgt
     1501 agtgattgcc gatcgttcaa ccagcgacgc ctcggctggg tcgaggtcgc gcaccgccag
     1561 caggaaggtg gcctcgggtt gcacaggctc gtacccgggc actgtcgcgg tgagctcgcg
     1621 ccagcacaag ccaagtgccg tggcgagtcc agtgccatcg aggaaaccgg acgtggtggt
     1681 ctcaggggtg ttgcagtccc cgtgggcatc gaaccagaaa atcgcccggc gcgctggcgt
     1741 caggccgctc aaggtgccga cagctgagtt gcaattcccg ctcaagacta tgggaaaccg
     1801 tccggcgcgg cgggcttcac gggtcgccac ggcgatgcgc cgcatgagct caaacccggt
     1861 ggcgatctcc gccggtggct catccggctt gggctcgagc acctgaatgt cggcgacaga
     1921 atggccgcgg tcacgcagat aggtcgcaag tcctgcctgc actagatgct cggggccgag
     1981 gccactgcgc catccacgcc gcgcggtatc gtagggaacg agaacgagct gaatatccat
     2041 atggctgaat actccggaag tggcgcagag agcgaggcgc tacaatagcc gttgccagta
     2101 cccgacgtcc acccagcggc cgaatttgaa accctgctcc ttaaacagcg ccacctgctg
     2161 cagtccaaat ttctcgtgca acgccacgct cgccggattc ggcagggcga tgccgcccac
     2221 gacggcgtga atactccgct ctttcaacct ggcgaacagg acatcgtaca gcagagtgcc
     2281 aagtccgcga ccgctgcact ccggggccag gtacacggta atttcaacgg aatatcggta
     2341 ggccgaacgg ggcttccagg gcgccgcgta ggcgtagccg accaggccgg cagttgggtc
     2401 gccggcaacg agccacggca actcggcgct ccgaatcgcg tcgatgcgcc gaccgatctc
     2461 ctccgccgtg accgcttctt cttcaaaggt ggcgacggtg ctgagaatgt agtagttgta
     2521 gatgcgggag atagcggccg agtccgcagc atctgccggc cgcacggttg gtcctgtccc
     2581 aacggtttgg ggcatgtctt cgtaacctta attcgaggtc gcgaggcggt gggtttggcg
     2641 gccactgacg ccaagttgcc aacagcggtt agaccgcgag tccggatcac aacagtgtaa
     2701 cgatcagttc acgaggctcg agggacgctt gccgcaaacc ttcccggccg atcactttaa
     2761 ctctccttct gcaaccgacc gctgttgcgg atccgcatga gcaatcctat ccgagaacga
     2821 cccgagcact tatcggagct ggtatgagtg aagggcgaca ttccatcgag attccgctga
     2881 ccccagagca gcaggctctc atccaccggc tctccggcca gcacgctgaa attctggagc
     2941 tgaccccgga gccacaggac ccggcgactg gcgttggtca aacgctacgc ttccgctggc
     3001 gactctcgga cgcgaccgga attccccgcc tgaactgggt gcggcggaag cccacatccg
     3061 gcggctgaac ttcccaagcg cccctttcca gccccccgag ggtgcgcagt cagtgatgtt
     3121 cagtcgagcg gtctcgcacg caggcgaatg cgcttggccg tcgcgacatg gatgttcgcg
     3181 atggtggcgt ctaccacaaa ccgcgaatcc atgcggtgag cactacgatc acgcccagcg
     3241 ctgcgacagc aaggcgctga tgccagctgc cccaccagat gagcacgagg ccgacgagcc
     3301 ttagtccctg catgccacgg gtcatatgcc gggcgacgta gcggccgaag gcagacgtcc
     3361 gataatgctg caaggccggc tcgaaatagt cgctgatcaa aatggcggac gcgcaaaccg
     3421 gcggtagcaa gccgacgacc aacgccgaag ccagatgatg ctgccagagc aggccggcgg
     3481 cgacgagccc tgcggtccag tcgatcagga gcttcgccgg gtggatctgg tggtagagcc
     3541 ctcgctcggc gagcgtcacg gtatcctagg catcccctga agcgcgcccc cgcgatgcac
     3601 gcgataggcc gagcgcggcg acgattgcaa taatgaccag gccgccggcg ccagcccacc
     3661 acaccgttcg ggaccgcggc gccgtaagcg gcacgacggt cgcggggtca ccgaccaatt
     3721 ggaacgccgc ccagatgcgt ggcgggagtc cctcccggtt cgccgccagc ttggcttcac
     3781 gtagggcatc gatgaccggt cgccctttcg ccggcagcgg cgaggtcgcg ctcggcaccg
     3841 gtgagatcac cgcggcgaac ccggatgtct gcgagatagc cctggatctc gtgccggcgc
     3901 agcccttcat cgttgtccag cgtgcggagg taggcgcgga agatccgctc cgccgagtcg
     3961 aggtcaccct gatgcagcgc caggcggccg cgttgataca ccagctttgc ctcccacgac
     4021 ctggtaccct gcttccggag aatctgcccc gcggtgtcga gcgcggccgc agcggcgggg
     4081 taatctccgg cgattatctc gatatcggcg agggcctgaa atagatcgac cgtccacggc
     4141 accgcctgct ctcgccgcgc cgcggcgatg acctcgcggg tcacccgccg tgctgtcgtc
     4201 aggtcgccgc gatcgcgcgc gatgttcgcc tgccaggagc gcacgttcat ctggtcggcc
     4261 gaatccccca gcgtgccgta caaagtgtcc gcccggtcca aggcactcgc ggctgtggag
     4321 agatcgcgca gcgaatacgc cagtgtgccg gtgagatggg tggcggtcgc ctcgccccag
     4381 acgtagtgtg acgctcgcgc ctgttcgagc gctgctgcca cagtcgttct cgcctcgcca
     4441 taggcgccct ggtcgcggag caggtccgcc aacgtcgcca gagtaaaggc tagagaccgc
     4501 cggtctcgaa cagtgccttg gagctgagcg gagtgccgca gcagtaccgt ggctgagtcg
     4561 agctgcccgc tggtccagag gtcgatcgcc gcggtacgca ggcactgcgc ttccgcacgc
     4621 gtggccccaa cccgccgggc gtacgccagc gcgaccggaa gctctcgcgg gaatcgggcg
     4681 tcgccgctcc ggaaccccag gcggacgcgc cgacattgcg cctccgcaat aggttctgtc
     4741 gccgcggtcg gcagcgcctt gagagcgctg tcgagatagg ctcgaccgac cttcgcgtcc
     4801 tcgtccatac gcgcgtcggc cacagccagc agcgcttcac cttcggtgct tcggtcatgg
     4861 agggcgcggg cctgcaggag tgccgtactg gcggcgctct cggccgcccg caagtcggcc
     4921 cgatcaaaga aaatccgcgc aaggccgagt tgcgcgtaga cgccgtaggc gtcgccgctg
     4981 cccggcgaac cgagcagccg ctgatagagg cgcgtcgccg tcgtgtcgtc gtaggacagt
     5041 cgggccgcag tcgccaaacc gagaagcatg cggcggtcgg aggaatcggg acccagttgt
     5101 gccgaccagc gtgccatgat ggcctgagca ctgtcgttct cgacggcgac tcgcgcttca
     5161 cgcacgacct gccgggcgcc gtcgccgacg ctgtcgcg
//
LOCUS       DASRJQ010000068         7608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1087424, whole genome shotgun sequence.
ACCESSION   DASRJQ010000068 DASRJQ010000000
VERSION     DASRJQ010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7608
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087424"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..553)
                     /gene="bstA"
                     /locus_tag="VFB89_01590"
     CDS             complement(29..553)
                     /gene="bstA"
                     /locus_tag="VFB89_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol transferase BstA"
                     /protein_id="HZM26024.1"
                     /translation="MTDLRYPIGKFQPPASVTHAERAVLVDQIAAIPAELRSVVSTLD
                     DTQLDTPYREGGWTVRQVVHHVPDSHVNAYTRFKLALTEDVPTIKTYEEAQWAELPEA
                     RSAPIGVSLALLEALHQRWVLLLRVLAPAQFARTFRHPELGIMTLDQQIALYAWHGRH
                     HLAHITSLRTRMGW"
     gene            complement(619..2355)
                     /locus_tag="VFB89_01595"
     CDS             complement(619..2355)
                     /locus_tag="VFB89_01595"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM26025.1"
                     /translation="MTDNQPQSPLGMSPFPGAEREELEALRAATLGHYDVLGELGRGG
                     MATVYLAHDIALDRKVAIKVMSPMLSLGDGVERFRREARTSASLSHPNIIPIYTVRHS
                     DRLLYFVMKFVEGRSLDSIIAELGQLPVPMVQAILGQAASAFGYAHRRGVVHRDIKPG
                     NILIDEEGWAVITDFGIAKVAQAPQLTSTGLSVGTPTYMSPEQVEGKGVTGASDQYSL
                     GVVAYEMLTGRPPFIGGGMMSTMFAHVHEDPEPLEQLRPDCPEGLRSAVMRMLAKDVG
                     DRWPSIEDIIAVIGSPSLKPDDPTRSQLIAIAKTGQRGSINVATPKSPIPLGRTPSPI
                     RVPTGGADAGMVAGSAAGATSEAPVQPRRASVMSLALAAGGILVGVGSLVVALRGHSP
                     APDRTQETGASTVTAAAVPPLPTGAPAAPAPPTAPPNGSVPSERPKGEPPAKPAEPGA
                     TATATPPPGEPSSGSAAERNAIESTIRSYASALDSGSVAAARGVFPAMPDSQQAALES
                     FFSGGGRITTLWRVSDVNLDGDRATARIKGASRLEPPDSTPAVRPVNLRAVLERTPDG
                     WQLRSLGGSGAP"
     gene            complement(2834..3091)
                     /locus_tag="VFB89_01600"
     CDS             complement(2834..3091)
                     /locus_tag="VFB89_01600"
                     /inference="COORDINATES: protein motif:HMM:NF025715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4404 family protein"
                     /protein_id="HZM26026.1"
                     /translation="MTRERLKQLLEQLHAELAGTDAVDPGVRERLEGIQRDVGEALKR
                     ESPPGTLRDRLENEVAQFEASHPTVARRMAEVIDTLALYGL"
     gene            complement(3105..4451)
                     /locus_tag="VFB89_01605"
     CDS             complement(3105..4451)
                     /locus_tag="VFB89_01605"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZM26027.1"
                     /translation="MSTLLLALTSLLLPSAAPHQGPTPPAPGDSTVVAFDSVTVVPMD
                     RERVLDSQTVIVRDGKIAEIGPSNSVQVPAGAIRVNGKGKYLMPGIAEMHAHVPSRDD
                     PTLDAVMALYVLTGATTVRAMMGTPDQLEYRKEIASGLMLGPTLFAVGPPFNGDNTKN
                     PQDAEAKVRQFHAAGFDVLKIFPGMNRETYDAILRTARELHMPISGHVPSSVGVRHAI
                     ESGQSIEHLDGYLEDIGRDRGRIQELVQLTTTAGIWNCPTMDVWKTILGLRDPDELLR
                     DRPEVQYMPPQLVDQWTKRVREMGRKSPVRLALEGLGMSRSAADIAALRDTLLRALSD
                     GGAKLLLGSDSPQAFSVPGFSLAHEMDAMVAAGLSNWTVLSAATRNPAEFFGRSAEFG
                     TVEVGKRADLLLLDGNPLDDIRNVHRQAGVMVRGHWLAAEEINRRLAQIAAAWKRR"
     gene            4728..5225
                     /locus_tag="VFB89_01610"
     CDS             4728..5225
                     /locus_tag="VFB89_01610"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZM26028.1"
                     /translation="MFEAGEVKFVILRLLREKPRHGYEIIKDLEERMGGHYTPSAGTV
                     YPTLQLLEDQGYVRAVETEGRKVYHITPEGERFLDENRDVIDDIIDRVRDTLRDVAGG
                     HMGELNAAFARLAAVTYKRAWRRGPDHPSLQRVVEILRRAIEDIEREWEQPQTGGGPE
                     RSTAS"
     gene            5222..5506
                     /locus_tag="VFB89_01615"
     CDS             5222..5506
                     /locus_tag="VFB89_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26029.1"
                     /translation="MIWVHGTWWIPLLIFALIFWRPWRWGRRGYNYWQWPPPRHYQGP
                     DRAPELDEAVRARLDLVDQLETRVTELENRLDFAERLLAERKEEKPLGLG"
     gene            complement(5513..6949)
                     /locus_tag="VFB89_01620"
     CDS             complement(5513..6949)
                     /locus_tag="VFB89_01620"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HZM26030.1"
                     /translation="MTALQTAIAVGHVLLAIAVSVHIVLTKEDVRAAIGWTGLVWLTP
                     VIGSVMYGLFGINRIRRQAGMMRRGRQLADSRTAGLAATHGPDQLLPADTPSSLLGIA
                     TIGGRVTGLPLTPCNAVDLLVNGDEAYPAMMAAIDEAKRSVGLATYIFDRGVAGDGFV
                     AALGRAVARGVEVRVLIDGVGARYSRPPVFRALRERRVRVAEFLPPRIPLVQPYSQLR
                     NHRKLMVVDGQVGFCGGMNIRDDCLLALKLPSATQDVHFRLRGPVIQQLGQTFAFDWA
                     FTTKERLDGERWACDPQVAGNVVARGLPEGPDEDFETLMLVLLGALSQARRSIRLATP
                     YFLPDLPLVDALRTAALRGVLVDIVLPERGNLKMVEWAAMAQLGQVLKWGCQVYLSRQ
                     PFDHSKILVVDGNWSLVGSANWDPRSLRLNFEYNVECYSTELAAQLESLLDQKIASGR
                     RMTLADLEGRPLPLRLRDGIARLAQPYL"
     gene            complement(6955..7401)
                     /locus_tag="VFB89_01625"
     CDS             complement(6955..7401)
                     /locus_tag="VFB89_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26031.1"
                     /translation="MSRSRFGWTLLGAVIGSLACHQETGRLTPDQEQRLAAEGILHRA
                     DNLRFRWTEGAGRRGGTWEDRLASIVVTRRSVLIHKNAKVGVEITPGGARHYEVHRDG
                     DRVRISIGSGRSSETWSFAAPDSADAWTRDIRAVIRLSLPHGATPQ"
BASE COUNT         1365 a         2429 c         2479 g         1335 t
ORIGIN      
        1 gtaatcatcg taagctcggt gagagagtct accagcccat gcgagttcgc agcgatgtaa
       61 tgtgcgccag atgatgccgg ccgtgccagg cgtacagtgc aatctgctga tcgagcgtca
      121 taatccccag ctcaggatgc ctaaaggtgc gagcaaattg ggcgggcgcc aggacccgga
      181 gcaaaagcac ccagcgctga tgcagcgcct cgagcaaagc gagcgagacc ccaatcggcg
      241 ccgatcgcgc ttcgggcagc tcggcccact gggcttcctc gtacgtcttt atggttggta
      301 cgtcttccgt gagcgcgagc ttgaagcggg tataggcgtt cacgtggcta tcgggcacat
      361 ggtggaccac ctgccgcaca gtccagccgc cctcacggta gggtgtatct agctgagtgt
      421 cgtccagtgt gctgacgaca gaccggagct cggcagggat tgcagcgatc tgatcgacta
      481 gcactgctcg ttctgcatgg gtgacactgg caggaggctg gaatttgccg atgggatagc
      541 gaaggtcggt catgaggagc tccggcgctg aggcgaacgg gggacggtac ccctacgggc
      601 ccggagtcgc ggcggttttc agggcgcgcc agaccctccc aggctgcgaa gctgccaacc
      661 gtctggcgta cgctccaaca cggcgcgcag gtttaccggc cgcaccgccg gggtactgtc
      721 cggcggttcc aggcgactcg cgcctttgat ccgggcggtg gcgcggtcac cgtcgaggtt
      781 gacgtcgctc acccgccaga gcgtcgtgat gcggcccccg cccgagaaga acgactccag
      841 cgcggcctgt tgggaatcgg gcatggcggg gaacacacct cgcgcagcag cgacgctgcc
      901 ggaatcgagc gcggaggcgt acgatcggat cgtcgactcg atcgcgttgc gctcagcggc
      961 ggatccactg gaaggctcac caggcggggg cgtcgccgtg gcggttgccc ctggttcggc
     1021 gggcttggcc gggggctcgc ccttcgggcg ttcagatgga acgctcccgt tcggcggcgc
     1081 agtgggcggt gccggcgcgg ctggcgcgcc ggtcgggaga ggcgggacgg cggcagccgt
     1141 gactgtcgat gcgccggtct cctgcgttcg atcgggggcc ggagaatgcc ctcgcagggc
     1201 aaccaccaag gagcccacgc cgaccaggat gccgcctgct gcaagcgcga gcgacatgac
     1261 agaggcgcgg cgcggctgga cgggagcctc gctggtcgct cctgctgccg agccggcgac
     1321 catgcctgcg tccgcgccgc cggtgggcac ccggattggg ctaggcgttc gtcccagtgg
     1381 aatcggactc ttgggcgtcg cgacgttgat gctgccccgc tgcccggtct tcgcaattgc
     1441 gatcaactgg ctgcgggtcg gatcgtcggg cttgagggat ggcgacccga tcaccgcgat
     1501 aatgtcttcg atcgagggcc agcggtcgcc gacgtccttg gcgagcatcc gcatgactgc
     1561 gctccgaagc ccttcggggc aatcggggcg cagctgctcc agcggctcgg gatcctcgtg
     1621 cacgtgggcg aacatcgtcg acatcatccc gccacctatg aagggcggcc tcccggtcag
     1681 catttcgtag gcaacgacgc caagcgaata ctgatccgat gcaccggtga ctcccttccc
     1741 ttccacctgc tccggactca tgtaggtcgg agttccgacc gagagtccag tgctggtcaa
     1801 ctgcggtgcc tgggccacct tcgcgatgcc gaagtcggtg atgacggccc agccctcttc
     1861 atcgatcagg atgttgccgg gcttgatgtc gcgatggacc acgccccgcc ggtgggcgta
     1921 gccgaaggcg ctggccgcct gtcccaggat cgcttgcacc atcgggactg gaagttgacc
     1981 caattcagcg atgatcgaat cgagcgaacg gccctcgacg aacttcatca cgaagtagag
     2041 gagccgatcg ctgtgacgga ccgtgtagat ggggatgatg ttggggtggc tgagcgacgc
     2101 tgacgtgcgg gcttcgcgcc taaagcgttc tactccgtcg cccaggctca gcatgggaga
     2161 catcaccttg atcgcgactt ttcgatcgag ggcgatgtca tgggccaggt aaaccgtcgc
     2221 catccctccc cgacccagct cgccgagcac gtcgtagtgg ccgagggtgg ccgcgcgaag
     2281 tgcctccagc tcttcccgtt cggcgccggg gaatgggctc atgcctagag gagactgggg
     2341 ctggttatcg gtcatggtct cagcgcgatt ctgagcttcc ggcccggcag ccggcctgct
     2401 tgacggtcca tcgtcaacat gacggaaaca gcaagtggtt gcaatgccgg ggccggttgt
     2461 ctgggcattt aatacgccga cggccgccca ccgaattggc ctgatgccgg cggcgtcgcc
     2521 aggcgtgggc cgccgccgct tcccgcttgc cgagaccgtt cagcggggtc ggcacttacc
     2581 cctaaccttt gccaactgag gtgcttacac acagcacctg gcgggcgaac cttacgacac
     2641 tcagcacttc aacccgagaa ggcgaaaggt tcgggtcgct cagcgtgggt cagccagctg
     2701 actgcgatgg ggaccgtcga gagaagttcg ccgagcgggt gcagcagcgg tgatgcgtgc
     2761 ttgccgcgac cggtgcagat ccttgcgcga tgctggtgcc aagcagattg gaaatctcga
     2821 tcgatgcagc ggcctagagg ccgtagagcg ccaatgtgtc gatcacctcg gccattcgcc
     2881 gagccacagt gggatgcgat gcttcgaact gcgcgacctc gttctccaga cggtcgcgca
     2941 gtgtgcccgg cggactctcg cgcttcaatg cctcgcccac gtcccgctgt atgccttcca
     3001 gccgttcgcg cacacctgga tcgaccgcat cggtaccggc gagctcggca tgaagctgct
     3061 ccaggagctg tttcagtcgc tcacgcgtca tctccgctcc cccattatcg gcgtttccat
     3121 gccgcagcaa tctgcgccag tcgccggttg atttcctccg ccgccaacca atgccctcgc
     3181 accatcaccc ccgcctggcg gtgaacgttc cgaatgtcgt cgagcggatt gccgtcaagc
     3241 agcaggagat ccgcacgctt gccgacctcc acggtaccga actccgcgga tcgaccaaaa
     3301 aactcggccg ggttacgagt cgctgcggac agaaccgtcc agttcgacag gcccgcggca
     3361 accatggcgt ccatctcgtg agccagtgaa aacccgggca cgctgaaggc ctggggcgag
     3421 tccgatccca ggagcagctt ggctccgccg tccgacagcg cccgcaggag cgtgtcgcga
     3481 agcgcagcga tgtcggccgc tgatcgactc atccccaacc cctcgagcgc gaggcgcacc
     3541 ggactcttgc gccccatctc gcgcactcgc ttggtccact gatcgaccag ctgcggcggc
     3601 atatactgga cttcgggtcg gtcgcggagc agctcatccg gatcgcgcaa accgaggatc
     3661 gtcttccaga cgtccatggt ggggcagttc cagatcccgg ccgtcgtcgt cagttgaacc
     3721 agctcctgta tccgtccgcg atcccgcccg atgtcctcga ggtagccgtc cagatgctcg
     3781 atggattgcc cggactcgat ggcgtgccgc acgcccactg acgacggcac gtgcccggag
     3841 atgggcatgt ggagctcgcg agctgtcctc agtatcgcat cgtatgtctc gcgattcatt
     3901 ccggggaaga tcttgagcac gtcgaaaccg gccgcatgga actggcggac cttggcctcg
     3961 gcgtcctgcg ggttcttggt gttgtcccca ttgaagggtg gcccaaccgc gaacaacgtc
     4021 ggaccgagca tcaggccgga cgcaatctcc ttccgatatt cgagctggtc gggcgtgccc
     4081 atcatggccc ggaccgtggt cgcccccgtg agcacataga gggccatcac tgcatccagg
     4141 gtggggtcgt cgcgggaggg aacgtgggca tgcatttcgg cgattccggg catcaggtac
     4201 ttccctttgc cattcacccg aatcgcacca gccggcacct gaactgagtt gctcgggccg
     4261 atctcggcaa tcttgccgtc gcgcacgatg accgtctgac tgtccagaac gcgctctcga
     4321 tccatcggga ccaccgtgac cgagtcgaag gccacgactg tggagtcgcc gggcgcaggc
     4381 ggcgtgggcc cctgatgcgg agcggccgac gggagcagca gggaggtaag tgccaggagc
     4441 aaagtggaca tggaggctga gctgaacgga ggtcagtgaa gagcgatccg gaattatggg
     4501 ttgaaccggt ctgcaccgcc atccgaaact ccgcgggtcc gcttgacgta tcgtccgtta
     4561 agtcgtacga tatatcgcgc gttatgacga atcagccagc gaggtgctaa gtgggtaact
     4621 gtcgagatga ctggggtttg ggcgacttgt tcgggggctt cggccccgga ggcttcggct
     4681 tccagtggag acaccagggt cgcggcgggc gccaccgccg agggcccatg ttcgaggccg
     4741 gagaggtcaa gttcgtgatt cttcggctgc ttcgtgaaaa gcctcgccac ggctacgaaa
     4801 tcatcaagga cctggaagag cgtatgggcg gccattacac cccgtccgcc ggaacggtgt
     4861 atccaacgct tcagctgctg gaagaccagg gctacgtccg cgccgtagag accgagggtc
     4921 ggaaggttta ccacatcacg cccgaaggcg agcgctttct ggacgaaaac cgcgacgtca
     4981 tcgatgacat catcgatcgg gtacgtgaca cgctccgcga tgtcgccggc ggtcacatgg
     5041 gcgagttgaa cgcagcgttc gcgcgcctgg ccgcggtgac gtacaaacgg gcctggcgcc
     5101 gtggtccgga tcatccttcg cttcagcgtg tggtcgagat actccgtcga gctattgagg
     5161 acatcgaacg ggaatgggag cagccgcaaa cgggcggagg cccggagagg agcaccgcat
     5221 catgatctgg gtacatggga cgtggtggat cccgttgctc atcttcgcgc tcattttctg
     5281 gcggccctgg cgttggggcc ggcgcgggta caattactgg cagtggccgc cccctcggca
     5341 ctatcaaggg cccgaccgag cgccggagct ggacgaagcg gtgcgagctc ggctcgacct
     5401 ggtggaccag ctcgagaccc gcgtgaccga gttggagaat cggttggatt tcgcggaacg
     5461 actactggcc gagcggaagg aagaaaagcc gttgggccta ggctagcggc acctacaaat
     5521 acggctgcgc caatcgcgcg atcccatcgc gcaagcgaag cggcagcgga cgaccctcga
     5581 gatcggccag ggtcatccgt ctgccgctcg cgattttctg atccagaaga ctttccagct
     5641 gagccgccag ctcagtcgaa tagcattcca cgttgtactc gaaattgagt cgtaagctcc
     5701 ggggatccca gtttgccgac ccgacgagcg accagttccc atctaccacc aggatcttgg
     5761 aatggtcgaa cggctgacgg gagagataga cctggcaacc ccacttgagc acctgcccca
     5821 actgcgccat tgccgcccac tcaaccatct tgaggttgcc tcgttcaggc agaacgatat
     5881 ccactagcac gcctctcagc gcggcggttc gcagcgcgtc gaccagcggc aaatcgggga
     5941 ggaagtaggg cgtggccaac cggatcgacc ggcgtgcctg ggagagggca ccgaggagaa
     6001 cgagcatcaa cgtctcgaag tcttcatccg gtccttcggg aagtccgcgg gcgaccacgt
     6061 tcccggcaac ctgcggatcg caagcccacc gctcgccatc gagacgttcc ttggtggtaa
     6121 atgcccaatc gaacgcgaat gtctggccga gctgctgaat caccggtcca cgcagccgaa
     6181 aatgcacgtc ctgcgttgcg ctcggcagct tgagagccag cagacaatcg tcgcggatat
     6241 tcatcccgcc gcagaacccg acctgcccat ccaccaccat cagcttgcga tgattgcgga
     6301 gttgggagta cggctgcacc agcggaatgc gcggaggcag gaattcggcg acgcgcaccc
     6361 gtcgctcgcg taaggctcga aacaccgggg gtcggctgta gcgagcgccg accccgtcta
     6421 tcagaacccg gacctcgacc cctcgcgcca cggctcgtcc gagcgccgca acgaagccat
     6481 caccggcaac gccccggtcg aagatgtagg tggcgagtcc aacgctccgc ttggcctcgt
     6541 cgattgccgc catcatcgcc gggtaggcct cgtcgccatt caccagcagg tccacggcat
     6601 tgcagggggt caggggtagg cccgttacac ggccgccaat cgtggcaatg cccagaaggc
     6661 tcgacggggt atcggcaggc agcagctgat cgggtccgtg agtcgccgcc agccccgcag
     6721 ttcggctgtc ggcgagttgt cggcctcgcc gcatcattcc cgcctggcgg cgaatccgat
     6781 tgatcccgaa gagaccatac atcacggaac cgataacagg cgtcagccag accagaccgg
     6841 tccagccgat cgccgcgcgc acatcttcct tcgtaagcac gatgtgcacc gagacggcga
     6901 tggcgaggag aacgtgtccg acagcgatcg ccgtttggag cgccgtcatc ccgttcactg
     6961 cggggtggct ccgtggggca aggagaggcg gatgactgcc cggatatcgc gggtccatgc
     7021 gtcggcgctg tccggggcgg cgaaggacca cgtttccgac gacctgccgc tgccgatgct
     7081 gatccgcacg cgatcgccgt ctcgatgcac ctcgtaatgg cgcgcgcccc cgggcgtgat
     7141 ttcgacgccc accttggcgt tcttgtggat cagaaccgaa cggcgagtca cgacgatgga
     7201 ggcaagccga tcttcccagg ttccgccacg gcgacccgcc ccctcggtcc agcggaaccg
     7261 taggttgtcg gcgcggtgga gtatgccctc cgcggcgaga cgctgttcct ggtccggggt
     7321 gaggcgaccg gtctcctggt gacatgccag gctgccgatg acagcgccga gcaaggtcca
     7381 gccgaatcgg gatcgggaca cgcaacctcg catggatcgt agacttgaat gaccgcgacg
     7441 gttggagctt ctggtgcatc ccaaaggcgc gaccgggccg gactttcctg cccgcagttc
     7501 gccccaatgt cgccgcggac ccgatttacc gtacggtccg cctgtcacta acgccagacg
     7561 agccagcatg tcttttctca ccctcgatgc agtcacgaag cggttcga
//
LOCUS       DASRJQ010000069         5354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2203733, whole genome shotgun sequence.
ACCESSION   DASRJQ010000069 DASRJQ010000000
VERSION     DASRJQ010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2203733"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="VFB89_01630"
     CDS             complement(<1..866)
                     /locus_tag="VFB89_01630"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26032.1"
                     /translation="MNLLVGALSVGLLLSLLTLGVFLTYRVLNRLDLTTDGSFGLGAA
                     VAAMLLANGVHPVTATVIATIAGWLAGAATGVVHTAFGVDTLLGGILVSTALYSVQLY
                     VMHGGDVSLAAADTLPGLAERMWTALGGPAQVTLFGTSVSAGNIAALVLFVVFVLGSM
                     FLMERFLRTDLGLAVRATGDNPQMARALGVDVGFMVTLGLVMANGLIALSGALFAQYQ
                     GFANVQMGLGIVVVGLACLIVGEALVGRSGPARQVTGVLIGTIVFRLLVAAALRAGLD
                     ANALKLVTAVFVL"
     gene            complement(961..1971)
                     /locus_tag="VFB89_01635"
     CDS             complement(961..1971)
                     /locus_tag="VFB89_01635"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZM26033.1"
                     /translation="MTPQRGSVWQSFVRPTALAAALGIALACNKDTSADKAPPATNSA
                     APASAPAIVALIRAADWIGSEWSEDALKVGLQEEGMEQDRDFVFKSSSAQGDLATLPN
                     LVDAALDQKAKVIVTLQDATLQVAVKRVKETPIVFHILSDPFAAGAGTSDSVHLPNVT
                     GVYSPGFGDPEQERRIQLIKQLVPKAKRVGVLFSPGEQLSVDLKNRLTSAAKKGGLQV
                     EAVPMNTPGDATEAINLLVGKKVDAIEIYGNAAHAGFESIIKVAKEKKIPVFSPSPFE
                     IVKGATAALYPDFQEGGVVAGKMIARILRGESPAKIPFYRLQTTKTQLADSSKGGAAK
                     PQ"
     gene            complement(2091..2945)
                     /locus_tag="VFB89_01640"
     CDS             complement(2091..2945)
                     /locus_tag="VFB89_01640"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZM26034.1"
                     /translation="MAQPETVGTHFEATFRTADGLELFRQQWRPVVSPRAILVNVHGI
                     GDHSGLYPMLPDYFVPRGLAVHAFDMRGNGRSPGPRGHIDSWTQLRDDLQRFVAVVRA
                     QEDGRPMFLLGNSLGGLVVLDYALHHPAGLRGVIAIAPPLGRIGTPAWLLTLGRIMSH
                     IWPGFTLETGLDLSRLSRDPAACEAIVTDPLFHRKGSARLAAEVLRTAHGLHERAGDF
                     PVPLLLLHGAEDRMVFPDGTREFFGVVGQPDKRYVEYPGAFHALLADLDRDRVLSELE
                     SWMDKRRA"
     gene            3034..3561
                     /locus_tag="VFB89_01645"
     CDS             3034..3561
                     /locus_tag="VFB89_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZM26035.1"
                     /translation="MTLRHEMVAALMLTATMTLSGAVPMQAQFGKRLTQTVQSSAENR
                     AIQEVVNQENKAIDAALSSGLSSGDKSAGDTLYATLKSDGRAPVTGIGFKEKTATMTD
                     QSATSLKAIGAMLEAHSDLKLRIEANADDKNVAVARAQAVRDALVKAYGIDAGRLQAE
                     GYKKAGDQSVDLVQL"
     gene            complement(3558..4118)
                     /locus_tag="VFB89_01650"
     CDS             complement(3558..4118)
                     /locus_tag="VFB89_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26036.1"
                     /translation="MRRPPVERLPFTAVMCYFLYVASPLTLSEIRAMLPAGITADLLP
                     PADQRKLRELHPAARTGVRLLIGRCSCDLLISRDGEAREGERHLRNRFFAAGFDRRQV
                     IAGLEAHRSGARPRPAPPSEWQAALAEFVAEHARNAGPTAYLLRFGSDSLQAHEPAER
                     ATLTTADVRGQPAAWLVEDRLTTVVR"
     gene            complement(4164..4532)
                     /locus_tag="VFB89_01655"
     CDS             complement(4164..4532)
                     /locus_tag="VFB89_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES family protein"
                     /protein_id="HZM26037.1"
                     /translation="MEFPKKQLLVVGDRVLITPEEGEDRTRVGLYLPPTALDTQQVQT
                     GLIVATGPGDPVADLGSLDDEPWKLEASDRRSRAMQAKVGDHAIFFRKAAVEITFEDK
                     KYLVVPQAAILVLVRDDLQI"
     gene            complement(4607..5083)
                     /locus_tag="VFB89_01660"
     CDS             complement(4607..5083)
                     /locus_tag="VFB89_01660"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HZM26038.1"
                     /translation="MRDKLSREVDQLSHELNVLLPQAIAQAVALGDLRENSEYKSALE
                     RQQFVQARLGQLHQRLNQLTQLANAEAPTDRVGLGSRVTVLDLETNETDVYTVVLAEM
                     MDIDAGHISLASPLGRALKDGKVGDEISLRLPTRTRKVRITELVTIHQTESVNGKR"
BASE COUNT         1040 a         1693 c         1723 g          898 t
ORIGIN      
        1 agcacgaaca ccgcggtcac cagcttgagt gcgtttgcat cgagaccggc gcggagcgcg
       61 gccgcaacca ggagacggaa aacgatggtg ccgatgagaa caccggtgac ctggcgcgcc
      121 ggcccgctcc gccccaccag cgcttcgcct acgatgagac aggcgagccc caccaccacg
      181 atcccgaggc ccatctgtac gttcgcgaac ccttggtact gcgcgaacag cgccccggaa
      241 agggcgatca gaccgttggc catgaccagg ccaagcgtca ccatgaaacc gacgtccact
      301 ccgagcgccc gggccatctg agggttgtcg ccggtggcac gcaccgcgag tcccagatcg
      361 gtgcgaagaa accgctccat caggaacatg ctgcccagca cgaacacgac gaagagaacc
      421 agtgcagcga tgttaccggc actcacagat gtgccgaaga gcgtcacttg cgcggggccg
      481 ccgagggccg tccacatccg ctcggccaat ccgggcagcg tatctgcagc ggcgagtgag
      541 acatccccgc cgtgcatgac gtaaagctga accgaataga gcgccgtgct tacgagaata
      601 ccgccgagca acgtgtcgac gccgaacgcc gtgtgcacca cgccggtcgc ggcgccggcc
      661 agccagccgg cgatggtcgc gattacggtg gccgtgaccg gatggacacc gttggccagc
      721 agcatcgccg ccacggctgc cccaagtcca aaagatccgt cggtggtgag atcgagccgg
      781 ttgagcacgc ggtaggtgag aaacaccccg agcgtgagca acgacaggag cagcccaact
      841 gaaagggcac cgaccagcag gttcatgtgg cgcgaggagc gggtccggcg cccgcgatgc
      901 agtgcctacg ggcggcgctg cgcacgacag caaggccgcc gtcgtgcgcg ctcgaaatcc
      961 tcactgaggc ttcgcagcgc ctcccttgga gctgtcggcc agttgggtct tggtcgtctg
     1021 gagacggtag aacgggatct tggccggact ctcacctctg aggatgcggg caatcatctt
     1081 acccgcgaca acgccgcctt cctggaaatc cggatagaga gccgccgtcg cgcccttcac
     1141 gatctcgaag ggtgagggag agaaaaccgg gatcttcttt tcctttgcga ctttgatgat
     1201 gctctcgaac ccggcgtggg ccgcatttcc gtagatctcg atagcgtcga ccttctttcc
     1261 gacgagcaga ttgattgctt ccgtcgcgtc accgggcgtg ttcatgggaa ccgcttccac
     1321 ctgtagaccg cccttcttgg cggcgctggt gagccggttc ttcaagtcga ccgacagctg
     1381 ctcgccggga ctgaacagca cgccgactcg cttggccttg gggacgagct gcttgatcag
     1441 ctgaatccgc cgctcctgtt cgggatcgcc gaagccgggt gaatagacgc cggtgacatt
     1501 cggcagatgg acggagtcgc tggtgccggc tccggcggcg aacggatccg acaggatgtg
     1561 gaagacgatc ggcgtctcct tgacccgctt gaccgcaacc tggagggtag cgtcctgcag
     1621 cgtcacgatg acctttgcct tctggtcgag ggccgcgtcg accaggttcg gcaacgtggc
     1681 cagatcgccc tgagcgctgg aggacttgaa gacgaagtct cgatcctgct ccatcccctc
     1741 ctcctgcaag cccaccttga gggcatcctc gctccactca ctgccgatcc agtcggctgc
     1801 ccggatcagt gctacgatcg caggcgccga ggcgggcgcg gccgagttgg tggcgggcgg
     1861 cgccttatcg gcggaggtgt ccttgttgca ggcgagggcg atcccgaggg ccgcggcgag
     1921 cgccgtgggc cggacgaagg actgccaaac ggacccacgc tgcggggtca tgcgactgcc
     1981 tcccaggtga cggtaaacat ctcgaggcga agcgtgtcat tttattcacg gctcagggtt
     2041 tccgcaaaga acgctacagg atcgtcggct ggccgacgac ctggtcgttc ctaggcgcgc
     2101 cgcttgtcca tccagctctc caattcggaa agcacgcgat cccgatcgag atcggccagc
     2161 agggcgtgaa atgcccctgg gtactcgacg tatcgcttat cgggctgccc gactacaccg
     2221 aagaattccc gagtgccgtc ggggaacacc attcgatcct cggctccatg gagcaggagg
     2281 agaggcaccg ggaagtcgcc ggcgcgctca tgcaggccat gcgcagttct gagaacttcg
     2341 gccgcaaggc gagcagagcc tttgcggtgg aacaaggggt cggtcacgat cgcctcgcac
     2401 gccgccgggt ctcgagacag tcgggagaga tctaggccgg tttcgagggt gaagccgggc
     2461 cagatatgcg acatgattcg ccccagggtg aggagccagg ccggcgtccc gatccggcct
     2521 agcggcggcg cgatggcaat gacgccgcgc aatccagctg gatggtgcag ggcatagtcg
     2581 agcaccacca acccgccgag gctgttgccc agaaggaaca tcggccggcc gtcctcctgg
     2641 gctcggacaa cggcgacgaa tcgctgaagg tcgtctcgca gctgagtcca gctgtcgatg
     2701 tggccccggg gaccggggga gcggccattg ccacgcatat cgaacgcgtg aaccgccagt
     2761 ccgcggggaa cgaagtagtc aggcagcatg gggtacaggc cgctgtggtc gccgattcca
     2821 tgcacgttga ccagtatggc gcggggggag acgaccggac gccactgctg acggaacagt
     2881 tcgagcccgt cagcggtccg aaaggtcgct tcgaagtggg ttccaaccgt ctcgggttgc
     2941 gccatcgttg gagcctcgct tgacaccgcc ggtgcgggga tcaaacatag ccgcaggcag
     3001 gcagtccccc gtcccatccc ggagaccgat gtcatgaccc tcagacacga gatggtcgcg
     3061 gctctgatgc tgaccgcgac gatgaccttg agcggtgccg tgccgatgca agcccagttc
     3121 gggaagcgac tcacgcagac ggtacagagc agtgcggaaa accgcgctat ccaggaggta
     3181 gtgaaccagg agaacaaggc gatcgatgcc gctctgtcgt ccggcctctc ttccggggac
     3241 aagtcggcgg gcgacacgct ctacgcgacg ctcaagtccg acggccgggc tccggtgacc
     3301 gggatcggct tcaaggagaa gacggcaacg atgaccgacc agtcggcgac ctcactcaag
     3361 gccataggcg ccatgctcga agctcacagc gatctcaagc ttcggatcga ggccaatgcc
     3421 gacgacaaga acgtcgcagt agcgcgtgcg caggctgtcc gggatgctct ggtgaaggcc
     3481 tacggaatcg acgccgggcg gttgcaagcc gagggataca agaaagcggg agaccagtcg
     3541 gtcgatctgg tgcagctcta gcgaactacg gttgtcaggc gatcctcgac cagccacgcg
     3601 gccggctgac cgcgcacgtc tgcggtggta agcgtggcgc gctcggcagg ctcatgggcc
     3661 tgcaaggagt cagagccgaa gcgcagcagg taggccgtcg gtccggcatt ccgagcgtgc
     3721 tcggccacga actcggccag cgctgcttgc cactcgctgg gcggggcagg gcggggccgt
     3781 gcacctgatc gatgggcctc gagcccggcg attacctgtc gccgatcgaa gccagccgcg
     3841 aaaaaccggt tccgcaggtg ccgctcccct tcacgagcct ccccatcacg agagatcagc
     3901 agatcgcagg aacaacggcc gatcaacaga cggacgccgg ttcgagccgc gggatgaagc
     3961 tcgcgcagtt tccgctgatc agccggcgga agcaggtctg ccgtgatgcc ggcaggaagc
     4021 atcgcccgga tttcggagag ggtgaggggg ctcgcgacgt agaggaagta gcacatgact
     4081 gcggtgaacg gtaaacgctc aacggggggc cgccgcaatc ggggacctcc cgttcaccgt
     4141 tcgccgtgca ctgtttacgc tattcaaatc tgcaaatcgt ctcgcaccaa caccagaatc
     4201 gcggcctgcg ggacaaccag atacttcttg tcctcgaagg tgatctcgac ggcggccttc
     4261 cggaagaaaa tcgcgtgatc cccgaccttt gcctgcatcg cgcgggaacg tcggtcactc
     4321 gcttccaact tccagggttc gtcgtccagg ctaccgagat ccgctaccgg atcgcccggg
     4381 ccggtggcga cgatcagccc ggtctgtacc tgctgggtat ccagcgccgt cggcgggagg
     4441 tacagcccga cccgggtgcg atcctcgcct tcctcaggcg tgatgagcac cctgtcgccg
     4501 acgacgagga gttgcttctt cgggaattcc atgagtcaac agcgtaaacg gtgaaccgaa
     4561 aacaatgaac gggggcttcg ttgcattccg agcccccgtt caccgttcag cgcttcccgt
     4621 tcacgctttc ggtctggtgt atggtgacca gctccgtgat ccggaccttt cgcgtcctgg
     4681 tcgggagacg aaggctgatc tcgtctccga cctttccgtc cttgagcgcc cgcccaagcg
     4741 gcgaggcgag cgaaatgtga ccggcatcaa tgtccatcat ttctgccagc acaaccgtat
     4801 acacatcagt ctcattcgtc tcgaggtcca agaccgtgac gcgcgagccc aggccaaccc
     4861 gatcagtagg cgcctcggcg ttggccagtt gagtcaactg gttgagccgt tggtgcaact
     4921 ggcccaacct ggcctgcaca aactgctggc gctcgagcgc cgacttgtac tccgagttct
     4981 cccgcaagtc acccagcgcc accgcctgtg cgatagcttg aggaaggagt acgttgagct
     5041 catggctgag ctggtcgact tctcggctca gcttgtcccg catttcattg atcatggttg
     5101 ggccagaaag acaagaaaac aaagccccgg aaaagccaat ctctccgggt cagtccaggg
     5161 cagattccac cggaatgata ctacacctcg gctgggagcc gatagggtga cctcaactgc
     5221 aagaggtgca atcagttggg tgatcctagc tctcggtctc gggttgccgg cgccggcgga
     5281 agcccaggcc ggcaccgact cgtcgtcagc tgcctatcac gccatccaat ggtacgagcc
     5341 gctggcagtg atgg
//
LOCUS       DASRJQ010000070         2019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1925429, whole genome shotgun sequence.
ACCESSION   DASRJQ010000070 DASRJQ010000000
VERSION     DASRJQ010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1925429"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VFB89_01665"
     CDS             complement(<1..350)
                     /locus_tag="VFB89_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26039.1"
                     /translation="MNLSITVEQPDKKLALFGSADRYLRMIRDTFGVQLVSRDDEIKI
                     SGDGEQVSRAAAVLEQMQKKLRRQDWLSVEDVGQAIGKAADQQRERSADEIDVYAKGH
                     AVKPKTAGQKRYMDA"
     gene            621..1490
                     /locus_tag="VFB89_01670"
     CDS             621..1490
                     /locus_tag="VFB89_01670"
                     /inference="COORDINATES: protein motif:HMM:NF019231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HZM26040.1"
                     /translation="MGKRGRAFTLIELICVIGIIGILIALLMPSLIRVRESARSLKCL
                     SNLRQLGIAFHSYAAEQNGYLPYPTTLLYPPPTDQRFLWFNAVDSYMRANSKEQEKRT
                     GIAATRNYKDYKQCVVYENFEGDRDEGDQSSTKEYARTIKMNAHLRRANPPNHAKVTD
                     VNDSSNFVMLGDGVSLDSTGMVPELWESGQFSMEVNDTTHANPAPRHHRAANILFVDG
                     HAAAVQLKRIGKSLRDPYEFVSVATWESEYLDATGTPVPAKGDQTIEEQGLHRNPNMP
                     LRWSDPPRLYRPP"
     gene            complement(1570..>2019)
                     /locus_tag="VFB89_01675"
     CDS             complement(1570..>2019)
                     /locus_tag="VFB89_01675"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HZM26041.1"
                     /translation="FLMALRVKHSDYPNGFHGTTWTILSILLMVKFTDIGAYFGGRAF
                     GRRKLIYWLSPGKTWEGLFFGLMTAGIVGALCAPGLPHVPWWKGFIFGVIIGGIGQLG
                     DLLESLMKRDAEVKDSGRLVPGFGGILDIIDSPLVAAPFAYLLFSLF"
BASE COUNT          403 a          654 c          612 g          350 t
ORIGIN      
        1 gcgtccatat agcgtttttg accggccgtc ttgggcttga cggcgtggcc cttggcgtaa
       61 acgtcgatct cgtccgcact tcgctcacgt tgctgatccg ccgctttgcc gatcgcctgg
      121 ccgacgtctt cgacgctgag ccaatcctgc cggcggagct tcttctgcat ctgttccagt
      181 accgcggcgg ccctggaaac ctgctcgccg tcgccggaga ttttgatttc atcatcccgg
      241 ctgacgagct gaacgccgaa ggtgtcgcgg atcatgcgca gatagcggtc cgccgacccg
      301 aagagggcga gcttcttatc tggttgttcg acggtgatgc taaggttcac aatagaaacg
      361 cgcggtgcgt cgcggtgctt acgtccgccc gaggcgggct gttccctcca ccggtccgat
      421 attgttgcgg cggaggggct aaatgtcgag attcaatcgt ccccccgcca aggcgggctg
      481 cgaataccgg ctaactatag gtagcggcga gcgttaaagc actttttccc gggagaatgt
      541 ccgggggtgg gcggctcagg caattacccg gtgcaaggca tgggcggcat agccgtctta
      601 gacagaccgg gagggtctac atggggaaac ggggccgggc gttcaccctc atcgagctga
      661 tctgcgtgat cgggatcatc gggatcctca tcgcgctgct gatgccgtcc ctgatccgcg
      721 tccgcgagtc ggcccggtcg ctgaagtgcc tgagcaacct tcgccagctc ggcatcgcct
      781 tccattccta cgcggcggag cagaacggct acctgccgta tccgacgacg ctgctctacc
      841 cgccgccgac cgatcagcgc ttcctctggt tcaacgccgt cgactcctac atgcgggcca
      901 actctaagga acaggagaag cgcacgggca tcgccgcgac ccggaactac aaggattaca
      961 agcagtgcgt cgtctacgaa aacttcgaag gcgaccggga cgagggcgac cagagcagca
     1021 cgaaggaata cgcccgcacg atcaagatga acgcccacct ccgccgcgcc aatccgccca
     1081 atcatgcgaa ggtgaccgat gtgaacgact cgtcgaactt cgtcatgctc ggcgacggcg
     1141 tgtcgctgga ctcgaccggc atggtgcctg agctctggga gagcgggcag ttttcgatgg
     1201 aggtcaacga cacgacgcac gcgaaccccg cgccgcgcca tcaccgggcg gcgaacatcc
     1261 ttttcgtcga cggccacgcc gccgcggtcc agctcaagcg catcggcaaa tcgctgcgcg
     1321 acccgtacga gttcgtgagc gtcgcgacgt gggagagcga gtacttggac gcgaccggca
     1381 ccccggtgcc ggcgaagggg gaccagacga tcgaggagca ggggttgcat cgcaacccga
     1441 acatgccgct gagatggagc gatccaccga ggttgtatcg acccccgtag ggtgctacgc
     1501 ggtggaaatg catcccgggt tcccggcgat gctgtcgcaa gcgcagcgtc cccgcgggat
     1561 ccgttcgcat cagaaaagac taaacagcag atacgcgaac ggcgccgcaa ccagcggact
     1621 gtcgatgatg tcgaggattc ccccgaaccc cggcaccaac cgtcccgaat ccttcacttc
     1681 cgcatctcgc ttcatcaaac tctcgagcaa atccccgagc tgcccaatcc cgccgatgat
     1741 gacgccgaag atgaaaccct tccaccacgg gacgtgcggc aaacccggcg cgcacagcgc
     1801 acccacgatg ccggcggtca tgagcccgaa gaacaacccc tcccacgtct tcccgggact
     1861 gagccagtag atcaacttcc gtcgcccgaa cgcccggccg ccgaagtagg cgccgatgtc
     1921 ggtgaatttc accatcagca ggatcgaaag gatcgtccag gtcgtaccgt ggaagccgtt
     1981 gggatagtcg gaatgcttca cgcgcaaggc catcagaaa
//
LOCUS       DASRJQ010000071         3084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_4183246, whole genome shotgun sequence.
ACCESSION   DASRJQ010000071 DASRJQ010000000
VERSION     DASRJQ010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4183246"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1591)
                     /locus_tag="VFB89_01680"
     CDS             complement(<1..1591)
                     /locus_tag="VFB89_01680"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR03121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit A"
                     /protein_id="HZM26042.1"
                     /translation="MPTGGRLRIVGGAVHDPANNIDGEIRDVCIEDGRIVASLPGGAP
                     TLNANGMIVMPGGVDIHAHFASSSCNHARRLLPEEHTADPVPAPKLLDGAGRSGTGGT
                     VPTTFSTAYRYAGLGYTTAFDAAVAPIMARHTHAELDDTPILDAGCFVLLGNDEYLHR
                     QISRGERERAAHYVAWMLAATGAYAVKIVNPGGIEAWKSGQRNLTDIDQALAGTNVTP
                     RAILETLTNAANGLRLPHPVHIHCNNLGQAGNSATTIATMEALTGRRAHFTHLQFHCY
                     GGEAGKGWSSAAKSVIEHVNAHPEHTVDVGQVMFGPATTLTADGPVEYLLYSTSGRKW
                     VNIDVELETGCGIVPYSYKEKAAVATLQWAVGLELFLLARDPWRVVLSTDHPNGGSFL
                     SYPALIQLLMTRGARDEQLQRANPKLLAGSALTDGLDREYTLNEIAIVTRAGPARILG
                     LRHKGHLGVGADADITIYGRDTDIAKMFSSPRFVLKAGQLVVEEGQLRRAPQGRRLHV
                     RPPYDESILRDVRKHFDDYGTV"
     gene            complement(1591..2841)
                     /locus_tag="VFB89_01685"
     CDS             complement(1591..2841)
                     /locus_tag="VFB89_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26043.1"
                     /translation="MTEGTVSHAVCLGCGCACDDIEVTVRNGRITGAINACPIGVEWY
                     GDGTVPGKIMVRGAQATMEKATRAVAELLRAGPALIYLAPDVTTETQRAAIELADRCG
                     ARLDSVTSDTAAPGILVGQRRGRATATLGEIRNRADLLVFWAVDPATRYPRLLARYAV
                     DPSGTQVPKGASDRRVVAVDVGGATGPRTASTRIALSDANEAAALGVMRAAVLGRELP
                     GLSSELQAAVDLARQMTKARYVALIHDSELEGQGGDSQRLEGLVGLAQTLNGPTRCAL
                     SSLRAGGNRSGADAAMVWQTGFPFAVDFAAGAPRYRPEQPASRAIDGVGGALIVGAAH
                     RAPEVVTRALSGKRIAVIGPRATQVADAEVAVDTGIAGIHDAGIGYRMDDVPLPLPSI
                     LAGPASAADVIRTVATAVATGAKS"
     gene            complement(2838..>3084)
                     /locus_tag="VFB89_01690"
     CDS             complement(2838..>3084)
                     /locus_tag="VFB89_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26044.1"
                     /translation="LSHVSGKGIVYARETITVRPSSIWQSGTVADIPQEGERIAAGRP
                     VCTVFARGRSARDCRRALAVAANSVYHATGRIRRGVA"
BASE COUNT          511 a         1022 c          990 g          561 t
ORIGIN      
        1 ccacggtccc gtaatcgtca aagtgcttac gtacgtcgcg cagaatgctt tcgtcgtagg
       61 gaggccgaac gtggagccgg cgtccttgtg gcgcgcgtcg gagttgaccc tcttcgacaa
      121 cgagctgccc tgccttcagg acgaagcgtg ggctcgagaa catcttcgcg atgtcggtat
      181 cgcgcccgta gatcgtaatg tccgcgtcgg cccccacgcc caggtgcccc ttgtgtctga
      241 ggccgagaat ccgcgctggt cctgcccggg taacgatcgc gatttcgttg agggtgtact
      301 cacggtccag tccatcggtc agggcacttc cggctagcag cttcggattc gcccgctgga
      361 gttgctcatc ccgcgccccg cgggtcatga gcagctggat caacgcaggg taagagagga
      421 aagacccgcc gttggggtga tcggtcgaca ggaccactcg ccacgggtcg cgagcgagca
      481 ggaacaactc gaggccgacg gcccattgca gcgtggcgac agcagccttc tccttgtacg
      541 agtagggcac gatgccgcat ccggtctcca actcgacatc gatgttcacc cacttgcggc
      601 cgctggtgga gtagagcagg tactcgacgg ggccgtctgc cgtgagcgta gtcgcagggc
      661 cgaacatcac ctggcccacg tcgacggtat gctcgggatg ggcattgacg tgctcgatca
      721 cgctcttcgc cgctgagctc cagcccttgc ctgcttcgcc accgtagcag tggaattgca
      781 ggtgggtgaa gtgagccctc cgaccggtca gcgcctccat ggtcgcgatg gtagtggcgc
      841 tgttcccagc ctgccccagg ttgttgcagt ggatgtggac ggggtgcgga agccgcaaac
      901 cgttggcggc attggtcagc gtttccagga tggcccgggg tgtcacgttg gtccccgcca
      961 acgcctgatc aatgtcggtc aggttgcgct gccccgattt ccaggcctcg atgccacccg
     1021 ggttcacgat cttgaccgcg taggcaccgg tcgccgccag catccacgcg acatagtggg
     1081 cagcccgctc tcgttcaccg cggctgatct gccgatggag atactcgtca ttacccagga
     1141 gcacgaaaca ccctgcgtcc aggatcgggg tatcgtcgag ctccgcgtgg gtgtgccgcg
     1201 ccatgatggg tgcaactgct gcgtcgaacg ccgtggtgta cccgagtcca gcgtagcggt
     1261 aggcggtgct gaaggtcgtc ggcaccgtcc cgcctgttcc cgaccgaccc gcgccatcga
     1321 ggagttttgg cgcgggaacc gggtcagcag tgtgctcttc cggaagcaag cgtcgcgcgt
     1381 gattgcagga cgagctggca aaatgggcgt ggatgtccac gccccccggc atcacgatca
     1441 tgccgttcgc attcagtgtg ggcgcgccac cgggcaggct cgcgacgatc cgaccgtcct
     1501 cgatgcacac atcgcgaatt tcaccgtcga tgttgttggc aggatcgtgg actgcaccac
     1561 cgacgattcg gagtcgcccc cctgttggca tcaggatttt gctccggtcg ccaccgcggt
     1621 tgcgacagtg cggatgacgt cggccgccga cgccggccca gccagtatgc tcggcaatgg
     1681 caacggcaca tcatccatcc ggtagccgat tccggcgtca tgaatgccag cgatcccggt
     1741 atcaacggcc acttccgcgt cggcaacctg ggtggcgcgt gggccgatga cggcaattcg
     1801 ctttccgctg agcgcgcggg ttactacttc cggagcccga tgagcagctc cgacgataag
     1861 cgcgccgcca acgccgtcta tcgctcggga tgctggctgc tcagggcgat atcgcggggc
     1921 accggccgcg aagtcgacgg caaacgggaa tccggtctgc cataccatgg cggcatcggc
     1981 gccggaccgg ttcccgcccg ccctgaggct cgacagcgcg cagcgggtcg gtccgttgag
     2041 cgtctgagcg agcccaacta acccttccaa tctctgagaa tcgccgcctt ggccttccaa
     2101 ctcggagtca tggatcaggg cgacgtaacg agccttcgtc atctggcggg cgaggtccac
     2161 tgcggcctga agctcggaag acaaccccgg aagctcgcgg cccagcaccg cggcccgcat
     2221 cacgcccagg gctgcggctt cgtttgcatc tgatagcgcg attcgggtgc tcgcggtccg
     2281 aggcccagtt gcgcccccaa cgtcaaccgc gacaacacgc cggtcggacg cgcctttcgg
     2341 aacctgcgtt ccggaggggt cgaccgcgta gcgtgcaaga aggcggggat accgggttgc
     2401 agggtccact gcccagaaca ccagcaggtc ggcgcgattg cggatctcgc ccaacgtcgc
     2461 tgttgctctg ccccgccgct gacccaccag aatgcccggt gctgcagtgt cggacgtgac
     2521 actgtcgagt cgcgcgccgc agcggtcggc aagctcgatc gcggcccgct gcgtttcggt
     2581 cgtgacatcg ggagcaaggt agatcagagc cgggccggct cggaggagtt cggccaccgc
     2641 gcgcgttgcc ttctccatcg ttgcttgagc tccacgtacc ataatcttgc ccggcacagt
     2701 gccgtcgccg taccactcga ctccgatcgg acatgcgttg atcgcgccgg tgatgcgtcc
     2761 gttgcgcacc gtgacttcga tgtcgtcaca ggcgcagccg cagcctaggc acacggcatg
     2821 actgactgtc ccctcagtca cgcgacacct cgccggattc tgccggttgc gtgatacacg
     2881 gagttggccg caaccgccaa cgctctcctg caatcccggg cggagcgccc tcgcgcgaat
     2941 acagtgcaga ccggccggcc ggcagcgatc cgctcgccct cctgaggaat gtcggctacc
     3001 gtaccgctct gccagatcga gctcggcctc acggtgatgg tctcgcgcgc gtacacgatc
     3061 cccttgccgg acacgtgcga gaga
//
LOCUS       DASRJQ010000072         4604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1357279, whole genome shotgun sequence.
ACCESSION   DASRJQ010000072 DASRJQ010000000
VERSION     DASRJQ010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4604
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357279"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VFB89_01695"
     CDS             complement(<1..685)
                     /locus_tag="VFB89_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26045.1"
                     /translation="MSVEIERLAQGSVPARLDDIGVRERPSMALARRLAILTAILALV
                     LVLGATEISLSWSERSRLEDFRDDAMALANTLSALLTDIAPTGNPDSLAQGLEDEALQ
                     RLGPSRKAWIYLATGSMLTLVASSDTVLDHPADDADALAFARRAMIVRRLDAPLSGWH
                     VATPLGRGRPFGVLDITVSTRKLEAWARVERQRAYLAAVISALLLALGVALLTRKWVG
                     RPLESLGQAM"
     gene            complement(688..1623)
                     /locus_tag="VFB89_01700"
     CDS             complement(688..1623)
                     /locus_tag="VFB89_01700"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HZM26046.1"
                     /translation="MKPWRWVLALLAVVTAVLAVQFAVRFPWAGTLTAVARADWLLLG
                     AAAIANIVSLGAKAWAWHLVLLPRARHRFRTAQAATFVAAAVNSVSVSVSGEAARLHL
                     VATRDAVPVNAGLWSLVWCRLIEALGLVVFLALILAIIPTESWMRPFEIGAWIVLGLL
                     GAAWSLGVGPKLLSSIPASWRSRITEWSTPPKRWLAPLGLSVVNWAAQWLTFHLAFVA
                     THVSTTANVSLSALVMANLGGVFRITPGNVGVLQVSLVLGMLAFHIAEDQALAAGLTL
                     QAVQVVPILAIGVALVGAQGLRSLGAKRTEAAEAA"
     gene            complement(1620..2318)
                     /locus_tag="VFB89_01705"
     CDS             complement(1620..2318)
                     /locus_tag="VFB89_01705"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZM26047.1"
                     /translation="MIGVAVAAGLAGAAGLLTYGALEPTNRLFGPVTSRGPRDRSVYL
                     TFDDGPNEGATPAILDTLAEAGVPATFFLVGRHVSRYPELTRRIAAAGHGIGNHTQTH
                     AKLHRLGPHRIERELRSAHQAIVEASGTIPTMFRAPHGYRNPFVFRAAKALGYRVMGW
                     SFGVWDTARPGAEEIRRRILARVGPGSILLLHDGDGYDPTGDRRQTAEALPGIIADVR
                     ARGYEFQPLAEIAT"
     gene            complement(2315..3448)
                     /locus_tag="VFB89_01710"
     CDS             complement(2315..3448)
                     /locus_tag="VFB89_01710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZM26048.1"
                     /translation="MRIALVTEFYYPHLGGVTEHVHNLAKVYNAAGHPAIVVTSNMRM
                     PTGTPDAHEYEKDEDFVRRVGTSRIIYSSGSFARITTGWRLRRQLRDLFRREQVDIVH
                     VHGGLAPTFGIIAPLAASDLGIPVVATFHSWFAKSLALRFWRSEAKWLLGHQAANIAV
                     SQPVVEAHARYVDADWEIIPNGVDTAFFQPNGRSPAAPEVQDPKLLFLGRLDPRNGLE
                     TVLQAMPAVLERFPRTRLTIAGDGPLRPLYERLARPVAEHVDFLGRVNGNRPEVYGSA
                     DLYLCPTTKASFGITLLEAMACGTPLVVSDITGFRELVARDSEAVLVNKDDATAWAQV
                     TIDLLADPRRRSAMRSAGLAKAQTFAWPRVAEQVFAVYQRVTK"
     gene            4313..>4604
                     /locus_tag="VFB89_01715"
     CDS             4313..>4604
                     /locus_tag="VFB89_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26049.1"
                     /translation="MLALLLLMRGLSAPWLMLAVLYSVSRPRSGDDTTSSDKFQDPQL
                     SLVVPRDSGVTVLVPKLATTLKEYTRQQFANDLVAGVIVGVVALPLAIAFAIA"
BASE COUNT          839 a         1487 c         1506 g          772 t
ORIGIN      
        1 ccattgcctg acccagcgac tcgagcggtc gccccaccca cttgcgcgtc agcagcgcga
       61 cccccagagc gagcagcaac gccgagatga cggcggctaa gtaagcgcgc tgccgctcga
      121 cccgcgccca cgcctcgagc ttcctggtcg agaccgttat gtccaggact ccgaacggtc
      181 tccctcgacc aagcggcgtg gctacgtgcc atcccgagag cggtgcgtcc agccgccgca
      241 cgatcatggc gcgccgggcg aaggcgagcg catcagcgtc gtcggcggga tgatcgagta
      301 cggtatcgct cgacgcgacc agggtgagca tcgagccggt ggccaggtaa atccaggcct
      361 tccgtgaggg accgagacgc tgcagggctt catcttcgag cccctgagcc agcgaatcgg
      421 ggttgccggt gggggcgata tcggtgagaa gtgcggagag cgtattcgcc agcgccatcg
      481 catcgtcccg aaaatcctcg agccgggagc gctcggacca ggaaagcgag atttcggtcg
      541 cacccagtac cagaaccaag gcgaggatcg ccgtcaggat cgccagccgc cgggccagcg
      601 ccatggaggg ccgttcccgc accccgatat catccagccg tgccggcacg gagccttgtg
      661 ccagccgctc gatctcgacg ctcatcgtca cgccgcctcg gctgcctcgg tccgcttcgc
      721 tcccagactc cgcaagcctt gagcccccac caacgcaacg ccgatcgcga ggataggaac
      781 gacctgcaca gcctgaagcg tgaggccggc cgcgagcgcc tggtcctccg cgatgtgaaa
      841 ggcgagcatg ccgagcacca gcgatacctg caagacgccg acgttaccgg gggtaatgcg
      901 gaaaactccg ccaaggttgg ccatcactag cgcggagagg gagacattgg ccgtggtgga
      961 cacgtgggtc gcgacgaacg cgaggtggaa cgtgagccac tgggctgccc agttgacgac
     1021 cgagagtccg aggggagcaa gccaacgctt cggcggtgtg ctccactccg tgatccgcga
     1081 ccgccacgac gcgggtatgg aggagagcaa cttgggaccc acaccgaggg accaggctgc
     1141 gccaagaagg cccagaacga tccaggcccc gatctcgaag gggcgcatcc acgactcggt
     1201 ggggatgatc gccaggatga gagcgagaaa gacaaccagc ccgagagctt cgatcagacg
     1261 gcaccagacc agcgaccaga gcccggcgtt caccggcacg gcatcgcgcg tggccaccag
     1321 gtgaagccgc gccgcttcac cgctgaccga cacgctgacc gagttgactg ccgcggcaac
     1381 aaacgtggcg gcctgggctg tgcgaaaccg atgccgtgcg cgcggaagca ggacgaggtg
     1441 ccaggcccaa gccttggcgc cgagcgatac gatgttcgcg atggcggcgg cgcccaacag
     1501 aagccagtca gccctcgcga ctgcggtcag ggtcccggcc cagggaaagc gaacagcgaa
     1561 ctggaccgcg agcaccgctg tgacgacggc gaggagcgcc agaacccaac gccaaggctt
     1621 catgtcgcga tctcggccaa cggctggaat tcgtacccac gggcgcgcac gtcggcgatg
     1681 ataccgggga gcgcctcggc ggtttgccgg cggtcacccg tgggatcgta cccgtctccg
     1741 tcgtggagca agagaatcga gcccgggccg acgcgggcca ggatccgccg ccgaatctcc
     1801 tctgctcctg gccgggccgt atcccacacc ccgaagctcc agcccatcac gcggtagccg
     1861 agcgccttcg cagcccgaaa gacgaaggga ttgcgatagc cgtgcggagc ccggaacatg
     1921 gtcggaatcg ttcccgaggc ctcgacgatc gcttggtggg cggagcgaag ctcccgctcg
     1981 attcgatggg gtcccagtcg atgcagcttg gcgtgtgtct gggtgtgatt gccgattcca
     2041 tggccggcgg cagcgatgcg ccgcgtgagc tctggatacc gcgacacatg ccgaccgacg
     2101 agaaaaaacg ttgccggcac accggcctcc gcgagcgtat cgagaattgc cggcgtcgcc
     2161 ccctcgttgg gaccgtcgtc gaaggtgaga tacaccgagc ggtctctcgg acctcggctg
     2221 gtcaccggac cgaacaggcg gttagtgggc tcgagagcac cgtaggtcag caaccccgcg
     2281 gcgccagcca atcccgccgc gacggcaacg cctatcattt cgtgacccgc tgatacacgg
     2341 caaacacttg ctcggcaact ctgggccatg cgaacgtttg agctttcgcc agtccggccg
     2401 agcgcatagc ggatcgacgg cgcggatcgg ctagaagatc gatcgtcacc tgagcccagg
     2461 cggtcgcatc gtctttgttc accagcacgg cctcgctatc gcgggcaacc agttcgcgaa
     2521 acccagtgat gtcggaaacg acgagcggcg taccgcaagc catggcctcg agcagcgtaa
     2581 tgccgaacga cgccttcgtg gtgggacaga gatagaggtc ggcggacccg tacacttctg
     2641 gccggttgcc gttcacccga ccgaggaagt cgacgtgttc ggcgaccggt cgggccaggc
     2701 gctcgtagag cgggcgcagc gggccatcgc ccgcgatcgt taacctggtc cgggggaacc
     2761 gttccaacac agccggcatc gcctgcagca cggtctccaa gccattgcgc ggatcaagcc
     2821 ggccaagaaa gagcagcttc gggtcctgaa cctcaggggc cgccggcgat cgtccattgg
     2881 gttggaagaa cgcggtgtcc acgccgttgg gaatgatctc ccagtcggcg tccacgtagc
     2941 gcgcatgagc ctccaccaca ggttggctta ccgcgatgtt ggccgcttgg tggcccagga
     3001 gccatttcgc ctcgctgcgc cagaaacgga gtgcgagaga tttggcgaac caggagtgaa
     3061 atgtcgccac gacgggaatg ccgaggtcgg aggcggccag tggagcgatg atcccgaagg
     3121 tcggagccag ccctccatgc acgtgcacga tgtcgacctg ctccctgcgg aacagatcgc
     3181 gcagctgccg tcgcagccgc catccggtcg tgatgcgggc gaaggacccg ctactgtaga
     3241 tgattcgact ggtgcccacc cgccggacga aatcctcgtc cttctcatac tcgtgagcgt
     3301 ccggagtccc cgtgggcatc cgcatgttcg aggtgacgac gatagccgga tgccctgcgg
     3361 cgttgtaaac cttggccaga ttgtggacgt gctcggttac gccgcctaag tgggggtagt
     3421 agaactcagt gacgagcgcg atgcgcatca agcccttcgc cttccattga gaaactgccg
     3481 taagggacgt ggccgccttg ggcaagaagg cggccactga gccaagttga ccgggctacg
     3541 gcagtatgga cttagagcct gccgagcgga ccaaatctaa acaggccggg actttctggc
     3601 ccacgaggac ggccgttttg cgccaagttg tccaagacgt tgagctccgg ccgctgtttc
     3661 gcgctttact caaactgggg tgtctgggtt tcgaaggcac tgccgcccac ctggctctga
     3721 tggaaaagga gatcgtttta gaccgccggt ggctcgagcc gggcgagttc ttggacctca
     3781 tggcgcggcg aacttgatac ccggtccgaa ctccagttgg tctgcgcaaa gggggctggc
     3841 gcgggatgct gctggccggc ctcggcttca tctctcccgc cgtgcttctg gtgttgatag
     3901 cggcgtgggc ctaccagcga tacgggcggc ttccccccgt cgaaggtatc ctccgtggtg
     3961 ggacccccgt tgtagtagcg gtcgtcaccc acgccaagtc ggagcggtgc gctcgtgtac
     4021 ggcagcggct acgtcctatt ggccttcctg ccgggtcccg tctttaccac cgcgacgtcg
     4081 ccggtatcgc cttgctggtg tgcccgcggg cctcgcggct accgccggta tcttcctccc
     4141 ggcgtttatt ttggtcccac ttagcggagc attgttacct cggctccatg cggctccagc
     4201 agcgcgtcgt tttctccacg gggtcaacgt cggggccgtc gcgctgctga tagggatcgc
     4261 gatcgaactt ggccgagccg gtctacatgg gccgggtact atcgcgctca cagtgctcgc
     4321 gctcttgttg ctgatgcgcg ggctgagtgc cccctggctt atgctggcgg tgctctactc
     4381 ggtctcgcgt ccgcgctcgg gtgacgacac cacatcgtcc gataagttcc aggatccgca
     4441 actttcgtta gtcgttccgc gcgactcagg agtgacggtg ctcgttccca agctcgccac
     4501 tacgctgaag gaatacacgc gccagcagtt tgcaaacgat ctggtggccg gggttattgt
     4561 cggggtagtt gcgctgcctc tggccattgc ctttgccatt gcaa
//
LOCUS       DASRJQ010000073         2358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1083473, whole genome shotgun sequence.
ACCESSION   DASRJQ010000073 DASRJQ010000000
VERSION     DASRJQ010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083473"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="VFB89_01720"
     CDS             complement(<1..985)
                     /locus_tag="VFB89_01720"
                     /inference="COORDINATES: protein motif:HMM:NF026066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26050.1"
                     /translation="MPRLSNAEIAKVLLGLAQLLSAQRENPFKIKAYRRAANAIANLS
                     QSVDELVRTDADLTAIPGIGRSIAGAIREIVQRGGALQQLEELRGKVGQQTAALNDYP
                     LLDPKRVARIYQKLRISSVAELKERLERGDIREQMGLRMEQHVRQGLSPTTEILLYEA
                     HQIVPVVDEFLRNECGAQRVEAAGDYRRRVEAVRELHFVVQSDNFQNVLDRFKSYGGR
                     ATPVSSETMTAAFQLPSGVQVRLSHTTEALWGLSLLIATGSERHLLKLLEMGHDLKTL
                     MGGNASGRTEEAIYRKLGLQPIPPELREGNDEVELSAAGRLPKLITATDLCG"
     gene            1009..1893
                     /locus_tag="VFB89_01725"
     CDS             1009..1893
                     /locus_tag="VFB89_01725"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZM26051.1"
                     /translation="MSDDKPSRTGQNLMMARLKRLAEGHPALGFTLFPMRLKTALSSY
                     VPTAGALARWLFRSREYTNFTYDYSPPSLEYLAHGLALVTGCPVGSLRAFLAEPDENA
                     ELHRTVRERGETSDRRAVIDRDVRLGKQRIWYALARALKPVRVVEAGVGPGLAAVVLA
                     EALLKNASEGNGGEYLGIDRDPRAGVLLGPRQRSVARLLHGESVSTLGSLGQAVDLFI
                     TDSHVSPDVEYAECRAVEPWLSEPAVVGTTVTTRLPAFAEETGRSFLAFKEEPRDHWY
                     PGGWIGLAFRASPGSVVP"
     gene            1893..>2358
                     /locus_tag="VFB89_01730"
     CDS             1893..>2358
                     /locus_tag="VFB89_01730"
                     /inference="COORDINATES: protein motif:HMM:NF021601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HZM26052.1"
                     /translation="MRLPRVVGGVMGRLPIGAWLARLEAARQSDASRRTAAVLLRVDD
                     YPHWRVPTPQFRRFQQVMSAIGVPFLLAATPFLAANPLSPISDTRPEDVNDWDWLAER
                     VRRKELEVGLHGATHRTRQFGFNSEFDDMPPDQARAVIGQAWRFLSSAGCEPI"
BASE COUNT          387 a          766 c          744 g          461 t
ORIGIN      
        1 cgccacaaag gtccgtcgcc gtgatcagct tcgggaggcg cccggccgct gacagctcca
       61 cctcgtcgtt gccctctcga agttccggag ggatgggctg tagaccgagt ttccggtaaa
      121 tcgcctcctc ggtgcgaccg ctcgcgttgc ctcccatcag cgtcttcaga tcgtgaccca
      181 tctcgagcag tttcagcagg tgccgctctg agccggtcgc aatgagcagc gagaggcccc
      241 acagtgcctc cgtcgtgtgg ctcaagcgta cctgtactcc cgacggcagt tgaaacgcag
      301 ctgtcatcgt ctcggagctg accggcgtgg cgcggccgcc gtaactcttg aatctgtcca
      361 gaacgttctg gaagttgtcg ctctgaacga cgaagtgaag ctcccggacc gcctccactc
      421 gccggcgata gtcccctgct gcctcgacgc gttgcgcccc gcattcgttc cgcaggaact
      481 cgtcaacgac cggtacgatc tgatgcgcct cgtacaggag aatctcagtg gtgggactta
      541 ggccctgtcg aacgtgctgc tccatccgca ggcccatctg ctcgcggata tcaccccgct
      601 ccagccgttc cttgagctcc gcgaccgacg aaatccgtag tttctggtag atccgggcca
      661 cccgtttggg gtcgagcagc gggtagtcgt tcagcgccgc cgtttgctga ccgacctttc
      721 cccggagttc ctcgagctgt tggagcgcgc cgccgcgctg gacgatctca cgtatggcgc
      781 cggcgatcga cctgccgatt cccggaatcg ccgtcagatc cgcatcggtt ctgaccagct
      841 cgtcgacgct ctgggagaga ttggcaatgg cattggccgc gcgacggtag gccttgatct
      901 tgaagggatt ctccctttgt gccgacagca gctgagccag gccgagcagg actttggcga
      961 tttcggcgtt actcaacctc ggcatgaatc tcctccctgg gagcctgaat gagtgacgac
     1021 aaaccgtcgc gaacgggtca gaatctgatg atggcgcggc ttaaacgtct cgctgagggg
     1081 catccggccc tcgggttcac gctcttccca atgcgattga agaccgcact ctcatcgtac
     1141 gtacccaccg cgggggcgct cgcgcggtgg ctgtttcgct cgcgggagta caccaacttc
     1201 acctacgact actcgccgcc cagcctggag tatctcgccc acggcctggc tctggtgaca
     1261 ggttgtcccg tcgggtcatt acgggccttt ttggccgagc ccgatgagaa tgccgagctg
     1321 catcggaccg tgcgcgagcg gggtgaaacg agtgatcgga gggcagtcat cgaccgtgac
     1381 gtacgcctag gaaaacaaag gatttggtat gcgctggcgc gggccctgaa gccggtgcgc
     1441 gtggtagagg ctggcgtagg tccgggactt gctgccgtcg tcctggcgga ggcgctgctc
     1501 aagaacgcga gtgaaggcaa cggtggtgaa tacctcggga tcgaccgaga ccctcgcgcg
     1561 ggagtcttgc tcggtccaag gcagcgctcg gttgccaggc tcctccacgg agaatcggtg
     1621 agcacgctcg gaagtctggg acaggccgtc gatctgttca tcaccgacag ccatgtgtcg
     1681 ccggacgtgg aatacgcgga gtgccgggcg gtcgagccgt ggctttccga accggctgtt
     1741 gtcggcacga cagtgacgac gcggctcccc gcctttgccg aggagaccgg acgctccttc
     1801 ctcgcgttca aggaggagcc tcgcgatcac tggtatcccg gcgggtggat cggccttgcc
     1861 tttcgggcct cacccggcag cgtggttccg taatgcgatt gccacgtgtg gtcgggggcg
     1921 tcatgggccg gctgccgatc ggagcctggc tcgcccgcct cgaggctgcc cgacagtccg
     1981 atgcatcccg ccgcacggcc gcagtcctgc ttcgggtaga cgactaccct cactggcggg
     2041 tccccacccc tcaatttcgt cgcttccagc aagtgatgtc ggcgatcggg gtgcccttcc
     2101 tgctagcggc gacgcccttt ctcgctgcca accccctctc ccctatttcc gacacccgcc
     2161 cagaggatgt caacgactgg gactggctcg ctgaaagagt acgtcggaaa gagctcgagg
     2221 tgggcttgca cggagcgact catcgaaccc ggcagttcgg gttcaactcc gagttcgacg
     2281 acatgccgcc ggaccaggcg cgagccgtca tcggacaggc gtggcgcttc ctctccagcg
     2341 cgggctgcga gccgatag
//
LOCUS       DASRJQ010000074         2104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1399699, whole genome shotgun sequence.
ACCESSION   DASRJQ010000074 DASRJQ010000000
VERSION     DASRJQ010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399699"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1250)
                     /locus_tag="VFB89_01735"
     CDS             complement(<1..1250)
                     /locus_tag="VFB89_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26053.1"
                     /translation="MTAGAPSPAMLLPSQVAELLQTMVKALRAFQMYLPNNPVHQRAA
                     QNVKAAFGPIWSAVDDLTLAVGETELRWEEEVVYSQLNKADCLAWMLFKDGMRVLTIR
                     KGADQDEVIRFLELLNHARFLPAEAGDDLMTLLWEQDFQLIQYHFIDLLGEAEPLEGS
                     PASVGGTLEPVAAETRRGQVAEEAPPRPKGIVDLEEFDSTLYFLEEAEINYVVEQVQE
                     EYRRDVRQASLNALFDLLELHTDDRIRIEILGVLDMLFPNLLNKGEFRTVAAVMRETR
                     LITSRAQNLTEEQRRRLQDFEAQLSQPGIVVQLVQSLDEAAALASDEDVTEVLRELRP
                     TALGALIDSLPRLNNASVRSLLEATVDRLATNHPAEVMRLLRNPPGQSLPALVALSGR
                     LGVQQAVPGLGELLANPDPAVRLAT"
     gene            complement(1247..>2104)
                     /gene="trpB"
                     /locus_tag="VFB89_01740"
     CDS             complement(1247..>2104)
                     /gene="trpB"
                     /locus_tag="VFB89_01740"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HZM26054.1"
                     /translation="TGAGQHGVATATVCARFGLECVVYMGTEDVERQALNVYRMRLLG
                     AKVIPVESGTKTLKDATNEALRDWVTSVPTTHYIIGSVVGPDPYPRMVRDFQSVIGRE
                     ARTQVLERHRRLPRTIVACVGGGSNAMGMFAGFLDDAAVELVGVEGAGEGIATGHHSA
                     TLGAGRPGVLHGSLSYLLQDADGQVAPAHSVSAGLDYPGVGPEHSWLRDTGRVRYESV
                     TDDEALAAFQRTSRLEGIIPALETAHALAWVFRSKGRWSGDQIVLVCLSGRGDKDVAH
                     AAALLGPDA"
BASE COUNT          376 a          704 c          643 g          381 t
ORIGIN      
        1 gttgcgaggc gtaccgccgg atcgggattc gccagcagct cgcccagacc cggaacggcc
       61 tgctgtacac ccaggcgacc cgagagcgcc accaacgcag ggagcgattg ccccggaggg
      121 tttcgcagca gccgcatcac ctcggcgggg tggttggtgg caagccggtc gaccgtggcc
      181 tcgagcaggg accgcaccga tgcgttattg agccgaggaa gtgaatcgat gagtgcgccc
      241 aacgctgtag gccgcaattc ccggagcacc tcagtcacat cctcgtcact cgccagcgcg
      301 gcggcttcat ccagtgactg gaccagctgg accacgatgc ccggctggct cagctgcgcc
      361 tcgaaatcct ggagtcgtcg tcgctgctcc tcggtgagat tctgcgcgcg actcgtgatg
      421 agccgggttt ctcgcatgac tgccgcgacc gtcctgaatt cacccttgtt gagcaggttg
      481 gggaacagca tgtccaggac gccgaggatc tcgatccgga tacggtcatc cgtgtgaagc
      541 tccagcagat cgaagagcgc gttcagcgag gcctgtctca catcacggcg atactcctcc
      601 tgcacttgct ccacgacgta attgatctcg gcctcttcca ggaagtacag agtcgaatcg
      661 aattcctcga gatcgacgat tcccttgggt cggggcgggg cctcctccgc cacctggcct
      721 cggcgcgtct ctgcggcgac cggctccaag gtgcctccga ctgaggcggg ggagccttcc
      781 agcggctcgg cctcacccaa cagatcgatg aagtggtact gaatgagctg gaaatcctgc
      841 tcccatagca gcgtcatcag gtcatcgccc gcttcagcgg gaaggaaccg cgcatgattg
      901 agcagctcga gaaacctgat gacttcatcc tgatccgcgc ccttgcggat cgtcagtacc
      961 cgcatgccgt ctttaaagag catccaggcc agacaatccg ccttgttcag ctggctgtac
     1021 acaacctctt cttcccagcg cagctcggtt tctccaaccg ccagggtcaa atcgtcgacc
     1081 gcactccaga tcggcccgaa tgccgctttg acattctggg cggcgcgttg atgaacgggg
     1141 ttgttgggga gatacatctg gaaagcgcgc agggccttga ccatggtctg gagcagctcc
     1201 gcgacctggc tcggcaggag catcgcaggc gagggtgcgc cagccgtcat gcgtccggtc
     1261 ccagcagggc ggccgcatga gcgacgtcct tatcgccccg cccgctcaga caaaccagca
     1321 cgatctggtc cccgctccac cggcccttcg atcgaaaaac ccaggccagc gcgtgagccg
     1381 tttcgagcgc gggaatgatg ccttcgagtc gagaggtgcg ttggaacgcc gcaagtgcct
     1441 catcgtccgt gacactctcg tagcgcaccc ggcccgtgtc ccggagccag ctgtgctccg
     1501 gaccaacccc tggatagtcg agtccggcgg agaccgaatg agcaggcgcg acctggccgt
     1561 cggcatcctg gagcaagtag ctcaagcttc catgtagtac accaggtcgg cccgcaccca
     1621 gcgtggcgct gtggtgcccg gtcgcgatgc cttctccggc cccctcgaca ccgaccagct
     1681 cgaccgccgc gtcgtccaaa aagcccgcga acatccccat cgcgttcgac ccgccgccga
     1741 cgcaagcgac aatcgttcga ggaagccgcc gatgtctttc cagcacctgc gtccgagctt
     1801 cccgtcctat caccgattgg aagtcgcgca ccattctggg gtagggatcg ggccccacta
     1861 cggatccgat gatgtaatgg gtggtcggca cgctggtgac ccagtcgcgc agtgcttcgt
     1921 tggtggcatc cttgagcgtc ttggttcccg actcgaccgg gatcaccttg gcgccgagga
     1981 ggcgcatgcg gtagacgttg agcgcctggc gttccacgtc ttccgtcccc atgtagacga
     2041 cgcactcgag cccgaagcgg gcgcacacgg tcgcggtcgc gacaccgtgc tggccggcgc
     2101 cggt
//
LOCUS       DASRJQ010000075         3324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3333330, whole genome shotgun sequence.
ACCESSION   DASRJQ010000075 DASRJQ010000000
VERSION     DASRJQ010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3333330"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..2505
                     /locus_tag="VFB89_01745"
     CDS             163..2505
                     /locus_tag="VFB89_01745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HZM26055.1"
                     /translation="MFSRITAPLVRAWHTPRVRRGVLVGVLALITLGFAVVLAAWTGA
                     CAGNTCPSIEGLDRYDPNQAAKVYAADGRLITDLGLERRTVVPLGEMSPYVKAAFIAT
                     EDKRFYEHKGIDWVRVFGAVKANVFKLRFAEGFSTITMQLARNLWPEDISGRDKSLRR
                     KLREVKVALEIERKYPKDKILELYLNQIDLGNRAYGVETASQRYFGKSVRDLNLAEAA
                     TLAAIPKAPSRYNPRRNPNLNLQRRNTIINLLRDDQALSERDAERWKAFPLLLSSHSD
                     FSGVAEYFVEYVRQQLDARFGPDLYREGLRIYTTLDLDMQQAAERALEARLEAIEDGA
                     DGKFTWQTYRQYLDAHADTPEDHGNTPTPYLQGLVVTLEAKTGAIRAMVGGRDFEDSK
                     FNRATQSRRQPGSTFKPIVYSAAIEAGYPLSYVIADDPLTVELPPPQGMWMPQNYDLE
                     FEGPMTLRRALMLSRNIVAVKLGMEVGEQAVIAEAAKFGLTTKVPPVPSIHIGSADVI
                     PLEMIGAYTTFANLGVRTVPTAILRVEDRSGKMLWQPQVKSEVVMDTMHAWLMNDALR
                     DVVRHGTAFGAVTARNFPAPAGGKTGTTNDGFDVWFIGFTPDLVTGVWIGFDQPRKIK
                     SNAQGGILAAPAWTAMMKEIYDRRPIPAAWPRPDGLAALDIDRSTGFKATPFCPKELH
                     YIESFIPGTEPTQFCPVHSPFNLGGNGASPMGGSPPPPATPNPPQPPSSGTLGGTTPR
                     PGQPGSPDPRATPAPGGVMGGSGPPPSSGGPPPSPRKPPN"
     gene            2507..>3324
                     /locus_tag="VFB89_01750"
     CDS             2507..>3324
                     /locus_tag="VFB89_01750"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZM26056.1"
                     /translation="MPAVRLSAGWVVPIEGPPISDGAVLVGADGRIAALGEDAQVPRP
                     DNGPVHHYPGAAIIPGLINTHTHLELTDLAGQVEEDDFPAWIRRLRELKEQSGTEQYL
                     TAAKRGVEACWASGVTTVADTGDRAVVLQALRECGGSGIAYQEVFGPHPDQAPTSMAQ
                     LRETVARLRSFESDRVRLGISPHAPYTVSGPLYRMAAEFAAMEGLPTAVHLAESLAET
                     QLLRAAAGPFADAWQRRGIPLPVMPGRSPVAWLDEHGALTERTLCIHVVQLDAG"
BASE COUNT          606 a         1089 c         1053 g          576 t
ORIGIN      
        1 gcggtcggcg tagagctgga gaaggtggga catgggaaaa gcgtaaacgg tgaacggtga
       61 acggtaaaca ggggtggctg ggagggcccg ttcaccgttc accgttcacc gttcacgccc
      121 ttgtagctgt tctagcccgg gtcgcccggc taatttcggg tcatgttctc ccgcataacc
      181 gcacccctgg tccgggcctg gcacacgccg cgggtccgac gaggggtgct tgtcggcgtc
      241 ctcgcactga taacgctcgg attcgcggtg gtgctggcgg cgtggaccgg cgcctgtgcc
      301 ggcaacacct gtccctcgat cgaagggctc gatcgctacg atcccaatca ggcggcgaag
      361 gtctatgctg ctgacgggcg cctcatcacc gatctcgggc tcgagcgtcg cacggtcgtg
      421 ccactgggag aaatgtcgcc gtacgtcaag gcggcgttca tcgcgaccga ggacaagcgg
      481 ttctacgagc acaagggcat cgactgggtc cgcgtcttcg gcgcggtgaa agcgaatgtc
      541 ttcaagctgc gtttcgccga gggtttttcc accatcacca tgcagctggc ccgcaatctg
      601 tggcccgagg acatcagcgg acgggacaag tcactgcggc gaaagctcag ggaagtaaag
      661 gtcgccctcg agatcgagag gaaatacccc aaggacaaga ttctggagct ctatctcaat
      721 cagatcgacc tggggaatcg cgcgtacggc gtcgagactg cgtcgcagcg gtattttggg
      781 aagtccgtgc gggatctcaa tctcgcggaa gcagccacgc tcgcggccat tccgaaggcg
      841 ccctcgcgct acaacccgcg ccgcaatccg aacctgaatc tccagcgacg caacacgatc
      901 atcaatctgc tccgcgacga ccaggcactc tccgagcgag atgccgagcg atggaaggct
      961 tttccgctgc tgctctcgtc ccactcggac ttcagcgggg tggccgagta cttcgtggag
     1021 tacgtccgcc agcaactgga cgcccgcttc ggtccggacc tctaccgcga gggcctgcgg
     1081 atatacacta cgctcgatct cgacatgcag caggccgccg agcgcgccct tgaagcccgg
     1141 ctcgaggcaa ttgaagacgg ggccgacggc aaattcacct ggcagaccta tcgccagtac
     1201 ctcgacgccc acgcggacac gccggaggat cacggcaata cgcccacgcc gtaccttcag
     1261 gggctcgtgg tcacgctcga ggcgaagacc ggcgcgattc gcgcaatggt gggaggacgc
     1321 gacttcgagg acagcaagtt caaccgggcg acccaatccc gccgccagcc gggctccacc
     1381 ttcaagccga tcgtgtattc agccgcgatc gaggccggtt acccgctctc ctacgtaatc
     1441 gccgacgatc cgctcacggt cgagctcccg ccgccgcagg gcatgtggat gcctcagaac
     1501 tatgatctgg agttcgaggg gccgatgacc ctgcggcgtg cgctcatgtt gtcgcgcaac
     1561 atcgttgcag tcaaactggg tatggaggtg ggtgagcagg ccgttatcgc ggaagcagcg
     1621 aagttcggcc tgacgaccaa ggtcccgccg gttccctcaa ttcacatcgg ctccgccgat
     1681 gtcattccgc tggagatgat cggggcttac accaccttcg ccaatcttgg tgtccgcaca
     1741 gtgccgactg cgatcttgcg ggtcgaagat cgcagcggga agatgctctg gcagccgcag
     1801 gtgaagagcg aagtcgtgat ggatacgatg cacgcctggc tcatgaacga cgctctccgc
     1861 gacgtggtcc ggcacggcac tgcgttcggt gccgtcaccg cgcgcaactt cccggcaccg
     1921 gccggcggca agaccggaac gaccaacgat ggcttcgatg tctggttcat cgggtttacc
     1981 ccggacctcg tgaccggcgt atggatcggc ttcgatcagc ccaggaagat caagagcaac
     2041 gcgcaaggag gcatcctcgc tgcccctgcg tggaccgcga tgatgaagga gatctacgat
     2101 cgacgcccca ttcccgccgc ctggcctcgg cccgacggcc tggccgctct cgatatcgac
     2161 cggagcacgg gtttcaaggc tacgccgttc tgtcccaaag aacttcacta catcgaatcg
     2221 ttcattccgg gtaccgagcc cacccaattc tgtccggtcc actcgccctt caacctgggg
     2281 ggtaacgggg cgagtccaat gggcgggtcg cctccacccc cagctacgcc caacccacct
     2341 cagcctccga gttcgggcac gttaggcgga accactcctc gtccggggca gcccggctcg
     2401 ccagatcccc gtgccacccc tgcaccgggc ggtgtcatgg gcggaagtgg accccctccc
     2461 tcgagtggtg gacctccgcc gtcacccagg aagcctccta actgagatgc ccgccgtccg
     2521 gctctccgcc gggtgggtcg tgccgatcga agggccgccg atctccgacg gggctgttct
     2581 cgtcggagca gacggccgga tcgccgcgct cggtgaggat gctcaggtgc cgcgccccga
     2641 caacgggccc gtgcatcact accctggtgc cgcgatcatc cccgggctca tcaatactca
     2701 cacccacctc gagctgaccg atctggccgg acaagttgaa gaggacgact tcccggcatg
     2761 gattcgccgg ctgagggagc tcaaggagca gagcggcacc gagcagtacc tgacggcggc
     2821 caagcgcggg gtggaagcct gttgggcctc cggggtcacc accgtcgccg ataccggaga
     2881 ccgtgctgtc gtcttgcagg cgctcaggga atgcggcggt tccggtatcg cctaccaaga
     2941 agtctttggc ccgcatccag accaggcgcc gaccagcatg gcccagctcc gggagacggt
     3001 tgcaaggctg cgttcattcg agtcggaccg agtccgcctc ggcatctcac cccatgcgcc
     3061 atacaccgtg agtggtccct tgtaccgaat ggcggcagag ttcgcggcga tggaggggtt
     3121 gccaacggcc gtgcatctgg ccgaatcgtt ggcggagacg cagcttctcc gtgcggcagc
     3181 cggaccattt gcggacgcgt ggcaacgacg gggcattccg cttcccgtga tgccgggtcg
     3241 ctcaccggtc gcatggcttg acgagcatgg cgcgctgacc gagcgaacgc tgtgcattca
     3301 cgtggtgcag ctggatgcgg gcga
//
LOCUS       DASRJQ010000076         8174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_228221, whole genome shotgun sequence.
ACCESSION   DASRJQ010000076 DASRJQ010000000
VERSION     DASRJQ010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 8174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8174
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228221"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VFB89_01755"
     CDS             <1..263
                     /locus_tag="VFB89_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HZM26057.1"
                     /translation="SAMFASGTPSTFLSEVVQKTWLVVDEEGTEAAAVTGITMEATSA
                     LVRRPPIPFVVNRPFIYALRDTKTGLVLFLGLVRDPSAASHS"
     gene            359..1441
                     /locus_tag="VFB89_01760"
     CDS             359..1441
                     /locus_tag="VFB89_01760"
                     /inference="COORDINATES: protein motif:HMM:TIGR03805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="parallel beta-helix domain-containing protein"
                     /protein_id="HZM26058.1"
                     /translation="MRRIIAPSLLLLAACGQDQPTEPVTQPAVRATEVAAGSSIQAAI
                     DAAGAAEVIHIRPGLYREALRIDKPGIKIIGQSTAAGGVVLENPANADNGIVVTTNGD
                     GFALSDITVRGFEENGVLLVGVDGFSLTRVTTERNGEYGLFPVRSSHGVIKDCSASGH
                     TDTGIYVGQSQGIEVSNSLAFDNVIGIEIENSSDVRVSGNRMRDNTAGMLAVLLPGLN
                     VKTSSNVVLTGNDVRDNNRPNAGAPGELESFVPAGSGILVVGPDAVTADHNTVTGNGF
                     VGIGVASTLVLGALAGLPPEAFGDIEPNPDGGRVMSNTVRGNGGSPPPIPLPGAELFW
                     DGSGTGTCWDKNDFGSSVPSPLPACR"
     gene            1506..2213
                     /locus_tag="VFB89_01765"
     CDS             1506..2213
                     /locus_tag="VFB89_01765"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HZM26059.1"
                     /translation="MRLSVGTSGYAYKEWKGAFYPPKLPADEMLRFYAGRLPAVEINN
                     TFYRMPTERVLLDWTTQVPEGFRFVLKAPQRITHYARLKLEDDSLEYFLRTANVMGGK
                     LGPTLFQLPPNFKKDLSRLQEFIAKLPRTWRAAFEFRHEGWFTDEVFDTLRGRDLALV
                     TVDDDEGESPLVPTASWGYLRLRRTNYDRPALAGWSERIRAQPWQEGYVFLKHDEEGT
                     AANGPRWAEELLQLAST"
     gene            complement(2244..3104)
                     /locus_tag="VFB89_01770"
     CDS             complement(2244..3104)
                     /locus_tag="VFB89_01770"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZM26060.1"
                     /translation="MRISLLGVAFTCLAFPLAPLAAQHSGPSDRGRLGIFLDEGADSG
                     GARVRGVVPDGPAEKAGIERGDVILRFNGTALNESQKLVDLARRLQPGDTVKLQYRRG
                     SETRTASLVADRMRPRFAMMFDRMGGPHFGMGVGPDMMYFRSHGRSGLALVQLSKDLG
                     EYFGTSEGMLVIRPPADSASPLKAGDVILSIDGRKPQSVEHALRILGSYAPGEKAKLD
                     IMRKKQRTSLTWTAPALRTEHDGWKRGGRHSMEPSEKLDSWGAMDFEIPVPSPDDFML
                     SISPSETQET"
     gene            complement(3170..4684)
                     /locus_tag="VFB89_01775"
     CDS             complement(3170..4684)
                     /locus_tag="VFB89_01775"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HZM26061.1"
                     /translation="MQPQAAYDELIRLAREEALLSSCVDLLEWDEETCLPRGGVAHRS
                     EQLALLAGLLHDRSTDPRLGELLDVVEGSSLVSEVESAAAVNAREIRRGYDRERKLPR
                     KLVAELARVTALAQQAWAGAREASDYNAFRPWLERILKLAREEAEAVGYPETAYDALL
                     DDYEPGMTAAETASLFEELKRELLPLVETITAGRKPRARSLSQRREFAVDRQRLFGEA
                     VAAAVGFDLERGRLDTSLHPFCTGLGPGDCRITARFSSRDFAGGFFAVLHETGHALYE
                     QGLESEHYGTPMGEAASLGIHESQSRLWENLVGRSRSFWQHFYPRARAVFHDAMHDLK
                     LDTFLAGINRVEPSCVRVRADEVTYNLHILIRFELEQALIAGDLATADVPEAWDAAYK
                     RYLGVVPSSPVEGCLQDGHWAEGLIGYFPTYTLGNLYAAQLFAAARADLGDLDAQFAR
                     GDFVTLLGWLRDRVHRHGQRYRPKQLVEHATGAPPDWRPLIAALKAKYLDVEGK"
     gene            4724..6211
                     /locus_tag="VFB89_01780"
     CDS             4724..6211
                     /locus_tag="VFB89_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26062.1"
                     /translation="MEAEKPVSQEEQPQVDLSPAEAAGLAGMRLAAHVRQALYRIPTI
                     RLAEAHRHMQRKAAERRVAYFREGEMDTIRILPCPIAVLPDEVEYLRFVCRTLQGALA
                     RLPDLYLSDPGVHEVLRLYPEEEEWLRACWGAPQAQRNPMFSRLDAMIDYPNPMWKET
                     LWFVEPNLTGIGGVYLIPTIEEIVSEEIVPLLREQDAGLDLRRLSDARELLFSEISQQ
                     LSATGRRGRCVCFVEPKYEGEGIDEQRSLVEYFRARHGWDTVHADPAELRLENGEVRY
                     EDHVVDLVYRDYSVLDLVDLGSQGVDVEPMRRLFRENRVVSSIGAELDQKSCWEVFTD
                     AALAERHFTAEERGVFRRHILWTRLLGDRWTQLPGGEEGDLLDFTRRERETLVLKPNR
                     GYGGDGVTIGHTLSQSDWERAIDTALSDEERWVVQRLAAIPVVEFPTLDESGSLQPEP
                     FYTVMGFAPGANGVAILARASQRQVVNVAQRGGMCAVLEAAATVV"
     gene            6249..7361
                     /locus_tag="VFB89_01785"
     CDS             6249..7361
                     /locus_tag="VFB89_01785"
                     /inference="COORDINATES: protein motif:HMM:NF012246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016984 - ribulose-bisphosphate
                     carboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HZM26063.1"
                     /translation="MSEPIRVSYLVDCDADRIRARAEALLLEQTVELPRKAVRDPAVA
                     QYIIGQVEHIEPIGERRFRVIIAQPLAAAAGDPAQLLNLLFGNSSLQPDLVLEQVALP
                     DETLSFLSGPRYGLAGLRTLSGVTDRPLLATALKPMGLTPEALGQLARQFALGGLDVV
                     KDDHGLADHAVCPFERRVEACLSAVSAVYQDTGHRTLYVPNLIGTPERVRDQLRFARS
                     AGAPAVMLSPMLLGLPSLAELVGHAELPVIAHPAFAGVLRATEPALLGTLFRWYGADG
                     VIFPHVGGRFSYSLETCGALARTLRAPHRGMKPALPIPAGGIHLDRVADLIAFYGTDC
                     MLLIGGSLYEAGDRLIETTRALVERVATVAQQEETK"
     gene            7358..7816
                     /locus_tag="VFB89_01790"
     CDS             7358..7816
                     /locus_tag="VFB89_01790"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZM26064.1"
                     /translation="MSGPEERTHVSESAPFRWEGIPVLAYKEEGSHFRDISRQVLFED
                     ESLGAELRYFEIAPGGWSSLERHHHMHAVMIVRGEGRALVGDRIVDLKPHDLVRVPPL
                     TWHQFRAGPNDSLGFLCLVACNRDRPERPDEEAVKGLRANREVGEFVRMG"
     gene            complement(7917..>8174)
                     /locus_tag="VFB89_01795"
     CDS             complement(7917..>8174)
                     /locus_tag="VFB89_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZM26065.1"
                     /translation="SDEGTDAATLAKSRTSDLETALASLPPRDAKVIRLYFGLDDGRA
                     RTLEEIGRMMGVTRERIRQLRDRALKQLREQHGDRLKDLAA"
BASE COUNT         1449 a         2600 c         2661 g         1464 t
ORIGIN      
        1 ttagtgcgat gttcgcgagc ggcacaccca gcacgttcct gtcggaggtg gtccagaaga
       61 cctggctggt ggtcgacgaa gaggggaccg aggccgccgc ggtaaccgga atcacgatgg
      121 aggcaacctc ggcgctagtc aggcggcccc cgattccctt cgtggtaaac cggccgttca
      181 tctacgctct gcgcgatacc aagacggggc tggtgctttt cctcggcctg gtcagagacc
      241 catcggctgc cagccacagc tgacggctga tcgctctcgg atagctgata gctgatagct
      301 ctttaccccc ccttgatccc tccagctcta catggtgaac caccttacgg aggttcccat
      361 gcgccgcatt attgccccgt ctctcctgct gctcgccgcc tgtgggcagg accagcccac
      421 cgaacccgtt acccaaccag cggtccgcgc gacagaggtc gcagccgggt cctcgattca
      481 ggccgccatc gatgcggccg gcgctgccga ggtgatccac atccgcccgg gtctctatcg
      541 cgaagcgctc cgcatcgaca agccgggcat caagatcatc ggccagtcga ccgcagccgg
      601 cggtgtcgtg ctggaaaacc ccgccaatgc cgacaacggc atcgtcgtga cgacgaacgg
      661 agacggattt gccctgtctg acataaccgt gcgtgggttc gaggagaacg gcgtgctgtt
      721 ggtcggggtt gacggctttt ctcttacccg ggtcaccacc gagagaaacg gggagtacgg
      781 ccttttcccg gtgagatcca gccacggcgt aatcaaggac tgctctgcca gcggccatac
      841 cgacaccggg atttacgtgg ggcagtcgca aggcatcgag gtgagcaaca gcctggcatt
      901 cgacaacgtt atcggcatcg agatcgagaa ctcgtccgac gtccgcgtct caggtaatcg
      961 aatgcgggac aacactgcgg gcatgctcgc agttcttctg cccggtctca acgtcaagac
     1021 gagcagcaac gtcgtgctga ctgggaacga cgttcgcgac aacaatcgcc ccaacgccgg
     1081 ggcgcccggt gagctcgagt cgttcgtgcc ggcgggctcg ggtatactgg tggtcggtcc
     1141 ggacgcagta acggcggatc acaacactgt cacgggaaac ggcttcgtcg gcatcggtgt
     1201 ggctagcacc ctggttctgg gtgcgctggc cgggttgccg ccggaagcat tcggggatat
     1261 cgagccgaat ccggatggcg ggcgagtcat gtcgaatacc gtgcgcggga acggtggatc
     1321 gccgccaccc atcccgcttc caggagccga gttgttctgg gacggctcgg gcaccgggac
     1381 ctgctgggac aagaacgact tcgggtccag cgtgccgagt ccgctgcccg cgtgtagata
     1441 gcgcgaagcc gatctacccg gcgccctcgc atccggcgat gttggggtgc aggacggagg
     1501 tttgaatgcg gctgtctgtc gggaccagcg ggtatgcgta caaagaatgg aagggtgcgt
     1561 tttacccgcc gaagctcccg gcggatgaaa tgttgcgctt ttacgccggg cgtctgccgg
     1621 cggtagagat aaacaacacg ttctaccgca tgccgaccga gcgggtgctg ttggactgga
     1681 ccactcaggt cccggagggg ttccgcttcg tgctcaaggc cccgcaacgg atcacccact
     1741 acgcccggct caagctcgag gatgattcac tggagtactt cctccgcacc gcgaacgtga
     1801 tgggcgggaa gctgggaccc acgctgtttc agctcccgcc caatttcaag aaggaccttt
     1861 cgcgacttca ggagttcatc gccaagctac cacggacctg gcgggccgca ttcgaattcc
     1921 gccacgaagg ctggttcacc gacgaggtgt tcgacaccct ccgaggccgc gacctcgccc
     1981 tggtgacggt tgatgacgac gagggcgagt ctcccttggt cccgacggca tcctggggct
     2041 acctccgtct ccggcgaacg aactatgacc ggcctgcact cgccggatgg tcggagcgga
     2101 tcagggcgca gccttggcag gaggggtatg tctttctgaa gcatgacgaa gaggggaccg
     2161 cggcgaacgg tccccggtgg gcggaagagc tgctgcagct tgccagcacg tgacgaccgc
     2221 ttccgccgag cggcctcgaa ttctcaggtc tcctgcgtct ccgatggcga tatcgatagc
     2281 atgaagtcgt cgggcgaggg caccggaatc tcgaaatcca tcgcgcccca agagtccagc
     2341 ttctcgctcg gctccatgct atggcggccg ccccgtttcc acccatcgtg ctcagtgcgc
     2401 agcgcggggg cggtccaggt caacgaggtc cgctgcttct tgcgcatgat gtcgagctta
     2461 gccttctcgc cgggcgcata ggagccaagg atcctgagcg cgtgctcgac ggactgcggc
     2521 ttgcgtccgt cgatcgagag aatgacgtcg cccgccttga gaggcgaagc cgagtcggcc
     2581 gggggtcgga tcaccagcat cccttcactg gtgccgaagt actcacccag atccttactg
     2641 agctgaacca gggcgagccc gctgcgaccg tgggatcgga agtacatcat gtcggggccg
     2701 acacccattc cgaagtgcgg gccgcccatc cggtcgaaca tcatcgcgaa tcgtgggcgc
     2761 atccggtcag ctacgaggct cgcggtccgg gtttcgctgc cccggcggta ttggagcttg
     2821 acggtatcac ccggctgcaa acgacgggca aggtcgacga gtttttggct ctcgttcagg
     2881 gcggtcccgt tgaaccgcag gatcacatcg ccccgctcga tgccggcctt ttccgccggc
     2941 ccatccggca cgacgccccg cacccgggcg ccgccactat cggccccttc atccaggaag
     3001 attcccagcc gaccgcggtc cgaggggccg ctgtgctggg ccgcaagtgg agccaacgga
     3061 aaggcgagac acgtaaacgc cacgccaagc agtgaaatgc gcattgggtt ctccggtccg
     3121 gggtgctggt ttcctatgga tgccgggacc gggagcagcg tcgcggcctt tacttcccct
     3181 cgacgtccag gtacttggcc ttgagcgcgg cgatgagggg tcgccagtca ggcggggcgc
     3241 cggtggcatg ctcgaccagc tgcttgggcc ggtaccgctg gccatggcgg tgcactcggt
     3301 cccggagcca gcccagcaac gtgacgaagt ctcctcgcgc aaactgtgcg tcgagatcgc
     3361 ccaggtcggc acgcgcggcg gcaaacagtt gggccgcata aaggttaccc agcgtgtagg
     3421 tagggaagta cccgatcaaa ccttcggccc aatgcccatc ctggagacaa ccttcgaccg
     3481 ggctggacgg caccaccccc agataacgct tgtatgccgc atcccaggct tctggaacgt
     3541 cagccgttgc cagatcgccc gcaatcaacg cttgctcgag ctcgaatcgg atgaggatgt
     3601 gcaggttgta ggtcacttcg tcggcacgca cgcgaacaca cgatggttcg acccgattga
     3661 tcccggctag aaacgtgtcg agcttgagat cgtgcatcgc gtcatggaat acggcgcgag
     3721 ccctcgggta aaagtgctgc cagaagctcc ggctccggcc gaccagattc tcccagagcc
     3781 gggactgcga ctcgtgaata cccagcgacg ccgcctcgcc cataggcgtt ccgtagtgct
     3841 ccgactcgag gccttgctcg tacagagcgt ggccggtctc gtgaagtact gcgaagaacc
     3901 cgccggcaaa gtcgcgactc gagaacctcg ccgtgatgcg gcagtctcca ggtccgaggc
     3961 cggtgcagaa tgggtggagc gaggtgtcca atctgccgcg ctcgagatcg aagcccaccg
     4021 ccgccgcgac ggcctcacca aacaggcgct ggcggtccac cgcgaattcg cgtcgctggc
     4081 tcaggctgcg ggctctcggc ttgcggccgg ccgtaatggt ctcgaccagg ggcagcaatt
     4141 ctcgcttgag ttcttcgaac aggctggcag tctcagccgc ggtcattccc ggctcgtagt
     4201 catcgagcaa ggcgtcgtag gcggtttcgg gatatcccac ggcttcggct tcttcacgcg
     4261 cgagcttcaa gattcgctcg agccagggcc ggaatgcgtt gtagtcgctc gcctcgcgcg
     4321 cgccggccca ggcctgctgc gccagcgccg tcacccgggc caactctgcg accagcttgc
     4381 gcggcagctt gcgctcccga tcgtagcccc gccggatctc ccgggcgttc accgccgcgg
     4441 ccgactcgac ttcgctcacc agactggaac cctcgaccac gtcgagcagc tcgcccaggc
     4501 gaggatcggt gctgcgatcg tgcagcaggc cggccagcag cgcgagctgc tcgctgcgat
     4561 gggcgacccc accacgaggc aggcaggtct cctcgtccca ttcgagcaga tcgacgcagg
     4621 aagagaggag agcttcctcg cgggcgagac ggatcagctc gtcgtaagcc gcttgtggct
     4681 gcatttgcgc attggacaaa tcggcgaaaa gatactcatc ccgatggagg ccgagaaacc
     4741 cgtgtcacag gaagagcaac cgcaagtcga tctctctccc gccgaggcgg cagggctcgc
     4801 cgggatgcgc ttggcggcgc acgtccggca ggccctctat cgcattccca cgattcgact
     4861 cgccgaagcg caccggcaca tgcagcggaa ggcggctgaa cgcagggtcg cgtatttccg
     4921 tgagggggag atggatacta tcaggattct cccctgccct atcgcggtgt tgccggatga
     4981 agtggagtat ctccgattcg tctgtcgcac cctccaggga gcgctggcgc ggctgcccga
     5041 tctctacctg tcagacccag gcgtccacga agtactgcgg ctgtatccgg aagaagagga
     5101 gtggctgcgg gcttgctggg gcgcgccgca ggcgcagcgt aacccgatgt tctcccggct
     5161 cgacgccatg atcgactacc ccaacccgat gtggaaggag accctctggt tcgtagagcc
     5221 gaacctgacc ggcatcggcg gcgtgtacct gatccccacg atcgaggaga tcgtaagtga
     5281 ggaaatcgtc cctctgctga gggagcagga cgccgggctc gaccttcggc gactctccga
     5341 cgcgcgcgag cttctcttca gcgagatctc gcagcagctc agcgctaccg gacggcgagg
     5401 ccgctgcgtg tgctttgtcg agcccaagta cgaaggcgag ggaatcgacg agcagcggtc
     5461 cctggtggag tactttcggg cccgccacgg ttgggacacc gttcacgccg atccagccga
     5521 gctccggctg gagaacggag aggttcgcta cgaggaccac gtggtggacc tggtgtatcg
     5581 ggactacagc gtcctcgacc tggtggatct cggatcccag ggtgtggacg tcgagccgat
     5641 gcgccggctg tttcgcgaga atcgggtggt ctcctcgatt ggcgccgagc tggaccagaa
     5701 aagctgctgg gaagtcttta ccgatgctgc cctggccgag cggcatttca ccgccgagga
     5761 acgaggtgtc tttcggcgcc acatactctg gactcgtcta ctcggcgacc gatggaccca
     5821 gcttcccggc ggcgaagaag gcgacctgct cgatttcacc aggcgagagc gcgagacgtt
     5881 ggtcctcaag ccgaaccgcg gctacggcgg tgacggcgtc accatcggac acaccctgtc
     5941 ccagtccgac tgggaacgcg ccatcgatac ggccttgagc gacgaagagc gctgggtggt
     6001 ccaacggctc gcggcgatac ccgtagtcga attccccact ctcgatgagt caggctctct
     6061 gcagcctgag ccgttctaca cggtgatggg gtttgcgccc ggggccaatg gagtagccat
     6121 actcgcgcgc gcatcgcagc ggcaggttgt gaatgtagct caacgaggcg ggatgtgtgc
     6181 cgtgctggaa gctgcggcaa ccgtagtctg agccgtggcg gcgtgaggag agtggccgga
     6241 gcgggcggat gagtgagccg atccgggtca gctatctggt cgattgcgac gccgatcgga
     6301 tcagggctcg tgccgaggcg ctcctcctgg aacagacggt cgagttaccc cgaaaggcgg
     6361 tgcgcgaccc ggccgtcgct cagtatatca tcggccaggt ggagcacatc gagccgatcg
     6421 gcgagcgtcg ctttcgagta atcattgcgc aaccgctcgc tgccgcggcc ggcgatccgg
     6481 cccaactcct caacctcctg ttcggtaatt cgtcgctgca gcccgacctc gtgctcgaac
     6541 aggtggcact acccgatgag acgctgagct tcctgtccgg gcctcgatac ggacttgcgg
     6601 gtctgcggac gctgagcggg gtcaccgatc ggccgctcct cgcgacggcg ctcaagccca
     6661 tggggctaac tcccgaggcg ctcggccaac tggcccgcca gtttgcgctg ggcggcctcg
     6721 acgtcgtaaa agatgatcac gggttggcgg accatgcagt ctgtccgttc gagcggcggg
     6781 tcgaggcgtg cctcagtgcg gtaagcgcgg tctaccagga caccggtcat cgaacgctct
     6841 atgtgcccaa cctgatcgga acgccggagc gggttcgcga tcagctgcgc ttcgcgcgat
     6901 ccgccggcgc cccggccgta atgctgtcac ccatgctgct cggcctcccg tcccttgcag
     6961 agctggtcgg gcacgccgag ctgcccgtca tcgctcatcc cgcatttgcc ggggttctca
     7021 gggccacgga acccgcgctc cttggcacgc tgtttcggtg gtatggtgcg gacggcgtca
     7081 tctttcccca tgtcggcggt cggttcagtt actccctgga gacctgcggc gcgctcgccc
     7141 ggacgcttcg agccccgcac cgcggaatga aaccggcttt gccgattccc gcaggtggca
     7201 ttcatctcga ccgcgtagcc gacctgatcg cgttctacgg aacggattgc atgttgctga
     7261 tcggcggcag tctgtatgaa gcgggcgacc gtctgatcga gaccacccgc gccctggtcg
     7321 agcgcgtggc gacagtagcc cagcaggagg agaccaaatg agtggaccgg aagagcgaac
     7381 tcatgtctcg gaatcggccc cgttccgctg ggaggggatt ccggtgttgg catataagga
     7441 ggagggcagt cacttccggg acatcagccg tcaggtcctg ttcgaggacg aaagtctggg
     7501 agcggagctg cgctacttcg agattgcacc cggtggatgg tcatcgctgg agcgtcatca
     7561 ccatatgcac gcggtgatga ttgtgagagg tgagggccgc gcgctggttg gcgaccgaat
     7621 cgtcgatcta aagccgcacg acctggtccg ggtgccgcca ctcacctggc atcaattccg
     7681 ggcgggaccg aacgacagcc tgggctttct ctgcctcgtg gcgtgcaatc gagaccgacc
     7741 cgagcgcccg gacgaagagg cggtgaaggg gttgcgggca aaccgggagg tcggggagtt
     7801 cgtgaggatg ggataaacgg agaaagggga acggggaaaa ggggaacggt gaagcaaacg
     7861 ggggctcact atgcgcggag cccccgttca ccgtccactg ttcacgcttt ttccaattac
     7921 gctgccaggt ccttcagccg atcgccgtgc tgctcgcgca gctgcttgag tgcacggtcc
     7981 cgcagctgcc ggatccgctc gcgcgtgacc cccatcatcc ggccgatctc ttccagcgtc
     8041 cgcgcgcgac catcatcgag cccgaagtag agccggatga ccttggcatc cctcggagga
     8101 agagatgcga gcgccgtctc gagatcgctg gtacggctct tcgccagcgt cgctgcatcg
     8161 gttccttcat ccga
//
LOCUS       DASRJQ010000077         2862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2756410, whole genome shotgun sequence.
ACCESSION   DASRJQ010000077 DASRJQ010000000
VERSION     DASRJQ010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2756410"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..845)
                     /locus_tag="VFB89_01800"
     CDS             complement(129..845)
                     /locus_tag="VFB89_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26066.1"
                     /translation="MKWWLAAVEAGVAAGTITAAGCSNLSTVESGIAGLEIRVPRPAA
                     VEVGQTIQLHARALDERGDSIAATVRWITPDTTVTLTPDGKLTGRTGGSTAQVQAQSA
                     NIVSDFVSFTVNARPDTIVITGDSILVVAAGAAASTPLLASLRSFAPAGPLSGRTILY
                     QIVEPVFPDPAQRTVELPGGVLAVNAQTGADGTPATAVTVNRISGRTAPDSAIVTVSA
                     TTATGAVVPGSSQRFIVRFQ"
     gene            complement(842..1285)
                     /locus_tag="VFB89_01805"
     CDS             complement(842..1285)
                     /locus_tag="VFB89_01805"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM26067.1"
                     /translation="MSFWRRVFGRESKEQLKPQRLDYLNEGLALERQGDFGAALTSYR
                     LAYRDNPNDSRILLNMAIALTKTGQPDEAIRYYRRALDIDPTLGGAHYGVAFLLLKRG
                     DTQQAADHLREFLARPPKGPDADQWVRHAQTALRDIAGSPSSETL"
     gene            1504..2013
                     /locus_tag="VFB89_01810"
     CDS             1504..2013
                     /locus_tag="VFB89_01810"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HZM26068.1"
                     /translation="MRRTTTPSPRGVLEVDWPFFGELCRALALRVAREYQPEVVLGVA
                     KAGVIPGVVVASILQCDFASMVVTRRESDADPILVAGPPASIRGRRVLVVDETCDTGN
                     TLKLALSEVRALKPREVRTAVSFKTGEYSPDFAAFTTEKLIILPWDREIIQGGELVMR
                     PDYAAWLGS"
     gene            complement(2010..>2862)
                     /locus_tag="VFB89_01815"
     CDS             complement(2010..>2862)
                     /locus_tag="VFB89_01815"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZM26069.1"
                     /translation="SGLPRGVPIRRDLLPIVRNALKDRYTVHREIGRGGAARVFLADD
                     RGGRSVALKVLHPELQVSVTAERFLREIRLASRIDHPLVARVLDSGEADWLVYYVMPF
                     IEGPTLRIQLDRGRQLPVDAAVRMTRDLLGALKAAHAVGIIHRDVKPENIVLSDAGAV
                     LLDFGIARAIEAAGTTQLTRSGIAVGTSSYMSPEQVQAATDIDHRTDLYSVGCVLFES
                     LAGRPPFQHPNEVVVLQMHLNDPVPDVRRFRPETPADVADVVMRSLAKSRDERWASAE
                     RMATALG"
BASE COUNT          488 a          922 c          936 g          516 t
ORIGIN      
        1 aggtcggccc cgctgatgtc ttcccagcgc tcgccctgct tgcgtcgcat gacgacgggc
       61 cggttcgccg cccggtccat tgcgccgaag aagagctggt tgagattgac gggctcggcc
      121 atggcggtct actggaatcg aacgatgaat cgttggctgg atccgggcac caccgcgccg
      181 gtcgccgtcg ttgcgcttac cgtgacgatg gcgctgtccg gtgccgttct gccggagatc
      241 cgattgaccg tgactgcagt cgcgggcgtg ccgtccgccc ccgtctgggc attcaccgcg
      301 aggaccccac cgggcagctc gaccgtgcgc tgagccgggt ccgggaagac gggctcgaca
      361 atctgataga ggatcgtacg acccgacaac ggaccggcgg gagcgaagct ccgcagcgac
      421 gcgagcagcg gcgtcgacgc tgcggctccc gcggcgacca cgaggatcga gtctcccgtg
      481 atcacgatcg tgtcgggtct cgcgttgacg gtgaagctta cgaaatccga aacgatattg
      541 gcgctctgcg cttgcacctg ggcagtcgac ccaccggtcc ggccggtgag tttgccgtcc
      601 ggggtgagcg tgacggtggt gtccggcgtg atccatcgga ccgtggccgc aatactgtct
      661 ccccgctcat ccagcgcgcg ggcatgcagc tggatggtct gaccgacttc gactgcggct
      721 ggccggggaa cccgaatttc gagcccggcg atgccgcttt caaccgttga aagattgctg
      781 caacctgccg ctgtgatcgt ccccgccgct acgcccgcct caaccgcagc gagccaccac
      841 ttcacagcgt ctccgatgac ggtgagccgg cgatgtcccg cagcgcggtc tgggcatggc
      901 gcacccattg gtcggcgtcg ggtcccttgg gcggccgggc gagaaactcg cgcaggtgat
      961 cggctgcttg ctgggtgtcg ccacgtttga gcaaaaggaa ggccactccg tagtgcgccc
     1021 cgcccagtgt ggggtcgata tcgagggcac gccgataata gcggatcgcc tcgtccggct
     1081 ggccggtctt ggtgagcgcg atcgccatat tcaatagaat gcggctgtcg ttgggattgt
     1141 cgcggtatgc caggcggtac gacgtcagag cggcgccaaa gtcaccctgc cgctccagcg
     1201 ccagaccttc gttgagataa tcgagccgct gcggcttgag ctgctccttg ctttcccgac
     1261 caaatactcg tcgccaaaaa ctcatcgaat cgccggcacc cgcctcaggg aagtcatttg
     1321 cgcgtggggg attatgcagg gtcgggcaaa taatagtccc gcgttgaagg ggccgcaacg
     1381 gaagccaagc tatcagctat cagcaatcag caatcagtca agccgcttgg cgaatcgcct
     1441 gggaccctta agttcaagaa gccctctgat agctgatagc cgatagctga tagctcagaa
     1501 aacatgcgcc gaactactac ccccagcccc cgcggcgttc tcgaagtcga ttggccgttc
     1561 ttcggcgagc tctgccgtgc gctggccctc cgcgtcgccc gcgagtatca gcccgaggtg
     1621 gtccttggcg ttgccaaggc tggagtgatt cccggcgtcg tggtcgcctc catcctgcaa
     1681 tgcgacttcg cgtctatggt ggtgacgcga agggagagcg acgccgatcc tattctcgtc
     1741 gccggcccgc ccgccagtat cagaggccgt cgcgtcctgg tggtcgacga gacttgcgat
     1801 acggggaata cgctcaagct cgcgctgagc gaagtgcgtg cgctcaagcc gcgcgaggtg
     1861 cgcacagcgg tatcgttcaa gaccggcgag tactcgccgg acttcgcggc cttcaccacg
     1921 gagaaattga tcatccttcc ctgggatcgc gagatcattc agggtgggga gctggtaatg
     1981 cggcccgact acgctgcctg gttagggagc tagcccagag cggttgccat ccgctccgcc
     2041 gacgcccacc gttcgtcccg gctcttcgcc agcgacctca tcacgacgtc cgccacatcg
     2101 gccggcgtct ccggccgaaa cctgcgaacg tccgggactg ggtcgttgag gtgcatttgc
     2161 agaacgacga cctcgttggg atgctgaaaa ggaggtcgtc ccgccagact ctcgaacagc
     2221 acgcaaccca cggagtagag atcggtgcgg tggtcgatat ccgtcgcggc ctgcacctgc
     2281 tcggggctca tatagctgga cgtgccgacc gcaatgccgg aacgggtgag ctgggtggtt
     2341 ccggcggcct cgatcgcccg cgcaattccg aagtcgagca gcacggcgcc ggcgtcagat
     2401 aaaactatgt tttccggctt gacatcgcgg tggataatgc cgaccgcgtg ggccgccttg
     2461 agtgcgccca gcaggtcccg agtcatgcga acagcggcgt ccacggggag ttgccgtccc
     2521 cggtccagct ggatccgcag cgttggccct tcgatgaacg gcatgacgta gtacaccagc
     2581 cagtccgctt cgcccgagtc cagcactcgt gcgaccagcg ggtggtcgat gcgcgacgcg
     2641 agccggattt cccgaaggaa ccgctcggcg gtgacgctga cctggagttc cggatggagg
     2701 accttgaggg cgaccgaacg gccgcctcgg tcgtcggcca ggaagacccg cgcggctccg
     2761 ccacgcccga tctcccggtg caccgtatac cggtccttga gggcgttccg gacgatcggg
     2821 agcaggtcgc ggcggatcgg taccccccgt ggcaggcctg ac
//
LOCUS       DASRJQ010000078         3388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_4174498, whole genome shotgun sequence.
ACCESSION   DASRJQ010000078 DASRJQ010000000
VERSION     DASRJQ010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4174498"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /gene="recF"
                     /locus_tag="VFB89_01820"
     CDS             <1..868
                     /gene="recF"
                     /locus_tag="VFB89_01820"
                     /inference="COORDINATES: protein motif:HMM:NF000822.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HZM26070.1"
                     /translation="KANRVRLNRSPLRQAREIIGLLRTVVFSPGDIAIVKGDPNERRR
                     FLDDLVVVRWPRMAGVRADYERVLRQRNTLLKSLSGRLRGGSPPADAESTLDVWDTHL
                     ARTGAELLDARLATLADLAPHVSKAYAGIAPTNNDAAAEYRASVDLSTHGAADRARLT
                     ELLIDAMTERRSEEIQRGMSLVGPHRDEIVLSLGMLPAKGYASHGESWSFALALRLGS
                     FQLLRADGVEPVLVLDDVFAELDAVRRERLASGVRVAEQVLVTAAVGEDVPELLAGQR
                     FRVAAGEVRPDA"
     gene            861..1421
                     /locus_tag="VFB89_01825"
     CDS             861..1421
                     /locus_tag="VFB89_01825"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HZM26071.1"
                     /translation="MPDEQPESSRSDQPQDNPIQNGPIQHDPTGLDLAQTVARSLGAQ
                     ARRRRRSRSPRSVSPQMSGARADDRDPKPLSDAVERLVESKGWTTEINVHTLLARWAL
                     LVGATNSAHSSPEAYADTVLTVRADSTVWATQLRYLAPQLVAMLNEQLGDGTVTRIKV
                     LGPDAPSWKKGRLSVRDGRGPRDTYG"
     gene            1661..>3388
                     /locus_tag="VFB89_01830"
     CDS             1661..>3388
                     /locus_tag="VFB89_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZM26072.1"
                     /translation="MPRSAASQGPSNSQKGTAFVNVTIHEPSEHNFGVPPASPVHEVK
                     HRRRTSDRSPAPVQHSDQPDDVTPTPHDSITMNSTSHPAASEVINNVINNIEPGEYDS
                     ESITVLEGLEAVRKRPGMYIGSTGERGLHHLVYEVVDNAVDEALAGFCDTIVVHILPE
                     GGIQVIDNGRGIPVKEHPREKIPAVTLALTVLHAGAKFGDGGYKVSGGLHGVGVSVVN
                     ALSARLVAEVKRDGYRWSQSFSYGEPDGPLERLEPSEETGTTVTFYASSDIFETTNYS
                     YDTLASRFREMAFLNKGLTITIVDERPDRRDEDGNAYADTFRYDEGLIDYVRYLTGSK
                     DPVHSSVIAVDADRPERGISLEVAMQWNNSYTESVHTFANTINTHEGGTHEEGFRAAL
                     TNTVNKWGENWGMIKKSEDRLSGDDIREGLTAIVSVKLAEPQFEGQTKTKLGNTEARS
                     FVQQVVNSKLGDWFEQNPGEGRMIIRKGQAAALARIAARKAREAARSRKGLLGGGGLP
                     GKLRDCQSTNPEECEVFIVEGDSAGGSATGGRDPRIQAILPIRGKILNVEKARIDRIL
                     QNTEVQAIIS"
BASE COUNT          645 a         1073 c         1079 g          591 t
ORIGIN      
        1 gaaagcaaac cgagtcaggc tcaaccgttc cccgctgcgt caggcccggg aaatcatcgg
       61 cttgctccgg acagtggtgt tctcaccggg agatatcgcc attgttaagg gcgatcccaa
      121 cgaacgccgc cgcttcctcg atgatcttgt cgtcgtgcgg tggcccagaa tggccggcgt
      181 acgcgcggac tacgagcggg tcttgcggca acgcaacacg ctgctgaaat ccctgagtgg
      241 tcggctccgc ggtggttcgc cgccggctga tgccgagtcg acgctcgatg tctgggatac
      301 ccacttggct cggacggggg ctgagctcct cgacgcgcga ttggccacgc ttgctgatct
      361 cgcaccccac gtcagcaagg cgtacgccgg cattgcgccc actaataatg acgccgccgc
      421 ggagtaccga gcgtctgtgg acctcagcac ccacggggca gcggatcgag caagattgac
      481 cgagctgctg attgatgcga tgacagagcg gcgctccgag gaaattcaac gcgggatgtc
      541 acttgtcggg ccgcaccgcg atgagatcgt gctctcctta ggcatgctgc ccgcgaaagg
      601 ctatgccagt catggcgagt cctggtcatt cgcacttgcc ctgcggcttg gcagcttcca
      661 gctgctccga gccgatggag tggagccggt gctggtactc gatgacgtct tcgccgagct
      721 ggatgcggta cgccgcgagc gactggcttc cggcgtacgc gtcgctgaac aggtcctcgt
      781 caccgcagcc gtcggcgaag acgttccgga actgctggcg ggccagcgct tccgggtcgc
      841 ggcgggggag gtgaggcctg atgcctgacg aacaacctga gagctcacgc agcgaccagc
      901 cccaagacaa tccaatccag aacggtccaa tccagcacga cccaacggga ttggacctcg
      961 cccagactgt cgcccgctcg ctcggcgccc aggcgcggcg ccggcgccgg tctcgatcgc
     1021 ctcggtcggt ctccccccag atgtccggcg cccgagccga cgatcgagat cccaagccgc
     1081 tttccgatgc ggtcgagcga ctggtcgaga gcaaaggttg gacgaccgag atcaacgtcc
     1141 acacactcct ggctcggtgg gcacttctgg tcggcgccac caattccgca cactcctccc
     1201 cggaggcgta cgcggacacc gttctgacgg ttcgcgccga ctcgacagta tgggcgaccc
     1261 agctgcgcta cctcgcgccg cagctggtgg cgatgctgaa tgagcaactc ggagatggca
     1321 cggtcacccg gatcaaggtc cttggccccg acgcaccctc ctggaagaag ggccggctgt
     1381 cagtccgcga tggtcgcggg cctcgtgata cctacggcta gttgtttggt gctgcgcgac
     1441 tttggcgctc atccgaggac tgaaagccga tttgtcttta agcctcgcgg cgccctcggc
     1501 atatgcgggc gcgtacaggg actcctcggg tcgctcgtat agccgcaaat ccaccgtcca
     1561 gcccttacca ggcgcatttt ttgtgttgtc cgaggtccgc tggcggaccc gctggaggac
     1621 tcctcgcgct aaactggaca aggtcacatg agcgggtcga gtgcctcgtt cggccgcctc
     1681 acaagggccg tcaaattctc agaaaggcac cgcattcgtc aacgtgacca tccatgaacc
     1741 aagcgaacac aactttggcg tgccgcctgc atcacctgtt cacgaggtga agcacaggcg
     1801 gcgcacgtct gacaggagcc ccgcgccggt gcagcattct gaccagcccg acgacgtcac
     1861 ccccaccccg catgactcaa tcacgatgaa ctccacgtcg catcctgcgg ccagtgaggt
     1921 catcaacaat gtcatcaaca atattgagcc cggtgaatac gactcagagt cgatcacggt
     1981 cctggagggc ttggaggcgg tacgcaagcg tcccggcatg tacatcggtt cgacggggga
     2041 gcgtggcctg caccaccttg tgtatgaggt ggtagacaac gcggtcgatg aggcgctcgc
     2101 agggttctgc gacacgattg tggtccacat tctccctgag ggcggcatcc aggtcatcga
     2161 caacggccgc ggtattccgg tcaaggagca tccccgggag aagattccgg cggtcacctt
     2221 ggcgctcacc gtgctccatg cgggcgccaa gtttggtgat ggcggttaca aggtctccgg
     2281 cggcttgcac ggcgttggcg tttcggtggt caatgcgctg tcggctcggc tcgttgcgga
     2341 ggtgaagcgc gacggctacc ggtggagcca gagcttcagc tacggcgaac cggacggacc
     2401 gctggagcgg ctggaaccga gtgaggaaac cggcaccacc gtcaccttct acgcgagctc
     2461 cgacatcttc gagaccacga actactccta cgacacgttg gcgtcgcggt tccgggagat
     2521 ggccttcctc aacaagggcc tgacgatcac catcgtcgac gagcggccgg accgtaggga
     2581 tgaggacggc aacgcgtatg cagacacctt ccgctacgac gagggcttga tcgactatgt
     2641 ccgctacctg accggaagca aggatccggt gcattcttcc gtgatcgccg ttgacgccga
     2701 tcggccggaa cgaggcatca gcctcgaggt cgctatgcag tggaacaact cctacaccga
     2761 gtcggtgcac accttcgcga acacgatcaa cacccacgag ggcggtaccc atgaggaggg
     2821 ctttcgcgcc gcgctcacca acaccgtgaa caagtggggt gagaactggg gaatgatcaa
     2881 gaagtcggag gaccggctgt ccggtgacga cattcgcgag ggattgaccg cgatcgtttc
     2941 ggtcaagctg gccgagccgc agttcgaggg ccagaccaag acgaagctcg gcaacaccga
     3001 ggcacgctcc tttgtgcagc aggtagtcaa ctccaagctg ggtgactggt tcgagcagaa
     3061 ccccggtgag ggcagaatga tcatccgcaa gggacaggca gcggcgctgg cccggatcgc
     3121 ggcccgtaag gcccgggaag cagcccgcag ccgtaagggt ctgctcggcg gcgggggact
     3181 gcccggcaag ttgcgcgact gccagtcgac caatcccgag gagtgcgagg tcttcatcgt
     3241 cgagggcgac tcggccggcg gttcggcgac tggcggccgc gacccgcgga tccaggcgat
     3301 cttgccgatt cgcggcaaga tcctcaacgt tgagaaggcc cgtattgatc gcatcttgca
     3361 gaacaccgag gtgcaagcga tcatctcc
//
LOCUS       DASRJQ010000079         4858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2482330, whole genome shotgun sequence.
ACCESSION   DASRJQ010000079 DASRJQ010000000
VERSION     DASRJQ010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2482330"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /gene="rpsL"
                     /locus_tag="VFB89_01835"
     CDS             complement(<1..298)
                     /gene="rpsL"
                     /locus_tag="VFB89_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HZM26073.1"
                     /translation="MPTINQLIHTGRRDVLAKDKAPALKSNPQKRGVCTRVYTTTPKK
                     PNSALRKVARVRLTNGFEVTAYIPGEGHNLQEHSIVLIRGGRVKDLPGVRYHIVR"
     gene            complement(377..760)
                     /gene="acpS"
                     /locus_tag="VFB89_01840"
     CDS             complement(377..760)
                     /gene="acpS"
                     /locus_tag="VFB89_01840"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HZM26074.1"
                     /translation="MTIVGHGVDLVEVERIRSALDRHGERFANRIFTQGEQTQAGTGP
                     LRVQFLAGRFAAKEAVMKALGTGWARGVSWTDIDVKRLPSGKPEVTLRGKCKEIADGL
                     GIGRWDISITHTDGHAAASVVATTA"
     gene            875..2131
                     /locus_tag="VFB89_01845"
     CDS             875..2131
                     /locus_tag="VFB89_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26075.1"
                     /translation="MLRLRSISVAVLAGAVALGCSDSNQPVGPSDQSDNSSSEPAMRA
                     AAQTGADDPIALAKSVPGFGGFFIDEQGTPTIYLKDTGKRAAAEQALSPWLGSHGVRG
                     AQMKVLHADFDWADLERWHAKGSPAALAVKGAVFAGADHATNRLRIGIERGASAGEVR
                     QALTRLGIPASAVTVEVTEPVVRLATLQESVRPVIAGVQINFPGFLCSIGFNATRSGT
                     SGFVTASHCTNTQGGTENTPYWQPLQSTSPIQIATEAVDPVYTSGGSCPAGRLCRSSD
                     ASFARYINNTQNTLGRIARTSSTRKQDLTIIGNWTITADAPSACIAVGTIVNKVGRTT
                     GWSQGRVTATCVDVNVGGTNITQLRQTLVQATVGAGDSGSDVFAINSGTNVTLEGVLW
                     GGSGSHTFVFSPLANVKQELGALTTH"
     gene            2182..2601
                     /locus_tag="VFB89_01850"
     CDS             2182..2601
                     /locus_tag="VFB89_01850"
                     /inference="COORDINATES: protein motif:HMM:NF024102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsuPI-related putative proteinase inhibitor"
                     /protein_id="HZM26076.1"
                     /translation="MLYSLLLAAALVTDSMTLDVSVPHNVRAGEPVALAIHLTNSGTA
                     PMTLYLRGRPAAFDLIVTDTRGKIVWRRLENAVTSMALQVRELAAGESLTFEDVWSQR
                     TNAGAVVQPGEYRVKGQLLTDTDTPLETPLRSFRITP"
     gene            2816..3700
                     /locus_tag="VFB89_01855"
     CDS             2816..3700
                     /locus_tag="VFB89_01855"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HZM26077.1"
                     /translation="MRSARSRHHTRLLALGTVPLALLALGCGSDSSTGPNSQAAHNLK
                     GLPSFSDVRTALKQVVAEQNGGLGFNMWATIIDRAGVVQVVAFSGDSPVDQWPGSRVI
                     SAQKANTANSFSLTAFALSTANLYAAVQPGGSLFGLQESNPVDPTTAYDGNIQNFGTA
                     HDPLVGNRLGGVNVFGGGLALYAPDGSLIGALGVSGDASCADHIIGWKLRHNLGLDFV
                     PAGVATGGFNANPNNQNGSTDNIIFDLTADDKSPSGFGHPVCDNPPPADVNNPPAGTM
                     TFIAQHLPQTHPIRQVVP"
     gene            3801..4739
                     /locus_tag="VFB89_01860"
     CDS             3801..4739
                     /locus_tag="VFB89_01860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZM26078.1"
                     /translation="MSEPGAAALSVRGLRKAYKDVVAVDGLDLEVRPGECFGLLGPNG
                     AGKTTTVEICEGLTSADSGDVVVLGRRWGRDDRELRQLLGISLQETQFSEKLTVLETV
                     RLFRSFYARGPEPEAVIRMVQLDEKSSGRVGQLSGGQKQRLALACALVGAPELLFLDE
                     PTTGLDPQSRRQLWELIDEFRAAGRSILLTTHYMEEAERLCDRVAVMDHGKVIALGTP
                     RELIASLEAEHVLEFAVGEGAAVDAATLRAIEGVCSAARRDGTWQLQVTELHRAMPAL
                     LAELRREGAQLTELRTHSPTLEDVFVSLTGRHLRDD"
BASE COUNT          855 a         1589 c         1504 g          910 t
ORIGIN      
        1 cgcgcacgat gtgataacgc accccaggca gatccttcac gcggccaccg cgaatgagca
       61 cgatcgagtg ttcctgcaga ttgtgccctt cgcccgggat ataggcggta acttcgaacc
      121 cgtttgtgag ccgaacacgc gccaccttac ggagggccga gttgggcttc ttgggcgtgg
      181 tggtgtacac ccgtgtgcag acgcccctct tctgggggtt gctcttgagc gcgggtgcct
      241 tgtccttggc gagcacgtcc cgccggccgg tgtggatcag ctggttgatt gtcggcatct
      301 gccctgctgt tggttcggtt gatcccggac gcgatccggg aaagaggtgc aagtgtagcg
      361 acggtgcgta ggtaggtcaa gcagtcgtgg ccaccacact ggctgcagcg tggccgtccg
      421 tgtgagtgat gctgatgtcc cagcgaccga tgccaagccc atcagctatc tccttgcatt
      481 tgccccggag tgtgacctcg ggtttaccag acgggagccg cttcacgtcg atgtcggtcc
      541 acgagacgcc tcgcgcccat cccgtgccga gggctttcat gacggcttct tttgcggcaa
      601 accggccggc cagaaactga accctgagcg gtccggtgcc agcctgcgtc tgctcacctt
      661 gggtaaagat tcgattggcg aaccgctcac cgtgacgatc gagagccgag cgaatgcgct
      721 cgacctcgac caggtcaacg ccgtggccga cgatggtcat cctggcaatc tagcgaacgg
      781 tggcctgctg gttgcacggc accaaccgac ataccttgca gcgccttcaa aatcgggtaa
      841 ttcgacgcga aaacccctgc ttcgtgaggc tagcatgttg cgtcttcgca gcatctccgt
      901 tgcggttctc gcgggcgcgg tcgctctcgg ctgttccgac agcaaccagc ccgttggccc
      961 cagcgaccaa tccgataact cttcttccga gcccgcgatg cgggctgccg cgcaaactgg
     1021 cgccgacgat cctattgcgc ttgccaagag cgttcccgga ttcggcgggt tcttcataga
     1081 cgagcagggc acacccacga tctatctcaa ggacaccggc aaacgagctg ccgccgagca
     1141 ggcgctttca ccgtggttag ggagtcatgg tgtccgggga gcccagatga aggtcctgca
     1201 cgccgacttc gattgggctg acctggagcg ctggcatgca aagggctctc cggccgccct
     1261 ggccgtgaag ggcgccgtgt ttgcgggcgc cgaccacgcg accaatcgcc tccggatcgg
     1321 aatcgagcgg ggcgcctcgg ctggcgaagt tcggcaggcg ctgacccggc tgggcatccc
     1381 tgcttcggct gtaacggtgg aggtaactga gcccgtggtt cgcctggcca cacttcagga
     1441 aagcgtccgc ccggtgatcg caggcgtgca gatcaacttc cccgggttcc tctgctcgat
     1501 cggattcaac gcgacgcgga gtggcacaag cggcttcgtc accgcgtcac actgcaccaa
     1561 cactcagggc ggcaccgaaa acactcccta ctggcagcca cttcagagca caagtcccat
     1621 ccagatcgct accgaggcgg ttgatccggt gtacaccagc ggtggctcct gtcccgccgg
     1681 acggctttgt cggagcagcg atgcctcgtt tgctcggtac ataaacaaca ctcagaacac
     1741 gttgggcagg atcgcccgca cctcgagcac caggaagcag gatcttacca tcatcgggaa
     1801 ctggaccatt acggccgatg ctccgtccgc ctgcatagcg gtcggtacga tcgtcaataa
     1861 ggtcggacgg actaccggct ggagtcaggg acgggtgacc gctacgtgcg tcgacgtcaa
     1921 cgtgggcggg accaatatca cgcagctccg tcagacgctc gttcaggcta cggttggcgc
     1981 aggcgacagc ggctccgacg tgttcgccat caacagtggg accaacgtga cgctcgaagg
     2041 cgtcctttgg ggtggctccg gaagccacac cttcgttttc agtccgttgg cgaatgtgaa
     2101 gcaggagctg ggtgccctga cgacccactg acctcatacg gccgccgggc tcgtcccggc
     2161 ggccgttgcc gagtcccgcg tgtgttgtat tcactccttc tcgccgcagc cctcgtcacc
     2221 gattccatga cgctcgatgt ttctgtgccg cacaacgtgc gagccggtga accggtcgcc
     2281 ctagccatcc atctaacgaa cagcggcacg gcgcccatga ctctgtatct gcggggacgc
     2341 cccgctgctt tcgacctgat cgttacggac actcgcggga aaatcgtctg gcgcaggctc
     2401 gagaacgctg tcacctcgat ggcgcttcag gttcgtgagc tggccgcggg tgaatcgctg
     2461 actttcgaag acgtctggtc gcaacgaacg aacgcgggcg ctgtcgtgca accgggagag
     2521 taccgggtaa agggtcagct actcacagac accgacacac cacttgagac gcctctccgc
     2581 tcgttccgca tcaccccctg actgcaagaa tttgcacctg ccgcagagcc tttcccctct
     2641 gtagcgcacc ctccatccgg cctgcatctt agcgcgggca gggagaagcc ggcggtcacc
     2701 cggctcaaac atcatcgtta caaaccagct tctcccccga atccgggggg tcgagatggt
     2761 gcacccattt tcgactgccc ggccacacgt gtttccccct acaaaggagg cagtcatgcg
     2821 tagcgctcgc tcgcggcacc acacccggtt gctcgcgctc ggtacggtcc cgctcgccct
     2881 gctcgcgctc gggtgcggct ccgacagcag caccggtccc aacagtcagg cggcgcacaa
     2941 cctcaaagga cttccgtcct ttagtgatgt ccggacggcg ctgaagcagg ttgtggccga
     3001 gcagaacggc ggcctgggat ttaacatgtg ggcgacgatc atcgatcgag cgggcgtggt
     3061 gcaggtggtc gccttcagcg gcgattcgcc agtcgatcag tggccgggaa gccgggtgat
     3121 ctcggcacag aaggcgaata cggccaattc gttcagtctg accgccttcg ctctctccac
     3181 cgccaacttg tatgccgccg tccagccggg aggcagcctc ttcgggctcc aggagagcaa
     3241 cccggtggat ccgaccaccg cctacgatgg gaacatccag aatttcggaa cagctcatga
     3301 tccgctggtg ggcaaccggc tgggcggtgt aaacgtgttc ggcggtggtc tcgcactcta
     3361 tgcgccggac ggatcattga taggcgcgct gggagtcagc ggcgatgcct catgcgccga
     3421 ccacatcatc ggatggaaac tccgtcacaa tctggggctc gacttcgtgc cggcgggcgt
     3481 tgcaaccggc ggcttcaatg ccaaccccaa caaccagaac ggtagtaccg acaatatcat
     3541 tttcgatctc acggccgatg ataagagccc gagcgggttc ggtcatcccg tttgcgacaa
     3601 ccccccaccg gcagatgtga acaatccacc cgcgggcacg atgaccttca tcgcgcagca
     3661 tctgccgcaa acccacccga ttcggcaggt cgtgccctga ctctcacgcg tacctccggg
     3721 ctctcgttct tggagcccgg aggctctgcc cgcgcacttc ccattaccgc cacgccgccc
     3781 ctcagatgta gcttcccctc atgtccgaac ccggtgctgc ggcgctttcg gtccgcggcc
     3841 ttcgtaaagc gtacaaggac gttgtggcag ttgatggcct cgacctcgag gttcgcccgg
     3901 gcgaatgctt cggcctgctc ggccccaacg gggcaggcaa gaccacgacg gtcgagatct
     3961 gcgagggctt gacgagtgcg gacagcggcg acgtcgtcgt gctgggccgc cgctggggcc
     4021 gagacgatcg ggaactgagg cagttgctcg ggatctccct tcaggaaact caattttccg
     4081 aaaagctcac cgtgctggag acggtgcggc tctttcgcag cttctatgct cgaggcccgg
     4141 agccggaagc cgtcatccgc atggttcagc tcgacgaaaa gtcgagcggc cgagtgggcc
     4201 agctctccgg cgggcagaag cagcgcctcg ccctggcatg cgcgttggtc ggcgcgcccg
     4261 agctgctctt cctcgatgag ccaaccacgg ggctcgatcc gcagtcgcgc cgccagctct
     4321 gggagctgat cgacgagttc cgcgcggcgg gccgttccat cctgctgacc acccactata
     4381 tggaagaggc ggagcggctc tgcgaccgtg tcgcggtcat ggaccacggc aaggtcatcg
     4441 ctctgggcac tcctcgcgag ctgattgcgt cgctcgaggc tgagcacgtg ctcgagtttg
     4501 ccgtgggcga aggcgctgca gtcgacgccg ccacactgcg tgcgatcgag ggcgtctgct
     4561 ccgccgccag gcgagacggc acctggcagc tgcaggtgac agagctgcac cgggccatgc
     4621 cggcccttct cgcagagctc cggcgcgaag gagctcagct gaccgagctg cggacccatt
     4681 cgccgactct ggaggacgtt ttcgtgtcgc tgaccggccg gcacttgaga gatgactgac
     4741 gtcgccggcc acccgccgct gactggccgg cacagacacc cgctggcgca gctcactctg
     4801 gtccggtacc tcgagttcgt tcgagagccg gaagcggtct tctgggtgtt catcttcc
//
LOCUS       DASRJQ010000080         8342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_790374, whole genome shotgun sequence.
ACCESSION   DASRJQ010000080 DASRJQ010000000
VERSION     DASRJQ010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 8342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8342
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790374"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2200
                     /gene="glgP"
                     /locus_tag="VFB89_01865"
     CDS             <1..2200
                     /gene="glgP"
                     /locus_tag="VFB89_01865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HZM26079.1"
                     /translation="PPFTVHACLNYLPLPMLTQQSRIPYLPARIEGLASIAMNLWWSW
                     SREARSLFRSIDAPLWHQVRHNPLDFLCQVDPARVAAAASQSDFLRQYDEVMARMANA
                     LTSTDTWFNRHNPEYDDGPVAYFCAEFGLHASVPIYSGGLGVLAGDHCKSASDLGVPM
                     VGVGLFYMKGYFDQRLRLDGWQQDSDEEYDISRTPLEAVLGPDHEPILATVETAGRPV
                     HVRAWRVMVGRIPVYLLDTNVEENDPADRGLMNKLYAGGPELRLRQEWILGAGGVRVL
                     RALGIHPSVWHANEGHAAFMMVERIRESTAQGMPFPDALRAVRSRSIFTTHTPVPAGH
                     DAFDVDEVAECTGPIWEQMGIDRETLLHLGQHPALPDQFHMTATSLRCASRVNGVSRQ
                     HGQLSRHLWRDFWPGRPWETVPIRHVTNGVHLRTWMAAQIMDLLDQHLGPYWLDRKDD
                     PSLWDQVLTLNREELWRVHLELKGSLLDYIREDARHRFAGQHKEAAQVVGAGTLLDPK
                     ALTIGFGRRFATYKRANLVFRDLARLRRLLTDARRPVQIVFAGKAHPADNPGKEVLQS
                     VYQFTRDPAFEGRVAFLEDYDMHLAHLLVQGTDLWLNLPRPPLEASGTSGMKAALNGV
                     PQLGTLDGWWKEGYDGLAGWAIQPSAENEDGDAADAERFYRLLEEQVVPLYYNRGAHG
                     IPLGWVEKMRYSIRLAGTRFTARRMMQEYVQEYYAPAARGESSADDPPAI"
     gene            2133..3707
                     /locus_tag="VFB89_01870"
     CDS             2133..3707
                     /locus_tag="VFB89_01870"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HZM26080.1"
                     /translation="MSRSTMRQQLEGSPLPTILPRSEARRLPERGVAAPILTPRGEAV
                     SIVHLSAEYAPYARTGGLAEAIMGLASFQHARGLPVAVLMPLYRTVRDEAPDLEPVGP
                     PFRVPLGARTEEARLFRIAGTPPDGPRMYFLEHIEFFNRPGIYGDPGGDYPDNARRFA
                     FFALASCLALPRVAPGPVILHAHDWHSALAPAYLRTVLASEAWVRDALAIISVHNPGY
                     QGQFGPEALLELGLPVEIYNWQQLEWYGKVNFLKGGLAFADWALTVSPQQASELRTPG
                     GGFGLHDMFIALGSRLVGILNGIDQQVWDPATDSQILAPYTADHLQGKRRCKAGLQLS
                     YGFPERSDVPLFGLTGRLVQQKGLDLILASHRLLQQDAQFVFLGSGDPHYEQALVALA
                     AAAPDRIGVQLDFRDVLEHRLMAGVDLFLMPSLYEPCGLTQMRAQRYGSLPIGRRVGG
                     LADTIEDGVSGFLFDAYHAMALEDAAFRAISTYARPELWTQMMRSAMARDFSWERSAG
                     AYLEVYRRVVAAGPRA"
     gene            3720..5408
                     /locus_tag="VFB89_01875"
     CDS             3720..5408
                     /locus_tag="VFB89_01875"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein
                     domain-containing protein"
                     /protein_id="HZM26081.1"
                     /translation="MDFVLTLHSHLPWVLNHGRWPHGSDWLCEAAVDTYLPLLESFQA
                     LERESVAAPVTIGVTPVLANQLASPVFVEELEAFFEQRLEACDAADVELRKTDPEAAP
                     LVDFWRQRYLRLRHLFRLVDEDLIAALRALEQKGRIEIIGSAATHGFLPLLARDESIR
                     LQLAVGVAEHRRLFGRLPKGCWLPECAYRPRGAWAPWPTAPRSGVRRGIEEHLADAGF
                     DFFFIDAHLAAAGRPLGVYGDPLVGPREVPDSIEPDRLQALRSPDRAYRVGPNRSGRA
                     VAAFVRNPRASMQVWSRHHGYPGDGQYLEFHKIRWPGGLKLWKVTGENVDLGRKGVYD
                     PRAARDQARGHGAHFAWLLGQLSVTESPGPEGVIVAPFDTELFGHWWFEGPDFVGDVY
                     RELSAQRSVRATTGSGHLGAHPARAAIRLPEGTWGANGDFSMWLGPDTAWTWERMWPL
                     EQAFWDVAGDALSRDSARPVLAQAARSLLLTQSSDWQFIISTGAVVDYAVRRFTQHCI
                     ETERLVAALTPGSGTSLEEGQALAAQLERRDHLFPDVLPAVSAALSGSRSLVLA"
     gene            5412..7349
                     /locus_tag="VFB89_01880"
     CDS             5412..7349
                     /locus_tag="VFB89_01880"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HZM26082.1"
                     /translation="MPPVRFVFGLHLHQPVGNFDHVFQQHLDDVYSPLLDRLTAGEFL
                     PVVLHVSGPLLDWLDAHAPVWMDRLGRLAADGKVELLLSGFYEPILASLPRRDRLDQI
                     GWMRDALRRRFGVEATGLWLTERVWQPELAADLAEAGVKYALVDDRHFVVCGFPQDRL
                     HRPYWTEADGKRIALFPIDEKLRYLIPFRPPEETAEYLERLGAEGQQLAVLADDGEKF
                     GGWPGTKDWVYNQGWLDRFIQSVRELAGRGLVRLSTLAQSLADVPSGGVAYLPTASYR
                     EMEGWSLPPDAALRLGRLERDLGEARMAGPDGALVRGTHWSNFLSKYPEANRMHKKMQ
                     ALSELCRARHDPPEARRAIGRAQCNDAYWHGVFGGLYLPHLREAIWRNLAAAEAQLRE
                     RESLSWEVLDLDADGHDEIWVHSSQCSVVVSPWRGGAIEELTRFNTGVNYAGVLTRRL
                     EAYHVTALGGSGDAAHGDGGAPSIHDLETALRLDERPALDADDRAIAVDRLLPADLAK
                     EAYARGDYPVVRSWASTPAHFQISQSQGIVEIRLRLHAADGPPLLKTIRIGADGKVDL
                     AYEWTGGHDHGDALFAPELSLFRPLSIQYAPDPDVWSFPIETVAKSEKGLDRTHQGES
                     ITPRWPFHLGAVRLELGPLPD"
     gene            7371..8255
                     /locus_tag="VFB89_01885"
     CDS             7371..8255
                     /locus_tag="VFB89_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HZM26083.1"
                     /translation="MPTSPSSVTLGLVQSRCTLDPEQNFRQALAAIREARAKGAQIVC
                     LPELFRSQYFCQTEDHRYFDLAETIPGPSTEALSNLAAELGVVIIASLFERRAEGLYH
                     NTAAVIDADGRYLGKYRKMHIPDDPQYYEKFYFTPGDLGFRAWDTKFGRIGVLICWDQ
                     WYPEAARLTALTGAQILFYPTAIGWLPAEKAEHGTQQQAAWETIQRSHAIANGVYVCA
                     VNRVGHEGDPSGGLEFWGGSFVTDPGGRILVKGGHGEEVLVASCDLSKVDASRTHWPF
                     LRDRRIDAYGDLTKRYLD"
BASE COUNT         1377 a         2710 c         2712 g         1543 t
ORIGIN      
        1 acccccgttc actgttcacg cctgtctgaa ttaccttccc ctgcctatgc tgacccagca
       61 atcccggatc ccctatctcc ccgcccgcat cgaggggctc gcctccatcg ccatgaacct
      121 gtggtggagt tggagtcgcg aggcgcgctc gctgtttcgc tcgatcgatg cacctctctg
      181 gcatcaagta cgtcacaatc cgctcgattt tctctgccag gtcgatcctg ctcgcgtggc
      241 ggcggcggcg agccagagcg atttcctgcg ccagtacgac gaagtgatgg ctcgcatggc
      301 gaatgcgttg accagcacgg atacctggtt caaccgacac aatcccgagt acgatgacgg
      361 gcccgtggcg tacttctgtg ccgagttcgg gctccatgcg tcggtgccga tctattccgg
      421 cggcctgggc gtgcttgcgg gagatcattg caagtctgcg tcggacctcg gtgtgcccat
      481 ggtgggcgta ggactgttct acatgaaggg atacttcgac caacgtctac ggttggacgg
      541 ctggcagcag gacagcgacg aagagtacga catcagccgc accccgctcg aggcggtcct
      601 gggtcccgat cacgaaccga ttctcgccac ggtggagacc gcgggcaggc cggtccacgt
      661 gcgtgcctgg cgagttatgg tcggccggat tccggtctat ctgctggaca ccaacgtcga
      721 ggagaacgat ccggcagacc ggggcctcat gaacaagctc tatgccggcg gcccggagct
      781 ccgactccgt caggagtgga ttctgggcgc cggaggcgtt cgggtcctgc gcgcgctcgg
      841 cattcatcct tctgtgtggc atgccaacga aggccatgca gcgttcatga tggtcgagcg
      901 gattcgggag tccaccgccc aggggatgcc cttcccggat gcacttcgcg ccgtgcgatc
      961 ccgcagcatt ttcacgaccc acacgccggt acccgcgggg cacgatgcct tcgacgtcga
     1021 tgaggtcgcc gaatgcaccg gtccgatctg ggagcagatg ggcatcgatc gagaaacgct
     1081 cctgcacctg gggcagcatc ctgcgttacc cgatcagttc cacatgaccg caacgtcgct
     1141 ccgttgcgct tcccgcgtga acggcgtttc ccgccagcac ggccaactgt cgcgtcatct
     1201 gtggcgggat ttctggcccg ggcgtccctg ggaaaccgtt cctatccgcc acgtgacgaa
     1261 cggcgtgcac ctgcgtactt ggatggcggc gcagatcatg gatctgctcg atcagcacct
     1321 ggggccctat tggctggacc gtaaggacga tccgtcgctc tgggatcagg tgctgaccct
     1381 gaatcgggaa gagctgtggc gggtccacct cgagctcaaa ggctcgctgc tcgactatat
     1441 ccgcgaggac gctcgccacc gcttcgctgg ccagcacaag gaggccgcgc aggttgtcgg
     1501 cgctggcacg ctgctcgatc ccaaggcact cacgatcggt ttcggccggc gatttgccac
     1561 atacaagcgc gccaaccttg tgtttcgcga cctggctcga ctgcgccggc tgctaaccga
     1621 cgctcggcgc ccggtacaaa tcgtctttgc gggcaaggcc cacccggcag acaacccggg
     1681 caaggaagtg cttcagagcg tttaccagtt cactcgtgat cccgccttcg aggggcgcgt
     1741 cgcgttcctg gaggactacg acatgcacct ggcccatttg ctggtccagg gcacggacct
     1801 gtggctcaat cttccccgtc cccctctcga ggcctcaggc acaagcggca tgaaagcggc
     1861 cttgaatggc gtgcctcagc taggcacgct ggacggctgg tggaaagagg gctacgacgg
     1921 cctggccggc tgggcgattc agcccagcgc ggagaacgag gatggcgacg ccgcggacgc
     1981 cgagcggttc taccggctgc tggaagaaca agtggtccca ctctactaca accgcggcgc
     2041 ccatggcatc ccgttgggct gggtggagaa gatgcgctat tcgatccgac tcgcgggaac
     2101 ccgcttcacg gctcggcgca tgatgcagga gtatgtccag gagtactatg cgccagcagc
     2161 tcgaggggag tcctctgccg acgatcctcc cgcgatctga agccaggcgg ctgccggaaa
     2221 gaggcgtcgc cgcgccgatt ctgacgcctc gaggcgaggc ggtctccatt gtccatctga
     2281 gcgcggagta cgctccgtac gcgcgaacgg gaggactggc cgaagccatt atgggcctcg
     2341 cctccttcca gcacgcgcgc ggcttgcccg tcgcggtcct catgccgctg tatcggacgg
     2401 tacgcgacga ggctcccgac ctcgagccgg tcggcccccc gttccgcgtg ccgctcggcg
     2461 cccgcaccga agaggctcgt ctctttcgga ttgccggcac accgcccgac ggtccccgaa
     2521 tgtactttct cgagcacatc gaatttttca atcggccagg gatctacggc gaccccggag
     2581 gagactaccc ggacaacgca cgccgattcg cattctttgc cctggcatcc tgtctcgcgc
     2641 ttccccgagt ggcgcccggg cccgtcatcc tccacgcgca cgattggcac agcgcccttg
     2701 ccccggccta tctcagaacc gtcctggcct ctgaagcgtg ggtgcgcgat gcgctcgcca
     2761 tcatctcggt ccacaatccg ggataccagg gtcagttcgg accggaagcg ttgttggagc
     2821 tgggcttgcc ggtcgagatc tacaactggc agcagctcga atggtacgga aaggtcaact
     2881 tcctgaaggg cggactcgcc ttcgcggact gggcgctcac cgtcagtcct cagcaagcca
     2941 gcgagttgag aactccaggc ggcggcttcg ggttacacga catgttcatc gcgctgggga
     3001 gccggctggt cggtatcctg aacggaatcg accagcaggt ctgggacccg gccacggact
     3061 cgcagatcct ggcgccgtac acggccgatc acctgcaggg caagcgccgc tgcaaggcgg
     3121 gcctccagtt gtcctacggc tttcccgagc gatccgacgt gccgctgttc ggcctgaccg
     3181 gtcggctggt gcagcagaaa ggactggacc tgatcctcgc gagccatcgt ctgctgcagc
     3241 aggacgccca attcgtcttt ctgggaagcg gcgatcccca ctacgagcag gcattggtgg
     3301 cactggccgc cgccgctccc gaccgcatcg gggtccagct cgattttcgt gacgtgctcg
     3361 aacaccgcct catggccggg gtggacctgt tcctgatgcc gtccttgtac gagccttgtg
     3421 gacttaccca gatgagggca cagcgctacg gttcactccc catcggccgg cgggtaggtg
     3481 ggctggccga cacgatcgaa gacggggtga gcggttttct gttcgatgcg taccacgcca
     3541 tggcgttgga agacgctgcg ttcagagcaa tttcgactta cgcccggccg gaattgtgga
     3601 cccagatgat gcgctcggcg atggctcgcg actttagttg ggagcgctca gccggcgcct
     3661 atctcgaagt gtaccggcgt gtcgtggcgg cgggcccgcg cgcctgatcg gaccattcga
     3721 tggacttcgt tctgacgctg cacagccact tgccctgggt actcaaccat ggccggtggc
     3781 cccacggtag cgactggctc tgcgaagcgg cggtggatac atacctgccg ctgctcgaat
     3841 ccttccaggc cctggaacgg gaaagcgtcg cggccccggt gacgatcggt gtgacaccgg
     3901 tcctcgcgaa tcagctggcg agccccgtgt tcgtggagga gctggaggcg tttttcgagc
     3961 aacggttgga ggcgtgcgac gccgccgacg tcgagttgcg caagaccgac ccggaagcgg
     4021 cgccgctggt cgatttctgg cgacagcgat acctccggct gcgccatctc ttccgcctcg
     4081 tagacgagga tctgattgcc gcgcttcgtg ccctcgagca gaagggtcga atcgaaatca
     4141 tcggctccgc cgccacccac ggcttcctcc cccttctggc gcgcgacgaa agtatccggc
     4201 tccagctcgc ggtgggtgtg gccgaacacc gtcgcttatt cggccggctt ccgaagggat
     4261 gctggcttcc cgagtgcgcc tatcggccgc ggggagcctg ggccccctgg cctacggcac
     4321 cgaggagcgg cgttcgccgc ggcattgaag aacatctcgc tgatgcgggc ttcgacttct
     4381 tcttcatcga tgcccatctc gcagccgccg gccgaccgct gggagtgtat ggtgatcctc
     4441 tggtcggccc gcgggaggtc cccgacagca tcgagccgga tcgcctgcag gcattgcgat
     4501 cccccgaccg cgcctaccgc gtcggcccga atcgcagcgg ccgggcggtc gcggcgttcg
     4561 tgcgcaatcc gcgagcttca atgcaggtct ggagccggca ccacggttat cccggcgacg
     4621 gacagtacct cgagttccac aagattcgct ggcccggcgg gctcaagctt tggaaggtca
     4681 ccggcgagaa cgtcgatctg ggcagaaagg gcgtctacga cccacgggct gcgcgagacc
     4741 aggcacgggg tcacggcgcg catttcgcct ggctgcttgg ccagctctca gtcaccgaat
     4801 ctcccgggcc ggaaggcgtg atcgtggcgc cgttcgatac cgaactgttc ggccactggt
     4861 ggttcgaagg acctgatttc gtcggcgatg tctatcggga gctttctgcg cagcggagcg
     4921 ttcgagcgac aacgggctcg ggtcatctcg gcgcccatcc ggctcgtgcg gcgatccggc
     4981 ttcccgaggg gacctggggt gccaacggcg acttctccat gtggttgggc ccggacacgg
     5041 cttggacctg ggagcggatg tggcccctgg agcaggcctt ctgggacgtc gcaggcgatg
     5101 cgctgagccg ggactcagct cgccccgtgc tggcgcaggc agcgcgctcg ctgctgctga
     5161 cccaatcgtc cgattggcag ttcatcatct ccaccggcgc cgtggttgac tacgccgtgc
     5221 gccggttcac tcagcattgc atcgagaccg agcgactggt cgccgcgctc acgcccggca
     5281 gcgggacgtc cctcgaggag gggcaggcgc tcgccgccca gctcgagcgg cgcgatcacc
     5341 tcttcccgga tgtcctccct gcggtgagcg ctgccttgag cgggtcgcgc tccctggtcc
     5401 tggcctgagt catgccgccc gtacgcttcg tcttcgggct gcacctccat cagccggtcg
     5461 gcaatttcga ccacgttttc cagcagcatc tcgacgatgt ctactccccg ctgctcgacc
     5521 gcctgacagc gggcgagttc ttgccggtcg tgcttcatgt gtccggcccg ctgctcgact
     5581 ggctcgacgc ccacgctccc gtttggatgg accgcctggg gcgactggct gccgacggca
     5641 aggtggagct cctgctctcg ggattctacg agcccattct ggcctcgctc ccgcgacgcg
     5701 atcgacttga ccagatcgga tggatgcggg acgcacttcg acgccggttc ggcgtcgagg
     5761 caacaggtct ctggctcacc gaacgcgtct ggcagccgga actcgccgcg gacctcgccg
     5821 aagccggcgt gaagtacgcc ctggtggacg accggcattt cgtggtctgc gggtttccgc
     5881 aggatcgtct ccatagaccg tactggacgg aggcggacgg caagcggatc gcgctctttc
     5941 ccatcgacga gaaactgcgg tatctgatcc cgttccggcc gccggaagag acagcggagt
     6001 atctcgagcg gctcggagcg gaagggcagc agctcgcggt cctggccgat gatggagaaa
     6061 agttcggcgg ctggccggga accaaggact gggtctataa ccagggctgg ctcgaccggt
     6121 tcattcagtc cgttcgagag ctggccgggc gtggccttgt tcgcctcagc acgctggctc
     6181 agtccctcgc cgacgtgccg agtggaggag tcgcctatct cccgaccgca tcgtatcgtg
     6241 agatggaggg gtggtcgctg ccgccggacg cggccttacg gctcggccgg ctggagcggg
     6301 acctgggtga ggctcgcatg gcgggtccgg acggggcgct ggtccgcggc acccattggt
     6361 ccaacttcct gtcgaagtat cccgaagcaa accggatgca caagaagatg caggcgcttt
     6421 cggagctgtg ccgggcgcgg cacgatcctc ctgaggcgcg tcgggccatc ggacgggccc
     6481 agtgcaacga cgcgtattgg cacggggtgt tcggggggct ctatctcccc catctgcgcg
     6541 aggcgatctg gcgtaacctc gccgctgccg aggcgcagct cagggaacgc gagagcctga
     6601 gctgggaggt gctggacctc gatgccgacg gacacgacga gatctgggtt cactcatccc
     6661 agtgctcggt ggtagtgagc ccctggcgtg gtggcgcaat cgaggagctc acccgattca
     6721 ataccggcgt gaactacgcc ggggtcctca cccggcggct cgaggcgtac catgtgacgg
     6781 ctctgggcgg cagcggcgat gcggctcatg gggacggcgg ggctccaagc attcacgatc
     6841 tcgagacggc actgcgtctc gacgagcgcc cagcgctcga cgccgacgac cgcgcgatcg
     6901 cggtggatcg gcttcttccg gccgatctgg caaaggaggc gtatgcgcgc ggagattacc
     6961 ccgtcgtccg atcctgggcc agcacgcccg cgcattttca gatcagccaa tcgcagggga
     7021 ttgtggaaat tcgcctgagg ctgcatgccg ccgatgggcc tccgttgctc aagaccatac
     7081 ggattggtgc agacgggaaa gtggaccttg cgtacgagtg gaccgggggc cacgaccacg
     7141 gcgacgcgct gttcgctccc gagctgtcgc tctttcggcc gttgagcatt cagtatgctc
     7201 ccgatccgga cgtctggtct tttccgatcg agactgtagc aaaatcggaa aagggattgg
     7261 atcggacaca ccagggcgaa tccatcactc cccgctggcc gttccatctc ggcgccgtcc
     7321 ggctcgagct cggcccgctg ccggactaac tcctcgctat ctttgggccc atgccgactt
     7381 ctccgtcatc ggtaacgttg ggcctggtgc agagccgctg tactctcgat cccgagcaga
     7441 actttcgtca ggcgctcgcc gcgatacggg aagcgcgggc caaaggtgcg cagattgtct
     7501 gtctgccgga gctattccgc agtcaatact tctgccagac ggaagaccat cgatacttcg
     7561 acctggccga gacgatcccg ggcccaagta ctgaagcgct atcgaacctc gccgcggagc
     7621 tgggagtcgt gatcattgcc tcgctcttcg agcgaagggc ggagggactg tatcacaaca
     7681 cggcagcagt tatcgatgcg gacggccggt acctggggaa gtaccggaag atgcacatcc
     7741 cggatgatcc ccagtactac gaaaaattct acttcacgcc gggcgatctt ggcttccgtg
     7801 catgggacac caaattcggc cgaatcggcg tcctgatctg ctgggaccag tggtatcccg
     7861 aggccgcacg gcttacagcg ctcaccggcg cccagatcct gttttatccc accgcgatcg
     7921 gttggctgcc ggcagagaaa gccgagcatg ggacgcagca gcaggcggca tgggaaacaa
     7981 tccagcggag ccacgccatc gcgaacggag tctacgtgtg tgcggtcaac cgcgtgggcc
     8041 acgagggtga cccgtccggg gggctcgaat tctggggcgg aagcttcgtc accgacccgg
     8101 gcggccgcat tctggtcaag ggcggtcacg gcgaggaggt tctggttgcc agttgcgatc
     8161 tgtccaaggt cgacgcgagc cggacccact ggcccttcct ccgcgaccgg cggatcgatg
     8221 cgtacggcga tctgaccaaa cgatacctag actgagactg cgtgaacggt gaacggtaag
     8281 cagcaaacgg ggggtctcgg tgggccggac gccccccgtt caccgtttac cgttcacgcc
     8341 tt
//
LOCUS       DASRJQ010000081         3096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1913658, whole genome shotgun sequence.
ACCESSION   DASRJQ010000081 DASRJQ010000000
VERSION     DASRJQ010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1913658"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="VFB89_01890"
     CDS             complement(<1..1033)
                     /locus_tag="VFB89_01890"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZM26084.1"
                     /translation="MELLEKLAAALGDRYRVERELGHGGMALVFLAEDLKHRRRVAIK
                     LLKPDLSAMLGGERFLREIEIAATLQHPHILPLYDSGQASGLLYYVMPFVEGESLRER
                     LTREEQLPIDAALRVTREVGSALQYAHEHGVIHRDIKPENIMLSAGHAVVADFGIARA
                     LDAAGVEQLTQSGLVIGTPQYMSPEQAAGEKVDARSDQYSLACTLYEMLIGEPPFTGP
                     SSQAVIARHSLEPVPHIRVVRQTVPLPVECAVTQAMAKLPADRFPSVQQFVDALESPQ
                     PASVPTQVLRPSSSRPASSLGRQVLVGLAAAVVVMAATWWAVAGRTGSRDHPPLSRVT
                     AVAVLPFQDL"
     gene            1319..1921
                     /locus_tag="VFB89_01895"
     CDS             1319..1921
                     /locus_tag="VFB89_01895"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZM26085.1"
                     /translation="MARLLGLGERFPEFSMHACVSAEPGSEFRIVDSHSLEGKWAAIF
                     FWPLDFTFVCPTELVEFDRALPQFRALNTRVFGVSGDSHYAHLAWRKESAALRNLGFP
                     MLSDYKKELAEELGILDPQEKVPVRATYILDPEKTIRWLCVNDLSVGRNTDEVLRVLE
                     ALQSGELCPANWASGKPTLERPTDIDRAVRWETSEHFALR"
     gene            2014..2637
                     /locus_tag="VFB89_01900"
     CDS             2014..2637
                     /locus_tag="VFB89_01900"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZM26086.1"
                     /translation="MNHRVARYNSRRALLDEALATIRAEGVEGLTLREIGARLGVSRT
                     ALYRHFADKRALLTAVATEGFLTLRQQLLAAWEEGGRGDAAFYAMGVAYVRFAVANPS
                     YYRVMFGGFVDPEGRDPELVAEAQGAFQALVDALVTLQHEAVFQHDDPVLMARYVWAL
                     VHGVALLGIDGQLREPGGVEELMRYAFERLRTGIRAKPAHRRRSPRS"
     gene            2706..>3096
                     /locus_tag="VFB89_01905"
     CDS             2706..>3096
                     /locus_tag="VFB89_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26087.1"
                     /translation="MWRTIILSVLFSAVSWTVSAASPIHRPAAIILLRHADKPEDPGD
                     VHLSQAGVKRAEVLVSFITTDTAMTRLGLPVAIFATKTAKDGNGQRTQETVAPLSRRL
                     GVPVQTPFRTKDYGSLAKLIFGNTAYGG"
BASE COUNT          537 a          962 c         1038 g          559 t
ORIGIN      
        1 cgaggtcctg gaaaggcagc acagcgaccg cggtgaccct gctgagtgga gggtggtcgc
       61 gactccctgt acgtcctgcg acggcccacc aggtggcagc catgactacc acagccgccg
      121 cgagcccgac cagcacttgc cggccgaggc tggacgcggg ccggcttgaa gacggcctga
      181 gtacctgcgt tgggactgac gcgggctgcg gcgattcgag cgcatcgacg aactgctgca
      241 cagagggaaa gcgatcggcg ggaagctttg ccatcgcctg cgtgacagca cactccaccg
      301 gcagtggcac ggtctgacga actacccgga tgtgcggcac gggctcgagc gagtgccggg
      361 cgatgaccgc ctgagacgat ggcccggtga agggcggctc accgatcagc atctcgtaca
      421 gggtgcacgc cagactgtac tggtcgcttc gggcgtccac cttctcacca gcggcttgct
      481 cggggctcat gtattgtggt gtcccgatca ccaggccgct ttgcgtgagc tgctccacgc
      541 cggccgcgtc cagtgcccgc gcgatgccga agtcggcgac gacggcatgg ccggcggaca
      601 acatgatgtt ctccggcttg atgtcccggt ggatcacgcc gtgctcgtga gcatactgta
      661 gagcactgcc gacttcgcga gtgacccgga gagctgcgtc gatcggaagc tgctcttctc
      721 gagtgagccg ctcccggagc gactcacctt cgacgaacgg catcacgtag tagagcagtc
      781 cggaggcctg gcccgagtcg tacaacggga gaatgtgggg gtgctggagg gtcgcggcga
      841 tttcgatctc gcggaggaac cgctcgccgc cgagcatggc cgacaggtcg ggtttgagca
      901 acttgatggc gacgcgtcgg cggtgcttca ggtcctcggc gagaaagacg agcgccatcc
      961 cgccgtggcc cagttcccgc tcgacccgat atcggtcgcc gagtgcggcc gcaagcttct
     1021 cgaggagttc catgacccca ctctttccgc ggtggggaga atgtgccccc agtcgggggt
     1081 gcaggcaagg aagcgccctc gaagggctcg aacgcgcgac ctcaggtaac tagggtcgcg
     1141 cgtgtgtcta actgcagggt gagtgggtgc agcgtcgccg ccggtcttga cggtgctggt
     1201 accgcaccgt acgctggctc ctctatagag gatttgcctc aaaagggcgc cccggtgatt
     1261 ggattagcgt tgccaccggg cggcgtgacc ctcccacctt gtccgattgg aggtccctat
     1321 ggcacgacta cttggcctag gggagcgctt tccagaattc tcgatgcacg cgtgcgtctc
     1381 agccgagcca ggatcggaat tccgaatcgt cgattcgcac agcttggagg gaaagtgggc
     1441 tgcaatcttc ttctggcccc tcgacttcac gttcgtgtgc cccaccgagc tcgtggagtt
     1501 cgaccgcgcg ctcccacagt ttcgcgccct caacaccagg gtgttcggcg tgagtgggga
     1561 cagccactac gctcaccttg cgtggcgcaa agagagtgcg gcccttcgca atctcggatt
     1621 cccgatgctg tcagactata agaaggagct ggccgaagag ctggggattc tggatccgca
     1681 ggagaaggtg cccgtccggg cgacctacat tctggacccg gagaaaacga tccggtggct
     1741 ctgcgtgaac gacctgtccg tgggccggaa caccgacgaa gtgttgcggg tgctcgaggc
     1801 gctccagagc ggggagttgt gcccggccaa ctgggcctca gggaagccaa cgctcgagcg
     1861 accgacggac atcgacaggg ccgtgcggtg ggagacctcg gagcacttcg ctctccgctg
     1921 actccggcct ggttggagcc caggaggcaa tcaagcccga cagcgggtta gcgttgtcgg
     1981 gtcacatgcg atgtcggcgc tgacctttca cctgtgaacc atcgggtcgc caggtataac
     2041 agccgccgag ctctcctgga cgaggcgctc gccacgatca gggccgaggg cgtggaaggg
     2101 ttgaccctcc gggaaatcgg cgcccgcctg ggcgtgtctc gaacggcgct ctaccgacac
     2161 ttcgccgaca agcgtgcgtt gctcacggcc gtcgctaccg aaggcttcct cacgctgcgg
     2221 cagcagctgc ttgcggcgtg ggaggaaggt ggccggggtg atgcggcgtt ttacgcgatg
     2281 ggcgttgcct atgttcgctt cgccgtggcg aatccgtcct actatcgcgt gatgttcggc
     2341 gggtttgtcg acccggaagg ccgggatccc gagctggtgg ctgaagcgca gggtgctttc
     2401 caggcgctgg tggacgctct cgttacgttg cagcacgagg cagtgttcca gcacgacgat
     2461 cccgtactaa tggcgaggta cgtatgggcg ctggtgcacg gagtggcact gcttggcatc
     2521 gacggccagc tgcgcgaacc gggtggcgtc gaggagctga tgcggtatgc gttcgagcgg
     2581 ctgcgtactg gaatccgggc caagcccgct caccgccggc gctcaccacg cagttagccg
     2641 cagctgtcga gtggcaaagc gatccgccgt gaactcgcct ccagacgctt gacctcggag
     2701 tcccaatgtg gcgcaccatc atcctcagtg ttctcttcag cgccgtatca tggacggtat
     2761 cggccgcttc accgattcac cgccccgcgg cgatcattct tcttcgacat gccgacaaac
     2821 cggaggaccc cggcgacgtc catctatcgc aagcgggggt aaagcgcgcc gaagtgctcg
     2881 tctcgttcat taccaccgac acggcgatga cgaggctcgg gctgccggtg gcgattttcg
     2941 caaccaagac cgcgaaggac ggcaatggcc agcgcacgca ggaaaccgtc gcgccattat
     3001 cccgccggct cggcgtcccc gtgcagactc ctttccgcac gaaggactat ggctccctgg
     3061 caaagctgat tttcgggaat accgcgtacg ggggaa
//
LOCUS       DASRJQ010000082         2918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2481775, whole genome shotgun sequence.
ACCESSION   DASRJQ010000082 DASRJQ010000000
VERSION     DASRJQ010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2481775"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2879)
                     /locus_tag="VFB89_01910"
     CDS             complement(<1..2879)
                     /locus_tag="VFB89_01910"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HZM26088.1"
                     /translation="MLAKMAAIKTITLTLLLWSAPLLALDPSLDVSQHGHTAWTARNG
                     FSAGAIFAMAQTPDGYLWLGSEFGLFRFDGVQAVPWHPPAGQKLPERPYALLVTRDGT
                     LWIGTFAGLVSWNGDKLTSYPEIGDRFVTSLLEDREGTVWAGLLGSTSDAPTGQLCAI
                     RSGHAQCDLQGGAFGTFVWSLFEDSAGSLWAGAESGVWRWKPGSPRRYEAPGMRIGDL
                     TRTDDGHVLIGVSGGGLLQIAGDRLESHPIRDATNRDRLLPDRNVDSNKLLRDRDGGL
                     WIGTRQRGLVHVHNGRTDIFTKADGLSGDISCSLFEDREGNIWVATTGGLDRFRELPV
                     TTLSAKQGLSSDAALSVLAATDGSIWVAAEDGLTRWKNGQATVLDTASGLPHDRAQSL
                     VQDDRGRLWVFTNGGLAYLTNDDRFIGVPGVPSKEVFSMKGDGAGGLWLSTNKSLLHM
                     RDGRLVETLDWPVMGRHQQAKAVVPDQGGVWLAFWNGGGVLYFGDGKVRASYTPADGL
                     GEGPVAALQLDRDGALWAATQQGGLSRIKDGRVATLTTRNGLPCDTIHWSMEDDDRAL
                     WLYAACGLVRITRTELDAWIADPKRRIETAVWDAADGVRLNAVAASSYNPTVAKSSDG
                     RLWFLSKEGVQVVDPRHLPFNRVPPPVHIEKVFADGSLYWQPLPGETVSTVTLPPLTR
                     ELQIDYVGLSFVAPESMQFRIKLEGQDDDWRVPVNPRHSRYTNLRPGKYTLRVKASNN
                     SGVWNEQGDALELWVAPAYYQTNWFRALCVAAGIALLWAAHQVRLRQLHHQFDMTLDA
                     RVGERTRIARELHDTLLQSAHGVLLRFQTVSELLPERPTEAKATLDRAIEQTAAFITE
                     ARDEVQGLRDSTIQTNDLALAINSLGEGLANDFVARGRVTFHVAVEGESRDLHPVVRD
                     EIYKTATEALRNAFRHAQARSIEVEIRYDNEQFRLRVRDDGRGIDR"
BASE COUNT          504 a          956 c          929 g          529 t
ORIGIN      
        1 cgatcgatgc ccctgccgtc gtcccgcacc cgcaggcgga attgctcgtt gtcgtagcgg
       61 atttccactt cgatgcttcg ggcttgggcg tgccgaaatg cattccggag cgcttcggtg
      121 gccgtcttgt agatctcgtc acgaacgacg ggatgcagat cgcgtgactc gccttccacg
      181 gcgacgtgga atgtgacccg tccgcgagcg acgaagtcgt tcgcgagtcc ttctccgaga
      241 ctgttgatgg ccagcgcgag gtcgttggtc tggatggtgg aatcccgcaa tccctgcact
      301 tcgtcccgag cttcggtgat gaatgccgcc gtctgctcga tcgccctgtc cagcgtagcc
      361 ttcgcttctg tcgggcgctc cggcagcagt tcggaaacgg tctggaaccg cagcagcaca
      421 ccgtgcgcgc tctgaagcag cgtatcgtgg agctcccgcg caatgcgcgt gcgctctccg
      481 acgcgcgcgt ccagcgtcat gtcgaactga tgatggagtt gccggaggcg gacctggtga
      541 gccgcccaca gtagcgcgat gccggccgcc acgcacaatg cgcgaaacca gttcgtctgg
      601 tagtacgcgg gcgcgaccca gagctccaac gcgtcaccct gctcgttcca caccccgctg
      661 ttgttcgagg ccttgacgcg gagggtgtac ttccccggcc tcaggttcgt gtagcgcgag
      721 tggcgcgggt tgaccggcac tctccagtcg tcgtcctggc cctccagctt gatgcggaac
      781 tgcatgctct ccggcgcgac gaagctgaga ccgacatagt cgatctgcag ctcacgggtc
      841 agcgggggaa gagttacggt cgacaccgtc tcgcccggta gcggttgcca atagaggctc
      901 ccgtcggcga agactttctc gatatgtacc ggcggcggga cccggttgaa aggaagatga
      961 cggggatcca cgacctggac gccctctttc gagaggaacc acaacctccc atcgctggac
     1021 ttcgcgacgg taggattgta cgaggaagcc gcaaccgcgt tgagcctcac accatccgct
     1081 gcatcccaga cggcagtctc aatcctgcgc ttcgggtcgg cgatccacgc gtccagttcg
     1141 gttcgggtga tacgcacgag cccgcaggcc gcgtacaacc agagcgcgcg atcgtcgtct
     1201 tccatcgacc agtgaatcgt gtcgcagggc agaccgttcc tcgtcgtgag ggtggcgacg
     1261 cggccatctt tgatacggct gaggccgccc tgctgggtgg cggcccacag cgcaccatcg
     1321 cgatcgagtt gaagggcggc caccgggccc tcgcccagtc catccgcggg cgtgtacgag
     1381 gcgcggacct tgccatcgcc gaaatacagc acgccaccgc cgttccagaa tgccagccag
     1441 acaccgccct ggtccgggac cacggccttg gcctgttgat gacgccccat caccggccag
     1501 tcgagagtct cgaccaaacg cccatcccgc atgtgcagca ggctcttgtt ggttgaaagc
     1561 cagagaccac ccgccccgtc ccccttcatg gaaaacactt ctttgctggg gacgcccggt
     1621 acgcctatga agcgatcgtc gttcgtcaga taggccaagc cgccattcgt gaacacccag
     1681 agtcgaccac gatcatcctg gacgagagac tgcgcccggt catgcggcag tccactggcc
     1741 gtgtcgagga ccgtggcttg tccgttcttc caccgggtga ggccatcttc ggcggcaacc
     1801 cagatgctcc catccgtggc cgcgagcacg gacaaggccg cgtcgctgga gaggccttgt
     1861 ttcgcggaca gcgtagttac ggggagctct cgaaaccggt cgagtcctcc cgtggtggcg
     1921 acccagatat tgccttcacg atcctcgaag aggctgcagc tgatatcgcc cgaaagccca
     1981 tctgctttcg tgaatatgtc cgtcctgccg ttgtgtacgt ggacgagccc gcgttggcgg
     2041 gtgccgatcc agagtcctcc atcgcggtcc cgaaggagtt tgttggaatc gacattgcgg
     2101 tcggggagca ggcgatcgcg attggtcgca tcccgaatcg gatgggactc gagtctgtct
     2161 ccggcgatct gcaggagtcc tccgccgctc acgccgatca gcacgtgtcc gtcgtcggtt
     2221 cgagtgagat caccgatccg cattcccggc gcctcatagc gccggggaga gccgggtttc
     2281 caccgccaga ctccggactc agcaccggcc cagagagagc ctgcgctgtc ctcgaacaga
     2341 ctccacacga atgtgccgaa agccccgccc tgcagatcac attgcgcgtg gccgctccgg
     2401 attgcgcaca gctggcccgt cggagcatcc gatgtcgagc ccaataggcc ggcccagacg
     2461 gttccctccc ggtcttcgag cagcgatgtg acgaaccggt cgccgatctc cgggtaggac
     2521 gtcagcttgt cgccgttcca gctgacgagg ccggcaaacg tgccgatcca gagggtcccg
     2581 tcgcgcgtca cgagcagggc gtagggcctc tcggggagct tttgccccgc cggtgggtgc
     2641 caagggaccg cctgaacgcc atcgaagcgg aacaatccga attcgctgcc tagccagagg
     2701 tagccgtcgg gcgtttgcgc catcgcgaag atggcgcccg cggagaagcc gttccgggcg
     2761 gtccacgccg tgtgcccgtg ttggctgacg tccagcgagg ggtcgagcgc aagaagggga
     2821 gcagaccaga gcaatagcgt gagcgtgatg gtcttgatag cggccatctt cgcgagcacg
     2881 gcagcctcct acgggcgggg cgcgcggccg cggaacga
//
LOCUS       DASRJQ010000083         5723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1912584, whole genome shotgun sequence.
ACCESSION   DASRJQ010000083 DASRJQ010000000
VERSION     DASRJQ010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1912584"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..1105
                     /locus_tag="VFB89_01915"
     CDS             62..1105
                     /locus_tag="VFB89_01915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZM26089.1"
                     /translation="MRRGLLAGVLLLAACGHAGSEDEAGPADSTRVPVGLGSVVRDSI
                     VEQATVTGRLEARPGGAALLAAPAAGVVRAVVAQVGDRVRPRQVLLELEVPELVADAE
                     QRASAAAQAQREAERQQRLLADGITSARQAEEASAAARQAQTAAIAARNLLTRTRVSS
                     PIAGRVQQIYVQRGERVDAGKPLAQVIAGDTLDLVGAAPVATLGRLRQGQNAMIAQEG
                     DSTRYRGWVAALAPGVDSATGAGQVVLRVPNPDGALRAGAGAQGWIRLGVRHDALVVP
                     DSAIVLQGDSSTVFVVGRDSVAHARTVVRGAHQEGRTEVRGNLAPGDRIVTTGAFGLQ
                     DGMRVAPFGVTDR"
     gene            1102..4164
                     /locus_tag="VFB89_01920"
     CDS             1102..4164
                     /locus_tag="VFB89_01920"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZM26090.1"
                     /translation="MSLAGLALRHRAALYLCISLLTAGGVWALATLPAGIYPEVTYPR
                     IAVVARGGTFEAEEMTVAVTRPLEQAVSGIVDLRRIRARTVRGGSELSLDFSPRADMQ
                     FALSQVQGRLDAVRGSLPPGLDLTAERLTPSVFPMLQYELTGADPLVLRDLAEFVVRP
                     RLTGLPDAGHVEVQGGRVREISVQLDPVRLVANHVTADQVAQAITTQDQAETVGRVDR
                     DYRQYGIVVSGLTNTPEAVGHVVVKQAADRPLRVADLGSVGYGAEDLFQIVAGNGRPA
                     ALVNVARQPNGNTLRLQAEASAAIAAIRPLLPKSVRLEAVYDQAALVRDSIRSVRDAM
                     LLGGALAVLVLLAFLGRWRTTMAAAIVLPLTVAITVLGLALFGDSLNLMSLGGLAVAI
                     GLVIDDAVVVVENIERRLELHPEEPPSEVVREATDEIFGPVAGSTLTTVVVFLPLGLL
                     EGVVGEFFRSFALALTIAVLLSLALAMTLIPTLVGRRGAARSKRPSRRVSLERLGGRY
                     GLAVAWLLEHRATALGVAVFLAISGLALMRFVGTGFLPEMDEGGFILDYWAPAGTALS
                     ETNRQVGVLERILLRDPDIQAFTRRTGSELGFAATAPNTGDFTVLLKPRRRRSASVFE
                     VIDRVRAAAEAEAPAVRVEFVQLLQDVIGDLAGSPEPVELKLFSSDHAAAERAAVSVA
                     KAIEPTPGLVDLFNGVQGPSPELRLQLDPVRLARLGLTATEVEAQARSALFGANAGTA
                     REPDRLIPIRVRLPDSVRFSRDLTAQVPVVGPSGWTQLGALGSVFDTAGASELRRENL
                     RPYVPVTGRTSGRSLGAVMADVRAAAARVPLPAGVTMEVGGQYASQRTAFRQLLGIFA
                     LGTGAVLLVLVAQFDGFRGPLSIVLVAPLGLVGTLLLLLAAGVPFNVSSFMGIILLVG
                     LVVKNGILLLDAARRTRAAGVAYHGALVEAGRLRLRPILMTTLCTLAGLAPLAFGFGA
                     GAELQRPLALAVVGGLTISTGVTLLLLPVVLDAIGALGPRGTAAESAA"
     gene            4239..4823
                     /locus_tag="VFB89_01925"
     CDS             4239..4823
                     /locus_tag="VFB89_01925"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HZM26091.1"
                     /translation="MNLRWSTLLALLVLALPAGLLAQGEAGIKGGVSFGDISNKGLLP
                     GNLDKRTGFAAGVYIGYRTGILGFDIEGLYAQRGAESDSVTATAPSKLDYIDVPVYAK
                     LLIPIELIQPYIYAGPQVSFEVRCRTANGQDCPSGHEKHDYGMVFGAGIGFGGLGLEG
                     RYVYGLKDLHLSTITSAESYETRSFLILLRIGRT"
     gene            5007..5642
                     /locus_tag="VFB89_01930"
     CDS             5007..5642
                     /locus_tag="VFB89_01930"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZM26092.1"
                     /translation="MAIRLGDDAPDFLAQTTEGPIQFHQWKAGQWAVLFSHPKDFTPV
                     CTTELGSLSALRPEFDQRGVKVIGLSVDPLESHRRWTDDIHDVTGHALNFPLIADPDR
                     TVSRLYDMIHPTASETVTVRSVFVIGPDNKVKLTLTYPASTGRNFQELLRVVDSLQLT
                     ANHGVATPADWEDGDDVIVVPALSDDEARACFPRGFVAKKPYLRVTPQPNR"
BASE COUNT          880 a         1837 c         1921 g         1085 t
ORIGIN      
        1 acgagcttgc ctaccaggaa gccctggccg actgggatgc gctgctcggg aaagtcgagt
       61 gatgcggcgc gggttgttgg cgggcgtgct cctgctggcg gcgtgcggcc acgccggctc
      121 ggaagacgaa gccggccccg ccgattctac ccgggtcccc gtcgggctgg gcagcgtagt
      181 ccgtgattcg atcgtcgagc aggcgacggt gacaggtcgc ctggaggcgc gccccggcgg
      241 cgccgctctg ctggcggcac cggccgcggg agtcgtgcgc gccgtggtgg cccaggtcgg
      301 cgatcgggtt cggccacgcc aggtacttct cgagctcgag gtacccgagt tggtcgctga
      361 cgccgaacag cgggcttcgg cagcggcaca ggcccagcgg gaagccgaga gacagcaacg
      421 gctgctggca gacgggatca cctccgcccg acaagccgag gaagcaagcg ccgcggcccg
      481 ccaagctcag acggcggcaa tcgcagcgag gaatctgctc actcgtactc gcgtctccag
      541 tccgattgcc ggccgggttc agcagatcta tgtgcagcgg ggtgagcgag tggatgccgg
      601 gaagccactt gcgcaggtca ttgccggaga cacgctcgat ctggtagggg cggctccggt
      661 ggcgaccctt ggtcggctgc gccaaggcca aaacgccatg attgcgcagg agggcgactc
      721 gacgcgatac cggggctggg ttgcggctct cgcgcccggc gtcgactcgg ccaccggtgc
      781 gggccaggtc gtgctccgcg tgccgaatcc cgacggcgcg ttgcgagcgg gcgccggcgc
      841 ccagggatgg atccgcctcg gcgttcgcca tgacgcgctc gtcgttcccg actcggccat
      901 cgttctccag ggtgacagca gcacggtctt cgtggtgggc cgggactcgg tggctcacgc
      961 gcgtaccgtt gtccgcggcg cgcatcagga agggcgtact gaagtccgag gaaacctggc
     1021 gccgggggat cgaatcgtca ccaccggcgc attcggactg caggacggaa tgcgggtcgc
     1081 tccattcgga gtcacagacc ggtgagcctc gccggcctcg cgctccgcca tcgtgccgcg
     1141 ctctatctct gcatcagcct gctgactgcg ggtggcgtct gggcgttagc gactctgccc
     1201 gccggcatct accctgaggt tacctaccca cgcatcgcgg tggtcgcccg aggcggcacc
     1261 ttcgaagccg aggaaatgac cgtcgcggtt acccgtccgc tggagcaggc cgtgagcggg
     1321 attgtggacc tgcgccgcat ccgggcccgc accgtgcgtg gaggctccga gctgagcttg
     1381 gacttcagtc cgcgcgccga catgcagttt gcgctcagcc aggtccaggg ccggctcgac
     1441 gccgtccgcg gctcgctacc ccccgggctc gatttgaccg ccgagcgact tacgccctcg
     1501 gtgttcccga tgctgcagta cgagctgacg ggcgccgatc cgctcgttct gcgggacctg
     1561 gcggaattcg tcgtgcgacc tcgtctgacc ggccttcctg atgcgggcca cgtcgaggtg
     1621 cagggcgggc gggtgcgaga gatcagcgtc cagctggatc cggttcgtct cgttgctaac
     1681 cacgtcactg ctgaccaggt cgcgcaggcg atcacgacgc aggatcaggc tgagacggtt
     1741 gggcgggtcg atcgcgacta tcgacagtac gggatcgtcg tctccggtct caccaatacc
     1801 ccggaggcag tagggcatgt ggtagtgaag caggcggccg accgtcccct ccgcgtagcc
     1861 gatctgggca gcgttggcta cggggccgag gatttgttcc agatcgtcgc aggaaacggt
     1921 cggcctgcgg cactggtaaa cgtcgcgcgc caacccaacg gcaacacgct ccggctccag
     1981 gctgaagcca gcgcggcgat cgcggctatc cggccgctgc tgcccaagag tgtgcggctc
     2041 gaggctgtgt atgaccaggc agcgctggtg cgcgattcga tcaggagcgt gcgagatgca
     2101 atgctcctcg gtggtgcgct cgcggtcctg gtgttactcg ccttcctggg caggtggcgc
     2161 acgacaatgg ctgcggccat cgtcctgccg ctcacggtag ccattaccgt tcttggcctg
     2221 gcactgttcg gcgattcgct gaatctcatg agcctgggcg gtctggctgt agcgatcggg
     2281 ctcgtgatcg acgacgcggt ggtcgtggtc gaaaatatcg agcggcggct cgagcttcat
     2341 ccggaggagc caccgtcgga ggtcgtccga gaggcgaccg atgaaatctt cggcccggta
     2401 gcaggctcga ccctcaccac cgttgtcgtg tttctgcccc ttggcctgct cgaaggcgta
     2461 gttggcgagt tcttccggtc attcgccctc gcgctgacga tcgccgtcct gctctcgctc
     2521 gcacttgcca tgactctcat tccgacgctg gttggtcgtc gtggtgcggc aaggtcgaaa
     2581 aggccgtctc gtcgtgtgtc gctcgagcga ttgggcgggc gttacgggtt ggcggtcgcg
     2641 tggttgctgg aacatcgtgc tactgcgctg ggtgtggctg tcttcctcgc gatctcggga
     2701 ttggcgctca tgcgcttcgt cggcaccggt tttctgcccg agatggacga aggcggattc
     2761 attctggatt actgggctcc tgcaggaacg gccctgagcg agaccaatcg ccaggttggc
     2821 gtcttggagc gaatcctgct tcgcgacccc gatatccagg cgttcacccg gcgcaccggg
     2881 agcgagcttg gcttcgccgc gactgccccg aataccggtg acttcacggt gctgctcaag
     2941 cccaggcggc gccgctccgc ctcggtgttc gaggtcattg atcgcgtccg ggcagcggca
     3001 gaggccgagg cgccggcggt gcgggtagag ttcgtccagt tgctgcagga cgtgattggc
     3061 gacctcgcag gttcacccga gccggtggag ctcaagctat tcagcagcga ccatgctgcc
     3121 gccgagcgcg ccgcggtgtc ggtggcaaag gcgatcgagc cgacgcctgg cctggtagac
     3181 ctgttcaacg gtgtgcaagg tcccagtccg gagctacgcc tgcaactcga tccggttcgt
     3241 ctggcgcgac tgggtcttac cgcaacggaa gtcgaagctc aggcccgctc tgcgctgttc
     3301 ggtgccaatg ccgggacggc gcgggaaccc gaccggctga ttccgattcg agtgcgactt
     3361 cctgacagcg tgcgcttcag tcgcgacctc acagcgcagg tccccgtggt cggaccctcc
     3421 ggctggacgc agctcggcgc gctcggctca gtcttcgata cggccggcgc cagcgaactg
     3481 agacgcgaga atctgcggcc gtacgtgccg gtcaccggta ggaccagtgg tcgcagtctc
     3541 ggcgcggtca tggccgatgt ccgtgctgcg gctgcgcgcg tgccgctccc agccggcgtg
     3601 accatggagg tgggagggca atacgccagc cagcgcactg cgtttcgtca gctgctcggc
     3661 atattcgctc tgggcacagg agccgtcctg ttggtcctcg tagctcagtt cgacgggttc
     3721 agaggtcctc tttctattgt gctggtggcc ccgctcggac tggtgggaac gctgctcctg
     3781 ctgcttgctg ccggagttcc gttcaatgtg tcgagcttca tggggatcat tctgctggtg
     3841 ggacttgtcg tgaagaacgg aatcctcctg ttggatgctg cgcgccgtac tcgagcagcg
     3901 ggagttgcct atcatggcgc gctagtggag gcggggcggc tgcgtctccg tccgattttg
     3961 atgactaccc tttgtacgct ggcgggcctg gcgcccctgg cgttcggctt tggagccgga
     4021 gctgagcttc aacggccgtt ggcgctggcg gttgtcggcg gccttactat ctcgacaggg
     4081 gtgacccttc tgctgctgcc cgtagtgctc gatgccatcg gcgccctcgg cccaaggggc
     4141 acagccgccg agtccgccgc ttgaactcct gtgaggctcg tcacgtcgcc aaaatcctgg
     4201 cggcctttac gctactctga ccccatccag gacatcgtat gaacctccgc tggtctacgc
     4261 tcctcgcgtt gctcgtgctc gcgcttccgg ccggtttgct cgctcaggga gaggccggca
     4321 tcaagggcgg tgtgtcgttc ggggacatct ccaacaaggg cttgctgccc ggcaatctgg
     4381 acaagaggac cggctttgcg gccggcgtgt acattggcta tcgaaccggg atactcggct
     4441 tcgacatcga aggtctttac gcgcagcgcg gtgccgagtc cgattctgtg accgctaccg
     4501 cgcccagcaa gctcgactat atcgacgtgc cggtctacgc caagctcctc ataccgatcg
     4561 agctcattca gccgtatatc tatgccgggc cgcaggtatc gttcgaggtt cgctgccgta
     4621 ccgctaacgg ccaggactgt ccgtccgggc atgagaagca cgactacgga atggtcttcg
     4681 gagcagggat agggttcggt ggactgggtc tggaaggccg ctacgtctat ggtctcaagg
     4741 atctccatct gtccaccatc accagtgccg agagctacga gacccgatcc ttcctgattc
     4801 tgctgcgcat agggcgtact tgacgagcgg cgcctgaagt cgcataatca ttcagcgcgg
     4861 cgatttcccc gacttgatgg gccgcgcggc cggacgcttc cggcttcacc atctaagtcg
     4921 cggcaagcgg cccgcgacag tcttcgcggg ccgcttagcg ttcccggacg gggaaccgcc
     4981 gcgccgcaca cgttaaggag gcagcaatgg caatccgcct gggagacgac gcgccagact
     5041 tcctggccca gaccaccgaa gggccgatcc agtttcacca gtggaaggca ggccagtggg
     5101 ccgtgctctt ttcccacccc aaggatttta cgcccgtctg caccaccgag ctgggaagcc
     5161 tttccgccct gcgacccgaa ttcgaccagc gaggcgtcaa agtcattggc ttgagcgtcg
     5221 acccgctcga gtcgcaccga cgctggaccg acgacatcca tgacgttacc gggcacgcgc
     5281 tcaactttcc gttgatcgcc gatccggacc ggaccgtttc ccggctgtat gacatgatcc
     5341 acccgactgc gagcgaaacg gtgactgtgc gctccgtctt tgtgatcgga ccggacaaca
     5401 aggtgaagtt gaccctgacc tatccggcca gcaccgggcg gaacttccag gaactgctcc
     5461 gagtcgtcga ctcgctccag ctgaccgcca atcacggtgt cgccaccccg gcggactggg
     5521 aggatggcga cgatgtgatc gtggttccgg cactctcgga tgacgaggcg cgcgcctgct
     5581 ttcccagggg cttcgtggca aagaagcctt accttcgcgt gacaccgcag cccaaccgat
     5641 gagcgagtca gtgggcctca ccatgcgggc tgtcgtccgc cgccaaaaca gtgcggtcca
     5701 cttcgtggcc gagaccggca atg
//
LOCUS       DASRJQ010000084         6719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2755368, whole genome shotgun sequence.
ACCESSION   DASRJQ010000084 DASRJQ010000000
VERSION     DASRJQ010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6719
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2755368"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1749)
                     /gene="mrdA"
                     /locus_tag="VFB89_01935"
     CDS             complement(<1..1749)
                     /gene="mrdA"
                     /locus_tag="VFB89_01935"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZM26093.1"
                     /translation="MTGSDSYRLGERARVARWIIVGAFLVLLGAFFRTQIIQHEKFQL
                     KAETNRLRPIPLTAPRGTIYDRHGEIIAENVPGYSVKLLAPSADSLKAVLARVREVVP
                     LDSSAVAEVMRRFQTARYQPVVVFGDATFEIVSKLEEHRVALPGLVIQAEPKRLYPAR
                     NAVAHLVGYVGEVTDADLESDRYEGLEPGAIVGKAGLEQQYDDSLRGTAGVRYIEVNA
                     RGRLVREEASSASLSPTPGRPIETTIDLDLQRFIDSTWPPGVRGAMVAMTPSGEVRAL
                     YSAPSFDPNAFIGGIAPQLWRSLNTDEARPLLNRALQARYPPASPFKLAIAAMALKRG
                     LIGLDSHMPVPCRGGLRMGNRVFRCWKKEGHGSLDLIGAIAASCDVYFYQVGLRLGLD
                     AIIEDGVLMGFKDKAGIDLRNEINPIYPSSTAYFDRLYGARRWSPAATTLNFSIGQGE
                     NTQTLINMVHFYAGLAGSGEEVSPFLVRPRDDAKARPLGLGTDLLSGLRHALIAVVER
                     GTAAASRHEDLQVAGKTGTAQNPHGKDHGWFIGFAPADKPELVVGGIMEFAEHGTVVA
                     PYVVAALRRYILGPDTS"
     gene            complement(1746..2243)
                     /gene="mreD"
                     /locus_tag="VFB89_01940"
     CDS             complement(1746..2243)
                     /gene="mreD"
                     /locus_tag="VFB89_01940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HZM26094.1"
                     /translation="MNGSRTARVHLAIIFGTLILLHFYVRPRLFDTRLAPDFLLFGLV
                     VFAMRSGPGAGALAGFLVGLATDALSPAKFGAATLADTIVGYAAAWGRAVFFADNVLV
                     NAGFVAVGLWLRDLILLMASGTPQGQLLVELGVYSPLQAIITAAAGGLVLLAFRQWFA
                     VRLDG"
     gene            complement(2240..3337)
                     /gene="mreC"
                     /locus_tag="VFB89_01945"
     CDS             complement(2240..3337)
                     /gene="mreC"
                     /locus_tag="VFB89_01945"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HZM26095.1"
                     /translation="MFVGCLILAIAALFSPERITGGFANGLRQTVLAPLVWLQEEAEL
                     GRGSRARLRELLAERDSAAYAAQVLPSLTAENERLRNLLGLSHRLRTPYVAAEVLHQA
                     QVTDARMLLLNVGTAQGVAGFDPIVAPDGLIGVIWNASRTSSVAMTWAHPEFRVSAFT
                     ANGSAFGIVAASTSGVGSDAYLEFRGVPYRDSVVPGTVVLSSGLGGVYPKGIPIGTVV
                     GVIREEAGWARVYRVRPAANPGSTAHVLVLTGRRVGSIADAYPVDTLEPAARPDSAPL
                     PKLAPTLGGGNEPKAPARARVDSAGKVPARAKPDSMGKPPARAKPDSAVKPKPKPKPK
                     LPPADSARPAPPPAAPAPTPAPKPALGDSNP"
     gene            complement(3385..4422)
                     /locus_tag="VFB89_01950"
     CDS             complement(3385..4422)
                     /locus_tag="VFB89_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HZM26096.1"
                     /translation="MKLPSLNLASFLPANEIAVDLGTANTLVYVKGEGIVLNEPSVVA
                     IERASGKIKGIGLEAKRMLGRTPDGILAVRPLKDGVIADFDVTEKMLRYFLKTIIDKH
                     VFRVKPKVIVCVPSGITEVERRAVRDSAQTAGAKQVMMVAEPMAAAIGVGLPVETPTG
                     NMVIDIGGGTTEIAVIALSGIVSDTSIRTGGDELDQAIVQFMRKNYNLLVGEPTAEAI
                     KIKIGSAAPVGEEREMEVKGRDLVSGIPKTVRVHSSEIREAVQEPIQQIVDAVRRALE
                     ITPPELASDIVDRGIVMTGGGALIRGLDILLAQETGLPVHVDEDPLTCVVRGTGRILD
                     DFEKYRSVLSF"
     gene            complement(4491..5030)
                     /gene="dut"
                     /locus_tag="VFB89_01955"
     CDS             complement(4491..5030)
                     /gene="dut"
                     /locus_tag="VFB89_01955"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HZM26097.1"
                     /translation="MSPPFSEESERPPKSGSIRVQIARLPHGEGLPLPARATAGSAGY
                     DLSSAEDGSLAPFERRMFCTGFSIAVPDGYECQIRPRSGLALRHGITLPNTPATIDSD
                     YRGELMVALVNLGTEPFVVTRGMRIAQMVIARVERAEFTEVAELPATQRGAGGFGSTG
                     VGREQAETDGTGHERTGAA"
     gene            complement(5011..6123)
                     /gene="ald"
                     /locus_tag="VFB89_01960"
     CDS             complement(5011..6123)
                     /gene="ald"
                     /locus_tag="VFB89_01960"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HZM26098.1"
                     /translation="MQIGVPKEIKTNENRVALVPGGAEALVAQGHTVFLERGAGEGSG
                     FDDAAYEAAGARVLPTADEVWSRADLIMKVKEPIEVEWPRMRPGQVMYTYFHFAADER
                     LTRAVIASQAIAVAYETVQLPSGELPLLTPMSEVAGRMAVQAGAKYLEKVYGGRGILL
                     GGVPGVAPAEVVILGGGTVGINAAKMAAGLGAHVTILDISLDRLRYLDDVLPANCDTW
                     YSNRHNILDAASRADLVVGAVLLPGARAPHLLRREDLKRMKQGAVIVDVAVDQGGCVE
                     TIKPTTHENPTYFVDGILHYGVANMPGGVPRTSTLALTNATLQGALRLAKHGWQEACR
                     RDEGLRKGLNVVAGKVVYPGVAEAFKLPLVDVAAVL"
     gene            complement(6135..>6719)
                     /locus_tag="VFB89_01965"
     CDS             complement(6135..>6719)
                     /locus_tag="VFB89_01965"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HZM26099.1"
                     /translation="RPPVQPEPAVRGVSRAETRDSSQTHIVFGTDTFPVRDPRRFALG
                     ILVNSSGGGMSSRLFQRVREELGLAYAIFAFKQFFQSTGQLGVYVGTQAATADQAIEA
                     IRGEYNRLAKQGLPAEELKDGKRQLKGQVVLSLESPSARMHRLAGFVLHNDHYRTLSD
                     MLAEIDAVTSEQTAAIAAEFFPADRQTMVRLGPE"
BASE COUNT         1191 a         2244 c         2149 g         1135 t
ORIGIN      
        1 ggaggtatca gggccaagga tgtatcgtcg cagcgccgcg acgacgtacg gcgccacaac
       61 cgtgccgtgc tccgcaaact ccatgatgcc gcccacgacc agctcgggtt tatcggccgg
      121 ggcgaatcca atgaaccacc catggtcctt gccgtgagga ttctgcgccg ttcccgtctt
      181 gccggcgacc tgcaggtcct cgtggcggga cgcggccgcc gtgcctcgct ccacgaccgc
      241 gatcagcgca tggcgaagcc cgctcagcag gtccgttccc aagccgaggg gccgcgcctt
      301 ggcgtcgtct cgggggcgaa ccaggaacgg gctcacctct tcaccgcttc ccgcaagtcc
      361 ggcgtagaag tggaccatgt tgatcaaggt ctgggtgttt tcgccctggc cgatcgagaa
      421 gttgagcgtc gtggcagccg ggctccaccg ccgggcaccg tagaggcggt cgaagtacgc
      481 tgtggatgag gggtatatgg ggttgatctc gttgcgcaga tcgatccccg ccttgtcttt
      541 gaaccccatc agcacgccgt cttcgatgat ggcgtcgagg cccagacgaa ggccgacctg
      601 atagaagtag acgtcgcagc tcgcggcgat ggccccgatc aggtcgagtg acccgtggcc
      661 ttcctttttc cagcagcgga agacccgatt gcccatgcgg agcccgccgc gacagggaac
      721 gggcatgtgg ctgtcgagcc cgatcaggcc ccgcttgagc gccatagcgg cgatagccag
      781 cttgaacggt gacgccggcg ggtagcgcgc ctgaagcgcc cgattgagca gcggacgggc
      841 ttcgtcagtg ttgagcgatc gccagagctg cggggcgatc ccgcctatga acgcattggg
      901 gtcaaaagat ggcgcggagt aaagggcgcg cacctcaccg gatggtgtca ttgcgaccat
      961 tgcgcctcga actcccggag gccaggtgct gtcgataaag cgctggaggt cgagatcgat
     1021 ggtggtttct attggccggc cgggtgtcgg agagagtgaa gcagacgacg cttcttcgcg
     1081 aaccagccgc ccgcgggcat tcacctcgat gtagcgcaca cccgctgtgc cccgcaggct
     1141 gtcgtcgtac tgctgctcga ggcccgcctt gcccacgatc gcgcccggct caagcccctc
     1201 gtagcgatca ctttccaagt cggcgtcggt tacctccccc acatagccga ccagatgcgc
     1261 cacggcattg cgtgcggggt agagccgctt gggctcggcc tggattacca gaccgggtag
     1321 cgcgacccga tgctcctcca gcttggagac gatctcgaag gtcgcatcac cgaagacaac
     1381 cacgggttga taccgtgccg tctggaagcg acgcatcacc tcggcgacgg cactgctgtc
     1441 gagtggaact acttcccgca cccgggccag aactgctttc agcgagtcgg cggagggggc
     1501 caacagcttg accgagtaac ccggcacgtt ttcggcgatg atctccccgt gccggtcgta
     1561 gatagtcccg cgtggcgctg tgaggggaat gggacggagc cggttggtct ccgccttgag
     1621 ctggaacttc tcgtgctgga ttatctgcgt acggaagaac gcgcccagca gcacgagaaa
     1681 cgcgcccacg atgatccagc gggccacccg ggcccgctca cccagccgat acgaatcgga
     1741 tccggtcatc catccagcct cacggcaaac cactgccgga aggcgagcag caccagcccg
     1801 ccggcggctg ccgtgatgat cgcctggagc gggctgtaga cgcccagctc gaccaggagc
     1861 tggccctgag gcgtgccgct ggccatcaga aggatgaggt cacgcaacca gaggcccacg
     1921 gcgacgaaac cggcattgac cagcacattg tcggcgaaaa agaccgcccg cccccacgcc
     1981 gctgcataac cgacgatcgt gtccgccaga gttgcagccc cgaatttggc cggcgagagc
     2041 gcatcggtgg caaggccgac cagaaagccc gccagggctc ccgcgccagg cccgcttcgc
     2101 atcgcgaaca cgaccagacc gaagagcagg aaatccggcg ccagccgggt gtcgaacaac
     2161 cgcggccgca cgtaaaaatg aagcaggatg agcgtcccga agatgatggc gagatgcacc
     2221 cgcgccgtgc gtgagccgtt cacgggttcg agtcccccag cgccggtttc ggagcgggag
     2281 tcggagccgg ggccgccggt gggggcgcgg gccgggccga gtcagccgga ggcagcttgg
     2341 gcttgggctt gggcttcggc ttgaccgcgg aatccggctt ggcgcgcgcg ggcggcttgc
     2401 ccatggaatc cggcttggca cgcgcaggca ccttgcccgc ggagtcgacc cttgcgcgtg
     2461 caggtgcctt gggctcattg ccgccgccta gcgtcggcgc cagtttcggc agcggggcgg
     2521 aatccggtcg agcggccggc tcgagcgtat ccaccggata ggcgtcggcg atagacccca
     2581 cacgtcgacc agtgagcacc agaacatgtg cggtcgagcc cgggttcgcc gccggcctca
     2641 cacggtagac ccgggcccag ccggcttcct cccgaattac gcctaccacg gtcccgatcg
     2701 ggatcccctt gggatagacg ccgcctagac ctgaggagag gaccaccgtt cccggaacca
     2761 ccgaatcccg gtacgggacg ccgcgaaact cgaggtacgc atcgctgccc acgccgctgg
     2821 tcgacgccgc aacgatgccg aaggcgctgc cattcgcggt aaaggcactg acccggaatt
     2881 cgggatgtgc ccaggtcatc gcgacgctgc tggtgcggct cgcgttccag atgacaccga
     2941 tcaggccatc cggcgcgacg atgggatcga agccggctac accctgcgcg gttcccacgt
     3001 tcagcaacag catgcgagcg tcggtgacct gcgcctggtg cagcacctcg gccgcgacgt
     3061 agggcgtccg gagacggtgg ctcagcccca gcagattacg cagtcgctca ttctcggcgg
     3121 tcaatgacgg aagcacctgc gctgcgtagg cggccgagtc gcgctcggcg aggagctccc
     3181 ggagccgggc tcggctcccg cgacccagct ccgcctcttc ctgaagccac accagcggtg
     3241 cgaggacggt ctgccgcagg ccgttcgcaa agcctccggt gattcgctcg ggcgagaaca
     3301 acgctgctat cgcgaggatc aggcagccga cgaacatcgc ggtgtcccgc cgactgaaac
     3361 ctcggtccga cgcggggaac atccctagaa cgaaagcacg ctgcggtatt tctcgaaatc
     3421 gtcgaggatg cgaccggtgc cacgcaccac gcaggtgagc ggatcctcat ccacgtgcac
     3481 cggcagtccg gtttcctggg cgagcagaat gtcgagtcct ctgatcaagg cgcctccacc
     3541 ggtcatcacg attccacgat cgacgatgtc gctggccagc tcgggaggag tgatctccag
     3601 cgcacgccgc acggcatcga cgatctgctg aatgggctcc tgaacggctt cgcgaatctc
     3661 ggaggaatgc acccgcacgg tcttgggaat gccggagacc aggtcgcgtc cttttacttc
     3721 catctcgcgc tcttcgccca ccggggcggc ggagccgatc ttgatcttga tcgcttcggc
     3781 ggtcggctcg ccgaccagca ggttgtagtt cttgcgcatg aactggacga tggcctggtc
     3841 cagctcgtct ccgccggtac gaatggacgt atccgacacg atgccggaca gcgcgatgac
     3901 cgcgatctcg gtggtgccgc caccgatatc gatcaccatg ttcccggtcg gggtctccac
     3961 cggcagaccc acgccgattg ccgccgccat gggctctgcc accatcatca cctgcttcgc
     4021 ccccgcagtt tgcgcggaat cgcgcaccgc gcgccgctcc acctcggtga tccccgaggg
     4081 aacgcacacg atcaccttag gcttcacgcg aaaaacgtgc ttgtcgatga tggtcttgag
     4141 gaagtaccgg agcatcttct cggtcacgtc gaagtcggcg atgaccccgt ccttgagcgg
     4201 acggaccgcg aggatcccgt cgggcgttcg ccccagcatc cgcttggctt cgagaccgat
     4261 gcccttgatc ttgccgctgg cgcgttcaat ggccaccacc gacggctcgt tcaggacgat
     4321 gccttcgccc ttgacgtaga ccagcgtatt ggccgtgccg agatcgaccg cgatctcgtt
     4381 tgcgggaaga aaggacgcca ggttcaaaga cgggagcttc atcgaatggc cgtcaggcgc
     4441 atggcgcgaa aagtaagaga gacacaggtt tgtcgggagt gggagggacc tcaggccgct
     4501 cctgtccgct cgtggccggt cccgtcggtt tccgcctgct cccttcccac accagtactc
     4561 ccgaatccgc cagcgccgcg ttgcgttgcc ggcagctctg caacctcggt gaactccgca
     4621 cgctcgaccc gcgcaatgac catctgcgcg atccgcatcc ctcgggtgac cacgaacggt
     4681 tcggttccca ggtttaccag cgccaccatc agctcccccc ggtagtcgct gtcgatggtg
     4741 gccggcgtat tgggaagcgt gattccgtga cgcagcgcga gtcccgatcg cggacggatc
     4801 tggcactcat atccatcggg aacagcgatg ctgaagccgg tgcagaacat ccgccgctcg
     4861 aatggtgcga gcgagccgtc ctcggcgctc gaaagatcat atccggcaga cccggcagta
     4921 gctcgggcag gaagcggcag cccttccccg tgcgggagcc gcgcgatctg aactcgaatc
     4981 gacccggatt tcgggggccg ttccgactcc tcagagaacg gcggcgacat cgaccaaggg
     5041 cagcttgaat gcctccgcga ctccggggta cacgaccttg ccggccacga cgttgagtcc
     5101 cttgcgcagt ccctcatcgc gccggcaggc ctcctgccag ccgtgcttgg cgaggcgaag
     5161 cgcgccctgg agcgttgcgt tggtgagtgc cagcgtcgac gtacggggaa caccacccgg
     5221 catgttggca acgccataat ggagaatgcc gtccacgaaa tacgtcggat tctcgtgcgt
     5281 tgtgggcttg atggtttcga cgcacccgcc ctggtctacg gcaacgtcga cgatgacggc
     5341 accctgcttc atgcgcttga gatcttcccg gcggaggaga tgcggggccc gcgcgccggg
     5401 aaggagcacc gcgcccacga ccaggtctgc ccgggacgcc gcgtccagaa tgttgtggcg
     5461 attggaatac caggtatcgc agttggcggg tagaacatcg tccaggtagc gcaggcgatc
     5521 gagcgaaatg tcgaggatgg tgacgtgagc gcccaggccc gccgccatct tagcggcgtt
     5581 gatgcccaca gtaccaccgc ccagaatgac gacctcggcc ggcgctacac ccggcacccc
     5641 gccgagcagg atgccgcggc ccccgtaaac cttctcgaga tacttggcgc cggcctgcac
     5701 cgccatccgg cccgcaacct cggacatggg cgtgagcagc ggcagctcgc cgctggggag
     5761 ctggaccgtc tcgtaggcta ccgcgatggc ctgtgaggcg atgacggcac gggtgagccg
     5821 ttcgtccgca gcgaagtgga agtacgtgta catcacttgc cccgggcgca tccgcggcca
     5881 ctcgacctcg atcggctcct tcaccttcat gatcaagtcg gcccgggacc agacctcgtc
     5941 ggctgtcggc agcacgcgag cgcccgcggc ctcgtaggcg gcgtcgtcga acccgctgcc
     6001 ttctcctgct cctcgctcca gaaaaacggt gtggccctgc gcaacgagcg cctcggcacc
     6061 gcccggcacc aaagcaaccc gattctcatt ggtctttatc tctttcggga cgccgatttg
     6121 catgggccct cttttcactc cggtcccaat cgcaccatcg tctgacggtc cgcagggaag
     6181 aattccgccg cgatggctgc ggtctgctcc gaagtaaccg cgtcgatctc cgctagcatg
     6241 tcgctcaagg tgcgatagtg gtcgttatgc agcacgaatc cggcgagccg atgcatccgg
     6301 gcgctgggac tctcgagcga cagcaccacc tggcccttca gctgccgctt gccgtctttg
     6361 agctcttcgg ccggcagtcc ctgcttagcc agccgattgt actctccgcg aatggcctcg
     6421 atggcctggt ccgccgttgc cgcctgggtc ccgacgtaga cgccaagttg accggtgctt
     6481 tgaaagaatt gcttgaatgc aaagatcgca taggcgagtc ccaattcctc cctgacgcgc
     6541 tggaacagcc gactcgacat gcccccacca gatgaattga ccaagatccc cagcgcaaag
     6601 cggcggggat cgcgcaccgg gaacgtgtcg gtgccgaaga cgatatgggt ttgggacgag
     6661 tcgcgcgttt cggcgcgact cacgccccga actgcgggct ccggctgcac cggtggccg
//
LOCUS       DASRJQ010000085         5291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_510181, whole genome shotgun sequence.
ACCESSION   DASRJQ010000085 DASRJQ010000000
VERSION     DASRJQ010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510181"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..139
                     /locus_tag="VFB89_01970"
     tRNA            47..139
                     /locus_tag="VFB89_01970"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:81..83,aa:Ser,seq:gct)
     gene            complement(189..626)
                     /locus_tag="VFB89_01975"
                     /pseudo
     CDS             complement(189..626)
                     /locus_tag="VFB89_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087945.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1227..4277
                     /locus_tag="VFB89_01980"
     CDS             1227..4277
                     /locus_tag="VFB89_01980"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZM26100.1"
                     /translation="MPAASPGALDREADRRQVTILFADLSGFTTLSERLDPEEVRALQ
                     TALFETLAQAITRHGGSVVKFIGDAVLAVFGAPVAHEDDPERALDTALEMLARGATLS
                     DAWTARLGQPVMLHIAVHTGPVVAGSLAAAAGAAGAVYDVTGDTVNTTSRLLGAAGPG
                     TILVSDATHALARHRFAFEPAGELTLRGKAEPIAVHHLLGALAEPGSARRLSHGLAAP
                     MVGRLDELEQLLAAFDRMQWGRAQVVTLIGEAGTGKSRLIAEMLGRVEADGRLAGTIV
                     RRAACSSLGEPTYGIFGALFREAYQVEANDSLDIARQKLAAGLQSLGARPEVVETIAP
                     VLSYMLGVEETLPRDGEPEQLKRQIMLAGRTLVEPHLQRGPLLIIVEDLHWGDVASVD
                     LLRDVVDHLSDRRLMMLFTHRSDMRPPVVARAAQSIIRLSPLSIDETRTLVDGLLGSV
                     AGDAIARVQDFVATRAGGNPLFVEEIVRSLMGKGVLVRQGDHWTCTAACQAIEIPATL
                     HGLLLSRIDRLPADARRLLQEAAVLGAVFDLALLRSIAVGSEAAEAAFARLAEADLIQ
                     QIGHDCSHYRFTHALVHEAVYQNLLLARRTDLHERTGRALERAAGPHPERLSDLEALG
                     HHWSLSPDKPRGARYLMAAGDWARSVYANDDAIRHYERALRTLADCQTCDDQVQAVRE
                     RLADLLTLTGRRAEALTHYEAVWQVVESAADRAGAARLHRKIGGLHWEAGDRERASAC
                     FAAGLDRLGDDGNPIERAQLFQEMGRLAFRAGDNAAAIAWAERALAVAASGEDASDPE
                     RAREAAATRAHAYNTLGVALARIGRLAEAVDQIEQSIGLAEVHNLLQASCRGYANLGV
                     LYSSLDPQRSIETCLRGLDTAKKVGDLGFQSRLYANLAVAYCALTDRCEAEGIEAAQA
                     AVDLDRRLGLLDHLAVPLIVLGQIYQCHGDHALAFASYEEALGLAEQVGEPQLLFPCY
                     DGLATLHLDAGNQVMAEAYLAKSQAVCERAGIEPDALMVLPFLS"
     gene            4306..4953
                     /locus_tag="VFB89_01985"
     CDS             4306..4953
                     /locus_tag="VFB89_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HZM26101.1"
                     /translation="MTHAPPPVSPGEPAPDFTLSTVDGEELVSLANYRGSPLFLALLL
                     GLWCPFCRRQIAQLGTFEPKLKAVGVESLGVVATQPDNARLYFRFRPTRLRLAADPDL
                     STHRAYGVPKPVPTPEFMHALETIRINPDGAFPVPLPIVEAAATVAELDGYVKNETDR
                     ADMQRQWPQLKAHYLIDRDGIVRWANIECAEGLAGLGKSPSVDEILAVARALPGG"
BASE COUNT          856 a         1731 c         1766 g          938 t
ORIGIN      
        1 cgcccacctt gctattgccg ggatggtggg gcatatcggc gccatcggag acgtggccga
       61 gcggctgaag gcggcggttt gctaaaccgt tatacgctcc aaaggcgtat cgagggttcg
      121 aatccctccg tctccgccag aaatcagttt gtccaacccg gagacatggg taacggattg
      181 tcgtcagtgt ccgccggcag gttggcgaca tagtatttct tctctcccga gtttctgtgt
      241 tcgccgatca accaggcctc gtcgccgggc agatgttgct gacccttgtc cctgatccgc
      301 tgcgacggtc cgtcggcaac ccgcacgcgg accgcagcaa aacgagcctt gagccttttc
      361 ttttcgtgcc ggtacgccaa ctgatggtgc gctatctggc gttagccagc atgtcttcgg
      421 ccggtatcga taagatgtcc gggacctgcc gtcggcggga acgaccgccc cgggtaaccc
      481 cggccagatc atcttcacgg cggctgggta aaccttgagg tgccgcggaa tacccaccgc
      541 ccagatcagt ttgcgagcgg tgagccccgg cggaacggcg cacttagccc ataaccgcta
      601 tccgccagca cgcagccgaa ccgcaggcgc atccgagctg atctccccgg agcgcagcgc
      661 ggtgccatcg gttatcattt tctcccccga ggccgtgagg gaatggccat ctagccagag
      721 agtcgaccac ctgcggccgg tcagggttcg ataatcctca caaacgcgtt gtgaccccca
      781 tgaccgcgaa cagttgccac actctgccac cgtaactcct aaagacactt tccagcattg
      841 gccatgtttg agctgatcga gaaccgtagc gcacgtacag gtccttcggc atcggatctc
      901 cgcacgcttc ataggttgct caaatgggac gctgcggggc cagaacagtg ctatatggta
      961 tcccgcttcg ccacagtctg acgatccaac gcgcgatggc atgcagcggc tgcggtttcg
     1021 cagaactgtc accgggcttc gccttttgcc cggcgtgtgg gcgccggcag ccgggtctat
     1081 gcccggcgtg cgggttttcg tgcgaggccg atttcgcgtt ctgtccgcgg tgcggagcgg
     1141 cccggggcac aggttccccc ggggcggtac ctggcggaac tggagcaacg gccctggagc
     1201 cgcccccagt ccaggcgatc agagccgtgc cagcagcatc gccgggggcc ctcgaccggg
     1261 aggcggaccg ccggcaggtg acgatcctgt tcgccgacct gtccggattt accacgctct
     1321 ccgaacggct cgatccggaa gaggtgcgcg cgcttcagac agcgctgttc gagaccctcg
     1381 cccaggctat cactcgccac ggtggctccg tggtcaaatt catcggcgat gccgtcctgg
     1441 ccgtgttcgg cgcgccggtc gcgcacgagg acgatccgga gcgggcgctc gacaccgcgc
     1501 tcgaaatgct ggcgcgcggc gcgacactaa gcgatgcgtg gaccgcgcgt cttggccaac
     1561 cggtgatgtt gcacatcgct gtccacaccg ggccggtcgt ggcgggcagt ctcgctgcgg
     1621 cggccggcgc ggccggcgcg gtgtacgacg tgaccggtga caccgtgaac acgacgtccc
     1681 gtctgctcgg cgcggcaggt ccgggaacga tcctggtttc cgatgctacc catgcgctcg
     1741 cacggcatcg cttcgccttc gagccggccg gcgagttgac attgcgcggc aaggccgagc
     1801 cgattgccgt ccatcacctg ctcggcgcgc tcgcagaacc gggatcggcg cggaggctct
     1861 cccacgggct tgccgccccg atggtggggc gcttggacga actcgaacag ctcctcgctg
     1921 cgttcgaccg gatgcagtgg gggcgagccc aggtcgtgac cctgatcggc gaggcgggca
     1981 cgggcaagtc gcggctgatc gctgagatgc tggggcgggt cgaggccgat ggccgacttg
     2041 ccggaaccat cgtccgccgc gccgcctgct cctccctggg cgagcccacc tacgggatat
     2101 tcggggcgct gttccgcgaa gcctatcagg tggaagcgaa cgactcgctc gacatcgccc
     2161 ggcagaagct ggccgcaggg ctgcagtcgc tcggagcccg gcccgaggtg gtggagacca
     2221 ttgcgccggt gctgagctac atgctcggcg tggaggagac cctgccccgc gacggcgagc
     2281 ccgagcagct caagcggcag atcatgctgg ccgggcgaac gctggttgag ccgcacctgc
     2341 agcgcgggcc gctcctcatc atcgtggagg atctccactg gggcgacgtc gcgtcggtcg
     2401 atctgctgcg cgacgtcgtc gatcatctgt cggaccggcg gttgatgatg ttgttcacgc
     2461 atcgctccga catgcgtccg cccgtggtcg cgcgggccgc ccagtccatc atccggctct
     2521 ccccgctttc catcgacgag acgcggacgc tcgtggacgg cctgctcggt tctgtggccg
     2581 gggatgctat cgcccgggtg caggatttcg tcgcgacacg agccgggggc aatccgctgt
     2641 tcgtcgagga gatcgtccgc agcctgatgg ggaagggcgt gctggtccga cagggcgatc
     2701 actggacgtg cacggcggcc tgccaggcga tcgagattcc agccacgctg cacgggctgc
     2761 tgctttcccg catcgaccgg ctacccgccg acgctcggcg cctgcttcag gaggcggctg
     2821 tgctgggagc ggtgttcgac ctggctctgc tacgatccat tgcggttggc agcgaagccg
     2881 ccgaggccgc gttcgcgcgc ctcgccgagg ccgacctcat tcagcagatt ggacatgact
     2941 gcagtcacta ccggttcacg cacgcgctcg tgcatgaggc cgtctatcag aatctcctgc
     3001 tcgcgcgccg gaccgatctg cacgagcgga ccggccgcgc gctcgagcgc gccgccggtc
     3061 ctcacccgga gcgattgagc gatctggaag cgctcggcca ccattggagc ctctcgcccg
     3121 acaagcccag aggcgcacgc tacctcatgg ctgccggcga ttgggcgcgg tctgtctatg
     3181 cgaacgacga tgcaatccgc cattacgagc gcgcgttgcg caccctcgcc gattgccaga
     3241 cctgcgacga tcaggtgcag gcggtgcggg agcggctggc ggatctgctg acgctcaccg
     3301 gccggcgggc cgaggcgttg acgcactacg aggcggtgtg gcaggtggtc gagagtgccg
     3361 cggaccgcgc gggtgctgcg cgcctgcatc ggaagatcgg tggactgcac tgggaggccg
     3421 gtgatcgcga gcgcgccagc gcctgctttg cggccggtct cgatcggctc ggcgatgacg
     3481 gcaacccgat cgagcgcgcg cagctgttcc aggagatggg gcgacttgct tttcgtgccg
     3541 gcgacaacgc cgccgccatc gcatgggcgg agcgggcgct cgccgtggct gcgagcgggg
     3601 aggacgcgag cgatcccgag cgcgcccgcg aggcggccgc gacacgagcg cacgcctaca
     3661 acacgctagg ggtcgccctc gcgcgcatcg gccgcctcgc cgaggcggtc gaccagatcg
     3721 agcagagcat cgggctggcc gaggtgcaca atctgctgca agcgtcctgc cgcggttatg
     3781 cgaacctcgg cgtgctctac agctcgctcg atccgcagcg cagcatcgag acctgcttgc
     3841 ggggtctcga cacggcaaaa aaggtgggcg atctcggctt ccagtcgcgg ctctatgcaa
     3901 acctggccgt cgcctactgc gcgctgacgg atcggtgcga agcggaaggt atcgaggcag
     3961 cgcaagcggc cgtcgacctc gaccgtcggt tgggtttgct cgatcatctg gcggtgccat
     4021 tgatcgtcct cggtcagatc taccagtgcc acggcgacca cgccctcgca ttcgcgtcgt
     4081 atgaggaggc actcggtctg gccgagcaag tcggagagcc gcagcttctc tttccctgct
     4141 acgacggact cgctaccctt catctcgatg ctgggaacca agtgatggcg gaggcctatc
     4201 tggcgaaatc acaggcggtg tgcgagcggg ccgggatcga gcccgatgcc cttatggttt
     4261 tgccgtttct ctcctgacgc ggccgaggga ggaacttcca agatcatgac ccacgcgcca
     4321 cccccagtct cgccgggcga gccggcaccc gatttcaccc tttcaaccgt ggacggcgag
     4381 gaattggtat cgctggcgaa ctatcggggg agtcccctgt tcctcgctct cctccttggt
     4441 ctgtggtgcc cgttttgccg acggcagatc gcgcaactcg gtacgttcga gccgaaactc
     4501 aaagccgtgg gggtcgagtc gctcggagtc gtggcgacgc agccagacaa tgcccgtctg
     4561 tacttcagat tccggcccac gcggttgcgg ctggctgccg accccgacct tagcacgcac
     4621 cgcgcctacg gtgtgccgaa gccggtcccg acgccggaat tcatgcacgc gctggagacc
     4681 atacggatca atcccgacgg ggcgttccct gtgccgctgc caatcgtgga agcggcggca
     4741 acggttgcgg agctcgacgg ctacgtgaaa aacgagaccg accgcgccga catgcagcga
     4801 caatggcctc agctcaaggc ccattatctg atcgaccgtg acggcatcgt tcgctgggcc
     4861 aacatcgagt gcgcagaagg cctggccggg ctcggaaaat cgccctccgt cgacgaaatt
     4921 ctcgccgtgg cccgggccct gccgggcggc tgaccggggg cgtaaacggg gtggtcatga
     4981 gtgatgtcct gctcgagaaa gcaggacaga attgcgaatt tccaatcagg gcaggcgagc
     5041 cactgagctt gctaggagcc cgttccgcgt agacggcacc tgcggcccgc cacgaaagcc
     5101 ccgccccgtt ggcggggctt tttgctcggg tcaagcctcc acgacgcttt tagacggaag
     5161 ttacccccat tgaagatgca gaagtagctg atctgactcg cggattggca ggttcggctg
     5221 ttttgttact ggctacagtt tgacgggcac gtcgaggagt tggaacgcgc ggtcctgggc
     5281 gggtgtcaat t
//
LOCUS       DASRJQ010000086         3576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1069858, whole genome shotgun sequence.
ACCESSION   DASRJQ010000086 DASRJQ010000000
VERSION     DASRJQ010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069858"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..1116
                     /locus_tag="VFB89_01990"
     CDS             19..1116
                     /locus_tag="VFB89_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZM26102.1"
                     /translation="MFRFHVVQAEFGDCLMLEYGSPAEPRFTLIDGGPPNNYDDNLAP
                     VLRDIAKQNTPLDLAILSHVDNDHIVGLVDYFAELQAGAAGLPRPKELWHNSWGRAID
                     PQGVIAPRIVSLMTLNRTAAMPTATNAVLGIKEGNSLRTKAKQLNIPINAAFPNGDLI
                     TVGGAGQPVQLGNLRLQVVGPTQKNLDRLRKDWLAWIDKHEDDVGSDDVTIMANSDRS
                     VPNLSSIMLVAEADGKSILLTGDGRSDHLLEGLEEAGRLDGNGKLHVDILKLAHHGSE
                     RDVTREFFERVTADTYVASANGRDGNPDLPTLEWIADTAKESGRKISIVVTNRTPSVT
                     QFEKDFPAGKYTYRLTLMPKGDHVMVLTLAA"
     gene            1150..2571
                     /locus_tag="VFB89_01995"
     CDS             1150..2571
                     /locus_tag="VFB89_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26103.1"
                     /translation="MRVLGMGLDWKGQRLFAPIEEAAFTEALLARLAPNTARVRSVSK
                     AANRGAVFRGEAARRTIDPGDPKAAGWSFLVAENDPRKGAIVEALAPLAKARGMADPA
                     KPLLFASSGPDDWQRWLTERYYALDLTNQKVPGYVLIIGGPEQVPFGFQSLMDSVASV
                     GRLEFERIEDLGVYVDKLLRLEAAPDPIVEREVLFFATDGGLEDPTYFSRKYMAEPLA
                     AHVRDEHKFKTTEIMGEEATKANLLKALKQTKAGLVYTASHGLGATSETMAVQTRYNG
                     AICCQSDGPLTLDHLLSADDIPLSEPILEGAVVFQFACFGYGTPAVSEYAHWMPETSG
                     KTEQLAERDFIAALPKRLLAHPRGPIAFIGHLDTAFLHGFADASQPHTLDRWHARIAP
                     FKKAVDRLLGVQPSGLAMEGINERYALCNAMITNTNDRVQRGTMTWNEEALAGFLDTW
                     ITRGDAQNYLIFGDPAARLRLPA"
     gene            2575..>3576
                     /locus_tag="VFB89_02000"
     CDS             2575..>3576
                     /locus_tag="VFB89_02000"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HZM26104.1"
                     /translation="MDPALRYINLDLWVERGAEGVYTVRGMAPQGDVRDAATEDPAHL
                     GANDEKLSGNTADAEYIKALGIRQYRFAFRTAQGRIQSLLDRCVGAGGEDCGVRIRLR
                     HADVNPEISAIPWEFAHDGSDDGGFLASSERTPIVRFVESEKPPRSPEARLPLNMLVV
                     IPTVPDLDVDGEKRRIREALEGIDPPVAVTFLEGKVTRERLSDALQNESIDFLHFVGH
                     GDVSEGKGRLRLNLSELEPDWIDEAAMAELVRNRRALKLIVLNTCQGAAPSTSHAFAG
                     VAPQLVLAGVPAVVAMQFPITDKEALTFVRAFYHALFQGTVRGCVDGATTAARSALKR
                     "
BASE COUNT          654 a         1179 c         1155 g          588 t
ORIGIN      
        1 tgaccaggaa gtgatgccat gtttcgattc catgtggtcc aggcggaatt cggcgattgc
       61 ctgatgctgg aatacggatc gccggcggag ccgcgcttca cgctcatcga cggcgggccc
      121 ccgaacaatt acgacgacaa cctggcgccg gtgctcaggg acatcgcgaa gcagaacacc
      181 ccgctcgacc tcgccatact gagccacgtc gacaacgacc atatcgttgg actggtggac
      241 tacttcgccg agcttcaggc cggcgccgca ggcctcccgc gtcccaagga gctctggcac
      301 aactcctggg gacgcgccat cgatccccag ggagtcatcg caccacggat cgtgtcgctc
      361 atgacgctca accggaccgc cgcgatgccc accgccacca acgcggtgct cggcatcaag
      421 gaagggaatt cgctccggac caaggccaag cagctgaata tcccgatcaa cgcggcgttt
      481 ccgaacgggg acctgatcac ggtcgggggt gccggacagc cggtgcagct cggcaacctt
      541 cgcctgcaag tcgtgggtcc cacccagaag aacctcgacc ggctccgtaa agactggctc
      601 gcctggatcg acaagcacga ggatgacgtc ggcagcgacg atgtcaccat catggccaac
      661 tcggatcgtt cggttcccaa tctgagcagc atcatgctgg tcgcggaagc cgatggcaaa
      721 tccattctcc tgactggaga cggccggagc gatcatctgc tggaggggct cgaggaggcc
      781 ggccggctgg acggcaacgg caagctccac gtcgatatcc tcaagctggc tcaccacggc
      841 agcgagcggg atgtgacgcg agagttcttc gagcgggtta cggcggacac ctacgtcgcc
      901 tcggccaacg ggagggacgg caaccctgat cttcctactc tcgagtggat tgccgacacg
      961 gcgaaggaga gcggccgcaa gatcagcatc gtcgtcacga accggacgcc gtcggtgacg
     1021 cagttcgaga aggacttccc cgccggcaag tacacctatc gccttaccct catgcccaag
     1081 ggcgatcacg tcatggtgct gacgctcgcg gcgtgatcat tcggttggat cgacggagga
     1141 ggcttgacga tgcgtgtgct cggtatggga ctggactgga aaggccagcg tctcttcgcg
     1201 ccgatcgagg aggctgcctt taccgaggca ctcctcgccc ggctcgcccc caacacggcc
     1261 cgagtccgca gcgtctcgaa ggccgccaac cgcggggctg tgtttcgggg cgaggccgcc
     1321 aggcggacca tcgacccggg ggatcccaag gccgcggggt ggagctttct cgtcgcggag
     1381 aacgatcccc gaaagggtgc catcgtggag gccctggcgc cgctcgccaa ggcgcgcggc
     1441 atggcggacc ccgccaagcc gctgctgttt gccagcagcg gccccgatga ctggcagcga
     1501 tggttgacgg aacggtacta cgcgctcgac ctgaccaatc agaaggtgcc cggctacgtg
     1561 ctcatcatcg gcgggccgga gcaggttccc ttcgggttcc agtcactcat ggactcggtc
     1621 gcgagcgtag ggcggctgga gttcgagcgc atcgaagact tgggggtgta cgtcgacaag
     1681 ctcctccggc tggaagcggc acccgacccc atcgtggagc gggaggtgct cttcttcgcg
     1741 acggacggcg gactcgagga cccgacctac ttcagcagga aatacatggc cgagccgctg
     1801 gccgcccacg ttcgcgacga gcacaaattc aagacgaccg agatcatggg cgaggaggct
     1861 accaaggcca acctcctcaa ggcgctcaag cagaccaagg ctggactggt ctacaccgcg
     1921 agccatggtc tgggcgccac ctcggagacc atggcggtcc agacgcgcta caacggcgcc
     1981 atctgctgcc agtcggacgg cccgctcacc ctcgatcacc tgctctctgc ggacgacatt
     2041 ccgctgtccg agccgatcct cgagggcgcg gtggtctttc aattcgcgtg cttcggctac
     2101 ggcaccccgg ccgtgagcga gtacgcgcac tggatgccgg agacgtcggg caagacggag
     2161 cagctcgccg agcgtgattt catcgccgcg cttccgaagc ggttactggc ccacccccgc
     2221 ggcccgatcg cgttcatcgg ccacctcgat acggcattcc tgcacggctt tgcggacgcc
     2281 tcgcagccgc acacgctcga tcgctggcat gcgcgcattg ccccgttcaa gaaagcggtg
     2341 gaccggcttc tgggggtcca gccctcgggc ctggccatgg aggggatcaa cgagcgctat
     2401 gcactctgca atgcgatgat caccaacacc aatgatcgcg ttcagcgggg gacgatgacc
     2461 tggaacgagg aggcgctggc cggcttcctc gatacctgga tcactcgggg cgacgcgcag
     2521 aactatctga tattcgggga tcctgccgca cgtctccgcc tgccggcgta ggcagtggac
     2581 ccggcgctcc gttacataaa cctcgacctc tgggttgagc gaggagccga aggtgtctac
     2641 accgtccgcg gcatggcgcc ccagggcgac gtgcgggacg ccgcgaccga ggatcccgcc
     2701 cacctcggcg ccaacgacga gaagctctcc ggcaacaccg ccgacgccga atacatcaag
     2761 gcgctcggga tccgtcagta tcgcttcgcg tttcgaaccg cacagggcag gattcagagc
     2821 ctgctggatc gctgtgtcgg ggcgggtggg gaagactgcg gcgtccggat tcgcctccgg
     2881 cacgcggacg tcaatcccga gatctcggca atcccgtggg agtttgccca cgatggatcc
     2941 gatgatggcg gattccttgc ctcctccgag cggacaccca tcgtccgttt cgtcgaatcg
     3001 gagaagccgc cccggtcccc ggaagcccgg ttaccactca acatgctggt ggtcattccg
     3061 acggttccgg atctggacgt ggacggcgag aagcgccgca tcagggaggc gctcgaggga
     3121 atcgatcctc cggtggccgt cacctttctg gagggcaagg tcacccggga gcggctgagc
     3181 gatgccttgc agaacgagag catcgatttt ctccacttcg tcgggcatgg ggatgtctcc
     3241 gagggaaagg gccgcctccg gctgaacctt tccgagctgg agcccgactg gatcgacgag
     3301 gcggcgatgg ccgagctcgt caggaatcgc cgtgccctca agctcatcgt cctgaatacc
     3361 tgccagggcg ccgcgccctc cacctcccac gccttcgccg gggtggcacc ccaactggtc
     3421 ctggccgggg tacccgcggt cgtggcgatg cagttcccga tcaccgacaa agaggcgctc
     3481 acattcgtcc gcgcgttcta ccatgccttg ttccagggga ccgtgcgggg ctgcgtcgat
     3541 ggggcgacta ccgcggcgcg cagtgcgctg aagcgg
//
LOCUS       DASRJQ010000087         4551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3884864, whole genome shotgun sequence.
ACCESSION   DASRJQ010000087 DASRJQ010000000
VERSION     DASRJQ010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3884864"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..1062)
                     /gene="dusB"
                     /locus_tag="VFB89_02005"
     CDS             complement(79..1062)
                     /gene="dusB"
                     /locus_tag="VFB89_02005"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HZM26105.1"
                     /translation="MQLPYPSEHSFGLILAPMAGVSEAPFRQVCRGMGADVVLSEFLS
                     SEAIRRRIRNTLEGAEFEECERPIGIQIYGSDPNAMAEAAGLITEHYGPEFIDINFGC
                     PVKKVVQRNGGSGCLRDLDQVDRIIRAVRGATHLPVTVKTRSGWNDQLRDPVGIARRM
                     QDSGAEAFTLHARTRTQMFSGRADWDEIARVVEALDIPVIGNGDIQSPEDVVRMRDHT
                     GCAGAMIGRGAFGNPWLFRDAQALLAGRPKPPVPDATERFRVALEHARLALRLQGDTR
                     KTVIEFRKHLGWYTKGLHGASDLRQRLFQIASIAEAEAIFLEYLDPVAQVA"
     gene            1117..1569
                     /locus_tag="VFB89_02010"
     CDS             1117..1569
                     /locus_tag="VFB89_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HZM26106.1"
                     /translation="MRLRDFFAPGTVRLALQSATRDDVLRELVGLLDVSEVSAETVLR
                     ALQRRESLGSTGVGRGVAIPHCRSAVVNRVRLAYGHRAEGIEYQSIDGKPVQDFFLIV
                     APPDEAVNMYLPVLGRIAQFAKEADVPARLATLTDPDQFLALLDEKGV"
     gene            1658..2233
                     /gene="msrA"
                     /locus_tag="VFB89_02015"
     CDS             1658..2233
                     /gene="msrA"
                     /locus_tag="VFB89_02015"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HZM26107.1"
                     /translation="MPSKQFETATFAGGCFWCLEAVFERLMGVERVVSGYTGGSTPNP
                     TYEDVSTGRTGHAEVVQVTFDPTAISYRELVEIFFAFHDPTTLNRQGPDTGTQYRSAI
                     FFGNEEQAATAREVIAELEAKRLFGAPIVTEVTPLGAFYPAEEYHQQYFQRHPERMYC
                     QAMIAPKVAKLRKAYSSRLREPVAASPSARP"
     gene            complement(2193..2624)
                     /locus_tag="VFB89_02020"
     CDS             complement(2193..2624)
                     /locus_tag="VFB89_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26108.1"
                     /translation="MPDPLTRLLRAILPKATAQEVAEAAERLATLIESGATWVGDTAG
                     ETVRKVESVLSGGDVTPRAMPALPTTVEDLVWLKVTRALAPRVGEIQARARALRSEPD
                     LREDIVALTQDVDWLVGELGSAWAREVALRAARTETPPPAP"
     gene            complement(2631..4187)
                     /gene="guaA"
                     /locus_tag="VFB89_02025"
     CDS             complement(2631..4187)
                     /gene="guaA"
                     /locus_tag="VFB89_02025"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HZM26109.1"
                     /translation="MTEHHEGILIIDFGSQYTQLIARRVREAHVYCEIHPPSRTVAWI
                     REWKPKGIILSGGPNSVYDENVPTADAELLETGLPILGLCYGMQLLAHLSGGKVIRAN
                     RREYGRADVKVRGGRLFQGFGSGEETPVWMSHGDHVDTPPPGYCVTASSDNSPVAAFE
                     HGELPLFGVQFHPEVAHTPRGGEMLNTFLFDICGCTPDWTPGHFIEMEIERIRKLVGD
                     RERVICGLSGGVDSSVAAVLVHRAIGDCLTCIFVDHGLLRLHERDQVETTFRRHLGID
                     LRVVDAANLFLERLAGVAEPEEKRRRIGHTFIEVFEAEARKVGSDVGWLVQGTLYPDV
                     IESLSPRGGPSVTIKTHHNVGGLPERLPFRLLEPLRELFKDEVRQVGRELGMPEEMVG
                     RHPFPGPGLAIRILGPVERNSLDVLRRADQIYIEEIRAAGLYDSIWQAFAVLLPIRSV
                     GVQGDGRTYDQVVALRAVTSRDGMTADWFPFPPDVLARAANRIANEVDGVNRVVYDVS
                     SKPPATIEWE"
     gene            complement(4184..>4551)
                     /locus_tag="VFB89_02030"
     CDS             complement(4184..>4551)
                     /locus_tag="VFB89_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HZM26110.1"
                     /translation="VIVDAGMNDLVRPSHYHAYHELVELEARGGRTGAVDVVGPVCET
                     GDFLALDRELPALERGDRLAVLGAGAYGFVMSSNYNSRPRAAEAIVDGDRWWISRRRE
                     TIGDLFTGERATPERADSA"
BASE COUNT          832 a         1491 c         1426 g          802 t
ORIGIN      
        1 gcagccgact gacggcagtc tccggtgaga ggcgacctgc ggcgacttca tgaagcagtt
       61 cttccagctg agcgggagtc acgccacctg agcgaccggg tcgaggtact cgaggaagat
      121 ggcttcggct tcggcgatcg acgcgatctg aaagagacgc tggcgcagat cgctggcacc
      181 gtggagccct ttggtgtacc acccgaggtg cttgcggaac tcgatcaccg tcttgcgagt
      241 gtcgccctgt agccgaagag ccaggcgcgc gtgctccagc gccactcgga agcgctcggt
      301 tgcgtcgggc acaggaggct tgggccgtcc ggcgagcagc gcctgtgcat cacgaaacag
      361 ccagggattg ccgaaggcac cgcgcccgat catggcgcca gcgcagccgg tgtggtcccg
      421 catccgaacc acgtcctcgg ggctctggat gtcgccgttg ccgatcaccg gaatgtccag
      481 cgcttccacc acccgcgcga tttcatccca atcggcacgg cccgaaaaca tctgggtccg
      541 ggtgcgggcg tgtagcgtga aggcctcggc cccggaatcc tgcatccgac gggcaatccc
      601 gacaggatcg cgaagctggt cgttccatcc gctgcgagtc ttgacggtga ccgggaggtg
      661 agtggcccca cgcaccgcac gaatgatccg atccacctga tcgaggtcgc gcaggcagcc
      721 cgacccgccg tttcgctgca ccactttctt caccgggcag ccgaagttga tatcgatgaa
      781 ctcgggcccg taatgctcgg tgatgagccc ggcggcttcg gccatcgcat tgggatccga
      841 cccgtagatc tgaatgccga tgggccgctc acattcctcg aattcggcgc cctccagcgt
      901 attgcgaatg cggcgacgaa tggcttcgga cgacagaaac tcggacagca caacgtcggc
      961 tcccattccg cgacagacct ggcggaacgg ggcctccgag acgccggcca tcggcgctag
     1021 aatcagcccg aacgaatgtt cggagggata gggcaactgc atggggtgaa aatacttgtt
     1081 gacacgtccc gcggcgaggg ggtagcttcg ggcgccatgc gcctcagaga cttcttcgca
     1141 ccgggaacgg tgcgtctcgc gctccagagc gccactcgcg acgacgtcct gcgcgagctg
     1201 gtcggactgc ttgacgtgag cgaagtatca gcggagacgg tgctgcgcgc gctgcaacgg
     1261 cgtgagagtc ttggctccac tggagtggga cgcggcgttg cgatccccca ttgccgatcg
     1321 gccgtcgtca accgcgttcg gctggcctac ggccaccgag cggagggcat cgagtatcag
     1381 tcgatcgatg gcaagccggt gcaggatttc ttcctgatcg tcgctccgcc cgacgaagcc
     1441 gtgaacatgt acctccccgt gctcgggcgc atcgctcagt tcgccaagga agcggacgtc
     1501 ccggcgcgcc tggccacgct caccgacccg gaccagtttc tggcgttact ggacgaaaag
     1561 ggcgtatagg atcgcccgcg gctcgaattc aaccgcaggg aaccgaggtg cccctccggg
     1621 cgtttcttcg gcggcaactc ggaaaatcgg aggaaagatg ccttccaagc agtttgagac
     1681 cgccactttc gccggcggct gtttttggtg tctggaagcc gtgttcgaac ggctgatggg
     1741 agtagagcga gtggtctccg ggtacaccgg tggctcgacc ccgaacccta catacgagga
     1801 cgtgagtacc ggcaggaccg gccacgcgga agtggttcag gtcacgttcg atcccacagc
     1861 gatttcgtat cgcgagctgg tggagatttt cttcgcattc cacgatccta ccacgctcaa
     1921 ccgccaggga cccgacaccg gtacccagta tcgctctgca attttctttg ggaacgagga
     1981 acaggcggcc actgcgcgtg aggtgatcgc ggaactcgag gcgaagcggc tgttcggtgc
     2041 cccgatcgtc acggaggtca cgccgttggg cgccttctac ccagcggagg agtatcacca
     2101 gcagtatttc cagcggcacc ccgagcggat gtactgtcag gcaatgatcg ccccgaaggt
     2161 ggcgaagctg cgtaaagcgt actcgtcccg tctcagggag ccggtggcgg cgtctccgtc
     2221 cgcgcggccc tgagcgccac ctcgcgggcc caggccgagc ccagctcacc caccagccaa
     2281 tcgacatcct gcgtgagggc tacgatatcc tcacgcagat ccggctcgga ccggagagcg
     2341 cgggcccgcg cttgaatctc gccgacccga ggcgccagag cccgggtgac tttgagccaa
     2401 accaggtctt caaccgtcgt cggcaaggcc ggcattgctc gcggggtaac gtcccctcca
     2461 ctcaggacgc tctccacttt ccgaactgtc tctcccgcag tatcgcccac ccacgtggcg
     2521 ccggattcga tcagcgtagc gagccgctcg gccgcttccg ctacctcctg agccgttgct
     2581 ttgggcagga tggcacgcag cagtcgagtc agcggatccg gcatctctgg tcactcccac
     2641 tcgatggtcg cgggcggctt cgagctgacg tcgtacacca cccggttgac gccgtccact
     2701 tcattggcaa tccgattggc tgctcgcgcg agcacgtcgg gagggaatgg gaaccagtcg
     2761 gctgtcatcc cgtcccggct cgtgactgcg cgaagggcca cgacctgatc gtaggtgcgt
     2821 ccgtctccct gaactccgac cgaccggatc ggcagcagca cggcgaacgc ctgccagatg
     2881 gagtcataca gaccggccgc tcggatttcc tcgatataga tctgatcggc gcgccggaga
     2941 acgtccaagc tgtttcgttc caccggaccg aggatgcgaa tggccagtcc cgggccggga
     3001 aacgggtggc ggccgaccat ctcctcgggc atccccagct cgcggcccac ctgacgcacc
     3061 tcatccttga acagctcgcg cagtggctcg agcaggcgga acggcagtcg ctcggggagg
     3121 ccgcccacgt tgtgatgtgt tttgatcgta accgacggtc ctccacgagg cgatagcgat
     3181 tcgatcacgt cgggatacag cgtcccctgc accagccagc ccacgtcgga gccgaccttg
     3241 cgcgcctcgg cctcgaacac ctcgatgaac gtgtgcccga tccgacggcg tttttcctcc
     3301 ggctcggcaa caccggccag gcgctcgaga aagagattcg cggcgtcgac cacgcgaaga
     3361 tcgatgccca ggtggcggcg gaacgtggtt tcgacctggt ctcgctcatg gagccggagc
     3421 agtccgtgat cgacgaaaat gcaggtgaga cagtcgccta tcgcgcggtg aaccagcacg
     3481 gcggcaaccg atgagtcaac tccacctgat aacccacaga tgacccgctc gcggtcgccc
     3541 accagcttgc gaattcgctc gatttccatc tcgatgaagt gacccggcgt ccagtcgggc
     3601 gtgcagccgc agatgtcgaa gaggaaggtg ttgagcatct cgccgccccg aggcgtatgc
     3661 gcgacctcgg ggtgaaactg gaccccgaac agcggcagct ccccgtgctc gaatgcggcg
     3721 acgggcgagt tatcgctcga tgccgtgacg cagtagcccg gcggcggcgt atcgacatga
     3781 tcgccatgac tcatccagac cggcgtctct tcaccgcttc caaacccttg aaagagccga
     3841 ccgccccgga ctttcacgtc tgctcgcccg tattcccgcc ggttggcgcg aatcaccttg
     3901 ccgcccgaca agtgagccag aagctgcatt ccgtagcaaa ggcccagaat tggcagaccg
     3961 gtctcgagca gctcggcatc cgcggtgggt acattctcgt catagaccga gttgggtccg
     4021 cccgaaagga tgatgccttt gggcttccac tcacggatcc aggcgaccgt ccgcgagggc
     4081 ggatggattt cgcagtagac gtgcgcctcg cgtacccgac gcgcgatcag ctgggtgtat
     4141 tgcgatccga aatcgatgat cagaatgcct tcgtgatgct cggtcacgct gagtcggccc
     4201 tttccggtgt ggcacgctct ccagtgaaca gatctccgat ggtttcccgg cgccgcgaaa
     4261 tccaccagcg gtcgccatcg acgatggcct cggcggcgcg ggggcgggaa ttgtaattcg
     4321 agctcatgac aaagccgtat gccccggctc ccagaacggc aaggcgatcg ccgcgctcga
     4381 gtgccggcag ctctcggtcg agggcaagaa aatccccggt ttcgcacacg ggtcccacga
     4441 cgtccacggc accggtccgt ccccctcgtg cctcgagctc gaccagttcg tgatatgcat
     4501 gatagtggct cggccgaacc aggtcgttca tgcccgcatc cacaattacc a
//
LOCUS       DASRJQ010000088         2997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3323628, whole genome shotgun sequence.
ACCESSION   DASRJQ010000088 DASRJQ010000000
VERSION     DASRJQ010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3323628"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VFB89_02035"
     CDS             complement(<1..543)
                     /locus_tag="VFB89_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26111.1"
                     /translation="MITCRVLGPVEVTVDGAAAPPELLWRKHLALLLYLARSPKRART
                     REHLTGLLWPETTDSAARHSLNEALRVLRRSAGDEALDTSGGQIRLSADAVRLDADQL
                     DGWIASENWDALGGAIAGEFLEGFSVAGSDGLEDWLMNERRYWNGRAATALLGWTNAL
                     LSEGRADEALAVARRAQALEP"
     gene            complement(540..>2997)
                     /locus_tag="VFB89_02040"
     CDS             complement(540..>2997)
                     /locus_tag="VFB89_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM26112.1"
                     /translation="DFTRDLLAALGTAHAQGVVHRDIKPANIFLRQGQALLADFGVAS
                     WRSDSDGLTTPGQMIGTLRYMPPEARDGSEVTGSADVYAAGMVIYEACAGVHWPAYQV
                     PGEADWRMVPRVLVAPLKRALAIAPEERWPDGNAFRQVVTPRQRSLAGPLLAAGVVLA
                     AIAAAIWLVPRGQPTAPVVARTGVTLALARIAVQGQPGRSGLGDSVRTALVDALSGFP
                     DFSVLPGSSGGDSLAFVITGTGNSDGSALSLQLTMRAPHAQSIQFPIKGREEDWHGLT
                     DTLVNRLARAIWQRVEGGNRDLPLAAIPRSDNGFSLWLKAEQYLSQARWDEADTAYTT
                     AEHEDSTCYLCWFRRLDIDRWVGPPQDSAGLALLGRVRDSFPARYQSLINAALTPLPW
                     RLDTLRVAAETYPTFFLASFEYGDELFHRGPLYGHPRHEAIEPLHKTVQLRPRFGPGW
                     EHLTWALLSEGDSASTVAALDSLEATKVGSGLSAGLRMLLTLGVHWRFFSPDSAARFS
                     SAVLHNLGVLRNPDAVAGARLLMTTDAPNGAVAFGSLLTTMRAWRTEAVGEGLLGQMY
                     GYAALGKLDSLRAVGLRLSRETTDPSLPLLALELEAILRRFDPEPSVRTAPGLVEALR
                     VYLPRGAYDRPLRWRAGWALGLLASGTGDSALLATALGAVFDEPALSRMRRTLEASDL
                     AARNKLRDALRALPDTIGYDVLPGDHESPLDDTAARLLAAEWAERQIGRDSARKALLW
                     QEHLHHHGHLTGPPQRAEGSWAVSTLARWRLAQLFRKGEAARCAALASVERRWKQGDS
                     VFAARADSARTLIAQECKPS"
BASE COUNT          495 a         1010 c         1039 g          453 t
ORIGIN      
        1 gggttcgagt gcctgcgcgc ggcgcgcgac cgccagcgcc tcatcggcgc ggccctccga
       61 gagcagtgcg ttggtccatc cgagcagcgc cgtcgcggcg cgcccgttcc agtagcgccg
      121 ctcgttcatg agccagtcct cgagcccgtc cgagccggcc accgagaatc cttcgagaaa
      181 ctcgccggcg atggcgcccc ccagggcatc ccagttttcc gaggcgatcc agccgtcgag
      241 ctgatcggcg tcgagccgca ccgcgtcggc ggaaagccgg atctggccgc cggaggtgtc
      301 gagcgcttcg tcgccggcgg agcggcggag cacgcgcaga gcttcgttga gggaatgccg
      361 cgccgcgctg tcggtcgtct cgggccagag caggccagtg aggtgctcgc gggtacgggc
      421 gcgcttgggc gagcgggcga gataaagcag caatgcgagg tgcttgcgcc agagcagctc
      481 gggtggagcg gcggccccgt ccacggtcac ttccaccgga ccgagcactc ggcaggtgat
      541 cacgacggtt tgcactcctg cgcgatgagg gtgcgcgcac tgtctgcgcg tgcggcgaag
      601 accgaatcac cctgtttcca gcgccgctcc accgaggcca gcgcggcgca gcgggcggct
      661 tcccctttcc tgaagagttg cgccagtcgc catcgcgcca gcgtacttac tgcccaggag
      721 ccctcggcgc gttgcggcgg tccagtcaga tggccgtgat ggtgaaggtg ctcctgccag
      781 agcagtgcct tgcgggccga gtcgcggcct atctggcgct ctgcccattc ggcggccaga
      841 agccgggcgg ccgtatcgtc gagggggctt tcgtggtcgc ctggaagaac gtcgtagccg
      901 attgtgtcgg gcagggcgcg aagcgcatcg cggagtttgt tgcgggccgc gaggtccgac
      961 gcctcgagcg ttcgccgcat tcgactcaag gccggttcgt cgaacactgc ccccagcgcc
     1021 gtcgccagga gagccgagtc gccggttccg ctcgcgagta atccgagcgc ccagcccgcc
     1081 cgccaccgca aaggccgatc ataggcgccc cggggcaggt atactcgcag cgcctccacc
     1141 agacctgggg ctgtgcggac gctgggctcg ggatcgaagc gacgcaggat cgcttccagc
     1201 tcgagcgcaa ggagcggcag gctaggatca gtggtctccc ggctgagtcg caggccaacc
     1261 gcgcgcagcg agtcgagctt ccccaaggcc gcgtagccgt acatctgtcc gagcagtccc
     1321 tcacccactg cctcggtccg ccaggcccgc atggtggtta gcaagctgcc aaacgcgacg
     1381 gcgccattgg gcgcgtcggt cgtcatcagg aggcgggccc ctgcgaccgc atccgggttg
     1441 cggagaactc ccagattgtg gagcaccgcg ctggagaacc gcgcggcgct gtcgggagag
     1501 aagaacctcc aatgcacgcc aagcgtcagg agcatacgca gcccggcgga tagtcccgaa
     1561 ccaactttgg ttgcctcgag gctgtcgaga gcagcgaccg tggaagcgga gtcgccctcg
     1621 ctcaggagtg cccaggtgag gtgctcccag ccggggccga agcgcgggcg aagttgcacg
     1681 gtcttatgga gcggctcgat agcctcatgg cgaggatggc catacagcgg gccgcggtgg
     1741 aacagctcgt ccccgtactc gaacgaggcc aggaaaaagg tgggataggt ctccgccgct
     1801 actcggagag tatcgaggcg ccaggggagc ggggttaacg cggcattgat cagcgactgg
     1861 tatcgagccg ggaacgaatc gcggacccgg cctagcaggg caagaccagc cgagtcctgg
     1921 gggggcccca cccagcgatc gatgtccagg cgccgaaacc agcagagata gcaggtggag
     1981 tcctcatgct cagccgtggt gtacgcagtg tcggcttcgt cccaccgggc ctgggacagg
     2041 tactgctccg ccttgagcca gagcgagaag ccgttgtcgc tccgaggaat cgccgcgagc
     2101 ggaaggtctc ggttgcctcc ctccactcgc tgccagatcg cacgcgccaa gcggttgacg
     2161 agcgtatcag tcaggccgtg ccagtcctcc tctcttccct ttatggggaa ctgaatcgat
     2221 tgggcatggg gagctctcat cgtcaactgc agcgagagcg ctgatccgtc gctgtttccc
     2281 gtgcccgtaa tgacgaaagc gagcgagtca ccccctgagc tccccggcag cacggaaaaa
     2341 tcgggaaagc ccgagagcgc atcgacaagg gcggtccgca ccgagtcgcc caggccgctg
     2401 cggccgggct ggccctgcac cgcgatccgg gccagcgcca gcgtcacccc ggtgcgggct
     2461 acaacgggag cggtgggttg tcccctggga accagccaga tcgccgccgc gatagccgcg
     2521 agcacgacac ctgccgccag cagcggtcct gccaggcttc gctgcctggg cgtcacgacc
     2581 tgccggaacg cgttcccatc aggccagcgc tcttccggag cgatcgcgag cgcccgcttg
     2641 agcggcgcta ccagcacccg ggggaccatg cgccagtctg cctcgccggg aacctggtag
     2701 gccggccagt gcacaccggc gcacgcctcg tagatcacca ttcccgccgc gtacacgtcg
     2761 gccgacccgg taacctcgct gccgtcacgc gcttcggggg gcatgtagcg gagcgtaccg
     2821 atcatctggc ccggcgtggt gagcccgtcg ctgtccgacc gccagctcgc gacgccgaag
     2881 tcagccagga gtgcttgtcc ctggcggagg aagatgttgg ccggcttgat gtcgcggtgc
     2941 actactccct gggcatgcgc cgtacccagc gcggccaaca ggtcgcgggt aaagtcg
//
LOCUS       DASRJQ010000089         4940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3043531, whole genome shotgun sequence.
ACCESSION   DASRJQ010000089 DASRJQ010000000
VERSION     DASRJQ010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3043531"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..421
                     /gene="rpmE"
                     /locus_tag="VFB89_02045"
     CDS             203..421
                     /gene="rpmE"
                     /locus_tag="VFB89_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HZM26113.1"
                     /translation="MKANLHPVYRKITAVCACGNQFETRSTASSIHVEVCAQCHPFFT
                     GKQRLVDTAGRVDRFRRKYKSETAPAKA"
     gene            425..1483
                     /gene="prfA"
                     /locus_tag="VFB89_02050"
     CDS             425..1483
                     /gene="prfA"
                     /locus_tag="VFB89_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HZM26114.1"
                     /translation="MENRLRQALARAEEVAQALADPDVSRDPARLRSLGREHTRLVPV
                     VKAAERLARLRDELQQARELAGEADPELVALARADLNRLGPAIEDLQAELHELLLPRD
                     PHDDRDAIVEVRAGTGGDEAGLFAAELGRMYTRYAERHGLAVEPISVSEGNLGGVKEA
                     IFAVRGTGAYGLLRWESGVHRVQRVPQTETQGRIHTSAATVAVLPEAEEVDVKIEPQE
                     LKVDVFRSSGPGGQSVNTTDSAVRITHLPTGLVVQQQDQKSQLQNKIKAMEVLRARLL
                     DRMIAEQEAARARDRRAMVGTGDRSAKIRTYNFPQSRVTDHRINLSVHNLDDVMNGSL
                     EPFVNALRLASREEQVSA"
     gene            1476..2330
                     /gene="prmC"
                     /locus_tag="VFB89_02055"
     CDS             1476..2330
                     /gene="prmC"
                     /locus_tag="VFB89_02055"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HZM26115.1"
                     /translation="MPELTGSLASLLAEARLILGAAGIPEPAREALLLWADLSGEAAA
                     GVLLASSRTVDPDMATGFLAAIDRRTTGEPLAYVTGWTGFRLLKLRIDRRALIPRPET
                     EGLVELVLERVDEGRIVDVGTGSGCVALSLATEGRYKSVVAIDKSAAALRLAALNREL
                     VGASVALVRADLTTTLRDACVDALVSNPPYLTEQEYAGLDPAVSLWEPREALVSGRGG
                     MDATARLLDDGRRVVRAGGWIALELDATRAAMVAETAERRGWEAVEVRADLFGRERYL
                     LARRSKTP"
     gene            2327..2680
                     /locus_tag="VFB89_02060"
     CDS             2327..2680
                     /locus_tag="VFB89_02060"
                     /inference="COORDINATES: protein motif:HMM:NF017902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HZM26116.1"
                     /translation="MIWDKAQELGRLIGQTPEYQALRRAETNLREDQDTQAKLDQIQR
                     LARQVDQMVAQGQMPDQPTAEAYETAVRDLEMSGSGQAYVVARANFEKLMTKVNQQIS
                     EGMEKGATSTIITLS"
     gene            2677..3342
                     /gene="tmk"
                     /locus_tag="VFB89_02065"
     CDS             2677..3342
                     /gene="tmk"
                     /locus_tag="VFB89_02065"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HZM26117.1"
                     /translation="MNEGFFVVVEGPEGAGKSTLAAGLVRLMQELGWDPVGVREPGGT
                     PAAEAVRAAFLDPANRFAPHTELLYICAARAHLVQEVIQPALAAGRPVVSDRYELSTL
                     AYQGAGRGLDPDIVAFVNRVATAGLRPHLTLVLDIPAEVGLDRLKAGAKKHDRLEQED
                     PGFHRRVAAAYLAAIGDGVRHLNAVAPPAAVLDAAWAEVVAAAPATFRSGGDSRAVRR
                     TDV"
     gene            3339..>4940
                     /locus_tag="VFB89_02070"
     CDS             3339..>4940
                     /locus_tag="VFB89_02070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HZM26118.1"
                     /translation="MKQRWGLIGLVAVISFLSGGWLLQRGVAAGGNVYQQARLFDDVL
                     SHVHNYYVDSLGESDLYDKATNGMLEELKDPYSVLLTGDDYRALTEQTSGNYAGLGIQ
                     IDVRDGWITVVAPLPDTPAERAGIQTGDQIVEVDGKSTEGWKNDQAVKALRGEAGSKI
                     AVKVRRSGIADPITFDLTRATIHIRSVPPGTLFDGGIGYISLNPVAETSAQELRDEIT
                     SMKSKGMKSMILDLRLNPGGLLDQGVKVAELFLDKGQEIVSTKGRARGSSKEFYDEVK
                     QLWPDLPIVVLVNEGTASAAEIIAGALQDHDRAVVVGSPTFGKGLVQTLFPLGDGVAL
                     KLTTARWYTPSGRTIQRIAKDEEDQLAQAESAAAGTLTAESTDSALKERPIYRTDAGR
                     AVRGGGGIVPDLVIRPDTLTTGERTFAKALGAKLPVYRDVLTTYALELKRTKGVENES
                     FKISPEMRREVFQRLRNKGVELTQADFSAAGQLVDEQLGYEIERYVFGRPAEFRRRSL
                     DDRQLQSALGLLKKAQTPKDLMGIAM"
BASE COUNT          911 a         1485 c         1698 g          846 t
ORIGIN      
        1 gttagccagt atatgtagcc cacccggatt tccaggtgag catacgcgca gaccctaagt
       61 aacagccatc acgagttttt ggacgtaata gccgtcacgt cgcctcagga aagcacctga
      121 agcgctccag acgcaatcgg agacttgccc ggaaacggtt catacgacta gctttaacgg
      181 ctattgcttt ggggagtcca gcgtgaaggc caatctgcat cccgtctacc ggaagatcac
      241 cgcggtctgt gcgtgcggga atcagttcga aacccgctcg acggcctcgt ctatccatgt
      301 cgaggtctgc gctcagtgcc atccgttctt tacgggcaaa cagcgcctgg tggacaccgc
      361 cggacgggtg gatcggttcc ggcgcaagta taagagcgag acggcgccgg ccaaggcgta
      421 atcgatggag aaccgactcc gccaggccct cgcccgagcg gaggaggtag cccaggcgct
      481 ggctgatccc gatgtgtccc gggatcccgc gcggctccgg tcgctgggtc gggagcatac
      541 gcggttggtc ccggtagtga aggcggccga gcggttggcg cggctcaggg acgaacttca
      601 acaggctcgg gagctggccg gagaggccga tcccgagctt gttgcgttgg cccgtgccga
      661 cctgaatcgg cttggccccg cgatcgagga tctgcaagcc gagctgcatg agcttctcct
      721 tccccgcgat ccccacgatg accgggacgc cattgtcgag gtgcgtgccg gaaccggcgg
      781 ggacgaggcg ggtctctttg cggctgagct ggggcggatg tatacccgct acgccgaacg
      841 gcacgggctc gcggtcgagc cgatttcggt gagcgagggg aacctgggcg gcgtcaagga
      901 agccattttt gcggtgcgcg ggaccggcgc gtatggactt ctccgctggg agtcgggagt
      961 gcaccgggtc cagcgcgtcc ctcaaaccga aacgcagggc cggatacaca cctctgctgc
     1021 aaccgttgcc gtgcttcctg aagcggaaga ggttgatgtc aagatcgagc cgcaggagct
     1081 gaaggtcgac gtgttccgct ccagcggccc gggaggtcag agcgtcaaca ccaccgacag
     1141 cgccgtgcgc atcacccatc ttccaaccgg cctggtggtt cagcagcagg atcagaaatc
     1201 tcagctgcag aacaagatca aggcgatgga agtcctccgg gcgcgactgc tcgaccggat
     1261 gatcgcggag caggaggctg cgcgagcacg agaccggaga gcgatggtgg gtaccggcga
     1321 tcggtcggcc aagattcgga cctacaactt tcctcagagc cgggtcaccg atcaccgcat
     1381 caacctgtcg gtgcacaatc ttgacgacgt gatgaacggg tctctggagc cgttcgtgaa
     1441 tgcgctgcga ctggccagcc gcgaggagca ggtgagtgcc tgagctgacc ggatcacttg
     1501 ccagtctcct ggccgaagcc agactgatcc tgggcgccgc cgggattccg gagcctgcca
     1561 gggaagctct ccttttgtgg gcggatttga gcggtgaggc ggctgcaggt gtcctgctcg
     1621 cgagctcgcg gacggttgat ccggacatgg caaccggatt tctcgccgcc attgaccgca
     1681 ggacaaccgg agagccgctg gcgtatgtta ccggctggac cgggttccgc ctgctcaaac
     1741 ttcgcatcga ccggcgggcg ttgattcctc ggccggagac ggaaggcctg gtcgagttgg
     1801 tgctcgagcg ggtcgatgag gggcgaatcg tagatgtggg gaccggctcg ggctgcgttg
     1861 cgctcagcct ggcgaccgaa ggccgctaca aaagcgtagt cgccatcgac aagtcggcgg
     1921 ccgcgctcag actcgcggcg cttaaccgtg agctggtggg cgcctcggtt gccctggttc
     1981 gggctgatct caccaccacg ctccgcgacg catgcgtgga cgcgctggtc tcgaacccac
     2041 cctacctcac agagcaggag tacgccggtc tggatccggc agtctctctc tgggagcctc
     2101 gcgaggcgct ggtgagcggc cgcggaggca tggatgcaac ggcgcggctg ctcgacgacg
     2161 gacgcagggt agtgcgggcg ggaggctgga tcgctctgga gctggatgcc actcgggcgg
     2221 cgatggtcgc ggagacggcg gagcgccgcg gatgggaggc ggtcgaagtg cgggcggacc
     2281 ttttcgggcg cgagcgttac ctgcttgcgc ggaggagcaa aacaccgtga tctgggacaa
     2341 ggcacaggag cttggccggc tcatcggcca gacgccggag taccaagcgc tgcggcgcgc
     2401 cgagacgaat cttcgcgaag atcaggacac ccaggccaag ctcgaccaga tccagcgact
     2461 cgcccgtcag gtggatcaga tggtggccca gggccaaatg cccgaccagc ccaccgcgga
     2521 agcctatgag accgcggtgc gggatctgga aatgagcggg tcggggcagg cgtacgtcgt
     2581 tgcccgggcc aatttcgaga agctgatgac gaaggtgaat cagcagatca gcgagggaat
     2641 ggaaaagggc gcgaccagca ctatcatcac cctctcgtga acgaggggtt tttcgtcgtc
     2701 gtcgaaggac cggagggggc cggcaagtcc accctcgccg ctggcctagt tcggctgatg
     2761 caggaattgg gatgggatcc ggtcggcgta cgcgagccgg gaggaactcc ggcggcagag
     2821 gcggtcaggg cagccttcct ggatccggcg aaccggttcg ccccccacac ggaactgctg
     2881 tacatttgcg cggcccgggc ccatttggtg caggaggtca tccagccggc cctggctgcc
     2941 gggcgcccgg tcgtctctga tcgctacgag ctctccactt tggcctatca aggggcggga
     3001 cggggtctgg atccggacat tgtggccttc gtgaaccggg tcgcgactgc agggcttcgg
     3061 ccacacctga cgctggtgct cgacattcca gccgaggttg gcctcgatcg attgaaggcc
     3121 ggcgccaaga agcacgatcg gctggaacag gaagatccag ggtttcatcg ccgggtcgca
     3181 gctgcctatc tagcggctat cggcgacggg gtgcgacatt tgaatgcagt agcgccgccg
     3241 gcggctgttc tcgatgccgc ttgggccgag gtggtcgcgg cggcaccggc aacctttcgc
     3301 tcgggtggag actcccgagc ggttcggagg acggacgtat gaagcagcgt tggggcctga
     3361 ttgggttggt ggcggtgatc tcattcctga gtggcggctg gcttctgcag cgcggagtcg
     3421 cggccggcgg caacgtatac caacaggctc gtctcttcga cgacgtcctc tcccacgttc
     3481 acaactacta cgtggactcc ctgggtgaga gcgatctcta cgacaaagcc accaacggca
     3541 tgctagagga gctcaaggat ccctattccg tgctcctcac cggtgacgac tatcgggctc
     3601 tgaccgagca gacgtccggg aactacgccg gcctgggcat ccagatcgac gtacgggacg
     3661 gctggattac cgtcgtggcg ccgcttcccg atacgcctgc cgaacgggcc ggcattcaga
     3721 ccggcgacca gattgtcgag gtggacggga agtccaccga gggctggaag aacgatcagg
     3781 cggtgaaggc gctccggggt gaggccggca gcaagatcgc cgtgaaggtg cgtcgctccg
     3841 gaatcgcaga cccgatcacc ttcgacctga ctcgcgccac catccacatc aggtcggttc
     3901 cgcctggcac gttgttcgac ggcggtatcg gctacatctc cttgaacccg gttgccgaaa
     3961 cgtcggctca ggagctgcgc gacgagatca ccagcatgaa gtccaagggc atgaagtcga
     4021 tgatcctcga tcttcggctg aatcccggtg gcctgctgga tcagggtgtc aaggtcgccg
     4081 agctattcct ggacaagggt caggagatcg tatccaccaa ggggcgtgca cgaggctcga
     4141 gcaaggagtt ctacgacgaa gtcaagcagc tctggcccga tctcccgatt gtcgtgctgg
     4201 tcaatgaagg tacggcgagc gctgcggaga tcatcgccgg ggcgctccag gatcacgacc
     4261 gggcggtcgt ggtcggctcc cccacgttcg gcaagggcct ggttcaaacg ctattccctc
     4321 tgggcgacgg tgtcgccctt aaactgacga cggcccgttg gtacaccccg agcggccgaa
     4381 ccattcaacg gatagccaag gacgaagagg atcagctggc ccaggcggaa tcggctgcgg
     4441 ccggtacgct gaccgcggaa agcaccgact cggctctcaa ggagcggccg atctaccgca
     4501 ctgacgcggg gcgcgcggtg cggggtggtg gtggtatcgt gccggacctc gtgattcgcc
     4561 cggatacgct tacgaccggc gagcgcacgt ttgccaaggc gctcggcgcc aagctcccgg
     4621 tgtaccgcga cgtgctcacg acctacgcgc tcgagttgaa gcggaccaag ggcgtcgaga
     4681 acgagagctt caagatctcg cccgagatgc ggcgggaagt cttccagcgg ttgcggaaca
     4741 agggcgtgga gctcacccag gccgacttca gcgcagccgg tcaactggtg gatgagcaac
     4801 tgggctatga aatcgagcga tacgtgttcg gccgtccggc cgaattccgt cgccgctcgt
     4861 tggacgaccg ccagctgcag tcggcgctcg gtctcctgaa gaaagctcag acgcccaagg
     4921 acctcatggg catcgcgatg
//
LOCUS       DASRJQ010000090         4592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_505751, whole genome shotgun sequence.
ACCESSION   DASRJQ010000090 DASRJQ010000000
VERSION     DASRJQ010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505751"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..1620)
                     /locus_tag="VFB89_02075"
     CDS             complement(157..1620)
                     /locus_tag="VFB89_02075"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HZM26119.1"
                     /translation="MDRRLAILPIALAAACATNPVTGKSELALVSESQEIEMGRQSAQ
                     EVAQTIGYYNDPAIQSYVSAIGLKMAKVSERPQLPWEFHVVNDASVNAFALPGGFIYV
                     TRGLMTSITNEAELATVVGHEIGHVTNRHQVQQISRAEVAQLGLGVGMILRPDLQPLG
                     SALGSGLSILFLKYGRDAENQADLAGFRYALNQNYDVRQMINMFQTLEGVSQLEGGGK
                     LPEWLSTHPNPENRAQATQARLDTLHRDLTNTIVGRDEYVQRLKGMVYGEDPRQGYFQ
                     GTTFLHPDLRFQLSFPQGWKTQNQADAVVGVSQNQDAMMQLSLAGKTDPTSAARQFLS
                     QQGIQAGQGSTQSINGMPAATSYFSAQTEQGQIQGLVSFISYRGTTYGILAYTGAGNL
                     QAYDGTFRRAIGSFRELNDPAALNVQPARLELVKLDQPMTLAEFNQRYPSTVRVEEVA
                     LINGVQSPSATLKAGELYKRVVGGLKPTNSSNLTGQQ"
     gene            complement(1737..2444)
                     /locus_tag="VFB89_02080"
     CDS             complement(1737..2444)
                     /locus_tag="VFB89_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26120.1"
                     /translation="MSEAPGAEPSREEQTARARPEVMFLNRLEVLERDNRRLQRSANL
                     SFIGIAILLGLVVALVWVAGRHGMPGSVAREVAARQFVLRDQSGTLRGVWGMGEDGAV
                     RLVLQDQTGRARVKVSLLEDGTSGLSFSDSTGHARAVFALLPDQTGSVAFADESGKTR
                     SVLGISADGSSNIVFADRAGSTRAGLGVDARGQSTFTLADKMSEALPTPAVTESVGGE
                     VADSAPAHKRPIPRRKP"
     gene            complement(2441..3781)
                     /locus_tag="VFB89_02085"
     CDS             complement(2441..3781)
                     /locus_tag="VFB89_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26121.1"
                     /translation="MSAAPIPTPPRPIAPTRPPLPESIEATGLSIEFVVDLLLKTLYV
                     QGARTGQQLMETVKLPFPFVDDMLLGLQQRRLTEVRGTSGAGRGGYMFDLTSEGRTRA
                     REALESSQYIGPAPVPLAQYSEWTMRQSISRVHVSRDSVRLGFRNMVLAEPILEMLGP
                     AINSAKSLFLYGGPGNGKTLIAESISRMLGGHLYIPYAIEVDGQILVLYDPVHHHQVP
                     DDEPLLTADQPAWLKQIPEYDRRYVPVRRPVVLTGGELTLDQLDLQYDSYAKMYQAPF
                     QLKANGGVLILDDFGRQRTPPRDLLNRWIVPLEKRIDFLTLHTGGKFPVPFDCLLIFS
                     TNLAPHELVEEAFLRRIHYKLHVFGPTQQEYAEIFRRCCAEREITYESWVVPHMYAEY
                     YGRRQIVPRNCHPRDVLDHFLDMVHYLEVAPKLTPDLLDRACNSYFLDAPQEVT"
     gene            complement(3778..4146)
                     /locus_tag="VFB89_02090"
     CDS             complement(3778..4146)
                     /locus_tag="VFB89_02090"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HZM26122.1"
                     /translation="MSVRFLRAANQRGRVLGDRIVVADRWWLRLKGLMGRSSLAPGEG
                     LLLRPCRAVHMMGMRFPLDIAFLDTNGTVVAAYADLRPGGRTGWHRQADGALELPAGT
                     LAASGTREGDQIVCTEEFHS"
     gene            complement(4143..>4592)
                     /locus_tag="VFB89_02095"
     CDS             complement(4143..>4592)
                     /locus_tag="VFB89_02095"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZM26123.1"
                     /translation="QKALPDALDLLVVCVEAGLGLNQALVRVSEEIERLSPVLSEQFV
                     LVNLEIRAGTPRDDAFRNMGERTGLDDLKSLMAMLIQTDRFGTSIAQALRVQADTLRT
                     KRRQRAEEAAAKTTIKLVFPLVLFIFPAMFVVILGSAIIQIFEALGH"
BASE COUNT          827 a         1450 c         1490 g          825 t
ORIGIN      
        1 atgcgatctt tgcgagggcg agaaggatac tggaaaaagc gtgaacggta aacagtgaac
       61 cgggggctcc ttgtgtcgcg gggttggggg tgctggggtt ggcggggcat ggccagcgcc
      121 cccaaccccg cgacacaagg agcccccgtc tatcgtttac tgttgaccgg tcaaattgct
      181 gctgttggtc ggctttaggc cgccgacaac tcgcttgtac aactctcctg ccttgagggt
      241 tgcgctgggg ctctggaccc cgttgatcag ggccacttcc tcgactctca ccgtggacgg
      301 ataccgctgg ttgaactccg ccaatgtcat tggctggtcc agcttcacca gctcgagccg
      361 cgcgggctga acgttgagag cggccggatc gttcagctcg cggaagctgc cgatcgcacg
      421 acgaaaggtt ccgtcgtacg cctgcagatt tccggccccg gtgtacgcaa gaatgccgta
      481 ggttgtaccc cggtacgaga tgaacgagac cagtccctgg atctgcccct gctcggtctg
      541 cgcgctgaag tagctcgtgg ccgcaggcat tccgttgatt gattgcgtgc tgccctgccc
      601 cgcctgaatt ccctgctgcg acaggaactg ccgtgcggcc gaggtgggat cggtcttgcc
      661 ggccagcgag agctgcatca tcgcatcctg gttctggctc acgcccacca ctgcatccgc
      721 ctgattctgg gtcttccacc cctgggggaa ggagagctgg aatctcagat cgggatggag
      781 gaaggtcgtc ccctggaagt aaccctgcct ggggtcctcg ccgtacacca tgcccttgag
      841 ccgctggacg tactcgtcgc gcccgacaat cgtgttggtc agatcccggt gaagcgtgtc
      901 gaggcgggcc tgcgtggcct gcgcgcggtt ctcgggattc gggtgggtag agagccactc
      961 gggcagctta ccgcctccct ccagctggct cacgccttcg agcgtctgga acatgttgat
     1021 catctgccgg acgtcgtagt tctggttcag cgcatagcga aatccagcga ggtccgcctg
     1081 gttctcggcg tcgcggccgt acttgaggaa caggatactg agcccgctcc cgagggccga
     1141 accgaggggc tggaggtcgg gtcttagaat catgccgacc ccgagaccca gctgggcgac
     1201 ctcggcacgc gagatctgtt gcacctggtg gcggttggtg acgtgcccga tctcgtgtcc
     1261 caccacggtg gccagctcag cttcgttggt gatgctggtc atcagaccac gggtcacgta
     1321 gatgaatcct cccggcagcg cgaacgcatt gaccgaggcg tcgttcacga catgaaattc
     1381 ccacggcagc tgaggacgct ccgagacctt ggccatcttg aggccgatgg cggagacata
     1441 gctctggatc gcagggtcat tgtaataccc gatggtctgg gcgacctcct gggcgctctg
     1501 cctgcccatc tcgatctcct gactttcgga gacaagagcc agctccgact tgccggtgac
     1561 cgggttcgtc gcgcaggcgg cggcgagagc gatcggaaga atcgcaaggc gccgatccat
     1621 ggggaccctc ctcggggtgt tggaagtgca aagctaaaca cgagagagcc ggcggaccgt
     1681 gaggtccgtc ggctccccgt accctccgat gggcggcgtc tgccttaggt gataggctag
     1741 ggtttccgcc gagggatagg acgcttgtga gcgggagcag aatcggccac ctcaccgccc
     1801 acgctttccg tgacggcggg cgttggcagc gcttcactca tcttatcggc cagcgtaaag
     1861 gtgctctgtc cccgtgcgtc cacacccagc cctgcccggg tcgagcccgc acgatcggcg
     1921 aagacgatat tggaggagcc gtcggcggag atgcccagca cgctgcgggt cttcccgctc
     1981 tcgtcggcaa aggcgacgga gccggtctgg tcgggaagga gcgcgaacac ggcgcgagca
     2041 tggccggtgg aatcggagaa ggaaagcccg gaggtaccgt cctcgaggag gctgaccttg
     2101 acccgcgccc ggcctgtctg gtcctgaaga accagccgga ctgcgccatc ctctcccatg
     2161 ccccagactc cgcgcagagt tccgctctga tcccggagaa cgaactgacg tgcggcgact
     2221 tccctggcca cgctaccggg catcccgtga cgcccggcaa cccacacgag tgcgacgacc
     2281 aggcccagca gaatcgcgat gccgatgaac gagaggttgg ccgagcgctg gagcctgcga
     2341 ttgtcccgct ccagaacttc caaccgattc aggaacatga cctcggggcg cgcccgggca
     2401 gtctgttcct cgcgtgaagg ctcggcgccc ggggcttcgc tcacgtgacc tcctgcggag
     2461 catcgaggaa gtaggagttg cacgcccgat ccagcaggtc gggagtgagc ttgggagcca
     2521 cctcgaggta gtgaaccatg tcgaggaaat ggtccagcac atcgcgggga tgacagttcc
     2581 ggggaacgat ctgccgccgg ccatagtact cggcgtacat atgcgggact acccaggact
     2641 cgtaggtaat ctctcgctcg gcacaacagc ggcggaagat ctcggcgtac tcctgctggg
     2701 tgggcccgaa gacgtgaagc ttgtaatgga ttcggcgcag gaacgcttct tcgaccagct
     2761 cgtgcggcgc gaggttggtc gagaaaatca gcaggcagtc aaacggcacc ggaaacttgc
     2821 caccggtgtg cagcgtgagg aaatcgatgc gcttctccaa cggcacgatc cagcggttga
     2881 gcaggtcccg aggaggagtc cgctgccgtc cgaagtcgtc gagaatgagc acgccgccgt
     2941 tggccttcaa ctgaaacggc gcctggtaca tcttggcgta gctgtcgtac tggagatcaa
     3001 gctggtccag ggtgagctcg ccacccgtca gcacgacggg ccggcggaca gggacgtaac
     3061 gccggtcgta ctccggtatc tgcttgagcc aggccggctg gtccgccgtg agcagcggct
     3121 cgtcatccgg aacctggtga tggtggaccg ggtcgtagag caccaggatc tggccgtcca
     3181 cctcgatggc gtaggggatg taaaggtggc cgcctagcat ccgagagatg ctttccgcga
     3241 tgagcgtctt gccgttgccc ggcccgccgt acaggaacag cgatttggcg gagttgatgg
     3301 ctggccccag catctcgagg atgggctcgg cgagcaccat gttgcggaac ccgagcctga
     3361 cgctatccct ggacacatgg acccgcgaga tcgactgccg catggtccac tcgctgtact
     3421 gcgccagcgg taccggggcg ggcccgatgt actggctcga ctccagcgct tcgcgagccc
     3481 gggtgcggcc ctctgaggtg agatcgaaca tgtagccgcc ccgccctgca ccgctggttc
     3541 cgcggacttc cgtcaggcga cgctgctgca gtcccaggag catgtcgtct acgaatggaa
     3601 acggaagctt cacggtttcc atcagttgct gcccggtacg ggcaccttgc acgtacagcg
     3661 tcttgagcag cagatccacc acgaactcga tggacagtcc cgttgcctcg atcgactcgg
     3721 gtaagggagg tcgggtcggt gcgatgggcc gtggtggagt cgggatggga gcggcgctca
     3781 tgaatgaaac tcctccgtgc acacgatctg atccccttcg cgggtcccgc tcgccgccag
     3841 cgttccggcc ggcagctcga gggctccgtc cgcctgccga tgccaaccgg ttcggccacc
     3901 gggccgcagg tcggcatacg ctgccaccac ggtgccgttg gtatcgagga atgcgatgtc
     3961 gagcggaaac cgcatcccca tcatgtggac cgcccggcag ggccgaagca acaagccttc
     4021 acctggcgcg aggctggagc gacccatcaa gcctttcagg cgcaaccacc aacggtcggc
     4081 gaccacgatg cgatcgccca gcactcggcc cctctggttg gcagctcgca gaaaccgaac
     4141 gctcaatggc ccagcgcttc gaagatctgg atgatcgccg agcccaggat cacaacgaac
     4201 atcgcgggga agatgaacag caccagcggg aagaccagct tgatcgtcgt cttggccgcg
     4261 gcctcctcgg cccgctggcg gcgcttggtt cgcagcgtgt ctgcctgcac tctgagcgcc
     4321 tgcgcgatcg aggtaccgaa tcgatcggtc tgaatgagca tcgccatcag cgacttgaga
     4381 tcatccagac cggtgcgctc gcccatgttg cggaaggcat cgtcccgcgg ggtgccggcc
     4441 cgaatctcca ggttgaccag aacaaactgt tcgctcaaca ccgggctcaa ccgctcgatc
     4501 tcctcggaca cgcggaccag cgcctggttg aggcccagcc cggcctcgac gcacaccacc
     4561 agcaggtcca acgcgtcagg caacgccttc tg
//
LOCUS       DASRJQ010000091         3543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2787760, whole genome shotgun sequence.
ACCESSION   DASRJQ010000091 DASRJQ010000000
VERSION     DASRJQ010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2787760"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2073)
                     /locus_tag="VFB89_02100"
     CDS             complement(<1..2073)
                     /locus_tag="VFB89_02100"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HZM26124.1"
                     /translation="MTAVATERVLARLARPLRLRSRAAWAAAAVGTAALVLGSAAWLA
                     RWEILTAPWWVLAAWVVAAVALAVVAVIAWRQETSLSVRAFARCLERDGGWRHGAISA
                     LLDRPAPGTSEALFEMADAAGAADLANRGSTAAAPLTRSVRRRLLSGLAALAVGLSIL
                     GAAGPMRGVAAALWHPARAWEATLAPVRLRAGATQVDRGAKVALDVEAIGRRHVTLWL
                     RAPGEAWRPLLLPLDSVGRGRFLAGPLESDLFARATSGRRSSDTIEVRIRLPVFLGAL
                     TVTAHYPSYLKLDDEPVQLGGDTILIPAGTRLETKGEATAPLASAGWKSAGTSEDLSV
                     SAARFSGNFTPRSSGVYRLSLVTAAGAPLAGDSVRLPIRLVADSAPRVDVPIPGADTI
                     APLDLQIPLVIDARDDHGIRAVTLESRRISQLGGADPVRREAVPLPAGTTDRGILSYT
                     LDINRRGLLPGDTLRYVAVATDNSPAGQTGRSREFVLRLPTMSEVRAAERQASRTVAG
                     RLDSLAAESRRLERRTEDLAREQPRSDRRSGDRPEALPYEEAKRAEAVAAQQENLVAQ
                     AEALRQSLESLEKAAEAAGLKDSAWQQQLAEIRNQLERALTPELREQLQKLQQALKDL
                     NADATKEALEDLTERQRQLREALEQAKELFRRAALEGDLANLSQESRDLSEQQKRWNE
                     QVNATDSAR"
     gene            complement(2070..2777)
                     /locus_tag="VFB89_02105"
     CDS             complement(2070..2777)
                     /locus_tag="VFB89_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HZM26125.1"
                     /translation="MTSRSGGLALLVALATATPGARLRSESRLLGRSEPPAVMTIGRL
                     HYDGGGDWYANPSSLPNLLQAIRTRTTLRVGTQEKVVTLGDEDLWTIPYLHMTGHGNV
                     HFSNQDLATLREWLRQGGFLHASDNYGMDASIRRELARLFPGHPLVEVPLNHPIYHLV
                     YDFPKGLPKIHEHDGKPAQGFGIFLDGRLVVFYDYQCDLGDGWEDPDVHHDPPEKHEA
                     ALRMGVNLFAYAVGYGG"
     gene            complement(2794..>3543)
                     /gene="rsmI"
                     /locus_tag="VFB89_02110"
     CDS             complement(2794..>3543)
                     /gene="rsmI"
                     /locus_tag="VFB89_02110"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HZM26126.1"
                     /translation="RVAVVAAEDTRRTRGLLAYLDARPQLLSFHAHSGPRRTEVLLEI
                     LSEGRDVALVTDAGTPGVSDPGPGLVEAARKAGFPVVPIPGASAVTAGLSASGLPADR
                     YLMLGFVPRKGAERVRLLTRAVEEEWPAVFFEAPGRLAALLRDLARIAGPDRRAVVAR
                     ELTKLHEEFRDGTLTELADYYSREEPKGEITLILAGTGVPPAPADRSQEALALARRLL
                     EAGVSRREAAHRVAAELGLPRNEAYRLVMEL"
BASE COUNT          587 a         1226 c         1171 g          559 t
ORIGIN      
        1 ccgtgcactg tccgtggcgt tcacctgttc attccagcgc ttttgctgct cgctgagatc
       61 acgcgactcc tgggacaggt tggccagatc accttccaga gccgcccggc gaaacagctc
      121 cttggcttgc tcaagggcct cgcgaagctg ccgctgtcgc tcagtgagat cctccagagc
      181 ttccttggtc gcgtccgcgt tgagatcctt gagtgcctgc tgaagcttct gcagctgctc
      241 ccgcagctcc ggggtgagtg cccgctccag ctgattgcga atctccgcga gctgctgctg
      301 ccacgcagag tccttgaggc cggccgcttc agcagccttc tcgagcgact ccagcgactg
      361 ccgcagggcc tcggcctggg ccaccaaatt ttcctgctga gcagccactg cctccgctcg
      421 cttggcttcc tcgtacggca gcgcctccgg acgatccccg ctccgtcgat cgcttcgggg
      481 ttgctcccgg gctaggtcct cggtccggcg ctcgagcctg cgactctccg cggcgaggct
      541 gtccagccga ccagcaacgg tccggctggc ctgccgctcg gcagcccgca cctcgctcat
      601 tgtcggtagc cggagcacga actcgcgcga gcgtccggtc tgcccggccg gcgagttgtc
      661 cgtcgccact gcgacgtagc gcagtgtgtc tcccggcagg agcccgcgac ggttgatgtc
      721 aagcgtgtag gacaggattc cgcgatccgt cgtgccggca gggagtggga ccgcctcgcg
      781 tcggaccggg tcggcacccc cgagctgact gatacggcgg ctctcgagcg ttactgcgcg
      841 gatcccgtga tcgtcgcggg cgtcgatgac aagagggatc tggagatcga gcggggcgat
      901 cgtatcggcg ccgggaatcg gaacgtccac ccgcggcgca ctgtcggcca ccagacgaat
      961 cggcagccgg acgctgtcgc ctgccagcgg agcgccggca gcggtgacca gggagagccg
     1021 gtaaacgcct gagcttcggg gcgtgaagtt gccggagaat cgagccgccg aaaccgaaag
     1081 gtcctcgctc gtcccggcgc tcttccagcc tgcggaagcc agcggggcgg tcgcctctcc
     1141 cttggtctcg aggcgagtgc ctgccggaat caggatggtg tcgccgccca actgcacggg
     1201 ctcatcgtcg agcttgagat aactcggata gtgcgcggtc acggtaaggg cgccgagaaa
     1261 gacgggcaac cgaatgcgga cttcgatcgt gtccgagctt cggcgtccgc tggtggctct
     1321 ggcgaagagg tcgctttcga gcgggcccgc gagaaagcgg ccacgcccta ccgaatcgag
     1381 tgggagaagc aatggtcgcc aggcttcgcc cggagcgcgt agccagagag ttacgtgccg
     1441 ccgcccgata gcttcgacat cgagggctac tttcgctccc cgatctacct gtgtagcccc
     1501 agcacgaagc cgaaccggag cgagcgtcgc ctcccaagcc cgggcgggat gccagagagc
     1561 cgccgccacc ccccgcatcg ggccggcagc tccaagaatg gacagcccta cagccaatgc
     1621 cgcgaggccg ctgagcaagc gccggcgcac cgagcgagta agcggagccg ctgcggttga
     1681 ccccctattg gccaggtcgg ccgcgccggc ggcatcggcc atttcaaata gcgcttcgct
     1741 ggttccggga gcgggacggt cgagcagcgc ggagatggcc ccatgccgcc aaccgccgtc
     1801 ccgctccagg catcgggcaa acgcacggac ggacagcgag gtctcttgtc gccacgcgat
     1861 gacggcgacg acggccaacg ccacagccgc gaccacccat gcagcgagga cccaccaggg
     1921 agccgtgaga atctcccagc gcgcgagcca ggcggccgag cccagcacca gcgccgccgt
     1981 gccgactgcg gctgctgccc aagcggctcg actgcgcagc cgaagcggac gcgccaatcg
     2041 cgcgagcacc cgctcggttg cgactgccgt catcctccgt atcccaccgc gtacgcgaag
     2101 agattcacgc ccatccggag tgccgcctca tgtttctcgg ggggatcgtg atgcacgtcg
     2161 ggatcttccc agccgtcgcc caggtcacac tgatagtcgt agaacacgac gagtcgaccg
     2221 tcgagaaaga tcccgaaccc ctgggccggc ttgccatcgt gctcgtggat cttgggcagg
     2281 cctttgggaa agtcgtaaac aagatgatag atcgggtgat tcagcgggac ctcgaccagt
     2341 gggtgacccg ggaagagccg ggccagctcc cggcggatcg aggcatccat tccgtagttg
     2401 tcactggcgt gaaggaagcc gccctgccgc agccactccc ggagcgtcgc caggtcctga
     2461 ttcgagaaat ggacgttccc gtggcctgtc atatgaaggt aaggaatggt ccacagatct
     2521 tcgtcgccga gggtgacgac cttctcctgg gttccgacgc gcagggtggt acgggtgcga
     2581 atggcctgaa gcaggttggg aagactggag gggttggcgt accagtcgcc gcccccgtcg
     2641 taatggaggc gaccgatcgt catcaccgcc ggcggctccg aacgccccag cagccgggac
     2701 tccgaacgga gtcgtgcccc gggggtggcg gtcgcgaggg ccaccagcag ggccaacccg
     2761 cccgaacgac tcgtcaccgg aatgcccgcc gcgtcatagc tccatcacca gacgatacgc
     2821 ctcgttgcgc ggaagcccca gctcggccgc gacccggtga gccgcttcgc gccggctgac
     2881 gccagcttcg agcagccgac gggccaacgc cagggcttct tggctgcgat ctgccggtgc
     2941 cggcggtacg ccggtgcccg ccagaatcag cgtgatctca cctttgggct cctctcgcga
     3001 ataataatcc gcgagctccg tcagggttcc gtctctgaac tcttcgtgca gcttggtaag
     3061 ctcccgcgcc acgactgcgc gccggtccgg ccccgcgata cgcgccaggt ctcggagcaa
     3121 cgcggccagg cggccgggtg cctcgaaaaa aacggccggc cactcctctt ccacggcgcg
     3181 ggtaagcagg cgcacgcgct ccgcgccttt tctggggacg aatccgagca tcaggtatcg
     3241 gtcagctggg agtcccgacg ccgacagccc tgccgtaacg gccgacgccc ccggaatcgg
     3301 gacgaccgga aagcccgcct tgcgcgccgc ctccaccagg ccagggccgg gatcgctcac
     3361 gccgggcgtt cccgcgtctg tcaccagcgc cacgtcgcgt ccctccgaca ggatctcgag
     3421 caggacctcc gtacgccggg ggccggagtg cgcgtgaaag ctgaggagtt ggggtcgtgc
     3481 atcgaggtag gcgagcagac cgcgagtgcg ccgcgtgtct tctgccgcga ccaccgcgac
     3541 ccg
//
LOCUS       DASRJQ010000092         3129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3079983, whole genome shotgun sequence.
ACCESSION   DASRJQ010000092 DASRJQ010000000
VERSION     DASRJQ010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3079983"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VFB89_02115"
     CDS             complement(<1..755)
                     /locus_tag="VFB89_02115"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZM26127.1"
                     /translation="MTTPTTAEAGHASNEATVVKIDGFRGRLISPDDADYDIARAVWN
                     GAIDRSPRLIARCIGPADVVAAVRFARDHDLEIAVRGGGHNVAGTAVCDDGIVIDLSA
                     MRAVRVDPADRRAWVQGGALWGDVDHETQAHGLATSGGIVSHTGVAGLTLGGGVGWLM
                     RKHGLTVDNLLAVNVVTADGEPLRVSEDEHPDLFWALRGGGGNFGVVTSFDFRLHPVG
                     PTILAGPILWDATDTGEVLRFYRDFVRDAPDELG"
     gene            842..1558
                     /locus_tag="VFB89_02120"
     CDS             842..1558
                     /locus_tag="VFB89_02120"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZM26128.1"
                     /translation="MRNTGVVRTYGQYCPIARGAEIFAERWTPLIIRNLHLGCATFSE
                     ILEGAPGLSRTLLSQRLRQLERFGVVESARKPDGRGHHYELTSAGHDLFTVCQSLGEW
                     GARWLEIAPENLDPFVALWSMCNALRRDRLPDRRIVIRFDFTGRVQRERYWLLIEHGD
                     TEICKTSPGLDDDLYITAEAEAFVKWHAGQLTWAQATRESRIQLDGPPSLVRAFPTWN
                     ARSMFAHIRPVSRTATAAPG"
     gene            complement(1704..1913)
                     /locus_tag="VFB89_02125"
     CDS             complement(1704..1913)
                     /locus_tag="VFB89_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26129.1"
                     /translation="MRAYLQITGALFGLIAVVHVLRLIRHWPIQLAEYSVPLWASWVG
                     LIVAGALSIWAVRLLRATDRAVPSN"
     gene            complement(2613..3005)
                     /locus_tag="VFB89_02130"
     CDS             complement(2613..3005)
                     /locus_tag="VFB89_02130"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HZM26130.1"
                     /translation="MFFYGLFMDPELLQDRGILPTDIRVASVSGFALRIGARATLVPN
                     LGGQVHGVLMKLSHADLERLYADPSVRAYRPEAVLAVTRDGVTVAAMCYNLAEVPSPR
                     ERNAEYASKLRSLAQAIGLPESYVASIS"
BASE COUNT          554 a         1076 c          992 g          507 t
ORIGIN      
        1 ccgagctcgt cgggagcgtc gcggacgaaa tcgcggtaga agcggaggac ctcccctgtg
       61 tcggtcgcgt cccagaggat gggcccggcc aggatggtgg gcccgacggg atggaggcgg
      121 aaatcgaacg aggtgaccac cccgaagttg ccgccgccgc ctcgcagtgc ccagaacagg
      181 tcggggtgct cgtcctcgga cacgcgcagc ggctcgccgt ccgccgtcac cacgttgacg
      241 gcgaggaggt tgtcgaccgt gaggccgtgc ttgcgcatca accagccgac gccaccgccg
      301 agggtgagcc cggcgacgcc ggtgtggctc acgattcccc cgctggtggc cagaccgtga
      361 gcctgcgtct cgtggtcgac gtcgccccac agtgcaccgc cctgcaccca ggccctgcgg
      421 tcggcgggat cgacccgcac ggcccgcatc gccgagaggt cgatgacgat cccgtcgtcg
      481 cagacggcgg tgcccgccac gttatggcct ccgccccgta cggcgatttc cagatcgtgg
      541 tcgcgggcga aacgcacggc cgcgaccacg tcggctggcc cgatgcaccg ggcgatcagg
      601 cgcggggacc ggtcgattgc gccgttccac accgcacggg cgatgtcgta gtcagcgtcg
      661 tcgggactga tcaaccggcc tcggaagccg tcgatcttca cgacggtcgc ctcgttgctc
      721 gcgtggccgg cttccgcggt cgtcggtgtc gtcatcgggt cctccgctct cgacggtcac
      781 atcgtacgac cggggaccgt ggccgtaaag tcaagaatct ggacttgacg accgaaggca
      841 ggtgcgcaac actggcgtcg tgaggacgta cggccagtac tgccctatcg cccgtggcgc
      901 cgagatcttc gccgagcgct ggacaccgct gatcatccgc aacctgcacc ttggctgcgc
      961 aaccttcagt gagatcctgg agggtgcgcc cggactctcc cgtaccctgc tctcacagcg
     1021 gctcaggcag ctcgagcggt tcggggtggt agagtcggca cgcaagcccg atgggcgcgg
     1081 gcaccactac gagctcacgt ccgcgggcca cgacctgttc acggtctgcc agtcgctcgg
     1141 cgagtggggt gcacgttggc tcgagatcgc tcccgagaac ctcgacccct ttgtggccct
     1201 gtggtcgatg tgcaacgcgc tccgccgaga ccggctcccg gatcgacgca tcgtcatccg
     1261 cttcgacttc acgggccgtg tgcagcgtga gcgctattgg ctgctcatcg agcatggaga
     1321 caccgagatc tgcaagacgt cccccggcct cgacgacgac ctctatatca cggcggaagc
     1381 cgaggccttc gtcaaatggc acgccggcca gctcacgtgg gcccaagcca cccgcgagag
     1441 ccgcatccag ctcgacggcc ccccgtcgct cgtcagagct ttcccgacct ggaacgcccg
     1501 cagcatgttc gcccatatcc ggccggtctc ccgcaccgcc acggccgccc caggctgaca
     1561 tccgcagctt cctccaggca cgctgcgccc gggcggcagt accgctggcg ctactaaggc
     1621 ttgcacttat gtacttgcgg ccagcggagc cgccggcgga cgcgcccgtc agctgcaagc
     1681 gtaggttacg cggcaggcaa ggctcagttg cttggaacgg cgcgatccgt agctcggagc
     1741 aggcgcacgg cccagatgct cagtgccccg gcgacaatca gcccaaccca cgatgcccat
     1801 aggggcaccg aatactccgc cagttgaatc ggccagtgcc ggatcaatcg gagcacatgg
     1861 acaacggcaa taagcccaaa caacgcgccg gtgatttgta gatacgctcg catggtgatc
     1921 ctcctgtagc tccctccggg agccgtggcc gtctaacgac ctacgccgca gctggcgatc
     1981 cgcgcgccct tttgaattct acgttgcgct atccgccaac ggcggaccgc agcgaggccc
     2041 tgaggccact gaggagacat ccgagccgcc ggggaccgcc cggcgcctgg gatccgtagc
     2101 caacccgcgc cggccctgtc cggtgaccgt atgaaacccc gcatccttct aatagggggc
     2161 cgctcaatgg cccgatggct ttcggcaggt gcggagagga gtcaccgatg ctcacagcgc
     2221 tcaaggctta ccagctgcag ttaacctcga gcaggcgatc ctgtttgcgc agctggagat
     2281 tcgtcaccgt ctcgaggccc agcgaatacg agcctaggga ggcgtataga ctggtgcctg
     2341 ggtgcgccag cccagggctc gatcaggtcc gcggcgcatt tagtcatctg ccacccgcca
     2401 acctaccgcc gtctgcggca gcgctcgaga tgctgaggca gagtcgtaca aacaatgcgc
     2461 cgcccgcaga aaggacaggt ctcgcagcac gggacgggat gaagcgccaa tgtcagcggg
     2521 ttgtggcatg gacaatggca ctcctcggac tcgacttggc gaagctctgt actcaccggg
     2581 gccctcccag cgccgccgtt aatgccgctg ctctaagaga tagaggcgac gtaactctcc
     2641 ggcaggccga ttgcctgtgc cagcgaccga agcttggagg catactccgc gttgcgttcc
     2701 cgtggtgacg ggacctccgc aagattatag cacatcgctg ccaccgtgac cccgtcgcgc
     2761 gtaacagcga gtaccgcctc ggggcgatac gctcgtacgc tgggatcagc gtacaacctt
     2821 tcaagatcgg catgcgagag cttcatcaat acgccgtgca cttgcccgcc gagattggga
     2881 acaagcgtcg cccgcgcgcc gatccgcagg gcgaacccgg acaccgaggc gacccggata
     2941 tcggtcggga ggatcccccg gtcctgaagc aactccggat ccatgaatag cccgtaaaag
     3001 aacacgtcga ggcgcctgtg gtccgtgata tcctccgagc gttgtgcagc ggccttacga
     3061 cctatgcttc agttgcgggc ggcccgcgcc tgcaaaacta cgtgcgaccc accatccaag
     3121 gctcgtaag
//
LOCUS       DASRJQ010000093         3242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3368131, whole genome shotgun sequence.
ACCESSION   DASRJQ010000093 DASRJQ010000000
VERSION     DASRJQ010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3368131"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="VFB89_02135"
     CDS             complement(<1..857)
                     /locus_tag="VFB89_02135"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HZM26131.1"
                     /translation="MPWSPEAEQAVLGAMLLDQDAALRAVELVDDTMFYREEHRRLFR
                     AMRALVERRSVIDPVTLNDELERRSELGQIGGPEYVAELVDAAPTAANLEHHTRILRD
                     KAILRRLIEASTQTITEAYDGRSQAPELLDAAEARIFHISQQRLDQGFTRLKEMLWPA
                     MERIEALQRSGKTVTGVPSGFVDLDELTSGFQPSELVVVAARPSMGKTALVLNVAAHA
                     ATATPSTGVAIFSLEMSKDAVVQRLLTAEALVDSSRVRRSALRDADFTRLARAAGILQ
                     GCPMWIDDTP"
     gene            complement(877..1686)
                     /locus_tag="VFB89_02140"
     CDS             complement(877..1686)
                     /locus_tag="VFB89_02140"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HZM26132.1"
                     /translation="MSDERRRYLAQQVELGGAEVILETALVRREAPAGAPASSPPSPS
                     SPTSKSSPPSQQKWKKGAPPIPGPGIYIEPPAPTLLSGNALSELETLDAVAERIRTTH
                     CCGLCPGRTNAVPGEGNPSAQLMFIGEGPGATEDALGRPFVGQAGKLLDEIIQAIGHR
                     REDVYITNIVKCRPPQNRKPLPDEIAACMPYLHRQIALIRPKVIVALGGTAGEALLDA
                     RKSLGELRGKVHSYGGIPLVVTYHPAALLRNPNWKKPTWDDVRIARQLIDR"
     gene            complement(1679..2872)
                     /gene="coaBC"
                     /locus_tag="VFB89_02145"
     CDS             complement(1679..2872)
                     /gene="coaBC"
                     /locus_tag="VFB89_02145"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HZM26133.1"
                     /translation="MWAGRHVVLGVSGGIACYKSCFLARRLVEAGAMVDAVLTQSAAE
                     FVRPVTFEALTGRPVLTSLWDRGGALAHIDLGRVADLIIVAPATANLIARAALGLADD
                     LLTSLLLARRARALLAPAMNDEMYANPITQRNLAILQERGFSLVGPEIGALAEGPSER
                     PGRMSEPETILAHAARLLRGKAALSGRRVVVTAGPTRESIDPVRVVTNRSSGKMGYRV
                     AEAAWERGADVVLISGPVALPSPVGVTVRRVETTKELEDAVRAHLPEADVLVMAAAPA
                     DYRPAHPSASKQPRMQGALAVPMEPTDDILSTTEERRKPGSVIVGFALETGDALDKAR
                     AKLERKHLDLIVVNDALEPGAGFEHDTNRVALLDREGNVRILPLKSKREVAEAILDAV
                     EGRLV"
     gene            complement(2876..3088)
                     /locus_tag="VFB89_02150"
     CDS             complement(2876..3088)
                     /locus_tag="VFB89_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26134.1"
                     /translation="MRITTPHDVAKQAGNKYLGVLVAAKFARYLNELPRDRIVTQGEK
                     LTTKSLDSLVKGDLNYKLVRRRRSEA"
BASE COUNT          529 a         1089 c         1059 g          565 t
ORIGIN      
        1 ggggtgtcgt cgatccacat ggggcagccc tgcaggatgc cggctgcccg ggccaggcga
       61 gtgaaatcgg catcgcgcag ggccgagcga cgcacccgcg agctgtctac cagcgcctcg
      121 gcggtgagca ggcgctgcac gacagcatcc ttggacattt ccagcgagaa aatggcgact
      181 cccgtggacg gcgtggcagt cgccgcgtgt gcggcgacgt tcagcacaag tgccgtcttg
      241 cccatggagg gccgcgccgc gacgaccacc agctcgctgg gctggaagcc tgaggtcagc
      301 tcgtccaagt ctacgaatcc gctgggtacc cccgtaaccg ttttgccgct tcgctggagc
      361 gcttcaatgc gctccatggc gggccacagc atctccttga gtctggtaaa tccctggtcg
      421 agccgctgct gagaaatgtg aaagatccgg gcctcggcgg catcgaggag ctcgggcgcc
      481 tgcgagcggc cgtcgtacgc ctccgtaatc gtctgggtgg atgcttcgat caaccgccgg
      541 aggatggcct tgtcgcgcag aatccgagtg tggtgctcga ggttggccgc tgttggcgcc
      601 gcgtccacca gctcagccac gtactccggg ccgcctatct gtccgagctc gctccggcgc
      661 tcgagctcgt cattgagcgt gaccggatcg atgacgctcc ggcgctccac cagcgcgcgc
      721 atcgcgcgga aaaggcggcg gtgctcctcc cggtagaaca tggtgtcgtc caccagctcc
      781 accgctcgaa gcgcggcatc ctgatcgagc agcattgccc ccaggacggc ctgctccgcc
      841 tcggggctcc atggcaccga tcgaccgacg tacggatcag cggtcgataa gctgtcgtgc
      901 gatacggacg tcatcccagg tgggcttctt ccagttcggg ttacgcagta gcgcggccgg
      961 atggtaggtc acgacgagcg gaatcccgcc gtacgaatgc accttcccgc gcagctcgcc
     1021 caggctcttg cgagcgtcga gcagcgcctc gcccgcggtg ccgccgagcg ccacgatcac
     1081 cttcggccgg atgagcgcga tctggcggtg caagtagggc atgcacgccg cgatctcatc
     1141 gggcagcggt ttccggttct gcggcggacg gcactttacg atattcgtga tgtatacatc
     1201 ctcgcgcctg tgtccgatag cctggatgat ttcgtccaga agcttcccgg cctgacccac
     1261 gaaaggacga cccagcgcat cttcggtcgc tcccggtcct tctccgatga acatgagctg
     1321 ggcagatgga ttgccttcgc ctggaacggc attggtccgc ccgggacaca agccgcagca
     1381 gtgggtggtg cggatccgtt cggcaacggc atcgagggtc tcgagctcgc tcagggcatt
     1441 tccactcagc aacgtggggg ccggcggctc gatgtagatg cccgggccgg gaatcggagg
     1501 agcgcctttc ttccatttct gctgggaagg tggcgaagac ttggaagtgg gagaagaggg
     1561 agaaggggga gaagaggcag gtgcgccggc gggagcctcc ctcctcacca acgccgtctc
     1621 caggatcacc tccgcgccac ccagctccac ctgctgggcc agataacggc gacgctcgtc
     1681 agacaagacg cccctccacc gcatccagaa ttgcctcagc cacttcgcgc ttcgacttga
     1741 ggggcagaat tcgcacattg ccctcgcgat cgagcagtgc aacccgattg gtgtcgtgct
     1801 cgaacccggc gccaggctcg agcgcatcgt tgaccacgat gaggtcgaga tgcttccgct
     1861 ccagcttggc tcgcgccttg tccaacgcgt cgccggtctc gagcgcaaat ccgacgatca
     1921 cgctgcccgg cttgcggcgc tcttcggtgg tgctgagaat gtcgtcggtc ggctccatcg
     1981 gcaccgccag cgcaccctgc atccgaggct gcttgctagc gctgggatgg gcaggccggt
     2041 agtccgccgg ggccgctgcc atcaccagaa cgtcggcttc cggtagatgg gcgcgaactg
     2101 cgtcctccag ctccttggtc gtttcgaccc gtcggaccgt cactccgacc ggcgatggca
     2161 gagccacggg tccggaaatg agcaccacgt ccgcgccccg ctcccaggcc gcttccgcga
     2221 cgcgataacc catcttcccg ctggatcggt tggtgacgac gcgaaccgga tcgatcgatt
     2281 cccgcgtcgg tcccgcggtc accacgacgc ggcgcccgct aagcgccgcc ttcccgcgga
     2341 gcaaccgtgc ggcgtgcgcc agaatggtct cgggctcgct catccgtccg ggccgctcgg
     2401 acggcccttc ggccagggca ccgatttcgg gtcccaccag tgaaaacccg cgctcctgca
     2461 ggatcgcgag gttccgctgc gtgatcgggt tggcgtacat ctcgtcgttc atcgcgggag
     2521 cgaggagcgc ccgcgcacgc cgggccagga gcaacgaggt gagaagatcg tcggcgagtc
     2581 cgagtgctgc ccgggcgatc aggttcgctg tggctggagc cacgatgatg agatcggcga
     2641 cccggccgag gtcgatatgg gcgagcgccc cgccgcgatc ccagagggag gtcaggacgg
     2701 ggcgcccggt gagtgcctcg aacgtaaccg gccgcacgaa ttcggcggcg ctctgcgtga
     2761 gcaccgcgtc gaccatggcg cccgcttcga cgagccgccg ggcgagaaag cagctcttat
     2821 agcacgcgat cccgcccgac acgccgagga cgacgtgccg gccggcccac acggctcagg
     2881 cctcggagcg gcggcggcgc accagcttgt agttgaggtc gcctttgacc agcgaatcga
     2941 gcgacttggt cgtgagcttc tcgccctgcg tcacgatccg gtcgcgcggc agctcattga
     3001 ggtagcgagc gaactttgcg gctaccaata ctccgagata cttgttgccc gcctgtttcg
     3061 cgacgtcgtg cggtgtggtg atgcgcatct ggtctcctta aaaagccgct gctatccctg
     3121 atctctggcc cgctggatct cccgccgcag gccggtgacg aactcgctca gcccatcctg
     3181 ccgagccacc cgttcagctt cggcgtcgag aattgccgat acccgggcca cggcatgatc
     3241 ca
//
LOCUS       DASRJQ010000094         2005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3321432, whole genome shotgun sequence.
ACCESSION   DASRJQ010000094 DASRJQ010000000
VERSION     DASRJQ010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3321432"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VFB89_02155"
     CDS             <1..501
                     /locus_tag="VFB89_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26135.1"
                     /translation="RIGAEAPAAVIPAGTKRRRWTFSFPQLAAACLAAAAISGGTVWL
                     AVAARAGTPVAVQPMAAPQAAPISPGGAAVVPASQTTAAEQKYDAAVADLQRVLEQGR
                     GTLDTTTVRVLERNLALIDRAIADARRALAADPNNSYLNAHLAQTMRRKIDLLRQAAD
                     LVAARS"
     gene            516..1394
                     /locus_tag="VFB89_02160"
     CDS             516..1394
                     /locus_tag="VFB89_02160"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HZM26136.1"
                     /translation="MLLVLAVSASLFASDTDTTITVQRGQRLELSTHSGDVVIHTWAR
                     SAVRVQTSGSSRSHLAIDQTASVINIHSAGGRGGPPNVDYTLTIPSWMAVRLSGVTAE
                     MRLEGVQGSVSAETVEGDVVVVGGGGLVSLRSVEGEVSLTDAKGKIDVNSVNSDVKVR
                     NASGELQAETVNGDVNLIQVQSDNVEATTVNGDINYDGTIRANGRYRMTTHNGDVTIV
                     IPEGTGAQISVSTFQGDFESAFPVTLTERHGKRFDFTIGNGSARIELESFGGTIHLVR
                     PGGLHVTTEKDREKEE"
BASE COUNT          394 a          624 c          637 g          350 t
ORIGIN      
        1 cggatcggag ccgaagctcc ggcggcggta atcccggctg gaacaaagcg gcgtcgttgg
       61 actttttcgt tcccccagct tgcagcagca tgcctggcag cggcggcgat ttcgggtggc
      121 acagtttggc tagccgttgc cgctcgagca ggaacacccg tcgccgtgca accgatggcg
      181 gcgccgcaag cggcaccgat atcccccggg ggggcggctg tcgtgccggc aagccagacg
      241 accgcggcag aacagaagta cgatgccgcc gtggccgacc tgcagcgcgt gctcgagcag
      301 gggcgaggca cgctggacac caccacggta cgggtcctcg agcggaatct cgccctcatc
      361 gatcgagcga ttgcagatgc gcgccgagcc ctcgccgcgg atcccaataa cagctacttg
      421 aacgcgcacc tcgcgcagac tatgcgtcgc aagatcgact tgctgcgcca agccgcggat
      481 ttggtcgcgg cccggagctg aaaggagcct ctcccgtgtt gcttgttctg gctgtcagcg
      541 ccagcctgtt tgcctcggat accgatacca cgatcaccgt gcagcggggg cagcgcctcg
      601 agctgagcac ccattccggt gacgtggtaa tccatacctg ggcccgcagc gcggtgcgag
      661 tgcagacgtc gggctcgagt cgctcccact tggccatcga tcagacagcc agtgtcatca
      721 acattcatag cgcgggcggg cgcggtggtc cacccaacgt ggactacacg ctcaccatac
      781 ccagttggat ggctgttcgg ttgtccgggg tcaccgccga gatgcgattg gagggcgttc
      841 aaggatcggt cagcgcagag accgtagagg gagacgtcgt agtggtcggt ggcggtggac
      901 tcgtgtcact ccggtcggtg gagggggaag tctcgctcac agacgccaag ggtaagatcg
      961 acgtgaattc ggtgaactcc gacgtcaagg tgcgcaatgc cagcggtgag ctgcaggcgg
     1021 aaacggtcaa tggcgacgtc aacctgatcc aggtgcaatc cgacaacgtc gaagccacta
     1081 ccgtgaacgg cgacatcaat tacgacggga ccattcgtgc aaacggccgg tatcgcatga
     1141 cgacgcataa cggcgacgtg acgatcgtga tccccgaggg cacgggcgca cagatctcgg
     1201 tttcgacctt ccagggtgac ttcgagtcgg cttttccagt gactctcacg gagcgccacg
     1261 gcaagcgatt cgatttcacc atcggcaacg gcagcgcccg aatcgagctg gagtctttcg
     1321 gaggtaccat ccacctggtc cggcccggcg gtctccacgt cacgacggag aaggaccggg
     1381 agaaagagga gtagcggcta ctccgatcag tcccgccgca atttgacaga cggtggactg
     1441 acccggggtc cagacgcgtt gagcctgtct agaatctagg cccaagctca ccaggtcttc
     1501 gctggccgat tcccatcgcc agccgacgag cctcaagctg gccgggcccg gtcatctccg
     1561 acctctgcgc cggactcggg atcgcccaac cactgccact caagccgttg cggcatagag
     1621 cagtatgcca atcaggctaa ctcaaatcag ctcatcggtc accgtagtcc ccggccgagc
     1681 agactccggc aggtgatccc tgcagcagga cagtttctcc gaacgaaatg gccccgtggt
     1741 gttcgattgt cagacattct tgcaaaaggt gtgatagatg gcacgctcct tgttcctgcc
     1801 ccgcggcacg gcggagtgtc tcagcctcag gataccggca ggtgaggcca aacctttagc
     1861 ggcggagtcg gcgatgaagc gttcaacgaa gggtatgctt tggggcacgg cagcggcggt
     1921 gcttcttgga accagtcccg cagtcgccca ggtcggtctc acctcgtcgc ttgcgacggt
     1981 gaacatcaac gcgaccaaga gctca
//
LOCUS       DASRJQ010000095         2490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1340890, whole genome shotgun sequence.
ACCESSION   DASRJQ010000095 DASRJQ010000000
VERSION     DASRJQ010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340890"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..743
                     /locus_tag="VFB89_02165"
     CDS             183..743
                     /locus_tag="VFB89_02165"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZM26137.1"
                     /translation="MGLFRHLLGHSIFPPRYAWFLEGPWRRLVLSPQRLERRLALSPQ
                     LTVLEIGTGGGYYARPLSALVHRLIAVDLQAEMLHRLQSKATSPLLLPVRADATHLPL
                     AGSSIDVVLAVTVLGEVPSVQRTIAEVRRVLRPGGVLSVSEHWPDPDFLAFSLVNELC
                     RESGFQLITRYGARFSYTANFRTNAA"
     gene            824..1678
                     /locus_tag="VFB89_02170"
     CDS             824..1678
                     /locus_tag="VFB89_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZM26138.1"
                     /translation="MFAIALVASLAIASQSLDQGLVGPDTVVVQSGGLRLHALFWRPQ
                     GKGPFRAVLFNHGSGVDSANSANGRRDVRNREQGAAVLGPTFARHGYVFLFLFRRGAG
                     LSAGQGSYSGDLRAAELAANGQEAHDRLQMRLLEGNELSDARAGLAYLRSRPEVDPRR
                     VAVVGHSFGGSLTLLLAERDSTLRAAIDFASAAGSWRSEALRARLGAAVSRTKIPVFF
                     IHASNDYSIDPGKSLAASMARLAKVHRLRIYPAFGQTPEEGHSIVYLNTAAWEADVFT
                     FLNAYMQP"
     gene            1919..2329
                     /locus_tag="VFB89_02175"
     CDS             1919..2329
                     /locus_tag="VFB89_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26139.1"
                     /translation="MPRIQRFTLGLTLLNVVLFVVLILSQASPVQGRQTELPVLRGRG
                     LEIVDAQGRIRASIRINPPSGPDSGSVETVLLRLITERGRPSVKIGSSERGSGLSFAG
                     PTGTSETYVVLKAEGTSAQLKMRAEDGREQIVKP"
BASE COUNT          414 a          802 c          787 g          487 t
ORIGIN      
        1 ggaccggccg ggcgagctgg cgctggagct ctggagcttc cccggtaggg acctgacgct
       61 gcacgggcgg ctcccgttcc gtgtgtcgcc tgcggcaggc gattccggga gtgcgaagtc
      121 ttcgggtgca cgagcgcggc cgagacgatg acccccacgc gcctccgcct actaatcctc
      181 gcatgggcct gtttagacac cttctcggcc attcgatctt cccgccgcgc tatgcgtggt
      241 ttctcgaggg cccctggcgg cggctggtcc tcagcccaca gcgcctggag cggcgcttgg
      301 cattgtctcc tcaactgacc gtactggaga tcggtactgg cggtggctat tacgcacggc
      361 cgctatcggc cttggtgcac cgactgatcg ccgtggatct gcaagctgag atgttgcacc
      421 ggctccagag taaggccact agcccgctcc tcttgccggt ccgagcggac gcaacccacc
      481 tccctctcgc ggggagctcg atcgacgtgg tgctcgccgt taccgtactg ggggaggtac
      541 cctcggtaca acgtacgatt gccgaggtcc gccgagtatt gcggccaggt ggtgtcctgt
      601 ccgtatcaga gcactggccg gacccggatt tcctcgcgtt ctcgctcgtg aatgagctct
      661 gccgcgagag cgggtttcag ctcatcaccc gatatggggc ccgctttagt tacaccgcca
      721 atttccggac caacgcggcc taacaagggc ttgctgttgg cggggcgtca gtgcctccgt
      781 agcagaagcg cgagtcgtta ggccgcacac cacagagagc cacatgttcg cgatcgccct
      841 tgtcgcttct cttgccatag cgtctcaatc gttggaccag ggccttgttg gtccggatac
      901 ggtcgtcgtc cagagcgggg gactcagact ccacgcattg ttctggcggc cgcagggaaa
      961 gggaccattc cgggcggtgc tgttcaatca tggcagcgga gtcgacagcg cgaactcggc
     1021 caacggtcgt cgtgatgtac gcaaccgtga gcagggagct gcggtactgg gaccgacctt
     1081 cgcgcgccac gggtatgtgt tcctcttctt gttcaggcga ggagctggcc tttcggctgg
     1141 tcaaggcagc tacagcggag atctccgcgc cgccgagttg gccgcaaatg gccaggaggc
     1201 acacgaccgc cttcagatgc gtctcctgga gggcaacgag ctgagcgacg ctcgagccgg
     1261 tctcgcgtac ctgcgctccc gaccggaggt tgatccgcgt cgcgtggcgg tcgtggggca
     1321 ttcgtttgga ggctcactaa cgttgcttct cgctgagcgc gacagcacgc tgcgcgcggc
     1381 gatcgatttc gcgagcgcgg ccggcagttg gcgctcggag gcattgcgcg ctcgcctggg
     1441 tgctgcagtg tcccgtacga agatccccgt cttctttatt cacgcgtcca acgattactc
     1501 catcgacccc gggaagtccc tcgctgccag catggcgcgg ctcgccaagg tacatcgcct
     1561 ccggatctac ccggccttcg gccagactcc ggaagaaggg cacagcattg tttacctgaa
     1621 cacggcagcc tgggaggcgg acgtgttcac gttcttgaac gcttacatgc agccctgact
     1681 cgctccgcga cgccatgtga gcagcctaac aagcgccagg acctgccggc gccctgacgg
     1741 ctcggccgcc gcgaggtatg ttttgactct ggtgtcgctg cggcctcacc agggccccgc
     1801 agctcaagcg cagggcgtta gaccgcgcgt tccaccgacg tttttgcgcc tttaaaccgc
     1861 tggacccagt ccggcatctc atcgctggat cccccctaac gctggaagga gtcccgccat
     1921 gccgagaatc cagcgcttta ccctcggact taccctgctg aacgtcgtgc tgttcgtcgt
     1981 gctgatcctg agccaggcct ccccggtcca aggcaggcaa accgagctgc cggtgcttcg
     2041 cggtcgtggg ctcgagatcg tggacgccca gggtcgcatc cgggccagca tcaggatcaa
     2101 tcctccgagt ggccccgaca gcggatcagt tgagaccgtg ctccttcggc ttatcaccga
     2161 acgaggccgt ccgagcgtca agatcggttc gtccgagcgg ggctcgggac tcagcttcgc
     2221 gggtcccacc ggtaccagcg agacctatgt cgtcctcaag gcggagggaa ccagtgctca
     2281 gttgaagatg cgggccgagg atgggcggga acagatcgtc aagccatgaa caacatcgcg
     2341 gtctcaccag cgcttgcgcc gcagccgaaa aggatcttac tccatggcga ttgctctgtc
     2401 cccggaagcg acgaaggagc tcttgggttc tatcaaacgc tacgtcgcgg agaactttga
     2461 gcaggacatc ggcgatctca aggctggatt
//
LOCUS       DASRJQ010000096         2791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1338788, whole genome shotgun sequence.
ACCESSION   DASRJQ010000096 DASRJQ010000000
VERSION     DASRJQ010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1338788"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..>2791
                     /locus_tag="VFB89_02180"
     CDS             118..>2791
                     /locus_tag="VFB89_02180"
                     /inference="COORDINATES: protein motif:HMM:NF023677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin TcdB middle/N-terminal domain-containing
                     protein"
                     /protein_id="HZM26140.1"
                     /translation="MDIACRVNGLPEQFVGLSNGTGVTFSIFGQLSCDLGETTGAIDF
                     DGDGKDDWYCIGQTYSNLLVFPSTGSSFLYPAFGSLDNNFCSAANYILGDLNGDGRTD
                     AACTNGRVALSTGRYFDIQAGGGTESWCASGSTFAADVDGDGASELLCNNAAAPTNDI
                     EVRKWKAGALAAAEVWKANWCAATVHAGDFNGDGKTDLICSAFSAPVVAGTGGFRADL
                     LGAAGNGIGGTTQVSYSSSVVFPSTNNPPPKQVVTALTTLDGRGGSSTATYSYSGGLM
                     DRQERRFLGFHEVVQTLPCLPGEETCPSIRTVLKQDLASAGKPESVERKDGSGHTLLR
                     SELTYATTLTPPRTSQVSEEWSYTYDVLGAYRRTRAAHQFDAYGNRTQTIFFGDADVS
                     GDEKTTAWTFQPNLTAYITDRVNSETQFAGEGTTGLKLSERHFAYDEELGWGNPPSQG
                     FLTRIDSWLDTESRWVSRKSHYDTWGNVASSLDETDRPVTFGYDSAYHVFLTSTTNGA
                     SESETAVWDPICGVPTERRDANAQPVTFQTDNYCRPTQTNFPLGAFEIRSYPSLGDPA
                     AQQVRVETPSATEGDGSGNDYVLEYFDGLGRPYKAVKKGTAAHPTIRKDTSFNARGSV
                     SSETAPYFDGFETARTTTFTFDSLDRITASTFPGNAQVQRSYGIWRDTVFDEHGHPTT
                     TELDAYGRRVKLEQVLNSQTHSTVYSFDLLGRMTGITDPAGIQWSWTFDSLGRNTARN
                     DPDSGAWTFQYDDAGRLMHQTDAKGQETDFSYDLAGRLSSKVNAAGTVTIGHSEARAG
                     YYNIGRTTSVTSAGQSLTMDYDKEGRPLQQRRMLDGVEYLALRRYDSAGRLRGITYPD
                     GEAIGSDANPLTYDRTGHLYSIPGILSEVLYDAAG"
BASE COUNT          556 a          910 c          841 g          484 t
ORIGIN      
        1 acgtggggat cggcgaacgg catctcctac gggggtcagc cccattcgtg caggatcgcg
       61 cctccctcgt atccatacgc caccgttacc tccggggact tcaacggcga tgggctcatg
      121 gacatcgcct gccgcgtcaa cgggcttccg gagcagttcg taggactctc gaacgggaca
      181 ggcgtgacct tctccatctt cggtcaactg agctgcgacc tcggcgagac gaccggggcc
      241 atcgatttcg atggagatgg taaggacgac tggtattgca tcggccagac atacagcaac
      301 ctcttggtct tcccttctac aggctcgagc ttcctctatc ccgccttcgg cagcctcgac
      361 aacaacttct gcagcgcggc gaactacatc ctgggcgatc tcaacggaga tgggcggacc
      421 gacgcagcct gcacaaatgg gagggtcgct ctttccaccg gacgctactt cgacattcag
      481 gctggcggcg ggaccgaaag ctggtgcgct tcaggcagca catttgccgc cgatgtcgac
      541 ggagacggag ccagcgagct tctctgcaac aacgcagctg ctccaacaaa cgacatagag
      601 gttcgcaagt ggaaggcggg tgcgctcgct gctgccgaag tctggaaggc caattggtgc
      661 gccgcgacgg tccacgccgg cgactttaac ggcgatggaa agaccgacct catctgttcg
      721 gctttctcgg ccccagtcgt cgccggcacc ggcggtttcc gcgcagatct cctgggtgcc
      781 gcggggaatg gcatcggcgg cacgacccag gtgagctatt cgagctcggt cgtcttcccc
      841 agcacgaaca atcctccacc caagcaggtg gtcaccgcgc tgaccacgtt ggatgggagg
      901 ggtggctcgt ccacggccac ctactcctat tccggcggac tgatggaccg ccaggagcgg
      961 cggttcctcg gcttccacga agtcgtccag accctgccgt gcctgccagg cgaggaaacg
     1021 tgtccttcga tccgcacagt cctcaagcag gatctcgctt cagccggcaa gcctgagagc
     1081 gtggagagga aagacggatc gggacacact ctcctgcgca gcgagctcac ctacgccacg
     1141 acccttaccc cgccccgcac gtcgcaagtg tccgaggaat ggtcgtatac ctatgatgtt
     1201 ctcggcgctt acaggcgtac gcgggccgcg catcaattcg acgcttacgg caaccgcact
     1261 cagacgatct tctttggcga tgctgacgtc tcgggcgacg agaagacgac ggcctggacg
     1321 ttccagccga acctcaccgc ctacatcacg gatcgcgtca actcagagac gcagtttgct
     1381 ggggaaggca cgaccggtct caagctctcg gagagacact ttgcgtatga cgaagagctc
     1441 ggatggggta atccgccgag ccagggcttc ctgacccgga tcgacagctg gttggacacg
     1501 gaatcgcgct gggtcagccg gaagtcgcat tatgacacct ggggaaatgt cgcgtcgtcg
     1561 ctggatgaga ccgatcggcc ggtgacgttc ggctatgact cggcctacca cgtgtttctg
     1621 acctcgacca cgaacggggc cagtgagagc gagaccgcag tctgggaccc gatctgcggt
     1681 gtgcccaccg agcgccggga tgcgaacgcg cagccggtca ccttccagac cgacaattac
     1741 tgccggccga cccagacaaa cttccctctc ggcgccttcg agatccgctc ctatccgagc
     1801 ctcggcgatc ccgctgccca gcaggtgcga gtcgaaacgc cttcggccac cgaaggggac
     1861 ggcagtggaa acgattacgt tctcgaatat ttcgacggcc tcggcaggcc ctacaaagcg
     1921 gtgaagaagg gaacggccgc ccatccgacg atccgcaagg atacgagctt caacgcgcgc
     1981 gggtcggtga gttccgagac ggccccctac tttgacggct tcgagacggc caggaccact
     2041 accttcacgt tcgacagcct agaccggatc actgcgtcta cgttccccgg caatgcccag
     2101 gtgcagagga gctatggcat ctggcgggac acggtcttcg acgagcacgg ccaccccaca
     2161 acgaccgagc tcgacgcgta cggccggagg gtgaagctgg aacaggtcct caacagccag
     2221 acgcactcta ccgtctacag ctttgacctc cttgggcgga tgactgggat caccgatccg
     2281 gccggcatcc agtggtcctg gacattcgac tcgcttgggc ggaataccgc gcgaaacgac
     2341 cctgattccg gtgcgtggac cttccagtac gacgacgcag gccggctgat gcaccaaacg
     2401 gacgccaagg gacaggagac cgactttagc tacgatcttg ccgggcggct ctcctcgaag
     2461 gtcaacgcgg ccggcactgt gacgattggg cacagcgagg cccgcgctgg gtactacaac
     2521 attggccgga cgacgagcgt gaccagcgcc ggtcaatccc tcaccatgga ttacgacaag
     2581 gaaggaaggc ccctccagca gcgccgcatg ctcgacggtg tcgagtacct cgctctgagg
     2641 cggtacgact cagccggccg cctccgcggc atcacctacc cagacggcga ggccataggc
     2701 agcgacgcaa accctttgac ctacgatcgg actggccacc tctattccat ccccggaatc
     2761 ctttcggagg ttctctacga tgcggccggt c
//
LOCUS       DASRJQ010000097         5909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_776420, whole genome shotgun sequence.
ACCESSION   DASRJQ010000097 DASRJQ010000000
VERSION     DASRJQ010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5909
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776420"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..439
                     /locus_tag="VFB89_02185"
     CDS             191..439
                     /locus_tag="VFB89_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26141.1"
                     /translation="MSSAIREARLRQEFGKLYPGLSPGTWLPAREVAEHIVDVVRREG
                     GRLGLHGRLIADEHFEFRGGESDRPRGRNERREDLSGS"
     gene            441..1565
                     /locus_tag="VFB89_02190"
     CDS             441..1565
                     /locus_tag="VFB89_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZM26142.1"
                     /translation="MYFKQMLDERCGCASYLIASRKSLEAAVIDPAVTIDQYEALLQE
                     RNFKLRYVVDTHVHADHVSGARQLATRHKAEVCLHESARVNYPFLPLKDGQEIELGQL
                     RLKVVHTPGHRPELISLLVINPPRSPEPSMVLTGDSLLVGDVGRPDFGGGDPEAQFHS
                     IGRLLRLPDWVSVFPGHFEGPCGKGMCGRPSTTIGFERLFNPVLHLDHGAFVAAISSG
                     IPPRPLNMVAIEATNRGTADLPWAMLTSAPPIDHVGIDAIQNGDQNAVIIDVREPEEY
                     QTGHIPSAINLPQAELASRLSELPRDRPLLMICQSGMRSVRSAQFLKQAGFSRVASVT
                     GGTSAWSAAARPLVTGELPIKPLRVVESLWSHAGASLSPG"
     gene            complement(1627..2496)
                     /locus_tag="VFB89_02195"
     CDS             complement(1627..2496)
                     /locus_tag="VFB89_02195"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZM26143.1"
                     /translation="MSHSAGVIQHVSDTARWVALYRAIESERPDALFHDPYARKLAGD
                     RAERIVASLPKGKRMSWPMVVRTVVLDELIQRAVTRDGVDTVLNLAAGLDSRPYRLPL
                     PPQLRWIEVDFPDMIAYKQEQLAGVKPVCTLEQVGLDLTDIPRRQELFTRIAAASNQT
                     LVVSEGLLIYLAREQVAKLAEDLAAQPSFRWWLIDIAGPRILKRMEKTWGRAVAAGNA
                     PFQFAPAEGTRFFDSHGWGEAEFRSMWEEANRLQRAEIPFAWLWSLLGRLSSERRREE
                     FRRMAGMVLLRRR"
     gene            2684..5170
                     /locus_tag="VFB89_02200"
     CDS             2684..5170
                     /locus_tag="VFB89_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26144.1"
                     /translation="MRKRWTAGAVIVGLFVVLRPAAAQQLAARVHADAAADSEQVVAN
                     DDYQAGGFHRMLLGDGYRNLWAEPVRVPVLNLSRVAGGLRVIRESTGKQTRSLELVSR
                     DGRQFRFRSADKEETLGLSPAMRKSMLASVFQDQTSALFPGAAALAASLAEAAGIPHT
                     ALGLFVLPDDPGLGKFRQKYAGMLGILELYPSATGAGAPGITNFRDVIKTEELAERLN
                     KSTADRIEPRPYLAIRLFDMFLNDADRHPGQWRWGTRDRSAPRQWVAIPLDRDNAFSS
                     YGGMVASVGRLGEPQLVPFTGDYRLRGLTRKASDMDARLLAELDRPVWDSIAAALTAR
                     LSDSAIAHAIRHLPTSYYQLVGADLETKLRSRRDKLSDIASRVYERLARVVVVHGTDG
                     AERVEIRPQADGSVNIQLLSLDRPQDPPYFIRHFVPDETDEVRVYLHGGADQVDTASA
                     GAARIKVRVITGEVLAPIDALDRRPWRRVPDGRIVPPQPDRDHRLVPLIRAHTAGFRT
                     GLDLGLALRHYGFERSPWGREVSATLGYSLPANAWGIAGALDLTHRDSPLFFRLSTMV
                     SDLQRPYFYGFGNQTPRGSSSDAHRVSHREYAAFAEFGLRGERWSVSAGPVVKYSTNS
                     DPKSALVGTPLGSDNYGEAGLRGRAQLDTRANSRRTSGGLRVAATADLYPKLWDSKGT
                     VGTVDLDARLYVPIVRLPLEPVLALKAGARRSWGPYAWFDASFVGGKTTLRGYDHDRF
                     AGDASFYGGADLRLLVTRFARPVPGELGVLGLFDAGRVWATGENSDRWHHAWGGGVWT
                     SLIDPSVVFAAILARGEERTALYLTLGYPF"
     gene            complement(5174..5578)
                     /locus_tag="VFB89_02205"
     CDS             complement(5174..5578)
                     /locus_tag="VFB89_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZM26145.1"
                     /translation="MARILVIDDDAMVRHGLVRVLMNAGYEVSDTASGADGLRLCREQ
                     GADLVLTDILMPNMSGTDVIRELRRVAPRLPVIAMSGGERSRDFEQLSNAGELGAVSL
                     LRKPFTREELLAVVAAALRNPARGDVDRPVPT"
     gene            5723..>5909
                     /locus_tag="VFB89_02210"
     CDS             5723..>5909
                     /locus_tag="VFB89_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26146.1"
                     /translation="MPGRSRLLAVCVLLAACGGETMGPSSPLPTEPIDLSADWAVSSP
                     EKEGVDPTKLETAFASGV"
BASE COUNT         1004 a         1910 c         1888 g         1107 t
ORIGIN      
        1 gggaccagct cgaggccggg cgagcggcca cgcggcgtgc cttcggccgg ttgtcatgga
       61 tcatcggtgc tgctttactg gccagcttgg gcgccttggc tgcagcactt cttcggtaga
      121 tcgacccggg tcttgcgaat cccggaaacc gggtacagcc tccggacatc cccaaaaccg
      181 gctgatcgat atgtccagcg ctattcggga agcgcgcctg cgccaggaat tcggcaagct
      241 gtatcccggg ctgtcgccgg gcacctggct ccccgcgcgt gaagtggccg agcacatcgt
      301 ggatgtggtg cgacgggaag gcggccgcct tgggctccac ggccgtctca ttgccgatga
      361 acacttcgag tttcgcggcg gcgagtccga tcggccgagg ggccggaacg agcgacgcga
      421 agacctcagc gggagctgac atgtacttca agcagatgct ggacgagcga tgcggctgtg
      481 cttcttacct tattgcttca cgcaagtctc tcgaagcggc cgttatcgat cctgcggtca
      541 cgatcgatca gtacgaggcc ctgcttcagg agcgaaattt caagctgcga tacgtggtcg
      601 acacccacgt gcatgccgac catgtctcgg gcgcccggca gttggccaca cggcacaagg
      661 cggaggtgtg cctccacgaa tcggcgaggg tcaactatcc ctttctgccg ctcaaggacg
      721 ggcaggagat cgagctcggt cagctccggc tcaaggtagt tcacacgcct ggacatcgac
      781 cggagctgat ttccctgctg gtcatcaacc caccgcgcag tccggagccg tctatggtgc
      841 tcacaggcga ctcgctgctg gtgggcgacg tggggcgccc cgatttcggt ggcggcgacc
      901 cggaagccca gttccatagc attggtcgcc tgctgaggct gccggactgg gtctcggtat
      961 tcccgggcca tttcgagggg ccgtgcggta agggcatgtg cgggcggccc agtacgacga
     1021 tcggattcga gcggctgttc aacccagtcc tgcacctcga ccatggggca ttcgtcgccg
     1081 caatttccag cggaattccg ccgcggccgc tgaacatggt tgctatcgag gcgaccaacc
     1141 gcggtactgc ggatctcccc tgggccatgc tcacatcggc gccgcccatc gatcacgtcg
     1201 ggattgatgc gattcagaac ggcgatcaga acgcggtcat aatcgacgtg cgggagccgg
     1261 aggagtatca gactgggcac atacccagtg cgatcaatct cccccaggca gagctggcct
     1321 cgcgattgtc ggaattgcca agggaccgac cgctattgat gatttgccag tcggggatgc
     1381 ggtcggtgcg ctcggcccag tttctcaagc aggccggctt ttcccgggtg gcgagcgtca
     1441 ccggagggac gtcggcctgg tcggcagccg cccggccgct ggtcacggga gagcttccca
     1501 tcaagccgct tcgtgtcgtg gagtcgctct ggtcgcacgc cggcgccagc ctgtcgccag
     1561 gctgagctcc gcctggtagg cgaaccgcgc cactcgctcc cggatcacac ggcagcggcg
     1621 cgcactctag cgccgacgta acaggaccat tcccgccatg cggcgaaact cttcccgccg
     1681 ccgctcggag ctgagccgtc ccaacagact ccagagccag gcgaacggaa tctctgctcg
     1741 ctggagccgg ttggcctcct cccacattga tcggaactcg gcctcgcccc acccgtggct
     1801 gtcgaagaac cgggtgcctt ccgccggcgc aaactgaaag ggggcgttgc ccgccgctac
     1861 agcacggccc caggtcttct ccattcgttt gagaattcgt ggcccagcaa tgtcgatcag
     1921 ccaccagcgg aagctcggct gagccgcgag gtcctcggcc agcttggcga cctgttctcg
     1981 ggccaggtag atcagcagcc cttcgctgac cacaagcgtt tggttcgatg ctgcggcgat
     2041 tctggtgaag agctcctgcc gccgcgggat atcggtcagg tcgagcccca cctgctcgag
     2101 cgtgcagacc ggtttgacac ccgccagctg ctcctgcttg taggcgatca tgtcgggaaa
     2161 atccacttcg atccaacgaa gctgcggcgg cagcggcagc cgatacggac ggctgtcgag
     2221 gccggccgcc agattgagca cagtgtccac cccgtcacgc gtgaccgccc gctggatcaa
     2281 ctcatccagc accaccgtcc ggaccaccat cggccacgac atgcgcttgc cttttggcaa
     2341 ggacgcgacg attcgctcgg cccggtcccc cgcgagcttg cgagcgtacg ggtcgtgaaa
     2401 caacgcgtcg gggcgctctg actcgatcgc tcgatagaga gcgacccatc gagcagtgtc
     2461 ggatacgtgc tgaatgacgc ctgctgagtg gctcaccgtg gtacctccgg ccgggaatgc
     2521 ccgaatcttg taccgcctgg tactcggcgt cgagatcctg gcagtgacct tccccacgtt
     2581 cagctggtgc gcctgaccgc ctggctcatc ctgtgatacc gcctaccgca gccagccccg
     2641 atttgaacaa gccttggatc aatagcctga accaggacag atcgtgcgca aacgctggac
     2701 ggcgggggcg gtgatcgtgg gtctgtttgt ggtgttgcgc ccggcagcgg cgcagcagct
     2761 ggcggcgcgt gtgcacgctg acgcggccgc ggactcggaa caggtagtag ccaacgatga
     2821 ctatcaagcg ggcggtttcc accggatgct tctcggcgat ggctatcgaa atctttgggc
     2881 cgagcctgtg cgtgttccgg tcctgaattt gagccgggtc gccggcgggc tgcgcgtgat
     2941 tcgggagagc accggaaagc aaacccgctc gctcgagctg gtctcccgcg atggtcgaca
     3001 attccgcttt cgctccgctg ataaggaaga aacactgggc ctgtcgccgg ctatgcgaaa
     3061 gtcgatgctg gcttcggtat ttcaggatca gaccagcgct ctctttcccg gcgcagcagc
     3121 ccttgcggcg tcgctggcgg aggcggccgg gattccccat accgcactcg gcctgttcgt
     3181 tctgcctgac gatcccgggc tgggcaagtt tcgacagaag tatgccggca tgctcggcat
     3241 tctcgagcta tatccttccg ccacgggggc cggggctccg ggcatcacca acttccgcga
     3301 cgttatcaag acggaagagc tggccgagcg tctcaacaag agcactgccg accggatcga
     3361 gccgcggccg tatctggcca tccggctatt cgacatgttt ctcaacgatg ccgatcggca
     3421 tccgggacag tggcgctggg gcaccagaga tcgaagcgct ccccgccagt gggttgccat
     3481 cccgctcgat cgggacaacg cgttctcgtc ctacggcggg atggtggctt cggtgggccg
     3541 actcggagaa ccgcagttgg tcccctttac cggcgattat cggcttcgtg ggctcacacg
     3601 gaaggcgagc gatatggacg ctcggctact agcggagctc gatcgcccgg tctgggattc
     3661 gatcgctgcc gccctgacgg cgcgcttatc cgattccgcc attgcccacg cgatcaggca
     3721 cctgcccaca tcgtactatc agctcgtcgg ggccgatctc gagaccaagc ttcggagccg
     3781 ccgtgacaag ctgagcgaca tcgcgtctcg cgtctacgag cgacttgcgc gggtggtggt
     3841 cgtgcacggg accgacggcg ccgaacgggt ggagattcgc ccgcaggcag acggatcggt
     3901 gaatatccag ctcctgtctc tggatcgtcc gcaagaccct ccctacttca tccgacattt
     3961 cgtgccggat gagaccgatg aagtccgagt gtacctgcac ggtggagccg atcaggtaga
     4021 cactgcgtct gcaggagcgg ctcgcatcaa ggtgcgagtg atcaccggtg aggtcttggc
     4081 gccgatcgac gcactcgacc gacgtccctg gaggcgagtc cctgacggac gcattgtccc
     4141 gccgcagccg gatcgtgatc acaggcttgt tcccctgatc cgggcccaca cggccggctt
     4201 tcgaaccggg ctggacctcg gtttagcgct gcggcattac ggattcgagc gatcgccctg
     4261 ggggcgcgaa gtgtccgcga ctctgggtta ctcccttccg gccaatgcat ggggcatcgc
     4321 gggtgcactg gatctcactc atcgagacag tccgctcttc ttccggctct ccacgatggt
     4381 atcggacctt cagcggccct acttctacgg attcggcaac cagacgcctc gggggtcttc
     4441 ctcggacgct catcgcgtct cgcaccgtga gtacgcagct tttgccgagt tcggactacg
     4501 cggcgagcga tggtcggttt cggcggggcc ggtcgtcaag tactcgacca actcggaccc
     4561 gaagtcggcg ctggtcggaa ccccgttggg aagcgacaac tacggcgagg caggcctgcg
     4621 gggaagagca cagctggata ccagggcaaa ttcccggcgc accagcggcg gcctccgcgt
     4681 cgcggcgact gcggacctct atcccaagct gtgggacagc aagggcacgg tcggcactgt
     4741 cgatctggac gcccggttgt acgtgccgat cgtgcggctt cccctcgagc cggtgctcgc
     4801 actcaaggcc ggggcccgca gatcgtgggg accctatgcc tggttcgatg cctcgttcgt
     4861 aggggggaaa accaccttgc gtggctacga ccacgaccgt ttcgccggcg acgcgtcgtt
     4921 ctacggaggg gcagacctcc ggcttctggt gactcgtttc gcccgcccgg tgccgggcga
     4981 gcttggcgtg ctcggattgt tcgatgcggg ccgtgtctgg gcgaccggag agaattccga
     5041 tcgatggcac cacgcatggg gaggcggcgt ctggaccagc ctgatcgatc cgtctgtggt
     5101 ttttgccgca attctggcga ggggggagga acggacggcg ctctatctga cgctgggata
     5161 cccgttctaa gtctcaggtc ggcaccggcc gatcgacatc accccgagcg ggattccgga
     5221 gagcggccgc gacgaccgca agcaactcct cccgggtaaa gggcttccgc agcaggctca
     5281 ctgcgcccag ctcaccggca ttgctcagtt gctcgaagtc gcgcgagcgc tctcccccgg
     5341 acatggcgat gaccggcaag cgaggagcga cccgccgcaa ctcccggatc acgtcagttc
     5401 cgctcatgtt tggcatgagg atatcggtca ggaccaggtc tgcaccttgc tcgcgacaga
     5461 gcctcaggcc gtcggctcca ctcgcagtgt cgctgacttc gtacccggcg ttcatcagaa
     5521 ctctgaccag accatggcgg accatggcgt cgtcatcgat tactaagatc cgtgccatgc
     5581 agacctcata gtgcaatgcc aggctcgact tcgaaagggt cttctgtctg gcgtgaaagt
     5641 cggcgacgat cgcacgtcat acacgttggc cagccaagcg atccggccgc ccaatccagc
     5701 ttcacatttt tgcggagtca tcatgcccgg tcggagccgc ttgctcgctg tgtgcgtcct
     5761 gctggctgcc tgcggcggcg agacgatggg cccctcgtct ccgcttccca ccgagccgat
     5821 cgatctttca gctgactggg ccgtcagctc acccgagaaa gaaggcgtcg atccgacgaa
     5881 gctcgagacc gcgttcgcga gcggcgtcg
//
LOCUS       DASRJQ010000098         8778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_4160197, whole genome shotgun sequence.
ACCESSION   DASRJQ010000098 DASRJQ010000000
VERSION     DASRJQ010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 8778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8778
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4160197"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VFB89_02215"
     CDS             <1..791
                     /locus_tag="VFB89_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HZM26147.1"
                     /translation="LRVLIADDEPMGRQRLSRMLQAEPDVELVGACASGQEALEAIRR
                     HRPDLAFLDIEMPGIDGFGLLDALEHERAPTIVFVTAHDRYAVRAFEVHALDYLLKPF
                     DQTRLHAALNRAREQVPTTGPADAAQRMIALLEELNQRQKGELGLERLVIRTPERTFF
                     LRTEAIDWIEAGGKHVHLHVGKATHLLRGGMTALEQQLDPARFVRISRSAIVNIDRIQ
                     EIQPWFQGDHLVILQDRTRLTSTRGYREGLQRLLGKENGRRGGA"
     gene            complement(847..1302)
                     /locus_tag="VFB89_02220"
     CDS             complement(847..1302)
                     /locus_tag="VFB89_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26148.1"
                     /translation="MRSLIALSFASLLAACYSYRPVTPVGTASPETGKRVELQLTDEG
                     GRGMVSQLGPDAQTIDGDIVRSDSSSITVAVWQVEDTHNSQTHWRGEQVVVPRGAIAT
                     LRERNLSVAGTSVIGGLIAGGALVAYEAFKGSGELQGNGPTSGPGQGQQ"
     gene            complement(1383..3023)
                     /locus_tag="VFB89_02225"
     CDS             complement(1383..3023)
                     /locus_tag="VFB89_02225"
                     /inference="COORDINATES: protein motif:HMM:NF024181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HZM26149.1"
                     /translation="MRNALKVALGLALVSGAGACSDYLSGPGIDTDPNNVDKLTRPGA
                     LYFGIQAAQVVQREGQLARNAVQYVQQVSGNSRQSIGFDRYTTKPQDMDTYFGAVYGS
                     TRTLTGGGGLLDVRKMTQIARKLNDSLYVGLAKVYEALIMGYAADLWGDVPYREAADS
                     TTLTPHFDPQLQVYADVLAELDSAINVYLPASGPSNLGPGQDNAELIYKGREGELPTL
                     YTAVAHSLKARYYMHLAERDPSNYARALAEVGPPGINNGGPSISNPNDDFQWYADGTP
                     NGNNIWWQFSLTRADVAPGSALVNIIKRRIAGGLEDDARASFYFSCADCDGSPNDFYG
                     FRPGGATDVQVAPGADAGNGSPSGNYSFFGSFIDGSLFTNSSGNFRQQELSWAEAQLI
                     GAEATWHINCAGCDPNTVVPAAQPYLDAVRANRFYGTLDGARVQFPALGPAPASLLNI
                     MEEKYVSLFLNPEVWNDYKRTCLPALAPAVPAGSSAPGTSPIAARLPYGLTELNTNPN
                     TPTSTSSVGTPITSTSRNPNDPAPCPVLNYVNSTPLAN"
     gene            complement(3052..5010)
                     /locus_tag="VFB89_02230"
     CDS             complement(3052..5010)
                     /locus_tag="VFB89_02230"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZM26150.1"
                     /translation="MFGLHRSYRFPNPAPGSEKTSRRYDNPFFVANAALASSQVGRTF
                     GGINAEWTPLSWLKFDYSLGLDYANDERTQGWPWTTSNTTIAGAGGVGAVNAGYIRNS
                     QLDHNLTGTLSYSLSQNIKGTITAGQNLNSQTFQSRQQLGVDLIAPQPFNLTNTSTLS
                     PPYDYRQTVRLSSYFLQATADLNNAVFLTAAIRNDGASTFGASTRRNWFPKGSAAWVF
                     RRAEGSSFFSYGKVRAAYGQSGTQPLPYLLPSVFNAVTITDGGWGPAASTQLAGVGGL
                     VTRYNLPTTTLGPERVKEFEAGVDVGLLSDKADLSVTYYDQKSTDVILNLPVATSTGY
                     SEVPANAAELQNKGWEVSLNIRPITNQKFAWDLGFQWARNRGKTIDLAGVQFAPFPFS
                     GGTNGLTGIQGVAIVGQPIGVYYGTDFVRCGRGLIINDVDIDHTAGYCQGAPSGALFI
                     QADGYPTIDNVGQYVLADPNPDWTGSVRTNLRFGKVAVGGLLDVRHGGISYNGTKGAL
                     NHFGTTAESQARRQGGNVTFGQDFMPGAVAGPGANTAVPLDEAWFTNMGGVFNGPTSQ
                     FMEDGGFVKLREVSLAYTLDKPWVSRSLGFSSIELRVAGRNLVSWNDYTGVDPETSLL
                     GSASPVRGLDYFNNPQSRSFLFSLTLNR"
     gene            complement(5568..6380)
                     /locus_tag="VFB89_02235"
                     /pseudo
     CDS             complement(5568..6380)
                     /locus_tag="VFB89_02235"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     gene            complement(6494..7120)
                     /locus_tag="VFB89_02240"
     CDS             complement(6494..7120)
                     /locus_tag="VFB89_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZM26151.1"
                     /translation="MNLCRRRHGGDTRILIIDDDRRPALPGTTRSWPPAVGEGLGANG
                     ATRSVRELTVPTPIPGTVPRLDLMQERGASVAADPQLVRDLVARVEQLLAQARPENRE
                     SDTSWPGTTVAAGDITLDLPLQAVSRGGRQIKVTQTEFRLLFALVRRKGIVASRQDLL
                     REVWGANPVITARVVDTHVARLRRKLEDDPKQPRHILTALSSGYRFRA"
     gene            complement(7289..7828)
                     /locus_tag="VFB89_02245"
     CDS             complement(7289..7828)
                     /locus_tag="VFB89_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26152.1"
                     /translation="MSTIEVTLCSLMVAPLLGAFPLPTPAPSHSTQLPSPCSSAEYHQ
                     FDFWIGDWQVSTPDGKRAGANTIQRTLGGCVLHENWRGASGSRGNSYNIYDASRKQWH
                     QTWVNDRGELLQLDGGIQDGRMVMTGETIDTAGKRIEHRITWSQVGKERVRQLWESSS
                     DGGRTWAVTFDGLYVRSHP"
     gene            complement(7858..>8778)
                     /locus_tag="VFB89_02250"
     CDS             complement(7858..>8778)
                     /locus_tag="VFB89_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZM26153.1"
                     /translation="LLVDRGDRVVFPVPSWNNDHYCALSGATPVPIECGKATAFLPTS
                     RMIAPALRDARMLVLNSPVNPTGTAFDAATLGAICDAVLEENERRSDDEPPLYLLYDQ
                     VYWMLTFGGLKHVVPSVLRPEIAPYTIVVDGISKVFAATGLRAGWVCPPEPLAAQFSA
                     FLGHVGAWAPRPVQIATAEMLAAAGAVEAARQTMRDGLERRLNRLHAGLLAMCNEGYP
                     VDAVAPAGAMYLSVRFGLSGGMAGSGPEATETVRRQLLERAGLAVVPFEAFGYEGEPG
                     WFRLSVGSVSEKMVEEVLVRLRETLEVRPS"
BASE COUNT         1553 a         2773 c         2861 g         1591 t
ORIGIN      
        1 cgctgcgtgt cctgatcgcc gacgacgagc cgatgggacg ccagcgcctc tcccggatgc
       61 ttcaggccga gccggatgta gagctggtcg gcgcatgcgc cagcgggcag gaggcgctcg
      121 aggcgatccg gcgccaccgc cccgatctcg cctttctcga catagagatg cctggaatag
      181 atggattcgg actcctcgat gcactcgagc acgagcgggc gccaaccatt gtgttcgtga
      241 ctgctcacga ccgctatgcg gtccgcgcct tcgaagtcca cgccctcgac tacctgctca
      301 agcccttcga tcagacccga ctgcacgccg cactcaaccg tgcacgcgag caggtcccca
      361 ccaccggacc agccgacgcc gcccagcgca tgatcgccct gctggaagag ttgaatcagc
      421 gacagaaagg cgaattgggg ctggagcggc tcgtgatccg gacaccggaa cgaactttct
      481 ttctccgtac cgaagcaatc gactggatcg aggcaggggg caagcacgtc catctccacg
      541 tcggcaaagc gacccaccta ttgcgtggag gaatgaccgc actggagcaa caactggatc
      601 cggctcgttt tgtccgcatc agccgatcgg cgatcgtcaa tatcgaccgg atccaggaaa
      661 ttcagccgtg gtttcagggc gatcacctgg taatcctgca ggaccgtacg cgactcacgt
      721 caacccgggg gtatcgagag gggttgcaga gactgctggg aaaggagaac gggagaaggg
      781 gaggggcctg aggtgcgccc ctcctgcctc cccttccccc tttccccctt tatccctttc
      841 cccgctttac tgctgtccct gccccggtcc gctggtgggg ccgttgcctt gcagctcgcc
      901 gctgcccttg aacgcctcgt acgcgaccag cgcaccgccg gcgatcaggc caccgatgac
      961 gctggtcccc gcgaccgaaa ggttgcgttc ccgcagggtt gcgatggcgc cacgaggtac
     1021 cacgacctgc tcgccgcgcc aatgtgtttg cgagttgtga gtgtcctcca cctgccacac
     1081 agccacggtg atgctggacg agtcactgcg gacaatgtcc ccatcgatag tctgggcgtc
     1141 cgggccgagc tgcgacacca tgccccgacc gccctcatcg gtgagttgaa gctcgacgcg
     1201 cttgcctgtc tccggggaag cggtgccaac cggtgttacc ggccggtagg aatagcacgc
     1261 ggcaagaaga ctggcgaacg agagcgcaat aagtgatctc attggtttgg ggagcagtta
     1321 gccggtggcc gctagccgct cgctcggagc taatggctaa cggccatggc taacagcctc
     1381 catcaattcg ccaacggtgt ggagttcacg taattcagca ccgggcacgg ggctggatcg
     1441 ttcgggttac ggctggtcga cgtgatcggc gtgcccaccg agctggtcga cgtcggcgta
     1501 tttgggttgg tgttgagctc ggtcagcccg tagggcagcc gagctgcgat aggcgaggtt
     1561 cccggagcgc tcgaccccgc gggtaccgcc ggcgccagag caggcaggca ggtccgcttg
     1621 tagtcgttcc acacttcggg gttgaggaag agcgagacgt acttctcttc catgatgttc
     1681 agcagcgagg ccggcgccgg tccgagcgcc gggaactgaa cccgcgcgcc gtccagcgtt
     1741 ccgtagaacc ggttcgcgcg aacggcgtcg aggtacggct gcgcagccgg tacgacagta
     1801 ttgggatcac accccgcaca gttgatgtgc caggtcgctt ccgccccaat caattgagct
     1861 tcggcccagc tcagctcctg ttggcggaag ttcccgctcg agttcgtaaa gagcgatccg
     1921 tcgatgaaag agccaaagaa gctgtagttc ccgctgggag agccgttgcc cgcgtcagcc
     1981 cccggtgcca cctgcacgtc ggtcgcgccc cccggccgga agccgtagaa atcgttcggt
     2041 gatccatcgc agtcagcgca gctgaagtag aagctcgcgc gtgcgtcgtc ttccagcccg
     2101 ccggcgatcc ggcgcttgat gatgttcacc agcgccgatc cgggggccac gtccgcccgc
     2161 gtcagcgaga attgccacca gatgttgttg ccgttaggcg tgccatccgc ataccactgg
     2221 aagtcgtcgt tgggattgct gatgctcggc ccgccgttgt tgatccccgg cggtcctact
     2281 tcagccagcg cgcgagcgta gttcgacggg tccctctcgg ccaggtgcat gtaataccgc
     2341 gccttgagcg agtgagccac cgccgtatac agtgtgggca gctcgccttc ccgccccttg
     2401 tagatcagct cggcgttgtc ctggcccggg cccaggttcg acggaccgct ggccgggagg
     2461 tagacgttga tagccgagtc gagctccgcg agcacgtccg cgtacacctg caactgggga
     2521 tcgaagtggg gcgtgagggt ggttgagtcc gccgcttccc gatacggcac gtcgccccac
     2581 agatcggcag cgtaccccat gatcagcgcc tcgtacacct tggcgaggcc gacgtagagg
     2641 gaatcgttca gcttccgcgc gatctgcgtc atcttgcgga cgtcgagcaa tcccccgccg
     2701 ccggtgagtg ttctggtcga gccgtacacc gcaccgaagt acgtgtccat gtcctgcggc
     2761 ttcgtggtat agcggtcgaa gccgatagac tggcgcgagt ttccggaaac ctgctgaacg
     2821 tactgaaccg cgttgcgggc aagctgcccc tcgcgttgca ccacctgggc ggcctggatg
     2881 ccgaagtaca gcgccccagg ccgggtcagc ttgtctacgt tgttcgggtc cgtatcgatg
     2941 ccggggcccg agaggtagtc gctgcaggca cctgctcctg acacgagcgc gaggccgagc
     3001 gcgaccttca gagcgtttcg catggatcga ctctcctgtg ggctcggccc gtcagcggtt
     3061 tagcgtgaga gagaagagga acgaccggga ctgcgggtta ttgaagtagt cgagcccccg
     3121 gaccggggag gcggacccga gcagcgacgt ctcgggatct accccggtgt aatcgttcca
     3181 gctgacgaga ttcctccccg ccacgcgcag ctcgatgctg ctgaagccaa gcgagcgaga
     3241 cacccacggc ttgtcgagcg tgtacgcgag ggagacctca cggagcttga cgaacccccc
     3301 gtcttccatg aactggctgg tcggcccgtt gaagacgccg cccatgttgg tgaaccaagc
     3361 ctcgtccagc ggaaccgcag tgttcgcacc cggccccgcc accgctccgg gcatgaagtc
     3421 ctgaccgaag gtcacattgc caccttgccg ccgagcctgt gactctgccg tggtaccgaa
     3481 atgattgagc gcacccttgg tcccgttgta cgagatgccg ccgtgccgga catcgagcag
     3541 cccgcccaca gccaccttgc cgaaacggag gttggtccgc accgagccgg tccaatcggg
     3601 attcggatcg gccagcacgt actgtccgac gttgtcgatg gtgggatagc cgtctgcctg
     3661 aatgaacagg gctccactcg gtgcgccctg gcagtagccg gccgtgtggt cgatgtcgac
     3721 gtcgttgatg atgagaccac ggccgcagcg cacgaagtcg gtgccgtagt acacgccgat
     3781 cggctggccg acgatggcta cgccctggat acctgtgaga ccgttggtgc cgccgctaaa
     3841 ggggaacggc gcgaattgga cgccggcgag gtcaatggtc ttgccccgat tgcgggccca
     3901 ctggaagcct agatcccagg caaatttctg attggtgatg ggccggatat tgagtgatac
     3961 ctcccagccc ttgttctgca gttcggccgc gttcgcgggg acttcgctgt agccggtgct
     4021 ggtcgcgacg ggaaggttca ggatgacgtc ggtggacttc tggtcgtaat acgttacgct
     4081 caggtcggcc ttgtcgctca gcaggcccac gtcgacaccc gcctcgaact ccttcactcg
     4141 ctcaggccca agcgtggtgg tcggcaggtt gtaccgagtc accagtccgc ccactccggc
     4201 gagctgtgtt gaggcagccg gcccccagcc tccatccgtg atcgtcacag cgttgaacac
     4261 tgagggcagc aagtaaggca gtggctgagt gccgctctgt ccgtacgcgg cgcgcacctt
     4321 cccgtacgag aagaaggagc tcccctcagc ccgacggaaa acccaggcag cgctgccctt
     4381 ggggaaccag ttccgacgag tggaggcacc gaaggttgac gcaccgtcgt tcctgatggc
     4441 cgcagtgagg aacactgcgt tattgaggtc ggcggtggcc tgcaggaagt acgacgacag
     4501 ccgaacggtc tggcgatagt cgtacggcgg cgagagcgtc gaggtgttgg tgagattgaa
     4561 cggctgtggt gcgatcagat cgacgccaag ctgctgtcgg ctctggaatg tttggctatt
     4621 gaggttctgg ccggccgtaa tggtgccctt gatgttctgg ctcaggctgt agctcaacgt
     4681 gccggtgaga ttgtggtcga gctgtgagtt gcggatgtag cccgcgttga ccgctcctac
     4741 accgcctgcg cccgcgatcg tggtattcga cgtggtccac ggccaaccct gggtccgctc
     4801 gtcgttggcg tagtcaagac ccagcgaata gtcgaacttg agccaggaca ggggcgtcca
     4861 ttcagcattg atgccgccga aggtgcgccc cacctgggag cttgccagcg ccgcgttggc
     4921 gacgaagaac gggttgtcgt aacgccggct ggtcttctcg gagcccggag cggggttggg
     4981 gaaacgatag ctccggtgga gtccgaacac cggatccaga tacggcaggt tgttgaagtc
     5041 gggcggcgta cgccaggcag cgagtgtgag gccgtcggtg ctgttcctgg tggtgaggaa
     5101 gccgccgtcg ccatcggaat aggcgacgtt cgcccaaccc tgagctgatc gaagagccga
     5161 tgggagccgt tgaaccgaac agagatccgc tggtagtggt tgttcggccc gaccacgatg
     5221 ccgcgattgt agttgtaggc accggagagg aagaacgtgg tgcggtcatt cccccccgac
     5281 atggtcaacg cattatcggt ctgccaaccg gtctcgaaca tctccgtggc gtgatcatat
     5341 gtcggcgttc ctggcgcgag ctgggggccc cagctggcgg ccgcaccaaa cgcgaccccg
     5401 cagtttgcca aggccgggtc gctgctcgag acgcaggccg gtgctgcgcc gccggtgccg
     5461 agcccgaagg tccgctggag ctcaggcagc tgcgtgtgct ggtccattga ccactggctg
     5521 cggagcgagt acttggtcgg acccgcgcgg cccttcttgg tcgtgatcag aatgacgccc
     5581 tgccctgctc gcgaaccgta gatagcgccg gatgatgcgc ccttgaggat ctctacgttc
     5641 tcgatgtcgt ctggattgat gtcgatgagg cggttgggag gggcggcgct ggaattgaga
     5701 gctgcgtttg tcggattgtt gtaattgatc ccgttatcga ccgggacgcc gtccaccacg
     5761 aagagtggct ggccatcgcc ggtgccggcg gtgatcgtgg tgagtcctcg gatctgaatg
     5821 aacgacgacg cgcccggatc acccgagctc gaggtgacgg taacgttggg cgccttggcc
     5881 gccagagctg tcacaagatt ctgctcggcg gaattctgga tcgtcgacga atcgacgctg
     5941 cttcggaccg taccaagctt ttccacctct gatacggttc cagcgccggt cactacaacc
     6001 tcgcccagct ggagcggatt gtccggcagg gcaaagtcct gttgcgccga cccagcggcg
     6061 aaactgatct ccgaggtctt ggccttgtat ccgatgaggc gggccgtgac cgcaaccggt
     6121 gaggttggta tccgtggagc ggggaccagg atcgtgtagg tcccgtctcc gcgagtcgta
     6181 gcgccggcgc cgagctgctc gatcgtcacc tgggcaccgg acaatggcgt tccggcgacg
     6241 gtcgtcactc gtccggtcag ggtggcgggc tgctgtgcca gaagtcccgc cggcaccagc
     6301 gcgaggagcg ccgcgcacag acgagttctc gcccacagtc cgccggtccc agcgatcctg
     6361 ccgaaccctg cgatgctcat aggcacctcc gtggggaagc tggaacgaca tggacgtacg
     6421 cgattgcgtg tgctccggga ttgagggctc ccggttgccc ttcaccgccg tgccccgagc
     6481 ccgcgcccga tcgtcaggcg cgaaagcggt agccggatga cagagcggtg aggatgtgac
     6541 gaggctgctt gggatcgtcc tcgagctttc ggcgaagccg ggccacgtgg gtgtccacca
     6601 cccgagccgt gatcacggga ttcgcgcccc agacctctcg cagcaggtcc tgccgggagg
     6661 cgacaatgcc cttccgcctc accagcgcga agagcaggcg gaactcggtc tgggtgacct
     6721 tgatctgcct gcctccgcgg gagaccgcct gcaatggcag gtcgagcgtg atgtcgccgg
     6781 ccgcgactgt tgtccccggc caggaggtgt cgctctcccg gttctccggt cgcgcctgcg
     6841 caagcagttg ctcgacccgc gcgaccagat cccggaccag ttgcggatcg gccgcgacgc
     6901 tggcaccgcg ctcctgcatc agatcgagac ggggcactgt cccgggaatc ggcgttggga
     6961 cggtaagctc gcgaaccgac cgcgtcgccc cgttcgcgcc caggccttca cccaccgcag
     7021 gcggccacga tcgcgtcgta ccaggcaatg ccggacgccg gtcgtcatca atgatgagga
     7081 ttcgcgtatc gcctccgtgg cggcgccgac acaagttcaa ggcacccctc gtgcacacgc
     7141 gaggatagac gacgcaggcg atgagctaga acaggtttgg aacgaagcgc cggcctgcgg
     7201 gaacgagtcg caggccggcg ggaacgaatg actgcagtga aagcgttacg gcgacgtcac
     7261 gaatgtgaac gacgcgacgc gagggtgatc acggatggga tcggacgtag agtccgtcga
     7321 atgtgacggc ccaggtgcgg ccaccgtccg aagaggactc ccaaagttgg cgcacgcgct
     7381 ccttgcctac ctgcgaccag gtgatgcggt gctcgatgcg cttgccggcc gtgtcgatgg
     7441 tctcaccggt cattaccatc cgcccatcct gtattccgcc gtcgagctgc agcaattctc
     7501 cccggtcatt cacccacgtt tggtgccact gctttcgcga tgcgtcgtag atgttgtaac
     7561 tgttgccccg gcttccgctc gcgccgcgcc aattttcgtg aagaacgcag ccgcccagcg
     7621 tgcgctgaat agtgtttgcg ccggctcgct ttccatccgg cgtgctcacc tgccagtcgc
     7681 ctatccagaa atcgaattgg tggtactcgg ccgagctgca aggactgggc aattgcgtgg
     7741 aatgggaagg tgccggtgtg ggaagcggga atgcgcccag tagcggagcg accatcagcg
     7801 agcaaagcgt cacttcgatt gtcgacacca cacgcctccg agtagggaca agtcccctta
     7861 cgaagggcgg acctcgagcg tttcccgcaa gcgcaccagg acctcttcca ccattttctc
     7921 ggacacactg ccgacggaca gtcggaacca acccggctca ccctcgtacc cgaaggcctc
     7981 gaacggcacg acggcaaggc cggctcgctc gagcagttga cgccgcaccg tctccgtggc
     8041 ttcggggccg gagccagcca tgccccctga caatccgaat cgcacgctca gatacatcgc
     8101 acccgccggt gctactgcat cgaccggata tccctcgttg cacatcgcga gcaggccggc
     8161 atgcagacgg ttgagccggc gctcgaggcc gtcgcgcatt gtctggcgag cggcctcgac
     8221 cgccccggca gcggctaaca tttcggcggt cgcgatctgc accgggcgag gcgcccacgc
     8281 gccgacatga cccaggaacg cactgaattg agccgcgagt ggctcgggcg ggcagaccca
     8341 tccagctcga aggccggttg cggcaaaaac cttcgagatg ccgtcaacca caatcgtgta
     8401 cggcgcaatt tcgggccgca gcacggatgg gaccacatgc ttgagaccgc cgaacgtgag
     8461 catccagtat acctgatcgt aaagcaggta gagcggcggc tcatcgtcgg aacggcgctc
     8521 attttcctcg aggaccgcgt cgcagatggc tcccaacgtc gcggcatcga acgcggtacc
     8581 ggtcgggttg accggtgagt tgagcaccag catgcgcgcg tcgcgaaggg cgggcgcgat
     8641 catcctggag gtgggcagga acgcggtggc cttcccgcac tcgatcggaa ctggggtcgc
     8701 tcccgaaagc gcacagtagt gatcgttgtt ccaggagggg actggaaaga cgactcggtc
     8761 accgcgatcg accagcag
//
LOCUS       DASRJQ010000099         5916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1060228, whole genome shotgun sequence.
ACCESSION   DASRJQ010000099 DASRJQ010000000
VERSION     DASRJQ010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5916
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060228"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..773)
                     /locus_tag="VFB89_02255"
     CDS             complement(168..773)
                     /locus_tag="VFB89_02255"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HZM26154.1"
                     /translation="MSGVLVPGKRGAQPAAARWLREAAESIRGIDPAAVEQAAAVLIE
                     ARRLDGTIFVAGNGGSAATASHLALDLQKAGRAQGGRGTRAISLADSMGLVTAWGNDT
                     DFDRVFAEQLEVLGRPGDALVIVSVSGSSPNLMALISTARSLGVRTVGLLGRTGGKAR
                     DLVDVAVLVPSDDYGWVESAHVVLHHALTYAVREAARAEHG"
     gene            complement(770..2218)
                     /locus_tag="VFB89_02260"
     CDS             complement(770..2218)
                     /locus_tag="VFB89_02260"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HZM26155.1"
                     /translation="MGRVWRRVRIGILATDAVAVLAGYGLATVGHQQLLARQLGGHVW
                     PVYPVLAAAVALITVGLAWGQGLYRRWALLGTYQVYPRLIGTSTYGVFGVVMLSYFLG
                     GPPFVSRSWLVMAWLGSMAGLMVGRLLWRRIIQARLGRGQWLRRVLVAGANQHGIAVA
                     RQLRDPRHGTEVVGFLDDYQRPGTVIADGLAVVDHPLRAAAVADRLDVEQIIIIGGAL
                     SWESQRHLAELVTRPDRRVEAWVSPTFYDLLTTSTEMTNVGHVPLLYLHPTRLRGWGA
                     GAKGFVDAVVALLLVVALAPAWIYWWLKARARGIPMVDRQRVMSASGAFELMGLHSGL
                     VRSPALARLPALFNVLRREMSLVGPRPIEPRELRTHERWWSSLATMRPGLTGLWRLYT
                     GDLTVEERVALDLYYVRNYSIALDAQILYLTVRGLTGRMLGTVSVLARWHPEHPSTPS
                     EGRPPMPVSLRRLSPSLGIPSPVAGRYLGSDR"
     gene            complement(2188..3258)
                     /locus_tag="VFB89_02265"
     CDS             complement(2188..3258)
                     /locus_tag="VFB89_02265"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26156.1"
                     /translation="MLIARAPLRISFAGGGTDLEAYYARYGGLVVSATINRYFYVIAT
                     RTIDDAVQITSSDFRVFERQAAGVALSFDGELGYLKSILHEFGVTRGLSVFTASEIPP
                     GTGLGSSSTVAVALIKALATLCQRRATKQEVAELASYIEIGKLGRPIGLQDQFAASFG
                     GLNAIEFSATRTVVRPLELPLLIRDQLERSLLLFYTGASRNAATILREQRASSQERDA
                     TVIESLHGIKRDAELAREALRAGDIRQIGQILHESWQRKKRLANGISNERIDRLYQLA
                     LEHGASGGKIAGAGGGGFLLLFCEPEHQPTVTQVLEAEGLTRMTFHFDDGGAQVLVNS
                     MPEIPGLEYPEGRWVGSGVASV"
     gene            complement(3260..3994)
                     /locus_tag="VFB89_02270"
     CDS             complement(3260..3994)
                     /locus_tag="VFB89_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HZM26157.1"
                     /translation="MKALLLAAGEGQRLRPLTSDRPKPMVPVAGTPMIAYGLSWLRSN
                     GVTEVAINTHYKPDAILSFVGDGSAFGLAVRYSPEPELLGSSGALAPLRGFLYGNDPF
                     IVACGDVLTDLQLAPIVAAHRESNADATIVLTRVDDPRRAGMVEVDDAGWVLRLVEKP
                     ADWTALDAWANAGIYVLGQRVWDFVPPAGFHDFAFDLFPALLAAGGRVRGIKSDALVI
                     DIGSHERLTAASSLVANGHLNSPRAA"
     gene            complement(3991..4623)
                     /locus_tag="VFB89_02275"
     CDS             complement(3991..4623)
                     /locus_tag="VFB89_02275"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZM26158.1"
                     /translation="MIANPVFLARHGETDSNRAGIYAGRRNDPLTRNGRSQVARLAEQ
                     IKSLGIRQIWSSSVWRALESASLLREHLRLPITIDQRLDEMRLGPWEGRSEAEVARDF
                     PDDYKLWLTHPDQVRLEGRELLTQVAARTMSAVADACATHLPTLLVTHVAPIRVAVLS
                     TIGCPLGSYKRVSVPNASCVRLDCAAEQATWAPHGASLRAEIEQAGPVAA"
     gene            5211..>5916
                     /locus_tag="VFB89_02280"
     CDS             5211..>5916
                     /locus_tag="VFB89_02280"
                     /inference="COORDINATES: protein motif:HMM:NF012676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HZM26159.1"
                     /translation="MATAAPVSPNATFPADAISKLFAPPEREHPAEKLRRLLHEAKAK
                     GSYLHTAGAYDAFSAAIMTRIGFKALYGSGWQLAATKSMYPDIGIYQSHQMVELVLEI
                     RKGIEGARNTHWYDTEGRELLDAPPAFVDMEAGFGGPTQTFTLATELIRVGAAGVHLE
                     NQDPTNRTCGHIVNVGKAKRDKVLVTRAAWLAKLKAIKAAAQATGIDVVIIARTDSID
                     GALPGSSAGGVKMAIED"
BASE COUNT         1107 a         1984 c         1890 g          935 t
ORIGIN      
        1 gatgactcgg cggcgatgca gcagcgggcc gggagaggtg acgacgacga gaagagaggc
       61 gaccagcagc accggcgacg cggcgatcag cacgagcgtc gcgcctacca ggtccatcag
      121 ccgcttgccg ggatgcccga cgctgggatg gcggtcgaca gcccccgtca tccgtgctcg
      181 gcgcgcgctg cttcgcgcac cgcgtaggtg agcgcatgat gcaggacgac gtgggccgac
      241 tcgacccagc cgtagtcgtc gctcggtacc aggacggcga cgtcgacgag atcccgtgcc
      301 ttgccgcccg tgcgccccaa tagaccgacc gtccgaacgc ccagcgatcg ggcagtggaa
      361 atgagcgcca tcaagttggg cgagctgccg ctcaccgata caatgacaag cgcatcgccc
      421 ggacggccga gcacctcgag ctgctcggcg aagacccggt cgaaatcggt gtcgttgccc
      481 cacgccgtga ccaggcccat gctgtccgcc agcgagatgg cccgggtgcc gcgcccgccc
      541 tgcgctcgcc ccgccttctg gagatcgagc gcgagatgtg atgccgtcgc cgcgctccca
      601 ccgttgccag caacgaagat ggtgccgtca agcctccggg cttcgatcag cacggcggcg
      661 gcctgctcga ccgcagccgg gtctatgccc cggatcgact ccgcagcctc acggagccag
      721 cgggctgcgg cgggctgcgc ccctctcttc cctggcacca gtacgccgct catcgatccg
      781 atcccaggta gcggccggca accggcgacg gaatgccaag gctgggcgac agccggcgca
      841 acgaaacggg catgggaggt cggccttcgg atggcgtgga ggggtgctcg gggtgccagc
      901 gggccagcac gctgaccgtc cccagcatgc gtcccgtcag tccgcggaca gtcaggtaga
      961 gaatctgtgc gtcgagcgcg atagagtagt tccgcacgta gtagaggtcc agcgcgaccc
     1021 gctcctccac ggtcagatcg ccggtataca accgccagag accggttagt cccgggcgca
     1081 tggtggcgag actgctccac caccgctcgt gcgtccgaag ctcgcgcggc tcgattgggc
     1141 gtggacccac caggctcatc tcgcggcgga gcacgttgaa gagcgccggc agccgagcaa
     1201 gcgcaggcga tcgtaccagg ccactgtgca ggcccatcag ctcgaacgcg ccactcgcgc
     1261 tcatcacgcg ctgccggtcc accatcggga tgccacgcgc ccgggccttg agccaccaat
     1321 agatccaagc cggcgccaac gcgaccacga ggagcaatgc caccaccgca tcgacgaacc
     1381 ctttagcgcc cgcgccccag cccctgaggc gcgtcgggtg gagatacagg agcggcacgt
     1441 ggccgacgtt ggtcatctcg gtggacgtcg taagcagatc gtagaacgtg ggcgagaccc
     1501 aggcttcgac cctccggtcc gggcgggtta ccagctctgc gagatggcgc tggctttccc
     1561 aactcagcgc cccgccgatg atgatgatct gctcgacatc gagacggtct gccacggccg
     1621 ccgcgcgcag cgggtggtcc accaccgcga gtccgtcggc gatcacggtg ccgggacgct
     1681 ggtagtcgtc gaggaacccc acgacttccg tgccgtgcct tgggtctcgc agctgtcgcg
     1741 ccaccgcgat cccatgctgg ttggctccgg ctacgagcac ccggcggagc cactgccccc
     1801 gaccgaggcg cgcctggatg atccggcgcc agagcagacg ccccaccatc agccccgcca
     1861 tgctgccgag ccatgccatc accagccagg accgggagac gaacggcgga ccgcccagga
     1921 aatagctcag cattaccaca ccgaacacgc cgtaggtgga ggtgcctatc agccggggat
     1981 agacctggta ggtgccgagc aatgcccatc ggcggtacag tccctgcccc caggcaagcc
     2041 ctacagtaat cagcgcgacc gcggccgcga gaaccggata gaccggccac acgtggcccc
     2101 ccagctgccg cgcgagcagc tgctggtggc cgaccgtggc caggccgtat ccggccagga
     2161 ccgcgacggc atcggtggcg agaatgccta tacggacgcg acgccagacc cgacccatcg
     2221 tccctccgga tactccaggc cgggaatttc cggcatggaa ttcaccaaca cttgcgcccc
     2281 gccgtcgtcg aaatggaacg tcatccgggt gaggccctcg gcttcgagaa cttgggtgac
     2341 cgtaggctga tgttccggct cacagaacag cagaagaaac cctccgccgc ccgcgccggc
     2401 gatcttcccg ccactcgccc cgtgctcgag ggcgagctga tagaggcggt cgatccgttc
     2461 gttgctgatc ccattggcca accgcttctt ccgctgccac gactcatgca ggatttgacc
     2521 gatctgccga atgtcacccg ctcgcagtgc ctccctcgca agctctgcgt cgcgcttgat
     2581 gccgtggagg ctctcgatca cagtcgcgtc ccgctcctgc gaggaggccc gctgctcccg
     2641 cagaatggtc gccgcgttgc gggaagcgcc ggtgtagaag aggagcaggc tgcgctccaa
     2701 ctggtcgcga atcaggagag gaagctcgag tggacggacc acggtgcgag ttgcactgaa
     2761 ctcgatcgcg ttcaagcctc cgaaggaggc ggcgaattga tcctgaagcc cgattggccg
     2821 tccgagtttg ccgatctcga tatacgaagc cagctcggcc acttcctgct tggtagcgcg
     2881 gcgctgacag agtgtggcga gcgccttgat gagcgcgacg gcgaccgtgc tcgagctgcc
     2941 cagccccgtg cccggcggga tttcgcttgc agtgaaaacc gacaacccgc gagtaacccc
     3001 gaactcgtgc aggatcgact tgaggtagcc cagctcgccg tcgaacgaga gcgcgacccc
     3061 ggccgcctgc cgctcgaaga cgcggaaatc cgacgacgtg atctgcaccg cgtcgtcgat
     3121 cgtgcgagtg gcaatgacgt agaagtaacg attgatggtg gcgctgacca ccagtccgcc
     3181 ataccgggcg tagtaggcct cgaggtcggt gccacccccg gcgaacgaaa tgcgcagcgg
     3241 cgctcttgcg atcagcacgt caggccgccc ggggggaatt cagatggccg ttggcaacga
     3301 gtgaactggc ggccgtgaga cgttcgtgcg atccgatgtc gatgaccagc gcgtccgact
     3361 tgatgccgcg cacccgaccg ccggccgcaa gaagcgcggg aaataggtcg aacgcgaaat
     3421 catggaatcc ggcgggcgga acgaagtccc acacccgctg tcccaggaca taaatccctg
     3481 cattggccca ggcatccagc gcagtccaat cggccggctt ctccaccagc cgcaggaccc
     3541 aaccagcgtc gtccacctcg accattccgg ctcggcgcgg atcatccacc cgcgtgagca
     3601 cgatggtcgc gtcggcgttc gattcacgat gcgcggcgac gatcggcgcg agctggagat
     3661 cggtcagaac atctccgcag gcgacgatga acggatcgtt tccgtacagg aagccccgca
     3721 ggggtgctag tgcacccgac gagcccaaca actccggctc cggggagtaa cgtacggcga
     3781 gcccgaatgc cgagccgtcg cccacgaagc tcaggatcgc gtccggcttg taatgcgtgt
     3841 tgatcgccac ctcagtgacc ccgttgcttc ggagccagga aagaccgtag gcgatcattg
     3901 gcgtgccggc gaccgggacc atcggcttcg gccggtcgga agtaagcgga cggagccgct
     3961 ggccttcgcc ggcggcgagg agaagcgcct tcacgcggcg acgggtccgg cttgctcgat
     4021 ctcagcccgg agtgaggcgc catgcggtgc ccatgtggct tgctccgccg cgcaatccag
     4081 acggacacag gaggcgttgg gaacgctgac tcgcttgtac gaaccgagtg ggcagccaat
     4141 cgtgctcagt acggccactc ggattggcgc cacatgggtg accagaagag tcggcaggtg
     4201 ggttgcgcaa gcatcggcaa cagccgacat ggtgcgcgcc gcgacctgcg tcagcagctc
     4261 gcgtccttcg aggcgaacct gatcgggatg ggtgagccag agtttgtagt cgtcgggaaa
     4321 gtcacgggct acctctgcct cgctccgccc ctcccaggga ccaagacgca tctcatcgag
     4381 ccgctggtcg atcgtgatgg ggaggcggag gtgctcccga aggagagagg cggactcgag
     4441 cgcgcgccaa accgagctgg accagatctg ccggatgccg agcgatttga tctgctccgc
     4501 gagccgagca acctgcgacc gaccgttcct ggtgagcgga tcgttccgcc gcccggcgta
     4561 gatgccggct cggttggagt ccgtttcgcc gtggcgggcg agaaagacgg gattcgcgat
     4621 caaggccgtt cctttcaaca agggcgttcc tgctcgcatc cgccgaccat cgacgggctg
     4681 acggctccgg gttcctgttg tgacttcaga gtacgcgcac cacgggcagc agctgaaaag
     4741 ttcaggatcg ctgcgtccgc cgaacggata gccctgtcac ctctcgaatg cacgcgaaag
     4801 caaggtccgt tgctctcact gtctcgtttc agtacatccg gcggcataac taaaatcggt
     4861 aactccctat ccggcaagag aatgacccga cgaatcaagc tttcgtcact gtgggccgat
     4921 ctagccacca acgcggagag ccgttggcgc gggcaagtcc ggtaccattc cggccacagt
     4981 gattgcattc accacccagt acctatcgga atggattgac acacttgcca acttttcacg
     5041 agagacgcac gggcactgcg ctcagcatca cgactctcgc tcccaccccg acgctactgg
     5101 cgcctctggc caccgttgtt tatgttgtga aataattcac aacctccggc gcgcctgggt
     5161 ccggttcgcc ggcgggtctt tttttctgag ccccacgcgg agaatccgtc atggcgactg
     5221 ccgctccggt gtcccccaat gccacgttcc ctgccgacgc gatctccaaa ctgttcgccc
     5281 cgcccgaacg ggagcacccg gccgagaagc tccgtcggct gcttcacgag gcaaaggcga
     5341 aggggtcgta cctccatacg gcgggcgcct acgacgcctt ttccgccgcg atcatgaccc
     5401 ggatcggatt caaggcgctc tatgggagcg gctggcaact ggccgctacc aagagcatgt
     5461 atccggacat cggcatctat cagtcgcatc agatggtcga gctcgtcctc gagatcagaa
     5521 agggcatcga aggcgctcgg aacacgcatt ggtacgacac cgagggtcgc gagctgctcg
     5581 atgcgccgcc tgcttttgtc gacatggaag ccgggtttgg tggccctacc caaaccttta
     5641 ccctggcaac cgagctgatc agggtgggtg ctgccggcgt gcacctcgaa aaccaagacc
     5701 cgaccaatcg gacgtgcggt cacatcgtca acgtgggcaa ggctaagcgg gacaaggttc
     5761 tggtcactcg cgccgcctgg ctggccaagc tgaaagcgat caaggctgcc gctcaggcca
     5821 cgggcatcga cgtggtcatt atcgcacgaa ccgactccat cgacggagcc cttccgggca
     5881 gctcggcggg cggggtcaaa atggcaatcg aagacg
//
LOCUS       DASRJQ010000100         3908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2225358, whole genome shotgun sequence.
ACCESSION   DASRJQ010000100 DASRJQ010000000
VERSION     DASRJQ010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2225358"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="VFB89_02285"
     CDS             complement(<1..1091)
                     /locus_tag="VFB89_02285"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZM26160.1"
                     /translation="MPIEKKLTAALADRYRIERELGAGGMATVYLAEDLKHRRRVAIK
                     VLRPGISEALAAERFLREVEIAAGLTHSHILPVYDSGQADGLLYYVMPYVAGESLRQK
                     MTREGALPVPEAARLLHEVADALGKAHRAGIVHRDVKPENILLSDGHALVADFGVAKA
                     VSEAGSKGNLTSAGMAVGTPAYMSPEQAAGDPHVDHRADLYALGVVAYEMLTGQPPFT
                     GGTPQQILAAQVTTPPPPILGHRAGLPPSLADLVMRLLAKHPADRPQSAEQLFPTLEA
                     ATTPSGVTAALPSLPSEPARRSRRWKTLAVVGVLVVAAALGILITRKRQPAHVVAPAV
                     DAARSVAVLPFTNLSGDSANEYFSDGISE"
     gene            1159..2007
                     /locus_tag="VFB89_02290"
     CDS             1159..2007
                     /locus_tag="VFB89_02290"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZM26161.1"
                     /translation="MPVFDTVRPGWHFGTRKKTSRLGRFILSENQCPPNLRTPWHGHE
                     TAAFCLVLRGRYLQRFRHRDVVYQPSVALFRPPGIEHTDQVSPEGVASFFVEPDPAWL
                     AQMGLEQLNVDYALARSGPRGRWLLEQIFSEFSSPDRASPLALEGLVLALGAEFARQP
                     EARIRRPPGWLERTREALDGSLAERLTLAALAAEAGVHPVHLAAAFRAAYGTSVGQYL
                     RARRIDAARSELLDHRRSINEIALSFGFSSQSHFTRVFREHSGMTPRAYRRLHRVMPE
                     IEVRRA"
     gene            2081..3199
                     /locus_tag="VFB89_02295"
     CDS             2081..3199
                     /locus_tag="VFB89_02295"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZM26162.1"
                     /translation="MRRVVQLPLAVALSVVVCSSAVAQSAISALPRSKVQRADAVIAD
                     YMTAHQVPGLSVAIGLDQRLIWSKAYGLADMEDSIAATTRSMFRTASIGKPMTATAVM
                     LLWQEGKLDLDAPIQRYCRAFPEKPWPVTARQLLAHRSGIRDKTPQEDSNYTHYATIE
                     ASVAIFGGDSLLFQPGTAFRYSSYNYDVLGCAIEGASGMSYMDYMNRRIFGPVGMSHT
                     LLDDPKPIIPHRVAGYALDKQGKLINSIHDDMSNRIPAGGFVSTPSDLVRFGLAVLAG
                     KLVADSTLQFMWQVQRAPTDSVTKEGYGLGWALGDWFGVQEVSHGGGTPQVCALLYLL
                     PAKRIVVAFMMNLETVPDRGDLAGDLVKVVLGPNAPHN"
     gene            complement(3201..>3908)
                     /locus_tag="VFB89_02300"
     CDS             complement(3201..>3908)
                     /locus_tag="VFB89_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26163.1"
                     /translation="ADRRPIWSPDGRTVAFLRDSGATTMLYARPADGSGEDRLVARLD
                     RQIQEALWSHDGQWLILRTDNATPGAGDLVGVRLRGDSTPVPLVATPFTELHPALSPD
                     GRWLAYTSNESGINEVYVRPFLNPAGGRWQISNGGGSEPRWSPKGNELFFLDRSPWLN
                     AAQVQTAPTFAVSEVRPLFDASQFSSDPFHQSYDVTPDGSAFIFGSPRQQGARASGFR
                     LVWVDNWFSDLQARVKQ"
BASE COUNT          678 a         1246 c         1285 g          699 t
ORIGIN      
        1 tcctcgctga ttccgtcgct gaagtactca ttggcactgt ccccgctcaa attcgtaaag
       61 ggaagaacag ccacagagcg agccgcatcg accgcgggtg ccacgacatg tgccggctgt
      121 cgcttgcgag taatcagaat ccccagcgcg gctgctacaa ccagcacgcc aactaccgcg
      181 agcgtcttcc atcgccggga gcgtctcgcg ggctcgctgg ggaggctggg cagcgcagct
      241 gttaccccgc tgggcgtcgt cgctgcctcg agtgtgggga aaagctgctc ggcgctttga
      301 ggccggtcgg ccgggtgctt ggcgagaagg cgcatcacca ggtcggccag agatggcgga
      361 aggccggctc gatgaccgag tatcggcggc ggcggcgtgg ttacctgagc cgcgaggatc
      421 tgttgcgggg ttcccccggt gaagggcggt tgtccggtaa gcatctcgta ggcaacgacg
      481 ccgagtgcat acaggtccgc ccgatggtcc acgtggggat cgccggccgc ctgctccggg
      541 gacatgtagg ccggcgttcc gacagccata cctgcgctgg tgaggttccc cttgctcccc
      601 gcttcgctga ccgccttggc tacaccgaaa tcggcgacca gggcatgacc gtcggagagg
      661 agaatgttct ccggcttcac gtcccgatgg acgatgccgg cccgatgggc cttgccgagc
      721 gcatcggcga cctcgtggag caaccgggcc gcctcgggaa cgggaagcgc gccttcccgc
      781 gtcatttttt gccggagaga ctctccagcg acgtacggca tcacataata gagaagacca
      841 tcggcctggc ccgagtcata taccgggagg atgtgggaat gagtgagccc ggccgcgatc
      901 tctacttccc gcaggaaccg ctccgcggcg agagcctcgg agatccccgg gcggagtacc
      961 ttgatggcta cgcggcggcg gtgtttgaga tcctcggcga gataaacagt ggccatcccg
     1021 cccgcaccga gctcgcgttc gatgcggtag cggtcggcga gcgcggcagt gagcttcttc
     1081 tctataggca cgcccgaaga tacggtaaac gggcggagta gcggaagcgg aggtccaatc
     1141 tcatggggga atacaagcat gccggtgttt gacacggtca ggcccggctg gcacttcggc
     1201 acccgcaaga agacctcgcg gctgggccga tttattctga gcgagaacca atgcccgccg
     1261 aacctgcgga ctccgtggca cggtcacgag actgcggcgt tctgcctggt gctcaggggt
     1321 cgctacctcc agcgattccg gcaccgcgac gtggtatatc agccttcagt agctcttttt
     1381 cgaccgcccg gtatcgagca caccgatcag gtctcacccg agggcgtggc gtccttcttt
     1441 gtggagcccg acccggcgtg gctggcccag atgggcctcg agcagttgaa tgtcgactac
     1501 gcgctcgccc ggtccggacc aagaggacgc tggctgctgg aacagatctt ctccgaattc
     1561 agctcgcccg acagggcgag cccgctcgcc ctcgaggggc tggttctcgc actcggcgcc
     1621 gagttcgctc gccagcccga agcccggatc cggcgaccgc cggggtggct cgagcgaacc
     1681 cgtgaggcgc tggacggaag ccttgcggaa cggctcacgc ttgcggcgct ggccgccgaa
     1741 gccggagtgc atccggttca tctcgccgcg gccttccggg ctgcgtacgg tacgtcggtg
     1801 ggtcaatacc tccgtgcccg gcggatcgat gcggctcgct cggaactgct cgatcacagg
     1861 cgctccatca acgagatcgc actcagcttt ggcttttcca gccagtcgca tttcacccgg
     1921 gtgttccggg agcactctgg gatgacgccg cgggcgtacc gtcggctgca tcgtgtgatg
     1981 cctgagatcg aggttcggcg cgcgtagtcg agcttcggct cgtgccagat ccctttggtt
     2041 agtgatagat cgtcgagctg ctccggtcta tcgttcctgc atgcgccgcg tggttcagct
     2101 tccactggcc gtcgccctct ccgtcgtcgt gtgctcgtcg gcagtcgccc agagcgcaat
     2161 ctcggccctg ccccggagca aggtccagcg ggcagatgcg gtgatcgcag actacatgac
     2221 ggcgcaccag gtgcccggac tttcggtcgc gattggtctc gaccagcggt tgatctggtc
     2281 gaaggcctac gggctcgccg acatggaaga ctccatcgcc gcgaccaccc gttcaatgtt
     2341 ccgtacggcg tcgatcggaa agccgatgac ggcgacggcc gtcatgctgc tgtggcagga
     2401 gggcaaactc gatctcgacg ccccgatcca gagatactgt cgcgcgtttc ccgagaagcc
     2461 gtggccagtg actgcccgcc agctgctggc gcaccgaagt ggaattcgag acaagacgcc
     2521 ccaggaggac agcaactaca cgcattatgc tacgatcgaa gcgtcagtcg ccatcttcgg
     2581 cggcgactcg ctgctgttcc agccgggcac ggctttccgc tatagctcct acaactacga
     2641 cgtgctgggc tgcgcgatcg aaggcgcctc cgggatgtcg tacatggact acatgaaccg
     2701 caggatcttc ggaccggtgg gcatgtcaca cacgctgctc gatgatccga aaccgatcat
     2761 tccccaccgc gtggccggat acgcgctcga taagcagggc aagctcatca attcgatcca
     2821 tgacgacatg agcaaccgga ttccggccgg cgggttcgtc tccactccga gcgacctggt
     2881 gcgcttcggg ctcgcggtac tggccgggaa gctggtggcc gacagcaccc tccagttcat
     2941 gtggcaggtc cagcgcgcgc cgacggattc agtcaccaaa gagggctacg gactcggctg
     3001 ggcactgggg gactggtttg gagtgcagga ggtctcccac ggcggcggca cgccgcaggt
     3061 ctgcgcgctg ctctacctgc tgccggccaa gcgcatcgtc gtcgcgttca tgatgaacct
     3121 ggagacggta ccggatcggg gggacctggc cggcgatctg gtgaaggttg tgctgggacc
     3181 caacgcgccg cacaactgag ctactgcttc acgcgagcct gcaggtcgct gaaccagttg
     3241 tccacccaga ccagcctgaa accgctcgcc cttgctccct gttggcgcgg gctgccgaag
     3301 atgaaagcac ttccgtccgg agtgacatcg taggattgat gaaaaggatc gctcgagaac
     3361 tgacttgcgt cgaacagggg tcggacctca ctcacggcaa acgtgggcgc cgtctgcacc
     3421 tgtgccgcgt tgagccaggg cgatcgatcg aggaagaaaa gctcgttgcc cttgggggac
     3481 catctcggct cggacccgcc tccatttgag atctgccagc ggccaccggc cgggttgaga
     3541 aatgggcgga cgtaaacctc gttgattccc gactcgttcg aggtgtaggc cagccagcga
     3601 ccatcgggcg agagtgccgg gtgtagctcg gtgaagggtg tcgccaccag cggtaccggc
     3661 gtcgaatccc ctcttagccg cacgcccacc aggtcgccgg cgccgggcgt ggcgttgtca
     3721 gtacgcagga tgagccattg gccgtcgtgg gaccagagag cctcctgaat ctgacggtcg
     3781 agacgagcca cgagccggtc ctcaccgctg ccgtcagcgg gacgagcata cagcatcgtg
     3841 gtcgccccgc tgtcgcgcag gaaagccacc gtccggccgt cgggcgacca gatcggtcgg
     3901 cgatcagc
//
LOCUS       DASRJQ010000101         9039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_495935, whole genome shotgun sequence.
ACCESSION   DASRJQ010000101 DASRJQ010000000
VERSION     DASRJQ010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 9039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9039
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495935"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(478..771)
                     /locus_tag="VFB89_02305"
     CDS             complement(478..771)
                     /locus_tag="VFB89_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26164.1"
                     /translation="MSGAAGLDVRWPIGGLFAVLGLILAGYGIATAGHMDLYARSLSI
                     NVNLWWGLVLLVFGAALLLGARAGRGRAGVKQALDTAEGRQTEAREHQLGLER"
     gene            complement(780..2534)
                     /locus_tag="VFB89_02310"
     CDS             complement(780..2534)
                     /locus_tag="VFB89_02310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HZM26165.1"
                     /translation="MAAAPVVQLTPVDYAVMATYFIAVLGVGWGLRRVMRTSTDFFLS
                     GRSLSPWITGLAFISANLGAQEVIGMGASGAKYGIATSHFYWVGAIPAMVFVGLFMMP
                     FYYGSRARSVPEYLRLRFDEKTRTLNAASFAVMTIFSSGVSMYAMGKLMNLLLGWSFD
                     LSIVVSACIVLAYVLLGGLTSAIYNEVLQFFLIVFGFAPLVGLGLVAVGGWQGLSTRL
                     SEVATMRGLPPDAYTHAWTNLDSAAHNPVGVEWFGLVAGLGFVLSFGYWCTDFLVVQR
                     AMAADSMTAARRTPLIAAVPKMLFPFLVILPGMIALALGSGGSGQGLVPAKLAADGTP
                     LLDAAGRAVLDYDLATPMMLVHLFPSGLLGLGVTALLASFMSGMAGNVTAFNTVWTYD
                     IYQSHIRRGASDAHYLWMGRIATVGGVFLALGAAYVAGAFNNIMDLLQLVFAFVNAPL
                     FATFALGMFWRRSTGHGAFWGLLAGTLAAAVHHGLTLPAGTHVGVKGGYLGVVHTYPS
                     ELAQTFWTAIVAWVTCFLVTILVSLATAPRPAEELRGLVYSLTDRPADESLPWLRRPS
                     TLAALVLVLTLALNLVFF"
     gene            complement(2597..3295)
                     /locus_tag="VFB89_02315"
     CDS             complement(2597..3295)
                     /locus_tag="VFB89_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26166.1"
                     /translation="MTAPSQMSLRRIPDALCLRVRIAYATAWDALIDTHTTQALQFVC
                     EFAPRLPVLGALDLYFRVTAVPEAMQEAVRVGTLTVIDVESLPPLTVLPSLTGWQRLR
                     LDLLIEQLQYRRRYHERTLALARMVGARSAEAVVATHVENAIGFARLLKGIMPVNLAT
                     DRYIREFSLPASIAEMVMQRVQARVAGEELTAQYNDPPPPTELDELVEQPAPRLESPA
                     NSASPFPEPAARGF"
     gene            complement(3652..4152)
                     /locus_tag="VFB89_02320"
     CDS             complement(3652..4152)
                     /locus_tag="VFB89_02320"
                     /inference="COORDINATES: protein motif:HMM:NF018925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HZM26167.1"
                     /translation="MRRNSIAVAALKGAIGGALATWMMDRVTTWAYERESEPVRKRED
                     EARQGKTAYEVAAAKVARRVGHTRLESEAQGRYGNVIHWTLGIGAGALYGALRKRAPI
                     VGVGRGVGFGILFWLLVDELATPVLGLSPGPRAFPWQTHARGLGGHLAYAAALTTALD
                     AVEAAG"
     gene            4367..4855
                     /locus_tag="VFB89_02325"
     CDS             4367..4855
                     /locus_tag="VFB89_02325"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HZM26168.1"
                     /translation="MESRVKFAGHSVHQMMIVFPLGLLGSAAIFDIIYLVSENSDWTQ
                     AAFYMIIVGCVSGLLAAVPGLLDWLVIPRGTRAKRIGLWHALGNVLVIGLFCASWILR
                     YPDPATPPLRSLGLGFIGVGIAIVTGWLGGELVDRLGVGVDDGAHLNAPSSLTRHPAQ
                     GR"
     gene            4859..5014
                     /locus_tag="VFB89_02330"
     CDS             4859..5014
                     /locus_tag="VFB89_02330"
                     /inference="COORDINATES: protein motif:HMM:NF039824.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HZM26169.1"
                     /translation="MFLWIGILLAVVWVLGYLAFHVASGLIHLLILLAVISFLIHLMR
                     GSSRTIW"
     gene            5979..6377
                     /locus_tag="VFB89_02335"
     CDS             5979..6377
                     /locus_tag="VFB89_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26170.1"
                     /translation="MFSVNKALLVAVAGLIAGAVATPALAQQATSVTGCLSKGTGKDS
                     YTITGSDGKSYSLTSTSVKLDQHVGHKVTVTGTSAGMETGALKDTTMAKMDTAMKMDT
                     TKGAGMKKETGTGGGTLKVSDLKMVSAECK"
     gene            complement(6455..6808)
                     /locus_tag="VFB89_02340"
     CDS             complement(6455..6808)
                     /locus_tag="VFB89_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HZM26171.1"
                     /translation="MRLLLVLAVCSIPMLAPAQAAAQQLRPKVSPESAQSAALARVPN
                     GSVQAMQLKVERGTMVYIYDISAPGGSGLEEVQVSAVDGRVVSVQHLGGAPGRAATPG
                     QRVADRVNSPAPPRR"
     gene            6975..7577
                     /locus_tag="VFB89_02345"
     CDS             6975..7577
                     /locus_tag="VFB89_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26172.1"
                     /translation="MANIPLLVLLSSLTLPAATAAQLPDSIPPEPAPNSELPALASWI
                     ARWLPVVAVARYSAVDSTADFYAHGSDSVASARLDGCTLTLHEILVSTVRGKRSETRL
                     AIQVPLDQVDTTLIQPKIRQARLLLNRPNVLMYGQLVVPLRNRTRTEFITVFGEGDSR
                     YPILVNEHQVPFVFAQLPAARSALALRQAAARCEAAADLQ"
     gene            complement(7592..8374)
                     /locus_tag="VFB89_02350"
     CDS             complement(7592..8374)
                     /locus_tag="VFB89_02350"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZM26173.1"
                     /translation="MTQTNFRGRFVWHELLTSDPKAAATFYTRALGWKAEPWDEDPSY
                     TLFRSENGLVAGAMTLPAEAKAAGAKPHWLSYIGTPDLKGTVDSATRLGGKVLKDITP
                     IPTVGRFAIIADPQGAVFAAFTPESAPPDKPPATDFAWHELITTNHVAALRFYQELFG
                     WEKTDAMEMPRLGTYQIFGLNGVGMGGTYNKADDMPFPPHWLPYASVSDSPTVTRAVT
                     NLGGKVMVGPTEVPGGGLISVCEDPQGVMFAMHSVATAAAHA"
     gene            complement(8609..>9039)
                     /locus_tag="VFB89_02355"
     CDS             complement(8609..>9039)
                     /locus_tag="VFB89_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26174.1"
                     /translation="PEQVLAARPELRSLWYDEPTRQYGRPAAYYQAVQRLDVEAAWGA
                     LAERGIPALVVWGEFDWIMGRAEAERAVEIVNARKPGLAQLVVLAKTDHSLMAYASIA
                     AAFTDEGPRNDGGAGRAITAWLRERAAHADRTSAPGSRGR"
BASE COUNT         1708 a         2898 c         2915 g         1518 t
ORIGIN      
        1 gaggggagaa caaatggtcg aatccaacca gcgtgtgagc tcataatcgt cgcctccatt
       61 cccggaggcc gaatcctcag caggagcccg ggcgcgctcg cgaccctggg actacggcgg
      121 agtaatcggg ggcaacggga aaaagggatc ggcggtgtgt cggagcgtgg cagtgggcga
      181 ggagcgcgct gcaagggaaa cgcggcggag ggtccggttt cgtgcgggga gcacgagcct
      241 gctggagcgc agccgggatg gcatctcgat aacgcgaatg cgttggagca tcacgccgat
      301 cggttgtttg cacagcgcgg aggcactggg gctattgcaa cggcctccga gaacgggcag
      361 cggcaaggga ggttccgccg ctgacatcaa gccgatcaca cacgcatcgg cggcgcgata
      421 gtagtgacta gtgttgtaga gatgccgtgg cctgaatcac aggaggattg gtcgcgatta
      481 ccgctcgagg cctagctggt gctctcgagc ttcggtttgc cggccttcgg cagtatccaa
      541 cgcttgcttg acacccgccc gtccacgtcc agccctcgca cccagaagca atgccgctcc
      601 gaacaccagc agaacgagac cccaccaaag gttgacgttg atcgagagag aacgcgcgta
      661 caggtccatg tggccggctg tggcgattcc gtaacccgcc aggatcagcc cgagcacagc
      721 gaacagcccg ccgatgggcc agcgcacgtc caatcctgca gcaccgctca tcgtccctcc
      781 tagaaaaaca ccaggttcag cgcgagcgtc agcaccagga ccagcgccgc cagcgtagac
      841 ggacgacgaa gccatgggag tgactcgtcc gccgggcggt cggtcagcga gtagacgagc
      901 ccgcgcagtt cctccgccgg ccgtggcgcg gtagcgaggc tcacgaggat ggtcaccagg
      961 aaacaggtca cccacgccac aatcgcggtc cagaacgtct gggctaattc gctggggtag
     1021 gtgtgcacga cccccaagta cccgcctttc accccgacat gggtgcctgc cggcagggtg
     1081 aggccatggt gaaccgccgc ggcgagcgtc ccggccaaca atccccagaa cgccccatga
     1141 ccggtcgaac gtcgccagaa catgccaagc gcgaaggtgg caaacagggg cgcattcacg
     1201 aaggcgaaaa ccagctggag caggtccatg atgttgttga acgcgcccgc aacgtacgcg
     1261 gctccgagtg ccaagaagac gccccccaca gtcgcgatcc gccccatcca caggtaatgc
     1321 gcgtccgagg ccccccgccg gatgtgactc tgatagatgt cgtaggtcca caccgtattg
     1381 aacgcggtca cgttgccggc cataccggac atgaatgatg cgagcagggc ggtaaccccg
     1441 agccccagca agcccgaagg gaagaggtgg accagcatca tcggcgtggc gagatcgtaa
     1501 tcgagcacgg cgcgaccagc cgcgtcgagg agcggcgtgc cgtcggcggc tagcttggcg
     1561 ggaacaaggc cctgtcccga gccgcccgat cccagggcga gtgcgatcat gcccggcagg
     1621 atcacgagaa acggaaacag catcttgggc accgcagcaa tcagcggcgt ccgccgggcg
     1681 gccgtcatgg aatcggccgc catcgcgcgc tgaaccacga gaaagtcagt gcaccagtag
     1741 ccgaacgaca gtacgaagcc gagcccggcc accagcccga accactccac gccgaccgga
     1801 ttgtgcgccg ccgaatcgag attggtccac gcgtgggtgt acgcatcggg cggaagcccc
     1861 cgcatcgtcg cgacctcgga gagccgtgtg ctgaggccct gccatccccc gacagcaacg
     1921 agtcctaagc ctaccagcgg ggcaaagccg aaaacgatga ggaaaaactg aagcacctcg
     1981 ttgtagatgg cactggtgag cccgcccagc agcacgtagg ccaggacgat gcaggccgag
     2041 acaacgatgc tcaggtcgaa gctccagccg agcagcagat tcatgagctt ccccatggca
     2101 tacatcgaca cgcccgagga gaagatcgtc atcacagcga aggaggcggc gttcaacgtc
     2161 cgggtcttct cgtcgaaacg cagtcgcaaa tactccggga ccgaccgggc ccgcgagccg
     2221 tagtaaaacg gcatcatgaa caggcccacg aagaccatcg ccggaatggc gccgacccag
     2281 tagaagtggc tggtcgcgat accgtacttg gcgcccgacg cgcccatgcc gatgacttcc
     2341 tgcgcgccga ggttggctga gatgaacgcc agcccggtaa tccagggaga aagcgaacgc
     2401 ccggagagga agaaatcagt gctggttcgc atcactcgtc gcaggcccca gccaaccccg
     2461 aggacggcga tgaagtaggt cgccatcacg gcatagtcga ccggggtgag ctggacgacg
     2521 ggtgcggcag ccatggcaca caaggtagcg ggagacgtcc cccgccgcag gcgctgctat
     2581 gatcgcggat gactcgtcag aagccgcggg cggcaggctc cggaaatgga gatgcagaat
     2641 tggcgggaga ttcgagccgg ggtgccggct gctcgacgag ctcatccagc tcggtcggtg
     2701 gcggcgggtc attgtactgg gcggtcagct cttcgccggc tacgcgcgcc tgcactcgct
     2761 gcatcaccat ctcggcgatg ctcgccggca gactgaactc gcggatgtag cgatcagtgg
     2821 caaggttgac gggcatgatg cctttgagca gccgcgcaaa accgatggcg ttctcgacgt
     2881 gggtcgccac cactgcttcg gccgagcgcg cgccgaccat ccgggccagc gccagcgtcc
     2941 gctcgtgata ccgacgccga tattgcagtt gctcgatcaa caggtccagc cggagtcgct
     3001 gccagcccgt caatgacggc aggacggtga gaggcggcag tgactcgacg tcgatcaccg
     3061 tgagagttcc cacgcggaca gcttcctgca ttgcttcggg aactgcggtg accctgaagt
     3121 agaggtcgag ggcgccgagg acgggtagcc gcggggcgaa ctcgcagacg aactggagcg
     3181 cctgggtcgt atgggtgtcg atcagcgcgt cccaggccgt cgcgtaggcg atgcggaccc
     3241 tcaggcacag cgcatcggga attcgacgca gcgacatctg gctcggcgcc gtcatgctac
     3301 cactccgggt gtgttgcgga aggcgcgcgc ggccgcacgc gccggtcgga cctgatgcgt
     3361 cagagcaata tccagtccct gtctggttgg tgcggccggc cggggctggt atcctggcac
     3421 gcttccgtcg ttgccagctt agccattgca cgccaacaag ttatgcgatg tccgccttag
     3481 ctgcggccga tcgcccggtc gggggcacga aggggcgcga gagagcacga cgcatcgcaa
     3541 tccgcacggg gagtcgcaga gaccccagcg cctgggcgag gcaccgcttc atgccgcggt
     3601 aagcatggcg ccctgaacgg aaatcacgat cggtttgttg cggcgccgga cctatcccgc
     3661 ggcctcgact gcatccagcg cagtggtcag agctgccgcg tacgccagat gccctcctaa
     3721 tccgcgtgca tgtgtctgcc agggaaaggc gcgaggccct ggcgacagtc ccagcaccgg
     3781 agtcgccaac tcatccacga gcagccagaa caggatccca aacccaacgc ctcggcccac
     3841 tcccacgatt ggcgcgcgct tgcgcagtgc accgtagagc gcaccggccc cgatgccgag
     3901 agtccagtgg atcacgttgc cgtagcggcc ctgagcctcc gactcgaggc gggtgtgtcc
     3961 cacgcggcgg gcgaccttgg ccgctgccac ttcataagcc gtcttgccct gtctggcttc
     4021 gtcttcacgc ttccggaccg gctcgctttc gcgctcatac gcccaagtcg tcacccgatc
     4081 catcatccag gtagctagcg cccctccaat tgcccccttg agagctgcta ccgcaatgct
     4141 gtttcgccgc atgataagaa cacctccgcc ttgcccgagt tgatcctggg gttgaccccg
     4201 aaccagaatg ccacggccct gaagcaaatg agagtgacga caatcactcg caggctccag
     4261 cgagttgttt caggacggcg cgcggaaccg actggagggt gcgcgtcatc accgacgtcc
     4321 ggttaagcgg cccccatcgt gcgctcaccc tctagtggag ctcaccgtgg aaagcagggt
     4381 caagttcgcg ggccactcgg tccatcagat gatgatcgtg tttccgctgg gcttgctcgg
     4441 atcggccgcg atcttcgaca tcatctatct cgtttctgaa aattccgact ggacccaggc
     4501 ggcgttctac atgatcatcg tcggctgcgt gagcggcctg ctcgccgcgg taccgggcct
     4561 gctcgactgg ctggtcattc ctcgtggcac gcgggccaag cgaatcggac tctggcacgc
     4621 acttgggaac gtgctcgtga tcggcctgtt ctgcgccagt tggatcctgc gttatcccga
     4681 tccggccacg ccgccactcc ggtcgctggg gcttggcttc atcggtgtcg ggattgccat
     4741 cgtcacaggc tggctgggcg gggagttggt cgatcgtttg ggggttggag tcgatgatgg
     4801 cgcacatctg aatgcgccga gttcgctaac gcgtcatccg gcgcaaggac ggtaactcat
     4861 gtttctctgg attgggatcc ttctggcagt ggtttgggtc ttggggtatt tggcattcca
     4921 tgtcgcgagc ggcctgatcc acctgctgat cctgctcgct gtcatctcgt tcctcatcca
     4981 tctcatgagg gggagcagcc gaaccatttg gtgaggccgg gagtcgagga gaggccgatc
     5041 accggaacca ggtcaggatc gccgggcagc gctggccggc tcctcctatc agccagcccg
     5101 caaccctctt ccaggcctgc ctttaccgga gcttgaacgc ttggtcacgc ttgcaggggt
     5161 aagccccttc cagggtcgga ggcggtgagt gaaatcacct taccgagacc ctagcagtcc
     5221 ggcggaacgg cccgtaggtt agcggaggaa gtcctcgctc gagacgtaca ggagtcgttt
     5281 tgctatgtcc ttcccatcgc ttttcccgcg gggcaattca ctcactgaat ccatgccctt
     5341 cgcggaccga gaaggtgtca cctggctggc gtacatcgag ggcgtcgccc cccccgacca
     5401 tcaccatgcg gtgaacggtc gtctgcccgg acgacggctt cgtttcgatt cggaggatga
     5461 gtcacggaca actaccgagc ttcctgccgg ggcaccgttt ctttccgacg aacgattgca
     5521 ggctctgctg gaccgggctc agcccgttac agcaccgacc accggcagct ggcagcttcc
     5581 tgagccgccc atcaaactcc aacgggcgat cgagtggacc gcgcacgccg gccgcgcagg
     5641 cgcggccctg ttggtggaat tggttcggcg ctggcgtgaa ggcgcggatc gccgacgtga
     5701 gttgcgcgag caggcagcgg acctcatgtc tgcagcctac gaaagagccc gccagttagc
     5761 cgctcaagta aggcaggaaa tcgaggagca gcacggcagc agcccaccac ctccggcgga
     5821 accaaccgtc ccgcgttcag tcataggtcg gcggtggcgg ggggcgcggt cggcggggag
     5881 actgggcccc cgataacaga aacggtcgcg gcggcggaag cgcaccccgc ctcgaccaga
     5941 cgtgacgatc tcaataccgg tgcgcggagg agacataaat gttcagcgtc aacaaggccc
     6001 tgctcgtagc agtggcgggc ctcatagcgg gagccgtggc gactcccgct ctagcacagc
     6061 aggccaccag cgtgaccggc tgcctgtcaa aggggaccgg taaggacagc tacacgatca
     6121 cggggagtga cggaaagagc tattccctga ccagcacctc agtcaaactc gaccagcatg
     6181 ttggccacaa ggtgacggtg accggcacgt ccgccggcat ggagaccggt gcgctcaagg
     6241 ataccaccat ggcgaagatg gataccgcca tgaaaatgga taccaccaag ggcgcaggca
     6301 tgaagaagga gaccgggacg gggggtggca cgctcaaggt cagcgatctc aagatggtca
     6361 gcgccgagtg taagtagcgc cgagccacga atgagaaccg ccgcccctga ggggcggcgg
     6421 ttctggttcg tgggacatgg atcaagggtt cgagtcagcg tcgcggagga gcggggctgt
     6481 tgacgcggtc cgccactcgt tgtcccggcg tcgctgcccg gccaggagcg ccaccgagat
     6541 gctggaccga cacgacccgc ccatctacgg cgctcacctg aacctcctcg agaccgcttc
     6601 ctccgggcgc cgagatgtca tagatgtaca ccatggtgcc gcgttcaact ttcaactgca
     6661 tcgcctggac gctgccgttg ggaactcggg cgagggcagc cgactgtgcc gactccgggc
     6721 taaccttcgg gcggagttgc tgcgccgcgg cttgagcggg cgccagcatg gggatcgagc
     6781 agacggctag aacgagcaag aggcgcatac cgggctcctt ggcagtgacc gattcagcct
     6841 agccgtttct ggtgggcccg acggtaggct gacgcacagc ggcggcgtga gccaaacgag
     6901 tcgcggttta tctagccgct gtgcttggat ttaccttagc atgtcaatca ggcgccgcat
     6961 tttgcctcgg ttcaatggcg aacatcccgc tactcgtcct gctttcgtcg ctcacacttc
     7021 cggcggcgac cgcggcgcaa ctccccgata gcattccccc ggagcccgca cccaactccg
     7081 agttgcctgc acttgcgtcg tggattgcac gctggcttcc cgttgtcgcg gtcgcgcgct
     7141 actccgccgt ggacagcacc gcggacttct acgctcacgg gtccgactcg gtcgcctcag
     7201 cccgactcga cgggtgcaca ctcacgctgc acgagattct ggtcagtacg gtgaggggga
     7261 aacggtcgga gacgcggttg gcgattcagg tgcctctcga ccaggtcgat acgacgctga
     7321 ttcagcccaa gatccgccag gcgcgactgc tgctcaatcg ccccaatgtg ctgatgtacg
     7381 gacagctcgt ggtgccgctg cgcaatcgca cgcggaccga gttcattacg gtgtttggcg
     7441 agggtgattc gcgatatccg attctggtaa acgagcacca ggtgccgttc gtgttcgcgc
     7501 agctgcctgc ggcgcgatcg gcgctggcat tacggcaggc cgcggctcgc tgtgaagcgg
     7561 ccgctgacct gcagtaagcg ttgccaggcg atcaggcgtg ggctgctgcc gtggccaccg
     7621 aatgcatggc gaacatgacg ccttgcgggt cttcgcaaac cgagatcaga ccgccacccg
     7681 gcacttccgt cggtccgacc atcactttcc caccgaggtt cgtcacggcc ctggtcacgg
     7741 tgggggagtc ggaaaccgag gcgtatggga gccagtgcgg cgggaaaggc atgtcgtcgg
     7801 ccttgttgta cgtgccgccc atgccgacgc cgttcagccc gaagatctgg tacgtgccga
     7861 gccgcggcat ctccatcgca tccgtcttct cccacccgaa cagctcctgg tagaaccgaa
     7921 gcgctgcgac gtggttcgtg gttatcagct cgtgccaggc aaaatcggtc gcgggtggct
     7981 tgtcaggagg tgccgactca ggcgtgaacg ccgcaaagac ggcgccttgt ggatccgcga
     8041 ttatcgcaaa acggccaact gtcggaatgg gagtgatgtc cttcagaact ttcccgccca
     8101 accgagtggc cgagtccaca gtgcccttga gatccggagt tccgatgtag gacagccaat
     8161 gcggcttggc tcccgcagcc ttcgcctcgg cgggaagggt catggcacct gccacgagac
     8221 cattctcgct cctgaacagc gtgtatgagg ggtcctcgtc ccacggctcg gccttccagc
     8281 cgagcgcgcg cgtgtagaag gtcgctgcgg ccttgggatc gctcgtgagc agctcgtgcc
     8341 acacgaagcg tccgcggaaa ttcgtttgcg tcatcgatgt cctcctctaa atgaatcccc
     8401 agcgccggga ggccggcgcc gacggaccgc caacctacgt cgggcccctc atcggcgcca
     8461 tgactattcc tgctaccagt tggccgaatg ggccccaagg ccgcttgcct aaggtcggtc
     8521 gacgatgaat gtcacagggg gctagcggga gtcagtggtt gtttgcgcca ctaacggaag
     8581 ccgacgccga cggtgatcga gacctcgctc atcgcccccg gctgcctggt gccgatgtgc
     8641 gatcggcatg ggcggcccgc tctctgagcc acgccgtaat tgcccgcccg gcccccccgt
     8701 cgttcctggg accctcatca gtaaatgcgg ccgcgatgct ggcgtaggcc atgaggctgt
     8761 gatcggtctt cgcgagaacg acaagctgcg cgagtccagg cttgcgcgcg ttcacgatct
     8821 cgactgccct ctcggcctcc gcacgcccca tgatccaatc gaattcgccc caaaccacca
     8881 gcgcgggaat gcctcgctcc gccagcgcgc cccaggcggc ctcgacatcg agccgctgca
     8941 cagcctggta gtacgccgcc gggcgaccgt actggcgggt gggttcgtcg taccagaggg
     9001 aacgaagctc aggccgagcg gcgagcacct gctccggtg
//
LOCUS       DASRJQ010000102         2709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1058376, whole genome shotgun sequence.
ACCESSION   DASRJQ010000102 DASRJQ010000000
VERSION     DASRJQ010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058376"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="VFB89_02360"
     CDS             <1..1038
                     /locus_tag="VFB89_02360"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HZM26175.1"
                     /translation="TNAAASNWPLFIPVAVLVGMVLLWRKVGRDPEGLPVAVQYEPPG
                     EMTPAEVGTLLDESVDMRDITATIVDLAVGGHLRIEEREEKTLLGLWSRTEFVFHQTQ
                     PPDGTRKLTSHEQRVLGGIFSGRAGEVRLSDLKNEFYSELPGIRTAVYECLVDRGLHR
                     SRPDKVKKGWTMAGVVTGAVLAFGGGGQFAARMGLTPVPFVLAGIVSALIILLFGRIM
                     PARTIAGARMLEKVRGFEEFLTRVESDRFARVVKTPEMFEKFLPFAIAFGVEAKWARA
                     FRNIYTEPPRWYAGTNAVAFNAGSFSSRLSEMSEQAGRTMSSSPRSSGGSGFSGGSSG
                     GGSGGGGGGGF"
     gene            1370..1789
                     /locus_tag="VFB89_02365"
     CDS             1370..1789
                     /locus_tag="VFB89_02365"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HZM26176.1"
                     /translation="MRDSIVMVARSQIGTRYKYGGTTPTGGFDCSGFVRYVLNAIQLV
                     LPRTAAQQAELGATIPKDTTRLRPGDLLTFGRGGRVTHIGIYVGNGRYVHASPTAGRV
                     IETSLERTESPLVKIWRGGRRLLAERDWLSDTTAAAP"
     gene            2071..>2709
                     /locus_tag="VFB89_02370"
     CDS             2071..>2709
                     /locus_tag="VFB89_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26177.1"
                     /translation="MAMDIKREPPKKRGKYILIGVGVAVLGITTVALGNLEQRAPSVD
                     RATLWIDSVQRGTMTRQVRAPGTLTPEHIRYVVAVTSGRVEVLPVRPGVTVTPSTLLL
                     ELSNPDVQLEMLNAQSAVTSARQNYVTLQTTLESQRLQLETQVANARTQYGNAKRDFE
                     TFQALAAKTPPLASPNEVARATEAMDEGDKRLRSLEQQLKVQTDAISEQLRLA"
BASE COUNT          483 a          832 c          893 g          501 t
ORIGIN      
        1 acgaacgccg ccgcgagcaa ttggccgttg ttcattcccg ttgctgtgct ggtcggcatg
       61 gttctgcttt ggcgcaaggt tggccgcgac cccgaagggc tcccggtcgc cgtccagtac
      121 gagcccccgg gcgagatgac cccggctgag gtgggcaccc tgctggatga gtcggtcgac
      181 atgcgagaca tcaccgcgac gattgtcgat cttgcggtcg gtggtcacct gcggatcgag
      241 gagcgggagg agaagacgct cctcggcctc tggtcgcgga ccgagttcgt ttttcaccag
      301 actcagccac cagacggtac ccgcaaactc acctcacacg agcagcgggt gctgggcgga
      361 atcttcagcg ggagagcggg tgaagtccgc ctctccgatc tcaagaacga gttttatagc
      421 gagttgcccg gcatacgaac cgcggtctat gagtgtcttg tcgaccgtgg gctgcatcgg
      481 tcccgccccg acaaggtcaa gaagggctgg acaatggctg gtgtcgttac cggtgcggtc
      541 ctggcattcg gcgggggcgg gcagtttgcc gcacgaatgg gtctcacccc ggtgccattc
      601 gtgctggcgg gaatagtcag cgccctgatc atcctgctgt ttggccgcat tatgccggca
      661 cgaacgatcg ccggtgcccg gatgctggag aaggtgcgcg gcttcgagga attcctgacc
      721 cgcgtggaga gtgaccgctt cgcgcgagtg gtgaagacgc cggagatgtt cgagaagttt
      781 ctcccattcg cgatcgcctt cggtgtcgag gccaagtggg cccgagcctt tcggaacatc
      841 tacaccgagc cgcccaggtg gtacgctggt accaatgccg tggcattcaa cgccggcagc
      901 ttctccagcc gcttatcgga gatgtccgag caggctggaa gaacgatgag ttcttcgcca
      961 agaagctccg gtggctccgg gttcagcggt ggttcctccg gagggggttc gggtggggga
     1021 ggtgggggag ggttctaggc ccgggatgac gggcacatgc gctggccagg aagtcaacag
     1081 acgactctgc cgcatgccga tcggatctgg ttgtggcgag cggggcggca gtagctttcg
     1141 atccgcggcg cttggcaacg gcctcttacg tgcgtgtgtc aaacccaaga tcatgcctca
     1201 ctcgccacgc catccgtcgc cagtctcgcg tcgccttcgc catagcgcga tcgcggcgat
     1261 ggtggcgttt gccccgatag tggccgcggc acagaacact gaggacgcga acgagccagc
     1321 tccgcgggat cgccggccat tcgcggcttt gagcgccagc gcgctgtcaa tgcgcgactc
     1381 catcgtcatg gtggcgcgct cgcagatcgg aacccgctat aagtacggtg gcactacgcc
     1441 caccggcgga ttcgactgca gcggtttcgt gcgctatgtc ctgaacgcga tccagctcgt
     1501 cctgccacga accgccgctc aacaggccga gcttggcgcg acgatcccaa aggacacgac
     1561 tcggctgcgc cctggcgatc tgctcacgtt cggacgtggc gggcgggtga cccacatcgg
     1621 gatctacgtc gggaacggcc gatacgtgca cgcgagcccg accgcgggac gggtgatcga
     1681 gacctcgctg gagcggacgg agtcgccgct ggtgaagatc tggcggggag gcaggcggct
     1741 cctcgcggag cgggactggt tgtcggatac gaccgcagcc gcgccgtagt attaccgcct
     1801 aacgtcgccg acgcggggcg agcgctcgag ccggctacat tccgttgtag ccggctcgtt
     1861 agtttttccg gaacgtccgg ttttgggacg cgaggtccca tatctgggcg cgaacagcgt
     1921 atgcgccggc agaaccggca tcgtaacata ttgaaagtac gcatcttgcg atgcaggtac
     1981 gatggcacgg ctcttccctt tgcgactgtt tgaactgttc gtgcttttct cctgtcaaac
     2041 cgaacgattt taaagcaagg ggacgtcctc ttggcgatgg acatcaagcg ggaaccgcct
     2101 aaaaagcgcg gcaaatatat cctgatcggc gttggtgttg ccgtactcgg catcaccacg
     2161 gttgcgctcg gaaatctcga gcagcgcgca ccgtcggtcg atcgggcaac gctctggatc
     2221 gattccgtac agcgcggcac catgacccga caggtccgcg cgccgggtac cttgaccccc
     2281 gagcacattc ggtacgtcgt ggccgtgact tccggccgcg tcgaggtgct tcccgtgcgc
     2341 cctggcgtca ccgtcacgcc atcgacgctg ctgctcgagc tgagtaaccc ggacgtgcag
     2401 ctcgagatgc tcaacgctca gagcgcggtg acgagcgctc ggcagaacta cgtcacgcta
     2461 cagacgacgc tcgagtcgca gcgcctgcag ctcgaaacgc aggtcgcgaa cgcgcgaact
     2521 cagtacggca atgccaagcg tgacttcgaa accttccagg cgctcgccgc gaagactcct
     2581 ccgctggcgt cgcccaacga agttgcgcgg gctacggagg caatggacga gggcgacaag
     2641 cgattgaggt cgctcgagca gcagctcaag gtccagaccg acgccatctc cgaacagctc
     2701 cgtttggcg
//
LOCUS       DASRJQ010000103         3293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_814027, whole genome shotgun sequence.
ACCESSION   DASRJQ010000103 DASRJQ010000000
VERSION     DASRJQ010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814027"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VFB89_02375"
     CDS             complement(<1..639)
                     /locus_tag="VFB89_02375"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HZM26178.1"
                     /translation="MNDPVRFSVAGLVACLVLVMGCGNQETKSEDNTETPADSTVPSS
                     SASDKPLIAVVRAADWMGSEWSEDAIKLGLRESDMEPGRDYQLKLTSAQGDLTALPNL
                     IDAAVDAKAKVIVTLQDATLQVAVQRVKSIPIVFHLLADPFAAGAGKSDSDHLPNVTG
                     VYSPGFGDPEQTRRVELIRRIVPRARHIGVLFSPQEALSVTFKDRLTKAARDA"
     gene            complement(706..2970)
                     /locus_tag="VFB89_02380"
     CDS             complement(706..2970)
                     /locus_tag="VFB89_02380"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM26179.1"
                     /translation="MNVPELLERLSVVLSARYRIERELGQGGMAKVFLAHDLKYERMV
                     AIKVLRPDLSAEVGATRFLREIQIAARLHHPHILPLYDSDQAGDLVYYVMPYIEGETL
                     KSRLARERQLPVTDAIQIAREVADALSYAHNAKVVHRDIKPANILLDAGHALVADFGI
                     ARALGEAQTTSGHLIGTPAYMSPEQVDGSEHLDGRSDIYSLGCVLFEMLVGAPPFRGS
                     TVTAVIANRLSAPVPSPRAFREMIPEAVDAAVRKAMATLPADRFSTASQFSEALSTPV
                     AVAVAVGAAHAMAQDIAAVAKSVAVLPFENMSTDPENEYFSDGITDDIIAQLSKISAL
                     KVISRTSSMQYKKSTKKITAIAEELGVGAVLEGSVRKAGQRVRIVAHLVDPKTEKHLW
                     GDTFDRKLDDIFEVQSEVAQQITGALEVALSPEEKQRVEKKATGDADAYNLYLLGRFQ
                     ANKWSEADVQKGIEYYQQAVSKDPGYAVAYAGLADAYELLSIGFSSKPPVEYLATAKT
                     MALKALEMDDSLAEAHTSLAYARWLGDLDWPGAEREFKRALELKSSYVMAHEWYAEYL
                     AALGRHEEAIAEIKRAQQLDPLSVPVNRAVGWVLYFARRYDQSINELQKALAMNPEFL
                     GARLVLWWAWIAKGTPGDAIADLTREVDRPGLRTVKKLMLAYAHAAAGNREMADGILW
                     ELEPKLAGDNRMALLSAILFTAMDAKDRAFEQLERAYQVREPGLLFLKVAPWADELRS
                     DPRYGALVEKLVLG"
BASE COUNT          572 a         1071 c         1015 g          635 t
ORIGIN      
        1 ggcgtctcgc gctgcctttg tgagccggtc cttgaacgtg accgaaagcg cctcctgcgg
       61 actgaacaga acgccgatgt gacgcgcgcg gggcacgatt cggcggatca gttccacccg
      121 cctggtctgc tccgggtcgc cgaagccggg cgaatagacc ccagtgacgt tgggaagatg
      181 atcggagtcg cttttgccgg cgccggcggc gaacggatcc gccagcaggt gaaagacgat
      241 aggaatggac ttgacccgct gcacagcaac ctggagggtc gcgtcctgca acgtgacaat
      301 cacttttgct ttcgcatcga cggcagcgtc gatgaggttc ggcagcgccg tcaggtcacc
      361 ttgcgcgctg gtgagcttga gctggtaatc gcgacccggc tccatgtcgg attcccgaag
      421 gccgagcttg atcgcgtctt cgctccattc gctgcccatc cagtcggcag cacgaaccac
      481 ggcgatcagc ggtttgtcgc tcgcgctcga actcgggacg gtcgagtccg ccggagtctc
      541 ggtgttgtcc tcggacttgg tctcctgatt gccgcagccc ataaccagaa cgaggcacgc
      601 gacgagaccc gcgaccgaaa atcgaaccgg atcgttcatg ggtgagcctc ccattcggac
      661 tgacgacggc gcgggaggaa cgccgaacga agatgaggtt gccgcttagc cgagtacgag
      721 cttctccacc aacgcgccat atctcgggtc cgatcgaagc tcatcggccc agggggcgac
      781 cttgaggaac agaaggccgg gctcgcgaac ctgataggca cgctccagct gctcgaaggc
      841 ccggtccttc gcgtccatcg cggtaaacag gatggcggaa agcagcgcca tacgattgtc
      901 ccctgcgagt ttgggctcga gctcccagag gatcccgtcc gccatctccc gattgccagc
      961 tgcggcatgg gcgtatgcca acatgagctt tttcacggtt ctgagcccgg gacgatcgac
     1021 ctcccttgtg aggtcggcga ttgcgtcccc cggcgtaccc ttggcgatcc acgcccacca
     1081 gaggaccagg cgcgcgccga gaaattccgg gttcatcgcc agggccttct gcagctcgtt
     1141 tatggactgg tcataccgcc gagcgaaata gagcacccaa ccgactgccc ggttgaccgg
     1201 cactgacaac ggatcgagtt gctgcgcccg cttgatctcg gcaatcgcct cctcgtgccg
     1261 ccccagggcc gccagatact ccgcatacca ctcgtgagcc atgacgtagc tggacttgag
     1321 ctcgagcgca cgtttgaact cccgctcggc accaggccag tcgaggtcgc ccaaccagcg
     1381 ggcgtaggcc agcgaggtgt gggcctccgc caggctgtcg tccatctcca gcgccttgag
     1441 cgccatggtc ttggcggtgg ccagatactc caccggcggc ttcgagctga acccgatgct
     1501 gagcagctcg taggcatcgg ccagaccggc gtacgccaca gcatatcctg ggtccttcga
     1561 gactgcctgc tggtagtact cgatgccctt ctgcacgtcc gcttcggacc acttgttggc
     1621 ctggaagcgc cccaggagat agaggttgta ggcatccgcg tcgccggtcg ccttcttctc
     1681 gacccgctgc ttttcctcgg gggagagggc cacctccagc gcgccggtga tctgctgcgc
     1741 gacttcgctc tgcacctcga agatgtcgtc cagcttccgg tcgaatgtat cgccccagag
     1801 gtgcttttcc gtcttgggat cgaccaggtg ggcgacgatc cgaacccgct gtcccgcctt
     1861 gcgcacgctt ccctcgagca cggcgccgac gccaagctcc tccgcgatcg ctgtgatctt
     1921 cttggtggac ttcttgtact gcatggagga agtgcgggag atcaccttga gcgcgctgat
     1981 cttcgagagc tgcgcgatga tgtcgtcagt gatgccgtcg ctgaagtact cgttctccgg
     2041 atcggtgctc atgttttcga atggcagcac ggcgaccgac tttgcgaccg cggcaatgtc
     2101 ctgcgccatg gcgtgggcag cccccacggc gacggcgacc gccaccggcg tgctcaatgc
     2161 ctcgctgaac tgtgatgcag tgctgaatcg atcggccggg agcgtggcca tcgctttcct
     2221 gacggctgcg tcaaccgctt cgggaatcat ttcccggaat gcgcgaggcg agggaaccgg
     2281 cgcgctcagc cggttggcga tcacggccgt cacagtcgat ccgcggaacg ggggcgctcc
     2341 caccagcatc tcgaagagca cgcaacccag gctgtagata tcgctccggc catcgaggtg
     2401 ctcgctgcca tctacctgct ccgggctcat atacgccggc gtgccgatca agtgcccaga
     2461 ggtggtctgg gcctcgccga gagcgcgggc aataccgaag tccgccacca acgcgtgacc
     2521 ggcgtcgagc aggatgttgg cgggtttgat gtcgcgatgc acgactttcg cattgtgagc
     2581 gtagctcaac gcatccgcca cctcgcgcgc gatctggatc gcgtccgtca ccgggagctg
     2641 acgctcacgg gccagccgcg atttcagcgt ctcgccctcg atgtacggca tgacgtagta
     2701 gaccagatca ccggcctgat cggaatcgta cagcggcaga atgtgggggt ggtgcagtcg
     2761 ggcggcgatt tgaatttcgc ggaggaacct agttgcgccc acctctgcag agaggtccgg
     2821 gcgcagcact tttatggcga ccatgcgctc gtacttcagg tcgtgggcca ggaacacctt
     2881 cgccatcccg ccctgcccca attcccgctc gatcctgtag cgggcagaga gcactacact
     2941 gaggcgctcc agcaactccg gcacgttcac cgacctccgc tggttcccgg gaattaccgc
     3001 ctggacccga ctcgccgtat tggggacaca acagccgcgc ccccgccgcg acgcggtcag
     3061 ggcctcctgg cttcagcctg aggtttcaca tcggcggtct gttcccaagc agtcaatttt
     3121 actgcaagtt gagtcaatat agggattatc ccgatggtcg ccagattgct caagttcgag
     3181 ctggctggca gtccccatcg aggaccgtcc gggattttac ctacggggtt ttcgtgaccc
     3241 tcgtgacgcc cgacgcggct gggactggcg ccattgcgta acatgcttac cct
//
LOCUS       DASRJQ010000104         3160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_207083, whole genome shotgun sequence.
ACCESSION   DASRJQ010000104 DASRJQ010000000
VERSION     DASRJQ010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207083"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VFB89_02385"
     CDS             complement(<1..336)
                     /locus_tag="VFB89_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26180.1"
                     /translation="MKGPTFEHRNGMEIATFADAAEWETWLGAHGAERGELWLRIARR
                     DSGQTTVTADQALDVCLCYGWIDSLRRSDKGGYFLQRYSPRRPGSPWSKINAAKVEVL
                     LAAGRMRPAG"
     gene            complement(355..783)
                     /locus_tag="VFB89_02390"
     CDS             complement(355..783)
                     /locus_tag="VFB89_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26181.1"
                     /translation="MPVLHVSVTSPLSPQEALQRLTDFSTARAEAWAGVDEDNLIVHG
                     KGDGWAEITEGNKFAWERERYTWDASAGTVSAATLNSNIWAPGSGWNYTIMPDGAGSR
                     VEVTAVRKGYRIRGKLVGAVLSLIGKRLITSRTRAALAAR"
     gene            complement(890..1738)
                     /locus_tag="VFB89_02395"
     CDS             complement(890..1738)
                     /locus_tag="VFB89_02395"
                     /inference="COORDINATES: protein motif:HMM:NF018677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrase ferredoxin"
                     /protein_id="HZM26182.1"
                     /translation="MAGSAPPARRWLLLEHPGPWRTDAIAGAGINPDVLSTLVDRAGL
                     ATRILLVRRPGRIDRRAARRWILTALDSTTTTGRWRTDHDLLDAADAISAASPGQMET
                     SEPLILVCTHGVHDVCCALRGRPVALELASRWPDLVWECSHIGGDRFAPNVVLLPDGF
                     YYGNLDPATARDTVEAHLAGSVLTDRLRGMARYLPPAQAAAIAAYERFGPLGPSDVSV
                     RAIEHIGPHHGHGSETIVDLVVGPQLRLVRVRVLSVRRADAQLTCRASRETPATEYRI
                     VDFSSS"
     gene            complement(1795..>3160)
                     /locus_tag="VFB89_02400"
     CDS             complement(1795..>3160)
                     /locus_tag="VFB89_02400"
                     /inference="COORDINATES: protein motif:HMM:NF019619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZM26183.1"
                     /translation="EPMVAQTAEQTAEPMVALTAEPMVAQTAEPMVAQTAELTAGQTA
                     ERMVAQTAAPFAEDSALARLVAVERDEFAAEYWGQEPLLSPAADLPGGFSELLNANAI
                     DELLSKRGLRTPFLRVAKNGTTLADRAFTAAGGVGAGIADQVSDDRLVRLFADGSTLV
                     LQALHRVWPPILDFCQKLAAEMGHPVQANAYVTPPQNQGFSAHYDVHDVFVLQIEGEK
                     RWRIHRPVLASPLRDQPWNDFRAEVEDRAEETPLLETLLKPGDCLYLPRGHLHAATAL
                     GGVSTHLTLGIHVWTRFALAQQLVHQALQTLAEDPVMRASLPLGVEVSNPAETRQDFD
                     VVTALLTDAVEQADLDQISELLLDVARSNQRAAPIGPLKQLRDADGMTVDTQLVLRRH
                     LAAAVDHTGSRILVRSRAGDVEVTEDEVGRLEALLSEGVAAAGDVGLDLARRLLLAGL
                     AIAE"
BASE COUNT          578 a         1055 c          991 g          536 t
ORIGIN      
        1 ccccgccggc cgcatccgac cggccgcgag caaaacctcg accttggcag cgttgatctt
       61 tgaccacggg ctgccagggc gacgcggtga gtagcgctgg agaaaatagc cgcctttgtc
      121 actgcggcgc aggctgtcga tccagccgta gcacaggcat acgtccagcg cctggtccgc
      181 ggttacggtc gtttgtccgg agtctctgcg ggcgatccgc aaccacagct ccccgcgttc
      241 cgcgccatgc gcgcccagcc aggtttccca ctctgctgca tcagcaaagg tggcaatctc
      301 catgccgttt cggtgctcga acgtcgggcc tttcatggag cgaccttatg tctcctatcg
      361 agccgccagc gccgctctgg tgcgcgaagt gatcaagcgc ttgccgatca gcgataacac
      421 agcgccaacg agcttccccc tgattcggta ccccttgcgc actgccgtga cctcgaccct
      481 gctcccggcg ccgtcaggca tgatcgtgta gttccaccca ctgccaggtg cccagatatt
      541 ggagttcagg gtggccgccg agaccgtccc agccgacgca tcccaggtgt agcgttcccg
      601 ctcccaggcg aatttgttgc cttcggtgat ctcggcccaa ccgtcgccct tgccgtgcac
      661 gatcaggttg tcctcgtcga cgccagccca agcctcagct cgagcagtgc tgaaatcggt
      721 gagccgctgc agggcctcct gaggtgaaag gggtgaggtg acggaaacat gcaggaccgg
      781 caacgctctt cctcctcaga acttcatcag ggaagcggtc gtaaacaaga cgatatccag
      841 ccgattctgt gatgtttcga ccacttgcgg ggcaggcaaa tgtagttgat caacttgagg
      901 aaaagtcgac aatccgatat tccgtggctg gcgtctcgcg ggaggcgcgg caggttagct
      961 gagcatcagc gcggcgtacg gacagaaccc gcaccctcac caatcgcagc tgaggcccga
     1021 ctacgagatc gacgatggtc tccgatccgt gcccgtgatg gggcccaatg tgctcgatag
     1081 cccgaacaga aacatctgag ggcccgagcg gcccaaagcg ctcgtacgcc gcgattgcgg
     1141 cggcctgagc cggcggaaga tagcgggcca tgccgcggag ccgatcggtc agcacactac
     1201 ctgcgaggtg cgcttcgaca gtgtctcgcg cggtagccgg gtcgagattc ccgtagtaga
     1261 agccgtcggg cagcaagacc acattcggcg caaaccggtc gccgccgatg tgactgcatt
     1321 cccagaccag atcgggccag cgagacgcaa gctccagagc caccggacga ccacgaagcg
     1381 cgcaacagac atcgtgcacg ccgtgcgtac aaacaaggat cagaggttcg gatgtttcca
     1441 tctgccccgg ggaggcggcg cttatggcgt cggcagcatc caggagatcg tggtcggttc
     1501 gccaccggcc ggtagtcgtc gtcgagtcga gcgcagtcag gatccatcgc cgggccgcac
     1561 gccggtcgat ccgccccggt cggcgaacga gcaggattcg cgttgccaag ccggccctgt
     1621 cgaccaaggt cgaaaggaca tcggggttga tgcccgctcc cgcgatggcg tcggtccgcc
     1681 agggaccagg gtgctccagc agcagccagc ggcgcgccgg aggcgccgaa ccggccaacg
     1741 gatccgctcg taggcgcgct gcgtccgagc accggaacgc agggtctggt tgactcactc
     1801 ggcgatggcc aagccggcga gcagcagtcg gcgcgccaga tccagaccca cgtcaccagc
     1861 cgcagcgacg ccttcggaca gcagggcctc gagccgccct acctcgtcct cagtcacttc
     1921 tacgtcgccc gcgcggctgc gtaccaggat gcgggatccg gtgtgatcaa cagcagccgc
     1981 caggtggcga cgaagcacca gctgggtgtc gaccgtcatt ccatcggcat cgcgaagctg
     2041 tttgagtgga ccgatcggcg ccgcacgctg gttggatcgc gcaacgtcta acagcaactc
     2101 agatatctgg tcaagatctg cctgttcgac ggcgtcggtc agtagggcgg tgacaacgtc
     2161 gaagtcctgg cgggtctcag ccgggttgga tacctcgact ccaagtggca gggacgcccg
     2221 catcaccgga tcctcggcga gggtttgcaa ggcttggtgc acgagttgct gggccagtgc
     2281 aaagcgtgtc cagacatgga tgccgagagt caggtgggtg ctcaccccac cgagcgccgt
     2341 ggcagcgtgc aaatgtccgc gcggaaggta gaggcagtcg ccaggcttca gcaacgtctc
     2401 caggagcgga gtttcctccg cccgatcctc gacctcggct ctgaagtcgt tccaaggctg
     2461 atcacgaagc ggcgaggcca gcacaggccg atgaatccgc caccgcttct cgccctcaat
     2521 ctgcaagaca aaaacgtcat gtacgtcgta gtgagcgctg aagccttggt tctgcggcgg
     2581 cgtgacgtat gcgttggctt ggactggatg gcccatctcg gccgcgagct tttggcagaa
     2641 gtcgaggatg ggcggccaaa cccgatgcag ggcttgcagc acgagcgtcg agccgtcggc
     2701 aaacagtcgc accagcctgt catcgctcac ctggtcggct atgcccgcgc caacgccacc
     2761 cgctgctgtg aaggctctgt cggccagagt ggttccgttc ttggcgaccc ggaggaacgg
     2821 cgtgcgcaga ccgcgcttcg ataggagctc gtcgatcgca ttcgcattga gcagttcgga
     2881 aaacccgccc ggaagatctg cggcggggga cagcaacggt tcctgccccc agtactcagc
     2941 tgcgaactcg tcccgctcga cggcaaccag tcgagcgaga gcagagtcct cagcgaacgg
     3001 cgccgccgtc tgcgccacca tccgctccgc cgtctgcccc gccgtcagct ccgccgtctg
     3061 cgccaccatc ggctccgccg tctgcgccac catcggctcc gccgtcagcg ccaccatcgg
     3121 ctccgccgtc tgctccgccg tctgcgccac catcggctcc
//
LOCUS       DASRJQ010000105         2510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2463200, whole genome shotgun sequence.
ACCESSION   DASRJQ010000105 DASRJQ010000000
VERSION     DASRJQ010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2463200"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            288..737
                     /locus_tag="VFB89_02405"
     CDS             288..737
                     /locus_tag="VFB89_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26184.1"
                     /translation="MLKLTALAIAGISLLGVSGDKEWKATLAARDGSQIEGTADVKAK
                     SADSTAMDIEIQKATASTSYAWHMHNGNCAANGSVVGAPNTYPVLKTNDKGSGEVEAT
                     LAIPAPASGEYSVHVHAPSADTAAVGMTVACGDLKPAGGDLKPGGGQ"
     gene            complement(914..1444)
                     /locus_tag="VFB89_02410"
     CDS             complement(914..1444)
                     /locus_tag="VFB89_02410"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HZM26185.1"
                     /translation="MQIETMEDLLEDQLREIYGAERQFAAELPKLIWQTSSLSLRLAL
                     SDQFQETERQIDRLGEAFSALGKPPRGAYCPGIQSFLDEADRAARRSGRGVFLDAATV
                     ASAQRVARYAMATYSRAIAYAKLLGQPTIGELLTDSLTEERTTIDTLGRLSEDSAGDL
                     LVEPAETERHPILRQA"
     gene            complement(1495..1734)
                     /locus_tag="VFB89_02415"
     CDS             complement(1495..1734)
                     /locus_tag="VFB89_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26186.1"
                     /translation="MNELLNHEPGEVDDSLESPADATAPVVPDELIKGDWHRRLVLPD
                     QHGHLRLTPVKTSRGPRAGPATRIRYLDDPQRNSA"
     gene            complement(1752..1931)
                     /locus_tag="VFB89_02420"
     CDS             complement(1752..1931)
                     /locus_tag="VFB89_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26187.1"
                     /translation="MLIRLALASLVVALVAAVIGFSRAAADFGDINRSVAYVLVLVFC
                     VTLLLEAVTGRRPAS"
     gene            2248..>2510
                     /locus_tag="VFB89_02425"
     CDS             2248..>2510
                     /locus_tag="VFB89_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26188.1"
                     /translation="MTSGEIPIISGGVADLRSRSSGPWRQLWRISGWSLILLGGAGIV
                     GWLFTIRLLIQPVQSRGPLRPAAALGLLGLGLAALAYDRGFRRS"
BASE COUNT          452 a          762 c          830 g          466 t
ORIGIN      
        1 caagcggttt ctcgccgagg tgcatgccga agccgagccg gggcacgtgg tgcggggagc
       61 gtactgactg gccggacttc ggtccggcgc aatttcgcat gcttgcggca ccggtcttgg
      121 cttgataccc tgactctatc caatggcgct cgggcaacgg gccgggccgc cttggcggat
      181 ggaggcagtg gattcggtcc gtcgaaggcg cgtacggcgc tcccctggga gcgcggtcgc
      241 gcctttttga cgtcactgag cgttcaaacc tttggggagg atatctcatg ctgaaactca
      301 ctgcgctcgc gatcgcgggc atttcgctgc tcggcgtgtc gggggacaag gaatggaagg
      361 ccaccctggc cgcgcgcgat ggatcgcaga ttgaaggcac cgccgacgtg aaagccaagt
      421 cggccgactc caccgcaatg gatatcgaaa tccagaaggc gacggcgtcc accagctatg
      481 cgtggcacat gcacaatggg aattgcgccg cgaacgggtc ggttgtgggg gcgccgaata
      541 cttatccggt cctcaagacc aacgacaagg gttcgggaga agtcgaagcg accttggcga
      601 tacccgcgcc cgcttctggg gagtactcgg tacacgtcca tgcgccgagc gccgataccg
      661 ccgcggtggg gatgacggtc gcctgcggtg atctgaagcc tgctggcgga gatttgaagc
      721 caggtggcgg gcagtagtga gatcagtggc aaagaggcaa agaggctagg tagatctttg
      781 cctccttgcc tccttgccat cctaaaaaag ataggccccg ccgaccatgg accggggagg
      841 tcgcatggac ggcggtaggt cgacggcctc gcgagggtag ggagcgagtc gtcgggccta
      901 tcgttctata acttcatgct tgtcggagaa tcgggtgtcg ctcggtttcg gccggctcga
      961 ccagaaggtc gccggccgaa tcctccgata aacgcccgag agtgtcgatg gtggtccgct
     1021 cttctgtgag cgaatcggtc agaagctccc caatcgtggg ctggcccagc agtttggcat
     1081 aagcgatcgc gcgactgtag gtggccattg cgtaccgcgc gacgcgctgg gcactcgcca
     1141 cagtagccgc gtcgagaaag accccgcgtc ccgagcggcg ggccgcccgg tcggcttcgt
     1201 cgagaaagga ctggatgccg ggacagtagg ctccccgcgg cggctttccg agtgccgaaa
     1261 aagcttcgcc gagccggtcg atttgacgct cggtttcctg gaactggtcc gacaacgcga
     1321 gccggagcga gagggaagag gtctgccaga tgagcttggg cagctcggcg gcaaattgcc
     1381 gctccgcccc gtagatctcc cgcaattgat cctccagaag atcttccatc gtctcgatct
     1441 gcacgggccc ctctcctctc gtcgcggttc tcgactcagt gctcctgcta tcgatcaagc
     1501 gctattgcgc tgagggtcat ccaggtaccg aatccgggtt gcgggtccgg ctcggggccc
     1561 gcgggacgtt ttcaccggcg tcaaccggag gtgtccatgc tggtccggga gaacgagtcg
     1621 acggtgccag tctcccttga tgagctcgtc cgggaccacc ggtgcggtgg catcggccgg
     1681 gctctcgagc gagtcatcca cctcgccagg ctcgtgattc agcaattcgt tcatgaatcc
     1741 tctcagcctt gctatgacgc cggccggcga ccggtgaccg cttccagcag cagggtcacg
     1801 cagaagacca gcaccaggac ataggcgacg gaacgattga tgtcgccgaa gtcggcggcg
     1861 gcccggctga agccgatcac cgcagccacc agcgcgacca ccagagatgc gagtgccagt
     1921 cgaatcagca tagttccccc gatgcccggc gttgcaagga tccgaataag tcggcgaaag
     1981 tacgagagcg cggagagctt cccttgatgg gaaactcccc gcgccctcga cttccagagc
     2041 cgcttcgctc cggccgacag ggcaactcgc accgctctgc gagtgccatg tatatgaaaa
     2101 aaggcgccac ggcgtctcaa gcgacgtgtg gcgccttcgt ctgatggttc aagtgctcca
     2161 tccgacaagg cttggcgcca tgctaccggc gcctgcggac ttatgttaca aacttgagtt
     2221 ccaccgcgca agagcccaag ttcgaacatg accagcggtg aaatccccat cattagtggc
     2281 ggtgtggccg atcttcggtc acggagttcg gggccatggc gccagctttg gcggatcagc
     2341 ggctggtcgc tgatcctttt gggcggggcc gggattgtcg ggtggctgtt cacgattcgg
     2401 ctactgatcc agccggtgca atcgcgtggg ccgcttaggc cggctgcggc gctcggactg
     2461 ctggggctcg gcctcgcggc actcgcgtac gaccgaggct tccgccgctc
//
LOCUS       DASRJQ010000106         3075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1618804, whole genome shotgun sequence.
ACCESSION   DASRJQ010000106 DASRJQ010000000
VERSION     DASRJQ010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1618804"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /gene="tilS"
                     /locus_tag="VFB89_02430"
     CDS             <1..292
                     /gene="tilS"
                     /locus_tag="VFB89_02430"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF023162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HZM26189.1"
                     /translation="TAPSRQERAATTAWFELGRLRVRALRSGERMRPLGGQGKRLIVR
                     CLQEARIPRSRRATWPALEHEGTVVWVPGVCRSDRLLPAPGTEALRVDAQFP"
     gene            270..833
                     /gene="hpt"
                     /locus_tag="VFB89_02435"
     CDS             270..833
                     /gene="hpt"
                     /locus_tag="VFB89_02435"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HZM26190.1"
                     /translation="MSTLSSPEVLRRTGGRSIKSIVFDEREIARRVRELGAEVTAAYP
                     DGDLLVLGLLKGSFIFLSDLVRTIERPMQIDFLVATSYGADKVSSGTVRLLYDPETKL
                     EGKHILLVEDIIDSGRTLQRLLGLLGGRRPRSLAICALLDKQLALVRPPELRFVGFRA
                     PPAFLVGYGLDHAENYRHLPFIADLDS"
     gene            834..2822
                     /gene="ftsH"
                     /locus_tag="VFB89_02440"
     CDS             834..2822
                     /gene="ftsH"
                     /locus_tag="VFB89_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HZM26191.1"
                     /translation="MADRLPTRPPRPSWGNLSKNLALWLLVALLALALFQMMSRQRNP
                     TQEFSYTEFSRQLDQGNIARVEIYDGKRLEGDFKTPVAQDGRSAKSFTVLLPIANSEV
                     FLKRLDESGVQIVAREPKGGITAIIIAALPWIVILGLWFFLLRQLQAGGSRAFAFGKS
                     KAKLLAGDTPKITFADVAGADEAKVELQEIIEFLKDPQKFTRLGGRLPKGALLVGPPG
                     TGKTLLAKAVAGEAGRPFFSMSGSDFVEMFVGVGASRVRDLFEQGKAHAPCIIFIDEI
                     DAVGRHRGAGLGGGHDEREQTLNQLLVEMDGFESNDGVILLAATNRPDVLDPALLRPG
                     RFDRQIVVDAPDVRGREQILRIHTRKIPLGTDVRLDIIAKGTPGMAGADLANLVNEAA
                     VLAARKNKSLVDHRDFEEAKDKVMLGVERRSLVLTEDERKLTAYHEAGHAIVALKTPG
                     SDPIHKVTIVPRGRALGLTASLPEVDRHNYTKDWLIGSLAMFFGGRVAEEIIFGPEKV
                     TTGAGNDIERATALARRMVTQFGMSERIGPLAVGDKEQEIFLGREFAQRREISERTAE
                     AVDDEVKRLVDEAYARAMEIVQANRELLDRIAVALLERETIDREDLDRLMKGEPLPPR
                     VLAPEPFNPQTPTTPTKPATQPARAPVLGAPPAEPAGA"
     gene            2819..>3075
                     /locus_tag="VFB89_02445"
     CDS             2819..>3075
                     /locus_tag="VFB89_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26192.1"
                     /translation="MNVVPLTYGTSRSLRDTLVEHGWSPEAAAAAAGGADPLAFHLTD
                     LDQDALEALVHFSGKLGLEVLTGERWAIMAGSRSRLSAFARP"
BASE COUNT          531 a          970 c         1032 g          542 t
ORIGIN      
        1 taccgcaccg tcccggcagg agagggccgc gacgacggca tggttcgagc ttggtcggct
       61 cagggtgcga gccttgagaa gcggtgagcg gatgaggccg ctcggaggtc agggaaaacg
      121 tctgattgtg cgctgcctgc aggaggctcg cattccccgg agtcggcggg caacctggcc
      181 ggcactggag cacgagggca cggttgtctg ggtccccggt gtttgccgtt ccgatcgtct
      241 ccttcctgcc ccgggcacgg aggcactgcg tgtcgacgct cagttcccct gaggtcctcc
      301 gccgcaccgg cggacgcagc ataaagtcga tcgttttcga cgagcgggag atcgcccgtc
      361 gagtgaggga gctgggcgcc gaggtcacgg ctgcctatcc cgatggcgac ctgctcgtcc
      421 tcgggctgct caagggaagc ttcattttcc tgagcgatct ggtgcggacg atcgagcggc
      481 cgatgcagat cgacttcttg gtcgctactt cctatggtgc cgataaggtc tcgagcggca
      541 cggtgcggct tctttatgac ccggaaacca agctggaggg caaacatatc ctgctggtag
      601 aggatataat tgatagcgga cggacgctgc agcgccttct cgggctgttg ggcgggcggc
      661 ggcccaggag cctggcaatc tgcgcgctcc tcgacaagca gctcgcgctc gtgcgccctc
      721 cggagcttcg cttcgtgggc ttccgagcgc cgccggcgtt tctagtcggt tacggactcg
      781 accacgcgga gaattatcgt caccttccgt tcattgcaga cctggattcg tagatggctg
      841 acagacttcc aacccgaccc cctcgtccca gctgggggaa cttgagcaag aacctcgcgc
      901 tgtggctgct cgtggccttg ctcgcgctcg cgctcttcca gatgatgagc cgccagcgca
      961 atcccacgca ggagttctcc tacaccgagt tcagtcgtca gctcgatcag ggcaacatcg
     1021 cccgggtcga gatctacgac ggcaagcgcc tcgaaggcga cttcaagact ccggtggcac
     1081 aggacgggcg ctccgcgaag agcttcacgg ttctgctccc catcgcgaac agcgaagtgt
     1141 tcctgaaacg gctcgacgag tccggcgtgc agatcgtagc ccgcgaaccg aagggtggaa
     1201 tcactgccat catcattgca gccttgccgt ggatcgtgat cctgggactc tggttcttcc
     1261 tgctgcgcca gcttcaggcc ggcgggagcc gcgcgttcgc gtttggaaaa tcaaaggcca
     1321 agctgctcgc aggggacacg cccaagatca cctttgccga cgtggcgggc gcggacgaag
     1381 ccaaggtcga gctgcaggag atcatcgagt tcctcaagga tccgcagaaa ttcacccgtc
     1441 tgggtggccg gctccccaag ggcgcgctgc tggtcgggcc tccgggcaca ggcaagacgc
     1501 ttctggccaa ggccgtcgct ggtgaggccg gaaggccgtt cttctcgatg tccggctccg
     1561 acttcgtcga gatgttcgtg ggcgtgggcg cgagccgggt acgtgatctg ttcgagcagg
     1621 gtaaggctca cgcgccctgc atcatcttca tcgacgagat cgatgcggtg ggccgtcatc
     1681 gcggcgccgg tctcggcggt ggtcatgacg agcgggagca gaccctgaat cagctgctgg
     1741 tcgagatgga cgggttcgaa tcgaacgacg gcgtcatcct gctcgcggcc accaaccggc
     1801 cggacgtgct cgatcccgct ctgctacggc ccggccggtt cgaccgccag atcgtggtag
     1861 atgcaccgga cgtgcggggc cgggagcaga tactccggat ccacactcgg aagatcccgc
     1921 ttggcaccga cgtgcgcctc gacattatcg caaagggaac accgggaatg gccggcgccg
     1981 atctggccaa tttggtcaac gaggcggctg tgctcgcggc ccggaagaac aagagcctgg
     2041 tggaccatcg cgacttcgaa gaggccaagg acaaggtgat gctcggcgtc gagcgccgga
     2101 gcctggtcct cacggaagac gaacggaagc tcactgcgta ccacgaggcg gggcacgcca
     2161 tcgtggctct caagacgccc ggcagcgatc ccattcacaa ggtgaccatc gtcccgcgtg
     2221 gccgtgcgct cggtcttacc gcgtcgttgc ccgaggtcga tcgccacaac tataccaagg
     2281 actggctgat cggcagcctc gcgatgttct ttggcgggcg cgtcgccgag gagattattt
     2341 tcggcccgga gaaggtgacc acgggggcgg gcaacgacat cgagcgtgcc acggccctgg
     2401 cgcgtcgtat ggtgacacag ttcgggatga gcgagcgtat cggtccgctc gccgtaggcg
     2461 acaaggagca ggagatcttc ctcggtcgcg aattcgcgca gcggcgcgag atctccgagc
     2521 ggactgcgga agcggtggac gacgaggtca agcgactggt ggacgaggcc tacgccaggg
     2581 cgatggaaat tgtccaggcg aaccgtgagc ttctcgatcg cattgcagtg gcgttgctcg
     2641 agcgcgagac catcgaccgc gaagacctcg accggctgat gaagggcgag ccgcttccac
     2701 ccagggtgct ggcgccggag ccgttcaatc cccagacgcc gacgacaccc accaagcccg
     2761 cgacccagcc ggcccgcgcg ccggttctcg gtgcgccacc ggctgagccg gccggcgcgt
     2821 gaatgtcgtc ccccttacgt atggcacttc ccggtcgctc cgagacaccc tggtagaaca
     2881 cggctggtcg ccggaggccg ccgcggccgc cgcgggcggc gcagacccgc tggcctttca
     2941 tctaactgat ctggaccagg acgccctcga ggccctggtc catttttctg gtaagctcgg
     3001 gctcgaggtc ttaaccggag agcggtgggc catcatggcc gggagccgtt cgcgcctgag
     3061 cgcgttcgcg cggcc
//
LOCUS       DASRJQ010000107         4171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3588273, whole genome shotgun sequence.
ACCESSION   DASRJQ010000107 DASRJQ010000000
VERSION     DASRJQ010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3588273"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..1463
                     /gene="nuoD"
                     /locus_tag="VFB89_02450"
     CDS             153..1463
                     /gene="nuoD"
                     /locus_tag="VFB89_02450"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HZM26193.1"
                     /translation="MSRRTVEYTLSTPGLNAQGEVEQIPLGILKDTQEYKIPGEFDEG
                     FGSEHMLINIGPQHPATHGVLRLVIELDGETVIRVIPHIGYLHSGFEKLGEYRQYNQI
                     IPLTDRTDYLSPMANNVGFALAVEKLMGLEITERCEVLRVIACELSRIISHLVWLGTT
                     GIDLGAYTPFLWSFQERERVYNLQEAWTGARLTTSVTRVGGMMADVPTGWTDALREFC
                     RTFPSTLKEVDTMFTRNAVWCGRTQDVGAMSREDAINYSLSGPMLRASGVDYDVRKDR
                     PYLGYETYDFDVPIGEHGDIYDRYRVRLEEMWQANRILEQALERLEKLKGAPINVSDP
                     RVILPPKSRAMSDMEAMIFHFKQVMEGVKPPVGEAYMGVENPKGELGTYLVSDGTAKP
                     VRWRIRPPSFINLAALPKMCEGALLSDVIAINASVDIVMGEIDR"
     gene            1460..2005
                     /gene="nuoE"
                     /locus_tag="VFB89_02455"
     CDS             1460..2005
                     /gene="nuoE"
                     /locus_tag="VFB89_02455"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HZM26194.1"
                     /translation="MSEPLTRLEPRSPRGHSGGHAMPHEPVFVGALREELEKLFPKYP
                     TKQACLLPALWIVQRERGWISEAAMAEVAQVLGLTPAVVKGVVTFYTMYHTHPVGRHF
                     IQVCTTSPCGLCGAEDTLRALLEATGCGELGATSPDGRFTVIEVECLGACGFPTPILV
                     DDDFIESVTPDRVGAILEGYL"
     gene            2005..3312
                     /gene="nuoF"
                     /locus_tag="VFB89_02460"
     CDS             2005..3312
                     /gene="nuoF"
                     /locus_tag="VFB89_02460"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HZM26195.1"
                     /translation="MGFPHPSHPRETVVLSEHFGEAAARSYQGWIERGGYAGLRQALA
                     MAPDEVTNVVKESGLRGRGGAGFPTGLKWSFMPKADGKPHYLVCNADESEPGTFKDRE
                     IMRWTPHALIEGCAIASYAIGAETCYIYIRGEFTEPLRIMETAVAEAYASGALGGNVL
                     GSGRRIEMVLHRGAGAYICGEETGLMSSLEGKRGNPRSKPPFPAQAGVFGMPTTVNNV
                     ETLAAVPHIIVRGAGWYKGLVMGSPKSPGTKLISVCGHVQKPGNYEITLGFPMKDLVF
                     ELAGGMRPGRSLKAVIPGGSSVPIMSAAEVEDCPTDYENIQANGSMLGSGGMIVMDDS
                     TDLVYQIWRLAKFYAHESCAQCTQCREGTAWTTKVLERILAGKGKTEDLDLLLDLSEN
                     MTGKTICVLSDSCAAPVVSGIQKFRSEFEAYISKAQKPALVAV"
     gene            3341..>4171
                     /locus_tag="VFB89_02465"
     CDS             3341..>4171
                     /locus_tag="VFB89_02465"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZM26196.1"
                     /translation="MADDLVTVTIEGIQIAVPKGTMIIEAAKQAGVLVPHYCYHPSLP
                     APAVCRMCLVEVEKQPKLAPACVTAVADGQVVHIGSEKAKAARQSVLELLLINHPLDC
                     PICDQAGECELQDYVFQEGRSGTRYAEYAKRYSPVEDFGPDILYVPNRCILCTRCVRF
                     MESVAQDPVLNVSERGDRAYIGIAEDQPLDHPWSGNVVDLCPVGSLLSKDFLHKARAW
                     DLDKTASVCPGCTQGCNITIDTRDDVVVRLRPRPNLEVNRHFICDHGRMNYRWMNRGD
                     R"
BASE COUNT          785 a         1321 c         1357 g          708 t
ORIGIN      
        1 cacttcagcc gagccgaaca aacccggcag gcgctcgcgg cgagtccgga aacacactat
       61 tcacttaccg agctgtccat tgcctcggcc taccacgaac tgcccaagga tatgcgagag
      121 cggctgcgcc gcggcgaacg ggggttcctg aaatgagtcg ccgcaccgtc gagtacactt
      181 tgtcgacgcc cggactcaac gctcagggcg aggtggagca gatccccctc ggcatcctca
      241 aggacaccca ggagtacaag atccccgggg agttcgatga gggcttcggc tccgaacaca
      301 tgctgatcaa catcggtccg cagcacccgg ccactcacgg ggtgctacgg ctggtcatcg
      361 agctggacgg cgagactgtg atccgcgtaa tcccgcacat cggttatctc cactcgggtt
      421 tcgagaagct gggagagtat cgccagtaca atcagatcat tccgctgacc gatcggaccg
      481 actacctgtc gcccatggcg aacaacgtcg ggttcgcgct ggcagtcgag aagctgatgg
      541 ggctcgagat caccgagcgc tgtgaggtcc ttcgggtcat cgcgtgcgag ctgagccgca
      601 ttatctcgca tctggtctgg ctcggcacca ccggaatcga tctcggcgcg tacacgccgt
      661 tcctctggtc gttccaggag cgggaacggg tctacaacct ccaggaggca tggaccggtg
      721 cgcggctcac caccagcgtg acgcgggttg gcgggatgat ggctgatgtg ccgaccggat
      781 ggacggatgc cctgcgggag ttctgccgga cgttcccgag caccctgaaa gaggtggaca
      841 cgatgttcac ccgcaatgcc gtgtggtgcg ggcgaaccca ggacgtcggg gcgatgtcac
      901 gcgaggacgc gatcaactac tcgctgtccg ggccgatgct ccgcgccagc ggcgttgact
      961 acgatgtgcg gaaggatcga ccctacctcg gctacgagac ttacgacttc gacgtgccca
     1021 tcggcgagca cggcgatatc tacgaccgct accgggtccg gctggaagag atgtggcagg
     1081 ccaaccgcat cctcgagcag gctttggagc ggctggagaa gctcaaaggt gctccgatca
     1141 acgtctccga tccgcgcgtc attcttccgc ccaagtcccg cgcgatgagc gacatggagg
     1201 ccatgatctt ccacttcaag caggtcatgg aaggcgtgaa gcccccggtg ggagaagctt
     1261 acatgggcgt ggaaaatccc aagggcgagc tgggcaccta tctcgtgtcg gacggaaccg
     1321 ccaagccggt gcgctggcgc attcgtcctc cttcgttcat caacctcgcg gcgcttccca
     1381 agatgtgcga aggcgcgctc ctgtccgatg tcatcgccat caacgccagc gtggacatcg
     1441 tcatgggcga gatcgaccga tgagcgaacc cctcacccgg ctcgagcccc ggtcgcctcg
     1501 cgggcacagc ggcgggcacg cgatgccgca cgagcctgtc ttcgtcggtg cgctccggga
     1561 agagctggag aagctcttcc cgaaataccc gaccaagcag gcgtgcctgc tcccggcgct
     1621 ctggatcgtg cagcgggagc ggggctggat ttccgaagct gcgatggcgg aagtggcgca
     1681 ggtgttggga ctgaccccgg cggtagtgaa gggggtggtc accttctaca cgatgtatca
     1741 cacgcacccg gtaggccggc atttcattca ggtctgcacc acgtcaccct gcgggctgtg
     1801 cggcgcggag gacacgctcc gcgcactgct cgaggcaacc ggttgcggcg agctcggcgc
     1861 caccagcccg gacggccgtt ttaccgtcat cgaggtcgaa tgcctagggg cctgcggctt
     1921 cccgacgccc attctggtag atgacgattt catcgaaagc gtaacgccgg acagagtggg
     1981 cgcgattctc gagggatatc tctgatgggc ttcccccacc cgagtcaccc gagggagacc
     2041 gtggtgctct cggagcactt cggcgaggcc gcggcccgga gctaccaggg ctggatcgag
     2101 cgcggcggtt acgccgggct ccgtcaggcg ctggcgatgg ccccggacga ggtgacaaac
     2161 gtcgtgaagg agtcgggcct aagaggccgc ggaggcgccg gtttccctac cggcctcaag
     2221 tggtcgttca tgcccaaggc cgacggcaag ccgcactatc tggtctgcaa cgccgacgag
     2281 tccgagccgg gcacgttcaa ggaccgcgag atcatgcggt ggacgccgca cgccctgatc
     2341 gagggctgcg ccatcgcgtc gtacgctatt ggcgcggaaa cgtgctacat ctatatccgc
     2401 ggcgaattca ccgagcccct ccggatcatg gaaacggcgg tcgccgaggc gtacgcgagc
     2461 ggtgcattgg gcggaaacgt gctgggaagc ggaaggcgga tcgagatggt cctccaccgc
     2521 ggcgctgggg cgtacatctg cggcgaagag accggactca tgagctcgct cgaaggcaag
     2581 cgaggcaatc cgcgcagcaa gccgccgttc cccgcgcaag ccggcgtgtt cggcatgccg
     2641 accacggtca acaatgtcga gaccctggcc gccgtgccgc acatcatcgt gcgcggcgcc
     2701 gggtggtaca aaggtctggt gatgggcagc cccaagagcc ccggcaccaa gctgatctcc
     2761 gtttgcggcc acgtgcagaa gcccggcaac tacgagatca ccctcggctt cccaatgaaa
     2821 gacctggtct tcgagctggc cggcggcatg cggccgggac gcagcctcaa ggcggtgatc
     2881 ccgggaggct cgtcggttcc tatcatgagc gccgccgagg tggaggactg tcccaccgat
     2941 tacgagaaca tccaggccaa tggctccatg ctcgggtcgg gcgggatgat cgtaatggac
     3001 gactcgaccg acctggtgta tcagatctgg cggctggcca aattctacgc tcacgaatcc
     3061 tgcgctcaat gcacccagtg ccgtgaggga acggcgtgga ccacgaaggt cctcgagcgg
     3121 attctggcag ggaaggggaa gaccgaggat ctcgatcttc tcctcgatct ctcggagaac
     3181 atgacgggca agacaatttg cgttctgagc gactcctgcg ccgcgccggt ggtgagcggc
     3241 attcagaagt tccgctcgga gttcgaagcc tacatctcga aggcgcagaa gccggcgctt
     3301 gtagccgtct gagcaccaac cgcatccatc ggtgagctga atggccgacg acctggtaac
     3361 cgtcaccatc gaaggcatac agatcgcggt gccgaagggc accatgatca tcgaggctgc
     3421 caagcaggcc ggcgtgctgg tgccgcacta ctgctaccat ccatccctgc cggcgcccgc
     3481 ggtctgccga atgtgtctgg tggaggtcga gaagcagccc aagctcgccc ctgcctgcgt
     3541 aaccgcggtc gccgatggtc aggtcgttca catcgggagt gagaaggcca aggcggcgcg
     3601 gcagagcgtg ctcgagcttc tcctcatcaa ccacccgctc gattgcccga tctgcgatca
     3661 ggccggcgag tgtgagctac aggattacgt gtttcaggag ggccggtcgg gcacccggta
     3721 cgccgagtac gccaagcgat acagcccggt agaagacttc gggccggaca tcctatacgt
     3781 ccccaatcgc tgcatcctct gcacccgctg cgttcgcttc atggagagcg tggctcagga
     3841 tcccgtcctg aatgtctcgg agcgtggcga ccgcgcctac atcggcattg cggaggatca
     3901 gccgctcgat catccgtggt cgggcaacgt ggtcgatctg tgtccggtgg ggtcgctcct
     3961 gtccaaggat ttcctgcaca aagcacgcgc ctgggatctc gacaagaccg cgagcgtctg
     4021 ccccggctgc acgcagggct gcaacatcac gatcgacacc cgggacgacg tggtggtccg
     4081 gctccggccg cggcccaacc tcgaggtcaa ccgccatttc atctgcgacc atggccggat
     4141 gaactaccgc tggatgaacc ggggcgaccg g
//
LOCUS       DASRJQ010000108         2309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1893659, whole genome shotgun sequence.
ACCESSION   DASRJQ010000108 DASRJQ010000000
VERSION     DASRJQ010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1893659"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VFB89_02470"
     CDS             complement(<1..233)
                     /locus_tag="VFB89_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26197.1"
                     /translation="MPAGYRSRGSGAPFQGTPNNEAERALRLAVIARRLSFGTRTEEG
                     SAAYAATLSVVETCRRRRVDPWSYIADTLARART"
     gene            668..2020
                     /gene="ltrA"
                     /locus_tag="VFB89_02475"
     CDS             668..2020
                     /gene="ltrA"
                     /locus_tag="VFB89_02475"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HZM26198.1"
                     /translation="MARPRRLAQGLPTPRERVRKLQTSLQAKAKAEPAFRFYSLWDKI
                     CRPDVLTEAYRRCRHNAGAPGADGQTFEAIEEHGVEEWLEELREELRNGRYRPKPLLR
                     VWIPKSSGGQRPLGIPAISDRVAQMAALLVIGPIFETDLLVNQYGFRPGMDAKMAIRR
                     AFWHITDHGRTEVADVDLKDYFNTIPHGPLMRCVARRIADGTVLAVIKAWLTAPIIER
                     GKRGMQQSTEARDSNRGTPQGGIISPLLANLYFRRFLLAWNSHGHRDRLGAYVVNYAD
                     DFVICCRPGNGEAALATMRHLMTRLGLTVNEAKTRLIRIPDGSCDFLGYTIGRFHGRD
                     GGAYIGTRPSRKAVRALLRKIHDATTPQKHADTPEHRVAEISSLLRGWAGYFNQGPVT
                     RIYETARRYTERRVRRWLMRRRGQKGTGYRQYPDEYLYQKLGLYALPRRRADPPSAKA
                     "
     gene            complement(2025..>2309)
                     /locus_tag="VFB89_02480"
     CDS             complement(2025..>2309)
                     /locus_tag="VFB89_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor IIS helical bundle-like
                     domain-containing protein"
                     /protein_id="HZM26199.1"
                     /translation="LKRACKLHRDHETEKIRALAREILNDWSAIVAFVTNHALPPSRV
                     DDWRGSDRSLGSCRSPAAFARAGASFLTMAPFPVAAHQTGRADFLHPAFT"
BASE COUNT          455 a          692 c          805 g          357 t
ORIGIN      
        1 gttctggcgc gggcgagagt gtcggcgatg tatgaccagg gatcgacgcg gcgccttcgg
       61 caggtttcga cgacggacag ggtggcggcg taggcggcgc tcccttcctc ggttcgcgtt
      121 ccgaagctga ggcgccgggc gatgacggcc aggcgcaggg cgcgctcggc ctcgttgttg
      181 ggtgtgcctt ggaaaggtgc accagatccc cgggagcgat acccggccgg caaactctcg
      241 ctccagccgg aagcacttgg agcggaccag gaggcgacaa atggtctgaa gcactctgag
      301 aaaaggccct gaagagggtc agcggacctc gcgggccgca acgtgagtga acatcgagca
      361 ggccccgaaa cggccaatgc ggaagccgac ccgccccaat aacggggaag gccgctgctc
      421 ccggggaagc gagcgagaaa cgcacccggg ggttccgccg gggtaatgat ggcagcacgc
      481 gagggcaagg tctggtgtgc aacaagggaa gtctgcctcg gcggccacgg gcggcacccg
      541 gatggccaac gacgaccccg cgagggaggg cgtcggtcgg ggcagatggc ggatgggttc
      601 gcagtaccgc tgaggccggg taatgccggt ggagggaagg agccctgacc tgggacagga
      661 gaggaaagtg gcaagacccc ggagattggc ccaaggccta ccaactccgc gtgaacgggt
      721 tcggaagctg cagacttctc tgcaggcgaa agctaaggct gaaccggcct tccgcttcta
      781 cagcctgtgg gacaagatct gccggccgga cgtcctgacc gaggcgtatc ggcgctgtcg
      841 gcacaatgcc ggcgccccgg gcgcggacgg ccagacgttc gaggcaatcg aggagcacgg
      901 cgttgaagag tggctggaag agttgcggga ggagctgagg aacggtcgct accggccgaa
      961 gcccctcctg cgcgtctgga taccgaaaag cagcggcgga cagcggccgc tcggcattcc
     1021 cgccatttcc gatcgtgtgg cccagatggc tgcgctgttg gtgatcggcc ccatcttcga
     1081 gacggacctg ctggtgaacc agtacggttt ccgacccggg atggatgcca agatggccat
     1141 ccgtcgggct ttttggcaca tcacggacca cggacggact gaagtagcgg acgttgatct
     1201 gaaggactac ttcaatacga tcccacacgg accgctgatg cgctgcgtgg cacgccgtat
     1261 cgccgacggc acagtgctgg cggtgatcaa ggcctggctc acagctccaa tcatcgagcg
     1321 cggcaagcgc ggaatgcagc agagcacgga agccagggac agcaaccgtg ggacgccgca
     1381 gggcggaatt atttcgccat tattggcgaa tctgtacttc cgccgctttc tgttggcgtg
     1441 gaacagccac ggtcatcgcg atcgactcgg ggcatacgtc gtcaactatg ccgatgactt
     1501 cgtcatctgc tgtcgacccg gcaacgggga agcggctctg gcgacgatgc ggcacctgat
     1561 gacacggctc gggctcacgg tgaatgaggc caagacccgg ctgatccgga taccggacgg
     1621 aagctgcgac ttcctcggct acacgatcgg ccgtttccac ggacgggacg gcggggccta
     1681 catcggcacc cgtccgtccc gcaaagccgt ccgggcattg ctgcgcaaga tccatgacgc
     1741 aacgacgccg caaaagcacg ccgacacgcc ggaacaccgg gtcgccgaga tcagcagctt
     1801 gctgcgcggc tgggcagggt acttcaacca aggccctgtc acccggatct acgagacggc
     1861 gcgccggtat accgagcggc gcgtccgacg ctggttgatg cgacgtcgcg gtcagaaagg
     1921 caccgggtac cgccagtacc cggacgagta tctttaccag aaactcggcc tttacgccct
     1981 gccgcgccga cgtgccgacc cgccgagcgc gaaggcttga cgtctcaggt gaaagccgga
     2041 tgcaggaaat ctgcacgtcc ggtttgatga gcggcgactg gaaacggagc catggtcagg
     2101 aatgaggcac cggcacgggc gaaagccgcc ggagaacggc aactccccaa tgacctgtcg
     2161 ctaccgcgcc agtcgtcgac tctacttggc ggcagggcat ggttggtgac gaaggcgacg
     2221 atggcgctcc agtcgttgag gatttcacgg gcgagggcgc ggattttctc ggtctcgtga
     2281 tcgcggtgga gcttgcaggc ccgtttcag
//
LOCUS       DASRJQ010000109         3523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1054946, whole genome shotgun sequence.
ACCESSION   DASRJQ010000109 DASRJQ010000000
VERSION     DASRJQ010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054946"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /gene="purH"
                     /locus_tag="VFB89_02485"
     CDS             <1..272
                     /gene="purH"
                     /locus_tag="VFB89_02485"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HZM26200.1"
                     /translation="AGVGAGQMSRIDSVRIAVDKSAAAGIDLGRAVMASDAFFPFADG
                     ARLAIEAGVQAIIQPGGSQRDGEVIDACDEAGVAMVFTARRHFRH"
     gene            324..647
                     /locus_tag="VFB89_02490"
     CDS             324..647
                     /locus_tag="VFB89_02490"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZM26201.1"
                     /translation="MREPHHPARAEIELSAVLHALSDPARLEIVRRLADGDEPSCGMF
                     ELGLSKATLSHHFKVLRESGVIHTRPEGRKRLLSLRTEDLETRFPGLLDAVLAAKRRR
                     AKSAA"
     gene            1129..1200
                     /locus_tag="VFB89_02495"
     tRNA            1129..1200
                     /locus_tag="VFB89_02495"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1162..1164,aa:Glu,seq:ttc)
     gene            complement(1198..1575)
                     /locus_tag="VFB89_02500"
     CDS             complement(1198..1575)
                     /locus_tag="VFB89_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26202.1"
                     /translation="MNARLFWKSLLVQAVAVAIPFAILGIALDRDFFEDWGWAVGPAV
                     WLLCSVVTARVLALPLGYVLFSAVAGGVAGLLVMLATSHLPGMGAALLVFAASCGSYD
                     PDAEAEAQREWEAERAAKRTAAK"
     gene            1603..1675
                     /locus_tag="VFB89_02505"
     tRNA            1603..1675
                     /locus_tag="VFB89_02505"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1636..1638,aa:Val,seq:tac)
     gene            1841..2353
                     /locus_tag="VFB89_02510"
     CDS             1841..2353
                     /locus_tag="VFB89_02510"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZM26203.1"
                     /translation="MDAPANEAPYRLIHDVSFGQGVVVAAFVNLYGCEIGAGTRIGPF
                     VEIQKGARVGTRCKVQSHTFICEGVTIGDEVFVGHGVLFVNDKRPRATNAAGELQGDA
                     DWELLDTCVESGATIGSGAVILGGIRLGTGAMIGAGALVAKDVPPHATVAGNPARLLH
                     GVPRHDLGSA"
     gene            complement(2315..3031)
                     /locus_tag="VFB89_02515"
     CDS             complement(2315..3031)
                     /locus_tag="VFB89_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HZM26204.1"
                     /translation="MYFKQPLTDRRVSVVGYGSVRSPAQIDDPDFARQEAIVASFCQR
                     RGWDLVALLRDVEPRRSRAWGRPSLISALERIGRGEADCLVTTELSRLCPSIAELGSI
                     LVAIEERDVRLVSLDPALDTRNPVGRAVLRALVSMSDWERERRAEMTAAARSKIPVTG
                     TISSTLRRRIVRLRGAGLTLQAIADMLNEEGVPTVRGGATWRPSSVQAAAGYKRPEPW
                     DIGERTRGSSRPKVMAGDPV"
     gene            complement(3102..>3523)
                     /locus_tag="VFB89_02520"
     CDS             complement(3102..>3523)
                     /locus_tag="VFB89_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26205.1"
                     /translation="ILPMAPQPPATAPRSDAAAPSPSAPRAERASALPQFALGLLAGD
                     RVAFTGLAASVHSTRPAGDGIPVAAPSPDVRQSDSTPPGASAGAGVGGSTGSSAASPM
                     DLRSALALLLVGAWSLLVLALDSYRFNPFLALPERPG"
BASE COUNT          577 a         1200 c         1213 g          533 t
ORIGIN      
        1 cggcgggggt cggcgccggg cagatgagcc ggatcgactc ggtccggatc gcggtcgaca
       61 agtccgcggc cgcgggcatc gacctcggca gggcggtgat ggcctccgac gcgttcttcc
      121 cgttcgccga cggagcccgg ctggcgatcg aggccggcgt gcaagcgatc attcagccgg
      181 gcggctcgca gcgcgacggc gaggtgatcg acgcctgtga cgaagcgggc gttgcgatgg
      241 tcttcacggc ccgtcgccac ttccgccatt agctcttaga cattcatcga actgtcgcta
      301 gactcgaccg cagtagcaag cgcatgaggg agccccacca cccagcccgc gcggagatcg
      361 agctctccgc cgtcctacac gcattgagcg acccggcccg tctggagatc gtgcggaggc
      421 tcgccgacgg cgacgagcca tcctgcggga tgttcgagct cggcctgtcg aaggcaacgc
      481 tctcgcacca cttcaaggtc ctgcgcgagt ccggagtgat ccacacccgc cccgaaggcc
      541 gcaagcggct gctctcactc cgcaccgaag acctggaaac gcgcttcccc gggctgctgg
      601 acgccgtgct ggcggccaag cggcgccggg cgaagtcagc tgcctgaaac cgcgcgcgag
      661 cgtcgcgcgg cgcaacgcac ggtggaggca cccgcggtcg actgatgcgg ccaagcggcg
      721 ccgggcgagc ctgtcgcccg gctccgcgta gatgggtcgt ctgggactcg aacccagaac
      781 cttcggatta acctgccgca ccggctttca ccggccaccc gcctcggcgg gcttgcggtc
      841 tggaccatct cttcgccctc gactcgacgt tagggcgggc ggcgtatggc ctctgagggc
      901 tctccgactc tgtcgggttg cctgctgatc gcccaatcct gaggattgtc acgctaggcg
      961 tacctcaggc tctaaggggg ttccagcaaa cagccgccga ccaaccagcg gtttcccgtc
     1021 tggcgctcct ttcaaagtcc gctgctctac cagttgagct aacgacccag ccccgacatt
     1081 atggcgagcg cgaattgcca tggctcgcgc ctagaatcgg tcgagcgcgg ccccgtggtg
     1141 taacggttag cacgcgaggt tttcaacctc gtagcgcggg ttcgattccc gccggggcta
     1201 cttcgcggcg gttcgcttcg ccgcccgctc cgcctcccac tcccgctgcg cctcggcctc
     1261 cgcgtccggg tcgtagctgc cgcacgaggc ggcgaagacc agcagggccg cccccatgcc
     1321 gggcaggtgg gaggtggcca gcatcaccag cagccccgcc actccgccgg cgaccgcgct
     1381 gaacagcacg tagccgagcg gcagcgccag cacgcgggcc gttacgaccg agcagagcag
     1441 ccagaccgcc ggcccgaccg cccagcccca gtcctcgaag aagtcgcgat ccagcgcgat
     1501 cccgaggatc gcgaacggga tcgccaccgc cacggcctgc accaacagcg acttccagaa
     1561 cagccgggcg ttcacaccgc ctgatactag actcgcgcgc gggggcggtt agctcagttg
     1621 gtagagcgcc acgcttacaa cgtggaggtc gcctgttcga gccaggcacc gcccatcagg
     1681 cccggactgg aatgcgggca gtctcccgag aatcacgccg tgaacccgat cactggcttg
     1741 gtcaccacca taggtgcgct gggaacaagc gacacctcgc tcgtgaggcg agcaccgctg
     1801 gaatccatcg aaccgcgtcg gatacggttg ccggatccag gtggatgcgc cagccaacga
     1861 ggccccctat cgcctgatcc acgacgtgag cttcgggcag ggcgtcgtcg tcgcggcgtt
     1921 cgtgaatctc tatggctgcg agatcggagc tggcacgcgt attggcccct tcgtggagat
     1981 ccagaagggc gcacgcgtcg ggacacggtg caaggtgcag agccatacgt tcatctgcga
     2041 aggagtcact atcggcgacg aggtgttcgt ggggcacggc gtcctcttcg tgaacgacaa
     2101 gcggccgcgc gcgaccaatg cggccggcga gctccaaggc gatgcagact gggagctgct
     2161 tgacacgtgc gtcgagagcg gcgcaacgat cgggtccggt gccgtgatcc tcggcgggat
     2221 tcgactcggg accggagcga tgatcggggc aggggcgctg gtggcgaagg atgtgccacc
     2281 gcatgccacc gtggcgggca atcccgcacg gcttctacac ggggtccccc gccatgacct
     2341 tgggtcggct tgatccgcgg gtgcgctcgc cgatgtccca gggctccggc cgcttgtagc
     2401 ccgcggccgc ctggacgctc gacggtcgcc acgtggcgcc gccgcgaacg gtgggcacgc
     2461 cctcctcgtt gagcatgtcc gcgatcgcct ggagggtgag ccccgcgccg cggagcctca
     2521 cgatgcgacg tctgagggtg gacgagatag tgccggtaac agggatcttc gaccgagccg
     2581 ctgctgtcat ctcagcgcgt ctttcgcgct cccagtcgct cattgagacg agcgcccgta
     2641 aaaccgcacg gccaaccgga tttctggtgt cgagggcggg gtcgagcgag accaaccgga
     2701 cgtcccgctc ctcgatggca accaggatcg aacccagttc tgcgatcgaa gggcagagcc
     2761 ggctcagctc cgtcgtgacc aggcagtcgg cttcgccccg gccgatgcgt tcgagcgctg
     2821 agatcagtga gggcctgccc caggcccgac tccttctcgg ctcgacgtct ctcagcaggg
     2881 cgacgagatc ccaccctcgc cgctggcaaa agctcgcgac gatcgcttcc tggcgcgcga
     2941 agtcggggtc gtcgatctgt gcggggctac ggacgctgcc gtagccgacg accgagacac
     3001 gtctgtctgt aagaggctgc ttgaaataca tccgttcctc ccggaaagct tgtggcccgc
     3061 gaccactgcg cgggcacccg gcgctggggt tgcgcgggcg gctagccggg gcgctcgggg
     3121 agcgcgagga acgggttgaa gcggtagctg tccagtgcca ggacgagcag cgaccaagcg
     3181 ccgaccaaga gcaaggcaag agccgatctc agatccatcg gggaggcggc actcgatcca
     3241 gtcgagccac cgacgccagc gccagccgag gcgccgggtg gtgtcgagtc cgactgccgg
     3301 acgtccgggc tgggggcggc aacggggata ccgtcccccg caggacgggt cgaatggacg
     3361 ctcgcggcca gtccggtgaa ggccacgcgg tcgcccgcga gcagcccgag cgcgaactgc
     3421 ggcagcgcgg acgcgcgctc ggcacgcggc gcgcttggtg agggagcggc tgcgtcactg
     3481 cggggtgccg tggcgggcgg ctgaggagcc attgggagga tcg
//
LOCUS       DASRJQ010000110         3023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_204335, whole genome shotgun sequence.
ACCESSION   DASRJQ010000110 DASRJQ010000000
VERSION     DASRJQ010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204335"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="VFB89_02525"
     CDS             complement(<1..719)
                     /locus_tag="VFB89_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HZM26206.1"
                     /translation="MKSHGIDPRTVGELRNSPWAEAPLRGRTVRDEVRSNLLVKLESG
                     EELFPGIVGYEDTILPQLVNALLARHHFILLGLRGQAKSRILRQMIELLDERIPIVAG
                     SEVNDDPFNPISKYARDRLGECGDATAIAWLHRSQRYVEKLATPDVTIADIIGDVDPI
                     KAARGGHLLSDELTMHFGLLPRANRGIFAINELPDLSGKVQVGLFNIMQEGDVQIKGY
                     PVRLPLDVLLTFTANPEDYTAR"
     gene            965..1153
                     /locus_tag="VFB89_02530"
     CDS             965..1153
                     /locus_tag="VFB89_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26207.1"
                     /translation="MKKILFLGAVLALAGCAGEKKAEAPASSEAPAAGAPAMAPADTG
                     MGTMQHDSTMARDTSKKM"
     gene            complement(1224..2213)
                     /locus_tag="VFB89_02535"
     CDS             complement(1224..2213)
                     /locus_tag="VFB89_02535"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26208.1"
                     /translation="MSPRELSYDRRRLERLRRGAVLVVGEPAVFWVEGPGALPCLQGL
                     LTNDLEKPGEHSLVYGALLTPKGAIVVDHWVFREPERFVLIAPINGRLPSVDLFQRQL
                     PPRLARVSDATGQVEVAWLVGGQAFAALARSSVKPIPDAAGRTVALEYPHGRLLLGLA
                     PEAAPFAAIAVGPRTAIRDFARLLDGAGVEAGTPEDAEAARILAGWPALGAEIDDRTL
                     PQEVRYDEVGGVSYTKGCYVGQETVARLHFRGHTNRELRGLVWREGAGIAGTEITASD
                     RQVGTIRSTLALDDRLLGLAPIRREVSVGDEVDAGGKKARVVALPFGAEDLDG"
     gene            complement(2200..2868)
                     /locus_tag="VFB89_02540"
     CDS             complement(2200..2868)
                     /locus_tag="VFB89_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HZM26209.1"
                     /translation="MQRAILFDFNGVIVDDEAQHCDALAAALAEYDISLDREAYYRDY
                     LGFDDRECFRFTFARHELPADSKALAKAIERKAAHYAAAIRNSMTLVPGAPEFVRAAA
                     DGGHALAIVSGALRREIELVLDTAGLREHFTTLIAAEDVSACKPDPQGYRRARASLGV
                     PREYCVVLEDSLPGLAAARAAGIRCAALATSHPADALGGADAVWPDLVGRKPADLPWA
                     DVAA"
BASE COUNT          567 a          998 c          951 g          507 t
ORIGIN      
        1 cgtgcggtat agtcctcagg attcgcggtg aatgtcagca gcacatccaa cggcagtcgc
       61 acggggtagc ctttgatctg gacgtcaccc tcctgcatga tgttgaacag gccgacctgg
      121 accttgccgc tcagatcggg cagctcgttg atcgcgaaga ttccgcggtt ggcgcgcggc
      181 agcagcccga agtgcatcgt gagctcgtcc gacaacagat ggccgccgcg agctgccttg
      241 atcggatcga catcgccgat gatgtccgca atagtcacgt ccggcgttgc gagcttctcg
      301 acgtaccgtt gcgaccgatg caaccacgcg atggcggttg cgtcgccgca ctcccccagg
      361 cggtcacgag cgtacttgga tatcggattg aacggatcgt cgttcacctc gctcccggcc
      421 acgatcggaa tccgctcgtc cagcagctcg atcatctgtc gcaggattcg gctcttggcc
      481 tggccccgca agccgagcag aatgaagtgg tgccgagcca ggagggcgtt gaccagctgg
      541 ggaaggatgg tgtcctcata gccgacaatg ccggggaata actcctcccc cgactcgagc
      601 ttcaccagca gatttgatcg gacttcgtcc cggacggtcc ggccacgaag gggcgcttcg
      661 gcccagggac tgttgcggag ctcccctacg gtacgggggt cgatgccatg cgatttcacg
      721 aagttacctc gaattgcggg cccctcggaa cgcggtgggg ccagtgattg ccggaggtgc
      781 cgatacggac cgaccggggc aaggccgctg gggtacagcg cgggaatcgt gcccgtaact
      841 cggcccccga ttgcggcttg acaaacggag attgtctctt accttcgccg cggtatgccc
      901 gacgacagtg gcggcatacg cgcagacatc tctcgagatt tccaaacccc tgaaggagta
      961 tgagatgaag aagattctct tcctgggcgc cgtgctggcc ctcgcgggtt gtgccggcga
     1021 gaagaaggcc gaagcccccg catcgtccga ggctccggcg gccggagcgc ccgccatggc
     1081 ccctgccgat accggtatgg ggacgatgca gcacgacagc acaatggcgc gggataccag
     1141 caagaagatg taagcgcgat cgccgaactg tcgtcgccgg ccccggggaa cctcgcttcc
     1201 cggggcttgg ccatttttgc gggctagcca tcgagatcct cggccccaaa cggcagcgcg
     1261 acgaccctgg ccttcttccc gccggcgtcc acctcatctc ccacggatac ctcccgccga
     1321 atcggtgcca ggcccaataa gcgatcgtcc agagcgagcg tcgaccgaat ggttccgact
     1381 tgacgatctg acgcggtgat ctcggtgccc gcgatgccgg caccctcgcg ccacacgagc
     1441 ccacgcagct cccgattggt gtgtcctcga aaatggagcc gcgcgacggt ctcctgtcct
     1501 acgtagcaac ccttggtgta agagacgcca cctacctcgt cgtaacgaac ttcctgcggc
     1561 agcgtgcgat catcgatctc cgcgccgagt gcaggccagc cggcgagtat ccgggcagct
     1621 tcagcgtcct cgggagtccc ggcctcgact cccgctccgt cgagcagccg agcgaagtcg
     1681 cggatcgcgg tgcgcggtcc taccgcgatc gcggcgaacg gcgctgcttc cggcgccagg
     1741 ccgagcagca agcgcccgtg aggatactcg agcgcgacgg tgcgaccggc cgcatccgga
     1801 atgggcttta cggatgagcg cgccagcgcg gcgaaagcct gcccccctac cagccaggct
     1861 acctcgacct gtcccgtggc gtcggagacg cgggccagcc tcggcgggag ctgtcgttga
     1921 aacagatcta ccgacgggag ccgaccgttg atcggtgcga ttagcacgaa tctctcgggc
     1981 tcgcggaaca cccagtggtc caccacgata gcacccttgg gtgtgagcag agcgccatag
     2041 accagactgt gctcgcccgg cttctcgaga tcgttggtca gaagcccctg aagacagggc
     2101 agggcgcccg gaccttcgac ccagaatacc gccggctctc caacaacgag cacggctccg
     2161 cggcgaagcc gttccagccg gcggcggtcg tagctgagct cacgcggcga catcagccca
     2221 cggcaaatcg gcaggtttgc gaccgaccag atccggccac accgcgtcgg caccgccgag
     2281 cgcatccgct ggatgggagg tggcgagagc cgcgcaccgg attccggccg cgcgagccgc
     2341 ggccaggccg ggcaacgagt cttccagcac aacgcagtat tctcgaggaa ctcccagcga
     2401 cgcacgggcg cggcgatagc cctgcgggtc gggcttgcaa gccgacacgt cttcagcggc
     2461 gatgagcgtc gtgaaatgct cccgcaaccc ggccgtgtcg aggaccaact cgatttctct
     2521 ccgtaacgcg cccgaaacga tggccagagc gtgacccccg tccgcagcgg cgcggacgaa
     2581 ctcgggtgcg cccggtacca gcgtcatcga attccgaatc gcagctgcat agtgggcggc
     2641 cttccgctcg atcgccttgg ccaatgcctt cgaatcggcc ggcaactcgt gtcgggcgaa
     2701 ggtgaagcgg aagcactccc ggtcgtcgaa gcccagataa tcgcgatagt aggcttctct
     2761 atcgagcgag atgtcgtact ctgccagcgc cgcggccagc gcatcgcagt gctgcgcttc
     2821 gtcgtcgacg atgacgccgt tgaagtcgaa gagaatggct cgctgcataa ggagcgacag
     2881 aaaaagcgta aacggtgaac ggtgaacggt aaacgggggt gtcgcctgcg cacagaccct
     2941 ctcgctgcta cctgttcacc gtttaccgtt aacgctcttg tcgtaaatcc cagcattatc
     3001 gtaggaccag tccaaaacct cca
//
LOCUS       DASRJQ010000111         7602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2744879, whole genome shotgun sequence.
ACCESSION   DASRJQ010000111 DASRJQ010000000
VERSION     DASRJQ010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7602
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2744879"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VFB89_02545"
     CDS             <1..419
                     /locus_tag="VFB89_02545"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZM26210.1"
                     /translation="PENILLADGHALVADFGLARAIGAADYQKLTETGIIVGTIFYMS
                     PEQLKEDRGLDQRADIYSLGCILFEMLTGAPPYTGTSLKDVALKILRAPVPSAQRLNP
                     AVSTAVDHAIAKSLARPLAERFASMQEFAAALPQPR"
     gene            complement(416..904)
                     /locus_tag="VFB89_02550"
     CDS             complement(416..904)
                     /locus_tag="VFB89_02550"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZM26211.1"
                     /translation="MLLDNRRSIPAIAAFAVLIACAPAKQSPPEPEPAGRAEMPASPE
                     PPTPAPTSDPYHPAPHDTVDAVVYDGWKQYFLQCSRCHGEDANGSSFGPSLLAALKPD
                     GSVPTQDEFVNVLTHGRRDKGMPSAATMGVDSSYFAGLYRYLKGRSDGTLRAGRPARR
                     TS"
     gene            complement(928..1806)
                     /locus_tag="VFB89_02555"
     CDS             complement(928..1806)
                     /locus_tag="VFB89_02555"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZM26212.1"
                     /translation="MPHLARKVLFAGPLVRLVDVRCREVSSTCSGEERADTHQLIVVR
                     AGVFVKHPAFASRQSLVAEPMHALLLNRHNPFRISHPARGGDDCTVLEISPDAVTEVA
                     AWLEERIRDRPQTPFLISHALFTAPMRIELHRIRSAAWRAADPMAGEERLLELLGQVL
                     AGGYRAHGRRKAPRREETRRAQRDLVERTRLILSSSAAKSWSLSELAREVASSPFHLT
                     RVFHATTGMPIHRYLRDLRLALALEQLGSARGRLSEVAVRSGFATHSHFSTAFRRAFG
                     VTPSLSQSPSGMSAGP"
     gene            1991..2452
                     /locus_tag="VFB89_02560"
     CDS             1991..2452
                     /locus_tag="VFB89_02560"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZM26213.1"
                     /translation="MRLVGLLPGGAAVIAGILLLGAARSHSAPADDVAIVAALDTKYQ
                     AAVEANDWATMDAILADDFVLVTGRGTVYTKADLLKEARNKTIIYEFQKDSSQTVRVW
                     GNTAVVTALLMEKGTVSGKPFEKRLWFSDTYVRTPAGWRYVFGQASLPLPD"
     gene            complement(2456..3553)
                     /locus_tag="VFB89_02565"
     CDS             complement(2456..3553)
                     /locus_tag="VFB89_02565"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HZM26214.1"
                     /translation="MRLIQSRLTVTLAVVLASGCASGMQESEPVRIRLESSKKAKPAA
                     RTFGPPATYTGRLPCADCPGIQLTLTLLSDSTFRLRQAYRERPTVVHQLGRWSVDSTR
                     TQLTLQGPAPSYRFQIVGADSLRLLDSQGSPVQSPLNYTLARAAKVDPIRDTMALRGS
                     YVYMADAGRFTECGSGSSYPVAQAGDNANLERAYGEARTEPGAPVLATFRGHFELRPA
                     MEGDKRLEYVVVDSVGQVQAGSACEEPKSNATLENTYWKLIQVGGQTARVAENVPEPH
                     FLLQPAQQQADGSTGCNQFHGPYQLAGDSVRIGPLASTLSACTDPEMNGQEAAFLDAL
                     SRARSWKVSGDTLTLADETGQLARFAAQYMK"
     gene            complement(3577..4026)
                     /locus_tag="VFB89_02570"
     CDS             complement(3577..4026)
                     /locus_tag="VFB89_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26215.1"
                     /translation="MGGEPYYYFVPYQQDIGAALQALRRREFEAGRYNPAMPFPSRYL
                     PLGPESPAPGRQHDSIEDALEDSDADGTRSILDIERVSDTPDFSVATRLSDESLISLY
                     GTTRPSRDQVEEEMAFLDDIERGYCVYVILYQGDTPSEILFAGYSYD"
     gene            complement(4061..4699)
                     /locus_tag="VFB89_02575"
     CDS             complement(4061..4699)
                     /locus_tag="VFB89_02575"
                     /inference="COORDINATES: protein motif:HMM:NF025879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HZM26216.1"
                     /translation="MALRGRDRGCHRSRRRGSRRGTRATFPAGDREVACPTCQPIPSA
                     WCAGRNARLGPERRRSRPRRIAGISVRTSNAAEMNPSHAQIPRLWHRFGSEHWPARLE
                     QLGASGPVHAVYCDYESDVSGPYRLLLGRGVEPAEPPPSDVEVITVPEGDYLVFSCVG
                     PMPGAVLEGWCRIWQFFAEPGAPRRAYTADFELYQSETASPDIWIAVLQTGS"
     gene            complement(4600..4968)
                     /locus_tag="VFB89_02580"
     CDS             complement(4600..4968)
                     /locus_tag="VFB89_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26217.1"
                     /translation="MNLRLIGSVLAGLLACGITLWPVPYEKVSLPENPAGSTWFLLGA
                     VAGLLAGYLLKQKKRVPVLAVAGGFVLAVLGRVAVETSRDPTSHNLWPFEVAIAGVIG
                     LGAAVVGVGLARLFQRATAK"
     gene            5032..5592
                     /locus_tag="VFB89_02585"
     CDS             5032..5592
                     /locus_tag="VFB89_02585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZM26218.1"
                     /translation="MPTEPDRLSPALENVLGNFSGVLRHVSSRFRFTGSDVDELVQEV
                     RIRLWRAHARDGEGSEQIEAIPASYLHRTALSAAIDLLRRRRARRADQMVPLEDEPGD
                     VPEPRGPEGDFEAAELTEQVERAIETIPASRRPVVRMHLMGHSRDEIASVLGWSDSKT
                     RNLLYRGLADVRERLVAQGVRWSSPT"
     gene            5589..6242
                     /locus_tag="VFB89_02590"
     CDS             5589..6242
                     /locus_tag="VFB89_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26219.1"
                     /translation="MSETTRLQQLYQRRVAARDTGDRSDCVSIDGILAIIQREGSEDE
                     RLAALDHVMACPDCHREYEWLMAVDEAATTAQPRARDIARARWWQRGVPLALAATVLL
                     AVGVLLIPKSKRQGPEPERGNESDIVPIAPVTNAQVRLPFTLAWHPVPGATRYVLEIQ
                     TGEGTIALADTTSDTTVTLADGSRLSAGATYRWWVREVTDGAAPRSSPFSSLRLTPP"
     gene            6388..6981
                     /locus_tag="VFB89_02595"
     CDS             6388..6981
                     /locus_tag="VFB89_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26220.1"
                     /translation="MTGVINDRTSDWEDRMLARPTTLGIFAAMALALPPSRAPFFTLG
                     AGGAVNLAVTSDEARFGMSPVDVEGAPLLTISLGATSAAGSLSLSISGDRLPAKGRYP
                     VRPWEDRTASRPQFEALFVAGSPDHPLGAFRGEFGWVTITEVEPGRISGVFEVEGRGF
                     VASDVNDEDRWVSVRGRFEAQGDSTITSGQALSASMQ"
     gene            complement(6989..>7602)
                     /locus_tag="VFB89_02600"
     CDS             complement(6989..>7602)
                     /locus_tag="VFB89_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZM26221.1"
                     /translation="IEHMFEPFFTTKPVGEGTGLGLSTAYGIIRQTGGYLGFQSAPGR
                     GTVFSIFLPLTTDPLSLPGPEEFASIAAGREVVLVVEDETEVRRIAARALRAGGYDVL
                     EAADGEAALDLIRSRGDDIRLVLTDLAMPRMGGFDLAREVASIAPRIQILFMTGYTSS
                     EAMRRSALRLGYAMLEKPFTPEILAARVRAALDTLAGAAPTAV"
BASE COUNT         1319 a         2444 c         2550 g         1289 t
ORIGIN      
        1 agccggaaaa catcctgctc gccgatggac atgccctggt agccgacttc gggctggccc
       61 gggccatcgg cgcagccgac taccagaagc taaccgagac gggcatcatc gtcggcacca
      121 ttttctacat gagcccggag cagctcaagg aagaccgggg cctggaccag cgggccgaca
      181 tctacagtct ggggtgcatc ctgttcgaga tgctgacggg agcgccgccc tacaccggaa
      241 cgtcgctcaa ggacgtggca ctcaagattc tgcgggcgcc cgtcccctcg gcacaacgat
      301 tgaaccccgc cgtatcgact gcagtggatc acgcgatcgc gaaatcgctg gcgagaccgt
      361 tggccgaacg gttcgcgagc atgcaggagt tcgctgccgc gttgccgcag ccgcgctagc
      421 tcgtccgtct cgcgggccgc ccggcccgga gcgtcccgtc gcttcgccct ttcagatacc
      481 ggtagagtcc cgcgaaatag gacgagtcga cgcccatggt cgcggcggag ggcattccct
      541 tatcgcgacg gccgtgcgtg agcacgttca cgaactcgtc ctgagtggga accgaaccat
      601 ccggcttgag cgcggccagg aggctcggcc cgaacgagct accgttggcg tcctcgccgt
      661 ggcaacggga gcattggagg aagtactgct tccagccatc gtagaccacc gcatccaccg
      721 tatcgtgcgg agcggggtgg taagggtcgg atgtgggcgc cggtgtgggc ggctctggac
      781 tcgccggcat ctccgcgcgc ccagccggct ccggctcggg cggcgactgc ttggccggtg
      841 cgcaggcgat caggacagcg aaggccgcga ttgccggtat cgaccggcgg ttgtcgagga
      901 gcatggtcga atccctcggc gccaaggtta cggccccgcg ctcattccgc tagggctctg
      961 agaaagtgag ggagtcaccc cgaacgcacg ccggaaggca gtgctgaagt gactgtgcgt
     1021 tgcgaatccg cttctaacag caacttcgct cagtcgcccc ctcgcgctgc cgagctgctc
     1081 caaagccagc gcgagcctga gatcccgcag gtatcgatga atcggcatac ccgtggtagc
     1141 gtggaacacg cgggtcagat gaaagggtga gctggcgacc tcccgagcca actccgacag
     1201 cgaccacgac ttggccgccg agctggagag gatgaggcga gttcgttcca ccaggtctct
     1261 ttgcgcccgg cgcgtctcct cgcgccgagg ggctttgcgg cgaccgtggg cccgatatcc
     1321 gccggccagt acttggccga gcagctcgag cagccgctcc tcccccgcca tcgggtctgc
     1381 agcgcgccag gcggccgagc gaattcggtg cagctcgatc cgcatcgggg cggtgaagag
     1441 tgcatgcgag atgaggaacg gcgtctgggg ccgatcccgg atgcgctcct ccagccacgc
     1501 ggctacttca gttaccgcat cgggcgagat ctccaggacg gtgcagtcat caccgccgcg
     1561 tgcggggtgg ctgatgcgga acgggttgtg ccggttgagc agcaacgcat gcatcggctc
     1621 ggcgaccaga ctctgccgcg acgcaaacgc cgggtgcttc acgaaaactc ccgcccggac
     1681 aacgatcagc tgatgcgtgt cggctcgctc ttcgccggaa caggtcgagg agacctcccg
     1741 gcagcgaacg tccacgagtc ggaccagcgg cccggcaaac aggaccttac gcgcaagatg
     1801 aggcatgggg cggtattagt cggcgacctg cttcgaagat accggcgccc tggcaggggg
     1861 caaagaatcg gggccggcgt tttgagcaag attgcgaaag cgcggcgtca acctgaatgc
     1921 gagattcggg tgcgtctaga gcgcctgcgc cgagaccggc gccggccggt cttctcctgt
     1981 gagggtaatt atgcgattgg tcggacttct ccctggcggt gccgccgtca tcgctggaat
     2041 cctgctgctc ggcgccgcgc gcagccactc ggcgccggcg gacgacgtgg ccatcgttgc
     2101 cgcgctcgat accaagtatc aggcagcggt cgaggcaaat gactgggcca cgatggacgc
     2161 catactggcc gacgactttg tcctggtgac cgggcgcggg acggtgtaca cgaaggcaga
     2221 cctgctcaag gaggcccgca acaagacgat catttatgag tttcagaagg actcgagcca
     2281 gacggtacgg gtgtggggca acactgccgt ggtaactgcg ctgctcatgg agaagggtac
     2341 cgtctcgggc aagccgttcg agaagcggct ctggttcagc gatacctacg tgcgaacgcc
     2401 ggcgggctgg cggtacgtct tcggccaggc gtctctcccg cttccggatt gaatcctact
     2461 tcatgtactg tgcggcaaac cgggccagct ggcccgtttc gtccgccaat gtgagtgtat
     2521 cgccggacac cttccagctg cgcgctcgtg agagcgcatc gaggaaggcg gcttcctgcc
     2581 cgttcatttc aggatcggtg caggcgctca gtgtggatgc gagagggccg atgcgcaccg
     2641 aatcgccggc gagctgatag ggtccgtgga actggttgca gccggtactg ccgtccgctt
     2701 gctgctgagc gggctggagc agaaagtgcg gctcggggac gttctcggcg actcgtgctg
     2761 tctggccgcc cacctggatg agcttccagt acgtgttttc cagcgtcgcg ttcgacttcg
     2821 gctcctcgca ggcactgccc gcctgaacct gcccgacgct gtcgacgacg acgtactcca
     2881 gccgcttgtc cccctccatc gcgggccgca gctcgaaatg gccgcggaag gtagcgagca
     2941 cgggagcgcc gggctcggtg cgggcctcgc cataggcacg ctcgaggttc gcgttgtcgc
     3001 ccgcctgcgc cacgggatag ctgctgccgc tgccgcactc ggtgaaccga ccggcgtccg
     3061 ccatgtagac gtaactaccc cgcagcgcca tcgtgtcacg gatcgggtcc accttcgctg
     3121 cccgggcaag cgtgtaattc aacggcgact gcaccgggct gccctgactg tcgagtagac
     3181 gaagcgagtc cgcgccgacg atctggaagc gatagctggg agccggaccc tgaagagtga
     3241 gctgggtgcg cgtcgaatcg accgaccagc ggcccagctg atggacaaca gtcggacgct
     3301 cccggtaagc ctgacgaagc cggaacgtgc tgtcggacag cagagtgagc gtcagttgga
     3361 tgccggggca gtcggcacag gggagccggc cggtataggt agcgggcggc ccaaacgtcc
     3421 gcgccgccgg tttggccttc ttgctcgact cgagcctgat tctcaccggc tcgctttcct
     3481 gcatcccgga cgcgcacccc gacgcgagca cgacggcgag cgtgaccgtc agcctgcttt
     3541 ggatgagccg catatcgctc ctgttagtgc acccgactaa tcataggaat agccggcgaa
     3601 gagaatctcg gagggcgtgt cgccctggta caggattacg taaacgcagt agccccgttc
     3661 gatgtcgtcg aggaaggcca tctcttcttc cacctggtcg cggctgggcc gagtcgtgcc
     3721 atacagagag atcagagact cgtccgataa tctggtggcg acgctgaaat cgggagtatc
     3781 ggatacccgc tcgatgtcca ggatcgatcg ggtgccgtcg gcgtccgagt cctcgagggc
     3841 gtcttcgatt gaatcgtgtt gccgacccgg cgccggcgac tcggggccga gtggaaggta
     3901 ccgggacgga aagggcatgg cgggattgta gcgtcccgcc tcgaattcgc gccggcgcag
     3961 cgcctgcaat gcggcaccta tgtcctgctg gtacggcaca aaataatagt acggctctcc
     4021 tcccacgacg cgtcccccgc gctaagacga caaagcttcc tcatgatccc gtttgaagca
     4081 cggcaatcca gatgtcgggc gatgcagttt cgctctgata tagctcgaag tccgcggtgt
     4141 aggcccggcg cggcgcaccg ggctccgcaa agaactgcca gatccggcac cagccttcca
     4201 gaacggcccc tggcatcggc ccgacgcagg agaacaccag gtaatctccc tccggaacag
     4261 taatgacttc gacatccgaa ggtggcggct cggcgggctc gacgccgcgg ccgagcagca
     4321 gccggtaggg tcccgagaca tcactttcgt agtcgcagta gaccgcgtgc accggaccgc
     4381 tggcgcccag ctgttccagc cgggccggcc agtgttcgct cccaaagcgg tgccacaagc
     4441 ggggaatttg ggcgtggctg ggattcatct cggccgcgtt gctggtgcgt accgagatgc
     4501 cggcaattcg cctggggcgg gaccggcgac gctcaggtcc caggcgggcg ttcctcccgg
     4561 cgcaccacgc tgacgggatg ggctggcagg tcgggcatgc tacttcgcgg tcgcccgctg
     4621 gaaaagtcgc gcgagtccca cgccgactac cgcggcgccg agaccgatga cacccgcgat
     4681 cgcgacctcg aagggccaga ggttgtgaga ggtcggatcg cgggaggtct cgaccgcgac
     4741 tcgacccagt actgccaaga caaacccgcc cgcgacggcg agcaccggaa cgcgcttctt
     4801 ctgtttgagc agatatccgg cgagcagtcc agcgactgcg ccgagcagaa accaggtcga
     4861 gcccgccggg ttttcgggga ggctgacctt ctcgtagggc accggccaga gggtgatgcc
     4921 acaagccagg agccccgcca gcaccgaccc gatgaggcgc aggttcatgc agacaaaata
     4981 cctgaaacgc cttcgattgc cgccccggcc gcgtcggcct actttgtccc catgccaacc
     5041 gagccggatc ggctctcacc agcgctggag aacgtgctcg gcaatttctc cggcgttctt
     5101 cggcatgtct cttcgcgatt tcgctttacg gggtcggacg ttgacgagct ggtccaggag
     5161 gtccggatcc ggctttggcg agcccatgcc cgggacgggg aggggagtga gcagatcgag
     5221 gccatccctg cgtcctatct ccataggacg gccctgtccg ctgcgatcga tcttctccga
     5281 cggcgccgag cccggcgggc cgatcagatg gttcccctcg aagatgagcc gggcgacgtg
     5341 cctgagccga gaggtccgga aggggacttc gaggcggccg agctgaccga gcaggtagag
     5401 cgggcgatcg agacaatccc cgcttcccgc agaccggttg tccggatgca cctcatgggc
     5461 cattcgcgag acgagatcgc cagcgtcctt ggatggtcgg acagcaagac tagaaacctg
     5521 ctgtaccggg gcttagcgga tgtgcgcgag cgcctggtgg cacagggggt acgatggagc
     5581 agcccgactt gagtgagact acccgtctcc agcaattgta ccagcgacgc gtcgcggccc
     5641 gggataccgg cgaccggtcg gattgcgttt caatcgacgg catcctcgcc atcatccagc
     5701 gcgaaggcag tgaggacgag cggcttgcgg cactcgacca cgtcatggcc tgtcctgact
     5761 gccaccggga atacgagtgg ctcatggcgg tcgacgaggc ggccaccacg gcccaaccgc
     5821 gggcccgcga tatcgcccga gcccggtggt ggcagcgggg cgtcccgctt gcgctcgccg
     5881 cgacggtgct gctggccgtc ggggttctgc tgatcccgaa gtcgaagcgg caagggcccg
     5941 agcccgagcg agggaacgaa agcgatatcg tcccgatcgc ccccgtcact aatgcgcagg
     6001 tcaggctgcc attcacgctg gcctggcatc cggtgcctgg agcaactcgg tacgtgctcg
     6061 aaattcagac gggcgaggga acgatcgccc tggcggacac cacgtcggat accacggtga
     6121 cgcttgcgga tggaagccgg ctgtccgccg gagccaccta tcgctggtgg gttcgtgagg
     6181 tcaccgacgg tgccgcaccc cgcagctccc cattcagcag tcttcggctg actcctccgt
     6241 gacgtggggc ggcgtttggc cgcccctctt catcccgtgc gatcatcggc tccgtgagaa
     6301 tcggcgcccc caggctgcgt acacacctct gaagcggcga tggagcgccg acctttacat
     6361 cacgttcttt cgctgaggac taccaccgtg accggcgtga tcaacgaccg cacttctgac
     6421 tgggaggatc gcatgcttgc tcgccccacg acgctcggca tcttcgccgc gatggcgctg
     6481 gcattgccgc cgagccgggc tccgttcttc acgcttggcg cggggggcgc ggtcaacctg
     6541 gccgtcacca gcgacgaggc gcggttcggg atgagccccg ttgatgtgga gggggctccg
     6601 ttgctcacca tctcactcgg cgctacaagc gcggccggct ccctctcgct ctcgatctcg
     6661 ggcgatcggc tgcccgccaa gggcaggtac ccggtgcggc cctgggaaga ccgcacggca
     6721 tcgcggccgc agttcgaggc gctcttcgta gcggggtcac ccgatcaccc gctgggtgca
     6781 ttccggggtg agttcggctg ggtgacgatc accgaagtcg agccgggacg gatctcgggt
     6841 gtgttcgaag tggaaggccg cggtttcgtt gcctcggacg tgaacgacga ggatcggtgg
     6901 gtgtcggtgc gcgggcggtt cgaggcgcag ggggacagca ccattacgtc gggccaggcg
     6961 ctgtccgcat cgatgcagta gccgatcgct ataccgcggt cggagcagcc ccggcgaggg
     7021 tgtccagtgc agctcggacc ctcgccgcga ggatctccgg tgtgaacggc ttttcgagca
     7081 tggcataacc cagtcgaagc gcgctccgcc gcatggcttc cgagctggtg tagccggtca
     7141 tgaagaggat ctggattcgt ggggcaatgg aagccacctc gcgcgccaga tcgaagccac
     7201 ccattcgggg catcgcaagg tcggtcagca ccaaccgaat atcgtcccct ctcgagcgaa
     7261 tcaggtcgag tgcagcctcg ccatcggccg cttccagcac gtcgtagcct cccgcacgaa
     7321 gcgctcgggc ggcaatgcgc cgtacttcgg tttcgtcctc caccacgagc acgacttcgc
     7381 gccccgctgc tatcgaggcg aactcctccg ggcctggcag gctgaggggg tccgtcgtca
     7441 gcgggaggaa aatgctgaag actgtacccc ggcccggcgc gctctggaat ccgaggtagc
     7501 cgccggtctg gcggatgatg ccgtaggccg tcgaaaggcc gagcccggtg ccttcaccga
     7561 ctggcttggt cgtgaagaac ggctcgaaca tgtgctcaat cg
//
LOCUS       DASRJQ010000112         5258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_4153300, whole genome shotgun sequence.
ACCESSION   DASRJQ010000112 DASRJQ010000000
VERSION     DASRJQ010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4153300"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VFB89_02605"
     CDS             <1..807
                     /locus_tag="VFB89_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26222.1"
                     /translation="LADSLLRALPHPTPEQAENIAGLAALTGRPILTANLLELTASRG
                     PLDYSGEPVRVAPPLTQAALRLRAYASIGAPVDSLRAGILRTGKLIESYTESSRVAEL
                     RELLLERSLALAFPVLGKAAIPAGPAQHYLLRMEGALAKGDTVTYRKLLAEIRAARQP
                     FRPGDYSIEGTYAEAATQLMARDTADAEHLLDLSLNALPTLGTGLVTEVPQAAAITRA
                     MALRAKLAAHRRDQPTAARWAAGAATLWGSAEPALQSNLERMRRIMKGAE"
     gene            852..1550
                     /locus_tag="VFB89_02610"
     CDS             852..1550
                     /locus_tag="VFB89_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26223.1"
                     /translation="MPSGTRMRPFWWFWIVVLILIVIIYLMMPGTHPPPFVRIGEATP
                     VELAATCKDPKLHFMRFQASEADYDTAVRVRITPEFFSFKNKPEDLERGRVVALVQVL
                     RGTDSVPYGRKKLPYGLKDTIPACMFISGTHPNKLTTTIISREGERLYTPDSTWVIEK
                     THLLAEAHWQEDSIPRGATRLGFDLTPSALFAESPTRGGLSTAATRLVKRAFKTYGQS
                     SCTNHSCCVSSGGH"
     gene            1640..2356
                     /locus_tag="VFB89_02615"
     CDS             1640..2356
                     /locus_tag="VFB89_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26224.1"
                     /translation="MASATCRSGLWTAAELVVVALAVGCGPGTPAPPFKSLATADSAQ
                     IQAACDSGHFDLRYARAAEVAPGVTAVIIPESLSYRNKKEDYAPPGRVVAKIQLLHSA
                     RSDQFKAGDNPACVYLYGKFKDSLFMAVISQGKVLEDSIPTRARFGLHLFHPEAHWEI
                     GDTTYRAARAPAFAPQELYAEARDEDARTGAYLRAVQGAVRAYSQTSCTRTSCCAPGS
                     GPKTVLPVKAPSPGATPGPQ"
     gene            complement(2358..2780)
                     /locus_tag="VFB89_02620"
     CDS             complement(2358..2780)
                     /locus_tag="VFB89_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26225.1"
                     /translation="MQPYLWFLGMVLFSFVNHVTGLMGMPRRIFTDTYNGAPQAQMWE
                     GLTSVSALGGVVLFASAGCFVLVMIGTLLAGPRKPQPAIEWAEPLGRPERGYPVAIWD
                     KLGLWFAVAVALAAIAYVIPLWDLLHLTRYGSLGFKPF"
     gene            complement(2799..3278)
                     /locus_tag="VFB89_02625"
     CDS             complement(2799..3278)
                     /locus_tag="VFB89_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HZM26226.1"
                     /translation="MVAELFSEPLARMTFALFLIFSTPVGFHHQFQDPGIPAGWKLFH
                     TFNSMIISFPSLLTAFTVVASLEVAGRLRGGTGLFGWIRALPWGNPTVSSALLAGLLF
                     MVGGFGGSVNAAYALNSVVHNTAWIHAHFHLTVGSAVALTFMGTAYYLVPKLSGRDL"
     gene            3330..3479
                     /locus_tag="VFB89_02630"
     CDS             3330..3479
                     /locus_tag="VFB89_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26227.1"
                     /translation="MTKLLRALHFWCAEVDEAIRTVEQDELGAEATRDFDSLLEPGVG
                     ALTTA"
     gene            complement(3519..4946)
                     /locus_tag="VFB89_02635"
     CDS             complement(3519..4946)
                     /locus_tag="VFB89_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26228.1"
                     /translation="MKWSGRWLLGGLMLAAVPLAARVIIEGFDHPQHQSLFVSCTTCH
                     QGAATAGAALWPKPADCATCHDGTVQKRVDWTPRTGPPPSNLRFDHGTHANAVRKRAG
                     ADSTLDCAACHSARGAPWMTVSRAVVQNCLACHGIAASHLDAPDSACATCHVPLWQAP
                     AVPEARLASWSAPASHQAPDFAAGGHGKLAKGESCATCHARDYCATCHVNAPEEPTIQ
                     ALAADRRSLAIRVGTIAEPASHRRPDFLKRHGKDLGGKAAVSCTVCHTQESCSACHLA
                     PPSRLQLLPVAGPGRAAGARIERYRPASHGIDFSDAHKAPASADPQTCAGCHTREQCI
                     ECHRPNAAAQGGYHPRDFLSRHPVAAYSQEFSCGSCHNTSQFCQTCHQQSGIVATATL
                     GSKANYHDAKNSFLLGHGQAARQSLESCVSCHAERDCLTCHAAPAVGGRGFDPHGPGF
                     DANRLRSKNSQMCTACHGNVIPTAP"
     gene            complement(4943..>5258)
                     /locus_tag="VFB89_02640"
     CDS             complement(4943..>5258)
                     /locus_tag="VFB89_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26229.1"
                     /translation="TPTTRLSLRGYGATLDRPLELRFDDTDVRWAGLDAELRLQRDLS
                     FGLGGMYVYENHHRPDASAFDWDQVRITAHVTYVLGSSGADRLGLPKAVERMPSVVGT
                     GR"
BASE COUNT          995 a         1705 c         1681 g          877 t
ORIGIN      
        1 ctcgcagact ccctgctgcg cgctcttccc caccccactc ccgaacaagc cgagaacatc
       61 gctggcctgg cagctctgac cggccgcccg attctcacgg cgaatctcct cgagttgact
      121 gcgtcgcgcg ggccactgga ttactctggc gagccggtgc gagtggctcc gccgctgacc
      181 caggctgcgc tacggctccg agcatatgca tcgattggcg cgccggtcga cagtctccgc
      241 gccggcattc tgcgcaccgg gaagctgatc gagagctaca ccgaatcgag ccgcgtggct
      301 gagctgcgag agctgctgct cgagcgatca cttgccctcg cgttcccggt gctgggaaag
      361 gccgcaatcc ctgcagggcc cgcgcagcac tacctgcttc ggatggaggg ggcgctcgcg
      421 aagggtgata ccgtcaccta tcgcaagctg ctcgctgaga tccgagctgc gcgccagccc
      481 ttccggcccg gggactattc catagaaggc acttacgcag aagcagcaac tcagctgatg
      541 gcccgcgaca cggcggacgc ggagcacctg ctcgatctct cgctcaacgc gcttccaaca
      601 ctcggcaccg ggctggtgac agaggttccg caggctgcgg cgatcactcg cgcgatggcc
      661 ctgcgcgcta aactcgccgc acaccgccgc gatcagccga cagcggcccg ctgggccgcc
      721 ggtgctgcaa cgctctgggg atcggcagag ccggcattgc agtccaacct cgagcgcatg
      781 cgccgaatca tgaagggcgc agagtagtca tagagggtcg cagctcatgc cactcgctcc
      841 aggaggtcgc aatgccaagc gggacgagga tgcgtccgtt ctggtggttt tggatcgttg
      901 tcctgattct gatcgtcatc atttacctca tgatgccggg tacgcatcca ccaccgttcg
      961 tccgaattgg cgaagccact ccggtcgagc tggcggccac gtgcaaggac ccgaagcttc
     1021 atttcatgcg gttccaggcc agcgaggctg attacgacac ggccgtgcgt gttcggatca
     1081 ctccggagtt cttcagcttc aagaacaagc cggaggacct cgaacggggt cgcgtggtgg
     1141 cgctggtaca ggtccttcgg gggaccgact ctgtgcctta cggacgcaag aagctgccgt
     1201 acgggctcaa ggacacaatt ccggcctgca tgttcatcag tggcacacac ccaaacaagc
     1261 tgaccaccac gatcatttcg agagaaggtg agcggctata cacccccgac tcgacctggg
     1321 tcatcgagaa gacccatttg ctggcggaag cgcactggca ggaagacagc atccccagag
     1381 gtgcaacgcg gctgggcttc gacctgacgc cgtctgcttt gttcgccgag agtccgacaa
     1441 ggggagggct atcaactgcc gccactcgcc tggtgaagcg ggcgttcaag acctacggtc
     1501 aatccagttg caccaatcac agttgctgtg tctcgagcgg cggccactga ctcacatctc
     1561 gagcaggtgg cgcatcgagc gagaagcgtt gcactgatcc caagtttccg accacgtcac
     1621 gtcgttcctg gaggcagcta tggcaagtgc gacatgcaga agcgggctgt ggacagcggc
     1681 cgagcttgtc gtggtggcac tcgcggtggg ctgcggcccg ggcacgccgg cacccccgtt
     1741 caagagccta gctacagccg acagcgctca gattcaggcg gcttgcgata gcggccactt
     1801 cgaccttcgg tacgcccgcg cggcagaggt cgcccctggg gtgacggccg tgatcatccc
     1861 cgagagcctg agttaccgaa acaagaaaga ggactacgcg ccgcccggac gagtagtcgc
     1921 caaaatccag ctgctccact ccgcaaggtc tgatcagttt aaagcggggg ataatccggc
     1981 gtgcgtgtac ctctatggga agttcaagga ctcgctgttc atggcggtta tctcgcaggg
     2041 gaaggtactg gaggattcga ttccgacgcg cgcccggttt gggctccatc tattccaccc
     2101 tgaagcccac tgggaaatcg gcgacacaac ctaccgcgca gcccgcgcac ccgccttcgc
     2161 accgcaggaa ctgtacgctg aggctcgcga cgaggatgcg cggacaggag cgtatctccg
     2221 agcagtgcaa ggggcggtca gggcctactc tcaaacctca tgtaccagaa ccagctgctg
     2281 cgctccgggc agcggaccca aaaccgtctt gccggtgaag gctccgagcc ccggcgcgac
     2341 gccgggccct cagtagatca gaatggtttg aagcccagac tgccgtaacg cgtgaggtgc
     2401 aacagatccc agagcggtat cacataggca atcgcagcga gggcgaccgc gaccgcgaac
     2461 cagagcccaa gcttgtccca gatcgcgacc ggatagcctc tctcaggcct gccgagcggc
     2521 tctgcccatt cgatagccgg ctgcggcttg cgtgggccgg ccagcagggt tccgatcatt
     2581 accagcacga agcatcccgc gctggcaaac agcaccacgc cacccaacgc cgagacgctc
     2641 gtaagacctt cccacatctg cgcctgcggc gcgccgttgt acgtatccgt gaaaatccgg
     2701 cgcggcatcc ccatgagccc ggttacgtga ttcacgaacg agaagagcac catgcccagg
     2761 aaccagaggt acggctgcac cttggcaagg agcccgagct agagatcgcg tccgctcagt
     2821 ttgggaacca ggtaatacgc ggtgcccatg aaggtgagcg ccacggcgct ccccacggtg
     2881 agatggaagt gggcatgaat ccaggccgtg ttgtgaacca ccgagttgag cgcgtaggcc
     2941 gcgttgaccg agccgccgaa cccaccaacc atgaagagca ggccggcgag cagcgcgctc
     3001 gaaaccgtcg gattccccca cggcagcgcg cgaatccatc cgaagaggcc cgtcccgccc
     3061 cggagtcgcc cggctacttc gagcgaagcc accacggtaa acgccgtgag caggctggga
     3121 aacgaaatga tcatcgagtt gaaggtatgg aacaacttcc atccggccgg aattcctggg
     3181 tcttggaact gatgatggaa gccgacgggc gtggagaaaa tgaggaacag cgcaaacgtc
     3241 atccgcgcaa ggggctcgga aaagagctct gccaccacct ctgcgagctc gggaccatgc
     3301 cgcagtgcgg gcccgcattc gcggatgccg tgaccaagct gcttcgcgcc ctgcatttct
     3361 ggtgtgccga ggtggatgaa gccatcagga cggtagaaca ggacgagctc ggggccgagg
     3421 cgacacgtga cttcgacagt ttgctcgaac ccggtgtcgg agccctcacc accgcctgac
     3481 tggaaccgcg cggctgcctg aacgactttc caccggcctt acggtgccgt ggggatcacg
     3541 ttgccgtgac aggccgtgca catctgagaa ttcttgctgc ggaggcgatt ggcgtcgaag
     3601 ccggggccgt gcgggtcgaa gcctcggcca cccacggctg gggctgcatg acaggtgaga
     3661 caatctcgct cagcgtggca ggagacgcag ctctccaggc tctgccgtgc tgcctgtccg
     3721 tgacccagga gaaacgaatt cttggcgtca tggtaattcg ccttggatcc gagcgtagcg
     3781 gtcgccacga tgccggactg ctggtggcag gtctggcaaa attgactcgt gttgtggcaa
     3841 ctgccgcagg aaaattcctg gctgtaggca gctaccgggt gacgcgagag aaaatcgcgc
     3901 gggtggtatc cgccctgtgc ggcagcattg ggccgatgac actcgatgca ttgttcgcga
     3961 gtgtggcatc cggcacaggt ctgcgggtcc gccgaggccg gggccttgtg agcgtcagag
     4021 aagtcgatcc cgtgcgaagc cggtcggtag cgctcgatac gggcgccggc cgcacggccg
     4081 ggcccggcaa ccggaagcag ctgcagccga ctggggggcg ccaggtgaca cgccgaacag
     4141 ctttcctgcg tgtggcagac cgtgcagctc acagcggcct tgccgccgag atcctttccg
     4201 tgtcgcttca gaaaatcggg ccgccggtgt gaggcaggct ccgcaatcgt gcccacacga
     4261 attgccagcg accgccggtc tgccgccaaa gcctggatag tcggctcttc cggcgcgttg
     4321 acgtggcagg tggcgcagta atcccgggcg tggcaggtcg cgcagctttc ccccttcgcc
     4381 agctttccgt gaccaccggc ggcaaaatcc ggagcctggt gcgaggctgg cgcgctccag
     4441 gacgcgagtc tcgcttccgg caccgctggc gcctgccaga gcggaacgtg gcatgtagcg
     4501 caggcgctgt ccggcgcatc gagatgcgaa gccgcgatgc cgtgacaggc gaggcagttc
     4561 tgcaccacag cgcgacttac cgtcatccat ggcgcgccgc gcgcggagtg gcaggccgcg
     4621 caatcaagcg tcgagtccgc tccggcgcgc ttccgcaccg cgttcgcatg tgttccgtga
     4681 tcaaagcgga ggttgctggg cggtgggccg gttcggggcg tccagtccac ccgcttctgc
     4741 accgttccgt cgtgacaagt ggcgcagtcc gccggcttgg gccagagcgc cgcgccggcc
     4801 gttgcggctc cctggtgaca ggtcgtgcag gacacgaaca gcgactgatg ctgtgggtga
     4861 tcgaagccct cgataatgac cctcgccgcc agcggaacgg ccgcgagcat cagtccgccg
     4921 agcagccagc ggccgctcca tttcatcggc ccgtgcccac cacgctcggc atcctctcta
     4981 cggccttcgg cagccccagg cggtcggcac cggacgatcc aagcacgtag gtcacatgcg
     5041 ccgtgattcg aacctgatcc cagtcgaagg ctgacgcatc cggccgatgg tggttctcgt
     5101 acacgtacat cccgcccagg ccgaagctga gatcgcgctg gagcctgagt tccgcatcca
     5161 ggccggccca cctcacatcg gtatcatcga agcgcagctc gagcggacgg tcgagtgtcg
     5221 ccccataccc gcgcaacgag agccgggtcg tgggcgtc
//
LOCUS       DASRJQ010000113         2700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3305725, whole genome shotgun sequence.
ACCESSION   DASRJQ010000113 DASRJQ010000000
VERSION     DASRJQ010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3305725"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..641)
                     /locus_tag="VFB89_02645"
     CDS             complement(39..641)
                     /locus_tag="VFB89_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HZM26230.1"
                     /translation="MSLVNFAAREITCKIVYYGPGRSGKTTNLQYIFGRVPESRRGRM
                     VSLATHTDRTLFFDFLPLDLGSISGFATKFQLYTVPGQSYYASTRKLVLQGADGVVFV
                     ADSQARRLDDNLESLQDLQTNLLTQNIDIRQLPVVFQYNKQDLPGDLILSPPELDDAL
                     NFRAVQSFTADALHGGGVFETLKGISEQVLRRLAAGEPAT"
     gene            complement(646..1107)
                     /locus_tag="VFB89_02650"
     CDS             complement(646..1107)
                     /locus_tag="VFB89_02650"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HZM26231.1"
                     /translation="MRSVEPWVTEPLERFLAESSARLTLLMTSAGQVIAQHGFTRSVD
                     VMTAAALGAAIMASTGEIAKLVGERSFLSLVHQGARQHVYLAGFDTPRGRWIGLVVFD
                     AESTVGLVQLFFDRLISDLAAAAPRQAPEQQVLPENFERELNASLRALFGR"
     gene            complement(1107..1454)
                     /locus_tag="VFB89_02655"
     CDS             complement(1107..1454)
                     /locus_tag="VFB89_02655"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HZM26232.1"
                     /translation="MSNPVELLERLSTVRGLRGSVLVSAADGLIVAESLMTGVDGRAV
                     AALAASLMNRLRRATECAGRKEPVFLQLQAQSGSVLATPCGAELVLVAVAGPDANVGL
                     ARLEMLEAAGRLV"
     gene            complement(1451..2377)
                     /locus_tag="VFB89_02660"
     CDS             complement(1451..2377)
                     /locus_tag="VFB89_02660"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HZM26233.1"
                     /translation="MSDDIRGMTAELAADPDSLTFLDLGEALRRRGQLDAAFKVARTG
                     LSRYPELAKAHDLFARILCDRGEPDRAFESWVTALQLDAGLVSAHKGLGFLYYQAGDL
                     ASAEKHLAYSTQVDPDDSGTAAALERVRADAAASRAPQAAATAPEAVTAAQSDPGTDA
                     AAPGDASLPTIAATGGLAFSFDEGARSPVQEAVFSGLDGGADGLLLVDANGLRLGGGL
                     RNPDGVDVADSVAALLAGVSKEAARTARLLTLGDWRGLSVECGDGNLYLSAPSESTLL
                     LTVRGLDVPMGRVARFAERASVAARSWLERAE"
     gene            complement(2370..>2700)
                     /locus_tag="VFB89_02665"
     CDS             complement(2370..>2700)
                     /locus_tag="VFB89_02665"
                     /inference="COORDINATES: protein motif:HMM:NF024574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26234.1"
                     /translation="LAAVDLVAADPERPDGYVALARTLIRLARWEEAEDSLIQALHRD
                     PTLGAPRRLLALAQVAQGRFADAIESCDRWSRAGNQPGEREHANAVARLREASETLAH
                     VLGERDV"
BASE COUNT          477 a          915 c          901 g          407 t
ORIGIN      
        1 catcgtgtcg aaccggaagt aggggttgag cggcggcgtc atgtggccgg ctccccggct
       61 gccagtcgcc gcagcacctg ctcagagatc ccctttagcg tctcgaacac gcccccgcca
      121 tgcagggcat cggccgtgaa gctctgcacg gcgcggaagt tgagcgcgtc gtccagctcc
      181 gggggcgaca gaatcaaatc gcccggcaag tcctgcttgt tgtactgaaa gaccaccggc
      241 agctgacgga tgtcgatgtt ttgcgtgagc agattggtct ggagatcctg caggctttcc
      301 aaattgtcgt ccagccggcg ggcctgactg tcggcgacga aaacgacgcc gtcggcgccc
      361 tgcagcacca gcttgcgggt cgaggcatag tagctttgcc ccggcacggt gtagagctgg
      421 aatttggtcg cgaagcctga gatgctgccg aggtcgagcg gtagaaagtc gaagaacaac
      481 gtgcgatcgg tgtgagtcgc gagcgacacc atgcgaccac ggcgtgattc gggaacccgc
      541 ccgaagatgt actgcagatt ggtcgtcttg cccgaccgtc cggggccgta gtagacgatc
      601 ttgcaggtga tttcgcgggc ggcaaaattc acgagtgaca tcgcgtcacc gcccgaagag
      661 ggcgcgaagg ctcgcattga gctcccgctc gaagttttcc ggcaggactt gctgttccgg
      721 cgcctgccgt ggcgcagcag ccgcaaggtc ggaaatcagc cggtcgaaga acagctgcac
      781 caacccgacc gtactctccg cgtcgaacac caccaggccg atccagcggc cgcgaggtgt
      841 gtcgaacccc gccagataca cgtgctgcct ggcaccctgg tgcaccagcg agagaaaact
      901 cctctcgccc acgagcttgg cgatctctcc ggtagaagcc atgatcgcag ccccgagtgc
      961 ggccgcggtc atcacgtcca cggaccgggt gaagccgtgc tgcgcgatga cctgccccgc
     1021 cgaggtcata agcagggtga gccgagcgga cgactccgcg aggaagcgct cgagcggctc
     1081 tgtaacccag ggctcgacgc ttcgcatcag accagccttc cagcagcttc gagcatctcg
     1141 agacgcgcga gtcccacgtt cgcatccgga cccgccacgg cgaccagcac cagctccgca
     1201 ccgcatggag ttgccaggac cgagccactc tgcgcctgta gttgcaggaa gaccggctcc
     1261 ttgcggccgg cgcactcagt ggctcggcgc aaacgattca tgaggctcgc ggccagggca
     1321 gcgaccgctc gcccgtccac accggtcatc agggactcgg cgacgatgag tccgtcggca
     1381 gcggagacga gtaccgagcc gcgcagcccg cgaaccgtcg agaggcgctc cagcagttcg
     1441 acgggattac tcattcggct cgctccaacc atgaccgcgc ggccactgat gcccgctccg
     1501 cgaagcgcgc gacgcggccc attgggacgt cgagtccgcg cacggtgagc agcagagtcg
     1561 attcactggg cgcgctgaga tacagattcc cgtcgccgca ctcgacggag agcccacgcc
     1621 agtcgcccag ggtcaggagc cgcgccgtcc gggcggcttc cttggacacg cccgccaaca
     1681 gagccgcgac cgagtccgcg acatcgaccc cgtccgggtt gcgcaatcca ccacccagcc
     1741 ggaggccgtt ggcgtccacc aagagcagtc cgtcggcgcc gccgtcgagc ccgctgaaca
     1801 ccgcttcctg caccggcgag cgggcgcctt catcgaacga gaacgcgagt ccgcccgtcg
     1861 ccgcgatggt ggggagagaa gcatcgcccg gcgccgcagc atcggtgccg gggtcggact
     1921 gtgctgccgt gactgcttca ggagccgtgg cggcggcctg gggcgctcga ctcgcagccg
     1981 cgtccgcccg cacccgctcc agcgcagctg cagtcccgct gtcgtcggga tcgacttgag
     2041 tggaataggc caggtgcttc tcggccgagg cgaggtctcc ggcttgatag tagaggaagc
     2101 cgaggccctt gtgggcggac accagaccgg catcgagctg caacgccgtg acccaggact
     2161 cgaacgcgcg gtccggctcg ccccgatcgc agagaatgcg ggcgaacaga tcatgggcct
     2221 tcgccagctc cggataacgg ctgagcccgg tgcgagccac cttgaacgcg gcatcgagct
     2281 gcccccgccg gcgaagggct tcgccgaggt cgaggaaggt caggctgtcg ggatcggcgg
     2341 cgagctccgc cgtcatgccg cggatatcgt cagacatcgc gttcccccag cacgtgagcc
     2401 aaagtctcgg atgcctcgcg gagacgggcg acggcgttgg cgtgctcccg ctcgccgggt
     2461 tgattgcctg cccggctcca gcggtcgcag ctctcgatcg catcggcaaa tcgtccctgt
     2521 gcgacctgcg ccagcgccag gaggcggcgg ggcgcgccca gggtgggatc gcggtggagc
     2581 gcctgaatca gcgagtcctc tgcttcttcc caccgggcca ggcgaatcaa ggtgcgagcg
     2641 agcgcgacgt agccgtcggg ccgctccgga tccgcagcga ccagatcgac agcggccagg
//
LOCUS       DASRJQ010000114         4027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1100763, whole genome shotgun sequence.
ACCESSION   DASRJQ010000114 DASRJQ010000000
VERSION     DASRJQ010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100763"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..2891
                     /locus_tag="VFB89_02670"
     CDS             252..2891
                     /locus_tag="VFB89_02670"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM26235.1"
                     /translation="MARVPSMLTTDAERVAAELSDRYTIEEILGQGSSAVVFRALDRR
                     HQRPVALKVLSVAAGEKLGTGRFEREILTLARLQHPHILPLFDSGVAVGRLWFTMPFV
                     EAGSLRDRLKARGPLSQAEATQLATELGEALAYAHAHGVIHRDIKPENIMFSAEGHAL
                     LADFGLARATDQELSPSVTDAGVAVGTPLYMSPEEAGGEGADERSDVYGLGSVVYEAL
                     TGEPPFTGTNARAVMMRRLVEPPPSARSLRPELSKAMDAALLKALARHPEQRFSTPAD
                     FASALAGTTAVRSQDRLRWWAIAAFVLVAVAAAGALLWRSPRTVPVAEGSSIPVLAVL
                     PFKNLGPPDEQYFADGLTEEITSRLAGLSGLRVISRTSADQYRNATKPLRDIGQELGA
                     AYVLEGSVRWERTPGTPGRVRVTPQLIRVADDSHLWADAYDAELTRIFALQASIAEQV
                     TRALDLALRPPERTALVEGGTADAEAYDYYLQGNDYLGRGNARATLATAAELYGKAVS
                     RDPRFALAFARLSNARSQMYWYHHEARTPALIALAKRAADSALALVPDLAEGQTALGY
                     YHYRGFRDYAQALVHFERARRRQPHNTELLAGIGYVERRRGRWNEAIEAFAEAARSDP
                     RSNVRAFDLGTSLNAVRRFAEAERELDRAITLAPDWASPYGEKAQLVLIARGDAAGAR
                     AVIRQALTRVGLGPLSRSVFSTDQIVGPLFSSDSAIGPLVDSLRLQGFAGDTLHYYFL
                     KTESALFRQRAAGQRAYADSLRRWLERELDPNDAYDLNRLAFAYAALGRKAEAVRAAR
                     RSVELMPVSRDALIGPYFVVGLARTYMMVGEPDRAAETLAPLLEIPSPITRAGLAADP
                     LWAPLRENPGFQKLIAEVRQPAE"
     gene            complement(2872..3630)
                     /locus_tag="VFB89_02675"
     CDS             complement(2872..3630)
                     /locus_tag="VFB89_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HZM26236.1"
                     /translation="MSIDHYVALGDSMSSDHYPTCDLRNLDAPPSHLDPAGAAALLFR
                     NDDARWPEFSGQDLARMAAGVDFRNLTEDGALIDDVRTEELSRLGRDSADPRVLVTLT
                     AGGNDLLDALLAGVPLDRAVGRIAGRYTDLIDALLEELPRATLVLTTVYDPTDGTGIL
                     PGLFEVKGRLPLEYLDQFNQHVRDTAGQTPGALLADVHQHFLGHGVSLSESGRWYWRH
                     NLIEPNARGASEIRRVWWEVLQQSTQRIPTLPAA"
     gene            3724..>4027
                     /locus_tag="VFB89_02680"
     CDS             3724..>4027
                     /locus_tag="VFB89_02680"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HZM26237.1"
                     /translation="MQVFGTHDDATRAQLARVAEHARKVALMADGHVGYVMPIGGVAA
                     YDNQVSVVGVGFDIACGNAAIRTDRKLGDIGGAPEVAARRLGQLADQINDTVSFGIG"
BASE COUNT          647 a         1341 c         1380 g          659 t
ORIGIN      
        1 cttgagcgcg acgaggcgat cgtgcttgag gtcccgcgcg agatacacgg tagccattcc
       61 gccggcgccg agctgacgct ccagcgtgta gcggttgcgt agagccccgc ggagcgacgc
      121 cgcaacgtcg gtcaggagga cctatcccag gctgaggagt gtcctaatct gtgcgggttc
      181 acctgtaacg gccagcagaa ttcgcagcgg atgcaaacag gggaggcggc aggttcctac
      241 ctccgctaat tttggctcgg gttccctcca tgctcacgac cgacgccgaa cgggttgcgg
      301 ccgagctcag cgaccgctac accatcgaag agatccttgg ccagggctcg tccgccgtgg
      361 tctttcgggc gctcgatcgg cgccaccagc gtcccgtcgc gctcaaggtc ctttcggtcg
      421 cggccgggga gaagctgggc accgggcgct tcgagcgtga gatcctgacc ctcgcgcgtc
      481 tgcagcatcc ccacatcctg ccattgttcg actctggcgt cgccgtcggg cggctctggt
      541 tcaccatgcc gttcgtcgaa gcggggtcgc tgcgcgaccg attgaaagcg cgcggcccct
      601 tgagccaggc tgaggcgacg cagctcgcga ccgagttggg ggaggcgctg gcgtacgctc
      661 atgcccacgg cgtgatccac cgcgacatca agcccgagaa catcatgttc tccgccgagg
      721 ggcacgcgtt gctggccgac ttcggtctgg cgcgcgcgac tgaccaggag ctctcgccgt
      781 cggtgaccga tgcgggcgtt gcggtgggga cgccgctcta catgagtccc gaagaagcgg
      841 gtggcgaagg tgccgacgaa cgcagcgacg tgtacggcct gggctccgtc gtctacgagg
      901 cactgaccgg ggagccgccg ttcaccggca ccaacgcccg cgccgtcatg atgcgacggt
      961 tggtggagcc tcctccttca gcacgcagtc tgcgccccga gctgtccaag gcgatggatg
     1021 ccgcactgct caaggcgctg gcccgtcatc cggagcagcg gttctccacg cccgccgact
     1081 tcgcctccgc actggcgggg acaaccgctg tgcgctcaca ggatcgcctt cggtggtggg
     1141 ccattgccgc cttcgtgctc gtggcggtgg ctgcggccgg tgcgctgctc tggcgctcgc
     1201 ctcgaaccgt gcccgtcgca gaaggctcga gcattccggt gcttgccgtg ctgcccttca
     1261 agaatctcgg cccgcccgac gagcagtact tcgccgacgg gctcaccgag gagatcacca
     1321 gccggctcgc gggcctgtcc ggactccgtg tcatctcgcg caccagcgcc gaccagtatc
     1381 gcaacgccac gaagccgctg cgagacatcg gccaggagct gggagcggcc tacgtgctcg
     1441 aggggagcgt gcggtgggag cgcacgccgg gaacaccggg ccgggtgcgc gtcacacctc
     1501 agctgatccg cgtggccgat gacagtcatc tctgggccga cgcgtacgac gccgagctca
     1561 cccggatctt tgcgctgcag gcaagcatcg ccgagcaggt gacccgggct ctggacctgg
     1621 cgcttcgtcc gccggaacgc acggcgctgg tggagggtgg taccgctgat gccgaggcgt
     1681 acgactacta cctccagggc aacgactacc tgggccgggg caacgcaagg gcgacactcg
     1741 cgacggcggc ggaactctat ggaaaggcgg tgagccgtga tccgcggttc gccctcgcct
     1801 ttgcacggct ttcgaacgcg cgcagccaga tgtactggta ccatcacgag gctcggacgc
     1861 ctgcgctgat cgccctggca aaacgggcgg ccgattcggc gcttgccctc gtgcccgatc
     1921 tggcggaggg gcagacggca ttgggctact accactaccg tggcttccgg gactacgccc
     1981 aggccctagt gcacttcgaa cgggcgcgcc gccggcaacc ccacaataca gagctgctcg
     2041 cggggatagg gtatgtcgag cgacgccgcg gccgctggaa cgaggccatc gaggcgttcg
     2101 ccgaggccgc ccgatccgac ccgcggtcca acgtccgggc gttcgatctc ggcacctcgc
     2161 tgaacgctgt ccgtcggttc gccgaggcgg agcgtgagct cgaccgagca atcaccttgg
     2221 cgcccgactg ggcgagtccc tacggggaga aggcccagct ggtgctcatc gcgcgcggcg
     2281 acgctgctgg agcgcgcgcc gtgattcggc aagccctgac cagggtcggc ctgggcccgc
     2341 tgagtcgctc cgtgttttcc accgaccaga tcgtggggcc gctcttcagc tcggacagcg
     2401 caatcgggcc gctggtcgac tcacttcgcc tccagggatt cgccggcgac acgttgcact
     2461 actacttcct caagacagag agcgcactgt ttcgccagcg cgcggcgggc cagcgagctt
     2521 atgccgattc gctccgcagg tggctggagc gagagctcga tcccaacgac gcctacgacc
     2581 tcaaccggct cgccttcgcc tatgccgcgc ttggccgcaa ggccgaggcg gtccgtgcgg
     2641 ctcggcgctc ggtcgagttg atgccggtct cccgggatgc gctgatcggc ccgtacttcg
     2701 tggttgggct tgcacgaacc tacatgatgg tgggggagcc cgatcgagcg gccgagacgc
     2761 tggcgcccct gctcgagatc ccgtcgccga ttacgcgggc ggggctggcg gcggacccgc
     2821 tgtgggcccc gctcagggaa aatccaggat tccagaagct gattgccgag gtcaggcagc
     2881 cggcagagta gggatccgtt gggtcgattg ctgaagcacc tcccaccaca cgcggcggat
     2941 ctcgctcgcc ccgcgcgcat tcggctcgat caggttgtgt cgccagtacc agcggccgga
     3001 ttcgctcagg ctgaccccat gcccgaggaa atgctgatga acgtcggcaa ggagagcacc
     3061 cggcgtttgg ccggcagtgt cacggacgtg ctggttgaat tggtcgaggt actcgagcgg
     3121 aagccggcct ttcacttcga acagccctgg gaggataccg gtgccgtcgg tcgggtcgta
     3181 caccgtcgta aggacgagag ttgctcgcgg cagctcctca agtaaggcgt cgatcagatc
     3241 ggtgtagcga cctgcgatcc gtcccacagc acgatcgagc ggcacccccg ccagcaacgc
     3301 gtcgagcagg tcgttcccgc ccgccgtcag cgttaccaat acccgcgggt cagcggaatc
     3361 ccgccccagc cgggacaact cctcggttcg cacgtcatcg atcagggcgc cgtcctccgt
     3421 aaggttgcgg aaatccacgc cggcagccat gcgcgcgagg tcctggccgc tgaactcagg
     3481 ccaacgcgcg tcgtcgttgc ggaagagaag ggcggcggcg ccagccggat ccaggtgact
     3541 cggcggcgca tcgaggttcc gaaggtcgca ggtgggatag tggtcgctgc tcatggagtc
     3601 gcccagcgcc acgtagtggt cgatcgacat ggaccaaatg aacccggctg gtccagcccg
     3661 cgccacccat gaagccttgg cggttccggt ccgccgctct tacatctcac ttcgggaggc
     3721 gccatgcagg tattcggaac ccacgacgac gcaacgcggg cgcagctcgc gcgagtggcg
     3781 gagcatgcgc gcaaggttgc cctgatggcg gacggtcacg tgggctacgt catgccgatc
     3841 ggcggcgtgg cggcgtacga caatcaggtg tcggtggtgg gagtgggatt cgacatcgcc
     3901 tgcgggaacg cggcgatccg gaccgatcgg aagctgggcg acatcggcgg cgctccggaa
     3961 gttgcggcca ggcggttggg ccagctggca gaccagatca acgacaccgt gagcttcggg
     4021 attggcc
//
LOCUS       DASRJQ010000115         4363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3866313, whole genome shotgun sequence.
ACCESSION   DASRJQ010000115 DASRJQ010000000
VERSION     DASRJQ010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3866313"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VFB89_02685"
     CDS             <1..478
                     /locus_tag="VFB89_02685"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HZM26238.1"
                     /translation="EEAGATWAARSTDEEEAEALFAARRLAYPALERLGPVLTEDVCV
                     PIAAVPDMLDRIEHIASTHDILIASIAHIGDGNLHPLLIADPGDAAARDRAKIAFEEI
                     LSAALDLGGTVTGEHGVGLLKRGGLSRELAPAVLEMHRAVKAALDPHHILNPGKIF"
     gene            516..1964
                     /locus_tag="VFB89_02690"
     CDS             516..1964
                     /locus_tag="VFB89_02690"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH and CHAD domain-containing protein"
                     /protein_id="HZM26239.1"
                     /translation="MRKITEQEDKSEVDTDWVMPQLTELLPDGGRLDQEIRTLDSTYF
                     DTPGAGLRLFGITLRRRVGGSETGWQLKVPSGTARTELQSGSWTETLPAALENGVDGL
                     LAGENLGAVATIKTTRTAYQLLNADGDLLMEIADDQVESGPPDGESMLHSWREIEVEL
                     GPAGKKKDLKQARKLLQAAGAAPSTIRTKLDKALGPALPDRQAFTIEAGSVGELVAAY
                     VAAQCEVLASNDVGMRTGEPAVHKTRVAARRLRSTLRMFDDVFVAAPAEELDNELSWY
                     ADVLGQVRDRDILSSRLTKQIAELPPVHLRGPVEAEITKALAAEREEAVEELNEAIRT
                     PRYQHLVQLLRGWRAAPPVTDAAGKKAKTAVKYVDKAKKKADKRLRKADEDIDRLHRA
                     RKAMKRARYAAELVEPANSDMKAVAREAEEIQTLLGEHQDAVVSAEFLAKISSSADGE
                     SEESTFTYGVLMANELQRAADIRASLQSSGSI"
     gene            2004..2396
                     /locus_tag="VFB89_02695"
     CDS             2004..2396
                     /locus_tag="VFB89_02695"
                     /inference="COORDINATES: protein motif:HMM:NF022359.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2695 domain-containing protein"
                     /protein_id="HZM26240.1"
                     /translation="MSAESDLAPVLHIERELRHLSVLITQPKPAECLHCYVYRMLEFG
                     CSGLRWARRYRDLKAPRATALDRRLMSKGAGCDCEIFMNGWSLRREYRLYDPESEEYE
                     YPDELPACRGVRVGSIQPCGLWTTYSRW"
     gene            complement(2409..3278)
                     /locus_tag="VFB89_02700"
     CDS             complement(2409..3278)
                     /locus_tag="VFB89_02700"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZM26241.1"
                     /translation="MRVALVGAGRMGSAMGIRIAEAGHDLTVFNRTRSRCEDVAVRTG
                     AQVADTCREAAEFAEVCVVSLADDTAVTSTYLDDEGLIAGVQSGAVVCDMSTVAPATV
                     RSLAPLVAQKDATLLDTPVSGSVSVVESGTLTVMVGGDQDALDRARPVLESFAKSIFH
                     LGDVGAGATMKLVVNSLVHSLNVAVSEALVLAEKAGLDRETVYDIFEAGAAGAPYVKY
                     KREAFLKPGEVPVAFSLGLVAKDQDLIHDLAELTATRMDQAESSRQLVAEALSAGMAE
                     RDISEVAEFLRRS"
     gene            complement(3275..3967)
                     /locus_tag="VFB89_02705"
     CDS             complement(3275..3967)
                     /locus_tag="VFB89_02705"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZM26242.1"
                     /translation="MTALLLVGGTRGIGKEIARYYAKQGWSIVLTGRHASHAQSAAAE
                     VDGETRGIALDLSEPEQVAGALSDVGAVDHVVLTSIARDTNYCEDYDIPSARYLAIMK
                     LVGYTAVISALTDRLSDDGSIVLFGGRAKDRPYPGSLTVSTVNGGISGMMRALAVQLA
                     PRRINAIHPGIVGDSPYWTGNQEFTETVRQATPTGRNVTIADIVDAVDFLMRNRSVNG
                     IDLYVDGGWLLQ"
     gene            complement(3964..>4363)
                     /locus_tag="VFB89_02710"
     CDS             complement(3964..>4363)
                     /locus_tag="VFB89_02710"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZM26243.1"
                     /translation="RSARGRRPAGADTALLVYSGIGVKMLIDKVFGADHLTMFTVDYD
                     VGGAAQKHDHPFEETYFFLEGQAEATFDGKPVVLGAGDIAWAGVGCPHSFRNLGGGPL
                     RWLETQAPQPPPRYSYRFARDWDYLREVLR"
BASE COUNT          877 a         1341 c         1378 g          767 t
ORIGIN      
        1 tgaggaggca ggcgccacct gggcggcccg ctcgaccgac gaggaggagg ccgaagcgct
       61 cttcgccgcc cgccggctcg cgtacccggc gctggagcgg ctaggtccgg tcctgaccga
      121 agacgtgtgt gtaccgatcg cagccgtgcc ggacatgctg gaccgaatcg agcacatcgc
      181 cagtacgcat gacatcttga tcgcaagcat cgcccacatc ggggacggca acctgcatcc
      241 attactcatc gccgacccgg gcgacgcggc cgcgagggat cgcgccaaga tcgccttcga
      301 agagatactc agtgccgcac ttgatcttgg aggcactgtg acaggcgaac acggcgtcgg
      361 gttgctcaag cgcggcggac tctcccgtga gctcgctcct gcggtactgg agatgcatcg
      421 cgcggtcaag gcggccctgg atccgcatca catcctcaac cctggcaaga tcttttgaat
      481 caatggcaaa gttgcatgga aggagggccg cagaggtgcg aaaaattaca gaacaagaag
      541 acaagtccga ggtcgatacc gattgggtga tgccccagct caccgagttg ttgccggacg
      601 gcggccgact tgatcaagag atccggacgc tcgatagcac ctacttcgac acgccaggcg
      661 ctggtctgcg gttattcggg attacgctga ggcgtcgcgt gggaggctcc gagaccggct
      721 ggcagctgaa ggttcctagc gggacggctc gcacggaact gcaaagcggt tcatggacgg
      781 agacgcttcc agctgcattg gaaaacggcg tcgacggtct attggctggt gaaaacctgg
      841 gtgccgtcgc aacgatcaag acgactcgaa cggcgtacca gttgctcaat gccgacggtg
      901 atctcctgat ggagattgcc gatgatcagg tggagtccgg tccgcccgac ggcgagtcga
      961 tgctccactc gtggcgtgag atcgaggtgg agctgggacc agccggcaag aagaaggacc
     1021 tgaagcaggc acgcaagctg ctgcaggctg ctggcgccgc ccccagcacc atcaggacaa
     1081 agctcgataa ggcgctgggg ccggcattgc cggatcggca ggcattcacg atcgaagccg
     1141 gctcggtcgg agagctggta gccgcgtacg tcgcggcgca gtgcgaggta ctcgccagca
     1201 atgacgtcgg aatgcgcacc ggagagcccg ctgtacacaa aacacgggta gcagcgcgcc
     1261 ggcttcggag cactctgcgg atgttcgacg acgtgttcgt cgctgctcct gctgaggagc
     1321 tggacaatga actgtcgtgg tatgccgatg tgcttggcca ggtgcgtgac cgggacattc
     1381 tgagcagccg actgaccaag cagatcgccg aattgccacc agttcacctt cgcggaccgg
     1441 tcgaagccga gatcaccaag gcactcgctg cggaacggga ggaggccgtc gaagagctga
     1501 acgaggcaat ccgcaccccg cgttatcaac atttggtcca gctgcttcgc ggttggcgtg
     1561 cggcaccacc ggttaccgat gccgcgggca agaaggccaa gactgcggtc aagtacgtgg
     1621 ataaggccaa gaagaaggcc gacaaacggt tacgaaaggc cgacgaggac atcgatcggc
     1681 tgcatcgagc acgaaaggcg atgaagcggg cgcgctacgc cgctgagctt gtcgaaccag
     1741 ccaacagcga catgaaagct gtagcacgtg aagcagagga gatacagacc ctgctcggtg
     1801 agcatcagga tgccgttgtt tctgccgagt tcctggccaa gatcagttcg tcggccgacg
     1861 gcgagagcga ggagagcacc tttacctacg gggttctcat ggcgaacgag ttgcaacgtg
     1921 cagcggacat tcgcgcctcc ctgcaaagta gcggctctat ttgacgctct cgtctgtcag
     1981 tgccgggcga tagggatgta ggtatgagtg ctgaatccga cctggctccc gtgctgcata
     2041 tcgaacgaga gcttagacat ctatcggttt tgatcaccca gcccaagcca gccgaatgcc
     2101 tgcactgcta cgtgtatcgg atgctggagt tcggctgctc cgggcttcgc tgggccaggc
     2161 gctatcgcga cctcaaggcg ccccgagcga ccgccctcga cagacgcctg atgtctaaag
     2221 gagctgggtg cgattgcgag atcttcatga acggttggag cctgcgtcgc gaataccggc
     2281 tgtacgaccc agagagtgag gagtatgagt atcccgacga gttgccggcg tgtagaggtg
     2341 ttcgggtcgg gtcgatccag ccctgtggtc tctggaccac ttactcccgt tggtgaacaa
     2401 gtgcccagtc aggaacgccg tagaaactcc gcgacctcgc taatgtcgcg ctcggccatc
     2461 ccggcgctga gagcttctgc taccaactgc cgcgatgact cggcctgatc catccgcgtt
     2521 gctgtcaatt cggcaagatc gtggatgagg tcctgatcct tggcgaccag ccccaggctg
     2581 aatgcgactg gaacctcacc gggcttgagg aaggcttcgc gtttgtactt cacgtaaggc
     2641 gcgccggcgg cgcccgcctc aaagatgtcg tacaccgtct cgcgatcaag tccagctttt
     2701 tcggcaagca ccagcgcctc gctgacggcg acgttgagcg aatgcacaag agagttcacg
     2761 acgagcttca tcgtggcgcc agctccgacg tcgccaaggt ggaagatcga ctttgcgaag
     2821 gactcaagca ccggacgcgc ccgatcaagc gcatcttgat ctcccccgac cattacggtt
     2881 agcgtccccg actcgacgac cgagacactt cccgagaccg gggtatcgag cagggttgca
     2941 tccttctgcg cgaccagcgg tgccaagctg cggactgtcg caggcgcaac tgtgctcatg
     3001 tcgcacacaa ccgcacctga ctgaacgccg gcgatcaagc cctcgtcgtc gaggtaagtc
     3061 gaggtcacgg ccgtgtcgtc ggcaagggag acgacacaga cctcggcgaa ctccgccgct
     3121 tcgcggcagg tgtctgctac ctgcgcgccc gtgcgcacag cgacatcttc acaacgcgat
     3181 cgcgtgcggt tgaatacggt gaggtcgtgg cccgcctcag cgatgcgaat acccatcgcg
     3241 cttcccatgc ggccggcgcc gaccagtgcg actctcattg cagcagccag ccgccgtcga
     3301 cgtacaggtc gatgccgttg accgaccgat tgcgcatcag gaagtcgacc gcgtcgacga
     3361 tgtcggcaat cgtgacgttc cgcccggtcg gcgtcgcctg ccggaccgtc tcagtgaact
     3421 cctggttccc cgtccagtag gggctgtcgc cgacaatgcc cggatggatc gcgttgatcc
     3481 ggcgcggagc aagttgcaca gccaacgccc gcatcatgcc agagatgccg ccgttcacgg
     3541 tggagacagt caatgaacct gggtatggac ggtccttggc ccggccgccg aacaacacga
     3601 tcgagccgtc gtccgacaac cggtcggtca acgcactaat aaccgctgtg tagccgacca
     3661 gtttcatgat cgcgagatag cgcgccgagg ggatgtcgta gtcctcacaa taattcgtgt
     3721 cgcgggcgat cgacgtcagt actacatggt cgacggcgcc tacatcgctc agcgccccgg
     3781 caacctgctc cggctcggag aggtccagag caatgccccg tgtctcgccg tctacctccg
     3841 cagccgccga ctgggcatgc gaagcgtgac gtccggtgag cacaatcgac caaccttgct
     3901 tggcgtaata gcgcgcgatc tccttgccga taccgcgggt gccacccacg agcagcagcg
     3961 ccgtcatcga agtacctccc ttaggtagtc ccagtcgcga gcgaaccgat aggaataacg
     4021 cggcggcggc tgtggagctt gggtctccag ccaccggagt ggcccgcctc cgaggtttcg
     4081 gaaggagtgc gggcacccca cccctgccca ggcaatgtcg ccagcgccga gtacgacggg
     4141 cttgccgtcg aaggtcgcct cagcctgtcc ttcaaggaag aagtacgtct cctcgaacgg
     4201 atgatcgtgc ttctgggccg caccgccaac gtcatagtcc accgtgaaca ttgtcagatg
     4261 gtctgcgccg aagaccttgt cgatgagcat cttgaccccg atgccgctgt acacgagcag
     4321 ggcggtgtcg gcaccggctg gtctgcggcc gcgggcagat cgg
//
LOCUS       DASRJQ010000116         5262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_766201, whole genome shotgun sequence.
ACCESSION   DASRJQ010000116 DASRJQ010000000
VERSION     DASRJQ010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5262
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766201"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="VFB89_02715"
     CDS             <1..1052
                     /locus_tag="VFB89_02715"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HZM26244.1"
                     /translation="ESPTPASLGFSMPPEWAPHRGTWLSWPHKEASWPGKFEPVPAIF
                     ARMVSVLAEQEEVHINVADEEMEAGTRSLLTRTGASMNRVFFHRNPTDDAWCRDHGPI
                     FLQRQSNGKAEQAIVDWDYNAWGGKYPPFAQDDVIPTRIGEELGLQVFHPGIVMEGGS
                     LDVNGKGTLLTTEACLLNPNRNPRLARSEIEDYLRGYLGVSNVLWLGDGIAGDDTDGH
                     VDDLTRFVDPTTVVTVVEEDPRDENFEPLQENLQRLRAMKDQDGRPLRVETLPMPRPL
                     YHEGQRLPASYANFYIANGVLLLPGYDPDQDRKAQATLERLFPDRRIVVIDCTDLVWG
                     LGAFHCVTQQWPRTK"
     gene            complement(1056..1457)
                     /locus_tag="VFB89_02720"
     CDS             complement(1056..1457)
                     /locus_tag="VFB89_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26245.1"
                     /translation="MPVWFRGATVAPSLALLGLLTVTACGGEGGKYAGNYKRDLYGEG
                     EVQMKLSGDGTVELVLPSPRWAANPNRKGKGTFKGDTLVFPVDTAAVKCATQEARYVL
                     AESDGNLVVAGVGMDNCGDRRAALNGAWKKG"
     gene            complement(1610..2902)
                     /locus_tag="VFB89_02725"
     CDS             complement(1610..2902)
                     /locus_tag="VFB89_02725"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF006681.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HZM26246.1"
                     /translation="MKHVLEAELTWRGDRFEPHIQVAVGDDGRIETVGQLGREPTRRM
                     PQQALLPGFVNAHSHAFQRGLRGSGERFPAGAGSFWTWRNAMYELADSLDPETAHALY
                     LRAFGEMRDAGITTCGEFHYLHHLGSTRDYEFDEVVLDAARQAGIRLALLNAFYTTGG
                     VGRALRPAQRRFESKDLPSYWAQMDRLQACAEPQLQTVGVVAHSVRAAALEAIIALHA
                     EAQRRGLRFHMHVEEQRGEIEECLAGYGRRPMELLNEALTIGGNLTAVHCTHTDPRDM
                     TRFLAAGGSVCVCPLTEGNLGDGLPALDLVHAAGGRLSLGTDSNARISMLEEMRWLEY
                     GQRLRREQRGVLTDSGGRVATALLDAATAGGAAALGVRTGRLQAGCWADFVAIDLEAP
                     SLAGLEPENLLEGVIFGAGDKVIAGTMIAGGWRPTGGS"
     gene            complement(2899..4062)
                     /gene="argE"
                     /locus_tag="VFB89_02730"
     CDS             complement(2899..4062)
                     /gene="argE"
                     /locus_tag="VFB89_02730"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HZM26247.1"
                     /translation="MARLMTPEALLARLVAFDSSSQVSNLPIADFICDYLDAPDVRIF
                     RNLSANGEKVNLVIARGPRDEGAGGLTLSGHLDTVPFAEPDWTSDPLALTRQDDRLIG
                     RGACDMKGFLALAIHRFASLNRSALRHPLALLLTCDEELGTLGSRRFVETWSGDEPLP
                     RAVVIGEPTSLSVIRLHKGHLKLRIIIQGRAGHSAYPASGVNAIELAGGVIDRLRQLS
                     RSLAAEMSREGALFGPVPHATLNLATISGGVALNVIPEHCVLELGIRTMPGMTSSDVT
                     GRVRRAVAAAVGGCGAVELIGDSPPLRTSELAPIHVALARLMNQGDSRAVAFASDGGW
                     LQTMGLDCVLWGPGSIEAAHRADEWLPVSEFERAAGLLERLVHQSCVAESSKP"
     gene            complement(4047..>5262)
                     /gene="hutI"
                     /locus_tag="VFB89_02735"
     CDS             complement(4047..>5262)
                     /gene="hutI"
                     /locus_tag="VFB89_02735"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HZM26248.1"
                     /translation="LRNIGLLATCRAEGLQQDIHPIPRAALAWDDGRVVWAGPESDLP
                     RHLEAGETLDAGGRLVIPGLVDCHTHLAFGGWRADDFERRIRGESYLEIAASGGGILS
                     TVRATRACSEEALLKRGAGVLDDMVALGVTTVECKSGYGLDEASELKILRVYQKLAEL
                     GPMRLVPTLLAAHTVAPEYRDRRSAYVDLVVDRIIPAAGRERLARCCDVFVEDSAFTV
                     EEARRILTAGRAAGLAPKLHADQITAGGGAELAGELGALSADHLENVSRGGIDAMQRA
                     GVVAVSLPIATLYLGQAPMPARRLIEAGVRVAVATDFNPGSAPSWHLPLALTLACTLQ
                     RMTPAEAVKGATIHAARAIGLEAEIGSLEPGKAADFAIIDAPDVNHWLYQFRPNACLQ
                     TVIRGATRWRAS"
BASE COUNT          960 a         1790 c         1642 g          870 t
ORIGIN      
        1 tcgaatctcc gactccggct tctctcggct tctccatgcc gcccgaatgg gcgccgcatc
       61 ggggcacctg gcttagttgg ccccacaagg aggcgtcgtg gcccggaaag ttcgagccgg
      121 tgcccgccat cttcgcgcgc atggtcagtg tgttggccga gcaggaagag gtgcatatca
      181 acgtcgctga tgaggagatg gaggccggca cgcgcagcct gctcacgcgg accggagcgt
      241 ccatgaatcg cgtgttcttt caccggaatc caaccgacga tgcctggtgt cgcgatcacg
      301 ggcccatctt cctgcaacga cagtcgaatg ggaaggctga acaagcgatc gtcgactggg
      361 actacaatgc ctggggtggg aaatacccgc catttgcgca ggacgacgtc atcccgactc
      421 ggatcggcga ggagcttggc ttacaggtct tccaccccgg gattgtcatg gaaggcggct
      481 cactcgacgt caacggcaag gggactctgc tcacgaccga agcctgcctc ctcaatccca
      541 accggaaccc ccgcctggca cggagtgaga tcgaggacta cctcaggggg tacctcggcg
      601 taagcaacgt tttgtggctg ggcgacggca tcgccggcga tgacaccgat ggacacgtgg
      661 acgatcttac ccgcttcgtg gacccgacta cggtcgtcac cgtcgttgag gaagaccccc
      721 gggatgaaaa cttcgagccg ctgcaggaaa acctgcagcg gctccgcgcc atgaaggacc
      781 aggacggtcg cccactccgg gtggaaaccc ttcctatgcc ccggccgctc taccatgagg
      841 gccaacgact cccggccagc tacgccaact tctatatcgc gaacggcgtc cttctactgc
      901 cgggctacga ccccgatcaa gatcggaagg cccaggcgac tctggagcgg ctgtttcccg
      961 accggcggat tgtcgtcatc gactgtaccg atctcgtctg gggcctgggt gccttccact
     1021 gcgttaccca gcaatggccc cgtacgaagt aagcctcagc ctttcttcca ggcgccgttc
     1081 agagccgccc gccggtctcc gcagttgtcc atcccgacac ccgcgacgac cagattccca
     1141 tcgctctccg ccagcacata acgggcttcc tgggtagcac acttaaccgc cgcggtatca
     1201 actgggaaca ccagcgtgtc gcctttgaaa gttcccttcc ctttcctgtt gggattcgcg
     1261 gcccagcgcg gcgaaggaag aacgagctcc acggtgccat caccgctgag cttcatctgg
     1321 acctcgccct caccgtacag gtcgcgcttg taattgccgg cgtatttccc tccttcgccg
     1381 ccgcaggcgg ttaccgtgag gagtcccagc aaagccagcg atggcgcaac agtggcacca
     1441 cgaaaccaaa ccggcatcag tctaccctcc tctgtggcct acgagatttc tgtggcacgg
     1501 cttcgccgac gacatggcaa gcctcacata atgtggcagc ctgacgggac gcgccagtag
     1561 cgaggccgtc gaccggcctc ctctccgagg caatggctcg agcgggaggt cacgaacccc
     1621 cagttggacg ccagccgcca gcgatcatgg tccccgcgat caccttatca ccggctccga
     1681 aaatgacgcc ctcgaggagg ttctcaggct cgagcccggc aagggaaggc gcctcgagat
     1741 cgatcgccac gaaatcggcc caacagcccg cctgcagccg accggtccga acgcccaggg
     1801 cggcagctcc tccggcggtc gcggcatcca gcagggcggt cgcgacgcgg ccgcccgagt
     1861 cggtcagcac gcctcgctgc tcccggcgaa gccgctggcc atattccagc caccgcatct
     1921 cctcgagcat cgaaatccgt gcattagaat cggtgccgag actgagacgg ccgccggcgg
     1981 catgcaccag atccagagcc gggagcccgt ctcccagatt tccctcggtc aaggggcata
     2041 cgcagacgga gcctccggcg gcaaggaatc gcgtcatgtc gcggggatcg gtatgggtgc
     2101 agtgcacggc ggtcagattg ccgccgatcg tcaacgcctc gttgagaagt tccatcggcc
     2161 gccgaccgta tcccgccaga cactcctcga tctcaccgcg ctgctcttct acgtgcatgt
     2221 gaaacctgag gcctcgccgc tgggcctccg cgtgaagtgc gataatggcc tcgagcgccg
     2281 cggctcgtac gctgtgggct acgacgccca cggtctggag ctgtggctcg gcgcaagcct
     2341 gcagccggtc catctgtgcc cagtaggacg gcaggtcttt cgactcgaag cgtcgctgcg
     2401 ccggccggag ggcccgtccg acaccgcctg tcgtatagaa cgcgttgagg agcgccagcc
     2461 gaatgccggc ctgacgcgcg gcatcgagca cgacttcatc gaactcgtag tcccgggtcg
     2521 acccgagatg gtgcagatag tgaaattcgc cgcaggtggt gatccccgcg tctcgcatct
     2581 cgccgaatgc ccgcagatag agtgcgtggg ccgtctccgg gtcgagcgag tccgccagct
     2641 cgtacattgc gttccgccag gtccagaagc tgcccgcgcc agcggggaag cgctcgcccg
     2701 acccgcggag gccgcgttga aacgcgtggg agtgagcgtt gacgaagccg ggcagcaggg
     2761 cttgctgcgg catcctgcga gtcggctccc gtcccagctg tccaaccgtc tcgattcggc
     2821 cgtcgtcgcc gacagcgacc tggatgtggg gctcgaaccg gtcccccctc caggtcagct
     2881 cagcttcgag cacatgcttc atggcttgct cgactccgca acgcacgact ggtgcaccag
     2941 ccgctcgagc aggccggctg cacgctcgaa ctcggacaca gggagccact cgtccgctcg
     3001 gtgcgccgcc tcgatggacc ccggccccca gagcacgcag tcgagcccca tcgtctggag
     3061 ccagccgccg tcgctggcga acgccaccgc ccgggaatcc ccctggttca tgagtcgagc
     3121 aagagcgaca tgtatcggcg ccagctcgga cgttcgtaga ggcgggctat cccctattaa
     3181 ctcgacggcg ccgcagccac cgaccgccgc tgccaccgct cgacgtaccc gacccgtcac
     3241 atcagatgac gtcatcccgg gcatcgtgcg aatgccgagc tcgagcacgc agtgctccgg
     3301 aatgacattg agggccacac cgcccgaaat tgtggccagg ttcaaggtcg catgaggaac
     3361 cgggccgaag agcgcaccct ctcggctcat ctcggcggcc agcgagcggg agagctggcg
     3421 aagccggtcg atcaccccac cggccagctc gatcgcattg acgccagagg cgggatacgc
     3481 actgtgcccg gcccgcccct gaatgatgat gcgcagcttc agatgaccct tgtgcaaccg
     3541 gatgacggat agcgaggtcg gttctccaat caccacggca cgcggcaggg gctcgtcgcc
     3601 ggaccaggtc tcgacaaagc ggcgggagcc caacgttccc agctcttcat cgcaggtgag
     3661 aaggagcgcc agcgggtggc ggagcgcgct ccggttcagg cttgcaaagc gatgaattgc
     3721 aagagcgaga aatcccttca tgtcgcatgc accgcggccg atcagacgat catcctgccg
     3781 cgtcagcgcc agcggatcgc tggtccagtc gggctcggcg aatggaacgg tatccaggtg
     3841 gccggacagt gtgagtccgc cggcaccctc gtcgcgcggg ccgcgagcga tgacaaggtt
     3901 caccttctcg ccgttggccg acaggttacg gaagatccga acatccggag catcgaggta
     3961 gtcacagatg aaatcggcga tcgggagatt gctgacctgg ctggacgaat cgaatgcgac
     4021 caggcgcgcc aacagcgctt ccggggtcat gaggcgcgcc atcgggtagc ccctctgatc
     4081 acggtctgga ggcaggcgtt cggtcggaac tgatagagcc agtggtttac atcaggcgcg
     4141 tcgatgatag cgaagtccgc cgccttccca ggctcgagcg acccgatctc ggcctcgagg
     4201 cctatcgccc gggctgcgtg gatcgtggct cccttcactg cctcggctgg agtcatgcgc
     4261 tggagcgtgc aggcgagggt gagagccaac ggtagatgcc agctcggcgc cgacccggga
     4321 ttgaagtcgg tcgcgaccgc cacccgcaca ccggcctcga tcaggcggcg tgccggcatg
     4381 ggggcttgtc cgagatagag cgttgcaatc ggcaggctca cggccaccac gcccgctcgc
     4441 tgcatggcgt caatcccgcc tcgggagacg ttctccaggt gatccgcgga cagggcacca
     4501 agctctcctg ccaactcagc gccgccacca gccgtgattt ggtctgcatg tagcttgggc
     4561 gcgagacccg cggcacggcc agctgtcagt atccggcgcg cctcctccac cgtgaacgcg
     4621 ctgtcttcga cgaaaacgtc gcagcagcgc gccagccgtt ctcgaccggc ggctggtatg
     4681 atccgatcga ccacgaggtc cacataagcc gaccgacgat cccgatactc gggagcgacc
     4741 gtgtgggcgg ccagcaacgt cgggaccagc cgcatcggcc ccagctcggc aagcttctga
     4801 tacactcgaa ggatcttgag ctcggaggcc tcgtcgagcc cgtagccgct cttgcattcg
     4861 accgtggtca cacccagggc gaccatgtcg tcgaggacgc cggccccccg cttgagcaac
     4921 gcttcttccg aacatgcgcg ggttgcccgc accgtcgaga gaatgccacc tcccgacgcc
     4981 gcgatctcga gataactctc gcctcgaatg cgtcgctcga aatcgtcggc ccgccagcca
     5041 ccgaaggcca ggtgcgtgtg gcaatcgact agtcccggaa tcaccagccg accgccagca
     5101 tcgagcgtct cgccggcttc aaggtggcgg ggcagatctg attcgggacc ggcccagacc
     5161 acgcggccat cgtcccaggc cagcgcggcc cgaggtattg gatggatgtc ttgctggagg
     5221 ccttcggccc gacaggtggc gagcaggccg atgtttcgga gg
//
LOCUS       DASRJQ010000117         4598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_198835, whole genome shotgun sequence.
ACCESSION   DASRJQ010000117 DASRJQ010000000
VERSION     DASRJQ010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4598
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198835"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="VFB89_02740"
     CDS             <1..1073
                     /locus_tag="VFB89_02740"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZM26249.1"
                     /translation="GWIETQWRRHARVAPAELTALLGHRDPQVRQGILIAGEFGSADV
                     PALLARLADAETSVRAAACRALGLVGDPAAASALVERIQHGTGEERAEAAAALGRLPV
                     ESLGDLKGCLDPSAGPEAVVAALETVRVAQASQFEFEVLHLTRALSTPVRIAALRAAA
                     MLPGIRADQVLFRGLGDRDESVQVEALELLVQRGGDRTGATLTALLSAADSLRFRVIR
                     ALGRLRTAEAAPKLESLYAEAALHERVEIVRALTAIAPPGITDFLSVRLRAPEPEIRH
                     VAAFGFAQVAEPADLATLLEMVGHDDWALRNEAARALGRLRLPEGRASLLTLVRDIEP
                     VVARTARSALAEIANSSDSAAA"
     gene            1070..1912
                     /locus_tag="VFB89_02745"
     CDS             1070..1912
                     /locus_tag="VFB89_02745"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HZM26250.1"
                     /translation="MTPADEVECKLLSDLIQEQFGLCFAGVRQEILMSRVRGRLKALH
                     LGSLLDYYHFLRCHPKRDEELDKLACHLTNNETYFFREPHHFDIVVRHIVPALGDALR
                     ARPLRVLSAGCSSGEEPYSLAIALHDSGLDLRGIDWRIDACDLNRERVARAREGVYEG
                     GSLRACEPDNLRRCFEPQNGRYRLRDRYRKNVRFFEANLAAATGSIGAPPYDVVLCRN
                     LLIYFDEAGFDRTVRLLGRSLAPEGWLVLGHSESLIDRTDEFAPTWLEGAMIYRKRRI
                     EAAA"
     gene            1909..3015
                     /gene="cheB"
                     /locus_tag="VFB89_02750"
     CDS             1909..3015
                     /gene="cheB"
                     /locus_tag="VFB89_02750"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HZM26251.1"
                     /translation="MIRVFVVDDSAFARRAITRVLRAEPAITVVGEAASGPEALLRVP
                     SAHPDLVTLDLDMPGMNGLTVLRAMLGRDPGLRVLMLSAHTRDGAEATVEALAAGAVD
                     FLDKSRFGLMEFEGLGRELVARVKALGGRSLNGASAGGNGRHVPGEPGHALPISGSRG
                     ALGPLPPSPAHVAADLCVLGASTGGPAAIQQLLEVLPADFPLPVAVVQHMPPGFTGPF
                     ARRLDQRCRLRVREAVEGDRLERGRVFIAPAGSQMRLNANLGVMLSPETGQARHVPSV
                     DVLMLSAARVRPGRVLGILLTGMGDDGADGMAAIRAQGGVTLAESEATCVVYGMPRAA
                     YERGGVTHLLALPEIVEWLSDVKAGSLAESSARP"
     gene            complement(2975..4366)
                     /gene="mgtE"
                     /locus_tag="VFB89_02755"
     CDS             complement(2975..4366)
                     /gene="mgtE"
                     /locus_tag="VFB89_02755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HZM26252.1"
                     /translation="MNKSATPPTPELLYLLSGDEEDFAAACADFLPADIAEGLNGLPA
                     EAATRVVAALPFHRAVQVFDEPELTRRGELVAGLEPGRAIPLIEAMSSDQQVELFREL
                     SDEARGRLIHVLDAPTRDSLNLLLRYPPTSAGGIMTTEFVGVPATWNVEQVKTYIREV
                     GQAKETVYAIYVLDPKDQRLRRVVSLKEVMLADPGTSVLEVGETKKPVTVRPLTDQED
                     VARLISKYDLLAIPVVDDTSRVLGIVTVDDIIDALVSETTEDVQKFGGMEALDEPYMQ
                     ISLLAMVKKRAGWLCALFLGEMLTATAMGHFEAEISRAVVLALFIPLIISSGGNSGSQ
                     ATSLIIRAMALREVTLRDWWRVAVRELPSGLALGSILGVIGVARILLWQGIGWYDYGP
                     HYALVALTVGTALVGVVLFGSLAGSMLPFVLRRLGFDPASASAPFVATLVDVTGLVIY
                     FTVALTILRGTLL"
     gene            complement(4382..>4598)
                     /gene="cutA"
                     /locus_tag="VFB89_02760"
     CDS             complement(4382..>4598)
                     /gene="cutA"
                     /locus_tag="VFB89_02760"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HZM26253.1"
                     /translation="PIASTYRWQGQVERATEWYCHLKTTSVRLPDLESRLRSLHPYQV
                     PEIIAVPISAGHPAYLSWIEDAVRPTP"
BASE COUNT          740 a         1503 c         1549 g          806 t
ORIGIN      
        1 tcggatggat cgagacccaa tggcgccggc atgcccgcgt cgcgccggcc gagctgaccg
       61 ccttgcttgg gcatcgggac ccccaggttc gccagggcat tctgatcgct ggcgaatttg
      121 gctctgccga cgttccggcc ctgttggcgc gcctggccga cgccgagaca tcggttcgag
      181 cggcggcatg ccgtgcactc ggactggtcg gcgatcccgc ggcggcctcg gcgctggtcg
      241 agcggatcca gcacggcacg ggggaggagc gcgcggaagc agccgcggcg ctcggtcgcc
      301 ttccggtgga atcgctggga gatctgaaag gctgcctgga tcccagtgcc gggccggaag
      361 ctgtcgttgc ggctcttgaa accgttcggg tggcccaagc gagccagttc gagttcgagg
      421 tcctgcactt gacccgagcc ctatctactc cggttcggat tgcggccctc cgagcggcgg
      481 cgatgctgcc gggcatccgg gcggatcagg tcttgttccg cggcctcggc gatcgggatg
      541 aatcggtgca ggtagaagct ctcgagctcc tggtgcaacg tgggggcgac cgcacgggag
      601 cgacattgac ggccctcctc tccgccgccg attcgctgcg tttcagggtc attcgcgccc
      661 tcgggcgcct gcgtaccgcg gaggctgcgc ccaagctcga gagcctctac gccgaagccg
      721 ccctccacga acgcgtggag atcgtccggg cgcttactgc gatcgcgccg cccgggatca
      781 ccgattttct cagcgtgcgc ctccgcgcgc cggaacccga gatccggcac gttgccgcat
      841 tcggcttcgc tcaagtcgct gagcccgccg accttgccac gcttctcgag atggtaggcc
      901 acgacgactg ggcgctgcgc aatgaagccg cccgcgcact cggccggctt cgcttgcctg
      961 aaggtcgagc gtccctgctt accctggtcc gcgacatcga gccggtcgtt gcgcggacgg
     1021 ctcgcagcgc cctcgccgaa atagcgaaca gttccgactc ggccgcggca tgaccccggc
     1081 agacgaagtc gaatgcaaac tcctgtccga tctgatccag gagcagttcg gactatgctt
     1141 tgccggcgtg cgccaggaaa ttctgatgtc tcgcgtccgc gggcgcctga aggcgcttca
     1201 cctcggctcg ctgctcgact actaccattt cctgcggtgc caccccaaac gggacgaaga
     1261 gctcgataag ctcgcctgcc acctcaccaa caacgagacc tacttctttc gcgagccgca
     1321 tcacttcgac atcgtggtac gccacatcgt ccccgcgctg ggggacgccc tgcgagcacg
     1381 cccgcttcgc gtcctatccg ccggctgctc gtccggcgag gagccctact cgcttgccat
     1441 cgctttgcac gactccggtc tggaccttcg cggaatcgac tggcggatcg atgcgtgcga
     1501 cctgaatcgt gagcgagtcg cgcgtgcgcg agaaggcgtc tacgaagggg gctcgctgcg
     1561 tgcctgcgag ccggacaacc tgcgccgctg cttcgagccg cagaatgggc ggtaccggct
     1621 gcgggaccgg tatcgcaaga acgtccgttt cttcgaggcc aatctggctg ccgcgacggg
     1681 gagtatcggg gccccgccgt acgacgtggt gctctgccgc aacctgctga tctacttcga
     1741 cgaggccggc ttcgatcgga ccgttcgatt gctgggccgt tcgctcgcgc cggaaggctg
     1801 gctggtgctg ggccactccg agtccctcat cgatcgcacc gacgagtttg cgcctacctg
     1861 gctcgagggc gcgatgatct atcgcaagcg gcggatcgag gcggcagcgt gatccgtgtc
     1921 ttcgtggttg acgattccgc gttcgcccgg cgagcgatca cccgggtgct cagggccgag
     1981 cccgcgatca cggtcgtggg cgaggctgcg agcgggcccg aggctctgct gcgagtgccg
     2041 tcggcccatc ccgatctcgt cacgctcgat ctcgatatgc ccgggatgaa cggactcact
     2101 gtgcttcgcg ccatgctcgg ccgcgatccc gggctccgtg tcctgatgct gtcggcgcat
     2161 accagggatg gcgcggaagc gacggtcgag gcgttggccg ccggtgctgt ggactttctc
     2221 gacaagtcgc gattcggatt gatggaattc gaaggtctcg ggagagagct ggtggcgcgg
     2281 gtgaaggcgt tgggcggccg gtcccttaat ggagcgagtg ccggcgggaa cgggcgacac
     2341 gtcccagggg agcctggcca tgccctgcca atctcaggct cccgcggagc gcttggcccg
     2401 ctcccgccct cccccgccca cgtggccgcc gatctgtgcg ttctaggcgc gtcaaccggc
     2461 ggccctgctg ccattcagca gctgctggag gtgttgcccg ccgactttcc acttccggtg
     2521 gccgtcgtgc agcacatgcc acctggattt accggcccgt ttgctcggag actggatcag
     2581 cgctgccggc tgcgggtgcg tgaagccgta gaaggagatc ggctggagcg cggacgcgtg
     2641 ttcatcgcgc cggcaggaag tcagatgcgt ctgaatgcga atctgggcgt gatgctcagt
     2701 ccggaaaccg gacaggctcg ccacgtgccc agtgtagacg tgctgatgct gtcagcggcg
     2761 cgagtccgtc ctggccgcgt gcttggaatc ctgctcaccg ggatgggcga tgacggggcc
     2821 gacggcatgg cagcgatccg ggcgcagggc ggcgtcaccc tggccgaaag cgaagcgacc
     2881 tgcgtggtat atggcatgcc tcgcgctgcc tacgaacgcg gcggcgtgac ccacctcttg
     2941 gcgttgccag agattgtgga atggctctca gacgtcaaag cagggtccct cgcagaatcg
     3001 tcagcgcgac cgtgaagtag attaccagtc cggtcacgtc gaccagtgtg gcgacgaacg
     3061 gagccgatgc actggcaggg tcgaatccca gccggcgtaa cacgaacgga agcatcgatc
     3121 ccgccagcga tccgaacaga actactccga ccagcgcggt accgacggtc aaggccacca
     3181 gggcgtagtg cggtccgtag tcgtaccagc cgattccctg ccaaagaaga atccgcgcga
     3241 cgccgatgac ccccaggatg ctgccgagtg cgaggcccga cggcagctcg cgaacagcca
     3301 cccgccacca gtcacgcaac gtcacctccc ggagcgccat cgcgcggatg atgagcgagg
     3361 tcgcctggga tccggagttg ccccccgagc tgatgatgag tgggatgaac agcgccagca
     3421 ccacggcccg gctgatctcg gcctcgaaat ggcccatcgc cgtcgccgtg agcatctcgc
     3481 ccagaaagag cgcacagagc cagccggccc tcttcttcac catggcgagg aggctgatct
     3541 gcatgtaagg ctcgtccaga gcctccatgc ctccgaactt ctgcacgtcc tcagtcgtct
     3601 cgctgaccag ggcgtcgatg atgtcgtcca ccgtcacgat gccgagcact cgactggtgt
     3661 cgtcgaccac aggaatggcg aggagatcgt acttcgagat cagccgggcg acgtcttcct
     3721 ggtcggtgag cggccggacg gtgaccggct tcttggtctc gcccacttcg agcacggaag
     3781 ttccgggatc ggccagcatc acttccttga gcgagacaac ccggcgcagg cgctggtcct
     3841 tcgggtccag cacgtaaatg gcgtagaccg tttccttcgc ctgaccaacc tcacggatgt
     3901 aggtcttcac ctgctcgacg ttccaggtgg ccggaacacc cacgaactcc gtcgtcatga
     3961 tgccgccggc gctggtcggc ggatagcgga gcagcagatt gagcgagtcg cgcgtaggtg
     4021 catcgagcac gtggatcagg cggccccgcg cctcgtcgct cagctcgcgg aacagctcga
     4081 cctgctggtc cgatgacatc gcctcgatga gagggatggc ccgcccgggc tcgaggccgg
     4141 caaccagctc gccccggcgg gtgagctcgg gctcgtcaaa gacctggacc gcgcgatgga
     4201 atgggagtgc ggcgactact cgcgtcgctg cttcggccgg gagcccgttc agtccttcag
     4261 cgatgtcggc cggcaggaag tcggcacagg cggcggcgaa gtcttcctcg tcaccggaga
     4321 gcaggtagag gagctcgggg gtcggcgggg tcgcgctctt gttcatggag ccatcggcga
     4381 gctaaggcgt gggacgaaca gcgtcctcga tccaggagag ataggccgga tggcccgcgc
     4441 tgatcgggac ggcgatgatt tcggggacct gatatgggtg caacgagcgg agtcggcttt
     4501 ccagatccgg cagccggact gaggtggtct tgaggtgaca gtaccactcg gtggcgcgtt
     4561 ccacttgacc ttgccagcga taggtgctgg cgatcggg
//
LOCUS       DASRJQ010000118         3489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_483881, whole genome shotgun sequence.
ACCESSION   DASRJQ010000118 DASRJQ010000000
VERSION     DASRJQ010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483881"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="VFB89_02765"
     CDS             <1..880
                     /locus_tag="VFB89_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26254.1"
                     /translation="PDGIVDGLRANGLSRLALAPCVIFNGACYTGVTARWFDVTNADG
                     LVHEQIIPPEKSFSLGILDTPVVGYLAALHPDHGLPVYQEMEFLATTGGSLGDAIKST
                     YDGIVLANGGRLPIFERLENRKQAPRWTPSEVMLKGTASRILFGDPSLVPFAAFAKDP
                     LETTCTDKDNALRITASVVHPELLNSLTDTYHSDLSTDRNLFNARVLLSVRLPEDWKT
                     VAQVKTTSASVARSFLQRLGRQEAQKIQHRVVGFAVEEEGAGRSLRVQVDLSSTGYRE
                     SPFLRVGSVLELEATR"
     gene            922..1410
                     /locus_tag="VFB89_02770"
     CDS             922..1410
                     /locus_tag="VFB89_02770"
                     /inference="COORDINATES: protein motif:HMM:NF020177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthrone oxygenase family protein"
                     /protein_id="HZM26255.1"
                     /translation="MATMDHWLFGLTLVTAIGSGLMAGVFFAFSTFVMRALARLPAHE
                     GIAAMQSINVAVINPVFLGGFLGTAVACAAEIVCAGVCWQRSGAAYLLAGAALYLAGT
                     FFVTMRFNVPLNNSLAAISPSGPDAAEHWASYVRRWTAWNHVRTIAAAAASAAFTIAL
                     GR"
     gene            1586..2413
                     /locus_tag="VFB89_02775"
     CDS             1586..2413
                     /locus_tag="VFB89_02775"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HZM26256.1"
                     /translation="MSARKRVWIASVSGAALAGLLAASLVSTAAKAVAEKPAGNDHHG
                     SLPSQEELQAALAKVVQDGGNGGLGLNMWGTIVNRDGVVVAVAFTGGDRGDQWPGSRV
                     ISAQKANTANAFSLPGLALSTANLFSAVQPGGSLFGLQESNPVDTSVAYRGNSRFYGQ
                     VNDPMVGRKIGGVNVFGGGLALYTSEGELVGAIGVSGDTSCTDHVIAWKVRHALNLDN
                     VPAGVSATSDDNIIHDIAEDANGHAKSEKGFGHPVCDDGAADIASHLPETHPIGPAE"
     gene            2781..>3489
                     /locus_tag="VFB89_02780"
     CDS             2781..>3489
                     /locus_tag="VFB89_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26257.1"
                     /translation="MRIPWSGLAVLAAVVGGVSPASVSLRAQAPPAPCPTTEVTFFLA
                     SPQAQDGALELVAYTGEALEIGVIAHIRSSSSEPDDWVQSFALSVAHDREALTLLSAA
                     LNMDEVPELSVGGAINGVHPVENATGAGFVGHHVGLCGGETACWLPAVSDSPVVEARY
                     GIVLPPRDHEPVGPPVRIETTIEFRDGLQGPGQPILNAVTLHGATEVPCMEHLTLRIE
                     VLESREFVRGDANFDQRI"
BASE COUNT          617 a         1120 c         1116 g          636 t
ORIGIN      
        1 ccccgacggg atcgtggatg gactacgcgc caacggcctt tctcgtctgg cccttgcgcc
       61 ctgcgtgatt ttcaacggcg catgttacac gggggtcacc gcccgctggt tcgacgtgac
      121 gaacgccgat ggcctcgtgc acgagcagat catcccgccc gaaaagagct tctccctcgg
      181 gatactcgac acccccgtcg tgggatacct cgctgcgctc caccccgatc acgggctgcc
      241 ggtctaccag gaaatggagt tcctcgccac gacgggtgga tccctgggcg acgcgatcaa
      301 gtcgacctac gatggaattg tgctcgcgaa cggagggcgg ctacctatct tcgagcggct
      361 ggagaacagg aagcaggccc cgcgatggac accgagcgaa gtgatgctga agggaaccgc
      421 ttcacggatt ctgttcggcg atccttcgct cgttcccttc gcggcgttcg cgaaggaccc
      481 gctcgagaca acctgcactg ataaggacaa cgcgctccgc atcaccgcga gcgtcgtcca
      541 tccagagctc ttgaactcgc tcaccgacac gtaccattcg gacctctcca ccgaccggaa
      601 tctcttcaac gcgcgtgtgc tattgtccgt gcgacttcca gaggattgga aaaccgttgc
      661 gcaggtcaag accacttcgg catcagtggc gcgatcgttt ttgcagaggc tcgggcggca
      721 ggaggcgcag aaaatccagc accgcgtagt tggtttcgct gtcgaggaag aaggcgccgg
      781 ccggagcctt cgggtgcagg tagatctctc atccacgggg taccgagagt cgccgttcct
      841 gcgagtgggc tcggtcctcg agctggaagc gacgcgctga atccgaaaag ctttcgcaag
      901 atgggaggcg cgggaagctg aatggcgacg atggaccact ggcttttcgg tctgacgctc
      961 gttaccgcga ttggctcggg gctgatggcc ggcgtctttt ttgctttctc gacgttcgtc
     1021 atgagagccc tcgcccgctt gccggcccac gaaggtatcg cggccatgca gtcgatcaac
     1081 gtcgcggtga tcaaccccgt gtttctcggc ggcttcctcg ggacggccgt cgcctgcgcg
     1141 gccgaaatcg tctgcgccgg ggtgtgctgg cagcgatcgg gggcagccta cttgctcgcc
     1201 ggcgcagcgc tctacctcgc cgggacgttt ttcgtgacga tgcggttcaa cgtgccgctc
     1261 aacaactctt tggcagcgat ctcgccgtca ggccccgacg ctgccgagca ttgggcgtcc
     1321 tacgtccgca ggtggacggc atggaatcac gtgcgcacga tcgcagcggc cgcggcatct
     1381 gcggcgttca ccatcgcgct cggtcgctga cgctgctctc gcgacagcgc ggaattcccg
     1441 tccgaccaac agattcctaa cagcttgact tcgatgttgg cgtatgcggc aatcccgcac
     1501 tctgttgagg gttcttgcgt ttcgggccat tgggggccgt ggaaagcgag agcctattcc
     1561 gcgccctgga aaggagattg gaaaaatgag tgcacgaaag cgcgtttgga ttgcttcggt
     1621 ttctggagcg gcattggctg gactcctcgc tgcatcgctc gtttcgactg cggcgaaggc
     1681 cgtcgctgag aagcccgcag gcaatgacca ccacggaagt ctgccgagcc aggaagagct
     1741 gcaggcggct ctagccaagg ttgtccaaga cggcgggaac ggcgggctcg gcctcaacat
     1801 gtggggcacg attgtgaatc gcgacggcgt cgtcgtcgcc gtggcgttca ccggcggcga
     1861 ccgcggcgac cagtggcccg gcagtcgggt catctccgcc cagaaggcga atacggccaa
     1921 cgcgttcagc cttcccggcc tcgcgctctc cacggccaac ctcttctccg cagtgcagcc
     1981 gggtggatcg ctcttcggcc tccaggagtc gaaccccgtg gacacgagcg tcgcgtaccg
     2041 cgggaactcg aggttctatg gtcaagtcaa cgatcccatg gtcggcagaa aaatcggcgg
     2101 agtgaacgtg ttcggcggcg ggctcgcgct gtacacgagc gagggcgagc tcgtgggcgc
     2161 catcggcgtg agtggtgata cttcctgcac cgatcacgtc atcgcttgga aggtccgcca
     2221 cgctctcaac ctggacaatg tgccggcggg tgtgagcgcc acgagcgatg acaatatcat
     2281 tcacgacatt gccgaggatg cgaacggcca tgcgaagagc gagaagggct tcgggcaccc
     2341 ggtctgcgac gatggagccg ccgacatagc gagccatctc cccgagacgc acccgatcgg
     2401 cccggcggag tgaccgaaga cggcgaattt aagttataag acgcctgcga agttcgagtt
     2461 tcgccatttt cggcgatcct ggcgctaacc gcagggacgg acggttttga cgtaggcgcc
     2521 tccgggccca gccttgcaga aatagcccag cttaccgacg tcaccgcgaa ttcaggccgt
     2581 ccaggccggc gaaaactggc gaaactcgaa cgaaagtccc ggagcagcga actgcgctgg
     2641 tccgggacct tcgactcggc ggggcacgag cgagtcccgt cggctcgaaa ccttgcatcc
     2701 cgaccgagcc gaccatcaat ctctgctcgc gccgccgcgg tggaagagct atcctttgtc
     2761 gcgcagggga ggttaaagcg atgcgcatac cctggtcggg cttggcggtg ttggcggcag
     2821 tcgtcggtgg ggtctcgcct gcttccgtct cactccgcgc gcaggcgcca cccgcgccct
     2881 gccccacgac ggaagtgact ttcttcctgg catcacccca ggcccaggac ggcgcgctcg
     2941 agctcgtggc gtacactggc gaggctctcg agatcggcgt catcgcacac attcgctcga
     3001 gctcctcgga accggatgac tgggtccaga gcttcgccct gtcggtggcg cacgaccggg
     3061 aggcgctcac cctgctctct gccgcgctga acatggacga agtcccggag ctctcggtgg
     3121 gcggcgccat caacggggtc catcccgtcg aaaacgccac cggcgcagga tttgtcggcc
     3181 accatgtcgg cctctgcggc ggtgaaaccg cttgctggct gcccgccgta agcgacagcc
     3241 ccgtggtgga ggcacgatat gggatcgtcc tcccacctcg cgatcacgag ccggtcggac
     3301 ctccggtccg catcgagaca accatcgagt tccgcgacgg cctccagggc ccggggcagc
     3361 ccattctgaa cgcggtcacc ctgcacgggg ccaccgaagt cccgtgcatg gagcacctga
     3421 ctctccggat tgaggttctg gagagccgcg agttcgtccg cggagacgcc aactttgacc
     3481 agcggatcg
//
LOCUS       DASRJQ010000119         5411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1610454, whole genome shotgun sequence.
ACCESSION   DASRJQ010000119 DASRJQ010000000
VERSION     DASRJQ010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5411
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610454"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..505
                     /locus_tag="VFB89_02785"
     CDS             242..505
                     /locus_tag="VFB89_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26258.1"
                     /translation="MYPSQMVPLPNGRALVIERAAHGAASTDEVVIWISRDQVVPEGG
                     VIPTRDRAIGEGIRFPQAALPDINVALAHFHRLSSRSRQHVEG"
     gene            complement(927..1385)
                     /locus_tag="VFB89_02790"
     CDS             complement(927..1385)
                     /locus_tag="VFB89_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26259.1"
                     /translation="MIDSASLFRDVWAWSLNNAEPWHNWVAHGLIAAILGILIGVIAK
                     ALGVPFGVGPWTAASYYVLREGEELMLAWGFPGSIDWKDHALDAGVPVVCSVIVYLVS
                     LLVRVRIVPPRRSGWRGPPATQKASPKSALLHDRGMTRRASNNPGHRGER"
     gene            1483..2223
                     /locus_tag="VFB89_02795"
     CDS             1483..2223
                     /locus_tag="VFB89_02795"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZM26260.1"
                     /translation="MSGVPRKGTVMYRLAKIIPRGLRRRLEVFLIDRGFPTKKQRGMR
                     GIEQLGHRGYVGRREWYEADGLRQFEFMLEHGLQPGDVLCDIGCGSLRGGRLFIEYLD
                     RGNYLGLDSEEELVELGLRHELDPRVREDKAPEFVISYGFEFRRFSKPPTRALAVSLF
                     SHLNERDIRLCLRNLAHHATGPCQLFASYFESERPTPNFQKSHPQLAFYYTREQMQRM
                     GHDAGWTPAFLGEWGSPAGQLMIRFTKE"
     gene            complement(2249..4084)
                     /locus_tag="VFB89_02800"
     CDS             complement(2249..4084)
                     /locus_tag="VFB89_02800"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HZM26261.1"
                     /translation="MASPTTALRVERVGSLLELAIWAGLVTGVAELAKVGLDLFYSDF
                     TDRSRDALWMIPAVDAGLFAAVGLVLLAIQVWVRVPWNVAAGLFLGLGTGLVLLLVQR
                     LHPAAAVLLAAGIGTQASRLVVKRITLARRLIHRSVIWLTALVGLIWLLMVGWRAAGQ
                     RVSMHGHRGLPLGSPNVLLLILDTVRAADLSLYGYARPTTPELERLAERGTVFDRAFA
                     PAPWTLPSHASIFTGRGALELGVNSHIRLDKRWLTLAEALQARGYATAGFVANTDYAG
                     WESGLDQGFGYYDDYPVTFATALKATAFGSVFYPALRELGAPVFKRLRVSWLPKLRRH
                     RSAPQITDSFLAWLDRPRQKPFFAFLNFMDAHDYTAPEPFRSKFRSSSPRRTSRWAWA
                     DSPPVRLTPADLRPRQDAYDGAIAYLDSEIGKLFSELERRDLLERTLVIVTSDHGEEF
                     GEHGLASHGHSLYRPSLQVPLAIWLPGYVAEGRRVTAPVSLQDIPATVLELADSSRLT
                     PLPGRSLSRFWTGQDTSSGTLISGVGSEPKLPNWYPVARGNLRSLAFDGWKYIRNDGD
                     GTEELYDFEHDLLERWNLIGTGEGNRLLPRYRNAAAQQAAGEGAQ"
     gene            complement(4090..4428)
                     /locus_tag="VFB89_02805"
     CDS             complement(4090..4428)
                     /locus_tag="VFB89_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26262.1"
                     /translation="MSDIPVACLLTVPELLQRRNTVLAAFRAAQLEVRELAEAKAEGF
                     AFRVAAGAQLSALAELIELERQCCPFLRFRLTVGPGDAPIWLTVTGPAGTREFLLHEL
                     GFVRDPNDPG"
     gene            complement(4461..4868)
                     /locus_tag="VFB89_02810"
     CDS             complement(4461..4868)
                     /locus_tag="VFB89_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZM26263.1"
                     /translation="MSNRKGFTLIELMIVIVIIGILAAIAIPKFSNTKQKAVRSSMIS
                     DLKSLAGAQEAFYSDSTRYAGSYSNTRGSNSVNFSPSQNNVVSITTGGTGSYWSATVT
                     NTGYNSGSSVCGIFVGTTATVSGATTEGVPGCQ"
     gene            5108..>5411
                     /locus_tag="VFB89_02815"
     CDS             5108..>5411
                     /locus_tag="VFB89_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26264.1"
                     /translation="MSLTYSIDPVAGVVELRYSGGYDFEEWSSVMHAILRDPAFSAGY
                     GFLSDRRLVSEAPTTDFVQGTLSFLRTHQALLRATRWAAIVSDAASYGMGRMAQQLG"
BASE COUNT         1073 a         1678 c         1711 g          949 t
ORIGIN      
        1 cgagcgagga gcgcgcttgc tggccaacgc aagcgccggg aagcgtgttt ctcgcaggga
       61 cgggatgatg gcgtgccggc gatggccgaa aaaggcaccg ctctgcgtct aattcgggct
      121 agcagtgcag ctaccaggcg tcaacaacga tagccgcggc cggagcgctc gggctgctgc
      181 tcgctgaggc acgccgacgg acaattcgtt taacgagaag gtcaagagga gcgacttcac
      241 gatgtaccca tcacaaatgg tgccattgcc gaatggccgg gcactcgtga tcgagcgggc
      301 cgcgcatggc gctgcgtcga ccgacgaggt ggtcatttgg atttcgcggg accaagttgt
      361 gcctgagggc ggcgttattc cgacccgtga tcgcgcaatc ggggaaggca ttcgtttccc
      421 gcaggccgcg ctgcctgaca tcaacgtggc gcttgcccac tttcaccgct tgagcagtcg
      481 ctcccgccag cacgtggagg gatgagacct tgggattcgc agtggccgga ctattcatgg
      541 atcgggcgcg agggggtctt cgagctgatt atgcttctga aagccacgag cgcgcgctca
      601 gtaaggcgct caccccaggc gcttaacgga gtgtggcgca cgaaaggcag gatgctggca
      661 gggaactgtg agtcgaatga tcgggccttt agcaggaccc gagctggagg acgctctccg
      721 gcacctgatc cgcgacctca gggtgattga gggtgcgatc cagcgggtga acgtggcagt
      781 agccccccag gcgctgtggt ctcttcgcgg gctctcgcac cagcttgaca gcgtaagacc
      841 acgctatgcc gcgtgtctcg cgagcattac gcagcaggct ctaaatcccg aacggtcggg
      901 taacggcggg gcacacgcgg ggggtcttat cgctctcctc gatgtccggg attattggac
      961 gcgcgtcgag tcatccctcg atcgtgcagc agcgcagact ttgggctggc tttctgcgtg
     1021 gctggcggcc ctcgccatcc tgatctcctt ggtggcacta ttctcacgcg tacaaggaga
     1081 ctgaccaggt acactatgac cgaacagacg actggaaccc ctgcatcaag tgcgtggtcc
     1141 ttccagtcga tggagccggg gaagccccac gccagcatca gctcctcgcc ctcacggagc
     1201 acatagtagg acgccgcagt ccacggcccc acgccgaacg ggacgcctaa tgccttggct
     1261 ataacaccga tcaggattcc gaggatcgcc gcgataaggc cgtgcgcgac ccagttgtgc
     1321 cacggctcgg cattgttgag cgaccacgcc catacgtcgc ggaatagact agccgagtcg
     1381 atcatcaaaa ctcaccacgc cgcacactcc tcgataccga cgaagaccct ccgactaagc
     1441 gcccggtcag cctacctagg gggtgagaat tcctgttgat ttatgagcgg cgtcccacgg
     1501 aagggaaccg tgatgtatcg tctggcaaag atcatcccga gggggctcag gcggcgcctc
     1561 gaagtgttcc tgatcgaccg cgggttcccg acgaagaagc agcgggggat gcggggaatc
     1621 gagcagctgg gccaccgggg atacgtcggc aggagggaat ggtacgaggc cgacggccta
     1681 cgtcagttcg agttcatgct tgagcatggg ctgcagcccg gcgacgtgct gtgcgacatc
     1741 ggctgcggct ctctgcgcgg cgggcgcttg ttcatcgagt acctcgaccg cgggaactac
     1801 ctcggcctcg atagcgagga ggagctcgtc gagctcgggc tacgacacga gctggacccg
     1861 cgcgtgcgag aggacaaggc tccagagttc gtaatctcgt acgggttcga gtttcgccgc
     1921 ttctcgaaac ccccgacgcg cgcgttggct gtgtccctgt tcagccacct gaacgaacgc
     1981 gacatccgac tctgtctccg aaatctcgcc caccacgcga cgggcccctg tcagctcttc
     2041 gcgtcctatt tcgagtcgga gcggccgacg ccgaacttcc agaagtcaca cccccagctc
     2101 gcgttctact acacgcgcga gcagatgcaa cggatggggc acgatgccgg atggaccccg
     2161 gcgttcctgg gcgagtgggg atcgccagcg ggccagctga tgatccggtt tacgaaggag
     2221 tagcgtaggc cgaggggagc gcgagggact actgcgctcc ctcacctgct gcctgctgcg
     2281 cggctgcgtt gcggtagcgc ggcagcagcc gattaccctc gccggttccg atcagattcc
     2341 aacgctctaa caaatcgtgc tcaaaatcgt aaagctcctc ggtcccgtcg ccgtcgttgc
     2401 ggatgtactt ccatccatca aatgcgagag accggagatt gccccgagcc accgggtacc
     2461 agttaggcag ttttggctcg gatccgacgc cggagatcaa ggttccggag gacgtgtcct
     2521 gacccgtcca gaagcgcgaa agggagcggc cgggcagggg tgtcagtcga ctggagtccg
     2581 ctagctcgag caccgtcgct gggatatcct gaagagaaac tggtgcagtc acccgccgcc
     2641 cctctgctac atatcccgga agccaaatcg cgaggggtac ctggagggac ggccggtaaa
     2701 ggctgtgtcc atggctcgcc agaccgtgct cgccgaactc ctccccgtga tcggaggtga
     2761 cgatcaccag cgtgcgctcg agcaaatccc ggcgctcgag ctcactgaaa agtttcccaa
     2821 tttcagagtc gagatacgca atggcgccgt cgtaggcatc ctgcctgggt cgtaggtctg
     2881 ccggcgtcaa gcgaacagga ggagagtccg cccaggccca ccgcgaggta cggcgcggac
     2941 tggaagagcg gaatttgctg cggaatggct ccggtgccgt gtaatcgtgg gcgtccatga
     3001 agttgagaaa cgcaaagaac ggtttctgcc ggggccggtc gagccaggca agaaacgaat
     3061 ccgtgatctg cggagctgaa cgatgtcgcc gcagctttgg gagccaggat actctcaacc
     3121 gcttgaaaac cggcgctccg agctcccgca aggcagggta aaagacactt ccaaacgcgg
     3181 tcgccttcaa ggccgttgca aacgttaccg gatagtcgtc gtagtaaccg aacccttggt
     3241 caagcccgct ttcccagcca gcgtagtccg tgttcgccac aaaccccgcc gtagcatatc
     3301 cccgggcctg cagcgcctcc gcgagggtca gccaacgctt gtccagtcga atgtgcgagt
     3361 tcacgccgag ttcgagcgcg cctctgccag tgaagatcga ggcgtgggat ggaagggtcc
     3421 atggggccgg ggcgaaggct cggtcgaaca ccgtgccgcg ctctgccagg cgctcgagct
     3481 ccggcgtagt cggacgagcg tacccgtaca ggcttaaatc ggccgcccgc acggtgtcga
     3541 ggataaggag gagaacgttc ggggaaccaa gggggaggcc ccggtggcca tgcatcgaaa
     3601 cgcgctgacc tgctgcgcgc catcctacca tgagaagcca gatcaggccc accaatgcag
     3661 ttaaccagat cacgctccga tgtatcaggc gcctcgccag agtgatccgc ttaacgacca
     3721 ggcgcgaagc ctgagtccct atcccggccg ccaatagcac tgcggcagcc ggatggaggc
     3781 gctgaaccag caacaggacc aagcccgtgc ccaagccgag gaaaagaccc gcggcgacat
     3841 tccacggcac ccggacccag acttggattg ccagtagcac cagccccact gccgcgaaca
     3901 ggccggcatc cactgccggt atcatccaga gcgcgtcgcg gcttcgatcc gtgaagtccg
     3961 agtagaaaag gtcgaggcct acctttgcga gctcagccac gcccgtgaca aggcctgccc
     4021 agatcgcaag ttcgagcagc gaccccacac gctcgaccct gagcgctgta gttggcgatg
     4081 ccatgtccgc taacccgggt cgttcggatc tcgcacgaag cccagctcgt gaagcaggaa
     4141 ctcccgggtg ccggcggggc cggtgacggt caaccagatc ggagcatcac ccggccccac
     4201 ggtaagcctg aagcgcagga aggggcagca ctgacgctcg agctcgatca gctcggccaa
     4261 tgcggagagc tgcgcgcctg cggcgacgcg aaatgcgaac ccctccgcct ttgcctcggc
     4321 cagctctctg acctcgagtt gagccgcgcg gaaagcggcc aacaccgtgt ttcgacgctg
     4381 tagcagctca ggcacagtca ggaggcaggc aacagggatg tccgacattg ggtgagctca
     4441 ggtagcgcac tcgcccgggc ttactgacaa cccggcacgc cctcggtcgt agcaccggac
     4501 accgtcgcag tcgtgccgac gaagatgccg cagacggacg acccgctgtt gtagccggtg
     4561 ttggtcaccg tagccgacca gtacgagccg gtcccgcccg tggtgatcga cactacgttg
     4621 ttctggctgg gcgagaagtt caccgagttt gagcctcggg tgttgctgta gctaccggcg
     4681 tagcgagtcg agtctgagta gaacgcctcc tgcgcgccag ccaggctctt gaggtccgag
     4741 atcatgctgg agcggaccgc cttctgcttg gtgttcgaaa attttggaat ggcgatcgcc
     4801 gccagaatgc cgatgatcac gatcacgatc atcagctcga tcagggtaaa gcccttgcga
     4861 ttcgacatgt ccgatctcca gcatggcctg acggcagagc gcaggctccg gaaggataga
     4921 ggcaccgaag cccccaccgt tgcacgctgg aaggtgcgat caacgcctgt tattcgcaaa
     4981 tagccccagc cacgcacgat ggcggggaga gccttgaact gcgcgacact atatcttcca
     5041 acttagttcc ggtttcaagc agcgcaccag gagtccaggg gttactctcg aacggagata
     5101 gcctcgcatg agtctcacct attcgatcga cccggtcgct ggtgtcgtgg agctccgcta
     5161 ctcgggaggt tacgattttg aagagtggtc gtcagtcatg catgcgattc tgcgggatcc
     5221 tgctttcagc gccggctacg gctttctcag tgatcgcaga ctggtgtcgg aagcacctac
     5281 caccgacttc gtgcagggca cgctcagttt tctgagaacc caccaggccc tgctccgggc
     5341 gactcgctgg gccgccatcg tcagcgatgc tgcttcctac ggaatgggcc ggatggccca
     5401 gcagctcgga g
//
LOCUS       DASRJQ010000120         4820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1369608, whole genome shotgun sequence.
ACCESSION   DASRJQ010000120 DASRJQ010000000
VERSION     DASRJQ010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369608"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1496..4579
                     /locus_tag="VFB89_02820"
     CDS             1496..4579
                     /locus_tag="VFB89_02820"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZM26265.1"
                     /translation="MTVLFADLKGSMELLADRDPEEARKLLDPVLERMMEAVHRYEGT
                     VNQVMGDGIMALFGAPVAHEDHGVRACYAALRMQASVKQYAEEIHRTEGVPIQIRVGL
                     NSGEVVVRSIGSDLHMDYTAVGQTTHIAARLEQMTMPGSVLISPATLALAEGYVMVKP
                     LGPRPIRGLELPLEVYELIGAGSVRSRFQAAAARGLTRFVGRESELDQLRQALERARA
                     GHGQVAAVVGEPGVGKSRLYWEFSHSHRAQGWLIIESGSVSYGKATNYLPIVELLRAS
                     FSIESADDPRKIREKVTGKLLSLDRQLEPWLVPILWLLDVPVADEHWERLDPPQRRQR
                     ILEGVKRLLLRESQVQPLMVIFEDLHWVDAETQAFLDNLVESLPTARLLLLVNYRPEY
                     SHGWGSKTYYRQFQVDPLAPASAEALLDVLLGSDATLAPLKRLVIERTEGSPFFIEET
                     VRSLVETGVLTGERGAYRSTRVVQGLQVPATAQAMLAARIDRLTPEDKRLLQAAAVIG
                     TDVPFVLLQAVADEPEDRLRRGLAQLQATEFLYETRLFPDLEYTFRHALTHEVAYGSL
                     LAERRRALHARIVEAIERLYPDRLTELVERLAHHAVRAEAWEKAVTYLRQAGARTFAR
                     SANREAVGYFEQALVALQHLPATRETLEQAIDLRFDLRPALFALGDLPRLAEHLHDAE
                     ALAHTLGDQLRLGRVSLHKSHYLWSTGHAAEARASAENAQAIAEALGDLPVQVGATHY
                     LGAICLTSGNYRHAETFLRKVVDLLSGDRSRDRCGLTGFPAAMARGWLAWVLAERGAF
                     DEGIVYGQDGLRLAEALDHPYSWIAASWGPAHLYRLRGEIGHAVRLLERALSLCRDWD
                     ILVLSGTTAGHLGFVYVRSGRVAEGLALLSEGQESEKQYQTLIIAHMGEACLLAGRVD
                     EAITFADRALTFARERGQRGYEAYALRLLGEIAAHPDSPDAEAAEGYWRQAMALADEL
                     GMRPLVAHCHLGLGKLYRRTGKREQAQEHLTTATTMYREMGMTYWLEQAEAEMND"
BASE COUNT          823 a         1650 c         1546 g          801 t
ORIGIN      
        1 cacgcgcaaa aatttcaaaa gagccaccct atagtcagca cggacactca ctcgctcgcg
       61 ggccagtgtc cgcttcgctc gttcggtcaa tagcctgcta tccgggtctc aagttttgat
      121 gtgacgcttg ctcgctctta ggcccgtgcg gatctctttc cgcgctctgc cgctacgggc
      181 ctcgctccac tagagcgctc cacctaacac ctctcacacc tcacaccact cgaaggagtt
      241 cacgtcatgt cccatcgccc ccaccgttta tcctctgccg tcccccagcc gccgtccgcc
      301 tccacccccg gagagcaggc cacggctatt tctgtctctc gccaaccaac catccggtag
      361 ctcgtctgtt ccagtccgct ctgcggcacg cggtcggcgg agagtcgccg acgcaaaccc
      421 ggctcgcggt gcggcgacat gcaccgcggc tggttcggcg actgcgacgg gcgcctcgtc
      481 gccggggatg caggaggtgc gcgatgacgc ctgcgctctc aatccctcag accatcacca
      541 tctatcgaca gaagcatccc gaggggatga tctgtcccga gtgcgggagg ctgctggcca
      601 cgcggcgcga gagctacgcc aagtccaacc tgattgaagc ccggcgcctg gagtacgtgt
      661 gcgccgagtg tcggctggat cgggcggagg ctgagcgtat ccacgcagcc agggtcgaga
      721 gggccaagat cgcggcccaa gcgtccgctg aggcccgccg tcgccaagag tcctcatctg
      781 tagatagaaa ccccctccct gtgggagaac ccgcgcttgg cgattctggg gctccgcctg
      841 tctcgaaccc cgcggtttat gcgggtgccg agggtgggtt tctatctaca cgcagtcaac
      901 cggccgtcat ttcccagcgc cggggcggac ggcctcgaaa gcaccctacc aaccgggccg
      961 cccggaccgc agcccagcag gcatggcgag cacggaagcg gcagcgctcc gtgccaagtg
     1021 aggtgcccgg caatgccttt tgagatcttc gacatctaca ccgcgggtac tgcctgatgc
     1081 gcggttacat gtcacggtgc gcgcctgcgc ccagccgcgt agcgttagtc tttgttagcc
     1141 gatcctgcgc cacagatggg cccgccagtt gaccatgcac caggcaacag tctaggctcg
     1201 ccccagctaa tcgccccgac tctggaggtt ccgcgtgacg tgtccccggt gtcagcacga
     1261 gaatcgttcc ggggcgaagt tctgtgaaga gtgcgcgacc ccgctggcgc ggagttgctc
     1321 gaactgtgga agccaactat cgaccacggc gaagttttgt ccggagtgcg cccatcccgt
     1381 agccgggaca actcccgcag aagctcgctt cgcctcaccg cacagctata cccccaagca
     1441 cctcgccgag aagatcctca cctccaggag ctcgcttgag ggtgagcgga agcaggtgac
     1501 ggtgctgttc gcagatctca agggttccat ggagctgctg gctgaccggg atcccgagga
     1561 agcacggaag ctccttgacc ccgtgctcga gcggatgatg gaggccgtcc accgctacga
     1621 gggcacggtt aatcaggtaa tgggtgacgg catcatggcg ctcttcgggg cgccggtggc
     1681 ccatgaggat cacggggtgc gggcctgtta cgccgcgctt cgaatgcaag cgtcggtcaa
     1741 gcagtacgcc gaggagatcc accggacgga aggcgttccc atccagattc gcgtcggtct
     1801 gaactccggc gaggtcgtcg tgcgctccat tggtagcgat ctgcacatgg actacaccgc
     1861 ggtcggccag acaacccaca tcgcggcccg gctggagcag atgacgatgc cgggctcggt
     1921 gctgatctcg ccagcgacgc tcgcgctggc cgaggggtac gtcatggtga aacccctcgg
     1981 gccacggccg atcaggggcc tcgagctgcc gctcgaggtc tacgaactga tcggagccgg
     2041 ctccgtgcgc tcgcgcttcc aggccgcagc cgcccgcggc ctcacccgct tcgtgggccg
     2101 agagagcgaa cttgatcaac tccgccaggc gctcgagcgc gcgagggccg gtcacgggca
     2161 agtggcggcc gtcgtggggg agccgggcgt tgggaagtcc cggctctact gggagttcag
     2221 tcactcccat cgcgcccagg gctggctgat catcgaatcg ggctccgtct cctacggcaa
     2281 ggccacgaac tacctgccca tcgtcgagct gctccgggcg tccttttcga tcgagagcgc
     2341 ggatgatccc cgcaagatcc gcgagaaggt gacgggcaag ctgctgtcgc tcgaccggca
     2401 gctcgagccc tggctggtgc cgatcctgtg gctgctcgac gtcccggtcg cggacgagca
     2461 ctgggagcgc ctcgatccgc cccagcgccg acagcgcatc ctcgaagggg tcaagcgcct
     2521 gctcctgcgc gagagtcagg tgcagccgct catggtcatc ttcgaggatc tgcactgggt
     2581 cgacgccgaa acccaagcgt tcctagacaa cctggtggag agcctaccca cggcccgact
     2641 gctgctcctc gtgaactacc gcccagagta cagccacggc tgggggagca agacctacta
     2701 ccgccaattc caagttgacc cattggcgcc tgccagcgcc gaggcattgc tcgacgtcct
     2761 cctgggcagc gacgccacgc tcgccccgct caagcgcctg gtgatcgagc ggactgaggg
     2821 cagtccattc ttcatcgagg agaccgtccg gtcgctggtc gagacgggag tcctgacggg
     2881 cgagcgtgga gcctaccgct cgacccgggt cgtccagggc ctccaggtcc cggccacggc
     2941 ccaggcgatg ctggcggcgc ggattgatcg gctgaccccg gaggacaagc gactcctcca
     3001 ggcggccgcc gtcatcggca ccgatgtccc ctttgtcctg ctccaagctg tggccgacga
     3061 gccggaggat cggctgcgcc gcgggctggc ccagcttcag gcgaccgagt ttctctacga
     3121 gacgcggttg ttcccggacc tcgagtatac gttccggcac gcgctcaccc acgaggtcgc
     3181 ctacggcagc ctcctcgcgg agcgacggcg tgccctccat gctcggattg tggaggccat
     3241 cgaacggctc taccccgacc gcctcactga gctcgtggag cggctcgccc accacgcggt
     3301 ccgggccgag gcgtgggaga aggccgtgac ctacctccgt caagccgggg ccaggacgtt
     3361 cgcgcgatcg gcgaatagag aagcggtcgg atacttcgag caggcgctgg tggccctcca
     3421 gcatctgccc gcgacccgcg agacactcga gcaggccatc gatctgcgct tcgaccttcg
     3481 acccgcgctc ttcgccctcg gcgacctccc gaggctcgcc gagcatctgc acgacgccga
     3541 ggccctcgcg cacacgctcg gcgatcaact gcgactcggg cgggtatcgc tccacaagag
     3601 ccactatctc tggagcactg gccacgcggc tgaagcgcgt gcatccgccg agaacgccca
     3661 ggctatcgcc gaggcgctcg gagatctccc ggtccaggtt ggggcgactc actatctcgg
     3721 cgcaatctgc ctcacctccg gcaattatcg gcacgccgaa acgttcctcc gaaaggtcgt
     3781 ggacctgctc agcggcgatc gcagccgtga ccgctgtggg ctgaccgggt ttcccgccgc
     3841 gatggcgcgg ggctggttgg cgtgggtcct agctgagcgt ggagcgttcg acgaggggat
     3901 cgtctacgga caggacgggc tccgcctcgc cgaggcgctt gatcacccat acagctggat
     3961 cgccgcatct tgggggcccg cgcatctcta ccgcctgagg ggagaaatcg gccatgccgt
     4021 ccgcctcctc gagcgcgcac tctcgctctg ccgcgactgg gacatcctcg tgttgtcagg
     4081 gactaccgcg gggcacttgg ggttcgtgta cgtgcggtcg gggcgtgttg ccgagggcct
     4141 tgcgttgctg agcgaagggc aggagtccga gaagcagtac caaacgctca tcatcgcgca
     4201 catgggcgag gcatgcctgc tcgccgggcg ggtcgatgag gcgatcacat tcgccgaccg
     4261 cgccctcacc tttgcccgag agcgcggtca acgcggctac gaagcctatg ccctgcgcct
     4321 cctgggggag atcgcggcgc atcccgattc ccccgatgcc gaagcggccg aaggctactg
     4381 gcgccaggcc atggcgctcg ccgacgaact cggcatgcgc cccctcgtgg cccactgcca
     4441 cctcggcctc ggcaagctct accggcgcac gggcaagcgc gagcaggcac aggagcacct
     4501 caccaccgcg acgacgatgt accgcgagat ggggatgacg tactggctgg agcaggcgga
     4561 ggcggagatg aacgattgag atgaactctc accgccatta cctcgccctt gcccatatga
     4621 actccttcgc ctagcctgtc cccggccgag tcataggagg caaggggagg ctaatgggtc
     4681 ggggtccaaa gcccgccaag tccaaggaga tcaagcgccc tgttcgtcgc aagtcgccca
     4741 aagccaatgc cagagtgcgc gacctggaga aggccctagc ggaggcgcga gagcgtgagc
     4801 gagcgacggg cgaaatcctg
//
LOCUS       DASRJQ010000121         2478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1095506, whole genome shotgun sequence.
ACCESSION   DASRJQ010000121 DASRJQ010000000
VERSION     DASRJQ010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095506"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="VFB89_02825"
     CDS             <1..810
                     /locus_tag="VFB89_02825"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HZM26266.1"
                     /translation="ANPGVDPWLPGEGTNVVIPTRFVLPNAPRTGIVLNIPEMRLYYY
                     PPPTQGEKPIVMTFPVGIGREGWTTPYVVTKVTAKQRDPTWYPTESVRKEHAAEGDIL
                     PPAVPPGPDNPLGAYALRLAIPSYLIHGTHKPAGVGLRVSHGCVRMFPEDIETLFKLV
                     PIGTQVRVIDQPFKMGWQAGALYLEVHPPLDEDVKRTAKGLTAITELLVSTTADRQIE
                     VDWDAVEAIYDQATGIPQAMTRDQPMRTAEAGRGSDQRCPAGSKGGACRRD"
     gene            complement(910..1146)
                     /locus_tag="VFB89_02830"
     CDS             complement(910..1146)
                     /locus_tag="VFB89_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine-zipper protein"
                     /protein_id="HZM26267.1"
                     /translation="MKRDIEQAKQTAQQAANDAAAAKRTADGAAAAANEAKTMASNAQ
                     STADAAKRAADQANACCAANTEKIDRAFKTSNRK"
     gene            1940..2025
                     /locus_tag="VFB89_02835"
     tRNA            1940..2025
                     /locus_tag="VFB89_02835"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1974..1976,aa:Tyr,seq:gta)
     gene            2101..2174
                     /locus_tag="VFB89_02840"
     tRNA            2101..2174
                     /locus_tag="VFB89_02840"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2133..2135,aa:Gly,seq:tcc)
     gene            2199..2274
                     /locus_tag="VFB89_02845"
     tRNA            2199..2274
                     /locus_tag="VFB89_02845"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2232..2234,aa:Thr,seq:ggt)
     gene            2354..>2478
                     /locus_tag="VFB89_02850"
     CDS             2354..>2478
                     /locus_tag="VFB89_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZM26268.1"
                     /translation="MAKAKFERTKPHVNVGTIGHVDHGKTTLTSALTKVLAQKYGG"
BASE COUNT          431 a          806 c          817 g          424 t
ORIGIN      
        1 gcgaacccgg gcgtcgatcc ctggctccct ggcgaaggca ccaacgtcgt cattccgacc
       61 cgcttcgtgc tgccgaatgc gccgcggacc ggcatcgtac tcaacattcc cgagatgcgg
      121 ctctactact atccgccgcc cacgcaaggc gagaagccga tcgtgatgac ctttccggtc
      181 ggcatcgggc gcgagggatg gacgacgcca tacgtggtca cgaaggtgac cgcgaagcag
      241 cgcgacccga cctggtatcc cacggagtcc gtgcgcaagg agcacgctgc ggagggcgat
      301 atcctcccgc ccgcggtgcc gccgggtccg gacaatccgc tcggcgcgta cgctctccga
      361 ctcgccatcc cgagctacct gatccacggg acgcacaagc ccgcgggcgt gggcctgcgc
      421 gtgtcgcacg gctgcgtgcg catgttcccc gaggacatcg agacgctctt caagctcgtg
      481 ccgatcggga cgcaggtgcg cgtcattgat cagcccttca agatgggctg gcaggccggt
      541 gcgctctacc tcgaggtgca cccgccgctc gacgaggatg tcaagcggac ggcgaagggc
      601 ctgaccgcca tcacggagct gctcgtcagc acgaccgcag accggcagat cgaggtggac
      661 tgggacgccg ttgaggcgat ctacgaccag gcgaccggca tcccgcaggc gatgacacgc
      721 gaccagccca tgcgcaccgc ggaagcaggc cggggctcag atcagcgctg cccagccggt
      781 tccaaaggcg gagcctgtcg ccgcgactga gcgcgccacc ccgtcaggcg cgcttctcgg
      841 gcacaatgaa aacgccgcgc gagtgcgcgg cgccttcatt cagagctcgg aaagctctgt
      901 cctgcgggct tatttcctgt tcgacgtctt gaacgcccgg tcgatcttct cggtgttggc
      961 tgcgcagcag gcattcgcct gatcagcggc gcgcttcgcg gcgtcggcgg tcgattgcgc
     1021 attcgacgcc atcgtcttgg cttcgttcgc cgcggcagcc gcgccgtcag cagtacgctt
     1081 tgccgcagcc gcgtcgttgg ccgcctgctg ggccgtctgc ttcgcttgtt caatgtcccg
     1141 cttcattgct gtacagccgc tcatcaaggc gacggacagc gcgactgcta ccagcttggt
     1201 gcttgtcact cgcatgcgaa tctcccctgg cccttgttgc tctgcatgtg gaatccgcgg
     1261 tcctaacccc ggaagcccgc gcgtattttt agggaaagcg cagggctaag tcaaacatat
     1321 gcgcaattga ggcgctgtgg cgaattgcca acgccttgca aacggagcgg gtcggcggag
     1381 gctgaaaatc cggcatttct tcgcgtccga cgtgcgtgac gtgcgagctg cgcaagcatg
     1441 ccgggaacgc agtcgatacg accatggttg tatgacgcaa cctcgcgtcg agcccggcgc
     1501 gtagcatgga aggcgctcgc tgcccgagtc ctcgccggcc ccgacgctga gcccgcactc
     1561 gctcgcatcg gtctgcagag ctccgcgacc cgcagtctcc ggctggtgcc tcgccgagcc
     1621 gcgtcgggag gggccgggcg gtgagccaca cgcctcgcgt ctccagcggg gcggcctcaa
     1681 cggctcacgc agcccgttga cgcaccatgc tgaatgcatc tcaagccctt gagccgaaag
     1741 ctcgaatgcg gatgacgggg gctcggcgct cagtacggcg gtctcggccc gttccccgtc
     1801 tcgcggtgct tcgcgacgag gtcgcggagc cggtccgatc cgccgtcccg gggcgggcaa
     1861 gcaagcatcg ggccaggaag gcgttttggc gtattgacac tcccttccct ggacaggaca
     1921 atgcccggct cgtcgccgcg gaggggtact caagcggtca acgagggcag actgtaaatc
     1981 tgctggcttc ggcctacgta ggttcgagtc ctaccccctc caccaatgta cagccggtgg
     2041 gtgaacggag ggcgccgcga gtgcatgtgg ccgcgcgcga acgatcgccg ccgtcacaat
     2101 gcgggtgtag ttcaatggta gaacctcagc cttccaagct gatgacgtgg gttcgaatcc
     2161 catcacccgc tccaatcgga gcgcggcaag ttgtgagggc ccacatagct cagtcggtag
     2221 agcacttcct tggtaaggaa gaggtcaccg gttcaaatcc ggttgtgggc tccaggtcga
     2281 agcggctgaa ccctgagcgc cgggcgtgcc gaacgcaatt ggatatctga gaaatcgagg
     2341 ggggtagggt caagtggcga aagcgaagtt cgagcgtacg aagccgcacg tgaacgtcgg
     2401 gacgatcggg cacgtggacc atggcaagac gacgttgacg tcggcgctga cgaaggtgct
     2461 ggcgcagaag tacggcgg
//
LOCUS       DASRJQ010000122         2191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2764217, whole genome shotgun sequence.
ACCESSION   DASRJQ010000122 DASRJQ010000000
VERSION     DASRJQ010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2764217"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..985)
                     /locus_tag="VFB89_02855"
     CDS             complement(149..985)
                     /locus_tag="VFB89_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HZM26269.1"
                     /translation="MSATDGAIRADAFVCSYPKSGRTWMRFALAHYMRAVYGLELDLE
                     LRTMFRLLPNMDGYAPDLGKDASVYAFADRPEIPLVLSSHMSFDERLFGRRPVVFILR
                     CPYDVMVSQYFQKTRQDFSWQGPLKAFLRDCEVGVADLVRYLNSWGPCLQTSRALVLT
                     YEQLRRYPETGFLRLVAHLGIQTERAVARAALSEASFGRMREVERRFGIEGYDYDWTD
                     PEARRVRRGQIGGYRDYLDEQDCRYISTECARRLAPAARALLERWGLAPWGVPTRLPG
                     LR"
     gene            1120..2172
                     /locus_tag="VFB89_02860"
     CDS             1120..2172
                     /locus_tag="VFB89_02860"
                     /inference="COORDINATES: protein motif:HMM:NF020811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HZM26270.1"
                     /translation="MSADLARDCRSALADLPTEWATTPVKHKRPYRPHWSEEPPVERG
                     LVVEEIERGHATGFALRTGPVSRGIFALDEDGPGALALLAGKPGELPRTLLSASGRLG
                     HGQWFFTVPEEGWPAVRNRTVLTGQGEGVTPMKASLRFGSMISCLPPGLHPAGRRYRW
                     ETGLGPGEIEPAEIPRLLLDQAEYEPQESASIVNAAWRGDLARVAEALADGSAPDAPA
                     ADGYTALLRAVWAGHTACARLLLEAGADPDQRDPLGRPALLLASEVAHTGVMAALLEA
                     GAGVATRDPDGRTPLHGACWGGHAAAVEMLIAAGADHLTRDGDRREPGDEARAWGYDG
                     LVESHLARRRPVAFSR"
BASE COUNT          366 a          713 c          788 g          324 t
ORIGIN      
        1 gccacgggag cgcttcccgc tgattccgag atattggcga ggggttgagt agctgagcga
       61 gcagacggac cgagggaacg ccgcgcgtgg ctttcgagcc attggaggaa aagtcttcct
      121 gcggcgccgt ccggactgat cggaacgact accgcaagcc gggcaggcgc gtggggacgc
      181 cccagggcgc gagaccccat cgctcaagta gggcgcgagc cgcgggtgcg agtctccgcg
      241 cgcattcggt gctgatatag cggcagtcct gctcgtcgag atagtcgcgg taaccaccga
      301 tttgccccct acgcacgcgg cgcgcctcgg ggtcagtcca gtcgtagtcg tagccctcga
      361 tgccgaatcg acgctcgacc tcgcgcattc tcccgaacga ggcctccgag agggcagcgc
      421 gtgcgaccgc gcgctcggtc tgaatgccga gatgcgcgac caggcgcagg aaaccggtct
      481 cgggatagcg gcgcagctgc tcgtaggtca gcaccagggc gcgagatgtc tggagacacg
      541 gcccccacga attgaggtag cgcacgagat ccgcgacccc gacctcacaa tcgcgcagga
      601 aggccttcag tggtccctgc cagctgaagt cctgccgggt cttctggaag tactgcgaga
      661 ccatcacgtc gtaggggcag cgaaggatga agacaaccgg ccggcgtccg aagaggcgct
      721 cgtcgaagct catgtgactc gagagcacga gcgggatctc gggccgatcg gcgaaggcgt
      781 agaccgatgc gtccttacca aggtcaggcg cgtatccgtc catgttgggg agcaggcgga
      841 acatcgtccg cagctctaga tccagctcca ggccgtagac ggctcgcatg tagtgggcga
      901 gcgcgaaacg catccaggtg cgcccggact tcggatacga gcacacgaat gcgtctgcgc
      961 ggatggcgcc gtctgtggcc gacatggccc gggcagtcgg cgatccatac acgcgagcgc
     1021 ccactataag agggacacgg gcgccaatcg ctcggccgaa cggccgggcg atccggcccg
     1081 cctgtcgccc ggatacggtt tgccaacgtg agaggagcct tgagtgcaga cctggcccgc
     1141 gattgccgct cggcgctcgc ggatttgcca acggagtggg ccactacccc ggtcaagcac
     1201 aagcggccct accgaccgca ctggagcgag gagccgcccg tcgagcgagg gctggtggtc
     1261 gaggagatcg agcgcggcca tgcgaccggc ttcgccctgc ggaccgggcc cgtctcacga
     1321 gggatcttcg ccctcgacga ggacggtcca ggcgcgctgg cccttctggc gggaaagccc
     1381 ggcgagctgc ctcggacctt gttgagcgcg agcgggcggc tgggacacgg gcaatggttc
     1441 ttcacagtgc cggaggaagg ctggccggcg gtgcgcaacc gcaccgtatt aaccggccag
     1501 ggcgagggcg tgacccccat gaaggcctcg ctgcgcttcg gttcgatgat ctcgtgcctt
     1561 ccgccaggac tgcaccctgc cggtcgccgc taccgctggg aaaccggact ggggccgggc
     1621 gagatcgaac cggcagagat cccccgattg ctgctcgacc aagccgagta tgagccgcag
     1681 gagtcggcgt cgatcgtcaa cgcggcgtgg cgaggggacc tggcgcgcgt ggcggaagcg
     1741 ctcgccgacg gcagtgctcc ggacgcgccc gccgccgatg gctacaccgc gctcctgcgc
     1801 gccgtctggg ccggacatac ggcctgtgcg cggctgctgc tggaggcggg cgctgacccc
     1861 gaccagcgtg acccgctggg gaggccggcg ctgctgctcg catcggaggt ggcccacacc
     1921 ggtgtcatgg ccgcgctctt agaggctggg gccggcgtcg cgacccgcga tcccgatggc
     1981 cgcacgccgc ttcatggcgc gtgctggggc gggcatgcag cggcggttga gatgctcata
     2041 gccgccggcg cagaccatct gacccgggat ggtgatcggc gcgagccggg cgacgaggct
     2101 cgcgcgtggg gatacgacgg gcttgtcgag tctcatctcg cccggcgcag gcctgtcgcg
     2161 ttctccagat agcccagttc ggcaagcatg g
//
LOCUS       DASRJQ010000123         2392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1884284, whole genome shotgun sequence.
ACCESSION   DASRJQ010000123 DASRJQ010000000
VERSION     DASRJQ010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1884284"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VFB89_02865"
     CDS             <1..240
                     /locus_tag="VFB89_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26271.1"
                     /translation="FLVHAAALEMHPLDTADRTAELRTAHGIGYCNITKCCTKVCPEN
                     ITITDNAIIPLKERVADRAYDPLRALLRMVGLVDR"
     gene            250..786
                     /locus_tag="VFB89_02870"
     CDS             250..786
                     /locus_tag="VFB89_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26272.1"
                     /translation="MLSMLNWETRTAEMVKQLQDRVATGTAAVILVRWIVDEAEDFRL
                     VHVRERDPVAHLRALVEAGGGPIGLLEWTGDGQNKLSVAFHPYEGYEDVTPRILMYMS
                     PPLRHAARLVCGSCGQATVHLVEDQARSPSACLHLILSVVTLGLWLLVLGFIVSRYER
                     AGCTLCGTVRRIRREGGS"
     gene            832..1332
                     /locus_tag="VFB89_02875"
     CDS             832..1332
                     /locus_tag="VFB89_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26273.1"
                     /translation="MFTPKRITRDGIDRALEKAEHYRLLNEPSDAESICQDILEIDPD
                     NQTALVALLLSRTDQFQSTHGASVAAAREVLPRLTSDYQRHYYAGIICERWAKARHAH
                     GAPGAGATVYHWLREAMDCYERAEAIRPAGNDDALLRWNACARLLARHPELQPGVEDR
                     EEVMLE"
     gene            1437..1943
                     /locus_tag="VFB89_02880"
     CDS             1437..1943
                     /locus_tag="VFB89_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26274.1"
                     /translation="MEFFKRATREGFAAGLIGAGGVALWFLIVDTINGRPFYTPALLG
                     SAVFWGLRDPTQVHVAYSTIIGYSMLHVVAFWVVGSVAAALAALVDRVPTTLFLVVVF
                     FAAFEVGFYIIVAILAQPLLGALAWINVAIGNALAAAGMGYYLWHAHPHIREALAAHP
                     LGESEDWY"
     gene            complement(1940..>2392)
                     /locus_tag="VFB89_02885"
     CDS             complement(1940..>2392)
                     /locus_tag="VFB89_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HmuY family protein"
                     /protein_id="HZM26275.1"
                     /translation="LATHTVPTYVPRSRMPATASRPMVDTVTIDARDPERWQFIAFDT
                     GGWDIAVRRFHVMVAGDAIGIDSVPFEALRSAPSVGYVATTFERDTVNEAIARWYRYS
                     MFSHLLRPKKRIYVIRSRSGRATKLEFLSYYCPGPEPACVTFRFETLR"
BASE COUNT          411 a          738 c          791 g          452 t
ORIGIN      
        1 tttctggtgc acgccgcggc cctggagatg cacccgctcg atacggcgga ccgcaccgcc
       61 gagctgcgga ccgcgcatgg catcggctac tgcaacatca ccaagtgctg taccaaagtg
      121 tgtcccgaga acatcaccat caccgacaac gcgatcatcc ctctgaagga gcgggttgcc
      181 gaccgcgcct acgatccgtt gcgggcattg ctccgaatgg tgggtctggt cgatcgctga
      241 tgcgaaaggg tgctgtccat gttgaactgg gaaacgcgga cggcagagat ggtcaagcaa
      301 cttcaggacc gggtggctac cggtaccgcc gccgtgattc tcgtacgatg gatcgtggat
      361 gaggcagaag acttccggct cgtgcacgtg cgggaacgtg acccggtggc tcatctacgg
      421 gcccttgtcg aggctggggg gggcccgatt ggcctgctcg agtggacagg ggacggccag
      481 aacaagctca gcgtcgcatt tcatccatac gaaggctacg aagacgtgac gccgcgcatt
      541 ctgatgtaca tgtctcctcc gcttcgtcat gccgcgcgtt tggtctgcgg ctcgtgtggc
      601 caggcgacgg tgcacctcgt ggaagaccag gcgcgttctc cttcggcctg ccttcacctg
      661 atactctccg tcgtcactct cggactctgg ttgctggtat tgggttttat cgtttcacgc
      721 tatgagagag ccggctgcac gctgtgtggg acggtccgca ggattcgacg cgagggtgga
      781 tcgtgattcg ccgcgaccgg attatcgtgg aatcgtgctg aggagatgcc catgttcaca
      841 ccgaagcgaa ttacgcggga tggcatcgat cgggcgctcg agaaggccga gcactaccgg
      901 ctgttgaacg agccgagcga tgccgaaagt atctgccagg acattctcga aatcgatccc
      961 gacaatcaga ccgcgttggt ggccctgctg ttatcccgta ccgatcagtt tcagtcgacg
     1021 cacggagcca gtgtcgcggc ggcgcgggag gtgctgccgc ggctcacgag tgactaccag
     1081 cgccactatt acgccgggat catctgcgag cggtgggcca aggcgcggca cgcccacggc
     1141 gcccccggtg ccggtgccac cgtgtaccac tggctccgtg aagcgatgga ctgctacgaa
     1201 cgggccgaag ccatccggcc ggcgggcaac gacgacgcgt tgctgcgatg gaacgcctgc
     1261 gctcgtctcc tggcgcgcca tccggagctg cagccggggg tcgaggatcg ggaggaagtg
     1321 atgctggagt aggggggctg ggcactccaa agagccaaaa cccgcgcatc gtctcccctc
     1381 gcgaggtctg tccgtaaccg tatattgggc tcccgaagcc atctacggga gctgccatgg
     1441 agttcttcaa gcgtgcgacg cgagaaggct tcgccgccgg gctcatcggt gccggcggag
     1501 tggctctctg gttcttgatc gtcgacacga ttaacgggcg gccgttttac acgcctgcct
     1561 tgctcgggtc ggcagtgttc tgggggctgc gcgacccaac ccaggtacac gttgcctatt
     1621 ccacgatcat cggctacagc atgctccacg tagtcgcgtt ctgggtggtc gggtcggtgg
     1681 ccgccgcact cgcggcgctg gtcgaccgcg tgcccacgac gctcttcctg gtggtggtgt
     1741 tcttcgcggc cttcgaggtc ggcttctaca tcatcgtggc catactggcc cagccccttc
     1801 tcggcgccct ggcatggatc aacgtcgcga tcgggaatgc cctcgcggcg gccggcatgg
     1861 gctactatct ctggcacgcc catccgcaca ttcgcgaggc gctcgccgcc cacccgttgg
     1921 gagaatccga ggactggtac tagcggagcg tctcaaagcg gaatgtgaca caggccggct
     1981 ccggccccgg acagtagtag ctcaagaact ccagcttggt ggcccgtccg gaacgcgagc
     2041 ggatgacgta gatccgtttt ttcgggcgca acaggtggga gaacatactg taccggtacc
     2101 accgcgcgat ggcttcattc accgtgtcgc gctcgaaggt ggtggcgacg tatccgacgg
     2161 acggggccga gcgcagcgcc tcgaagggga ccgagtcgat cccgatcgcg tctcccgcca
     2221 ccatcacatg aaagcgccgc acggcgatgt cccatccccc ggtgtcgaag gcgatgaact
     2281 gccaccgctc gggatcgcgc gcgtcgatcg tgaccgtgtc gaccatcggc cgtgatgccg
     2341 tcgccggcat gcgggatcgg ggtacgtaag tcgggacggt atgggtggcg ag
//
LOCUS       DASRJQ010000124         3218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2452711, whole genome shotgun sequence.
ACCESSION   DASRJQ010000124 DASRJQ010000000
VERSION     DASRJQ010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2452711"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="VFB89_02890"
     CDS             <1..1341
                     /locus_tag="VFB89_02890"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZM26276.1"
                     /translation="AGIVSALAGLCYAELASTVPMAGSAYTYAYATLGEFVAWIIGWD
                     LIVEYAVGNIGVAIGWSGYFRELISHFGVRMPAWLATDYRSAHDAAAAVAAGATDATH
                     TYLASAVAEAPKLFGIPFIINLPAFLVVAAITVILVRGVRESANSNNAMVLLKIGIIL
                     FFVAVGVFLIKPENWTNPATGGFAPNGFAGIGAGAAIIFFSYIGFDAVSTAAEESKDP
                     KRDMPFGIIMSLVVCTVLYIALSAVMTGMAPWKNLGTAEPMITALQYSDGPPALINAS
                     RFIVSLGAVIAMGSVLLVFQLGQPRIFFSMARDGLLPPFIAKVHPRYRTPWVGTIITG
                     MFVGGFAAFANIAEVVDLTNIGTLFAFVLVSLGVIALRYTDPQRGRPFRAPGVPITPL
                     ISVAACLYLMLQLPAITWKRFAIWLVLGLVLYFAYGSRHSRLRNPKPAAMSDAA"
     gene            complement(1364..2314)
                     /locus_tag="VFB89_02895"
     CDS             complement(1364..2314)
                     /locus_tag="VFB89_02895"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HZM26277.1"
                     /translation="MRMDSRERDYDLPSPSMSEVLVGADVGGSKTAVAVEKAGTIAAR
                     ATGPGAAVRPGRALQSAATISEVVRRALAGAAELRASVLVVGAAGAGRQPEQGELERA
                     LRGEQIAEKVVVVTDIEIALVAAFEDAPGIVVSAGTGSIAVGRDRQGKFKRMGGYGWQ
                     MGDEGSGYAIGRAALGAVSRARDGRSPSTPLQHRILAATRSSNFDELVSWAATASPAE
                     VAALAPHVLEVAGAGDVVARGILDYAARELSQLAICLQPQLGERDKISVALAGGLLEA
                     STPLRGVVLAKLAEHDHFTVVDTPIDAALGALAIAKRPAP"
     gene            complement(2295..>3218)
                     /locus_tag="VFB89_02900"
     CDS             complement(2295..>3218)
                     /locus_tag="VFB89_02900"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26278.1"
                     /translation="AFAAVIALHFFLQGGWTGALIAAGQAGKLRVLDFQPTTSVVYTF
                     WAGLVGGALFSMASHGTDHLIVQRLLATRSLRDARVALVGSGVVVFLQFLLFLLVGSA
                     IWATKLMPDTIASDQIFPRFVISNLPAGLAGLVVAGILAAAMSTHSSAINALASSLTH
                     DLYAGWTGRKDPAHLLRVGRIFSAVWAVALMLGALLFLAYDREGKTPVVVLALSVASI
                     AYGGLLGAYLLAGRWARATSRGVIIGVTVSLAIMLVVFFARGLSDRFGIAWLAAVGRL
                     AWPWYVPLGTLITVTVGLVASVGGNHADGQS"
BASE COUNT          541 a         1118 c         1009 g          550 t
ORIGIN      
        1 gccggcatcg tcagcgcgct ggccgggctc tgctacgcgg agctcgcgtc gaccgtgccg
       61 atggccgggt cggcgtacac ctacgcgtac gcgacgctcg gggagttcgt ggcctggatc
      121 atcgggtggg acttgatcgt cgagtacgcg gtcgggaaca tcggcgtggc tatcggctgg
      181 tcgggctact ttcgcgagct gatcagtcat ttcggcgtcc gaatgccggc ctggctcgca
      241 accgactacc ggagcgccca tgatgcggcc gctgcagtcg cggctggcgc caccgacgcg
      301 acccacacgt atctcgccag cgcggtcgcc gaagcgccca agctgttcgg catccccttc
      361 atcatcaatc tcccggcgtt cctcgttgtc gccgcaatca ctgttattct ggttcgcggc
      421 gtgcgcgagt cggccaactc gaacaacgcg atggtgctgc tcaagatcgg gattattctg
      481 ttcttcgtcg cagttggcgt gttcctgatc aagccggaga actggaccaa tcccgcgacc
      541 ggcggcttcg ctcccaacgg gttcgccggg attggcgccg gggccgccat catctttttc
      601 agctatatcg gcttcgacgc ggtctccacc gcggcggagg agtcaaaaga tcccaagcgg
      661 gatatgccgt tcgggatcat catgagcttg gttgtttgca ccgtcctcta cattgcgctc
      721 tcagcggtga tgaccgggat ggcgccctgg aagaacctcg ggaccgcgga gccaatgatc
      781 acggcccttc agtattcgga tggaccgcca gcgctgatca atgcctcgcg attcatcgtc
      841 tcgctgggtg cggtcattgc gatgggaagc gtcctgctcg tctttcagct gggccagccc
      901 cgcatcttct tctcgatggc tcgtgatggc ctgctgccgc cctttatcgc caaggtgcat
      961 ccgcgctacc gaacgccgtg ggtcggcacc atcatcaccg gtatgttcgt cgggggcttc
     1021 gccgcgttcg ccaacatcgc cgaagtcgta gacctcacca acatcggcac cctgttcgcc
     1081 ttcgtcctgg tatccctcgg cgtcatcgcc ctccgctaca ccgatccgca acggggtcgc
     1141 ccgttccggg ctccgggggt accgatcacg ccgctcatct cagtcgcggc ctgcctgtac
     1201 ctcatgttgc agctccccgc catcacctgg aagcgcttcg cgatctggct ggtcttgggg
     1261 ttggtgctgt acttcgcgta tggatcgagg cacagccggc tgaggaaccc caagccggca
     1321 gcgatgtccg acgctgccta gctgactgat cgaccggaac ggcctaggga gcgggccgtt
     1381 tggcgatggc cagtgcgccc agcgcggcat cgatcggcgt gtctacgacg gtgaagtggt
     1441 cgtgctcggc gagcttggcc agcaccacac cgcgcagcgg agtactggcc tcgagcagtc
     1501 cccccgccag cgccacggaa atcttgtccc gttctcccag ctgtggctgc aagcagatcg
     1561 cgagctgcga cagctcccgc gccgcatagt ccagaatccc gcgggccacc acgtcaccgg
     1621 ctcccgccac ctcgagcacg tggggcgcca gagcggcgac ctcggccgga ctcgccgtcg
     1681 cggcccaact caccagctcg tcgaagttgc tgctgcgagt tgcggcgagt atccggtgct
     1741 gcagcggtgt gctcggactg cgcccgtcgc gcgcgcggct cactgcaccc agggctgcgc
     1801 ggccgatggc gtatccgctc ccctcgtcgc ccatctgcca accgtagcca cccatccgct
     1861 tgaactttcc ctgccggtct cgtccgactg cgatactccc ggtaccggca ctcaccacga
     1921 tgcccggtgc atcttcgaac gccgcgacca gggcgatttc gatgtcggtg acgaccacga
     1981 ctttctcggc gatctgctcg ccccgcagcg cccgctcgag ctcaccctgc tcgggctgtc
     2041 gccccgcgcc cgcggcgccc acgacgagga ccgaggctcg cagttcggca gcgcctgcaa
     2101 gggcccggcg caccacctcg gaaattgtgg ccgccgactg aagggcccgg ccggggcgaa
     2161 ccgcggcgcc aggcccggtg gctcgggccg ctatggtgcc tgccttctcg accgcaaccg
     2221 cggtcttgct tccgccgaca tcggcgccga ccagcacttc gctcatagat ggggaaggaa
     2281 gatcgtagtc tcgctcacga ctgtccatcc gcatgattcc caccgacgct cgcgaccagg
     2341 ccgactgtga cggtaatgag cgtacccaag ggcacgtacc atggccatgc caaccgtcca
     2401 acggctgcta gccaggcaat gccgaaccgg tctgacagac cccgggcgaa aaagacaacc
     2461 agcatgatcg caagcgacac cgtcacgccg atgatgacac cgcggctcgt ggcgcgagcc
     2521 cagcgtccgg cgagcaggta cgcgcccagc agcccgccgt atgcgatgga tgcgaccgac
     2581 agggccagca ccacgacagg cgtcttgcct tcgcgatcgt aggctagaaa cagaagtgct
     2641 cccagcatga gcgcgacggc ccaaactgct gagaagatgc gaccgacgcg aagcagatgg
     2701 gccggatcct tccttccggt ccagccggcg tacaggtcgt gcgtgaggga cgaggccaac
     2761 gcgttgatcg ctgaactgtg cgtgctcatg gcggccgcga ggatgccggc caccaccagc
     2821 cctgccagcc cggccgggag attgctgatg acgaaacggg gaaagatctg atccgatgcg
     2881 atcgtgtcgg gcatcagttt ggtcgcccag atggcggacc cgaccagaag gaacaggagg
     2941 aattggagaa agaccacgac ccccgacccg accagcgcga ccctcgcgtc gcgcagcgag
     3001 cgagtggcca gcaaccgctg cacgatcagg tgatcggtgc cgtgcgaggc catcgagaag
     3061 agcgcaccgc cgaccagccc cgcccagaac gtgtacacca ccgacgtcgt cggctggaag
     3121 tcgagcacgc ggagcttgcc cgcctgcccc gcggcgatga gcgcgcccgt ccatccgccc
     3181 tgcaggaaga agtggagcgc gatcacggcg gcgaacgc
//
LOCUS       DASRJQ010000125         3046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1320183, whole genome shotgun sequence.
ACCESSION   DASRJQ010000125 DASRJQ010000000
VERSION     DASRJQ010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1320183"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VFB89_02905"
     CDS             <1..216
                     /locus_tag="VFB89_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL13 family ribosomal protein"
                     /protein_id="HZM26279.1"
                     /translation="FRHTGYMGHERFTPLATMLAKHPDRVIERAVWGMLPKTTLSRQK
                     VKQMLKVYAGPNHPHAAQQPQPLTVTP"
     gene            213..614
                     /gene="rpsI"
                     /locus_tag="VFB89_02910"
     CDS             213..614
                     /gene="rpsI"
                     /locus_tag="VFB89_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HZM26280.1"
                     /translation="MTETPELRWHAIGRRKESVARVYLTPGSGKWNVNGRTLGDFFPR
                     ASLVSHIQQPFTATDTLGAFDVKGICRGGGVTGQAGAMRLGIARALCLADERHRKKLR
                     EQGLLTRDPRAVERKKPGRAGARKRFQFSKR"
     gene            782..1672
                     /gene="rpsB"
                     /locus_tag="VFB89_02915"
     CDS             782..1672
                     /gene="rpsB"
                     /locus_tag="VFB89_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HZM26281.1"
                     /translation="MTQPQVQDLLEAGVHFGHQTRRWNPKMRRFIFAERSGIYIIDLQ
                     KTLRQLEQAQELLRGIVLAGNGVLFVCTKKQLKLIIQGEAQRAGGFWVTERWLGGTLT
                     NFQTIKKQIKRLKELEQGAAEGEFEHYTKKEQLLLDRERVKLDKNLSGIKQMGRLPGA
                     LFVVDAKKERIAVAEANKLGIPVVAIVDTNADPDIITVPIPGNDDAIRAVSLIATAVA
                     EVISEARRQQPLRDAVEDAEAQTYSTETGVETESEGDRKKRTAGRRKRRPKPEAIAAR
                     LKSDDAATAVEPAAGGGGES"
     gene            1780..2379
                     /gene="tsf"
                     /locus_tag="VFB89_02920"
     CDS             1780..2379
                     /gene="tsf"
                     /locus_tag="VFB89_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HZM26282.1"
                     /translation="MKEIPAKLVAELRAKTGAGMMDCKKALEESGGDIAKAEEILRVK
                     YAGKAEKRAAKSASEGLIDAYVHFNGKVAVLVELNCETDFVARTDDFRQLAKDVALQI
                     ASAKPVAVRIEDLPAEVVERERRIYEAQVVEQKKPEPIRAKIVEGMLKKFYEENVLLE
                     QKFIKDDKQTVGDLVRGIAGKTGEKVEVRRFARFELGGA"
     gene            2380..>3046
                     /gene="pyrH"
                     /locus_tag="VFB89_02925"
     CDS             2380..>3046
                     /gene="pyrH"
                     /locus_tag="VFB89_02925"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HZM26283.1"
                     /translation="MALVYPRALLKVSGEALAGGADSGIDFGVVDRLTNELKQVAASG
                     VQLGLVVGGGNIVRGAQASGQGMDRVSADYMGMLATVINALALQDILEKKGLDTRVMS
                     AIRMEELAEPYIRRRAVRHLEKGRVVIFAGGTGNPYFSTDTAAVLRALEIEAQVILKA
                     TNVDGIYTADPKKDPKATFLAELTFQEALVNGYAVMDANAFGLCKENRLPIVVFNINQ
                     PGAT"
BASE COUNT          606 a          871 c         1106 g          463 t
ORIGIN      
        1 ttccggcaca ccgggtacat gggacatgag cggttcacgc cgctcgccac gatgctcgcc
       61 aaacatcccg accgcgtgat cgagcgggcc gtatggggga tgttgcccaa gaccacgttg
      121 tcgcgacaga aggtgaaaca gatgctcaag gtgtacgccg gaccgaatca tccgcacgcg
      181 gcccagcagc cgcagccgct cacggtaacc ccatgaccga gacgccggag ctgcgctggc
      241 acgcgatcgg acgccgcaag gagagcgtgg cgcgcgtcta tctgacgccc gggtcgggga
      301 agtggaacgt gaatggccgc accctgggcg attttttccc gcgcgcgtcg ctggtgtcgc
      361 acattcagca gccgttcacg gcgaccgaca cgctgggggc gttcgacgtc aagggtatct
      421 gccgtggcgg cggggtgacg ggacaggccg gcgcgatgcg gcttggcatc gcccgggcgt
      481 tgtgcctggc cgacgagcgg catcgcaaga agttgcggga gcagggactg ctgacgcgtg
      541 atccgcgggc cgtggaacgg aagaagccgg gccgggccgg ggcgcggaag cggttccagt
      601 tcagcaaacg gtaaacggtc atcggtcgtc ggtagctacc gaagaccgac aaccgattac
      661 cgaggacgca gttcacaaat actcacgtcg cccgatctgt gagccgggtt cccgcggtca
      721 gccgcagggt cgctccggag atgacggcgg cggcggtcga aaaacccagg aaagggagtg
      781 catgacgcag cctcaggtgc aggatctgtt ggaagcaggg gtgcatttcg ggcaccagac
      841 gcgacgctgg aacccgaaaa tgcgccgctt cattttcgcg gaacgctccg gcatctacat
      901 catcgacctc caaaagacgc tgcggcagct ggagcaggcg caagagctgt tgcgcggcat
      961 cgtgctggcc gggaacggcg tgctgttcgt ttgtaccaag aagcagctca agctgatcat
     1021 ccaaggcgag gcgcagcgag ccggtggttt ctgggtcacc gagcggtggc ttggcggcac
     1081 cctcaccaac ttccagacga tcaagaagca gatcaaacgg ctgaaggagc tggagcaggg
     1141 ggcggccgaa ggggagttcg agcattacac gaagaaggag cagctgctgc tcgatcgcga
     1201 gcgcgtcaag ctcgacaaga acctgtcggg gatcaagcag atggggcggc ttccgggcgc
     1261 cctgttcgtg gtggacgcca agaaagagcg catcgccgtc gccgaagcga acaagctcgg
     1321 catcccggtg gtggcgattg tggatacgaa tgccgatccc gacatcatca cggttccgat
     1381 tcccgggaac gatgatgcga tccgggcggt gtcgttgatc gcgacggccg tagctgaagt
     1441 catctccgag gcccgccggc agcagccgct gcgcgacgcg gtggaggatg cggaagccca
     1501 gacctactcg accgagaccg gggtcgagac ggaatccgaa ggcgatcgca agaagcggac
     1561 ggcggggcgg cgcaagcgga ggcccaaacc cgaagcgatt gcggcccggt tgaagagtga
     1621 tgatgccgcg acggcggtgg agccggcggc tggggggggg ggcgagtcct aagcggtcgg
     1681 tgagcgggac tgccaggggg ttcgaaccat gcgccgccca tcgcgtttca gcatcgcgag
     1741 ggcggcgttc tataggagga gtaacgacag gtgactacgg tgaaagagat tccggcgaaa
     1801 ctggtggcgg agctccgggc caagaccggg gccggcatga tggattgcaa gaaggcgctc
     1861 gaggagtccg gcggcgacat tgccaaggcc gaagagatcc tgcgcgtgaa gtacgccggc
     1921 aaggccgaga agcgcgccgc caagtcggcg agcgaagggt tgatcgatgc ctacgtgcac
     1981 ttcaacggca aggtggccgt gctggtggag ctgaactgcg agacggattt cgtcgcgcgg
     2041 acggacgatt tccgccagct cgccaaggac gtcgcgctgc agatcgcctc ggcgaaaccg
     2101 gtggcggtgc gcatcgagga cctcccggcc gaggtggtcg agcgtgagcg gcgcatctac
     2161 gaagcgcagg tcgtcgagca gaagaagcct gagccgatcc gggccaagat cgtcgagggg
     2221 atgctcaaga aattctacga agagaacgtg ctgctggagc agaagttcat caaggacgac
     2281 aagcagaccg tcggcgatct ggtgcgcggc atcgccggca agaccggtga aaaggtcgag
     2341 gtgcgtcgct tcgcccgctt cgagctcggg ggcgcctgag tggcgctggt gtacccgcgc
     2401 gcgctgctca aggtctccgg tgaggcgctt gccggaggag ccgattcagg gatcgatttc
     2461 ggggtcgtcg accggctcac gaacgagctg aaacaggttg cggccagcgg ggtgcagctc
     2521 gggctggtgg tgggtggggg caacatcgtt cgtggggcgc aggcgtccgg tcaggggatg
     2581 gatcgcgtct ccgcggacta tatggggatg ctggcgaccg tcatcaacgc cctggcgctg
     2641 caggacatcc tggagaagaa gggactcgat acccgggtca tgtcggcgat ccggatggag
     2701 gagttggccg agccctatat ccggcgccgg gcggtccggc atctggagaa ggggcgcgtc
     2761 gtcatcttcg cgggtggtac cgggaacccc tacttttcca ccgacacggc ggccgtgttg
     2821 cgcgccctgg aaatcgaggc ccaggtcatc ctcaaagcga ctaatgtgga cgggatctac
     2881 acggccgatc cgaagaagga cccgaaggcg accttcctcg ccgagctgac gttccaggaa
     2941 gcgctggtca acgggtatgc ggtaatggac gccaacgcct tcggcctctg caaggaaaac
     3001 cggttgccga tcgtcgtgtt caacatcaac cagcccggcg cgacgg
//
LOCUS       DASRJQ010000126         4677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_4142224, whole genome shotgun sequence.
ACCESSION   DASRJQ010000126 DASRJQ010000000
VERSION     DASRJQ010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4142224"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            485..1117
                     /locus_tag="VFB89_02930"
     CDS             485..1117
                     /locus_tag="VFB89_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HZM26284.1"
                     /translation="MTSKDRAPDFAIGRADGRIISLSHFAGYPLVLWFGGTRTDAFRS
                     DQLAWCTQLMAGVSDGDGELLAVEVDGAWCELKFRHQTVRVGLAAGLPADGPVARAYG
                     VEGGEAIFVIDGDGTIQWRHISPPDARPPIAAMLAALTRTAAEPGPRPSNRRDFVVAA
                     LAATLLLAAHPTMARARALSSITPRPGIEQATRSPSNTDPRSGQRDSEQK"
     gene            complement(1088..2407)
                     /locus_tag="VFB89_02935"
     CDS             complement(1088..2407)
                     /locus_tag="VFB89_02935"
                     /inference="COORDINATES: protein motif:HMM:NF013056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HZM26285.1"
                     /translation="MSVSRFFQLPECGTTIRTEVVAGATTFAALSYIIFVQPAVLSGA
                     GMDFGAVMAATCIASAFATGLMGLVANYPIAVAPAMGHNFFFVFAVTQLGVPWQVALG
                     ADFLAGALFVLLAAFRFREAIVDAIPACLKAAIPVGIGLLIAFVGLRWGGIIVANPGS
                     LVRLGRLTEPAPLLALGGFLLISVLELRRVPGAILIGIIATTLLGLVSGVRSYAGIVG
                     RPPSLGPTLLQLDIGSALSFRYLEIVLVFFFLAVFDTVGTLIGVGTQAGLVRDGKLPR
                     VGRALLADATGTVTGTLLGTSTVTAYVESAAGVAAGGRTGLANLVTAVLLLASLFLYP
                     LARLVGGGLPGPDGGMLYPVTAAALISVGALMLRNVTAVRWEDPADAIPAFLTIAIMP
                     LSLSIGDGLAFGFISFALLRALTGRAREVGWVVYLLAGLFLLRIALT"
     gene            2558..3349
                     /locus_tag="VFB89_02940"
     CDS             2558..3349
                     /locus_tag="VFB89_02940"
                     /inference="COORDINATES: protein motif:HMM:NF014609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HZM26286.1"
                     /translation="MPTPIVTVDAFTDRAFAGNPAAVCILDAPRSDDWMQLVAREMNL
                     AETAFLVRCGEEFGLRWFTPSIEVDLCGHATLASAHVLWEQGHLAQSDEARFHTRSGL
                     LKATQGDGWITLDFPATPARPIDPPPELARALAVPIRFYGKTPFDYLVEVESESAVRD
                     LAPDMALLAQLKGRGVIVTSRSTTPGQDFVSRFFGPAVGIPEDPVTGSAHCALGPLWG
                     ERLKKSSLVGFQASPRGGLVKVTLAGDRVLLGGQAVTVLRGELLA"
     gene            3346..3690
                     /locus_tag="VFB89_02945"
     CDS             3346..3690
                     /locus_tag="VFB89_02945"
                     /inference="COORDINATES: protein motif:HMM:NF007495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HZM26287.1"
                     /translation="MTAPTIDGLQAFQALDLRVGRVTRVEPNAKARKPAYQLWIDFGP
                     LGERRSSAQLTDGYRAEELVGRLVVAAVNLGTRMIAGVPSEVLVLGLPNDAGQVVLLA
                     VEREVPLGGRVY"
     gene            complement(3747..4190)
                     /locus_tag="VFB89_02950"
     CDS             complement(3747..4190)
                     /locus_tag="VFB89_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26288.1"
                     /translation="MTMPRLILRVAPLGLALLACAPGDRVAGKTLERGRIYTSWLYGA
                     EYGKLWARFTPEFQRVFGSAAELGAFAGRAVTQLGREQRTVDEHVAELKPEHVYSRTA
                     AFTAARQPVVIEWTLTKDGAVSGLAVRPAEPDSATATEELEQRER"
     gene            4189..>4677
                     /locus_tag="VFB89_02955"
     CDS             4189..>4677
                     /locus_tag="VFB89_02955"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HZM26289.1"
                     /translation="MRVYISVDMEGIAGVVHEDQTNPVDPRCAAEYARFRKLMTGEAN
                     AAVEGALAGGASQVVVNDSHWEMRNLLPEDLHEAAELLSGGPKRLSMLEGIEADFDAA
                     VFIGYHAKAGTAGAILDHTMMDAIRDVRLNGRSMGELGLNAALAGAHGVPVALVSGDR
                     ATA"
BASE COUNT          870 a         1518 c         1515 g          774 t
ORIGIN      
        1 acggcactcg ccgcaccgtg tgccaccggc gcggttcgcg cttcggcctt ttcgggcaaa
       61 tgagctcgtt ccggctccag gccgagtaaa aaggctgcgc ctccgacacc tgccagaaag
      121 cgaaccgcaa ccgcacgtcc gctcaaacgc cttttcggct cgccctgcac tggcgcggca
      181 tttctgtccc gtcgtttcat ggcgaaccgc cccctctctg ttcactgctg actacacaag
      241 cagcgtaccg aaaccagagg gaatcccccc gtgtcaaccc gcacacggac gccgtgtggc
      301 tgcgctcact tatgacttag gggtaggcgc ggaattgcgc gtagtggcgt gccttgcgcg
      361 tctcggcgtg acatgcgtcc aatgtgccgt caggtcgtga cggttctcac ggaaagagcg
      421 ccgccgaatg ccttatctgg cgcagtcaaa aatggactct tcaaaaacct gatcgaggcc
      481 gcgaatgaca tcgaaagacc gtgcaccgga cttcgcaatc ggccgcgccg atggacggat
      541 catcagcctg agtcatttcg cgggctatcc tctcgttctc tggttcggtg gcacccgcac
      601 cgatgccttt cggagcgatc agttggcatg gtgcacccag ctcatggccg gggtatcgga
      661 tggagacggc gaactcctgg cagtcgaagt cgacggtgct tggtgtgagc tcaagttccg
      721 gcaccagacg gtgcgagtgg gcctggccgc gggcctgccc gcagacggcc cagtggcccg
      781 agcgtacggg gtcgagggcg gcgaagcgat cttcgtgatc gacggcgacg gcacgatcca
      841 atggcgacac atttcgccgc ccgacgcgcg gcctcccatc gctgcgatgc tggcggccct
      901 gacccggaca gctgctgagc ccggcccccg tccgtccaat cggcgagact tcgtcgtcgc
      961 ggcacttgcg gcgacgctcc tcctcgccgc tcatcccacg atggcaagag cgcgggcgct
     1021 ttcgagcata accccgagac ccgggatcga gcaggctacg cgctcgccct cgaatacaga
     1081 ccccaggtca ggtcagcgcg attctgagca gaaatagccc tgccagcagg tagaccaccc
     1141 accctacctc gcgagctcgc ccggtcagcg ctcgcagcaa cgcgaagctg atgaagccga
     1201 acgcgagtcc gtcaccgatg ctgagcgaga gcggcatgat ggcaatggtg agaaatgcgg
     1261 gaatcgcatc ggccggatct tcccaacgga ccgccgtgac gttgcgcagc atgagtgcgc
     1321 cgaccgagat gagcgcggcc gcggtgacgg gatagagcat cccgccgtcc gggccgggaa
     1381 gtccgcctcc gacgaggcgg gccagcgggt agagaaaaag cgaggcgagg agaagcacag
     1441 ccgtgaccag attcgcgagg ccggttcggc cgccggccgc gacccccgcc gcgctctcga
     1501 cgtaggcggt gactgtggac gttcccagca gtgtgccggt taccgtaccg gttgcatctg
     1561 ccagcaatgc acgaccgact cgcggaagct tgccatcgcg taccagaccg gcctgagttc
     1621 ccaccccaat caacgtgccg acggtatcga agacagccag aaagaaaaag accagcacga
     1681 tttcgagata cctgaacgag agcgcgctac caatgtcaag ctggagcagc gtcggtccaa
     1741 gcgacggagg ccgcccgaca atgccggcat atgaccgcac tccagagacc aggccgagaa
     1801 gggttgtcgc aatgattccg atcagaatcg cgccgggcac gcgccgcagc tcgaggacgc
     1861 tgatcagaag gaaccctccc agggcgagga gcggagccgg ctcggtgagc cggcccagcc
     1921 gcaccagact cccggggttc gcgacgatga tgccacccca tcgaaggccc acaaacgcaa
     1981 tcaggagccc gatcccaacc ggaatagcgg ccttgagaca tgccggaatg gcatcgacga
     2041 ttgcttcgcg gaagcggaac gccgccagaa gcacgaagag cgccccggcc aggaagtcgg
     2101 cccccagcgc aacttgccag ggtacgccga gctgggtgac cgcaaacacg aagaagaagt
     2161 tgtggcccat ggccggcgca accgcaatcg gatagttcgc gaccaagccc atgagcccgg
     2221 ttgcgaatgc cgaggcgatg caggtagccg ccatgacggc gccgaaatcc atccctgccc
     2281 cggacagcac agcaggctgg acgaagatga tgtaggagag agctgcaaac gtcgttgcgc
     2341 cagcaacgac ttcggtgcgg atcgtggtgc cgcattccgg cagctggaaa aagcggctca
     2401 ctgacatggc cgaatctgca tcgcgcggta tgagcctgcc aggctgggca gatcggtgag
     2461 ccggctcatt gcgtgagggc aacgcgggcc gtcacttgaa gcattctcaa cagctacccc
     2521 cttcccccct tcctcttatc tccctttctt cccctgcatg ccaaccccca tagtgacagt
     2581 tgacgccttc accgatcgcg ccttcgccgg caatccggct gcagtctgca ttctcgacgc
     2641 gccccgaagc gacgactgga tgcagttggt cgcgcgggag atgaacctgg cagaaaccgc
     2701 attcctggtc cgctgcggcg aggagttcgg cctccgctgg ttcacgccaa gcatcgaggt
     2761 cgacctttgc ggtcacgcga cgctggcgag tgcgcacgtt ctctgggagc agggccacct
     2821 ggctcaatct gacgaggccc gcttccatac ccggagcgga ctgctcaagg ccacccaggg
     2881 cgatggttgg atcacgctgg actttccggc aactccggcc aggccaatcg atccgccgcc
     2941 cgagctggcc cgagcgctcg cagtaccgat ccgattctac gggaaaaccc cgttcgatta
     3001 tctggtcgag gtggagtccg agtcggcggt tcgcgacctc gcaccggaca tggccctgct
     3061 cgcgcagctc aaggggcggg gcgtgatcgt caccagtcgc tcgacgactc cgggtcagga
     3121 ctttgtgtcg aggttcttcg gtcctgccgt cggcattccg gaagacccgg tcacagggtc
     3181 ggcccactgt gcccttggcc cgctctgggg cgagcgcctg aagaaatcgt ctctcgtggg
     3241 atttcaggct tcgccgcggg gaggactggt caaggtgacg ctggcgggag accgcgttct
     3301 cctgggcggc caggcggtga ccgttcttcg aggggaattg ctcgcgtgac tgcgcccaca
     3361 atcgatgggc tccaggcctt ccaagcgtta gaccttcggg tcggacgagt caccagagtc
     3421 gagccgaacg caaaggcgcg aaaaccagcc tatcagctgt ggatcgactt cggaccgctg
     3481 ggtgaaaggc gatcgagcgc gcaactgacc gacggttatc gggcggagga gctggtgggc
     3541 cggctcgtcg tggccgcagt caatctgggc acccggatga tcgcgggcgt gccatccgag
     3601 gttctcgtgc tcggcctccc gaacgacgcg gggcaggtgg tgttgctggc ggtggagagg
     3661 gaggttccgt tgggggggag agtgtattga gaaagcgtga acagtgaacg ggaccctgcc
     3721 ctgccgcccc gttcaacgtt taccctttac cgttcacgct gttcaagctc ttccgtcgcg
     3781 gttgcgctgt ccggctccgc cggcctgacc gcgagcccgc tgaccgcgcc gtccttcgtc
     3841 agggtccact cgatcaccac cggctggcgc gcggcggtga atgcggcggt gcggctgtag
     3901 acgtgctccg gtttgagctc cgcgacgtgc tcgtcgacgg tccgctgctc tcgccccagc
     3961 tgggtgacgg ctcggccggc gaaggctccc agctccgcag cgctgccgaa cacccgctga
     4021 aattcaggtg tgaagcgagc ccagagcttg ccatactcgg ccccgtagag ccagctggtg
     4081 taaatccgtc cccgctccag tgttttaccg gcgactcggt cgccgggcgc gcaggcaagc
     4141 agcgcgagtc caagaggggc cacccggagg atcagtcgag gcatcgtcat gcgcgtctac
     4201 atctcggttg atatggaagg catcgccggc gtggtgcacg aggatcaaac taaccccgtc
     4261 gatccccgtt gcgcagccga gtacgcccgg tttcgcaagc tgatgaccgg ggaagccaac
     4321 gccgccgtcg aaggggccct cgccggcggc gcttcccaag tcgtggtcaa tgatagtcac
     4381 tgggagatga gaaatctgct ccctgaggac ctccatgagg cggcggagct cctgagcgga
     4441 ggacccaaac ggctttcgat gctggaggga atcgaggccg acttcgatgc ggcagtcttc
     4501 attgggtacc acgcgaaggc cggcactgcg ggggcaattc tcgatcacac gatgatggac
     4561 gccattcgcg acgtccggtt aaacggtcgc tccatgggcg agcttgggct caacgctgcg
     4621 ctggcgggcg cccacggagt gccggtcgcc ctggtcagcg gcgaccgggc gacggcc
//
LOCUS       DASRJQ010000127         5731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3017172, whole genome shotgun sequence.
ACCESSION   DASRJQ010000127 DASRJQ010000000
VERSION     DASRJQ010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5731
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3017172"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1223
                     /gene="infB"
                     /locus_tag="VFB89_02960"
     CDS             <1..1223
                     /gene="infB"
                     /locus_tag="VFB89_02960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HZM26290.1"
                     /translation="IVAINKIDLPAANVDKVKRDLLQHNVVLEDFGGNVLSAPISAKK
                     GTGIDHLLEQILLQAELLDLKANPSAVAHGTVLEATLDPGKGPLATVLVQRGTLRVGD
                     NFICGKFSGRVRALYDERGKTVKEAGPSIPVQILGFEGVPSAGDSFAVVTDAVEAREI
                     AQKRQRLEREAQNRRSQRGGTLEDISRALKEGEVTNLRIIIKADQGGPAEALADALAQ
                     LSTAEVRVDVVHRGVGAITESDVLLAKASGAIILGFHVRPDSNARSAAEREQVDVRTY
                     RIIYEAVEDVRNALEGLLKPEEKETVLGEAEVLQLFKVSKVGTIAGCAVRSGVIQRTA
                     KARVIRDGSVVYTGNVSSLKRFKDDVREVREGLECGIGIENFNDVKVGDRIESFRMEE
                     VKRTLQPEAAAGSH"
     gene            1253..1534
                     /locus_tag="VFB89_02965"
     CDS             1253..1534
                     /locus_tag="VFB89_02965"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HZM26291.1"
                     /translation="MFIGVRTWELHLEGCHSLKEKRSVLKPLTAALRRALNVSVAETH
                     HQDLWQRAEIACAAVGSARDVVEETLRAADRLIEDADGVRILDTVTVYR"
     gene            1531..1899
                     /gene="rbfA"
                     /locus_tag="VFB89_02970"
     CDS             1531..1899
                     /gene="rbfA"
                     /locus_tag="VFB89_02970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HZM26292.1"
                     /translation="MKSRRSPSRRPDQVAETLRQVITDALVRGEVRDPRVGLVTVTDV
                     RVTGDLGHARVLVAAPGEDAERLRAIEGLRSAAGFLRSKAARTLSTRTVPELHFELDR
                     GVEHAARINELLDEIKRGSA"
     gene            1911..2762
                     /gene="truB"
                     /locus_tag="VFB89_02975"
     CDS             1911..2762
                     /gene="truB"
                     /locus_tag="VFB89_02975"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HZM26293.1"
                     /translation="MLDKPEGLTSHDVVTRVRRALKTRAVGHTGTLDPFATGLLVVLL
                     GRATRLARFVEAADKTYLATARLGFATTTDDRAGEPLGKTAGLSAVDDTVVRATLKSF
                     EGQSLQRPPAYSAKQVRGQRSYRLARRGAAVQLAEVPVTVRRIELIDWTPPQATFRTT
                     VSAGTYVRALARDLGDRLGVGGHLTALRREAIGTLRVEEAIPLERLDAKTPLVPLRRI
                     LSDLPALELDQDTRAAVRHGRAVVARTHSAATSQGHVVLLGDGGVVAVARSEGDSLRP
                     VVVLEAP"
     gene            2759..3718
                     /locus_tag="VFB89_02980"
     CDS             2759..3718
                     /locus_tag="VFB89_02980"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HZM26294.1"
                     /translation="MTADRSSPLPSFPNGTVLTVGSFDGVHLGHQAVVAEVARRADAA
                     RRPSILVTFEPHPLAVVNPQAAPPLLTSGAERREMLAQLPVDYAYFLRFDHQLAELSP
                     EEFVRDILLARCQMRDLVFGYDHGFGRGRSGDVETLRRLGSQLGFAVDVVPPIDLGGE
                     PISSGRIRRAVAGGDLRTAARLLGRPYRLTGQVVEGERRGRLMGIPTINLGELPPEKL
                     LPPDGVYAVWVEWRGGRVGGMMNQGAKPTFGDGRRSIEAHLFSFEGDLYGEWVRLEWV
                     ERLRDVRRFGSPMELQEQLAHDRARAQAVLAGDRPDPQRASHA"
     gene            3734..5518
                     /gene="secD"
                     /locus_tag="VFB89_02985"
     CDS             3734..5518
                     /gene="secD"
                     /locus_tag="VFB89_02985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HZM26295.1"
                     /translation="MFATIRNRLIVIAVLVIGSVFVLVPRSITLRERDPQTGVMRDTV
                     VKQVPIKRGLDLQGGMHLALELDQSKQVSSDPKRDIELALTVLRKRIDEFGVTEPLIQ
                     KVGDSRIVVELAGITDPARAKAIVQRSAFLEFKITDKTRALEKALPGMDRTLRGMGVK
                     GVDTATQKRPSAVEQLLGTDSIKNLKDTTTKARDTTTKAKEKGKDTTKKIATARVKGG
                     DTTAKGADTAEKAADTTSVPGGVLESLVQPAAAVAGGQVPGEFLVAETAFPRVDSLLN
                     LPEVRRTLPRGVTLRWAASPTSVGVQSYRYLYALDEKSIVTGSNLEDARAQLDPLTNG
                     PIVTFELDRAGGRKFGEETGKHVGDYMAIILDGRVQGRPPVIQSRIGRNGQITLGGKG
                     LQEAQDLALTLKAGALPIPLKIVEERQVGASLGADSIRKGITAGLVGTALVILIMIGY
                     YRLSGVLSILALGLYILFTFGGLAALGATLTLPGLAGLVLSIGIAVDANVLIFERIRE
                     ELLLGKTIRLSVDEGFKHAMNAIIDSNVSTVLTALFLFQFGTGPVKGFAVTLIMGIVA
                     SMISAVFVVRTFYLIWLDRRPTMSTLSI"
     gene            5521..>5731
                     /locus_tag="VFB89_02990"
     CDS             5521..>5731
                     /locus_tag="VFB89_02990"
                     /inference="COORDINATES: protein motif:HMM:NF019187.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26296.1"
                     /translation="MTRFFANANYDFLGVRKYAYAVTAAIILPGLLFLLVRGLNYSIE
                     FTGGTLLQITAKQAVDVGAVRGALDR"
BASE COUNT         1016 a         1787 c         1948 g          980 t
ORIGIN      
        1 tgatcgtcgc catcaacaag atcgatcttc ccgccgcgaa tgtagacaag gtcaagcgcg
       61 acctgctgca gcacaacgtg gtcctcgagg acttcggcgg gaacgtgctc tcggcgccga
      121 tctcggcgaa gaagggaacc ggcatagatc atctgctcga gcagatcctg ctgcaggccg
      181 agctgctcga tctcaaggcg aacccgagtg ccgtggcgca cgggaccgtg ctcgaggcga
      241 cgctcgaccc gggcaagggc ccgctggcga cggtcctggt gcagcggggc acgttgcgcg
      301 tgggtgacaa cttcatctgc ggcaagttct cgggtcgcgt ccgagcgttg tacgacgagc
      361 gcggcaagac ggtcaaggag gccggcccgt caattccggt gcagatcctc ggctttgaag
      421 gcgtgccctc cgcgggcgac tcgttcgcag tcgtcaccga tgcggtcgag gctcgcgaaa
      481 tcgctcagaa acggcaacgc ctcgagcgcg aggcccagaa tcgccgcagt cagcgcggcg
      541 gcacactgga agacatcagt cgcgcgctca aggaaggcga ggtcacgaac ctccggatca
      601 tcatcaaggc ggaccagggc gggcctgccg aggcgctagc cgacgctctg gcgcagctct
      661 caacggccga agttcgagtc gatgtcgtgc accgcggagt aggtgctatc accgagagcg
      721 acgtgcttct cgccaaggct tccggagcca tcatcctcgg cttccatgtg cgccccgact
      781 ccaacgcccg ctcggcggct gagcgagagc aggttgacgt ccgcacctat cgcatcatct
      841 acgaggcggt ggaagacgtc cgcaacgcgc tcgaaggtct gctcaagccc gaagagaagg
      901 agaccgtgct gggcgaagcg gaagtgctcc agctcttcaa ggtcagcaag gtgggtacca
      961 tcgcgggctg cgcagtgcgg agcggggtga tccagcggac cgccaaagcg cgagtgattc
     1021 gcgatggtag tgtggtctac accggcaacg tctccagcct gaagcgattc aaggacgacg
     1081 tgcgcgaggt ccgggaaggg ctcgagtgcg gaatcggtat cgagaacttc aacgacgtca
     1141 aggtaggcga ccggatcgag tctttccgga tggaagaggt gaagcggacg ctgcagccgg
     1201 aggcggccgc cggaagccat tagctgacgg ctatggctaa cggctcctct ccatgttcat
     1261 cggtgtccga acctgggagc tgcatctcga gggttgtcat tcactcaaag agaagcgctc
     1321 cgtgctgaag ccgctgacgg ccgcgctgcg gcgagccctg aacgtctcgg tggctgagac
     1381 gcatcaccag gatctttggc agcgggccga gatcgcgtgt gcggcggtcg gcagtgcccg
     1441 cgatgtggtc gaggaaaccc tccgcgcggc ggatcgcctg atcgaggatg cggacggcgt
     1501 ccgcatcctg gataccgtta cggtataccg atgaaatcgc ggcgctcgcc ctctcgccgt
     1561 cccgatcagg tcgccgagac ccttcgtcag gtgatcaccg acgctctggt tcgcggtgaa
     1621 gttcgcgacc cgagggtggg actggtcacc gtgaccgatg tccgggttac cggcgatctc
     1681 ggccacgccc gggtgctggt cgcggccccg ggcgaagacg cggaacggct gcgtgcgatc
     1741 gagggcctgc gaagcgcggc gggatttctc cgcagcaagg ccgcgcgcac cctgagtacc
     1801 cggaccgtgc cggagcttca tttcgagctg gatcgggggg tagaacacgc cgcccgcatc
     1861 aatgagttgc tggacgagat caagcggggg tcggcctgat cggagccctg ctgctcgaca
     1921 agcccgaggg tctcacatcg cacgatgtag tgacccgggt tcggcgagca ctcaagaccc
     1981 gcgccgtcgg gcacaccggt acgctcgatc cgttcgcaac cgggttgctc gtcgttctgc
     2041 tgggcagggc gactcggctg gcccgatttg tcgaggctgc ggacaagacc tatctcgcga
     2101 cggcgcggct cgggtttgcc acgaccaccg acgaccgcgc cggcgagccg ctcggcaaga
     2161 ctgccggctt gagcgctgtc gacgatacgg tggtgcgggc aacgctgaag agcttcgagg
     2221 gccagagcct ccagcggccc cctgcctact cggcaaagca ggtccggggt caacgcagct
     2281 accgtctcgc tcgccgggga gcagcggttc agctcgctga ggtgccggtg acagtgcgtc
     2341 gaatcgagct gattgattgg acgccacccc aagcgacgtt ccggacgacc gtgagcgctg
     2401 ggacgtacgt gcgagctctc gctcgcgatc tcggcgaccg gctgggcgtg ggcgggcacc
     2461 tcaccgcgct tcgtcgcgaa gcgatcggaa cgctgcgcgt cgaggaggcg attccgctcg
     2521 agcggctcga tgccaaaacc ccgctggtgc cgctcaggcg aatcctgtcg gatctgccgg
     2581 cgctcgagct cgaccaggac actcgcgccg cggtgcgcca cgggcgggca gtcgtagccc
     2641 ggactcactc cgcggccaca agccaaggac acgtggtgct gctgggtgac ggcggagtgg
     2701 tggcggtggc ccgatcggag ggtgattcgc tcaggccggt cgtcgtcctg gaggcaccat
     2761 gaccgctgac cgctcttcgc cgttgccaag cttccccaat gggacggtcc tgacggtcgg
     2821 ttcgttcgac ggggttcacc tggggcacca ggcggtggtc gctgaggttg cccgaagggc
     2881 cgatgcggca agacgcccca gcatcctggt gaccttcgag ccgcatccgc tcgccgtcgt
     2941 gaatccgcag gctgcgcctc cgcttctcac gagcggtgcg gaacgaaggg aaatgcttgc
     3001 ccagctgccg gtggactacg cctattttct gcggttcgac catcagctgg cggagctttc
     3061 acccgaggag ttcgttcgag acattctcct cgcgcgatgt cagatgcggg acctggtctt
     3121 cgggtacgac catggcttcg gcagggggcg gagtggtgac gtcgaaaccc ttcggcggct
     3181 gggttcccag ctcgggttcg cggtagatgt ggtgccgccg atcgatcttg gaggcgagcc
     3241 catttcgagc ggccggattc gtcgtgccgt ggcgggtggc gatctgcgca ctgccgcccg
     3301 cctgctaggg cggccgtatc gactcacggg ccaagtagtc gagggggagc gccggggtcg
     3361 cctgatgggc atccccacca tcaacctggg ggagttgccg cccgagaaac tgctcccccc
     3421 cgacggagtt tacgccgtct gggtagaatg gcggggcggt cgggtaggcg ggatgatgaa
     3481 ccagggcgcc aagccgacgt tcggcgacgg gcggcgctcg atcgaggctc acctgttttc
     3541 cttcgagggt gacctttacg gtgagtgggt acgactcgag tgggtcgaac gcctgcgtga
     3601 cgtgcggcgg ttcggctcgc cgatggagtt gcaggaacag ttggcgcacg atcgggcgcg
     3661 ggcgcaggca gttctcgccg gcgaccggcc ggatccacag cgcgccagtc acgcgtaagc
     3721 tacggaactc acgatgttcg ctacgattcg aaatcgcctt atcgtcatcg ccgtcctggt
     3781 gatcggctcg gtgttcgtcc tggtgccgcg cagcatcacc ctgcgggagc gggatccgca
     3841 aaccggcgtc atgcgcgaca ccgtcgtcaa gcaggtgccg atcaagcggg gactcgatct
     3901 acagggtggg atgcacctcg cactggagct cgatcagtcg aagcaggtgt cctccgatcc
     3961 caagcgggac atcgagctcg cgctcaccgt tctccgcaaa cgcatcgacg agtttggcgt
     4021 gaccgagccg ctgattcaga aggtgggcga cagccgcatc gtcgtcgagc ttgccggaat
     4081 caccgacccc gcccgggcca aggcgatcgt tcagcgaagc gcgtttctcg agttcaagat
     4141 caccgacaag acccgcgcgc tggaaaaggc cttgcccggc atggaccgga cactgcgcgg
     4201 catgggcgtg aagggtgtcg acaccgcaac ccagaagcgc ccctccgccg tcgaacagct
     4261 tctgggaacc gacagcatca agaacctgaa ggacaccacg accaaggcca gggacacgac
     4321 caccaaggcc aaggagaagg ggaaagacac gacgaagaag attgcgacgg cccgggtgaa
     4381 gggcggcgac acgacggcca agggagccga cacggccgag aaggcagcgg atactaccag
     4441 cgtgcccggc ggcgtcctcg agtcactggt ccagccggcc gccgccgtgg ccggcggtca
     4501 ggtgcccggt gagtttctcg ttgcggaaac cgcctttccg cgggtagaca gcctgctcaa
     4561 ccttccggag gttcggcgga cgctcccgcg aggcgtgacc ctccgatggg cggccagccc
     4621 gaccagcgtc ggggtccagt catatcgata cctctatgcg ctggacgaaa agagcatcgt
     4681 caccggcagc aatttggagg acgcccgggc ccagctggac ccgctcacca atggaccgat
     4741 cgtcaccttc gagctggatc gcgcaggggg ccgcaagttc ggcgaagaga ccgggaagca
     4801 tgtaggcgat tacatggcga tcatcctgga cggtcgagtg cagggaaggc cgccggtgat
     4861 ccaaagccgc atcgggcgga acggtcagat tacgctggga ggcaaaggtc tccaggaggc
     4921 gcaggatctg gcgctcacgc tcaaggctgg cgctctgccc atcccgctca agatcgtcga
     4981 agagcggcag gtgggagcga gtctgggtgc tgactcgatc cgtaagggca tcacggcggg
     5041 cctcgtaggg acggcgctgg tgatcctcat catgatcggg tactaccgac tgagtggcgt
     5101 tctctcgatc ctcgcgctcg gcctttacat cctgtttacc ttcggcgggc tggcggcgct
     5161 cggcgcgact ctcacgctcc ctgggcttgc cggcctggtg ttgtccatcg gcatagcagt
     5221 agatgccaat gtgctgatct tcgagcgaat tcgagaagag ctgctcctgg ggaagacgat
     5281 tcgcctgagc gtggacgagg ggttcaagca cgccatgaac gccatcatcg attccaatgt
     5341 cagcaccgtg cttaccgccc ttttcctctt tcaattcgga accggtccgg tgaaaggctt
     5401 tgcggtaacg ctgatcatgg gcatcgtggc ctccatgatc agcgcggtgt tcgtcgttcg
     5461 caccttctat ctcatctggc tggatcgacg cccgaccatg tctacgctga gcatctgacg
     5521 atgacccgct tcttcgccaa cgccaattac gactttctcg gcgtccgcaa gtacgcgtac
     5581 gccgtaacgg ccgcgatcat cctgcctgga ctgctctttc tcctggtgcg cggattgaat
     5641 tacagcatcg agttcaccgg gggtacgctg ctccagatca ccgccaagca ggcggtagat
     5701 gtgggagccg tgcggggcgc gctcgaccgg c
//
LOCUS       DASRJQ010000128         3226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2734809, whole genome shotgun sequence.
ACCESSION   DASRJQ010000128 DASRJQ010000000
VERSION     DASRJQ010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2734809"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /gene="rpe"
                     /locus_tag="VFB89_02995"
     CDS             <1..656
                     /gene="rpe"
                     /locus_tag="VFB89_02995"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HZM26297.1"
                     /translation="IAPSVLSAALERLPDEIRAVEKGGADWIHVDVMDGHFVPNLTFG
                     APMISALARLTDLPLDVHLMVERPERYIEDFAEAGAAVFTFHPEATVHVQRQLAAVRE
                     HGMLAGLALNPGTPLALLQEVVDDVDLVLIMSVNPGYGGQAYIPAATDKIRRCRALLD
                     ARGSRATLEVDGGITLETIGTAWEAGADAFVAGTAVFGTPDPAEATRALRRCCAVRV"
     gene            663..1208
                     /locus_tag="VFB89_03000"
     CDS             663..1208
                     /locus_tag="VFB89_03000"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZM26298.1"
                     /translation="MSKQWLAVLAVLAGLALAATALVKYGPGSEPVAVGSRAPDFRVV
                     DLATGDTVSLRKHYKGKVTLVNIWATWCEPCKIEMPSMQRLYDSLESRGFKIAAVSID
                     ESDPTDVRNFGQALHLTFDILQDRSTAIQQAYQTTGVPESFLLDRNGTIVKRVIGAHD
                     WSSPVNRALVERLLNEGKAES"
     gene            1209..2129
                     /locus_tag="VFB89_03005"
     CDS             1209..2129
                     /locus_tag="VFB89_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26299.1"
                     /translation="MRLPTALTADWPLKLTAVALSVLLWLVASSEEPASGLLQVELRV
                     QPPPGRAVVRALEPLHATAVGSRRELLRLSRDNLFFTRILPDTLTADSVRLDIDPSDV
                     VLPGNPNVRIQDLEPRQVTVELNPVAQRSAPVRPVVRIQSDSGFELVGGIAVVPGEVR
                     LAGPRDGVAAIDSVTTVPLEIARADGPTEERVMVDTTGFGPVRVFPNRVTLTLNVQAI
                     AEKTLWPVPVQLPSGSALKLDRDTVTVRIRGPRYRLAQLTPDSVTVTLVSGAVVGTAP
                     TRVALRVMVPAGLTGKATPDSVSCRRTEHG"
     gene            2122..3180
                     /gene="tsaD"
                     /locus_tag="VFB89_03010"
     CDS             2122..3180
                     /gene="tsaD"
                     /locus_tag="VFB89_03010"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HZM26300.1"
                     /translation="MADRILGIETSCDETSAAVLAVDGARPKLTSLVILSQDVHRIFG
                     GVVPELASRAHVRAIGSVVDRALADAALTLEDIDGIVVTAGPGLVGALLVGVMYGKGL
                     AYSRNIPFLGVNHLEGHLFAPTLEDEELEPPFVALLVSGGHTMLLDVPAWGDYRLLGQ
                     TLDDAAGEAFDKVATLLGLGYPGGAPLERLAREGRPDRFRFPRPLLAELSRTDEPYQF
                     SFSGLKTAVLRAVRASNDLARDRADLARGFQDAALEVLVEKTAHAVEALGYPQAMIGG
                     GVACNQVLMKQLRARLAGIARVSIASPRLNTDNAAMIAAAGAWRLAQGERSRWDLEPR
                     DDLPIPGLRAPDPHRTLA"
BASE COUNT          539 a         1061 c         1056 g          570 t
ORIGIN      
        1 ggatcgcacc aagcgtgttg agcgcagcgc tcgagcggct gccggacgag atccgcgcag
       61 tggagaaagg cggcgcggac tggattcacg tggacgtgat ggacgggcat ttcgtcccca
      121 acctcacgtt cggagccccg atgatcagcg cgttggcccg gctcaccgac ctgccactcg
      181 atgtccacct catggtcgag cgaccggagc gctacatcga agatttcgcc gaggccggcg
      241 ccgccgtctt cacattccac cccgaggcta ccgtgcatgt gcagcggcag ctggcggccg
      301 tgcgcgagca cggcatgctg gctggactcg cgctcaaccc gggcacgccg ctggctctgc
      361 tgcaagaggt ggttgacgac gtggacctgg tcctgatcat gagtgtcaac ccgggttacg
      421 ggggccaggc ctatattccg gcagcgaccg acaagatccg tcgctgtcgg gccctgctcg
      481 acgcgcgcgg gagccgggca actctcgagg tggacggcgg aattacgctc gagacgatcg
      541 gcaccgcgtg ggaagccggc gccgatgcat tcgttgccgg taccgccgtc ttcggaaccc
      601 cggacccggc cgaagcaacc cgcgcgcttc ggcggtgctg cgcggtacgc gtataggaga
      661 ccatgtccaa acagtggttg gctgtgctgg ccgttcttgc cgggctcgcg cttgccgcta
      721 ccgctctggt caagtacggc cctgggtccg agccggtcgc cgtcggctcg cgtgctcccg
      781 atttccgagt cgtcgatctc gccaccggcg acacggtctc cctgcgcaag cattacaagg
      841 gcaaggtgac cctggtaaac atctgggcga cctggtgcga accgtgcaag atcgaaatgc
      901 cttccatgca gcgcctctat gactccctcg aatcccgcgg cttcaagatc gccgccgtga
      961 gcatcgacga aagcgatccg accgatgtgc ggaacttcgg acaggcactc catctgacgt
     1021 tcgacattct tcaggatcgc tccaccgcca ttcagcaggc gtatcaaacg accggtgtgc
     1081 cggagagctt tctgctcgat cgaaacggca cgattgtgaa gcgggtcatc ggcgcccatg
     1141 actggagctc gccggtcaat cgggcgctgg tcgagcgact gctgaacgaa ggaaaagccg
     1201 agtcctgagt gaggctcccc acggcgctca ccgccgactg gcccctcaag ctcaccgccg
     1261 tggccctgtc ggttctgctt tggctcgttg cctcgagcga agagcccgcc agcgggctgc
     1321 ttcaggttga gttgcgggtt cagccgccgc cgggccgagc cgtagtccgg gcgctcgagc
     1381 cgctacacgc gactgcggtg gggtcccggc gagagctgct tcggctgtcc cgcgacaatc
     1441 tgttcttcac caggattctt cccgacaccc tcaccgccga cagcgtccgc ctcgacatcg
     1501 acccctccga cgtcgtcttg ccgggcaacc ccaacgtgcg aattcaggac ctcgagcccc
     1561 gtcaagtcac cgtggagctg aacccggtgg cgcagcgtag cgcaccggtg cggccggtag
     1621 tccgaattca atcggactcg gggttcgagc tggttggcgg tattgcggtg gtaccgggcg
     1681 aagtgcggct ggccggacct cgcgatgggg tagcagcaat cgactcggtg acgacggtac
     1741 cactcgaaat cgccagggcc gacggcccga ccgaggagcg cgtgatggtg gacacgacgg
     1801 gcttcggccc ggtcagggtc tttcccaacc gggtgacgct cacgctcaac gtgcaagcga
     1861 ttgccgagaa aaccctctgg cccgttccgg tgcagctgcc gtccgggtcc gcgctcaagc
     1921 tggaccgcga tacggtgacc gtgcgaattc ggggcccgcg gtatcgactg gctcagctta
     1981 ctccggacag tgtgacggtc acactggtct ccggcgcagt tgtgggaact gcgcccaccc
     2041 gagtcgcact cagagtgatg gtgccggccg gactcaccgg aaaggcgaca ccggacagcg
     2101 tctcctgccg ccgcaccgaa catggctgat cgaatcctcg gcatcgaaac ctcctgcgac
     2161 gagacctcgg cagcggtgct cgccgtggat ggagcgcggc cgaagctgac gagcctggtg
     2221 atcctttccc aggacgttca ccgcatcttt ggcggcgtcg tgcccgagct ggcgagccga
     2281 gcccacgtac gggccatcgg gagcgtggtg gatcgtgcgc tggccgatgc tgcgctcacg
     2341 ttggaggaca ttgatgggat cgtcgtcacc gcgggcccag ggctcgtcgg agcgctcttg
     2401 gtcggggtga tgtacgggaa gggactggcc tactctcgca acattccctt ccttggcgtc
     2461 aatcacttgg aaggccatct cttcgctccc acgctcgagg atgaagaact cgagccgccc
     2521 ttcgtggcgc tcctggtcag cggtgggcac acgatgctgc tggacgtgcc cgcctggggc
     2581 gactaccggc ttctcggtca aacgctggac gacgcggctg gtgaagcgtt cgacaaggtc
     2641 gcgacgttgc tcggtctcgg gtatcccggc ggagctccac tcgaacggct cgcacgcgag
     2701 ggaaggccag atcggttccg ctttccgcgc ccattgctgg cggagctgag tcgcacggac
     2761 gagccgtacc agttctcttt cagcggactc aagaccgcgg tcctccgcgc cgtacgagcc
     2821 agcaacgacc tcgcgcgtga ccgggccgat ctagcccgag gcttccagga cgcggctctg
     2881 gaggtgctgg tcgagaagac tgcgcatgct gtcgaagcac ttggttatcc tcaagccatg
     2941 atcggtggcg gggtggcctg taaccaggtg ctgatgaaac agctccgcgc acggctggcg
     3001 gggatcgcgc gcgtcagcat agcttctccc cgcctgaaca ccgacaatgc cgccatgatc
     3061 gcggccgcag gggcttggcg acttgctcag ggtgaacgaa gccgctggga cctcgagccc
     3121 agggacgacc tcccgatccc cggtctccga gccccggatc ctcacaggac gttggcatga
     3181 cgatctaccc gctcactatt cagcttggcc gtttcgagat cacggg
//
LOCUS       DASRJQ010000129         2096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1044286, whole genome shotgun sequence.
ACCESSION   DASRJQ010000129 DASRJQ010000000
VERSION     DASRJQ010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044286"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VFB89_03015"
     CDS             <1..224
                     /locus_tag="VFB89_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26301.1"
                     /translation="WFLCLYAATGVDLREPLRAGASDDELAALIRRHWTARIDRGAEE
                     RSEAPDRRALYQLAGLRADPHREMHTRGG"
     gene            344..817
                     /locus_tag="VFB89_03020"
     CDS             344..817
                     /locus_tag="VFB89_03020"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HZM26302.1"
                     /translation="MHVVLIEPRIPPNTGSIARLCAATDTPLHLIEPLGFSLSEREVK
                     RAGLDYWDKVDLWVHPGWGDFRNAISRDRCLYFSANATRDYRRAPYRWNSCLVFGNET
                     DGMPVRILEKHPRRCFRIPMPGDVRSLNLANAVSIVLYEGLRQLGVPTPEPTAES"
     gene            949..1872
                     /gene="mdh"
                     /locus_tag="VFB89_03025"
     CDS             949..1872
                     /gene="mdh"
                     /locus_tag="VFB89_03025"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HZM26303.1"
                     /translation="MLSKIAVVGAGNVGATAAQRVAEKSLARTVVMIDVIEGVPQGKA
                     LDQWQSGPIEGFDSRVIGGQRYDAAAGSEIFVVTAGIARKPGMSRDDLVKTNAGIVQS
                     VGEQIKRVAPNAVVIVVSNPLDVMCYVAKKATGFPRERVLGMAGVLDTARYRMFLAEA
                     LDVSVEDIQAMVLGGHGDTMVPLISYTTVSGIPVTQLLAGDKLEAIVDRTRNGGAEIV
                     AHLKTGSAYYAPSAAAVQMVEAIALDKKRVLPCAAWLQGEYGMRDVYCGVPCKLGRLG
                     LQQIIEVKLTDDERRQLEASAEAVRAIQATV"
     gene            1869..>2096
                     /locus_tag="VFB89_03030"
     CDS             1869..>2096
                     /locus_tag="VFB89_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26304.1"
                     /translation="MMPTVQQIAASRRRAIRALWDSSVGKKVVMAVTGAIGIGYVILH
                     MVGNLQVFQGPDRLNAYAALLHGPLNELLWVA"
BASE COUNT          361 a          653 c          697 g          385 t
ORIGIN      
        1 cctggttcct gtgcctgtac gcggccaccg gcgtcgatct tcgcgagcca ctccgcgccg
       61 gcgcctccga tgacgagctg gctgccttga ttcgcaggca ctggaccgca cgtatcgatc
      121 gcggggcgga ggagcgaagc gaggcgccgg accggcgtgc cctgtaccag ctggctggac
      181 ttcgcgccga tccgcatcgc gagatgcaca cgcgtggcgg ctaggtggcg gtgaaaggga
      241 gaagggggga aggggagaaa ggggtgcgtc cagcttccag cgccccacgg cgcctccctc
      301 cttccccctt tctccccttc tccctttccc cggccgtgta gccgtgcacg tcgtcttgat
      361 cgagccacgc attcctccaa acaccgggag catcgcccgg ctctgcgcgg ccacagatac
      421 accgctccat ctcatcgagc cgctcggatt ctcactgagc gaacgcgagg taaagcgcgc
      481 cggcctggac tattgggaca aggtagatct ctgggttcac cccggctggg gagactttcg
      541 gaacgccatc tctcgggatc gttgccttta cttctccgct aatgccaccc gcgattaccg
      601 gcgcgcacct tatcgctgga acagctgcct ggtcttcggc aacgagacgg acggaatgcc
      661 ggtgcggatt ctcgagaagc acccccggcg ctgtttccgg attcccatgc ccggcgacgt
      721 tcgcagcctc aacctggcaa acgcggtgag catcgtgctg tacgaaggtc tccgtcagct
      781 cggcgttccc actcccgagc ccaccgctga gtcctgagtc ggcgtgatgc gccgcggtgt
      841 agtacgggac cacttcgcgg tatacttttc tgccctcgat ccactgagcc acgttgtcgc
      901 aaccgggtcg gcggctgcca ccggctaaac gcaaacctgt gagatctgat gctgagcaag
      961 attgccgtgg tcggagcggg aaacgtcggt gcaacggcgg cgcaacgcgt ggccgagaag
     1021 tcgctagccc gcacggtggt catgatcgac gtgatcgagg gcgtgcccca gggcaaggca
     1081 ctcgatcagt ggcagtccgg tcctatcgaa ggcttcgaca gccgggtgat cggcggtcag
     1141 aggtacgacg cggcggccgg gtcggaaatc ttcgtggtga cagccgggat tgcgcggaag
     1201 ccggggatga gccgcgacga tctggtcaag accaacgcgg gcattgtcca gtcggtgggc
     1261 gagcagatca agcgagtcgc ccccaatgct gtcgtgatcg tggtgtcgaa tccgctcgac
     1321 gtcatgtgtt acgttgccaa gaaggcgacc gggttcccgc gggagcgggt gctgggcatg
     1381 gccggtgtcc tggacacggc gcgatatcgg atgttcctgg ccgaggcact ggatgtgtcg
     1441 gtcgaggaca tccaggcaat ggtgctcggc gggcacggcg ataccatggt tccgctgatt
     1501 tcgtacacca ccgtctcggg aattcccgtg acccagctgc tcgccggtga caagctcgaa
     1561 gcgatcgtcg atcgcactcg aaacgggggc gccgagatcg tcgcacacct gaagactggc
     1621 tccgcgtatt acgcccccag tgccgcggcg gtgcaaatgg tcgaggcgat tgcgctggac
     1681 aagaagcggg tgctgccctg cgctgcctgg cttcagggcg aatacggtat gcgcgatgtc
     1741 tactgtgggg ttccctgcaa attgggtcgc ctgggtctgc aacagatcat cgaagtcaag
     1801 ttgaccgacg acgaacggcg gcagctcgag gcatcggctg aagcggtccg cgcgatccag
     1861 gcaacggtat gatgccgacg gttcagcaga tcgccgcctc ccgccgccgg gctatccggg
     1921 cgttgtggga cagctcggtc ggcaagaaag tcgtcatggc cgtcaccggg gcgatcggga
     1981 tcggctacgt gatcctgcac atggtcggga atctccaggt atttcaaggc cccgaccgac
     2041 tcaatgctta tgccgcgctg ctgcatgggc cgctgaacga gctgctgtgg gtggcc
//
LOCUS       DASRJQ010000130         4482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3858623, whole genome shotgun sequence.
ACCESSION   DASRJQ010000130 DASRJQ010000000
VERSION     DASRJQ010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3858623"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="VFB89_03035"
     CDS             <1..1155
                     /locus_tag="VFB89_03035"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM26305.1"
                     /translation="SGTVDDVFDLQERLSRQIVEALRITLTPPEDRGIAARPIADVRA
                     FEYYQRARQEYYRYTPEGMAAARALAEHGLAVVGPNEALYGMLGTVYAWSPVVLGGDE
                     ESTLREAEACAHRAFELNPHSAQGLSIMGQVAYRRGQPGEAVRLLTQACAADPNNPDA
                     MHQLGGAYLLGGRIGPMREVLTRLVELDPLTPSNHCLLGLSYLLGGDPAGALPAHRRA
                     VELDPRSTICRVCAGVALVAAGREAEAATHFGWLEQQPSDDHLARVAMRFRRGLAGNR
                     AAVLSPPSAAERAMAESDEYWSYLMAGAYALVKETNEALRWLEHTVSVRGWVDYVYFT
                     RHDRFLESLRPTRRFQELMASAQERYARFTNAGAPAPPLPETQIAAAERS"
     gene            1371..1916
                     /locus_tag="VFB89_03040"
     CDS             1371..1916
                     /locus_tag="VFB89_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HZM26306.1"
                     /translation="MTRWLQAARDGEPGALDRLVPLIYDDLRLLARRQLGREYGDRTL
                     NPTALVHEAYLKLGAGAKVAADRAHFLAIAARAMRQVLVDRARDRKAVKRGGGAWERT
                     TLTDGAWVGEFDPDGMLALDEGLARLEPRQRQVVECRFFGGMEEQEIAEALGVSERTV
                     HRDWVKARAWLYRYFYPETTV"
     gene            1913..>4482
                     /locus_tag="VFB89_03045"
     CDS             1913..>4482
                     /locus_tag="VFB89_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZM26307.1"
                     /translation="MNSPLEQQIRFCTSGDDVRIAYATVGSGPPLVKVANWLSHLEFD
                     SHSPVWRHWIRELSRNHMLVRYDERGCGLSDWAIEEFSLDAWVRDLETVVDALSLERF
                     PLLGISQGGPIAIAYTARHPERVSRLILYGSYARGRLQRKLSDREREEAELLLRMITV
                     GWGTDHTAFRQVFTSLFIPDGTPEQAQWFNELQRVSTTPENAARMVATFYSLDVRALA
                     PRLDTPTLVLHAADDMRVSFEEGRLLASLIPGARFVPLDGRNHILLESEPAWPRFLHE
                     VRKFLGVEDSRDVSPVARTPSIPVATTLRRRVEAVFDEVVDLAPADRPAFLARACADD
                     VECRREVERMLELAEQSGLTAKLAGAVARPAAYRPAATPGQTLSQYEIVDQLGGGGMG
                     VVYKALDRRLQRFVAVKFLPPYLSAEKELKLRFLQEAKAIASFDHPNICAVLEVEELK
                     DGQLFMVMPYYEGETLKEKIARGPLRVEQTLAYASQIAAGLAHAHAAGVVHRDVKPAN
                     LMVTADDRLKILDFGVAKVSDLNLTQAGAVMGTPFYMSPEQTCGDPVDGRTDLWALGA
                     VLYEMLTGRPPFQADTAAALFFVIRHREPPSIRALRPEVPPALERIVYRLLEKEPGRR
                     YGDARAVVAELEHLRRLQAIDSSSSLGMPGDPTPTVTGTDHLQRARAAFARSAWRDAY
                     DSLSAADAAGRLETEDLERLAEAAWWLSDGTACVRARERAYRGYVQRGELRGAATVAL
                     ALAEDHFHRLARSVGQGWLLRAERHLEGLPEVSVHGWLYRLKCVIALEAEGQPEQAME
                     HADRALEIARRVRDADLEALALQDRGRALVALGRVKDGMALIDEAMTAASAGELTPRT
                     T"
BASE COUNT          701 a         1476 c         1573 g          732 t
ORIGIN      
        1 tcgggcaccg tcgatgacgt gttcgacctc caggagcggc tgtcgcggca gatcgtggag
       61 gcgctgcgga tcacgctgac cccaccggag gatcgcggga tcgcggcccg gccgatcgcc
      121 gacgtccgtg cgttcgagta ctaccagcgc gcccgacagg agtactaccg gtacaccccg
      181 gagggaatgg cagccgcacg agcgctcgcg gagcacggtc tcgccgtggt gggccccaac
      241 gaggcgctct acgggatgct gggcaccgtg tacgcctggt cgccggtagt gctcggcggc
      301 gacgaggaat caacgctgcg ggaagcagag gcgtgtgccc accgggcgtt cgagctgaac
      361 ccccactcgg cgcagggtct ctcgatcatg gggcaggtcg cgtaccgtcg cggccagccg
      421 ggagaggcgg tccggctgct gacccaggcc tgcgccgccg accccaacaa tcccgacgcg
      481 atgcaccagt taggcggggc ctatctgctg ggcgggagga tcgggccgat gcgcgaggtg
      541 ctgacccggc tggtcgaact ggacccgctg acgcccagca atcactgctt gctggggctg
      601 agctacttgc tggggggcga tcccgctgga gcgctgccgg cacaccggcg cgcggtcgag
      661 ctggatccac ggagcacgat ctgccgggtc tgcgccggcg tcgcgctggt ggcggcggga
      721 cgcgaggccg aggcagcaac tcacttcggt tggctggaac agcagccttc ggacgatcac
      781 ctggcaagag tggcgatgcg attccggcga ggtctcgccg gcaatcgggc agcggtgctg
      841 tcccctccca gcgcagcgga gcgcgcaatg gcggagtcgg acgagtactg gtcatacctg
      901 atggccgggg cctacgcgct ggttaaagag acgaacgagg ccctgcgatg gctggagcat
      961 acggtctcgg tccggggctg ggtcgactat gtgtacttca cgcgacacga ccgttttctc
     1021 gagagcctgc gcccgacccg acgcttccag gaactgatgg cgtcggcgca ggagcgatac
     1081 gcgcgattca ccaacgccgg cgctccggcg ccaccgctcc ccgagacgca gatcgcggcg
     1141 gccgagcggt catgagcctg gctggtcgag gcttgcgcgt ccctagcccc ctgcgaaaga
     1201 tctccagtcc cggctgagcg atcccgcggt tcttcgcgag ccgcgtctgc cagcgggctg
     1261 cgtccccggc ggcatcttgt gtggcctctc gttggtccgg caaattggtc ggtgccccct
     1321 gccccccagc tggcgcgcct atgggcggtc tcgatcccga tgttggtgaa gtgacacggt
     1381 ggttgcaggc cgcgcgcgac ggggagcctg gcgcgctcga ccgcctcgtc cctctcatct
     1441 acgacgactt gcgcctgctc gcccggaggc agctgggacg ggagtatggc gatcgcacgt
     1501 tgaaccccac cgccctcgtg cacgaggcct acctcaagct cggtgcgggg gccaaggtgg
     1561 ccgcagaccg ggcccacttt ctcgccattg ccgcccgcgc catgcgtcag gtcctggtcg
     1621 accgcgcccg tgatcggaag gcggtcaagc gcggcggcgg agcttgggag cgcactacgc
     1681 tgaccgacgg tgcgtgggtg ggggaattcg accccgatgg gatgcttgcg ttggacgagg
     1741 gtcttgcgag actcgagccg cgccagcggc aggttgtgga gtgccggttt ttcggcggaa
     1801 tggaagaaca ggagattgcc gaagccctgg gtgtctccga gcgcaccgta caccgcgact
     1861 gggtgaaagc tcgcgcgtgg ctctaccgct atttctatcc cgagacgacc gtgtgaactc
     1921 gcctctcgag cagcagatcc gtttctgcac ctcaggcgac gacgtgcgca tcgcgtacgc
     1981 cacggtcgga agcgggccac cgctggtcaa ggtcgccaac tggctcagcc atctcgagtt
     2041 cgacagccac agcccggtct ggcgccattg gatccgggag ctctcccgca accacatgct
     2101 ggtccgctat gacgagcgtg gctgcggact ctccgactgg gcaatcgagg agttttccct
     2161 cgacgcctgg gttcgggatc tcgagaccgt ggtggacgct ctctcgctgg agcggttccc
     2221 gctgctcgga atctcccagg gtggccctat cgcgatcgcc tacacggcgc gccacccgga
     2281 gcgggtgagc cggctcatcc tttacgggtc ctatgcgcgg ggccgcttgc agcggaaact
     2341 ctccgaccgc gagcgcgagg aggcggagct gttgctgcgg atgatcaccg tcggctgggg
     2401 cacggaccac accgcctttc gccaggtgtt caccagcctg ttcatccccg acggcacccc
     2461 cgagcaggcg cagtggttca acgagctcca gcgggtctcg accacgccgg agaacgccgc
     2521 tcggatggtc gccacgttct attccctcga cgtccgcgcg ctggcgccga ggctcgacac
     2581 gccaacgctt gtcctccacg cagccgatga tatgcgggtg tcattcgaag aaggtcggct
     2641 tctggcctcc ctcatcccgg gcgcgcggtt cgtgcccctc gacggccgga accacatcct
     2701 gctcgagagc gagcccgcat ggcctcgctt cctgcacgaa gtcagaaagt tccttggggt
     2761 tgaggactcc cgcgacgtgt cccctgtcgc ccgcacaccc tcgatccccg tcgccaccac
     2821 cctgcggcgg cgggtcgaag cggtattcga cgaggtcgtg gacctagccc ccgctgatcg
     2881 cccggcgttc ctggcccgag cctgcgccga tgacgtcgag tgccggcggg aggtcgagcg
     2941 gatgttggag ctggcggagc agagtggcct gaccgcgaag ctcgccgggg ctgtggccag
     3001 acccgccgcg tatcggcccg ccgcgacgcc cggacagaca ttgtcgcagt acgagattgt
     3061 cgaccagctc ggcggcggtg ggatgggcgt ggtgtacaag gccctcgacc ggcgcctcca
     3121 gcggttcgtg gcagtcaagt tcctcccacc ctacctcagc gcagagaagg agctgaagct
     3181 ccgcttcctg caggaggcca aggcgatcgc ctccttcgac cacccgaaca tctgcgcggt
     3241 gctcgaagtc gaggaactga aggacggaca gctcttcatg gtcatgccct actacgaggg
     3301 cgagactctg aaggagaaaa tcgcccgcgg acctctccgc gtggagcaga cgctggccta
     3361 cgcatcccag atcgctgccg gcctcgctca cgcacatgcg gccggcgtgg tacaccggga
     3421 cgtgaagcca gccaacctca tggtgacggc tgacgaccgg ctcaagatcc tggatttcgg
     3481 ggtggcgaaa gtgtcggacc tgaatctgac ccaggcgggg gccgtgatgg gaacgccgtt
     3541 ctacatgagc cccgagcaga cgtgcggcga tcccgttgac gggcggacgg atctgtgggc
     3601 gcttggcgcg gtgctctacg agatgctcac cggtcggcca ccatttcagg ccgacacggc
     3661 ggcggcgctg ttctttgtca tccggcaccg agagccaccc agcataagag cgctccgccc
     3721 tgaagttccg ccggcgctcg agcggattgt gtatcgcttg ctggagaagg aaccgggccg
     3781 ccgctacggt gatgcccgcg cggtggtggc cgagctggag cacttgcgtc gactccaagc
     3841 aatcgattcg tcgtcgagcc ttggcatgcc gggagatccg acgccgaccg tcaccgggac
     3901 ggaccatctc cagcgagcga gggctgcctt cgcacgcagc gcatggcggg acgcctacga
     3961 cagcctcagc gcggcggatg ccgctggcag gttggagacc gaagacctgg agcgcctggc
     4021 ggaggccgca tggtggctgt cggatggaac ggcgtgcgtc cgcgcgcggg aacgggccta
     4081 tcgcggatac gtgcaacgtg gcgagcttcg cggtgcggcg acagttgcgc ttgccctggc
     4141 ggaggaccat ttccatcgct tggcccggtc cgtcgggcag ggatggcttc ttcgggccga
     4201 gcggcatctg gaggggcttc ccgaggtgtc cgtacacggc tggctctatc ggctgaagtg
     4261 cgtgatcgcg ctggaggcgg aagggcagcc ggagcaggcc atggaacatg cggaccgggc
     4321 gctcgagatc gctcgccgag tccgcgacgc cgatctcgag gcgctggcgc tgcaggatcg
     4381 tgggcgcgcg ctggtggcgc ttggacgcgt gaaggacgga atggcgctca tcgacgaggc
     4441 gatgacggct gcgagcgccg gcgagctgac gcctcgtacc ac
//
LOCUS       DASRJQ010000131         3634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_4141248, whole genome shotgun sequence.
ACCESSION   DASRJQ010000131 DASRJQ010000000
VERSION     DASRJQ010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4141248"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VFB89_03050"
     CDS             <1..461
                     /locus_tag="VFB89_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26308.1"
                     /translation="LERLAGFSPIPRIENQHLALALAESLRTELGADESLVSDHYGWG
                     VTQYVALLTRLPRSRIYLAPGAPNEQIDADSLAAFLTQHPCGVLIAETESRFSRRLEL
                     RPETRNAIAGKTSLRLLPTQEVAWPGRHPAILMVFRYSVRSDGDVCPPRE"
     gene            complement(501..1397)
                     /locus_tag="VFB89_03055"
     CDS             complement(501..1397)
                     /locus_tag="VFB89_03055"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HZM26309.1"
                     /translation="MRAPLLLRMFRAALKGWWDDNVPRLGASLAFYTLFAIAPVLVVA
                     IAVAGAVFGGDAVRGHIVNQIDGLVGRVGARAVQSLLQGASRPRENLIASVVGGVTFI
                     LTSTGAFLELQTALNTIWRVKPVPGLNLKAFLMDRLRSFGLVISMGFLLMVSLAVSAA
                     LAAVAGWLQQWAPGMPVFLAVFDLVLSVAVFTALFAILYRFLPDVKLGWHDVTTGAFV
                     TALLFTIGKQLIGLYLGQGSTASSYGAAGSVMVLLLWVYYASQIVLLGAEFTRVYTRR
                     EGSKPEPETFAEKDLNAPRKVV"
     gene            complement(1397..1795)
                     /locus_tag="VFB89_03060"
     CDS             complement(1397..1795)
                     /locus_tag="VFB89_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26310.1"
                     /translation="MKRFCLLFLVMIGPITGRALAQAAAVGGGGCPEPIRATPDSMVA
                     ADSGPTPQRLTAMGGGPRDAPHRAVIAHFVVDAAGQVDTSTISVEHTNDAAWIERLRQ
                     ALADAVYKPAYNDGCPVARWSTFAVLWTKP"
     gene            complement(1890..3026)
                     /locus_tag="VFB89_03065"
     CDS             complement(1890..3026)
                     /locus_tag="VFB89_03065"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZM26311.1"
                     /translation="MSIIAPRFPSLLLFSPSALLGCLALAGSFAFPRNLPAQARDAGA
                     RMERVAENVYAIIHDDATDEWPHGNTGVVVTDAGVLVIDATYLPSLARADIALIRSVT
                     RQQVRYLVYTHWHFDHNDGAVAYTEAFPGLTVVSERESAQFLALNATWWSRMSTAPNS
                     ARRAALAALEKAVADQRDSTGRPLSADTLSRTRKAVAQRKAELNELASLKVITPNLVF
                     DRELTLELGGRRIELRDRGRANSPHDVTIYLPDARVLFSGDVLVQSPLPYVGASWPVP
                     WIGVLRELETIPITALVPGHGPVMHDHSYTRQVRELLEAATSRVAAMAREGKTLEQIQ
                     ATLTLDDIRARTPAWQAPDLKADWETNTKALVERTWRGVRGQGG"
     gene            complement(3065..>3634)
                     /locus_tag="VFB89_03070"
     CDS             complement(3065..>3634)
                     /locus_tag="VFB89_03070"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZM26312.1"
                     /translation="GCEITALRLKLEWIQPVLGLVPGDHDDADHDLPAILPNFGAPLF
                     DHLVETRTSTEAAGLLESAVARQLRGTRVEYAKVPGGALDLVRRARGCVPIERVARHM
                     GVSMRHLRRVVRQAAGVSLKAYARAQRIVGAMTAADRENRPQWARLAADSGFYDQSHL
                     VRECRALCGMTPVEVHRERRLEAELSNRG"
BASE COUNT          670 a         1157 c         1206 g          601 t
ORIGIN      
        1 ggctggagcg gctggcaggc ttctcgccga ttccccgaat tgagaatcag cacctggcgc
       61 ttgcgcttgc cgaatccttg cgaactgagc tcggagccga tgagtcgctg gtgagcgatc
      121 actacgggtg gggcgtgacc cagtacgtgg cgctgctgac ccgactgccg cgcagccgca
      181 tctatctggc acccggcgca cccaatgagc agatcgacgc ggattcattg gcagcctttc
      241 tcacccagca tccttgtggg gttctgattg cggaaaccga gtcgcggttt tctcgccgac
      301 tggagctgag gccggaaacg aggaacgcca ttgccggaaa gacgtcgctg cggctccttc
      361 cgactcaaga agtcgcttgg cccggtcgtc acccggccat cctcatggtg ttccgctatt
      421 cggtcaggag cgacggcgac gtctgtccgc cgcgagagta agtgccccgc cgatcaccaa
      481 gccagcgacg aggccttcgg tcagacgacc tttcgcggcg cgttcaaatc tttttcggcg
      541 aacgtctccg gctccggctt ggatccttcg cgcctggtgt aaacccgggt aaactcggcg
      601 ccgagcagaa cgatctgcga cgcgtaatag acccagagca ggagcaccat caccgacccg
      661 gcggcgccgt aactggatgc agtgctgccc tggcccagat agagaccgat gagctgcttc
      721 ccgatcgtga atagcagagc tgtcacaaat gcgccggtcg tgacatcgtg ccagccgagc
      781 ttaacgtcgg gaaggaagcg gtagagaatc gcgaacagcg cggtgaagac ggcgaccgag
      841 agaacgagat cgaacacggc caggaaaacc ggcatcccgg gcgcccactg ctggagccat
      901 ccggccaccg cggcgagtgc cgcgctcacc gcgagcgaga ccataagcag gaagcccatc
      961 gaaatgacca gaccgaagga gcggagccgg tccatcagaa aggccttcag attgagtcct
     1021 gggaccggct tcacgcgcca gatcgtattg agcgcggtct gcagctcgag gaaggcgccg
     1081 gtcgatgtca ggatgaacgt gaccccgccg accacggacg ctatcagatt ttccctcggc
     1141 cggctggcgc cttgcagcag actctggacc gctcgagcgc cgactcgtcc gaccaggccg
     1201 tcgatctgat tcacgatgtg cccgcgcacc gcgtcgccgc cgaagacggc gccggcgacc
     1261 gcaatggcga cgacgagtac aggagcaatg gcgaacaggg tatagaatgc gagcgacgcg
     1321 cccaggcgag gcacattgtc gtcccaccag cccttgagcg ctgctcgaaa catccggagc
     1381 aacaggggcg cccgcattat ggcttggtcc agagcaccgc gaaggtggac cagcgagcta
     1441 ccgggcagcc gtcgttatag gcgggcttgt agaccgcatc cgccagggct tgcctgagcc
     1501 gctcgatcca ggcggcgtcg ttggtgtgtt cgacggatat ggtcgatgta tcgacctggc
     1561 cagcggcgtc caccacgaag tgcgcgatca cggcccgatg cggtgcgtcc cggggaccac
     1621 cgcccatggc ggtcaggcgt tggggcgtgg ggccgctgtc agccgcaacc atcgagtccg
     1681 gcgtggcccg gattggttcc gggcaaccgc cgccgcccac agcagcagct tgggcgagcg
     1741 cgcggcccgt aatcgggcca atcatcacca ggaagagcag gcaaaaacgc ttcacggggc
     1801 aaatctgacg gcctgccgac ccgtgcgtcg tgggcaaagc tcacaagaca gcgatgacgc
     1861 cggttacctg taaccctcca gtcgccgaat cagcctccct gtccccgtac gccccgccag
     1921 gtccgttcca ccagggcttt ggtgttcgtt tcccagtcgg ccttcaggtc cggcgcctgc
     1981 cacgccggcg tccgggcccg aatgtcgtcg agcgtgagcg tggcctgaat ctgctccagc
     2041 gtctttccct ctcgcgccat ggcagccacg cgtgacgtcg ccgcttcgag cagttcgcgc
     2101 acctggcggg tatagctgtg gtcgtgcatg acggggccat gcccagggac gagagcggtg
     2161 atgggaatcg tctctagctc gcgcagtacc ccgatccagg gaaccggcca tgacgctcct
     2221 acatacggga gaggagactg caccagaacg tcaccgctga agagtacgcg tgcgtcgggg
     2281 aggtagatgg tcacgtcgtg cgggctgttg gcccgccctc gatcgcgcag ctcgatgcgg
     2341 cgtccgccaa gctcaagagt cagctcccgg tcgaacacca ggttcggcgt gatgaccttg
     2401 agcgaagcca gctcgttcaa ttcggccttg cgctgtgcga cggctttcct ggtccgactg
     2461 agggtgtccg ctgatagtgg ccggccggta ctgtcgcgct gatcggcaac cgctttctcg
     2521 agcgcggcca gggcggcgcg gcgcgccgag ttgggcgcgg tggacatccg agaccaccag
     2581 gtggcattca aggcgagaaa ttgggcggat tctcgctcgc tcacgacggt caacccggga
     2641 aatgcttcgg tgtacgcgac ggctccatcg ttgtggtcga aatgccagtg ggtgtagacc
     2701 agatagcgaa cctgctggcg tgttaccgag cgaatcagcg cgatgtcggc ccgggctaga
     2761 gagggcaagt aggtggcgtc gatcaccagc acacccgcgt cggtcaccac gactccggtg
     2821 tttccgtggg gccactcatc cgtagcatcg tcatggatga tcgcgtagac gttctcggcc
     2881 acgcgctcca tgcgggctcc cgcatcacgg gcctgggccg gcaggttgcg ggggaaggcg
     2941 aaagaaccgg ctaaagcgag acagccgaga agcgcggaag gggagaaaag gagaaggctg
     3001 gggaaccggg gtgcgatgat ggacatggcg tcgggaagtg ttgcccgacg ttaccgtcct
     3061 gcgatcagcc gcgattggat aattcggcct cgagccgccg ttcccgatgc acctcgaccg
     3121 gtgtcatccc gcagagtgcg cgacactccc gaaccaggtg tgactggtcg tagaacccgg
     3181 agtcggccgc gagccttgcc cattgggggc ggttctctcg atccgccgcg gtcatggcgc
     3241 caacgattcg ctgagcgcgg gcgtatgcct taagcgacac gcccgcggcc tgccgaacca
     3301 cccgccgcag gtggcgcatc gatactccca tgtgtcgcgc gacccgctcg atcggaacgc
     3361 agccgcgggc tcgccggacc agatcgaggg cacctcccgg caccttcgcg tattccactc
     3421 gggtgccgcg gagctggcga gccactgcgc tctcgagcag gccagccgct tcggtagagg
     3481 tccgggtctc taccaggtgg tcgaacaagg gggccccgaa gttgggaaga atcgcgggaa
     3541 ggtcgtggtc ggcatcgtcg tggtcgcctg ggaccaggcc gaggacaggc tggatccact
     3601 ccagcttgag ccgcaaagca gtgatctcgc agcc
//
LOCUS       DASRJQ010000132         4146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1605623, whole genome shotgun sequence.
ACCESSION   DASRJQ010000132 DASRJQ010000000
VERSION     DASRJQ010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1605623"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..264
                     /locus_tag="VFB89_03075"
     tRNA            188..264
                     /locus_tag="VFB89_03075"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:222..224,aa:His,seq:gtg)
     gene            311..387
                     /locus_tag="VFB89_03080"
     tRNA            311..387
                     /locus_tag="VFB89_03080"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:345..347,aa:Arg,seq:ccg)
     gene            447..522
                     /locus_tag="VFB89_03085"
     tRNA            447..522
                     /locus_tag="VFB89_03085"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:480..482,aa:Lys,seq:ctt)
     gene            complement(731..1396)
                     /locus_tag="VFB89_03090"
     CDS             complement(731..1396)
                     /locus_tag="VFB89_03090"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZM26313.1"
                     /translation="MAGPQPRSPTTRALLGGLRELGYVYGEHFVTEPRGGEGKPERFP
                     ALAAELVRRQVDVIVSAGPPAMLAAVKQATSTIPETAARALGRKLLPLEILDAGESAG
                     AFKTATAAGAGALLVFPGGIPDAHARRIAQLAAASRLPAMYAFRYYVEAGGLISYGAD
                     LIANNRRAAAFVDKILKGAKPADLPFEQPAKFELVINLGAAKLLGLTIPQSLLLRADQ
                     VVR"
     gene            complement(1610..2584)
                     /locus_tag="VFB89_03095"
     CDS             complement(1610..2584)
                     /locus_tag="VFB89_03095"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZM26314.1"
                     /translation="MRSRSSAALLLLSALVILAAPLVTEAQPAGKLHRVGVLTGSANV
                     EALRQGLRDLGYREGTDVVILHQDVEGRFDRLDAAVAHLMRQRVDVIVAGGSESVQTA
                     RRATASIPIVMTMVGDAVGQGFIASFAKPGGNITGLSNVVDDLAPKWLELLRTVSPGI
                     KRVAVLWNPPQPAHEHLLKTLETGAASLGLELQPLAVRTADDLDGALVAAKRERAGGL
                     TMLGSVIHFRNLRRIADLAKRSKLPAVSWTREFVAFGGLMSYGVNQVGQYRRAAYFVD
                     KILKGAAPATLPVEQPTSFELVINAKTATALGLKIPHSLLLRADQVIE"
     gene            complement(2709..3704)
                     /locus_tag="VFB89_03100"
     CDS             complement(2709..3704)
                     /locus_tag="VFB89_03100"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZM26315.1"
                     /translation="MALRFGGACLLFALSLVLLGAPRAADGQRAERVYRLGYLAMSSG
                     PDGERLDSLRQGLRDLGYIEGRNIVFEIRYAEGRPERLPDLAAELVRRKIDLIVTATG
                     AAAVAAKKATQSIPIVMVGSGDAVRQGLAVSLARPGGNVTGFTMISPELSRKRLELLR
                     ELLPKLSRVGVLGCRRGTPVTEQEWIETRAAADVLGVQLSPLAASGREDLVRAFASAA
                     NQRVQAVLGFDCPRLLPSAALIAELSLKHRLPAVYASQRYPQAGGLMSYSPSVADAPR
                     RAATYVDKILRGAKPSELPIEQPTKFELVINRKTATALGLTIPQPLLLRADRVID"
BASE COUNT          722 a         1343 c         1377 g          704 t
ORIGIN      
        1 gcctgcgccc gccggccgag ggcctacccg ccatgcgcga gctgctctcc ctctccccac
       61 cggggagagg gcagggtgag ggggccacct cgcggggcgg ttgatcagct ggtccaagcc
      121 cgcaaccccg cttgccttcc cccaatttcc ccgctaccct accctcctgc tgtcgctcca
      181 ggacgtggtg accgtagctc aattggttag agcactggac tgtggctcca ggggttgggg
      241 gttcgattcc cctcggtcac cccaccgcct ggaggcgcag cgaaaggcgc ggcgagcgcg
      301 cgcggggcgg gcgcccatag ctcagttgga tagagcgttg gcctccggag ccaaaggtca
      361 gaggttcgaa tcctcttggg cgcgccacgc tttcgcgatc ggagcgctcg cgggcgggaa
      421 cacggcagca caggtgggac tgatacgggc cgttagctca gttggtagag cagctgactc
      481 ttaatcagcg ggtccaaggt tcgagtcctt gacggcccac caaaatcagt aggttagcga
      541 gcgcctccac gaggcgctcg cgcctgctga cggtctgccg acgaaatcca gccgattccg
      601 agttcttgcc gcaggtcgtt caatgacgtg ttgggataaa tcgggacatt tcgggacgat
      661 gccgcgttga tcgttgatca acgggtccct agttcacggc gcgatctggc aacatggcga
      721 tgaatcatag ctatcggacg acctggtccg cgcgcaggag cagggactga gggatcgtca
      781 gtccaagcag cttcgccgcc ccgagattga ttaccagctc gaacttggca ggctgctcga
      841 agggcaggtc ggccggtttg gcacccttga ggatcttgtc gacaaaggcc gccgcacgcc
      901 gattgttcgc gatgagatct gcgccatagg atatgaggcc acccgcctcg acgtagtacc
      961 ggaaggcata catcgcgggg agcctgctcg cggcggcgag ctgcgcgatc cgccgggcgt
     1021 gggcgtcggg gatgccgccg gggaacacga gcagagcgcc ggcgcccgcc gccgtcgccg
     1081 tcttgaaggc gccggcgctt tccccagcat ctagaatctc gagcggcagt agctttctcc
     1141 cgagagctcg agcagcggtc tcggggatcg tcgaggtcgc ctgcttgacc gcggccagca
     1201 tcgccggcgg cccggcggag acaatgacgt ccacctggcg gcgaaccagc tcggccgcga
     1261 gggcggggaa gcgctccggt ttgccttcgc cgccccgagg ctccgtcacg aagtgctctc
     1321 catagacgta accgagctca cgcaatccgc cgaggagggc gcgggtggtt gggctccgcg
     1381 gctgggggcc cgccatatcc gaggtcagcg aagaggtgct caggattccg atccggtaga
     1441 ccttgctcgc cggctacgcg gcggcgaatt ggagcacgcc gagggtgccc agggtgagag
     1501 tgccgagtaa agcgcgccgg tccatcagta gatcactccc cgttcagcct agctcgcggg
     1561 ccctggttcg ttgcagcggg acattcaatg cctcgccgag atgcacggac tactcaatga
     1621 cttgatccgc ccgcaatagc agcgagtggg ggatcttgag gccgagggcc gtggctgtct
     1681 tggcgttgat caccaactcg aagctagtcg gctgctccac cggaagggtg gccggcgccg
     1741 cgcctttgag aatcttgtcg acgaaatagg cagcgcgtcg atactggccg acctggttga
     1801 cgccatacga catgaggcct ccgaaagcca cgaactcccg cgtccatgac acagcaggca
     1861 gtttagaccg cttcgccaaa tcggcgatgc ggcgcagatt ccggaaatgg atcaccgagc
     1921 ccagcatcgt gagaccgccg gcccgctctc gcttcgccgc gacgagcgcg ccatccagat
     1981 cgtcagccgt tcgtacggca agcggctgga gttcgaggcc gagtgaggcc gcgcccgttt
     2041 cgagcgtctt caaaaggtgt tcgtgagccg gttggggagg attccagagc acagcgacgc
     2101 gtttgatacc gggcgagacg gtccgaagca gctcgagcca cttcggcgcg agatcgtcga
     2161 cgacatttga gaggcccgtg atgttgccgc ctggtttcgc aaagctcgcg atgaatccct
     2221 gtccgacagc atcgccgacc atcgtcatga cgatcgggat ggaagccgtg gctcgtcgcg
     2281 cagtctgcac ggactcggag ccgccggcca cgatgacatc gacacgctgc cgcatcaagt
     2341 gagcgaccgc tgcgtcgagc cggtcgaatc taccctccac gtcctggtgc aggatcacca
     2401 catccgttcc ttcacgataa ccgaggtcgc gaagcccttg gcgcaacgct tcgacgttgg
     2461 ccgatccggt gaggacaccg acccgatgca gcttccccgc cggctgcgcc tcggtgacaa
     2521 gcggtgcggc gaggatgacc agggccgaaa gcaggaggag agcggcgctg gagcgtgacc
     2581 tcatgctgcc catgacgtgt atgcgcctcc cgtgtggctg cgaccgctga acctcgcacg
     2641 cgtcatcacg ccgctgtagc gaagggctga tccactagta gtcgagcctg ctgttgggcc
     2701 gccgctcatc agtcaatgac gcgatctgcg cgcagcagta gcggctgtgg gatcgtgagg
     2761 cccagcgcgg tcgcggtctt gcggttgatc accagttcga acttcgtcgg ttgctcgatg
     2821 ggcaactcgc tgggtttggc gcctctcagg atcttatcga cgtaggttgc agcacgacgc
     2881 ggcgcatcgg caacactggg gctgtaactc atgaggccgc cggcttgggg atagcgctga
     2941 gaggcataga ccgccggcaa gcgatgcttg agcgagagct cggcgatcag ggccgcgctg
     3001 gggagaaggc gaggacagtc gaaccccaga actgcctgga ctcgctgatt cgccgctgag
     3061 gcaaatgctc tcacgaggtc ttcgcgcccg ctcgctgcga ggggcgacaa ctgcacgccc
     3121 aggacatcgg cggcagcccg agtttctatc cattcttgct cggtcacagg cgtgccccgc
     3181 ctgcacccga gaacgcccac gcgcgacagc tttggcaaca gctcccgaag aagctccagg
     3241 cgcttgcgac tgagttccgg agagatcatg gtgaacccgg taacgtttcc gcccgggcga
     3301 gcgaggctca cggccagccc ctgcctgacc gcatctccgc tgcccaccat cacgatgggg
     3361 attgattgcg tggccttctt ggcagccacg gctgcggccc cggttgcagt gacgatgagg
     3421 tctatcttcc gacggacgag ctcggcggcc aggtccggaa gtcgttcagg ccttccttct
     3481 gcgtatcgga tctcaaagac gatgtttcgg ccttcgatgt agccgaggtc acgcaggccc
     3541 tgtcgcaagg aatcgagacg ctcgccatcg ggccccgaag acatggcgag atacccgagc
     3601 cggtagactc tctccgcccg ctggccgtcg gcggcgcgcg gagcgccgag aagcacgagg
     3661 ctcagagcga acagcaggca ggcgcctccg aacctcagag ccatttgttc gcagcctatc
     3721 cggctgcgta taccccgcga ggccgcgcgg cgagtcatgg gccaaacatc cggtcagccg
     3781 gattgcgccc gcgggccgga cccggcctgc ttggtcaggc gggccgccgg agacaggtcg
     3841 gccccaaccc gggtacggcg gctcagccag ccgccaccct cgttacaaga accggcctcc
     3901 ggccggttgt cgtgccgaca ctgcatgccc tgcgcctcct ggcgtccagc ggtcacacgg
     3961 tgcggtgagc ctagcccctg gggtctcgat ggtcaacgca gcccacaggc gtcggagacc
     4021 tacgagcacc cacccacggt gccggagagc gagctcttgg ggccccttcc ccaaccgcgc
     4081 ggtggaaggg gggcggccgc cggtcttttg ctgacgccac gctgacggct ttgccgggat
     4141 gcggtg
//
LOCUS       DASRJQ010000133         2921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3014416, whole genome shotgun sequence.
ACCESSION   DASRJQ010000133 DASRJQ010000000
VERSION     DASRJQ010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3014416"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1463
                     /locus_tag="VFB89_03105"
     CDS             <1..1463
                     /locus_tag="VFB89_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZM26316.1"
                     /translation="VGLATLCAGIALAGPRGGRAKVTAETHALNLVIAMDISRSMLAE
                     DVSPNRLQHAVRESRRLVQDIEGDRVGLIAFAGRSYILTPLTIDGGAVRMYLDGLDPD
                     LASEGGTSLAAVLQQGGQLLAVGGDAADRVLVVFTDGEGHDSLPDAVLQAQALKSRGV
                     HLVLVAEGRADPVKIPLRDSTGSLVEYKLDEQGQPVRTQRRDDILRALADAAEGTVVP
                     ADLPDQAGAVKNIVAAFKRSPISETRIADLVPRGWMPALAGVLVLLVFTFATRGAALA
                     VLAGVSVLAGTAQAQRPASGDRALAAGDPLGAAKAWLQQAGHGTARDTAFYNAGTAAL
                     EAGNFDVARGALTEAAKSADPALRYRALYNLGTAALEQARRDSTRRNALLDEATTNLR
                     SALVLQPGSARAKWNLELALRRRAPEAGGGGGGRPPSGGGGGQRPQPAEQAPGPQGLS
                     EAEAEQILNSMERQERATRSEQESRFRGGAGGVKDW"
     gene            1460..>2921
                     /locus_tag="VFB89_03110"
     CDS             1460..>2921
                     /locus_tag="VFB89_03110"
                     /inference="COORDINATES: protein motif:HMM:NF024973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HZM26317.1"
                     /translation="MTWLLAPVAWLWLQGGGLEVRLYTDEDRITLGDNLVLTVQATAA
                     GNLPVQLSLPNITGFEITGRSEHSEVSQLGTPSRTTTLELRLRALKPGRWELGPARAQ
                     QGTAVARAGPVIVQVDEVPGAATVASNPVLKRLLDRAPTPPHAGQPGVSLVLSSDSAA
                     VGEQVDVLTAAWFPRELRSQLRRPPTLQPPVIEGVWSYPQPAPPGIAATRSVAGVLYD
                     LFVAHQIVFPLQPGRIQVTPAILKYSVPIALQFFSQEERYTLQSAPAVLTVSPTPSAG
                     RPSEYAGAVGSGLRLERVVDPPSARAGEPVAVTFRLTGDGNPALWPAPEISWPSGVRA
                     YPDRTDEHLATSSGRLGGTKTFRYTVVPDSSGPLALPAATYTYFDVDARAFHSSVLAA
                     DRLIVAQGPEATASRPLPPPLLEPRGVELAHSITTDMPAWLWALIAVTPPVLFFGRRW
                     VRRRRRQRPAPVAADPRSAGFELENLIRELVPSPEAL"
BASE COUNT          478 a          964 c          980 g          499 t
ORIGIN      
        1 ccgtcggact cgctactctt tgcgcgggca ttgcgcttgc cggtccgagg ggtggacgtg
       61 ccaaggttac ggccgaaacc cacgcgctca atctggtgat cgcgatggac atttcgcggt
      121 ctatgctggc ggaagacgtg agcccgaacc gacttcagca tgccgtcaga gagagccgcc
      181 gcctggtaca ggacatcgaa ggcgatcgag tcggactgat cgcgtttgcc gggcggagct
      241 acatcctgac gccgctcacg atcgatggtg gcgctgtacg catgtaccta gacggactcg
      301 atccggacct ggccagcgaa ggaggtacca gtctggcagc ggtccttcag cagggaggac
      361 agctactggc ggtgggcggc gatgctgcgg atcgagtgct tgttgtgttc accgacggcg
      421 aggggcacga ttcgcttccg gatgccgtgc ttcaagccca agcactcaag tcgcgcgggg
      481 tgcacctggt gctcgtcgca gagggccgag cagatccggt aaagatcccg cttcgcgatt
      541 ccactggcag cctggtcgag tacaagctcg atgagcaagg ccaaccagtg cggacgcagc
      601 ggcgggacga cattctgcgg gctctcgccg atgcggccga agggaccgta gtcccggccg
      661 accttcccga tcaggctggg gccgtgaaga acatcgtcgc agcgttcaag cgaagcccga
      721 tctccgagac tcgcattgcc gacctggttc ccagaggttg gatgcccgcg cttgcggggg
      781 tgcttgtcct gctggtcttt acgttcgcca cgcgtggagc agcgctcgcg gtcctggcgg
      841 gcgtgtcggt cctggccggg acagcgcagg cccaacgccc cgcgtccggt gatcgtgccc
      901 tcgcggcggg agatccgctc ggcgcggcca aagcatggct tcagcaggcg ggtcatggga
      961 cggcccgaga taccgccttc tataacgcag ggaccgcggc cctcgaggcg ggtaactttg
     1021 acgttgcgcg cggggccctc accgaggcag caaagtccgc ggaccccgca cttcggtacc
     1081 gtgcgctcta caacctgggc accgcggcgt tagagcaggc ccgtcgcgat tccacccggc
     1141 gcaacgctct cctggatgaa gcgaccacca acctccgcag tgcactcgtg cttcagcccg
     1201 gctccgcccg tgctaagtgg aacctcgagc tggcattgcg ccgccgggct ccggaggcgg
     1261 gtggcggcgg aggtggacga cccccatccg gtggcggagg cgggcaacgt cctcagcccg
     1321 ccgagcaggc tcccggtcct caaggcttga gtgaggcgga ggccgagcaa attctcaact
     1381 cgatggaacg ccaggaacgg gcgacccggt cggagcagga gagccggttc cgaggtggcg
     1441 ccggaggagt caaggattgg tgacgtggct cttggcgccg gttgcgtggc tctggcttca
     1501 gggtgggggc ctcgaggtgc ggctctacac cgacgaggac cgcattacac tcggcgacaa
     1561 cctggtgctc acggtgcagg caaccgcggc cgggaacttg ccggttcagc tctcgcttcc
     1621 caacatcacc ggcttcgaga tcaccggtcg gagcgagcac agtgaagttt cacagctcgg
     1681 cactcccagt cgcaccacga cgctcgagct gaggctccgc gccctgaagc cgggccgatg
     1741 ggagctcggt ccggcccgag cacaacaggg aaccgcggtc gctcgagctg gacccgtcat
     1801 cgtacaagtc gacgaagttc cgggggccgc taccgtggcc agcaatccag tgctcaagcg
     1861 gctgctcgat cgcgcaccca ctcctccgca cgcgggccag cccggggtga gcctggtgct
     1921 ctcgagcgac agcgcagcgg tcggcgagca agtggatgtc ctgacggctg catggttccc
     1981 ccgcgagttg cgaagccaat tgcggcggcc gccgacgctc caaccgccgg taatcgaggg
     2041 agtgtggagc tatccgcagc ccgcccctcc aggcatagct gccacccgat cggtcgcggg
     2101 cgtgttgtac gacctgttcg tcgcccatca gatcgtcttc ccgctccagc ccgggcggat
     2161 tcaggtcaca ccggccatcc tgaaatacag cgtacccatt gcgctccagt tcttcagtca
     2221 ggaagagcga tacacgctgc agagcgcgcc ggcagtgctc actgtttcgc ccacgcccag
     2281 tgcgggccgc cccagcgagt atgccggcgc agttgggagc gggcttcgct tggagcgggt
     2341 ggtggacccg ccctccgctc gcgccggcga gccggtcgcc gtcacctttc gtctcacggg
     2401 tgacgggaac ccggcgctct ggccggcgcc cgagatcagc tggccttcgg gtgtgcgcgc
     2461 atatccggat cgaaccgacg agcatctggc aaccagctcg ggccgactgg gtgggaccaa
     2521 gacattccgc tatacggtgg tgccggattc atccgggccg ctggctctgc ctgctgcgac
     2581 ttacacctat ttcgacgttg acgcccgcgc atttcattcg agcgttctgg cggctgatcg
     2641 gttgattgtg gcgcaaggcc cggaagccac cgcctcacgc ccccttccac ccccgttgct
     2701 cgagcctcgg ggtgtcgaac tggcgcacag cattacgacg gacatgccgg catggctttg
     2761 ggcactgatt gccgtgacgc caccggttct gttcttcggg cgacgttggg ttcgccgtcg
     2821 tcgacgccaa aggccagcac cggttgccgc cgatcctcga agcgccggat tcgagctcga
     2881 gaacctgatt cgtgaactgg tgccgtcgcc cgaagccctg a
//
LOCUS       DASRJQ010000134         2459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3013264, whole genome shotgun sequence.
ACCESSION   DASRJQ010000134 DASRJQ010000000
VERSION     DASRJQ010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3013264"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..>2459
                     /gene="clpB"
                     /locus_tag="VFB89_03115"
     CDS             135..>2459
                     /gene="clpB"
                     /locus_tag="VFB89_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HZM26318.1"
                     /translation="MIRPERLTIKGQEAFRDAGEEARRRGNPVVNDAHLFAALLAQDD
                     GVVQPLLQKAGLNVTQLREAVEREIARFPTQSGGAEPTFSRELNRVFDRADNEAKTLG
                     DAYISTEHLLLALMDEKGTAARALLSARDISPADLRAALEGVRGSHRVTDQAPESQYQ
                     ALERYTRNLTDQARKGKLDPVIGRDEEIRRVMQVLSRRTKNNPVLIGEPGVGKTAIVE
                     GLAQRIVNGDVPDSLRDKELVALDIGQLLAGAKYRGEFEERLKAVVKELTSTEGRYIT
                     FIDELHTIVGAGAAEGAVDASNMLKPPLARGELHVVGATTLDEYRKHIEKDPALERRF
                     QPVLVGEPSVTDTIAILRGLKEKYEVHHGVRITDNALVAAATLSDRYIGDRFLPDKAI
                     DLVDEAASRLRIEIDSLPQEIDEVERRVLQLEIERQALLKEKDKAAVERRKALEQQIA
                     ELKEQSAGMKAQWQAEKAAIQEIQARKAEVEQLRNEAEQATRRGDLQRAAELRYGRIP
                     ELERELEARDKRLQEVQAKAKYLKEEVDAEDIAEIVAKWTGIPVSKMLESERERLTKL
                     ESELARRVVGQETAVTAVANAVRRSRAGLQDPGRPTGSFIFLGPTGVGKTETARALAE
                     FLFDDERALVRLDMSEYMEKHSVARMIGAPPGYVGYEEGGQLTEAIRRRPYSVVLFDE
                     IEKAHPDVFNVLLQILDDGRLTDSQGRVVDFRNTVIIMTSNVGSQLIVDAGAQPSDQA
                     WARLEQRVRDELRNYFRPEFLNRVDDVIVFHQLSR"
BASE COUNT          490 a          742 c          818 g          409 t
ORIGIN      
        1 ttctcgcggt ctgtcttttt ggcagttatt ccggtacacc ccttgccaac gcttaacccg
       61 aatccaatcc tagctctcct ggcggtggaa attcgctggg agtcatacgt tgtaagcgat
      121 cgaggtggta accaatgatt cgacccgaac gcctgaccat taaagggcag gaagcgtttc
      181 gcgatgccgg cgaggaggcc aggcgccgcg gtaatccggt cgttaacgat gcgcatctct
      241 tcgcggccct gttggcccag gatgacggcg tcgtacagcc gctcctgcag aaggccgggc
      301 tcaacgtcac ccaactgcga gaggcggtcg agcgcgagat cgcccgattc cccactcaat
      361 ccggcggcgc cgagcccacg ttcagccgag agttgaaccg cgtattcgat cgggccgaca
      421 acgaggcaaa gaccctcggt gatgcctaca tctcgaccga gcatcttctg ctcgccctga
      481 tggacgagaa ggggactgcc gctcgcgccc tgctcagtgc tcgggacatt tcccctgccg
      541 acctgcgtgc agcgctcgaa ggagtccgcg gatcgcaccg cgtgaccgac caggcacccg
      601 aatcccagta ccaggctctc gagcgctaca cccgcaacct cacggatcag gcccgcaagg
      661 gaaaacttga cccggtcatt ggtcgggacg aagagattcg ccgcgtgatg caggtgctgt
      721 cccgtcggac caagaacaac ccggtgctga tcggcgagcc gggcgttggc aagacggcga
      781 tcgtcgaggg actcgctcag cgcatcgtca acggcgacgt ccccgactcg ctgcgtgaca
      841 aggagctggt tgccctggat atcggccagt tgctcgcggg cgccaagtat cgcggcgagt
      901 tcgaagagcg tctgaaagcg gtggtcaagg agctgacctc gaccgaaggg cgctacatca
      961 ccttcatcga cgagctgcac accatcgtag gcgcgggcgc cgccgagggc gccgtcgatg
     1021 ccagcaacat gctcaagccg cctcttgccc ggggcgagct gcacgtggtc ggtgccacca
     1081 cgctcgatga gtaccgcaag cacattgaga aagaccctgc gctcgagcgg cgattccagc
     1141 ccgtcctcgt gggcgagcct agcgtgaccg acacgatcgc gatcctgcgg ggactcaagg
     1201 aaaagtacga agtccaccac ggggtccgga tcactgacaa tgccctggtg gcggcggcaa
     1261 ccctgtccga ccgatacatc ggcgatcgct tcctgcccga caaggcgatc gacttggttg
     1321 acgaggctgc gagtcgcctt cgtatcgaga tcgacagcct tccccaggag atcgacgagg
     1381 tcgaacgccg ggttctgcag ctcgagatcg agcggcaggc gttgctcaag gagaaagaca
     1441 aggctgcggt cgagcgccgt aaggctctcg agcagcagat cgccgagctc aaggagcaga
     1501 gcgccggcat gaaggcgcaa tggcaggcgg aaaaggccgc gatccaggag atccaggcac
     1561 gcaaggcgga ggtcgagcag ctccggaacg aggcggagca ggcgactcgg cgcggcgacc
     1621 ttcagcgtgc cgcggaattg cggtacggca ggattccgga gctcgagcgg gagctggagg
     1681 cgcgcgacaa gcggctccag gaggtccagg caaaagcaaa gtatttgaag gaagaggtgg
     1741 atgcggagga tatcgccgag atcgtcgcca agtggaccgg gatcccggtg tcgaagatgc
     1801 tcgagtctga gcgtgagcgg ctgaccaagc tggagagcga gctggcccgt cgggtggtgg
     1861 ggcaagaaac cgcggtaacg gcggtggcga atgccgtgcg acggagccgc gcggggcttc
     1921 aggaccccgg ccggccgact gggtccttca tcttcctggg tcctaccggc gtcggcaaga
     1981 ccgagaccgc ccgcgcactc gccgaatttc ttttcgacga tgagcgcgcg ctggttcgct
     2041 tggacatgtc ggaatacatg gagaagcatt cggttgcccg tatgatcggt gcgcccccgg
     2101 ggtacgtcgg ttatgaggaa ggcggccagc tgacagaagc gatccgccgc cggccgtata
     2161 gcgtcgtgct gttcgatgag atcgaaaagg cgcacccgga tgtgttcaac gtcctgcttc
     2221 agattctgga cgatggacgg ctcaccgaca gccagggtcg tgtggtggac ttccggaaca
     2281 cggtcatcat catgacgtcg aatgtgggca gccagctcat cgtggatgcg ggcgcgcagc
     2341 ccagtgatca ggcttgggct cggttggagc aacgggtccg cgacgagctg cgaaactact
     2401 tccgtccgga gttcctcaat cgagtggatg acgtcatcgt gttccaccag ctcagccgg
//
LOCUS       DASRJQ010000135         2232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_234954, whole genome shotgun sequence.
ACCESSION   DASRJQ010000135 DASRJQ010000000
VERSION     DASRJQ010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234954"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VFB89_03120"
     CDS             complement(<1..342)
                     /locus_tag="VFB89_03120"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HZM26319.1"
                     /translation="MHVLWNVDPVMVHLGPIELRWYGLLFAGGILAAYLVGVWSFEQA
                     GGSREETNRLLGYVVIGTIVGARLGHCLLYEPGYYLRHPLEIVAVWRGGLASHGGVAG
                     IVVAVWLFGRRT"
     gene            complement(1092..>2232)
                     /locus_tag="VFB89_03125"
     CDS             complement(1092..>2232)
                     /locus_tag="VFB89_03125"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZM26320.1"
                     /translation="MNLQDRGINNFGKFAATAQEFAQDLGKTGAVVGVNTTIRADAQQ
                     LYLDVDRDKVKSLDLSLDDVFTTLQTMLAYDYVNDFNLYGKTYRVQVEGQASFRQRPE
                     DLGKFYVRSATGTMVPLGALVRTSFRAGPTVVNRFNGFTSALVIGAPGPGKSSGQMLE
                     AAEKLIKDKYEAMNVGYGYSGESFQESESSGQGGVVFGLGLVMAFLVLAAQYESWSTP
                     LAVMIGVPFGVLGAFLGLLVLRHPHDLYFDIGLLVVIGLEAKNAILMVEFAIEQRAAG
                     RSIEDAAVEAGRERIRPILMTSFAFILGVAPLIVASGAGAAARHSLGIGVFFGMLVST
                     LLGVNIIPNLFIFVRKLSERMAARRSGSPARSPARDVSPRPAGDD"
BASE COUNT          441 a          751 c          662 g          378 t
ORIGIN      
        1 ggtgcggcga ccaaacagcc agactgcgac cacgatgccg gcgacgccgc cgtggctcgc
       61 gaggccaccc cgccagaccg ccacgatctc gagcggatgt ctgaggtagt accccggctc
      121 gtacaagaga cagtgaccta gacgggcacc gacgatcgtt ccgattacca cgtaacccag
      181 taagcgattg gtctcctccc gcgagcctcc ggcctgctcg aagctccaca ccccgaccaa
      241 gtaggctgcg aggatgccgc cagcgaagag caagccatac cagcgaagct cgatagggcc
      301 gaggtgtacc atcaccggat cgacgttcca caggacatgc atcagcgact ccttcagcgg
      361 accctcgcaa cgccgaaccg cggcacaaca ttggcgctca cagccttccc gttagggtct
      421 gtaaaggcca ctcccaccgc ggggtcggag gagttctgaa tcacacgcag atgtcgtccg
      481 gaacctgggt gcaatgtgcc cgagtgatca taagtggcgg ctttcattaa actgtccggt
      541 tggagcgggt agctgccgaa gttcgtggta aacaccgacc gcaagttcca gctcttatat
      601 cgtagccgtg cggtcggctc gatgaacccg ggtatcgccg tgagatagtt agccatctgc
      661 cacgccccaa ccaggggttg ggaaatcgac cccaacgacg ctgcttacgt tgatgctccc
      721 cgtcaggccc gttccgcttg ggctgtccgc gccagctcgg ggcgagcggg agcccgacgc
      781 ccggactcac ggtgagcggc agctcgctcc atctccctat tgggctggcc ttcgatccgc
      841 cgccggcgaa tctgccgctg ccgtagggcg gcggccgggg cgcttaacag tggctccagc
      901 tgatcaactt tctcgggctc gcccacgatc acgcaggagt cagcgggact cagagacgcc
      961 tgccagcgcg tgcgcctgcc gtgtcgccgg atgatccgcg ctcgcgggct gaacgactga
     1021 cgcggaggga agtatctacc gtgacgatag cccgtggatc attctcgaaa cgtggtagct
     1081 aatccgtcac ctcagtcgtc tccagcgggt ctgggcgaca cgtcccgtgc tggcgagcga
     1141 gccggcgagc cggagcgacg cgccgccatg cgctcactca acttccgtac gaagatgaac
     1201 aggttcggga tgatgttgac gcccagcaac gtcgacacga gcatcccaaa gaacacgccg
     1261 atgccgaggg aatgccgagc cgccgccccg gcgcccgagg cgacgatgag cggggcgacg
     1321 cctagaatga acgcaaagga cgtcatcagg atcggtcgaa tccgctccct ccccgcctcc
     1381 accgcggcgt cctcgatgga tctgccggcg gctcgctgtt cgatggcgaa ctcgaccatc
     1441 aagatggcgt tcttggcctc gagcccgatc acgacgagca acccgatatc gaaatacaga
     1501 tcgtggggat gacgcagaac caacaatccg agaaacgcgc cgaggacgcc gaagggtaca
     1561 ccgatcatca ccgcgagcgg cgtcgaccag ctctcgtact gtgcggccag aaccagaaac
     1621 gccatcacca ggccgaggcc gaacacgacg ccgccctgcc cgctcgactc cgattcctga
     1681 aaggactcac cgctgtatcc gtatccgacg ttcatcgcct cgtacttgtc cttgatcagc
     1741 ttttccgcgg cctccagcat ctgccccgag ctcttgcccg gtcccggggc tccaatgacc
     1801 agcgccgacg taaacccgtt gaatcggttc accacggtgg ggcccgcccg gaatgacgtg
     1861 cgcaccagag caccgagggg caccatcgtg ccggtggcgc tccgcacata gaacttgccg
     1921 aggtcttccg ggcgctgccg gaacgacgcc tgcccctcca cctgcacacg ataagtcttg
     1981 ccgtacaggt tgaaatcgtt cacgtagtcg taggccagca tcgtctggag agtcgtaaag
     2041 acgtcgtcca gcgacaggtc cagcgatttc accttgtcgc gatcgacgtc gaggtacaac
     2101 tgctgcgcgt ccgcccggat ggtggtgttg acgccgacca cagcgcctgt cttccctaga
     2161 tcctgagcga actcctgggc cgtcgcggcg aacttcccga aattattgat gccgcggtcc
     2221 tgcagattca tc
//
LOCUS       DASRJQ010000136         3716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2445597, whole genome shotgun sequence.
ACCESSION   DASRJQ010000136 DASRJQ010000000
VERSION     DASRJQ010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2445597"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VFB89_03130"
     CDS             complement(<1..360)
                     /locus_tag="VFB89_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26321.1"
                     /translation="MRGNTWLVGIGVVLAVAFILVILIWLLWPSLVEGNLVFTTRKMC
                     QLTGEWDKQFNQQTRNFDRGFGITGSDLGFPVRWNDRLIFLFGDTRSTDPEVFIPPGQ
                     DRFDSIADAPLNADVDRE"
     gene            829..1341
                     /locus_tag="VFB89_03135"
     CDS             829..1341
                     /locus_tag="VFB89_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26322.1"
                     /translation="MSLIHIGTLLLGAVVGAALALVIPAAMERSRYAARRDLLGSWFS
                     SYQQYQGEASKGWVEEKIDIRLEGAKFLLESSDNSIGEYCRATATLHAGELLGKWASI
                     RHEGGNAYGGMLLTVLPEEGVIYGIFSGPRDSGQRIHGAWVMARKEEDLAKAKRLVGA
                     QILEMNDQRR"
     gene            1344..1577
                     /locus_tag="VFB89_03140"
     CDS             1344..1577
                     /locus_tag="VFB89_03140"
                     /inference="COORDINATES: protein motif:HMM:NF040329.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6116 family protein"
                     /protein_id="HZM26323.1"
                     /translation="MPDQGLLSRFIGRLRFPQLFFLAAILLGIDLVVPDAIPFLDEAL
                     LALLTAMLAMLRRRDPQPDPGQPGRIEKNVTPR"
     gene            complement(1564..1740)
                     /locus_tag="VFB89_03145"
     CDS             complement(1564..1740)
                     /locus_tag="VFB89_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26324.1"
                     /translation="MAFTADQANAIVARTLASVTVAIAYHGTDDETDTTIDVDAVVGP
                     WRIVRGGRADASVG"
     gene            complement(1940..2908)
                     /locus_tag="VFB89_03150"
     CDS             complement(1940..2908)
                     /locus_tag="VFB89_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26325.1"
                     /translation="MAGIETLTVDATGKVTATLPSSTSSFDLSAVVSDAQALNSAINS
                     NTTMVITAFGGAGAAGQNATASGTGEKESGGTAGAGGSAQMVTTLADLSSTYGTTKIY
                     YYLGSQGSDEHAGGKGGSSTIVATADLTSTMAATSCVLLIAGGGGGGGAAFNTSGGSG
                     GSGGKAVSQIGAAATAAGANGGSHGGSGGSNGTGGAGGQAGGGSGSIDGTAGNSGLGG
                     QGGPVHVGSGPSTSTGWLNVSSVPGGIGTNGQGGEGEWRSDSAGEGGGGGGGYGGGGG
                     GAAGGGSNNGGGGAGGGSYAAASTATASLSLTSASDDGSVIITLIP"
     gene            3294..3605
                     /locus_tag="VFB89_03155"
     CDS             3294..3605
                     /locus_tag="VFB89_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26326.1"
                     /translation="MARKIRYTPEGGALFEITCRTIHSRFLFRPTRRFNEILVGILAR
                     AKTRYGVEISAFCCRHSGDPGARSAALLEGPAPRTVPSPHCRPRRGHHRNGSGAPEAI
                     G"
BASE COUNT          653 a         1294 c         1107 g          662 t
ORIGIN      
        1 ttcgcgatcg acatccgcat tgagcggagc atcggcgatg ctgtcgaacc tgtcctgtcc
       61 cggtgggatg aacacctcgg ggtcggtgct ccgcgtgtca ccgaacagga agatgagcct
      121 gtcgttccag cgaacgggaa aacccagatc ggatcccgtg ataccgaagc ctcgatcgaa
      181 attgcgcgtc tgctgattga actgcttgtc ccactcacct gtgagttgac acatctttcg
      241 tgtcgtgaag accaaattgc cttcaaccag cgatggccat aagagccaga tcagaatgac
      301 cagaatgaag gcgactgcca acaccactcc aataccgact agccaagtgt ttccccgcat
      361 tgcactaacc ctcggatctc ggccaacgct gctgcggctc agcgcgcggc gccagccgtc
      421 cgctggagcc gcttggttgg ccgaaccaag gttgcgtgct cgcgtatatg gctatggctc
      481 caccgcgctg ccttgtttaa ttatacaacg gacctgccgg ccggcgaagc gacctgcctc
      541 cgagcccacg accggttcgt ctggccacca aggccccgct tggctccacg aggtttccag
      601 tcgcctcggt cggtccatcg atcggcaagg tggtagagag agtgcacagg gattccgcag
      661 accgggcagt ttggccgtag cgaagcggag gccacggaac cgagactcct gcgcggtgta
      721 aacggtctag aataagagta ggcaccgttg ggtggctcct gcggctgccc tacaggatac
      781 tgccccgcga agccgtcgat tcgtgaccca ctccggggag gagatgaaat gagcttgatc
      841 catatcggca ccttgctgct cggcgcggtg gtcggagcgg ccctggcact cgtgatcccc
      901 gcggcgatgg agcgcagccg ttacgccgcg cgtcgcgacc tgctcggctc gtggttctcg
      961 agctaccagc agtaccaggg agaggcgtcc aaggggtggg tcgaggagaa gatcgacatc
     1021 cgcctcgagg gggccaaatt cctcctcgaa agctccgaca actcgatcgg cgagtactgc
     1081 cgggccaccg caacccttca cgccggagag ctcctcggta agtgggcctc gatccggcac
     1141 gagggcggga acgcgtatgg cgggatgctg ctcacggtac tgccggagga aggcgtgatc
     1201 tacggcatct tcagcggacc tcgcgactcg ggccagcgca tccatggcgc ctgggtgatg
     1261 gcgcggaagg aggaggacct cgccaaggcg aagcgcctgg tcggagccca gatcctcgag
     1321 atgaacgacc agcggagata accatgccgg atcaaggcct cttgtcgcgg ttcatcgggc
     1381 gcctgcgctt cccccaactc ttcttcctcg ccgccatcct cctggggatc gacttggtcg
     1441 tcccggacgc catcccgttc ctcgacgagg ccctgctcgc cctccttacg gccatgttgg
     1501 ccatgctgcg gcgccgtgat ccgcaacccg acccggggca gccggggcgg atcgagaaga
     1561 acgtcacccc acgctagcgt cggcacgacc gccgcgcacg atgcgccatg gtcccacgac
     1621 ggcgtcgacg tcgatcgtcg tgtccgtctc gtcgtcggtg ccgtggtagg cgatggccac
     1681 cgtcacagaa gcgagggtcc tggctacgat ggcgttggcc tggtccgcgg tgaacgccac
     1741 agcccagtct ttcccccgag gcggagctct acgccactga tccgcaagcc atccagacgg
     1801 cgtttggccg ggctgcacag cgacgtcttc tcgggagtga tcgacccggc caccggcatg
     1861 gccgggtctc ggaacctggg ctaccgcatc gccttcgcgg acgcgatcga gctgccatag
     1921 tcagatctcg tcatctgagt caagggatca gcgtgatgat cacagacccg tcgtcgctcg
     1981 cgctcgttag gctgaggctg gcggtcgcgg tcgatgcggc cgcgtagctt ccgccgccgg
     2041 caccaccacc accattatta gacccgcccc cagctgcgcc accgccccca ccaccgtatc
     2101 caccaccacc accaccgcct tctccggcac tgtctgagcg ccattcacct tcgccgcctt
     2161 ggccgttcgt gccaatcccc ccgggcacgc tgctcacgtt caaccagccc gtcgacgtcg
     2221 agggcccgct cccgacgtgc accggcccac cctggccacc caggccgctg ttgccagccg
     2281 ttccgtctat agaaccggag ccgcctcccg cctggccgcc ggctccgccg gtgccgttgg
     2341 agccgccaga cccaccgtgc gagccgccgt tggcacccgc cgcggtggcc gcggccccga
     2401 tctgactgac ggcctttcca ccgctcccac ccgagccgcc actcgtattg aacgcggcgc
     2461 caccgccgcc accgccgccg gcgatgagca gcacgcagga agtcgcggcc attgtcgagg
     2521 taagatcggc tgtcgcgacg atcgttgatg agcccccttt gccgccggca tgctcgtcgg
     2581 agccctgcga accgaggtag taatagatct tggtcgtacc gtaggtggag ctgagatcgg
     2641 ccagcgtggt caccatctgc gcgcttccac ccgcgccggc cgtgcctcct gattcctttt
     2701 cgcctgtgcc gcttgcggtg gcgttttgtc ctgctgctcc agccccgccg aaggcggtga
     2761 tgaccatcgt agtattcgag ttgatcgctg aattgagagc ctgcgcgtcg ctgaccacgg
     2821 cgctcagatc aaacgatgac gtgctgctgg ggagagtggc cgtgaccttt cctgttgcat
     2881 cgaccgttaa agtctcgata cctgccatgg ttatcctcct cccacgaatc caacgcgaaa
     2941 ttgggtggaa acctcttttg atcgctgctc gttgctggaa cgctccgaag tctagagcac
     3001 cagggacctc ggtggcaagg gggtgcggag ccagcagcaa taggttccac ccgattctac
     3061 acccagccca gcgaggatca gaaacgccag ggattgcgcg gagcgccaga tccctggaca
     3121 cagctaaacc tcgacgattc cacaacttgc cggatggggc gcaagatccc tggacatcgc
     3181 ccggcctgcc gcttgcctgg agaaggccgg gatctccctt ccattcaagg acttgcctcg
     3241 cagccctcgc tatcccgtga gctggcagcc ctcttgcccc tggcgcctcc cccatggcaa
     3301 gaaagatccg ctataccccc gaaggcggcg ctctcttcga gatcacctgc cgcaccatcc
     3361 acagccgctt cctgttccgg cccacacgcc gattcaacga gatcctcgtc ggcatcctcg
     3421 ccagggccaa gacgcgctac ggtgtcgaga tctcggcctt ctgctgtcgc cactcgggag
     3481 atcctggagc tcgatccgct gccctgctgg aagggcctgc tccccgaaca gtaccgtcac
     3541 cacattgccg gcctcgtcga ggccatcatc gcaacggcag cggcgcaccg gaagcaatcg
     3601 ggtagagagc ccgcaggcgc ggctgcgatt cgatcacaga accccttgac ccaacccgcc
     3661 aggaccaagc ggtctcccgc tcctctcgtt catgccgcca gcaagcgcat gcgcga
//
LOCUS       DASRJQ010000137         2951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3851397, whole genome shotgun sequence.
ACCESSION   DASRJQ010000137 DASRJQ010000000
VERSION     DASRJQ010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3851397"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VFB89_03160"
     CDS             complement(<1..903)
                     /locus_tag="VFB89_03160"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZM26327.1"
                     /translation="MSSSKAQRWAVRGASRLALDRNTGAAAASMFLMALGEHLWRRFL
                     PKYLEALGAPVVAIGAYGSTEDFLDAIYQYPGGWVGDRYGRRRALTLFICVAAIGYGV
                     VALAPTWPVVLAGVVLVMAWSSMASPTLFAVIGDALPTRRRALGFSVQSILRRIPIAI
                     APTIGGVLIATYGVRDGVRFGLVISIVVACTAALVASRISLSLPRPIASLGVRDVWAT
                     VPGPLRRLLLSDIFIRTCEGMVDVFLVLYALNVVGISAPQFGLLIAIQMATAIACYLP
                     AAHFADRFGRKPFVIATFLAFAAFP"
     gene            complement(900..1319)
                     /locus_tag="VFB89_03165"
     CDS             complement(900..1319)
                     /locus_tag="VFB89_03165"
                     /inference="COORDINATES: protein motif:HMM:NF021357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HZM26328.1"
                     /translation="MLWVTRPHPHIDRTACAWLIRRFVDRDAAFGFAPDPDGARELGG
                     TPFDMRGVELGHHEGRCSFETILLKYGLVDPALHEIATMVHDADLDDDKFRSPEAPGL
                     DAVIRGLGMVIEDDNELLALTDRVYDGLYAWVQRMTR"
     gene            complement(1303..1842)
                     /locus_tag="VFB89_03170"
     CDS             complement(1303..1842)
                     /locus_tag="VFB89_03170"
                     /inference="COORDINATES: protein motif:HMM:NF042524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Chromate resistance protein ChrB"
                     /protein_id="HZM26329.1"
                     /translation="MALETWILLSSRLPREPTRLRLAVWRRLKRIGAVLLHDSVWILP
                     ADAKTREAFEWLAEEIEEQGGTALTWEAQSLSADQDRRLVQQFRSEADERYEELAASA
                     KAIRRAAIGRRRKLESGQLAQAIRQLRGLDRALRLERRRDYFRAPGRVAAEALLEEST
                     RELEAHLEPKEGARGALGH"
     gene            2013..>2951
                     /locus_tag="VFB89_03175"
     CDS             2013..>2951
                     /locus_tag="VFB89_03175"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZM26330.1"
                     /translation="MSPQDSSFSHLDRRSFLENAAAVTAAIALPGQVSFGRNRPPDLT
                     PIFAQIEKRHDEAVQRLQEWIRQPSIAAENKGVNEGCALTMRMLSDAGFDTVTKIPTD
                     GQPGIFATLDAGASRTVGLYFMYDVKQVDPAEWTSPPWEAALVDKQGLGKAVVGRGAV
                     NQKGPEATFLAALHAIRGAGRKPPVNLVLVAEGEEEIGSPHFPQIVRRPEVLAALRKA
                     MGIFMPSASQGLDGQVTINLGAKGVIECELVSTGERWGRGPQKDIHSSNKARVDSPAW
                     HLVQALSTLVSADGNEPAIDNFAAKVRPVSAGEKAMI"
BASE COUNT          593 a          953 c          940 g          465 t
ORIGIN      
        1 cggaaacgca gcaaacgcga gaaacgtggc gatgacaaat ggcttccggc caaaccggtc
       61 ggcgaagtga gcggccggga gatagcacgc gattgcggta gccatctgaa tggcgatgag
      121 gaggccgaac tgaggtgcgc tgatgccgac gacgttgagg gcatagagaa caagaaagac
      181 atccaccatt ccttcgcagg tccggatgaa gatgtccgac agcagcaacc gccgaagcgg
      241 gccgggaacc gttgcccaca cgtcgcgcac gcccagtgaa gcgattgggc ggggaaggct
      301 gagactgatg cgagacgcta ccaacgcggc tgtgcaagcc actacgatcg agatcacgag
      361 gccaaaccga acgccatcgc gcacaccgta cgtggcgatg agcacgccgc caatcgtggg
      421 ggcaatcgcg atgggtatcc gcctgaggat cgactgtacc gagaagccga gggcgcgtcg
      481 ccgcgtggga agcgcgtccc cgatcacggc gaagagcgtg gggcttgcca tgctcgacca
      541 cgccatgacg agcacgacgc ccgcgagtac gacgggccag gtaggggcga gggcaacgac
      601 accgtagcct atcgccgcga cacagatgaa gagcgtgagc gctcgcctcc ggccgtagcg
      661 atcgccgacc cagccgcccg ggtactgata aatcgcgtcg aggaaatctt ccgtgcttcc
      721 atatgcgccg atggcgacca ccggcgcgcc cagcgcctcg aggtacttcg gcaggaaccg
      781 tctccagaga tgttccccca gtgccatgag gaacatcgag gcagcagcag caccggtatt
      841 ccgatcgagg gccagtcgcg aggcgccccg caccgcccag cgctgcgcct tcgatgagct
      901 catcgggtca tacgctgcac ccaggcgtag agtccgtcgt agacccgatc cgtgagcgcc
      961 agaagctcat tatcgtcctc gatgaccatc ccaagcccac ggatcacggc gtcgagcccg
     1021 ggtgcctcag gagagcggaa cttgtcatcg tcgagatccg cgtcgtgcac catcgtcgcg
     1081 atctcgtgca gcgcaggatc aaccaggccg tacttgagca ggatcgtctc gaaggaacac
     1141 cggccttcat ggtggcccag ctcgacgccc cgcatgtcga acggcgttcc cccgagctcg
     1201 cgggcgccgt ccggatcagg cgcaaatcca aaggccgcgt cgcggtcgac aaatcgtcga
     1261 atcagccagg cacaggcggt gcgatcgatg tggggatgcg gcctagtgac ccaaagcacc
     1321 acgcgctcct tccttcggct cgagatgcgc ttccagttcc cgcgtcgatt cctccagcaa
     1381 tgcctcagca gcaacccgtc ccggcgcccg gaagtagtcc cgtcgtcgct cgaggcgaag
     1441 cgcccggtcg aggcctcgaa gttgtcgtat ggcctgggcg agttgccctg attccagctt
     1501 acggcgtcgc ccaatcgccg cgcgtcgaat ggctttggcc gaagctgcga gctcttcgta
     1561 acgctcgtct gcctcggacc ggaactgctg gaccaatcgc cgatcctggt cggccgagag
     1621 gctctgcgct tcccaggtga gcgcggtacc tccctgctcc tcgatttcct ctgccagcca
     1681 ttcgaaggct tcccgggtct tcgcatcggc cggtagaatc cacaccgagt cgtgcagcag
     1741 gaccgcgccg attcgtttga gacgtcgcca gacagcgaga cggagccggg ttggctctcg
     1801 cggcagacgc gacgacagga gtatccaggt ttccagcgcc attgagccaa tgtaacgatg
     1861 gttacattgc cggcaaggtc ggcggcgcca acaggccgat cgttctcgaa acgtcaggcg
     1921 caaaccaggc gccgccttga ccccgatgcc ccgggccgat aatggtcgtt cgaattgatc
     1981 acggccaact ggcacacgat tcggaggcgg tcatgagccc gcaggactcg tcgttctccc
     2041 acctcgatag acgttccttc ctggaaaatg cggccgcggt caccgcagcc attgcgctgc
     2101 ctggtcaggt ctcgttcgga cgtaaccgac ccccggatct gaccccgatc ttcgcgcaga
     2161 tagaaaaacg gcacgacgag gcggtacaac gccttcagga atggattcgg cagccatcga
     2221 tcgcggcgga aaacaaggga gtgaacgaag gctgtgcgtt gaccatgcgc atgctgtccg
     2281 acgccggctt cgacactgtg accaaaattc caaccgacgg ccaacctggc atcttcgcca
     2341 cactggatgc cggggcctcc cgcacggtcg gcctctactt catgtacgac gtcaagcagg
     2401 tcgacccggc cgagtggact tcgcctccct gggaggcggc gctggtcgat aaacagggtc
     2461 tgggaaaggc agtggtgggc cgaggagccg tgaaccagaa aggcccggag gccacgttcc
     2521 tggcggcgct gcacgccatt cgcggcgccg gcaggaagcc gccggtgaac ttggtcctcg
     2581 tcgccgaggg cgaggaagag atcggctcgc cgcactttcc gcaaatcgtc cgccggccgg
     2641 aggtgctcgc ggccctgcgc aaggcgatgg gcattttcat gccctccgcg agtcagggac
     2701 tcgacggaca agtgaccatc aatcttggcg ccaaaggcgt gatcgagtgc gagctggtct
     2761 cgaccggaga gcgctggggc cggggaccgc agaaagacat tcactccagc aacaaggccc
     2821 gcgtagacag cccggcgtgg catctggtcc aggcgctgag cacgctggtc tcagccgacg
     2881 gcaacgagcc cgccatcgac aactttgcag caaaggttcg cccggtctcg gccggcgaga
     2941 aggcgatgat t
//
LOCUS       DASRJQ010000138         2923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1355317, whole genome shotgun sequence.
ACCESSION   DASRJQ010000138 DASRJQ010000000
VERSION     DASRJQ010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1355317"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="VFB89_03180"
     CDS             <1..730
                     /locus_tag="VFB89_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HZM26331.1"
                     /translation="LRSYVDAVFEGRLTLPETRRLGFPWSEQLRERSLHTVQATIEAA
                     RDAMNSGAGATLAGGTHHAFADHGEGFCVFNDVAVAIRLLQREGRLRRAAIVDLDVHQ
                     GNGTARIFAGDADVFTFSMHGAGNFPFHKEESSLDIALPDGTGDSAFLASLTAHLEHV
                     IATARPELVFYLAGADPYREDRFGRLAMSVDGLRARDAHVFDCCRRHALPVVVTMAGG
                     YARAPADVVTIHTNTLTELRRVYG"
     gene            768..1577
                     /locus_tag="VFB89_03185"
     CDS             768..1577
                     /locus_tag="VFB89_03185"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZM26332.1"
                     /translation="MRGVSLFERRVGSGDPVAVLHGGPGADHGYLLPGFDLLAEGREL
                     IYYDQRGGGRSPVPRDTRVGWREHVADLDALRELWGLERLVIVGYSWGGLLAQLYATE
                     HPARVTRLALVSPGPSWRAARDEFERRFAQRNLAPELQAEREAVRASDLRVRDPEAFQ
                     RRMFELSVVPYFFDPGKARELTPFRVVGRTQEEVWKSLGNYDLRSRLPALRSTPSLIL
                     HGEDDPIPIEASRETANLLGAAFHSLDRCGHCPHVERPREFKEIVSGFLSG"
     gene            1632..>2923
                     /locus_tag="VFB89_03190"
     CDS             1632..>2923
                     /locus_tag="VFB89_03190"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase N-terminal
                     domain-containing protein"
                     /protein_id="HZM26333.1"
                     /translation="MPFRLMRFLCLLLPIAATPVLAQQDSVFAPLHAVDRANFDTTCA
                     PCRDFFGYVNGGWLARTTIPPQYSITGVDRDIQDRTEALLRRILDKAARDAKNATDAD
                     TRRIGLFYGSCMDSVRVDREGAKPLRSRLDRIAAIRSRAQLVGVMAELEREGVNGPIP
                     YLAYPNFKNSRVMAFHLYQGGLGLPDRDFYLRNDSQFTAIRREYTAHVGRMLALLDSP
                     KDSADADARRVVALETAIARTSIPAQDARAFSRLYHPTARASLLRMAPDIDWTGFFSA
                     TAVGAPDTINVAIPGFIRGVDSLIRTASIADWQAYLRWQLATATAPALSTPFQQEALV
                     LRRLTRGETQLKPRWQRCQQATDQAIGQALGREYVKAAFPPEAKARMQAMIANLRAAM
                     RDRLNTLTWMSDSTKGEAFKKLDAVSPKIGYPDKWRDYS"
BASE COUNT          487 a          947 c          961 g          528 t
ORIGIN      
        1 cttgcggagt tacgtggatg cggtattcga aggcaggttg actctgcccg agaccaggcg
       61 gttgggtttt ccctggagcg agcagttacg ggagcgctca ctgcacaccg tgcaggcaac
      121 catcgaggcc gcccgagacg cgatgaattc tggcgccggg gctaccctcg ccggcggcac
      181 ccatcatgcc ttcgccgatc acggcgaagg tttctgtgtg ttcaacgacg ttgcggtggc
      241 gatccgcctg ttgcagcggg agggccgact gcgccgggcg gcgattgtgg acctcgacgt
      301 gcaccagggc aacggaacgg cgcgaatctt cgccggcgac gccgacgtct tcaccttctc
      361 gatgcatggc gccggcaact ttccgtttca taaggaagag agctcgctcg atattgcgct
      421 tccggatgga accggcgact cggcattcct ggcgtcgctc acggcgcatc tcgagcacgt
      481 aattgccacg gcgcggcccg agttggtgtt ctatctggct ggtgccgacc cctatcgcga
      541 ggatcggttc ggccgactcg cgatgtcggt cgacggcttg cgggcgcgcg atgcgcacgt
      601 cttcgattgc tgccggcggc acgcgctccc agtcgtggtt acgatggcag gggggtacgc
      661 ccgggcgccg gcagatgtgg tcacgatcca caccaacacc ctcaccgagc tgcggagggt
      721 gtacggctga cgccactgag attgtgccgc gcaccgcaat gcgccaagtg cggggcgttt
      781 ctctcttcga gcgccgggtg ggctcgggcg atccagtggc ggtgctccat gggggccccg
      841 gggccgatca cggttacctg ctccccggct tcgatcttct cgccgaaggg cgggagctga
      901 tctactacga ccagcggggt gggggccggt cgcccgttcc gcgcgatacc agggtgggct
      961 ggcgggagca tgtcgcggat ctggatgcgc tgcgtgagct gtgggggctc gagcgcctgg
     1021 tcatcgtcgg gtattcctgg ggcggcctgc tcgcgcagtt gtacgccacc gagcatccgg
     1081 cccgggtcac gcggctcgcg ctggtctcgc ccgggccgtc atggcgcgct gcgcgcgatg
     1141 aattcgaacg ccgttttgcg cagcgaaacc tggccccgga gcttcaggcc gagcgcgagg
     1201 cggtccgggc aagcgatctg cgagtccgcg acccagaagc gtttcagcgg cgcatgttcg
     1261 agctttcggt tgttccgtac ttcttcgatc ccggaaaggc gcgggagctc actccattcc
     1321 gggtggtcgg ccgcacccag gaagaggtct ggaaaagcct gggcaactat gatctgcgat
     1381 cgaggcttcc ggcccttcga tccactccgt cgctcatcct ccacggtgaa gacgatccga
     1441 ttccgatcga ggcctcccgc gaaacggcga atctgctagg ggctgcgttt cattcgctcg
     1501 accgctgcgg tcactgcccc catgtcgagc ggcccaggga gttcaaggaa atcgtaagcg
     1561 ggttcttgtc aggatgaccc gaattcgtgt cgcgttcgaa accaactttc ctgctgacgc
     1621 gtaagcgagc catgcccttt cgtctcatgc gatttctctg cctgctgttg cccatcgccg
     1681 ccacgccggt tctggcccag caagactccg tattcgcgcc gctccacgcc gtggaccggg
     1741 ccaatttcga taccacgtgt gcgccgtgcc gcgatttctt cggctatgtc aatgggggct
     1801 ggctcgctcg caccaccatt ccgccgcagt actcgatcac cggcgtcgac cgcgacatcc
     1861 aggatcgtac ggaagcgctg ctgcgtcgga tcctggataa ggcggcgcgg gatgcgaaga
     1921 acgcgaccga cgccgacacg cgacggatcg gcctgtttta cggaagctgc atggactcgg
     1981 ttcgagtgga ccgggaaggg gcgaagccgc tgcgaagccg gctcgacagg attgccgcaa
     2041 tccggtcccg ggcccagctg gtgggcgtca tggccgagct cgagcgcgag ggggtcaacg
     2101 gcccaattcc ctaccttgcg tacccgaatt tcaagaacag cagggtgatg gccttccatc
     2161 tctaccaggg cggcctggga ctcccggatc gcgacttcta cctgcgaaac gattcgcaat
     2221 tcaccgcgat acggcgggaa tacacagccc atgtgggccg tatgctcgca ctgctcgact
     2281 cgcctaagga ctccgctgat gctgacgcca ggcgcgtcgt ggcgctcgag accgcgatcg
     2341 cgcgaacgtc catcccggcg caggatgcgc gggcgttcag ccgtctctac caccctaccg
     2401 cgcgagcaag cttgctgcga atggcgcccg atatcgactg gaccggattc ttcagcgcga
     2461 ccgcggtcgg cgcgccagat acgatcaacg tggcgatccc ggggttcatc cgaggcgtcg
     2521 acagcctgat ccgcactgcg tcaattgccg actggcaagc ctatctgcgc tggcagctgg
     2581 ccactgcaac ggcaccggcc ttgagcaccc ccttccagca ggaggcgctg gtccttcgcc
     2641 gcctcactcg aggcgagacc cagctcaagc cgcgatggca gcgctgccag caggccaccg
     2701 accaggcgat cggccaggcg ctcggcaggg agtacgtgaa agcagcgttt ccacccgagg
     2761 caaaggcgcg gatgcaggca atgatcgcaa acctgcgagc cgcgatgcgg gatcggctga
     2821 acacgctcac ctggatgagc gactccacca agggcgaggc gttcaagaag ctcgatgcgg
     2881 tctcgcccaa gatcggatac ccggacaagt ggcgggacta ctc
//
LOCUS       DASRJQ010000139         3445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1918981, whole genome shotgun sequence.
ACCESSION   DASRJQ010000139 DASRJQ010000000
VERSION     DASRJQ010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1918981"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1846)
                     /locus_tag="VFB89_03195"
     CDS             complement(83..1846)
                     /locus_tag="VFB89_03195"
                     /EC_number="2.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC and FAD-binding oxidoreductase
                     domain-containing protein"
                     /protein_id="HZM26334.1"
                     /translation="MARLLSVNVGLPRDIEWKGRTVHTAIWKNPVSGRCRVGRLNLEG
                     DGQGDLAGHGGEQRAVFVYQIESYRYWQERLKRTDFVPGQFGENLTVEGLPDDVVCIG
                     ERYQIGGALFEVTQPRVTCYRVGIRMNEPSMPALLTSSGRPGFYFRVLQEGDVGAGDE
                     IVKVGEAQERMTVAAINALLYSPNHPQDRLERALRIEALSPGWRGSFEALLRSQTIGA
                     GSGNAGLAPAAAAYPVPPGFRSLAVTAIDLESADVLSLTLQSRDSQPLPMPLPGQYVV
                     LRLQRTAGGPPLFRSYSLSGPLSTERYRISVKIESNGAAGMYLRERVRVGDTLDVSSP
                     RGSFILQSGERPVVLLSAGIGATPVLAMLHALSADRSTRPVLWLHGARDGQHDPFAGE
                     VRGLMGALTHARSYVCYSRPDSRDRLGDNFDAAGHLSGPVFDQVGVPRDADVYLCGPT
                     PFMAEMKAALAALGVPPERIQVELFNGSEPITPGVVGAARRAPHLPKDDANTGPLLSF
                     ARSGIAAHWKASAYQSILELAEACDVPVRWSCRTGVCHSCESGLVWGAVVYGPEPLDK
                     PADGNLLVCCSQPIRDVVIDL"
     gene            complement(1846..>3445)
                     /locus_tag="VFB89_03200"
     CDS             complement(1846..>3445)
                     /locus_tag="VFB89_03200"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM26335.1"
                     /translation="GEPPFTGPSAQMVIAKRFTDPVPRVRRLRPTVPAAVEQALLKAL
                     ARVPADRFASAGAFAEALTAPAARERPPASVAVLPFLNLSPDPENEYFADGMTEDVIA
                     QLSKIRALKVTSRASAMVFKKREQSLRQISDTLQVATVLDGSVRKAADRVRIVAQLID
                     VDSDQQVWTETYDRKLTDIFAIQTDVALQIAAALEAQLLPEEHTRIRKEPTSDIEAYK
                     LYLLARHCVVRWTHEGFELALKHLEQAVARDPNYALAYATMAYVYTDIGVGVAGKLPP
                     DQAFRRAKAAVARALEIDSGLAEAHAVLGHIKYAYDYDWEGAEEELKRAIELNPNSGD
                     AYDMYGLMLSALDRYDEAIEMQRRAHELDPLAHRMDMATTCLRAGRYDEALSAVIKIV
                     EVDPHVALAHATLGWAYLLKGMSEQGLAALERAVSLSPDSTMYLAQLGHAYARVGQTA
                     KAREILQRLEEMAKDHYVSPYHLAYVHTGLGEQDRAMDWLERAYEERAGAVFGIKGSF
                     LFTTLRSHPRFNALLRKMNLPIDG"
BASE COUNT          630 a         1114 c         1072 g          629 t
ORIGIN      
        1 gttactcaac tttgctgctt ggaccagacg tccctggcgg tcgacggtga cagtacttga
       61 gtcggaaatc gaatctcagg tttcacaaat cgatgacgac gtcgcggatt ggttgtgagc
      121 agcaaacgag aaggttgccg tcagcgggct tgtcaagtgg ctccggtccg tagacgaccg
      181 ccccccaaac caacccgctc tcacagctgt gacaaacacc ggtccggcac gaccaacgga
      241 ccgggacgtc gcacgcctcg gcgagctcca agatgctttg gtaggccgac gccttccagt
      301 gtgcagcgat gccactgcgc gcgaacgaca gcagggggcc ggtgttggcg tcgtccttgg
      361 gcaggtgcgg cgcccgcctc gcagcgccga cgacaccagg ggtgatgggc tcgctgccat
      421 tgaacagttc gacctgaatc cgttccggcg ggacgcccaa ggctgccagc gccgctttca
      481 tctctgccat gaagggcgtt ggcccgcaaa ggtagacatc ggcgtcgcgc gggacgccaa
      541 cctggtcgaa gaccggcccc gacaggtggc cggctgcgtc gaaattgtcg ccgagcctgt
      601 cgcgtgagtc cggcctgctg taacagacat aactccgggc gtgcgtgagc gcgcccatga
      661 ggccgcgtac ttccccggca aagggatcat gctgtccatc gcgagccccg tggagccaca
      721 agacgggccg ggtcgagcgg tccgctgaga gcgcgtgcag cattgccagt accggcgtcg
      781 ccccgatgcc cgcgctgagc agcaccacgg ggcgctctcc ggactgcaaa atgaagcttc
      841 cgcgcggcga gctgacgtcg agggtatcgc caactctgac acgctcgcgg aggtacattc
      901 cagcggcccc attcgactcg atcttcacgc tgattcgata gcgctcggtc gagaggggac
      961 ctgagagcga gtagctgcga aatagcggcg ggccgccggc ggttcgttga agacgcaaga
     1021 ccacgtactg gcctggaagg ggcattggca gcggctgact gtcccggctc tgcagcgtca
     1081 gcgagaggac atccgcggac tcgagatcga tcgctgtcac agcgagcgat cgaaacccgg
     1141 gtggaaccgg atacgcagct gcggccggcg cgagccccgc gttaccactc cccgcgccaa
     1201 tcgtttggct ccgcagtagc gcttcaaaag acccgcgcca tccgggtgac agcgcctcga
     1261 tccgcaatgc acgctcgagt cgatcctgcg gatgattggg cgaatagaga agagcgttga
     1321 tcgctgcgac agtcatccgc tcttgcgcct ctcccacctt cacgatttcg tcgccggcgc
     1381 ccacatcgcc ttcttgcagg acccggaagt aaaacccagg ccgtccgctg gatgtcagca
     1441 atgccggcat actgggctcg ttcatccgaa tgccgacgcg atagcaggtg acgcgaggtt
     1501 gagtgacctc gaacagcgcg ccgccgatct gataacgctc gccaatgcac acgacatcgt
     1561 cgggcaatcc ctcgacagtc aagttctcac cgaactgtcc ggggacgaag tcggtcctct
     1621 tcagccgctc ctgccaatag cgatacgatt cgatttggta gacaaagacg gcacgctgtt
     1681 caccgccgtg cccggccaga tcgccttggc cgtcgccctc caggttcaat cggcccaccc
     1741 gacagcggcc agacaccgga ttcttccaga tcgcggtgtg caccgtacgc cccttccact
     1801 cgatgtcgcg cggaagtccg acgttcactg ataggagtcg agccattagc cgtcgatcgg
     1861 caggttcatc ttgcggagca acgcattgaa gcggggatgc gatcgaagcg tagtgaacag
     1921 gaaggagcct ttgatcccga acaccgcgcc agcacgttcc tcgtaggccc gctccagcca
     1981 gtccatcgct cggtcctgct ctcccaggcc ggtgtgaaca taggccaggt gataggggga
     2041 gacatagtga tccttcgcca tctcctccaa ccgctggaga atctcgcgag ccttcgcggt
     2101 ctgaccgacg cgggcatagg cgtggccgag ctgggcgagg tacatggtgc tgtcaggtga
     2161 gagcgacact gcccgttcga gggcggccag tccctgctcc gacatcccct tgaggaggta
     2221 agcccaccca agtgtcgcat gcgcaagcgc cacatgcgga tccacctcga cgattttgat
     2281 cacggcgctc agcgcctcat cgtacctgcc ggctcgcagg cacgtggtcg ccatgtccat
     2341 gcggtgagcc agcgggtcga gctcgtgagc acgccgctgc atctcgatag cttcatcata
     2401 gcggtcgagc gcggagagca tgagcccata catgtcgtat gcgtcgccgc tgttcgggtt
     2461 cagctcaatc gcccgcttga gctcctcttc cgctccttcc cagtcgtagt cgtaggcgta
     2521 tttgatatgc cccagcacgg catgagcttc agcaagccca ctgtcgatct cgagcgctcg
     2581 cgcgaccgcg gctttcgctc gccgaaacgc ttgatccggc gggagcttac cggccactcc
     2641 taccccaatg tcggtgtata cgtacgccat ggtggcgtac gccagcgcgt agttcggatc
     2701 ccgcgccacc gcctgttcga ggtgcttcaa agccagttcg aagccctcat gggtccaccg
     2761 gacgacacaa tgcctcgcta acaggtacag cttgtaggct tcgatgtcgg aggttggttc
     2821 tttccggatt cgagtgtgtt cctcgggcaa gagctgcgcc tcgagcgcgg cggcgatctg
     2881 cagggccacg tcggtctgaa tggcgaagat gtcagtcagc ttgcggtcgt aagtctcagt
     2941 ccatacctgc tgatcgctgt cgacatcgat cagttgcgcc acgatccgca cccgatcggc
     3001 ggccttccgc acgctgccgt ccagcacggt cgccacctgc agcgtgtcac tgatctggcg
     3061 gagactctgc tcccgcttct tgaataccat agccgaggcg cgcgaagtga ccttgagcgc
     3121 ccgaattttc gagagctgcg cgatcacgtc ttccgtcata ccgtcggcga agtactcgtt
     3181 ttccggatcc ggactcagat tgagaaaagg gaggacggcg acggatgccg gtgggcgctc
     3241 gcgggctgcg ggcgccgtga gcgcctctgc gaatgccccc gccgaggcaa atcggtcggc
     3301 cggtacccgg gcaagtgctt tgaggagggc ttgctccaca gcggcgggca ccgtgggccg
     3361 caggcgtcgc acccgaggga caggatctgt gaaccgcttg gctatgacca tctgcgcgct
     3421 ggggcccgtg aacggcggct cgccg
//
LOCUS       DASRJQ010000140         2908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1311241, whole genome shotgun sequence.
ACCESSION   DASRJQ010000140 DASRJQ010000000
VERSION     DASRJQ010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311241"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VFB89_03205"
     CDS             complement(<1..326)
                     /locus_tag="VFB89_03205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZM26336.1"
                     /translation="MRDEIVRVVLVDDHTIVREGLRLLLRSAPDIAVVGEAVSGLTAI
                     DVAERLRPDIVVLDLDMPGSDGMATLRGLEQALPEVRVLVLTVHAERERLVPLLEAGA
                     RGYLTK"
     gene            complement(439..1683)
                     /locus_tag="VFB89_03210"
     CDS             complement(439..1683)
                     /locus_tag="VFB89_03210"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZM26337.1"
                     /translation="MAPQTRRYTTVFMGPDLGTATAVAQWRVLAEVGQALSRCARPEM
                     GLPDVAGLVVPQAANWCVLYLPGDNEVAATLDVAHVEMGRAEEIRGRLRGRLTRLEGD
                     RRLLAELGVSGGQVTPVRFGSRTRGLLALASEAGDLEGAGDLEFGRALADRIGLELGC
                     TLAYRQTQRAIAAGELAVGIVSHDLGNPLATIQICANALLDPRPQPVAGIRQIAEIIQ
                     RSAAWMQQIRRDLLDRVHLDTGGLPLARESTAVSDVMGTVEKMFLPVAGEQGLEFAIE
                     CAADLPRIDADPGRLQQALSNLLGNAMKFTPAGGRVVLSARAVADQERGPAIRFAVSD
                     TGPGIPEQDLPHVFDWFWHSQAGERTGTGLGLAIARGVIEAHSAHLMVESAPGGGSTF
                     WFTLPACGTRAEGCHGGIEARG"
     gene            complement(1790..2293)
                     /locus_tag="VFB89_03215"
     CDS             complement(1790..2293)
                     /locus_tag="VFB89_03215"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZM26338.1"
                     /translation="MATLGRERFLREIKTTAALTHPHILPLFDSGEADGFLFYVMPYV
                     EGESLRNRLDREQQLPLTDALEITTEIADALGFAHARHIVHRDIKPENILLEAGHAVL
                     TDFGIAKAVTDAGADALTDTGLAIGTAQYMSPEQAAGRRQLEGRSDMYSLASRSMVAG
                     CHSRSVR"
BASE COUNT          545 a          945 c          908 g          510 t
ORIGIN      
        1 tccttggtca aatacccgcg agcgccggcc tccagcaacg ggaccagacg ttcccgctca
       61 gcgtgcaccg tcagaacaag cacccggact tcgggaaggg cctgctccaa gccgcgtagg
      121 gtggccatac catcgctccc aggcatgtcc agatcgagaa ccacgatgtc ggggcggagc
      181 cgctccgcca catcgatggc ggtgagacca ctgacggcct ctcccaccac cgcaatgtcg
      241 ggcgccgatc ggaggaggag acgcaggccc tcgcgcacga ttgtgtgatc gtccaccagg
      301 acgacacgca cgatctcatc tctcatgggt accccccgtc gagcgtaggg tcgggacgcg
      361 agttcggcgc tcagcgatac caactcgcca gggaaaacgc aggtcccccg cacccccgac
      421 atcgtcatgg ctggacgatt acccccgtgc ctctataccg ccgtggcatc cctcggccct
      481 ggtcccgcag gccggtaggg taaaccaaaa cgtgctgccc cccccgggcg cactctctac
      541 catgaggtgt gcgctgtggg cttcgatcac ccctctcgcg atcgcgagtc ccaatccggt
      601 accggttcgc tcccccgctt gtgagtgcca gaaccagtcg aaaacatgtg gcaggtcctg
      661 ttcgggaatg cctgggccgg tgtcgctcac cgcaaagcgg atggcgggac ccctctcttg
      721 atcggcgacc gctcgagccg acagcaccac ccgcccgccg gcaggagtga acttcatggc
      781 attgcccagg aggttggaca acgcctgttg caatcggccc ggatcggcat ctatgcgggg
      841 caggtcggca gcgcactcga tggcgaattc cagtccctgt tccccggcga ccggcaggaa
      901 catcttttcc actgttccca tcacgtcaga cacggccgtc gactcccggg cgagcggcag
      961 tccgccggta tccaggtgca ctcgatccag gaggtcacga cggatctgct gcatccaggc
     1021 ggcggagcgc tgaatgatct cggcgatctg gcgaatacct gcgacaggct gtggccgggg
     1081 atccaacaaa gcgttcgcgc agatttgaat cgtagcgagc ggattaccca gatcgtgaga
     1141 aacgattccc acggcgagct cacccgcggc gatcgctcgc tgggtttgac ggtaggcaag
     1201 ggtacacccc agctcgaggc cgatgcggtc tgccaacgct cggccgaatt cgaggtcgcc
     1261 cgcaccctcg agatcgccag cttcggaagc cagcgccagc aggcctcgcg ttcggctccc
     1321 gaacctgacc ggagtgacct gtcctccgga gaccccgagc tccgcgagca gccggcgatc
     1381 gccttccagc cgggtcagcc ggccgcgcaa gcgtccgcgg atctcctcgg cccgacccat
     1441 ctccacgtgg gccacgtcca gcgtggcagc cacctcgttg tcaccgggca ggtagaggac
     1501 gcaccagttc gccgcctggg ggaccaccag accggcgacg tcgggcaaac ccatctcagg
     1561 tcgggcgcat ctggagagtg cctggccaac ttccgcaaga acccgccact gcgcgacggc
     1621 agtcgccgtt cccagatcag gccccatgaa gaccgtcgta taccgccggg tttggggggc
     1681 caaattaact ccgacgcgag ccccccaagc gtccatctcg gagatcggcg gtagaagggt
     1741 cgcgcgataa tgggttagaa tctgacaaat gccaggcctc gtcggtgatt cagcgcacgc
     1801 tgcggctgtg gcagccggct accatggatc gcgaagcaag gctgtacatg tcgcttcgcc
     1861 cttccaattg tcggcgtccc gccgcctgct ctggactcat gtactgcgcc gtgccgatcg
     1921 caaggccggt gtcggtcaga gcgtccgctc cagcgtccgt cacggccttg gcgatgccaa
     1981 aatccgtcag tactgcgtgg ccggcctcga ggagaatgtt ttccggcttg atgtctcgat
     2041 gcacaatgtg ccgggcatga gcaaagccca atgcatccgc gatctcggta gtaatctcga
     2101 gcgcgtcggt aagcgggagc tgctgctcgc ggtcgagccg attgcgcaag gattcaccct
     2161 cgacatatgg catcacatag aacaggaagc cgtccgcctc gcccgaatcg aatagtggaa
     2221 gaatgtgagg atgggtaaga gctgcggtcg tcttgatctc gcggaggaaa cgctctcgcc
     2281 ctaacgtggc cagctatctg cgggcggagc accttaaggg cgagacggcg tgcgtggcgt
     2341 ggctcctcgg ctaaatagac aatggacatg ccaccgcggc cgagctctcg tacgatgcaa
     2401 tagcggtctc ggaggacgtc tgcgagctgg gctgggatgt cgcccaaggt gatgctcaat
     2461 gttgcgaaga ttctcgaatc tgctagtgcc gccacgcgtt cgccagcgac gcaacggatt
     2521 tttccgctca aactggtctg gtcgtccttc aaccatccct cacagttttg ccgggttaag
     2581 agtagaggcc tgtggagcgg ttcaccccga agccttggcc ggaggtggtt ccgctcaacc
     2641 gcgacctgga agtgaggtca gagtcagaca tcggtcccgg ctcattgtta gccacgtcga
     2701 cttcgacgtg tcgtcccaag cgcgcattta agcgcgacta gacgctcatg cttgaggtcg
     2761 tgcgtgaggt agactgtggc cagcaggaac gcgtgcgagc tcgagggcgg accgacaacg
     2821 ctctagcgaa gcagccctgt gttaggctgt cgcgcgacct gtggccaccg tcgagatgaa
     2881 gaagccgtcg gagattcggg ggggtggg
//
LOCUS       DASRJQ010000141         3782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_469477, whole genome shotgun sequence.
ACCESSION   DASRJQ010000141 DASRJQ010000000
VERSION     DASRJQ010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469477"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..463)
                     /gene="panD"
                     /locus_tag="VFB89_03220"
     CDS             complement(14..463)
                     /gene="panD"
                     /locus_tag="VFB89_03220"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HZM26339.1"
                     /translation="MNRNERIYGVVGDSLNSLVDAVWGDAMQRRLMKSKIHRATITAA
                     DLHYEGSLTVDQDLLDAADLVTYEEIQVVNVNNGSRFTTYVIPGERGSGTMQLNGAAA
                     RLGHPGDLVIVISYGLFSEAEAKGHVPRVVFVDERNRPVRTPLRAER"
     gene            complement(494..2251)
                     /locus_tag="VFB89_03225"
     CDS             complement(494..2251)
                     /locus_tag="VFB89_03225"
                     /inference="COORDINATES: protein motif:HMM:NF005114.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase"
                     /protein_id="HZM26340.1"
                     /translation="MSNPTIAEQLIETLRQAGVKRIYGVIGDSLNPIVDAVRRTEGIE
                     WILVRNEEAGAFAAAAEAHLTGQLAVCAGSCGPGNTHLIQGLYDAQRSGVPVLALASH
                     IPSTQIGTGFFQETHPERLFVECSHYCELVSQPSQMPRLARVAIQHAIGRGGVAVLVL
                     PGDVLHRESEHLTGEYMPLAEKGTVLPPTRQVRELADLLNAANTVTLFCGAGVQGAHV
                     EVMALAAKLQSPVGHSLRGKEWIQYNNRFDVGMSGLLGYGACYDATHQADLLVLLGTD
                     FPYDAFLPQAHTVQVDHDATRLGRRTQLELAVHGDVGATLRAVLPLVEQKSDPSFLER
                     MLREHVKTLEHVVNAYTHDIEHHLPIHPEYATRILDELAADDAVFTVDTGMCNVWAAR
                     YITPTGQRRVIGSFLHGSMANALPHAIGAQLAYPGRQVISMSGDGGLGMLFGELLTVA
                     FHDIPVKIVTFNNSALGMIKLEMMVDGFPSYQTDFAPVDYAAIAKAAGIFSVRAEQPG
                     DVRAALTEAFKHRGPALVDLVTDPNALSMPPHITGEQVKGFALAASKTVLSGGVGQML
                     ELARTNLRNFPVIRTLTPA"
     gene            complement(2311..2682)
                     /gene="panD"
                     /locus_tag="VFB89_03230"
     CDS             complement(2311..2682)
                     /gene="panD"
                     /locus_tag="VFB89_03230"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HZM26341.1"
                     /translation="MQRQLMKSKIHRATITAADLHYEGSLTVDQDLLDAADLVTYEEI
                     QVVNVNNGSRFTTYVIPGERGSRVMQLNGAAARLGHPGDLVILISYGLFTEAEAKRHV
                     PRVVFVDERNRLVRTPLRAER"
     gene            complement(2667..>3782)
                     /locus_tag="VFB89_03235"
     CDS             complement(2667..>3782)
                     /locus_tag="VFB89_03235"
                     /inference="COORDINATES: protein motif:HMM:NF015882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HZM26342.1"
                     /translation="NDKLQLYTACARVPPQYLLPVHIDCGTSNQQLMDAFVDEFVDAV
                     QSVFPGCCIDFKDWAGVDAVRLLERYRHRVCCYNGDIQGTAGVAVAGLFSALRITGGR
                     LSDQRILFLGAASAAIGMADLIATAITLEGPTLRQARQRISLFDSNGLLEPSRTDLYG
                     FQKPYAHPHAPTRDFLSAIESLQPTAIVGVSSNGKAFTREIIEAMARLNRRPIIFALS
                     SPTDHAECTAEEAYRWSEGRALYVPRVQFPEIVPAVGLAICATKAKRVPNEIFVEAAR
                     AVAEQVTQAELEAGLLYPPQSQILETEVKAAQRAAEIVFAHGLAGVGRPDDLRTFIQS
                     RLYRPETPLAPIGSKDRAKRPERARRQVAWEIPCSAS"
BASE COUNT          630 a         1253 c         1224 g          675 t
ORIGIN      
        1 cgcggtcgcg ccctcagcgc tcggcccgca gtggggtccg caccggacgg ttccgttcgt
       61 cgacgaacac gacccgcggc acatgccctt tggcttccgc ttcgctaaac agaccatacg
      121 aaatcacaat gacgagatcg cccggatgac cgagtctcgc cgccgccccg ttgagctgca
      181 tggtgccgga cccgcgctcc cccgggatca cgtaggtagt gaagcgtgag ccgttgttga
      241 cgttcaccac ctggatctct tcgtaggtga ccagatcagc tgcatccaac aaatcctggt
      301 cgaccgtcag cgagccctcg taatgcaggt cggccgcagt gatggtggcg cggtgaatct
      361 tcgatttcat gaggcggcgc tgcatggcat ctccccagac cgcatccacg agtgagttca
      421 gactgtcacc gaccacgcca taaatgcgct catttcgatt catcctcagg ctcgaggccg
      481 gacgcggggc ccgtcaggcc ggcgtcaggg tgcgaatcac cgggaagttg cggagattgg
      541 tccgcgcgag ctcgagcatc tgcccaacac ccccggatag aaccgttttc gaggccgcga
      601 gcgcgaagcc tttcacctgt tcgcccgtga tgtgcggcgg catcgagagg gcgttcgggt
      661 cggtcaccag gtccacaagt gccgggcccc ggtgcttgaa cgcttctgtc agggctgccc
      721 ggacatcccc cggctgctcc gcccggaccg aaaaaatgcc cgccgccttg gcgatcgcgg
      781 cgtagtcgac cggcgcaaag tcggtctgat acgacggaaa gccgtccacc atcatctcca
      841 gcttgatcat ccccaaggcg gagttgttga aggtgacgat cttcacgggt atgtcgtgga
      901 acgcgaccgt gagcagctcg ccgaacagca tgccgagccc gccgtcgcct gacatagaga
      961 tcacctggcg gccggggtag gcgagctggg ccccgatcgc gtgcggcaag gcattagcca
     1021 tcgagccgtg caggaaggac ccgatcaccc gccgttgccc ggtcggcgtg atgtagcgcg
     1081 cagcccacac gttgcacatc cctgtgtcca ccgtgaacac ggcgtcgtcg gccgccagct
     1141 cgtccagaat gcgcgtggca tattcggggt ggatggggag gtgatgctcg atatcgtggg
     1201 tgtatgcatt gaccacgtgc tcgagcgtct tcacgtgctc gcgcagcatc cgctccagga
     1261 acgacgggtc ggatttctgc tcgaccagcg ggagcacggc ccggagcgtg gccccgacat
     1321 cgccgtgaac cgccagctcg agctgcgtcc ggcggccgag tcgcgtggcg tcgtggtcta
     1381 cctggacggt gtgggcctgg gggaggaagg catcgtaggg gaagtcggtc ccgagcagta
     1441 ccagcagatc cgcctgatgg gtcgcgtcgt agcaagcgcc gtagcccagg agcccgctca
     1501 tgccgacatc aaagcgattg ttgtactgga tccattcctt gccgcggagc gagtgcccga
     1561 ccgggctttg gagcttcgcc gcgagcgcca tcacctcgac gtgagccccc tggactcccg
     1621 cgccgcagaa cagcgtgacc gtgttcgctg cgttcagcag gtccgccagc tcccggacct
     1681 gccgcgtcgg cggaagcacg gtccccttct ccgcgagtgg catgtactcg cccgttaggt
     1741 gctcggactc acggtggagg acgtccccgg gcaggacgag cactgccacc ccaccccgtc
     1801 cgatcgcgtg ctggatcgcc actcgggcga gccgcggcat ctgcgagggc tggctcacca
     1861 gctcgcagta gtgactgcac tccacgaaca gccgttcggg atgggtttcc tggaagaacc
     1921 cggtcccgat ctgggtcgaa gggatgtgcg aggccagggc gagcacgggc acaccgctcc
     1981 gttgcgcatc gtagagcccc tggatcaggt gggtgttgcc cgggccgcag ctgcccgcgc
     2041 acaccgccaa ttggccggtc aagtgggcct cggcggcggc cgcgaaggct cccgcctcct
     2101 cgttgcggac caggatccac tcgatccctt cggtccgccg gaccgcatcc acgattgggt
     2161 tcaggctgtc accgatcacg ccgtagatgc gcttcacgcc ggcctggcgg agggtttcga
     2221 ttaactgctc ggcaatggtc gggttgctca tttcgcttct atcctccggc tcgaggccgg
     2281 gcgtaggtga ttgctgtcgc gctcgcgccc tcagcgctcg gcccgcagtg gggtccgcac
     2341 cagacggttc cgttcgtcga cgaacacgac tcgcggcaca tgccgcttgg cttccgcttc
     2401 ggtaaacaga ccgtacgaaa tcagaatgac gagatcgccc ggatgaccga gcctggccgc
     2461 cgccccgttg agctgcatga cgcgtgaccc gcgctcgccc gggatcacgt aggtcgtgaa
     2521 ccgtgagccg ttgttgacgt tcaccacctg gatctcttcg taggtgacca gatcagctgc
     2581 atccaggaga tcctggtcga ccgtcagcga gccctcgtaa tgcaggtcgg ccgccgtgat
     2641 ggtggcgcgg tgaatcttcg atttcatcag ctggcgctgc atgggatctc ccacgccact
     2701 tggcgccggg cgcgctccgg ccgcttcgcc ctgtccttgg aaccgatagg tgccagcggg
     2761 gtttcggggc gatacagccg tgattgaatg aacgtgcgca ggtcatccgg gcgccccact
     2821 cccgcgaggc cgtgcgcgaa aacgatttcg gctgcccgct gagccgcctt cacctcggtc
     2881 tccagaatct gggactgcgg aggatacagc agccccgcct cgagttcggc ctgggtcacc
     2941 tgctccgcga ccgcgcgcgc cgcctcgacg aatatctcgt tcggcacccg cttcgccttg
     3001 gtggcgcaga tcgcgaggcc aacggccggg acgatctccg ggaactgaac cctgggcacg
     3061 tagagcgccc ggccctcgga ccagcggtac gcctcctccg ctgtgcattc ggcatgatcc
     3121 gtcgggctcg agagcgcaaa gatgatgggc cgccggttca ggcgcgccat tgcctcgatg
     3181 atctcccgcg tgaacgcctt gccgttggag ctcactccca cgatcgccgt cggctggagc
     3241 gattcgatcg cgctgagaaa gtcccgggtc ggtgcgtgcg gatgcgcgta gggcttctgg
     3301 aaaccataca ggtcggtccg gctcggctcg agcaggccgt tgctgtcgaa cagggagatg
     3361 cgctgccgtg cttgtctgag ggtggggcct tcgagcgtga ttgctgtcgc gatcagatct
     3421 gccatcccga tcgccgcgga cgctgcaccg aggaagagaa tgcgctggtc gctcaacctg
     3481 ccaccggtga tccggagcgc gctgaacagg ccagccaccg ccacgccggc ggtgccctga
     3541 atatcaccgt tgtagcagca cacgcggtgc cgataccgct ccaagagccg caccgcatcg
     3601 actccggccc agtccttgaa atcgatgcag cagcccggaa agacgctctg cacagcgtcc
     3661 acgaactcat cgacaaacgc atccatcagc tgctggttgc tggtgccgca gtcgatgtgc
     3721 accggcagca gatattgagg cgggacgcgc gcgcaggcgg tgtagagctg gagcttgtcg
     3781 tt
//
LOCUS       DASRJQ010000142         2568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1872938, whole genome shotgun sequence.
ACCESSION   DASRJQ010000142 DASRJQ010000000
VERSION     DASRJQ010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1872938"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2568
                     /locus_tag="VFB89_03240"
     CDS             <1..>2568
                     /locus_tag="VFB89_03240"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM26343.1"
                     /translation="GEVYRAHDEKLGRDVAVKVLPVEVAQDEVRLGRFRREAKMAAAL
                     DHPYICKIYELGDTDDGRLFIAMEYVEGETLQARLERGPLALNAGVRAGLEIAEALVK
                     ADERGVVHRDLKPANVMLSREGHVKVMDFGLAKPARVTEVATEAETLEELSGRGVIAG
                     TPGYMSPEQLRGATIDGRSDLFAFGVVLQEILTCTHPFRGDSGPDTIAAILREAPVGG
                     EELPSVVRQLVAELLSREPEKRPSLEQTRLQLEQWVAHPDLLAATAGPDRAFVGRETE
                     FAELRSLAERLRTGKGSVALVGGEPGVGKTRLAREILSHAGRQGLLALEGHCDEMEGA
                     QSYLPWIEGLELAIRLVPEPRLRDALGEAAAEVAKLVPELRYRFQEIIPAIDLPPGLQ
                     QRYLFNSIGGFLDRLSASAPIVWLFDDLHWADDSTLLLFQHLLPRLEDLPILFIGTYR
                     DVDLDVGKPFERILAQLVRQRVGHRITLRRLSEGSTAELIQGLAGRTPPSPLVHMIYR
                     ETEGNPFFVEEVFQHLREEQRLFDKDGNWIDGIAISELDVPEGVRLVIGRRLERLKEA
                     AVRVLRAAAVIGRVFDPRVLESIDSLDPEDVLDAIEEAEAARLITTAMGDQYSFTHEL
                     IRQTLMAQQSLPRRQRMHLQIAGALEKSLAARAEEKAIDIAHHLVLAGAAADSMRTVR
                     YLVRAGDNALAAAAPREAFHFFEAALSRCHRLADGDSRVQALEQAVKVFQKRGLLRFT
                     AGDFGGAIGDFDRMLADARRLGDRRLESTALAYRGWIEWWNHEFDKAELTAREALELA
                     GEHMDDVRFFASSTLGLMFYGTHRTDEAEPHLRAAEQLAPTVDDPLTRSWWSVVGWHR
                     "
BASE COUNT          449 a          787 c          894 g          438 t
ORIGIN      
        1 gggcgaggtc taccgggctc atgacgagaa gctgggacgc gacgtcgcgg tcaaggtgct
       61 gcccgtcgag gtggcgcagg atgaggtgcg gctggggcgc tttcgtcgcg aggccaagat
      121 ggcggcggcg ctggaccacc cctacatctg caagatctac gagctcggtg acaccgacga
      181 cgggcgtttg tttatcgcca tggagtatgt ggaaggggag acgctgcagg cacgactgga
      241 gcggggacct cttgcgctca acgccggcgt ccgcgcgggc ctggaaatcg cagaagcgct
      301 ggtcaaggcg gatgaacgcg gcgtcgttca ccgcgacctg aaaccggcga acgtgatgct
      361 gagccgcgaa gggcacgtca aggtcatgga cttcggtctt gcgaagccgg cccgtgtcac
      421 ggaggtcgct accgaggcgg agaccctgga agaactgagc gggcggggcg tgatcgccgg
      481 aacgcccggc tacatgtctc ctgagcagct tcgaggggca acgatcgatg gacgcagcga
      541 cctgttcgcg ttcggcgtcg tgctgcagga gattctgacc tgcacgcatc ccttccgagg
      601 ggacagtggc cccgatacga tcgctgccat cctaagggag gcgcccgtcg gcggcgagga
      661 attgccttcc gtcgtccggc agctcgtcgc cgagctcctg tccagagagc ccgagaagag
      721 gccttcgctc gagcagacgc gcctccaact tgagcagtgg gtggcacacc cggaccttct
      781 ggccgcgact gctggtccgg accgcgcctt cgtgggccgc gagactgagt ttgcagagct
      841 gcggagcctg gccgagcgac tgcggacggg caaagggagt gttgcgctcg tcggcggtga
      901 gccaggtgtc ggcaagacgc ggttggcgag ggagatcctc tcgcatgccg gcagacaggg
      961 cctcctcgcc ctcgaggggc actgcgatga gatggaggga gcgcagtcct acctgccgtg
     1021 gatcgagggc ctggagctgg cgattcgcct cgtaccggaa ccgcggctgc gggacgcgct
     1081 cggagaagcc gcagcggagg tcgccaaact ggtccctgag ctacgctatc ggttccagga
     1141 gataataccg gccatcgacc tgccgccggg gctacaacag aggtatctgt tcaatagcat
     1201 tggcggcttt ctcgaccggc taagcgcgag tgccccaatc gtctggctct tcgatgatct
     1261 gcattgggcg gacgattcga cgctccttct gttccagcat ctgctgccaa gactcgagga
     1321 tctgccgatt ctctttatcg gcacatatcg agatgtcgac ctagacgtcg gcaagccgtt
     1381 cgagcgcatc ctggcccagc tcgttcgtca gcgcgtcggc catcgcatca cgctgcgccg
     1441 gctctcggaa gggtccacgg ccgagctcat ccagggcctt gccggtcgca cgccgccgag
     1501 cccgctcgtt cacatgatct acagagagac cgaaggaaac ccgttcttcg tggaagaggt
     1561 gttccaacac ttgcgggaag agcagaggct cttcgacaag gacgggaact ggatcgatgg
     1621 gattgccatc tcggagctcg acgttcccga aggcgttcgt ctcgtgatcg ggcggcgtct
     1681 ggagaggctg aaggaagccg cggtcagggt tctccgggcg gcagcagtga tcggccgagt
     1741 cttcgatcct cgtgttctgg agtcgatcga cagcctcgat cccgaggacg tgctcgatgc
     1801 catcgaggag gcggaggctg cgcggctaat caccacggca atgggcgacc agtactcgtt
     1861 cacccacgag ctcatccggc agacgctgat ggcacagcag tcgctaccgc gccgccagcg
     1921 catgcacctg cagatagcgg gcgcgctgga aaagtctctg gccgcgaggg ccgaggaaaa
     1981 ggcaatcgac attgcgcacc acctggtgct tgccggcgcc gcggcggatt cgatgaggac
     2041 cgtgcggtat ctggtccgcg cgggcgacaa cgcgcttgca gcggccgctc ccagagaggc
     2101 gttccacttc ttcgaagcgg ccctctcccg gtgccaccgt ctggctgatg gggactcacg
     2161 cgtccaggcg ctcgagcagg ccgtgaaggt cttccagaag cgaggcctgc tgaggttcac
     2221 cgccggcgac ttcggcggcg cgatcggaga cttcgatcgg atgctcgcgg acgcccggcg
     2281 actcggtgac cggcggctgg agagcacggc tctggcctat cgtgggtgga tcgagtggtg
     2341 gaaccacgag ttcgacaagg cggagctcac cgcgcgggag gccttggagc tggccggtga
     2401 gcacatggac gacgttcgct tctttgccag ctccaccctc ggcctgatgt tctatggcac
     2461 ccacaggacg gacgaggccg agccccacct ccgggcggcc gagcagctcg ctccaacagt
     2521 ggatgatccc ctcacgagga gctggtggag cgtggtcggt tggcaccg
//
LOCUS       DASRJQ010000143         4244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3005135, whole genome shotgun sequence.
ACCESSION   DASRJQ010000143 DASRJQ010000000
VERSION     DASRJQ010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3005135"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VFB89_03245"
     CDS             complement(<1..664)
                     /locus_tag="VFB89_03245"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HZM26344.1"
                     /translation="MATTDATLNGTFQARLSAARRAHRLPLELRQRAEEFGHALLAYL
                     FPHFARVTRTDQSDIAAEESHLRALLAPMLEDGQGQANVFFAGLPPIYDALLADADAM
                     YQGDPAANGRDEVILAYPGFFAIALYRAAHALHDLEVPLFPRLLTEVAHRETGVDIHP
                     AAQIGSRFAIDHGTGIVIGETAVLGDRVRIYQGVTLGAAAVRKELSRIKRHPTIGNNV
                     VIY"
     gene            761..1156
                     /locus_tag="VFB89_03250"
     CDS             761..1156
                     /locus_tag="VFB89_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HZM26345.1"
                     /translation="MSAPSPVPPPVPATGTGLTPNLAGALAYLLGPITGIIFLILEKT
                     NTFVRFHAMQSTVLWVGWVIASIALSILSGVVPVIGWIFGLVLSIVMSFGGFILWLVL
                     MWKAYQGQEWELPIVGPFARKQVSASTSV"
     gene            complement(1134..3188)
                     /locus_tag="VFB89_03255"
     CDS             complement(1134..3188)
                     /locus_tag="VFB89_03255"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HZM26346.1"
                     /translation="MTVSARGRIRLWAGLVFATACSSGRTSAPSTTPVPAEGSAATEA
                     PAAPTSAGGIPAAYRLEAPEWWAHAIAQGTRTTTGAPGPNYWQQYASYKLEAELNPVL
                     KRLTGRGRVVYYNRSPDSLPVVYVQAYQNLFREEAKRNQQTPKLGGIDFDRVMAEGKI
                     LSSVGKPTEPGYTVTGTIMELRLPKALAPHDSLVMEFDWQFRVPSETAPREGQDGEVW
                     FIAYWYPQLAVYDDVNGWQIDQYLGRGEFYMGYADYDVKLTVPTGWLLDATGTLENAT
                     QVFTPQTLARLDSARTGTTIVHVVTEKDKADSVATTKGADGKLTWHWTARNVRDFVWG
                     TSPRYLWDAARAVTDSAKGKITSSSLVSAFYRPEAVRSYWDEAARYGRHSVEFFSKKL
                     WPYPYPHMTAVDGPEGCGGMEYPMLTCIGGTYDSLSLYEVVTHEIGHMWFPMMVGSDE
                     KRYGWQDEGFTQYDQSQAMADFFKGFDDEARNRDYYLRGAQAGYEEEMMKPADRYGND
                     QSYGIAAYWKPATVLVALREALGRETFEKAFREYGRRWLGKHPTPWDFWNTFDNVTGQ
                     DLSWFWKEWFFETWKLDLALDTVQIEGDSAAIVMENRGKAIMPVPLAITREGGGVERV
                     MVPVSVWFGGEKRYTLKVAAKPAITKVEIDPEHGYPDSNRGDEIWSRGRAASSVTPTS
                     KR"
     gene            complement(3477..4088)
                     /locus_tag="VFB89_03260"
     CDS             complement(3477..4088)
                     /locus_tag="VFB89_03260"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HZM26347.1"
                     /translation="MTATKLLDNLHKDRTRRDPTQAARAGNLNRLPALPERGSTIRYT
                     VLDSPVGRIMIAATEHGVCALFLGDTDAALEADLRTEFPDARILPDSSGLGEWADRIS
                     ACLEGRVPRADIRLDARGTPFQHRVWDHLRAIPAGQTRTYTEVAEALGVPRGARAVGR
                     ACATNPVSVIVPCHRVVRGDGNLAGYRWGIARKRALLEWERRR"
BASE COUNT          725 a         1377 c         1330 g          812 t
ORIGIN      
        1 cgtagataac cacgttgttt ccgatcgtcg ggtgccgctt gatgcgcgac aattccttcc
       61 tgacggctgc cgcccctagc gtcacgccct ggtagatcct cacccggtct cccagtaccg
      121 ccgtttcccc gatgacgatg ccggttccgt gatcgatggc gaaccggctt ccgatctgcg
      181 cggccgggtg aatgtcgacc cccgtttccc gatgggcgac ctcagtgaga agtcgaggaa
      241 agagcggaac ttccaggtcg tggagagcgt gcgccgcccg atacagcgcg atcgcaaaga
      301 aacccggata cgcgaggatc acctcgtccc ggccatttgc cgccggatca ccctggtaca
      361 tggcgtcggc gtccgccagc aaggcatcgt aaatcggtgg aagcccggcg aagaacacat
      421 tcgcctgccc ctgcccgtct tcgagcatcg gcgcgagcag agcccggagg tgggattcct
      481 ccgcggcgat gtcggattgg tcggtacgag tgacccgggc aaaatggggg aagagatagg
      541 cgagcaacgc atggccgaac tcttcggccc gctgacgcag ctcgagcgga agccggtgcg
      601 cccggcgggc agcagacagt cgagcctgga atgttccgtt cagggtcgcg tcggtcgtag
      661 ccatcttctc aaattagcga gaacggagca tcctgtattg acagccgccg ggacgagcca
      721 caccttcggc tcacttcccc tacgccgctt ggaggatcag atgtccgctc cgtcgccggt
      781 ccctccccca gtgcccgcta ccggaaccgg cctgacgccg aacctcgccg gggcgctcgc
      841 ttatctgctg ggtccgatca ccgggatcat cttcctcatc ctcgaaaaga ccaacacgtt
      901 cgtgcgattt cacgccatgc agtccaccgt gctgtgggtc ggatgggtca tcgccagcat
      961 cgcgttgtcg atcttgagcg gcgtagttcc ggtgatcggg tggatattcg gattggtcct
     1021 ctcgatcgtg atgagtttcg gcggcttcat cctgtggctg gtgctcatgt ggaaggcgta
     1081 ccagggccag gaatgggagc taccgatcgt cgggccgttc gcccgcaagc aggtcagcgc
     1141 ttcgacgtcg gtgtaacact cgaagcagcg cggccccggg accagatctc gtcgccgcga
     1201 ttgctgtcgg gatacccgtg ctccgggtcg atctcgacct tggtgatggc cggcttggcg
     1261 gccactttga gggtgtagcg cttctcgccc ccgaaccaga cgctgacggg caccatcacc
     1321 cgctctaccc cacccccctc gcgcgttatg gcgaggggga ctggcattat cgccttgccc
     1381 cggttttcca tcacgattgc ggctgaatcg ccctcgattt gtactgtgtc gagagcgaga
     1441 tcgagtttcc aggtctcgaa gaaccattcc ttccagaacc aggacagatc ctgtccggtc
     1501 acgttgtcga aggtgttcca gaagtcccag ggcgtggggt gcttgcccag ccagcgccgg
     1561 ccgtactccc ggaatgcttt ctcgaaagtt tcccgcccga gcgcctcccg gagcgcgacc
     1621 agcacggtgg ccggtttcca gtaggcggcg atgccgtagc tctgatcgtt gccgtagcga
     1681 tcggccggct tcatcatctc ctcttcgtac cctgcctgcg cgccgcgaag gtagtagtcc
     1741 cggttccgcg cctcatcgtc gaagcccttg aaaaagtctg ccatggcctg ggactggtcg
     1801 tactgggtga agccctcgtc ctgccagccg tagcgctttt catccgagcc gaccatcatc
     1861 gggaaccaca tgtgaccgat ctcgtgcgtc accacttcgt agagagatag gctgtcgtag
     1921 gttccgccga tgcaggtgag catggggtat tccatgccgc cgcatccttc cggtccgtca
     1981 accgcggtca tgtgcggata cggatacggc cacagcttct tcgaaaagaa ctcgactgag
     2041 tgccgaccgt agcgggccgc ctcatcccag tatgaccgca ctgcttcggg gcgatagaac
     2101 gccgatacca gcgacgagct cgtgatcttg cccttcgcgc tgtcggtgac ggctcgggct
     2161 gcatcccaca ggtacctcgg cgacgtcccc cagacgaaat cccggacgtt tcgggcggtc
     2221 cagtgccagg tcagcttgcc gtcggcgcct ttcgtcgtgg cgacgctgtc tgccttgtcc
     2281 ttctctgtga ctacgtggac gatggtcgtg ccggtgcgcg ccgagtcgag ccgagcaagg
     2341 gtttggggcg tgaagacctg agtcgcattc tcgagtgtac ccgttgcatc gagcagccag
     2401 cccgtgggca cagtgagctt gacgtcgtag tcggcgtagc ccatgtagaa ctcgccccga
     2461 cccagatact gatcgatctg ccaaccgttc acatcgtcgt agaccgccag ctggggatac
     2521 cagtaggcga tgaaccagac ttcgccatcc tgaccttccc gtggggccgt ttccgacggg
     2581 actcggaact gccaatcgaa ctccatcacc agcgaatcgt gcggcgccag ggcctttggc
     2641 agccgaagct ccatgatggt gccggttacc gtgtagcccg gctcggtggg cttgcctacc
     2701 gagctcagga tcttgccctc ggccatcacc cgatcgaaat cgatgccgcc gagcttcggg
     2761 gtctgctgat ttcgcttagc ctcttcgcga aagagattct gataggcctg gacatacacc
     2821 acgggaagcg aatcgggcga ccggttgtag tagaccacac gcccgcggcc ggtgagccgc
     2881 ttgagcacag gattgagctc ggcctcgagc ttgtagctgg catattgttg ccagtagttg
     2941 ggtcccggcg caccggtcgt ggtgcgggtg ccctgcgcga ttgcgtgagc ccaccactcc
     3001 ggtgcctcaa ggcgatacgc ggcggggatc ccgcccgcgg acgtgggcgc tgccggcgcc
     3061 tcagtggcgg cggagccttc ggcgggaaca ggggtggtag acggcgctga cgtgcggccg
     3121 gagctgcacg ccgtggcgaa caccaggccg gcccaaagcc ggatccgacc tcgagcagag
     3181 acggtcatag ggaatcgaaa gaggggtcca accaacctac ggtcgcgccg gtggctgcgg
     3241 tttcgactgg ggctgccggg tcgcacggac gcgcgggccc gcgcccgcca gacggcggga
     3301 aggcggcaaa catagggagg gaactctaaa cggtaccgcc cgaacaatga accgcggcct
     3361 cagtctcggt gccgcggcaa gttcatcgtg caacgctgcg ccccacccgg gtcttctctc
     3421 cgccgggact ggccctgaga gccgacccga tcaaacgctg gggctcgagt ccgctgtcac
     3481 cggcgccgtt cccactcgag cagtgcgcgc tttcgcgcta ttccccaacg gtatccagcc
     3541 agattgccgt cgccccgtac cacccggtga cacggcacaa tcaccgatac cggattcgtg
     3601 gcgcaggcgc ggcctacagc ccgggctcct cgaggaactc ccagcgcctc agcgacttcg
     3661 gtgtaggttc gagtctgacc cgccggaata gcgcgcaaat ggtcccacac gcggtgctgg
     3721 aatggagtgc cgcgcgcatc gagcctgatg tctgcccggg gcacgcggcc ctcgaggcag
     3781 gcgctgatcc tatccgccca ttcgccgagc ccgctcgagt ccggcaggat ccgggcatcg
     3841 ggaaactcgg tccggaggtc ggcttcgagt gcggcatcgg tatcgcccag aaacaacgca
     3901 cacacgccgt gttcagttgc cgcaatcatg atccgaccaa ccggcgagtc caatacggtg
     3961 tatcggatcg tggatccacg ttccggaagc gccggcaggc ggtttaggtt tccggcgcgg
     4021 gcggcttgag tcggatcgcg ccgggtacgg tccttatgca aattgtcgag aagtttcgtc
     4081 gcagtcatag tttcctcccg cgacaatatt gggacgcagg aggcatggct ggctatccgg
     4141 atcttgcgat tcaattcgcg agctcgactg tcgccctccc gggcgtgggc ccgactcccg
     4201 ctgagccgtc tcccttttca tgattggtcg attcacaccg cccg
//
LOCUS       DASRJQ010000144         3149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3086200, whole genome shotgun sequence.
ACCESSION   DASRJQ010000144 DASRJQ010000000
VERSION     DASRJQ010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3086200"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VFB89_03265"
     CDS             <1..434
                     /locus_tag="VFB89_03265"
                     /inference="COORDINATES: protein motif:HMM:NF015019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HZM26348.1"
                     /translation="FLGVFFGTLAFAFLEPYFFVGYLVSIALFGLYQAIFMANAGGAW
                     DNAKKIVEVQLKQKGTPLHAATVVGDTVGDPFKDTSSVALNPIIKFTTLFGLLAVELA
                     VSLTAQEGSGLSHALAAVFFVISMAFVYRSFYGMRIEKETA"
     gene            552..1601
                     /locus_tag="VFB89_03270"
     CDS             552..1601
                     /locus_tag="VFB89_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HZM26349.1"
                     /translation="MATTLSPPTVPAALTRTQLLELYYYMRLTRTLEERLVALYRQTK
                     VVGGLFRSLGQEAVAVGSAYALDRSKKDILSPLIRNLGSMLVQGARPLEILLQYMAKG
                     DSLSRGRELNIHIGDLERGFIGQISHLGDMVPVMAGVTLSFKLRKQPRVGLVYVGDGA
                     TSTGAFHEGINFAAVQHCPLVVIVENNGYAYSTPVGKQTAAERLADKAAGYGIPGLTA
                     DGNDVLAVYDVTKEAVDRARAGGGVTLIELITYRRKGHAEHDDQSYVPAGEIDRWATE
                     NDPLDRFVERLAESYAVDRSEVDALDVRVRDEIDQATALAEQSPAPVSAEALIGVYAD
                     PPVMPVQWYRERRHG"
     gene            1594..2571
                     /locus_tag="VFB89_03275"
     CDS             1594..2571
                     /locus_tag="VFB89_03275"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HZM26350.1"
                     /translation="MAEVTYLEAIRGGLVEEMERDPNVFCLGEDIGIYGGAFKVTEGL
                     LERFGADRVIDTPVSETGFVGAAAGAAHMGMRPVCEMQFIDFVSCAYSILTNYVATAR
                     YRSFLPCPMVVRGPSGGYVRGGPFHSQNPEAAFLHTPGLKLVCPATPADAKGLLKAAI
                     RDDDPVLFFEHKYLYRRIRGAVPDGDHVVPLGKARLAREGKDLSIITYAASVWKSLEA
                     AEILEREDGLSVEVVDLRTLLPMDDDAIVASVKKTNRALIVHEDTVTGGVGGEIIARI
                     NQLAFEWLDAPVRRVAAHDVPLPYAPALEDFVLPQTDDIVAAARWLAAY"
     gene            2610..>3149
                     /locus_tag="VFB89_03280"
     CDS             2610..>3149
                     /locus_tag="VFB89_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HZM26351.1"
                     /translation="MARIDVVMPQMGESIAEGTLSRWLKKAGDPVKRDEPIFEISTDK
                     VVAEIPAPGAGVLAEILVQEGETVPVQTVVGRIETEAGAAASSPAPVAAPAPAAPAQT
                     TASAPAAAPAPSKPSAPPKPPAPAPPKSEAPPVAAQAQPAPAGNGAVETADERLRRKS
                     TPLVRKMAAEHNLDLAAIPG"
BASE COUNT          572 a          964 c         1004 g          609 t
ORIGIN      
        1 tcttcctcgg cgtgttcttc ggcacgctgg cgttcgcatt tctcgagccg tactttttcg
       61 tggggtacct agtgtcgatc gccttgttcg gcttgtatca ggccatcttc atggcgaatg
      121 ccggtggggc ctgggacaac gcgaaaaaga ttgtggaggt ccagctcaag cagaagggca
      181 cgcccctgca cgctgcaacc gtggtaggcg acaccgtggg cgatccgttc aaggacacct
      241 cgtcggtagc gctcaatccg attatcaagt tcacgacctt gttcggtctt ctcgcggtcg
      301 agctggccgt ctcactcacc gcccaggagg gctccggctt gagccatgcg ctggccgcag
      361 tgttcttcgt aatctcgatg gccttcgtgt accggtcgtt ctacgggatg cggattgaaa
      421 aagaaaccgc gtaaacggta aacagtgaac agtgaacggg gggctctgct tgtaatggag
      481 tcccccgttt accgtttacc gtttacgctt taacttcctg ccggtttccc aaagtactca
      541 acgcctggtc tatggccacc accctgtccc ctcccaccgt ccctgccgcc ctcacccgca
      601 cgcaattgct cgagctgtac tactacatgc ggctcacccg cacgctggaa gagcgtctgg
      661 tggcgctcta tcggcagacc aaggtcgtgg gcgggttgtt tcggtcgttg ggacaggagg
      721 ccgtcgccgt cggctcggcc tacgcgctcg atcgtagcaa gaaagacatt ctctcgcccc
      781 tgattcgcaa tctgggctcg atgctggtgc aaggcgcccg accgctcgag atcctgctcc
      841 agtacatggc caagggcgac agtctgagcc ggggaaggga gctcaatatc catatcggcg
      901 atctcgagcg tgggttcatc ggccagatct cgcatctcgg cgacatggtt ccggtcatgg
      961 cgggcgtcac gctctcgttc aagcttcgga agcagccgcg cgtaggcctg gtttacgtcg
     1021 gcgatggcgc tacctcgacc ggcgcgttcc acgagggaat caatttcgcc gcggtgcagc
     1081 actgccctct cgtcgtgatc gtggaaaaca atgggtacgc ctattcgacg ccggtcggaa
     1141 agcagacggc ggctgagcgc ctggccgata aggcagcagg ctacggcatc cccggtctca
     1201 ccgccgacgg aaatgatgta cttgcggtct atgacgtcac aaaggaggcg gtggaccgag
     1261 ctcgggcggg gggaggcgtg accttgatcg agctcattac gtaccggcgg aaggggcacg
     1321 ccgagcacga tgaccagtcc tacgtgccgg cgggtgagat cgaccgatgg gcgaccgaaa
     1381 acgatccgct cgatcgcttt gtggagcgac tcgctgagag ctatgcggtg gaccgctccg
     1441 aggtcgacgc actggacgtt cgagttcggg acgaaatcga tcaggcgact gcgctggcgg
     1501 agcagtctcc agcgccggtg tcggcagagg ccttgattgg agtctatgcc gaccccccgg
     1561 taatgccggt gcagtggtat cgggagcgcc ggcatggctg aagtcaccta tctcgaggct
     1621 atccgcgggg gactcgtaga ggagatggag cgcgatccca atgtcttctg cctgggtgag
     1681 gacatcggga tatatggagg ggctttcaag gttaccgagg gactgctcga acgcttcggc
     1741 gccgaccggg tgatcgatac gcctgtgagc gagaccggct tcgtgggagc cgctgccgga
     1801 gccgcgcata tgggcatgcg accagtgtgc gagatgcagt tcatcgattt cgtctcttgc
     1861 gcttacagca tcctcaccaa ctatgtggcc acggcgcggt atcgcagctt tctgccgtgc
     1921 cccatggtgg tacgcgggcc cagcggaggc tacgttcgag gtgggccgtt tcattcgcag
     1981 aatcccgaag cagcgttcct ccacacgccc ggactcaaac tggtatgtcc cgcgacgccg
     2041 gcagacgcca agggactgct caaagccgcg attcgggacg acgatccggt gctgttcttc
     2101 gagcacaagt atctctatcg ccgtatccgg ggagccgtgc cggatggcga tcacgtcgtg
     2161 ccgttgggga aagctcggct ggctcgagag gggaaagacc tgtccatcat tacctatgcg
     2221 gcgtcggtct ggaaatctct cgaggccgct gaaatcctgg aacgcgagga cgggctttcc
     2281 gtcgaggttg tcgatctgcg tacgttgctg ccgatggacg acgacgcaat cgtcgccagc
     2341 gtgaagaaaa ccaatcgggc gctcatcgta catgaagaca cggtcactgg tggggtgggc
     2401 ggcgagatca tcgcccggat caatcagttg gccttcgaat ggttggacgc gccggtccgg
     2461 cgagttgcgg cacacgatgt ccccctcccg tacgcaccgg ctttggaaga cttcgtgctg
     2521 ccgcaaaccg acgatatcgt cgccgcggcg cgctggctcg ccgcgtactg agcggcgctg
     2581 atagctgata gctgctgacc ggagctcaca tggctcgtat agatgtcgtc atgccccaga
     2641 tgggcgagtc catcgctgaa ggaacactct cgcgctggct caagaaggcc ggtgacccgg
     2701 tcaagcgtga tgaaccgatt ttcgagatct cgactgacaa ggtcgtcgct gaaattcccg
     2761 cgcccggtgc cggcgtactc gctgagatcc tggtgcagga aggggaaacc gtgcctgtcc
     2821 agacggtggt gggccggatc gaaaccgagg ccggtgcggc tgcctccagt ccggcaccgg
     2881 tggctgcccc agcgcctgca gcacctgcac agaccactgc gtcggcccca gcggctgcgc
     2941 cggctccgtc gaagccgtct gcgccaccga agcctcctgc tccggcgcct cccaaatccg
     3001 aagctccccc cgttgctgct caagcccagc cggcgccggc cggaaacgga gccgtagaga
     3061 ccgccgacga acggctccgg cgcaagtcca caccactcgt gcgaaagatg gccgccgagc
     3121 acaacctcga tctggcggca atccccggg
//
LOCUS       DASRJQ010000145         4150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_179358, whole genome shotgun sequence.
ACCESSION   DASRJQ010000145 DASRJQ010000000
VERSION     DASRJQ010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179358"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VFB89_03285"
     CDS             complement(<1..790)
                     /locus_tag="VFB89_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZM26352.1"
                     /translation="MTDQLDTLLSEQRRFPPPAEFVAQAQAKADLYERARQDRLRYWA
                     EEASKLDWMAPWSEVLEWSLPHAKWFVGGKLNVSVNCLDRHLEGPRRNKAAIIWEGEP
                     GDRRVLTYWDLAREVNRCANALLALGVKRGDRVAIYLPMIPEAAISMLACARIGAIHS
                     VVFGGFSAEALRDRINDARAVALITADGGFRRGQVVPLKRFADQALTDCPSIRHVIVV
                     RRRTGGEGDESWASMQEGRDHWWHRLLDAASSECEPAAMDAEDML"
     regulatory      1017..1108
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1167..1481
                     /locus_tag="VFB89_03290"
     CDS             1167..1481
                     /locus_tag="VFB89_03290"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZM26353.1"
                     /translation="MTATRDKTRDLVAPQRLGLDTRVEFRKEAIQALEALPEGAGCLS
                     IDLSKTRQVDSAGLGVLMLIQRRAAERRQVVVLRQPNDEIRFLLAITKLAGLFTIEPA
                     TS"
     gene            1597..1965
                     /locus_tag="VFB89_03295"
     CDS             1597..1965
                     /locus_tag="VFB89_03295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZM26354.1"
                     /translation="MSSERRRRRVLIVEDEPALRLSYQRHFNPRYDLVFASTGAEALA
                     KVEAEPPDVAVLDMRLPDTDGVDLLRRIRIRHPTLPVIITTAYMSIEPQLKVLDLPHS
                     GYILKPFRLDELGARIDAVL"
     gene            1952..2668
                     /gene="ccmA"
                     /locus_tag="VFB89_03300"
     CDS             1952..2668
                     /gene="ccmA"
                     /locus_tag="VFB89_03300"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HZM26355.1"
                     /translation="MPSSDGSLLAAVGLERSFGHIRVLRGIDLRVGPGDALVVVGPNG
                     AGKTTLLRVLAGLTRPSAGEVWVLGKRLDRSTPETRRPIGFVSHQSMLYDDLTIAENI
                     SLAARLYGLADPRAKAARALESAGLAERGSARPRQLSRGLLQRAAIARALVHEPRLLL
                     LDEPFTGLDAPAAERLRELLQERKQRGLGLLLVSHHLDEAWNLATHVAVLGGGRWLLD
                     EAKNGPLAEFIPRYRALAHG"
     gene            2661..3341
                     /locus_tag="VFB89_03305"
     CDS             2661..3341
                     /locus_tag="VFB89_03305"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HZM26356.1"
                     /translation="MADGLRLALVIAAKDVRAELRSKTALLSATVFAALVLVIFNFAR
                     DPTALSATDLAPSALWVTFAFAAMVAMNRSFTVEREHGALDGLLLAPVPRGALFTGKM
                     LANLAFVAAVEAVALPLFILFFNVDLSRALPGIIGTAALATVGFVAVGTIFGAMAVRT
                     RFAELMLPVLLLPFMVPPLIGAVQVTSRLLAARPLSEMMGWLRLLAVYDVVFVTLAAL
                     AFSFVVEE"
     gene            3338..4060
                     /gene="ccsA"
                     /locus_tag="VFB89_03310"
     CDS             3338..4060
                     /gene="ccsA"
                     /locus_tag="VFB89_03310"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HZM26357.1"
                     /translation="MTGLSGAAPVVTLTRARAVVRRGIIISSIALIGIGVAAVLALGF
                     TPIEARQGLAQKIFYLHVPSAWSALLAFSLVGIAGGLYVWLEDPRLDLFAAASAEVGV
                     AFAAVMLTTGPIWAKPIWGTWWTWDARLTLTLFLFFLFVGYLALRAAVHDRHERARFS
                     AVVGIMGLLLVPFIHLSVYLFRTLHPQPIVLKPSAPSLPWEMLRTLLVSTVMFTLLYI
                     GYVTIRYGIGLAIDAREQSDAG"
BASE COUNT          647 a         1337 c         1339 g          827 t
ORIGIN      
        1 acagcatatc ctcggcatcc atcgcggccg gctcgcactc gctcgaggcg gcgtccagca
       61 gccgatgcca ccagtgatcg cgtccctctt gcatcgatgc ccaggattcg tcgccttcgc
      121 cgccggtgcg ccgccggacg acgatgacgt gccggatgct cgggcagtcg gtcagcgcct
      181 ggtcggcgaa gcgcttgagc ggtaccacct ggccacgccg aaacccaccg tcggccgtga
      241 tcagcgcgac cgctcgggcg tcgttgattc tgtcccggag cgcttcggcc gagaagcccc
      301 cgaaaaccac ggaatggatc gcgccgatac gcgcgcaagc cagcatggaa atggctgctt
      361 ccggaatcat cggcaggtag atggcgacgc ggtcgcctcg ctttaccccg agcgcaagca
      421 atgcgttagc acagcgattc acctcccggg cgaggtccca gtaggtgagg actcgccgat
      481 cgcccggctc tccctcccag atgatcgccg ccttgttccg acgggggcct tcgaggtggc
      541 ggtcgagaca gttgaccgag acgttaagct tgccaccgac gaaccacttc gcatgcggca
      601 gggaccactc gagaacttcg ctccatggtg ccatccaatc gagcttggaa gcctcttctg
      661 cccaataacg caggcgatct tgcctggcgc gctcatagag gtcggctttg gcttgcgctt
      721 gcgccacgaa ctccgccggc ggagggaagc ggcgttgctc gctgagcagg gtgtcgagct
      781 ggtcagtcat ggcaggaatt cttagaggga gcctcaaggt agggccgacc ggccccatca
      841 gccagagccg ggttgcagtt ctccaaactc tgtcgcggac tgcggtggat cgatgccgac
      901 atggcttgca gtggtgtcct ccatagccct ctgtaccaat ggcttaaggt gcttggcact
      961 gctctcgccc gtatgccggt cgggtcgcat ggtgcggccc gcgtacaaaa aaatccgccc
     1021 gataagagca cacccggagc gatccggggc cgcaaaacta gaggtctcat ccgtgagcgc
     1081 aggttgggtg agatagctcg gtgccgaagg tggacacagg ccagccgcag gtttgcgcgc
     1141 cagccttgtc gggtgtggga ggctctatga cggccacacg cgacaagact cgcgacctcg
     1201 ttgccccgca gcgtctgggg ctcgacaccc gagtcgaatt ccgaaaagaa gccatccagg
     1261 cgctcgaggc tctgcccgag ggagccggtt gcctgagcat cgatctgtcc aaaactcggc
     1321 aggtcgactc cgctggactc ggcgtcctga tgctgattca gcgtcgcgca gcggagcgcc
     1381 ggcaggtggt ggtgttgcgc caaccgaatg acgaaatccg ctttctgctg gcaatcacga
     1441 agttggccgg actgtttact atagaaccgg cgacatcgtg acgaagccac ccgtcccgac
     1501 tatggacggt gcttgatggc cgggttactg ttgagggtct aaccccctga tgtgcctgtg
     1561 ccccactgat atggggcgat gttcgaaggc ctgccaatga gctctgaaag acggcgccgg
     1621 cgcgtcttga tcgtcgagga cgagcctgcg ctccgcttga gttaccagcg ccatttcaac
     1681 cctcgctacg acctggtctt cgccagcacc ggggcggagg ccttggcgaa ggtcgaagcc
     1741 gagccaccgg atgtcgcggt gctcgatatg cgactcccgg ataccgatgg agtcgatctt
     1801 ctgcgacgga ttcgaattcg acacccgact ctcccggtca tcattacaac ggcgtacatg
     1861 agcatcgaac cgcagctcaa ggtgctcgac cttccccact ccgggtacat tctcaagcct
     1921 ttccgtctcg acgagctcgg cgcccggatc gatgccgtcc tctgacggca gcctgctcgc
     1981 tgccgtcggt ctcgagcgat cctttggcca tatcagggtg ttgcgcggta tcgatctccg
     2041 ggtcgggccc ggcgacgcac tcgtcgtggt cggtccgaac ggcgccggca agaccaccct
     2101 gttgcgggtg ctggctgggc tgactcggcc aagcgccggg gaagtctggg tgctcgggaa
     2161 aagactcgac cgctcaaccc cggagacccg tcgcccgatc ggattcgttt cgcaccagtc
     2221 gatgctctat gatgacctca cgatcgccga gaacatttcg ttggcggccc gactctacgg
     2281 gttggcggat cctcgtgcca aggcggcgcg cgcactggag tctgccgggc tggccgaacg
     2341 tgggtcggcc cgccctcgcc agctgagccg gggtttgctg caacgcgcgg cgattgcgcg
     2401 cgcgctggtc cacgagccac gcctgttgct gctcgacgag ccgttcacag ggctcgatgc
     2461 gccggcggcc gagcggttgc gggagctgct tcaggagcga aagcaacgag gactggggct
     2521 tctgctggtc agtcaccatc tcgacgaagc ctggaacctg gcgacccacg tcgccgtcct
     2581 gggcgggggc cggtggctgc tcgacgaggc gaagaacggt ccgcttgccg agttcatccc
     2641 gcggtaccgc gcgctggcgc atggctgacg gcctgcgcct cgctctggtc atcgcagcca
     2701 aggatgtgcg ggcggagcta cgcagcaaga ccgcgctgct ctccgcaacg gtcttcgcgg
     2761 ctctcgtgct cgtcatcttc aacttcgccc gggaccccac tgcgctgagc gcaacggatc
     2821 tggcgcccag cgcgctctgg gtgaccttcg cgttcgccgc gatggtggca atgaaccggt
     2881 cctttacggt cgagcgggag catggagcgc tggatggtct cctgctggcc ccggtgccga
     2941 gaggtgcctt gttcaccggg aagatgcttg caaaccttgc gtttgtcgct gcggtggaag
     3001 cagtcgcgct cccgttgttc attctcttct tcaacgtcga tctctcccgg gcccttcccg
     3061 gcatcatcgg cacggcggcg ctcgccacag tggggttcgt cgctgtcggt accatcttcg
     3121 gagcgatggc agtgcggact cgctttgcgg agctgatgct gccggtgttg ctccttccct
     3181 tcatggtgcc gccgctgatc ggcgcagtgc aggtcacgtc gcggttgctg gccgcgcgtc
     3241 cgttgagtga gatgatggga tggcttaggt tactcgcggt ttacgacgtg gtattcgtga
     3301 ctctcgccgc tctcgccttc tccttcgtgg tggaagaatg accggcctgt cgggcgccgc
     3361 tcctgtcgtc acgctcactc gcgcccgcgc cgtggttcgc cgcggcatca tcatcagctc
     3421 tatcgccttg atcgggatcg gcgtcgccgc ggtccttgcg ctcggattca cgccgataga
     3481 ggcgcgacag ggactcgccc agaagatctt ctatctccac gttccatcgg cctggagtgc
     3541 gctgctggca ttttcgctgg ttgggatcgc gggcggtctc tacgtgtggc tggaggatcc
     3601 ccggctagac ctattcgccg cggcatcggc cgaggtcgga gtcgcttttg ccgctgtgat
     3661 gctgaccacg ggaccgatct gggccaagcc gatctggggc acgtggtgga cctgggacgc
     3721 ccgactcacg ctgacgctgt ttctgttctt tcttttcgtc ggctacctcg cgctccgggc
     3781 tgccgtacac gatcggcacg aacgagcgcg gttcagcgcg gtcgtgggga tcatgggcct
     3841 gctgctggtc cccttcatcc acctgagcgt ctacctcttc cggacgctcc atccccagcc
     3901 gatcgtactc aagccgagcg cgccgtcgct accctgggag atgctccgga ccctgctggt
     3961 ctcgaccgtg atgttcacac tgctttacat cgggtacgtg accatccgct atggcatcgg
     4021 cctcgccatc gatgctcggg agcagagcga tgccggctga gccgccggcg accggcggct
     4081 acatggtggc cgcgtatgta gtggcgggga cgattctgtt gggatatttc ctgtcgcttc
     4141 tgaggcgggc
//
LOCUS       DASRJQ010000146         7227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1867150, whole genome shotgun sequence.
ACCESSION   DASRJQ010000146 DASRJQ010000000
VERSION     DASRJQ010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7227
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1867150"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /locus_tag="VFB89_03315"
     CDS             complement(<1..1317)
                     /locus_tag="VFB89_03315"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZM26358.1"
                     /translation="MYTLLIAALGRLLPAWRREWPEAMLAELAAISESAPRKRFGTGC
                     VRALFAEVVTARLGEWIAHPRTVGMAFAVGLVIAVLDQASDTRSPMWISLVATSALFA
                     WCRPAAAWRWGLLLAIGMPCLAAVSDTRGPYTFDRADAMYGMPPAILTAVVVASLRRH
                     LRLILHGLAGISLVAGARSATAQSAGVRRELTVGDIATFADSAFADYLRGSPQPSLAF
                     VVVKDGALVFAGGYGSEDAAGSRRVDPESTVFWMASLSKLVTTAAVMREVAGGRIALG
                     APAAQYLEWGLPSRKTWRAITVEDLLTHTSGLDEPFMRGAVNDLRHLVPLGDYFSRIP
                     WRAGTRPGDVLRYSNHGMALAGLIVERTSGMPFAEYVEREIFAPAGMTHSTFRQPVPA
                     ELGRRIATAGTGGAVDYLLPTPAGAMVGTASDMGRFLAAQLDTADSP"
     gene            complement(1317..1631)
                     /locus_tag="VFB89_03320"
     CDS             complement(1317..1631)
                     /locus_tag="VFB89_03320"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZM26359.1"
                     /translation="MRRPLRFSPATQLVLEHFAAAPSRWLHGYTLMTALDLPSGTLYP
                     VLMRLADSGWLETRWEPPAPGGRPPRHLYRLAGGAVAEARALLRKWAMRALNDGAPQE
                     AT"
     gene            2043..4313
                     /locus_tag="VFB89_03325"
     CDS             2043..4313
                     /locus_tag="VFB89_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZM26360.1"
                     /translation="METRTETLDQQIVTRYTGQPSRLPADLRRQIESAWDGPVMLYAF
                     ADLDPALQLAETWVALGATRVAVARPAAPSGAWEIDLFPRRRIGAIRESPGLSATTLT
                     LAAAPDEPPLAVLRYTNRQRRAFENLRFVLEEGLAERTVAVTDADREYAEAVAHPVRD
                     AQALVGGRPKAVIWRLLAYLRPYRRELTLGMAAATLITLVSLIPPWLAGYVLDRLVTP
                     VRNGALPLERAATLAWIAVSAMAAVFLVRQGAAVIRLRLMAIMGEWVARDLRTELYEH
                     LQSLSLSFFARKKTGSIITRVSADTDRLWEFLAFGVVDVSLSVVMLAGLGVVLVGLDW
                     RLGLAMVIPVPVFCWLIWRHGESINRLFLRAWRKWSRVSDVLSDTIPGIRVVKAFHQE
                     EREVRRFGERNEDVTAEFNRIHQMWTGFWPLLMLGVHATTVLVWVLAVPRLLGAAGPP
                     LSTGTFVAFLLYATMFIAPIEVIGQIARTMNRATTSAHRVFEVLDTEAEVTDRADAIR
                     LAPVRGRVSFEHVTFAYDGVRQVLRDVSFEVTPGEMIGLVGPSGGGKTTVVNLIARFY
                     DVTGGCVQVDGIDIRRLDSGDYRRQIGMVMQDPYLFHGTVLENIRYGLPDASLDRVIA
                     AARAANAHDFINKLTHGYDTVVGERGLTLSGGERQRVSIARAILHDPRILILDEATSS
                     VDTETERHIQEALDRLVAGRTVFAIAHRLSTLRKASRLFVFEDGRLIEQGTQSALLGK
                     EQGTYRRLHEMQLALN"
     gene            4396..4905
                     /locus_tag="VFB89_03330"
     CDS             4396..4905
                     /locus_tag="VFB89_03330"
                     /inference="COORDINATES: protein motif:HMM:NF020483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HZM26361.1"
                     /translation="MITTARLNATRYAPPGTDPVALRLGSDGRLWAGEGEAARAVVVR
                     RCFPWSEPARYISLRDENHREIALVRDPIELAEPSRGALLDAMAEAGFLFEVVRVFEI
                     EEEVEIRQWQVETKQGPRVFQTRLDDWPRALPGSGFLIRDVAGDLYRLPAPVTMDKRS
                     RELLWAFVD"
     gene            4983..5369
                     /locus_tag="VFB89_03335"
     CDS             4983..5369
                     /locus_tag="VFB89_03335"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HZM26362.1"
                     /translation="MLIYYDGLCGLCDRFVRFVVARDRRGRYRFAPLQGTTARTRLPE
                     LPDPAALTTVVLEDHGRFRVRSDAALAVVAGLGGAWRAAALLRVIPRRLRDAVYDWIA
                     RHRFRWFGRREECRIPGPDQRERFLP"
     gene            5366..5992
                     /locus_tag="VFB89_03340"
     CDS             5366..5992
                     /locus_tag="VFB89_03340"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HZM26363.1"
                     /translation="MTIGALLAKPWVDRTIAIIAALPFIYVLYYNVQIVELTIPRVAL
                     LLQLCLLVVTMLIRRAPVRVTTNPVYWLVAFVASYYGFLTVAVSGPGHPLVPARYTGV
                     LSIASVIADGYARVTLGRNIGFVPAQRRLVTGGPYRFVRHPIYSALFLAEFCVVLENF
                     SPVNLVLSLIFLSLFVIKTLMEENFLRLDPAYARYMEQVRYRWIPRVL"
     gene            6099..7007
                     /locus_tag="VFB89_03345"
     CDS             6099..7007
                     /locus_tag="VFB89_03345"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZM26364.1"
                     /translation="MNHPMRVVMRRTGLSADVLRAWEKRYKAVDPGRSEGGQRLYSDD
                     DVDRLVLLQRVTALGRNISQVAALRADQLRRLLLEDESALELPPAPNEDRTADRVRAE
                     AMDLVDALDGPELEKALRRGALRLGVDALVERAIVPLLRELGERCSRGEVTPAHEQVA
                     GGVVQRVLQWIGETSSRIAGAPCITLATTEGERDELGIQIVAAVAATEAWRVNYLGGD
                     LAADAIVRATRQTESQVLALSFGSPGSARDARAPLGTIRASLSPGVAVIAGGAGAAET
                     EADLSALGITVLPSLGELRAFLRSYA"
BASE COUNT         1160 a         2321 c         2509 g         1237 t
ORIGIN      
        1 gggcgagtcc gcggtgtcaa gttgtgcggc gaggaaccgg cccatatcgc tggcggttcc
       61 aaccatcgcg ccggcgggtg tgggaaggag gtaatcgaca gcgcctccgg tcccggcggt
      121 ggcgatccgc cttccaagct cggcaggaac gggctgccgg aatgtggaat gagtcattcc
      181 cgccggcgcg aagatctcgc gctcgacgta ctccgcgaac ggcatcccac tggtcctctc
      241 gacaatcaga ccggcgagcg ccattccgtg attcgagtat cgaagcacat cgccggggcg
      301 ggttccggcg cgccagggga tccgggagaa atagtcgccc aacggcacga gatgccggag
      361 gtcgttcacc gcacctcgca tgaagggctc atcgagcccg gacgtgtggg tgagcaggtc
      421 ctcaaccgtg atggcgcgcc aggtcttccg ggatggtaat ccccactcga gatactgcgc
      481 ggccggcgcg ccgagggcga tgcggccacc agcgacttcg cgcatcaccg ctgcggtcgt
      541 gacgagtttg gagagcgatg ccatccagaa gaccgtcgat tccggatcca ctctgcggct
      601 ccctgccgca tcctcgctgc cgtacccgcc cgcgaatacg agcgctccgt cctttacgac
      661 gacaaaggcg agactgggct gaggagaacc gcggagatag tcggcgaacg cagagtccgc
      721 aaaggtcgcg atatcgccga cggtgagttc gcgccgcacc cccgcggact gcgctgttgc
      781 gctgcgcgcg ccggccacga ggctgatccc cgcgagtccg tgcaggatga gccggaggtg
      841 acgccgtaag ctcgccacga caaccgcggt cagaatcgcc ggcggcatcc cgtacatggc
      901 gtcggctcgg tcgaaggtat aggggccgcg agtgtcgctc accgccgcaa ggcacggcat
      961 gccgatcgcc aggagcagtc cccatcgcca cgcggccgcg ggtcggcacc aggcgaagag
     1021 ggcactcgtg gcgacaagac tgatccacat cgggctgcgg gtgtcgcttg cctggtcgag
     1081 cacggcgatg acaaggccga ctgcgaacgc catgccgacg gttcgggggt gcgcgatcca
     1141 ctctcccagg cgcgcggtga cgacctccgc aaagagcgct cgcacgcatc cagtcccgaa
     1201 acgcttccgc ggcgcgctct cggagatcgc cgcgagctcg gcgagcattg cctcgggcca
     1261 ctcgcgccgc cacgccggaa ggagtcgacc gagcgccgcg atgagcagcg tgtacatcag
     1321 gtggcctcct gcggtgcgcc gtcgttcaat gcccgcatcg cccacttcct gagaagcgct
     1381 cgcgcctctg caacggcgcc gccggcgaga cggtacaggt ggcgcggggg tcgtcctccc
     1441 ggggccggag gttcccatcg cgtctccagc catccggagt cggcaagacg catcagcacg
     1501 ggatagagcg ttcccgacgg aaggtcgagc gcggtcatca gtgtgtagcc gtgaagccac
     1561 cgtgacggcg cggcggcgaa atgctcgaga acgagctgcg tagcaggcga gaagcggagc
     1621 ggacggcgca tggtctaaat ctacatatat cgagttattg gtcaagggcg ctcgcgtggc
     1681 cccggggctc ggtcaggacg tttcaccctc cctgaaactc ttgcccaagc attgcccgtt
     1741 tcttatgttg tctgacccgg gtaccaggtc ccgggcggta gccggaccaa aacaaggact
     1801 gcgctcacga tgcgaagcga cctgaccaag acctgcacca agatcccgat ctggggctcc
     1861 gggcacgaag tgcgcccgtt cgccgccggt ctggatagtg gccaggttgg cgatcgcatc
     1921 gctgctaccc ggagtaaacg agcaccttag gtcaagatca gatcgcgtga tcctccgcct
     1981 tccgggtgct ccgacagacc tggaaggctt ttcgttttgc aacactgaac gaggatcgcg
     2041 caatggaaac ccggactgag acgctcgacc agcagatcgt aacccgctac accggccagc
     2101 cctcgcggct gccggcggac ctccgccggc agatcgaatc cgcatgggac ggcccggtga
     2161 tgctgtacgc attcgccgac ctcgatcctg cgctccagct cgccgagacg tgggtggccc
     2221 tgggcgcaac ccgggtcgcc gtggcgcgcc cggcggcccc atcaggagcg tgggagatcg
     2281 atctctttcc gcgccggcgc atcggcgcga tccgggagag cccggggctt tcggccacga
     2341 cgctcaccct cgcggcggcg ccggacgagc cgcctctggc ggtgctgcgc tacaccaacc
     2401 ggcagcggcg cgcgttcgag aaccttcgct tcgtgctcga ggagggactg gccgagcgca
     2461 cggtggcggt caccgacgcc gaccgggagt acgccgaagc ggtggcgcat ccggtgcggg
     2521 acgcgcaggc cctggtgggc ggccgtccga aggcggtgat ctggcggctg ctcgcctatc
     2581 tccgccccta tcgccgggag ctcaccctcg gcatggccgc ggccaccctg atcaccctgg
     2641 tgagcctgat accaccgtgg ctcgcgggtt atgtgctcga ccgcctggtg acgccggtgc
     2701 ggaatggagc gctgccgctc gagcgggcgg cgacgctcgc ctggatcgcg gtgtcggcga
     2761 tggcggcggt gttcctggtt cgccagggcg cggcggtgat tcggctccgc ctgatggcga
     2821 tcatgggcga gtgggtggcg cgggacctgc gaaccgagct ctatgagcac ctgcagagcc
     2881 tgagcctgtc gttctttgcg cgcaagaaga ccgggagcat catcacccgc gtctccgccg
     2941 ataccgaccg gctctgggaa tttctcgcct tcggggtcgt ggacgtctcg ctctcggtgg
     3001 tcatgctggc gggcctgggc gtggtcctgg tcggactcga ctggcggctg ggcctcgcga
     3061 tggtgattcc ggtgccggtg ttctgctggc tgatctggcg gcacggcgag tcgatcaacc
     3121 ggctgtttct ccgcgcctgg cgaaagtggt cgcgggtcag cgacgtgctg tcggacacca
     3181 ttccgggcat ccgggtggtc aaggccttcc accaggaaga gcgggaggtg cggcgcttcg
     3241 gagagcgcaa cgaggacgtg acggcggaat tcaaccgcat ccaccagatg tggaccggct
     3301 tctggccgtt gctgatgctg ggcgtgcacg cgaccaccgt gctggtctgg gtgctggcgg
     3361 taccgcgcct gctgggcgcc gccgggcccc cgctctccac cggcaccttc gtggcgtttc
     3421 tgctgtacgc gacgatgttc atcgcgccga tcgaggtgat cgggcagatc gcgcgcacca
     3481 tgaatcgcgc cacgacgtcg gcgcaccggg tgttcgaagt gctggacacc gaagcggaag
     3541 tcaccgaccg ggcggacgcg atccggctgg caccggtgcg gggacgggtg agcttcgagc
     3601 atgtaacgtt cgcgtacgac ggcgtccgcc aggtgctgcg cgatgtgagc ttcgaggtga
     3661 cacccggcga aatgatcggg ctggtgggac cctccggcgg cggcaagacc acggtggtga
     3721 atctcatcgc ccggttctac gacgtgaccg gtggctgcgt gcaggtggat ggaatcgaca
     3781 tccggcggct ggacagcggt gattaccggc ggcagatcgg gatggtgatg caggatccct
     3841 atctgtttca cggcaccgtg ctggagaaca tccggtacgg actgccggac gcctcgctcg
     3901 accgggtgat cgcggcggcg cgcgcggcca acgcgcacga cttcatcaac aagctcactc
     3961 acgggtacga caccgtggtg ggcgagcgag ggcttacgct ctcgggcggc gagcggcagc
     4021 gggtgtcgat cgcgcgggcg atcctgcacg acccgcggat cctgattctg gacgaggcga
     4081 ccagcagcgt cgataccgaa accgagcggc atatccagga agcgctcgac cggctggtgg
     4141 cgggacggac ggtgttcgcc atcgcccacc ggctctcgac cctccggaag gcgtcgcggt
     4201 tgttcgtctt cgaggacggc cgcctgatcg agcagggcac ccagagcgcg ttgctgggga
     4261 aggagcaggg gacctatcga cggctgcatg agatgcagct ggcgctcaac tgatgctaac
     4321 ggctaactgc taacggctaa cggttgctgg cagttggcag ttagcggata gcagttagca
     4381 cttacgggag gtaccatgat tacgaccgca cgactaaacg ccacccgtta cgcgccgccg
     4441 ggaaccgacc cggtggcgct tcggctgggc tccgacggac gattgtgggc cggcgaagga
     4501 gaggcggccc gcgcggtcgt ggtgcggcgc tgctttccct ggtcggagcc ggccaggtac
     4561 atctccctgc gggacgagaa ccaccgggag atcgcgctgg tcagggaccc gatcgagctc
     4621 gccgagccgt cgcgcggcgc actcctcgat gcgatggcgg aggccggttt cctcttcgaa
     4681 gtggtacggg tgttcgagat cgaggaggag gtggaaatcc ggcagtggca ggtggagacc
     4741 aaacagggac cgagggtatt tcagacccgg ctcgacgact ggcctcgcgc gctgccggga
     4801 agcggcttcc tcatccgcga tgtggccgga gatctctacc ggctgccggc accggtgacg
     4861 atggacaagc ggagcaggga gctgctgtgg gcgttcgtgg actaatggtg gctaacagct
     4921 aactgctaac ggctaacgtc ggttagcggt tagcggttag cggttagcgg tagtttacac
     4981 ctgtgctcat ctactacgac ggcctctgcg gcctctgcga ccggttcgtg cgcttcgtgg
     5041 tggcgcgcga ccggcggggc cgttaccgct tcgcgccgct ccagggaacg accgcgcgca
     5101 cccgactccc ggagcttccc gaccccgcgg cgctgaccac cgtcgtgctc gaggatcacg
     5161 gccgctttcg ggtgcgttcc gatgcggcgt tggcggtggt ggccggactg ggcggcgcct
     5221 ggcgcgcagc cgccctgctg cgggtcatcc cccggcggct ccgcgatgcg gtctacgact
     5281 ggattgcccg tcaccggttc cgctggttcg gccggcggga ggagtgccgg atcccggggc
     5341 cggaccagcg ggagcgattc ctgccgtgac catcggcgcg ctgctcgcga agccctgggt
     5401 cgatcgcacg atcgccatca tcgccgcgct cccgttcatc tacgtgctct actacaacgt
     5461 gcaaatcgtc gagctcacca ttccccgcgt ggcgcttctg ctgcagctct gcctcctcgt
     5521 cgtcaccatg ctgattcgcc gcgcgccggt ccgggtgacg accaatccgg tgtactggct
     5581 ggttgccttc gtcgccagct actatggatt cctgacggtg gccgtgagcg gccccggtca
     5641 cccgctggtg ccggcgcgct acaccggcgt gctctcgatc gcgagcgtga tcgccgacgg
     5701 ctacgcccgg gtgacactgg ggcgaaacat cggctttgtg ccggcgcagc gccgcctcgt
     5761 gaccggcgga ccctatcgat tcgtacgaca cccgatctat agcgcgctct tcctggcgga
     5821 attctgcgtc gtgctggaaa acttttcgcc ggtgaacctg gtcctcagcc tcattttcct
     5881 cagcctcttc gtgataaaga ccctcatgga agagaacttt ctgcggctgg atcctgccta
     5941 cgcgaggtat atggagcagg tccgctatcg ctggatcccc cgagtactct gagctccctt
     6001 gcgtcattgg gtcagcgccg ttacgcccac gataaatgac ccattcttta tctcaaaaac
     6061 cttgacaata tctcgaattt ggctgatttt gagcaataat gaaccatcca atgcgggtag
     6121 tcatgcgccg cacggggctg tcagccgacg tgttgcgggc ctgggaaaag cgctacaagg
     6181 ccgtggatcc cggtcgctcc gaggggggcc agcggctcta cagcgacgac gatgtcgatc
     6241 gcctcgtgct gctccagcgg gtgaccgcgc tggggagaaa catcagtcag gtggcagccc
     6301 tccgggccga tcagcttcga cggttgttgc tggaagacga gtcggcgctc gagctgccac
     6361 cggcgcccaa tgaggaccgc acggcggacc gggtgcgcgc cgaagcgatg gatctggtgg
     6421 atgccctcga tggaccggag ctggagaagg ccctgcgccg cggtgccctg cggctcggcg
     6481 tggatgcact ggtcgaacgg gccatcgtgc cgctcctgcg ggaattggga gagcggtgca
     6541 gtcggggcga ggttactccc gcgcacgagc aggtcgccgg cggtgtcgtc cagcgagtgc
     6601 tgcagtggat cggcgaaact tcgagccgga ttgccggcgc gccctgcatc accctggcga
     6661 cgaccgaagg tgagcgggac gagttgggaa ttcagatcgt ggccgcggtc gccgccacgg
     6721 aagcgtggcg ggtcaactac ctgggcggcg atctcgctgc cgatgcgatc gtccgggcca
     6781 ccaggcagac cgagtcgcaa gtgctggcgc tgagcttcgg gtctcccggc agcgcgcgag
     6841 atgcccgcgc gccgctcggt accatccgtg cgagtctttc cccgggtgtg gcggtgatcg
     6901 ccggcggcgc cggtgcagcg gagactgagg cggatctcag tgcactaggc atcaccgtgc
     6961 tgccaagcct cggagagctt cgggcgtttc tgcggtcgta tgcctaggcg gaacggcgaa
     7021 acggcgcaaa gacgcaaagc agttcacctt gcgtcgttgc gtcgttgcgt ttgttattcc
     7081 gagactcagt gatccttctt gcgctggtcc gcgacgtaag cccggagatc ggacgctgcg
     7141 agttgagcca tctcgcccgg gcgccgatcg acgtcgtcca cgccgctcgg cagcacgcgg
     7201 catatctcga ggcgctccgt gccgcgg
//
LOCUS       DASRJQ010000147         4084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3841249, whole genome shotgun sequence.
ACCESSION   DASRJQ010000147 DASRJQ010000000
VERSION     DASRJQ010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3841249"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="VFB89_03350"
     CDS             complement(<1..1086)
                     /locus_tag="VFB89_03350"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HZM26365.1"
                     /translation="MTSVSGYQALDPCVHCGFCLSACPTYLATGDESDSPRGRILLMR
                     ALERGELAPSDEALGHHLDACLGCRGCEPVCPSGVGYGRGLEAAREQLFQSRGLPGRA
                     RLMLAVFRKAALWRLAFGGGRLLRRTGLPGRLAGGTRLGFAMGMLAATNGGRRKHAVV
                     GTRQERARTDAREGTRTAAGANGSVALFRGCVMDTLFAHVHAATRRTLEANGYEVRDV
                     AGQGCCGALHEHAGDRDSAAALAARNVAALLDSADYIVVNSAGCGALLKDYGHLLGTD
                     AAREFGAKVRDVSELLAEQGPRPGVPLGLEVAYDAPCHLQHAQRVHAAPLALLRAVPE
                     LDVRLLPGSDRCCGSAGIFSLLQPEMAR"
     gene            complement(1103..2308)
                     /locus_tag="VFB89_03355"
     CDS             complement(1103..2308)
                     /locus_tag="VFB89_03355"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZM26366.1"
                     /translation="MIFSRLRALLGPAAVDRDPDNLPRVTADSTDALALVCRLAHDES
                     WTIRVEGLRTWLPPDAPADLAVSTRGLDEVVSVSPADLVATVQAGASMDAVRRRLAEE
                     SMWLAVDPPGRPDRTLGSVIATGTSGPLRLGFGPIRDHVLGCTVVTGDGRTVHAGGRV
                     VKNVAGYDLTKLHVGGFGGFGIVTEAHLRLRALPRADVTLLSRGGRDNQSAVARDLLD
                     AGINPAALELISPSLAGESEWVLAARFLGVEAGVQAEVHRLPAETGAIWTQLAPDRTA
                     DFWTGLARGSLNGSVTFRLGVLPGGIDDALDLLGRELDTGVVSAGAASGSIRWTGDAG
                     VDRLRGVRRSCAAREIPLTLERAPWSVRHALGHFGAYREGVGQLVGRLRNTFDPRQCL
                     KVALEGSEK"
     gene            complement(2305..3735)
                     /locus_tag="VFB89_03360"
     CDS             complement(2305..3735)
                     /locus_tag="VFB89_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HZM26367.1"
                     /translation="MKPSLAEGLRRIVGPRWVRDRKAELATYRMDGLPTHESEPGIVV
                     IPGSRSEVVEILRLLHLLQIPFVARGAGTGLSGGALAESDAVLLTLTRMNRILRVDRR
                     ARRAVIQPGVVNARLSEAVAPLGLQYVPDPSSQAACTVGGNVAENAGGPHCLKYGVTT
                     NHVVELEVVLPDASMVRIGSPQGEAWGPDLVGLFVGSEGMFGVATEITVRLETIPSAV
                     RTLLADFPSVRSASEAVSAVIASGIVPAALEMMDQACVQAVEASIYAAGYPTDAAAVL
                     LVELDGTAAEVDAESALVEAMLRDHAARSIRSASSPTDRARLWQGRKKAFGAMGRIAP
                     DLAVQDAVVPRSALPDILDQVGEIRRRYGLSISNVFHAGDGNLHPNISFDRRDTGLAA
                     RVREASRAIMEACVAAGGSITGEHGVGNDKMGYMPWIFDAETLGAMQAVRRVFDPQER
                     CNPGKVLPERACREWRAVPESRRAGP"
     gene            complement(3732..>4084)
                     /locus_tag="VFB89_03365"
     CDS             complement(3732..>4084)
                     /locus_tag="VFB89_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26368.1"
                     /translation="FLDQAGTSPTAGILSEVRALFARYYELYRMLHDADPEDPELPDE
                     AVSLSFTISAALECELPLKNRLLVTRATRERFELLLRLIPALSTELESALRVHRRAYQ
                     NGKGSRHSVLPTES"
BASE COUNT          711 a         1426 c         1328 g          619 t
ORIGIN      
        1 gcgggccatc tctggctgaa ggagggagaa aatgccggcg ctcccgcagc agcgatcgga
       61 gcccggcagg agcctgacgt cgagctccgg aaccgctctc agcagtgcga gcggagccgc
      121 atgcactcgc tgggcgtgct gtagatgaca gggagcgtcg taggcaacct cgaggccgag
      181 cggcacaccc ggtcgcggcc cctgctcggc caggagctcg gaaacgtcgc gcaccttggc
      241 accgaattcc ctcgcggcgt cagtgccaag caggtgtccg tagtccttga gcagcgcgcc
      301 acaccccgcg ctgtttacca cgatgtaatc cgccgaatcc agcaacgcgg ccacgtttcg
      361 cgcggcgagg gccgcggcgc tgtcccggtc gccggcatgc tcgtggagcg cgccacagca
      421 gccctgtccg gctacgtcgc ggacctcgta gccgtttgct tcgagggtgc gccgggtagc
      481 cgcgtgcacg tgagcgaaca gggtgtccat cacgcagccg cggaagagcg caaccgatcc
      541 gttcgcgccc gctgccgttc gcgtcccttc cctcgcgtct gttcgtgccc gttcttgcct
      601 ggtcccgacg accgcgtgct tccgcctccc gccattcgtc gccgccagca tccccatcgc
      661 gaatccgagg cgagtccccc cggcgagcct cccgggaagc cccgtgcgac gtagcagtcg
      721 gccgcccccg aacgcgagcc gccaaagcgc cgccttgcga aacacagcca gcatgagccg
      781 cgctcgaccg ggaagaccgc gggactggaa cagctgctcc cgagctgcct ccaggcctct
      841 accgtatccg acacccgagg gacagaccgg ctcgcagccg cggcaaccca ggcaggcatc
      901 cagatgatgt cccagcgcct catcactggg tgccagctct ccccgctcga gggcgcgcat
      961 gagcagaatt cgcccgcgcg gactgtcgga ctcgtcgccg gtcgcaaggt aagtgggaca
     1021 ggcggacagg cagaatccgc aatggacgca gggatccagt gcctgatagc ccgacaccga
     1081 cgtcacgatc tctgctctgg cgtcactttt cactgccctc cagggccacc ttgaggcatt
     1141 ggcgcggatc gaaggtgttg cgcagccggc cgaccagttg acccacacct tcccggtagg
     1201 cgccgaaatg tcccagcgcg tgccgcacac tccagggcgc ccgctcgagg gtcagcggaa
     1261 tctcacgagc ggcgcaggat cggcgaacgc cgcgaagccg atcgacgccg gcatcgccgg
     1321 tccaccgaat cgagccgctc gccgcgccgg cagagacaac gccggtgtcg agctcgcgtc
     1381 ccaggaggtc gagcgcgtcg tcgatcccgc ccgggagcac gccgagtcgg aacgtcacag
     1441 agccgttgag gctgcctcgc gcaaggccgg tccagaagtc cgcggtccgg tcgggggcca
     1501 gctgggtcca aatggcgccg gtttcagccg ggagtcgatg gacctcagcc tgcacacccg
     1561 cctcgacccc aaggaagcga gcggcaagca cccattcact ctcaccggcg agcgacggtg
     1621 agatcagctc gagtgccgcc ggattgatcc cggcatcgag caggtctcgc gcgaccgcac
     1681 tctggttgtc ccgcccgcct cgcgagagaa gggtgacatc tgcgcgggga agcgctcgca
     1741 ggcgaagatg cgcttccgtg acgattccaa agccaccgaa tccaccgacg tgtagcttgg
     1801 tgagatcata gccggcgacg ttcttcacta ctcggccgcc tgcatgtacc gtgcgtccat
     1861 ccccggttac aacggtgcag ccgagcacat ggtcccgaat aggcccaaag cccagacgca
     1921 gtggaccgct ggtgccggtg gcgatcactg agccgagcgt gcggtcgggg cgacccggtg
     1981 gatccacggc cagccacata ctttcctcgg cgagacgccg gcgtactgcg tccatcgagg
     2041 cgcccgcctg gacggtcgcg accaggtcgg ctgggctcac cgagacgact tcgtcgaggc
     2101 cgcgagtgct gaccgccaga tccgccggcg cgtccggcgg cagccaggtg cgaagccctt
     2161 caacgcggat ggtccagctc tcgtcatgcg cgagccgaca gacgagtgcc agcgcatcgg
     2221 tcgagtccgc tgtcaccctg gggaggttat cgggatctct atcgacggcg gcggggccca
     2281 acagggcccg gagtcgggag aaaatcacgg gcccgctcgt cgcgactcgg gtacagcacg
     2341 ccactcacgg cacgcgcgct cgggtagcac cttgcctgga ttgcagcgct cctgcggatc
     2401 gaacactctc cgcacggcct gcatcgcgcc gagagtctcg gcgtcgaaga tccatggcat
     2461 gtagcccatc ttgtcgttgc ccactccgtg ctcgcccgtg atgctgcctc cggccgctac
     2521 acacgcctcc atgatggcgc ggctcgcttc ccggaccctg gctgcgaggc cggtgtcacg
     2581 acgatcgaag ctgatattgg gatgcagatt gccgtcaccg gcatggaaga cgttgctgat
     2641 ggacaggccg tagcgtcggc gaatctcgcc tacctgatcc aggatgtcgg gcaacgccga
     2701 acggggcacg accgcgtcct gcactgccaa gtccggcgcg atccgcccca tggcgccgaa
     2761 tgccttcttg cgcccctgcc aaaggcgagc ccggtcggtc ggtgaggagg cgctccgaat
     2821 ggagcgagcc gcgtggtccc gcagcatcgc ttcgaccagg gcggattcgg cgtccacctc
     2881 tgccgcggtc ccatccaatt cgaccaggag caccgcggca gcgtccgtcg gataacccgc
     2941 tgcatagatc gaggcttcga ccgcctggac gcaggcctga tccatcatct cgagagcggc
     3001 cgggacgatc ccagaggcga ttacggcgga gacggcctcg ctggcggaac gcacgctggg
     3061 gaaatcggcc aggagcgtgc gaaccgcaga gggaatcgtc tccaggcgta ccgtgatctc
     3121 ggttgcaaca ccgaacatgc cttcactgcc gacgaacagc ccgaccagat cagggcccca
     3181 ggcctccccc tgaggcgatc caatgcggac catgctcgcg tccgggagga ctacctcgag
     3241 ctccacgacg tggttggtgg tgactccgta cttgagacag tgggggccgc cggcattctc
     3301 ggcgacattt ccgccgacag tgcaggcagc ctggctggaa ggatccggca catactgaag
     3361 cccgagcgga gcgaccgcct cggacaaccg ggcgtttacc actccgggct ggataacggc
     3421 tcgacgcgcg cggcgatcca cccggagaat gcgattcatc cgcgtgagcg tgagaagcac
     3481 cgcgtccgac tccgccagcg cgccacccga aagtccggtc cccgctccgc gagcaacgaa
     3541 ggggatctgg agcaggtgaa gcagtcgaag aatctcgacc acctcgctcc tgctccccgg
     3601 aatgaccacg atgcccggct cggattcgtg ggtgggcagc ccgtccatgc gatacgtcgc
     3661 cagctcagcc ttgcgatcgc gcacccaacg gggcccgacg atgcgccgaa gcccctctgc
     3721 caggctgggc ttcatgactc cgtcggcagc accgaatggc ggctgccctt gccgttctga
     3781 taggcccgcc ggtgcactcg gagcgcactc tccagctcgg tgctgagtgc cggaatcagt
     3841 cgcagcagca gctcgaaccg ctctctcgtt gcgcgcgtca caagcaggcg attcttgagc
     3901 ggcaactcgc actccagagc ggcggagatg gtgaagctga gggagaccgc ctcgtccggg
     3961 agttcggggt cttccggatc ggcgtcgtgc aacatccggt acagctcgta gtaacgggcg
     4021 aagagcgcgc ggacctcgct caggattccc gccgtgggtg aagtgccggc ctggtccaga
     4081 aact
//
LOCUS       DASRJQ010000148         4137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2432135, whole genome shotgun sequence.
ACCESSION   DASRJQ010000148 DASRJQ010000000
VERSION     DASRJQ010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2432135"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1727)
                     /gene="tatC"
                     /locus_tag="VFB89_03370"
     CDS             complement(<1..1727)
                     /gene="tatC"
                     /locus_tag="VFB89_03370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HZM26369.1"
                     /translation="MPFLDHLEELRFRILRSLAAIAVGFGIGLWAVQHFTLVALLKRP
                     IEQYLTGGKLVVLSPTEPVLIVFKLGFVVGLVLASPVVLWQLWAFVAPALYEREKRAI
                     VPSLFAGLALFLTGSVFAWLFLVPKALAVLFSFQSEAIAPYITYDAYFDFVLQVVLSL
                     GISFELPLVIIILAWLGVVTPAILHRFRRYAVVLACIAGALLSPGTDLISMLMMTFPL
                     ILLYEVGFLGAVIIKRRRRKAAGTTAAILLAMLALPSRAHGQLPQVPGQQQGGIGGLV
                     PGIRPGQGIGIGQVGPSQPLDTATARLLGIPTAPTRQFPQEDSVITELLKRPGYKTTR
                     YKADSAIYYVEDRRIRLEGQALTEQQGIKLEADSVRYQEASCILEAGGEPHLFDKSQV
                     LVGGSISYNTCTRRGVIKNALTSFSAGQANWFIRGNVAADSSGSRLYAAKSEITSCDL
                     PTPHYHFVARQVKWVSKKAFVARPIVLYVRDVPIIWLPFMYQDIRPGRHSGILIPQFG
                     FADLIPTSPSYHRQISNIGYYWATNDYMDVTFRLDWFSSQYVQLGASMQYNVLNRFMG
                     GSFEVSRQF"
     gene            complement(1773..2579)
                     /locus_tag="VFB89_03375"
     CDS             complement(1773..2579)
                     /locus_tag="VFB89_03375"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM26370.1"
                     /translation="MSSTCWRSLALAAVAGVAACASSSDVQRVQNQVASLRAETARQD
                     SIRGARLSEIIRLQQRIMDSLGLAVRSLRGDTRQDLYNIQQQLVQLQELTGQSQQRLT
                     ELRTQLEARGAEIGATGTTPSEGTGGPGGTADTGRTAGPAPTASADQMYEASIAQLRR
                     GSTSTARIGFRRLVQTYPTSERVPDAVYFIGQSFAAENPDSAAAYYRQVLDRYAKSPR
                     AAAALYNLGLLAERRKDTAAARDAYQRVIRQYPQSNEAALARDRLKTLGR"
     gene            complement(2576..3136)
                     /gene="pal"
                     /locus_tag="VFB89_03380"
     CDS             complement(2576..3136)
                     /gene="pal"
                     /locus_tag="VFB89_03380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HZM26371.1"
                     /translation="MRASSILSVLAAAALLAACGKKPAPEQPAPEPPAPAHEEAPPPP
                     PPANDDAERLKREAEARAKALRGELTDLIHFEFDKADIRSTDQGILDRKAGILAANST
                     VRLQIAGHCDERGSDEYNLALGNRRAAAAKRYLTGKGVDEGRLDVVSYGKERPLDPGH
                     SEDAWARNRRDEFEITAGGENLTAPK"
     gene            complement(3151..>4137)
                     /locus_tag="VFB89_03385"
     CDS             complement(3151..>4137)
                     /locus_tag="VFB89_03385"
                     /inference="COORDINATES: protein motif:HMM:NF022113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HZM26372.1"
                     /translation="SKVTARLHDIVRSTLANEQLVGLPPPTDANFRLAVHVLSDEIAR
                     WATGVPGIAATRFLFVQNGRIWLMDSDGFLVRALTPDGQTSLSPAWSPDGRRMAFTRL
                     VQGRGGVFLYTLGTGETSQLPGTETGLNITPMFAPDGNHLAYAHSDEKGTDIYVATLG
                     QRNAQRLTAGRFADNLSPTYSPDGRRIAFISTRAGPPQLYAMAADGTDQELLAPFDFG
                     VTGPSYAPEWSPDGASVVFHREVSGVPQIFVLTVAGQKLRQLTSQGRNEDATWAPDGR
                     HLCFVSDRSGARQLWVIDAETGRVRQLATPGWARLPAWSRLLAGAAVVAPKP"
BASE COUNT          752 a         1308 c         1386 g          691 t
ORIGIN      
        1 tggaactggc ggctcacctc aaacgatccg cccatgaatc ggttcagcac attgtactgc
       61 atgctcgcgc ccagctgaac gtactgactg gaaaaccagt ccagccggaa cgtgacatcc
      121 atgtaatcgt tggtggccca gtagtaaccg atgttgctga tctgtcgatg gtatgacgga
      181 gaagtcggga tgaggtcggc gaagccgaac tgtgggatga gaatccccga atggcggcct
      241 ggtcggatat cctggtacat gaacggcaac cagatgatcg gcacgtcccg cacgtagagg
      301 acgattggtc gcgcgacgaa agccttcttc gacacccact tcacctgccg cgccacgaaa
      361 tgatagtgcg gggtgggcag gtcgcagctg gttatctcgc tctttgccgc gtacaggcgt
      421 gacccgctcg aatccgctgc gacatttccg cggatgaacc agttggcctg gcccgccgag
      481 aagctggtga gcgcattctt gatgacgcct cggcgggtac aggtgttgta cgaaatggat
      541 cccccgacca ggacctgact cttgtcgaac aagtggggct cgcctcctgc ttccaggatg
      601 cagctcgcct cctgatatcg cacgctgtcc gcttccagct tgatcccctg ctgctccgtc
      661 agtgcctggc cctcgagccg aatgcgccgg tcctcgacgt agtagatcgc ggagtcggcc
      721 ttgtagcggg tcgtcttgta accggggcgc ttgagcaact cggtgatcac cgagtcttcc
      781 tggggaaact gcctggtggg cgccgtcggg atcccgagga gccgagccgt tgccgtatcg
      841 agtggttgac tggggccgac ctgcccaatg ccgatcccct ggcccggcct gattccgggc
      901 acgagcccac cgatgccccc ctgctgctgt cccggcacct gcggcagctg tccgtgcgcg
      961 cgcgagggaa gcgccaacat cgccaagaga atggcggcag tggtgccagc ggctttccgt
     1021 cgccgacgct tgatgatgac cgctcccaga aagccgacct cgtatagcag gatcagcggg
     1081 aacgtcatca tgagcatcga gatgaggtcg gtcccgggcg agagcagcgc gcccgcgatg
     1141 caggccagta caacggcgta gcgccgaaac cggtgcagaa tggccggcgt gaccacgcca
     1201 agccaggcca gaatgatgat gacgagagga agctcgaacg agatcccgag cgagagcacc
     1261 acctgcaata cgaaatcgaa atacgcgtcg tacgtgatgt acggcgcgat ggcctcgctc
     1321 tggaagctga atagaaccgc gagcgccttg ggaaccagaa agagccaggc aaagaccgag
     1381 ccggtgagaa acagcgcgag cccggcaaac agggacggca cgatcgcacg tttctcacgc
     1441 tcgtagagcg caggggcaac aaacgcccag agctgccaga gcaccaccgg ggacgccaga
     1501 accagcccga cgacaaagcc cagcttgaaa acgatgagaa cgggctcagt gggactcagg
     1561 acgaccagct tgccgccagt gagatactgc tcgatggggc gcttgaggag tgcaaccagc
     1621 gtgaaatgct ggactgccca cagcccgatg ccgaagccca ccgcgatcgc agccagcgaa
     1681 cggaggatgc ggaaccggag ctcctcgagg tggtcgagga acggcatctc cccgctacga
     1741 tttaccatga acccagggcg aagcgcccgg gcttaacgcc cgagcgtttt gagacggtcg
     1801 cgcgccagcg cggcttcgtt ggattgaggg tactgccgga tcacacgctg gtaggcgtcc
     1861 ctggccgcag cggtgtcctt ccggcgctcc gcgagcaagc caaggttgta cagcgccgcg
     1921 gccgcccgcg gggacttggc ataccgatcg agcacttgcc tgtagtaggc cgccgcggag
     1981 tccggattct cggcggcaaa gctctggccg atgaagtaga cggcgtcggg cacgcgctcg
     2041 ctcgtggggt aggtctgaac cagccggcgg aaccctattc gcgcagtcga ggtgctgccc
     2101 cggcggagct gtgcaatcga cgcctcgtac atctgatccg cactcgcagt cggagcgggg
     2161 ccagcggtcc tgccagtgtc ggcggtgcca cccggaccac cagttccctc ggagggtgta
     2221 gtgcccgtgg cgccgatttc cgcgccgcgg gcctcgagct gggtccggag ctcggtcagg
     2281 cgttgctggc tctgccccgt gagctcctgc aactgcacca gctgctgctg gatgttgtag
     2341 agatcctggc gggtatctcc tcgcagcgag cgcaccgcga gacccagcga atccatgatg
     2401 cgctgctgca gccggatgat ttcgctcagc ctcgcaccgc ggatcgagtc ctgccgggcc
     2461 gtctcggctc gcagcgatgc tacctggttc tgcacccgct ggacgtcgct cgagctcgcg
     2521 cacgcggcga cgccagcaac ggcagcgagc gcgagcgaac gccagcaggt ggagctcact
     2581 tcggagccgt gaggttctcg cctccggccg taatctcgaa ttcgtcacgc cgattccgcg
     2641 cccaggcatc ttcactgtgc ccgggatcga gcgggcgttc cttaccgtag gagaccacgt
     2701 cgagtcgacc ctcgtccacg cccttccccg tcagataccg cttcgccgcg gccgcccggc
     2761 gattgccgag cgccagattg tactcgtcac tgccgcgctc gtcgcagtgg ccggcgatct
     2821 gaagccgtac ggtagagttc gccgcgagaa tgccggcctt tcgatcgagg ataccctgat
     2881 cggttgagcg aatgtcggcc ttgtcgaact cgaaatggat caagtcggtc aactcgccac
     2941 gcagggcttt ggcgcgagct tcggcctcgc gcttcaaccg ctcggcgtcg tcgttggcag
     3001 gtggcggtgg cggcggtgct tcctcgtggg ccggagcggg aggctcgggc gccggctgct
     3061 cgggcgccgg cttcttgccg caagccgcca gcagcgccgc agcagcgagg acgctcagga
     3121 tcgaggatgc gcgcatcagg actcctggaa tcagggtttg ggggcaacta ccgcggcccc
     3181 ggccaatagg cgggaccacg ctggaagccg ggcccagccc ggcgtggcaa gctgccggac
     3241 gcggccggtc tcggcgtcga tcacccagag ctggcgggca ccgctccgat cggatacgaa
     3301 gcacaggtga cgcccgtccg gggcccaggt cgcgtcctcg ttgcgcccct gggaagtcag
     3361 ctgccgcaac ttttggccgg cgacggtcag aacgaagatc tgcgggactc cggaaacttc
     3421 ccggtggaag acgacggagg cgccgtcagg cgaccactcc ggtgcgtagg atggcccggt
     3481 gacaccgaag tcgaacggcg ccagaagctc ctgatcggtc ccgtccgcag ccatggcata
     3541 cagttgcggc gggcccgcgc gagtggagat gaaggcgatg cgacgtccgt cgggtgaata
     3601 ggtgggtgac aagttgtccg cgaagcgacc ggcggtcaag cgttgtgcgt tgcgctgtcc
     3661 gagcgttgcc acgtagatgt cggtgccctt ctcgtccgag tgcgcgtacg ccagatgatt
     3721 gccgtcgggt gcgaacatcg gcgtgatgtt gaggcccgtt tcggtgcccg ggagctggct
     3781 ggtttcgccg gttcccaacg tgtagaggaa gacgccgccg cgcccctgga ccagccgcgt
     3841 gaacgccatg cgccggccgt cgggcgacca ggcaggagag agcgaggttt gtccgtccgg
     3901 cgtgagcgcc cgaaccagga agccatcgct gtccatcagc cagatgcgtc cgttctgtac
     3961 gaagagaaac cgggtcgccg cgatccccgg cacacccgtg gcccaacgcg cgatctcgtc
     4021 ggaaaggacg tgcaccgcga gtctgaagtt ggcgtcggtc gggggtggca gaccgacaag
     4081 ttgctcattg gcgagggtgg aacgaacgat atcgtgaagc cgagcggtca ccttgct
//
LOCUS       DASRJQ010000149         3134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1587967, whole genome shotgun sequence.
ACCESSION   DASRJQ010000149 DASRJQ010000000
VERSION     DASRJQ010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1587967"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VFB89_03390"
     CDS             complement(<1..440)
                     /locus_tag="VFB89_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HZM26373.1"
                     /translation="MTVQRIEQDFAGRRLIIETGRLAKQAAGSAVVQFGDTMVLAAIT
                     VSPTVSALPFFPLTVEYREKTYAAGKIPGGFLKREGRPSDKEILASRLIDRGIRPLFP
                     EGFKNEVQVFVTVLSADQENDADILGAVAASVALGVSTVPWNGPL"
     gene            complement(524..784)
                     /gene="rpsO"
                     /locus_tag="VFB89_03395"
     CDS             complement(524..784)
                     /gene="rpsO"
                     /locus_tag="VFB89_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HZM26374.1"
                     /translation="MPFDKQTVVAKHRRHDADTGSTRVQVALLTERINSLTDHFRTHP
                     KDHHGRRGLLKMVGTRRRLLQYLKRSDLPTYRALIEELGLRH"
     gene            complement(949..2313)
                     /gene="rseP"
                     /locus_tag="VFB89_03400"
     CDS             complement(949..2313)
                     /gene="rseP"
                     /locus_tag="VFB89_03400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HZM26375.1"
                     /translation="MLTTLLALAVVLGVLIFVHEAGHFLAAKWAGIYVHRFSLGLGSP
                     IRRLTFRRGETEYSVSWLPLGGYVKMASREEDATSALLEGGTAAPVPADRVFEAKPVW
                     KRMIVIVAGVTMNLVFAWAVYTYVAARHGQSIDPTTTVGLVLSDSLTGPMAPLAKLKP
                     GDRIVRVDGQIVSSWDDVVHHILSGQNNAGVTIDLADGRQVKLDLHPAAIEDRAHAVS
                     AILPFRSAVVDLAVGGRPAAKAGIAPGDSIVAVNGQSVTQRYDVLAIMDTSPGKPVTM
                     AVMRDGRRLDLSMKALAEQGIALDGKTRQVGRIGIRFRDPERTEPYTLFGAMRAGTEA
                     TLNSATFIVRTVQGLLTRRVSASNVGGPILIAQQAGEFARLGLDPFLEFMALISVNLA
                     VLNLLPVPVLDGGQLVMLFAEAVIRRPVPVALRERLTMVGLVVVVLLMVLAFSNDIRR
                     LLGG"
     gene            complement(2316..>3134)
                     /locus_tag="VFB89_03405"
     CDS             complement(2316..>3134)
                     /locus_tag="VFB89_03405"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HZM26376.1"
                     /translation="ALKAGKRVALASKETLVMAGDLVSAAARAGGGEIVPIDSEHSAV
                     LQCVAGHPTAVRRLIITGSGGPFRDWPADRVASASVSEALQHPTWRMGRKITIDSATL
                     ANKALEIIEAHHLFSLPYDALEAVIHPQSIVHAFVEFCDGSVIAQLGFPSMELPILYA
                     LTYPGRLPDRGVRTFDPVAAGPLTFEPVRTEVFRAFRAGVAAGRAGGTAPAVFNAANE
                     TAVAAFLDGCITFGRIHETIERTLDAHRQEPAADVETVRAADRWARRRAHELLR"
BASE COUNT          596 a         1033 c          975 g          530 t
ORIGIN      
        1 agcgggccgt tccacggcac ggtcgacaca cccagcgcga cggaggctgc cactgcaccc
       61 aggatatcgg cgtcgttctc ctgatcggcg gagagcacgg tgacgaaaac ctgaacctca
      121 ttcttgaaac cctcgggaaa cagcggccga ataccgcggt cgatcagccg gctggctagg
      181 atctccttgt ccgacggccg tccttcgcgc ttcagaaatc cgccgggaat tttcccggct
      241 gcgtaggtct tttcgcgata ctcgacggtg agggggaaga acggcagagc ggagacggtg
      301 ggggagacgg tgatagcggc gaggaccatc gtatcgccaa actgtaccac cgcggaacca
      361 gcagcctgct tcgccagccg gccggtctcg atgatgaggc ggcgcccggc gaaatcctgt
      421 tcgatccgtt gtacggtcac gtgtcaatcc ggttcagagc acaccaatgc agcgagggcg
      481 ccccgctgtg ctcgttcggg gcgccctcgc ggacccacag gccttagtga cggagaccga
      541 gctcctcgat cagcgcgcga taggtcggga ggtcgctgcg cttgagatac tggaggagtc
      601 ggcgacgcgt accgaccatc ttgagcagcc cccgccggcc gtggtggtcc ttggggtggg
      661 tgcggaagtg gtcggtgagc gagttgatcc gctccgtaag gagcgcgacc tggaccctgg
      721 tcgagccggt atcggcgtcg tgccggcggt gcttggcgac cacggtctgc ttgtcgaaag
      781 gcatactgcg gaggtcctcg ggcgtttcag cgtatcggtt ctatgttagc tcggcaaaat
      841 aacctacaga tggtgaaaag ggaaagggga agcccatctt ccaacccgcg cgaactgcag
      901 cgcccaaggc tcaccgcccc cgctgacttc ggtttcccct ttcccgtttc accctcctaa
      961 cagcctccgg atatcgttcg aaaacgcgag aaccatcagc agcacgacca ccaccagccc
     1021 caccatcgtc aggcgctcgc gtagagcgac cggaaccggt cgtcgaatga ccgcttcggc
     1081 gaacagcatc accaactgac ccccatcgag gaccgggaca ggcagaagat tgagtaccgc
     1141 caggttgacc gagatcagcg ccatgaattc cagaaaggga tccagcccca ggcgcgcgaa
     1201 ctcgcccgct tgctgcgcga tcaggatggg cccgcccacg ttcgaggccg agactcggcg
     1261 cgtgagcagc ccctgcacgg tgcggacgat gaacgtcgcc gagttgagcg tcgcctcagt
     1321 gcccgcgcgc atcgcgccga acagagtgta aggctcggtc cgctcaggat cccggaaccg
     1381 aatgccgatc cgacccactt ggcgggtctt tccgtcgaga gcaatcccct gctcggccag
     1441 cgctttcatc gagagatcaa gccggcgacc gtcgcgcatc actgccatcg tcaccggttt
     1501 gccgggactg gtgtccatga tcgccagaac atcgtaacgc tgggtgaccg attgtccgtt
     1561 cacggcgacg atcgaatcgc ccggcgcgat gccagccttg gccgccggcc gaccgccgac
     1621 cgccaggtct accacggcac ttcggaacgg aagaatcgcg cttaccgcat gagcccgatc
     1681 ctcgatagcg gctggatgga ggtccagctt gacctggcgg ccatccgcga gatcaatcgt
     1741 gacccccgcg ttgttctgcc cggacaagat gtgatggacc acatcgtccc acgacgagac
     1801 aatctggcca tcgacgcgta cgatccggtc gcccggcttg agcttggcga gtggggccat
     1861 aggccccgtc aggctatcgc tgagcaccaa tccgaccgtg gtggttggat caatcgattg
     1921 accgtgccgg gcagcgacgt aagtgtagac tgcccaagcg aagaccaggt tcatcgtgac
     1981 cccggcgacg atcacgatca tccgcttcca taccggcttc gcctcgaaga cgcgatcggc
     2041 tggcaccggt gccgccgtgc ccccttccag caacgcgctg gtcgcgtctt cctcgcggct
     2101 ggccatcttc acgtacccgc ccaacggcag ccaggacacc gaatactcgg tctctccccg
     2161 gcggaaggtc agacgtctga tgggtgaccc gagacccaag gaaaagcggt gcacgtagat
     2221 cccggcccat tttgccgcaa ggaagtgccc ggcttcgtgc acgaagatca gcactcccag
     2281 aacgacagcc agcgcgagca gtgtggtcaa cacggtcacc tcaacagttc atgagcccgc
     2341 cggcgagccc agcgatcggc agctcggacg gtctcgacgt ctgccgccgg ctcttgccgg
     2401 tgggcgtcca gcgttcgctc aattgtctcg tgaatcctgc caaatgtgat acacccatca
     2461 agaaacgctg cgaccgcggt ttcgttggcc gcgttaaaga cagcaggtgc cgtgccgccg
     2521 gcgcgcccag ccgccacacc tgcccggaag gcccgaaata cctcagtgcg taccggttcg
     2581 aaggtgagtg gaccagccgc gacaggatcg aaggtgcgta ccccccgatc cggaagtcgt
     2641 cccgggtatg tcagagcgta cagtatcggc agctccatgg acggaaagcc gagctgggcg
     2701 atgacgctcc catcgcagaa ctcgacgaaa gcatggacaa tgctctgcgg atggatcact
     2761 gcctcgagcg catcgtaggg caagctgaac aggtgatgtg cctcgattat ctcgagcgcc
     2821 ttattcgcca gcgtggcgct gtcgatcgtg atcttcctgc ccatccgcca ggtgggatgc
     2881 tggagggcct ccgacacgct cgccgaagcc acccggtcgg ccggccagtc acggaacggc
     2941 ccgcccgacc ctgtgatgat caggcgacgg acggcagttg gatgccccgc gacacattgg
     3001 agcacggcgc tgtgctccga gtcgattggt acgatctcgc cgccgccggc tcgggcggcg
     3061 gccgagacga ggtcgccggc catgaccagc gtctccttgc tcgcgagcgc cacccgcttg
     3121 ccggctttta gcgc
//
LOCUS       DASRJQ010000150         2633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3277841, whole genome shotgun sequence.
ACCESSION   DASRJQ010000150 DASRJQ010000000
VERSION     DASRJQ010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3277841"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2110)
                     /locus_tag="VFB89_03410"
     CDS             complement(<1..2110)
                     /locus_tag="VFB89_03410"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HZM26377.1"
                     /translation="MGSWSGSSVERLLRGVLRRRKRLFLIASLIAMVVMLPVAFIASK
                     EPPRYRATAVVLLEARPDRGVPVFQEMSPNRPFPVQMAILASRSLAETVVENLPKASL
                     QELLETSYQTNFLGSLVSAYQGWRGIEPPAPNPKRQALAELQRARVSFQPWPDKSGII
                     TISAEASHPTVSVDIINTYIEALMSRTRTFNIDDARVSREFLEQQLTDVKRTMNLSEQ
                     SLQAFVASHGGVRLPDQSKAAADRLGQTEAALAEAVSNRKMVETRLTGLREKVEGQKR
                     PGDKAGSPAPRLSSPEVERLRNELAKLESSLLELRMKYTEEHPRIQLVKNRIDELTNS
                     LGGALKDSVPSTPLASAVPAAERVNFSEQLIGLEATYHSMVAREEALTKQSEILRQSL
                     RGLSGGEAEYARLQREVDSQRSLHAMLSDKLTAARIREQGEMKVVKVIDPPGPGVPAG
                     NEKRLLALAVAFAAALGLGAAVPTGVEWLHRKIENEDDVELATGLPVLALVPSIRTGR
                     PVFMSGIPDTTGRGPSDQLMFTEAFRALRVAIELSTRGEPLKRLLVTSAFAGDGKSTV
                     VANLGYALSEAGHRVVLADTDFLRPTLHRTMKIKSSSGLVETLESETPLEPTLVPVTQ
                     GLWLAQRGESLHPRSRGLLAGSKLRGLIGEMSSRADFVICDSSPVLLIPDNLLLASAV
                     DGVVLVAKAGVTGFRDLAKTK"
     gene            complement(2163..>2633)
                     /locus_tag="VFB89_03415"
     CDS             complement(2163..>2633)
                     /locus_tag="VFB89_03415"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HZM26378.1"
                     /translation="PVLFTQQRVGERGRIFVLKKFRSMSVNAEANGPVWAAARDPRVT
                     RVGRLLRRTRLDELPQFWNVLAGDMSFVGPRPERPEFVATLQREIPFYKGRHSVRPGI
                     TGWAQVRHRYAASIEDSMEKLQYDLYYIKNLSPLLDLVILLSTLQVVLFTRGSR"
BASE COUNT          448 a          851 c          892 g          442 t
ORIGIN      
        1 tcttggtttt cgccaggtcg cggaaccccg tcacgccggc cttcgcgacg aggacgacac
       61 catccacggc ggaggccagc agcaggttgt cggggatcag cagcaccggc gacgagtcgc
      121 agatgacaaa gtcggcacgg ctcgacatct cgccgatgag ccctcggagc ttcgagcccg
      181 ccagcagccc ccgcgagcgc gggtggagcg actccccgcg ctgcgcgagc cataggccct
      241 gggtcaccgg gacgagcgtc ggctccaacg gagtctcgga ttcgagggtc tccaccaagc
      301 cgctgctgct cttgatcttc atcgttcggt gcaaggtcgg tctgagaaaa tcggtatcgg
      361 cgagcaccac acgatgtccg gcctcgctga gcgcatagcc gaggttggcc accacggtgg
      421 acttgccgtc gccggcaaag gcgctcgtca cgagcaggcg cttgaggggc tcaccgcggg
      481 tactgagctc gatcgccacg cgaagcgcgc ggaacgcttc ggtgaacatg agctgatcgc
      541 tggggccgcg gccggtggtg tcggggatac cactcatgaa tacgggacgt cctgtccgaa
      601 tgctcgggac gagagccagg acgggcagtc cggtggcgag ctcaacgtcg tcctcgttct
      661 cgatcttccg gtggagccac tcgacgccgg tcggcacagc cgcgccaagg cccaacgcgg
      721 cagcgaacgc cacggcgaga gccagcaagc gcttctcgtt gccggccgga acaccgggac
      781 cgggcgggtc gatgaccttg accaccttca tctcgccctg ctcgcggatg cgcgcggccg
      841 tcagcttgtc ggagagcatg gcgtgcaggc tgcgttgact gtcaacctcg cgctgcaggc
      901 gtgcatactc ggcctcgccg ccggatagcc ccctcaggct ctgccgcaga atctcgctct
      961 gcttcgtgag ggcctcctcc cgcgccacca tcgagtgata cgtcgcctcg aggccgatga
     1021 gctgctcgga gaagttgacg cgctcggccg ccggcacggc ggaggccagc ggtgtggacg
     1081 gcacgctgtc cttcagtgcg ccgccgagcg agttcgtcaa ctcgtcgatg cggttcttga
     1141 ccagctggat gcggggatgc tcctcggtgt acttcatgcg gagctcgagc agcgacgact
     1201 cgagcttggc aagctcgtta cgcagccgct cgacttcggg cgagctcagc cggggtgccg
     1261 ggctacccgc cttgtcgcca ggccgctttt gaccctcgac cttttcgcgg aggcccgtga
     1321 gacgggtctc gaccatcttg cggttgctga cggcctcggc gagcgcggct tcggtctggc
     1381 caagccgatc ggcagcggcc ttcgactgat cggggaggcg gacgccaccg tgggaagcga
     1441 cgaacgcttg gagcgactgc tcactgaggt tcatcgtgcg cttgacgtcg gtcagctgct
     1501 gctcgaggaa ctcccgggat acccgagcgt catcgatgtt gaaggtgcgc gttcgggaca
     1561 tgagcgcttc gatgtaggtg ttgatgatgt cgacggagac cgtcggatgc gacgcctccg
     1621 ccgagatggt gatgatgccg ctcttgtcgg gccatggctg gaacgacacg cgagcccgct
     1681 gaagctcggc cagggcctgg cgctttgggt tcggggccgg aggctcaatg ccacgccagc
     1741 cctggtaggc gctgaccagc gagcccagga agttcgtctg gtaggaagtc tcgagcagtt
     1801 cctgcagcga ggccttcggc aggttctcca ccaccgtctc ggcgaggctc cgggaggcca
     1861 ggatcgccat ctgcacaggg aagggacggt taggggacat ctcctggaag accgggaccc
     1921 cccggtcggg ccgggcctcg agcagcacca cggcggtcgc acggtagcgc ggtggctcct
     1981 tggacgcgat gaaggccacc ggcagcatca ccaccatggc gatgagactc gcgatgagga
     2041 aaagccgctt gcgacgtcgg aggacgcctc ggaggagacg ctcgacactg gatcctgacc
     2101 acgagcccat gagattcgct ttctctagcc cttgaccgac gccggtattc cggcgagcgc
     2161 catcagcggg agcccctcgt gaagagtacc acctgcaggg tactaaggag gatgaccaaa
     2221 tccaggaggg gcgacagatt cttgatgtag tagagatcgt actgaagctt ctccatcgag
     2281 tcttcgatcg acgccgcgta ccggtgacgc acctgcgccc atccggtgat cccgggccgc
     2341 accgagtgcc gtcccttgta gaagggaatc tcccgctgca gcgttgccac gaattccggc
     2401 cgctccggcc gagggccgac gaagctcatg tctccggcca ggacgttcca gaattgcggc
     2461 agctcatcga gacgcgtgcg ccggagcaag cgaccgaccc gggtgacccg aggatcgcgg
     2521 gctgcggccc acaccggacc gttggcctcg gcgttcacgg acatcgaccg gaactttttg
     2581 aggacgaaga tccgtccgcg ctcaccgacg cgctgctgcg tgaacaggac cgg
//
LOCUS       DASRJQ010000151         7977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3839002, whole genome shotgun sequence.
ACCESSION   DASRJQ010000151 DASRJQ010000000
VERSION     DASRJQ010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7977
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3839002"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VFB89_03420"
     CDS             <1..814
                     /locus_tag="VFB89_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZM26379.1"
                     /translation="ARMMHDRSPRAKAPFVEVNCAGLNATFLDSELFGHEKGAFTDAK
                     DRKQGLFEIADRGTIFLDEIGDLAPELQPKLLKVLETKTFRRLGGTREITVDVRLVAA
                     TNRNLQSEVEGGRFREDLYYRLSVMPLQLPAVRDRSREDRLALITRLGSDLRAELPEA
                     PATFSSEVVERLLAYGWPGNVREMRNVLERAMILGRGQPAISVEHLPGEFRARPGVGD
                     RRHTPLSLDDLERQHIERTLKHHGGNRTRAAQELGISRATLINKIKRYSISA"
     gene            988..1797
                     /locus_tag="VFB89_03425"
     CDS             988..1797
                     /locus_tag="VFB89_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HZM26380.1"
                     /translation="MRREARPGAYLTAVVALAAVTPLLGEPSLRTEQVSQLVVGDTGE
                     VLERKAEWRRVRLDRDGYEGWIHRGFVREWTKRESDAWNDRARGWSEGAELDLSGRRV
                     RLPLRSRVALVPDGVLLPDGRAGRVVRGSVRRFSEVLAESRSMSPEEWAARRFIGAPY
                     QWGGVTPWGVDCSGLVQTAFAARDHSLPRDAAQQALEGKEILPRDIQPGDLLFFRGEM
                     SSGITHVAFAGAGETLVHSTVACGGVLRESWRPGTRAAGLRERLVVVRRIG"
     gene            1842..2540
                     /locus_tag="VFB89_03430"
     CDS             1842..2540
                     /locus_tag="VFB89_03430"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HZM26381.1"
                     /translation="MTLRRLILFDIDGTILLTSGAGRRAITAALAEVVGASDAFGRIR
                     FDGKTDPQIVTEMLASAGDPGPHPPERVRSLCERYVTLLERELAVPVGVTLMPGFPRI
                     LDRLEAEGDVMLGLLTGNLERGAALKLRAAGLDPGRFKVGAYGSDSPERPDLPPIAAR
                     RAEPFFGRPLREGEVVIIGDTPADIACGAGIRARAIGVATGSYSVAQLEACNPHAVFA
                     DLADTERALEAILA"
     gene            2542..3141
                     /locus_tag="VFB89_03435"
     CDS             2542..3141
                     /locus_tag="VFB89_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26382.1"
                     /translation="MAQELELKTVVPEPAALRARLLAAGATLIFRGVMEDRRYDRDSA
                     LLARDEVLRIRRFIEAGGASEAELTWKGRTRRSADGYKLREELSCRIAVGAETVGSII
                     EALGYRVSQAIDRYVELYRLAEAVLRVEWYPRMDVLLEVEGAPAAIEAAVVATGLPRD
                     SFTAEMLADFVQRYDARGGKSAVLAVADLAGEPPAWEAV"
     gene            3138..3890
                     /locus_tag="VFB89_03440"
     CDS             3138..3890
                     /locus_tag="VFB89_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26383.1"
                     /translation="MSAQPRVTSRGLVPELGPALGRLCNRAERQAGRWVNLDDIRLEL
                     ATRIFELAGAARAFASADDRPAAVSSLNRQAWLTEWERAVSASTERIVQAVNLRFDSA
                     GLEARLRTRRRKQLLLAEPDRFAISGRLGAGSLPFLSALDELEQVVPSASAPGARGEA
                     GVPEWQDRILSAARRLESAWLALESAARREETLWSPEISRVRSWRRPTWPVWLVTGLV
                     LGLATYLGLILGGYLLVPAPLLPLAQEVWKRF"
     gene            3957..6095
                     /locus_tag="VFB89_03445"
     CDS             3957..6095
                     /locus_tag="VFB89_03445"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HZM26384.1"
                     /translation="MILIGGIARTLLTLALLAPGQQPASDQAPALRRLAATAQLGAQE
                     YRAGVQNGRVVAKAEIEEARLFFAEARRSAGFLSPEVSSSAVRALDEIADLVDATASP
                     DTIQARVLALTNSLSQRLGISLEEIPAGTPSLARGARIYRENCASCHGDLGRGDGPMA
                     EGLDPKPANLSDAAALSDASPLDFYRRVTIGVVGTSMPAFEGRLSADDRWAAAAYATL
                     LRLPAPKGEVPPSLRRFDGTARLTDPDIRKQLAAAGADTAATLVAAVRSIQEQADAAA
                     TARIFAQVRSQLDSVDHLARAGREAEASTAAFNAYMTFEQVERSVRAKHPDLAGSLEN
                     RFAALRTRAAGGATTMELDGIQRALAADLENAERVLGETLSPFNLFLQSFVILVREGL
                     EAILVLGALMAFLVKTGAASRKRDIHVGVGAAVAASLLTAFALETVFRLSPARREALE
                     GLTMVAATGVLFYVSYWLLSKMEVAKWNRFVRGKVQDAVSSGSALALASAAFLAVYRE
                     GFETVLFYKALFVAGGSGGSTVAVLAGIALGAVVMLFVYVAINRFGVRLPLKPFFGVT
                     SAFLYYMAFVFAGKGVAELQEGNLVPTTIVSSAPRIPALGIYPTVESLTAQAVLLVLL
                     VIALVWTFLIEPRRLKVTSVMVPEPAREAPSAPSRAAAPGVVPVGTRQELLRSLERME
                     ADLAELRAEVERMRARLRARDSQTVPRNNP"
     gene            6164..6490
                     /locus_tag="VFB89_03450"
     CDS             6164..6490
                     /locus_tag="VFB89_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26385.1"
                     /translation="MIARTWHGMVPATRADEYKAYLERTGLPDLRATPGNRGVWVLRR
                     TEGDRTHFLLVSLWDSMGSIRAFAGDDVERARYYPEDKDFLLELEPHVTHYDVIARPA
                     AAEVRG"
     gene            6487..6969
                     /locus_tag="VFB89_03455"
     CDS             6487..6969
                     /locus_tag="VFB89_03455"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZM26386.1"
                     /translation="MTETERIVDELRRAQDRGAWHGPSAREGLEGITAAAAASHPIPG
                     AHSIWELVLHLTAWRGEVMRRLQGAEPGLPPEGDWPPVGDTSEAAWAEARRRLEDSHR
                     QLGEAVARFAEEKLDQPVGRKPPDPALGNVGTYYVMLHGVAQHDAYHMGQVGILKKVV
                     "
     gene            complement(7281..>7977)
                     /locus_tag="VFB89_03460"
     CDS             complement(7281..>7977)
                     /locus_tag="VFB89_03460"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HZM26387.1"
                     /translation="GIVGAKLWYVALFRDPSALFSRGGLVWYGGFIGGVLAVLANGAR
                     LKVPTRWTMQLGAPALAAAYALGRVGCFLVNDDYGRPTSLPWAVRFPKGLPPSTAANL
                     HQQFGIPIPPGTDPSTVLAVHPTQLYEVAIMLVAFAILWALRTRARATGWLFGLYLVL
                     AGIERFLVEILRAKDDRVLWGGFTLAQLTSILLVLIGAALLARWRGDVEVPAGPYLAP
                     DNRETSAKTFAPA"
BASE COUNT         1346 a         2552 c         2662 g         1417 t
ORIGIN      
        1 cgctcggatg atgcacgatc ggagcccgag ggccaaggcg ccgttcgtgg aagtgaactg
       61 tgccggcctc aacgcgacct ttctcgactc cgagctgttt ggtcacgaga agggtgcatt
      121 caccgacgca aaggaccgca agcagggact cttcgagatt gcagaccgtg gtacgatttt
      181 tctcgacgag atcggtgacc tggcgcccga gttgcagccc aagctcctca aggttctcga
      241 gacgaaaacc tttcgtcggc tgggcggcac gcgtgagatc acggtggacg tccggctggt
      301 cgccgcgacc aaccggaatc tgcaaagcga agtcgagggc ggccggttcc gcgaggatct
      361 gtactatcgc ctcagcgtca tgccgcttca gcttcccgca gtgcgggacc gctcccgcga
      421 ggatcgtctc gccttgatca cccggttggg gagcgatctc cgggccgaac tgccggaggc
      481 cccggccacg ttttccagcg aggtagtcga gcgcttgctg gcctatggct ggccgggcaa
      541 tgtgcgtgag atgcgcaatg tcctcgagcg cgccatgatc ctcggccgcg gccaacccgc
      601 gatcagcgtc gagcatctcc cgggcgagtt ccgggcccgc ccgggagttg gcgatcggag
      661 acacactccg ctcagtctcg acgatctcga acggcagcac atcgagcgta ccttgaagca
      721 tcatggcggc aaccggacca gggccgcgca ggagcttgga atctcccgcg ccactctcat
      781 caacaagatc aagcggtata gcatcagtgc ttgagtcgaa cgccccgctg agccgatgag
      841 cgagcactac catcccaggg aaaaggacgc gatgcattgt cgcgcctgcg gccgtgagga
      901 acgggcttcc gaagggtatc cctgcgtcga gtgcggcacg ttcatctgcc tcatctgtac
      961 tttccgcggc gtcaatcatt gctccgagtg cgccgcgaag ctcggccggg cgcctacctg
     1021 acggccgtcg tcgccttagc cgccgtcacc ccgttactcg gcgagcccag ccttcgaacc
     1081 gagcaggtat cgcagttggt cgtgggcgac accggcgagg tactcgagcg caaggcagag
     1141 tggcgtcgag tgcgcctcga ccgcgacggc tatgaaggct ggatccaccg cgggttcgtg
     1201 cgggaatgga ctaagcggga aagcgatgcg tggaacgatc gcgcgcgggg ctggagcgaa
     1261 ggtgccgagc tcgatctgag cggtcgtcgg gtgcgacttc ccctccgctc ccgagtggcc
     1321 ctggtgcccg acggcgtgct gctgccggac ggacgcgctg ggcgcgtggt gcggggctcg
     1381 gtgcgacgat tttccgaggt tctggccgag tcgcgctcga tgtctccgga ggaatgggcc
     1441 gcgcgtcggt tcatcggggc gccgtaccaa tggggtgggg tcactccctg gggtgtcgac
     1501 tgttcgggcc tggtgcagac ggccttcgcg gcgcgggacc actccctgcc gcgcgacgct
     1561 gcgcaacagg cccttgaagg caaggaaatc ctcccacgcg atatccagcc aggcgacctg
     1621 cttttcttcc ggggcgagat gagcagcggc atcactcacg tggcattcgc cggggccggt
     1681 gagaccctgg tgcactcgac ggtcgcgtgt ggcggcgtgc tgcgcgagtc ctggcgacct
     1741 ggaacacggg ctgccggact ccgcgagcgg ctggtggtgg tccgccgcat cggctgagca
     1801 cggtcagccc agttcggcgc ccacccctct attttcccgc gatgaccctg cgtcgactga
     1861 ttctcttcga catcgacggc acgattctgc tgacctccgg cgcgggccgg cgcgccatta
     1921 cggccgcgct ggccgaagtg gtgggcgcga gcgacgcatt cggacggatc cgcttcgacg
     1981 ggaagaccga tcctcagatc gttaccgaga tgttggcatc ggccggagac cccggaccgc
     2041 atccgcccga gcgggttcga agcctttgtg agcgctacgt cacccttctg gaacgagagc
     2101 tcgccgtacc tgtgggcgtc acgctcatgc ccggctttcc ccggatcctc gaccgcctgg
     2161 aagcggaagg ggatgtgatg ctcggccttc tcacgggcaa tctcgagcgc ggcgcagcgc
     2221 tcaagctgag agcggccgga ctcgatccag gccggttcaa agtcggcgcc tacggctccg
     2281 actcgccgga gcgacccgat ctgcctccaa ttgcggcccg ccgtgccgag ccgttcttcg
     2341 gacggccgct acgcgagggc gaagtggtga tcatcggcga cacccccgcg gacatcgcct
     2401 gcggtgcggg catccgggct cgagctatcg gtgtagcgac tggctcgtat tcggttgccc
     2461 agctcgaagc ctgcaatccc cacgctgtct tcgccgacct ggctgacacc gagcgcgctc
     2521 tcgaagcaat cctcgcctga catggctcag gagctcgagc tcaagaccgt tgtgcccgag
     2581 cctgccgcgc tcagggctcg cctgctcgcg gccggggcga cgctgatctt tcgtggcgtc
     2641 atggaagacc gccgctacga tcgcgacagc gcgctgctcg cgcgcgacga agttctccgg
     2701 attcgtcgat tcatcgaagc gggcggagcg tccgaggctg agctgacctg gaaaggtcgc
     2761 acccgccgat cggcggacgg gtacaagctg cgcgaggagc tgagctgccg aatcgccgtc
     2821 ggtgccgaga ccgtcggctc gatcatcgag gcactggggt accgggtcag ccaggcgatc
     2881 gatcggtatg tcgagctgta tcggctagcc gaagccgtgc tccgggtcga gtggtatccc
     2941 cggatggacg tgttgctcga ggtggaaggg gcgcccgctg caatcgaggc cgccgtcgtc
     3001 gcgaccggac ttcctcgaga cagcttcacg gccgagatgc tggccgattt cgtacagcgg
     3061 tacgacgcgc ggggcggaaa gagcgccgtt ctggcggtgg ccgacctggc cggcgaaccg
     3121 ccggcctggg aggcggtgtg agcgcccagc ctcgggtcac ctcgcgcggg ctggttccgg
     3181 aactcggtcc ggcgctcggc cgactgtgca atcgggctga gcgtcaagcc ggccgctggg
     3241 tcaacttgga cgacatccgc ctcgagcttg cgacccggat cttcgagctc gccggggcag
     3301 ctcgcgcctt tgcatcggcg gacgatcgcc ctgccgcggt gagctcgctc aatcgtcagg
     3361 cctggctcac tgaatgggaa cgcgcagtaa gcgcgagcac cgagcgcatc gtccaggccg
     3421 tcaatctccg attcgactcg gccggcctcg aggcccggct ccgcacgcgc cggcggaagc
     3481 aactgcttct tgcggagccg gatcgctttg ccatatccgg tcgattgggc gccggcagcc
     3541 tcccgttcct tagtgccctc gacgagctcg agcaggtggt gccgagcgca tcggccccag
     3601 gtgcgcgggg agaggcaggg gtcccggagt ggcaggacag gattctgtcg gccgcccgcc
     3661 ggttggaatc ggcctggctt gcgctcgaat cagccgcccg gcgcgaggaa accctttgga
     3721 gcccggaaat cagccgggtt cgatcctgga ggcgtccaac ctggccggta tggctggtca
     3781 ccggtctggt gctcggcctg gccacctatc tcggtctaat tctggggggg tatctcctcg
     3841 ttccggcccc gctactacca ctggcccagg aggtttggaa acgattctga catcatccct
     3901 tccaattgcg attggcgcac tctagtatct agcggcacct ccaaatcttt ttgaaaatga
     3961 ttctcatcgg tggcatcgct cggactcttc tgaccttggc cctgttggcg ccggggcagc
     4021 agcctgccag cgatcaagct cccgcgcttc gccggctcgc tgcaacggcg cagcttggcg
     4081 cgcaggaata tcgagccggt gttcagaacg gccgcgtggt cgcgaaagcc gaaatcgagg
     4141 aagcccggct gttcttcgcc gaagctcgcc gaagcgccgg attcctctcg ccagaggtca
     4201 gcagctcggc ggtccgagcg ctcgatgaga ttgccgacct ggtggatgcc accgcgagcc
     4261 ccgacacgat ccaggcccga gtccttgcgc tcaccaactc attgtctcag cggcttggta
     4321 tttcgctcga agagattcct gcggggacgc cctctctcgc gcgaggcgcc cggatctatc
     4381 gggagaattg cgcgagctgc cacggcgacc tgggccgagg agacggcccc atggccgagg
     4441 gattggatcc caagcccgcc aacctttcgg atgccgcggc gctgagcgat gcgtctccgc
     4501 tcgattttta tcgccgggtc accatcggcg tcgtgggaac ctcgatgccg gccttcgagg
     4561 gtcgcctgtc cgccgacgat cgctgggcgg ccgcggcata cgccacgttg ctccggcttc
     4621 cggcccccaa gggagaagtg ccgccctccc tgcgtcggtt cgacggaact gcccgcctca
     4681 ccgaccccga catcaggaaa cagctggccg ccgctggagc cgacacggca gctacgctgg
     4741 tcgctgcggt gcgcagtatc caggagcagg cggatgcagc agcgacggca cgcattttcg
     4801 cccaggttcg aagccaactg gattcagtcg atcatctcgc ccgcgcgggc cgggaggcgg
     4861 aagcaagtac ggccgcgttc aacgcgtaca tgacgttcga gcaggtcgaa cggtcggtca
     4921 gggcaaaaca tcccgatctc gccggctcgc tcgagaatcg cttcgccgcc ctccgaacca
     4981 gagccgctgg cggagccact accatggagc tggatggcat ccagcgtgcc ctcgcggccg
     5041 atctcgagaa cgccgagcgg gtgctgggtg agacgttgtc gccgttcaac ctgttcctgc
     5101 aatcgttcgt gatcctggtg cgggaaggcc tcgaggccat tctcgtactc ggcgcgctga
     5161 tggcattttt ggtaaagacc ggcgccgcga gtcgcaagcg ggacattcac gtgggcgttg
     5221 gagcggcggt ggccgcaagc ctcctcacgg cctttgccct cgagacggtg ttccggctga
     5281 gcccggctcg gcgtgaggcg ctcgaggggc tcacgatggt ggccgccacg ggagtcctgt
     5341 tctacgtgag ctactggctg ctttcgaaga tggaagtagc caagtggaac cgcttcgtac
     5401 gcggcaaagt acaggatgcc gtaagcagcg ggtccgcgct ggcgctcgcc tcggcggcgt
     5461 tccttgcagt gtatcgcgaa gggttcgaga cggtcctctt ttacaaggca ttgttcgtag
     5521 cgggggggtc gggcggctcg actgttgccg ttctcgcagg catcgcgctt ggtgcagtcg
     5581 tgatgctgtt tgtctatgtc gcgatcaatc gcttcggggt gcggctgccg ctcaagccct
     5641 tcttcggcgt gaccagcgcg tttctctact acatggcctt tgtgttcgcc gggaagggag
     5701 tggccgaact gcaggagggc aacctcgtac ccaccacgat cgtctcctcg gctccgcgga
     5761 tcccggcgct ggggatctat cctacggttg agtcgcttac agcccaggcc gtcctgctcg
     5821 ttctgctggt gatcgcgctg gtgtggacgt tcctgatcga gccgcggcgg ctcaaggtca
     5881 cctccgtgat ggttccggag ccggcacggg aggcgccatc agctccctca cgagcggctg
     5941 cgcccggtgt ggtgccggtc gggaccagac aggagctgct tcgctcgctc gagcggatgg
     6001 aggccgatct cgcggagttg agggccgagg tggagcgcat gcgggcgcgc ctcagggccc
     6061 gcgactcaca gactgttccg cgcaacaatc cgtaacgctg ccaccatgct cggtcgaaac
     6121 ggcccgcccc cgcgggccgt atgcgttggg acaggaggca atcatgatcg cgagaacctg
     6181 gcacgggatg gtgcccgcaa cgcgggccga cgagtacaaa gcgtaccttg agcgcaccgg
     6241 actgcccgat cttcgagcga cgccgggcaa tcgtggcgtc tgggtgctgc gtcgaaccga
     6301 aggcgatcga acccactttc tgctcgtgtc gctttgggac tcaatgggtt ccattcgtgc
     6361 gtttgccggc gacgacgtcg aacgggctcg ctattatccg gaagacaagg acttcttact
     6421 cgagctggag ccgcatgtca cccactacga tgtcatagct cggcccgctg cagcggaggt
     6481 acgcggatga ccgaaaccga acggatcgtg gacgaacttc gccgcgcgca ggatcggggc
     6541 gcctggcacg gaccgtcggc acgcgaaggc ctggagggca tcaccgcggc ggctgcggcg
     6601 agccacccga tccccggtgc ccactctatc tgggagctgg ttctgcacct cacggcttgg
     6661 cggggtgaag tcatgcgacg attgcaaggc gccgagcctg ggttgccgcc tgaaggggac
     6721 tggccgccgg ttggggacac cagcgaggca gcctgggccg aggctcgccg tcggctggag
     6781 gactcgcaca ggcagctggg agaggcggtc gctcgattcg cggaggagaa gctcgaccaa
     6841 ccggtagggc gtaagccgcc cgaccccgcg ctgggtaatg tcgggacgta ttacgtgatg
     6901 ctgcatggtg tggcccaaca cgatgcgtac cacatgggcc aagtgggcat tctgaaaaag
     6961 gtggtctgat gcaaaaggaa acggtccgcc gttgtcggcg gaccgttcgt aagtgaggtg
     7021 gcagatcgcc ccctgagttt tcggaaacgg accttagtgt ccgctccacc cttcttcgac
     7081 ctgccacttt tccgtgggag caaaatcgct ccgtcacgct cttgaaaaag caaggcaggt
     7141 gccagttgtg catcaaagag tgaacagctt ggaaaactgt gacttagctg caaaaggggc
     7201 ggcgactcgg ccgccttttt tctgtcagca atcttgacac aacccggaca gtaattcgaa
     7261 agtccagcta ctccataggc ttaggcgggc gcaaaagtct ttgccgacgt ttcccgattg
     7321 tcaggcgcca aatacgggcc cgcgggcacc tcgacgtcgc cccgccagcg cgccagcaag
     7381 gcagccccga tcagcaccag cagaatgctg gtaagctgag ccagcgtgaa accaccccac
     7441 agaactcgat cgtctttggc ccgcagtatc tcgacaagaa agcgctcaat tcccgccagt
     7501 acgagataaa ggccgaacag ccagcccgtc gcgcgggccc gggtccgtaa tgcccaaagg
     7561 attgcaaagg ccaccagcat gatcgccact tcatagagct gagtggggtg tacagccagc
     7621 acggtgctgg gatcggtgcc cggcgggatc gggatcccga actgctggtg caggttcgcg
     7681 gccgtagacg gcggcaagcc tttgggaaac cgtacggccc atgggaggga agtgggccgg
     7741 ccataatcgt cgtttaccag gaaacaccct actcgtccca gcgcgtacgc tgcagcaagc
     7801 gccggggcgc ccagctgcat ggtccatcgg gtcgggacct tgagtcgcgc gccgtttgca
     7861 agaaccgcca gaactccgcc gatgaagccg ccgtaccaaa cgagtccgcc gcgagagaac
     7921 agggcgctgg gatcgcgaaa gagcgcgacg taccagagtt ttgctcccac gattcca
//
LOCUS       DASRJQ010000152         3595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2715076, whole genome shotgun sequence.
ACCESSION   DASRJQ010000152 DASRJQ010000000
VERSION     DASRJQ010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2715076"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1658)
                     /locus_tag="VFB89_03465"
     CDS             complement(<1..1658)
                     /locus_tag="VFB89_03465"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM26388.1"
                     /translation="MTPYKPQETFGKNLRHGLVESEALIASLERVRRAHAGVKKNVVP
                     SITTFYSHEDSPIRSTALLFTAALLAERGQRVLLVDWNLESPGIARDAQGAASTPGLV
                     ELLRSFTEPQKLSWRDCVFPVRSFLGSTELHVLSAGRDDVDYEARFRGLNVAQLVERH
                     DLGRRLEAIRAEWLGAYDAILIDSPPGTGLLSGLCVIHLADRVVLLLAPDKKSISVAK
                     SILRRATAQQMRLPLDRKRLIAIPVLAVETATALDASWRELAAQELGEFLADWLPRSA
                     RPVDAIPSLAVGANPSSSDFLSDYNRLARLLDGDMTELAQTNPGGVRPFPLQRSSRYR
                     PLKWIKDGAMGSVWRAYNCNLGIEVALKFVRGGDYERTKAILAEGRNMARVDHDRVVK
                     VLEVGEAEGQAFIAMQYVDGETLGQKAPHLTIEQKVLLLRDVAFGVQAVHEAGLIHRD
                     IKPSSILVKDSSEGLKPYLMGFGVARKEKVAANETRELIGTPEYMSPEQVRGYVASLD
                     RRADVYGLGATLYALLVGRPPFQDEDELRLLHRVLREKPVSPRER"
     gene            complement(1661..2263)
                     /locus_tag="VFB89_03470"
     CDS             complement(1661..2263)
                     /locus_tag="VFB89_03470"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HZM26389.1"
                     /translation="MPYQYEIFLSYPRLPMVVKWLDKFFEPLFSQWLREELLAQDPRM
                     KLRIFRDRDIGAGEQWPRIIREAARDSRCAVAILMPSYFASDWCRAEWATFRLRGKEH
                     SRNLLVPVRFHDCLHMSLAEQVFDLSQYTAMAEDTPRWVDFNDQVKDLAAQVAQRVLS
                     APPYPADPECARWLALEVPDSPSLPPILPSAFDLNDTPED"
     gene            2656..>3595
                     /locus_tag="VFB89_03475"
     CDS             2656..>3595
                     /locus_tag="VFB89_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26390.1"
                     /translation="MSPRPLTKSLAGALVALALAVPAGAAPALASWGSLPVSFEPNRG
                     QTHPSVDFIARSRDGVGFLAGGALTLRLDRCARARDCRTHAVRMRLAGAHPRKPEGLK
                     PLPGIASYFLGSDPSRWIAGVPTYGQVLYRDALPGVDLRYHSSEGHLELDLVLAPGAS
                     PGPLRLAFDGADRVAVDPNGDLQLAAAGQPLTLRRPVAYQEIAGERRPVAAEYVVKGT
                     RAVTIALGERDRSRPVVVDPVLVYSAQLGGPSNDGLEAVAVDSSGAAYAAGYAGSGFP
                     TGAGSQPHGGGVDAVVLKLDPSGSALVYSVYLGGGFA"
BASE COUNT          639 a         1193 c         1133 g          630 t
ORIGIN      
        1 cgttcacgcg gggagacggg tttctcccgg agcactcgat gtagcaaccg cagttcgtct
       61 tcgtcctgga agggaggccg accaacgagc agggcataca acgttgctcc caagccgtag
      121 acgtccgcgc gacggtccag cgaggcgacg tagccgcgca cctgttctgg gctcatgtat
      181 tcaggcgtgc cgatcaactc cctggtctcg ttggcggcca ccttctcttt ccgggccaca
      241 ccgaagccca tcaggtaggg cttcagtccc tcgctcgaat ccttcaccaa gatgctggag
      301 ggcttgatgt cccggtgaat gagcccggct tcatgcaccg cttgaacgcc gaacgcgacg
      361 tctcgaagga ggagtacctt ctgctcgatg gtgaggtgag gcgccttctg accaagggtc
      421 tcgccgtcca cgtactgcat cgcgatgaag gcctgcccct ccgcctcacc aacctcgagc
      481 accttcacga cacggtcgtg atcgacccgc gccatgttcc tgccctcggc gaggatggct
      541 ttggtgcgct cgtagtcacc tccgcggaca aacttgagcg cgacctcgat tcccaggttg
      601 cagttgtagg cgcgccagac gctgcccatc gcgccgtcct tgatccattt cagcgggcga
      661 taccttgatg atctctggag gggaaatggc cggactccgc ctggatttgt ctgggccaac
      721 tcggtcatgt caccatcgag cagcctggcc agcctgttgt agtcagagag gaaatcggag
      781 gagcttgggt tagccccgac ggcaagggac ggaatggcgt caacaggcct ggccgatcga
      841 ggcagccaat cagcgaggaa ctcaccaagc tcttgtgccg ccaactctct ccacgaagca
      901 tcgagagcag tggccgtctc gaccgccaac accggaatcg caatcagcct cttccgatcc
      961 aagggtagcc gcatttgctg tgcggtcgcg cgccgcaaaa tcgacttggc cacactgatg
     1021 ctcttcttgt cgggcgcaag cagcagtacc accctgtcgg ccagatggat gacacagagg
     1081 ccactgagaa gaccggtacc cggaggacta tcgatcagaa tcgcatcgta tgcaccaagc
     1141 cactcggcac gtatggcctc cagcctccga ccgaggtcat gccgttcaac caattgagcg
     1201 acattcaaac ctcggaaccg tgcttcatag tcgacatcat cccggcctgc ggaaagcacg
     1261 tgcaactctg tcgaacccag gaacgagcgc accgggaaca cgcagtctcg ccatgacagc
     1321 ttctgaggtt ccgtgaatga gcgcaggagc tctacgagcc cgggcgtaga cgctgccccc
     1381 tgggcatcac gcgcaattcc tggcgactca agattccagt ccaccagcag cacccgctgt
     1441 ccgcgttcgg ccagcagagc tgcggtgaac agcagcgctg tcgagcggat cgggctgtct
     1501 tcatgtgagt agaatgtggt gatcgacgga acgacgttct tcttgacgcc agcgtgcgct
     1561 cgacgcacac gctcgaggct tgcgataagc gcttctgact ctaccaagcc atggcgtaga
     1621 ttcttaccga acgtctcctg gggcttgtac ggggtcatgc tcagtcctca ggcgtgtcgt
     1681 tcagatcgaa ggcactcgga aggatgggag ggagggacgg cgaatcagga acctcgagcg
     1741 cgagccagcg tgcgcattca ggatcggctg gataaggagg agcgctcaga actcgctgcg
     1801 ccacctgtgc cgccaagtcc ttcacttgat cgttgaagtc cacccagcgt ggcgtgtctt
     1861 cggccattgc agtgtattga ctcagatcga agacctgctc cgccaaagac atatgaaggc
     1921 agtcatggaa gcgaaccggc acgagaaggt ttcggctgtg ctcctttcct ctgaggcgga
     1981 atgttgccca ctcggcccgg caccagtctg aggcgaagta tgagggcatc aggatcgcca
     2041 ctgcgcaacg cgagtcgcgt gccgcctccc gaatgatccg cggccactgc tcgccggctc
     2101 cgatgtccct gtcgcggaag atccggagct tcattcttgg atcctgcgcg aggagctctt
     2161 cgcgcagcca ctggctgaag agcggctcga agaacttgtc gagccacttc accaccatgg
     2221 ggagacgggg atagctcagg aagatctcat actggtacgg catcagaaga ccccaccgac
     2281 ggagaagggc attcataccg atcaggcatg gagtcaatcg acagatcagc ctccgcggac
     2341 acgctccggc agggggtcgg cagcccccag tggtcggcat ccatctgctc gacattagga
     2401 acgcggacca tccacttcat tacctccggc caccgcgcag tgtcaggctc agagtgtagg
     2461 tttcacgagg tcgccaaggc cgcagacatg gacccagtcc gaactttcga aggcggagcg
     2521 gcgcaaggac catctcctct cgacgctccg ccgctacgtc gaggctctgg ggggcgagat
     2581 cgaagtcgtc gcccactttg gagacaagcg catccggcta caaggagtct gacgactgga
     2641 gttagccttc cgcccgtgag cccaagaccc ctgacaaagt cgctggcggg cgcgctggtc
     2701 gccctcgccc tggctgttcc cgcgggtgcg gcgccggccc tggcgagctg gggctcgctt
     2761 ccggtctcgt tcgagcccaa ccggggccag acccatccct cggtggactt catcgcccgc
     2821 agccgcgacg gggtcggctt cctcgccggg ggcgcgctca cgctgcgcct cgaccggtgc
     2881 gcccgcgccc gcgactgccg cacccacgct gtccgcatgc gcctggcggg cgcccaccca
     2941 cggaagcccg aggggctgaa gccgctgcct ggcatcgcca gctactttct cggctccgac
     3001 ccttcgcgct ggatcgccgg ggtccccacc tacgggcagg tcctctaccg ggacgcgctg
     3061 ccgggcgtcg atctccgcta ccacagctcc gaggggcacc tcgagctgga cctggtcctc
     3121 gccccgggcg cgagcccggg ccccctccgc ctcgccttcg acggcgcgga ccgcgtcgcg
     3181 gtggatccga acggcgacct ccagctcgcg gccgccggcc agccgctcac gctccgccgc
     3241 cctgtcgcct accaggagat cgcgggcgag cgcaggccgg tggccgcgga gtacgtggtg
     3301 aagggaacgc gggcggtgac gatcgcgctg ggcgagcgcg accgttcccg gccggtggtg
     3361 gtggatccgg tgctggtgta ctcggcgcag ctcggcggtc cctcgaatga tgggctggag
     3421 gccgtcgcgg tggactccag tggcgcggcc tatgccgccg gctacgccgg gtccggattc
     3481 ccgaccggcg ccgggagcca gccccacggc ggcggggtcg acgcggtggt gctgaagctc
     3541 gaccccagcg gctccgccct ggtgtactcg gtgtatctcg ggggcgggtt cgcgg
//
LOCUS       DASRJQ010000153         3190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1585451, whole genome shotgun sequence.
ACCESSION   DASRJQ010000153 DASRJQ010000000
VERSION     DASRJQ010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585451"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="VFB89_03480"
     CDS             complement(<1..1012)
                     /locus_tag="VFB89_03480"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM26391.1"
                     /translation="MAAPPPRSGAPAVRATSPVNVSAPPRQSSPTRWSPSPVHAASPA
                     SPPSNRTAPPAQAEKPPPAPRNSDGDVYAWRSSSQERFPYPDGAGFQSPDPFWNPESR
                     GANLVRIRSSLELARSLRDEGEYFGAIQRAKEALRLLADTSNGDRPLQARSLGLLGEL
                     YWLRGDFEPAESNLRNALAMYESGLITGDSDLATLLNKLALVYQSRGQFGQAEPLHKR
                     ALAIQEAALDKDHSDFAASLNNLAILYKAQGLYSRAQSLHERALVIRQNAFGTIHPDV
                     ASSLINLAAVHKAQGQYASAKPLVQRALGVQEATLGADHPDVALSLNSLAAIYEAQGQ
                     YSE"
     gene            1476..2945
                     /locus_tag="VFB89_03485"
     CDS             1476..2945
                     /locus_tag="VFB89_03485"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM26392.1"
                     /translation="MMVTNSDQQLWIETISGWRPPFGIDRYEIRPGRYLLKVRPTWIV
                     PFFSDAKYAVLRLDAKPGHIYKLRRHQGPDRSWIPRIEDVAPSELGRETPPGNELDHA
                     CRTGHVSSCVELGELYERGSMEVGWDERRAAALLRKACASGHVVGCFSLARLYETGHG
                     VTKDEAVAAEMFKSACIAGEARACTRWGDILQGGPSQPRDQRKAQAPEPLKAACDSGA
                     PAACYWVAVFIRENQGGSVDQGLLATLYEKACTGGEPRACAELGLLYESGIGVELSEH
                     RAAALYGKACAGGSAMGCSYLGGLYQLGRGVTKDPDRAIELHKKGCAAGAATACATLE
                     TLDPKASKTHLKDSQKSVQEPSFRPDFEPLWFQLGVRGDWELLQPSFLPMISAELGTD
                     WYSIVTAIVLAPLEIRFEGRWYPMMWGPIRPYLAVGGLSFGGRAESITIGAPLRLALG
                     MDVRISGVQIFTDFAPLRSTAPADMFGPGLLSLGAGWRF"
     gene            complement(2957..>3190)
                     /locus_tag="VFB89_03490"
     CDS             complement(2957..>3190)
                     /locus_tag="VFB89_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26393.1"
                     /translation="EDWLFEAAWRSDGASCIGRTRDGRPLKTVLQECPGRFKPGAEDL
                     GDGDKCVVQSDAAGSPVVLRNRSPVSKRPFLGP"
BASE COUNT          542 a          962 c         1124 g          562 t
ORIGIN      
        1 cttcgctata ttgaccctgt gcctcgtaga tggcggccag gctgttcagc gacagggcga
       61 cgtccgggtg atcggcgccg agggtggcct cctgaacgcc gagcgcgcgt tgaaccagcg
      121 gcttggccga ggcgtactgg ccctgggcct tgtgaactgc ggccaggttg atcagcgacg
      181 aggctacgtc gggatgaatc gtgccgaacg cgttctgccg gatgacgagc gcgcgctcgt
      241 gaagggattg ggcacggcta tacaggccct gagccttgta gaggatggcg aggttgttga
      301 gcgaagcggc gaaatcggaa tggtccttat ccagggccgc ctcctggatg gcgagcgcgc
      361 gcttatggag cggctcggcc tgaccgaact ggcctcgcga ctggtacacc agggcgagtt
      421 tgttgagcag ggtggcgaga tcggaatcac cggtgatgag accgctttcg tacatggcga
      481 gcgcgttgcg caggttcgac tcggcgggct cgaagtctcc ccggagccag tacaactcac
      541 cgaggagacc gaggcttcgg gcctggagag gtcggtcacc gttggaagtg tccgcgagga
      601 gccggagggc ttccttggcg cgctggatgg cgccgaagta ctcgccttca tcccggagcg
      661 atctggcgag ttccagactc gaccgaatgc ggaccagatt cgcccctcgg ctctcggggt
      721 tccagaaagg atcgggcgac tggaaacccg cgccatccgg atacgggaag cgctcctggg
      781 aggacgaacg ccacgcatag acgtcgccgt cggaattccg gggagcgggt ggaggcttct
      841 ccgcctgggc cggcggcgcg gtccggttgc tcggcggcga ggccggcgat gcggcgtgaa
      901 ccggggaagg cgaccagcgt gtgggactcg attggcgcgg aggagcggat acgttgaccg
      961 gcgacgtggc ccgaaccgcg ggcgcgccgc tccgcggagg cggggcagcc accgaccgag
     1021 cacctggctg ggcagggctg gcggtgggtt gctgagcggc gggctgctga ggtgcggtgg
     1081 atgtactggg agacgacggc cggcgctctc gattctctcg tggctccgag ggctcggcgc
     1141 gcggaggacg gttcctcgat ggctccaggg ccacggcgtt cgcggagcat atgaggcaag
     1201 cggagcacag cagcccagcc aaccaaggtg atgaagttcg cgctatcatc tcgaacatgc
     1261 ctccactcgt tccacttggt cctggtgatc caaggaggtc tatccattca agcgtcctgg
     1321 accgaaaaca agacatctta aggcgtgcca ttcgtgtcgt gaggaactcc ttagagttcg
     1381 aggtctacgt cgcgctcctg gcatccgcgt gcctcagcgg atgcactttc gcgagggggt
     1441 actccgggga accacagctg cgcagtgagg ttgccatgat ggtcacgaac tcggaccaac
     1501 agctttggat cgagaccatt agcggctgga ggcccccatt cggcatcgat cgatacgaga
     1561 tccgcccggg gcgatatctc ctcaaggttc gtccgacatg gatcgtgccc tttttctcgg
     1621 acgcgaagta cgccgtgctg cggctggacg ccaagcctgg tcacatctac aagttgcgaa
     1681 ggcaccaggg cccggacagg tcgtggattc cccggatcga agatgtagcg ccgagcgagc
     1741 tgggtcgaga aacgcctccg ggcaatgagc tcgatcacgc gtgtcgcacc ggtcacgtgt
     1801 cgagctgcgt cgagctcggt gagctctacg aacgaggcag catggaagtg ggctgggatg
     1861 agcgacgcgc cgccgcgctc ctcaggaagg cctgcgcatc tggccatgtc gtgggatgtt
     1921 tctcgctggc ccgcttgtac gagaccggtc atggagtgac taaggacgag gctgttgcgg
     1981 cggagatgtt caagagcgct tgcatcgccg gcgaggcacg agcttgtacc cgctggggcg
     2041 acatcctgca gggagggccc tctcagccgc gagatcagcg caaggctcag gctcccgagc
     2101 ctctcaaggc ggcgtgcgac tccggggctc cggcggcttg ttactgggtg gccgtcttca
     2161 tccgggaaaa ccaggggggg agcgtcgacc agggactgct cgccacgttg tacgagaagg
     2221 cttgtacagg tggtgaaccg cgggcctgcg ccgagctcgg gctgctgtac gagagtggga
     2281 tcggagtcga gctgagcgag caccgcgcgg ccgcgcttta cgggaaggcg tgcgcgggtg
     2341 gttcggcaat gggctgctcg tacctgggtg ggctctatca actggggcga ggagtcacca
     2401 aggatccaga ccgcgccatc gagttgcaca agaagggctg cgcggcgggg gcagccacgg
     2461 catgcgccac cctggagacc cttgatccga aggccagcaa aacccatctc aaagactccc
     2521 aaaagtccgt gcaggaacca tctttcaggc cggacttcga gccgctgtgg tttcagcttg
     2581 gcgtgcgcgg tgactgggag ctgcttcaac cgagcttcct tcccatgatc tccgccgagc
     2641 taggaacgga ctggtactcc attgtcacgg ccatcgtcct ggcccccctg gagatccgct
     2701 tcgagggccg ctggtatccg atgatgtggg gaccgattcg gccgtacctc gccgtgggtg
     2761 gcctgagctt cgggggccgc gctgagagca tcacgatcgg cgcccccctg cgcctagcgc
     2821 tcggcatgga cgtgcggata tcaggagtcc aaatcttcac tgatttcgcc ccgcttcgct
     2881 ccacggcgcc agcggacatg ttcggtccgg ggttgctgtc ccttggtgcc gggtggcgct
     2941 tctagaacct cgggtactag ggacccagga atggccgctt gctcaccggc gaacggttcc
     3001 gcagcaccac gggcgacccg gcggcgtccg actggaccac gcacttgtca ccgtcaccca
     3061 ggtcctccgc gcccggcttg aaccgtccgg ggcactcctg gaggacggtc ttgaggggcc
     3121 gtccgtcgcg cgtcctgccg atgcaggaag cgccgtcgga cctccacgcg gcctcgaaga
     3181 gccagtcctc
//
LOCUS       DASRJQ010000154         2999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3040459, whole genome shotgun sequence.
ACCESSION   DASRJQ010000154 DASRJQ010000000
VERSION     DASRJQ010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3040459"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..545)
                     /locus_tag="VFB89_03495"
     CDS             complement(72..545)
                     /locus_tag="VFB89_03495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HZM26394.1"
                     /translation="MPARAFVIGVISDTHGLLRPEAVDALRGSDLIIHAGDVGKPELI
                     DRLEDIAPTHVVRGNIDHGDWAAALPMRELVEVGEHRFFVLHDISQLDADPVASGIAA
                     VVFGHSHRPSIETHNGVLLLNPGSAGPRRFKLPVTIARVRVSGRHLRPEIVELQV"
     gene            complement(631..1023)
                     /locus_tag="VFB89_03500"
     CDS             complement(631..1023)
                     /locus_tag="VFB89_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26395.1"
                     /translation="MSTAFGTRAAAVESPFQVTRNADLGSIEATKAGLVGSYVVTGTD
                     SDGKSYLGSHILDVSMAPSGALELDWDNGKQVGVGQVIGNVLAVACLINCRTVILTMA
                     INRDGSLSGKWSRRTDRGSQGTETWVRT"
     gene            complement(1412..1690)
                     /locus_tag="VFB89_03505"
     CDS             complement(1412..1690)
                     /locus_tag="VFB89_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26396.1"
                     /translation="MGRSITPTPTVDDRLALSWWTYGALILYTTSLLVLCGFVTVQKY
                     SLADGLPTASITANPPHLPIAIYRSGDRVVAGSATPPSCQQASDLSAE"
     gene            2026..>2999
                     /locus_tag="VFB89_03510"
     CDS             2026..>2999
                     /locus_tag="VFB89_03510"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HZM26397.1"
                     /translation="MPKASLPFRLALLVAGMVLPLTVFAAVLVYYNYETSRTTAYDRI
                     LQIARGLTTNIDSELRATIASLQVLALSEALARDDIAAFRAQAEHFVAGHFPQSNVVV
                     TDSSGQQLMNTAVPAGTPLPKYVRMDVLQRVFATGDPQISDLLFGPVLHRNIIVVDVP
                     VWRDGKVVYLLAASLPLSAFSAIILRQRAAPDWTIAVFDKKGTVIARSRDAERFVGRQ
                     ASSTLYPAIMSEFYGVLDTTTFDGTVVLTGFARSPFSGWSVAVGIPKSNLTNQLWRSV
                     AILAGFGILCLAAGIFIAVRLATQLARSQSDRELLINELNHRVKNTLAT"
BASE COUNT          545 a          967 c          930 g          557 t
ORIGIN      
        1 cgaaacgcga tagcgtctgt gttcatcgga gcctgcgcgc cccggcaagg cgtcgcgtgg
       61 cgaaagctag ttcaaacctg caactccacg atttccggtc ttaggtgtcg acccgaaacc
      121 ctgacgcggg cgatggtgac cggcagcttg aaacggcgcg gaccggcgct gcccggattg
      181 agcaacaaaa cgccgttgtg cgtttcgatc gagggccgat gggaatgccc gaacaccacc
      241 gctgcaatgc ccgaagcaac ggggtcggca tcgagctgcg aaatgtcgtg cagaacaaag
      301 aagcgatgct cgccgacctc gacaagctcg cgcatgggca gcgccgctgc ccagtcgcca
      361 tgatcgatgt tcccacgaac cacatgagtg ggtgcgatgt cttcaagccg atcgatcagc
      421 tccggcttac cgacgtcgcc ggcgtggatg atcaggtccg acccgcggag cgcgtcgacg
      481 gcttcgggcc gcagcagccc gtgggtatcc gaaatgacgc cgataacgaa cgcacgcgcg
      541 ggcatctcat gcaccgattg gcatcggatg ggggcattcg atgaatacgt agcacgggga
      601 aacacctggg tgcggctgga acggcgggtg ctatgtcctc acccacgtct cggtgccttg
      661 cgacccccgg tcggtgcggc gtgaccattt cccggacagc gaaccatccc gattgatcgc
      721 catggtcaag atcaccgtgc ggcaattgat cagacacgcc acggccagaa cgttgccgat
      781 gacctggccg actccgacct gcttgccgtt gtcccagtcg agctcgaggg cgcccgatgg
      841 tgccatggag acatcgagga tgtgcgagcc gagatatgat ttgccgtccg agtcggtgcc
      901 ggtcaccacg taggacccca cgagaccagc cttggttgct tcaatcgacc ccaggtcggc
      961 attgcgtgtg acttggaagg gggattcgac cgcggcggcg cgcgtgccga atgcggtgga
     1021 catgccccat ccgctcaggg ctcctgccgc cagcaaaacc gcaccgccac actggcgctt
     1081 ttgccgggtc cgagcctccc gccggatttc atgcctggga ttttcatggt ctctatccct
     1141 cttcgcctgc ttctcagcac ctggtgctcg agtggaccgg ccgcgcctgc ccgcttgcgc
     1201 gggcggttca tgccatcccc accggcttgg gaaggagagg aagctcacag atgcaactaa
     1261 ttatctgcgg atttttttgg aggcccggga gacgtcggcg ccgtctcggc cggcactgaa
     1321 cctgcgggaa gcggctggcg gtggcgcgcc gtcagcgtgt cagactgaaa aaaaccgacc
     1381 gccgagcgcg cgagaatgcg gtccggtccc gttattcggc acttaaatcc gaggcctgct
     1441 ggcaggaggg cggcgtcgcg gaccctgcca cgacccgatc accgctccgg tagatcgcga
     1501 tcggcaaatg cggcgggttc gcggttatcg atgcggtcgg gaggccatcc gccagcgagt
     1561 atttctggac ggtcacaaac ccgcacagca ccaacagcga tgtggtgtaa aggatgagcg
     1621 cgccgtacgt ccaccaactg agcgcaagac ggtcatcgac cgtcggcgta ggtgtgatcg
     1681 atctacccat cgtcgggctc caccgcttcg accccccaca aaacccatac ctcatcacgt
     1741 aatttcatag cgtgaggccc ttcacagtcg ttcgactgcg gcaggctcga tgcgagcgcg
     1801 gtccggcctg ccaccgatag gtcccatggt tcagggcccg agtgggtggc cgggcgcctt
     1861 cgtcgcgacc caccctgcgc ggcgagcagc gtagagcccg cgaacaatcc agtcccacga
     1921 gcgttagccc cggccaacga tcagggctgc cggcctcgca ccaggcatgg cgggcggggc
     1981 cggtcgaatt gttacgctgc ttgccgctcc gacggtgacc gcttgttgcc caaagcatct
     2041 ctcccgttcc gtcttgccct cctggtggca gggatggttc tccctctgac cgtctttgcg
     2101 gcggtgctcg tctattacaa ttatgaaacc agccggacga cggcgtatga ccgcatcctg
     2161 cagattgccc gcggcttgac gaccaacatc gatagtgagc tgcgagcgac gatcgcatcc
     2221 ctccaggtgc tcgcgctatc ggaagccttg gcgcgggacg atattgccgc cttcagagcc
     2281 caggccgaac atttcgtggc cgggcatttt ccgcagtcga acgtcgtcgt cacggattcc
     2341 agcggacagc agctcatgaa tacggccgtg cccgccggga cgccgctgcc gaaatacgtc
     2401 cggatggatg tcctgcagcg ggtgtttgca acgggcgatc cgcagatctc cgacctgctg
     2461 ttcgggccgg tcctgcatcg caacatcatc gtggtagacg tcccggtctg gcgtgacggc
     2521 aaagtggtct atcttctggc ggcctcgctg cccctgagcg cattcagcgc catcatcctt
     2581 cgacagcggg ccgcgccgga ctggaccatc gcggtgttcg acaagaaagg aacggtgatc
     2641 gctcgttccc gggatgccga gcgcttcgtc ggacggcaag cgtcgtcgac tctttatcct
     2701 gccatcatga gcgagttcta cggtgtgctc gacaccacca cgttcgacgg caccgtggtc
     2761 ctgaccggat tcgcccgctc gccgttctcg ggatggagcg tcgcggtcgg aatcccaaaa
     2821 tcgaatctga ccaatcagct ctggcgctcg gtcgccatcc tggccggctt cggaattcta
     2881 tgcctggccg cgggcatatt catcgcggtg cggctggcga cgcagctcgc gcgcagccaa
     2941 tccgatcgcg aactgctgat caatgagttg aatcatcgcg tcaagaatac tctagcgac
//
LOCUS       DASRJQ010000155         5760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1584981, whole genome shotgun sequence.
ACCESSION   DASRJQ010000155 DASRJQ010000000
VERSION     DASRJQ010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5760
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1584981"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            424..879
                     /gene="tnpA"
                     /locus_tag="VFB89_03515"
     CDS             424..879
                     /gene="tnpA"
                     /locus_tag="VFB89_03515"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HZM26398.1"
                     /translation="MPQSLAQIYLHIVFSTKERRRYLQNDAIRTELHSYLGGTCRNLN
                     CPSLCVGGVEDHVHILCRLSRTLAVADLVRELKRESSKWLKTKSPDLAIFEWQAGYGA
                     FSISPGHVEKLREYIAGQEEHHKTESFQDEFRRFLQTYGIEYDERYVWD"
     gene            complement(1048..2007)
                     /locus_tag="VFB89_03520"
     CDS             complement(1048..2007)
                     /locus_tag="VFB89_03520"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HZM26399.1"
                     /translation="MQWLGGNITLRKARLLACAIFRHAPYHPNGQTMWELVPNYRWFS
                     SYLLPYTPLNERRSVEKVMEEHPFRKLTAHEVVELTERAADGIVGALDWDYARSSWAR
                     WHAEPDTWDSMAPEYAGAHLRYSIASSLEDMVSDAARRVLGVCKVLRDVEINHEFGPC
                     VCDLIREMFPDPYETREIDRDWITTDVRGIALHIYHSREFELTPVLADALMDAGCDHE
                     AMILHCRVPRRHVRGCWVIDWILEKDSVRSIPGHVSETTTEGATREEILKCYPSLRSE
                     HIDAAIEYAEWRREVDAAREVAHGKGIDQAAIDRAVEKARYGR"
     gene            complement(2310..3206)
                     /locus_tag="VFB89_03525"
     CDS             complement(2310..3206)
                     /locus_tag="VFB89_03525"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM26400.1"
                     /translation="MTVPSNVFYAFAASQHDPNGWFWKSMGYYPYAYSPWYAPWGYAL
                     GIGLGYGFGYGGYGYGGYGYGGYGYGGGLSFAYHSGSWAIGATIGWPAFGYQAAPVYA
                     PVYAAPYVSSYSPPSTVIVDQPATVAPPVTQILPPPVTESVKPATETNFAVQGMEFYQ
                     AGKYPEAVRALRHAIVDDPRNGNLLALTGQALFAAGSHGEAAGALQQSLAITPEADWA
                     ATSAKMAKLPPAEATESLRKAIDKDPNVPSLRFLAAYQAFGQGEYQQAQTHLDTLLKA
                     APDDAMAKKLHAQAKKLASGKE"
     gene            3265..4440
                     /locus_tag="VFB89_03530"
     CDS             3265..4440
                     /locus_tag="VFB89_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26401.1"
                     /translation="MLDAVVAVEPVVGFEVSGFWPVAAGVPPGFVGLPGVGRFTVAPE
                     VGCVTVADDAPGVGRCAVGLGPTGVSGGLPPPVEPVAGWVTTGFDPPGVGRFTVAPGG
                     GVTTPGITSGDGRFTVGRFTVAPPGGGVTTPGITPDVGREIAPPGVGRFTVAPVDGCV
                     TTPGDAPGVGRETVPPGVGRFTVAPVDGCVTTPGDTPGVGRATVGPGVGREIAAFGAD
                     GMPTGRLGGIATGRGADGMGIGRDIGRGAIAGGRDIPAGRPAAGLPLPCAKTGAASKR
                     APPTVATKYRPNMIAFLPAPGDRKRGDHGVHQRAEENSGLRKCPEWTPEFSLNSDSET
                     ELARAALYQRSHLKSLRNFPTSRSIDESSLVFSSVKSREFANLGSQRHRSRPRCRSS"
     gene            complement(4407..4571)
                     /locus_tag="VFB89_03535"
     CDS             complement(4407..4571)
                     /locus_tag="VFB89_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26402.1"
                     /translation="MYDLPWWVMGLMCLMVLGLLLLLIVLIVVRRGGSRRPLSDIEKD
                     YDERQRGRER"
     gene            complement(4602..4757)
                     /locus_tag="VFB89_03540"
     CDS             complement(4602..4757)
                     /locus_tag="VFB89_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26403.1"
                     /translation="MGMLMVLLLCVLALGLVGLVVVVIAIQRGQRHRGLSDVERDYDD
                     GSRRGER"
     gene            complement(4810..5175)
                     /locus_tag="VFB89_03545"
     CDS             complement(4810..5175)
                     /locus_tag="VFB89_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26404.1"
                     /translation="MNERSRTAILVIAASLVLFAILAALPIWSATRLIKDGPISAITV
                     QGGPLWNADFVEDKGWVRERNWIQAGFWVTVVTVAGLIAYRRRVAKLRLDQSDDYEEK
                     ADGSIPSSRTSPGDPPRTL"
     gene            complement(5190..5339)
                     /locus_tag="VFB89_03550"
     CDS             complement(5190..5339)
                     /locus_tag="VFB89_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26405.1"
                     /translation="MLDMPWWMLGLIVLVPLGLLALGAVLIWAVVTNKEVSRTDYDKD
                     LDRYL"
     gene            complement(5401..5565)
                     /locus_tag="VFB89_03555"
     CDS             complement(5401..5565)
                     /locus_tag="VFB89_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26406.1"
                     /translation="MPDVPDWVKALVCLVLPALFFLIMVFVLLAIACPREPKTDLERD
                     YDDGPRDPPG"
BASE COUNT         1060 a         1826 c         1822 g         1052 t
ORIGIN      
        1 aaggactggg tcgccggcaa ggacccgcag cttgagaaag cgatcgagct ggcgctggaa
       61 gcgctgaaga agaccccgcc gaaggatgac aagcggccgc cgtatccgaa gcgggtgaag
      121 tagcgtctat ctttaccccg aaggggttgc atctcaaagc cgggggtcgc cgcttcggcg
      181 cacgcccggt tggatggcgt gtagggtctt tgcatttacc ccgaaggggt tgcatcacag
      241 agccgggggt cgcgaagcgc acccccggat tggtcgccgt tgttgtgttg tctttacccc
      301 gaaggggttg catctcaaag ccgggggtcg ccgcttcggc gcacccccgg tctccgttcg
      361 caatgcactg tctttgaacc ctgaaagggt tctatcacgc tgacccatca aggagccccg
      421 accatgccgc aatcactcgc ccaaatctac ttgcacatcg tcttctcgac caaagagcgt
      481 cggcgctacc ttcaaaatga tgctatccgt accgaactcc acagctacct gggcgggacg
      541 tgccgcaatc tcaattgccc ctcgctctgt gtcggcggcg tcgaggacca cgttcacatc
      601 ctgtgccggc tgtcgcgaac cctcgctgtc gcggacctgg tccgcgagct gaagcgcgag
      661 tcttcgaaat ggctgaagac caagagcccg gaccttgcca tttttgaatg gcaggccggt
      721 tacggggcct tctcgatcag tccgggacac gtcgagaagc tgcgcgagta catcgccggt
      781 caagaggagc atcacaagac ggagtccttc caggacgagt tccgccgatt cttgcaaacg
      841 tacggcattg aatacgacga gcggtacgtc tgggattgag cgcgtcccgc ggcgcgagcg
      901 tgatagaacc ctttcagggt tcgaaatcca ggtcaccggt ttccgggggt gcgctcgttc
      961 ctcgcgaccc ccgtcttcga gatgcaaccc cttcggggta aagacaggaa aacctgcgag
     1021 atcggcgcaa gcgctcgggt gggaacttca acgtccgtag cgtgcctttt cgacggccct
     1081 gtcgatggcc gcttggtcga tcccctttcc gtgcgccacc tcgcgagccg cgtccacctc
     1141 tcgacgccac tcggcgtatt cgatcgccgc gtcgatgtgc tccgagcgga gggaggggta
     1201 gcacttcaag atctcctcgc gagtggcccc ttcggtggta gtttccgaaa cgtgaccagg
     1261 gattgaacga accgagtcct tctcaagaat ccaatcaatc acccagcatc cgcgaacgtg
     1321 tcgccgcggg acgcggcaat gtaggatcat tgcctcgtgg tcgcaccccg cgtccatgag
     1381 tgcatccgcg agcacgggcg ttaactcaaa ctcgcgactg tgatagatgt gaagcgctat
     1441 gcctcgaacg tcggtagtga tccaatctcg gtcgatttca cgcgtctcgt agggatcggg
     1501 aaacatctcg cgaatcaggt cgcaaacgca gggaccgaat tcatgattga tttcgacgtc
     1561 tcgcaatacc ttgcacacac cgagaactct gcgcgcggca tccgacacca tatcctcgag
     1621 cgaagaggcg atggaatagc gcaaatgcgc ccctgcatat tccggggcca tggagtccca
     1681 ggtatccggt tcggcgtgcc atctcgccca agatgatcgg gcgtaatccc aatccaacgc
     1741 gccgacgatg ccatctgccg cccgttccgt cagttcgacg acttcatgcg ccgtgagctt
     1801 acgaaacgga tgctcctcca tgaccttttc aaccgaccgc ctttcattga gcggcgtgta
     1861 tggaagtagg taagacgaaa accaacgata gttgggcacc agttcccaca tcgtttgccc
     1921 gtttgggtgg tacggcgcgt gtcggaagat ggcacaggcc aggagtcgtg ccttccgcaa
     1981 agtgatattg ccacccagcc attgcaacag cccctcaggc tcgcgacgtc ggttccacct
     2041 gttctttcgt ggcatgggta cgatggcgct gtcttggaaa tgattgtgac ggacactgga
     2101 taggcccgcc gggttcgtgg ctacaggcca ggcattcaaa aaaaatgccg gcgactgccg
     2161 ttcgaggtca gtgggcgggt atgaatcggc tactttccag gcgctttacg gtctgtgcga
     2221 cgacatgaca atcggccgtc cggaaacaaa accggggcag gcgacctccc tggaggcgcc
     2281 cgccccggtt tcgtttcaac ccaccgccct cactccttgc cgctagccag cttcttggcc
     2341 tgtgcgtgga gcttcttggc catcgcgtcg tccggggctg ccttgagcag ggtgtccagg
     2401 tgcgtctgcg cctgttgata ctcgccctga ccgaatgcct ggtacgcggc caggaaccgg
     2461 agggatggta cgttcggatc cttgtcgatc gccttgcgca gggattcagt cgcctcggcc
     2521 ggcggcagct tggccatctt cgccgacgtg gccgcccaat ccgcctccgg tgtaatcgcg
     2581 agcgattgct gcagggcgcc ggcggcttcg ccgtgtgacc cggcggcaaa cagcgcctgg
     2641 ccggtcagcg ccagcaggtt gccgttccgc gggtcatcga caatcgcgtg gcgaagggcc
     2701 cgcaccgcct cggggtactt cccggcctgg tagaactcca tgccctggac agcgaagttc
     2761 gtctcggtcg ccggtttcac ggattccgtc acgggcggcg gcaaaatctg cgtgactggc
     2821 ggggccacgg tcgcgggttg atccacgatc actgtgctcg gcgggctgta cgaggaaaca
     2881 tacggcgctg cataaacggg agcatacacc ggcgcggcct ggtagccgaa cgccggccac
     2941 ccaatcgtgg ccccgatggc ccacgacccg gagtggtagg cgaagctgag gccgcctccg
     3001 tacccgtacc cgccgtaccc gtacccgccg tatccgtatc cgccgtaccc gaacccgtag
     3061 ccgaggccga tgccgagggc atagccccac ggtgcgtacc acggggagta ggcgtagggg
     3121 taatatccca tcgatttcca gaaccaacca ttcggatcgt gctgggacgc cgcgaacgcg
     3181 tagaagacat tgctcggcac ggtcacgcca ttcgccagcg taacggtctg cccatcggca
     3241 gagacggtgt acgggggaaa ccacttgcta gacgctgtgg tcgctgtcga gcccgtagtc
     3301 ggcttcgagg tcagcggctt ctggcccgtc gccgctggcg tcccgcccgg tttcgtcggg
     3361 cttccgggcg tcgggcgatt cacggtcgcg ccggaggtcg gttgcgtcac ggtcgcggac
     3421 gatgcgccgg gggtcgggcg ctgtgcggtt gggctcgggc cgaccggcgt gagtggcggc
     3481 ttgccgccgc cggtcgagcc ggttgccggt tgggtgacga ccgggttcga tccgcccgga
     3541 gtcgggcgat tcacggtggc gccaggcggt ggcgtgacca cgccaggtat tacatccggt
     3601 gacgggcgat tcacagtcgg gcgattcaca gtcgcgccgc cgggtggtgg cgtgacgacg
     3661 ccaggtatca cgcccgatgt cgggcgagaa attgctccgc ccggagtcgg gcggtttacg
     3721 gtcgcgccgg tggatggttg cgtgacgacg ccaggcgacg cgcccggggt cgggcgagaa
     3781 actgttccgc ccggagtcgg gcgatttacg gttgcgccgg tggatggttg cgtgacgacg
     3841 ccaggcgaca cgcccggggt cggacgggcc accgtcgggc ccggagtcgg tcgggagatc
     3901 gccgcgttcg gcgcggacgg catgccgacc gggcggctcg gcggcatcgc gactgggcgc
     3961 ggggccgacg gcatgggcat cggccgggat attggccgcg gagccatcgc cgggggtcgg
     4021 gacatccccg cgggtcggcc cgccgccggc ctacccttgc cctgtgcgaa aaccggcgct
     4081 gccagcaaga gggcaccgcc gacagttgca acgaagtatc gaccgaacat gattgctttc
     4141 ctcccggctc ccggggatcg gaagcgcggc gatcatggtg ttcatcaacg ggcggaggag
     4201 aactccgggc ttcgaaaatg cccggaatgg acgccagaat tcagcctgaa ttctgatagc
     4261 gagactgaac tcgccagggc tgcattatac cagcgctcgc acctgaaaag cttgcgcaat
     4321 tttcccacgt cgcgatcaat cgatgagtcc agcctggtat tctcttcggt gaaatcccgc
     4381 gaatttgcga acctcggttc gcaacgtcac cgctcccgcc cgcgctgccg ctcgtcgtag
     4441 tccttctcga tgtcactgag cgggcgccgc gaaccaccac gccggaccac gatgagcacg
     4501 atcaagagaa ggagcagtcc gagcaccatc aggcacatca atcccatcac ccaccatggc
     4561 aaatcgtaca tcgccaagcc tcccgttttc tcgtcgccgg ttcaccgctc cccccgccgg
     4621 gagccgtcgt cgtagtctct ctcgacgtcg ctgagcccgc gatggcgctg acctctctga
     4681 atcgcgatga ccacgaccac gagaccgacc aacccaagcg ccagcacgca caacagcagg
     4741 accatgagca ttcccatgag gattgaccct tgtcggcttt gtgatttggc aatcgcatgt
     4801 tcgtggacat cagagcgttc gcggcgggtc gcccggcgag gtgcggctgc tggggatcga
     4861 gccgtccgcc ttctcttcat aatcgtcgga ctggtccagg cgcagtttcg cgacgcgacg
     4921 gcgataagcg atcagcccgg cgacggtcac gacggtcacc cagaatccgg cctgaatcca
     4981 gtttcgctcg cgaacccagc ctttgtcttc cacgaaatcg gcattccaga gtgggccgcc
     5041 ttgaacggta attgccgata tggggccatc cttgatcagg cgcgtcgccg accagatcgg
     5101 cagcgccgcg aggatcgcga acaacacaag cgaagccgca atcacaagga tcgcggtgcg
     5161 agagcgctca ttcatcgcgg tctccggtcc taaaggtacc ggtcgaggtc cttgtcgtaa
     5221 tcggtgcgcg agacctcctt gttggtgacc acggcccaga tcaaaacggc gcccagggcc
     5281 agcagaccga gcggcacgag cacgatgagc cccagcatcc accacggcat gtcgagcatg
     5341 attgcgaacc ctccttccga ttgccattac ggtacccgcg aactccggcg ccgcaagggc
     5401 tcagccgggc gggtcgcgcg gaccgtcgtc gtagtcgcgt tcgagatccg tcttgggctc
     5461 ccggggacaa gcgatcgcga gcaggacgaa caccatgatc aggaagaaga gtgccggtaa
     5521 caccaggcag accagcgcct tgacccagtc cggcacgtcg ggcatatctg cgacttccgt
     5581 aggaggccag gctcgcgacc cgaatcgcgg cggagctatc gcgggccgtc gtcgtagtcc
     5641 cgctcgaaat cgttccgcgg acgccgggac cgcgttcggt cgatcatgat gaggatcgtc
     5701 aaaagaacga ctggcagggc cgtggccaga aggttgagtg ccatccacaa cgacgaaagg
//
LOCUS       DASRJQ010000156         3937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1860809, whole genome shotgun sequence.
ACCESSION   DASRJQ010000156 DASRJQ010000000
VERSION     DASRJQ010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1860809"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VFB89_03560"
     CDS             <1..306
                     /locus_tag="VFB89_03560"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM26407.1"
                     /translation="VPFYSEEGSRYLRAELLYQLGRDEEALQWFANGFQGSPHELVYM
                     APAHLRQAEIYERLGDTKRAVEHYSRFIHLWQNCDPELRPRVAEARARLAGLAGEPR"
     gene            complement(456..944)
                     /locus_tag="VFB89_03565"
     CDS             complement(456..944)
                     /locus_tag="VFB89_03565"
                     /inference="COORDINATES: protein motif:HMM:NF025852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZM26408.1"
                     /translation="MRWLLALAALPLMLEPGVLAAQGEHSSSKAIVVTAGTESWGPAP
                     AVLPAGATLAVLEGDPSKSGPYTMRLRMPDGYRIPPHFHPGDEHVTVLSGTFLVGMGE
                     HFDRTGLKALPAGTFGMIPTGMRHFAEAKGVTVIQLHGIGPWSLTYVNPADTPKQVGR
                     SR"
     gene            1060..1731
                     /locus_tag="VFB89_03570"
     CDS             1060..1731
                     /locus_tag="VFB89_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZM26409.1"
                     /translation="MSRPLKDGVYQQFARIGHAVSSPKRLELLDLLAQGEKTVEQLAE
                     QSATALKNTSAHLRVLRQARLVETRRDGSYVLYRLADDAVVRLLRQIEGLGRARLAEV
                     AQVADLYITNRDALEPVSLTELRKRMRDGDVTVLDVRPEDEYVSGHIPGALSIPIAQL
                     RRRLAELPQGREIIAYCRGPYCVYSVDAVEILRKHGYKARRAGEGMPDWRARGYPIAR
                     LQAEA"
     gene            1741..2490
                     /locus_tag="VFB89_03575"
     CDS             1741..2490
                     /locus_tag="VFB89_03575"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZM26410.1"
                     /translation="MILRQFLHADPVIGVSYLLGCGGRAIGAVVDPISAPAWYLRAAK
                     GLGLRIRFVIDTHVHADHVSTGRALAAAAGGEYVLHAEAAARMPFRGVGDGEALELGN
                     VRLDVLHVPGHTPEHIALLATDRTRGSEPWFVLTGHTLMVGDMGRTELASSVEAGARA
                     LFQSAGRLRALPDHIEVLPGAFAGSVCGRGLSGKPTSTIGFERRFNRAFKLGREGEFV
                     ALMMQDIPPRPPNAEAIRATNLGSYVLATNA"
     gene            2487..3803
                     /locus_tag="VFB89_03580"
     CDS             2487..3803
                     /locus_tag="VFB89_03580"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZM26411.1"
                     /translation="MSHELAGGADIAPLRAALATPPVALGLRANWQQFALLVLVNVFV
                     GALVGLERAVLPLLAEAEFGVASRQAMLAFVASFGVAKAAANLLAGRWSARFGRRRVL
                     VLGWLLGIPAPLLVIWAPSWDWVVAANLLLGLNQGLTWSTTVVMKIDLAGPRRRGLAM
                     GLNESSGYLAVAAAALAAGLLADRFGYRPVPFYLGLAIAASGLALTLLFVRDTTDHVR
                     HESQIHSTNSASAAITAAPTFRQIIPLVSWRDRALSSISQAGLVNNLNDGLAWGLLPV
                     FFAASGLDLPRVSMLAFVYPAVWGTAQLVTGPLSDRWGRKGLIAGGMLLQGVALAAIA
                     AQTGAQAYTWWLASSVLLGLGTAMVYPTLLAAIGDVTAPSWRSSAVGVYRLWRDLGYV
                     VGALVAGVTADALGMGAAIYLVAGLTAASGLITAVRMPETMPSRSR"
BASE COUNT          602 a         1315 c         1320 g          700 t
ORIGIN      
        1 gtcccctttt attcggaaga gggctcccgt tatctgcggg ccgagctgct ctatcagctc
       61 gggcgcgacg aggaggcgct tcagtggttc gctaatggat ttcagggctc gcctcacgag
      121 cttgtgtaca tggcacctgc ccacctccgg caggcggaga tctatgagcg gttgggcgat
      181 accaagcggg ctgtggagca ctacagtcgc ttcatccatc tgtggcagaa ctgcgatccc
      241 gagctacgcc cgcgagtcgc ggaggccaga gcgcggcttg cggggctcgc cggcgagccg
      301 cgctgacaat tctgatcgtt gccaaggcac ccctctcatc gctcgtgaac gtgcgatggg
      361 gcggagggcc tcagctctgg ctggcgcgta ggcctcgagt tcgactctcg cctcgggcac
      421 tccgctctcg tcgcgggagc cgcgcgtcgt tggtgctatc ggcttcgacc tacctgcttc
      481 ggcgtatcgg caggattcac ataggtcagg ctccaaggtc cgattccgtg tagctgtatc
      541 acggttaccc ccttcgcctc cgcgaagtgg cgcatcccgg tcgggatcat tccgaatgtg
      601 cctgccggga gtgccttgag tcccgtccga tcgaaatgct cgcccatccc taccaggaac
      661 gtgcccgaga gcacagttac gtgctcgtcg cccggatgga aatgcggtgg gatccggtac
      721 ccatcgggca tgcggagccg catcgtgtac ggaccagact tcgacgggtc gccctcgagc
      781 accgcgagag tagcgccggc cggaaggacc gccggcgcgg ggccccagga ctcggtacct
      841 gctgtgacga cgattgcctt cgatgatgag tgctcaccct gcgcagcgag cacgccgggc
      901 tccagcatga gggggagcgc ggcgagtgca aggagccaac gcataacgga cctccacggg
      961 tgggcggccc gcgcggcata ccccgcatcg ggccggtcag gttgacggag ctggctggag
     1021 ctactattct aacgaatact agaaaagtca aggatcgcca tgtcccgccc cctgaaggat
     1081 ggtgtctacc agcagttcgc gcgcatcggc cacgccgtct ccagtccgaa gcgcctcgag
     1141 cttctcgatc tgctcgccca aggcgagaag acggtcgagc agctcgccga gcagtcggcg
     1201 actgcactca agaacacgag cgcacatttg cgggtccttc gccaggcgcg cttggtcgag
     1261 acccggcgcg acggatcgta cgtgctctac cggttagcgg atgacgccgt agtccggctg
     1321 ctccgccaaa tcgagggact cggccgtgcc cgcctcgccg aggtggcgca ggttgccgat
     1381 ctctacatca ccaaccgtga tgcgctcgag cctgtctcgt tgacggaact ccgaaagcgg
     1441 atgcgcgatg gcgacgtgac cgtgctcgac gtccgtccgg aagacgagta cgtctccggg
     1501 cacatcccgg gggccctctc gatcccgata gcccagcttc ggcgccggct cgccgagctc
     1561 cctcagggcc gcgagatcat cgcctactgt cgaggcccgt actgcgtgta ctcggtagac
     1621 gcggtggaaa tcctacgaaa gcacggctat aaggcgcggc gcgcaggcga aggaatgccc
     1681 gactggcgcg ctcggggcta cccgatcgcg cgcctccaag ctgaggcgtg agcgacgccc
     1741 atgatactgc gccagttcct ccacgcggac ccggtcatcg gcgtgtcgta tctcctcggc
     1801 tgtggtggcc gggcgatcgg cgccgtggtg gacccgattt cagcgcccgc ctggtacctc
     1861 cgggctgcca aaggcctcgg tctgcgcatc cgcttcgtga tcgacacgca cgtgcacgcc
     1921 gatcatgtgt cgaccggccg agcgctcgcc gctgcagctg gcggggagta cgtcctccat
     1981 gctgaggcag ccgccaggat gccgtttcga ggagtagggg acggggaagc cctcgaactc
     2041 gggaatgtac ggctcgacgt tctccatgtc cctgggcaca cccccgaaca cattgcgctg
     2101 cttgcgaccg accgcacccg cgggtcagag ccctggttcg tactcactgg gcacaccctg
     2161 atggtcggcg acatgggccg gaccgagctt gcctcgagcg tggaggccgg cgctcgcgcg
     2221 ctgttccaga gcgccgggcg gctacgcgcg ttgcccgacc acatcgaggt gcttcccggt
     2281 gccttcgctg gatcagtctg cggtcgcgga ctcagtggaa aaccgacgtc caccatcggg
     2341 ttcgagcggc gcttcaaccg cgcctttaaa ctcgggcggg agggcgaatt cgtcgcgctg
     2401 atgatgcagg acatcccgcc gcgccccccg aacgccgagg cgatccgggc tacgaatctc
     2461 ggcagctacg tcctggcgac caacgcatga gtcacgagct ggccggaggg gcggacatcg
     2521 ctccacttcg cgcggcgctg gccacgccac cggtggcgct cggtctgcgc gccaactggc
     2581 agcagtttgc cctgctggtc ctcgtcaacg tgttcgtggg cgcgctggtc ggactggaac
     2641 gcgccgtact tccgctgctg gccgaggctg agtttggggt cgcctcgcgc caggcgatgc
     2701 tcgccttcgt cgcgtccttc ggggtggcca aggcagcggc gaatctgctg gctggtcgtt
     2761 ggagcgctcg tttcggccgg cggcgggtgc tcgtgctggg atggttgctg ggcataccgg
     2821 cgccgctcct cgtgatctgg gcgccatcgt gggactgggt cgtcgccgcc aatctgctgc
     2881 tcggactcaa ccagggactg acctggtcga cgacagtcgt gatgaagatc gacctcgccg
     2941 gcccgcggcg gcgtggcctc gcgatggggc tgaacgagtc ctcaggctac ctggcggtgg
     3001 ccgccgctgc gctcgccgcc ggcctcctgg ccgaccgatt tggctaccgg cccgtaccgt
     3061 tctatctcgg cctcgcgatc gcggcgtcgg ggctcgccct cacgctgcta ttcgtgcggg
     3121 atacaaccga tcacgtccgc cacgaaagcc agatacactc aaccaactcg gcaagcgcgg
     3181 cgatcactgc tgcgccaacc ttccggcaga tcattccgct ggtcagctgg cgcgaccggg
     3241 cattgtcgag catcagccag gccggcctcg tcaacaacct gaacgatggg ctcgcgtggg
     3301 ggctccttcc ggtgtttttc gccgcaagcg gcctcgatct gccgcgggtc agcatgctgg
     3361 ctttcgtcta ccccgcggtt tggggaacgg cgcagctcgt tacggggcct ctgagcgacc
     3421 ggtggggccg caaggggctg atcgccggag gcatgttgtt gcaaggcgtc gcgctcgcgg
     3481 cgatcgccgc gcaaaccggc gcccaagcgt atacctggtg gctcgcgagc agcgtcctgc
     3541 ttggtcttgg aacggcgatg gtctacccga cgctgctggc ggccatcggt gacgtgaccg
     3601 cgccctcgtg gcggagcagc gcggtaggcg tttaccgact gtggcgcgat ctcgggtacg
     3661 tcgtaggcgc gctcgtggcg ggggtcacgg ccgacgccct cggcatgggg gcggccattt
     3721 acctggttgc agggctcacc gcggcatccg gcctgatcac ggccgttcgc atgcctgaga
     3781 ccatgccaag ccgtagccgg tagccactgc cgcacacctc ttcgagcctt gacctgccac
     3841 tgcgacgcta ctgatcgtca ctttgggccg gcccgagctc ggaaggagct cgaggcgtac
     3901 cgcagacgtg gaccgacagg cacggccggg cagctcc
//
LOCUS       DASRJQ010000157         5140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_551461, whole genome shotgun sequence.
ACCESSION   DASRJQ010000157 DASRJQ010000000
VERSION     DASRJQ010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551461"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VFB89_03585"
     CDS             <1..235
                     /locus_tag="VFB89_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26412.1"
                     /translation="PLSFALKPSAHELSHLKHHWASQLPSFVVLDGFTERTFALVLPD
                     LTQVLESRYILADSAMGSRYPVLVYSLRENGGR"
     gene            complement(259..1218)
                     /locus_tag="VFB89_03590"
     CDS             complement(259..1218)
                     /locus_tag="VFB89_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26413.1"
                     /translation="MSSRIYDLSIAVPVVERPIPLDQLYHEFSKPLRDAGYSFEFLFV
                     SHPWDRARTNVLAALSVAGEPVRLIEVVQGGETTLLKVAAANARSRILVTLPAYLQIE
                     ASVLPELVARVRDGADLAVAWRSQRRDSIINRLQNRILHLLVGSLAGGRLHDVACGVR
                     ALRTDLVSEIPLYGDFARFLPLLALREGQIVEEVQATVHQQAMQGRVYGPGVYLRRLI
                     DVLGLFFLIKFTDKPLRFFGLVGTSIASIGVVLMIVLLIQRLTGSGIADRPLLLLSVL
                     LVTLGMQSIALGLIGEMIVHLSAPQQQRYRLRRTSSPARTDAA"
     gene            complement(1215..2165)
                     /locus_tag="VFB89_03595"
     CDS             complement(1215..2165)
                     /locus_tag="VFB89_03595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZM26414.1"
                     /translation="MSGRPELSVVVPLYNEEDSVPALVDAIRSALAAESSWELVLVDD
                     GSADRTAAVAEQLSASDSRIRLIRLARNYGQTQAMQAGFDAAEGDIVVSMDGDLQNDP
                     ADIPKLVNTLEQGYDLVAGYRERRQDKMITRKIPSWVANRLIRAITGVMIRDNGCSLK
                     AYRREALDRMYLYSDMHRFLPALAAATAGARITEVPVRHHARRFGQSKYGLSRIFKIL
                     ADLLTITMISWFGERPIMMFGLASAGASGLGLLFFALSLHSLDATRQRVADAYIYPAA
                     AFLWLLLACYLVMLGLIGEVAVRRLKGRETLLPIAIERSL"
     gene            2495..3652
                     /locus_tag="VFB89_03600"
     CDS             2495..3652
                     /locus_tag="VFB89_03600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZM26415.1"
                     /translation="MVLPTPAGFPLSIVQVAAPAPFGGLEEVVYTLTTGLANRGHAVG
                     LVALVGGGALPPLVERLEAAGVPVERLACGRHYLRERRELAHFFSASRPSLVHTHGYR
                     SDVQAGAVAQRFNLPVMSTVHGFTGGDAKNRIYERIQRRSLRRFGGVIAVSRPLVDSL
                     VASGVARERVHLIPNAYQPRMQVLSRADARAELKLSLPARAFVIGWVGRLSREKGPDV
                     LVDALGLLTDTDAHSVIAGDGPDRAALERRAAALGITERIHWLGVTPNVSRCFSAFDC
                     FALSSRTEGTPIALFEAIAAGVPVIVSAVGGIPDVVSGGEAILVPPNDPGALARALRD
                     VALDPAGAAARAARATVRLRTERSVGPWLDEYEAVYRQLASGAGGSNGRIA"
     gene            3649..4836
                     /locus_tag="VFB89_03605"
     CDS             3649..4836
                     /locus_tag="VFB89_03605"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZM26416.1"
                     /translation="MSLGATVLIAIPIVLGLYAYIGYPLALRLLTAASKRPASFGEPP
                     EWPTISVSLPAYNEEKSIRASLEALLALDYPPERRHILVISDASSDATDEIVREFSGR
                     GVELLRLPHRSGKTAAENAAAPQLRGDIVVNTDATIRIPPSSLKTLIQVFQDPTVGVA
                     SGRDVSVGRMEVETSRGESRYVGYEMEIRRLETRAGGIVGASGCFYAIRRELVSGLFP
                     EALSRDFAAPLIAREAGFRTVSVHEAICYVPRTSSLQSEYHRKVRTMARGLETLWYKR
                     SLANPLRYGRFAWMLLSHKLCRWLVHLTLPLAVVGLLLLTTTSVWAWLVLAGILVVGI
                     LGTVALRWPSHRRMPYLLAGCGFAVAINLAGLQAWSKALRGERNPIWEPTRRPDGASS
                     GSV"
     gene            complement(4833..>5140)
                     /locus_tag="VFB89_03610"
     CDS             complement(4833..>5140)
                     /locus_tag="VFB89_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26417.1"
                     /translation="EQWKTARRLTYLNLILAVVVLLGLGLFVLDYIQLRHAVTGSSRA
                     AYDAAAIKALLVAVLELGVLVWLVVSGFQNFGGVPGSGRKRRRRVGLVVQTPDQEAS"
BASE COUNT          925 a         1610 c         1629 g          976 t
ORIGIN      
        1 ccccctttcg ttcgcgctta agccttccgc ccacgagctg agtcacctga aacatcactg
       61 ggccagccag ttgccatctt ttgtcgtcct cgacggattc accgagcgaa ccttcgcgtt
      121 ggttctcccg gaccttactc aggtcctcga gtcccggtac atcctggctg actctgcgat
      181 ggggtcccgc taccccgtgc tggtctacag cctgcgggag aatggagggc gctaagatgt
      241 accggcggat ggaggcgttc aggcggcgtc tgtccgagcg ggcgaactgg tccggcgaag
      301 tcggtagcgc tgctgctgcg gagcgctcaa gtgcacgatc atctcgccga ttaacccgag
      361 ggcgatcgac tgcattccga gcgtgacaag caggacgctg agcagcaaga ggggacggtc
      421 ggctatcccg gagccggtga gccgttgaat gagcaggaca atcatcagaa ccacccctat
      481 cgacgcgata gaggttccca ccaatccaaa gaaccgcaac ggcttgtcgg tgaacttgat
      541 caggaaaaaa agcccaagca cgtcaatgag gcgccgaagg tatacgcccg gaccatagac
      601 tcgcccttgc attgcctgct ggtggacggt tgcctgcacc tcttcgacga tttggccctc
      661 acgaagcgcg aggagcggca ggaatcgggc aaaatcgccg tacaggggaa tctcggaaac
      721 caggtctgtc cgcagggccc gaaccccgca cgcaacgtca tgcagccggc ccccggccaa
      781 gctgcccacc aacaggtgta ggatgcgatt ctggagtcgg ttgatgatcg agtcgcgtcg
      841 ttgggaacgc catgcgacgg ccaggtcggc accgtcacgc actcgcgcaa ccagctccgg
      901 caataccgac gcttcaatct gaaggtacgc cggcagggtg accaggatgc gactgcgggc
      961 gttggcggcc gccaccttca gcagcgtggt ctctccgcct tgtacgacct cgatcagtcg
     1021 aaccggctca ccggctacgc tcagcgcggc gagcacgttg gtccgcgccc gatcccaggg
     1081 gtgcgatacg aaaagaaact cgaaggaata gcccgcgtcc cggagtggct tcgagaactc
     1141 gtgataaagc tggtcgagcg gaataggacg ctcgaccacc gggacggcga tgctgagatc
     1201 gtagatacga ctgctcacag cgaccgttct atggcgatgg gcaacagcgt ttcccgaccc
     1261 ttcagcctcc gcaccgcgac ctcgccgatg aggccgagca tgacgaggta gcacgcgagg
     1321 agaagccaaa gaaaggccgc ggcagggtag atgtaggcat ctgccacgcg ctggcgtgta
     1381 gcatccaggc tgtgaagact gagggcgaag aacagcaggc caagtccgct cgcacctgct
     1441 gaggccaggc caaacatcat aatcggccgt tctccgaacc acgaaatcat cgtgatcgtg
     1501 agaaggtccg ccaggatctt gaaaattcgc gagagcccgt acttgctctg cccgaagcgg
     1561 cgcgcgtgat ggcgtaccgg gacttcggta atccgggctc ccgccgtcgc cgctgcaagg
     1621 gccggcagaa accggtgcat atctgaatag agatacattc gatccagcgc ttcacgccga
     1681 taggccttga gggagcagcc gttgtcccgg atcatcacac ccgtgatcgc gcgaatcaac
     1741 cggttggcga cccaggacgg gatcttgcgc gtgatcatct tgtcctgacg ccgctcccgg
     1801 taccctgcga ccagatcgta gccttgctcg agagtgttta ccagcttggg aatgtccgcc
     1861 ggatcgttct gcaggtcgcc atccatgctg accacgatat cgccttcggc cgcatcgaaa
     1921 cccgcttgca ttgcctgggt ctggccgtag ttccgcgcca gccggatcag gcggattcgc
     1981 gagtccgacg cagagagctg ctccgctacg gctgccgttc ggtcggcgct gccgtcgtcc
     2041 accagtacca gttcccacga tgactcggcg gctaacgctg agcggatcgc atccaccaac
     2101 gcggggacgc tgtcctcttc gttgtagaga ggtaccacga ccgacagttc cgggcgtccc
     2161 gacatgcttc ggcccttgat agacatgcgt tctcgcggac ctctccaagt tagctttgtc
     2221 actaacccgc ggccacggcg ccacctatgc cagttccgcg ccgcggattc gaaggggcgt
     2281 gcgccagcca gatctgttag ttgcgagggc tggttgctgc tgcaccggct gttgtagctc
     2341 tgccacacag ttctaagccg cgcctccggg cccggccacg acactgctag acttgagcgt
     2401 tgctcgaggg tttccttctg gccggggtcg atcgtatggc gggttcggac cgagatatcc
     2461 tgggcggcat ccgaaaaccg gcacgcggcc tgtaatggtg ctgccgaccc cagccggctt
     2521 tccgttgtcg atcgttcaag tagccgctcc agccccgttc ggagggctgg aagaggtcgt
     2581 gtacacgctg acgaccggct tggcgaatcg cgggcatgct gtaggtctcg tcgcactggt
     2641 tggcggagga gcactgcccc cgctggtcga gcgactcgaa gccgctggag ttccggtcga
     2701 gcgattggca tgtggccgcc actacctgcg agagcgccgg gagctggcgc actttttttc
     2761 ggcgagccgg ccgtcgctgg ttcatacgca cggttaccga tccgacgtcc aagcgggtgc
     2821 cgtggcgcag cgattcaact tgcccgtgat gtcgacggtg cacggcttta ccggcggcga
     2881 tgccaagaac cgtatctacg agcgcatcca gcggcggtcg ctccgacgct ttggtggagt
     2941 gattgccgtg tctcgaccat tggtcgattc gctggttgcg agtggagtgg ctcgagagcg
     3001 cgtgcatcta atcccgaacg cgtatcaacc ccgaatgcag gtgctctcca gggccgatgc
     3061 gcgcgccgaa ctgaagctct cgctgcccgc tcgagctttc gtgattggct gggtgggccg
     3121 actctcgcgg gagaaaggcc ccgatgtact cgtggatgcg cttggattgc tgactgacac
     3181 ggacgctcac agcgtaattg ccggagatgg gcccgatcga gctgctctcg agcggcgagc
     3241 ggccgccctc ggcatcaccg aacggatcca ctggctcggc gtcaccccga acgtgtcgcg
     3301 ctgcttttcg gccttcgatt gctttgcgct cagttcgcgg accgagggca cgcccatcgc
     3361 gctgttcgaa gcgattgccg caggcgtgcc ggtaattgtc tctgcggtcg gcgggatacc
     3421 cgacgtggtc tcgggaggcg aggcgattct ggtccctccc aatgacccgg gcgcgctggc
     3481 tcgcgccctc cgcgacgtcg cgctcgatcc ggccggtgcg gcagcccgtg cagcccgggc
     3541 cacagtccgg ctgcgcacgg agcgcagtgt cggaccatgg ttggatgagt atgaagcagt
     3601 gtaccggcag cttgcctcag gcgcgggcgg ctcaaatggg cggatagcat gagcctggga
     3661 gcaacggtac tcattgcgat cccgatcgtg ctggggctct acgcctatat cggctatcct
     3721 ctggccctgc gtttgctcac cgcggcgtcg aagcgtccgg cctcattcgg agaaccgccc
     3781 gagtggccca ccatcagtgt ctcgcttcca gcctacaacg aggagaaatc gatccgggcc
     3841 tcgttggagg ccttgctcgc actcgactat ccgcccgagc gacgccacat actcgtaatc
     3901 tcagatgcat cgagcgacgc tacggatgaa atcgtgcgcg agttctccgg ccgtggcgtg
     3961 gagcttttgc gcttgccaca ccgttccggc aagaccgccg ccgaaaacgc cgccgcgccc
     4021 cagttacggg gcgacatcgt cgtgaatacc gatgctacga tccggattcc gccaagttcg
     4081 ctcaagacac tcattcaagt gttccaggat cccaccgttg gagtggcatc tggtcgggat
     4141 gtcagcgttg gccgaatgga agtggaaacc agtcggggag aatcgaggta cgtcggatat
     4201 gagatggaga ttcggcgcct ggagacacgg gccggtggaa tcgtcggtgc gagcggttgc
     4261 ttttatgcta ttcgtcggga gctggtgagc ggactttttc ccgaagccct aagccgcgac
     4321 tttgccgccc cgctcattgc gcgtgaggcg ggcttccgaa ccgtatcggt acatgaagcg
     4381 atctgctacg tgccccggac ttcatcgctg cagtccgagt accaccggaa ggttcggacc
     4441 atggctcggg gtctggaaac gctctggtac aagcggtctc tggcaaaccc gctgcgatac
     4501 gggcgattcg cctggatgct gctcagccac aagctgtgcc ggtggctggt gcacctcact
     4561 ctgccgctcg ccgttgtcgg cttgctgctg ctgacgacga cgtcagtctg ggcgtggctt
     4621 gtgcttgcgg ggatcctggt ggtcggcatc ctgggtaccg tcgcgcttcg atggccttcc
     4681 caccggcgga tgccctacct gctggcaggg tgcggcttcg cggttgcgat caacctcgcc
     4741 ggcttgcagg catggagcaa agcgcttcga ggagaacgca accctatctg ggagcctact
     4801 cgccggccgg atggagccag ttccgggagc gtctagctgg cctcctgatc cggcgtctgc
     4861 accactaatc cgacccgccg gcgccgcttt cttccactgc ctgggacgcc tccgaaattc
     4921 tggaacccgc tgacgaccag ccagacgagc acgcccagtt ccaggacagc taccaggagc
     4981 gccttgatcg ctgccgcatc gtaggcagct cggctgctgc cggtgactgc atggcgcaac
     5041 tggatgtaat ccagaacgaa gaggccgagg cccaacagga cgacgacggc caggatgagg
     5101 ttgagatagg tgagtcggcg tgcggtcttc cattgctcga
//
LOCUS       DASRJQ010000158         2097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3834811, whole genome shotgun sequence.
ACCESSION   DASRJQ010000158 DASRJQ010000000
VERSION     DASRJQ010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3834811"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VFB89_03615"
     CDS             complement(<1..484)
                     /locus_tag="VFB89_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZM26418.1"
                     /translation="MIRKGIILAGGSGTRLYPLTRVMNKQLLPVYDKPMVYYPLSTLM
                     LSGIRDILVISTPRDLPHFERLLGSGDDWGLSFQYAAQSTPDGIARAFVVGREFIGPG
                     PVTLVLGDNILFGQGLSGILEAAGRREAGATVFGYRVRDPERYGVAELDAEGQVTGIE
                     E"
     gene            complement(481..1566)
                     /gene="rfbB"
                     /locus_tag="VFB89_03620"
     CDS             complement(481..1566)
                     /gene="rfbB"
                     /locus_tag="VFB89_03620"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HZM26419.1"
                     /translation="MTEPGFRSPNGTVLVTGGAGFIGSAVVRWLLRETDAHVVNLDCL
                     TYAGSLESLDEVSATSRYGFEKVDIRDPAEVARVFAEYQPDAVLHLAAETHVDRGIDR
                     PTAFIDTNIAGTFVLLEAARGYWRSLPADDRARFRFLHVSTDEVYGSLGEDGSFTEAS
                     PYAPRSPYSASKAASDHLALAWYHTFGLPVLVTNCSNNYGPYQFPEKLIPHVMLCALE
                     GRPVPVYGDGGNVRDWLYVDDHVRALLSVLEGGVPGTRYNIGGRNERPNIAVVEQVMS
                     LLDTVRPRPDGESYRGQISFVADRPGHDRRYAVDPVRIERELGWRPHESFETGLAKTV
                     AWYLEHEAWWGRIRGGRYGGERLGTDA"
     gene            complement(1563..>2097)
                     /locus_tag="VFB89_03625"
     CDS             complement(1563..>2097)
                     /locus_tag="VFB89_03625"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZM26420.1"
                     /translation="YDEVAGLGINGKMNEASAIMGLTCLEGLDDFIAINRRNYHAYRK
                     ALAGIAGVQYCEYDERESCNYQYIVLELSANAGLTRDQLVKLLVAEGVVARRYFSPGC
                     HRAEPYRSLAPGTAMGLPVTDLVAGRVVVLPTGTAVDEAAVEEVAGLVRFALDQGPAI
                     ARRLGPVRPSEVYEALG"
BASE COUNT          360 a          733 c          668 g          336 t
ORIGIN      
        1 tctcttcgat cccggttacc tgcccttccg cgtcgagctc cgcgaccccg taccgctccg
       61 gatcccgcac ccgatacccg aacaccgtcg cgcccgcctc gcggcgcccg gccgcttcga
      121 ggatcccgga cagcccctgc ccgaacagga tgttgtcgcc aagcaccagg gtcaccggac
      181 cgggtccgat gaactcacgc ccgactacga aggcccgggc gatgccgtcc ggcgtgcttt
      241 gcgcagcgta ctggaacgag agtccccaat cgtcgccgga cccgagcagg cgttcgaagt
      301 ggggaaggtc tcgcggcgtg gaaatcacca gaatgtcgcg aatgccagac agcatgaggg
      361 tcgagagcgg gtagtagacc atcggcttgt cgtacaccgg gagcagctgc ttgttcatca
      421 cccgggtgag cgggtagagt cgcgtgccgc tcccgccggc gagaatgatc cctttgcgaa
      481 tcatgcgtcc gtccccagtc tctcgccccc ataacgaccg ccgcgaatgc ggccccacca
      541 tgcctcgtgc tccaggtacc acgccaccgt cttcgcgagg cccgtttcga aggactcgtg
      601 tgggcgccag ccgagctcgc gctcgatcct gacggggtcg accgcgtagc ggcggtcgtg
      661 cccgggccga tccgccacga agctgatctg gccccggtag ctctcgccgt cgggccgggg
      721 gcgcaccgta tccaggaggc tcatcacctg ctcgaccacc gcgatattcg gtcgctcatt
      781 acgccctccg atattgtatc gcgttccggg aacgccgccc tccagcacgc tgagcagggc
      841 gcggacatgg tcgtccacgt agagccagtc gcgcacattc ccgccatcac cgtacaccgg
      901 caccggcctc ccctcgagcg cacagagcat gacgtggggt atgagcttct ccggaaactg
      961 gtagggaccg tagttgttgg agcagttcgt caccagcacc ggcagtccga aggtgtggta
     1021 ccaggccagc gcgagatgat cggaagccgc cttgctggcc gaatacggtg accggggagc
     1081 atagggcgac gcttccgtga agctcccgtc ctcaccgagg ctgccgtaga cctcgtcggt
     1141 cgagacgtgc aggaaccgga aacgggcccg gtcatctgcc ggaagcgagc gccagtaccc
     1201 gcgcgccgct tcgagcagga cgaaggttcc ggcaatgttg gtgtcgatga aagcggtggg
     1261 gcggtcgatg ccgcggtcca catgggtttc cgccgcgagg tgcagcaccg catcgggctg
     1321 gtactcggcg aacacgcggg cgacctccgc cgggtcccga atatccacct tttcgaagcc
     1381 gtagcgcgag gtcgccgaca cctcgtccag cgattcgagg ctcccggcgt aggtaaggca
     1441 atccaggttc acgacgtgcg catcggtctc ccgcagcagc cagcgcacca cggccgaccc
     1501 gatgaagccg gcaccgccgg tgaccagcac cgttccgttc ggggagcgga aacccggctc
     1561 tgtcatccga gcgcctcgta gacctcgctg gggcggaccg gacccagacg ccgggcgatc
     1621 gccggtccct ggtcgagcgc aaatcgcacc aggccggcca cctcctccac tgccgcttcg
     1681 tcgacggccg tgccggtcgg cagcacgacg actcgcccgg ccacgagatc ggtcaccggc
     1741 aggcccatgg ccgtcccggg agcaagcgag cgatagggct ccgcccgatg acagcccgga
     1801 gagaaatatc gccgggcaac gactccctcg gccacaagca gcttgacgag ctgatcgcgc
     1861 gtcaggccgg cgttcgcgct cagctcgagg acgatgtact ggtagttaca ggattcccgc
     1921 tcgtcgtatt cgcagtactg cacgccagcg atcccggcga gggccttccg gtacgcatgg
     1981 tagttccgcc gattgatcgc gatgaagtcg tcgagtccct cgaggcacgt caagcccatg
     2041 atggccgacg cctcgttcat cttgccgttg atgccgaggc cggcgacctc gtcgtac
//
LOCUS       DASRJQ010000159         6553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_169606, whole genome shotgun sequence.
ACCESSION   DASRJQ010000159 DASRJQ010000000
VERSION     DASRJQ010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169606"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2186
                     /locus_tag="VFB89_03630"
     CDS             <1..2186
                     /locus_tag="VFB89_03630"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZM26421.1"
                     /translation="TFGIATAPCRATEEAMPEASHTVEAAPDPSVLPAGMPAESQSSQ
                     LLESFPSAAYVCDAEGLIVSFNQPMLKLAGRAPRLHDAGDRFCASLELFQTDGTPIPP
                     EASWVARALRSGKRQTAEEIVLGCLDGRRITAVAHANPLFDETGGVVGAVGVLVDVDT
                     TGRRQVEDALSSIRDELTVQFADLQRLHEMSDRLSSMRELQPILDETLRTAAAIANTD
                     LGLLMLRDEAGDALGVAASLGFSEEGLAAVSSIRNPNGALGMCAQGGRRARVEDVETD
                     AAFAPHRDAARRAGFRALHCTPLINGKGRTIGALSVYHRQPRRPSEREMDLVEVCASQ
                     ATAFIENARLYDALREADRSKNEFLATLAHELRNPLAPIRNAVHVLQLVLHLNGARTE
                     ESKWALDVIERQMQQLSRLVDDLLDLARITSGKLELRKDRIALSDILNAAVEASSPLI
                     DGFGHELVVAEPPEPLFLDGDLARLAQVVSNLLNNAAKYTERGGLIRLIAERRGNEAT
                     ITVRDNGTGIPADMLPLIFDMFNQGGRGADPAQNGLGIGLTLVRRLVEMHGGTVDAHS
                     PGVNQGSELTVRLPLAPDPVPIEAAPLAQSEPAIPRSALRILVVDDNPDSADSLAALL
                     AIPGNQVKVAYDGVEGLALAEEFQPDAALLDLRLPRMDGYEVAQRIREQPWGKGMVLI
                     ALTGWGQEEAKQLSREVGFDQHLVKPVNPAELLELVASLHQSRTP"
     gene            2279..2839
                     /locus_tag="VFB89_03635"
     CDS             2279..2839
                     /locus_tag="VFB89_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26422.1"
                     /translation="MLSEKARFPLAERLRRAGEVTLGEAFAFLSGLYFSGKLAYARRF
                     ARPGPDSWPVQVITTNRGLVPADTLITVHDLRDFGAVDIRTDDPRYRKPMERDAARIC
                     ALPDPEVILLGSVATGKYVDVLLEFFGERLLFPTAFVGRGDMSRGSLLLRAVREEQEL
                     EYEPVATAIRSLSRSAATNWRRGSQE"
     gene            2845..3528
                     /locus_tag="VFB89_03640"
     CDS             2845..3528
                     /locus_tag="VFB89_03640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HZM26423.1"
                     /translation="MPVRKKTTRTRKSRAVSTESRSATGSSTGSALGEYRSKRDFAKT
                     AEPLGSHHAAGRSLAYVIQKHAATSLHFDLRLELDGVMKSWAVPKGPSLDPSVKRLAV
                     QVEDHPIEYNAFEGTIPEGEYGGGTVMIWDRGTYRYDGADPDPMEGLRQGYQKGELKI
                     ALRGKRLKGSWVLVRTKRRQGKRPQWLLIKHRDEVAKPGSEVVDDYETSAATGRTMEE
                     IAAGKRSSR"
     gene            complement(3525..4280)
                     /locus_tag="VFB89_03645"
     CDS             complement(3525..4280)
                     /locus_tag="VFB89_03645"
                     /inference="COORDINATES: protein motif:HMM:NF017102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HZM26424.1"
                     /translation="MMKKGDRGPEVAELQRLLVGRGYPVGIDGEYGTRTYQAVRAFQS
                     QNLDQHGAPLVVDGKVGPLTWWSLHNPKPDLTPPSVVEYGRMPPPEAGGSALGRAALE
                     VAVGELNAGAGEIGGNNAGPFVRKYLGPAGLPEGNSWCASFVSWCYLEASGGDQAVMP
                     FKYCPGARPLLARFRVKGWASAPQDSYQPVPGDPVFWWRVNLTGWQGHVGLVHQLLNG
                     MLYTIEGNRSPRVQGFSYVFSRMDRLLGFGHVP"
     gene            complement(4300..4770)
                     /locus_tag="VFB89_03650"
     CDS             complement(4300..4770)
                     /locus_tag="VFB89_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HZM26425.1"
                     /translation="MALTLTSSAFSAGGAIPGEYTCEGDDISPALEWSGAPAGTRSFA
                     LIVDDPDAPDPKAPKMTWVHWILYDLPATATSLPKGVKKLPTGTREGINDWKRTGYGG
                     PCPPIGRHRYYHKLYALDTVLGDLKNPKKADLEGAMQGHILAQAELMGTYQKAK"
     gene            5062..5796
                     /locus_tag="VFB89_03655"
     CDS             5062..5796
                     /locus_tag="VFB89_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26426.1"
                     /translation="MTRISLATLSLVALTACTSGSKSPSASADSLSRDLSLPASQSNH
                     ALNDRPSTPPKAKPRPKAPAAPSPKSLAAGTLITAQMSDSINSRHDKPGKTVTARVSS
                     DVKDQSGRVVIPAGSPIKLTVTALAPAENKSSKDGKLEFEATSVTVNGRMYALDADVG
                     PVEHELKGRGVTGGDVAKVGAGTAIGAIGGRIIGGNTKGAVIGGVVGAGAGTAYAVHT
                     ADRDVVVHPGTTVRITLRGTLVASAQ"
     gene            5953..6243
                     /locus_tag="VFB89_03660"
     CDS             5953..6243
                     /locus_tag="VFB89_03660"
                     /inference="COORDINATES: protein motif:HMM:NF022815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HZM26427.1"
                     /translation="MGRTKQRRWSRRVTERSNALDLEPGIFRGTPRSIALSLKRSAER
                     SRRRKTDPFRSAMSMLNLYINRAGRKLSAERKRSLDRAKVELRHVFHRKPAS"
     gene            6240..>6553
                     /locus_tag="VFB89_03665"
     CDS             6240..>6553
                     /locus_tag="VFB89_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26428.1"
                     /translation="MTRPPWDGVSHITTTARVVGLVSALLLTRLSKGAMELSEKLPEE
                     AEPHDHLVHLYGRDHHALVTNVGRFFRVGLRRGDSLLAIATLEHCDGFARELHREPEY
                     QPA"
BASE COUNT         1231 a         2100 c         2082 g         1140 t
ORIGIN      
        1 cgacgttcgg catcgcaact gctccttgcc gagccacgga ggaagccatg cccgaggcct
       61 cccacaccgt cgaggccgct cccgacccaa gcgtcctgcc tgccggcatg ccggctgaat
      121 cacagtcgtc ccaacttctc gagagtttcc cttctgcagc atatgtctgc gacgcagagg
      181 gactgatcgt ttccttcaac cagccgatgc tcaagctggc tggccgcgca ccccgcttgc
      241 acgatgccgg cgatcgcttc tgcgcctcgc tcgagctgtt ccagactgac ggcacaccca
      301 ttcctccgga ggcttcctgg gttgctcgtg ccctgcgatc cggaaagcgg cagactgccg
      361 aagaaatcgt gctgggttgc ctggatggac ggcgcattac cgccgtggcc cacgccaacc
      421 cgctcttcga cgagacggga ggtgtagtgg gagccgtagg cgtcctggtc gatgtggaca
      481 ccacgggccg gcgacaagtc gaagacgcac tcagcagcat ccgggatgag ttgacggttc
      541 agtttgccga tctgcagcgc ctgcacgaaa tgagcgaccg gctctcatcg atgcgcgagc
      601 tgcagccgat cctggatgag acgttgcgga ctgccgccgc gatcgccaac accgatttgg
      661 gattgctgat gcttcgcgac gaagccggag acgccctcgg cgtggcagcc agtctcggct
      721 tcagcgaaga aggcctcgcg gccgtaagca gcatccggaa tccgaatggc gctctgggaa
      781 tgtgtgcgca gggtggcagg cgcgcgcggg tcgaagatgt cgagactgac gccgccttcg
      841 cgcctcaccg cgacgcagcg cgccgggccg gcttccgcgc gctccactgt actcccctga
      901 tcaacgggaa gggcaggacc atcggggctc tgtcggtgta ccaccggcag ccccgccgcc
      961 cttccgagcg ggaaatggac ctggtcgaag tatgtgccag tcaggcgacc gcgttcatcg
     1021 agaacgcgcg gctatacgac gccctccgag aggcggatcg gagcaagaac gagttccttg
     1081 cgacgctggc gcacgagctt cgcaatccgc tggcgccgat ccgaaacgca gtccacgttc
     1141 ttcagctcgt gctgcacctg aacggggcgc gcaccgagga gtccaaatgg gcgctcgacg
     1201 tcatcgagcg ccagatgcag cagctgtccc ggctggtgga cgatctgctg gatctcgctc
     1261 gcattaccag cggcaagctg gagcttcgga aagatcggat cgctctgagc gacatcctga
     1321 acgccgctgt agaggcgagc agtcccctga tcgacggatt cggccacgag ctcgtcgttg
     1381 ccgagccacc agagccgctg ttcctcgatg gcgacctcgc ccgcctcgcg caggtcgttt
     1441 ccaacctgtt gaacaacgcc gccaagtata ccgagcgagg tgggctcatt cgcctgatcg
     1501 ccgaacggcg cgggaatgag gcaacgatta ccgtgcggga caacggcacc ggcatcccgg
     1561 cggacatgct gcccctcatc tttgacatgt tcaaccaggg ggggcgcggc gcggaccccg
     1621 cccagaacgg actgggcatt ggattgacgc tggtgcggcg cctggtggaa atgcacggcg
     1681 gcaccgtaga cgcgcatagc ccgggagtaa accagggaag cgagctgacg gtgcggttgc
     1741 cgctggcccc ggacccggtg ccgatcgaag ccgcgcctct ggcccaatcg gagcccgcca
     1801 tcccccgctc ggcgctacgc atcctcgtgg tagacgacaa cccggattcc gccgacagcc
     1861 tcgccgcact tcttgcgatc ccgggaaatc aggtcaaagt cgcatacgac ggcgtggagg
     1921 gcctcgcact ggcggaagag ttccagcccg atgccgccct gctggatctt cgcttgccca
     1981 gaatggatgg ctacgaggtg gcgcagcgaa tccgggagca gccctggggc aaagggatgg
     2041 tgctcatcgc gctcacgggc tggggacagg aagaggcgaa gcagttatcg agagaggtag
     2101 gattcgatca gcacctggtc aaaccggtga accccgctga actgctcgag ctggtcgcct
     2161 cgctccatca gagccggact ccgtagggta agtcgagcag gtcgccattc ggaactcgcc
     2221 tccgtcccgc atctttctga tctcaccggc gcactgcggc ggcaaacgtg ccgagctgtt
     2281 gctcagtgag aaggcccggt tcccgcttgc cgagcggctg cggcgcgcgg gggaggtgac
     2341 gttgggggag gcgttcgcct tcctgagcgg tctctacttc tccggcaagt tggcgtacgc
     2401 gcgccggttc gcccgaccgg ggccggacag ctggccggtg caggtcatta ccaccaatcg
     2461 cggcctggtt ccggccgaca ctctcatcac cgtgcatgac ctgcgcgatt tcggcgcggt
     2521 ggacatccgg accgatgatc cacgctatcg caaacccatg gaacgcgacg cggcccgcat
     2581 ctgcgctttg cccgatccag aggtgatcct gctggggagc gtggcaaccg gcaagtatgt
     2641 ggacgtgctg ctcgagttct tcggcgagag gctcctcttt cctaccgcat tcgtcggcag
     2701 aggggatatg agccgagggt cactcctgct tcgggcggtg cgggaagagc aagagctgga
     2761 gtacgagcca gtcgcaaccg ccattcgaag tttgagccgc tccgctgcaa ccaattggcg
     2821 tcgcggcagc caggagtaga ggcaatgcct gtcagaaaga agaccacccg gacaaggaag
     2881 tcgcgtgctg tcagtaccga gagtcgatcc gcaaccggga gcagcacggg gtcggcgctg
     2941 ggcgaatacc gtagcaagcg ggattttgcc aaaacggctg agccgctagg ctctcatcac
     3001 gccgcgggtc ggagtctggc gtacgtgatc cagaaacacg ccgccaccag tcttcatttc
     3061 gatctacggc tcgagctcga tggcgtcatg aaaagctggg ccgtacccaa gggtccgagc
     3121 ctggatccga gcgtcaagcg tctggcggtc caggtagagg atcatcccat cgaatacaac
     3181 gccttcgagg gcaccatccc cgagggggag tatggcgggg gaacggtcat gatttgggac
     3241 cgcggaacgt accgctatga cggtgccgat cccgatccca tggagggttt gcggcaagga
     3301 tatcagaagg gtgagctcaa gatcgcgctt cggggtaagc gcctgaaagg gtcatgggtc
     3361 ctggtgcgca ccaaacgacg ccaggggaag cgaccgcagt ggcttctgat caagcatcga
     3421 gatgaggttg cgaagccagg ctcggaagtg gtggatgact acgaaacatc cgccgcaacc
     3481 gggcgcacca tggaggaaat cgcggcgggc aaacgcagca gccgctaagg cacgtgtcca
     3541 aaaccgagca ggcggtccat ccggctgaat acataggaga agccctgcac tcgcgggctt
     3601 cggttcccct cgatggtgta cagcattcca ttgaggagtt gatgaaccag tcccacgtgg
     3661 ccttgccagc cggtcaggtt gactcgccac cagaagaccg gatcgcccgg cacgggctga
     3721 tagctgtcct ggggcgcact ggcccaccct ttgacgcgaa agcgggccag taaggggcgg
     3781 gcacccgggc agtacttgaa gggcatcacg gcctgatccc caccggaggc ttcgaggtag
     3841 caccagctca cgaacgaggc acaccaggag ttgccttcgg ggaggccggc gggcccgagg
     3901 tactttcgga cgaatggacc cgcgttgtta cctcctatct cacccgctcc tgcattgagc
     3961 tcgccgacgg ccacctcgag cgctgcccgg cccagcgcac tcccaccggc ttcgggagga
     4021 ggcatgcggc cgtactcgac cacactgggc ggcgttaggt ccggcttggg attgtgcagg
     4081 ctccaccagg tgagcgggcc caccttgccg tcgaccacca gcggtgcacc gtgctgatcg
     4141 agattctgcg attggaaagc gcggaccgcc tggtaggtgc gagtgccgta ctcgccgtcg
     4201 ataccgaccg gatagcctcg ccccaccagc aagcgttgca gctcggcgac ctcgggccct
     4261 cgatctcctt ttttcatcat ggccacgcca cgtcgggcct tacttcgctt tctggtaggt
     4321 acccatcaac tctgcctgag ccagaatgtg gccctgcatc gcgccttcga gatcggcttt
     4381 tttgggattc ttgaggtcgc ccaacaccgt gtccagagcg tagagcttgt ggtagtagcg
     4441 gtggcggccg atcggcgggc aggggccacc gtacccggtg cgcttccaat cattgattcc
     4501 ttcgcgagta cccgtcggaa gcttcttgac ccctttgggc aggcttgtcg cagttgcagg
     4561 cagatcatac agaatccaat gcacccaggt catcttgggc gctttcggat cgggcgcgtc
     4621 gggatcatcc acgatcagag cgaagctgcg ggtcccggca ggagctccgc tccactccag
     4681 agctggacta atgtcgtctc cttcgcaggt gtactcccct ggtatagcgc caccggctga
     4741 gaacgcggat gacgtgagtg taagggccac gaggatctcc tgtagtagca caattcaatc
     4801 actgaaagag gcccgcagac ggtaacgctc tgctgaaggc gtgaccagtg tccggggtgg
     4861 ccgtaatgca ggattccgct caggctcgcg atccgctact gagcgctcga gcgcgggcgg
     4921 tttcccggcg cgtcccgcaa ccgtaaatcc ttgcactgca ataccctgtc ggttggcacc
     4981 ctcgaggcac cgggcttgct catcgacccc cagtcgcccc tgaccacttc ttgtagcgtg
     5041 accccggccc gaggactcct catgacccga atctcactcg cgactctctc gcttgtcgcg
     5101 cttaccgctt gcaccagcgg gagcaagtcg ccctcggcga gcgcggacag cctctcgcgc
     5161 gacctgagcc tccccgcatc gcagagcaac cacgctctga acgatcggcc cagcacacct
     5221 cccaaagcaa agccccgccc gaaagcgccg gctgcgccct cgccgaagtc gctcgctgcc
     5281 ggcacgctca ttacggcgca gatgtccgac tcgatcaact cacgccacga caagccgggc
     5341 aagacggtga cggcccgggt ttcctccgac gtgaaggatc aatcgggacg cgtggtcatt
     5401 cccgcgggct cgccgatcaa gctgaccgtc accgccctgg ccccggcaga gaataagagc
     5461 tccaaggacg gcaagctcga gttcgaagcg acgtcagtga ccgtgaatgg tcggatgtac
     5521 gccttggacg ccgatgttgg cccggtcgag catgagctca agggtcgcgg cgtaaccggc
     5581 ggcgacgtcg cgaaggtcgg tgcgggcacc gccatcggcg ccatcggcgg ccggatcatc
     5641 ggcggaaata ccaagggcgc ggtaatcggc ggcgtggttg gtgccggcgc cggaaccgca
     5701 tatgcggtcc acaccgcgga tcgcgatgtg gtcgtccatc ctggtacgac cgttcgcatc
     5761 acgctgcgcg gtactctcgt cgcatcagcg caataacatc cctggaacag ccagacaaag
     5821 ggcgcccggt tgggcgccct ttttcttgtt gctctgagaa tcgtgaccgg tgccaccgcg
     5881 attctgctcc gcccaggcca aggtcaaaac gagatgggtc gccaagactc caggtctgga
     5941 acggagactg ccatgggacg aaccaagcag cggcgctggt cgcgccgcgt gactgagcgg
     6001 agcaacgcac tcgacctcga gccaggtatt ttccgcggaa cgccgcggag catcgcactg
     6061 tcgctcaagc gttcagcgga gcgtagccgg cgtcggaaaa cggatccgtt ccgctctgcg
     6121 atgtctatgc tgaaccttta tatcaaccgg gcgggtcgca agcttagcgc cgagcgaaaa
     6181 cgcagtctcg accgcgccaa ggtcgagctc agacacgtct ttcatcgtaa accggcatct
     6241 tgacccgccc cccgtgggat ggtgtgagcc acatcacaac aaccgcccga gtggtcgggc
     6301 tagtatctgc cttgctgctg acacgattgt cgaagggtgc aatggaactg agcgagaagc
     6361 tccccgaaga agccgagccc cacgatcatc tggtgcacct atatgggcgc gaccatcacg
     6421 ccctggtcac caatgtgggt cgtttctttc gagtcggctt gcgtaggggc gatagcctgc
     6481 tggcgattgc gaccctcgag cactgtgacg gttttgcccg cgagctacac cgggagcctg
     6541 aataccagcc agc
//
LOCUS       DASRJQ010000160         3333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_169069, whole genome shotgun sequence.
ACCESSION   DASRJQ010000160 DASRJQ010000000
VERSION     DASRJQ010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169069"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VFB89_03670"
     CDS             <1..509
                     /locus_tag="VFB89_03670"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HZM26429.1"
                     /translation="PDIREVVRGLNSGRFLLALSRLTGIDHLISDPYLTGGGVSSVYR
                     GGMLDIHCDGNWHDAMAVHRRLNAILYLNPGWQDSWGGDLEFWDRKMEKCVTKVAPYN
                     NRFIVFVTNDYTFHGHPQPLNCPEHESRRSLILYYYTSSPRNEDEVARDDPHRALWRS
                     RGQLKAAG"
     gene            558..1442
                     /locus_tag="VFB89_03675"
     CDS             558..1442
                     /locus_tag="VFB89_03675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HZM26430.1"
                     /translation="MTLPQLREKASTLLKRTHRLTQALSRRSVDDHIENYLIHDFTNG
                     DYGSSYGIRKKQRVELVERMRTAVASIPSATRMLYHVILARELLKVPASVEGDVVECG
                     VYKGASSASLSLACALAGRRLWVCDSFAGLPDGESDITRSYPHLKQYGHYRKGMYEGA
                     LEEVRENIRRHGDLARCEFVKGLFSESLGILPPRIAFAFVDVDLTVSMQDSIRHIWPR
                     LVDEGQFYTDDSCDMEVVRVWFDDAWWGRELGQRAPGYIGSGCGLPMGVGICSLGYAQ
                     KVASVRTSYQQAAWSASP"
     gene            1424..2869
                     /locus_tag="VFB89_03680"
     CDS             1424..2869
                     /locus_tag="VFB89_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26431.1"
                     /translation="MVRQPLSVRPAIVAAGATALGFAALTVTALAHYQGSTAVFLLFC
                     LSYALLAALIVPAPRLYAYTFFAALLLLGFWLKTVVHVVWEPGFSDPTGDFHNVPEEW
                     DAALLAAACAAAGAALARAAQLLLARRGEGPPTAAPETPVPEYYIRYRKLAWWLTAFV
                     VLALHLLNLRFAFFQVGVNLKLLLPWHLHTLAGWLVNIGLALWISSLAWWEYRWEPAR
                     LSAVLWAPIVEGLLAAASGLSRLAYLLHAGPYWLALLERWAEFRCHLVRRSLIRLAGA
                     FAACFAAITVAVFVLRVLIHYEYAQSVPAGDSVARQTRITIVRQLPQLLVNRWCGLEG
                     VLTVGAVPGRGLELLRAAVTEDPRSGADALYQRYAKPWFLAEKPDKFTFLANAGAVAI
                     LWFSGSLMVVLAGMALVTATMMLTEQLALRLTGNPYLLAVAGAALANVVAQTTFPYLT
                     AVFLAQLWVAILFLGALQRLSPGPPAAERGS"
     gene            2866..>3333
                     /locus_tag="VFB89_03685"
     CDS             2866..>3333
                     /locus_tag="VFB89_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26432.1"
                     /translation="MSDRKGHLSTFLAGATCCTGAGILLIQGAAGYHGSFLIYLAFNA
                     AFLLLPVLVLPRPRLYVYTFFAAFLFLGFWLKAVVQAAWDIGYIEPTGDFAGTSAQWD
                     AALLLATCAALGTAAARIVHLVLARRGGKYLSSPQREPAPAWFVRYRRPVWIAS"
BASE COUNT          472 a         1141 c         1089 g          631 t
ORIGIN      
        1 cgcccgatat ccgcgaggtg gtgcgcgggc tcaactccgg tcgcttcctg ctcgcgctgt
       61 ccaggctcac gggaatcgac cacctgatct ccgaccccta tctcacgggt ggcggcgtga
      121 gctcggtata tcgcggcgga atgctcgaca tacactgcga tggcaactgg cacgacgcca
      181 tggccgttca ccggcggctg aacgccatcc tctatctcaa ccccggctgg caggatagct
      241 ggggcggcga tctcgagttc tgggacagga agatggagaa gtgcgtcacg aaggtggcgc
      301 cctataacaa ccggttcatc gtattcgtga ccaacgacta cacgttccac gggcatccgc
      361 aacctctcaa ctgtcccgag cacgaatcgc ggagatccct catcctctac tactacacct
      421 cgagcccgcg caacgaggac gaggtcgcgc gcgacgaccc gcaccgcgcg ctgtggcgca
      481 gccgtggcca gctgaaggcc gccggctagc gccggatttc cggcgctggt gcgccgccag
      541 acggaagcgg ttcatccatg acattgcccc agctgcggga aaaggcctcc acacttctca
      601 agcgaacgca ccggctgacc caggcattgt cccgccgctc ggtggacgat cacatcgaga
      661 actacctcat ccacgacttc acgaacggcg actacggcag ctcgtacggc atccggaaaa
      721 agcagcgggt cgagttggtc gagcgtatgc ggaccgcggt cgcgagcatc ccatcggcca
      781 ctcgcatgct ctaccacgtc attctggccc gagagctcct gaaagtgccg gcgtcggtcg
      841 agggcgacgt cgtcgaatgc ggcgtctaca agggcgcctc gtccgcgtcc ctgtcgctcg
      901 cctgcgccct ggcgggccgc aggctctggg tatgcgactc gttcgcgggt ctgccggacg
      961 gcgagtccga catcacgcgc agctacccgc acctgaagca gtacggtcac taccgcaagg
     1021 gcatgtacga gggagcgctc gaagaggtgc gcgagaacat ccggcgtcac ggcgacctgg
     1081 cgcgctgcga gttcgtgaag gggctgttct cggagtcgct cgggattctg ccgccacgca
     1141 tcgcctttgc attcgtggac gtggatctga ccgtatcgat gcaggactct atccggcaca
     1201 tctggccgcg cctcgtggac gaggggcagt tctatacgga cgattcatgc gatatggaag
     1261 tcgtgcgcgt gtggttcgac gacgcgtggt ggggccggga gctcgggcag cgggcgcccg
     1321 gctacattgg ctcgggctgc gggctgccta tgggcgtcgg catctgctcg ctcggctacg
     1381 cgcagaaggt ggcgagcgta cgcacgtcct accagcaggc agcgtggtcc gccagccctt
     1441 gagcgtgcgg cccgccatcg tcgccgccgg cgcgacggct ctcggtttcg ccgcgctgac
     1501 ggttacggcg ctagcgcact accaaggcag caccgcggtc ttcctcctgt tctgtctgag
     1561 ctacgccctg ctcgccgcgt tgatcgttcc cgcgccccgc ctctacgcct acacgttctt
     1621 cgcggccctg ctccttctgg gcttctggct caagacggtg gtgcacgtgg tctgggagcc
     1681 cgggttcagc gaccccacgg gcgacttcca caacgtgccg gaggagtggg acgccgcgct
     1741 gctcgccgcc gcgtgcgccg ccgccggcgc ggcgctggcg cgcgccgcgc agttgctgct
     1801 cgctcgacgt ggcgagggtc ccccgaccgc ggcgccggag acgccggtgc cggagtatta
     1861 catccggtac cggaagctgg cgtggtggct cacggcgttc gtcgtcctag cgctgcacct
     1921 tctcaacctg cgcttcgcat tcttccaggt cggcgttaac ctgaagctcc tgctgccttg
     1981 gcatctgcat acgctggcgg ggtggctggt gaatatcggc ctggcgctct ggatctcgtc
     2041 cctcgcctgg tgggaatacc gctgggagcc cgcgaggctg agtgcagtcc tgtgggcgcc
     2101 gatcgtcgag gggctgctcg ccgccgcatc ggggctgagc cgcctcgcct acctgctgca
     2161 cgcgggcccc tactggctcg cgcttctcga gcgctgggcg gagttccggt gccatctcgt
     2221 ccgccggtcc ctgatacggc tcgctggtgc ctttgccgcg tgcttcgctg cgatcaccgt
     2281 ggccgtattt gtgctgcgcg tgctcatcca ctacgaatac gcccagtcgg tcccggcggg
     2341 cgattcggtt gcccggcaaa cccgtatcac gatagtccgg caactgcccc agctcctggt
     2401 gaaccgctgg tgcggcctgg agggcgtgct gacggtgggc gccgttccgg ggcgcggcct
     2461 cgagctgctg cgagccgcgg ttacggagga cccgcggagc ggcgccgacg ccctttacca
     2521 gcgctatgcc aagccctggt ttctcgccga gaagccggat aagttcacct ttctcgccaa
     2581 cgccggcgcc gtcgcaatac tctggttttc gggctcactg atggtcgtgc tcgccgggat
     2641 ggcgctggtg accgcaacca tgatgctgac cgagcaactc gccctgcggc tgaccggaaa
     2701 cccctatctg ctggcggtcg cgggagccgc gctggcgaac gtcgtggctc agacgacctt
     2761 tccctacctc acggcggtgt tcctcgcgca actatgggtc gccattctct ttcttggcgc
     2821 gctgcagcgg ctttcacctg gcccgccggc ggcggagcgc ggctcatgag cgaccgcaag
     2881 ggtcatctca gcactttcct cgccggcgcg acctgttgca ccggcgccgg gatcctgctc
     2941 attcagggcg ccgcggggta ccatggctct ttcctgatct atcttgcgtt caacgcggcc
     3001 tttctcctct tgcccgtgct cgtactgccc cggccacgac tctacgtcta tacgttcttc
     3061 gcggcgttcc tgttcctggg cttctggctg aaggccgtgg ttcaggcggc atgggacatc
     3121 gggtacatag agccgacggg cgatttcgcc ggcacgtcgg cacaatggga tgcggcgctg
     3181 ctgctggcga cctgcgctgc cctcggcacg gccgccgcga gaatcgttca cctcgtcctg
     3241 gcgcggcgtg gcggcaagta cctgtcttca ccgcagcgcg agcccgcccc ggcgtggttc
     3301 gtgcggtacc gccgtccggt gtggatcgcg tcg
//
LOCUS       DASRJQ010000161         2097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1858372, whole genome shotgun sequence.
ACCESSION   DASRJQ010000161 DASRJQ010000000
VERSION     DASRJQ010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1858372"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..340
                     /locus_tag="VFB89_03690"
     CDS             119..340
                     /locus_tag="VFB89_03690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZM26433.1"
                     /translation="MTAPVLPDGELNGLLLTVEEAAHVLRIGRTTMYALVSAGEVQSV
                     CIGRLRRIPFVSLEAYVASLLDTARPDAA"
     gene            350..1579
                     /locus_tag="VFB89_03695"
     CDS             350..1579
                     /locus_tag="VFB89_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HZM26434.1"
                     /translation="MPTRRPNGASSIYFGADGDWHGRVTVGVREDGRPDRRHVRGKSE
                     AEVIRKVRKLERERDAGTVRRAGQRWTVTTWLTHWIDNIAVVPVIADSTHAGYRIDVE
                     YHLIPGVGAHKLDRLAPEHLERLYAKMLRQGKAAGTANHVHRTIRLALNEAVRRGHLS
                     KNPALLAKAPAADEQEIEPYNVDDIKRILKVAGERRNGVRWVIALALGLRQGEALGLQ
                     WTDINLETGELHVRRSRVRPKYRHGCKKACGRKSGFCPERVQTNRPTKNTKSRAGRRV
                     IPLPREVVTLLGVHKERQETERLRAGRLWRGEGWVFAMPDGRPISPNTDFREWKALLK
                     HAGVPSSRLHDARHSSATVLLLLGVPERVVMSVMGWSSTSMTKRYQHVTEAIRRQVAD
                     RLDGLLWESETDGGEGK"
     gene            complement(1655..1743)
                     /locus_tag="VFB89_03700"
     tRNA            complement(1655..1743)
                     /locus_tag="VFB89_03700"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Ser,seq:gga)
     gene            1837..1933
                     /gene="ffs"
                     /locus_tag="VFB89_03705"
     ncRNA           1837..1933
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFB89_03705"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
BASE COUNT          401 a          624 c          715 g          357 t
ORIGIN      
        1 aacggctgcg ctggcaacac gaatacgccg aagccaaggc cgcactcacc gttgccagac
       61 cacccgacaa cacccgttcg gcaactgaac agcacaccgg ttcgatagga ggcaccacat
      121 gactgctcca gtgctcccag atggtgaact gaacggactc ttgctgacgg tcgaggaagc
      181 ggctcacgtc ctccggattg gccgaacgac gatgtacgcg ttggtgtcgg cgggcgaggt
      241 ccagtcggtg tgcattggtc gcctgcggcg tattccgttc gtaagcctgg aggcctacgt
      301 ggcatcgctc cttgacacag ctcggccgga cgcggcgtag gtaggcagcg tgccaacgcg
      361 aaggcccaac ggggcatcct cgatctactt cggggcagac ggtgactggc acggtcgcgt
      421 gacagtgggg gtgcgagagg acggtcgacc cgaccgacgc catgtccgtg ggaagtctga
      481 ggcagaggtc atcaggaagg taaggaaact cgaacgtgaa cgcgatgccg ggactgtgcg
      541 tcgagctggt cagcgatgga ctgttaccac ctggctcact cactggattg acaacatcgc
      601 ggtcgtcccg gtgatcgcgg actcgaccca cgcgggatac cggatcgatg tcgagtacca
      661 cctgatccct ggtgttggtg cacacaagct cgacaggctc gctccggaac acctcgaacg
      721 tctgtacgcg aagatgctgc gacagggcaa agcggcaggg acagcaaacc atgtgcaccg
      781 cacgatccgc ctggccctca acgaggccgt gcgacgcggc cacctgtcaa agaatcccgc
      841 tctactcgcc aaggccccag ccgccgacga gcaagagatc gagccgtaca acgtcgatga
      901 catcaagcga atcctgaaag tcgcagggga gcgccgaaac ggggtccggt gggtgatcgc
      961 attggcgctg ggtctccgtc aaggggaggc ccttggactc caatggactg acatcaactt
     1021 agagactgga gagctgcatg tacgccgtag ccgcgtgcga ccgaagtacc ggcacgggtg
     1081 caagaaggca tgcgggcgta agtctggttt ctgcccggag cgcgtacaga cgaatcggcc
     1141 gaccaagaac accaagtcac gagctggtcg ccgagtcatt ccgctgccga gggaggtcgt
     1201 caccctcctc ggcgtgcaca aggagcgtca agagaccgaa cggttgcggg caggtcggct
     1261 gtggcgaggc gagggctggg tctttgccat gcctgatggt cggccgatct ccccgaacac
     1321 ggacttccgt gagtggaagg cgttgctcaa gcatgctggt gttccctcgt ctaggctcca
     1381 cgacgcccga cattcatccg cgaccgtgct gcttcttttg ggcgttccgg aacgggtcgt
     1441 gatgtctgtg atgggttggt cttcgacgtc gatgacgaag cggtatcagc acgtgactga
     1501 ggcgatccga cgccaggtgg ctgatcggct ggacgggctg ctgtgggagt ccgagacgga
     1561 cggcggcgag ggcaagtgag accgaaagtg agaccagggg gatcagaccg gcctctggct
     1621 gaggtggtcg ggatggtgtt tgtgctggtg agcgtggcgg aggatgcggg attcgaaccc
     1681 gcgagggctg ttaacccaac ccgctttcca agcgagcgcc ataggccact aggcgaatcc
     1741 tccgcgccag aggatacgcg gaacgctcgg cggggcggaa tccgccagtc cgccgaccgc
     1801 gatcgctccg ccaagcggcc gtctcgtaca ctcggcgcag gttccccgtg cggcggtacc
     1861 tcgccgaact cccccagggc cggaaggcag caagggtagg cgagctcttc cgggtgcgcg
     1921 gggagccgtc tttctcccgg tcatcggtca ctcgtggtcc catcgtcggt cacgcgtggt
     1981 gccatcatca gccactcgtg gtcgtacgac gtcggcgatg tggacggagg gcgtgggcct
     2041 gtccgcgacg agcgttaggg ttcggaaggt gactgcaccg ctggcgctct atcgccg
//
LOCUS       DASRJQ010000162         5813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2424546, whole genome shotgun sequence.
ACCESSION   DASRJQ010000162 DASRJQ010000000
VERSION     DASRJQ010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5813
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2424546"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="VFB89_03710"
     CDS             complement(<1..1104)
                     /locus_tag="VFB89_03710"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZM26435.1"
                     /translation="MSRERWDAIQRVLDGALECAPADRVAYVAQACAGDAELQVAVER
                     LLRASEGGTDFLDSPAAEYAAPLARSLDAEVGSVGEGVRVGPFRVLRELGHGGMGTVW
                     LAERDDEFHQQVALKLVRGALAFEDHLVRRFREERQILASLEHPGIARLVDGGVTSEG
                     LPWFAMEYVEGVPLDRYARDRELSLEARLDLFLSVCDAVQYAHRNLIVHRDLKPSNIL
                     VTTDGQVKLLDFGIAKLLRPGDGSAATLTRTALHPVTPEYASPEQLHGEPITVASDVY
                     SLGVTLCELLAERRPHRLAGRQPHEVARVILEQKPDPPSELVSGPLRRRLRGDLDTIV
                     LTAIRTETARRYQTVEQLASDIRRHLQGLPVMAR"
     gene            1298..2377
                     /locus_tag="VFB89_03715"
     CDS             1298..2377
                     /locus_tag="VFB89_03715"
                     /inference="COORDINATES: protein motif:HMM:NF025697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4394 domain-containing protein"
                     /protein_id="HZM26436.1"
                     /translation="MSDSIRRWAEATALTLLTLALPGCGGDGGPIEPPPSEPPPSEPP
                     QQLPAPKGQAIYGVDLANRLVLFGTESPGTLSRKVQITGLPILKRIVGIDFRPSNGKL
                     YGVGNDSRVYIIDPVTGAATAVGTGPFQPAIDFFEVHFGMGFEPATGRIRLMVAESGA
                     NYSINPDDGTAVLETSVSYAKGDVNEGITPRIAGLGYVPLGANALAARRVGLASLGPS
                     PLEGVLLALDADQGTLVESIDPKTGEFVTLGPLLVAFFRCAELKVGLNPDGTMNLFAV
                     VLSEAQNLALKLEESGTGKWVAKKLGFVADDSPIQGIALGKSPAGTAPAMAAAERWVT
                     GAAAPMSYTPTTIDASACTDGPRGK"
     gene            complement(2399..2995)
                     /locus_tag="VFB89_03720"
     CDS             complement(2399..2995)
                     /locus_tag="VFB89_03720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HZM26437.1"
                     /translation="MTASQSSSDESGLEGQQTEITDLLHDLPQRGGEVMERLFPLVYD
                     NLRRIAHRALQAERTDHTLGTTGLVHEAYLKLVDQTRIQYHDRAHFFAIAARAMRQIL
                     VDYARHHRARKRGGGRALNLDAGEVPLDQRAEILLALDEALDRLANLDPRLGQVVECR
                     FFGGLSEEETAEVVGVTTRTVRRDWTKAKGWLREQLSA"
     gene            complement(3006..3542)
                     /locus_tag="VFB89_03725"
     CDS             complement(3006..3542)
                     /locus_tag="VFB89_03725"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZM26438.1"
                     /translation="MKVSLSLGILSVCWISATAAAQSAATPVSDALRSSQERSAKNLI
                     AAAEEFPADKYGFKPTPAQMSVGEVVAHLIEGNDFLCSKVSGTAAPKRTELAKTATKD
                     QLVARLKETFQFCEKALAKVNDSDLGGKMQLFGKREFTRAQAMFITSDDWADHYSQLA
                     IYLRLNGLLPPTAKKPES"
     gene            complement(3589..>5813)
                     /locus_tag="VFB89_03730"
     CDS             complement(3589..>5813)
                     /locus_tag="VFB89_03730"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZM26439.1"
                     /translation="LPYFVMELVQGPPITTFCDSNRLDTRQRLELVIGVCQGVQHAHQ
                     KGVIHRDLKPSNILVRQQDGKPVPTIIDFGIAKATERNPADRAFTTELGAMVGTPAYM
                     SPEQAEASGLDIDTRSDIYSLGIVMYELVTGVLPFEREGRLLAYLGQHALRGGDMPTP
                     SRRVASLASDTASDVAGHRQTTPVGLRRQLRGDLDWITLKAIEHDRARRYETANALGN
                     DIRRYLDDKPVTARPPTTLYTVGKFVRRHRVGVAIGAMAAVALVAVAITMAVLAGRIA
                     RERTRAEREGEKAQAISTFLEDMLKSADPWQGGARQITVVEALKAGVTKLDSSKIRDP
                     LVRASIKRTIGTVYLGLGRTSEADTLLRAALAERIAKAGPETEEAAQSLSDLGALYIE
                     QAKFDSAGLPLERALAIRRKLKGPNDTLVAASLLDLGDLAHERGEYRSEDSLARAALV
                     ILRKAHGKRHVAIANAMRRIVSAQHALENYHEAEAVARRTLQMLKELGMERSVEMSIV
                     LNDLGLSRADQKDYVEAESLLKQVVALDSTNFGPMHPDLAADLENLGYVYFEAGRYDD
                     DIALLKQTLAMRRAMLSDQDPAIGRTIFNMASTIEAKGDTLAAEPMYEEAQKRLLRAN
                     GPDHPNTVVATWVLGRAQYKAGRRREAEANLKAALAVSDPNGKLPPHDYGRVASILAS
                     LLIDERRFPEAEGLALRALAIWDSLADTMRARKSAEQLVTLYDRWGKPDRAAEYRQRR
                     "
BASE COUNT          979 a         1941 c         1841 g         1052 t
ORIGIN      
        1 ccgcgccatg accggcaggc cctggaggtg ccggcgaatg tcggacgcga gctgctctac
       61 ggtctgatag cggcgggcag tttcggtccg gatcgccgtg agcacgatgg tgtcgaggtc
      121 tccgcggagc cggcggcgca gcggtcccga cacaagctcg gacggcggat cgggcttctg
      181 ctcgaggatc accctcgcga cctcatgcgg ctggcgaccg gcgaggcggt gaggtcggcg
      241 ctccgcgagc agctcacaga gcgtcacgcc gagggagtac acgtcgctcg cgactgtgat
      301 aggctctccg tgaagctgct cgggactggc gtattccggt gtgaccggat ggagcgcggt
      361 cctggtaagc gtcgcggctg atccatcgcc tggccgcagc agcttggcga tcccgaagtc
      421 cagcagcttc acctggccat ctgtggtcac caggatgttc gatggcttga gatcacgatg
      481 aacgatgaga ttgcggtgag cgtactgcac agcgtcgcag actgacagga agaggtcgag
      541 ccgggcctcg agcgacagct cacggtcccg cgcgtagcga tcgagcggga ccccctccac
      601 gtactccatc gcgaaccagg ggagtccctc agacgtcacg ccaccgtcga ccagacgtgc
      661 aattccgggg tgctcgagcg acgcgaggat ctggcgctcc tcgcgaaagc gccggaccag
      721 gtgatcctcg aacgcgagcg ccccacggac cagcttgagc gccacctgct ggtggaactc
      781 atcgtcccgt tcggcgagcc acaccgttcc catccccccg tggcccagct cgcgcagcac
      841 ccgaaacggc ccaacgcgga cgccttcacc gacggacccc acttcggcgt cgagactgcg
      901 tgccaggggc gcggcgtact ccgcggccgg cgagtcgagg aagtccgttc ctccctcgga
      961 ggcgcggagc aggcgctcca ccgcgacctg aagctcggca tcgccggcgc acgcctgcgc
     1021 gacgtaggca acgcggtccg caggggcaca ctcgagcgcg ccatccagca cgcgctggat
     1081 cgcgtcccac cgctcccgcg acatcgctat gtccacctgc gggcccagag cggaacacct
     1141 ccctccgctg ccgtcccccg ccgctttttc gggaggtagc tcgctcaaca tgcgggtcgg
     1201 aacgcggtct cgcaatccgg cggatgtccg cttttggcca gcggtttcgc cttaacgggt
     1261 gaagcagaag caccgtgcca tcccgctgga ggtcccgatg tccgacagta tccgccgatg
     1321 ggccgaagcc actgccctca cgctgcttac ccttgccctc cccggctgcg gtggcgacgg
     1381 tgggccgatc gagccgccac cctccgaacc gccgccgtca gagcccccac agcaactgcc
     1441 ggccccaaaa ggccaggcga tttacggcgt ggacctcgcc aaccgactcg tcctgttcgg
     1501 taccgagagt cccggcacac tgagccgcaa agtgcagatc acaggactcc ccatcctcaa
     1561 gcgaatcgtc gggatcgact tccgcccctc gaacggaaag ctctacgggg tcggaaatga
     1621 ctcccgggtc tacatcatcg atcctgtcac cggcgcagcg accgcggtcg gaaccgggcc
     1681 attccagccg gcgatcgact ttttcgaggt gcatttcggg atgggattcg agccggccac
     1741 tgggcggatt cggttgatgg tcgccgagtc gggagccaac tactcaatca acccggacga
     1801 cggaaccgcg gtgctggaga ccagcgtgag ctatgcgaag ggggatgtca acgaagggat
     1861 cacgccgaga attgccgggc tcggctacgt tccgctcggg gctaatgccc tagccgcgcg
     1921 ccgcgtcggc ttggcgtcgc tcggtccgtc gccgcttgaa ggggtgctgc tggcgctgga
     1981 cgccgatcag ggcacgctgg tcgagtcaat cgaccccaag acaggcgagt tcgtcaccct
     2041 cggcccgctc ctggtggcat tcttccggtg cgcggagtta aaggtcggcc tcaatccgga
     2101 cgggaccatg aatctcttcg ctgtcgtcct gagcgaggcc cagaacctcg ccctgaagct
     2161 cgaggagtcg gggaccggta aatgggtggc gaaaaaactt ggattcgtgg ccgacgattc
     2221 gccgatccag ggaatcgcgc tagggaagtc gccagccggg accgcgcccg ccatggcagc
     2281 tgccgagcgg tgggtaaccg gtgccgccgc acccatgtcg tacacgccga ccacgatcga
     2341 cgcgagtgcg tgcacggacg gcccgcgggg gaagtgatcc ccgcgggcat ttagcccgtc
     2401 aggcactcag ctgttcacgg agccacccct tcgccttggt ccagtcgcga cgcaccgtac
     2461 gcgtagtgac gcccacgacc tccgcggttt cctcctcgct caatccgccg aagaaacggc
     2521 actcgaccac ctgccccagg cgggggtcga ggttcgcgag ccggtcgagc gcttcgtcaa
     2581 gcgccagcag tatttcggcc cgctggtcca gcggcacctc gcccgcgtcg agattgagag
     2641 cacgcccgcc gccacgcttc cgagcccggt ggtgccgcgc gtagtccacc agaatttgtc
     2701 gcatagctcg cgcggctatg gcgaagaaat gagcccggtc atggtactga atccgggtct
     2761 ggtcgaccag tttgagatac gcctcatgca ccaggccggt cgtcccgagc gtgtgatcgg
     2821 ttcgctccgc ctggagtgct cggtgagcga tgcgccgaag attgtcgtag accagcggga
     2881 agagccgctc catgacctcg ccacccctct gcgggaggtc gtgcaggaga tcggtgatct
     2941 cggtctgctg gccttccaga ccagattcgt ccgagctgga ctgcgaggcc gtcacggacg
     3001 gcgtcttacg actccggctt cttcgccgtt ggcggcagga ggccgttcag ccggaggtag
     3061 atcgcgagct ggctgtagtg gtccgcccag tcatccgaag taatgaacat cgcctgagcg
     3121 cgggtgaatt cccgcttccc gaacaactgc atcttcccac ccaggtccga gtcattgacc
     3181 ttggccagcg ccttctcgca gaactggaag gtttccttga gccgggcgac gagctggtcc
     3241 ttcgttgccg tctttgccag ctcggtgcgc ttgggtgctg ccgtgccgct gaccttggag
     3301 caaagaaagt cgttgccttc aatgaggtgc gcgaccacct cgcccacact catctgcgcc
     3361 ggggtgggct tgaagccgta cttgtcagca ggaaactcct cggccgcggc gatcagattc
     3421 ttggccgagc gctcctggct ggagcgaagc gcatcggaca cgggggtcgc ggcactctgc
     3481 gctgcggcgg ttgccgagat ccagcagacg gacagaatgc ccagtgaaag tgagactttc
     3541 attgcgatgc tccgttgagt cgagtaaaca agcgaaacca gaaaaaagtt accgtctctg
     3601 ccgatattcc gccgcgcgat ccggcttgcc ccagcgatcg tagagcgtca ccagctgctc
     3661 ggctgacttc ctggcacgca tcgtatcggc caaactgtcc caaatggcga gggctctgag
     3721 tgcgagcccc tcggcctcgg ggaacctccg ctcgtcgatg agcaaggatg ccagaatgga
     3781 agccaccctg ccgtagtcgt gcggtggcag cttcccattg ggatcgctta ccgcgagcgc
     3841 tgccttgaga ttcgcctcgg cctctctgcg tcggcctgcc ttgtactgag cccgacccag
     3901 cacccaggtg gctacgacgg tattgggatg gtcaggccca ttggcgcgca gcagccgctt
     3961 ctgggcctct tcgtacatgg gctccgcggc cagggtatcg ccctttgctt caatcgtcga
     4021 agccatgttg aagatggtgc ggcctatcgc cggatcctgg tcgctcagca tcgctcgccg
     4081 catggcgagg gtctgcttga gcaacgcgat gtcgtcgtca tagcgtccgg cctcgaagta
     4141 cacatatcca agattttcga gatccgccgc cagatccggg tgcatgggcc cgaagttcgt
     4201 ggagtcgagc gccacgacct gcttgagcag gctttcagct tccacgtagt ccttctgatc
     4261 ggcccggctg agcccgagat cgttgagcac aatcgacatc tcgacgctcc gttccatgcc
     4321 gagctctttc agcatctgca atgtccggcg cgcgaccgcc tcggcctcat ggtaattctc
     4381 cagagcgtgt tgtgccgaga caatccggcg catcgcgttc gcaatcgcga catggcgctt
     4441 gccatgcgct tttcgcagga tgaccagtgc cgcccgggcc agcgaatcct cactgcgata
     4501 ctcaccccgt tcgtgcgcca gatccccgag atccaacagg ctggccgcga ccagagtgtc
     4561 gttagggccc ttcaacttcc gccggatcgc cagggcacgc tcgaggggca ggcctgccga
     4621 atcgaatttc gcctgctcga tgtagagagc acccaaatcg ctgaggcttt gggctgcctc
     4681 ctcggtctcc ggtccagcct tcgcgatccg ctccgccagg gccgcccgga gcagggtatc
     4741 tgcctcgctg gtgcggccca atccgagata cacggttccg atcgtgcgct tgatcgatgc
     4801 gcggaccagt ggatcgcgaa tcttgctgct gtccagcttg gtcacaccgg ctttgagggc
     4861 ctcgacgacg gtgatctgcc gcgcgccgcc ttgccatgga tcggcggact tgagcatgtc
     4921 ctcgagaaag gtgctgattg cctgggcctt ctcgccttcc cgttcagccc gggtccgctc
     4981 ccgcgcgatt cgcccggcca gcacggccat cgtaatcgcg acggccacca gtgccaccgc
     5041 tgccattgct ccgatcgcaa caccgacgcg atgccgcctt acgaatttcc caacggtgta
     5101 cagcgtggtc ggcggacggg cagtaaccgg cttgtcgtcc aggtaacgcc ggatgtcgtt
     5161 gcccaatgca ttcgcagtct cgtaacggcg cgcgcgatca tgttcgatag ccttgagcgt
     5221 gatccaatcc aggtcgccgc gcagctgtcg tcgtaacccc accggcgtcg tctgccggtg
     5281 gcctgcaacg tcgctcgcgg tgtcagacgc gagactcgcg actcgccggc tgggcgtggg
     5341 catgtcgccg ccacgcaagg cgtgctggcc aaggtaagcc agcaatctgc cctcccgttc
     5401 gaacggcagc accccggtga ccagctcgta catcactatg cccaggctgt agatgtcgga
     5461 tcgggtatcg atgtcgagac cagaggcttc cgcctgctcc gggctcatgt aggccggcgt
     5521 tcccaccatc gcgcccagct cggtggtgaa cgcgcggtcg gccggattgc gctcggtcgc
     5581 cttggcgatt ccgaagtcga tgatggtagg gaccggcttg ccgtcctgct gccgcaccag
     5641 gatgttcgag ggcttgaggt cccggtgaat gacacctttc tggtgcgcgt gctgaacgcc
     5701 ctggcagacc ccgatcacca gctcgagccg ctggcgagtg tcgagccggt tggaatcgca
     5761 gaatgtggtg atcggcggcc cttgcaccag ctccatgacg aagtagggca gcc
//
LOCUS       DASRJQ010000163         3181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1580422, whole genome shotgun sequence.
ACCESSION   DASRJQ010000163 DASRJQ010000000
VERSION     DASRJQ010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1580422"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VFB89_03735"
     CDS             complement(<1..471)
                     /locus_tag="VFB89_03735"
                     /inference="COORDINATES: protein motif:HMM:NF021361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HZM26440.1"
                     /translation="MTKSPFTHEDLMQAHGSVLRRCWLILFATVALPLGLQAQAPDQD
                     RMALARQVIKASGAEALMVSSIDANLPTQREKNPSVPAEFWDRIAARARADIGGLVDS
                     LAPIYAKRFTKAELEQLLTFYKSPVGRHVLAEQGSIAQESQQMGMRWGTRLGGAV"
     gene            complement(468..815)
                     /locus_tag="VFB89_03740"
     CDS             complement(468..815)
                     /locus_tag="VFB89_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26441.1"
                     /translation="MSRFAWTDQVANISAALHALEEQVAQGDVSAPGLADFKSALDDL
                     RLRAWGLLMATSADDYQGFQQRFRIRRGKEMCRSLDVDLRAGKLSPRHPELPELGAAA
                     RDLVTTVDKTRTA"
     gene            1438..1992
                     /locus_tag="VFB89_03745"
     CDS             1438..1992
                     /locus_tag="VFB89_03745"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HZM26442.1"
                     /translation="MIRFASLFLLSVMLVPGATAQAQAVAHDSALSSVGAAALHDSVA
                     SVSRSQLGTRYRLGAAQPGRRFDCSGLVQYVMSVFHVLLPRTAAAQAQLGVAVPRDAT
                     QLLPGDILTFGRGRRITHVGIYVGDGRYIHASSRRRTVVEVPLPDPDTRQGRLWKGAR
                     RVLNASARADTDSVPRPIVESPAS"
     gene            1989..>3181
                     /locus_tag="VFB89_03750"
     CDS             1989..>3181
                     /locus_tag="VFB89_03750"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HZM26443.1"
                     /translation="MNRPMDYARAAGDVNSFRVPMRRSFRILAFLSAVCGLSAPGASA
                     QTISPDLARSMDEWFARSQRIAPGQWGVAVATSTGRVLWTANPETQLIPASTAKIFTT
                     GFVRGIVGPDARIATRVVGRGHLDSAGVWIGAWGLELNGDPTLERTVRSGPMLRDLAR
                     KLSAVGVRELRGPMDLTSTTGTTDTRIPAVWGDRYVGQLYAPPVGCVTLHENRVGWSL
                     RPAGLIGAPPTIVYAIPAGSEALVKVTARTVPGSGRKLTLTALAGGTWELSGTLGIYS
                     RIAGVSTISQRPDLVLREAWGAALSRAGIRWVRDGKPTRPGLLERADMLAEVTSAPFD
                     SLAMMVNRRSLNIGAEALLRWADGSSNAAERLTRHVQEVVGAGAAVKLVDGSGLSPLD
                     RVSPLT"
BASE COUNT          494 a         1102 c         1006 g          579 t
ORIGIN      
        1 gaccgccccc ccgagccgag tgccccagcg catccccatc tgctggcttt cctgagcgat
       61 cgatccctgc tcggccagga cgtgccgccc caccgggctc ttatagaagg tgagaagctg
      121 ctccagctcc gccttggtga agcgcttcgc atagatcgga gcaaggctgt ccaccagacc
      181 tccgatatcg gcccgcgccc tggccgcgat gcgatcccaa aactccgcag gaaccgatgg
      241 gttcttctca cgctgggtcg ggaggtttgc gtcgatgctc gagaccatga gcgcctcggc
      301 cccgctcgcc ttgatgacct gccgcgccaa cgccattcga tcctggtcgg gtgcctgtgc
      361 ctggagaccc aatggcagcg cgaccgtcgc gaagagaatg agccagcatc tgcgcaagac
      421 tgaaccatgg gcctgcatga ggtcctcgtg ggtaaagggt gacttggtca agcggtgcgc
      481 gtcttgtcca ccgtcgtcac caggtcacgc gcagcagccc ccagctccgg cagctcggga
      541 tgccgggggc tcagcttgcc cgcacgcagg tccacgtcga gcgaccggca catctccttg
      601 ccgcgccgga tccggaatcg ctgctgaaag ccctggtagt cgtcggcgct ggtcgccatc
      661 agcaaacccc acgcccgcaa gcgcaggtcg tccagcgcgc tcttgaagtc ggccaggccg
      721 ggcgccgaga cgtcgccttg agctacctgc tcttccaggg cgtgcagcgc cgcgctgatg
      781 ttggccacct ggtcggtcca ggcgaaccgt gacatggtca ctcctcctgt tccgtgttag
      841 tgagaccccg ctttaactcc tagacctgga gcctggttgg gatgcgaatc gtcgcgcata
      901 tccgtatttg atggtccgaa ctgcgagagt tcagagcccg taccgcgcat atgactgcag
      961 tgtggatgat ccagaaggat ttgccgcgac gattccgact tcctggcaga ggtccgccgc
     1021 cgcatctcta caacgatggt ggaccgggtc cctcggcgcg aggctggttt tgcgtcaggt
     1081 atggtccctt ctgcgacagc acccccgccc agcgaacctc agacgacacc agaggcgacc
     1141 aggcctaacc cccggtttca tatgaacctc tggacttcag accgtttcct tctattatcc
     1201 tggcgtgtcg gacacggcac gcccgttgcc ctagtcggca accggtgcgt gaggcgccgc
     1261 gcctcgacac ggcgtcggtt tgccggcgcc cctggtcgga cccgatagat agccattcaa
     1321 tcgagcacaa ccgtcacgtt tcccgacgcc gttcgcgtcg gcaagccaga cgttgcacca
     1381 gttgcgtgct gcttttgctc tggtcaggcg cccgacgtcc tgcaataggc gagagcgatg
     1441 atccgtttcg cgagtctgtt cctcctgtcg gtaatgctcg tgcccggggc gactgcgcag
     1501 gcacaagccg ttgctcacga ctcggcgctc tccagcgtcg gcgccgccgc gctgcacgat
     1561 tccgtcgcct cggtttcccg ctcccagttg ggcactcgct atcggctggg tgccgcgcag
     1621 cccggtcgcc gtttcgattg cagcgggctc gtgcagtacg tgatgtcggt ctttcacgtg
     1681 ctgctccccc ggacagcagc cgcgcaggcc cagctcggcg tcgccgtccc ccgtgacgcc
     1741 actcaactcc ttcccggcga catcctgacc ttcggccgcg gccggcgcat cacacacgtc
     1801 ggcatctatg taggcgacgg tcggtacatc cacgccagca gtcggcgccg tacagtcgtc
     1861 gaggtgccgc tcccggatcc cgacactcgg cagggtcggc tctggaaggg agctcgacgg
     1921 gtactgaacg ccagtgctcg cgccgatact gacagcgtcc cgcggcccat cgtcgagtcg
     1981 ccagcatcat gaaccgaccc atggattacg ctcgtgcagc aggagacgta aactctttcc
     2041 gggttcctat gcgacgctcg ttccggatcc tcgcgttcct gtctgccgtg tgcggtcttt
     2101 cggcgcctgg tgccagcgca cagacaatct cgcccgacct tgcccgctcg atggacgagt
     2161 ggttcgcccg ctcccaacgc atagcaccgg gacagtgggg cgtggcggtc gcgacgtcga
     2221 ccggccgggt gctctggact gccaaccctg aaacccagct catccccgcc tccacggcca
     2281 agatcttcac caccggattt gtccgtggaa tcgtgggacc cgatgcgcga atcgccacgc
     2341 gcgtggtggg ccgggggcat ctggattccg cgggcgtctg gatcggcgcc tggggcctcg
     2401 agttgaatgg cgatcccacg ctcgagcgca cggttcgttc aggaccgatg ctgcgcgacc
     2461 tcgccaggaa gctgagcgct gtcggggtac gtgagctgcg cggcccgatg gacctgacca
     2521 gtaccaccgg caccaccgat acgcgcatac ccgcggtgtg gggcgaccgt tatgtcggcc
     2581 agctgtacgc gccgccggtg ggttgcgtga cactgcacga gaatcgggtc ggctggagtc
     2641 tcaggcccgc cggtctgatc ggcgctccac ctaccatcgt ctatgcgatt cccgctggtt
     2701 ccgaggcgct ggtgaaggtc accgcccgaa ccgtgcccgg cagcgggcgg aagctcacgc
     2761 tgaccgcgct ggccggcggg acctgggagc tgagcggaac gctcgggatc tattcccgaa
     2821 tcgccggcgt ctcgaccatc tcgcaaaggc ccgacctggt gctgcgcgaa gcctggggtg
     2881 cggccctctc tcgcgccggt atccgctggg tacgcgacgg caaaccgacc cgaccgggac
     2941 ttctcgagcg tgccgacatg ctcgccgaag tcacctccgc gccgttcgac tcactcgcca
     3001 tgatggtgaa ccgccggagc ctcaatatcg gcgccgaggc gctgctccgg tgggccgacg
     3061 gctcgagtaa cgccgccgag cgcttgacgc ggcacgtgca ggaggtcgtg ggcgcgggcg
     3121 ccgcggtgaa gctggtggac gggagtggac tctcgccgct cgaccgggta tcgccgctca
     3181 c
//
LOCUS       DASRJQ010000164         2219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_498712, whole genome shotgun sequence.
ACCESSION   DASRJQ010000164 DASRJQ010000000
VERSION     DASRJQ010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498712"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            498..1319
                     /locus_tag="VFB89_03755"
     CDS             498..1319
                     /locus_tag="VFB89_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZM26444.1"
                     /translation="MSTITTKDGTEIYFKDWGTGPVVTFSHGWPLNADAWDGQMLFLE
                     QKGYRCVALDRRGHGRSSQASANNDMNGYADDLAAVIEALDLRDATLVGHSTGGGEVA
                     RYIGRHGSKRVRKAVLVAAVPPIMLKSPANPEGLPMEVFDQIRAGVAGDRSQYYKDLA
                     IAFYGANRPGAKVSQGTLDQFWLWSMQAGAKNAYESVKAFSETDFTEDLKKFDVPTLV
                     LHGEDDQIVPVKDSAKKSAKLIKGAKEIYYPGAPHGLTATHQERVNADLLAFIEG"
     gene            1457..>2219
                     /locus_tag="VFB89_03760"
     CDS             1457..>2219
                     /locus_tag="VFB89_03760"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZM26445.1"
                     /translation="MSLDNTPHPSSKHCADTVHARYTHGRPEHELVPSRYALKVGAID
                     VMVVSDGVLSLPGAMLGHNVDPAIRAAWLEDMFLPPDVLEWALNAVVVRSGGRTILVD
                     AGIGAEFPDLPRAGQWAQRLEAAGIDPASVTDVVLTHLHMDHCGGLLADGVKERLRPD
                     LRVHVAAAEAAFWEWPDFSRVSMPPGFPDALRRTGKRFLKEFSSQLRTFENEYEVAPG
                     VVVQRTGGHTPGHSVVRLASGGDRLTFAGDAVFQVG"
BASE COUNT          403 a          687 c          741 g          388 t
ORIGIN      
        1 aacggcttcg aacacgaccc cgcggaagca gcccgcgtac gggtccatct tttgcgggag
       61 ctggcggcga ctggcgaggc gctggtggcc actcacctgt cgttcccgtc cgtctgccat
      121 gttgcggtcg acggcgacgt ctttcgatgg gtaccgggcc cctgggagta ctgaccgctt
      181 gttgggttag gtccgaacgg gagtcatcgc gtcatgatcg tctgctcgtg caaatgtgct
      241 gagcgatcag gatatccgct ccgccgtcgc gggtgatcta tgcaaaggtc cgcatatcgg
      301 gtctccgcct tcgggactaa cttctttgcg ggccgcgcat gtcctccttg gtgcgcgtgc
      361 gctcctggcg caccgcgccc gtacgtcagc aatgcgatgg ccccgagggg cgctggctgt
      421 tttgtgccca atgaactgcc ccacgtcacg ccaacgccca gcgcttactc tcacaatcag
      481 acaagtggac gcacgccatg agcacgatca caaccaaaga cggcacagaa atctatttca
      541 aggactgggg aacaggccca gtcgtcacct tctcgcacgg ttggccgctc aatgccgatg
      601 catgggatgg tcagatgctg ttcctcgaac agaagggcta tcgctgcgtc gcactcgacc
      661 gcaggggtca tggccgctca agtcaggcgt cggccaacaa cgacatgaac ggctatgccg
      721 acgacctcgc tgccgtaatc gaggcacttg acctcaggga cgcgacgctg gttggccact
      781 ccacgggtgg cggagaggtc gctcgctaca tcggtcgtca tggcagcaaa agggtgagga
      841 aggcagtcct ggtcgcggca gtgccgccga tcatgctcaa gtcgccagcc aatccagagg
      901 gcctgccgat ggaagtcttc gaccagattc gtgcgggtgt cgccggggac cggtcccagt
      961 actataagga tctggccatc gccttctacg gcgccaaccg acccggagcg aaggtctccc
     1021 aaggcactct cgatcagttc tggctctgga gcatgcaggc cggcgcgaag aacgcctatg
     1081 agagcgtgaa ggcgttttcc gagaccgact tcaccgagga cctcaagaag ttcgacgtcc
     1141 ctaccctggt tttgcacgga gaggacgacc agatcgttcc ggtcaaggac tcggcgaaga
     1201 aatcggccaa gctcatcaag ggcgccaagg aaatctacta tcccggcgcg ccgcacggcc
     1261 tgacggcgac gcaccaggaa cgggtcaatg ccgacctgct cgccttcatc gaggggtagc
     1321 ggaaggacaa ggtcactttg agcggaaggc agaaaatgaa ggttttcgcc gtcggaggca
     1381 ccggccttat cggttcgacg tggagcctca ttcgcggctt ggcgatgtgg caacgggcgg
     1441 tcaggaggag acgaacatga gcttggacaa caccccacac ccctcgagca agcactgcgc
     1501 cgacacggtg cacgcgagat acacccacgg tagaccggag cacgagttgg ttccgtcgcg
     1561 ctacgcgctg aaggtcggcg cgattgacgt aatggtggtc agcgatgggg tgctatcgct
     1621 gccaggcgcg atgttgggcc acaacgtcga cccggccatc cgggcggcct ggctggagga
     1681 catgttcctg ccgcccgacg tgcttgagtg ggcgctgaac gcggtcgtgg tgcgtagcgg
     1741 tggccggacc atactcgtcg acgctgggat aggggcggag ttcccggact tgccgcgggc
     1801 cgggcagtgg gcccagcgac tggaggccgc cggcatcgat cccgcatccg tgaccgacgt
     1861 ggtgctcacc cacctgcaca tggaccactg cggcgggctg ctcgccgacg gggtgaaaga
     1921 gcggctgcgt ccggacctgc gggtccacgt ggcggccgcc gaggccgcgt tctgggagtg
     1981 gcccgatttc tcccgcgtct ccatgccgcc ggggttcccg gacgcgcttc ggcggacggg
     2041 caagcggttc ttgaaagagt tcagcagcca gctgcggacg ttcgagaacg agtatgaggt
     2101 ggcgccgggg gtagttgtcc aacgcaccgg cggccacacc cccgggcaca gcgtggtccg
     2161 cctggcgtcc ggcggcgacc ggctgacgtt cgctggagac gccgtgttcc aggtcgggt
//
LOCUS       DASRJQ010000165         2805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3832591, whole genome shotgun sequence.
ACCESSION   DASRJQ010000165 DASRJQ010000000
VERSION     DASRJQ010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3832591"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VFB89_03765"
     CDS             complement(<1..613)
                     /locus_tag="VFB89_03765"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF014873.4"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HZM26446.1"
                     /translation="MAELTIRPEEIREALDRFVRSYEPETATREETGTVVTSGDGIAR
                     VEGLPSAMANELLEFENGTLGIALNLDVREIGVVVMGESGGIEEGQVVKRTGDILSVP
                     VGDGYLGRTVDALGNPIDGLGEITPIDERRPLELQAAGVMDRQGVNQPLQTGIKAIDA
                     MIPIGRGQRQLIIGDRKTGKTAIAVDTIMNQKSAWDSGDPEQQV"
     gene            complement(680..1474)
                     /locus_tag="VFB89_03770"
     CDS             complement(680..1474)
                     /locus_tag="VFB89_03770"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HZM26447.1"
                     /translation="MTVATDARVSSLDRMLNDRQVGDSFATDMFAVVDALEASPTLRR
                     AVTDPGTPESARRALVHGLLDGKVDKAVVDLVGEAATLRWAGGRTFAAALERQAVRGQ
                     LVAADRRGELEDAEDQLFRFGRFVESDPDLRNVLADRAIDRSRRQELVGELLRGRATD
                     STIVLAKRAVAARERTFAQTIEGYVTLAAAQKNRVVATVRVAKPLTQDQRDRLVAALT
                     NQIGRQVTIQEVIDPDILGGVRVELGDEVFEGTVSGRLEAARRLFE"
     gene            complement(1471..2031)
                     /locus_tag="VFB89_03775"
     CDS             complement(1471..2031)
                     /locus_tag="VFB89_03775"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004412.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HZM26448.1"
                     /translation="MMTALLVPLQAEAENPLIPHLSEVIAAIVFALLLTYLISRFVVP
                     RFEATYAERTAAIQGGLERAEKAQEAADAALRQYNEQLAEARTEAARIREDAKSEGTQ
                     ILAELREQAQAEVARIRAQGEAQLDAERASVMTQMRAEIGTLATTLASRIVGESLEDD
                     ERARRTVDRFLAELEGEGEPTPAGRS"
     gene            complement(2042..2269)
                     /gene="atpE"
                     /locus_tag="VFB89_03780"
     CDS             complement(2042..2269)
                     /gene="atpE"
                     /locus_tag="VFB89_03780"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002526735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HZM26449.1"
                     /translation="MTPMELVGSLGIVGYGLAAIGPGIGVGLIFGSVIQGTARQPEAR
                     GAMMGTAWIGFAVTEALAIIGIALAFVFGGL"
     gene            complement(2324..>2805)
                     /gene="atpB"
                     /locus_tag="VFB89_03785"
     CDS             complement(2324..>2805)
                     /gene="atpB"
                     /locus_tag="VFB89_03785"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HZM26450.1"
                     /translation="VNNLFGVFPLSLLPTPSHIGWPYGLALLVWVIYNGVGIAKHGFF
                     GYLKHATLPSGVPAFMWPLIIPLEFVSNILVRPVTLSLRLFANMFAGHLLVLVFVLGG
                     EYLLLHSESIINKTAGVVALIFSMAIFGLEIFVQSLQAFIFTVLTAQYISSAAAEEH"
BASE COUNT          518 a          933 c          839 g          515 t
ORIGIN      
        1 gcacctgctg ctccgggtca ccgctgtccc aggcgctctt ctggttcatg atcgtgtcga
       61 cggcgatcgc agtcttcccg gtcttgcggt cgccgatgat caactgccgc tggccacgac
      121 cgatcgggat catcgcgtca atggccttga tccctgtctg cagtggctga ttgacaccct
      181 gccgatccat cactccggcg gcctgcagct cgagcggacg acgctcatcg atgggggtga
      241 tctcgccaag cccgtcaatc ggattgccca atgcgtcgac cgtacggccg aggtacccat
      301 caccgaccgg cacagaaagg atgtcaccag tgcgcttgac gacctggcct tcctcgatgc
      361 ccccggactc ccccatgacg acgacaccga tctcgcggac gtcaaggttc agcgcgatac
      421 caagagtgcc gttctcgaac tcgaggagct cattggccat cgccgaaggc aggccttcga
      481 cgcgcgcgat gccgtcgccg gatgtgacca ccgttccggt ctcttcacgg gtcgccgtct
      541 ccggctcgta gctccggaca aagcgatcca gtgcttcgcg gatttcctcg ggacgaatcg
      601 tcagttctgc catcgtctgc gctttctgct cttgtcgctg ggtgagtgtg ttcagttgtt
      661 ctcgactgta cgagccgtgc tattcaaaga gccgtcgcgc tgcctcgagc cggcccgaga
      721 cggtaccttc aaacacctcg tcgccgagct ctacgcgaac tccgcccaag atgtcgggat
      781 cgatgacctc ctggattgtc acctggcggc cgatctgatt ggtgagagcc gcaacgagcc
      841 ggtcccgctg gtcctgggtg agtggcttgg cgacgcgcac ggttgccacg actcgattct
      901 tctgtgctgc ggccagcgtg acgtaaccct cgattgtctg tgcaaaggtc cgctcacgag
      961 cggccaccgc ccgcttggca agcacgatcg tcgagtctgt ggcacggcct ctcagcagct
     1021 caccgaccag ctcctgccgc ctactgcggt cgatcgcgcg gtccgccagc acgttgcgca
     1081 gatccgggtc agactccaca aaccgaccga atcggaagag ctgatcctca gcgtcctcca
     1141 gctcaccacg tctatctgcc gccacgagct gaccacgcac ggcctgccgc tccagtgcag
     1201 ccgcgaacgt ccgaccccca gcccaacgca gcgtcgccgc ctcccccaca agatcaacaa
     1261 cggccttgtc gaccttgcca tcgagcaatc catgcaccaa cgcccgacgg gcactctctg
     1321 gcgttcccgg gtcggtcacc gcgcgtcgca gcgttggcga agcctccagc gcatcaacca
     1381 ccgcaaacat gtcggtagcg aagctgtcgc cgacctgcct gtcgttgagc atgcggtcga
     1441 gcgagctcac gcgcgcgtcg gtggctactg tcatgatctt ccggcaggtg ttggctcgcc
     1501 ttcaccttcg agctcggcga ggaagcgatc gaccgtacgc cgtgctcgct catcgtcctc
     1561 gagcgactca cccacgatcc ggctcgccaa tgtggtggcc aatgttccga tttcagctcg
     1621 catctgtgtc attaccgagg cgcgctcagc gtcgagttgg gcctctccct gggccctgat
     1681 ccgagcgacc tccgcttgtg cctgttcgcg gagctcagcg agaatctggg tgccctccga
     1741 cttggcgtcc tcgcgaatcc tggcggcttc agtgcgcgcc tcggcaagct gctcgttgta
     1801 ttggcgcaac gccgcatcgg cagcctcctg agccttctcc gccctctcca aaccaccctg
     1861 gatggcagcg gtgcgctcgg cgtaggtggc ctcgaaccgc ggcaccacga acctgctgat
     1921 gaggtaagtc agtagcagcg cgaacacgat cgcggcaatc acttccgaca gatgcgggat
     1981 aagcggattc tccgcctccg cctgaagagg cacgagaagc gcggtcatca tgaccactcg
     2041 ctcacaggcc gccgaagacg aaggcgaggg cgatgccgat gatcgccagc gcctcggtca
     2101 ccgcgaatcc gatccaggcc gtgcccatca tcgcgccgcg ggcctcgggc tgccgcgccg
     2161 taccctggat gaccgagccg aagatcaagc ccacgccgat tccggggccg atggcggcga
     2221 ggccgtagcc gacgatgccg agggatccga cgagttccat tggagtcatt tgcgacttct
     2281 ttcttgacgg ggtttcgcgg aatctgcgga aaccgagggt tggtcagtgc tcctcggccg
     2341 cggccgagga gatgtactgg gcggtgagaa cggtgaagat aaaagcttgc agggactgaa
     2401 cgaagatctc caggccgaag atcgccatgc tgaagatcag ggcgaccaca ccggcggtct
     2461 tgttgatgat cgactcggaa tgcagaagca ggtactcccc gcccaatacg aacaccagca
     2521 ccagcaggtg cccggcgaac atattggcga aaagtcgcaa ggacagggtg accggccgga
     2581 cgaggatgtt ggacacgaac tccagcggga tgatcagcgg ccacatgaat gcgggcacac
     2641 ctgagggcag cgtcgcgtgc ttgaggtaac caaagaaacc gtgcttggcg atgccaacgc
     2701 cgttatagat cacccacacc agaagcgcca ggccgtacgg ccaacctatg tgcgagggcg
     2761 tgggtagcag gctgagtggg aagactccaa ataagttgtt gacca
//
LOCUS       DASRJQ010000166         2029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2987091, whole genome shotgun sequence.
ACCESSION   DASRJQ010000166 DASRJQ010000000
VERSION     DASRJQ010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2987091"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VFB89_03790"
     CDS             complement(<1..536)
                     /locus_tag="VFB89_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26451.1"
                     /translation="MNETPDSDRSLNVELPPDESPRPVRPVRDIWDKVQILVQPLGGL
                     FTAFAIALLGFKSSEFLNRRQAIETNTRLYSELMSKREESESALRKDMFQTIIGSFVH
                     ASGTGDLNASVLNLELLAYNFHESLNLKPLFLDLKRRTSLQIAQARNPSVRAEYQGYM
                     DRLERVAREIARKQVIVL"
     gene            complement(533..880)
                     /locus_tag="VFB89_03795"
     CDS             complement(533..880)
                     /locus_tag="VFB89_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26452.1"
                     /translation="MRSMVVIFLIMGIAAVSATNVSAGKIFGDIKIDGKPLPEGVKVR
                     VTRPGTSAIADSTATDKFGSYKLLVKDTGKSVLTLVYDKKTLELPVFSNKEATRYDLI
                     LEKKDGNLILRRK"
     gene            1101..>2029
                     /locus_tag="VFB89_03800"
     CDS             1101..>2029
                     /locus_tag="VFB89_03800"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZM26453.1"
                     /translation="MRRSLRELLLGGPRNFSDPQIHRHLALIAFFAWVGLGSDGLSSS
                     CYGPEEIFRQLGTHRHLALYLVVALMGTVFLISASYSQIIEQFPSGGGGYLVATKLLG
                     PLPGVISGCALVVDYILTVAISVAAGCDAIFSFVSPSWQGLKLPAELAVVGGLIVLNL
                     RGIKESVYVLTPIFLAFIVSHVGLIAFGVLRHSADLGGLFAGTVGETRVAVGELGWLG
                     VGVIMLRAFSLGGGTFTGIEAVSNGVGILREPRVVNGKRTMLYMAASLAFTAGGILLC
                     YVLNQVQFETGKTLNASLWELLTQGWRLGTVEVG"
BASE COUNT          313 a          625 c          678 g          413 t
ORIGIN      
        1 tcgagcacga tcacctgctt gcgggcgatc tcgcgagcga ctcgctcgag acgatccatg
       61 tagccctggt actcggctcg cacggatgga ttgcgggctt gcgcgatctg gagcgaggtg
      121 cggcgcttga ggtccaggaa cagtggcttc aagttgagtg actcgtggaa gttgtaggcc
      181 agcagctcga gattgagcac gctcgcgttg aggtcgcccg tccccgacgc gtggacaaag
      241 ctcccgatga tggtttggaa catgtccttg cgcagggcgc tctccgattc ctcgcgcttg
      301 ctcatgagct cggaatagag ccgcgtgttc gtctcgatgg cctggcggcg gttgaggaac
      361 tcggaggact tgaagcccag gagtgcgatc gcgaaggccg taaacagtcc gccgagcggc
      421 tggacgagga tctggacctt gtcccagatg tcgcgcacgg ggcggaccgg ccgcggtgat
      481 tcgtcgggcg gcaactcgac gttcagtgat cgatcggagt cgggggtctc gttcacttgc
      541 ggcgcaggat cagattgccg tctttcttct cgaggatgag gtcatagcgc gtcgcctctt
      601 tgttcgagaa gaccggcagc tcgagcgtct tcttgtcgta aacgagcgtg agcacggact
      661 tgcccgtgtc cttgaccagc agcttgtagg acccgaattt gtccgtcgct gtcgaatcgg
      721 cgatggcgct cgtgcccggg cgcgtgacac gcaccttcac tccctcgggg agcggtttcc
      781 catcgatctt gatgtcgcca aaaatcttgc cggctgagac gttggtagca gacacggccg
      841 cgattcccat gatcaggaag atgacaacca tcgaacgcat gacactttcc ctcgagtggc
      901 gtcgagcgtg aaccaatgcg agcgcagtgt atcccttcgc gggggcagcc gcactacggc
      961 cgcgggcggg cggggctgtc ttgacgcttt cttgatgccc tcgaagggac tccatacgcc
     1021 ctcttgatcg cgcgccggcg tccaatcgga cgcatgaacg acgccgatag ggccgcctgg
     1081 gcacgcgcag ggctccgcag gtgagacggt ctctccgtga gctgctgctc ggtggaccgc
     1141 ggaacttctc ggatcctcaa atccaccgcc acctggcctt gatcgccttc ttcgcctggg
     1201 tgggactggg ctccgacgga ctctcctcgt cgtgctacgg acccgaggag atcttccgtc
     1261 agctcggcac tcaccggcac ctggcgctct atctggtcgt ggccctcatg ggcaccgtct
     1321 tcctgatctc cgcttcgtac agccagatca tcgagcagtt cccctcgggc ggcggcggct
     1381 acctggtggc gacaaaactg ctcgggccgt tgccgggggt gatctcaggg tgcgcgctgg
     1441 tggtggacta catcctcacc gtcgcgatct ccgtcgccgc cgggtgcgac gcgatcttct
     1501 cgttcgtctc cccgtcgtgg caggggctca agctgcccgc cgagctggcg gtggtgggcg
     1561 ggctcatcgt gctcaacctg cgcggcatca aggaatcggt ttacgtcctg accccgatct
     1621 tcctggcctt catcgtcagt cacgtcggcc tgatcgcttt cggagtgctt cgtcacagcg
     1681 ccgatctcgg cgggctgttc gcggggacgg tgggggagac aagagtcgcc gtcggcgagc
     1741 tggggtggct cggggtgggg gtgatcatgc tgcgcgcatt ctcgctcggc ggcggcacgt
     1801 tcaccggaat cgaagcggtc agcaacggtg tcgggatcct gcgtgagccg cgggtcgtca
     1861 acggcaagcg caccatgctg tacatggcgg cgtcgctcgc tttcaccgcc ggcgggatcc
     1921 tgctctgcta cgtgctcaac caggtccagt tcgaaaccgg caagacgctc aacgcctcgc
     1981 tgtgggagct gctcacccag ggctggcggc tcggcacggt ggaggtggg
//
LOCUS       DASRJQ010000167         5294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2510626, whole genome shotgun sequence.
ACCESSION   DASRJQ010000167 DASRJQ010000000
VERSION     DASRJQ010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5294
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2510626"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="VFB89_03805"
     CDS             complement(<1..1169)
                     /locus_tag="VFB89_03805"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HZM26454.1"
                     /translation="MGHPTRLLLGVSGFLALSGPLVAQEGVLRGGISTSDGRPVRGAT
                     VAVVGSHLIAFSDPSGEFVVLHVPVGPQQLRVTAIGFRPTTIDVTVDAVPAAPIRVAL
                     EQLASVLGDIIVQAPSRIPERVVEAPAAVSVTDMAAIPAVATTGQLPMALAAHPGIDL
                     AETDVHDFNVNARGFNSSLTRRVLVVQDGRDLAIPVLGSQEWSGLSLPLDDIARIEMV
                     RGPGSALYGANAFSGVLSITTPAAREVVGTKVSAGGGDPSVLRGDLRHAGLLAGGRIG
                     YRLNVGYTRNPLWAISRTQPGDLAREYAEAIDTVKYPVVHPVPGFEVLPLRGQTLSNG
                     LGSAATGSADHEEDVYGGARFDYYAPNGSVFTAEAGGAHATNNLFVTPVGRVQVNA"
     gene            complement(1214..4183)
                     /locus_tag="VFB89_03810"
     CDS             complement(1214..4183)
                     /locus_tag="VFB89_03810"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZM26455.1"
                     /translation="MLAPKSFEVLRYLVEHAGRLVTQRELLDSVWPGAFIEPQAVKKQ
                     IFDLRRVLDDPPRTPRFIETVPRRGYRFIATVEDSALRNPTVTAPPLTSRLVGRDGEL
                     AELRHCLRNALGGERQIVFITGEPGIGKTALTEEFQRQTAAREPSVRIARGQCVEGFG
                     SKEPFYPMIEALGQLCRGAEGATVLEILAKEAPTWLVQFAGLLRRDQRESLQREVLGA
                     TPERMLRELVKALETIATSRPLLLVFEDLHWVDYSTVDLLSGIARHRTSATLMLLATY
                     RPLDVALSDHPLAALKPDLLARQFCHEIAVPPLSRPEVVQYLGADPSAAEVPAGLAAL
                     VHRHSEGNPLFMIAVLEHLSARGILKRTDRAWELRRGLSSIDLEIPESLRQVIETQVA
                     RLSPHDQRVLEVASIAGVTFTPWVSAATGNLDPEDFEERCEALARRHHLLRVADTEAM
                     PGGDIAQRYEFQHALYREVLYRRQAPARRAALHRRLGERLVGLYGPKQEDIAQALAHH
                     FEQGFDWQHAVKYLRMAADTAERRFAPREATANLQHALELAGKLADSERAAAETEILE
                     ELAGFYTIAFDMRVIETYEALRERAAHYGLIDVEVRALIGMAYPVSWGSTRKCLEVVD
                     EALRMCSAQADPLTRARTRASCLVRRVWASGWNEMDAKECLEALEEIRRNGDRSVAAL
                     HTMECNFLYWVSSRYREARQDAVESLATLTQSHQDHGYRRFAHWLGQFTLPWSLLFLG
                     EWGEALRELDTEIAMATKNGDHYRGQTLSLYRAWVQDFAMDYAGARDLCDALLHILGD
                     VARRPWRRFALTFAGWAEVGLGNHERALEHLLAVKQEMDSHTVINDWYIRIMVQAAFT
                     ELRLGQGDLPRAQAEGEAFLAVACATADRTWHALAWEANARIALARLDLERAQGCITK
                     AFSAMEGYETPLAEWRVHATATELSERRGDSGVAKQHNALSRAAILKLADSLPGDDPL
                     RKTFLSAPRISTILAGG"
     gene            complement(4492..>5294)
                     /locus_tag="VFB89_03815"
     CDS             complement(4492..>5294)
                     /locus_tag="VFB89_03815"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HZM26456.1"
                     /translation="PGELVESELFGYVGGAFSGARREGCPGKFEAADGGTIFLDEIGE
                     LPPAAQISLLRVLQEGEITRVGCSQAKPVDVRVIAATNRDLQAALADGSLRRDLYYRL
                     NVLTLELPPLRERREDIPLLAGHFLETSSTAMRKRGLTFDAEVISALQGYDWPGNVRE
                     LQNVVARLVALASSSRITLADLPASIGASPPSPAPLPVPDSPRQSHRSRRSVSELEVA
                     EERAELLTLIKSARTMSEIAAQLGITRSTLYRRLERLGLQPRRILAAL"
BASE COUNT          918 a         1771 c         1711 g          894 t
ORIGIN      
        1 gcgttcacct ggacccggcc taccggggtc acgaacagat tgttcgtggc gtgggctccg
       61 cccgcctccg ccgtaaacac ggagccgttg ggcgcgtaat agtcgaaacg cgcgccgccg
      121 tacacgtcct cctcgtggtc cgcacttccg gtcgccgcac tcccgagacc gttcgacagt
      181 gtctggccgc ggagcgggag aacctcgaag cccgggacgg gatgcaccac cggatacttg
      241 acggtgtcta tggcctcggc gtactcgcgg gcgagatcgc caggctgcgt ccgcgaaatc
      301 gcccacaacg ggttgcgggt gtacccgacg ttgagccggt aaccgatgcg tccgcccgcg
      361 agcaggccgg cgtgccgcag gtcgccccgc agcacgcttg gatcaccacc gccggcgctc
      421 accttcgtgc ccaccacctc gcgcgccgcg ggcgtggtga tgctcagcac gccgctgaac
      481 gcattggcgc cgtacagcgc cgacccgggc ccgcgcacca tttcgatccg cgcgatgtcg
      541 tccagcggca gtgagagccc ggaccattcc tgcgagccga gaacgggaat ggccaggtca
      601 cgaccgtcct gcacgaccag cacacggcgg gtgagggacg aattgaaacc gcgggcgttg
      661 acgttgaaat cgtgcacgtc ggtttccgcg agatcgatgc cgggatgagc ggcgagcgcc
      721 atcgggagct ggcccgtcgt ggcgacggcg ggtatggcgg ccatgtccgt gaccgatacg
      781 gcggcggggg cctcgacgac ccgctccggt atccgggacg gtgcctggac gatgatgtcg
      841 cccagcacgc tggccagctg ctcgagggcc acacggatcg gcgcggctgg gaccgcgtct
      901 accgtgacgt cgatcgtcgt gggccgaaag ccgatcgccg tgaccctgag ctgctgcggc
      961 cccaccggga catgcagcac gacgaactcg ccggacggat cggagaaggc gatcaggtgc
     1021 gatccgacga ccgcaaccgt tgctccgcga accggtcggc catcggaggt gctgatcccg
     1081 ccgcgcagca cgccttcctg agccaccagg ggcccagaga gtgccaggaa tccggacacg
     1141 ccgagcaaca acctggtcgg gtggcccaaa ggcgtctcgt tgtatgtggc aagacctaaa
     1201 gcagccgcgc gttctagccc ccggcgagga tcgtcgagat gcgtggagcc gagagaaatg
     1261 tcttccgaag cgggtcatcg cctggcagcg aatccgccag cttcagaatg gcggcgcgac
     1321 tcagcgcgtt atgctgctta gcgacgccgc tgtccccccg gcgctccgag agctcggtag
     1381 ccgtcgcatg cacccgccac tcggcgagcg gggtttcgta gccttccatc gctgagaacg
     1441 ccttcgtgat gcagccctgc gcccgctcca gatcgaggcg cgcgagcgcg attctggcat
     1501 ttgcctccca ggccagcgca tgccaggtgc ggtcggcagt ggcgcatgca accgccagga
     1561 aggcttctcc ctctgcctgc gcgcgcggca gatctccctg tcccagccgc agctcagtga
     1621 aggcggcctg caccatgatc cgaatgtacc aatcgttgat caccgtgtgg ctatccatct
     1681 cctgcttcac tgccaacagg tgctccaggg cacgctcatg gttgcccagg cccacttcgg
     1741 cccacccggc gaaggtgagc gcaaaccgcc gccacggcct gcgtgccacg tctccaagga
     1801 tgtggaggag cgcgtcgcag agatcccgcg cacctgcgta gtccatcgcg aagtcctgaa
     1861 cccaggcccg gtagagcgac aaggtttgcc cgcggtagtg atcgccgttc ttcgtcgcca
     1921 tcgcaatctc ggtgtccagt tcgcgcagcg cctctcccca ttctcccagg aagaggagac
     1981 tccagggcag ggtaaactgc cccagccaat gggcgaagcg ccggtagccg tggtcctggt
     2041 ggctctgcgt gagggtcgcg aggctttcga cggcgtcttg tcgcgcttcg cggtaccggg
     2101 acgacaccca gtagagaaag ttgcactcca tggtatgcag cgccgcaacg gagcggtcac
     2161 cgttgcgccg aatctcctcc agcgcctcga gacactcttt cgcatccatc tcgttccacc
     2221 cgctggccca gactcgacgg accagacagc tcgcgcgagt ccgcgcacgc gtcaggggat
     2281 cagcttgggc cgagcacatc cgaagcgctt cgtctacaac ctcgaggcat tttcgcgtgc
     2341 tgccccacga cactgggtaa gccatcccga tgagcgcccg cacctccacg tcgatcagcc
     2401 cgtagtgggc agctcgctcg cgtagcgcct cgtacgtctc aatgaccctc atgtcgaaag
     2461 caatggtgta gaaacccgct agctcctcga gaatctcagt ctcggccgcg gcccgctccg
     2521 agtcggcgag cttgcctgcg agctcgagag catgctggag attggcggtc gcctcgcggg
     2581 gggcgaagcg ccgctcggcg gtatctgcgg ccatgcgcag gtatttgact gcgtgctgcc
     2641 agtcgaagcc ttgttcgaag tgatgcgcca gcgcctgcgc gatgtcctcc tgcttgggac
     2701 catatagccc taccagacgt tcgccgaggc ggcggtggag cgccgccctg cgggccggcg
     2761 cctggcgacg atagagcact tcacggtaga gcgcatgctg aaactcatag cgctgggcga
     2821 tgtcgccccc gggcatcgct tcggtgtccg cgacccgcaa caggtgatgc cgccgggcaa
     2881 gtgcctcgca gcgttcctcg aagtcctcgg gatccagatt cccagttgcc gcgctgaccc
     2941 aaggggtgaa ggtcacgcct gcgatgctgg ccacctcgag cacgcgctga tcatgtggac
     3001 tgagcctcgc aacttgggtc tcgatgacct ggcgcaggct ctccgggatc tccaagtcga
     3061 tactggagag gccacgtctc agctcccatg cccggtcagt tcgtttgagg atccctcgtg
     3121 cgctcaggtg ctcgagcaca gcgatcatga ataggggatt cccctcggag tggcggtgca
     3181 ccagggcggc aagcccggct ggaacctcgg ctgccgaggg atcggcgccg aggtactgaa
     3241 ctacctccgg cctactcaac ggcgggaccg ctatttcgtg acagaactgg cgtgcgagga
     3301 gatcgggttt aagtgccgcc agaggatggt ccgagagggc cacgtcaagc gggcggtagg
     3361 tcgcaagcag catgagcgtg gcggatgtcc ggtgccgcgc gatgcccgat aagagatcga
     3421 ctgtcgaata gtcgacccag tggagatcct cgaagacgag caggagcgga cggctggtcg
     3481 caatcgtctc taaggccttc acgagctccc gaagcatgcg ctcgggcgtg gcacccagga
     3541 cttcgcgctg gagcgactcg cgctggtcgc gcctcagcag cccggcgaac tgcaccaacc
     3601 aggttggcgc ttccttcgcg agaatctcca ggacggtagc gccttcggcc ccgcggcaca
     3661 gctggcctag tgcttcgatc atcggataga acggttcctt gctgccaaac ccctccacgc
     3721 attggccccg cgcgatccga acggacggtt cgcgggctgc cgtttgccgc tggaattcct
     3781 ccgttagcgc cgtctttccg ataccgggct cgccggtgat gaagacgatt tgccgctcgc
     3841 ctcccaaggc gtttctgagg cagtgccgca gctcggccag ctcgccgtcg cgcccgacca
     3901 aacggctggt gagcgggggc gctgtgacgg ttgggttgcg gagagcgctg tcttcgaccg
     3961 ttgcgatgaa ccggtagccc cgtctcggca ccgtctcgat gaagcgcggg gtccgcggcg
     4021 ggtcatcgag tacccgtcga aggtcgaaga tctgcttctt gactgcctgt ggttcgatga
     4081 aggcgcctgg ccagaccgaa tcgagcagct cgcgctgggt gaccagtcgg ccggcgtgct
     4141 ccaccaggta gcgcaggact tcgaagctct ttggcgccaa caagattcgc tcatctgccc
     4201 ccgcttcgcg ggagcgccag aggcactcgt tgacggtatc gagacggaag ggagggaaca
     4261 gcttcagggc gacgcacctc cagcggcacg cgttccggac cgacccacga ctccaacata
     4321 atctttccgc gcgagcattc cattagtgct gtgccgtcga taggcccttc agattctgcg
     4381 acaaatggag acgatgcgca gttagaaggc tcggcactgc ccaccttcgt ggccggactg
     4441 gagggatgta cctacctgga aggggaagtg ccggcctgaa cccagcgccc ctcagagagc
     4501 ggcgaggata cgcctgggct gtagcccgag gcgttccagc cgccggtaga gggtgctccg
     4561 ggtgataccg agctgtgcgg cgatttcact catggtgcgc gccgacttga tgagcgtcag
     4621 cagctccgcg cgctcctccg ccacttccaa ctcggataca gagcgcctcg atctgtggga
     4681 ctgtcgggga gaatccggaa ctgggagtgg agccgggctc ggaggactcg caccgatcga
     4741 cgcgggaaga tcggccagcg tgatgcggct ggaggacgcc agggccacca gccgggcgac
     4801 cacgttctgc agctcgcgca cgttgcccgg ccaatcatac ccctgcaggg cgctgatcac
     4861 ttccgcgtcg aaagtaagtc cccgtttccg catcgcggtc gaggacgtct cgaggaaatg
     4921 cccggccaac aacggaatgt cctctcgccg ctcccgtagc gggggcaact ccagggtgag
     4981 cacgttcagc cggtaataca gatcgcgccg aagcgagccg tcggccaggg ccgcctgcag
     5041 gtcgcgattg gtcgcggcga tcacccgcac atccaccggt ttagcctggg agcaaccgac
     5101 tcgggtgatc tccccctcct gcaacacccg gagcagtgag atttgtgcag ccggcggcag
     5161 ctctccgatc tcatccagga aaatcgttcc gccatctgct gcctcgaatt tgccgggaca
     5221 cccttcccgc cgcgcgcctg agaacgcccc accgacatag ccgaacagct cgctctcgac
     5281 caactccccc ggca
//
LOCUS       DASRJQ010000168         2936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2986429, whole genome shotgun sequence.
ACCESSION   DASRJQ010000168 DASRJQ010000000
VERSION     DASRJQ010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2986429"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2021)
                     /locus_tag="VFB89_03820"
     CDS             complement(<1..2021)
                     /locus_tag="VFB89_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZM26457.1"
                     /translation="MPTDTLTTEDAVRLAGIIFGLAAEVRRLDGEFDDNFLLLVAGQP
                     HSVLKVSRPEEQRAFLDLQAKVLGRLGRTPDTAAFQFPQLLPTRNATTLATVPGGCPG
                     AGRFIRLLSYIPGCRHLAEVVPCPPPLLTSLGTLLATVDRALLGLDDPAAHRTRNGSG
                     DDRFRWALADSLWVRDALTHVKDARRRALAGWVLDRVEREALPLLPALRHSVIHGDAS
                     EYNVLVRDDRAVALIDLGDLHWGPTVGELAVGSVYAMFGVGDPLGAAAYLVAGYNEVI
                     PLTQPERLALFPFILARLAVSVVNAALRNSAEPDNAYFTVSSAPGWAILEQLAEVPSR
                     VAEAMLFSRVDDRRPLPSVDTLRVTRQRALGPSLSLSYRTPLHIVRGWKQYLYDADGL
                     EYLDAYNNVAHVGHCHPHVVDAIARQAALLNTNTRYLHHLIGEYAERLTALVPAPLGV
                     CYFVNSGSEANELALRLARAHTRQRDMIVSTGAYHGNTQKLIDVSPYKYAGPGGEGRE
                     PWVHEVPLPDVYRGAYKASDPEAGAKYAQHVGQVVDEIQAAGRGVCAFLFESLPSVGG
                     QIIPPPGYLTSAYAAVRAAGGLCIADEVQVGFGRVGTHIWGFELQGVVPDIVVCGKPI
                     GNGHPLGAVITTPGIAASFANGMEYFNTFGGNPVSMAAGLAVLKVLAEEG"
     gene            2286..2729
                     /locus_tag="VFB89_03825"
     CDS             2286..2729
                     /locus_tag="VFB89_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26458.1"
                     /translation="MPRNPTMGAPMARKKAAKKAAKKRAPKKEPADLFASNDPVAAIA
                     AKLQTLEGQAARGDLAPSGLEAFKSALDELRLRAWGLLMATNADDPRGFQERFRIRRG
                     KDMCRALCRDLYEGKLSGQHPDLPELSAAAHDLAAAVDNARRQTS"
BASE COUNT          548 a          953 c          969 g          466 t
ORIGIN      
        1 ccttcctctg caaggacctt cagcacggcc agccccgccg ccatcgagac cgggtttccc
       61 ccgaaggtat tgaagtactc catgccatta gcaaagctcg ctgcgatccc tggcgtagtg
      121 atgaccgcgc ccagggggtg cccattgccg atgggctttc cgcacacgac gatgtccggc
      181 accacgccct gcagctcgaa gccccagata tgcgtcccca cccggccaaa gccgacctgg
      241 acctcgtctg cgatgcaaag tccaccagcc gctcgtacgg cggcgtacgc cgatgtgaga
      301 tagcccggag gtggaatgat ctggcccccc acggacggaa gactctcgaa caggaatgcg
      361 cagacgcccc gccccgccgc ttgaatctca tccaccactt gccccacgtg ctgcgcgtac
      421 ttcgccccgg cctcgggatc gctcgccttg tacgccccgc gatacacgtc cggcagcggc
      481 acctcgtgca cccagggctc gcgcccctcg ccgcctggcc ccgcgtactt gtacggggag
      541 acgtcgatta gcttctgcgt gttgccgtgg tacgcgccgg tactcacgat catgtcgcgc
      601 tggcgcgtgt gggcccgtgc caatctcaat gccagctcgt tggcctcgct tccggagttc
      661 acgaaatagc acaccccaag cggtgccggg accaatgccg tgagccgctc ggcgtactcg
      721 ccgatcagat gatggaggta ccgggtgttg gtgttgagca gcgccgcctg cctggcaatg
      781 gcgtccacta cgtgtgggtg gcagtggccc acgtgggcga cgttgttgta ggcatcgagg
      841 tactcgagtc cgtccgcatc gtagaggtac tgcttccagc cccgcacgat gtgcagcggc
      901 gtgcgatacg aaaggctcag actcgggcca agggcgcgct gtcgtgtaac gcggagcgta
      961 tcgaccgatg ggagcggcct tcggtcatcg acgcgcgaaa acagcatcgc ctcggcaact
     1021 cggctgggta cttcggccag ttgctccagg atggcccagc ccggggccga gctgaccgtg
     1081 aaatacgcgt tgtctggctc agcggagttg cgcagcgccg cgttgacgac gcttacggcg
     1141 agtcgagcca gaatgaaagg gaaaagcgcc agccgctccg gctgggtcag cgggatgacc
     1201 tcgttatagc cggcgaccag gtatgcggcc gcgccaagcg gatcgcccac gccgaacatg
     1261 gcatagaccg atcccacggc aagctcgcca accgtcgggc cccagtgcag gtcgcccagg
     1321 tcaatgagcg ccacagcacg atcgtcccgc accagcacgt tgtactcgct cgcgtcacca
     1381 tggatcactg aatggcgaag agctggcaag aggggcagcg cctcgcgctc gacccgatcg
     1441 agcacccagc ccgcaagcgc tcggcgcctc gcgtctttga catgagtcag agcatcgcgc
     1501 acccagagcg agtcggccag ggcccagcga aaccggtcgt cgccggagcc attgcgagtg
     1561 cggtgtgccg cggggtcgtc gagaccaagg agcgcccggt ccacggtggc gagcagtgtg
     1621 cccaggctgg tgagaagagg tggcgggcaa ggtaccactt cggccaggtg gcggcagccg
     1681 gggatgtaac tgaggaggcg aatgaaccgg cccgcccccg ggcaaccacc gggcaccgtg
     1741 gccagcgtcg tggcatttcg tgtcgggagc agctggggaa attggaacgc ggccgtgtcg
     1801 ggggtccgac cgagccggcc gagcactttg gcctgcagat cgagaaacgc gcgctgctct
     1861 tcgggccgtg agaccttgag aacgctgtgt ggctgaccgg cgaccaagag caggaagttg
     1921 tcatcgaact cgccatcgag ccgtcgtacc tcggccgcca ggccgaagat gatgccagcg
     1981 aggcgtactg cgtcctcggt ggtgagtgtg tcggtgggca tacaagaaag tagtcgtggc
     2041 atgggggctg cggatgaggg atgcgcgagt ggcatccaag aacgagtggg caccttcggt
     2101 ctcactgtaa gcgtgcaggt gagagtcgcg ggcggccatg ctcaaactta gaatccgcat
     2161 tgggctgccg cctaacccac gcttgcagct gccgggcgca cctctttcaa cggaccttag
     2221 aattcggaac tacgaggctc gtcgcccgca gctgaagcgg gactgttagg cgccagccaa
     2281 aagcgatgcc tcgcaatcca accatggggg ctccgatggc ccggaagaag gccgcgaaga
     2341 aggcggcaaa gaaacgagcg ccgaagaagg aacccgcgga cctgttcgcg tcgaacgacc
     2401 cggtcgcggc aatcgccgca aagctacaaa ctctggaggg gcaggctgct cggggcgacc
     2461 tggccccctc tgggctggag gcgttcaaga gtgccttgga cgagctgcgg ctccgggcct
     2521 ggggtctgct catggcaacc aacgcggatg acccccgggg attccaagag cgcttccgca
     2581 tccgtcgtgg gaaggacatg tgccgtgccc tctgccgtga tctttacgag ggcaaactga
     2641 gcggtcagca ccccgatcta cctgaactca gcgcagcggc gcacgacctt gcggcagcgg
     2701 tggacaacgc gaggcgccag acctcctgac gtccccagca cgcagccgtc tcgcagctgg
     2761 cgcctaacca ccgcttgccg ctggcgggcg cacggctacg cagagtgccg ttaggcttct
     2821 cggacacacg aacatcaatg agcgacgaca ccagtgtcgg ggtttgccag cgctacatgc
     2881 ggcgcgagct cacccttgaa gcggcgggaa gccagctgta tgacccggta cgcgcc
//
LOCUS       DASRJQ010000169         2966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2128896, whole genome shotgun sequence.
ACCESSION   DASRJQ010000169 DASRJQ010000000
VERSION     DASRJQ010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2128896"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VFB89_03830"
     CDS             <1..735
                     /locus_tag="VFB89_03830"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZM26459.1"
                     /translation="RTDRSKPHPILSDINVRRAIGLAIDRRPIVQAVFGQYAEVPYGP
                     ASQLLWIRKGAPKPAGPDTVQARRLLAKSGWYDRDGDGIVERDGKPLTLTLSFPLTSE
                     ARKQIALQVQEQLRHAGVRIDLARLEGPVWAQRRSQGDFDIDFSAATQDPTPSGLTQS
                     WSCRGGTNVGGYCDPGVDSLLDRAITMRSASAAIWQEILRRIESSQPAVFMYAPAFAY
                     AVNRRFTKAPIRPESSWLLIRDWTFR"
     gene            732..1703
                     /locus_tag="VFB89_03835"
     CDS             732..1703
                     /locus_tag="VFB89_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM26460.1"
                     /translation="MRRWLIRRGLQSVITLVIVAILLFFLMRLAPGDPLGQMSSERQL
                     TPAQIEATRKLYQLDQPLPRQFFNWLAALAHGNLGTSFTHGRPVTSLIAERLPATLLL
                     GGTVLLVNFTVGLWLGAAQAVLRGTPADRWLTALSLAGYAMPSFWLGLMLAWLGAVHW
                     HLFPAAGFENPLLAPDAPWWTRATDVLAHLVLPATTLVIVSIAGTMRYQRRAMLEVLP
                     LPYIVVARAKGLPERDVRRRHAWRNALFPVLTLVGLWLPILATGAVFVEAVFAWPGLG
                     SLAAEAIGHRDYPLLMGTALLVSGLIILGGLLTDVAYAVLDPRVRWA"
     gene            1694..2545
                     /locus_tag="VFB89_03840"
     CDS             1694..2545
                     /locus_tag="VFB89_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM26461.1"
                     /translation="MGVIDLVRELLAAVWRRPAGRIGLVMLGTVVIAALVAPPFLPDP
                     IAQPDIVGQASLPPGPGHLFGTDQLSRDVLSRVASGARISLAVAGLAVLLSVTIGAGV
                     GLTAGYLGGATDTVLMRLVDAALAVPRLFILLLLLAVWERIPLVALIIILGTTGWFGT
                     SRLVRGEVLRLRHEPFITGAEALGARRARVMFRHLFPNTVGPLLVAATLGVGEVILLE
                     AGLSFLGLGIQPPTPSWGGMILDAKPVLITAPWTSLFPGLAVVVTVLAVNLLGEALAG
                     AVDPRES"
     gene            2542..>2966
                     /locus_tag="VFB89_03845"
     CDS             2542..>2966
                     /locus_tag="VFB89_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZM26462.1"
                     /translation="MSRVLEVENLRVTFPGPNGQRVAAVDGVNFVLHRGETLALVGES
                     GCGKSLTSLSLLRLVPAPGRIEPESRIRLGTTSVLALEGDALREIRGRRIGMIFQDPM
                     TSLDPVFRVGDQIAEGIRAHFPISKRDARDRAVALLREVG"
BASE COUNT          419 a          986 c          964 g          597 t
ORIGIN      
        1 cgcacggacc gttcgaagcc gcatcccatc ctgagcgata tcaacgtgcg gcgtgcgatc
       61 ggactcgcga tcgatcggcg ccccattgtg caagcggtgt tcggccagta tgcggaagtc
      121 ccgtatggac cggcgtcgca attactctgg attcgcaagg gtgcaccgaa gccggccggc
      181 cccgacacgg ttcaggcgcg gcgactgctc gccaagagtg gctggtacga tcgcgatggg
      241 gacgggatcg tcgagcgcga cgggaagcca ctcacattga cgctgagctt cccactcacg
      301 agcgaagcgc gcaagcagat cgccctccag gtccaggagc agctccgaca tgctggcgtc
      361 aggatcgact tggcgcggct ggaaggtccg gtctgggctc agcgccggtc gcagggcgac
      421 ttcgacatcg acttctcggc cgccacgcag gatccgactc cctcgggcct cactcagagc
      481 tggtcgtgcc gtggaggaac caacgtcggc ggctactgcg atcccggcgt ggactcgctg
      541 ctcgaccggg cgatcacgat gcgctcggcc agcgccgcca tctggcagga aatcctgcgc
      601 cgcatcgaat cgtcgcagcc ggcagtgttc atgtacgccc ctgcctttgc ctacgcggtg
      661 aatcgccgct tcaccaaagc gcccatccgg ccggagtctt cctggctgct gattagggac
      721 tggaccttca ggtgagacgc tggctcatcc ggcgtggcct ccaatccgtc atcacgttgg
      781 tgatcgttgc catcctgctg ttctttctca tgcgacttgc gccaggagat ccgctgggcc
      841 agatgagcag tgagcgccag ctcacgccgg cgcagatcga ggcgaccagg aagctctatc
      901 aactggacca accgcttccc cgccagttct tcaactggct cgcggcgctc gcacacggga
      961 atctcggcac gtccttcact cacggccgtc ccgtcacgtc gttgatcgcc gaacggttgc
     1021 cggccacctt gctgctgggc ggcacggtac ttctcgtcaa cttcaccgtc ggcctatggc
     1081 tgggtgctgc gcaagcggtt ctccgcggca ctcccgccga ccgttggctc accgcactct
     1141 cgctggccgg ctacgcgatg ccgtcgttct ggcttggcct catgctggcg tggctgggtg
     1201 cggtgcattg gcacctgttt ccagcggcgg gattcgagaa cccattgctc gcgcccgacg
     1261 caccctggtg gaccagggcg acggacgtcc tcgcccacct cgtgctccct gctaccactc
     1321 tcgtgatcgt cagcatcgcc ggcaccatgc gctaccagcg ccgagccatg ctcgaggtgc
     1381 ttccccttcc ttatatagtg gtcgcccgtg ccaagggtct cccggagcgg gacgtccgtc
     1441 gccggcacgc atggcggaac gctctgtttc ccgtgctcac gctggtcgga ctgtggctgc
     1501 cgatcctggc caccggtgcg gtattcgtgg aggccgtgtt cgcctggccc ggcctgggct
     1561 cgctcgctgc tgaagcaatc ggtcatcgtg actatcccct tctgatgggc acggccttgc
     1621 tggtttcggg gctcatcatc ctgggggggc tgctgaccga cgtggcgtat gcggtgctcg
     1681 atccccgagt tcgatgggcg taatcgatct cgttcgcgag ttgctcgccg cagtgtggcg
     1741 ccgaccagcc ggccggatcg gactggtcat gctcgggacc gtggtgatcg cagcgctggt
     1801 cgcccctcct tttctgccgg atcccatcgc ccagcccgac atcgtcggtc aggcgagcct
     1861 gccgcccggc cccggccatc tgtttggaac cgatcagctg agtcgtgacg tgctctcgcg
     1921 agtcgcttcc ggcgcccgca tctccctcgc tgtcgcaggg ctcgctgtcc tcctgtcggt
     1981 cacgatcggc gcgggggtgg gattgaccgc cggctacctc ggcggtgcta ccgacacggt
     2041 gctgatgcgg ctggtcgatg cggcgttggc tgtcccgcgg cttttcatcc tgctgctcct
     2101 gctcgcggtg tgggagcgga ttcccctggt cgctctgatc atcattctgg gaacgaccgg
     2161 ctggttcggc accagtcgcc tggttcgggg tgaggtgctc cggcttcgtc acgaaccgtt
     2221 catcaccggc gccgaagctc tcggcgcccg tcgagcgcgg gttatgttcc ggcatctgtt
     2281 ccctaatacg gtgggaccgc tgctggtggc cgccacgctg ggcgtcggcg aagtgattct
     2341 gctcgaggcg ggactgtcgt ttctcggtct gggcattcag ccgccgacgc catcatgggg
     2401 tgggatgatt ctggacgcga agccggttct gatcactgct ccctggacca gtctctttcc
     2461 cggtctcgca gtcgtggtca ccgtgctcgc tgtcaatctg ctgggcgaag ccctcgcagg
     2521 cgcagtggat ccgagagaat cgtgagccga gttctggaag tcgagaatct gcgggtgacg
     2581 tttcccggac cgaacggtca gcgggtcgcg gcagtggacg gcgtcaactt cgtgctgcat
     2641 cggggcgaga ccctcgcgct ggtcggcgag tctggctgcg gaaagagtct caccagcctg
     2701 agcctcctcc gtctggtgcc ggctccgggg cggatcgagc cggagagccg tattcgcctg
     2761 ggcactacca gcgtgctcgc gctcgaaggc gatgcgctcc gcgaaatccg cggccgccgg
     2821 atcggaatga ttttccaaga ccccatgacg agcctcgacc cggtgttccg ggtgggcgat
     2881 cagatcgccg aaggcattcg ggcccacttt ccgatttcaa aacgggatgc tcgggatcgc
     2941 gcggtcgcac tgcttcggga agtcgg
//
LOCUS       DASRJQ010000170         7750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2171367, whole genome shotgun sequence.
ACCESSION   DASRJQ010000170 DASRJQ010000000
VERSION     DASRJQ010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7750
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2171367"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2452
                     /locus_tag="VFB89_03850"
     CDS             <1..2452
                     /locus_tag="VFB89_03850"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HZM26463.1"
                     /translation="PEQLVSIAAALIPFLEHDDANRALMGSNMQRQAVPLLFPQAPVV
                     GTGLEDKVARDSGAVIVARRAGRVTRVTADEIMIDAGVDGPTDDGQPLARLHQYDRYR
                     VRKFWRTNQDTAINQRPLVQVGQRVAAGEVIADGAGTDHGELALGSNVLVAFMPWYGH
                     NFEDAIVLSERLVKDDVYSSIHIQELELHVRDTKRGQEEITREIPNVSDESVVDLDER
                     GIVRIGAHVKPGDILVGKITPKGETELSPEEKLLTAIFGEKAKDVKDSSLKVPPGMAG
                     VVIDVKIFSRIEDQVVEKDRGERIGEVRRIETDEKARISTVMMDELARLLEGQEVALM
                     LRSGTVEEFRPAGTKLTKPMLEEIDLGDVDLKTLRVANRAANERIRAAIDAAARERAK
                     IEEKAEDQIDKILQPDELPPGVIQLVKVYIAEKRKISVGDKMAGRHGNKGIVARIVPE
                     EDMPFLPDGTPVDIVLNPLGVPSRMNVGQILETHLGWCARILGFEAKTPVFRGADEGE
                     IGTLLRLAGFKWAAEGLRLRARPPEFELETIARTVQDLKKVAPNGERTTLLQAGIDVL
                     GARGASAETRALHHELRKFLVESARELAEREVEQRKHEGEFHQAHEADHAGQKAWTRA
                     VKEIEKATQASPAEALETAGKPALAALLAHQADASLDAAADELLRAAGLTPAGKARLR
                     DGRTGQLFEGDVTVGSIYMLKLSHLVDDKIHARSIGPYSLVTQQPLAGKAQFGGQRFG
                     EMEVWALEAYGAAHVLQEMLTVKSDDVNGRSKVYEAIVKGQNLPEPGIPESFNVLVKE
                     LQALGLKVTLGTTESGDE"
     gene            2463..6983
                     /gene="rpoC"
                     /locus_tag="VFB89_03855"
     CDS             2463..6983
                     /gene="rpoC"
                     /locus_tag="VFB89_03855"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HZM26464.1"
                     /translation="MIDFRSGREPKSSSFDYISIRIASPEEIRGPRDPKERERLELQG
                     QRTWWSWGEVTKPETINYRSFKPEKDGLFCERIFGPVKDWECHCGKYKRIRYRGVICD
                     RCGVEVTLSKVRRERMGHIELSVPVAHIWFFKTLPSPMGNLINITLRDLERVIYYSSY
                     IVIEPGDQEVEASQLLDEEEYLTLRQKARDEGDTGFRADIGAPAVRALLERLDVKKLA
                     EELRAGVATETSQHRKKQMLKRLKIVDAFLSSGDSGDLPNIPAWMILDVVPVIPPDLR
                     PLVPLDGGRFATSDLNDLYRRVINRNNRLQKLIQHKAPEVILRNEKRMLQEAVDALFD
                     NGRRSKAIRGRGKRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELRLHQC
                     GLPKAMAVELFKPFIIHKLVEKGIAETVKRAKKIVERESPEVYEILEEIIQDHPVLLN
                     RAPTLHRLGIQAFEPVLVEGKAIRIHPLVCAAFNADFDGDQMAVHVPLSFEAQLEARL
                     LMISSNNILKPSDGRPVAEPSQDMVLGSYFLTKDPAGFEEELRRAGAGKKEETEKRLW
                     KDAKHFGSLAEIEMALLAGAVKYHTPIHFWYVPPRETVDEEMRRPRWVRTTVGRVLFN
                     SIVPKAVIVERGFRNYVMKKKQLSELVLEAYRRAGLSDTVLFLDRLKEFGFGFATRGG
                     VSIGIEDLEIPTEKADLLNDADGRVKRFQRAYNTGQITFGERYNKVIDAWTHANNDVA
                     EAMVNHMRQSRGGFNPVYMMFDSGSRGSRDQIRQLAGMRGLMAKPQKKLTGGIGEIIE
                     SPIKSNFREGLTVLEYFISTHGARKGLADTALKTADAGYLTRRLVDVAQDVTITEEDC
                     GTILGLEIAALKEGEDIIEPLRERIVGTVASEDVFDPHELDEHGDPQLLVQAGQLIDE
                     EIADDIDDSGIEKVKIRSVLTCEARRGICRMCYGRNLATMDMVDMGEAVGILAAQSIG
                     EPGTQLTLRTFHIGGTSSRIAEEAERRARSDGRVQYTEGLEWTDLPVEYEDGTNATIR
                     VVLTREDESAAEEHKEGILVVDPKDPKRVLNRYPVPEGALLAVREGDTVTKGDNDRRA
                     TILYTWDPYNDPSIIKSDGELRWKDLVPGVTLREELDEGTGLRSIVVMADPDRELHPS
                     VLVYTSHRKDPQEYTLAEGSRIILGSPTDQVSRAMEIPDEANPWSTKLHVDERPINEA
                     WPSKSKKESKDKTVFLSTPVKVAKGVTVTKIPRQAYKTRDITGGLPRVAELFEARRPK
                     DPATISEVDGVVKFGEIKRGKREIYVYPDGTDSETPAQLYEVPAGKHLRVHEGDRVRA
                     GDRLTEGPVNPHDILLIKGPRAVQEYLLNEVQEVYRLQGVRIADKHIGVIVRQMLQKV
                     RVTDPGDTDFLEGETVDRLSFRDENDRTLRRKGTPAQAEPVLLGITKASLTTQSFISA
                     ASFQETTRVLTDAAIRGAKDDLLGLKENIIIGHLIPAGTGLSRYRKVEVIGDEEDEVE
                     KASPVEEEVKTA"
     gene            7052..7429
                     /gene="rpsL"
                     /locus_tag="VFB89_03860"
     CDS             7052..7429
                     /gene="rpsL"
                     /locus_tag="VFB89_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HZM26465.1"
                     /translation="MPTLNQLVRHGRRRQLSKTASPALKAAPQKRGVCTRVYTSTPKK
                     PNSALRKVARVRLTNGMEVTCYIPGEGHNLQEHSIVLIRGGRVKDLPGVRYHVVRGTL
                     DASGVDGRQQSRSKYGTKRKKGA"
     gene            7433..>7750
                     /locus_tag="VFB89_03865"
     CDS             7433..>7750
                     /locus_tag="VFB89_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012404.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HZM26466.1"
                     /translation="MRRAAKIHRIELQPDARYNNVLVTRFINCLMYAGKKSTAERIFY
                     GAMDVIEKKTGQDGLTVLKAAMTNVKPVLEVKSRRVGGATYQVPVEVRQDRRTALALR
                     WLIQ"
BASE COUNT         1522 a         2345 c         2721 g         1162 t
ORIGIN      
        1 gccggagcag ctggtctcta tcgccgcggc attgattccg ttcttggagc acgacgacgc
       61 caaccgtgcg ctgatgggtt ccaacatgca gcgccaggcc gtcccgctcc tgtttcctca
      121 ggcccctgtg gtgggcaccg ggctcgaaga caaggtggcc cgcgactcgg gtgcggtgat
      181 tgtcgcgcgg cgcgcgggga gagtgacccg ggtgaccgcc gatgagatca tgatcgatgc
      241 cggagtggac ggcccgaccg acgacgggca gccgctcgcc cggctgcacc agtacgaccg
      301 gtaccgggtg cggaagttct ggcggaccaa ccaggacacg gcgatcaacc agaggccgct
      361 ggtacaggtg ggtcagcggg tcgccgcggg cgaagtcatt gccgatggcg ccggcaccga
      421 ccatggcgag ctggcgctcg gcagcaacgt gctggtggcg ttcatgccgt ggtacgggca
      481 caacttcgag gacgccatcg tcctgtcgga gcggctggtg aaggacgacg tctactcgtc
      541 gatccacatc caggagctcg agctgcacgt ccgcgacacc aagcggggcc aggaggagat
      601 cactcgggaa atccccaacg tctccgatga gagcgtggtg gatctcgacg agcgcggcat
      661 cgtgcggatc ggcgcacacg tcaagccggg tgacatcctg gtcggcaaga tcacgcccaa
      721 gggcgagacc gagctgtcac cggaagagaa gctgctgacg gccatcttcg gcgagaaggc
      781 caaagacgtg aaggacagct cgctcaaggt cccgcccggc atggccggtg tcgtgatcga
      841 cgtcaagatc ttcagccgga ttgaagatca ggtggtcgag aaggatcggg gcgagcggat
      901 cggcgaggtc cgccggatcg agaccgacga gaaggcccgg atcagcacgg tcatgatgga
      961 cgagctggct cgactgctcg agggccagga agtggcgctg atgctccggt cgggaacggt
     1021 cgaagagttt cgtcccgcgg gcaccaagct cacgaagccc atgctcgagg aaatcgacct
     1081 gggcgacgtg gacctcaaga ccctccgggt cgccaatcgg gcggccaacg agcggattcg
     1141 cgcggctatc gatgccgcag ctcgggagcg ggccaagatc gaggagaagg cggaagacca
     1201 gatcgacaag atcctccagc cggacgagct cccgccggga gtgatccagc tggtgaaggt
     1261 gtatatcgcc gagaagcgga agatctcggt gggcgacaag atggcgggcc ggcacggcaa
     1321 caagggcatc gtcgcccgga tcgtgccgga ggaagacatg ccgttcctgc cggacggcac
     1381 tccggtggac atcgtgctca atccgctcgg cgtgccgagc cggatgaacg tgggccagat
     1441 cttggagacc catctgggct ggtgcgcccg aatccttgga ttcgaggcca agacgccggt
     1501 gttccgtgga gccgacgagg gtgagatcgg aaccctgctg cggctggccg gtttcaagtg
     1561 ggctgccgag gggcttcgcc tgcgggctcg gccacccgag ttcgagctgg agaccatcgc
     1621 gcggacggtg caggacctga agaaggtcgc gccgaacggc gagcgcacca ccctgctcca
     1681 ggcaggaatc gacgtgcttg gcgccagggg cgcgtcggcc gagaccaggg cgctgcatca
     1741 cgagctgcgg aagttcctgg tggaatcggc tcgtgagctg gcggagcgcg aggtcgagca
     1801 gcggaagcac gaaggggagt tccaccaggc gcacgaggcc gaccacgccg ggcagaaggc
     1861 atggacccgc gcggtgaagg agatcgagaa ggccacccag gcctcaccgg ccgaggcgct
     1921 cgagaccgcg ggcaagccgg cattggcggc gctgctcgcg catcaggcgg acgccagtct
     1981 cgacgcggcc gccgacgaac tcctccgcgc ggccgggctc actccggccg gaaaggcgcg
     2041 gcttcgtgac ggacgcacgg gccagctgtt cgagggcgac gtgacggtag gctcgatcta
     2101 catgctgaag ctgagccacc tggtggacga caagatccac gcccggtcga ttggaccgta
     2161 ctcgctggtg acccagcagc cgctcgcggg caaggcccag ttcggcggcc agcggttcgg
     2221 cgaaatggaa gtgtgggcgc tggaggcgta cggcgcggcc cacgtgctgc aggaaatgct
     2281 cacggtgaag tcggatgacg tgaacggtcg cagcaaggtg tacgaggcga tcgtcaaggg
     2341 gcagaacttg cccgagcctg gtattccgga gtcgttcaac gtcctggtga aggaactgca
     2401 ggcactcgga ctcaaggtca cgctgggcac caccgaatcc ggcgacgagt aagagggata
     2461 caatgatcga ttttcgcagc ggacgcgagc ccaagagctc gagcttcgac tatatcagca
     2521 tccgcatcgc ctctccggag gagatccgcg gaccccggga ccccaaggag cgcgagcggc
     2581 tggagctgca gggccagcgc acctggtggt cgtggggtga ggtgaccaag ccggagacga
     2641 tcaactatcg ctcgttcaag cctgagaagg acggcttgtt ctgcgagcgg atcttcggcc
     2701 cggtcaagga ctgggaatgc cactgcggca agtacaagcg catccgctac cgcggcgtga
     2761 tctgcgatcg ctgcggcgtg gaagtcacgc tgtcgaaggt ccggcgcgag cgcatgggcc
     2821 acatcgagct gagcgtgccg gtggcgcaca tctggttctt caagacgctc ccgtcgccga
     2881 tggggaacct gatcaacatc acgctccgtg acctcgagcg ggtcatctac tactcgagct
     2941 acatcgtcat cgagccgggt gaccaggaag tcgaggccag ccagctcctc gacgaagagg
     3001 agtacctcac cctgcgacag aaggcgcggg acgagggcga caccggcttc cgggccgaca
     3061 tcggggctcc ggcggtgcgc gcgctgctcg agcggctgga tgtgaagaag ctggcggagg
     3121 agcttcgcgc cggcgtggcg acggagacca gtcagcaccg caagaagcag atgctcaagc
     3181 ggctcaagat cgtggatgcg ttcctctcct cgggtgactc gggcgatctg cccaacatcc
     3241 cggcctggat gatcctcgat gtggtaccgg tcattccgcc tgatctgcgg ccgctggtgc
     3301 cactcgacgg cgggcgcttt gccacgtccg atctgaatga tctgtatcgg cgggtgatca
     3361 atcggaacaa ccggctccag aagctgatcc agcacaaggc gccggaagtc atcctccgga
     3421 acgaaaagcg gatgctgcag gaggcggtgg acgcgctctt cgacaatggc cggcgctcca
     3481 aggccattcg cggtcgtggc aagcggccct tgaagtcgct gtcggacatg ctgaagggca
     3541 agcagggccg gttccgccag aacctgctgg gcaagcgcgt cgattactcc ggccgctcgg
     3601 tcatcgtggt gggtccggag ctgcggctgc accagtgcgg tctgcccaag gcaatggcgg
     3661 tcgagctgtt caagcccttc atcatccata agctggtgga gaagggaatc gccgagacgg
     3721 tcaagcgggc caagaagatc gtcgagcggg agagcccgga ggtctacgag atcctggaag
     3781 agatcattca ggatcatccg gtgctgctca atcgcgcgcc gacgctgcac cgtctgggca
     3841 tccaggcgtt cgagccggtg ctggtggaag ggaaggcgat ccggatccac ccgctggtat
     3901 gcgccgcttt caacgcggac ttcgacggcg accagatggc cgtgcacgtg ccgctctcgt
     3961 tcgaggcaca gctcgaggcc cggctgttga tgatctcgtc gaacaacatc ctcaagccgt
     4021 ccgacggccg gccggtggcg gagccgtcgc aggacatggt gctcggcagc tacttcctca
     4081 ccaaagaccc cgcggggttc gaggaggaac tgcggcgggc cggggcgggg aagaaggaag
     4141 agaccgagaa gcgtctctgg aaggacgcca agcatttcgg ctcgctggcg gagatcgaga
     4201 tggcgttgct ggcgggcgcg gttaagtacc acaccccgat ccacttctgg tacgtcccgc
     4261 cgcgggaaac cgtggacgag gagatgcggc gtccgcgctg ggtccggacc accgtggggc
     4321 gggtcctctt caacagcatc gtgcccaagg ccgtgatcgt ggagcgtggc ttccggaact
     4381 atgtgatgaa gaagaagcag ctctccgagc tggtgctcga ggcgtaccgc cgggcaggtc
     4441 tgtccgacac ggtcctgttc ctcgaccggc tcaaggagtt cggcttcggc ttcgccaccc
     4501 gcggcggcgt ctccatcggg atcgaggacc tcgagatccc gaccgagaag gccgatctcc
     4561 tgaacgatgc ggacggccgg gtgaagcggt tccagcgggc ctacaacacc ggtcagatca
     4621 cgttcggaga gcggtacaac aaggtgatcg acgcctggac ccacgccaac aacgacgtgg
     4681 ccgaggcgat ggtgaaccac atgcggcaga gccggggcgg gttcaacccg gtgtacatga
     4741 tgttcgactc cgggtcgcgc ggttcgcgtg accagatccg tcagctggcg ggcatgcgcg
     4801 gtctgatggc caagccgcag aagaagctca ccggcggcat cggcgagatc atcgagagtc
     4861 cgatcaagtc gaacttccgc gagggcctca ccgtgctcga gtacttcatc tcgacgcacg
     4921 gcgcgcggaa gggtcttgcc gatacggcgc tcaagacggc ggacgccggg tatctcaccc
     4981 gccgacttgt ggacgtggcg caggacgtca cgattaccga ggaggactgc gggacgattc
     5041 tcggactcga gatcgcggca ctcaaggagg gcgaagacat catcgagccg ctccgggagc
     5101 gcatcgtcgg cacggtggcg tcggaggacg tgttcgatcc gcacgagctg gacgagcacg
     5161 gcgacccgca gctgctggtg caggccggcc agctgatcga cgaggagata gccgacgaca
     5221 tcgacgactc cggtatcgag aaggtcaaga ttcgctcggt actgacctgc gaagctcgtc
     5281 ggggtatctg ccggatgtgc tacggtcgca acctggccac catggacatg gtggacatgg
     5341 gcgaggccgt gggcattctg gcggcccagt cgatcggcga gccgggtacg cagctcacgc
     5401 tacggacctt ccacatcggc ggcacgtcgt cacgcattgc ggaagaggcg gagcggcggg
     5461 cgcggtcgga cggcagggtg cagtacacgg aaggtctcga gtggaccgac ctgccggtgg
     5521 agtacgagga cggcaccaac gccaccatcc gggtggtgct cacgcgcgag gacgagtcgg
     5581 cggcggagga gcacaaggaa ggcatcctgg tggtggaccc gaaggacccg aagcgggttt
     5641 tgaaccggta tcctgtcccg gagggcgcac tcctcgccgt gagggaaggg gataccgtca
     5701 cgaagggcga caacgaccgc cgggcgacca tcctctatac ctgggatccg tacaacgatc
     5761 ccagcatcat caagtccgat ggcgagctgc gctggaagga cctggtgccc ggcgtgacac
     5821 tgcgggaaga gctcgacgaa ggaaccggtc tgcggtcgat cgtggtcatg gccgatccgg
     5881 atcgggagct gcatcccagc gtgctggtgt ataccagcca ccgcaaagac ccgcaggaat
     5941 acactctggc ggaaggcagc cgcatcattc tgggctcgcc caccgaccag gtgagccggg
     6001 ccatggagat tccggacgag gccaacccct ggagcaccaa gctccatgtc gacgagcgtc
     6061 cgatcaacga ggcgtggccc tcgaagagca agaaggagag caaggacaag accgtcttcc
     6121 tctcgacgcc ggtcaaggtg gcgaagggcg tgacggtcac caagatcccg cggcaggcgt
     6181 acaagacgcg cgacatcacg ggcggtctcc cgcgcgtggc ggagctgttc gaggcgcgcc
     6241 ggcccaagga tccggccacc atctccgagg tcgacggcgt ggtgaagttc ggcgagatca
     6301 agcgcggcaa gcgcgagatc tacgtctacc ccgacggcac cgacagcgag accccggcgc
     6361 agctctacga ggtgccggca ggcaagcacc tccgggtgca cgagggtgac cgggtgcggg
     6421 cgggcgaccg cctcaccgaa ggaccggtca acccgcacga catcctgctg atcaagggac
     6481 cgcgtgcggt gcaggagtat ctgcttaacg aggtgcagga ggtctaccgc ctgcagggcg
     6541 tacgtatcgc cgacaagcac atcggcgtga tcgtgcgcca gatgctccag aaggtccggg
     6601 tcaccgaccc gggtgatacc gacttcctcg agggagagac ggtggaccgg ttgagcttcc
     6661 gggacgagaa cgaccggacg ctgcggcgga agggtactcc agcgcaggcg gagccggtgc
     6721 tgctcggcat caccaaggcg agtctcacga cccagtcgtt catctcggcg gcgtcgttcc
     6781 aggagaccac gcgggtgctc accgatgcgg cgatccgtgg ggccaaggac gacctcctcg
     6841 gtctcaagga gaacatcatc atcgggcatc tgatcccggc gggcacgggc ctctcgcgct
     6901 atcgcaaggt ggaagtgatt ggagatgaag aagatgaggt cgagaaggcc tcgccggtgg
     6961 aggaagaggt aaaaacagct tgaccggacc ccggtcggct gggtaccatg ccctttcttt
     7021 gcgccgaaaa ccaattaatg gagacagtta ggtgccgact ctgaatcagc tcgtgcggca
     7081 tggccgcagg agacagctct cgaagaccgc gtcacccgct ctcaaggcgg cgccgcagaa
     7141 gcgcggcgtg tgcacgcgcg tctatacgtc gacgccgaag aagccgaact cggcgctccg
     7201 caaggtcgct cgagtccgtc tcacgaacgg gatggaagtg acgtgttaca tcccgggtga
     7261 ggggcacaat ctccaagaac actcgatcgt tctgatccgg ggaggccgcg tcaaggacct
     7321 ccccggcgtc cgttaccacg tggtccgcgg cacgctggac gcctcgggcg tggacggccg
     7381 ccagcagagc cgttccaagt acggcacgaa acggaagaag ggggcgtagg gaatgcgccg
     7441 cgccgcgaag atccatcgaa tcgagctcca gccggatgcc cggtacaaca acgtgctcgt
     7501 cacccgcttc atcaactgtc tgatgtatgc gggcaagaag agcacggccg agcgcatctt
     7561 ctacggggcc atggacgtga tcgagaagaa gacgggccag gacggcctga cggtcctcaa
     7621 ggccgccatg acgaacgtga agcccgtact ggaagtgaag tcccgccgcg tcggaggcgc
     7681 tacgtatcag gtgcctgtcg aggtgcggca ggaccgccgg acggcgctcg cgctccgctg
     7741 gctcattcag
//
LOCUS       DASRJQ010000171         3293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3544890, whole genome shotgun sequence.
ACCESSION   DASRJQ010000171 DASRJQ010000000
VERSION     DASRJQ010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3544890"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2836)
                     /locus_tag="VFB89_03870"
     CDS             complement(<1..2836)
                     /locus_tag="VFB89_03870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZM26467.1"
                     /translation="MSTSTFRQRRGSSHGTAYLCDAIAVIVIGTALAAMIGWTTGNRA
                     LVQVLPDYPPLYPGSALGLAAAAAGLWGLAHRIPRWVLTGAGIAALLGGLALVEYSAN
                     IDLGIDRLLFVKGVAAGGGHDRFAGRMVMSTAVSFLLTGTALVLGLAKAPWLRRAGSA
                     FTAAVVGAFALVTFVCYAGGVLDEFRFDVLAGMSIHVSLGLGLIAAGLVALAWEDETA
                     GSVSALPYWAPWAVGLASLTITLFVWRALVAQHQRQLIVETRSQAFVARHDLERATDG
                     LVTTLRVLPASALTDPDSAQAWPATLRRLLDEVPGMMSALRTDRSLNVHIPGKTSASD
                     SSLSRALRAALRARETPSGRLTRDSVLVGVAPTTDNQRLFTLAVPVCGGGECTGYIVA
                     LLNAQTFLANALHGATPGYHLVVSFRGEQLFRPAAITRTGLEVAQVVSVDLGSLHLDI
                     EVWPTEERLAVVHPELPDVVAALGLLLSGLLVLTFWLVRRSLLQTRLEQRARLSLALE
                     TATDGVWEWDVRANSGTVSDALWRRLGYSSAELALVNSLRQWRALIHPDDARRVQQAL
                     DRHVAGESASFEVEYRIKAQDGDWHNIVERGRVAERDIRGRPLVVLGIAADVTERKRA
                     DEALAANERRFRTMFDSAFQYQCLLDLDGACLEANHAVLEFAGTTMNTVRGRKLWETP
                     WWQGTTEEQRAHLRRACTEAVQGRTVRFEIEWAGAGGPALVDFSVKPILDGEGRVSQL
                     LAEGRDVTDRLRAENAMREMETLSSMGRLAARVAHEVNNPLAGIQNSFLLIKDAIPDS
                     HPYHSYVGAIEREIGRISAVTRQLYETYRPDPDGRRDSSVQTILSDAATLLGQVNRGS
                     KVNVEVDAVGVPAKLPVPGALLRQAAYNLVQNAIDASPPGGVVKVVAQADNSTFSLSV
                     CDQGPGVPAHLREQIFAPFFSTKRGGRTSGM"
     gene            complement(2977..>3293)
                     /locus_tag="VFB89_03875"
     CDS             complement(2977..>3293)
                     /locus_tag="VFB89_03875"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HZM26468.1"
                     /translation="GRRCRGMEGILAEAYEVLEPYSPGSVTDAALIAAAQRIEHYEIA
                     AYECVRRYALLLGRPDIADFVQQSLEEEEDANRRLAAVAEHQIDFRALADAGAEAIER
                     AS"
BASE COUNT          592 a         1151 c         1054 g          496 t
ORIGIN      
        1 ccatgccgct ggtgcggccc cctcgcttgg tgctgaagaa aggcgcaaag atctgctctc
       61 gcaaatgggc agggacgccc ggcccctgat cgcacaccga gagcgagaag gtggaattat
      121 ccgcctgggc cacgaccttc accacaccgc ccggtggtga ggcatcgatc gcgttctgaa
      181 ccaggttgta cgcagcctgt cgcagcaggg cgccgggtac gggcaacttg gccggcacac
      241 ccacggcatc cacctcgaca ttcaccttgg atccgcgatt cacctgtccc aggagtgtgg
      301 cggcgtcgga gaggatggtt tgcaccgacg agtcccggcg accatccgga tcgggacgat
      361 acgtctcgta caactgtcgc gtcacagccg aaattcgacc gatctcccgc tcgatcgcac
      421 cgacgtagga gtggtaggga tgggaatcgg gaatcgcgtc cttgatcagc aggaatgaat
      481 tctggattcc cgccagggga ttgttgactt cgtgcgccac gcgggccgcc agtcgcccca
      541 tcgagctgag tgtctccatc tcccgcatcg cattctcggc ccgcaagcga tcggtcacgt
      601 cgcgcccttc cgccagcagc tgcgagacgc gcccttctcc gtccaggatc ggcttcaccg
      661 aaaagtcgac cagcgccggc cctccggcgc cggcccactc gatctcgaaa cgcaccgtgc
      721 gcccctgaac tgcctccgta caggcgcgcc gcaaatgagc tcgttgctcc tccgtcgtgc
      781 cctgccacca gggtgtctcc cagagcttcc ggccccgaac cgtgttcatg gtggtcccgg
      841 cgaattcgag cacggcatga ttggcttcca gacaagcgcc gtcgagatcg agcaggcact
      901 gatactggaa agcgctatcg aacattgtcc ggaaacgtcg ctcgttcgca gccagggcct
      961 cgtccgctcg ctttcgctcg gtgacgtcgg ccgcgatccc cagcaccaca agcggccgac
     1021 cccggatgtc gcgctcggct acccggcccc gctcgacgat gttgtgccaa tcgccgtcct
     1081 gtgccttgat ccggtactcc acttcaaatg aggccgactc gccggccacg tgccgatcca
     1141 gagcctgttg gacccggcgc gcgtcgtccg gatgaatcaa tgcccgccac tgccgcagac
     1201 tgtttaccag cgccagctcc gccgaggaat agcccagccg acgccacagc gcatcgctca
     1261 cggtcccgct gttggcgcgt acatcccact cccagacccc atcggtcgcg gtctcgagcg
     1321 ccaggctcag acgggcgcgc tgctcgagcc gggtctgcag caggcttcgc cggaccagcc
     1381 agaacgtgag caccaggagc ccgctcagca gcagacccag ggcggcgact acgtcgggca
     1441 gctccggatg cacgacggcg agccgctcct cggtgggcca gacctcgata tcgaggtgaa
     1501 gcgaaccgag atcgaccgag acgacctgcg ccacctccag gccggtacgg gtgattgccg
     1561 cggggcggaa cagttgttca ccgcgaaacg acacgaccag gtggtacccc ggagtcgcgc
     1621 cgtgcagtgc attggccaga aacgtctgcg cgttgagcag tgcgacgatg tagccggtac
     1681 actcgccacc gccacacacc ggcacggcga gggtgaagag ccgctggttg tcggtggtag
     1741 gcgcgacacc gaccagcacg ctgtctcgcg tgaggcggcc gctcggtgtc tcacgcgctc
     1801 tgagcgcggc gcgaagcgcg cgggacaacg aggaatcgga cgccgaggtc ttgccgggga
     1861 tatggacgtt gagcgagcgg tcggtccgca gcgctgacat catcccgggc acctcatcca
     1921 gcagccgccg gagtgtcgcg ggccaggcct gcgcgctgtc ggggtccgtg agtgcgctgg
     1981 ccgggaggac gcggagtgtc gtgaccagcc cgtcggtggc tcgctcgaga tcgtggcgcg
     2041 ccacgaaggc ctgcgaacgg gtctcgacga tcagctgccg ctgatgctgc gccaccagcg
     2101 cgcgccagac gaacaacgtg atggtgagcg acgcgagccc gaccgcccac ggcgcccagt
     2161 acggcagcgc tgagaccgat cccgcagtct cgtcttccca cgccagcgct accagtcccg
     2221 ctgcaatcag gccgagaccc agcgagacgt gaatggacat cccggccagt acgtcgaacc
     2281 ggaactcgtc caacactccg cccgcatagc agacgaatgt gacgagcgcg aatgcgccta
     2341 cgaccgcagc ggtaaaggcc gaccctgccc gccggagcca gggcgccttc gcgagcccga
     2401 gcaccagcgc tgtgccggtc agaaggaagg acacggccgt ggacatgacc atgcggcccg
     2461 cgaaacggtc gtgcccgccg ccggccgcga cgcccttcac gaacaacagc cgatcgatgc
     2521 ccaggtcgat gttggcggaa tattcgacca gcgcgagtcc gccgagcagc gcggcaatgc
     2581 ccgctccggt cagcacccaa cgggggatcc ggtgggcgag accccagagc ccggcggccg
     2641 cggccgcgag accgagagcc gagccggggt agagcggcgg atagtccggc agcacctgca
     2701 ccaacgcccg gttgcccgtc gtccatccga tcatggcggc gagggccgtt ccgataacga
     2761 tcacggcgat ggcatcgcac aggtaagcgg ttccgtgcga gctgccgcgg cgctgtcgga
     2821 acgtgctggt cgacacgcta gtcattggga agaaactggt cctcaccctc acacggggga
     2881 caataatggg gcgaggtggc cctgaaaacg cgaactacgc cctcccgccg cggccggccg
     2941 ccggcggaag cagggtcaac gatatgtgaa gccggcctag ctggcccgct ctatcgcctc
     3001 ggcgcccgcg tcagccagcg cccggaaatc gatctggtgt tccgccacgg cggcgagccg
     3061 ccgattggcg tcttcctcct cttccagcga ctgctgcacg aaatcagcga tgtcgggccg
     3121 acccaggagc aacgcatagc gccgcacgca ctcgtaggcc gcgatctcgt agtgctcgat
     3181 ccgctgtgct gccgcaatca acgcagcatc ggtcacactg cccgggctgt agggttcgag
     3241 gacctcgtat gcttcggcca gaatgccttc cattccgcgg cagcgacggc ccc
//
LOCUS       DASRJQ010000172         3022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2417601, whole genome shotgun sequence.
ACCESSION   DASRJQ010000172 DASRJQ010000000
VERSION     DASRJQ010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2417601"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..321)
                     /locus_tag="VFB89_03880"
     CDS             complement(118..321)
                     /locus_tag="VFB89_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26469.1"
                     /translation="MYDESVLGGSAIGPHGVAMMTEIRGASNLKAKTVLGWKLRWPSW
                     RQRFRRGMDAPIPSVRYMEESRR"
     gene            580..1635
                     /locus_tag="VFB89_03885"
     CDS             580..1635
                     /locus_tag="VFB89_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26470.1"
                     /translation="MKRFIDWLRSGTPLAVFLKIVGTVSAVISLFLGAVQVKNVVGKW
                     RSSQAAAKELVAAADLKLGTGDYAQAWSLASQAVDSYPSFEPARAKQLEVAMAWLRDI
                     SVTGGTADPSFSAVSDKVTPVLLRAASRSEGKAKADVLAHLGWLTFLKWRDGAHNLSV
                     AQPYQQALAVDKDNLFAHVMLGHWLLYPAGGNGSLADARTHFDAALAAAKTNDERQFV
                     RSWQMAALLNRDETFAPELVRVSAAMAAENDTLSAKSRSRILWLVYTLPRWRGLDHLT
                     DSVTLAQQRAAFPWLTRGFDQPDPLYSAVSARLTELSGDSAEALERYKAILKQSPTLS
                     PAGREYLTHATQRLSRH"
     gene            1607..>3022
                     /locus_tag="VFB89_03890"
     CDS             1607..>3022
                     /locus_tag="VFB89_03890"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZM26471.1"
                     /translation="MPPNASVDTDVLVVGAGPTGLMLANQLMRRGVRTMIIDRHAGPA
                     RETRALGVQARTLEIYQHLGIVDRALELGKRTIAASMWAHGKRAARVPVGDIGKGLSP
                     YPFLLILGQDDNERLLGESLNQKALSVQWNTELMGLAQQSDRVVASVRNSDGTTRELT
                     ASWVGGCDGARSTVRENSGIAFQGAPYEHVFFVADTIVTGPMAQDELNVYLWRGGFHL
                     FFPMRGADHWRVVGILPPELRNRDDVKLDDVAPAIRTEAGAGLVFQKCTWFSTYRIHH
                     RRAERFRDRRCFLMGDAAHIHSPVGAQGMNTGLQDAYNLAWKLALVVAGRADAALLDS
                     FEVERIPVARRLLSSTDRAFSGIVSHGALAVFFRTRLFPGMLSLAMRIEKARKTAFRT
                     ISQIGIRYRGSPLSQTVAGLPEGAPRAGDRFPWLNLKLTPNGPGEDFYQKLDDTRFTL
                     VLTGQPAPRDAVAPGDLLRTHV"
BASE COUNT          521 a         1023 c          938 g          540 t
ORIGIN      
        1 tgatcggccg gtagcgaagg gggtccttcg ctaccagcac gaacaccatc tgccacagaa
       61 gcgcgagacc gatgaacccg tagaccgccg ccagggtaaa tacgactcgc gcgaacctca
      121 tctgcggctt tcctccatgt agcgcacgga aggaatgggc gcgtccattc cgcgccggaa
      181 ccgctgacgc caactgggcc atctgagttt ccatcccaag actgttttcg ccttcaggtt
      241 cgacgcgccg cggatctcgg tcatcatcgc cacgccgtgc ggaccgatcg cgctcccgcc
      301 tagaacactc tcatcgtaca tctccttcgc tcgccattcc ctctcctgct cgacgcggcc
      361 tcttgagacc gtcgcgcccg cttgccggca cttgtgcaac cggagaggag cgcggggaga
      421 tccgccgcaa ccggaagccc gccgatctgg acatgacgct ccagcacaag tggccggcat
      481 gcgagggaag caaaggagtc gagcagtcgt gcaatgctgg agactagctc ctgcctgcta
      541 gccgactcgc gcggccggcg acaaatttcc gccatccaca tgaagcgatt cattgactgg
      601 ctcaggtcag gcactccgct ggccgttttt ctcaagattg tcggcaccgt gagcgcggtg
      661 atctcgctct ttctaggcgc cgtgcaggtt aagaatgtgg tcggcaagtg gcgctcgagc
      721 caggcagcgg cgaaggagct ggtggcagcc gccgatctca agctcggcac cggtgactac
      781 gcgcaggcct ggtcgctggc ttcgcaagcg gtggactcct acccctcgtt cgagccggcg
      841 agagccaagc agctcgaggt cgcaatggcc tggctgcgcg atatatcggt caccggcggc
      901 acggccgacc ccagcttttc ggcggtctcc gacaaggtca caccggttct gcttcgggcg
      961 gccagccgca gtgagggaaa ggcaaaggcc gacgtgctgg cccatctcgg ctggctcacg
     1021 tttctcaagt ggcgagacgg cgcgcacaac ctctctgtcg cccagcccta tcaacaggcg
     1081 cttgccgtcg acaaggacaa cctcttcgcg cacgtgatgc tgggccactg gctgctctat
     1141 ccggccggcg gcaacggctc gctggcagac gcacgaacgc acttcgatgc cgcgctcgcg
     1201 gccgcgaaga cgaacgacga gcggcagttc gtccgctcct ggcagatggc ggcactcctc
     1261 aatcgcgacg agaccttcgc gcccgagctg gtccgggtct ccgccgcaat ggccgccgag
     1321 aacgacacgt tgagcgcgaa aagccgctca cgaattctgt ggctggtata tacgcttccg
     1381 cgctggcgcg gcctcgatca tctcaccgac agcgtgacgc tggcgcagca gcgggcggct
     1441 ttcccctggc tcacccgcgg cttcgaccaa cccgatccct tgtacagcgc cgtgtcggct
     1501 cggctcaccg agctgtcggg cgactctgct gaagcactcg agcgctacaa ggcaattctg
     1561 aagcagtcgc caactctatc acctgccggc cgggagtatc tcacccatgc cacccaacgc
     1621 ctcagtagac actgacgtcc tcgtcgttgg agccggaccc accgggttga tgctggccaa
     1681 tcagctgatg cggcgcggcg tacgtaccat gatcatcgac cggcatgccg gccccgcgcg
     1741 cgagacccgg gccctcggcg ttcaggctcg gactctcgag atctatcagc atcttggcat
     1801 tgtcgatcgg gcactcgagc tgggcaagcg taccattgcc gcgagcatgt gggcccacgg
     1861 caagcgggcc gcgcgagtac cggtgggcga catcggaaag ggcttgagcc cgtatccctt
     1921 tctgctgatc ctggggcagg acgacaacga gcgcttgctg ggggagtcac tcaatcagaa
     1981 agcgctctcg gtacagtgga acaccgagct gatggggctg gcgcagcagt cggatcgtgt
     2041 ggtcgcctcg gtgagaaact cggacggtac gacacgggag ctgaccgcat cctgggtcgg
     2101 cggctgcgac ggcgcccgca gcacggttcg ggagaacagc ggcattgcct ttcagggcgc
     2161 gccctacgag cacgtcttct tcgtcgccga taccatcgtc accgggccga tggcccagga
     2221 cgagctcaac gtctatctct ggcgcggcgg gttccatctt ttctttccca tgcgcggcgc
     2281 agatcactgg cgggtggtcg gcatcctgcc gcccgagctg cgcaaccgcg acgatgtgaa
     2341 actcgacgat gtggcacccg caatccgcac ggaggcaggt gccggcctgg tctttcagaa
     2401 atgcacctgg ttttcgacct atcggatcca tcaccggcgg gccgagcgct ttcgcgaccg
     2461 gcgttgcttc ctgatgggag atgcggccca cattcacagt cccgtgggcg cgcaggggat
     2521 gaacacggga ctccaggacg cctacaatct ggcctggaag ctcgcgctcg tagtcgccgg
     2581 tcgggccgac gcggcgctgc tcgattcctt tgaggtcgag cggattccgg tcgcgcgccg
     2641 attgctgagc agcaccgatc gggcgttctc cggcatcgtc tcccatggcg ctctggccgt
     2701 gttcttccgc acccggctct tcccgggaat gctttccctc gcaatgcgga tcgaaaaggc
     2761 tcgcaagaca gcgtttcgca ccatttcgca gatcgggatc cgctaccgcg ggagtccgct
     2821 gtcccagaca gtggcgggcc tccctgaagg cgcgccgcgc gcgggggatc gcttcccctg
     2881 gctcaacctc aagctgactc ccaacggccc cggcgaggac ttctatcaga agttggacga
     2941 cacccgcttc acactcgtcc tcaccggcca gccggcgccg cgcgatgctg tcgccccggg
     3001 tgatctcctg cgcactcatg tg
//
LOCUS       DASRJQ010000173         2914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3263484, whole genome shotgun sequence.
ACCESSION   DASRJQ010000173 DASRJQ010000000
VERSION     DASRJQ010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3263484"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /gene="tilS"
                     /locus_tag="VFB89_03895"
     CDS             complement(<1..912)
                     /gene="tilS"
                     /locus_tag="VFB89_03895"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HZM26472.1"
                     /translation="MTRTESLRSAFAAELDQLQLGRGRLLVAVSGGRDSVALLDLLAA
                     ASVDRGLILTVGHVDHGIHPASAVVAAAVRRLAEEYAVPYLERRLSLGADAGETTARA
                     GRYRALEAMRRAASADFIVTAHHADDQAETVLMRALHGSGPAGLAGMAPRRGRVLRPL
                     LPFRRGDLARHLLQIGRAGWDDPANTDPSHFRSWVRCRVMPELVERLPDVAARLNAVA
                     RQARVDRAAWDSLLDMLPGLAFKVEPDGVSVGLEVLEAADDNLLLCLLMAICRRTEFS
                     VGPTRLGRVSELIRLAKSGARVPIGEGA"
     gene            complement(949..1362)
                     /gene="apaG"
                     /locus_tag="VFB89_03900"
     CDS             complement(949..1362)
                     /gene="apaG"
                     /locus_tag="VFB89_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HZM26473.1"
                     /translation="MSGLRPPPRFFYRLTNGIRITVRPEYLAARSDPTQRRFVFAYHV
                     RIENVDNRSARLISRKWLIHDEIGEDVEVEGEGVVGEQPVIGPGEVHEYQSFIVLKAP
                     SGYMEGSYRFIRADGSAFEAEIPRFALIVAELGAG"
     gene            complement(1377..1655)
                     /locus_tag="VFB89_03905"
     CDS             complement(1377..1655)
                     /locus_tag="VFB89_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26474.1"
                     /translation="MSRAFVNEDAGGEAPRYLLPHPDDPAYDAAAARALLRGADEGDS
                     ASAEAATGYVFGEPRLRPHVERILEEARESGDERLEQLAERFLRRAAR"
     gene            complement(1659..1865)
                     /locus_tag="VFB89_03910"
     CDS             complement(1659..1865)
                     /locus_tag="VFB89_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26475.1"
                     /translation="MHGADAEKVELIARLVAVMGRTFSTAEGREAGVRTLSDRLLRLD
                     DATLRQLAASFGLDEPGEPEPEEG"
     gene            complement(1892..2521)
                     /locus_tag="VFB89_03915"
     CDS             complement(1892..2521)
                     /locus_tag="VFB89_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HZM26476.1"
                     /translation="MSFMDRADAGRRLAAALTPLRQRDPVVLALPRGGVPIGYHVARA
                     LGAPLDVIVVRKLGAPDHEELAVGAIAREAVYIDWLIVRQLGVTQAYLEQVIAAEKEE
                     LERRERAYRGGRPALPVKGRTVIVVDDGLATGATFRAALAAVRIEQPASIVVAVPVGA
                     PESVARLRGVADDVVCLEMPPDFRAVSLSYEDFSPTSDAEVVECLEAAR"
     gene            complement(2560..>2914)
                     /locus_tag="VFB89_03920"
     CDS             complement(2560..>2914)
                     /locus_tag="VFB89_03920"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZM26477.1"
                     /translation="TLSQRPQRMGTDNTRSHVRIHYPSTRRPRLVLPGGAHEVLDLSA
                     EGLLVTVPESAAKPTLGTRVEGTLELATAGPLAIRARVVWVDGDRVGLHLWPDAIPYP
                     VLLAEERNVAPTRSN"
BASE COUNT          505 a         1009 c          919 g          481 t
ORIGIN      
        1 ggcgccttct ccgatgggaa cccgggctcc gctcttcgcc aatctgatca gttcgcttac
       61 cctgccgagg cgggttggac cgaccgagaa ttcagttcgc ctgcagatgg ccatcagcag
      121 gcacaacaac aggttgtcat cagcggcttc gagtacttcg aggcctacgg agaccccatc
      181 aggttcaacc ttgaaggcga gccccggcag catatcgagg agcgagtccc acgccgcgcg
      241 atctaccctg gcctgtcggg ccactgcgtt cagtctggcg gcgacgtcgg gcagtcgctc
      301 cacgagctcg ggcatcaccc ggcaccgaac ccaggaccgg aagtgactcg ggtcagtatt
      361 ggccggatcg tcccatccgg ctcgtccaat ctggagcaga tgccgggcca agtcgccccg
      421 tcgaaaaggc agtagcgggc ggagcacgcg gcctcgacgt ggggccatcc cagcgagccc
      481 ggcaggtcct gacccgtgca gagcccgcat gagcaccgtc tcggcctggt cgtccgcgtg
      541 gtgtgcggtc acgatgaagt ctgccgatgc cgcgcggcgc atcgcttcca gcgcccggta
      601 ccgcccagca cgcgcggtcg tctcgcctgc gtcggctccc agcgagagcc ggcgctcgag
      661 gtacggcacc gcgtactcct cggctaatcg gcgtaccgct gcggcgacga cagcgctcgc
      721 cgggtgaatt ccatggtcaa cgtgaccgac ggttagaatc agccctcgat cgacggacgc
      781 agcggcgagc aggtcgagca gggcgactga gtctcgcccg ccggacactg ccacgagcaa
      841 gcgtccccga ccgagctgca gttggtcgag ctcggcggcg aatgcagagc ggagagattc
      901 ggttcgtgtc acagccggaa aggtaacaat tcggccgtgg gtaatcgctc agccggctcc
      961 cagctccgcc actataagcg caaaacgcgg gatctccgcc tcgaatgcgc tcccgtcggc
     1021 gcggatgaag cggtacgacc cctccatgta gccgctgggc gctttgagca cgatgaagct
     1081 ctgatactcg tggacctcgc ctgggccgat gaccggctgc tcgcccacga ccccttcccc
     1141 ttcgacctcc acatcttcgc cgatctcgtc gtgaatcagc catttccggc tgatcagccg
     1201 ggcactccga ttgtcgacgt tctcgatccg gacgtgatag gcgaagacga accgacgctg
     1261 cgtcggatcg gaccgggcgg cgagatattc cggccggact gtgatccgga tgccgttcgt
     1321 caggcgatag aagaaccgcg gcgggggccg aagtccgctc atgtgctcct cagcgatcac
     1381 cgcgcggcgc gccggagaaa acgctcggcc agctgttcca gccgctcatc cccggactcc
     1441 cgcgcttcct cgagaatacg ttcgacatgg gggcgcagtc ggggctcacc gaagacatag
     1501 ccggttgctg cctccgcgct cgcggaatcg ccctcgtccg ccccccgcag cagggctcgc
     1561 gccgccgcgg catcgtaggc cggatcatcg ggatgcggaa ggagataccg cggggcctct
     1621 cccccggcgt cttcattgac gaatgcgcgg gacatggttc acccctcttc cggctccggt
     1681 tcgccgggct cgtccagccc gaagctcgcg gcgagctgtc gcagcgtcgc gtcgtccagt
     1741 cgcagcaggc gatcgctcag ggtccgcacg ccggcttcgc ggccttcggc agtcgagaag
     1801 gtccgcccca tcacggcaac gagacgagcg atgagctcga ccttttcggc atcggctccg
     1861 tgcatcgaca ctccggaaag cgagggatga ctcaacgagc tgcttcgagg cattctacca
     1921 cctcggcatc gctcgttggg gaaaaatcct cgtaggaaag actcacggct cgaaagtccg
     1981 gcggcatctc gaggcacacc acatcgtcgg ccacccctcg aaggcgagcc acgctctcgg
     2041 gtgctccgac cggtaccgct acaacaatgc ttgccggctg ttcaattcgt accgcagcga
     2101 gcgcggcgcg aaacgtggcg ccggtggcca agccgtcgtc cactacgatt acggtcctgc
     2161 cttttaccgg cagtgccggt cggccgcctc gataagcccg ctcgcgccgt tccagctcct
     2221 ccttttcggc cgcaattacc tgctcgaggt aggcttgcgt cacgcctaac tggcgcacga
     2281 tcagccagtc gatgtagacg gcctcccgcg cgattgcgcc gacggccagc tcctcgtggt
     2341 ccggcgctcc cagctttcgg accacgatca cgtccagcgg cgcaccgagc gcccgcgcga
     2401 cgtgataccc aatgggaact ccgccccgag gcaatgcgag gactacggga tcgcgttgcc
     2461 gaaggggagt cagcgccgcc gcaaggcgac gacccgcatc cgcgcgatcc ataaagctca
     2521 tcgaggtatc cccgcacgac ctcggcagac agaccgcgat caattgctcc gcgtgggggc
     2581 gacgttacgc tcttcggcca gtaggacggg ataggggatg gcatccggcc agagatgcag
     2641 tcccacgcgg tcaccatcga cccagacaac ccgagcccgg attgccaacg gtccggccgt
     2701 cgccagctcc agtgtgcctt ccacccgggt acccagcgtc ggcttggcgg cgctttcggg
     2761 cacggtgacc agcaaccctt cggcagaaag gtccagcacc tcgtgcgcgc cgccaggcaa
     2821 cacgagacgc ggccgtcggg tggacggata gtgaatgcgg acgtgtgagc gtgtgttgtc
     2881 ggttcccatg cgttgcggcc tttggctcag agtg
//
LOCUS       DASRJQ010000174         7384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3586933, whole genome shotgun sequence.
ACCESSION   DASRJQ010000174 DASRJQ010000000
VERSION     DASRJQ010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7384
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3586933"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VFB89_03925"
     CDS             complement(<1..718)
                     /locus_tag="VFB89_03925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HZM26478.1"
                     /translation="MNVAPQFRKGDRVRLHPSARSDVFDLALTGRTATVSSVERDIEG
                     RFYVAVVVDDDPGISVGPRRPGHRFFFAPEEVDLAPAEEQPIGSETRTILVAGIGNIF
                     LGDDGFGVEVIRRLVDRPIPDGVQVADYGIRGFDLAYALANDPDHTILVDACPRGEEP
                     GSVSLIEPALDRLDEDESHPLVFDAHTMNPLSVLRLARSMGAPLRHVLLVGCEPATLG
                     PDEGQLGLSPAVEAAVDQAVT"
     gene            complement(715..1656)
                     /locus_tag="VFB89_03930"
     CDS             complement(715..1656)
                     /locus_tag="VFB89_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26479.1"
                     /translation="MSDTIERIADALLYEGYLLYPYRRSAVKNRQRFNFGVLYPEEHV
                     AAGGSDTADRCFMQIECLVLGDAATTLSLKLRFLQLMESTAGADASAPWQEATERAVT
                     LRCSLAGLLTAPQRHEFSFPGGEEPTGRATHSLDGEVEFSARTIDDSALRLRVRVTNR
                     TQPTAGGAERSDVLARSLVSCHAILTLQAGKFVSLLDPPPELAAPAGSCENIGAWPVL
                     AGEAGDRNTLLASPIILYDYPTVAPESAGDLFDATEIDEILSLRILTLTDEEKREVRE
                     SDERARRLLERTEALTAEQLMQMHGVLRQPRLFEEHP"
     gene            complement(1653..2300)
                     /locus_tag="VFB89_03935"
     CDS             complement(1653..2300)
                     /locus_tag="VFB89_03935"
                     /inference="COORDINATES: protein motif:HMM:NF039986.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6084 family protein"
                     /protein_id="HZM26480.1"
                     /translation="MPDLSFQVDGAKVVPFALAPQLAFGLRVTNASTTEAVHTVVLRC
                     QLQVDVTRRRYAPEEEKRLRDLFGDPDRWSQTLRTMLWTHANTVVPAFVGETTAELLV
                     PCTFDFNVAATKYFYGLTSGDVPLTLLFSGSVFYQNAAGTLQVAPISWDKEATYRLPV
                     QLWQDLMDAYYPNEAWLHLRRDVFDRLYRYKVSRGIPSWEQALDGILPVEEEAPV"
     gene            complement(2293..2949)
                     /locus_tag="VFB89_03940"
     CDS             complement(2293..2949)
                     /locus_tag="VFB89_03940"
                     /inference="COORDINATES: protein motif:HMM:NF040271.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5947 family protein"
                     /protein_id="HZM26481.1"
                     /translation="MPISDPLDGARPFARLRELARRPTPAEHCDVCGQPVAENHDHLM
                     EPAARRLLCACGACAVLFSGQTGARYKRVPRDVRMLEQFELSDAQWDSLRLPINLAFF
                     FYSSPKNRVVACYPSPAGATESLLPLEAWEEVAEANPVLAGMEPDVQALLVNRVGYAR
                     GLAPAEYFIAPADRCYRLVGIIRTRWKGLSGGTEVWRDIAGFFSDLRTEAGRGGGSSA
                     "
     gene            complement(2952..3866)
                     /locus_tag="VFB89_03945"
     CDS             complement(2952..3866)
                     /locus_tag="VFB89_03945"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HZM26482.1"
                     /translation="MRLEGRIEQIEDLVLKLETGPEAQLRGIARELVQSLMEFHGAGF
                     ERVLEIVRRRGDADATMDHLAQDELVRSLLLLYGLHPVDVESRVREALDKTRPYLRSH
                     GGNVELVSVEADGAVRLRMQGSCHGCPSSAVTLKLAIEQAIHEAAPDVTSIVLVEDEA
                     RERHQPAQLSLVTIEPALRSNGNGQVDGRWETVPGLEELKQDEPRAIQVSGRSVVFCR
                     LGDTFFAYADRCPACGADLADGSLARGALACSACGAAYDVVRAGRGLDRPDLHLEPFP
                     LLVENGRARVALTLQHAGTAVGAGAETT"
     gene            complement(3863..5656)
                     /locus_tag="VFB89_03950"
     CDS             complement(3863..5656)
                     /locus_tag="VFB89_03950"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HZM26483.1"
                     /translation="MTTTAPVERAGTAGKSRKLVEMNWDPITRIVGSLGIFTKIDFEN
                     KEVAECHSTSSIFRGYSIFMKGKDPRDAHFITSRICGICGDNHATCAMYAQNMAFGVR
                     PPSLGEWIVNLGEAAEYMFDHNIFQDNLVGVDFCEQMVKATNPGVWAKAQKTEAPNAA
                     LHGYRTIADIMTALNPFTGSFYRETLMVSRYTREMFCLMEGRHVHPSTLYPGGVGTVP
                     TVQLFTDYLVRLARYAEFMKKCLPLHEDLFNFWYDALPGYEKVGYRRTLLGCWGSFNN
                     PDVCDYTYKNMSNWGDAMYVTPGVVVDGKLVTHDLVKINLGIRILLGHSFYDDWDNSE
                     TFVRKDPLGNPVDQRHPWNQTTLPRPQKRDFDGKYSWTMSPRWFDGKDHLALDTGGGP
                     IARLWATALAGIVDIGYVKATGSSVKMYLPRTATLPEVEFEWKIPKWSNAIERDRART
                     YFQVYAAACAYYFAEKALAELHAGRTKTWSEFTVPDQAIACGFHEAVRGVLSHHVVIR
                     NGKIANYHPYPPTPWNGSVRDVYGTPGPYEDSVQNTPIFEENGPENFKGIDIMRAVRS
                     FDPCLPCGVHMYLGNGKVIEQHHSPFFGGQP"
     gene            complement(5676..6737)
                     /locus_tag="VFB89_03955"
     CDS             complement(5676..6737)
                     /locus_tag="VFB89_03955"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26484.1"
                     /translation="MPATAPYGRKTQRAPAVPEIHIVWMTAGLGCDGDSVSITAATQP
                     SIEDVVLGAIPGLPKVHVHNPVLAFEVGDDFMKYWYQAEEGKLDPFVLVVEGSIPNEK
                     LSGDGYWAAMGTNLKTGQPIRTTEWIDRLAPKALAVIAIGTCATYGGIHAMSGNPTGC
                     MGLADYLGWEWRSKAGVPIVNVPGCPVQPDNFMETVLYLLYQVAGLAPMIPLDDQGRP
                     EWLFGSTVHDGCDRAGYYEQGDFATEYGSPKCIVKLGCWGPVVNCNVPKRGWMAGIGG
                     CPNVGGICIGCTMPGFPDKFMPFMDEPPGARISATSITVYGKAIRALRSITNNTLNKE
                     PKWRHSRAELTTGYQPRSY"
     gene            complement(6875..>7384)
                     /locus_tag="VFB89_03960"
     CDS             complement(6875..>7384)
                     /locus_tag="VFB89_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZM26485.1"
                     /translation="PDIVVLDISLPGRDGLDLLKSIRGTDPDLPILVLSMHDEAVYAE
                     RALRAGANGYMMKQEATDKVLTVLRRILRGEPYVSDRITTNLLQRIVTGPSKPKRASI
                     ASLSDRELEVFHLIGAGYGTREIAEELGLSVKTVESYQAHIKEKLSLKSARALVQRAI
                     EWRLSGEAI"
BASE COUNT         1288 a         2355 c         2373 g         1368 t
ORIGIN      
        1 tggtgacggc ctggtcgact gcggcttcga ccgcgggact caggcccagt tggccttcat
       61 ccgggccgag ggtcgccggc tcgcatccga ctagcaagac gtggcgaagg ggagcaccca
      121 tggaacgagc aagccgcagc acactcagcg gattcatcgt atgggcgtcg aagacgaggg
      181 gatgcgactc gtcttcgtcc agcctatcga gagcgggctc gatgagagag acacttcctg
      241 gctcctcacc ccggggacag gcgtctacca ggatggtgtg gtcgggatcg ttggcgagtg
      301 cgtatgcgag atcgaacccc cggatgccgt agtcggccac ctgaacgccg tccggaatgg
      361 gacggtctac gagccgccgg atgacttcca cgccgaagcc gtcgtcgcca agaaagatgt
      421 tcccgatgcc ggccaccagg atggtgcgcg tctccgatcc gatcggctgt tcctctgcgg
      481 gggcgagatc gacttcctcg ggggcgaaga agaagcggtg gccgggtcgg cgtggcccga
      541 ccgagatgcc tggatcgtcg tccaccacca cggccacgta gaagcgcccc tcgatatccc
      601 gttccaccga cgagaccgtt gcggtccggc cggtgagcgc gaggtcgaag acgtcgcttc
      661 gggcgctggg atggagccgt acccggtcgc ccttccggaa ctggggtgcg acgttcacgg
      721 gtgctcctcg aacagccggg gctgccgcag gacgccgtgc atctgcatca actgctcggc
      781 ggtcaatgct tcggtccgct cgagcaggcg tcgcgcgcgc tcgtcggact cccggacctc
      841 gcgcttctcc tcgtcggtca gcgtcagaat ccggagtgag aggatctcat cgatttcggt
      901 ggcgtcgaac agatcgcctg cgctctcagg ggcgacggtc ggatagtcgt acaagatgat
      961 gggcgacgcc aggagcgtat tccggtcgcc tgcctctccg gccaggaccg gccatgcgcc
     1021 gatgttctcg cagctcccgg ccggggcggc aagctcgggg ggcgggtcga gcaaggaaac
     1081 gaacttgcca gcctgcagtg tgaggatcgc atggcatgag accagtgacc gggccaggac
     1141 gtccgaccgc tcggcgccac cggccgttgg ctgagtacgg ttcgttacgc ggacacgaag
     1201 ccggagtgcg ctgtcgtcga tcgtccgagc ggaaaactct acctcgccat cgagcgagtg
     1261 cgttgcgcgc ccagtcggct cttctccgcc cgggaatgag aattcgtggc gctgcggcgc
     1321 tgtgagaagc cctgccaggg agcaacgcag ggttaccgca cgttcggttg cctcctgcca
     1381 gggcgccgag gcatccgctc cggccgtgct ctccatcagc tgaagaaacc gaagcttgag
     1441 cgacagcgtg gtcgcggcgt cgccaagcac caggcattcg atctgcatga agcagcggtc
     1501 cgccgtgtcg gaaccgccgg cggcaacgtg ctcttccgga tacagcacgc caaagttgaa
     1561 ccgctggcga ttcttgactg cggagcggcg gtatgggtac agcagatacc cttcgtagag
     1621 cagagcgtcg gcgatgcgtt cgatcgtatc gctcataccg gcgcctcttc ctccacgggc
     1681 aggatgccgt cgagggcttg ctcccagcta gggatgccgc ggctcacctt gtagcggtag
     1741 aggcgatcga agacgtcgcg ccgcaagtgc agccacgcct cgttggggta gtacgcatcc
     1801 atcagatcct gccagagctg caccgggagc cgataggtcg cttccttgtc ccaggagatc
     1861 ggcgctacct ggagagtgcc cgccgcgttc tggtagaaca cgctgccgct gaacaggagc
     1921 gtgagcggca cgtcaccgga ggtgagtcca tagaagtact tggtcgcggc aacattgaag
     1981 tcgaacgtgc agggaaccag gagttccgcc gtggtctcgc cgacgaacgc gggcacgacc
     2041 gtgttggcgt gcgtccacaa catggttcgg agagtctggc tccagcgatc ggggtcgccg
     2101 aagagatcgc gcaggcgctt ttcctcctcg ggcgcgtagc gccgccgagt gacgtcgacc
     2161 tggagctggc agcggagcac taccgtgtgc accgcttcgg ttgtggatgc gttggtcacc
     2221 cggagcccga aggccagttg cggcgccagg gcgaagggca ccaccttggc gccatccacc
     2281 tggaacgaca gatcaggcac tcgaccctcc cccgcgtccg gcttcggttc tcaagtcgct
     2341 gaagaagcct gcgatgtcgc gccagacctc ggtgcccccg gagagcccct tccaccgagt
     2401 ccggatgatc cccaccaggc gataacagcg atctgccggg gcaatgaagt actccgccgg
     2461 agcgaggccg cgagcataac ccacccgatt gaccaggagc gcttggacat ccggttccat
     2521 accggcgagc accggattgg cctcggcgac ttcctcccac gcttcgagcg gcaggagcga
     2581 ctcggttgcg ccggccgggc tcgggtagca cgcgaccacc ctgttcttcg ggctgctgta
     2641 gaagaagaac gcgagattga tcggcagcct gaggctgtcc cactgcgcgt cgctcagctc
     2701 gaactgctcg agcatccgga cgtcgcgcgg cacccgcttg tatcgtgcgc cggtctggcc
     2761 gctgaagaga accgcacacg caccgcacgc gcacagcagc cggcgcgcgg cgggctccat
     2821 gagatgatcg tggttctcgg cgaccggctg accgcaaaca tcgcagtgct cggccggggt
     2881 cggccggcgc gcaagctcgc ggagacgagc gaacggccgg gctccgtcga gcggatcgga
     2941 gatgggcatg cctacgtcgt ctccgcgcct gctcctacgg ctgtaccggc atgctgcagc
     3001 gtcagcgcga ctcgcgcccg cccgttctcg accagcaacg ggaagggttc gagatgcaag
     3061 tcgggccggt ccaatccgcg accagcccga accacgtcgt acgctgcgcc gcaggccgaa
     3121 cacgcgagtg ccccacgagc aaggcttcca tcggcgagat cggcgccgca tgccggacaa
     3181 cggtcggcgt aggcaaagaa agtgtcaccc aggcgacaga acactaccga tcggccgctt
     3241 acctgaatgg ctcgtggctc gtcttgcttc aactcttcga gccccggaac cgtttcccac
     3301 cgcccatcaa cttgcccgtt tccattactc cgcagcgcgg gctcgatcgt tacaagcgac
     3361 agttgcgcgg gctgatgccg ctcgcgagcc tcgtcttcta ccagcacaat ggacgttaca
     3421 tccggggccg cctcatggat ggcctgctcg atcgcgagct tgagcgtcac ggcagacgac
     3481 ggacatccgt gacagctccc ctgcatccgc agccgcaccg cgccgtcggc ttcgacactc
     3541 accagctcga cattgcctcc atgcgagcgg agataggggc gggtcttgtc cagcgcctcc
     3601 cgcactcggc tctccacgtc gaccggatgg agtccgtaca acagcaggag gcttcggacc
     3661 agttcgtcct gggcaaggtg gtccatcgtg gcgtcggcat cgccgcgccg gcgaacgatc
     3721 tccaggactc gctcgaaacc ggcgccatga aactccatga gcgattgcac cagctctctc
     3781 gctatgcccc ggagctgtgc ctccggcccg gtctcgagct tgaggaccag gtcttctatc
     3841 tgttcgatcc gcccttcgag cctcacggct gcccgccgaa gaagggcgag tggtgctgct
     3901 cgatgacctt gccgttgccc aaatacatgt gcacgccaca ggggaggcag ggatcgaagc
     3961 tgcgcaccgc acgcatgatg tcgatgcctt tgaaattctc cggaccgttt tcctcgaaga
     4021 tgggcgtgtt ctgcaccgag tcttcgtacg gtccaggcgt cccgtagacg tcgcgcacgc
     4081 ttccgttcca gggcgtgggc gggtagggat ggtagttcgc gatcttgccg ttgcggatca
     4141 cgacgtggtg cgagaggact ccgcgcaccg cttcatgaaa tccgcacgcg atggcctggt
     4201 cgggcaccgt gaactcgctc caggtcttgg tccttcccgc atgcagctcg gccagcgcct
     4261 tctcggcgaa atagtaggcg cacgccgccg cgtacacctg gaagtaggta cgggcacgat
     4321 cccgctcaat ggcgttactc cacttgggga tcttccactc gaattccact tccggcaggg
     4381 tcgccgtgcg aggcagatac atcttcacgc tcgagccggt cgccttgaca tagccgatgt
     4441 ccacgatgcc ggcgagcgcg gtcgcccaga gccgcgcgat tgggccgcca ccggtgtcga
     4501 gcgccagatg atccttcccg tcgaaccagc gcggcgacat ggtccagctg tatttgccgt
     4561 cgaaatcgcg cttctggggc cggggcaggg tggtctggtt ccagggatgc cgctggtcca
     4621 cgggattgcc gagcggatcc ttgcggacaa acgtctccga gttgtcccag tcgtcataga
     4681 acgaatggcc cagcaggatc cggatgccca ggttgatctt taccagatcg tgcgtgacca
     4741 gcttgccgtc taccaccacg cctggggtga cgtacatggc gtcgccccag tttgacatgt
     4801 tcttgtaggt gtagtcgcag acgtccgggt tgttgaacga tccccagcag ccgagcagcg
     4861 tgcgccggta gccgaccttc tcgtatcccg gcagggcgtc gtaccagaag ttgaaaaggt
     4921 cttcgtgcag cggcaggcac ttcttcatga actcggcata gcgggcgagt cgcacgaggt
     4981 agtcggtaaa gagctgaacg gtcggaacgg tgcccacgcc gcccggataa agcgtggagg
     5041 ggtggacgtg gcgcccttcc atcaagcaga acatctcccg ggtgtaccgg ctcaccatga
     5101 gggtctcacg atagaacgag ccggtgaacg ggttgagcgc cgtcatgatg tcggcgatgg
     5161 tgcggtagcc gtgcagcgcc gcgttgggag cttccgtctt ctgcgctttg gcccagacgc
     5221 ccggattggt ggccttcacc atctgctcgc agaagtccac gccgaccaga ttgtcctgga
     5281 agatgttgtg gtcgaacatg tactccgcgg cttcgcccag attcacgatc cattcgccga
     5341 gactgggcgg ccggacgccg aaggccatgt tctgggcata catcgcgcag gtggcgtggt
     5401 tgtcgccgca gatgccgcag atccggctgg tgatgaagtg ggcgtcgcgc ggatccttgc
     5461 ctttcatgaa gatgctgtag ccgcggaaga tcgacgacgt gctatgacac tcggcgacct
     5521 ctttgttttc gaagtcgatc ttggtgaaga tgcccaggct cccgacgatc cgcgtgatcg
     5581 ggtcccaatt catctccacc agcttgcgcg acttgccggc agtgccggct cgctcgacgg
     5641 gtgcagtggt ggtcatcgcg gtcgctcctt tggactcagt aggacctggg ctgatacccg
     5701 gtcgtcaatt cggctcggga atgccgccac ttcggttcct tgttcagcgt gttattggtg
     5761 atggaccgga gggcacggat cgctttgccg tagaccgtga tgctggtagc ggagatcctg
     5821 gcgcccggcg gctcgtccat gaagggcatg aacttgtcgg ggaaccccgg catggtgcag
     5881 ccgatacaga tgccgccgac gttggggcaa ccaccaatgc cggccatcca gccccgcttc
     5941 ggcacgttgc agttcaccac cggcccccag catcccagct tgacgatgca cttgggcgag
     6001 ccgtactcgg tcgcgaaatc tccctgttcg taatagcctg cccggtcaca gccatcatgg
     6061 acggtgctgc cgaacagcca ctccggtcgc ccctggtcat ccagagggat catgggcgcg
     6121 aggccggcga cttgataaag gagataaagg acggtttcca tgaagttgtc gggctggacc
     6181 ggacaccccg ggacgttcac gatggggacc ccggccttcg acctccattc ccatccgagg
     6241 tagtcggcga gtcccataca gcccgtgggg ttgcccgaca tcgcgtgaat gccgccgtag
     6301 gtggcgcaag tgccgatggc aatgacggcg agcgccttgg gggccaaacg gtcgatccac
     6361 tcggtggtcc tgatgggctg gccggttttg aggttcgttc ccatcgccgc ccagtagcca
     6421 tccccgctca gcttttcgtt ggggatcgaa ccttcgacca cgagcacgaa ggggtccagc
     6481 tttccttctt ctgcctgata ccagtatttc atgaagtcgt cgcccacctc gaatgcgagc
     6541 accggattgt gcacgtggac cttgggtaat ccaggaatcg cgccgagcac gacgtcttcg
     6601 atgctgggct gagttgccgc ggtgatcgag accgaatcac catcgcatcc aagtccagcc
     6661 gtcatccaga caatgtgaat ctccggaacc gccggtgcac gctgagtctt ccggccatag
     6721 ggcgctgtgg caggcatacg ctctccttcg gaagggtccg tccgctaagg ctatgactta
     6781 ggaattgttg agaaatgccg caatcgggcg ggccctacag gattaccgga gaactctggt
     6841 gcggcttacc gggatttccc cgacgtacga acggtcagat cgcttcgccg ctcaaccgcc
     6901 attcgatagc acgttgcacc agggcccgcg cgctcttgag cgacagcttt tccttgatgt
     6961 gggcctgata ggattcgacc gtcttgacgc tcaggcccag ctcctccgcg atctcccgag
     7021 tgccgtagcc ggctccaatc aaatgaaaga cctcgagctc ccggtcgctc aggctggcga
     7081 tcgaggcccg cttgggcttg ctggggccgg tcacgatgcg ttgcagcagg ttggtggtga
     7141 tgcggtcact gacgtacggc tcgcctcgca ggatccggcg cagcaccgtc agtactttgt
     7201 cggttgcctc ctgtttcatc atgtatccgt tggcgcctgc ccgcagggcg cgctccgcgt
     7261 acaccgcctc gtcgtgcatc gagagtacca gaatcggcag gtccggatcc gtgcccctga
     7321 tgctcttgag gaggtccaac ccgtcgcgcc ccggcaggga aatatcgagg acgacgatat
     7381 cggg
//
LOCUS       DASRJQ010000175         2668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2701155, whole genome shotgun sequence.
ACCESSION   DASRJQ010000175 DASRJQ010000000
VERSION     DASRJQ010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2701155"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1335)
                     /locus_tag="VFB89_03965"
     CDS             complement(<1..1335)
                     /locus_tag="VFB89_03965"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZM26486.1"
                     /translation="MLERPLGRGGMATVYLARDLKHDRPVALKVLLPELAATLGPDRF
                     QREIKLAARLQHPHILTVHDSGETGGQLWFTMPYVEGESLRDRLRRERQLPLDDALRI
                     AREAAQALQYAHEHGVVHRDIKPENLLLTKDGNTLVADFGIARALGGEEHLTQTGMSV
                     GTPAYMSPEQASGDKALDARTDVYSLGAVLYEMLAGEPPYTGPTAQQIIMKRFTDPVP
                     SVRKARPSVPESVDQAIQRSLAPLAADRFGTVVEFGRALQIAPGSATPAVTMPAGTPL
                     PTPPTTTAPPTSTVPSIARARKLPVTAIALGLGFLIGAGMLFAWRRTHAGSAETSGPK
                     VLAVLPFENLGDSADAYFADGVTEEVRGKLSRITGLEVIARGSSNQYRHTTKPPQEIA
                     HELGADYLLTATVRWEKSGGGSRVRVTTELADVGPGHAPRTRWQEPFDASLTD"
     gene            2358..>2668
                     /locus_tag="VFB89_03970"
     CDS             2358..>2668
                     /locus_tag="VFB89_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26487.1"
                     /translation="MIRTAIGSDLWPVTWGSDDSLYFSWGDGGGFGGTDALGRVSLGF
                     ARVGGTPPNITETNAWGGYNALTEPTFGGKVWALISVNATLYAIGAVWPGTAGVSTIS
                     SP"
BASE COUNT          429 a          828 c          917 g          494 t
ORIGIN      
        1 gtcagtgagc gacgcgtcga acggctcctg ccagcgggtg cgtggcgcat gccccggccc
       61 gacatcggcc agctcggtgg tgacccgcac ccggcttccg ccgccgctct tctcccatcg
      121 caccgtcgcg gtgagcaggt agtcggcccc cagctcgtgc gcgatctcct gcggcggctt
      181 ggtggtgtgc cggtactggt tggagctgcc gcgggcgatc acctcgagcc cggtgatgcg
      241 cgagagcttg cccctcactt cctcggttac gccatccgcg aaataggcgt cggccgagtc
      301 cccgagattc tcgaacggga gaacggcaag gaccttgggt ccactggtct cggccgagcc
      361 ggcgtgggtg cgccgccagg cgaagagcat gccggcgccg atgaggaagc cgagcccgag
      421 ggcgatcgcg gttaccggga gcttacgtgc tcgggcgatg gaagggactg tcgaggttgg
      481 cggcgcagtc gtcgtcggcg gtgttggaag cggtgtgccg gcgggcatcg tcaccgcggg
      541 cgtcgccgag ccgggcgcga tctgaagcgc gcggccgaac tccaccactg tgccgaaccg
      601 gtcggcggcg aggggcgcca gcgaccgttg aatcgcctgg tccacgctct ccggcacgct
      661 gggccgcgct ttccgcacgc tcggcacggg gtcggtgaac cgcttcatga tgatctgctg
      721 cgctgtgggg ccggtgtacg ggggctcgcc ggccagcatc tcgtagagca cggcgcctag
      781 cgagtagacg tcggtgcgcg catcgagggc cttgtcgccg ctcgcctgct cggggctcat
      841 gtaggcgggc gtgccgacgc tcatgccggt ctgagtcaga tgctcttcgc cacccaatgc
      901 ccgtgcgatc ccgaagtccg cgaccagcgt gttgccatct ttcgtgagca ggagattctc
      961 tggcttgatg tcccggtgca ccacgccgtg ctcgtgcgcg tactggaggg cctgggccgc
     1021 ctcgcgcgcg atccggaggg cgtcgtccag cggaagctgc cgctcgcgcc ggagccggtc
     1081 tcgcagcgac tcgccctcga catagggcat agtgaaccaa agctggccgc cggtctcgcc
     1141 tgaatcgtgg accgtgagga tgtgggggtg ctgaagccgg gcggcaagct tgatctcgcg
     1201 ctggaagcgg tcggggccga gcgtcgcggc aagctcgggc aggaggactt ttagggcgac
     1261 cgggcggtcg tgcttgaggt cgcgcgccag gtagaccgtt gccatgccac cgcgcccgag
     1321 cggacgctca agcacgtaac gatcgcgcag accggcaact agcgcggtcg ttgaatccgt
     1381 ggacgacgag ggggtagtga tgcagaaatc tggccggggg cgccgcgggc cgccaggcta
     1441 ctagcggcgc acgctcgagg ttactaccgc acggcgcgac tccagcccga ctgtcgattc
     1501 cgaacggtag tatcacccgc tgccaggcag ctcgatccga taggtggccg ggttgggccc
     1561 tgcgcggcgg ttgtgcaagg cgaggaatac ggcaatactg ggctagttaa cgacggcagg
     1621 ctcgcctatg tcgcttggtg cgaagtcaat gagcgacttc gcccaggtcc gctcttcgtc
     1681 gccggcttgg gcgcgatcga gtgtctctac gcgactcatc gtggaccgga ccgcttctat
     1741 gattccgagg tgtcctttga aggggcgcgc catctggccg ggcgggcggg gctgcgtgca
     1801 gcatccgccc gctgactgca cgatctacga gctggtccgt gagcggcacc cgtttcatcg
     1861 aggcgcggcc cctcctgtga aagccttggt tacaggcgct accggcttca ttggtggtca
     1921 tctgtgtctc gtgaaacgtg acggtgaggt cgtcccgcat cgcccggaag aactgataac
     1981 actggcccgc agacttgaac ggagactcaa tggttaccgt tcttgaatca gtggtcttgc
     2041 tggcgacatg gggcacaagc gtgacgcgcg ggcaaagtcc gactccagcg cctccgcctc
     2101 gctcgcttcc tgcgtgcgcc accaccctgc ctacgctcaa tcctcatatg ggaaccagac
     2161 ttggttgcac aatcagtttc gatacttctg tagtgtcgtc ggcctatcgg tctgttacgc
     2221 ctcttgacct agatgaagtc ggagaaggac tggtcgatgt caactacatc acgcctcagt
     2281 cgagaaccat tccggcccta cctcctcgat cagcatcgtc gacaatcatt tccgggatca
     2341 cgtgggattc cgccagcgtg attcgtaccg ccatcggcag cgatttgtgg ccggtgacct
     2401 ggggttctga cgacagtctg tatttcagct ggggagacgg cgggggattc ggcgggaccg
     2461 acgcgcttgg ccgggtaagt cttggatttg cgcgggtcgg aggaacacca cccaatatca
     2521 cagaaacaaa tgcgtggggc ggctacaacg cgctcaccga gccgaccttt ggaggaaaag
     2581 tgtgggcgct gatctcggtc aacgcgacgc tctatgctat aggagccgtg tggccgggaa
     2641 cggctggagt aagcacaatc agttctcc
//
LOCUS       DASRJQ010000176         2193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_160685, whole genome shotgun sequence.
ACCESSION   DASRJQ010000176 DASRJQ010000000
VERSION     DASRJQ010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160685"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..1635)
                     /gene="dnaA"
                     /locus_tag="VFB89_03975"
     CDS             complement(250..1635)
                     /gene="dnaA"
                     /locus_tag="VFB89_03975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HZM26488.1"
                     /translation="MQLSLKEAWTRILDDARREIPEKDFKAWVEPAKPVSLDGDRLAI
                     GVPDEFAVQWNEAKNSHLFSRLAERALGRPVSVVFRVDEERLQRPQMDFFVAPIPQGN
                     AGAAPNPAAQPLNERYTFENFVIGKSNELAAAAAHAVAEAPGKLYNPLFIYGATGLGK
                     THLMQAIGHIVTRRSPSVRVLYVGAEQFINEVIESIHSRTMPELRRRYRNDVDLFLVD
                     DVHFLEGKEATQEEFFHTFNTLYEAKKQIVLTSDRPPKEMPGLEARLVSRFEWGMVAD
                     VGQPDLEHRIAILRKKQEQDHLETTIPDEVLSFIAEYVHSNVRELEGCIIKLLLYASL
                     RHREITIDLAREALADKIRPGQEQASSMPKLNPTIDKVQDVVARRWGVTPEGLRSKAR
                     IKTLTVPRQIAMYLAREVLQMQLVEIGQAFGGRDHSTVIHSVDKVQRQLQRDRQFRER
                     VESARQELYAP"
     gene            1776..1937
                     /gene="rpmH"
                     /locus_tag="VFB89_03980"
     CDS             1776..1937
                     /gene="rpmH"
                     /locus_tag="VFB89_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HZM26489.1"
                     /translation="MQPTYRPRNRKRINRHGFRARMADRWGREVLSRRRKKGRKRLTV
                     RVPSKHGGS"
BASE COUNT          392 a          683 c          697 g          421 t
ORIGIN      
        1 tttttcccgc gtgatggtga acttcatccg aacgctcccc gcaaagccat ataaaaggaa
       61 ctactaatag atagttatac ctagtagtcg tagtagaggg tctggatatg tggatgcggc
      121 gggcaactgc cgatcccact ccctcctaga ccattctgac cgcgtcgaca ccatgtgggt
      181 gatggggaac gcagtgtaca actcccccgc ttatcccagt cgtccacagc cccgtatgcg
      241 tcgcagcgat tacggtgcgt acaactcctg tcgagccgac tcaacgcgtt cgcggaactg
      301 gcgatcgcgc tgcaattgtc tctgcacctt gtccacactg tgaatcacgg tcgagtgatc
      361 gcggccgccg aaggcctggc cgatctccac gagttgcatc tggagcacct cgcgagccag
      421 atacatcgcg atctgacgcg gcaccgtgag cgtcttgatg cgagccttcg accggagccc
      481 ttccggcgtg acgccccagc gtcgcgcgac cacgtcctgc accttatcga tggtcggatt
      541 cagcttgggc atggagctgg cctgctcctg cccgggccgg atcttgtcgg ccagcgcctc
      601 gcgcgccagg tcgatggtga tttcacgatg ccgaagcgac gcgtagagca ggagcttgat
      661 gatgcagccc tccagctccc gcacattgga atgcacgtac tccgcgatga agctcaagac
      721 ctcatcaggt atcgtggttt cgaggtggtc ctgctcctgt ttcttccgga ggatggcgat
      781 ccggtgctcc aggtcgggct ggcccacgtc cgccaccatc ccccactcga accggctgac
      841 caggcgggcc tccagcccgg gcatctcctt cgggggacga tccgacgtga ggacgatctg
      901 tttcttggcc tcgtacagcg tgttgaacgt atgaaagaac tcttcctggg tggcttcctt
      961 gccctcgagg aagtgcacgt cgtccaccag gaacaggtcc acatcgttgc gatagcgccg
     1021 gcggagctcc ggcatggtgc gggagtggat gctctcgatc acctcgttga tgaactgctc
     1081 ggcgccgaca tagagcaccc gcacagaggg gcttcgccgg gtgacgatat gcccgatggc
     1141 ctgcatcaaa tgcgtcttgc ccagcccggt ggccccgtag atgaagagcg ggttgtagag
     1201 cttcccgggg gcctcggcga cggcgtgtgc ggccgccgcg gcgagctcgt tggacttgcc
     1261 gatgacgaaa ttctcgaagg tgtagcgctc attcagaggc tgcgcggcgg gattcggcgc
     1321 ggcaccggcg ttgccctgcg gaatcggggc gacgaaaaaa tccatttgcg gtcgctgcaa
     1381 ccgctcttcg tccacccgga acaccaccga gacgggacgg cccagcgcgc gctcggcaag
     1441 cctcgagaag agatgggaat tcttggcttc gttccactgg acggcgaatt cgtcggggac
     1501 gccgatcgcc aatcgatcgc cgtcgagcga gaccggtttg gcgggctcga cccacgcctt
     1561 aaaatccttc tccgggattt cgcgccgggc gtcatccagg atgcgggtcc aggcctcttt
     1621 gagcgacagc tgcatgccga tcgtttcggt ctcgatggcg gtagggatgt tgttggggca
     1681 ggcgaagcta cggcacggtt gtggaaaagt caaccttgac cgaaacaggt tatcgcgggt
     1741 aactttccgg gctcactgag tgctaaggaa ttgtcatgca gccgacctat cgccctcgca
     1801 atcgcaagcg gatcaaccgc cacggtttcc gggcccgcat ggccgatcgc tggggccgcg
     1861 aggtgctttc ccgccgccgc aagaagggcc gcaagcggct cacggtcaga gttccttcga
     1921 agcacggggg aagctgacgg tagccgagcg gctgccgcgc gccggccggc ttacccgctc
     1981 agccctgcta cgccgagtgc aggaaaccgg gaaacggcgg cgcctgccgc acctcgatgt
     2041 cctatggaca gacaatcaag cgggccatcc cagggtggga ctgatcgttc cgaagttcca
     2101 gtccaccgcg gtggcccgca accgtttgcg gcgatggctg cgagaggtct ggcgccggca
     2161 gctggtagcg ggcgtgccgc cgtgggacgt ggt
//
LOCUS       DASRJQ010000177         4460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_446223, whole genome shotgun sequence.
ACCESSION   DASRJQ010000177 DASRJQ010000000
VERSION     DASRJQ010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446223"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VFB89_03985"
     CDS             complement(<1..320)
                     /locus_tag="VFB89_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZM26490.1"
                     /translation="MAAKQKAGNQRSNTQVKVGASQLGQRRYPAPPFPKQHQRRPGSE
                     AALKPRPQFEAPQYRGSGKLDGLVALITGGDSGIGRAVAVLFAREGADVALVYLSETK
                     DAEET"
     gene            566..1057
                     /locus_tag="VFB89_03990"
     CDS             566..1057
                     /locus_tag="VFB89_03990"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HZM26491.1"
                     /translation="MAASPRDRDEQEEVPLSKAAQYLLEECRMVLPGIQALFGFQLIA
                     VFNQSFAQKLTGAEQRLHLLAIGLVAVAIALIMAPAAYHRQTAPQHVSSAFIDVSTRL
                     LLWSMLPLAASICVEFYLISRVILNSSYAALPAAVLFLIFVALWFVLPRMRALRKAIA
                     RRN"
     gene            complement(1151..1819)
                     /locus_tag="VFB89_03995"
     CDS             complement(1151..1819)
                     /locus_tag="VFB89_03995"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HZM26492.1"
                     /translation="MRLMLTAVVLQAVLVGTAAAQAHRPGAGAGIHAPASPQPTGFML
                     NAHTVAAFGTSAGDATQLDDHPITTGLGPGGGVQVGYAFNRTVMVFAGADIAKQNSTR
                     QDLPGNLGLTHIDLGTRVNIPVHSSRVLPYAMAAVEHRSLGANVEDDVTGDQTKVSFS
                     GFSLGVGGGVQYFMSPKLALDAGVRVGFGKLGTLKVDGQKEQTPQLNNSTTPSLLFGV
                     NWYP"
     gene            complement(1851..2420)
                     /locus_tag="VFB89_04000"
     CDS             complement(1851..2420)
                     /locus_tag="VFB89_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26493.1"
                     /translation="MRRFSLLVSAALCLALLACGGGGDALGPDGHHPDPGTPQEPGNP
                     GTPGTPDPGTPQIPNDPNPAPPVAGGYTLGLINDANPGQIVLLSNPDGSVIGLYRFVA
                     ASTLILDPLGGYTLTLTYSDEQGSYTLEDKGQWKGGNQDQDGNIHLSFKSDSWGDEFE
                     GLAKNSKVEIRYDMDGDGALDTLYGFVRQ"
     gene            complement(2600..>4460)
                     /locus_tag="VFB89_04005"
     CDS             complement(2600..>4460)
                     /locus_tag="VFB89_04005"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HZM26494.1"
                     /translation="MVSRSWRGLASLAAGDLPRARRETLGTADYFTREGDLENQSEIQ
                     RALAEIAMRERDWPAAEEALDKAEVFLRKLGTPAARTGQLFERGRLALYRGDSVSAQK
                     LFAGYLASLDSSAHLKRFEARTYLGDAYARLGRLDVAERQLGEASDELDRWRATLSAQ
                     DLRVSAFQAGASAQNDRNAAVARVLAALAQGGRADAAFTLAERRRARELVDRLVQNAA
                     LETSHNDAGDRLSREVAPVTPADVAALLPDNRTAFLEYVTGARGAPTTVFVITRGRQG
                     PPVAQARILRPADSLTGDIGRFAALVVRRAETPRDAQALGAALLDPVVALLDPSVTRL
                     VIVPDGSLHRVPWDALRLQDGRYLVERYAVGIAPSAGVLAQLLRRARAPTAVPSRLLA
                     FGDPIFPGEASGNASVARDAALEGFHSAFAATGGLPRLQGSGREVRSIARYAPGAELK
                     VREAASESYLLKSSLAQFRIIHFATHALVDDRVSFRTALALAPGGGEDGFVTPAELAR
                     LRLDADLVVLSACRTAGGVVVDGEGVQGLTAPLLAAGARSIVATGWRVGDERIVSLIE
                     RFYAELARGRPVAEALRAAKLAAIERKAPASIWAAFTVFGDPFVTVSLRPPAS"
BASE COUNT          746 a         1466 c         1476 g          772 t
ORIGIN      
        1 gtttcttcgg cgtccttcgt ctcgctcaaa tagacgagcg caacatccgc cccttcgcgg
       61 gcgaacagga ctgcgacggc gcggccgatc cccgagtcac cacccgtaat gagggcaacc
      121 aacccgtcaa gcttgcccga gcctcgatat tgcggcgctt cgaactgcgg cctcggctta
      181 agtgccgcct cgctgccggg cctcctctgg tgctgtttcg ggaagggcgg cgcggggtag
      241 cgacgctgcc ccagctggga ggcgccgact ttgacctggg tattcgaccg ctgatttccc
      301 gccttctgtt ttgctgccac gcggactcct ggttgtcgaa gaaaaaacga tggagttcgg
      361 gtcgtaccca atgatgtgac tgttctcaca cacaggcctc ggtgcgacac ggcttccccc
      421 ctcgaggagg ccgacgcagt gccgtcgtac cggtcgtgat tgggcgcacc gcagcggtcg
      481 actctcaaac ggcattgtgg cgtccgctca caagggcggg cggcgcggta gacgcctgtt
      541 cttttcattt ggaggaagga tcgtggtggc cgcaagtcct cgcgatcgtg acgagcagga
      601 agaggtacct ctttcaaagg cagctcaata cctcctggag gagtgccgca tggtgctgcc
      661 aggcatccag gccctcttcg gatttcagtt gatcgccgtt tttaatcaga gttttgctca
      721 gaaactgacc ggggccgaac agcggcttca tttgttggcc ataggcctcg tcgcggttgc
      781 aatcgcgctc atcatggcgc ccgcggcgta tcaccggcag accgccccac agcatgtgag
      841 cagcgcattt atcgatgtct cgacccgcct cctgctgtgg agcatgcttc cgttggcagc
      901 cagtatctgc gtcgagttct atctcatcag ccgggtgatt ctgaatagct cttatgccgc
      961 cctgccagca gcggtgttgt ttctcatttt cgttgcgttg tggttcgtgc ttccgcgtat
     1021 gcgagccttg aggaaagcca tagctcgccg caactgatgc gtcgcccgcg gaagggggct
     1081 gcctgagacg ccgaaggcac ccctccttct caggtagggt gcccaggcgt cttccaactc
     1141 cgctccgctc tcaggggtac cagttcactc cgaacaagag actgggcgtc gtgctgttgt
     1201 tgagctgggg cgtttgctcc ttctgcccgt cgaccttgag cgtgcccagc tttccgaaac
     1261 caactctgac gcccgcatcg agcgcgagct tgggtgacat gaagtactga acgccacccc
     1321 cgaccccgag cgaaaagccg gagaagctca ccttggtctg gtctccagtg acatcgtctt
     1381 cgacgttggc gccgaggctc cggtgctcga ctgcggccat ggcatagggc aggacacgtg
     1441 aagagtgcac cgggatgttg actcgggttc cgagatcgat gtgagtgagg ccgagatttc
     1501 ccggcaggtc ctgccgcgtg ctgttctgct tggcgatgtc cgcgcccgcg aacaccatca
     1561 cggtccggtt gaacgcgtaa ccaacctgca ctccgccgcc cggcccgaga ccggtcgtga
     1621 tcggatggtc gtccagctgg gtcgcgtccc cagccgaggt gccgaaggcc gcaaccgtgt
     1681 gagcgttgag catgaagccg gtcggctgcg ggctggccgg ggcgtggata ccggcaccgg
     1741 cacccgggcg atgcgcctgt gcggctgccg tgccgacgag caccgcttgc agcaccaccg
     1801 cagtaagcat gagtcgcatg atagagtctc ctcgagtcga tgagcgtggg ttactgccgc
     1861 acgaagccgt agagcgtgtc gagggcgccg tcgccatcca tgtcatacct gatctcgacc
     1921 ttgctgttct tggccaggcc ctcgaactcg tcaccccacg agtcggactt gaagctcagg
     1981 tggatgttgc cgtcctggtc ttgattcccg cccttccatt ggcccttgtc ctcgagcgtg
     2041 tacgagccct gttcgtctga gtacgtgagc gtgagggtat agccgccgag cggatcgagg
     2101 atcagcgtgc tggccgcgac aaagcgatac agaccgatga cgcttccatc cgggttcgac
     2161 agcagcacga tctgtccggg gttcgcatcg ttgatgagcc cgagcgtgta gccgccggcg
     2221 accggaggcg cgggattagg gtcgttcggg atctgcggag tgccgggatc gggagtgccg
     2281 ggagtgccgg gattgcctgg ctcctgcggc gttccgggat ccgggtgatg gccatcgggg
     2341 ccgagggcat ctccgccgcc cccgcaggcg agcagcgcga gacacagcgc ggcagatacg
     2401 agaagtgaga agcggcgcac cattgaactc ctccgagtga gtggtctgtc gttcggaggt
     2461 aggtacgcag ctaggcgcga cgcagtctca cgcagggtgt acgcgtggga gcgcacaccg
     2521 agtcgggtcg tcaggctgaa acgcgagggc ctttagttat gacgcaggga aaacgacgct
     2581 cggctcagag gatggtaaat cagctcgccg gcggccgcag cgaaacggtc acgaacggat
     2641 cgccgaacac ggtaaaagcc gcccagattg acgccggcgc cttccgttcg atcgcggcga
     2701 gcttcgcggc acgcaacgcc tcagccacgg ggcgcccccg cgccagctcg gcgtagaagc
     2761 gttcgatcag agacacaatg cgctcgtcgc ccacccgcca cccggtggcc acgatgcttc
     2821 tcgcgccggc ggcgagcagc ggcgcagtga ggccctgcac accttcgccg tccaccacca
     2881 cgccaccggc cgtgcggcag gcggacagga ctacgaggtc ggcatcgagt cgaagccgtg
     2941 ccagctcggc gggagtgacg aagccgtctt cgccaccgcc gggagcaagt gccaacgccg
     3001 tgcggaacga gacccggtcg tccaccagtg cgtgggtcgc gaagtgaatg atccggaact
     3061 gtgcgaggga agacttgagc aggtagctct cgctcgccgc ctctcgcacc ttcagctcgg
     3121 cgcctggcgc gtagcgggcg atcgagcgca cctcgcggcc cgacccctgg aggcggggca
     3181 gaccgccggt cgcggcgaat gcagagtgaa aaccttccag cgccgcatcg cgcgccaccg
     3241 acgcattgcc gctggcctct cccggaaaga tcggatctcc gaacgcgagc agtcgcgagg
     3301 gcacggcggt gggcgcgcgc gcgcgacgga gtagttgtgc cagcacgccg gccgatggcg
     3361 caatgcccac cgcgtagcgc tccaccaggt atcggccatc ctgcaggcgc agcgcgtccc
     3421 aaggtacccg gtgcagcgag ccatcgggca cgatgacaag acgggtgacc gacggatcga
     3481 gcagcgcgac cacaggatcg aggagcgcag caccgagcgc ctgcgcgtcg cgcggcgttt
     3541 cggcgcgcct gaccacgagc gccgcgaacc ggccgatgtc gccggtgagc gagtcggccg
     3601 gccggaggat ccgggcctgt gccactgggg gtccttgtcg accacgagtg atcacaaaca
     3661 ccgtggtcgg cgctccgcgc gcaccggtca cgtactcgag gaaagcagtg cggttgtccg
     3721 gcagcagcgc cgcgacgtcg gcaggagtga ccggcgcaac ctcccgactg agtcggtcgc
     3781 cggcgtcgtt atggctcgtc tccagcgccg cgttctgcac caggcggtcc accagctcgc
     3841 gcgcacgccg tcgttccgcc agcgtgaagg ccgcatcagc tcgcccgccc tgtgccaagg
     3901 ccgcgagcac ccgggccacg gcggcattcc gatcattctg ggccgaggcg ccggcctgga
     3961 acgccgaaac gcggaggtcc tgtgcgctca gagtcgcgcg ccagcggtcc agctcgtccg
     4021 aggcctcgcc cagctgtcgc tcggcgacat cgagccggcc gagccgtgcg taggcatcgc
     4081 ccagataggt ccgtgcctcg aatcgcttga ggtgggcgct cgagtcgagg ctggccaggt
     4141 atccggcaaa gagtttctga gccgagaccg agtcgccccg atacagagcg agacggcccc
     4201 gctcgaacag ctgccccgtt ctcgccgccg gagtgcccag cttgcggaga aatacctctg
     4261 ccttgtccag cgcctcctcg gcggccggcc aatcgcgctc gcgcatggcg atctcggcca
     4321 gtgcccgctg gatctcggat tgattctcga gatcgccctc gcgggtgaag tagtcggccg
     4381 tcccgagcgt ctcacgccgc gcccgaggga gatcacccgc agccaggctg gccaggccgc
     4441 gccacgagcg gctcaccatg
//
LOCUS       DASRJQ010000178         3867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3063531, whole genome shotgun sequence.
ACCESSION   DASRJQ010000178 DASRJQ010000000
VERSION     DASRJQ010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3063531"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2112
                     /locus_tag="VFB89_04010"
     CDS             <1..2112
                     /locus_tag="VFB89_04010"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26495.1"
                     /translation="AAGETDRARHRVAEAREREALLRSQRRQAEEALAQTTARREALQ
                     ELERERVGLAPGAAALLAARDQFGDAVLGPLSDFVRTPPEHAELAERLLGGWTHAILV
                     RDAAAVEAIRGWHAEQQPGALVLLPADPGPQAPVGAPPTDGRLEAEGPAAAWLRTALA
                     GSQVLDGTGRVLRRASGAILLGGAAAPSGPLRRRAELENLVHEAELASIALGSAEADL
                     GAALARLATLEEALTHSTGAVERARETEHAALAAQDDTVRFVNNLARELATADAEVAR
                     LTDRIERAEQRLVEVDVALVEGDVARARLGEELGTARAGLSELEISQEGTREKRAQWQ
                     VQEAHVAARLRAATERWERAQAAQVEAETGIRALTDELAQLHLDCTALSAQQAQWQEA
                     RAERRVALLELETADTEAEAALEAAERALEWAERDVTTQRSLVEAHGEESHRLQIELT
                     EATGLRRNIVERIEAEWRKPIDQLLEEAPRLELDLETLESEAIRIASALEAIGPVNPL
                     AMEEHAEEVKRFEFLSGQRDDLVSARQSLLQAIREIDGTARAMFLETFEAVKANFHTV
                     FDTLFGGGECDLRLANPDDPLESEIEILAAPRGKRIQHVRLLSMGERTLVATSLLFGL
                     YLTKPSPFCLMDEVDAPLDDANVGRFTRLLDEFKRETQFIVITHNPRTMHAADAVYGV
                     TMQEPGVSTIVGVRLGETVGV"
     gene            2310..3350
                     /gene="aroF"
                     /locus_tag="VFB89_04015"
     CDS             2310..3350
                     /gene="aroF"
                     /locus_tag="VFB89_04015"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HZM26496.1"
                     /translation="MLVVMRHGASEQEIQQVAAAIEQMGYEARIMPGRQRTTVGLVGN
                     DGRVDPSRLAGLPGVQECIPVTKPYKQVSREWRAQPTVVQFPGGLTIGGDEVVVMAGP
                     CSVESDTQILEAAHAVREAGATMLRGGAFKPRSSPYSFQGLGRKGLELLARAREETGL
                     LIVTEAMDPEGLDAVAEIADLVQIGARNMQNYSLLKKAGRSRKPVLLKRGLAATIQEL
                     LLSAEYILAEGNPNVILCERGVRTFDSATRNLFDLAAVAVVHGLSHLPIVADPSHGTG
                     HRDMVIPMARAAIAAGADGLLIEVHPNPDAALSDGAQSLYPAQFSQLMREVRLIAEVI
                     GRRVAEPVGMRA"
     gene            3347..>3867
                     /locus_tag="VFB89_04020"
     CDS             3347..>3867
                     /locus_tag="VFB89_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HZM26497.1"
                     /translation="MRSLYTITALSLLLSPAAAPLSAQQDARSIVSRAAFAYRSLSSL
                     RADFDQLIENQMIGNAASKGVLEQAGQARLSMRFTDPPGEAIVIDGKWVWVYTPSTTP
                     GQVLRIPVPSGGPVYGYNLLAWLMDRPTERYNSTYVRADRLDNRPMDVVEMVPTVPDM
                     PFGRATVWLDRGDG"
BASE COUNT          685 a         1244 c         1327 g          611 t
ORIGIN      
        1 gccgcgggtg aaaccgatcg tgcgcgccat cgggttgcgg aagcgcgcga gcgcgaggca
       61 ctgctgcggt cgcagcgccg ccaggccgaa gaggctctgg ctcagacgac agcgcgtcgc
      121 gaggcgctgc aggaattaga gcgggaacga gtcggcctcg caccgggtgc agcggcgctc
      181 ctggcggcgc gggatcagtt cggtgacgcc gtactcggcc cgctcagcga cttcgtacgt
      241 acaccaccgg aacatgcgga gctggcggag cgactgttgg gcggatggac ccacgcgatt
      301 ctggtccgag atgccgccgc cgttgaagcc atccgcggct ggcatgcgga gcagcagcct
      361 ggagccctgg tgctgctgcc cgcggacccc ggtccgcaag ctccggtggg cgccccaccg
      421 accgatggca gactcgaagc cgaggggccg gcagccgcct ggctccggac ggccctcgca
      481 gggtcgcagg ttctcgatgg tacggggcgg gttctccgcc gagccagcgg ggcaatcctt
      541 ctcggcggtg ccgccgctcc gtccggcccg ctgcgccgcc gggccgagtt ggagaacctg
      601 gtgcacgaag ccgagcttgc cagcattgcc cttggctcgg ccgaggcgga cctgggtgcg
      661 gccctggccc ggctcgcgac tctggaagag gcgctcaccc actcgaccgg tgcagtggag
      721 cgcgcgcggg aaaccgaaca tgcggcgctc gcggcccaag acgacaccgt gcgcttcgtc
      781 aacaacctcg caagggagct ggccacggca gatgcggaag tcgctcggct tactgatcgc
      841 atcgagcggg ccgaacagcg tctggttgaa gtggatgtcg ccctggtcga gggcgacgtc
      901 gcccgggcgc gcctgggcga agagctggga accgcccgcg ccggcttgtc cgagctcgag
      961 atctcgcagg aaggtacccg cgagaagcgc gctcaatggc aggttcagga ggcgcacgtc
     1021 gcggctcggc ttcgcgcggc cacggaacgg tgggagagag cccaggcggc tcaggtcgag
     1081 gccgagaccg gcatccgcgc gctcactgac gagctggcgc agctccatct cgactgcacc
     1141 gcgctctcgg ctcagcaagc gcagtggcag gaggcgcggg ccgagcgtcg tgtggcgctg
     1201 ctcgagctgg aaacggcgga taccgaggcg gaagctgccc tggaagcagc agaacgggct
     1261 ctcgagtggg ccgagcggga tgtaacgacg cagcgcagcc tggtggaggc ccatggcgag
     1321 gagagccacc ggctgcaaat cgagctgacc gaagcgacag gcctccggcg caacattgtc
     1381 gagcgtatcg aagccgagtg gcggaagccg attgatcagc tgctggaaga ggcaccccgg
     1441 ctcgagctcg atctggagac gctggagtcg gaggccattc ggattgcgag tgcgctcgaa
     1501 gcgatcggac cagtcaaccc cctggcgatg gaagagcacg ccgaggaggt caaacggttc
     1561 gagtttctct cgggacagcg ggacgatctg gtcagcgccc gccagtcgct gctacaggcg
     1621 attcgagaga tcgatggcac cgcgcgcgcc atgttccttg aaacattcga agccgtcaaa
     1681 gcgaacttcc acacggtgtt cgacacgctg ttcggcggcg gcgagtgcga tcttcggttg
     1741 gccaatccgg acgatccgct ggagagcgaa atcgaaatcc tggccgcacc gcggggtaag
     1801 cggatccagc acgtgcgtct gctgtccatg ggcgagcgga cgctggtcgc gacgtcgctc
     1861 ctcttcgggc tctatctcac caagccgagc cccttctgcc tgatggacga ggtggacgcg
     1921 ccgctcgacg acgccaacgt ggggcgcttc acccgtctcc tcgacgaatt caagcgcgag
     1981 acccagttta tcgtcatcac tcacaatcca cgtaccatgc acgcggccga tgcggtgtac
     2041 ggggtgacga tgcaggagcc gggtgtatcg acgatcgtgg gagtgcggtt gggagagacg
     2101 gtgggggttt gagctagcag ctatcagcta ccaactatca gctatcagat ggcgtgaacg
     2161 cgtatctgtg acaccgagta gcgcgtgtgc cacttccagt gtgccttgca accagcgcga
     2221 aatacgagcg cggctaccca gcgactgata gctgatgctg atagctgata gctgactagc
     2281 ttacacccca tatccaacgg gacaccctga tgctcgttgt catgcgccac ggcgccagcg
     2341 aacaggaaat ccagcaggtc gccgcggcca tcgagcagat gggctacgaa gcgcgcatca
     2401 tgccaggccg gcagcggact actgtcggcc tggtcggcaa cgacgggcgg gtggacccat
     2461 cgcggttggc cggcctcccc ggcgtgcagg aatgcattcc cgtcacgaag ccctacaagc
     2521 aggtctcgcg cgagtggcgc gcccagccca ctgtcgtcca gttccctggt ggtctcacca
     2581 ttggtggcga tgaggtggtg gtcatggccg ggccgtgctc ggtcgaaagc gacacccaga
     2641 ttctggaggc cgcccacgcc gtccgggaag ccggcgccac gatgctacgc gggggtgcct
     2701 tcaagccgcg cagctcaccg tactcgtttc aggggctcgg ccggaagggg ctggagctgc
     2761 tcgctcgtgc ccgcgaagag acggggttgt tgatcgtgac cgaagcgatg gaccccgaag
     2821 ggctggacgc agtcgccgaa attgcggacc tggtccagat cggggcccgc aacatgcaga
     2881 actactccct gctcaagaag gcgggccgct cccgcaagcc ggtcctgctc aagcggggcc
     2941 ttgcagccac gattcaggag ctactgctct cggccgagta catcctcgcc gaaggcaacc
     3001 caaatgtgat tctctgcgag cgaggcgtgc gcacgttcga ctctgccacc cgtaacctgt
     3061 tcgacctcgc ggctgtcgcc gtcgtccacg gcttgtcgca cttgcccatc gtggccgatc
     3121 cgagccacgg caccggtcac cgcgacatgg tgatcccgat ggcgcgcgcc gcgatcgcgg
     3181 ccggtgctga cgggctcctg atcgaggtgc accccaaccc cgacgctgcc ctttccgacg
     3241 gagcccagag tctctatccg gcgcaattca gccagttgat gcgcgaagtg cggctgattg
     3301 ccgaggtaat cgggcggcgg gtcgcggagc cggtgggaat gcgcgcgtga gaagcctcta
     3361 taccatcacc gctttgagtc tgctgctgtc cccggcggct gccccgctgt ccgcccagca
     3421 ggacgcgcga tccatcgtga gccgggccgc gtttgcctat agatcactct cttcactacg
     3481 ggccgacttc gatcagctga tcgaaaacca gatgatcggc aacgccgcta gcaaaggggt
     3541 gctggaacaa gccggacagg cgcgcctgtc gatgcggttc accgacccgc ccggcgaagc
     3601 catcgtcatc gatgggaaat gggtgtgggt ctacaccccg agtaccacac cgggccaggt
     3661 gctccggatt ccggtaccaa gcggcggacc ggtgtacggg tacaatctgc tggcgtggct
     3721 gatggaccgc ccgacggagc gatacaactc gacctacgtc cgcgccgacc gactcgacaa
     3781 ccgcccgatg gacgtggtcg agatggttcc cacggtgccc gatatgccct tcggacgtgc
     3841 aaccgtatgg ctggatcggg gcgacgg
//
LOCUS       DASRJQ010000179         3540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2125381, whole genome shotgun sequence.
ACCESSION   DASRJQ010000179 DASRJQ010000000
VERSION     DASRJQ010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2125381"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..715
                     /gene="dps"
                     /locus_tag="VFB89_04025"
                     /gene_synonym="pexB"
     CDS             113..715
                     /gene="dps"
                     /locus_tag="VFB89_04025"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: protein motif:HMM:NF006975.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HZM26498.1"
                     /translation="MARTATARPPAGSASRTSREPARPRTTRIYPTKNDLPEASRVEA
                     IDLLNQRLADCIDLQTQCKQAHWNVKGPSFIALHKLFDEVNEAVEEYVDLIAERVVQL
                     GGIAEGTARIVAQRSGLVDYPLTLASGDEHVAALSDVLAEFGRTARLGIDEMNDLQDA
                     DSADILTEISRGIDQWLWFVEAHQQGKTVAVEAGRDNGRS"
     gene            complement(712..2175)
                     /locus_tag="VFB89_04030"
     CDS             complement(712..2175)
                     /locus_tag="VFB89_04030"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZM26499.1"
                     /translation="MTRCFLLLVDGLRRDLAETELAAGNLPNLAAMVEDGGRSCAITA
                     FPSTTSVAYLPFLTGCMPGRCDVPSIRWLDRRHYAGRWWRDRNAVRSYCGYQAGMLDA
                     DIAPNVRTIFELVPHSLGLFTPVARGLSSDRDPTRASRKFWGILAHYAEWHQPSDDAV
                     ARYLIDAAGGLARFVFAQFPAVDGYAHQDGPMGPLVRRSLRKLDDVVGRMVQTLRARG
                     ELERSLILLVSDHGSAAVHTHLDLADWFRDRSVPVLAHPVIWRRNPRAAVMVAGNGSA
                     MVYARPTEPRERRLPLSRLRRPEAFGTRQDILEALLAEEAVGLVAAEEEDGGVRVASA
                     DGEAVLQRLGPGISYRPITGDPLSIGRPWLATERAWLEATWDAEYPDAAYQLMDQFRS
                     ARTGDMVVAARAGFDFRDRWEIPEHRAGHGSLTRDHMQTPVWSSVPLPEGPIRTVDLF
                     PAMLRWLGVELPRVVDGAAPWLPTPGPATGRRPVVVG"
     gene            complement(2238..3203)
                     /locus_tag="VFB89_04035"
     CDS             complement(2238..3203)
                     /locus_tag="VFB89_04035"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HZM26500.1"
                     /translation="MTREGTSAGRFSLILFGLAAAFSLTPWATPAIGLALGAALALTV
                     GNPVPRLTGRAARWLLQTAVVGLGFGMPFSAVLAAGQMGVGYTVAGIATALVLGLLIG
                     SWLRIEQKTSFLITAGTSICGGSAIAAVGPAIGARPEAMSVSLATIFLLNAVALYLFP
                     PVGRMLHLSQHQFAIWAAVAIHDTSSVVGAASSYGPLALEQATVLKLARALWIVPLTV
                     AAAIVARRSGSATTTRVPLPWFIGLFALAAFSRSVLPPSTAPVLDDIADAARIALVLT
                     LFLIGATLTRSTLKRLGPRPFLQGVLLWGAVGGLSLLVAARWVAP"
     gene            complement(3330..3404)
                     /locus_tag="VFB89_04040"
     tRNA            complement(3330..3404)
                     /locus_tag="VFB89_04040"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3367..3369),aa:Pro,seq:cgg)
     gene            complement(3413..3486)
                     /locus_tag="VFB89_04045"
     tRNA            complement(3413..3486)
                     /locus_tag="VFB89_04045"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3450..3452),aa:Arg,seq:acg)
BASE COUNT          688 a         1177 c         1162 g          513 t
ORIGIN      
        1 gccagtcggg gtgcggaggt gacttgtacc cccgctgaat ttgacccgcc ggagcacatt
       61 gctcgaaggc aggaaacagg gaaaccgcac caaaatccaa taggagcagt acatggcacg
      121 cacggccaca gcccgtcccc ccgccggaag cgcgagccgc acttcgcgcg agccggctcg
      181 gccgcggacg acacggatct accccaccaa gaacgacctc cccgaagcca gccgggtcga
      241 ggccatcgac ctcctcaatc aacgcctggc cgattgcatc gatctccaga cccaatgcaa
      301 gcaggcccat tggaacgtca aaggaccgag cttcatcgca ctccacaagc tcttcgacga
      361 ggtgaacgaa gcggttgagg agtacgtgga tctcatcgcg gagcgagtgg tccaactggg
      421 cggcattgcc gaggggaccg ctcgcatcgt ggcgcaacgc agcggcctgg tcgactatcc
      481 actgaccctg gcgtcaggcg atgagcatgt cgcggctctc tcggatgtgc tcgccgagtt
      541 cggtcgcacc gcccgtctgg gtatcgacga aatgaacgac ctccaggatg ctgacagcgc
      601 cgacatcctc accgagatct ctcgcggtat cgatcagtgg ctctggttcg tggaagcgca
      661 ccagcagggc aagacggtgg cggtcgaagc cggccgcgac aacggccgga gctagccgac
      721 cactacgggg cgccttccgg ttgctggccc tggggtaggc agccaggggg ccgccccgtc
      781 caccacgcgc ggaagttcga cgcccagcca ccgcaacatg gccggaaaga gatccacggt
      841 ccggatcggc ccctccggaa gcggcacgct ggaccacacc ggcgtctgca tgtgatcgcg
      901 ggtcaggctc ccgtgacccg cgcggtgctc cgggatttcc cagcgatcgc ggaaatcgaa
      961 gccggcgcgg gcggcgacga ccatatcgcc ggtgcgagca gaccggaact gatccatcag
     1021 ttggtacgcc gcgtcggggt attctgcgtc ccaggttgcc tcaagccacg cccgctccgt
     1081 cgcaagccag gggcggccga tcgagagtgg atccccggta atggggcgat acgagatgcc
     1141 agggccgagc ctctgcaata cggcctcacc gtcggcggag gcaacgcgca cgccgccgtc
     1201 ttcctcttct gccgcgacca gcccaaccgc ctcctcggcg agcagggctt caaggatgtc
     1261 ctgacgagtt ccaaacgctt cgggacggcg cagccggctc agcggcagac gccgctcgcg
     1321 cggctcggtg ggccgcgcgt acaccatcgc ggatccgttg cccgccacca tgactgcagc
     1381 gcgagggttt cggcgccaga tcaccggatg cgccagcacc ggtacactcc gatcccggaa
     1441 ccaatcggcc aggtcgaggt gggtgtgcac agctgctgag ccgtggtcgc tcaccaggag
     1501 gatcaacgaa cgctcgagct caccgcgtgc tcgcagcgtc tggaccatgc ggcccaccac
     1561 gtcgtccagc ttgcgcagcg accgtcgtac taacggtccc atcggcccgt cctgatgggc
     1621 atatccgtcg acggcgggga attgcgcaaa tacgaaacgc gctagccccc cggctgcgtc
     1681 aatgagatac cgagcgacgg cgtcgtccga cggctgatgc cattcggcgt agtgcgcaag
     1741 tattccccag aacttgcgtg aggcgcgggt cggatcgcga tccgatgaga gaccacgggc
     1801 cactggtgtg aagagtccca gactgtgcgg gaccagctcg aagatggtgc gaacgttggg
     1861 agcaatgtcg gcatcgagca ttcccgcctg atagccgcag tagcttcgca ccgcgttgcg
     1921 gtcacgccac cagcgaccag cgtagtgccg gcggtcgagc caccggatcg agggcacgtc
     1981 gcagcgcccc ggcatgcatc cggtgaggaa gggcaggtat gcgacgctcg tcgtcgaagg
     2041 aaaggccgta atcgcgcagg aacgcccgcc gtcttccacc atggcggcga ggttggggag
     2101 gtttccggcg gcaagctcgg tctcagccag gtcgcggcgc aggccgtcga cgagcaggag
     2161 aaagcaacgg gtcatgtcac ctcgaatgta tatcgcgatt gtgcgcccgc gtcatcatgt
     2221 atcggtttgg cacacgctca cggtgcgacc caccgcgcgg cgaccaagag agacaatcca
     2281 ccgaccgcgc cccagaggag cacgccctgc aggaacggcc gcggcccgag ccgcttcagc
     2341 gtactccgag tcagtgtcgc accgatcaga aagagcgtca gcaccagggc aatccgtgct
     2401 gcatcggcga tatcgtccag caccggagct gtgctggggg gcaacaccga gcgagaaaag
     2461 gccgcgagcg cgaacaaacc gatgaaccag ggtagcggca ctcgagtggt ggtcgcgctc
     2521 ccgctccggc gggccacgat ggcagcggcc acggtgagcg gcacgatcca gagcgcacgc
     2581 gccagcttca gtaccgtcgc ctgctccagc gccaacggtc cgtaactgga cgctgcgccg
     2641 accacggaac tggtgtcgtg gatggcgacg gcggcccaga tggcgaactg gtgctggctc
     2701 aggtgcagca tccggccgac cggcggaaag agatagaggg ccacagcgtt caggagaaag
     2761 atcgtcgcca gtgaaacgct cattgcctcc ggtcgcgcgc cgatggccgg acccaccgcc
     2821 gcgatcgcac tgccgccgca gatgctggtg cccgctgtaa taaggaaaga cgtcttctgt
     2881 tcgatccgca gccaggagcc gatcagaagg ccaaggacga gcgcggtggc gatgcctgcg
     2941 acggtatagc cgactcccat ctgccctgcc gcgagcacgg cgctgaacgg catgccgaag
     3001 cccagtccca ctacggcggt ctggagcagc cagcgggcgg ctcgcccggt caaccggggc
     3061 actggattcc cgacggtcaa tgccagcgcg gctcctagtg cgagcccgat cgccggcgtc
     3121 gcccaggggg tgagcgaaaa cgcggcagcg agcccgaaga ggatgaggct gaagcgcccg
     3181 gctgaagtgc cttctcgagt cacggtgctc ctgaccttac ggcgctcctg agcttaacaa
     3241 gcgtcggcgc tcggcgagcg agcgaagccc acggggagat ccgcacgaaa gagcccacgg
     3301 cggaaacacc gtgggccttg atgtctcagt cggggcgccc ggatttgaac cggggacctc
     3361 ctgctcccga agcaggcgcg ctaaccgggc tgcgccacgc cccgatacca catgcgctca
     3421 gagggactcg aacccccagc cttctgatcc gtagtcagat gctctatcca attgagctaa
     3481 gggcgcggct cgatgggcgg tacaagactc gaacttgtga cctccacgat gtcaacgtgg
//
LOCUS       DASRJQ010000180         4361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2697971, whole genome shotgun sequence.
ACCESSION   DASRJQ010000180 DASRJQ010000000
VERSION     DASRJQ010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2697971"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VFB89_04050"
     CDS             <1..580
                     /locus_tag="VFB89_04050"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZM26501.1"
                     /translation="SAGQARPLASCRVGDARALDFRDHSADILLLLGPLYHLTEAADR
                     ARALDEACRVLKPGGQLFAAAISRWASALDGLSRDLFRDPRFTAIVERDLQEGQHRNP
                     TERLDYFTTAYFHRPDELRAEVLAADLAIDGVYGIEGPGWILSDLAQRLEDPRRCADL
                     LHVARQFESDPAVLGVSAHLMVVARRPASDLS"
     gene            626..1219
                     /locus_tag="VFB89_04055"
     CDS             626..1219
                     /locus_tag="VFB89_04055"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HZM26502.1"
                     /translation="MPSKAAYVLVVPGFADWEPAHALAELRRHGGYRVEVVGLTMAPV
                     ESMGGLTVQPKRTLADVDLTDVAAFILPGGDRWESSPMEPELAEFLHRLEAAHIPLAA
                     ICAATVAITRAGLLHGRRHTSNGQNYLRHHVPGYEGAGDYVDAPAVRDRGLITASGLA
                     DVEFAAELLSELDVLSEKERRLWVELFRGGRIPARVA"
     gene            1221..1751
                     /locus_tag="VFB89_04060"
     CDS             1221..1751
                     /locus_tag="VFB89_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZM26503.1"
                     /translation="MGSPGAGGSQANSEAANTRLLAALLDSWDRNNTILVNLLRALPD
                     GALDLRPTASSPSIGGLFAHIHYVRLVFVAEDVPECNTPLPEGEWRMERDRDRIAQRL
                     QDSARTVREAVASRLQAGRAMTVHYDHPLLFLQHMIWHEGYHHGQIKLALKLSGQALA
                     DEDIGPGTWDVWMDKT"
     gene            1798..2340
                     /locus_tag="VFB89_04065"
     CDS             1798..2340
                     /locus_tag="VFB89_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26504.1"
                     /translation="MVQTEQREIHGLPVRIVNTRPDIQTEQVARRLAAALDLVATYAP
                     RCYRRLRTDLAGFVVERFACRGAFFPMSRECLVELTFTVNPEVSLPEIAASIVHEATH
                     ARVAHWCKSPPPEHRGREERLCRRAELDFGLALPDGDRVVQRAQASLALTEQEVAPTI
                     DWQEAARRVAEVDAAARRTL"
     gene            2356..2808
                     /locus_tag="VFB89_04070"
     CDS             2356..2808
                     /locus_tag="VFB89_04070"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZM26505.1"
                     /translation="MVVNRSAPSATVVPVLVYEDVAKAIDWLCDTFGFTERLRAGPPS
                     GSVTHAQLDIAEGAVMLGRQGGEFRPPQPGGVSQYVAVHIQDVDTHCEHARQCGARIL
                     KAPTNMPFGERQYTVEDLGGHRWTFSQSVADVPLADWGAASPKPREPA"
     gene            2840..3310
                     /locus_tag="VFB89_04075"
     CDS             2840..3310
                     /locus_tag="VFB89_04075"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HZM26506.1"
                     /translation="MSDDRLEHLRRFSYWLDEGIRIPGTRLRIGLDPILGLVPGVGDA
                     LGAVLAAAILVEAVRRRVTRFTLVRMAGNIALDAVVGSVPLIGDVFDAGWKANQRNIA
                     LLERHIAGPLEARKTDRLFVVALTGVLLVLCAALMVGGALLAAGILRSLLGGPR"
     gene            complement(3318..>4361)
                     /locus_tag="VFB89_04080"
     CDS             complement(3318..>4361)
                     /locus_tag="VFB89_04080"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZM26507.1"
                     /translation="DYDGGYMPGWPGMERFKGQTIHPQKWPEDLDYAGKRVLVIGSGA
                     TAVTLVPAMVEGAEAASHVVMLQRSPSYVASLPTRDKIANAMRRFLPERLAYAGARWK
                     NVLRGMLYYALARGRPAVFQGMLRKGASRALGPDYDVDAHFTPRYAPWDQRLCIAPDA
                     DLFRAIRKGRASMVTDAIASFTEDGILLGSGAHLDADIVVAATGLTMKLFAGLTLVVD
                     GQTVDLSRTMAYKGMMFSGIPNLASALGYTNASWTLKCDLVAEHVCRLLNYMTRRGFV
                     QATPRRDPGVEEEPVLSFSSGYVQRALPSLPKQGSRSPWRLHQNYVKDLFALRMGRVD
                     DPALEFRAPAQGA"
BASE COUNT          713 a         1442 c         1467 g          739 t
ORIGIN      
        1 aagcgccggc caggcccggc cccttgcttc gtgtcgagtc ggtgatgccc gagccctgga
       61 cttccgcgac catagcgccg acatcctcct cctgctgggc ccgctctatc acctgactga
      121 agccgcggat cgtgcgcgcg ccctcgacga ggcgtgtcgg gtgctcaaac cagggggaca
      181 gctctttgcc gccgccatct cgcgctgggc ctcggcgctg gatgggctgt cccgcgactt
      241 gtttcgcgat ccccggttta cggccatcgt tgaacgagac ctgcaggagg ggcaacatcg
      301 gaacccgacc gagcggctcg actacttcac cacggcgtac tttcatcgcc cggacgagtt
      361 acgagccgag gtgctggccg cggacctggc cattgacgga gtctacggca tcgaggggcc
      421 gggatggatc ctttcggatc tcgcgcagcg cctcgaggat ccgcgccgct gcgctgatct
      481 cctccacgta gcgcggcagt tcgagtccga cccggcagtg ctcggcgtaa gcgcgcacct
      541 gatggtggtc gcccggaggc cagcgtcgga tttatcttga agacgaagcc gccgtccgct
      601 gggatcgtct gcaggaggag catcagtgcc ttcaaaagcc gcttatgtcc tggttgttcc
      661 cgggttcgca gactgggagc ccgcccatgc actcgccgag ctgcgccgtc acggaggcta
      721 ccgcgtcgaa gtcgttgggc tcaccatggc ccccgtggaa tccatgggtg ggctcacggt
      781 acagcccaag cgcacgctgg ctgacgttga tctcaccgat gtggccgcat tcatcctgcc
      841 gggcggcgac cgctgggaga gctcgccgat ggaaccggag ctcgccgagt ttttgcaccg
      901 gctggaggct gcgcatatcc cgttggcggc aatctgcgct gcgactgtcg ccatcacgcg
      961 agccggcctc ctgcacggcc ggcgtcacac gagcaatggg cagaactacc tgagacacca
     1021 cgtccccggc tatgagggag cgggggatta cgtcgatgca cccgcggtcc gggatcgcgg
     1081 actcattacc gcgagcggac tcgcggatgt ggagttcgcc gcagagctgc tgagcgagct
     1141 cgacgtgctc agcgagaagg agcgccggct ctgggtggag ctctttcgag gcggacgcat
     1201 cccggcccgg gtcgcctgac gtgggctctc caggagccgg agggtcgcag gcgaactcgg
     1261 aggcagcaaa cactcgactg ctggcagccc tgttggattc atgggaccgg aacaacacga
     1321 tcctcgtcaa cctgcttcgt gcgctgccgg acggggcgct cgacctgcga cccacggcga
     1381 gcagcccttc gatcggcgga ctgttcgccc acatccacta tgtccgcctc gtcttcgtcg
     1441 ccgaggatgt ccccgagtgc aatacgcccc tgcccgaggg agagtggcgg atggagcgtg
     1501 accgcgatcg gatcgcgcag cggctccagg acagcgcacg cacggtacga gaggcggttg
     1561 cgagccggct ccaggcaggt cgagcaatga ccgtgcacta tgaccatccc ctgctcttcc
     1621 tgcagcacat gatctggcac gagggctacc atcacgggca gatcaagttg gcgctcaagt
     1681 tgtccggcca ggctctggcc gacgaagaca tcgggcccgg cacgtgggac gtctggatgg
     1741 acaagacgtg agacctccgc cgtcaactgt acctggtaga accactgggg tgaaatgatg
     1801 gtacagaccg agcagcgcga gattcacggc ctcccggtac ggatcgtgaa cacccgaccc
     1861 gacatccaaa ccgaacaagt cgcccggcgc ctggccgcgg ccctcgatct cgtcgccacc
     1921 tatgcgcccc gatgctaccg acggctccgc accgacctcg ccggcttcgt cgtggagcgc
     1981 ttcgcctgcc gaggggcctt ctttccgatg tcgcgggagt gcctggtcga gctcaccttc
     2041 acggtaaatc cggaagtctc gctgcctgaa attgccgcct ccatcgtgca tgaggctacc
     2101 catgcccgag tggcgcactg gtgcaagtct cccccgcctg agcatcgggg acgcgaggaa
     2161 cggctgtgcc gccgggccga gctcgacttc gggctcgccc tgcctgatgg cgacagggtt
     2221 gtacagcgcg cccaggcctc cctggcgttg accgaacagg aagtcgcccc cacgatcgat
     2281 tggcaggaag cggctcggcg ggtcgccgag gtcgacgccg cggcgcggcg cacgctctag
     2341 aatgcggagg tgagtatggt cgtcaaccgg tcagccccga gtgcaaccgt cgtacccgtg
     2401 ctggtctatg aggacgtcgc caaggcgatc gattggctct gcgacacctt cggtttcacg
     2461 gagcgtttga gggctggtcc gccgagcggc agcgtcaccc acgcccagct cgatattgcc
     2521 gagggcgcgg tcatgctggg ccgccagggt ggcgaattcc gtccgccgca acccggcggc
     2581 gtaagccaat acgtggccgt gcatatccag gacgtcgaca cgcattgcga gcatgcccgg
     2641 cagtgcgggg cgcgcattct caaggcaccg acgaacatgc ccttcggtga acggcaatac
     2701 accgtcgagg acctcggcgg gcaccgctgg acattctccc agtcggtcgc cgatgttcct
     2761 ctcgcagact ggggtgcggc ttcaccgaag ccacgtgagc cggcataacc cgcgcttgct
     2821 ccgaacgaaa gcgcgggacg tgtcggacga tcggctcgaa cacctccggc gattttccta
     2881 ttggctggat gaggggattc ggattccggg gacccggctg cgaatcggcc tggacccgat
     2941 cctggggctg gtacccggtg tcggagatgc tctgggcgcc gtgctggctg ccgcgattct
     3001 agtggaagcg gttcgccgga gggtcactcg gttcacgctc gtccggatgg ccggcaacat
     3061 cgccctcgac gcagtcgtgg gttccgtccc actcatcggt gacgtcttcg atgccgggtg
     3121 gaaagccaat cagcgaaaca tcgccctcct ggagcggcac attgccgggc cgctggaagc
     3181 gcgaaagacg gatcgactct tcgtggtcgc actgaccggc gtgctcctgg tgctctgcgc
     3241 ggcactgatg gtcggcggtg cgctcctcgc cgcggggatc ctgcggagct tgttgggggg
     3301 gccgcgctag cgcatcccta cgccccctgc gctggagcgc ggaactcgag cgccgggtcg
     3361 tccacccgcc ccatacggag ggcgaagagg tccttcacat aattctgatg caggcgccag
     3421 ggtgaccgcg agccctgctt gggaagcgac ggcagcgcgc gctggacgta tcccgaggag
     3481 aagctgagga ccggttcctc ctctaccccc gggtcgcggc ggggcgtcgc ctgcacgaac
     3541 ccgcgtcgcg tcatgtagtt cagcagccgg cagacgtgct cggcgacgag gtcgcacttc
     3601 agggtccatg aggcgttggt gtagccgagc gcagacgcca gattcgggat gccgctgaac
     3661 atcatcccct tgtaggccat ggtgcgcgac aagtccaccg tctggccgtc gacgacgagg
     3721 gtcaggccgg cgaacagctt catggtgagg ccggtggccg caacgacgat gtccgcgtcg
     3781 agatgtgcgc cggaacccag caggatcccg tcctcggtga acgacgcgat ggcatcggtg
     3841 accatcgatg cgcgtccttt gcgaatggcg cgaaacaggt cggcgtcggg cgcgatgcag
     3901 agccgctggt cccacggcgc gtaccgcggc gtgaaatggg catcgacgtc gtagtccggt
     3961 cccagcgccc gcgaggcccc tttgcggagc atgccctgga agaccgccgg gcgcccgcgc
     4021 gccagcgcat agtacagcat gccgcggagc acgttcttcc agcgcgcccc cgcgtaggcc
     4081 agtcgctccg gcagaaaccg gcgcatcgcg ttcgcgatct tgtcgcgggt gggcaacgag
     4141 gcgacgtagc tgggtgagcg ctggagcatg acgacgtgcg aagcggcttc cgcgccttcg
     4201 accatggcgg gcaccagcgt cacggcggtg gcgccgctgc cgatcaccaa aacgcgcttg
     4261 ccggcgtagt cgaggtcttc gggccatttc tgcggatgga tggtctgacc cttgaagcgc
     4321 tccatgcccg gccagcccgg catgtagccg ccgtcgtagt c
//
LOCUS       DASRJQ010000181         2949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3822732, whole genome shotgun sequence.
ACCESSION   DASRJQ010000181 DASRJQ010000000
VERSION     DASRJQ010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3822732"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VFB89_04085"
     CDS             complement(<1..277)
                     /locus_tag="VFB89_04085"
                     /inference="COORDINATES: protein motif:HMM:NF020250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HZM26508.1"
                     /translation="MARRSRPFVLLALAVLSGGAAAYLALQYIRQQATPLLAATPKNS
                     QVVVAAHAMPLGAVLGERDVKTVEWAGGALPPGFIGSTAEAVGRGLIV"
     gene            complement(287..706)
                     /locus_tag="VFB89_04090"
     CDS             complement(287..706)
                     /locus_tag="VFB89_04090"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HZM26509.1"
                     /translation="MRRLRSLSRQDRGQALLEFALILPLLLLLVLGIIEFGRAWNLAQ
                     LMSDVAREGARRAVVADPTITETQVHDFMDQKLATAGVPIGSATITFTKTGANPTTGW
                     HSAGQQTVTVVIPYSFMFFGDVFGTIPLTSSFTMRME"
     gene            complement(703..1242)
                     /locus_tag="VFB89_04095"
     CDS             complement(703..1242)
                     /locus_tag="VFB89_04095"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HZM26510.1"
                     /translation="MHEPYLQHGPWLSVGLVAVLVVACWTDWRSNRIPNWLTLAGMVL
                     GLGLRLAEGPSALVEGSLGFGLAVLVALPAFATGVLGGGDAKLLMAVGAVLGPRNFGF
                     AVLAIAVAGGLLALAEALRRGMLRPALALCGATLLHWVTFGRLGPVPRPLAAQKVTVP
                     YGLAIAAGTMVWWFLGGVS"
     gene            complement(1317..1508)
                     /locus_tag="VFB89_04100"
     CDS             complement(1317..1508)
                     /locus_tag="VFB89_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HZM26511.1"
                     /translation="MQSMLRALWADESGQGLVEYALIIALVAVGLIAILLVLRNSIGN
                     VFKNAASQLNNAPSAPFTP"
     gene            2123..>2949
                     /locus_tag="VFB89_04105"
     CDS             2123..>2949
                     /locus_tag="VFB89_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26512.1"
                     /translation="MSVPDPSVSVTAPPMRDRIATRSARPSLYDIIFIFWAIAIPLGF
                     SNRLLSSDGDLARHLRLGEVMLDQHAVLRREFFSHTAAGRPFLAFEWLSEVVYAAAER
                     IGGLALVAIVASLLLALTFTLLARFLIRRGADPLLAYLVSMAAAVLTGSHWLARPHLF
                     TLLAVVILLDLLERTEPPPVWLFAVLFAVWANFHGGFVYGLILIGAYLVGDLLEARSA
                     DDPALWKRRARARGTAFGVALLATLVNPYGIKLLMHVSGFFGNDAIIRQTNEFQSPD"
BASE COUNT          553 a          974 c          895 g          527 t
ORIGIN      
        1 gtacgatgag cccccggccc accgcctctg ccgtgctacc gatgaatcct ggaggtaggg
       61 cgccgccggc ccattccacc gtcttgacgt cccgctcacc gagcacggcc ccgagaggca
      121 tggcatgcgc ggccactacc acctgagaat tcttgggcgt cgcggcgagt agcggcgtcg
      181 cctgctggcg gatgtactgc agggccagat acgccgcagc gccgccggac agtacagcga
      241 gcgccaacaa gacgaacgga cggctcctgc gcgccatatg ctctcctcac tccatccgca
      301 tcgtgaatga gctcgtcagc gggatcgtgc cgaagacgtc gccaaagaac atgaacgagt
      361 agggaatcac taccgtcacg gtttgctggc ctgccgaatg ccaccccgtc gtgggattgg
      421 caccagtctt ggtgaatgtg atggtcgccg atccgattgg aacgcctgcg gtagcgagct
      481 tctggtccat gaagtcgtgg acctgggttt cggtgatggt cggatcagcg acgacggcac
      541 gtcgcgcgcc ttcccgggca acgtcggaca tgagctgggc caggttccag gcccggccga
      601 actcgatgat gccgagcacg agcaacagca agagcggcag gatcagcgcg aattcgagca
      661 gtgcttgccc gcggtcctgg cgggagagcg aacggagccg tctcacgaca cacctccgag
      721 gaaccaccac accatcgtgc ccgcggcgat agccagcccg taaggcaccg taaccttttg
      781 cgcggccagg ggccggggga ccggccccaa tcgaccgaag gtcacccagt gaagcaacgt
      841 ggcaccgcaa agcgccagcg cggggcgcag catcccgcgg cgaagcgctt ccgccagcgc
      901 cagcagcccg ccggcaaccg cgatagccag cacggcgaag ccgaagttgc gagggccgag
      961 gacggcaccc acggccatga gcaacttcgc gtcgcctccg ccaagcacgc cggtcgcaaa
     1021 cgccggcaac gcgaccagca cggccagccc gaagcccaga ctaccttcga cgagtgcgga
     1081 cgggccttcg gcaagtcgca ggccgagtcc gagaaccatg cctgcgagcg tcagccaatt
     1141 cgggatccga tttgaccgcc agtccgtcca gcacgcaacc accaagacgg ccaccaggcc
     1201 gacactcagc caggggccgt gttgaagata cggttcatgc atgctgcact tttgtccccg
     1261 gctgggcaag agtggaactc tcacccactc ttgcccgcca acggccgggt ccggcttcag
     1321 ggcgtgaacg gcgcagacgg cgcgttgttc agctgcgagg ccgcgttctt gaatacgtta
     1381 ccgatcgagt tgcggagcac cagcagaatc gcgatgagac cgaccgcgac cagagcgatg
     1441 ataagcgcgt attccacaag gccctgaccg gactcgtcgg cccacagcgc tctcaacatg
     1501 gactgcatgc agctcctcct cggcctcccg gccagggtgg tggtgtatgg gcccccgcgg
     1561 aaagagacag gggtcctcgc cgacaggccg gtcggcccgt caggggcgat cggtactcgt
     1621 ccctcgttca aatgcttgct gacagaattg acttaatatt gaacgctgca gaaactagca
     1681 agacgtatgc cctgcatcac ggtcagggtg ggtccgatta ggtaacccat tggtgcaatg
     1741 gcacttgcca aacctgctga ctcacttcta ttccgaagta gtcactgtac ctcggctcgc
     1801 tcattggcgg catagtgaca gtgaacaaaa tgtcgccagt aacgcaacac cataagcggc
     1861 aacgtgttgg gtaaaccgct cgtgccaaca gtccggatat tactcgcttg cggcgagcgc
     1921 tattcgacac caagccgcct ctgtggttgc tgcggagcat caacaagtga tgttcgagag
     1981 ggacaagacg agtggacttt gagccgcagc ttccggcccg gatgatgctc ccccctcctc
     2041 ggtcggttaa gaaccctcga cctctcgccc cccgcaagga ttcgtgcgaa atgttctttg
     2101 gctccccgga gcccacagct cgatgagtgt gccggatcca tcagtgtccg taacggcacc
     2161 gccgatgcgc gatcgcatcg ccacgcggag cgcgcggcca tcgctgtacg acatcatctt
     2221 catcttctgg gcgatcgcga tcccgttggg cttttccaac cgcctgctga gcagcgacgg
     2281 tgatctcgct cggcacctcc gcctaggcga agtgatgctg gaccagcacg ccgtgctgcg
     2341 ccgggagttc ttctcccaca ccgccgcggg ccgtcctttc ctggcgttcg agtggttgag
     2401 cgaggtggtc tatgcagccg ccgagcgaat cggcggtctc gccctcgtag ccatcgtagc
     2461 cagtctattg ctcgcgctga ccttcacgct gctcgcgcgg ttcctgatca ggcgcggagc
     2521 ggatccattg ctcgcgtatc tggtgagtat ggccgccgcg gtgctcaccg ggagccattg
     2581 gctggcgcgc cctcacctct tcaccctgct cgcggtggtc atcctcctcg acctcctcga
     2641 gcgcaccgag ccgcctccag tgtggctatt cgcagtgctg ttcgctgtat gggccaattt
     2701 tcacggcgga ttcgtctacg gcttgatcct gatcggcgcc tatctggtcg gcgatcttct
     2761 cgaggctcgg tccgctgatg atcccgcgct ctggaagcgc cgggcccgcg cacgcggcac
     2821 ggcgttcgga gtagcgttgt tagcaaccct ggtaaacccg tacggcatca agctgctgat
     2881 gcacgtgagc gggtttttcg ggaacgatgc cattatccgc caaaccaatg aattccagtc
     2941 gcccgactt
//
LOCUS       DASRJQ010000182         2493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1007668, whole genome shotgun sequence.
ACCESSION   DASRJQ010000182 DASRJQ010000000
VERSION     DASRJQ010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007668"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VFB89_04110"
     CDS             complement(<1..891)
                     /locus_tag="VFB89_04110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZM26513.1"
                     /translation="MSASSALPESGSLTPEEAEAVLRELAAVFGNGGASPETKSNRGS
                     RPDLEAMYRTLVEQIPAVVFMAYLDRGIGEAYVSPRIEEALGFTQAEWLEDPVRWYNR
                     IHPDDQHRWSIEAAETFATGKPLRSAYRVIARDGRVIWFHCEAKMIRQEDGRPWFIHG
                     IGFDISDLKRAEQALEGERNLLSTVLDTVGALVVVLSPAGAILRFNRACEDISGYTFD
                     EVRGKYFGDLFLTPEEAARFKSNLATLHAKKPVTGSESDLITRNGGRRRISWSSTVLP
                     GVGIAPTYVIATGIDITERKR"
     gene            complement(900..1895)
                     /locus_tag="VFB89_04115"
     CDS             complement(900..1895)
                     /locus_tag="VFB89_04115"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26514.1"
                     /translation="MPISEISILWLSAGLSCDGDSIAMTAATQPSIEDLLSGAIPWTP
                     RVKLYNAFLAPEVGDEFVRFFYQGAAGELGPFILVVEGSIPDETNKPEGYWATFGTDP
                     KTGQPITTCAWIDRLAPKAWAVVAAGTCAAYGGIHAMAGNPTGCMGLPDYLGWNWRSQ
                     AGIPLVCVPGCPVQPDNLMETLLYLLYQAAGRSPPIPLDDSLRPTWLFTQTVHEGCDR
                     GGYYEQGEFAEEYGSRLCIVKLGCWGPVVQCNVGKRGWMGGIGGCPNVGGICIGCTMP
                     GFPDKFQPFMDQPPGSLLSSAAVMTYGKAIQALRRFTQASLNKAPSWRIQRKQSD"
     gene            1944..2324
                     /locus_tag="VFB89_04120"
     CDS             1944..2324
                     /locus_tag="VFB89_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26515.1"
                     /translation="MSPRSERATFFKSISGSAFMSQAERRSSQRVPYRTDVRPLLVVE
                     DRAYEIIDVGERGIRARCADTDSWLHGSVVHGSVWFQRDRKVPIEGLVVRAGGGELAL
                     KLAGDGIPVATLIDEARYARISLG"
BASE COUNT          424 a          812 c          808 g          449 t
ORIGIN      
        1 gcgcttgcgc tcggtgatgt cgatgccggt tgcgatcacg tacgtcggtg cgattccgac
       61 accgggcagg accgtgctcg accaggaaat gcggcgccgg ccgccattgc gagtgatcag
      121 atcgctttcg gatccggtga cgggcttctt cgcgtggagt gtcgcgagat tgctcttgaa
      181 gcgagccgcc tcctccggtg tcaggaacag atcaccgaag tattttccgc gcacctcatc
      241 gaacgtgtag ccgctgatgt cctcgcacgc gcggttgaac cgcaggatgg cgccggccgg
      301 actcagcacg acgaccaacg ctcctacggt gtcgagcacc gtcgacagca ggtttcgttc
      361 tccctccagc gcctgctcgg cgcgtttcaa gtccgagatg tcgaagccga ttccgtggat
      421 gaaccaggga cgtccgtcct cctgccgtat cattttggct tcgcagtgga accagatcac
      481 ccgcccatcg cgggcgatga cgcgataggc cgaacgaagc ggcttgccgg tggcaaacgt
      541 ctccgcggcc tcgatgctcc agcgatgctg gtcgtcggga tgaatccggt tgtaccagcg
      601 caccggatcc tccagccact ccgcctgggt gaagccgagc gcctcctcga tgcgcgggct
      661 gacgtaggct tcgccgatgc cgcggtccag gtacgccatg aacaccaccg ctggaatctg
      721 ctccaccagc gtccggtaca tggcttcgag atcgggccgc gatcctcggt tgctcttcgt
      781 ctccggactc gcgccgccgt tgccgaacac cgcggcgagc tcgcgaagaa cggcctccgc
      841 ctcctcgggc gtcagcgaac cggattccgg aagtgccgag gatgcggaca tgcgggagct
      901 cagtctgatt gcttccgctg gatgcgccag ctgggagcct tgttgagcga tgcctgagtg
      961 aatcgcctca gtgcctggat cgccttaccg taggtcatca ccgcggcaga ggagagcagg
     1021 gagcccggcg gctgatccat gaacggctga aacttgtcgg gaaagccggg catcgtgcag
     1081 ccgatgcaga tacccccaac gttgggacat ccgccgatgc cgcccatcca gccccgcttg
     1141 cccacattgc attgaaccac cggcccccag caccccagct tgacgatgca caggcgcgat
     1201 ccgtactcct cggcaaactc cccctgctcg tagtacccgc cgcggtcgca gccctcgtgg
     1261 accgtctggg tgaacagcca ggtgggccta agcgagtcgt ccagcgggat ggggggcgac
     1321 cggccggctg cctgatatag aaggtatagg agcgtttcca tcaggttgtc gggttgcacc
     1381 ggacagccgg ggacgcagac gagcggaata ccggcctggg agcgccagtt ccagccgagg
     1441 taatcgggca gacccatgca gccggtggga ttgccagcca tggcgtggat tccgccatac
     1501 gccgcgcagg ttccggcggc cacgaccgcc caggccttcg gtgccagccg atcgatccag
     1561 gcgcacgttg tgatgggctg cccggttttg ggatcggtgc cgaaggtggc ccagtagcct
     1621 tcgggcttgt tggtctcgtc gggaatcgat ccttccacca cgaggatgaa aggccctaac
     1681 tcaccggccg cgccctggta gaagaagcgg acgaactcgt cgcctacttc cggcgccagg
     1741 aaggcgttgt agagcttgac cctgggggtc cagggaatcg cgccgctcag cagatcctcg
     1801 atgctcggct gggtggcagc cgtcatggcg atcgagtcgc cgtcgcagct gagtcccgcg
     1861 ctgagccaga gaatggagat ttcggagatc ggcacgccct gcatgctcta ccgtaacact
     1921 gcccggcagg ggtcgccagc gacttgtcac cgcgcagcga acgggcgacc ttcttcaagt
     1981 ccatctccgg cagcgcattc atgagtcagg ccgagcgccg ttcctcacaa cgggttccct
     2041 atcggacgga tgttcgcccg ctactcgtcg tcgaggacag agcgtacgag atcatcgacg
     2101 taggcgagcg cgggattcgg gcccgctgcg ctgacactga tagttggttg cacggatctg
     2161 tcgtgcatgg gagcgtgtgg ttccagcgcg accgcaaggt tcctattgaa gggctcgtgg
     2221 tgcgggcggg tggaggcgag ctggcgctca agctggccgg cgatggcatt cccgtcgcca
     2281 ccctgatcga cgaggcgcgt tacgcccgta tctcgctggg ctgagccgtg ccgcgctccc
     2341 tgatccacga ttggaacggc gagcgcgtgc tcagcccggg gcgccgagtc gagctgaacg
     2401 acgagacgct gcgcgacggc ctccagagtc cctcggtcac cgatcccccg ctcgaagcca
     2461 agctccgtct actccatctc atggccgacc tcg
//
LOCUS       DASRJQ010000183         9554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_442604, whole genome shotgun sequence.
ACCESSION   DASRJQ010000183 DASRJQ010000000
VERSION     DASRJQ010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 9554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9554
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442604"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..715)
                     /locus_tag="VFB89_04125"
     CDS             complement(50..715)
                     /locus_tag="VFB89_04125"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HZM26516.1"
                     /translation="MKLTIFAATGGIGRQLLDQAIAAGHDVTAVVRNLVNLPSGIRIV
                     TADLATANRATLESAVRGADAVLSGLGPRALTKAGIAAEGTRAIVEAMKAADARRLVV
                     VSAAPIGTVPSPNRPNPPKHDPGDGFFMRHLGAPFARTVMRERYADLALMEDILSYSG
                     LDWTIVRPPRLTNGPLTRRYRTALGQNLRRGVVISRADVAHFMLRVLEQPETIGKVIG
                     IAY"
     gene            complement(787..1728)
                     /locus_tag="VFB89_04130"
     CDS             complement(787..1728)
                     /locus_tag="VFB89_04130"
                     /inference="COORDINATES: protein motif:HMM:NF007214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HZM26517.1"
                     /translation="METRAAAFTVVRPQLFAIAYRMLGSVVEAEDLVQEAYLRWQEAS
                     DVEVRSPSAYLTTIVTRLAINQLRSARAQREVYVGPWLPEPLVTTSGSDAPDTVELAE
                     SLSMAFLVLLERLSPIERAVFLLHEVFDFDYAEIARVVEKTEANCRQILTRARKHLGA
                     SRQRFEAAPAQADRLVQRFTRASQAGDLDGLLEVLAQDITLWADGGGKVPGAALKPVH
                     GARAVARFAIGVVHRFVPADSVIRAAQINGEPGFIVYVSGKPLAALVFQIRGDRIHTI
                     YAIGNPEKLSAISYQLSEVHFDFRSGAPLVNRLIADS"
     gene            1831..2061
                     /locus_tag="VFB89_04135"
     CDS             1831..2061
                     /locus_tag="VFB89_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26518.1"
                     /translation="MDVVSYRGFAITARTFQIRGSGRWTRDLLIGRRQELRAFSDPRT
                     YPTKKSAMAGCWDLGRRIIDGRVRNCSVDDLS"
     gene            2136..2387
                     /locus_tag="VFB89_04140"
     CDS             2136..2387
                     /locus_tag="VFB89_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26519.1"
                     /translation="MIARCDTLTQALDELEGGVLAGASTIVVSRGSWDALSSNERDAY
                     RIRANRIGIELWVDDAISSHFVEVRGGEDAPPLSTERHV"
     gene            complement(2435..2710)
                     /locus_tag="VFB89_04145"
     CDS             complement(2435..2710)
                     /locus_tag="VFB89_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26520.1"
                     /translation="MPSHLARIERSNGRVLDNVEVLLTVSELRPGRPAWSGEFLSRSG
                     DGFQPSERFGLTLDNGQRGTASVSRTHLDSRTPEQTRIEFIGSGPLI"
     gene            complement(2734..3663)
                     /locus_tag="VFB89_04150"
     CDS             complement(2734..3663)
                     /locus_tag="VFB89_04150"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZM26521.1"
                     /translation="MVKFLYIYMGEHHVMDVSFVIRRRLEEFGLDQRDLARAAQVTES
                     YISQLLTRKKAPPAPNRTDIYEKMDKFLKLPEGELARLATLQRRELVKRELGDEPAPL
                     FEEVRTLILRKCEPDREKQMRAVFEKEPFGEIERLVTQKLLDVVKRVAKDELANEYWL
                     RMVAELGGRSYEEMRVAVLQFLDADILDVSVEDCVSFLDPLIETWDFDLATFELEIML
                     NHRVAAGQVKRFVFVESDSDPTGADEAGLREFLRDASLSGTATDEEIEFLKRFKFKGR
                     RPTALYYYRELQNFRDPLHFQQPPSSTEHSAPS"
     gene            3802..3978
                     /locus_tag="VFB89_04155"
     CDS             3802..3978
                     /locus_tag="VFB89_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26522.1"
                     /translation="MNDPAGQPPKGRRSSRLSPTVLLVEDGSHPHSLEKPFTRSQLGV
                     ALERCLPNAQTVEE"
     gene            4648..4896
                     /locus_tag="VFB89_04160"
     CDS             4648..4896
                     /locus_tag="VFB89_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26523.1"
                     /translation="MRILDTDNQGPVRRIHVYLTPGEAEKLTRALNRLLLDPEASEAQ
                     RVLADDSTRDLSVSIVTPAKLQDTKRYTDAERRMFAEP"
     gene            complement(4917..5792)
                     /locus_tag="VFB89_04165"
     CDS             complement(4917..5792)
                     /locus_tag="VFB89_04165"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZM26524.1"
                     /translation="MDVSLVIKQRLEQFGLDQRELARAAHVTESYISQLLTRKKAPPA
                     PNRTDIYAKMDKFLKLPQGELARLADCQRKAELKRESGDQPAPLFQEVRALVLRKCKP
                     EREPHVRAIFEQHPFGELERLITQTLLDVVKEVATRELENDYWLRMVAELGGRSYEEM
                     RVIVLEFLDTDIFHLSPDQCVSFLDPLIECWDIHLATFGLDIRLNPRVVDRPWKRFEF
                     VERGADQLFVEEPGLREFLEDPALSGSATEAEVAFLKRLRFRDKRPTPLYYYRELQSL
                     RDSLHFRPVVEKANV"
     gene            5966..6280
                     /locus_tag="VFB89_04170"
     CDS             5966..6280
                     /locus_tag="VFB89_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26525.1"
                     /translation="MYARKVSIRLKPNTSGEFTRTIDNDILPLLRKQDGFKDEITLVA
                     PDGEDAVAISLWDRKESADAYNRETYPQVLKGLARVIDGTPEVQVYEVASSTFHDTAA
                     RT"
     gene            6414..6677
                     /locus_tag="VFB89_04175"
     CDS             6414..6677
                     /locus_tag="VFB89_04175"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HZM26526.1"
                     /translation="MSILTWVLLGLIVGFVASKIVSKAGESVAINVALGVVGALLGGW
                     LFDQFGPPPVTGLSWWSLVMAATFAVFILMGYHTIAEPRGRRT"
     gene            6733..7047
                     /locus_tag="VFB89_04180"
     CDS             6733..7047
                     /locus_tag="VFB89_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HZM26527.1"
                     /translation="MSAAARAEPKVKIQPLRDRVLILPAEEAETMRGPLYIPDTAKEK
                     PTQGRVLAVGPGRIWRGERIKLDVEVGDTVIYGQYSGIPYRVGDDTLIIIKASDILAK
                     IG"
     gene            7051..8679
                     /gene="groL"
                     /locus_tag="VFB89_04185"
     CDS             7051..8679
                     /gene="groL"
                     /locus_tag="VFB89_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683025.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HZM26528.1"
                     /translation="MAAKELLFSIEARAKLKRGVDRLAEAVRVTLGPKGRNVVIDKKF
                     GSPTVTKDGVTVAKEIELADPVENLGAQMVKEVATKTSDLAGDGTTTATVLAQAIFRE
                     GVKNVTAGANPMELKRGIEKAVEVVVQELEKLSVPSAGKKEIAQVGAISANNDKEIGD
                     LIAEAMDKVGKDGVITVEEAKGIETTLETVDGTQFDRGYLSPYFVTDPEKMETVLEDG
                     YVLIHDKKISSMKELLPILEKSAESGKPLLIIAEDVEGEALATLVVNKLRGTLKVVAV
                     KAPGFGDRRKEMLRDIAVLTGGEVISEEVGFKLENTTLNQLGRAKRIVVDKDNSTIVD
                     GKGKAAAITGRINEIKVAIEKTTSDYDKEKLQERLAKLAGGVAIINVGAATETEMKEK
                     KARVEDALHATRAAVEEGIIPGGGVAFLRAQPALDRIKGTDDEKVGIAIVRRALEEPI
                     RMIAQNAGAEGSIVVAKVKASKERNFGYNAQTDAYEDLVKAGVIDPTKVARTALQNAA
                     SIAGLLLTTECVLTERREEKAAPAMPGGGMGGMY"
     gene            8771..>9554
                     /locus_tag="VFB89_04190"
     CDS             8771..>9554
                     /locus_tag="VFB89_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26529.1"
                     /translation="MPNPHVIGPLPPSTPPELRISRDQPRALPADLLRDASRRLGVMS
                     LLAATLWLVGSVADHLALRAITHGDPRWLRLDADDAIAAVSVVLSLGLFSYTRRAERD
                     PRFVLDLGLVYMVLTAAAIGLMIHWDPVPKDWHVSPMITWIGAVVLMFAAIVPSTPTK
                     TLVAALIAVSMNPLGMLIARSRGTWEFGSASNVLLMHYPDYLLAGVAAVISYVVTGLG
                     QQIARAREMGSYQLGELLGRGGMGEVYQATHRMFARPAAIKLI"
BASE COUNT         1783 a         2998 c         3066 g         1707 t
ORIGIN      
        1 agcgttgagt cgcaacgccg ccgcctactc aagagaccac tcggatgtgc tagtaggcga
       61 tcccgatgac tttcccgatc gtctccggct gctcgagcac gcgcagcatg aagtgcgcga
      121 cgtcagctcg ggagatcacg acccctcgcc gcagattttg ccccaatgcc gtccggtagc
      181 ggcgggtcag gggcccattg gtgagccgcg gcggccggac gatggtccag tccagcccgc
      241 tgtaagagag gatgtcctcc atcaaagcca ggtccgcata gcgctcgcgc atcacggttc
      301 ttgcgaacgg cgcgccaaga tgtcgcatga agaacccgtc tcccggatcg tgcttcggtg
      361 ggttcgggcg attcggcgac ggcacggtcc cgattggcgc ggcgctgacg acgacgaggc
      421 gccgagcgtc agctgctttc attgcctcga cgatggcgcg ggtcccctcc gccgcgatcc
      481 cggctttggt cagtgcccgc ggaccgaggc ccgatagcac cgcgtcggcc cctctgaccg
      541 cggactcgag cgtcgcgcgg ttcgcggtgg cgaggtcggc tgtgacgatc cgaatgccgc
      601 tcggcaggtt gaccaggttt cgcacgacgg cggtaacatc gtggcctgcc gcaatggcct
      661 ggtcgaggag ttgccggccg ataccacccg tcgctgcaaa aatcgtgagc ttcatctctg
      721 gctccttgtt cagtccagag atgatgacag gtgagcaggc cgagttgtga caggtctttg
      781 tagctatcag ctatcagcta tcagtcggtt gacgagcggt gctccgctcc tgaaatcgaa
      841 atgcacctct gatagctgat agctgatagc tgatagcttc tccggattgc cgatcgcgta
      901 gatggtgtga atcctgtcgc cccggatttg gaataccagc gcggcgagcg gcttcccgct
      961 cacgtagacg atgaacccgg gctcgccgtt gatctgggcg gcccggatca cgctgtctgc
     1021 cgggacgaac cgatggacca cgccgatagc gaaccgggcg actgctctcg cgccgtgcac
     1081 cggcttgagc gcggcgccgg ggaccttgcc gccgccatcg gcccatagcg tgatgtcctg
     1141 cgccaggacc tcgagcagcc catccaggtc gcccgcctgc gacgcccggg tgaagcgctg
     1201 caccagccga tccgcctgcg cgggcgcggc ctcgaaacgc tgccgcgagg cacccagatg
     1261 cttccgcgcg cgagtgagga tctgacggca attggcttca gtcttttcca ccacccgcgc
     1321 gatctcggcg tagtcgaagt cgaacacttc gtgcagcagg aagaccgccc gctcgattgg
     1381 cgacagccgc tccagcaaga ccaggaacgc catcgacagc gactcggcca gctcgacggt
     1441 gtccggcgcg tcggaaccgg atgtggtcac caggggctcc ggcagccagg gtcccacgta
     1501 gacctcgcgc tgagcccggg ctgagcgcag ctggttgatg gcgagccgag tgacgatcgt
     1561 cgtgaggtag gcgctgggcg agcgcacctc tacatcggac gcctcctgcc agcgcaggta
     1621 cgcctcctga accaggtctt ctgcttcgac gacgctgccg agcatgcggt aggcgatggc
     1681 gaagagctgg gggcggacga cggtgaatgc ggcggcgcga gtctccatgt cagcaatatg
     1741 acaagcgagg cggcgaagct gtgacacccg cccgcatcga ggagtcggcc ggggggctat
     1801 actacagaag tcagccggcg gaggtgcaag ttggatgttg tctcatacag gggtttcgcc
     1861 atcactgcgc gcacgtttca gatccgcggc tcgggacgct ggacccgcga tctcctgatc
     1921 ggccgccgcc aggagttgcg ggccttcagc gatccgcgaa cctaccctac caagaaatcg
     1981 gccatggcgg gttgttggga cttgggtcgc cgcatcatcg atgggcgggt gcgaaattgc
     2041 tcggtcgatg acttgtcctg actcatgact tgtcctgact cacctcgttg ttccgtgcct
     2101 ggctgccgcc gtcgcgaacc ggagtgaaaa tccagatgat tgcccgctgc gatacgctca
     2161 cgcaggcgct cgatgagctc gaaggtggag tactcgcggg cgcctcgacc atagttgtaa
     2221 gccgcggctc gtgggacgcc ctctcatcca atgaacgaga cgcctatcgc atccgggcca
     2281 atcgcatcgg gatcgagctg tgggtggacg acgcgatctc gagccatttc gtcgaagtgc
     2341 gcggaggaga ggacgcgcca ccgctttcca ctgagcgtca cgtgtaagtg ttgctcccgg
     2401 tctgcgacct ctccggtgcc tccaacccag tcggtcaaat cagtggtccc gagccaatga
     2461 actcgatgcg ggtctgctca ggcgtgcggc tatcgaggtg agtgcgactc acgctcgccg
     2521 ttcccctctg cccgttatcg agtgtcagcc caaatcgctc gctcggctga aaaccatcgc
     2581 cgctgcggga gaggaactcg ccactccatg ccggccgccc cggacggagc tcgctgacgg
     2641 tcaggaggac ttcaacgtta tccaggaccc ttccattgct gcgctcgatg cgtgccaggt
     2701 gggacggcat agcggccttc tctccttgat tggtcacgat ggcgccgaat gctccgtcga
     2761 gctcggcggc tgctggaagt ggagcggatc ccgaaaattc tgcagctccc ggtagtagta
     2821 cagcgcagta ggtcgcctgc ccttgaactt aaatcgcttg agaaactcga tctcctcgtc
     2881 ggtcgccgtg ccgctcagtg aagcatcccg caggaattct ctcagtcccg cctcgtcggc
     2941 gccggtcggg tcggagtcac tttcgacgaa cacgaatcgc ttgacctgtc ccgcggcgac
     3001 cctgtggttc agcatgatct ccagctcgaa cgtcgcgagg tcgaagtccc aggtctcgat
     3061 cagcggatcc aggaacgaca cgcagtcctc gaccgatacg tcgaggatgt ccgcatcgag
     3121 aaactggagg acggccaccc gcatctcctc gtagctccgg ccgcccagct cggccaccat
     3181 ccgaagccag tattcgttgg ccagctcgtc cttggcgact cgcttgacca catcgagcag
     3241 cttttgggtc accagacgct cgatctcccc gaacggctcc ttttcgaaca ccgcgcgcat
     3301 ctgcttctcg cggtccggtt cgcacttccg gaggatcagc gtgcgcacct cttcgaacag
     3361 cggcgcgggc tcgtccccaa gttctcgctt taccagctcc cgtcgctgaa gagtcgcgag
     3421 ccgggccagc tcgccctccg gcagcttgag gaacttgtcc atcttctcgt agatgtcggt
     3481 gcgattcggg gcgggcggcg ccttcttccg ggtaagcaac tgggagatgt aggactccgt
     3541 cacctgagcg gcgcgggcaa gatcgcgctg gtccaaccca aattcctcga gccggcggcg
     3601 gatcacgaac gagacgtcca tgacgtggtg ctcccccata tagatgtaga gaaacttaac
     3661 cataatactt aagtaccacc gctcgccacc ttcatgatcg ctgctgcact tgaccagtat
     3721 agtacatcat gggtatgttg gaacgcttga actgcagcag ttaaccgcat ctctcgccgg
     3781 gtcaagggag tcccccgcaa ggtgaacgat cccgctggcc aaccgcccaa ggggcggcgt
     3841 tcctcgcggc tctcgcccac ggtccttctg gtcgaggacg gctcacaccc tcactcgttg
     3901 gagaagccgt tcactcgcag ccaactgggc gtcgcgctcg agcggtgctt gcccaatgcg
     3961 caaacggtgg aggagtagcc ggtcgtgcca agtcagggtc aactagaacc accgacaccg
     4021 ttccgccttc gccgggtgct ggtcccttac gaccggaacc ccggtcggcc gagcaggggc
     4081 tctcgggcgg tttgggtgtg gtcgcttctc ctggtgctgg tagggctggc agtggctgtg
     4141 gccttacacg cggagttccc gttggccaac ctcacggaca agctcggcgg cttctagaaa
     4201 gcgcgagggg cgcagcgatg gcaagctggc atttcaacct tcagctggtt ccccggtcca
     4261 cctcgccggg agaccgcgac ccctggcggg caaggcagcc ggccgccagt tggcgccgcg
     4321 ccgtggatgc attcctgcct cgcgcacctt cctggagccg ccagctgagc atttggggaa
     4381 ccgacgacgg caaccggatc gacgtctggg agcacgagcg acgggtcgag tcgatcgtgg
     4441 tccggatcga cacacgtgag ccggtcgagc gcctggctcc cttctgcctg gacctggcgg
     4501 agttggccca ggctcagggc gacgtcgtct tcacctcggc ggggggccgg gtggtcgagc
     4561 ccacggccgc tgctctatcg aaggcgatct gtgcctcatc ggcctggcac ttcgtgcatg
     4621 acggggaatt cgtcggaggg ctgcccaatg cggatcctcg atacggacaa tcagggaccg
     4681 gttcgccgga tccacgtcta tctcacgccc ggagaggcgg agaagctgac gcgcgcgttg
     4741 aatcggctcc ttctcgatcc ggaggcgagt gaggcgcagc gcgtgctcgc ggacgactcg
     4801 acgcgcgacc tgtcggtctc cattgtcact cctgccaagc tccaagacac caagcgctat
     4861 accgacgcgg aacggcggat gtttgccgag ccctgagcgt gtttagtgat atttgatcag
     4921 acattggcct tctccacgac gggacgaaaa tgaagtgagt cccgcaggct ttgtaactcc
     4981 cggtagtagt agagcggcgt tggccgctta tccctgaacc tcaggcgctt gagaaacgcg
     5041 acttccgcct cggtcgcgct gccgctgagt gcgggatcct ccaggaattc cctgagcccc
     5101 ggctcttcga cgaatagctg gtcggccccc cgttcgacga actcgaatcg cttccaaggc
     5161 cgatcgacca ctcgggggtt caatcgaatg tccagcccga aggtcgcgag gtggatatcc
     5221 cagcactcga tcagcggatc caagaacgag acgcactggt caggcgagag gtggaagatg
     5281 tcggtatcga ggaactcgag cacgatgacg cgcatctctt cgtagctccg gccgccaagc
     5341 tcggccacca tccggagcca gtagtcgttc tccaactccc tagtggccac ttctttgacg
     5401 acgtcgagta gcgtctgggt gatcagccgt tcgagctcgc cgaaggggtg ctgctcaaag
     5461 attgcacgga cgtggggttc tcgttcgggc ttgcacttgc ggaggaccaa cgcgcgtact
     5521 tcctggaaca agggggccgg ctggtccccc gattcacgct tcagctccgc ctttcgctgg
     5581 cagtccgcca accgcgccag ctctccctga ggcagcttca ggaacttatc catcttcgcg
     5641 tagatgtcgg tgcggttggg ggcgggcggc gccttcttgc gggtcagcaa ctgggagatg
     5701 taggactccg tcacatgggc ggctcgggcg agctctcgct ggtccaaccc aaactgctcc
     5761 agccgctgct tgatcaccag cgatacgtcc atgggaaacc gcctccgccg aaccggcgaa
     5821 ttaacagaaa taagttaagt aatcggcaat ctggtggcaa gtgtggcatt acacttgaca
     5881 agtacagtac ataaccataa attgggcatc ggtaaaccac gccagtaaag cccctcccaa
     5941 gctgaagtac cctaaggagt tcgatatgta cgcccggaaa gtctcgatcc gtctcaagcc
     6001 caatacatcc ggtgagttta cccggaccat cgacaacgac atcctccccc tgctgcgtaa
     6061 gcaggacggc ttcaaggatg agatcacgct cgtcgccccg gacggcgagg acgcagtcgc
     6121 catcagcctg tgggatcgca aggagagcgc ggatgcttac aaccgtgaga cctatcccca
     6181 ggtgctcaag ggtctagcga gggtgattga cggcacgccg gaagtgcagg tctacgaggt
     6241 tgccagctcg accttccacg ataccgccgc tcgcacctaa cccgacggtg gctttcggat
     6301 gagggggacg gcgctcgtcc gccctcttca tccccctccg tctccatgac gccgaaaatg
     6361 cctcagaagc tgaggtccgg cggttcatct cgaggaaagg tcggaatatg tacatgtcga
     6421 ttcttacttg ggtcctgctc ggtctgatcg tcggcttcgt ggcgagcaag atcgtgagca
     6481 aagctggcga gagcgtggcg ataaacgtcg cgctcggagt ggtgggcgcc ctcctcggcg
     6541 ggtggctgtt cgaccagttc ggcccgccgc cggtaacggg actgagctgg tggagcctgg
     6601 tgatggccgc aacctttgcg gtattcatcc tgatgggcta ccacacgatc gccgaaccac
     6661 gcgggcgacg gacgtgaaat accaccacgc ggacgtgaat taccgccacg tctgacccga
     6721 ggggaggcgt caatgtccgc cgctgccagg gccgaaccca aggtcaagat ccaaccgctt
     6781 cgagaccgcg tgctgatcct gccggctgag gaggcggaga cgatgcgggg gccgctgtac
     6841 atcccggata ccgccaagga aaaaccgaca cagggaaggg ttctcgccgt cgggcccggc
     6901 cggatctgga ggggcgaacg gatcaagctc gatgtggagg tgggggacac ggtgatctac
     6961 ggtcaataca gcggcatacc ctatcgggtt ggcgatgaca cgctgatcat catcaaggca
     7021 tccgacattc tggccaagat tggttgatat atggcagcga aagaactgct cttcagcatc
     7081 gaggcccgcg cgaagctcaa gcggggcgtg gaccgcctgg ctgaagcggt cagggtgacg
     7141 ctcggcccca agggccggaa tgtcgtcatc gacaagaagt tcggatcgcc caccgttacc
     7201 aaggacgggg tcacggtggc caaggagatc gagttggcgg acccggtcga aaacttgggc
     7261 gcccagatgg tgaaggaggt cgccaccaag accagcgatc tcgcgggcga cggcaccacc
     7321 accgccaccg tgcttgccca ggccattttc cgcgagggcg tcaagaacgt caccgccggc
     7381 gccaatccga tggagctcaa gcggggcatc gagaaggcgg tcgaggtggt ggtccaggag
     7441 ctcgagaagc tcagcgtccc ctcggccggc aagaaggaaa tcgcccaagt gggcgccatc
     7501 tccgcgaaca acgacaagga gatcggtgat ctcattgccg aggcaatgga caaggtcggc
     7561 aaagacggcg tcatcacggt cgaggaggca aagggcatcg agaccacgct cgagacggtg
     7621 gacggtaccc agttcgaccg gggctacctc tcgccctact tcgtcaccga tccggagaag
     7681 atggagacgg tgctggagga cggctatgtc ctcatccacg acaagaagat cagctcgatg
     7741 aaggagcttc tgcctatcct cgagaagtcg gcggaaagcg ggaagccgct cctcatcatt
     7801 gccgaggacg tcgagggcga ggcgctcgct acgctggtgg tcaacaagct ccgcggcacg
     7861 ctcaaggtcg tggccgtcaa ggctccgggc ttcggcgacc gccgcaagga gatgctccgc
     7921 gacatcgccg tgctcaccgg cggcgaggtg atctcggagg aagttggctt caagctcgag
     7981 aacacgacgc tcaaccagct cggccgtgcc aagcggatcg tggtcgataa ggacaacagc
     8041 acgatcgtgg acgggaaggg caaggccgcc gccatcacgg gccggatcaa cgagatcaaa
     8101 gtcgcgatcg agaagaccac cagcgactac gacaaagaga agctgcagga gcggctggcc
     8161 aagttggcgg gcggcgtggc gatcatcaat gtgggtgcgg cgaccgagac cgagatgaag
     8221 gaaaagaagg ctcgggtgga agacgccttg cacgctacgc gcgcagcggt ggaagaaggc
     8281 atcatcccgg gcggcggcgt tgccttcctg cgggcccagc ccgcgctgga ccggatcaag
     8341 ggcacggacg acgagaaggt aggcatcgcc atcgtgcgtc gcgcgctgga agagccgatc
     8401 cggatgatcg ctcagaacgc gggtgccgaa ggctcgatcg tcgtggccaa ggtcaaggca
     8461 tcgaaggaaa ggaacttcgg ctacaacgcc cagaccgacg cctacgagga cttagtgaag
     8521 gcgggcgtga tcgacccgac caaggtcgcc cgcaccgcgc tccagaacgc agcgtccata
     8581 gcggggttgc tgctcaccac cgagtgcgtg ctcaccgagc ggagggaaga gaaggcggca
     8641 ccggccatgc caggcggcgg catgggcggc atgtactgat cggccgctcg aactcgatgt
     8701 gaaccagcgc cccgtaaact agtttccggg gcgtttggct ttttgggacg ccgttcttca
     8761 tgaaggagtc atgcccaacc cccacgtgat aggccctctc ccgcccagca cgccgccgga
     8821 actcaggatc tcgcgcgacc agccgcgcgc ccttcccgcg gatctgctca gggacgcatc
     8881 tcgccggctg ggggtcatgt cgctcctcgc ggccacgctc tggttggtcg gctcggtggc
     8941 ggatcatctc gcgcttcggg cgataaccca cggggacccg cgctggctcc gcctggacgc
     9001 ggacgacgcg atcgcggcgg tgagcgtggt tctgtcgctc gggctgttct cctacactcg
     9061 cagggccgag cgtgaccccc ggttcgttct ggatctgggg ctcgtctaca tggtgcttac
     9121 cgcagcggcg atcggcctga tgatccactg ggaccccgtg ccgaaagatt ggcacgtgtc
     9181 gcccatgatc acctggatcg gagcggtcgt gctgatgttt gccgcgatcg tcccgagcac
     9241 ccccaccaag accttggtag cggcgctcat cgccgtctcc atgaaccctc tgggcatgct
     9301 gatagcgaga tcccggggaa cctgggagtt cggctcggcg agcaacgtgc tgctgatgca
     9361 ctatcccgac tacctgctcg ccggggtcgc ggcggtgatt tcctacgtcg tgaccggcct
     9421 gggccagcag atcgccaggg cgcgggagat ggggagctac cagctgggcg agttgctggg
     9481 acgcggcggc atgggagaag tctaccaggc tacgcaccgc atgttcgccc ggccggcggc
     9541 gatcaagctg atcc
//
LOCUS       DASRJQ010000184         3834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_536046, whole genome shotgun sequence.
ACCESSION   DASRJQ010000184 DASRJQ010000000
VERSION     DASRJQ010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536046"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /locus_tag="VFB89_04195"
     CDS             <1..1250
                     /locus_tag="VFB89_04195"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZM26530.1"
                     /translation="LQGAGRGLGAVLRETAGRGGGGRRWAWVRESLVVGQALISALVL
                     VAGGLLVRSLQRARALDTGFRLDSAVTFTVDPELSPLYDPARTQQFYRRLEADLAALP
                     GVRASARAATIPLDGSGSVRRVLPEGDATDFATAPVAEYNLITPGFLAALGTPLLEGR
                     EFTRADTAAPEEPAIVNAVLAQRLWPHESALGKRFRLQSPTGPLLVVVGVARASLYRT
                     LGERPRSALWRSLDRNPRSRTVVLVRAGGSTVGLAAAVHSTVRAIDPSLPVGGLGTLR
                     QHVSVAYASVESAAAGALAFGGLGLLLAASGIYGIITYAVSQRRREIGIRMALGAPGP
                     SVLRLVAGRALVLTLAGALIGGLFVALVPMGLDSLLHGVGRDDLAALLSATALFSAVA
                     VLAALSAALRAVRLNPMRVLRLD"
     gene            1372..1695
                     /locus_tag="VFB89_04200"
     CDS             1372..1695
                     /locus_tag="VFB89_04200"
                     /inference="COORDINATES: protein motif:HMM:NF022643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2834 domain-containing protein"
                     /protein_id="HZM26531.1"
                     /translation="MKPRHLYALLAVVGTVFPLWRFLPFLRDHGLNPSLFVQQLFSTP
                     VSGFFAMDVLISTLVLWLFVYFEGRRISIPHRWAPVLATIVVGVSLGLPLFLYLRELQ
                     LERRA"
     gene            1679..2488
                     /locus_tag="VFB89_04205"
     CDS             1679..2488
                     /locus_tag="VFB89_04205"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZM26532.1"
                     /translation="MNAGREPFVREAGAGPGVVCLHANASTSSQWRALMDLLAPTFRV
                     LAPDLYGAGKSPEWPSDRSISLHDEAAFIEPVLARAQPPVALVGHSYGAAVALVTALI
                     APIRIRALVLYEPTLFSVLDQVTPPPNEADGIRTVVREASRAVEAGDHDAAARRFIDY
                     WMGAGSWEQMPEQRKVPIAASIANIGRWGHALLTAPTPVEAIRSLQVPVLYLVGKSTP
                     SAAGGVARILTSTLPNVEVVEFEGLGHMGPVTHPEVVNPVIAGFLWRAADG"
     gene            3097..3435
                     /locus_tag="VFB89_04210"
     CDS             3097..3435
                     /locus_tag="VFB89_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26533.1"
                     /translation="MRTEGTDVEAVSGAVDGCGEDGLLRINDRRLTLQVTSVPSCSTF
                     WRDAAQGSTSTEVANAKAVEWLREAVLNKSSVDDRQGTILAIDARHAGVLADAAVADA
                     YSASVPEPDD"
BASE COUNT          555 a         1247 c         1305 g          727 t
ORIGIN      
        1 cactgcaagg ggcgggacgc gggctcggcg cggtgctgcg cgaaaccgcc ggccgcggag
       61 gcggcggacg ccgctgggca tgggttcgcg agtcgctcgt cgtgggacag gcgctgatct
      121 cggcgctggt gctggtggcc ggcggactcc tggtgcgcag tctgcagcgg gcgcgggccc
      181 tggacaccgg tttcagactc gacagcgcgg tcaccttcac ggtcgatccc gagctgtcgc
      241 cgctgtacga tccggcgcgg acgcagcagt tctaccgccg gctggaggcg gacctcgctg
      301 cgttgccggg ggttcgcgcc agcgcccggg ccgcgacgat tccgctcgac ggctccggca
      361 gcgttcgacg ggtactgccc gagggcgacg cgacggactt cgccacggcg ccggtcgcgg
      421 agtacaacct catcaccccc gggttcctgg ccgcgttagg cactccgcta ctcgagggtc
      481 gcgagtttac tcgggccgac accgccgctc cggaagagcc cgccattgtc aacgcggtgc
      541 tcgcgcagcg actctggccc cacgaatccg cgctcggcaa acggttccgc ctgcagtccc
      601 ccactggtcc gctgctcgtg gtggtcggtg tcgcgcgcgc gtcgttgtac cggaccctgg
      661 gggagcggcc ccgctccgcc ctctggcgca gcctcgatcg caatccccgc tcccggacgg
      721 tggtgctggt gcgggccggc ggctcgaccg ttggactcgc cgcggcggtc cacagcaccg
      781 tgcgcgcgat cgatcccagc ctgccagtcg gcggactcgg caccctccgc cagcacgtct
      841 cggtcgccta tgcgtcggtc gagagcgccg ccgccggcgc cctggcgttt ggcgggctcg
      901 gtttgttgct cgccgcgtcc gggatctacg gcatcatcac gtacgcggtc tcgcagcgcc
      961 ggcgggaaat cggcatccgc atggcgctcg gcgcgcccgg tccgtcggtg ctgcggctgg
     1021 tggccggccg cgcgttggtc ttgacgctcg ccggtgcgct gatcggtggg ctgttcgtcg
     1081 ccctcgtccc gatgggactg gattcgctgt tacacggcgt cggccgtgac gatctggctg
     1141 ccctgctgtc ggccaccgcg ctgttcagcg ccgtcgctgt ccttgccgcg ctgtcggcgg
     1201 cgctgcgggc cgtccgcctc aacccgatgc gcgtgttgcg gctggactga ccgcagggag
     1261 cttgcggcgg gtgtgctggc cgagcctccc ggaaacgcta ccttgcaatg aagcgcctcg
     1321 cgttcaccgc ctttgtcctt gcctgaggcc cacccaagag gaataccccg catgaagccc
     1381 aggcacctgt atgccctgct ggcggtggtc ggcaccgtgt ttcccctctg gcgtttcctg
     1441 ccatttcttc gggaccacgg gctcaacccc tcgctcttcg tccagcagct gttctcgacc
     1501 ccggtgagcg gattctttgc catggacgtt ctaatctcga ccctcgttct ctggctcttc
     1561 gtgtatttcg aaggacgtcg catctcgatc ccgcaccggt gggctccggt gctggccaca
     1621 atcgtagtcg gagtgtcgct cggcttgccg ctctttctct atctgcggga gctgcagctt
     1681 gaacgccggg cgtgagccct tcgtgcgtga agccggcgcc ggtcccggag tcgtctgcct
     1741 tcacgcgaac gcgagcacct ccagccaatg gcgcgccctg atggacctgc tggcgccgac
     1801 gttccgggtc ctcgcgcccg atctctacgg cgccggcaag agcccggagt ggccttcgga
     1861 ccgaagcatc agcctccatg acgaggccgc gttcatcgag cccgtgctcg cgcgggctca
     1921 gccgccggtt gcactggtcg gacattccta tggcgccgcg gtcgccctgg tgacggcgtt
     1981 gatcgccccg attcggattc gagcgctggt tctctacgag ccgactctgt tctcggtgct
     2041 ggaccaggtg actccgccac ccaatgaagc cgacggcatc cggacggtgg tgagggaggc
     2101 cagccgagcc gtggaggcgg gcgaccatga tgccgccgcc cggcgtttca ttgattattg
     2161 gatgggtgcc ggcagctggg agcagatgcc ggagcaacgc aaggtgccga ttgcggcatc
     2221 gatcgcgaac atagggcgat gggggcatgc cctgctgacc gcaccgacac cggtcgaggc
     2281 gattcgctcg ctccaggtcc ccgttctcta cctggtcggc aaatccacac cgtcggcggc
     2341 aggtggagtc gctcggatcc ttaccagcac cctcccgaac gtggaagtgg tggagttcga
     2401 ggggctgggg catatgggcc cggtcaccca tcccgaggtg gtgaatccgg tgattgcggg
     2461 gttcttgtgg cgggcagcgg atggctgacg cattcgccta caccgctcga agcaaaccgg
     2521 cgttcagaag ggacctgatc ctgtgggcta cgtgcggtgt ggtcgccttt gcgttcgaga
     2581 gaaaggccca gaagccaccc cgccgcgtga ggggccctgg gccagcatct ccgaatcctg
     2641 ggggtaccgg gagacggcag ggggtgagcg agagcgagcc ccctgtcggc tttctggacg
     2701 gcgcggtgtt tcctaattag gctgcataga gtgcgggtcc aatactagtt aggtggcaac
     2761 cgccgtgaga gttccatgaa tcgccataca gtttgctcac attgcggctc agagcgcagc
     2821 gaggacgccc cagtgtgccc gcactgcggt tccgttcgta agctggtgat gcttgcgatg
     2881 ggtggggaac tgaaagagtc ggggggcatg gtgggcttcg tccatgattc gccagctgct
     2941 gcggataagg cgcactccat ttccgttcac actccacttg gagcacgatc agaggcgcac
     3001 ctccaacctg atggtacggt tcgggtggcc gcttcgggac tgcccgacgt cggaactcgt
     3061 ggcgaggctc acgtcaaaga aatactgacg cggcgcatga gaaccgaagg caccgatgtg
     3121 gaggccgtca gcggtgcggt tgacggttgc ggggaggatg gcctgttgag gattaacgac
     3181 aggaggctca cgctccaggt cacctcggtc ccatcctgca gtactttctg gcgggatgca
     3241 gctcaggggt ccacaagcac tgaagtggcg aatgcgaagg cagtagagtg gcttagggag
     3301 gctgtgctca acaagtcatc agtcgacgat cggcagggca caattttggc tattgatgcc
     3361 aggcacgctg gggtgctcgc ggatgccgcg gtggccgacg cgtattcggc gtcggtaccc
     3421 gagcccgacg actgagtacg ggttcgcaca agtttgggtc gtgggcccta ctccgagcca
     3481 ctgtactcga ctgtaagttg ccacctaaca agcgcttgct gctgtcgggg cgtgggcgcc
     3541 tcagccgccg caaggtatgt tgatggagct ggttgcggcg gcttcggcac gccccgcagc
     3601 agaagcgcga gtcgttagac agcaacatcg gcgcatgcag ttgggcctgc ctccctatac
     3661 tctggagccg cggtggccac caccaagatc cttcgcttct gtctcctgct aggcgtaccg
     3721 agcgcgacag gagtcaccgg atcactcttg gctcaaggcc aatcagcgca cccgtcttgg
     3781 aactccgtta tcgtgcatcg ctccgctctg tcgtcgcgta tcgctggctc gcag
//
LOCUS       DASRJQ010000185         2013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3539726, whole genome shotgun sequence.
ACCESSION   DASRJQ010000185 DASRJQ010000000
VERSION     DASRJQ010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3539726"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..417
                     /locus_tag="VFB89_04215"
     CDS             40..417
                     /locus_tag="VFB89_04215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZM26534.1"
                     /translation="MVRCLIVDDSLQFLTAARGWLEREGIAVVGVASTIAQALQLAQE
                     LHPDVVLVDIDLGGESGFELASQLQRKAGVNSSRVILISAYAEEDYAELIAASPAVGF
                     LPKTALSGQRIRDLVEDGAGQGV"
     gene            complement(428..1135)
                     /locus_tag="VFB89_04220"
     CDS             complement(428..1135)
                     /locus_tag="VFB89_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZM26535.1"
                     /translation="MTMVRGRIGPTGDHGKGNQPTMRIVLAEDELLLREGMASLLERS
                     GFEVVGQAGDAVQLLALVGDTSPDLVVVDIRMPPTRTREGLEAAREIRQQHPDIGILV
                     LSAHVEVEHAVELLASGRGIGYLLKSRVTDVAEFVDTLERIAKGGSVVDPALVQELVS
                     VRRRIDPLAALSERERDVLALMAEGRSNAGIARQLWVTEGTVEKHVRSILAKLNLPEA
                     EEDHRRVLAVVTFLQAQ"
     gene            1534..>2013
                     /locus_tag="VFB89_04225"
     CDS             1534..>2013
                     /locus_tag="VFB89_04225"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZM26536.1"
                     /translation="MAGRYRLRALLGRGGMGRVWLAEDELLHRPVALKQDIASDPASD
                     GWHRTAPGRLLIEACSAATVNHKGAVTIYDVVTDNSRTWIVMEPLSGRTLAESITAEG
                     SLSIARVTHIGLRLLDVLMAAHSVGILHCDVKPANVHLCDDGRVVLTDFGIACSMLDE
                     "
BASE COUNT          356 a          645 c          641 g          371 t
ORIGIN      
        1 cggaatgtgc attctgaacc gatacggcag tcttgatcca tggtgcggtg cctgatcgtt
       61 gatgacagct tgcagttcct cacagctgca cgtggctggc tcgaacgcga gggaatcgcc
      121 gtggtcggcg tggcttcgac catcgcacag gcgctgcaac tggcgcagga gctgcatccg
      181 gacgtagtgc tggtcgacat cgatctgggc ggtgaaagtg gcttcgagct ggccagccag
      241 ctccagcgaa aagcaggtgt gaattcgtca cgggtcatat tgatctccgc atacgccgaa
      301 gaggactacg ccgagctgat cgcggccagc ccggctgtcg ggttccttcc caagacggcc
      361 ctatcggggc agaggatccg cgacttggtt gaggatgggg ctggtcaggg ggtataggtg
      421 gccatagcta ctgggcctgg aggaaggtga caaccgccag gacacggcga tgatcttcct
      481 cggcttcggg caggttcaac ttcgcaagga tgcttcggac gtgcttctcg accgttccct
      541 cggtgaccca caactgcctg gcgattcccg cattggagcg cccctcggcc atcagcgcaa
      601 gcacgtcacg ctcgcgctca ctgagcgcgg ccaatgggtc aatgcgccgg cggaccgaga
      661 cgagctcttg caccagcgcg gggtcgacga ctgagccccc cttcgcgatg cgttcgagcg
      721 tgtcgacgaa ttccgctacg tcggtgacgc ggctcttgag cagatagccg atcccccggc
      781 cgctcgccaa cagctccacg gcgtgctcca cttcgacatg agccgacaag accaaaatgc
      841 cgatgtctgg gtgctgctgg cggatttccc gtgccgcctc cagcccttcc cgagtccgcg
      901 tgggaggcat acggatgtca accaccacaa gatccggcga ggtgtcaccg accagcgcaa
      961 gcagttggac tgcgtcgcca gcctgaccca ccacctcgaa gccggagcgt tcgagcaggc
     1021 tcgccattcc ctcacggagc agaagctcat cttctgccag cactatgcgc atcgtcggtt
     1081 ggtttccctt gccatggtct cccgtggggc caatgcgccc acggaccatc gtcatggtcg
     1141 catgattggc tagcctcact gaagtgagcc cgtcgcggag ttggcgagag gcggcctgat
     1201 gcgcggacgc tcataactgg cggtgatttg gcgccacggc gttctgtcgt gctgttcttg
     1261 gtagccgacc caccaaatcg caacgtcgcg cttagcggtc atacgtctgg gatctggcag
     1321 cagcccgtcg agtgaaaacc cctgccagcc ggtatcaagg atgggcgcct gctgcgacga
     1381 catgcgctgc aagttccacc atcgtggagc ggtatgaagc cgctcttgga ctcccccacg
     1441 agcaaacgac atctccggcc agtagagcct gcggaccgga gagttgggtt accgattgcc
     1501 cgacgcgtgc cggcgtcgcc tggagctcgc ctcgtggccg gccggtaccg gctccgggcg
     1561 ctgctgggcc gagggggtat gggcagggtg tggctggcgg aggacgagct attgcaccga
     1621 cccgtcgccc ttaaacagga catcgcgagc gacccggcgt ccgacggctg gcatcggact
     1681 gcgcccggcc gtctcttgat cgaggcgtgc tccgccgcca ccgtgaacca caagggagcc
     1741 gtcacgatct atgacgtcgt gaccgacaac agccgaacct ggattgtcat ggagccgctg
     1801 tccgggcgaa cgctcgccga gtcaatcacc gcggaggggt ccctttccat cgctcgggtg
     1861 acgcacatcg gccttcgcct gctcgatgtg ctcatggcag cccacagtgt cggcatactg
     1921 cactgcgacg tcaaacccgc caatgtgcac ctctgcgacg acggccgggt agtgctcacc
     1981 gacttcggca ttgcctgcag catgctggac gag
//
LOCUS       DASRJQ010000186         4251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_439345, whole genome shotgun sequence.
ACCESSION   DASRJQ010000186 DASRJQ010000000
VERSION     DASRJQ010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439345"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VFB89_04230"
     CDS             <1..285
                     /locus_tag="VFB89_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26537.1"
                     /translation="RIMGPSGLGATPKIMGLNEGDPYQISGAAGFFEGTVLVNGPPFE
                     FSGTVSNLNDALLRYELYGGRAKLWVATWGVEESRVAQVEAGLRKCLEAL"
     gene            complement(315..1553)
                     /locus_tag="VFB89_04235"
     CDS             complement(315..1553)
                     /locus_tag="VFB89_04235"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM26538.1"
                     /translation="MVLPNVREGVVIALDAMRANKLRSLLTILGVVIGVTTVMAIASM
                     VQGIRTQIFNAIEVAGPTAFYVVRFFSQTPLNPDRLPYEVRIRPVLETTDADAMQRIP
                     QIGYAGMWVQLFERVEYGGERTQTVTVFGADDGFMNIQGGALLRGRFFSRAELVGEPV
                     VVLDNEIAERLFGQIDAVGRFVRIGGKSLRVIGIWERPGNIFEPPGQQTAGVMPFEYA
                     RQNYRYDNTNALFIAAKPQLGVSVDDAKDAVISALRRVRNLRPGMPDTFDLITQDQIL
                     AVVSNFTSYFFIAMVALSSVALLVGGIGVMAIMMVSVTDRTREIGLRKALGATRREIL
                     WQFLVEAATLTSIGGIIGIAMGLGTGELLKLALRIESQAPLWSAVLAAGVSIGIGLTF
                     GLYPANRAARMDPVEALRHE"
     gene            complement(1556..2800)
                     /locus_tag="VFB89_04240"
     CDS             complement(1556..2800)
                     /locus_tag="VFB89_04240"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM26539.1"
                     /translation="MPLSEALRLAIGSILGSKLRSFFTLLGIIVSVGFLVVVVAIIQG
                     MNAYVKENLTGAMIGTNAFQVRRNPISVGLMDDEQLKAIAKRPLVTREDAEVVQRAVP
                     DAQAVALQSGWPTPASDVEYGNRTVTNVLTFGVTPPYQLVQDYRFAYGSPLTDPDVHE
                     RRYAVVLGWDIAHELFDDPAIAVGKKVRVAGYELEVKGVIAKKGRVLGQSFDGFVLIP
                     FSTFESMFGRRKTTVVSVKMANPDAIEDAMARTEEAMRVAHRLRPGEANDFTVDKADA
                     LVAFWKGLTRVLFTAIPAVVGIGIVVGGIVIMNIMLMSVNERTREIGLRKSLGARRRD
                     IRRQFLVESVILSALGGLFGILAGWALAVAVAQFTPLPARITAWSVGVALALGAGTGI
                     IFGVYPAGRAARLDPISALRVE"
     gene            complement(2890..4113)
                     /locus_tag="VFB89_04245"
     CDS             complement(2890..4113)
                     /locus_tag="VFB89_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM26540.1"
                     /translation="MEGVGIALDSIRANKVRAALTILGVAIGVTVVIAMGAAITGINR
                     SISQILEAAGPKTFFVFRFFQGGITVSDGSEETQPWRRNPWLTVEEADMLRQLPAIRD
                     VNVDEHAQGPVSWGDANLKGVSMAGMGPHWIQVNGGDILEGRNFTEAEYAANARVVVI
                     NDHLAKALMPGVDPIGKEVKIFGEPFTVVGMHAEAASLFSNADKPRLAIPHTTFVRVA
                     EYEKGFMDFAVVPTDSATLQEAQDEVVAALRSVRGLRPGEDNNFAVVTQNKLLDTFNS
                     ITAGFFIAMIALSSVGLMVGGVGVVAIMMISVTERTREIGVRKALGATRGEIMFQFLV
                     EAATLTLVGCLIGMALGSLAAWAVRSFSPVPATVPVASVVAALVASILTGVLFGIYPA
                     SKASRLDPVEALRYE"
BASE COUNT          841 a         1333 c         1320 g          757 t
ORIGIN      
        1 cgcatcatgg gcccgagtgg tctgggtgca actccgaaga tcatggggct caacgaaggc
       61 gatccctacc aaatctcggg tgccgccgga ttcttcgagg gaaccgtact ggtgaatgga
      121 ccgccgttcg agttctccgg cactgtcagc aacctgaacg atgcgctgct gcgttatgag
      181 ttgtacggcg gacgagccaa gctttgggtg gccacctggg gggttgagga gtcgcgcgtc
      241 gcacaggtgg aggccggatt gaggaagtgt ttggaggcgc tgtgaaaatg ctgcagcgtt
      301 gccggcggcg ctgctcattc gtgtcgcagc gcctccaccg gatccattcg ggcggctcga
      361 ttcgctgggt acaaaccgaa cgtgagtccg attccgatac tgacgccggc ggcgaggacg
      421 gcgctccaga gcggcgcttg cgattcgatt cgaagggcca acttgagcag ctcgccggtg
      481 ccaagcccca tagcgatccc gatgattccg ccgatggagg tgagtgtggc cgcttcgacc
      541 aggaactgcc acagaatttc tcgacgggtg gcgcccagcg ccttgcggag accgatctcg
      601 cgggttcgat cggtcaccga taccatcatg atggccatga caccgatgcc accaaccaga
      661 agcgccacac tggagagtgc caccatcgca atgaagaagt acgaagtgaa gttgctcacc
      721 acggcgagga tctgatcctg ggtgatcagg tcaaaggtgt cgggcatacc cgggcgcagg
      781 ttgcgcaccc gccgcagtgc cgaaatgact gcgtctttgg cgtcgtcgac tgacacccca
      841 agctgtggct tggccgcgat gaaaagggcg ttggtattgt cgtatcggta attctggcgc
      901 gcatattcga acggcatcac gcccgcagtc tgctggcctg gcggctcaaa gatgttgcct
      961 ggccgttccc agatgccgat caccctgagc gacttgccgc cgattctcac gaaccggcct
     1021 acggcatcga tctggccgaa tagccgttcc gcaatctcgt tgtccagaac caccaccggc
     1081 tcacccacca attcggcgcg actgaaaaag cgtccgcgga gcaacgctcc accctggata
     1141 ttcatgaagc catcgtctgc gccgaacacg gttaccgtct gggttcgctc tcccccgtat
     1201 tccacccgct caaacagctg aacccacatc ccggcgtacc cgatctgcgg gattcgctgc
     1261 atggcatcgg cgtcggtcgt ttcgagtacc ggcctgatgc ggacctcgta cggcaggcga
     1321 tcgggattga gcggggtctg ggaaaagaac cggacgacat agaacgccgt tggcccggcg
     1381 acttctatgg cgttgaatat ctgagtgcgg atgccctgga ccatcgaggc gatggccatg
     1441 accgtcgtca cgccgatgac gacgccgaga atcgtgagca gcgaccggag cttattggcg
     1501 cgcatcgcgt cgagcgcaat gaccacgccc tctcgaacgt tgggcagaac catgactatt
     1561 cgacccggag cgcgctgatc gggtcgagcc gggcggcgcg tccggcggga tagaccccga
     1621 agatgatccc ggtgccggcg ccaagggcca gggccacgcc cacggaccag gccgtgattc
     1681 tcgcggggag gggtgtgaac tgcgcgacgg ccacggccag cgcccacccc gccagaatgc
     1741 cgaacaggcc tcccagcgcg gagagtatta ccgactccac cagaaactga cgccgaatat
     1801 cccgccgccg cgcgcctagg gacttgcgca ggcctatctc gcgagtgcgc tcgttcacgc
     1861 tcatcagcat gatgttcatg atgacgatgc cgcccaccac gattccgatt ccaacgacgg
     1921 ccggtattgc agtgaacaac acgcgagtga gccctttcca gaacgccacc agggcatcgg
     1981 ctttatcgac ggtaaaatca ttggcctcgc cgggacgaag acggtgcgcg acccgcatgg
     2041 cttcctcggt acgggccatg gcgtcttcga ttgcgtcggg attggccatc ttgactgaga
     2101 cgactgtcgt ctttcgccgc ccgaacatcg attcgaatgt gctgaaggga atcagcacaa
     2161 agccgtcgaa cgactgcccg agcacccgac ccttcttcgc gatgacaccc tttacctcga
     2221 gctcgtagcc ggccacccgg accttctttc ccaccgcgat ggcgggatcg tcgaacagct
     2281 cgtgggcaat gtcccaccca agcacgacgg cgtaccggcg ctcgtgaacg tcgggatccg
     2341 tgagcggaga cccataggca aagcggtaat cctggaccag ctgatagggc ggggtgacgc
     2401 cgaaggtgag cacgttagtg accgttcgat tgccatactc cacgtcgctg gccggcgtgg
     2461 gccagccgga ctgcagtgcc acggcctggg catccggcac cgcccgctgc acgacttccg
     2521 cgtcctcccg cgtgaccagc ggacgtttgg cgatggcttt gagctgctcg tcatccatca
     2581 ggccgacgga aatcggatta cggcgcacct ggaaggcatt ggttccgatc attgccccgg
     2641 tgaggttctc cttcacatag gcgttcatcc cctggatgat cgcgacaacc acgaccagga
     2701 accctaccga gacgatgatg ccaagcagag tgaagaagga acggagcttg gagccgagaa
     2761 tagacccgat tgcgaggcgc agggcttcgg agagaggcat gaatagaagc gtaacggtaa
     2821 acggtaaacg gtgaacaggg gctcgcgtgc gtcggagccc cgttcaccgt tcactgttca
     2881 cgctttttct tactcgtatc tcagcgcttc gaccggatcc agtctggacg ccttgctggc
     2941 aggatagatc ccgaacaaca caccggtgag aatggaggcc acaagcgcgg ccaccaccga
     3001 ggctactggc accgtcgccg gcacggggct gaaggagcgt accgcccacg cagccaacga
     3061 cccgagcgcc attccaatga ggcacccgac cagggtcagg gtcgctgctt caaccagaaa
     3121 ttgaaacatg atctcgcccc gggtggcgcc cagagccttc cggacgccga tctcgcgggt
     3181 ccgttcggta acggagatca tcatgatcgc gacgacgcca acgccgccga ccatgaggcc
     3241 cacgctcgaa agggcgatca tggcaatgaa gaacccggcg gtgatactgt tgaacgtgtc
     3301 cagcagttta ttctgggtca cgaccgcgaa gttgttgtcc tcgccgggtc gcaggccgcg
     3361 cacgcttcgc agggccgcca cgacttcgtc ctgagcctcc tgcaacgtgg ccgagtcggt
     3421 gggaacgacc gcaaagtcca taaaaccctt ttcgtactcg gcgactctca cgaacgtggt
     3481 atgaggaatc gcgagtctcg gcttgtcggc attggagaag agagacgcgg cctctgcgtg
     3541 catgccgacg accgtgaagg gctcgccgaa gatcttgacc tctttcccga tgggatcgac
     3601 gccgggcatg agagcctttg ccagatggtc gttgatgacc acgacccgcg cgttcgcggc
     3661 atactccgct tcggtgaagt tgcggccctc gaggatgtcg cccccattca cctggatcca
     3721 gtgcggtccc atgccggcca tggagacacc cttcaggttt gcgtcccccc agctgaccgg
     3781 tccctgggcg tgctcgtcga cgttcacatc gcggatggcc ggcagctgcc gcagcatgtc
     3841 cgcctcctcc acggtgagcc acggattgcg gcgccagggc tgagtttcct ccgagccatc
     3901 ggaaacggtt atgccgccct ggaaaaaccg gaacacgaaa aaggtcttcg ggccggcggc
     3961 ctcgagaatc tggctgatgc tgcgattgat cccggtgatg gccgctccca tggcgatgac
     4021 cacggtgaca ccgatcgcca cgccgagaat ggtgagggcg gcccgcactt tgttggcgcg
     4081 gatcgagtcc agggcgatgc ccacgccttc cattagtcga gaaagggtgg aagcggagcg
     4141 ttgcggtgtc attcggccct caaggcaacg ataggatcga gccgggaggc tcggctcgcc
     4201 ggatagacgc ccgccgccac tccaacggcg acgcccagga acacggcgag t
//
LOCUS       DASRJQ010000187        10900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3537104, whole genome shotgun sequence.
ACCESSION   DASRJQ010000187 DASRJQ010000000
VERSION     DASRJQ010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 10900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10900
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3537104"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VFB89_04250"
     CDS             complement(<1..466)
                     /locus_tag="VFB89_04250"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZM26541.1"
                     /translation="MSTRPANQAFPSRPSRLVPTARELKDLLRLAVPVVVVQVGIMLM
                     GVVDSIMVGHLSANALASVALGNMYFMGLAIFGMGVLLALDPLVAQAVGAGDHTAIAR
                     ALQRGLVLALVLSVPCGALLLTVDPVLSAIGEPPELIPYASLFTSIMIPSVVP"
     gene            695..982
                     /locus_tag="VFB89_04255"
     CDS             695..982
                     /locus_tag="VFB89_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26542.1"
                     /translation="MSSVTAPQVTQPLSYGPGASGPTLAAERLGYSNDFEQPTTVNAA
                     AKRMAKREGRALALVGGAAVIAGLLIGDDAGTVIAIGGAGLGLYGLYVWQR"
     gene            complement(979..1482)
                     /locus_tag="VFB89_04260"
     CDS             complement(979..1482)
                     /locus_tag="VFB89_04260"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26543.1"
                     /translation="MTGRRIIIGVIGPGEQATSRELEWARQIGVLVARAGWVTLTGGW
                     SLGVMDAACRGARDAGGLTVGLLPSTDGGGASSAIDLVLPTGMGEARNNLIVLASDAL
                     VCCGMSAGTASETALALRAGKPLVLLSPAPETLAFLRGLGRVAPIEAPDPEAAIAALR
                     RLVSSRS"
     gene            complement(1479..3089)
                     /locus_tag="VFB89_04265"
     CDS             complement(1479..3089)
                     /locus_tag="VFB89_04265"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZM26544.1"
                     /translation="MSTGPSSSASVPAANVLGRIGLPEPVSALAARTWDVIVVGGGHN
                     GLTCAAYLARAGKRVLVLEARQQIGGACTMEEPWPGVRMSPCAYVVGLLHPLVMKELD
                     LKGHGFEWHPAAGGLFVPFEDDSSIQLWNDDAKCEAEVRDFAPGDVDGWHAMLDVKRR
                     LRDALRPEGDGDLWIGPAPNRETIEQRLRGDPEARAMLFDWSMVELVERYLSDERLQM
                     AYLGQGVIGTDASPHDKGTASVYYHHASGRMDGLPGTWGYVIGGMGRVSFILCDVARE
                     AGAVVAAGVPVARIVAGEGVELDDGTRIRAGRVVSNADPRVTLRLLGNHADSGWKRQV
                     ESVPIEGVTVKVNLTLRELPNFTSRPGTFEPHHTGQVNTPLTKAEWLSQYRTAKAGDL
                     PPRLWCELYLQTAYDRSVAPSGVHTLSVFSQYVPYRFRDGTWDTRRDEVGRAVVASIA
                     RFCSNLPQAIIDLQVLGPPDIEQRVGLTGGHIFQGEILPQYMWDRRLAARTPMPGVYL
                     CGACTHPGGSVISINGRNAAMEVLRDLA"
     gene            complement(3317..4144)
                     /locus_tag="VFB89_04270"
     CDS             complement(3317..4144)
                     /locus_tag="VFB89_04270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZM26545.1"
                     /translation="MRTPSGGARPLALVTGATAGIGRVFADRLARRGYDLLLVSRDGQ
                     RLAAVARELSGLGISVEVLEADLSRDDDVARVAERIKTGNPLSLLVNNAGFGTKGLLI
                     ETPVEPQVAMLHLHVLAPMRLIQAALPGMLARRSGGVINVSSVAGFLFSPGSVNYCAS
                     KAYLTVFSEGLATELAGTGIRVQALCPGFTRSEFHQRMAMDSAALKRRRLWLTAEYVV
                     DTSLAALDRGGPVVFIPGFRYKLIAALLRLMPRRLLRYLMQKRQHPRSGRPVENAKF"
     gene            4444..6276
                     /locus_tag="VFB89_04275"
     CDS             4444..6276
                     /locus_tag="VFB89_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26546.1"
                     /translation="MYRIEFEPGDVGVFRSVEEVATAIKSGVITSRARIYHQATDKWL
                     PIEFHPHYKKALEMVARGNVPAPSSSTRFNGSPFTPPGARAAIQVAPPAPAVVSPPAP
                     IVVEAANETPPTINHVELEPPAATHFEPEPAAEVPLPPVEREPAPEPAVEMSNPAIVE
                     PEPAPPPPNFVFIPRSREIRFIPLEEPKPLGRSRQRHLEYPIPPAALAPEMPPFFVGA
                     PSDELRYQEVAPEVPQSEHPGNESLAIDTAGMDSLALAADASPRPIFRPRFRVGSLGK
                     LRRPMLIGVGALALIVSTRFGISAAASTLNAGVMTSGWGALPFLNRESGADRSGESLN
                     QAQAGGRLPRNPEPQGSPSFGSSSAFPIGRSGSGDSRRQPGGVNSATSPGEKIEQVAT
                     PDSMVRKAPVAQIAIAAPKIAGLPGATNVKLTPAVVVTHYEAAYAAARSELETGFRTA
                     GFANVFAPEHLNSAQGVRAARLAAGTASAYVAKYRRREAEIEAAYADSAAELAKTPAD
                     RKVWDGRKVLQESPEVAKLAGFLLQEIDSVFGVLASQDGAYEIKDGAIVFQDAAAARA
                     YAELRPWLERPAHQWADSAEGTPNSAGRVLRAIGSTRLPEGGAF"
     gene            complement(6295..6825)
                     /locus_tag="VFB89_04280"
     CDS             complement(6295..6825)
                     /locus_tag="VFB89_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26547.1"
                     /translation="MTRTFLGSSAGLLLLSVAVSAGRAQSADSPSQPVPTTSVADTAA
                     FDAERDSTCLAAAETLSLTEPQRKLFYRNVQTVVGEALAANPGGGRTVIDSAVSVVIR
                     ETGRKMKFLRQARLLFSEMFARRSGDEDRAPGTEEELWRSSRNVAGLIDLYRKNPDAS
                     DAELERAMAPAFRVSH"
     gene            complement(6909..7469)
                     /locus_tag="VFB89_04285"
     CDS             complement(6909..7469)
                     /locus_tag="VFB89_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26548.1"
                     /translation="MHTRYTCLAIPLALVVAACSSERSDSTPRDLTLLPSAESATDAV
                     ASARELDLPRAEPEQKNAAGASALTPVPARVHARHAKQVDRAAKDAPVTAPDPEPAPQ
                     SPAPGPRDVANAGGAGMPLDPGQTVTVLPVSGGTGTQPLPASDVATEDQIRRPWIVIG
                     DDRCIPGRGEVMPRGHRGFRVSRLGH"
     gene            complement(7645..8202)
                     /gene="efp"
                     /locus_tag="VFB89_04290"
     CDS             complement(7645..8202)
                     /gene="efp"
                     /locus_tag="VFB89_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HZM26549.1"
                     /translation="MKASDIRRGHVLIIDGAPCRVMDFQHRTPGNLRAFVQVRLRNLN
                     SGNTFDTRLSATEFVDEARLDTKELQVLYRDANGVHVMDNGTYEQYSLDDEVVGDAGS
                     WLESGMTFVAEWLNGRPIAIELPSAVEMEIVETAPIMKTATKTASTKPAKLSNGVTIQ
                     VPEFIGEGERVRVNPREGVYLERAK"
     gene            8404..8646
                     /locus_tag="VFB89_04295"
     CDS             8404..8646
                     /locus_tag="VFB89_04295"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HZM26550.1"
                     /translation="MSARNESELGADERIWEDVHERLMGHPDFDATDVEIEVEGGLVT
                     LAGRVDSRETKWLAEEITRAVQGVQDVHNRLKVARW"
     gene            complement(8648..10111)
                     /locus_tag="VFB89_04300"
     CDS             complement(8648..10111)
                     /locus_tag="VFB89_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HZM26551.1"
                     /translation="MTTATSTVPRRRRRGERVLYQRLADELVGLIETRTLRSGDRLPS
                     VRTYSRQKRVSLATVLRAYQLLEDRGYLEVRPQSGHYVSAAPVSRAPSPSIPRPSFTK
                     EEVRIADLAWELLARVNDKGMAPFGIATAAPEHYPTHRLGRIMTRVIREAGAAGLVYG
                     SPVGHEALLHQLARRALAAGLTLRPDELIVTNGCTEAIQLALRALTRPGDVILVESPS
                     YYGFPQTAESLDLRVVEVPTDPETGASLEHLDSAIRRTKPRVFLTNPNFQNPLGFAVP
                     DAVKREQVALLARHDVAVVEDDLYGDLYFGETRPAPLKAFDTTGNVILCSSFSKTLAP
                     GYRIGWVAAARHAGRIRQLKLASSIHTNTLAQLTVAEYLSGGGAEHHLRRLRQAFRAQ
                     VSDYAHAIARLFPQGTRVSRPKGGHLLWIELAPRTDTLALARSALARRIGIVPGRVFS
                     TGDTFGHCFRLNCGFPLRGELEQALETLAGLARSAEG"
     gene            10192..10674
                     /locus_tag="VFB89_04305"
     CDS             10192..10674
                     /locus_tag="VFB89_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZM26552.1"
                     /translation="MLDPTVLLDTRIVPYGPEHRNAFRDLNLEWIATYFEVEAEDRKI
                     LGDPETHVLGKGGAILMALEGEEPVGTGALIPTGSHEFELAKMAVTPRAQGRGVGRRL
                     CAALIDLARRMGAHRIELVSHRSLVPAIALYRSLSFREVPLGDVAYRRANIRMELRLR
                     "
     gene            10671..>10900
                     /locus_tag="VFB89_04310"
     CDS             10671..>10900
                     /locus_tag="VFB89_04310"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZM26553.1"
                     /translation="MRARIIAAFAVVYLVWGSTYLGIRWAVESFPPFVMTASRFLVAG
                     ALLYLLARLRGAPAPPRRAWLAAIVIGTLLVTG"
BASE COUNT         1943 a         3621 c         3530 g         1806 t
ORIGIN      
        1 acggcaccac gctcgggatc atgatgctgg tgaacagcga ggcgtacggg atcagctcgg
       61 gaggctcacc gatcgcagaa agcacggggt cgactgtcag cagcagtgcg ccgcagggaa
      121 ccgagagcac cagcgccagc accagcccac gctgtagcgc tcgcgcgatc gccgtgtgat
      181 cgccggcccc gactgcctgc gcgaccagcg gatcgagtgc cagcaggact cccatcccga
      241 agatcgccag gcccatgaag tacatgttgc ccagggccac gctcgccagc gcgttggccg
      301 acaagtgtcc caccatgatg gagtccacca cacccatcag catgataccc acctgcacga
      361 ccacgaccgg cacggccagt cgcagtaggt ccttcagttc gcgggcagtc ggtacgagtc
      421 tgctcgggcg cgaggggaac gcctggtttg cggggcgagt ggacatcagt tcagcacgac
      481 gacgtcggaa ggcgggaaac ataacgtggc aggcggtcat gggcatggaa gatgcaaccc
      541 cgtcagcgct ggtctcatcc gtgtgccgcc aatccgagcg agcacgtgag acaagtcacg
      601 ttttccgggg tacgatatgc gcttttctaa gtgcgttccg attggagttg cgttgtttct
      661 tgcgataatc ctctcgcagc ccgcggcggc acaaatgtcg tccgttaccg ctccgcaggt
      721 gactcagccg ctgtcctatg ggcccggcgc atcggggccc actctcgccg ccgagcgtct
      781 gggctattca aacgatttcg agcagccgac cacggtgaat gccgcggcca agcggatggc
      841 aaagcgagag ggtcgcgcgc tcgcgctggt cggcggcgca gccgtcattg cagggctctt
      901 gatcggagat gacgccggga ccgtgatcgc aatcgggggc gcgggactcg gcttgtacgg
      961 cctctacgtg tggcagcgct aggaccggct ggacaccaga cgccgaagcg ccgcgatcgc
     1021 cgcctccgga tcaggcgctt cgatcggggc cactcgcccc aggccgcgta gaaacgcgag
     1081 cgtttcaggc gcaggactca atagcacgag cggctttccg gcgcggaggg cgagtgccgt
     1141 ctccgacgcc gtgccggcgc tcattccgca gcacaccagc gcatcactcg caaggacgat
     1201 gagattgttc cgtgcctcgc ccatcccggt cggcagcacc aggtcgatcg cgctcgacgc
     1261 tcctcccccg tcggtgctcg gcaagagacc cacggtaagc ccgccggcgt ctcgcgctcc
     1321 tcgacacgcg gcatccatca cgcccagact ccagccgccg gtcagcgtca cccaccccgc
     1381 cctggcgacg agcaccccaa tctgacgcgc ccattccagc tcgcgtgagg tcgcctgctc
     1441 acccggtccg atgacgccga tgatgatgcg acggccggtc atgccagatc gcgcaggact
     1501 tccatagcgg cgttgcgtcc gttgatcgag atgacgctgc cgcccggatg ggtgcaagcg
     1561 ccgcagagat aaacgccagg cattggcgta cgagccgcta gccgccgatc ccacatgtat
     1621 tgcggcagaa tctcgccctg gaagatgtgc ccgccggtga gtcccactcg ctgctctatg
     1681 tccggaggcc cgagcacctg cagatcgatg atcgcctgcg ggaggttgga gcagaaacga
     1741 gcgatagacg ccacaaccgc ccgaccgacc tcgtcgcggc gggtatccca ggttccgtca
     1801 cggaagcggt agggcacgta ctgggagaac acgctcaggg tgtgaacgcc cgagggcgcg
     1861 acgctgcgat cataagccgt ctgcagatag agctcgcacc agagcctcgg cggcaggtcg
     1921 cctgccttcg cggtgcgata ctgcgacagc cattccgcct tggtgagcgg ggtgttcacc
     1981 tggccggtgt ggtgcggctc gaaggtgccc ggccgcgagg tgaaattggg aagctcccgg
     2041 agcgtgagat ttaccttgac ggtgacgccc tcgatcggca ctgactccac ctgacgcttc
     2101 catccggaat ctgcgtggtt gcccaggaga cgcagggtga cccgcggatc cgcgttcgac
     2161 accactcggc ctgctcgaat cctggtgccg tcgtccagct cgacgccctc accagctacg
     2221 attcgagcaa ccggtactcc cgcagcgacc accgccccgg cctcccgggc aacgtcgcac
     2281 agaatgaacg acacccgacc catgcccccg atgacataac cccacgttcc cggcaagccg
     2341 tccatgcgac cggacgcgtg atggtagtac accgaggccg ttcccttgtc gtgcggactc
     2401 gcatcggttc cgatcacgcc ctgccccaag tacgccatct ggaggcgttc gtcggacagg
     2461 tatcgctcga ccagctcgac catcgaccag tcgaacagca tggcccgcgc ttcgggatca
     2521 ccccgcaggc gctgctcgat cgtctcccgg tttggcgccg ggccgatcca gagatcgcca
     2581 tcgccctcgg ggcgaagcgc gtcccgcagc cggcgtttga cgtcgagcat cgcgtgccac
     2641 ccatccacgt cgcccggggc aaagtcgcgc acctcagcct cgcacttcgc gtcgtcgttc
     2701 cacagctgaa tgctggagtc gtcctcgaag ggcacgaaaa gaccaccggc ggccggatgc
     2761 cattcgaatc cgtggccctt gaggtccagc tccttcatca ccagtgggtg caagagaccg
     2821 acgacgtagg cgcagggaga catgcgcacg cccggccagg gctcttccat cgtgcatgcg
     2881 cccccgattt gctgtcgtgc ctcgaggacg agtacgcgtt ttcccgcccg ggccaggtag
     2941 gccgcacaag tcaatccgtt atgccccccg ccgaccacga tgacgtccca ggtccgcgcg
     3001 gcgagcgcgc tgaccggctc gggaaggcca atgcgtccca gcacattggc agcgggtacc
     3061 gaggctgagg aagatgggcc agtcgacacg gcttatcccg ggtttggaga gcgaagcgaa
     3121 tcgggcagaa gtctaacagc aggcgcaagc caaccatccg acaaccgtta cgggcgactg
     3181 gccgtctaat gccgtgtctc ccaccgaggc tccctcgtgc tccctgggcg ttcgccgagc
     3241 acgaaggcca gacgattgaa gtacgactgc aatgcaaaga cggcgtggtg agtggacgct
     3301 tcctcggcgg atggggtcaa aacttcgcgt tctcgacggg tcggccgctg cgcgggtgct
     3361 ggcgtttctg catcaggtac cgcagtagcc gacgaggcat gagccggagt aaggccgcga
     3421 tcagcttata gcgaaagccg ggaatgaaga ccaccggtcc tccgcggtcc agcgccgcga
     3481 gcgacgtgtc cacgacatac tcggccgtga gccagaggcg ccgccgtttg agtgcggcag
     3541 agtccatagc catccgctga tggaattcgc ttcgagtgaa cccgggacaa agcgcctgaa
     3601 cccgtatgcc ggtccccgcc agctcagtcg ccaggccctc gctgaagacg gtcaaatacg
     3661 ccttggaggc gcagtagttg acgctgccgg gactaaacag aaatccagcg acggaggaga
     3721 cgttgatcac tcctcccgat cgacgcgcca gcatgcccgg gagcgcagct tgaatcagcc
     3781 gcatcggagc gagcacatgc aggtgcaaca tcgcgacctg gggctcgacc ggcgtttcga
     3841 tcagaagacc cttggttccg aagccagcat tgttcaccag cagtgagagc ggattccccg
     3901 ttttgattcg ctcggccacg cgcgcaacgt cgtcatcccg cgagaggtcg gcctcgagga
     3961 cctcgaccga aatccccagg ccggatagct cccgggctac tgccgcaagc cgctgtccgt
     4021 cccgggagac gagcagaaga tcgtaaccgc gccgcgccag ccggtcggcg aagacccggc
     4081 cgatgccggc ggtcgccccg gtgaccaaag cgagcggacg ggcgcctccg gagggggtcc
     4141 tcatggtcgt gcaatctgcg cggcgccatg gcggggccca ttttcagatt ctgcactccg
     4201 agccatcggg gccgccgggc tcacaccgtg ggcgtggtcg atcccggcca ggtcggcccc
     4261 gcccacgccc ctgcaaccaa agcacttaac aatcaccgaa aagacctcga aggcggactg
     4321 ccggcgcccg cggtgaacag aatggcgtgc aatgtgctgc atgcgggcca acagcatgat
     4381 tttcgcgtag ttgccgccga tgctcgcgcc ccacccgggt cgcgacgaat ccggagctct
     4441 caaatgtaca gaatcgagtt cgagcccggc gatgtcgggg tgttcaggag tgtcgaagag
     4501 gtggccaccg ccatcaagag cggcgtgatt acctcgcgtg cgcgcatcta ccaccaggcc
     4561 accgacaagt ggctgccgat cgaatttcat ccgcactaca agaaagcgct cgagatggtg
     4621 gcgagaggca acgttccagc cccgtccagc agcacgcgtt tcaacggctc gccgttcacc
     4681 ccaccgggcg ctcgcgcagc gatacaggtg gcgccaccgg ccccggcagt tgtgtcccca
     4741 cccgcaccaa tagttgtcga agctgccaac gaaacgccgc cgacgatcaa ccacgtcgag
     4801 ttggagcctc cggccgcgac gcacttcgag ccggagccag ccgctgaggt tccgcttcct
     4861 ccggtcgagc gggaacccgc cccggaaccg gccgtggaaa tgagcaatcc cgcgatcgtt
     4921 gaacccgaac cggccccgcc tcctcccaat ttcgtcttca tcccgcgatc gagggagatt
     4981 cggttcattc cgctggaaga gcccaagccg ttggggcgct cacgtcaaag gcacctggaa
     5041 tatccgattc cgccggctgc gctggcgcct gagatgcccc cgttcttcgt gggtgccccg
     5101 agcgacgagc tgcgatacca ggaggtggca ccggaagtcc cccaaagtga acatccgggc
     5161 aacgagagcc tggcgatcga caccgcgggc atggacagcc tggctctcgc ggcggatgcc
     5221 tcgcctcggc cgatattccg accgagattc cgcgtgggat cgctgggaaa gctgcggcgg
     5281 ccgatgttga tcggtgtcgg agccttggcg ttgatcgtct cgacacgctt cggcatctcc
     5341 gcggccgcga gcaccctcaa cgccggcgtt atgacctccg gctggggtgc gctccccttc
     5401 ctcaaccgcg agtcgggcgc cgaccgctcc ggcgaatcgc tcaaccaggc tcaagccggt
     5461 ggccggctcc cccggaatcc cgaaccccag ggcagtccga gcttcggctc gagcagcgcg
     5521 tttccgatcg ggcgctcggg ttcgggggat tccagacggc agcccggagg agtgaactcg
     5581 gcgaccagcc ccggcgagaa gatagagcaa gtcgctacgc ccgactctat ggtccgaaag
     5641 gcgccggtgg cacagatcgc catcgcggcg cccaagatcg ccggactgcc gggcgcgacc
     5701 aacgtcaagc tgactcccgc agtggtggtc acgcactatg aagccgccta cgcggctgcg
     5761 cgatcggagc tcgagaccgg gttccgcacc gccggtttcg ccaatgtgtt cgcgcccgag
     5821 cacttgaact cggcgcaagg cgtccgggcc gcgcgcctgg ccgccggtac agcttcagcc
     5881 tacgtcgcca agtaccggcg ccgggaagct gaaatcgagg cggcctacgc cgactctgcc
     5941 gccgagctgg ccaagacccc ggctgaccgg aaggtctggg atgggcgcaa agtgctccag
     6001 gaaagccccg aagtcgccaa gctggccgga tttctcctgc aggagatcga cagcgttttc
     6061 ggtgtgttgg cgtcgcagga tggggcgtac gagatcaagg acggggcgat cgtgttccag
     6121 gacgcggccg ccgcgcgagc ctacgccgag cttcgtccgt ggctcgaacg tccggcccac
     6181 cagtgggctg actctgcgga aggcacaccc aactcggcag ggcgcgttct ccgtgccatc
     6241 gggtccacca ggcttcccga aggtggcgcg ttctaacgat cgcttagcgc tggatcagtg
     6301 agaaacccgg aaggccggcg ccattgcccg ttccagctcg gcgtccgagg cgtccggatt
     6361 cttgcgataa aggtctatca gaccggcgac gtttcgtgac gagcgccaga gctcctcttc
     6421 ggttcccggc gcacgatctt cgtcacccga ccgacgcgcg aacatttcgc tgaagagcag
     6481 cctcgcctgc cgcaggaact tcatctttcg tcccgtttcc ctgatcacta cagagaccgc
     6541 cgagtctatc acggtgcggc ccccgccggg gttcgccgct aatgcctccc ccacgacggt
     6601 ctggacgttt cgataaaaga gcttgcgttg cggctcggtg agactgagcg tctcggccgc
     6661 ggcgagacag gtggagtcac gctcggcgtc aaaagcggcg gtgtcggcca cgctggtggt
     6721 agggacgggc tgggacggcg agtcggcgga ttgagcgcgg cctgcgctaa cggccacgga
     6781 caacaacaac agccccgccg acgagccgag gaatgtgcgc gtcatctcgg cctccggaac
     6841 ggagcggagc tacatcattt acgataaagc tttttgtatg ggacgcgagg gttcgccccc
     6901 acccaggact agtgacccag gcggctcacg cgaaagccgc ggtgcccccg aggcattact
     6961 tcgccccgtc cggggatgca ccggtcatcg ccaatgacga tccagggccg gcgaatctga
     7021 tcttcggtgg ctacgtcgct ggcggggagc ggctgcgtgc cagttccccc cgaaacggga
     7081 agaacggtca ccgtctgacc ggggtcgagc ggcatgcctg cccctccggc gttggcgacg
     7141 tcgcgcggtc cgggggccgg cgattgcgga gccggctccg ggtccggtgc cgttaccggc
     7201 gcatccttgg cggcccggtc cacctgcttc gcatgccggg cgtggacccg ggcagggacc
     7261 ggcgtcagcg cgctcgcccc ggcagcgttc ttctgctcgg gctcggcccg cggcaggtcg
     7321 agctcccggg cgctcgcgac cgcatcggtg gccgattccg ccgagggaag cagggtcagg
     7381 tcccgaggtg tcgaatcgga acgctcggag gagcaggccg caactaccag tgctaggggg
     7441 atggcgaggc aggtatagcg agtgtgcatg gcccagaggc ctccgaacgg caggaacgga
     7501 actgagtgag aatactacaa agacgatggc aaggaaagac cgcgcacaaa acagcgtgaa
     7561 cggtaaacgg ggggctccgc gtcaccggga cccccccgtt tactgttcac cgttcacgct
     7621 gttacgtcgt accagaccag tcggctattt cgccctttct aagtaaaccc cttcccgcgg
     7681 attcactcgg actcgttcac cctcgccaat gaattccggc acctggatgg tgacaccgtt
     7741 gctgagcttg gccggtttgg tgctcgccgt cttggttgct gtcttcatga tgggcgccgt
     7801 ttcgacgatt tccatctcga ccgccgacgg cagctcgatc gcgatggggc gtccgttcag
     7861 ccactcggcg acgaaggtca tgccggattc cagccaggat ccggcatctc ccacgacctc
     7921 gtcatcgagg gagtactgct cgtacgtgcc gttgtccatg acgtgcacgc cgttcgcgtc
     7981 gcgatagaga acctgcagct ctttggtatc cagccgggcc tcatcgacga actcggttgc
     8041 gctcaggcgc gtatcgaacg tgttccccga attcagattg cgaagccgca cctgcacgaa
     8101 tgcacgcaag ttgcccggtg tgcggtgctg aaaatccata acgcggcacg gcgccccatc
     8161 gatgattagg acgtgaccgc gacggatgtc ggatgccttc atctctactc ctccaccaat
     8221 gaagccggct cccgggtcgg ggcgcgggcg caggaagata aaccagcggc ctaatggggc
     8281 aactcgcggt cgcagcctgt gggaggggct gagacggcct ggggccggcg gggttcttga
     8341 ccgcacgccg ctggccttcg catatatggc ggcaaaccga gagcccggag gagcgagcct
     8401 ctcatgagtg cgcgaaacga gtctgaactc ggcgccgacg aacgcatttg ggaagacgta
     8461 cacgagcggt tgatgggcca ccccgacttc gacgccaccg acgtcgagat cgaagtggag
     8521 gggggactgg tgaccctggc cggcagggtg gacagtcggg aaacgaagtg gttggcagaa
     8581 gagatcaccc gggccgtaca aggcgtccag gacgtacaca accgcctcaa ggtggcccgc
     8641 tggtaggcta gccctcggcg cttctcgcca agcctgccaa cgtttcgagt gcctgctcca
     8701 gctcgccgcg gagcgggaat ccgcagttga gccggaaaca gtgcccgaaa gtgtcccctg
     8761 tcgaaaagac ccgtccggga acgatcccga tgcgacgggc caacgcgctc cgcgccagcg
     8821 caagcgtgtc cgttcttggt gcgagctcga tccagagaag gtgaccgccc ttgggccggg
     8881 agacccgagt cccttgcggg aacagcctcg cgatcgcgtg ggcgtagtcg gagacctgag
     8941 cccggaatgc ctggcgcagg cggcgaaggt ggtgctcggc cccgcccccg cttagatact
     9001 ccgccacggt cagctgtgcc aaggtgttgg tatggattga gcttgccagc ttgagctggc
     9061 gaattcgtcc ggcgtgacga gccgcagcga cccagccgat gcggtagcct ggcgccagcg
     9121 tcttggagaa ggaagagcac agaatcacgt tgccggtagt gtcgaaggcc ttgagcggcg
     9181 ccggccgtgt ctccccgaag tacaagtccc cgtagagatc gtcttccacc accgcgacat
     9241 cgtgtcgcgc gagcagcgcc acctgctccc gcttgactgc gtccggcact gcaaacccca
     9301 gcgggttctg gaaattggga ttggtgagga atacccgcgg cttggtgcgc cgaatggccg
     9361 agtcgaggtg ctcgagggac gctcctgtct ccggatcggt tggcacttcc actacccgca
     9421 gatccagtga ctccgccgtt tgaggaaatc cgtagtacga aggtgattcg accaggatca
     9481 cgtctcccgg tcgcgtaagc gcccgcaggg caagctgaat cgcctcggtg cagccgttgg
     9541 taacgatcag ctcgtccgga cggagcgtga gcccggcggc aagagcccgc cgagctaatt
     9601 gatggaggag agcctcatgg cccactggag acccgtagac cagaccggcg gctcccgctt
     9661 ctcggatgac tcgcgtcatg atccggccca gccgatgcgt cgggtagtgc tctggtgcgg
     9721 ccgtcgcgat tccgaagggc gccatgccct tgtcgttcac ccgggccagc agctcccacg
     9781 cgagatccgc gatccgcacc tcttccttgg tgaacgacgg cctcgggatg gagggcgacg
     9841 gcgcccgact caccggagcc gcgctcacgt agtgtcccga ttgaggtcgg acctcgaggt
     9901 acccgcgatc ctcgagcagc tggtaggcgc gcaacacggt cgcgaggctc acccgtttct
     9961 gccggctgta ggtgcggacc gaggggagcc gatcgccgct tcggagggtg cgggtttcga
    10021 tcaggcctac gagctcatcg gcgagccgtt gatagagaac gcgctcaccc cgtcggcggc
    10081 gcctgggaac tgtactggtt gcagtcgtca tatgctgaaa ctgttatggt tacagattcc
    10141 acgatacgtt gagacgtgcc gtccggcaag tcaccgtccc cggagctcgc gatgctcgat
    10201 cccacggtcc tgcttgacac ccgcatcgtt ccctacggcc ccgagcaccg aaacgccttc
    10261 cgcgatctca acctcgaatg gatcgcgacc tacttcgaag tcgaagccga ggatcggaaa
    10321 atcctgggcg accccgaaac ccatgtcctg ggcaagggcg gcgcgatcct gatggcgctc
    10381 gagggcgagg agccggtggg aaccggcgcc ctcatcccga ctgggagcca tgagttcgag
    10441 ctggccaaga tggcggtcac tccccgcgct caggggcggg gtgtgggccg gcggctctgc
    10501 gctgcgctga tcgatctggc ccgacggatg ggcgctcacc ggatcgaact ggtctctcac
    10561 cgatcgctgg ttcccgcgat tgcgctgtat cgatcgctca gcttccgtga agtaccgctc
    10621 ggcgacgtcg cctatcgtcg cgccaacatc cggatggagt tgcggctccg gtgagggccc
    10681 gcatcatcgc ggccttcgcg gtcgtctacc tcgtctgggg ctcgacctac ctcggcatcc
    10741 gctgggcggt cgagtcgttc cctcccttcg tgatgacggc aagccggttc ctggtggccg
    10801 gcgcgttgct gtacctgctc gcccgcctgc ggggcgcgcc ggcgccgccg cggcgggctt
    10861 ggctcgcggc gatcgtgata ggcacgctgc tggtcaccgg
//
LOCUS       DASRJQ010000188         6889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_718341, whole genome shotgun sequence.
ACCESSION   DASRJQ010000188 DASRJQ010000000
VERSION     DASRJQ010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6889
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718341"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..370)
                     /locus_tag="VFB89_04315"
     CDS             complement(86..370)
                     /locus_tag="VFB89_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26554.1"
                     /translation="MGYALMMGGNPDPFRVREPPPTAGHPRFDGKEATLTIGFMGTEW
                     LGAQGGSAAALAGFLLLAAMLLHVRRANRLERSRARRQLRREARTQRHHR"
     regulatory      534..630
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            661..2364
                     /gene="thiC"
                     /locus_tag="VFB89_04320"
     CDS             661..2364
                     /gene="thiC"
                     /locus_tag="VFB89_04320"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF006763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HZM26555.1"
                     /translation="MATSITSASTAGSAYGEAFPRSTKVYLEGSRGVRVPVREIALSD
                     GEPPLQVYDTSGPDGFDVHEGLPPLRRPWILARNVDRVEQRAEGRHDGHRDREPVPAG
                     LRRTPYRGRAAVTQLHYARLGEITPEMEFVALREGLPSELVRAEVANGRAIIPANINH
                     PELEPMIIGRKFLVKINANIGNSIVTSSVAEEVDKLRWATLWGADTVMDLSTGRDIHA
                     TREWILRNSAVPIGTVPIYQALEKVGGVAEDLTWDVYRDTLIEQCEQGVDYFTVHAGV
                     LLEYVPLTANRVTGIVSRGGSILAKWCLAHHRQNFLYARFREICEILAQYDVSFSLGD
                     GLRPGSIADANDEAQFAELRTQGELTRIAWEHEVQVMNEGPGHVPMHLIRDNMDKQLE
                     WCDEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPDRDDV
                     KAGVIAYKIAAHAADLAKGHPRAREWDDALSRARFDFRWEDQFNLALDPVTARAFHDE
                     TLPAEGAKLAHFCSMCGPKFCSMKLTQDLREEARRQAEAGMAEKAAEFRARGGEIYLR
                     K"
     gene            2374..3339
                     /locus_tag="VFB89_04325"
     CDS             2374..3339
                     /locus_tag="VFB89_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26556.1"
                     /translation="MRGEKEARLAGLGALVVLAALGCRSDRPAAAPLTTAALSQDSAR
                     PQVPHRLREVTGLSHPEAARYDPELDLWFVANINGDPLAKDNNGFITRLKSDGSVGVL
                     KFAEGGRNGVRLNGPKGMAIVGDTLWVADIDAVRGFNRRTGAEIASVSLSGQAKFLND
                     IAAGPGGIYVTDTGIGPDGKGGVKHTGPDRVFKIDPSRKVSIALQSDSLQGPNGITWD
                     SAGRRFVIVPFFGKTPLSWSPGDAAPHAIGKGPGQEDGVEAIGPNHYLITSWADSSLF
                     ALEQGQATRIAGPLPSPADIGWDARRHRVAVPLLLEDRVEIWELP"
     gene            3336..4172
                     /locus_tag="VFB89_04330"
     CDS             3336..4172
                     /locus_tag="VFB89_04330"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZM26557.1"
                     /translation="MRPTSRRRNGAASHVLIAVIGVAAGMGMAWGLGDRHLRDAVTTH
                     GEWVYLKKRGQTDSVRAYIAYPERKDRAPGVVVIHEVFGLTDWEPTVVDRLAKNGYVA
                     IVPDLLSTKYGRSPADPDSGRKLVAQLEPSRITADLDAAVEYLNAQSAVQSGIIGIMG
                     FCWGGGQTFRYTTNNPKLKAAVICYGPPPAHDALARIETPLLGVYGENDARIDASLPD
                     VAARLKGMGKSFTYQIYPGTGHGFLKPGREGSDGPQVEKAWTNILNFFKKELQPKTRT
                     ES"
     gene            4172..5773
                     /locus_tag="VFB89_04335"
     CDS             4172..5773
                     /locus_tag="VFB89_04335"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HZM26558.1"
                     /translation="MILPFLLLQTTAASPLAVRPDTARPLHDARHYAITLVLGDSGHH
                     VLGEIETSWRLRSSEPIQLELDSALRVVRVLVNGRENTRLARTMYGRGANDVVIPHDG
                     KAGDSLTTRIRYHGDVRLGLAFRGDKTHNLAIFGDNWPDRAHGWLALQDFPNDKATVA
                     FKVQVPVGYQVLANGILEKVDTLPYSHIVWTYRMDRPIPPYTMVIAVGKFAVTRLPDA
                     ACSVRCVPQTLWTFPEDSAFAVAGPFRRVGSIVDFFSRLIGPFPYVGLAHVEAVTPYG
                     GMENATAIFYNDSLYRARSLPEETVAHETAHQWFGDAVTEDDWHHLWLSEGFATYLAA
                     MWAEQAGGAAALKTAMQNNAEAYFKSRAVDRPILDPAVRNLDSLLNENNYQKGAWVLH
                     QLRGMIGDSVFVSGLRTYYQRFRDSTALSLDFAKIMSEAAGRDLDWYFRQALTQPGYP
                     MLAVSTSREGGKLVVEIRQTQKSEWGTYRLPGLEVLLDGKLVRMDVDAPTQRTVFNGF
                     SKIPSKVEIDPNGRWLLRRSVNGER"
     gene            5777..6631
                     /locus_tag="VFB89_04340"
     CDS             5777..6631
                     /locus_tag="VFB89_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26559.1"
                     /translation="MNGELRTVHAAHTPRRSRSFPFTVYRSPFTLFKPFSLLSVLLPA
                     CGGLSPLRGHAVVGRDAYLLFVGETANGQSDLFGVRADGGPVFQITYTPVVEATPVLS
                     PDGGEVAFLRGATIGDKKPGRVWVLNLLSGAERELQLPRGVAAPNALGWSEAGTSIYV
                     RTDSTVYRLVAPPGSAEPRITTDGERARAESSLAVLVGQPPFGSVVPCGSDLCVQSEQ
                     SAPEPFASNAHDPIRWGPDSVGFVSGPDLIVRPVGPGHPRRVEWSGAPSGVRGLTFFA
                     GKSVNGER"
     gene            complement(6683..>6889)
                     /locus_tag="VFB89_04345"
     CDS             complement(6683..>6889)
                     /locus_tag="VFB89_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26560.1"
                     /translation="RQVRDALQQIIASGKADATVRTGPAELWAGVWLAVVGWAAERVI
                     VKEWNPGHPQAIQAVDAAWDAIKA"
BASE COUNT         1243 a         2184 c         2241 g         1221 t
ORIGIN      
        1 tactcgacta ccccaaaaaa ccgccgcccc caccatctcg gctcgagacg cgggggcggt
       61 cggcgttgcg caactagctc gcgctttacc ggtggtgccg ctgcgttcgg gcctctctcc
      121 tcaactgccg gcgcgcccgg ctgcgttcga gccggttggc gcgccgcacg tgcaacagca
      181 tcgctgccag caggagaaaa ccggccagag cagccgcact tcctccttga gcgccgagcc
      241 attccgtacc catgaatccg atcgtcaacg tcgcttcctt cccgtcgaaa cgcgggtgac
      301 ccgcggttgg cgggggctcc cggaccctga atggatcggg gtttccgccc atcatgaggg
      361 catatcccat gcccgccctg ccggggggcc ctaacgcctc tcaagccgcc gaataacaga
      421 gggttggccc gctggtgccg gcgggtgatc ctcgcgatct gcggcagatt caaccgacgg
      481 aagccgcgcc ggctggttga tttgaccgtc ctccggcagg ccgcttacgt tggaacagcc
      541 gccaggggta tcggtgaaaa ccgattgaga gagaaccctt ggaacctgat ccggttcata
      601 ccggcgaagg gagcgcggcg catttgcgcg gcgcccttcg tcttcgttgg gagcgtcgcc
      661 atggccacgt cgattacctc tgcctccacc gccggctccg catacggcga ggctttcccc
      721 cgatccacca aggtctacct cgaaggaagc cgtggcgttc gagttccggt gcgggagatc
      781 gcgctctcgg acggcgagcc accgcttcag gtctacgaca cgagtggacc ggacgggttc
      841 gacgtccacg aggggcttcc gccgctgcgg cgcccgtgga ttctcgcccg caacgtcgac
      901 agggtcgagc aacgggccga gggacgacac gacggccatc gcgatcggga gcccgtgccc
      961 gcaggccttc ggcgcacgcc ctatcgcgga cgtgccgccg tgacgcagct ccactacgcg
     1021 cgacttggcg agatcactcc ggagatggaa ttcgtcgccc tgcgggaagg cctgccgtcc
     1081 gagctggtcc gggccgaggt ggccaacggc cgggcaatta ttcctgccaa catcaatcac
     1141 cccgagctcg agccgatgat catcggccgc aagttcctgg taaaaatcaa cgcgaatatc
     1201 gggaactcga tcgtgacatc atcggttgcg gaggaagtcg ataagctgcg ctgggccacg
     1261 ttgtggggcg cagacaccgt gatggatctc tccaccggca gggacatcca cgccacccgc
     1321 gaatggattc tccgcaactc ggcagtgccg atcggcaccg tgcccattta ccaggcactc
     1381 gagaaggtgg gcggtgtggc cgaggacctg acttgggatg tctaccgcga cacgctgatc
     1441 gagcaatgcg agcagggtgt cgactacttc accgtccatg cgggcgtgct gctcgagtat
     1501 gtgccgctca ccgccaaccg tgtgacgggt atcgtttccc gaggcggatc gattctggca
     1561 aagtggtgtc tcgcgcatca ccggcagaac ttcctctacg cccgcttccg ggagatctgt
     1621 gagatcctcg cgcagtacga cgtctccttc tcgctcggcg acggacttag gcccggcagc
     1681 atcgcggacg cgaacgacga agcccagttt gccgagcttc gcacccaggg agagctcacc
     1741 cgcatcgcct gggagcacga ggtacaggtg atgaacgaag ggcctggcca cgtgccgatg
     1801 catctgattc gcgacaacat ggacaagcag ctcgagtggt gcgatgaggc gccattctac
     1861 acattgggtc ccctcaccac cgacatcgcg ccggggtacg atcacatcac gagtgccatc
     1921 ggcgccgcga tgatcggctg gtacggcacc gcgatgcttt gctacgtcac gcccaaggaa
     1981 cacctcgggc tgcccgatcg cgatgacgtc aaggcaggtg tgatcgccta caagatcgcc
     2041 gcccacgccg cggatctcgc caagggacac ccgcgagccc gcgagtggga cgacgccttg
     2101 tcgagggcgc gatttgattt ccgctgggaa gaccagttca acctggcgct cgacccggta
     2161 acggcccggg cctttcacga cgagaccctg cccgccgagg gcgccaagct cgctcatttc
     2221 tgctcgatgt gtggtcccaa gttctgctcg atgaagctca ctcaggattt gcgtgaggag
     2281 gcgcgtcgcc aggccgaggc cggcatggcc gagaaggcag ccgagttccg ggcgcgcggc
     2341 ggcgagatat acctgagaaa gtgagggtga caggtgagag gcgaaaaaga agccagattg
     2401 gcgggactgg gcgcattggt ggtgctcgcg gcgctgggat gccggtcgga ccggccggcg
     2461 gccgccccgc tgaccaccgc ggcgctcagt caggactcgg cgcgaccgca ggtcccccac
     2521 cgcctgcgcg aggtgaccgg tctctcccat ccggaggcgg cccgatacga tcccgagctc
     2581 gacctgtggt tcgtcgccaa catcaatggg gatccgctgg ccaaggacaa caatggcttc
     2641 atcacgcgac tcaagagcga cggctcggtc ggcgtgctca agttcgcgga gggtggccgc
     2701 aacggagtcc gcctgaacgg tccgaaagga atggcgatcg taggcgacac gctctgggtg
     2761 gccgacatcg acgctgtgcg cggattcaat cggcgaaccg gcgcagagat cgccagcgtg
     2821 agcctgtccg gccaggccaa gttcctgaat gacatcgcgg cgggtcccgg cgggatctac
     2881 gtgaccgata ctggcatcgg ccccgatgga aagggaggag tgaagcacac cggcccagac
     2941 cgcgtcttca agatcgaccc ttcccgcaaa gtcagcatcg cgctccaaag cgatagcctg
     3001 caaggtccca acgggattac ctgggactcg gcgggccgtc gcttcgtgat cgttccgttc
     3061 tttgggaaga cccctctgtc ctggtctccc ggtgatgccg ctccccacgc gatcggcaag
     3121 ggtcccgggc aggaggatgg cgtcgaggcc atcggaccca atcactatct cattacctcc
     3181 tgggccgact cgagcctgtt cgcgctggag caggggcagg ccacccggat cgccggcccg
     3241 ctcccctcgc cggccgacat cggttgggac gcgcgcaggc atcgggtcgc tgtgccgctg
     3301 ttgctcgagg accgggtgga aatctgggag ctgccatgag gccgacatcg aggagacgca
     3361 acggcgcagc gtcacacgtt ctgatcgccg tgatcggcgt ggctgcaggg atgggaatgg
     3421 cgtggggttt gggtgatcgc cacctgcgtg atgccgtcac cacccacggc gaatgggtgt
     3481 acctgaagaa aaggggacag acggattcgg tccgagcata catcgcgtac cctgagcgaa
     3541 aggaccgcgc gcccggtgtg gtcgtcattc acgaggtatt cggcctgacg gactgggagc
     3601 cgaccgtggt cgacaggctg gccaagaacg gctacgtcgc catcgtgccg gacctgctct
     3661 cgaccaagta cggtcgttct ccggccgacc ctgactccgg gcggaagctg gtggcgcaac
     3721 tcgagccgtc gcgcatcacc gccgatctcg atgctgcggt ggagtatctc aatgcgcagt
     3781 cagcggttca atcggggatc atcggcatca tgggcttttg ttggggaggc gggcagacgt
     3841 ttcgctacac gaccaacaat cccaagctga aagcggccgt gatctgctac ggcccgccgc
     3901 cggcgcatga cgcgttggcc cggatcgaaa caccgctcct cggggtctat ggggagaatg
     3961 acgcccggat cgacgcttca ctgccggatg tcgccgcgcg cctcaagggc atgggcaagt
     4021 ccttcacgta tcagatttac cccggtaccg gccatggctt cctcaagccc gggcgagagg
     4081 ggtccgatgg accgcaggtg gagaaggcgt ggaccaacat cctgaatttc ttcaagaagg
     4141 agctgcagcc taagacccga actgagtcct gatgatcctt ccgtttctgc tgcttcaaac
     4201 aactgccgcg tcgcctctgg ccgtcaggcc ggatacggcg cggcctctcc acgacgccag
     4261 acactacgcg atcactctgg tgctgggcga cagcggccat cacgttctcg gagagatcga
     4321 gacgagctgg agactgcggt cgtcggagcc gatccagctc gagctcgatt cggcgctccg
     4381 ggtggtccgc gtgctggtga acggaagaga gaacacccgc ctggcgcgca ccatgtacgg
     4441 ccgcggtgcc aatgatgtgg tcattcctca cgacgggaag gcgggtgact cgctcaccac
     4501 ccgcattcgc taccatggcg atgtgcgtct ggggctcgcc tttcggggcg acaagactca
     4561 caatctcgcg atcttcggag acaactggcc ggaccgggcc cacggctggc tcgcgttgca
     4621 ggactttccc aacgataagg cgacggtggc gttcaaggtc caggtgccgg tcggttacca
     4681 agttctcgcg aacggcatat tggaaaaggt cgacacgctg ccgtactcgc acatcgtctg
     4741 gacctatcgg atggatcgcc cgatccctcc gtataccatg gtgattgccg tggggaagtt
     4801 cgccgtgacg cgactaccag atgcggcgtg ctcggtccgt tgtgtccctc agacgctgtg
     4861 gaccttcccg gaagattcgg cctttgcggt cgctggaccg ttccgccgtg tgggcagcat
     4921 cgtcgatttc ttctcccgcc tgatcggccc gttcccttac gtagggctgg cgcacgtcga
     4981 ggcggtgacg ccatacggcg gcatggagaa cgcgaccgcc attttctaca acgacagcct
     5041 ataccgcgcg aggtctctgc ccgaggagac agtcgctcac gagaccgcgc accagtggtt
     5101 cggcgatgca gtgacggaag acgactggca tcacctctgg ctgtcggagg gctttgccac
     5161 ctatctcgca gcaatgtggg ccgagcaagc cgggggggcg gcggcattga agacagccat
     5221 gcagaacaat gctgaggcat acttcaagtc ccgcgcggtc gaccgcccca tcctggatcc
     5281 cgccgttcgc aacctcgatt ccctgctcaa cgaaaacaac taccagaagg gcgcctgggt
     5341 cctgcaccag cttcgtggga tgatcgggga ttcggtattc gtgtccggat tgcggaccta
     5401 ctaccagcgt ttccgggaca gcacggcgct gtcccttgat tttgcgaaga tcatgtccga
     5461 ggccgccggt cgagacctgg actggtactt ccgacaggcg ctcactcagc cgggctaccc
     5521 gatgctggcc gtcagcacca gccgggaagg gggcaagctg gtggtcgaga tacggcagac
     5581 ccagaagagc gagtggggta cctatcgtct gccggggctc gaggtgctgc tcgacgggaa
     5641 gctggtccgg atggacgttg acgcgccgac ccagcggacg gtgttcaacg gcttctcgaa
     5701 gatcccctcg aaggtcgaaa tcgatccgaa cggacggtgg ctgctcagaa gaagcgtgaa
     5761 cggtgaacgg taaacggtga acggggaact ccggacagtg catgcggcac atacaccgcg
     5821 ccgatcccga tcattcccgt ttaccgttta ccgttcaccg ttcacgctgt tcaagccgtt
     5881 ctcactcctc tccgtactat tacccgcctg cggcggcctg tctcccctgc gtggccacgc
     5941 cgtggtgggg cgtgatgcct acctgctctt tgtgggcgag acggcgaacg gacaatccga
     6001 cctcttcggt gttcgggcgg atggcggtcc ggtctttcag atcacctaca cgccagtggt
     6061 cgaagccacg cctgtgcttt caccggatgg aggcgaagtg gcgttcctgc ggggagccac
     6121 gatcggcgac aagaaacctg gaagggtctg ggtgctcaat ctcctgagcg gagccgagcg
     6181 ggagcttcag ttgccgcgag gtgtagccgc gcccaatgct ctcggctggt ctgaggctgg
     6241 aacgtccatc tacgtgcgaa ccgacagcac ggtctatcgg ttggtcgcac cacccggatc
     6301 ggccgagccg cggataacga cggacggtga acgggcgaga gcagaatcga gccttgcagt
     6361 gctggtgggg cagcccccgt tcggaagtgt ggttccatgc gggtcggatc tttgcgtcca
     6421 gagcgagcag agtgctcctg agccgttcgc gtccaatgct cacgatccca tacggtgggg
     6481 gccggactcg gtggggtttg tgagcggacc ggaccttatt gtgcggccag tgggcccagg
     6541 acatccgcgc cgggtggagt ggagcggtgc gccgtcagga gtgcgggggc taacgttctt
     6601 cgcagggaaa agcgtaaacg gtgaacggta aagaggagac tacgccgagc ctttgacccc
     6661 attcactgtt acagttcacc gtttacgcct ttatcgcatc ccacgcggcg tcgaccgcct
     6721 gtatggcctg gggatgacca gggttccact ccttcacgat cactcgctca gccgcccagc
     6781 cgacgacggc aagccagact ccggcccaca gctcggctgg tccggtgcgg accgtggcgt
     6841 ccgccttgcc ggaggcgatg atctgctgga gcgcgtcgcg cacctggcg
//
LOCUS       DASRJQ010000189         3038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1368344, whole genome shotgun sequence.
ACCESSION   DASRJQ010000189 DASRJQ010000000
VERSION     DASRJQ010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368344"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="VFB89_04350"
     CDS             complement(<1..794)
                     /locus_tag="VFB89_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HZM26561.1"
                     /translation="MSAVSLPPAEELAGWRYPRTQPSLPESNATVSVPRKVHWWRKAL
                     AFAGPGYLVAVGYMDPGNWATDLAGGARFGYALLSVILLSNLMAILLQALALRLGIVT
                     GRDLAQACRDHYSRPVSFGLWVLCEIAIAACDLAEVIGSAIALNLLFGIPLVWGVCLT
                     ALDVLLILLLQNRGFRYLEAFVLALVFTIGACFAIELILARPDVAAVAQGLVPTTQIV
                     TNPAMLYIAIGILGATVMPHNLYLHSSIVQTRRIGSDVGSKREAIR"
     gene            complement(791..1477)
                     /locus_tag="VFB89_04355"
     CDS             complement(791..1477)
                     /locus_tag="VFB89_04355"
                     /inference="COORDINATES: protein motif:HMM:NF014762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HZM26562.1"
                     /translation="MNTAGLKEPLTASVEDYLKAIYSLSPEGRAASTSDIAHRLELSA
                     PSVSGMVKRLSEQGLLEHVPYKGVQLTPEGRRAALRMIRRHRIIETYLVDRLGYTWDT
                     VHAEAERLEHAVSDALIQRMADALGNPHADPHGEPIPTADGLIEEVSATPLPEVPLGE
                     TVEIRRVTAGDPERLRYLASLDLMPGATVTVVERQPFQGPLTLRVNDRLQVLGNELGS
                     SLLCTRSVPR"
     gene            complement(1603..2196)
                     /locus_tag="VFB89_04360"
     CDS             complement(1603..2196)
                     /locus_tag="VFB89_04360"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HZM26563.1"
                     /translation="MPLPQPLNQVPPSFARLAQQLAGHKPIWTNDPERTWAAVALILA
                     PDPDSLLLIRRAERVNDPWSGHVGLPGGRQESGDSSLLQTAIRETAEEIGLTLSDAQS
                     LGALDDVVPRTVSLPPIAVRPFVFALDQRPLLVLSDEIAAVHWIPLRQLRDPATHAPT
                     VVLVGGESLVVPAYVVGDLVVWGMTERILSCFFQVSQ"
     gene            2480..>3038
                     /locus_tag="VFB89_04365"
     CDS             2480..>3038
                     /locus_tag="VFB89_04365"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZM26564.1"
                     /translation="MATATLTKHRLSGALGEILIDVRSGGRSSPRPAVVVVHGFKGFK
                     DWGMFPPLAERLAQAGFTAVLPNLSGSGVDDSGEFTWPERFGHNTFSAELADVNSVLT
                     ALRAGNLGVAPPNGVGLVGHSRGGGICILLAAQDATIKALVTWAAISTVRRWPSEAEQ
                     AKWQAEGRLDVVNTRTGQVLPLYTDV"
BASE COUNT          615 a          919 c          994 g          510 t
ORIGIN      
        1 aaccggattg cctcccgctt ggagccgaca tcgcttccga tacgccgggt ctgcacgata
       61 gaggagtgca ggtagaggtt atgcggcatc accgtcgcac cgagaatgcc gatcgcgatg
      121 tagagcattg ccggattggt gactatctgc gtggtcggaa ccagaccctg cgccacggcc
      181 gcaacatcgg ggcgcgccag gatcagctcg atggcgaagc acgcgccgat cgtgaacacc
      241 agcgccagga cgaatgcttc gaggtagcgg aacccacggt tctggagcag aaggatcagc
      301 agtacgtcga gcgcagtgag acatacgccc caaaccagtg ggatcccgaa gagcagattg
      361 agtgcaatag ctgaaccgat tacctccgcc agatcacagg cggcgatggc gatctcacag
      421 agcacccata gaccgaagct caccggacgg gagtagtgat cccgacaggc ttgtgccaga
      481 tcgcgaccgg tgacgatccc gagtctcagc gcgagcgcct ggaggaggat cgccatcaga
      541 ttcgacagca ggatgacgct gaggagtgcg tagccgaatc gcgctccgcc ggccaggtcg
      601 gtggcccagt tgcccgggtc catgtagccg accgcgacga gatacccggg gccggcaaag
      661 gccaaagcct tgcgccacca gtgaaccttc cgagggaccg aaaccgtcgc gttcgactcg
      721 ggcaggctcg gctgagtgcg tggataccgc cacccggcga gctcttcggc tgggggcagg
      781 cttacagcgc tcatcgaggg acgctccggg tgcagagcag actcgacccc agctcatttc
      841 ccagcacctg cagccgatcg ttgacgcgaa gcgtgagagg accctgaaag ggctgccgct
      901 cgacgaccgt gactgtcgcc ccgggcatga ggtccagcga cgccaggtag cgcagccgct
      961 cggggtcgcc ggcagtcact cgacgaattt ccaccgtctc ccccagcgga acctcgggca
     1021 gcggtgttgc gctgacttcc tcaataaggc catcggctgt cgggatgggt tcgccatgag
     1081 gatccgcgtg cggatttccc agagcatctg ccatgcgctg gatcagggcg tccgacacgg
     1141 catgctcgag ccgctcggcc tcggcgtgga cggtatccca ggtatatccc aaccggtcca
     1201 ccaggtaggt ctcgatgatc cgatggcgac gaatcatccg aagcgctgcc cggcgcccct
     1261 cgggagtgag ctgaacgccc ttgtacggca catgttccag gagcccttgc tcagacaggc
     1321 gtttcaccat tccgctgacg gaaggggccg agagctccag ccggtgggcg atatcgctgg
     1381 tggaagccgc ccggccctcg ggtgagaggc tgtagatcgc cttcaaatag tcttcgaccg
     1441 aggcagtgag gggctctttg agaccggctg tgttcatgcg aacctggaca ttagtggcgg
     1501 ctaaaactgc tctgcgacta agctaatgtc aaggttatct ggtacaaaga agatggcgtc
     1561 tatatcaaga cgctatggcg acatttaact gttaagttac tgttactgcg atacttggaa
     1621 gaagcagcta agtatgcgct cagtcatccc ccagaccacc aaatctccga cgacataggc
     1681 aggaacgacc agcgattcac ccccgaccaa gactacggtc ggggcgtggg tcgccgggtc
     1741 gcggagctgc cgaagcggga tccagtggac ggcagcgatc tcatcgctca acaccagaag
     1801 tggccgctga tccagagcga agacaaaggg tcggacagca atcggcggaa gggaaacggt
     1861 ccgcggaacc acgtcgtcga gtgcccccag cgattgagca tcggacagag tgagcccaat
     1921 ctcttccgcg gtttcgcgga tcgcggtttg gagcaaactg gaatcacccg attcctggcg
     1981 tccccctggc aggccaacgt gcccggacca gggatcgtta acccgttcag ctcttcggat
     2041 cagcagcagg gaatcaggat caggcgcgag gatgagcgcg accgccgccc acgtccgctc
     2101 cgggtcattc gtccagatcg gcttgtgccc ggcgagctgc tgcgccagcc tggcgaagga
     2161 gggaggaacc tggttcagtg gctgtgggag tggcaagccc cgccgcaaca accgccccca
     2221 ggaggcgggc cgagtttgct gaagtcggcg gctttccccc ctgagccatg gcgagccggc
     2281 accgacatca gccgctcaac ctttcgaccg gagcacttgg ggcaggcaac cttggttccg
     2341 tggcggacca attgttcgaa gtcggtcccg caggagggac agtgatagtc gtaaatgggc
     2401 atgcgcgtaa gatagccgcc tcaccggagc tgccagcccc tggaggcgaa gcggtgaggt
     2461 cgcagatacc ttccctcgca tggccacggc aaccctgacc aaacaccgtc tttcgggagc
     2521 tctgggcgag atcctgatcg acgtccgaag cggtgggcgg agctccccgc ggccagcagt
     2581 cgtggtggtt cacggcttca aaggattcaa ggattggggg atgtttcctc cactggctga
     2641 gcgcctggcg caggcaggct tcacggcggt cttgcccaat ctcagtggca gcggcgtcga
     2701 cgattcgggt gaattcacct ggccggagcg cttcggccac aacacgttct cggccgagct
     2761 ggcggacgtg aatagcgtgt tgacggcact tcgggcgggt aacctgggcg tagcgccgcc
     2821 aaacggcgtg gggctagtag gtcactcacg gggcggcggg atttgtattc ttctggcagc
     2881 ccaggacgcc acgatcaagg cgctggtgac gtgggcggcg atatccacgg taaggcgatg
     2941 gcccagcgaa gccgagcagg cgaagtggca agcggagggt cggctcgacg tggtgaacac
     3001 ccgaaccggg caagtgcttc cactgtacac cgacgtgc
//
LOCUS       DASRJQ010000190         2626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1883762, whole genome shotgun sequence.
ACCESSION   DASRJQ010000190 DASRJQ010000000
VERSION     DASRJQ010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1883762"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VFB89_04370"
     CDS             complement(<1..410)
                     /locus_tag="VFB89_04370"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZM26565.1"
                     /translation="MQDAPPLIIGSGIAGLWTAWRLAAEGRAARLVTKEALADSASAW
                     AQGGIAVALGPGDNPLQHAADTLAAGDGLSDPAAVRVLTNEGPDRVRELLAMGAVFDR
                     APDGRLRYGLEAAHTRPRIIHAGGDRTGAALIALL"
     gene            complement(437..1519)
                     /locus_tag="VFB89_04375"
     CDS             complement(437..1519)
                     /locus_tag="VFB89_04375"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HZM26566.1"
                     /translation="MIGRLLDRYTLSLWLRLFGLTVLGFPIVSILINLTDSLNKLLDR
                     GLTVRQIIVSYVYSVPENMFLVLPAAVLFATVFTVGSMARHSELTAAKAGGQSFRRLM
                     LPIFLVAILATGLGIVVGEMAPGATAKQLKLQKAQPEGPTSARFNFVYRADQGWVYTI
                     RSLEIANRNLKTVLFERQGTGLRYPDLVVTADSATYDKQRRAWRLWNGTSRVIGSPER
                     QAAFTFRTLRLRTFSQTPADLLAEPKAPDEMRYAELGQYIEALQRSGNDANKLVVERA
                     LKLAIPATCLIIAIFGAPLAITSPRAGTAFGIAISLATTVLFLLLMQIAKAVGAGGLI
                     DPTAAAWLPNGVFLLIGVALLIRART"
     gene            complement(1516..>2626)
                     /locus_tag="VFB89_04380"
     CDS             complement(1516..>2626)
                     /locus_tag="VFB89_04380"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HZM26567.1"
                     /translation="GVVHQYKSDEADRFQVTRFATNEIRVRNVFDELERNTSDQIRGD
                     RELSSCAMLAVVRDADRDQDKAFADRRFFVERDLHELLALPTLQPDTTSPEPPGDYCL
                     WLDRLRTGVRARLAPPKLAAQAPDSHSTANVVGTTSAPSPPQPLSAVKQPKSLTTPAD
                     TPVTVAPKAPPATPAAPQRTAHPDVPARKLAPTRAVPQSKPAMKKLPASALVKSATDS
                     GPKPAPPLRLTSRAEVSLALDRAKDADLRADRYMVEVHKKWAISMACVSFVLIGLAMA
                     LRFPRGGMGLVIGGGLAVFSIYYVGLTAGEALSDRGLLSPALSMWLPNIILTVVGVLG
                     LIHVNRQPGLNRGGDFRELFDSLRYRFRWRRRRAA"
BASE COUNT          462 a          835 c          891 g          438 t
ORIGIN      
        1 agcagcgcaa tcaaagccgc gcccgtgcgg tcgccaccgg cgtggatgat ccgcgggcgg
       61 gtgtgggctg cctcgagccc atagcgcagc cggccatccg gagcgcgatc gaacaccgct
      121 cccatggcga gcagctcgcg cacccgatcc ggtccctcat tggtgagtac gcggaccgcg
      181 gccgggtcgg aaagcccgtc accggcggcc agagtatcgg ccgcatgctg caacggattg
      241 tccccgggac cgagcgcaac cgcgattccg ccctgcgccc aggcgctggc gctgtccgcc
      301 agcgcttcct tggtgactag gcgggcagct ctgccctccg ctgccagccg ccacgccgtc
      361 caaaggccag cgattcccga gccgatgatg agcggcggtg catcctgcat tacatgaata
      421 taaggcgcgc ggtgggctag gtcctcgccc gaatcagcag cgccactcca atgagcagga
      481 agaccccgtt gggaagccag gcggccgccg tcgggtcgat caggccgccg gcaccgaccg
      541 ccttggcgat ctgcatgagc agcagaaaca atacggttgt ggccaggctg atcgcgatcc
      601 cgaacgcggt tcccgctcgg ggcgatgtga tggcgagcgg agcgccgaag atcgcgatga
      661 tgaggcacgt ggctggaatc gcgagcttga gcgctcgttc gacaaccagc ttgttggcgt
      721 cattgccgga gcgctgcagc gcctcgatgt attgccccag ctccgcgtac cgcatctcgt
      781 ccggtgcttt gggctcggcg aggagatcgg ccggtgtctg gctgaaggtc cggagccgca
      841 gggttcggaa cgtgaatgcg gcttgccgct cgggtgaacc aatgacccgg ctggttccgt
      901 tccagagccg ccacgctcga cgctgcttgt cgtaggtcgc gctgtcggcg gtgacgacca
      961 ggtccggata gcggaggccg gtaccctgcc gctcgaacag taccgtcttg aggttgcggt
     1021 ttgcgatctc cagcgaacgg atggtgtaga cccagccctg gtccgcccgg tagacgaagt
     1081 tgaagcgtgc cgatgtcggg ccttccggtt gcgccttctg caacttgagc tgctttgccg
     1141 tggctcccgg cgccatctcg ccgaccacga tgccgagtcc ggtggcgagg atcgcaacca
     1201 gaaagatcgg cagcatgagg cgccggaagc tctggccccc ggccttggca gccgtgagct
     1261 cagagtgacg ggccatggag cccaccgtga acacggtggc gaagagcact gccgccggca
     1321 ggaccaggaa catgttctcc ggcactgagt acacgtagct cacgatgatc tgtcggacag
     1381 tgaggccgcg gtcgagcagc ttgttgagcg agtcggtaag gttgatcagg atggagacga
     1441 tcggaaagcc gagcactgtg agcccgaaaa gccgaagcca caggctcagc gtgtatcgat
     1501 cgagcagtct gccgatcatg cggccctgcg gcgccgccag cgaaagcgat accggaggct
     1561 gtcgaacagc tcacggaagt cacctccccg attcaatccg ggctggcggt tcacgtgaat
     1621 gaggccgaga acgccgacca ccgtcaggat gatgttgggc agccacatcg acagagcggg
     1681 cgagagcagt ccccggtcgg aaagggcttc gccggctgtg agtcccacgt agtagatcga
     1741 gaacaccgcc aggccgccgc cgatcacgag ccccatgccg ccgcggggga accgcaacgc
     1801 catcgccagt ccgatcagaa cgaacgaaac gcaggccata ctgatcgccc acttcttgtg
     1861 gacctcgacc atgtaccggt ccgccctcag atccgcatcc ttggcccgat cgagcgcgag
     1921 cgacacttca gcacgggacg tgagccgcag gggtggcgct ggcttcggcc ccgaatcggt
     1981 ggccgacttc acgagcgccg aggcaggcag tttcttcatc gccggtttgg actggggaac
     2041 agctcgagtc ggggccagtt tgcgcgccgg tacatccgga tgtgcggtgc gctgtggcgc
     2101 ggcgggtgtt gccggcggcg ccttgggagc gacggttacc ggcgtgtccg ccggcgtcgt
     2161 tagcgatttg ggctgtttga ctgccgacag aggctgcggc ggagaagggg cgctggtcgt
     2221 ccccacgacg tttgctgtcg aatgcgaatc gggcgcctgg gcggcgagct tcggaggggc
     2281 gagccttgcc cggacccctg tcctgaggcg gtcgagccac aggcaatagt cgcctggagg
     2341 ctctggcgac gtcgtgtccg gctgcagagt aggcagtgca agcagctcgt gcagatcgcg
     2401 ctcgacgaag aaacggcggt ctgcaaacgc tttgtcctgg tccctgtcag catcccgtac
     2461 caccgccagc atcgcgcagc tcgacaactc ccggtcccca cggatctgat ccgaggtatt
     2521 gcgctccaac tcgtcgaaca cgttacgcac gcgaatttcg ttggtcgcga agcgggtgac
     2581 ctgaaagcgg tcggcctcgt ccgacttgta ttgatggacg accccg
//
LOCUS       DASRJQ010000191         2417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1842771, whole genome shotgun sequence.
ACCESSION   DASRJQ010000191 DASRJQ010000000
VERSION     DASRJQ010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1842771"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="VFB89_04385"
     CDS             <1..1088
                     /locus_tag="VFB89_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26568.1"
                     /translation="PGFDAAARAVADAGLPADAPMRAVWCLVAAMVAAKGAVPWARTL
                     LRLTGHFRCGECQSVLPIAALLKKWRTWPLEDSGPPQHSDPAVIADKAGFKPAPGALV
                     PGDFPLRPVAVPDGLHPPVATEVLVVMPDQGGPKKLPWLPGARLKVPWLASVRDGRTL
                     LAVGDATGAVRLCDPSTRTPYGTLFKRPGRPVVGMIFAQADVFRNLVVVYGDLTVDVW
                     SPDAVHGERSDMAPAPDRLQANGHSRIVAVCPGAGLGFRTPILLADRDGTVSMWEPFG
                     VRLSDPLPPDPAHYDVVAVAASAGLVVTAGRASRNLRIWQPLSGKVSLVPLAFAPEWL
                     TFTETTLTAGNADEVISFSVGAEVGPR"
     gene            complement(1555..>2417)
                     /locus_tag="VFB89_04390"
     CDS             complement(1555..>2417)
                     /locus_tag="VFB89_04390"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZM26569.1"
                     /translation="GGHNVISTVFLECGAMFKGDGPEAYRCVGETEFVNGIAAMSASG
                     SYGKTRIAAGIVGTVDLRIGDPAGDVLDAHIAAGGGRFRGIRRGAFWHKSPDIQNHRT
                     NPPEGLLLRDDFRAGFKHLNPRKLSFEVWCAHTQIPDAISLARAFPDTTIVLDHFGGP
                     IGSGPYASKQDEVFGFWKTQIDELARCPNVVAKLGGLNMEVNGYGWEHRSLPPTSTEL
                     MQATRRYFEHTIERFGANRCMFESNFPVDKLSCSYATLWNSFKRLSKGYSADERAALF
                     YGTAARVYRL"
BASE COUNT          398 a          797 c          779 g          443 t
ORIGIN      
        1 tacccgggtt cgacgctgct gcccgcgccg tggccgatgc cggcctgccc gcggatgcac
       61 cgatgcgcgc tgtctggtgc ctggtcgctg cgatggtggc ggcgaagggc gccgtgccgt
      121 gggcgcggac cctgctccgc ctgaccggcc atttccgttg cggcgaatgc caatccgtgc
      181 tgcccatcgc cgcgctgctc aaaaaatggc gcacgtggcc tttggaggat tctggtccgc
      241 cgcagcacag cgaccccgca gtcatcgccg acaaagccgg gttcaagccc gcgcctggtg
      301 ccctggttcc gggcgacttc ccgctacgcc ccgtcgccgt gccggacggg ttgcacccac
      361 cggtcgccac cgaggttctc gtcgtcatgc ccgaccaggg cggcccgaag aagcttccct
      421 ggctgcccgg cgcacgcttg aaggttccct ggctggccag cgtccgagac ggccgcaccc
      481 tgctcgcggt cggcgacgcc accggcgcag tccggctgtg tgacccgtca acccggacgc
      541 cttacggtac actattcaaa cgtcccggcc ggcccgtggt gggcatgatc ttcgcccagg
      601 cggacgtctt ccggaatctc gtggtcgtct acggcgacct gacggtggac gtgtggagcc
      661 cggatgcggt tcacggtgag cgttccgaca tggccccggc tccggaccgg ctgcaggcta
      721 acggccacag ccggatcgtg gccgtctgcc ccggcgccgg cctcggcttc cgcaccccga
      781 tccttctcgc agaccgcgac ggcaccgtat ccatgtggga gccgttcggg gtccggctga
      841 gcgacccgct gccgcccgac cccgcgcact acgacgtcgt ggccgtcgcc gcatcggccg
      901 gactcgttgt cacggccggg agggcgagcc gcaatctgcg gatctggcag cctttgtcgg
      961 gcaaggtttc cttggtccct ctggcgttcg cccccgagtg gctgacgttc acagaaacga
     1021 ccctcaccgc cggcaacgcc gacgaggtga tcagcttctc ggttggcgcc gaggtcggac
     1081 cacggtgatg ccggatcgaa acttgctgaa atgtcgggtc cgcttcggca acggacagaa
     1141 tggtctggcc cttggcacct tttgcacatc gcgtagtcga tccatggccg gttcaattca
     1201 acggacgcga tttgattttc tatcattttt tgtatcaacg ccctgaacgg cgccgcaggc
     1261 ggggcaggag gaacgcgagg ccccgtgcca tgggagatgg ggagcgcggc cggaccatcc
     1321 cattaagcgc aggagatcac cagtatggct gtaattgaag gagcaagaga cgaacgatag
     1381 gcgctcgatg acccattggg tacgggctga aagtgacccg tccgtccgcg actgcacggc
     1441 ggcacgcgaa aagatcatcc gaaacttccg tagcggtgcg gccacaagag attgtggatc
     1501 gaagcggcgt gcaaaaaccg gaacccgtcc cacttttcgc atctcttgag ctgttcacaa
     1561 gcggtaaact cgcgcggcag tgccatagaa cagcgctgcg cgctcatcgg ccgagtagcc
     1621 tttgctgagg cgcttgaacg agttccacag cgtcgcatag ctgcagctca gcttgtcgac
     1681 cgggaagttc gattcgaaca tgcagcgatt tgcgccgaag cgctcgatcg tgtgctcgaa
     1741 gtatcggcgc gtcgcttgca tcagctctgt gctggtcggg ggcaaggatc ggtgctccca
     1801 gccgtaaccg ttgacctcca tgttgaggcc gccaagtttc gcgaccacat tcgggcagcg
     1861 tgccagctcg tcgatctgcg tcttccagaa gccgaacacc tcatcctgct tgctcgcgta
     1921 gggaccgctg ccgatcggac cgccgaaatg gtcgagcacg atcgtcgtat ccgggaacgc
     1981 tctcgcgaga ctgatggcgt ctgggatctg ggtgtgtgcg caccagacct cgaagctgag
     2041 cttgcggggg ttgagatgct tgaagcccgc gcgaaaatcg tcacggagaa gtagaccctc
     2101 gggcgggttg gtgcggtggt tctggatgtc gggactcttg tgccagaagg cgcctcgccg
     2161 gattccgcga aatcggccgc ctccggcggc gatgtgggcg tccaagacat cccccgctgg
     2221 gtcaccgatc cgcaagtcga ccgtgccgac gatcccggcg gcgatccggg tcttgccgta
     2281 gcttcccgac gcactcatcg cggcaatgcc gttcacgaac tcggtctcgc cgacgcagcg
     2341 ataggcttcg ggcccatccc ccttgaacat cgccccgcat tcgaggaaga cagtcgagat
     2401 gacgttgtgc ccgccgc
//
LOCUS       DASRJQ010000192         3031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_4225533, whole genome shotgun sequence.
ACCESSION   DASRJQ010000192 DASRJQ010000000
VERSION     DASRJQ010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4225533"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VFB89_04395"
     CDS             <1..492
                     /locus_tag="VFB89_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26570.1"
                     /translation="SRFARRGEPLTMADLSHSLDRLETRMDRRLRTRLAYDLYARSLG
                     TVSGSLLGFLDHLRLEQAAAAEASRPDIQLMERQTRLLLDQVRSAVENLGPDERKVVE
                     LHFYRSLAAPQIASTMGLPGARRVYTLLDRALVALRGAVHSPPRRAPRPAAKKVRQKR
                     RKS"
     gene            489..1373
                     /locus_tag="VFB89_04400"
     CDS             489..1373
                     /locus_tag="VFB89_04400"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM26571.1"
                     /translation="MNESEQSSSEHIAVEELASLSEPGSAADPAIVAHLARCPRCVAI
                     YAELARTRAHFLHDPEAEMPPADLIQRGMDIPVPAVPSRAVISGRSRQWWLPAAAVLA
                     TVALIWLWPTSSHQPPAVPPDLQTRLSERMRSDSYGGLLYEDSLLPLPSQMRGLGGDS
                     SEADLSVLLRSHRSAETPDAEVDYWLIAGLLATNRQRDADSYIRQSLDRFPDDARFLN
                     LAAILAYKRSDLQLAEKELRAALEHDRSATLLLNLALVLTEAGRSAEAAALLSEFIEG
                     HPSSPAILLARERASTLH"
     gene            complement(1704..>3031)
                     /locus_tag="VFB89_04405"
     CDS             complement(1704..>3031)
                     /locus_tag="VFB89_04405"
                     /inference="COORDINATES: protein motif:HMM:NF017000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lanthionine synthetase C family protein"
                     /protein_id="HZM26572.1"
                     /translation="RATPASNGTRRIERVRADEWQPILEGETSTRAEARVGILLEAIG
                     GWPLPPDLSLGDGHAGLAVVYAYLSRARGLGASSACGHLERAVEGLASQVMGPGFFGG
                     FSGIAWAHEHVVAQLGMNDSEDAADDLDGHLLQLLELPKWERDYDLVEGLVGLGVYAL
                     ERLPHSRAEDMLDRIVDHLDRTSESTPEGLTWLTPPRLLPMSERERHPNGYYNLGVAH
                     GVPGVVALLAAIVAHTRSAPLAGRLLDGAVDWLWAHRLPEGRPGRFATLLEGKEGEAA
                     RLAWCYGDAGIAAAFAAAASALGDVRLRRMAVSLGLVAAARDPGSSGVVDAGLCHGAA
                     GVGHVFNRLFQSTQEEAFLAAARAWFGRAIDLQQEGLGIAGYPSWRVGPDGRVGWQPD
                     PGLLTGTAGIAAALLAATSAAVPEWDRFLLVSLRPAGDCKLRAPAVAGE"
BASE COUNT          521 a         1043 c          978 g          489 t
ORIGIN      
        1 agccgattcg cgcggcgcgg tgagccgctg acgatggcgg atctgtcgca ctcgctcgac
       61 cgactcgaga cgcggatgga ccgccggctg cgcacacgcc tggcttacga cctgtacgcg
      121 cgttctctgg gaaccgtgtc cgggagcctc ctcggctttc tggatcacct gcgactcgag
      181 caggcggcgg cagcggaggc atcgcgtccg gatatacagc tcatggagcg gcaaacgcgt
      241 ctcctcctgg accaggtacg gagtgcggtc gagaacctcg ggccggacga acgcaaggtc
      301 gtcgagctgc atttctaccg gagtctcgcc gcaccgcaga ttgccagcac gatgggtttg
      361 ccgggtgccc ggcgtgtcta cacactcctc gaccgcgcgc tggtcgcact gcgcggtgcc
      421 gtgcactcgc cgccacggcg ggcgccgcgg ccggccgcca agaaggttcg ccagaaaaga
      481 aggaagagtt gaacgaatcg gaacagagct cgtccgaaca catcgcggtc gaggaactcg
      541 cctccctctc cgagcccgga agcgcggcgg atccggcgat cgtcgcgcat ctggctcgct
      601 gtccgcgatg cgtggcgatc tacgccgagc tcgctcgcac ccgagctcat ttccttcacg
      661 atccggaagc cgagatgccc ccggccgact tgatccaacg tggcatggac atcccagtac
      721 cggccgttcc gagccgtgcc gtcatcagtg gccgcagccg gcagtggtgg ctccctgccg
      781 ccgcggttct cgccaccgtt gcgctgatct ggctctggcc cacgtcgtcg caccaaccgc
      841 cggcggtccc accggatctg cagacgcgtc tttcggagcg catgcgcagc gattcgtacg
      901 gcggactcct gtatgaggac tcgctcttgc ctctgcccag ccagatgcga gggcttggcg
      961 gagattcgag cgaggcggac ctctccgtcc tcttgcgctc acatcgtagt gccgagacgc
     1021 ctgacgccga ggtggattac tggttgatcg ccgggctttt ggcgacgaat cggcagcgcg
     1081 acgctgattc ctacattcga cagtcgttgg atcgtttccc ggatgacgct cgattcctca
     1141 acttggcggc gatcctggca tacaagcgca gcgacctaca gctggccgaa aaggagctgc
     1201 gcgcggcgct cgagcacgat cgcagtgcga ccttgctcct caaccttgcc ctggtcctga
     1261 ccgaggcggg tcgttccgcg gaggcggcag ctctcctctc ggagttcatc gagggtcacc
     1321 cctcgagccc cgccattctc ctggctcggg agcgtgcgtc cacacttcac tgatgcgact
     1381 cgaatcggcg cgcgaatcga agtgtctgta tatcgtaatc agggtgtgtg aagcagacct
     1441 gcattacgac ctgcaaggtc gaggagaacg aaccagagac acccgggtcg atgtttgact
     1501 tgaggagagg atgacggcgc gttttcgacg aaagggtcgg gacatgcaac atactcgtgt
     1561 caagaaactc gaattgcacc gcgacacgct tcgctagctc accgatgaga ccatgaagga
     1621 ggtccatggc gcatcggaag acccggtcaa gtggcccccc cacgtggccc gggcagacat
     1681 accccgccac tctttgccga ccatcattct cctgctacgg cgggtgcgcg tagcttgcag
     1741 tctccggcgg ggcgcaacga caccagcagg aagcgatccc actccggcac ggccgcgctg
     1801 gttgccgcca acagcgcggc cgcgatgccc gcggtgcccg tgagcagacc tggatctggc
     1861 tgccagccca cccgaccatc cggcccgact cgccaggaag gatagcccgc aatccccaat
     1921 ccttcctgct gcagatcgat ggcgcggccg aaccaggcgc gcgcggctgc cagaaacgct
     1981 tcctcctggg tcgattggaa caagcgattg aagacgtgtc ccacgccggc ggcaccgtgg
     2041 cacagcccag catcgacaac tccggaggag cccgggtcgc gggcggcggc cacgagcccg
     2101 agactcactg ccattcgccg cagacggaca tcgccgaggg cgctggccgc ggcggcgaaa
     2161 gcggccgcga tgccggcgtc gccatagcac caggcgaggc gagcggcctc gccttcttta
     2221 ccttcgagaa gtgttgcgaa gcgcccgggt cgcccctcgg gcaggcggtg cgcccacaac
     2281 cagtcgaccg ccccgtcgag cagtcgcccc gcgagaggcg cgctgcgggt gtgcgccacg
     2341 atcgcggcaa gcagcgccac cacgccgggc actccatggg ccactccgag attgtaatat
     2401 ccgttgggat ggcgctcccg ctcggacatc ggcagaagcc gcggaggcgt gagccacgtg
     2461 aggccttcgg gagtcgattc ggatgtgcgg tcgagatggt cgacgatccg atccagcatg
     2521 tcctcggccc ggctgtgcgg gaggcgctcg agcgcataca cgcccagccc gacgagtcct
     2581 tccacgaggt cgtaatcgcg ttcccactta ggcagttcga gcagctggag cagatggccg
     2641 tcgaggtcgt ccgccgcatc ctcggaatcg ttcatcccga gctgggcaac gacgtgctcg
     2701 tgcgcccagg cgatcccact gaagccaccg aagaaccccg ggcccatcac ctgggaggcc
     2761 agtccttcga cggcacgctc caagtggccg cacgcgctgg acgcgccgag cccccgggcg
     2821 cggctcaggt aggcgtagac cacggccaac ccggcgtggc catcgccgag cgacaggtcc
     2881 ggggggaggg gccaccctcc gatagcctca aggaggatcc cgactcgggc ctcggcccgg
     2941 gtgctcgtct ccccctcgag gatcggctgc cactcatcgg ctcgcactcg ctcaatccgc
     3001 cgggtaccgt tcgatgcagg ggtagcccgt c
//
LOCUS       DASRJQ010000193         2960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_150744, whole genome shotgun sequence.
ACCESSION   DASRJQ010000193 DASRJQ010000000
VERSION     DASRJQ010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150744"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1275)
                     /gene="mnmG"
                     /locus_tag="VFB89_04410"
     CDS             complement(<1..1275)
                     /gene="mnmG"
                     /locus_tag="VFB89_04410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HZM26573.1"
                     /translation="MQHFDVVIIGGGHAGTEAAMAAARMGARTLLLTQNLETIGQMSC
                     NPAIGGIAKGTVVREVDALGGVMGRATDRARIQFRMLNRSKGPAVWSPRAQCDRGLYR
                     RAVRSILERQPRLLAVQGTVVGLGLSADRVTSVTTADGRRFGAPAVVLTAGTFLRGQI
                     HMGLSTRIAAGRAGDAPAVEIAQAIAALGLSIHRFKTGTPPRVDGRTVVFDGLTRQDG
                     DAEPYWFSSFEQPQHPPQVPCYLTWTNERLREVVTARLKESALYGGEISGRGPRYCPS
                     IEDKIVKFPDAPRHQIFLEPEGLDTSEIYVNGLSTSLPSDVQLTMLRTIPGLERAEMT
                     RPGYAIEYDYFPPTQLRPTLEVRQVEGLFLAGQVNGTTGYEEAAGQGVVAGINAAARA
                     LERPPVVFRRDEAFIGLLVDDLVTRGVDEPYRL"
     gene            complement(1330..1614)
                     /locus_tag="VFB89_04415"
     CDS             complement(1330..1614)
                     /locus_tag="VFB89_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26574.1"
                     /translation="MTGHVFHPGHHALHGVTVLLETTGGLIYIGRFDSEDEAGAHLLD
                     VAMHDSHGSDLPKEDFIRRTLKFGVRVDQKRAVIPRTEIARIGPLSEVSA"
     gene            complement(1616..2044)
                     /locus_tag="VFB89_04420"
     CDS             complement(1616..2044)
                     /locus_tag="VFB89_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HZM26575.1"
                     /translation="MPYDPRLVAPMRDEMVRMGARELKTVAEVDEVLGDQRGTTLVFV
                     NSVCGCAAGNARPALAVALRHAARPQQVVTVFAGQDVEATARARQYFSEYQPSSPSIA
                     LFRDGEVVHFVHRHQIEGQSPQTVAANLTGAFDRFCGATV"
     gene            complement(2101..2556)
                     /locus_tag="VFB89_04425"
     CDS             complement(2101..2556)
                     /locus_tag="VFB89_04425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HZM26576.1"
                     /translation="MTELSGLYQDVILEHNRSPRNFRRMDDADSHAEGRNPLCGDQLT
                     VWLKLSGDRVSDVSFEGSGCAISKASASLMTTAVKGKSRGEIDRLFRQFHEVVTGKSP
                     PQDGSRRELGKLAVFSGIASFPVRVKCATLSWHALKAALERPSGSVSTE"
     gene            complement(2553..>2960)
                     /locus_tag="VFB89_04430"
     CDS             complement(2553..>2960)
                     /locus_tag="VFB89_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZM26577.1"
                     /translation="FEAGTPHIAGVVGLGAAINWITSLGLEAVAAYEHDLLNYATAEV
                     SKIPGVRIVGAARERASILSFAVNGVHPHDVGQVLDAEGIAIRAGHHCAQPVMARFGV
                     AATARASFAAYNTHDEVDALVRGIHRAKQVFGA"
BASE COUNT          564 a          987 c          872 g          537 t
ORIGIN      
        1 aagccggtac ggttcgtcga caccccgagt cacaaggtca tccaccagca gcccgataaa
       61 agcctcatcc cgacggaaga caacgggagg gcgctccagt gcccgggccg ccgcattgat
      121 cccagcaacg accccctggc cagccgcctc ctcatagccg gtcgttccat ttacctgacc
      181 cgccaggaac aagccctcga cctgccgcac ctccagagtc ggccggagtt gcgtcggtgg
      241 gaaatagtcg tactcgatcg catacccagg ccgtgtcatt tccgcccgct cgagtccggg
      301 aatggtccga agcatggtga gctgaacgtc ggaaggcagc gaggtcgata ggccgttgac
      361 gtagatctct gaagtatcca ggccctccgg ctcgagaaaa atctgatgcc gcggagcgtc
      421 gggaaacttc acgatcttgt cttcgatgga cgggcaatag cgtggtccgc gaccgctgat
      481 ctcgcctcca tacagggcag actctttgag ccgggcagtc acgacctcgc gaaggcgctc
      541 gttcgtccag gtgagatagc aaggcacttg cggcggatgt tggggctgct cgaagctcga
      601 aaaccagtaa ggctcggcgt ctccatcctg tcgggtcagg ccgtcgaata ccaccgtgcg
      661 accatcgact cttggcggcg tccctgtctt gaagcggtga atgctaagtc caagcgctgc
      721 aattgcttgg gcgatttcga cagccggtgc atccccggct cgccctgccg caatccgggt
      781 cgacagcccc atgtggatct gtccccgcag aaatgtccct gccgtcaaga cgacggccgg
      841 cgcgccgaat cgcctcccgt cggcagtggt cacactggta actcgatcgg ccgagaggcc
      901 gaggcctacc acagtgccct gcaccgccag aagccgtggc tgtcgctcga gaatcgaccg
      961 cactgcccgc cgatatagcc cgcgatcgca ttgcgcgcgc ggcgaccaca ccgctggtcc
     1021 cttggatcgg ttgagcatcc ggaactggat tcgcgctcgg tctgtggctc ggcccatcac
     1081 cccgcccaag gcgtccacct cgcgcaccac ggttcccttc gcaatccctc cgattgccgg
     1141 gttgcacgac atctgcccga tggtttcgag gttttgggtc agcagcagcg tgcgcgcacc
     1201 catccgggcc gcagccatcg cggcctcggt cccggcgtgg ccgccgccga ttataacgac
     1261 atcgaaatgc tgcataagaa ggaaaggtac gcagggcgcg gcgaatggcc acggaaagac
     1321 acccgatcgc tacgcgctca cctcactcaa cggcccgatg cgcgcgatct cggtccgcgg
     1381 gattaccgcg cgcttttggt ccacgcgcac accgaacttc aaggtgcgac gaataaagtc
     1441 ttccttggga aggtcggacc cgtgcgaatc gtgcatcgcg acatccagga ggtgggcgcc
     1501 ggcctcatcc tcagaatcga agcgtccgat gtagatgagg cctccggtcg tctcgagcag
     1561 cacagtcaca ccatgcagcg catggtgacc gggatgaaaa acgtggccgg tcatttcaga
     1621 ctgtcgcgcc gcagaaccgg tcgaacgctc cggtcagatt tgcagccacg gtctggggcg
     1681 attgcccttc gatctgatgc cgatgcacga agtgaacgac ctcgccgtcc cggaacagag
     1741 cgatcgaggg agagcttggc tggtattcac tgaagtactg gcgggctcgc gcggtcgcct
     1801 cgacgtcctg gcctgcaaac acggtgacga cttgctgtgg ccgcgcggcg tgcctgagcg
     1861 ccaccgcaag cgccggacgt gcgttgccag cagcgcaacc gcacaccgag ttgacgaata
     1921 ccagcgtcgt gcctcgctgg tcacccagca cttcgtcaac ctcggcaacc gtctttaact
     1981 ccctggcgcc catccgcacc atctcgtcgc gcatcggggc caccagccgg ggatcgtagg
     2041 gcatcgctat caagctcctt atggcaaatc cagcagagaa atattcgtac tacacggtcg
     2101 ctactctgtc gatacgctcc cgcttggccg ctccagcgcc gccttgagtg cgtgccacga
     2161 gagcgttgca catttgactc ggaccggaaa gctggcaatc cctgaaaaca ccgcgagctt
     2221 acccagttct cgccggctcc catcctgcgg cggagacttc ccggttacca cctcgtgaaa
     2281 ctggcgaaac aggcgatcga tctcaccccg ggactttccc ttcacggccg tggtcatcag
     2341 cgacgccgat gcctttgaga tcgcgcaccc cgatccctcg aagctcacgt cgctgactcg
     2401 atctccactg agcttgagcc aaacggtgag ctggtcacca cagaggggat tgcgcccctc
     2461 ggcgtgggaa tcggcgtcat ccatccgccg aaagtttcgc ggcgaccggt tgtgctcgag
     2521 gataacatcc tggtagaggc cgctcagctc tgtcatgctc caaacacctg cttcgctcga
     2581 tgaatccctc gaacgagggc atccacttcg tcatgggtgt tatatgcggc gaacgaggcc
     2641 cgggccgtgg ctgcgacgcc gaagcgggcc atgaccggtt gcgcacaatg gtggccggcc
     2701 cgaatcgcga ttccctcggc gtcgagtacc tgaccgacgt catgcgggtg aacgccattc
     2761 acggcaaagc tcaaaatgct agctcgctcc cgggcggcgc ctactatgcg cacaccaggg
     2821 atctttgata cttccgcggt ggcgtagttc aacaggtcat gttcgtacgc ggcgaccgct
     2881 tcgagcccca ggctcgtgat ccagttgatc gctgcgccca gcccaacgac gccggcaata
     2941 tgcggcgtgc cggcttcgaa
//
LOCUS       DASRJQ010000194         2683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1278362, whole genome shotgun sequence.
ACCESSION   DASRJQ010000194 DASRJQ010000000
VERSION     DASRJQ010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278362"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..402
                     /gene="rnpB"
                     /locus_tag="VFB89_04435"
     ncRNA           12..402
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFB89_04435"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            462..1229
                     /locus_tag="VFB89_04440"
     CDS             462..1229
                     /locus_tag="VFB89_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26578.1"
                     /translation="MTSRLFALTALAGVSASAAPSSTHYRVDQTVDQTIDATAIGGAP
                     QRQRFGVSTFITVTLADTAGGKVVNAVIDSVVSDSAPVSQAALDSVRGLPLHGMLDQA
                     GRISGVKPLRETSLGAQMTDVVSRLYPGLRQKVKQGDGWTDTTQVSTPANGGTMNLRR
                     ITTYKASGKERRGNTDALKIDASFSSTVNGTQQTTNGPATIEGTGSGTGAHYVASDGR
                     YLGGKWTVTTKLTFAGSFAPKPLPVTLIETYAVSELP"
     gene            1226..2086
                     /locus_tag="VFB89_04445"
     CDS             1226..2086
                     /locus_tag="VFB89_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26579.1"
                     /translation="MTRSIVVLGLLAAAACSGGTSPSSPAPQPGPRPSGGEKAPTPAA
                     GARGGAILYRPTPGTIYAIERRDSLTLELQGGNQAQQFARTAYLTVSIAEGSGGNYAT
                     TIRLDSLRQEEGGTLSADSLLRAEGTTWTGTLSPQGRLGDLKADRASGVADQVGANLG
                     VLFPVLPAGGLQGATAWSDTAERAIKTDAFAAKERAVTSYRLIKQEGQVYIVDAATGF
                     QRTGTGGQPAQPMEMTSQGTRRTTYQFGADGLVASAQGADSAEMTISVPAVGQTVPVH
                     QRASWKISKK"
     gene            complement(2100..>2683)
                     /gene="dnaN"
                     /locus_tag="VFB89_04450"
     CDS             complement(2100..>2683)
                     /gene="dnaN"
                     /locus_tag="VFB89_04450"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HZM26580.1"
                     /translation="ARMDVPTAAGAGQADLIVPPKALEQIRRLFRPEDEIEIARSENH
                     LGFRSATTQIFTRLIEGPYPNYEQVIPRENDKAATADRSALTAALRRMSIVASDQTHR
                     IRMAFASGSCKLSVQTPDLGEAQEEVTVTYDGDPLEIGFNAAYLLEILKYIPTDEVRL
                     TFKAPERAATCEPVGWNDPASFLTLVMPLRLVD"
BASE COUNT          515 a          834 c          891 g          443 t
ORIGIN      
        1 tcctttaggc gcgggtcgag cggggtggtc gcgggaccgt ttcggttccg aggaaagtcc
       61 gagctccaca gggtagactg ccaggtaaca cctgggcgcc gcgaggcgac ggacagggcc
      121 acagaaaaga caccgccctc gcgcaagcgg gggtaagggt gaaaaggtgg ggtaagagcc
      181 caccgcacca ccggcaacgg atggtggcac ggtaaccccc agtcggagca aggccaaata
      241 agcggcgagg tgtgacccac accaaaaggc cccaagcgca agcaagggcg agccgcgggt
      301 aggctgctcg aggcggtcgg cgacgaccgt cccagacaga tgaccatcct ctcgcgtgag
      361 agcgcgaggg aacagaactc ggcttacggc tcgacccgct ctgccctccg ggctcatctc
      421 ggggggcagt gctttccacc attgcatgct tcgaggatcg gatgacgtct cggctcttcg
      481 cgctcacggc gctggcggga gtctcggctt cggcagctcc tagtagcacg cattatcggg
      541 tcgaccagac cgtcgaccag acgatcgatg cgacggccat cggcggtgcc ccacaacgac
      601 agcggttcgg cgtgtcgacc tttattaccg tcacgctcgc cgacacggcc gggggtaagg
      661 tggtgaacgc agtcatcgat tcagtggtga gcgactcggc tcctgtgtcg caggcagcgc
      721 tggacagcgt ccgcggcctt ccgctgcacg gcatgctgga tcaggctggt cgaatatcgg
      781 gagtaaaacc acttcgcgag acctcactgg gcgctcaaat gacggatgtc gtctcccggc
      841 tttatccggg cctccggcag aaggtcaagc agggtgacgg atggacggac accactcagg
      901 tctcgacacc ggcgaatggc gggactatga atctgcgtcg catcaccact tataaggcta
      961 gcggcaagga acggcgcggg aacacggacg cactgaagat cgatgcgagc ttcagctcga
     1021 cggtgaacgg gacgcaacag accaccaatg gtcccgcgac aatcgagggc accggatccg
     1081 gaaccggggc tcactacgtg gcttcagatg gtcgctatct gggcggcaag tggacggtga
     1141 caacgaagct gaccttcgcc ggctcgtttg ctcccaagcc gctccccgtc acattgatcg
     1201 agacatacgc cgtgagcgag ctgccatgac gcggtccatt gtggtgctcg gcctgctggc
     1261 cgcggcagcc tgttccggtg gtacgagtcc gtcgtctccg gccccccagc cagggccgcg
     1321 tccttccggc ggcgagaagg ctcccacacc tgcagcgggt gcccgcgggg gcgcaatcct
     1381 ttaccggcct acccccggaa cgatttacgc aatcgagcgc cgcgacagtc tcacactcga
     1441 gctgcaaggt ggcaatcagg ctcagcagtt tgctcgaacc gcctacttga ccgtctcgat
     1501 cgccgaggga tccggcggca actatgccac aaccatccgg cttgattcgc tacggcagga
     1561 agagggtggg acgctttccg ccgactccct gctcagggcg gaagggacca cgtggaccgg
     1621 aacgttgtct ccccaaggcc ggctcggcga cctcaaggcg gatcgcgcgt cgggagttgc
     1681 cgaccaggtt ggcgcgaacc tgggcgtact ctttccggtg ctgccggcgg gcggtcttca
     1741 gggagccacc gcctggagcg acacggccga gcgggcgatc aagacggatg ccttcgctgc
     1801 caaagaacgg gccgtgacga gctaccgcct gatcaagcag gaaggccaag tttacatcgt
     1861 cgacgccgcc actggcttcc agcgtaccgg caccggagga cagccggcac aaccgatgga
     1921 gatgacctcg caggggacca ggagaacaac ctatcagttc ggcgccgacg gtctggtcgc
     1981 cagcgcccag ggggccgatt cagcggaaat gactatttcc gtgccggcgg tcggccagac
     2041 ggtgccggta catcagcgag ccagctggaa gatcagcaag aagtagccgc gatgggcagt
     2101 cagtcgacca ggcgcaacgg catcaccagg gtgaggaagc tcgcgggatc gttccaaccg
     2161 accggctcgc aggtggcggc tctctcagga gccttgaagg tgagccgtac ttcgtcggtc
     2221 gggatgtact tgagaatctc cagcagatac gcggcgttga agccgatctc caaggggtcg
     2281 ccgtcgtaag tgacggtgac ctcttcctgt gcctcgccca gatcaggcgt ctgaaccgag
     2341 agcttgcacg acccgctggc gaacgccatg cggatccggt gggtctgatc gctggcgacg
     2401 atgctcatgc ggcggagtgc cgccgtcagg gccgaccgat cggccgttgc tgccttgtcg
     2461 ttttcccgcg gaatgacctg ctcgtagttc gggtacggac cttcgatgag ccgcgtaaag
     2521 atctgagtcg tcgccgaacg gaagccgaga tggttctcgc tccgggcgat ctcgatttcg
     2581 tcctctggac ggaacaggcg acggatttgc tccagagctt tgggcggaac gatgaggtcg
     2641 gcctggcctg caccggccgc ggtgggaaca tccatcctgg cca
//
LOCUS       DASRJQ010000195         8496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3576886, whole genome shotgun sequence.
ACCESSION   DASRJQ010000195 DASRJQ010000000
VERSION     DASRJQ010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 8496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8496
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3576886"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VFB89_04455"
     CDS             complement(<1..237)
                     /locus_tag="VFB89_04455"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZM26581.1"
                     /translation="MKVAIVGGGLTGLAAAYRLQTLGFEPTVFEAAARPGGVVWSERE
                     RGFLAESGPNSLSAPNPIVARLLNELGLADRLIEA"
     gene            complement(241..1542)
                     /gene="hemL"
                     /locus_tag="VFB89_04460"
     CDS             complement(241..1542)
                     /gene="hemL"
                     /locus_tag="VFB89_04460"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HZM26582.1"
                     /translation="MTRTMVASPEAARSASLFAEAQRLMPGGVSSPVRAFKSVGGTPR
                     FIVRGEGSRIVDADDNRYVDLVLSWGPLILGHAHPGVVASVAAAAALGTTFGAPTELE
                     LRLAERVIDAFPSIEMLRFVSSGTEAVMSAVRLARAATGRRAVLKFDGCYHGHADALL
                     AAAGSGIATLGLPDSPGVPSSTVADTLVAPYNDLSAVEALFAARQDGIAAVLVEPVAG
                     NMGVVLPKPGFLEGLRAITTRTGAILIFDEVMTGWRVHRRGAQGLFGITPDLTCLGKV
                     LGGGLPAAAYGGRRNLMEQIAPSGPVYQAGTLSGNPLAMAAGIATLDILSNHGLWERA
                     DHWASRAADAMARAAAAAGTPLTVQRAGTMLTPFFTDSPVHDYAAAKATDRSAFNRFF
                     HSMLDAGVYLPPSAFEAAFTSAAHGDPEFAVLEEALSHAFK"
     gene            complement(1539..2534)
                     /gene="hemB"
                     /locus_tag="VFB89_04465"
     CDS             complement(1539..2534)
                     /gene="hemB"
                     /locus_tag="VFB89_04465"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HZM26583.1"
                     /translation="MSSFPRIRPRRRRRTPALRRLSRETTLSPKHLIAPAFVIHGSAQ
                     SREIASMPGVFQLSVDERLDHEADRLEALGIPAVILFGLPATKDEIGSENFAPDGIVQ
                     RALRRLRARHPDLLLITDVCCCEYTTHGHCGLIRDGTVDNDATLEILGRVAVSHAEAG
                     ADIVAPSGMMDGMVTAIRTALDSAEFQSCSVLSYAVKYASAFYGPFREAADSAPAFGD
                     RRQYQMDPANVREAVLEAGLDEAEGADFLMVKPALAYLDVIRAVREKTKLPLVAYNVS
                     GEYAMLKAAAQRGWIDERRAVLEALTGMRRAGADAIITYYAAEVAGWLQESESTT"
     gene            2781..4283
                     /locus_tag="VFB89_04470"
     CDS             2781..4283
                     /locus_tag="VFB89_04470"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZM26584.1"
                     /translation="MNRSSSLVVILPLLAAAACAAPQRPALVRPRPLDPATAIVDATL
                     WDGTGRAPVPHAVTLVRGDRILCAGGVGECAIPRDARVIDAHGAWLVPGLIDTHVHLL
                     FLRRGSASEELGSDLKDLLAQGITTVRDMGTNPKELLSRTRAFGAAPRVYAMQLVAGF
                     RFFYGREAVPTSDGSVAYRLPQAQIMEMRGWTPMMFTHDDDPDSVAAAAQAAGAIGLK
                     LYGYLDSTSVRMLTEAAHRAGMTAWGHAWVQPANARDQVLAGEDGVVHASLFVGELLA
                     PQTRDSLLASTAILEEGARSASPEAALDQRIGETLDSMARRGTFLEPTLDVSLRSVSH
                     FDAEHADRPSLPEQYARAVVGFSMAVTREAVKRGVRITAGTDHVAYGPVRDRASLVSE
                     LRLLVDSAGLTPEQALLAATRNAAQAIGGDAARSVGTIEAGRYADLLLLDKSPLEDIR
                     NLDQVQWVMQAGRLWRPWQLRSGMASRTGAKSSAEPADADAEENLGLTHP"
     gene            complement(4287..5114)
                     /locus_tag="VFB89_04475"
     CDS             complement(4287..5114)
                     /locus_tag="VFB89_04475"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZM26585.1"
                     /translation="MADWQRRGEAFRLIDVRTDPFVYLKDHLPEAVYLNTETLRAAAG
                     GTPMQLMPAEWYAQVFARVGIRWDLPVVVYSAGETHNIDATFLVWLLASYGHPRVYLL
                     DGGYFKWQLEQRPLAKHYPKLVASPAPGGAFKPATATLDDVRSAAERRDVVLVDARPP
                     DQFGGEAGAQMRRGHIPGAINHYWQDDLEQEGFGRVWKPADALRAMYAAQGITPDKRL
                     ILYCNSTTEASHVFFALKYLLGYPDVRIYTGAWTEWAERSDLPIESGAAPAQAAGRH"
     gene            complement(5288..6241)
                     /locus_tag="VFB89_04480"
     CDS             complement(5288..6241)
                     /locus_tag="VFB89_04480"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZM26586.1"
                     /translation="MFSLLALHTVMTGWSVSALPSTAPLFPLRDLGHGVYAVLGDTGR
                     GSEGRANAGFVVTRDGIVVIDAEGSPRDGETLLRTIRSVSQRAIRWLVLTHHHPDHHF
                     GAIVFRKAGAKVIAHPDSRTLASEGGLDNQMIDWIRVVGLDAMRGFEYADTPDRPVTK
                     GDTLRIGGRRVIVLAPGPAHTPGDLMVWVPDAGVLFAGDILLEDGVTMVVDGSAPALL
                     KALNQIDSLRPKVVVPGHGSVPAEPAVLVERTRAYITQLRGEMRAAVQEGVPMKRALD
                     ALPPADENRPVSLNSRKRRNAARVYVEEEKDIMGLSGDSGQ"
     gene            complement(6263..6598)
                     /gene="soxZ"
                     /locus_tag="VFB89_04485"
     CDS             complement(6263..6598)
                     /gene="soxZ"
                     /locus_tag="VFB89_04485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HZM26587.1"
                     /translation="MSNVGDARLRIPDRIAKGDIITVNSIVSHPMDTGFFRTKEGAPI
                     PAYFINDVVVTYGGEQIARFEWTSGISRDPVVSFSLKADREGPLVMTWKDNRGGVYSQ
                     TVNIAFAAA"
     gene            complement(6601..7110)
                     /locus_tag="VFB89_04490"
     CDS             complement(6601..7110)
                     /locus_tag="VFB89_04490"
                     /inference="COORDINATES: protein motif:HMM:NF024892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HZM26588.1"
                     /translation="MLDQLGLPRRSFVRRIVLAAVGIAGSSAIIDPKRLLALAAGGGP
                     DDPEIPNEAVAKIYRERFAGRPITRGHVQLDMPAVAEDGRIVPVIIESDLPMTAEKYV
                     KGIHLIVDHNPDAHLAGFYLSPAIGQVSITTRIKMKRTTWVRAIAETNTGELWADYAK
                     VLVTLNGCG"
     gene            7461..>8496
                     /locus_tag="VFB89_04495"
     CDS             7461..>8496
                     /locus_tag="VFB89_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZM26589.1"
                     /translation="MDIQTLQQAALLDKCRQFTRAREVQAAGLYPYFKPISESEDTVV
                     VIEGQKRLMLGSNNYLGLTHHPKVLEAASRALSRYGSGCTGSRFLNGTLDLHEQLEHA
                     LAEFTSKESCLVFSTGYQANLGLISGLVGRGEVVYLDKLDHASIVDGAKMSYGDTERF
                     NHGDLAALERKMDRGLSGRGAMIVVDGVYSMEGDIADVPGLVRVARKFGAVLAVDDAH
                     SVGVLGPNGDGTAAHFGLTEEVDIIAGTFSKSLASIGGFVAAPESVIHYLRHHCRPLM
                     FTASLPPANTAGVLAALEVLQQEPERREQLWANTRRLQDGFRSLGFDIGPTETPIVPV
                     LVGAMEKTFLM"
BASE COUNT         1537 a         2755 c         2658 g         1546 t
ORIGIN      
        1 ggcttcgatc aaccggtccg ccagtcctag ctcgttcagc aacctggcca cgatcggatt
       61 gggcgcgctg agggagttcg gcccgctctc ggcgagaaat ccacgctcgc gctcgctcca
      121 aacgacgccg ccgggccggg ccgcggcctc gaaaaccgtc ggttcgaagc cgagggtctg
      181 gagtcgatac gccgccgcca gcccggttag gccaccgccg acgatcgcga ccttcatacg
      241 ctatttgaag gcgtgggaaa gcgcctcttc gaggactgca aactctgggt ccccgtgagc
      301 agcggatgtg aaagctgcct cgaaagccga gggcggcaga tacactccgg catcgagcat
      361 ggaatggaag aatcgattga aggccgaacg gtcagttgcc ttagcagcgg catagtcgtg
      421 gaccgggctg tcggtgaaga acggcgtgag catggtgccg gcccgctgca cggtaagagg
      481 agtacctgcg gcagctgcgg cacgcgccat tgcatcagct gcccgcgacg cccaatggtc
      541 tgcacgttcc cacagaccgt ggttggacag aatgtcgagc gtcgcgatgc ctgccgccat
      601 cgcgagcgga ttgcccgaca gcgtgccggc ctgatagacc ggtcccgacg gtgctatctg
      661 ctccatcagg tttcggcgcc caccatatgc cgcggccggc aagccgccgc caaggacctt
      721 gccaaggcag gtgagatcag gcgtgatccc gaacagtccc tgcgcgcctc gccggtgcac
      781 tcgccacccg gtcatgacct cgtcgaagat cagaatggcg ccggtacgag ttgtgattgc
      841 gcgaaggccc tcgaggaacc ctggcttggg caggacgacc cccatgtttc ccgcaaccgg
      901 ctcgaccagc accgccgcaa tgccatcctg tcgcgccgca aataaagcct cgacggcgct
      961 cagatcgtta tacggagcga ccagggtgtc ggcgaccgtg ctactcggca cgccgggaga
     1021 gtccggcaag ccgagcgtag cgattccgga gcctgccgca gccaacaatg catcggcgtg
     1081 gccatggtag catccgtcga atttgagcac tgcccggcgt cccgtcgcag cccgagccag
     1141 gcgcaccgca ctcatcactg cctcggtacc cgaactgacg aaacggagca tctctataga
     1201 tggaaacgca tcaatcaccc gctccgccag acgcagttcg agctcggtgg gtgcgccgaa
     1261 cgtggttccg agcgcggcgg cggcggcaac ggacgcaacc actcctggat gagcgtgccc
     1321 taaaatcaaa ggtccccagg agagcaccag atcgacgtag cgattgtcgt cagcgtccac
     1381 tattcgcgag ccttcaccgc gaacgatgaa ccgcggcgtt ccgccgacgc tcttgaatgc
     1441 ccggaccgga ctgctgacac cgcccggcat caatcgttga gcttcggcaa agagcgatgc
     1501 tgatcgtgcg gcctcggggg aggccaccat ggtgcgtgtc atgtggtcga ctccgattcc
     1561 tggagccatc cggcgacctc tgccgcgtaa taggtgatga tggcatccgc cccggcccga
     1621 cgcatgcccg tgagcgcctc aaggaccgcg cgccgctcgt cgatccagcc ccgctgagct
     1681 gcggccttta gcattgcgta ctcgccactc acgttgtacg ccaccagtgg aagcttggtc
     1741 ttctcccgca cggcgcgaat cacgtcgagg taggccaaag ccggcttaac catcaggaag
     1801 tcggctccct cagcctcatc cagtccagcc tccagaaccg cttcccgcac gtttgccgga
     1861 tccatctggt actgacgccg gtctccgaac gcgggggcgc tgtcggccgc ctcgcggaat
     1921 ggtccgtaga acgcgctggc atacttgacc gcatagctca gcaccgagca gctctgaaat
     1981 tccgccgagt cgagtgcggt gcgaattgcc gtgaccatgc catccatcat tccgctcggc
     2041 gcgacaatat cggcgcctgc ctcggcatgg ctcactgcca cccggcccag aatctcgagc
     2101 gtggcatcgt tgtccaccgt gccgtcgcgg atcaggccgc agtgaccgtg agtggtatac
     2161 tcgcagcagc atacgtccgt tatgagcagg aggtccggat gccgcgcccg gagtcgccgc
     2221 agtgcccgtt gcacgatgcc atcgggcgcg aagttctcac tcccgatctc gtccttggtc
     2281 gcggggagcc cgaacagaat caccgccgga atgcccaacg cttccagccg gtcggcctcg
     2341 tgatccaatc gctcgtcgac ggagagctgg aacacccctg gcatcgacgc gatctcccga
     2401 ctctgcgcag aaccatggat cacaaacgcc ggcgcaataa ggtgcttagg gctgagcgtg
     2461 gtttcgcgag acagccggcg aagtgccggc gtgcgacgcc ggcgtctggg ccggatcctg
     2521 gggaaagagc tcacgggctt gcgggctcga ggcgtgtgga aacgatcagc tggcagaccg
     2581 gcgaaagcta cgggccctga cagccgcgta caagcaaatg cactaccgag acttgaagca
     2641 atggatcacc ccccggaacg cgtacagtct ttgcatgttg ctcctgacga acccggctga
     2701 ggtcggaatt gttcagcagc atacctgtcg gagtcgcgtc ttgcggcggc cgacggcagt
     2761 cgcccgggca gaggtgtagc atgaatcggt cgtcgagcct cgtcgtaatt ctccccctcc
     2821 ttgcggcagc tgcgtgcgca gctccccagc gccctgcgct ggtgcgtccc cgccctctcg
     2881 accctgcaac tgccattgta gacgcgactt tatgggacgg gaccggccgc gcaccggttc
     2941 cccacgcagt cactctggtc cgcggtgacc gtatcctgtg cgccggcggc gtaggcgaat
     3001 gcgcgatccc ccgcgatgcg cgggtgatcg acgcgcacgg cgcctggctg gtgccgggac
     3061 tgatcgacac ccatgtccac ctgttgtttc tccgccgcgg cagtgcgagc gaagagctgg
     3121 ggagcgatct caaggatctg ctggctcagg gcatcaccac agtacgcgac atgggcacga
     3181 accccaagga gctgttgagc cggacccggg ccttcggtgc tgcaccgcgg gtgtacgcca
     3241 tgcagctggt cgccgggttc cgcttcttct atggtcgcga ggcggtcccc accagcgatg
     3301 gcagtgtggc ttatcgcctg ccgcaggcgc agatcatgga aatgcgtggc tggaccccga
     3361 tgatgttcac tcacgacgac gatccggatt ctgttgcggc ggccgctcag gcagctggtg
     3421 cgatcggcct caagctctac ggctatctcg actcgacgtc ggttcgcatg ctgaccgagg
     3481 ctgcccatcg tgccggaatg acggcatggg gtcatgcgtg ggttcagcct gcgaatgcta
     3541 gggaccaggt cctggccggc gaagatgggg tggtgcacgc gtcattgttc gtgggcgagc
     3601 tgctggctcc gcagactcgg gacagtctgc tcgcgagcac ggcgattctg gaggaaggag
     3661 cccggagtgc ctcgccggag gccgcgctgg atcaacggat cggcgaaacg ctcgacagca
     3721 tggcacgccg tggcacgttc ctcgagccga cgctcgacgt ctcactgcga agcgtctccc
     3781 acttcgacgc tgaacatgcc gaccgaccct cgttgccgga gcagtatgcc cgcgcagtag
     3841 tcgggttcag catggccgtg acgcgcgagg cggtgaaacg gggagtgcgc attaccgctg
     3901 gtaccgacca cgtcgcctac ggtccggtgc gcgaccgcgc gtcattggtc agtgagttgc
     3961 gattgctggt tgactcggcc ggtctcactc cggagcaggc tcttctcgcg gcaacccgca
     4021 atgcggcgca ggccatcggc ggcgacgccg cgcggtctgt cgggacaatc gaagctgggc
     4081 gctacgctga cctcttgttg ctggacaaga gtcccctcga ggacatccgc aatctcgatc
     4141 aggtgcagtg ggtaatgcag gcaggacggt tgtggcgacc ctggcagctg cgaagcggaa
     4201 tggcaagccg aaccggagcg aagtcctcgg cggagcctgc cgacgcagac gccgaagaga
     4261 acctcggcct tacccacccg tagcgcctag tgcctcccgg cggcctgcgc gggcgccgct
     4321 ccgctttcga ttggcaggtc ggagcgctcg gcccattcgg tccaggcacc ggtgtagatc
     4381 cgcacatcgg ggtatcccag caaatacttc agcgcaaaga agacgtggga cgcctcagtg
     4441 gtgctgttgc agtagaggat gaggcgcttg tcgggcgtga tgccctgggc ggcatacatc
     4501 gctctcaatg cgtccgccgg cttccatacc cttccgaatc cctcctgctc aaggtcgtct
     4561 tgccagtagt ggttgatggc gccgggaata tgcccccgcc gcatctgggc cccggcctcg
     4621 cctccgaatt gatccggcgg acgggcatcc accagaacca catcgcgacg ctcggccgcg
     4681 ctgcgtacgt cgtcgagcgt tgccgtcgcc ggcttgaacg caccgcccgg cgcaggggag
     4741 gcgaccagct tggggtagtg tttcgccaag ggacgctgtt ccagctgcca cttgaagtag
     4801 ccaccatcaa gcaggtatac ccggggatgc ccgtagctcg cgagaagcca aaccagaaag
     4861 gtggcgtcga tgttgtgcgt ttcaccggca ctgtaaacca cgactggcag gtcccagcgg
     4921 ataccgaccc tggcaaatac ctgagcgtac cactctgccg gcatcagttg catcggagtg
     4981 ccgcccgctg cagcccgtaa ggtttcggtg ttcaggtaaa cggcctcggg aaggtggtcc
     5041 ttcaggtaaa cgaacggatc cgtgcgcacg tcgataagcc ggaacgcctc gccacgccgc
     5101 tgccaatcgg ccaattgatc cgtagagatc agggagggcc gtatcgaatt ctgtggtgct
     5161 ggagtctgcg aggttgccgg cttccctgca caggagatca gcatcagtgc agcgctaacg
     5221 acgagaccga gccgcaacgc tctcgtcccg agcggaactc cggtgcgacc aacgctgtgg
     5281 tagctgctca ttgccctgag tctccactca atcccatgat gtccttctct tcctccacgt
     5341 agaccctggc cgcattccgc cgcttccgag aattgagcga taccggccga ttttcgtcgg
     5401 caggcggcag cgcatcgagc gcccgcttca tcggcacgcc ctcctgcact gcagcgcgca
     5461 tttcaccgcg aagctgggtg atgtatgctc tggtgcgttc caccagcacg gccggctcgg
     5521 ccggtacgct gccatgtccg gggaccacga ctttgggacg cagcgagtcg atctggttca
     5581 acgctttgag cagcgccggc gcgcttccgt ccacgaccat cgtaacgcca tcctctagca
     5641 ggatgtcgcc cgcgaacagg acccctgcgt cgggcaccca caccatcagg tctcccggcg
     5701 tgtgcgccgg tcccggtgca agcacgatca cgcgccggcc gccaatgcgg agagtatcac
     5761 ccttcgtcac gggacgatcg ggcgtgtcgg catattcgaa tccgcgcatc gcgtcgagtc
     5821 caaccactcg aatccagtcg atcatctgat tgtccagccc accttccgag gccaacgttc
     5881 tgctgtcggg atgcgcaatt accttggcgc ccgctttccg aaagacaatg gctccgaaat
     5941 ggtgatcagg gtggtggtga gtcagcacca accatcgaat agcacgctgc gagacagaac
     6001 ggatcgttcg gagcagggtt tcgccgtcgc gggggctgcc ttccgcgtcg atgacgacga
     6061 taccgtcgcg ggtgaccacg aaaccggcat tggctcgtcc ttccgacccg cggccggtgt
     6121 cacccaggac cgcatacacg ccgtgcccga ggtcacgcag ggggaacagc ggagcggtgg
     6181 acggaagagc agaaacgctc catccggtca tcaccgtatg gagcgcgagc agagagaaca
     6241 cgaaaaaaga ctggccgcga gatcaggctg cggcgaacgc gatgttgacc gtctggctgt
     6301 agactccgcc cctgttgtcc ttccaggtca tcacgagcgg tccttcccga tccgccttga
     6361 ggctgaagct cacgaccgga tcgcgactga tgcccgacgt ccactcgaag cgggcgattt
     6421 gctcgccgcc ataggtgact acaacatcgt tgatgaagta ggcgggaata ggagcgcctt
     6481 ccttggtcct gaagaatccg gtatccatcg ggtgcgacac gatggaattc acagtgatga
     6541 tgtcgccctt ggcgatccgg tctggaattc gaagccgcgc gtcgcccacg ttcgacattg
     6601 tcagccgcat ccgttcagcg tgaccagcac tttggcgtag tcagcccaca actcgcccgt
     6661 gttggtctcc gcgattgccc ggacccaggt cgtacgcttc atcttgatcc gggtggtgat
     6721 ggatacttgt ccgatggcgg gtgacagata gaatcccgcc aaatgtgcgt ctggattgtg
     6781 atcgacgatc aggtgaatgc ctttgacata cttctcggcg gtcatcggca ggtcgctctc
     6841 gatgatgacg ggaacgatgc gaccgtcctc ggccaccgca ggcatgtcga gctggacgtg
     6901 ccccctggta attgggcgcc ccgcgaagcg ctcgcgatag atcttcgcca cagcctcatt
     6961 cggaatttcg ggatcgtcgg ggccgcctcc ggccgccagc gccagcaacc gcttcgggtc
     7021 gatgatcgcg ctcgatccgg caatgccgac ggcagccagc acgatgcgcc gcacaaaaga
     7081 tcgccgtggc aacccgagct gatccaacat gcagcctccg aaaatccgcc gacccgaacc
     7141 caccctgatc actaacatat ttcaatcgca ttggattcgc agcagaatcg gccgctgccg
     7201 ggccccctcc ccgatttgag gcccccgtcg tccggtcccg aacttggtac cgagccatgc
     7261 tttgcgcgcc ctccgtccga gtgtcgcaaa cttgcggggc caagacggcc cgagccttgc
     7321 tatccccttg cccgatttgg cattccagac attgccggtt acgctgagtc agtcttgtgt
     7381 gggaggtcgg agcggcgtac tttaagccgt tctggacaaa tgctttcggg gccatttacc
     7441 ctttgccgag ccgaggcacc gtggatatcc agaccctgca acaggccgcg ctgttggata
     7501 agtgccgtca gtttacgagg gcgcgcgaag ttcaggcggc gggattgtat ccctacttca
     7561 aaccgatttc cgagtccgag gacacggttg tcgtcataga aggtcaaaag cggctcatgt
     7621 tggggtccaa taactatctg ggcctcaccc accaccccaa ggtcttggag gctgcgtcgc
     7681 gggctctttc ccggtacggc tcgggctgca ccggaagccg gttcctgaac ggcaccctcg
     7741 atctccatga gcagctggag cacgccctcg ccgagttcac aagcaaggag tcgtgcctgg
     7801 tcttttcgac cggctaccag gcgaacctcg gcctgatctc agggctcgtt ggtcgtggcg
     7861 aggtggtcta cctcgataag ctggaccacg cgtcgatcgt cgacggcgcc aagatgtcgt
     7921 acggcgacac cgagcgcttc aaccacggcg acctcgctgc tctcgaacgg aagatggacc
     7981 gtggcctgtc gggtcgcggc gccatgatcg tcgtggacgg cgtgtactcg atggaaggcg
     8041 acattgccga cgttccgggt ctggtgcggg tggcccggaa gttcggtgcc gtgctcgccg
     8101 ttgacgacgc acattcggtc ggcgtgcttg gacccaacgg agacggtacc gcggcccact
     8161 tcggtcttac cgaggaagtg gacatcatcg cgggcacgtt ctccaagtcg ctggcgtcga
     8221 tcggtggatt cgtagccgca ccggagagcg tgattcacta tctgcggcac cactgccgcc
     8281 ccctgatgtt caccgcatcg cttcccccgg caaacacggc tggcgtactg gcggcgctcg
     8341 aggtgctgca gcaggagccg gagcgccgtg agcagctctg ggccaatacc cgtcgtctgc
     8401 aggacggttt ccggagtctc ggcttcgata tcggtccgac cgagaccccg attgtgcctg
     8461 tgctggtggg cgcgatggag aagaccttcc tcatgt
//
LOCUS       DASRJQ010000196         3412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_714830, whole genome shotgun sequence.
ACCESSION   DASRJQ010000196 DASRJQ010000000
VERSION     DASRJQ010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714830"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="VFB89_04500"
     CDS             <1..1048
                     /locus_tag="VFB89_04500"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZM26590.1"
                     /translation="VTFAGPSRSDPDGLETLRRLGITLHHVWRSPAHGLAVLLRRARL
                     TTRWASGSDPMRALKFQDPAMQAALDEAARGSRFDIVLVEDSAMGQYRYPAGIPSVLT
                     DNDVRSADEGVEARRWHAYQRTVWRRFDRLQVYTERDAAGLRRLAPDLAERVRVNPFG
                     VDLEPATDSTAEREDELVFVGGFHHSPNVDAACWLVEEIFPLVRAKRGSAHLTIVGAD
                     PPRAVRALASERVTVTGFVPDVAPFLQRAAVVVAPLRRGSGMRVKLLQALARGKAVVT
                     TPLGAEGLAPDAPLRVASSAPDIATAVIALLNDAEARRSLGVGARGFVAEHHSWSAFV
                     DRLAATCRELGLDP"
     gene            1045..2157
                     /locus_tag="VFB89_04505"
     CDS             1045..2157
                     /locus_tag="VFB89_04505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZM26591.1"
                     /translation="MIRTVVQYVDSSIFGGSEQVLLQLLAGLDRGRWRPVLLHHPEPG
                     LARLVDGARSAGVQVDSVPRVTDRNFPIRLPQLVSAIATQRPAVVHAQLNWPLACKFG
                     LLAATLGRVPAVVATVHLVIEELLNGSVRLQGRIFGVGVHRYLAVSEHVRGRLAAGMG
                     WPEQKLRVVPNGIDPAPFSQPFDPALRQWLTRGSQRPLVFTAARLSPEKAIDVLVSAA
                     ARVPEAVFAIAGDGPERAAIRMQVEALGISERVRLLGARSDIPALLAACDLFVLPSLV
                     EGLPISVLEAMAAARPVVATRIGGTDEVVMDGRTGLLVPPQDPEPLAAAIRSFLADPE
                     RGRQAGKAGREQLLARFTSKVMIDAVSATYEELLAR"
     gene            2154..>3412
                     /locus_tag="VFB89_04510"
     CDS             2154..>3412
                     /locus_tag="VFB89_04510"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HZM26592.1"
                     /translation="MTKPVTEPPIREAARDRELRRRDWRFLRPGTNLQHIQPPRTPAL
                     QGFAMRNPPGQGVYAEWRYPVPGGGTAIRKRLESSGFQNVDLYWPWPGLRRPWFWLPL
                     GSEAAAAYIRATRLPARSLVRRVMDRPLRAVWHWAAARQRLWPICAVARVADSSSSTH
                     DLLDRIGREWAAWGLGQRPDRLSWLLLTRGARSINKVVGLVFAEPDPSPRVVVKLPRA
                     PDVHDALRREATVLRAVHRRLSTGLPGAPRVLFCDEQSGGLTLCETALDGRPLFSLLA
                     KSTYRDLAMKATEWLTELAGNPSDGGARAGANDVGAVTQEFATMFGAVVNPMELWTTE
                     RLLAPLADLPTVPEHRDFSPWNVHVAPDGALAVYDWESAEPGGFPLNDLIYFLTYLAF
                     FYDRAIAGGSCVESYRRARDPGTFTGGV"
BASE COUNT          509 a         1157 c         1132 g          614 t
ORIGIN      
        1 cgtcaccttc gcgggcccga gccgctccga tcctgatggg ttggaaaccc tcaggcgcct
       61 cggcatcacc ctccaccacg tgtggcgcag tcccgcccac ggactcgccg tgcttctgcg
      121 gcgggcacgc ctgacaacgc ggtgggcttc gggcagcgat ccgatgcggg ccctcaagtt
      181 ccaggaccct gcgatgcaag cagcgctcga cgaggcagcg cgtggcagtc ggttcgatat
      241 cgtgctggtt gaagacagcg cgatgggaca gtaccgatat ccggcgggca tcccctccgt
      301 gctcaccgat aacgatgtcc gctctgcgga tgagggtgtg gaggcccggc gctggcacgc
      361 ttatcaacgg accgtctggc gtcggttcga ccggctccag gtgtatacgg agcgcgacgc
      421 cgccggcctg cgccgcctcg cgcccgacct cgccgagcgg gttagagtaa acccgtttgg
      481 cgtggatctc gaacctgcaa ccgattcaac ggccgagcgc gaggacgagc tggtgttcgt
      541 gggcgggttc catcacagcc ccaacgtgga tgccgcatgc tggctcgtgg aggagatctt
      601 cccgctcgtt cgcgcgaagc gcgggtcggc tcatctcacg atcgtaggtg ctgacccgcc
      661 cagagccgtc cgcgccttgg cctcggagcg ggtgactgtg accgggttcg ttcccgacgt
      721 cgcgcccttc ctccaacgcg cggccgtcgt ggtggcgcct ctccgaaggg gcagcgggat
      781 gcgggtaaag ctgttgcagg ctctcgcgcg cggcaaagcg gtcgtcacca cgccgctcgg
      841 cgctgaaggg ctggcgcccg acgcgccgct ccgcgtcgcg agttctgccc ctgacattgc
      901 gaccgccgtc atcgcgcttc tcaacgatgc ggaggcgcgc cgctcgctcg gcgtcggcgc
      961 gcgcgggttc gtggcggagc atcactcctg gtccgccttc gtagatcggt tggctgcgac
     1021 ctgccgcgaa ctcgggctcg acccatgatc cgcactgtag tgcagtacgt cgacagcagc
     1081 atcttcggcg gaagtgagca ggtcctcctc caactgctgg cggggctcga tcgcggccgg
     1141 tggcgccccg tgttgctgca tcatcccgaa cccggactcg cccgtctcgt cgacggtgcc
     1201 cgctcggctg gcgtacaggt ggactcagtt ccacgggtaa ccgaccggaa tttcccgata
     1261 cgacttcccc agctcgtaag cgcgattgct acacagcgcc ccgccgttgt tcacgcgcaa
     1321 ctcaactggc ccctggcgtg caagttcgga ctgctcgcgg cgacgcttgg cagagtcccg
     1381 gccgttgtag cgaccgtgca cctcgtcatc gaagaactgc ttaacggcag cgtgcggttg
     1441 caggggcgga tattcggcgt tggagtgcac cggtacctcg ccgtgtcgga acacgttcga
     1501 ggccggctcg ccgccggcat ggggtggccg gagcagaagc tcagggtagt cccgaacggg
     1561 atcgaccctg cgcccttttc gcaacccttc gatcccgccc tccgccagtg gctcactcgc
     1621 ggctctcagc gcccacttgt gttcaccgca gcccgtctca gccccgaaaa agcgatcgac
     1681 gtcctcgtct ccgcggccgc tcgcgtgccg gaggctgtgt tcgcaatcgc gggagacggt
     1741 cccgagcgcg cggctatcag gatgcaggtc gaggcactgg gaatctcgga acgagtaagg
     1801 cttctcgggg cgcgaagcga catacccgcg ctattggccg cttgtgacct atttgtcctg
     1861 ccgtcactgg tggaggggct tcccatctcc gtgttggagg caatggccgc tgccaggccg
     1921 gtggtcgcaa ctcgaatcgg tgggactgac gaggtggtta tggatggtag gaccggtctg
     1981 ctggttcccc cgcaggaccc ggagccgctt gccgccgcca tccgaagctt tctcgccgat
     2041 cccgagcggg gccggcaggc tgggaaggcc gggcgggaac agcttctcgc gcggtttacg
     2101 tcgaaggtga tgatcgatgc cgtcagcgcg acctacgagg agctgctcgc gcgatgacaa
     2161 agccggtcac ggagccaccc attcgtgagg cagctcggga tcgggagctg cgccggcgtg
     2221 attggcggtt tctacgtccg gggacgaacc ttcaacacat ccagcctccc cgcacgccag
     2281 ctctgcaagg tttcgcgatg cgcaatccac ccggacaggg agtctacgcc gagtggcggt
     2341 accctgtgcc cggtggtgga acagcaatcc ggaagcgact cgaatcgtcg ggcttccaga
     2401 acgtggactt gtactggcca tggcccggcc tccgtcggcc ctggttctgg ctgccgctcg
     2461 gcagcgaggc ggcagcggcc tacatccgcg ccacccgctt gcccgcccgg agcctggtcc
     2521 gtcgggtcat ggaccgcccg ctccgcgctg tctggcactg ggccgcagcc cgccagcgac
     2581 tgtggccaat ctgtgcggtg gctcgcgtgg ccgactcttc ctcatccacc cacgacctgc
     2641 tggaccgcat cgggcgtgag tgggcggcgt ggggcttggg ccagcggccg gaccgcttgt
     2701 cgtggctgct tctcacccgc ggggcgcgaa gtatcaacaa agtcgttggg ttggtttttg
     2761 ctgagcccga cccgagtccc cgcgtcgtcg tcaagttgcc gcgcgccccg gatgtccatg
     2821 atgcgcttcg ccgtgaggca acggtcctca gggccgttca tcggcgcctc tcgactggac
     2881 ttcccggagc gccccgcgtg ctcttctgcg acgaacaaag cggcgggctg actctctgtg
     2941 agaccgcgct cgatgggcgg ccgctcttct cattgctcgc caaaagcacc tatcgagacc
     3001 tcgccatgaa ggcgacggaa tggctcaccg agctcgccgg aaacccttcg gacggcggag
     3061 ccagagcggg cgcgaacgac gtcggagctg ttacacagga attcgcgacg atgttcggtg
     3121 cggtggtgaa tccaatggag ttgtggacaa ccgagcggct gctggcgcca ctggcagatc
     3181 tcccgaccgt gcccgagcac cgcgacttct cgccgtggaa cgtgcacgta gcccccgacg
     3241 gggcgctcgc ggtatacgac tgggaatctg cagagcccgg cggcttcccc ctcaacgatc
     3301 tgatctactt cctcacctac ctcgccttct tttatgaccg cgccattgcc ggcgggagct
     3361 gcgtcgagtc gtaccgccgg gcgcgcgacc cgggcacttt caccggcggg gt
//
LOCUS       DASRJQ010000197         2118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_714729, whole genome shotgun sequence.
ACCESSION   DASRJQ010000197 DASRJQ010000000
VERSION     DASRJQ010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714729"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..1707
                     /locus_tag="VFB89_04515"
     CDS             220..1707
                     /locus_tag="VFB89_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26593.1"
                     /translation="MSIFRKLRLLSAGALALATASAFFAQPATAAHRKSAAAAALDTA
                     IARMGGQETLAHIERVRFEMMTLWQRMTFDTRPTDLISTYELHSDLRNYPLVAWRNTR
                     RFVSGPQMQEMTDIVQKDAGVRRFAPKPDGTASPWAPINIAYIDERKEVFAFAPERLL
                     LAAHAASDLRELSDTTIAGQPHTRLSATIDGFPTTIFLRRNDGFLAMARYRAGQPNDF
                     GLAPWGDMETEMWYSRWTKFPVPGTPGVAYPTQVDVRRVGRMYKRLVVLSANFDAAAP
                     ADSFAISDSLRTAFESGPSSKPMWEVPMDSAKLLEPRLARLGNPGQTQAAVKLGKSWL
                     FLEGASVPQRSETDVRWLESADPGSTIAGLLVTAPNTGRGGAAWFTQRKLPVYVTPGA
                     VSAMGTTLANWKQPPSAVKVVSKSQWLRISGDSVWVEPIDLPDYPGALVAYVPSLRWV
                     YSGPAATPLYFDMVVARIRDRGWAVDRIGSVRSLTQPIPARTASR"
     gene            1883..>2118
                     /locus_tag="VFB89_04520"
     CDS             1883..>2118
                     /locus_tag="VFB89_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26594.1"
                     /translation="MTPGTPLTDRPAVAEPERSTWWDDERAFVGSRPTWSLIVLAWLI
                     PASVGLLQMVAANLLRGTLGAEWPWALLQIPRWLT"
BASE COUNT          357 a          739 c          653 g          369 t
ORIGIN      
        1 tcaatcgggg tgagactttc aaggggtcag agcccttgaa ttcaaggagc tcggcttcat
       61 aaagatctcg aattcaaggg ctctgacccc ttgaaagttc gactccgacc ctgaaccgcc
      121 gcctaccccc aatttccgcc actcgccact ccatcattct cgcctgacca gacccgctac
      181 acgttggcgg ctccacccac caccgtgaat ggagttgtca tgtcgatatt ccgaaagctg
      241 cgcctgctga gcgccggagc gctcgccctc gcgacggcga gtgccttttt cgcgcaacct
      301 gctacggcag cgcaccgcaa gtcagcagcc gcggcagcgc tcgacaccgc catcgcccgc
      361 atgggcggcc aggagaccct cgcgcacatc gagcgcgtac gcttcgagat gatgaccctt
      421 tggcagcgca tgacgttcga cacccgtccg acggacctca tcagcacata tgagctgcac
      481 agcgaccttc ggaactatcc actcgtcgcc tggcgcaaca cgcgccgatt cgtcagcggc
      541 ccccagatgc aggagatgac cgacatcgtc cagaaggacg cgggcgtccg gcgcttcgcg
      601 ccgaaacccg atgggacggc gtcgccgtgg gcaccgatca acatcgccta catcgacgag
      661 cggaaagagg tcttcgcgtt cgcccccgaa cggttgctgc tcgctgccca tgccgcgagc
      721 gatctgcgcg agctctccga cacgacgatc gccggacagc cccacacgcg gctgagcgcg
      781 acgatcgacg ggttccccac gaccatcttc ctccggcgca acgacggctt cctcgccatg
      841 gcccggtatc gtgcgggcca gcctaacgat ttcgggctcg cgccgtgggg agacatggag
      901 acggagatgt ggtactcccg ctggacgaag ttcccggtcc cgggcacacc cggcgtcgct
      961 tatcccacgc aggtggacgt ccgccgagtc ggccgcatgt acaagcgcct cgtcgtgctc
     1021 tccgccaact tcgacgccgc cgcacccgcc gattcgtttg ccatctcgga ctccttgcgg
     1081 accgcgttcg agagcggtcc gtccagcaaa ccgatgtggg aggttccgat ggactcggcg
     1141 aagctgctcg agccgcggct ggcgcgcctc ggcaaccccg gccagacgca ggccgccgtg
     1201 aagctcggca agtcgtggct cttcctcgag ggcgcgagcg tgccgcagcg gagcgagacc
     1261 gatgtccgat ggctcgagtc ggcggacccg gggtcgacca ttgccggact cctcgtgacg
     1321 gccccgaaca cgggtcgcgg cggggctgcc tggtttaccc agcggaagct tcccgtctat
     1381 gtgacgccgg gcgctgtctc ggcgatgggc acgacgctcg cgaactggaa acagccacct
     1441 tctgctgtaa aggtcgtgtc gaaatcgcag tggctgcgca tcagcggtga ttccgtatgg
     1501 gtcgagccga tcgacctgcc ggactatccc ggcgcgctcg tagcgtacgt gccgtcgctg
     1561 cgctgggtgt acagtggacc agcggcgacg ccgctctact tcgacatggt cgtcgcccgc
     1621 attcgggacc gcgggtgggc agtcgaccgc atcgggtcgg tccggtcgct cacgcaaccg
     1681 atcccggccc ggaccgcgtc gcgttaggca gtggccgaac tgcctttaac cgccgaggac
     1741 gcagaggatc gcagagaact acccaaggat tttctctgcg gtcctctgcg tcctcggcgg
     1801 ttaaatcgga ggtctctgat tttcaattca acctctgtga ttcaaccggc ggtagcggct
     1861 cagacgccgt agattccatc cgatgacacc cggcacgcca ctcaccgatc gtccggcagt
     1921 cgccgagccc gaacgctcga cttggtggga cgacgagcgc gcgttcgtcg gctcgagacc
     1981 cacgtggtcg ctcatcgtgc tcgcgtggct catcccggcg tcggtaggac tgctccagat
     2041 ggtcgccgcc aatctgctcc gcggcactct cggcgcggag tggccgtggg cgctcctgca
     2101 gatcccgcgc tggctcac
//
LOCUS       DASRJQ010000198         4452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1840780, whole genome shotgun sequence.
ACCESSION   DASRJQ010000198 DASRJQ010000000
VERSION     DASRJQ010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1840780"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VFB89_04525"
     CDS             <1..715
                     /locus_tag="VFB89_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26595.1"
                     /translation="WEEALWDYRMQGTMAFERFRNPAGTSFLSSSDLGFGVDGKFKYD
                     FVNFQIGAYNGEFYSGPERDEHKDVEGRVSIRVLESDDHSRVGGLRITGYGAIGTPTG
                     GGKRNRYIGMVSYKSKLFTLAGEFGGTRDRLDQGPPVTGNPAPVPQTDGTILSFFGVF
                     NVPNSKAAFIARWDAQNPNKDVDNDKLYRFIGGVSYQLTPEVRLLGDVDNLWSESGVY
                     TNAVNSTRSSALFQMQIAF"
     gene            757..1812
                     /gene="pstS"
                     /locus_tag="VFB89_04530"
     CDS             757..1812
                     /gene="pstS"
                     /locus_tag="VFB89_04530"
                     /inference="COORDINATES: protein motif:HMM:NF008171.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HZM26596.1"
                     /translation="MTGKLSALVLTLGGTALLGVGPLAAQTSLTGAGATFPNPIYSKW
                     FAEYNKKTGVQINYQSIGSGGGIKQYTEGTVDFGASDGPMSEEQMQAVGGHVLHIPTV
                     LGAVVVTWNLPALGATVLKFDGPTIADIFLGKVTKWNDKRLAALNPGVKLPDQDIIVV
                     HRSDGSGTSYIFTDYLSKLSDEWKNKVGKATSVNWPVGLGGKGNEGVTQQVKQTEGSL
                     GYVELIYAEANKLPYAEVKNASGQFVKPSLESVTQAAGSAKFDKNTDFRVSITNAPGQ
                     GAYPISSFTWLLVRPDMKDAAKAKAMKGFLDWMVTPEAQSMATQLVYAPLPKEVVELV
                     KERLKTLKGAGKALAGA"
     gene            1899..2267
                     /locus_tag="VFB89_04535"
     CDS             1899..2267
                     /locus_tag="VFB89_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26597.1"
                     /translation="MRLDELALVILAACGGGDQRTPAANGQPAKAPPSGTGLAGVKQT
                     EGALGYVELIYASANELPYAELKNAAGQFVVPSLESVTQAAGGAQFAPNTDFRVSIPT
                     RRGRIPIRSPPSVGCSSGRQ"
     gene            2264..2425
                     /locus_tag="VFB89_04540"
     CDS             2264..2425
                     /locus_tag="VFB89_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26598.1"
                     /translation="MTDSARAKALTGFLEWMITPEAQDMAAQLVYAPLPKQVITLVEE
                     RIAQLGIAH"
     gene            2520..3518
                     /gene="pstC"
                     /locus_tag="VFB89_04545"
     CDS             2520..3518
                     /gene="pstC"
                     /locus_tag="VFB89_04545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HZM26599.1"
                     /translation="MTMATSAAPSQRSPGRPKISASLQGVAYGDHAFKLVLTIAALLI
                     PILLGFLLLELWQGSRLAMSRFGLDFIRTSVWDPVAEQFGAFPLVFGTLLSSLLALLI
                     AVPLSLGVAIYLTEFAPKFLRQPVAFTIGLLAAIPSVVYGLWGIFVLIPLLRKTAFPL
                     LRSALGFLPFFQGPIYGPSMLAASVILAIMVMPYIMSVSREVLLAVPNSQREAALALG
                     ATRWEAVITAVLPYARSGVIGAIILGLGRALGETMAVTMLIGNRHEIAASLFAPGYTM
                     AAVIANEFSEAATDMHFSALTYVAFVLFVLTVAVNAGARLLIWRVARGSAVGSKAL"
     gene            3515..4360
                     /gene="pstA"
                     /locus_tag="VFB89_04550"
     CDS             3515..4360
                     /gene="pstA"
                     /locus_tag="VFB89_04550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HZM26600.1"
                     /translation="MNRRLIRRVASNAMVGLMIVAVILAVLPLLFILLNLVAKGASSL
                     SWDFFTKMPAPPGESGGGVANAIVGTLIIIGAASLIGIPVGVGAGIYCAEYPGSRLTW
                     LTRFVADVLNGTPSIVVGVFAWTWIVAQQKHFSALAGSVALAMLMIPMVMRTTEEMIK
                     LVPNSLREAALALGYPRWRTSLAVVVRTTMPGIVTGTLLAVARIAGETAPLLFTALGS
                     QYLSFKVNQPMAALPLTVFTYATGPYEEWHRYAWAAALVLIFVVFLLGIGARLAIGRS
                     QISNG"
BASE COUNT          777 a         1395 c         1412 g          868 t
ORIGIN      
        1 ctgggaggaa gcgctctggg actaccggat gcagggaacg atggcgttcg agcggttccg
       61 caatccggcc ggcaccagct tcctctcgtc ctcggatctc gggttcggag tcgacggcaa
      121 gttcaagtat gacttcgtga actttcagat tggcgcttat aacggcgagt tctacagcgg
      181 tcccgaacgc gatgaacaca aggacgtcga ggggcgggtc tcgattcgcg tgctcgagag
      241 cgacgaccac agtcgagtcg gcggcctccg gataaccggc tacggcgcga tcgggacccc
      301 cacgggtggc gggaaacgga accgatacat cggaatggtc tcatacaagt ccaagctctt
      361 cactctggcg ggcgagtttg gggggacgcg ggatcgcctt gaccaggggc cgccggtcac
      421 cggcaacccg gctccagtcc cgcaaaccga cggcacaatc ttgagtttct tcggcgtgtt
      481 caacgtgcca aattcgaagg ctgcatttat tgctcgctgg gacgcgcaga acccgaacaa
      541 ggacgtcgac aacgacaaac tgtaccgatt catcgggggc gtgtcctatc agctcacacc
      601 cgaggtccgg ttactagggg acgttgacaa tctctggtcg gaatcaggcg tgtacactaa
      661 cgcggtgaac tccacccgca gctccgcctt gttccagatg caaatcgctt tctagcgcga
      721 ggagcagttg cccattctca atgaaggagc ggtacgatga caggcaagct ttcggctctg
      781 gtcctgactc tcggaggaac tgccctcctc ggcgtggggc cgctggcggc gcagacctcc
      841 ctgacgggtg ccggggcaac gttccccaat ccgatctact cgaagtggtt cgccgagtac
      901 aacaagaaga ccggggttca gatcaactat cagtcgatcg gctccggcgg cggaatcaag
      961 cagtacaccg agggaaccgt ggacttcggg gcctcggatg gaccaatgag cgaagagcag
     1021 atgcaggccg tcggtggtca tgtgctgcac atcccaaccg tgttgggtgc ggtcgtggtt
     1081 acctggaacc ttcccgctct cggcgcgacc gtgctcaaat tcgacgggcc gacgatcgcg
     1141 gacattttcc tgggcaaggt gaccaagtgg aatgacaagc gcctggctgc gctcaacccg
     1201 ggcgtcaagc ttcccgacca ggacatcatc gtggtgcatc gctccgacgg ctcgggcacg
     1261 tcgtacatct tcacggacta tttgagcaag ctttccgacg agtggaagaa caaggtcggc
     1321 aaagccacct cggtgaactg gccggtcggt ctgggtggca aaggcaatga aggcgtgacc
     1381 cagcaggtga aacagaccga gggctccctg ggctacgtcg agctcattta cgctgaggcg
     1441 aacaaactgc cctacgcgga ggtgaagaac gcgtccgggc aattcgtgaa gccttccctg
     1501 gagtcggtga cccaggccgc gggcagcgcc aagttcgaca agaacaccga tttccgggtg
     1561 tcgatcacta atgcgcccgg ccagggcgcg tatccgatct cgtcgtttac ctggcttttg
     1621 gtccgccccg acatgaagga cgccgccaag gccaaggcca tgaaggggtt cctggactgg
     1681 atggtcacgc cggaagccca gtccatggcg acccagctgg tatacgctcc actgcccaag
     1741 gaagtggtcg agctggtgaa ggagcggctc aagacgctca agggagcagg taaggcgctc
     1801 gccggcgcct gaagcgcccg gctccggtac tcttcaggtc actgagtcga ttcgatcctg
     1861 gcctggaggc gtcgcatgtg tagcgggcgt ttgttcaagt gcggcttgac gagctcgcgc
     1921 tggtcatcct cgccgcctgc ggtgggggcg accagcgcac tccggctgca aacgggcagc
     1981 ccgcgaaggc tcctccttcc ggcaccgggc tcgccggcgt gaaacagacc gaaggagcac
     2041 tcggatacgt cgagttgatc tacgcatccg caaacgagct cccgtacgcc gagctcaaga
     2101 acgcggccgg gcagttcgtt gtaccctcac tcgagtcggt cacccaggct gcgggcgggg
     2161 cgcagttcgc gccgaacacc gattttcggg tgtccatacc aacgcgccgg ggcagaattc
     2221 ctatccgatc tcctccttca gttggctgct catccggccg acaatgaccg attcggcccg
     2281 ggccaaggca ctgacgggat ttctggagtg gatgatcact ccggaggccc aggatatggc
     2341 ggcccaactg gtctacgcgc cgctgccgaa gcaggttatc accttggtcg aggagcggat
     2401 cgcccagctt ggcatagcgc actagggccc ggctcgaggc gccgcgatgt gggccagccg
     2461 tgacgagtcc gttacgattt cgtgaccgtt agccggtttc ccgcctctaa actcccgcca
     2521 tgaccatggc aacttctgcc gctccatccc agcggagccc gggccgtccg aagatctccg
     2581 cctctctcca aggcgtggcc tacggcgatc acgcgttcaa gctggtcctt acgatcgcgg
     2641 ccttgctcat ccctattctg ctgggattcc tcctgctcga actctggcag ggttcccgcc
     2701 tcgccatgtc cagattcggg ctcgacttca ttcgaaccag cgtctgggac ccggtggccg
     2761 agcagttcgg cgcctttccg ctggtattcg ggaccctgct gtcctccttg ctcgcgctgc
     2821 tgatcgcggt cccgttgtcg ctcggcgtgg ccatctacct cacggagttc gcgcccaagt
     2881 tcttgcgcca gcctgtagca ttcaccatcg ggctcctggc agcgattcca agcgtggtgt
     2941 acggactgtg gggcatcttc gtgctgattc cgctgctccg gaaaacagcc ttcccgctgc
     3001 tccgaagcgc tcttggcttc ctcccgttct tccagggacc gatctacggc ccgtccatgc
     3061 tggcggccag cgtgatcctc gcgatcatgg tcatgccgta catcatgtcc gtctcgcggg
     3121 aggtgctcct cgccgtgccc aacagccagc gagaggctgc actcgcgctc ggagccacgc
     3181 gctgggaggc cgttatcacg gcggtgctgc cgtacgcccg gtcgggagtc atcggcgcaa
     3241 tcattctggg cctgggccgc gccctgggcg aaacgatggc ggtgaccatg ctgatcggaa
     3301 atcggcatga aatagccgct tcgctgttcg cgccgggtta cacaatggcg gccgtgatcg
     3361 cgaacgagtt ttccgaagcc gcgaccgata tgcacttctc ggcgctcacc tacgtcgcgt
     3421 ttgtgctgtt cgtgttgacc gtggcggtga atgccggcgc gcgcctgctc atctggcggg
     3481 tggcccgggg ctcggccgtg gggagtaagg cgctgtgaac cgccggctca tccggcgggt
     3541 cgccagcaat gcgatggtcg gtttgatgat cgtcgcagtg atcctggcag tgcttccatt
     3601 gctcttcatt ctgctcaatc tcgtcgccaa aggggcgagc agcctctcct gggacttctt
     3661 cacgaagatg ccagcccctc cgggtgagag cggcggcggc gtggccaatg caattgtggg
     3721 cacgctgatc atcatcggcg ccgcgagcct gattggtatc ccggtggggg tgggcgcggg
     3781 gatatactgc gccgagtacc ccgggagccg cctcacctgg ctgacccgtt tcgtggccga
     3841 cgtcctcaac ggcaccccgt cgatcgtggt gggcgtgttc gcgtggacct ggatcgtcgc
     3901 gcagcagaag catttttcgg cgctggcggg aagcgttgca ttggcgatgc tcatgatccc
     3961 gatggtgatg cggaccaccg aggagatgat caagctggtg cccaattcgt tgcgcgaagc
     4021 agcgctcgcg ctaggctatc cccgctggcg caccagcctg gcggtcgtgg tccgcaccac
     4081 gatgccaggg atcgtgaccg ggacactgct tgcggtggcg cgtatcgcgg gtgaaaccgc
     4141 accgctgctc ttcacggcac tcgggagtca gtatctgagc ttcaaggtca accagcccat
     4201 ggccgcgctt cccctgaccg tgttcaccta cgccaccggt ccctatgagg agtggcaccg
     4261 ctacgcctgg gccgcggcgc tcgtgctgat cttcgtggtg ttcctgctcg gcatcggcgc
     4321 tcggctcgcg atcggccgga gtcagatctc gaatggctga gctccgggcc cggatcgaga
     4381 ccgacaagct caccgccttg tacggcaagg tcactgcggt gaagaatgtg acgttgtcgt
     4441 ttgcgcccaa tc
//
LOCUS       DASRJQ010000199         2325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3903585, whole genome shotgun sequence.
ACCESSION   DASRJQ010000199 DASRJQ010000000
VERSION     DASRJQ010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3903585"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2325
                     /locus_tag="VFB89_04555"
     CDS             <1..>2325
                     /locus_tag="VFB89_04555"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26601.1"
                     /translation="PAVTPGSGFTEIAEPSSGESNTLEAEWAVNLTNVSASWGSAKNA
                     GLLGIEIKAAGGSGQPPLEPSPIDPTVATTLGTTTEFLYTGSNPVQTGVAPGTIVPER
                     AAVLRGKVLASDGQPLSGVGVTILGHSEFGSTVNRADGMFDMVVNGGGLLTVEYAKAG
                     YLRAQRSVDVPWQDYSLIDDVVLVALDPAVTMIDFSSPIQVARGGVASDLDGTRQATL
                     LFSQGTQATMVLADGSTQPLSTLAVRATEFTIGPTGTQAMPAELPPTSAYTYAVEYSV
                     DSALAVGAREVQFSQPVFTYVENFLSIPVGLDVPAAYYDRRLGQWVSAMDGRVIKILG
                     ITAGRADLDVTGSGQVSDPATLALLGVTDDERTQLALLYGPGQTLWRVPITHFTSYDY
                     NYPYTAGPDAPPPPPGGPQKPKSPDDPNCKKSSIIECETQVLREQIAITGTPFSLNYT
                     ARRVAGYRAGRALRIPLSGPTVSAGLKRIILSVEVAGKSVVDTFPAAANQTAEFTWDG
                     TDAYGRTVPGVVVATIRIGYLYRAVYTEPAAFAEAFAVPSGVAVEPIGGGEGSTIAVR
                     NGSDTASQGILISRQFRTTLGPWLSSPSAGIGAWTLSVHHGYDPVDRTIHLGDGTQRS
                     AANLALVIQTVAGSGAGGCITCDLGEGVPATQGIVGSPFGVAAAPDGGFYVADQLWGR
                     VRRVGPDGVMTTFAGSGNSGGFSGDGGPATAAKLSQPTGIALGPDGTLYIADFGNQRV
                     RRVAPDGVITTGAGTGTFGFNGDDRLATTAQLSNP"
BASE COUNT          414 a          712 c          785 g          414 t
ORIGIN      
        1 cccgcagtca cacccgggag cgggttcacg gagattgccg agccgtcgtc gggcgagagc
       61 aatacgcttg aggcggaatg ggcggtgaac ctcaccaacg tctcggcgag ctggggatcg
      121 gccaagaatg ctgggctcct ggggatcgag atcaaagctg cgggcggtag cggtcagccg
      181 cccctcgagc cctcaccgat tgatccgaca gtagccacga cgctcggcac tacaaccgag
      241 ttcctctaca cggggtcgaa ccccgttcag actggcgtcg cgccggggac gattgtgcct
      301 gagcgagcgg cggtactgcg gggaaaggtg ttggcgagtg acggacagcc gttgtccgga
      361 gtgggagtca ccatccttgg acattcggaa ttcgggtcca cggtgaatcg ggcggatggg
      421 atgttcgata tggtggtgaa tggcggtggg ctgctcacgg tggagtacgc gaaagccggc
      481 taccttcgcg cgcagcgcag cgtggacgtg ccatggcagg actactcgct tatagacgac
      541 gtcgtgctcg tcgcgctgga ccccgcggtg accatgatcg atttctcttc gccgatccag
      601 gtggcgcgag gcggcgtcgc atctgacctg gatggcaccc gtcaggccac actgctattc
      661 agtcagggca cgcaggcgac gatggtgctc gccgacggca gcacccagcc gctcagtacc
      721 ttggccgttc gtgcgaccga gttcacgatc ggccccaccg gcactcaggc gatgccggcg
      781 gagctgccac cgacgagcgc ctacacttac gcggtcgagt atagcgtcga ttcggcgctg
      841 gctgtgggtg cgcgcgaagt tcagttcagc caaccggtgt ttacctatgt cgagaacttt
      901 ctcagcattc cggtgggact cgacgttccg gcggcgtatt acgaccgtag gcttgggcaa
      961 tgggtctccg cgatggacgg tcgagtcatc aagatcctgg ggattacagc cgggcgcgcc
     1021 gacttggacg tgacgggcag cggccaggta agtgatcctg ccaccttggc tttgctcggc
     1081 gtgaccgatg atgagcgcac gcagctcgca ctgctctatg gcccgggtca gacgctgtgg
     1141 cgggtaccca taacccactt cacgtcctat gactacaact atccatacac cgctgggcct
     1201 gacgcccctc ccccgccccc aggcggcccg cagaagccga agtcgccgga cgacccgaat
     1261 tgcaagaaga gctcgatcat cgagtgcgag actcaagtgc tccgcgagca gattgcgatt
     1321 acgggaacgc cgttctcact caactacacg gctcgtcgcg tggccggata cagagccggt
     1381 cgagccctca gaatcccgct aagtgggccg accgtttcag caggcctcaa gcggataatt
     1441 ctatctgtgg aggtagccgg taagagcgtg gtcgatacct tcccagctgc ggcgaaccag
     1501 accgcagagt tcacctggga cggaaccgac gcgtacggca gaaccgtacc cggtgtcgtc
     1561 gtcgcgacga ttaggatcgg gtatctgtat cgagccgtgt acacggagcc ggccgccttc
     1621 gcggaggctt tcgccgtgcc ttccggggta gcggtagagc ccattggcgg aggcgagggc
     1681 tcaacgatcg ccgtgcgaaa tggatcagac accgcatccc agggcatcct gatttcgcgg
     1741 cagttcagaa cgacgctcgg accctggctc tcgagcccga gtgccggtat cggtgcctgg
     1801 acgctcagcg tgcatcacgg ctacgatccc gtggacagga cgattcatct gggggatgga
     1861 acccagcgca gcgcagcgaa tctcgcgctc gtcatccaga ccgttgcggg ctcgggcgcc
     1921 ggtggctgca taacctgcga ccttggcgaa ggagtgcccg cgacacaagg catcgtgggg
     1981 agtccgttcg gcgtggccgc ggcgccggat ggcggtttct atgttgccga ccaactgtgg
     2041 ggccgggttc ggcgcgtggg accggatggg gtgatgacga cctttgcggg gtctgggaat
     2101 agtggtggct tcagcggtga cgggggcccc gccaccgcgg cgaaactatc gcagccgacc
     2161 ggaatcgccc tcgggcccga tgggaccctc tacattgcgg acttcgggaa ccagcgcgtg
     2221 cgtcgggtgg cgcctgatgg ggtcatcacc actggggccg ggaccggcac gtttggcttc
     2281 aacggggatg atcggctggc caccaccgcc cagctgtcca accct
//
LOCUS       DASRJQ010000200         2020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_4217150, whole genome shotgun sequence.
ACCESSION   DASRJQ010000200 DASRJQ010000000
VERSION     DASRJQ010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4217150"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VFB89_04560"
     CDS             <1..467
                     /locus_tag="VFB89_04560"
                     /inference="COORDINATES: protein motif:HMM:NF015369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZM26602.1"
                     /translation="TVLARASDNYFQSQAYRMQRERNSERPRDVSAGLAMNGVPLNSV
                     ILDPAPEQTVPAGSVRVRGWAMGSEARPLTTIELSADGGRHWVPARITRAGGPWTWSF
                     WEATVALAPGSHTLAARATDSSGATQPSTVDETWNVKGYGNNAWHRVTITAR"
     gene            569..>2020
                     /locus_tag="VFB89_04565"
     CDS             569..>2020
                     /locus_tag="VFB89_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZM26603.1"
                     /translation="MTATQIDPVLTDRVRAASALLESLVADRGLLALVPREDRRRLLD
                     AAANVYHPDAKARRLLVKATVRERKRARIRKDEEVLAQTGIRTLRRQTVFTTPNVFPP
                     EQQASLETPESHVSRLTTHDTRNCYICKQDYTHVHHFYDKLCPACGELNFAKRTELAD
                     LTGRVALLTGGRVKIGYQAGLKLLRAGAHLIVTTRFPRDSALRYTMEPDFADWGDRLE
                     IFGLDLRHTPSVEAFCRQLLETHDRLDFIVNNACQTVRRPPEFYAHMMAVETAAQATL
                     PPKARRLLGAYEGLRGYHMLPGSEPAFRPFAAAQGDKLGLTHSAALSQVPLLPEDHAG
                     GNLLFPEGKLDQDLQQVDLRGRNSWRLMMDEVSSVELMEVQLVNAIAPFVLNARLKPL
                     MLRTPNRDKHIVNVSAVEGQFYRKFKTTRHPHTNMAKAALNMMTRTSATDYFNDGIHM
                     NSVDTGWVTDEDPIEIALRKTAEQRFHPPLDAVD"
BASE COUNT          364 a          746 c          585 g          325 t
ORIGIN      
        1 tcaccgtcct cgccagggct tccgacaatt atttccagtc ccaggcctat cggatgcagc
       61 gcgagcggaa ttcggaacgg ccgcgcgacg tctccgccgg cctggcgatg aacggggtcc
      121 cgctcaattc cgtcatcctc gacccggccc ctgagcagac cgttccggcc ggctcggtgc
      181 gggtccgcgg ctgggcgatg ggatcggagg cccgccccct caccacgatc gagctgtcag
      241 ccgacggcgg ccggcactgg gttccggcac gcataacccg cgcgggcgga ccctggacct
      301 ggagtttctg ggaggcgacg gttgcgctgg cgccgggcag ccacaccctg gccgcccggg
      361 ccaccgacag cagcggcgcc acccaaccct ccaccgtcga cgagacctgg aacgtcaaag
      421 gctatggcaa caacgcctgg catcgcgtca ccatcaccgc acgctgagac ccgcccccat
      481 gcgcgtcatc cgcgtttgct tttccagttt ttcctgaacg taacaatgaa tcttgcagga
      541 gccgcccggc ctattaccct tccgccccat gacagccaca caaatcgacc ccgtcctcac
      601 cgaccgggtc cgcgccgctt cggcgctgct cgaatcactg gtcgccgacc ggggcctgct
      661 cgcactggtg cctcgagagg accggcgccg cctcctcgat gccgcggcca atgtctatca
      721 tcccgacgcc aaggcccggc gactcctggt caaggccacc gtccgcgagc ggaagcgcgc
      781 ccggatccgg aaagacgaag aggtcctggc gcagaccgga atccgcacac tgcgccggca
      841 gacggtgttc accacgccga acgtcttccc cccggagcag caagcttcat tggagacgcc
      901 cgagtctcac gtctcacgtc tcacgactca cgacacccgt aactgctaca tttgcaaaca
      961 ggattacacg cacgtccacc acttctacga caagctctgc cccgcctgcg gcgagctgaa
     1021 cttcgccaaa cgcaccgagc tggccgatct caccggccga gtcgcgctgc tcaccggcgg
     1081 ccgcgtcaag atcggctacc aggccggact caagctgctc cgtgccggcg cgcacctcat
     1141 tgtcaccacc cgctttcccc gcgattcggc cttgcgctac accatggagc ccgattttgc
     1201 cgactggggc gaccggctcg agatcttcgg gctcgacctc cgtcatactc cgagcgtgga
     1261 ggcgttctgc cgccagctcc tcgagaccca cgaccgtctc gacttcatcg tcaacaacgc
     1321 ctgccagacg gtgcgccggc cgcccgagtt ctacgcacac atgatggcgg tggagaccgc
     1381 ggcccaggcc acgcttccgc ccaaggcacg ccgactgctc ggcgcctacg aagggctgcg
     1441 cgggtaccac atgctgccgg ggtcggaacc ggccttccgc ccattcgctg ccgctcaggg
     1501 tgacaagctc ggactgactc actcggcagc gctctctcag gtgccgctgc ttccggaaga
     1561 tcatgccggc gggaacctgc ttttccccga gggcaagctc gatcaggact tgcagcaagt
     1621 ggatctgcga gggcgcaatt cctggcggct catgatggat gaagtgtcgt cggtggagct
     1681 gatggaggtc cagctcgtca acgcgatcgc gccgttcgtg ctgaacgcga ggctcaagcc
     1741 gctgatgctg cgcaccccga atcgcgacaa gcacatcgtc aacgtgtccg cggtcgaggg
     1801 gcagttctat cgcaaattca agacgacccg gcatccccat accaacatgg ccaaggcggc
     1861 gctcaacatg atgacccgca cgagcgccac cgactacttc aacgacggca ttcacatgaa
     1921 cagcgtcgat accggctggg tcaccgacga ggaccccatc gagatcgcgt tgcgcaagac
     1981 cgccgagcag cgcttccatc ccccgctcga cgccgtcgat
//
LOCUS       DASRJQ010000201         5688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_434005, whole genome shotgun sequence.
ACCESSION   DASRJQ010000201 DASRJQ010000000
VERSION     DASRJQ010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434005"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            448..1731
                     /locus_tag="VFB89_04570"
     CDS             448..1731
                     /locus_tag="VFB89_04570"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HZM26604.1"
                     /translation="MLFARTHAGPPSCLPRRLSALSWTVATTCLFAACGGSDLTLPSG
                     SLPAEIRVVDGDAQSGSVGQLLPAPIVVEVRDARGQEVEGTSVEFALTSAGTGAEIVP
                     STATTDARGRAQVRMLLGDKAGLQVGEARVVGAGAAAPKTTFSAMAVSPGPGNGGPPP
                     AQSNKSPQAAFEVSCQDLTCTFTDQSSDDDGSVVSWQWNFGDQATSTQRNPSHSYQTA
                     GQFAVLLTVRDNAGAANTTAHQADPSAPASPPPTNKPPQAEFEVHCSDLRCTFTDRSK
                     DDDGTIVSWRWDFGDGGTSTERNPSHSYRTAGRYQVRLTVTDNDGAGASKTHEADPSA
                     PPPPPPPPPPANKPPEADFDIHCKHLTCTFADRSKDDDGTIVAWHWSFGDGDDSNERN
                     PAHTYDDPGHYDVSLTVTDNDGATGTKTRRADVKE"
     gene            complement(1744..2733)
                     /locus_tag="VFB89_04575"
     CDS             complement(1744..2733)
                     /locus_tag="VFB89_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26605.1"
                     /translation="MTEEVSKPRKLRPIPFGERPGKEPYNASEVVPLGDSRFLFCDNN
                     VNDAFFELRLAPDGSMACPLVRRPIRGIEPGTIDDMEGLESATLAGRTYLFATSSFSL
                     KPRKKHHKKKGQRGKLAADRESVLRLAIDDDEQLVAESIPDFRSWLIEQAPELTKPAK
                     YLPDDGGLNVEALSWDPNNQALLFGLRTPVADGKPLVVRVRIKQFDGPWDLSNLEILS
                     PIVLPIETSGRRREGLRAMTYDPANSAVLLTVGRRIAGSKRRFELYSWDGQQEGLLKN
                     FRQVRFDRRMRVEGVAHGTITGRGAVVFVDDRGGYQLLWDDDPRLAVTEQLSW"
     gene            complement(2730..3767)
                     /locus_tag="VFB89_04580"
     CDS             complement(2730..3767)
                     /locus_tag="VFB89_04580"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HZM26606.1"
                     /translation="MTNVRQAVAACLAAGLATLSPVGRPPVHSADQSEKISGYAEFKR
                     GDTVMVDGQRVVATPATRVSGAKTLKDIPLGYEVKVKGSRDKSGALIAAQVDTRPNGT
                     DGTEQEIIAASNQAEDTWVKHKMMFEPADSGKIIKIGEILESGPYVDRARKIIDKLRP
                     SYVPASALRVRAVKTDQWNASAMANGAIWVYTGLMDAMSDEEMAIVLGHELTHYTHEH
                     VRRQQSKGTLAQILGVGASIALTQISNPTAQQVAAFGGQLGLSALMSGYSREFEDQAD
                     RVGLRYIYEAGFDPTKGPGLWEKFKQKYGEEDKLTNFFTGDHSRPTERIRNIQRQIQL
                     NNYTTPLKKTS"
     gene            complement(3962..5395)
                     /locus_tag="VFB89_04585"
     CDS             complement(3962..5395)
                     /locus_tag="VFB89_04585"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HZM26607.1"
                     /translation="MSAIREERRVRDRRSAAVTKPAKEIAGTPPRTVMTSAYVETVGR
                     LAYIWAWPLVNGHNRAAAFEQLPEPGHIGGILPAAPPGYVGMLTDYIEESERFVTCPN
                     QDTVYGAGFQRLDSRPVIVQVPDFGDRFWVYQICDARTDAFCAVGKQHGTKPGFYLLV
                     GPNWNEQVPAGVTAVYRSSTDLAAVFPRVFQDDTPEDRAAIQPLLRQIMVYPLSEFDG
                     KMKTKDWTKVPSFPMATGGKGETKWVIPEKFFDQLPDVMKKVPPLPGEESLYTLFQSV
                     LDAAANDPKLTQTLTETAIAAERDLIGPLFQFHNNGRPIGNGWTSPPNGARWGVDYLS
                     RAATARSNMYDNAPEETRYIYTDFDSGGERLNGAHAYTATFPKGETPPVNGFWSLTLY
                     NEDHLFSPNPLKRYSLGTKNAKSLKLGTDGSLPLYIQNESPGTDKEANWLPAPKHADF
                     SLYIRSYWPKPAILDEKWTPPAIRRTR"
BASE COUNT          985 a         1817 c         1781 g         1105 t
ORIGIN      
        1 ccgtggttat aggcgctgac attgaaccag ccaatgtcgt cgccccagat gatgagaatg
       61 ttcggcttgt ccgtcttcgc tgctgccgct ctctttgcca tagcccctcc ctccgcctgc
      121 ggtctcaggt accaaggaac ctcgagccgg cagatgccgg cgcctgagcc agcctaaagc
      181 gctgacgcga gactggtaac tgtcaattgt gacaggcgtt gcgcgggagc cgagcggctc
      241 accgaaacag cgcaggggat cggaatcccg gcgcaggctg caacgatcgc tatgaacctg
      301 ccggcaagcg tactcgcgcc gactttgacc gcgcctgtgc ggtccatcac ctcgaatagc
      361 ctgatcgaac accagtttta aatccgctat ttgatccggt tgcccctaca gagtgccttc
      421 gaacggcatc tgctctgagg gttgaacgtg ctcttcgccc gcacccacgc tggacccccg
      481 tcctgcctgc ctcgccggct gagtgcccta tcgtggacgg ttgccacaac ctgcctcttc
      541 gctgcgtgcg gagggtcgga cctgacccta cccagcggca gcctgccggc cgagatccgt
      601 gtggtggacg gggacgctca aagcgggtcg gtgggacagt tgcttccagc gcccattgtc
      661 gtcgaggtaa gggacgctcg gggtcaggaa gtcgaaggga caagtgtgga attcgcgctt
      721 acgtctgctg gcaccggagc cgagatcgtt ccttcgactg cgaccacgga tgcgaggggg
      781 cgcgcgcagg tccgcatgct ccttggcgac aaggccggcc tccaggtcgg ggaggcgcgg
      841 gtggtgggcg ccggcgcagc cgcacccaag accaccttca gtgcgatggc cgtttcaccg
      901 gggccaggca acggcgggcc acctccagcg cagagcaaca agtcacccca ggccgcattc
      961 gaggtgagct gccaggattt gacctgcacg ttcactgacc agagcagcga cgacgatggc
     1021 agcgtcgtga gctggcaatg gaactttggg gaccaggcca cgtccaccca acggaatccc
     1081 tcgcattcgt atcagacagc cgggcagttc gcagtccttc ttaccgtgag ggacaatgcc
     1141 ggcgcagcca acaccaccgc ccatcaggcg gatcccagcg caccggcgtc accgccgccg
     1201 acgaacaaac cgccccaggc ggaattcgag gtgcattgca gcgacctgag gtgcacgttc
     1261 accgaccgaa gcaaggacga tgacggcacg attgtgagct ggcggtggga ctttggcgac
     1321 ggaggcacat ccaccgagcg gaatccttct cattcatacc ggacggccgg ccgctatcag
     1381 gtccgtctta ccgtgacgga caacgatggg gccggcgcca gcaagactca cgaggctgac
     1441 cccagcgcgc cgccgccgcc acctcctcca cctccgccgg cgaacaagcc gcccgaggcc
     1501 gacttcgaca tacactgcaa gcacctgacc tgcacgtttg ccgaccggag caaggacgac
     1561 gacgggacca tagtcgcctg gcactggagc ttcggggacg gcgacgactc aaacgagcgg
     1621 aacccggcgc atacgtacga cgacccggga cactacgacg tttccctcac cgtcaccgac
     1681 aacgacggcg cgacaggcac caagacccgc cgcgccgacg taaaggaatg aggaggcctt
     1741 cgactaccag gacagctgtt cggtgactgc cagacgcggg tcgtcgtccc acaacagctg
     1801 atatccaccg cggtcgtcca cgaagacgac agcgccccgg ccggtgatgg tcccatgggc
     1861 tacgccttcg actctcatcc ggcggtcaaa ccggacctga cggaagttct tgaggagtcc
     1921 ttcctgctgt ccgtcccagg aatacagctc gaaccgccgc ttggacccgg caattctgcg
     1981 gccgaccgtg agcagcacgg cgctgttagc tggatcgtag gtcatggcgc ggaggccctc
     2041 gcgccgccga ccgctcgtct cgatgggaag cacgatgggc gacaggatct cgagattgct
     2101 taggtcccag ggtccgtcga actgcttgat ccgtactcgg acaacgagcg gctttccgtc
     2161 ggcgacaggt gtgcgaagcc cgaagagcag ggcctgattg ttgggatccc agctcaaggc
     2221 ttcaacgttc agcccgccgt cgtcaggcag gtacttcgct ggcttggtca gctccggtgc
     2281 ctgctcgatc agccaggacc tgaaatcggg aatgctctcg gccacgagct gctcgtcatc
     2341 gtcaatcgct agtcgaagca cggactcgcg gtcggccgcc agcttgcccc gttgcccctt
     2401 cttcttgtgg tgcttcttgc ggggcttgag ggagaaggac gacgtagcaa acaggtaggt
     2461 gcggccggcc aaggtggccg actcgaggcc ttccatatca tcgatcgtgc ccggctctat
     2521 tcctctaata gggcgccgaa ccagggggca ggccatgctc ccgtccgggg ccagccgaag
     2581 ctcgaagaag gcatcgttca cgttgttgtc gcagaaaagg aaccgggagt cccccagggg
     2641 gaccacctcg gaggcattgt agggttcctt gcccggccgc tccccgaagg ggatcggccg
     2701 aagcttcctc ggcttggata cttcctcggt cacgacgtct tcttgagcgg cgtcgtgtag
     2761 ttgttcagct gaatctgccg ctggatgttc cggatccgtt ccgtcgggcg cgagtggtcg
     2821 ccggtaaaga agttggtcag cttgtcttcc tcgccgtatt tttgcttgaa tttttcccat
     2881 agccccggtc ctttggttgg gtcgaagccc gcctcataga tgtaacgcaa gcccacccgg
     2941 tcggcctgat cctcgaactc ccgactataa cccgacatga gcgcggagag gccgagctgt
     3001 ccgccgaagg ccgcgacctg ctgtgcggta gggttggaga tctgggtcag ggcaatggac
     3061 gcgcccacgc ccagaatctg ggccagtgtg cctttgctct gctggcgccg cacgtgctcg
     3121 tgggtgtagt gggtcagctc gtgacccagc acgatcgcca tctcctcgtc gctcattgcg
     3181 tccatcagac cggtatacac ccagatcgcg ccgttcgcca tggcagaggc gttccactgg
     3241 tcggtcttga cggcgcgaac tcggagcgcg ctggcgggaa cgtaggaggg ccgcagcttg
     3301 tcgatgatct tccgggcccg atcgacgtac gggcctgact ccagaatctc gccgatcttg
     3361 atgatcttcc cgctgtcggc gggctcgaac atcattttgt gcttgaccca ggtatcctcg
     3421 gcctggttgc tggccgcgat gatctcctgc tcggtgccgt cggtcccatt gggcctggta
     3481 tcgacctgtg ctgcgatcag cgcacccgac ttgtcccggc ttcccttgac cttgacctca
     3541 tagccgaggg ggatgtcctt aagcgtcttg gcgccgctca cccgggtcgc cggagttgcc
     3601 acgacccgct gaccatccac catcaccgtg tcaccccgct tgaactcggc ataaccggag
     3661 atcttctcgc tctggtcagc actgtggacc ggcggcctgc cgaccgggct caatgtcgcc
     3721 agcccggcgg cgagacaagc agccactgcc tggcgcacgt tggtcactgc tagagctcct
     3781 tcctttactt cacctgctcg acgtcaccta agctgccacc ggaacgacga gtcggcaaac
     3841 cgaccaaagg cagagatatg ccatctcagt cacgcgacag ggcccgtgac tgtcaatgct
     3901 gacggtcgac gggccccatc gcttggtgca ttggagctca agccccctga aggcctggag
     3961 gtcatctggt ccttcgtatc gccggtggcg tccacttttc gtcgaggatc gcgggcttgg
     4021 gccagtacga gcgtatgtag agcgagaagt cggcgtgctt gggggccggc agccagttcg
     4081 cctccttgtc cgtgccggga gattcattct ggatgtacag ggggagcgat ccatccgttc
     4141 cgagcttcag cgacttggcg ttcttggtgc caagcgaata ccgcttgagc ggattgggcg
     4201 agaagagatg gtcctcgttg tagagcgtga gcgaccagaa gccgttcacg ggaggcgtct
     4261 ctccctttgg gaacgtcgct gtgtaggcgt gagctccatt gaggcgctcg ccgcccgagt
     4321 cgaagtcggt atagatgtag cgcgtctctt ccggtgcgtt gtcgtacatg ttggatctcg
     4381 cagtagcggc acgggagaga tagtcgacgc cccagcgcgc gccgttaggc ggtgatgtcc
     4441 agccgtttcc gatcggccgc ccgttgttgt ggaactgaaa gaggggtccg atcaggtccc
     4501 gctccgcggc gatcgcggtc tcggtcaggg tctgcgtgag cttggggtcg ttcgccgcgg
     4561 cgtccagcac cgactggaac agggtgtaga gcgactcttc gcccggcagc ggcggcactt
     4621 tcttcattac gtccggtagc tgatcgaaga acttctccgg gatgacccac ttagtctcgc
     4681 cctttccgcc ggtcgccatc gggaaggacg gcactttggt ccagtccttg gttttcatct
     4741 tgccgtcgaa ctcggacagc ggatagacca tgatctggcg cagcaacggc tggatagcgg
     4801 ctctgtcctc gggcgtgtcg tcctggaaaa cacgcgggaa aaccgccgcc aggtccgtcg
     4861 acgagcggta gaccgcggtc acgcccgcgg gtacctgctc gttccagttc ggtccgacca
     4921 gcagatagaa tcccggtttg gtaccgtgct gctttcccac ggcgcagaac gcatccgttc
     4981 gcgcatcaca gatttggtag acccagaagc ggtcaccgaa gtccggcacc tgcacgatca
     5041 ccggcctgga gtcgagccgt tgaaacccgg caccgtagac ggtgtcctga ttcgggcagg
     5101 taacaaaccg ctcagactcc tcgatgtagt cggtgagcat cccgacgtag ccgggcggcg
     5161 ctgcggggag gataccgccg atatgcccgg gctcggggag ctgctcgaac gcggcggcgc
     5221 ggttgtggcc gttgaccagc ggccaggccc agatgtacgc caggcgaccg accgtctcga
     5281 catacgcgga ggtcatgacg gtgcgcggcg gtgttccggc aatctccttg gccggcttcg
     5341 taaccgcggc gctcctccga tctcgtactc gccgttcctc gcggatagct gacatgtgcg
     5401 ccccctgggg cctcttcgta gtttctgttt ctcgatttag ccacgaactt cggccggcgc
     5461 gatgaatgtt tgagccggct tggtttctac tccgccggcg tggcttcggg aaaacactcg
     5521 gcggtcaccg ggacagcctt ggcgcccgct ggggctgctg ttgcggtcat cactgtctcc
     5581 ttccaaatct ttctggcagc catcgtctgt gctcacttcc gaagcctgtt tttgaagagg
     5641 gttccgcggc cgtcatgctg tagcgccgcg ttgagctctt cctcggga
//
LOCUS       DASRJQ010000202         4609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1408252, whole genome shotgun sequence.
ACCESSION   DASRJQ010000202 DASRJQ010000000
VERSION     DASRJQ010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408252"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VFB89_04590"
     CDS             complement(<1..700)
                     /locus_tag="VFB89_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZM26608.1"
                     /translation="MSQSRIRNFCIVAHIDHGKSTLADRLIEATNTVEKRLMREQLLD
                     TMDLERERGITIKLNAVRMTYQGQDGTEYELNLIDTPGHVDFTYEVSRSLAACEGAIL
                     VVDASQGIQAQTLSNLFLAMDAGLEIIPVLNKIDLPGAEPERRAKEIMDLIGSKREEI
                     LAVSAKEGTGIPELLEAIVHRVPAPHGLEDAPLRALIFDSYYDKYRGAIPSIRVVDGV
                     LREGMEIAFGSHPDD"
     gene            895..1737
                     /locus_tag="VFB89_04595"
     CDS             895..1737
                     /locus_tag="VFB89_04595"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZM26609.1"
                     /translation="MLLVCLIWGTNFSVMKGAFSYLSPFAFSAVRFTISSLLLYGLLR
                     WRLGRVSVPPGLGWRMIWMGVLGNTLYQIAFTLSLVWGTATNSALILSTIPAMVTTLA
                     GLRRIEPVTTRMWVGTAVATVGVGLVILAGGVDFGAGTLRGDACAVAATLCWALYTIE
                     LRRLPPSMPPLQTTALTTFTGTPGLVAAGAMELVAIDWRRVPGPAWGALAYSSLLSLI
                     VAYMIWNASVQKVGSSRTAIYMCVTPLVAAVVAWLTLGERLVPLQGAGAVLIVTGVLM
                     TRKA"
     gene            1821..2165
                     /locus_tag="VFB89_04600"
     CDS             1821..2165
                     /locus_tag="VFB89_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZM26610.1"
                     /translation="MSESLPEFEAFRSRMNAAILGAGNLTINRFFALDNRAYEPGALG
                     TKTKEMLGLVSSLVLRCDDCITYHVVRCAEEGVTDAEFQEVFAVGLVVGGSIVIPHLR
                     RAVARLEEVRKA"
     gene            complement(2178..3692)
                     /locus_tag="VFB89_04605"
     CDS             complement(2178..3692)
                     /locus_tag="VFB89_04605"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HZM26611.1"
                     /translation="MTKAAALLLWFTLFLPFAPSPAFCQAPRPASAAAETITDHDVIR
                     HIKVIADDSMLGRDTPSHGLEATAQYVASQFKAAGLKPAGDSGTWFQRYPITRRHIDA
                     QASSITLRSGKTEARASLAAAARFVQGTVPESPVSGNVLLLGGVVTADAATGAGAAGR
                     IVLLVSDPQKPELEQAARALYLAGAKAIILLADVDSAAYAPRLSAQRRDRTAIDLGLA
                     RPTVIEVREGAVRRALAAAGLDPVALRATTKPVIRNLPKLTATITLKEVITSRHSAPN
                     TVGMIEGRDPKLKQEYVLFSAHMDHIGITPGQPDSINNGADDDASGTAAVIELASAFG
                     RPDARPKRSLLFLTVSGEEKGLWGSRYFSEHPPVPINQIVADINIDMVGRNWPDTIVA
                     IGKEHSDLGATLNRVNAEHPELGMTAIDDRWPEERFYFRSDHYNFARKGVPILFFFNG
                     VHPDYHRPSDSPEKINPEKEARIARLLFYLGEDIANAPERPKWKPESYKEIVEQ"
     gene            complement(3760..>4609)
                     /locus_tag="VFB89_04610"
     CDS             complement(3760..>4609)
                     /locus_tag="VFB89_04610"
                     /inference="COORDINATES: protein motif:HMM:NF025684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HZM26612.1"
                     /translation="RESPYRRGDGVRVYASTSVDGYVTVFRIDTDGGLRVLFPREPWA
                     DTWIRGGRLVRFDDAPGRSAFLVDDYPGIGYLFVITSPEPFDYSSIVRQDQWDYRGIA
                     NGRLHSDPYAALSQLADRLAPRGDYDYDVTPYYVERQYDYPRFVCYDCHSYSSYSNWN
                     PYGAACTRYRVVVYDDPIYYPYQAYGGRVVVPERPARLEPRYVFQDADGRSPWVSRTH
                     RSQSGSTEPVAPYRERQAESQPIPVRARQAPAQDRKISPTATQTTGEPELRRRRPDDK
                     TQGPDH"
BASE COUNT          783 a         1460 c         1475 g          891 t
ORIGIN      
        1 cgtcgtccgg atgtgaaccg aacgcgatct ccatcccttc acgcagcacg ccgtccacca
       61 cccgaatgct ggggatcgcc ccgcggtact tgtcgtagta ggagtcgaag atgagcgcac
      121 gaagcggggc gtcctccagg ccgtgggggg caggcacccg atgcacgatc gcctccagca
      181 gttccgggat cccggtgcct tccttggccg acaccgcaag aatctcctcc cgcttcgagc
      241 cgatcagatc catgatctcc ttcgcgcgac gctcgggctc cgccccaggc agatcgatct
      301 tgttcagaac cgggatgatc tcgagccctg cgtccatggc caggaaaagg ttggagagcg
      361 tctgtgcctg aattccctgg gacgcgtcca ccacgaggat cgccccttca cacgcagcca
      421 gcgaccggga gacctcatag gtgaaatcca cgtgccccgg cgtgtcgatc aggttgagct
      481 cgtattcggt gccatcctgc ccctgatagg tcatccgcac ggcgttcagc ttgatcgtga
      541 tgccgcgctc gcgctcgagg tccatcgtgt cgagcagctg ctcccgcatc aggcgcttct
      601 cgaccgtgtt ggtggcctcg atgagacggt ccgccagggt cgatttgccg tgatcgatgt
      661 gggcgacgat gcagaagttg cgaatgcggg actgggacat ggcgggataa tagtcgagag
      721 cgggcgggag cggacgggca ggcgcgaaca ggcggcagcg gaaccgagca tgtgttgtgt
      781 tactcttatg ccgtgacgac ccgcgagccc gcctccgccc gttcccgtcc gcttccgcct
      841 gcttccgcgg gccctccttc acccactcct cgcgcctctg ccgccgatct cggcatgctg
      901 ctcgtatgcc tgatctgggg cacaaacttc agcgtgatga agggcgcctt cagttacctg
      961 tcgcccttcg ccttcagcgc tgtgcggttc accatctcga gcctgctgct ctacgggctg
     1021 ctgcgctggc gattgggtcg cgtcagcgtt ccgcccggcc taggctggcg aatgatctgg
     1081 atgggcgtgc tgggcaacac cctctaccag atcgcgttta cgctgtcgct ggtctggggc
     1141 acggctacca actcggcgct gatcctgtcc actatcccgg cgatggtgac tacgctcgcc
     1201 ggcctccgtc gcatcgagcc cgtaaccacg cgcatgtggg tcgggactgc agtggcgacg
     1261 gtcggcgtgg ggctcgtcat cctcgctggc ggggtggact tcggcgcggg gacactgcgg
     1321 ggcgatgcct gcgccgtcgc agcgacgctc tgctgggcgc tctatacgat cgagctccgg
     1381 cggctccccc cctcgatgcc cccgcttcag accaccgcgc tcaccacgtt caccggaaca
     1441 cccgggctcg tcgccgcggg agcgatggaa ctggtggcga tcgactggcg gcgggtgcca
     1501 gggcccgcgt ggggggccct ggcctattcc tcactgctct ccctgatcgt cgcctatatg
     1561 atctggaacg cgagtgttca gaaggtcggc agcagccgga ccgcgattta catgtgcgtg
     1621 acgcccctcg tcgccgcggt cgtcgcctgg ctcacgctgg gggaacggct ggttccgctg
     1681 cagggcgcgg gagcggtgct gattgtaacc ggagtgctca tgaccagaaa agcgtgaacg
     1741 aaacagtgaa tggtgaacag gggccccgcg agcggtgagg ccccgttcac cgtttagcgt
     1801 ttaccgttta cgtttttcca atgagcgaat ccctcccgga gttcgaggcg ttccgctccc
     1861 gaatgaacgc tgccattctc ggggccggaa acctgaccat caatcggttc ttcgcgctcg
     1921 ataaccgcgc ctatgagccg ggcgcccttg gcaccaagac caaggagatg ctcggcttgg
     1981 tctcctcctt agtgctccgc tgtgacgact gtatcaccta ccatgtcgtg cgctgcgcgg
     2041 aggaaggggt caccgatgcc gagtttcagg aggtcttcgc agtggggctg gtggtgggcg
     2101 gaagcatcgt gatccctcac ttgcgcagag ctgttgcgag gctggaggaa gtcagaaaag
     2161 cttgaccatt cacgcagtta ttgctccaca atctccttgt agctctccgg cttccacttc
     2221 ggtcgttccg gcgcgttcgc gatatcctca cccagataga aaagcagtcg cgcgatccga
     2281 gcttctttct ccgggttgat cttctccggc gaatccgacg gacggtggta gtcgggatgc
     2341 acgccgttga agaagaagag gatggggacg cccttgcggg cgaagttgta atgatccgag
     2401 cggaagtaga agcgttcctc cggccagcga tcgtcgatgg ccgtcatgcc caactcgggg
     2461 tgctcggcat ttacccggtt caaggtcgcg ccgagatcgg aatgctcctt cccgatcgcg
     2521 acgatggtgt cgggccagtt gcggccgacc atgtcgatgt tgatgtccgc cacgatctgg
     2581 ttgatcggca ccggcgggtg ctcggagaaa tagcggctcc cccagagccc cttctcctcg
     2641 ccgctaactg tgagaaacag cagcgaccgc ttgggccgcg catcgggacg tccaaaggcg
     2701 gacgccagct cgatgactgc cgccgtgccg gaagcatcgt cgtccgcgcc attgttgata
     2761 ctgtcgggct gccccggcgt aatgccgatg tgatccatgt gggcagagaa cagcacgtac
     2821 tcctgcttga gcttgggatc cctcccctcg atcatgccta cggtgttcgg tgcgctgtgg
     2881 cgtgaggtga tcacttcctt gagagtgata gttgcagtga gcttcggaag gttgcgaatc
     2941 accggcttgg tcgttgccct gagggcgacg ggatccaggc ccgccgctgc cagggcccgg
     3001 cgcacagcgc cctcccgcac ttcgatcact gttggtcgtg ccaggccgag atcgatagcg
     3061 gttcgatcgc gccgctgtgc actgagccgc ggggcgtacg ccgccgagtc gacgtcggcg
     3121 agcagaatga tggccttggc accggccaga tagagcgcgc gggctgcctg ttccagctcg
     3181 ggcttttgcg gatcgctgac cagcagcacg attcgtcccg cggcaccagc tccggttgcc
     3241 gcgtccgcag tcaccacccc acccaacagc aggacgttcc cactgaccgg actttccggc
     3301 accgtcccct gcacaaaccg cgcggcagcc gccagtgatg ctcgtgcttc ggtcttgccg
     3361 ctcctgagcg taatactcga cgcctgggca tcgatgtgcc tgcgagtaat cggatagcgc
     3421 tggaaccagg ttccgctgtc ccccgcaggt ttgagtccgg cggccttgaa ctggctcgcc
     3481 acgtattgcg ctgtcgcctc gagcccatga ctcggcgtat cgcggccgag catcgagtcg
     3541 tccgcgatca ccttgatgtg cctgatcacg tcgtgatccg taattgtctc agcggccgcc
     3601 gacgccggcc ggggggcctg acagaatgcg ggagaagggg caaaagggag aaagagggtg
     3661 aaccagagga gcaaagccgc agcctttgtc atgtccgttc cgtgatcggg cgataggatc
     3721 gctacggcgc aagtcagccg gctgtttcaa ctggtcagct tagtgatccg gaccctgcgt
     3781 cttgtcatcg ggccttcggc gacgaagttc cggctcgccg gtagtctgcg tggccgtagg
     3841 cgaaatcttc cggtcctgtg ctggagcttg acgagcgcgt acgggaatcg gctgactctc
     3901 cgcctgccgc tcccgatacg gcgcgaccgg ctccgtggac ccgctctgcg atcggtgcgt
     3961 ccggctgacc cagggagacc ggccatcggc atcctgaaag acgtagcggg gctcgagccg
     4021 ggcggggcgt tccggcacca ccacacgacc gccgtacgcc tggtagggat agtaaatcgg
     4081 atcgtcgtaa acgacgaccc gatagcgggt gcaggctgcg ccgtagggat tccagttgga
     4141 gtacgaagaa tagctgtggc agtcgtagca gacgaacctg ggatagtcgt attgccgctc
     4201 gacgtagtac ggcgtgacgt cgtagtcgta gtcgcctcgt ggagcgagcc ggtcggccaa
     4261 ttggctgagc gcggcatagg ggtcgctgtg aagccggcca ttcgcgatac cgcgataatc
     4321 ccattgatcc tggcgaacga tgctggagta gtcgaacggc tccggcgagg taatcacgaa
     4381 gaggtagccg atgccaggat agtcatccac taaaaacgca cttcgacccg gtgcgtcatc
     4441 gaatcggacc aggcgcccgc cacggatcca ggtgtcagcc cagggctcac gaggaaagag
     4501 cacccgcaac ccaccgtccg tgtcgatccg aaacacagtc acatagccgt caacgctggt
     4561 actggcgtac acccgcacgc cgtccccgcg acgataggga ctttctcga
//
LOCUS       DASRJQ010000203         2055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1275484, whole genome shotgun sequence.
ACCESSION   DASRJQ010000203 DASRJQ010000000
VERSION     DASRJQ010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275484"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(546..752)
                     /locus_tag="VFB89_04615"
     CDS             complement(546..752)
                     /locus_tag="VFB89_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26613.1"
                     /translation="MADRYTPPRCQTMHVPSGARCQYFEGHLQRHFARVGEGPLGQKP
                     VEFGTARSAEDVERERKQKGAGDA"
     gene            complement(745..1011)
                     /locus_tag="VFB89_04620"
     CDS             complement(745..1011)
                     /locus_tag="VFB89_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26614.1"
                     /translation="MADRRRQKLDMAKAGQRSRAAGGRLLTHSIARNPRLVPLVNERT
                     AAAHARAFDAVIAGAPRKPPGPQLVQICRCADCLAKVEEAKSNG"
     gene            complement(1201..>2055)
                     /locus_tag="VFB89_04625"
     CDS             complement(1201..>2055)
                     /locus_tag="VFB89_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HZM26615.1"
                     /translation="ATASLRDVTTQDTYALYCSAHWTPHFKTIDGVTDAAIEEYTSAR
                     LGKVKSKTVNKELGALRALMVWAEGRGLCEPVTIRGVSKRTPGVAHPKGKRTHTELSP
                     AEVQKLLKALPVRTDERIIRGEKKLGGWPARAFFTVLYETTLRPETLWSIKVPGNYRK
                     GSKSLIIDNEQDKARFGRRVPLSAKARKALDEVCPAVGQIFPRYTYRKVAKAAARAAL
                     SPEKAATFHPYDLRAAGGTHRLERGAPIPGVQWLFGHRSVATTARYIRASERSAEEAV
                     SGRRKRSP"
BASE COUNT          303 a          702 c          691 g          359 t
ORIGIN      
        1 gagcctgctg atcgtcgcga ggtcgcattg ctcgttcgct cccctgggcg agtcattgcg
       61 ggtgtcgggg gacacgccac attcgccgct gtggcggttc cacatgccag gggtggcgcg
      121 ttggcggcgg cagtcggggc agtgcttagc catcggccct ccgttttgcg cgacaggtcc
      181 cgcagtaggc gaacgatgat cggccctcgc atccaaggca cttgccggcg gcccgcaggc
      241 gggatcgccg agcctttgcc caagctgccc gcgtggcgcg ctttcggtcg ctgaaattgt
      301 aaccgtgctt ggcgcagcgg attgagcagt ggaccgccaa ggggccgcgc acgccaagtc
      361 gatggatctg tctgcgacac acgaggcaca gccggtgccc tagcagcacg gcccgagccg
      421 ttgcggtcac gaacagcggg aaccgtctgc gaaccctttg aggctggccc gagtgcgcgg
      481 cttcgacgaa cacctcgagc acctcgagtt ggcgagcctc cacggactgc aagcgggcga
      541 acggctcagg catcgccggc ccccttctgc ttccgctccc gctcgacgtc ctcggcggag
      601 cgggcggtgc cgaactcgac gggcttctgc ccgagcggac cctcacccac gcgagcgaag
      661 tgcctctgaa gatggccctc gaaatattgg cacctcgcgc cgctcgggac gtgcatcgtc
      721 tggcagcggg gaggggtgta gcggtcagcc attcgacttc gcctcctcaa cctttgccaa
      781 gcagtcggcg cagcggcaga tttggacgag ttgcgggccc ggcggcttcc gcggcgcccc
      841 cgcgatcacg gcgtcgaagg cgcgggcgtg cgcggccgcc gtgcgctcgt tcaccagagg
      901 gacgagccgc gggttcctgg cgatgctgtg ggtcagcagg cgcccgccgg cagcacgaga
      961 tcgctggccg gctttggcca tgtcgagctt ctggcggcgt cggtcagcca aggcggcctc
     1021 ccgtccgaat cgttttaggg cggcacgccc tgtaacccct gccccgaggc gccttagagg
     1081 cccctgccct gcacgaaata gccgagaaat cgcgaccagc cgagagctct agctgcgttg
     1141 tggctccagt tgtcgcgggt tcgatccccg ttatccaccc caggaaatcg cgatctgatt
     1201 ctagggcgac cgcttccgac gccctgaaac tgcctcctcg gcggaccgtt ccgaagccct
     1261 gatgtagcgg gcggtcgtgg ccacggagcg gtgtccgaag agccactgga ccccggggat
     1321 cggcgcgccc cgctcgagcc gatgggtccc gccggcggcc ctgagatcgt acgggtggaa
     1381 cgtcgctgcc ttctcgggcg agagcgccgc gcgcgccgcg gccttggcga ccttccgata
     1441 ggtgtagcgc gggaagatct gaccgactgc cggacagacc tcgtccagcg ccttgcgggc
     1501 cttcgccgag agcggcaccc gccgaccgaa gcgggccttg tcctgctcgt tgtcgatgat
     1561 cagcgacttc gaacccttcc ggtagttgcc cgggaccttg atcgaccaga gagtctcggg
     1621 ccgaagagtc gtctcgtaga gcacggtaaa gaacgcgcgc gctggccagc caccgagctt
     1681 cttctccccg cggatgatgc gctcgtcggt cctcacgggc agcgccttga gcagcttctg
     1741 aacctcggcc ggcgaaagct ctgtgtgggt tcgcttgccc ttcggatgcg cgactccggg
     1801 cgtccgcttc gacacgccgc gaatcgtcac cggctcgcaa agtcccctcc cctcggccca
     1861 gaccatgagc gcccgaagcg cgccgagctc cttgttcacg gtcttggact tgaccttgcc
     1921 gagacgcgcg ctcgtgtact cctcgatcgc ggcgtcggtc acgccgtcga tcgtcttgaa
     1981 gtgcggcgtc cagtgtgccg agcagtagag agcatacgtg tcctgcgtgg tcacgtcccg
     2041 gagtgatgcg gttgc
//
LOCUS       DASRJQ010000204         3723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_998401, whole genome shotgun sequence.
ACCESSION   DASRJQ010000204 DASRJQ010000000
VERSION     DASRJQ010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998401"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..1564
                     /locus_tag="VFB89_04630"
     CDS             17..1564
                     /locus_tag="VFB89_04630"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZM26616.1"
                     /translation="MIRRLFWALALAAAVAACNPEPDRGATVLYASGADLQSINPLLT
                     SHPLARQVQRYMLLTTLARYDSTLEARPYLARTWSWSPDRRRLTFVLVTTVHWHDGVP
                     TTASDVRWTLQAARDPRVGYPRQTELADLESVTTPDDSTVVLDFAAAQARFPDVLTDL
                     AVLPAHLLDSVPLDALRRATWNQQPVGNGPFRFVRHDPNRRWVFAANPEFPAELGGPP
                     RLDRIVLAVVDEPVTKLAALVAGELDFAGIQPAHVAYVRRDSELNVLDYPLFFTYGLV
                     FNTRHPPFDRLDRRLAVSAALNRRELVDGYVYGFGTPATGPLVAGTEQSAQKQAGTGS
                     SRREPAGAEGSRRGPPDAEQTSKGTAQRISRPFPAPSSRLHPDDSSSQPIPFELLSVG
                     SGDGVLEQMIQAQLAQAGFRVTLRQLELSAFLDRLYGPSHDFDAAVLGIPGDVGLGYL
                     RPLAALTGLTPPQDPAAVARLFADSMPVAFLYHARGVQGMNRRVHGVRMDLRGELVTV
                     HDWWVSQ"
     gene            1561..2559
                     /locus_tag="VFB89_04635"
     CDS             1561..2559
                     /locus_tag="VFB89_04635"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26617.1"
                     /translation="MIRRARATAPVRLDFAGGWTDVPPFSAREGGVVVAAAIKLRAEA
                     EVVPGGDCLRLVADDLNQVIELAHPDELARDSGLPLLRAALRLNPPSGPLTLRTHSEA
                     PAGSGLGSSGAIDVALVAVLAEANGASPTRREVADTACRLEAHEAKIPGGRQDQFAAA
                     FGGFNLFRFHDPAVDIATLRLSDAFAAEFARRTILCYTGASHFSGGTIARVVQGYERG
                     EPAIVDALQRLRHVAEEMDQAIRAEDLARIGSLMAENWRRQQALDAGMCTPVMAALEA
                     ALRDAGALGGKAAGSGAGGCMFFLAGNDIAAARRAAVEAGARLLPVEWSWEGVRTW"
     gene            2535..>3723
                     /locus_tag="VFB89_04640"
     CDS             2535..>3723
                     /locus_tag="VFB89_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26618.1"
                     /translation="MGGRAHVVTAESLAGRKEVVAASSDLSALLARQVERARPVLERE
                     PVIPSVKALLSMDGGVCPDDGSPLRFDPWSPNAHTCPRCGRAQRGGRHDRWWARFQHL
                     WLGERAAQLAAIAVLGGESQAAAGARRILTHYGEHYFEYPNRDNVLGPSRLFFSTYLE
                     SIWLTNYLAAAVLLRENDQLDASTATAVGSLGEEAANLIGEFDEGLSNRQTWHSAALA
                     ALAVWFEDEDLLRRSVESSSGLLAHLLHGFGSDGLWVEGENYHLFALRGLLVGLDWIR
                     SVGVDPLEERPVAQRLEAALRAPALTALPDRTFPARKDSRFGVSLAQPMYLELWERGG
                     QLVRSSLDDRGEDILDWLAGLYSVAAPPAETFDSYLHEAGLPAPAHRSRADLSWWMLF
                     SMAP"
BASE COUNT          564 a         1220 c         1235 g          704 t
ORIGIN      
        1 gctgtagaat gacccggtga tccgacgact cttctgggcg ctggccttgg cggccgcagt
       61 cgccgcctgt aatccggagc ccgatcgcgg ggcgacggtg ctatacgctt ccggcgccga
      121 tctgcaaagc atcaatcccc tgctgaccag tcatccactc gcccggcagg tgcagcgata
      181 catgctgctg accaccctgg cgcgatacga cagcacgctc gaggccaggc cttacctcgc
      241 ccgaacctgg agctggtcgc ccgaccgccg aaggctgacc ttcgtgctcg tgactactgt
      301 ccactggcac gatggcgtac ccacgaccgc gagcgatgtg cggtggactc tgcaggcggc
      361 ccgcgatccc cgagtcggct atccgagaca gacagagctg gccgacctcg agtctgtgac
      421 gacaccggac gactcgacgg tggttctgga tttcgcggcg gcccaggctc gtttccccga
      481 cgtgctcacc gatctcgccg tgctgccggc ccatctgctc gattccgttc cgctcgacgc
      541 cctgcgccgc gcgacgtgga accagcagcc agtcgggaat ggaccattcc ggttcgtgcg
      601 gcacgacccc aaccggcggt gggtctttgc agccaacccg gagtttccag cagagctggg
      661 tggtcccccc agactggatc gaatcgtgct ggccgtggtg gatgagcccg tcaccaaact
      721 ggcggcgctc gtcgcgggag agctcgactt tgccggaatt cagcctgccc acgtcgcgta
      781 tgtgcgtcgc gactccgaac tgaacgtcct cgattacccg cttttcttca cctacgggct
      841 tgtgttcaat actcggcatc cgccgttcga ccggctggac cgtcgcttgg cggtctcggc
      901 cgcactgaat cggcgtgagt tggtagacgg ttacgtgtac ggcttcggaa ctccggcgac
      961 cgggcctttg gtggcgggaa cggagcagag cgcacagaag caggcgggaa cgggcagcag
     1021 tcggcgagag ccggcaggcg cggaagggag caggcggggg cctcccgatg ctgaacagac
     1081 gtccaagggc acggctcaga gaatttcccg gccgttcccg gcccccagct cgcgcctgca
     1141 cccggacgat tcgtccagcc aacccattcc tttcgagctg ctctcagttg gaagcggcga
     1201 tggcgttctc gagcagatga ttcaggccca gctggcgcaa gccggattcc gtgtcaccct
     1261 tcggcagctc gagctgtccg cctttctcga tcgactctac ggcccaagcc acgatttcga
     1321 cgctgcggtt ctcgggattc cgggcgatgt gggcctggga tatcttcgcc cacttgcggc
     1381 cttgacgggc ttgacgccgc cacaggatcc agcagcggtt gcgcggctgt tcgccgattc
     1441 aatgcccgtc gccttcctct atcatgcacg aggcgttcag ggcatgaatc gacgagtgca
     1501 cggggtgcgc atggatcttc gcggcgagct ggtcacagtc cacgactggt gggtgtcgca
     1561 gtgatccggc gagcgcgcgc cacggctccg gtgcgcctcg actttgcggg tgggtggacc
     1621 gacgttccac ccttctcggc cagggaaggt ggcgtggtcg tggccgcggc aataaagctc
     1681 cgcgccgagg cggaagtcgt tccgggtgga gactgcctgc ggctcgttgc cgacgacctg
     1741 aatcaagtga tcgagctggc tcatcccgac gaactggcgc gggactccgg ccttccactg
     1801 ctgcgagcgg cgctgcggct caatccaccc tccggcccgc tgactctccg cacccactcc
     1861 gaagcacctg ctggctccgg cctcggcagc tccggagcga tcgatgttgc gttggtcgcc
     1921 gtgcttgctg aagcaaatgg tgcgtctccg actcggcgcg aagtcgccga caccgcctgt
     1981 cgcctcgagg cccacgaggc aaaaatcccc ggcggccggc aggatcagtt cgccgcggca
     2041 ttcggtggct tcaacctgtt tcgctttcac gatcctgccg tcgacatcgc gacgctgcgg
     2101 ttgtcggacg catttgctgc cgagtttgcc cgtcggacga tcctgtgcta cacgggcgcg
     2161 tcgcatttct cggggggaac catcgcgcgg gtggtgcagg gatacgagcg cggcgagccg
     2221 gcaatcgtgg atgccttgca gcggcttcgc catgtggctg aagagatgga ccaggcgatt
     2281 cgggctgaag atctggcgcg catcggatcg ctcatggcgg agaactggcg acgacagcaa
     2341 gccctcgatg ccggcatgtg cacgccggtc atggccgcgc tcgaggctgc gctccgcgac
     2401 gcaggggcgc tcggcggaaa ggcggcgggg tcgggagcgg gaggctgcat gttctttctt
     2461 gccggcaacg atatcgcggc ggctcgtcgt gcagccgtcg aggcgggtgc gcgccttctg
     2521 ccggtggaat ggtcgtggga gggcgtgcgc acgtggtgac cgccgagtcc cttgccggtc
     2581 gaaaagaagt cgtcgcggca tcgtccgacc tctcggctct tctcgcgcga caggtcgagc
     2641 gtgcgcgccc ggtcctcgag cgcgagcctg tgattccatc ggtcaaagcc ctgctttcca
     2701 tggatggcgg agtgtgtccg gacgatggca gccctcttcg cttcgatcca tggagcccca
     2761 atgcccatac ctgtccccgg tgtgggaggg cccaacgcgg cggtcggcac gatcggtggt
     2821 gggcccgctt ccagcatctc tggctcggcg aacgcgcggc tcagctcgct gcgattgccg
     2881 ttctcggcgg cgagagccag gccgcagccg gcgcgcgccg aattctcacc cactacggcg
     2941 agcattattt cgagtacccc aatcgggaca acgtactcgg tccgagtcgc ctgttctttt
     3001 cgacctacct cgagtcgatc tggcttacca actacctggc cgccgcagtg ctgttgcgtg
     3061 agaacgatca gctggacgcg tcgacggcga cggcggtcgg cagcctcggg gaggaagccg
     3121 ccaatctgat cggcgagttc gacgagggcc tctccaaccg acagacctgg cacagcgccg
     3181 cgctcgctgc cctggcggtc tggttcgaag atgaagacct gctgcggaga tcagtggaga
     3241 gctcgagcgg gcttctggct catcttctgc acggtttcgg cagcgacggt ctctgggtcg
     3301 agggcgagaa ctaccatctc tttgcattgc gaggtctcct cgttggtctc gactggattc
     3361 gctcggtcgg ggtagatcca ttggaggaac ggccggtcgc ccagcgcctg gaagctgcgc
     3421 ttcgggcacc tgccttgacc gcgcttcccg accgcacgtt ccccgcacgt aaggattcgc
     3481 gtttcggcgt gtcgctggcc cagccgatgt acctggagct gtgggaacga ggtggccagc
     3541 tggtgcgctc gtcgcttgac gatcgaggtg aggacatcct cgactggctc gctggcctgt
     3601 attccgtggc cgctccgccc gccgaaacgt tcgactcgta ccttcacgag gctggcttgc
     3661 ccgcgccggc gcatcggagc cgcgccgatc tctcgtggtg gatgctgttc agcatggcgc
     3721 ctc
//
LOCUS       DASRJQ010000205         3067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_712347, whole genome shotgun sequence.
ACCESSION   DASRJQ010000205 DASRJQ010000000
VERSION     DASRJQ010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712347"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VFB89_04645"
     CDS             <1..369
                     /locus_tag="VFB89_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26619.1"
                     /translation="RLILIASHNLDELERLADRVLILHQGRLQRVVTAGAGPAEAAVA
                     RPLVYRLALTTKHAAVGRVFPAAEAVEGRDAEWLVRGDLETLNRGLAELVGAGALVAS
                     FTPEQSRLESEFRAAVRGEP"
     gene            366..1079
                     /locus_tag="VFB89_04650"
     CDS             366..1079
                     /locus_tag="VFB89_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26620.1"
                     /translation="MSGLPTRYAGWMVRDFAAGPAILMVVLSGLALLVVRNLQVVGPS
                     GNADLAVCFVALDWTTMSLTLVATAGLVSGDFSQGHQRTLFAKPLNPSLYYLQRWLIG
                     GVAVGVATLPIAYAIASRFAVPVIGLGFFARLALLYLVLGGLVFLLSTITRRDWLVAI
                     VLIAWQAALGAAHSAGLAHGPLTEALIAILPPFQRIGLRGPTPEFAGLVHALGYGALL
                     LGAALAVLQWRPLARGARD"
     gene            1079..>3067
                     /locus_tag="VFB89_04655"
     CDS             1079..>3067
                     /locus_tag="VFB89_04655"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HZM26621.1"
                     /translation="MQWSAGVVREVLPNGLTLLVQPERSAPVVAVVTHVKAGFFDEPD
                     RWVGISHVLEHMFFKGTARRGVGAIARETKAAGGYLNASTSYDHTTYFTVLPAGGLEA
                     ALDIQSDALRNSAIDADELARELRVIIQEAKRKLDNPPALAYETLHEIMFDRHRIRRW
                     RIGHEAALAGFTREDVRGYYRSRYVPERTIVSIVGDVDPEKAITLAKARYADWPPARG
                     AEDPSPEEPIRREIRARTLRGDISQAELAVGWRTAPPLHPDAAPLDLAAAVLASGRGS
                     WLQRSLRETGLVISVSAHNYTPTELGVFSVTADLEADRALPALEGIASAVSRLALLGP
                     SEADLDRARTLLLTRWARRLESMEGRASALAAAEALESVDVLDREYAALSRATAADIR
                     TVAGRYLDPESVAALAYLPRNRGEDLTTDRVAGIFAVTPLTSGTLTIAPPPRAAAPQV
                     ARGQSQAGVLHVALPGADLLIRPKSGVPTVSLGLYVPRFEFEPAVQAGLGALVARAAL
                     RGAGDLDAGALAFAAERLGGSLSAVLNSDWLGFGLTVLSDRLPEAAALLDIVYRLPRL
                     SPEDIAGERDALTAEAKQLADDMFRFPFQLAFGAAFDDRGYGLPVAGLGETLASLTAE
                     DARRWHETAFLGRRPVIVVVGDLESGAAADALAGVFASL"
BASE COUNT          446 a         1025 c         1025 g          571 t
ORIGIN      
        1 cgcctgatcc tcatcgcctc gcacaacctg gatgagctcg agcgactcgc cgaccgggtc
       61 ctcatcctcc accagggaag gctgcaacgt gtagttacgg ccggtgcggg cccggccgag
      121 gcggcggtgg cccggccctt ggtgtaccgg ctcgccttga ctacaaagca tgcagcggtc
      181 ggccgcgtgt ttcccgccgc cgaggcggtc gaggggcgcg atgccgagtg gctggttcga
      241 ggcgatctcg agacgctgaa tcggggcctt gccgagctgg tgggggccgg ggcgttggtg
      301 gcgtcgttca caccggagca aagccggctg gaaagcgaat ttcgagcagc ggtccgaggt
      361 gaaccgtgag cggacttccg acgcgctatg ccggttggat ggttcgtgac ttcgccgccg
      421 ggccagccat tctaatggtt gtgctctccg ggctggcgct gctcgtcgtg cgcaaccttc
      481 aggtggtcgg ccccagcggt aacgcagatc tcgcggtatg ctttgtcgcg ctcgactgga
      541 ccaccatgag cctcactctc gtggcgaccg ctggattggt gagcggcgac ttttctcagg
      601 gacatcagcg gaccttgttc gccaagccgc tcaacccgtc cctctattac cttcagcgct
      661 ggctcatcgg tggcgttgcg gttggagtgg ccacgcttcc aatcgcttac gccatcgcaa
      721 gccgtttcgc tgttcccgta atcgggctgg gcttcttcgc ccgattggct ctgctctatc
      781 tcgtcctcgg cggtctcgtg tttctccttt cgaccatcac tcggcgagac tggctggtgg
      841 cgatcgtgtt gattgcctgg caggccgcac tgggggctgc ccactccgcc ggtcttgcgc
      901 acggcccgct caccgaagcg ctgatcgcaa ttctgcctcc ctttcagcgg atcggacttc
      961 gcggaccaac gccggaattc gcagggctgg tgcacgcctt aggatacggc gccctgcttc
     1021 tcggggctgc cctggcggtg ttgcaatggc gccccctggc ccgcggagct cgcgactgat
     1081 gcaatggtct gccggcgtgg tgcgcgaggt cctgccgaac gggcttacgc tgctggttca
     1141 gccggagcgc tctgcgccgg tggtcgccgt cgtcacccac gtcaaagccg gtttcttcga
     1201 cgagcccgat cgctgggtgg gcatttcgca cgtgctcgaa cacatgttct tcaagggcac
     1261 tgcgcggcga ggcgtggggg ccatcgctcg ggagaccaag gcggccggcg ggtacctgaa
     1321 cgcaagcacg agctacgacc acacgaccta cttcacggtg ctgccggcag gtggtctcga
     1381 ggcggctctc gacatccaga gcgatgcctt gcgcaattcg gccatcgatg ccgacgagct
     1441 tgctcgcgag ttgcgcgtga tcatccagga agccaagcgt aagctggaca acccgcctgc
     1501 gctggcgtac gaaacactgc acgagatcat gttcgaccgg caccggatcc gccgctggcg
     1561 catcggacac gaggcggctc tcgccgggtt cactcgggag gacgtccggg gctactaccg
     1621 gtcccggtac gtacccgagc ggaccattgt ctcgatcgtg ggagatgtcg acccggagaa
     1681 ggcgatcacg cttgcaaagg cacgctatgc cgactggccg cccgcgcgag gcgccgagga
     1741 tccttcaccg gaggagccaa tcaggcggga aatccgagcg cgcacgcttc gcggcgacat
     1801 atcgcaggcc gagctggcgg tcggctggcg aaccgccccg ccgctccatc ctgatgctgc
     1861 cccgctcgac ctcgctgctg ccgttctcgc gtccggccgg ggcagctggc tgcaacgatc
     1921 gttgcgtgag accgggctcg tcatcagcgt ctccgcccac aactacacgc cgacggagct
     1981 tggcgtcttc agcgtgaccg ccgacctcga ggccgacaga gctctgcccg ccctggaggg
     2041 gattgcgtcg gcggtctcgc gcctggcgct gctgggacca tccgaagcgg acctcgatcg
     2101 agcccgcact ctgctgctca cgcgctgggc ccgccgcctc gagtctatgg agggaagggc
     2161 gtccgcgttg gccgcagctg aggcgctcga atccgtggac gtactcgatc gggagtatgc
     2221 cgcgctgtcc agggccactg cggccgacat acgaacagtc gccgggcgct acctggatcc
     2281 cgagtccgtc gcggcattgg cgtacttgcc tcgcaatcga ggagaagatc tcacgaccga
     2341 tcgggtcgca gggatcttcg cggtaacgcc cctcacgagc ggaaccttaa cgatcgcgcc
     2401 gcccccccgg gcagctgccc cccaggtcgc ccgcggtcaa agccaggcgg gagttctgca
     2461 cgtcgcgcta cccggcgccg accttctgat ccggcccaag tcgggagtgc cgacggtcag
     2521 ccttggcctg tatgtgccca ggttcgagtt cgagccggcc gtccaggcgg gcctcggtgc
     2581 actggtggca cgcgccgcgc ttcgtggcgc aggagacctc gatgcgggtg cgctcgcctt
     2641 tgcagcggag cggttgggcg ggtccctcag cgctgttctc aactcggact ggctcggctt
     2701 cggcctcacg gttctctcgg accggttgcc ggaagcggcg gcactgctcg acatcgtcta
     2761 ccggctgcca cggctgtcgc ccgaagacat tgccggcgag cgggatgctt tgaccgccga
     2821 agcgaaacag ctcgcggacg acatgtttcg attccctttt cagctcgctt tcggtgcggc
     2881 attcgacgat cggggctacg gacttcccgt cgcgggcttg ggcgagacct tggcgtcgct
     2941 cacagcagag gacgcccggc gctggcacga gaccgcgttt ctcggccggc ggcctgtgat
     3001 cgtggtcgtg ggagacctcg aatcgggagc tgccgcagat gctctcgcgg gagtattcgc
     3061 ctccctc
//
LOCUS       DASRJQ010000206         4186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3813009, whole genome shotgun sequence.
ACCESSION   DASRJQ010000206 DASRJQ010000000
VERSION     DASRJQ010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3813009"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VFB89_04660"
     CDS             complement(<1..463)
                     /locus_tag="VFB89_04660"
                     /inference="COORDINATES: protein motif:HMM:NF024489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26622.1"
                     /translation="MTQVSPPPQLAAEPPVLDLRHPSLYLNRELSWLEFNRRVLHEAL
                     DQRTPLLERLKFLGIFSSNLDEFFQVRVAGLKQQLAAGIVERTADGMTPEDQLRAICS
                     TVREQFRLHQDCLMRDVIPALAEHGVHLVLQVTDLSPTDRAHLDRYFQANVF"
     gene            complement(571..939)
                     /locus_tag="VFB89_04665"
     CDS             complement(571..939)
                     /locus_tag="VFB89_04665"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HZM26623.1"
                     /translation="MRHLLIRLIINAAALFIAVQLIPGMHFTGGIGKLLLVALVFGLV
                     NAVVRPVLTMLSCPLIVVTLGLFLLVINAAMLGITSWLSTRLDLGFHVDGFVAALLGG
                     LVIGLVSTLLTLFVGRAAQG"
     gene            complement(949..2979)
                     /locus_tag="VFB89_04670"
     CDS             complement(949..2979)
                     /locus_tag="VFB89_04670"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZM26624.1"
                     /translation="MRIGGALALLVSAIGLNPNPVLPQTGARHPLGVDDFLALKLASD
                     PQLSPDGKLVAFVVSVPSLTENRNVGRIWLARTDRDSAWQITDGTGTDRAPRWSPDGQ
                     RLGFLSTRDGVTQVWEIPVRGGEPTKLTSVSSSVSDFQWSRDGRDLFLVSDLKWPSQS
                     EVERRNGEYPTDARIWTSLFYRHWNEWRVGLRQHVLRYSLATQTLTDLTPIDRDVPTL
                     ALGGHDLALAPSGREVAVTSNSDSSVATSTNNDIFVISLDGDGFKPITTNPANDHSPA
                     YSSNGRYIAYLAAQRPGFESDRQQLMLYEPANGRRRSLTADWSLSISAFTWLPDSRGV
                     VAEVEERGQGVLYRIDVPGGKRSRLIAGGRNSSVQVAGKGSLLAFLHQSATAPPEVWA
                     MNPDGKDQRALTALNRTALAQVDVRPLESFGFVGAGGDSVFGWLLKPPQFDSTKSYPL
                     LYLIHGGPQSAWADEWHQRWNYAMFAARGYVVAAVNPHGSTGYGQAFTDAVSRNWGDG
                     AYDDLMKGLDAVLGGRSYIDPKRLGAAGASYGGYMIYWIAGHTTRFRALAAHDGVFNP
                     VSMSGSTEELWFPIWEFGGSQLSPNARAIMEKWSPASSIANWSTPMLIVHGQQDFRVD
                     VSEGYQAFSALKLRGVPAKFLYFPDEGHFVLKPRNRRLWWGTILDWFDSFLR"
     gene            complement(2976..4034)
                     /gene="mnmA"
                     /locus_tag="VFB89_04675"
     CDS             complement(2976..4034)
                     /gene="mnmA"
                     /locus_tag="VFB89_04675"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HZM26625.1"
                     /translation="MAERVLVAMSGGVDSSVAAALLVESGYDVVGATMKLFCYGDSVP
                     DRPCCSLDSIEDARNVAYTLGIPHYVLNLEDRFNLHVIRNFVDEYSRGRTPIPCVRCN
                     SFTKFRDLLAHADALDCRYLATGHYVVARDGALFRGRDPRKDQSYFLWGIDRSVVARM
                     LTPVGSLTKSETRAYARRLGLVTADKKESVEICFVPDNDYVAVLERHLPAGAAALEPG
                     AFVTTSGDVIGEHAGFARFTVGQRKGLPGGASEARFVVAIRPETREVVVGTGRDLAGS
                     HVRLDEINWLADPLTPGDTCAVQLRYRAAAVGATVLASEDGGLELALAQPVRAISPGQ
                     SGVLYGLGERVLGGGVIA"
BASE COUNT          851 a         1335 c         1309 g          691 t
ORIGIN      
        1 gaaagacgtt cgcctggaag taacgatcga gatgcgcccg atcggtcggg ctcaggtcgg
       61 tcacctgcaa gaccagatgc acaccgtgtt cagccagagc cggaatcacg tcgcgcatga
      121 gacaatcctg atgcaaccgg aactgctcac gtacggtcga gcagatcgcc cggagctgat
      181 cctccggggt catgccgtcg gcagtccgct ctacgattcc ggcagcgagt tgctgcttga
      241 gaccggccac ccggacctgg aagaactcgt ccagattgct gctgaagatg ccgagaaact
      301 tgaggcgctc gagaagggga gtgcgctggt ccaacgcttc gtggaggacc cggcgattga
      361 actcgagcca ggacaactct cgattcagat agagcgacgg atgccgaaga tcgaggaccg
      421 gcggctcagc cgcgagctgg ggtggaggcg atacctgggt catgcctacc aatgtaccca
      481 ccgcggcccg cttgggggaa tcgcctgtaa cggcttcgta acagacggtg ggacaagggg
      541 ttatgccgat gtccccgatc aggccatccc tcagccctgt gccgcccggc ccacaaagag
      601 cgtgagcagg gtgctcacca ggccgatgac caggccgccc agcagggccg caacgaagcc
      661 gtctacatga aacccgagat cgagtctggt agagagccag gaggtgatac ccagcatcgc
      721 ggcgttgatg acgaggagga atagccccag cgtgactacg atgagaggac aactaagcat
      781 cgtaagcacg ggccgtacga cggcattcac taatccgaac accagcgcga ccagcaggag
      841 ctttcctatc ccgccggtga aatgcatccc cggaatgagc tgaaccgcga tgaagagcgc
      901 ggctgcattg atgatgagac gaatcaatag atggcgcaca gtagacgctc accgaaggaa
      961 gctgtcgaac cagtcgagga tcgtccccca ccagagcctg cgattccttg gcttgagaac
     1021 gaaatggccc tcgtcgggaa agtacaggaa cttggccgga acgccacgga gcttgagcgc
     1081 gctgaacgcc tggtagcctt ccgacacatc cactcgaaag tcctgctggc catgcacgat
     1141 cagcatggga gtggaccagt tggcgatcga gctcgcgggc gaccatttct ccatgatcgc
     1201 tcgcgcgttg ggcgagagct gcgagccccc gaattcccaa atgggaaacc agagctcctc
     1261 ggtcgagccg ctcatcgaga cgggattgaa cacaccgtca tgggcggcca gggccctgaa
     1321 gcgggtcgtg tgcccggcga tccaatagat catatagccg ccgtaggagg cgcctgcagc
     1381 tcccagtcgc ttgggatcga tgtaggatct gcctcctaag acggcgtcga ggcctttcat
     1441 cagatcgtca tatgcaccat cgccccaatt gcgcgagacc gcatcggtaa atgcctgtcc
     1501 gtatccggtg gacccgtgcg gattcaccgc cgcgacgacg tacccacggg ccgcgaacat
     1561 ggcgtagttc caacgctgat gccactcgtc ggcccaggcg ctctgcgggc cgccgtggat
     1621 cagatagagt aggggatagg acttggtcga atcgaactga ggtggcttga gtagccagcc
     1681 aaacacggaa tcgccaccgg cgcccacgaa tccgaacgat tcgagcggcc tcacgtccac
     1741 ctgggcgaga gcagttcggt tgagggcggt aagagcccgc tgatccttcc catccggatt
     1801 cattgcccag acctctggcg gcgcggtggc ggattggtga aggaaagcca gaaggcttcc
     1861 ctttccggcc acctgaaccg agctgttcct gcccccggcg atcagcctgc ttcgctttcc
     1921 ccccgggaca tcgatcctat acagaacgcc ttgcccgcgt tcttcgactt ccgccaccac
     1981 accgcgcgaa tcggggagcc aggtaaacgc cgaaatgctc agcgaccagt cggcggtcag
     2041 cgagcgccgc cggccattgg ccggctcgta gagcatgagc tgttgccgat cggactcgaa
     2101 ccccggtcgt tgggccgcga ggtacgcgat gtaccgacca ttggacgagt acgcggggct
     2161 gtggtcgttg gccggattcg tggtaatcgg cttgaatccg tccccgtcga gcgaaatgac
     2221 aaagatgtcg ttgttagtgc tggtggctac actcgagtcc gagttggatg tcaccgccac
     2281 ctctcgcccg gatggagcca gggcgagatc gtgcccgccg agcgccaggg tcggaacgtc
     2341 tcgatcgatc ggcgtcaggt ccgtaagggt ctgcgtcgcc agggaatatc gaagcacgtg
     2401 ctggcggaga ccgacacgcc actcgttcca gtggcgataa aaaagtgagg tccagatccg
     2461 ggcatcagtc ggatattcgc cgtttcgccg ctcaacttcg gactgcgacg gccacttgag
     2521 gtcggagacg agaaagagat cacgcccgtc gcgagaccac tggaaatcac tcacgctgct
     2581 cgacaccgat gtcagcttgg tgggctcacc cccgcggacg gggatttccc agacctgggt
     2641 gacgccgtca cgggtcgaga gaaagccgag gcgctgtcca tccggactcc accggggtgc
     2701 ccgatcggtt ccggtgccgt cggtgatttg ccacgcactg tctcgatcgg tgcgcgccag
     2761 ccagatcctg ccgacatttc gattttcagt gagtgacggg acggagacaa cgaatgcaac
     2821 cagctttcca tcaggggaaa gctgcggatc acttgcaagc ttgagtgcga ggaagtcgtc
     2881 gactccgagc ggatgccgcg cacccgtctg aggcaggacg ggattcgggt taagaccaat
     2941 ggcggagacc agcagggcaa gcgccccgcc gattctcatg cgatgacccc gccgcccaat
     3001 acccgctcgc cgagcccgta cagcacgccc gactgcccgg ggctgatcgc acgaaccggc
     3061 tgggccaaag ccagctcgag cccgccgtcc tccgacgcga gtacagtcgc cccaaccgcc
     3121 gccgcgcggt agcggagctg cacggcacag gtgtctccgg gcgtaagcgg gtcggccagc
     3181 caattgatct cgtccaagcg gacgtgcgaa cccgccagat cccggccggt acctaccacg
     3241 acctcgcgcg tttccgggcg aatcgctacg acgaagcgcg cctctgaggc acctcccggc
     3301 agtccctttc gctgcccgac ggtgaatcgc gcaaagcccg cgtgctcacc aattacgtcg
     3361 ccgctggtcg tgacaaatgc gcccggctcg agcgccgcag cgccggccgg caggtgccgc
     3421 tccagcacgg cgacgtagtc gttgtccgga acgaagcaga tctccactga ttctttcttg
     3481 tctgccgtga ccaggcccag ccggcgagca taggctcggg tttcgctctt ggtgagactg
     3541 cccacgggcg tgagcatccg ggcgaccacg ctgcggtcga tcccccagag gaagtagctc
     3601 tgatccttcc tgggatcccg cccccggaac aacgcgccgt cccgcgcaac gacgtagtgg
     3661 ccggtcgcga ggtaacgaca atcaagcgcg tcggcatgag ccaggagatc gcgaaacttg
     3721 gtgaacgaat tgcagcggac gcaaggaatc ggcgtccgtc cccgactgta ctcgtccacg
     3781 aaattccgga tcacgtggag gttgaaccga tcctccaggt tcagcacgta gtgcgggatt
     3841 cccagcgtgt acgccacgtt ccgcgcatcc tcgatggaat ccagcgagca gcaaggccgg
     3901 tccgggaccg agtcgccgta gcagaagagc ttcatcgtag cgccgaccac gtcgtacccg
     3961 gactcgacca gcagggcggc ggcaaccgag ctgtccacgc cgcccgacat ggcgaccagc
     4021 acgcgctcag ccatggctcg agattcctgc gaccgagcgc actcgtgcga ccacgcgcgg
     4081 gaactccccc acgactcgct cgatgtcggc ttcggtcgtg gtgcgtccca ggctgaagcg
     4141 aaccgcgcta atggcgaggt cccgaggaat acccatcgcg accagg
//
LOCUS       DASRJQ010000207         3323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2687303, whole genome shotgun sequence.
ACCESSION   DASRJQ010000207 DASRJQ010000000
VERSION     DASRJQ010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2687303"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2977
                     /gene="secA"
                     /locus_tag="VFB89_04680"
     CDS             <1..2977
                     /gene="secA"
                     /locus_tag="VFB89_04680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HZM26626.1"
                     /translation="ERLTDDQLRARTDEFRAQIDRAREEGRNAEHGEELVADLLHDLL
                     PPAFATVREGARRVLGQRHFDVQLMGGAALHLGWVAEMKTGEGKTLVATLPSYLNALA
                     GRGVHLITVNDYLASFHADWMGQLHRFLGLSVGKIIPDDSTAEQKRAQYDCDITYGTN
                     NEFGFDYLRDNMVTAIEDQVQRGQRFHSLSPHYYAIVDEVDSILIDEARTPLIISGPV
                     GNAEDDKYAQFNRQVAELVRKQTTVVNTLLAEAEKLLEGDGKGRDEAALKLYQAQLGM
                     PKNKRLLKVLNETGVKQMVQKMELETIADRKLPMKQQRMRDLEEALYFVLDERGHSVH
                     LTDQGSTALSPGEPTLFVVPDISEAVHALEKDPDLSPHDRIARRREIEAEYAFKSEKL
                     HIIHKLLQAHALYEKEVDYLVQEGQVLIVDEFTGRIMHGRRWSDGLHQAVEAKEGVEV
                     KGETQTFATITIQNYFRMYDKLAGMTGTAETEENEFYQIYGLEVSVIPTNKPVRRNDR
                     QDHIYKTRREKYNAILDEVERLHGLGWPILIGTVTVDVSETLSKMLKRRGLKHEVLNA
                     KYHQREAEIVAQAGKVNAITIATNMAGRGTDIKLDPALDLAKEEAGLQIIGTERHESR
                     RIDRQLRGRSGRQGDPGQSIFFLSLEDDLMRLIGSDRIARWMDKSGAEEGEVITGGLV
                     TSAIGKAQQRVELQNFQARKRLLEYDDVMNQQREVVYSLRLFALERGEELKAEAQRMI
                     EAALERATRQFLVDAERPEEYDRGGLRELLTMQYLVSVEAVLDPKQTPEIESLVAAAR
                     TEGDATFHRKIDYLQEFGRAIGIRDVDAQVLSQVMLSVLDEKWKDHLYDLDQLRNAIH
                     YRGWGQKDPLVEYKKEAYDMFEDLMHDIQSTFTERFLKVQVSADQPPPPRPQPPPLPP
                     TPESTGPDDLFMGAGPATKMPASVPAPSGAETGRSSTVTSSLGPVGRTATAVPPVGRN
                     DPCPCGSGKKYKKCHGAGQ"
     gene            2956..>3323
                     /locus_tag="VFB89_04685"
     CDS             2956..>3323
                     /locus_tag="VFB89_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26627.1"
                     /translation="MSRSWSVTSGAVALLSLVTYGLPARAQTEAAARAVSLDSEAELK
                     QLRRAQRDFERLRESNAPALRFDLPSRCDLSFGVYCYHFNTPDEETPPEPPETDAARS
                     ELLERLEDGARLLPGDPWIAG"
BASE COUNT          672 a         1066 c         1075 g          510 t
ORIGIN      
        1 cgaacggctc accgacgacc agctccgagc ccgtaccgac gagttccgcg cccagatcga
       61 ccgggcccgg gaggagggcc gcaacgccga gcacggcgaa gagctcgtgg ccgacctcct
      121 gcacgatctg ctcccgcccg ccttcgcgac cgtgcgcgag ggggcccgcc gggtgctcgg
      181 tcaacgccat ttcgacgtcc agctcatggg tggcgcggcg ctgcacctgg gttgggtggc
      241 cgagatgaag accggcgagg gcaagaccct cgtcgccacg ctcccgtcgt acctcaacgc
      301 actcgccggc cggggcgtgc acctcatcac cgtgaacgac tatctcgcct ccttccacgc
      361 cgactggatg ggacagcttc accggttcct cggcctctcg gtcggcaaga tcatccccga
      421 cgactcgacg gccgaacaga agcgtgcgca gtacgactgc gacatcacgt acggcacgaa
      481 caacgaattc ggattcgact acctgcgcga caacatggtc accgcgatcg aggatcaggt
      541 gcagcgcggc cagcgcttcc actccctctc gccgcactac tacgccatcg tcgacgaggt
      601 cgactcgatc ctgatcgacg aggcccgcac gccgctcatc atctcgggac cggtcggcaa
      661 cgccgaggat gacaagtacg cccagttcaa tcggcaagtg gccgagctgg tgcgcaagca
      721 gaccacggtg gtgaacacgc tgttggccga agcggagaag ctgctggagg gcgacggcaa
      781 gggccgcgat gaagcggcgc tcaagctcta tcaggcgcag ctgggcatgc ccaagaacaa
      841 acggctgctc aaggtcctga acgaaaccgg cgtcaagcag atggtccaga aaatggagct
      901 cgagaccatc gccgatcgca agctgcccat gaaacagcag cggatgcgtg atctcgagga
      961 agcgctctac ttcgtgctgg acgagcgagg ccattcggtg cacctgaccg accagggctc
     1021 gactgcgctt tcgccgggcg agccaaccct gttcgtggtg ccggacattt ccgaagccgt
     1081 ccatgcactg gagaaggacc ccgatctctc gccgcacgat cggatcgctc gccggcgcga
     1141 aatcgaagcc gagtacgcct tcaagagcga gaagctgcac atcatccaca agctgctcca
     1201 ggcccacgcg ctgtatgaga aggaagtcga ttatctcgtc caggagggcc aggtgctgat
     1261 cgtggacgag ttcaccggcc gcatcatgca tggccggcgg tggtcggacg gactgcacca
     1321 ggcagtggag gcgaaggaag gcgtcgaggt gaagggcgag acccagacct tcgccacgat
     1381 caccatccag aactacttcc ggatgtacga caagctggcg ggcatgactg gcaccgccga
     1441 gaccgaagag aacgagttct accagatcta cggcctcgag gtcagcgtca ttccgaccaa
     1501 caagccggtc cgtcgcaacg atcgacagga ccacatttac aagactcggc gtgagaagta
     1561 caacgccatc ctcgacgaag tcgagcggct ccacggtctc ggctggccga tcctgatcgg
     1621 aaccgtcacc gtggacgtct ccgagacact gagcaagatg ctcaagcggc gcggcctcaa
     1681 gcacgaggta ctgaacgcca agtaccacca gcgggaagcg gagatcgtcg cgcaggccgg
     1741 taaggtgaat gcgatcacga tcgcgaccaa catggccggc cgcgggaccg acatcaaact
     1801 ggaccccgcg ctcgacctgg ccaaggaaga agcgggcctc cagatcatcg gcaccgaacg
     1861 ccacgagtcg cggcgcatcg atcggcagtt gcgcggacgt tccggtcgcc agggcgatcc
     1921 cggtcagtcg atcttcttcc tgtcgctgga agatgatctg atgcggctca tcgggtcgga
     1981 tcggatcgcc cgctggatgg acaagagcgg cgcggaagag ggcgaggtca tcaccggcgg
     2041 cctggtcacc agcgccatcg ggaaggcgca gcagcgggtc gagctgcaga acttccaggc
     2101 acgcaagcga ctgctcgagt acgacgacgt gatgaaccag cagcgggagg tggtctactc
     2161 gctgcggctg ttcgctctcg agcggggtga ggaactcaag gcggaagccc agcggatgat
     2221 cgaggcggcg ctggagcgag ccacccgcca gttcctggtc gacgcggagc gcccggagga
     2281 atacgaccgc ggcgggctcc gcgagctgct caccatgcag tacctggtga gcgtcgaggc
     2341 cgtgctcgat ccgaagcaaa cgcctgagat cgagtcactt gtggcagccg ctcggactga
     2401 gggcgacgcc accttccacc gaaaaatcga ctatttgcag gagttcggcc gagccatcgg
     2461 aatccgggac gtggacgccc aggtgctctc gcaagtgatg ctgtcggtgc tggatgagaa
     2521 gtggaaagac catctctacg acctggacca gctccggaac gccattcact atcgcggttg
     2581 ggggcagaag gatccgctgg tggagtacaa gaaggaagcg tacgacatgt tcgaggactt
     2641 gatgcacgat atccagtcca cgttcaccga gcggttcctg aaagtgcagg tgagtgcgga
     2701 ccaaccgccc ccgcctcggc cgcagccgcc tccattgccg ccgacgccgg agtccaccgg
     2761 gcctgacgat ctgtttatgg gtgcgggtcc ggccacaaag atgccggctt cggtccccgc
     2821 accgtccggc gcggagaccg gcagatcgtc caccgtgacc agcagtctcg gcccggtggg
     2881 cagaaccgcg acagcggtgc cgccggtagg gcgaaatgac ccgtgtccct gtgggtcggg
     2941 taagaagtac aagaaatgtc acggagctgg tcagtaacaa gcggtgcagt tgcgctgctc
     3001 tcgctcgtga cctacgggct tcccgcccgc gcgcagaccg aggctgcggc gcgggcggtc
     3061 tctcttgatt ccgaggcgga gctcaagcag ttgcggcggg cacagcgcga cttcgagcgt
     3121 ctgcgggaat ccaatgcgcc ggcgctccga ttcgaccttc cgtcccgctg cgatctgtcg
     3181 ttcggcgtct actgctacca cttcaacacg cctgacgagg aaaccccgcc tgagccgccc
     3241 gagaccgatg cggcccggtc cgagttgctg gaacggctgg aagatggtgc ccgattgctt
     3301 cccggcgatc cctggatcgc ggg
//
LOCUS       DASRJQ010000208         2498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1877759, whole genome shotgun sequence.
ACCESSION   DASRJQ010000208 DASRJQ010000000
VERSION     DASRJQ010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1877759"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2268
                     /locus_tag="VFB89_04690"
     CDS             <1..2268
                     /locus_tag="VFB89_04690"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM26628.1"
                     /translation="SSAMDPNAIARCLQDCVTAAAPELAPWVSLLGVVLDISISPSSE
                     VAELEEQHRAERIPELYADLLERMVQTPVILVVEDSYLADSASEGVLAAVARRAQQQP
                     WLLFVTRDDRPTGWVPTATGRIELGPLDMTSSIELAEMATPERPLPPAIAEELAVRSG
                     GHPLLLRELARAAARGEDPDELPSTVEELAASQIDQLPPAQRSMLRRAAVLGDEFPED
                     LLLQMISDVAPEKSETELLAGLSGLIVSAGYLLRFRHPILREVAYAGLPYRRRRELHA
                     QAAEVLEANPAAADRRPELLALHYFVARNYDKAMDYGWRAGERAMARYAPAAAADAYR
                     RAAQAARLSSHVSPSERSFYLEALGDAEFLAGRSTEAAGAYLEALRGVSGQPLREAHL
                     ALKQILVEQRRGHYSNALRRASLGLRAVRDATDSEAGAARAWLQVRYASCRVQQGRYA
                     DARRWAERGLAEAEAAHELGAQAKARAVLAVVDMWSGRPMSKLHGEEALRIFEQLGDL
                     TEQAHMLNNMALQREAEGRWPDAALMLARAAEMYRRLGDASHAAGSDYNRAEVLVRQG
                     LLDEALPLLENSLRVARAVGDEDLVALVRKEMGRALSRAGDVTAGLILLEQARAELTR
                     LREPQEVVDADIASAEAHLLGGRPEQAMVLIEGAVTEAASIHAATLLPSAYRVQAAAL
                     FDMGQIVQARTALAQGLRQSSSPEVAHERGFLLAVAARIARQEDDPDAELVAEQAREA
                     LELLGVVRVPLPELS"
BASE COUNT          416 a          778 c          871 g          433 t
ORIGIN      
        1 agcagcgcca tggaccctaa cgccatcgcc aggtgtctcc aggattgcgt gacggccgcg
       61 gcgcccgagc tagccccgtg ggtctcactg ctcggtgtgg tgctcgacat ctcgatttcg
      121 ccgagctcgg aagtggccga actggaggag cagcaccgtg ctgagcggat acctgagctg
      181 tacgcagatc tgcttgagcg catggtccag actcccgtga tactcgtcgt cgaagattcg
      241 tacctcgcgg attcggctag cgaaggcgta ttggccgccg ttgctcgacg ggcgcagcag
      301 caaccctggc tgcttttcgt aacccgcgac gaccgtccca ccgggtgggt gccgacggct
      361 accggtcgta ttgagttggg accgctcgac atgacgtcgt cgattgaact ggccgagatg
      421 gcgacgccgg agcggccgtt accgccagcc atcgccgaag agctggcagt ccgctcaggt
      481 ggacacccgc tgctgctgcg cgaactggcc cgggccgccg cccgtggcga ggacccggac
      541 gagctgccat cgaccgtcga agagctcgcc gcgagccaga tcgaccagct gccacccgcg
      601 cagcgctcca tgttacggcg ggccgctgta ctcggcgatg agtttcccga ggacttgctt
      661 ttgcagatga tcagcgacgt ggcgcccgag aagtccgaaa ccgaactact cgccgggctg
      721 tcaggcctca ttgtctcggc cggttacctg ctccgcttcc gccaccccat tcttcgggag
      781 gtcgcgtacg ctggcttgcc ctatcggcgc cgccgcgaac tgcacgcgca ggccgccgag
      841 gttctggagg ccaacccagc cgcggcagac cgccgacccg agctcctcgc acttcactac
      901 ttcgtggccc gcaactacga caaggcgatg gactacggct ggcgtgccgg ggaacgggcg
      961 atggcgcgct atgcgccagc tgccgctgcc gacgcctacc gaagagcggc ccaggccgcc
     1021 cgactttcgt cgcatgtcag cccgagcgag cgttcgtttt acctggaagc gttgggcgat
     1081 gcagagtttc tggcgggccg atcgaccgag gctgcagggg cttatctgga agcactgcgg
     1141 ggcgtgagcg gtcagccact gcgggaggct catctggcgc tgaagcagat cctggtggag
     1201 caacgccgcg gccattacag caacgcgttg cgccgggcga gtctgggcct gcgtgctgtt
     1261 cgcgacgcga cagattccga ggcgggcgcc gctcgtgctt ggttgcaggt ccgctacgcg
     1321 tcctgccggg tgcagcaggg tcggtacgcc gatgcccgcc gatgggcgga gcgaggtctc
     1381 gccgaagcag aggcagccca tgagctggga gcgcaggcca aggcgcgcgc ggtactggct
     1441 gtcgtggaca tgtggtcggg cagaccaatg agcaagctgc acggggagga ggcgctgcgc
     1501 atcttcgagc agctcgggga cctcaccgag caggcacata tgttgaacaa catggccctg
     1561 cagcgagagg ccgaaggccg ctggcctgat gcggcgctca tgttggcgcg ggcggccgaa
     1621 atgtatcgtc gcctcggcga cgcctctcat gctgcgggct ctgactacaa tcgggccgaa
     1681 gtcttggtac ggcagggcct gttggacgag gcgctgccac tgctcgagaa ctcgctccgg
     1741 gtcgcccgtg ccgttggtga cgaggatctg gtggcccttg tacgcaagga aatgggtagg
     1801 gcgctgagcc gggcgggcga cgtaacagca ggactgatct tgctcgagca agctcgcgct
     1861 gaattgacca gactccgaga accgcaagag gtggtggatg cggacatcgc gtccgcggaa
     1921 gcgcacctgc tggggggtcg gcctgagcag gctatggtcc tgatcgaggg agcggtgacc
     1981 gaagctgcct ccatccacgc cgccactttg ttgccgtcgg cgtaccgcgt ccaggccgct
     2041 gccctgtttg acatgggcca gatcgtgcaa gctcggacag ctttggctca gggtttgcgg
     2101 cagagctcct cacccgaggt cgcgcacgag cgcggattcc tgctcgctgt ggctgcacgg
     2161 atcgcacgtc aggaggatga tcctgacgca gaactggtcg ccgaacaggc gcgcgaggct
     2221 ctggagttgc tgggcgtcgt ccgggtgccg ttgcctgaac tgagttagcc gagtgagtca
     2281 gggagcccgg taggtcgacg gcgtagacag ggcacccgga cgaagccctc aggttgctgg
     2341 acgtcgtccg ggcgccgctg ccggcgttcg tcgggtgagc tagaagtggg aacggccgtc
     2401 gacattgaag aggaaaggca agaagaggtc gccatcgcgt cggaagctct gcttgtaatc
     2461 gacgcacgcc tcttggtctt tgccaatgag acccttct
//
LOCUS       DASRJQ010000209         3555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3248531, whole genome shotgun sequence.
ACCESSION   DASRJQ010000209 DASRJQ010000000
VERSION     DASRJQ010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3248531"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VFB89_04695"
     CDS             complement(<1..430)
                     /locus_tag="VFB89_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26629.1"
                     /translation="MTPPIILVVTGASGAGKTTLVRGLEARSLLGVSCHYFDSIGVPP
                     REVMERDFGGGMGWQEAMTRTWIERLSLRPDRVAVLDGQTRPSTVRAAFHLAGIREGA
                     IVLIDCAAAERNRRLHGDREQPELASAQMDTWAAYLKGQAD"
     gene            641..1051
                     /locus_tag="VFB89_04700"
     CDS             641..1051
                     /locus_tag="VFB89_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26630.1"
                     /translation="MPRIHRFTLGLTLLNFVLLALLVLSQARTALAKQAVPAVLRGRA
                     LEIVDNQGRVRANILVHGPETVNGVTYPENVLFRMIDPSGGPLVKLTVAPNGSALGLS
                     DGLSGNGGVQLYARDTASFVRIVDKSGRTQMLKP"
     gene            complement(1117..1557)
                     /locus_tag="VFB89_04705"
     CDS             complement(1117..1557)
                     /locus_tag="VFB89_04705"
                     /inference="COORDINATES: protein motif:HMM:NF020177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1772 domain-containing protein"
                     /protein_id="HZM26631.1"
                     /translation="MVHLIAGAATLLCTLFTGAAIYINLVEHPARLSVGTEAAARQWA
                     PSYRRATVMQATLAVSAAAAGVVTWLHGEGIAWLWGGFVMLAIVPFTLLVIRPTNDRL
                     LAQGRDLASAETRQLLERWGWLHGVRSALGLVASVIYLYALAHL"
     gene            complement(1654..2124)
                     /locus_tag="VFB89_04710"
     CDS             complement(1654..2124)
                     /locus_tag="VFB89_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26632.1"
                     /translation="MHAWSSRRARFAILFTASATFGACTGTEQPVAVNVVATEYAFVS
                     PDSTRSGPAVFTLANRGTKDHELFIGLLRPGATASQIAAAHQQGVSFRQLPKIYLDGA
                     ASVALIASPGTTSPARVTLDLLPGRSYVLFCQRRDSVGAPQHAALGMFRLLRVN"
     gene            complement(2315..2743)
                     /locus_tag="VFB89_04715"
     CDS             complement(2315..2743)
                     /locus_tag="VFB89_04715"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZM26633.1"
                     /translation="MEHIRTYLLRAFLSAGVVAIGPATAAQQQRPSASSVTRTDLLKR
                     VLPAGNFRNVQASVIELGPGAAAPRHRHDVAVLAYVVEGTVENQFGGGATQIHKAGDS
                     WWEEPGTVHDVARNASKTARARLLVVYIGEEGKAATVPIR"
     gene            complement(2852..>3555)
                     /locus_tag="VFB89_04720"
     CDS             complement(2852..>3555)
                     /locus_tag="VFB89_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM26634.1"
                     /translation="DAHSWRGVVLAQYEWNWAEAELEFRRAIELNPNYAFAHDQFALA
                     LAFTGRFDESVAESRRAAELDPLSPQVLIDAGMAPMFEKNFPAAKELARGAGELDPTY
                     FFPVMMEGWADLEAGKFREAVPFLKKATTMGAPPFVTAYLGYAYGAGGDRASAMATLE
                     ELKKLSKGGQVLPFNLALVYLGLGDHGRALDNLERALAADSQMMAWLGRDAIFDPLRA
                     EPRFMALMKKLNFVE"
BASE COUNT          656 a         1172 c         1120 g          607 t
ORIGIN      
        1 catccgcttg ccctttcaga tacgccgccc aggtatccat ctgtgccgag gcgagttcgg
       61 gctgttcccg gtcgccatgc aatcgccggt tgcgctccgc tgcggcacaa tcgatcaata
      121 caatcgctcc ttcacgaatg ccggccaggt gaaaggcggc tcgcactgtg ctgggtcgag
      181 tctgaccgtc caagactgcc acgcgatctg ggcgtagcga caggcgttcg atccaggtgc
      241 gggtcatggc ttcctgccac cccattccgc cgccgaaatc tcgttccatg acctcccggg
      301 gaggaactcc gatcgaatcg aagtagtgac agctgacgcc aagcagactg cgggcttcca
      361 ggcctcgaac cagtgtagtc ttgccggcgc ccgaagcccc ggtaaccacg agaatgatgg
      421 gcggggtcat tcggggcccg ggcgtgaaag gcaactgggt gccgcggtcg aacgactcac
      481 gtttctgccg cgggggcgta ccgaggccgc cgcagtgcac caagagcaaa gatatcctgc
      541 ggaggtaagg cacgcacgct ctgtaaactc ctgccggtct tccggcatca catctgcgag
      601 ctgctccctg ccgcaccacg ctttcgcgag gacttgtgcc atgccgagaa ttcatcgttt
      661 cactctggga ctcaccctcc tcaactttgt gctcctcgcg ctgctggtgt tgagccaggc
      721 ccgcacggcc ctggccaaac aagccgtacc ggctgtgctt cggggccgcg ctctcgagat
      781 cgtcgacaat cagggccggg tacgggccaa tattctcgtc cacggccccg aaacggtgaa
      841 cggcgtgacg tacccggaga acgttctctt ccggatgatt gatcccagcg gcggcccact
      901 ggtgaagctc accgtcgccc caaacggctc cgccctcggt ctctccgacg gtctctcggg
      961 gaacggtggc gttcaactct atgctcgcga taccgcgagc ttcgtgagaa tcgtggacaa
     1021 gtcgggacga acgcaaatgc tgaagccata gcggccaccg atgcttgccc atgtcggcgg
     1081 cagtcgccac ctgctgcatc tgtggcggtg gcgtggctag aggtgggcga gcgcatagag
     1141 atagatgacc gacgccacga ggcccagcgc gctgcgtacc ccgtgaagcc aaccccagcg
     1201 ctcgagcaac tggcgggtct cggccgaggc caggtcccga ccctgggcga gaaggcggtc
     1261 gttagtcggg cgaatcacca ggagcgtaaa gggcacgatc gcgagcatca cgaacccgcc
     1321 ccagagccag gcgatcccct ctccgtggag ccaggtcacg acgccagctg ccgcggcgga
     1381 caccgcgagc gtcgcttgca tgacggtcgc ccgccgataa ctgggagccc actgacgagc
     1441 ggccgcctcc gtcccgaccg acagcctcgc agggtgctcg accaggttga tgtaaatggc
     1501 tgccccggta aagagcgtac acaacagagt cgccgcgccc gcaatgagat gaaccatcac
     1561 gacctcaccc gaagcagggg atagtcttca gagccgatcg cgaggaatca ctcgagacga
     1621 aagcaggcag cagcagcgcc agtattgcga cggtcagttg acccgcagta agcggaacat
     1681 ccccagggcc gcatgctggg gggcgcccac ggagtcgcga cgctgacaga acaggacgta
     1741 cgatcgaccc ggcagcagat ccaatgtcac gcgtgcgggg gaggtcgtac caggcgaggc
     1801 gatcaacgcg acacttgcgg caccatcgag gtagatcttg gggagttgcc ggaagctgac
     1861 gccctgctgg tgggcagctg cgatctgcga ggccgtcgcc ccgggacgga gcagcccaat
     1921 gaacagctcg tgatccttcg tcccgcgatt cgcgagcgta aagacagcgg gaccggaccg
     1981 cgtggagtca ggactaacaa aggcatactc ggtggcgacg acgttaacgg caacgggctg
     2041 ttcagtgccc gtgcacgcgc cgaaggttgc cgacgctgtg aagaggatcg cgaatcttgc
     2101 tcgacggctc gaccaagcgt gcaatgaagc ctcctgcttg cggcctaacg gcctgcgctg
     2161 ctgctgccgg gcgtgccgaa gccaccgcag gccaagttca aagatacgtc gcggcggctg
     2221 gggcgctcac gccccggcag cagctagccc ctggttcggc gcgcatcccc agggtgccgc
     2281 tcgaagactg gtattcaaga gatgcaggcc gttctcacct gattggaacg gttgcggctt
     2341 tgccctcctc gccgatatag acgaccaaca gtcgcgcgcg cgcggtcttg ctcgcgttcc
     2401 gcgcgacgtc gtgaaccgtt cctggctcct cccaccagct atcgcctgcc ttgtggatct
     2461 gggtcgcacc cccaccgaat tgattctcca cagttccttc gaccacatat gcgagcacgg
     2521 ccacatcgtg gcgatgtcgg ggcgccgcgg cacctggtcc gagctcaatg acactggcct
     2581 gaacattccg gaaattgccg gccggcagaa cacgcttgag gagatccgtc cgagtcaccg
     2641 aactcgcgct cggtcgctgc tgctgcgctg cggtcgccgg tccaatggcg acgacaccgg
     2701 ccgagaggaa tgccctgagg aggtaggttc tgatgtgttc catgacactg tcctccttgc
     2761 cggaacttgg atcgaacgag atgcggtctt tcgtcagcag tgcggcctgg gtggcgacga
     2821 ctaccctgtc ttcggtactc cccagagcca ttcactcgac gaagttcaac ttcttcatca
     2881 gcgccatgaa ccgcggttct gcgcggagcg gatcgaagat ggcgtctcgt ccgagccacg
     2941 ccatcatctg ggaatcggcg gcgagcgccc gctccaggtt gtcgagcgcc cgcccgtggt
     3001 cgccgaggcc cagatacacc agcgccagat tgaagggcag gacctgccca cccttcgaga
     3061 gtttcttgag ctcttcgagc gtcgccatcg cgctggcccg gtcgccgccg gcaccgtagg
     3121 cgtacccgag gtacgcggtg acgaagggcg gcgcgcccat cgtcgtggcc ttcttgagga
     3181 acgggacggc ctcgcggaac ttgccggctt ccaggtcggc ccagccttcc atcatgacgg
     3241 gaaagaaata ggtggggtcg agctcccccg ctcctcgcgc cagctccttg gccgcaggga
     3301 agtttttctc gaacatcggc gccatgccgg catcgatcag cacctgcggc gacagggggt
     3361 ccaattcggc cgcgcgccgg ctctccgcga cggattcgtc gaatcggccg gtgaacgcca
     3421 gcgccagggc gaactgatcg tgcgcgaacg cgtagttcgg gttgagctcg atggcccggc
     3481 ggaactcgag ctcggcctcg gcccaattcc actcgtactg cgcgagtacc actccgcgcc
     3541 acgagtgagc atccc
//
LOCUS       DASRJQ010000210         2729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1835824, whole genome shotgun sequence.
ACCESSION   DASRJQ010000210 DASRJQ010000000
VERSION     DASRJQ010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1835824"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2381
                     /locus_tag="VFB89_04725"
     CDS             <1..2381
                     /locus_tag="VFB89_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26635.1"
                     /translation="LREAASRQVQLNEAVRTFAPGPLLSNIQDLKREMGELKALEQSG
                     FLGRILSFFSKGISGLGGLTGLGRGPAEIDIQKQRLAYEQAAGPGRESELLGISGDLA
                     KLAQQRAALYGTPAEQFAAINEITRGETRRAEIARDQAILQGPGTMGFREHQEAIFAG
                     RSRLFGGQAGLARRQISDALFERFYLGGGEEQYQPGQGLEQVSAGMAASAPIDISRAQ
                     QIGEDIRRQIIDEITGQMLRENAEALSGGAEQIGVGPTASELAAAAPRFQAARVQELD
                     VQKQLFDVRSSFVGLTREEREQLEKITIEMQKQADIAAAGNDEALKDLAEARAAMAQI
                     NLELLKIERTDAFAGLAKGVKSVGEEWSATGKTMQDFARSTASSMQRSFSDLFFNAMT
                     GNFKSLADVGKSFMLSIARDISNSLSQQLTGSIYQGLGSFFPNQSPGSPGFFGNAVTG
                     SAVTAGQGGVAGISQAAGFGTGYAGYFTGSSPEAGAPGGGFGVGLGEGEGVGYGSALA
                     GGAVGGIVGAAGIGLQYTGSKRDARAGQGILTGASLGGSVGGIYGMAIGAIVGLFASA
                     ATQDLKDREEATKAGIHRGDAFNIRRLQPYLTGDIQRVQSASTFLELAGAIQGAMHPA
                     FGGPSSRGGGRRGGVAGFAVSIDGETVVLPGGSYPVTMTLTEFADALRDNGDTFRAAL
                     QLGINEAANRQISDAYAQAVRDRVSILGTFESFPVAYSEVGVGIERRTILPVGALDRV
                     QPGTQNLSISRVLLSSTGLVDADTDMLIRRIRSRNSQTDAIRELDTEPFYRT"
     gene            2378..>2729
                     /locus_tag="VFB89_04730"
     CDS             2378..>2729
                     /locus_tag="VFB89_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26636.1"
                     /translation="MTISGAIKPKAYYPRFKWGSPTVTTDIVEPAVIAMTRNETGLRG
                     QNIVASGKAEYLFGRIEEAAQLQLMCEQDQLAALRWFTRDWAGQGKQFQAWIDRFTGS
                     CWLFENNLKDQNGLA"
BASE COUNT          503 a          834 c          926 g          466 t
ORIGIN      
        1 tcctgcgcga agccgcgtcc cgccaggtgc agctcaatga agctgtccgg accttcgcgc
       61 ctggtccgct cctcagcaac attcaggacc tcaagcgcga gatgggcgag ctcaaggcgc
      121 tcgagcagag cggcttcttg ggtcggatcc tatcgttctt ctcgaaaggt atctcgggac
      181 tcggcgggct cacgggcctt gggcgtggtc cggctgagat agatattcag aagcagcggc
      241 tcgcctatga gcaagcggct gggcctgggc gcgaatcgga gctcctcggg atatccggtg
      301 atctggccaa gctcgcgcag cagcgggccg cgctctatgg gaccccggcg gaacagttcg
      361 cggcgatcaa cgagatcacc cgcggcgaga ctcgtcgagc ggagatcgcg cgtgaccagg
      421 ccatcctgca gggtcccggc acgatgggct tccgcgagca tcaggaagct atctttgccg
      481 ggcgctcccg actcttcggg ggtcaggccg gcctcgcccg tcgccaaatc agcgacgcgc
      541 tattcgagcg cttctacctc gggggcggtg aggagcagta ccagccgggt cagggcctcg
      601 agcaggtgtc ggctggtatg gccgccagcg cgccgattga tatcagccgg gcgcagcaga
      661 tcggcgagga catccggcgg cagatcatcg acgagatcac cggtcagatg ctccgcgaga
      721 atgcggaggc gctgagtggt ggcgctgagc agatcggcgt cgggccgaca gcgtctgaat
      781 tggctgccgc agcgccccgg ttccaggccg cgcgagtgca ggaactcgac gtacaaaagc
      841 aactgttcga tgtgcgctcg tcctttgtcg gactgacgcg ggaggagcgc gagcagctcg
      901 agaagatcac gatcgagatg cagaagcagg cggacattgc agcggccggc aacgatgagg
      961 cgctgaaaga cctcgctgaa gcccgtgcgg cgatggcgca gatcaatctc gagcttctga
     1021 agatcgagcg gacggatgcc ttcgccgggc tcgcgaaagg tgtcaagagc gtcggcgagg
     1081 agtggtcggc gacgggcaag acgatgcagg acttcgcccg aagcacagcc tcgagcatgc
     1141 agcgctcgtt ttcagatctg ttcttcaacg cgatgacagg caacttcaag agccttgctg
     1201 acgtcggcaa gtccttcatg ctgtccatcg cgcgcgacat ttccaactcg ctctcgcagc
     1261 aactgacggg ctctatctat cagggcctcg gcagcttctt cccgaatcag tcgccaggat
     1321 ccccaggttt tttcggtaat gccgtcaccg gctctgcggt gacggcaggc caagggggcg
     1381 tggccggcat ctcgcaagca gcgggattcg gcactgggta cgccggatac ttcacgggct
     1441 catcgccaga agccggagcc ccaggcggcg ggttcggggt tgggctgggc gagggcgaag
     1501 gcgtcggcta tggctcagcg ctcgccgggg gtgcggtggg cggcatcgtc ggggccgccg
     1561 ggattgggct tcagtacacg ggcagcaaac gagatgcgcg ggcgggtcaa ggcatcctca
     1621 ccggagcatc gctcggcgga tcagtcggcg gcatatatgg catggccatc ggcgccatcg
     1681 ttggtctctt cgcgtctgcg gcgacgcaag atctcaagga ccgagaagag gcgaccaagg
     1741 ccgggatcca tcgcggcgac gccttcaaca tccgtcgcct gcagccatac ctgacgggcg
     1801 atatccagcg ggtccaaagc gcctccacat tccttgagtt ggccggggcg attcagggtg
     1861 ccatgcatcc ggcctttggt ggtccctcat cccgcggcgg cggccgacgt ggcggcgtgg
     1921 caggattcgc ggtctccatt gacggcgaaa cggttgtact gccgggtggg tcctatcccg
     1981 tcacgatgac gctgacggaa ttcgcggacg cactccgcga caacggcgac accttcaggg
     2041 cagcgctgca gctcgggatc aacgaggcgg cgaatcgaca gatcagtgac gcttatgcgc
     2101 aagccgtgcg ggaccgggtc agcatcctcg gcacgtttga gagtttcccc gtggcctata
     2161 gcgaagtcgg cgtcgggatt gagcgacgca cgatcctgcc ggtcggtgcg ctggatcgcg
     2221 tccagccggg cacgcagaac ctctcgatca gtcgggtgct cctgagctcg acgggcctgg
     2281 tcgacgccga taccgacatg cttattcgcc gcatccggtc tcgcaatagc cagactgacg
     2341 cgatccgcga gctcgacacg gagccgtttt accggacatg accatctccg gcgcgatcaa
     2401 gcccaaggcc tattacccga gattcaagtg gggctcgccc acggtgacga ccgacatcgt
     2461 tgagcctgcc gtgatcgcca tgaccaggaa tgaaacggga ctgcgcggcc agaacatcgt
     2521 ggccagcggt aaggccgaat acctgttcgg ccgcatcgag gaggcggcgc aactgcaact
     2581 gatgtgcgag caggaccagc ttgcggcgct ccgctggttc acgcgcgact gggcgggcca
     2641 gggcaaacag ttccaagcct ggatcgatcg cttcacaggc tcctgctggc tattcgagaa
     2701 caacctgaag gatcagaacg ggttggcgc
//
LOCUS       DASRJQ010000211         2087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_4094728, whole genome shotgun sequence.
ACCESSION   DASRJQ010000211 DASRJQ010000000
VERSION     DASRJQ010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4094728"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VFB89_04735"
     CDS             <1..328
                     /locus_tag="VFB89_04735"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZM26637.1"
                     /translation="QLQQVILNLLRNAFEAMAEVYDRPRQLVVRTEPESDTGVRVSVR
                     DSGVGLDRHSLDKLFDAFYTTKPGGMGIGLSVSRSIIESHHGRMWVEPNDGPGATFAF
                     SIPVRA"
     gene            553..840
                     /locus_tag="VFB89_04740"
     CDS             553..840
                     /locus_tag="VFB89_04740"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HZM26638.1"
                     /translation="MATVKDPVCGMIIDSESATARAEVAGQVVYFCSSECAAKFEANP
                     ERYAPDARAAEERLEQHEPPYTTLGFLTSPKFGAAGSGGLEYERLPEAHDR"
     gene            complement(1013..1501)
                     /locus_tag="VFB89_04745"
     CDS             complement(1013..1501)
                     /locus_tag="VFB89_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26639.1"
                     /translation="MRPTVFSEGFFLQLAAPGARASDELALPFGAGKTSPISAVDVAR
                     AAAVILSDPAPHIGRVYDLTGPESADLEHYARVFSEALGRPIRYRDVPLPAWGESLRQ
                     AGFSDYLVRHLSAMAELTRQGRYDRMTDTMCKLTGEAPTSMRDFVKLHAAEFEQRVPG
                     HS"
     gene            1538..1681
                     /locus_tag="VFB89_04750"
     CDS             1538..1681
                     /locus_tag="VFB89_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26640.1"
                     /translation="MSVVSVMLICVTVICDMFTKASTPWWRATAAVLIVASRKAGETD
                     IPK"
BASE COUNT          367 a          655 c          697 g          368 t
ORIGIN      
        1 ccagctccag caggttatcc tgaacctgct tcgtaacgcc ttcgaggcga tggccgaggt
       61 ttacgatcgt cctcggcagt tggttgtcag gaccgagcca gaaagcgata cgggggttcg
      121 cgtcagcgtc cgggattccg gcgtgggact cgaccgccac agcctcgaca agctgttcga
      181 tgcgttctat acgaccaagc ccggagggat gggcatcggg ctgtccgtca gtcgttcgat
      241 catcgagagc catcatggcc gcatgtgggt cgaaccgaac gacggtccag gggccacgtt
      301 tgcgttctcc attcctgtcc gcgcctgaac ggcagcgccg ccacgttagg tgcggtatct
      361 cggacactcg acgacacaga aacctgccta tcccaccggc actgcgcagt cgcctcggct
      421 cctggcgtat cactggccgg gcaacatccg ggctgaaaac gagcacgctt cgatcgcgaa
      481 tggagaagct cggcttgctc tagggatcgt ttccaacgat cgttggattg gtgcgatgga
      541 caggaggtat ctatggcaac cgtgaaagac cccgtctgcg ggatgatcat cgacagcgag
      601 tcggcgacgg ctcgcgcaga ggtggcgggg caggtggtgt acttctgctc gtcagaatgc
      661 gcggcgaagt tcgaggcgaa cccggagcgc tatgcgccgg acgcaagggc tgcggaggag
      721 cggctcgagc agcacgagcc gccgtatacg actctcggct tcctgacgtc gccgaagttc
      781 ggggctgcgg gcagcggagg gcttgagtac gagcggcttc ccgaagctca cgaccgctag
      841 ctgcctaacg cgcacgtgtt ttggccaacc accagagctg cccacggccc cgcactccgc
      901 accgaccacc caggcgacgg cacgcatcgt ggtcgtcggt gcggagtggg tcgattcgct
      961 ccctagcgcc cgtgatcagg gagcccacct gcgtttcgac gtggtgagcc cctcaggaat
     1021 gccccggtac acgctgctcg aactcagcgg catggagctt cacgaagtcg cgcatgctcg
     1081 tcggcgcttc gccggtgagc ttgcacatgg tgtccgtcat gcgatcgtaa cgcccctgcc
     1141 tggtcaactc ggccatggcc gagagatgcc taacgaggta gtcggaaaac ccagcctgcc
     1201 gaaggctttc gccccacgcc gggagtggga cgtcgcgata tcggatgggc ctgccgagcg
     1261 cctcggagaa gacgcgcgca tagtgttcca ggtcggccga ttccggcccg gtcagatcgt
     1321 acactcgtcc gatatgcggt gcggggtcgc tgaggatcac tgctgcggca cgcgccacgt
     1381 cgaccgcgga gatcggtgag gtcttgccgg cgccgaatgg cagcgccaac tcgtcggagg
     1441 cccgcgcgcc gggggcggcg agttgaagaa aaaagccctc ggagaaaaca gtcggccgca
     1501 ccgtgacgac cggcagtctc gaccaggaca gagcctggtg tcggtggtct cggtgatgct
     1561 catctgcgtg accgtcatct gtgacatgtt cacgaaagcc tcgacaccgt ggtggcgcgc
     1621 caccgcggcg gtgttgatcg tggcttccag gaaggccggc gagacagaca ttccgaaata
     1681 gaggggcccg cagccttcga tggcgcggtg catcgaggcg aggtccgtca ggtcgccttg
     1741 tacgacctcc gcgccgagcc gccgtagggc tttggcgcgc tcgtcctcac gccggaccaa
     1801 ggcgcgcacc gtgtgccccc ttgcgagcag gaattcggtg aggttgcggc gccgatgccg
     1861 cccacagcgc cggctgctcc ggtcacgagg atcgggttgt cgtgtttagg cgcgcgaatg
     1921 gaggtcgtta tggcatcgct ccggtgcatg gccgaagatc gcactgcaag gagtcaacgc
     1981 ctgcacttat acatcgggcg cggggcgact ccattacacg atggtatcgc cccagcactc
     2041 agacgaccgg ctgttcgtgc acccttcccg tgggcgtctg accggcc
//
LOCUS       DASRJQ010000212         6582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1270233, whole genome shotgun sequence.
ACCESSION   DASRJQ010000212 DASRJQ010000000
VERSION     DASRJQ010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6582
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270233"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..587)
                     /locus_tag="VFB89_04755"
     CDS             complement(156..587)
                     /locus_tag="VFB89_04755"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZM26641.1"
                     /translation="MDELKIREMEQDGDFAVWFSELVAETEEAAGWGNPINDRYLVLS
                     NEIGDWIGGLRFSLRGGVANLLDVAVVETERHRGHAHRLMAAFEERSAEAGAHLAEFW
                     TDDLGSEGLLAAMGWRVVLRRPGYIGGRTWVLMEKSLGDEE"
     gene            808..1221
                     /locus_tag="VFB89_04760"
     CDS             808..1221
                     /locus_tag="VFB89_04760"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZM26642.1"
                     /translation="MTVACAFCGVLNRVDLERLSAGPKCGACGRPIRLDRPVKLTEAD
                     FDRVIGAAGVPVLLDCYADWCGPCRAMAPALDEFARARTGQVLVLKLDTDASPVISHR
                     LNIRGIPTLIAFRQGKELRRHVGMADLPVLESLIQ"
     gene            complement(1250..1519)
                     /locus_tag="VFB89_04765"
     CDS             complement(1250..1519)
                     /locus_tag="VFB89_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26643.1"
                     /translation="MRLSSIVTLIAVVLFAAFLLYSTLASQRVQCAVTVEYEGRRNAA
                     TASAATEREAEQQARTTACGPIAAGMNATIACTNRTPVKRDCRTL"
     gene            1754..2167
                     /locus_tag="VFB89_04770"
     CDS             1754..2167
                     /locus_tag="VFB89_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZM26644.1"
                     /translation="MTVSTARPGTFLVVDDSALHHQMYRLIFSKGPLAGSTVHHAMNG
                     REGYALLAAHPEITVVLLDLNMPEMNGLEFLARRNAEKLHLHVPVVLVTTESTPEDET
                     RGRAAGAWDYLRKPFQPSDVERLVARLQAERAPAA"
     gene            2170..3819
                     /locus_tag="VFB89_04775"
     CDS             2170..3819
                     /locus_tag="VFB89_04775"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HZM26645.1"
                     /translation="MSHDHAFGELIQEYIAECLPLAEAVNDACLGLERRWAAGDLDAG
                     AVTGLKGTLHTLKGNSAMMGFRPMQVVAHALEDLAARVAKDSVGRGEVEAGLLVEGSG
                     LLVDLIRAAADPEGGGPAADAFVERVRSRLESAVSSTQTTPLRLDRRRTERRTENGID
                     GGTVRVDFRRLDNLLEIFGEAMIEQSALADVYRRLAARLGSSPELTDLDRVVVALGAT
                     MKRLETALMQTRLLPLSTVFGRFTRSVRDLARDQRKKVRLVIEGGDTLLDKTILDRLG
                     EPLVHLVSNAVVHGVESESERARVGKAPEATVGLRAVALADRVSITVSDDGRGLDATR
                     ILAKARELGLEAPSAATADVYSLIFVPGFSTAERVSHIAGRGVGLDAAAAAIHDLGGS
                     IEVASRPGQGTVFTLSLPLTLAIVRSLIVEVDRERYAVPLSHVAETVRLEAGTLHEIN
                     RQGVLMWRGSAIHVADGGRLLGTGSSRSAPRNYFVVLFAGPRRRGLLVDRLVGHQDVV
                     VKSLDPSLGRPDVVSGATILGDGRVACILDAAGIVAQREAA"
     gene            3821..4393
                     /locus_tag="VFB89_04780"
     CDS             3821..4393
                     /locus_tag="VFB89_04780"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HZM26646.1"
                     /translation="MARNSAKFSVPVAPPPSPPPGPTDVFQFADQLAADASNIQAPRP
                     VTTLHLITFRLDGEEFGLPVETVREVIRVGEITRVPQSPPHIRGVTNLRGRILPVVEI
                     RSRLGLAPLVPRSTARIIVAEVRGRVLGLLVDSVAQVQKVPAERVVATPDEVKSGHSD
                     YLIGVVQLYERLLILLDLPRALEATGDSHS"
     gene            4401..5876
                     /locus_tag="VFB89_04785"
     CDS             4401..5876
                     /locus_tag="VFB89_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HZM26647.1"
                     /translation="MTQTIMQKIRDWTIRRKILTGFAAVLLLTSIQGWFAIRQLERLA
                     DTSGAALQAFGNTRSIILLTTIVTVAVGLALAMLLSRLIANPLESLGILAEEVSKGNL
                     AADIRSQSRDEIGWLEHSMRQMVKNLRLVVGQISTSSRTVASSAEEISASSVQMAKGA
                     EAQTNSTEETSATMVEMAAQMQALARNAEALAASVDETSSSIQQMSATLVQTAQNGET
                     LLQSVDEAMSRLNGMIENIGAIASRVHMVDEVSQRSVTEAKSNGEQLQVSINSISTQS
                     EEIGKIVKVIEGIADQTNLLALNAAIEAARAGEAGRGFAVVADEVRRLAERSVSATQE
                     IGAVIESVQRETRAAVELMGGVLGAIVGSIDKTSHLVGEAARATEEHALGAKEVLRMA
                     GSMASLTRQIAASVRENAVGAREITTAAQKMNQLTHQMSEAVSEQKRGGEMVVKAVES
                     VALVSRQNLVAVEQMSVAAKTLAGESELLKQRVETFQLPAA"
     gene            5885..>6582
                     /locus_tag="VFB89_04790"
     CDS             5885..>6582
                     /locus_tag="VFB89_04790"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZM26648.1"
                     /translation="MSRAGAAERLAALTAATGEERQALVLAAFEDEAPSVREQAIRLA
                     TRYVEPEILGGLVADGVNASRRNGALAALERQGPYAVPYLVKLLSSQDGELVMFALQI
                     LARIGDAAAAGGMLPLLHHADPNVAQAAVEALGRLRATGAVPALLEQLRGNLWLQLAA
                     VNALGEIGDPAAVPALLSLVPDSFVAEQAVRSLQRIAAPDSLEPLLSVLASVHERPLR
                     DAILEALGVVLDLHP"
BASE COUNT         1110 a         2143 c         2157 g         1172 t
ORIGIN      
        1 cttggaaaac atctcgcgag cggattctgt gagtcccatg tgctctttcc agggaagaga
       61 agtgcggggc cgaacaaacc ccgcagctga agacgcaaag accagccgcc aaaattacgc
      121 ggctggtcgc tttcgtgcca atcgggcggt tgactttact cttcgtcgcc taacgatttc
      181 tccatcagca cccaggttcg gccgccgatg tagcctgggc gccgcaacac tacccgccag
      241 cccatcgccg ccagcagccc ctcggatccg aggtcatctg tccaaaactc ggccaggtgc
      301 gctccggctt ccgccgagcg ctcttcgaag gccgccatga gccggtgggc gtgaccccga
      361 tgccgttcag tctccaccac agccacgtcg agcagattgg cgacgccgcc ccgcagcgag
      421 aatcgaaggc ctccgatcca atcaccgatc tcgttgctca gcacgaggta acggtcattg
      481 atcggattgc cccagccggc agcctcttcc gtttccgcta ccagctcgga gaaccaaacg
      541 gcaaagtcgc cgtcctgttc catttcgcgg atcttgagct cgtccatggt gactgtgctc
      601 cccaggcact acctccagtc aacgcaacag cagtgccaaa ctgtctgacc ccttccagcc
      661 actagcctct gtggggtaaa acgcatggca gacccggtgg gttcagttat cgtacgacgg
      721 gcggggtgca tggagatccg ccgttctggc ctgcaacgat tggcgcgaga ctgaggtatg
      781 agcgcgacga caaatacggg gcgaccagtg acagtcgcct gtgcgttctg tggcgtgttg
      841 aatcgagtgg acttggagcg cctctcggca ggccccaaat gcggtgcttg cggtcgcccc
      901 attagactcg atcgaccggt caagctgacc gaggccgatt tcgaccgggt cattggtgcc
      961 gcaggtgttc cggtcctgct cgactgttac gccgattggt gcgggccctg tcgcgccatg
     1021 gcgcctgccc tggacgagtt cgccagagcc cgaaccgggc aggtgctggt tctcaaactc
     1081 gacaccgacg ccagtccggt catcagtcac cgcctcaaca ttcgcggcat tccgacactg
     1141 attgcgtttc ggcaggggaa agaactccgc cggcacgtcg ggatggccga tctgcccgtg
     1201 ctcgagagtc tgattcagta gcagccgtca ggatgcgacg gagcccgtct cacagagtgc
     1261 ggcagtcgcg cttgacgggc gtacggttgg tgcaggcgat ggttgcattc attcccgcgg
     1321 cgatcgggcc gcaggcagtg gtccgtgctt gttgctctgc ctcgcgctcg gtcgccgcgg
     1381 acgcagtggc cgcgttgcgc cgcccctcat actccacggt cacagcgcac tgcacccgct
     1441 gtgacgcgag cgtcgagtac aggagaaagg cggcaaagag aacgacagcg atgagcgtga
     1501 cgatgctgct gaggcgcatg gggcgatgaa tatcctacgc gccgtcccgc gccgccagag
     1561 cgaacccgct ccggcatctt gcggcaacac ctgccgtggt agcctccaat cactcttcta
     1621 cagcgtgagg tgccaatcgc ttagctgatt gtaacgggct ggtatgtgat ttgccccttc
     1681 ctccaaagct ttccgacccg aagtcgtttt ccgtaagccg ccttggcgca tggcgcgttg
     1741 gaggacgtcc cccatgacgg tgtcaacagc tcgtcccggc acgttcctcg tcgtggacga
     1801 ctccgcgctc catcaccaga tgtaccgcct gatcttctct aaaggacctc tggcgggcag
     1861 cacggttcat cacgcgatga acggccgcga gggttacgcc ctcctcgccg cacatccgga
     1921 gatcaccgtc gttctcctcg atctcaacat gcccgagatg aacgggctcg agttcctggc
     1981 tcgccgcaat gcagaaaagc tgcacctgca cgttccggtc gttctggtga cgacggagag
     2041 cacaccggaa gacgagaccc gcgggcgtgc ggccggggct tgggactacc ttcgcaaacc
     2101 cttccagcct tccgacgtcg agcgccttgt tgcacgcctt caggccgaac gcgccccagc
     2161 tgcctgacca tgtcgcacga tcacgcgttc ggcgagctga tccaggaata catcgccgaa
     2221 tgcctcccgt tggccgaggc ggtgaacgac gcgtgccttg gcctggagcg gcgctgggct
     2281 gccggtgatc tcgacgcggg agccgtcacc ggtctcaagg gcacgctcca caccctgaag
     2341 ggcaattcgg cgatgatggg cttccggccg atgcaggtcg tggcccacgc gctggaagac
     2401 ctcgccgccc gggtggcgaa ggactccgtt ggccgaggtg aggtcgaggc cggcctgctg
     2461 gtcgagggat ccggtttgct ggtggatctc atccgggccg ccgccgatcc cgaaggcgga
     2521 ggccctgccg ctgatgcctt cgtcgaacgg gttcgctccc gtctcgagtc ggctgtttcg
     2581 agcacgcaga ccacgccgct ccggctcgac cggcgccgga ccgagcgtcg aaccgagaac
     2641 ggtatcgacg gcgggacggt gcgggtcgac ttccgccgcc tggacaatct gctcgagatc
     2701 tttggcgagg cgatgatcga gcaatcggct ctcgctgatg tctatcgccg gctggcggcc
     2761 cgcctcggat cgagtcccga gctcaccgat ctcgacagag tagtcgtcgc cctcggggcg
     2821 accatgaagc gccttgaaac cgctctcatg cagactcggt tgcttccgct ctcgaccgtc
     2881 ttcgggcgat tcacccgctc ggtgcgcgac ctggctcggg accagcgcaa gaaagtcaga
     2941 ctggtcattg agggcggcga caccctgctc gacaagacca tcctggaccg gctgggagag
     3001 ccgctggtac acctggtgtc gaatgcggtc gtccatgggg tggagtcgga atccgaacgc
     3061 gcccgcgtgg gcaaggcgcc cgaggctacc gtaggacttc gcgccgtcgc tctcgccgat
     3121 cgcgtgtcga tcacggtgtc ggatgacgga cgcggactcg acgccacccg gattctcgcc
     3181 aaggcacgtg agctcggatt ggaagcgccc tcagcggcca ccgccgacgt ctacagcctc
     3241 atcttcgtgc ccggcttctc gaccgccgag cgggtgtctc acatcgccgg ccggggcgta
     3301 gggctggatg cagccgcggc ggcgattcac gacctgggcg gcagcataga ggtcgcgagc
     3361 cgaccggggc agggaaccgt gttcacgctg agtctcccgc ttaccctggc gatcgtgcgt
     3421 tcgctaatcg tggaagtaga tcgcgagcgc tatgcggttc ccctgagcca tgtggccgaa
     3481 accgttcggc tcgaggccgg cacacttcac gaaatcaacc gccagggcgt tctgatgtgg
     3541 cgcggaagcg cgattcacgt agccgacggg ggtcggctcc tgggaaccgg ttcgagccgc
     3601 tcagcgccgc gaaactactt cgtggtgctg tttgccggcc cgcgccgccg gggccttctg
     3661 gtggaccggc tggtcgggca tcaggacgtc gtcgtgaaga gtctcgatcc gagccttggc
     3721 cgtcccgatg tcgtgtcggg cgccacgatc cttggcgacg ggagggttgc ctgcattctg
     3781 gacgctgccg gtatcgtcgc acagcgggag gcggcctgac atggctcgca acagcgcaaa
     3841 gttctcggtc cctgtcgctc ctccgcccag cccaccgccg ggcccaaccg acgtattcca
     3901 gtttgctgat cagctggccg ccgatgcctc gaacattcag gcgccgaggc cggtaaccac
     3961 tcttcacctg atcacgtttc gtctcgacgg ggaggagttc ggcctcccgg tcgagaccgt
     4021 tcgcgaggtg atccgagtgg gcgagatcac ccgggtgccg cagtcgcccc ctcacatccg
     4081 cggtgtaacc aacctccgcg gtaggatcct gccggtcgtc gagatccgca gccgcctcgg
     4141 gctcgctccg ctggtgcccc gctccaccgc ccggatcatc gtggctgaag tccgtggccg
     4201 cgtgctcggc ctgctggtcg actcggtcgc tcaggtccag aaggtgccgg ccgagcgggt
     4261 ggtcgcgacg ccggacgagg tcaagtcagg acattcggat tacctgatcg gtgtggttca
     4321 gctgtacgaa cggttgctca ttctcctcga tctccctcgg gcgctggaag cgaccgggga
     4381 ctcccactcg tgagcgcgcc atgactcaga cgatcatgca gaagattcgc gactggacga
     4441 tccgccggaa gattctgacc ggcttcgctg cagtcctcct gctcacgagc attcaagggt
     4501 ggttcgcgat tcgccagctc gagcggctgg ccgatacgag cggcgcggct ctgcaagcct
     4561 tcggcaacac ccgctcgatc attctgctca ccaccatcgt cacggtcgcc gtggggctgg
     4621 cgctcgcgat gttgctctcc cggcttatcg cgaatccgct cgaaagcctc ggcatcctgg
     4681 cggaggaggt ctcgaaggga aacctggccg cggatatacg cagccaatcc cgtgacgaga
     4741 tcgggtggct ggagcactcg atgcgtcaga tggtgaagaa tctgcgcctc gtggtagggc
     4801 agatctccac ctcgtcccgc acggtcgcaa gctccgccga ggagatctct gcctcttcgg
     4861 tacagatggc gaaaggtgcg gaggcgcaga ctaactcgac cgaggaaacc tcggcgacca
     4921 tggtagagat ggcggcccag atgcaggcgc tggcccgcaa cgccgaggcg ctggcagcga
     4981 gcgtggacga gacgtcgtcg tccattcagc agatgagcgc cacgctggtg cagaccgccc
     5041 agaacggcga gaccctgctt cagtcggtcg acgaggcaat gtcgcggctc aatggtatga
     5101 tcgagaacat cggggcgatc gcctcacggg tgcacatggt ggacgaggtc tcccagcgct
     5161 cggtgaccga ggccaagagc aatggcgagc agctccaggt ctccatcaac tccatcagta
     5221 cgcagtcgga ggagatcggg aagatcgtca aggtgatcga gggcatcgcc gatcagacca
     5281 acctcctcgc cctgaatgcc gccatcgaag cggcgcgggc cggtgaggct ggccgcggct
     5341 tcgccgtggt ggccgacgag gtccggcggc tggcagaacg ctcggtctcg gcgactcagg
     5401 agatcggggc ggtcatcgag tcggttcagc gcgagacccg tgcagccgtc gagctgatgg
     5461 gcggcgtgct cggcgccatc gtcggatcga tcgacaagac ctcccatctc gtcggtgaag
     5521 cggcgcgcgc aaccgaggag cacgcgcttg gagccaaaga agtgctgcgc atggccggca
     5581 gcatggcgag tctcacgcgg cagatcgccg cctcggtccg cgagaacgcg gtcggcgcga
     5641 gggagattac cacagccgcg cagaagatga accagctcac ccatcagatg tcggaggccg
     5701 tgtccgagca gaagcgtggc ggcgaaatgg tggtgaaggc ggtggagtcg gtcgccctgg
     5761 tgtcacgtca gaatctggtt gcggtggagc agatgagtgt cgccgccaag acgctggcag
     5821 gcgaatccga gctgctcaag cagcgggtcg aaaccttcca gctgccggcg gcgtgagctc
     5881 acccatgtcc cgggccggag ctgccgaacg gcttgccgcg ctcactgcgg cgactgggga
     5941 agagcgtcag gcgctcgtgc tcgctgcatt cgaggacgag gctccgtcgg ttcgggaaca
     6001 ggccattcga ctcgccacgc ggtacgtcga gcccgaaatt ctcggagggc tggtcgctga
     6061 cggggtgaat gcatcgcgcc ggaatggcgc gctggctgcg ctcgaacgcc agggcccgta
     6121 cgcggtcccc tatttggtga agctgctgag ttcgcaggac ggcgagctgg tcatgttcgc
     6181 gcttcagatt ctggcgcgga tcggcgacgc tgccgcggcc gggggcatgc tgccgctgct
     6241 ccaccatgcc gatcccaatg tggcgcaggc cgcagtcgag gcgctgggcc gtttgcgtgc
     6301 caccggggcg gtaccggcgc tgctcgagca gctccgcggc aacctctggc tccagctggc
     6361 tgcggtgaac gcactgggag agatcggcga tccggcagcc gtacctgctc tcctgagcct
     6421 ggttccggac agcttcgttg ccgagcaggc ggtgcggtcg ctgcagcgga tcgccgcgcc
     6481 tgattcgctg gagccactgc tcagcgtgct cgcgtcggtc cacgagcgac cgctccgcga
     6541 cgcgattctc gaagccctcg gcgtggtgct cgacctgcac cc
//
LOCUS       DASRJQ010000213         3907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3808729, whole genome shotgun sequence.
ACCESSION   DASRJQ010000213 DASRJQ010000000
VERSION     DASRJQ010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3808729"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2305
                     /locus_tag="VFB89_04795"
     CDS             <1..2305
                     /locus_tag="VFB89_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZM26649.1"
                     /translation="LVYLRPGANALDVKKAMVARMNELARTFPQGVRWSIVFDTTPFI
                     TESVKEVVTTLFEAMLLVTLVVFVFLQSWRATLIPVLAVPVSIIGTFFGLELLGFSIN
                     LLTLFGLVLAIGIVVDDAIVVIENVDRIMEEEHVPTAVAADRAIRQVAGALIAIVLVL
                     CSVFIPVAFLGGITGLMYKQFAITIVVSVVLSGIVALTLTPALCALLLKPEHAGRRPR
                     FFNWFNRGFERLTQRYIGGVGGVLTRPARFLACFAVMIVLMFVVFRRVPTAFIPTEDK
                     GYFAAIVQLPDGASRQRTDEVVGRLERFLGQQPAVKNVVSLIGLNFLLGANQTNSATI
                     FVLLQPWDERKGAANQLDAVLQQTNGFLFSMKDAQAFAFNLPEIPGLGSTAGLEMNLQ
                     DRGVNDIRRFARLAGDFTQQANALPEVGGVQPQIRVNVPQIFVDVDRAKTKALGVSLT
                     DLFQTLQALLSTLYINDFNLYGKTYRVQAEAQAQFREKPEDIGRFYVKGADTAMIPVS
                     TLVRTRFQGGPSLVTRFNGFTSALVTGAPKPGYSSGQMLNAIDQLMVSQFIPQGVGYA
                     LSGQSFQERASTGQGRIVFILGIVMVFLVLAAQYESWSIPFAVMLGVPFGIFGALLGV
                     WLRGMPNDIYFQIGLVVVVGLAAKNAILIVEFANELRAQGLSIRDSAIQAGRERLRPI
                     LMTSFAFILGVAPLVTASGAGAASRHSIGTGVFFGMLFATTIGVFFIPLFFEVIRGLS
                     ERRLFSQPAPEPAPAGAGSSPTRDQEL"
     gene            2308..3789
                     /locus_tag="VFB89_04800"
     CDS             2308..3789
                     /locus_tag="VFB89_04800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZM26650.1"
                     /translation="MRRALLAALAAGLAGACAIGPSYHRPDVGTPSTYRPPSATADSL
                     RPFYDSLRASRESTVAEPTIAIDSIANLDWFDLLQDPELRKLVETAIAQNRDVRVALA
                     TISEFRAQYGATRGALFPRVDLNAFGGTQKAVFGSLPIASTFDVLSATADLQWELDFW
                     GRIRRSTEAAKSDLLSREENQRAVILTLVSDVATAYLELRELDVDLEISKRTLASNEE
                     TLRLARRRFEQGLISELDVRQFESEVASPAARVADFERQIVQKEDQISVLVGRNPGPI
                     PRGRALTETLAPLTIPAGLPASLLDRRPDVRQAEEDLHAAMARIGVAKGELMPKFTLT
                     GEYGTQSQDVNDLFHNSTEIYQLFGGVSMPLFTGGQVGKQVDVARARTEQARYRYEQT
                     ILLALQDVNDALVGVRATRDVTAAQERQTGALRAAYRLADERYQNGISNYLDVLDAQR
                     NLFSAELSLASAQRQQLVAAVQLYKALGGGWPRAIGADSTRAQ"
BASE COUNT          657 a         1205 c         1250 g          795 t
ORIGIN      
        1 cctggtctac ctgcgcccgg gcgccaatgc gctggacgtg aagaaggcga tggtcgcgcg
       61 gatgaacgag ctggcgcgga cgtttcccca gggagtgcgc tggagcatag tcttcgatac
      121 gactcccttc atcaccgagt cggtcaagga agtcgtcaca acgctcttcg aggcgatgct
      181 gctggtcacg ctggtggtct tcgtattcct tcagagctgg cgggccacgc tgattccggt
      241 gctcgctgtg ccggtctcga ttatcgggac gttcttcggg ctcgagctcc tcggcttctc
      301 catcaatctg ctcaccctgt tcgggctggt actggcgatc ggcatcgtgg tggacgacgc
      361 aatcgtcgtg atcgagaacg tggaccgaat catggaggag gagcacgttc ccaccgcggt
      421 tgcggccgac cgcgccatcc ggcaagtcgc cggggctctg atcgcgatcg tgctggtgct
      481 ttgctcggtt ttcatcccag tagccttttt gggcgggatc acgggcctga tgtacaagca
      541 gttcgccatc acgatcgtgg tgtcggtggt cttgtcgggc atcgtggccc tcaccctcac
      601 gccggccctc tgcgccttac tgctcaagcc ggagcacgct ggacgccgac cacgcttttt
      661 caactggttc aaccgcggtt tcgagcgact tacccagcgg tatatcggcg gcgtaggggg
      721 ggtgctcacc cgcccggcca gattcctggc ctgtttcgcc gtgatgattg tgctgatgtt
      781 cgtcgtattc cggcgggtgc cgaccgcttt tatcccgact gaggacaagg gatactttgc
      841 cgccatcgtg cagcttcccg acggggcgtc gcgacagcgg acggacgagg tggtcggccg
      901 tctcgagcgg ttcctggggc agcagccagc cgtcaagaac gtggtgtcgt tgatcgggct
      961 gaacttcctc ttgggcgcca accagaccaa ctcggcaacc atttttgtgc tgctacagcc
     1021 atgggacgag cggaagggag ctgccaacca gctcgatgcc gtgcttcagc agaccaatgg
     1081 cttccttttc tcaatgaagg acgctcaggc attcgccttc aacttgccgg agatccccgg
     1141 gctcggctcg accgccggtc tggagatgaa cctgcaggat cgcggggtta acgacattcg
     1201 ccgttttgcc cggctggcgg gcgacttcac ccagcaggcg aatgctctgc ccgaagtagg
     1261 tggggtccaa ccgcagatcc gggtgaacgt tccccagatc ttcgtcgacg tggatcgggc
     1321 caagaccaag gcactcggtg tttcgctcac agacctcttc cagaccctgc aggcgcttct
     1381 ctcgacgctt tacatcaatg acttcaacct gtacggcaaa acgtaccggg ttcaggccga
     1441 ggcccaggct cagttcaggg aaaagccgga ggatatcggg cgtttttatg taaagggcgc
     1501 agacactgca atgatcccag tttctacgct ggtccgcacc cggttccagg gtggtccaag
     1561 tctggtgacc cgtttcaacg gctttacctc cgcgctggtt acgggagccc ccaagcccgg
     1621 ctacagctcc ggacaaatgc tcaacgccat cgatcagctc atggtgagcc agttcattcc
     1681 gcaaggagtg ggatatgcac tcagcggaca atccttccag gagcgggcct cgaccgggca
     1741 gggccgaatc gtcttcatac ttggcatcgt aatggtcttc ctggtgctgg cggcgcagta
     1801 cgagagctgg tcgatcccat tcgccgtgat gctgggagtg cctttcggca ttttcggcgc
     1861 gctgctggga gtgtggcttc ggggcatgcc gaacgacatc tacttccaga tcggcctggt
     1921 cgtcgtggtc ggcctcgccg ccaagaacgc cattctgatc gtcgaattcg ccaacgagct
     1981 tcgggcgcag gggctttcca ttcgtgactc ggccatccag gccggtcggg agcgactccg
     2041 cccgattctg atgacgtcat ttgccttcat cctgggtgtc gcaccgctgg tgacggcgtc
     2101 cggggccggc gccgcgagcc gacattcgat cgggacaggc gtcttcttcg gaatgctgtt
     2161 cgccacgacc atcggtgtct ttttcattcc tctgttcttc gaggtaatcc gcgggctgag
     2221 cgagcggcga ctgttctcgc agcccgctcc ggaaccggcg ccggccggcg ccggttcatc
     2281 tcccacacgc gaccaggagc tgtgaccttg cggcgtgctc tccttgcggc gctggccgcc
     2341 gggctggccg gcgcgtgcgc cattggccct tcgtatcatc ggccggatgt cggcacaccc
     2401 agcacatatc ggcccccttc agccacggcc gattcgttga gacccttcta cgactcgttg
     2461 cgagccagcc gcgagtccac tgttgccgag ccaaccatcg cgatcgattc gatcgccaac
     2521 ctcgattggt tcgacctgct gcaggacccc gagctgcgca aactggtcga gactgccatt
     2581 gcccagaatc gcgatgtgcg ggtcgcgctg gcgacgatct ccgagttccg ggcgcagtac
     2641 ggcgcgacca gaggagccct gtttccccgg gtcgatctga acgcgttcgg ggggacgcaa
     2701 aaggcggtat tcggatcctt acctatcgct tccacgttcg acgttctgag cgccacggcc
     2761 gacctccaat gggagctcga cttctggggg cgcattcgcc gatccaccga ggcggcgaag
     2821 tcggacctgc tttcgcggga agagaaccag cgagcggtga ttctgacgct ggtgagcgac
     2881 gtcgcgacgg cgtacctcga gctgcgcgag ctggacgtgg atttggagat ctccaagcga
     2941 acgctcgctt ccaacgagga gacgctccgg ctggctcgcc gacgcttcga gcaaggcctg
     3001 atctctgagc tcgatgtgcg acagttcgaa tcggaagtgg cgagcccggc tgcgcgggtg
     3061 gctgatttcg agcggcagat cgtgcagaag gaagaccaaa tcagtgtcct ggtgggccgg
     3121 aatccgggtc ccatccctcg cgggcgagcg ctcaccgaaa ccctggctcc gctcacgatc
     3181 ccggctgggc ttccggcttc cttgctcgat cgccggccgg acgtacggca ggcggaggag
     3241 gacctgcacg cggccatggc tcgcattggt gtggcgaagg gcgagttgat gcccaagttc
     3301 acgctgacgg gggagtacgg cacgcagagc caggacgtga acgatctctt ccataattcg
     3361 accgagatct atcagctgtt cggcggggtg tcgatgccgc tgtttaccgg cgggcaggtg
     3421 ggcaaacagg tggacgtggc acgtgcgcga accgaacagg ctcgctatcg ctacgaacaa
     3481 accatactgc tcgcgctcca ggatgtgaac gacgccctgg tgggtgtacg cgccacgcgc
     3541 gatgtgacgg cagcccagga acgccagaca ggggcgcttc gggcggcgta ccggctggcc
     3601 gatgaacgct atcagaatgg gatctcgaac tacctcgatg tgctggacgc acagcggaat
     3661 ctgttctctg ccgagctctc gctggcgagc gcgcagcgcc agcagctggt cgccgcggtt
     3721 cagttgtaca aggcgcttgg aggagggtgg cccagagcga tcggcgccga ttcgacgcgg
     3781 gctcagtaac gggtgtatct gccttgaggc tgtgctatcg gtcggctact ttcacagcgg
     3841 ctcgccactc gggcgggccg ccttcccgag tagctcagtt ggtagagcag ctgactgtta
     3901 atcagcg
//
LOCUS       DASRJQ010000214         4431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_143939, whole genome shotgun sequence.
ACCESSION   DASRJQ010000214 DASRJQ010000000
VERSION     DASRJQ010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143939"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..893
                     /locus_tag="VFB89_04805"
     CDS             117..893
                     /locus_tag="VFB89_04805"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZM26651.1"
                     /translation="MTSAPVADDPERLQARIETLEQEKRHLLAVVEILQEIAGSLHFV
                     DVVQAVTRRLGEAFGLDRCSIFLAERGGETVRLVASYEDPSIRNYVVDLARYPELRRA
                     LETGQIVFIPEVVGDPGLRHAQDALASRRVKNITVIPITWRSAPIGAIFLRTFRDGPA
                     FTEADVRFCQVVASLTAKALRNAHRFERLQLRRGEDPAGRAADRERAALVGFLRRLLD
                     AFAAKEEPWGEGALARSSTAEVDRLVGVAMTVLSQEAAAR"
     gene            890..2911
                     /gene="ligA"
                     /locus_tag="VFB89_04810"
     CDS             890..2911
                     /gene="ligA"
                     /locus_tag="VFB89_04810"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HZM26652.1"
                     /translation="MTVGASTELAARVEALRSQLERANHAYYTLDAPEITDAEYDRLF
                     RELQSLEAEHPELLTPDSPTQRVGAPPASALAKYRHRRPMLSLANAFSAEELAAWEQR
                     NARLVAEVVSAGYTTEVKIDGAAVSLTYERGRLSMGSTRGNGVIGEDITANLKTIPDV
                     PLVLKGSGHPAVIEVRGEVYMPYDSFVRVNRQRDKAGEPPLANPRNAAAGGLRQLDPN
                     LTRQRRLRMFAFQIETIEGRLGAKTHQEELDLLEQWGFQVEKHRQRHGDLAAVERRAE
                     ELESVLATLPFQADGVVVKVDRLSLHPELGVVGEREPRWAIARKFAPEVAQTRLKAIH
                     VNVGRTGALNPWAELEPVELGGVVVSRATLHNEDLIAQKDIRIGDWVEVVRAGEVIPQ
                     LLGPLRDRRTGDEQPFAMPDTCPVCGTPVERPTDEAMRYCPNISCPGRVLESIVHFAS
                     RGAMDIRGLGYERVRQLLQEGLIHDVADLYDLDVDRLVRLERFAEQSASQLVQAIAAS
                     KERPLSLLLFGLGIRHVGYTVAQLLARRFGSMDALMKATEAGINDVPGIGSAIAEAVV
                     HFFGESRNRELIERLRRAGLQFTEPTAVAEGGPLAGKTYVLTGTLPTLSRADATQLIE
                     RAGGRVIGSVSKKTDAVVAGEDPGSKLEKAKALGVEVIGEEELVRRAGG"
     gene            3049..3630
                     /locus_tag="VFB89_04815"
     CDS             3049..3630
                     /locus_tag="VFB89_04815"
                     /inference="COORDINATES: protein motif:HMM:NF014845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V4R domain-containing protein"
                     /protein_id="HZM26653.1"
                     /translation="MATTPTFKPGRYGVTLGPRVLQQLRTSLERDAGLQAAGYLQEAG
                     FAGGEALYSAYAAWLGENYGIERPGDLDAQFLGEALWHFFAELGWGSVTVTPLGAAAM
                     ALDSPDWAEALGDAKGEYPSCHLTCGLLADFLGRISDGLVAVMEVECRSRGDARCRFL
                     AGSPETLSTLYERMAQGMSYGEALGIAGGGNPA"
     gene            3751..>4431
                     /locus_tag="VFB89_04820"
     CDS             3751..>4431
                     /locus_tag="VFB89_04820"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZM26654.1"
                     /translation="MPELLGQLEHALGTAYRLERELGGGGMSRVYLAQELALGRQVVI
                     KVLPPDLAAAINVERFRREIQLAAKLQHPHVVPLLAAGQAGDLLYYTMPFIEGESLRS
                     QLSRQGELPVTEAVRYLRDVVDALAYAHEQGVLHRDIKPDNVMLSRHHALVTDFGVAK
                     ALSEAADQAGASGFTSAGVAMGTPAYMAPEQAAADPTIDHRADIYSVGAMAYEMLTGH
                     PPFSGRTPQ"
BASE COUNT          774 a         1417 c         1496 g          744 t
ORIGIN      
        1 tctcgacatc tcgagaattt gcaaaggcaa ctgaagaagc caaacacggt tccgctgccc
       61 ccgggagaac ccttataatc gtacggtaat tctcacctct tccaacggtg gtcctgatga
      121 cctcagcccc ggtcgccgac gaccctgagc gcctgcaagc gcgaatcgag acactggagc
      181 aggagaagcg gcatctcttg gcggtggtcg agatcctcca ggagatcgcg ggctcgctcc
      241 acttcgtaga tgtggttcag gcggtcaccc gtcgattggg cgaggcgttc ggattggacc
      301 gctgctcgat cttcctggcc gaacgaggcg gcgaaacggt tcggctggtg gcaagctacg
      361 aagaccccag catccggaat tacgtggtgg acctggctcg ctatcccgag ctgaggcgcg
      421 cgctggaaac cggacagatc gtgttcattc ccgaagtggt cggcgatcca gggctgcgcc
      481 acgcgcagga tgccctcgcg tcccgacggg tgaagaacat caccgtcatt cccatcacct
      541 ggaggagcgc gccgatcggg gcgattttct tgcgcacctt ccgcgacgga ccggcattca
      601 ccgaggccga cgtgcgcttc tgtcaggtag tcgcgagtct cacagcgaag gcattgcgaa
      661 acgcccaccg cttcgagcgg cttcagctcc gtcggggaga ggacccggcg gggcgggctg
      721 cggaccgcga acgggcggct ctggtgggat tcctgcggcg cctgctcgac gcgttcgctg
      781 cgaaggaaga accctggggc gagggagcgc tggcgcgctc cagcacggcc gaggtggata
      841 ggctggtcgg cgtcgccatg accgttctgt cccaggaggc cgcggcgcgg tgaccgtcgg
      901 cgcctccacc gagctcgccg cgcgagtgga ggcgctccgc agccagctcg aacgggccaa
      961 tcatgcgtac tacacgctcg acgcgccgga gattaccgac gccgagtacg atcgcctctt
     1021 tcgtgagctg cagtcgctcg aagcggagca tcctgagctt ctcacccccg acagtcccac
     1081 ccagcgcgtc ggcgctccgc cggcaagcgc gctcgccaag taccgtcatc gccgcccgat
     1141 gctgtcgctg gccaacgcgt tctcagccga ggagctggcc gcctgggaac agcgaaacgc
     1201 gcgactcgtc gccgaggtcg tgagcgcggg ttacaccacc gaagtgaaga tcgatggggc
     1261 ggccgtgagc ctcacttacg agcgcggtcg cctctcgatg ggttcgacgc ggggcaacgg
     1321 cgtgatcggc gaggacatca ccgccaatct caagaccatc ccggatgtcc cgctggtact
     1381 caaaggatcg ggacatccgg ccgtgatcga ggttcgcggc gaggtgtaca tgccgtacga
     1441 ctccttcgtt cgggtaaacc gccagcgtga caaggccggt gaaccgccgc tggcgaaccc
     1501 acgcaatgcg gcagccgggg ggttgcgcca actcgacccc aacctcaccc ggcagcgccg
     1561 gctgcggatg ttcgcattcc agatcgagac catcgagggc aggctcggtg cgaagacgca
     1621 ccaggaagag ctcgacctgc tcgagcaatg gggctttcag gtagagaagc accggcagcg
     1681 gcatggcgat ctcgcagcag tggagcggcg cgcggaggag ctcgagagcg tgctggcgac
     1741 gttgccgttc caggcagacg gggtggtggt gaaggtcgat cgcctgtcgc tgcaccccga
     1801 gctcggtgtg gtcggcgagc gggagccccg ctgggcgata gcgcggaagt tcgcgcccga
     1861 ggtggcacag acccggctca aggcgatcca tgtcaacgtg gggaggaccg gcgcgctcaa
     1921 tccgtgggcc gagctcgagc cggttgagct gggcggcgtc gtggtctcga gagcgacgct
     1981 acacaacgag gatctcatcg cccagaagga catccgaatc ggcgactggg tcgaggtggt
     2041 tcgggccggc gaagtcattc cgcagctcct cggtccgctg cgggaccggc gcaccgggga
     2101 cgaacagcct ttcgccatgc ccgacacctg tcccgtgtgc ggcacgccgg tcgagcggcc
     2161 gaccgacgag gcgatgcgct actgtcccaa catctcgtgc cccggtcgcg tgctcgagag
     2221 catcgtccat tttgcgtccc gcggcgcaat ggacatccgc gggctgggat acgagcgggt
     2281 ccgccagctt ctccaggaag gcctcattca cgacgtggcg gatctttacg acctggatgt
     2341 cgaccggctg gtccggctcg agcggttcgc cgagcaatca gcatcgcagc tggtccaggc
     2401 catcgcagcc tcgaaagagc gccccctttc cctgctcctt ttcggtctcg gcatccggca
     2461 cgtgggctat acggtggcgc agttgctggc ccggaggttc ggctcgatgg atgcgctgat
     2521 gaaggcgaca gaggccggga tcaacgacgt tccgggcatc ggcagcgcca tcgccgaggc
     2581 cgtggtccac ttcttcggcg agagccgcaa ccgggagctg atcgagcgac tgcggcgggc
     2641 gggtcttcag ttcaccgagc ctaccgcggt cgccgagggc ggcccgctgg cagggaagac
     2701 ctacgtgctg acaggcacgc tgcccacgct ctcccgggcc gatgcaaccc agctgatcga
     2761 gcgagccggc ggccgggtga tcggctcagt gagcaagaag acggatgcgg tggtagctgg
     2821 tgaggacccg ggcagcaagc tggagaaagc gaaggcgctc ggagtggagg tgattgggga
     2881 ggaggaattg gtgagacggg cggggggcta ggagagctat cagctatcag ctatcagcta
     2941 tcagctatca gctatcagct atcagtccgc ttggataatc gcttgggtct cttaacttca
     3001 aggggcctct gatagctgat agccgattag ctcacattag gaattccaat ggccaccacc
     3061 ccaacgttca aacctggccg gtacggcgtc accctcggcc cgcgcgtgct acagcagctg
     3121 cgcacctccc tcgagcggga tgccggcctg caagccgccg gctatctcca ggaagccgga
     3181 ttcgccggcg gtgaggcgct ttattctgct tacgccgcgt ggctgggtga gaactacggg
     3241 atcgagcgcc cgggcgacct ggatgcccag tttctgggcg aggcactctg gcacttcttc
     3301 gcggagctcg ggtggggcag cgtcaccgtc acaccgctcg gcgctgccgc gatggcgctc
     3361 gattcgcccg attgggccga ggcgttggga gatgccaagg gggagtatcc atcctgccat
     3421 cttacctgcg gcctgctggc cgactttctg ggtcgaatct ccgatgggct ggttgccgta
     3481 atggaggtcg aatgcagatc gcggggcgat gcccggtgcc gctttctggc aggatcgccc
     3541 gaaacactga gcacgttata cgagcggatg gcgcaaggga tgagctacgg tgaggcactc
     3601 ggaatcgcag gcggcggcaa cccggcctga cccgcctcgc gacgatttcg agggcccggg
     3661 catccccggg ccctcgtcgc ttttggctcg gcgcgctggc cccaatccat tgaggtacct
     3721 atactttccc cattccccca gggaacggtg atgccggagc ttctcgggca actggagcac
     3781 gcgctcggca ccgcctaccg cttggagcga gagctcggcg gaggcggaat gagccgggtg
     3841 tacctcgccc aggagctggc gctgggacgc caagtcgtca tcaaggtgct gccgccggac
     3901 ttggccgccg ccattaacgt cgagcggttc cggcgcgaaa tccagctggc tgccaagcta
     3961 cagcatccgc atgtcgtccc actgctcgcg gcggggcaag ccggcgacct gctttactac
     4021 acgatgccct ttatcgaggg cgaatcgctt cggtctcagc tctcccgcca gggcgagctg
     4081 ccggtaactg aagccgttcg atacctcagg gacgtggtcg acgcgttggc ctacgctcac
     4141 gagcaaggcg ttctccatcg cgacatcaaa cccgacaacg tgatgctcag ccggcaccat
     4201 gcactggtga ccgacttcgg cgtggccaag gccctgagcg aggccgcgga tcaggccggc
     4261 gcgagcggat tcacttcggc cggtgtcgca atgggcaccc cggcctacat ggcgccggag
     4321 caggccgcgg cagatccaac gatcgaccat cgggcggaca tctactcggt cggcgctatg
     4381 gcgtatgaaa tgctcaccgg acaccctccg ttctccggtc gcacgccgca g
//
LOCUS       DASRJQ010000215         3318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3806260, whole genome shotgun sequence.
ACCESSION   DASRJQ010000215 DASRJQ010000000
VERSION     DASRJQ010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3806260"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VFB89_04825"
     CDS             <1..804
                     /locus_tag="VFB89_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26655.1"
                     /translation="RVEDFSSYTSDENYRANPHGWQIPSPRWFNQGKLHLDPTVRYEG
                     HQTLRYDWSTPPTPTAWCMKDITRGAGYKAPDVPEMWIEIAHKFAPTFDTNKRGMGGA
                     CGVAEYKFLLFWLKTPQGGHRFDVTSGMNGNQWWSAHPGGANQTAYGTNCSGVGFKCR
                     LGYGDGQERFRPLVPKTPLYDGQWHVYRVHIKLPTVQGDATGVFEFWLDGVLLKQVTG
                     QTFISSKGVWSNRLDFIALGANSNSGTSQATSNWWGHLKIWTSKPSWAE"
     gene            complement(819..1508)
                     /locus_tag="VFB89_04830"
     CDS             complement(819..1508)
                     /locus_tag="VFB89_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZM26656.1"
                     /translation="MSNSPIRILLIDDHALFRESVARLLGGEPGFHVVGGCASVHAAL
                     ELLRQEAVDIVLLDFDLGGRNGTQFMHAARERGFGGQVLVVTAGVDEEEAADLIQAGV
                     SGIFMKHKSAPLLAQAIRDVTAGKVWFDPALLRAAMAHGPRSSADAQERRFTLRERQV
                     FSYVLEGLANKEIGGRLGVSESAVKATLQQLFSKTGVRTRSQLVRIALEQYPDLATDA
                     PSRLGPSESGG"
     gene            complement(1501..2904)
                     /locus_tag="VFB89_04835"
     CDS             complement(1501..2904)
                     /locus_tag="VFB89_04835"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HZM26657.1"
                     /translation="MKTRVPFPPIVSRRTSLALAGLLVIAIAVTDWLTEPYISIGFLY
                     LFPIMIAGACLSRTQTIVIALGCAVLQEAFSNLPESEMIARLMFSSVGFVSTGLLVGE
                     LLRNRRRALAHALELEAQAKLRQDAEEQVRILVESSPAAIVVIDAVGRILLANEAANQ
                     VLTPGGIPLPGQCIGDYLPPLQAAVRNHPSRSLRAAMQCRGQRANHEPFLAGVWFSTY
                     ETIAGPRLAAIIVDLSEDLRSREDLRFDHLLKHARIAMSALAHEIRNLTSAVQVVHNN
                     LLRVKELEQNEDFRALSTLVRSLENIAALELPPARPDKTEAMELTPVLDELRVLLDAA
                     YRESDIDLQWQIPESLPLVRAHPYGLVQVFLNLAKNSQRAMQTTQVKRLTVSAAVEAH
                     SVVIRIEDTGVGVASPEDLFRPFQRNAAAAGLGLYVSRAMMRSFGGELLYETGSKGCR
                     FAMVLPVSSTVGETVSV"
     gene            3073..>3318
                     /locus_tag="VFB89_04840"
     CDS             3073..>3318
                     /locus_tag="VFB89_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26658.1"
                     /translation="MRWFLMFLEVAASMVPACATWPLGERLDSGASGLAGEWIGPPAP
                     GSTDTVVWRFRDDGTYERLRAATTHDPGEPGMPVPVAE"
BASE COUNT          646 a         1069 c         1029 g          574 t
ORIGIN      
        1 cgggttgaag atttctccag ctacacgagc gacgagaact accgcgccaa ccctcacggc
       61 tggcagatcc cgtcgccccg ctggttcaat cagggcaagc ttcacctcga cccgaccgtc
      121 aggtacgaag ggcaccaaac ccttcggtac gactggagca caccaccgac gccgactgcc
      181 tggtgcatga aggacattac gcgcggggcg ggatacaagg cacccgacgt tcccgagatg
      241 tggatcgaga ttgcgcacaa gttcgccccg accttcgaca ccaacaaacg cggtatgggc
      301 ggcgcgtgtg gcgtcgccga atacaaattc cttctgttct ggctcaagac tccccagggc
      361 ggtcaccgtt tcgacgtgac tagcgggatg aacggcaacc aatggtggag tgcacacccc
      421 ggaggcgcga accagacggc ctatgggacg aattgctcag gcgtaggctt caagtgccgg
      481 ctgggctacg gcgacgggca ggagcgtttc cgaccgctcg tgcccaagac gccgctctac
      541 gatgggcagt ggcacgtcta ccgggtccac atcaagctcc ctaccgtcca gggcgacgcg
      601 accggcgtgt tcgagttctg gctggatggc gtgctcctaa aacaggtcac cggccagacc
      661 ttcatttcgt cgaagggtgt ctggagcaat cggctcgatt tcatcgcgct gggggcgaac
      721 agcaacagcg gcacttccca ggcaaccagt aactggtggg gccacttgaa aatctggacc
      781 tcgaaaccat cctgggcaga gtgacgtgac ccggagtcct acccaccgct ctcactgggc
      841 cccaaccgag aaggcgcgtc cgtggccagg tcggggtact gttcgagagc aatgcgcacc
      901 agctgactgc gagtccgcac ccccgtcttc gagaagagtt gctgcagcgt tgccttgacc
      961 gcgctctcgg atacgccaag gcgcccgccg atctccttgt ttgccagccc ctcgagcacg
     1021 taggaaaaga cctgacgctc ccgcaaggtg aaccgtcgtt cttgagcatc cgccgaagac
     1081 ctaggtccgt gcgccatcgc tgcccgcagg agcgcaggat cgaaccagac cttgcctgcg
     1141 gtaacatccc ggatcgcctg ggccagcagt ggggccgatt tgtgcttcat gaagattccg
     1201 gagacgccgg cctgaatcag gtcggccgcc tcttcctcat cgactcccgc cgtcaccacc
     1261 agcacttgcc ccccaaatcc gcgctccctc gcggcatgca tgaattgggt gccgttacgg
     1321 ccccccaggt cgaagtcgag caggacgatg tcgacggcct cctgccgaag gagctcgagc
     1381 gccgcgtgga ccgaagcaca gccaccaacg acgtgaaatc ccggttcacc tcccaacagg
     1441 cgggcgacac tctcccggaa gagagcatga tcatcgatca gcaggatgcg gatcggcgag
     1501 ttagacactg accgtctcgc cgaccgttga tgacaccggg agcaccatgg cgaagcgaca
     1561 ccccttggac cccgtctcgt acagcagctc gccgccgaag ctccgcatca ttgctcggga
     1621 gacatacaaa ccgagcccgg cggccgccgc gttgcgttga aacggtcgaa acagatcttc
     1681 gggagacgcg acgccgacgc cggtgtcctc gatgcggatc accacactat gcgcctctac
     1741 ggctgcgctc acggtcagcc gtttaacctg ggtggtctgc atcgcgcgtt gactgttttt
     1801 tgcgaggttc aggaagactt gtaccagtcc gtatggatga gcccggacaa gtgggagaga
     1861 ctccgggatt tgccactgca ggtcgatgtc ggattcccga taggccgcat cgagcagcac
     1921 acgcagctcg tcgagcaccg gcgtcaactc catcgcctcg gtcttgtccg gtcgcgcggg
     1981 tggcagctcc aacgccgcga tgttttccag gctccggacc agggtactga gagctcgaaa
     2041 gtcctcgttc tgctccagtt ccttcactcg caagaggttg ttgtggacga cttgtacggc
     2101 gctggtcagg ttgcggattt cgtgagccag ggcactcatg gcgatcctgg cgtgcttcag
     2161 caggtggtcg aacctcaggt cctcccggct ccgcagatcc tcggaaagat ccacgatgat
     2221 cgcggccaac cggggacccg caatggtttc gtaggtcgag aaccacacgc ccgccagaaa
     2281 gggctcatgg ttggcgcgct gcccccggca ttgcatggca gcccgaagag atcgagaggg
     2341 atgattccgg acggctgctt gcagcggcgg gaggtaatcg ccgatgcact ggccgggcag
     2401 gggaatcccg ccgggcgtga gcacctggtt cgcggcctcg ttcgccagga gtatcctgcc
     2461 gacagcatca atgacgacaa tcgccgccgg gctgctctcc accaggatgc ggacctgctc
     2521 ctcagcgtcc tggcggagtt ttgcctgggc ctcgagctcc agcgcgtggg ccagcgccct
     2581 ccgacgattg cgcagcagct caccaaccag aaggccggta gacacgaagc ccaccgagct
     2641 gaacatcagc cgagcgatca tctcgctctc aggcaggttg ctgaaggcct cctgcagcac
     2701 ggcgcaccca agcgcgatga cgatggtttg cgtccgggat aggcaggccc ccgcaatcat
     2761 gatggggaac aggtacagga agccaatcga gatgtacggc tcggtcagcc agtcggtcac
     2821 tgcaattgcg atgacgagga gacccgcgag ggcaaggctt gtcctgcgac ttacgattgg
     2881 aggaaacggc actctggtct tcatgcagtc aatggtgcgc agtcggcgca ctgcccggca
     2941 atagctccag gcgcctctgg ccaaaagata gcccgcagta gccggaagat aggcaggggc
     3001 gtcgaaagat cggattcccc tctacatctg cagcacctaa atacccgacg tcaccgcgcg
     3061 tgcggaggcc tggtgcgttg gttcctgatg tttctcgagg tggcagcctc aatggtcccc
     3121 gcgtgcgcta cctggccgct cggggagcgt ctcgattccg gcgcgagcgg acttgccgga
     3181 gagtggatcg gtccaccggc accaggctcg accgacacgg tggtgtggcg gtttcgtgac
     3241 gacgggacgt acgagcgcct gcgagccgct acgacccacg accccggcga acccggcatg
     3301 ccggtgccgg tcgccgag
//
LOCUS       DASRJQ010000216         3956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1267664, whole genome shotgun sequence.
ACCESSION   DASRJQ010000216 DASRJQ010000000
VERSION     DASRJQ010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267664"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VFB89_04845"
     CDS             complement(<1..641)
                     /locus_tag="VFB89_04845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZM26659.1"
                     /translation="MKLTWVTLSVILIAACKKEAPQPVYETIPVERRDIIVSARATGK
                     IQPDTTVEVKTRASGEVLQLKVETGQVVKRGTLLMQIDPRLSRNAVAQSEAGLEAARV
                     QLNNAEIQKRRSDSLLAAQAITAQEHDQDVLGYANAKANLVAAQVSLENNRINLQDAD
                     VLAPITGTIIEIDVSRGQVIASATSNVGGGTTLLKMADLNLVQVSTPIDETDIG"
     gene            complement(638..1912)
                     /locus_tag="VFB89_04850"
     CDS             complement(638..1912)
                     /locus_tag="VFB89_04850"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZM26660.1"
                     /translation="MATGLTLIMAGPVRAQIPGVTLEEAIRLAEQVQPSVVQARGDVA
                     TTSALLRSAKGAYLPTLAATAIGSESYGGRARVDPTTGQIVTQNWTASGGATISSTFD
                     LFTGFQRRAQTRAARANTEAAGAGLINARFTQRLTTTNQFFDALAAAQLVTVREASVR
                     DSEEQLRAAIAKLRAGTATRADTLTSRVNLGTAQVNLATAQSALVAAQASLAQLIGRT
                     GRVAALDDSSFYQSPVIDTTALRGEALQQSPRVQQSLATARAARAEVAAAKANYWPTL
                     SLGASTGVTATQGTNVGAINNTRFALQLSWSIFDGFAREAQIVTQESNADLADARALD
                     DGRAVDAGVTTYLAQLDAARTNIAITQTSVAAGEENLRVLQARYQQGVATIVDLFTAQ
                     TALAQARVDAVNARFDYLRAKAQLEALVGHPL"
     gene            2138..3433
                     /gene="asnS"
                     /locus_tag="VFB89_04855"
     CDS             2138..3433
                     /gene="asnS"
                     /locus_tag="VFB89_04855"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HZM26661.1"
                     /translation="MSPVQIADLKNHVGATVTVQGWVLTTRSSGKIAFLVLRDGSGYL
                     QCVLSRKEVSEEIWQRFLGLAQEASVEVTGSVRAEPRSPGSYELGVTDLQVLGTSVDY
                     PIGPKEHGTQFLFEHRHLWLRSRKQVAIAKVRHEVEQAMRDFLYDGGFTLVDTPILTG
                     AIGEEAGNLFATDYFDLGKAYLAQTGQLYVEAAAAALGKVYCFGPTFRAEKSKTRRHL
                     TEFWMIEPEVAFADSDANMRLQEEFVSYVVSRTIDRRKDELKELERDLTPLERVAPPF
                     PRISYTDAVARLNALGSDIQWGADLGGDDETLLAKEYDRPVFVYNYPKQVKAFYMKEN
                     PDDPRTVLNDDCLAPEGYGEIIGGSQREDDYDRLLARIKEQHLDPEAYRWYLDLRKYG
                     TFVHSGFGLGIERTVAWICGIPHIREAIAFPRQIHRLYP"
     gene            complement(3453..>3956)
                     /locus_tag="VFB89_04860"
     CDS             complement(3453..>3956)
                     /locus_tag="VFB89_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26662.1"
                     /translation="EAIERGGWRFVVLQQGPSSLPESRVNLRDWTRRFAERIRAVGAE
                     PALYAVWPSRDRLAFFGDVNESYRLAARDVGGVLLPVGQAWLDAWSRDGGLALYGPDD
                     FHPSVEGSYLAALVIYSGLSGRSPVGLPARFALPGGSAVEITPATATLLQETAASVVG
                     TTPTPGK"
BASE COUNT          671 a         1266 c         1283 g          736 t
ORIGIN      
        1 ccgatgtcgg tttcgtcgat cggggtcgat acctgaacca ggttcaagtc agccatcttg
       61 agcaacgtcg tcccgccgcc cacgttggac gtggccgagg cgatgacctg gccgcgcgac
      121 acgtcgatct cgataatcgt cccggttatc ggcgccagta catcggcatc ctgcaagttg
      181 atgcgattgt tctccagtga tacctgcgcg gctacgagat tggccttcgc attggcatag
      241 cccagcacgt cctgatcgtg ctcctgcgct gtgatcgcct gtgcggccag caggctgtcg
      301 gagcggcgct tttggatttc ggcgttattc agctggaccc gggcggcttc cagacccgcc
      361 tcggactggg caacggcatt ccgggaaagc ctgggatcga tctgcatcag cagcgtcccg
      421 cgctttacca cctgcccggt ctccaccttg agctgcaata cctcgcctga ggcccgggtt
      481 ttcacctcca cggtggtgtc gggctgaatc tttccggtgg cacgggccga cacgatgatg
      541 tcgcgtcgtt ccaccggaat ggtttcgtag accggttgcg gcgcctcctt tttgcaggca
      601 gcgatcagaa tcacgctcag cgtcacccag gtgagcttca taggggatgc cccaccaggg
      661 cctcgagctg ggccttggcg cgcaggtagt cgaaccgggc gtttacggcg tccaccctgg
      721 cctgggccag ggcggtctgg gccgtgaaca gatcgacgat cgtcgcgacg ccctgctggt
      781 aacgggcctg aagcacgcga aggttctcct ctcccgccgc gacgctggtc tgagtgattg
      841 cgatgttggt acgcgccgcg tccagttgcg ccaggtaggt cgtcacgccg gcatctacgg
      901 cgcgaccgtc atccaaagcc cgggcatcgg cgagatcggc attgctctct tgcgtcacga
      961 tctgcgcctc tcgagcgaac ccatcgaaga tgctccagct cagttgcaag gcaaaccggg
     1021 tgttgttgat ggcaccgaca ttggtgcctt gcgtcgccgt gacgccggtc gaagcgccca
     1081 ggctgagggt gggccaatag tttgccttgg ctgccgccac ttcggcgcgc gccgcgcgag
     1141 cggtggcgag cgattgctgg actcgtggcg actgttgcaa agcctcaccc cggagtgccg
     1201 tggtatcgat caccggactt tgataaaagc tggaatcgtc cagcgccgcg acccgcccgg
     1261 ttcgcccaat cagctgagcc aggctggcct gcgcggcgac cagggcgctt tgtgccgtcg
     1321 cgagattgac ctgggcggtg cccagattga cgcgcgaagt gagggtgtcg gcgcgcgttg
     1381 cggtgccggc gcgcagcttg gcgatcgcgg cccgcagctg ctcttcgctg tctcgcacgc
     1441 tcgcctctct gacggtgacg agttgggcgg cggcgagcgc gtcgaagaac tggttagtcg
     1501 tggtgaggcg ttgggtgaat cgggcattga tgagccccgc gcccgcagcc tctgtattgg
     1561 ctctagccgc gcgggtttga gcccgccgct gaaagccggt gaacaggtcg aatgtggagg
     1621 agatcgtggc ccccccgctc gcggtccaat tctgggtgac gatctgcccg gtggtcgggt
     1681 cgactcgggc gcggccgccg tatgactctg acccgatggc ggtcgcagcg agcgtgggca
     1741 ggtaggcgcc cttggcactt cgaagcagtg cggacgtcgt ggcgacgtcc cctcgtgcct
     1801 gaactaccga aggttgcacc tgttccgcca ggcggatcgc ttcctcgaga gtcacgccgg
     1861 gaatctgcgc ccggaccggg ccggccatga tcagcgtgag gcctgtcgcc acgatactgc
     1921 taccgcgccg catcagccgt ggggccggcg ggtgcatgcg tttgactgtc ggtcatacgt
     1981 agctcctgtc tcggcgaccg aaagcatccc cgggttccgt ccgcatcgct tacgcgcggc
     2041 cggtttggtg cgttgaaact cctgtttgac gctttccccg agcccgaata aactgtaccc
     2101 tcctcgtcgt cgatcccagg aacacgagcc atcttcaatg tcgccggtcc agatagccga
     2161 cctcaaaaat cacgtgggcg ccactgtcac agtccaaggc tgggtgctca cgactcgctc
     2221 cagcgggaaa atagcgttcc tggtacttcg ggacggctcc ggctacctcc agtgcgtgct
     2281 ctccaggaag gaggtctcgg aggagatctg gcaaagattt ctggggctgg cccaagaagc
     2341 cagtgtcgag gtcacgggaa gtgtgcgagc cgagccgcgc tcccccggca gttacgagct
     2401 gggtgttacc gatctccagg tcttgggaac gtcggtggat tatcccatcg gccccaagga
     2461 gcatggcacc cagttcctgt tcgagcatcg ccacctctgg ctgcggagcc ggaagcaggt
     2521 ggccattgcc aaggttaggc atgaggtcga gcaggcaatg cgcgacttcc tgtacgacgg
     2581 cggctttacg ctggtggaca cgccgattct cacgggagca atcggtgagg aggcgggcaa
     2641 tctgttcgcg accgactact tcgacctggg gaaggcgtac ctggcacaga cggggcaact
     2701 ctacgtggag gcggccgcgg ccgcgctcgg caaagtctat tgctttggcc cgacgttccg
     2761 ggccgagaag tcgaagacca ggcgacatct cactgaattc tggatgatcg agccggaggt
     2821 ggcgttcgcc gactccgatg cgaacatgcg gctccaggag gagttcgtct cctacgtcgt
     2881 ctctcgcacc atcgaccggc gaaaggacga gctgaaggag ttggagcggg acctgacgcc
     2941 gctcgagcgg gtggcgcccc cgttcccccg catctcctat accgacgcgg tcgcaaggct
     3001 caacgcgctg ggctccgaca tccagtgggg cgctgatctg ggtggcgacg acgaaaccct
     3061 gctggccaag gagtacgacc gccctgtctt cgtctacaac tatcccaagc aggtcaaggc
     3121 gttctacatg aaggaaaatc ccgacgaccc gcgaaccgtg ctgaacgatg attgcctggc
     3181 gccggaaggc tatggggaaa tcatcggcgg gtcgcagcgg gaagacgact acgaccggct
     3241 gctggcccga atcaaggagc aacacctcga tcccgaggcg taccgctggt atctcgatct
     3301 ccgaaagtac ggcacgtttg tccactccgg attcgggctt ggcatcgagc ggaccgtggc
     3361 gtggatctgc ggcattcccc acatccgcga ggcgatcgcc tttccgcggc agatccaccg
     3421 gctgtatccc tgaaggtcgc tcgagccggt tctcacttgc caggcgtcgg cgtcgtgcca
     3481 acaacggagg cggctgtctc ctgcaggagc gtcgccgtcg cgggggtgat ttcgactgcg
     3541 cttccgcctg gcaacgcgaa tcgcgcgggc agtcccaccg gcgaacggcc cgacagcccc
     3601 gaatagatca cgagcgctgc gaggtaggaa ccctcgaccg acggatggaa atcgtccggt
     3661 ccgtacaggg cgaggccgcc atcgcggctc caggcatcga gccaggcctg tcccaccggc
     3721 agcagcactc ctccgacatc ccgagcagcc agtcggtagg attcgttgac gtcgccgaag
     3781 aaagccaggc gatccctgga cggccacacg gcatacagcg ccggctcggc tcccacggca
     3841 cgaatccgct ccgcaaatcg tcgcgtccag tcgcgcaggt tgacccgact ttccggcagc
     3901 gacgacgggc cctgttgcag cacaacgaac cgccacccgc ctcgctcgat ggcctc
//
LOCUS       DASRJQ010000217         6985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2607069, whole genome shotgun sequence.
ACCESSION   DASRJQ010000217 DASRJQ010000000
VERSION     DASRJQ010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6985
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2607069"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="VFB89_04865"
     CDS             <1..1081
                     /locus_tag="VFB89_04865"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HZM26663.1"
                     /translation="VTGSHNPPEFNGFKMVLAGDAIHGDEILELWEIIVAERWRTGEG
                     TETVDGSVLRKYQDGIVSRNHLRRAVRAVVDCGNGTGSVIAVPTLQALGAEVMPLFCE
                     SDGTFPNHHPDPTVPENLKDLQAAVRRSGAELGIAFDGDADRIGAVDENGEIIFGDQL
                     LVIFGRDAVRRFGPGTPVIFDVKCSQVLPDALERAGARPVMWKTGHSLIKAKMKETHA
                     PLAGEMSGHMFFGGDYLGFDDALFAAARLFEVVSNNSGGLATLLADLPKTFATPEIRV
                     ECPEEVKFAIADRAVAHFSAKYPVNTIDGVRMTFPKGWGLIRSSNTQPVLVLRFEATD
                     PKSLEAYRGEVMGWLAEQGVKTGTA"
     gene            1261..3738
                     /locus_tag="VFB89_04870"
     CDS             1261..3738
                     /locus_tag="VFB89_04870"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HZM26664.1"
                     /translation="MSRRARQVLALLVIVGVLLFVGRWVAGLLADRWWAEGISPAAVT
                     FITNWRLLSLTLELGGILVATAWFIGHLLVVYRAIGSVQISRHVANLEIREALTAEVL
                     LGTAIGMGLLLGLLAGAGTSSWTPAVALAWRGVTFGVREPVLGHDLGLYVAQLPLWRL
                     LHGFAVLLCSLGLITCVALYSLVGALRWSGGRPAINDHARIHLGILLVCLALALAWGY
                     LLEPYELVAGLSGAPDRPAVEMAALVAPALTGTALMVAVMSAIWAARATHALVAASWL
                     VLVITSIGGHYVLPAFNRDRGAPAVEPELTRQFERIGFGLSDLHDSAGSRLLRDVSPP
                     RVAPLWGDHVVARLAGDISPDSVTAHPGGLAVEGRRLQVWLVDRSSLADKPGILAVAA
                     DRVAPGGDPLYYRLGDTLGYPTPFALQLQLDSSAVQPGAPPYDVAQNGPGILLDAWPR
                     RLILAWALQAGQLLTALPQGARLVWHLSPEERFGALLPFADWGEAVPVVVDNELVWVS
                     DGYVTSETFPLIDRLPWRRDAVNLVQAGFVGVVWARTGRTAIYLRGNAETEGLARTWA
                     AIAGGVVQPIRDLPAGLAAALPYPDELFAVQARALEREPWDAGTLSDRPEAGPGEPGP
                     PTPGWRADTGGTRLVALYERPSGGRVSAALAGATRGGRPVLDLVRLDSVRSLPAPRAL
                     DATWRRFPSFQSLADSVRSTGAPRGDSLVRGPLTVWLDPVAGLGAYRTWYAPRGNALA
                     LVWVSIAQSGRPVGGAGRTLAEAWDNLRGASAPLPPTVPTASLEEARKWLRIADSALR
                     AGDWAGFGRAFDALKQVIGTERDTLAR"
     gene            3802..4740
                     /locus_tag="VFB89_04875"
     CDS             3802..4740
                     /locus_tag="VFB89_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HZM26665.1"
                     /translation="MRTAVLALGGNALARAGERATITNQFRHARESVAPIVELAKDGW
                     GIAIVHGNGPQVGDELVRNEVARKLVEPLPLGVLVANTAGWIGYMLQQSLQNALRFGQ
                     VRRDVVTLLTQTLVDPYDPALSRPTKPIGHALSDELAAQLRETGIAIGLDGAGHWRRL
                     AASPVPTGIVELELIRRLLEQDVIVVAGGGGGPPVYDHPLLGYEGLEAVVDKDRVAAI
                     LARQLAAEVLMILTDVDAVYADWGTPAARPLLRITLEQVDELIVAGGVNEGGMKPKVQ
                     AAADFVRNGGGRAIIARLTDGPAALRGEAGTTIVRA"
     gene            4741..5880
                     /gene="sucC"
                     /locus_tag="VFB89_04880"
     CDS             4741..5880
                     /gene="sucC"
                     /locus_tag="VFB89_04880"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HZM26666.1"
                     /translation="MNIHEYQARALLKAQGIPMLDGDVASTPTEAEAIARRLGRTVVI
                     KAQVHAGGRGKAGGVRLAKNPAEALDHAAEILGMQIKGLTVQKVLVVPAAEIASESYL
                     GLIMDRESQRPVFMVSSAGGVDIEEVAAKTPEKIVRLGVDPTYGLFPHQALALAFALY
                     QDFAQVKAAAKIMEQLYTAFLANGASLAEINPLVTTPDGRVLALDAKIVIDDNELFWR
                     PDLGLLRDDTAEEPSEAAARRANLSFIKLDGNVGCVVNGAGLAMATMDLVKFYGGEPA
                     NFLDIGGSSSPEKVINALKIITQDAHVKAILFNIFGGITRTDDVANGIVAATRQFKVN
                     VPIVIRLTGTNEAEAIKILNGVGMRALSDMDQAVEQVVKLAREAA"
     gene            5877..6749
                     /gene="sucD"
                     /locus_tag="VFB89_04885"
     CDS             5877..6749
                     /gene="sucD"
                     /locus_tag="VFB89_04885"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HZM26667.1"
                     /translation="MSIFANDNTRLLIQGITGREGSFHARQMLEYGTNVVGGVTPGKG
                     GQKFEQKVPVFNTVAQAVEATGANTSVIYVPPMAAAAAIVEAADASIPLVVCITEGIP
                     VMHMSRTMPFVRSRSVRLIGPNCPGLITPGSKAKVGIIPGNICSAGRVGLVSRSGTLT
                     YEVVNHLTRAGIGQSTCVGIGGDPIIGTNFIDCLRAFQEDPATDAIVMIGEIGGTDEQ
                     NAAQFVKTRVTKPVVGFIAGQTAPKGRRMGHAGAIISGSSGTAEEKLAAFEAAGIGIM
                     RRPADVVELLRKRM"
     gene            6746..>6985
                     /locus_tag="VFB89_04890"
     CDS             6746..>6985
                     /locus_tag="VFB89_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26668.1"
                     /translation="MRLADLLSPGRVVVPLAAATWADGIRLLVDACVADGRVRDEAKL
                     EAAVRHAWPEDTLSLGPHAVLPHFRTDAVDGVVAAL"
BASE COUNT         1152 a         2227 c         2376 g         1230 t
ORIGIN      
        1 ggtcaccggc tcgcacaatc cgccggaatt caacggattc aagatggtgc tcgcgggcga
       61 cgccattcac ggtgacgaga tcctcgagct atgggaaatc atcgtcgcgg agcgctggcg
      121 taccggcgag ggaaccgaga cagtcgacgg atctgtgctg cggaagtacc aggacgggat
      181 cgtctcccgc aatcacctgc gccgagcggt gcgagcggtc gtggactgcg ggaacggcac
      241 tggcagcgtg atcgccgtcc ccacgctcca ggcactcggc gccgaggtga tgccgctgtt
      301 ttgtgagtcg gacggcacgt tcccgaatca ccatcccgat cctaccgtgc ccgagaacct
      361 gaaagacctt caggccgcgg ttcggcgcag cggggcggag ctggggattg cgttcgacgg
      421 cgacgcggac cggatcggcg cggtcgacga gaacggcgag atcattttcg gcgaccagct
      481 cctcgtcatc ttcggtcggg atgccgtgcg acgtttcggg ccagggacgc cggtcatctt
      541 cgacgtgaag tgctcgcagg tgctccccga tgcactggag cgtgccggag ccagaccggt
      601 gatgtggaag accggccact cgctcatcaa ggccaagatg aaggagactc acgctccgct
      661 cgccggcgag atgagcggcc atatgttttt cggcggcgat tacctcgggt tcgacgacgc
      721 tttgttcgcc gcggcccggt tgttcgaagt ggtctcgaac aactctggcg gtctggcaac
      781 tcttctggcc gaccttccca agacctttgc cacccccgag atccgggtcg agtgcccgga
      841 agaggtcaag ttcgcaatcg ccgaccgggc tgtggcccac ttctccgcca agtatcccgt
      901 gaacaccatc gacggcgtgc gcatgacgtt cccgaaaggc tgggggctca tccgatcctc
      961 caacacccag ccggtgctcg tgctgcgctt cgaggcgacc gatcccaagt cgctcgaggc
     1021 gtatcggggc gaagtgatgg ggtggttggc ggagcaggga gtgaagacag gtactgcgta
     1081 aacggcggta aaaacgtgaa cggtgaacga tggaaacagc gtgaacggtg aacggtgaac
     1141 ggtaaacggg gtgctctgac gacgaggctg gcctttggcc tccagggaca ccggacaacg
     1201 cgcatacctc gcgactcccg ttcaccgatc accgttcacc gttcacgctt tatctaaaaa
     1261 atgtctcgcc gcgcgcggca ggtcctcgcc ctcctcgtca tcgtgggcgt cctgctgttt
     1321 gtcggacggt gggtcgccgg actgttggcc gaccgctggt gggcggaagg catctctccc
     1381 gcagcagtca ccttcattac caactggcgc ctgctcagtc tgaccctcga gctgggcggc
     1441 attctggttg cgaccgcgtg gttcatcggc cacctgctcg tcgtttaccg tgcgatcggg
     1501 tcggtgcaga tctcccgtca cgtcgccaac ctggaaatcc gtgaggcgct caccgccgag
     1561 gttctgctcg gcaccgcgat cgggatggga ttgctgctgg gcctgctcgc cggtgcgggg
     1621 acctcgagct ggacgcccgc ggtcgcgctc gcgtggcgcg gcgtgacatt cggtgtgcga
     1681 gagccggtgc tggggcacga cctcgggctc tacgtcgccc agcttccgct ctggcggctg
     1741 ctccacggat tcgccgtgct cctctgcagc ctgggcttga tcacctgcgt ggcgttgtac
     1801 agcctggtcg gcgcgctgcg ctggtcgggt ggacgtcccg cgatcaacga tcacgcgcgc
     1861 atccatctcg gcatcttgct ggtgtgcctc gcactcgcac tcgcttgggg ctatctgctc
     1921 gagccgtacg aattggttgc tgggctgagc ggcgcgcccg atcggccggc cgtcgaaatg
     1981 gctgccttgg tggcgcccgc tctcaccggc accgcgctca tggtcgccgt gatgtccgcg
     2041 atctgggccg cccgagcgac ccacgcgtta gtcgccgcca gctggctcgt cctggtaatc
     2101 acctcgatcg gggggcacta tgtgctgccg gcattcaacc gggatcgtgg cgctccggca
     2161 gtcgagcccg agctgacccg tcagttcgag cgcattgggt tcggcctcag cgatctgcat
     2221 gactccgctg gcagtcgcct cctgcgcgac gtgtcgccgc cacgagttgc tccgctctgg
     2281 ggcgaccacg tagtcgcccg actggcgggc gatatcagtc ctgactcggt cactgctcat
     2341 cccggcgggc tcgccgtgga aggaagacgt ctgcaagtct ggctggtgga tcgaagctcc
     2401 ctcgccgaca agccaggcat tctcgccgtc gcggcggacc gggtagcacc cgggggcgat
     2461 ccgctctact accgcctcgg cgatacgctc ggctatccca cacccttcgc gcttcagctg
     2521 cagctggact cgagcgcggt gcagccgggc gcgccaccct acgatgtcgc tcagaatggg
     2581 cccgggattc tcctcgatgc ctggccccgc cgtctgatcc tcgcgtgggc gttgcaggct
     2641 ggccagctcc tcaccgcgtt gccgcaaggc gctcgtctgg tctggcacct ctcgccggaa
     2701 gagcgtttcg gggcgttgtt gccgttcgcc gactggggag aggcggtgcc agtggtcgtc
     2761 gacaacgagc tggtctgggt ctcggatgga tacgtgacct cggagacctt cccgctgatc
     2821 gaccggcttc cctggcggag agatgcggtc aatctggtgc aagcagggtt cgtcggtgtg
     2881 gtctgggccc gaaccggacg caccgcgatc tacctccgcg gcaacgccga aacagaggga
     2941 ctggcccgga cctgggccgc gatcgccggc ggcgtcgtgc aacccatacg cgatctcccg
     3001 gcaggcctgg cagccgccct gccgtatccc gacgagctgt ttgcagtgca ggcccgcgcg
     3061 ctggagcgcg agccatggga tgcagggacg ctcagcgatc gccccgaagc cggacccggc
     3121 gaacccggtc cgcccacacc gggctggcga gcagacaccg ggggcacccg gctggttgcc
     3181 ctctacgaac gtccctcggg tggccgggtg agtgctgcgc tggccggggc gacccgaggc
     3241 ggtcgtcccg tgctcgacct ggtccggctc gactctgtca ggagcttgcc ggcgccgcgg
     3301 gctctcgacg cgacctggcg acgattccct tcgttccaga gcctcgccga ttcggtgcga
     3361 tccaccgggg cgccgcgcgg cgacagcctc gttcgcggtc cgctcactgt ctggctcgat
     3421 cctgtcgccg ggctgggcgc gtatcgcacc tggtacgcgc cccggggaaa tgcgctcgcg
     3481 ctggtgtggg tgagcatcgc gcagagtggt cgtccggttg gtggggcggg gcgaacactg
     3541 gcggaggcgt gggacaatct gcgcggcgcg agtgcaccct tgccgccgac agtcccgacc
     3601 gcttcgctgg aagaggccag gaagtggctg agaatcgccg attcggcatt gcgagcggga
     3661 gattgggcgg gctttgggcg tgcgttcgat gcgctcaagc aagtcatcgg aacggagcgg
     3721 gacacgctgg cgcgctgagc ttgctcgacc ctctaccccg ggctaggttc ctcgcgcctt
     3781 ggcccaggga tctggaggga catgcggacc gcggtgttgg cgctcggagg aaacgcgctc
     3841 gctcgtgccg gcgagcgcgc gacgattacc aatcaatttc gccacgcgcg ggaaagcgtc
     3901 gccccgatcg tcgagctcgc gaaggatggc tggggcattg cgatcgtgca cgggaacgga
     3961 ccccaggtag gcgacgagct cgtccgcaac gaggtcgctc gcaaactggt cgagccgctt
     4021 ccacttggcg tgctggtggc gaacacagcc ggctggatcg ggtacatgct ccagcaatcg
     4081 ctccagaacg cccttcgctt cggccaggtt cgccgtgacg tggtcaccct gctcacgcag
     4141 acgctggtcg acccatacga tcccgctctc tcccgtccaa ccaaaccgat cggtcatgcg
     4201 ctctccgacg agctggcggc gcagctgcgg gagaccggca tcgcgatcgg cctggacggc
     4261 gccgggcact ggcgccggct cgcggccagc ccggtgccga ccggcatagt ggaactcgag
     4321 ctgatccgac gactcctcga gcaggacgtc atcgtggtgg cgggcggcgg cggcggtccg
     4381 ccggtgtacg accatcctct gctgggctac gaggggctgg aagcggtggt ggacaaggat
     4441 cgcgtcgccg cgatcctcgc acgccagctc gccgccgagg tcctgatgat tctcaccgat
     4501 gtcgacgcag tctatgcgga ttggggaacg cccgctgcgc ggccactgct ccggatcacg
     4561 ctggagcaag tggatgagct gatcgtggcc ggtggtgtca atgaaggcgg gatgaaaccc
     4621 aaggtacagg cggctgccga tttcgtgcgg aacggcggcg gccgcgccat catcgcgcgg
     4681 ctcacggacg gcccggcggc cctccgggga gaagcgggaa cgaccattgt gagggcgtag
     4741 atgaacatcc acgagtacca ggctcgggcg ctgctcaagg cccaaggcat ccccatgctc
     4801 gatggcgatg tcgcgtcgac acctaccgaa gctgaggcca tcgcacggcg tctgggccgg
     4861 accgtcgtca tcaaggcgca ggtgcatgcc ggtggtcgag gaaaggcggg tggcgtccgg
     4921 ctggccaaga atcccgccga ggcactcgat cacgccgccg agattctcgg catgcagatc
     4981 aagggactca cggtgcagaa ggtactcgtg gtaccggcgg cggagattgc ctcggagagt
     5041 tatctcggcc tcatcatgga tcgggaatcg cagcgccccg tgttcatggt cagctcggcc
     5101 ggaggagtgg atatcgagga agtggccgcc aagactccgg agaagatcgt ccgtctgggc
     5161 gtcgatccca catacggcct ctttccccat caggcgctgg cgctggcctt tgcgctttac
     5221 caggacttcg cccaggtgaa agccgcggcc aagatcatgg agcagctcta taccgcgttc
     5281 ctcgccaacg gtgcatcgct cgccgaaatc aatccgctgg tgaccacgcc ggatgggaga
     5341 gtgctggcgc tcgacgcgaa aatcgtgatc gacgacaacg agctcttctg gcgcccggat
     5401 ctgggtctgc tccgcgacga tactgcggaa gagcccagcg aggcagcggc tcggcgggcc
     5461 aacctctcgt tcatcaagct cgacggaaat gtcggctgcg tggtcaacgg agctggtctc
     5521 gcgatggcga cgatggacct agtgaaattt tacggcggtg agccggccaa cttcctcgat
     5581 atcggcggca gctcgagtcc tgagaaggtc atcaacgctc tcaaaatcat cactcaggat
     5641 gcccacgtaa aagcgatcct cttcaacatc ttcggtggga ttacccgcac cgacgacgtc
     5701 gccaacggga tcgtcgctgc gacgcgccag ttcaaggtga acgtccccat cgtcatccga
     5761 ttgaccggca cgaacgaagc cgaggcgatc aagattctga atggggtggg aatgagagcg
     5821 ttgagcgata tggatcaggc ggtcgagcag gtggtcaagc tggcgcggga ggcggcatga
     5881 gtatcttcgc caacgacaac actcgcctgc tgatccaggg tatcaccggc cgcgaaggct
     5941 cctttcatgc gcgccagatg ctggaatacg gaaccaacgt cgtcggcggg gtcactcccg
     6001 gtaaaggcgg ccagaagttc gagcagaaag taccggtgtt caacaccgtg gcgcaggcgg
     6061 tcgaggcgac gggcgcgaat acgtccgtga tctacgtacc gccgatggcc gccgccgctg
     6121 cgatcgtcga agcagccgac gcgtcgatcc cgctggtcgt gtgtatcacg gaaggcattc
     6181 ccgtcatgca catgagcagg accatgcctt tcgtgcgaag ccgaagcgtc cggctgatcg
     6241 gtccgaattg tcccggtctc atcacgccgg gctccaaagc caaagtgggc attattcccg
     6301 gcaacatctg cagcgcaggc agagtgggac tggtttcccg cagcgggacg ctcacgtacg
     6361 aggtggtaaa tcacctgacg cgggccggca tcgggcagag cacatgcgtc ggcatcggcg
     6421 gcgatcccat catcggaacg aacttcatcg actgcctgcg cgcgttccag gaggacccgg
     6481 ccacggacgc gatcgtcatg atcggtgaga tcggcggcac tgatgagcag aatgcagctc
     6541 agttcgtgaa gacccgcgtc accaagccgg tggtcggctt cattgccggg cagaccgcgc
     6601 ccaagggacg gcgaatgggc catgccggcg ccatcatttc cggttcgtcg ggcaccgccg
     6661 aggagaagct ggctgcgttc gaggccgcgg gcatcggcat catgcggagg ccggctgacg
     6721 tggtcgagct gttgcggaag cgaatgtgag gctggcagat ctgctgagcc ccgggcgggt
     6781 cgtcgttccg ctcgctgcag cgacctgggc tgacgggatt cggctcctgg tcgatgcctg
     6841 tgtggccgac ggtcgggtga gggacgaagc caaactcgag gcggcggtgc gccatgcctg
     6901 gccggaggat acgctgagcc ttggcccgca cgcggtactt ccgcacttcc gcaccgatgc
     6961 cgtcgatggt gtggtggccg cgctc
//
LOCUS       DASRJQ010000218         2780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2976489, whole genome shotgun sequence.
ACCESSION   DASRJQ010000218 DASRJQ010000000
VERSION     DASRJQ010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2976489"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VFB89_04895"
     CDS             complement(<1..386)
                     /locus_tag="VFB89_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26669.1"
                     /translation="MATRTTMSPTAEVSPPIARLRGYGAYQQPRWNIEAGPIGPVDIT
                     GTTARSISGAHGKCRKAADHIIREHNDREGGESSESGLGLTPAIIEQVARASLRRAHR
                     TVLRMTRSAFAWTIEVRMANATTGTEA"
     gene            411..1460
                     /locus_tag="VFB89_04900"
     CDS             411..1460
                     /locus_tag="VFB89_04900"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HZM26670.1"
                     /translation="MAITRPGRAVGENLRRAARWIVGIALVAALVWQVDLGEAARHLL
                     NAHRGGLLLALLVLGLSRFVKAARSYPLMQVQSNQLPFWLCLRAYLASGSAAYLLPSG
                     FGADVLRVVALGRGRGLVPEVTASIAMERMLSGVALVTANLLAALYALQGRQPGRFAL
                     GSLGLAVAAAIAMVLPLNERFMEWIRRSWIARKLSRYADSRWMHTITRFWNAYYAYRH
                     RPGVLLAVMLLSLTETVLVVISIAFLAAATGTGITPAMLLVVVPLTLVLHRLPITYWG
                     IGVADGGFAFLLKALYFIPPTQALAVAAAYRVIELLANIPGALLWRDLASASRAPGPA
                     AESGRKARIPVGVGR"
     gene            1908..>2780
                     /locus_tag="VFB89_04905"
     CDS             1908..>2780
                     /locus_tag="VFB89_04905"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZM26671.1"
                     /translation="MPSLEGVVSAALAGRYQIERELGHGGMATVYLARDAKHDRQVAL
                     KVLRPELTATLGSDRFLREIQVSARLTHPNILAVYDSGNSNGVLYYVMPYIEGESLRA
                     RLSRETRLGIAESIGLGREMADALDYAHSRGVVHRDVKPENVLLAAGHAVIGDFGIAR
                     AMHVAGVTHVTAEGVIIGTPAYMSPEQAGGERDIDGRSDIYSLGCLLFETLVGSTPFT
                     GNSAQTLLVRRLTEDAPLLRTIDSSIPVALEAVVAKALATDPADRFATAGELSGALDR
                     VERLGPGAAVVPGPS"
BASE COUNT          424 a          902 c          910 g          544 t
ORIGIN      
        1 gcttccgttc cggtggtcgc gttggccatt ctgacctcga tggtccacgc gaaggcgctc
       61 ctggtcatcc gaaggacagt tcgatgcgcc cgccgcagcg acgcacgcgc cacctgctcg
      121 atgatcgccg gcgtgagacc gagcccactt tctgagcttt cccccccttc gcggtcattg
      181 tgctctcgaa ttatgtgatc tgcggccttt cggcacttcc cgtgagcacc gctaattgac
      241 cgcgcagtag ttccggtgat gtctaccgga ccgatcggtc cggcttcgat gttccaccgg
      301 ggctgttgat acgccccgta tcctcgcaat cgcgcgatcg ggggagatac ctctgccgtg
      361 ggggacatcg tggtacgggt ggccaccgga acaagtttgt agcgtcgatg atggccatca
      421 cacgcccggg ccgcgcggtt ggggaaaacc tccggcgcgc agcgcgctgg atcgtcggga
      481 tagcgttggt cgccgcgctg gtgtggcagg tagatctagg cgaagccgcc cggcatctgc
      541 tgaatgctca ccggggcgga ctgctgcttg cgctgctggt cttggggctc agccgcttcg
      601 tcaaggcggc ccgctcgtac ccgctgatgc aagtgcagag caaccagttg ccgttctggc
      661 tctgcctgcg tgcgtacctg gcatcaggat cggcggccta cctgctcccg tctggtttcg
      721 gggcggatgt gctccgcgta gtggcgctgg gccgcggccg cggcctcgtt ccggaagtca
      781 ctgcatccat cgccatggag cgaatgctga gcggtgtcgc gctggtgacg gcgaacctcc
      841 tagccgccct ctacgcgctt cagggccgcc agccgggacg ctttgccctg gggtcgttgg
      901 ggcttgcggt cgcagcggcc atcgcaatgg tgcttcccct caacgagcgg ttcatggaat
      961 ggattcggcg cagctggatc gcccgcaagc tgtcacgata cgctgacagc cgttggatgc
     1021 acaccatcac acggttctgg aacgcctatt acgcctaccg gcaccggccc ggcgtcctgc
     1081 tggccgtcat gctgctgtcc ctgaccgaga cggtattggt cgtgatcagc atagcattcc
     1141 tggcggctgc caccggaact gggatcaccc ctgcgatgct gctcgtcgtc gtcccgctta
     1201 cactcgtgct gcaccggctg ccgatcacct actggggcat cggtgtcgcc gatggcgggt
     1261 tcgcattcct cctcaaggcc ctctacttca tcccgccgac tcaggcgctc gccgtcgccg
     1321 ccgcgtatcg cgtcatcgag ctgctggcga acatccccgg agctctcctg tggcgcgatc
     1381 tcgccagcgc gagccgggct ccgggtcccg cagcggagtc gggtcgcaag gcaaggattc
     1441 cggtaggcgt aggtagatag gcgataggtc agcggcgggc acgaggtcga ccgtccagag
     1501 accgcgcgga cgcctagtgt gaagtaaagc cgcttgccat cggttacgaa ttcgggctgg
     1561 tgagatggcc gtgtcgggtc atcgaaagcc gtcctacaaa gatgcactgc ttcatggtgg
     1621 cgctccccac acatacatga tggtggtcac gcgggctcgt gcatcgaggt tctggaaccg
     1681 atgctggcga tcccgtctcc agtgatcgcc gcgccgcggg cggacctgct tggggtgctt
     1741 ctccgggaac atcccaggtt ctgccaaccg atcgcggagg gcggcggccg agggactagc
     1801 ggtagattgg acgttcatgg atgaggcggc cagttgttca ataccatcgc gttcgtcgag
     1861 agcgggacgc tctgcgaccg gctccagggg ggctgcccct gacagccatg cctagcctcg
     1921 agggtgtcgt gagcgctgcg ctcgccggcc ggtaccagat cgaacgcgag ctcggccacg
     1981 gcggcatggc gactgtctac ctggctcgcg acgcgaagca cgaccgccag gtcgccttga
     2041 aggtgctacg gcccgaactg accgccacgc tgggctccga tcgcttcctg cgcgagatcc
     2101 aggtctccgc gcgccttact catccgaaca ttcttgcggt gtacgactcc ggcaactcta
     2161 atggtgttct ctactatgtc atgccgtata tcgaaggcga gtcgttgcgt gcgcgcttga
     2221 gccgcgagac ccgtctcggc atcgcagagt caattgggct gggccgcgag atggccgatg
     2281 cgctcgacta cgctcatagt cggggagtgg ttcaccgcga tgtcaagccg gaaaacgtcc
     2341 tgctggcagc cggtcatgcc gtcatcggcg acttcggaat tgcgcgggcc atgcatgtag
     2401 cgggtgtcac ccacgtcacg gccgagggag tgatcatcgg gacgcccgcg tacatgagcc
     2461 cggagcaggc tggtggcgag cgcgacatcg atggtcggag cgacatctac agtctgggct
     2521 gcctcctgtt cgagaccctg gttggtagta ccccgtttac cgggaacagc gcccagacgc
     2581 tgctggttcg tcggcttacc gaagacgccc ccctgctgcg caccatcgat tccagcatcc
     2641 cggtcgctct cgaagcggtc gttgccaaag ccctcgccac cgacccggcg gaccgctttg
     2701 cgaccgcagg cgagctatcg ggggcccttg atcgggtcga gcggttggga cccggcgcgg
     2761 ccgtcgtccc tgggccatcc
//
LOCUS       DASRJQ010000219         3473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2565962, whole genome shotgun sequence.
ACCESSION   DASRJQ010000219 DASRJQ010000000
VERSION     DASRJQ010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2565962"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VFB89_04910"
     CDS             <1..273
                     /locus_tag="VFB89_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26672.1"
                     /translation="DRVEFLDGDAADLAPLLGPCEIVLSNILRSVNLRLLPVITAALA
                     PAGTAIFAGMELPEAEIFRPVLERAGLAVIDEAVDAGWWAVAARQG"
     gene            270..983
                     /locus_tag="VFB89_04915"
     CDS             270..983
                     /locus_tag="VFB89_04915"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HZM26673.1"
                     /translation="MITVLLPPGRVSVGDVVPLDPAETHHLRVRRAGEAISVRLRDGR
                     GLVGAGALRDERGSVAVVVEQAEMATRPAPLRLAVAAGDRDRFAWLVEKATELGVTDI
                     FLITTERTAAVATRMREGQLARVRRRAFEAVKQCGAAWAPEVHPAQPMAGLLAQRLEG
                     ARWVADVDGRAVPPAFGAGPCTILVGPEGGLSRAEQDSVAAAGFLAVRLAANVLRFET
                     AALAAAACAAAARAGGGDG"
     gene            976..1323
                     /locus_tag="VFB89_04920"
     CDS             976..1323
                     /locus_tag="VFB89_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HZM26674.1"
                     /translation="MADCIFCKIASGEISGGVVKQTPEFVAFRDMKPMAPTHVLVVPL
                     AHISAARDAKGEAGQRMLGGLLAFATQVASELGLDAGGYRIVTNTGRDGGQSVDHLHL
                     HVLGGRAMGWPPG"
     gene            1486..1683
                     /gene="rpsU"
                     /locus_tag="VFB89_04925"
     CDS             1486..1683
                     /gene="rpsU"
                     /locus_tag="VFB89_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005805980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HZM26675.1"
                     /translation="MSEVIIHDDESFERALKRFKKKCEKAGILSDLRKHRHYEKPSEK
                     KKRKMNAAIRKTRRGPRISRV"
     gene            1676..2116
                     /locus_tag="VFB89_04930"
     CDS             1676..2116
                     /locus_tag="VFB89_04930"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HZM26676.1"
                     /translation="MSNLTDALRAALNAARKAQQKDRTLVLGTILANVKNRELELGRD
                     PTDDEVVEVLRKGIKIRREAHDQYSAAQRSDLAEVELSQVKILEEFLPPAAQPEEIRA
                     AARAAIEAGATDIGKVMGVVMPRFKGRADGKLINQIVREELQAG"
     gene            2113..>3473
                     /locus_tag="VFB89_04935"
     CDS             2113..>3473
                     /locus_tag="VFB89_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26677.1"
                     /translation="MMKGQVEVNAFSGGVPEDTTTGTPPLLADAGTADALETIELRAV
                     LARVAAFAAGPLGADRVRARRPTHDIGWIRIELARVQEVAALFRKGDGLLAEAVPDVA
                     PALARLRIEGSVLEGIELAGIHRALLASQAVHGDLSRVASQAPRAAELLRPLPDRRLE
                     RRLEQSVDADGSLLDSASPRLAAARREVLDARNRLVRRLEGLLRGLESNAVPPNATVT
                     VWDGRYVIPVRRDSRARPSGIVHGESGSAGTLFVEPTEAIDLGNALREAQVEEERETL
                     RVLRELTALLRPALSVIRDAVEMTIAVDDLVARARYALAVAAEVPVVEEAPAALAIRS
                     GRHPLLLSGSAPVVPFDLDLDVAERTLLVSGPNTGGKTVLLKAVALLAALAQSGIVPP
                     VGAESRLPVFGQFFADIGDRQSIAASLSTFSAHVAALRRILDETDATSLVLLDEIGSG
                     TDPT"
BASE COUNT          624 a         1032 c         1224 g          593 t
ORIGIN      
        1 gacagggtgg agttcctcga cggagatgcg gccgaccttg caccgctgct cggaccgtgt
       61 gagatcgtgc tctccaacat tctgcgaagt gtgaacctcc gactgcttcc ggttattacc
      121 gccgctctcg cacctgcggg aaccgccatc tttgcgggta tggaattgcc cgaggcagaa
      181 atcttccggc ctgtgctcga aagagccggg cttgcagtca tcgacgaagc ggtcgatgcg
      241 ggatggtggg cggtggcagc gcggcaggga tgatcacggt tctccttcct ccgggacggg
      301 tttccgtcgg cgacgtagtc cctctcgatc cagctgagac tcatcatctc cgggtacgcc
      361 gggccggcga agcgatcagc gttcggctcc gggatgggcg gggcttggtg ggagcaggcg
      421 cgctgcggga cgagagagga tcggtagctg tcgtcgtcga gcaggcagag atggcgaccc
      481 ggccagcccc cctccgtctc gcggtcgcgg ccggggaccg cgatcgtttc gcctggctgg
      541 tcgaaaaggc caccgagctc ggcgtgaccg acatcttcct gatcaccacc gaacgcacgg
      601 ccgcggtagc aacccggatg agagaaggtc agttggcgcg agtcaggcgt cgggccttcg
      661 aggcagtgaa acagtgcgga gcggcgtggg cgccggaggt ccatccggcc caaccgatgg
      721 ccggcctact cgcgcagcga ctcgagggcg cacgctgggt tgccgatgtc gatggacgcg
      781 ctgtcccccc ggcctttggg gccggtcctt gcacgattct ggtcggcccg gaaggcggcc
      841 tgagtcgagc ggagcaggac agtgtcgctg cagccggctt tcttgcggtt cggctggctg
      901 caaacgtgct gcgattcgaa actgcggcgc tggcggcggc ggcatgtgcc gctgccgcac
      961 gtgcgggagg aggcgatggc tgactgcatc ttctgcaaga tcgcgagcgg tgaaatcagt
     1021 ggcggtgtgg tgaagcaaac gccggagttc gttgcattcc gggatatgaa gccaatggcg
     1081 ccgacccatg tgttggtggt tcctctggct cacataagcg cagcgcgcga cgccaaaggc
     1141 gaagcggggc agcgtatgct gggcggccta ctcgcgtttg ctacgcaggt tgcaagcgag
     1201 ctggggctcg atgcgggcgg ctatcggatc gtgaccaata ccgggagaga tggcggccag
     1261 agcgtggatc atctgcacct gcacgtgctg ggcggccgtg cgatggggtg gcccccggga
     1321 tagagagaac gggggaaaga gagaaaggga gaaaggggaa tgcggtgtgt gccaactgag
     1381 ttctcccgtt ccccattccc cctttctcct gcggtctaac ctctctagat tgttgggctt
     1441 aacgaacccg cccagccagg gcgcgagagg ggtgacttcg ccagcatgtc cgaagtgatc
     1501 attcacgacg acgaaagctt cgagcgcgca ctcaaacggt tcaagaagaa gtgcgagaag
     1561 gccggaattc tgtccgatct gagaaaacat cggcactacg aaaagccgag tgaaaagaaa
     1621 aagcggaaga tgaacgcggc catccgaaag acccgccgcg ggccacggat ctcgcgtgtc
     1681 taacctcacc gacgccttgc gggccgcgtt gaacgcggcc cgtaaggcgc aacagaaaga
     1741 ccggacactc gtactcggaa ccatcctcgc caacgttaag aaccgcgagc tcgagctggg
     1801 tcgcgatccc acggacgacg aggtcgtaga ggtactccgg aagggaatca agatccgccg
     1861 tgaagcacac gatcaataca gcgcggcgca gcgctccgat ctcgcagagg tggagctgag
     1921 tcaggtcaag attctggagg aattcctgcc gcccgcagcg caaccggagg agattcgagc
     1981 ggcggcacgt gcggcgatcg aagccggcgc gaccgacata ggcaaggtga tgggagtggt
     2041 gatgccgcgc ttcaagggcc gagccgacgg aaagctgatc aatcagatcg ttcgcgagga
     2101 gctgcaggcg ggatgatgaa ggggcaggtg gaagtcaacg ctttttcagg cggggtgccg
     2161 gaggatacaa ccaccggcac cccgccgctt ttggctgatg ctggaactgc cgatgccctc
     2221 gaaaccatcg agctgcgcgc cgtgctcgcc cgagttgccg catttgccgc cggcccactc
     2281 ggcgcggatc gggtgcgggc acgccgtccc acccatgaca ttggatggat tcggatcgag
     2341 ctggcccgcg tgcaggaggt cgcagcgctt tttcgaaagg gagatggcct cctggctgaa
     2401 gcggtgcccg acgtggctcc cgctctcgcc cgactgcgca tcgaggggag cgtcctggaa
     2461 ggaatcgagc tggctggaat tcaccgagcg ctgctcgcca gccaggccgt ccacggcgat
     2521 ctgagtcgag ttgcgtcgca ggcgccccga gccgccgagc tgttgcggcc gctgcccgac
     2581 cgacgcctcg aacgccggct cgagcagtcg gtggatgcgg atggatcgct gctcgacagc
     2641 gcgagccctc gactcgcggc ggcgcggcgg gaagtgctgg acgcgcggaa ccggctggtg
     2701 cgtcgcctgg aggggctgct tcgcggcctc gaaagcaatg cagtaccgcc caatgccacg
     2761 gttacggtgt gggatggacg ctacgtcatc ccggttcgac gcgactcgcg agcccgcccc
     2821 tctggtatcg tgcacggaga atccgggagt gctggaacgc tttttgtcga accgaccgag
     2881 gcaatcgatc tgggcaacgc gctgcgcgag gctcaggttg aggaagagcg ggaaacgctc
     2941 agggttctgc gcgagctgac ggccttgctg cgtccggcgc tttccgtcat ccgggatgcg
     3001 gtcgagatga cgatcgcggt cgacgacctc gtcgcccgcg cgcgctacgc gctggccgta
     3061 gcggccgaag tgcccgtggt cgaggaagcc cctgcggcgc ttgcaatacg aagcggtcgg
     3121 catccgctgc tgctgtccgg ctcggcaccc gtggtgccgt ttgatctcga tctggatgtt
     3181 gcggaacgca cgctgctggt aagcggtccg aataccggcg gcaagacggt gctgctcaag
     3241 gcggtcgcat tgttagccgc gttggcacag agcgggatcg tgccgccggt tggtgcggaa
     3301 agccggctac cggtgttcgg gcagttcttt gcggacatcg gagatcggca atccatcgcg
     3361 gcgagcttgt cgaccttcag cgctcatgtc gcggcgctgc gccgcatttt ggacgagact
     3421 gatgcgacgt cgctggtcct gctcgacgaa atcgggagcg gcacggaccc gac
//
LOCUS       DASRJQ010000220         4501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_351471, whole genome shotgun sequence.
ACCESSION   DASRJQ010000220 DASRJQ010000000
VERSION     DASRJQ010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351471"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="VFB89_04940"
     CDS             <1..855
                     /locus_tag="VFB89_04940"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HZM26678.1"
                     /translation="PTFILAGGLPWHTMVGFYYSTNSTLVPLYPNEWEFFGRVRPISQ
                     DDGGPLDVAGQVGYNLAADGLDGEISLARRFGPVRPIAVLRILKNPNADGTDVAVGGG
                     AVVRLFRYLALAGDVASVTERDPGEKVSWSAGLQIGIPSTPHSLSLHASNASTVTLEG
                     ASRGGDHTRYGFEFTIPFTLARYFGRKTKPAAAPAATPAPVGNVVHTTMLNLAFTTAK
                     LEVPVGTTVEWKNEDQVPHTVTANDKSFDSGTMDADAVWQHKFDRPGTYTYFCTLHPF
                     MKGTVIVR"
     gene            complement(1137..1207)
                     /locus_tag="VFB89_04945"
     tRNA            complement(1137..1207)
                     /locus_tag="VFB89_04945"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1173..1175),aa:Gln,seq:ttg)
     gene            complement(1251..2132)
                     /gene="ispE"
                     /locus_tag="VFB89_04950"
     CDS             complement(1251..2132)
                     /gene="ispE"
                     /locus_tag="VFB89_04950"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HZM26679.1"
                     /translation="MSQVAVACHAKLNLFLRVLAREEDGFHSLETLFCLLELADHLVA
                     ARREEHGVSIEVSGADVGPPAENLAVRGAQLVLEATGDRFGVHLELTKRIPARAGLGG
                     GSSDGAAALLAVNRLAGDAVPRHELLQFAARLGSDVPFLLSGAALALGWGRGERLMRL
                     PALPAAPVLLLTPAVSVSTAEAYLWVDDARRSIGRRGAVALEGGALASWGDIARMAGN
                     DFESPVFARHPPVREAFEALVATRPTICRMTGSGSTLFAIYRSERDRDDAAMMLGRKH
                     GTVMSTRTVATSAKGPE"
     gene            complement(2129..3166)
                     /locus_tag="VFB89_04955"
     CDS             complement(2129..3166)
                     /locus_tag="VFB89_04955"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HZM26680.1"
                     /translation="MPTPTNRSRIWTSLLGVVISAALLFFIIRQVNLAEVRRNLAAAH
                     PIPLLVAVLVATLTFPIRTIRWRILLRSAQGEPLPFAPMWHATAIGFMANNVLPFRAG
                     ELIRPFAATRLAGARFTAALSSIAVERIFDGLTITALLTFALLSPDLPGHVAVGGVSV
                     HHIALVAGSLSAAALLVALAVVAWPLTAERAVRAMVPGVHLADRLVGLIEGIRQGLAA
                     LNSPTRIGGVVFWSFVLWLVNALSFYAGFAAFDINVSFTGALLMQGLLSFGVSVPSTP
                     GYVGPFEAAIVAGLALYGVGEERAFSYAIAYHVTTFIPITLLGLWSVARSPVKLSDLR
                     GPAASISSNSS"
     gene            complement(3150..>4501)
                     /locus_tag="VFB89_04960"
     CDS             complement(3150..>4501)
                     /locus_tag="VFB89_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26681.1"
                     /translation="DWQESDAVRAARLTTLLESLAGRTPLDPQAAGPEPGGSSQLQTG
                     VRAFVAREGALIASALDRAARAIRATPDDCEPLYMVLRRMQSLQGLAELPELPPLPDI
                     LDGIELAVGDLSRLHAPPPGVDEVLAAGARALSRVARDIAETGVPDAEAEEPRSFTAL
                     LLEAFAVERDVVPIESLYRPGDAEPVQRPRSVPQFAAPTPPGSLELVSLGEHLVQTAG
                     RLTQARSVTELDLRLYRLLGTLRAASAPDGDPVAQAIGALARATRETVASGSARGARD
                     QFVAALQQTGELLRGAPGLERAHLAAEINRAAERLNDLRGPVVAIESLAFEGQPALVE
                     SRPAPRAASPSGAAPHAAPTPGRLEAAFSTYSRLRADAPAAPASIDGLLDRKTEELPS
                     PVPIETLCYRGRSAIARAAEIRRELQERLGRDVTMTELRPLIEELLDLVPLALADSD"
BASE COUNT          830 a         1494 c         1483 g          694 t
ORIGIN      
        1 ccgaccttca ttctggcagg cggcctcccg tggcacacca tggtgggctt ctactattcc
       61 accaactcga cgctggtgcc gctttatcca aacgagtggg agttcttcgg ccgggtcagg
      121 ccgatctccc aggatgacgg aggcccactg gacgttgccg gacaggtggg atacaacttg
      181 gcggcggacg ggctcgacgg cgagatctcg ctggcccgcc gcttcggtcc ggtgcggccc
      241 atcgccgtgc tgcggatcct caagaacccc aacgcggatg ggaccgacgt tgcagttggc
      301 ggcggcgcgg tcgtgcgact ttttcgctac ttggctcttg cgggtgacgt ggcgtcggtg
      361 accgagcgcg acccgggaga gaaggtgtcc tggagcgccg gattgcagat cggtattccc
      421 tctacccctc acagtctctc gctccatgcc tccaacgcca gcaccgtgac gctcgaggga
      481 gcctcccgcg gcggcgacca cacccgctac gggttcgaat tcaccatccc gttcacccta
      541 gcgcggtatt tcgggcgaaa gaccaagccc gctgcggcac cagcggccac gccggcgccg
      601 gttggcaatg tcgtccacac gacgatgctt aatctggcat tcaccaccgc caagctcgaa
      661 gttcccgtcg gaaccaccgt cgagtggaag aacgaggacc aggtccccca caccgtgacg
      721 gccaacgata agagtttcga ttctgggacc atggatgctg acgcggtttg gcagcacaag
      781 tttgaccgcc cgggcaccta cacctacttc tgcaccctgc atcccttcat gaagggtact
      841 gtgatcgtcc gatgaggcca ccagggcgtc gtcaacggca gtgtcagcct gcgcggcggg
      901 atgccgcgcg ggccgtttga gcatgcgcac cacgttgttg gatggaattg cattcctctc
      961 acgccttgtc tctcggctct ggctcaggcg ccccgcgact ctgtcgacga gcggggctcg
     1021 cgcagggcct ggggggtcga ttcgtcacca cgatggctgg accgcagcac cgcgccgggc
     1081 gatttcgagg gcttactatc ccaccttact atcccatcca agtgcgaaaa atagcattgg
     1141 ccctggtgga ttcgaaccac cattaacgga tccaaagtcc gctgtcctgc cattggacga
     1201 agggccagaa acccggtgaa gggtaaacgg tgaacagtga aggggtaagg ttactccggt
     1261 ccctttgccg acgtcgcaac tgtgcgggta ctcatgaccg tgccgtgctt tcggccgagc
     1321 atcatcgcgg catcatcgcg atcgcgctcg gagcgataga tcgcaaagag ggtcgacccc
     1381 gagccggtca tgcgacatat ggttgggcgc gtcgccacca gtgcctcgaa tgcctcccga
     1441 accggcgggt gccgcgcgaa gaccggcgac tcgaagtcat tgccggccat gcgggcgatg
     1501 tcgccccaac tggcaagcgc tccgccctcg agcgccaccg caccacggcg accgatactg
     1561 cggcgtgcat catccaccca gagatatgcc tcggcagtgc tcacggaaac cgcgggagtg
     1621 aggagaagca ccggcgctgc cggcagtgcc ggcagacgca tcaaccgctc gccccgtccc
     1681 caacccagcg cgagggccgc gccgctcaat agaaaaggaa cgtcacttcc gagccgcgcc
     1741 gcaaactgaa gcagctcgtg gcgggggacg gcatccccgg ccagccgatt gaccgcgagg
     1801 agcgccgccg caccatcgct cgacccgccg cccaggcccg cgcgagcggg tattcgcttg
     1861 gtcagctcga ggtgaactcc gaatctgtcg ccggtagcct cgagcaccaa ttgcgcgccg
     1921 cggactgcaa ggttttccgc aggcgggccc acgtcggccc cgctcacctc gatggacacg
     1981 ccgtgctcct cccggcgagc tgcgactagg tgatcggcca gttcaagcag gcagaacagg
     2041 gtctcgagac tgtggaagcc gtcttcttcg cgggccagga ctctcaggaa gagattcagc
     2101 ttggcgtggc aggcgacggc aacctggctc atgaggagtt ggaggaaatg ctcgccgccg
     2161 gtccacgcag atcgctgagc ttcacgggac tccgcgcgac cgaccagagt cccagcagcg
     2221 taatcggaat aaacgtggtc acgtgatacg cgatcgcata cgagaaggca cgttcttcac
     2281 ccacgccgta cagtgccaga cccgcgacga tcgccgcttc gaacgggccg acgtagcctg
     2341 gggtcgaggg gaccgacacg ccgaaggaca acaagccctg catcagcaga gcgccggtga
     2401 agcttacgtt gatgtcgaac gccgcaaagc cggcgtaaaa cgacagagca tttaccagcc
     2461 agagcacgaa ggaccagaat acgacacccc cgattcgcgt gggagaattg agtgcggcga
     2521 ggccctggcg aatgccttcg atgagcccaa caagccgatc agccagatga acccccggca
     2581 ccatggcgcg aaccgcgcgc tcggcggtca acggccaggc aaccacggcc agtgccacca
     2641 gcaatgccgc cgcggagagg cttcccgcaa ccagcgcgat gtgatgcacc gatacaccgc
     2701 ccacggcgac gtggccgggt agatcggggc tcagcagcgc aaaggtgagc agcgccgtga
     2761 tggtgagtcc gtcgaagatc cgctcgaccg cgatcgacga cagggccgcc gtaaagcgcg
     2821 cacccgccaa tctggtagcc gcgaacgggc gaatcagctc tccagcccgg aacggcagca
     2881 cgttgttcgc catgaacccg atcgccgtgg cgtgccacat cggagcgaag ggcaggggct
     2941 caccctgggc gctgcgcagg aggattcgcc accggatcgt ccgaatggga aaggtgagag
     3001 tggcgaccag caccgccacc aacagcggga tcgggtgtgc ggcggccaaa ttacgccgca
     3061 cttccgccag gttgacctgg cgaatgatga agaagagcaa cgccgcggat atgaccacgc
     3121 caagcagcga ggtccagatc cgtgagcgat tagtcggagt cggcaagtgc cagcggaacg
     3181 aggtcgagaa gttcttcgat gagtggccgc agctcggtca tcgttacgtc ccggccgagt
     3241 cgttcctgca gctcgcgtcg gatctcggcg gcacgcgcga tcgcgcttcg ccccctgtag
     3301 cacaacgttt cgatcgggac cggggaagga agctcctcgg tcttccggtc gagcaagccg
     3361 tcgatcgatg ccggcgccgc gggtgcgtcc gcgcggagtc ggctgtaggt ggagaaggcg
     3421 gcttccaagc ggcctggggt tggtgccgcg tgcggggccg ctccgctcgg tgaagccgcc
     3481 cgcggggcag ggcggctctc gaccagcgca ggttgtccct cgaacgccag agactcgatg
     3541 gcgaccaccg ggccgcgaag atcgttgagg cgctccgcgg ctcgattgat ctctgctgcg
     3601 agatgggctc gttcgagtcc gggcgcaccc cgcagcaact ctcccgtctg ctgcagcgca
     3661 gccacgaact ggtcccgggc tccgcgggcg ctgccactgg cgacggtctc acgcgtcgct
     3721 cgcgcgagcg ccccgattgc ctgcgcgacc ggatcgccgt ccggcgccga agcggctcgc
     3781 agcgtgccca gcaagcgata cagtctgaga tcgagttcgg ttaccgagcg tgcctgggtc
     3841 aatcggccgg cggtctgaac cagatgctcg cccagactga ccagctcgag cgaacccggc
     3901 ggcgtcggtg cggcgaattg aggcaccgag cggggacgct gcaccggctc cgcatcgccg
     3961 gggcggtaga gcgactcgat ggggaccaca tcgcgctcga ccgcgaatgc ctcgagcaga
     4021 agcgccgtga agctgcgcgg ctcttcggct tcggcgtccg ggactccggt ctcggcgatg
     4081 tcccgagcca cccgcgagag ggcccgggcg cccgcggcca gcacctcatc gacccccggc
     4141 gggggcgcgt gcagtcgcga gagatctccc acagccaact cgataccgtc cagaatatcg
     4201 ggaagcggcg gaagttcggg cagctcggcc agtccctgga gtgactgcat tcgccggagc
     4261 accatgtaaa gcggctcgca atcgtcgggg gtggctcgaa tcgctcgtgc cgcccgatcg
     4321 agtgcgctgg cgatgagggc gccctcgcga gcgacgaacg cgcgcacgcc ggtttgaagc
     4381 tgggacgaac cacctggctc gggaccggct gcttgagggt cgagcggggt ccgaccggcg
     4441 agcgactcga gcaacgtcgt gagccgggcg gcgcgcaccg cgtccgactc ctgccaatcg
     4501 g
//
LOCUS       DASRJQ010000221         2336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2452430, whole genome shotgun sequence.
ACCESSION   DASRJQ010000221 DASRJQ010000000
VERSION     DASRJQ010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2452430"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..354)
                     /locus_tag="VFB89_04965"
     CDS             complement(82..354)
                     /locus_tag="VFB89_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26682.1"
                     /translation="MKIKDLGCDLMPQISFVNLLIVAAVALLAPLLIGYLPRLRLPAV
                     VVEIAAGVVLGPSVLALGADRPTGTDTGRPRAGFLVVSGRVGDRLA"
     gene            556..1572
                     /locus_tag="VFB89_04970"
     CDS             556..1572
                     /locus_tag="VFB89_04970"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HZM26683.1"
                     /translation="MSDNVYIQSNEPDGNRVLAFRRDPDGTLSQRGTFATGGAGSGAP
                     HLQSQGSVVLTRDGRHLLVTNAGSGDLSIFETGEEALTLLQTIPSGGGAPRSVTEHGG
                     LVYVMNTEGPSLAGFRIQEGGLDPVPDTPRALPDGSDPAQVGFIPDGSGIVVTNRGTD
                     SITLYPVDDSGRLGQPTENPSSGPTPYGFAFGTNGTLVVTEAFGAQKGKAAASSYVLG
                     SGSIRPVSRSVGNGRSEICWAVVTSEGRYAFTTNFADGAVSRYAVNADGSLSLEDATA
                     GIAVDGETGLRDEDLSTDGRFIYVLNADSRRIFGWAVGADGRLSQVGAWDGLPASAAG
                     LAAS"
     gene            1576..2016
                     /locus_tag="VFB89_04975"
     CDS             1576..2016
                     /locus_tag="VFB89_04975"
                     /inference="COORDINATES: protein motif:HMM:NF023010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 family protein"
                     /protein_id="HZM26684.1"
                     /translation="MQLKPLYRATFTTPERWAVETSGTHGTEVQDFLIAEGRTEGRLS
                     ARLRGSNYPRRRADGTLLADFRGALDADDGATILFSWHGYARPGAPDRKEIVGSITHV
                     TGNERYAWLNNIVCAMSGEVRPRKGGEDFEVVFDVAELIWEPLD"
     gene            complement(2094..>2336)
                     /locus_tag="VFB89_04980"
     CDS             complement(2094..>2336)
                     /locus_tag="VFB89_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26685.1"
                     /translation="LPLGGCTATNIGTAEIHTVGRMAELMSAAMPGPPPVTTGQYRLG
                     DVRHITADSSKAAQVLGWSSRIRLEDGIADLVHAEA"
BASE COUNT          440 a          742 c          765 g          389 t
ORIGIN      
        1 agtcccgcgc cgaccgctac gccgagcacg agcgtgagcc catatccaag aacggcgaaa
       61 cgtaaggcac gaccgcgcaa cctacgcaag tcgatctcca acccggccag aaacaacaag
      121 aaagccagcc cgaggacggc cagtatctgt accggtaggt cgatctgcac ccagggcgag
      181 gacagagggg ccgagcacga ccccggccgc gatctcgacc acgaccgccg ggagccgcag
      241 gcgtggcagg tagccgatga gcagcggggc cagcagggcg acagcggcca caatgagcag
      301 gttgacgaac gatatctgcg gcatcaggtc gcaccccaaa tccttgatct tcatgctggc
      361 cctaccacgg ttgtcaccgg agacttcttc gcgttacacg taaccggcaa caaaaagtcg
      421 aagttgtgct gtgtaacgct ccgggcattc ggctgactga actgcatgga cgctatggat
      481 gagccgcacc gggagctaaa ccgaagcgaa ggtcactagc gccagaactc ttctacgcac
      541 ggaaggagtg cagctttgtc cgacaatgtc tacatccaaa gcaacgagcc cgacggaaac
      601 cgggtgctcg cttttcgccg cgatccggac gggacgctga gccagcgggg cacgttcgcc
      661 accggcggcg cgggatctgg ggcgccgcac ctgcaatcgc aggggtccgt cgtgcttacc
      721 cgcgatggtc gccacctgct cgtgacgaac gccggcagcg gtgacctaag catttttgag
      781 accggtgaag aggcactcac gctgcttcag accatcccta gcggcggcgg ggcgccgagg
      841 agtgtcaccg agcatggtgg tctggtctat gtcatgaaca ctgagggccc gtcgctggct
      901 ggatttcgca tccaggaggg cggcctcgac ccggttccgg acacgccgcg cgcgcttccc
      961 gacggctccg acccggcgca ggtgggtttt atccccgacg gcagcggaat tgtcgtcacg
     1021 aaccgaggca cggactcgat tacgctttac ccggtcgacg attccggccg cctcggccag
     1081 ccgactgaga acccttcctc gggtcccacg ccttacgggt ttgccttcgg gaccaacggc
     1141 actctcgtag ttaccgaggc gttcggggcg cagaagggca aggctgcggc ttcctcgtac
     1201 gtgctcggca gcggctcgat caggccggtc tcccgctcgg tcggaaacgg gcgcagcgag
     1261 atctgctggg ctgtcgtgac gagcgaggga cgctacgcgt tcacgaccaa cttcgccgat
     1321 ggggcggtat ctcgttacgc ggttaacgct gacggcagtc tcagcctcga ggacgcgacc
     1381 gctgggatag cagtcgacgg cgagaccggc ctacgcgacg aggatctgag caccgacggc
     1441 cgtttcatat acgtactcaa cgccgactcc cggcgcatct tcggctgggc tgtcggcgcc
     1501 gacggcaggc tatcgcaggt gggagcatgg gacggacttc cggcatcggc cgccggtctc
     1561 gcagcgagct gagccgtgca gctcaagccg ctgtaccgag caacatttac gaccccggag
     1621 cggtgggccg tcgagacttc gggcactcac ggcacggaag tgcaggactt tctcatcgcc
     1681 gaaggccgca cagagggacg tctctcagcg cgtctgcggg gatccaacta tccccgcagg
     1741 cgcgcagacg gcacactgct ggccgacttt cgcggtgctc tggacgccga tgacggcgcc
     1801 accatcctgt tttcctggca tggctacgcg cggcccggcg ctccggatcg caaagaaatc
     1861 gttggcagca ttacccatgt gactggcaac gagcgctatg cgtggctgaa caacatcgtc
     1921 tgcgccatgt cgggtgaagt gcggccccga aagggcggag aggatttcga ggtcgtcttc
     1981 gacgtcgccg agctcatctg ggagccactg gactagccga gtcccgccgc cgtcggagga
     2041 aatgcatgtc gcgttggttc ccggcacctc gcggcagttc cacaaggtca cgttcaagcc
     2101 tcggcatgga caaggtcagc gatgccgtct tcgagccgga tgcgtgagct ccagcccagc
     2161 acctgagctg cctttgaaga gtcagcggtg atgtggcgta cgtcgccgag gcgatactgg
     2221 ccagtggtga cgggcggcgg tccgggcatg gccgccgaca tcagctcggc catgcgacca
     2281 acggtgtgga tctcggctgt gccgatgttc gtggcggtgc atcctccgag tggtag
//
LOCUS       DASRJQ010000222         3515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3975470, whole genome shotgun sequence.
ACCESSION   DASRJQ010000222 DASRJQ010000000
VERSION     DASRJQ010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3975470"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(243..1121)
                     /locus_tag="VFB89_04985"
     CDS             complement(243..1121)
                     /locus_tag="VFB89_04985"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZM26686.1"
                     /translation="MTVPGAPLPRQVRSIFHPSDFSESSEVAFAHALKIALVAGADLN
                     ILHVSERGNRPWSEFPRVRQMLERWRVLPPGSPRDAVSSLGIQVKKVLARDSDPVAAA
                     LGYLEQNPTDLVVLATHQREGRARWLTKSVGQPIARQSGLMTLFIPWGLEGFVSRGDG
                     GVSLRNILIPVAQEPRPGAAIEAACRAAWGLQCDEVTFTVMYVGEAGDAPALSFEQHE
                     GWTWQRITREGDVADAIAAVARESAADLIVMATEGRHGFLDALRGSTTERVLRQAPCP
                     LLAVPIGPRPSPPAGH"
     gene            complement(1685..>3515)
                     /locus_tag="VFB89_04990"
     CDS             complement(1685..>3515)
                     /locus_tag="VFB89_04990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HZM26687.1"
                     /translation="SDGVKKADLARVGFVAIAAAAVWFHLWEPFARVSVIGLVATLVG
                     GYPIFREAAENVVERKMTMELSMTIALLAALLIGEFFTALIITLFVLGAEILEGMTVR
                     RGRRAIGDLLDFLPRAAAVRDGGEIRQVRADEIRIGDLVLVNPGSRIPVDGTVAGGHS
                     FVDQAPITGESMPVEKSAGAWAYAGTINQSGALEVRAERLGRDTSFGKIVEAVERAER
                     SRAPIQKLADRLAGYLVYFALGAAALTFLITRDVRSTISVVIVAGACGIAAGTPLAIL
                     GGIGRAARQGAIIKGGRYLETLARVDTVIFDKTGTLTLGAPEVEEVLPAAGISERTLL
                     EAAAAAERRSEHPFAKAILAIAESHSISVVEPQDFAYTPGRGITARVDGDEIVAGTRA
                     LLGERQVAAPEDASDDSRGSSAVLIARGGRYLGAIRVADSVRPEAKAAVGGLKAIGIR
                     TILLTGDARNVAEAVAAELGVDELAAELLPEAKQARVKSLVERGRVTAMVGDGINDAP
                     ALMQANVGVAMGSGTDVARESASVVLLGNNLLKFVETVRLARRTRRTILQNFVGTLAV
                     DGVGIGLAAMGLLNPLLAAFIHVSSELVFILNSARLLPTRVAE"
BASE COUNT          637 a         1198 c         1091 g          589 t
ORIGIN      
        1 aatatatgcg ctcgagcgtc agagaatact ccggcatgac gggctctcga tgttgtcggt
       61 acgggaaatc gaaaccctcc cacagcagcg atgggtcgtg gcagacaaag gcagagtatc
      121 cgatgagatg gcccttacag atcggttgaa cctggacgcc cgaagtcgtt agctggagag
      181 gagtgtcgtc agactcgcgt cacgccttgg cgacctcctg cgagcgccgc tgacacccga
      241 gatcagtgtc cagcaggtgg agaaggccga gggccgatcg gtaccgcgag caacggacac
      301 ggagcttgtc gcagcacacg ctccgtcgtg ctgccgcgca gggcgtcgag gaagccgtgg
      361 cggccctccg ttgccatgac gatgagatca gcggcagact cgcgtgctac agcggctatc
      421 gcgtcggcca catcaccctc tcgcgtgatc ctctgccagg tccagccctc gtgctgctcg
      481 aaggacaggg cgggtgcatc gcccgcctcg cccacgtaca tgacggtaaa cgtcacctcg
      541 tcacactgaa gtccccaggc tgcccggcag gcggcctcga tcgccgcgcc cggcctgggt
      601 tcctgtgcca ccggtatgag gatattccgc agcgaaaccc cgccatcgcc ccgtgacacg
      661 aagccctcaa gcccccaggg gatgaagagc gtcattagcc cggactggcg tgcgataggc
      721 tgcccgaccg atttcgtaag ccaccgcgcg cggccctcgc gttgatgggt agccagtacc
      781 acgaggtcgg tcggattctg ctcgagataa cccagcgcgg ccgctacggg atccgaatcc
      841 cgggccagca ccttcttgac ctggatccca agcgaggaca cggcgtcccg tggacttccc
      901 ggtggtagca cccgccaccg ctccagcatt tgccgaacgc gcgggaactc cgaccacggg
      961 cgattccccc gctccgaaac gtggaggata ttgaggtcgg cccccgcgac cagcgcgatc
     1021 ttgagtgcat gggcgaaggc gacctcgctg ctctcggaga agtctgaggg atggaagatg
     1081 ctccgaacct ggcgcggaag cggcgcaccg ggaacagtca tgcctctttc ctcggaatgt
     1141 cgtgcctcat taactctgcc ctctcagcga ctccccgaag caaaccgtcc ccgcgtcctc
     1201 cacgactttc gctcgcgagg gcggctgacc ggcgagcgag atacagccat ggtgaaaggg
     1261 agtgcagctt acgccatgct accgccacgt tacagcgccg gaaactgtca gttgtgacag
     1321 gttgcagccg catttctcaa tcattactaa agccgttacc caaacgggca atggcttcgc
     1381 tcctttcagc ccttggaaca caaggaagcg ccccacgcga acggcatgcg gcgctttgct
     1441 atgcccgagg cgagagtcga actcgcacgg ggctgccccc ggtggatttt gagacctacc
     1501 ccctcttaga gcatcaacgc ccactggaac catcgctaac cgcttcaaca ggttctctgc
     1561 tctctcaccg atccgccctg cgcggtgttt cacggcgttt tggtgtggtc acggcaaacg
     1621 aacggcaaag tggttgggga gagtgtggct tcctagcgat gtcaccggtc gctgcacttg
     1681 ggtttcactc cgccactcgc gttgggagca accgcgcgga gtttagtatg aagaccagct
     1741 ccgaggagac gtggatgaag gcagcgagca gtggatttag cagacccatc gcggcgagac
     1801 cgatgccgac gccgtccaca gcgagggtgc ctacgaaatt ctgcaggatc gtccggcgcg
     1861 tccgccgtgc gaggcgaacg gtctcgacga acttgagcag gttgttcccc agcagcacca
     1921 cgctggcact ctcgcgggcg acgtctgtcc cagagcccat cgcgacgccc acattcgcct
     1981 gcatcaaggc gggagcgtcg ttgataccat cgccgaccat ggcggtcacc cgcccgcgct
     2041 cgaccaggct cttgacccgc gcctgcttag cttcgggcag caattcggcg gccagctcgt
     2101 cgacgccgag ctccgcggcc acggcctcgg ctacgttcct cgcatcaccg gtgaggagta
     2161 tggtccggat gccgatcgcc ttgaggccac cgacggccgc cttcgcttcc gggcgaaccg
     2221 agtccgcgac gcggatcgcc cccaggtagc gcccgcctcg ggcgatcagt accgcagaac
     2281 ttccgcgact gtcgtcgctg gcatcctccg gcgctgcgac ctgccgctcg cccaggagtg
     2341 cccgagtgcc cgctacgatc tcgtccccgt caacgcgtgc cgtgatgcct cgcccggggg
     2401 tatacgcgaa gtcctggggc tcgaccacgg atatggaatg cgactccgcg atggcgagga
     2461 tcgctttggc aaacggatgc tcggagcggc gctcggccgc cgcagccgcc tcgagcaggg
     2521 ttcgctccga gatgcccgcc gcgggcagca cctcctccac ttcgggagcg ccgagggtga
     2581 gggtcccggt cttgtcgaag atcacggtgt ccacccgggc gagcgtctcg aggtaccgcc
     2641 cgcccttgat gatcgcgcct tgcctcgcgg cccgaccgat gccgccgagg atggcaagcg
     2701 gggtgcccgc agcgatcccg caggcaccgg cgacaatgac gaccgaaatc gtcgagcgca
     2761 cgtcgcgcgt aatgaggaag gtgagcgcgg ccgcgccaag ggcgaagtag acgagatagc
     2821 ctgcaaggcg atccgcgagc ttctggatgg gtgctcgcga gcgctcagcc cgctccacgg
     2881 cttcgacgat tttcccgaaa ctcgtatccc ggccgagccg ctcggcccgg acctcgaggg
     2941 cacccgattg gttgatcgtc ccagcataag cccaggcccc ggccgacttc tcgaccggca
     3001 tggactcccc cgtgattggc gcctggtcga cgaatgagtg gccgccggcg acggtcccgt
     3061 ccaccggaat ccgactgccg gggttcacca gtactaagtc gccgatccgg atctcgtctg
     3121 cccgcacctg tcggatctca ccgccgtccc gcaccgcggc ggcccggggg aggaagtcca
     3181 gcaggtcacc tatcgctcgc cgcccccggc ggacggtcat cccttccaga atctccgctc
     3241 cgagcacgaa gagcgtgata atgagcgcag tgaagaattc cccgatcagg agcgctgcga
     3301 ggagagcgat ggtcatggaa agctccatcg tcatcttgcg ctccacgacg ttctcggcgg
     3361 cctccctgaa gattggatag ccgccgacca gcgtggccac cagaccgatc acgctgaccc
     3421 gggcgaatgg ttcccagagg tggaaccaca ctgcggccgc ggcaatggcc acgaacccca
     3481 ctcgtgccaa gtcggctttc ttcacgccgt cactc
//
LOCUS       DASRJQ010000223         4918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2817341, whole genome shotgun sequence.
ACCESSION   DASRJQ010000223 DASRJQ010000000
VERSION     DASRJQ010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2817341"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VFB89_04995"
     CDS             <1..291
                     /locus_tag="VFB89_04995"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HZM26688.1"
                     /translation="QWQRSHVNSACKLLLLTHAFESLGCKVVGLRTDNFNFASQRAIV
                     ALGARFDGAIRHHMARRDGTVRDTMMYSILAAEWPDVKRHLALRLARHPGPG"
     gene            312..2165
                     /locus_tag="VFB89_05000"
     CDS             312..2165
                     /locus_tag="VFB89_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZM26689.1"
                     /translation="MCRVASDSRVLAVFLLAAAGAVRTASAQPAVPRYERADCPFPPG
                     AWASSVRLECGYLVVYRNRERPSAGTFRLAVAILHPLTPSTDPPLVLPHGGPSGPGGL
                     RGGEMSFASRNAPILKRDIIVYDLRGAGFSEPALCPETVQQGERGRNEPDRARRQALW
                     NAGARACVDNLTAHGIDPGAFNTTANAADLIDLRKTLGYRQWDAMGVSYGSRLVQEIM
                     RRDPDGVRSAILDSPVIPGPGQQAEVPVAFQRVLDHTFVSCAAQPTCAAAFPTLAKDF
                     YELYDELNATPVEVTYALAETTTVRFDGERLIRALHNGFGTRASRTPLFIHELKHGDR
                     VRTARLLLGNSAVARNSNVLTDLVGCYEGGGAAYQQHLASAALEVRAPFRPLLNTAEE
                     CPIWQERFATPAEREFVRSDIPTLILTKEFDDRTPTDYGRRIAAGLTHVQLLELRGVA
                     HGQSSECQRQIVLSFLANPARALDVSCAEAIPPLSFETRNLEPRSLVMVIASTGPAPS
                     PFAGRWEAVFGGAPVIYRFDLQITGQDLTGVINVAAQAHPIFDGHIDGQTISFRANSP
                     DGGRTVTFTGSLRSGELLFTRTVQVLPGGNPGGVGLFGASGPSTFTAIKVN"
     gene            2897..3565
                     /locus_tag="VFB89_05005"
     CDS             2897..3565
                     /locus_tag="VFB89_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26690.1"
                     /translation="MDRRVLSALPMTGVLSGTALLALATIWYPGGYRWGEHTISALFQ
                     PATPNGVVNPARPLAVLGVLTAMSGIALLFHLVASRTGSAFHKQAIQIGGVASTAFAT
                     LTVTLLHDLMVGLALACFVVALSAVLHMLYRERMLGLFALGVLFVALELGTAVLYFGQ
                     VFLDFLPAGQKATLVLIGLWLFAVQHRSGVSGKPSTWTCPQTSGLPPNTPLQPPSGGK
                     TAAE"
     gene            4219..4848
                     /locus_tag="VFB89_05010"
     CDS             4219..4848
                     /locus_tag="VFB89_05010"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZM26691.1"
                     /translation="MLSHDVTGRLSTALAGRYRVLRQLGEGGMAIVYLAEDVKHQREV
                     ALKLLRPELAPELGTERFLREIRLTAQLVHPNILPVLDSGEADGLLFYVMPLVRGATL
                     RVRLERERQLPVDTAIRITEEVASALSHAHAHGILHRDVKPENILFADDHALLADFGI
                     ARPDAYLANDVKTVSGVALGTPAYMSPEQSAPDGSVDARSDLYALASVT"
BASE COUNT          821 a         1621 c         1558 g          918 t
ORIGIN      
        1 cagtggcagc ggagccatgt caactccgcc tgcaaactgc tgctgctgac ccatgccttc
       61 gaatcgctgg ggtgcaaggt cgtcgggttg cgcaccgaca acttcaactt tgcctcgcag
      121 cgggccatcg ttgcccttgg cgcccgtttc gacggagcga tccgccatca catggcgcgg
      181 agggatggaa ccgtccgcga caccatgatg tacagcatcc ttgccgccga gtggcccgat
      241 gtgaagcggc acctcgcgtt gcggctggcg cggcacccgg ggcccggttg actgcgcctc
      301 cggaggcctc catgtgtcgc gtcgcgagcg attcccgcgt cctcgccgtt ttccttctcg
      361 ccgcggcagg ggcggttcgc accgcctcgg cccaaccggc agtcccacgt tacgagcgcg
      421 ccgattgccc cttcccgccc ggcgcctggg cgagctcggt gcggctcgaa tgcggctacc
      481 tcgtggtata tcggaatcgc gaacgaccct cggccggaac cttccggctg gctgtcgcga
      541 ttctccaccc cctgacaccc tcaacggacc cgccgttggt cctcccgcat ggagggccca
      601 gtgggcccgg cggcctgcgg gggggggaaa tgagcttcgc gtcgcgaaac gcccccatcc
      661 tcaagcggga catcattgtc tacgacctga gaggcgcggg gttttccgag ccagcgcttt
      721 gccccgagac ggttcagcag ggcgagcggg gtcgcaatga accggaccgc gcacgtcgtc
      781 aggccctgtg gaacgccggc gcacgggcat gcgtggataa cctcacggct catggcatcg
      841 atccgggcgc cttcaatacg actgccaacg ctgcggacct gatcgatttg cgcaagacgc
      901 tcggataccg gcagtgggat gccatgggcg tctcctacgg gtcacggctg gtgcaggaaa
      961 tcatgcgccg cgatccggac ggcgttcgga gtgcgattct cgacagcccg gtcattcccg
     1021 gcccgggaca gcaggccgag gtgccggtcg catttcagcg agtgctcgac cacacattcg
     1081 tctcctgcgc cgcccagcca acgtgcgccg ccgcatttcc cactcttgcc aaggatttct
     1141 acgagttgta cgacgagctc aacgccacgc cggtagaagt gacctacgca ctggccgaga
     1201 ccaccacggt ccggtttgac ggcgagcggc tgattcgcgc gctgcacaat ggattcggga
     1261 cgcgcgccag ccgcaccccg ctcttcattc atgaattgaa acacggcgat cgggtccgca
     1321 ccgcgcggct gttgctcggc aacagcgcgg tcgcccggaa cagcaacgtg ctgaccgacc
     1381 tggtgggctg ttatgagggc ggcggggcgg cataccagca gcacctcgcg tccgccgcac
     1441 tggaggttcg tgcgccgttc cggcccctcc tcaacaccgc cgaggagtgc cccatctggc
     1501 aggagcgatt cgccacaccg gcagaacgag aattcgtgcg cagcgacatt cccacgttga
     1561 ttctcaccaa ggagttcgac gatcggacgc ccacggacta cggccggcgc atcgccgcgg
     1621 gcttgaccca tgtccagctg ctcgaattgc gtggtgtggc tcacggccag tcatcggaat
     1681 gtcagcggca aatcgtgctg agtttccttg cgaaccccgc gcgggcgctg gatgtctcgt
     1741 gcgccgaggc cataccgccg ctctcgttcg agaccaggaa tctcgaacca cggagtctgg
     1801 tgatggtcat cgcgtcgacg gggcctgcac ccagcccgtt cgccgggcgc tgggaagccg
     1861 tgttcggcgg cgccccggtg atctatcggt tcgacctcca gatcaccggc caggacctca
     1921 ccggcgtgat taacgtagct gcgcaggcgc atcccatctt cgacggccac atcgatggac
     1981 agaccatcag tttcagggca aactccccgg acgggggacg aacggtgacc ttcactggca
     2041 gcctgaggag cggggagctc ctgtttacgc ggaccgtcca ggtcctgcct ggagggaatc
     2101 ccggaggtgt tgggctgttt ggcgcgagcg gacccagcac attcaccgca atcaaggtca
     2161 actgatcggc agccttagca gcgcttgctg cctggcgagg gcgccttagc aaccgccggg
     2221 tatctttggg ctggttggcg gcggcctcgg caggcccccg cagcagaagc gcgatccgtt
     2281 aggcggccca acgaggtcca gcggtgacac tagccgttct cggcgttctc ctagtcgttc
     2341 tcgtagctgc tctgtcgtac cgccacgctc agcggcggcg cgcgcgagaa gcgatcgagg
     2401 actctaactg gcagtgggag cgcgtccgcc gtagagtcga cgaattgctc gacggccggt
     2461 gggactcgac actagtccta attacgcgat accaagtctc cagtgagtcc gcaggacaac
     2521 tccttcgtca gcggctctcg gagcgctcgt ccgacagagt ggaagtcgta tgggaggagg
     2581 cgccgcacaa ggatcctcgc tgggtcgtgg tcacaacaac cgaaacgacc ctcgtcaccc
     2641 cgatcctggg gcgtcagtgg ctcgcccgta tgcgaacggt ctgtgaatgg ggagcctgtg
     2701 acatggcaca ttggggcgtg ctttggggtg ggtcgcctcc gagcccggcg cccgtcggat
     2761 cggagccagg ctgagcgggc cgcctaacca gcgctcgctg ctgacggtcc gcggcggccg
     2821 gccttccaat gattagctta tatgacgggt ccgccgcgcc ccgcagcaga agcgcgagtc
     2881 gttaggcgga tcaaccatgg atcgacgggt tctcagcgcc ttgccgatga cgggcgttct
     2941 ttccgggacc gcgttgttag ccctcgcaac gatttggtat ccaggcggct atcgctgggg
     3001 cgaacacacg atctccgcgc tgtttcaacc ggcaacgcca aatggcgtgg tgaacccggc
     3061 aaggccactc gcggtgctgg gcgttctcac ggccatgagc ggtatcgcgt tgctgttcca
     3121 cctcgtggcg agtcggactg ggagcgcgtt ccacaagcag gcgatccaga ttggtggcgt
     3181 tgcttcaacg gcgttcgcga cgctcacggt gactctgctt catgatctga tggtcggatt
     3241 ggccctcgcg tgctttgtgg tcgccctcag cgctgtactc cacatgctct acagggaacg
     3301 catgctcggc ctgtttgcgc tgggcgttct attcgttgcc cttgagctcg gcaccgcagt
     3361 cctttacttc gggcaggtct tcctcgattt tcttcccgca gggcagaagg cgacactggt
     3421 tctcatcggg ttgtggctct tcgcggtcca acacagatcc ggggtgtcgg gcaagcccag
     3481 cacgtggact tgtccgcaga catctggcct gccgcctaac acgccgctcc agccgccgag
     3541 cggcggaaag accgcggcag agtgagcatc cagttcgccg ctcgcggctg agcggcgggt
     3601 cgttagaccg tatcgggagt ccgactgtcg accttcaagc ctcagacgcc ggccgaggct
     3661 cgcgaactag atgagcgcct gcgcgagtac gcccgaaccc ttcaggctcc gctccttgcg
     3721 catctgagcc tggaacgtgt cttcacggtt gcgaggacat accccgaacg cgccggaatt
     3781 tgttcctact gcgtcgagat ccagatccac gtcctgtttc tacagaccga tctcgcaaat
     3841 atcgctcggg ctcgcgagac gctcggtggc ccatcctcgg ccggcgaaga ctttgcggtt
     3901 cttgcgggcc ttctgtgcgc caactcggac tttgtcctac gatatcgggc gctgtgggac
     3961 aagctgatgg gttttatagt cctgctcacg aatccagcgg agtttcaggc cttcgcggag
     4021 gcgaaaagcc gcaaaaaggc cttccttggc attgttgaaa ggaaggggaa cttcccgaca
     4081 tccctgtacc agccgccgtc gcgttgggtg ccctgacatc cggggaggga tacatttcga
     4141 tgtggctcat ctcggttccc ccatgccgat taccctgacc accgtctatc gacaggtgcc
     4201 tgcggggttt gtcgccaagt gctgagtcac gacgtcactg gacgcctgtc gaccgcgctc
     4261 gccggccgct atcgcgtact acgacagctc ggcgagggcg gcatggccat cgtctatctc
     4321 gccgaggacg tgaagcatca acgggaggtc gccctcaagc tgctgcggcc ggagctggcc
     4381 cccgaactcg gcactgaacg gttccttcgt gaaatccggc tgacggcgca actcgtacat
     4441 cccaacatcc tccccgttct cgactccggc gaagccgacg gactgctctt ctacgtgatg
     4501 ccgctggtcc gcggagcaac gctccgcgtc cgactggagc gcgagcgcca gctgccggtc
     4561 gacacagcga tcaggatcac cgaggaagtc gcatccgcgt tatcgcacgc gcacgcgcac
     4621 gggatcctgc atcgcgacgt caagccggag aacatcctgt ttgccgatga ccacgcgctg
     4681 ctcgcggatt tcggcattgc ccgccctgac gcgtatctcg ccaacgatgt gaagacggtg
     4741 agtggcgtgg cgttaggcac gccggcctac atgagcccgg agcaatctgc ccccgatggg
     4801 tctgtcgatg cgcggagcga cctgtacgcc ctcgcctccg tgacgtagga gatgctggcg
     4861 ggggagcaac ccttcaccgg gccaaccgcg caggcgattc tcgtccgtcg actcacgg
//
LOCUS       DASRJQ010000224         4372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3733539, whole genome shotgun sequence.
ACCESSION   DASRJQ010000224 DASRJQ010000000
VERSION     DASRJQ010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3733539"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VFB89_05015"
     CDS             <1..426
                     /locus_tag="VFB89_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZM26692.1"
                     /translation="VGWFVQPTLLQASDPGYRLMCEEIFGPVLTLHVYPEARWQETLA
                     LVDRTSPYALTGAVFAQDRAALVEADRALRNAAGNYYVNDKPTGAVVGQQPFGGARAS
                     GTNDKAGSILNLMRWISPRTIKETFDPPTEWRYPFMQES"
     gene            429..2711
                     /locus_tag="VFB89_05020"
     CDS             429..2711
                     /locus_tag="VFB89_05020"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZM26693.1"
                     /translation="MRVALALALSVSALAGPLAAQNEVVKGAVVDSTGAPVGRADLGL
                     PELGRRALSDADGQFTLGPVPPGQYTLVASSPGFRPFAQRIRVPLSEPVVLRLLPQTA
                     TLLAPVTVTATRDPIDPRDSPLPASELSGDALRRSISVSLARAVEELPGLRTLSTGEQ
                     IGKPVIRGLAGSRVLVLENGLRLEDYSWSDEDGPSVDARLAERVEVIRGPASVLYGSD
                     AVGGVINVLPADLPRADPGRSVSTFRTELFGATNNTEGGALLGFEGARGAWGARATIL
                     GRTAGDLHTPEGELGNTGFSALNGELAAGVSGTSGGFFLRYTRYGGEFKLLEAGGPGA
                     NGEEAQADGGEEGGPERKLSDDRIQLDATRVFGGMRLEVKAQGQRHWIAENSDEAPAP
                     DGGTGEGRVFDLVLKTGTLDVLLHHTIGPRVSGTVGLSGMLQDNDSRGPEALVPDART
                     KDGAVFGLERMDLGRWSILAGARVDVRGIDADATPELALPATSRDYTAVTGDLGAVFR
                     LTPTLVLTANAGRAWRAPNLFELFANGPRLGEARFDIGDTTLVPEKSFNLDVGVRWEG
                     RPVRVELAAFRNRIDDFIFGSPTTEFEDSLRIFRYGQTDATLIGGEVRAISDITPRLA
                     AGGQVEYVHGDDAEDRPLPDIPPVRGILEAELHDLALGWFRRTKFGVELELVGAQNRV
                     PTYQLSGSGASVFDTPTDGYALLDVEAATEHMVAGHRLTIAARVQNLTNTEYRDYLSR
                     YKTFALNPGRNFSLRLGWEL"
     gene            2847..3473
                     /locus_tag="VFB89_05025"
     CDS             2847..3473
                     /locus_tag="VFB89_05025"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HZM26694.1"
                     /translation="MWKWMAVAGLCSIAAVSSVEGQAELSSAPARRQVLNAVVTAGRL
                     SFDGRASVGDFTGTTTTVSGELIGGEEITLVRGWVEAPVQTLVTGNGKRDRDLNKSME
                     SDRYPTIRFELAGVTIRSESGDTAKVDLEGRFLIHGVARDVVLPATVVLGGDYTAIRA
                     DTPLNLKDYNIGGLSKAFGMLRMYPDILVHIDVTFASGTAAPAAGAKG"
     gene            3534..4340
                     /locus_tag="VFB89_05030"
     CDS             3534..4340
                     /locus_tag="VFB89_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26695.1"
                     /translation="MRFAGLIALVGLSGLAGCSSGDNLSPPSGQPEEGSRVGLSPSGE
                     VNSSFQVIKGSGEITATVNQFRDALGQPGNGNTPGQQPKGRREIGWDGVPAALDNNNN
                     FPGDFFKKAGAVLTSLGSGLRNSSNDFADLNPAYGAQFEPFTKPKTFAPTGSPSLDLT
                     FQVAGSDTPASVTGVGIVFSDVDRDGSASIKLFDAKGRNLGRYFAPTRSDANGLSFVG
                     VVFDAAIVARVTITAGEAAIDAGLQDISDGGLRDLVVTDNFLYGEPQALP"
BASE COUNT          755 a         1374 c         1452 g          791 t
ORIGIN      
        1 gtcggctggt tcgtgcagcc caccctgctg caggcaagcg atcctggata ccggctcatg
       61 tgcgaggaga ttttcgggcc ggtcctcacc ctgcacgtgt atccggaagc tcgctggcag
      121 gaaacccttg ccctggtgga tcggaccagc ccctacgcgc tcaccggcgc ggtcttcgca
      181 caggatcggg cggcactggt ggaggccgat cgtgctctgc ggaacgcggc gggcaattac
      241 tatgtgaacg acaaacccac gggcgccgtt gtcggacaac agccgttcgg aggggcgcga
      301 gcgagcggca ccaatgacaa ggccggctcc atcctcaacc tgatgcgctg gatttcgcct
      361 cgaaccatca aggagacctt cgacccgccg acggagtggc gctacccgtt catgcaagag
      421 tcctgagcat gcgggtcgcc cttgctctgg cgttgtccgt cagtgcgctg gccgggccgc
      481 tcgctgcaca gaacgaggta gtcaagggtg ccgtggtcga ttcgaccgga gctccggttg
      541 gtcgcgcgga cctgggactt cccgagctcg gccggcgcgc cttgtccgat gccgacgggc
      601 agttcacgct cggcccggtg cctccgggcc agtacactct ggtagcgagc agtcccgggt
      661 tccggccctt cgcgcaacgc atccgggttc ccctgagcga gcctgtagtc ctccggcttc
      721 tgccgcagac cgcgacgctt cttgcgccgg ttacggtcac cgcgacacgg gatcctatcg
      781 atccgcgcga ctctccgttg ccggcctcgg agctatcggg tgacgcgctc cgccgttcga
      841 tcagcgtctc gctcgcccgc gccgtcgaag agctgcccgg tcttcgcacg ctgagcaccg
      901 gagagcagat cgggaagccg gtcattcgcg gactggctgg atcccgagtt ctggtcctgg
      961 agaacggcct tcgactcgag gactactcct ggagcgacga agacggtccc tcggtcgatg
     1021 ctcgcctggc cgaacgagtc gaggtcattc gcggacccgc cagtgtgctg tatggctcgg
     1081 atgccgtggg cggcgtcatc aatgtgctcc cggctgatct gccacgcgcc gaccccggcc
     1141 gttcagtctc gaccttccgc accgagctgt tcggagccac caacaacacg gaaggtggtg
     1201 cgctgctcgg cttcgaggga gcgcggggtg cctggggagc gcgggctacg atactcggtc
     1261 gcacagcagg ggacctgcac acgcccgagg gtgagctggg aaataccggg ttcagcgcac
     1321 tcaacggcga gcttgccgca ggcgtgtcag ggacttcggg cggatttttc ctccgctaca
     1381 cccgctatgg cggtgagttc aagctgctgg aagccggcgg tcccggggct aacggcgagg
     1441 aagcacaggc ggatggcggg gaagaaggcg ggcccgagcg caagctatcg gacgatcgca
     1501 ttcagctcga cgcaacccgt gtgttcggcg ggatgcgcct cgaggtcaag gctcagggcc
     1561 agcgccactg gatcgcggaa aattccgacg aagcccccgc tccagacgga ggcacagggg
     1621 aagggcgggt cttcgatctc gtgctcaaga ccggcaccct ggatgtgctg cttcatcaca
     1681 cgatcggccc gagggtgagc ggcaccgtcg ggctgtcggg gatgctgcag gacaatgaca
     1741 gccgaggccc ggaggccctg gttcccgacg cacgcacgaa agacggcgcg gtgttcggcc
     1801 tggagcgaat ggacttgggg cggtggagca ttctggctgg tgcgcgggtg gatgtgcgcg
     1861 gcatcgatgc cgacgccact ccggagctag cgctgcccgc aacttcgcgt gactacaccg
     1921 cagtgacggg agatctgggc gcagtattcc gcttgacgcc cacactcgtt ttgaccgcca
     1981 acgccggacg cgcgtggcgg gcgcccaacc tgttcgagtt gtttgccaac ggtccccggc
     2041 tcggcgaagc ccgcttcgat atcggagata cgacgctggt accggagaag agcttcaacc
     2101 tcgatgtggg cgtacgctgg gagggaagac cggtccgggt cgagctggcc gcgtttcgga
     2161 atcgtatcga cgacttcatc ttcggctctc cgaccaccga attcgaagat tcgctccgga
     2221 tctttcgcta cgggcagacc gatgccaccc tgatcggggg cgaggtcagg gctattagcg
     2281 atatcacccc ccgtctggcc gcgggtggac aggtcgagta cgtgcacggc gacgatgccg
     2341 aggaccgacc cttgcccgac attcccccgg tgcgcggtat tctcgaagct gagctgcacg
     2401 atctcgcact tggctggttc cggcgaacca agtttggcgt cgagcttgag ttggtcgggg
     2461 cccagaatcg agtaccgacc tatcagctct ccggatctgg cgcttccgtc ttcgacacgc
     2521 caacggacgg gtatgccctg ctcgacgtcg aggcggccac cgagcacatg gtcgctggac
     2581 atcggttgac catcgctgcc cgggtccaaa acctcaccaa taccgaatat cgggattacc
     2641 tgagccgata taagaccttt gcgctcaacc ccggccgcaa tttctccctg cgcctcggct
     2701 gggagcttta accacccggc ccggtgtcag ccgggcgcct acaacgggca tcgtcaactt
     2761 ccgagcgcag gcaacaccgc ccgccgataa cggcgggcgg ttgcgtttcg tactcttgag
     2821 ccgtactcac gaaaccggag caagagatgt ggaagtggat ggctgtcgct gggttgtgct
     2881 cgattgctgc agtctcctcc gtcgaagggc aggccgagct gtcctccgca ccggcccggc
     2941 ggcaagtcct gaacgccgtg gtcaccgcgg gtcgcctgag cttcgacggc cgggccagtg
     3001 tcggcgattt caccggcacc acgaccacgg tgagcggcga attgatcggt ggcgaggaaa
     3061 tcactctggt gcgcggttgg gtcgaggcgc cggtacagac gcttgtgacc ggcaacggca
     3121 agcgggaccg cgatctcaac aagtccatgg agtcggaccg ctatcctacc atccggttcg
     3181 agcttgccgg cgtgacaatc cggtccgagt caggagatac cgccaaggtg gatctggagg
     3241 gccggttcct gattcatggc gtagcccgcg atgtcgtgtt gccggcgacg gtggttttag
     3301 gtggcgacta caccgcgatc cgggcggaca cgccgctcaa cctcaaggac tacaacatcg
     3361 gtggcctcag caaggcgttt ggaatgttac ggatgtatcc ggacatccta gtgcacatcg
     3421 acgtgacctt cgcttccggg acagctgcgc cggccgctgg ggcgaagggc tgacctgaaa
     3481 tcaattcgcc ggaacttccg gcggtgacgt catagaagac gagaggagcg ggtatgcggt
     3541 tcgcaggatt gatcgcactt gttgggctca gcggattagc gggttgcagt agcggcgata
     3601 acctctcgcc cccttcgggg cagcccgagg aaggttctcg ggtggggttg tcgccgtccg
     3661 gcgaggtcaa ttcctcgttt caggtcatta agggaagcgg ggagattaca gcgaccgtga
     3721 accagttccg cgatgctttg ggccagccgg gcaacggcaa cacgcccgga cagcagccca
     3781 agggccggcg cgagatcggc tgggacggcg tgccggcggc gctcgataac aacaacaatt
     3841 tccctggcga cttcttcaag aaggccggcg cggtgctgac cagtctgggc agtggccttc
     3901 gcaacagcag caacgatttt gcagatctca acccggcgta tggggcgcaa ttcgagccct
     3961 tcaccaagcc gaagactttc gcgccgacgg gaagcccctc gcttgatctg actttccagg
     4021 tagcgggcag cgatactccc gcgtctgtca ccggcgtcgg catcgtgttc tccgacgtcg
     4081 atcgggacgg atccgcttcc atcaagctgt tcgacgccaa ggggcggaat ctcggtcggt
     4141 atttcgcgcc cacccgcagc gatgccaacg ggctgtcgtt tgtcggcgtg gtattcgatg
     4201 cggcgatcgt cgcacgggtt accatcacgg caggagaagc cgccatcgat gccgggctgc
     4261 aggatatctc ggacggaggc ctacgggacc tggtggtgac cgacaacttt ctttatggcg
     4321 aaccgcaggc actgccctga gccggcggcg tcgccctgca tttgcccagg cc
//
LOCUS       DASRJQ010000225         2238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3127246, whole genome shotgun sequence.
ACCESSION   DASRJQ010000225 DASRJQ010000000
VERSION     DASRJQ010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3127246"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1878)
                     /locus_tag="VFB89_05035"
     CDS             complement(<1..1878)
                     /locus_tag="VFB89_05035"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HZM26696.1"
                     /translation="MVTALDGSRTSYRHLVAWGLIASFLVAGLYLYPPFLLVHLDQRV
                     YDVLFRSAQRSQTSGQVVIVDLDERSLARFGRWPWPRSQLARLLEKIEALGAASVGID
                     MIFAEPDETPPSPAQQASATLGSEAKAGPSALTPNDAALAAVLSRGSFVLGYNLRFAT
                     EQGDGSGCFLHPIPVVTLQKPGGRAEVAALFQASAVLCSLPALAAAVAGSGFLNAAPD
                     ADGILRRMPLMIAHNGLVYPSLGLATLLPVLGTRQFVLRTTGDRIESLALEDIVVPLD
                     PRGGLLLHFRGGSQTFPYISAADVLGDQLPEGSLRDRIVFLGTSAPGLGESVATPLDT
                     TFPGVEVHATVADSILRRDFIRRSPAAPAIELALIVAAGPVAALMVALAGVVWGGAML
                     LAIAGGLWVATGWLMGSTGLFVSPVFPVVALAFGFTAVNLASFSLERGRADWTTQRFR
                     RTRQIMLKKLRKRETDLRAAQERAKLGSWELDLATQTGSWSAEMFRLFNCDPACGVPT
                     LAEFLEMVHPDDRHLIEEGVTRAVQTGEPVTQEFRSHPSRGPLRHFNAIVHPTKNTEG
                     RVVHLGGTIQDITALIEAKRALDTAERQAILGRVAASVAHEINNPLLAIKTRLHTLKK
                     TM"
     gene            complement(1872..>2238)
                     /locus_tag="VFB89_05040"
     CDS             complement(1872..>2238)
                     /locus_tag="VFB89_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZM26697.1"
                     /translation="IFGEALAAQPAGPARFVLYFKDDSEELTPESEVLLPEVFRTMRD
                     RGALDISVIGHTDTIGTREYNYQLSLRRARKVADLLVKQGVDRNILDIESHGEDNLLV
                     KTGNQVREPRNRRVEITVW"
BASE COUNT          473 a          690 c          686 g          389 t
ORIGIN      
        1 catagtcttt tttagggtat gcagccgtgt cttgatagcg aggagaggat tgttgatttc
       61 gtgcgcaacg gaggcagcca cccgcccgag aattgcctgt cgttcggcgg tatccaaagc
      121 ccgcttcgct tctatgagag cagtaatatc ttgaatcgtt ccacccaagt ggacaacccg
      181 cccttccgta ttctttgtcg ggtggacaat agcattgaag tgtcgcaagg ggccacgcga
      241 gggatggctg cgaaactctt gggtgaccgg ctcgcccgtt tggacagccc gcgtgactcc
      301 ttcctcaata aggtgacggt catcgggatg caccatctcc agaaattccg caagggtcgg
      361 gactccacac gccgggtcac agttaaacaa gcgaaacatc tccgctgacc acgagcccgt
      421 ctgtgttgcc aggtcgagct cccaactgcc gagcttggcg cgctcttggg cagctcggag
      481 gtctgtttcc cgcttgcgaa gctttttcag catgatttgc cgtgttcgtc tgaaacgttg
      541 ggtcgtccaa tcggcccgcc cgcgctcgag agaaaaactt gccaagttga ccgcggtgaa
      601 gccgaaggca agggcgacaa ccggaaatac cggagagaca aaaagccctg tcgagcccat
      661 cagccagccg gtcgccaccc agagcccacc agcgatggcg agcaacatgg caccacccca
      721 cacgactcca gccagagcaa ccatcagcgc ggcgacggga ccagcggcca ctataagcgc
      781 aagctcgatg gcgggcgccg cgggagatcg ccggatgaag tcacggcgca aaatgctatc
      841 ggccacggtc gcgtggacct ccactcccgg aaatgtcgtg tccaacggcg ttgcaacgct
      901 ctcgccgagc ccgggggcag aggtgccgag aaacacgatc cggtcgcgca gcgatccctc
      961 cggtagctga tcgccgagga cgtcggcagc ggagatgtag ggaaaggtct ggctcccgcc
     1021 acggaagtgc agcagtagcc cgcctctcgg atcgaggggc acaacgatgt cttcgagggc
     1081 cagtgattcg attctgtcgc cagtcgttcg gagaacaaac tgccgggtac caagaacagg
     1141 aagcagggtt gcgaggccga ggctcggata aacgagcccg ttgtgagcaa tcatcagcgg
     1201 catgcgccgg agtatcccgt cagcgtcagg cgcagcattt aagaagccag agccggctac
     1261 cgcggccgcc agggccggca ggctgcagag caccgccgac gcttggaaaa gcgccgcaac
     1321 ttccgccctg ccgccgggtt tctgcagggt gactacagga attggatgga ggaagcagcc
     1381 gctcccgtct ccctgctcgg tggcgaaccg cagattgtac ccgaggacga acgagccacg
     1441 gctgagcacc gcggcgagtg ccgcgtcgtt cggcgtcaaa gcgctcggac ccgccttcgc
     1501 ctcggatccc agtgtcgcgg aggcttgctg cgccggagac ggtggggtct cgtcgggctc
     1561 ggcgaagatc atgtcgatcc cgacgctcgc agcgccgagg gcttcgatct tttccaggag
     1621 ccgcgcgagt tggctccgcg gccacggcca gcgaccaaac cgcgcaagac tccgctcatc
     1681 gagatcgacg atcaccacct gtcccgacgt ctgagagcgc tgcgcagacc ggaacaggac
     1741 atcatagacc cgctgatcca ggtggactag gaggaacggc ggatacagat agagcccagc
     1801 gaccaggaag gaggcgatga gcccccacgc aacaagatgc ctgtaggagg tccggcttcc
     1861 atcgagcgcc gtcaccatac ggtgatctca acccggcgat tgcgaggctc ccgaacctga
     1921 ttgccggtct tgacgaggag gttatcctca ccgtgcgact cgatatcgag gatatttcgg
     1981 tccacgccct gcttaactag caggtcagcg acctttctgg cgcgccgtaa tgacagctgg
     2041 taattgtact cgcgtgtccc tatggtgtcc gtatggccga tgacgctgat atccaacgcg
     2101 ccgcgatccc gcatggtccg gaaaacctcc ggaaggagca cctcagattc tggagtcaat
     2161 tcttccgaat catctttgaa atagaggaca aaacgtgcgg ggcctgcagg ctgcgccgcc
     2221 aaagcctccc cgaagatc
//
LOCUS       DASRJQ010000226         5507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3168434, whole genome shotgun sequence.
ACCESSION   DASRJQ010000226 DASRJQ010000000
VERSION     DASRJQ010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5507
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3168434"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VFB89_05045"
     CDS             <1..182
                     /locus_tag="VFB89_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26698.1"
                     /translation="ADEARWAGLAIAASAWAAVLVPWITQHAGMGTVAHQRRMYLALA
                     AWAVTDVVVILAWAA"
     gene            247..876
                     /locus_tag="VFB89_05050"
     CDS             247..876
                     /locus_tag="VFB89_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26699.1"
                     /translation="MAGVVELSSEYDRTDVPHVVRRSIQLGILEAVFVLLASFITRYL
                     DGIAETIPLAIVVLFGLALVSGLPGAWTRARTIEGIAGAAGIGLGATFVFLLVDVALL
                     QPIGTYTNRWREIGGGTNWWYHPVWWMVGTYLPWMGGWILANQSNQGRLPSIPAMMAG
                     ALAFTAICGIAAILIHFPGARWSLPTFGIAFLPGLALATLFSGIRRRAG"
     gene            880..1179
                     /locus_tag="VFB89_05055"
     CDS             880..1179
                     /locus_tag="VFB89_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26700.1"
                     /translation="MRWVRLLLRIFGWLLTPFLAWAAAFFGAVAGALIAMRVRDPRSG
                     VAVTAAIGAVAGFLTILAWLGVLRRSRQVREALAVQSDGTPDIVSDAPETSDPAA"
     gene            1148..1552
                     /locus_tag="VFB89_05060"
     CDS             1148..1552
                     /locus_tag="VFB89_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26701.1"
                     /translation="MRRKLPIPRLSPLLMLAALGCDKGPPAKEPPAAASEAPGPTSPP
                     DSLVLRLPNGTELWYALAREERDSTGAGCVERTLEIRTPTAKIPVPLLYTRDVPRQVN
                     DSTVSARIWNRCVPGDEYRINLRTGQPVRASK"
     gene            1549..2520
                     /locus_tag="VFB89_05065"
     CDS             1549..2520
                     /locus_tag="VFB89_05065"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HZM26702.1"
                     /translation="MKRLPWVTIGAALALGRPVAAMAQSVGVEVGRFFQGEDWTVWRA
                     GVEEPLLGPLSVTLYGAYLREASDIGERLWGGGIDLALFRESRQGAYALGGLAGGLAK
                     NATDDFWGSWSVGAGYQLLPASFLSLAAEARYQKLTPGGRDGVELSFRIGAVFGGKSQ
                     RSSEAARSPSQPISAAEPVTAVPLSTVGDLSPTATLADSVVATAAATMGTAYRLGGTT
                     TEGFDCSGLIQYAYAQHGVSLPRVSADQARAGEKVDRNLAALAPGDILTFSSTGGPVT
                     HVGLYVGQGRFIHSASRGVQLSLLSPDDPYGKWWYARWVGARRVIGR"
     gene            complement(2531..3586)
                     /locus_tag="VFB89_05070"
     CDS             complement(2531..3586)
                     /locus_tag="VFB89_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26703.1"
                     /translation="MTSLLTSALALTLALQGPATPPSVTVKVDSSRHEVTIVAGPWKL
                     PNMPPLDDHMMMDMGAHDAPVQRFIWPVDGWYRGFKYELVDAQGNVLDRRILHHMIVI
                     NFDRRQLLYPAVERLGGAGSETDDASVPKTIGVPLSKGTNLGFYIMWHNETGKDLDAV
                     YLRYSMLWTPTNQNPRPVSSMPIYMDVNLTVGGSNMFDAPPGKSTKAYEFTLPVTGHL
                     LGVGGHMHDYGSMVQLEDATTGKELARVVAKRDSAGRVLKVSRKLFAVSGEGLKLEEG
                     HRYRVVATYDNPTQKTAPGVMAHMVGLFVPDDMRKWPKLDTADPTLRKDLASLQVPGY
                     GDPDAGSGHEQMQHQQQ"
     gene            3828..4145
                     /locus_tag="VFB89_05075"
     CDS             3828..4145
                     /locus_tag="VFB89_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HZM26704.1"
                     /translation="MATATKAKKDLKIHPLEDRVVVLPADEAEAMRGGLYIPDTAKEK
                     PTQGEVIAVGPGRITDKGERITMDIKVGDTVIYGKYSGTPYTLDGDEYVIIKASDVLA
                     KLG"
     gene            4178..>5507
                     /gene="groL"
                     /locus_tag="VFB89_05080"
     CDS             4178..>5507
                     /gene="groL"
                     /locus_tag="VFB89_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HZM26705.1"
                     /translation="MAAKELLFNTDARAKLKKGVDHLAEAVKVTLGPKGRNVVIDKKF
                     GSPTVTKDGVTVAKEVELADAVENMGAQMVKEVATKTSDLAGDGTTTATVLAQAIYRE
                     GLKNVTAGANPMELKRGIDRAVEAVVENLKAISTPSAGKKEIAQVGTISANNDKEIGS
                     LIADAMEKVGKDGVITVEEAKGLETTLETVDGMQFDRGYLSPYFVTDPEKMEAVLEDP
                     YILIHDKKISAMKELLPILEKVAQTGKPMLLIAEDVEGEALATLVVNKLRGTLKVVAV
                     KAPGFGDRRKEMLRDVAVLTAGQVISEEVGFKLENATLNDLGRAKRIVVDKDNTTIVD
                     GKGKESDISGRINEIKAAIDKSTSDYDKEKLQERLAKLSGGVAVINVGAATETEMKEK
                     KARVEDALHATRAAVEEGIVAGGGVAFLRSQSVLEKIKGTDDEKVGIAIVR"
BASE COUNT         1016 a         1655 c         1838 g          998 t
ORIGIN      
        1 ccgccgatga ggcgcgctgg gcgggcttgg cgattgcggc tagtgcctgg gcggccgtgt
       61 tggtgccttg gattacgcag catgcaggca tgggcactgt agcgcaccag cgacgcatgt
      121 acctggccct cgcggcgtgg gcggtgactg atgtcgtggt gatcctcgcc tgggcagcct
      181 gatggccctt ggcccactct cggtcggtcg ggtattattg tgccgggatc gtcaacggag
      241 cattcgatgg cgggtgtggt ggagctgagc agcgagtacg atcgcacgga cgttcctcac
      301 gtcgtgagac ggtcgattca gctgggcatc ctcgaggcag tattcgtgct cctcgcctca
      361 ttcatcactc gctacctcga tggaattgcc gaaaccattc ccctcgccat tgtggtgctg
      421 ttcgggcttg cgttggtgag cggcctgccg ggggcctgga ccagagcgcg gaccatcgaa
      481 gggatcgccg gcgccgccgg catcggcctg ggcgcgacct tcgtgtttct gctggtggat
      541 gtggccttgc tccagccgat cgggacgtac acgaatcgct ggcgtgagat cggtgggggc
      601 accaattggt ggtatcaccc ggtctggtgg atggtcggca cttatctccc ctggatggga
      661 ggctggatcc tcgccaatca gtcgaaccag ggccggctgc cgtccattcc cgcgatgatg
      721 gccggcgccc tcgccttcac cgctatctgc ggaatcgcag caatcctgat ccacttcccg
      781 ggcgcgcgct ggagcctgcc gacgttcggg atcgcctttc ttccgggact cgccctggcc
      841 actctttttt ccgggatacg gcggcgggcg ggctagacaa tgcgctgggt ccgactgctt
      901 ctcaggatct tcggctggct gctcacacca ttcctcgcgt gggcagctgc tttttttgga
      961 gccgtggcgg gggccttgat cgcaatgcgg gtgcgagatc ctcgatccgg cgtcgccgtt
     1021 accgccgcga tcggagcggt cgccggtttt ctcacgattc tcgcctggct aggcgtgctt
     1081 cgccgctcgc ggcaggttcg agaggccctc gccgtgcaga gcgacggcac tcctgacatc
     1141 gttagcgatg cgccggaaac ttccgatccc gcggcttagc ccgctgctga tgctggcggc
     1201 gctcggctgc gataaggggc ctcccgccaa agaaccgccg gctgcagcgt ccgaagcgcc
     1261 aggaccaacg tcgccgcccg attcactggt cttacgcctg ccgaacggaa ccgagctctg
     1321 gtatgcgctg gcccgcgagg aaagggattc caccggcgca ggctgcgtcg agcgaaccct
     1381 cgagattcgc actccaacgg ccaaaattcc ggtaccgctg ctctataccc gcgatgtgcc
     1441 tcgacaggtc aacgactcta cggtgagtgc ccgcatctgg aatcgctgcg tgcctggcga
     1501 cgagtacagg atcaacctgc gaaccggaca acccgtgcgg gcatcaaagt gaagcggctc
     1561 ccatgggtca cgatcggagc cgctctggct ctgggaaggc cggtcgccgc aatggcgcaa
     1621 agcgtaggcg tcgaggtcgg ccgtttcttt cagggagagg actggacggt ctggcgtgcc
     1681 ggagtggagg aaccgctact gggtccgctt tcagtgacgc tctacggagc atacctgcgg
     1741 gaagcctcgg atatcggaga gcgactctgg ggtggcggga tcgatcttgc cctctttcgc
     1801 gagtctcgac aaggcgcata tgccctgggc gggctggccg gagggttagc gaaaaacgcc
     1861 accgacgact tctggggctc atggtcggtc ggggcaggat accagcttct gcccgcctcc
     1921 tttctttcgc tcgctgccga ggcgcgttac cagaagctca ccccgggagg tcgcgatggt
     1981 gtcgagctgt catttcggat tggcgccgtc ttcggcggca agtcacagag gtcgtcagag
     2041 gccgcgcgtt cgccctcgca acccatctca gctgcagagc cagtcacagc agtaccgctc
     2101 tcgactgtcg gcgatctgag tcccactgcc acgctggctg attcagtagt cgcgaccgcg
     2161 gctgccacga tgggaaccgc ttaccggctg ggcggcacca cgaccgaggg attcgactgc
     2221 tcgggcctga ttcagtacgc ctatgctcaa cacggggtga gcctgccgcg ggtaagcgcc
     2281 gaccaggccc gcgccggcga gaaggtggat cgaaacctcg ccgccctcgc gcccggtgac
     2341 attcttactt tttccagcac gggcggtccg gtaacacacg tcggactcta cgtcgggcaa
     2401 gggcggttta tccacagcgc cagtcggggt gtccagctga gcttactcag tccggacgac
     2461 ccctatggca agtggtggta cgcgcgatgg gtaggagcgc ggcgggttat cggccgctaa
     2521 gcgattcggg tcactgctgc tggtgctgca tctgctcgtg cccgcttcca gcgtcgggat
     2581 ctccgtaacc cggaacctgg agcgacgcca ggtctttgcg gagcgtcggg tccgctgtat
     2641 cgagcttcgg ccacttcctc atgtcgtccg ggacgaagag gccgaccatg tgagccatca
     2701 ctcctggggc ggttttctgg gtcgggttgt cgtaagtcgc caccacgcga tagcggtgcc
     2761 cctcttccag cttcaatcct tctccactca cggcgaagag cttacggctc accttaagta
     2821 ctctcccagc tgaatcgcgc ttggcgacga cgcgagccaa ctccttgccg gtggtcgcat
     2881 cctcgagctg aaccatcgag ccgtagtcgt gcatatggcc accgacgccg agcagatgac
     2941 cggtaaccgg gagggtgaac tcgtaggctt tggtcgattt gcccggtggc gcgtcgaaca
     3001 tgttgctgcc gccaacggtc aggtttacgt ccatgtagat gggcatcgag ctgaccggac
     3061 gcgggttctg gttggtaggc gtccacagca tcgagtagcg gaggtagacg gcgtccagat
     3121 ccttcccggt ctcgttgtgc cacatgatat aaaagccgag gttggtgccc ttcgagagcg
     3181 gtactccgat cgtcttcggc acggaggcat cgtccgtctc cgaacccgcc ccgccaaggc
     3241 gttcgacagc ggggtagagc agctgccggc ggtcaaagtt aatcacgatc atgtgatgca
     3301 ggatccgccg gtcgaggacg ttgccctggg cgtccaccaa ttcgtacttg aaaccgcggt
     3361 accacccatc caccggccag atgaaacgct gcaccggagc gtcatgggcg cccatatcca
     3421 tcatcatgtg gtcatccagg ggcggcatat tgggcagttt ccaggggccg gctacgatcg
     3481 tcacttcgtg acgggaggaa tccaccttca ccgtcaccga tggaggtgtg gctggcccct
     3541 gcagcgcgag cgtcagcgca agagcggagg tgaggagcga ggtcattctg gttcctggct
     3601 tgggccttta ggtccggcac gacggcgaga agattctgcg cggtcgggag gcaagtcaag
     3661 cacgccggtt ttcgggtgct tgacagggga atggggggtt ttatcttatc gcgcgagttt
     3721 ctagcagtca cataccaaga gtgctaatca ctctggagca tgaactgcta ggtaactgca
     3781 ggataggaac ttaccacaga cacccgagct gcaactggag gcacgtgatg gcgacggcga
     3841 cgaaggccaa gaaggacctt aagatccacc ccctcgagga tcgcgtggtc gtcctgccgg
     3901 cggacgaggc cgaggcgatg cgcggcgggc tctacatccc cgacaccgcg aaagagaagc
     3961 ccactcaggg tgaggtcatc gccgtgggac cgggacgcat taccgacaag ggcgagcgga
     4021 tcacgatgga catcaaggtg ggtgacacgg tgatctacgg gaagtacagc ggcacgccct
     4081 acaccctcga tggcgacgag tacgtcatca tcaaggcgtc ggacgtcctc gccaagctcg
     4141 gctgacctca gcccgaatcc accaaggaga aacaccgatg gctgcgaagg aactgctgtt
     4201 caacacggat gcccgggcca agctcaagaa gggcgtcgat catctcgccg aggcggtgaa
     4261 ggttacgctc ggccccaagg gacggaacgt cgtcatcgac aagaagttcg gctcgcccac
     4321 cgtcaccaag gacggcgtca cagtggccaa ggaagtcgag ctggccgacg cggtcgagaa
     4381 catgggcgcg cagatggtga aggaagtcgc caccaagacc agcgacctgg cgggtgacgg
     4441 caccaccacg gcgaccgtgc ttgcgcaggc gatctaccgc gaggggctga agaacgtcac
     4501 cgcgggcgcc aaccccatgg agctcaagcg gggcatcgac cgggcggtcg aggcagtggt
     4561 agagaacctg aaggccatct ccactccgtc ggcgggcaag aaggagatcg cccaggtcgg
     4621 caccatctcc gcgaacaacg acaaggagat cggtagcctc atcgccgatg cgatggagaa
     4681 ggtcggcaag gacggggtga tcacggtcga ggaggcgaag ggcctggaga ccacgctcga
     4741 gaccgtggac ggcatgcagt tcgaccgcgg gtatctctcc ccttacttcg tcaccgatcc
     4801 ggagaagatg gaggcggtgc tggaagaccc ctacatcctg atccacgaca agaagatctc
     4861 ggcgatgaag gagctgctcc ccatcctcga gaaggtggcg cagactggca agccgatgct
     4921 gctgatcgct gaggacgtcg agggagaggc gctggctacg ctggtggtga acaagctgcg
     4981 cggcaccctc aaggtcgtgg cggtcaaggc cccgggtttc ggcgatcgcc gcaaggagat
     5041 gctgcgtgac gtcgccgtgc tcacggccgg ccaggtgatc tcggaggagg tcgggttcaa
     5101 gctggagaat gcgaccctca atgatcttgg gcgcgccaag cgcatcgtgg tggacaagga
     5161 caacaccacc atcgtggacg gcaagggcaa ggagagcgac atctcgggcc ggatcaacga
     5221 gatcaaggct gccatcgaca agagcaccag cgactacgac aaggagaagc tgcaggagcg
     5281 gttggccaag ctctccggcg gcgttgcggt gatcaatgtg ggtgcggcga ccgagaccga
     5341 gatgaaggaa aagaaagctc gggtcgagga tgctctgcat gctacccgcg ccgcggtcga
     5401 ggaaggcatc gtggcgggcg gtggggttgc gttcctccgg agccagtcgg tgctcgagaa
     5461 gatcaagggc acggacgacg agaaggtagg catcgccatc gtgcgtc
//
LOCUS       DASRJQ010000227         4577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3294869, whole genome shotgun sequence.
ACCESSION   DASRJQ010000227 DASRJQ010000000
VERSION     DASRJQ010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3294869"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VFB89_05085"
     CDS             <1..782
                     /locus_tag="VFB89_05085"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HZM26706.1"
                     /translation="RVPVLADLKPSGRYVATDLHRAGGVPLVMKMLLEHGKLHGDALT
                     ITGETVAESLRDVSPTPRADQDVVRPWERPVYPQGHLAILRGNLAPEGSVAKITGIKT
                     HRMTGPARVFDSEEACLDAILAGKIRSGDVLVIRYEGPKGGPGMREMLAPTSAIIGAG
                     LGDSVGLITDGRFSGGTYGMVVGHVAPEAALGGTLALLEEGDTITIDADRRLLQVDLS
                     DATLTRRRAAWKAPTPRYTTGVLHKYAKLVSSASLGAVTDL"
     gene            808..1539
                     /locus_tag="VFB89_05090"
     CDS             808..1539
                     /locus_tag="VFB89_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HZM26707.1"
                     /translation="MIRPVGARPPGRELFVYLPPSYAATDRSYPVLYMQDGQNLFDPA
                     LSYAGSWCVDLALNRAAQRGLEAIAVGVPNLGEERIAEYNPFPGPGSADAQGESYLDY
                     LVGSVKQLVDRTFRTLPGRESTGIVGSSMGGLISLFAFFRRPEIFGIVGALSPSLWFA
                     DRAIFDVAKTAPFRAGKVYLDVGRREGPPTVADTRTLRDLLEAKGYQRDRDLRYVEDK
                     SGTHQESAWRRRFRSAVAFLLSPAA"
     gene            1543..2262
                     /locus_tag="VFB89_05095"
     CDS             1543..2262
                     /locus_tag="VFB89_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HZM26708.1"
                     /translation="MHHEFHRWYSPALGRDMALDVVGHHGARVLVFPTSMGTHREWLD
                     RRMHEVLGDHLNNGWLQLFLLDHLPGETWYAEHTHPGARAWRHLQYDHYLETEVLPFT
                     ASLNSNPFMIATGASLGAYYAACFGLRHPHLVHRILGMSGLYDIKQLTGGYSDHNVYA
                     CNPADFMRHEHDPSRLDAFRRQDIILAVGRDDAARVNNEELSATLWSKGIGNALRIWD
                     GWAHDWPYWERMLRTYIGGHD"
     gene            2336..3271
                     /locus_tag="VFB89_05100"
     CDS             2336..3271
                     /locus_tag="VFB89_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26709.1"
                     /translation="MPPKRVALFVGREWSFPPAFLEEVHRRDAGVVVEYARIGASSMD
                     HPCPYDVIIDRISHEVPYYRTYLKKAVLDGATVVNNPFMWSADDKFFGASLATKLGVA
                     SPKTLALPNKSYIAGIVPNESLRNLEFPLDWQAIAEQIGMPCILKDAHGGGWKEVYVC
                     RSLDELVHHYDNSGLLTMVVQEFIQWEQFVRCLCLGQEDILPMPYDPRERRYLVEPGY
                     LSPELEARIVQDARTLVRALGYDMNSIEFAVRDGIPYAIDFMNPAPDMDIYSLTPTYF
                     EWTVQKMADMAIRLAKEPRRQSQALRWDAVFTVAR"
     gene            3279..>4577
                     /locus_tag="VFB89_05105"
     CDS             3279..>4577
                     /locus_tag="VFB89_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26710.1"
                     /translation="MQDAIAFYHGLLSDELAQASQAMLDDQLRRRGLVFGERPLATVL
                     RPRFMALAHYRRLQRRVRVLMRAFGKAYERAVTNAAFRTQFRLTDWEEQLVRHEPGFH
                     APSPTSRLDAFVLDESGEMALTEYNAETPAGAGYNDALVASFADLPIMRAFGKRYEAL
                     PLVARHGVLHVLLEAYEEWAGRRAIPRVAIVDWPDVPTRSEFVLFREYFEAVGMDTVI
                     ADPRELEFRGGRLYADRGPVDLVYKRVLLSELVERCGLESPLLRAVAARAVCMVNPPR
                     SKILHKKASLAVLSDERNSELFDSEERAAIAAHIPWTRVVEQRQTEHGGRSIDLIPFI
                     LESRTRMVLKPNDDYGGAGIVLGWQTDATGWEQAVATALASPHIAQERVPIPSEPYPS
                     LVDGRAAFADRMLDTAPFVCGGEYAEGCLTRLSTAALLNVT"
BASE COUNT          795 a         1452 c         1476 g          854 t
ORIGIN      
        1 ctcgtgtgcc ggttctggcc gacctcaagc cgtcggggcg gtacgtggcc accgatctgc
       61 accgggctgg cggtgtgccg ctggtgatga agatgttgct cgagcacgga aagctgcacg
      121 gtgacgccct cacgatcacg ggcgagaccg tggccgaatc gctgcgcgac gtttctccaa
      181 cccctcgcgc agatcaggat gtggttcgcc cgtgggagcg gccggtctac ccgcaggggc
      241 acctggcgat cctgcgcggc aacctcgccc ccgagggctc ggttgccaaa atcaccggca
      301 tcaagaccca ccgcatgacc ggaccagcac gggtctttga ttcagaggag gcgtgcctcg
      361 atgcgatcct ggcaggaaag atccgctccg gagacgtgct ggttatccgc tacgagggcc
      421 ccaaaggggg acccgggatg cgggaaatgc tcgcccccac ctcagcgatc atcggggccg
      481 gcttgggcga ctcggtcggt ctcatcacgg atggccgctt ttccggcggt acctacggga
      541 tggtcgtggg tcacgtcgca ccagaagctg cgttgggcgg aaccctggcg ttgctggagg
      601 agggcgacac cattacgatc gacgccgacc ggcgcctact tcaggtcgac ttgagtgacg
      661 ccactctcac gcggcgacgg gcagcctgga aagcgccgac tccccgatac accacgggcg
      721 tcctccacaa gtacgccaag ctcgtctcca gtgcgagcct tggagcggtc accgatctgt
      781 agcgagcgac gtcgaggccg gatcgaagtg atccgtcccg ttggggcgcg accgccgggg
      841 cgggaactgt tcgtgtacct tcccccgtcc tatgccgcga ctgaccggtc ctaccctgtc
      901 ctgtacatgc aggatggcca gaatctcttc gaccccgcgt tgagctacgc ggggtcctgg
      961 tgtgtggatc tcgccctgaa ccgggcggca cagcgcggac tcgaagccat cgcggtaggt
     1021 gtgccgaact taggagagga gcgaatcgcc gagtacaatc cgtttcctgg gccgggaagc
     1081 gcagacgccc aaggcgaatc ctatctggac tatctcgtgg ggtcggttaa gcagctcgta
     1141 gacaggacct ttcgcacttt gccggggcga gagtcgactg gcatcgtcgg ctcgtcgatg
     1201 ggagggctca tcagtttgtt cgccttcttc cgtcggcccg agatattcgg catcgttggg
     1261 gcgctgagcc catcattgtg gttcgcggat cgagctatct tcgacgtcgc gaagacggcg
     1321 ccctttcgag ctgggaaggt ctatctcgat gtgggccggc gcgagggccc gcccaccgtg
     1381 gccgatacgc gaacgctccg agacttgctc gaggcaaagg ggtatcagcg ggatcgggac
     1441 ctgcggtacg tggaggataa gtcgggtacc caccaggagt cggcttggcg ccggcgtttc
     1501 cggtcggcgg ttgcgttcct cctttcacct gctgcctaga cggtgcatca cgaatttcac
     1561 cgctggtaca gtccggcctt gggccgggac atggcgctgg acgtggtcgg ccaccacggc
     1621 gctcgggtgc tggtctttcc tacatcgatg ggaacccatc gcgagtggct cgaccgccgg
     1681 atgcacgagg tcctcggcga ccatctgaac aacggttggc tccaactctt cctgctggac
     1741 caccttcccg gagaaacctg gtatgccgag cacactcacc ccggcgccag agcctggcgg
     1801 cacctgcaat acgatcacta cctggagacc gaggtcctgc ctttcacggc cagcctgaac
     1861 tcgaatccgt tcatgattgc tacgggggcg agtctggggg cttattatgc cgcctgtttc
     1921 gggctacgcc acccgcatct ggtccaccgg attctgggga tgagtgggct ctacgacatc
     1981 aagcagctga cgggcgggta ttcggaccac aacgtctatg cctgcaatcc ggcggatttc
     2041 atgcgacacg agcacgatcc gtcgagactt gacgcgttcc gccgtcagga catcatcctc
     2101 gcggtcggac gcgacgatgc cgcccgagtc aacaacgagg aactttcggc caccctctgg
     2161 agcaagggga ttggcaatgc cttgcgcatc tgggacggct gggctcacga ttggccgtac
     2221 tgggagcgga tgctgcggac ctacatcggg ggccacgatt gaataccctt tcggagccac
     2281 ttctgcgatc cacggcagat aggtgctagc tgatagctga taccggatta tgcccatgcc
     2341 ccccaaacgc gtcgctttgt ttgtcggccg ggagtggtcc tttcctccgg cctttttaga
     2401 ggaggtccat cgccgagatg ccggcgtcgt cgtggagtac gcgcggatcg gcgccagctc
     2461 gatggaccat ccctgcccgt acgatgtgat catagatcgg atctcccatg aggtgccgta
     2521 ctaccgcacc tatctcaaga aggccgtgct cgacggcgcc acggtcgtca acaatccgtt
     2581 catgtggagt gccgacgaca agttcttcgg ggcaagcctg gcgacgaagt tgggagtcgc
     2641 ttcccccaag acgctcgcgc ttccgaacaa gtcctacatt gccgggatcg tgcccaacga
     2701 gagcctgcgc aacctggagt ttccgctcga ttggcaggcc atcgccgagc aaataggaat
     2761 gccctgcatc ctcaaggatg ctcacggcgg tggctggaaa gaggtctacg tgtgtcgctc
     2821 gctcgacgag ctggtccacc actacgacaa ctccggactc ctgaccatgg tggtgcagga
     2881 attcatccag tgggagcagt tcgtccgctg cctgtgcctg gggcaggagg acatcctgcc
     2941 gatgccctat gatccccggg agaggcgtta cctcgtagag ccggggtacc tcagccctga
     3001 gctcgaggct cgcatcgtcc aggacgctcg cactctggtc cgcgcgctgg gttatgacat
     3061 gaactcgatc gagtttgccg tgcgtgatgg cattccatat gcgatcgact tcatgaaccc
     3121 agcccctgac atggacatct attcgctcac accgacctac ttcgagtgga ccgtgcagaa
     3181 gatggccgac atggcgatcc gcctggcgaa ggaaccgcgc cggcagtcgc aggcgctccg
     3241 gtgggacgcg gtgttcaccg tggctcggta acccaggcgt gcaggacgcc atcgcctttt
     3301 accacggtct gctttccgac gagttggccc aggcgtcgca ggcgatgctc gacgaccagc
     3361 tgcgccgccg gggcctggtg ttcggagagc ggccgctggc aaccgtgctc cgtcctcgct
     3421 tcatggcgct ggcgcactac cggcggcttc agcgccgggt gcgtgttctt atgcgcgcct
     3481 tcggaaaagc ctatgagcgt gcagtcacga acgctgcctt ccgaacccag ttccggctca
     3541 ctgactggga agagcagctg gtccggcacg agcccggctt ccatgcgccc agcccgacct
     3601 cccggctgga tgccttcgtg ctggacgaga gcggtgagat ggccttgacc gagtacaacg
     3661 ccgagacacc ggcgggggca ggctacaacg acgcgctggt cgcttccttc gcagacctcc
     3721 cgatcatgcg cgctttcgga aagcgatacg aggcactgcc gctggtcgcc cgtcacggcg
     3781 tgctccatgt gctcctggaa gcctacgagg aatgggccgg ccgccgcgcc ataccacggg
     3841 tggcgatagt ggactggccg gacgtgccga cccgcagcga gttcgtgctg ttccgggagt
     3901 attttgaagc ggtggggatg gacacggtca ttgccgatcc ccgcgagttg gagttccgag
     3961 gcggccgcct ctacgctgac cgtggaccgg tggacctggt ctacaagcgc gtcctcctga
     4021 gcgagctggt cgagcgctgc ggtttggaaa gccccctgct ccgggcggtg gccgcaaggg
     4081 cggtttgcat ggtcaacccg ccgcgaagca agatccttca caagaaggcg agcctggccg
     4141 tcttgagcga cgagcgaaac tctgagctgt tcgattcgga ggagcgagcc gcgatcgccg
     4201 cccacattcc ctggacccgg gtggtcgagc aacggcaaac cgagcacggg ggtcggtcga
     4261 tcgatttaat accattcatt ctcgagagcc ggacgcgaat ggtgctcaaa cccaatgacg
     4321 actacggcgg ggctggtatc gtcctcggct ggcagaccga cgccaccggc tgggagcagg
     4381 cagtagccac ggcgctggcg agccctcaca tcgcccagga gcgggtgccg atccccagtg
     4441 agccctatcc cagcctggtc gacggccgcg ccgcctttgc cgaccgcatg ctggacaccg
     4501 cgccattcgt ttgcggaggc gagtacgccg agggatgtct gacgcgcctc tccaccgcgg
     4561 cactgctcaa cgtaacg
//
LOCUS       DASRJQ010000228         3941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3731129, whole genome shotgun sequence.
ACCESSION   DASRJQ010000228 DASRJQ010000000
VERSION     DASRJQ010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3731129"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VFB89_05110"
     CDS             <1..233
                     /locus_tag="VFB89_05110"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZM26711.1"
                     /translation="DDALRGVTLAPAEVFGVQDRYGSLEPGKMANLVVWSGDPFEFSS
                     AAEHIFIRGRDVAPVSRQTQLRDRYRELPPRF"
     gene            237..863
                     /locus_tag="VFB89_05115"
     CDS             237..863
                     /locus_tag="VFB89_05115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HZM26712.1"
                     /translation="MSELARFGLITFTSILFIVDPVAAVPTYLVITQDETPESRRRTA
                     LRACIAMTLLLTVFAATGTLIFRAFGITLPAFRVAGGLILWFSAMEMLRGERPSREGR
                     VEIQEAQEKEDVAIVPLAIPLLAGPGAISTIVVLAGQAHAPPHVALLYAAILVTGIVS
                     YLTLRLGEPLLRRLGRTGIRVITRIMGLLLAAVAVQFVLTGVNEGLRR"
     gene            complement(865..1476)
                     /locus_tag="VFB89_05120"
     CDS             complement(865..1476)
                     /locus_tag="VFB89_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26713.1"
                     /translation="MTESAAGVGPAMLLLGAWHGVNPAMGWLFAVALGFQERRGAAVW
                     RALPPLALGHGLAIAAAVFAAVVAGVVVGPEPLKWVVAAGLLAFGLSRLIRARHPRWV
                     GMRVGFRDLVVWSTLMASAHGAGLMVIPWILSSGTAHGGASEAIGLAATALHTGAYLG
                     VTALAALTVYYWLGVSWLRTLWVNLDLAWGVALIATAVATPFL"
     gene            complement(1473..2876)
                     /locus_tag="VFB89_05125"
     CDS             complement(1473..2876)
                     /locus_tag="VFB89_05125"
                     /inference="COORDINATES: protein motif:HMM:NF017505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding family protein"
                     /protein_id="HZM26714.1"
                     /translation="MARWTPDPTFYASPRDAMQAPAEELAYVALLDPTGRRPDAMGVV
                     DVNPRSTTYASLVGRTDLPNVGDELHHFGWNACSSCLCPSAPHAHLERRYLVVPGINS
                     SRLHILDTKPDPRSPKVTKVIEPEEVAAKTGYASPHTVHCGPDGIYINALGSARTGDG
                     PGGIFVLDHETFELIGAWEKERGPQYLSYDFAWHLAHDTLVTSEWGTPNMVRQGLNPE
                     LLLAGKYGNQLHVWDLRNRRHLQVLALGPEYQITLELRTARDPSKAYGFAGVTISLKD
                     LSASIWLWHRAQGNGSWKVDKVIDIPAEPADPELLPPLLKGFKAVPPLVSDINLSLDD
                     RFLYVSCWGTGQFLQYDVSDPFHPRKTGEVRIGGIVQRTPHPSRPDTPLNGGPQMVEV
                     SRDGRRVYFTNSLYSPWDVQFYPEGIRGWMVKLDAAKEGGLTFDRNFFFDLNDGMRPH
                     QVRLQGGDASSDSFCFA"
     gene            3033..3476
                     /locus_tag="VFB89_05130"
     CDS             3033..3476
                     /locus_tag="VFB89_05130"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZM26715.1"
                     /translation="MSFGLRPLLLAAIVALAAAPKALAQVGGTTDIITGKVTGPSGEP
                     IADATVEVVSVETQVTRDRATDAHGRFTILFPEGGGQYLLLVRYIGMTPLRLSVARQA
                     DEDRLVVNAQTRQGLASAREPLTPEQWAKLPEPVGAPDTRRRRAN"
     gene            3560..>3941
                     /locus_tag="VFB89_05135"
     CDS             3560..>3941
                     /locus_tag="VFB89_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HZM26716.1"
                     /translation="MRRYLTEFIGTFFLVFTIGLTVTAGAPMAPLAIGSSLMIMVYMG
                     GHVSGGHYNPAVSLALLLRGKMESVVEFVAYVLSQLAGAIVAALATYVIVGQTLKVAP
                     GPSASTLGALLVEILFTFALALVVL"
BASE COUNT          704 a         1249 c         1267 g          721 t
ORIGIN      
        1 gggacgacgc gcttcggggt gtgacgctcg cgccggccga ggtgttcggg gtccaggacc
       61 gctatggatc cctagagccg ggaaagatgg ccaatctggt cgtgtggtcc ggcgatccct
      121 tcgagttctc cagcgcggcc gagcacatct tcattcgcgg acgcgatgta gcgcccgtga
      181 gtcgccaaac tcaactgcgg gatcgctatc gcgagttgcc gccccggttc tgaccagtga
      241 gtgaactcgc gcgctttggg ctgatcacct tcacctccat cctcttcatc gtggatccgg
      301 tggcggccgt tcctacgtat ctcgtgatca cgcaggacga aaccccggaa agccgccggc
      361 gcaccgcgct ccgcgcctgc attgcgatga cattgctcct caccgttttc gcggccacgg
      421 gaacgctgat cttccgcgcg ttcggcatta ccctcccggc attccgcgtg gcgggtggcc
      481 tcattctctg gttttccgcc atggaaatgc tgcgaggcga gcggccgagt cgggaggggc
      541 gagtggagat tcaggaagcg caggaaaagg aggatgtcgc gatcgtgccc ctggcgatcc
      601 ccttgcttgc cggtcccggg gccatctcga ccatcgtcgt tctggcaggc caggctcacg
      661 caccgccgca cgtcgcatta ctctatgcgg cgattctcgt gaccggcatc gtttcttatc
      721 tcaccctgcg actgggcgag ccactgctcc gccgtctggg aaggacgggg attcgcgtca
      781 tcactcggat catggggctt ctgctcgccg cagtggcggt gcagttcgta ctgactggcg
      841 taaacgaagg cttgagacgc tgaatcagag aaagggggtc gcgacggcgg tggcgatcag
      901 ggcgacgccc caggccagat cgagattcac ccacaacgtg cggagccagc tcactccaag
      961 ccagtaatag accgtcaagg cggcgagcgc ggtcactccg agatacgccc cggtgtggag
     1021 tgccgtcgcg gcgagcccta tcgcttcaga ggcgccaccg tgtgcagtgc ctgagctcag
     1081 aatccagggg atcaccatga gcccggcgcc gtgtgccgac gccatgagcg tggaccacac
     1141 gaccagatcg cgaaacccca cccgcatacc gacccagcga ggatgacggg cccggatcag
     1201 ccgcgacaat ccaaaggcga gcagaccggc ggctaccacc cacttgagcg gctccggccc
     1261 cacgaccacc cccgcgacta cagctgcaaa cacagcggcg gcgatggcaa gcccgtgtcc
     1321 gagcgcgagc ggcggcaatg cccgccatac ggccgctcct cgccgttcct gaaagccgag
     1381 cgccacggcg aagagccagc ccatggcagg gtttaccccg tgccacgcgc ccagcagaag
     1441 catggctggc ccgacgcccg cggcgctttc ggtcatgcga agcagaaaga atccgaggac
     1501 gcgtcgccgc cctggagccg cacctggtgt ggccgcatcc cgtcgttcag gtcgaagaag
     1561 aagtttcggt cgaaggtaag gccgccctcc tttgcggcgt cgagcttcac catccagccg
     1621 cggatgcctt cgggatagaa ctgcacgtcc cacggcgagt agagtgagtt ggtgaagtag
     1681 acccggcgac cgtcgcggct cacctcgacc atctgggggc cgccattgag cggcgtgtcc
     1741 ggccgggacg gatggggagt acgctgtacg attccaccga tgcgaacttc gccggtcttc
     1801 cgcggatgga atgggtccga gacatcgtat tggagaaact gccccgtgcc ccaacacgag
     1861 acgtacagaa aacggtcgtc gagcgacagg ttgatgtccg acaccagtgg cggcaccgcc
     1921 ttgaatcctt tgagcaaggg aggcagcaac tcgggatcgg ccggctccgc aggaatgtcg
     1981 atcaccttgt cgaccttcca ggagccattg ccctgagccc gatgccagag ccagatcgag
     2041 gcggagagat ccttgagcga gatcgtaacg ccggcaaagc cgtatgcctt gctcgggtcg
     2101 cgagccgtac ggagctcgag cgtgatttga tactccggac ccaacgccaa gacctgcaaa
     2161 tggcgacggt ttcgcaggtc ccagacgtgg agctgattgc cgtactttcc ggccagcagc
     2221 aactccgggt tgagtccctg acggaccatg ttgggtgtgc cccattcact ggtcaccagc
     2281 gtgtcgtgcg ccagatgcca ggcgaaatcg tatgagaggt attgagggcc gcgctccttc
     2341 tcccaggccc cgatcagttc gaaggtctca tggtccagta cgaagatgcc ccccggacca
     2401 tcgcccgtgc gggccgaccc gagcgcgttg atgtaaatgc cgtcgggccc gcaatggacg
     2461 gtatgggggc tggcgtagcc ggtcttcgcg gcgacttcct ccggttcgat caccttcgtc
     2521 actttgggcg aacggggatc gggcttcgta tccaggatgt gcagccgcga cgagttgatt
     2581 cccggcacaa ccagatagcg acgctcgaga tgtgcgtgcg gcgcggacgg gcacaagcag
     2641 gagctgcagg cgttccaacc gaagtgatgc agctcgtccc caacatttgg tagatccgtt
     2701 cggccgacca gcgaggcgta tgtggtcgag cgcggattga cgtccaccac gcccatcgca
     2761 tccggccggc ggccggtcgg atcgagcagg gcgacgtacg ctagttcctc tgccggcgcc
     2821 tgcatggcgt ctcgaggaga agcgtagaaa gtcgggtcgg gggtccagcg ggccatagaa
     2881 agctccagag tccagggggt ggacgagctc aggcggctaa catgggaact gcgacggcgg
     2941 gaaggcaagg ggccctttcg ccgcgtttcg cggcctaaac gtgctggggc cctcgcccgt
     3001 atatcgacaa gatctttcca ggaatcggct tcgtgtcgtt cggcctccgg ccgctgctgc
     3061 tggcagctat cgtggcattg gctgcagcgc cgaaagctct tgcgcaggtc ggtggcacca
     3121 ccgacatcat caccggaaaa gtcacggggc cctccgggga gccgatcgcc gatgcgactg
     3181 tcgaagtcgt gtcggtcgag acgcaggtaa cccgcgaccg ggccactgat gcccacggcc
     3241 gattcaccat cctgttcccc gagggtggtg gtcagtatct gctgctggtg cggtacatcg
     3301 gcatgacacc gttgcgactg tccgtcgctc gccaggccga tgaagatcgt ctcgtggtga
     3361 acgctcagac gcgccaaggg ctcgccagtg ctcgcgaacc gctcacaccg gagcagtggg
     3421 ccaagcttcc ggagcccgta ggcgcgccgg acactcgacg gcggcgagcg aactagcaaa
     3481 cgccattgcg cggtttccgc ggctgagtaa aatcgggacc gcacacgcgt gattccgcca
     3541 tttccccgga gcttttctca tgcgacggta tttgaccgag ttcatcggca cgttttttct
     3601 cgtgtttaca attgggctca ccgtcaccgc aggtgcaccg atggcgccgc tcgcgatcgg
     3661 atcctcgttg atgattatgg tctacatggg tggccatgta tccggtggtc attacaaccc
     3721 tgctgtgtct ctggcgctgc tgctgcgggg taaaatggaa tcggtagtcg agttcgtggc
     3781 ctatgtgctg agccagctcg ccggggccat cgtcgctgcc ctcgcgactt acgtcatcgt
     3841 ggggcagacg ctcaaagttg ctccgggccc ctccgcgtcg actctcgggg cgctgttggt
     3901 cgagatcctt ttcaccttcg cgctcgcatt ggtagtcctc a
//
LOCUS       DASRJQ010000229         2527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3688575, whole genome shotgun sequence.
ACCESSION   DASRJQ010000229 DASRJQ010000000
VERSION     DASRJQ010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3688575"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(234..713)
                     /locus_tag="VFB89_05140"
     CDS             complement(234..713)
                     /locus_tag="VFB89_05140"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HZM26717.1"
                     /translation="MRVAVLTVSDAGARGERADASGDAIVAWVAGRGDKVVERTLVPD
                     EPEQIGRVLRRWADEDLADLALTTGGTGLTARDVTPEATRAVLEKEAPGIAEALRFVA
                     YPRFPRAALSRGLAGVRKRTLIINLPGSPGGVRDGLGILADIVEHAVALVRGERTEH"
     gene            complement(717..1826)
                     /gene="gcvT"
                     /locus_tag="VFB89_05145"
     CDS             complement(717..1826)
                     /gene="gcvT"
                     /locus_tag="VFB89_05145"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HZM26718.1"
                     /translation="MPELKQTPLADVHRALGAKMVAYAGFEMPVQYPTGILAEHQMVR
                     ANVGLFDISHMGEFEITGPDRNALLNRITCNDVSAIGAGGVQYSAILTPEGTFVDDCT
                     LYRFDNKWMVVVNAANTAAAWKHLVDHKGGANVRLKDISDEVGLLALQGPKSAALLQS
                     LTSVDLGELAYYHFVPGQVAGVDCFVSRTGYTGEDGFELYCRDRSVGVLWSALTSAGA
                     PPIGLGARDSLRLEMGYALYGHEIDRTITPLEAGLGWIVKLDKGSPFVGDRALRDQQS
                     RGVTRKLAGFRLLGRGIPRPGYPVWLDDRQVDVVRSGTMSPSLAMPIGTTFLPPESAK
                     AGTKFEVDCRGERLEAEVVSRPFWKNGTAGKRPRA"
     gene            complement(1905..>2527)
                     /locus_tag="VFB89_05150"
     CDS             complement(1905..>2527)
                     /locus_tag="VFB89_05150"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HZM26719.1"
                     /translation="DGVMIRTFDQAEVEELARFATVPVINGLTDLQHPCQILADLLTV
                     REAVGGWDGKVIAWNGDGNNMAHSWLDAAGLLGFELRLACPEGYQPNAEIVARNRALT
                     KIVLTTNPAEAARGAHVINTDVWASMGQEAEQEQRAAAFRGYLVDAALMRQADTRAIF
                     LHCLPAHRGEEVSDEVIEGSQSRVWDEAENRLHVQKALMATLMGTA"
BASE COUNT          451 a          868 c          778 g          430 t
ORIGIN      
        1 ccacctcggg gtcctcacgc cgcaccgagc cgcgagcgtc gtccaacgac gaaaacatcg
       61 cggtcgacaa gccggccgcc tcgagcgcgg cgttcacccg caccgcggtc tcgaggtcgt
      121 ccatggtgat gagaacttgg ctgggcataa ccccacaaga gcgtgaacgg tgaacggtaa
      181 acgggaggat gggaagggcc cgttcaccgt ttaccgatca ccgttcacgc agtttagtgc
      241 tcggttctct ccccccgcac caatgctact gcatgctcca cgatatcggc gaggatgccg
      301 agcccgtcgc gaactccacc cggactcccg gggagattga tgatcagggt acgctttcga
      361 acaccggcca ggccccgcga gagcgccgcg cgcggaaagc ggggatacgc aacgaagcga
      421 agcgcctccg caatccccgg cgcctccttc tccagcacgg ctcgggtggc ttcgggcgtc
      481 acgtcgcggg cagtgaggcc ggtgccgccg gtggtgagcg cgagatcagc caggtcctcg
      541 tcggcccacc gccggaggac gcgaccgatc tgctccggtt cgtccgggac gagggttcgc
      601 tcgaccacct tatcgccccg gcccgccacc caggcgacga tcgcgtcccc tgacgcgtca
      661 gctcgctccc ctcgcgcgcc agcgtcggaa accgtgagaa ccgcgacccg caccggtcag
      721 gccctgggcc gttttccggc cgttccgttc ttccagaaag ggcggctcac gacctcggcc
      781 tccaatcgct cgccccgaca atcgacctcg aacttcgttc ccgctttcgc tgattccggg
      841 ggcaggaagg tcgttcctat aggcatggcg agcgacggac tcatggtgcc gctccgaacg
      901 acatcgacct gtcgatcgtc gagccagacc ggataacccg gccgggggat accgcgcccc
      961 agcagccgaa agcccgccag cttccgggtg acgccccggc tctgctggtc ccgcagagcc
     1021 cggtcgccca cgaagggcga tcccttgtcg agcttcacga tccagccaag tcctgcctcg
     1081 agcggcgtaa tcgtgcgatc gatctcgtga ccgtagagag catagcccat ctccagccgg
     1141 agcgagtcgc gagcaccgag cccgatcggg ggcgcgcccg cggatgtgag cgccgaccat
     1201 aggaccccga cgctccgatc tcggcagtac agctcgaagc cgtcttcgcc ggtgtaaccg
     1261 gtgcgagaca cgaaacaatc gacgccggcc acctggccgg gcacaaagtg gtagtacgcg
     1321 agctcgccaa gatcgaccga cgtgagcgac tggaggagcg cggctgactt cggcccttgg
     1381 agcgccagca gcccaacctc gtccgagatg tccttgagcc gcacgttggc gccacccttg
     1441 tggtcgacca gatgcttcca ggcagccgca gtgttggccg cgttgacgac caccatccac
     1501 ttgttgtcga agcgatacag cgtgcagtcg tcgacgaacg tgccttccgg cgtgaggata
     1561 gccgagtact gcactccgcc ggcgccgatg gccgagacat cgttgcaggt gatccggttc
     1621 agcaacgcgt tgcgatccgg accagtgatc tcgaactcgc ccatgtgcga gatgtcgaac
     1681 agacccacgt tggcgcggac catctggtgc tccgccagaa tcccggtggg atactgcacc
     1741 ggcatctcga agcctgcgta agcgaccatc ttcgcgccga gcgcgcgatg aacgtcagcc
     1801 agtggggtct gtttgagctc gggcatggac acctgaactg cgtaaacggt gaacggtgaa
     1861 cggggctgat cggtgagcgg gacctcccgt tcaccgttca ccgtttacgc agttcccatc
     1921 agagttgcca tcaaagcctt ctgcacgtgc agccgattct cggcttcgtc ccagacgcgg
     1981 gactgcgacc cttcgatcac ctcatccgat acttcctcac ctcgatgggc ggggagacag
     2041 tgcaggaaga ttgcgcgggt atcggcctgg cgcatcagcg ctgcatccac gaggtagccg
     2101 cggaaggccg ccgcgcgctg ttcctgttcc gcttcctgac ccatcgacgc ccagacgtcg
     2161 gtgttgatga cgtgggctcc ccgagccgcc tcggccgggt tggtggtcag cacaattttg
     2221 gtcagggcgc gattccgcgc gacgatttcg gcgttgggct gatagccttc cggacatgca
     2281 agcctaagct cgaacccgag caggccggcg gcatcgagcc agctgtgcgc catgttgttc
     2341 ccgtcaccgt tccaggcaat gaccttccca tcccatccac ccaccgcttc ccgtaccgta
     2401 aggagatcgg ccaggatctg gcaggggtgc tgcagatccg tcaatccgtt gatgaccggt
     2461 accgtcgcaa accgggcaag ctcctcgacc tctgcctggt cgaacgtgcg aatcatgacg
     2521 ccgtcca
//
LOCUS       DASRJQ010000230         2401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_346065, whole genome shotgun sequence.
ACCESSION   DASRJQ010000230 DASRJQ010000000
VERSION     DASRJQ010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346065"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VFB89_05155"
     CDS             <1..512
                     /locus_tag="VFB89_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26720.1"
                     /translation="SAELLHALAELGVGLHIQAVNDDLLAFQSHITEAPSAILCMGDT
                     IMHLPERRAVESLIERAFAELMPGGKFVVSFRDYSAALSGDQRFVPVRSDDTRILTCF
                     LEYEPEVVLVHDILHERTPEGWQTRVSHYRKLRLSPAQLIASLEGRGFNVRREVGMKG
                     MVRLVAQRI"
     gene            597..1691
                     /locus_tag="VFB89_05160"
     CDS             597..1691
                     /locus_tag="VFB89_05160"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HZM26721.1"
                     /translation="MTGQDLDRLRERIAQLDLELLTRVAERVQLARQLGELKHRQDLA
                     TVDFPQERVVLERAGAVAKERGLDPRVAEDLIAGLIRASVTAQDDDRLRLAAVGAGKS
                     AVIVGGAGRMGRWLGRFLSAQGYTTGALDPALAPEENDRARRSLLSAELVVCSTPPAA
                     TAELYAKWSAKPPSGVVVDIASIKTPLMEPIRALQGAGGRVASIHPMFGPSAVLLRDA
                     DVVICDTGDPEATSTVERLFEPTTVHIVHMPLSDHDRIMADLLSLAHATAIAFALALP
                     RTDHPVRSTTFQALESLAATVVRESPDVYYEIQAMNPHSGVALERLRTALDRIVAAVR
                     ARDSQAFRALLEEGRGRTPEGNMAVRRFEK"
     gene            1714..1932
                     /locus_tag="VFB89_05165"
     CDS             1714..1932
                     /locus_tag="VFB89_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26722.1"
                     /translation="MKWNDWVRQMHRWVSIAFTVAVIATFVALAQKEPIVWVSYVPLL
                     PLALLLFSGLYLFVLPYVTKWRSGRRTD"
     gene            1953..2366
                     /locus_tag="VFB89_05170"
     CDS             1953..2366
                     /locus_tag="VFB89_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26723.1"
                     /translation="MAYAAALWAAVFAVFHIVWAAGWYPLLDAQGARIAFATPWKWAF
                     DVVVAAMCVVAVPVALAPVTTWGRRLPQTLIGTLAWVGSALLVLRLAAAMVQTGYLVA
                     VGRFRIAMIGIWDWWFLLGAILFSLSTWRSARMRC"
BASE COUNT          384 a          742 c          819 g          456 t
ORIGIN      
        1 catcggccga gctactgcat gcgcttgcag agctgggcgt tggcctgcac atccaggcgg
       61 tcaacgatga cttgctggcg tttcaaagcc acatcaccga ggcgccgtcc gcaatcctgt
      121 gcatgggcga cacgattatg catctgccgg agcgtcgtgc cgtcgagagc ctcatcgaga
      181 gagccttcgc cgagctgatg cccggcggca agttcgtggt ttcattccga gactactccg
      241 cggcgctcag cggggaccaa cgcttcgttc ccgtgaggag cgacgacacg cgcatcctga
      301 cttgctttct ggagtacgag ccggaggttg tcctcgtcca cgacattctt catgagcgca
      361 cgccggaagg atggcaaacc cgggtcagtc actaccgaaa gctacggttg tcacccgcgc
      421 aattgatcgc tagccttgag ggtcgagggt tcaacgtccg tcgcgaggtc ggaatgaagg
      481 gcatggttcg acttgtggca caacgcatct aacgcattgt tccagccgac gtgctacggc
      541 ggctccgcaa gcggctgaac tcaagcgtga gaaggctcta ggagcggcgg tcttccatga
      601 cggggcagga tttggacagg ctccgggagc gcattgcgca gctcgatctg gagctgctga
      661 cgcgggtggc ggagcgtgtg cagctggccc ggcaactggg cgagctcaag caccgacaag
      721 acctcgcaac cgtggacttc cctcaggaac gggtcgtgtt ggagcgcgcg ggcgccgtcg
      781 cgaaagaacg cggactggat ccgcgggttg ccgaagatct catcgccggg ctcatccgcg
      841 cctcggtgac ggctcaggac gacgacagac tgcgcctggc ggccgtgggc gccggcaaaa
      901 gcgcggtcat cgtcggtggt gctggtcgga tgggccgatg gctgggccgg ttcctatcgg
      961 cacaggggta caccacgggt gcactggacc cggccctggc gcccgaggag aacgacaggg
     1021 ctcgccggtc gctgctgtcg gcggaactgg tcgtgtgctc cacgccgccg gccgccaccg
     1081 cggagctgta cgcgaagtgg tccgcaaagc caccgtcggg agtggtcgtc gatatcgcca
     1141 gcatcaagac accgctcatg gaaccgatcc gcgctcttca gggggcaggc ggtcgcgtcg
     1201 cctcgatcca cccgatgttc gggccgtccg ccgtcttgtt gcgcgatgcc gacgtggtga
     1261 tctgcgacac cggcgacccg gaagccacct cgacggtcga gaggcttttc gagccgacca
     1321 ctgtccatat cgtgcacatg ccgctttccg accacgaccg catcatggcc gacctcctga
     1381 gtctggcgca cgcaaccgcg atcgcctttg cgctcgcgtt gccaaggacc gaccacccgg
     1441 tgcgcagcac gaccttccag gcattggagt cgctcgccgc aaccgtcgtg cgtgagagcc
     1501 cggacgtcta ctacgagatt caggccatga acccacattc gggggtcgcg ctcgagcggc
     1561 tgaggaccgc cctggatcga atcgtcgccg cggtcagggc gcgggactcg caggcctttc
     1621 gtgctctgct tgaagaggga cggggtcgca ctccggaagg caacatggca gtgcgtcgtt
     1681 tcgagaaatg atgcgactgg agaggaggcc cccttgaagt ggaacgactg ggttcggcag
     1741 atgcaccgct gggtgtctat tgcctttacg gtggccgtca tcgccacctt cgtcgccctg
     1801 gcgcagaagg agccgatcgt ctgggtgagc tacgtgccgc tgctcccgct cgccttgctg
     1861 ctgttcagcg gtctgtactt gttcgtgttg ccgtatgtca ccaagtggcg cagcgggcga
     1921 cggaccgact gaacgggcgg ctgggggttt gggtggccta cgctgcggca ctctgggcgg
     1981 cggtgtttgc tgtcttccac atcgtctggg cagcgggctg gtatccgctg ttggacgctc
     2041 aaggggcccg gatcgccttc gccaccccgt ggaaatgggc cttcgatgtg gtcgtggcgg
     2101 ccatgtgtgt cgtcgcggta ccggtggctc tggctcccgt gacgacatgg ggtcgacgac
     2161 tgccccaaac cctcatcggc acgctcgcct gggtcggttc ggcgctcctg gtgctccggc
     2221 tggcagcggc gatggtccag acgggctatc tggtcgcggt cggccggttt cgcatcgcca
     2281 tgattgggat ctgggattgg tggttcctcc tgggagcaat cctgttcagt ttgagcacgt
     2341 ggcgatcggc gcgcatgcga tgctgacgct gcgcgctgag tgccacatga ggatcgcgca
     2401 t
//
LOCUS       DASRJQ010000231         4951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_624799, whole genome shotgun sequence.
ACCESSION   DASRJQ010000231 DASRJQ010000000
VERSION     DASRJQ010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624799"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VFB89_05175"
     CDS             complement(<1..184)
                     /locus_tag="VFB89_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26724.1"
                     /translation="MADTVRETPRARTLVLDVPGWPGHRAGQHLDVRLTAEDGYQAQR
                     SYSIASAPEDARVVLTV"
     gene            complement(268..891)
                     /locus_tag="VFB89_05180"
     CDS             complement(268..891)
                     /locus_tag="VFB89_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HZM26725.1"
                     /translation="MTSPFSRGFRGRPRSAAAAGRLPPGQYETRDFPVLSAGPTPRIS
                     LATWDFTLLGPGGQTARWTWDEFQALHREPVTADIHCVTKWSKLGTVWEGVSVDTLLA
                     DAAPRDVRPAPYVLAVCDGDYTTNLPLADVTGGKAWVALSYGGEPLAPEHGGPARLLV
                     PHLYFWKSAKWLRQLQFMERDQPGFWESHGYHNYGDPWREQRYAGDE"
     gene            complement(888..1013)
                     /locus_tag="VFB89_05185"
     CDS             complement(888..1013)
                     /locus_tag="VFB89_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26726.1"
                     /translation="MSASTLRDTKHAEVATTHGQVTRTLTGRSCAVLTNGQAEGG"
     gene            complement(1558..3780)
                     /locus_tag="VFB89_05190"
     CDS             complement(1558..3780)
                     /locus_tag="VFB89_05190"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM26727.1"
                     /translation="MADLRTLLEPDFAGAYTIERELGRGGMATVFLARDLKHHRPVAL
                     KALHPHLSHVLGPERFRREIEFLAGLTHPHIVPLHDSGGSAGLLWYTMPYVDGESLRD
                     RLRREGRLPLEEALRITTEVADALHYAHGRGIIHRDLKPENILLSAGHAFVADFGIAR
                     ALMHPGDDRLTGTGFALGTPAYMSPEQAAGEHAVDARSDIFALGCVVYEMLAGEPPFT
                     GPTPQAVLAQRLTGPAPPLRSRRADLSQSVAAAVARALALDPVDRFSTATGFATSLAA
                     AMDQTGATTAATAPAAPGATNDRSIAVLPFSDMSAERDQEYLADGIAEEIINALTRVG
                     SLRVAARTSSFAFKGRNEDVGQIGRKLKVTTILGGSVRKAGNRLRVTAQLVNATDGYQ
                     LWSERYDRELADVFAVEDEIAESVVSALSIVMGETGGRGRGIDRPRTDNVHAYEYYLR
                     GRQLFHQFRKKQIQLARRMFERAIELDPLFTLAYAGAADCSSILYMYFEATEANLQLG
                     DALSLRALELGPDIAEAHAARGLVLTLRGSFEAAQREFESAIALDPRLYETWYFYARV
                     CFEKGQLAEAARFFEQAAALRPDDYQAVSYLSMTNEAMGRKVEANSASRRALPILEQH
                     LELNPDDARAWYSGATELSRLGEREKAFQWVNRALALDPEDGAVLYGIACVYALLGED
                     ERALDALERGIRAGFGRREWAEKDPDFASLRESPRFRKLLETMPTHFGSVHPTAGSAE
                     "
     gene            complement(4224..>4951)
                     /locus_tag="VFB89_05195"
     CDS             complement(4224..>4951)
                     /locus_tag="VFB89_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26728.1"
                     /translation="PVRIARAWIVLMKRLGYKRFVAQGGDWGGVVVDMMAVEGAPELI
                     GIHTNMPGAVPADVWKAIVSGGPAPSGLSDEERQRYEKLKAWFAKEVAYALQMGLRPQ
                     TLYGIADSPVGLAAWMIDHDLLSYQAISRLFVDGTPYGAMTRDDVLDNITLSWLTTTG
                     ISAGRLYWENKLPFFEPKGVSIPVAVSVFPDEIDAAPRSWAERAYPKLIHYNQLDQGG
                     HFAAWEQPKLLVEELRTGLKSLR"
BASE COUNT          863 a         1587 c         1624 g          877 t
ORIGIN      
        1 cgaccgtcag cacgacgcgc gcgtcctccg gcgccgaggc gatggaataa ctccgctgcg
       61 cctggtagcc gtcctccgcc gtgaggcgca cgtccaggtg ctgccccgcg cggtggccag
      121 gccaacccgg gacatcgagc acgagcgtcc gtgcccgcgg cgtctcccgt acggtgtcgg
      181 ccacggtggc gagtcgccac gcgatcgacc gcgccgtcgg ccgcagcgtg gaagccgccg
      241 cggggctgga gggcggtggc gcgcggttca ctcgtcgccg gcgtaccgct gctcgcgcca
      301 cgggtcgccg tagttgtgat agccgtgcga ctcccagaac ccgggctggt cgcgctccat
      361 gaactgcagc tgccgtagcc acttcgcgct cttccagaag tagaggtgcg gcaccaggag
      421 tcgcgcgggg ccgccgtgtt ccggcgcgag aggctcgccg ccgtagctca gcgccaccca
      481 cgccttgcca ccggtcacgt cggcgagcgg cagattcgtc gtgtagtcgc cgtcgcacac
      541 cgcgagcacg tagggcgcag gccggacgtc ccgcggggca gcatccgcga gaagcgtgtc
      601 cactgagacg ccctcccaga ccgttccgag cttggaccac ttggtgacgc aatggatgtc
      661 cgcggtgacg ggctcgcgat gcagcgcctg gaactcgtcc cacgtccatc gcgcggtctg
      721 cccgcccgga ccgaggagcg tgaagtccca cgtcgcgagc gagatgcgcg gggtcggacc
      781 cgccgaaagt acaggaaaat cccgcgtctc gtactggccg ggcgggagcc ggccggcggc
      841 ggccgcagac cgtgggcggc cacgaaaccc gcgggagaat ggcgacgtca tcctccctcc
      901 gcctggccat tcgtcagcac cgcgcaggaa cggccggtga gggtccgcgt cacctgccca
      961 tgcgtcgtcg cgacctcggc gtgcttcgta tcgcgcagag tggatgcgct catggccagc
     1021 tctcattctt ctcgaaaggt catcgccgct gctggtgaac ccctgacggc gaaaaacaac
     1081 accgcccgag cggaagcaag tctgcaggac ggtaccgctt tacgccagcg ggccagacgg
     1141 actgctaccg tttctggtac agtttctccg ggcaaacccg gtcatttcgg gcagggaagc
     1201 gggcaccgat gctcggtggg tgaccgcttc caggcaactt ggccggccgg caccgactct
     1261 ggatcaggag agtcctggtt cgagcccagg agggggcaat gcccggggag aaatgtccgg
     1321 gcctttgctt tagggctcgg cctgcggctc gaccccgggg gggacaactc gaagcgcgat
     1381 gggggaatat ctgctgtcgc gcttcgactc cttagggcac tcttcagtga gtatgtgggg
     1441 tgtaggtccg tatgtcactt cgcagccaat tgtgccactg ctcgtgctcc gaatggtgga
     1501 gtgagaagcg agcacacgcg atttctgcca tcgatttctg ccatcaatgt ccatcactca
     1561 ttcagcggat cccgccgtcg gatggaccga gccaaagtga gtcggcatgg tctccaggag
     1621 cttccgaaac cttggcgact ctcgaagcga ggcgaagtcc ggatctttct ctgcccactc
     1681 ccgccgaccg aagccagctc ggatcccccg ctcgagggcg tcgagcgccc gctcgtcctc
     1741 gcccagcaaa gcgtacacgc aggcgatgcc atacagcaca gcgccgtcct cagggtcgag
     1801 ggcgagcgca cggttcaccc actggaaggc cttctcccgc tcacccagtc ggctgagttc
     1861 ggtggctcca ctataccatg cacgagcatc atcggggttg agttcgaggt gttgctccaa
     1921 gatcgggagc gcgcgtcgag aggccgaatt ggcctcgacc ttacgcccca tcgcctcgtt
     1981 cgtcatcgag agataggaga cggcctggta atcgtcgggt cgaagcgccg cagcctgctc
     2041 gaagaaacgt gccgcctcag ccaactgacc cttctcgaag cacactcgag cgtaaaagta
     2101 ccaggtctca tagaggcgcg gatcgagcgc gatggcggac tcgaactccc gctgggcggc
     2161 ctcgaaactc ccccgcagtg tgagcacgag cccgcgcgcc gcatgggcct ccgcgatgtc
     2221 cgggccgagc tcgagcgccc tcaggctcag cgcgtccccc agttgcagat tggcctcagt
     2281 cgcctcgaag tacatgtaca agatcgagct gcagtccgcc gcccccgcgt aggccagggt
     2341 gaacagggga tcgagctcga tcgcgcgctc gaacatgcgg cgtgcgagct ggatctgctt
     2401 cttgcggaac tgatggaaga gctgccggcc ccggaggtaa tactcgtacg cgtggacgtt
     2461 gtcggtacgc ggccggtcga ttcccctgcc gcgtccgccg gtctctccca tgacgatcga
     2521 cagcgcgctc acgacgctct ctgcaatctc atcctctacc gcgaacacgt ccgccagctc
     2581 tcggtcgtag cgctcagacc agagctggta accatcggtg gcgttgacga gctgcgctgt
     2641 caccctcagg cgattgcccg ccttgcgcac gctgccaccg agaatcgtgg tgaccttgag
     2701 tttccggccg atctggccaa cgtcctcgtt tctcccttta aaggcgaacg aggaggtacg
     2761 cgcggcgaca cggagcgagc cgactctggt cagcgcatta atgatctcct ccgctatgcc
     2821 atcggccaaa tactcctgat cgcgttcggc gctcatgtcg ctgaatggaa gcacggcgat
     2881 ggagcgatcg ttcgtagcgc caggcgcggc tggagcggtg gccgctgtgg ttgccccggt
     2941 ctggtccatc gccgcggcca agctggttgc gaacccggtg gcggtggaga atctgtccac
     3001 cggatccagc gcgagcgcgc gcgcgactgc cgccgctacc gattgggaga ggtccgcccg
     3061 gcgcgagcgc aatggggggg ctggcccggt aaggcgctgc gcgagcacgg cctgtggggt
     3121 cggtcctgtg aacggcggct cacccgcgag catctcgtac acgacgcagc cgagggcaaa
     3181 gatgtcgctc cgggcgtcga cggcgtgttc gcctgcagcc tgttcgggac tcatataggc
     3241 cggtgtcccg agagcgaacc cggttccagt gaggcggtcg tctccggggt gcatcaacgc
     3301 ccgagcaatg ccgaagtccg cgacgaatgc atgcccggcg gaaagcagga tattctcggg
     3361 tttgaggtcg cggtggatga tgccgcggcc gtgagcgtaa tgcagtgcat cggcgacctc
     3421 cgttgtgatc cgaagcgcct cctcgagcgg caatcgccct tcccgccgaa gccggtcccg
     3481 aagcgactcg ccgtccacat acggcatcgt gtaccacagg aggcccgcac tgccgccgga
     3541 atcgtgaagt gggacgatgt gcggatgagt gagaccagca aggaactcga tctcacgtcg
     3601 gaaccgctca ggtcccagga cgtgggagag gtgggggtga agagccttca gggcaacggg
     3661 tcggtggtgc ttgaggtctc gggccaggaa taccgtagcc atgccgccgc gcccgagttc
     3721 gcgctcaatg gtataggcgc ccgcgaagtc tggttccagc agagttcgga ggtctgccac
     3781 tgtgggcctg gctctgggga gggagttagc gactcttgga ggatacacgg cgggggggtg
     3841 gagagcgaga tcaggtcacg cattcggacg ctggagtggt ccgagggaca ggccaactcc
     3901 gacattcgag tgagtaaagt acggcaaaga tttaggggcc ctgcttgccc ggcgagttga
     3961 atgtcacttt tgccctgtga aaacgttccc ttcgggtcca ccacctcgat cagcaccttg
     4021 tgctggctac gtggcatgcc gaccaggatg acggtgttgc tctgaccgta atccgcccac
     4081 tgccaaggca ggtcgttgag cgggtcattt gcatttctct cctttgaaga ttcgcctcgg
     4141 cgctcgtcgg cgccgcgggg gtatcattct aggcggcgga tgacgagcac gtccggcccg
     4201 ccatccaccg cccgaccttt gagctaccgc aacgacttga gccctgtgcg cagctcttcc
     4261 accaagagct tcggctgttc ccaggctgca aagtgcccac cttgatcgag ctggttgtag
     4321 tggatgagtt tgggatacgc ccgttcggcc cagctgcgcg gggccgcatc gatctcgtca
     4381 ggaaacacgc tcacggcgac cgggatggag acgcctttcg gttcgaagaa gggcaacttg
     4441 ttttcccaat agagacgtcc cgcggagatg cccgtggtcg tcaaccagga gagcgtgatg
     4501 ttgtcgagga catcgtctcg ggtcatagcg ccatagggcg tgccatcgac gaagaggcgc
     4561 gagatagctt ggtaactcag gaggtcgtgg tcgatcatcc aggcagccag gccgacgggt
     4621 gaatccgcaa ttccgtagag cgtctgtgga cggagtccca tctgaagggc gtaggccact
     4681 tctttcgcaa accaggcttt cagcttctcg taccgctgtc tttcctcgtc ggatagaccg
     4741 gatggcgccg ggccgccgga cacgatggcc ttccacacgt cggctggaac agcgcctggc
     4801 atattggtgt gaatgccgat caattcggga gcgccttcta ccgccatcat atcaaccaca
     4861 accccacccc aatcgccgcc ttgggcgaca aagcgcttgt agccgaggcg cttcatcagc
     4921 acgatccagg cacgtgcgat acgcacgggg t
//
LOCUS       DASRJQ010000232         2963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_685522, whole genome shotgun sequence.
ACCESSION   DASRJQ010000232 DASRJQ010000000
VERSION     DASRJQ010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685522"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(393..893)
                     /locus_tag="VFB89_05200"
     CDS             complement(393..893)
                     /locus_tag="VFB89_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26729.1"
                     /translation="MFDHLVSERPHPSLGTHAETYGRFIGSWRGTYRDPHPEGEESGP
                     LEVHFAWALEGRAVQDVWIAPAPPLEADRTYRRQMYGTTLRVFDPATQLWRVDWWNPG
                     RGVHCALVGRRLGEQIVQLGYWDDRPQRWRFLDITPNAFRWQAHRLDDNGESWSLVTE
                     FALQRI"
     gene            complement(1102..2322)
                     /locus_tag="VFB89_05205"
     CDS             complement(1102..2322)
                     /locus_tag="VFB89_05205"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HZM26730.1"
                     /translation="MQDSMTIRRIAWTAATLACWWAPTVGAQTPERSPDAASLRRISS
                     FSWTQAQLQFGFAHWDEVYPARIIQRGPSMRPLPAGKPIAALAEGMPGAQELEQFIAK
                     QKVTGLIVLQDGAVRLERYALGYSDSGRWVSQSVAKSLTSTLVGAALKDGFIKSLDDA
                     ITQYIVDLRGSAYDSVLVRQLLTMTSGVRWTEAYTDSTSDIARFYREPVTPGLDATVS
                     YMRRLSREAPPGTKWVYKTGETHLLGVLVAAATGQSLADYLSATIWKPYGMERAASWS
                     TDRSQHELAGCCLQASLRDFARFGQFVIDGGRIGGRSVVPEGWFEAATKTQVATSYPG
                     FGYGYQWWTREDGTFDAIGIHGQLIHIDPARRLVVAINSAWPEATSPDRSAARNRMIA
                     TLAAMLDAEAKVKP"
     gene            complement(2496..>2963)
                     /locus_tag="VFB89_05210"
     CDS             complement(2496..>2963)
                     /locus_tag="VFB89_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26731.1"
                     /translation="ARSLRLLDREEEWVGDLRHAIELQPRLPVSWLDLAIYHARRGAP
                     DSVRTASARFLEYRSESGRAPAGASDALTRALMGTASVAAFLRTVPSDSATDWSWLAV
                     QLYAMRGESDSAFSKLRKLESSRGIDFPTTAPFLAGYLGGDARWRTVRKRLGL"
BASE COUNT          540 a          950 c          940 g          533 t
ORIGIN      
        1 gcaatgatgc tctgctggta tcccacaagg tgccacagct cgtcgtagat ggagtgtgac
       61 gcgccctccg gccggagcgt cacctgctcg agcgtcaacc ctgacagcag gtgcgctctc
      121 tccgcgaact caccggcgag aaacagatgt tcgaggatat gcacgcctac cctgactctt
      181 ctttgcttgt tatgaatgat tgatctacgc cgcctaacgc cccgcataag cggcagggcc
      241 gcggcgagat cgccagatct tgccggagaa cctgcaggtg gtgatggctt tgcgcaaccg
      301 ttggccgcgc cctgtccgct tcatgcggtt gttagatggc agcctttgca cacgcgactt
      361 gccatgggtg gcttgcagtc cggaggtcgc tattaaatac gctggagggc gaactcggtc
      421 actaggctcc acgactcccc gttgtcatca aggcgatgcg cctgccagcg aaaggcattt
      481 ggggtgatat cgaggaaccg ccaccgttgg ggccgatcgt cccagtaccc gagttggacg
      541 atctgttctc ctaagcggcg tccgaccagt gcacagtgta ctccgcggcc gggattccac
      601 caatccacgc gccagagctg cgtggccggg tcaaacaccc ggagggtggt tccgtacatc
      661 tgcctccggt acgtccgatc agcctccaag ggcggggcgg gtgcgatcca gacgtcttgg
      721 acagcgcgac cttcgagggc ccaagcgaag tgtacctcga gcgggccgga ttcctcgcct
      781 tcggggtggg ggtctcgata cgtcccccgc caactgccga tgaaccggcc gtacgtctcg
      841 gcgtgggttc ccaggctggg atgggggcgt tccgagacaa ggtgatcaaa catggggata
      901 cgtccgtcgc ggtgatggct gaagaatagc aagggtgaac aggcgagggc cggggctagg
      961 gagggaggcc gggccaagcg cccagaccgc tgccatctaa cgacacggaa atgagccgcg
     1021 gggcgctgcc ttcttggatc tcaggagcac acaggaagct cgaggcggcg gaccggcggc
     1081 tccatttcct ggttagacgc cttacggctt gacctttgcc tccgcgtcga gcatcgccgc
     1141 gagggtcgct atcatgcgat tccgtgcagc ggagcggtct ggactcgtcg cttccggcca
     1201 ggcgctattg attgccacca caagccgccg cgccgggtcg atatgaatca gctgcccatg
     1261 gatgccgatc gcatcaaaag ttccgtcctc tctggtccac cactgatacc cgtagccgaa
     1321 ccccgggtac gacgtcgcta cctgggtctt cgtggcagcc tcaaaccacc cttcaggaac
     1381 aacgctacga ccgccgatcc gcccaccgtc gatcacgaac tggccaaagc gagcgaagtc
     1441 tcgcaatgat gcctggaggc agcagcccgc cagctcgtgc tggctgcgat cggttgacca
     1501 ggaggccgcg cgctccatcc cgtacggctt ccagatggtt gcgctgaggt aatcggcgag
     1561 ggactgccct gtggcggcgg cgacgaggac gccgagcaga tgggtctcac ctgtcttgta
     1621 cacccatttg gtgccgggcg gtgcctcacg cgacaagcgc cgcatgtaac tcaccgtcgc
     1681 atcgagtccg ggcgtcaccg gctcccgata gaaccgtgcg atgtccgagg tcgaatcggt
     1741 gtaggcctcg gtccagcgga ccccggaagt catcgtcaac agctgccgga ccaagacgct
     1801 gtcgtaggcg ctgccgcgca ggtctacgat gtattgggtt atcgcgtcgt cgaggctctt
     1861 gatgaaccca tccttcaggg cagcgccgac cagcgtgctg gtcagcgatt tcgcgaccga
     1921 ctgcgagacc cagcgacccg aatcgctgta tccgagggcg tagcgctcga gccgcaccgc
     1981 cccgtcctgg agcacgatta acccggtgac cttttgcttg gcgatgaact gttccagttc
     2041 ttgtgcgcca ggcattccct ccgcgagtgc ggcgatcggc tttccagcgg gaagtgggcg
     2101 catgctgggc ccgcgctgga tgatccgggc agggtagact tcgtcccaat gggcgaagcc
     2161 gaactgcagc tgagcctggg tccagctgaa cgaggaaatg cgacggagcg aagcggcgtc
     2221 cggtgaacgc tcgggagtct gggcgccaac cgtgggcgcc caccagcagg cgagcgttgc
     2281 cgcagtccac gcgatgcgcc gaatcgtcat gctgtcctgc aatcaggtgt cacggctcgc
     2341 tccgccaagc atcctccatg acaggcgtct aacgtcctgc gggtacagac cgatgacatg
     2401 ggggacatgg ctcacgttga cccagtggtg gtcccagcgg attccgccgt tggtactcag
     2461 aagctggtct cgaagtgacg cggtactccg ggccgctaca ggcccagccg ctttcgtacc
     2521 gtgcgccagc gggcgtcgcc acccaaatag cccgcaagga acggcgcagt ggtcgggaaa
     2581 tcgatgcctc tgctgctctc gagcttgcgc agcttgctga aggcactgtc tgactctcca
     2641 cgcatcgcgt acagctgcac ggcaagccac gaccagtcag tcgcgctgtc gctcggtaca
     2701 gtccgtagaa aagcggcgac cgatgcagtt cccatcaacg cgcgggtgag ggcatctgat
     2761 gcccccgccg gcgcgcggcc cgattcgctc cgatactcga gaaaccgcgc actcgccgtt
     2821 cgcacgctgt cgggtgcgcc acgccgcgcg tgatagatcg caagatcgag ccaggacacc
     2881 gggagcctag gttgcaactc aatggcatgc cgcagatccc ccacccactc ctcttcacga
     2941 tcgagcaggc gaaggctgcg cgc
//
LOCUS       DASRJQ010000233         5374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1470994, whole genome shotgun sequence.
ACCESSION   DASRJQ010000233 DASRJQ010000000
VERSION     DASRJQ010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470994"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VFB89_05215"
     CDS             <1..433
                     /locus_tag="VFB89_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26732.1"
                     /translation="AGHHADVVIETSWDDLGRVITKGASLPLSGEPREISGHARDRYL
                     YAPIDGVFRTQAGIGDLVHQGQDIAGIHHAGGMLTLAAPLDGVLRGLTRDGVPVTAGT
                     KVLEIDPRGRVGEVTGINERPRRIADGVLAAVRSRQEIASK"
     gene            469..1104
                     /locus_tag="VFB89_05220"
     CDS             469..1104
                     /locus_tag="VFB89_05220"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HZM26733.1"
                     /translation="MSGVKDCCEIRADVPERQRRVLHVVLWVNAGMFLAEFGAGILAH
                     STALLADSVDMLGDAIVYGFSLYAVARGGVWQARSALLKGVIMAAFGIGVLVEVASKI
                     IRGVVPTPDVMGGVGTIALAANVFCLWLLWCRRSDDINMASAWVCSRNDVVANIAVLI
                     AAGAVALMGSPWPDIVVGLLIAGLFGSSAVRVIRGARRELHVAGASAWRTP"
     gene            1101..1385
                     /locus_tag="VFB89_05225"
     CDS             1101..1385
                     /locus_tag="VFB89_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26734.1"
                     /translation="MKRTIALLGLGLVLVAGIAVAQQPPVQPGPTPGHQMPMQGGMCP
                     MIGGMMGGGMMGMMGGQSDSPRMMQMRGEMMKAMGEVLVKYGKAMEGTTR"
     gene            1725..3437
                     /locus_tag="VFB89_05230"
     CDS             1725..3437
                     /locus_tag="VFB89_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZM26735.1"
                     /translation="MGLERFRKGAALRCVPLLTVLLLIIAPAVGGSQSPGKPKVDRLV
                     MGLITPYLDYVRPWINGTADHNIQHDPMQEWLIEVDPSGQYKPWLADSWTIGPNGRSW
                     RVKLHKGVQFHHGYGEFTAQDVIHTHALWCDPNYPGRKDAPVAGYKAGICQVEKIEMV
                     NPHEITMHCKVVCLDMPFYYSSASNVMIFSKKQWDAEGEMGYEKRPAGTGPYMFKERQ
                     LGRYVLYERAPTAHWKHGVVDWKEIKMTWVREEATRFAQIIAGETHLTEVNRDLTDEL
                     VAKGYKLIKSRDTAQQMQVNFGGLYFGTEDKATGRYTEYGGTAGKLDPKVPWTNIKVR
                     QAMNKAINRPELLRVIYKGRATPMYVHGYYPGLEGWDPTWEKRFPAMYGYDPAAAKRL
                     LAEAGYPNGFKAKAWLYPFAGAPEMIAVMEAVAVQLREVGIDIALEDADWVAAVRPKL
                     QKREAHGYLWAIPPSKKAVEPQLAVFNVGKGIPHIFQTDEIYKMWEELLQISDAKARD
                     AQLRKIGNYKFESFETIPLFDVNIEVIVDPKIVADWPFSGWDGGDIGHTWLIKACKQE
                     KPCK"
     gene            complement(3475..4284)
                     /locus_tag="VFB89_05235"
     CDS             complement(3475..4284)
                     /locus_tag="VFB89_05235"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZM26736.1"
                     /translation="MKCGAFTLALTLALGFWLVVIGTPYAQQAPDARVADLVRAGKLR
                     VGIGLAPVIATKDPATSELRGTGIDLGRALAARIGVEFEPVEYPSPPKILEGLDTGAW
                     DVAFLGVDPTRLAQVDFSPPYLQVDYTYLVPAGSSVREVGDADQPGIRIAVTRNSVGD
                     VALTRILKRAELQRVDTAAAGLEALRGGTVHVLASDRPNLLHLSAGLPGSRVLEDRYH
                     SFFLAMVVPKGQLGRHAYVSDFIEEAKTSGLVKQTVERAGLRGVQIAPAGR"
     gene            4513..>5374
                     /locus_tag="VFB89_05240"
     CDS             4513..>5374
                     /locus_tag="VFB89_05240"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HZM26737.1"
                     /translation="MKGLAVAAFDLDVLPCPRDNDGLMPISVLIDCDPGYDDVIAIML
                     ALACPEVEVLGITTVEGNQPVAETTENALRVVEFLARKIPVAEGADRPLVRELPFRRT
                     PSTDSADLRRDIPGLPPATGRAVDEHAVDLLARVLLAADEPVTLVPLGPLTNVALLLA
                     RYPDAARRIGRMVLMGGAIGEGNVTTAAEFNIYVDPESAQRVFSSGIDLTMVGLDVTY
                     RAIVSPAELEELRGLGRAGSVVAAILDAFPPYRRYSWYPEGYPIHDAVAVAELVQPGL
                     LTTEYVNVVVE"
BASE COUNT          953 a         1668 c         1827 g          926 t
ORIGIN      
        1 agcagggcat catgcggacg tggtgatcga gacgagttgg gatgatctcg ggcgggtcat
       61 caccaagggc gcgtcattgc cgctctcggg tgaaccacgc gagatttcgg gccatgctcg
      121 cgatcgctat ctctatgcgc ctatcgacgg cgtatttcga acgcaggctg gtattggaga
      181 cttagttcat caggggcagg acatcgcggg aatccaccat gctggcggca tgctgacgtt
      241 ggccgcgccg ctcgatggtg tgctccgcgg cctcacgcgc gatggcgtgc ccgtgacagc
      301 tggcacgaag gttctcgaga tcgacccacg tgggagggtc ggcgaggtta ccgggatcaa
      361 cgagcgccca cgacggatcg cggacggtgt cctagcggcc gtccggagcc ggcaagagat
      421 agcgtccaaa taggtgaccg cgcggcgtcc ttcacgccct gcgcgataat gagcggcgtg
      481 aaggactgct gtgagatcag ggcggacgtt cccgagcgcc agcggcgagt gttgcacgtg
      541 gtcctctggg tcaacgccgg gatgttcctt gccgagttcg gcgcgggcat cctggcccac
      601 tccacggcct tgctcgccga ttcggtggac atgctcgggg atgccatcgt gtacgggttc
      661 agcctgtacg cggtcgctcg agggggcgtc tggcaagcgc gatcggcgct cctgaaaggc
      721 gtcatcatgg cggcgttcgg catcggcgtc ctggtcgagg tcgcctcgaa gatcatccga
      781 ggcgtcgtcc cgacgcccga cgtgatgggc ggggtaggta ctattgccct ggccgcgaac
      841 gtgttctgcc tctggctgct ctggtgccgc aggagcgacg acatcaacat ggcctcggcg
      901 tgggtctgct ccaggaatga cgtagtggcg aacatcgccg ttctgatcgc tgctggggcc
      961 gtagccctca tgggctcccc gtggcccgac atcgtggtgg gcttgctcat cgctggcctg
     1021 ttcggcagtt cggcagtaag ggtcattcga ggcgcgagac gcgaactaca tgtggcgggg
     1081 gcctccgcct ggaggacgcc atgaagcgaa cgattgcgct cctgggactc ggtctcgtgc
     1141 tggttgctgg tatcgcggtc gctcaacagc ccccggtgca gcccggcccc acgccgggcc
     1201 accagatgcc catgcagggc ggcatgtgtc cgatgatagg cggcatgatg ggcggcggca
     1261 tgatgggcat gatgggcggg cagtccgact cgccccgcat gatgcagatg cgcggcgaga
     1321 tgatgaaggc gatgggcgag gtcctggtga agtacgggaa ggcgatggag gggaccaccc
     1381 ggtagggcgc cgctcgactc ggacggaact cgaacggagt cagtcgtgac gatgcgtccc
     1441 gagccgttcg acggctggga cgcggcgtgg tctaacgtgc ggattcctac gaatgaagca
     1501 ggatcacgcg gtgcgaagtg gtgggccgag ccggactgaa aatcctggtg gcggcggttc
     1561 aatcccgtcc ctgctcacca ttcttctcag gtagttaccg cagcaccggt agttgccgca
     1621 gcgtcgacca ctgccgagct gatctcgaac cggtgtgaac gccttcttga catccgacgg
     1681 tttctgtccc agcgtttgcg cactcagccg ccgaggaggg tgccatgggc ctcgaacggt
     1741 tccggaaggg tgccgcgctc cgctgcgtgc cgctgctgac cgtcctcctc ctgatcatcg
     1801 cccctgcggt cggggggtcg caatcgccgg gcaagcccaa ggtggaccgg ctcgtgatgg
     1861 ggctgatcac cccgtatctc gattacgtac gcccgtggat caacgggacg gccgatcaca
     1921 acatccagca cgaccccatg caggagtggc tcatcgaggt cgacccgtcg ggccagtaca
     1981 aaccctggtt ggcggatagc tggacgatag gccccaacgg gaggtcgtgg cgggtgaagc
     2041 tgcacaaggg cgtgcagttc caccacgggt acggcgagtt cacggcccag gacgtgattc
     2101 acacccatgc cctctggtgt gacccgaact accccgggcg caaggacgcg ccggtcgccg
     2161 gttacaaggc cggcatctgc caggtcgaga agatcgagat ggtcaatcca cacgagatca
     2221 ccatgcactg caaggtcgtg tgtctggaca tgccgttcta ttactcgagc gcgtcgaacg
     2281 tcatgatctt cagcaagaag cagtgggatg ccgaaggcga gatgggctac gagaagaggc
     2341 cggcggggac gggcccgtac atgttcaaag agcgccagct cgggcggtac gtcctgtacg
     2401 agcgggcgcc gacggcccac tggaagcacg gcgtggtgga ctggaaagag atcaagatga
     2461 cgtgggtccg cgaagaggcg acgcgcttcg cccagatcat cgccggcgag acccatctca
     2521 ccgaagtcaa ccgcgacctc acggacgagc tggtcgcgaa gggctacaag ctcatcaaga
     2581 gccgtgacac cgcacagcag atgcaggtga atttcggcgg gctctacttc ggcaccgagg
     2641 acaaggccac ggggcgctac acggagtacg gtggcaccgc cgggaagctg gaccccaagg
     2701 tcccctggac gaacatcaag gtgcggcagg ccatgaacaa ggcgatcaat cggccggagc
     2761 tgctccgggt gatctacaag ggccgggcga ctccgatgta cgtgcacggc tactaccccg
     2821 gcctggaagg ctgggacccg acctgggaga agcggttccc ggcgatgtat ggctacgacc
     2881 ccgccgcggc gaagcgcctg ctcgcggagg cggggtatcc caacggcttc aaggccaagg
     2941 cgtggctcta tcccttcgcc ggcgccccgg agatgatcgc ggtgatggaa gccgtggccg
     3001 ttcagctgcg cgaggtgggg atcgacatcg cgctcgaaga cgccgactgg gtggccgccg
     3061 ttcggccgaa gctgcagaag cgtgaagccc acgggtatct ctgggccatt ccgccctcga
     3121 agaaggccgt cgagccacag ctcgccgtct tcaatgtcgg caagggcatc ccacacatct
     3181 tccagacgga cgagatctac aagatgtggg aagagctcct gcagatcagc gatgcgaagg
     3241 cgagggatgc ccagctgcgg aagatcggta actacaagtt cgaaagcttc gagaccatcc
     3301 ccttgttcga cgtcaacatc gaggtcatcg tggacccgaa gatcgtcgcg gactggccgt
     3361 tttccggttg ggacggggga gacatcgggc acacgtggct gatcaaggcg tgcaagcagg
     3421 agaagccctg caaataggac gggcttccct gccggcagcg cggacacttc aagatcagcg
     3481 accggcaggt gcgatctgga ctcctcgcag gccggcgcgc tcgaccgtct gctttaccaa
     3541 tcccgaggtc tttgcctctt cgatgaagtc gctgacgtag gcatgccgtc cgagctggcc
     3601 cttcgggact accattgcca gaaagaacga gtggtagcgg tcctccagca cgcgcgagcc
     3661 gggcagtccg gccgaaaggt gaagcagatt tgggcggtcg cttgccaaca cgtggacagt
     3721 gccgccgcgt agcgcctcta gaccagcggc tgccgtgtcg acgcgctgca attcagctcg
     3781 cttaagaatg cgagtcaatg cgacgtcgcc gacgctattg cgggtgactg cgatgcgaat
     3841 tcccggctgg tcggcgtccc cgacctcgcg gaccgacgag ccagcgggca ccagatacgt
     3901 atagtccact tgcagataag gcggcgagaa gtcgacctga gcgagccggg ttggatcgac
     3961 cccgagaaac gccacatccc aggctccggt gtcgaggcct tcgagaatct tgggagggct
     4021 cggatattcg accggctcga actcgacccc gatacgtgct gccagcgcgc gacccagatc
     4081 gatcccagta cctctcaact cgctagttgc tgggtccttg gtggcaatga cgggagccaa
     4141 ccctatcccc actcgaagct tgccggcccg gacgaggtcc gcgacccgcg cgtccggcgc
     4201 ttgctgagcg tacggggttc cgataaccac gagccagaat cccaacgcca gcgtcagtgc
     4261 cagcgtgaaa gctccgcatt tcacgggtag cctcccttcg agcccttgcg gcgtcggcga
     4321 tgagcccccg gcgtctgtgc ccagagtgtg cccaactcgg gctcactcgg gatgattctg
     4381 gaacactctg gctacgataa ccgactgggc ctggtgttcg acggcgagac tgtatgcgca
     4441 cctgcatgtg ctgcgttcca gatctgagaa tcctggtgtc ggcggggcaa tcccgtccct
     4501 gcccaccatt tcttgaaggg cttagccgtc gccgccttcg acctcgacgt gctgccgtgc
     4561 ccgcgggata atgacgggct catgccgatc tcggttctga tcgactgcga ccccggctac
     4621 gacgacgtga tcgcgatcat gctcgcgctg gcgtgccccg aggtggaagt gctcgggatc
     4681 acgaccgtcg aggggaacca gcccgtggcc gagaccacgg agaacgcgct gcgggtcgtg
     4741 gagttcctgg cccgcaagat tcccgtcgct gagggtgctg accggccgct cgtgcgtgag
     4801 ctccccttcc ggcggacgcc gagcacggac agcgccgacc tgcgacgcga catccccggg
     4861 ttgccgcctg cgacgggtcg agcggtggac gagcacgcgg tcgacctcct cgcgcgggtg
     4921 ttgctcgccg cggacgagcc ggtgacgctt gttccgctcg ggccgctcac gaacgtggcg
     4981 ctgctcctgg cgcggtaccc cgacgcggct aggcgcatcg ggcgcatggt cctgatgggt
     5041 ggcgcgatcg gtgagggcaa cgtcaccacg gccgccgagt tcaacatcta cgtcgatccc
     5101 gagtccgcgc agcgcgtctt ctccagcggg atcgacctca ccatggtggg cctcgacgtg
     5161 acgtaccggg caatcgtcag cccggcggag ctggaggagc tgcgcggcct cggccgcgcc
     5221 ggcagcgtcg tcgccgcgat cctcgacgcg tttccgcctt accgccgcta ctcgtggtat
     5281 ccggagggct acccgataca tgacgcggtc gcggtggccg agctcgtgca gccggggctc
     5341 ctgacgacgg agtatgtgaa cgtcgtggtg gagt
//
LOCUS       DASRJQ010000234        16686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3746395, whole genome shotgun sequence.
ACCESSION   DASRJQ010000234 DASRJQ010000000
VERSION     DASRJQ010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 16686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16686
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3746395"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VFB89_05245"
     CDS             complement(<1..346)
                     /locus_tag="VFB89_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26738.1"
                     /translation="MSHGRAWIWVCASLATVSPASAQVQVRLGDSARANYSELHEGLK
                     DGTPAADTVKALLSSRNTRRLWGRVTAAVNGDGSWNDALLAMTRLAILRDPQVVDSAL
                     RIRKYFEQAGPYP"
     gene            complement(343..1572)
                     /locus_tag="VFB89_05250"
     CDS             complement(343..1572)
                     /locus_tag="VFB89_05250"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZM26739.1"
                     /translation="MQATRTTTVLTDGTNVVPRTVKGLSLVSLFNDFASEMVYPLLPA
                     FVTGALGGGPVALGALDGAADLTAALLKWVSGWLADRPGWRRPLILIGYLTAVLIRPL
                     ISVAGAASQVIGFRVIDRIGKGLRTPPRDALIADVTPPAARGRAFGFHRGADHFGAIL
                     GSLAAWYFLSAGANVRQVIAWSALPGVVAFVVLAFVLARRSAKTGAAGARSPEHKNGL
                     TEAESPGQAGFWPPILALAALTFCRMPEALLLLRLQQAGVAVTLVPLVWAALHVVRSG
                     ASYPGGRLVDSVGPRIAVTAGAVLFALAIGAMGLETGAVGVTVAFLVFGGVAGLTESA
                     ERSLVARLAPVHIGRGFGAYHAVTGLAALPAAVVFGELYQQLGSQTALWGSAAAVLLA
                     AGWWLVVSPAGTAESTS"
     gene            1629..2663
                     /locus_tag="VFB89_05255"
     CDS             1629..2663
                     /locus_tag="VFB89_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26740.1"
                     /translation="MATPPGPFRESSLWSTPIRDLGLTIEGTRLEPILDEFKEELKRA
                     GLMRLEPRFYLSTEWGVAFQTVAIAIPFYLARPELTAIHQEHRGHIEGFDRADILRYL
                     RHEMGHVVNYAYRLYEDEEWILRFGSITQPYEEDYRPQPFSRRYVRHLPGWYAQKHPD
                     EDWSETFAVWLTPGLDWRREYEGWPEALAKLSYCERTVARILDRDPLVTDAEPDEDVA
                     EIPYSVREYYEDESAPPPEATFPPGLDGALRAIFERLGDGTGTGGNSFRRADGLIRSL
                     ERDLMENVFRWAGHFPERTRVLVRHLADRAGTLELGYEPSQESAAMLAVTTLVTALAM
                     NYVHRGSYLP"
     gene            complement(2660..3382)
                     /locus_tag="VFB89_05260"
     CDS             complement(2660..3382)
                     /locus_tag="VFB89_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26741.1"
                     /translation="MQPVAAVLLGLTTGLFGFSRQLAAQADARHGVEQQMAGPIAAGP
                     HLILTPTRIPTAADSSRAAAIVDTLRRAIAKYTDVHAAEADGFRLFAPRVKNQPVFHY
                     TRWLSAARAAYEFDPARPTSLLYRREANGSMRLIGAMYTAPQRFTPAELDRRIPLSIA
                     RWHLHTNMCIPPLRQRERWKETREGAPRFGPGSPIATKAACDDVGGRFLPKLFGWMVH
                     VNVFENDPASIWAMPHEDHHAG"
     gene            complement(3451..4464)
                     /locus_tag="VFB89_05265"
     CDS             complement(3451..4464)
                     /locus_tag="VFB89_05265"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26742.1"
                     /translation="MSDSVILQDVKITVLTYLDAPDENSKDYDPVVPQVARTLRRLGH
                     RVSVLGVHGDVKRLISGLSRRKPDLVFNLMEMFADNLFGDIPIAGMLDLIGVQYTGSG
                     PGELYLSQDKALSKKLLSYEDILYPRFAVFNRDSELETGGNLRMPLFVKPLRSDASLG
                     VSRKSLVHDALQLMKQVAFVRTELNDSAIAEEFIEGREFLVGVIGNSPPKALPPIEVD
                     FSGLPEGAPKVLDSKAKWEERSAEYKGTKTIVATLPDELRARLQKVAVDAYRAVRVRD
                     YGRVDLRLTDTGEIYVLEVNASCYLEKNSEFAMAAAAAGLDYPRLIERITNLAVERYN
                     GKR"
     gene            complement(4475..6061)
                     /locus_tag="VFB89_05270"
     CDS             complement(4475..6061)
                     /locus_tag="VFB89_05270"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HZM26743.1"
                     /translation="MRELDRLCESYGDLLQHLDPAAANAAGFVSGVAHLGRFDREAVR
                     EHAAALRATASAIEELEVEELADEIDRTALLDDVRVSLAQLEEDRAPIRHPQFWIDHL
                     GRTVASFLLPWNARPVGSGGSNGLADCIAGIPSFLDSARTTLRRPFHLLVDAALAQLV
                     SAGELLVHAATVAAEQAPVPGEHSEGSHASDAFVASDGINALVTEALRSLTAFGHWLR
                     SDVEPEPALANAVLGEARFERRIHHRHSVREGPAELWRYAEHLLENTEAALIAEARGA
                     GWRELVQAARESTVPAASTVSTELERVRNLVGEAGITVPAGPEVVVAPAYLRARVADA
                     TYVPSTSGPGRLVLPAAQLSRGAASAIAAAALAGRHVQESSARSLASLVRQTLRAPIA
                     VRGWALYAEEWMAERGLYVDAESRLFRLLHLLRAAGRLAVDVGFHARAMTLDDAMKLL
                     IERAGFTPEGAQGELQHCLAHPTDGAAAALGRREILTLRAKAGVPSSDARVLDRFHAE
                     LLQYGALPPGLAGWGLGLAG"
     gene            complement(6061..7047)
                     /locus_tag="VFB89_05275"
     CDS             complement(6061..7047)
                     /locus_tag="VFB89_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZM26744.1"
                     /translation="MQVIIPLAGKGTRLRPHTHLVPKPLLKVAGRPVMDWVMDRLEGL
                     QVEELIFITGHLKEQVEEYTRSRYPYPSRYIEQKVQDGTAGAVNLARPFVDGPVLIIF
                     VDTVFEADLTLINRVDADGIIWAKEVEDYQRFGVVVTDSQGFMSRIVEKPREPISRLA
                     NIGLYYIRAVDSLWQGIDYVLSSAPNKGEYYLTDAFQWMIEHGKRIFTAEVGGWYDCG
                     KLDTLLETNAILLNNGAARSASFPGVTIREPVYIEDGVKIERSTVGPNVSLERGTSVM
                     DSTLANAIVGRDTTLHNVSLDGALIGNSVLVEGLKGSATLGDHSEVTARPSS"
     gene            complement(7038..7403)
                     /locus_tag="VFB89_05280"
     CDS             complement(7038..7403)
                     /locus_tag="VFB89_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HZM26745.1"
                     /translation="MAEVPYRVEKPWGYELVWARTDKYVGKVLHVKAGHILSLQYHNF
                     KDETMHVLRGELVLRTKEGEQLVSRPFRAGETVHIPPKLIHQIEAVQDSDVLEASTPH
                     LDDLVRLEDRYGRGSDPCK"
     gene            complement(7403..7984)
                     /locus_tag="VFB89_05285"
     CDS             complement(7403..7984)
                     /locus_tag="VFB89_05285"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZM26746.1"
                     /translation="MSPMLPANSNRRDLLQSVFGEWLERLVAETERKIIQRRYVRPPG
                     ALPEVGFLTACTRCGACVTACPVQAIRTAGTDGGLAAGTPFLDPEHQPCVVCTDMPCA
                     RACPTGALSVPTRGWEGYRLTELEFLPENCVTFRGTECRVCVDACPVGEDALVIDGTG
                     HPSLRREGCVGCGICVRDCIARPKAFTLHYAEG"
     gene            8127..8579
                     /gene="tnpA"
                     /locus_tag="VFB89_05290"
     CDS             8127..8579
                     /gene="tnpA"
                     /locus_tag="VFB89_05290"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HZM26747.1"
                     /translation="MPGVYAKLRVHLIWGTRKRRPWLDPEWRGRLFARMDVLAAAIGA
                     RLVSAGAGRDHVHLYLELPATLALAPVVEAVKSGSARWIRRTFGHRNGFDWQAGYAAF
                     SVTPHDDARLLDDLRHQDVRHRDRDFAREYLALLELHQVPYELHDALD"
     gene            complement(8652..9188)
                     /gene="atpH"
                     /locus_tag="VFB89_05295"
     CDS             complement(8652..9188)
                     /gene="atpH"
                     /locus_tag="VFB89_05295"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HZM26748.1"
                     /translation="MRSETVARNYAETLFELGERTGKTELYAGLIDAVAGVVETTPAI
                     RDMLMSPRVPKAEKSRLLGAGLKDAPKQFVLFLQAVVRRGRGQLLREIANAYAALLDI
                     KFNRVRAGVMLVRTPDEALCRSIEAGLSKAFGKQVIATFSADPQLLGGAVVRVGERVY
                     DGSVRRRLTRLRRHLLSR"
     gene            complement(9185..9757)
                     /gene="atpF"
                     /locus_tag="VFB89_05300"
     CDS             complement(9185..9757)
                     /gene="atpF"
                     /locus_tag="VFB89_05300"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HZM26749.1"
                     /translation="MKRAFWLGVGAALFGAVPAVAEEGGPSGPLVVNGGLMIWTLVVF
                     ALLFLVLKRFAWPALLGAVEAREKRLEEQLAEAERNRAESARLLEEHKKLLLDTRTQA
                     SGLLAEARAAAEKERALALQKTKQEQEDLLERARREITAERERALVELRREAVDISLA
                     AASKLIGERLSSDSDRKLVDSYLATLEPRR"
     gene            complement(9768..10046)
                     /gene="atpE"
                     /locus_tag="VFB89_05305"
     CDS             complement(9768..10046)
                     /gene="atpE"
                     /locus_tag="VFB89_05305"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HZM26750.1"
                     /translation="MLHMLALLQDAAAATAGKGYGLIGAGLGAGLAVLGAGVGIGRIG
                     GSAAEGMARQPENSARIQTAGLIFAALIEGVALFVAVIALLMQGKITF"
     gene            complement(10316..11275)
                     /gene="atpB"
                     /locus_tag="VFB89_05310"
     CDS             complement(10316..11275)
                     /gene="atpB"
                     /locus_tag="VFB89_05310"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HZM26751.1"
                     /translation="MKWIGPSAMLVLLAATQLPAQEIVHPKVAFQAAQESHKQSPQEI
                     DIMGHLANSNEIELPYWKPPYYYALHLPRLKPVEVGSVSIDLSPTKHAVFAVIAALLV
                     AIIFIASARSVRRAQANGHPPKGLGGAMEATALWIRQEVILPNVGPHGEGYAPYLLTT
                     FFFILACNLLGLLPWGATATGNIAVTGGLAVLAFVIIEVSGMRSLGFKGYMGTIFYLP
                     PGLPTFLKPIMLIVMTPIEIIGKLAKPFALAVRLFANMTAGHALVLALIGLTFTFHSY
                     VVGGVASVMATLVMLLELFVAFLQAFVFTLLTAVFIGLMRAEH"
     gene            complement(11284..11649)
                     /locus_tag="VFB89_05315"
     CDS             complement(11284..11649)
                     /locus_tag="VFB89_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26752.1"
                     /translation="MKGARRYLAVTAALVSLGGAAGLVIPEVWRAGWWLALGLTLAIQ
                     GPLGWWLMASLGTERFLGVWALGIFTRLALVALVGLVLVPGLGLPPAPTLLSLVGFLM
                     VSLAIEGIVAVQCSRVEVR"
     gene            complement(11646..11876)
                     /locus_tag="VFB89_05320"
     CDS             complement(11646..11876)
                     /locus_tag="VFB89_05320"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HZM26753.1"
                     /translation="MSEPNKGGGGGPGPAKYAGLGIQFAVTILLFVLAGQWADRKMGT
                     DGLFTILGTFLAFGGTLYSLIRHLNKDNQDRR"
     gene            12003..13022
                     /locus_tag="VFB89_05325"
     CDS             12003..13022
                     /locus_tag="VFB89_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZM26754.1"
                     /translation="MTVTQAVPQDDVRQLERLVGAVTRLRSQVAQRIVGQDDIVEGIL
                     TAILAGGHALLIGVPGLAKTLMVQTIAEALRLSFNRVQFTPDLMPSDITGTEIIEEDL
                     TTGKRAFRFVHGPVFANVVLADEINRTPPKTQAALLQAMQEHQVTAGGTTYHLPDPFF
                     VLATQNPIEQEGTYPLPEAQLDRFMLELRVNYPSRNEEEAIVEQTTGAVRGSLESVLD
                     AEAVLAMQALVRRIPVSRSLIRAAVNLARLTRPADPEAPAFIKEYVEWGAGPRASQYL
                     VLGAKARAAIAGRPMADLDDVKGVAPAVLRHRIVTNFAAEAADRTGADLVGELLSGKA
                     WSVGL"
     gene            13108..14244
                     /locus_tag="VFB89_05330"
     CDS             13108..14244
                     /locus_tag="VFB89_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26755.1"
                     /translation="MTLIAKRVMALAVAVCATVLAGCGGDPVSSAPAAIADPVGLNND
                     VASLSTPLQGSVFQSFSTLGSTSPISGRTTTVLSAMTPSRLALLSRQGVTPKQALALK
                     SLRPSFATAPTGAGSLIPETQWGKVYVWDTGTHAYVEGSATGGPATGVRFTLYAIDPL
                     TNQPATPLNAIGYADLTDESTDTDYRLGVLVASETATYADYLVTATSTQTSFQAVAAG
                     YVTDGAHRLDFNNSASASSNHIAVDFDLTLDHPDVAAHLHADLTAGDPTTVLSLTLSV
                     TRGGETVALAGTVGFTTSQSGASAVANLVVTVNGRQFATITGTVTSNSAAGYTFTGPD
                     RDLTAQERNALNQMMAAPAVLTAAAAPMFTAAEQLIGSSYSLGL"
     gene            14439..14894
                     /locus_tag="VFB89_05335"
     CDS             14439..14894
                     /locus_tag="VFB89_05335"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HZM26756.1"
                     /translation="MRTMIAAAGLAVMALAGAASSASAQDADQNKTLFTVVVNQVYNG
                     GDLALIDDLVSKNVSHNGTPLGREGYKALVKDERTKDPSRKLTVNELVTDGDRVIGRL
                     SSSTGSGEILVLRIQDGQVTEQWTMADEPALRKQFGLKENPGQSGTSAN"
     gene            complement(14936..16357)
                     /locus_tag="VFB89_05340"
     CDS             complement(14936..16357)
                     /locus_tag="VFB89_05340"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZM26757.1"
                     /translation="MARTSMILQIPGSPIPGPLVATAPPALSDRPLSTDSDAKTVGPN
                     SFVRWKILALLALASGVAYVLRQNMSVAGERMMSDLGLSQVQLGIVLGAFAWAYGIFQ
                     FPGGVFGDWLGARKGLSLIIAAWGVLNLLVALIPGTAGTSPVMIIALLAALRFLMGVA
                     QAPLFPILSGHSIARWFPVGAWAFPNAVTNAGLTLGSAATGPLIAWLAHTLGWRASFA
                     TTAPLAFLMAAAWWWYSRDQPAQHAGVSARELAHINAGRPDFRAPANPRREWRLVLRD
                     RNLLLITASYFAANYVFYFFFNWLYIYLVDVRKFQVLTGGLFASAPWICGAIGATLGG
                     MICDRLARRHNMTISCRVVTICGLVLAGVFIVAAAGAASPYVALIFLCLCLAAQQFTD
                     AASWAAATMVGGSRAAMTCGVMNTGGNVVGGLVAVVVPITAREFGWPVAVGSASLFAF
                     VGAVLWLWIRVSPPTNEGRPPGP"
     gene            16459..>16686
                     /locus_tag="VFB89_05345"
     CDS             16459..>16686
                     /locus_tag="VFB89_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26758.1"
                     /translation="MDGQLFLRTLGVPALFAPNGDPIRFRVKKHVALLVYLAVEQRTQ
                     RRDRLAELLWPRVSATEARHSLATALSVLRPR"
BASE COUNT         3082 a         5601 c         5213 g         2790 t
ORIGIN      
        1 aggggtaggg accggcctgc tcgaagtact tgcggattcg cagcgcgctg tcgaccacct
       61 gcggatcccg caagattgcg agccgggtca tggcgagcag ggcatcgttc caggagccgt
      121 cgccgttcac tgcagcggtc acccggcccc atagccggcg agtatttcgc gagctgagca
      181 gcgccttaac ggtgtctgct gccggagtgc cgtccttcag tccctcgtgc aactccgagt
      241 agttggcccg cgcggagtcg ccgagacgca cttgcacctg ggcggaagcc ggcgacacgg
      301 tcgccagcga agcacagacc cagatccaag ccctcccatg gctcaacttg tcgactccgc
      361 agtgccggcc ggtgacacga cgagccacca gcctgctgcc agcagcaccg ccgcggcaga
      421 cccccagagg gcggtctgcg agcccaattg ctgatacagt tcgccgaaca cgacggcggc
      481 cggcagcgct gccagcccgg tcaccgcgtg gtatgccccg aatccgcgcc caatgtgaac
      541 cggcgcaagg cgagcgacca gcgatcgttc cgccgactcg gtcagacccg caacaccgcc
      601 gaagaccaga aaggccaccg tgaccccgac cgctccggtt tcgagcccca tcgcgccgat
      661 ggccagggca aacaggaccg caccggcagt cacggctatg cgcggtccca ccgaatcgac
      721 caaccgtccg ccggggtagg aggcaccact acgcaccaca tgcaacgcag cccagaccaa
      781 tggcaccagg gtcacggcca cccccgcctg ctgcagccgt agcaatagca gcgcttccgg
      841 catccggcaa aaggtgagcg cagcaagcgc gagaatcgga ggccagaatc ctgcttgccc
      901 aggcgactca gcctctgtaa gcccattctt gtgctcgggc gatcgggctc ccgccgcgcc
      961 cgtctttgcc gaccgtctcg cgagaacgaa ggccaatacg acgaacgcga ccacgcccgg
     1021 cagggcgctc cacgcgatta cctgccgcac gttggcgcca gccgacagga agtaccatgc
     1081 agccagtgag cccaggatag ccccgaagtg atcggctccg cgatgaaatc cgaaggcgcg
     1141 cccacgggca gccggtggtg tcacgtccgc aatgagtgcg tccctgggag gggtgcgcag
     1201 acccttcccg atccggtcga tgaccctgaa cccgatcacc tgcgaggcag caccggccac
     1261 cgaaatgagt ggcctgatca gcacagcggt gagatatccg atgaggatga gcggacgccg
     1321 ccagcccggt cggtcggcca gccacccact gacccacttg agcagggcgg ccgtgaggtc
     1381 ggccgctccg tccaacgccc ccagcgccac cggtccgcca cccaaggcac cggtgacgaa
     1441 cgccggcagc agcgggtaga ccatctcgct cgcgaagtcg ttgaacagcg agacgaggga
     1501 cagacccttc acggtgcgcg gaaccacgtt cgttccgtcg gtcaggacag tggtggtgcg
     1561 ggtggcttgc atggcggtgg aatatatgtt tgacgggttc cgctccagct ccgatcaccg
     1621 agagactcat ggcaaccccg ccgggcccgt ttcgcgaatc cagtctgtgg tcgaccccca
     1681 tccgcgacct cggcctgacc atcgagggca cccgcctgga gcccattctc gatgaattca
     1741 aggaggagct gaagcgcgcc gggctgatgc ggctcgagcc ccgattctac ctgtccaccg
     1801 agtggggtgt ggcattccag acggtcgcca ttgcgatccc gttctatctg gcgcgccccg
     1861 agctcacggc gattcaccag gagcaccggg gccacatcga aggcttcgat cgggccgaca
     1921 tcctgcgcta cctccgccat gagatggggc acgtcgtcaa ctacgcatac cggctgtatg
     1981 aggatgagga gtggatcctc cgcttcggct ccataactca gccctacgaa gaggactacc
     2041 gtccccagcc cttcagccgc cgctatgtcc gccaccttcc cggctggtac gcccagaagc
     2101 atcccgatga ggattggtcg gagacattcg cggtctggct gacgcccggt ctcgactggc
     2161 gacgggaata cgaaggctgg cccgaggcgc tcgccaagct ctcctattgc gagcgcacgg
     2221 tggcccgaat cctcgatcgc gacccgctgg tcacggacgc cgagcccgac gaagacgtgg
     2281 ccgaaattcc ctattcggtg cgcgagtact acgaggacga atcagctcct ccaccggagg
     2341 ccaccttccc cccgggcctc gacggcgcgc ttcgcgccat cttcgagcgg ctgggggacg
     2401 gcaccggcac cggcggtaac tcctttcgcc gagccgacgg gctcattcgt tcgctggagc
     2461 gggacttgat ggagaacgtg ttccggtggg cgggacattt cccggaacgt acccgcgtgc
     2521 tggtgcgcca cctcgctgac cgagctggga cactggagct ggggtatgag ccgtctcagg
     2581 aaagcgcggc catgctggca gtgacgaccc tggtcactgc actggccatg aactacgtgc
     2641 accgcggcag ctacttgcct tagcccgcgt ggtgatcttc gtgcggcatc gcccagatcg
     2701 acgctggatc gttttcgaac acattcacgt gcaccatcca cccgaagagc ttgggcagga
     2761 accgtccacc gacgtcgtcg caggcggcct tggtcgcaat cgggctcccg gggccaaacc
     2821 gcggcgctcc ttcacgagtc tccttccatc gctcacgctg acgcagtggc gggatgcaca
     2881 tgttggtgtg cagatgccag cgagcgatcg ataacggaat gcgccgatcg agctctgcgg
     2941 gtgtgaagcg ctgcggggcc gtgtacatcg ccccgatgag ccgcatgctg ccgttcgctt
     3001 cccgacgata gaggagcgaa gtcggtcgag ccggatcgaa ctcgtaggca gcgcgggctg
     3061 ccgaaagcca ccgggtgtag tggaagaccg gctgattctt gactcgtggt gcgaacagcc
     3121 ggaagccatc ggcctctgcc gcgtgaacgt cggtgtattt cgcgatggct cgtctcaatg
     3181 tgtcgacgat ggcggctgct cggctggagt cggccgccgt ggggattctt gttggcgtca
     3241 ggatgaggtg ggggccggcc gcgatagggc cggccatctg ctgctccacg ccatgccgcg
     3301 cgtcggcctg ggcggccaac tgtcgactaa agccgaatag cccggtggtc agcccgagaa
     3361 ggacggcggc gacaggttgc aaagaggaaa tcgaatgcga cattgccgac gctgacggcc
     3421 tgtcccgcgc ggcgttaagg cgaaggcttt ctatcgcttc ccgttgtagc gctccaccgc
     3481 gaggttcgtg atacgctcga tcagccgggg atagtcgagt ccggccgcgg ccgcagccat
     3541 ggcgaactcg ctgttcttct ccagataaca gctggcattc acctcgagca catagatctc
     3601 acctgtgtcg gtgagcctga ggtccacccg gccatagtcc cgcacccgca cggcacgata
     3661 ggcgtcgacc gctaccttct ggagccgtgc ccgcagctcg tcgggcagcg tcgcgacgat
     3721 ggtcttggtt cccttgtact cggcgcttcg ctcttcccat ttcgctttgc tgtcgagcac
     3781 cttgggtgcg ccttcgggca gcccgctgaa atccacttcg atcggcggca acgccttcgg
     3841 cggcgagttc ccgatcacgc ccaccaggaa ttcgcggcct tcaatgaact cttcggcgat
     3901 ggctgaatcg ttgagctcgg tgcggacgaa ggccacctgc ttcatcagtt gcagcgcgtc
     3961 atgaaccaat gatttccggc tcacgccgag cgaggcatcg gagcggagcg gcttgacgaa
     4021 gagtggcatc ctgaggttgc cgccggtctc aagctccgag tccctattga acaccgcgaa
     4081 tctcggatag aggatgtctt cgtaggagag cagcttcttg ctgagcgcct tatcctggct
     4141 caggtacagc tcccctgggc cgctgccggt gtactgcacg ccgatcaggt cgagcatgcc
     4201 ggcgatcgga atgtcgccga agagattgtc ggcgaacatt tccatcagat tgaaaacgag
     4261 atcgggcttg cggcgggaca gcccactgat gagtcgtttg acgtcgccgt ggacgccgag
     4321 gaccgaaacg cggtggccga gacgtcgcag tgtgcgagcg acttgcggca caaccggatc
     4381 gtagtccttg gagttctcgt ccggcgcgtc gagatacgtg aggaccgtga tcttcacatc
     4441 ctgaagaatg acggaatcgc tcacgcaatg ctagtcatcc agcgagtccg agtccccagc
     4501 ctgccagccc cggaggcagt gcgccgtatt gcaacagctc cgcatgaaag cgatcgagca
     4561 ctcgtgcatc gctcgagggg acacccgcct tcgctctgag cgtgagaatt tcacgccggc
     4621 ccaaggccgc tgctgcaccg tcggtaggat gcgccaggca gtgttgcagc tcgccttgtg
     4681 cgccttcggg cgtgaagccc gcgcgctcga tcaacaactt catcgcatcg tcgagggtca
     4741 tggcgcgagc gtgaaaccct acatccaccg cgagtcggcc ggctgctcgc agaaggtgga
     4801 gcaggcgaaa caggcgcgat tcggcatcca cgtagagccc ccgctccgcc atccattcct
     4861 ccgcgtagag cgcccaacct cgaaccgcga tcggagcgcg tagtgtctga cggaccaagc
     4921 tcgccagcga tcgcgcactg ctttcctgaa cgtggcggcc cgccagcgca gcggccgcaa
     4981 tggccgacgc tgcgccgcgg ctcaactgcg ccgccggcag cacgagtcgc ccagggcctg
     5041 aggtcgatgg cacgtaggtg gcgtcggcca cgcgtgcacg aaggtaggct ggtgcaacca
     5101 ccacttccgg tcccgcagga acggtgatcc cagcctcgcc gaccagattg cgtacccgct
     5161 cgagctcggt gctcaccgtc gaagccgcgg gtacggtgct ttcccgggcc gcctgcacca
     5221 gctcacgcca gcctgcgccg cgcgcttcgg cgatcaacgc ggcttcggtg ttctcgagca
     5281 ggtgctccgc gtagcgccac agctcggccg gaccctcccg aacagaatgg cggtggtgaa
     5341 tccgtcgctc gaatcgcgct tcgccaagaa ctgcgtttgc gagtgcaggc tccggctcca
     5401 catctgagcg gagccaatgg ccgaaggcgg ttagcgagcg cagcgcttcg gttaccaatg
     5461 cattgatccc atcggacgcg acgaaggcat cagatgcgtg gctcccctcg gaatgctccc
     5521 ccggcacggg agcctgttcg gctgccaccg tcgccgcgtg gaccagcaac tctcctgcgc
     5581 tgacgagctg tgccagggca gcgtcaacca gcaggtggaa cggccggcgg agtgtagtgc
     5641 gcgcggaatc gagaaaggaa ggaatgccgg caatgcaatc ggccaggcca ttgcttccac
     5701 cggagccaac cggcctcgcg ttccagggaa gcagaaagga ggccacggtc cgacccaggt
     5761 gatcgatcca gaattgggga tgccggatcg gggcccgatc ctcttccagc tgggccagac
     5821 tgacccgaac gtcatcgagc agcgcggtcc ggtcgatttc atcggcaagc tcctcgactt
     5881 cgagctcttc gattgccgac gcggtggctc gaagcgcggc ggcgtgctcc cggacggcct
     5941 cacgatcgaa gcgacccaaa tgagccaccc ccgaaacgaa tccggcggcg ttcgccgccg
     6001 ccggatcgag atgctgaagc aaatcgccgt acgactcgca caaccggtcc agctcgcgca
     6061 tcaggaagac ggccttgccg taacctcgga gtggtcgccc agagtggcgc tccctttgag
     6121 cccttcgacg agcacgctgt tgccgatgag cgcaccgtcc aggcttacgt tatggagcgt
     6181 cgtatcgcgt cctacaatgg catttgcgag tgtgctgtcc atcacgctgg ttcctcgctc
     6241 cagcgaaaca ttggggccca cggtactccg ctcgatcttg acgccgtcct cgatgtagac
     6301 cggctcccga atggtcaccc ccggaaacga ggcgctgcgg gccgcaccgt tattaaggag
     6361 gatcgcgttg gtctcgagca gcgtgtccag cttaccgcag tcgtaccagc cacccacttc
     6421 ggcagtgaag atccgcttcc catgctcgat catccactgg aaggcatccg tgaggtaata
     6481 ctctcccttg ttcggcgcac tggagaggac gtaatcaatg ccctgccaga ggctgtccac
     6541 cgctcggatg tagtacagtc cgatgttggc caggcgtgag attggttctc ttggcttttc
     6601 cacgatccgg ctcatgaagc cctgcgagtc ggtcaccacg acgccgaacc gctgataatc
     6661 ctcgacctct ttggcccaga tgatcccgtc ggcgtccact cgattgatca gggtcaggtc
     6721 ggcttcgaac accgtatcga cgaagatgat cagcactggc ccgtcgacga acggccgagc
     6781 caggttgact gcaccggcgg tcccgtcctg caccttctgc tcgatgtagc gggaggggta
     6841 gggatagcgt gatcgagtgt actcctcgac ctgctccttg aggtgtccgg tgatgaaaat
     6901 cagctcttcc acctgcaggc cttcgagccg atccatcacc cagtccatca cgggccggcc
     6961 cgcgaccttg agcaggggtt tcgggacgag atgagtgtgc gggcggagtc gagtgccttt
     7021 gcccgcgagc ggaatgatca cttgcacggg tcagaccctc ggccgtaccg gtcttccaag
     7081 cgaaccagat cgtccaggtg aggtgtggac gcctccagca cgtcgctgtc ctgtaccgcc
     7141 tctatctggt ggatcaactt gggagggatg tgcaccgttt ccccggctcg aaacggtcgc
     7201 gacaccagct gctcgccttc cttggtccgc agcacgagtt cgcctcggag tacgtgcatg
     7261 gtctcgtctt tgaagttgtg gtattgcagg ctcaggatgt ggcccgcctt cacgtgcagc
     7321 accttgccga cgtacttgtc ggtgcgcgcc cacaccagct cataccccca cggcttctcc
     7381 acccggtacg gcacctcggc catcagccct ccgcgtagtg cagcgtaaat gccttcgggc
     7441 gcgcgatgca gtcgcgcacg cagatcccac agccgacgca accttctcgc ctcagcgacg
     7501 gatgtccggt tccatcgatc acgagcgcgt cctcaccgac tggacaggcg tcgacgcata
     7561 cccggcactc ggttccccgg aacgtgacgc agttctcggg aagaaattcg agctcggtca
     7621 gccggtagcc ttcccaaccg cgggtgggga cgcttagcgc accggtggga cacgcccggg
     7681 cgcagggcat gtccgtgcac accacgcagg gctggtgctc cggatcgagg aacggtgtgc
     7741 cggctgcgag tccaccgtcg gtacccgcgg tccgtattgc ctgaacgggg catgcagtaa
     7801 cacacgctcc gcaacgggtg catgcagtaa ggaagccgac ttcgggcagc gcgccgggcg
     7861 gccgcacgta gcgccgctga atgatcttcc gctccgtctc cgcgaccagc cgctcgagcc
     7921 actccccgaa cacggactgc agcaggtccc tgcgattgga gttggcgggc agcatcggcg
     7981 acatcacggg aaagcgtggg gcgaaagata ggcggggaac gggcggacgc gcagtgagcc
     8041 cggccgtcga gactccacga gcgaatccgc cacggaccgc acacccgtga tggcataacg
     8101 gcgggccgcc gcactcatga tccgccatgc caggcgtgta tgcaaagttg cgggttcacc
     8161 tgatctgggg aacgcgaaag cgccggccgt ggctcgaccc tgaatggcga ggacgcttgt
     8221 tcgcccgaat ggacgttctc gccgcagcca tcggtgctcg gctggtgtcg gcgggcgcgg
     8281 gccgtgacca cgtgcatctc tatctcgagc tgccggccac tctggcactt gccccagtcg
     8341 tggaagccgt gaagagcggc agcgcccgct ggatccggcg cacctttggc catcggaatg
     8401 ggttcgattg gcaggccggg tacgccgcat tcagcgtaac cccgcatgac gacgcccggt
     8461 tgctcgacga cttgcgacat caggacgtgc gtcatcgaga ccgggacttc gctcgggagt
     8521 acctggctct actcgagctg caccaggttc cctacgagct gcacgacgcg ctcgactgat
     8581 cgtcaagcgt cgcaagaacg caaatgggcc cgctcggaag ccgggcccat ttgcttcacc
     8641 gtttgcgcga actagcgaga gagcaagtgc ctccgaagcc tggtcaggcg gcggcgaact
     8701 gaaccgtcat acacccgctc gcccacccgg accacggccc cgccaaggag ctgcggatcc
     8761 gcgctgaatg tggcaatcac ctgcttgccg aacgcctttg acagccccgc ctcgatgctt
     8821 cggcacaatg cctcgtccgg tgtccgcacc aacatcacac ccgcgcgaac gcgattgaac
     8881 ttgatatcga gcagcgccgc gtacgcgttg gcaatttccc gcagcagctg accgcgtccg
     8941 cggcgcacca ctgcctggag gaacaacacg aactgcttgg gtgcatcctt gagaccggct
     9001 cccagcaatc ggctcttctc ggccttgggc acccggggcg acatgagcat gtccctgatc
     9061 gccggtgtcg tctcgaccac acccgccacc gcgtcgatca gcccggcata gagctcggtc
     9121 tttcccgtcc gctctccgag ctcgaacagc gtttccgcgt agttccgggc caccgtctcc
     9181 gatctcaccg gcgtggctcc agggtcgcca ggtaggaatc gaccaacttg cggtcgctgt
     9241 cactgctcag tcgttcgccg atcagcttcg acgcggcggc gagagagata tccaccgcct
     9301 cgcgccgcag ctccaccagc gcccgttccc gctcggccgt gatctcccgg cgggcgcgct
     9361 ccagaagatc ttcctgctcc tgtttggtct tttgcaacgc gagcgcccgc tccttttcgg
     9421 cagcggcgcg tgcctcggcc aaaagaccgc tggcctgagt ccgcgtgtcg agcaggagct
     9481 ttttgtgctc ctccagcagc cgggccgatt ccgcccgatt tcgctcagcc tccgcgagct
     9541 gttcctccaa ccgcttttcc cgcgcttcga ctgcgcccaa cagggcgggc caggcaaagc
     9601 gcttcaaaac gaggaagagc agggcaaaaa cgaccagggt ccagatcatg agcccgccgt
     9661 tcactaccag cgggccgctg ggcccaccct cttcggcgac agccgggacg gcgccgaata
     9721 gcgccgcccc aactccaagc cagaacgctc tcttcatacc ggggtcctca gaacgtaatc
     9781 ttaccctgca tcagcagcgc gataaccgcg acgaacaggg cgacgccttc gatcagcgca
     9841 gcgaagatca gacccgccgt ctggattcgc gcgctgttct ccggctgccg ggccattcct
     9901 tcggccgctg agccaccgat gcgaccgatc cccacacccg cgccaagtac ggccagaccg
     9961 gcgcccaatc cggcaccgat gaggccataa cctttgccgg ccgtggcagc cgcggcgtcc
    10021 tgcagcaggg cgagcatgtg caacatgtca gactccatgg tagacaggag atccgttgta
    10081 gccgcgcccg ccgcacgtga gccgattctc ccggaccggc ttcgccacgg tgacccgcca
    10141 acgccctcgg ctcatgtcag agccgcgagc agcggatatc gtccgccatt cggcggacta
    10201 cggtgccgga actgcgcgag cgctcgcagc ccggcttgga gctatcagtt atcagctatc
    10261 agctatcgat cgcccctctt cccccttccc ccctcttctc cctttctccc gccgttcagt
    10321 gctccgcccg catcaatcct ataaacacgg ccgtgagcag cgtgaacacg aacgcctgca
    10381 ggaacgcgac aaacagctcg agcagcatca ccagtgtcgc catgaccgag gccacgccgc
    10441 ccactacgta gctatgaaat gtgaaggtca gcccgatgag cgccagcacc agcgcgtgcc
    10501 ccgccgtcat gttcgcgaag agtcggaccg cgagcgcgaa tggcttggcc agctttccga
    10561 ttatctcgat gggcgtcatc acgatcagca tgatcggctt gagaaacgtc ggaagccccg
    10621 gcggcaaata gaagatcgtg cccatgtaac ccttgaagcc aagggaccgc atgccggaga
    10681 cctcgatgat gacgaacgca agcacggcga ggccgccggt cactgcgatg ttgccggtgg
    10741 cggtggcgcc ccagggcagc agcccaagga ggttgcaggc caggatgaaa aagaaggtcg
    10801 tcagcagata aggcgcatag ccctcaccat gcgggccgac attgggcagg atcacttcct
    10861 gcctgatcca cagcgccgtc gcctccatag cgccgcccaa gcctttgggc gggtgcccgt
    10921 tggcttgagc gcgcctgacc gatcgcgcgc tggcaatgaa gatgatggcg acgagtagtg
    10981 ccgcgattac cgcaaagacc gcgtgtttcg tgggcgacag atcgatcgaa accgacccga
    11041 cctccacagg cttcagtcgt ggcagatgga gcgcgtagta gtagggcggt ttccagtacg
    11101 gcaactcgat ctcgttcgag ttcgccaggt gccccataat gtcgatttct tgcggacttt
    11161 gcttatgcga ctcttgcgca gcctggaatg cgactttagg gtggacaatc tcctgagcgg
    11221 gaagctgagt cgccgcgagt agcaccagca tggcactcgg cccgatccat ttcatcattc
    11281 ggctcatcgc acttcgaccc tcgagcattg gaccgcgacg atcccttcga ttgcgagtga
    11341 gaccatcaga aatccgacca atgagagcaa agtgggcgcg ggcggcaggc cgagcccggg
    11401 aaccagcacc aggccgacga gcgcgacgag cgcgagtcgc gtgaagattc ccagcgccca
    11461 gacaccgaga aatcgctcgg tccccagcga agccatgagc caccaaccaa gcgggccttg
    11521 aatcgcgagg gtcaggccca aggcgagcca ccaccctgct ctccaaactt ccggaatcac
    11581 cagtccagcg gcaccaccca gggatacgag cgctgcggtc accgcgaggt atcgccgtgc
    11641 tcccttcacc gccggtcctg attgtccttg ttcaggtggc gaatcagcga atacaacgtc
    11701 ccgccgaacg cgaggaaggt gccgagtatc gtgaacagac cgtcggtgcc catcttgcga
    11761 tccgcccatt ggcccgccag cacaaacagc aggatcgtga ccgcaaactg aatccccaag
    11821 ccggcatact tcgccggtcc gggccctcca ccgcccccct tgttgggctc cgacacggcg
    11881 ctaacctacg cccttgtgaa aagtttcgca agcgaacaaa ggccctcgat ctggtgctac
    11941 aatgctggcg tcttcgaccg gtccccggat aatgttgcag agtcccgtaa gccaaggcgc
    12001 gaatgactgt gacgcaggcc gttcctcagg atgatgtgcg ccagctcgag cggttggtcg
    12061 gtgccgttac ccgacttcgc agccaggtgg cgcagcgaat cgtcggacag gacgacatcg
    12121 ttgagggcat tctcaccgct attctcgccg gtggacacgc gctgctcatc ggggtgccgg
    12181 gtctggctaa gacactgatg gtccagacca tcgccgaggc gctcagactt tccttcaacc
    12241 gggtccagtt tactcctgat ctaatgccga gcgacatcac cggcaccgag atcatcgagg
    12301 aggatctgac tacagggaaa cgggcgtttc gcttcgtcca cggtccggtc ttcgcgaatg
    12361 tcgtactggc cgacgaaatc aatcgcacac cgcccaagac tcaggccgcg ctgctccagg
    12421 cgatgcagga gcaccaggtg accgctgggg ggacaacata ccacctcccc gatccgttct
    12481 tcgtgctcgc gacccagaac ccgatcgagc aggagggtac ctatccgctg ccggaagcgc
    12541 agctcgaccg cttcatgctc gagctgcggg tgaattatcc gagtcgaaac gaagaagaag
    12601 cgatcgtgga gcaaaccacg ggggccgttc gtggaagcct ggaatccgtg ctggatgccg
    12661 aggccgtgtt ggcgatgcag gcgctggtgc gtcggatccc cgtctcgcgg agtctgatcc
    12721 gcgcagccgt aaacctcgcc cgtcttactc ggccggccga cccggaggcg cccgctttca
    12781 ttaaggagta cgtcgagtgg ggcgccgggc cacgcgcctc tcaatacctc gtcctcggcg
    12841 ccaaggctcg cgccgcgatc gcgggccggc ccatggcaga cctggacgac gtaaaggggg
    12901 tcgcgcccgc tgtgctccga catcgaatcg tgaccaactt cgcggccgaa gccgccgacc
    12961 gaaccggtgc ggacctggtc ggggaactcc tctccggaaa ggcctggtcc gttggcctct
    13021 gacttgccct cctccccagc gcctggtttt ggccgtagca gctatctcac gccctgacaa
    13081 cagattacca gcccggagag gccccagatg actctgatcg ccaagcgcgt catggcgctg
    13141 gccgttgcgg tttgcgcgac cgtattggcc ggctgtggcg gcgaccccgt cagttccgcc
    13201 cccgcagcga tcgccgatcc cgtggggctc aacaacgacg tagcgtccct cagcaccccg
    13261 ctccagggct ccgtttttca gagcttctcg acgctcggat cgacgagccc catttcgggt
    13321 cgaaccacga ccgtgctgag cgcgatgacg ccgagccggc tggctcttct gagtcgtcag
    13381 ggcgttacac cgaagcaagc gctcgcgctt aagtcgctgc gtccgtcgtt cgcgacggcg
    13441 ccgaccggcg cgggctcgct cattccagaa acacagtggg gcaaggtcta cgtatgggac
    13501 accggcactc acgcgtacgt ggaaggcagc gccacgggcg gtcccgcgac cggcgtgcgg
    13561 ttcaccctct acgccatcga cccgctcacc aaccagccgg ccacgccgct caatgcgatc
    13621 gggtacgccg atctgaccga cgagagcacc gataccgatt atcgactcgg cgtcctggtg
    13681 gcgagtgaga cggcgaccta cgccgactac ctggtcactg cgacgtcgac ccagacctcg
    13741 tttcaggcgg tcgccgccgg atacgtgaca gatggtgccc accggctcga cttcaataac
    13801 tcggcaagcg caagctcgaa ccatatcgcg gtcgatttcg acctgacgct cgatcatccc
    13861 gacgtggcgg cgcacctgca cgcggacctc acggcaggag atccgaccac ggtcctcagc
    13921 ctcacgctta gcgtgacgcg gggaggcgag accgtggcgc tggccggcac cgtgggcttc
    13981 accacgagtc agtccggagc gagcgcggtg gccaatctcg tcgtcacggt gaacggaagg
    14041 cagttcgcga ccatcaccgg cacggtgacg tcgaactcgg cggcgggcta taccttcacc
    14101 ggccccgacc gcgacctgac cgcacaggag cgtaacgctc tcaaccagat gatggcggcg
    14161 ccggcggtcc tcacggcagc agcggcgccg atgttcaccg cagcggagca gctgatcggg
    14221 agcagctact cgctgggcct gtagaaccgg cgggggcggg cgggtcgagg gcgcgccggg
    14281 agtccggcgc gccctcgtgt ttcccggctg acgctcgtta cgaagccgta acccggagcc
    14341 caaaaccttc tgcgcaacct tcccgtatgg tggaggcgtc tataagccat gcggccccgt
    14401 tccggggtcg acgccattca ccggaaggag taagcgcgat gaggaccatg atcgctgcgg
    14461 ccgggctggc cgtcatggcc ctggcaggcg ccgccagcag cgcgtcggcc caggatgccg
    14521 atcagaacaa gaccctcttc acagtcgtgg tcaatcaggt ctacaacggc ggcgatctcg
    14581 ccctgataga cgacctcgta tcaaagaacg tgagccacaa cggtacgccg ctcgggcgcg
    14641 agggctacaa ggcgttggtc aaggacgagc ggaccaagga tccatcaagg aagctcacgg
    14701 tgaacgagct cgtcaccgac ggcgatcggg tgatcggccg gctctcgtcc tcgaccggaa
    14761 gcggagagat tctcgtgttg cggattcagg atggccaggt cacggagcag tggaccatgg
    14821 ctgatgagcc ggccctgcgg aagcagttcg gcctcaagga gaatccgggg cagagcggga
    14881 cgtcggcgaa ctagttcgca cccggtttga ccgcaacagt tgccgtcctc ccggctcacg
    14941 ggccgggagg gcggccttcg ttcgtgggcg gcgagaccct gatccagagc cacaggaccg
    15001 cgccgacaaa ggcgaagagc gacgcgcttc ccacggcgac cggccaaccg aactcacgag
    15061 ccgtgatcgg cactaccacc gcgaccagcc caccgaccac gtttcccccg gtgttcatga
    15121 cgccgcaggt catcgcagcc cgcgagccgc cgaccattgt tgccgcggcc caggaggctg
    15181 cgtcggtgaa ctgttgcgcg gcaaggcaca ggcaaaggaa tatcagggcg acgtatggac
    15241 tcgccgcgcc cgccgcggcg acgatgaaca cgcctgccag aaccagtccg cagatcgtga
    15301 ccacccggca gctgatggtc atgttgtggc gccgcgcgag tcggtcgcag atcatgccgc
    15361 ccagggttgc ccctatcgcg ccacagatcc agggcgcaga ggcaaacaag ccgccggtca
    15421 gcacctggaa cttccgcacg tcgaccaggt agatgtagag ccaattgaag aagaagtaga
    15481 acacgtagtt agcggcgaag taactcgcgg tgatgagcag gaggttgcga tcgcggagaa
    15541 ccagcctcca ctcgcgacgc gggtttgctg gtgctctgaa gtccgggcgt ccggcgttga
    15601 tgtgcgcgag ctcccgagcg ctcacgcccg cgtgctgggc cggctgatcg cgcgagtacc
    15661 accaccaagc cgcggccatg aggaaggcca atggcgcggt ggtcgcgaac gaggcgcgcc
    15721 agcccagcgt gtgggccagc cacgcgatga gcgggccagt ggccgcgctc ccgagagtga
    15781 gcccggcgtt ggtgaccgcg ttggggaaag cccaggcccc caccgggaac caccgggcga
    15841 tcgagtgccc gctcagaatg gggaagagcg gagcttgcgc gacgcccatg aggaatcgca
    15901 gggccgccag cagcgcgatg atcatgaccg gcgaagtccc ggcggttccg ggaatcaggg
    15961 cgacgagcag gttcaggacg ccccaggcgg cgatgatgag agacaggcct ttccgggcgc
    16021 caagccagtc gccgaatacc ccgcccggga actggaagat cccgtaggcc caggcgaacg
    16081 cccccagcac gatgcccagc tgcacctggg acaaccccag gtcgctcatc atccgctctc
    16141 cagcgaccga catgttctgc cgcagcacgt acgcgacccc gctcgccaat gcgaggagcg
    16201 caaggatctt ccatcgtacg aagctgttcg gtccaaccgt ctttgcgtcg ctgtcggtcg
    16261 acagcgggcg atcggagagc gccggaggcg cagtggccac gaggggacct ggaatcgggg
    16321 agcctggaat ctgcaggatc atggacgtcc tcgccaccac agcatcagga gcgtctagcc
    16381 gaggtcgcga aatcgacgca agtttgacgg tcggttgagg ccggcgctct actctcgatt
    16441 cacccccacg acaagctgat ggacgggcag ctctttctcc gcaccctggg cgtgccggcg
    16501 ttgttcgcac cgaacggcga tcccattcgg ttccgcgtga agaagcacgt ggcgctcttg
    16561 gtgtatctcg ccgtggagca gcggacccag cgccgcgacc gtctggcgga gcttctgtgg
    16621 ccccgagtct ccgccaccga ggcccgccat tccctagcga cggcgctctc ggtgttgcgg
    16681 ccccgg
//
LOCUS       DASRJQ010000235         3159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1186230, whole genome shotgun sequence.
ACCESSION   DASRJQ010000235 DASRJQ010000000
VERSION     DASRJQ010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186230"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /locus_tag="VFB89_05350"
     CDS             <1..1393
                     /locus_tag="VFB89_05350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZM26759.1"
                     /translation="LAFGTLLVGCAVGPTYHRPPVDVAPQLGRAVDSVVVREAPRTAF
                     WDETGDTTLTRLIREGLAAGYDVQVAQARVRGARAARLLSALDLAPTITVSGSYTRQR
                     LAAATFPGFGSSFPDQNLWDAGFDAFWEIDVFGGLRRNLQGQSALVASAQEDLRDVRV
                     SLAAELARSYFELRGAQGQLAVAQRNAENQRRTLQVVEDRLGAGRGTAFDAERARAQL
                     GLTQAAIPSIESQIAGAQYRIGVLVARSPEQVATELAAARDLPSLPATIPVTSPDSLI
                     RQRPDVVSAERRLAAQTAFVGAAQADYLPRLSIQGGAGITSGDLNTFGGTGTTRYAVG
                     PVISWPALNLGRVKARVDASRAQEAEAKAQYQQTVLRAVEEAEASLVAYAKARTRLAR
                     LQDAASASERAADYARLRFEGGIADFLDVLDAERTMLDAQNQLASGRTDATTAYVAVY
                     KALGGTWPLGEAE"
     gene            1450..2031
                     /locus_tag="VFB89_05355"
     CDS             1450..2031
                     /locus_tag="VFB89_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZM26760.1"
                     /translation="MARPKSFDQDAVLDQAVQLFWERGYEGTSLADLEAHLGLGRQSL
                     YNTFGDKQSLFLKALERYRSQVSEQRMAELNAPGAGLDAIRAFLTASVESLTAPGQRR
                     ACLVANTILERGSEDPDALLRCNSARNGLERAFRRALAQAKAAGELPEALDVEATATL
                     LVAQTYGLSVLAKSGASAAELNAAVEALFAGLH"
     gene            2082..2840
                     /locus_tag="VFB89_05360"
     CDS             2082..2840
                     /locus_tag="VFB89_05360"
                     /inference="COORDINATES: protein motif:HMM:NF006118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZM26761.1"
                     /translation="MPAGIRIKGRVALVTGANRGIGRGLVEELLARGASKVYAAARRP
                     EAVAELAAAGGGRVVPLRLDITNAADVKKAAVQATDVDLLINNAGVLSHGFGEFEDPA
                     WLDAAPREFETNVLGTLRVSQAFAPVLARNGGGTLVNVSSVAGVVGMPPVLTYSTSKA
                     ALHSLTQSARQMLRPQGTFVAGVYPGPVDTDMAAQLTLPKVTAASVAEAILDGLEQGL
                     EEIYPDPFARDIGEVYTVNPKGLEERMAAASTAD"
     gene            2860..3111
                     /locus_tag="VFB89_05365"
     CDS             2860..3111
                     /locus_tag="VFB89_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26762.1"
                     /translation="MKDQRVSIVAGARTPFVKAGHAFATLGPLALAKHAVRGQGWAPI
                     DLLVSCNDSPPLGVAAGDYLAYPHFLSPSRPAFSWSRSG"
BASE COUNT          523 a          998 c         1102 g          536 t
ORIGIN      
        1 cttggccttt ggcacgctgc tggtcgggtg cgcggtgggc ccgacgtacc atcggccgcc
       61 cgtggatgtc gccccgcagc ttggtcgcgc tgtggactcg gtcgttgtgc gcgaagcgcc
      121 gcgcactgcg ttctgggatg agactggtga taccaccctg acccggttga tcagggaggg
      181 gctcgcggcg ggctacgacg tacaagtagc gcaggcgcgc gtgcggggcg ctcgggcggc
      241 acggctcctc tcggccctcg atcttgctcc gaccatcaca gtgagcggga gctacaccag
      301 gcagcggctc gccgccgcaa ctttccccgg cttcggctcc agctttccgg atcagaacct
      361 ttgggacgcc gggttcgatg ccttctggga gatcgacgtc tttgggggtc tgcggcgaaa
      421 tcttcagggt cagagcgccc tggtcgcgtc ggcccaggaa gacctgcgtg acgtccgggt
      481 ctcgttggcg gccgagctcg cgcgctccta cttcgagctt cgcggagcgc agggtcaatt
      541 agcagtcgct cagcgcaatg ccgagaatca gcggcgcacg ctgcaagtcg tggaagaccg
      601 gctcggggcg ggtcgcggca cggccttcga tgcggagcgg gcaagggccc agttgggtct
      661 cacgcaggcg gcgattccct ctatcgagtc gcagatcgcg ggagcgcagt atcggatcgg
      721 cgtgctggtg gcgcggtcac ctgaacaggt ggcgacggag ctagccgctg cccgcgacct
      781 gccttcgttg cccgcgacga tcccggtaac cagcccagac tcgctcatcc gccagcgtcc
      841 cgacgtagtc agcgcagagc ggcggttggc ggcgcaaacg gccttcgtcg gtgcagcaca
      901 ggccgattat ctgcctcgac tcagcataca gggcggcgcg ggcatcacat cgggagacct
      961 gaacacgttc ggcggaaccg ggacgacacg ctacgccgtc ggaccggtga tctcatggcc
     1021 cgcactcaac cttgggcggg tgaaggcacg ggtggatgcg tcgcgtgcgc aggaggcaga
     1081 ggccaaagcg cagtaccagc agaccgtgct gcgcgcagtg gaggaagccg aggcgtcact
     1141 cgtggcatac gcaaaggccc ggacccgtct cgcgcgactg caagacgccg cctcggccag
     1201 cgaacgcgcg gccgactacg cccggctccg gttcgaaggg ggcatcgcgg acttccttga
     1261 cgtgctcgat gcggagcgca ccatgctcga cgcccagaac cagctggcga gcggccgcac
     1321 cgatgcgacg acggcgtacg tggccgttta caaggcgctg ggcggaacgt ggccgctggg
     1381 cgaggctgag tgatcgagcg gcgactccgg gtacttgact gatcagtcaa cataggctac
     1441 tctggacgca tggcgcgccc caagtccttc gaccaagacg ccgtactaga tcaggccgtg
     1501 cagctgttct gggagcgcgg ttacgagggc acctcgttgg ccgatctgga ggcgcacctt
     1561 gggctcggtc gccagagcct ctacaacacc ttcggcgaca agcagagtct tttcctcaag
     1621 gcgctcgagc ggtatcgaag ccaagtcagc gagcagcgga tggcggagct gaacgcgcct
     1681 ggcgccggcc tcgatgcaat tcgcgcgttc cttacggcaa gcgtcgagtc gctcaccgcc
     1741 ccagggcaaa ggcgtgcctg cctggtcgcc aacactattc tcgagcgcgg gtcagaggac
     1801 cctgatgccc tcctgcgctg caacagcgcc cgcaacgggc tcgaacgtgc attccgtcgg
     1861 gcgctggctc aggccaaggc cgccggggaa ctgccggagg ctctggacgt ggaggccacg
     1921 gcgacgctcc tggtcgctca gacctacgga ttgagcgtcc tggcgaagag tggggcgagt
     1981 gcggccgagc tgaatgcagc cgtggaggcg ctgttcgcgg ggctacacta gtgtttttac
     2041 atccaatttt tgaccgatcg gtcacaaaca gggaggtcgc aatgccggca ggaatccgga
     2101 tcaaagggcg ggtggctctg gtcacgggag cgaatcgtgg gatcgggagg ggtctggtcg
     2161 aggagctcct cgcgcggggg gcgagcaaag tctacgctgc agctcgtagg cccgaggcag
     2221 tggccgagct cgcggccgcc ggcggagggc gggtcgtgcc ccttcggctc gacatcacga
     2281 acgcagccga cgtaaagaag gcggccgttc aggcgaccga cgtcgatctc ctgatcaaca
     2341 acgccggcgt gctcagccat ggcttcggcg agtttgagga ccccgcctgg ctcgatgccg
     2401 ctccccggga gttcgagacc aacgtgttgg ggacattgcg cgtttctcag gcattcgcgc
     2461 cggtgttggc gcggaatggc ggggggacgc tcgtcaacgt aagctcggtg gcgggggtgg
     2521 tcgggatgcc acctgtgctc acgtatagca catccaaggc cgctcttcac tcccttaccc
     2581 agagcgcccg gcaaatgctg cggccccagg gaacattcgt ggcgggcgtc tatccaggac
     2641 cagtagacac cgacatggcg gctcaactca cgctcccgaa ggtcaccgcg gccagtgtcg
     2701 ccgaggctat cctcgacggg ctggagcagg gtctcgaaga gatttatccc gacccgttcg
     2761 cgcgcgatat cggggaggtg tacacggtga atccgaaagg gctcgaggaa cgaatggccg
     2821 cggcaagtac ggcggactga ctgtgaggca ggagaggcca tgaaggatca gcgggtttcg
     2881 atcgtagcgg gcgcgcgcac gccgttcgtc aaggccggcc acgcgtttgc gacgctcggc
     2941 ccgctggcgc tggcgaagca cgccgtccga ggccagggat gggcacccat cgatttgctc
     3001 gtctcgtgca atgacagccc gccccttggc gtcgcggccg gtgactacct tgcgtacccg
     3061 catttcctgt ctccttctcg tcctgctttt tcatggtcac gttccggctg accaggtggc
     3121 cggtctgtcc ccgattggcg gtgcgagccg tccttgcct
//
LOCUS       DASRJQ010000236         5565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2879504, whole genome shotgun sequence.
ACCESSION   DASRJQ010000236 DASRJQ010000000
VERSION     DASRJQ010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5565
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2879504"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1777)
                     /locus_tag="VFB89_05370"
     CDS             complement(<1..1777)
                     /locus_tag="VFB89_05370"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HZM26763.1"
                     /translation="MLIHRLRLHNFRQHEHTDLTLGPGLTGVIGPNGAGKTTLLEAVA
                     WAMYGMPAARGGKDSIRRRGTPARAQVEVELEFSLGAHHYRIVRGLTQAALYQDRDPA
                     PIANSLGTVTDRIGRLLGMSRDEFFNTYFTSQKQLAVMAAMGPSDRAQFLSRVIGYDR
                     IRIAQDRLRERRSALRARVDTLRGSLPDPEQLDREEAAAAARLKAARSGEKHASANRV
                     AAAAKLEKVRPEWERLQQLRDRALAAESELRVAEHELSAAKERAERLERQAADVATAQ
                     RRLDELFEQLEPLIGLQEEAEQFARKAQKFAARHRVQAQLAEVRQTLEAATAHLARLP
                     DVAALDKARARLEQTEAALAVAAADAETQRTAWVRDAQDASTKREALLAQFKDLKEQR
                     DRIVQAGPDGDCPTCARPLGAEYSKVLGMLDRQVEEVVSNGNFYRQRLEQLKIEPPDL
                     VTANQRKEELEQSLQSGSAEVGRLATQVQERSVVAGEETRLADRVTALRAELAQLPSA
                     EDEQRYAEVQRRIKALEPFVLEATRFKAMADQGPAVKAELTAIKATQDSAGARVIALQ
                     AELSALKYDERAFRKARDAAQAAETA"
     gene            complement(1777..2955)
                     /locus_tag="VFB89_05375"
     CDS             complement(1777..2955)
                     /locus_tag="VFB89_05375"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HZM26764.1"
                     /translation="MKLAHIADPHLGARQYHRLTPTGINQREADVAQAFRAAVDGVIS
                     ARPDVVVVAGDLFHTVRPTNAAIVFAFRQLQRLREALPEAPVVLVAGNHDTPRSSETG
                     SILRLYAELGVSVATDEARRFVFRELDLAVLAVPHQALAAPERPVFRPEGDEHRQVLV
                     LHGEVEGVFPFDRTSVEYGGATVNRRELHLDEWTYVALGHYHVQHEVAPNAWYSGSLD
                     YVTPNVWGELADDLAHGLGGKGWLLADLDSGKVERRPVPLARAVLDLEPIAADGLSAA
                     DIDSRIAERVANVSGGVRDRIVRLRVLDVPRYVARELDHAAIRTLKAEALHFHLDVRR
                     PESERTLGVGSPGRRQTLPELVATYLARWPLPGDVDRDTFVQRGVDLMDAVERDLTAG
                     "
     gene            3100..3627
                     /locus_tag="VFB89_05380"
     CDS             3100..3627
                     /locus_tag="VFB89_05380"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZM26765.1"
                     /translation="MGTHAGGRPAAARTAQVRAALDALRRIVQGLRMFSTRAEQATGL
                     SGAQLFVLQQLAEAPARSLNELAVRTRTHQSSVSTVVTRLVSRGLVSRRRAAADNRRL
                     LLQLTPAGRALLAKSPETAQSRIIAALEKMPERRRVAQVDALESLVETLGMAGAAPTL
                     LLEPVTDSRWKRQRK"
     gene            3606..4754
                     /locus_tag="VFB89_05385"
     CDS             3606..4754
                     /locus_tag="VFB89_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HZM26766.1"
                     /translation="MEATTKMMTRRSLLAAGVAGLGGLIACRVGRSDIPASLPEPPEG
                     GLVIRNARPLDAESPLSQLRSFLTPNEHFFVRSHFGPPASFPAEWTLTVDGSVARPVT
                     FTLADLRKLGSTTQVVTTECAGNGRGRLRLPRTAGVQWQYGAISTAEWTGVPLRGLLE
                     RAGMTAEAQHLWMEGLDQAPLPAVPKFLRSIPADLGLSHGFLAWEMNGSPIPLLHGGP
                     LRLLTPGWFGMASTKWLTHVHVRPAASDNFFMATAYRLADGSPVERLRVKSLITAPLA
                     DERLSVGTVQVQGQAWSGLGAGGIRSVELSLDDGQTWQPARLTGKELPYAWRSFESEV
                     RITSPGPQRLLARATDRSGAVQPRNAEANPGGFANNSMHELRFDAVQA"
     gene            4738..5124
                     /locus_tag="VFB89_05390"
     CDS             4738..5124
                     /locus_tag="VFB89_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26767.1"
                     /translation="MRFRPEAIFVCWIGLAASASGCRPASGQAAGNAPTQAAPPGPSA
                     ALSEVIAQSEKTSREALPPGSGRALVESNCLICHGAALIQQQHKDSTGWTKTVKLMRG
                     WGSPLTDADESVLVDYLIKSYGPEGR"
     gene            complement(5128..>5565)
                     /locus_tag="VFB89_05395"
     CDS             complement(5128..>5565)
                     /locus_tag="VFB89_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26768.1"
                     /translation="AAGLPVFAARWAPIPLLGRADQRLSLLAMGSLVGSPLLLLFMPG
                     GELNAGRLLGLNLAYLLLAAGGRLPSDQGSMGRRALRILLAVALAGMTLTFVPASTPL
                     SGWEWGGGLGELLAGAIPPFVMLIGTVLLGRRLGLYPAVSPKT"
BASE COUNT          927 a         1918 c         1827 g          893 t
ORIGIN      
        1 ccgccgtctc cgccgcctgg gccgcgtccc gggctttgcg gaaggcccgt tcatcatact
       61 tcagcgccga cagctccgct tggagcgcaa tcacccgtgc gccggcactg tcctgagtcg
      121 ccttgatggc ggtcagttcc gccttcaccg cgggaccctg gtcagccatt gccttgaacc
      181 gggtcgcctc cagcacgaag ggctcgagcg ccttgatccg gcgttggacc tccgcgtagc
      241 gctgctcatc ctcggcacta ggcagttgcg ccagttcagc ccggagcgcc gtcactcggt
      301 cggcgagccg cgtctcttcc ccggcaacga ccgagcgctc ctgaacctgg gtcgcaagcc
      361 gtcccacctc ggccgacccg gactgaagcg attgctccag ctcttccttg cgctgattcg
      421 cagtgaccag gtccggcggc tcgattttga gctgctccag gcgctggcga tagaagttgc
      481 cgttggacac gacctcctcc acctgccgat cgagcatgcc gagcactttg gagtattcag
      541 cgccgagggg gcgggcgcag gtggggcaat cgccgtccgg accggcttgc acgatccggt
      601 cacgctgctc cttcaaatcc ttgaactgtg ccagcagagc ctcgcgcttc gtgctggcgt
      661 cctgcgcatc gcgaacccag gcagtccgct gagtttcggc gtccgccgcc gcgaccgcca
      721 gggcggcctc cgtttgctcg agccgagctc gcgccttgtc cagcgctgcg acatccggaa
      781 gcctggccag gtgcgccgtc gcagcctcaa gagtttgtct cacttcggcg agctgcgcct
      841 gcacccggtg ccgggccgcg aacttctgag ccttgcgggc aaactgctcg gcctcctcct
      901 gcaggccaat cagcggctcg agctgctcga acagctcgtc gagccgccgc tgagcggttg
      961 caacgtcggc cgcctgacgc tccagccgct cggcgcgctc cttcgccgcg ctcagctcat
     1021 gctcggcaac ccgtagctcg ctctccgcgg ccagggcccg atcgcggagc tgctgaagcc
     1081 gctcccattc cggccgcacc ttctccagct tcgctgctgc agccacgcgg ttcgccgagg
     1141 cgtgtttctc gccggatcgc gccgccttga ggcgagctgc cgccgccgcc tcttcccgat
     1201 cgagctgctc ggggtccgga agacttcccc gcagcgtatc gactcgtgcc cggagcgcgg
     1261 agcgtcgctc cctcaaccga tcctgcgcga tccgaatgcg gtcgtatccg atcacccggg
     1321 acaggaactg agcccggtcc gacggcccca tggccgccat gaccgcgagc tgcttctgac
     1381 tggtgaagta ggtattgaaa aactcgtcgc gagacatgcc cagcaggcga ccgatgcggt
     1441 cggtcaccgt gcccaggctg ttggcgatcg gcgccgggtc tcggtcctga tagagcgcgg
     1501 cctgggttag tccgcggacg atgcggtagt ggtgtgctcc cagggaaaac tcgagctcga
     1561 cctcgacctg ggcccgggcc ggcgtccccc gccggcgaat cgagtccttg ccgcctcgag
     1621 cagccggcat cccgtacatc gcccacgcga cggcctcgag cagcgtcgtc ttccccgctc
     1681 cattcgggcc aatgaccccg gtgaggcccg ggccgagcgt gagatcggtg tgctcgtgct
     1741 ggcggaagtt gtggaggcga aggcgatgaa tcagcatcag ccggccgtca ggtcacgctc
     1801 gaccgcgtcc atcagatcga cgcctctttg gacgaacgtg tcgcgatcca cgtcgccggg
     1861 gagcggccag cgggcgagat aggtagccac cagctcggga agcgtctggc gccggcccgg
     1921 cgatcccacg ccgagcgtgc gctccgactc gggccgtcga acgtcgagat gaaagtgcag
     1981 cgcctcggcc ttaagggtcc gaatcgcagc gtggtccagc tcccgggcca catagcgggg
     2041 cacatccagt acgcgaagtc gcactattcg atcacgcacg ccaccggaaa cgttcgccac
     2101 gcgttcggcg atccggctat cgatgtcggc cgcactcagt ccgtccgccg ctatcggctc
     2161 gaggtcgagc acggcccgcg ctagcggcac gggccgacgc tcgaccttgc ctgaatccag
     2221 gtcggcaagt agccagccct ttccgccgag gccgtgggca agatcgtccg cgagctcacc
     2281 ccagacgttg ggggtgacgt agtccagcga tcccgagtac cacgcattcg gtgcgacttc
     2341 atgctggacg tgatagtgcc ccagcgcgac gtaggtccac tcgtcgagat gcagctcgcg
     2401 gcggttgacg gtggcgccgc cgtactccac gctggtccga tcgaagggga acacgccctc
     2461 gacctcaccg tgcaggacca acacctgtcg gtgctcgtca ccttccggcc ggaataccgg
     2521 ccgctcggga gcggctagcg cctggtgcgg tactgcaagc accgcgaggt cgagctcgcg
     2581 aaatacgaag cggcgtgcct cgtccgtcgc taccgagacg cccagctcgg catagaggcg
     2641 gaggatagag ccggtctcgg acgagcgagg cgtatcgtgg ttgccagcga ccagaaccac
     2701 cggagcctcg ggcagcgcct cgcgcagccg ctgcaactgg cggaaggcga agacgatggc
     2761 tgcgttggtg gggcggactg tgtgaaacag gtctccggcc acgaccacca cgtcagggcg
     2821 cgccgagatg acgccgtcga cggccgctcg gaaggcctgg gcaacatcgg cttcacgctg
     2881 gttgatgccg gtgggagtca ggcgatgata ttgccgtgcc ccgaggtggg gatcggcgat
     2941 gtgggcgagc ttcacggggt ggagaatgca ggagcgtgcc ccttcgggca agtcgcaaac
     3001 cgacgacacg ccgtcagacg cgtcaggcga catcttgccg agttctcgga gcgccttgcg
     3061 gcttgtccgc ccccgctgcg ccgcgtatcc tgctctttca tgggaaccca tgcaggaggt
     3121 cggccggcag ccgcccgcac ggcgcaggtt cgggcggcgc tcgacgcgct ccgccgcatc
     3181 gtgcaaggtc tccgtatgtt cagcacccga gccgagcaag ccaccggctt gagcggggca
     3241 cagctcttcg tgctccagca gctggccgaa gctcccgccc gatcgctgaa tgagctcgcc
     3301 gtccgaaccc ggacccatca gagctcggtg tctacagtgg taaccaggct ggtctcacgt
     3361 ggcctggtat cccgccgtcg agcggcggcc gacaaccggc gcttgctgct gcagctgacg
     3421 ccggccgggc gggccctcct ggcgaagtcg ccggagacgg cgcaaagtcg tatcatcgcg
     3481 gccctggaga aaatgcccga gcggcgcagg gtagcccaag tcgacgcgct ggaaagcctg
     3541 gtggaaaccc tgggtatggc gggcgcggcg ccgaccctgc tgctcgagcc cgtcaccgac
     3601 tctcgatgga agcgacaacg aaaatgatga cccggaggag cctcctcgca gcaggcgtag
     3661 caggcctggg cggcctgatc gcgtgccggg tgggtcgctc cgacatcccg gcaagcttgc
     3721 cggaaccgcc tgaaggtggg ttggtgatcc gcaacgctcg tccactggat gccgagtccc
     3781 cgctcagcca actgcgctca tttctcacgc ccaacgagca cttcttcgtc cgcagtcact
     3841 tcggcccgcc cgcatcgttc ccagccgaat ggacgctgac cgtcgacgga tccgtcgccc
     3901 gacccgtgac ctttacactg gcggacctcc gaaagctcgg gagtacaact caggtcgtta
     3961 ccaccgagtg tgcgggcaac ggcaggggca ggctccgatt gccccggacc gcgggtgtgc
     4021 agtggcagta cggcgcgata agtacggcgg aatggaccgg cgtaccgctg cgtggtctgc
     4081 tcgagcgcgc cggaatgacc gccgaggcgc agcacctctg gatggagggc ctggaccagg
     4141 ctccgctccc tgcagtaccc aagttcctgc gcagtatccc ggccgatctg gggcttagcc
     4201 acgggttcct cgcgtgggaa atgaacggct ccccgatccc gttgttgcac ggtggtccac
     4261 tgcgcctcct cacaccgggc tggttcggga tggcgtccac caaatggctg acccatgtgc
     4321 acgtccggcc ggcggcatcg gacaacttct tcatggcgac ggcataccgc cttgccgacg
     4381 gaagtccggt agagcgtctt cgggtcaagt cgctgattac ggcaccgctc gccgacgagc
     4441 ggctcagcgt cggcacggtg caggtacagg ggcaggcctg gtccgggctg ggtgcgggcg
     4501 ggatcagaag cgtggagctg tcgctcgacg acggtcaaac gtggcaacct gcccggctca
     4561 cgggtaagga gctaccgtac gcctggcgca gcttcgaatc cgaggtccgc ataacctcgc
     4621 ccggaccgca gcggctgctt gcgcgggcaa cggatcgctc aggcgccgtc caaccgcgga
     4681 atgccgaggc gaatccaggc ggattcgcca acaactccat gcacgagctt cgattcgatg
     4741 cggttcaggc ctgaagcgat tttcgtttgc tggatcggtc tggccgcgag cgcgagcgga
     4801 tgccgaccgg cctcaggcca ggcagccggc aacgcgccga cgcaagcggc accacccggt
     4861 ccatcggcag ctctctcgga ggttatcgcg cagagcgaaa agacatcacg cgaagcgctg
     4921 ccgccaggga gcggtcgagc cctggtggag agcaactgtc tcatctgcca cggcgccgcc
     4981 ctgattcagc agcagcacaa ggactcgacc ggatggacga agacagtgaa gctgatgcgt
     5041 ggctggggct cgccgctaac cgacgcagat gagtccgtgc ttgtcgatta tctgatcaag
     5101 agctacggcc cggaggggcg ctgacgctca ggtttttggc gacacagccg gatagaggcc
     5161 cagccgccga cccaacaaga cggttcctat cagcatgacg aacgggggaa tcgccccggc
     5221 cagcagctcg cccaaacctc cgccccattc ccaacccgac agaggcgtcg acgccggcac
     5281 gaacgtgagc gtcatgccgg ccagtgccac cgcgagcaaa atgcgaagtg cacgccgccc
     5341 catggagcct tgatccgatg gcagccgacc gccggcagcg agcagcagat atgccagatt
     5401 gagtcccagg agccgccccg cgttcagttc gccgcccggc atgaacagca gcagcaacgg
     5461 cgacccgacc aggcttccca tggccagcaa gctgagccgt tggtcggcac gaccaagcag
     5521 tgggatcggc gcccagcgag cggcaaagac cgggagtccg gccgc
//
LOCUS       DASRJQ010000237         2267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3851320, whole genome shotgun sequence.
ACCESSION   DASRJQ010000237 DASRJQ010000000
VERSION     DASRJQ010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3851320"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1158
                     /locus_tag="VFB89_05400"
     CDS             157..1158
                     /locus_tag="VFB89_05400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HZM26769.1"
                     /translation="MAEETKERQLNGSANPSGRFSRERAAEGPTDLTRRSWGGVLKRS
                     VREFKEDNLSDWAAALTYYGILSIFPALLVLVSVLGLIGTSATQPLIDNLGKVAPGPA
                     QEIATSALKNLQGSQGAAGVIFIIGLVGALWSASGYVAAFMRASNRIFDIDEGRPVWK
                     TVPTRVVLTLVLVLLLALSAVAVTLTGGVAKEVGGVFGLSDTAITVWNIAKWPVLLVV
                     VSLMFALLYWAAPNVKHPGFRWVTPGGVLAVIGWVIASLAFALYVANFGSYNKTYGAL
                     AGPIVFLVWLWISNIMVLLGAEVNAELQRGRAIEAGHPEHREPFVEPRDTRKMSDAG"
     gene            complement(1162..1512)
                     /locus_tag="VFB89_05405"
     CDS             complement(1162..1512)
                     /locus_tag="VFB89_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26770.1"
                     /translation="MELADRLRWALEARRPVAAAWDPERLRAEPAFGLLRAAEFELLR
                     TGEFELLRVEPELELRRLDLAAVAPLSLAAGRLRLPAFGGALCFGLVCLATSPHSTAE
                     HQEEHVDATSIAAG"
     gene            1688..1864
                     /locus_tag="VFB89_05410"
     CDS             1688..1864
                     /locus_tag="VFB89_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26771.1"
                     /translation="MKIPRGVVPRNFKLSVPRGLDVKAIATQVEQAADRLERTSEDVR
                     LASAQAKRISQKLS"
     gene            complement(1883..1998)
                     /locus_tag="VFB89_05415"
     tRNA            complement(1883..1998)
                     /locus_tag="VFB89_05415"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1920..1922),aa:Val,seq:cac)
BASE COUNT          388 a          712 c          793 g          374 t
ORIGIN      
        1 tccgcccgcg agcccggtcg acgcgccggt gccggctgca gctaggcccc ccttacggcg
       61 cttaccgcaa actgaacgcg atttcccaga aggggtaact ggcgaagggt cggggtacgg
      121 catgaggagg acggcccact gacacggagg ttgggaatgg ccgaggagac caaggaacgg
      181 caactcaatg gaagcgcgaa cccttccgga cgtttctccc gcgagcgcgc agccgaaggc
      241 ccaacggacc tgacgcgccg ttcgtggggt ggcgtcctga agcgcagtgt cagggagttc
      301 aaggaggaca acctgagcga ctgggcggcg gcgctcacgt attacggcat cctttcgatc
      361 ttcccggcgc tgctcgtgct ggtctcggtc ctggggctga tcggcacctc ggcgacgcag
      421 ccgctgatcg acaacctcgg aaaggtcgca cccggtcccg cgcaggagat tgcaacgagc
      481 gccctcaaga acctgcaggg ctcccagggc gcggcgggag tgatcttcat catcgggctc
      541 gtcggtgcac tctggtcggc ctccggctac gtcgcggcct tcatgcgcgc gtccaacagg
      601 atcttcgaca tcgacgaggg ccgacccgtc tggaagaccg tgccgacgcg ggtggtcctg
      661 acgctcgtcc tggtgcttct gctggcgctg agcgcggtcg cggtcacgct taccggcggt
      721 gtcgccaagg aggttggcgg agtattcggt ctaagcgata cggcgataac ggtatggaac
      781 atcgccaagt ggcctgtgct gctggtcgtg gtcagcctca tgttcgcgct gctctactgg
      841 gcggcgccca acgtgaagca cccgggattc cgctgggtta cgcccggtgg cgtgctcgcc
      901 gtgatcggct gggtgatcgc ctcgctggcg tttgccctgt acgtcgcgaa cttcggctcg
      961 tacaacaaga cctacggtgc actcgcgggg ccgatcgtgt tcttggtctg gctgtggatc
     1021 tcgaacatca tggttctgtt gggggccgag gtgaacgccg agctacagcg cgggcgggcg
     1081 atcgaggcgg gtcaccccga acacagagag ccgttcgtcg agccacgcga cacccgcaag
     1141 atgagcgacg caggctgagg tctagcccgc cgcaatcgat gttgcgtcga cgtgttcttc
     1201 ctgatgctct gccgtagagt ggggcgaggt ggccagacag accaatccga agcaaagcgc
     1261 cccgccgaag gcaggcaagc gcaatcgccc tgcggcgagc gacagcggcg cgaccgctgc
     1321 caagtcgagc cgacgtagct cgagctcggg ctcgacgcgc aagagctcga actcccccgt
     1381 gcgcaagagc tcgaactccg ccgcacgcaa gagcccgaac gccggctccg cacgcaagcg
     1441 ttcgggatcc caagcggctg cgaccgggcg ccgggcttcg agcgcccagc gaagtcggtc
     1501 tgcgagctcc aagggcgccc ggcaacgaaa tgtcgatgct ggcgcggttg ggcaggtcgc
     1561 ggccaaggcg ggcagcaatg cgggcaggat cgcgaagggc gcgctagggg tgatcgtggg
     1621 tggtgcggca gtgggcctcg caggacgcgc agccgtaaaa cgcaagcggc gccctacggt
     1681 gctcggggtg aagatcccgc gtggggtcgt tccccgcaac ttcaaactga gcgtgccgcg
     1741 cggcctcgat gtcaaggcca tcgcgacgca ggtcgagcaa gccgctgacc gcttggagcg
     1801 cacgagcgag gacgtgcggc tcgccagcgc tcaggcgaag cggatcagtc agaaactttc
     1861 gtaggacgag tggctctcgt tatgggcgcg gctgggttcg aaccagcgac ctctcgcgtg
     1921 tgagacaagg gacctctgcg gcatggatgg ccgcttttcg ctgcaaatct cgctatttca
     1981 tgcagatcta cagcgcccga agtagggcta gatcgggccc tgtagggcct ggtttgagac
     2041 aaggattgag acaacggcgg tcggacgcag cggtctcgct ccggagaggg gcgatggctg
     2101 ccatgtcgcc atcacgcgag ggcggcaccc gctcctcgtc cgactcacta gcgggtccgt
     2161 ctgcccggcg ctctcgcttc tgcctcattg cgatagcccg tcgcgccagg tgtcgccgcg
     2221 acgagctcgg ctcacgctgg cgtcgtcgaa cgcagaccgg gcagtgt
//
LOCUS       DASRJQ010000238         4974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2025296, whole genome shotgun sequence.
ACCESSION   DASRJQ010000238 DASRJQ010000000
VERSION     DASRJQ010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2025296"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VFB89_05420"
     CDS             <1..527
                     /locus_tag="VFB89_05420"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZM26772.1"
                     /translation="IALRFAADKHRDHRRKDPDASPYINHPIAVAEVLARIGGVTDLA
                     VLQAAVLHDTIEDTATTEAEIEQAFGVEVSRLVAEVTDDKTLPKAARKRLQVQHAPHL
                     SRCAKLIKLGDKICNIQDVTHSPPENWSLERRLEYLDWSRQVVAGCRGVSSELERHFD
                     LLALEADAAMRSVR"
     gene            765..1229
                     /locus_tag="VFB89_05425"
     CDS             765..1229
                     /locus_tag="VFB89_05425"
                     /inference="COORDINATES: protein motif:HMM:NF020177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1772 domain-containing protein"
                     /protein_id="HZM26773.1"
                     /translation="MVRSVQFFALLLTALSMGVHFGTWLTEGPLRDTQSGALFVEIHK
                     GRDRVVARVMPILGSASLIFLALGVFLVRAVPAAFVLSLAGLLLCMGDMLVTVLVNVP
                     INGKVQSWQPNAPPPEWTLLRDRWERFHSIRSLLVVSGFALFAASVVFFDVS"
     gene            1444..1947
                     /locus_tag="VFB89_05430"
     CDS             1444..1947
                     /locus_tag="VFB89_05430"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HZM26774.1"
                     /translation="MTRGIAILAAATLLAFGLACEPHGHRPGLWLRGESIHTAIADWS
                     FSDAFPEITVETRTWYLLPHSVTTASAVSEGHLYVPSVYGSSAKFPEARFWHRNIARD
                     PRVRVKVGDRVYDRRAVLVNDPAEQEAALAAFARKSPFWRDLLAKPDSERGTIVLLRM
                     DDPAPES"
     gene            complement(2160..2732)
                     /locus_tag="VFB89_05435"
     CDS             complement(2160..2732)
                     /locus_tag="VFB89_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26775.1"
                     /translation="MSTLVMVKRGREMCMAAETLASFGSRKQSARYVARPEKIIRLGS
                     SYVGTVGWSVTRTVLESALSHGSELPEVRSELELFEFSRAFHQRLKDEYFLNAAADPK
                     DPYESSQMTLFVMNRCGLFAMYSLRTVEQCERFTAAGSGADYALGAMHAGYELGLPVE
                     DVARIGVEAGIEFDDGSLGPITLKTMKLEA"
     gene            3122..3586
                     /locus_tag="VFB89_05440"
     CDS             3122..3586
                     /locus_tag="VFB89_05440"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HZM26776.1"
                     /translation="MGDAEHEILIREALSLAYAAREKGDHPFGAVLAIGNRVLLSAEN
                     SVVSDGDPSRHAELNLIQLAWARLAKAEIESATLYASTEPCPMCTGAIYYSRVPRVVY
                     SVSQTKLSELTGGRFALPCIALLGRPERRTEVIGPVLPEEGYRVHVGFWPSP"
     gene            4049..4297
                     /locus_tag="VFB89_05445"
     CDS             4049..4297
                     /locus_tag="VFB89_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26777.1"
                     /translation="MSRSGWIPASLLDPGQRLAARLLLRGFTRRSPVVCSPPNKALQL
                     TGRRLPACQGSQPPAARVRVDSPGGRRSWHPGSRAAGS"
     gene            4344..>4974
                     /locus_tag="VFB89_05450"
     CDS             4344..>4974
                     /locus_tag="VFB89_05450"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZM26778.1"
                     /translation="MSDRWAAGATYEDFMGRWSRLLAPRFVSWLSIRPNANWLDVGCG
                     TGALAEAICAGANPASVVACDPSEPFIEYARRCGVGTPISFIVAGVGQLPMHPGGFDS
                     VTSLLALNFFPKPEVAIKEMRRITAVNGVVSACVWDYAGRMEFLRCFWDSAAAVDPTA
                     AELDEGRRFPICQPDALEPLFCEAGLADVVSDSIEISTRFPTFAEFWKPF"
BASE COUNT          803 a         1687 c         1523 g          961 t
ORIGIN      
        1 tcatcgccct tcgctttgcg gcggataagc atcgcgatca tcgacgcaag gacccggatg
       61 cttctcccta catcaaccat cccatcgctg tcgcggaggt cctcgctcgc atcgggggag
      121 tcacggatct ggcggtcctc caagccgccg ttctccacga cacaattgag gatacagcca
      181 cgaccgaggc agaaatcgag caggccttcg gggtcgaagt ctcgcgcttg gtcgcggagg
      241 tcaccgatga caagacactc ccgaaggccg cgcgaaagag gctacaggtc cagcacgcgc
      301 cgcatctctc gcgttgcgca aagctcatca agctaggcga taagatctgt aacatccaag
      361 acgtcactca ctcgcctccc gagaactgga gccttgagcg gcgactcgag tatctcgact
      421 ggagccggca ggtggtagct ggttgccggg gtgtaagctc cgagctcgag cggcacttcg
      481 atctgcttgc actggaagct gatgcagcga tgcgttcagt caggtaggca gaacttccca
      541 cctacggagg caacattgca ggctgcctaa caaagcgctg cagctgacgc gccgccgcat
      601 cggggcctgc ccggtcccgg gccggcgggc ggccggttgg gccatggact cgtcggccgc
      661 ccgccggagt ggcatcttgc tccacgggcg gcgcgcagct gagcgccggt ccgttgggcg
      721 ggcggtatcg gaagcatggc atctgtcact actagagggt tgctatggtc cggagcgtcc
      781 agttctttgc tctccttctc accgctctca gcatgggcgt ccacttcggc acgtggctca
      841 cggaaggacc gttacgcgat actcaatcgg gtgccctgtt cgtcgaaatt cacaaaggga
      901 gagatcgagt tgtagcgcgg gtcatgccga tcctcgggag cgcttcactt atcttcctcg
      961 cgcttggagt gtttctagtg cgcgcagttc cagctgcctt cgtcctctct cttgccgggc
     1021 tcttactgtg catgggcgat atgctcgtca cggtcctcgt gaacgtcccc atcaatggga
     1081 aggtgcagag ctggcagcct aatgccccac ctccagaatg gacgctcctt cgtgatcgct
     1141 gggagaggtt ccacagcatc cggtcccttc tagtcgtctc aggcttcgct ctcttcgctg
     1201 caagcgttgt cttctttgac gtctcgtgaa agggcgctcg gtccgccgcc caacaaggcg
     1261 ctgcagctga cctgccgccg ctccgggccc tgccaggtcg cccagccgcc ggccgcgggc
     1321 ggggctcgat cggtgacccg ggaggactcg gccgcggccg tcggtcggtc cttggtacaa
     1381 ctcacggcgg ccggcagctg agcgccggcc cgttgggcag ctgcagtgac atcgagaagg
     1441 cgcatgacac gtggtatcgc aatactcgca gcggccacac tccttgcttt tgggctcgcc
     1501 tgcgaacctc acggtcaccg accagggctc tggctccgtg gcgagtccat tcacactgcg
     1561 atcgcagact ggtctttctc ggatgccttc ccggagatca ccgtcgagac ccgaacctgg
     1621 tatctgcttc cccattccgt caccacagcc tccgccgtga gcgaagggca cctttacgtc
     1681 ccttcggtct acggaagcag cgcaaagttc ccggaggcaa ggttctggca tcgcaacatc
     1741 gcgcgcgatc ccagagtccg cgtcaaggtc ggtgatcgtg tctacgaccg gcgcgcggtc
     1801 ctggtcaacg atcccgccga gcaggaggcg gccctcgcgg catttgcccg gaagtctccc
     1861 ttctggagag atctcctcgc caagcccgac tccgagcgtg gcacgatcgt gcttcttcgc
     1921 atggacgatc cggcacctga gtcgtagctc ccgtttattt cagttggacg ctcacgaatc
     1981 cataggcagc tgcccaacaa ggcgctgcag ctgaccggcc gccgccggcc ggccgcccgg
     2041 gcccggagtc gattcgccgg gcgtccgccg gtcgttcctt ggtacactca cgggcggccg
     2101 gcagctgagc gccagtctgt taggcggcgc cggtagactc gggtccaccg gcgccgctat
     2161 caggcctcga gcttcatcgt cttcagggtg atcgggccca gcgatccatc gtcgaactcg
     2221 atcccagcct cgaccccgat ccgggccacg tcctctaccg ggagccccag ctcatagccg
     2281 gcatgcatcg cgccaagcgc gtagtcagca cccgatcctg cagccgtgaa tcgctcgcac
     2341 tgctcgaccg ttcgaagtga gtacatggcg aacagcccgc accgattcat tacgaagagc
     2401 gtcatctgtg aggattcgta cggatccttg gggtcggcag ccgcattcag gaagtactcg
     2461 tccttcagcc tctggtggaa agcccgcgag aactcgaaaa gttcgagttc acttcgaacc
     2521 tccggaagct cgcttccatg gctgagcgca ctttccagca cggttcgcgt cacactccag
     2581 ccaaccgtac cgacatagga gcttcccagc cggatgatct tttccggccg agccacgtac
     2641 ctggccgact gcttcctaga tccaaagctc gccagcgtct ccgccgccat gcacatttct
     2701 cgaccccgct tgaccattac caacgtcgac atacacttct cccgctcacg acgggcgcgc
     2761 cccgctgtgt agtccggcac acgagccccg cgggcccgtt ctgctctcac tgggtagttg
     2821 cttggcgcgg tcgtttcagg ccggggcgag agccaccggg cagacagtcc gcgcgcgcca
     2881 actgttctcg cgctttcggt caaacatgac agtctccccg agtagggagc gtttacttag
     2941 atcattcgtt ccgcagtgtc aaccccttga gcgccgccta acaaggcgct gcagctgccg
     3001 agccacagct cgattcaatc gacctctggt agagtctggc atcgaacttc ggcgcttcga
     3061 gcgagccacc gaggcgctgg ggctcgcagc tgagcgccgg tccgctgggc ggcgggaaga
     3121 gatgggagac gccgagcacg agatcttgat tcgagaggct ctctcactcg cttacgcggc
     3181 gcgcgagaag ggagaccacc ccttcggggc ggttctcgcg atcggcaacc gcgtccttct
     3241 gtccgccgag aactcggtcg tcagcgatgg cgacccttct cgccacgccg agctcaacct
     3301 cattcaactc gcatgggccc gtctcgcgaa ggccgagatt gaatctgcaa cgctgtacgc
     3361 gtccaccgag ccgtgtccga tgtgtaccgg cgcgatctac tattcacgtg ttccgcgcgt
     3421 cgtctattca gtgagtcaaa ccaagctcag cgaactcacc ggtggccggt ttgcgcttcc
     3481 atgcatagct ctcctgggcc ggccggagcg ccgaaccgag gtaatcggtc cggtcctccc
     3541 cgaagagggc taccgggtgc acgtcgggtt ctggccgtct ccctagcgcg ccgccgcccc
     3601 ggggcctgcc tggccttcca gccggcgggc ggccgggtcg cgggtcgatt cgccggccgc
     3661 cctccggtcg tggcatagtg cacgggcggc gcgcagctga gcgccggtcc gttaggcggc
     3721 gagactcagc gcctcggagg agccaatcga atccgccaag aagctgccgt ccagagctct
     3781 cctgcgcgcc tcctggcatc actcgctacc tcgtcccaca cgagaaagcc ggggccctcc
     3841 accgcaagac ctgtatcgcc gcccctcctt gcaatgccgt agcctgcgcc acgcccttgg
     3901 ttgcgccggc tcgccttctc acgctgcttc atcgtgtgtg tcccctcttt gaacagcgca
     3961 tcagttagcc tccaatcgac gcggcttact gtgaaccagc tcacaccact cgcacaccgc
     4021 ttcagcgagc cactggctgg cgtgccgcat gagccgatct ggatggattc ccgcgtcgtt
     4081 gctcgatccg gggcagcgcc tggctgcgag gctgctgctg cgggggttta cccgcaggtc
     4141 accggtggtt tgctcgccgc ctaacaaggc gctgcagctg accggccgcc gcctcccggc
     4201 ctgccagggc tcccagccgc cggccgcccg ggtccgggtg gactcgccgg gcggccgtcg
     4261 gtcgtggcat cctggttcac gggcggccgg cagctgagcg ccggtccgtt gggcggcgcg
     4321 aagaacgtgg cgtcatgcct gagttgagcg acaggtgggc agctggtgcg acctacgagg
     4381 acttcatggg ccggtggagt cgtctcctcg ccccgcggtt cgtgtcgtgg ctgtccatcc
     4441 gtccgaatgc aaactggctg gacgtggggt gtgggacggg cgcacttgcc gaggcgatct
     4501 gtgccggagc gaacccagca tcggtagtcg catgtgatcc gtccgagccg ttcatcgagt
     4561 acgctcgtcg gtgcggagtc ggcacgccga tctcgttcat cgttgccggg gtcggacagc
     4621 ttcccatgca cccaggtggg ttcgatagcg tcacttctct gctcgccctg aacttctttc
     4681 ccaagcccga ggtcgccatc aaggagatgc ggcggatcac cgccgtgaac ggcgtcgtat
     4741 cggcatgtgt gtgggactac gcggggcgca tggagttcct gaggtgcttc tgggattccg
     4801 ccgcggccgt ggaccccact gccgccgagc tggacgaagg tcgccgcttc ccgatctgcc
     4861 aaccggatgc gctcgaacct ctattttgcg aagcgggtct tgccgacgtc gtctccgact
     4921 cgatcgaaat ttccacccga ttcccgacgt tcgccgagtt ctggaagcca ttcc
//
LOCUS       DASRJQ010000239         4721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3724316, whole genome shotgun sequence.
ACCESSION   DASRJQ010000239 DASRJQ010000000
VERSION     DASRJQ010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3724316"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1257
                     /locus_tag="VFB89_05455"
     CDS             <1..1257
                     /locus_tag="VFB89_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26779.1"
                     /translation="PDVVAAVLSDAWGIPVPVEVIWPELRDGQPPAPAHLQPWAAART
                     LDELGLFLRSDMLTRRAVLAASVSVATGKELIAPIARWLSVDTGVLPLPGGAGPGQIN
                     IEAVEAIEEATRHFMAADAAVGGAVVRRAAAGHLLYAVDLATEARYSEDIGNRLLIAI
                     ADLAGHVGWMSHDAAMEGPAQRYLIYGLQAAREAHGDEAQLRAVGILADMSAQMNELG
                     HPSSALRLTDLAFEQLPADGRRFNKIRSLLWSQRAARLSGMGTVHLSEVRSAVGLAFD
                     LYGQLNEEDESPAVARCFPYTTEAEIASSAAKTYRTLALHDPTFAADAERQALSALAL
                     RPPGFQRSLVFDQINLAQARFLGGEPEQACQDGAQAIEMAARVTASSRVNARLAGLMT
                     DSEPFKKLSGVREFRERLRTAGDDKQ"
     gene            1704..2279
                     /locus_tag="VFB89_05460"
     CDS             1704..2279
                     /locus_tag="VFB89_05460"
                     /inference="COORDINATES: protein motif:HMM:NF033447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxE family protein"
                     /protein_id="HZM26780.1"
                     /translation="MTTTTEVDLDLLLKRRLIVARYGEMDLGRWWNTTQLGADGAQVL
                     KRGFPRTHHFAQARSVFVVASHRCDERFDPLGDVAATSVTLWRLPAEIEEAFDARWEE
                     WLDNAEDWTDLFVRLQKPQDDLVNVLLAEDAVALVDVDAHARGARRVDGRRVCVSNAF
                     SRSDSDVAQLALGFAHGASGELVVPYAGLSA"
     gene            2276..3058
                     /locus_tag="VFB89_05465"
     CDS             2276..3058
                     /locus_tag="VFB89_05465"
                     /inference="COORDINATES: protein motif:HMM:NF020425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HZM26781.1"
                     /translation="MTERANVASSFTVVKGAMIDETYAVMAGWDFELSKRANLDQLRA
                     ENFIGAKSVTWLRDVAKVINRRFDPAGRDRPLVHLAKSGCDLAEWKPLLLWHMTRDEF
                     LVRDFLQNWLYAEYEDGAFQVRPADVLGYLAEINKRGGQTEHDWSEATTKRVASGLLR
                     IAADFGLLRGAGTAKEFASYHLPERSFLYILHALRDQRVQPAKIITASDWRMFLMAST
                     DVEQEIYRLHQFKKLDYQVAGSIVQLSLPEGSSAGYAQRMVA"
     gene            3055..3633
                     /locus_tag="VFB89_05470"
     CDS             3055..3633
                     /locus_tag="VFB89_05470"
                     /inference="COORDINATES: protein motif:HMM:NF020329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX protein BrxB domain-containing protein"
                     /protein_id="HZM26782.1"
                     /translation="MRTLEERLRGSLEPILEQIDPARAGLSTYSDMPCALFVYDPEEE
                     FRLRTQVAMLETRLSQKGKRIRRISLAECLDEAMRSQRPLDQWFDAERSTGVEVVVET
                     VHAVLWEYSPLVDHVAARMPEDPDPTRDVVLILRAGALFPMYRTSALLEQLKGHVHVP
                     TVLFYPGRLDGGSGLSFMGVLDPEHNYRLKVF"
     gene            3649..>4721
                     /gene="brxC"
                     /locus_tag="VFB89_05475"
     CDS             3649..>4721
                     /gene="brxC"
                     /locus_tag="VFB89_05475"
                     /inference="COORDINATES: protein motif:HMM:NF033441.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HZM26783.1"
                     /translation="MTIRTLFAGDIDRKIEEVIKVDQTNEQVIRDELNEYVVTDAIRK
                     YYLEILDAYQETPNKPHEGIAIWVSGFFGSGKSSFAKVLGLAIENRSIDGKGAAERFA
                     DRGGDNRLRVLLRTISERIPTHTVIFDVSTDRGIRSGNQSITEIMYRQFLHSLGYATD
                     LDLAELEITLEEKGELQKFAAEYAKLFGKNWDDEKGKVAFSASEASQVMHSLLPQTFP
                     MADSWVRAAKSRADITPGKLAERATELMKRRRPGYTLLFVIDEIGQFVARDVQKMLDL
                     QAVVQQLGVKGRGKHWIVVTSQEKLDDLVSGLEGTRIELARLRDRFPLQVHLEPSDIS
                     EVTSKRILTKNAAAETRLGKLFD"
BASE COUNT          865 a         1436 c         1565 g          855 t
ORIGIN      
        1 cccgacgtgg tggccgccgt cctcagcgac gcctggggca ttcccgtacc ggtcgaagtg
       61 atctggccgg agctgcgcga cggccagccc ccagccccgg cgcacctgca accgtgggcg
      121 gccgctcgta cgctggacga gctggggctg tttctgagga gtgacatgtt gacgaggcgg
      181 gcggtactgg cggcttcggt ctccgtggcc accggcaagg agctgatcgc tccgatcgcg
      241 cgctggctca gcgtagacac gggcgtgctg ccgctgccgg gaggtgccgg tccggggcag
      301 atcaacatcg aagcggtgga agcgatcgag gaggcgaccc gccacttcat ggctgctgac
      361 gccgctgtgg gtggggcggt ggttcgcagg gccgctgccg ggcatctctt gtatgccgtg
      421 gatctagcca ccgaggcccg ctacagcgag gacatcggca accggctgct gatcgccatc
      481 gctgatcttg ccgggcatgt cggctggatg agtcacgacg ccgccatgga gggcccggcg
      541 cagcgttatc tgatctacgg cctccaggcg gcgcgtgagg cccatgggga tgaagcccaa
      601 ctgcgcgccg taggcatcct ggccgacatg tcagcccaga tgaacgagct ggggcatccg
      661 agcagcgccc tacgcctcac cgatctggcg ttcgagcaat tgcctgccga cggacgccgc
      721 ttcaacaaga tccgctcgtt gctgtggagc caacgtgcag cccgcctgtc aggcatggga
      781 acggtgcacc tgtctgaagt tcgcagcgcc gtcggcttgg cgttcgacct gtacggccaa
      841 cttaacgagg aggacgagtc gcctgctgtg gcaaggtgct tcccgtacac caccgaggct
      901 gagatcgcca gtagcgcggc caagacctac cgcaccttag ccctgcatga tccgaccttt
      961 gccgctgacg ctgagcggca ggcgttgagc gcccttgccc tgcgcccgcc tgggtttcag
     1021 cgctcgctcg tatttgatca gatcaacttg gcgcaagccc gttttctagg cggcgagcct
     1081 gagcaggctt gccaggacgg ggcgcaggcg atcgagatgg cggcacgggt cacggcctct
     1141 agccgtgtca atgcccggct ggccgggctt atgactgact ctgagccatt caagaagctc
     1201 tcaggcgtgc gggagttccg ggagcggttg agaacagctg gggatgacaa gcagtaggta
     1261 ggcggcgccc cgtccttgac gactgtgcct gggcatagcg cagcggtggc ggggtggcat
     1321 ccgcgtgggt gtcggttcct ggccaaacaa cgtcgttaac cggcgttcgt caatgtcagc
     1381 tcaagctaag gattcagcct cgcttcacta atcatgtgtt ccacaggtaa cgaacgtttt
     1441 ctggatctgc aggtagtttt tccggacgaa attcctaaca tctaacaaaa gggtgagcgg
     1501 aagatccacg gccgcggtgc ccacttacaa accgtccatt cgagccgcga ccggaccgta
     1561 ggctgcgcga tcttgcccag aacgcgggtg cgagggcagg gccccgcgcg ggacgcgaca
     1621 cgaccgaggg gacggggttg ctgggctgtc gcgttgacta ggtagggtca tggcgatggt
     1681 gagcgccggc ccgaggacgc agcgtgacga cgacaaccga ggtcgatctt gacttgctgc
     1741 tgaagcgtcg actcatcgtg gcccggtacg gcgagatgga tcttggccgt tggtggaaca
     1801 ccacccagct cggcgctgat ggtgcacagg tgctcaagcg ggggttcccc cgcacgcacc
     1861 acttcgccca ggccaggtcg gtcttcgtgg tcgcctcgca tcgatgtgat gaacggtttg
     1921 atccactcgg cgatgtcgca gcgacgagtg tgaccttgtg gcggctcccg gctgagattg
     1981 aggaagcgtt tgacgcccgc tgggaggagt ggctcgacaa cgcagaggac tggaccgatc
     2041 tattcgttcg tctgcagaag ccgcaggatg atcttgtgaa tgtactcctc gccgaggatg
     2101 cggtggctct ggtagacgtg gacgctcatg cccggggggc acgtcgtgtc gatggtcgtc
     2161 gcgtctgtgt ctccaatgca ttctcccgct cggactctga cgttgctcag ctggcccttg
     2221 gcttcgcgca tggcgcttcc ggagagctcg tcgttcccta tgcggggttg tcggcgtgac
     2281 cgagagagcg aacgtcgcct cgtcgttcac cgtcgtcaag ggcgcgatga tcgacgaaac
     2341 gtatgcggtc atggccggct gggacttcga gctgtccaag agggccaacc tggaccagct
     2401 gcgagccgaa aacttcatcg gcgccaagag cgtgacctgg ctgcgcgacg tggccaaggt
     2461 gattaaccgg cggttcgacc ctgccggccg cgaccgcccg ctcgtgcatc tcgcgaagag
     2521 cgggtgtgac ctcgcggagt ggaagccact gctgctatgg cacatgaccc gcgatgagtt
     2581 cttggtccgg gactttctgc agaactggct ctacgcggaa tacgaggacg gcgcgttcca
     2641 ggtcaggccg gccgatgtgc tcggatacct ggccgaaatc aacaaacgtg gtggccagac
     2701 ggaacacgac tggagcgagg cgacgaccaa acgagttgcc agcggtctgt tgcgtatcgc
     2761 ggcggacttc ggactgttgc gcggtgccgg aacggccaag gagttcgcct cctaccacct
     2821 gcccgagcgc agcttcctct acattcttca cgcgctgcgc gatcagcggg tccagcctgc
     2881 caagatcata accgcgagtg actggcggat gttcctgatg gcgtcgacgg acgtggagca
     2941 ggagatctat cggttgcacc agttcaagaa gctggactac caggttgcgg gcagtatcgt
     3001 gcaactctcc cttcccgagg gcagttcggc cggatatgcg cagaggatgg tggcgtgaga
     3061 acccttgagg agcgcctgcg gggctcactc gagccgatcc tcgagcagat cgaccccgcg
     3121 cgcgcggggt taagcaccta cagcgacatg ccatgcgccc tgtttgtcta cgacccagag
     3181 gaagagttcc gccttcgaac tcaggtcgcc atgctggaga cgcgattgtc ccagaagggc
     3241 aagcgcattc gacgcatatc tctcgccgag tgtttagacg aggccatgcg ctcccagcgc
     3301 ccgcttgacc aatggttcga tgcagaacgg tcgaccggcg tggaggttgt ggtagagacg
     3361 gtccatgccg ttctctggga gtactcgccg ctggttgacc acgtcgcggc caggatgccc
     3421 gaggaccctg acccgacccg agatgtcgtg ctgatcctcc gcgccggcgc gctgttcccg
     3481 atgtaccgaa cctcggcgct actcgagcag ctcaagggcc acgtccacgt gccgacggtg
     3541 ctcttctacc caggccgtct ggacggtggg tcagggctgt ctttcatggg cgtacttgat
     3601 cccgaacaca attaccgact gaaggtcttc tgataggggc gatgccttat gacgatcagg
     3661 accctcttcg caggggacat cgaccggaag atcgaagaag tcatcaaggt tgaccagacg
     3721 aacgagcagg tcatccgcga cgagctcaac gagtatgtcg tcaccgacgc gatccgtaag
     3781 tactacctgg agatcctcga cgcgtaccag gagacgccga acaagccgca tgaaggtatc
     3841 gcgatctggg tatcgggctt cttcggctcc ggtaagtcga gcttcgctaa ggtcctcggc
     3901 ctggccatcg agaaccgcag catcgacggc aagggtgctg cggagcggtt cgctgaccga
     3961 ggcggggaca accggctgcg cgtgctgcta cggacgatca gcgagcgtat cccgacccac
     4021 acggtgatct tcgacgtatc caccgaccgg ggcatccgca gtggcaacca gtccataacc
     4081 gagatcatgt accggcagtt cctgcacagc ctcggctacg cgaccgacct cgacctggcc
     4141 gagctggaga tcacgctgga ggagaagggc gagctgcaga agttcgcggc ggagtacgcc
     4201 aagctgttcg gcaagaactg ggatgacgag aagggaaagg tcgccttctc ggccagcgag
     4261 gccagccagg tcatgcacag ccttctgccg cagaccttcc cgatggccga ctcgtgggtg
     4321 cgtgccgcca agagtcgagc agacatcacg cccggcaagc tcgcggagcg ggcgaccgaa
     4381 ctcatgaagc gccgtcgacc cgggtacacg ctcctgttcg tcatcgacga gatcggtcag
     4441 ttcgtggccc gcgatgtgca gaagatgctc gacctacagg cagtcgtcca gcagttgggc
     4501 gtcaagggcc gcggcaagca ttggatcgtc gtcacctccc aggagaagct cgacgacctg
     4561 gtgagcgggc tggagggaac acggattgag cttgcccgcc tgcgggaccg gttcccgctg
     4621 caggtccacc tcgaaccgtc cgacatctcg gaggtgacca gcaagcggat tctcaccaag
     4681 aacgccgctg ccgagacacg cctgggcaag ctgttcgatg a
//
LOCUS       DASRJQ010000240         4175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_705094, whole genome shotgun sequence.
ACCESSION   DASRJQ010000240 DASRJQ010000000
VERSION     DASRJQ010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705094"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1523
                     /locus_tag="VFB89_05480"
     CDS             <1..1523
                     /locus_tag="VFB89_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZM26784.1"
                     /translation="NLDVAAAAILFGLGPAWLGLLWPLLTDPVLPGIPLGSTLLCALP
                     LGLAIAVGRNRLFDDTRLGRGLRDLQVRLLLERDLATASADLVQYLSETFDVTTVMVR
                     SADGGSPRVLAATGPVPDAWRDAPLGAGVQRTTEPPVVGFPLADDAGLLGEIRLAGKV
                     QGAFGSREIGSLARVARAVAVVLRSKLADQRLRAAAGEVAHLASTFSQAGAKLEQATR
                     SVSASVREATDGARLHLADLGQVEGAVKGTAGVVAEVRAEAGRSTDLGRTVEAEGEAL
                     IKSSGQLADEIERSMHALGLVRGEVDALVARGDEIQRISAAINGIAFQTNLLALNAAI
                     EAARAGAEGQGFAVVAEEVRQLAEDTGHSARDIGRLVTGIRDEIERAVSALARVLDDM
                     RDAASRGRAGGVLFEASHQRLNVLLKSAQVIRDRADRLEAATGSLEAAIARSNHAARA
                     QLARAEGAALTIDQQVSTATELRGSAESLARLGDHLATMLAGQSSAPADNNGSRHH"
     gene            complement(1546..>4175)
                     /locus_tag="VFB89_05485"
     CDS             complement(1546..>4175)
                     /locus_tag="VFB89_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZM26785.1"
                     /translation="HTAGAFPFSMVAPGAWIRSIFPAMHPTRNHLLPPDPEAYRTAAH
                     PRGRIIVIAPTRAACETIELALGLHLDTLLQREHGDELREWAARGKGFGIVAGTGVGK
                     TLGIRPIAETILAEPLRVGVVNREREATPETPSWNVVIVTTGIARRWFEDGLITGRDM
                     LVVDEIHQTSAELELCLALGKRARCRFIWLSATVDPSFYARYLDSADVLITDAFDPKF
                     RASVKVVPLRPDAFLDDRFIRHVIKEKRGVAVFVPTRAEVEELAAGLGEQWKRLTTAF
                     YHGGEPIRVIRPFLEGEVVHPWVLAMTAAGQSALNLRGLDTVVIYDARYGNVVDRGRN
                     VLQRMYLGANEILQMAGRVHGRVAGSEVYILSDRSLEFDKLRPGPPEFQLAGDAERVA
                     VTCAALGVDASNLDLPVPLDRVAYRRAVATLTSRGLIESGRLTVYGKQVEAMPVERPW
                     GELLFHAEADLIPIVAVCSNIESLHRMTREERDLHGALVNGSDHLTAYNLYAEAVNQH
                     GYLGEVYGLPRHLFDEEGLAAWAEARGVLIKAVEDAALGTASVYRSLELPLPARLPYA
                     SRELRQRWAELIARIMPFELVIDERTADGQEARVSKTSVAGSWGAVAGNLRYFADRFG
                     IARAGIEGTTLPYELVRRHATWGAHSISVGGPRRHQGLLVSRGLSYFGFDLDAETEPL
                     EGEFPSSMREQARDALAGALLTGSTIHPDQGRIRRAMTELEDLWRRSGGRLVALDRSR
                     LHNIVRAQLERVTSWDSFLATRVTLNPLELVDPDMRSRLAELPASVRVKGDVVALEYD
                     IEDGTAVARLRLREGQARRLQLRDLPVLDRPLRFEVIRGRHPPVRAASLPDLQRELNR
                     LARRDPRFRGKTKRGRFRH"
BASE COUNT          734 a         1374 c         1343 g          724 t
ORIGIN      
        1 gcaatctcga tgttgccgct gccgcaatcc tgtttggcct ggggccggct tggttgggtc
       61 tactctggcc cctgctcacc gacccggtcc ttccgggcat cccgctcggc agcacattgc
      121 tttgcgcgct ccccctcggc ctggcgatcg cggtgggccg gaatcgactg ttcgacgata
      181 ccaggttggg tcgcggcctt cgcgaccttc aggtgcgtct gctactggag cgggatcttg
      241 cgaccgcgtc ggcagatctt gtgcagtacc tctccgagac gttcgatgtg acaacggtga
      301 tggtgcgctc agctgatgga ggctccccac gcgttctggc tgcgacaggc cctgttcccg
      361 acgcatggcg tgacgctccc cttggcgcgg gtgtccagcg aacgaccgag cctccggtgg
      421 tcgggttccc tctcgcggac gacgctgggc tattggggga gatcaggctg gccggcaagg
      481 tccagggggc ctttggctcc cgggagatcg ggtcgctcgc acgggtggcg cgggccgttg
      541 ccgtggtgct tcggagcaag ctcgccgacc agcgactccg agcggcagcc ggtgaggtcg
      601 cgcacctggc gtcgaccttt tctcaagccg gcgcgaaact ggagcaagcg acccgatcgg
      661 tcagcgccag cgttcgggaa gcgaccgacg gggcccggct tcacctggcc gacctgggtc
      721 aggtggaagg ggctgtgaag ggcaccgccg gcgtggtggc agaagtccgc gccgaagcgg
      781 gacgcagcac cgatttagga cggacagtgg aggcggaagg cgaagcgctg atcaagagct
      841 ccgggcagct ggccgacgag atcgagcgca gcatgcatgc gctgggtttg gtgcgaggcg
      901 aggtggacgc cctggttgcg cgcggagacg agattcagcg aatcagcgcc gcgatcaacg
      961 gcattgcgtt ccagaccaac ctgctggcgc tcaacgccgc gattgaggcg gcgcgcgcag
     1021 gcgccgaggg tcagggtttc gccgtcgtgg ccgaagaggt ccggcagttg gcggaggata
     1081 ccggccactc ggcccgtgac atcgggcgcc tggtaaccgg cattcgagat gaaatcgagc
     1141 gcgccgtgtc cgcactcgcc cgcgtcctcg acgacatgcg cgatgccgca tcgcgcggcc
     1201 gcgccggcgg ggtgctgttc gaagcctcgc accaacgctt gaatgtgttg ctgaagtcag
     1261 cgcaagtcat ccgagatcgc gctgatcgcc tcgaagcggc aaccggaagt ctcgaggccg
     1321 ccatagctcg cagcaatcac gccgcgcgag cgcagctggc tcgtgccgaa ggcgcggccc
     1381 ttacgatcga ccagcaggtc tcgacggcaa ccgagctgcg cgggagcgcc gagagtctgg
     1441 cgcggctggg cgaccaccta gccacaatgc tcgcgggtca atcctcagcc cccgccgaca
     1501 ataatggctc ccggcaccac tgagcttcct tcacgccgaa attcttcagt gtcggaaccg
     1561 cccgcgcttc gtcttccccc tgaaccttgg atcccggcgt gccaatcggt tgagttccct
     1621 ctgaagatcc ggaagggagg cggctcgaac cggcgggtgt cggccgcgaa tgacctcgaa
     1681 gcgaagcggc cggtcgagca ctggaaggtc tcttagctgc agtcgccgcg cctgcccttc
     1741 acgcaggcgg agtcgggcga cagccgttcc gtcctcgatg tcatactcca gggcgacgac
     1801 atcgcctttc acacggaccg acgcgggtag ctccgccagg cgggatcgca tgtctgggtc
     1861 gaccaattcg aggggattga gcgtcactcg cgtcgccaaa aagctgtccc agctcgtgac
     1921 ccgctcgagc tgagccctga cgatgttatg caatcggctg cggtcgagtg cgacgagcct
     1981 gccgccggag cgacgccaaa gatcctccag ctcggtcatc gctcgacgga tccgtccctg
     2041 atcagggtgt atcgtgctac ctgtcaggag cgcaccggcc aaggcgtcgc gcgcctgctc
     2101 ccgcatactc gaagggaact cgccttccag cggctccgtc tcggcatcca gatcgaagcc
     2161 gaaatagctc aagccgcgtg atacgaggag gccttggtgg cgtctcggcc ccccgaccga
     2221 gatactgtgg gcgccccacg tcgcgtgccg acgcacaagc tcatagggca gtgtggtgcc
     2281 ttcaatccct gccctagcga ttccgaagcg atcggcgaag taacgcagat tcccggcaac
     2341 agcaccccaa ctacccgcaa ccgacgtctt cgacactcgc gcttcctgac cgtcggcggt
     2401 tctttcgtcg atgaccaact cgaaaggcat gattcgcgcg atcagctcgg cccatcgctg
     2461 ccgcagctcg cgtgacgcat agggtagcct cgccgggagg ggcagctcga gggaccggta
     2521 taccgaagct gttcccagcg ccgcatcctc taccgccttg atcagaactc cgcgcgcctc
     2581 cgcccaggcc gcgagccctt cctcgtcgaa caggtggcgc ggcaatccgt agacctctcc
     2641 gaggtatcca tgctgattta ctgcctcggc atagagattg tacgcagtga gatggtcgct
     2701 cccattcacc agcgctccat gcaggtcgcg ctcctcgcgg gtcatccggt gaagcgactc
     2761 gatatttgag cagacggcga cgatcgggat gaggtcagcc tcggcgtgaa acagaagctc
     2821 accccagggg cgctccaccg gcatggcttc cacctgtttg ccatatacgg tgagccgtcc
     2881 gctctcgatc aaccctcgtg atgtcaacgt ggcgacggca cgccggtacg cgacccgatc
     2941 cagcggaact ggaagatcca gattactcgc gtctaccccg agcgctgcgc aggtgaccgc
     3001 cacccgctcc gcatcgccgg ccagctgaaa ttcgggcgga ccgggacgaa gcttgtcgaa
     3061 ttcgaggctg cgatcgctca gaatgtagac ctcgctcccg gcgacgcgtc cgtgaactcg
     3121 cccggccatt tgaaggattt cgttggcccc cagatacatc cgctgaagca cattgcgacc
     3181 ccgatccacc acgttgccgt agcgcgcgtc gtagatcacg accgtgtcga gcccccgcag
     3241 attgagcgcg gactgacccg ccgcggtcat ggcgaggacc cagggatgca ccacttcgcc
     3301 ttcgagaaat ggtcgaatga ccctgatcgg ctctcctccg tgatagaacg cggtggtaag
     3361 ccgcttccat tgctcgccca atccagcagc aagctcctcg acctcggccc gggtgggcac
     3421 gaagactgcg accccgcgct tttccttgat cacgtgtcgg atgaagcggt cgtccaggaa
     3481 agcatcgggc ctgaggggaa ccactttgac gctggcgcga aatttcgggt cgaacgcgtc
     3541 cgtaatcagg acgtcggcgc tgtccaggta gcgggcataa aagctcggat cgacggtcgc
     3601 gctgagccag atgaagcggc atcgcgcccg cttgcctaac gcgagacaaa gctcgagctc
     3661 cgctgatgtc tgatggatct cgtcgacgac gagcatgtcc ctgcccgtga tcagcccgtc
     3721 ttcaaaccag cgacgggcga tgccggtcgt cacgatgacc acattccagc tcggcgtttc
     3781 cggagtagcc tcgcgctcgc gattgaccac accgacccgc aacggttcgg ccagaatggt
     3841 ttccgcaatg ggacgaatac caagcgtctt gccgaccccg gtgccggcca caatcccgaa
     3901 tcctttgccc cgtgccgccc attcccgaag ctcgtcgccg tgctcgcgct ggagcagcgt
     3961 atcgaggtga agtcccaggg caagctcgat cgtctcgcaa gcggcgcgcg tcggtgcgat
     4021 gacgatgatg cggcctcgcg gatgcgcggc cgttcgatac gcctcgggat ccggcgggag
     4081 caggtgattc cgggtggggt gcatcgccgg gaagatagat cgaatccagg cgccgggagc
     4141 aaccatggaa aaagggaagg cccccgctgt atgcg
//
LOCUS       DASRJQ010000241         2630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3005355, whole genome shotgun sequence.
ACCESSION   DASRJQ010000241 DASRJQ010000000
VERSION     DASRJQ010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3005355"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VFB89_05490"
     CDS             <1..737
                     /locus_tag="VFB89_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26786.1"
                     /translation="TLTSELIALFSLKEVGAPEFATQNAEGKKGGGWDMKLPGLPVYL
                     QFKRAARMVRRTAQEAPAFKSLPFFRMYLHRRDHSDQHQLLLDLESQDNLVVYAAPGF
                     SEADELNEAYSLDLVAARSIFVRPSAIGPLVDDERHWVAFQTTPPLARFCSEPHPIDF
                     VLPDALFRRARAAAEPLRGRPQGSESYGAIAEELLATYERTRRTVVEHDRVAAIRRLR
                     ERRTAPDFVRLVARTLFQCELLILPL"
     gene            852..1079
                     /locus_tag="VFB89_05495"
     CDS             852..1079
                     /locus_tag="VFB89_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26787.1"
                     /translation="MKERVRVNGEWAPAAEVEAYLVGAMGPEIATQLMEAARENAKMT
                     DDEVKAAVKLMWDDLRPRSRHMPLGSRRSAT"
     gene            1226..>2630
                     /locus_tag="VFB89_05500"
     CDS             1226..>2630
                     /locus_tag="VFB89_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26788.1"
                     /translation="MQSPGVHIRTSRLLLGLGTLTACLSGGLAPAPAQAQCALATDPC
                     LTAADVTNVTAAAATAIGRNTMVIAVVDRGGNVLGVFSRPGAPGQVVGNFGAMVPTND
                     YAIGLARTGAFFSNDQAPLSSRTVRFISGIHFPPGVRNKPPAALYGIENTNRGCDFGF
                     NIPNVPRAAALGSVSCTNGTPGNLTCGTGINTGKADLFDSNPSAVDGGGVPIFKNGRV
                     AGGIGVAGVPPESAEYAAFAGSLACANPAFGPRPASPATIFLDGIRLPFVKNMNRPPG
                     ETAGPSPETGAFLPGFPQPSPLGAAGVPSGYLFGPNASAQLTAPQVNSVINRANDQAQ
                     KTRAAIRLPLGSTTRMVIAVAGLDGTILGLFRMPDATIFSIDVAVAKARNAIYFSSQG
                     RDPRDIRGVPQGTAVSARTIGFGAMPLFPPGINDTQPGPFFSTFVQDVATPCSQGLDP
                     ANPNQSGIVFFPGSLALY"
BASE COUNT          469 a          871 c          830 g          460 t
ORIGIN      
        1 atacgctgac cagtgagctc attgcgttgt tctcgcttaa agaggtgggt gccccagagt
       61 ttgccaccca aaacgccgaa ggcaaaaagg gcggcggctg ggacatgaag ttgcccgggt
      121 taccggtgta tctccagttc aagcgcgctg cgcgaatggt tcgacgtact gcacaagagg
      181 cgccagcctt caagagtctc ccattctttc gaatgtatct tcaccgtcgc gaccactcgg
      241 accaacatca actccttctc gatcttgagt cgcaggacaa cctcgttgtg tacgcggccc
      301 cgggcttcag cgaggcggat gagttgaatg aagcctactc acttgatctc gtcgcagcac
      361 ggtcgatctt cgtccggcct tcagccatag ggccgctcgt cgacgatgag cgacactggg
      421 ttgcctttca gacaacgccg ccactcgcgc gattttgttc tgagccgcat ccgatcgact
      481 tcgtcttacc ggatgcgtta tttcggcgag cacgagctgc cgcggaaccg ctacgcggcc
      541 gtccccaggg ctccgagtcc tatggcgcaa tcgcagaaga gctgctcgct acgtatgaac
      601 ggacgcgccg cacagtagtt gagcatgacc gcgtcgcggc gatccgccga cttcgcgagc
      661 gacgcaccgc tccggatttt gtccggctcg tggcgagaac gttgttccag tgtgagctgc
      721 tcattctccc gttgtgacgc cgtaccgtcc aaaggctgcg agtcgccctg atcaagcggt
      781 tcaggttaga gcggatcgcg atcgaagaca ttcatacttc gtgcgcaatc gtcgaccgga
      841 ggattagcgt catgaaagag agggtgaggg tcaacggtga gtgggcgcca gcagccgagg
      901 tggaggccta cttggtcggg gcgatggggc cggaaatcgc cacgcagctg atggaggcgg
      961 cccgcgagaa tgcgaagatg actgacgacg aggtcaaggc ggcagtcaag ctgatgtggg
     1021 acgatctccg acctcggagc cgccatatgc cgctcggttc caggcggtcc gccacctagg
     1081 gctgtgccca gcgctgtgcc caaacccact ccatagccac cggaaaccaa cggacgctac
     1141 cggatgtagg agaaggaaac gccccaggaa ctacacgatt tcctgggggg tcccgcttcg
     1201 cccatacgct aagataaccg gcaccgtgca gtcccctggg gtacacatca gaacgtcgcg
     1261 attgctgctg gggctcggca cgctcaccgc gtgcctcagc ggcgggctcg cgcccgcccc
     1321 ggcccaggcc cagtgcgcgc tcgccacgga cccctgcctc accgccgcgg atgtgaccaa
     1381 cgtcaccgcg gcggccgcca cggcgatcgg ccgaaacacc atggtcatcg ccgtggtcga
     1441 ccggggcggc aatgtgctcg gggttttcag tagaccgggt gcgccggggc aggtcgtcgg
     1501 gaacttcggc gcgatggtcc ctaccaacga ctatgcgatc gggctggccc gcaccggggc
     1561 gttcttctcc aacgaccagg cgccgctgtc ctcgcgcacc gtgcgcttca tcagcggcat
     1621 ccactttccg cccggcgtgc gcaacaagcc tccggccgcg ctctacggga tcgagaacac
     1681 gaaccgcggc tgtgacttcg ggttcaacat tccgaacgtc ccacgcgcgg ctgctctggg
     1741 cagcgtctcg tgcacgaacg gcacgcccgg caacttgact tgcgggaccg ggatcaacac
     1801 gggcaaggcg gacctcttcg acagcaaccc gagcgccgtc gacggcggtg gggtgccgat
     1861 cttcaagaac ggccgggtcg caggggggat cggcgtggcc ggggtgccgc ccgaatcggc
     1921 ggagtatgcc gcgttcgcgg gcagtctcgc gtgtgcgaat ccggcgtttg ggccgcggcc
     1981 agcatccccc gccacgattt tccttgacgg aatccggctg ccatttgtca agaacatgaa
     2041 ccgtccaccc ggcgagaccg ctggaccaag tccggagacc ggcgcgttct tgccgggctt
     2101 cccgcagccg agtcctctcg gcgccgccgg cgtgccgtcg ggctatctgt tcggccccaa
     2161 cgcgagcgcc cagctcaccg cccctcaggt gaactcggtc atcaatcggg ccaacgacca
     2221 ggcgcagaag acgcgggccg ccatccggct gccgctgggc agcacgacgc ggatggtaat
     2281 cgcggtggcg ggcctcgatg gcacgatcct cggcctgttc cggatgccgg acgctacgat
     2341 tttcagcatc gatgtggccg tggcaaaggc ccggaacgcg atctacttca gcagccaggg
     2401 gcgcgatccg cgagacattc gcggggtgcc gcaaggcacg gcggtctcgg cgcgaacgat
     2461 cggtttcggc gcgatgcctc tttttcctcc cgggatcaac gacacccaac cgggaccatt
     2521 cttcagcacc ttcgtgcagg acgtagccac gccgtgctcg cagggcctcg atcccgccaa
     2581 tcctaaccag agcgggatcg tcttcttccc cggcagcctg gcactctacc
//
LOCUS       DASRJQ010000242         3503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_139783, whole genome shotgun sequence.
ACCESSION   DASRJQ010000242 DASRJQ010000000
VERSION     DASRJQ010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139783"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1470
                     /locus_tag="VFB89_05505"
     CDS             <1..1470
                     /locus_tag="VFB89_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26789.1"
                     /translation="PRDLGGSLYRPLTIVRFALQWIAGGGTPFVFHAVNVCLYLALTL
                     SVYLVGRAMMTREAAWLAAALFAVHPVHVESVGNGVGQSELVVTLCLTLGTLIYLRAR
                     RRSELRASESISFFLLTLIAAFSKENGIVLPALLAATEITVVRDPRGWRERVRVLLPT
                     AILAGLAVGIVLAARHWALDALVGEHPFFSLSGLGFGDRVLTVLGIIPIWLRLFIWPA
                     HLQADYGPPDVDVSTSWGVAQTLGLAVLVGAVALVVANWRRLPAVAFGFLWAGLALFP
                     VSNLLVPTGILVAERTLLLPSVGTSLVLGALIYAVRQALPQDRKQLRKLGLAALTGLL
                     VAGVIRSGLRQPVWRDTPTLLAQTVLDAPRDYRAWQLFGNQLASRGHTAQGRIALEHA
                     AALYNKDPGVFEDLGQVVRGSEGCPAAMPIFERTIAMDPHRKNTRTRLYLCLLTTGDT
                     ARAATLAEQGASLGQWYFQLVMALSGGKRSLPLPGDRSQ"
     gene            complement(1478..3361)
                     /locus_tag="VFB89_05510"
     CDS             complement(1478..3361)
                     /locus_tag="VFB89_05510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZM26790.1"
                     /translation="MSVTSVELLPEAPSVVEAVVSPRPPRVWPWTVLVAMVACAAFAQ
                     TYRYAFAYDDIAVIQDRVLFHSLANWRSIITASWWFNNNLYRPLTQLSFAANWSASGG
                     AAPAFHLTNIVLHAITAALVYLLARRLLGAWPALVAGLLFAVHPVHVEAVANLVGRAE
                     VLAALFSITAVMAYLKDGELAEAGDDTSWRRYLTSFGTLVATALALASKESAFALPGL
                     ALLADWFDVGRRGRKSLGVERHWVLWVGIVGVTFGWLLWRSQVVGDLTGLEVAPGLDD
                     AGLAERTLIMLPIVLAYLRLLFLPYRLSADYNPDFIKPASSLTLAGVAGLLVFLLALW
                     LAWAMRRRAPVVTFALGWIGATLAIVANILVPTGVLLAERILYMPSIGAVLLLAAGWD
                     WIYRRRPTPAIVGLIVVLAAGAARTVTRNPVWRNNDTLFPQIIRDAPGSFHSKAMEAI
                     LSERRGDLRSAERQLRQALSIYPLAWGLWKDLGRVLDTQGRFAEASKYWWTAWRLNNS
                     ALVTAQRAIQTALFAGMLDTAEARLAVARAARPHSGELFLAAADVALARNQPRRAMTL
                     RRQVAWEYPDSARFWGLTAEAALKAGECPELLRSIGRLRGLKADSAQIVSLAAGAKRL
                     DCR"
BASE COUNT          631 a         1164 c         1122 g          586 t
ORIGIN      
        1 ccccgcgacc ttggcggctc gctctatcgg ccgctcacga tcgtgcgatt cgcgcttcaa
       61 tggatagccg gcggcggcac cccgttcgtc tttcacgccg tgaacgtctg cctgtatctc
      121 gccctgacgc tctcggtcta cctggtgggg cgtgccatga tgacccgcga agcagcctgg
      181 ctggctgcgg cgctgtttgc ggttcatccg gtccacgtcg aatcggtcgg taacggagtg
      241 gggcaatcgg agctggtcgt cacgctctgc ctgacgctgg gcacgcttat ctatctccgc
      301 gcccggcgcc gatcggagct tcgggcaagc gagagcatca gcttcttcct gctcactctc
      361 attgccgcat tcagcaagga aaacggcatc gttctcccgg ccttgctcgc cgccaccgag
      421 atcacagtcg tccgcgatcc ccgtggctgg cgggaacgcg tgcgggtgtt gctacccact
      481 gcgattctgg cgggcctcgc cgtcggaatc gtgctggctg cgcgtcattg ggcgctcgat
      541 gcgctggtgg gcgagcatcc cttcttttcg ctcagcggcc tcggcttcgg tgaccgggtt
      601 ctcacggtcc tcggaatcat ccccatctgg ctccgcctgt tcatctggcc agcccacttg
      661 caggccgact atgggccacc cgacgtcgac gtctcgacca gctggggcgt cgcgcaaaca
      721 ttgggactcg ccgtgctcgt gggagccgtc gctctcgttg tcgctaactg gcggcgtctg
      781 cccgccgtgg cgttcggatt cctctgggcc ggcctggcgc tgtttccggt gagcaacctg
      841 ctcgtgccca cggggatttt ggtggcagag cgaacgctgc tgttgccaag tgtggggacg
      901 tcgctggtcc tgggagcgtt gatctatgct gtccggcaag ccttgcccca ggaccggaag
      961 cagctgcgga aactgggact tgcggcgttg accggtttgt tggtggcggg agtgatccgg
     1021 agcgggctgc gccaaccggt gtggcgcgat acccctacct tactcgcgca gaccgtgctg
     1081 gacgcgccgc gggactatcg agcctggcag ctctttggta atcagctcgc gtcgcgcggg
     1141 cacaccgcac agggccgcat cgcactcgaa cacgccgcgg cgctgtacaa caaggatccg
     1201 ggcgtattcg aggatctcgg ccaagtggtt cgagggagcg agggatgtcc tgccgcgatg
     1261 ccgatcttcg agcggaccat cgcgatggac ccccatcgga agaacacccg cacccgcctt
     1321 tacttgtgcc tcctcacaac cggcgacact gctcgcgctg ccacactcgc cgagcaaggt
     1381 gccagcctgg gtcagtggta cttccagttg gttatggcgc tgtccggagg aaagcgatcg
     1441 ctaccgcttc cgggagatag atcgcagtag cgtgggttca gcggcagtcg agccttttgg
     1501 ctcccgccgc aagcgagacg atctgagccg agtccgcttt gagcccgcga agtcggccga
     1561 tcgaccgcag cagctcagga cattcccctg ctttgagcgc cgcctccgcc gtcaaccccc
     1621 agaaacgagc cgagtcggga tactcccagg cgacctgacg gcgcagcgtc atggcccggc
     1681 ggggctggtt tcgcgcgagg gcaacatcgg cggcggcgag gaatagctcg ccgctatgtg
     1741 gacgggctgc ccgagccact gccagtcgcg cctcggcagt gtccagcatt ccggcaaaaa
     1801 gagcggtctg aatcgcgcgc tgcgcagtca caagcgcgct attattgagc cgccatgccg
     1861 tccaccagta tttgctcgcc tcggcgaacc ggccctgagt gtcgagcacc ctgccgaggt
     1921 ctttccacag gccccaagcg agcggataaa tggaaagcgc ctggcgaagc tgccgttccg
     1981 cgctgcgtag atccccgcgg cgctccgaca gaatggcttc catcgccttg gaatggaacg
     2041 atcccggtgc gtcacggatg atctggggga atagggtgtc gttgttccgc caaaccggat
     2101 ttcgggtcac ggtccgagca gcgcccgcgg ccaggacgac gatcagcccg acgatggcag
     2161 gcgtcggccg gcgtcggtag atccagtccc atccggcagc cagcagcagt acggcgccga
     2221 tcgacggcat atacaggatc cgctccgcca gcagcacgcc ggtcggcacc aggatgttgg
     2281 caacgatggc gagggtagcg ccgatccagc ccagagcgaa ggtgaccaca ggagccctgc
     2341 gcctcatcgc ccaggcgagc caaagagcca gcaggaaaac gagcaggccg gctacgcctg
     2401 ccaatgtgag actcgaggcg ggcttgatga agtcggggtt gtagtcggct gaaagacgat
     2461 agggcaggaa cagcagcctc aggtatgcca gcacgatcgg cagcatgatg agggtccgct
     2521 cggccaaccc ggcgtcgtcc agcccgggcg cgacctcgag ccccgtgagg tcgcccacga
     2581 cctgcgagcg ccacagcagc cagccgaaag tcactcccac gatgccgacc cagagcaccc
     2641 agtgccgctc cacaccgagg ctcttccgac cgcgccgccc cacgtcgaac cagtcggcca
     2701 gcaatgccag tcccggcaat gcgaaggcag actccttgga ggcgagcgca agagccgtcg
     2761 ctacgagcgt cccgaacgat gtgaggtacc gccgccagga cgtgtcgtca ccggcctccg
     2821 ccagctcccc atcctttaaa taggccatca ccgcagtgat gctgaacagg gcagcgagca
     2881 cctcggcccg cccgaccaga ttcgcgactg cctcgacgtg cacgggatgc accgcaaaca
     2941 gcaatcccgc aaccaacgcg ggccaggccc ccagcagccg ccgagccagc agatagacca
     3001 gggcggcagt gatcgcgtgc agaacgatgt tggtgagatg aaaagcaggc gcggccccgc
     3061 cgctcgcgct ccagttagca gcgaagctga gttgggtcag cggcctatag agattgttgt
     3121 tgaaccacca gctggcggtg ataatggagc gccagtttgc cagcgaatgg aagagcacac
     3181 gatcctgaat gaccgcgatg tcgtcgtaag caaaggcata gcggtaggtc tgagcgaacg
     3241 ctgcacacgc aaccatggca acgaggacgg tccacggcca tacccgcggg ggacgggggg
     3301 acaccacagc ctcgaccaca ctcggggctt cgggcagaag ctcgaccgat gtcactgaca
     3361 tcgcagctcc tcggcactcc tctcgagcgg ggcaaccaac gccgcctccg ccgacaaagc
     3421 tcgcaaccga ccgagcgagt tcaaaacctc gggacactgc tgtgcgccgg cagccgctgt
     3481 tccggtgagc gcccaatagc ggg
//
LOCUS       DASRJQ010000243         2582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3891333, whole genome shotgun sequence.
ACCESSION   DASRJQ010000243 DASRJQ010000000
VERSION     DASRJQ010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3891333"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..282
                     /locus_tag="VFB89_05515"
     CDS             64..282
                     /locus_tag="VFB89_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26791.1"
                     /translation="MSLRSIAIALVGIGVAAFVVNYLGGIEGRCSEDGWSGCGWTWQL
                     SGWVVLVCFAGLVGLGVSALARCVRRRP"
     gene            complement(760..1203)
                     /locus_tag="VFB89_05520"
     CDS             complement(760..1203)
                     /locus_tag="VFB89_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26792.1"
                     /translation="MYAKQVGLIALMALGSVSLWVANPLLWFWLTSHLQSQAGQAQMG
                     PYVLLLIGVIFTAVVIGKGLAALNRRYGRVIGAEPTVRIIVPWRRSLRDARHGGREDP
                     GRVPVSILDVVMVLSVLAAVIAFALWYVVTDPTPPNVGGPGPSKD"
     gene            1230..1895
                     /locus_tag="VFB89_05525"
     CDS             1230..1895
                     /locus_tag="VFB89_05525"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZM26793.1"
                     /translation="MDNRAATARLTRAEQQARTRAALIEAAGRVFIERGFAGSSVEAI
                     AAEAGFTRGAFYSNFRSKEELFAELLQVRVYSLYADLARQSADRATRTSPRDTGERLA
                     AIQANPDGRWIFRLWLELLAHAGRDDEFRRIAAGFWSGNRALAASAIATAYEEADTTP
                     PIAPNRLASAWIAIDIGLALQHFVDPEGAPLELYPELCELLFGPLNPAPKRTTQVKAT
                     ERQ"
     gene            complement(2210..>2582)
                     /locus_tag="VFB89_05530"
     CDS             complement(2210..>2582)
                     /locus_tag="VFB89_05530"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZM26794.1"
                     /translation="EAFNRRDFDAVLINYHREVQLIPPAALVEAGAVESSYRGHDGYR
                     RYFREWLSAWGAYRGETQELIDLGDRYLTLGWLEGRGGGSRVPVTQEHASLMIMRDGL
                     VIRQQEYWSRAEALEAVGLQE"
BASE COUNT          440 a          855 c          882 g          405 t
ORIGIN      
        1 ggaggctgtc gggaaaaggg ggtgcttcac tggtgagtca aggtggttgg tgacaatgcc
       61 cccgtgagcc ttcggagcat tgcgattgcc ttggtgggca tcggtgtcgc ggcgttcgtg
      121 gtgaactacc tcgggggcat cgaggggcgc tgttcggagg atggctggtc gggctgtgga
      181 tggacgtggc agctatcggg ctgggttgtt ctcgtctgtt tcgctggatt ggtggggctg
      241 ggtgtgagtg ccttggcgcg gtgcgttcgg cgccgccctt gacagtcagc tcgggcggtg
      301 gaaatcggca cacgcgtgac gtccgccgcg tctaatactc gcggcatggc gcaatttgct
      361 tgccgtggga tggcgagcga tcgcttctgt gttggtcgga ggctctcgag aagagggtgc
      421 tgtccggcgc gtcaggtcgg agtggcggct gttcggcgca agccacaacc tgctcaagct
      481 cacaaccacc ggacaggcgc cgccgcggcc tgaaagcccg gcggccaatc cccacgccaa
      541 ggcgagcccc ccgaggccac gacgccccga aggtccgcgc acccctttaa ccgacagcct
      601 cgcgcaaagc agcagccctg ataggcgagg gccgcatagt gcctcgcatg ggaagcgaaa
      661 agcgaaaccc accgccaaca ggctttagcg cgtcgcgcag agcagcacac tccggctgaa
      721 tcgcggagcc gggagcgggc agtgcgaagc agccgagact cagtctttgg atgggccggg
      781 gccgcccacg ttgggcgggg ttgggtctgt gaccacatac cagagggcga aggcaatgac
      841 ggcggccagc acagagagca ccatcacaac gtcgagaatg cttaccggga cgcggccggg
      901 gtcctcgcgg ccgccgtggc gggcgtcgcg caggctgcgt cgccagggaa cgatgatccg
      961 caccgtgggc tccgcgccga tgacgcggcc gtagcggcgg ttgagcgcgg ccaggccctt
     1021 cccgatcacc acagcggtga agatcactcc gatcagcagc aggacgtacg ggcccatttg
     1081 ggcctggccg gcctgcgatt gcaggtgcga ggtgagccag aaccagagca gcgggttggc
     1141 gacccagagt gagacgctgc cgagcgccat cagcgcgatc aggccaacct gcttcgcata
     1201 cataggcgta tccaaataca ctattgcata tggataatcg ggccgccacg gcgcgcctga
     1261 cgcgcgccga gcagcaggcg cggacgcggg ccgcgctgat cgaggcggcg ggccgcgtct
     1321 tcatcgaacg cggcttcgcc ggcagcagcg tggaagcgat cgcggcggag gcggggttca
     1381 cgcgcggcgc cttttactcg aacttcaggt cgaaggagga gctgtttgca gagctgctcc
     1441 aggtgcgggt gtacagcctg tacgccgacc tggccaggca gagcgccgac cgcgccacgc
     1501 gcaccagccc gcgcgacacc ggcgagcggc tggcggcgat ccaggctaat cccgacggcc
     1561 gctggatctt ccgcctctgg ctagaactgc tcgcgcacgc cggtcgcgac gacgagttcc
     1621 gcagaatcgc cgccggcttc tggagcggca accgcgcact ggccgcgagc gccatcgcaa
     1681 cggcctacga ggaagccgac accactccac caatcgcacc gaaccgcctg gcgagcgcct
     1741 ggatagccat cgacatcggc ctcgccctgc agcacttcgt ggaccccgag ggggcgccgc
     1801 tcgagctcta ccccgagttg tgcgagctgc tcttcggacc gctgaacccc gcaccaaagc
     1861 gcacgacgca agtcaaagcg accgagcgcc agtagcgagt gcggcgacct cgacttgctg
     1921 gttggacgaa aagccggcag ccaggtggca gccaaactcc gagatcgctc tcgtcccagc
     1981 gacctacggt tgagcgtccg cgccactgac cgccccccac cgcgggggtg tggtccaggc
     2041 ggtggcggat tcgacccgtc gctcatccct caggacgtcc cgatacacgc gctgcttcgc
     2101 gcaccacggc gccgcggtgc gcgggccgcg ggcatcaata ggggcatcag ttccgacgcc
     2161 tccggtcagt cgtcgtgccc gaaccctccc gcagccgcgt gggcggcgct tactcctgca
     2221 gccccacggc ttcgagggct tcggcacggc tccagtactc ctgctgacgg atcacaaggc
     2281 cgtctctcat gatcatcaga gatgcgtgct cctgggtgac cggaactcga ctgcctcccc
     2341 ctcggccttc cagccatccg agtgtcaagt atcggtcgcc gaggtcaatc agttcctgcg
     2401 tttccccgcg atatgcgccc caggcgctca gccattcgcg gaagtacctg cggtagccgt
     2461 cgtgcccgcg atagctcgac tccacggcgc ccgcctcaac caatgcggcg ggcgggatga
     2521 gttgcacctc gcggtgatag ttgatcaaga ccgcgtcgaa gtcccggcgg ttgaaggcct
     2581 cg
//
LOCUS       DASRJQ010000244         6505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1657635, whole genome shotgun sequence.
ACCESSION   DASRJQ010000244 DASRJQ010000000
VERSION     DASRJQ010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6505
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1657635"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2214
                     /locus_tag="VFB89_05535"
     CDS             <1..2214
                     /locus_tag="VFB89_05535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZM26795.1"
                     /translation="EANEAFLDLVGYSRTEMLEGRLHWKAISPPEHAEADERALRELA
                     DTGVVRPFEKELFRKDGTRVPVVVGGAVTGHEPLEWTSFILDASERKRAEAEILASET
                     RYRSLFERIPAPMWVYDFESLRFLDVNQAAIDQYGWTREEFLRMTIRDIRPPEDLPAF
                     DERLRRRAPGLTLGGRFRHCRKDGSRIEAEVSSQEVLYGGMRSRLVLATDITGRVRMQ
                     EDVQRFVSLVDNAGDFIAMVAPDWRVLYLNKAGRDLIGISEESLAAGISLMSFWHEDS
                     RATITRDVLPRLWQGESWDFDGQLRHVETGEPIAVECNAFGIQDAHSGRLLAGAFVLR
                     DLTERKRVEEHLRQAQRMEAIGRLAGGVAHEVNNMMTVILGFCTFLTKSLDESDERHQ
                     DVAQIARAAERSAEVSRQLLAYSRRQLLQPRVLELNSVLMDMESVLRRLMGEDREVAL
                     RLGGDLNWIKTDRTQLEQVILNLALNARDAMPQGGRLAMETSCVVLDSEYAHRHPATI
                     VRPGPYVRLIVTDTGRGMDPDTQAQVFDPFFTTKPVGHGTGLGLSMAYGIVKQSGGYI
                     WVYSEPGQGTVFKIYLPQVEQTGESANWKPVEVPPRGAGETILVVEDEDLVRGFACRY
                     LRNEGYVTQESTDGERALELLAGEANAVDLVLTDAVMPRMGGRELADHVARLRPGLPV
                     LFMSGYTNDEIIRRGLLSPGVPFLQKPFSPEALANKVREVLDASRGQRTEAAVVS"
     gene            2214..3239
                     /locus_tag="VFB89_05540"
     CDS             2214..3239
                     /locus_tag="VFB89_05540"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZM26796.1"
                     /translation="MRCLVVDDEAIVRKSVVRMLEAQGFTCLEAGSGREGLELLADNG
                     ELPLIISDLRMPELDGIGFLSEVRRQFPDTAVVMLSGMAETNVAVECLHLGAADFLVK
                     PVSVSEMHARVARALEKRSLVMQNRYYQAHLERRIQEQAQRIRELFLEGVQMLARAME
                     AKDAYLRGHSVRVSRYAVETGRLLGLDPSELEQIRIGGELHDIGKIGTRESLLQKPGS
                     LTEDERHHISEHPVLGERMLAPLARESPAVLRIVRSHHERLDGSGFPDGLRGNDIPIE
                     ARIVAVADSFDAMTSERPYRDPRQPDDAIDELRRVAGSQLDPEAVEAFSIAFRDLGAL
                     PVGSEPG"
     gene            complement(3236..4552)
                     /locus_tag="VFB89_05545"
     CDS             complement(3236..4552)
                     /locus_tag="VFB89_05545"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZM26797.1"
                     /translation="MIAIPSQRWLAVGACLALIGLAGLIWPGALGLLLFLDALWVAAL
                     AFDAVAAADSFLLQVERNAPPAFSVGRSFPVRYRWRHAKRRRLTILVQETLPAPLGLA
                     TGVRRLALAPAISTTEELLMRPVSRGRGKGGRMTLRVLGPLGLAWWQRRLELPWDVTV
                     FPSLVGSSLRALPPQARRRRQAGLRSVQRRGEGRLFEGLREWVPGDDTRIIDWKATAR
                     RGKPIARQYEDERREHLLILIDAGRQLTAEIDGVPRIEAAIDAAVRLAHAAVEHDDNV
                     GVMIFADVIQSFVPPTRGRRGLRGVLGALAAAEARLVEPDYPAAFRYLAVRNRRRALT
                     VFFTDVIDRTASDAVVGQVASLRGRHVPLAVTLRDPALERLAVRRPLNEQRAFERAAA
                     EELLLAREDALGEMRRRGILVLDVLPAAAAEAVVERYHQLKRRGVL"
     gene            complement(4549..5532)
                     /locus_tag="VFB89_05550"
     CDS             complement(4549..5532)
                     /locus_tag="VFB89_05550"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZM26798.1"
                     /translation="MGGPEAIGTVGDIGRHCKAANDLVAALGGVVLGQEPVIREAVAA
                     LIARGHVLLEGVPGTAKTLLVRSLSLSLGLEFRRIQFTPDLMPSDITGVNQLTALGTF
                     SFRAGPIFGDLILGDEINRAPAKTQAALLEAMQERSVTVDGVSHPLSPAFTVFATQNP
                     IEFEGTYPLPEAELDRFLVKIVLGYPAAETEQGVLHRVLSGFEADDPGSYGIEPGVGG
                     VGLADLRAAARQVRVEASLVRYVTTIVRATRTTPSLTLGASPRGSVALLKLAQATALL
                     DGRDYVVPDDVKSVAPAVLRHRISVAPELELEGVTPDAALAAILEQVEAPR"
     gene            complement(5511..>6505)
                     /locus_tag="VFB89_05555"
     CDS             complement(5511..>6505)
                     /locus_tag="VFB89_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26799.1"
                     /translation="SDTGNPVLAVVGPTEEIDPLEAAAIARFGTRHDLLLAGEGAEAA
                     MRCYGYVVETRLQPMLAITPGQPMSASDLRVGAVLARAQPRPRRRPTAWDDEGYAVCD
                     TPTTLRADTLLRTVGGRVVALRLEIVGGRAVTLVADDLPFSNRALRSSGAGPFSLRLV
                     AGRYDRIIFDEYHHGYGPSGSLAGSVLGWSTRSPWGWAGWQLAGIGLVALAAAAVRFG
                     PVRPSPERRRRSSLEHVRALATVLSAAEGHDLAIQLMVQGLRRRLSRHPHAAEAGLRA
                     DPRPWVRELTRRARSPRAREALTSLSDLTTHPQSGEGVLQAANVVEEVWEDLKP"
BASE COUNT         1204 a         2139 c         2128 g         1034 t
ORIGIN      
        1 gaagccaacg aagccttcct cgatctggtc ggctattccc gaacggaaat gctcgaaggc
       61 cggctgcact ggaaggcgat cagcccgccc gaacatgccg aggccgacga gcgtgcgctc
      121 cgcgagctgg ccgacaccgg agttgttcgc cccttcgaga aggagttgtt ccgcaaggat
      181 ggcacccgcg ttcccgtcgt ggtcggcggt gccgtgactg ggcacgagcc cctcgagtgg
      241 acctccttca ttctggacgc gagcgagcgg aagcgggccg aggcggaaat cctggccagc
      301 gaaacccgat atcgcagcct gttcgagcgc atccctgcac ctatgtgggt ctacgacttc
      361 gaatcgcttc gctttctcga cgtcaatcag gctgcgatcg accagtacgg ctggacgcgc
      421 gaagaatttc tccggatgac gatccgcgat atccggccgc cggaagatct ccccgccttt
      481 gacgagcgac tgcgccgcag agcccccggc ctcaccctgg gtgggcggtt tcgccattgt
      541 cgcaaggacg gcagccggat cgaggcggag gtctccagtc aggaagtcct ctatggcggg
      601 atgcggtccc ggctggtact ggccaccgat atcacgggtc gcgtgcggat gcaggaagac
      661 gtccagcggt tcgtgtcgct ggtggacaac gccggcgact ttatcgcgat ggtcgcccct
      721 gattggcggg ttctgtacct caacaaggcc gggcgcgatc tgatcggtat cagcgaggag
      781 tcgctggctg cggggatttc gcttatgagt ttctggcacg aggactcgcg cgcaaccatc
      841 acccgcgacg tgctgccccg gctctggcag ggtgaatcct gggacttcga cggccagctc
      901 cggcacgtcg aaaccgggga gccgattgcg gtcgagtgca atgccttcgg aatccaggac
      961 gcgcactccg ggcgcttgct cgccggcgcg ttcgtgctgc gtgacctgac cgagcggaag
     1021 cgcgtggaag aacatctgcg gcaggcgcag cgtatggagg ccatcggacg cctggcagga
     1081 ggtgtcgccc acgaagtcaa caacatgatg acggtgatcc tcggattctg caccttcctc
     1141 accaagagcc tcgatgagtc ggatgagcgc caccaggacg tcgcccagat cgcgcgggcc
     1201 gcagagcgat cggcggaggt cagccggcag ctgctggcct acagccgccg acagctgctg
     1261 cagccgcgag tgctcgagct caacagcgtg ctgatggata tggagtcggt gctccgccgg
     1321 ctgatgggcg aggaccgcga ggtcgcgctg cgtctgggcg gcgacctcaa ctggatcaag
     1381 acagatcgca ctcagctgga acaggtgatt ttgaacctgg ccctcaatgc tcgcgatgcc
     1441 atgccgcaag gaggccgcct cgcgatggag acgagctgtg tggtgctcga tagcgaatac
     1501 gcccaccggc accccgccac aattgtgcgc cccggcccct acgtcaggct gatcgttacc
     1561 gacacagggc gaggcatgga cccggacact caggcccagg tcttcgaccc atttttcacg
     1621 accaaacccg tgggccatgg taccgggctc gggctttcga tggcatacgg tatcgtgaag
     1681 cagagcggcg gctacatctg ggtctacagc gagccgggtc aaggcaccgt tttcaagatc
     1741 tacctgccac aggtggaaca aaccggcgag tcggcaaact ggaagcccgt agaggtgcca
     1801 ccgcgggggg ccggagaaac cattctggtt gtggaagacg aagacctggt tcgcggattc
     1861 gcctgccgct atctccggaa cgaggggtac gtcacccagg agagtaccga cggcgagcgg
     1921 gcgctggagc tgctcgccgg agaagccaac gccgtcgatc tggtgctcac ggacgccgtc
     1981 atgccacgca tgggcggacg tgagcttgcc gaccacgtgg ctcggctccg ccctggactg
     2041 cctgtcctgt tcatgtccgg ctacaccaac gacgagatta ttcgccgcgg cctcttgagc
     2101 ccgggagtac ccttcctcca gaaaccgttt tcgccggaag ccctggccaa caaggttcgc
     2161 gaggttctgg acgcctcgcg gggacaacgc accgaagcgg cggtggtctc gtgatgcggt
     2221 gcctggtagt ggatgacgag gcgatcgtga ggaagtccgt ggtgcggatg ctcgaggccc
     2281 aggggttcac ctgcctggag gcaggcagcg gtcgggaggg cttggagttg ctcgccgaca
     2341 acggagaatt gccactgatt atctccgacc tgcgcatgcc cgagctggac ggcatcggct
     2401 ttctcagcga agtacgtcgc caatttcccg acacggccgt tgtgatgttg agcggcatgg
     2461 ccgagaccaa cgtcgcggtg gagtgcctcc atctcggcgc cgcggatttt ctagtcaagc
     2521 ccgtttcagt gagcgagatg cacgcgaggg tagcgcgtgc cctcgaaaag cgctcgctgg
     2581 tcatgcagaa tcggtattac caggcccatc tagaacgccg catccaggag caggcgcagc
     2641 gcatccgcga gttgtttctg gaaggagttc agatgctcgc tcgcgcaatg gaggcgaagg
     2701 acgcctactt gcgcggccat tcggttcggg tgagccgtta cgcggtagaa accggccggt
     2761 tgctagggct ggacccgtcg gagctggagc agattcgaat cggtggggag ctccacgaca
     2821 tcggcaagat cggcacccgt gagtccctcc tgcaaaaacc gggttcgctc accgaagacg
     2881 agcgccatca catcagcgag cacccggtgc tcggcgaacg tatgctggca ccgctcgccc
     2941 gcgagtcacc cgccgtgctc cgaatcgtgc gatcccatca cgagcgcctc gacgggagcg
     3001 gatttcccga cggactgcgg gggaacgaca ttcccatcga ggctcgcatc gtcgcggtcg
     3061 ccgactcgtt cgatgcgatg acgtcggaac ggccctatcg cgatccgcgg caaccggacg
     3121 acgccattga tgagttgcgg cgagtagcag ggagtcagct cgaccccgaa gcggtcgagg
     3181 cattcagtat tgcgtttcgt gatctcggag ccctgcccgt cggctccgaa ccgggctaga
     3241 gcacgccccg tcgcttgagc tgatgatagc gttcgaccac ggcctcggcg gctgcggcgg
     3301 gaagcacatc gagcaccagg atcccacgtc gccgcatctc gcccaacgcg tcttctcgcg
     3361 ccagcagcag ctcctccgcg gcagctcgct cgaaagcgcg ctgttcattc agcggccgtc
     3421 gaacggccaa acgctcgagc gccgggtcgc gcagcgtcac ggcgagcggc acatgccggc
     3481 cccgtaagct ggccacctgc cccaccactg cgtcactcgc agtgcggtcg atgacgtcgg
     3541 taaagaaaac ggtaagggcc cggcgccggt tgcggaccgc caggtaccgg aatgcagccg
     3601 gatagtccgg ctccaccagc cgggcttcag ccgccgccaa cgcccccaga accccccgaa
     3661 gcccgcgtcg tccgcgggtg ggcggcacga agctctggat tacgtcagcg aaaatcatca
     3721 cgcccacgtt gtcgtcatgc tccacggccg catgcgccag gcgaaccgcc gcatcgatcg
     3781 ccgcttcgat gcgcggcact ccgtctatct cggcagtgag ctggcggccg gcgtcgatca
     3841 aaatcagcag gtgctcccgt cgctcatctt cgtactgacg tgcgatcggc tttccgcggc
     3901 gcgcggtagc cttccaatcg atgatgcggg tgtcgtctcc cggcacccat tcgcggaggc
     3961 cttcgaagag ccggccctcc ccgcgacgct ggacacttcg gaggcccgcc tgtcggcggc
     4021 ggcgtgcctg aggaggaagc gcgcggaggg acgacccgac caggctgggg aacaccgtga
     4081 cgtcccaggg cagctctagg cgtctctgcc accaggccag gcccaggggt cccaacaccc
     4141 gcaaggtcat tcgtccaccc ttgccgcgtc cgcggctcac cggccgcatc agcagctcct
     4201 cggtcgtgct aatggcgggc gcgagcgcaa gacgacgcac cccggtagcg agaccgagcg
     4261 gcgccggcag cgtttcctgc acgaggatgg tgagccgccg tctcttggca tggcgccagc
     4321 gatagcgcac gggaaagctc cgcccaactg aaaacgcggg cggcgcgttg cgttcgacct
     4381 ggagaaggaa cgaatccgct gcagcaacgg catcgaacgc gagcgcggca acccagagag
     4441 cgtccagaaa caggagaaga cctaacgcgc caggccagat caggcctgcc aatccgataa
     4501 gagcgagaca cgctccgacc gcaagccacc gttgtgaagg gattgcgatc accggggcgc
     4561 ttctacctgc tccagaatcg ccgcgagcgc cgcatcgggg gtgacgccct ccaattcgag
     4621 ctcgggggcg acgctgatcc ggtgtcgcag caccgctgga gcaacgctct tcacatcatc
     4681 cgggacgacg taatcgcgcc cgtcgagcag agcggtcgct tgagccagct tgagaagggc
     4741 gacgctgcct cggggggatg ctcccagcgt aagcgaggga gtcgtgcgcg tcgcccgaac
     4801 tatcgtggtc acgtaccgga ccagcgaagc ttccacccga acctgcctcg ctgccgcccg
     4861 gagatcggcc aggccgacac cgcccacgcc gggctcgatc ccatagctcc cgggatcgtc
     4921 cgcttcgaac ccactcagca cccgatggag cactccctgc tccgtctccg cagcgggata
     4981 gccgaggacg atcttgacca ggaatcgatc gagctcggcc tcgggcaatg gataggtgcc
     5041 ttcgaactcg atcgggttct gagtagcgaa gactgtgaat gccggggaaa gcgggtgcga
     5101 caccccgtcc accgtgacac tccgctcctg catggcttcg aggagcgctg cctgagtctt
     5161 ggcgggcgca cgattgatct cgtcaccaag aatcaggtcg ccgaagatcg gaccggcgcg
     5221 aaaggaaaac gtccccagcg cagtgagctg attgacgccg gtgatgtcgg aaggcatgag
     5281 gtccggcgtg aactgaatgc gacggaattc gagtccaagg ctgaggctca gacttcgaac
     5341 cagcagggtc ttcgccgttc ccgggactcc ctccagcaga acatggccac gagcgatgag
     5401 cgcggccacc gcttcacgaa tgaccggctc ctgacccagg acgacgcccc ccaacgccgc
     5461 aaccagatcg ttcgcggctt tgcagtgccg tccgatgtcg ccaaccgttc ctatggcttc
     5521 aggtcctccc atacctcctc caccacgttg gctgcctgca gcacgccttc cccgctctga
     5581 ggatgggtcg taaggtcgct gagagaagtc agggcctccc gcgcgcgggg cgacctggct
     5641 ctgcgggtca actcgcgcac ccagggacgc ggatcggcgc gaagcccggc ttctgcagcg
     5701 tgtggatggc gcgagagacg gcgccgcaga ccctgcacca tcaattgaat ggcgaggtcg
     5761 tgcccctcgg cggccgacag aacagtggcc agggcgcgga cgtgctccag cgaggaacgc
     5821 cgccggcgtt cgggcgaggg tcgcaccgga ccgaatcgta ccgcagcagc cgccaatgcc
     5881 accaaaccga tcccggcaag ctgccagccg gcccagcccc acggcgaccg agtgctccag
     5941 cccagcacgg agccggcaag cgagcccgag ggtccgtagc catgatgata ctcgtcgaag
     6001 atgatccgat cgtaacgccc cgccacgagg cgtagtgaga acggacccgc gcccgaggat
     6061 cgtagcgccc gattcgaaaa aggaaggtca tcagccacca gcgtgacggc tcgacctccg
     6121 acgatctcca gacgcagggc aaccacccga cctccgacag ttcggagcag ggtgtcagcc
     6181 cgcagggtgg tcggcgtatc gcagacagcg tagccctcgt cgtcccaggc agtgggccgg
     6241 cggcgcggac ggggctgagc ccgcgcgagc acagccccga cgcggaggtc gctcgcagac
     6301 attggctgac ccggggttat ggccagcatg ggctggagcc gcgtctcgac tacgtagcca
     6361 tagcatcgca tggcggcttc cgcgccctca ccggcgagaa gcaggtcgtg gcgagtaccg
     6421 aatcgggcaa tggccgccgc ctcgagcgga tcgatctcct cggtcggacc gaccaccgcg
     6481 agcacgggat tgccggtgtc cgaca
//
LOCUS       DASRJQ010000245         2226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_903818, whole genome shotgun sequence.
ACCESSION   DASRJQ010000245 DASRJQ010000000
VERSION     DASRJQ010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903818"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1128)
                     /locus_tag="VFB89_05560"
     CDS             complement(139..1128)
                     /locus_tag="VFB89_05560"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZM26800.1"
                     /translation="MDRRAFLAGAGALLAAPLAAEAQQQPGQVPRVGYISPGSSSDPF
                     RQRRFEAFRQGLRELGHVDGRNVVLEPRWAEGQYARYPVLAADLVRLKVAAIVTVGGA
                     ATKAAKQVTSTIPIVMSVVIDPIGSGLVTNLARPSGNLTGTSVMANELLTKQLELLKE
                     VAPNVHRIAVLWNPDNPSGAAAMAYLNAAARSLGVELQLVEARNPQEIDRAFAAMTRE
                     HAGAVVVLPDAIYGNQIKQIVGLAAQRRLPAIYTLVESAEAGGLMVYGPNLTDLERHA
                     ATFVNKILKGAKPADLPVEQPTKFDLVLNLKTAKVLGLTIPPSLLARADQVIE"
     gene            complement(1128..2105)
                     /locus_tag="VFB89_05565"
     CDS             complement(1128..2105)
                     /locus_tag="VFB89_05565"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZM26801.1"
                     /translation="MDRRRFLAGSAALLAAPVAAEAQQAVKVPRIGVLAPTSGTAGPA
                     AAFYRGLRDLGWIEGRNVAIERRSAENRVERFPSLAAELVQLRVDVILAAGGPASLTA
                     AKEATTTIPIVMVASSRDPVTDGLVQSFARPGGNITGLTTAPEEIGGKQLELLKEADP
                     RTSRVGILWDATVGAFRPSSSVEAVARSLKIELLPFEVHAPGDFEAAVAAARKERVNG
                     LMVAGTPMFTQHSKPLADLLARNRLPGISIWSTFPEAGGFMAYGPSLADEFRRAAIYV
                     DKILKGAKPGDLPIEQPTKYNLAINFKTAKALGLTIPPSLLARADQVIE"
BASE COUNT          383 a          723 c          729 g          391 t
ORIGIN      
        1 agccagaaca acatgccgtc cgtgcgtccc ttgaatggat actcgcggtc gatgcgatac
       61 actccggcct gctgcgcctc cgttgcgagc ggagggcaag cagcgcggca gcgcccgaaa
      121 ggaatacccg ccgcttcatc actcgatcac ctggtccgcg cgcgcgagta gcgatggcgg
      181 gatggtcagg cccaggacct tggcggtctt gaggttgagg acgagatcga atttcgtcgg
      241 ctgctcgacc ggcagatcgg cgggcttggc ccccttgagg atcttgttta cgaacgtggc
      301 ggcgtgccgt tccaggtcag tgaggtttgg accgtatacc atgagaccgc cggcctccgc
      361 gctctcgacg agcgtgtaga tcgcgggcag gcgtcgctgc gcagcgagcc cgacgatctg
      421 cttgatctgg ttgccgtaaa tcgcgtccgg gagaaccacg accgcccctg cgtgctctct
      481 ggtcattgcg gcgaaggcgc ggtcgatctc ctggggattc cgcgcctcca cgagttgaag
      541 ctctacgccc agcgagcgcg ccgccgcgtt caggtaggcc atcgctgccg caccgcttgg
      601 attatccgga ttccagagca cagcgatgcg atggacgttg ggcgccacct ccttgagcag
      661 ctccaactgc ttcgtgagca gctcgttggc catgaccgaa gttcccgtga ggttgccgct
      721 cggacgcgcg agattggtga ccagtccgct tccgatgggg tcgatcacga cggacatgac
      781 gatcgggatc gtgctggtga cttgcttggc ggccttggtc gcggccccgc ccaccgtcac
      841 gatcgcggct accttcagcc ggaccagatc agcagcaagg acgggatacc gcgcgtactg
      901 accttcggcc cagcgaggct ctagcacgac gttccgacca tcgacatgtc ccaactcgcg
      961 tagcccctgg cggaaggcct cgaatcggcg ctgccgaaac gggtcagagc tggagccggg
     1021 cgagatgtag cccacccgcg gcacttgtcc aggctgctgc tgcgcctcgg cggcgagggg
     1081 cgcggcgagg agggcgccag caccggcaag gaacgcccgc cgatccatca ctcgatcacc
     1141 tgatccgccc gcgccagcag cgacggcggg atcgtcaggc cgagcgcctt ggcggtcttg
     1201 aagttgatgg cgaggttgta cttggtgggc tgctctatcg ggagatcgcc gggtttcgct
     1261 cccttcagga tcttgtcaac gtagatcgcg gcacgacgga actcgtccgc gaggctgggt
     1321 ccgtatgcca tgaagccccc ggcctccggg aaagtactcc agatcgagat ccccggcagt
     1381 cgattcctcg cgaggaggtc ggcgagcggc ttcgaatgct gcgtgaacat cggagtgccg
     1441 gcgaccatca gaccattgac ccgctccttt cgcgcagcag caactgctgc ttcgaaatcc
     1501 cccggggcgt gtacttcaaa cgggagaagc tcgatcttca gcgaccgagc caccgcctcc
     1561 actgagctgg acggccggaa ggcgccgacg gtcgcatccc ataggatgcc gacccgcgac
     1621 gttctcggat cagcttcctt caacaactcc agctgttttc ctccgatttc ttctggagcg
     1681 gtcgtcaggc ccgtgatgtt gccgcccggg cgcgcgaagc tctggacgag cccatcggtg
     1741 actggatcac gggatgaggc caccatcacg atggggatcg tcgtcgtcgc ctccttggcg
     1801 gcggtgaggg atgccgggcc tcccgccgcg aggatgacat cgacacgcag ctggacgagt
     1861 tccgccgcca aactcgggaa tcgctcgacc ctgttctctg cagatcggcg ctcgatggcg
     1921 acattccgac cctcgatcca gccaaggtcg cgcaggcccc ggtagaaagc cgcagcgggg
     1981 ccggcagtac ccgacgttgg tgccagaact ccgattcggg ggactttcac tgcctgctgc
     2041 gcctcggcgg cgaccggtgc ggcgagcaac gcggcagaac cagcaaggaa tcggcggcga
     2101 tccatcactc gatcacctga tcggcctgca gcaggagcga ggacgggatg gtgaggccga
     2161 cggctttagc ggtcattcgt cgcgtcgcac cgggaccgtg aacgtgaagg tcgagcccac
     2221 gtccac
//
LOCUS       DASRJQ010000246         3571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2602016, whole genome shotgun sequence.
ACCESSION   DASRJQ010000246 DASRJQ010000000
VERSION     DASRJQ010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2602016"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="VFB89_05570"
     CDS             complement(<1..727)
                     /locus_tag="VFB89_05570"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZM26802.1"
                     /translation="MPDSTDVVVVGGGAIGAACARELARSGRRVRILDPEADRGQAWR
                     AAAGMLAPQIEAREGDALFELGLAGRELYADLVPTLEESTGIKIGLWRDGIANVALRE
                     ADVGGLRAKVAWQRQQGHLCDWLDASEVKERWPWIGPNFGALWAAREGALDPQGLVEA
                     LRADAVRHGTELIRDEAVGLETRGDRVVAVEAKGRRYPTEHVVIAAGAWSGNLAGLPV
                     PLAVAPIRGQMLALPWPETVSRAI"
     gene            802..1017
                     /locus_tag="VFB89_05575"
     CDS             802..1017
                     /locus_tag="VFB89_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26803.1"
                     /translation="MLRNLVGFAVFAIVAILAFKLLVGLFGVMVGLLMSLLWLAFVGW
                     LIYLVIRVFSPATADRIREVIRGRQAA"
     gene            complement(1081..1899)
                     /locus_tag="VFB89_05580"
     CDS             complement(1081..1899)
                     /locus_tag="VFB89_05580"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZM26804.1"
                     /translation="MIYVCIPCYNEAPTVGLLLWKIRQVFSGFPREYHLLLIDDGSTD
                     ATAEVLEPYTRVLPLTMLRHESRLGYAASVEALLRLAVQRTDRPKRDGAILMHADFAH
                     SPSFIPDFLRRLESGADIVVAQSTVTGESSRARRLLRRFAPLLLRGRVRVPGVADVVS
                     GFLAFRLVVLKNAIRGQEGPLLTSDGWTANAELIGKTARHARRVDTIPAVERHDLRQR
                     PSRIEPWDTALSVWQGRWKLRLPARQTAEDRSSGHRAEPTAAEAEPEDETVKSP"
     gene            complement(1931..>3571)
                     /locus_tag="VFB89_05585"
     CDS             complement(1931..>3571)
                     /locus_tag="VFB89_05585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZM26805.1"
                     /translation="GGTLTPEDAAFDWILVPLVTGAEPTGALAIGREPGSFSSSDLAT
                     AQALAAFACLTLQRIASAGVELPRPSWQEGLDAIAPALCLIDQTECIRRANRAFAWLV
                     TTPPDGLIGRPWQSLVPADWVPHLERAIRAEGISREVELRLGDRVYSASVAPLPGPSG
                     ADVVLVFDDQSDRHRLQDQLVQSEKMSAIGQLIAGVAHDLNNPLASVVGFAEFLSERP
                     DVPPALREPVTVIREEAERASGIVKNLLSFARKQERRRRPTAMQPLLESTVALLRNEL
                     MSTKVECRIEVDPDLSTPVIDANQIQQVFVNLIHNAAQAIAAGGRSGTITVRARRWTD
                     GIAVDVIDDGPGMSEALATQVFDPFFTTKPEGHGTGLGLSISQGIVTEHGGRITLETA
                     EGWGSTFTVHLPAGEGAAPVVTEGTPPAAPGLRILVVDDEPHILHYMRATLESWGHSV
                     SQAADGEEARERLSGDGFDLVIADLRMPRLGGREFYEELKRTRPAQAEQVVFATGDTV
                     RGDTLAFLKSVGRPYLHKPFSLTELRALLAGVSKVPDE"
BASE COUNT          633 a         1147 c         1143 g          648 t
ORIGIN      
        1 ggattgctcg gctaaccgtc tcgggccagg gtaaggccag catctgcccc cgaatcggtg
       61 caaccgccaa cggaacgggc aggccggcga gattgccgct ccacgcgccc gcggcaatga
      121 caacatgctc cgtaggatag cgtcggcctt ttgcttcgac cgccaccacc cggtcgcccc
      181 gagtctcgag cccgacggct tcgtcgcgga tcagctcggt cccgtgcctc acggcatcag
      241 cgcgaagtgc ctcgaccagg ccctgaggat cgagcgctcc ctcgcgagcg gcccaaagtg
      301 cgccaaagtt tggaccgatc cagggccatc gttctttcac ctccgaggca tccagccagt
      361 cgcagaggtg gccctgttgt cgttgccagg ctaccttcgc tcgaagaccg ccaacgtcgg
      421 cttcgcgcag cgcgacattg gcaattccat ctctccagag tccgatcttg atgccagtcg
      481 actcttccaa ggtgggaacc agatccgcat agagttcgcg ccccgcgagc cccagctcga
      541 aaagagcgtc accttcgcga gcttcgattt ggggagccag catgcccgcg gcggcgcgcc
      601 aggcctgccc tcggtcggcc tcggggtcaa ggatgcgcac acggcgtccg gatcgggcga
      661 gctcgcgggc gcaagcggcg ccgatggcgc cgccacccac gacaacaacg tcagtggaat
      721 cgggcactta ggtggtctat gaaacttttt gaaacgtgcg ggcgtacggc cggctgactc
      781 gcgcctcgga ggataatacc aatgctgcgg aacctggtcg gattcgccgt cttcgccata
      841 gtggcgattc tggcgttcaa gcttctcgtc ggtttgttcg gtgtgatggt cgggctgctc
      901 atgagccttt tgtggttggc gttcgtcggc tggctgattt atctggtgat cagagtgttc
      961 agtccagcga cggcggaccg gattcgagaa gtcatccgag gaagacaagc ggcgtaagcg
     1021 gtccgaggcg ctgtcagggc gtgcaagggc tccgcgtttt aaagcggagc cctttgcctc
     1081 ctatgggctc tttaccgttt cgtcctcggg ctcggcctcc gctgcagtcg gttcggcccg
     1141 gtggccgctc gaccgatctt ccgccgtttg ccgggcgggt agccgcagct tccagcgccc
     1201 ttgccacacc gacagtgccg tgtcccacgg ctcgatccgg ctcggccgtt gacggagatc
     1261 atggcgctcg acggctggaa tggtgtctac ccgccgcgca tgccgcgcgg tctttcctat
     1321 caattcggca ttggccgtcc agccgtccga agtcaacagg gggccttcct gaccgcggat
     1381 cgcattcttg agaaccacca gtcggaacgc caggaacccg gacacaacgt ccgccactcc
     1441 cggtacccgc acccgtcccc ggagaagcaa gggggcaaac cgccgcagca gtcggcgggc
     1501 acgggagctt tcgccggtga ccgtactctg ggccacgaca atgtcagcgc cgctctccag
     1561 ccgcctcagg aaatccggga tgaagcttgg gctgtgggca aagtccgcgt gcatcagaat
     1621 ggcgccgtcc cgcttcggtc gatccgtgcg ctgaacggcg agcctgagca gcgcctcgac
     1681 cgaggcggcg tagccgaggc gactttcatg acgcagcatg gtcagaggca gcactcgggt
     1741 atacggctcc agcacctcgg ctgtggcatc ggtcgagccg tcgtcgatca gcagcaggtg
     1801 atattccctc gggaaaccgc tgaagacctg gcggatcttc cagaggagaa gcccgacggt
     1861 cggggcttcg ttgtagcagg gaatgcagac gtagatcatg aaacgcgggg tgcggaccgg
     1921 cggaaggata tcactcgtcc ggcaccttgg acacccccgc gagcaacgct cgcagctcgg
     1981 tcaggctgaa gggcttgtgc aggtaggggc gaccgaccga tttgagaaaa gcgagggtgt
     2041 cgccgcggac cgtgtcaccg gtggcaaaca cgacctgctc ggcctgggcc ggtcgggtcc
     2101 gtttcagctc ctcgtaaaac tcccggccgc caaggcgcgg cattctgaga tccgcgatca
     2161 ccagatcgaa gccgtctccc gagagacgct cgcgcgcctc ttccccgtcc gcagcctgcg
     2221 acactgagtg cccccagctc tcgagagtgg ctctcatgta gtggaggatg tgcggctcgt
     2281 catccaccac cagaatacgc agtcctggcg ccgcgggtgg cgttccctcg gttacgacag
     2341 gagcagcgcc ctctcccgcc ggaagatgaa ccgtgaacgt tgagccccaa ccctcggcgg
     2401 tttccagggt gattcggccg ccgtgctcag tcactatgcc ttgtgaaatg gaaagcccca
     2461 acccggtccc atgcccttcg ggcttggtgg tgaagaaggg atcgaacacc tgagtcgcca
     2521 gcgcctcgga cattccgggg ccgtcgtcga tcacgtccac ggcgattccg tcggtccacc
     2581 gtcgcgcgcg aaccgtgatg gtgccggagc gtccgccagc ggcgatggct tgcgccgcgt
     2641 tgtgaatgag attgacgaag acttgctgga tctgattggc gtcgatgacg ggagtcgaca
     2701 ggtccgggtc gacctcgatt cggcattcca ccttggtcga catcagctcg ttccgcagca
     2761 atgcaaccgt ggattcgagc agcggttgca tagcggttgg gcgacggcga cgttcttgtt
     2821 ttcgggcaaa gctcaggaga ttcttcacga taccggaagc gcgctcggct tcctctcgaa
     2881 tgaccgtcac cggctcgcgc agggctggcg gaacatcggg ccgctccgac agaaactccg
     2941 caaagcccac gactgaagcc agcgggttgt tgaggtcgtg ggccacgccc gcaatcagct
     3001 gaccgatggc cgacattttt tccgattgca ccaactggtc ctgaagtcgg tggcggtccg
     3061 actgatcatc gaacaccagt accacgtccg cgccactcgg gccgggtaac ggtgccaccg
     3121 acgccgaata cacccgatca ccgaggcgaa gctcgacctc gcgactgatt ccttccgctc
     3181 ggatcgcacg ttcgaggtgc ggcacccagt ccgccggaac cagagactgc catgggcgac
     3241 cgatcagtcc gtctggtggt gtggttacga gccaggcgaa ggcgcgattg gcacggcgta
     3301 tgcactcggt ttggtcgatc aggcacaagg ctggggcaat cgcatcgagg ccttcctgcc
     3361 acgaaggacg cggcaattca actccggctg aggcaattcg ctggagcgtg aggcaggcaa
     3421 aggcagccag cgcctgtgca gttgcgagat cactggaact gaagctgccg ggttcgcgtc
     3481 cgatggccaa cgcgcctgtt ggctcggcgc ccgtgacgag cggcacaaga atccagtcga
     3541 atgcggcgtc ctccggtgtg agcgtgccgc c
//
LOCUS       DASRJQ010000247         3799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1030271, whole genome shotgun sequence.
ACCESSION   DASRJQ010000247 DASRJQ010000000
VERSION     DASRJQ010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030271"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VFB89_05590"
     CDS             <1..303
                     /locus_tag="VFB89_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZM26806.1"
                     /translation="GLGDVPGVLRFGPAPGLPRTPTVSFVLEGRLSEQVARACAARGV
                     FVSNGDFYATTVVERLGYAADGLVRAGCACYTTTEEVDRLIGCVRLAAAGAGLAAG"
     gene            complement(334..918)
                     /locus_tag="VFB89_05595"
     CDS             complement(334..918)
                     /locus_tag="VFB89_05595"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HZM26807.1"
                     /translation="MSILLTLSLAAAPGVSLPPGARPADPACITMNTKNLPLATRKSP
                     LDSVSFTAAGSQVKVCYGRPSLRGRHMIGSDAVPYGKIWRTGANEPTMIHTTGPLSIA
                     GVKVGAGTYSLYTVPGPGQWEVIVNRSITQWGEESNYTDAVKKQEVGRGKVSAETLPQ
                     SVETFTIKPDPSSGEAKALLLEWEKAKVTIPLAK"
     gene            complement(943..1434)
                     /gene="purE"
                     /locus_tag="VFB89_05600"
     CDS             complement(943..1434)
                     /gene="purE"
                     /locus_tag="VFB89_05600"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HZM26808.1"
                     /translation="MQPLIGIIMGSDSDWPVMQHAAKRLKDFGVPWEARVLSAHRTPD
                     LAVAYAEEASKRGIRCLIAGAGGAAHLAGVLAAKTTLPVLGVPMPSKHLQGLDSLLST
                     VQMPGGIPVATFAIGEAGAANAALFAVAMLAGTDETLARKLVDFRHAQADKIQKITLP
                     DPA"
     gene            complement(1458..>3799)
                     /gene="ppdK"
                     /locus_tag="VFB89_05605"
     CDS             complement(1458..>3799)
                     /gene="ppdK"
                     /locus_tag="VFB89_05605"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HZM26809.1"
                     /translation="MMETILNLGLNDTTVEGLAALSGNPAFAWDSYRRFVQMYGAVVF
                     ELSKQPFEHKLDARKRECGVARDIDLPVPVIQALVREIKELVRQQAGKPFPDDPVAQL
                     WGAIAAVFSSWNTRRAIDYRKLHGVPDTMGTAVNVVAMVFGNLGEDSGTGVAFTRDPS
                     TGEPRLYGEYLLNAQGEDVVSGARTPEPITSLEGRMPHAYQELERTARVLERHFRDVQ
                     DLEFTIERGRLYMLQTRRAQRSGPAAVRIACDMVEERLISQDEAVARIPPRDLDQLLH
                     PTIDQAGKLDLLAVGLPASPGAACGTVVFDADRAERLGRSGQPVILVRRETSPEDFHG
                     MVAAKAVLTARGGMTSHAAVVARGMGKPCVAGASRIDVDEPNHRFSADGRVISEGDWI
                     SLDGGTGKVFAGQARLIEPSLGEDFKQLMGWADGVRQLRVRVNADTPADAHRGRDFGA
                     EGIGLCRTEHMFFEGDRLVVMREMILAQDEAGRRRALDRLLPMQRGDFEAIFRAMEGF
                     PVTIRLLDPPLHEFLPKEEAEIDALARQLGRAVEDIRRAVQSLQEVNPMLGLRGCRLG
                     IVHPEITQMQTRAIFEAACAVAARRGLVLPEIMIPLTASVVEFRKQALVVRRVAEDVF
                     NERQITVPYLLGTMIELPRAALTADEFALEAQFFSFGTNDLTQTTWGLSRDDAGRFLP
                     QYIELGIVDGDPFQELDLAGVGKLIQMACELGRKTRADLKLGICGEHGGEPNAVAFCD
                     RLGVNYVSCSPFRVPIARLAAAQAALAERGTMDRTKATV"
BASE COUNT          638 a         1226 c         1263 g          672 t
ORIGIN      
        1 ggtcttggcg acgtccccgg tgtgctcaga ttcgggcctg caccgggact ccctcgcacg
       61 cccacggtct ccttcgtgct cgagggacgg ctatcggaac aggtggccag ggcctgcgcc
      121 gctcgggggg ttttcgtctc caacggagac ttctacgcca cgacggtggt cgagcgactg
      181 ggctatgcgg ctgacgggct ggtcagggca ggctgtgctt gctacaccac tacggaggaa
      241 gtcgaccggt tgatcggctg cgttcgcctc gcagctgcgg gggccggcct ggccgcgggc
      301 tgaagaaccc gcggtccagg cggcgaacag cgactacttg gcgaggggga tggtgacttt
      361 cgctttctcc cattccagca gcagagcctt ggcttcgccg ctcgaggggt ccggcttgat
      421 ggtgaacgtt tccacggact ggggaagcgt ttctgcgctc accttgcccc gtccgacctc
      481 ctgcttcttg acggcatcgg tgtagttcga ctcttcgccc cactgagtga tcgagcggtt
      541 aacgatcacc tcccactgac ccggacccgg aacggtgtag agcgagtagg ttcccgcgcc
      601 gactttgacg ccggcaatcg aaaggggacc ggtcgtgtgg atcatggtgg gctcatttgc
      661 tcccgtacgc cagatcttgc catagggcac agcatcactg ccgatcatgt gtcggccgcg
      721 aagggacgga cgcccatagc agaccttgac ctgacttccc gcagccgtga acgagaccga
      781 gtcgagcggg ctcttacggg tggcgagcgg caggttcttg gtgttcatcg tgatgcaggc
      841 gggatcggcc ggccgtgctc cgggtggcag ggacactccc ggggctgcgg cgagtgaaag
      901 ggtcaagaga atcgacatgg gactgaggtt cctggctttg agtcaagcgg gatcgggcaa
      961 ggtgatcttc tgaatcttgt ctgcctgggc gtggcggaaa tccaccagct tgcgagccaa
     1021 cgtttcatct gtcccggcga gcattgcgac ggcgaagagg gcagcgttgg ccgccccagc
     1081 ctcgccgatc gcgaatgtcg cgaccggaat cccacccggc atctgcacgg tcgacagcag
     1141 ggaatcgagc ccctggaggt gcttgctcgg catggggacg cccagcaccg gcagtgtggt
     1201 cttggccgca agcacgccgg ccaaatgtgc cgctcctccg gctcctgcaa tcagacagcg
     1261 aatgccgcgc ttgctcgcct cctcggcata ggccacagcg agatcagggg tgcgatgggc
     1321 cgagagcacg cgggcctccc aaggcacacc gaaatccttg agtcgcttgg ccgcgtgctg
     1381 catgaccggc cagtccgagt cgctgcccat gatgatgccg atgaggggtt gcaagcttgg
     1441 ctcctgtgat gggcgggcta gaccgtcgcc tttgtccgat ccattgtccc ccgttccgcc
     1501 aacgcggcct gcgccgctgc aagacgggcg atcggaaccc ggaatggcga acaggaaacg
     1561 tagttcacac ccaaccggtc gcagaatgcc actgcgttgg gctcgccgcc gtgctcaccg
     1621 cagatgccga gcttgaggtc ggcccgggtc ttccgcccca gctcgcaggc catctggatc
     1681 agcttgccca cgccggcaag gtcgagctcc tggaagggat cgccgtccac gatgcccagc
     1741 tcgatgtact gcggcagaaa tcgcccggcg tcatctcgcg agaggcccca cgtggtctgg
     1801 gtaaggtcgt tggtcccgaa ggaaaagaac tgggcttcca gcgcgaactc gtcggcagtg
     1861 agcgcagcgc ggggcagctc gatcatggtg ccgagcagat agggcacggt gatctgacgc
     1921 tcgttgaata cgtcttctgc gacccgccgc acgaccaggg cctgcttgcg aaactcgaca
     1981 accgatgcgg ttagcggaat catgatctcg ggcagcacca ggccgcgtcg ggcggcgacg
     2041 gcgcaggccg cctcgaagat cgcccgggtc tgcatctggg tgatctcggg gtgcacgatg
     2101 ccgagccgac acccccggag ccccagcatg ggattgactt cctgcaagga ttgcacggcc
     2161 cggcggatgt cttcgactgc gcgaccgagc tggcgggcga gcgcgtcgat ctctgcctct
     2221 tccttgggca ggaattcgtg gagtgggggg tcgagcagac ggatggtaac gggaaatcct
     2281 tccatcgccc ggaagatggc ttcgaagtct cctcgctgca tcggcaggag gcgatccagc
     2341 gctcggcggc ggcccgcctc gtcctgcgcc aggatcattt cccgcatgac gaccaggcgg
     2401 tcgccctcga agaacatgtg ctcggtgcga cagagcccga taccttccgc gccgaaatcg
     2461 cggcctcgat gggcgtcggc aggggtgtca gcgttgaccc ggacccggag ttgtcgcacg
     2521 ccgtcggccc agcccatcag ttgcttgaag tcttcgccca aactgggctc gatcaggcgg
     2581 gcctggccgg cgaagacctt accggtgcct ccgtcgaggc tgatccagtc gccttcgctg
     2641 attacgcggc cgtcggcgga gaaccggtgg ttaggctcgt ctacgtcaat cctcgacgca
     2701 ccggccacgc agggcttacc catcccgcgg gcgactacgg ccgcgtgcga ggtcatgccg
     2761 ccgcgcgcgg tcagaaccgc cttcgccgcc accatgccgt gaaagtcttc gggcgaagtc
     2821 tcgcgccgga ccaggatcac cggctggccg ctccgcccga gccgttcggc tcgatcggcg
     2881 tcgaacacca cggtgccgca cgcggccccc ggcgaggcag gcaatccgac ggccaggagg
     2941 tcaagcttgc cggcctggtc gatagtggga tggagcagtt gatcgagatc tcgcggcgga
     3001 atccgcgcga cggcttcgtc ctgtgagatc aggcgctcct ccaccatgtc gcaggcgatc
     3061 cgtactgcag cggggccgga gcgctgggcc cggcgggtct gcaacatgta gagtcggccg
     3121 cgctcgatgg tgaactccag atcctgcacg tcccggaaat gccgctccag aacgcgagcg
     3181 gtacgctcga gctcctggta ggcatgaggc attcgcccct cgagggaggt aatcggctcg
     3241 ggcgtgcgcg cgcccgacac gacgtcctcg ccctgcgcat tcaggaggta ttccccgtac
     3301 agccgcggtt cgccggtgga gggatcacgg gtgaacgcga cgccggtgcc cgaatcttca
     3361 cccagattcc caaacaccat ggcaacgacg ttcaccgctg tgcccatcgt gtccggcacg
     3421 ccgtggagct tccgatagtc gatggcgcgg cgggtattcc agctcgagaa caccgcagca
     3481 attgctcccc agagctgggc cacgggatcg tcgggaaacg gctttccggc ctgctgtctg
     3541 accagttcct tgatttcacg caccagggcc tggatgaccg gcaccgggag atcgatgtcg
     3601 cgggcaacgc cgcactcgcg cttgcgggca tccagcttgt gctcgaacgg ctgcttggac
     3661 agctcgaata ccacggcccc gtacatctgc acgaatcgcc ggtaggaatc ccaggcaaac
     3721 gccggattgc ccgacagggc tgcgagtcct tcgaccgtgg tgtcgttcag gccgaggttg
     3781 aggatcgtct ccatcatcc
//
LOCUS       DASRJQ010000248         7749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2257733, whole genome shotgun sequence.
ACCESSION   DASRJQ010000248 DASRJQ010000000
VERSION     DASRJQ010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7749
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2257733"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VFB89_05610"
     CDS             complement(<1..393)
                     /locus_tag="VFB89_05610"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HZM26810.1"
                     /translation="MTSAAFVPVTRWTDRRHLRGVRGELHALAYLVSCGWEIESHRFR
                     LGRNDIDLVVRRGCLIAFVEVKTRKSSKCGAPGEAVSARKQATLARVAAGWRARYGRP
                     GDAYRFDLVSVWVEGREPVRVEHVADAWR"
     gene            complement(494..985)
                     /locus_tag="VFB89_05615"
     CDS             complement(494..985)
                     /locus_tag="VFB89_05615"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HZM26811.1"
                     /translation="MVLNAGRVQQVAPPHTLYEKPANTFVASFIGSPAMNLLQGELLE
                     GPSPTFRAAGGGFTLRTPSVGRDVLGGRSDVPVVLGVRPEEIRLQGSVSDAAGSVVRC
                     RVDLVELLGGEVLIHASNNGHELTARTGTEATNQPGDEISLVIPPASVHLFDASTGQR
                     LSA"
     gene            complement(976..1608)
                     /locus_tag="VFB89_05620"
     CDS             complement(976..1608)
                     /locus_tag="VFB89_05620"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZM26812.1"
                     /translation="MASVTLDGVAKTYTERGGTHTAVHRVDLDIRDGEFVVLVGPSGC
                     GKTTTLRMIAGLESISEGELRIGGRVVTHLAPKERDVAMVFQNYALYPHMSVYDNLSF
                     ALRLRHVEQPEIDRRVREAAELLGLARLLDRKPRQLSGGERQRVALGRALVRKPQVFL
                     FDEPLSNLDAKLRVHMRREIARLHRELGATMIYVTHDQVEAMTLWSESWC"
     gene            complement(1612..3894)
                     /locus_tag="VFB89_05625"
     CDS             complement(1612..3894)
                     /locus_tag="VFB89_05625"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HZM26813.1"
                     /translation="MSTDSRHRLGAITALVLGVFVGLTLLPFELTGPVGRWVGHELWH
                     ALGIGATGLPLLGLGLALAGFERLGRLDMKRAAVLMIGLSLLVPYLVGVVAKVTHLDL
                     VPDVDQRALPARLAGLVPGMLAEVVAGRVGVAGAVLLGFLALSALTLVTFAWHPLQRL
                     EAGRSGKQPDRATGTRAEEEKAAKRGASKTPSLPPVTMDTPDPVQNGVGKSGAAARGA
                     GEPAKKARGKTPSVKRGEGVSEALGPVWEVELLDPPSGKAIDAGESELDSLQERLEAT
                     LAEFKVEGDVAGRTTGPVVTQYGVRLRPGVKMNRLVALADDLALKMSARSIRVARIPG
                     RDMVGVEIPNPKSRVVMLRELLEDDAWQGEDRLLPVALGLDLEGRPVIADLGKMPHLL
                     IAGATGTGKSIGINSIITSLIYQYQHKEELRFLMIDPKMVELSMYKDLPHLRHPVVTN
                     NKEAARVLKWAVNEMERRYALLEANGVRNVTDFNRKLLDGKPLRNPSPKKATLSEISA
                     EISSTPPPPPLEDLYTQGKLPLIIVVVDELADLMMTVQAEVEAPLARLAQKARAVGLH
                     LIVATQRPSVNVITGLIKANFPSRIAFRVAQKVDSRTILDQNGAEALLGKGDMLFLEP
                     GKSEPMRLQGAYISTEESQRLMDRYRDWKAAQDKRGVADGVETNILDEVPEAEAGERG
                     DEQFDVTERDPLFKEAALACVQNQGGSTSLLQRKLGIGYGRAARLMDQLEEAGILGPQ
                     NGSKPRDVRIGIEQVEEYCS"
     gene            complement(3891..4661)
                     /locus_tag="VFB89_05630"
     CDS             complement(3891..4661)
                     /locus_tag="VFB89_05630"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HZM26814.1"
                     /translation="MKLHVWFSPLGLGLADVQGRTIFVIDVLRATTVMCAALHHGARA
                     LIPVASTEEALRLAQTIGSADVLLAGEKNCLRIPGFALGNSPLEMTEQTVKGKTIVVT
                     TTNGTKALLSTQGAAAVYVASAANLTLAGERAREALEENRDVVILCAGRETAFSLDDA
                     YCAGRLAVAALGDHKPRKGLNDAALASLDLVRRYGDDWTRPLSYSRAGRELIRLGFRD
                     DVLDAGRLDAYPVLPQFHERRVTVAPQSQPQAAQAQPS"
     gene            complement(4661..6016)
                     /gene="accC"
                     /locus_tag="VFB89_05635"
     CDS             complement(4661..6016)
                     /gene="accC"
                     /locus_tag="VFB89_05635"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HZM26815.1"
                     /translation="MFKKVLIANRGEIALRVIRACRELGVQTVAVYSEADRESLHVRF
                     ADDDVCIGPPPGRLSYLKIPNLIAAAEITGADAIHPGYGFLAENAEFADTCRASNITF
                     IGPTGEQIRLMGDKATARRLAQEAGVPTVPGSPGTIDDPDEAVRFAQEIGFPVIIKAT
                     AGGGGKGMRIAHDLEQFSELFGLAQNEALAAFGNGAVYVEKYLERPRHVEIQVLGDSH
                     GKVVHLGERDCSVQRRHQKLIEESPSPALDHALRRRMGESAVALAANIGYQGAGTLEF
                     LLDPDGKFYFMEMNTRIQVEHPVTEMVTSFDLVKEQIEVAAGAPISYRGDGTRLRGHA
                     IECRINAEDPYRNFQPSPGLITAYHPPGGPGVRVDTHVYAGYTVPPYYDSLLAKVIVH
                     GNTRQEALSRMGQALDSFILEGITTTIPFLARVIRHPDFAAGKVDTRFLERESHLLQP
                     D"
     gene            complement(6462..7637)
                     /locus_tag="VFB89_05640"
     CDS             complement(6462..7637)
                     /locus_tag="VFB89_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HZM26816.1"
                     /translation="MAEATAPGAVAAFNFKQAIAQMIQRNGSDLLLKVGRAPTVRVNG
                     ELTTLEMSALKPEDLKLLAEQVMTPRQVKEFAEKKEADFAIGVPGVGRFRTNIYQQRG
                     TLAFAFRAIPYEVKTIRELNLPAILEEISLKPRGIVLVTGITGSGKSTALASMIHHVN
                     QHRRCNVITIEDPIEFLHRDVQSNISQREVGSDTLSFGAALRHVLRQDPDVILIGEVR
                     DMETLDTALKAADTGHLVFSTLHTTDATQTINRIISFYPPYQHQEIRSLLSTALAAIV
                     CLRLVPRKDGRGRVPAAEVLINTAAVADNIRDIEKALNIPDLIAEGTVSYGMQSFDQS
                     LMKWYKEGMVAYESALFYSSNPSEFALRVSGVDSASDRTFSELSGDAAARKSLDLTP"
BASE COUNT         1336 a         2553 c         2448 g         1412 t
ORIGIN      
        1 ccgccatgca tctgccacat gctcgacccg aaccggctcc ctcccctcca cccagacact
       61 caccaggtcg aaccggtacg cgtcgcccgg ccgtccgtaa cgagcgcgcc agcccgcggc
      121 gactcgagcc agggtggcct gcttgcgcgc gctcaccgcc tcgcccggtg caccacactt
      181 gctgctcttt cgagtcttga cctcgacgaa ggcaatcagg caaccgcgcc gcaccaccag
      241 gtcgatatcg tttcggccca ggcggaatcg gtgggattcg atttcccacc cgcacgagac
      301 caggtacgcg agtgcgtgga gctcgccgcg cacgccgcgc aggtggcggc ggtcggtcca
      361 ccgggtaacc gggacgaagg ctgcgctcgt catggagcga gcatatcgga gcatcccgcc
      421 cgggcctgca tcgaacgcgc gcaaaccagc accgaatcgt gaggaatcct cgattggaac
      481 ttctgatcag gcatcaggcg gagagtcttt gaccggtgct ggcgtcgaag aggtgcacag
      541 aggcaggagg aatgacgagg gatatctcgt caccgggctg attggttgct tccgttccag
      601 tccgagcggt gagctcgtga ccgttgttgc tggcatgaat gaggacttcg ccgcccagca
      661 gctcgaccag atcgacccgg cagcgcacca ctgagccggc ggcgtccgaa acgctaccct
      721 gaagccgaat ctcctcgggt cggacaccca gcactaccgg cacgtcggaa cgtccgccca
      781 gcacgtcgcg cccgactgat ggcgtccgaa gtgtgaatcc cccgccggcg gcccgaaacg
      841 tgggggacgg accttccagc agctcgccct gaagcagatt cattgccgga cttccgatga
      901 agctcgcgac gaacgtgttt gccggcttct catacagcgt gtggggggga gccacctgct
      961 gaactcgccc ggcattcaac accacgattc gctccacagc gtcattgcct cgacctgatc
     1021 gtgggtcacg tagatcatgg tcgcacccag ctcacggtgc agccgggcaa tctcgcgtcg
     1081 catgtgcacc cgcagctttg cgtcgagatt ggacagcggt tcgtcgaaca ggaatacctg
     1141 cggcttgcgc accagggctc ggccaagggc gacccgttgt cgctcgccac cggagagctg
     1201 ccgtggcttg cggtccagca accgcgcgag acccagcagc tctgctgcct cccgtacccg
     1261 acgatcgatc tcgggctgct cgacatgccg aagccggagc gcgaacgaca ggttgtcgta
     1321 gaccgacatg tgcggataga gcgcgtaatt ctggaatacc atcgcgacgt cgcgctcttt
     1381 gggagctagg tgggtgacga cgcggccgcc gatccgaagc tctccctcag aaatcgattc
     1441 caggcccgcg atcatccgca atgtggtagt cttgccgcag ccggaaggac cgaccagaac
     1501 gacgaactcg ccgtcccgaa tgtcgagatc gacccggtgc acggcggtgt gggtaccgcc
     1561 ccgctctgtg tatgtcttgg ctacaccgtc cagcgtcacg cttgccatcg gtcagctaca
     1621 gtattcctcc acctgctcga tgccgatccg cacgtcccga ggcttgctcc cgttttgcgg
     1681 gccgagaatc ccggcttctt cgagctggtc catgagccgc gcagcccgcc cgtagccgat
     1741 cccgagcttc cgctgaagca gcgaggtgga accgccctgg ttctggacac aggccagtgc
     1801 ggcttccttg aacagcgggt cgcgctcggt tacgtcgaac tgctcgtccc cccgctctcc
     1861 cgcctccgcc tcggggacct cgtccaggat gttcgtctcc acaccatctg cgactccgcg
     1921 cttgtcctgc gccgccttcc agtcgcggta gcggtccatc agccgctgac tttcctccgt
     1981 cgagatatag gccccctgaa ggcgcatggg ctcgctcttg cccggctcaa ggaacagcat
     2041 atcccccttc ccaagcaacg cttcggctcc gttctgatcg agaatggtgc ggctatccac
     2101 cttttgtgcc acccggaatg cgatgcggct gggaaagttg gccttgatga gaccggtaat
     2161 gacgtttacc gacggtcgct gggtcgccac gatgagatgt aagcccacgg cgcgagcttt
     2221 ctgcgccagt cgcgcaagcg gcgcctccac ttcggcctga acagtcatca tgaggtcagc
     2281 cagctcgtcc accaccacaa tgatgagggg cagttttccc tgggtataca ggtcctccag
     2341 tggcggcggg ggaggtgtgc ttgagatctc ggcggagatt tcggagaggg tggctttctt
     2401 gggactcgga tttcgcagcg gcttgccgtc cagcagcttg cggttgaaat cggtgacgtt
     2461 ccgaaccccg ttcgcctcca acaatgcgta gcggcgctcc atctcgttaa ccgcccactt
     2521 gagcacccgc gcggcctctt tgttgttggt caccaccgga tgccgcagat ggggcagatc
     2581 cttgtacatc gacagctcga ccatcttggg atcgatcatc aggaagcgca gctcttcctt
     2641 atgctggtac tggtatatca gcgacgtgat gatcgaattg atgccgatcg acttgccggt
     2701 gccggtggcg ccagcaatca gaaggtgcgg catcttcccc agatcggcga tgaccgggcg
     2761 gccttcgaga tcgaggccga gggccacagg cagcaagcga tcctcgccct gccaggcgtc
     2821 gtcctccagc agctcccgta gcatgaccac gcgcgacttg ggattgggta tctcgacgcc
     2881 gaccatgtcc cgccccggaa tgcgcgcgac cctgatcgag cgagcgctca tcttgagcgc
     2941 gagatcgtcg gccagcgcga ccagccggtt catctttacc cccggacgca ggcgcacgcc
     3001 gtactgagtg accaccggac cggtggtacg tcccgccacg tcgccctcga ccttgaactc
     3061 cgccagcgtg gcctcgagcc gctcctgcag ggagtccaac tcgctttcgc ccgcgtcgat
     3121 cgccttcccg gacggtggat cgagcagctc gacttcccag actggcccga gtgcctcgct
     3181 gacaccctct ccacgcttga cgcttggggt tttgccgcgt gccttcttgg ccggctcacc
     3241 ggccccacgc gccgctgcac cggatttccc tactccgttc tggaccgggt cgggcgtgtc
     3301 catagtgacg gggggtagcg atggcgtctt cgaggcgcca cgcttggcgg ctttttcttc
     3361 ctcggctcga gtgcccgttg ccctgtcggg ttgcttgccg ctgcgcccgg cctccagccg
     3421 ctgcaatgga tgccaggcga atgtcaccag cgttaatgcc gagagcgcga ggaacccgag
     3481 cagtactgcg ccggcgacac ccacgcggcc ggcgacgact tccgccagca taccgggaac
     3541 caggcccgcg aggcgagccg gcaaggcccg ttgatcgaca tccggaacca gatcgagatg
     3601 ggtcaccttc gcgaccacgc cgacgagata tggcaccagc aggctgaggc cgatcatcag
     3661 cacggccgcc cgcttcatgt cgagtcggcc cagccgctcg aaccccgcca gggcgagccc
     3721 caaaccgagt aatggcagtc ctgtcgcgcc gatgcccagc gcatgccaga gttcgtggcc
     3781 cacccagcgg ccgaccggtc cggtgagctc aaacggaagc agcgtgagcc caacgaagac
     3841 gccgagcacg agggcggtga tggcaccgag gcgatggcga gagtcggtgc tcacgagggc
     3901 tgagcttgcg cggcctgcgg ctgcgattga ggcgcgacgg tcacccggcg ctcgtggaac
     3961 tggggaagaa cgggataggc atcgagcctc ccggcgtcca gcacgtcgtc acggaaaccg
     4021 agtcggatca gttcacgccc ggcccgactg tacgagagtg gacgggtcca gtcgtcgccg
     4081 taccggcgaa ccagatcaag gcttgcgagc gcggcgtcgt tgagtccctt gcgcggcttg
     4141 tgatccccca gggcagcgac ggcgaggcgg ccagcacagt aggcatcgtc caggctgaac
     4201 gcggtttccc gtccggcgca gagtattacc acgtcccgat tctcttccag cgcctcgcga
     4261 gcgcgctcac cggccagcgt caggttcgca gccgaggcga catagacggc cgccgcgccc
     4321 tgggtactca gcaatgcttt ggttccgttc gtcgtcgtca cgacgatagt ctttcccttt
     4381 acggtctgct cggtcatctc gagcgggcta ttgcccagcg cgaaaccggg aatgcgaagg
     4441 caattcttct cgccggccag caggacatcc gcgctgccga tcgtctgggc cagtcggagt
     4501 gcttcttcgg tcgacgcgac cggtatgagc gcccgggcgc cgtgatgaag ggcggcgcac
     4561 atcacggtgg tcgcgcgaag cacgtcgatc acgaagatcg tacggccttg tacgtcggcc
     4621 aggccgagcc cgagcgggga aaaccagaca tgcagcttca tcagtccggc tggagcagat
     4681 gggactcgcg ctcgaggaac cgggtgtcca cttttcccgc cgcgaaatcc gggtgccgaa
     4741 tcacccgggc gagaaacggg atcgtcgtgg tgatgccctc gaggatgaag ctgtcgaggg
     4801 cctggcccat gcgcgacagc gcttcttgtc gagtgttgcc gtgtacgatc accttggcga
     4861 gcagcgagtc gtagtagggc ggaactgtgt agccggcgta cacgtgggtg tccacccgca
     4921 cgccggggcc gcccggcgga tggtacgcgg tgatgagacc gggcgacggc tggaagttcc
     4981 ggtagggatc ttcggcgttg atgcggcact cgatagcatg cccccgcaag cgggtaccgt
     5041 cgccccgata gctaatgggc gcaccggcag cgacctcgat ctgctccttc acgagatcga
     5101 agctcgtcac catctcggtg accgggtgct ccacctgaat gcgggtgttc atctccatga
     5161 aatagaactt accgtcgggg tcgagcagga actcgagcgt tccggcgccc tggtatccga
     5221 tgttcgccgc gagcgccacg gctgactcgc ccatgcggcg ccgaagggcg tgatcgagag
     5281 ccggactcgg actctcctcg atcagcttct ggtggcgccg ctgaaccgag cagtcgcgct
     5341 cgcctaggtg caccaccttc ccatgggaat cgcccagcac ctggatctcg acgtggcgag
     5401 ggcgctcgag gtacttctcc acgtagaccg cgccgtttcc gaaggcggca agcgcctcgt
     5461 tctgcgcgag cccgaacagc tcggagaact gctcgagatc gtgggcgatt cgcatgccct
     5521 ttccgcctcc gcccgcggtt gccttgatga tgacggggaa gccgatctct tgagcaaagc
     5581 gaaccgcttc atcggggtcg tcgattgtcc cgggcgagcc cggcacggtc gggacaccgg
     5641 cttcctgcgc caggcgtcgc gcggtggctt tgtcgcccat cagccggatc tgttcgcccg
     5701 tcgggccaat gaacgtgatg ttcgacgccc gacaggtgtc cgcgaattcc gcgttctccg
     5761 ccagaaatcc gtaccccgga tgaatcgcgt cggcgccggt aatctccgcg gcggcgatga
     5821 ggttcgggat cttgagatac gagagccggc cgggaggcgg accgatgcag acgtcgtcgt
     5881 cggcaaagcg cacgtggagc gactcgcgat cggcctcgct gtagacggcc accgtctgca
     5941 cgccgagctc tcggcaggcg cgaatcaccc ggagcgcaat ttcgccgcgg ttggcgatga
     6001 gaaccttctt gaacatcagg gtatcaagtc ggctgagtca gtgacaagcc tcgagccctc
     6061 catgtgtccg cgggttcgaa agtccggaag gaccggaaag ttagcgcccg ccaccgcctt
     6121 ttggcattcc tcgtacattg atcatcactc ccgagccgca gctggcgcat tccacactag
     6181 gggccacgat cccgccttcc cgttaggcct accggcgcga agccattggc gtgagggacc
     6241 gctcgtcggc ggggcggctt tgccctcgtc ggtggctaca taggcggcgg gatttgcggt
     6301 gcggacgatg gtgcgaatgg cgcggcagcc gatggtgcac cgaggaaata attggcggcg
     6361 gtctggcggg tgcggccgtg gtctccctgg tcggaggact ggcggactcc ctgattcatt
     6421 aaccaatcca gcgcgctcgc ggttgttccc ggaccgagcg ctcagggtgt caggtcgaga
     6481 gacttccgag ccgccgcgtc tccgctcaac tccgagaacg tgcgatccga cgccgaatcc
     6541 acacccgaga cccgaagcgc gaactcgctc gggttcgacg agtagaacag cgcgctttcg
     6601 taggcgacca tcccctcctt gtaccacttc atgagcgact ggtcgaacga ctgcatgccg
     6661 taggacacag tgccctcggc gatcagatcg gggatgttca gcgccttctc gatgtcgcga
     6721 atgttgtcgg ccaccgctgc cgtgttgatg agcacctctg cggcgggaac ccggccgcgt
     6781 ccgtctttgc gaggcaccag tcgcaggcac acgatggcgg cgagggccgt ggagagcaac
     6841 gaccggatct cctggtgctg gtagggtgga tagaacgaga taatccggtt gatggtctgg
     6901 gtcgcgtcgg tagtgtggag ggtggagaag accaggtgac ccgtatccgc ggccttgagc
     6961 gccgtgtcca gggtctccat gtcgcggacc tcgccgatca ggatcacgtc ggggtcctgc
     7021 cgcaggacgt gccgcagcgc ggcgccgaag gacagcgtgt cgctccctac ctcgcgctgt
     7081 gaaatgttcg actgcacgtc gcgatggagg aactcgattg gatcctcgat cgtgatgacg
     7141 ttgcagcggc ggtgctggtt cacgtggtga atcatcgagg ctagcgccgt cgacttcccc
     7201 gatcccgtga tacccgtgac cagaacgatg ccgcggggct tgagcgagat ctcctccagg
     7261 atcgccggga gattcagctc ccggatggtc ttcacttcgt agggaatcgc gcggaatgcg
     7321 aacgccagag taccacgctg ctgatagatg ttggtccgga accggcccac gcccggcaca
     7381 ccgatcgcga agtctgcttc cttcttctcg gcaaactcct tcacctgacg cggcgtcatc
     7441 acctgctcag cgagcagctt caggtcttcc ggtttgagcg ccgacatctc gagcgtagtc
     7501 aactccccgt tgactcgcac ggtgggcgct cgccccacct tgaggagaag gtcggagcca
     7561 ttgcgctgga tcatctgcgc aatcgcttgc ttgaaattga acgccgcgac ggcgcccggt
     7621 gccgttgcct cagccattcg ggtccacccg gaaaaggacc tggccaaact ccaccggctg
     7681 cgcgtcctcg acagatacct cacggaccac gccggcgatc tccgcctcga tctcgttcat
     7741 gatcttcat
//
LOCUS       DASRJQ010000249         5076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_52674, whole genome shotgun sequence.
ACCESSION   DASRJQ010000249 DASRJQ010000000
VERSION     DASRJQ010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5076
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52674"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VFB89_05645"
     CDS             <1..401
                     /locus_tag="VFB89_05645"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HZM26817.1"
                     /translation="TASADTSGKAVATIPPDSGSVASALPGAADTTPLPAADTTRRPA
                     ADTAGKARTDSASTKASYRIQVAAVNTRAAADSIARRTKAAGFESVVASEKGLFKVRV
                     GRYATRAEAQAALPGVRSKFGGQPFIVAAS"
     gene            398..3001
                     /gene="mutS"
                     /locus_tag="VFB89_05650"
     CDS             398..3001
                     /gene="mutS"
                     /locus_tag="VFB89_05650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HZM26818.1"
                     /translation="MTGRVAEGTTPLLQQYREIKARHPGAILFFRMGDFYEMFFDDAQ
                     LGARLLNITLTSRGDGVPLAGVPVKAAADYLKQLVAAGQRVAICEQVEDPRLAKGLVR
                     REVVETVTPGALLVEGWLPGSRNNWIVAVGVKNVTESAGLAAIDLSTGEFVLETVSTG
                     GLEEGLSRFNPAELVVPAGAAELRAGSTSEQCLVTQREAWEFDPDLARAELTRRLGVT
                     SLDGFGVEVGDARAVGAAGALLRYLGELQPGGLPHLARPTVRRSQAHLWIDEMTRRNL
                     ELVEPLRPGMRGATLLETVDLTLTPMGARLLRGWLLSPLSDPKAIAARLDAVETMVQD
                     NRGRGRLREALEGVRDLERLAGRAAAARATPRELAALRDSFRRLPDVLESLVTLSGRD
                     RSAALGEVESRFDLLADLAQVLGDALADRPPALLADGGVIRPGHDAELDELRDLRDGG
                     KQYIAMLQQRERDRTGIPSLKVGFNKVFGYYLEITNAHATKVPPDYERRQTLASAERY
                     VTPELKTYEAKVLGAEERMGIREAELFAALRNRVGGAIDRIQRTARALARLDVWSALG
                     EVAVQYRYVRPVVDDSFGLMLRQSRHPVIERLIPREQFIPNDVRFAESERVLLVTGPN
                     MAGKSTILRQIGLCVVLAQMGGFVPAEAAHIGAVDRLFTRVGASDNLARGQSTFMVEM
                     SETSAILHNATARSLVLLDEIGRGTSTYDGVAIAWAVTEHLHDRIGCKTMFATHYHEL
                     MQLPEQLEHARNLNVAVRETGETVVFLHRLEPGGTDRSYGIHVAGLAGLPSQVVSRAK
                     EVLATLEGEHRVVAGAPAPPADPGQLTLFAADPGSDPLMQEIRGLDLDAMTPLEALNR
                     LADLQRRAEGR"
     gene            2998..3411
                     /locus_tag="VFB89_05655"
     CDS             2998..3411
                     /locus_tag="VFB89_05655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HZM26819.1"
                     /translation="MIGRGSTALLALAMVLPGRCARSDDATVRLPTERPGSAPPPESP
                     PVALNAESPVGFPPALLTQGVEGIVVLHLFVDANGMAAAESTRVAESSGYPALDSAAL
                     AAVPQLHYAPAMHAGTPVATAFLQPIQFRNPQGRK"
     gene            3416..4582
                     /locus_tag="VFB89_05660"
     CDS             3416..4582
                     /locus_tag="VFB89_05660"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZM26820.1"
                     /translation="MSEPQHLNPYQSVLDTIGWTPLIRLARIGAGVRTPIYGKAEHKN
                     PGGSVKDRIGIAIIEAAEANGRLKPGGTIVEATSGNTGIGLAMAAAIKGYRCVFTMPD
                     KMSQEKVRMLKAYGAEVVVTPTAVPPEHPDHYRAKAQQIVRETPGAILADQYFNPVNP
                     EVHYRTTGPEIWAQTGGKITHFVVAAGTGGTVSGAAKFLKENNPKITVISGDPEGSVY
                     AGFARTGETCGGAPYKVEGIGGDAIPANIWFDYIDEFRTVSDRTAMLMTRRLAREEGI
                     LVGSSSGVNVALALDVAREVDDQAAVVVTVLCDTGERYLSKVFNDEWMRENQLLDSPR
                     VTVEQLLSRRPSGAPPLVSVAPAAAVRQALGLMNTWDVSQIPVVDGDEAIGTLL"
     gene            4592..4807
                     /locus_tag="VFB89_05665"
     CDS             4592..4807
                     /locus_tag="VFB89_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZM26821.1"
                     /translation="MTRALSQPALLDRPVREVMDPPLPIVDAHFPTDRLGAMLTRESP
                     AALVRKGGKLVGIVSRYDVLQQLIGAR"
     gene            4814..>5076
                     /locus_tag="VFB89_05670"
     CDS             4814..>5076
                     /locus_tag="VFB89_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HZM26822.1"
                     /translation="MSRITVLTGGTSTERDVALASAAQVIAALRACGHEVSVVDTARG
                     YIPQGEEATLVASSVGREPPKLADLKALERGVLLSGLGNIPVVR"
BASE COUNT          891 a         1612 c         1693 g          880 t
ORIGIN      
        1 acaccgcatc ggcggatacg agcggcaagg cggttgctac gattcctccc gactctggga
       61 gcgttgcgag tgctctgccc ggagcagccg acaccacacc gcttcctgcc gccgacacca
      121 cacggcgccc tgccgccgac actgccggca aggcgcggac cgactcggct tccaccaagg
      181 cgagctaccg catccaggtt gccgcggtca atacccgcgc cgcggccgac agcatagccc
      241 gcaggaccaa ggccgccggg ttcgagtctg ttgttgcgtc ggaaaaaggg ctgttcaaag
      301 tccgggtggg ccgatacgcg actcgcgcgg aggcccaggc tgcgctgccc ggcgttcgct
      361 ccaagtttgg tggccagccg ttcatcgtgg cggcgtcgtg actggtcgcg tagcggaagg
      421 aacgactcct ctgctccagc aataccggga gatcaaggcg cgtcatccgg gcgccatcct
      481 gttcttccgg atgggcgact tctacgaaat gttcttcgac gacgcgcagc tcggtgcccg
      541 cttgctcaac atcacgctca catcccgggg tgacggcgtc cctctcgccg gcgtgccggt
      601 caaagcggcg gcggactact tgaagcaact ggtcgccgcg gggcagcgtg tcgcgatctg
      661 cgagcaggtg gaggatccca ggctcgcgaa gggactcgtt cgacgtgagg tagtcgaaac
      721 tgtcacgcct ggcgccctgc tggtcgaagg ttggcttcct ggaagccgca ataactggat
      781 cgtcgcagtc ggagtgaaga acgtcaccga gtcggccggt ctggccgcga tcgatctcag
      841 caccggcgag ttcgtcctcg agacggtaag caccggcggt ctggaagagg ggttgtcccg
      901 tttcaatccc gccgagctgg tcgtgccggc tggagccgcc gaattacgag caggctcgac
      961 cagtgagcaa tgcctggtca ctcaacggga ggcctgggag ttcgaccccg atctcgcccg
     1021 ggcagagctg acccggcggc tcggtgtcac ctctttggac ggatttggag tcgaggtggg
     1081 agatgcgcgc gcggtgggag ccgccggggc gctgctgcgc tatctcgggg agcttcagcc
     1141 cggcggcttg cctcacctgg ctcgtccgac agtgcgccga agccaggccc atctctggat
     1201 cgacgagatg acccgccgca atctggagct ggtcgagccg ttgagacccg gcatgcgagg
     1261 cgcaacgctg ctcgaaaccg ttgacctcac ccttaccccg atgggggcgc gcctcctgcg
     1321 cggctggttg ctctctcctc tgtccgatcc gaaggcaatc gctgcccggc tcgatgcggt
     1381 cgagaccatg gtgcaggaca accggggacg tggtcgcctc cgcgaagcac tggaaggcgt
     1441 ccgcgatctc gagcggctcg cggggcgagc cgccgcggcc cgggccactc ccagggagct
     1501 cgctgcgctg cgcgactcgt tccggcggct gcctgatgta ctcgagtcgc tggtgacgtt
     1561 gtctggtcgc gatcggagcg ccgcgttggg agaggtcgag agtcggttcg acctgctcgc
     1621 cgacctcgcg caagtactcg gcgatgcctt ggccgaccgg ccacccgcgc tcctggcgga
     1681 cggcggcgtt attcgccccg gccatgacgc cgagctggac gagttgcgcg acctgcgcga
     1741 cgggggaaag cagtacatcg ccatgttgca gcagcgggag cgcgatcgca ccggcattcc
     1801 gtcgctcaag gtgggcttca acaaggtctt cggttactac ctcgagatca ccaacgccca
     1861 cgcgaccaag gttcctccgg attacgaacg gcggcagacg ctcgcgagcg ccgagcgcta
     1921 cgtgacaccg gagctcaaga cctacgaagc gaaggtgctg ggtgccgaag agcggatggg
     1981 gattcgagag gcggagctgt tcgcggcgct cagaaatcga gtcggtgggg caatcgatcg
     2041 aattcagcgc accgcgcggg ctctcgcccg gctcgacgtg tggagcgcat taggcgaagt
     2101 ggcggtgcag taccgctacg tccgccccgt ggtggacgac agttttggcc ttatgctccg
     2161 ccaaagccgg cacccggtga ttgagcggct cattccgcgc gagcagttca tcccgaacga
     2221 tgttcgattc gccgaatcgg agagggttct gctggtcacc ggcccaaaca tggcaggtaa
     2281 gtcgaccatt ctacgccaga tcgggctctg cgtcgtgctc gcccagatgg gcggctttgt
     2341 ccccgcggaa gccgcccata tcggcgccgt ggatcggctg ttcacccggg tgggcgcgag
     2401 cgacaacctg gcgcgtggcc agtcgacctt catggtcgag atgagtgaga ccagtgcaat
     2461 cctgcacaat gccactgcac gtagcctggt cctgctcgac gaaattggac gaggaacgtc
     2521 tacttatgac ggtgtcgcaa tcgcctgggc cgtaaccgag cacctgcacg atcggatcgg
     2581 ctgcaagacc atgtttgcga ctcactatca tgagctgatg cagctgcctg aacagttgga
     2641 gcacgcccgc aatctgaatg tcgcggtgcg ggagaccgga gagaccgtgg tgttcctcca
     2701 ccggctcgag ccgggcggaa ccgatcggtc ttacggcatt catgtggccg ggcttgccgg
     2761 gttgccctcc caggttgtct cccgagcgaa agaagtgctc gccaccttgg aaggggagca
     2821 ccgggtggtg gcgggtgcac ctgcgccacc cgctgatccc ggacaactca ccctcttcgc
     2881 ggcggacccc ggctcggatc cgttgatgca ggagatccgg ggactcgatc tcgatgcgat
     2941 gaccccgctc gaagccttga atcggttggc ggatctgcag cggcgggctg agggccgatg
     3001 ataggacgcg gctccactgc gctgcttgcg ttggccatgg tgctgccagg ccgatgtgcc
     3061 cgctcggacg acgctacggt tcggctgccg acggaacgcc cggggtcggc tccgcctccc
     3121 gaaagccctc cagtggcgct caacgcggag tcaccggtcg gctttccccc cgccttgctc
     3181 acgcagggcg tcgagggaat cgtggtgctg cacctctttg tcgatgcgaa cggtatggca
     3241 gcggcagaat cgactcgagt agccgaatcc agtgggtatc cggcattgga ctcagctgcg
     3301 ctggcggcgg tgccacagct gcactacgcg cccgcgatgc acgcgggtac gccggtcgcc
     3361 acagcgttcc tgcagccgat tcaattccgg aatccgcagg gaagaaagta ggagtatgtc
     3421 cgagccccag catctgaacc cgtaccaatc ggtgctcgac accatcgggt ggacgccgct
     3481 gatccggctt gcccgaatcg gcgccggcgt ccgcacgccg atatatggca aagcggagca
     3541 caagaatccg ggcggctcgg tgaaggatcg cattggaatc gcgatcatcg aggccgcgga
     3601 ggcaaacgga cggctcaagc caggtgggac catcgtcgag gcaaccagcg ggaacaccgg
     3661 gatcggactc gccatggcgg ccgcgatcaa gggctatcgg tgtgtcttca ccatgccgga
     3721 caagatgtcg caggagaagg tccggatgct caaggcatac ggtgcggagg tggtggtgac
     3781 acccaccgcc gttccacccg agcacccgga tcactaccga gccaaggctc agcagattgt
     3841 gcgggaaacc cccggtgcca ttctcgccga ccagtacttc aatccggtca atcccgaggt
     3901 ccactaccgc accaccggcc cggagatttg ggctcagacc ggaggaaaga tcactcactt
     3961 tgtggtagcc gcaggcaccg gtggtaccgt gagcggcgct gcgaagttcc tgaaggagaa
     4021 taaccccaag atcacggtta tctccggaga ccctgaaggg tccgtctacg cggggtttgc
     4081 ccggaccgga gagacctgcg gcggcgcccc ctacaaggtg gaaggcatcg gcggcgacgc
     4141 catccccgca aacatctggt tcgactacat cgacgagttc cggaccgtca gcgatcgaac
     4201 ggcgatgctg atgacccgcc gtctcgcgcg ggaagaggga attctggtgg ggagttcctc
     4261 gggcgttaac gtggcgttgg cgctggacgt ggcccgcgag gtcgacgatc aagcggctgt
     4321 cgtggtcacg gttctttgcg ataccggcga gcgctaccta tcgaaggtat tcaacgacga
     4381 atggatgcgg gaaaatcagt tgttggattc acctcgcgtt accgtggagc agctgctcag
     4441 ccggcgtcca tcgggcgccc cgccgttggt aagcgtcgct cctgccgcgg ccgtacggca
     4501 ggccttgggc ctcatgaata cctgggacgt gagccagatt cccgttgtcg atggcgatga
     4561 ggccatcggc actctgctct agaacacctt gatgactcga gcgctttccc agcccgccct
     4621 actggatcgg cccgtccggg aggtgatgga tccgccgctt cccatcgtcg atgcccattt
     4681 cccaactgat cgattgggag caatgctgac ccgggagagt cccgccgcgc tcgtccgcaa
     4741 gggcggaaag ctggtgggca tcgtcagccg ttacgacgtt ttgcagcagc tcatcggggc
     4801 ccggtaaccc gacgtgagcc gcatcacagt cctcaccggc ggcacctcga ccgaacggga
     4861 tgtcgcgctg gcgtccgcgg cacaggtcat cgcggcgctc cgcgcctgcg gccacgaggt
     4921 ctcagtggtt gataccgcgc gagggtacat cccccagggc gaagaagcca ccctggttgc
     4981 gagcagcgtg ggacgagaac cccccaaact ggccgactta aaggcgctgg aacggggtgt
     5041 gttactgtcg ggtctgggca acatcccggt ggtgcg
//
LOCUS       DASRJQ010000250         2367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_901857, whole genome shotgun sequence.
ACCESSION   DASRJQ010000250 DASRJQ010000000
VERSION     DASRJQ010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901857"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..811
                     /locus_tag="VFB89_05675"
     CDS             110..811
                     /locus_tag="VFB89_05675"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HZM26823.1"
                     /translation="MEQMRNEGSVSRRAVLGSSVAAALGATLGISSWTGRFPSVAAQT
                     NISPADALKRLMDGNARYAIGKLISFQQDLQILKQKTTEKQEPFAAVLSCADSRVPVE
                     IVFDQTIGHVFVTRVAGNVVTPEIIGSLEYGVAVLGTAVLVVLGHGSCGAVSAAIAAK
                     DVPGQISALYRHIRPAVDQAGSDLDAAIRANARIHARLLQEASPVIASAVKQKRLKVV
                     AAYYDLATGVVTLLG"
     gene            complement(1113..>2367)
                     /locus_tag="VFB89_05680"
     CDS             complement(1113..>2367)
                     /locus_tag="VFB89_05680"
                     /inference="COORDINATES: protein motif:HMM:NF025433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HZM26824.1"
                     /translation="AVPVYSGRMLAPLIVALALAARSPDSLIVPAGTPVPVRFLSPIK
                     SGSTRRGWTVEVQTMADVVAGRCVAIPAYTQILGIVDESLAGWFFRHRGRLHLNFQIV
                     QTGPADWALLTAVVDDIEWLALNLVDSSGLIRPAQHSTGHRLLGGSSRFAVEERILTP
                     LTGAVAAPVAVAGTGAGFVARGPRVEIREGDEATLRLTSPFRLAADGPCRTVLPLSDS
                     VTTPGLDLSSLPPRTTGGNGAPADVINLLILGRRTDLERAFVEAAWLEASAGGLRSRV
                     GAAGAVVLGKSNKRGPVSPQRLFGRTEDLAFERQGLNARQRHHVRIWAVDSNETIWVG
                     AANEDVGVYVTVAKPRITHRIDSRIDGERDLLSRELEASGCAVREGFVRIPGCPLSGQ
                     NSSGEGFVTDTRVAVVRLRACRSPP"
BASE COUNT          445 a          780 c          760 g          382 t
ORIGIN      
        1 aaggcggcgc cggagaacag tgaacgcgct gcctcctcgc tgcgcttccg aactcaggag
       61 ttatcggcat tcacttaaac attcgcatcc gaggccaacg gaggacgaca tggagcagat
      121 gcgtaacgaa gggagtgtct cgcgtcgcgc agttctcgga agctcggtgg cagccgctct
      181 cggcgccaca ctcggtatct cgtcgtggac ggggcggttc ccgagcgtgg ccgcgcagac
      241 caacatcagt cccgccgatg ccctgaagcg gctgatggac ggcaacgccc gctatgcgat
      301 cgggaagctg atttcgtttc agcaggacct tcagatcctc aagcagaaga cgaccgagaa
      361 gcaggagccg ttcgcggcgg tgctctcgtg cgcggactcc cgggtgccgg tcgagatcgt
      421 ctttgatcag acgattggcc acgttttcgt cacgcgggtg gccggcaacg ttgtcacgcc
      481 cgagatcatc gggagcctcg agtacggcgt ggccgtgctt gggaccgccg ttctcgtggt
      541 gctcggccac gggtcctgcg gggcggtgag cgccgcgatt gcggccaagg acgtgcccgg
      601 gcagatcagc gcgctgtacc gccacattcg gccggcagtg gaccaggcag gctcggatct
      661 cgacgccgcc atccgggcca acgcccgcat ccacgcccgc cttctgcagg aggcctctcc
      721 agtgatcgcc agcgccgtca agcaaaaacg cctgaaggtg gtcgcggcct actacgacct
      781 cgccaccgga gtggtcacgc tccttggcta ggttgatccg gaccgggcta ccaccagcgc
      841 accccaccgc tgacgtgagc acgacaggtg ctaccagtgt cagggatgac aagacaatgc
      901 tttaccgcat tggcgtaatg cggctgaggc cgggttcgag gccgtgcagg gctgatgtgg
      961 aggggatcgt agccaaacgg gattactcgt agtgttctgc cggcgccccg ctggccaatg
     1021 gggaacgcat gagcactacc aggcggctcg tcagggtcag gcgtcaccag agccaccggt
     1081 gagtacactc tcgcaaatat cagggaacgg ccctagggcg gtgaccggca ggcccgcagc
     1141 cgcacgaccg ctacccgcgt gtccgtcacg aatccctctc cgctcgagtt ctggcccgac
     1201 aacgggcatc caggaattcg gacgaagccc tcccgcacgg cgcaccccga tgcttcgagc
     1261 tcgcgtgaga gtagatcacg ctcgccgtca atccgggaat caatccgatg ggtaatcctg
     1321 ggcttggcga cggtgacata gacaccgacg tcttcgttgg ccgcccccac ccaaatcgtc
     1381 tcgttggaat ccacggccca aatccgaacg tgatggcgct gccgtgcgtt cagtccctgg
     1441 cgctcgaatg cgagatcttc ggtccgacca aacaagcgtt gggggctcac tgggccccgc
     1501 ttattcgatt tgcccaacac gaccgcacca gcggctccaa cccggcttcg aagtcctccc
     1561 gcagacgcct cgagccatgc cgcctcgacg aacgcgcgct cgaggtcggt tcgcctcccc
     1621 aggatgagaa ggtttatcac atctgcgggg gcgccattcc ccccggtagt gcggggtggc
     1681 aaactgctca ggtcgagccc cggcgttgtt acggaatcgc tgagtgggag aacggtccgg
     1741 cagggcccat ccgccgctag tcggaacggc gacgtgagac gcagcgtcgc ctcgtcgccc
     1801 tcacgaattt cgacccgggg ccctcgggcc acaaagcccg ccccggtgcc tgcgaccgcc
     1861 acaggcgccg ccacggctcc tgtgagcgga gtcaggatcc tctcctccac cgcgaagcgg
     1921 ctgctcccgc cgagtaaccg gtgaccagtg gagtgctggg cgggtcggat gagcccacta
     1981 gagtccacca aattgagggc cagccactca atgtcatcca ccaccgcagt cagcagcgcc
     2041 cagtctgcag ggccggtttg cacgatctga aagttcaggt gcagccggcc ccgatggcgg
     2101 aaaaaccagc cggccaacga ttcgtccacg atcccgagga tctgggtata agcagggatg
     2161 gctacacagc gaccggcgac gacatcggcc atggtctgaa cctcgacggt ccagccgcgt
     2221 cgagtggacc cgctcttgat cggcgagagg aagcgcaccg gcacaggagt tcctgccggc
     2281 actatcagcg aatcggggga ccgtgccgcg agcgcgaggg cgacgatgag cggcgcgagc
     2341 atacgaccgg aatatacggg aacggcg
//
LOCUS       DASRJQ010000251         3211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_223811, whole genome shotgun sequence.
ACCESSION   DASRJQ010000251 DASRJQ010000000
VERSION     DASRJQ010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223811"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..258)
                     /gene="rpsT"
                     /locus_tag="VFB89_05685"
     CDS             complement(4..258)
                     /gene="rpsT"
                     /locus_tag="VFB89_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HZM26825.1"
                     /translation="MPRIRSSKKRMRQSRARTLQNRTKRSQLWTAIKKVRAASSAADA
                     KQAYSDAVKLLDRAGRKNLIHPNAAARHKSRLAKLAGGKK"
     gene            359..1108
                     /locus_tag="VFB89_05690"
     CDS             359..1108
                     /locus_tag="VFB89_05690"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZM26826.1"
                     /translation="MTSTCPPYNLLAAFSGTPPLLHDIILALPVLVFSLVAHEYAHGA
                     AALRQGDDTAYMLGRLTLNPVPHIDPIMTVIMPALLLLVSGGSFAFGGAKPIPVNPRK
                     YRHFKRGDIIVSAAGVVTNLAIALACALLFVALGFLTELVPGIAFVADTAQRMMMWGV
                     WLNLILCFFNLIPIPPLDGSHLFYYLLPPNLGAQYRSIQRFGILPLLALMLLAPGLFH
                     WLLGPAYYGMSLLQHLIVPFAVGDGWNIFSA"
     gene            1105..1857
                     /locus_tag="VFB89_05695"
     CDS             1105..1857
                     /locus_tag="VFB89_05695"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HZM26827.1"
                     /translation="MTAPNAVPPTIALGAEPGFVVQLEAFSGPLDLLLHLLREEKIEI
                     ADIPIARICDQFLKAIHELGLNQAADYLEMASRLVRLKVQLLLPRNLDDDEWEDPRAE
                     LVRRLLEYQQIREIAQWMGSAVERRTERLARGYLPPPPDLPPLPLTLDLLGLLQAVDR
                     VIAAIPIPVLHRVVARPLDVEGATRRIEALLEAHGEIGWLDALGPEPTIADLLSVLLA
                     LLELARRGVLQLRQPAPFAPMVIARESAHPAV"
     gene            1832..2416
                     /gene="scpB"
                     /locus_tag="VFB89_05700"
     CDS             1832..2416
                     /gene="scpB"
                     /locus_tag="VFB89_05700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HZM26828.1"
                     /translation="MNPLTQLCEAALFSAARPLTVEELATLDPEATLADVRTALDELR
                     EHYDFNQHGVELVELAGGYQILTRPVHAAAIERAQFSARAPRLTAAALETLAVIAYRQ
                     PVGRAEIEEIRGVSAGGVLRTLQERGLLEVVGRSEALGRPLLYGTTPLFLELLGLRDL
                     TDLPKAEELTIALHPHRPEAQGPDDAEAYVPAES"
     gene            2422..3144
                     /locus_tag="VFB89_05705"
     CDS             2422..3144
                     /locus_tag="VFB89_05705"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HZM26829.1"
                     /translation="MRLQRALAQAGVASRRAAERLIEEGRVRVDGQVATLGSKVDPER
                     QAITVNGRRIRALLRRWLAFHKPLGVVTTASDEAGRRTVFDFIRDPAGLTYVGRLDVN
                     TTGLLLLTNDGEAVHRLTHPRFRVPRHYTALVHGRPLEEIREAVKRRVLIEGRPVVTS
                     DVRVRPGREGRSIVDVTLGEGRHRIVRRWCEALGLRVDRLARTSYGPVRLGDLAPGEW
                     RVLSEREVAGLYRAVGLGTGAD"
BASE COUNT          505 a         1045 c         1008 g          653 t
ORIGIN      
        1 gtcctacttc ttccctccag ccagcttcgc taaccggctc ttgtgccgcg cagctgcgtt
       61 ggggtgaatc aggtttttgc gaccggcccg gtccagcagc ttgaccgcgt cactataggc
      121 ctgcttcgcg tccgcagccg agctcgccgc acgaaccttc tttatcgcgg tccacagctg
      181 gcttctcttg gtgcggttct ggagcgtgcg ggcccggctc tgccgcatgc gcttcttgga
      241 agacctgatt cttggcacga cgacctcagt tcgagcgacg tttctggctt cttaagccac
      301 ggggaaagcg ggccgaagaa taggcctgag acccgccaag gtcaagcgga gtcgcgtttt
      361 gacttcgact tgcccgccgt ataaccttct cgcggccttc tccgggaccc cgccgctgct
      421 tcacgacatc atcctagcgc tgccggtgct ggttttctcc ctcgtagcgc atgagtatgc
      481 ccacggcgct gcggcgctcc ggcagggcga cgatactgcc tacatgctgg ggcggctcac
      541 cctgaatccg gttcctcata tcgatcccat catgacggtg atcatgccgg cgctgctgct
      601 cctcgtatcc ggcggatcat tcgcgtttgg gggtgccaag ccgattccgg tgaatccgcg
      661 gaaataccgg catttcaagc gcggtgacat catcgtgtcg gcagccggtg tcgtcaccaa
      721 cctggccatc gcgcttgcgt gcgccttgct gttcgtcgca ctcgggttcc tcaccgagct
      781 ggtccctggc attgcgttcg tcgctgacac ggctcagcgc atgatgatgt ggggcgtctg
      841 gctcaatctc attctctgtt tcttcaacct gattccgatc ccgccgctcg acggctcgca
      901 ccttttctac tatctcctgc cgccgaatct cggcgcgcag tatcgatcga ttcagcgatt
      961 cgggattctg ccgctgctcg ccctgatgct gctggcgcct gggctattcc attggctgct
     1021 gggtccggcg tactacggaa tgagtctcct ccagcacctc attgtgcctt tcgccgtagg
     1081 cgacggctgg aacatcttca gcgcatgacc gcgcccaatg cggttccccc gacgattgcc
     1141 ctcggcgccg aaccggggtt cgtagtgcag ctggaggcgt tctccggtcc cctcgatctg
     1201 ctgcttcatt tgctgcgcga ggaaaaaatc gagattgccg acattccgat cgcccgcatc
     1261 tgcgatcagt ttctcaaggc aatccacgag ctcggtctca atcaggcggc cgactatctc
     1321 gaaatggcga gccggctggt gcgtctcaag gtccagctcc ttctgccaag gaatctcgat
     1381 gacgatgaat gggaggatcc tcgggcggag ctggtccgaa ggttgctcga gtatcagcag
     1441 atcagggaga tcgcgcagtg gatgggatcc gctgtagaac gccggaccga gcgactcgct
     1501 cgaggctatc tgccgccccc tcctgatctg cctccgctgc cgctgacgct cgatcttctg
     1561 ggtctgctgc aggccgtcga tcgggtaatt gccgccatcc caattccggt tctgcatcgg
     1621 gtggtcgctc gcccgctcga tgtcgaaggc gccacacggc ggatcgaagc tctgctcgag
     1681 gctcacggcg agatcggttg gctagacgca ctgggcccgg agccgacgat cgccgacttg
     1741 ctgtctgtcc tccttgccct gctcgagctg gctcggcggg gcgtgcttca actccggcaa
     1801 ccggcgccct ttgcgccgat ggtgatcgcc cgtgaatccg ctcacccagc tgtgtgaggc
     1861 ggcgctcttc tccgccgcgc gaccattgac ggtggaagag ctcgcgaccc tcgatccaga
     1921 agcaaccctg gccgatgtgc gaaccgcgct tgacgagctg cgcgagcact acgatttcaa
     1981 ccagcacggt gtggagttgg tagagctggc cgggggttac cagatcctga cccggccggt
     2041 acatgccgcg gcgatcgagc gggcccagtt ctcggcccgt gctccgcgtc tcaccgctgc
     2101 agccctcgaa acgctggccg tcatcgccta ccgacaaccg gtcggccgag ccgagatcga
     2161 agagatcaga ggcgtgtcgg ccggcggcgt attgcgtacg cttcaggagc ggggactgct
     2221 cgaagtcgtc ggccggagcg aagctctcgg gcgaccactg ctgtacggga ctacccctct
     2281 gtttctcgag ctgctcggcc tccgcgattt gactgatctg ccgaaggccg aagagctcac
     2341 catcgcgctc caccctcatc gcccggaagc ccagggaccg gacgacgccg aagcctacgt
     2401 gcccgctgaa tcgtgagcga aatgcggctc cagcgcgcct tggcgcaggc cggggtggcg
     2461 tcgcgccggg ccgcggagcg gctgatcgaa gaaggacgag tgcgagttga cggccaagtc
     2521 gcgacgctgg gatcgaaggt cgatcctgag cgacaggcca tcactgtcaa cggccgcaga
     2581 atccgggcac tgctccgacg ctggctggca tttcacaaac ctctcggggt ggtgacgacc
     2641 gcctcggacg aggccggacg gcgaactgta ttcgatttca ttcgcgatcc ggccggacta
     2701 acctacgtcg gccgtttgga cgtgaacacc acgggcctcc tgttactgac caatgatggc
     2761 gaagcggtcc accggctgac ccacccgcgg ttccgtgtcc cgcggcacta cacggccctg
     2821 gttcacgggc gccccctgga ggagatccgc gaggccgtga aacgacgcgt gctgatcgaa
     2881 ggtcgtccgg tcgtcacctc cgacgtgcga gtccggccgg gccgggaggg gcgcagcatc
     2941 gtcgatgtga cgctggggga aggcaggcac cgcatcgtgc ggcgttggtg cgaagccctg
     3001 gggctgcggg tagaccggct cgcgcgtact tcatacgggc cggtccggct tggcgatctc
     3061 gcacccgggg aatggcgcgt cctgagtgag cgagaggttg ccggattgta ccgggcggtc
     3121 ggccttggta ccggagctga ttagtcgcca cctctgatag ctgctagctg ctagctgcta
     3181 gctgctagct gctagccgat agctgatagc t
//
LOCUS       DASRJQ010000252         5059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_4002685, whole genome shotgun sequence.
ACCESSION   DASRJQ010000252 DASRJQ010000000
VERSION     DASRJQ010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4002685"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            322..1245
                     /locus_tag="VFB89_05710"
     CDS             322..1245
                     /locus_tag="VFB89_05710"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZM26830.1"
                     /translation="MPSLPVLLAILLLATSVRAQSRPAESRPPNPLGRVPILEYHLIA
                     EQDSRWGRSRDGFRRDLELLYQRGYRPVTVSELMDRQVDLPAGTSPVVVTFDDASPGQ
                     FRYLERDGRLDIDPESAVGIWLDFTARHSDWRPRATFCLLPAASAGHAFFGEKNIEGQ
                     KTAWRFPKLRFLQEKGFELCAHTLWHANLSTLSDAGVQEQIARSLIAIDSAVPGLRVR
                     TFALPLGIWPKNKALAHEGTWRDPKTGRVVNYRFDAILEVSGGAARSPAAPEFDPLRL
                     PRIQVIGDNLARELDRLEKDRYVAAGSGSTH"
     gene            1208..2362
                     /locus_tag="VFB89_05715"
     CDS             1208..2362
                     /locus_tag="VFB89_05715"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HZM26831.1"
                     /translation="MVTSPPVADPPTSGRLVSLDVFRGLTVAAMLLVNDPGSWGHIYP
                     PLRHAAWHGWTPTDLIFPFFLFIVGVTTHLSLGARRARGADENQLAAQVLRRGAIIIV
                     LGLLLSAFPFFPLERIAEIRIPGVLQRIGLVYLATGLLALRTTVRSEIAIAVIILVGY
                     NLLLVVVPVPGVGHPALAPPDSTLAAWVDRRVLDGHLWAVTRTWDPEGLLPTIPAIAT
                     CILGLLAGRWITRSGPREGRIGGLLAAGGAGITAGWVWNAALPINKNLWTSSYVVFTA
                     GLACVCLALCMWLVDGRGPRWWTRPLVVFGINPIVAFVGAEALARLLYSVIRVSYDGE
                     PVPLQVAIYRSLFASWPSPEAGSLGFALLIVLFWYGVLEVLYRRGLVIRI"
     gene            complement(2352..2831)
                     /locus_tag="VFB89_05720"
     CDS             complement(2352..2831)
                     /locus_tag="VFB89_05720"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZM26832.1"
                     /translation="MTDGAEFVEQVGVFFEAEGFSRTAGRIFGRLLLSEKSLSLDQLA
                     ADLGVSKASISTETRMLERRGALERTGQAGDRRVYYRVAPGLPVPTLELRLERMRRFS
                     RLIGEARQGLRRGNPVIRQRLELMAEAHRYVVSTLGAALERWHASNRTGRRRIALKS"
     gene            2969..4447
                     /locus_tag="VFB89_05725"
     CDS             2969..4447
                     /locus_tag="VFB89_05725"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZM26833.1"
                     /translation="MPRLIGCHVASAYAVVLLLASCAPPLPGPGGGAPAVAPAPHIKW
                     TPPREAPAEQAQPAPAIPPDIAERIQRLTMGDVVDLALRGNPATAQAWANARAAAAAY
                     GASRSAYYPTLDADYSVTRIKTAATSGRVAVSQTVYGPSVTLSWLLWDFGGRSGSIES
                     ARQGLLAADWTHNATIADVVLQAQVAFFNYIATGAFLTAQRASVKDAEANLEAADARR
                     QVGAATIADVLQARTALSQARLALDTLEGSRLTARGALAVSLGLSANVPYDVDSVRAL
                     RPIAGVSDSVEAIINRAVRDRPDLAAARAEAEAARARIREIRGSRLPSITATGTAAHT
                     DVAGRGSAGNSYNIGVGLAVPLFNGFGREYSQKQAEYLADAAAAQQRSLEQQVTYQVF
                     SAYYALQTATRRVQTSNDLVASASQSTEVALARYKAGVGTVLDLLAAQGALAAARSQV
                     VQGRLAWYISLAQLAHDAGALDPRGGTDLRMSPDTVESIPRQ"
     gene            4444..>5059
                     /locus_tag="VFB89_05730"
     CDS             4444..>5059
                     /locus_tag="VFB89_05730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZM26834.1"
                     /translation="MKRTFLLFLTAFAALSSACNRTPRRELPPVPVVAATAERRSVPF
                     TLEANGTVEPLETVAVESQVTGYLLRVGFKEGDEVRPGQVLFEIDPAPYRAALDQARA
                     VLARDQAQLTAAKQDAQRYEQLAAKEYVTTQQAEQARATAGAQAASVKADEAAVESAR
                     INRDNATIRSPIGGKAGGLLVKPGNLVRGGAGVPLVTINRIHPIL"
BASE COUNT          811 a         1685 c         1660 g          903 t
ORIGIN      
        1 tcgagtcacc ccgacagaga gcgatgagcg ctgggcgaga agttgggtca aactcccgtg
       61 ggcgaggtcc actgcccccg tcaccatggg atggcctggc ggataggcag cactcttgtg
      121 aagtgcggtc gagagctgta cgagaaacgc cgagaggtcg cgatccacgc ggattcaacc
      181 ccgggcttca ggcatcgttg tcgcaacggg atcgagtgta tcctgtcggg acgccctagt
      241 gcgggtcaag cgccatcgtg tcaattgacg aaccgacgac cctggcccgg atccggccgc
      301 ctcgcctaat ttcctcgctg gatgcccagc ctgccggtat tactggccat acttctgctt
      361 gccacctccg ttcgggccca atcccgccct gcggaatccc ggccgcccaa ccccctgggg
      421 cgcgttccca ttctggaata ccatctgatc gcggagcagg acagccgctg gggccgctcc
      481 cgggacggct tccgccgcga tctcgagctg ctctatcaac gaggttaccg ccccgtcacg
      541 gtttccgagc ttatggatcg ccaagtcgac ttgccggcgg gcacgagtcc ggtcgttgtt
      601 accttcgacg acgcttcgcc cgggcagttc cggtacctcg agcgcgatgg acggctcgat
      661 atcgatcccg agagcgccgt cggcatctgg ctcgacttca ccgcgcggca ctccgattgg
      721 agaccccggg caacattctg tctcttgccc gcagccagcg cgggacatgc tttcttcggc
      781 gagaagaaca tcgaggggca gaagaccgcg tggcgctttc ccaagctccg tttcctgcag
      841 gagaaggggt tcgagctatg cgctcatacc ttgtggcacg cgaatctttc cactctctcc
      901 gacgccggcg tgcaggaaca gatcgcgcgc agtctgatcg ccattgactc tgcggtcccc
      961 ggacttcggg ttcgcacctt cgcccttccc ctgggcatct ggcccaagaa caaggccctg
     1021 gcccatgaag ggacctggcg ggatcccaag accggacggg tcgtgaacta ccggttcgac
     1081 gccatcctcg aggtgtcggg cggggctgcc cggagcccgg cggcgccgga gttcgacccg
     1141 ctgcgcttgc cccgtatcca ggtcatcggc gacaatctcg cccgcgagct cgaccggctg
     1201 gaaaaggatc gttacgtcgc cgccggtagc ggatccaccc actagcggac ggctcgtctc
     1261 tctcgacgtc ttccgcggcc tgaccgtggc ggcgatgctg ctcgtgaatg atccgggcag
     1321 ctggggtcac atctaccctc cgctgcgcca tgcggcgtgg cacggctgga cgccaacaga
     1381 tctgatcttt cccttcttcc tgttcatcgt cggagtcacc actcatttgt cgctcggcgc
     1441 gcgccgagcg cgaggagccg acgagaacca actggccgca caggtgctgc gccgcggcgc
     1501 catcatcatc gtgctggggc tgctgctctc ggccttccca tttttcccgc tggagcgtat
     1561 tgccgagatt cggattcctg gggtgcttca gcggatcggc ctggtgtatc tggcgaccgg
     1621 actgctggca ctccgtacca cggtccgcag cgagatcgcc atcgctgtga tcatcctggt
     1681 cgggtacaac ctgctccttg tggtcgtccc ggttcccggg gtgggccatc ccgcattagc
     1741 tccgccggac tcgacgctcg ctgcctgggt cgatcggcgc gtcctcgatg gacatctgtg
     1801 ggccgtaacc aggacctggg acccggaggg cctgttaccc acgatcccgg ccatcgcgac
     1861 ttgtattctc gggctgcttg ccggccgctg gatcacccgg tccggtccgc gggaggggcg
     1921 gataggcggg ttgcttgcgg cgggaggagc gggcatcacc gccggctggg tctggaacgc
     1981 ggcacttccc atcaacaaga acctgtggac cagctcgtat gtggtcttca ccgccggcct
     2041 ggcctgcgtg tgcctcgcgc tgtgcatgtg gctggtcgat gggcgaggac ctcgctggtg
     2101 gacccggccc cttgtggtat tcggtatcaa cccgatcgtc gcgttcgtcg gggcggaagc
     2161 attggcgcgg ctgctctatt cggtcatccg ggtgagctac gacggggaac cagtcccgct
     2221 ccaggtcgcc atctatcgat cgctctttgc ctcctggccg tcgccggagg ccggctccct
     2281 cggattcgcg ttgctgatcg tgctgttctg gtacggagtg ttggaagtgc tctaccggcg
     2341 aggcctggtc atcaggattt gagagcgatg cgccgccggc cggtacggtt ggaggcatgc
     2401 cagcgctcga gcgcggcgcc aagggtgctt accacgtaac gatgggcttc ggccatgagc
     2461 tccagacgct ggcggatcac cgggttgccg cggcgcagcc cttggcgggc ctctccgatt
     2521 aggcggctga accgacgcat ccgctcgaga cggagctcca gcgtcggaac cggcaagccg
     2581 ggcgctaccc ggtaatacac ccgccgatcc ccggcctggc cggtgcgttc cagcgctccc
     2641 cgccgctcga gcatgcgggt ctcggtgctg atgctcgcct tgctgacgcc gagatccgca
     2701 gcaagttggt cgagcgagag agacttttcg ctcaggagta atcggccgaa gatccgacct
     2761 gccgtccggg aaaagccctc agcctcgaag aacacaccga cctgctcgac gaactccgcc
     2821 ccatctgtca tgctagaatg caggaaggcc tccactgtat ggatgggtaa gcttacgttc
     2881 attcagtacg ttcaggcaat actgaatctt gaaagtgagt gtttccgtaa ggctcccgcc
     2941 ttcgcgtcaa ggagatcgcg ccctgccgat gccccgtctg attggctgtc atgtcgccag
     3001 cgcctatgcc gtcgtccttc tcctggccag ctgcgctccg ccgttgcccg ggcccggcgg
     3061 gggtgctccg gcggtggctc cggccccgca catcaaatgg acgccacccc gggaagcgcc
     3121 cgcggagcaa gcgcagccag cgcccgccat cccccccgat atcgctgagc gcatccagcg
     3181 actcaccatg ggcgacgtgg tggatcttgc gcttcggggc aacccggcaa ccgcgcaggc
     3241 gtgggccaat gcgcgagccg ccgccgctgc ctacggagcg tccaggtcgg catactatcc
     3301 caccctggac gccgactatt cggtcactcg gatcaagact gcagcgacca gcggacgtgt
     3361 ggcggtatcg caaactgtat acggccccag tgtcaccctg tcgtggctgc tctgggactt
     3421 cggaggtcgg agcggatcga tcgagtccgc tcgccagggc ctgcttgcgg cagactggac
     3481 ccataacgcg actatcgccg atgtcgtgct tcaggcgcag gtcgcgttct tcaactacat
     3541 cgcgaccggc gcgttcctca ccgcgcaacg agcgtccgtc aaggatgcag aggcaaacct
     3601 ggaggccgcc gacgcgcggc gacaagtcgg tgcggcgacc atcgcggacg tgttacaggc
     3661 gcggaccgcc ctgtcccagg cacggctcgc gctcgatacg ctggaaggca gtcggctgac
     3721 cgctcgcggg gcgctggctg tctcgctcgg cttgagcgcg aatgtcccgt acgacgtgga
     3781 cagcgtgcga gcgctgagac ccatcgccgg cgtctccgac agcgtcgaag cgatcatcaa
     3841 ccgggcggtc cgcgaccggc ccgacctggc cgctgcgcgt gccgaggcag aagctgcccg
     3901 agcccggatt cgcgagattc gcggcagtcg actgccgtcc atcaccgcga cggggacggc
     3961 ggcgcacacg gatgtcgccg gtcggggcag cgcgggcaac agctacaaca tcggcgtggg
     4021 gctggcggta ccgttgttca acggcttcgg ccgggaatat tcgcaaaagc aagccgagta
     4081 tctggccgac gccgcggcag ctcagcaacg tagcctggaa cagcaggtca cctaccaggt
     4141 gttcagcgcg tattatgcac tgcagacggc gacgcggcgg gtgcagacca gcaacgatct
     4201 ggttgcgagc gcgtcgcaat cgactgaggt cgccctcgcc cgctacaagg ctggcgtcgg
     4261 aaccgtgctc gacctgctcg cggcccaggg cgccttggcg gcggccaggt cgcaggtagt
     4321 ccagggccgc ctggcctggt acatctcgct cgcgcagctt gcgcacgacg caggcgccct
     4381 cgacccgaga ggtggaaccg acctccggat gtcgcccgat accgtggaga gcattccacg
     4441 tcaatgaagc gaacgttctt gctgtttctg accgccttcg ccgctctttc gagcgcttgc
     4501 aacaggacgc cccggcggga gctgccgccc gtaccggtgg tcgccgccac cgccgagcgg
     4561 cgctccgtgc cgttcaccct ggaagcgaat gggacggtcg agccactgga gacggtggcg
     4621 gtcgaatcac aggtgaccgg ctatctgctt cgagtcggct tcaaggaagg tgacgaggtt
     4681 cgcccgggac aggtgctgtt cgagatcgat cccgcgccgt atcgcgcggc attggaccag
     4741 gcgcgggccg tgctggcgag ggatcaggcg cagcttaccg cggcgaagca ggacgcgcag
     4801 cggtatgagc agctggctgc gaaggagtac gtgacgaccc agcaggcgga gcaggcgcgc
     4861 gccactgccg gggcccaggc ggcctcagtc aaggccgatg aagccgccgt ggaaagcgct
     4921 cggatcaacc gggacaacgc cacgattcgg tcgcctatcg gcggcaaagc gggcggcctg
     4981 ctggtcaagc cggggaacct ggtgcgcggg ggggcaggcg ttcctctggt cacgatcaat
     5041 cgtattcatc cgatcctgg
//
LOCUS       DASRJQ010000253         4471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1937589, whole genome shotgun sequence.
ACCESSION   DASRJQ010000253 DASRJQ010000000
VERSION     DASRJQ010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1937589"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..1065
                     /locus_tag="VFB89_05735"
     CDS             202..1065
                     /locus_tag="VFB89_05735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HZM26835.1"
                     /translation="MPRPRALDQILRLDPERDHQRICFMSACVEFPWDTTRSLQLALF
                     RTYCVPRMTELLVSTGEFARHAQKRYDDTVLILSELLESGYDSERGRAAQRIMNRLHG
                     LCPIRNEDYLYVLSTFVYEPIRWNARFGWRSLTEQEKLAGFHFWREIGRRMNIRAIPE
                     SFGAFERYNVEYERREFRYADSNRVIGDATKALFASWFPAPLGPLVDAAIYALLDEPV
                     LDAFGYPHPSPVTRRCVEGALRLRAQVVRHLPRRRRPRLLTATRHRSYPRGYRVDQLG
                     AAAAHTSPSAR"
     gene            complement(1404..1610)
                     /locus_tag="VFB89_05740"
     CDS             complement(1404..1610)
                     /locus_tag="VFB89_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26836.1"
                     /translation="MNGAPHRGDHLDAILATAGFATHLVSDSVSARGVLEIWRPSLVV
                     VDFRPPAGETRQFCTDLATYASTR"
     gene            complement(1706..2437)
                     /locus_tag="VFB89_05745"
     CDS             complement(1706..2437)
                     /locus_tag="VFB89_05745"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HZM26837.1"
                     /translation="MLQIRRDREIYEIEGGWFHARWHFSFDQYQDPENDSFGALRVFN
                     DDRLIPGAVWPLHPHRDVEGLTYVVEGTFGHKDNRGGPFGPLSAGSVQRMSLGSGALH
                     SEMNASKTEPMRFIQIWILPDTAGLKPEVEQRVFTVADRTNRLLKVIGPEGDDVVKVH
                     QDATVYVASLAPGIGVTHEIGAGRGVYAYLIGGAASFDDQDVETGDAAKVTNQPSLQI
                     LARDQSELILVDVPMTFDAVGTWKG"
     gene            complement(2738..2863)
                     /locus_tag="VFB89_05750"
     CDS             complement(2738..2863)
                     /locus_tag="VFB89_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26838.1"
                     /translation="MLHTLRRDRVYELLDIAGLTGVTQARREALLALGAVDWTRT"
     gene            complement(2887..3159)
                     /locus_tag="VFB89_05755"
     CDS             complement(2887..3159)
                     /locus_tag="VFB89_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26839.1"
                     /translation="MLDAQTGPEWGMAISRDGRLLASGGVAGVITLWDVPMRSARAVL
                     TGHTSPIWGLALSSDGRLLASASFDATARLWDTRSGDCLGVLTGQL"
     gene            complement(3466..3759)
                     /locus_tag="VFB89_05760"
     CDS             complement(3466..3759)
                     /locus_tag="VFB89_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26840.1"
                     /translation="MTESLARKALKRGMKTWIVTSDKVQMMIGLFREELEDLLAEART
                     EYEQQATTGAQADADEQTAAVITAQAAHPTRRRAKRGARGKDSDPAQETTTST"
BASE COUNT          748 a         1487 c         1431 g          805 t
ORIGIN      
        1 tcgagctccg ccgggaggcg cgccgccagc aaggacgctt ccgcaagaac ccgacggcct
       61 acctggccgc gctggaggcc aggctactca agccgagttt gccgtcctag tttttttgcc
      121 ctcgacatcc tggcgatgac caatcatcgc gttggccgga tcgcgttcgc gggcagtacg
      181 atcacgtgtt caggaggcga tatgcctcgc ccacgtgccc tggaccagat cctccgcctc
      241 gaccccgaac gcgaccacca gcggatctgc ttcatgagcg cctgcgttga gttcccttgg
      301 gacacgaccc gctcgctgca actggccctc ttccgcacct actgcgtgcc gaggatgacc
      361 gagctcctgg tcagtactgg ggagttcgcg aggcacgccc agaagcggta cgacgatacg
      421 gtgctgatcc tctccgagct gctcgagtcc ggctacgaca gcgaacgggg gcgcgccgcc
      481 cagcggatca tgaaccggct gcacgggctg tgcccgatcc gcaacgagga ctacctgtac
      541 gtgctgtcga ccttcgtcta cgagcccatc cgctggaacg cgcgcttcgg ctggcgctct
      601 ctgactgagc aggagaagct ggccggcttc cacttctggc gagaaatcgg ccggcggatg
      661 aacatccgcg cgatccccga gagcttcggg gcattcgagc gctacaacgt ggagtacgag
      721 cggcgggagt tccggtacgc cgacagcaac cgcgtcatcg gcgacgcgac gaaggcgctg
      781 ttcgccagct ggttcccggc gccgctgggc ccgctggtcg acgcggcgat ctacgcgcta
      841 ctcgacgagc cggtcctcga cgcgttcggc tatccccacc ccagcccggt cacgcggcgc
      901 tgcgtcgagg gcgcgctgag gctacgcgcc caggtggtgc gccacctgcc ccgccgacgc
      961 cgcccgcggc tgctgaccgc gacgcgccac cgcagctacc cccgcgggta tcgcgtcgat
     1021 cagctggggg ccgcggcggc gcacacgtcg ccatccgctc gctagcctgg cgagccgacg
     1081 tcgccggcct gggcatcttt gacggagccg cgcccgtcga gctccggacg agacggtggc
     1141 ctgcttcatg cggggttcgt gtgggcgtgg ctatcggggc tagaccacgg gtatctggac
     1201 caggtcgcgg gcgccgccca gactgggtga gatttcagga tggccgtggt cgcatgtgcg
     1261 cgggcttcgc atgcgattca agctggtggc agcagaaaca gcaagccgga gctcgtgatg
     1321 agatgtcacg tacgaaggga cggcgccaga cgattgccgc tcacgtaact ggaggccctt
     1381 ccccgacgag tatgaccggc agatcagcga gtgcttgcgt acgtagccag gtcggtgcag
     1441 aactggcggg tctcacctgc cggaggccga aaatcgacga ccaccagcga tgggcgccag
     1501 atctcgagga cgccacgtgc cgacacgctg tccgagacca ggtgggtcgc gaacccggct
     1561 gtcgcgagga tcgcatcgag gtgatcgcct cggtgcggag ctccgttcat aatcaagacg
     1621 aggtcgcgcc gaagctggta cgggctgccg gccgagcaga ctcatggccg cctgatcgct
     1681 cccggcgcta cacggctgga tggacctatc ctttccaggt gcccaccgcg tcgaacgtca
     1741 tcggcacgtc gacgaggatc agttcgctct ggtcgcgggc caggatctga aggctgggct
     1801 ggttcgtcac ctttgcggcg tccccggttt cgacatcctg gtcatcgaac gaggcggcgc
     1861 cgccgatcag atacgcgtat acgccacgtc cggcgccgat ctcgtgggtg accccaatcc
     1921 cgggtgcgag cgacgccacg tagacggtgg cgtcctggtg caccttgacg acatcgtcac
     1981 cctctggccc gatcaccttg agtagcctgt tggtccggtc cgcgacggtg aagacccgtt
     2041 gctccacctc gggcttcagc ccggccgtgt cgggaaggat ccagatctgg atgaagcgca
     2101 tcggctcggt cttcgaggcg ttcatctccg aatgcagagc tccggacccc agactcatgc
     2161 gctggaccga gccggccgac agcgggccga acgggccacc gcgattgtcc ttgtggccga
     2221 atgtgccttc gacgacgtag gtcagcccct caacgtcgcg atgcgggtgg agaggccaga
     2281 ccgcgcccgg gatcagccgg tcgtcgttga agacccgcag cgcaccgaag ctgtcgttct
     2341 cagggtcttg atactggtcg aacgagaagt gccagcgcgc gtggaaccaa ccaccctcga
     2401 tctcatagat ctcacggtca cgacggatct gcagcatggt ggactcctgt taccgagtac
     2461 ctgatcgtct tgcggcacgg gcagggctac tggactgtcg ccttggccgg gggtgggcgt
     2521 agccgcccta cgtcgccgcc agcggccagt ccgggttgac cggcagcagc ggtgggccga
     2581 gcgtctagcg atcaccggcc ccctgtactg ccatcctggc atcgtccaga tcgcgcgtcg
     2641 cccacccacg gtctgatgcc aaccgtacct ttggtggaga cgatcgcacg ttcgcgaggt
     2701 gcgacgagct gctgttcacg gatccggtca ggccacgcta cgttcgtgtc cagtccaccg
     2761 ctccaagagc gagcagcgct tcacgccggg cttgagtgac gccggtgagt ccggcaatat
     2821 ccaggagctc atagacgcgg tcccgcctca gcgtgtggag catgctttca tgctccacat
     2881 cccagatcac aattgtcccg tcaagacccc caggcagtct ccgctgcgcg tgtcccacag
     2941 gcgggcggtc gcgtcaaagc tggcgctggc aaggagccgt ccatcgctgc tcagggccag
     3001 cccccagatt ggactggtgt gacctgtgag caccgcgcgt gcactccgca tcggcacatc
     3061 ccacagtgtg atgacgcccg cgactccacc actcgcaagc aagcgtccat cgcgactgat
     3121 ggccatcccc cactcaggac cggtctgtgc atccagcacc gcgacgacat tgccggtttc
     3181 gagtcgatcc ccggcttcgg ttagcgcaac gctccacgcg ccgctggtgt ggccgggcag
     3241 cgtcgcgatg agttgtccag tatcaacctg ccagacacgg acgtgacatc cacagttcag
     3301 gaggtggccg gccaggccgc gcgttcattc gcgcagttcg cacgggacta tgcacccgca
     3361 ttctcctaaa cgcaggggcc gccccgatcg tcgatcccca gaagctcccg ctggcgagcg
     3421 cacctcagcc gtgcgtgtga ttggacactt gctttctttg ggagtctatg ttgaagtcgt
     3481 cgtctcctgt gctgggtccg agtccttacc acgcgcgccg cgcttcgctc gacgacgggt
     3541 ggggtgtgct gcttgtgccg ttatgaccgc cgcagtctgc tcatcagcgt cagcctgagc
     3601 accggtcgtg gcctgctgct catactcggt ccgcgcctcg gccagcagat cctcgagctc
     3661 ctctcggaat agaccgatca tcatctggac cttatcgctc gtcacgatcc aggtcttcat
     3721 tccgcgcttc agagcttttc gcgccaggct ctcagtcatg tgagtctccg gggcattgtg
     3781 gtgttgtggt gtgacggctg agcaagagag atcgtgcctc gcgatacacc agaatcggga
     3841 tgaacgaggt gacttgcgcg tccttgtaac gcgctgcgca ctgttcgacg atccgttgaa
     3901 tctgcgcacg agaatgcagt gattccagct ccgagacgag ccgctcgatc tgcgtatcga
     3961 gggctgcgtc aagctaggac gctgccatga agtcctccag agtcccgatc acgggaataa
     4021 tgctttcgat tgggacggat ccacgccacg cgtcgcacac gcgatggctg cctctcgccc
     4081 ggtggcggcc tccggattgt cgttgtaagg gcggcaggtg aaatccaaaa gactcgacgc
     4141 gccagcgcgc tcacggcggc gcggatggtg cgagaacccc gagggatcac tctacggctg
     4201 acgtctcgag gctgcatgct ccatagtggc gtaccttcgg cccagcgtgg cccgggttgt
     4261 cgcggggctg ccagtgcccg atcaccctcg ccagcgatcc agttgagaca aagcctgaag
     4321 gcaggccggt ctacgtgatg gtcaaggagg ccaatcggcg gatgatcgcc cccggtcagg
     4381 acaagccggt caaggagccg tcgctcccag gccgctcaga gggagtcgcc tctctatggg
     4441 tcgattctgc cgacgcctcg accggtctca g
//
LOCUS       DASRJQ010000254         3581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1251404, whole genome shotgun sequence.
ACCESSION   DASRJQ010000254 DASRJQ010000000
VERSION     DASRJQ010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251404"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="VFB89_05765"
     CDS             complement(<1..1100)
                     /locus_tag="VFB89_05765"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HZM26841.1"
                     /translation="MATSKTTRAKPASKPTAAEARKARPKAAKSSGNGAGKRQSRRAG
                     EALVIVESPTKAKTIGRYLGNGYAVKATIGHVRDLPTRKLGVEIDKGRFTPEYVIIKG
                     KNQTLIEIKKAAKGASEVYLATDPDREGEAIAWHVASQLDRAVPTHRVLFHEITKDAI
                     REAMQNPGDIDEQKVNAQQARRILDRLVGYKASPLLWRAVKTGLSAGRVQTVALRLMV
                     EREREIRAFRSQEYWTIEAECAKDGQSFEASLHKVDGHKPQLHSEAEAKTVVETVRGL
                     PFVVSRVERKQRRKRPAAPFTTSTLQQEAAKRLGFSSRRTMRAAQDLYEGVDLGEEGP
                     IGLITYMRTDSVRVSDTAISSVRAFIKKNYAK"
     gene            complement(1215..1739)
                     /locus_tag="VFB89_05770"
     CDS             complement(1215..1739)
                     /locus_tag="VFB89_05770"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HZM26842.1"
                     /translation="MSRKHLLLIGLPGSGKSTIGRMVAELLNAPLIDVDNLLVRQMGM
                     PITQIFGMVGETEFRRMERDAVRAACVAEPVVLVPGGGWAAQEGELDAVREATCIVYL
                     KCQPGTAAKRAEQGEVRPLLTAEDPVARMRTLLQEREPYYNKAEHSVITDNKAADAVA
                     AEVAQVARQQAGWL"
     gene            complement(1736..2986)
                     /gene="aroC"
                     /locus_tag="VFB89_05775"
     CDS             complement(1736..2986)
                     /gene="aroC"
                     /locus_tag="VFB89_05775"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HZM26843.1"
                     /translation="MQLRFVTAGESHGKALVAVVEGLPAGLPVTAEWVDRDLARRMMG
                     YGRGARMKIERDQVEFLSGVRSGETLGTPVAMLVRNRDWENWADVMAAEGAPGDLRRR
                     RVTRPRPGHADLAGVLKYDRLDARDILERASARETVARVAAGALARRLLDEFGVDIGS
                     HVVSLGGVRATLPAELPRPLNDAADRSEIRVLDPAVEAEIIARIDAAKKDGDTLGGEV
                     EVVARGLVIGLGSHVSWDRKLDGRLAGMLMSIPAVKGVEIGLGFEAARRLGSAVHDPI
                     DAEGQSSASARAMPPHPGRPRAGFSRRSNNAGGIEGGMTTGEAVVIRAAMKPISTLMT
                     PLPTVDLVTGEAANAQSERSDVTAVPAMGVIAEALVALVLADAMLEKFGGDSLGEMKR
                     NFKAYVGALGARWTQLRAEGAEPR"
     gene            complement(3155..>3581)
                     /locus_tag="VFB89_05780"
     CDS             complement(3155..>3581)
                     /locus_tag="VFB89_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HZM26844.1"
                     /translation="GEETPVPGAPPAQGSAVAVQQTQFKETGIRLTVTPHVTNSGQIL
                     MTMRTERSAVQTLPASQFGFNFTKQTTENQMLVNDGETAVIGGLTITEVNRTRSGIPL
                     LSQLPLVGGLFSTTERSERRRDLMILVTPRVVDDNGSLN"
BASE COUNT          562 a         1263 c         1069 g          687 t
ORIGIN      
        1 tcctttgcgt aattcttctt aatgaaagcg cggaccgagg aaatggccgt gtccgagaca
       61 cgcacagagt cggttcgcat gtacgtaata aggccgatcg gcccttcctc tccgagatcc
      121 accccttcgt agagatcctg cgctgcgcgc atcgtgcggc gggaggagaa gccgaggcgc
      181 ttagcggcct cctgctgtag agtgctggtc gtgaagggcg cggccggccg cttgcggcgt
      241 tgtttccgct ccacccggct gaccacgaac ggcagtcccc ggaccgtctc cacgacggtc
      301 tttgcctcgg cctccgaatg gagctgaggc ttgtggccat ccactttgtg aagagacgcc
      361 tcgaagcttt ggccgtcctt ggcgcactcc gcctcgatgg tccagtactc ctgggacctg
      421 aacgcgcgaa tctcgcgttc gcgctcgacc atgagccgga gagccacggt ctgcaccctg
      481 ccggctgaga gacccgtctt gacggctctc cacaagagag gacttgcctt gtatcccacg
      541 agccggtcga gaatgcgccg ggcctgctga gcattcacct tctgctcgtc gatgtctccc
      601 gggttctgca tcgcttccct gattgcgtcc ttggtgatct catggaacag gacgcgatgg
      661 gtaggcaccg cgcgatccag ctgacttgcg acgtgccacg ctatcgcctc accctcacga
      721 tcagggtcag tggcgagata gacttcgctc gcgccttttg ctgccttctt tatctcgatg
      781 agcgtctggt tcttgccctt gatgatcacg tactcgggcg taaaacggcc cttgtcgatc
      841 tcgacgccaa gctttctcgt gggcagatcg cggacgtggc cgatcgtcgc cttcacggcg
      901 tacccgttcc cgagatacct gccgatcgtc ttggctttgg tgggagattc gacgatcacg
      961 agggcttcgc ccgcccgacg tgactgtcgt ttcccagcgc cgttgccgct gctcttggcg
     1021 gcctttgggc gcgccttccg cgcttcggcc gcggtcggct tggatgctgg tttggctctg
     1081 gtggtcttgc tcgttgccat acgcttcagg gtaccgattc ctttcatccc cgggtcaacc
     1141 ggggcccggt taagaaaccc gcacggttcc cggatcgcaa ggacaagctt ctcaacgctc
     1201 gatgtttcag tgagttacag ccaaccggcc tgctggcgag caacctgagc cacctcggcg
     1261 gcaaccgcgt cagctgcctt attgtcggtg atgaccgagt gctcggcctt gttgtagtag
     1321 ggctctcgct cttgcagcag tgtgcgcatc cgagccacgg gatcttccgc cgtgaggagc
     1381 ggccggacct ccccctgctc cgcccgtttg gcggcggtgc ccggctgaca cttgagatag
     1441 acgatgcagg tcgcttcgcg cacggcgtcc agctcgcctt cctgggcggc ccagccgccg
     1501 cccggtacca gcacaaccgg ctccgccacg caagcagcac gcacggcatc ccgctccatc
     1561 cggcggaact ccgtctcccc gaccatcccg aagatctggg tgatgggcat gcccatctga
     1621 cggaccagga gattgtccac gtcgatgagc ggcgcgttga gaagctccgc caccattcgg
     1681 ccgatggtgc tcttgccgga tcccggcaac ccgatcagga gcagatgctt tcgactcacc
     1741 tgggctccgc cccctcggcc ctgagctgcg tccaacgagc gcccaaggct ccgacatagg
     1801 ctttgaagtt ccgcttcatc tcgcccagac tatcgcctcc gaacttttcc agcatcgcat
     1861 cagccagcac cagcgccacg agcgcttcgg cgatcacacc catcgccggt acagccgtga
     1921 catcagaccg ctcactctgt gcgttggcgg cctcacctgt caccagatcg accgtcggaa
     1981 gcggcgtcat cagagtcgag atcggcttca ttgccgcgcg aatgaccacc gcctcacccg
     2041 tggtcatgcc accttcgatg ccaccggcat tgttgctgcg ccgggagaat ccggcgcgcg
     2101 gcctgccggg atgcggcggc atcgcgcgcg cagacgcact cgactgacct tccgcatcga
     2161 tcggatcgtg caccgcggac cccagtcgcc gggctgcttc gaagccgagg ccgatctcga
     2221 cgcctttgac ggcggggatc gacatcagca tgcctgcgag ccgtccgtcg agcttgcgat
     2281 cccagctcac atggctcccc agcccgatga ccaaaccccg ggctacgacc tcgacttcgc
     2341 cacccagcgt gtcgccgtct ttcttggctg catcgatgcg agcgatgatt tccgcttcga
     2401 ctgccggatc gagcacccga atctcggacc ggtccgccgc gtcgttgagg ggccgcggca
     2461 gctcggccgg cagcgttgcg cgcacgcctc ccagcgacac tacgtgactg ccgatgtcga
     2521 cgccgaactc gtccagcagc cggcgggcca gtgcgcccgc cgcgacgcga gctaccgtct
     2581 cgcgcgcgct ggcgcgctcg aggatgtcgc gggcatcgag tcggtcgtac ttgagcacgc
     2641 cggcgagatc cgcatgcccc ggtcgaggac gagtcacccg gcgcctccgc aggtcgcccg
     2701 gagcgccctc tgcggccatc acgtctgccc agttctccca atcccggttc cggacgagca
     2761 tcgcgaccgg cgtacccagc gtctccccgc tccggacgcc ggagaggaat tccacctggt
     2821 cgcgctcgat cttcatccgg gcgccgcggc cataccccat catccgccgg gccagatcgc
     2881 ggtccaccca ttctgctgtc accggcaggc cggccggcag cccttcgaca acggcgacga
     2941 gggccttccc gtgcgattcg ccggcggtaa cgaaccggag ctgcagacaa gactcctgtc
     3001 aggtgaacgg tgaacagtga acggtaagcg ggccaccccg ctaccccccc gttcaccgtt
     3061 cactgttcac cgttcaccgt ttttaagcaa tcgagcccga cacgctggtc gggctcgatt
     3121 ctactcctcc gggcaccgcc cggccaagag cctgctagtt caagctgccg ttgtcatcca
     3181 ccacccgtgg cgtcaccagg atcatgagat cgcgacggcg ctccgagcgc tcggtcgtac
     3241 tgaacagccc tcccaccagc ggcaactgcg acagcagcgg gatgcccgac cgggtccggt
     3301 tgacctcggt gatcgtcaga ccgccgataa ccgcggtctc gccgtcgttc acgagcatct
     3361 ggttctcggt cgtctgcttc gtgaagttga agccgaactg ggaagccggc agcgtctgca
     3421 ccgcggaccg ctcggtgcgc atcgtcatca ggatctgacc gctgttggtc acgtggggcg
     3481 tgaccgtgag gcggataccg gtctccttga actgggtctg ctgcaccgcc actgccgagc
     3541 cctgcgcggg cggcgcaccc ggcaccggcg tttcttcacc g
//
LOCUS       DASRJQ010000255         2474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3539611, whole genome shotgun sequence.
ACCESSION   DASRJQ010000255 DASRJQ010000000
VERSION     DASRJQ010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3539611"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2342)
                     /locus_tag="VFB89_05785"
     CDS             complement(<1..2342)
                     /locus_tag="VFB89_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM26845.1"
                     /translation="MSLPPGMRLGPYEIVAPLGAGGMGEVYCARDTKLGREAAIKVLP
                     AESVHDDTARARLLREAQLAATLNHPNICTVYEVGEADGRIYIAMELIDGLPLSEKIA
                     ERVTGLATETVVRYGVQIAAALAHAHEQHVIHRDLKSSNVVVTPEGRVKVLDFGLARR
                     ASEPPEEGGATLSMALTETGAVVGTPHYLAPEILRGRVANVRSDLWALGVLLHEMASG
                     SLPFEGTTRFELASAILHDVPAALPDRVPPGLRAVVGRCLAKEPGERYQRASEVRAAL
                     ETLQSGESRTAAAPAMRASRVLPVAMTVLLLAAAIVAIGYVLRQSAAPHDFKQRQVTS
                     NPAGDPVGRGDISPDGKTLAVVDRSGLSLRSIESGESHPMVLPAGFTFDYPFPQISWF
                     PDGSQLLVSGRIAGGPSGVWALPVAGGRTRKVLDNASFGVLSPDGTHIAYKQRGARGH
                     EIWVCRANGEGASRVTGDDSSGVIPAWAVWSPSGKRLAYARGDIGTETVRLESSDLAG
                     RTRVLYLPTTNQALHYFTIPAWLPDGRVVFGLTDPPPNQRDMNLWSLRVDPNSGMPAG
                     STRRVTQWQHLAHVGPTAYSADGRRLTVAALEYQSDVYVGRMGVGRAQLLDVRRVTLD
                     DRMDISATWMPDDSTILFCSDRNGTFDIFRQRIDTAGAEPVVTGRGDQYSPQLSPDRA
                     WILYAEQDAGDLIPTSTNARLMRIPLNGGRAEKVLDAQGTATFRSPQATRTSPVLCEL
                     RSGSIVFSTFDPLRGRGRELSHVEGHAQPPWSLSPDGSTIA"
BASE COUNT          410 a          829 c          809 g          426 t
ORIGIN      
        1 gcgatcgtcg agccgtcggg cgagagactc cacggtggct gagcgtgtcc ctccacgtgc
       61 gacagttccc ggccacggcc gcgcaacgga tcgaaagtcg agaacacaat ggagccactc
      121 cgaagctcgc agagcactgg ggaagtccgg gtcgcctgcg gagatcggaa cgttgccgtg
      181 ccttgggcgt cgagcacctt ttcggcgcgg ccgccgttga gcggaatccg catgagacgc
      241 gcattcgtcg acgttggaat caggtctccg gcgtcctgct ccgcgtagag tatccacgcc
      301 cggtctggac tgagttgcgg agagtactgg tctccccgac ccgtcacgac tggctctgcg
      361 ccagcagtgt cgattcgctg ccggaagatg tcgaacgtgc cattgcgatc tgaacagaac
      421 aagatcgtcg aatcgtcggg catccaggtt gcgctgatgt ccatgcgatc gtcgagcgtt
      481 acacgccgga catcgagaag ttgtgccctc ccgacgccca tgcgtcccac gtacacatca
      541 ctctggtatt ccagcgcagc gaccgtcagc cgcctaccgt ctgcggagta tgcggtgggc
      601 ccgacatggg caagatgctg ccattgggtg acgcgccgcg tgcttccggc tggcattcct
      661 gagttcggat caacacgcag cgaccacagg ttcatgtcgc gctggttggg agggggatcg
      721 gtgaggccga agaccacgcg cccgtcgggt agccacgcag ggatcgtgaa gtagtgcagg
      781 gcctggttgg tcgttggtaa gtagaggacg cgagtccgcc cggccaggtc ggagctttcg
      841 aggcgtacag tctccgttcc aatgtcgccg cgggcatagg ccagccgttt cccgctcggc
      901 gaccaaactg cccacgctgg aatcactccg ctggaatcgt cgccggtcac gcgcgaggca
      961 ccttctccat tcgccctgca cacccatatc tcatgacctc gcgcaccccg ctgcttgtag
     1021 gcgatgtgcg tgccatctgg tgagagaacc ccgaacgacg cattgtcgag caccttgcgc
     1081 gtgcggccgc cggccacggg cagcgcccac acgcctgacg gtccgcccgc tatgcgaccg
     1141 ctgacgagga gttgactccc atccgggaac cagctgatct ggggaaacgg atagtcgaag
     1201 gtgaatcctg ccggaagcac catgggatgg ctctcgccgc tctcgatgga acgaagggag
     1261 agaccgctac gatccacgac cgccagggtc ttgccgtcgg gcgagatgtc gcccctcccc
     1321 accgggtcac cggcggggtt cgaggtcacc tgccgctgct tgaagtcgtg aggagccgcc
     1381 gactgacgga gcacgtaccc gatcgccacg atcgctgcgg ccagcagtag caccgtcatc
     1441 gcgaccggca gcactcgaga agccctcatt gcgggagccg cggcggttct tgattctccc
     1501 gattggaggg tttcgagcgc cgcgcgcacc tcgctggctc gctggtagcg ctccccgggc
     1561 tcctttgcca ggcatcgccc gacgacggcc cggagccctg gaggcacgcg atcgggcaat
     1621 gctgcgggga catcatgcaa gatcgccgac gccagctcga agcgggtggt gccctcgaac
     1681 ggcaggctcc cgctcgccat ctcgtggagg agcaccccga gcgcccacag gtcgctccgc
     1741 acgttcgcca ctctcccgcg caatatctcc ggcgcgagat agtgcggcgt cccgacgacc
     1801 gcgccggtct cggtgagcgc catgctcaac gtggcgcccc cttcctcggg tggctcggag
     1861 gcacgccgtg cgaggccgaa gtccagcacc ttgacccggc cctccggggt gaccaccacg
     1921 ttcgaactct tgaggtcgcg gtggatcacg tgctgctcgt gcgcgtgtgc cagcgccgcc
     1981 gcgatctgca ccccgtagcg caccacggtc tcggtcgcca gcccggtgac ccgctcggcg
     2041 atcttctcgc tcagcggcag gccgtcaatc agctccatcg cgatgtagat gcgcccgtcg
     2101 gcctcgccta cttcgtacac ggtgcaaatg ttcgggtggt tgagggtcgc cgcgagttgc
     2161 gcttcacgca gcagccgggc acgggcggtg tcgtcgtgca cggactcggc gggcaacacc
     2221 ttgatggccg cttcgcgccc gagcttggtg tcgcgcgcgc agtacacctc acccattccg
     2281 ccggcgccga ggggggcgac aatctcgtag ggaccaaggc gcatgccggg gggcagggac
     2341 attggcggaa tctaccccac cgataagcag cgcgtgagat gccgcatcgc gtccgcggtg
     2401 actcagggtg tcatcagctg ctcgtagacg atttcgtacg gctcgggctt caagccgagg
     2461 tcaccgtcgc atgg
//
LOCUS       DASRJQ010000256         2507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1753505, whole genome shotgun sequence.
ACCESSION   DASRJQ010000256 DASRJQ010000000
VERSION     DASRJQ010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1753505"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..552)
                     /locus_tag="VFB89_05790"
     CDS             complement(61..552)
                     /locus_tag="VFB89_05790"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HZM26846.1"
                     /translation="MIQVVVDDLAFVTADAVVRPADEVLNPVSPETIRLDRQAGERFA
                     ALRRVTTPLDAGAAVVTGGGDLAAPFVLHLVIRDSSHRTDRDIVRRALISAWQRAADW
                     GLDSIAAPLIGAGAGQLTLEDAARLMVETFEAHRGGAELRIVLEREDDRALVEAIVQR
                     RPG"
     gene            complement(549..1268)
                     /locus_tag="VFB89_05795"
     CDS             complement(549..1268)
                     /locus_tag="VFB89_05795"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM26847.1"
                     /translation="MARTREQRATKPTRRRKKAAGPTPGVAEPISNAAAIDGPVAVVL
                     GPLPTATPASQHVVPAAHTNGNGNGRHNGVYGEEDADEDDERPDHVMALLDERIRWDP
                     DSVELLVRRGALLGRQLRLREAEADLRKALRLEPSHSQALLDLGLTLWRRGNAAEAAE
                     MLDRAAAHQPQNPTAFNYLAEALHQIGQAENAVVALERSLALDPDQPRAYHLMGRVLD
                     RLRRPEEARQAYRRGQELSRQ"
     gene            1400..>2507
                     /locus_tag="VFB89_05800"
     CDS             1400..>2507
                     /locus_tag="VFB89_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZM26848.1"
                     /translation="MAQEWLVVRGAREHNLRDISVRIPRNQLTVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGLMEKPDVDAIEGLSPAISIEQKSQGQNPRSTVGT
                     VTEVYDYLRLLWARVGIPHCPNDGSPVARQSASQITDAVLLWPEETRIEVLAPLVRGR
                     KGEFRELFEDVRKRGFVRLRVDGATYDVAEAPALNRRQNHDIAVVVDRLVVRAADRSR
                     LSDSIETALKTADGIVEVVRYNGERRETDSTVFSERFACPVCGLSFPELEPRQFSFNS
                     PFGACPSCHGVGTRREVNAELILGDLSISILEGVILPWGEPSGYLRKVVLPTLARAFK
                     FDLAAPWGELSEDARRAILYGAPGRFRFAVDGGKG"
BASE COUNT          392 a          817 c          836 g          462 t
ORIGIN      
        1 actcaatgcc gtccactgcc ttgaacttgc cgtaccgctt ggtcaaaccg gtcagggtga
       61 tcacccgggt ctccgctgca cgatggcttc gaccagtgct cgatcgtcct cgcgctccag
      121 tacgatccgg agctctgccc cgccccggtg cgcctcgaag gtctccacca tgagcctggc
      181 ggcgtcctcc agggtcagtt ggccggcgcc ggcccctatc agaggggccg caatagagtc
      241 caggccccag tcggccgccc gttgccaggc ggagatcagg gcgcggcgca ctatgtcgcg
      301 gtcggtgcgg tgactcgagt cgcggatgac caggtgcagc acgaacggtg ccgccaggtc
      361 gccgccaccc gttaccacgg cggcaccggc gtcgaggggg gtggtgaccc ggcggagggc
      421 ggcaaaacgc tccccggcct gccggtcgag ccggatggtc tcgggggaga ccgggttgag
      481 gacctcatcg gctggccgga cgaccgcgtc cgcggtaacg aacgcgagat cgtcgacgac
      541 cacctgaatc actgccggga aagctcctgt ccgcggcgat aggcctgtcg ggcctcctca
      601 gggcggcgca accggtccag caccctaccc atcaggtgat aggcacgggg ttgatccggg
      661 tcgagcgcga gagagcgctc gagcgcgacg acggcgtttt cggcctggcc gatctgatgg
      721 agcgcctcgg ccaggtaatt gaacgcggtt ggattctgcg gctggtgcgc cgctgcccgg
      781 tccagcatct cggccgcctc agccgcattc ccccggcgcc acaacgtgag gccgaggtcg
      841 agaagggcct gactatgcga cggctcgagt cgcagtgcct ttcgcaaatc ggcctcggcc
      901 tcccgcaggc gaagctgccg gccgagtaga gcgcctcgac gtaccagcag ctccaccgag
      961 tccggatccc agcggatccg ttcgtccaaa agcgccatca cgtggtccgg ccgctcgtcg
     1021 tcttcgtcgg catcctcctc accatacact ccgttgtgcc ggccgttgcc gttcccgttg
     1081 gtgtgggctg ccggcacgac atgctggctt gctggtgtgg cggtggggag tgggccgagg
     1141 acgaccgcga ctggaccgtc gatggcagca gcgttggata tcggctccgc gacccccggc
     1201 gtcggacccg ctgctttctt gcggcgcctg gtcggtttcg tcgcccgctg ctcacgagtt
     1261 cttgccatgc tgactccaga ctggatcgat tcttccatat gctacggctc gtttcgggaa
     1321 tgcgtaagac tggggccgcg tggtctcgaa cgcccgcgga ttgaagcgcg ccagggaccc
     1381 ccggtagctt tccccggcta tggctcagga atggctcgtc gttcgaggtg cccgggagca
     1441 caacctccgc gacatctcgg ttcgcatccc tcgtaatcag ctcacggtca tcaccgggct
     1501 gtccggctcg ggtaagtctt cgctcgcctt cgatacgatc tacgcggaag ggcagcgtcg
     1561 gtacgtcgag tcgctctcgg cctacgctcg ccaatttctc ggcctgatgg aaaagccgga
     1621 tgtcgacgcc attgaaggcc tgtcgcccgc catttcgata gagcaaaaga gtcaaggaca
     1681 gaatccgcgc tcgacggttg gaaccgtcac cgaggtctac gactatctgc gactgctctg
     1741 ggcgagagta ggcatccctc attgccccaa tgacggcagt ccggtcgcgc ggcagagtgc
     1801 ctctcagatc accgatgcgg tcctgttgtg gcctgaggaa acccgcatcg aggtcctggc
     1861 accgctggtt cgcgggcgca agggcgagtt ccgcgagctg ttcgaggacg tccgcaagcg
     1921 cgggtttgtt cgtctgcggg tcgacggggc gacgtacgac gtcgccgaag ctcccgcact
     1981 caaccgccgg cagaaccacg acattgcggt cgtagtcgac cggctggtcg ttagagccgc
     2041 ggaccgatcg cggctcagcg attcaatcga gacggcgctg aagacggccg atgggatcgt
     2101 ggaggtggtg cgatacaacg gggaaaggag agagactgac tccaccgtct tctcggagcg
     2161 ctttgcctgc cctgtgtgcg gtctctcgtt ccccgagctc gagccacggc agttctcctt
     2221 caactcaccg ttcggcgcct gtccctcatg tcacggtgtt ggcactcggc gggaggtgaa
     2281 cgccgagctg attctcggcg atctgagcat ttcgattctc gagggcgtca ttctgccgtg
     2341 gggcgagccg agtggctacc tccggaaagt ggtgctgccg acgctggcca gggccttcaa
     2401 gttcgacctt gccgctccct ggggagagct gagcgaggat gcccgacgtg cgatcctgta
     2461 cggggcgccg ggtcgcttcc gcttcgcggt ggacggtggc aaaggcc
//
LOCUS       DASRJQ010000257         4549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2143656, whole genome shotgun sequence.
ACCESSION   DASRJQ010000257 DASRJQ010000000
VERSION     DASRJQ010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2143656"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..1002)
                     /locus_tag="VFB89_05805"
     CDS             complement(79..1002)
                     /locus_tag="VFB89_05805"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26849.1"
                     /translation="MKGKFVALTYLDEANVRQLLRMEELIPAVRRGLAELSAGRVAQP
                     VRVVVPVEEHAGFFGLMPAYASSALGAKLVTFYPDNSGLPTHHAVIALFQPETGEPIA
                     LMDGRLITEMRTAAASAVATDLLARRTAGVLAILGSGVQAASHLEALRLVRPIREVRV
                     WSPRNAAAFAKRHGVHAAGAAKEAVRGADLIVTATSSTTPVLRGEWVSPGAHVNAVGA
                     CRPDWRELDDDLLGKSRIWVDSKAAAVVESGDVIAAGADRVAGEIGSVASGTLGGRSH
                     DGEITVFKSLGVAVEDLVAAELVRRRSLGSD"
     gene            1146..2117
                     /locus_tag="VFB89_05810"
     CDS             1146..2117
                     /locus_tag="VFB89_05810"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HZM26850.1"
                     /translation="MITGCLVTQLIYVVAKIGVADRLATRSSTADDLAAATGVHPRAL
                     YRVLRALASLGVFAEDDRGRFSLTEAAAALRSDAPDSLQAFAMLWGEVLWPSCGALLH
                     SVRTGQPAFEHLHGMGVFEYLPQHPQAAEIFDRAMTNLTRRLAPPVVAAYDFGSLNTV
                     VDVGGGQGILLAAVLRANPRLKGVLYDRPDVLATARRTLAGEGVLERCELVRGDFFSS
                     VPAGGDAYLLKDVLHDWDDRRAVAILTTCARAMAPSARLLVVERSLPEANLAGPAKLV
                     DITMLAVTGGRERSEREYADLFRQAGLRLTRAVPTGSEMQLFEAAAA"
     gene            2118..3596
                     /locus_tag="VFB89_05815"
     CDS             2118..3596
                     /locus_tag="VFB89_05815"
                     /inference="COORDINATES: protein motif:HMM:NF018471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HZM26851.1"
                     /translation="MRPSFVLSRWLFLRLLGVVYLVAFASLVVQLPGLIGEHGLLPVG
                     EYLERASEMYGDGAWRLLPTLLWLDASDRGLLIISAGGMLLAGLLIIDVIPLIVLPAL
                     WLLYLSLTVGGQDFLSFQWDGLLLETGLLAALWAPAGWFPSLRLEERQPSPIVQWLLW
                     LLLFKLMFLSGATKLLSGDPTWRNLTALNVYFETQPLPPWTAWFVHQLSEPVHAVATA
                     LVFVVEMVIPLVIFAPRGFRWLRLAGAVLMIGFQLTLAATGNYGFFNLLAIVLCIPLL
                     DDNLLRRIIRLKLVAPKPESPFRRTVVSVAAAGFIVLSGLSFVRELVYTYPAAGPGSM
                     PLWSEQLLTTVAPFRSINGYGLFRVMTTERPEIIVEGSDDGTRWKAYEFRWKPGDPQR
                     RPRFVAPYHPRLDWQMWFAALDPNGNRALLEALVGRLLEGSSDVLGLMGPNPFPDHPP
                     KFIRLVYFRYFFSSPETRESTGAWWSREPRGDLTQPVSLPGR"
     gene            3923..>4549
                     /locus_tag="VFB89_05820"
     CDS             3923..>4549
                     /locus_tag="VFB89_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HZM26852.1"
                     /translation="MAAVKTSKRTVRRQPKPAALPRALDDGRESLDLYLDEISRIPLL
                     TVDEEKALARKAFRGDIGAQEKLARHNVRFVVSVAKQYQNRGVPLMDLIGEGNVGLMT
                     AARKFDPDRGVKFISYAVWWIRQAIQAALARHGRPVRVPLNRTADLNRLARATTYLAE
                     RLGRIPTTEELVEATGLTPESVRSLGALHIEALRLDQPMRADDDRSRME"
BASE COUNT          719 a         1480 c         1475 g          875 t
ORIGIN      
        1 atcgaggcgc cggacccctg gcgcaatgtg atgcggcgga agcaaagcct gcgtcgcgcg
       61 gtgcaggcgc tccgcgacct aatcggatcc gagtgaacga cgccgcacca gctccgctgc
      121 caccagatcc tcgaccgcga ctcccagcga cttgaagacg gtgatctcgc cgtcgtggga
      181 gcggccgccc agcgttccgg atgctaccga tccgatctcc ccggcaaccc ggtctgctcc
      241 cgcggcgatc acatcccccg attcgaccac cgccgcggct ttggagtcga cccaaatcct
      301 ggactttccc agcaggtcgt cgtccagctc ccgccagtcc gggcggcatg ctcccaccgc
      361 attgacatga gcgccaggcg agacccactc gcctcgcagg accggcgtcg tcgagctggt
      421 cgctgtcacg atcaagtcgg ccccgcgaac cgcctccttc gcggctcctg ccgcgtgaac
      481 gccgtgacgc ttcgcaaagg ccgcagcatt gcgaggactc caaacccgca cctcgcggat
      541 cgggcgaacc agccgaagcg cctcgaggtg gctggccgcc tggactccgg atcccagaat
      601 agccaggacg cccgcagtcc gacgcgccag taggtcggtc gctacggcgg aagctgccgc
      661 cgtccgcatc tcggtgatca accgcccgtc catcagggca atcggctctc ccgtttcggg
      721 ctggaagagc gcaatgacgg catggtgggt cgggaggcca gagttatccg gatagaaggt
      781 gaccagcttg gcacccaacg cgctcgaagc atacgccggc atgaggccga agaatccggc
      841 gtgctcctcg acaggcacca ccactcgcac cggctgagcc actcggccgg ccgagagttc
      901 agccagccct ctacgcactg cgggaatgag ctcctccatg cggagcagct ggcggacgtt
      961 ggcttcatcg agataggtga gggccacgaa ctttcccttc acagggctcg gggacagtag
     1021 gatgcgatca aacggagcgc caggggctcg gctggacgag gacagcggcg ccgcgcatgt
     1081 tgggtgcgac tccgaccgat cgatctccga tggcttcagc gaaaccatcc gacgcgttgc
     1141 gtcaaatgat cacgggttgt ctggtcaccc aactgatcta tgtcgtagca aagattggcg
     1201 tggccgaccg cctcgcaacc aggtcctcga ctgccgatga tctcgccgca gccacgggcg
     1261 ttcatccgcg ggcgctctat cgggtgcttc gagccctcgc gagcttgggc gtctttgccg
     1321 aggatgaccg gggccggttt tcactcaccg aagcggcggc agcgcttcgc tcggatgctc
     1381 cggattcgct ccaagcgttt gccatgctct ggggagaggt gttgtggccg tcgtgcgggg
     1441 cgcttctcca tagcgtgcgg acgggccagc ccgcattcga gcacctgcac ggaatgggcg
     1501 tcttcgagta cctccctcaa catcctcagg cggccgagat cttcgaccgg gccatgacca
     1561 atctcacccg ccgcctcgca ccgccggtcg ttgccgcgta cgacttcggc tccctgaata
     1621 cggtcgtcga cgtggggggc ggccagggca tcctgctcgc ggcagtcctt cgggccaatc
     1681 ccaggttgaa aggcgtcctc tacgatcggc ccgacgtgct cgcgaccgcc cgccgcacgc
     1741 tggccggcga gggtgtgctc gagcgctgtg agctggtgcg cggcgatttt ttcagctccg
     1801 tcccggccgg cggcgacgcc tatctgctca aggatgttct ccatgactgg gacgaccgtc
     1861 gggcggttgc catactgacg acgtgcgccc gagctatggc gccttccgcg agactgctcg
     1921 tggtcgagcg ctcgctgccg gaggccaatc tcgcgggccc ggcaaagctg gtggatatca
     1981 cgatgctggc ggtgaccggc ggccgcgagc gcagtgaacg ggaatacgcc gatctgttcc
     2041 ggcaggcggg tctccggttg actcgtgccg tgccaacggg ctcggagatg cagctgttcg
     2101 aagccgcagc ggcgtgaatg cgccctagct tcgttctctc ccgctggctg ttccttcggt
     2161 tgctcggcgt cgtttatctg gttgcattcg cgtccctggt ggttcagttg cccgggctga
     2221 tcggcgaaca tggcctgctt ccggtcggcg agtatctgga gcgggccagc gagatgtacg
     2281 gcgacggtgc ctggcgcctc ctgccgacgt tgctgtggct cgacgcaagc gaccgtggcc
     2341 ttctcatcat ttcggccggg ggaatgctgc tggccggtct gctcatcatc gacgtaatcc
     2401 cgctgattgt cctgccggcg ctgtggttgc tctatctctc gctcactgtc ggcgggcagg
     2461 atttcctctc gttccagtgg gacggcctcc tcctcgaaac cggactgctc gccgcgctct
     2521 gggcgccggc cggctggttt ccgagcctcc gcctcgagga gagacagccc tcgcccattg
     2581 tgcagtggct cctctggctc ttgctgttca agctgatgtt cctttcgggg gctaccaagc
     2641 tgctgagtgg tgaccccacc tggcgcaatc tgaccgccct caacgtctac ttcgaaaccc
     2701 aacctcttcc tccctggaca gcgtggtttg tgcaccagct ctcagaaccg gtgcacgcgg
     2761 tcgcgacggc gctcgtgttc gtcgtagaga tggtgatacc gctggtgatc ttcgcaccgc
     2821 gtgggtttcg ctggctgcgg ctcgccggtg cggtcttgat gatcggcttt cagctcaccc
     2881 tcgcagccac cggcaactac ggattcttca acctgctggc catcgtgctc tgcattccgc
     2941 tgctggatga caacctgctg cggcgaatca ttcgcctgaa gctggtggca cccaaaccgg
     3001 aatcgccctt tcgccgcacc gtcgtcagcg tcgcggctgc aggcttcatc gtcctgtccg
     3061 ggctgagctt cgttcgtgag ctggtttaca cctatccagc ggcggggccc ggcagcatgc
     3121 cgctttggag cgagcaacta ttgacgaccg tcgcgccatt tcggtccatc aatggctacg
     3181 ggctgtttcg ggtcatgacg actgaacgtc cggagatcat cgtggaggga agcgatgacg
     3241 ggacccggtg gaaggcgtac gaattccgct ggaaacccgg cgatccacag cgccgccccc
     3301 gtttcgtagc gccttaccac ccccggctcg actggcagat gtggtttgct gcgctcgatc
     3361 cgaacgggaa ccgagcgctg ctcgaagcgt tggtaggacg actgctggag ggcagttcgg
     3421 atgtcttggg gttgatgggc ccgaatccct ttccggacca tccgcctaag ttcatacggc
     3481 tcgtatactt ccgctacttt ttcagctcgc cggaaacccg agagtccacc ggagcctggt
     3541 ggtccaggga gccaagaggg gatctgaccc agcccgtgtc gctcccgggg cgttgaagcg
     3601 tcccccgagt ggcttcttga ggcgggcgga ctggccgttc ctggccagcc ggaaggtgtt
     3661 cctggccagc cggaaggtga cccggaacca ggtctctggg tcatttgttg acccatattc
     3721 ggggggtggg ttcccttcgc gcggcatcgg tgtattttgt cccgcccccg cgacggtcgc
     3781 ggcctgcggc gcattaagac aagacttggt gttccgccgc cggacagtcg ggatctggaa
     3841 gccggggcga agacgttaag tcgttgctgt atcggttctt ggtcgagtcg gccgcaacga
     3901 cccatgatga cagggagcaa acgtggcagc agtcaagacc tccaagcgta cagttcgccg
     3961 tcagccgaag cccgctgccc tgccgcgggc gctggacgac gggcgtgaaa gcctggacct
     4021 ctatctggac gagatcagcc ggattccgct gctcacggtg gacgaggaga aggctctcgc
     4081 gcggaaggca tttcgcggag acattggcgc ccaggaaaag ctcgcgcggc acaacgtgcg
     4141 gttcgtcgtc tcggtggcca agcagtatca gaaccggggc gtgccgctca tggatctcat
     4201 cggcgagggc aacgtcggtc tgatgaccgc cgctcgcaag ttcgatcctg accggggtgt
     4261 gaagttcatc tcctatgcgg tgtggtggat tcgtcaggcg attcaggcag cgctggctcg
     4321 gcatggtcgc ccggtgcggg ttccgctcaa ccggaccgcg gatttgaacc ggttggcccg
     4381 tgcgaccacg tatctcgctg agcggctggg ccggattccg acgaccgagg agctggtcga
     4441 ggcgacaggt ctgacccccg agtcagttcg gtcactggga gcgctgcaca tcgaggcgct
     4501 ccggctcgat cagccgatgc gtgctgacga cgaccggtcg cggatggag
//
LOCUS       DASRJQ010000258         2441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2790200, whole genome shotgun sequence.
ACCESSION   DASRJQ010000258 DASRJQ010000000
VERSION     DASRJQ010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2790200"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VFB89_05825"
     CDS             complement(<1..302)
                     /locus_tag="VFB89_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26853.1"
                     /translation="MVSLSVGRAVGVLGVIMLSAASVQSQAAPAPTAPPKRIIIRAGR
                     LIDGAAENARSDQGILVEGDRIVAVGPYSEVSGRASGAERIDLSGMTVLPGLIDNHT"
     gene            388..1206
                     /locus_tag="VFB89_05830"
     CDS             388..1206
                     /locus_tag="VFB89_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26854.1"
                     /translation="MGLPRRLLHTVFAGVIGVSSGPDTLADPAFLRYAPGQLDCARFQ
                     ETSRGNVETYTAGRARRESVGLEGEWRFRAQPGRADSVQVEAWFDTLSVWRRTAEANL
                     RPDTDPVIGGRYRGVVSRDGRYRADARPFVPDDIAEVADLSRALDDLLPRLPTQPLPV
                     GATWTDEAGLEIRRLADSAGVDSVLRFRSTLRRETREGALRGDSMPLRLTQRIREDGS
                     FAWHARRGLLRRERHITIETEVPPEPRVQRAVRARVEQNVVLERIPDECEPTQR"
     gene            complement(1207..2010)
                     /locus_tag="VFB89_05835"
     CDS             complement(1207..2010)
                     /locus_tag="VFB89_05835"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM26855.1"
                     /translation="MSAAATAWGIVVRDVIRSTRQRSRLLSGLARPFIWVFLVGAGFN
                     GIARLQDGMSYQAFAYPGGVVMAALFGGMLTAISTVYDREFGMLRLMLASPSGVGAVL
                     SGRTLSAALVGSMQATVVLICAPLAVHLTLAQYAAAAAALVLVSLVSGTLGLLVASRL
                     TSVENFGGVINVVLFPLLFLSGALYPTAGMPPLLRAIARANPVSYMVDLMRHAFGQGA
                     EFSLRLDLAALIGTAIVGFGLTALLFDPEQRFVGKKPSGEGRLTAVVQP"
     gene            complement(2007..>2441)
                     /locus_tag="VFB89_05840"
     CDS             complement(2007..>2441)
                     /locus_tag="VFB89_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26856.1"
                     /translation="TVGLDPEHRDRMWSLLDTERRERGATILFSTHYLSEAEPADRVV
                     MLARGEVVADAPPFALRSGLGDHIAEVDGDGADALISALSDSGVVVVAQRTGRGWRVG
                     VRGGGEVVTERAAAVSGISRLSVRPPTLEDVYFARTQVEVES"
BASE COUNT          466 a          815 c          793 g          367 t
ORIGIN      
        1 gtgtggttgt cgatcagacc gggcaagacg gtcatcccgc tgagatcgat acgttcggca
       61 cccgatgcac ggccgctgac ctccgaatac ggaccaacgg cgacgatgcg gtcgccctca
      121 accaggatgc cctgatctga gcgggcgttt tcagcggcgc catcgatcag gcgccccgcg
      181 cggatgatga ttcgcttggg tggagcagtg ggtgcgggag cggcctggga ctgtactgac
      241 gccgcactca gcatgatgac accaagaaca ccgaccgctc tgccgaccga gagtgagacc
      301 atatgagctc cgggggaggt acgttgccgg acgtgcggtc cgaccggacg gcagcaaatt
      361 acaaagtcgc catcgaatgg ggagcgcatg ggactaccac ggcggctgct ccacactgtg
      421 ttcgccgggg tcataggtgt gagctcaggc cctgacaccc tggccgaccc cgcttttttg
      481 cgatacgcac ccggccagct cgactgtgcc cgcttccagg agaccagtcg aggcaacgtg
      541 gagacttaca ccgcgggacg tgcgcgccga gagtcggtcg ggctcgaggg cgagtggcgc
      601 tttcgagccc agcccggccg cgccgacagc gttcaagtag aggcctggtt cgacacactc
      661 tcggtctggc gccgcactgc agaggcgaat ctcagaccgg acaccgatcc tgtcattggc
      721 gggcgctatc gtggcgtggt gagtcgggat gggcggtata gggccgatgc caggccattt
      781 gtgcctgatg acatcgcgga ggtagcggat ctctcgcggg cgctggacga tctactgcct
      841 cgccttccca cccagccgct tccggtggga gcaacctgga ccgacgaggc gggtctcgag
      901 attcgccgcc tggcagacag tgccggcgtc gattcggtgc ttcgcttccg cagtacgctc
      961 cggcgcgaga ctcgagaggg ggcgcttcgc ggcgacagca tgccactcag gctgacccag
     1021 aggatccgcg aggacggcag ctttgcctgg cacgcccggc gcggactcct tcggcgcgag
     1081 cgccacatca ccatcgaaac cgaggtcccc cccgagccga gggttcagcg tgcggttcga
     1141 gccagggtgg agcagaacgt cgtgctcgag cggatcccgg acgagtgcga gcctacccag
     1201 cgataactag ggctgcacga ctgcagtgag tcgaccttcg cctgaaggct ttttccccac
     1261 gaaccgctgt tccggatcga acagcagcgc ggtgagacca aagccgacaa tggccgtgcc
     1321 gatcagagca gccagatcga ggcgaagact gaactccgct ccctggccga aagcatggcg
     1381 catcagatcc accatatagg agacgggatt cgcccgtgcg atcgcccgaa gcaggggcgg
     1441 catcccggcg gtgggataca gggcgccact taggaagagc agcggaaaca acaccacgtt
     1501 gatcactccg ccgaaattct ccaccgaggt gagccgtgag gcgaccagca gcccaagcgt
     1561 accgcttacc agcgaaacca gaaccagggc ggccgccgcc gctgcgtact gggccagtgt
     1621 caagtgaacc gcgagcggag cgcagatgag cacgaccgtg gcctgcatgc tgcccacgag
     1681 cgcggccgat aaggttctgc cggagagaac ggcaccgacg ccggacgggc tcgccagcat
     1741 gagccgcagc atgccaaatt cgcgatcgta gacggtcgaa attgccgtca gcattccacc
     1801 gaacagcgct gccatcacga cgccccccgg atatgcgaac gcctgatagc tcattccatc
     1861 ctgcagccgg gcaatgccgt tgaagccggc gccgaccagg aatacccaga tgaacgggcg
     1921 ggccagcccg gacaaaagcc ggcttcgctg gcgagtgctg cggatgacgt ccctcacgac
     1981 gatgccccag gctgtcgcgg cggcgctcac gactctacct cgacttgggt tcgcgcgaag
     2041 tagacatcct ccagcgtggg cggccgcacg gacaggcggc tgattccgga aaccgccgcc
     2101 gcccgctcgg tcaccacctc tcctccccct cgcacaccca cccgccagcc ccggccggtc
     2161 cgttgcgcga cgaccacgac ccctgagtcg ctcagggcgg agatcagcgc atccgcaccg
     2221 tcgccgtcga cctcggcgat gtggtctccc agccccgatc gcagagcgaa gggcggtgca
     2281 tcggccacga cttcgccgcg agcaagcatc accacgcgat cggccggctc ggcttcggac
     2341 agatagtgag tcgagaagag aatggtcgcg ccccgctcgc gtcgctcggt atcgagcagg
     2401 ctccacatgc gatcgcgatg ctccggatcc agcccgacgg t
//
LOCUS       DASRJQ010000259         5610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_910182, whole genome shotgun sequence.
ACCESSION   DASRJQ010000259 DASRJQ010000000
VERSION     DASRJQ010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5610
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910182"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VFB89_05845"
     CDS             complement(<1..346)
                     /locus_tag="VFB89_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26857.1"
                     /translation="MSRQIRGELVHVVGEIAPGARGTRHSRLPTELAFDTDLTGHVGN
                     LIGEGGERLDHAVDRIGQSRDLALGFHAELALEIAVGDGGDDLGDAAHLTRQVARHRV
                     HVVGEILPDAGDA"
     gene            458..3235
                     /locus_tag="VFB89_05850"
     CDS             458..3235
                     /locus_tag="VFB89_05850"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZM26858.1"
                     /translation="MIRNLKDTTQKTTEQDWLKTNLARFTRMLQGQRDLVAVGQQILS
                     ELAPLVNAQHGLIYITGTEDGITKLKLLASYAATDEVPQSFALGEGLVGQCASERQRI
                     LLNQIPPESILIHSGLVTAHPRTVIVLPVLFEGQVKAVIELASLADFTTSHLAFLEQL
                     TGSIGIVLNTIEATMRTESLLEQSQQLAGELQTQQKELQQTNEELAQKAKLLAEQNAE
                     VERKNQEIEQARRALEEKATELALTSKYKSEFLANMSHELRTPLNSILILGQQLGENS
                     EGNLSPRQVEFARTIHGAGTDLLNLITDILDLSKIESGTVTVVAEEVVVTGLLDAVAR
                     PFRHEAETRHLSLDVQVDNDLGRTIVTDSKRLQQVLKNLLSNAFKFTEQGGVRLSVTP
                     AATGWSSDHPVLKRAANVVAFEVSDTGIGIPQEKQRIIFEAFQQADAGTSRKYGGTGL
                     GLAISRELASLLGGEIQLRSMPGLGSTFTLYLPQAYVGPAAQGSISTTAETEARTARP
                     PAAFPAVRISERPVEAIPDDRHDLKPDDAILLIVEDDPAYARVLLDQAHAQGFRVLVA
                     SRGAEALDLAREYRPTAVSLDVFLPDMLGWTVLNQLKQDPAMRHIPVQIVTLDEDRQH
                     GLARGAFSFLPKPTTREGLEAAFTRIKDFATPRSKRLLVVEDNEAEQLSIAELLGHHD
                     VEIETAATGREALAALDRTPYDCAVLDLRLPDMSGFEVLEHIRDNQALSDLPVVVFTG
                     KELSPEEDARLHTLARSVVVKGVESPERLLDETALFLHRVIANLPPEKQAMLDRLHRS
                     DDDLIGKKVLVVDDDMRNIFALSSVLERRGMEVLTAGTGHEAIATLEATPDAAIVLMD
                     IMMPEMDGYQTMHAIRQKPRFRRLPIIALTAKAMKGDREKCLDAGASDYLAKPVNTEQ
                     LLSALRMWLHR"
     gene            3242..4864
                     /locus_tag="VFB89_05855"
     CDS             3242..4864
                     /locus_tag="VFB89_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZM26859.1"
                     /translation="MANNGSDKVNILLVDDQPGKLLTYEAMLSELGENLIKANSGREA
                     LEHLLKTDITVVLMDVSMPELDGFELAEIIRQHPRYQKTAIIFVSAVHLTDLDRLKGY
                     ASGAVDYVSVPVVPELLRAKVSVFAELYRKTRESERLNRELEQRVAERTAELEASNAR
                     QTELAEQLRHADRRKDEFLALLAHELRNPLAPVRNAVTIMRLKNSDDPELTWCRDVIE
                     RQANQLTRLVDDLLDVSRITRGKITLRIETLDLVTVIDAAVETSRPLIDAHKHDLVVT
                     LPKNRVRIEGDLTRLTQVVANLLNNAAKYQNERGRIELDAVCERGEAVISVTDHGVGI
                     APEMLSQVFELFSQGERAPDRAQGGLGIGLSLVKNLVEMHGGSVGAVSAGLGKGSKFM
                     VRLPTVSSEPAALTSLPPDSAMVPAPHPLRVLVVDDSPDAAESLAKLLRLAGHEVLVA
                     HDGPRALELAAADRPTVMLLDIGLPGMDGYEVCRRMRQQGFADARIIAVTGYGQDRDR
                     QRSREAGFDSHAIKPVDPSELMKLVTGPVSHP"
     gene            4903..>5610
                     /locus_tag="VFB89_05860"
     CDS             4903..>5610
                     /locus_tag="VFB89_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26860.1"
                     /translation="MTQATVPLTRQRFLATRRRDAWWVKPLVILIILGSFVAYSTWAA
                     FQNAHYTFGPYLSPFYSPELFGSSPHAIFGPKPGWWPGVLPFSPALLILPFPAFFRFT
                     CYYYRGSYYKSFWADPPACSVGEPRNTYLGERSFPLILQNVHRYFMYAAVVFLFILSY
                     DAWKAMWWTDAGTGTVRFGIGLGTLLLIVNIVCLTGYTLGCHSLRHILGGFRNQFSKK
                     PLLKTPYKCVTCLNGVHH"
BASE COUNT         1036 a         1908 c         1790 g          876 t
ORIGIN      
        1 gggcgtcgcc ggcgtctgga aggatctcac cgacaacgtg aactcgatgg cgagcaacct
       61 gacgggtcag gtgcgcggca tcgccaaggt cgtcaccgcc gtcgccaacg gcgatctcaa
      121 gcgcaagctc agcgtggaag ccaagggcga gatcgcggct ctggccgata cgatcaacgg
      181 catgatcgag acgctcgcca ccttcgccga tcaggttacc aacgtggccc gtgaggtcgg
      241 tatcgaaggc aagctcggtg ggcaagcgcg agtgccgggt gccgcgggca cctggcgcga
      301 tctcaccgac aacgtgaacc agctcgccgc gaatctgacg actcaagtgc gtgcgatcgg
      361 cgacgtggcg acggccgtga cgaagggcga cctcactcgc tcgatcacgg tcgaagcgtc
      421 gggcgaggtg gcggctctca aagacaacat caacgagatg atccgcaacc tcaaggacac
      481 cacccagaaa acgaccgagc aagactggct gaagaccaac ctcgcgcgct tcacgcgcat
      541 gctccagggc cagcgcgacc tcgtcgcggt gggacagcag atcctctccg agctcgcacc
      601 actcgtcaac gcgcagcacg ggctcatcta catcacgggc accgaggacg ggataacgaa
      661 actgaaactg ctcgcgagct acgccgccac ggacgaagtc ccgcagagct tcgcgctcgg
      721 cgagggtctc gtcgggcagt gcgcgtccga gcgccagcgc atcctcctga accagatccc
      781 gcccgagtcg atcctgatcc actcggggct cgtgaccgcg cacccgcgca cggtgatcgt
      841 gctcccggtg ctcttcgagg gccaggtgaa agccgtgatc gagctcgcct cgctggccga
      901 tttcacgacg tcgcacctcg cgttcctcga gcagctcacg ggcagcatcg gcatcgtcct
      961 gaacacgatc gaggcgacga tgcggacgga gagcctgctc gagcagtccc agcagctcgc
     1021 cggcgagctc cagacgcagc agaaggagct ccagcagacg aacgaggagc tggcgcagaa
     1081 ggccaagctg ctcgccgagc agaacgccga ggtcgagcgc aagaaccagg agatcgagca
     1141 ggcgcggcgg gcgctcgagg aaaaggccac cgagctcgcg ctcacctcga agtacaagtc
     1201 cgagttcctg gccaacatgt cccacgagct gcggactccg ctgaacagca ttctgatcct
     1261 gggtcagcag ctcggtgaga actccgaggg gaacctgtcg cctcggcagg tcgagttcgc
     1321 ccgcacgatt cacggggccg gaaccgacct gctcaacctc atcaccgaca ttctcgacct
     1381 gtccaagatc gaatccggga ccgtgacggt cgtcgcggag gaagtggtgg tcaccggcct
     1441 gctcgatgcg gtggcccgcc ccttccggca cgaagccgaa acccgccacc tctccctcga
     1501 cgtgcaggtg gacaacgatc tcgggcgcac catcgtcacc gactcgaagc gcctccagca
     1561 ggtgctcaag aacctgctgt ccaacgcctt caagtttacc gagcagggcg gcgtccgcct
     1621 gtcggttaca cccgccgcga ccggctggag ctccgaccat ccggtgctca agcgcgcggc
     1681 gaacgtggtg gccttcgagg tatcggatac cggcatcggc atcccgcagg agaagcagcg
     1741 catcatcttc gaagccttcc agcaagccga cgccggcacc agccgcaagt acggcgggac
     1801 cggactcggc ctcgccatca gtcgcgagct ggccagcctg ctcggcggcg aaatccagct
     1861 ccggagcatg ccggggctcg gcagcacctt caccctctac ctgccccaag cctacgtcgg
     1921 gccggcggcg cagggcagca tcagcacgac cgccgagacg gaagcccgga ccgcgcggcc
     1981 cccggcggct ttcccggccg tccgcatcag cgagcggccg gtcgaggcga ttcccgacga
     2041 ccggcacgac ctcaagcccg acgatgccat tctcctcatc gtcgaagacg acccggccta
     2101 cgcgcgggtg ctgctcgacc aggcccacgc gcagggcttc cgggtcctgg tggcgagccg
     2161 cggcgccgaa gcgctggacc tggcccgcga gtaccgtccc acggcggtgt cgctggacgt
     2221 cttcctgccc gacatgctgg gatggaccgt gctgaaccag ctcaagcagg acccggccat
     2281 gcggcacatt ccggtgcaga tcgtgaccct cgacgaggac cgccaacacg gcctggcgcg
     2341 gggcgcgttc agcttcctcc cgaaacccac cacccgtgag ggactggaag cggccttcac
     2401 gcggatcaag gattttgcga cgcctcgatc gaagcgactg ctcgtcgtgg aagacaacga
     2461 agccgagcag ctgagcatcg ccgagctgct gggacatcac gatgtcgaga tcgaaacggc
     2521 cgcgaccggc agggaagcgc tcgcggcgct ggaccggacc ccctatgact gcgccgtgct
     2581 ggacctgcgc ctcccggaca tgtcgggctt cgaggtgctg gagcatatcc gcgacaacca
     2641 ggccctctcc gatctgccgg tggtcgtctt taccggcaag gaactgtcgc ccgaggagga
     2701 cgcccggttg cataccctgg cgcggagcgt ggtggtcaag ggggtggaat cgcccgagcg
     2761 gctgctcgac gagacggcgc tcttcctgca ccgcgtcatc gccaacctgc cgccggaaaa
     2821 gcaggccatg ctcgaccggc tgcaccggtc cgacgacgac ctgatcggca agaaggtgct
     2881 ggtggtagac gacgacatgc gcaacatctt cgcgctctcc agcgtactgg agcggcgcgg
     2941 gatggaggtg ctcaccgcgg gcacagggca cgaagccatc gcgacactcg aggccacgcc
     3001 cgacgccgcg atcgtcctca tggatatcat gatgccggaa atggacgggt accagacgat
     3061 gcacgccatc cggcagaagc cccgtttccg tcgccttccc atcattgccc tcacggccaa
     3121 ggcgatgaaa ggcgaccggg aaaagtgcct tgatgccggc gcatcggact acctggcgaa
     3181 accggtcaat acggagcagc ttctgtcggc gctccgcatg tggcttcacc ggtagccggc
     3241 catggcgaac aacggcagcg acaaggtcaa catcctcctg gtcgacgacc agcccggcaa
     3301 gctcctcacc tacgaggcga tgctgagcga gctgggcgag aacctgatca aggccaattc
     3361 cggccgcgag gcactggagc atctgctcaa gaccgacatc accgtggtcc tgatggatgt
     3421 gagcatgccg gagctggacg ggttcgagct ggccgagatc atccggcaac atccccggta
     3481 tcagaaaacc gccatcatct tcgtgtcggc cgtccatttg accgacctgg accggctcaa
     3541 ggggtacgca tcgggcgcgg tggactacgt gtcggtgccg gtggttcccg agctgctccg
     3601 ggccaaggtg agtgtcttcg cggagctgta ccgcaagact cgagaatcgg agcggctcaa
     3661 ccgcgagctg gaacagcggg tagcggagcg aaccgccgag ctggaggctt ccaacgcgcg
     3721 gcagaccgag ctggcggagc agctgcgtca cgccgaccgc cggaaggacg agttcctggc
     3781 cctgctggcc catgagctgc gcaatccgct ggccccggtg cgcaacgccg tgaccatcat
     3841 gcgcttgaaa aactcggacg accccgagct gacctggtgc cgagacgtca tcgagcggca
     3901 ggccaaccag ctcacccgcc tggtcgacga tctgctcgac gtgtcgcgca tcacccgcgg
     3961 caaaatcacg ctccggatcg agaccctgga cctcgtcacc gtcatcgacg ccgcggtgga
     4021 aaccagccgt cccctgatcg atgcccacaa gcacgacctg gtagtgacgc tgccgaagaa
     4081 ccgcgtccgg atcgaggggg acctgacccg gctcacccag gtggtcgcca acctgctcaa
     4141 caacgccgcc aagtatcaga atgaacgggg ccggatcgag ctcgacgccg tctgcgagcg
     4201 aggcgaggcg gtgatcagcg tcaccgacca tggggtcggg atcgcgccgg aaatgctgtc
     4261 ccaggtcttc gagctcttct cccagggcga gcgggcgccg gaccgcgctc agggaggcct
     4321 gggcatcgga ctgtcactgg tcaagaacct ggtcgaaatg cacggcggct cggtgggcgc
     4381 ggtgagcgcg ggactcggca aaggcagcaa gttcatggtg cggctgccca ccgtctcgtc
     4441 ggagccggcc gcgctgacca gcctcccgcc cgactcggcc atggtcccgg ccccccatcc
     4501 gctccgggtc ctggtagtgg atgacagccc ggacgccgcc gagagcctgg ccaagctgct
     4561 tcggctggcg ggtcacgagg tgctggtggc tcatgacggg ccgcgggcgc tcgagctcgc
     4621 cgcggccgac cggccgacgg tgatgctcct cgacatcggc ttgcccggaa tggatggata
     4681 cgaggtgtgc cggcggatgc gtcagcaggg tttcgctgat gcccggatca ttgcggtcac
     4741 cggctacgga caggaccgcg accgccagcg ctcgcgggag gccgggttcg attctcacgc
     4801 catcaagccg gtggatccct ccgaattaat gaagctggtg acagggccgg tgtcccaccc
     4861 ttagcttacc gggtcatttc tcgagcctct cggagctgac ccatgaccca ggcgacggtg
     4921 cccctcactc ggcagcggtt tctcgccaca cgccgtcgcg atgcctggtg ggtcaagccg
     4981 ctcgtcattc tgatcattct gggcagtttc gtggcctatt ccacctgggc ggcgttccag
     5041 aacgcgcact acaccttcgg gccgtatctc tcacccttct acagccccga gctgttcggc
     5101 agctccccgc acgccatctt cggccccaag ccggggtggt ggccgggggt tctgccgttc
     5161 agccctgccc tgctgatcct gccgttcccg gcatttttcc ggttcacctg ttactactat
     5221 cgcggctcgt attacaagtc gttctgggcc gatccgcctg cgtgcagcgt gggcgagccg
     5281 cgcaacacgt atctgggcga gcgttccttc ccgctgatcc tgcagaacgt acaccgctac
     5341 ttcatgtacg ccgcggtggt attcctgttc atcctgtcgt acgatgcctg gaaggcgatg
     5401 tggtggaccg acgccggcac cggaacggtg cgattcggaa tcgggctggg caccctgctg
     5461 ctgatcgtca acatcgtgtg cctgaccggc tacacgctgg ggtgccactc gctgcgacac
     5521 atcttgggcg gattccggaa tcagttctcg aaaaagccgc tgctcaagac cccctacaag
     5581 tgcgtcacct gtctcaacgg agtacaccac
//
LOCUS       DASRJQ010000260         7498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1218560, whole genome shotgun sequence.
ACCESSION   DASRJQ010000260 DASRJQ010000000
VERSION     DASRJQ010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7498
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218560"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="VFB89_05865"
     CDS             <1..953
                     /locus_tag="VFB89_05865"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM26861.1"
                     /translation="PVLLAAGLGIAFRSRPSERTAIAIPRSIPHAEHVAEALRRDDRL
                     AVRVLDDSVATQALRIGDVALMLATGSGNKIEYRYDDRRPESRTARLIVDDALQRAAG
                     RSDPIPVSDRHVSEPGSRYIDFVVPGLLGMNLMGSGIWGIGFAIVDARRKRLLKRLIA
                     TPMSRSQYLASFVLSRLTWLVLEVGLLLGFAVLVFGVPVRGSLLELTVICLLSALSFA
                     SIGLLLASRARTVEGVSGLMNLVMLPMWILSGVFFSSSRFPDVIQPLIRALPLTAVIN
                     ALRATMLQGAPFQGVALELAIITVWLVVSFPLALRMFRWK"
     gene            complement(959..1252)
                     /locus_tag="VFB89_05870"
     CDS             complement(959..1252)
                     /locus_tag="VFB89_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26862.1"
                     /translation="MIIRWLAVAGALSVTALLTNPASAQAQAADASPGTRALLGSPTS
                     SGSFGNLEVRPDDGTATPAERGLLGVASGVRVELTAEPALHRRSAGEAALLGR"
     gene            complement(1351..1713)
                     /locus_tag="VFB89_05875"
     CDS             complement(1351..1713)
                     /locus_tag="VFB89_05875"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZM26863.1"
                     /translation="MPRPIHFEIPADSPERAIEFYQKVFGWEFQKWDGPMPYWLVRTG
                     TGTGIDGGLLPRAYPGQGTVNTVDVSSCDEFVRKAESAGGKVAVPKMAIPGVGWLAYC
                     TDPEGNTFGVMQSDSRAR"
     gene            complement(1745..2110)
                     /locus_tag="VFB89_05880"
     CDS             complement(1745..2110)
                     /locus_tag="VFB89_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26864.1"
                     /translation="MNKPVLGLLLGGALGALDGLTALISAPEVRPEIVGIVIGSTGKG
                     LLAGLLIGFVARKVNNLTAGILIGTVIAALLALPIALHADPVTGKRYIWEIMIPGSIV
                     GLIVGYATQRFGRPVAQRS"
     gene            complement(2199..3167)
                     /locus_tag="VFB89_05885"
     CDS             complement(2199..3167)
                     /locus_tag="VFB89_05885"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZM26865.1"
                     /translation="MTLPDDTLAHLRRVLQTPELPGDRYEICESIGHGGMGTLYRAHD
                     RALARDVALKVLRSDVAGPEAAVRLEREAKILAQLEHPGIVPVHDVGVLADGRVFYVM
                     KLIRGERLHDFARGTSRTDVLRVMLRVCETVGFAHAHGVIHRDLKPSNIMVGSFGEVL
                     VLDWGIARVRGVAEQDGPSIAGLAVATSKEDTAPGVVLGTPGFMAPEQAQGLSAQVDG
                     RTDVYALGAILRAVAGADIHAGPVPRPLQAIWSRASSPDPAGRYASAAELAFDLTRFL
                     DALPVEAYREGLGERVGRVFRKYQTAIILILTYLAVRLLFLVLRGL"
     gene            complement(3164..3970)
                     /locus_tag="VFB89_05890"
     CDS             complement(3164..3970)
                     /locus_tag="VFB89_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26866.1"
                     /translation="MSSRVRWRLGLRACNFPVAMFPHPPTPHGGFPTTRRSVVEAIRD
                     ADPDIRRDALDSLVAVYWRPVYAHLRRKWRFQPADSEDLTQEFFAQAVTGEFLRGYDP
                     TQARFRTYLRACLDRFVANAQRNGQRLKRGGGATLLSLDFDLIERELAAGDGLIEDSS
                     DSWFDREWIRGLFAAAVADLRSACQATPREIRFTVFERYDLRPVRDGDRPAYRTIAEE
                     LGLPVTQVTNHLAWARREFRRLVLERLRSLTFTDAEFREEAEELLGEKRS"
     gene            4030..4677
                     /locus_tag="VFB89_05895"
     CDS             4030..4677
                     /locus_tag="VFB89_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26867.1"
                     /translation="MKKLAIGCAIFIMVCVVGGAIGSYYVYHKVRSTIGGFAELGKVP
                     EIERSVRNQSPYVPPSSGEISTAQLQKFLAVQQKVRARMGVRAAEMERTYHTLLSKKE
                     ATALDMPELVAAYRDLAGVYVDGKRAQVDALNAAGFSLDEYRWVRTQSYAALGVPMMD
                     FDVTRMIEEAKAGRTPTPPNRVITVGPTGPPANQKLVKPYEKVITDYAPFGFFGL"
     gene            complement(4835..>7498)
                     /locus_tag="VFB89_05900"
     CDS             complement(4835..>7498)
                     /locus_tag="VFB89_05900"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM26868.1"
                     /translation="TLPVADRLRLFLQVCDAVSYAHGRGVIHRDIKPLNIIVTPAGVP
                     KLLDFGIAKVLHDAGDEATSTVTGLRLLTPEYASPEQVEGHHATAASDVYSLGVVLYE
                     LLTGRSPYRLSSRAPQDVAAAVCTTDPERPSVAVSRAPDVAGDSFRRRRRESAANHAA
                     VSSGVSARQLSRLLRGDLDTIILAALRKEPARRYPTVAAFADDLRRHLEGRPVLARSE
                     GVLYRARKFVQRNRAAVAAAGLAMVTAVMLAAVVRPPAPRAEAEEPSLLSNGVLAPRD
                     RILIADFTATNGDTALAIAITEAFRVDLAQSPNVQVMTPRQVRSALERLERTAELAVN
                     DSLAREIALREGVKAFVVGRVSNIAGRWAVNVELMGAESGDVLAAVRETAPDSTDLIG
                     AVDRASETLRHRLGESLRELRALPPLRKEMTASLPALRKYTEAARLDFASKRTLALQL
                     LHEATALDSNFAIAHLAIANTYDAMAEPGRALQSLRRALAHLDRLPYRDRQYLVGTRA
                     YAREDWDTAIEAYTRYLERYPGYVPALNNLALAYRDARRLAPAETLWARAVSLDSTIA
                     QLYYGVHSVQLLAGRFKDSHGTLDLIGRRFPDNPILLGTEVQDAAAQHDWETAERRAE
                     ANIAAQQGDTLALVDPFEQMAGIVMTQGRLAEAERYWRTQLMLSARSESWGRRLFGAR
                     QLGYLRLRYRNSPSAAIAIVDSALARQPLDSTLPGDRPYYELARFFAEAGDVPRAHRL
                     VADGRVNDSLLDLPRRTERAWTAGVIALAEGRAAEAEVALRDAAHTHVCAICPLPDLA
                     RAYEARGNAAAAIGAYERYLTTPWFFRYEVDASQLGFVLKRLGELYEARGDHQKAAEV
                     RTRLLALWHRADAELQPLLADVRTRVTPPAH"
BASE COUNT         1238 a         2465 c         2524 g         1271 t
ORIGIN      
        1 ttccggtact gctcgccgcc ggtcttggca ttgccttccg cagccgtccg agcgagcgca
       61 ctgccatcgc gatcccgcgg tcgattcctc acgcggagca tgtcgcggaa gccttgcgtc
      121 gggacgatcg cctggccgtc cgcgtgctgg atgattcagt cgcgacgcag gcgctgcgta
      181 tcggcgacgt tgcgctcatg ctggccacgg gaagtggcaa caagatcgag tatcgctacg
      241 atgatcggcg accggaaagc cggactgcgc gcctgatcgt agacgatgcg ctccagcgag
      301 ccgcggggcg cagcgatccg attccggtgt ccgatcgcca cgtgagcgaa cccggttccc
      361 gctatatcga cttcgttgta ccggggcttc tgggaatgaa tctgatggga agtgggatct
      421 gggggatcgg gttcgcgatt gtggacgcgc gccgcaagcg gctgctcaag cggctcattg
      481 caaccccaat gtcccgatcc cagtatctcg cctcgtttgt gctgtctcgg ctcacctggc
      541 tcgtcctcga ggtcggcctc ctgctcggct tcgccgtgtt ggtgtttggg gtgccggtcc
      601 gagggtcgtt gctcgaactg acggttatct gcctcctgtc cgcgctctcg tttgcgtcga
      661 tcggactcct gctggcgtcc cgcgctcgca cggtggaagg tgtttccggg ctgatgaacc
      721 tggtgatgct cccgatgtgg atcctttcgg gcgtcttctt ctcgtcgtca cgcttccccg
      781 acgtgattca gccgctcatt cgggcactgc ccctcacggc tgtcatcaat gcgctccggg
      841 ccacgatgct ccagggcgca ccatttcagg gtgttgccct ggagctggcc attatcaccg
      901 tttggctcgt cgtcagcttt cccctcgcgc tccggatgtt tcgctggaaa tagccttgct
      961 aacgccccaa cagcgctgct tcgccagctg atctccgatg cagcgccggc tcggccgtga
     1021 gctcgacgcg aacgccggat gcaactccga gcagaccgcg ctcagctggt gtagccgtac
     1081 cgtcatccgg tcgcacttcc agattcccga aactacctga gctggtggga ctgcccagca
     1141 gtgcccgtgt ccctggcgag gcgtcggcgg cctgtgcctg agcgcttgcg gggttggtga
     1201 ggagcgccgt cacgctgagg gcgcctgcga ccgcgagcca tctgatgatc attgcacctc
     1261 ccgtggaaac cgcccgccgc cgagcgcggc gggatcacgg gcttcaacga cgcgtccacg
     1321 gttcgggttc ccggctccgc cacattgccc tcaccgggcc cggctatcgc tctgcatgac
     1381 cccaaacgtg ttcccctcgg gatcggtgca atacgccagc cagcccacgc ccggaatcgc
     1441 catcttgggc acggccacct tgccgcctgc gctttccgcc ttgcggacga attcatcgca
     1501 ggaggagacg tccacggtgt ttacggtccc ctgaccggga tacgcgcggg gaagaagtcc
     1561 gccgtcgatg ccggtgccgg tcccggtgcg cacgagccag tacggcatcg gcccgtccca
     1621 cttctggaac tcccagccga agaccttttg gtagaactct atggcccgct cggggctatc
     1681 ggccggaatc tcgaaatgga tgggccgggg catggttcct cctgtgacgc agggttgagt
     1741 tgactcagga tcgttgagcg acgggtcgcc cgaatcgctg ggtagcgtat cccacgatga
     1801 gaccgacaat cgagccgggg atcatgatct cccagatgta gcgcttgcct gtgacgggat
     1861 cggcgtgcag cgcaatcggg agtgcgagca acgcggcgat cacggtgccg atcagaatgc
     1921 cggcggtcag attgttcacc ttgcgtgcga cgaagccgat cagaagtccc gccagaagcc
     1981 ccttgcccgt cgagccaatg acaatgccca cgatctccgg tcgaacctcg ggtgcggaga
     2041 tgagggccgt cagtccgtcg agagcaccaa gggcgccgcc gagcagcaga ccgagcacgg
     2101 gcttgttcac aaacgcctcc ggattgagga cgcgggatcg cgaagctctc tgcagttcta
     2161 ttacccgtcg ggatggaatc ctttaaacag gtccggtact agagtccccg cagcacgaga
     2221 aacagcaggc gaaccgccag ataggtaagg atgaggatga tggcggtctg gtacttccgg
     2281 aacacccggc caacccgttc gcccaagccc tcccgatacg cctcgaccgg gagcgcgtcg
     2341 agaaacctgg tgagatcgaa ggccagctcg gcggcggagg cgtaacgtcc cgccggatcg
     2401 ggcgacgaag cccggctcca gatggcctgc agggggcgcg gcaccgggcc cgcatgaatg
     2461 tcggcgccgg cgacggcgcg caggatcgcg ccgagcgcat agacatcggt tcgaccgtcg
     2521 acttgtgcgg aaagcccctg agcctgttcc ggagccatga acccgggagt tccgaggaca
     2581 acgccgggcg cggtatcctc cttcgaagtc gcgaccgcca ggccggcaat tgatggccca
     2641 tcctgctcgg ccactccgcg cactcgcgca atcccccagt ccaggaccag cacctcgccg
     2701 aaactgccga ccataatgtt ggacggcttg agatcgcgat ggatgacgcc gtgggcgtgg
     2761 gcgaacccga ctgtttcgca gacgcgcagc atgacccgca ggacatccgt gcgactggtt
     2821 cccctggcga aatcgtgcag ccgctctcct cgtatcaact tcatcacgta gaacacgcgc
     2881 ccgtccgcca gcacgccgac atcgtgcact ggcacgatgc ccggatgttc gagctgcgcg
     2941 agaatcttcg cctcgcgttc gagccgtacc gccgcctccg ggccggcgac gtcggagcgt
     3001 agaaccttca gggcaacgtc acgcgcaagc gcacggtcat gcgcccggta tagcgttccc
     3061 atcccgccgt gcccgatgga ctcgcagatc tcgtagcggt cgccgggcag ctccggggtt
     3121 tggagcactc gtcgcaggtg agcgagcgta tcgtcgggca gcgtcacgat cgcttctcgc
     3181 cgagcagctc ctcggcttcc tctctgaact cggcgtcggt gaacgtcagc gagcgcagcc
     3241 gctcgaggac cagcctgcgg aactctcgcc gtgcccaagc cagatggttg gtcacctgag
     3301 tcaccggcag cccaagctcc tcggcgatgg tgcgataggc cggtcgatcg ccgtcacgca
     3361 caggcctgag gtcgtagcgc tcgaagacgg tgaaacggat ttcgcgcgga gtcgcttgac
     3421 acgcagatcg cagatcggca acggcggcgg cgaagagacc tcgaatccac tcccggtcga
     3481 accagctatc ggagctgtcc tcgatgagcc cgtccccggc ggccaactcg cgctcgatca
     3541 gatcgaagtc gagcgacagc agcgtcgcgc cgccaccgcg ctttagccgt tgtccatttc
     3601 gctgagcgtt ggcgacgaac cggtccaggc aggcgcggag gtaagtgcga aaacgggcct
     3661 gagtggggtc gtacccgcgg aggaattctc cagtcaccgc ctgggcgaag aactcctggg
     3721 tgaggtcctc ggaatcagcg ggctggaacc gccatttccg gcggagatgg gcatacaccg
     3781 gtcgccaata caccgcgacc agcgagtcaa gagcgtcgcg ccggatatcc gggtctgcgt
     3841 cacggatcgc ctcgaccacc gagcgccggg tggtcgggaa acctccatgc ggagttggcg
     3901 ggtggggaaa catggcgacg ggaaagttgc aggcgcgcag gccgagccgc caccggaccc
     3961 ggctcgacaa ggcgatccgg tcgaagcatg ttcttgcgac cctcagcaat ccgtgccggc
     4021 ggaggcttca tgaaaaagct cgcgatcggg tgtgccatct tcatcatggt gtgtgtggtg
     4081 ggcggggcga tagggtcgta ttacgtgtac cacaaggtgc gctcgaccat cggcggcttc
     4141 gccgagctgg gcaaggtccc cgaaatcgag cggtcggtcc gcaatcagtc gccctatgtg
     4201 cctccctcga gcggcgaaat ctccacagcc caattacaga aattcctcgc ggtccagcag
     4261 aaggtccggg ccaggatggg cgtccgagcc gccgagatgg agcgcaccta ccataccctt
     4321 ctgtcgaaga aggaagcgac ggcgctggac atgcccgagc tggtggccgc ctatcgggac
     4381 cttgccggcg tatatgtgga cggcaagcga gcgcaggtgg atgcgctcaa cgcagccggc
     4441 ttctcactcg acgagtatcg gtgggtccga acccagagtt acgcggcact cggcgtgccc
     4501 atgatggact tcgatgtgac cagaatgatc gaagaggcca aggccggccg cacgcccacg
     4561 ccaccgaacc gcgtcattac cgtcggtcct accgggccgc cggccaatca gaagctggtg
     4621 aagccttacg agaaggtgat cacggattac gcgccgttcg gatttttcgg gctctagttc
     4681 gcttcagctt gggccgagtg cacacgatat cccacgttgc gaacggtaag gatgtgtcgc
     4741 agccgggtgg gatcgtcctc aaagcgcggc agcagcgaag gcgcgcgcga aatcctgacc
     4801 ggtgtggcag ctggcgtctt ggcgccggcc tgccctagtg tgcaggtggg gttacccgcg
     4861 tgcggacgtc tgccaggagc ggctgcagct cggcgtcggc acgatgccag agggcgagca
     4921 ggcgcgtccg cacctccgcg gccttctggt ggtcgccacg agcctcgtac aattccccga
     4981 gtcgcttcag aacgaaaccg agctggctcg cgtcaacctc gtaccgaaag aaccagggcg
     5041 tggtgagata gcgctcgtac gcgccgatcg cggcggctgc gttcccccga gcctcgtacg
     5101 cgcgcgcgag gtcggggagc gggcagatgg cgcagacgtg cgtgtgtgcc gcatcacgca
     5161 gtgcgacttc ggcctcggcg gccctccctt ccgcgagcgc gatcaccccg gcggtccagg
     5221 cacgttcggt tcggcgtggg aggtcgagga ggctgtcgtt gaccctcccg tccgccacga
     5281 ggcggtgcgc gcgcgggacg tctcccgcct ccgcgaagaa gcgggcgagc tcgtagtagg
     5341 gacggtcacc cgggagcgtg ctgtcgagcg gctggcgcgc gagcgcggag tccacgatgg
     5401 cgatggcagc actgggcgag ttccggtacc ggagtctgag gtacccgagc tgccgagcgc
     5461 cgaacaggcg gcgtccccac gactcgctcc gagccgagag catgagttgg gtgcgccagt
     5521 agcgctccgc ttcggccaga cgcccctggg tcatgacgat ccccgccatt tgctcgaacg
     5581 ggtcaaccag cgccagcgtg tcaccttgct gcgccgcgat gttcgcctcc gcccggcgtt
     5641 ccgctgtctc ccagtcgtgt tgcgcggccg catcctgcac ctcggtcccg agcaggatgg
     5701 ggttgtccgg gaagcgccga ccgatcagat cgagtgtccc gtgcgagtcc ttgaaacgac
     5761 cggcgaggag ctgcacgcta tgcacgccgt agtagagctg cgcgatcgtg gaatcgaggg
     5821 acactgcgcg tgcccagagt gtttctgctg gcgccaggcg gcgagcgtca cggtaggcga
     5881 gcgcgagatt gttgagcgcg gggacatacc ccggatagcg ctcgagatac cgggtgtatg
     5941 cctcgatcgc cgtgtcccag tcttcccggg cgtatgcccg ggttccgacc agatactgcc
     6001 ggtcgcgata gggcagacgg tcgaggtggg ccagtgcgcg cctgagtgac tggagagccc
     6061 gccccggttc tgccattgcg tcgtaggtat tcgcaatcgc gagatgcgcg atggcgaagt
     6121 tgctgtcgag cgcagtggcc tcgtggagca gctggagtgc cagggtccgc ttgctcgcga
     6181 aatcgagccg tgccgcctcg gtgtacttgc ggagcgccgg cagcgaggcg gtcatttcct
     6241 tcctgagcgg cggcagcgct cgaagctcgc ggagggactc gcccaggcgg tggcgcagcg
     6301 tctcgctcgc gcgatccacc gcgccgatga gatcggtcga gtcgggtgcc gtctcgcgga
     6361 ccgcggccag cacatcccct gattccgccc ccatcagctc cacattgacg gcccagcgcc
     6421 cggcgatgtt ggagaccctc cccaccacga atgccttcac cccttcgcgc agcgcgatct
     6481 cgcgtgccag cgagtcattc acggccagct ccgcggtgcg ctccaggcgc tccagggcgc
     6541 tccgcacctg tcgcggcgtc atgacctgca cattgggcga ctgcgccagg tccacccgaa
     6601 acgcctcggt gatcgcgatc gcgagggcgg tgtcgccgtt ggtggcggta aagtcggcaa
     6661 tgaggatccg gtctcggggc gccaacaccc cattcgagag gagagacggc tcctcggcct
     6721 cggcccgcgg ggcgggcggg cggacgacgg ccgcgagcat taccgccgtc accatggcga
     6781 gcccggcggc ggcaacagcg gcgcggttcc gctgcacgaa cttccgggcg cgatagagca
     6841 cgccctcgct gcgggctagg accggccgcc cctcgaggtg ccggcgcaag tcgtcggcga
     6901 aggcggcgac ggtggggtaa cggcgggcgg gctccttgcg aagcgccgcg aggatgatcg
     6961 tgtcgaggtc gccccggagc agccggctca attggcgcgc gctcaccccg ctggatacgg
     7021 ccgcatggtt cgccgcggac tcgcgacggc ggcgccggaa cgaatcaccc gccacgtcgg
     7081 gtgcccggga gaccgccacg ctcggccgct cgggatcggt ggtgcaaacg gcggcggcca
     7141 cgtcctgcgg ggcccggctg gagagccggt acggcgagcg gccggtcagc agctcataga
     7201 gcaccacacc gagggagtag acgtcgctcg cggcggtggc gtggtgcccc tccacctgct
     7261 ccgggctggc gtactccggg gtgagcagcc gcagcccggt gacggtcgag gtggcctcgt
     7321 cgcccgcatc gtgaagcacc ttcgcgattc cgaagtcgag cagcttcgga acccccgccg
     7381 gtgtcacgat gatgttgagc ggcttgatgt cccggtggat gacaccccgc ccgtgggcgt
     7441 aggaaaccgc atcgcacacc tgcaggaaga gccgcagccg gtcggcgacc gggagcgt
//
LOCUS       DASRJQ010000261         2507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2305802, whole genome shotgun sequence.
ACCESSION   DASRJQ010000261 DASRJQ010000000
VERSION     DASRJQ010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2305802"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="VFB89_05905"
     CDS             <1..833
                     /locus_tag="VFB89_05905"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM26869.1"
                     /translation="ARNGVARGWIELKHTFINAQDLMGYLFPAVLLLTVMFFMRDATV
                     PGTDFSLGARTLPSALGMGIVFGGMVNLAQQLMVEREDGTLLRAKATPNGMLGYLVGK
                     ITVASGMLLISLLLQLIPGLFLLEGLAITTVSAWLTLTWVLVVGLVATLPLGAVVGSL
                     FENPRNFGIVMLPLMGLIATSGIFYPLTNYPTWLQWVGQAFPVYWLGLGMRSALLPDE
                     MATVEFGQSWRHLETLGVLGAWAVAGLILAPIVLRRMARRESGSSVAARREKALQRAT
                     "
     gene            924..1196
                     /locus_tag="VFB89_05910"
     CDS             924..1196
                     /locus_tag="VFB89_05910"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZM26870.1"
                     /translation="MSERETIYNRIAMLRAERGISRRQLADALGVHYQTVGYLERGEF
                     RPSLYLALRLAQYFEVPVEVIFATEPFPRLGETQQEHWTRPGTVAR"
     gene            1193..1840
                     /locus_tag="VFB89_05915"
     CDS             1193..1840
                     /locus_tag="VFB89_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26871.1"
                     /translation="MTLSDPLPKGWFGTDLGPFRASSGTYELYPADSTASRLEFDGTF
                     GWLPTRREAGIEYMVPVSAETALAVSKAPGLPPSFVSFMRRPDLTGAIPTCTSCHWNL
                     VESAIPSPLDDGARLLGFMNDQQDVLLWYLYLEPDGGHRVVCGSIPYHDVTVESGRAA
                     ADLVEVAPNFEAFIYRFWVENLAWYELNEFGRADTELTAAVREYLARYPTAPGSG"
     gene            complement(1868..>2507)
                     /locus_tag="VFB89_05920"
     CDS             complement(1868..>2507)
                     /locus_tag="VFB89_05920"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZM26872.1"
                     /translation="EYGVRALGVTLSRRQAEWAQKAIAEAGLGELAEVRHLDYRDVEE
                     TQFDAISSIGLTEHIGVKRLPDYFSFLYRRLRPGGRLLNHCITVPDNRTGVLERGFIS
                     RYIFPDGELTGAGRIISAMHDNGFEVRHEENLREHYALTSAAWAANLDAHWNESVAEV
                     GVAKARTWRLYLAGVRLGFERNAIQLHQVLGVKLHEDGRSDMSLRPDWEPVT"
BASE COUNT          393 a          809 c          845 g          460 t
ORIGIN      
        1 ccgcgcgcaa tggggtcgcc cgcggctgga tcgagctgaa gcacaccttc atcaacgctc
       61 aggaccttat gggctacctt ttcccggccg tgctgctgtt gaccgtcatg ttcttcatgc
      121 gcgacgcgac cgtgccgggg acggacttct cgctaggagc taggacgctt ccgagcgcgc
      181 tcggcatggg catcgtgttc ggcgggatgg tcaaccttgc ccagcagctg atggtggagc
      241 gggaggacgg cacgctgttg cgcgcgaagg ccacaccgaa cgggatgctc ggctatctcg
      301 tcggaaagat cacggtcgct tcggggatgc tactgatcag cctcctcctg cagctgatcc
      361 cgggtctgtt tctcctcgag ggactggcca tcaccacagt cagtgcgtgg ctcactctta
      421 cctgggtgct cgtcgtggga ctggtggcga cattgccctt gggcgcggtc gtgggctccc
      481 tgtttgagaa tccacgtaac ttcggcattg tgatgcttcc gctcatgggt ctgatcgcga
      541 cgtcgggcat cttctaccca ctcacgaact acccgacctg gcttcagtgg gtcggtcagg
      601 catttcccgt gtactggctc gggctcggca tgcgttcggc actgttgccg gacgagatgg
      661 cgacagtcga gtttggacag tcgtggcggc accttgagac tttgggcgtg ctgggcgcgt
      721 gggccgtcgc gggcctgatc ctggccccga tcgtcttgcg ccggatggct cgccgcgagt
      781 cgggctccag tgtcgcggcg cggcgcgaga aggccctgca gcgggcgaca tgagaggtcg
      841 cccatgaacg agcgcagcga gagaatcatc aggctcagta cgaccgagcc tcatcgagcc
      901 ggggagcgaa gcgaggcggc gagatgagcg aacgcgagac catttacaac cggatcgcca
      961 tgttgcgggc cgagcggggg atctcgcgcc ggcaactcgc cgacgcgctc ggcgtgcact
     1021 accagacggt gggttacctc gagcgcggcg aattccggcc gagcctgtat ctcgccctgc
     1081 ggctggcaca gtactttgaa gtgccggtgg aggtgatctt cgccaccgag cccttccccc
     1141 gcctgggcga aacacaacag gaacactgga cccggcccgg tacggtggcg cgatgacatt
     1201 gagcgacccg ctcccgaaag gttggttcgg aaccgacctg ggacccttcc gggccagcag
     1261 cggcacctac gagctgtatc ccgccgacag cacggcgtcg cggctcgagt tcgacggtac
     1321 gttcggttgg ttgcccacgc gccgtgaggc ggggatcgaa tacatggtgc cggtgtccgc
     1381 ggagaccgca ctggcagtct ccaaggcgcc cggcctgcca ccgtcctttg tgtccttcat
     1441 gcgtcggccg gatctcaccg gcgcgattcc aacctgcacg tcgtgccact ggaacctggt
     1501 cgagtcggcg attccgagcc ccttggatga cggcgcgcgg ctgctgggct tcatgaacga
     1561 ccagcaggat gtgctgctgt ggtacctcta ccttgagccc gacggcggtc accgcgtggt
     1621 gtgcggcagc attccgtatc acgacgtcac cgtcgagtcg gggcgtgcgg ccgcggacct
     1681 ggttgaggtg gctcccaact tcgaagcgtt catctaccgg ttctgggtag agaacctggc
     1741 ctggtacgag ctgaacgagt ttggccgggc tgacacggaa ctcaccgcgg ccgtgcggga
     1801 gtacctcgcc cgttatccca cggcgcccgg ctccggctga tgtagtccac tgtggtcgga
     1861 agcgaggtca ggtcaccggt tcccagtcgg gacgcagcga catatccgag cgcccgtcct
     1921 cgtgcagctt gacaccgagc acctggtgca actggatggc gttgcgctcg aacccgagcc
     1981 ggacaccggc gagatagagc cgccaggtgc gggccttggc cacgcccacc tcggccacgg
     2041 actcattcca gtgcgcatcg aggttcgcgg cccacgcggc gctagtgagc gcgtagtgct
     2101 cacgcaggtt ctcctcgtgc cgcacctcga agccgttgtc gtgcatggcg gagatgatgc
     2161 ggcccgcgcc ggtgagctcc ccgtcgggga agatgtagcg gctgatgaag ccccgctcca
     2221 gcacgccggt ccggttgtcc ggcacggtga tgcagtggtt gagcaggcga ccccctggtc
     2281 gcagccgccg gtacaggaag gagaagtaat ccggcaaccg cttgacgccg atgtgctcgg
     2341 tcaaaccgat cgagctgatt gcgtcgaact gggtctcctc gacgtcgcgg tagtcgaggt
     2401 ggcgcacctc ggccagctcg cccaggcccg cctcggcgat cgccttctgg gcccactcgg
     2461 cctgccggcg ggacagggtc acgcccagcg cccgtacccc gtactcg
//
LOCUS       DASRJQ010000262         3681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2577239, whole genome shotgun sequence.
ACCESSION   DASRJQ010000262 DASRJQ010000000
VERSION     DASRJQ010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2577239"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1483
                     /gene="gpmI"
                     /locus_tag="VFB89_05925"
     CDS             <1..1483
                     /gene="gpmI"
                     /locus_tag="VFB89_05925"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HZM26873.1"
                     /translation="REGNAIELADTPVWHRLWSSAQRTLLEASGLRVGLPEGQMGNSE
                     VGHLNLGAGRVVPQDLVRISQSIASGEFYRLEPLVELGAFLRQSGGTLHLVGLLGPGG
                     VHALDRHLLACIELAVRLKVPAIAIHGFLDGRDAPPTLGAEVVRTLLQDMRRIAGNRV
                     EVATLCGRYYGMDRDRRWERTKVAYDAIVHSKGHAITHPVLAVQAAYQRGETDEFLKP
                     LVHYRDNEPVARLRTGDGVLFFNYRSDRMRQIVAAMCLDNFHGFDVRDRPVLRGVTMT
                     QYDQTFPLPQAFPPFTLARILAQVLADAGMTQFRTAETEKYPHVTYFFNGGVEPPYPS
                     EERCLIPSQRVATYDLAPEMSAEGITDALCRTLEAKAHDFLLCNYANADMVGHTGVLP
                     AVIRAVETVDRCLDRVLASAERSGASVLVTADHGNAETMIDPSTGGVHTAHTTNPVPL
                     VAVRGECSALRSGGALCDVAPTVLGLLGLEQPADMTGRDLRQC"
     gene            1477..2157
                     /locus_tag="VFB89_05930"
     CDS             1477..2157
                     /locus_tag="VFB89_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26874.1"
                     /translation="MLITKPAFAAITALALVCPVRAIAQVGYPPKSSPYHDLHKGSSI
                     TFTGGYFMGNGGRAGVGPRDGYIVGGRFDLHASRPLQFGLGVEYGSLQRNIVDPTQPP
                     GSRVTGPVDQSVTFVEGLVTFNITGGKTWHGLAPFIEMAVGAAFGSSTPADTSGYNFG
                     TKGFFAPGGGLRFFLGDRFHIRAAARGNFWKLSYPPSYRTGAVPVTLDASEWNLSPWF
                     EFGLGFIL"
     gene            2172..2588
                     /locus_tag="VFB89_05935"
     CDS             2172..2588
                     /locus_tag="VFB89_05935"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HZM26875.1"
                     /translation="MPTTLTRTVSFFARHRLYRPEWSPEQNREAFGALSEEPPHGHQY
                     QCAVTVEGGPNAPADLLVDLAELDRILREEVVEPLNDKYLNRDIPAFARALPTCEAMA
                     QHLFRRISARLPKGTTLRRVRVAENPSLYADCSGDP"
     gene            2593..3183
                     /locus_tag="VFB89_05940"
     CDS             2593..3183
                     /locus_tag="VFB89_05940"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HZM26876.1"
                     /translation="MIAGAILAGGDATRFGGRPKGLEMVGGKRILDRLVDAFEGALHR
                     LPLLVANAPDAPEWRSDLRVVPDVRPGCGSLGGLYTAVIEAPAPVVCVAWDMPFVTAD
                     LLARLAAGVDGCDVFLPESEGPRRGEPLCAAYGPACAPAMASRLDAGDLRATGFHSAV
                     RVCTLRLEQVRRLGDPARLFFNVNTADDLAKADALS"
     gene            3183..>3681
                     /gene="mobB"
                     /locus_tag="VFB89_05945"
     CDS             3183..>3681
                     /gene="mobB"
                     /locus_tag="VFB89_05945"
                     /inference="COORDINATES: protein motif:HMM:NF015181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HZM26877.1"
                     /translation="MRILTVIGRKNAGKTTLTVALAGELIRRGRRIMTIKHGHHPADV
                     DRTGSDTYRHFQEGRAERTLIAAPTLRVLFERTPDDYDPVALARRYMDGADLVLCEGF
                     KQASLPKIEVWRRAIGGKPLYDPTAANASEWIAIMTDDRDMGVEATCPVFSFNDTNWL
                     QTLASM"
BASE COUNT          626 a         1193 c         1195 g          667 t
ORIGIN      
        1 acgcgagggc aacgccatcg aactcgctga cacacccgtc tggcatcgct tatggtcctc
       61 cgcgcaacgg accctgctcg aggccagcgg gctccgcgtt ggacttcccg aagggcaaat
      121 gggcaacagt gaagtcgggc atctcaatct cggggccggc cgcgttgtcc cgcaggatct
      181 ggtgcggatc tcacagagca tcgcatcggg cgagttctat cgactcgagc ccctggtcga
      241 gttgggtgcc ttcctgcgtc agagtggagg aaccctccat ctcgtgggcc tgctcgggcc
      301 gggcggcgtc catgcactgg atcgccatct gctcgcttgc atcgagctgg ccgtcaggct
      361 caaggtgccg gccatcgcca ttcacggctt cctcgacggc cgcgacgcac cgcccacgct
      421 gggcgccgag gtggtccgca cgctcctgca ggatatgcgg cgcatagctg ggaaccgggt
      481 cgaagtggcc accctgtgcg gccgctatta cgggatggat cgcgatcgcc gctgggagcg
      541 caccaaggtc gcctacgacg cgatcgttca cagcaaggga cacgcgatca cgcatcccgt
      601 gctcgcggtc caagccgcct accagcgagg cgaaaccgac gagtttctca agcccctggt
      661 tcattatcgg gataacgagc cggtcgcacg acttcgaacc ggcgatggcg tgctcttctt
      721 caattatcgc agtgatcgga tgcgtcagat cgttgcagcc atgtgtctcg acaactttca
      781 cggcttcgac gtgcgcgacc gtccggtgct gcgtggcgtt acgatgacgc agtacgatca
      841 gacgtttccg ttaccgcagg cctttccgcc gtttacgctg gcccgaattc tggcgcaggt
      901 gctcgcagat gcgggaatga cccagtttcg aaccgcggag accgagaagt atccccacgt
      961 gacttacttc ttcaacggcg gtgtcgagcc gccctatccc agcgaggagc gctgtctgat
     1021 cccttcgcag cgcgtcgcta cgtacgatct ggcgcccgag atgagcgccg agggaattac
     1081 cgatgcgctt tgccggactc tcgaggcaaa ggcgcacgac ttcctgctct gcaactacgc
     1141 caatgcggac atggtgggac acactggagt gctgcctgcg gtcatccggg cggtcgagac
     1201 ggtggaccgc tgcctcgacc gggtccttgc gagtgcagaa cggtccggtg cgagcgtcct
     1261 tgtgacagcc gaccacggta acgcagagac catgatcgat ccctccacgg ggggcgtaca
     1321 taccgctcac acgacgaacc cggttccctt ggtcgctgtt cgcggtgaat gctccgcgct
     1381 gcgctcgggc ggcgcgctgt gcgatgtcgc tccgaccgtc ctgggccttc tcggtctgga
     1441 gcagccggcc gacatgaccg gacgcgatct ccgccagtgc tgattacgaa gccagcgttc
     1501 gccgcgatca ccgcgctcgc cctcgtgtgc ccggtgaggg caatagcgca ggtgggatac
     1561 ccaccgaaga gcagccccta tcacgacctt cacaaaggca gcagcatcac gttcaccggc
     1621 ggatacttca tggggaacgg gggtcgcgcc ggtgtcggtc cgcgtgacgg gtacattgta
     1681 ggcgggcgat tcgacttgca cgcgtctcgg cccctgcaat tcgggctggg cgtcgaatac
     1741 ggaagcctgc agcgaaacat cgtcgacccc acacagccgc caggatcgcg ggtgaccggg
     1801 ccggtcgacc agagcgtcac cttcgtcgag gggctggtga cgttcaacat caccggcggg
     1861 aaaacctggc acggcctggc gccgttcatc gagatggccg tcggcgcggc gtttggcagc
     1921 agcacgccgg ccgataccag cggttataac ttcgggacca agggattctt tgcgcctgga
     1981 ggaggccttc gattctttct gggcgaccgg ttccacatcc gcgcagcggc ccggggaaac
     2041 ttctggaagc tgtcctatcc gcccagctat cggaccgggg ccgtcccggt cacgctcgac
     2101 gcgagcgagt ggaatctcag cccttggttt gagtttggtc taggattcat cctctagcag
     2161 gccacggggg catgccgacc acgctcactc gcactgtgag cttcttcgcg cgtcaccggc
     2221 tctaccggcc ggagtggagt ccggagcaga accgcgaggc gttcggcgcc ttgtccgagg
     2281 agccaccgca cggccaccag taccagtgcg ccgttacggt ggagggtggc ccaaatgcac
     2341 ccgcggatct cctggtcgat ctcgcggagc tcgaccggat cctccgcgag gaagtggtgg
     2401 agccgctgaa cgacaagtat ctcaatcggg atattcccgc gttcgcccga gcgctgccca
     2461 cctgtgaggc catggcccag caccttttcc gtcgaatctc cgcgcgcctt ccgaagggga
     2521 ccacgctccg tcgcgtgcgc gtcgcggaaa accccagcct gtacgccgat tgtagcggcg
     2581 acccgtgacg ccgtgattgc cggagcaatc ctggccggag gcgacgcaac ccggttcgga
     2641 ggccgtccca aaggcctcga gatggtcggc ggaaagcgga ttctcgatcg cctggttgac
     2701 gctttcgagg gcgccctgca ccgtttgccg ctgctggtcg ccaatgctcc cgacgcgccc
     2761 gagtggcgct ccgacctgcg agtcgtgccc gatgtgcgtc ccggctgtgg ttcattagga
     2821 ggactctata ctgcggtaat cgaagccccg gcgccggtgg tgtgcgtggc gtgggacatg
     2881 ccgttcgtga cagccgattt gctcgcgagg ctcgctgccg gtgttgacgg atgcgatgtg
     2941 ttcctgcccg agagtgaggg tccccgcagg ggcgagccgc tctgcgctgc atacggcccg
     3001 gcatgcgccc ccgcgatggc gagcaggctc gatgccggcg atctgcgagc gaccggcttt
     3061 cacagcgccg tgcgggtctg tactctgaga ctcgagcaag tgcgccgcct gggcgacccg
     3121 gctcggctct tcttcaacgt caacaccgcg gacgatctgg cgaaagcgga cgccctcagc
     3181 tgatgcgcat ccttactgtg atcggacgca agaacgcggg caagaccacg cttacagtgg
     3241 ccttggctgg agagttgatt cgccggggcc gacggatcat gacgatcaag cacgggcatc
     3301 atccggccga cgtcgatcgc accggaagcg atacctaccg ccatttccag gaaggacggg
     3361 cggagcgtac cctgatcgcg gcgccgacgc tccgggtctt gttcgagcga acccccgatg
     3421 actacgatcc ggtcgctttg gcccgacgct acatggacgg cgccgacctg gtgttgtgcg
     3481 aggggttcaa gcaggcatcg ctccccaaga ttgaagtgtg gcgacgggcg atcggtggaa
     3541 agccgctcta cgatccaacg gccgccaatg cgtccgagtg gatcgcgatc atgacggacg
     3601 accgcgacat gggcgtcgag gccacttgcc cggtgttttc gttcaacgac accaactggc
     3661 tgcagacgct ggcgagcatg g
//
LOCUS       DASRJQ010000263         3669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1070015, whole genome shotgun sequence.
ACCESSION   DASRJQ010000263 DASRJQ010000000
VERSION     DASRJQ010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070015"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="VFB89_05950"
     CDS             complement(<1..1092)
                     /locus_tag="VFB89_05950"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZM26878.1"
                     /translation="MKKTTLPVLTRRYRWLALGGGLLAWSPTAPPFDPAGTWRAVLDL
                     AGGSLRFSVDISGRAGAWSAKLCNGDRCEPISRVRVFGDSLSLEIADYDATIDALIKG
                     DSLAGAYRNVGNRGPRVIPFRAARGSWNKAAGPPKLVGRWDATFFQAWRSSPRVFEIR
                     NGPAGLEGTIISNSGDYGHFWGSASADSFALGHFDGSFVYLLTGRLDGDTLRGVFHAG
                     LRSETPWVAVRSTGKPHLKAPTEVTRADTTEPFRFAFPDLQGHLVSNTDARFRGKVVL
                     VDVFGSWCPTCHDAAPALVRLWQKYHSQGLEIVGLAFEVTGDTAVDGRQVRRFRDKFG
                     IQFPLLLAGINDTETAAEALPQLQGFTAYP"
     gene            complement(1110..1985)
                     /locus_tag="VFB89_05955"
     CDS             complement(1110..1985)
                     /locus_tag="VFB89_05955"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HZM26879.1"
                     /translation="MSSKTVRQLALPGCLLLLQGPTCGALAAQAAVVAQERTEYERWL
                     GSANVSPYAAIAQARIGTGIRLGPADSDVPLEGVPLQRVFEEGGRVVLESAAGERLLP
                     KERPVGLSGFTLLASGPAGRATLSVFGKPALKRSPTYFPYDSSVVFSVSLERADQSGS
                     GFVLAVDGTEVEAPVAGSISFNFAGRLTRLVVRRIQGGDEESDLEIYFRDATNGKGTY
                     PAGRFVTLLPSPSGRYLLDFNRARNPFCAYNSVYACPAPWRGNTVAAPVLAGERYSGG
                     GLDAPAVADTGVKTR"
     gene            complement(1982..2785)
                     /locus_tag="VFB89_05960"
     CDS             complement(1982..2785)
                     /locus_tag="VFB89_05960"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HZM26880.1"
                     /translation="MSTPDDASIRDLAAAYALGALSPEETRAFEAYLAGSPEARQDVA
                     ELRETAALLAVAGPERQPGPELRQRVLDRVRASKFATLPATPARPPRSRIPLMVWAGL
                     AALLVIAVGLANRVRVLTSTLVVRDSTIAAQQQALSEQGSQLAAREQILASIFEPGVQ
                     LVQLTKQGDPDPRIQLFWDRLRNTAVLHASKLKPTPSGRTYQLWFIRDGKPVPSVTFD
                     VTAEGSASVSGVTVPTGGEISAAAVTEEPTGGSQQPTTPILLVGTAPKS"
     gene            complement(2782..3387)
                     /locus_tag="VFB89_05965"
     CDS             complement(2782..3387)
                     /locus_tag="VFB89_05965"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZM26881.1"
                     /translation="MPNPGSATSLPADRDLVARAAIGDETAIAQLYDRYATVLYTVAY
                     RIVGERADADEVVVEAFAQAWRDAGRFEPGRGSVAGWLTMIARSRALDLVRARSRRER
                     ITGNAAADQPDEPPAMGAWPGNPADSLDRTERRRQVLAALGELSPPQRTAIELAFFEG
                     LSQSEIAERLNEPLGTVKTRVRLGMQKLRDRLRPVFFDQGS"
BASE COUNT          655 a         1201 c         1216 g          597 t
ORIGIN      
        1 cgggtatgct gtgaatccct ggagctgagg caatgcctcg gcggccgtct ccgtgtcgtt
       61 gatcccggcc aacagcaggg gaaactgaat tccgaacttg tcgcggaagc ggcggacttg
      121 acgaccgtcg acggccgtgt cgccggttac ctcgaaggcc aggccgacga tttcaagccc
      181 ctgggagtga tacttctgcc agagtcgcac cagagccgga gctgcatcgt gacaggttgg
      241 gcaccaggat ccgaacacat cgaccaacac cacctttcct cgaaagcgtg catccgtgtt
      301 ggagaccaga tgaccctgga gatcgggaaa ggcgaagcgg aacggctcgg tcgtatcggc
      361 ccgggtgacc tcggtaggag ccttgaggtg tggcttgccg gtgctccgca ccgccaccca
      421 cggcgtctcc gatcggagtc ctgcgtgaaa cacgccgcgc agagtgtctc cgtccagtct
      481 gccggtcagc agataaacga atgagccatc gaagtggccg agagcaaagc tgtccgcgga
      541 agccgacccc cagaaatgcc cgtaatcacc gctgttcgag atgatggtcc cttccaggcc
      601 ggcaggcccg ttcctgatct cgaacaccct cgggctgctg cgccacgcct ggaagaacgt
      661 cgcgtcccag cggcccacca gcttgggtgg gccggctgcc ttgttccagc tgcctcgggc
      721 ggcccggaac gggatgactc tcggtccccg gttgccgacg ttccgataag cgccggccag
      781 cgagtcccct ttgatgagcg cgtcgatggt cgcgtcgtag tcggcgatct cgagcgaaag
      841 gctgtcgccg aacacgcgga ctcgggaaat gggctcgcat cgatcgccgt tgcaaagctt
      901 cgccgaccag gctccggccc tgccgctgat gtcgaccgag aaccgaagcg atccaccggc
      961 cagatcgagt accgctcgcc atgtgcccgc agggtcgaag gggggcgcgg tgggcgacca
     1021 cgccaggagt ccaccgccca acgccagcca acggtagcga cgagtgagca caggcagggt
     1081 cgtttttttc atgggaacac gggcggagct cagcgcgtct taacgccggt atcggccaca
     1141 gccggcgcat cgagtccgcc gccgctgtat cgctcgccgg ccaggaccgg cgcggctacc
     1201 gtattgcccc gccacggagc gggacaggcg tagaccgagt tgtaggcgca gaatggattg
     1261 cgcgctcgat tgaagtcgag cagatatcgg ccgctcgggc tgggaagcag ggtgacgaaa
     1321 cgacctgcgg ggtatgtccc ttttccgttg gttgcatctc ggaaatagat ctcgagatcg
     1381 ctttcctcgt cccctccctg gattcgacgg accacgaggc gagtcagtct tccggcaaag
     1441 ttgaacgaga tgctgcccgc gacgggcgcc tccacctccg tgccgtccac cgcaagcacg
     1501 aaaccggagc cggattggtc tgcccgctcc agcgatacgc tgaatacgac gctggaatcg
     1561 taggggaagt aggtcggcga ccgcttgagc gcgggcttac caaacaccga aagtgtcgcg
     1621 cgaccggccg gcccactcgc gagcaaggta aagccgctca acccgaccgg gcgttccttg
     1681 gggagtaacc tttcgccggc tgcgctctcc aggacaaccc ttcctccttc ctcgaaaaca
     1741 cgctgcaacg gaacaccctc gaggggcacg tccgaatcgg cgggcccgag ccgaatgccg
     1801 gtgccgatcc gagcctgcgc gatcgcagca tagggagaaa cattcgccga gccgagccaa
     1861 cgctcgtact cggtccgttc ctgcgcgacg acggctgctt gggcagcaag cgcgccgcag
     1921 gtgggaccct gaaggagcag taagcacccc ggcagggcca gctgtcggac cgtcttacta
     1981 ctcacgattt cggagcagtc cccacgagga gaatgggagt ggtcggctgt tgcgaaccgc
     2041 cagtcggctc ctcggtaact gcagccgcgc tgatctcgcc gccggtgggc acggttactc
     2101 ctgagacgga tgcgctgccc tccgccgtaa catcgaacgt taccgagggt accggtttgc
     2161 cgtcccgaat gaaccatagc tgataggtac gaccggacgg cgtgggcttc agcttcgagg
     2221 cgtgcagaac ggcagtattg cgcaagcggt cccagaacag ctggatccgc ggatcggggt
     2281 cgccttgctt ggtgagctga accagctgga cccccggctc gaaaatcgag gccaggatct
     2341 gctcccgagc agctagctga ctaccctgtt ccgatagcgc ttgttgctgg gccgcgattg
     2401 tgctgtcgcg caccacgagc gtcgaggtga gcacgcgaac ccggttggcg agtcccactg
     2461 cgatcacgag aagggcagcg agccccgccc acaccatgag gggaatcctg gaacgaggtg
     2521 gtcgtgccgg ggtcgcgggc aaggtcgcga acttgctcgc ccgaacccga tccagcactc
     2581 gctggcgcag ctccggaccc ggctgccttt caggcccggc gacagcgagc aacgcggcgg
     2641 tttcccggag ctcggcgacg tcctgccgcg cctccggcga gcccgccagg tacgcctcga
     2701 aggcccgcgt ctcttcggga gacagggcgc ccagagcgta agccgcggcc agatcgcgga
     2761 tcgaggcgtc gtcaggtgtg ctcatgatcc ctgatcgaag aaaacggggc ggagccggtc
     2821 tcgcagcttc tgcatcccca gccggaccct ggtctttacg gtgccgagcg gctcgttgag
     2881 tcgttcagct atttccgatt gcgacagccc ctcgaagaag gcgagctcga tggcggtgcg
     2941 ctggggcggc gacagctctc ccaaagcggc gagcacctgg cgtcggcgct cggtccggtc
     3001 caggctgtcg gcagggttgc cgggccaggc gcccattgcc ggcggctcgt ccggctggtc
     3061 tgcggcggcg ttgccggtga tgcgctcgcg gcgggaccgc gcgcgaacca ggtcaagcgc
     3121 ccggctccgg gcgatcatcg tcaaccagcc ggcaactgag ccacgccccg gctcgaaccg
     3181 gccggcgtca cgccatgcct gcgcgaaggc ctcgacaacc acctcatccg cgtcggcccg
     3241 ctcgcccacg atccggtagg ccacggtgta gagcaccgtt gcatatcgat cataaagctg
     3301 tgcgatcgcc gtttcgtcac cgatggcggc gcgcgccacc aggtcgcgat cggccggcag
     3361 agaagtcgcc gagccggggt taggcattgg ccatgagctg agggtatccc tcggcggcgc
     3421 cgcgaggttg tggctgtccg cgatccacag tctatatccc ggatcgaggg ccccacaagc
     3481 tcggacggca ttcatggaag tgccttggcc tgatctcccg ccgtgaacag caggctggtc
     3541 gtgcgcccgc cgtaggtcgc gcgaacgatg ttgacctggt ccgggaagta ttcgcagagc
     3601 agcaggtcac ggacgcggat gccggcgagt ccccggggca gctcggcgcg gagccgaatc
     3661 tggaagacg
//
LOCUS       DASRJQ010000264         4298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1217608, whole genome shotgun sequence.
ACCESSION   DASRJQ010000264 DASRJQ010000000
VERSION     DASRJQ010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217608"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..2536
                     /locus_tag="VFB89_05970"
     CDS             221..2536
                     /locus_tag="VFB89_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26882.1"
                     /translation="MSPITKEKPVRPRDFTTGDEIIPLMDDIIAAESVGGAEALPLPF
                     PFPFPLRLAVSGLYEYRLLLPFPPLQPQPPIVPLPIPERPGRPGLPVGAQRLDGIPTT
                     DTRLILRETLRLDVDGRYPQMAASGTIYQFLTERTHWVAQLTKTPEGDYTGPIWYLNG
                     DTTNFPYDTVTIQAISSIFANQKAAIATFTGPNGASRTRHYRHISRYFHPCEFEYDVV
                     EGTQSVTSINTGDHPNHPPGLPAEQLTIETVYRRSGFNVSQSPGGGTIPIDGAGVDQA
                     WSDSEMHDAMQVYWLRFADKAQWALWTLFASLHEQGTSLGGIMFDDIGPNQRQGTAIF
                     EDSFINQPPPNDSNPAAWIARMKFWTAVHEIGHTFNLAHSWQKSLGVPWIALADEPEA
                     RSFMNYPYAVQGGESAFFSSFEYRFSDQELLFLRHAPERFVQQGNALWFDNHGFEQTR
                     RAQLPRLSLELRVNRSPATFEFMEPVVIEFKLKNISGEQQFIDQHLLATFDRLTVVVK
                     KEGRPARQFRPFAQYCWRSGKIVLQPGESAYESLFVGSGSNGWDVAEPGRYVIQAALH
                     LVEEDIVSTPLAIRIAPPKGYDEEYLAQDLFTEDVGRVLAFDGTKVLDSANNVLKEVS
                     EKLPDSKAAIHAKVALAMPLSRPYRALKVDAKGSPDEQFVFHGDKAKLPEAVADFRSA
                     VGRRQDKSAESLGHIDYADYAIRMSQAASRAGAREEAREIGEAMLTTLNRRAVPERIL
                     TQLRERLNAPGEEGKSNGRWRKSPTRPARPK"
     gene            complement(2634..3338)
                     /locus_tag="VFB89_05975"
     CDS             complement(2634..3338)
                     /locus_tag="VFB89_05975"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZM26883.1"
                     /translation="MTYKEDPPRDDPLTRTDEMAVADGRSRRRLRPGTRRDEILEAAQ
                     AVFSELGFDRTTLNDVAERAGVTKGAVYHYFDSKDELFIALLRERLMPHIEAGEELLA
                     NTTASREQILRELLTQSWTHYQQPGQIELAILAMNELPKIPEVGRILFDEVVQRGRRM
                     MKEALARGVELGEIPSCDIDAAAAVVPYMIMGVALGHRLFRGVDPLTLSAERLGGAAV
                     AVLLRGVGAGDEDDCR"
     gene            3507..4109
                     /locus_tag="VFB89_05980"
     CDS             3507..4109
                     /locus_tag="VFB89_05980"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HZM26884.1"
                     /translation="MVTRSTAVGLLAILLPAVVSAQGPSFETKTGPSVIVFANGGGNS
                     PLTNLNQTGTADLKTGWALGGGAGVQVNQYFAVRGVFDFARSEGSANSTVFAGQRLNH
                     YFYGGDVQLRYPTSGGFAPYLLAGAGAVTIDNKDDLTSNTTTRFAGKGGVGVEYSFPR
                     NGLGLFAQGASYVYKFDRDGFDKTQFDVLWTAGLSYRFPF"
BASE COUNT          783 a         1428 c         1324 g          763 t
ORIGIN      
        1 cctcccggac accacgtcct cgaagtagca ctccctgctc gacatcggtc cgagaagatc
       61 ccgttcgacc tcgaccgaga gctccacctc gctctgagcc tcgaacgaga cggcacgctt
      121 agccaccgct cgtcggaatc cacgttcggg tacgtctgac cggttcgacg ccgccgcggt
      181 ggtcggccgg ggccgtggcg gcgcgatcag aggaggcccc atgtccccaa tcacgaaaga
      241 aaagcctgta cgcccgcggg acttcacaac aggcgacgag atcatcccgc tgatggatga
      301 catcatcgcc gccgagtccg ttggcggtgc cgaggcgcta cccctgccgt ttccgttccc
      361 ctttccgctc cgtctggcgg tgagcggact ttacgagtac cggctgctac tcccttttcc
      421 ccccctccag ccgcagccgc caatagtccc gctgcccatc ccggagcgcc ccggccggcc
      481 cggcctacct gtgggagccc agcgtctcga cggtatcccg accaccgata ctcggctcat
      541 tttgcgcgag accctgcggc tggatgtgga cgggcgctat ccgcagatgg ccgcgagcgg
      601 gaccatctac cagttcctga ccgagcggac ccactgggtc gctcaactta ccaagacgcc
      661 cgagggagac tacaccgggc cgatctggta cctgaacggc gacaccacga acttcccgta
      721 cgacaccgtc accattcagg ccatctccag catcttcgcg aaccagaagg ctgcaatagc
      781 gaccttcacc ggaccgaacg gcgcaagccg gacccgccac taccggcaca tctcgcggta
      841 cttccatccc tgcgaattcg agtacgacgt ggtggagggc actcaatcgg tcaccagcat
      901 caacaccggc gaccatccga atcacccgcc tggtctgccc gcagagcagc tgaccatcga
      961 gacggtgtac cgccgatcgg gcttcaatgt gagccagtca cccggtggcg gcaccattcc
     1021 gatcgacgga gccggggttg atcaggcgtg gtccgacagc gagatgcacg acgcgatgca
     1081 ggtctactgg ctccgcttcg ccgacaaggc ccaatgggcc ctctggacgc tctttgcctc
     1141 gctgcacgag caggggacca gtctcggcgg gatcatgttc gacgacatcg gccccaacca
     1201 gcgtcagggg accgcgatct tcgaggattc gttcatcaat cagccgccgc ctaatgactc
     1261 caatccggcg gcctggatcg cgcgcatgaa gttctggacc gcagtccacg agatcggtca
     1321 caccttcaac ctggctcact cctggcaaaa atcgctgggc gtaccctgga ttgcgctcgc
     1381 cgacgagccc gaggcccgca gcttcatgaa ctacccctac gcggtccagg gcggcgagtc
     1441 cgctttcttc agctcgttcg agtaccggtt ctccgaccag gagctgctct tcctgcggca
     1501 tgctcccgaa cgcttcgtcc agcaggggaa cgctctttgg ttcgacaacc acggattcga
     1561 gcagacccgc cgcgctcagc tgccgcggtt gagtctcgag ctccgcgtga accgatcccc
     1621 ggcgacattc gagttcatgg agcccgtcgt catcgagttc aagctgaaga acatctcggg
     1681 ggagcagcag ttcatcgatc agcacctcct ggccaccttc gatcggctga ccgtggtcgt
     1741 gaagaaggaa gggcgtccgg ctcgccagtt ccgcccgttt gcgcagtact gctggcggag
     1801 cgggaagatc gttctccagc ctggggagtc tgcgtacgag tcgctctttg tcggctcggg
     1861 cagcaacggt tgggatgtgg ccgagccagg ccgctacgtc atccaggccg cgttgcatct
     1921 ggtcgaggag gacatcgtct ccacgcctct cgcgatccgc atcgcccctc ccaagggcta
     1981 cgacgaggag tatctggcac aggatctctt caccgaggac gtcgggcggg tactcgcgtt
     2041 cgacggtacc aaagttctgg actcggcgaa taacgtgctg aaagaggtga gcgagaagct
     2101 tccggatagc aaagccgcga tccatgcgaa ggtggcgttg gcgatgccgc tctcgcgtcc
     2161 ctatcgggcg ctcaaggtcg atgccaaggg ctcgccggat gagcagtttg ttttccatgg
     2221 cgacaaggcg aagctgccgg aggcggtcgc cgactttcga agcgctgtcg gccgtcggca
     2281 ggacaagtcg gccgagtcgc tggggcatat cgattacgcc gactatgcca ttcggatgag
     2341 tcaggccgcg agccgcgccg gtgctcgaga ggaggcccgc gagatcggtg aagcgatgct
     2401 cacgacactc aatcgccgag cagttcccga gcgcattctg acacagctcc gggagcggct
     2461 caatgcacct ggggaggagg gaaagtcgaa cgggcggtgg cggaagagcc cgacgcggcc
     2521 tgccaggccg aagtagtacc taagcgtgtc gcgttaaggc cggcccggcg aaagtgccgg
     2581 gctggctccg ctacggcgaa gaaatgcgcc gctcggagcg gtcctagacc ggctcagcgg
     2641 cagtcgtcct cgtccccggc gccgacgcct cgcagcagga cagccacggc cgcgcccccc
     2701 agccgctcgg ccgagagcgt gaggggatcg acaccgcgga agagtcgatg gccgagggcc
     2761 actcccatga tcatataagg gaccaccgcc gcggcagcat cgatatcaca ggacgggatc
     2821 tcgccgagct cgacgccgcg tgccaacgcc tccttcatca tcctccgccc ccgctgcacc
     2881 acctcgtcga acaggatccg cccgacttcg ggaatcttgg gcagctcgtt catcgccagg
     2941 atggcgagct cgatctgccc tggctgctgg taatgcgtcc aggattgcgt cagcaactcc
     3001 cgcaggatct gctcgcgcga ggcagtcgtg ttggcgagca gttcctcccc ggcttcgatg
     3061 tgaggcatca gccgttcacg caggagggcg atgaacaact cgtcctttga gtcgaagtag
     3121 tgatacaccg ctcccttggt caccccggca cgctccgcca catcgttgag cgtcgtccga
     3181 tcgaatccca gctccgagaa cacggcctgg gcggcttcga gtatttcgtc gcgccgggtc
     3241 cccggccgga ggcgacggcg ggagcgcccg tcggcaacgg ccatctcgtc ggttcgcgtc
     3301 agcgggtcgt ctcgcggcgg atcttctttg tatgtcatca gatacctgcc tgcgccgagt
     3361 gctggaggga gctccggaac tttgctgcgg agggtcgggt tactaggtga ccgttctaca
     3421 tacctaccag taggtatgta tcagtcggcg gcccagtagg tcaagagagc ttggcagacc
     3481 cgaaccccaa caggaatgag atagcaatgg taactcgttc gaccgcggtg ggcctactcg
     3541 cgattctgct ccccgcagtg gtatcggccc aaggaccttc ctttgagacc aagaccggcc
     3601 cctcggtgat cgtcttcgcc aacggtggcg gcaactcccc cttaaccaac ctgaaccaga
     3661 ctggcaccgc agacctcaag accggctggg cattgggcgg tggcgcggga gtgcaggtga
     3721 atcagtactt cgcggttcgc ggggtcttcg actttgcccg ctccgagggc agcgcgaact
     3781 cgaccgtgtt cgcgggacag cgcctcaacc actacttcta cggcggcgac gtccagcttc
     3841 gctatccaac ctcgggcggc tttgccccgt acctcctcgc cggtgccggt gcggtcacca
     3901 tcgacaacaa ggacgacctc acatcgaaca ctacgaccag gttcgcgggc aagggcggag
     3961 ttggggttga gtacagcttc ccccgcaatg gtctcggcct cttcgctcag ggcgcatcgt
     4021 acgtctacaa gttcgaccgc gacggcttcg acaagacgca gttcgacgtg ctctggacgg
     4081 ccgggctgag ctaccgcttc ccgttctaaa gcgcgcgttt ttgtgctgcc agagaaacag
     4141 aggaaaccgg cgcaaatgcg cgccgggttg ggttggagca aaacaggcgt tccagcctcg
     4201 tcactgatgg ctcttcgggg cgggcgccct cgggatcgga ggaattgcat ggcagccagg
     4261 gttccggcca cggcggtcgc actgttggga ctcatcag
//
LOCUS       DASRJQ010000265         5644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_674819, whole genome shotgun sequence.
ACCESSION   DASRJQ010000265 DASRJQ010000000
VERSION     DASRJQ010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674819"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..1413)
                     /locus_tag="VFB89_05985"
     CDS             complement(187..1413)
                     /locus_tag="VFB89_05985"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZM26885.1"
                     /translation="MDKVVAVVGNRPILASQVDEEIFSRQSQGLKLPTSPEGIEALRK
                     QVVQGIIDDELLVQEAERDTTIKVTDQEIADGVEEQIKKIRSNFSSEFEYASELRKAG
                     FQTPDEYRRWLTDQQRRAALQNRLVEERRGSGKLKPVIPTEREMREYFDQQKGSLEKR
                     PATISFHQIVIAPQPSAEARQRAYQLADSVAQGLRRGGDFAVAAKRFSMDSGSRELGG
                     SLNWFRRGTMVPEFERVAFALKPGVISDPVETPFGFHVIQVERAQPGEIQARHILIMP
                     EITEQEGDSAHRLAESLLRLARAGASFDSLQRIYHDKSEEREARDVPLNKLPPAYAQA
                     TDTASEGSMVGPFSLSGSGNRLKFTILRITSRRPEGDVRYEDVRDKVRQQLGEQLAVR
                     RYLDTLRKKAYIDVRS"
     gene            complement(1907..3388)
                     /locus_tag="VFB89_05990"
     CDS             complement(1907..3388)
                     /locus_tag="VFB89_05990"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZM26886.1"
                     /translation="MHRLSLALVLGLAACGSFKDTFTSRRDSAAEAGALKLSSDTLAR
                     ILAGPRGLRLTKDAASFVTNLWVDYALFAQASTSGKLPSDSASAAKALWPEVAELRTQ
                     HWHDSLVARRPQPGGTAVDSVYNGNEIRVFQHILIGAPQDAKPEVKAAARKKADATLV
                     KIRKGADFGQLASQLSDDPGSKVDQGFLPPSPKGRFVPSFDSAGWKLNPGETSGIVES
                     PFGFHIIKRPSAEAVQGRLAAFLGQQDAGKADSAYMESLGKTNNLEIAKSAPADMKAA
                     LQNPEASKGSGKRLASFKGGELTVGEYLRWVRALPPQYNAQLKQANDSVLGQFAKVLS
                     LNLLLLRQADSAKVQISGPEWQGLYGRFTGTLDSLQMDMGLAGDSAKKADPGRALNQY
                     FDRLVSGQVRLRPMPGALGTLLREQSRYRINDPGVTHGLELALAQQQKSDSGAAGGTR
                     APGPGLKPAPGGPPVPGPAPSTPPAGKAPGTDSAGKNATGEQK"
     gene            complement(3434..>5644)
                     /gene="mfd"
                     /locus_tag="VFB89_05995"
     CDS             complement(3434..>5644)
                     /gene="mfd"
                     /locus_tag="VFB89_05995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HZM26887.1"
                     /translation="CDNDGQMERLEELLEDRGRPSSAMLAIGALEGGFVMPSLRVLTD
                     HEIFRRARRLRRTRRYRQAAPSAATGTLTVGDYVVHLDHGIGIYRGIATVTVGESVLE
                     VAVVEYEGGDRLNVPLYRLDQLERYRAAGEDGDRPPPRLHRLGGSSWQRVRERTRQAI
                     KQMAAELLDLYARRQVTGGFAFPPDTRWQRELESSFLYEDTPDQRTATEDIKRDMELP
                     KPMDRLLVGDVGYGKTEVAVRAAFKAVQAGKQVAVLVPTTILAEQHGRTFLERLADYP
                     IKIEVLSRFRTAKEQGQVLERLASGEIDIVIGTHRLLSKDVVLKDLGLLIVDEEHRFG
                     VRHKERLKAIRLSVDVLTLTATPIPRTLHLSLSGLRDLTLIETPPRDRSPILTFVEPW
                     DDGLLEEAFARELDRGGQVFAVHNRIETIETIAARVRALSPRARIGVAHGQMPADELE
                     DVMRRFVAGEVDILVSTMIVESGLDVANANTMMVHDAHRFGLAQLYQLRGRVGRSHRR
                     AYCYLLVPDTIDADAEERLKVLEHHTDLGAGYRIALKDLELRGAGNLLGSEQSGHAQA
                     VGFDLYLRWLEETVRALRGQGGIEQPLPPDVVLDRPAHLPDGYVPDDDVKLDLYRRLA
                     RSASSGDIDGLRDEMRERFGPLPTEAETLLDMARLRVTGGSLGLQHVLVRGDEARLTF
                     RAGTQPRLAGLTAALDDVQLAAEVRRTVPLSLRLLRLGGEPMTPALVRALRAVA"
BASE COUNT          947 a         1864 c         1848 g          985 t
ORIGIN      
        1 cggctcagtc agcgcccgcg ccacgatctc ggggccgatc ccgcgggggt caccaagcgt
       61 gattgcgatg cgaggaggca agagagacgg gggaaaggga gaaggggaga agtgggaaag
      121 ggggggagcg ggaaaggaaa ccggagaccc attctcccct tcccccattc tccccttccc
      181 ggccgttcag ctcctcacgt ctatgtacgc cttcttacgc agggtatcga ggtagcgccg
      241 cactgcgagc tgctccccga gctgctgccg gaccttgtcg cgtacgtcct cgtagcgcac
      301 atcgccttcg gggcggcggc tggtaattcg caggatggtg aacttcaacc ggtttccgct
      361 gcccgaaagc gagaacggcc ccaccatcga cccttcgctc gcggtatcgg ttgcctgtgc
      421 gtaggccgga ggaagtttgt tgagcggcac gtcgcgcgct tcacgctctt ccgacttgtc
      481 gtgatagatc cgctgcagcg agtcgaagct cgcgccggcg cgggccagtc gcagaaggct
      541 ttcggccagg cgatgcgcgc tatcgccctc ctgctccgtg atctcgggca tgatcaggat
      601 atgccgggcc tgaatctcgc ccggctgcgc ccgttccacc tggatgacgt ggaagccgaa
      661 gggtgtctcc accggatccg agatgactcc cggcttgagg gcgaacgcca cgcgctcgaa
      721 ctcgggcacc atcgtcccgc ggcggaacca gttgagcgag cctcccagct cgcgggaccc
      781 cgagtccatc gagaagcgct tggccgcgac ggcgaagtcc ccgccgcgtc gcaagccttg
      841 ggcgacggaa tccgccagtt ggtaggctcg ttgacgcgcc tcggctgacg gttgcggagc
      901 gatcacgatc tgatggaacg agatcgttgc ggggcgcttc tcgaggcttc ctttctgctg
      961 gtcgaaatac tcccgcatct cccgctcggt cgggatcaca ggtttgagct tgcctgagcc
     1021 tcggcgctcc tcgaccagtc gattctgcag tgccgcccgc cgttgctgat cagtgagcca
     1081 ccgacgatac tcatcgggcg tctggaatcc ggctttgcgc agctcactgg cgtactcgaa
     1141 ctcggagctg aagttgctcc ggatcttctt gatctgctcc tcgaccccgt cagcgatctc
     1201 ctgatcggtc accttgatcg tggtgtcccg ctccgcctcc tggaccagga gctcgtcgtc
     1261 gatgatgccc tgaaccacct gcttccgaag cgcctcgatc ccctccggtg aggtcggcag
     1321 cttgagtccc tgcgactgcc gcgagaagat ctcttcgtcc acctgtgacg cgaggatggg
     1381 ccggttgccg acgacggcaa cgaccttgtc cactacgaag gtggtatcgc gaagcggtgc
     1441 tcgggccccg gtcggtgccg gctgagcgga ttgcgcggcg gtcgaacccg cagcggtgct
     1501 atcgaaaagg ccgccgcccg gagcgccgac atcctcgacc tgagcaggta tcaggggtgt
     1561 ctcgcccgcg ggagggggag cacctggccg ggcgtttgag ctgggagcgc cgccctgcga
     1621 cggagccgca tttgccggcg tcgtcgcatt tggcggtgct gcggcattag gtgagggagc
     1681 cgggcttact ggcggggctg cgcgctgcga ggccggctgc gtaggcggcg gggcacctgt
     1741 gtcgatcggc gccggcttca ctgccggcgg cgtgaccgct gggggcgcgg ctgccggcgg
     1801 agcagcactg ctatccgtag ggttttgcgc gtgcaggccc ccgctcgaga gagcgaccag
     1861 cattggaacc agcaccagaa cgcgcacgat cgttgccttc aaatgcctac ttctgctcac
     1921 ccgttgcatt cttcccggcc gaatcggtac ccggagcttt gcctgccgga ggcgtgctcg
     1981 gcgccggacc tggaactggc ggccctcccg gcgcgggctt gaggcccggg ccgggggcgc
     2041 gagtcccacc ggccgcgccc gagtcgctct tctgctgctg cgccagcgcc aactccaatc
     2101 cgtgagtcac tccgggatcg ttgatccggt agcggctttg ctctcggagc aaggtcccaa
     2161 gcgcgcctgg catcgggcga agccgaacct gcccggagac gagccggtcg aagtactggt
     2221 tgagcgcgcg gcccgggtcg gccttcttgg cggagtcgcc ggccaggccc atatccatct
     2281 gaagactatc gagggtgccg gtgaaacggc catacagtcc ctgccactcg gggccgctga
     2341 tctgaacctt ggcggagtct gcctggcgca ggagcagaag gttgagggaa agcaccttgg
     2401 caaactggcc gagcaccgag tcattggcct gcttgagctg agcgttgtac tgcggcggca
     2461 atgctcgaac ccaccgcaaa tactcaccta ccgtcagctc gccgcccttg aacgaggcca
     2521 ggcgcttacc cgatcccttg gatgcctccg gattctgcaa ggccgccttc atgtccgccg
     2581 gagcgctctt ggcgatctcg aggttgttcg tcttacccaa gctttccatg taagccgagt
     2641 ctgccttgcc cgcgtcctgt tgcccgagga acgcagccag ccggccctgc actgcctcgg
     2701 ccgagggacg cttaatgatg tggaagccaa agggcgattc cacgattcca ctggtctccc
     2761 cggggttgag cttccacccc gcgctgtcga acgacggaac gaaccgacct ttcgggctgg
     2821 gcggaagaaa gccctggtcc accttgctac cggggtcgtc cgacagctgc gaggccagct
     2881 gaccgaagtc ggcccccttc cggatcttca cgagcgtcgc atcggccttc ttgcgggcgg
     2941 ccgccttgac ctccggcttg gcatcctggg gcgcgccaat cagaatgtgc tggaagaccc
     3001 tgatctcatt cccgttgtac acgctgtcca cggcagtccc gccgggctgg ggacgacggg
     3061 ctaccagcga atcgtgccag tgctgggtgc gcagctccgc gacttcgggc cacaacgcct
     3121 tggccgcgct ggcggaatcc gacggcagct tgccactcgt gctcgcctgc gcgaacagcg
     3181 catagtcgac ccacaagttg gtgacgaagg aggcggcgtc cttggtcagt cgaagcccgc
     3241 gcgggccggc gagaatccgt gccagcgtgt ccgatgaaag cttgagcgct ccagcctcgg
     3301 cggcggagtc acgacgggag gtgaaggtat ccttgaagct cccgcaggca gcgaggccga
     3361 ggaccaaggc tagagacaag cgatgcatga attctccgga gaaaagctat cggctatcag
     3421 ctatcagcgc tgactaggct acggctctca gcgcacgcac cagggccggg gtcatcggct
     3481 cgccgcccag gcgcaggagc cgcagcgaca aaggcaccgt tcggcggact tccgccgcga
     3541 gctggacgtc gtcgagcgcc gcggtgagcc ccgccagacg gggctgcgta cccgcgcgga
     3601 acgtaagccg cgcttcgtcg ccgcgaacca ggacgtgctg caggccaagc gaaccgccgg
     3661 tcacgcgaag tcgcgccatg tcgaggagcg tctccgcttc ggttgggagg ggcccgaagc
     3721 gctcgcgcat ttcatcgcga agcccatcaa tgtcgccaga tgacgcactc cgcgccagtc
     3781 gtcggtagag gtccagcttg acgtcgtcat cgggcacata cccgtccggc aaatgggcgg
     3841 gtcgatcgag caccacatcc ggaggcaacg gttgctcgat cccgccctgc ccgcgcagcg
     3901 cgcgaacggt ctcctccagc catcgcaaat agagatcgaa gcccacggcc tgtgcatggc
     3961 ccgactgctc ggagccgagc aggttgccgg cgccacgaag ctcgaggtcc ttgagcgcga
     4021 tccggtagcc cgcaccgaga tcggtgtggt gctccagcac cttgagccgc tcctctgcat
     4081 cggcgtcgat ggtgtcaggc accagcaggt agcagtaagc ccgacgatgg ctccggccca
     4141 cccgaccacg gagctggtag agctgagcca gcccgaatcg gtgggcgtcg tgcaccatca
     4201 tcgtgttcgc gttagccaca tcgagacccg actcgacgat catggtggag acgaggatgt
     4261 ccacctcccc cgcaacgaat cggcgcatga catcttccag ctcatccgcg ggcatctgcc
     4321 cgtgagccac gccgatgcga gcgcgcgggc tgagcgcgcg aaccctggct gcgatcgtct
     4381 cgatggtctc gatccggtta tgcactgcga atacctggcc gccacgatcc agctcccgcg
     4441 caaaggcctc ttccagcagc ccgtcgtccc atggctcgac gaaggtgaga atcggcgagc
     4501 ggtctcgggg tggagtctcg atgagcgtga gatcgcgcag accggagagc gagagatgaa
     4561 gcgtccgcgg aatcggtgtc gcggtgagcg tgagcacgtc caccgagagg cggatcgcct
     4621 ttagtcgctc tttgtgcctg actccaaagc ggtgctcctc gtctacgatc agaagtccga
     4681 gatccttgag caccacatcc ttggagagca ggcgatgggt tccgatcacg atatcgatct
     4741 caccagaggc gagccgctcc agcacctggc cttgttcttt cgcagtgcgg aatcgtgaaa
     4801 gcacctcgat tttgatcggg taatccgcca gccgctcgag aaaagtgcgg ccgtgctgct
     4861 cggccagaat cgtggtcggg acgagaacgg cgacctgctt gccggcctgt accgccttga
     4921 aggcagcccg caccgcgacc tccgttttgc cgtagcccac atcgcccacc agcagccgat
     4981 ccatcggctt gggcagctcc atgtcgcgct tgatgtcctc cgtggcggtg cgctggtccg
     5041 gcgtgtcctc gtagaggaag ctcgattcca gctctcgctg ccaccgcgtg tctggcggga
     5101 aggcaaaccc acccgtgacc tggcgtcggg cgtagagatc caacagctcg gccgccatct
     5161 gcttgatggc ctggcgggtc cgctcccgga ctcgttgcca actggagcca ccgaggcgat
     5221 gcagtcgcgg aggaggacgg tcgccgtctt cccccgcggc gcgatagcgc tccagctgat
     5281 cgaggcggta gagggggacg ttgagtcggt ctcccccttc atactccacc acggcaacct
     5341 cgagcaccga ttcgcccacg gtgaccgttg cgatgccacg ataaatgccg atgccgtgat
     5401 cgagatggac gacgtagtcg ccgaccgtga gagtgccggt ggcagccgag ggggcggcct
     5461 gacggtagcg ccgagtccgc cgaagccggc gagcgcgccg gaagatctcg tggtcggtga
     5521 ggacccgaag cgagggcatg acgaacccgc cctcgagcgc gccgatcgcg agcatagccg
     5581 aggacggacg accacgatcc tccagcagct cttccaggcg ttccatctga ccgtcgttgt
     5641 cgca
//
LOCUS       DASRJQ010000266         4293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_47906, whole genome shotgun sequence.
ACCESSION   DASRJQ010000266 DASRJQ010000000
VERSION     DASRJQ010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47906"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /locus_tag="VFB89_06000"
     CDS             <1..1090
                     /locus_tag="VFB89_06000"
                     /inference="COORDINATES: protein motif:HMM:NF018452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HZM26888.1"
                     /translation="LGFADLSAGPLVMVMPASGVQGGISDAWQYELHGTAAPGTYLVL
                     GPGQKVDDVTGFIVRQSPTFNIFLGVRLTEEDAAKANAALAQLRMYPYAQRANPPKTD
                     ILDVGTKTWNGLPPRGMEYWERVNDVIQIEPVETRDIFFHAMLRPLGLKKSKPFKPDA
                     RQTKILTDAALVGEAMAKANSADRRFEGARYRADAHWDFALGLDADDPGAFWDLLDER
                     ASWFYEAVGAGAAMAPKHPGPSSAYLSAYRDGAGNWLDGGRSYRLHVPPHPPIEFFWS
                     VTVYDVDTRALIQNQQKIADRSSRMDLGKNPDGSVDIYCGPQAPIGNEKNWIPTVPGR
                     SWFAYFRFYNPTQAYFDRSWPLADFEPL"
     gene            complement(1108..1941)
                     /locus_tag="VFB89_06005"
     CDS             complement(1108..1941)
                     /locus_tag="VFB89_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26889.1"
                     /translation="MRRRYVLVATIVVAVSHAVVTLSHATESAGSGGGDAEDVAKKLS
                     NPVSDIVSVPLQFNWVNGNGPQQDLRFVMNFQPVVPFAISEKWALIGRWIMPYESQPA
                     SLGSATGLGDITASAFFSPRASSGLLWGAGPVFGLPTTSDPALGSGKWSVGPTAVVLK
                     QTGGWSYGMLWNQLWSFASTSNVDRPDVNRGLFQPFLAHVSPKGVTVTLQSEAIANWT
                     ASSSDVWTIPINAQVSKVTHFGPFPLSVAGALGVFVVKPDGGPDWQLRTTFTLVLPGK
                     K"
     gene            2348..3490
                     /locus_tag="VFB89_06010"
     CDS             2348..3490
                     /locus_tag="VFB89_06010"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HZM26890.1"
                     /translation="MLECISQGIIVADRALECLSDAVERTADHVLVTDAHGTILYANP
                     AFERLTGYMRRETYGHTPRLLKSGKQDPSSFRGLWSTLHAGNVFRATFVNRKKSGQLY
                     SSGQTITPIKDGSGNITHFVSVGRDLTERETIADRDGEMRLAGLVQSRLYPPLLQREG
                     IDIAGCALPARATGGDYYDYLNMREGRLGIVVADVSGHGLGAALIMVATRAALRSCAD
                     IPISLDEILDHVNTTLLSDLESGRFVTMCLASLDTRALTLTYSNAGHPSGYVLDRAGE
                     IKYVMESTRIPLGVMSDWNSQPTGTIPLERGDILVFLTDGILEGMGGDGRPFGVENVL
                     ALVKEHRLEPAQQILEQVMDGVQTARGAVPQEDDMTAVICKVEGGP"
     gene            complement(3643..4050)
                     /locus_tag="VFB89_06015"
     CDS             complement(3643..4050)
                     /locus_tag="VFB89_06015"
                     /inference="COORDINATES: protein motif:HMM:NF025589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HZM26891.1"
                     /translation="MSHKITDIEGISTEHARKLEKAGVTTTEHLLAKAFQLKARGKLA
                     LDSGIAEKYLARWTAIADLMRVKGIGPAYSELLFAAGMDSSRKLLTVTPGELIRLMDE
                     QRKAKKLVGGGPKLAEVQQWLIELRAPTFAHAK"
BASE COUNT          790 a         1413 c         1376 g          714 t
ORIGIN      
        1 tctcggcttt gccgatctct ccgcggggcc actggtcatg gtcatgccgg cgagcggcgt
       61 gcagggcggg ataagtgacg cctggcagta cgaacttcac ggaactgccg cgcccggcac
      121 atatctggtg ttgggaccgg gtcagaaggt ggatgatgtc accggcttca tcgtgcgcca
      181 gtcgccgacg ttcaacatct ttcttggcgt acggctgacg gaagaggacg ctgcaaaggc
      241 gaacgcggcc ttggcgcagc tgcggatgta tccctacgcg cagcgcgcca atccgccgaa
      301 gacggatatc ctggacgtag gtacgaaaac ctggaacggg ctgccgccgc gcgggatgga
      361 atattgggag cgcgtcaacg atgttatcca gatcgagccg gtcgagacgc gcgatatttt
      421 cttccacgcg atgttgcggc cgctcggcct gaaaaagagc aaacccttca agccggatgc
      481 gcggcagaca aagatcctga ccgacgcggc actggtcggc gaggcgatgg cgaaggccaa
      541 ctccgcggac cggcgcttcg agggcgccag gtaccgagcc gacgcgcact gggacttcgc
      601 gctggggctg gacgccgacg atcccggcgc attctgggac ctgctcgacg agcgcgcctc
      661 ctggttctac gaggccgtgg gtgccggtgc agcaatggcg cccaagcacc ccggcccttc
      721 atcggcgtat ctcagcgcct accgggacgg cgcgggcaat tggcttgatg gcggcaggtc
      781 gtaccggctt cacgttccgc cccatcctcc gatcgagttc ttctggtcgg tcacggtgta
      841 cgacgtcgac acgcgcgcac tgatccagaa ccagcagaag atcgccgacc gctcgtcgcg
      901 tatggacctg ggcaagaacc cggacggctc ggtggacatc tattgcgggc cccaggcccc
      961 catcggcaac gagaagaact ggattccgac cgtgcccggc aggagctggt tcgcctattt
     1021 ccggttctac aacccgaccc aggcttactt cgatcgatcc tggcccctgg cggatttcga
     1081 gccgctatga ggaggcggca gcaagggcta cttctttccc ggcaggacca acgtgaatgt
     1141 cgtgcggagc tgccagtcgg gtccgccgtc gggcttcacc acgaagactc ccagggcccc
     1201 ggcgacgctg agcggaaatg gcccgaagtg tgtgaccttg ctcacctgag cgttgatcgg
     1261 aatggtccag acgtccgagg acgatgcggt ccaattggcg atggcttcgc tctgcagcgt
     1321 gaccgtcaca cccttgggtg atacgtgtgc gaggaacggc tgaaagagcc cgcggttgac
     1381 gtcgggtcga tcgacattgc tggtgctcgc gaaggaccag agctgattcc atagcatccc
     1441 gtagctccag ccacccgtct gcttgagcac caccgccgtg ggcccgaccg accacttgcc
     1501 cgatccaagg gcggggtcgg aggtggtggg caggccgaat acgggccccg cgccccacag
     1561 caggccgctg gacgcgcgcg gcgagaagaa cgcggaagcc gtgatgtcgc cgaggccggt
     1621 ggcggaaccc aggctcgcgg gctgggactc gtagggcatg atccacctgc caatgagtgc
     1681 ccacttctcg ctgatcgcga agggcaccac cggctggaag ttcatgacga aacgcaggtc
     1741 ctgctgcggc ccgtttccat tcacccagtt gaactgaagc ggaacgctga cgatgtcgga
     1801 caccggattc gagagcttct tggcgacatc ctctgcatcg cccccgcctg agcccgcgga
     1861 ttccgtggcg tgcgacaacg tgaccaccgc gtgagacacc gccaccacaa tcgttgccac
     1921 cagcacatac ctgcgcctca tgggtcgcgc ctcctcagcg agggtcatgg aggccggaat
     1981 ctagcgtggt ctgcggcggg acgcgagacg ctaccaaccc acggccccca gggctcgaac
     2041 tgcggccgaa tgtccttgac ggggccgcca gcctcgccca tgttgggcgc gcacggatga
     2101 tctcgccagg cgggaccgag cacgcacacc gctcggccgg tggaccaccc accgcaaccc
     2161 cgcgggcaat ggactgccag tgactcggtg acggcgaact gctgcggctg aatctgcccc
     2221 ggcacgcgtt ctctccgacg ccgtccaacc accgctcacc ccacaggggg actctctgac
     2281 cggacagcag acttccacga accacacgct ggccagactg ggcgttcaca agcgaagcct
     2341 tcaaggcatg ctcgagtgca tctcgcaagg cattatcgtc gctgatcgcg ccctcgaatg
     2401 cctctccgac gccgtggagc ggacggcgga tcacgtcctg gtcaccgacg cccacggcac
     2461 gatcctgtac gccaatcccg cgtttgaacg cctgacgggt tacatgcggc gcgagacgta
     2521 cggccatacc ccccgactgc tcaagtcggg gaaacaggat ccgtcgtcct tccgtggtct
     2581 gtggtccacc ttgcacgcgg ggaatgtctt tcgggccacg ttcgtcaatc ggaagaagtc
     2641 ggggcagctg tattcctccg gacagacgat cacgcccatc aaggacggct ccggaaacat
     2701 cacgcacttc gtgtccgtgg gcagggacct gacggaacgg gagaccatcg cggatcgaga
     2761 cggcgagatg cgtctggcgg gattggtgca atccaggctg tacccaccgc tcctgcaacg
     2821 cgaggggatc gatattgccg gctgcgcact cccagccagg gccacgggcg gcgactacta
     2881 cgactacctc aacatgcgcg aaggacggct gggcatcgtc gtagcggatg tgagcggtca
     2941 tggtctcggg gcggcgctga tcatggtcgc cacgcgagcg gcgctgcgct cttgcgcgga
     3001 catacccatc agcctggacg agatcctgga tcacgtgaac acgacactcc tgagcgatct
     3061 cgagtcgggc cgcttcgtga ccatgtgcct ggcaagcctg gacacccggg cgctgaccct
     3121 gacgtacagc aacgccggcc atccgtctgg ctatgtgctg gaccgtgccg gcgagatcaa
     3181 gtacgtgatg gaaagcacgc ggatccccct gggcgtcatg tccgattgga acagtcagcc
     3241 caccgggacc atcccactcg agcgtggcga catcctggtc ttcctcaccg acggaatcct
     3301 cgagggcatg gggggcgacg gtcgcccttt cggagtcgag aacgtgcttg cgctcgtcaa
     3361 ggaacatcgt ctcgaaccgg ctcagcagat cctggaacag gtcatggatg gcgttcagac
     3421 cgcaagggga gccgtgcccc aggaggacga catgacggcc gtgatctgca aggtggaggg
     3481 tggaccctga tcaatgcgcg gtgaaaaggc cggacttcct ccgaccgaga cacctggtcc
     3541 ggcgcatgcg cggagggccc tcggagccgg atcggctgcg cagacggaga ccccggccga
     3601 tgaggaccgg ggtcgacgat taggactgtc cggcgccgaa gtctacttcg cgtgagcgaa
     3661 cgtcggggcg cgcagctcga tcaaccactg ctgcacctcg gccagcttcg gacctccacc
     3721 gaccagcttc ttcgccttcc gctgctcgtc catgaggcga atcagctccc cgggcgtcac
     3781 ggtgaggagc ttgcggctgg aatccatacc ggccgcgaac agcagctcgc tgtatgcagg
     3841 accgatgccc ttcacgcgca tgaggtccgc aatcgccgtc caccgggcca ggtacttctc
     3901 ggcaatgccc gaatcgagag ccagcttccc ccgagccttc agctgaaaag ccttcgcgag
     3961 cagatgctcc gtggtcgtga cgcccgcctt ctccagcttc cgagcgtgct ccgtcgaaat
     4021 cccttcgatg tccgtaatct tgtgggacat ggtgtgcccg ttcctctccg gctgacgaga
     4081 agtccccgtg ggcgtgagcg tcgttcgctc gcccccgatc gcgccccaag gcgccggcag
     4141 cgttcccgca tccatcgggc ggaaacgaat ccagtgaggc cgtctcgact gctcgcaacg
     4201 ccgcgccgga ggccgtcgac cgcgtgctca gcaagctgaa aactccgcga cggacacccc
     4261 gacccaggcg gaaggtgaag agggcctgtc agg
//
LOCUS       DASRJQ010000267         2349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_89097, whole genome shotgun sequence.
ACCESSION   DASRJQ010000267 DASRJQ010000000
VERSION     DASRJQ010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89097"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1296)
                     /gene="arcA"
                     /locus_tag="VFB89_06020"
     CDS             complement(67..1296)
                     /gene="arcA"
                     /locus_tag="VFB89_06020"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF002381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HZM26892.1"
                     /translation="MHEFGVHSEVGKLRTVMVCRPGLAHQRLTPGNCRDLLFDDVIWV
                     QEAQKDHYDFVQKMKERDIEVLELHDLLAETLKSKEARSFILDRRVTPNAVGLGTAKV
                     LRPWLDEMPAKKLADHLIGGIAILDLEDSHLKRLLGEAFSGPEFLLPPIPNTLFQRDP
                     SCWIYNGVTINPMFWPARHPETLLQRAVYKFHPMFKGGDFTIWWGDSDEAFENASLEG
                     GDVMPVGKGMVLIGMGERTTRQAVLQVAQSLFKHKAATRVVLCLMPKSRAAMHLDTVF
                     TFCDRDLVTMFREVVDQIRCYTVHETDDHEGYAVKPENKSLLDVVKEALGLPKLRVVE
                     TGGNVYEAEREQWDDGNNVVALEPGVVVAYDRNRYTNTLLRKAGIEVITVRGAELGRG
                     RGGGHCMTCPISRDPAY"
     gene            complement(1342..>2349)
                     /locus_tag="VFB89_06025"
     CDS             complement(1342..>2349)
                     /locus_tag="VFB89_06025"
                     /inference="COORDINATES: protein motif:HMM:NF013444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26893.1"
                     /translation="IIVSNDITAAMMDGPGGVTLTQESIDEAVAFRQTMGRVHREFQK
                     KGEWFFKTWNADQVKEGKGGKSVAFEDASPEFLAREPDAWVLHPGDNWHGFGDLEDGY
                     CMLDPIKVSILTPGVAEKGGLDKRGIPATLVTAYLHRRGVEVEKTTDFTILFLFSIGI
                     TKGKWGTLLNALLDLKRDYDNNVSLAEVLPDLVTGYPDVYGGLGLRDLADQMFAMLKE
                     TRQTHWLAEAFSTLPTPVMTPSAAYQHLVRDEIEHVPLEKLAKRVLATSVVPYPPGIP
                     MLMPGEGTGAADGPYLGYLKALWAWDRRFPGFGHDTHGVENREGTYYVQCLKKGAGGK
                     N"
BASE COUNT          416 a          758 c          714 g          461 t
ORIGIN      
        1 agggaacgat tacgcagatt gaggcccatg ggtccctcta attaggtcga ttgagccgca
       61 ccgtgatcag taggccgggt cacgcgatat gggacaggtc atacaatggc cgcctccgcg
      121 gccgcggccg agctcggctc cgcgaaccgt gatgacctca atgcctgcct ttcggagcag
      181 cgtgttggtg tagcgattgc ggtcataggc cacgacaacg ccaggctcca gcgccaccac
      241 gttgttgcca tcatcccact gttcgcgctc ggcctcgtag acgttgcctc cggtctcgac
      301 gacccgaagt ttgggaaggc cgagggcttc ctttacgacg tcgaggagtg acttgttctc
      361 gggcttgacg gcgtagccct catggtcgtc ggtctcatgg accgtgtagc atcggatctg
      421 gtccacgact tcacggaaca tcgtgaccag atcgcggtcg cagaacgtga acacggtgtc
      481 cagatgcatc gcggcgcggc tcttcggcat gaggcagaga accacgcgtg tcgctgcttt
      541 gtgcttgaag agcgactggg cgacctgcag gaccgcctgg cgggtcgtcc gctcacccat
      601 tccgatcagc accatgccct tgccaacggg catgacatcg ccgccttcca gcgatgcgtt
      661 ctcgaaggcc tcgtccgaat ccccccacca gatggtgaaa tcgccgccct tgaacatggg
      721 gtggaacttg tagacggccc gctgcaagag cgtctccgga tgtcgtgccg gccagaacat
      781 cggattgatg gtgacgccat tgtagatcca gcatgatggg tcccgctgga agagcgtatt
      841 cgggatgggg ggcagaagga attcgggacc gctgaatgcc tcccccagca agcgcttgag
      901 gtggctgtcc tcgagatcca ggatcgcgat cccgccgatc agatgatcgg cgagcttttt
      961 ggcgggcatt tcatccagcc atggcctcaa gaccttggcc gtccccagac ccacagcgtt
     1021 gggtgtcacc cggcgatcga ggatgaagct ccgtgcctcc ttgctcttca gcgtctccgc
     1081 gagcaggtcg tgtagctcca gcacctcaat gtcgcgctcc ttcatcttct gcacgaaatc
     1141 gtagtggtcc ttttgcgcct cctgcaccca gatcacgtcg tcgaacagta ggtcgcggca
     1201 atttccgggc gtcaggcgtt ggtgggcgag gcccggacgg cagaccatga cggtccgcag
     1261 cttgcccacc tcggagtgga cgccaaactc atgcacagct cacctccacg aatctcgact
     1321 cggtgctggc aagccggccg ctcagttctt gccgccggcg cccttcttca ggcactgcac
     1381 gtagtaggta ccttcccggt tctcgactcc atgggtgtca tgcccgaagc cggggaatcg
     1441 gcgatcccag gcccagagcg cctttaagta gcccagatac gggccgtcgg cagcgccggt
     1501 cccttcccca ggcatgagca tcggaatgcc tggcgggtac ggcacgacgc tggtggcgag
     1561 cacccgtttc gccagctttt ccagcggcac gtgctcgatc tcgtctcgca ccaagtgctg
     1621 atacgcagcg ctcggcgtca tgaccggagt cgggagcgtg gagaacgctt cagccagcca
     1681 gtgggtttgc cgcgtttcct tgagcatcgc gaacatctga tccgccagat cgcgaagccc
     1741 aagccctcca tagacatcgg gataacccgt caccagatcc ggcaggacct cggcgagcga
     1801 cacgttgttg tcgtagtcac gcttcaggtc gagcagcgcg ttgagcaagg tcccccactt
     1861 ccccttggtg atgccgatcg aaaagaggaa gaggatggtg aaatcggtag tcttctcgac
     1921 ctcgactccg cggcggtgga ggtatgccgt gaccagcgta gccgggatgc cccgcttgtc
     1981 gagacccccc ttctccgcga cgccaggcgt cagaatggac accttgatcg ggtccagcat
     2041 gcagtacccg tcctcgaggt caccgaaccc gtgccagttg tctccggggt gcaggaccca
     2101 ggcgtcgggc tcccgggcga ggaactcggg agatgcgtcc tcgaacgcga cggacttgcc
     2161 gcccttgccc tccttcacct gatccgcgtt ccaggtcttg aagaaccact cgcccttctt
     2221 ctggaactcg cggtgcaccc ggcccatcgt ctgccggaag gcgaccgcct cgtcgatgga
     2281 ctcctgggtg agggtgaccc cgcccggccc gtccatcatc gccgccgtga tgtcgttgga
     2341 gacgatgat
//
LOCUS       DASRJQ010000268         3228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1068035, whole genome shotgun sequence.
ACCESSION   DASRJQ010000268 DASRJQ010000000
VERSION     DASRJQ010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068035"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VFB89_06030"
     CDS             <1..344
                     /locus_tag="VFB89_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26894.1"
                     /translation="TTTWDLNAGSNVVLVGSFATPAQALASGANFITTQYIKGRVTTG
                     TPTSYTAFTQNGVGTIGTAGGSLTLFTQSTAALAGNRTDNLDLQIDLTGHANLIPGTY
                     TGTLNLQAIVQ"
     gene            366..968
                     /locus_tag="VFB89_06035"
     CDS             366..968
                     /locus_tag="VFB89_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26895.1"
                     /translation="MTLHRPWLRLLLLTALVAVTEEQAAAQAGTVSTARVVSLAATKT
                     GKLTMGVISGAAQTLPALTDNATNNFPTPVVIQTQWDLNPGQTGSVNVIGWFATPSQA
                     LTNGAGTNIPSSRMKGQLTPGAPAPTSYPATFTAFTQNAVGGIGTAGGSLRLFQINIS
                     GSNKTQTRTDNLSLQLDLTGAGVLPPGTYSGTLNIEAVTQ"
     gene            1133..1714
                     /locus_tag="VFB89_06040"
     CDS             1133..1714
                     /locus_tag="VFB89_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26896.1"
                     /translation="MLSARSVPLLAAARMLLVPALFAQTGATSPVRTIAISASKLASV
                     GITLPAGGALTLPGGLSSGINDFTPLPFTTSWSVDPAQTASVAVVAFFEAPARALANG
                     GSAIPSMAILGRVATGTPTGFLPFTQASLTGNGGMVGSSGGSLLLVSEPISPGNARGE
                     RTDQLQLRIDLNGSPPLPAGTYAGTLNLVAVTQ"
     gene            1721..2548
                     /locus_tag="VFB89_06045"
     CDS             1721..2548
                     /locus_tag="VFB89_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26897.1"
                     /translation="MGRTLLQQIGGQRRRTSNRLALGAAILFLPTVLPAQSVSPPIAE
                     YQERARSSVQLSNGSIFPLTVVLDVNGFDVTENGEVVSAPLDTSRVKIKLSSTSFRLA
                     PRSTYNVFYEARADSLPAWFAITAAMSGARTDNGLNLRIILPHVVYLNQKQPLQKTEV
                     VVRRLEVDSAGKAARVQLENLSAHLGRVLELTMTGGKGQSRAAGAFPLLPHHRRWVQV
                     NWDLEQPPERVALRFAKFAIDTTLVPVPVEHPASESARSSDAPSSGLARVPGSADRP"
     gene            2496..>3228
                     /locus_tag="VFB89_06050"
     CDS             2496..>3228
                     /locus_tag="VFB89_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26898.1"
                     /translation="MLHRVDSPEYRAAPTVPESLRRTLLRALLAGALILVALVAGEQA
                     ACAQTVMEVQGGGSSLTGGYGASASFWRNGVDGWVGLGYLDGVRLGAFMRKAIGKDTL
                     RIGNDALVIRYPTDVFGTGYNLLVQGASYARTQGRTGVVAFGGASSEGLAAPAFLAAR
                     AQAPMGALFLQHSVSPTVRLSSTAVIAERQSVVPGIEWQATPDVTASLAAGGSVGRPY
                     GAASLMAHSGRFGFRGSYVYNPSRFR"
BASE COUNT          562 a         1101 c         1029 g          536 t
ORIGIN      
        1 tgacgaccac gtgggacctg aacgccggca gcaatgtggt gctggttggc tcgttcgcca
       61 ccccggctca ggcgctggcg agtggggcca acttcatcac cacccaatac atcaagggtc
      121 gggtgaccac cggtacgccg acgagctaca ccgccttcac ccagaatggc gtcgggacga
      181 tcggtactgc gggcgggagc ctcacgctct tcacgcagag cacggccgcg ttggcaggca
      241 atcggaccga caacctcgat ctccagatcg acctgaccgg tcatgcgaac ctgattcccg
      301 gaacctacac cggcaccctg aatcttcagg cgatcgtgca gtaagcgacg cttaccggcg
      361 ccgctatgac cctccaccgt ccctggctcc gtctgttgct cctcaccgct ctggtagcgg
      421 tgacggagga gcaggcggct gcccaggcgg gaacggtctc aacggcccgg gtcgtctcgc
      481 tggccgctac caagaccggt aagctgacaa tgggagtgat ttcgggcgcc gcccaaacgc
      541 ttcccgcatt gacggacaac gcgacgaaca actttcccac tccggtcgtg attcagaccc
      601 agtgggacct gaatcccgga cagacgggga gcgtgaacgt cataggctgg ttcgccacgc
      661 cgagtcaggc gctgacgaac ggcgcaggca ccaacatccc gtccagccgg atgaaaggac
      721 aactgacgcc gggagcccct gccccgacct cgtacccggc aacgttcacg gctttcaccc
      781 agaacgcggt tgggggaatt ggaaccgccg gcggcagtct gcggctgttc cagatcaaca
      841 tctccggcag caacaagacc cagactcgga cggataacct gagcctgcag cttgatctga
      901 ccggcgcggg cgtgctgccg cccgggacgt acagcgggac cctgaacatc gaagcagtga
      961 cgcagtaggc gcgctggctt gcgcgccgcc caacgctcca agaagggtga gcgggaagtc
     1021 ttagtaagcc tcagcaaggt ggtgcccagg accgaacgcg taatcgatcc ggaggcagcg
     1081 gcagtggaat cccgcgattg gcggaccacc cagccgcgcc gtaggttgga gcatgttgtc
     1141 ggcccgctcg gttcctctcc tcgccgccgc ccgcatgctg ctggtgccgg cgctcttcgc
     1201 gcaaaccggc gcaacctccc cggtccggac gatcgcgatc tcggcgagca agctcgcctc
     1261 ggtcggcatc acgctgccag cagggggtgc tctcactctg cccggtggac tatcgagcgg
     1321 catcaacgac ttcacgccgc ttcccttcac gacttcgtgg agtgtcgacc cggcgcagac
     1381 ggcgagcgtc gcggtcgtcg cgtttttcga ggccccggca cgagccttgg ccaacggcgg
     1441 ctcggcgatt ccctcgatgg caattctggg gcgcgtggcg actgggacgc caacagggtt
     1501 cttgccgttc acccaagcgt cactgaccgg gaacggcggc atggtgggat cgagcggcgg
     1561 gtcactgctc ctggtgtccg aaccgatcag ccccggcaac gcgcggggcg agcggaccga
     1621 ccagctccaa ctgcggatcg acttgaacgg atcgccccct ctgccggccg gcacctatgc
     1681 cggcacgctc aacctggtgg cggttacgca atgaggacct atgggacgca ctctgctgca
     1741 acagatcgga ggccaacgcc gccggactag caaccggctc gccctcggcg ccgctatcct
     1801 gttccttccc acggtcctcc ccgcgcaaag tgtcagtccc ccgatcgccg aatatcagga
     1861 acgcgcccgc tccagcgtcc aactctccaa cggttcgatc ttcccgttga ccgtggtcct
     1921 cgacgtcaac gggttcgatg tcacagagaa cggcgaagtg gtctccgcac cgctggatac
     1981 ctcccgagtc aagattaagc tctcgtccac gagcttccgg ctggccccgc gctcgaccta
     2041 caacgtgttc tacgaggccc gggccgattc gctgccggcc tggtttgcca tcactgccgc
     2101 catgagcggt gcccgtaccg acaacggcct caacctgcgg atcattcttc cccacgtggt
     2161 gtacctgaat cagaagcagc cgctccagaa aaccgaggtc gtggtccggc ggctcgaggt
     2221 agactcggca ggaaaggcgg cgcgggtaca gttggagaat ctgagcgcgc atctgggacg
     2281 ggtgctcgag ctcacaatga ccggcggcaa gggacagagc cgagctgcag gcgcgttccc
     2341 tctccttccc catcaccggc gatgggtcca ggtgaattgg gacctcgaac aaccccctga
     2401 acgagtagct ctccgcttcg ccaagttcgc catcgacacg acgctcgttc cggttccggt
     2461 cgagcatccg gcgagcgaaa gcgccaggag ctccgatgct ccatcgagtg gactcgcccg
     2521 agtaccgggc agcgccgacc gtccctgagt cgctgcgacg gacgttgctg agggcgctgc
     2581 tcgcgggcgc cctcatcctt gtcgccctcg tcgccgggga acaagcggcg tgcgctcaaa
     2641 ccgtcatgga agtccaaggc ggcgggtcat cgctcaccgg cggctacggg gcctccgcga
     2701 gcttctggcg caatggggtc gacggatggg tggggctcgg atatctcgac ggtgttcgcc
     2761 tcggcgcatt catgcgaaag gcgatcggta aggacaccct gcgcatcggc aacgacgcgc
     2821 tggtgattcg gtatcccacg gacgtcttcg gcacgggtta caacctgctg gttcagggcg
     2881 cgagctacgc gcgcacgcag ggtcgcaccg gggtggtggc gttcggcggc gccagctcgg
     2941 aggggctggc agcgcccgcg tttctggccg cgcgcgcgca ggctcccatg ggcgcgctgt
     3001 tccttcagca tagcgtgtcg cccacagtcc ggctgagcag cacggccgtc attgcggagc
     3061 gccagagcgt agtcccgggc atcgagtggc aggccacgcc cgacgtgacc gcctcactag
     3121 ccgccggggg cagcgtgggc cggccttacg gagccgcaag tctgatggcc cattcgggcc
     3181 gcttcggctt tcgtggatcc tatgtctaca acccgagccg cttccgtc
//
LOCUS       DASRJQ010000269         2558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1586805, whole genome shotgun sequence.
ACCESSION   DASRJQ010000269 DASRJQ010000000
VERSION     DASRJQ010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586805"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1240
                     /gene="rho"
                     /locus_tag="VFB89_06055"
     CDS             119..1240
                     /gene="rho"
                     /locus_tag="VFB89_06055"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HZM26899.1"
                     /translation="MLGIFEKAGSNRGFLRRREAGYLPTNGDVHVAERVIREYGLRTG
                     DEIEGETRNTGKGRNHQLERVSGINGRAPEALRHRPEFSRLGATHPSEPLKLECGLQR
                     RGQPDFTNRVIDLICPFGKGQRALVVAPAKAGKTMVLQAIAEGVNANYPAAGLLILLV
                     DERPEEVFEMEAAGFGEVIASSFDNPAAQHVAVAELVLERARRRVELGEDVVLIVDSL
                     TRLARAYNTVEKGSGRTLTGGLDAQSLEKPKRFLGSARAVEPSQGGGSLTIIATALVD
                     TGSRMDQVIFEEFKGTGNSELVLSRELADRRIFPAIDLSASGTRREELLLTQDELAAS
                     RLLRQRIAVMSPVNAMTDLLDEMRRDRTNADLVRRLSAS"
     gene            complement(1256..>2558)
                     /locus_tag="VFB89_06060"
     CDS             complement(1256..>2558)
                     /locus_tag="VFB89_06060"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZM26900.1"
                     /translation="GSGARKYSTDSPAKLPNLWNLYSLRQSPYFQDTLGGEAEHHPLS
                     LFVGRQPELETLISNIGSGGSSRQVVGGRPGVGKTTLVQGVKAAAVGAGFWATDSLVT
                     FYPEDTTELLLGRLLGGIYDAIVTARPHTAEQPAMQAAQQLVRVARLATGGLNVSAAG
                     IGVGVSRGVAMATPAGALLLDGPRLVRELLELVLAAGARGIVLHLNNLEILSERDAAA
                     AADILRSLRDPVLLQPGLHLILAGTTDAVTAVVTRHPQVRSVFASPLLLEPLPPADVH
                     ALLAARYAHLRLEPDREPVAPIAPEAVAGLYAFFQGDLRGLFKALEEGATLLMGVGAS
                     PGTPLALDDLRPALQRRYEAELTAALEPVRAEQLRAWAAAGVSVPQTQEKLRGIWHLS
                     QPATSNAVRDLIERGYAISLPRRGAAPIEYLLTGASRLIFG"
BASE COUNT          486 a          811 c          835 g          426 t
ORIGIN      
        1 gtccgatgca gcgtcgtgca ccggggtgcc atgctgccac ggcgcacggt atcttcccga
       61 aacctccttc gccgatcacc atgtgtgagt ccataatcgc cttcgagagg ccggcgtgat
      121 gctgggcatc ttcgagaagg ccggatcaaa tcgcggattt ctacgtcgtc gcgaagcggg
      181 atacctccca accaacggcg acgtacacgt ggccgagcgg gtgatccgcg agtatgggct
      241 gcgcacgggt gacgagatcg aaggcgagac ccgcaatacg ggcaaggggc gtaaccatca
      301 gctcgagcgg gtatccggta tcaatggtcg cgcgccggaa gctctgcgcc atcgacccga
      361 attcagcagg ttaggagcga cgcatcccag cgagccgctc aagctcgagt gtggcttgca
      421 gcgacgtggt cagcccgact ttaccaatcg ggtcatcgat ctgatctgtc cattcgggaa
      481 gggacaacgc gcgctggtcg tggccccggc caaggccggg aagacgatgg tgctgcaggc
      541 catcgctgaa ggcgtcaacg cgaactaccc cgctgcagga ctgctcattc ttctggtgga
      601 cgaacgaccg gaagaagtat tcgaaatgga ggccgccgga tttggtgaag tcattgcctc
      661 gagcttcgac aatccggctg cgcaacacgt cgcggtggcg gagctggtct tggaacgggc
      721 acggcgccga gtcgagttgg gcgaggacgt ggtgctgatc gtcgattctc tgacccgcct
      781 ggcgcgggcg tacaacacgg tcgagaaagg aagcggcagg acgctcaccg gggggctcga
      841 cgcccagtcg ctggaaaaac ccaagcgatt tctgggcagt gctcgagcgg tcgagccatc
      901 gcaaggaggc ggctcgctca ccatcatcgc caccgcgctg gtagacaccg gcagccggat
      961 ggatcaggtc atcttcgagg agttcaaggg caccgggaac agcgagctgg tgctcagtcg
     1021 ggagttggca gaccgccgca tcttccccgc catcgacttg agcgcgagcg gcacccgccg
     1081 ggaagagctg ctcttgactc aggacgagct tgcggcgtcc cggctcctcc gacagcgcat
     1141 tgcggtgatg tcccccgtga atgcgatgac cgacctgctg gacgaaatgc gccgggatcg
     1201 gaccaacgcc gatctggtaa ggcgactcag cgcttcctaa ccctggacct tcgatctacc
     1261 cgaaaatcag gcgactcgcg ccggtcagga gatactcgat cggtgccgcg cctcgccgcg
     1321 ggagcgaaat cgcataccct ctctcgatca gatctcgaac cgcattcgac gtagccggct
     1381 ggctcaagtg ccaaatcccc cgaagctttt cctgggtctg agggaccgag actccggccg
     1441 ctgcccacgc gcggagctgc tcggcgcgca ccggttcgag ggcggccgta agctcggcct
     1501 catatcggcg ctgcagcgca ggtcggaggt cgtccagcgc gagcggcgtt cccggacttg
     1561 ctcctactcc catcagcaac gttgctcctt cctccagtgc cttgaacagc ccgcggaggt
     1621 caccctggaa gaaggcgtac aacccggcga ccgcctctgg cgcgatcggc gcgacgggct
     1681 cgcgatccgg ctcgagccga agatgggcat agcgcgcagc aagcagtgca tgcacgtcgg
     1741 cgggaggcaa cggctccagc agcaatggac tggcgaagac tgaccgcact tgcgggtgac
     1801 gggtcactac cgcggtcacg gcgtccgtag tgccggcgag aatgaggtgc aaacccggct
     1861 ggagtaacac cggatcgcgg agactgcgaa gaatatccgc cgccgcagct gcgtcgcgtt
     1921 cgctcaaaat ctccagattg ttgagatgca gaacgattcc gcgcgcgccg gcagccagga
     1981 ccagctcgag cagctcgcga actaatcgag gcccatccag caggagtgcg ccggccggtg
     2041 tcgccatggc gacaccgcga gaaacaccca cgccgatacc cgccgctgaa acgttcagac
     2101 ctccggtcgc gagtcgggca accctcacca gctgttgcgc tgcctgcatc gcgggctgtt
     2161 ccgccgtatg cggccgggct gtgacgatag cgtcgtagat cccgccgagc agccgtccga
     2221 gcaggagctc ggtcgtatcc tcgggataga aagtcaccag actgtcggta gcccagaagc
     2281 cggccccgac ggccgcggct ttgacgccct gcaccagcgt ggtctttccg accccgggtc
     2341 ggccgcccac cacctgcctc gatgagccgc cgctcccgat gttgctgata agggtttcga
     2401 gttccggctg gcgcccgaca aagaggctca gcggatggtg ttccgcttca ccaccgagag
     2461 tgtcctggaa gtagggtgac tggcgtaagc tgtagaggtt ccagaggtta gggagcttgg
     2521 ctggcgaatc ggtggagtat ttccgagccc cgctgcca
//
LOCUS       DASRJQ010000270         2192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2303312, whole genome shotgun sequence.
ACCESSION   DASRJQ010000270 DASRJQ010000000
VERSION     DASRJQ010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2303312"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VFB89_06065"
     CDS             complement(<1..453)
                     /locus_tag="VFB89_06065"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZM26901.1"
                     /translation="MIGPAYLGPGDLLAGRYEIVRELGRGGFSIVYLATDRSLSTPVA
                     IKLLVPPPASAQMARERLRREVQAVRGLVHSNIVGVHDFLEEGAWSFVVMEYVEGPDL
                     AVRVRDRGPLQPDDAARLGTDVAAALAAAHRHGILHRDVKPQNILLDPD"
     gene            complement(450..1043)
                     /locus_tag="VFB89_06070"
     CDS             complement(450..1043)
                     /locus_tag="VFB89_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26902.1"
                     /translation="MLRFLYLAGIGYLGICLLAIGVGRAGGPVHSGTGAGVALAVPPG
                     RGDGGTWFAGIKPFCNSVEVETAQQRNPPPGGADGQGYSAACYALAGRIEQARTVIER
                     LSGDDRSYAASIVFGVGHPVADAGDDKSAGPIMELVIEFQPDNYMALYHAGMSEYVLG
                     QPDLAEQHLRRFLELYHANDGWRTNAVEVLQRLSGGP"
     gene            complement(1110..>2192)
                     /gene="fahA"
                     /locus_tag="VFB89_06075"
     CDS             complement(1110..>2192)
                     /gene="fahA"
                     /locus_tag="VFB89_06075"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HZM26903.1"
                     /translation="LNGFLAMGSRYWSALRRAVSRLLAVDAAGPLLDPRAGPRLLAPA
                     SEVELLLPVMIGDYTDFYASLHHATNVGSMLRPDNPLFPNYKWLPIGYHGRASSIVPS
                     GTPIRRPQGQTRDGNAEAPTFGPSRRLDYELEVGFFVGPGNPLGEPIRIADASRHLFG
                     ICLLNDWSARDLQAWEYQPLGPFLAKNFATSISPWIVTLDALAPFRSPFKRAAGEPEP
                     LRYLQDEDDSRAGVIDLSLEVLLRSSRMREQGVAPASVSVSRLPDLYWTPAQMLAHHA
                     SSGCNLRPGDLFASGTVSGPAKESRGCLLELTWRGAEPLTLPTGEERRFLEDGDEVIL
                     RGWCEREGFVRIGLGECRGEVKGSSA"
BASE COUNT          406 a          723 c          732 g          331 t
ORIGIN      
        1 gtcggggtcg agcaggatgt tctgcggctt gacgtcacgg tggagaatgc cgtgccgatg
       61 ggccgcggcc agtgcggcgg caacgtcggt accgagccgg gcggcgtcgt ccggctggag
      121 aggtccacga tcgcgaaccc gcacggccag atccggcccc tccacgtact ccatgaccac
      181 aaagctccag gcgccctctt ctaggaaatc atgtacgcct acgatgttgc tgtgaaccag
      241 accgcggacc gcctggactt cgcggcggag ccgctcgcgg gccatttgcg cgctcgcggg
      301 tggaggcacc agcagcttga tggcgacggg agtgctgaga ctgcgatcgg tggcgagata
      361 gacgatcgaa aagccgcccc gcccgagctc gcgcacgatc tcgtagcgac cggcgagcag
      421 atcgcccggc ccgaggtagg cgggaccgat catggcccgc cggaaagtcg ctgaaggacc
      481 tcgacggcgt tggttcgcca gccgtcattg gcgtgataca gctcgagaaa acgccggagg
      541 tgctgctcgg ccaggtcggg ttggccgagc acgtactcgg acattccggc gtgatagagg
      601 gccatgtagt tgtcgggctg gaactcgatg accagctcca tgatgggccc ggccgacttg
      661 tcgtcgccgg cgtcggcgac ggggtgcccg accccgaaga ctatcgacgc agcataggaa
      721 cgatcgtcgc ccgagagacg ctctatgacg gtgcgggcct gctcgatccg accggcgagc
      781 gcgtagcagg cagcgctgta gccctggccg tcagcgccgc cgggaggggg atttcgctgc
      841 tgggccgttt ccacttcgac cgaattgcag aatggcttga tccccgcgaa ccaggtgccg
      901 ccatctccgc gaccgggagg cacggcaaga gcgacgccag cgccggtgcc tgaatgcacg
      961 gggccgccgg cgcggccgac tccaatcgcc aggaggcaga tccccaaata ccctatgcct
     1021 gcgagataga ggaagcgcag catggggccc gctagctcct gtggtgagag gtaagaggga
     1081 gacgttgcaa gaatcgcgca agtacacgct caggcgctgc tccccttcac ctccccccgg
     1141 cactcaccca acccgatccg cacaaagcct tcccgctcgc accagccccg caagatcacc
     1201 tcgtcgccgt cctccaggaa ccgacgctcc tcgccggttg gcagagtgag aggctccgct
     1261 ccgcgccagg tcagctcgag cagacaacca cgcgactcct tcgcgggtcc cgaaacggtc
     1321 ccgctcgcga acaggtcgcc cggtcgaaga ttgcaaccgc tcgacgcatg gtgcgcgagc
     1381 atctgggccg gcgtccagta aagatcgggt aggcgactca cgctcaccga ggcaggcgcc
     1441 acgccctgct ctcgcatacg ggacgagcgg agcagaacct cgagcgacag atcgataacg
     1501 ccggctcgag aatcgtcctc gtcctgcaag tagcgtagcg gttcgggctc gccggccgct
     1561 cgcttgaacg ggcttcgaaa cggcgcgagg gcatccagcg tcacgatcca gggagagatc
     1621 gaggtcgcaa aattcttggc gagaaacggc ccgagcggct gatactccca ggcttgcagg
     1681 tcgcgcgcgg accagtcatt cagcaggcag atgccgaaga gatgacgtga cgcgtccgcg
     1741 atgcggatcg gttcgcccag cggattgcca ggcccgacga agaaccccac ctcgagctcg
     1801 taatcgagcc ggcgcgacgg gccgaacgtt ggcgcctcgg cgttgccgtc ccgagtctgc
     1861 ccttgggggc gacggatcgg cgttccactg ggcacgattg aagaggctcg tccgtgatat
     1921 ccgatcggca gccacttgta gttgggaaac agcggattgt ccggacgcag catgcttccc
     1981 acgttggtcg cgtgatgcag cgacgcgtaa aagtcggtgt agtccccgat catgaccggg
     2041 agcagcagct ccacctccga cgccggtgcg agcagccggg gccccgctcg cgggtcgagc
     2101 agtggtccgg ctgcatccac tgccagcagc cggctgaccg cgcgccggag cgccgaccag
     2161 tatcgactgc ccatggccaa gaatccgttc ag
//
LOCUS       DASRJQ010000271         7359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3512344, whole genome shotgun sequence.
ACCESSION   DASRJQ010000271 DASRJQ010000000
VERSION     DASRJQ010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7359
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3512344"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="VFB89_06080"
     CDS             complement(<1..930)
                     /locus_tag="VFB89_06080"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZM26904.1"
                     /translation="MGSLVLRAIEILFVLHAREATVAGVVRDEETSAPLVGAVVQVTD
                     LDRTAATDADGRYVLRGIPAGPHSLTIRFLGYEPRTVLALVPADGQLEINVSLRREPV
                     RLPTLEARAPAIVRGLDRGDSTPFPDRASSIAAVRNDPQLSEPDVFQALDGGEVVLSP
                     ESPSGIHVRGGASDQTSYLLDGIPVLSPYHSAGLFSAWNPDALAGIQLSSSAPSPTYP
                     DALSGTVSAVTRQPGATVRTQGSITTSQARLTVDGPIGVGHAGYLLSLRSGLPNAFAP
                     RGEASYPDADTGDWLATLEAPALGGWMRLLGYNS"
     gene            1132..2121
                     /locus_tag="VFB89_06085"
     CDS             1132..2121
                     /locus_tag="VFB89_06085"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HZM26905.1"
                     /translation="MRADLLLLAADLVRRGEPFALATVVRRQAPSSAREGDTALITTG
                     GTCHGWVGGTCTQSGVIHEALRALRDGTPRLVVFSADPDVIARTDAIVVPMTCESGGA
                     VEIHIEPVVPAPRLVVFGISPVAQATARIAKAAGHSVDAVDPSADRATFPEADHVITD
                     AAALEPTLPMPYRGDRTFAIVATMGRWDVEATLAALSLEPAYLGLVASQRRFTELRDA
                     LRAKGISAHAIKRIKNPAGLDIGARTPEEIALSILAEIVQLGRSGQRMPVAGLEVAKG
                     ERDPVCGMAVVVATAKHSAEFAGRTYYFCCGSCRERFLAAPDRYAVAEAGHTR"
     gene            2153..3046
                     /locus_tag="VFB89_06090"
     CDS             2153..3046
                     /locus_tag="VFB89_06090"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZM26906.1"
                     /translation="MRPEIRALQDALAEQGYIAEPAIATALFLAREMKKPLLIEGDAG
                     VGKTEVAKVLAQVLGTELIRLQCYEGLDVNTALYEWNYQRQLVRLRIAEQNGQDLGKL
                     EEMIFGREYLLERPLLRAITRREGPAVLLVDEIDRADEGFEAFLLEVLSDFQVTIPEL
                     GTIRAQHVPYVVLTSNRTREIGDAVRRRCLYLDIPHPSLEKEVRIIHTKVPGVSERLA
                     DQIARFAQALRVRRLARSPGVAETIDWAEALTRLHQDRLDLATVRETLGCVVKDRRDV
                     DELTDAELSALIAQAAETTAG"
     gene            3043..4239
                     /locus_tag="VFB89_06095"
     CDS             3043..4239
                     /locus_tag="VFB89_06095"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZM26907.1"
                     /translation="MSERDLVVHLAGFAGALRAGGVRVSLSDELDAAQALTLVDLLDR
                     DEVRRSLLISLRIRPGDRAWFDDLFDRFWVEGGSPRHPAAAMMRVVETRHPSAVERAR
                     QMTTAPASAPDEATTAGESDQANDGAPGYSPDALLRKKPFDECTREELNEMEMLLARL
                     SLKLATRRSRRLIPTRGRGTVDLRRSFRRAIGTGGELRWLARRAHAIDRPSLVVLCDT
                     SGSMDSHARFLLTFMLSLRRVARRIELFAFNTALTRLTPLLTGGHTRGGSATIEGDAQ
                     SRVSSVLARLAAEVPDWSGGTRLGECLTEFVERYGSQLVDSTATVVIVSDGLDRGDPD
                     LLVRAMRAIQSRAWKVIWLNPLLGDSRYQPLARGMQAALPFVDRFAPAHNLESLERFI
                     THLEVA"
     gene            4236..5111
                     /locus_tag="VFB89_06100"
     CDS             4236..5111
                     /locus_tag="VFB89_06100"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HZM26908.1"
                     /translation="MIPAAFEYHRAANLADALALLERHGDGAKVLAGGQSLLPLLKLR
                     LGMVTDLVDIGAVEGLDYIKEEGGFLKIGAMTREVELERSDLIKAKYPILLDTAAVIA
                     DPLVRNRATVGGNLAHADPANDHPATMIALGATVVARGPKGERTIPIEQFFKDVFTTA
                     LAPNEILTEIRIPVPGPRTGGAYVKLERKVGDFAIAGAAAQVTLRDSGEVERAGIGLT
                     DAGKVALKAGEAEKYLVGKKPVAPTLGEAGKLAGQAATPTADRRGSVQYKREMARVLT
                     MRALQKAVERAGGRR"
     gene            5111..5593
                     /locus_tag="VFB89_06105"
     CDS             5111..5593
                     /locus_tag="VFB89_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZM26909.1"
                     /translation="MSRHQIRVTVNGTSYEVEVESRLLLVHLIRDQLRLTGTHIGCDT
                     THCGACTVLLTGPDGTPEPVKSCTVLAVQADGREVTTVEGLEKDGKLDPIQEGFWQEH
                     GLQCGFCTPGMMLTSVALLKRNPRPSEADIRHAISGNLCRCTGYVNIVKAVQYAAAKR
                     "
     gene            5612..>7359
                     /locus_tag="VFB89_06110"
     CDS             5612..>7359
                     /locus_tag="VFB89_06110"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZM26910.1"
                     /translation="MTLRTSAEVSGMGHSMKRKEDPRFIRGKGTYVDDITLPGMVYLD
                     IVRSPLAHAKIVSINTENTLKIPGVLAVITGQTLAQFNLHWMPTLMSDTQMVLPVDKV
                     MYQAQEVAAVVATDRYAAADGVAAVDVDYEPLPVVVDPFKALEPGAPVLRTDKKDKKD
                     NRIFHWEAGDKEATDKAFAAADVVVKQSLYIPRIHVASIETCGCVADFDRVRGKLTVY
                     MTTQAPHAIRTVFALVAGHVGLSEEKIRIISPDIGGGFGGKVPVYPGYVIAVAASVVL
                     GRPVKWIEDRMENLQADSFARDYHVTAELAAKKDGRMTALRIKTVADHGYADAACNPS
                     KFPAGLFSICTGSYDLKNAFAEVDGVYTNKPPGGIAYRCSFRVTEAVHTIERITDVLA
                     QQLGMDPAELRLKNFIPKERFPYKSALGWEYDSGDYTTALKKAMEIVDYPALRKEQAE
                     KRKRGELMGIGIASFTEIVGAGPSKHFDILGLKMFDSCEIRVHPTGKAVARFGTKSQG
                     QGHETTYAQIVAEELGIPAAHVEVEEGDTDTAPYGLGTYASRSTPTAGAAGAMAARKI
                     RDKAKKIAAHLLEVSE"
BASE COUNT         1347 a         2324 c         2493 g         1195 t
ORIGIN      
        1 gctgttgtag ccgagcagtc gcatccatcc gcccaacgcc ggtgcctcga gtgtcgcgag
       61 ccagtcaccg gtgtcggcat ccgggtacga agcttcgccc ctcggtgcaa atgcgttcgg
      121 aagaccggac cgcaggctca gcaggtagcc ggcgtgccct acgccgatcg gtccatcgac
      181 ggtgaggcgc gcttgactgg tagtgatgct tccctgcgta cgcacggtgg cgcccggctg
      241 gcgggtaact gccgaaacgg tgcccgaaag agcatccgga tacgttgggg aaggtgcgga
      301 ggaggacagc tgaatcccgg ccagcgcgtc ggggttccag gcgctgaaca agccggccga
      361 gtgatacgga ctcaggacgg gaattccgtc gagcaggtag gaggtctggt cggaagcgcc
      421 tccgcgaacg tgaattccgc tgggagactc cgggctcaag accacctcgc ctccgtcgag
      481 cgcctgaaac acgtcgggct cggacagttg cggatcatta cggacagccg cgatcgagct
      541 cgcgcggtcg gggaacggag tgctatctcc ccgatcgagg ccgcgaacaa tggcaggtgc
      601 tcgggcctcg agcgtgggaa gccgaacggg ctcgcggcgg agggagacgt tgatttcgag
      661 ctggccgtcg gccgggacga gggcgagaac cgtgcgcggt tcgtagccaa ggaagcgaat
      721 cgtcagcgag tgagggccgg cgggaatgcc acgcagcacg taacgcccat cggcatcggt
      781 ggcggcggtg cggtccagat cggtcacttg aaccaccgcg cccaccagcg gcgcggaggt
      841 ctcctcgtcc cgcaccacgc cggccaccgt cgcctctcgg gcgtgaagga cgaacaggat
      901 ctcgatcgcg cgaagaacca acgaccccat cggtgtctcc tgggtgcccc gcctcgtttc
      961 ccgataagaa gggcctcgga cgcgaagtgc gcaagcgtag tctggcctac ctcattgggg
     1021 cgccgtatct tggcggcggg acccgtagga cctgcggagt ttccattgtg aacggtcccg
     1081 atggagcaca agagcatgcc ggctcgacta cctcggagca ataactaggc gatgcgcgcc
     1141 gatctccttc ttctcgcagc cgacctggtc cgacgtggtg agccgttcgc cctcgctacg
     1201 gtcgtgcgcc ggcaggcacc cagctctgcg cgcgagggcg ataccgcgct gatcacgacc
     1261 gggggcacct gccacggatg ggtcggcggc acctgcaccc agtccggcgt gattcacgaa
     1321 gctctgcgcg cgctgcgcga cggaactccc cgcctggtcg tcttctctgc agatccggac
     1381 gtcattgccc gcaccgatgc catcgtcgtt ccgatgactt gtgagagtgg aggcgctgtg
     1441 gagatccaca tcgagcccgt ggtacctgcg ccaaggctcg tagtcttcgg tatctcacct
     1501 gtggctcaag ccacagcccg catcgccaag gccgcaggcc attcggtaga tgcagtcgac
     1561 cccagtgccg accgggcaac gtttccggaa gccgatcacg tgatcaccga cgctgccgcg
     1621 ctggagccca cgttgccgat gccctatcga ggggatcgca ccttcgccat cgtcgccacc
     1681 atgggtcgct gggacgtcga ggcgacgctg gcggcgctct ccctcgagcc ggcgtacctg
     1741 ggtttggtgg caagtcagcg ccgcttcacc gagctgcggg acgctctccg cgcgaagggc
     1801 atctcggcac acgcgatcaa gcggatcaag aatccggcgg gtctcgacat cggtgcccgc
     1861 acgcctgaag agatcgccct cagcattctc gccgagatcg tgcagctggg ccgatccggc
     1921 cagcggatgc cggtggcagg gctcgaggtc gcgaagggtg agcgtgatcc ggtctgcggt
     1981 atggccgtcg tcgtggcaac cgcgaagcac agcgccgagt tcgccgggcg cacgtactac
     2041 ttttgctgcg gcagctgtcg ggagcggttt ctcgccgctc ctgatcgtta cgccgtcgcc
     2101 gaagccggac acacgcggtg aacgacgcga ccggcggcgg ctgggggagg gcgtgagacc
     2161 cgagatccgc gcgctgcagg acgcgctggc cgagcagggg tacatcgccg agccggcgat
     2221 tgcgaccgcc ctgttcctgg cgcgcgagat gaagaagccg ctcctgatcg agggcgatgc
     2281 cggggtgggg aagaccgaag tcgccaaagt actcgcccaa gtgctcggca ccgagctgat
     2341 tcggctccag tgttacgaag gactcgacgt caacaccgcg ctgtacgagt ggaactatca
     2401 gcggcagctg gtccggctcc ggatcgccga gcagaatggc caggatctgg gcaagctcga
     2461 agagatgatc tttgggcgcg agtacctcct cgagcggccg ctactccgtg cgatcacccg
     2521 gcgcgaaggt cccgcggttc tcctggtgga cgagatcgat cgggcggacg agggattcga
     2581 ggccttcctg ctcgaggtct tgtcggattt ccaggtcacc atccccgagc tgggcaccat
     2641 ccgggcgcag catgtgccct acgtggtgct cacctccaac cggacccgcg agatcggcga
     2701 cgcagtgcgc cgccgctgtc tctatctcga cattccgcat ccgagcctgg agaaagaagt
     2761 gcggatcatt cacaccaagg tgccgggcgt gagcgagcga ctggcggatc agatcgcgcg
     2821 cttcgcgcag gcgctccggg tccggcggct ggcgcgctcg ccgggcgtgg ccgagacgat
     2881 cgactgggcc gaggcgctga ctcgcctgca ccaggaccgg ttggatctcg cgacggtgcg
     2941 ggagacactg ggttgcgtgg tcaaagaccg gcgcgacgtg gacgagctga ccgacgccga
     3001 attgtccgcg ctgatcgcgc aggccgcgga gacgacggcc ggttgagcga gcgtgacctg
     3061 gtagtgcatc tggccggctt tgccggtgca ctccgggcgg gcggggtacg agtaagcctg
     3121 agcgacgagc tggatgccgc gcaggcgctc acgctggtgg acttgctgga ccgcgatgaa
     3181 gtgcggcgct cgctgctgat atcgctcaga attcgccccg gagaccgcgc gtggttcgac
     3241 gacctgttcg accgcttctg ggtcgagggc ggttcgcctc gacatcccgc ggctgcaatg
     3301 atgcgggttg tagagaccag gcatccttcc gctgtcgagc gcgcgcgcca aatgacaact
     3361 gcccccgcga gtgcgcctga cgaggcgacc acggcaggtg agtcggatca ggcgaatgac
     3421 ggtgcacccg gctatagccc cgacgcgctg ctgcgaaaga aaccgttcga cgagtgcact
     3481 cgcgaagagc tgaacgagat ggaaatgctg ctcgccaggc tttcgctcaa gctcgctacc
     3541 cgccgttccc gccggctcat cccgactcga ggccggggca cggtcgatct tcgccgcagc
     3601 ttccgccgag cgatcggcac cggcggcgag ctcagatggc tggctcggcg cgcgcacgcg
     3661 atcgaccggc ccagtctggt cgtgctgtgc gacacgagcg gctcgatgga cagccatgcg
     3721 cgctttctgc tcaccttcat gctctcgctc cggcgagtag caagacgcat cgagctgttc
     3781 gcattcaaca cggcgctcac tcgactcaca ccattgctca ccggcggaca cacgaggggc
     3841 gggtccgcca ccatagaagg cgacgcgcaa agcagggtga gcagcgttct cgcccggctc
     3901 gcggcagagg tgcccgactg gtccggcggt acccggctgg gcgaatgtct gacggagttc
     3961 gtcgaacgat atggaagcca gctggtcgat tcgacggcga ccgtagtcat cgtgagcgac
     4021 gggctggatc gcggcgatcc ggacctcctg gttcgggcca tgcgcgcaat tcagtctcgc
     4081 gcctggaagg tcatctggct caacccgcta ctgggcgact cccgctatca gccgctcgcg
     4141 cgcgggatgc aggcagcgct gccgttcgtc gaccgctttg cgccggcgca caatctggaa
     4201 tcgttggagc gattcatcac acatctggag gttgcatgat accagcggcc ttcgagtatc
     4261 accgtgccgc caacctggcc gacgcgctgg cgctgctcga gcgccatggg gacggggcca
     4321 aggtgctggc cggaggccag agcctcctgc cattgctcaa gttacgcctg ggtatggtca
     4381 ccgatctggt ggacatcggg gcggtcgagg gactggacta tataaaggaa gagggaggct
     4441 tcctgaaaat cggggcgatg acgcgggagg tcgagctgga gcgctccgat ctgatcaaag
     4501 ccaagtaccc aattttgctc gacaccgccg ccgtcatcgc cgacccgctg gtccgaaatc
     4561 gagccaccgt gggtggcaat ctggcccacg ccgaccccgc caatgatcat ccggccacca
     4621 tgatcgcgct cggcgcgact gtggtcgcac ggggaccaaa gggtgagcgc accatcccga
     4681 tcgagcaatt cttcaaggac gtcttcacca ccgctctcgc ccccaacgaa atcctcaccg
     4741 aaatccggat tccggttccc gggccgagaa caggtggcgc gtacgtcaag ctcgagcgaa
     4801 aggtgggcga cttcgccatt gccggagcgg cggcgcaggt cacgctgcgg gacagtggcg
     4861 aagtggagcg ggcgggcatc gggcttacgg atgcggggaa ggtggcgctc aaggcggggg
     4921 aagcggagaa atatctggtc ggcaagaagc cggtcgctcc aacgcttggg gaagcgggga
     4981 agctggcggg acaggcggcg acgccgacgg ccgatcgtcg gggctcggtg cagtacaagc
     5041 gggagatggc ccgggtgctc acaatgcgcg cgctgcagaa ggccgtagag cgggcgggag
     5101 gacgacgctg atgtcgagac accagatccg tgtcaccgtg aacgggacca gttacgaagt
     5161 cgaggtcgag tcgcgcctcc ttctggtcca cctgattcgc gaccagcttc gactcaccgg
     5221 gacccacatt ggctgcgata ccactcactg tggtgcttgc accgttctcc tgaccggacc
     5281 agacggcacg ccggaaccgg tcaagtcgtg caccgtgctc gcggtgcagg cggatgggcg
     5341 cgaggtgacc acggtcgagg gcctggaaaa agacggcaag ctcgacccga tccaggaagg
     5401 cttctggcag gagcacggac tccagtgcgg cttctgcacc ccaggcatga tgctcaccag
     5461 cgttgcgctg ctgaagcgga atccccgccc cagcgaagcc gacatccgtc acgccatctc
     5521 gggcaatctg tgccgctgca ccggttacgt gaacatcgtc aaggccgtgc agtacgcggc
     5581 cgcaaagcgg taagagaggg agaaggggag aatgacgctc cgcacttcag ctgaagtctc
     5641 aggcatgggc cactcgatga agcggaagga agacccgcgc ttcatccggg ggaaaggcac
     5701 ctacgttgac gatatcacgc tgccgggcat ggtgtacctc gacatcgtcc ggagtccgct
     5761 ggctcacgcg aagatcgtct cgatcaacac cgagaatacg ctcaagattc ccggcgttct
     5821 cgccgtgatc accgggcaga cgctggctca gttcaatctt cactggatgc ccactctgat
     5881 gtcggacacg cagatggtgc tgccggtgga caaggtgatg taccaggcgc aggaagtggc
     5941 ggccgtggtc gcaaccgatc gttacgccgc cgctgatgga gttgccgccg ttgacgtgga
     6001 ttacgagccg ctgcctgtgg tagtggatcc gttcaaggcg ctcgagcccg gcgcaccggt
     6061 gctccggacc gacaagaagg acaagaagga caaccggatc ttccactggg aggccggcga
     6121 caaggaagcg accgacaagg ccttcgccgc ggccgacgtg gtagtcaaac aaagcctcta
     6181 catcccgcgg atccatgtcg cctcgatcga gacctgcggc tgcgtcgcgg acttcgatcg
     6241 ggtgcgcggc aagctcacgg tgtacatgac gacccaggcc ccgcatgcca ttcgcacggt
     6301 gttcgcgttg gtggcagggc acgtgggact ctccgaggaa aagatccgga tcatctcgcc
     6361 tgacatcggg ggcggattcg gcggtaaggt cccggtctat cccgggtacg tcattgcggt
     6421 agcggcgtcc gtggtgctgg gccgcccggt caagtggatc gaggaccgga tggagaacct
     6481 ccaggccgac tcttttgccc gggactatca cgtcaccgcc gagctggccg cgaagaaaga
     6541 cggcaggatg acggccctcc ggatcaagac ggtggctgat catggttatg cggacgctgc
     6601 ctgcaatcca tccaagtttc ccgcgggcct cttctcgatt tgcacgggct cctacgatct
     6661 caagaacgcc ttcgccgagg tcgatggggt ctacaccaac aagccgccgg gcgggattgc
     6721 ctatcgctgc tccttccgcg tcacggaggc ggtgcacacg atcgagcgca tcaccgacgt
     6781 gctggcccag cagctcggca tggacccggc ggagcttcgg ctcaagaact tcattccaaa
     6841 ggagcgcttc ccctacaagt ccgcgctggg ctgggaatac gacagcggcg attacaccac
     6901 tgctctcaag aaggccatgg agatcgtgga ctaccctgcg ctccggaaag agcaggctga
     6961 gaagcggaag cggggcgagc tgatggggat cgggatcgca agcttcaccg agatcgtcgg
     7021 cgccggtccc tcgaagcact tcgacatcct gggcctcaag atgttcgact cctgcgagat
     7081 ccgggtgcat cccaccggca aagcggtcgc gcggttcggg actaagtcgc agggacaggg
     7141 gcacgagacc acttacgcgc agatcgtcgc cgaggagctg ggtattccag cggcgcacgt
     7201 ggaggtggaa gaaggcgaca ccgacactgc gccatatggg cttggcacct acgccagccg
     7261 ctcgacgccg accgccggcg ccgccggcgc gatggcggcc cgcaagatcc gcgacaaggc
     7321 aaagaagatc gccgcacacc tgctcgaggt gagcgaaga
//
LOCUS       DASRJQ010000272         2295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2975304, whole genome shotgun sequence.
ACCESSION   DASRJQ010000272 DASRJQ010000000
VERSION     DASRJQ010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2975304"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..>2295
                     /locus_tag="VFB89_06115"
     CDS             209..>2295
                     /locus_tag="VFB89_06115"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HZM26911.1"
                     /translation="MSDDADGGMNRDAGRPPTDEAEVREAVERAHALLGGRGAAVDLE
                     AGLTRIGEMIRLARRLERHGGQDEVARTLIEDARAAAMDLAQRLDLDQFSERARAGTA
                     PIDDFAGLRRLIDQTIRVSRHLEYFGERTHARTLLAGARAAAINLAVDILRGAGSTPG
                     ETLALAKLLDRLDEFNFAWRILDKAREHRDFGREPGERIRIVQKRALYTYKDVTQPLG
                     KRLDEALTFLENEAELKITTDQETLGLAGAIHKRKWELTKNDRYLDVSYKYYAKGYDQ
                     KPWKDQGYTSINAAYLLDLLAQAHRDLPEQAEAHRHKADEIRAEIISVVPEMPKQPGQ
                     EWLEDMWFFYSTVGEAYFGRGDFEKAHLWLVTKPELRGVRPPDWEFETTARQLAELVR
                     TRNPDVDFASTPAWIAFRRFLSASPLGGGGDVSSEAVGRLAAGKVGLALSGGGFRASL
                     FHIGVLARLAELDVLRRVEVLSCVSGGSIVGAHYYLEVRKLLTEKPDHQIDKKDYIEI
                     VQRLEKEFLTGVQTNIRMSVVTEAKAVRRMLHRSYSRTVRLGELYEEQLYSRVAPING
                     EKVDAANAVRERYMHELAIHPKLAKGGQWTDFAPRNHNWRREAKAPILILNAAALNTG
                     HNWQFTASYMGEAPATIHREIDSVSRLRRKYYTDAPHSAAVPQKVRLGVAVAASSCVP
                     GLFEPLVFADMYET"
BASE COUNT          457 a          710 c          740 g          388 t
ORIGIN      
        1 gggtgaccga ggcagggttc gtagcgcact acccgaactt ctttgaaatc gttgggtgaa
       61 agtccgcccg ttccgctatc gagtggagtg ttccgcgaaa actaaaactc attttttgct
      121 tcatcctttc ctccagttgc cttaaaagag actcagcagc aactggttag attttcagtg
      181 aagcaatagc tggaaaagga gtattcccat gagcgatgac gccgacggcg gcatgaaccg
      241 cgacgccggt agaccgccga ccgacgaagc cgaggtgcgg gaagccgtcg agcgagcgca
      301 tgcgctcctc ggcgggcgtg gtgcggccgt cgacctcgag gcgggcctta ctcgcatcgg
      361 cgagatgatc cgcctcgcca ggcgcctgga gcggcacggc gggcaggacg aggtggcacg
      421 aacgctcatc gaagacgcgc gggcagccgc gatggatctg gcgcagcgtc ttgacctcga
      481 ccaattctcg gaacgcgcta gggctggaac ggccccgatc gatgacttcg ccgggctacg
      541 gcggctcatc gaccaaacga tacgcgtgtc cagacacctc gagtatttcg gggagcgaac
      601 tcacgcccgg acgctgttgg ccggcgcgcg ggccgctgcc atcaatctgg cggttgacat
      661 ccttcgcggc gccgggagca cgccaggcga gaccttggcc ctggctaagc tcctcgaccg
      721 ccttgacgag ttcaacttcg catggaggat tctcgacaag gcccgcgagc atcgtgactt
      781 cggcagagag ccaggcgagc ggatccggat cgtccagaag cgggcactct acacatacaa
      841 ggatgtcacc cagcccctcg gcaagcggct cgacgaggcg ctgacatttc tagagaacga
      901 ggcagagctg aagataacga cagaccagga gacgctcggt ctggcgggag ccatccacaa
      961 gcggaaatgg gagctcacca agaacgaccg ctacttggac gtttcgtaca agtattacgc
     1021 gaagggatac gaccagaagc cgtggaagga tcaggggtac acgagcatca acgccgccta
     1081 cctcctggac ttgctcgccc aggcgcatcg agacctgccg gagcaggccg aggcacaccg
     1141 ccataaggca gacgagatca gggcggagat catctccgtc gtccccgaga tgccgaagca
     1201 gcccggccag gagtggctgg aggacatgtg gttcttctac agtacggttg gcgaggccta
     1261 cttcggccgg ggcgatttcg agaaggccca cctgtggctg gtgaccaagc cggagctgcg
     1321 cggtgtgcgc ccgccggact gggagttcga gacgacggcg cgacagctcg ccgaattggt
     1381 gcggacccga aatcccgacg tcgatttcgc gtcgacgccg gcatggattg ccttcaggcg
     1441 attcctctcc gcaagcccgc tcggcggggg gggcgatgtg agcagcgagg ctgtggggcg
     1501 gctcgccgcc ggcaaggtag gcctggccct ttcgggcggc ggctttcgcg cttccctctt
     1561 tcacatcgga gttctcgctc gactcgccga actggacgtg ctccgccgcg tcgaggtcct
     1621 gtcgtgcgtc tccggagggt ccatcgtcgg cgcccactac tacctggagg tcagaaagct
     1681 ccttactgag aagcccgacc atcagattga caagaaggac tacatcgaga tcgtccagcg
     1741 tctcgagaag gagttcctca ccggtgtgca gaccaacatc cgcatgagcg tggtcaccga
     1801 ggcgaaggca gtccggcgga tgctccaccg gagctattcg cggacggtgc gactgggaga
     1861 gctctacgag gagcagctct actcgcgcgt cgcccccatc aacggcgaga aggtggatgc
     1921 ggccaacgcg gtgcgcgagc gttacatgca tgagctggcg atccacccca agctcgcgaa
     1981 gggtggtcaa tggaccgact ttgcgcccag gaatcacaac tggcggcgcg aggccaaagc
     2041 cccgatcctc attctaaacg ccgccgccct gaataccggt cacaactggc agtttacggc
     2101 cagctacatg ggcgaggcgc ccgccaccat tcaccgtgag atcgacagcg tctcgcggct
     2161 gcgtcgcaag tactacacag atgcccctca cagcgcggcc gttccccaga aggtgcggct
     2221 gggcgtcgcg gtggccgcct catcctgtgt acccggtttg ttcgagccct tggtgttcgc
     2281 ggacatgtac gagac
//
LOCUS       DASRJQ010000273         2481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3490869, whole genome shotgun sequence.
ACCESSION   DASRJQ010000273 DASRJQ010000000
VERSION     DASRJQ010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3490869"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VFB89_06120"
     CDS             <1..216
                     /locus_tag="VFB89_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26912.1"
                     /translation="NWLVQDPLFSGESGAIAYLPSQQVAIALTVTYEPAAFTSPASPK
                     NSADYLWRVIAAKLAPADAPMIPPNAQ"
     gene            375..824
                     /locus_tag="VFB89_06125"
     CDS             375..824
                     /locus_tag="VFB89_06125"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZM26913.1"
                     /translation="MRFDLTTRASPEQVLRALTDFTDSRLETWNRTLDPKTYELRESG
                     PTWAVARESSPRSPFWVVVRYDWSDAGTVRWTVVDSSYGGGGEGLVQIAPEVDGGSQV
                     HAEWTYTGARLMQRPPIFLINHGMSRFISRMWASALDRYAERGSAAS"
     gene            complement(1010..1480)
                     /locus_tag="VFB89_06130"
     CDS             complement(1010..1480)
                     /locus_tag="VFB89_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26914.1"
                     /translation="MTTPTSGYGVGVPKFGFKLDVPDPPAKVMAAMVDFSERRPEIWP
                     NLTARLYRVHELDARTADVTEGTALPGVDIWERVTYEWTDSVVRTVITDGRIFEPGGT
                     TEFRVEPHGAGSRISFDYHRRSKTVLGRCLGAMMQITGGAPIKHSWRQVYGKPS"
     gene            complement(1477..1638)
                     /locus_tag="VFB89_06135"
     CDS             complement(1477..1638)
                     /locus_tag="VFB89_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26915.1"
                     /translation="MTQAQETHCRIWLSQIKRLAALGQAARVLASLRAADGCDFAQEA
                     NRTNLGMRR"
     gene            complement(1875..1947)
                     /locus_tag="VFB89_06140"
     tRNA            complement(1875..1947)
                     /locus_tag="VFB89_06140"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1912..1914),aa:Arg,seq:cct)
BASE COUNT          440 a          801 c          802 g          438 t
ORIGIN      
        1 aactggctgg tgcaggaccc gctgttctcc ggcgagtccg gtgccatcgc ctacctgccg
       61 tcgcaacagg tggcgatcgc cctgacagtc acctacgagc ctgccgcctt caccagcccc
      121 gcctcgccga aaaactccgc cgactacctg tggcgcgtga tcgcagcgaa gctcgcaccc
      181 gcggacgccc cgatgatccc gcccaacgct cagtgagtta agcaccctcg gcggagatca
      241 tcgagctgcc cggcggaccg ctcgaagaag ttttcactcg gccaaagaaa cgcgcgccac
      301 aggtccctga acttatggct gtgccgagca ggccgcttct ggacagatgc caggtggcgt
      361 gctccgatga tcgaatgcga ttcgatctta cgacgagggc ctcgcccgag caggtactcc
      421 gggcgctgac agacttcacc gactcgcgct tggagacgtg gaatcgcacc ctggacccga
      481 agacatacga gctgcgcgag agcggtccga cctgggctgt tgcgcgggag agcagtcctc
      541 gatcaccgtt ctgggtcgtc gttcgctacg actggtccga cgcggggact gttcgatgga
      601 ccgtcgtgga cagcagctac gggggaggtg gcgaggggct ggtccagatc gcaccggaag
      661 tcgacggggg cagccaggtg cacgcggagt ggacctacac cggggcgcgc ctgatgcagc
      721 ggccacccat attcctgatc aatcacggga tgtccagatt catctcgcgc atgtgggcct
      781 ccgctctcga ccggtatgcg gaacggggct cagcggcgtc ctagagcgtg ggtcgaacca
      841 ttggcccgta aaccgggtgg acctgagcgg accgccggtt caaggcgcgc gcgctggtcg
      901 tcggctggcg gtcgccgacg cctggtcaga gcagtttcgg ccgcgggcgt acttggttga
      961 gcagggcaac tgggatggcg ccgacacgag gcctcgacat tcggaccccc taactcggct
     1021 tgccgtagac ctgtcgccaa gagtgcttga ttggcgcccc accagtgatc tgcatcatcg
     1081 cgccgaggca ccgtcccaac accgtcttgg atcgccggtg gtaatcgaag ctgattctgc
     1141 ttccggcgcc gtgcggctcg acacggaact cggttgtacc gcccggctcg aagatccttc
     1201 cgtcggtaat cacggtgcgc acgaccgagt cagtccactc gtaggtgacg cgctcccata
     1261 tgtccacgcc gggcagcgca gtgccctccg tcacgtcagc ggttcgggca tcgagctcat
     1321 gcactcggta caggcgcgcg gtgaggttcg gccagatctc cggccgacgc tccgagaaat
     1381 cgaccatcgc ggccattacc ttcgcgggcg ggtcgggtac atcgagcttg aagccgaact
     1441 tgggcacgcc gacaccgtat ccggaggtcg gagtcgtcaa cggcgcatgc cgagattggt
     1501 gcggttggct tcttgagcga aatcgcatcc gtccgcggcg cgcagcgaag cgaggacacg
     1561 cgccgcctgg ccgagcgcag cgaggcgctt gatctgggag agccagattc ggcagtgcgt
     1621 ctcctgcgct tgtgtcaagg catgcgtcaa gacatctgcg gtataccgct ctagttcgct
     1681 gctcgacgct gttggcagtc ggggcgcggt cggcgcttgg tgttcagcgg ctggggtcag
     1741 gcggcccgcg ctgactggtt aactacctga tggcggtgat ggtgactgga tgtgcggcct
     1801 gagccctcgg cggagatcat cgaactgccc cagcggatcg cccgaagaag ttctgactag
     1861 gccaaacgtg tcagcgcccc cagcaggatt cgaacctgcg acacgaggtt taggaaacct
     1921 ctgctctatc ccctgagcta tgggggctga ctaaacaccg cgggtcgtct tccctcgtgc
     1981 cattagcttg ccaaactccg tgtacagata gcggttgcta acggccactc ggagcagcgt
     2041 agtggcacgc tcttgccgca gctgagatcg gtccgatccc tggccgttag tcctattccg
     2101 gcgctcaaat ccaccccgac ggagtcccct gaggaagcct tgattccgga tggcggaaac
     2161 taatgtccga aggagccctt tccaccgtca tgcgcccacg cgtacgctct aaccacgaac
     2221 ccaacctccg ccgcagccgg tggctgccga agtgacacgc cgcgccatgg gctgccgacg
     2281 gaccgtcaga ctggacagag ggcgagccga tgatcacccc ttatgcaacg gtcaacggcc
     2341 gaacggtcgc cttcggcaac ctgccgacac acacctcggc gctggactgg atccgcgacc
     2401 tcggctttac aggtgccaag gagggttgtg ccgaagggga gtgcggggcg tgcgcgatcc
     2461 tggtggcgcg accgagcacc a
//
LOCUS       DASRJQ010000274         2946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1032770, whole genome shotgun sequence.
ACCESSION   DASRJQ010000274 DASRJQ010000000
VERSION     DASRJQ010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032770"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VFB89_06145"
     CDS             <1..185
                     /locus_tag="VFB89_06145"
                     /inference="COORDINATES: protein motif:HMM:NF037182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26916.1"
                     /translation="LEILGPAPCPLARIKERWRWHVLLKGPSRAVGRLVRYGARRLTR
                     AGDIRVVVDRDPASVL"
     gene            920..1633
                     /locus_tag="VFB89_06150"
     CDS             920..1633
                     /locus_tag="VFB89_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26917.1"
                     /translation="MIHPAAWVGIRGASTELSVSGWGNWESGNAGSADLTQRGIGRRG
                     FGEADLDVAVSREFGPTAVAAGWTRYTFHGDAAGAGQTSSDNTSELFVHFAWHGSTVT
                     PSVLAACDVDRVRGCYFEVGAPLPIFATSEPRPTLIFSIEPTAGWSQGEGPNSDDPTQ
                     LSNFAGNGLTHVDLPFLAQAQFSGGTLEPAFSLAAHLQWCADGATRTTNATGSTTRVK
                     FWLEVGATIAAGLPRKQRR"
     gene            1630..>2946
                     /locus_tag="VFB89_06155"
     CDS             1630..>2946
                     /locus_tag="VFB89_06155"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HZM26918.1"
                     /translation="MTRVGIINLTLLVAACAKASPPVELPALASAASPQPVAVRGLVA
                     GIGRADITPPPGLGLGGRGPEGRVAAGYRHRLYVRSLFLEDGRGERVVFVIADLPFVS
                     VLLHRRVAELLQPRTGIGADRLILAATHTHAGPANIEDWRLHNELASSVSGYDSAFAG
                     RVAAAIAAAAAEAVDSARPARIRFGATAVWGLTRNRSLAAYQRNPPHSASMSPPPALD
                     SASAAVDPTLTMLRVDLRAPGDSVFRPAGAFSIFAIHGTGDPAENDLYDGDIHAVVER
                     ALERHIDSLAGLPASARRAVHLFADGAEGDVSPAMPPESWCAPPRLTPERLHGGQRAS
                     RSWEWLPPGSREACVDAARLGVAFIGDSLGRSVVSLFDALEPGPEASELLIARATRTL
                     PLVGPRAPPELCAEPTPGASVMGGAEDGPTRYRGWRWLGLIPSSWEE"
BASE COUNT          470 a          966 c          948 g          562 t
ORIGIN      
        1 cactcgagat cctgggcccg gctccgtgtc ctttggcgcg aatcaaggaa cggtggcgat
       61 ggcacgttct actcaaaggt ccatcacgcg ccgtaggccg gctcgtgcgc tatggcgcgc
      121 ggcggctcac gcgggcgggc gacattcgcg tggtggtcga ccgggacccg gcctcggtgc
      181 tttgaccggc acctcttgcc gcgagttgcc tcggcagttg gtttcctaga cagctccgca
      241 atcgcaattg gccgctgcca ctgcgaccgt gatcgcgagt gctgtgcccg cgatgacaac
      301 gccgagcaaa cgctcttcag ggggctgccc tgacgggtgg ctatggtcgt aatgtctgcc
      361 agggggacgc catgggttgc accctgggcg acgccgatga ggctgtcgcc cacgatggtc
      421 ggtttcgcta caaccaatgg ggcctggccc acccggctga ttcgtacttc gtgaggcgat
      481 ttctccgtca cgagttgcgc gggtgggact tgctctacac gccaactcga gcaggcggcc
      541 atctggatga cgaaaagtgc gcaggccatt cgtggtaccc aggcaagccc gatgatttct
      601 gtaggaccct tcattcgaga tcttccgccc atgaggttca caccaccggt gcgctctgaa
      661 cctacattca ccgagacgga agtacgctgc tctcctccgg taaagcagca atcaagcggc
      721 tatagccgtg ctcgcggcta ctgaggcgtc gttgccgcag cacgcggccg gcgtaccttg
      781 ggcgatccgc gcggcgctct tggtcgtgct tgcaacgctg gaacctggtt ccggttgtgc
      841 ggcgcagatc gcgagcggcg cctctaccag tattgcaacg cgatatctgt ggaggggaat
      901 aacacgcgtt agtgattggg tgatacaccc ggccgcgtgg gttggaatcc ggggtgcctc
      961 gaccgagctg tcggtatccg gttgggggaa ctgggaatcg gggaacgccg ggtcagcaga
     1021 ccttacccaa cgcggcatcg gtcgccgcgg cttcggcgag gcagatctgg acgtcgcagt
     1081 gtcccgggag ttcggcccta ctgccgtggc cgcgggctgg actcgctata ccttccacgg
     1141 cgatgccgca ggcgccggac aaacgtcgtc ggataacacg agcgagctgt ttgtgcattt
     1201 cgcgtggcac ggctcgaccg tgaccccttc tgtcctcgcg gcctgtgacg tagaccgcgt
     1261 gcgcggttgc tacttcgagg ttggtgcccc actcccgatc ttcgctacct cagaaccccg
     1321 acccacactg atcttcagca tcgaacccac cgcaggctgg agccagggcg aaggcccaaa
     1381 cagcgacgac ccaacccagc tctcgaattt cgcgggcaac ggactcaccc acgttgatct
     1441 gcccttcctg gcccaggcgc aattctccgg gggcacgctc gaacccgcct tctcacttgc
     1501 ggcccatctc caatggtgtg ctgatggcgc gacgcgcacg accaatgcga ccggttccac
     1561 tactcgagtc aagttctggc tcgaggtcgg cgcaacaatc gcggctgggc tgccgcgcaa
     1621 gcaacgccga tgacgcgcgt cggcattatc aacctcaccc ttctggtcgc tgcctgcgcc
     1681 aaggccagcc caccggtcga actgccggcg ctcgcatctg cggcttcgcc gcaaccagtt
     1741 gcggtccgcg gcctcgtcgc gggaatcgga cgagccgaca tcacgcctcc ccctggcctc
     1801 ggcctcggcg gccgcggccc cgaaggtagg gtggcagccg gctatcggca tcgactatac
     1861 gtgcgatcgc tgttcctgga ggatggccgc ggcgagcggg tcgtgttcgt gattgctgat
     1921 ttgccgttcg tgtccgtgtt gctgcatcgc cgcgtggcgg agcttctgca gccaagaacc
     1981 ggaattgggg cagatcggct aatcctcgcc gcgacccaca cgcacgccgg gcccgccaac
     2041 atcgaggatt ggcgcttgca caacgaactg gcgtcgtcgg tgagtgggta cgattctgca
     2101 tttgccggaa gggtggcggc tgccattgca gcggctgccg ccgaagcggt cgacagcgcg
     2161 cgtccagcgc ggatccgttt tggcgcgact gctgtatggg gactgactcg gaaccgaagc
     2221 ctcgcggcct accagcggaa tcctcctcat tcagcatcca tgtccccacc acctgcgctc
     2281 gattccgcat ccgcggctgt tgatcctacg ctgacgatgt tgagggtcga tctgcgtgcg
     2341 cctggggact cagtcttccg gccggcaggc gctttttcaa ttttcgcgat ccacgggact
     2401 ggcgacccgg cggagaacga cctgtacgat ggcgacatcc atgccgtggt cgagcgcgcc
     2461 ctcgaacggc atatcgattc gctagctgga cttccggcca gtgctcgtcg ggcggttcat
     2521 ctttttgccg acggggcgga gggcgacgtc tcgcctgcaa tgcccccaga aagctggtgc
     2581 gctcctcctc gacttacacc cgagcggctc catggtggac aacgcgcatc gcgcagctgg
     2641 gagtggctac caccagggtc gcgcgaggcg tgcgtcgatg ctgcccgctt gggtgtcgcc
     2701 ttcatcggcg acagcctcgg ccggagcgtg gtaagcctgt tcgacgcgct cgagcccggg
     2761 cctgaagcct ctgaactgct gattgctcgc gccacccgaa cgttgccgct ggttggtccg
     2821 agggcaccac cggaactctg cgccgagcca acaccaggag cctccgtcat gggaggcgcc
     2881 gaagacgggc cgacccgcta tcggggttgg cgctggctcg gcttgatccc ttcttcctgg
     2941 gaggag
//
LOCUS       DASRJQ010000275         2865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2866669, whole genome shotgun sequence.
ACCESSION   DASRJQ010000275 DASRJQ010000000
VERSION     DASRJQ010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2866669"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2301)
                     /locus_tag="VFB89_06160"
     CDS             complement(<1..2301)
                     /locus_tag="VFB89_06160"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZM26919.1"
                     /translation="MYFWLGIGLAKAAAGFLAWLFTLWDIEREWAVLPPAIHVALMAA
                     FAGTGIALVAGSRPGPARSLGVVFCLFSSIFADPLVERLTPADMGAWLRRPVLALVPV
                     ALAPAYFWRFAWEFPRFQPALLPAWAPGLFERVTFAAGGAMFCAAALFSLGDVRNGLS
                     GVSWQDLIWTGIVLLALPTVALLVAKARTALPDERRRVTLFISGLVLGMAPLHVDILL
                     SAVLPPYREFFQAPARTRVVAGILAAATLLIPAVTAYAVVVDRVLETRYMVRLAIQYA
                     LARYTVLGLALVPVAFLARHLYIHRLEPLVDILTRAPLPVWLALLGLTLYLSRVRRSL
                     LHAIDRRYFREQHDARSVLASLTDSTRRVASLEQLARLVASEIDNALHVDKVALLVRN
                     ENAFEDPLGATPSLAADGTLATLAGGSSSAFDLEATTSQSPLRRLPMQDIEWIDSADA
                     TVLLPLIDSGGSLLGLLVLGGKRSEAKYSSEDRLLLQAVASAVLLTLEQRLHQRTPLP
                     FGQGAALDAADSEPAQQCDRCERIFKSTLTTCEGCLIALRPASVPSLLAGKFRVLSRI
                     GSGGMGIVYMGEDVDLNRPVAIKTLPRLSEAAVRRLRREARALATIHHPNLEGIYGVE
                     SWRGTPMLVLEYLSGGTLASRLRGGPLEVQTVIETGAAMADALHSLHRAGVLHRDIKP
                     SNIGFTGDDTPKLLDFGLAVVAAPDFAKPSGDLSEHGESITRTRAMFASAPDQSSGIL
                     AGTPLYMSPEAIAGGKPDVGFDLWGLA"
     gene            complement(2317..2652)
                     /locus_tag="VFB89_06165"
     CDS             complement(2317..2652)
                     /locus_tag="VFB89_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26920.1"
                     /translation="MPENATVEGKILQLAMAFGQGAGVMLATEQALLVALGAYRESVN
                     TRARDWNDYSLQAIEYGRVLGSLAAHHALANSRCVIDEDDVRYGLDAVTDNARTPLGF
                     CRLTLRERQ"
BASE COUNT          585 a          923 c          918 g          439 t
ORIGIN      
        1 ggcgagcccc cagagatcga acccgacgtc aggcttcccg ccggcaatgg cctcaggcga
       61 catgtacagt ggcgttccgg cgaggatacc gctcgactga tcaggcgctg acgcgaacat
      121 cgcacgagtt cgtgtgatcg attcgccgtg ctcagaaagg tcaccggacg gcttcgcgaa
      181 gtcaggtgcc gcgacgacgg ccaacccgaa gtccagcagc ttgggcgtgt cgtcgccggt
      241 gaacccgata ttactgggtt tgatgtcgcg gtgaaggacg cccgcgcgat gcaaactgtg
      301 aagtgcatcg gccattgccg ccccggtctc gatcacggtc tggacctcca gcgggccgcc
      361 tcgcagacga gaggccaggg tcccgccgga caagtactcg aggacaagca tcggagtgcc
      421 gcgccacgac tctaccccgt agatcccctc caggttcggg tggtggatcg tcgccagggc
      481 ccgcgcctca cgccgaagcc gccgtacggc cgcctcggat agccggggca gcgtcttgat
      541 ggccaccggg cggttcaaat ccacgtcctc ccccatatag acgatgccca tgccgccgga
      601 accaatgcgg ctgaggaccc ggaacttacc agccagcagg ctcggaaccg acgccgggcg
      661 aagggcgata agacatccct cgcacgtggt gagcgtggat ttgaagatgc gctcgcaacg
      721 gtcgcactgt tgggcaggct cgctatcagc ggcatcgaga gccgcgccct gcccgaaggg
      781 cagcggggtt cgctggtgga ggcgctgttc cagcgtcagg agaacggcac tggcaaccgc
      841 ctgcaacagg agacggtctt ccgacgagta cttggcttcg ctacgctttc cgccgagcac
      901 gagcaggccc agcagcgacc cgccggaatc aatcagaggc agcagcacgg tggcgtcggc
      961 cgaatcgatc cactcgatgt cttgcatcgg gagtcgcctc aagggcgatt gactcgtggt
     1021 ggcctcgaga tcgaacgccg acgatgagcc ccctgcgaga gtcgcgagtg ttccgtctgc
     1081 tgcgagggag ggcgtcgcgc caagaggatc ttcgaacgca ttctcattgc gaaccagcag
     1141 agcgaccttg tcgacatgaa gcgcgttgtc tatctcgctc gcaaccagcc tggccagctg
     1201 ctcgagcgag gcgactcgtc gcgtactatc cgtgaggctc gccagaacac tccgggcatc
     1261 atgctgctca cgaaaatagc gacgatcgat ggcgtgcagc agcgagcgcc gtacacgtga
     1321 caggtacaac gtgagtccca gcagggccag ccataccggc aaaggcgccc gagtgaggat
     1381 atccaccagc ggttccaggc ggtggatgta cagatgtctg gcaagaaacg cgaccggtac
     1441 gagtgccaag ccgaggaccg tgtagcgggc gagcgcgtac tgaatggcca atcggaccat
     1501 gtatcgcgtt tcgagcacac gatcgacgac gacggcgtac gcggtcacgg cgggtatcaa
     1561 cagggtcgcc gccgccagga tcccggcgac gacacgggta cgggcgggcg cctgaaagaa
     1621 ctcccgatac ggcggcaaga ctgcagacag caggatgtcg acgtggaggg gtgccatccc
     1681 gagcacgagc ccgctgataa agagcgtgac gcgccggcgt tcgtctggaa gcgccgtgcg
     1741 cgctttggca accaggagcg ccaccgtagg cagcgccagc aagacaatgc ctgtccagat
     1801 caggtcctgc caggacacgc cgctcagtcc atttcgcacg tcgccgagcg aaaagagcgc
     1861 ggccgcgcag aacatggcgc caccggccgc gaaggttacg cgctcgaaaa gcccaggcgc
     1921 ccacgcgggc agcagggcgg gctggaagcg tggaaactcc cacgcgaacc gccagaaata
     1981 ggctggtgcc aatgcaaccg gaactaaagc gagtaccggg cgtcgcagcc acgctcccat
     2041 gtcggctggc gtcaggcgct caacgagggg atcggcaaaa atgctcgaaa acagacagaa
     2101 aaccacgccg agcgaccggg ccggaccggg ccgggaaccc gccacgaggg cgatcccggt
     2161 acccgcaaac gctgccatca gcgctacatg gatcgccggg ggaagcacgg cccactctcg
     2221 ctcgatgtcc cacagggtga acagccaggc gagaaagccg gctgccgcct tcgccagccc
     2281 gatcccaagc caaaagtaca tggacggccc cccggtttac tgacgctctc gaagcgtcag
     2341 cctgcagaag ccaaggggcg tgcgagcatt gtccgtcacc gcatccagcc cgtaccgaac
     2401 atcgtcctca tcaatcacgc agcgtgagtt tgccagcgca tggtgcgccg cgaggctgcc
     2461 cagcacccga ccgtactcga ttgcctgaag cgaatagtcg ttccagtcgc gcgcgcgggt
     2521 gttcacactc tctcggtagg cgcccaacgc aacgagcaaa gcctgttctg ttgccagcat
     2581 gacgccggcc ccctgaccga acgccatggc cagctgcaga attttcccct cgacggtcgc
     2641 gttttccggc atggttggct ccttcccgcc actcgggtct agtctggtcg cccagtgtga
     2701 tgattttccg gccgagatcg gctcggaggt gcagattcta gcgcaacgac tgagcggacg
     2761 ggccgcgttc gaatccgtgg aacggccggc acggtaacat gctgcacgcg actcttttga
     2821 cgaggtctgt cgtgctgaca tatgatgtgc ccacatatgg gcatg
//
LOCUS       DASRJQ010000276         7009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3858531, whole genome shotgun sequence.
ACCESSION   DASRJQ010000276 DASRJQ010000000
VERSION     DASRJQ010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7009
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3858531"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1325
                     /locus_tag="VFB89_06170"
     CDS             <1..1325
                     /locus_tag="VFB89_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26921.1"
                     /translation="RPEAAAAAARECRSVQWWCTALSAFVLHAQGDDSAADTLFAEAL
                     AAMPRAEKCVWMDLTPLLGDQAGSYAKLPCEAREGGNLRWWWLGRPLYLRPGNDLRVE
                     HFSRLVMARIRSGESLERPLDVGGNYSELIIRYGWPVIWLRAPRRPGDLEPGRLVTRE
                     RIPAWSFFPSAKQPPVWQLDSASAASIYAPIWATTFSTIDHLQIARFRRSDSVVTIAG
                     FDLSSDTVLAGLTPTVALAVGTDAASPVVLSPVLKKLYGAVAVQSADPPTMVSLEAIR
                     EEARWAGRFRSVTTDAATWFTSPLSDILLVRAEAASTGALDALASIALPGTVLPRGQP
                     VGIYWEWYERPPAGAVVAIEARVAKIGGKGRIDPLGRSACAPPGKAAMAVQWREPVTS
                     QRMGVGRAVALDLTRLESGRYLIAISVSGEGASAPPRCTSREIELAGR"
     gene            1397..2074
                     /gene="radC"
                     /locus_tag="VFB89_06175"
     CDS             1397..2074
                     /gene="radC"
                     /locus_tag="VFB89_06175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HZM26922.1"
                     /translation="MTAPVGVPAERPRERLWSVGPAALTGAELLAILLGTGGVGEPVG
                     SIAVRLLEMADGSLRRLAARPAAELLRLPGVGPTKAARLLAAFELAARVSREGRATLP
                     RIREPDDVVRLFQARLRDLPVEEFHLLALDSQSQVLRDVLVTRGLLNSSLVHPREVFR
                     PAIAEAAAGIIVVHNHPSGDPTPSAEDRAVTRQLVAAGQLLDLPVYDHVILAGDRFLS
                     FAAEGLL"
     gene            2271..4445
                     /locus_tag="VFB89_06180"
     CDS             2271..4445
                     /locus_tag="VFB89_06180"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HZM26923.1"
                     /translation="MTVSPPLLANATPEFRAVLERHADRLDLELIDRALKYSSSAHRG
                     QKRMSGEDFISHSIAVALILAEQLLDTTSIAAALLHDVVEDSEVRTEDMAREFGPEIT
                     GIVDGLTKISHLTFRSSAEEQVENYRKLLLSIARDARVIIIKLADRLHNMRTLEHLPR
                     DRRQRIALETREIYAPLAHRFGMAGVKAELEDLAFKFLEPEEYRDLVTQIAAKRAVRE
                     QMILKLRTPLEYELRRAGIEGVEVTGRPKHLWSIYQKMKKRGKSFDEIYDLMAVRVIV
                     KTVPECYHVLGIIHHNWTPIQERIKDYIASPKSNAYQSLHTTIFGPGGQLFEVQIRTH
                     EMHQTAEYGIAAHWLYKKDGTRDELDQHLGWFRQLLELQQDTHSPEEFLEFLKIDLYQ
                     DEIFVFTPKGDVKRLPKGSTPIDFAFHVHTDVGLRTQGARINGRIAPLHRELKNGETV
                     EIIAGPNAKPSRDWLNHVRTARARHKIKQWIKHEEEKVSLALGKEILAREVRRRRLES
                     PDDAQLERAARTLSLADGPGLEIAVGRGDVAIGQVIRALYPDLAPDAIQEPKPTVFGR
                     VIDRIRLGRGIKIQGVDGLMVRYAQCCQPVPGDQVIGYVTQGRGISIHRADCPNLLTL
                     AADERRVEIDWKEVQGEAFRVRLAVNADDRRGLYADLMEAISQTGTNIKGADLHTKDG
                     SVFGTIFVEVDNLPHLAKVLKGIRRVKGVTNVERREATPTGN"
     gene            4552..5148
                     /locus_tag="VFB89_06185"
     CDS             4552..5148
                     /locus_tag="VFB89_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26924.1"
                     /translation="MPRSQTPLHSALVALVAVLGVGGCQTQSRSNGIETGSLGDRSYS
                     DTGVVSDTGKTSDSAKPAEPGKLRVTMVMIGKGLGPDKRISEPTLRFAPTDTVFVSIG
                     TEGTPQSSTLMAKWRFPTGKVRDSSSTSIQPKGPQYTELHAGPPKGGWPVGPFQVTVY
                     VDGDSVDAKTFAVDKPSDTGKAVGGDTAAKTGDSTSTQ"
     gene            5226..5696
                     /locus_tag="VFB89_06190"
     CDS             5226..5696
                     /locus_tag="VFB89_06190"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HZM26925.1"
                     /translation="MTAHWILKTEPSTYSFDRLLEERKTVWDGVSNPVALKHLREMTV
                     GDQVMIYHTGDVKAVVGLARITRAAYPDPKDSKLVVVEIEAGERLPHPVTLAAIKADP
                     AFADLALVRMGRLSVVPVKPEQWKQITQMAEEQGEGERGKGEKGKGERGKGRGG"
     gene            5803..>7009
                     /locus_tag="VFB89_06195"
     CDS             5803..>7009
                     /locus_tag="VFB89_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HZM26926.1"
                     /translation="MSPRFEVVAPFEPAGDQPKAIDELTGGLRRGERYQTLLGVTGSG
                     KTMTLAHAIAAHQKPTLVLSHNKTLAAQLYGELRQFLPANAVEYFVSYYDYYQPEAYV
                     PATDLYIEKDASINEDIEMLRLRATSSLMEREDVVIVATVSAIYGLGDPLEYRQLMVS
                     VKRGERRGRDSILAELVRIQYGRNDASFEPGTFRVRGDSLEIYPAYAEQAVRIELWGD
                     EVERISKINPLTGQTIAQLDQMAIYPAKHFVTQRPTIERAVKLIRAELADRLQLFRSQ
                     GKLLEAQRLESRTNFDIEMLLEIGTCAGIENYSRPLSGRQEGERPAVLLDYFPPDFLV
                     VVDESHVTLPQLGGMYNGDRARKLTLVEYGFRLPSALDNRPLQFDEFMSLVPQMVNVS
                     ATPGDFELRL"
BASE COUNT         1262 a         2239 c         2260 g         1248 t
ORIGIN      
        1 gccgccccga ggcggccgcg gccgctgccc gggagtgccg atcggtccaa tggtggtgca
       61 cggcactctc ggctttcgtg cttcatgcac agggagacga ttccgcggcc gacaccctct
      121 tcgctgaggc gctcgccgca atgcccaggg ccgagaagtg cgtctggatg gacctaacac
      181 cgctgctcgg cgaccaggcg ggatcgtacg ccaagctgcc ctgcgaggcg cgcgagggtg
      241 gaaacctgcg ctggtggtgg ctggggcgcc ctctctatct ccggccgggc aacgatctgc
      301 gggtggagca cttctcccgg ctggtgatgg ctcgcatccg cagcggtgaa agtttggagc
      361 ggcccctcga tgtcggggga aactactcgg aattgattat ccgctacggt tggccggtaa
      421 tctggcttcg agctccgcgt cggcccggtg acctcgagcc gggaaggttg gttacccgag
      481 agcgaatccc tgcctggtcg ttcttccctt ccgccaagca gccgcccgtc tggcaactcg
      541 actcggcgag cgccgcctcg atctacgcgc ccatctgggc aaccacgttc tcgaccatcg
      601 atcacctgca gatcgcccgc ttccgccgca gcgactcggt ggtcacgatc gccgggttcg
      661 acctcagcag cgacacggtg ctcgcgggac tgaccccgac ggtcgccctc gcggttggca
      721 ccgatgctgc cagtccggtt gtgttgagcc cggtgctgaa aaagttgtac ggtgccgtcg
      781 ccgtgcagtc agccgatccg ccgaccatgg tgagcctgga agccatccgg gaagaggcac
      841 gttgggccgg ccgctttcgc agcgtcacga ctgacgcggc gacgtggttc accagcccct
      901 tgtccgacat cttgctggtg cgagccgaag ctgcgagcac cggcgcactc gacgcgctgg
      961 cgtcgatcgc tctccccggc accgtcttgc cccgcgggca gccggtcggg atctattggg
     1021 aatggtacga gcggcccccg gccggagcgg tggtcgcaat cgaggcccgg gtcgccaaga
     1081 tcggcggaaa gggacgcatc gatccgttgg ggcgctccgc ctgcgcacct ccgggcaagg
     1141 ccgccatggc cgtccagtgg cgcgaacccg tcaccagcca gcgaatgggc gtaggtcgag
     1201 cagtcgcgct cgatctcacc cggctcgagt cgggtcgcta tctgatcgcc atctccgtca
     1261 gcggggaagg ggcgtcggct cctccgcggt gcacgtctcg cgagattgaa ctggctgggc
     1321 gctgaccgca cgcattcgcg ccgcggcgcg cagaagcggc actcgcttct tcccccgttc
     1381 aagcgaagct ccacaagtga cagcgcccgt cggagtacct gccgagcgtc ctcgcgagcg
     1441 tctgtggagc gtgggcccgg ccgcgcttac cggcgccgag ctgcttgcca ttctgctggg
     1501 aacgggcggc gtcggggagc ccgttggctc gattgctgtc cgcctcctcg agatggcaga
     1561 cgggtcgttg cgtcgactag cggcacgccc ggcggcagag ctgctccgtc tgcctggtgt
     1621 cgggcccacc aaggccgcgc gactgctcgc cgctttcgag ctggcggcgc gggtgagccg
     1681 cgagggtcga gcaacgctgc cgcgtatacg cgagccggat gatgtcgttc gcctgttcca
     1741 ggcccggctg cgtgacctgc cggtcgagga gttccatctt ctcgcgctgg acagtcagag
     1801 ccaggtgctc cgtgatgtcc tggtcacgcg agggttgctc aacagctcac tggtgcaccc
     1861 acgcgaagtg ttccgccccg cgatcgcaga ggcggccgcg gggatcatcg tcgtgcacaa
     1921 ccaccccagc ggagatccca ctccctcggc ggaggaccgg gccgttaccc gccaactggt
     1981 cgccgccggc cagctgctcg acctgccggt atacgaccat gtaattctgg caggtgaccg
     2041 gtttctgagc tttgccgcgg aaggcctgct ctagccgata ctatcccctg actcatcctc
     2101 atccctgctc gactatcacg ctaggtcgcc ggtctagatt gatgcgatat cgtggcttac
     2161 ctcttcccgg cgcgaatctc cgcgagctgg gcaaaggtgc cgttacggtt gattattccg
     2221 gccgacggcc gcattctctt tgtgatgtgc acgacgctac ggggccgtcc atgacggtct
     2281 ctcctccgtt attggccaat gccacgcccg agttccgggc ggtgctcgag cgccacgccg
     2341 atcggctcga tctcgagctc atcgatcgag cgctcaagta cagctcgtcc gcgcatcgcg
     2401 gccagaagcg gatgtccggc gaggatttca tctcgcactc gatcgctgtc gctctgatcc
     2461 tggccgagca gctgctcgac acgacgtcga tcgctgcggc cctgttgcac gatgtagtgg
     2521 aagactcgga agtccgaacc gaggacatgg ctcgcgagtt cggacctgag atcaccggca
     2581 ttgtcgacgg tctcaccaag atttcacacc tcaccttccg ctcctcggcg gaagagcagg
     2641 tcgagaacta ccgcaagctg ctactgtcga tcgcccggga tgcacgggtg atcattatca
     2701 agcttgccga ccgactgcac aacatgcgca cgctggagca tctgccgcgc gatcggcgcc
     2761 agcgcatcgc gctcgagacc cgcgagatct acgcgccgct ggcacatcgc ttcggcatgg
     2821 ccggcgtcaa ggcggagctc gaggatttgg cgttcaagtt tctcgaacct gaggaatacc
     2881 gcgacctggt gactcagatc gccgccaagc gcgccgtgcg ggagcagatg atcctcaagc
     2941 tcaggacgcc gctcgagtac gagctcaggc gcgccggcat cgagggggta gaggtcaccg
     3001 ggcggcccaa gcatctctgg tcgatttacc agaagatgaa aaagcggggc aagagctttg
     3061 acgagatcta cgatctcatg gcagtgcggg tcatcgtcaa gacggtgcct gagtgctacc
     3121 acgttctggg aatcatccac cacaactgga ccccgatcca ggagcggatc aaggactaca
     3181 tcgcgagccc caagtcgaac gcgtaccagt cactgcatac caccatcttc ggcccgggcg
     3241 ggcagctgtt cgaggtgcag atccgcaccc atgagatgca ccagaccgcg gagtacggca
     3301 tcgcggcgca ctggctgtac aagaaagacg ggactcggga cgagctggac cagcatctcg
     3361 gctggttccg tcagctcctc gagctgcaac aggacaccca cagccctgag gagtttctcg
     3421 agtttctcaa gatcgacctg tatcaggacg agatcttcgt gttcaccccc aagggcgacg
     3481 tcaaacggct gcccaaggga tccacgccga tcgacttcgc cttccacgtt cacaccgatg
     3541 tcgggctccg cacccagggc gcccggatca acggacggat cgcgccgctc catcgcgagc
     3601 tcaagaacgg agaaaccgtc gagatcatcg ctgggcccaa cgcgaagccg agccgggact
     3661 ggctcaacca cgttcgcacg gcgagggcca ggcacaagat caagcagtgg atcaagcacg
     3721 aagaagagaa ggtgagcctg gcgctgggca aggaaatcct ggcccgcgag gtgcgccgcc
     3781 gccggctcga gagcccggac gatgcccagc tggagcgggc cgctcggacg ttgtccctgg
     3841 ccgacggtcc gggtctcgag atagcggttg gccgcggaga cgtcgcgatc ggccaggtga
     3901 ttcgagcgct gtaccccgat cttgcgccgg acgccatcca ggagccaaag cccaccgtat
     3961 tcggccgcgt gatcgatcgg attcgcctgg gccgcggcat caagatccag ggcgtggacg
     4021 ggttgatggt gcggtacgcg caatgctgtc agccagtgcc cggagatcag gtgatcggct
     4081 acgtcactca gggacggggg atttccatcc atcgcgcgga ttgccccaac ctgctcacgc
     4141 tggcggcaga tgagcggcgg gttgagatcg attggaagga agtgcagggc gaggcattcc
     4201 gagtgcgact ggcggtcaat gccgatgatc gccgcggctt gtatgccgat ctcatggagg
     4261 ccatcagtca gaccggcacc aacatcaagg gtgcggatct tcacacgaag gatggttctg
     4321 tcttcggtac catcttcgtc gaggttgata accttcccca tcttgccaag gtcctgaagg
     4381 gcattcgccg tgtgaaaggc gtcaccaacg tcgaacggcg agaggcgacg cctaccggca
     4441 attagtcagg tgagcgtggt cacatcgaag agcccctcca tttttcatcg tgtcgcttcc
     4501 acgagcccgg tgcatcgggc cgcaagccac gagagcatga ctggaggagc catgcccagg
     4561 tcgcaaaccc cgttgcattc cgccctcgtc gccctcgtcg ccgtgctcgg cgtcggcggc
     4621 tgccagacac agtcccgctc gaatggaatc gagacgggct cactaggtga taggtcgtac
     4681 tccgacaccg gagtcgtttc agatacgggc aagacgtccg attcggcgaa gccggctgaa
     4741 cccggcaagt tgcgtgtgac gatggtcatg attggtaagg ggctcggccc ggacaagcgg
     4801 atcagcgagc cgacgttgcg ctttgcgccg accgataccg ttttcgtttc aatcggcacg
     4861 gagggaacgc cgcagagttc cacgttgatg gcgaagtgga gattccctac cggtaaggtg
     4921 cgtgactcca gttcgacgag tatccagccg aaggggccgc agtacaccga gctgcacgcg
     4981 gggccaccca agggcggatg gccggtcggt ccgttccagg tgacggttta tgtcgacgga
     5041 gactcggtcg acgccaagac gttcgcggtg gacaagcctt ccgacacagg aaaggccgtg
     5101 gggggagata cagcagccaa gaccggagat tcgacgagca cgcagtgacc tctcggccag
     5161 gtccggcgtc gccgttgcgg ggtgccggcc tggcttcttt ctcctcactc ggcgtatagt
     5221 acgacgtgac cgctcactgg attctcaaga ccgagccctc gacctactcc ttcgatcgcc
     5281 ttctcgaaga acggaagaca gtgtgggacg gagtaagcaa tcccgtcgcg ctcaagcatc
     5341 ttcgtgagat gacggttggt gatcaggtca tgatctacca caccggcgat gtgaaggcgg
     5401 tcgtgggctt ggcccggatc acccgcgcgg cttatcccga ccccaaggat tccaagctcg
     5461 tcgtcgtcga gatcgaggcc ggcgagcgac tcccgcaccc ggtcacgctc gctgcgatca
     5521 aagccgatcc cgcgttcgcg gatctcgctc tggtccgcat gggccgtttg tccgtggtgc
     5581 cggtaaagcc ggagcagtgg aagcagatca cccagatggc ggaagagcag ggagagggag
     5641 aaaggggaaa gggagaaaag ggaaaaggag aaaggggaaa ggggcgggga ggctagggcg
     5701 ctgctccatc cgtttccctt ctagcccttc ccccttcccc ctttccccta ttcccctttc
     5761 cgcgctcctg aatggatccc gaacccccgc ttatattgcg ggatgtcccc ccgcttcgaa
     5821 gtggtcgcgc cgttcgagcc ggccggcgat caacccaagg caatcgacga gctgactggt
     5881 gggctgcgcc gaggcgagcg gtatcagacg cttcttggcg tgaccggttc cggcaagacg
     5941 atgacgctcg ctcacgccat cgccgcccac cagaagccga cgctggtcct ctcgcataac
     6001 aagacgttgg cggcgcagct ctacggagag ctgcggcaat tcctgcctgc caacgctgtg
     6061 gagtacttcg tctcgtatta cgactactac cagcccgaag cgtacgtccc ggcgaccgac
     6121 ctttacatcg agaaggacgc ctccatcaac gaggacatcg agatgctgcg cctccgcgcc
     6181 acctcgagtc tgatggagcg cgaggacgtg gtaattgtcg caaccgtgag cgcgatctac
     6241 ggcttaggcg atccgctaga gtatcgccag ctcatggtga gcgtgaagcg gggagagcgc
     6301 cggggacgcg actccattct ggcggagctg gtgcggatcc agtacggccg caacgatgcg
     6361 tcgttcgaac cgggcacgtt ccgggttcgg ggagatagtc tggagatcta tcccgcgtat
     6421 gccgagcagg cagtgcggat cgagctctgg ggcgacgagg tcgagcggat cagcaagatc
     6481 aatccgctca ccgggcagac cattgcgcag ctggatcaga tggccatcta ccccgccaag
     6541 catttcgtca cacagcggcc caccatcgag cgggcggtaa agctcatccg cgcagaactg
     6601 gccgaccggc ttcagctctt ccgcagtcag ggcaagctgc tggaggcgca acggctcgag
     6661 tcgcgcacca acttcgacat cgagatgctg ctcgagatcg gcacatgtgc cggcatcgag
     6721 aattactcgc ggccgctctc cggccggcag gagggagagc gacccgccgt cttgctggac
     6781 tacttcccgc ccgactttct ggtggtggtg gacgagtcgc atgtgacgct gccgcagctc
     6841 ggcggcatgt acaacggcga tcgggctcgc aagctcacgc tggtggagta tgggttccgg
     6901 cttccctcgg cgctcgacaa ccggccgctc cagttcgacg agttcatgag tctggtgccg
     6961 cagatggtga atgtctcggc gacgccgggt gatttcgagc tgcgcctct
//
LOCUS       DASRJQ010000277         8757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2496726, whole genome shotgun sequence.
ACCESSION   DASRJQ010000277 DASRJQ010000000
VERSION     DASRJQ010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 8757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8757
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2496726"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VFB89_06200"
     CDS             complement(<1..366)
                     /locus_tag="VFB89_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26927.1"
                     /translation="MEARKSDWIAWPVAWSAVWVGALTALAVGLIIGLLGYALGANEV
                     AGYVDWKKVRLITLIFSIGGAFFAFVAGGWAAGRVAGIRRSEPAILHGAIVWLVAIPL
                     LLIFAALGAAGHFGNWYGGL"
     gene            561..914
                     /locus_tag="VFB89_06205"
     CDS             561..914
                     /locus_tag="VFB89_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26928.1"
                     /translation="MSLAMARIRLLAGDAYRLGCRDKSDPSHQAGRELVNQALRDLEL
                     VLQGIRDAAAIEHSQRRANDTAAWLRAALLALPRETSDDFAAGWRGAVLRAAEHAFGV
                     GSPEVEALERLSRCG"
     gene            1035..1535
                     /locus_tag="VFB89_06210"
     CDS             1035..1535
                     /locus_tag="VFB89_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26929.1"
                     /translation="MQLAGTYDMTHFLVVVEAVLVGVLGGAVFFRGRRWWGYRLLVPI
                     VLALVICELAPVLLQVSNPAGEVAIAGLLLAQGTLLWGMLRKEQADRAELLQRIRLML
                     QDRVRNKLQVIAGATNGRTDVFRALEEIAQSIDELNDGTLVKWEDGYTEPLKQAKLTR
                     PVADSA"
     gene            complement(1784..2212)
                     /locus_tag="VFB89_06215"
     CDS             complement(1784..2212)
                     /locus_tag="VFB89_06215"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZM26930.1"
                     /translation="MTLLLLAACERGTNRAPAVDYIPVAGNPQLPFSDAVRVGHMLYL
                     AGQLGTDSTGHLVSGGIAGETRQALENIRRVLERNGSSLDQVVKCTAMLADIQDWGAM
                     NQVYVTYFRKHLPARSAFATTGLVMGARIELECWATVTPK"
     gene            complement(2246..3955)
                     /locus_tag="VFB89_06220"
     CDS             complement(2246..3955)
                     /locus_tag="VFB89_06220"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HZM26931.1"
                     /translation="MITIVNHHPARSAARTDTQPRFAAGETVDFAIVGAGAAGGVVAR
                     ELARVGFTVVVLEQGPHLQKEDFHHDELAITALSALTNDPKTQPNTFRSSESDEAQVR
                     PVVQYGRLVGGGSVHFTGNYWRMHEIDFVERSRKGSIEGTGFTDWPITYAELEPYYSK
                     VEWEMGVSGLAGSSPFDPPRSRPYPLPPLPVKPAGVLAERAARKLGWHAFPAPMAILS
                     QPYRGRPPCIQCGFCKTFGCEVGAKSSTLAAMFPDAAATGRCEIRPVSYVRKIELDKR
                     GHVTGVKYFDSGKREMFQRARAVVVCANGAETPRLLLMSRSKLFPDGLANSSGVVGKH
                     LMFNGYAFAGGLFEHRINGYRGMVVTRVIHDLYELDPRLGIAGGGGFDFRFDWGPIGF
                     ALEGLPKEAPNWGPEYKGMLRDYYIRSVYVLAHATQLPMPNNSISLDPKLTDAWGLPA
                     IRMTFADHPNDLKLYAYLLDRAIELLDAAGATRRWAYPLERVFPEVHLLGTCRMGNDP
                     ATSVVDRFNRTHDIPNLFLVDGSSLVTSGRGQPTMTIQALAFRAAEHAARLARRGELS
                     KRS"
     gene            complement(3924..4484)
                     /locus_tag="VFB89_06225"
     CDS             complement(3924..4484)
                     /locus_tag="VFB89_06225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HZM26932.1"
                     /translation="MSQGPLSRRHFVGIMAGAVAATWITGAPRLNVSGIGASGSVSGV
                     LSAAQRRDLDALTALIIPTDEDAGAREARIVDFIDRGLTSFAGDQRTLFEQGLTDLES
                     RAQRSGGRAFADLDPAAQLALARELDAGKSEFFGAVRVATIAGFLANPEYGGNTGKVG
                     WQLIGFEDRFGWQPPFGDYDREPSPG"
     gene            4709..6274
                     /gene="kaiC"
                     /locus_tag="VFB89_06230"
     CDS             4709..6274
                     /gene="kaiC"
                     /locus_tag="VFB89_06230"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF006799.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HZM26933.1"
                     /translation="MTKDGRRGAARTGSKRVRQSSGRRSIAKAPTGIAGLDEITRGGL
                     PRRRATLVCGGPGSGKTLLGTQFLAHGAMVLDEPGVFVSFEEPPHELIENSRSLGIDL
                     RDLVSRGKIFIDYVRVERSEIVESGGYDLDGLFIRLAHAISSIGAKRVVLDTIEMLFS
                     GLTDAFILRAELHRLFQWLKTKGVTTIVTGERGTDGLTRHGLEEYLTDCVIVLDQRVT
                     AQEATRRVRILKYRGSAHSNNEIPFLIDDNGLSVLPISSLGLHYDAPTGSVSTGIDDL
                     DGLFSNGGYFRGSSVLVSGQAGTGKSSLAAHFAHAAVQRGERSLYLAFEESAQQIVRN
                     MRSIGLDLESDVRKSLLQIHAQRATHLGLEAHLVAIHRLINEWHPTVVVLDPVSNLTN
                     IASPLEVKSMLSRLLDFMKGLEITALATSLTGGDSTSYQSDIGVSSLMDVWMVLQNLE
                     ANGERNRALQIIKARGTSHSNQIREFVLTDEGLKLAPIHRRLDGRVLVGTSREVQQDA
                     EAAMQPAAPGARS"
     gene            complement(6433..6630)
                     /locus_tag="VFB89_06235"
     CDS             complement(6433..6630)
                     /locus_tag="VFB89_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26934.1"
                     /translation="MIDCARLGSAQRCSARPGWGQAETYENALPARKIANDLAHRTGE
                     LAYQRGNGNDLILASTMRVFQ"
     gene            6629..7840
                     /locus_tag="VFB89_06240"
     CDS             6629..7840
                     /locus_tag="VFB89_06240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZM26935.1"
                     /translation="MKTSPASSTTQTRLEKLRKRLRTAEDTLRAIQAGEVDAVVTGSR
                     SKRVVTLAGAELPYSIMFDQMNEGAVTLTRDGTVAYCNRRFAEMVQATPERIIGTSLQ
                     QFVAPRDHTALIALQKSAGKGSARGELSLVCPDGVPVPVSVSFAPLQHAAARIGLIAV
                     VADIRERRQAEALQARLTAQVMTAQDEERRRVARELHDDTGQTLTGLLVGLRTIEASD
                     TVTEAREMAEQLRGIAGEGLNNLRRMVSGLHTAALDELGLNAAVTRHVHQFSELHGLP
                     VTLQVVGVKPDGLPPLVQNTVYRVLQEALTNIAKHSRARAARVRLSWRKAMVELQVKD
                     NGVGIRQHSRGAPGRGLGLKGMRERAALLGGVIEIDSTPSEGTTINCRIPLDSAMLSA
                     NRRKPVKARSP"
     gene            7844..8512
                     /locus_tag="VFB89_06245"
     CDS             7844..8512
                     /locus_tag="VFB89_06245"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZM26936.1"
                     /translation="MSKVRVLIADDHSVVRAGLRMLIASQPDMEVVGEAGDGAEAIAK
                     AGSTHPDVVVLDLSMPKSRSTQTIERIVRLENQPRVLILTMHDDPAYFRSAFDAGASG
                     YVVKNAADLELLTAIRAVYRYRTFVDLSRMKEVPFVASPSGSALAKPKKKLSRREAEV
                     LRLLAQGYTNQETAEKLAVSVKTVETYRKRLHEKLELKTRAQLFRFAFESGLLESDAN
                     LPDE"
BASE COUNT         1670 a         2764 c         2786 g         1537 t
ORIGIN      
        1 caaaccacca taccagttgc cgaaatgccc ggccgcacct aacgctgcaa agatcaggag
       61 caagggaatc gccacgagcc acacgattgc accatgcagg attgccggct ccgagcgacg
      121 aattcctgct actcggcccg ccgcccaccc gcctgcgacg aatgcgaaga aggccccccc
      181 gatgctgaaa atcagagtaa tgagccgaac cttcttccaa tcgacgtagc cagccacttc
      241 gttggctccg agcgcgtatc ccagcagacc aatgatcagt ccaaccgcaa gcgcggtcag
      301 cgcgccaacc cagactgcgc tccacgcaac cggccatgcg atccagtccg acttgcgagc
      361 ctccatacgt gcgttccttt ctccggcggt catccgattg gcccggcacg atagaatccg
      421 gttccacctc actgccgtgg cagtgctcac ccggagaagc caatggtcct cactcccttg
      481 ccaccgaaga gaacccgaag catgatgtaa gcatgctacc gcgaaaccag gtttgggagg
      541 catccggccc gggcgccgcc atgtccctcg ctatggcgcg tatccggctg ctggccggcg
      601 acgcgtaccg gcttggctgc cgtgacaagt cagaccccag ccatcaggct ggtcgggagc
      661 tggtgaacca ggccttacgt gacctcgagc tggtgctcca gggaattcgg gatgccgcgg
      721 ccatcgagca ttcccaacgg cgcgccaacg acacggcggc ctggctgcga gccgcactgc
      781 ttgccttgcc acgggagacg tccgatgact tcgcggcggg atggcgcggc gccgtgctcc
      841 gggccgccga gcatgccttt ggggtgggaa gtccggaggt agaagcgttg gagcgattga
      901 gtcggtgcgg ctaattgcag gtgactaccg aaaaggcggg ttgctgacaa tctttgggca
      961 ctgggacgtg ccgactctct tcgccgaatc accgtgcact cgacgcgatt tcatacgaac
     1021 tgagacctcc tacaatgcaa ctcgcgggca cctacgacat gacgcatttc ctggtggtcg
     1081 tggaggcagt attggtgggc gtgcttggcg gagcggtttt cttccgcggt cgtcggtggt
     1141 ggggctatcg cctcctggta ccgatcgtgc tcgcactcgt gatctgcgag ctggcacccg
     1201 tcctgcttca agtcagcaat ccggcaggtg aagtcgcgat cgccggcctg ctgctcgccc
     1261 agggcacctt gctgtggggc atgctgcgca aggagcaggc ggatcgggca gagctgctcc
     1321 agcgaattcg cctcatgctc caggatcggg ttcgcaacaa gcttcaggtc atcgccgggg
     1381 cgacgaacgg acggacagat gtttttcgag cccttgaaga gatcgcgcag tcgatcgacg
     1441 agctcaacga tgggacgttg gtcaagtggg aggacggata cacggagccg ctcaagcagg
     1501 caaagctcac aagaccggtt gcagattcgg cctgacagcg tcggcgccgc gttgcggcgc
     1561 cgatttcaca tcgtgctgat cttcacataa cggcgaaggt cgggaaggct gaatgcgatc
     1621 agagcgccga tcccaaccat caagccccac aaggcaacgc gtcgcattca atcacctcat
     1681 ttgtcggagt cttccccatc atggggacgc gcctgtcagc gcgatgtgat ggctggccag
     1741 agatccagct caaacatcag ttttcagggt cgagccgagc ggctcatttc ggggtcacgg
     1801 tagcccagca ctccaattca attcgggctc ccatcaccag tcccgttgtc gcgaaagcgc
     1861 ttcgcgccgg cagatgcttc cggaagtagg tcacgtagac ctgattcata gcaccccagt
     1921 cctgaatgtc cgcaagcatc gcggtgcatt tgacgacctg gtccaacgac gatccgttcc
     1981 tttccagaac ccggcggatg ttctccaggg cctgacgggt ctccccagcg atcccgccag
     2041 ataccagatg gccggtggaa tcagtgccaa gctgtcccgc caggtacagc atgtggccga
     2101 ctcgcactgc gtcggagaaa gggagctgcg ggtttccggc cacgggtatg tagtccaccg
     2161 ccggagcccg attcgtccct cgctcgcacg cggccagcag aagcaacgtc atcagtcgag
     2221 ccacccttga tgccatctgc attgctcaac tccgcttcga gagttcgccc cggcgcgcca
     2281 gccgggcggc atgttccgcg gcacggaagg ccagggcctg gatcgtcatg gtaggctggc
     2341 cgcgcccgga cgttaccagg ctgctgccat caaccaggaa gaggttgggg atgtcatggg
     2401 tccggttgaa tcgatcgaca accgatgtcg ccgggtcgtt ccccattcga caggtgccga
     2461 gcaggtggac ctccggaaag acgcgctcga gggggtacgc ccagcgccga gtggcgccgg
     2521 ccgcgtcgag cagctcgatg gcgcgatcca ggagataggc gtagagcttg aggtcgttgg
     2581 gatgatcggc aaaggtcatc cggatggcgg gcaggcccca ggcatcggtc agcttcggat
     2641 cgagcgaaat cgaattgttc ggcatgggaa gctgagtggc atgcgcgagc acgtacaccg
     2701 atctgatgta atagtcgcgc agcatcccct tgtactcggg tccccagttg ggtgcttctt
     2761 ttggaagccc ttccagggca aacccgatag ggccccagtc gaaccggaag tcgaagcctc
     2821 cgccgccggc gatcccgagc ctgggatcga gctcgtagag gtcatggatc acccgggtca
     2881 ctaccatccc acgatacccg ttaatgcgat gctcgaagag tccgccggca aacgcgtagc
     2941 cgttaaacat gagatgcttg ccgacgacgc cgctcgagtt ggccaggcca tcgggaaaga
     3001 gcttggatcg tgacataagc agcagccgcg gcgtttccgc gccattggca cacaccacca
     3061 ccgcccgggc ccgctggaac atctctcgct ttcccgagtc gaagtacttt acgccggtga
     3121 catggccccg cttgtccaac tcgatcttgc gcacgtagct caccgggcga atctcgcatc
     3181 ggccggttgc cgcggcatcg gggaacatgg ccgcgagcgt gctcgacttg gcccccacct
     3241 cacagccgaa ggtcttgcag aagccgcatt gaatgcacgg cggacgtccg cgatatggct
     3301 gtgagaggat cgccattgga gcagggaaag cgtgccaccc cagtttgcga gcggcccgct
     3361 ctgcgagaac gcccgcaggc ttgaccggaa gcggtggcaa ggggtacggt cggctgcggg
     3421 gtggatcgaa ggggcttgag cccgcgagtc ccgacacgcc catctcccat tcgaccttgc
     3481 tgtagtaagg ctccagctcg gcataggtaa tgggccagtc ggtaaagccg gtcccctcga
     3541 tcgagccttt gcggcttcgc tccacgaaat cgatctcgtg catgcgccag taattcccgg
     3601 tgaagtgcac cgacccaccg ccgacgagcc ggccgtactg gacgaccgga cgaacctggg
     3661 cctcatccga ttcggagctg cggaacgtgt tgggctgggt cttggggtcg ttggtgagag
     3721 cgctcagcgc ggtgatggcc agctcgtcgt ggtgaaaatc ctccttctgg aggtgaggcc
     3781 cctgctcgag caccaccaca gtgaacccca cgcgtgccag ctcgcgcgcg acgaccccgc
     3841 cggccgctcc ggcgccgacg atcgcgaagt ccaccgtttc gccggccgcg aaacggggct
     3901 gagtgtcggt ccgggccgcg cttctagccg ggtgatggtt cacgatcgta atcaccgaag
     3961 ggaggctgcc acccgaaacg gtcctcgaag ccgatcagct gccagccgac cttacccgtg
     4021 ttcccgccat actccggatt cgcgagaaag ccggcgatcg tcgccacacg gaccgcgccg
     4081 aagaactccg atttcccggc atccagctca cgcgccagcg ctagctgggc tgcgggatcc
     4141 agatcggcga acgcgcgtcc accggagcgc tgggcgcggc tctcgaggtc ggtgaggccc
     4201 tgctcgaaga gggtccgctg gtccccggcg aatgaagtga ggccgcgatc gatgaaatcc
     4261 acgattcggg cttcgcgcgc acccgcgtcc tcatccgtcg gaatgatgag tgccgtaagc
     4321 gcgtcgaggt ctcggcgctg agctgcggac aagactcccg acaccgaccc gctcgccccg
     4381 atgccggaca cattcagccg aggggcgccc gtaatccagg tagccgcgac cgctcctgcc
     4441 atgatgccaa cgaagtgccg ccgtgacagc gggccctggc tcatgcgtgc gcaaatcgat
     4501 gaggaaggtg ggcaattctg ccactcgctg gggaccttgt ccagccggtg ccctggcggc
     4561 gaggcacgga gccatctcta gcgggggcct ccaagggcaa ttaggttcgg tgcggacaga
     4621 ctccgcgtct caaactgcag gtcgtttact caagcagaga tagggcagct cgcctcacca
     4681 agcctacgca gagcagggac cctcgcagat gaccaaggat gggcgtcggg gcgccgcgcg
     4741 gaccggaagc aagcgggtgc gccaatccag cggacgccgg tcgattgcca aagctccgac
     4801 aggaattgcc ggactcgacg agatcacccg cgggggtctg ccgcggcgcc gggcgacgct
     4861 cgtgtgcggc gggcccggct ccggcaagac tcttctgggc acgcagttcc tcgcgcatgg
     4921 tgcgatggtt ctggacgaac cgggggtttt cgtctccttc gaagagccgc ctcacgagct
     4981 gatcgagaac tcgcgctcgc tggggatcga tctgcgcgac ctcgtgagtc gcggcaaaat
     5041 ctttatcgac tacgtgcggg tcgaacggag tgagatcgtc gagagcggtg gttacgatct
     5101 cgatggcctg ttcatccgtc tggcccatgc gatcagctcg atcggtgcca aacgggtcgt
     5161 gctggacacg atcgaaatgc tgttctccgg cctgacggac gcgttcattc tgcgggccga
     5221 gctgcatcgg ctattccaat ggctcaagac caagggcgtc acgacgatcg tcaccggcga
     5281 gcggggaacc gatggcctga cgcgtcacgg tctggaggag tacctgaccg actgcgtcat
     5341 cgtgctcgac caacgcgtga ctgcgcagga agccacccgt cgagtccgca tcctcaagta
     5401 ccgcgggtca gcgcacagca acaacgagat tccctttcta attgacgaca acgggctgtc
     5461 ggtcctgccc atcagctcat tgggactgca ttacgacgcg ccgaccggca gtgtatccac
     5521 cgggatcgac gaccttgacg ggctgttcag caacggaggc tatttccggg ggagcagcgt
     5581 tctggtctcg ggccaggccg gcaccgggaa gagcagtctg gccgcccatt ttgcgcacgc
     5641 tgcggttcag cggggcgaac gcagtctcta cctcgccttc gaagagtcag cccagcagat
     5701 cgtgcgcaac atgcgctcga tcggcctcga cttggagtcc gatgtgcgga agagtcttct
     5761 ccagatccac gcccagcggg caacccatct cgggctcgaa gcgcacctgg tggcaatcca
     5821 ccggctgatc aacgagtggc atcctacggt cgtagtcctg gacccggtca gcaatctgac
     5881 caacatcgcg tcgccgctgg aagtgaaatc gatgctgtcg cgcctcctcg acttcatgaa
     5941 gggcctcgaa atcacggccc tggccacgtc gttgaccggt ggcgacagca cttcgtacca
     6001 gtcggacatc ggggtgtcct cgttgatgga tgtctggatg gtactgcaga acttggaggc
     6061 aaacggcgag cgcaaccggg cactgcagat catcaaggcc cgggggacga gccattcgaa
     6121 tcaaatacgg gaattcgtac tgaccgacga ggggctgaag ctggccccaa tacaccggcg
     6181 gctcgacggg cgcgtactgg tgggcacatc tcgagaggta cagcaggacg cggaggcggc
     6241 aatgcagcct gccgccccgg gggcccggtc atgaccagcc ctctgcgccg tcgcagccct
     6301 ccgcgccgac gaacctggcg cctccggctg tatgtggccg gtcagacacc gcgatccatc
     6361 accgcgctgg acaacttgaa gcgcctctgc gatgccgaca tcggaaacag atatgaaatc
     6421 gaggttatcg atctactgaa acacccgcat cgtgctcgcc aggatcaaat cgttgccgtt
     6481 cccacgctgg tacgcaagct cccccgtccg atgcgcaaga tcattggcga tctttcgagc
     6541 agggagcgcg ttctcgtagg tctcggcttg cccccaaccc ggtcgcgctg agcagcgctg
     6601 agcgctcccc aaccgagcgc agtcgatcat gaagacctca cccgcatctt cgaccaccca
     6661 gacacgactg gagaagcttc gcaagcggct gcgcaccgcc gaagatacgc tccgcgcgat
     6721 tcaagcgggc gaagtcgatg ccgtggtgac cgggtcccgc agtaagcggg tcgtcacgtt
     6781 ggcgggagcc gagcttccct acagcatcat gttcgaccag atgaacgagg gcgccgtcac
     6841 cctgacccgt gatggcaccg tggcctactg taaccgccgc tttgccgaga tggtgcaggc
     6901 cacgcccgag cgcattatcg gcacgtcgct gcagcagttt gtggcacccc gagaccacac
     6961 cgccctcatc gctctgcaaa aaagcgccgg gaagggaagc gcgcggggtg aactgagcct
     7021 cgtttgcccg gatggcgtgc ccgtccctgt ctcggtttcg ttcgcacccc tccagcatgc
     7081 cgccgctcgc atcggcctga ttgccgtcgt ggcagacatt cgggagcgca ggcaggcgga
     7141 ggcattacag gcgcgcctga cggcccaggt catgacggct caggacgaag agcgacggcg
     7201 cgtcgcgcgc gagctgcatg acgacacggg gcagacctta acgggcttat tggtcggctt
     7261 acgcaccatc gaggcatcgg atacggtgac cgaagccaga gagatggccg aacagcttcg
     7321 cgggatcgcc ggtgagggcc tcaacaacct tcggcgaatg gtctcgggcc tgcacacggc
     7381 tgcgctggac gagctcggcc tgaatgcagc cgtcacccgg cacgtgcatc aattttccga
     7441 gctgcatggg cttccggtca ctctacaggt ggtcggggtc aagccggacg gcttgcctcc
     7501 cctggtacaa aacacggtgt atcgagtcct gcaggaggct ttgaccaaca tcgccaagca
     7561 ctcgcgcgcg cgcgccgcgc gagtgcggct ttcctggcgc aaggcgatgg tcgagcttca
     7621 ggtcaaggac aatggggtgg gtatccgcca gcattccagg ggcgcaccgg gccggggtct
     7681 cggactgaag ggcatgcggg aacgggcagc cctgctcggc ggtgtgatcg agatcgactc
     7741 gaccccgagc gaagggacga ccatcaattg tcgcatcccg ctcgacagcg ccatgttgtc
     7801 cgcgaatcgg cggaagccgg tcaaggcgcg gagcccgtag tcgatgtcca aggtgagggt
     7861 gctgatcgcg gacgaccatt cggtggttcg ggccggcctt cgcatgctga ttgcgagtca
     7921 gcccgacatg gaggtcgtag gcgaagccgg cgatggggcc gaggcaatcg ccaaggccgg
     7981 ctcgacgcat ccggacgtgg tcgtcctcga cctctcgatg cccaagtcgc gcagcactca
     8041 aacgatcgag cgcattgtcc ggcttgagaa ccagccccgg gttctgatcc tcaccatgca
     8101 cgatgacccc gcctacttcc ggtcggcgtt cgacgcgggg gcatcgggat atgtggtgaa
     8161 gaatgcggcc gatctcgagc tgctgactgc gatccgcgcc gtgtaccgct accgcacctt
     8221 cgtcgatttg agtcggatga aggaagtacc gttcgtggca tcacccagcg gcagcgctct
     8281 ggcgaagcca aagaaaaaac tcagccggcg ggaggctgag gtgcttcggt tactggcgca
     8341 gggatacacc aatcaggaaa ccgcggagaa gctggctgtc agcgtgaaga cggtggagac
     8401 atatcggaag cggttgcatg agaagctgga gctgaaaaca cgggcgcagc tgttccgctt
     8461 tgcgttcgag tcggggttgc tggagtcgga tgccaatctt ccggatgagt agcccgccgg
     8521 gtctgtcagg gtttgcctga catcttgcct gggcgggagc cttttcccga tacccttgat
     8581 atcggcgatt cgtatcttct caccacgatc tcaggtgatt cacgagcgag cgaccagcta
     8641 atcctcgagc ggtgccggtc gccgtagagt aatgcggcag agtgcccgac gcaggcgcgg
     8701 ttcgtaccca tggtgtgcgg taaggcgcca caatacatcg taactgtcag gaggaag
//
LOCUS       DASRJQ010000278         4861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3836616, whole genome shotgun sequence.
ACCESSION   DASRJQ010000278 DASRJQ010000000
VERSION     DASRJQ010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3836616"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..762
                     /locus_tag="VFB89_06250"
     CDS             106..762
                     /locus_tag="VFB89_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HZM26937.1"
                     /translation="MNVVATYPIADIFHSLQGEGHFVGYPMTFLRLAGCSVKDCHIRA
                     ECDEAPWKMRERLTLDEIVDRVRGLQPSGIVCITGGEPTDHDILPMIAALQTAGYRIH
                     LETSGVRSVAGYPLEWLTVSPKVFDFGGLVQKSGHTLKIVVRPEWDATRAWDIINGAS
                     DCTDFFHRYMQPMTGADGQPLNLPQVVDMLLNRSGPCGRNGNAKWALSVQAHRNWGLK
                     "
     gene            791..979
                     /locus_tag="VFB89_06255"
     CDS             791..979
                     /locus_tag="VFB89_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26938.1"
                     /translation="MSPAEQIEITETTEVCVWPNKPPTVSLHGFETTDDMTLDEVRKL
                     WRRRGLRARGDGARHSRP"
     gene            1068..1301
                     /locus_tag="VFB89_06260"
     CDS             1068..1301
                     /locus_tag="VFB89_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26939.1"
                     /translation="MTPEEQDKILNVIAIADGGCSNCVSGLLDALGKVFPDVPWRKSF
                     GPFSYDTINKRGGVVNKLMWPDAYDDEDEAGDS"
     gene            1317..1550
                     /locus_tag="VFB89_06265"
     CDS             1317..1550
                     /locus_tag="VFB89_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26940.1"
                     /translation="MSDQRGIVHSAGQTPYRRGDIRLMVGFCTLYEEGHTVLLTLDQG
                     DQSEVEIALLPAEARALAAKLVEAADSMDKPTG"
     gene            complement(1547..2320)
                     /locus_tag="VFB89_06270"
     CDS             complement(1547..2320)
                     /locus_tag="VFB89_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26941.1"
                     /translation="MRQVVKEAWLEFTEPLEGGLNYLYADKLNLITTGYGNLVDPLSM
                     AMALPWMIDGRAARPDEIATAWWTIKNDPNSAWRGHTYAKTLSALRLLPEAVHDLVYG
                     KLRSNDAELGEAFAEFEEWPAMAQMAMHSWAWACGAHSPFPELFKALRKQDFAAAAVH
                     IHINEDGADRIHGTADDNRGLIPRNVANKILMRNAAKVLAFKLDPDMLEWKRDLGPPD
                     ITTAPELENPASEPTICVGPIAHVDPSDYLRNRDDEDDS"
     gene            complement(2317..2466)
                     /locus_tag="VFB89_06275"
     CDS             complement(2317..2466)
                     /locus_tag="VFB89_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26942.1"
                     /translation="MGVLTLVMRLLEVVSALLKANGDHVAEEEAMMIGQEVLKAELDR
                     RKFGP"
     gene            complement(2466..2693)
                     /locus_tag="VFB89_06280"
     CDS             complement(2466..2693)
                     /locus_tag="VFB89_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26943.1"
                     /translation="MISFRKVLAIALALFGALFVIAKCTGCAPSTARPAAYGAELTAC
                     TTTSATLEASIACENGVRARYGRPLRDAGGW"
     gene            complement(2690..3223)
                     /locus_tag="VFB89_06285"
     CDS             complement(2690..3223)
                     /locus_tag="VFB89_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26944.1"
                     /translation="MVDDMPPQPKTAQLAAADKNEILLAEIKTLMTQGLTALDNKVAI
                     GFRNLDEKVDRLEANQDLQGGELGLVKKEVALIFEWKGDVDQRLKNNSHRAASASEVD
                     LTHESRLAQTKAELDAEKAAREALATKVDTALGLLTSVESGIKTLSRNPTVKRIGRYA
                     IAILIGWMASKGIKVPF"
     gene            complement(3235..3960)
                     /locus_tag="VFB89_06290"
     CDS             complement(3235..3960)
                     /locus_tag="VFB89_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26945.1"
                     /translation="MLARMVRPVGAPVFDPATLVLSAWWRANYVTPPWGQNASAGTSG
                     GNDPLDEATDYPTSGTAVNGLTPAAFNGSTQFLGTAGAFSTYATDTAYSGWVLMKVTS
                     ISTDIAAPSLYDNNNVWSNGPYAGLVLRDSTGTKSATLFHFDGAQQGVSTAIATGAWT
                     LVQWKYDGTNIKIRTNGGSYSSTAAGTLGDLTAPFLVSQAYDGTKSFTGSILEIGLSL
                     GVISDANFDNIKSYVNSRYALAL"
     gene            complement(3960..>4861)
                     /locus_tag="VFB89_06295"
     CDS             complement(3960..>4861)
                     /locus_tag="VFB89_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26946.1"
                     /translation="LSVRIANDVTGLGTGVATFLATPSGANLASALTTALPDTKGGTG
                     LTALGTGVATWLGTPSGANLASALTTALPDTKGGTGLTSLGTGVATWLGTPSGANLAS
                     ALTTALPETKGGTGLTALGSGVATALGTFSSANIKTACTDETGSGGALVFATSPTIDA
                     PTLSGLPVLSGNSASTSNNAKGTAVDVNPVNVQTTDATVTTLDSFTIASNSAVVVSWL
                     VTAIKSDSSQAAAYSVTACFRNAAGTAAQVGTSTTTIIGESDSVWDATIDNSTTTIRL
                     RITGKAATTIQWTSILTRLTVIP"
BASE COUNT          845 a         1521 c         1587 g          908 t
ORIGIN      
        1 ctacgacccc gtggacggcc tggcgtgcgg ccagtgtgac tcctgccgca tccgtcagga
       61 cgggttcaag gctgcgggca ccccggatgt gacgaggtac gcagcgtgaa cgtcgtggct
      121 acctacccga tcgcggacat cttccactcg ctgcagggtg aggggcactt cgtcgggtac
      181 ccgatgacgt tccttcgcct ggcggggtgc agcgtgaagg actgccacat ccgggcggag
      241 tgcgacgaag caccgtggaa gatgcgcgag cgcttgaccc tggacgagat cgtcgaccga
      301 gttcgcggtc tgcagccgag cggcatcgtt tgcatcaccg gtggcgaacc gaccgaccac
      361 gacattctgc cgatgatcgc ggcgctgcag acggcgggct accgcattca cctcgaaacg
      421 agtggcgttc gatccgtcgc cggctacccg ctcgagtggc tcacggtgag cccgaaagtg
      481 ttcgacttcg gcgggctcgt gcagaagagc gggcatacgc tgaagatcgt ggtgcgtccg
      541 gaatgggacg ctacgcgggc atgggacatc atcaacggtg cgtccgactg caccgacttc
      601 ttccaccgct acatgcagcc gatgaccggc gcagacggcc agcccctgaa cttgccgcag
      661 gtcgtggaca tgctcctcaa caggagcggt ccgtgcggcc gaaacggcaa cgccaagtgg
      721 gcgctgagtg tccaggctca tcgaaattgg gggctgaaat gaaggcgacg cagaagggaa
      781 gcgcaaccaa atgagcccgg cagagcagat cgagatcacc gagacgacgg aggtttgcgt
      841 gtggccgaac aagccgccga ccgtgagcct tcacgggttc gagaccaccg acgacatgac
      901 gctcgacgaa gtgcgcaaac tttggcgccg ccgtggcctt cgggctcgtg gggatggcgc
      961 acgccattcg agaccgtaac gcctgcccca agtgcggggc gaacatgctt ggagagggag
     1021 ggccctgcta tgactgcgta cgctacgcct gacaggagcg aagaccgatg acacccgagg
     1081 agcaagataa gattctcaac gtgatcgcca tcgcggacgg cgggtgctcc aactgcgttt
     1141 ccggcctgct agatgccctc ggtaaggtct tcccggacgt gccctggcgc aaaagcttcg
     1201 ggccgttcag ttacgacacg atcaacaagc gcggtggcgt cgtaaataaa ctgatgtggc
     1261 ccgacgcgta cgacgacgaa gacgaggcgg gtgattcgtg aagcaaggac aaaactatga
     1321 gtgaccaacg tggaatcgta cacagtgcag gccagacacc gtaccgccgt ggcgacatcc
     1381 gcctgatggt cggcttctgc acgctatacg aggagggcca tacggtcctt ctgaccctgg
     1441 accaggggga ccaaagcgag gttgagattg ccctcctccc ggccgaggcc agggcgctgg
     1501 cagccaagct agtcgaagcg gccgattcga tggacaaacc gacgggttag ctgtcgtcct
     1561 cgtcgtcccg gttcctgagg tagtcgctcg ggtccacgtg cgcgatcggc ccgacgcata
     1621 tagtcggctc gctggctggg ttctccagct ccggggcggt ggtgatgtcg ggcgggccta
     1681 ggtcccgttt ccactccagc atgtcggggt ccagcttgaa ggccagcacc ttggcggcgt
     1741 tgcgcatcag gatcttgttt gcgacgtttc gcgggatgag cccacggttg tcgtccgcgg
     1801 tcccgtggat gcgatccgca ccatcttcgt tgatgtggat gtgaaccgcc gccgcggcaa
     1861 agtcctgctt tcgcagggcc ttgaacagct ctggaaacgg cgaatgcgcg ccgcatgccc
     1921 aggcccacga gtgcatggcc atctgagcca tcgcgggcca ctcctcgaat tcagcgaacg
     1981 cctcgccaag ctccgcgtca ttcgaccgca gctttccgta aacgaggtcg tgcaccgcct
     2041 ccggaaggag acgcaaagcg ctgagcgtct tggcgtaggt gtgcccacgc caagcgctgt
     2101 tcgggtcatt cttgatggtc caccatgccg tcgcgatctc gtccgggcgg gctgcgcgac
     2161 cgtcaatcat ccacgggagc gccatcgcca tcgacagcgg gtccacgagg tttccgtatc
     2221 cggtggtgat gaggttgagt ttgtccgcgt agaggtagtt taggcccccc tcgaggggtt
     2281 ccgtgaactc cagccacgcc tcctttacga cctgcctcat ggcccaaact ttcggcgatc
     2341 gagctcggcc ttcaggacct cctggccaat catcatcgcc tcctcttccg ccacgtggtc
     2401 cccgttcgct ttgagcagcg cgctgacaac ctccagcagc ctcatcacca gcgtcaggac
     2461 gcccatcacc accctcccgc gtcgcgaagc ggacgcccgt agcgggcacg gacgccgttc
     2521 tcacaagcaa tgcttgcctc caaggttgcc gaggtggtcg tgcacgccgt gagctctgcg
     2581 ccgtacgcgg caggccgcgc cgtcgacggt gcgcacccgg tgcatttggc gatcacgaac
     2641 agggctccga acagcgccag cgcgatggca aggaccttcc ggaacgagat caaaacggca
     2701 ccttgatccc cttggacgcc atccagccga tgaggatggc aatggcgtac cttccgatcc
     2761 gcttgacggt ggggtttctc gaaagcgtct tgatgccact ctccacggac gtgagcagcc
     2821 ccagggccgt gtctaccttt gtcgcgagcg cctcgcgcgc cgccttctcg gcatccagct
     2881 ccgccttggt ctgagcaagc ctcgactcgt gcgtgaggtc tacctcagac gcggacgccg
     2941 cccggtggct gttgtttttc aaccgctgat cgacgtcccc cttccactcg aagatgagcg
     3001 cgacctcttt cttgacgagc cccaactcgc cgccctggag gtcctggttc gcctcgaggc
     3061 gatctacctt ctcgtcaagg ttgcggaagc ctatggcaac cttgttgtcc agggcggtca
     3121 ggccctgggt catcagagtc ttgatctccg ccagcaagat ttcgttcttg tcggcggcgg
     3181 ccagctgggc cgtcttgggt tgcgggggca tgtcgtctac catggtgccg ctcctcacaa
     3241 cgccagcgcg taacgcgagt tgacgtagct cttgatgttg tcgaaattgg catcggagat
     3301 gacgccaagg gagaggccga tctcgagaat cgagcccgtg aagctctttg tgccgtcgta
     3361 ggcctgcgag acgaggaacg gcgcggtcag gtcgcccagc gtgcctgccg ccgttgagga
     3421 gtaagagccg ccgtttgtgc ggatcttgat gttcgttccg tcgtacttcc actgaacgag
     3481 cgtccacgca cccgttgcaa tggcagtgga gacgccttgc tgcgccccat caaagtggaa
     3541 cagcgttgcg ctcttggtcc ccgtgctgtc cctaaggacc aggccggcgt aggggccatt
     3601 gctccaaacg ttattgttgt cgtagaggct tggggctgcg atgtccgtgc tgatagacgt
     3661 caccttcatg aggacccacc ccgagtacgc ggtgtccgtt gcgtaggtgg agaatgcgcc
     3721 ggcagtaccg aggaactgcg tcgagccgtt gaaggccgct ggcgtcagtc cgttgaccgc
     3781 ggttccggag gtggggtaat cggtagcctc gtccaggggg tcgttccctc cagacgtgcc
     3841 ggcgctggcg ttctgacccc acggaggggt cacgtagttg gcgcgccacc acgccgagag
     3901 gaccagcgta gccgggtcaa agaccggcgc gccgaccggg cggaccatgc gggccagcat
     3961 taggggatca ccgtcagtcg cgtgaggatg ctggtccact gaatggtcgt ggcggccttg
     4021 cccgtgattc gaagtcgaat cgtggtggtg gaattgtcaa tcgtcgcgtc ccatacggaa
     4081 tccgactccc cgataatcgt cgtggtggat gtgccaacct gcgcggccgt gcccgcagca
     4141 ttccgaaagc acgcggtgac cgaataggcg gcagcctgag acgagtcgga cttgatggcc
     4201 gtcaccagcc acgagacaac caccgcggag ttgctcgcga tggtgaagct gtcgagtgtc
     4261 gtgacggtcg cgtccgttgt ctgaacgttg acggggttga cgtcgacagc cgtccccttg
     4321 gcattgttgc tcgtgcttgc tgagttgcca ctgaggacgg gcaggccgct gagcgtcggg
     4381 gcgtcaatgg ttggcgaggt cgcgaacacg agggcgccac ccgagcccgt ctcgtcggtg
     4441 caggctgtct tgatgttcgc gctagagaac gtccccagcg ccgtggccac gccggaccca
     4501 agggcggtaa ggccggttcc tcccttcgtc tcggggagcg ccgtggtaag cgccgaggcc
     4561 aggttggccc cgctgggcgt tcccaaccat gtcgccacgc cggtgccgag cgacgttagc
     4621 cctgtaccgc cctttgtgtc cgggagcgcc gtggtgaggg cagacgccag gtttgccccg
     4681 cttggggttc ccagccacgt cgccacgccc gtaccaaggg ccgtcaaccc tgtgcctccc
     4741 tttgtgtcgg gaagcgccgt ggtcagggcg gaagcgaggt tcgcgccgct tggcgttgcc
     4801 aggaacgtcg cgacaccagt gccgagtccg gtgacgtcgt tggcgatacg gacgctcagc
     4861 g
//
LOCUS       DASRJQ010000279         4356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1584438, whole genome shotgun sequence.
ACCESSION   DASRJQ010000279 DASRJQ010000000
VERSION     DASRJQ010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1584438"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2318)
                     /locus_tag="VFB89_06300"
     CDS             complement(<1..2318)
                     /locus_tag="VFB89_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM26947.1"
                     /translation="MRSAPDDRLLRLAEDLADGKGAHWDEVLRQEPDLAPALERFRQL
                     QAIAAAHETGARGQREDPWRVPNESLKIPLFSWGEIQVLEKLGEGGYAVVFRAWDPAL
                     EREVALKLNRSDAGLSATELQRWVDEARRLARVRHSNILVIHGVDVRDGRAGFWTDLV
                     HGQTLEQVLQDQGRLGGAEALLVGLDLCRALAAVHAAGLVHGDLKSSNVMREGGAGEA
                     AGTGAGRIVLTDFGASTESSPSVSRSAAFATPLTSAPEVLRGEPPSPASDLYSLGVLL
                     YRLVTGRYPIESTDPTELETKIQYGLRTPLRDLRADLGAAFVAVVERALDPDPKRRYS
                     SAGEMERSLGEALSEESHSAKESVGAPARAATIEEGAVISPFGQRWRASLAVTVIAIV
                     IASLATVWWVGRRRPSGPGPIRFTIGAPPHKLIRTDPNQFAISPDGSTLAFIAYDSTG
                     QSLWVRRLGNHKPRELAGTPGAASPFWSPDGKQIGFFSEAKMRRVGLDGEAPEVICDA
                     PHPHGASWGRKDVIVFAPGNGSLFRVPASGGTPESVTTIDTTGGVNAHAWPGFLPDGE
                     HFTYVARRIEPFSIDDAPRPGPWTSYVGSIRTKSSAPLLTANSVAMYAPPHYVIYIRD
                     GILFAQRFDVRQLRVEGRPIRIADSPAPLSYVGSRCGSVSANGVLVWRSVGSLKTRLV
                     WIDRSARPIREPGIPADRWVRALISPDGRRVILSKETPTDASLWLFDFERGTLTGLTS
                     VPERLELGVWSPDSKEILYRRQGPDAVSDVHR"
     gene            complement(2302..2874)
                     /locus_tag="VFB89_06305"
     CDS             complement(2302..2874)
                     /locus_tag="VFB89_06305"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZM26948.1"
                     /translation="MAADLETTSELLALIRQGDLAARDVLMRRYLPILTGWARGRLPS
                     YARDLAQTDDLVQDTLRAALNHLDEFELRREGAFLAYLRRSLLNRVRDEIRRVRRGPL
                     REAMDDGVADKRRSVVEEAIGRETLERYEAALGELSEEQREAVILRLEFGYTYPQIAA
                     AMGKPTANAARMVVARALVRIVEAMNAFRP"
     gene            complement(3245..4027)
                     /locus_tag="VFB89_06310"
     CDS             complement(3245..4027)
                     /locus_tag="VFB89_06310"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZM26949.1"
                     /translation="MTHPLPIRFQRYVAIGDSSTEGLDDPDGHGGYRGWSQRLAERLA
                     EAQGGLLYANLAIRGRTTRQIRDLQLGPALAMRPDLATVFSGTNDVIARTFNLASVAA
                     DMEHIQRALVDGGATVLTFTLPDLTPIMPLARSLAPRIVAMNEALRSAASRTGAILLD
                     FAAYPVATDARLWSKDRIHANALGHERIAAALAHALGLPGTDEAWKEPLPAAAPRSRA
                     QRWGAELRWTSRYLGPWLARGLFRSVVQSPRQPKRPELTPIT"
     gene            4166..>4356
                     /locus_tag="VFB89_06315"
     CDS             4166..>4356
                     /locus_tag="VFB89_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26950.1"
                     /translation="MLNARVARICALLVFGLASMPAPSGARPFDVPGFGISVPIVRIS
                     GGPVTGTSDPLIAELAQSEA"
BASE COUNT          715 a         1455 c         1480 g          706 t
ORIGIN      
        1 atccgatgga cgtcggagac ggcgtccggc ccctggcgcc ggtagaggat ctccttgctg
       61 tcgggggacc acactccaag ctcgaggcgt tccggcacgg aggtgagtcc ggtcagggtt
      121 ccgcgctcga aatcgaacag ccaaagactg gcgtcggtgg gcgtctcctt gctcaagatc
      181 acccggcggc catccggaga gatgagtgcg cggacccagc gatcggcagg gatcccgggt
      241 tcccggatcg ggcgcgcgct acgatcgatc cacaccagtc tggtcttcag gctgcccaca
      301 ctccgccaga ccaacacgcc gttggccgag acgctgccgc atcgcgagcc aacgtaggag
      361 aggggagccg gcgagtcggc gatccgaatc gggcgtccct ccacccgcag ctgccgcacg
      421 tcgaagcgct gcgcaaagag gatcccgtcc cggatgtaga tgacatagtg gggaggggca
      481 tacatggcga cgctgttggc ggtcaggagg ggggccgagc tcttggtgcg aatcgacccc
      541 acgtaggaag tccaggggcc gggccggggc gcatcgtcga tcgagaacgg ctcgatccgt
      601 cgggcgacgt aggtgaagtg ctcgccgtcg gggagaaagc ccggccacgc gtgtgcgttc
      661 acgccgccgg tcgtatcgat cgtcgtgacg gattcaggtg tgccgccact cgcaggcact
      721 cggaaaagcg acccgtttcc aggcgcgaag acgatgacat ccttgcgccc ccagctcgcg
      781 ccatgcggat ggggcgcgtc gcagatcacc tcgggagcct caccgtccag ccccacccgc
      841 ctcatcttcg cctcgctgaa gaatccgatc tgcttcccat cgggagacca gaaaggagaa
      901 gcggcacctg gcgtgccggc caactcgcgg ggtttgtggt ttccgagccg gcgcacccac
      961 agactctgac ccgtcgaatc gtaggcgatg aaggccagcg tgctgccgtc cggagagatg
     1021 gcgaattggt tgggatcggt cctgatgagc ttgtggggcg gggccccgat cgtgaaccgg
     1081 atcggtccgg gtccggaggg cctgcgccgc ccgacccacc acacggtcgc cagcgacgcg
     1141 atcacgatcg cgatcacggt cactgctaga gacgctctcc agcgctgccc gaaaggtgag
     1201 atgacggcgc cttcctcgat ggtggccgca cgggccggag cgccgacgga ctctttcgca
     1261 gagtggcttt cctcggacaa ggcttcgccg agcgagcgct ccatctctcc ggcgctcgag
     1321 tagcgtcgct tcggatccgg atcgagcgcg cgctccacga ccgccacgaa cgctgcgccg
     1381 aggtcggcgc gcaggtcacg caatggagtc cggagcccgt attggatctt ggtttcgagc
     1441 tcggtggggt cggtggactc gatcgggtag cgcccggtaa ccagtcggta gagcagcacg
     1501 ccgagggagt agaggtcgct cgcgggagaa ggaggctctc cccgcagcac ctcgggggcg
     1561 ctggtgagcg gggtggcgaa ggcggcggat cgcgagaccg acggagagga ttcggtggac
     1621 gctccgaagt cggtgagcac gatccgcccc gctccggtgc cggccgcttc cccggcaccg
     1681 ccctcgcgca tcacgttcga ggacttgagg tcgccgtgaa cgagacccgc ggcgtgcacc
     1741 gcggccagtg cccggcagag atcgaggccc acgagcagcg cttcagcccc gcccagccga
     1801 ccctggtcct ggagcacctg ctcgagggtc tggccatgga ccaggtcggt ccagaagccg
     1861 gcccggccgt ctcgcacgtc cactccatga atcaccagga tgttggagtg ccgaacgcgc
     1921 gccagcctgc gtgcttcgtc gacccatcgc tggagctcgg tcgcgctcag gccggcgtcg
     1981 gagcggttca gcttgagcgc cacctcgcgc tcgagggccg ggtcccaggc gcggaagacc
     2041 acggcgtagc ccccttcccc gagcttctcc aggacctgga tctcgcccca tgaaaagagg
     2101 gggatcttca gagactcatt tggaacccgc cacggatctt cgcgctgccc gcgggcgccg
     2161 gtttcgtgcg ctgccgcgat ggcctggagt tggcggaatc gctcgagggc cggggcgagg
     2221 tccggctcct ggcgcagcac ctcgtcccag tgggccccct ttccgtccgc cagatcttcc
     2281 gccaggcgga gcagacgatc atcaggggcg gaacgcattc atggcctcga cgatccgcac
     2341 cagggcacga gccaccacca tccgagcggc gttggccgtc ggttttccca tcgccgcagc
     2401 gatctgcgga tatgtgtatc caaactcgag ccggaggatc acggcctccc gctgctcctc
     2461 cgagagttcc ccgagggccg cttcgtagcg ctccaaggtc tcgcggccga tcgcttcttc
     2521 tactacggag cggcgcttgt cagcgacgcc atcatccatg gcttcccgaa gcgggccgcg
     2581 gcgcacgcgg cggatctcgt ccctgacacg attgaggaga gaccgacgca ggtaggccag
     2641 gaacgcgccc tcgcgccgca gctcgaattc gtccaggtgg ttgagcgcgg cccgaagagt
     2701 gtcctgcacg agatcgtcgg tctgagccag gtctcgcgcg tagctcggca gtcttccccg
     2761 ggcccatccc gtgagaatcg gcagataacg ccgcatcagg acgtctcgtg ccgcaagatc
     2821 gccctggcgg ataagcgcga gaagctcact cgtcgtttcc agatcggcag ccattagggg
     2881 ggctctggcg tgtcggatcg tggattgccc tttcgtctta gcgatggggc aacacgcgaa
     2941 cgatgaggtt cggtacaccg aatagctgga accggacccg aggccaagga tcatcgcgct
     3001 tcccaccccg accggcaacg agtctttccc gccctgcggc cgctggttgc cgaaaaccgc
     3061 cggcttccaa tgcccgagtc agatgccgat gtgccgttca cggcatcact tgccaccgat
     3121 ccgccctcga cacgcgaacc gtgccccctg cacttcctaa cgatgaggag gacggaaagc
     3181 gcacgcgttc ggtgcgagcc ggctgcgcgc cacgtacgcg ttcgacagcc gtcggtatcc
     3241 agaatcaggt gatcggcgtc agctcgggtc gcttcggttg tcgtggcgac tggaccacac
     3301 ttcggaacaa gccgcgcgcg agccacggcc ccaggtaccg gctcgtccat ctcagctcgg
     3361 cgccccaacg ctgcgcgcgc gaccgcggcg cggcggccgg cagcggctcc ttccacgcct
     3421 cgtcggtgcc cggcagcccg agggcgtgcg cgagcgctgc ggcgatccgc tcgtgaccga
     3481 gcgcgttggc gtggatgcga tccttgctcc acaggcgcgc atcggtcgcc accgggtagg
     3541 ccgcgaagtc gagcaggatg gcgccggtgc ggctcgccgc cgatcgcagc gcctcgttca
     3601 tcgccacgat gcgcggcgcc agcgagcgtg ccagcggcat gatcggcgtg aggtccggca
     3661 gggtgaaggt caggacggtg gccccgccat ccaccagcgc gcgctggatg tgctccatgt
     3721 cggctgccac cgaggccaga ttgaacgtcc gcgcgatcac gtcgttggtg cccgagaaga
     3781 cggtggccag gtcgggccgc atggccaggg ccggaccgag ttgcagatca cgaatctggc
     3841 gggtggtgcg tccgcgaatc gccaggttgg catagaggag cccgccctgg gcttccgcca
     3901 gccgctcggc cagccgttgc gaccagcctc gatatccacc gtgtccatcg ggatcgtcga
     3961 gtccctcggt ggagctgtcg ccgatcgcaa cgtagcgctg gaaacggatc ggaagtggat
     4021 gggtcacgcg gcgattctaa tggatgcggc gtttacacgt tggaaacatc ggcgtaatat
     4081 tccggttact aagcctcgtt ccgccagcta tttccggcaa cgcgacgggc atcggggtcg
     4141 ggtcttgaga agggcacaca tacatatgtt gaacgctcga gtcgccagga tctgcgcact
     4201 gttggtgttt gggctcgcct cgatgccggc gccgagcggc gcccgcccgt tcgacgttcc
     4261 cggtttcggc atctcggtgc cgatagtgcg gatcagcggc ggcccggtca ccgggacgag
     4321 cgatccgctc atcgccgagc tcgcgcaatc cgaggc
//
LOCUS       DASRJQ010000280         4953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3993555, whole genome shotgun sequence.
ACCESSION   DASRJQ010000280 DASRJQ010000000
VERSION     DASRJQ010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3993555"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1710
                     /locus_tag="VFB89_06320"
     CDS             <1..1710
                     /locus_tag="VFB89_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26951.1"
                     /translation="VDAVREWSARFSAWLLIVAVAWFGIVGLVLLGPEVVRLLVENQG
                     QRAVRIGQIVSGIVGVISGLVTLRAGHSAGTPATQRPAGTPMNLPLLLAAPVFVVFLV
                     GLFGWAGRALIGTVESLATQLPVIGPESFLTAPDWVRPSLIAIAGGVSGALRAVMKPG
                     AVGYIVTSIVVPLILIWFGSWTATRVDTNKFSLHAMYRARLIRAYLGASRPAGERDPN
                     PFTGFDPLDNIWMKDLWPPPGAPFIPPGPRLMHVVNVALNLVGGGNLAWQQRKAESFT
                     FSPLYCGAYNQGYRRTRRPKDDRRRSYGGADGITLGTAMTISGAAVSPNMGYHSSTVI
                     SFLMTLFNVRLGWWLGNPGHAGAKVYEQSSPDSSLRLILDEAQGHTDNKHPYVYLSDG
                     GHFENLGLYEMILRRCHTIVVSDAGCDPSGSFEDLGNAIRKIRVDFGIPIEFQEIKIY
                     PRVPGEPPTAGKYCAIGTIRYSCVDNDADDGTLIYIKPAFYGTEPKDVFNYAQTSSAF
                     PHESTADQFFSESQFESYRALGSHAIDQILGSQPTGVEPQFTPIGRLVRRAGDYVRQG
                     GSA"
     gene            complement(1724..>4953)
                     /locus_tag="VFB89_06325"
     CDS             complement(1724..>4953)
                     /locus_tag="VFB89_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26952.1"
                     /translation="PSRVLREIRPGVMLFRRTSANRRRLVVLYVVPELPTRGVYKEAL
                     RAALEDRRALLEHVDLPVRPDRTEPIRIIGPTFSGAALSLQFALRGWLASRPTDSVEI
                     VSGSATSATNLETLNRPDLRMHFSATINPDVSLTQAFSNVVLPRLGLRPSQVALLRES
                     STQYGQDLLDTDSASRSTADTGQFVIIPFPMSISSLRAEYQRVPMGGAAPAGPPLPGA
                     EAPRLPLDLLDPTRPKEDLPVTSRLSPLALDLILDDLARTLVRRQIRLVGLLATDVRD
                     KLFLGDEIRKRVRDVQFFTYQSNILYLRSDRSLALRGMLVLSTYPLILENQWSTSSDR
                     SGQRFAFGSDGAEGVYNATLLQLGTNRALVDYRRTRDTVLYRPPVWLSTVGNQTFLPV
                     TVMVDSLDTAYVAPSCRRGEGCPKPGEWPAVRLPFLPLATMLLTSFALLLLSLSNLID
                     DRKVRALLKTRGVTEEFEHRPLAEQVMVGSLKLHGRLYRVLRIVAAIGVFLSAWAPVG
                     LLIWRGLGRPGSSIYLVLFGIGTGATVLSLAALASCGFTMLEIGRRIARPGWQFFQAG
                     PPWTEPGEKWSWRTEVVARTAVAVFGIIYLVLVVWFTIQVLTLHGGSYWLFFRRAVEV
                     DSLVSPVLPLILGGIGYFVWCTWHIERIALLQNYTTFEGACEAEFGSQWVPRPTVRSA
                     LREDFRRSGGIARAIRSRLFQVIPSPAAFGLLAAFVCLAWWLAPQFGRSLEAILIGSR
                     GAPLTAFDVLFRVTVIASMFATAWGALRLVVVWAGLRQCLTGFGRMPLVTAFDRLPPR
                     LSRLTRLTLPGAAPRTGGAVADLQWLHLQRIHAATRVEFGTKLSELDASLPPRVERLM
                     AETPTQASTLDWRGRTDLIARLIALHELLRELWRLEPMPDDVTALTQSLLKEFDRPDP
                     TGAPAVSTTVRVRRGFAGPVRLWLRAAEEYVASRMVEYIEWVIRHLRVLALFMLLSLV
                     LTTLLVSSYPYHPQSLLRLILLLVMVFTVGAFIAVLVQMNRNEVLSRIAHTEPGEVTW
                     NASFILNLFTFGVVPLLTLLSSEFPELRNALFAWVQPLVNALVKQ"
BASE COUNT          955 a         1584 c         1597 g          817 t
ORIGIN      
        1 gtcgatgcgg ttcgcgaatg gtccgctcgc ttcagtgcct ggctgctcat cgtcgccgtg
       61 gcctggttcg gtatcgtcgg gctggtgctg ttgggacccg aggtcgtccg cctgctggtc
      121 gagaatcaag gtcagcgggc cgttcgcatc gggcagatag tgagcggcat tgtcggtgtg
      181 atctccgggc tcgtgaccct gcgcgcggga cacagtgccg ggaccccggc gacccagcga
      241 cccgccggca ccccaatgaa tctgcccctc ctgctcgcgg cccctgtttt cgtcgtgttt
      301 ctggtggggc tgttcgggtg ggcaggccga gcgctgattg gcacggtcga aagcctggcg
      361 actcaacttc cggtcatcgg tccggaatcc ttcctcaccg ctccggactg ggtccgccct
      421 tctctgattg cgattgcggg cggggtgagc ggtgccttac gggccgtgat gaaacccggc
      481 gccgtcggct acatagtgac cagcatcgtg gtaccgctga ttctgatctg gttcggctcg
      541 tggaccgcga cccgggtaga caccaacaag ttctccctgc acgcgatgta tcgggcgcgg
      601 ttgattcgcg cttacctcgg cgcttcccgg cctgccggcg agcgcgatcc gaacccgttt
      661 accggcttcg atccgctgga caacatctgg atgaaggacc tctggccgcc accgggggcg
      721 ccctttattc cacccggtcc ccgcctgatg cacgtggtga acgtggcgct caatctcgtg
      781 ggcggcggca acctcgcctg gcagcagcgt aaggcggaat cgttcacgtt cagcccgctc
      841 tattgcggtg cttacaatca gggctaccgc cgcacccggc ggccaaagga cgatcggaga
      901 cgttcgtacg gaggagctga cgggatcacg ttgggcaccg cgatgaccat ctccggtgcg
      961 gccgttagcc cgaacatggg gtatcactcg tcgaccgtga tctcatttct gatgacgctg
     1021 ttcaacgtcc ggcttggatg gtggttgggc aatcccggtc acgccggagc gaaggtgtac
     1081 gagcaatcat cgcccgactc gtcgctccgg ctgatcctcg atgaggcgca ggggcacacg
     1141 gacaacaagc atccgtacgt ttacctgtcc gacggcggtc acttcgagaa tctgggactg
     1201 tacgagatga ttttgcgacg ctgccacacg atcgtcgtga gcgacgccgg ctgtgacccg
     1261 agcgggagct tcgaggatct gggcaacgcc attcggaaga tccgggtgga cttcggcatt
     1321 ccgatcgagt ttcaggagat caagatctat ccccgggtcc ccggcgagcc ccccacggca
     1381 ggcaaatatt gcgcaatcgg caccattcgc tattcctgcg tcgataatga cgcggacgac
     1441 ggtacgctga tctacatcaa gccggccttc tacggcaccg agccaaagga cgtcttcaac
     1501 tatgcgcaga ccagcagcgc attcccccat gagagcacgg ccgatcagtt cttcagcgag
     1561 tctcaattcg agagctaccg cgcgctcggg tcacacgcca tagaccagat cctgggaagt
     1621 cagccgacgg gggtcgagcc gcagttcact ccgatcggcc gtctggtccg ccgggcgggc
     1681 gactacgtcc gtcagggtgg ctccgcctga gcgtcttgtc ggcttactgc tttaccagcg
     1741 cgttcaccag cggctgcacc caggcaaaga gagcattgcg cagctccggg aactccgagc
     1801 tgagcagcgt gagcaggggg accacaccaa aggtgaagag attgaggatg aagctcgcgt
     1861 tccaggtgac ttcgccgggc tcagtatgcg cgatccgact gagcacctcg ttccgattca
     1921 tctggaccag cacagcaatg aaagcaccca cggtgaagac catcaccagg aggagaatga
     1981 ggcgcagcag actctgaggg tgatatggat agctggagac cagcagggtc gtgagtacca
     2041 gggacaagag catgaacagc gccagtactc ggaggtgccg gatgacccac tcgatgtact
     2101 cgaccatccg actcgccacg tattcctccg ccgccctcag ccagagccgc acgggacctg
     2161 caaacccccg ccggacccgc actgtggtcg ataccgcggg ggcaccggtt ggatccggcc
     2221 ggtcgaactc tttgagcagg ctctgagtca aagcagtcac gtcgtccggc atcggctcga
     2281 ggcgccatag ctcccgcagc agctcgtgca aggcgatgag ccgggcgatc agatcagtgc
     2341 gcccgcgcca gtcgagtgtg gatgcctggg tgggggtttc ggccatcagc ctctcgaccc
     2401 gtggcggcag cgacgcatcc agctcgctca gcttcgtacc gaattccacc cgcgtagcgg
     2461 cgtggatgcg ctggaggtgc agccactgca agtcggcaac agcgcctccg gtcctcggcg
     2521 ccgcaccggg cagggtcaga cgggtaaggc gcgacaagcg cggcggcagg cgatcgaacg
     2581 cagtcacgag cggcatcctg ccgaagcctg tgagacattg gcgtaaacca gcccacacga
     2641 cgaccaagcg aagtgcaccc cacgccgtgg caaacatcga agcgatcaca gtcacgcgaa
     2701 acagcacgtc gaacgcggtg aggggagctc cgcgggaccc gatgagaatg gcctcgagcg
     2761 aacggccgaa ctgaggcgcg agccaccagg ccaagcagac aaaagctgcg agaagtccga
     2821 aggcagccgg ggagggaatg acctggaaaa gccggcttcg aatcgcccgg gcgatgcccc
     2881 ccgatcgccg gaaatcctcc cgcaatgccg aacgaacggt gggacgaggt acccattgac
     2941 tgccgaactc agcttcgcag gcgccctcga aggtggtgta gttctgcagc agcgcgatgc
     3001 gctcgatgtg ccaggtgcac cacacgaaat acccgatgcc gccaaggatg agcgggagga
     3061 cgggcgacac caggctgtcg acctccaccg cccgccggaa gaacaaccag taggagccgc
     3121 cgtgcaacgt gagtacttgg atggtgaacc agaccaccag gacgaggtag atgatgccga
     3181 acaccgcgac agccgtgcgg gccacgacct ccgttctcca gctccatttc tctccgggct
     3241 cggtccacgg cgggcccgcc tggaagaatt gccagccggg ccgggcgatt cggcgcccga
     3301 tctccaacat cgtgaatcca cagctcgcga gcgcagccag gctcagcact gtcgccccgg
     3361 tcccgatgcc aaacagcacc agatagatgc tgcttccggg acggccgagt cctcgccaga
     3421 tcagcagacc taccggcgcc caggcggaaa ggaaaacgcc gatcgctgcc acgattctga
     3481 gcacacggta cagccggccg tgcagcttga gcgaaccgac cattacctgc tcggccagcg
     3541 ggcggtgttc gaactcctcg gttacgcccc gagtcttgag cagcgcgcgg accttgcggt
     3601 catcgataag gttggaaagc gacagcagca agagcgcgaa gctcgtgagc agcatggtcg
     3661 cgagaggcag gaagggcagc cggaccgcgg gccactcgcc cggcttggga cacccctctc
     3721 ctcggcgaca gctgggcgcg acataggccg tgtccagtga atccaccatc acggtgactg
     3781 gaaggaaggt ctggttcccg accgtggaca gccataccgg cggtcggtac agcacggtat
     3841 cgcgggtgcg ccggtagtcc accagcgccc ggttcgttcc cagttgcagg agcgtcgcgt
     3901 tgtacacccc ttccgcccca tcgcttccga acgcgaaccg ctgacccgaa cgatcgctcg
     3961 acgtgctcca ctgattttcc aggatcagcg ggtaggtgga cagcaccagc atacctctga
     4021 gcgccaggct acggtcggat cggagataga ggatgttgct ctggtaggtg aagaactgga
     4081 cgtctcgaac ccgtttcctg atctcgtcgc ccaggaacag cttgtcgcga acatcggtgg
     4141 cgagcagccc caccagcctg atctgtcgcc gcaccagcgt tcgggccaga tcgtccagga
     4201 tcaagtcgag cgcgagcggc gagagccggg atgtgacggg caaatcctcc ttgggccgcg
     4261 tagggtcgag cagatcgagc ggcaaccggg gagcctcggc ccctggaagc ggaggaccgg
     4321 caggcgcggc acctcccatc ggcacgcgct ggtactcggc ccgaagactc gagatgctca
     4381 tcgggaacgg gatgatcacg aactgtccgg tgtccgccgt cgagcgggag gcgctgtcgg
     4441 tgtcgagcaa atcctggccg tactgggtgg acgattcacg aagcagggcc acctgcgacg
     4501 gtcgaagccc cagtctcggc aacaccacgt tggagaacgc ctgtgtcagg ctgacgtccg
     4561 ggttgatcgt cgcgctgaaa tgcatcctga gatcgggccg attcagcgtc tcgagattgg
     4621 tggcgcttgt cgcggatcca ctcacgattt ccacggaatc ggtcggacgg ctcgccagcc
     4681 agccacgcag cgcgaactgc agcgacaagg cggccccaga aaacgtcggc ccgataatgc
     4741 ggatcggctc ggtccgatcg ggacggactg gaaggtcgac gtgctcgagg agggcgcgcc
     4801 tatcctccag cgcagcgcgg agcgcctcct tgtacacccc tcgggtggga agctccggta
     4861 ccacgtacag cacgaccagc cgccgccggt tggctgacgt gcgccggaac agcatcacgc
     4921 cgggccggat ctcgcgcagg acgcgtgaag gca
//
LOCUS       DASRJQ010000281         2561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3993185, whole genome shotgun sequence.
ACCESSION   DASRJQ010000281 DASRJQ010000000
VERSION     DASRJQ010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3993185"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VFB89_06330"
     CDS             <1..760
                     /locus_tag="VFB89_06330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HZM26953.1"
                     /translation="GYELVHHGEGQWNGVAIAARGGVADVVTNFGEPLQRARTSSVGD
                     DEPLAEARMIAATYSGVRVVSLYGPNGRSVGSPFYQAKLAWYARLSQWLETMADPAEP
                     LVLGGDFNVAPEDKDVWDPVACHGGTHVSQPERDAFFRLLHWGLVDAYRLHRAEPGRY
                     TWWDYRAGNFHKNFGMRIDHLLVTRPVAERTIWAEIDREARKGKPIPSDHAPLVIDLD
                     RPGHPLDAGWAAAEARIASRGTRLAPRSSSEQPS"
     gene            808..1389
                     /locus_tag="VFB89_06335"
     CDS             808..1389
                     /locus_tag="VFB89_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HZM26954.1"
                     /translation="MASSRTRGASAALMGGSYRQCTECGKRALSIATRCPGCGRELNA
                     PIAQAAPAVGSSLDLRRFLSPGLVGGLIAATVFLAAALMARPSGPIESRSSLVAADST
                     STFSEVAYTTAAAARLDTATAAPLPPAREGEVLVARTWTHVRHSRSKDADVDAVLLPG
                     DTVFADSLGRGWYRVALDGEVLGYVHRSTLTHP"
     gene            complement(1711..2013)
                     /locus_tag="VFB89_06340"
     CDS             complement(1711..2013)
                     /locus_tag="VFB89_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26955.1"
                     /translation="MRAELIEIVETELKKFGAELKLSDAQKSQLKTALENAHERIDEF
                     RAKNPNISKADVVAKLKEVRGSIRERLEKFLTPEQLTKWDSEVTKAKKFLGHSVES"
BASE COUNT          428 a          835 c          807 g          491 t
ORIGIN      
        1 cgggtatgag ctggtccatc atggcgaggg ccaatggaac ggagtcgcca tcgccgcccg
       61 cggcggtgtc gcggacgtgg tgaccaattt cggcgaacct ctccagcgtg cgcggacgtc
      121 gagcgtcggt gatgatgagc cgctggccga ggcgaggatg atcgcagcca cctacagtgg
      181 tgtgcgggtc gtgtccttgt acggaccgaa cggacggtcg gtcggctcac ccttctacca
      241 ggccaagctg gcctggtacg ctcgtctctc ccaatggctc gaaaccatgg ccgatcccgc
      301 cgagcccctg gtgctcgggg gtgacttcaa tgtcgctccg gaggacaagg acgtgtggga
      361 tccggtcgcc tgccacggcg ggacccacgt ttcccagccg gagcgggatg cctttttccg
      421 gcttttgcac tggggtctgg tggatgccta tcgcctgcat cgtgccgaac cgggtcgcta
      481 cacctggtgg gattaccgcg ctggaaactt ccacaagaac ttcggcatgc gaatcgatca
      541 cctgttggtg acgcgcccgg tggcggagcg caccatctgg gccgagatcg atcgggaagc
      601 gcggaagggc aagccgatcc catcggacca tgcgccgcta gtgatcgatc tcgatcggcc
      661 cggccacccc ctggacgccg gttgggctgc cgcggaggcg cgcatcgcgt cacgtggcac
      721 ccgacttgcc ccccggagct cgagcgagca gccctcatag tccaaccgcg aagcgatggt
      781 ggcttcgggt caggccttac gggtccgatg gcgtcgagcc gtaccagggg agccagcgca
      841 gcgctgatgg gtgggtccta cagacagtgc accgaatgcg gcaaacgggc cctgagcatc
      901 gccacccgtt gccccggctg cggccgcgag ctgaatgccc cgatagccca ggcggcgcca
      961 gcggttggct cctccctgga tctgcgccga ttcctgtcac ccgggttggt aggcgggcta
     1021 atcgctgcga cggtcttcct ggcggcggcg ctgatggctc gacccagcgg acccattgaa
     1081 tcccgttctt ccctcgtcgc ggccgactcc acctcgacct tctccgaggt cgcctacacc
     1141 accgctgcgg cagcccggct ggacacagcg acagccgccc ctctaccgcc tgcgcgcgaa
     1201 ggcgaggtgc tcgtcgctcg aacctggacc cacgtgcgtc attcgcggag caaggacgcg
     1261 gacgtcgacg cagtgctcct gcccggcgac acggtgttcg cggactcgct cgggcggggc
     1321 tggtatcggg tggcgttgga cggagaggtg ctcggctatg ttcaccgctc gacgctcacg
     1381 catccctagg tttggcgacc tggtgggcat cagcaatcgc cgaccttcgt tgctggtcgg
     1441 gctgccgaac gccgccgcgc ttgtcgcctt agtgcagacc gccgacacgt tgctgcgtca
     1501 cttcgagacc ggggaaacgc cttcaatccc accaacctgc aactactcac gcagtcctgg
     1561 taaagccggc cacgtacaag tttggcagcc cgcgaattca agacccacga agcctatcgt
     1621 cctattgtcc tgcaggcagc ttccctccgc acataagcac gtggcttatg tgcggagggc
     1681 ggcaacgccc ggcccgtcga tcaacccttt ctaggattcc acggagtggc ccaggaactt
     1741 cttcgccttg gtcacttcac tgtcccactt cgtgagttgc tccggggtaa ggaacttttc
     1801 cagtcgctcg cgaattgagc cgcgaacctc cttgagcttc gcgacgacgt cggctttcga
     1861 gatattgggg ttcttcgcac gaaattcatc gatcctctcg tgcgcattct ccaacgccgt
     1921 cttcagctgg ctcttctgcg catccgatag cttcagctcc gcgccaaact tcttcaactc
     1981 ggtctccacg atctcgatca gttctgctct catgattcag tctcctttga gtgccgctcc
     2041 gagctcagcg gaaaggcgga tgatcgttgt gtctctcgcg agtgcacctg aattgctttg
     2101 ccgtgctcgg ccccccgggg ccgagctcaa acgggtgtaa agacgttatg ggtcacgatg
     2161 atcgctgccc gcggcatggt ttccgcttga tcagccttgt ctccgttgcg tcgccctcag
     2221 tgccgcgctg tgtgcgaggc ggctgtgcca gccgtaggca gcgacggggc gttctcgaga
     2281 gggtctagag taggaagtgg gacgcgggac ggaaactggc agttttgaca ggcccggcag
     2341 cggtattcga agtccatccc gagtggaacg gcattgccaa tcggggatca cagcaccggt
     2401 gatgccaaag actagactcc agcgatcgtc agcacgccga cggctaagat cgccacgcct
     2461 acaacccgcg cgactcgcag cgggactggc gtcaaccgct cggcggagat cgcgagggtc
     2521 acagcagcca ttgcgaacag gtgcatcata ccggtggcga g
//
LOCUS       DASRJQ010000282         2532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_914750, whole genome shotgun sequence.
ACCESSION   DASRJQ010000282 DASRJQ010000000
VERSION     DASRJQ010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914750"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VFB89_06345"
     CDS             <1..222
                     /locus_tag="VFB89_06345"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HZM26956.1"
                     /translation="VFSAFNRLGRIMQRLTAITVIIMVPNFVASIYGMNFIHLLPPSD
                     SAYGFLAVGAFLMVMVAWGFIHSKMLGWL"
     gene            368..751
                     /locus_tag="VFB89_06350"
     CDS             368..751
                     /locus_tag="VFB89_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26957.1"
                     /translation="MPVTYEINTTRRLIHTHCVGPVTLEEVLGHFTELIQDPACPDHL
                     DVLLDLTETTSLPTSNQLRAVSSEIARVSPRVQFGRCAIVTSNDAWFGTARMFEVYAV
                     NYFRATDVFRTVNDAVLWLESPNVA"
     gene            741..2189
                     /locus_tag="VFB89_06355"
     CDS             741..2189
                     /locus_tag="VFB89_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZM26958.1"
                     /translation="MSHELAAERRRLSQAISGSGFFALGFGTIIGVGWIIVLGDWLRQ
                     AGPLGAILAFLAGGLVMTVVGLCYAELATFLPVSGGELAYAYEAYGVKACFATGWALA
                     LVYIAFTAFEAVSLAWVLGTLLPGIQGPTLYVSRGEPVGLGSLLLGLGGMGLFTGLNY
                     RGARSAARTQTILTYTKIGVSALFITAGIAGGAVVNLQPLFAERTGWRGILAVFLTTP
                     VWFGGFNVIPQAMEERSPGTSLRTVGRVIILSLGLATAFYCLIILASSMTTPWQGLLT
                     MDLPAARAFEVGLRSTLLAKVVLLAALLGLIATWNAVFFAAARVLFALGRARIIHPSF
                     GKIHPAYGSPANAVLFVGALGTCAVFIGRSAIIPIVNVGATCLAGAFLLTCLGVIRMR
                     RLKPDQPRPYAVPGGSWTARIAVFFCLGILALSLYQPYADTRGTVPLEWIFLGLWIMM
                     GSVFWRFAHRGRDAVSTQERRRLILGDAAGVP"
     gene            2186..>2532
                     /locus_tag="VFB89_06360"
     CDS             2186..>2532
                     /locus_tag="VFB89_06360"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HZM26959.1"
                     /translation="MSSAAPGATHIATVAVVGTGVIGRSWAQVFARAGCNTRLYDPDH
                     AQVQRARDAIARSVDYDVAEGFETPVSAVACMERLSLHADLRAALSGAGYVQESGPED
                     FGLKRAIHAELDRL"
BASE COUNT          391 a          808 c          807 g          526 t
ORIGIN      
        1 gtgttctccg cgttcaaccg gctgggccgc atcatgcaac gcctaaccgc catcaccgtc
       61 atcatcatgg tgcccaactt cgtggcgagc atctatggca tgaacttcat ccatctcctg
      121 ccgcccagcg actcggccta cggctttctc gcagtcggcg cgtttctcat ggtgatggtg
      181 gcatggggct tcatccattc gaagatgctg gggtggcttt aagagcgggg aatcgggctt
      241 tggggggaaa gggcgccagt atcaggaagc cgctccgtgc cggcacgccc accatgtgct
      301 ctggcgccag cgacaggccg tcgtagcttt gctggttgcc aagttgcggc tcgaaagaca
      361 ggactcgatg ccagtcacct acgaaatcaa taccacgagg cgcttgatcc atactcactg
      421 cgtcggacct gtcacgctcg aggaggtgct ggggcacttt accgagctga tccaggatcc
      481 agcatgcccg gatcacctcg acgtcctgct cgacctgacc gaaacgacct cgttgcccac
      541 gagcaaccag cttcgcgcgg tgtcgtccga gatcgcccga gtcagccctc gagtccagtt
      601 cggccggtgc gccatagtca cttcgaacga cgcgtggttc ggaaccgcac ggatgttcga
      661 ggtatacgca gtcaattact tccgcgcgac ggacgtcttt cgtacggtga acgacgccgt
      721 gctctggctc gaatccccga atgtcgcatg agctagctgc ggagcggcgg cggctcagcc
      781 aagcaatcag cggtagcgga tttttcgccc tcggcttcgg cacgatcatc ggcgtggggt
      841 ggatcatcgt gctgggcgac tggctccgac aggccggccc cttgggagcg atcctcgcct
      901 tcctagcagg cggcctggtg atgacggtcg tgggtctctg ctatgcggag ctggcaactt
      961 tcctgcctgt ctccggtggc gagctggcgt acgcgtacga ggcctatggc gtcaaagcct
     1021 gctttgccac gggttgggcg ctcgccctcg tttatatcgc gttcaccgca ttcgaggcgg
     1081 tttccctcgc gtgggtactc ggcactctcc tccccggcat ccaggggccg acgctgtatg
     1141 tgagtcgcgg ggagccggtc ggattgggca gcttgctgct gggactcggg ggaatgggat
     1201 tgttcaccgg tctcaactat cgcggcgcca gatccgccgc ccgtactcag acgattctca
     1261 cctacaccaa gattggcgtc tcggccctct ttatcacagc gggcatcgca gggggagccg
     1321 ttgtcaacct tcaaccgctc tttgccgagc ggaccggatg gcgtggtatc ctggccgtgt
     1381 tcctcacgac accggtgtgg ttcggcgggt tcaacgtgat cccgcaggcc atggaggaaa
     1441 gatctccggg tacgtcgctc cggactgtgg gccgggtgat catcctatcc ctcgggctgg
     1501 cgacggcctt ctactgcctg atcattcttg cgtcctcgat gacgacgccg tggcagggcc
     1561 tcctgacgat ggatcttccc gcggcgcgcg ctttcgaagt cgggcttcgc tccacactgc
     1621 tcgccaaggt ggtgctgctg gcggcgctgc ttgggctcat cgccacctgg aacgcggtct
     1681 tcttcgcggc agcccgagtg cttttcgcgc tggggcgcgc ccggatcatt cacccatcct
     1741 ttggcaagat tcacccagcg tacggctcgc ccgccaatgc agtgctcttc gtgggcgctc
     1801 tcggcacctg cgccgtattc atcgggcgca gcgcgataat cccgatcgtg aacgtggggg
     1861 ccacctgcct tgccggtgct tttctcctca cctgtctcgg ggtgatcagg atgcggcgac
     1921 tgaagcccga ccagccgcgg ccatatgcgg tgccgggtgg cagttggacc gcgcgcatcg
     1981 ccgtgttctt ctgcctggga attctggcac tctccctgta ccagccgtac gcggacacca
     2041 gaggaaccgt gccgctcgaa tggatctttc tcggcttgtg gatcatgatg ggaagcgtgt
     2101 tctggcgctt tgcccatcgc gggcgcgatg ctgtgagcac gcaggagcgt cgtcgcctca
     2161 tcttgggtga cgcggccgga gtgccgtgag cagcgcggca ccgggcgcga cgcacattgc
     2221 cactgtggcc gtcgtcggga caggggttat cgggcggagc tgggcccagg tcttcgctcg
     2281 cgccggctgc aacacccgcc tttacgatcc cgaccacgcg caggtgcagc gcgcgcggga
     2341 cgcgatcgcg cgaagcgtcg actacgacgt tgcggaaggc ttcgaaactc ccgtgtcagc
     2401 cgttgcgtgc atggagcgcc tgtctcttca cgcagacctg agggcagctc tctcgggcgc
     2461 cggctacgtg caggaaagcg gccctgaaga cttcggcctg aaacgagcca tccatgcgga
     2521 gctggatcgt ct
//
LOCUS       DASRJQ010000283         2076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1879336, whole genome shotgun sequence.
ACCESSION   DASRJQ010000283 DASRJQ010000000
VERSION     DASRJQ010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1879336"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..993
                     /locus_tag="VFB89_06365"
     CDS             73..993
                     /locus_tag="VFB89_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HZM26960.1"
                     /translation="MGLLVVGSVALDSVFTPFGETADALGGSAVFFAVAASLLSPVKV
                     VGVVGRDYPWAELERLGQRGIDWSGVERADGESFRWKGKYSYDLQSRETLETRLGVFA
                     DFRPKLPEAFRSSGYLFLGNIDPELQLGVLEQIIRPKLVVCDTMNYWIKGKKEALMAL
                     LARVDILMVNDSEARELSGDWNIHRAGRWILANGPKRVVVKQGEHGAMLIEPGRTFYA
                     PAFPLEEVFDPTGAGDAFAGGFMGYLARTDSISEESMRSAMVYGAAMGSYAVSHFGVR
                     GFEGVTLIDVQRRVRTFRDLTHVALAELVP"
     gene            990..2036
                     /gene="purM"
                     /locus_tag="VFB89_06370"
     CDS             990..2036
                     /gene="purM"
                     /locus_tag="VFB89_06370"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HZM26961.1"
                     /translation="MSDKAGERAGGQYLAAGVDLVAAETAKERIGRLVGATRTSLSMG
                     RIGAFGGMIRVPSDIANPVLVLSTDGVGTKVLVALEAGRFDTVGEDLVNHSVNDILVH
                     AARPIAFMDYVAGSALTVEQTAEIVEGIARGCRTHGMALAGGETAQMPGLYRAGTFDL
                     AGTIVGVVAEEEALHGDRITPGDLLIGYPSSGLHTNGYSLARRVIFEQMKLGVHDRLA
                     GTGQTVADALLAVHRSYARAVAPILSQVHGLAHITGGGIPGNLIRVLPEGCQAVIDPT
                     SWEVPPLFNALQEGGGIDWDEMRDVFNLGVGFIAVLPPASVSAVQRAAAAAGTEAWVM
                     GEIRRGPREVRFAR"
BASE COUNT          344 a          609 c          721 g          402 t
ORIGIN      
        1 gcgcggctcg aaccgtgctc gccaacgcac tcgcgattct tggcatttcc gcccctgacc
       61 ggatgtgagc tgatgggtct cctcgtggtc ggcagcgtcg cactggactc ggtattcacc
      121 cccttcggag agaccgccga cgcactcggt gggtcagcgg ttttcttcgc agtggcggct
      181 tccttgctga gcccggtcaa ggtggttggc gtggtggggc gcgactatcc ctgggccgag
      241 ctggagcgtc tgggccagcg gggcatcgat tggagcggcg tcgaacgcgc cgacggcgag
      301 agcttccgct ggaagggcaa gtactcctac gaccttcaga gtcgggaaac gctggaaact
      361 cgccttggtg tattcgccga tttccggccc aagctgcctg aggctttccg ctcgtccggc
      421 tacttgttcc tcggcaacat cgatcccgag ctgcagctcg gtgtgttgga gcagatcatc
      481 cggcccaagc tggtcgtgtg cgataccatg aattactgga tcaagggcaa gaaggaagcg
      541 ctgatggcgc tgctggcccg ggtcgacatc ctcatggtga acgacagcga ggcgcgcgag
      601 ctctcgggcg attggaacat tcaccgtgcg gggcgctgga ttctcgccaa cgggcctaag
      661 cgcgtggtgg tgaagcaggg ggagcatggc gccatgctca tcgagccggg ccgcacgttc
      721 tacgctcctg catttcccct cgaggaagta ttcgatccca ccggggcggg cgatgcgttc
      781 gccggcgggt tcatgggata tttggctcgg accgactcga tttccgagga gagcatgcgc
      841 tccgcaatgg tctacggcgc agccatgggt tcgtatgcgg tttcgcattt cggcgttcgc
      901 gggttcgagg gagtcacttt gatcgacgtg cagcggcgcg tacggacgtt tcgggacctt
      961 acccacgtcg cgctggcaga gctggttccg tgagcgacaa agcaggtgag agagcgggcg
     1021 gacagtacct cgcagcgggt gtggatctgg tagcggccga gaccgcgaaa gagcgaatcg
     1081 gacggctggt cggtgccacc cgcacctcgc tttcgatggg gcggatcggc gcgttcggtg
     1141 ggatgatccg agttccatcc gacatcgcga atccggtgct ggtgttgagc accgatggtg
     1201 tcggcaccaa agtgctggtc gcactcgagg ccgggcgctt cgacacggtc ggcgaggatc
     1261 tggtcaacca cagcgtgaac gacatcctgg tccatgccgc acgaccgatc gcgttcatgg
     1321 attacgtcgc cggctccgcg ctgacggtgg aacagacggc cgaaattgtc gaggggattg
     1381 cccggggctg ccggacacac ggaatggccc tggccggcgg cgagacggcg caaatgccgg
     1441 gtctgtatcg agccggaacc ttcgatctcg caggcacgat cgtgggagtg gtcgctgagg
     1501 aggaggcact ccacggagat cgaatcacgc caggggatct gttgatagga tacccttcga
     1561 gcggcctcca taccaacggt tattcacttg cccgccgcgt gatcttcgag cagatgaagc
     1621 tgggcgtgca cgatcgactc gcggggacag ggcaaaccgt cgccgatgcg ttgctcgccg
     1681 ttcataggag ttatgctcgc gcggtcgctc cgatcctctc ccaggtccat gggctggcgc
     1741 acatcacagg cggaggcatt ccaggcaatc tgatccgggt gctccccgag ggatgtcagg
     1801 cggtcatcga tccgacttca tgggaagtgc cgcctctctt caacgccctc caggagggcg
     1861 gcgggatcga ttgggatgag atgcgggatg tcttcaacct cggcgtcggc ttcattgctg
     1921 tgctgccgcc cgcctcggta tcagccgtgc agcgtgccgc cgcggccgcg ggaacagaag
     1981 cgtgggtcat gggcgagatc agacgggggc cgcgggaagt gcggttcgct cgctgatctg
     2041 ggctcgcgca ccgcagcttt tcggagcggt ggctct
//
LOCUS       DASRJQ010000284         2647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1112728, whole genome shotgun sequence.
ACCESSION   DASRJQ010000284 DASRJQ010000000
VERSION     DASRJQ010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112728"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VFB89_06375"
     CDS             <1..556
                     /locus_tag="VFB89_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26962.1"
                     /translation="FPRNPGVTSQDLEPSVQAIVLERRRAVRGDSAVLADILSRIPKR
                     DYDHGDAWVLGRLAAGARDSVSNRFLAADSLEFRVRYLTLLSYFTDPALIPLFSRVYI
                     WPDSFNIPLRYAVRASDGLVWIGTRESLAALLDARARAKARGIYADSSLSRGGYSFLE
                     NDSAAVLARTGKWLTDWLKTLPAH"
     gene            complement(590..1972)
                     /locus_tag="VFB89_06380"
     CDS             complement(590..1972)
                     /locus_tag="VFB89_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26963.1"
                     /translation="MGFGLRRKESNARFGHAVVVGGSMAGLLAARVLADHFERVTLIE
                     RDQLPDTPEYRVGDPQARHAHALLTRGQLILEQLFPWICRELVDAGGVALDTARDLAW
                     LTPAGWGARFASDLIAVSTTRALLEWTVRRRLREHPEVRIRDGVAIRGLIRSADGSAI
                     SGVRLASQAPDTIPADLVVDATGRGSRLPEWLAWLRFRRPAETTIDASLGYASRILRL
                     RGGFRLDASGVFVQAAPPEHPRGGALFPIEGNRWLVSLFGGGGHCPPTDEAGFLEFAR
                     ALRTPLIAQAIASADPLSDIVGTRATANRRRHYEKLSGMPDGVIALGDSVCAFNPVYG
                     QGMSVAAIGAEVLDLSLRAQRAAGRAELGGFGRKFQRRLARVIDTPWSLATGEDYRYR
                     DVKGPRPSWSVRLMHSYVDRLVAASTVNPSVRARLMQVLGMMRGASLLFGPAMLGSVL
                     LRNAAASAGR"
     gene            complement(2101..>2647)
                     /locus_tag="VFB89_06385"
     CDS             complement(2101..>2647)
                     /locus_tag="VFB89_06385"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HZM26964.1"
                     /translation="TVPAAIASFTFSLLGAHAVSILDPTILRPLILALLVVVVVVTWR
                     ARDFGTVHLPRLATATALAAAMLMGAVLGFYDGFFGPGTGTFLIFAFVGLFGFDFLGA
                     TSAAKVVNLGSNFSAALYFVLTDQVIYRLAVPMAVASIAGGTVGSRLALARGVALVRV
                     FFLVLVTALILKLAFDTLHQS"
BASE COUNT          510 a          861 c          873 g          403 t
ORIGIN      
        1 cttcccgcgg aatccgggag ttacctcgca ggacctcgag ccatcggtgc aggcgatcgt
       61 gttggagcgc cgccgagccg tacgcgggga ctcagcagtg ctggccgaca tcctatcgcg
      121 cattcccaag cgggactacg accacggtga cgcctgggtg ctgggacgac tcgccgccgg
      181 tgcccgcgat tcggtgagca atcgcttcct tgccgctgac tcactcgagt tccgggtccg
      241 ctacctcacg ttgctgtcct acttcaccga cccggcgctg atccccttat tttccagggt
      301 gtacatctgg ccggacagct tcaacatccc gcttcgctat gccgtccggg cgtcggatgg
      361 attggtgtgg atcgggactc gcgaaagcct ggcggcgctg ctcgatgctc gagcgcgggc
      421 caaggcgcgc ggcatctacg ccgattcgag cctttcccgc ggcgggtaca gctttctgga
      481 gaatgacagc gcggcggttc tggcgcgaac cggaaagtgg cttaccgact ggctgaagac
      541 gctaccggcc cactgatccg aagctcgcgg ttagacctgg gccgcctggt catcggccgg
      601 ctgatgcggc ggcattccgg agcagcacag atcccagcat ggcagggccg aataggaggc
      661 tggcccctcg catcattccc agcacttgca tcaacctggc tcgtaccgat ggattcaccg
      721 tcgaggcggc taccagccga tcgacgtagc tgtgcatcaa gcgaaccgac cacgaggggc
      781 gcgggccttt cacgtcgcga tagcgataat cctcaccggt cgcgaggctc cagggcgtat
      841 cgatgactcg ggccaaccgc cgctgaaact tgcggccgaa gccgcctaat tctgctcttc
      901 cagccgcccg ctgcgctcgg agactcaggt ccagtacctc cgcgccgatt gccgcaaccg
      961 acataccctg accgtacacc ggattgaaag cgcacaccga gtcacccaac gcaatgactc
     1021 cgtcgggcat ccccgacaat ttctcgtagt gacgccggcg attcgccgtc gcccgcgtcc
     1081 ccacgatgtc ggagagcggg tccgcgctcg cgatagcttg cgcaatgagc ggcgtgcgaa
     1141 gcgccctcgc aaactcgaga aagccggctt catcggtggg cgggcaatgg ccaccgcctc
     1201 cgaacaggct caccagccag cggttccctt cgattgggaa gagcgcgccg ccacgggggt
     1261 gctccggcgg cgccgcctgg acgaacaccc ctgatgcgtc gaggcggaag ccgccccgca
     1321 gtcgaagaat gcggctggcg tagccgagcg atgcgtcgat ggtcgtttcg gcgggacggc
     1381 ggaatcgcag ccaggccaac cactcgggca agcgcgagcc tcggccagta gcgtccacga
     1441 ccagatctgc cggtatggta tcgggcgctt gactcgcaag acgcacgccg ctgatggccg
     1501 acccatcggc tgagcggatc aggcctcgaa tggcgacccc gtcgcggatc cgcacttccg
     1561 gatgctcacg aagacggcgg cgcaccgtcc actcgagcag cgctctcgtc gtggagacag
     1621 cgatgaggtc ggacgcaaag cgcgcacccc atccggctgg tgtgagccag gccagatcgc
     1681 gagccgtgtc cagcgcgaca ccgccggcgt ccaccagctc gcggcagatc caggggaaaa
     1741 gctgctcgag gatgagctgg ccgcgggtga gcagagcgtg ggcgtgccgc gcctgcggat
     1801 cgcctacccg gtactccggc gtgtccggca gctggtcacg ttcgatgagc gtgacgcgct
     1861 cgaagtgatc ggccagcacg cgtgctgcga gcaatcccgc catgctgccg ccaaccacga
     1921 cggcgtggcc aaaacgagca ttggattcct ttcgacgtag cccaaatccc atatccgtgc
     1981 ccaggtgaag ggacgacctc agattgctgc gtcggcgtcg cgcggggcat cggttgggcc
     2041 acagcggaga ggtagggatt ggcgtcgacc ttttgtcagt cgcaggccga caccatggcg
     2101 tcagctctga tgcagggtgt cgaaggcgag cttgaggatg agggcagtga ccagcacgag
     2161 gaagaagacg cggaccaacg ccacgccgcg ggcgagagca agccgactgc ccacggtccc
     2221 tcccgcgatg ctagcgaccg ccatgggaac ggcgagccga tagatcacct gatccgtcag
     2281 cacgaagtaa agtgcggccg agaagtttga gcccagattg acgaccttgg cggcggacgt
     2341 ggcgccgagg aagtcgaagc caaaaaggcc cacgaaagca aagatgagga aggtgccggt
     2401 tccgggtccg aagaagccgt cgtagaagcc gagcacggcg cccatcaaca tcgccgcggc
     2461 cagcgcggtc gccgtcgcca gacgggggag atggaccgta ccgaagtcac gtgcccgcca
     2521 agtaaccacc accaccacaa caagaagagc gaggatcaac gggcggagga tcgtcggatc
     2581 cagtatgctc accgcgtggg cgccaaggag agagaaggtg aacgaggcga tggcggcagg
     2641 aacggtg
//
LOCUS       DASRJQ010000285         2162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2015060, whole genome shotgun sequence.
ACCESSION   DASRJQ010000285 DASRJQ010000000
VERSION     DASRJQ010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2015060"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(859..1080)
                     /locus_tag="VFB89_06390"
     CDS             complement(859..1080)
                     /locus_tag="VFB89_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26965.1"
                     /translation="MANLARAVVPVPTPNRDRMGVDPPIEVESATWVAGGTLDWWIKE
                     RLEWFGRVRGKDGRQRWINTADLRPAKSE"
     gene            complement(1080..1475)
                     /locus_tag="VFB89_06395"
     CDS             complement(1080..1475)
                     /locus_tag="VFB89_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26966.1"
                     /translation="MRGDGRLMRVAWVCLVIVSVGILGFGVITVLPSVAGGNLLIVAN
                     GLASIGLGLFGGLIVLIPFRRGERWAWLVLWFYPLFWLVHLVAELPPGKDHIHQIVFI
                     VLSAAGLLLPVRDMIGAGRPPVPRTRSGA"
     gene            complement(1536..2117)
                     /locus_tag="VFB89_06400"
     CDS             complement(1536..2117)
                     /locus_tag="VFB89_06400"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HZM26967.1"
                     /translation="MLFAAFLPALAIMILLNNVAEEIGWTGFVFARFQDRHGPLRAAL
                     LTTVFFWLFHVPSTYVETRSWATTALVLGIFVLPHLGSRLITGWLYNSAGASVLIAGL
                     FHAMHNAIVNATGLVAVVSLPQFEVLVIMAGIVVIAAAIITVATRGRLGLKRSSGRSA
                     TLVRPGTEKRARRGIRHREDPVSAAPADDDYPT"
BASE COUNT          454 a          674 c          665 g          369 t
ORIGIN      
        1 atcccacgtc aggggaggtt gcctggcccc aagaaatgtg tcgcgcagct cttttcggaa
       61 gacgtcgacc tccgccgtcg cgtagccctc tcgcaggcgg ggggtggttg aaaatctcgt
      121 tgagtgcagg atcgaggact cgcgcgctgg ttccgatcga gggtatcgat atccgttcag
      181 acccgctgcg gccgactggt cgagctcgag cggccattgc aagggctcgc ccccatcagt
      241 ggcgcgagct cagaccctcg cggacgatca cgccgcagat gatcttgccg agccaagatc
      301 tgggcctgat ttgttgagac ggctgcgcca aatcgcgcgc tcgatcttga agagccacgg
      361 ctcaatgccg tccgtgggca accgggtgct ccgaagatga tcttgactcg ccgctggacg
      421 ggatcggcca cgaccgcttc gatcgaacag gcatgaagtc gcctccgcgc aaaaggattt
      481 gcgcacacaa aagggtaagt tcaaggggag actccagcta gctacaggcc ttcagcgcca
      541 actagctgga gcctctcccc ctgtacgagc ccaagttccc acgctcgcgc cgagggtcgt
      601 ggcgcgtcaa gagggctgag tcatcgagtt catagcgtgg tcctctttcc tcgaaaccga
      661 atctgtgttc gaatcagatg tgagggatct cgatctgagt cagtacgccg aacacacctt
      721 cgagcgaggc gccaatggga agacatggat gcccgccgac gcgtaggcga gagcggcggc
      781 caggtgagcg acgggctcag cctgtttgtg cggtgaccta ccggtcggcg aatgacgcat
      841 ggcaacgaca agtcatggtt actcgctctt tgccggacga agatcagcag tgttgatcca
      901 ccgctgacgt ccgtctttgc cgcgaacgcg gccgaaccat tccaggcgct ccttgatcca
      961 ccagtcgagc gtgcctccag cgacccacgt agctgactcg acctcgatcg gtggatccac
     1021 tcccatgcgg tcacgattgg gtgtggggac aggcaccacg gcgcgggcga ggttggccat
     1081 caggcgccag accgggtgcg cggcacaggc ggtcgacccg ctccgatcat gtctcgaaca
     1141 ggtagtagca gaccggccgc ggagagcacg atgaacacaa tctggtggat gtggtccttg
     1201 cccgggggca gttcggcgac caggtgcacc agccagaaca gaggatagaa ccacagcacc
     1261 aaccacgccc acctctcgcc gcgacgaaac gggatcagca cgatcagccc accgaacagg
     1321 cccagaccga tcgatgccag cccattcgcg acgatcagca agttgccgcc ggcaacagac
     1381 ggcagcacgg tgattactcc gaagccaagg atcccgacgc tcacgatgac caggcacacc
     1441 cacgcaactc gcatcagccg accgtcgcct ctcacttggc gaagctaact gatccagcgc
     1501 tgcttaccgt ccttgccccg cacccgataa caggcttatg tcgggtaatc gtcgtctgcg
     1561 ggtgccgccg agacgggatc ctctctatgt cggatgccgc ggcgtgctct tttctcggtg
     1621 ccgggccgga cgagggtcgc gctgcgtcct gatgatcgct tgagaccgag acggcctcgg
     1681 gtggcgacgg tgatgatcgc ggcagctatc acgacaatcc cggccatgat caccaacact
     1741 tcgaactgtg gcaaactaac cacggccacc aggccggtgg cgttcacgat ggcgttgtgc
     1801 atggcgtgga acaatccggc gatcaacacg ctggcaccgg cgctgttgta aagccagcca
     1861 gtgatcaatc ggctgcctag atgcggtagt acgaagatgc ccagcaccaa cgcggtggtc
     1921 gcccaggatc gtgtttcgac ataggtgctc ggcacgtgga acagccagaa gaaaaccgtc
     1981 gtcaacaagg ctgcgcggag cggaccgtga cgatcttgga accgggcgaa aacgaatccg
     2041 gtccatccga tctcctcggc gacattgttc agcaagatca tgatcgctag tgccggcagg
     2101 aacgcggcga aaagcagcga ccaattctgg caagagcgcg cagcggcgcc agaccatcaa
     2161 gg
//
LOCUS       DASRJQ010000286         2253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1733856, whole genome shotgun sequence.
ACCESSION   DASRJQ010000286 DASRJQ010000000
VERSION     DASRJQ010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1733856"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..>2253
                     /locus_tag="VFB89_06405"
     CDS             62..>2253
                     /locus_tag="VFB89_06405"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZM26968.1"
                     /translation="MRLAAHFGMVPYVAESETRAISPGSHSGRAILERRVIHVHDMLD
                     VRSRADYPDVVKLGRLSGTRTVLAMPLLREGTPIGVIVIRRREVRPFSDEQIDLVKTF
                     ADQAVIAIENVRLFTELQQKNEALAQAHAQVTGALERQIATSEILGVISSSPTDVRPV
                     FQAIARNAVALCGGLGAVVLRYDGTLLHVAAHHNVSAEAIAMVERAYPRRPARDYPPG
                     RALLDGTVVHVPNLREATEFTASTARERGAASFLAVPLLREGEAIGVIGLARDIIGSF
                     PSQQVTLLQTFADQAVIAIENVRLFTELQEKNRALTQAHAQTTEALDQQTATSEILRV
                     ISSSPTDLQPVMDTIVANAARLTDAQSALIGRADGNVIRWLAFYGAPPSGPQPITRSL
                     PSGRAILDRQTTQVEDIATIEKGQFTEPVLRAYREHGVRTVLATPLLRNDAALGVLLV
                     RRTVVRSFTDKQIALLQTFADQAVIAIENVRLFAELQASNRELASKSRQLEVASQHKS
                     EFLANMSHELRTPLNAIIGFSEVLSEKMFGDLNDKQDEYLKDIHASGQHLLSLINDIL
                     DLSKIEAGRMELEPTDCHLPATLDNALSLVRERAGRHGIALHAAIDDGIEYIRADERK
                     IKQVLLNLLSNAIKFTPEGGQIEIRAKSVNGSIEVSVSDTGIGIAPEDQEAIFEEFQQ
                     VGTAAKKVEGTGLGLALSRKFVELHGGRISVQSELGAGSTFTFTLPLR"
BASE COUNT          457 a          698 c          700 g          398 t
ORIGIN      
        1 gagcgccccc cgcctgtgcg cagcgaatga cgtcctaatc catcgcgtcg aaggcggcat
       61 catgcggttg gcagcacatt tcgggatggt gccgtacgtc gcagagtcgg agacgcgagc
      121 gatctcaccg ggctcgcatt ccggccgggc aatcctcgaa cgccgagtga tccacgtgca
      181 cgacatgctc gacgttcggt cccgcgccga ctatccggac gtcgtcaagc tgggacgtct
      241 gagcggaaca cggacggtgc tggctatgcc cttgctccgc gagggtacgc cgatcggggt
      301 catcgtgatc cgcaggcgcg aagtgcggcc gttctcggac gaacagattg acctggtgaa
      361 gaccttcgcc gatcaggccg tcatcgccat cgagaatgtg cggctgttca ccgagctgca
      421 acaaaagaac gaggcgctgg cgcaagctca tgcccaggtc acaggggcgc tggagcggca
      481 gattgcgacg tccgaaatcc tcggagtaat cagtagctcg ccgacggatg ttcggcccgt
      541 cttccaagcg atcgcgcgga acgcggtcgc gctctgcggc ggcctgggtg ctgttgtcct
      601 gcggtatgac ggcacgttgc tgcatgtggc tgctcatcac aatgtgagcg cggaagcgat
      661 agccatggtt gaacgggcct atccccgccg ccccgcacga gactatccgc ccggccgggc
      721 cttgctcgat ggcacggtgg tccacgtccc gaatcttcgg gaagccaccg agttcactgc
      781 ttccacggcg cgagaacgcg gtgctgctag cttcctcgct gtaccacttt tgcgcgaggg
      841 agaggcgatt ggggtcatcg gcctcgcccg ggacattatc ggaagtttcc cgagtcagca
      901 ggtcactctg ttgcaaacct tcgccgacca agcggtcatc gcgatcgaga acgtgcgact
      961 gttcaccgag ctgcaagaga agaaccgtgc tctcacgcag gctcatgcgc agacgaccga
     1021 ggctttggat caacagacag cgaccagcga aatcctgcgt gtgatctcga gttcgccgac
     1081 cgatcttcag ccagtgatgg acacgatcgt ggcgaacgcc gcccggttaa cggacgccca
     1141 gagtgcgctc atcggacgcg cggacggaaa cgtcatccgc tggctcgcct tctatggcgc
     1201 gccaccgtcg ggcccgcagc cgatcactcg aagtctcccc tcggggcgtg caatattgga
     1261 tcggcagacg actcaagtcg aagatatcgc caccattgaa aagggccaat tcaccgaacc
     1321 agtgctccgg gcgtatcgcg agcacggggt acgaacggtt ctcgccaccc ccctcttgcg
     1381 gaacgatgca gccctcggcg tcctactcgt aagacggaca gtagtccgtt cgttcaccga
     1441 caaacagatc gcgctgctcc agaccttcgc cgaccaggcg gtgatcgcca ttgagaatgt
     1501 tcggctgttc gctgagcttc aggccagcaa ccgcgaactc gctagcaaga gccggcagct
     1561 ggaagtcgcc agccagcaca agtcggaatt cctcgcgaac atgtcgcacg agctacggac
     1621 tccgctgaac gccatcatcg ggttctcaga ggtcctgagc gaaaaaatgt tcggcgacct
     1681 aaacgacaag caggacgagt acctcaagga tatccacgcg tccggccagc acctcctctc
     1741 gctgattaat gacatcctcg acctgtcaaa gatcgaggcg gggcggatgg agctggagcc
     1801 gacggactgc catctgccgg cgacgctcga caatgccctc agccttgtgc gcgagcgggc
     1861 cggacgtcac ggtattgcgc tgcacgccgc cattgacgat ggcattgagt acattcgggc
     1921 ggacgagcgc aagatcaagc aggtgctcct caacctgctc tcgaacgcga tcaagttcac
     1981 gccggaaggc ggccagatcg agatccgagc gaaatcggtg aatggatcca ttgaagtgtc
     2041 cgtgagtgac accgggatcg gcatcgcgcc agaggaccag gaagccatct tcgaagagtt
     2101 ccagcaggtc ggaacggccg cgaagaaggt ggaaggcaca gggctcggct tggccctgtc
     2161 gcggaaattt gtagagctgc acggtgggcg gatctcggtt cagagcgagt tgggcgcggg
     2221 ctcaacgttc acgtttacgc tcccactgcg cca
//
LOCUS       DASRJQ010000287         3701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2203113, whole genome shotgun sequence.
ACCESSION   DASRJQ010000287 DASRJQ010000000
VERSION     DASRJQ010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2203113"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /locus_tag="VFB89_06410"
     CDS             complement(<1..1179)
                     /locus_tag="VFB89_06410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZM26969.1"
                     /translation="MRLALSLAAVWLIGLLTLLCLGWGRTATTGRGRSVLTCLAIGIG
                     LGVSSSLFVVWLLFGAPAAAFPYAESVLLVLLAVGAFVGRSRNRRPSQYLDRSSQVGR
                     PVLSLVLSLSVACAVIAFVAEWMTNRQGAWDAWMTWNMHARALFRGGEQWREVLTGLP
                     AWSHPDYPLLVPGSVARVWTYAGQETTFAPGAVAMLFTFATIGLLYSAVASLRSRTQG
                     VLAALVLLGTKFYILQGASQYADIPLSFFFLATLALLSLGELSESNRSRLVMMAGVAA
                     GLSAWTKNEGTLFVLALVIAYGLVMGPALGWRTALATLRSFAIGLGPAVLLLAFFKIN
                     LAPANDLLSDHGLRQSAERLFDGGRYVQILTGFTQAVLEVGAKGLLVVLLAAYLLAVG
                     S"
     gene            complement(1176..2573)
                     /locus_tag="VFB89_06415"
     CDS             complement(1176..2573)
                     /locus_tag="VFB89_06415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZM26970.1"
                     /translation="MSGTVRISVVIPTYQRRTSIQRALDALGRQTLPATEFEVIVPID
                     GSDDGTKEMLDGFQAPFRLTAIWQPNQGRAAARNAGTRLANGELIVFLDDDMEPVPGF
                     LQAHLDAHPAGSRRAVVGPVPIPADSSSAPIVSYRRDATNALLARLGQPGYRLGFRDV
                     YTGNLSLQREALRQVGGLDETFKLYGHEDYELAFRLAKAGVEFGYSPDAIAYQQYDKT
                     FAGLARDCVDRGHTAVLFARKHPEIASGLRLNSYDEGPYRWRLARSLFLWLSGLFPRF
                     PERLVAAMAWLERRQHRRLHHYYTLALDYFFWLGATSALRESGAQATRRTALAALALA
                     CLVLFALGSSVRLLGRAVHALRQLPSRDAITSYEARFRDLRPDLPRVARVGYVTDRDI
                     GPGEGLDAPRLAFKRYLLTQYALLPTIVLSDTQAGLVVGNFAAPGSPDSNATAGLSLI
                     RDYGNGVMLFRSQAQ"
     gene            complement(2570..>3701)
                     /locus_tag="VFB89_06420"
     CDS             complement(2570..>3701)
                     /locus_tag="VFB89_06420"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZM26971.1"
                     /translation="REALDRVGGFEESFVGPVQTFEDDAFLAKVQLRESVFVATECWS
                     RYRRHENSLLSIMTNTGRTRGARLFYLRWLEQYLAQQGVDSDEIRDALSKAFLPYNHP
                     VRHALRNAGSQFSVKRIRGIVKKAARRMLPARTHRWLRAQWERRRHRPRLGSVQFGEL
                     RRVTPVSRNLGRDRGTPIDRYYIEQFLARNAECINGHVLELDDDRYTRRFGNSRVARS
                     DVLHVNPTNPRATIVADLTDAGHIPSDRFDSIILTQTLPFIYDIHAVMRTLYRILKPG
                     GTILATVGGITQISRRDMQRWGHYWSFTTLSVRLLFEEVFPAPSVRVEAFGNVLATTA
                     FLHGLAAEELRADELDYRDPDYEFLITITAVKPRNEVAEPAE"
BASE COUNT          746 a         1181 c         1203 g          571 t
ORIGIN      
        1 gctaccaacg gcgagcaggt aggctgcgag caacactacg agcaggccct tggccccaac
       61 ctccagcaca gcctgggtga aacccgtgag aatctggacg taccgcccgc cgtcgaagag
      121 acgctccgcg ctttgtcgga gtccatgatc ggacaagagg tcgttggcgg gcgcgaggtt
      181 gatcttgaag aaggccagca gcaggaccgc cggtcccagg ccgatcgcga agctccgcag
      241 cgtggcgagt gccgtacgcc agccaagggc tggccccatc accaggccat acgctatcac
      301 cagagcgagg acgaaaaggg tgccctcgtt cttggtccag gccgagagtc ctgccgcaac
      361 cccagccatc atcaccaatc gagatcggtt cgattcggaa agctcgccga gcgacagcag
      421 cgcaagcgtc gccagaaaga aaaaactgag cgggatatcg gcgtactgcg acgcaccctg
      481 gaggatgtag aacttcgtgc cgagcagaac cagtgcggcg agaacaccct gagtgcggga
      541 ccgaagggag gcaaccgcag aatacaggag cccgatggtg gcgaaggtaa agagcatggc
      601 caccgcaccg ggcgcgaacg tcgtctcctg tcccgcatag gtccagacgc gggcaacgct
      661 cccggggacg agcaggggat aatccggatg cgaccacgcg gggaggccgg tcaggacctc
      721 acgccattgc tcgccgccgc ggaagagggc tcgggcgtgc atgttccacg tcatccaggc
      781 gtcccacgcc ccttgccgat tggtcatcca ctcggcgaca aaggcaatga cggcgcatgc
      841 gacgctcagt gacaggacca ggctgagcac cgggcgaccg acttgggaag agcgatcaag
      901 atattgcgag ggccggcggt tccggctgcg cccaacgaaa gcaccaaccg cgagcaacac
      961 cagcaatacc gactcggcgt aggggaatgc tgcggccggc gcgccgaaca gcagccatac
     1021 cacgaagagt gacgagctga ccccgaggcc gatgccgatc gcaaggcagg tcagcaccga
     1081 ccgaccccga cccgtcgtcg cggtcctccc ccaccccagg cacagcagtg tgagcaggcc
     1141 gatcagccag accgctgcca gcgacagcgc gagcctcact gtgcctggga tcggaacagc
     1201 atgacgccgt tcccgtagtc gcggatcaag ctgagtccgg cggtcgcgtt gctgtcgggg
     1261 ctccccggag ccgcgaagtt acccactaca agaccagctt gtgtgtcgga aagaacaatc
     1321 gtcggcagga gcgcatactg cgtcagcaga tatcgcttga acgcgagccg cggggcgtcg
     1381 agcccctctc ccggcccgat gtcgcggtcg gtgacatatc ccacgcgcgc tacccgaggg
     1441 aggtccggtc gaagatcacg aaagcgggcc tcgtagctcg tgatggcatc gcggctgggg
     1501 agctggcgta gcgcgtgaac ggcgcggcca agaaggcgca cgctggaccc cagggcgaac
     1561 aacacgaggc aggcgagcgc cagcgcggca agtgcagtcc gacgagtcgc ctgtgcaccg
     1621 ctttctcgca gagccgacgt tgctcccagc cagaagaagt agtcgagagc cagagtgtag
     1681 tagtgatgaa ggcgtcggtg ctgccggcgc tcgagccacg ccatcgctgc caccaggcgc
     1741 tccggaaacc ggggaaaaag cccgctcagc cacaggaaca gggaacgggc caatcgccac
     1801 cggtacggtc cctcatcgta cgagttcagc ctgagaccgg aggcgatctc cggatgcttg
     1861 cgagcgaaca acacggcagt gtgcccacga tcgacgcaat cccgggcgag gcccgcaaag
     1921 gtcttgtcgt attgctggta cgcgatcgcg tccggactgt acccaaactc gacgccggct
     1981 ttggccagcc gaaacgcaag ctcgtaatct tcgtggccgt agagcttgaa ggtctcatcc
     2041 aaaccaccga cctggcgcag ggcctcgcgc tgcaatgaca ggttgccggt gtagacgtcc
     2101 cgaaagccaa gacgatatcc cggctgaccc agtcgtgcga gcaaagcatt cgttgcgtcg
     2161 cgccgatagc tcacaatcgg cgcggaggat gagtcggccg ggatgggaac cggcccgacg
     2221 acggctcggc gagacccggc gggatgagcg tcgaggtgag cctggaggaa gccgggtacc
     2281 ggctccatgt cgtcgtccag gaacacgatc agctccccat tcgccagacg ggtgcccgcg
     2341 ttgcgcgccg cagcccttcc ctggttgggc tgccagatgg cggtgagccg aaacggggcc
     2401 tggaacccgt ccagcatctc cttggtcccg tcgtccgacc cgtcgatcgg gacaatcacc
     2461 tcgaactccg tcgccggcag cgtctggcgg ccgagtgcat ccagcgcccg ctggatggat
     2521 gtgcgtcgct ggtaggttgg gatgacgacg ctgatgcgga ccgtgccgct cattcggcgg
     2581 gctccgcaac ctcgttgcgc ggcttcacag ccgtgatcgt aatgagaaac tcgtagtccg
     2641 ggtcccgata atcgagctcg tcggcgcgaa gttcctccgc ggcaaggccg tgcagaaacg
     2701 cagtggtcgc gagcacgtta ccgaaggctt cgactcggac gctcggagcc gggaacacct
     2761 cctcaaacag caggcgcacc gacaatgtcg tgaaactcca gtagtgaccc cagcgttgca
     2821 tgtcccgccg gctgatctgc gtgatgccgc cgacggtggc gagaatggtc ccacccggct
     2881 tcaggatccg atagagggtc cgcattaccg cgtgaatgtc atagatgaaa ggcaacgtct
     2941 gcgtcaggat gatgctgtcg aaccgatccg acggaatgtg gccggcatcc gtgaggtccg
     3001 cgacgatggt cgcacgcgga ttggtcggat tcacgtgtag gacatcgctc cgggcgaccc
     3061 ggctattccc gaacctgcgg gtgtagcggt cgtcgtccag ctccagcaca tgcccgttga
     3121 tgcactcggc gttccgcgcc aggaactgct cgatgtagta gcgatcgatc ggcgttcccc
     3181 ggtcgcggcc gagattacgg ctgaccggcg tcactcggcg aagctcgccg aactgtaccg
     3241 acccgagccg aggtcgatgc cggcggcgtt cccattgggc tcggagccag cgatgagtgc
     3301 gggcgggtag catgcgcctc gccgccttct tgacaatgcc ccttatacgc ttgaccgaga
     3361 attggcttcc cgcattccgg agcgcgtggc ggaccgggtg gttgtagggc aggaaagcct
     3421 tgctcagcgc atctcgaatt tcgtcggagt cgactccttg ttgtgcgaga tactgttcga
     3481 gccagcgcag atagaaaagc ctcgctcccc gggtccggcc cgtgttggtc atgatcgaga
     3541 gcagcgagtt ctcgtgccgc cggtaacggc tccagcactc ggtcgcgaca aaaaccgact
     3601 cgcggagctg gacctttgcc aggaaggcat catcctcgaa ggtctgaacc gggcccacga
     3661 aggattcctc aaaaccgccc actcggtcca aggcctcgcg c
//
LOCUS       DASRJQ010000288         3908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_890463, whole genome shotgun sequence.
ACCESSION   DASRJQ010000288 DASRJQ010000000
VERSION     DASRJQ010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890463"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1791
                     /locus_tag="VFB89_06425"
     CDS             <1..1791
                     /locus_tag="VFB89_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26972.1"
                     /translation="HLVLRELAQRLYRRQLGGITFSLGVEPAEFVELLRALGAEGSSV
                     SAESAEATQWPHVRLHPMAFDQLRLVGDADRGGSRVSADSNQLWNDLASSALGLTSDS
                     DDEVADAGTVAQAIDARVRDPEYASALTRQLLGMVRHAQSSEAAEAKAVGRQLVDLVR
                     SLKPETLRQLLALGPDRGDREQLARAMSRTMPLTAVIDLVRASSEASEQTISHSLLRI
                     LAKLASHAEAESGGTKPDDALQDMVRELLTDWALKDPNPDAYRAMLEGFARPGGRAAA
                     SAGARAGDLMEPLRVVQLSIELNVASESLTTAVDTLVERKETPALLNLLAQAPAGEAA
                     AEAVWSRLPTRDELRRALEREEPDTALVERLLRRLGIEAAEPLLDALGGADSRNTRRR
                     LLAWLAQLGPGVGPLIMARLESPHWYVLRNMLVLLGGLDHWPEEFSPSAYLAHADARV
                     RREALKLALRVPELRDQAIRAGLADGDDLILRTALAAALDGCPVEAAPRLVELTESRG
                     QAADVRLQIVKVLASLRTPAARDCLIKRVLTRRGWVPWRRLAPKSSELVVAVSGLATQ
                     WADDSKAVHVLRVAARSSDPEIRSAAGVAS"
     gene            1788..2969
                     /locus_tag="VFB89_06430"
     CDS             1788..2969
                     /locus_tag="VFB89_06430"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZM26973.1"
                     /translation="MSDPYRYLTSLGQALAAMSLYPAGHRARERAVESSFEQLEELAR
                     QEAQPQFSFLDGEVIYRRQIIRELTGWEWGKRLAAAGMQRLEFLEGVSREEYADWLDE
                     IQRLLAGEAPDTSEARQLIARRIRYGAVEVRGGGSTEKSVAVGAARDPLAFPDLDAEV
                     EAIRWIHGEVESLGRLPLLEAEAVVRSLTLAMHSQSRIRLPLLQLKTFDQYTTTHSTN
                     VAVLAMAVAEYLGLGAREIRSFGVAGLLHDLGKVRIPRDILVKPGKLTDEERAVMQSH
                     PVDGARIILEQDMQLDLAAFVAYEHHIMLNGGGYPTLRYPRDAHYASKVVHVCDVYDA
                     LCTHRPYREAWASERALGYLAERVSTEFDPDLVDAFTRMIGDWEQRRSPIDEKSVNGE
                     Q"
     gene            complement(3023..3649)
                     /locus_tag="VFB89_06435"
     CDS             complement(3023..3649)
                     /locus_tag="VFB89_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZM26974.1"
                     /translation="MRRVLVVASSPVIRAGLETLLARAGDYTVLGSARLPGLPTSENA
                     AAPDVIVLALDADGDAGPVAQLAADQGTSAPPLVVIGDEPVVEWGARALRLGARAVLS
                     HAAGPAEIVAAVEAAAVGLVALPPELIGNAARPPRAADAVPQTLTAREIEILGLLAAG
                     LANKTIGARLGISEHTVKSHVAALFAKLGVSTRAEAVAVAVRQGVLML"
     gene            3697..>3908
                     /locus_tag="VFB89_06440"
     CDS             3697..>3908
                     /locus_tag="VFB89_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26975.1"
                     /translation="MGDPFEAAYDLNLQEVAWASLLQRISQAIRMIEPITADGREHIA
                     DDDAGAVSTAPACDLEDEEANSGGLAA"
BASE COUNT          653 a         1259 c         1332 g          664 t
ORIGIN      
        1 cacctcgtgc tgcgcgagct ggcccagcgg ctgtaccggc gccaactggg cggaatcacc
       61 ttctcgttag gggtcgaacc cgccgaattc gtcgaactgc tgcgcgccct cggtgccgag
      121 gggtcgtccg tgagtgccga atctgccgaa gccacgcaat ggccgcatgt gcgccttcac
      181 cctatggcat tcgatcagct gcggctggtc ggagatgcgg accgaggcgg atcgcgcgtt
      241 tctgccgatt ccaaccaact atggaacgac ctggcgagct cggcgctggg actcaccagc
      301 gacagtgacg acgaggttgc cgacgcaggt acggtcgcgc aggccatcga tgctcgggtg
      361 cgcgatcccg aatatgccag cgcattgacc cggcagctgc ttggcatggt ccgtcatgcg
      421 cagtcgagtg aagccgccga ggcgaaagca gtaggcaggc agttggtgga cctcgtgagg
      481 agcctcaagc ccgagaccct gcgacagttg ctggctttgg gcccggaccg gggtgatcgc
      541 gagcagcttg cgcgggcgat gtcccggacc atgccgctaa cggcggtcat cgatctcgtc
      601 cgggcctcgt cggaagcgag cgagcagacc atctcgcact cgctgctgcg gattctggcc
      661 aagctcgcgt cgcacgcaga ggccgaatcc ggggggacga agcccgacga cgctttacag
      721 gacatggtcc gcgagctcct aacggactgg gcgctcaaag atcccaatcc ggatgcctac
      781 cgagcgatgc tggaaggctt cgcgcgtccg ggtggtcggg cagcagcttc cgcgggggcg
      841 agggcgggcg acttgatgga gcctctgcgt gtggtgcagc tgtcaatcga actcaacgtg
      901 gcgagtgaat cgctgaccac agcagtcgat acccttgtcg agcggaaaga gactccggca
      961 ctgctcaacc tgctcgctca ggcgccggcc ggcgaggcag ccgcagaggc cgtctggtcc
     1021 cgacttccta cccgcgacga gctgcgtcgt gcgctcgaac gggaggagcc cgatacggcg
     1081 ctggtcgaac gcctgctgcg ccgactgggg atcgaggcgg cggagcccct gctggatgcg
     1141 ttgggtggag cggactcccg taatacccgt cggcgccttc tcgcgtggct ggcgcaactg
     1201 ggtccggggg tcgggccgct gatcatggct cgtcttgaat cgccgcactg gtacgttctc
     1261 cggaacatgc tggttttgct gggcgggctc gatcactggc cggaggaatt ctcgccgtct
     1321 gcgtacttgg cgcacgctga cgcgcgggtc cggcgtgagg cgctcaagct ggccctgcga
     1381 gttccggagc tgcgagatca ggcaatccgg gccggcctgg ccgacggcga cgacctgatt
     1441 ctgcgtacgg cgctcgctgc ggcgctcgat ggctgcccgg tggaagctgc tccccgattg
     1501 gtcgagctga cggagtcgcg aggccaggct gccgacgtgc gcttgcaaat cgtcaaggtg
     1561 ctggcgagcc tgcgaacgcc ggcggcacgc gattgcctga tcaaacgcgt tcttacccgc
     1621 cgcgggtggg taccgtggcg ccgccttgcc cccaagtctt ccgagctggt tgtggcagtg
     1681 agcggcctcg ccactcaatg ggcggacgat tccaaggccg tccatgtgct gcgggtcgcc
     1741 gcgcgcagtt ccgaccccga gatccgtagc gctgcggggg tcgcttcatg agcgacccct
     1801 atcgctatct cacctcgctc gggcaggcat tggctgctat gagtctctat cctgccgggc
     1861 accgagcgcg ggagcgagcg gtcgaatcgt ccttcgagca gctggaggag ctcgctcggc
     1921 aggaggccca accgcagttc tcatttcttg acggcgaagt gatttaccgc cggcagatca
     1981 ttcgcgagct gacgggatgg gagtggggca agcggctcgc ggcagccggg atgcagcgcc
     2041 tggaatttct cgagggtgtc agccgcgaag agtatgcgga ttggctggac gagatccagc
     2101 ggctactggc aggggaagca cccgacactt ccgaggcgcg tcagctgatt gctcgccgca
     2161 tccgatacgg cgcagtcgag gtgcgaggcg gcggtagcac cgagaagtcc gtcgctgtcg
     2221 gcgccgcccg cgatccgctg gcctttccgg acctggacgc ggaagtggag gccatccgct
     2281 ggattcacgg cgaggtggaa tccctgggtc ggcttccgtt gctggaggct gaggctgtcg
     2341 ttcggtccct caccctcgcg atgcacagcc agagtcggat tcgcctgccg ctgcttcagc
     2401 tgaagacgtt cgatcaatac accaccaccc actcgaccaa cgtagctgtg ctcgccatgg
     2461 ccgttgccga gtacctgggg ctgggcgccc gtgagattcg gtcgttcggt gttgctggcc
     2521 tgctgcatga ccttggcaag gtgcgaatac cccgggacat cctggtaaag ccggggaagc
     2581 tgacggacga ggagcgcgcg gtcatgcaaa gccaccctgt cgatggcgcc cggatcatcc
     2641 tcgagcagga catgcaactc gacctcgccg cattcgtggc ctatgagcac cacatcatgc
     2701 tgaacggagg cgggtatccg acgctgcggt atcccaggga cgcgcactac gccagcaagg
     2761 tggtgcacgt ctgcgacgtc tacgacgccc tgtgcaccca tcggccgtac cgcgaagcct
     2821 gggcgtcgga gcgcgcactc ggatatctgg cggagcgggt gagtactgaa ttcgatcccg
     2881 acctggtcga cgcgttcacc cgcatgattg gtgactggga acaacggcgg agcccaattg
     2941 atgagaaaag cgtgaacggt gaacagtgaa cgggggctcc gtttctcagg ttgcccccgt
     3001 tcactgtcca ctgtttacgc agttacaaca tcaagactcc ctgccgcacg gcgacggcga
     3061 cggcttccgc tcgagtagac accccgagct tcgcgaacaa cgcggcgacg tggctcttca
     3121 cggtatgctc cgagataccc aggcgtgctc cgatcgtctt gttcgccaag ccggccgcca
     3181 gtagccccag aatttcaatc tcccgcgcag tgagcgtctg aggtacggcg tctgcggccc
     3241 gcggtgggcg ggctgcgttc ccgatcagct cgggcggcag cgcgaccagg ccgacggctg
     3301 cggcctcgac ggcggcaacg atctcagccg ggccggccgc gtgagacaac accgcccggg
     3361 caccgagccg gagcgcgcga gcgccccatt caaccactgg ctcgtctccg atcacgacca
     3421 gcggtggcgc cgatgtgccc tgatccgcag cgagctgggc gacagggcct gcatcgccgt
     3481 cggcgtcgag tgccaggaca atcacatcag gcgccgccgc gttctcagaa gtgggcaggc
     3541 cggggagacg ggcggagccc agcacggtgt aatcgccggc ccgggcgagc agtgtctcca
     3601 gcccggcccg tatcaccggc gagctggcca ccaccagaac ccgtctcaca ctcacgccgc
     3661 tcgcggtttc gccggcgggg tccccagaac gatctcgtgg gtgatccgtt cgaagccgcg
     3721 tatgatctca accttcagga ggtcgcctgg gcgagcctgc tccagcgcat cagccaggct
     3781 atccggatga ttgaaccgat caccgccgat ggccgtgagc acatcgccga tgatgatgcc
     3841 ggcgcggtca gcaccgctcc cgcgtgcgac ctggaggacg aggaagccaa cagcggcggc
     3901 ttggcggc
//
LOCUS       DASRJQ010000289         2481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1189071, whole genome shotgun sequence.
ACCESSION   DASRJQ010000289 DASRJQ010000000
VERSION     DASRJQ010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189071"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..2255)
                     /locus_tag="VFB89_06445"
     CDS             complement(78..2255)
                     /locus_tag="VFB89_06445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HZM26976.1"
                     /translation="MTHNFSSLPELPERAVRAARRFPWKLFLSLVVIAVFGSAGIVFG
                     VVQWLRRDLVSPQALTKIAAPVKTVVFDARGRVLHEFYKENRSPVPLKQIPRHLVNAT
                     ISTEDRSFYQHWGVDLWGVARAAVTDVLHMRRAQGGSTITQQLARNLLGTHERTFSRK
                     LKETVLAIEIERNFTKDQILEMYFNQIYFGEGAYGVESASKTYFNKSLRELTLAECAL
                     LAGTPANPSLYSPRRRPNAARARRDKVLRNMLTTRAITQVEFTNANAQPLGVTPARYS
                     NDRAPYFVEMVRLHLDEKYGSGAVYEGGLRVYTTLDMDLQQIAERALERQLLAIEAET
                     KPRNTLAKYAPATEAARLGQKTPYLQGSLVAIDPRTGYVRALIGGREWNHSNFNRATQ
                     ARRQPGSAFKPFVYVAAIDNGFHPTDVIVDEPVSFPGGNGELYQPMNYDREFRGPVTL
                     RYALQQSVNIPAIKLLRKVGTSLVASYARRMGIRTPIGQNLSLALGSSEVTLYELTTA
                     YGVFANRGIRNDPLFVLKVEDHTGTVLEKNSPRPVEVLSEQTASVMTSMLQSVMDHGT
                     GYPARALGFSIPAAGKTGTMDEYMDAWFVGYVPSLVCGVWVGYDEKRRIGGTMTGARA
                     ALPAWTEFMVGATRGRPADDFPLPAGTITQEVCAETGLLATTHCPNVTSEMFNEGSEP
                     NEPCHTHPGPLLSASRGASVSSNPRRATPQDTEKPPASALTPP"
BASE COUNT          434 a          755 c          837 g          455 t
ORIGIN      
        1 gaggccgccg cgtgctatgg gatttccgag acgcccgtcg acgccccacc gctcctgctc
       61 gaaaccctgg acagtcccta gggcggcgtc agcgccgagg caggcggttt ttcggtgtcc
      121 tgaggtgtgg cgcgccgcgg attgctcgag accgacgccc cgcgcgaggc gctgagcaat
      181 ggaccggggt gggtgtgaca gggttcattc ggctccgaac cttcgttgaa catctccgac
      241 gtgacgttcg ggcaatgcgt ggtcgccagc agtccggttt cggcacagac ctcctgggtg
      301 atggtcccgg caggcagtgg gaaatcgtcc gccggtcgac cccgcgtggc acccaccatg
      361 aactccgtcc aggccggcag agccgcccga gcgcccgtca tggtgcctcc gatccggcgc
      421 ttttcgtcgt agccgaccca cacgccgcag accaggctgg ggacgtaccc gacgaaccag
      481 gcgtccatgt actcgtccat cgtccctgtt tttcctgcgg ccggaatcga gaatccgagg
      541 gcgcgggccg gatacccggt cccgtggtcc atgacgctct gcagcatcga ggtcatgacc
      601 gaggcggtct gctccgagag cacctcgaca gggcgtggtg agttcttctc gagcaccgtt
      661 ccggtgtggt cttccacttt gagcacgaag agaggatcgt tgcggatccc ccgatttgcg
      721 aacaccccgt acgcggtcgt gagctcataa agcgtgacct cgctgctgcc cagtgcgagc
      781 gacaggttct ggccgatcgg agtgcggatt cccatgcggc gggcgtagct cgcgaccagc
      841 gaggtgccga ctttgcggag gagcttgatg gccggaatgt tcaccgactg ctggagggcg
      901 taacggagcg tgaccggccc gcgaaactcc cggtcgtagt tcattggctg atagagctcg
      961 ccattaccgc ctggaaacga gaccggctcg tccacaatca cgtcggtggg atggaatcca
     1021 ttgtcgatgg cggcgacata aacgaacggc ttgaaggccg agcccggctg gcgccgcgcc
     1081 tgggtcgcgc ggttgaagtt gctgtgattc cattcccggc cgccgatcaa cgcacggacg
     1141 taccctgtgc gagggtcgat ggcgaccaga gagccctgga ggtagggggt cttctggccc
     1201 aagcgcgcgg cttccgtggc aggggcgtac ttggccagag tattccgcgg tttggtttcc
     1261 gcttcgatcg cgagcagctg ccgctcgagc gctctctcgg cgatttgctg aaggtccata
     1321 tcgagcgtgg tgtagacgcg gagtccccct tcgtagaccg cgcccgagcc gtacttctcg
     1381 tccaggtgga ggcgaaccat ttccacgaag tacggagccc tgtcgttact gtagcgggcc
     1441 ggggtcacgc cgaggggctg cgcgttggcg ttagtgaatt ctacctgcgt gatggcgcgg
     1501 gtggtcagca tgttccgcag caccttgtct cgccgcgcgc gcgccgcatt gggtcgacgc
     1561 cgaggagagt agaggctcgg attggccggg gtgccggcca gcaaagcgca ctcggccagg
     1621 gtcagctctc gcagcgactt gttgaagtag gtctttgacg cggattcgac gccgtaagct
     1681 ccttccccga agtagatctg gttgaagtac atctccagaa tctggtcctt ggtgaagttg
     1741 cgctcgatct cgatggcgag gacggtttcc ttcagcttgc ggctgaaggt gcgctcatgg
     1801 gtcccgagga gattgcgcgc cagctgctgc gtgatcgtgc tgcccccctg ggcccgccgc
     1861 atgtggagga cgtcggtgac cgccgctcgg gccacgcccc agagatcgac gccccagtgc
     1921 tgatagaagc tccggtcctc cgtggagatc gtggcgttga ccaggtgacg ggggatctgc
     1981 ttgagcggca caggagagcg attctccttg tagaactcgt gcagcacgcg gccgcgggcg
     2041 tcgaagacga cagtcttgac gggtgccgcg atcttggtca gggcctgggg agagaccagg
     2101 tcgcgacgca gccactggac gaccccaaaa acgatcccgg ccgagccgaa aaccgcgatc
     2161 acgaccaggg acagaaagag cttccaggga aaacgccgcg cggcccgaac cgcgcgctcg
     2221 ggcagctcag ggagagacga gaagttgtga gtcacggcaa gaggctaggg tagaggtatg
     2281 gaaaagggca agcggattca ccctcctcaa cgtgacgagc ccgccggttg cccgtgtgcg
     2341 atctcgttcc tacaatgcaa tctgcagcga ccgtccgagt gtgcaaatgg agatcatcca
     2401 tgacgcccga gcaacaggtc cagatcttgt cacggaatac cgaggacgtg cttcctcacg
     2461 gagggctgct ggagcgactc c
//
LOCUS       DASRJQ010000290         3415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2535965, whole genome shotgun sequence.
ACCESSION   DASRJQ010000290 DASRJQ010000000
VERSION     DASRJQ010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2535965"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2055
                     /locus_tag="VFB89_06450"
     CDS             <1..2055
                     /locus_tag="VFB89_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZM26977.1"
                     /translation="AYYESPQALSFITSSQLREQAPAVVGDILSGLPGVDNSKDSPWE
                     QRPVLRGLGGQRVLVLMDGSPINSARGNGPHPSLVEPEQIERVEVVRGPSSVAYGSDA
                     LGGAINIITHEPQSGLVGPSLTGTAILGGSSVDGQRHAVLELMPKIGRLTSFISSGGR
                     TADDYSAASREVHNSAFKDYNALANLRYDFTDRTALKLGWQLYRGSDIGIPGLSFEEP
                     GALQEFDFAFYNRDYVHLTFDHSHRSAWLAGTRAKAYWQQERRNFFSNQEVQASRFND
                     PQFGLGPPPAGAELAITTQDRYYDIETGGFQAQLTSVRTVKTRFTAGIDAARDITDGD
                     NVRFRTWYDAAGNPLGATAERVSASIPDGRFDNYGTYAQAEWYAHRQWTVHGGGRYTH
                     YRYRTDYGLSAPAAGPNPPTYFQPLSIDDDALSGSLGLSFAARSDLHLTGHIANGYRQ
                     PNATELFFNGPASVGFVVGNPYLTPERSVSYDLGMRWGPSPFAISGNLFFSNYKDLID
                     ALPVVSGGPPGPPTFQYTNISDARIYGWEAETEWRFRRGWNARAQVSDAIGDITSAEA
                     IQTLYQVNQDTAPLPNVPPIHGTFSLRWSDDRGRFWVEPAARWAWRTSRLPLTLAGVP
                     QFSEFKAEWIVADLTTGARLPWGQKLLAGVRNVTDTPYRQALASVDDPGVNFFGQLTA
                     DF"
     gene            2331..2939
                     /locus_tag="VFB89_06455"
     CDS             2331..2939
                     /locus_tag="VFB89_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HZM26978.1"
                     /translation="MNSKSQAADTIASPLQEPRLPPGQVHTDKWPVLHYGSVPRVDLA
                     RWDFRVFGLVEQPVRWTYEEFLALPQVQVRSDIHCVTRWSRFDNLWQGVSFKEVLARV
                     QVRPEARFAVIHAEQGFTSNLALSELTQDDVLFAHRHDGQDLTPEHGWPLRLVVPRRY
                     FWKSAKWVRGVELVAEDRPGFWEQNGYHNEADPWREERFSDW"
     gene            2973..>3415
                     /locus_tag="VFB89_06460"
     CDS             2973..>3415
                     /locus_tag="VFB89_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26979.1"
                     /translation="MKEVPLHEWAKRLLEDQIERLGELRNANPRDHSFKLWRQTTLTV
                     IQRLWPGNLPKSEQFRRIAFSTPSARATRNQVRQHYERGCGEAVGYLRTLMMEIDAGA
                     LTRPTDTAALGAAISGLSPPVAPEASSEAFRDTRGLDEGLVGSGPL"
BASE COUNT          548 a         1225 c         1097 g          545 t
ORIGIN      
        1 gcctactacg agtcgccgca ggcgctgtcg ttcatcacct cctcgcagct gcgcgagcag
       61 gcgcccgcgg tggtgggcga tatactcagc ggcctgccgg gcgtggacaa cagcaaggac
      121 agcccgtggg agcagcgccc ggtgctgaga gggctgggcg gccagcgcgt gctggtgctc
      181 atggacggaa gcccgatcaa cagtgcgcgc ggcaacgggc cgcacccctc gctggtggag
      241 cccgagcaga tcgagcgcgt cgaggtggtg cgcgggccct cgtcggtggc gtacggctcg
      301 gacgcgctcg ggggcgcgat caacatcatc acccatgagc cacagtcggg cctcgtgggc
      361 cccagcctga cgggcaccgc catcctcggc ggcagctcgg tggacggcca gcgccacgcc
      421 gtgctcgagc tgatgccgaa gatcggccgt cttacgtcgt tcatctccag cggcggccgc
      481 accgccgacg actacagcgc cgcgagccgc gaggtccaca actcggcctt caaggactac
      541 aacgcgctgg ccaatctgcg ctacgacttc accgacagga ctgcgctcaa gctcggctgg
      601 cagctctatc gcggctccga tatcgggatt ccaggactgt cgttcgagga gcccggtgcg
      661 cttcaggagt tcgacttcgc tttctacaac cgcgactacg tccacctcac tttcgatcac
      721 tcgcatcgca gcgcctggct ggccggcacg cgcgccaagg cctactggca gcaggagcgc
      781 cgcaacttct tctccaacca ggaggtgcag gcgtcgcgct tcaacgaccc gcaattcgga
      841 ctcggcccac cgcccgcggg cgcggagttg gccatcacca cgcaggatcg ctactacgac
      901 atcgagaccg gaggctttca ggcccagctc acctcggtgc gcaccgtgaa gacccgcttc
      961 accgcgggca tcgacgcggc gcgcgacatc accgacggcg acaacgttcg gttccggacc
     1021 tggtacgacg ccgccggcaa cccgttgggt gcgaccgccg agcgcgtcag cgcctcgatc
     1081 cccgatggtc gtttcgacaa ctacggcacc tacgcccagg cggagtggta cgcacaccgg
     1141 cagtggaccg tgcatggcgg cggtcgttac acgcactacc gctaccgcac cgactacgga
     1201 ctctccgccc cggctgcggg cccgaacccg cccacgtact ttcagccgct gagcatcgac
     1261 gatgacgcgc tgtcgggatc gcttggcctc tcgttcgcgg cgcgcagcga cctgcacctg
     1321 accgggcaca tcgccaacgg ctaccgccag cccaacgcca cggagctatt cttcaacgga
     1381 cccgcctcgg tcggcttcgt ggtcggcaac ccctatctga ccccggagcg cagcgtttcc
     1441 tacgacctcg gcatgcgctg ggggcccagc ccgttcgcga tctcgggcaa cctgttcttc
     1501 tccaattaca aggacctgat cgacgcgctg ccggtcgtca gcggaggtcc ccccgggccg
     1561 cccaccttcc agtacaccaa catctccgat gcccggatct acggctggga ggccgagacc
     1621 gagtggcgtt tccggcgcgg ctggaacgcg cgcgcgcagg tgtcggacgc gatcggcgac
     1681 atcaccagtg ccgaagcgat ccagaccttg taccaagtga atcaggacac ggcgccgctt
     1741 cccaatgtgc cgccgatcca cggcacgttc tcgctgcgct ggagcgacga tcgcggccgc
     1801 ttctgggtgg agcccgccgc ccgctgggcc tggcgcacca gccgcctgcc gctgacgctg
     1861 gcgggggtgc cccagttcag cgagttcaag gcagagtgga tcgtggccga cctgaccacc
     1921 ggcgcccgac tgccctgggg acagaagctg ctggcgggcg tgcgcaacgt caccgacacg
     1981 ccgtaccggc aggctttggc ctcggtggac gatccggggg tgaacttctt cgggcaactg
     2041 acggcggact tttagacgcc ggctggttct agcgtcggcg ggcggtggcc gagccagggt
     2101 ctattcggcg gcccgttccg ccgacgctgt cctgcgtcgg ctccactgca tccactcgct
     2161 gctccgccat ccctggctgc gcggacgctc gtcggacgca ccgccgaatt gaccctggct
     2221 cggccccgcc cgccgactga gcgactctcc gaccagcgcc cgttttccca agccgccacg
     2281 cctgcggtac ggtgacgccc tccagaagcc ctgatccgag accaacaccc atgaattcca
     2341 agtcccaggc ggccgatacc atcgccagcc cgctccagga gccgcgactc ccgccgggcc
     2401 aggtacatac cgacaagtgg ccggtgcttc actacgggtc cgttccgcgg gtcgatctcg
     2461 ctcgctggga ttttcgcgtg ttcggactcg tcgagcagcc cgtgcgctgg acctatgagg
     2521 agtttctcgc gctgccccag gttcaggtgc ggagcgacat ccactgcgtg acccgctgga
     2581 gtcgcttcga caacctgtgg cagggcgtct cgttcaagga ggtgctcgca agagtccagg
     2641 tcaggcccga ggcgcgattc gctgtgattc acgccgagca gggcttcacc agtaacctgg
     2701 ccctttccga gctcactcag gatgacgttc tcttcgccca tcgccacgac ggccaggacc
     2761 tcactccgga acacggctgg cctttgcggc tcgtggtgcc gcggcgctac ttctggaaga
     2821 gcgccaagtg ggtgcgagga gtcgagctgg tggccgagga tcgccccggc ttctgggagc
     2881 agaacggcta ccacaacgaa gccgatccct ggcgcgagga acgcttctcc gactggtgag
     2941 gccccccttc gcacctcgcg aggggtgggg cgatgaagga agtcccgctt cacgaatggg
     3001 ccaagcgcct gctcgaggat cagatcgagc gcctcggcga gctgcgcaac gccaatcccc
     3061 gtgaccattc atttaagttg tggcgacaga cgacgctgac cgtcatccag cgcctgtggc
     3121 ccgggaacct gcccaagtcg gagcagttcc gccgcatcgc ctttagcacg ccttcggccc
     3181 gtgccacccg caatcaggtg cgccagcact acgagcgcgg ctgcggcgag gcggtgggct
     3241 atctgcgcac cttgatgatg gagatcgacg ccggcgcgct gacgcggccc accgacaccg
     3301 ccgcgctcgg ggccgccatc tcgggcctgt cgccgccggt ggctcccgag gcgtcctcag
     3361 aagcgttccg cgacacgcga gggctcgacg agggcctggt ggggagcggc ccact
//
LOCUS       DASRJQ010000291         3449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1897615, whole genome shotgun sequence.
ACCESSION   DASRJQ010000291 DASRJQ010000000
VERSION     DASRJQ010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1897615"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VFB89_06465"
     CDS             complement(<1..243)
                     /locus_tag="VFB89_06465"
                     /inference="COORDINATES: protein motif:HMM:NF013390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HZM26980.1"
                     /translation="MKETRREQAAVWARRLHDEITGFFTELDAGGAFREDVWERPGGG
                     GGVARVLAEGNTFEKAGINRSAVDGRLPAGLAQRLGS"
     gene            complement(240..1268)
                     /gene="hemE"
                     /locus_tag="VFB89_06470"
     CDS             complement(240..1268)
                     /gene="hemE"
                     /locus_tag="VFB89_06470"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HZM26981.1"
                     /translation="MNDLLLRACRREVVERPPVWMMRQAGRYLPEYRKLRERADFLTM
                     VGTPDLAVQVTLQPVDILGVDAAIIFSDILVVPQAMGMRLSVDEGVGPRFHQPLRTEA
                     EFARLRPLVPEESLGYVLEAIRLARRELEGRVPLIGFAGAPWTLASYMIEGGASRSFA
                     VAKRFLAEEPERAHALLGRLAKAVGAFLVTQVAAGAQVVQLFDSWAGALAPEDFRAFS
                     LPYLAETVRIAKSAGAPVIAFAPGAGWALEEIARVTGADVLGVDWQTEAAEANRRAAP
                     FPVALQGNLDPCWLYAPPPIIQQRTIAMLQAFSGRGHIANLGHGILPDVPVAHARMFV
                     ETVKTWRT"
     gene            complement(1285..2037)
                     /locus_tag="VFB89_06475"
     CDS             complement(1285..2037)
                     /locus_tag="VFB89_06475"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HZM26982.1"
                     /translation="MPRHSPTVVLTTAPGTLAGLADTLRAEGATVRETPLLSFGPPAD
                     WGPVDRAIFRLPEFGATAFTSPRAAETFASRLALHRVSPPPGQEAWATGAATAAALSS
                     LFDPVRTPSSLVTIDAGAGALLATAMIAAHVKPPVLYVCGDTRRDDVPTVLRAAGVRV
                     EEIVCYRSVVAEPDVMRRAAIGADVLLAASPKVVTMLARVCPKDERPALVVIGPTTAA
                     AARAAGWAPAGVARRPTVEAVIERIRGLVKGR"
     gene            complement(2037..3017)
                     /gene="hemC"
                     /locus_tag="VFB89_06480"
     CDS             complement(2037..3017)
                     /gene="hemC"
                     /locus_tag="VFB89_06480"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HZM26983.1"
                     /translation="MTTASGILRIGTRASALALWQTERVRAQLAQLGYATECVEIRTT
                     GDVVQDVPLSRLGGRAFFTKQLDDAMLEGRIDVAVHSLKDLPTELPEGIALAAVGQRE
                     DARDALVALKGLTWRTLPQGAVIATSSHRRRAQLLRARPDLELPDIRGNVDTRLKKLD
                     GHAEWSAIVLAAAGLVRLGLSGRISERLPFTVMLPAPGQGALAATVRADDVAAGEAVR
                     RALHDEDTAIAVSAERGLLQRLQGGCQVPVGAYATVDRQGDQAMVRLHARVVALNGAV
                     AVESVRSGSAATIEAAEMLGAALADELLGQGAAEILRSVRESAVQPISEP"
BASE COUNT          607 a         1190 c         1079 g          573 t
ORIGIN      
        1 agaacccagc cgctgcgcca aaccggccgg aagccggcca tccaccgccg accgattgat
       61 ccctgccttc tcgaaggtgt ttccttcggc gagtacccgg gcaactccgc ccccgccccc
      121 cggccgttcc cacacatcct cccgaaatgc gccgccagcg tcgagctcgg tgaaaaagcc
      181 cgtgatttcg tcgtgcagtc tccgcgccca gaccgctgcc tgctctcttc gggtctcttt
      241 cacgtccgcc atgtcttgac cgtttcgacg aacatgcgag cgtgggcaac gggaacgtca
      301 ggcagaatgc catggcccag gttggcaatg tgccctcggc cgctgaacgc ttgcagcatg
      361 gcgatcgtcc gctgctgaat gatcggagga ggcgcgtaga gccaacaggg gtcgagattc
      421 ccttgcagcg ccaccggaaa gggggcggcc cgacggttcg cttccgcggc ttcggtttgc
      481 cagtccacgc cgagcacgtc ggccccggtc acgcgagcga tttcctctag cgcccagcct
      541 gcgccgggtg cgaacgcgat aacgggtgct ccggcagatt tcgcaatccg aacggtctcg
      601 gccagatacg ggagagagaa cgcgcgaaag tcttccgggg ccagcgcccc cgcccaggag
      661 tcgaatagct ggaccacctg ggctcccgcg gcgacctgcg tcacgagaaa cgcgccgact
      721 gccttagcca accggcccag cagggcgtgt gcccgctcgg gctcttccgc caggaaacgt
      781 ttggcgacgg cgaagcttct ggaggcacct ccttcgatca tgtagctcgc gagagtccac
      841 ggcgcaccgg cgaatccgat cagcggtacc ctgccctcca gctcgcgtcg cgcgaggcga
      901 atggcctcga gcacgtaacc cagcgattcc tcgggcacca gcggtcgcag gcgggcgaac
      961 tcggcttcgg tacgcaacgg ttggtggaac cttggcccga cgccttcgtc gacggaaagc
     1021 cgcattccca tcgcctgcgg aactaccagg atgtcgctga agatgattgc cgcgtcgact
     1081 ccgaggatgt ccaccggctg aagcgtcacc tgcaccgcga ggtccggggt tcccaccatc
     1141 gtgaggaagt cggctcgctc ccggagcttg cgatactcgg ggagatagcg gccggcctgg
     1201 cgcatcatcc agaccggagg gcgctcgacc acctcgcggc ggcaggcgcg tagcaggagg
     1261 tcgttcattc gggtcaaggc tgagttagcg gcccttcacc aggccacgga ttcgctcgat
     1321 gacggcttcg acggtcggcc ttctcgcaac cccggctggc gcccatccgg ctgcccgggc
     1381 ggcagcggcc gtggtcggcc cgatcaccac gagggccggt ctctcgtcct tcggacatac
     1441 ccttgcgagc atcgtgacca ccttcggact cgcagccagc agcacgtccg ctccaatagc
     1501 ggcccgccgc attacatccg gctccgcgac tactgagcga tagcagacaa tctcttctac
     1561 ccggactcca gcagcacgca ggaccgtggg aacatcgtcc cgtcgggtat caccgcagac
     1621 gtagagcacg ggcggcttca cgtgcgctgc gatcattgcg gtggcgagca gcgcgcccgc
     1681 tcccgcatcg atggtgacca ggctcgacgg cgtacggacc ggatcgaaga gactactcag
     1741 tgccgcggcg gtggccgctc cggtagccca ggcttcctgg cccggaggtg gacttacccg
     1801 atgcagcgcg agacggctcg cgaaggtctc tgccgcacgt ggggatgtaa acgcggtggc
     1861 tccgaactcc gggagtctaa aaatcgcccg atcgaccggc ccccaatctg ccggcggccc
     1921 gaaagacagg agcggcgtct ctcgaaccgt cgcgccttca gctcgcagcg tatccgcgag
     1981 cccggctagc gttcccggcg ccgtcgtcag cacgaccgtg ggcgaatgac gaggcatcag
     2041 ggctcggaga tcggctggac ggcgctttcg cggacggagc gcaggatctc ggcggctccc
     2101 tgacccagca actcgtcggc caatgccgca cccagcatct cggcagcctc gatggtagca
     2161 gctgagccgc tccgcacact ctcgacggcc acggcgccat tcagggcgac cacgcgtgca
     2221 tgcagccgaa ccatcgcctg atctccctga cgatcgacgg tagcgtaggc ccccaccggt
     2281 acctgacagc caccctggag ccgctgcagc aatccgcgtt cggccgagac cgcgatggcc
     2341 gtgtcctcgt catggagcgc ccgacggacc gcctcgcccg cggccacgtc atcggcacgg
     2401 accgtagccg caagcgctcc ctggccagga gcgggcagca tcacggtgaa gggcagccgc
     2461 tcactgattc ggccgctcag accgagtctc acgaggcccg cggcagcgag cacgatcgcc
     2521 gaccactctg cgtggccgtc gagctttttc agacgcgtgt cgacattccc ccgaatatcc
     2581 ggcagctcga gatccgggcg ggcacgcaga agctgagccc gcctccggtg gctgctggtt
     2641 gcgattacgg cgccctgagg cagtgtcctc caggtaagcc ctttgagcgc cactaacgca
     2701 tcccgagcgt cttccctctg cccgactgcc gccagagcga ttccctccgg cagttcggtg
     2761 ggcaggtcct tgagtgagtg gactgccacg tcgattcggc cttccagcat tgcatcatcg
     2821 agctgcttcg tgaagaatgc tcgccctccc agtcgggaga gtggcacgtc ctgcacgaca
     2881 tcgccggtgg ttcgtatctc gacgcactcc gtggcatagc cgagctgcgc gagctgagct
     2941 cgcacgcgtt cagtctgcca cagcgcgagc gcactcgcgc gagttccgat tcggagtatc
     3001 ccggatgccg tcgtcactgt tccctttcac ggagcattag cgatccccta gaattctctg
     3061 gcttaggtgt gccaaatcta tatgtaagga ggtaacgcgg gccggaggcg aggtatgtct
     3121 tcttgcgaca gttacaagga gcgcgattcc cagcgagtcg aacgcgacgg gtcgggctgc
     3181 cgcgtatcca gaccgttgcg tctgcaagag gaccaccgtt gatcaggcta tcgcggagcg
     3241 tgccggacgc gctgagcctg agcggcggtt gccccaccga acactagggt gcacacagcc
     3301 gccgccaact gagccagaca atgccgactc cgaatcacag aatgcctccg ggtttttcag
     3361 ccgaatcggg gaaaatccct gatggatgcg gcccgaaggg gaggccattt ttcccacacg
     3421 gagaggcgca attgcgacaa cctgctcaa
//
LOCUS       DASRJQ010000292         4629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3684960, whole genome shotgun sequence.
ACCESSION   DASRJQ010000292 DASRJQ010000000
VERSION     DASRJQ010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3684960"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /gene="ribD"
                     /locus_tag="VFB89_06485"
     CDS             <1..1031
                     /gene="ribD"
                     /locus_tag="VFB89_06485"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HZM26984.1"
                     /translation="LGWGRVHPNPMVGAVVLAKGAPVAEGFHAEFGGPHAELQALAVA
                     GERAHGATLLSTLEPCNHSGKQPPCTEAILKAGIGRVVAALRDPNPIAAGGAERLRAA
                     GVEVDLGLMATEAARQNAAFLHLARHAARPYVALKLATSVDFRIADSSGRSRWVSGPQ
                     AREYVHWLRAGFDGIGVGLGTAVADDPRLSVRGAVAIRVPPVRIVFDRRLELPPHLDL
                     VRSVAQFRTLVVTAAGPPAERVDSIEKLGLTVLRTRDLDDALRQLRTAGVTALLVEGG
                     GRLAGDLLDAGLVDRFYWIQAPVWLGDGGIPAVRGLASAQLEEAARWQVVERRPLGDD
                     TLLVLDRT"
     gene            1031..1627
                     /locus_tag="VFB89_06490"
     CDS             1031..1627
                     /locus_tag="VFB89_06490"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HZM26985.1"
                     /translation="MFTGIITAVGKVIEARSENGGLDLVIEAPYDDLALGESVAVDGA
                     CLTVNSVGSGGFSVHLIATSLERTRFRRCRPGFRVNLERALRVGDRLGGHLVQGHVDG
                     VGEVVRVAQQDDARLLDIIAPAEIMGVSIPLGSITVDGVSLTINAIPAASIIQVSLIP
                     FTLQHTTLGERVAGHQVHLEGDTVGKYVAALLREKRER"
     gene            1712..2917
                     /locus_tag="VFB89_06495"
     CDS             1712..2917
                     /locus_tag="VFB89_06495"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HZM26986.1"
                     /translation="MTFGTIEQAVDDLKNGKLVIVADDEDRENEGDFVGAAELVTPET
                     INFMTLHGRGLICLALTGERCDQLGLPQMTERNTEDQATAFTISVDAERRFGVTTGIS
                     ASDRATTIHVAINPATVPTDLRRPGHIFPLRARPGGVLQRVGQTEASVDLARLAGLVP
                     AGVICEILNPDGSMARRPELETVAREHGLTFVTVAQLVAYRLRTERLVHRVAEARLPT
                     EFGEFCVIAYRNDVDKAEHVALVMGDVSGQPNVLVRMHSKCLTGDVFASQRCDCGFQL
                     HTAMQLVQREGRGVIVYLDQEGRGIGLLNKIRAYALQDSGDDTVQANQRLGFAPDLRN
                     YGIGAQILRDLGLSSIRVMTNNPRKLVGLEGYGLEIVERVPLVSSPTQDNLEYLRTKR
                     DKLGHLLAH"
     gene            2967..3407
                     /gene="ribH"
                     /locus_tag="VFB89_06500"
     CDS             2967..3407
                     /gene="ribH"
                     /locus_tag="VFB89_06500"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HZM26987.1"
                     /translation="MAILVSRYNEAVTARLLEGALACTDAAQIPRADVDVIWVAGAFE
                     LPVAAAASARTGRYACIVALGAVIRGETPHFEYVAEAATRGLGHVSVETGLPVGFGLL
                     TVETLEQAVERAGGSAGNKGYEAAAAAIEAADVIARLRASHAAD"
     gene            3394..3810
                     /gene="nusB"
                     /locus_tag="VFB89_06505"
     CDS             3394..3810
                     /gene="nusB"
                     /locus_tag="VFB89_06505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HZM26988.1"
                     /translation="MLRIETKARARALQLLYAWELQAGPSLPVIATGLARLSRCPDLD
                     VLERAERLAAGVVTRLAALDRHAAEAAENWRLSRIATVERNILRLGILELERADVPPK
                     VAIDEAVRLAHWFAGARAPAFVNGVLDRIAHSLGRL"
     gene            3807..>4629
                     /locus_tag="VFB89_06510"
     CDS             3807..>4629
                     /locus_tag="VFB89_06510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZM26989.1"
                     /translation="MNVLLVNWQDPENPYAGGAELHLFEIFKRLAAGGHRIRLICSGW
                     RGAVPSATIDGIEIRRISGRNGFALAGRGAVRRAIAEERPDIVVEDINKLPLFLPSLT
                     SLPFCAIVPHLFGATAYQEASWPAATVVWLGERPLARAYRRAGFHAISESTRDDLVAR
                     GVRAERIRVIHPGVDSDHFTPGPPGARASSPAFLYVGRLKRYKGVDVAIRALAVARRT
                     RADLRLEIAGSGDYASALRSLAERLGVGSAVTFHGFVSEAEKIRLLHQAWANVFPS"
BASE COUNT          746 a         1433 c         1568 g          882 t
ORIGIN      
        1 ggctgggctg gggccgcgtg catcccaacc ccatggtcgg tgccgtggtg ctggccaagg
       61 gagcgccggt ggccgagggg ttccatgccg agttcggcgg acctcatgcc gagttgcagg
      121 cgctggcggt ggcgggcgaa cgcgcacatg gcgcgacgct tctctccacg ctggagccct
      181 gcaaccatag tggaaagcag ccgccctgta ctgaggccat tctcaaggct ggcattgggc
      241 gtgtcgttgc cgcgctgagg gaccccaatc cgatagccgc gggtggcgcc gaacgcctgc
      301 gagcagctgg tgtagaggtg gacctggggc ttatggctac cgaggcagcc aggcagaatg
      361 cggctttcct ccatctcgcc cgccacgcag cgcgccccta tgtggcactc aagctggcca
      421 cgagtgtgga ttttcgtatt gccgatagct ccgggcgctc ccgctgggtt tccggtccgc
      481 aagcccggga gtacgtgcac tggctgcgag ccggcttcga cggaatcggc gtaggtcttg
      541 gcacagcggt ggcggacgat ccgcgtttga gcgtacgcgg agcagtggcg attcgggtgc
      601 cacccgttcg catcgtattc gaccggcggc tcgagctacc gccgcatctc gatctggtcc
      661 gcagcgtcgc gcagttccgc actctcgtcg tgaccgcggc tgggccgccg gctgagcgtg
      721 tcgactccat cgaaaagctg gggcttactg tcctgcgcac tcgcgacctg gacgatgcac
      781 tgcgtcagct gcgaaccgca ggggtcacgg cgttgttggt ggaaggtgga ggaagactcg
      841 ccggcgacct cctcgatgcc ggcctggtgg atcgctttta ctggattcag gcgccggtgt
      901 ggttgggcga cgggggcata ccggccgtgc gcggtctcgc atcggctcag ctggaagagg
      961 cggcccgttg gcaggtagtc gagcgccgtc cgttgggcga cgatacactg cttgttctgg
     1021 atcgaacctg atgtttaccg gcatcatcac ggcggtgggc aaggtcatcg aggcccgctc
     1081 ggagaacggt gggctcgacc tcgtgatcga agccccatac gacgaccttg ctctcggtga
     1141 gagtgtggcc gtggatggcg cctgtctgac cgtgaactct gtaggctccg gaggcttctc
     1201 cgtgcacctg atcgccacgt ctctcgagcg gacccgcttc cggcggtgcc ggccaggatt
     1261 tcgggtcaat ctggagcgtg cgttacgcgt aggtgatcga ctgggagggc acctggtgca
     1321 gggacatgtg gatggcgtgg gtgaggtcgt tcgagtggcc cagcaggatg atgcgcgctt
     1381 gctcgatatc atcgcccccg ctgaaatcat gggggtatcg attcccctcg gctcgatcac
     1441 cgtcgatggg gtaagtctta ccattaacgc cattcccgcc gcgtcaatca tccaggtatc
     1501 cctgattccg tttaccttac agcacaccac tctcggggag cgcgttgccg gccaccaagt
     1561 gcatctcgag ggggatacgg ttgggaagta tgtggcggcc cttctaaggg aaaagcgtga
     1621 acggtaaacg gtgaacggtg agcgggagga gccgtcgccg ggaacctccc gttcactgtt
     1681 taccgttcac cgctcacgca gtttcggggt tatgaccttc ggcactatag aacaagccgt
     1741 tgacgacctg aagaacggaa agctcgtcat cgtcgcggac gacgaagatc gcgagaacga
     1801 gggcgatttc gtcggcgcgg cagagctggt cacgcccgag accatcaact tcatgaccct
     1861 gcacgggcgg gggcttatct gtctcgccct gacgggcgaa cgctgcgatc agctcggcct
     1921 gccgcagatg acggagcgca acaccgagga tcaggcgacc gcgttcacca tcagcgtgga
     1981 tgcggagcgg cggttcggcg taaccacggg catctctgcc agcgatcggg caaccacaat
     2041 tcacgtcgcc atcaatcccg ccacggtccc gaccgatctt cgccgcccag ggcacatttt
     2101 ccccttgcgg gcgcgccctg gtggcgttct gcagcgggtg ggccagaccg aggcgagcgt
     2161 ggatctggcg cgcctcgccg ggctcgtccc ggccggcgtg atttgcgaga tcctgaatcc
     2221 tgacggctcg atggcgcgac gccctgagct ggaaacggtc gcgcgggagc atggtctcac
     2281 cttcgtcacg gtggcgcaac tggtggcgta tcggttgcgc accgagcgac tggtacaccg
     2341 ggtcgccgag gctcgactgc ccaccgagtt cggtgaattc tgtgttatcg cctatcgaaa
     2401 tgacgtggat aaggcggagc atgtggcgtt ggtgatgggt gacgtgtccg gtcagcccaa
     2461 cgtcctggtg cggatgcact cgaagtgtct caccggcgac gtcttcgcct cacaacgctg
     2521 cgactgcggt ttccagctgc acactgcgat gcagctggtg cagcgcgagg gccgtggggt
     2581 gatcgtgtat ctcgatcagg aaggccgcgg tatcggattg ctgaacaaga tccgggctta
     2641 cgctctgcag gactcgggtg acgacaccgt tcaggccaac caacggcttg gattcgcgcc
     2701 cgaccttcga aactacggca tcggcgctca gatcctgcgc gatcttggcc tctcttccat
     2761 tcgagtcatg accaacaacc ctcggaagct ggtgggcctg gaagggtacg gactggaaat
     2821 cgtagagcga gtaccgctgg tctcgagccc cacccaggac aacctggagt atctccgcac
     2881 taagcgcgac aagctcggcc atctcctggc gcactaaatt ccgttccatg cctgagctct
     2941 ccggccacct ccgacctgtc ggcagagtgg ccatccttgt cagccgctac aacgaggcgg
     3001 tcaccgcccg actgctcgag ggtgcgctcg cctgcaccga cgccgcgcag atcccgcggg
     3061 ccgacgtaga cgtcatctgg gttgcaggcg ccttcgagct cccggtcgcg gctgctgcct
     3121 cggcgcgaac cggacggtac gcctgcatcg tggcactcgg cgccgtgatc cggggtgaga
     3181 cgccgcattt cgagtacgtg gccgaagccg cgactcgtgg gctgggccat gtgtcggttg
     3241 aaaccgggct tcccgtcggc ttcggccttc tgacagtaga aacattggag caggcggtcg
     3301 agcgagccgg aggcagcgcg gggaacaagg gctacgaagc cgcggccgcc gcaatcgagg
     3361 cagccgacgt gatcgcccgg ttgcgcgcgt cgcatgctgc ggattgagac caaggcgcgt
     3421 gcccgcgcgc ttcagctcct gtacgcttgg gagctgcagg ccggtccgtc cctgcccgtc
     3481 atcgcaaccg gcctcgcgag actcagccga tgcccggacc tggacgtgct cgagcgcgcc
     3541 gagcggctgg cggcgggagt ggtcactcgt ttggcggcgc tcgatcgcca cgcggctgag
     3601 gcggcagaga actggcgcct gagtcggatt gctaccgtcg agcgcaacat tctccgcttg
     3661 ggcattttag agctcgagag ggccgatgtt cctcccaaag ttgccatcga tgaggccgtc
     3721 cgtctcgccc attggtttgc cggagctcgg gcaccggcgt tcgtgaatgg tgtgctcgat
     3781 cggatcgccc actcactcgg ccggctgtga acgtcctcct ggtcaactgg caggaccccg
     3841 aaaaccccta tgccgggggc gccgagttgc atttgttcga gatcttcaag cggttggcgg
     3901 ccggcggtca ccggattcgg ctgatctgct ccggctggcg cggcgcggtt ccaagcgcca
     3961 cgatcgacgg gatcgagatt cggcgcatct cgggtcggaa cgggtttgct ctggcgggac
     4021 gcggagcggt gcggagggca atcgccgagg agcggcccga catcgtcgta gaggatatca
     4081 acaagcttcc gcttttcctc ccgagcctca cctcccttcc attctgtgcc atcgtcccgc
     4141 acctgttcgg ggccacggcg tatcaggaag cgtcctggcc ggcggcgacg gtcgtttggc
     4201 tgggagagcg gccgctcgcg cgggcttatc ggcgagctgg gtttcacgcc atcagtgaaa
     4261 gtacccggga tgatctggtt gcccgcggcg ttcgtgccga gcggatccgg gtgatccatc
     4321 cgggggtcga tagcgatcac tttacgccgg gcccgccggg agcgcgggcg tcgtctccgg
     4381 cgttcttgta tgttggacgg ttgaagcgtt ataaaggagt ggacgtagcc attcgcgccc
     4441 tggccgtcgc acggcgcacc cgcgccgacc tgcgattgga aatcgccggc tccggtgact
     4501 acgcctcagc actgcggagt ctggccgagc ggctcggtgt cggctctgcg gtcacgtttc
     4561 atggatttgt gagcgaggcg gaaaaaattc gactgctgca tcaggcatgg gccaacgtgt
     4621 tcccatctc
//
LOCUS       DASRJQ010000293         3661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3558840, whole genome shotgun sequence.
ACCESSION   DASRJQ010000293 DASRJQ010000000
VERSION     DASRJQ010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3558840"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VFB89_06515"
     CDS             <1..691
                     /locus_tag="VFB89_06515"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZM26990.1"
                     /translation="EAALARAGAAVRAARLNLQALMGLSHPDTDFAVQGELHYQPVTI
                     PDSLVALAAANRPDLRAASMRVEQGRSLSSFAISELVPTPIVSLTYQNGTPFTNGHQY
                     QLGWGVSVPLFYWYGGERERAKAAKQAAGVEMRKTQTQLTADVAEALDAFRSSRLLAE
                     RYELGLLAKARAALETARYAYRAGATSQLDVLEAIRAYGDTRSEYYTAVRDYWVSVYA
                     LDRAVGKDIAP"
     gene            688..1842
                     /locus_tag="VFB89_06520"
     CDS             688..1842
                     /locus_tag="VFB89_06520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZM26991.1"
                     /translation="MTRLHSRSAWATGLLVALCLLIAMLLVACRTPDDAPAAELPAGT
                     HGEVRLAPNDPKLAYLTLDTVAPRTEKVVAVLPAQLVVDEDHTARIASPVTGRVRTID
                     VEPGDRVHAGQALAHISSSDVAQAESDLLKAQAALSQASTGLDRARDLYRNKVIALKD
                     LQQAESDAAQAYAEQERAAARVSLLGAAVDVVRQEFVLRSPIDGDVVERNLNPGAEVR
                     PDNAQTLFTISSLDTLWLTAGAYQRDLATAKKGDQLAFTTEASPGRRYLARVQYVSSV
                     LDPQTRTATIRAVLPNPDHTLRTQVFGEASLLAPDTARMPVVPVEALVTHGNQTVVYV
                     EATPGRFVRRPVEVGADDGRSAAITGGLQIGERVVCGGSLLLDAEAAGSR"
     gene            1843..>3661
                     /locus_tag="VFB89_06525"
     CDS             1843..>3661
                     /locus_tag="VFB89_06525"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZM26992.1"
                     /translation="MLRRLIRFALRQRVLIVGATVLLVIMGVRAFNDLPVEAFPDVED
                     VHVQVISQWPGHAAEEMETSVTLPVEREMNGLPYLTNLRSVSMFGLSVVTMTFVDGTT
                     DYFARQQVLERLQSVTVPPGVTPGLSSLSNSTGEIYRYTVSGNRPLTELKELQDWVVE
                     PAFRTVPGIADVVSFGGQVKQYQADLEPAQLRAYDVTLPEVEQAIAQANANTGGGYLA
                     HGYEKQVVRGVGLFTSVDEIASVSVASRGGVPIRVRDLGRVSVGGAPREGIVAKDTAD
                     DVVEGIVLMRKGENAVKVLNDVRAKTRELNQTGLPRGVQLVPFYDRAVLVNHTVHTVE
                     ENLSLGAGLVLVVLIVFLGDWRSAILVGLVIPLSLLGAFILMDLNHVSANLISLGAVD
                     FGIIVDAAVVMVEAFLVRLALAPPAPHEKRQLLAIIAESMGRPIAFAKAIVVTAFLPI
                     FTFQRVEKRIFSPMAFTLSFALVGSLILSLTLIPVLSSFWLKIKAHDNEPIAARWLRQ
                     AFSPCLDWVLGHRRLTLGLAIAALAVSLLLGSLLGTEFLPELDEGNIWLRVTMPVGIS
                     LEHAKEVERVVRRIVMSYPQVDQVVSHLGRPDDGTDAKGS"
BASE COUNT          627 a         1201 c         1191 g          642 t
ORIGIN      
        1 cgaggcggcc ctggcccggg caggcgccgc cgtacgcgcc gcgcgactca atctgcaggc
       61 gctcatgggg ctcagccatc cggacactga cttcgcggtg caaggcgagc tgcattacca
      121 accggtgacg attcccgatt cactcgtcgc cctcgccgca gcgaatcgcc ccgatctgag
      181 agcggcgagc atgcgggtgg agcaggggcg cagcctcagc tccttcgcca tttcggaact
      241 ggtaccgact ccaatcgtca gcctcactta ccagaacgga acgccgttca ccaacggcca
      301 tcagtaccag cttggctggg gagtctcggt gcctctcttc tattggtatg gaggcgaacg
      361 agagcgcgcc aaagcggcca agcaggcagc cggtgtggag atgcgaaaaa cacagacgca
      421 gctcaccgcc gacgttgccg aagcgctgga tgcattccgg tcgagccgcc tgctggctga
      481 gcggtacgag ttaggtctgc tcgccaaggc ccgcgccgcg ctcgaaacgg cgcggtatgc
      541 ctaccgcgcc ggtgcgacat cgcagctcga tgtgctcgag gcgattcgcg cgtacggcga
      601 cacccgaagc gagtactaca cggctgtgcg tgactactgg gtcagtgtgt acgcactcga
      661 ccgggccgtc gggaaggaca ttgctccatg acgcgtttgc actcccgcag tgcatgggcg
      721 acgggtctcc tcgtggcgct ctgtctcctg atcgcaatgc tgctggtcgc ctgccgcacg
      781 ccggacgatg cgccggcggc cgagcttccg gccggcaccc acggagaggt caggctcgcg
      841 cccaacgatc ccaagctcgc ctacctcacg ctggacaccg tggcgccacg gactgaaaag
      901 gttgtggccg tgctcccggc tcagctggtg gtggacgagg atcacacggc gcgcatcgct
      961 tcgcccgtca cgggccgcgt gcgcacgatc gacgtagagc cgggcgaccg ggtgcacgcg
     1021 gggcaggccc tggcccatat cagctcgagc gacgtggcgc aggcggaaag cgacctgctc
     1081 aaggcgcagg cagcactctc gcaggcgagc acaggactcg accgggcacg agacctttat
     1141 cggaacaagg tcatcgcgct caaagacctg cagcaggccg agagcgacgc cgcccaagcc
     1201 tacgccgagc aagaacgcgc tgcggcgcga gtcagcctgc tcggcgccgc ggttgacgtg
     1261 gtccgtcagg agttcgttct gcgctcaccc atcgacggtg atgtggtcga gcgcaatctg
     1321 aaccccggcg ccgaggtgcg acccgacaac gcgcaaaccc tctttacaat ttcgtcgctc
     1381 gataccttgt ggctcacggc cggcgcatac cagcgagatc tggccacggc caagaagggc
     1441 gaccagctcg ctttcaccac cgaggcgtcg cccgggaggc gctacctcgc gcgggtgcag
     1501 tacgtcagca gcgttctcga tccccagacc cgcacagcta ccatccgcgc cgtgctgccg
     1561 aatcccgatc acactctccg gactcaagta ttcggcgagg cgagcttgct cgcgccggac
     1621 accgcccgaa tgccggtcgt gccggtagag gcgctcgtga cgcacggaaa ccaaaccgtt
     1681 gtgtacgtcg aagccacccc gggccgattt gtgcggcgcc cggtcgaagt gggagctgac
     1741 gatggccgga gcgcggccat taccggcggc ctccagatcg gtgagcgggt ggtttgcggc
     1801 gggagccttc tcctcgacgc cgaagcggcc ggaagccgct agatgctccg ccggctcatc
     1861 cgctttgcgc tgaggcagcg cgttctaatc gtgggcgcga cggtgctcct cgtgatcatg
     1921 ggagtgcggg cattcaacga ccttccggtc gaggcttttc ctgacgtgga ggatgtccac
     1981 gtccaggtca tttcgcagtg gcccgggcat gcggcggagg aaatggagac ctcggtcacg
     2041 ctgccggtgg aacgcgagat gaacggcctc ccgtacctca ccaacctccg ctcggtctcg
     2101 atgttcggtc tgtcggtcgt gaccatgacc ttcgtcgacg gaacaaccga ctacttcgca
     2161 cgacagcagg tgctcgaacg attgcaatcg gtgaccgttc cacccggcgt tacccccggc
     2221 ctgagctccc tgtcgaattc gacgggcgag atctatcgct acacggtgag tggcaacagg
     2281 ccactgaccg agctgaagga gctccaggat tgggtggtag aacccgcgtt tcgaacggtt
     2341 cccggaatcg ccgatgtggt ctccttcggg ggacaggtaa agcagtacca ggcggacctg
     2401 gagccggcgc agctccgggc ctacgacgtc accctgccgg aggtcgagca ggcgatcgcg
     2461 caggccaacg ccaacaccgg cggtggctat ctcgctcacg ggtacgaaaa gcaggtcgtc
     2521 cggggagtgg ggctgttcac ctcagttgac gaaatcgcga gcgtatcggt cgcttcgcgg
     2581 gggggcgttc ccattcgcgt ccgtgacctg ggcagagtct ccgtcggggg tgccccgcgc
     2641 gagggcatcg tagccaagga cacggccgac gacgtggttg aaggcatcgt cctgatgcgc
     2701 aagggcgaga acgcggtcaa ggtcctcaac gacgttcgcg ccaagactcg ggagctcaat
     2761 cagacgggcc tcccccgagg ggtgcagctg gtgccgttct acgaccgcgc ggtcctggtg
     2821 aatcacaccg tgcatacggt ggaagagaat ctctcgctcg gggccggtct ggtgctggtc
     2881 gtgctcatcg tgttcctggg cgactggcga agcgcaatcc tcgtcggact ggtcattccg
     2941 ctctcgctgc ttggggcctt catcctgatg gatctgaacc acgtctcggc gaatctcatc
     3001 tcgctcggag ctgtggattt cgggatcatc gtggatgcgg cggtcgtcat ggtggaagca
     3061 ttcctggtgc ggctcgctct ggccccgccg gccccccacg agaagcgcca gctgctggcc
     3121 atcatcgccg agtcgatggg acggccgatc gcgttcgcca aggccatcgt ggttacggcg
     3181 ttcctcccta tcttcacctt ccaacgggtc gagaagcgga tcttctcgcc gatggccttc
     3241 acgctgagct tcgcgctcgt cggctcgctg atcctttcgc tcacgctcat cccggtgctc
     3301 tcgagcttct ggctcaagat caaggcgcac gacaacgagc cgattgccgc acgttggctc
     3361 cgccaggcgt tcagtccctg cctggactgg gtgctgggac accgccgcct gaccctcggc
     3421 ctggccatag ccgcgctcgc tgtctcactg ctgctgggaa gtctgctcgg caccgagttt
     3481 ctgcccgagc tagacgaggg aaacatctgg ctcagggtga cgatgccggt gggcatttcg
     3541 ctcgaacacg ccaaggaggt cgaacgcgtg gtccggcgga tcgtcatgag ctatccccag
     3601 gtcgatcagg tggtctccca ccttggccgg cccgatgatg gcaccgatgc gaaaggctcc
     3661 a
//
LOCUS       DASRJQ010000294         3189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_427420, whole genome shotgun sequence.
ACCESSION   DASRJQ010000294 DASRJQ010000000
VERSION     DASRJQ010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427420"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VFB89_06530"
     CDS             complement(<1..549)
                     /locus_tag="VFB89_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26993.1"
                     /translation="MEGTTTAPTEQPWIAAIRLSDSFRAAVADLAGELGAKLLEWTPT
                     GAERPPVGAALVLVLAGGEESAALELLPEIGRSAVPGFLVGASTDYRLATAAVQRGAR
                     DYFALPDDLELLRRSLERELREAGARRDAAQFADAERHATGFGAILGRSAELQRILSQ
                     AARVAGHGNVTVLIGGETGTGKE"
     gene            complement(521..1375)
                     /locus_tag="VFB89_06535"
     CDS             complement(521..1375)
                     /locus_tag="VFB89_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26994.1"
                     /translation="MLGVCLLAACSAENTGPGATLSQVRLGFASRAASTPSASAASLN
                     VSSPTPGTFTDGTNTLVLSKVQLVLRKIELRRVNATASACLDMAVSPDAGSGEAEPGD
                     DHGHDGEAEPGDDHGHDGVRDDCEEAEVGPVLFDVPVNSTGVKQSISILLDAGSYNAI
                     EFKIHKAEAPEDQPFLAANPSFAGKSIHVEGSWNGTNVTFDTDLDAEEELALQPPLTL
                     SADGAADVTILVDLHTWFADRSGGLVNPASGNRGQPNAGLVTENIQRSFHAFEDENED
                     GRDDHGTD"
     gene            1652..>3189
                     /locus_tag="VFB89_06540"
     CDS             1652..>3189
                     /locus_tag="VFB89_06540"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZM26995.1"
                     /translation="MRPHTLALGAFLLAAACSPDAGTARVGARTSAVVAAVGEPSSVL
                     PPLVTETVGRDIGDLVYERLADLRPGMSPIDATGYRPRLADRWERIDSRTWRFHLARG
                     ARWSDGRPVTSGDVKFSFDAFSDSLIDAPARPYLAGRVRIVPEDSSSFRVVFAERSPE
                     QLYDATYHVRVIPAHVWSAIPRESWAADTALGHLIGSGPYRVAEWRRGRFLRVQAREP
                     SKVGIGQVVWRFTPDPDAAINLLLGHEADLMELVGPAARDPRPAADSSLRLVSYPSAA
                     YGFLGFKIAGADGNQHSVLGSRATRRGLAMAVDRTALAHSVFGPSAVAPPGPMSRLLW
                     IWNDSIQTLPFDTARSAAELERAGWHRGRDGWRRRGSTVLKFDVLVPSTSSTRRELAT
                     ALQAMWRLVGARVTVTTVDFPVFQQRLREGKFDSYIGAWLDEPSPRGLADQWTRGGWA
                     GLNYGHYHDRDFDAAFNQAAATDDPVAAKKLYRRAMDALNADAPAIFLYSPTTTAAVS
                     KRLNG"
BASE COUNT          517 a         1042 c         1034 g          596 t
ORIGIN      
        1 ctccttcccg gtaccggtct cgcctccaat cagcacagtc acattcccat gccccgcgac
       61 gcgagctgcc tgggagagga tccgctgaag ctcggcgctg cggccgagga tggcgccgaa
      121 gccggtggcg tgccgttcgg cgtcggcaaa ctgagcagcg tcacgccggg cccccgcctc
      181 ccgcagctcg cgctccagcg agcgacgcaa cagctcgagg tcgtcaggca aggcgaagta
      241 gtcccgcgcc cctcgttgga ccgccgcggt cgccagccga tagtcggtcg acgcgccgac
      301 cagaaacccc ggcactgcag agcggccaat ctcgggaagg agctcgagag cagcgctttc
      361 ctcaccgcct gccagcacca ggaccaaggc ggcgccgacg ggtggcctct cggcccccgt
      421 cggcgtccat tcgagcaatt tcgcccccaa ctctcctgcg agatccgcca ccgcggctcg
      481 gaagctgtcg gaaaggcgaa ttgccgcgat ccagggttgc tcagtcggtg ccgtggtcgt
      541 cccttccatc ctcgttctcg tcctcgaatg cgtggaagga ccgctgaatg ttctcggtta
      601 ccagccctgc gttcggctgg ccccggttgc cgctggcggg attcaccagg cccccgctcc
      661 gatcagcgaa ccacgtatgc agatctacta gaatcgtgac gtccgctgcc ccgtcggcgc
      721 tgagcgtaag cggtggttgc agggcaagct cctcttcggc gtccagatca gtgtcgaagg
      781 tgacgttggt cccgttccac gatccttcca catgaatgct cttacccgcg aagcttggat
      841 tggcagcgag gaaaggctga tcttcgggag cctctgcctt gtggatcttg aactcgatcg
      901 cgttgtaaga accggcgtcg agcaggatcg agatcgactg ctttacaccg gtactattca
      961 ccggcacatc gaagagcacg gggccaacct cggcctcttc gcagtcgtcg cgtacgccgt
     1021 cgtgcccgtg gtcgtcgccc ggctcggcct cgccgtcatg gccgtgatcg tcgcctggct
     1081 cagcctcgcc agatcctgcg tccggtgaaa ccgccatgtc gaggcatgcc gaggccgttg
     1141 cgttgacacg gcgaagctcg atcttgcgaa gcaccagctg gactttgctc agcaccagcg
     1201 tgttggtccc gtcggtgaac gtccccggcg tgggcgaact aacgttcagc gatgccgcgc
     1261 tggcgctggg cgtgctggcg gcgcgggagg cgaatcccaa gcggacttgg ctgagcgttg
     1321 cgccggggcc ggtgttttcg gcactgcagg cggcgaggag gcagactccc agcaatagag
     1381 ggctccgcct gaataagctg caggacatcg gttgctccga gtgatgggta cgatgtgcgg
     1441 tcgtggtgac tcggagcccc acaaggcaag ggcggtaccc atcgatgccg tgcagttaac
     1501 atattgctga cgaatcattt agaagtctgg gagagagtac agcgccggcc tgcccgcaac
     1561 gtaatatcac atgctgtaac ggaatgttgc agatcgctgg ctgctcggca caggccagta
     1621 acctccaaca gggaacttct ggaaccgccc tatgcgaccg cacacactcg cccttggagc
     1681 cttcctgctg gccgcggcgt gctcgccgga cgccgggacc gcccgcgtcg gagcccgcac
     1741 ttccgccgtg gtcgccgcgg tcggcgagcc gtcgagcgtg cttccgccgc tggtgacgga
     1801 gacagtcggt cgggacatcg gggatctggt ttacgagcgg ctcgcggacc tgcgtcctgg
     1861 catgtcaccc atcgacgcca cgggatatcg gcccagactc gcagaccgtt gggagcgaat
     1921 cgactcgcgg acctggcgat tccatctggc tcggggagct cgttggtcgg acgggcgtcc
     1981 ggtcacaagt ggggacgtga aattctcctt cgatgccttc agcgactccc tcatcgatgc
     2041 gcccgcccgg ccttatctcg ctggccgggt gcggatcgtt ccggaagatt ccagctcgtt
     2101 ccgcgtcgta tttgctgagc gttcgcctga gcagttgtac gacgccacgt atcatgtgcg
     2161 ggtcataccg gcgcacgtgt ggagcgcgat tccgcgagaa tcctgggcgg ccgacactgc
     2221 gctcggtcat ctgattggct cgggtccgta tcgggtagcg gaatggagac ggggtcgctt
     2281 cctcagagtc caggcacggg agccgtcaaa ggtcgggatc gggcaggtgg tttggcgttt
     2341 tacccccgat cccgacgccg cgatcaatct cctgttgggc catgaagcgg atttgatgga
     2401 gctagtgggt ccagctgcgc gagatcctcg tccggcggcc gactcaagtc ttcggctggt
     2461 gtcgtacccc tccgccgctt acggctttct gggtttcaag atcgcgggcg cggacgggaa
     2521 ccagcattcg gtcctgggca gccgtgccac ccgtcgcggc ctcgccatgg ccgtcgatcg
     2581 caccgcgctg gcgcattcgg ttttcggccc cagcgccgtg gcgcctcctg gcccaatgtc
     2641 tcggcttctc tggatctgga atgacagcat ccaaacgctc ccgttcgaca cggcgcgatc
     2701 agcggcggag ctggagcgcg ccggctggca ccgagggcgc gatggttggc ggcgcagggg
     2761 aagcactgtc ctcaagttcg acgtgctcgt gccttcaacc agctcgactc gtcgcgagct
     2821 tgcgaccgcg cttcaggcga tgtggcgtct ggtcggtgcg agggtcaccg tgacgaccgt
     2881 cgacttcccg gtctttcagc aacgtcttcg cgagggcaag ttcgacagtt acatcggtgc
     2941 gtggctggat gagccaagcc cccgcggcct ggcggaccag tggacccggg gcggatgggc
     3001 tggactcaac tacggccact atcacgaccg cgacttcgac gcggcgttca atcaggcggc
     3061 tgccaccgat gatcccgtgg ccgcgaagaa gctgtatcgc cgagcaatgg atgcgctcaa
     3121 tgcggacgca cccgccatct tcctctactc gccaaccact acggcggctg tgagcaagcg
     3181 cctcaacgg
//
LOCUS       DASRJQ010000295         5543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2617924, whole genome shotgun sequence.
ACCESSION   DASRJQ010000295 DASRJQ010000000
VERSION     DASRJQ010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2617924"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VFB89_06545"
     CDS             complement(<1..589)
                     /locus_tag="VFB89_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM26996.1"
                     /translation="MSSSLVGPAVVLCLASLSPELLWGQTDARLAQGVRLAQEGLGDS
                     ARTVVQRVVDATNPADTLYPQALYTRAVVGANPQDIRRDLQRIAVEYPNSSWADDALL
                     RLAQLDYAARDLDAASRDLERLRLDYASSQLYPQASFWAARVYFDSRRPSAACRWVAE
                     GRPRVGKDVELRNQLDYYKDRCVGVALDTASADTSG"
     gene            complement(586..1647)
                     /gene="holA"
                     /locus_tag="VFB89_06550"
     CDS             complement(586..1647)
                     /gene="holA"
                     /locus_tag="VFB89_06550"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HZM26997.1"
                     /translation="MPSQTIDTLLRSLKRGEIVPAYYFFGSEDLLKEEAIRSLLDQVL
                     DPGLRDFNLDQRSAAQADPEEVYALCRTPAMMAERRVVILRDVEAWKRKTKARNVALD
                     YLAKPSPDTVLVLVQGSADEREDKELARLAVTVHCDPLPPERAARWAHRHAKKLDIEL
                     APGAADLLVRTVGVSLGALTAEVEKLAAAGDGTPVSEERVAALVGIRHGETMFDWRNA
                     VFDSQAARAAGMLDIVLDQAGVTGVKLLTLIGATLIGIGATRAAYDRNVRGSRLEDSA
                     FQLMLRARPSALAGVSYKEESRLWARWSASWPLERIRAGLRQSVIADTALKNTTVSDE
                     RGVLLDLVYGLAMPMSEVA"
     gene            complement(1683..2225)
                     /locus_tag="VFB89_06555"
     CDS             complement(1683..2225)
                     /locus_tag="VFB89_06555"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZM26998.1"
                     /translation="MSLLSSRRLYTGRVLNLDLDTVRFPDGSVGELEMIRHPGASAVV
                     PFVDDPQGNNPGVIMIRQFRHAADSYVWEIPAGRLEPGEAPEACARRELEEEAGMRAA
                     SLERLTTIFTTPGFTDERIHLFLATGLTEGTARREADEFVEVRELPWSEIMGLARQGE
                     IADGKTLISLLFVESFRRGT"
     gene            complement(2275..2922)
                     /locus_tag="VFB89_06560"
     CDS             complement(2275..2922)
                     /locus_tag="VFB89_06560"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HZM26999.1"
                     /translation="MKASTSNSSAPQRLKPLIGWHLLVVALLVLVGMIYTVAFVLVLI
                     ASQLDERQNADAIVVLGAAQYNGWPSPVLRARLDHALALYHERLAPMIVVTGGVGRRD
                     KESEATVARRYLLSHDVPDSAVVMQPQGRSTQASMTAVGNWLASRGRHSAILVSDPFH
                     MLRLRLEARRTGFTAYTSPTETSPISDNPVLELEYLAAEAVKVPVAWARSLEWFH"
     gene            complement(2903..4294)
                     /locus_tag="VFB89_06565"
     CDS             complement(2903..4294)
                     /locus_tag="VFB89_06565"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZM27000.1"
                     /translation="MPGIRLTFWGAAGQVTGSMHLLEAAGARVLLDGGLFQGHRTEAH
                     DLNANLQFDPRRIDAIILTHAHIDHSGRLPLLVARGFHGPIYATPATRDLSAVMLLDA
                     AHIQEKDLEFLERRGKAGPASEPLYTRADAVAVQDLTIGLPYRRLLHLRKNLSAEFTD
                     AGHILGSASLDLRLSEPAPHRLVFSGDIGRSGLPIVRDPVPPEGPIDTLIVESTYADR
                     VHESVADAETRLGEVIRRVAGRGGKVLVPAFALGRAQELIYALHRLWRNKAIPEIPVY
                     VDSPLAVDVTAVFRLHPEIFDATERLVGNGTQLFDFPLVRYVKDVADSKKLNTLTGPA
                     VIVAASGMAESGRILHHLANHAGDHRNCILFAGFQAENTLGRRIQDGADKIRIFGQEY
                     ERRAEIVSLSGYSAHADRNELRDWVRRLGGPIRRAFVVHGEPPALQAMATILKEEGVR
                     EAVIPKHGESFDL"
     gene            complement(4302..4946)
                     /gene="upp"
                     /locus_tag="VFB89_06570"
     CDS             complement(4302..4946)
                     /gene="upp"
                     /locus_tag="VFB89_06570"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HZM27001.1"
                     /translation="MPEPGHPNLTILSHPLIQHKLTLLRDKRTSTRDFKQLVSEIAML
                     MAYEVTKDLPTQPVEIETPLERTTGRQVSGKKLTLVPILRAGLGMVDGVAQLVPSARV
                     GHIGLSRDEATLRPTDYYFKIPGDEEARDFFVLDPMLATGGSGVSAVKALKQAGARRI
                     RFLCLVAAPEGALAMERAHPDIPVFAAALDRGLNDRGFILPGLGDAGDRLFGTR"
     gene            complement(5059..5424)
                     /locus_tag="VFB89_06575"
     CDS             complement(5059..5424)
                     /locus_tag="VFB89_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27002.1"
                     /translation="MPNWAVGLVLAAVFTAPCSSIAAQSNDRPLAVRIETGDSTTRLV
                     LVAAPGWKINARLKPALELSGGEIIRFDAPLLTPDSAYFAEPPSARLPGRTTAIHGTL
                     KASACAAGERVCRLLVLRL"
BASE COUNT         1066 a         1700 c         1839 g          938 t
ORIGIN      
        1 tgccgctcgt atccgctgat gcggtgtcga gggccacgcc gacgcaccgg tccttgtaat
       61 agtccaattg gttgcgcagc tcgacatctt tgccgactcg gggccggcct tccgcgaccc
      121 accggcaggc agcggaaggc ctacgcgaat cgaagtagac gcgcgctgcc cagaacgagg
      181 cctgggggta gagctgggaa cttgcatagt cgaggcggag gcgctccagg tcacgcgaag
      241 cggcgtccag gtcacgcgca gcatagtcga gctgagccag acgcaggagc gcatcatccg
      301 cccagctcga gttgggatat tcaaccgcga ttcgctggag gtcccggcga atgtcctgcg
      361 gattggcccc aacgacggcc cgggtgtaga gcgcctgggg atagagcgtg tccgccggat
      421 tggtagcgtc aaccacacgc tgaaccacag tgcgagccga gtcgcccaag ccctcctgag
      481 cgaggcgcac gccctgagcc aggcgagcat cggtctgtcc ccaaagcagc tcgggggaca
      541 gcgaggcgag gcaaagcacg acagcggggc cgaccagact actgctcatg ccacctccga
      601 cattggcatc gccaggccgt agaccagatc cagcaagacg ccgcgctcgt cggagaccgt
      661 ggtgttcttg agcgctgtgt cggcaatgac gctctggcgc agtccggcgc ggatccgctc
      721 gagcggccag ctcgccgacc agcgggccca gagtcgagat tcttccttgt agctgacgcc
      781 ggccagggcg gacggccttg cccgaagcat caactggaaa gcggaatcct cgaggcgaga
      841 tccgcgcaca ttccggtcgt acgcggcacg ggtggcgccg ataccgatca gcgttgctcc
      901 aatcaaggtg agcagcttga cgcccgtcac tccagcctga tccaggacga tatcgagcat
      961 gccggccgcg cgggccgcct gactgtcgaa gacggcgtta cgccagtcga acatggtctc
     1021 gccgtgccgg atacccacca gtgcagccac acgttcttcc gaaacgggcg tcccatctcc
     1081 tgcggccgca agcttctcca cctcggcggt caacgcgccg aggcttacgc caacagttcg
     1141 caccagcagg tcggcggcgc ccggcgcgag ctcgatgtcg agctttttgg cgtgccggtg
     1201 cgcccatcgg gcggcacgct ccggaggaag cggatcgcag tgaactgtta ccgccaagcg
     1261 tgccagctcc ttgtcttccc tttcatccgc cgagccttgc accagaacca gaacggtgtc
     1321 cggggatggc ttggccagat agtcgagcgc aacattgcgg gccttggtct tccgcttcca
     1381 ggcctccacg tcgcgcaaaa tcacgacccg tcgctccgcc atcatggccg gcgtacggca
     1441 aagggcatac acctcttcgg gatcggcctg ggcggccgag cgttgatcga gattgaagtc
     1501 gcgaagtcca gggtcgagca cctggtcaag cagagaacgg atcgcctctt ccttgaggag
     1561 atcctcgctg ccgaaaaagt agtaggcagg cacaatttcg ccccgcttca gagagcggag
     1621 caacgtatcg atcgtctgag acggcatgct gccgaaagat acgaggtggg ttcggccgcc
     1681 cgctacgtcc cgcgccggaa cgattcgaca aaaagcagcg agatcagcgt cttcccatcg
     1741 gcgatttcac cctgccgcgc aaggcccatg atttcggacc aggggagctc ccggacctcg
     1801 acgaattcgt ccgcttctcg ccgagcagtg ccctcggtga gtccggtagc gagaaacagg
     1861 tggattcgct cgtcggtaaa gcccggcgta gtaaagattg tggtcagacg ttcgagtgac
     1921 gcggcgcgca tgcccgcttc ttcttccagc tcccgcctgg cgcaagcctc gggtgcctcg
     1981 ccgggctcga gtcgacctgc cggtatttcc cacacatagc tgtcggccgc gtggcgaaac
     2041 tggcggatca taatcacccc gggattattg ccctgtggat cgtcgacgaa agggacgact
     2101 gcagacgctc ccggatggcg tatcatctcc agctcgccca ctgagccgtc gggaaagcga
     2161 acggtatcga gatcgaggtt gagaacgcgg ccggtgtaga gacgacggga ggataggagg
     2221 gacataaaca agcaggagaa aggggaaagg gagaaagggt gaagggggaa ggggctagtg
     2281 gaaccactcg aggctccgcg cccaggcgac tggaaccttg actgcctcgg cggcgagata
     2341 ctcgagctcc agcactgggt tgtcggatat cggactggtc tcggtgggtg aggtgtaggc
     2401 ggtgaacccc gtgcgccggg cctcgaggcg aaggcgcaac atgtgaaacg gatcgctcac
     2461 cagaatcgcg ctgtggcggc cgcggcttgc gagccaattg ccgacggccg tcatcgaggc
     2521 ttgagtggag cgtccctgag gctgcataac gacggctgag tcaggcacat catgggagag
     2581 gaggtaccgt cgggcaacgg ttgcctcact ttccttgtct cggcgcccca ccccgccggt
     2641 cacgacgatc atcggggcga gccgctcgtg gtatagtgcg agcgcgtgat ccagccgagc
     2701 gcgcaggacc ggggagggcc aaccgttgta ctgcgcggct ccgagaacga cgatcgcgtc
     2761 agcattctgg cgctcgtcca gctgggacgc aatcagcacc agcacgaacg caaccgtgta
     2821 gatcattccc accagcacca agagtgcgac gacgagcaga tgccaaccga tcaacggctt
     2881 gagccgttgc ggcgcgctgc tgttagaggt cgaagctttc accgtgcttg gggatgactg
     2941 cctcgcgtac gccttcctct ttcaagatgg ttgccatggc ctggagcgcc gggggctcgc
     3001 cgtgcacgac gaaagcacgt cgaatcggac caccgaggcg ccgcacccaa tcccgcagct
     3061 cgttgcggtc ggcatgggcc gagtagccgc tcaggctcac tatctcagct cggcgctcgt
     3121 actcctgccc gaagatcctg atcttgtctg cgccgtcctg gattcgtcgc cccaatgtgt
     3181 tctcggcctg aaagcccgcg aacaggatgc agttccggtg gtccccggcg tggttcgcga
     3241 ggtggtgaag aatcctgccg gactccgcca ttcccgacgc tgcgacgatg actgcgggtc
     3301 cggtcagcgt gttgagcttc ttcgagtcgg ccacatcctt gacgtaacgg accaggggaa
     3361 agtcgaagag ctgggtacca ttccccacca accgctcagt cgcatcgaag atctcgggat
     3421 ggagccggaa cacagccgtc acgtctaccg cgagcgggct gtcgacgtag acaggaatct
     3481 ccggaatcgc cttgttccgc cagagccggt gcaacgcgta gatcagttcc tgggcccggc
     3541 ccagcgcgaa cgcgggaacc agaacctttc ctccccggcc cgccaccctg cgaattactt
     3601 cgcccaaccg ggtttcggca tcggcaacgg actcatggac ccggtcggcg taggtggact
     3661 cgacgatcag ggtatcaatc gggccttccg gcggcaccgg gtccctgacg atcggaagac
     3721 cgctccttcc gatgtcacct gaaaagacca gccgatgtgg tgcgggctcg gacagccgaa
     3781 ggtcgagcga ggccgagcca agtatgtgac ccgcatcggt gaactcggcc gacagattct
     3841 tgcggaggtg cagcaggcgc cggtacggta ggccgatcgt gaggtcctgc actgcgaccg
     3901 cgtcggcccg ggtgtagagc ggttcgctgg cgggccccgc cttgccgcgt cgttcgagaa
     3961 actcgaggtc tttttcctgg atgtgcgcgg cgtcgagcag catgactgcc gaaagatccc
     4021 gagtggcggg cgtcgcatag atcggtccgt gaaacccccg cgcgaccagc agcggcaatc
     4081 gaccgctgtg atcgatgtgg gcgtgggtca ggatgatcgc atcgatccgg cggggatcga
     4141 attgcagatt ggcattcaga tcgtgcgcct cggtccgatg accctggaac aagcctccgt
     4201 cgagcagcac acgcgcgccc gctgcctcga ggagatgcat ggatccagtg acctggccgg
     4261 ctgctcccca gaaggtcagt ctgattccag gcatcgtttg gtcagcgagt cccgaaaagg
     4321 cgatcgccgg cgtcgccgag accggggagg atgaatccgc ggtcgttgag gccgcgatcg
     4381 agcgctgcag cgaacacggg aatatcaggg tgcgcacgct ccatggcgag cgcaccctca
     4441 ggggcggcga ccaggcagag aaaccggatg cgcctcgcgc ctgcctgctt gagcgctttc
     4501 acggcggata caccggatcc gccggtggcg agcatcgggt ccagcacaaa gaaatcccgg
     4561 gcctcttcgt cacccggaat cttgaagtag tagtcggtgg gtcggagcgt cgcttcgtcg
     4621 cgcgagagtc cgatgtggcc cacgcgggcg gaggggacca attgggcgac accgtccacc
     4681 atgccaagcc ccgcgcggag aatggggacc agagtgagct tctttccgga gacctggcgc
     4741 ccggtcgttc gttcgagcgg ggtctcgatt tccaccggct gggtgggaag gtccttggtg
     4801 acctcgtaag ccatcaacat cgcgatctcg ctcacgagct gcttgaagtc gcgagtgctg
     4861 gtccgcttgt cgcggagtag agtcaatttg tgttgaatga gggggtgtga gaggatcgtg
     4921 aggttcgggt ggccgggctc tggcatgcga cggaagatac tgcgtaagcg gtgaaagggg
     4981 gaaaagcgga agggcgggcg aacgggaaaa agggggaagg ggagaaagga gaaagggaga
     5041 gagggaaaga gagagcagtt agagtcgtaa cacgagcagt cggcacactc gctcgccagc
     5101 cgcgcatgcg ctcgctttga gagtgccgtg aatcgctgtg gtccgaccgg gaagacgggc
     5161 cgaaggcggc tcggcaaagt aggcggaatc gggagtgagg agcggggcat cgaagcgaat
     5221 gatttcgcca ccagatagct caagagccgg cttgagcctg gcgttgattt tccagcccgg
     5281 cgccgccacc agaacaagcc gcgtcgtgga gtccccggtt tcgatccgaa cagcgagggg
     5341 gcggtcgtta ctttgtgccg cgatcgacga gcagggcgcg gtgaagaccg ccgccagcac
     5401 cagcccgacc gcccaattcg gcacgcgcct cattgttcgt tcagcagctc gcggatccgt
     5461 ccgtcccaca gtcgatatgt cgcatcaccg acatggaact ctcccgcgcc ctcgtaacgg
     5521 atgcgccctt tcttgtcgat gag
//
LOCUS       DASRJQ010000296         9682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_330963, whole genome shotgun sequence.
ACCESSION   DASRJQ010000296 DASRJQ010000000
VERSION     DASRJQ010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 9682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9682
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330963"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VFB89_06580"
     CDS             complement(<1..579)
                     /locus_tag="VFB89_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZM27003.1"
                     /translation="MPGETLIVLSDAHLGYAPAAVETALLEFLDIIPSLGDALLVNGD
                     LFEFWFAYRTVVPRHAFRTAAALAALARKVPILMVGGNHDRWGLEFWNREAGILYAPH
                     RLEFEAGGRRGLAIHGDGLGSSRPLTRVMERLVASRAASVVYRSLHPELGFPLVHRLS
                     AMLGEHGLHGPEVDSAAARQRVWAERQLAAQSS"
     gene            604..1044
                     /locus_tag="VFB89_06585"
     CDS             604..1044
                     /locus_tag="VFB89_06585"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZM27004.1"
                     /translation="MTGQPGQHRVCEIQVRVNYSEIDQMGVVYHARHLVWLDIARCEY
                     LRQTGLSYRELEASGLRLVVGEVAIRYRQPARYDDLLRVRCWVRDVASRRVEFGYAVE
                     HAENQRLIATARVALHPLDEQMALCRLPDDLSRRLTPIPDPVRL"
     gene            1116..1826
                     /locus_tag="VFB89_06590"
     CDS             1116..1826
                     /locus_tag="VFB89_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27005.1"
                     /translation="MDQPFHLIRVVATAGLVAAAGCHTAPGPLLPAAAAPVSPEQVTG
                     WVNATVPTDHRLLRFKWLFRDERSSAGGRGSARIAPPDSLRFDVAGPFGSNPAAAAVV
                     GDSAVWVRPEDVIKKLVPNYPLMWALLGVARQPDSGAVLRGLTEGQTTMWQYASGTDT
                     VEYVRTSESPGKLLAEVRQGSKVVGRVETQFGADGSPASARLTVPSVPARLDLTFLSI
                     ARNSSFAPTTWQPPPPKP"
     gene            1897..2289
                     /gene="lptE"
                     /locus_tag="VFB89_06595"
     CDS             1897..2289
                     /gene="lptE"
                     /locus_tag="VFB89_06595"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HZM27006.1"
                     /translation="MLPFDNLTPEPTLTQEVQNAVRNAVQGRLGLRQSGEQSADVIVR
                     GSIQRYEPDLPVAYQGVQNNQVDVTRRLVQITVNVEITNQRTGKILYQRSGLTLQGDY
                     ATGQEPSGRQKAYDQLVTNIVEGAQSQW"
     gene            2283..2654
                     /locus_tag="VFB89_06600"
     CDS             2283..2654
                     /locus_tag="VFB89_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27007.1"
                     /translation="MVSSSRIDRRGTSTLGCLVSLLVFVAALYYGIHIGEVYWRYYQI
                     LDEMKSNARVAPNLADDVIQRRLQAKVQDVFGDEVQMKFRINRGGRSRTITIDTEYRD
                     SVDLPLFKRGFELKPHVEEPI"
     gene            2670..3380
                     /gene="rph"
                     /locus_tag="VFB89_06605"
     CDS             2670..3380
                     /gene="rph"
                     /locus_tag="VFB89_06605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HZM27008.1"
                     /translation="MARPDGRSSEQLRPLVLERAANPYAEGSCLIRMGGTLVHCTATV
                     EPGVPGFKKGTGEGWVTAEYAMLPRATLERTPRERNGPGGRTQEIQRLIGRSLRACMS
                     TMAFGEFTIRVDCDVLQADGGTRTASITGGCVALADACQWLADKTKIASPFGRLVSAI
                     SVGIVEREIRVDLAYLEDRDAGVDANVVMLEPAQFVEVQGTGEHGTFSRSELGTLLDL
                     AERSCAELFAAQRRTLGW"
     gene            3377..3991
                     /locus_tag="VFB89_06610"
     CDS             3377..3991
                     /locus_tag="VFB89_06610"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HZM27009.1"
                     /translation="MTLLVATRSSGKKAEARRLLGSAGIEAVFPDDIGVAVEPAEDLL
                     EMGETFQANARAKAEYFARRSGLPTVADDSGLEVLSLGGLPGVRSKRWAASVESGEKL
                     DHANNAELLRRLLGAPASRRRAQYRCVLAFVVTPGAVPEFFEGACTGTILESPRGNAG
                     FGYDPLFLSDDLEKTFGEAAAADKDAVSHRGRAFQQLIAHLAPR"
     gene            4054..4311
                     /locus_tag="VFB89_06615"
     CDS             4054..4311
                     /locus_tag="VFB89_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27010.1"
                     /translation="MRNLLALGAAAGLIAAGATLAAARTGNRHQTGAAPTYDIQCFGL
                     KTLGRDINAQARSGWTVKAMADHTSPEGQECVLVLFERATP"
     gene            4604..6901
                     /locus_tag="VFB89_06620"
     CDS             4604..6901
                     /locus_tag="VFB89_06620"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZM27011.1"
                     /translation="MPSPGANPRSASLIALGLWVGILTGFAELAKVGFDLLSSDFIYR
                     SRDASWMIPAFDAGLFGLLAVVIALMGLRVPVPWWFAAALLAGLGTALVLLLVERLHP
                     ASALVVAAGIGAQTARLVRPRVPAVQRVVRRTLPWLAGSLLLVAVGTVGWRVLAERRA
                     VLARSTAAGGAPNVLLLILDTVRAANLSLYHYPRPTTPELERFALGGTVFERAFAAAP
                     WTLPSHASIFTGQWETSLQVDDRHRLAPNYPTLAEAMHARGYATAAFVSNARWAGWES
                     GFTRGFERYDDHPISLWTALNAAAFGKQIYRRIRPLLAPKLTRLPVLWRLRLPQSDHH
                     RSAEDISRAFLAWVDRDQARPFFAFLNFMDAHLPYTAPDSFRFRFRSALPRLPSRYAW
                     SETPPVRLTPTDLRPKLDMYDGSIAYLDSQLGRLFDELERRELLDNTIVIVTADHGDE
                     FAEHGVVDHGSTLYRFALQVPLVIRFPGKVPAGRRVPEPVSLRNLAATVLDLTGPGAA
                     PLPGCSLARFWAPSAYVVPDTIIASNVRRRAGMLASIAFEDWRYIRNDETSTEELYHF
                     EGDSLERWNMVDSLAETSILDHYRAALAAVSVSGRPTLSDTTRLPAFPVGPAAAVPHW
                     SCGIASRGTQPPSRAHDRRKAPKTTSTHPSARAAQKPRRAKRRTGSSPPEARAEADSS
                     RRMSPVSDSVATQTRPSVEGNSDGTMDGRVCFGSGSRNAFVRQKCVGSAVIGVEGANP
                     DGGGSHPLRMHLGPASWATRQYRRD"
     gene            complement(7033..7521)
                     /locus_tag="VFB89_06625"
     CDS             complement(7033..7521)
                     /locus_tag="VFB89_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27012.1"
                     /translation="MNRPEQLGILLLGVVAAGMGLRTMRRREEVTIDEIGTVIATYRG
                     PAAFCRGVSAVLTGLGIVAAAFAGLLGLGAAMETQLRERPGALISFASLAGLAWSLSL
                     VFGSVEQRGSTLRLVAGALGRLLGVVLTTVSVAGLGLGVWEMVAPAQFDHLIALLSNG
                     PR"
     gene            complement(7771..8298)
                     /locus_tag="VFB89_06630"
     CDS             complement(7771..8298)
                     /locus_tag="VFB89_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZM27013.1"
                     /translation="MTDLTHVSTVTARTAGNVVASASLLRAKHSSNPFMHQRHTVLIV
                     DDADLVRRTANRMLTKGGYRVLEAADGDEALSVLSTRGVKVDLVLLDVVLPGKDGVAL
                     YADICARWPEMPIVFISAYSAEILVTYGQEDLSVPFLAKPFTYEQITSKVRAVIEQRI
                     HCREPRTSGPQPMKS"
     gene            8458..8649
                     /locus_tag="VFB89_06635"
     CDS             8458..8649
                     /locus_tag="VFB89_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HZM27014.1"
                     /translation="MRSIFTAMLADESGQGLVEYALIIALVAVGLIAILLVLRNSIGN
                     VFKNAASQLNNAPTAPFTP"
     gene            8797..9192
                     /locus_tag="VFB89_06640"
     CDS             8797..9192
                     /locus_tag="VFB89_06640"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZM27015.1"
                     /translation="MKKTESRSGFTPEGWHTVTPRIVAHDAERLVEFLKRVFGATGAY
                     RPDRPAEMKIGDSIVMVTDAGVRDPMPAFLYVYVDEADETYRKAISTGAKSLEEPSDQ
                     PYGDRRGMVRDQWGNTWQIATRRRRPDAP"
     gene            9379..9645
                     /locus_tag="VFB89_06645"
     CDS             9379..9645
                     /locus_tag="VFB89_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27016.1"
                     /translation="MPRRIIEVNGERWEVEVSGRVTQYVKDELGLVFTRGIGRDRERR
                     VTRYSPLGDKNSELALEQLSEADLRRLLAHSQPAWTAPETGYRP"
BASE COUNT         1704 a         3091 c         3171 g         1716 t
ORIGIN      
        1 cgaggattgt gctgccagct gtcgctcggc ccagacgcgc tgccgcgccg ctgccgagtc
       61 tacctcgggt ccgtgaaggc catgctctcc cagcattgcg gaaagccgat ggaccagggg
      121 gaagcccagc tccgggtgca gcgaccggta aaccaccgat gcggctcgac tggccaccag
      181 ccgctccatg actcgagtca gcgggcgcga gctgccgagc ccgtcgccat ggatcgcgag
      241 cccacggcgc ccgccggctt cgaactcgag ccgatgcgga gcgtagagaa tgccggcttc
      301 acggttccag aactcgagtc cccatcgatc gtgattgccg ccaaccatca ggatcggcac
      361 ctttcgcgca agcgcggcga gcgccgcggc cgtcctgaag gcatgccggg ggacgacggt
      421 gcgataggcg aaccagaatt cgaacaggtc gccgttgacg agcagcgcgt cgcccagtga
      481 ggggatgatg tcaaggaatt cgagcagcgc cgtttccacg gcagctggag cgtatccgag
      541 atgcgcgtcc gacaggacga tcagggtctc tcccggcacg ggcggaaact tgccgagggc
      601 ccgatgaccg ggcaacccgg gcaacatcga gtctgtgaga ttcaggtgcg ggtgaactac
      661 tccgagatcg atcagatggg cgttgtgtac catgcccgcc atctggtctg gctcgacatc
      721 gcgcggtgcg agtacctgcg tcaaaccggg ctgagctacc gggagctgga agcgtcaggg
      781 cttcggctcg tcgtcgggga ggttgcgatc cggtatcgtc agccggcccg ttatgacgat
      841 ctccttcggg tgcggtgctg ggttcgcgac gtcgcgtcgc gacgcgtcga gttcggctac
      901 gccgtggagc atgccgaaaa tcagcgtttg attgcgacgg ccagggtggc gctgcatccc
      961 ctcgacgaac agatggcatt gtgccggctg cctgacgacc tcagccgtcg cctcactccc
     1021 attccggatc cggtacgcct ttgaattccc ggatcgctcg aataccttcg ctctcgttcg
     1081 acgtcgcgag tccgcgtccg ttgtgggata ccttcatgga ccagccgttt catctcatcc
     1141 gtgttgtcgc cacggcgggc ttggttgctg ccgccggctg ccatactgcc ccgggtcctc
     1201 tgctgcctgc ggcggccgcg cccgtcagcc ccgagcaggt gacgggttgg gtgaatgcga
     1261 cggtgcccac ggaccaccgc ttgcttcgct tcaaatggct gtttcgagac gaaaggtcga
     1321 gcgccggcgg tcgcggcagt gcgcgcatcg cgccgcccga ttccctgcgc ttcgacgttg
     1381 cgggcccatt cggcagcaat ccagccgctg ccgcggtggt gggtgactct gccgtctggg
     1441 tccggcccga ggacgtgatc aagaagctgg tccccaacta ccccctcatg tgggctctac
     1501 tgggggtcgc gcgtcagccc gattcggggg ctgtgctgcg gggccttacc gagggccaga
     1561 ccaccatgtg gcagtacgca tcgggaactg acacggtgga gtacgtacgg accagcgaga
     1621 gcccgggcaa actgcttgca gaagtacgtc aggggagcaa ggtggtcggc cgagtggaga
     1681 cccagtttgg ggccgacggc tcgcctgcca gcgccaggct caccgttccc tccgttccgg
     1741 cccggctcga cctcaccttt ctttccatcg caaggaacag ttcgtttgcg ccgaccacct
     1801 ggcagcctcc tcctcctaag ccttagtctg ctggcactca gcggctgcta cgggttcgcc
     1861 ggcggcgggc tgccaccggg catcaggacg gtcgctgtgc taccattcga caatctgacg
     1921 cccgaaccaa cactgacgca ggaagtgcag aacgctgtcc ggaacgcggt gcagggccgc
     1981 ctgggactgc gccagtccgg cgagcagtcc gcggatgtca tcgtgcgcgg atccatccag
     2041 cgatacgagc cagatctgcc ggtggcgtat cagggagtgc agaacaacca ggtcgacgtt
     2101 acccgtcgcc tggtgcagat cacggtgaac gttgagatca ccaaccagcg aactggaaag
     2161 atcctgtatc agcggtcagg gcttaccctt cagggcgact acgcgaccgg gcaggaacca
     2221 agcgggcgcc agaaggccta cgatcaactg gtaacgaaca tcgtcgaagg agcgcaatcc
     2281 caatggtgag ctcctcccgc atcgaccgcc gaggcacgag cacgctcggc tgtttggtct
     2341 cgctgctcgt gttcgtggcc gcattgtact acggcattca catcggcgag gtctactggc
     2401 gctattacca gattctcgac gagatgaagt caaatgcccg ggtcgcgcct aatcttgccg
     2461 acgatgtcat ccagcgccgc ctccaagcca aggtgcaaga cgttttcggc gacgaggtgc
     2521 agatgaaatt ccgcatcaac cgcggcggcc gttcgcgtac aatcaccatc gacaccgagt
     2581 accgggacag cgtcgatctg ccgctgttca agcggggctt cgagctcaag ccccacgttg
     2641 aagagccgat ctgacgctgg aatacatgca tggcgcgtcc tgacggtcgt tcttccgagc
     2701 aactccggcc tctcgtgctc gagcgggccg cgaatccgta cgccgagggc tcctgtctga
     2761 ttcgtatggg tggaacgctg gtgcattgca cggcgacggt cgagcccggc gtgcccggct
     2821 ttaagaaagg gaccggcgag ggatgggtga ccgcggaata cgcgatgctg ccccgcgcga
     2881 cgctggagcg aaccccccgc gagcggaacg gtccgggcgg tcggacccag gagattcagc
     2941 gactgattgg acggtccctg cgcgcctgca tgtccacgat ggcgttcggc gaattcacca
     3001 ttcgggtgga ctgcgacgtg ctccaggccg acggcggcac ccggaccgcg agtattaccg
     3061 gcggctgcgt cgcgctcgcg gatgcctgcc agtggctggc tgacaagacc aagatcgctt
     3121 ccccgttcgg tcgactcgtc tccgctattt ccgtcggcat cgtcgagcgt gagattcgag
     3181 ttgatctggc ctacctcgaa gatcgcgacg cgggcgtgga cgcgaacgtc gtcatgctcg
     3241 agccggccca gttcgtcgag gttcagggaa ccggcgagca cgggaccttc agtcgcagcg
     3301 agctgggaac tctgctcgac ctggccgagc gttcatgcgc cgagctgttc gcggcgcagc
     3361 gccgcaccct cggctggtga ccctgctggt cgctacccga agctcaggca aaaaggcaga
     3421 agcgcggcga ctgctcggca gcgcaggcat cgaagcggta tttcccgacg acatcggtgt
     3481 tgccgtggag ccggccgagg atctcctcga gatgggagag acgttccagg cgaatgcccg
     3541 cgccaaggcg gaatactttg cccgccgctc gggcttgccg acggttgccg atgactccgg
     3601 cctggaagtc ctctcactgg gcgggttgcc gggtgtgcgc tccaagcgct gggctgcctc
     3661 ggtcgagagc ggcgagaagc tcgatcacgc caacaacgca gagctgctgc ggcggctgct
     3721 gggcgcgccg gcctcccgac ggcgagcgca gtaccgatgc gttctggcct tcgtcgtgac
     3781 tcccggcgct gttcccgagt ttttcgaggg cgcttgcacg gggacgattc tcgagagccc
     3841 cagggggaac gccgggttcg gttacgatcc cctctttctg tcggacgatt tggagaagac
     3901 cttcggcgag gccgccgcgg ccgacaaaga cgcggtgagc caccgagggc gtgctttcca
     3961 acagctgatc gctcatctcg ccccgcggtg acgtagactg cctctcctga agccgtgtcg
     4021 cgaacaaccg acctccaacc ggaaaccacc gccatgcgta atcttctcgc actgggggcg
     4081 gccgccggcc ttattgcggc gggcgcaacc ctcgcagctg cacgtaccgg aaatcggcac
     4141 cagaccggtg ccgcgccgac ctatgacatc caatgttttg gcctgaagac cttgggcagg
     4201 gacatcaatg ctcaggcacg ttctggctgg acggtcaagg cgatggcgga tcacacgagc
     4261 ccggaggggc aggagtgcgt cctcgtcctt tttgagcggg caacacccta ggcgtgcggc
     4321 cggaggcagg gcagcgcgtt gctgtgctgc taccagcaat cccgggaagc cgctatcgta
     4381 ctggtcgttc tcaatgggtg atcgcggggt gtggcgcagt ccggtagcgc gcctgctttg
     4441 ggagcagcta aggcaggcgc aagctgcgca gcctacacga aagatcagcg agttaattcg
     4501 aagagccggg cagcttgagc gcggggaggg cggcgcgcaa gaacgctcgc gtctaaaccg
     4561 aactcagccg gagcacatct tgaacgcatg gagcccaacc tcggtgcctt ctcccggcgc
     4621 gaacccgcgc tcggcgtccc tcatcgcgct tgggctctgg gtcggaatcc tgaccggctt
     4681 tgcggagctt gccaaggtcg gtttcgacct gctctcctcc gacttcatct accgcagccg
     4741 cgatgcttct tggatgattc cggccttcga cgcgggactt tttgggttgc tcgccgttgt
     4801 catcgccctc atggggctcc gggtgccggt accgtggtgg ttcgcggcag cgctgttggc
     4861 tgggctgggc accgcgctgg tgctgctcct cgtcgagcga ctccacccgg catcggccct
     4921 agtggtcgcg gccggcatcg gcgctcagac cgcgcggctg gtgcgtcctc gcgtcccggc
     4981 agtccagcgc gtcgtgcgcc gcacgctgcc gtggctcgcg ggatcccttc tgctggtcgc
     5041 cgtcggcaca gttggttggc gagtactcgc tgagcgtcgg gcggtgctgg cgcggtcgac
     5101 tgctgccggt ggggcgccaa acgtgctcct gctcatcctg gacacggtgc gagcggccaa
     5161 cctgagcctc tatcactacc cccgtccgac gacacccgag ctcgagcgct tcgccctggg
     5221 cggcacggta ttcgagcggg cgtttgcggc cgcgccgtgg accctgccat cgcacgcgtc
     5281 aatctttacc ggccaatggg agaccagcct ccaagtcgat gatcgccatc gactcgcccc
     5341 aaactacccc acgctagccg aagccatgca tgcgcggggc tatgcgactg cagcattcgt
     5401 ttccaacgca cgttgggccg gctgggagag cggcttcacg aggggattcg aacggtacga
     5461 cgatcacccg atcagtctct ggaccgcgct gaacgcggcg gcgttcggca aacagatcta
     5521 tcggcggatt cgcccgttgt tggcgcctaa gctgactcgc cttccggtcc tttggcgcct
     5581 gcggctgccg cagagcgacc accaccgctc tgccgaggat atctcgagag cttttctcgc
     5641 ctgggtcgat cgcgaccagg cgcggccctt ctttgcgttc ctgaatttca tggacgcgca
     5701 cttgccgtac acggcacctg actcgttccg gttccggttt cggtccgcgc tcccgcggtt
     5761 gccctcgcgc tatgcctggt cggaaacgcc gccggtccga ctcacaccca ccgatctccg
     5821 ccccaagctg gacatgtacg atgggtcgat cgcctatctt gactcgcagc ttggtcggct
     5881 gttcgacgag ctcgagcgtc gagagttgct ggacaacacg atcgtgatcg tcaccgcgga
     5941 tcacggcgac gagttcgctg agcacggagt cgtcgatcac ggaagcacgt tgtaccgctt
     6001 tgcgctccaa gtgccgctcg tgattcgctt cccagggaag gttcccgcgg gccgccgagt
     6061 gccggagcct gtttctcttc gaaatctcgc cgccacggtc ctggacctta ccggccctgg
     6121 cgcggcgcca ttgccgggct gctcgctggc ccgcttctgg gcgccatcgg cttatgttgt
     6181 gcccgacacg atcatcgcga gcaacgttcg ccggcgcgcc ggcatgctgg cctccatcgc
     6241 tttcgaggac tggcggtaca tccgcaacga cgagaccagc accgaggagt tgtaccattt
     6301 cgagggggat tcactggagc gttggaacat ggtggactcc ctggcggaaa catcgatcct
     6361 ggatcactac cgtgctgccc tggcggcagt gagcgtgagt ggcagaccga ctctgagcga
     6421 cactacgcgg ctgccggctt tcccggtcgg gccggcggcg gccgtcccgc attggtcctg
     6481 cggtatcgcc tcgcgcggaa cccagccgcc gagccgcgct catgataggc gcaaggcgcc
     6541 gaagaccaca tcaacacacc ccagcgccag agcagcgcaa aagccccggc gcgccaaacg
     6601 gcggactggc agctcgccgc ccgaagccag ggccgaagcc gactcgtcaa gaaggatgag
     6661 tcccgtaagc gacagcgtag ctacccagac cagaccctcg gtcgaaggga acagcgatgg
     6721 cacgatggac ggacgcgtct gctttgggag cggctcacgg aacgccttcg ttcgtcagaa
     6781 gtgcgtgggg tcagcggtga taggcgttga gggtgcgaat cctgatggcg gcggttcgca
     6841 ccctttgcgt atgcatcttg gcccagcatc ttgggccacg cgccagtacc gaagggacta
     6901 gggcgcaggc gcgtgtccgc tgtgccccgg actctggccc gggcgccctc aagtacagga
     6961 ctggcgaatg ggcattcacg acagcagaag aagtcgcaca ggctctagcg gcccacctgg
     7021 ccaatacatc gattagcgcg ggccattaga gagcaacgca atcaagtggt cgaattgggc
     7081 aggcgcgacc atttcccaca ccccaagccc gaggcccgcg actgagacgg tagtgaggac
     7141 cacgccaagc aggcgaccga gagcgccggc cacgagtcgc aaggtcgagc cgcgctgctc
     7201 gaccgagccg aacacgaggg aaaggctcca ggccagcccc gcgagcgagg cgaaggaaat
     7261 cagcgccccg ggccgctcgc ggagctgggt ttccatggca gccccgaggc ccagcagccc
     7321 ggcgaaggca gcggccacga tgccgaggcc ggtgagcaca gcactgaccc cccggcagaa
     7381 tgcagccggc ccgcgatagg tggcgatgac cgtgccgatc tcgtcgatcg tcacctcttc
     7441 gcggcggcgc atggtccgga gacccatacc cgctgccacg actccgagca gcaggatgcc
     7501 aagctgttca ggtcgattca tggtacagcc gtctttctgt ccgtacgaga ttcccacctt
     7561 cgccgcgagc ccactcgctg gcgccgcgag cggggggtgg ggaccgggat cccatgcagc
     7621 gccgattgca actaactagc agtctgctga ctgcccgaaa gcgtggcacc ccgccatact
     7681 gtgaggcgtg accgtgccag caggaatctc gtaagtgctt tcattgtgcg tgcagaacga
     7741 ctatggcgcg agcccggctg accctggccg ctagctcttc atgggctgtg gtccagacgt
     7801 gcgaggctcc cggcagtgaa tccgttgctc tatgacggcg cgcactttcg acgtgatttg
     7861 ctcgtaggtg aaaggcttgg ccaagaaggg cactgagaga tcttcctggc cgtaggtgac
     7921 cagaatctct gctgagtagg ccgatatgaa gacgatgggc atttccggcc agcgggcgca
     7981 gatgtccgca tagagggcga ccccatcctt gccggggagg accacatcga gcaagacgag
     8041 atcgaccttc acgccccgcg tgctcaagac gctgagtgct tcatcgccgt cagccgcttc
     8101 cagcacccgg tagccacctt tggtcagcat gcggttggca gtccggcgaa ccagatccgc
     8161 gtcatcaact atgagaacgg tgtggcgctg atgcatgaac ggattgctcg agtgcttggc
     8221 ccgtagcaat gatgccgaag caaccacgtt ccccgccgtg cgagccgtca ctgtgctgac
     8281 gtgggtcagg tcggtcaatg gaggagggcg tccctcaggc ctaattcgtt gacgacgatc
     8341 gggcaatcgc tgatcgcggc tcggaagacg tacggcgaga tccggcacga ggacttatcg
     8401 gattgaagcc tcaacggtag tttctcgaaa gcgccaccac gcaccacgga ggaacatatg
     8461 aggtcgatat tcaccgcaat gctcgcggac gagtccgggc aggggctggt ggaatacgcc
     8521 ctgattatcg cgctggtcgc ggtcggctta atcgcgatcc tgctcgtgct ccgcaactcc
     8581 attggcaacg tcttcaagaa cgcggcgtct cagttgaaca acgcaccaac ggcccccttt
     8641 acaccctgag tgggcgagag tagagggccg ccgatgcgac ctcggcttac gcctcggtcg
     8701 ttgtggaagc gttgaattgt gaatgcgcgg gtttgtgccc gtagctgaag aggggtcgtt
     8761 aggcggcagc gtcgttatga gcggaaaggc caagtgatga agaaaacgga gtcccgctcg
     8821 ggcttcacgc ccgaggggtg gcataccgtg actccacgaa tcgtggcaca cgacgcagaa
     8881 cggttagtcg agtttctcaa gcgtgtgttc ggtgcgacag gcgcctatcg tccggatcga
     8941 ccggccgaga tgaagatcgg cgactcgatc gtcatggtta cggacgctgg agtccgagat
     9001 ccgatgccgg cctttctcta tgtgtatgtg gacgaggcgg atgagacata tcggaaggca
     9061 attagcacag gagctaagtc actggaagag ccgtcggacc aaccctacgg ggaccgtcgc
     9121 ggtatggtaa gggaccagtg gggcaacacg tggcaaatcg cgactcgccg cagacgtccc
     9181 gatgccccct aacccacgct tgcagctgac gggcgagctc gtttcagagg cacgtagaat
     9241 tcggaatcgc cgggctgatc gccccgcagc tgaagcgtag agcgtcagac cgcaccaaga
     9301 gatgccgtag ttctccaaaa cgctcgcgag gctgcgcccc gaacgcttgg gagctctgtg
     9361 aggaggtggc gctctgacat gccgcgacgc atcatcgagg tgaacggcga gcggtgggag
     9421 gtggaggtga gtggccgggt gacccagtat gtgaaggacg agctcgggct cgtcttcacg
     9481 cggggcatcg ggcgcgaccg cgagcgtcgg gtgactcgct actcgcccct cggcgacaag
     9541 aactcggagc tcgcgctcga gcaacttagc gaggccgacc tccgccggtt actcgcgcac
     9601 tcgcagccgg cctggaccgc gcccgagacg gggtatcgtc catgatcgag ggggcaatcc
     9661 ccgggtggcc cccggcgcgc ag
//
LOCUS       DASRJQ010000297         2812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1447609, whole genome shotgun sequence.
ACCESSION   DASRJQ010000297 DASRJQ010000000
VERSION     DASRJQ010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447609"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..445)
                     /locus_tag="VFB89_06650"
     CDS             complement(47..445)
                     /locus_tag="VFB89_06650"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZM27017.1"
                     /translation="MKIGQVAAQAGVSIDTVRFYERRGVLPAPERTPSGYRIYEASTV
                     ERIRFARALQHLGFTLDEAIDALRSHDTGAATCEAELWRMQAVIDRIDTKIAELQGTR
                     RHVSETIDACQQGHCRFGTRTMPTPAAQRH"
     gene            complement(582..1445)
                     /locus_tag="VFB89_06655"
     CDS             complement(582..1445)
                     /locus_tag="VFB89_06655"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZM27018.1"
                     /translation="MSEFPTLRAAIAGTIDVGGDLTDNRLGFGAMRITGNGIWGPPAD
                     RDEAKAVLRRAVELGINFIDTADSYGPHISEELIKEALHPYPDDLVIATKGGLERTGP
                     GQWPANGRPEHLIEACEGSLRRLELEQIPLYQFHRPDPAVPLEDSIGAIVTLKGQGKI
                     RHIGLSNVTEEQLRRAQRLTPIVSIQNRYNVDDRRSESLVDLCEQEQMVFLPWAPIQN
                     LDSNRTVQQFAQRYDATPRQIVLAWLLARSPSILPIPGTGSVSHLEDNVAAAAIKLTP
                     AEVASVKDSVG"
     gene            complement(1768..2178)
                     /locus_tag="VFB89_06660"
     CDS             complement(1768..2178)
                     /locus_tag="VFB89_06660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HZM27019.1"
                     /translation="MDAAEALAFARRSPRAVLATMKRDGTPQLSPVLVGVDSQDRLVV
                     STRETAMKVLNVRRDPRAWLCVLNDRFFGDWAQLSGDVEVVRLPEAMEPLVEYYRSIS
                     GEHPDWDDYRTAMIKQGRCLIRITPTKAGPTVFG"
     gene            2412..2576
                     /locus_tag="VFB89_06665"
     CDS             2412..2576
                     /locus_tag="VFB89_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27020.1"
                     /translation="MKWVKGFIVFLVVGFALFYLIAYPEAAADAVRAVAGGLAIVFRS
                     ILIFFQSLAG"
     gene            complement(2631..>2812)
                     /locus_tag="VFB89_06670"
     CDS             complement(2631..>2812)
                     /locus_tag="VFB89_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27021.1"
                     /translation="RLAEGRQWQACSIRVDDKDEALDLYVNKLAPEAGQIAQCFCPVG
                     CRLASWAGLLQWQGE"
BASE COUNT          467 a          863 c          917 g          565 t
ORIGIN      
        1 accgctgtgg atgggctctt cggcgacctg cacaaagcga gcatggtcag tgtcgctggg
       61 ctgcgggtgt cggcatcgtc cgggtgccga accggcaatg tccttgctgg caggcatcta
      121 tcgtttcgct gacatggcgg cgagtcccct gcagttcagc gatcttcgtg tcgatgcggt
      181 cgatgacggc ttgcattcgc cacagttctg cttcgcacgt tgccgctccg gtgtcgtggc
      241 tgcgcagcgc atcgatggct tcgtcgaggg tgaacccgag atgctgcaat gctcgggcga
      301 accggatgcg ttcgaccgtt gacgcctcgt agattcggta gcccgaaggc gtccgctcgg
      361 gcgccggcaa cacgccccgc cgttcgtaaa aacgaacggt gtcgatgctg accccggcct
      421 gcgcggcgac ttgtccgatc ttcatgccgg tttctccttg actctggact taggtcaaga
      481 gtttagggtt gggtcatcgc ggacgcgatg ggcgttcgaa ccgtccttac cagatcgcgg
      541 tcgagaacgg tcaggtgctg tgggacatca ccactatcga ctcaaccgac gctgtccttc
      601 acagaagcaa cctcggccgg ggtcagcttg atcgccgcgg cggcgacatt gtcttccagg
      661 tgcgacaccg atccagtccc gggtatcggc aggatcgagg gcgatcgggc cagcagccag
      721 gccagcacta tctgtcgcgg tgtcgcgtcg tagcgctgcg cgaactgttg aaccgtccgg
      781 ttgctatcaa gattttggat cggcgcccag ggcaggaaga ccatctgctc ctgctcgcag
      841 aggtcgacca gcgactccga ccgccggtcg tcgacgttgt agcggttctg gatcgacacg
      901 atcggggtga gtcgctgcgc ccgtcgtagc tgctcttcgg tgacgttgga cagcccgatg
      961 tgtcgaatct tgccctgccc cttgagggtc acgatggctc cgatcgagtc ctccagtggc
     1021 actgccggat cgggccggtg gaactgatag agcgggatct gctccagctc cagccggcgc
     1081 aggcttccct cacaggcctc gatgaggtgc tcaggtcgac cgttggccgg ccactggccg
     1141 ggaccagtgc gctcgagccc gcccttggtt gcgatcacca gatcgtctgg gtacgggtgg
     1201 agtgcctcct tgatgagctc ttcgctgatg tgaggcccgt aggagtcggc ggtgtcgatg
     1261 aagttgatgc cgagctcgac cgcgcggcgc aacaccgctt tcgcctcgtc gcgatcggcg
     1321 ggcggacccc agatgccatt accggtgata cgcatggcgc cgaagccgag gcggttgtcc
     1381 gttaaatcgc cgcccacatc gatcgttccg gcgatcgcgg cgcggagggt cgggaactct
     1441 gacatggatc gtgactcctc tggtctgcgg ctttcgtggc cgctgccact gtatgtctgt
     1501 cgagaatcgc accggtcgcg gccgtttgaa agggcaacgc cgtccgggat cgccgccgcc
     1561 gactcggtct gctgtcgtca ggggccgggt gcgctactcg tcctgcgcgt ctcccgggtg
     1621 agcggatggt caggcaacgc ggctgcccgg cgcgcgtaat cccgcggggc caacggcgtg
     1681 aagcctgccg gttggatccg ccagcctatt gtcgggtccg ggtgggccga tcctggtaga
     1741 cgatcgtcgc cagggggcca aaagctatca accgaagacg gtcggaccgg ctttcgtcgg
     1801 cgtgatccgg atcaggcagc ggccctgttt gatcatggcg gtccggtagt cgtcccagtc
     1861 cggatgctcc ccggagatgg atcggtagta ctccaccagc ggttccatcg cctcagggag
     1921 cctgacgacc tcaacgtcgc cgcttagctg tgcccagtcg ccgaaaaacc tgtcattgag
     1981 cacacacaac caggctcggg gatcgcggcg gacgttcaag accttcattg cggtttcgcg
     2041 ggtactgacg accagacgat cctgcgagtc gacgccgacg agtactggtg ataactgggg
     2101 agtgccgtcg cgtttcatcg tggcaagaac ggcacgtggg ctcctgcggg cgaacgcgag
     2161 cgcttctgct gcatccatat cgcaagcaag tcaatcagaa gaccgtgggc cgcaggcgca
     2221 agaggtcagg tcacgcgaaa cggtgacgag agtgcggcag gcacctaccg cgggagaaac
     2281 aggaggcccc gagggcttcg ccctgagctc tcccctcctc tgtgccagaa ggagagcatc
     2341 gatgcatcgg cctgtacaag cagcgggctg ttcgaatagc ctttgaagac cggaaggatg
     2401 accagtccga catgaaatgg gtcaaggggt tcattgtctt cttggtcgtg ggcttcgcac
     2461 tgttctattt gattgcctac cctgaggctg ccgcggacgc cgtccgcgcc gtagctggtg
     2521 gccttgcaat agtctttcgc tcgatcctca tcttcttcca gtctctggct ggttagcgtg
     2581 ggtgtgctga actggctcga tccgcacgcc acggttggtg agggacgcag tcactcaccc
     2641 tgccactgca gcagtcctgc ccaggacgcc aaccggcagc ccaccgggca gaagcactgg
     2701 gcaatctggc ccgcctccgg tgcgagctta ttgacgtaga ggtcgagagc ctcgtcctta
     2761 tcgtcgacac ggatggagca tgcctgccat tggcgacctt cagctaaccg ct
//
LOCUS       DASRJQ010000298         4292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3997418, whole genome shotgun sequence.
ACCESSION   DASRJQ010000298 DASRJQ010000000
VERSION     DASRJQ010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3997418"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..1057)
                     /locus_tag="VFB89_06675"
     CDS             complement(29..1057)
                     /locus_tag="VFB89_06675"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HZM27022.1"
                     /translation="MSETDLFAQQEANRRRSTWLVVGFVLFFAWVGFGGDLAFGLLTR
                     NAPSGAYHHVVPIIGIVATLLAGGICWYSWRYGAERVLWATGAWEVVEPATLEQKQLV
                     NVVEEMAIAAGLPKPRIWIVPDDDPNAFATGHDPNTASIAVTEGLLKVLNREELQAVI
                     SHEMSHVRNLDTRLMTLLAAMVGSIALMSDGMGRMMRLGSFGGTGGGEKGEKGKGSPL
                     GLVVLVFWLLTLIVAPIVSRVLAMAVSRKREYLADATGAQLTRNPGALASALEKLAAA
                     SAPTRAITLGAAHLCIVDPTGNRFANAEGFLGDVFASHPPIAQRIIRLKGMAYQDAKR
                     SGAQIQTA"
     gene            complement(1054..1629)
                     /locus_tag="VFB89_06680"
     CDS             complement(1054..1629)
                     /locus_tag="VFB89_06680"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HZM27023.1"
                     /translation="MGLILFLVVIAVIVLWIIGAYNGLVALKNRVANAWKQIDVQLKR
                     RHDLIPNLVNTVKGSMDFERSTLEAVISARNKAVSATGVQATAKAEGELTQALGRLFA
                     LTENYPDLKSNQNVLQLQEELTSTENKIGFARQLYNDTTTEYNTRQMQFPTNLVAGLA
                     KASPADLWEIQDAAERAVPTVDLSLPKPPAA"
     gene            complement(1710..2366)
                     /locus_tag="VFB89_06685"
     CDS             complement(1710..2366)
                     /locus_tag="VFB89_06685"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZM27024.1"
                     /translation="MTVMLSPAPVASPAPDPLATLREYRSLAPWGLRDLTAIAAGILD
                     VSGVVPVSPVARSRPTERTIRFYVARGLVSPPEGRGTAATYGYRHLLQVLAVKLRQME
                     GATLEAIVREMGGVTGDTVERRVASTLGPRLPGPSSLELERGPRSGRGRVGRALDGWI
                     APPPTGSSSEPTSCRKIPVGPGVELIVDDRHPALRRQDATSTLAAGVRSLLEGLLHGT
                     "
     gene            complement(2363..2758)
                     /locus_tag="VFB89_06690"
     CDS             complement(2363..2758)
                     /locus_tag="VFB89_06690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZM27025.1"
                     /translation="MKPRVLIIDDETSLRHTLERALRSFGYEVDSAPDPLSAYQLLGD
                     ATYDVVLLDLRLKQAMGDALYLALVRQWPYLRGRVMLMTGDPWFAQDSWPPELVACPI
                     LVKPFTLETLSRLVARIAKPDKRTKNGSA"
     gene            complement(2755..3546)
                     /locus_tag="VFB89_06695"
     CDS             complement(2755..3546)
                     /locus_tag="VFB89_06695"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZM27026.1"
                     /translation="MPILAAVPGERLFRLHAAEPEGYEWVPAASWVQAVTVIRTRPVE
                     LAVVDPTLGGEPRSMEIERLRQHFPSLPLVIYTTLAPETAGVLLDLGRAGIRRVLFHR
                     FDDAPGALRRALHAELEQSASQRLMQQLTAILPPLPEPLRRALEATLHAPGDGPTVSV
                     LAARAQLKRRTCERWFARLGLPSPKMVMLLARVLYAHRLLLDPGYTVEDVAVKLGYTR
                     AKTLQMHFREVFGLTAGEVRLSLSSDEALRLITERHLRQPDQVAS"
     gene            complement(3829..>4292)
                     /locus_tag="VFB89_06700"
     CDS             complement(3829..>4292)
                     /locus_tag="VFB89_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27027.1"
                     /translation="LSDLGYYDVARIAFGIVVGSNTSFIVKTNARLELMEIESASGNR
                     VAFERHRGELRDVASRMTPSMAIDYRFKAGTGLARFGQFARAREAWAEGMRLAEAHHL
                     NEWYFRLERLSQQLDGCAAPQSSGAEPQTPPAAIADLAAGLTAYAEEAMPA"
BASE COUNT          801 a         1395 c         1347 g          749 t
ORIGIN      
        1 agggtagata gacaaaccgc gtaaacggtc acgcagtctg tatctgtgct ccgctcctct
       61 tcgcatcctg atacgccatt cccttgagcc ggatgatccg ctgtgcgatc ggtgggtgag
      121 aagcaaagac gtcacccaga aagccttccg cattcgcaaa ccgattgccg gtcgggtcca
      181 cgatacaaag atgcgcggcg cccagcgtga tggcccgcgt cggggcggag gcagctgcaa
      241 gcttttccag cgcggacgcg agcgcgccgg gatttcgggt caactgtgcg ccggtggcat
      301 cggccagata ctcgcgcttt cgactcaccg ccatcgcgag cacccgcgag acgatcggag
      361 caacgatcag cgtgagcaac cagaagacca ggactacgag gccgagcggg ctccccttcc
      421 ccttttcccc tttctcccct ccgcccgtcc ctccgaagct tcccagccgc atcatccggc
      481 ccatgccgtc cgacatgagg gcaattgacc ctaccatcgc cgcgaggagc gtcattaggc
      541 gggtgtcgag gtttcggacg tgggacatct cgtgcgagat gaccgcctga agctcctccc
      601 ggttcagcac ctttaacagc ccttcggtga cggcgatgct cgcggtgttg ggatcgtgac
      661 cggtggcgaa cgcattggga tcgtcatctg gaacgatcca gattcgcggc ttgggcaggc
      721 cggctgcgat tgccatctcc tcgacgacat tgaccagctg cttctgctcc agcgtcgccg
      781 gctcgacgac ctcccaggcg ccggtcgccc acaacacccg ctctgctccg tagcgccacg
      841 agtaccagca gattccgccg gccagcaggg tggcgacgat gcctatgatg gggacgacat
      901 gatggtacgc tccggaagga gcattccgag tcagcagacc gaacgcgaga tcgcctccaa
      961 agccgaccca ggcaaagaag agaacgaacc ccaccaccag ccatgtcgaa cgccgccgat
     1021 ttgcttcctg ctgcgcaaaa aggtcggttt cgctcacgcg gccggcggtt tcggtaggga
     1081 gaggtcgacc gtaggtaccg ccctctcggc tgcgtcctgg atctcccaca gatcagcggg
     1141 tgaggccttg gccagtccag cgacgaggtt ggttggaaac tgcatctgcc tggtgttgta
     1201 ctcggtggtg gtatcgttgt agagctggcg cgcgaagcca atcttgtttt ccgtgctcgt
     1261 cagctcctcc tgcagctgaa gcacgttctg attggacttg aggtcgggat agttctccgt
     1321 caatgcgaac agccggccaa gcgcctgcgt cagctcaccc tccgctttgg ccgtcgcctg
     1381 cactccggtc gccgacaccg ccttgttgcg ggccgagatt acggcttcga gggtcgagcg
     1441 ctcgaaatcc atggagcctt ttacggtgtt caccaggttc ggaatcagat cgtggcgccg
     1501 cttgagctgc acatcgatct gtttccacgc attcgcgacc cggttcttca gggcgaccag
     1561 cccgttgtag gctcctatta tccacaacac gattacggcg atgacgacca ggaaaaggat
     1621 cagtcccatg gaccgtcctc cgatgttgag tgggtatcag ccctgaagca ggtgcgccgt
     1681 cgggcctgga cgggatggag accagtcgct caggttccgt gcagcagccc ctccagaagc
     1741 gaccggacac ccgcggccag cgtggaggtg gcgtcctgcc ggcgcagagc gggatgccgg
     1801 tcgtccacga tcagctctac gccggggccg accgggatct tccggcagga ggtgggctcc
     1861 gagctgctgc ccgttggggg tggggcaatc catccgtcga gggcccgccc cacccggccg
     1921 cggccggacc gagggccacg ctccaactcg agcgaggatg gacccggcaa ccgagggccg
     1981 agcgtcgatg cgacccgccg ctcgacggta tcgccggtca cgccgcccat ttcacggacg
     2041 atggcctcca gcgtggcgcc ttccatctgc cgcagcttga ccgccagcac ctgaagcagg
     2101 tggcggtagc cgtaggtcgc agcggttccc cggccttcgg gtggactcac caggccccgg
     2161 gcgacgtaga accggatagt ccgttcagta ggacgggatc tggcgaccgg gctcaccggc
     2221 accacgccgg acacatccag aatgccggcg gcgatggcgg tgaggtcgcg cagaccccag
     2281 ggcgcgagcg agcggtattc ccgcaacgtc gcgagcggat ccggggccgg actggcgacc
     2341 ggtgccggag agagcatgac agtcatgcgc tgccgttctt tgtccgtttg tccggcttcg
     2401 cgatacgggc gaccagtctc gacagcgtct ccagcgtaaa cggcttgacc agaatcggac
     2461 acgcgactaa ctccggtggc caggagtcct gtgcaaacca tggatcaccg gtcatcaaca
     2521 tgacgcggcc tcggagatac ggccattggc ggacgagcgc aaggtagagg gcgtcaccca
     2581 tcgcctgctt gagccgcaga tcgagcagca cgacatcgta cgtcgcatct ccgagcagct
     2641 ggtacgccga cagaggatcg ggcgcactat cgacttcgta accgaacgac cgaagtgccc
     2701 gctccagcgt atggcggagg gacgtctcat catcgatgat gagaacgcgg ggtttcatga
     2761 ggctacctga tccggctgac ggagatgccg ctcagtgatg agtcgcaagg cttcgtccga
     2821 tgacaaggac aggcgaacct ctcccgccgt taggccgaac acctcgcgaa aatgcatctg
     2881 cagagtcttg gcccgggtgt aaccgagttt cacggcaacg tcctccaccg tgtagccggg
     2941 atcgagcaac aagcgatgag catatagcac tctggccagc aacatcacca tcttgggcga
     3001 cggcagcccc agccgggcga accagcgctc gcaggtgcgc cgcttgagct gggcccgtgc
     3061 agcgagtacc gacacggtcg gaccatctcc gggcgcgtgc agtgtggcct cgagcgctcg
     3121 gcgcaggggt tccggcaggg ggggaaggat ggcggtcagc tgttgcatga gccgctgcga
     3181 agcgctctgc tccagctcgg catggagcgc tcgcctcagc gcgcccggcg cgtcgtcgaa
     3241 gcggtggaag agcacccggc gaataccggc ccggcccaga tccaggagaa ccccggcggt
     3301 ctcgggagcg agggtggtgt agatgaccaa aggaagcgag ggaaagtgct gccggagtcg
     3361 ctcgatttcc atcgagcggg gttcgccgcc gagtgtcggg tcgacgacag cgagctctac
     3421 tgggcgagtc cgaatgaccg tgacggcctg tacccagctc gccgccggaa cccattcgta
     3481 cccttcaggt tcggcggcat gaaggcgaaa caaccgttcg cccgggacgg ccgcgagaat
     3541 cggcacctgt cgcaaaagcg cccctcaatg tcgcaaaaag tacctaaccc aaaaccgcca
     3601 agcgcacaca acttggcgcc agccccctta gatgagcttg atctcggagg tttgcatcat
     3661 gtctcgctcg cgtacgctag cgctcgcagt gtggcttgcc gccggcctgc tggctgcttg
     3721 ttcagatact accggaccgc gccccagcct tcgctgcgaa acgcaaggat cgaatagcac
     3781 tcgcaccacg gctacagcct gcatgaaata gatcgctgcc cgatcagctc aagcaggcat
     3841 tgcctcctcg gcgtaagccg tcaggcctgc ggccagatcg gcgatcgccg ccggaggcgt
     3901 ttgaggttcc gcccccgaac tctgcggtgc ggcgcagcca tccagttgtt ggctcaagcg
     3961 ttctagacgg aagtaccact cgttgaggtg atgggcttcg gccagtcgca tgccttccgc
     4021 ccatgcctcg cgggcccgcg cgaattgacc gaatcgcgcc agcccggttc ctgccttgaa
     4081 tcggtagtcg atggccatgc tcggcgtcat tcgcgaagcc acgtcccgga gctcgccgcg
     4141 atgccgctcg aacgccaccc ggttaccgga cgccgattcg atctccatca actcgagccg
     4201 ggcattcgtc ttgacgatga agctcgtgtt cgagcccact acgatgccaa atgcgatccg
     4261 ggcgacatca tagtaaccca ggtctgacaa ct
//
LOCUS       DASRJQ010000299         4091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2129082, whole genome shotgun sequence.
ACCESSION   DASRJQ010000299 DASRJQ010000000
VERSION     DASRJQ010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2129082"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1785
                     /locus_tag="VFB89_06705"
     CDS             <1..1785
                     /locus_tag="VFB89_06705"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HZM27028.1"
                     /translation="DSAEVAARAHDSVMSNQLDVPMLPPILAASGRSDEAVTIARAWA
                     RKQVGQGNVHGAALATNALAELLLRNGDARAALREAAAAESLAQRINLTDQLIRACRL
                     QGQSRLGMNDRAGGLRLLNHAAVMAAAHPSAEAVLLSQLALGDALAQASLPDQALSAY
                     DRAGRVVEDMTVRLDRDFERARFRHGQLAAFDGAVGILLRQPATPRRLAQLAAWSQRR
                     KAASLALAAGGHAGSVGARTSGLEDLRRRLAPGTALVDYQTLDSTSAALVVTARGARV
                     IALPIRPDSLRLLVDRLRQPLARSYAGRIDLARAPFDLAVAHALYRAVMVPLTPLLGG
                     IERLYIVPDGPLHLVSFDALVVEPPVDPANPAAYAAARYAVDRFQIEILPTAQFLPSR
                     DSGPVTADPTRVLAINRDAPGGESEVAAIRAAWPAGQVTVLEGADASETAVRKATGKY
                     TILHFATHAEADDREPLASHLRLGADSVADGFFHLEEIAQDRRPVRLAVLSACETLSG
                     RLYQGEGLMGLARAFLAGGAQAVVATQWPIGPASADLMGEFHRALAGGHEPGAALRSA
                     KLVLRRQPATAHPFYWAGVILIRGARAD"
     gene            complement(1797..2447)
                     /locus_tag="VFB89_06710"
     CDS             complement(1797..2447)
                     /locus_tag="VFB89_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27029.1"
                     /translation="MRALPLLLVLLLGGVPAAAVSTYKGLQPGKSTRADVERTLGQPV
                     RAVSATLFEYSLADGPGSILVEFRADAVVDRIERRFLKPVSRSALLRSLGLPETPEEK
                     GTNREGKLVEYFGDVNTLALTFASGEARSGVMSVGYYSMELFERGLGKARNPVVQFDP
                     AACRELYYWSQGERDAAKRAKNVGRHQEILEIQILAQRGECVKAQSLAAKYKESYR"
     gene            complement(2448..>4091)
                     /locus_tag="VFB89_06715"
     CDS             complement(2448..>4091)
                     /locus_tag="VFB89_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27030.1"
                     /translation="YQANQPPELGWWQKLKNGLTGGGAINPTNSPPPVTTAMQCQPVI
                     EKNRALARQQAIREKHLNDYQNVDQNSPNAAGSRTASLQAIKNSEDQMAADLTALRTA
                     VQDCHKELQDGLKKVGTKRTELEQLDPGNAIRDRQEELTKLEADKSLETERQRLVDEQ
                     KLLGDLVTRKEADVKNSRVMKEGADAMLDEVNKIIEIEANRDAVTNLKPAYDAAVTDK
                     NLMLQAANTNLAATRTFLLDWTAVRAANDPYQTHKKTFLEQKKTWEDRVKAWQTTKDP
                     ATAKALGDERTRLAADKVVLLREKAALVAVLRARNLNYKNLCERKKQLADALKSAKAD
                     LDTYKCFTDAATAITEIDTRTSELGAFIEEPRVLVGGPSQLESWDPSVASVELREPTL
                     LEALACKVEDFQLEKDLDALAEEIVLAEFDPTTDEPTDSVPGDLSKNTGAVKVPRYYV
                     FLLTNASNGLYVGDEDGIKQSTRCGFVGGGIGCKPTDLISYRKLLGPFASQDEAQAAL
                     CKSITESRIFPIGIGMKGRWQGGNTWYGLWNASVSGCPR"
BASE COUNT          595 a         1372 c         1321 g          803 t
ORIGIN      
        1 gacagcgcgg aggtggctgc ccgagcgcac gacagcgtca tgtccaacca gctggatgtg
       61 cccatgcttc cgccgatcct cgcggcaagc gggcgaagcg acgaagcagt gaccatcgct
      121 cgcgcgtggg cgcgaaaaca agttgggcaa gggaatgtgc atggcgcggc gctcgcgacc
      181 aacgctttgg cggagctgct actgcgcaac ggggatgccc gagctgccct ccgcgaggcg
      241 gccgccgccg aaagcctggc gcagcggatc aatctcaccg accaactgat ccgcgcatgc
      301 agacttcagg gccagagccg gctcggcatg aacgatcgag ccggtgggct ccggctcctc
      361 aatcacgccg cggtgatggc ggcggcgcat cccagcgccg aagcggtgct cctgtcccag
      421 ctcgcgctgg gagatgcgct ggcccaggcc tcgctgccgg atcaggctct cagtgcctat
      481 gatcgcgcgg ggcgcgtggt cgaggacatg accgtgcgtc tcgaccgcga cttcgagcgg
      541 gcccggttcc ggcacggtca gctcgcggcc ttcgatggag ccgttggcat cctgctccgg
      601 cagcccgcca cgccaaggcg gctcgcccag ctggccgcgt ggtcgcaacg ccgcaaggct
      661 gcgtccctgg cgctggccgc gggaggacat gccggttccg tcggtgccag gacatcggga
      721 ctggaggatt tgcgacggcg cctcgcaccg ggcaccgctc tggtggatta ccagaccctg
      781 gattcgacct cggcggcgct cgtggtcact gcccggggcg cccgggtgat cgcgctgcca
      841 atccggcccg actcgctccg cttgctggtg gatcgcctgc gtcagccact ggcgcggtca
      901 tacgccggca gaatcgacct cgcccgcgcc ccgttcgacc tcgcagtcgc gcacgcactg
      961 taccgcgcgg tgatggtgcc gctgactcca ctgctgggcg gaatcgaacg gctgtatatc
     1021 gtaccagacg gcccgttgca tcttgtctcg ttcgacgccc tggtcgtcga gccgccggtc
     1081 gatccggcga acccggcggc gtacgccgcc gctcgctacg cggtggatcg cttccagatc
     1141 gagatccttc ccacggctca gttccttccg tcgagggaca gtggtcctgt gactgccgat
     1201 ccgacccggg tgctcgcgat caaccgggac gctcccggtg gcgagtccga ggtggcggcg
     1261 atccgggcag cctggcccgc gggacaggtc accgtgcttg aaggagccga cgcgagcgag
     1321 accgcggtcc gcaaagccac cggcaaatac accatcctgc acttcgcgac ccatgccgag
     1381 gcggatgatc gcgagccgct ggcctctcac ctgcgactcg gggccgacag cgtggctgat
     1441 ggcttttttc atctcgagga aatcgcccag gaccggcgac cggtacggct cgcggtgctc
     1501 agcgcctgtg agaccctgtc ggggcgtctc taccagggcg agggcctcat ggggctggcg
     1561 cgggcgttcc tggcaggagg tgcgcaggcc gtcgtcgcga cccagtggcc gatcggtcct
     1621 gcctcagcgg atttgatggg cgagtttcac cgcgcgctgg ccggcgggca cgagcccgga
     1681 gccgctttgc gcagcgcaaa actggtgttg cgccggcagc ccgccaccgc gcacccattc
     1741 tactgggcgg gagtgatcct catccgaggc gcccgtgccg actgagccgc gggaggctag
     1801 cggtaggact ccttgtattt cgcagccaga ctctgcgcct tcacgcactc accgcgctgg
     1861 gccaggatct gaatttccag gatttcctgg tgtcggccca cgttcttggc gcgtttggct
     1921 gcgtcgcgct cgccctgcga ccagtaatac agctcccggc atgccgccgg gtcgaactgc
     1981 accacggggt tgcgcgcctt tcccagcccg cgttcgaaca attccatgct gtaatagccg
     2041 acgctcatga cgcccgatct tgcctcgccg gaggcaaagg tcagcgccag ggtgttcacg
     2101 tcgccgaagt attccaccag ctttccttcg cggttcgtcc ctttctcctc gggtgtctcc
     2161 ggcaatccga gcgagcggag cagggcggac cgggagaccg gtttcaggaa ccggcgctcg
     2221 atccggtcca cgaccgcatc ggcgcgaaac tccaccagga tgcttccggg cccgtctgcg
     2281 agcgagtact cgaaaagcgt cgcgctcacc gcccggaccg gctggcccag cgtgcgctcg
     2341 acgtcagcgc gggtgctctt gccgggctgg agccccttgt aggtgctgac cgccgccgcg
     2401 gggactccac ccagcagcag taccagcagc agcgggagcg cgcgcatcta ccgcgggcaa
     2461 ccgctcacgg aggcattcca caacccgtac caggtgttgc cgccctgcca gcggcctttc
     2521 atcccgatgc cgatggggaa gatccgtgac tccgtgatgc tcttgcataa ggcagcctgg
     2581 gcttcgtcct ggctggcgaa ggggccgagc agcttcctgt agctgatcag atcggtcggc
     2641 ttgcaaccaa tcccgccccc gacaaacccg cagcgggtgc tctgcttgat cccatcctcg
     2701 tcgcccacgt agaggccgtt ggaagcgttg gtgagcagga acacgtagta gcgcggcacc
     2761 ttcaccgcac cggtgttctt gctgaggtcg ccggggaccg agtcggtcgg ctcgtcagtt
     2821 gtcgggtcga actccgccag gacgatttcc tctgcgagag cgtcgagatc tttctcgagc
     2881 tggaaatcct cgactttgca tgccagggcc tcgagcagag tcggctcccg gagctccacg
     2941 gacgccacac tcgggtccca ggactcgagc tgcgatggtc cgcccaccag gactcgcggc
     3001 tcctcgatga aggcgccgag ctcgctggtc cgggtatcga tctcggtaat tgcggtggcg
     3061 gcatcggtga agcatttgta ggtgtccagg tcggctttgg cggatttcag cgcgtcggcc
     3121 agctgcttct tgcgttcgca caaattcttg tagttgagat tgcgtgccct gagcacggcc
     3181 accagcgcgg ccttctcccg cagcaacacc actttgtcag ctgccagccg tgtgcgctca
     3241 tcgcccaggg ccttggccgt ggccgggtct ttagtggtct gccaggcttt cacccgatct
     3301 tcccaggtct tcttttgctc gagaaaggtc ttcttgtggg tctggtatgg gtcgttcgca
     3361 gctctcaccg ccgtccaatc gaggagaaaa gtcctggtcg cggccaggtt ggtgttcgcc
     3421 gcctgcagca tcagattctt gtccgtgacc gccgcgtcgt aggcgggttt gaggttggtc
     3481 accgcgtcgc ggttggcctc gatttcgatg atcttgttca cctcgtcgag cattgcgtcg
     3541 gcaccctcct tcattactcg gctgtttttc acgtccgctt ctttcctggt caccaggtca
     3601 ccgagcaact tttgctcatc gaccaaccgt tgccgttccg tttcgagcga cttgtccgct
     3661 tccagcttcg tgagctcctc ctgccggtcc cggatcgcat tcccgggatc cagttgttcc
     3721 agctcggtgc gcttggtgcc aacttttttc aaaccgtcct gcagctcctt gtggcagtct
     3781 tgcacggcag tgcgcagagc cgtcaggtcg gcggccatct gatcctcaga gttcttgatc
     3841 gcctgcaggc tggccgtccg cgacccggct gcgttggggg aattctggtc gacgttctgg
     3901 tagtcgttca ggtgcttttc ccgtatcgcc tgctggcgcg ccagggctcg gtttttctcg
     3961 atcactggct ggcattgcat tgcggtggtc acaggggggg ggctgttcgt gggattgatc
     4021 gcccctccgc cagtcagccc gttcttaagc ttctgccacc acccgagctc gggcggctga
     4081 ttggcctggt a
//
LOCUS       DASRJQ010000300         2134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3461377, whole genome shotgun sequence.
ACCESSION   DASRJQ010000300 DASRJQ010000000
VERSION     DASRJQ010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3461377"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /locus_tag="VFB89_06720"
     CDS             <1..1154
                     /locus_tag="VFB89_06720"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HZM27031.1"
                     /translation="GRPACHNLPATRTAPGGLNDVPHDSAIVGFNHRGWLSQVVLALL
                     VAAGTLLPMSATPVSAHVRLVETVPTPESFLNSAPTEVRLTFGGFLQAAGNSVTVSTS
                     GGDANRAGAVQLDAFSRIITTPLQGPLPNGLYRVTWVAVAADGHAERGSFTFTLRAGA
                     EPPTMQLDRDVVDVGEPLVVTGSGFKASDAVAITAGADRHAVETVYADAEGRISQTVR
                     LPNTVPFGSQRIMAADSDGAAATATVQVIWGGWPPVRIQIEPRSLPQDLLLDVTVLNR
                     SGYDMGVDTVRIPVPPGTSFVAASTGGRLTPEGAVVWETLRLRPEETLGPFTVHLDAR
                     ALPAGTPVQASAEVLLRHPFEPSPASEEMPSFQTPVRSEPITARTGGRD"
     gene            complement(1251..2030)
                     /locus_tag="VFB89_06725"
     CDS             complement(1251..2030)
                     /locus_tag="VFB89_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZM27032.1"
                     /translation="MAARSLPPISLPSPKERRRLLLVDSDAPTVTNLRHALESDGYQV
                     IVAENASSARAALRDSWIDLAILDLMLPDSDGLVLCSDIKSAYGVPIIVYSGTRRRRD
                     AVLALRLGADDFVAKPFDLEDLEARIGAVLRRTVNTQARTGPSEPEYYQVGNLVVDRL
                     HRRAALSGEEIRLTPTEYRLLSALASQPDEVFSREDLTQLAWGWADANRSRTVDTHIR
                     RLRVKLQSASVPPPPIICVRGTGYKISTRESNTDADMSSVA"
BASE COUNT          380 a          674 c          705 g          375 t
ORIGIN      
        1 gcgggcggcc ggcctgtcat aatctgccgg cgaccagaac agctccgggc ggcttgaacg
       61 acgtgccaca cgactcagcg atcgtaggct tcaatcatcg agggtggctc agtcaggtag
      121 tcctggcact gcttgtcgcg gccggcacac tgctccccat gagcgcaacg cccgtttccg
      181 cacacgtgcg gttggttgag acggtcccca cgcccgagag cttcctcaac tcggcaccga
      241 ccgaggtccg gctcacgttc ggcggcttcc tccaggcggc ggggaacagc gtgacggtaa
      301 gcacgtcggg cggcgacgcc aatcgcgcag gtgcggttca gctcgacgcg ttcagcagaa
      361 tcattacgac tccgctgcaa ggtccacttc caaacgggct ctacagggtc acctgggtcg
      421 cagtggcggc cgatgggcac gccgagcggg gaagcttcac gttcacgctg cgggccgggg
      481 ctgagccgcc caccatgcag ctcgatcgcg acgtggtcga cgtcggcgag ccgctcgtgg
      541 tgacgggcag tggattcaaa gccagcgatg cggtggcgat taccgcaggt gcagaccgcc
      601 atgcggtcga gacggtctac gcggacgccg agggacgcat ctcccagacc gtgcgacttc
      661 ccaataccgt tccgttcggt tcgcagcgca tcatggcggc ggatagcgac ggcgcggccg
      721 caaccgctac cgtccaggtc atatggggcg gctggccgcc ggtgcggatc cagatcgagc
      781 cccgctcact tccccaggac ctcctgcttg acgtgaccgt gctgaatcgt tccggctacg
      841 acatgggcgt tgacaccgtg cgcatccccg tgccgcccgg caccagcttc gtggccgcga
      901 gcacgggagg gagacttacc cccgagggag cagtggtctg ggaaacgcta cggctgcggc
      961 cggaggagac actcgggccc tttaccgtgc acctggatgc gcgggctttg ccagcgggca
     1021 caccggtcca ggcctccgcg gaggttctgc tgcgccaccc gttcgagcca tcgccagcga
     1081 gcgaggagat gccaagcttt cagaccccgg tgcgctctga gccgataacg gcgcggaccg
     1141 gcggccgaga ctgagaagcg ccgaccaaaa tggtcggcgc ttctcacaaa cgtcctgtgt
     1201 agacgggggt cagacgtcgg cctgacccca ccggcggatc gcgcaaggga tcacgccact
     1261 gatgacatgt cggcgtcggt gttcgattcc cgcgtactga tcttgtagcc cgtgccacgc
     1321 acacagatga tcggtggcgg gggtacgctc gcactctgga gcttgacccg aagccgcctg
     1381 atgtgggtgt caaccgtgcg gctgcggttc gcgtccgccc agccccatgc aagctgggtc
     1441 aggtcttctc gtgagaacac ttcgtcaggt tggctggcaa gcgcgctgag cagacggtac
     1501 tccgtcggcg tgaggcggat ctcctcgccg ctcagggcag cgcgtcgatg gagacgatcg
     1561 acgacgagat tgccgacctg gtaatactca ggctccgatg gtcccgtgcg ggcttgcgtg
     1621 ttaaccgtac gccgcaacac ggcaccgatg cgcgcctcga ggtcttcgag gtcgaacggc
     1681 tttgcaacga aatcgtcagc accgagccga agggcgagga cggcatcgcg tcgacgccgg
     1741 gttccgctat acacgatgat gggcacgcca taggcgctct ttatgtccga acatagcaca
     1801 agtccgtcac tatctggcaa cattagatcg agaatcgcca gatcgatcca cgaatcgcgt
     1861 agcgccgcgc gggcgctgct ggcattctcg gcgacgatga cctggtaacc gtcggactca
     1921 agggcatggc gaaggtttgt aacggtcggt gcatccgagt ccacgagcag gaggcgccgc
     1981 cgctctttcg gggacggaag cgatatcggg ggcaagctgc gagcggccat tgttctcctc
     2041 tgactgacca ccggtcgcgt ttggcgaacg gctgtgaaac ttgtattcta ttttaccgtt
     2101 atggtaagta atcgtgcagg aacatgtgtc cggg
//
LOCUS       DASRJQ010000301         3442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2151395, whole genome shotgun sequence.
ACCESSION   DASRJQ010000301 DASRJQ010000000
VERSION     DASRJQ010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2151395"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..794
                     /locus_tag="VFB89_06730"
     CDS             69..794
                     /locus_tag="VFB89_06730"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HZM27033.1"
                     /translation="MTPHDWYVENRTAFVVRTLELREERLFAEHLPRCDECRREVARL
                     ERDLGALPMAAMPVAPRPGLVHHLADAVLNRRVWWRRAAPALAAAAVLMLAIGFGLRE
                     RAHQSVLATTLAKRERELSALRDTLSIWRESQRVVQRDIAMGNHKGGLLIFDDPTTHR
                     WNVVMHGLPAAPTDSVYQFWFITRSGMVRSVELRCEANRPAFATLDMPKTPGPVMGAA
                     LTIEPAVSHSPEPRGLELAHVQF"
     gene            complement(804..2429)
                     /gene="dacB"
                     /locus_tag="VFB89_06735"
     CDS             complement(804..2429)
                     /gene="dacB"
                     /locus_tag="VFB89_06735"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HZM27034.1"
                     /translation="MPIGYRSRPLGHTRLKGSLGQLGLLLLVGHSVPAWAQATGQDWL
                     DQKMGAWYRVASRAAPGHWGVAVADLDGQVLWSINPDDPLIPASTVKVFTTGFARSVL
                     GSSARRPTRVVGSGRVDPNTGQWIGSWSLELNGDPSFERGPGAGPTLYDLALQLSSSG
                     IRRLVGPLNVQSSDGAADAIFPSVWSRHHFGRLFAPLIGPITINENVVWVVVQPGSRP
                     GARPRVVAESPEGIGSLVTIKATTRSGRRSSLALRARPEGGWIVTGAIGVRARPRRLA
                     AVASDPKVVLAAAWTRALQRAGITWNPASFVGAPPTAAPRVLAEVSSPPLDSLASEIN
                     RRSLNYGAELLLQWAGGRETPASRLMQHVQQVTGTSAGLELVDGSGLSYDDRVTARTF
                     IAYLAKFPATPEGRNFPQLLPANGTGTLYHLASGLPSEGVVRAKTGTLAQVANVVGYL
                     GRDDGVLLVALLYNGSRPWAARKVEWKLFRDLGAHGVVIPSDTLPESVLPHLGGENES
                     DFVPAWKSDSTDTTVVDDDSLPALTTPDSTLQH"
     gene            complement(2433..>3442)
                     /locus_tag="VFB89_06740"
     CDS             complement(2433..>3442)
                     /locus_tag="VFB89_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZM27035.1"
                     /translation="KFFELGLMGIGVPDRFGGAGGTIFMATLAIEELARVDASAAIYV
                     DVHNTLVCNALDRWGNEEQKARYFPRLNTDLLGAFALSEPASGSDAFALETKATPSGS
                     DWALTGRKFWITNGGEAGFFVVFANTDFSKGYKGITGFLVERDFPGFSIGKKENKLGI
                     RASSTVELILEECQVPQANVLGPVGQGYKIAIETLNEGRIGIGAQMIGVAAGALEAAT
                     QYVKERKQFGKSISEFQGVQFQLAQMATELEAARLTVYNAARLKDSGRPFAREAAMAK
                     LFSSQVADRTTSRCLELFGGYGYSKEYPAEKYYRDAKIGTIYEGTTNMQLQTIAKMML
                     R"
BASE COUNT          584 a         1122 c         1117 g          619 t
ORIGIN      
        1 aagacgaggc tgcgcctcgc ctttgacaag ctgcgggtgg cgctcgcacc gatcggggat
       61 tgggttcaat gaccccccac gactggtacg tggagaatcg cactgcgttt gtcgtgcgta
      121 cgctcgagct ccgggaggag cggctcttcg ccgagcacct gcctcgctgc gacgaatgcc
      181 gccgggaggt cgctcggctc gagcgggatc tgggcgcttt acccatggcg gcgatgccgg
      241 tcgcgccgcg gcccggcttg gtccatcatt tggcagacgc ggtcctcaac cggcgagtgt
      301 ggtggcgccg ggcagcgccg gcattggcag ccgctgcggt tctcatgctg gcgattggct
      361 tcgggctacg cgagcgagcg caccaatccg tgctcgcgac caccctggcc aaacgggagc
      421 gcgagctgtc ggcgctccgt gataccctgt cgatctggcg ggaatcgcag cgggtggtgc
      481 agcgggatat cgcgatgggg aaccacaagg gtgggctgct gatcttcgac gaccccacca
      541 ctcacagatg gaacgtcgtc atgcacgggc tgccggcagc cccgaccgac tccgtctacc
      601 agttctggtt catcacgcgg agcgggatgg tgcgttcagt agagcttcgt tgcgaggcca
      661 atcggccggc tttcgccacg ctggacatgc ccaagacgcc ggggcctgtc atgggggccg
      721 ccctgacgat cgagccggcg gtaagtcatt ctcccgagcc gcggggactc gagctggcgc
      781 acgtgcagtt ctagaggcaa gactcagtgc tggagggtcg agtccggcgt cgtcaaggcg
      841 gggagagagt cgtcgtctac gacggtggtg tctgtcgaat cggacttcca ggccggaacg
      901 aaatcgcttt cgttttcgcc ccccagatgc ggaaggaccg actccggcaa ggtgtcgctc
      961 ggaatgacga cgccatgcgc accgagatcg cggaacagct tccattccac tttgcgcgcc
     1021 gcccagggtc gggagccgtt gtagagcagg gccaccagca gcacgccgtc atcccgaccg
     1081 agatagccga ccacgttcgc gacctgtgcg agcgtccccg tcttggcccg caccacgcct
     1141 tcgctcggca gcccggaggc gagatggtag agggtaccag tgccgttggc cggcagtaac
     1201 tgagggaagt tgcgaccctc gggcgtcgcg gggaacttcg cgaggtatgc gatgaatgtc
     1261 cgcgcggtca cccggtcatc gtaggacaat ccgctgccgt ccaccagctc gagcccagcg
     1321 ctggttccgg ttacctgctg gacgtgctgc atgagacgcg acgccggggt ctcacgtcct
     1381 cccgcccatt ggagcaacag ctccgctccg taattcaggc tgcggcgatt gatctcgctg
     1441 gcgagcgaat cgagcggggg ggacgagact tctgccagga cgcggggtgc ggcggtaggc
     1501 ggcgcgccga cgaacgacgc cggattccag gtaatgcctg ccctttgcag cgcgcgagtc
     1561 cacgcggccg caaggactac cttggggtcc gaggcgacag ccgcgagccg gcgcgggcgg
     1621 gcccgcacgc cgattgcccc ggtcacgatc cacccgcctt cggggcgagc acgaagggcg
     1681 aggctcgagc gtcgcccgga gcgggtcgtt gctttgatgg ttaccagcga cccgattccc
     1741 tcgggcgatt cggcgaccac gcggggacgg gcccctggcc gagatcccgg ctgcaccacg
     1801 acccatacaa cattctcgtt gattgtgatt gggccgatca acggagcgaa cagccgcccg
     1861 aaatggtggc gggaccagac gctcgggaag attgcatcgg ctgctccgtc cgagctctga
     1921 acattgagcg gaccgaccag ccttcggatc cccgagctgg agagctggag tgctagatcg
     1981 tagagcgtgg ggcccgcgcc ggggccgcgc tcgaaggagg ggtcgccgtt gagctccagg
     2041 ctccagctcc caatccactg gccggtattc ggatcgaccc tgcccgagcc gaccacgcgg
     2101 gtcggccgcc gggcactgct accaagaacc gaccgggcga acccggtggt gaagaccttg
     2161 accgtcgacg ccgggatcag cggatcgtca gggttgatgc tccacagcac ctgcccgtct
     2221 agatcggcga ccgcgacgcc ccagtggcct ggcgccgccc tggaggccac gcggtaccag
     2281 gcacccattt tctggtcaag ccaatcctgg cctgttgcct gggcccaagc gggtacactg
     2341 tggcccacca gcaacaggag cccgagttgc cccaggctcc ccttcagtcg cgtatggcca
     2401 agtggccgtg agcgatagcc gatcggcacc ccttacctca gcatcatttt cgcaatggtc
     2461 tgcaactgca tgttggtagt cccctcatag atcgtgccga tcttggcgtc ccggtaatac
     2521 ttctctgccg gatattcctt agaatagccg taaccgccga atagctcgag gcatcgggag
     2581 gtcgtccggt cggcgacttg ggagctgaaa agcttcgcca tggccgcctc gcgggcgaag
     2641 ggccggccac tgtccttgag cctcgccgca ttgtagaccg tgagtcgggc tgcctccaac
     2701 tccgtcgcca tctgcgccag ctggaactgc accccctgga actcggagat cgatttgccg
     2761 aattgctttc gttccttgac gtactgcgtc gcggcctcga gcgctccggc cgcgactccg
     2821 atcatctgcg ctccgattcc gattcggccc tcgttcagag tctcgatcgc gatcttgtaa
     2881 ccctgtccca ccggtcccaa cacgttcgct tggggaacct ggcactcttc caggatgagc
     2941 tcgaccgtgc tcgatgctcg aatgccgagc ttgttttcct tcttcccgat cgaaaagccg
     3001 gggaagtccc gctctacgag aaatccggtg atgcctttgt atcctttgga gaagtcggta
     3061 ttggcgaaga ccacgaaaaa gccggcttca ccgccgttgg tgatccagaa tttgcgaccg
     3121 gtgagagccc agtcgcttcc ggatggggtt gccttggtct cgagcgcaaa tgcgtcgctt
     3181 ccgctcgccg gctccgacag cgcgaaggcg ccgaggaggt cagtgttgag ccgcgggaag
     3241 taccgggctt tctgctcctc attgccccag cgatcgagcg cgttgcacac cagcgtgttg
     3301 tgaacgtcca cgtagatggc ggccgaggca tccacccggg ccagctcctc gatggcaagt
     3361 gtcgccatga aaatcgtccc gcctgctccg ccgaaccgat caggcacacc gatgcccatc
     3421 aagcccagct cgaaaaactt cg
//
LOCUS       DASRJQ010000302         6573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2605439, whole genome shotgun sequence.
ACCESSION   DASRJQ010000302 DASRJQ010000000
VERSION     DASRJQ010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6573
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2605439"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VFB89_06745"
     CDS             complement(<1..231)
                     /locus_tag="VFB89_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27036.1"
                     /translation="MRIGVMLRGVDKKGGSGIYAQNLVPELLRQGSDVEWVLIYSNAS
                     QLGSFELPSRAEEVVVPSVHPVSWDQLAVPRFA"
     gene            complement(609..2486)
                     /locus_tag="VFB89_06750"
     CDS             complement(609..2486)
                     /locus_tag="VFB89_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27037.1"
                     /translation="MWLVAMAVARGAEIAIAAVVGVAGAQLVTSRWAAARFARRLRSA
                     RLARIVGAKPEVERGVPVRFGLMHPGRCLRRWEAECLDQLVALPFVELSIVIVPGQAP
                     SVALRSPNWPTRLLSWMGRRGPWEPADGTRYLSDVLELHCRVVPSGAGYCIAPEDVTA
                     IRERKLDFLITFGPGPWSGGVLDAARWGLWAVRYPGQRTPEDIPPGFWEACHEEATTG
                     AALVALSDEPGGDRILKQGIMRTPGESYAGNAAEILSEVSSWPAQVCRELSGGGGSLA
                     DAHRLRVRKPEPPPRGRDLLRLVLSMAHERWREFARKNVYGDLWNVGVVDAPIASFLD
                     PDFRPAVRWFPALPPGEFRADPFGLVHEGSLTILCEHFAYRDDRGFIVALDANASAGI
                     VNCRIAMAPAFHLSYPYLIEHDGQIFCVPESHQAGEVALYRADEFPTRWTKAATLLAG
                     VPALDATIFPHGDRWWLTCTMQGPNERSHLYVYHSPGLLGPWTAHLANPVKTDITSAR
                     PGGTPFVHQGTLYRPSQDSSRTYGGRVVINRVTTLTPGRFEEECATVVAPFAGSLYPD
                     GVHTLSAAGPVTLLDGKRIVLIPTAIGMGGLRPYLLRRLRHILRTTDEVLDTVGHRLR
                     V"
     gene            complement(2527..2685)
                     /locus_tag="VFB89_06755"
     CDS             complement(2527..2685)
                     /locus_tag="VFB89_06755"
                     /inference="COORDINATES: protein motif:HMM:NF039953.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HZM27038.1"
                     /translation="MGRSGMAGELWAYMRVRKKWWLLPVIAVLVLVGALLVLAHGSVL
                     APFIYTVF"
     gene            complement(2707..3111)
                     /locus_tag="VFB89_06760"
     CDS             complement(2707..3111)
                     /locus_tag="VFB89_06760"
                     /inference="COORDINATES: protein motif:HMM:NF039992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HZM27039.1"
                     /translation="MAGSVPARLSAAEGRKFGLTVGIAFLVLATLLSWRGRPYGALGA
                     AVVGGLLLIAGLAIPTRLAPVRTGWMRLAEAISRVTTPVFMAIVYFGVITPTGLLLRL
                     GRHRPLVRQRSTGGVWLTRATGDRRSDLTRQF"
     gene            complement(3084..4910)
                     /locus_tag="VFB89_06765"
     CDS             complement(3084..4910)
                     /locus_tag="VFB89_06765"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HZM27040.1"
                     /translation="MPGPTHILGISAFYHDSAACLLRDGEIVAAAQEERFSRKKGDAS
                     MPLAAVGYCLTAGGIAPRDLHCVAFYDKPLLKFERILETYLGIAPRGFTSFLKAGPLW
                     VKEKLFIDREIRQALNYDGEVLYAEHHESHAASAFFPSPFEEAAILTMDGVGEWATAS
                     IGVGRSDEIELLKELHWPDSLGLLYSAFTYYTGFKVNSGEYKVMGLAPYGEPKYVDLI
                     YRELMDLREDGSFTLNQRYFNYLGGLTMTSAAFDQLIGGPPREPESRLTQKEMDLARS
                     IQAVTEEVMLRMARTAHRETGLDNLCLAGGVALNCVGNGRLLREGPFRQLWIQPAAGD
                     AGGAVGAAQLAWHRQLHRPRTPSKGDGMKGAYLGPEFPADEIEAFLKKQGAPYERPAR
                     ADLLLAVAKQLADGQVVGWFNGRMEFGPRALGARSILGDPRNPRMQAQMNLKIKFREG
                     FRPFAPSVLRERVSDYFELDGDSPYMLLVAPVRKDRQIPMTVEQRQLWGIDQLNVLRS
                     DIPAVTHIDYSARIQTVARETNRDYYDLIKAFEALTGCGVLVNTSFNVRGEPIVCTPQ
                     DAYRCFMRTEIDTLVLGPFLVAKSRQPEWKETDQWQVQYQLD"
     gene            5265..6551
                     /locus_tag="VFB89_06770"
     CDS             5265..6551
                     /locus_tag="VFB89_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HZM27041.1"
                     /translation="MDPSRSVGASPPRKGRGWGWVSIGSFALLALLVAFRSRVVVKRS
                     IRRTVAAAKHRLYHERPGVAWETARPEKEGLSSAVLDSLCRVLAANGTHAFLVVRGNH
                     IVFEWYSTKSGPNVPELTTAMAKAATANIALLTAITDGRISLDDSASKYIPGWRNDTL
                     RSRIRIGDLAAHQSGLDDVDFGLAGKGGLPDWKEQYFHHPDQRFGMAIGTVPLIDAPG
                     ARSSYSGVGYYALAYALTTSLKQANQHDIKTLLRERIMHPLGIQDDNWRLSYGASYQV
                     DGMTLYAMGSGAAYTARAVARIGELMLDHGRFGNQQLIDSAVITKALQPATAPIPEDS
                     STYPVTPAAAGGGWWLNIRGAWPDVPRDAFAGMGGQQEALLVVPSLDLVMVRLGAEMV
                     PGDDQFAQAFRDRLFDPLMHAVIGASSRMRQSSSAH"
BASE COUNT         1288 a         2120 c         2051 g         1114 t
ORIGIN      
        1 cgcaaagcgc ggcaccgcca gctggtccca ggacaccggg tggacgcttg ggacgactac
       61 ctcctcggcg cggctgggta gctcgaagct cccgagctgc gacgcattcg aatagatgag
      121 cacccactcg acgtcgctgc cttgccgcag gagctcaggc acgaggttct gggcatagat
      181 gccgcttccc cccttcttat cgaccccacg cagcatgacg ccgatacgca tggcgctact
      241 ccggctcaaa tgttggagaa agccaaccct ccgcgccaac cgtagatccg gggtgccgtc
      301 tctaccccag gaaccctgct gactggccga atgcccaagt aagctcgtct tcccgagggc
      361 taagaaggcc gccatcgcag gctcgccctc gactgatcgg ccgagggcga gcatagcgaa
      421 gactggcacg aacccgcaag aaggagagtc acaaagggcc caccaaggct aagtagccgt
      481 ctccatcttg tggcgggtgc tgcaagaagt ctccgacgaa ttgcagtttc ctgcatacgt
      541 cgctggctac ctgggagaat catgcactga gagagacggt gttcagtcca ggaccgcgtt
      601 tcgtatcatc agacacgaag gcggtgaccc acggtgtcca gcacctcgtc agttgttctc
      661 aagatgtggc ggagccggcg caagaggtac ggccgcaagc cccccatacc gatggcagtc
      721 ggaatcagta cgatccgctt cccatcgagc agggtgaccg gacccgcagc ggacagggta
      781 tgaaccccgt caggatacag gctgcccgcg aacggcgcta ctacggttgc gcactcttcc
      841 tcgaaccggc ctggcgtgag cgtcgtcact cggttgatga ccacgcgacc cccgtaggtg
      901 cgagaggagt cctgcgaggg acgatagagg gtaccctggt gcacgaacgg ggtcccaccc
      961 ggccgggccg aggtgatgtc ggtcttcacg gggttcgcga gatgggcggt ccacgggccc
     1021 aggagacccg gcgaatgata gacgtaaaga tgtgaccgct cgttcggtcc ctgcatcgtg
     1081 catgtgagcc accagcggtc cccgtgagga aagatcgtcg cgtcgagggc gggtacgccg
     1141 gcaagtaacg tggcggcctt ggtccagcgc gtcggaaatt catcagcccg gtacagggcg
     1201 acctcgcctg cctgatggga ttcgggaacg cagaagatct gcccgtcgtg ctcgatgaga
     1261 tagggatacg acaagtgaaa cgccggagcc atggcgattc ggcagtttac tatgcctgcc
     1321 gacgcgttgg catcgagcgc tacgatgaat ccgcggtcgt cccggtaggc gaagtgctcg
     1381 caaaggatcg taagactgcc ctcgtgcacg agcccgaacg ggtcagctcg gaactcccca
     1441 ggtgggagcg cgggaaacca tcggacggca gggcgaaagt cggggtcgag aaatgacgcg
     1501 atcggagcgt cgacgacacc cacgttccaa aggtccccat atacgttctt ccttgcgaac
     1561 tctcgccatc gttcgtgggc catagacagg acgagacgaa gaagatcccg gcctcgcggc
     1621 ggcggttccg gcttgcgcac gcgcagccgg tgggcatctg ccaggctgcc gccacctccg
     1681 ctgagctcgc ggcagacttg cgctggccag ctgctcacct cggacaatat ctcggcggca
     1741 tttccggcat acgattcacc tggggtcctc atgatacctt gcttgagaat ccggtcgccg
     1801 ccaggttcgt cgctcagggc gaccagtgcg gcgccggtgg tcgcctcctc gtgacaagcc
     1861 tcccagaacc ccggcgggat atcctccggg gtccgctggc cggggtagcg gaccgcccag
     1921 agtccccacc gcgccgcgtc gagcacccca ccactccagg gccccgggcc aaaggtgata
     1981 aggaaatcga gcttgcgctc gcggatggcg gttacgtctt cgggcgcgat gcaatagccg
     2041 gcgcccgaag ggacgacgcg acaatgaagc tcgagtacgt cactgaggta cctggtgccg
     2101 tcggcaggtt cccacggccc gcggcgcccc atccacgaga gcaagcgggt tggccagttt
     2161 ggggaccgca gcgccactga cggagcttgc cccggcacga tgacgatgga gagctccacg
     2221 aaggggagcg cgaccagctg atcgaggcac tcggcctccc aacggcgaag gcaccgtccc
     2281 ggatgcatta gcccgaatcg gacgggcact ccccgctcca cctccggctt cgcgccaacg
     2341 atacgcgcca gacgagccga tcgcagacgg cgcgcgaacc tggccgctgc ccaccggctc
     2401 gtcaccagtt gcgcgcctgc cacgccaacg acggcggcga tggcgatctc cgcaccgcgg
     2461 gctacggcca ttgcaaccag ccacatcggg acgtgacgcc gagcgaacgg cgctagaagg
     2521 ctgtcgttag aagaccgtat agataaaggg cgccagtacc gacccgtgtg ccaacacgag
     2581 cagcgcaccc accaacacca gcacggcgat caccggcagc agccaccact tcttgcggac
     2641 tctcatgtac gcccacaact caccggccat accagacctg cccatgatgc ctcccgagaa
     2701 ttctccttag aactgccggg tgaggtcgct gcgacgatcg cccgtcgccc tggtgagcca
     2761 gaccccccct gttgaacgct gccggaccag cggccggtgg cgaccgaggc ggagcagcag
     2821 ccctgtcggc gtgatgaccc cgaagtacac gatcgccata aacaccggtg tcgtgacacg
     2881 ggatatagcc tccgcgagac gcatccatcc ggtacgaacc ggcgccaacc gtgtggggat
     2941 ggccaacccg gcgatgagca gcaaccctcc caccaccgct gcccctaaag ctccgtacgg
     3001 cctccctcgc cacgagagca gcgtagccag gactaggaac gcaatgccga ccgtcaggcc
     3061 aaacttgcgc ccctccgcgg cgctcagtct agctggtact gaacctgcca ctggtcggtc
     3121 tccttccact cgggctggcg gctcttggcc accaggaacg gtccaagaac cagagtatcg
     3181 atctccgtgc gcatgaagca gcggtacgca tcctggggcg tgcagacgat gggctcaccc
     3241 cgaacgttga aagacgtgtt gaccaataca ccgcaaccgg tcagtgcttc gaatgccttg
     3301 atgagatcgt agtagtcccg gttagtctcc cgcgccacgg tctggatcct cgccgagtag
     3361 tcgatatggg taacagccgg aatgtccgaa cggagtacgt tcagctggtc gataccccag
     3421 agttggcgct gctcgacggt cattgggatt tgccggtctt tccggaccgg agcgaccaac
     3481 agcatgtaag gggaatcgcc atccagctcg aaatagtcgc tgacccgctc ccgcaagacg
     3541 ctgggcgcaa agggccggaa gccctcgcga aacttgatct tgaggttcat ttgcgcctgc
     3601 atccgcgggt ttcgcggatc cccgagaata ctgcgcgcgc cgagagcgcg tgggccgaat
     3661 tccatccggc cgttgaacca gccgacaact tggccatcgg caagctgctt cgccacggcg
     3721 agcaacagat ctgcccgggc cggccgctcg tagggcgctc cttgcttctt gaggaacgcc
     3781 tcgatctcat cggcgggaaa ttccgggcca aggtaggcac ccttcatccc gtcgcccttg
     3841 ctcggcgtgc ggggccggtg cagttgccga tgccaagcga gctgcgcggc tcccaccgca
     3901 ccgccagcat cgccggcagc cggctggatc cagagctgcc ggaacgggcc ttcccgaagc
     3961 aggcgaccat tgcccacgca gttgagagca acgccaccgg caaggcagag gttgtccagc
     4021 cctgtttccc ggtgcgccgt ccgagccatc cgaagcatga cctcctccgt cacggcctgg
     4081 atcgaccgcg cgagatccat ctccttctgc gtgagccgcg actccggctc ccgtggcgga
     4141 cctccgatga gctggtcgaa agcggcactg gtcatcgtca ggccgcccag atagttgaag
     4201 tatctctgat tgagggtgaa cgacccgtcc tcacgaaggt ccatgagctc gcggtaaatc
     4261 aggtccacat acttcggctc cccatacggg gcgaggccca tgaccttata ctctcctgaa
     4321 ttgaccttga aaccggtgta gtaggtgaag gcggaataaa gtaggccgag ggagtcgggc
     4381 cagtggagct ccttgagcag ctcgatttca tcgctgcgac cgaccccgat tgacgccgtg
     4441 gcccactcac ccacaccgtc catggtcagg atcgctgcct cctcgaaggg cgatgggaag
     4501 aaggcgctag cggcatgaga ctcatggtgc tcagcgtaaa ggacctcgcc gtcgtagttc
     4561 agcgcctgcc gaatctcgcg atcaatgaac agcttctctt tgacccaaag cggcccggct
     4621 ttgaggaagg acgtgaagcc gcgcggagcg atgccgaggt atgtttcgag aatgcgctcg
     4681 aacttgagca gcggcttgtc atagaatgcg acgcagtgaa ggtctcgggg agcgatgccc
     4741 ccagccgtga ggcaatatcc caccgccgcc agtggcatgg aggcgtcgcc tttcttacgg
     4801 ctgaagcgct cctcctgagc ggcagccacg atctcgccgt cgcgaagcaa acaagccgcg
     4861 ctgtcgtggt agaacgcgga tatgccgagg atgtgggtcg gtccaggcat cgggcctctc
     4921 ggggtggtcg tgctccgctc ggtgcaaatc cgatgcggac gtttggaccc tgccgctgct
     4981 ccttgccgga cgcctcgcac ggtggcgatc gtagcgcacc agcaacgggt gtcgcgatct
     5041 cgccacggaa ccagggggcg tcgagtctct cgtccaaggc cgagggtccc ggttacctgg
     5101 tagactgcat aaaattgcag cacccggcct gcgagatcaa tcaagcgtac cggcggtgct
     5161 gacgggcgga caattgaggc atatgcaaga aatggagtca gctacaaggg cacaaacgtt
     5221 gcgcccccac cggagcctgc ccccaaaccc cttaggaact gatcatggat ccatcgagat
     5281 cggtcggcgc aagcccgcct cgcaagggcc gaggatgggg atgggtcagc ataggctcgt
     5341 tcgcactgct tgcacttctc gtcgcatttc gcagccgcgt ggtggtcaag cgttctatcc
     5401 ggcggacagt cgccgcggcc aaacacaggc tttaccatga gcggccaggt gtggcctggg
     5461 aaaccgcccg ccctgagaag gagggtttaa gcagcgcggt gctcgacagc ctgtgccgcg
     5521 tactcgccgc aaacggcact cacgccttcc ttgtggtgcg cggcaatcac atcgtcttcg
     5581 agtggtattc gaccaagtcg ggtccaaacg tgcctgagct aacaacggcg atggccaagg
     5641 ctgcgaccgc gaacatcgcg ctactcactg ccatcaccga tgggcggatc agtctcgacg
     5701 attcggcttc gaaatacatc ccgggatgga ggaacgacac actccggtct cggatccgaa
     5761 tcggtgacct cgccgctcat cagtctgggc tggacgacgt ggacttcgga ctcgccggca
     5821 agggcgggct cccggactgg aaggagcagt actttcatca cccagaccaa cgtttcggca
     5881 tggcaatcgg tacggttccc ttgatcgatg caccgggcgc ccgctccagt tacagtggcg
     5941 tggggtacta cgcgctcgct tacgccttaa ccaccagcct gaagcaagcc aaccagcatg
     6001 atatcaagac cctgctgcgt gagcggatca tgcaccctct cggaattcag gacgacaact
     6061 ggcggctgag ctacggagcc tcgtaccaag ttgatgggat gactctctat gccatgggca
     6121 gcggcgccgc atacaccgcg cgcgccgtgg cccggattgg agagctcatg ttggatcatg
     6181 gccgctttgg taaccagcag ttgatagatt ccgcggtcat caccaaggcc ctccagcccg
     6241 ccacggcacc catcccagag gactccagca cctatccggt gacgccggcg gccgcgggtg
     6301 gcggatggtg gctgaacata cggggggctt ggcctgacgt tcccagagac gctttcgccg
     6361 ggatgggcgg ccagcaagaa gcgcttctgg tcgtgcctag tctcgacctc gtgatggtcc
     6421 ggctgggagc tgagatggta ccgggggacg atcaatttgc gcaagcgttt agggaccggt
     6481 tgttcgaccc gcttatgcac gcggtgatcg gcgcaagctc gcggatgcgg cagagttcga
     6541 gcgcccacta ggcgtgctct cggcctcggc atg
//
LOCUS       DASRJQ010000303         5565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1162909, whole genome shotgun sequence.
ACCESSION   DASRJQ010000303 DASRJQ010000000
VERSION     DASRJQ010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5565
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162909"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="VFB89_06775"
     CDS             <1..936
                     /locus_tag="VFB89_06775"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HZM27042.1"
                     /translation="SLADSPRAGDEGRAARYWAGRAWDRANDSLRARSRWSSLVERDP
                     TSYYAGLSERRLGLATWTPPAAPDSFVSVPSVDSAMDRAKLLVRLGMPDEAGWEYDRI
                     VRDAGRSVEGLLATADALRSAGYAYQGMRLARRALAAGVPADARLYRLLYPVVQRDAL
                     VAECAAQGLDPALVAALIRQESSFNPAATSSVGARGLMQVMPDVGRTVAGGLGFSEWD
                     PVLLWQPDVSISIGTVHLRDLVARYTDQTRILAAYNAGISRVDRWDDKLGVEDPEVFA
                     ERIPFVETRDYVRIIQRSRDLYRVLYDWKAGKKGG"
     gene            1051..2751
                     /locus_tag="VFB89_06780"
     CDS             1051..2751
                     /locus_tag="VFB89_06780"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZM27043.1"
                     /translation="MESSAAGQVETIPRTHSQRIGRPSLPPQRTLLTWLFVGRLVLAL
                     ATLVGASLVWTQRPDVSFISTITVVFALTFTGYGAWAVFVRGVIPSRAFLQIQALVDL
                     GLVTALVHLAGEPKSAFPALYVLVVAAYTMLLPLGAGVFTAVAASAMYLGDILLGQTA
                     RPDTALWIQFGVFNVVFLLVAVLSQRLREAGVEQESLETELARVRLEADDILRNIRSG
                     VLTVDGRGRLAFINPTAERMLRLQASSLIGQPVLEHLKARSSELWAAINSGIRAGRKI
                     SRGEGLVVSAQGRQFPIGLSTTVFQQESQETPSVTAIFTDISDLKQLQELHLRAERLE
                     AVAALSASLAHEIRNPLASIRSSVEQLSRAKYATSDERFLADLIVRESDRLSRLLNEF
                     LDFSRVRATEFVPVDLRDVALAVTRLVKEHPDCPAEAVLEVHGARTVLDGDADLLHRV
                     IANLVLNAVQAAKGSKVCVTVSVGEADGGADLPHGHLDRAVRLEIRDDGPGIPDEIRE
                     RVFEPFVSGRPGGSGLGLSIVQRAVEAHRGLVLVDSKPGAGTAFTVLLPVEWTPEEGA
                     "
     gene            2748..4124
                     /locus_tag="VFB89_06785"
     CDS             2748..4124
                     /locus_tag="VFB89_06785"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZM27044.1"
                     /translation="MSQKPSVLVVDDESGILDTLRILLRNEGFEVTTAQGGKAGLEQI
                     RSGSHDIILSDVRMPQVSGLDILQAAREQDPMTPVILMTAQASLQSAIAAVNSGAFHY
                     IQKPFSNDELVAILRRACEFRAVRVENKQLKQEIRRRERAGTTVTRPIGKSKRFVEVL
                     KLAEHVAPTDSTVLIQGESGTGKEVVARYIHNLSSRSDGPFLSINCGALPENLLESEL
                     FGHVKGSFTGAVRDKQGLFAAARGGSFFLDEVGEMPPSLQVKLLRVIQEREVIPVGAT
                     EAIPVDVRIIAATNRDLEEEVRRGNFRSDLFYRLNVIAVNLPALRERRDDLISLIELF
                     LENLGQETGGEAKALSQDALDAVTVYEWPGNVRELENALEHAVVLSKGSLIEAGALPE
                     RITKRKKEPLVAERSYKNPTLEVIERAYIMYVLQAEGGNKTRAAEVLGIDPSTLYRKL
                     SRYEGEAV"
     gene            4262..>5565
                     /locus_tag="VFB89_06790"
     CDS             4262..>5565
                     /locus_tag="VFB89_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27045.1"
                     /translation="MANWREILTASWWPNHNLYRPLTALTLAMNWAASGGNPTAFHVT
                     NIALHAAAAVLAYLLATRLLGVGGGALTAGLLFAVHPVHVEAIANLVGRAEVLATIFV
                     IVAVLLYGLDGRLAESGDDRSWKRYATAAGTLVATAFALASKESAFALPGLLLLLDWL
                     DASLRGRRGLGVERHWVLWLGAVAVAFAWLLWRANVVGDLTGKEAAPGVEDTGLVGRT
                     VAMLPVVLQYGRLLFFPARLSADYSPNFLVLGSWLTLRTLAGLLALIACGAVAWVAWR
                     RAPVVTFSLGWIGATILVVANILVPTGVILAERTLYLPSVGAVLLLGWIAGVAWTRRP
                     LATATVVGLLVAAGGARTMTRNPVWRNNATLFPQIVRDAPGSSHAAWVAGGMAEEAGN
                     LPEAERMYRQSLEIYPLAWNAWRDLGRVINAQGRYAEAGDAW"
BASE COUNT          898 a         1756 c         1875 g         1036 t
ORIGIN      
        1 tcgctggccg acagtccccg cgcaggtgat gaagggcgcg ccgctcgtta ctgggctggt
       61 cgtgcctggg atcgggcgaa cgattcgttg cgggcccgtt cccgctggag ctccctggtc
      121 gagcgggacc caacgtcgta ctacgccggg ctgagcgagc gccggctcgg cctggcgacc
      181 tggactcctc ccgctgcccc ggactccttt gtctcggtgc cgtcggtcga cagcgcgatg
      241 gatcgagcca agctgctcgt gcggctcggc atgccggacg aggcgggctg ggaatatgac
      301 cggatcgtgc gcgatgcagg tcgctcggtc gaaggactcc tcgccacggc cgatgcgtta
      361 cgaagcgccg gctacgccta tcagggaatg cggctcgcac gccgggctct cgcggccgga
      421 gtgcccgccg acgcccggct ttatcgcctg ctgtacccgg tagtgcagcg cgatgctctc
      481 gtcgccgagt gcgccgcgca aggcctcgat ccggctctgg tggcggcctt gatccggcag
      541 gagtcgtcgt tcaatcctgc ggcgacctct tccgtgggag cgcggggact catgcaggtc
      601 atgccggatg tcggaaggac ggtcgccggc gggctcggct tttccgagtg ggatccggta
      661 ctcctctggc aacccgacgt gagtatctcg attggcaccg tccatctgag ggacctggtg
      721 gcccgctaca ccgaccagac ccgcatcctg gccgcataca acgccggtat ctcgcgggtg
      781 gatcgttggg acgacaagct gggtgtggaa gacccggagg tgttcgccga gagaattcct
      841 ttcgtcgaga cccgggacta cgtccggatc attcaacgca gccgggatct gtaccgagtc
      901 ctgtacgact ggaaagccgg gaagaagggc ggataggagg gagaaaggga gaaggggaga
      961 agcggaaccc ttcccccttc cccttttccc ctcttctccc gccccgctat cttcgtgggt
     1021 tccccctgac aaacccggcg ccgaggcacg atggagtctt ctgccgcggg gcaggtcgag
     1081 acgatccccc gcactcactc gcagcggatc ggtcggcctt cccttcctcc ccaacgcacg
     1141 ctgctcacat ggctgttcgt cggccgtctg gtcctggcgc tcgcgacgct ggtgggagcg
     1201 tcgctggtgt ggacccagcg tcccgacgtt tcgttcatca gcaccattac cgtggtcttc
     1261 gcgctcacct ttaccggcta tggcgcctgg gccgtattcg tgcggggcgt catcccgtcc
     1321 agagcgttcc ttcagatcca ggccctggtc gacctcggcc tggtcaccgc gctggttcac
     1381 ctggcaggcg aacccaagtc ggccttcccg gcgctctacg tgctcgtggt cgcggcgtac
     1441 acgatgctgc tgccgctggg cgcaggggtc ttcactgcgg tggcagcctc cgcgatgtat
     1501 ctgggcgaca tcctgctcgg ccagaccgcg cgcccagaca ccgccctctg gatccagttc
     1561 ggggtgttca acgtcgtctt cctgctcgtc gcagtgctga gtcagcgtct gcgcgaagcg
     1621 ggcgtcgagc aggagtcact cgagaccgag ctcgcgcggg tgaggttgga agcggacgac
     1681 attctgcgca atatccgatc gggcgtgctc acggttgacg gccgcggccg actggcattc
     1741 atcaatccga ccgccgaacg aatgctgcgg ctgcaggcta gctcactgat cggccagccc
     1801 gtgctggagc acctcaaggc ccgctccagc gagttatggg ccgccatcaa cagcggaatc
     1861 cgcgccgggc gaaagatcag tcgaggcgag ggcctggttg taagcgcaca ggggcgtcaa
     1921 ttccccatcg gcctgagcac caccgtcttc cagcaggaaa gccaggaaac accctcggta
     1981 acggcgattt tcaccgatat ttccgacctc aagcagcttc aggagctgca cctccgggcc
     2041 gagcggttgg aggcggtggc tgcgctgagt gcctcgctgg ctcatgaaat tcgaaatccg
     2101 ctcgcctcga tccgaagctc ggtcgagcaa ctctcgcgcg cgaagtacgc gaccagcgac
     2161 gagcgattcc ttgccgatct catcgtccgc gagagcgacc ggctgagccg gttgctgaac
     2221 gaattcctgg acttctcccg tgtgcgtgcc accgagttcg tcccggtgga tctgcgcgac
     2281 gtggcgctgg cggtgactcg cctggtgaaa gagcatcccg actgcccggc ggaggccgtt
     2341 ctggaggtgc atggcgcccg cactgtcctc gacggcgacg ccgacctgct tcaccgggtg
     2401 atcgcaaacc tggtactcaa cgccgtgcag gcagccaagg gaagcaaagt gtgcgtgacc
     2461 gtatcggtgg gcgaagcaga cggtggcgcc gaccttccgc acgggcattt ggatcgtgcc
     2521 gttcggctgg aaatccggga tgacggtccg ggaattccgg acgagattag ggagcgtgtc
     2581 ttcgagccgt tcgtatcggg ccggcccggc ggtagcggtc ttgggctgtc cattgtacaa
     2641 cgggcggtag aggcgcatcg agggctggtg ttggtagact ccaaaccggg agccggtacc
     2701 gctttcacgg tgctactgcc ggtcgagtgg acgcctgagg agggagcatg agtcagaagc
     2761 cttcggtgct cgtggttgac gacgagtccg gaatcctcga tacgctccgc atcctgctgc
     2821 ggaacgaggg attcgaggtc accacggcac aaggcggcaa ggccgggctg gagcagattc
     2881 ggagcggcag tcacgacatc atcctgtcgg acgttcggat gccccaggtc tcggggctcg
     2941 acattctgca ggcggcgcgg gagcaggatc cgatgacgcc ggtcatcctg atgacggcgc
     3001 aggcatcgct tcagagcgct atcgccgccg tcaactcggg tgcgttccac tacatccaga
     3061 aaccgttctc gaacgacgag ctggtcgcaa tcctgcgccg ggcctgcgag ttccgcgcgg
     3121 tgcgggtgga aaacaagcag ctcaagcagg agattaggcg gcgcgagcgc gcaggcacca
     3181 ccgtcactcg tccgatcggg aagtcgaagc gattcgtcga agtcctgaag ctggcggaac
     3241 acgtcgcacc caccgactcg acggttctca tccagggaga gagcgggacc ggcaaggagg
     3301 tggtggcgcg ctacatccac aacctctcca gccggagcga cgggccgttc ctctcgatca
     3361 actgtggcgc cctgcccgaa aacctgctcg agagcgaatt gttcggtcac gtgaagggct
     3421 cgttcaccgg cgcagtgcgg gacaagcagg gtctgttcgc ggctgcgcgc ggcgggagct
     3481 tctttcttga cgaagtgggc gagatgccac cgtcgctcca ggtaaagctg ctccgcgtga
     3541 tccaggagcg ggaagtcatc ccggtgggcg caaccgaagc aatcccggta gacgttcgga
     3601 tcatcgccgc cacgaaccgc gatctcgaag aagaggtccg gcgggggaat ttcaggtcag
     3661 acttgttcta tcgcctcaac gtgatcgccg taaacctgcc ggcactacgt gagcggcgcg
     3721 acgacttgat ctcgctgatc gagctgttcc tcgagaatct ggggcaggag accggcggcg
     3781 aagcgaaggc gctctcgcag gacgccctgg atgcggttac ggtgtacgaa tggccgggga
     3841 atgtgcgtga gctggagaat gcgctggagc atgcggtagt gctcagcaag ggcagtctca
     3901 tcgaggccgg tgcgctgccc gagcgcatca ccaagcggaa gaaggaaccg ctggtagcgg
     3961 agcgctctta caagaatccc acactcgagg tcatcgagcg cgcctacatc atgtacgtcc
     4021 ttcaagccga aggcggcaac aaaacccgcg cggccgaagt gctgggcatc gatccatcca
     4081 cgctctaccg gaaactgtcg cggtacgagg gcgaggccgt ctgacggcta agtctccgcg
     4141 cgactggctg ggaatcgcct tcgttgctat cacggcctgc gcagtctatg cgcagaccgc
     4201 gcgatatgac tttgcctacg acgacctgca ggtcatccgc gaacggccgc tcttccactc
     4261 attggccaac tggcgcgaga tcctgacggc gagctggtgg cccaatcaca acctctaccg
     4321 ccctctcacc gcgctcaccc tggcgatgaa ctgggcggcg agcggcggaa accccaccgc
     4381 gtttcatgtc accaacatcg ccctgcacgc ggccgctgcg gtgctggcct atttgctggc
     4441 cactcgtctg ctgggcgtcg gtggtggagc tctgaccgcc ggattgctgt tcgcggtgca
     4501 tccggtgcat gtggaagcga tcgccaatct ggtgggtcga gccgaggtgc tcgccaccat
     4561 cttcgtcatc gtcgctgtat tgctttacgg tctcgatgga cggctggctg aatctggcga
     4621 cgatcggtcc tggaaacgat atgcgaccgc cgcgggcacg ctggtagcga ccgcgttcgc
     4681 actggcctcc aaggaatccg ccttcgccct tccgggcttg ttgttgctgc tggactggct
     4741 ggacgcgtcg ttgcgcggga ggcgggggct gggcgtggag cggcactggg tcctatggct
     4801 cggcgcggtg gccgtcgcgt ttgcctggct gctctggcgc gccaatgtgg tcggcgacct
     4861 caccggcaag gaggccgcac ctggggtcga ggacacgggg ctggtcgggc ggacggtggc
     4921 gatgctgccg gtcgtgcttc aatatggccg cctcctgttt ttcccggccc gtctctccgc
     4981 cgactactca cccaactttc tcgtgctggg ttcctggctt acgctccgca ctctggcggg
     5041 gctcctcgcg ctgatcgcgt gcggggccgt tgcctgggtg gcatggcgac gtgcgccggt
     5101 tgtgacgttc tcgctgggtt ggataggtgc gaccatcctg gtggtcgcga acatcctggt
     5161 cccgaccggc gtgatcctgg ccgagcgtac gctctatctc ccgtcggtcg gggccgtact
     5221 tctgctcggc tggattgcag gcgtggcctg gacacgtcgg cctcttgcca cggccacggt
     5281 cgtcggcctt ctcgtcgcgg cgggcggcgc tcgcacaatg acccgcaatc cggtatggcg
     5341 aaacaatgcg accctgttcc ctcagatcgt gcgcgatgcg ccgggctcgt cgcacgcggc
     5401 gtgggtggcc ggtggaatgg cggaggaggc cggcaatctg cccgaggcgg agcggatgta
     5461 ccgccagtcg ctcgagatct atccgctggc ctggaatgcc tggcgagacc ttggtcgggt
     5521 gatcaatgcg cagggtcggt atgccgaagc cggtgatgca tggtg
//
LOCUS       DASRJQ010000304         4109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_35386, whole genome shotgun sequence.
ACCESSION   DASRJQ010000304 DASRJQ010000000
VERSION     DASRJQ010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35386"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1962)
                     /locus_tag="VFB89_06795"
     CDS             complement(<1..1962)
                     /locus_tag="VFB89_06795"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZM27046.1"
                     /translation="MRRIAALVLRIAVLVLLVSLVEVSVAAQMALAQGDTQRPRVSSS
                     SPSAGAIGVSITGNVTATFNEPVQPASIAFVLRDASNAIVPSSLSYSAATRTVTLDPQ
                     SVLQPGKTYIATLSAAADLAGNTLSPSVVWSFTTHPGFQEAIVFAGLVEPTSIQFAPD
                     GRVFVAEKSGLIKVFANLLSTTPTVFADLRAKVYSYGGSGLLGMALDPGFPALPYVYV
                     LYTYDAPIGGTAPVWGDGCPPANADQCMVSGRLSRLQASGNVMIGAEQVLVENWFQRY
                     PDQPVGGLAFGPDGALYASAGDGASSAFVDLGQGFSPSPDPPSEGGALRSQDLRTPAD
                     PVALSGTVIRVHPDTGAPVRQTTSMLAGTPTVDAHGVKSYPVTSVFQGPQPTIVRVLE
                     PTSPAPGKLHRHLYVLPVETGVTSQSSTYSDGLEELRLLDVHNRYNLTLIAPSFIIEP
                     WYGDHQTDVSRWLESFIVRDLVPFGDSFAAATEIPQRWALGFSKSGNGALTLILRNPN
                     VFSAAAAWDAPAQFTDISQFQMAQNFGTNQNFDRYEIPALVLSSAQAFSTQNRLWVSG
                     DVSAWTSHMAQLDTQMSQAGVLHTFVNNGTRAHHWASGWLEGAVASLDAHATPVAPVD
                     PNAQRIVAYGLRNPSRIAFRPGTDELWIAD"
     gene            2365..3687
                     /locus_tag="VFB89_06800"
     CDS             2365..3687
                     /locus_tag="VFB89_06800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HZM27047.1"
                     /translation="MSDVDLLKSRIARREYTVGVVGLGYVGLPLVLRFGDVGFRVIGF
                     DVDAAKVKQLNDGGSYIQHVPAARVEALRAAGRFEATTALERLAEPDAIIICVPTPLT
                     RHRDPDLQYVEKTADAVAAVLRRNQLVCLESTTYPGTTDEIVLPRLQAGGLRVGEDFF
                     LAFSPEREDPGNSQFDTATIPKVVGGVTPACLALAAALYAEAIKQVVPVGSTQVAEAT
                     KILENVYRAVNIALVNELKIAFERMGINVWDVIEAAKTKPFGFQPFYPGPGLGGHCIP
                     IDPFYLTWKAREYGVATRFIELAGEINAGMPAYVILKLMEALNERGKPLKGSRILVLG
                     AAYKKDTDDSRESPGLEIMEELFHKGAKVDYSDPHLPRLPIVRKHKIDLASVVLSEAS
                     IRPYDAVLLVTDHSRFPYELIHRSASLIVDSRNAFRARGLTGSHVVPA"
     gene            complement(3744..>4109)
                     /locus_tag="VFB89_06805"
     CDS             complement(3744..>4109)
                     /locus_tag="VFB89_06805"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HZM27048.1"
                     /translation="EVYNLYSNVDTNVPTVYAVRSAAPTQPLDARGTAAVPLNAWTHL
                     AATHDGTTLRLYVNGVQVGSRAVAGALLTSTGALRIGGNSIWGEFFQGRIDEVRVYNR
                     ALSPSEILTDMNTRVGSGQ"
BASE COUNT          740 a         1326 c         1351 g          692 t
ORIGIN      
        1 gtcggcgatc cacagctcgt ccgtgccagg acggaaggcg atccgggagg gattccgcaa
       61 cccgtaggcg acaatgcgct gggcgttcgg atccaccgga gccaccggcg tggcgtgcgc
      121 gtccagcgag gcgacagcgc cttcgagcca gccgctcgcc cagtggtgag cgcgggtccc
      181 attgttcacg aaggtgtgca ggacaccggc ctgagacatc tgcgtgtcga gctgagccat
      241 gtgggaggtc caggcgctga cgtccccgct aacccacagg cggttctggg tgctgaacgc
      301 ctgcgcgctg ctcagcacga gtgccggaat ttcgtaccga tcgaagttct ggttcgtccc
      361 gaagttctgc gccatctgaa actgcgaaat gtcggtgaac tgggcaggag cgtcccaggc
      421 cgccgccgcg ctgaagacgt tcggattgcg caggatgagg gtcagcgcgc cgttgccgga
      481 cttgctgaat ccgagcgccc accgctgtgg aatctcggtc gccgccgcga aactgtcccc
      541 gaatggcacc agatccctga caatgaaact ctccagccag cgtgagacgt cggtttggtg
      601 gtcgccgtac cacggctcga tgatgaagga gggcgcgatc agcgtgaggt tgtagcggtt
      661 gtggacgtcc aggaggcgca actcctcgag gccgtcactg taggtcgatg actggctggt
      721 gacgccggtt tcgaccggca ggacgtaaag gtggcggtgt agcttgccgg gcgccgggct
      781 ggtcggttcc agaacgcgaa cgatcgtggg ctgcgggccc tgaaagaccg acgtcactgg
      841 atagctcttc acgccgtggg cgtcgaccgt gggcgtcccg gcgagcatcg aggtcgtctg
      901 ccgtactggc gcgcccgtat ccgggtggac gcggatgacg gtgccgctga gggcgaccgg
      961 atccgcgggc gtccggagat cctggctgcg caaagcgccc ccttcgctgg gcgggtcggg
     1021 gctcgggctg aacccctggc cgagatccac gaacgcgctg ctcgctccgt ctccagcgct
     1081 ggcatagagg gctccgtcag gaccgaaggc caagcccccg acgggctgat ccgggtagcg
     1141 ctggaaccag ttctccacca ggacctgctc ggctccgatc atcacgttgc cgctcgcctg
     1201 cagccgcgac agccgcccgc tgaccataca ctggtcggca ttcgccggag ggcagccgtc
     1261 cccccagacc ggtgcggtcc cccctatggg tgcgtcatac gtatagagga catagacgta
     1321 cggcaacgcg gggaaaccag gatcgagggc catcccaagg agtccgcttc cgccgtagct
     1381 atagaccttg gcgcgcaggt cggcgaaaac cgtcggcgtc gtgctcaaca gattggcgaa
     1441 gaccttgatg aggccactct tctcggcgac gaagacccga ccatcgggag cgaactggat
     1501 ggatgtcggc tcgacaagcc cagcgaagac gatcgcctcc tggaagcccg gatgggtcgt
     1561 gaatgaccag accactgatg ggctcaacgt attgcccgct aaatcggccg cggcgctcag
     1621 cgtggcaatg tacgtcttgc cgggctgcag cacgctctgc ggatcgaggg tgacggtccg
     1681 ggtcgcggcg ctatagctca gcgaagatgg gacgatcgcg ttcgaggcgt cgcgaaggac
     1741 gaaggcgatc gaggctggct gcaccggttc gttgaaggtc gcggtgacgt tgcccgtgat
     1801 ggaaacacct atcgctcccg cggatgggga ggaactgctc acacgcgggc gttgggtgtc
     1861 gccctgcgcc agtgccatct gcgcagcgac cgacacttct acgaggctta ccaatagaac
     1921 cagcacagcg atccgaagaa ccagcgcggc gatccgacgc atcaagtccc gaactcctgg
     1981 cagaggacca agtcgaatag cgccacccaa tgtggcgcta ttgtgtcaac agagcggcct
     2041 catcacagaa gtctcaagag gcatgccgag actccagagt agtgcttttc aaggagtaag
     2101 gcggcggaaa gcgatgctca actgccgatc tgtaaaagat aatgacagcc cgtctgggtc
     2161 atcaagcgag acccgaaatc ccggtaaggg acgggcggca ttatagctcc gatcacaggg
     2221 agtgggccga gccagccgta caatcgtagc ctgtctctga attgattcgg ccggcccacc
     2281 aacgggggct cctgtgtgat cgcgatgaag gcactcaggt gtcgacccgc tatactgttt
     2341 tgagcttccg aaggaggcca gtcgatgtcg gatgtcgatc tactaaagag ccggatcgcc
     2401 cgccgggagt acaccgtcgg ggtcgtcggt ctcggctatg tgggcctgcc gctggttctg
     2461 cgcttcggtg atgtcggctt ccgggtcatc ggcttcgacg tggatgccgc caaggtcaaa
     2521 cagctcaacg acggcggcag ttacatccag cacgtcccgg cagcgcgcgt cgaggcactc
     2581 cgtgcggcgg gccgtttcga ggccacgact gctctcgagc ggctcgcaga gccggacgcc
     2641 atcatcatct gtgtgccgac ccccctgacg cggcatcgtg acccggatct acagtacgtc
     2701 gagaaaacgg ccgacgcggt ggcggcggtg ctgcgccgca accagctggt gtgcctggag
     2761 agcacaacat atccggggac gaccgacgag atcgtgctcc cgcggctgca ggcgggcggc
     2821 cttcgcgtgg gagaggactt cttcctcgcc ttctcgcccg agcgggaaga tcccggcaac
     2881 agccagttcg acacggccac catccccaag gtggtgggcg gcgtcacgcc ggcgtgcctg
     2941 gcgctggccg cggccctgta tgcggaggcc atcaagcagg tggtgccggt cggctctacg
     3001 caggtagccg aggccaccaa gatcctcgag aacgtctacc gggccgtgaa catcgccctg
     3061 gtcaacgagc tgaagatcgc cttcgagcgc atgggcatca acgtgtggga cgtcatcgag
     3121 gcagccaaga ccaagccctt cggcttccag cccttctatc cgggccccgg gctgggcggc
     3181 cactgcatcc cgattgaccc gttctatctg acgtggaagg cccgggagta cggcgtggcc
     3241 acacgcttca tcgagctcgc cggcgagatc aacgccggga tgcccgccta cgtcatcctg
     3301 aagctgatgg aggcgctcaa cgagcgcggc aagccgttga agggctcccg catcctggtg
     3361 ctcggggcgg cctacaagaa ggacaccgac gactcacgcg aatcgccggg cctcgagatc
     3421 atggaggagc tcttccacaa gggcgccaag gtcgattaca gcgacccgca tctgccccgg
     3481 ctgccgatcg tgcgcaagca caaaatcgat ctcgcgtcgg tggtgctgtc ggaggcctcg
     3541 atacggccgt atgacgcggt gctgctggtc accgaccact cacgcttccc ctacgagctg
     3601 atccaccgga gcgcgtcgct gatcgtggac agccgcaacg ccttccgcgc gcgaggactt
     3661 accggctccc acgttgttcc cgcctgatcg cggggacgtc tcgacgagcc taaccccgag
     3721 cctcgatacc tgcctgcggg cagctactgc cccgagccga cgcgggtgtt catgtccgtc
     3781 aggatctctg acggcgacag cgcgcggttg tagactcgca cctcgtcgat gcggccctgg
     3841 aaaaactcgc cccagatact gttgcccccg atcctcagcg ccccggtcga agtcagcagg
     3901 gcgcccgcca ccgcccgact ccccacctgc acgccgttga cgtacaggcg cagcgtcgtt
     3961 ccatcgtgcg tcgccgccag gtgcgtccaa gcgttcagcg gcaccgccgc cgtcccccgc
     4021 gcgtccagcg gttgtgtggg cgccgcgctg cgcaccgcgt acaccgtcgg cacgttcgtg
     4081 tccacgttcg agtagaggtt gtagacctc
//
LOCUS       DASRJQ010000305         2138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2877404, whole genome shotgun sequence.
ACCESSION   DASRJQ010000305 DASRJQ010000000
VERSION     DASRJQ010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2877404"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VFB89_06810"
     CDS             complement(<1..193)
                     /locus_tag="VFB89_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27049.1"
                     /translation="MGYFRTAMLLAAMTALFMGIGYLVGGQTGMVVAFLVAAAMSLFA
                     YWNSDKMVLSMYGAREVDER"
     gene            418..870
                     /locus_tag="VFB89_06815"
     CDS             418..870
                     /locus_tag="VFB89_06815"
                     /inference="COORDINATES: protein motif:HMM:NF012272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin domain-containing protein"
                     /protein_id="HZM27050.1"
                     /translation="MSLTLLAPSPEPAISASPSLTREQKRQIRETFAVIEPASDLVAR
                     LFYMKSVDLDPSLGKLFNSPSRAQRRKFMAAMKVAVLSLDRLQSLQPILKLLGTRQRE
                     AGVKPSHYDAFQHAWVWTLEQSLQPHFPREAREAWSSLLGEMTRATAS"
     gene            complement(887..1294)
                     /locus_tag="VFB89_06820"
     CDS             complement(887..1294)
                     /locus_tag="VFB89_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27051.1"
                     /translation="MSWVKGLLIGLVVVAVLVFAIIAGLGMLGSGGGKEVRHISMGQR
                     TITVSHYKNLTQETTADGVRIMVDGHTIVATNDAITIDGTAQTFDPTQDVEITVDESG
                     AVQAKTLAADAPRPDEDEGIPEESGGDAAPPPQ"
     gene            complement(1348..>2138)
                     /locus_tag="VFB89_06825"
     CDS             complement(1348..>2138)
                     /locus_tag="VFB89_06825"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZM27052.1"
                     /translation="INPEAWEWYYRVVGEERCPIVDTWWQTETGGILITPLPGATKLK
                     PGSATLPFFGVQPAVVDGEGNLLEGAVSGNLVILDAWPAMARTIYGDHKRFADTYFST
                     YKGKYFTGDGCRRDADGYYWITGRVDDVINVAGHRMGTAEVESALVAHPKVSEAAVVG
                     YPHAVKGQGIYCYVTLMAGESGSDALKIELRDWVRKEIGPIASPDLIQFAPGLPKTRS
                     GKIMRRILRKIAEDDFSNLGDISTLADPAVVEDLVDKRQNRGTA"
BASE COUNT          378 a          705 c          655 g          400 t
ORIGIN      
        1 cgcgctcgtc gacctcgcgc gccccataca tggacagcac catcttgtcg gagttccaat
       61 acgcgaacag gctcatcgcc gccgcaacga gaaacgcgac gaccatgccg gtctggccgc
      121 cgaccagata accgatgccc atgaagaggg cggtcatcgc ggcaagcagc atggctgtgc
      181 ggaaataacc catcaaactc actcccagcg cgcgggggac gttggcccgc gctcacggtg
      241 aatatgttgg aagtgcctgc ccccatcaca agtatctggc gaccttggga aagccgcgcg
      301 ggcggcgctt tcgcagcggc aggaagaacg gccgttagtg atgcggttac cttaacgctt
      361 cctcaagggc tcgggctcat cctggcggga ggttcaatcc agctcagagt cgcgcccatg
      421 tccttgacac tgcttgctcc ttcgcccgag cccgcaatct ccgccagccc ctcgctcacc
      481 cgcgagcaga agcggcagat ccgcgaaacc ttcgccgtca tcgagcccgc ttccgatctc
      541 gtggcgcggc tgttctatat gaagtccgtc gatctcgacc cctcgctcgg caagctgttc
      601 aactctccgt cacgcgccca gcggcggaaa ttcatggcgg cgatgaaggt cgccgtgctg
      661 tcgctcgacc ggctccagtc gctccagcct atcctcaagc tgctcggcac gcgccagcga
      721 gaggcaggcg tcaagccaag ccattacgac gccttccagc acgcctgggt gtggacgctt
      781 gagcaatcgt tgcagcccca cttcccccgc gaggcgagag aagcctggtc gtcgctctta
      841 ggcgagatga cgcgcgccac cgcctcctaa aaaggccggc gcccatttat tgaggtggcg
      901 gcgcagcgtc gccgccactc tcctcgggaa ttccttcgtc ttcgtcgggc ctgggagcgt
      961 cggcggccaa ggtcttggcc tgcacggcgc cgctctcatc gacggtgatc tccacgtctt
     1021 gcgtaggatc gaaggtctgc gcggtgccgt cgatggtgat ggcatcgttg gtcgcgacga
     1081 tggtgtgacc gtcgaccatg atcctgacgc cgtccgccgt cgtctcctgg gtcagattct
     1141 tgtaatgcga cacggtgatg gtcctttggc ccattgagat gtgcctgacc tccttgcctc
     1201 caccggaacc gagcatgcca agccccgcga tgatggcgaa tacgagcacg gcaacgacga
     1261 cgagaccgat gagcagcccc ttgacccagg acatggcgat gtctttcccc tcagatctcg
     1321 cgggcctggc ggcccgcatt tcttgactca agcagttccg cggttctggc gcttgtcgac
     1381 gagatcctca accacggcag gatcggcaag tgtcgagatg tcgccgagat tggaaaaatc
     1441 gtcctcggcg atcttgcgca ggatgcggcg catgatcttg ccggagcggg tcttgggcag
     1501 cccaggcgcg aattggatca ggtccggcga ggcgatcggg ccgatctctt tgcgcaccca
     1561 gtcgcgcagc tcgatcttca gcgcatccga tccgctctct ccggccatca gcgtgacata
     1621 acaatagatg ccctggccct tgaccgcgtg cggatagccg acgaccgcgg cttccgacac
     1681 tttgggatgg gcgacgagag ccgactcgac ctcggccgtg cccatgcggt gcccggcgac
     1741 attgatcacg tcgtcaacgc ggccggtgat ccagtaatag ccgtcggcgt cgcggcggca
     1801 tccgtcgccg gtgaaatatt tgcccttgta ggtcgagaaa taggtgtcgg cgaagcgctt
     1861 gtggtcgccg tagatggtgc gcgccattgc cggccacgca tcgaggatca cgagattgcc
     1921 tgagaccgcc ccctccagca aattgccctc gccgtcgacc acggctggct ggacgccgaa
     1981 gaagggcagc gtcgccgagc cgggcttgag cttggtggcg ccaggcagag gcgtgatgag
     2041 gatgcctccg gtctcggtct gccaccaggt gtcgacgatt ggacaacgct cctcgccgac
     2101 cacgcggtaa taccactccc aggcttccgg gttgatcg
//
LOCUS       DASRJQ010000306         3267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1790574, whole genome shotgun sequence.
ACCESSION   DASRJQ010000306 DASRJQ010000000
VERSION     DASRJQ010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1790574"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VFB89_06830"
     CDS             complement(<1..822)
                     /locus_tag="VFB89_06830"
                     /inference="COORDINATES: protein motif:HMM:NF024807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HZM27053.1"
                     /translation="MQRLVLFLLCSLFGAQSVMVQSTAAVPMVSFGPNPVVAAGSSPH
                     SATTRALLGPLATSQEPPFPAQSHLTSDVRSRLAKAASDSTLEPWQRDFMLEVARHGA
                     EGTATQPSPALPSVGDEIAANDGSWIPIITPPAGRYAPAAIYDPVRDRMVVFGGVNDV
                     SNHNDVWALSLAGSPAWSELTPAGTPPSARYAHAAIYDPVRDRMVVFGGSDPHLRNDV
                     WALSLAGSPAWSELIPAGIPPAARDEHTAIYDPVRDRMVVFGGSGNRNDVWALSLA"
     gene            complement(1026..1102)
                     /locus_tag="VFB89_06835"
     tRNA            complement(1026..1102)
                     /locus_tag="VFB89_06835"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1066..1068),aa:Arg,seq:cct)
     gene            complement(1332..2072)
                     /locus_tag="VFB89_06840"
     CDS             complement(1332..2072)
                     /locus_tag="VFB89_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZM27054.1"
                     /translation="MLPAVHLVCHRTPLIILAGDVGTGKTTLAETFADALARSEKMAI
                     RVMRLSLRTRGHGGTGEATDRIGKAFDVVREETRIHQAVILVIDEADALAQSRALKAM
                     HHEDRAGVNALIRGLDSLVAAAARVLAVMCTNRLGAIDPAVRRRAAGLFILERPTEAQ
                     RHSLLANALGDALKEDEIAEIARLTGPRNASGVGYTWSDLSERLIPSAVLAAYPHSAL
                     SYELLREQLTLTAPTKPFTSKDESPTRS"
     gene            2079..2333
                     /locus_tag="VFB89_06845"
     CDS             2079..2333
                     /locus_tag="VFB89_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27055.1"
                     /translation="MELLAPLLERAWIHQHGGLLEKLSQNVIATDQGAKAALGLGVRK
                     VKNLIKQAVKRVHGKNLDPGQRAIARVSASMPGPGPGGRV"
BASE COUNT          526 a         1066 c         1092 g          583 t
ORIGIN      
        1 cgcgagcgag agcgcccaca cgtcgttgcg gttgccgctt cccccgaaca ccaccatgcg
       61 gtcgcgcacc gggtcgtaga tcgccgtgtg ctcgtcgcgc gcggccggcg gaatccccgc
      121 gggtatgagc tcgctccagg ccgggctgcc cgcgagcgag agcgcccaca cgtcgttgcg
      181 taagtgcggg tctgatcccc cgaacaccac catgcggtcg cgcaccgggt cgtagatggc
      241 cgcgtgcgcg tagcgcgcgg aaggcggggt cccggcgggg gtgagctcgc tccaggctgg
      301 gctgcccgca agcgagagcg cccacacgtc gttgtggttg ctgacgtcat taactccccc
      361 gaacaccacc atgcggtcgc gcaccgggtc gtagatggcc gcgggcgcgt agcgcccggc
      421 cggcggggta ataatcggga tccaagatcc gtcattggcg gcgatctcgt cgccgacgct
      481 agggagtgcc ggagatggct gggtcgcggt gccctccgct ccgtggcgtg ccacctccag
      541 catgaagtca cgctgccagg gctccagcgt cgaatcgctc gccgccttgg ctagtcggct
      601 gcgcacgtcg gaggtgagat gggactgggc ggggaacggc ggctcctggc tagtcgcgag
      661 ggggcccagc aaagcgcggg tagtcgccga gtgaggactg ctcccggccg cgacgacggg
      721 gttcggcccg aagctcacca ttgggacagc cgcggtagac tgaaccatga cgctctgcgc
      781 gccgaacaac gaacacagca gaaacagaac caaccgctgc atggcgtctc cggcgtcaat
      841 ggagtgcggc cccggacccg cctgcgctga ggagctgctt gcgccgtctc ctagctcaaa
      901 taaacgctac tccacatgtc ccatgtgcgc gaagccattt ctgctggcct gcaaagcctt
      961 ggcccccgcc ggtgctaacc ggcaggggca gagcgtattg ccttgcgtcc caagtcgaaa
     1021 gctggtggtg gccccggcag gagtcgaacc tgcggcacgc ggtttaggaa accgctgctc
     1081 tatccgtctg agctacgggg ccacgagccg ggcactgtgg ccgatgactt cgccttccgc
     1141 aagcgcgctc gcgtggtgct ggagctcacg cgaagttgct tggctacggt tgcgcaccct
     1201 tccgacgtct cggtccactc ttgtcgcgct cgtcctcctc atcgttggca ctcacgatgc
     1261 cgccgaggat gccgggagga agcgggcgta ctggccggcg cgcggagcct gtggctcgcg
     1321 cgtcagttac ttcatgagcg cgttggcgac tcgtccttgc tggtgaacgg tttggtgggg
     1381 gccgtgagcg tcagctgctc gcgtaggagc tcgtagctga gcgcgctgtg gggataggcg
     1441 gccagtacag ccgaggggat gagtctctcc gataggtcgg accacgtgta accgacgccg
     1501 gacgcgttac ggggtccggt cagacgcgcg atttcggcga tctcgtcctc cttcaacgcg
     1561 tccccgaggg cgtttgccag cagcgaatgg cgctgtgcct cggtggggcg ctccaggatg
     1621 aagaggccag ccgcgcggcg acgaactgcc gggtcgatcg cacccagccg gttcgtgcac
     1681 atcaccgcga ggacccgggc cgccgccgcg acgagagagt cgaggccgcg gatcagcgcg
     1741 ttcactccgg cacggtcctc gtgatgcatg gccttgagcg cgcgggactg ggccagcgcg
     1801 tcggcttcgt cgatgaccag gatcaccgct tgatggatac gcgtttcctc gcgcacgaca
     1861 tcaaaggcct tgccgatcct gtccgtcgct tccccggtcc cgccgtgccc gcgcgtgcgc
     1921 agcgacaggc gcatcacccg aatcgccatc ttttcgcttc gcgccagcgc gtccgcgaac
     1981 gtctctgcca gggtcgtctt gccggtcccg acgtctccag ccaagatgat gaggggcgta
     2041 cggtggcaga cgagatgtac cgcgggcagc acacgtccgt ggaattgctc gctccactgc
     2101 tcgagagggc gtggattcac cagcacggtg gcctgcttga gaagctgagc cagaatgtca
     2161 tcgcaaccga tcagggcgcc aaggcggcgc tgggcctggg cgttcggaag gtcaagaacc
     2221 tgatcaaaca ggccgtcaag cgggttcatg gcaagaacct cgatcctggt cagcgtgcca
     2281 tcgcgcgagt cagcgcctcg atgccggggc caggcccggg cggtcgggtc tagagagtct
     2341 cgtttgagcg ctgctcgggt cgcgcggcag gggtcgagaa gacacctgct ccgtcgcacc
     2401 gcgacgtccc gctccctaca agagatcggc cgtcgtcatc ggggtcttga gcacgcatgg
     2461 tcgggcacgt ggcccaaatg gttcgcctga caacctggca ctgagcgtcc ctcctcagtc
     2521 agtcgctgct tgactggctg gctggcgtcc taagcgagcc agctcgatct gctcaccgcc
     2581 aaccgctcga ggtgccagag tggctacgac tttggctacg agaaccgtgt caatgcatgt
     2641 ccgctcgtgt ccgcttctgt ccggtgaacc gcgaagccct cgcgctttca gctcgcgcga
     2701 gggcttcagg atcagcgctg tccgcaatgg cttacgtgaa cgtctcgttt tcgcgcccca
     2761 gcggccacca ttcgctcagg aaaccgctgc tctatccgtc tgagctactg ggccacgggc
     2821 cagcaactta gcatagcccg cgatctgcga agcagcggtc tggtgaaccg ctgctcgccc
     2881 gcttcgcggc gcgggtggca aggcacgcac gcacctgcgt gaatcgactt caatctcgcg
     2941 cgttggctca agacgaagat gacgccttcg tgaagggccc atgttcggga gccgatgact
     3001 cagctatggc gcggttggtt cgaatccatt tggtgcgcct ccacgtacca cggctatcag
     3061 atcctcgatc ggcaaggtcg ccttctccac aagccggcta gcgtgagcat gccccgctgg
     3121 tctcccgatg gtcgctggcc tgcggtgagc cgatggtctc gcgagcttcc ctaccagctt
     3181 tcagactgac ttgggagacg atgcaacgca gtcggatttc gggacaatgc tggagttgcc
     3241 cgcgctggag aagtcacctc gttgagg
//
LOCUS       DASRJQ010000307         5540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1445426, whole genome shotgun sequence.
ACCESSION   DASRJQ010000307 DASRJQ010000000
VERSION     DASRJQ010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5540
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445426"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(346..1104)
                     /locus_tag="VFB89_06850"
     CDS             complement(346..1104)
                     /locus_tag="VFB89_06850"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZM27056.1"
                     /translation="MTATSTLAPARHKQELLGQTVVVIGGSLGIGLETARVAREEGAD
                     VIITARDPDRVRRVGQDLGANTAAFDATDFERLGKFFDQLPTPIDHVLVTGPGPYYAP
                     LKELDFDKARRDVEAHLLLPLQVARNAIGKVRPGGTLLFMGGTGGRRTAPGLALISAL
                     TAAGPAMTKNLALELAPIRVNLIAAGFVDTPLSAAILGGELDSRREQLRTTLPVRRVI
                     GPADIAALAVHLMTNTAVTGATFDIDGGQQLVEG"
     gene            complement(1119..1712)
                     /locus_tag="VFB89_06855"
     CDS             complement(1119..1712)
                     /locus_tag="VFB89_06855"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HZM27057.1"
                     /translation="MRPDVAPGRTFPDYELPDHTSMPRKLSELQGDDPLILTLARGHY
                     CPKEHQQHLELAAFYPKIAVAYTQIATISTDDHHTLQEFRASVGAQWTFLSDPGRMVQ
                     KDLDIQEYTDPEHNPMIPHTLVLKPGLVIHRIYNGYWFWGRPSVVDLWHDLRAVTSEI
                     RPDWDLSTPGLRKRWDAGDLSPFHGWNKQAGQKPASV"
     gene            complement(1746..2408)
                     /locus_tag="VFB89_06860"
     CDS             complement(1746..2408)
                     /locus_tag="VFB89_06860"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZM27058.1"
                     /translation="MSSTAIRSKDATSDARVGKLDLKLEVVTIPVSDVDRAKEFYQNL
                     GWRLDADFSAGGSRVVQFTPPGSECSIHFGTNLTPVAPGSAQSLWLIVSDIDGARDEL
                     VKRGVEVSEVFHFASFNRVDPAGRLSGPDPDRRTYASFASFSDPDGNGWLLQEITTRL
                     PGRVAGDTTYASVGDLSQALRRAEAAHGQHEKRTGQRDADWPSWYAEYMIAEQAGKEL
                     PT"
     gene            3066..4064
                     /locus_tag="VFB89_06865"
     CDS             3066..4064
                     /locus_tag="VFB89_06865"
                     /inference="COORDINATES: protein motif:HMM:NF012425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family peroxidase"
                     /protein_id="HZM27059.1"
                     /translation="MSSHRSRIGVFLCLAGFPIGMAAQQRPATPSLPQQIADAMVQLN
                     GGIHTGYRFTHAKGIVTTGTFTPAPGAKAISTAAHLKGGPVPVIVRFSDGTGVPQISD
                     TASAASPRGMAVRFTLPGGAFTDIVANSHNGFFVGTGEDFLAMLKAIAATTPTSPHPS
                     PIEAFLGSHPRALKVIQDSKPLPKSFATLDFYGNNAFIFVDSAGTRRAGRYQIIPLAG
                     VAHLVAAAAGKVGPNYLFDDLPKRLAKGPVKFRLFVQLANKGDPTNDGSVVWPADRKR
                     VELGTISIEKVAPDNEALQRSLAFNPIYLTKGIELSDDPFPALRSAVYALSVAHRR"
     gene            4324..5052
                     /locus_tag="VFB89_06870"
     CDS             4324..5052
                     /locus_tag="VFB89_06870"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZM27060.1"
                     /translation="MPTQSTLTQKPRVVSHDQWVAERVAFMAKEKELTRLRDELSQKR
                     RELPWEKVTKPYVFDTPGGKQTLAELFDGRSQLVVYHLMFAPSWEEACKSCSFWADNF
                     NGIDIHLAHRDITFLAISRAPLPKLEAYKKRMGWTFKWVSTGESSFNYDFQVSHNPEA
                     AKDGTAYYNYGTGIKVGEEMPGVSVFYKDQKGDIYHTYSAYSRGIDLLNGAYNYVDLT
                     PKGRDEEGLKDPMAWLRRHDQYED"
     gene            5056..>5540
                     /locus_tag="VFB89_06875"
     CDS             5056..>5540
                     /locus_tag="VFB89_06875"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HZM27061.1"
                     /translation="MTGMGMSQQTWYGAAAGFMGMWMAMMVPMMLPSLIPMLSRYRRS
                     VRGGDGIHLHGLTALAGVGYYVIWAIVGAAVYAVGAGLMAVEMRWGVVERWLPVVTGV
                     ALLAAGGMQFTSWKARQLAICRDGSVCDSASVPNARGAWGHGLTLGVRCSLCCGSLML
                     AL"
BASE COUNT          952 a         1767 c         1810 g         1011 t
ORIGIN      
        1 ttccgcgccc gccgccggct ccgagaggca ctgtacgacg aagcggtcga tatgggctat
       61 gtcgggcgcg gcctccagac gtgacggcgt ctggatggcg acgggcgagg ccacgagcac
      121 gcccatcttc gagagaacct cgcaacagcc gagcccgatc tcccggatga gccgctaaag
      181 gagttggacg aggtggcccc tctcgtcagg attgtgcaca cggcttgaaa tggcaggggt
      241 caccgtcggc agtggcggcc ctttccttta tatatcgcag aacccgccat gccgtcgggg
      301 tccgcgacta ggcgcttaag tccgatcaat cggagcgcct ttgccttacc cctccaccag
      361 ctgctggcct ccgtcaatgt cgaacgtcgc gccggtcacc gccgtgttcg tcatgagatg
      421 cacggccagc gccgcgatgt cggcgggtcc gatcacccgc cggacgggaa gcgtcgtccg
      481 gagctgctcg cgccgcgagt cgagctcgcc gcccaggatc gcagctgaca gcggcgtgtc
      541 gacgaacccg gccgcgatca ggtttacccg gatgggtgcg agctcgagcg caaggttctt
      601 cgtcatcgcg ggacccgcgg ccgttagcgc cgatatgagc gcgaggcccg gcgccgtgcg
      661 gcggccgccg gtgccgccca tgaagagtag ggtcccgccc gggcgaacct tcccgatggc
      721 gttccgggcc acctgcagcg gcagcaggag atgggcttcg acgtcgcggc gcgccttgtc
      781 gaagtcgagc tccttcagcg gcgcgtagta ggggccgggg cccgtgacca gcacgtggtc
      841 gatcggcgtg ggcaactggt cgaaaaattt ccccagccgt tcgaagtcag tcgcgtcgaa
      901 agccgcagtg ttggcgccga ggtcctggcc gacacggcgc acgcgatctg gatcgcgcgc
      961 tgtgatgatc acgtcggctc cctcctcgcg cgcgacccgg gcggtctcga gccctatgcc
     1021 caagctcccg ccgatcacaa ccacggtttg cccgagcagc tcctgcttgt ggcgggccgg
     1081 agcgagtgtg gatgtggcgg tcatcattcc tccttccgtc aaaccgaggc cggtttctga
     1141 ccagcctgct tgttccatcc gtgaaagggt gacaagtcgc ccgcgtccca gcgcttgcgg
     1201 aggccaggag tgctcaggtc ccagtcgggg cggatctcgc tcgtcacggc acgcaggtcg
     1261 tgccagagat cgacgaccga ggggcggccc cagaaccagt agccgttgta gatgcggtgt
     1321 atcacgagcc ccggcttgag cacgagcgtg tgcgggatca tcgggttgtg ctcggggtcg
     1381 gtgtactcct gaatgtcgag gtccttctga accattcggc cgggatcgga gaggaaggtc
     1441 cactgggcgc ccacggacgc ccggaactcc tggagcgtat ggtggtcgtc ggtcgagatc
     1501 gtggcgatct gggtgtaggc cacggcgatc ttggggtaga acgcggcgag ctccagatgc
     1561 tgctggtgct ccttcgggca gtagtggccg cgcgcgagcg tgaggatcag cgggtcgtcg
     1621 ccctggagct cgctgagctt gcgtggcatg ctcgtgtgat cgggcagctc gtagtcgggg
     1681 aaggtcctcc cgggcgcgac gtcaggccgc atggttggac cccttcccga tcacgtcgtg
     1741 atcgctcacg tcggcagctc tttgccggcc tgctccgcga tcatgtactc ggcgtaccag
     1801 ctgggccagt ctgcgtcccg ctgccccgtc cgcttctcgt gctggccgtg cgcggcctca
     1861 gcgcgccgca gcgcctgcga caggtcgccc acggacgcgt aggtggtgtc gccggcgacc
     1921 cgtccgggca gccgtgtcgt gatctcctgc aggagccagc cgttgccgtc cggatcgctg
     1981 aacgacgcga acgatgcgta ggtgcgccgg tccggatctg ggccgctcaa tcgtccggca
     2041 gggtcgactc ggttgaagct ggcgaagtga aacacctcgc tcacctcgac accgcgcttc
     2101 accagctcgt cacgggcacc gtcgatatcg gacacgatca gccacaagct ctgggcggac
     2161 ccgggggcga caggcgtgag attcgtgccg aagtgaatcg agcactcgga gccgggaggg
     2221 gtaaactgga ctactcggct gccgcccgcg ctgaagtcgg cgtcgagccg ccacccgaga
     2281 ttctgataga actccttcgc gcggtcgaca tcggagaccg ggatggtgac cacctcgagc
     2341 ttcaggtcga gcttcccgac ccgcgcgtcg ctggtcgcgt ccttactacg gattgccgtg
     2401 ctgctcatgg ctgcctcctg agttgtatcg gtatcgggac gacggtcggg cgcccgccgc
     2461 taagatcgtc acagccgcag ggatcgagat gtgaagcgag gggcatgcag ggacaaacag
     2521 gcatcataag ttgatcagct ttctgagtct tgaactccct gctctcgtca tccagggaaa
     2581 gcggcccgaa tggtcggcgg gagcttgcgc aagtgtatgc ttggctgaag cggcacctac
     2641 tgctgccgag gcctgcccaa ttcggctcag ggtcgccctc gaacagcgtc acacaattgt
     2701 tacatgcccc agcttattcg ttacgcacta caacgcgtag tttggccgag cttgggacga
     2761 gacttcgccc tgatctgaaa cggctggtgg aggcacggta tggtcgcact cgagcgtaag
     2821 ccacgacatg gatctgtcat tcgagagctc gccaccaatc tcgaccgcgt aatggcgggt
     2881 caacccgagc acgtctgcct tgcgccgcgg gagggagagc catcctgcgc gtgctacctg
     2941 gacgcacgcc atgaaggctg gcatcgctgc ttgtgtggcg ctgagtggcc ggctgacacg
     3001 gtaatcgcca tcaagagggt gtgagccccc ggcccactcc catccattct gtcggaggtt
     3061 tgcgcgtgtc atcccaccgg tcccgcatcg gcgttttcct ctgtctcgcc ggcttcccca
     3121 tcggcatggc cgcgcagcag cgtccggcaa cgccgagtct gccacagcag atcgccgatg
     3181 ccatggtaca gctcaatggc ggcatccata ccgggtatcg cttcactcat gccaaaggaa
     3241 tcgtgacgac cgggacgttc acgcccgcac caggagccaa ggcgatcagc accgcagccc
     3301 accttaaagg cggaccggtt ccggttatcg tgcggttctc cgacgggacg ggtgtgcccc
     3361 agatttcgga taccgcctct gccgcctcgc ctcggggcat ggcggtccgg ttcacgctgc
     3421 ccggcggtgc ctttaccgac atcgtcgcga actcgcacaa cgggttcttc gtcggcactg
     3481 gcgaagactt cctggcaatg ctcaaagcga tcgctgccac gacgcctacc agcccacacc
     3541 cgtcgccgat cgaagccttc ctgggcagtc atccacgcgc gctcaaggtc atccaggatt
     3601 ccaagccact cccgaagagc tttgcgacac tcgatttcta cgggaacaac gcgttcatct
     3661 tcgtggacag cgcggggacc cgccgggcgg gccggtacca gattattcct ctcgcgggag
     3721 tggctcacct ggttgctgca gcggctggca aggtggggcc caactacctg ttcgacgacc
     3781 tgccgaagcg cctggccaag ggaccggtca aattccgttt gttcgtgcag ctggcgaata
     3841 agggcgaccc gaccaacgat ggctccgtgg tatggcccgc cgatcggaaa cgggttgagc
     3901 tcggtaccat cagtatcgag aaagtcgcgc ccgacaacga ggcgctccag cgatcgctcg
     3961 ctttcaaccc gatctacctc accaagggaa tcgagctctc cgacgatccc ttccctgcac
     4021 tccgatcggc ggtgtacgcg ttgtcagtcg cgcatcgtcg gtagcgcttc tcgaacgctc
     4081 gttttccagg caccgctagg taccgctgca gtaccagggc ggtgccgctt catcggtcac
     4141 agccatcgga tcgcccgctg ggtcggatcg gcaatggcgc catacacgtg gtccaacagg
     4201 ggctccagca caactgaccg gggattgcat tcaacctcat ggttgaatat cattattcaa
     4261 ccgagcggtt gactgttggg gaatgagcgc ggttgcgcca ccctggaccc caaggaggac
     4321 gttatgccaa cccagagcac cctcacccag aaaccccgcg tggtctctca cgaccagtgg
     4381 gtcgccgagc gagttgcgtt catggccaag gaaaaggagc taacccggct tcgggacgag
     4441 ctgagtcaaa agcgtcgcga gctgccgtgg gaaaaggtca ccaagccgta cgtcttcgac
     4501 accccgggcg gtaagcagac gctggccgag ctgttcgacg gtcgcagcca gctggtcgtc
     4561 taccacctca tgttcgcccc ctcatgggag gaggcgtgca agagctgctc gttctgggcc
     4621 gacaacttca acggaatcga catccacctg gcgcatcgcg acatcacctt cctcgcaatc
     4681 tcgcgggcgc ctctcccgaa gctcgaggcg tacaagaagc ggatgggctg gaccttcaag
     4741 tgggtctcca ccggcgagag cagcttcaac tacgacttcc aggtgtcgca caaccctgag
     4801 gcggccaagg acggcacggc ctactacaac tatggcacgg gcatcaaagt cggcgaggaa
     4861 atgcccggcg tcagcgtgtt ctacaaggat cagaagggcg acatttatca cacctactcg
     4921 gcctactcgc gcgggattga tttgctgaac ggcgcgtaca actacgtcga tctgacgccc
     4981 aaaggacgcg acgaagaggg gctcaaggac ccgatggcgt ggctacggcg ccacgaccag
     5041 tacgaggact gacagatgac cgggatgggc atgtcccagc agacctggta cggcgccgcg
     5101 gccgggttca tggggatgtg gatggcgatg atggtgccga tgatgctgcc atccctgatc
     5161 ccgatgttgt cgcggtatcg tcggtccgtg cgcggcgggg atggcattca tctgcacgga
     5221 ctgaccgcgc tggccggcgt gggctactac gtcatctggg ccattgtcgg tgcggcggtc
     5281 tacgccgtcg gcgctggtct gatggcggtt gagatgcgct ggggggtcgt ggagcgatgg
     5341 ctgccggtcg tgaccggcgt ggcgcttctc gcagcgggcg gcatgcagtt cacttcgtgg
     5401 aaggcgcggc aactggcgat ctgccgcgac ggatcagtgt gtgacagcgc gtccgtgcct
     5461 aacgcccgag gcgcgtgggg ccatggcctc acgttgggcg tgcgctgcag cctctgctgc
     5521 gggagtctga tgctggcgct
//
LOCUS       DASRJQ010000308         3002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2796867, whole genome shotgun sequence.
ACCESSION   DASRJQ010000308 DASRJQ010000000
VERSION     DASRJQ010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2796867"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1522)
                     /locus_tag="VFB89_06880"
     CDS             complement(<1..1522)
                     /locus_tag="VFB89_06880"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZM27062.1"
                     /translation="MADLITRLQTALADRYTLERELGRGGMATVYLARDLRHRRPVAL
                     KVLHPELAYAVGGDRFTREIEVAASLDHPHILPLFDSGEADGLLYYVMPYVEGESLRD
                     RLKREGNLPLVDALQITREVADALAYAHSQQVVHRDIKPENILLSGGHARVADFGIAR
                     ALGQAGTDRLTGTGLALGTPAYMSPEQASGDHELDGRTDVYSLGCVLYETLAGEPPYS
                     GSTPQSVIMKRFTDRVPDVRRLRPEVPESVQQAISRSLAKDTGDRFSGAAELARALQP
                     KASLPTRPVAAHRLPSLPRFPHRQTIIVSTIIVVGLVVLFGMLSRERASTGPLDLNLV
                     AVAPFRVASADPSLAYLREGMVDLLASKLTGRGGPDAADTRSVLSAWRRASRSGGEDL
                     PQAEALKVAARVRCGLLLQGNVVGTPSRFTLNAAVLTVPEGRAKAQASVDGPADSLPV
                     MVDHLAAKLLALGAGEGEQRLGGLTSTSLPALKAYLEGQALYRRGKYAQAIRGFDEA"
     gene            complement(1610..1750)
                     /locus_tag="VFB89_06885"
     CDS             complement(1610..1750)
                     /locus_tag="VFB89_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27063.1"
                     /translation="MRFVEAKRVLCVSGVLSVKVHATPARPECYILLIVVATSDKPKT
                     FT"
     gene            1796..2320
                     /locus_tag="VFB89_06890"
     CDS             1796..2320
                     /locus_tag="VFB89_06890"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZM27064.1"
                     /translation="MAPINELERFRTVWDMEAHFTTQLLKSLPTDQYDFRPDPGGRSL
                     GELAWHLCEIEGYISDGIVKGAVTFQDAPPNLKRPREVAQLAPGYRRVHEEAVARLAN
                     LTEADLDREIQFADRRMTVRDILWGPILMHLVHHRGQLVVLCRLAGGKPAPIYGPNRE
                     EMAAMRERMRTANV"
     gene            complement(2257..>3002)
                     /locus_tag="VFB89_06895"
     CDS             complement(2257..>3002)
                     /locus_tag="VFB89_06895"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HZM27065.1"
                     /translation="DPRRAPDLVVENCASSGLRFDLGIMAHAHTAWLSDEVDPIASVA
                     LGYGCTVQFAPEVCNHWMVGDADSRVVDSSKPPGWWDFVLRVPMNGQFGISGRIPEWS
                     AVVRKRVADNVALYRRIRGAIAGADVYHLSPAPRRNDPTGWTALEYVQPTGTVESSWP
                     IAWRGAPPTRRSGCASSTPGRNTTSPWTGNDCRRPRVQIWPLVASEYASHDLPRRGHR
                     AHSATIAEPYTFAVRILSRMAAISSRFGP"
BASE COUNT          522 a          996 c          998 g          486 t
ORIGIN      
        1 gcgcctcgtc gaagcccctg atcgcctgag catacttgcc ccggcgatac agcgcctgcc
       61 cctcgagata cgccttcagc gccggcaggg acgtactggt caggccaccc aggcgctgct
      121 ccccctcgcc cgcgccgagc gcgagcagct tggcagcgag gtgatcgacc atgaccggca
      181 gactatcagc ggggccgtcg acgctcgctt gcgccttcgc gcgcccctct ggcaccgtca
      241 gtaccgcggc gtttagcgtg aatcggctag gcgttcccac cacgttgccc tgcagcagca
      301 gaccgcatcg cacccgcgcg gccactttga gggcttctgc ctgcggcagg tcttcgccgc
      361 cagatcgaga tgcccggcgc caagcgctca gaacggacct ggtatccgcc gcgtcggggc
      421 cgccgcgtcc ggtgagcttg gacgcgagca ggtccaccat cccttcccgc agatacgcga
      481 gggacggatc cgcactcgcc acccggaacg gggccactgc cacgaggttg aggtcgagtg
      541 gcccggtcga ggcgcgctcc ctcgagagca ttccgaatag cacgaccaag ccgaccacta
      601 tgatcgtgct gacgatgatc gtctggcggt gcgggaaccg ggggagtgat ggaagtcggt
      661 gtgccgcgac cggcctcgtg ggaaggctag ccttcggctg cagggcgcgg gcaagctcgg
      721 ccgctcccga gaaccggtcg cctgtgtctt tcgcgagcga gcgcgagatg gcctgctgga
      781 cgctttccgg cacctccgga cgcaggcggc gcacatctgg cacccggtcg gtgaaccgct
      841 tcatgatcac cgactgcggg gtcgagcccg agtacggcgg ctcacccgcg agggtctcgt
      901 aaaggacaca gcccaggctg tacacgtcgg tgcggccgtc cagctcgtga tcgccgctcg
      961 cctgttcggg gctcatgtac gccggcgtgc cgagcgcgag tccggttccg gtgagtcggt
     1021 ccgtgccggc ctgccccagc gcccgcgcga tgccgaagtc cgccacccgg gcgtgcccac
     1081 cgctcaacag gatgttctcg ggcttgatgt cgcgatgcac gacctgctgg ctgtgagcgt
     1141 acgccagagc gtcggcgact tcccgcgtga tctggagcgc gtcaaccagt ggaagattcc
     1201 cctcgcgctt caaccggtcc cgaagcgatt ccccttcgac atacggcatt acgtagtaca
     1261 gcaaaccgtc ggcctcgccc gagtcgaaga ggggcaggat atgcggatga tcgaggctgg
     1321 ccgcgacttc gatctcgcga gtgaaccgat caccgccgac ggcataggcg agctcgggat
     1381 ggagcacctt gagcgccacc ggacgccggt gccgtagatc gcgagcgaga tagaccgtgg
     1441 ccatcccgcc ccggccgagc tcgcgctcga gagtgtagcg gtcggctagc gcggtttgga
     1501 gccgcgtgat caggtcggcc atcaggcgca ttggaaggcg atgaaaggga actgagagcg
     1561 gtcgaatata tggccagggg cgggggtcag ctacgctatg ccatttccat caggtgaagg
     1621 tcttgggctt gtccgacgtc gcgacgacaa tgagcaggat gtagcactca ggccgtgctg
     1681 gcgttgcatg caccttgacc gacaacacgc cgctgacaca gagtacacgc ttggcctcaa
     1741 cgaatcgcac cgtccgcgcc taacgaaacg tctgcagact cgcaggagga tactcatggc
     1801 cccgattaac gaactggaac ggtttcgcac ggtctgggat atggaagccc acttcaccac
     1861 ccagctgctc aaatcgctcc cgaccgacca gtacgacttc cgacccgacc ccggtgggcg
     1921 ctcgctcggc gagctggcct ggcatctgtg cgagatcgag ggctacatca gcgatgggat
     1981 cgtcaaggga gcggtgacct tccaggacgc cccgcccaac ctcaagcgtc ctcgtgaggt
     2041 ggcgcagctt gcgcctgggt accggcgtgt gcacgaggag gcagtcgccc ggctcgcgaa
     2101 tctcaccgag gcagacctcg accgtgagat acagtttgcg gatcggagaa tgacggttcg
     2161 ggacatactg tggggcccga tcttgatgca ccttgtacat caccgcggtc agctggtggt
     2221 cttgtgccgc ctggcagggg ggaagccggc accgatctac ggaccgaacc gggaggagat
     2281 ggccgccatg cgggaaagaa tgcggacggc aaacgtgtag ggttcagcta ttgtcgcgct
     2341 gtgggctcga tgaccgcggc gcggcagatc gtgggaagcg tactcggagg ccaccagggg
     2401 ccagatctgc acccgtggcc ggcgacagtc gtttcccgtc cacggtgacg tcgtatttcg
     2461 cccgggcgtc gagctcgcgc agccggagcg tcgtgtcggt ggagctccgc gccaggcgat
     2521 aggccaggac gactccacgg tgcctgtcgg ctgcacgtac tccagcgcag tccacccggt
     2581 aggatcattt cgccggggcg cgggcgacag atggtagaca tcggcgcccg cgatggcccc
     2641 acgaattctc cgatatagcg caacgttgtc ggcaacgcgc ttccgcacca cggcgctcca
     2701 ctccgggatc cgcccggaga tgccgaattg cccgttcatc gggacgcgca gcacgaagtc
     2761 ccaccacccc ggcggcttgc tcgaatcgac gacacggcta tccgcgtcgc cgaccatcca
     2821 gtggttgcac acctcaggcg caaactgcac cgtgcagccg tagcccagag cgactgacgc
     2881 gatcgggtcc acctcgtcgg acaaccaggc cgtgtgggcg tgcgccatga tgccgaggtc
     2941 gaaacgcagg ccgctgctgg cgcagttctc caccacgagg tcgggtgcgc ggcgcggatc
     3001 gc
//
LOCUS       DASRJQ010000309         2747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3804537, whole genome shotgun sequence.
ACCESSION   DASRJQ010000309 DASRJQ010000000
VERSION     DASRJQ010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3804537"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..380
                     /gene="infA"
                     /locus_tag="VFB89_06900"
     CDS             159..380
                     /gene="infA"
                     /locus_tag="VFB89_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HZM27066.1"
                     /translation="MPTRDEKVELDGEVTEALRGRMFRVQLDNGHETLGYLAGKMKRY
                     RIRVFLGDRVRIEMSPYDLTRGRIVYRYR"
     gene            421..858
                     /locus_tag="VFB89_06905"
     CDS             421..858
                     /locus_tag="VFB89_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27067.1"
                     /translation="MSLPEQSSAHLPELFEIFIGSSRGHSYGACWDRPVVIYESFGPD
                     FEGREQVELAPSRAQWERFWRTMDEIGIWQWAERYEQVSPFEPTELGSAAAQWSVALA
                     HDGRQVCSSGTGAGPGSADLEESAAFDALLEAVSRLLGSRNFV"
     gene            complement(972..1283)
                     /locus_tag="VFB89_06910"
     CDS             complement(972..1283)
                     /locus_tag="VFB89_06910"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZM27068.1"
                     /translation="MSSGSDGKPWRFVTNHTQVLLCIARQGDMRLRDIAESVGITERA
                     AQRIVSDLAEAGFVTRERVGRRTHYTVHRTATMRHPSQADHHIGELLDLLKLDPPSQS
                     P"
     gene            1359..1709
                     /locus_tag="VFB89_06915"
     CDS             1359..1709
                     /locus_tag="VFB89_06915"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZM27069.1"
                     /translation="MDVRTHLESRADGTRVLRVAGELDLHTSPSFQEELQAAIEQPCE
                     SLLVDLTDCDYLDSSAIHVLVQAKRRLNGRADKLALIVPSPQLRRIFELAGLDEILHI
                     HPDQAAALPQNSNA"
     gene            complement(2071..2514)
                     /locus_tag="VFB89_06920"
     CDS             complement(2071..2514)
                     /locus_tag="VFB89_06920"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZM27070.1"
                     /translation="MQINRDAPIVSTKDGIIYATPDRVWEALADVSSWSAWHPEIRSA
                     ALQGAFEPGTQFRWKSGAATINSTLEVVEQPTLLGWVGRSTGLTARHMFHLHPDDGHT
                     RLTIEESMEGPTARLLKRFLLRMLDRSLEAWIAGLKRRTEAPTSS"
BASE COUNT          486 a          866 c          899 g          496 t
ORIGIN      
        1 aggacagcga gtcggttgcg ctgacggttc aactgtcaag tcgccagttc ttctttctcg
       61 tcgcgtccgg tactccgtag cgttttatgc tcggccaggc aaggcgcccg tgtggctgct
      121 tccgtgaggc cgggtcaccg cgagcccgag gagccgggat gccaaccagg gatgagaagg
      181 tcgagttgga tggtgaggta accgaggcgc tgcgaggtcg gatgttccgc gtccagctcg
      241 acaacgggca cgagactctc ggctacctcg ccggcaagat gaagcggtac cgcatcaggg
      301 tgtttctcgg tgaccgcgtc aggatcgaga tgtcgccgta cgacctcacg cgcgggcgca
      361 tcgtctatcg ctaccgatga tcgacccccg cctgtcacac aggggctgct gaaattcccg
      421 atgagccttc ccgagcagtc gagcgcgcac ctgccggagc tctttgaaat cttcatcggg
      481 agctcgcgcg gccactccta cggagcctgc tgggaccgac cggtagtcat ctacgagagc
      541 ttcggacccg acttcgaggg ccgcgagcag gtcgagcttg ctccctcgcg agcccagtgg
      601 gaacggttct ggcgcacgat ggatgagatc gggatctggc aatgggccga acgatacgaa
      661 caggtcagcc cgttcgagcc aaccgagctc gggagcgccg ccgcccagtg gtccgttgcg
      721 ctggctcatg acggccgcca agtctgctcg tctggcaccg gcgccgggcc tggctcagcc
      781 gaccttgaag agagcgcagc tttcgacgcc ctgctcgagg ctgtctcgcg tctgctcggc
      841 agccgcaact tcgtttgatc gagcaccgcc ggccgcggcg gcagatgccg gagctcgacg
      901 aaatggctga tcgacccgaa cctctagctc ggcgcagctt ggaagggccc catacgccct
      961 gcaagcgggt cttacggaga ttgggagggc ggatcgagct tgaggaggtc gaggagttcg
     1021 cctatgtggt gatcggcctg ggaggggtgg cgcatggtgg cggtgcggtg cacggtgtaa
     1081 tgggtgcgcc ggcccactcg ttcgcgggtg acgaagccgg cctcagcgag gtcgctgacg
     1141 attcgttggg ctgcccgttc ggtgatcccg acgctctcgg cgatgtcgcg caacctcatg
     1201 tcgccctgcc gggcgatgca cagcaagacc tgggtgtggt tggtgacgaa ccgccagggc
     1261 ttgccgtcgc tgccacttga catagaggac atatttgtca taaattatgt ttcgcgcttg
     1321 atctcacagg atagcactgt cgtgggagga ggtcgatcgt ggatgtacgc acccacctag
     1381 agtcccgggc cgatggaacc cgcgtcctgc gagttgccgg cgaactcgac ctgcatacca
     1441 gccccagctt ccaggaggag ctgcaggcgg cgatcgaaca gccctgcgaa tcgctgctgg
     1501 tcgacctgac cgactgcgac tatctcgact cgagcgccat ccacgtcctc gtccaggcaa
     1561 agcgacgtct caacgggcgc gcagacaagc tggcactgat cgttcccagc ccccagctgc
     1621 gcaggatctt cgaactcgcc gggctcgacg aaatactgca catccatccc gaccaagccg
     1681 ccgctctgcc acagaactcg aacgcctaag cgagcaagca gatggcgacc gtcgacagga
     1741 ccaccgtttc tggccgagtt gccttgcacg attaccgctg cgccacgtgc ggttacggcg
     1801 ttgccgtggc tcgcccacca gaatcctgcc cgatgtgcca gagcaaagtc tgggtacacg
     1861 cagagcaacc gcagctcgca gaggccagaa ataggccgag caaccccaag ttgacgaact
     1921 gacggggctg gcggccgggc cggcttcgag cgtcaggcca cagagctttc gccgtcgatc
     1981 gcagacaacc aacggcgctg tacgagcgtt gggctggagg aatcggttcc gcccggatcc
     2041 gattccttca tcgctgccag gcgaagccgc ctagcttgaa gtgggtgcct ccgttcgccg
     2101 tttgaggccg gcgatccagg cctcgagcga tcgatcgagc attcgcaaga ggaagcgctt
     2161 gagaaggcgt gcggtggggc cttccatcga ctcctcgatc gtgagcctcg tgtgcccgtc
     2221 gtcggggtga aggtgaaaca tgtgccgggc ggtaagcccc gtcgacctgc ctacccagcc
     2281 gagcagcgta ggctgctcta cgacctcaag cgtggagttg attgtggctg cgccgctctt
     2341 ccaccgaaac tgcgtgcccg gctcaaatgc tccctgaagt gcagcggagc ggatttccgg
     2401 gtgccacgcg ctccaggagc tgacgtcggc cagagcctcc caaacgcggt ccggtgtcgc
     2461 gtagatgatc ccgtccttgg tgctcacgat cggggcatcc ctgttgatct gcatcgggtc
     2521 actcccttca tggcggattg catcgcggct gacgagatcg accccatgac cgcgcgcggc
     2581 gctgatcacc tccaagatct cagcacgcgc ggcacttcga gctgagcggg agcgaagagc
     2641 gcgaaggggg cgtgtcaggt cgcgtctcgg cctaccgcga atccatcgga gacgttcggg
     2701 tagtcggggt ggtgctccgc gtacccgctc gcgcgataga cctcgag
//
LOCUS       DASRJQ010000310         2469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_4206127, whole genome shotgun sequence.
ACCESSION   DASRJQ010000310 DASRJQ010000000
VERSION     DASRJQ010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4206127"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1458
                     /locus_tag="VFB89_06925"
     CDS             <1..1458
                     /locus_tag="VFB89_06925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pls/PosA family non-ribosomal peptide
                     synthetase"
                     /protein_id="HZM27071.1"
                     /translation="YATWQLLTLLLVYLPVGLAGSSWLLLKVPRLSELMAEGAGAFTS
                     WTFYRDALVVSLVLYFGSILAGFVIVTTVPRLLNLLIRPDKVYRLYGFHYGIHRLIVR
                     LTNAKVLSNLVGDSSWIVHYLRGIGYDLGKIVQTGANFGGQVKHESPFLSSIGTGTMV
                     ADALSMMNAEYSTTTFRLSRTYIGERNYLGNGIAYPPRSKTGDNCLLATKVMVPISGK
                     VRENVGLLGSPAFEIPRSVDRDSSFDYLREGEEFRRRLAAKNRHNFATILWCLFTRWF
                     FIFMITVFTAIAGDFYASLGAVAIVLELVSAAVFGIAYWILVERIVTRFNPVRPLFCS
                     IYEPAMWRHERYWKVPATPYITAFVGTPFINMILRRVGSRIGRRVFNDGCAMTERSLV
                     TIGDDCTLNETSWIQCHSQEDGTFKSDYSTLGSNVTLGTGTLVHYGVTIGDGAELTTD
                     CFLMKGEQVPAGARWGGNPAHEIRDRQLVAASARG"
     gene            1470..>2469
                     /locus_tag="VFB89_06930"
     CDS             1470..>2469
                     /locus_tag="VFB89_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZM27072.1"
                     /translation="MDVLTETDRDYWCGVLSEGGSTAIPRWSDPAPNLWTSTCAVPSS
                     SKGSAQVAVYDAAIPEDLVAALRRLAVELNVPFSSMLVAAHAKVLAALSGERQVATGY
                     VGGTDPRPLPCRLTTEAESWRELLLDTHRVEAQLLSHADFPLDALRRELGVTVPSFET
                     VLDLTGADGDLGEDVVLRVGIAQPSDELVLRLRYRTDVLDADYAARIAGYHLTALELM
                     AADLDAEHQEQSLLSAEELYFQMEALAGPMRELPDRRVHELFEAQVEGHPDSIAAVHG
                     TRQWTYRELNARANRLARALLARGLGREGVVAVVTERNLDWLAAVLAVFKAGGVYLP"
BASE COUNT          411 a          748 c          826 g          484 t
ORIGIN      
        1 tacgccacct ggcaactgct gacgctgttg ctggtgtacc tgccggtggg actcgccggt
       61 agcagctggt tgctgctcaa agtgccgagg ctgagcgagc tgatggccga gggggccggt
      121 gccttcacca gctggacgtt ctaccgcgat gccctggtcg tgtccctcgt gctgtacttc
      181 ggctcgatac tcgccggttt cgtcattgtg acgaccgtcc cgcgcctgct caacctgctc
      241 atcaggcccg acaaggtcta ccggctgtac ggcttccact acgggatcca tcggttgatc
      301 gtgcgtctga cgaacgccaa ggtcttgagc aacctcgtcg gcgacagctc gtggatcgtg
      361 cactacttgc gcggtatcgg gtatgacctc gggaagatcg ttcagaccgg ggcgaacttc
      421 ggtggccagg tgaagcatga gagcccgttc ctgagctcta tcggcactgg aacgatggtc
      481 gccgacgcgc tgtcgatgat gaacgcggag tactcgacca ccaccttccg gctgtcccgg
      541 acgtatatcg gagaacgcaa ttacctcgga aacggcatcg cctatccgcc gcgaagcaag
      601 acgggggata actgcctgct tgcgacgaag gtcatggtcc cgatcagcgg gaaggtccgg
      661 gagaatgtcg ggctgctggg ctcccccgcc ttcgagatcc cgcgttcggt tgatcgcgac
      721 agcagcttcg actacctgag ggagggggag gagttccgcc gccgccttgc cgccaagaac
      781 aggcacaact tcgccactat cttgtggtgc ctgttcacgc gatggttctt catcttcatg
      841 atcaccgtgt tcacggcaat tgctggggac ttttacgcct cactgggtgc agtagcgatt
      901 gtgttggaac tcgtgtctgc cgctgtgttc ggtatcgcgt actggatcct ggtcgaacgg
      961 atcgtcacgc gattcaatcc cgtgcgacct ctgttctgct cgatctacga gcccgcgatg
     1021 tggcgccacg aacggtattg gaaagtgcct gcgacgccat atatcacggc tttcgtaggc
     1081 accccgttca tcaacatgat cctgcgccga gtgggtagcc ggatcggccg ccgggtgttc
     1141 aacgacggct gcgctatgac ggagcggtcg ctggtcacca tcggcgatga ctgcacgctc
     1201 aacgagacaa gctggatcca atgccactcg caggaggacg gcaccttcaa gtcggactac
     1261 tcgaccctag gctccaacgt caccctcggg accggcaccc tcgtccacta cggcgtgacg
     1321 atcggtgacg gcgccgagct caccactgac tgctttctga tgaagggcga gcaggtcccg
     1381 gccggcgcgc ggtggggcgg caacccggcc catgagattc gagatcgaca actggtcgcg
     1441 gcgagcgccc gcggatgagg ggaaattcca tggacgtact gactgagaca gatcgcgatt
     1501 actggtgcgg agtgctcagt gagggcggat ccaccgcaat cccgcggtgg agcgatcccg
     1561 caccgaatct atggacttcg acatgcgccg tcccgagctc gtcgaagggc tcagcccagg
     1621 tagccgtgta cgacgcggcg attcccgaag acctcgtggc ggcgttgcgc cggctggctg
     1681 tcgagctgaa cgtgccgttc agctcgatgc tggttgccgc gcacgcgaag gtgctcgccg
     1741 cgctgtccgg tgagcgccag gtcgcgaccg gctacgtcgg cgggaccgat ccccgaccgt
     1801 taccgtgccg gcttacgacg gaggctgagt cgtggcggga gctgctgctc gacacccacc
     1861 gcgtcgaggc gcaactgctg tcgcacgcgg attttccgtt ggacgcgctg aggcgcgagc
     1921 taggtgtgac ggtaccgagc ttcgagacgg tcctcgacct gaccggcgcg gatggcgacc
     1981 tcggcgagga cgtcgtgctg cgggtcggga tcgcgcagcc aagcgatgag ctggtattgc
     2041 gcctgcggta ccggaccgat gtgctcgatg cggactacgc agcccgcatc gccggctatc
     2101 acctcacggc gctggagctg atggccgccg atctcgatgc tgagcaccag gagcagagcc
     2161 tgctgtctgc ggaggagctc tacttccaga tggaagcgct ggccggtccg atgcgggagc
     2221 tgccggatcg ccgggttcat gagttgttcg aggcgcaggt ggagggtcat cccgacagca
     2281 tcgctgccgt gcacggcact cggcagtgga cctaccggga gctcaatgcc cgtgccaacc
     2341 ggttggctcg ggctttgttg gcgcgtgggt tgggccgtga gggtgtggtt gcggtggtga
     2401 ccgagcgcaa tctggattgg ctggctgcgg tgcttgcggt cttcaaggcc ggtggggtgt
     2461 atctgccga
//
LOCUS       DASRJQ010000311        10208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_874386, whole genome shotgun sequence.
ACCESSION   DASRJQ010000311 DASRJQ010000000
VERSION     DASRJQ010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 10208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10208
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874386"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="VFB89_06935"
     CDS             <1..1028
                     /locus_tag="VFB89_06935"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF005089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HZM27073.1"
                     /translation="RICIAGAGAIGGFMGARLSQAGHDVSLVTLGDHLTAIQCAGLRI
                     VGLDGQEDVITDAYATDDFGLLGEQDIVILAVKANHIETVAPSLPQLFHRDTIVVTVQ
                     NGIPWWYFERHGGPFQGRRIQCLDPHGFIGRHIPAYRIIGCVAYPAVETVAPGLLRHV
                     EGIRFPVGELDGSDTPRVRQLADLFMEAGFKSPVLSDIRGEVWLKAVGALAFNPISAL
                     THAYLGDLCRLPETRALALEMMREGEQVAARLGVTIRLPVERRLAGAEAVGAHKTSML
                     QDIEAGRPLEIEALLSAVRELGQLTDTPTPYIDAIYACAKLLDQTLTNAGARLRIDPI
                     DVADSVA"
     gene            1097..1561
                     /locus_tag="VFB89_06940"
     CDS             1097..1561
                     /locus_tag="VFB89_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27074.1"
                     /translation="MFSRSRLITTVVAIGAVAACASHDTTTSPSQFSMAGIWNQGARL
                     QDTTLLQTHIHTGYFFFDQEGNSFAGTGQQTGLCQGPLGDYVGPLATGLAFDITNGFQ
                     RGRNVSFQTDVCTYQGVMTADGSHIDGTARCEYTDQGVRFVWTGDWLANREP"
     gene            complement(1563..3059)
                     /locus_tag="VFB89_06945"
     CDS             complement(1563..3059)
                     /locus_tag="VFB89_06945"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZM27075.1"
                     /translation="MTRDRDGGIPRRTFVATLAAGAAVPWPRVFSVDPRVAQLTDDTL
                     HYSPVTVLAEAIRTKRVSSREVVNACIRRIEQVNPRLNAVVQLVAEAARADARQADAE
                     LARGKVRGPLHGIPFTVKDSFDVAGVISTAGTQGWAKRIPKEDATLVARIRAAGAILL
                     GKTNTPEFTWSDETDNLVYGRTSNPYDLARTPGGSSGGPAAILAAGGSPLDVGSDTGN
                     SIRMPAHNCGIAGIKPTAGRVSKYGHAISYRGILESWTQVGPMARRVEDLVLVLPLAA
                     GVDGKDPHAVPAPLREPARVNVRGLRVVTFTDNPLRKPTPETVRAVATAAATLRQAGA
                     RVEEQKPPGLDDATELWHKIAFADGGAWLHRLLKDAGTTGTGSLGEIPSKDEVPSGQL
                     SLLIEQLDEVRSQLLRYMQKVDLIVCPVMAMPAVLHGVAAKPGYADGYNEPHNITGWP
                     VAVVRVATSPENLPIGVQLVAQPWREDVALAAARVVESESGGWKPPPL"
     gene            3148..4545
                     /locus_tag="VFB89_06950"
     CDS             3148..4545
                     /locus_tag="VFB89_06950"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZM27076.1"
                     /translation="MSAGLPGRNELVKRDGQAQQMVVTSGPQPDRLPRQLGVWSAAAV
                     CVGSMIGSGIFRVPSAAAAATGTDTAMMLTWLVGGLVAVTGALSLAEVAALFPRAGGL
                     YVYLREAYGPLTSFLFGWLILIISPASTAAVALAFGEYVGRLVPALAGNERVMAVLSI
                     VVFAAANYRSVRYGAAIQNVLTSAKVLGIASLTLAAFTFSAAAPPSTVSDPPLPPATW
                     SGFGLGLVAVLFAYNGWQDATSIAGEVRDPGRALPRALIGGTLTVAIVYLAVNAAYLH
                     TLSMSEMARSPLVAAEMAVRLVGPIGAVVINALVCASTLGTVNVSMIVYPRIFFAMAE
                     DRLFFRGMAAVHPRYATPHPAIVLAAVFGVAYVTLRSFEQLIEIFILGSLPFWALAVA
                     AVPVLRWRRPDLPRIYRTPGYPVIPLFFVTAMLALIANSLREHPEAAGLTLGALLVGI
                     PIYLAWRWMQQRGRV"
     gene            complement(4556..5887)
                     /locus_tag="VFB89_06955"
     CDS             complement(4556..5887)
                     /locus_tag="VFB89_06955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZM27077.1"
                     /translation="MTAFSRAPYRTAGLLLLLLAVGAFLRIETLTKRPLWHDEIYTLG
                     VVVTAHSLADIWAASGRDRAETPLLYYGAAYAAWHLHRNEKSIRFPACIVGILSIPLL
                     GTLAYRLAGIRAGLLSALLLTVSVYHIEYSQDARSYTLLIFWSLTAFHGFLSFVRSRS
                     TAGLLVLTLSVLACLYTHHSAFFVGGAITIAGLAFTLRLWWQTGKFPLWLCGGLVGSA
                     LVITLGFWPQLAHFRSFLESSDLDREHTLALTGHFFVELFNRWTFGAPWGFIAVGLLA
                     PGAIRSCKSFERAVILFPWLAIPFLYFGFVPFGKFFDIRFVIAALPPFLILVGSGLSW
                     LSDGAATALARLGRLPDTSRTSLNAFMEALCVVFLVGSAVGPYLTFRRLELRCSDFYY
                     RHEVLHQEHGFCRKHLIYASLYRRDSAGMLRKYQPSSTRGSEKPAADSVPQ"
     gene            5994..7643
                     /locus_tag="VFB89_06960"
     CDS             5994..7643
                     /locus_tag="VFB89_06960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZM27078.1"
                     /translation="MMSPTTRACILSTAIAVGAAGVLDGIVAARMSPTPDEGGHIEFG
                     RRVLQGRPVRDRIYFDSKMPISALNAVPQLAADQLDSHHLLPRLATLLQRWKLARVAS
                     VLSLIVLNVLVFRWASELYGPAAGIAAAMLVVLAPNLIAHGTLATTDGYFALGVLLSM
                     YWLRQYLAQPTWKNACLSACAFAFAQLTKPFAIYLYAIAALGLAALKLGPGRGGKAPT
                     VRNIASYVALCLAAFVLVLNLGFAFDRSFMRFGSYRFTSAPLINLQQAVVRLPVLWRA
                     RVPLPYPYLQGLDMSADDEAHGKSYGNVYLLGQVGNSKDPQFHGFKSYYLVAWFFKEP
                     IALQLLLLFGLLEIRKRRSFTDFVLNEGLLLAASGALVFWLSFFSRAQLGIRHVLPAL
                     AANVVIAAAAFSRFPALSRGTKFGLSLLVVWLGISTLSYYPHLIPYMNEWVGDRRLAY
                     RLLADSNLDWGQNAYLVRDFLAKNPDVVLNPPTPVCGRVLINANQLTGVSPPRNWRGR
                     SWAWGYQPVEHIGYAHFLFHIPPDRADPNDVRGPDRCVGRP"
     gene            complement(7609..8970)
                     /locus_tag="VFB89_06965"
     CDS             complement(7609..8970)
                     /locus_tag="VFB89_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27079.1"
                     /translation="MSFAIFSWKPYWGRTANNHLLNTALMRWCSALFGNSELSLRLPN
                     LAAHALYLVCVLALVKRVRNPALKVFGFVLFALNLLLAEYFIVARGYGLATAFEMLSL
                     YLFVRAYEEGAKGKLARDLSLSVGSGALAVLANFAWLNFFIPLVLASAWLLATDASLR
                     QLNRQRISTTLVLLAGCGLFLWQVLARLLKLERAGQLYWGGYSGFISDTIHTLARCSL
                     FPGIDSPTTELAVSIAIIASFVVVLPLGLAQLRSGKNDVAFGILVLLLTGAVALPILE
                     HHLVHALFPIERAALYYLPLYAAVLVYGLDALSALPVERWRRAVTPISAIVTLAASGC
                     FLVGFTKRSSCAWLMDSHNREVLTLIDRDRTEQGLTGPVRVRTSHHMEPSFNFYRVTR
                     HYSWLVPVIRKPIPDPDYIYTLESELNPADRGVVLASYRDLRTVLIRVRHATDAQRSG
                     PGL"
     gene            9406..>10208
                     /locus_tag="VFB89_06970"
     CDS             9406..>10208
                     /locus_tag="VFB89_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27080.1"
                     /translation="MTNTQESAPAQRGNASPLARAGFAERRADLIVCALLLALWLAVV
                     IFTTTRHEYWRDEVRAWSLARAAQSPVDLFHLIRYEGHPALWYLILYIGHAIADSRLV
                     LPVASVAIAGAAITVFMLRSPFPLWLRALFLFSGLPLYEYSVMARNYGVSMLLLFMVA
                     SLYRYRTHHPLALATTLALLANTNIHSTILAFLLMVVWAWDEWRTRSSALDRRPARPM
                     FLAAVIVGLGILLGVVVVSPPRDTVLTHFYSKSPESLAFAFSAALFYPRT"
BASE COUNT         1886 a         3209 c         3157 g         1956 t
ORIGIN      
        1 tgcgaatctg catcgcaggc gcaggtgcaa ttggtggttt catgggcgcc cggctatccc
       61 aggcgggaca cgacgtcagc ctcgttacgc tgggcgatca tctcacggcc attcaatgcg
      121 ccggccttcg catcgtcgga ttggacgggc aagaggacgt gatcaccgac gcgtatgcca
      181 ccgatgactt cggcttgctg ggggaacagg acatcgtgat cctggcagtc aaggccaacc
      241 acatcgagac agtcgcgcct tcgctgcccc aactttttca tcgcgacacc atcgtcgtca
      301 cggtgcagaa cggaattccc tggtggtatt tcgagcgtca cggcgggccc ttccagggaa
      361 ggcgcatcca atgcctcgac ccccatgggt tcattggtcg gcacataccg gcatatcgta
      421 taatcggctg cgttgcctat ccagccgtcg agacggttgc gccgggcctg ctgcgtcacg
      481 tcgagggaat tcgatttcca gtcggggagc tcgacggctc cgatacgccg cgggtgcgcc
      541 aactggctga tctcttcatg gaagcggggt tcaagtcacc cgtcctgagc gatatccgcg
      601 gagaagtctg gctcaaagcc gtcggggctc ttgcgttcaa tcccatcagc gcgctcaccc
      661 acgcttacct gggcgacttg tgccggctcc ctgaaacgcg ggcccttgcg ctcgagatga
      721 tgcgggaagg cgagcaggtt gctgctcgac tcggcgtaac cattcggctg ccggtcgaac
      781 gacggcttgc cggcgccgag gccgtcggcg cccacaagac ttccatgctc caggacatcg
      841 aagccggccg cccgctcgag atcgaagcgc tcctgagcgc cgtacgcgag ctcggtcagc
      901 tcaccgatac gccgacacct tatatcgacg cgatttacgc ctgcgcgaag ttgctcgatc
      961 agaccctcac caacgcgggc gcccggctcc ggatcgatcc gatcgacgtc gcagattccg
     1021 tcgcctgaac gccttgtaag ttaccgcctg gcatggcacc ttagggacgc ggtcgagcag
     1081 ccctgaggag gacgctgtgt tttcgcgctc gaggctcatt acgacggtgg tggcgatcgg
     1141 cgcggtcgcg gcttgcgcat cccatgatac gacgacctcg ccctcgcagt tcagcatggc
     1201 gggtatatgg aatcaggggg cgcggctgca ggacactacc ttgctgcaga cccacattca
     1261 caccggctat ttctttttcg atcaggaggg caactcgttc gcgggaaccg gtcagcagac
     1321 cggtttgtgt cagggaccgc tcggtgacta cgtcgggccg ctcgcaaccg gcctggcctt
     1381 cgatatcacc aacggctttc aacgaggtcg gaacgtctcg ttccaaaccg atgtctgcac
     1441 ctaccagggg gtcatgacgg ctgacggatc gcacatcgat ggcacagcgc ggtgcgagta
     1501 caccgaccag ggagtgcgat tcgtctggac tggcgactgg ctcgccaacc gggagcctta
     1561 gctcacagcg gcgggggctt ccagccgccc gactccgact cgaccacccg cgctgccgcc
     1621 aaggcgacat cctcgcgcca cggctgcgcc accaactgaa ctccgatcgg caagttttcc
     1681 ggcgaggtcg ccactctgac gactgcgacg ggccagccgg tgatgttgtg gggctcgttg
     1741 tacccgtctg catatcccgg cttggccgcc acgccatgaa gaaccgccgg catcgccatc
     1801 acggggcaca cgatcagatc caccttctgc atgtaccgaa gcagctgcga tcgtacctca
     1861 tccaactgct cgatcaagag ggaaagctga ccagacggca cctcgtcctt gctcggtatt
     1921 tcccccagcg atcccgtccc ggttgtccct gcatccttca gcaaccggtg gagccatgcg
     1981 cctccgtccg caaaagcgat cttgtgccag agctcggtcg cgtcgtccag ccccggcggc
     2041 ttctgctcct cgaccctggc gcccgcctgg cgcagggtcg ccgctgccgt cgctacggct
     2101 cgaacggtct ccggcgtcgg cttccgaagc gggttgtcgg taaacgtgac caccctgagg
     2161 ccgcgcacat tcacccgcgc cggctctcgc agcggcgccg gcacggcatg cggatccttt
     2221 ccgtcgacgc ccgcggcgag gggcagcacc agcaccaggt cctctactcg ccgagccatg
     2281 ggcccgacct gggtccagga ctcgaggatg ccgcgataag agatggcgtg gccgtacttg
     2341 gacacgcgtc cggctgtcgg cttgatgcct gcaatgccgc agttgtgcgc cggcatccga
     2401 atgctgttgc cagtgtcgct cccgacatcc agtggcgagc cgccggctgc gagaatcgca
     2461 gccggcccac cgctgcttcc gccgggcgtc cgcgcgagat cgtacggatt cgaggtgcgg
     2521 ccgtagacca gattatcggt ttcgtcagac caggtgaact cgggggtgtt tgtcttgccc
     2581 agcaggatcg cgccggccgc ccggatgcgc gcgacgagcg tggcgtcttc tttggggatc
     2641 cgctttgccc atccctgagt accggcagtc gagatcacgc cggccacatc gaacgaatcc
     2701 ttgaccgtga acggaatgcc gtgcagcgga ccccgcacct tacctcgcgc cagctccgcg
     2761 tcagcctggc gggcatcggc gcgggctgct tcggcgacga gctggacgac ggcattgaga
     2821 cgcggattga cctgctcgat ccgccggatg cacgcgttca ccacttcgcg ggaggagact
     2881 cgcttggtgc gaatcgcctc cgcgagcacg gtaaccggcg agtagtggag ggtgtcgtca
     2941 gtcagctgag cgacacgcgg atcgactgaa aagacccggg gccagggaac ggcggcgccg
     3001 gcggccaggg tcgcgacgaa ggtgcgacga gggatacccc cgtcccggtc acgggtcacg
     3061 atcgaagcct ccgctattca ggctgtagcg ccgcaaggta ccggcagcca agccgttgtc
     3121 aacaacaggt gaaaggagcg cccctcagtg agcgctggat tgcctggtag aaacgagctg
     3181 gtcaagcgag atggtcaagc acaacagatg gtggtgacct ccggcccgca gcccgaccgg
     3241 cttccacgcc agctgggcgt ctggtcggca gctgcggtgt gcgtgggcag tatgatcggc
     3301 agcggcattt tccgcgtgcc gagcgccgct gcagcggcca caggaacgga cacagccatg
     3361 atgctcacct ggttggtggg cgggctggtt gccgtgaccg gcgcattgag cctcgccgag
     3421 gtcgcggcac tctttcctcg cgcgggcggc ctgtacgtct acctgcgcga agcgtacggg
     3481 cctctcacct cgttcctctt tggctggctg atcttgatca tcagtccggc ttcgacggcc
     3541 gcggtggcgt tggcgttcgg cgagtatgtg ggtcgcctgg tgccggccct ggccggcaac
     3601 gagcgtgtga tggcggtgct gtcgatcgtg gtattcgcgg cggcgaacta tcgatcggtc
     3661 cgctacggtg cggcgattca gaatgtcctt acttccgcga aggtcctggg aatcgcgtcc
     3721 ctcacccttg ccgccttcac gttctcggct gcggctccgc cgtcgacagt cagcgatcca
     3781 ccgctcccgc ccgcaacgtg gagcggcttc gggctcggcc tggttgcagt cctgttcgcc
     3841 tacaatggat ggcaggacgc gacatccatc gccggcgagg tgcgcgatcc cggtcgtgct
     3901 cttccgagag cgctgatcgg cggaacgctc acggtggcaa tcgtgtacct cgccgtgaat
     3961 gctgcttacc tgcacaccct ctccatgtcg gagatggcga gatcgcccct ggtcgcggcc
     4021 gagatggccg tgcggctggt tggaccgatc ggcgctgtgg tcatcaacgc gctggtctgt
     4081 gcgtccacac tcggcacggt caacgtgtcg atgatcgtct atccacgcat attctttgcg
     4141 atggcggagg accgattgtt cttccgcggc atggccgctg tgcatccccg gtatgccacg
     4201 ccgcaccccg ccatcgttct cgccgcagtc tttggtgtag cctacgtcac gctccgaagc
     4261 tttgagcagc tgatcgagat cttcattctg ggaagcctgc ccttctgggc cctcgccgtt
     4321 gctgcggtcc ccgtgctgcg gtggcgccgg ccggaccttc cgcggattta tcggacgcca
     4381 ggatatcccg tcattccgct tttctttgtc acggccatgc tggccctgat cgcaaactct
     4441 ctccgagagc acccggaggc ggccgggttg acactgggag cccttttggt cggcatcccc
     4501 atctacctcg cttggcggtg gatgcagcag cgcgggcgtg tttgaccgga gaggcctatt
     4561 gggggactga atccgccgct ggtttctccg agccacgcgt ggacgacggc tgatatttcc
     4621 tcagcatacc agccgagtcc cttcggtaga gcgaggcgta tatcaggtgc tttcgacaaa
     4681 acccgtgctc ctgatgcaag acctcatggc ggtagtaaaa atcgctgcag cgaagctcca
     4741 atcgtcgaaa cgtcaggtac ggtcccaccg cgctcccaac caggaacacc acgcagagag
     4801 cttccatgaa agcgttgagc gacgttcgac tggtatccgg tagccgaccc aatcgtgcca
     4861 gagccgtagc cgctccatcg ctcagccagc tcagaccgga tccgaccagg attagaaacg
     4921 gtgggagcgc ggcgatcaca aagcgaatgt cgaagaattt accgaacggc acgaacccga
     4981 agtacaggaa tggtatcgcc agccagggga acaggatcac cgccctctca aatgacttgc
     5041 agctcctgat tgcccccggc gcaaggagtc cgaccgcgat gaatccccac ggagctccaa
     5101 acgtccaccg attgaacagc tcgacgaaaa aatggccggt gagcgcgagg gtgtgctccc
     5161 gatcgagatc ggagctctcc agaaaactcc tgaaatgggc gagctgcggc cagaaaccga
     5221 gtgttatcac cagggcggag ccgacgaggc cgccgcaaag ccaaaggggg aacttcccgg
     5281 tttgccacca gagccggagt gtgaatgcaa gacctgcaat ggtgattgct ccgccgacga
     5341 agaacgccga gtgatgggta tacagacagg cgaggacact gagtgtcagg accagaaggc
     5401 cggcggtgct cctggaccga acgaaagata gaaagccgtg gaatgccgtc agcgaccaaa
     5461 agatcagcag cgtatacgag cgggcatcct gagaatactc aatgtggtat accgatacgg
     5521 tcaggagcag agcggacagg agacccgctc tgattcccgc gagccgatac gcgagcgttc
     5581 ccaggagggg gatggacagt attcccacga tgcaggctgg aaagcggatc gatttctcat
     5641 ttcggtgaag gtgccacgcc gcatacgcag ccccatagta gagcaacggt gtctcggcac
     5701 ggtcgcgccc cgacgctgcc cagatgtcgg caagtgagtg tgcggtgaca acgacaccca
     5761 gcgtatagat ctcatcgtgc cacagcggac gcttggtgag ggtttcgatc cggagaaacg
     5821 cgccgaccgc cagcaacagg agcaacaacc cagccgtgcg atatggggcg cgcgaaaatg
     5881 ccgtcatcgt gtctcgccga gaacgtggcc gacgttaagt tatctgcgaa aatgcctgca
     5941 actcacaggt gtggcccgtc ggactcccct aacgcggcga gaggctgcgt ttcatgatgt
     6001 ctcccacaac acgggcctgc atcctatcca cggcaatcgc cgtcggagct gccggcgtgc
     6061 tcgacggaat tgtggccgcc cggatgtcgc ctacaccaga cgagggcggt catatcgagt
     6121 tcggccgcag agtcctgcag ggtcgaccgg ttcgagaccg gatctacttc gacagcaaga
     6181 tgccaatctc ggcgttgaac gcggttcctc agctcgcggc cgaccagctc gactcccatc
     6241 acctgctccc ccgactggca acactgctcc agcgctggaa gctcgcccgc gtggcgagcg
     6301 tcctgagcct gatcgtgctc aacgtgctcg tcttccggtg ggccagtgag ctttacggcc
     6361 cggcggccgg catcgccgct gcgatgctcg tggtgctcgc accgaacctc atcgcccacg
     6421 gaacgctcgc gaccacggac ggttatttcg ccttgggggt actcctgtcg atgtactggc
     6481 tccgccagta tctcgcccaa ccgacctgga agaacgcgtg tctgagtgcg tgtgctttcg
     6541 ccttcgcgca gctcacgaag ccgttcgcga tctacctgta tgccatcgca gccctggggt
     6601 tggccgctct caagctcggt cctggccgcg gcggcaaagc gccgaccgtt cgaaatatcg
     6661 cgtcgtacgt cgctctatgc ctggcagcct ttgtcctggt cctcaacctg ggctttgcct
     6721 tcgaccggtc gttcatgcgg tttggatctt atcgctttac aagcgctcca ctgatcaacc
     6781 tccagcaagc cgtggtgcgt ttgcctgtac tgtggcgtgc tcgggttccg ctgccgtacc
     6841 catacctgca gggcctcgac atgtcggccg acgacgaggc gcacggcaag agttatggga
     6901 acgtgtatct cctgggccaa gtcgggaaca gcaaggaccc gcagtttcac gggttcaaga
     6961 gctactacct ggtcgcctgg ttcttcaaag agcccatcgc acttcaattg ctgcttcttt
     7021 tcggcctgct cgagatccgt aagcgccgtt cattcactga tttcgtactc aatgaaggtc
     7081 tgctacttgc agcgtctggg gcactcgttt tctggctctc gttctttagc cgggctcagc
     7141 tcgggatccg acatgtcctg cctgctttgg ccgctaacgt agtgattgct gcggcagcgt
     7201 tctctcgctt tcctgctttg tcgcggggca ccaagtttgg actcagcctc ctcgtggtct
     7261 ggcttggcat ctccacgtta agctattacc cgcatctcat tccctacatg aacgaatggg
     7321 tcggcgaccg gcgtctcgcg tatcgcttgc tggcagacag caacctcgac tgggggcaga
     7381 acgcctacct ggttcgcgac ttcctggcga agaatccgga cgtagtattg aatcccccga
     7441 caccggtctg cggtcgcgtc ctgatcaacg ccaaccagct caccggagtc tcgccgccga
     7501 gaaactggag ggggcgttcg tgggcctggg gataccagcc ggtcgagcat atcgggtacg
     7561 cacacttcct gtttcacatt ccgcctgatc gggcggaccc caatgatgtc agaggcccgg
     7621 accgctgcgt tgggcgtccg tagcgtgacg tacccggatg aggacggtgc gtagatcccg
     7681 ataggaagcg aggacgacgc cacgatctgc cgggttcagc tcgctttcca aggtatagat
     7741 gtagtccgga tcgggaatgg gctttcggat caccggtacc agccaggaat agtggcgagt
     7801 cacccggtaa aagttgaacg acggttccat atgatggctc gtgcgaaccc tcaccgggcc
     7861 cgtcaatccc tgctccgttc gatcacgatc gatcaaagtc aggacctccc gattgtggct
     7921 gtccatgagc caggcgcacg agctacgctt ggtaaatccg acaagaaagc atccgctggc
     7981 cgcgagcgtg acaatagccg aaatgggagt aacggcgcgt ctccatctct cgacgggtaa
     8041 cgctgagagc gcgtcgagac cgtacaccag gacggccgcg tacagaggca ggtagtagag
     8101 ggctgcgcgc tcaatgggga atagcgcgtg aacgagatga tgctcaagta tggggagggc
     8161 caccgcgccg gtgagcagga gcaccaggat tccaaatgcg acgtcgtttt ttccggaccg
     8221 gagctgtgca aggccgagcg ggaggacgac cacgaatgac gcaatgatgg cgatcgaaac
     8281 tgccagctca gtggtgggtg agtcgatacc aggaaacagc gagcaacgcg caagggtgtg
     8341 gatcgtgtcg ctgataaaac cgctgtatcc accccagtag agttgtccgg ctcgctcgag
     8401 cttcaggagc ctggccagga cttgccagag gaagagtccg catccggcga gaagcacgag
     8461 tgtggtcgag atccgttgcc gattgagctg gcgcagcgag gcgtcggtag ccagcaacca
     8521 ggcggacgca agcactagag ggataaaaaa gttgagccag gcgaaattcg cgagtaccgc
     8581 aagggccccc gacccgaccg agagggacag gtcacgggca agtttccctt tcgcgccctc
     8641 ttcgtaagcc cgcacgaaga ggtagagact cagcatttca aatgccgtcg cgagcccgta
     8701 cccgcgggct acgatgaaat actcggcgag aagcagattg agagcgaaga ggacgaaccc
     8761 gaacaccttg agggcagggt tcctcactct tttgaccagg gcgagcacgc aaacgagata
     8821 cagggcgtgg gctgcgagat tcggcaggcg caacgagagc tccgagttgc cgaatagcgc
     8881 ggagcaccaa cgcatcaagg cggtgttgag tagatgattg ttggcggtgc gtccccagta
     8941 cggcttccag ctgaaaattg caaagctcaa actttcatcc gcgccaaaag acagctttgc
     9001 cgcccggtag accgaggagg caagaaggcc gatgcttgtg agaatcagga gggtccagag
     9061 agcccgatgc ctcgttcctg gctggccatc gtgtcccact gaggtcattc gcgctgaccc
     9121 gacgtggtac gcatccgggg ctgggcttgc cgttgaacat acccgggagc ggcgtccatg
     9181 gatacactca gcgggcagaa aggtgcgggc agtgtactac cccgaagccc cgccaggcaa
     9241 cgcggcgatc gggaaccaaa tcctcgggag ctgggagccg tgctgcgaga tcgcgtacat
     9301 tggtgtcatg ccccaacccg gcttgcccct cttagttcat ctggtagtcc tttcatcgcc
     9361 ggcctcatgg cagtgggtcg cccgcgtccc tgctaacccc aatacgtgac caatacacag
     9421 gaatcggcgc cagcccagcg ggggaacgcc tcgccactgg cgcgcgcggg attcgcggag
     9481 aggcgagctg atctcattgt ctgcgcgctc ctgctcgctc tttggctcgc ggtcgtcatt
     9541 ttcaccacca ctcggcatga atactggcgg gacgaggtac gggcgtggtc tctggcgcgg
     9601 gctgctcaat caccggtgga tctcttccac ctgatccgat acgagggcca tcctgccctg
     9661 tggtacctga ttctctacat cggccacgct atcgccgaca gccggctggt gttgccagtc
     9721 gccagcgttg ccatagcggg cgccgccata acggtgttca tgctccgttc ccccttcccg
     9781 ttatggctga gagcgctgtt tctcttcagt ggcttgccac tctacgagta ctcggtcatg
     9841 gcgcggaact acggcgtcag catgctgctg ctgttcatgg ttgcatcgct ctaccgttat
     9901 cggacccatc acccgctggc gcttgcgacc acgctcgcac tgctggcgaa cacgaacatc
     9961 cattcgacga tcctcgcgtt cctgttgatg gtggtctggg cgtgggacga gtggcgtacc
    10021 cgtagctctg ctctcgaccg aagaccggcc cgaccaatgt tcctggcagc tgtcatcgta
    10081 gggctcggca ttttgctggg cgttgtagtg gtgagccccc cgcgagatac agttctgacc
    10141 cacttctact caaagagccc tgagtcgctc gcgttcgcgt tcagcgcggc gctgttctat
    10201 ccgaggac
//
LOCUS       DASRJQ010000312         5959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3386922, whole genome shotgun sequence.
ACCESSION   DASRJQ010000312 DASRJQ010000000
VERSION     DASRJQ010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5959
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3386922"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2821
                     /locus_tag="VFB89_06975"
     CDS             <1..2821
                     /locus_tag="VFB89_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM27081.1"
                     /translation="GAEAETHFGLVHSSPPYRARTSPARVHCATSSVQLWYFEPSSAK
                     LSWRRPHMDAIARLAPALAERYRIERELGAGGMATVYLADDLRHGRKVAIKLLHAELS
                     AMLGPERFLTEIKTTAALQHPHVLPLFDSGSTDGLLYYVMPFVEGETLRHRLERERQL
                     PVGDAVRIATEVADALEYAHQHGIVHRDIKPENILLQSGHALVADFGIALAVAEAGGN
                     RLTQTGFSLGTPQYMAPEQATGEKHVDPRADIYALGAVTYEMLAGAAPFIGPTAQAVV
                     VKVITERARPLRELRDTVPPHVAAAVQSALEKLPADRPASAAAFARMLADEGTRTLAV
                     PAVPSRSRWTWPASGIAALVLVAAAGVGYAIGHRPTPERAPGFPPSRLALLAPTLGGS
                     GAAGLHRQIALTPDGEAVIYVTENPNGDNSLALQRLDAAEPAMIPGGDGLMDPRYSLD
                     GRWIMTWGFPVLRATERAFRLPAAGGTPSQLPVGVDSRFGAWSADGSFWFTSINSGAI
                     QRLSPDGQVTTVFPQRTVGLRVQQVIEPGLWGLAVRAPLGNTAGPLVMFGLESGQETT
                     VLDVPVVEARYTTGLLVYAQSDGILRAVPFDPRAQRTTGTPVQIASGVSLTGSGVAQF
                     AVAANGTVAYIPEDPRSLVFADRSGAFRLATAEGHNYHAPEFSPDGRRVSVDFTGGDG
                     RDVWVLSLVQGTLSRATFDRDGHDATWTPDGKYLTYTSFRSGTFGLYRARIGSAEPPE
                     SLLASPVLGYTGRWLTDGSGLLTTGSDLNPTSGADIVLVRNRGRGPIERVVANQFQTA
                     YPMPSPDGKWFAFVSNQSGEEEVFVRSLDQRGEEIQVSQGGGSEPVWSPDGRELYYRG
                     NAEGRIKLMAATIGGAPGLEVLSRTALFPVTDIVGATPHANYAISPDGKTFVMVRRAP
                     ANRIIVLQNLSELVRRIQGAGSVAR"
     gene            2959..5934
                     /locus_tag="VFB89_06980"
                     /pseudo
     CDS             2959..5934
                     /locus_tag="VFB89_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_271413140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
BASE COUNT         1026 a         2069 c         1879 g          985 t
ORIGIN      
        1 tggggccgaa gcggagactc acttcggcct cgtccattcc tcgcctccct accgggcccg
       61 gacatcgcca gcgcgggtcc attgcgccac ctccagcgtg caactatggt atttcgaacc
      121 ctcttccgcc aaactgtcct ggcgccggcc acacatggac gcgattgccc gccttgctcc
      181 tgctctggcg gagcgctacc gcatcgagcg cgagcttggt gcgggcggca tggccacggt
      241 ctacctcgct gacgatctcc gtcacggccg caaggtcgcc atcaaactcc tacacgccga
      301 gctttccgcc atgctcggcc cggagcggtt tctcaccgag atcaagacca cggccgcact
      361 gcagcacccg catgtcctgc cgctcttcga ctccggctcg accgacggac ttctctacta
      421 tgtaatgcct ttcgtagagg gcgagaccct ccgccatcgg ctcgagcgcg agcgccagct
      481 cccggtgggc gacgctgttc ggatcgcaac cgaggtcgcc gatgcgctcg agtacgccca
      541 ccagcacgga atcgtccacc gcgatatcaa gccggaaaac attctcttgc agagcggcca
      601 cgcattggtg gccgacttcg gcatcgccct cgccgtggcc gaggccggcg ggaatcggtt
      661 gacccagacc gggttttccc taggcacgcc acagtacatg gcgcccgagc aggccaccgg
      721 ggagaagcac gtcgatccgc gcgccgacat atacgcactc ggcgctgtga cctacgagat
      781 gcttgccggg gcagcgccgt tcatcggtcc caccgcgcag gcagtcgtcg tcaaggtcat
      841 cacggaacgc gcccgcccgc ttcgtgagct gcgcgatacg gtgccgcccc atgtggcagc
      901 ggcggtgcaa tcggcgctcg aaaagctgcc ggctgatcgt ccggcgagcg ccgcggcatt
      961 cgcccggatg ctcgcagatg aaggcacccg gaccttggcg gttccagccg tgccgagccg
     1021 ctctcggtgg acctggcccg cgtcgggtat cgcggcattg gtgctggtgg ccgccgcggg
     1081 cgtcggatac gcgattggcc atcggccgac accggaaaga gcgccgggct ttccgccgtc
     1141 acggcttgcg ctccttgcac ccactctggg cggcagtggc gccgccgggc tccaccgaca
     1201 aatcgcgctc acgccggacg gtgaagcagt catttacgtc accgagaacc ccaacggcga
     1261 caattcgctc gccttgcagc gcctcgatgc ggcagagcct gccatgattc cgggcggcga
     1321 cgggctcatg gatccgcgct actcgctcga cgggcgctgg atcatgacgt ggggctttcc
     1381 cgtactcaga gcgaccgaac gggccttccg cctccctgcc gcaggcggca ctcccagcca
     1441 gcttcccgta ggcgtggatt cccgcttcgg cgcatggagc gccgacggga gcttctggtt
     1501 cacctccatc aactccggcg ccatccaacg cctctcgccc gacgggcaag tcacgacggt
     1561 ctttcctcag cgtacggtcg gactgcgcgt gcagcaggtc atcgaacctg ggttgtgggg
     1621 actagccgtg cgcgcaccgc tcgggaacac ggctggcccg ctcgtgatgt tcggcctcga
     1681 gagcggccag gagaccaccg tgctcgacgt gccggtggtc gaggcgcgct ataccacggg
     1741 gcttctggtt tacgcgcagt cggacggtat tctcagggcc gtgccattcg acccccgggc
     1801 gcagcgcacg accggcacgc cggtgcaaat cgccagtggc gtctcgctca cgggcagcgg
     1861 cgttgctcag ttcgcggttg cggccaacgg cacggtggcc tacatccccg aggacccgcg
     1921 gtcgcttgtc tttgccgacc gctcgggcgc cttccggctc gcgacagcgg aaggacacaa
     1981 ctatcacgcg cccgagttct cgcccgatgg ccggcgcgtt tcggtggatt tcaccggggg
     2041 cgatggacgc gatgtatggg ttctgtcact ggtacagggc accctctccc gcgccacctt
     2101 cgaccgcgat gggcacgatg cgacttggac cccggacggg aagtacctca cctacacgag
     2161 cttccgaagt ggaaccttcg ggctctaccg cgcccggatc ggaagcgcgg agccgcccga
     2221 gtcgctgctc gcatcaccgg tgctgggtta taccggaaga tggcttaccg acggcagcgg
     2281 cctgctcacg accggctcgg atctgaaccc cacgtccgga gcggacattg tgctcgttcg
     2341 gaaccgcggc cgtgggccga tcgagcgcgt ggtcgccaac cagttccaga cggcctaccc
     2401 aatgccatcg ccggatggga aatggttcgc gttcgtctcc aaccagtcgg gcgaggagga
     2461 agtgttcgtc cgctcgctcg atcagcgagg cgaggagatc caagtctcgc agggcggcgg
     2521 cagcgagccg gtttggtcgc ccgatgggcg cgagctctat tatcgcggca acgcggaagg
     2581 acggatcaag ctgatggcgg cgacaattgg gggggcgcca gggctggagg tgctctcgcg
     2641 gacggcgctc ttccccgtga ccgatattgt cggcgctacg ccgcacgcga actatgcgat
     2701 ttctcccgac ggcaagacat tcgtgatggt gcgccgggcg ccggccaatc gcatcattgt
     2761 gctgcagaac ctgtcggagc tggtacgtcg cattcaaggg gccggctcag tagcgcgttg
     2821 aacggcgaaa ggcagcctta cctgcgtacg tgaccggcgc gatcgtcgcg gcgagcacca
     2881 tcacatcgca agagcgttct agcttttgtc ctggagcaac gtcaatgctg cgggttcgcc
     2941 ttcctgggcc cggttcgcat gacaaagtcc ccccttccct ctttcccgca ctcaccgccg
     3001 tcgcacgagc ccttcagtcg gcaaacggcc cgaaagacgt cgcgactccg cggcgccacg
     3061 gtcaccgaag gcctctgccc ctactgcgcc gtcggctgcg ggcagctgat ctacaccaag
     3121 ggaggacagc tcatcgacat cgagggtaac ccggacagcc ccatcaacga gggtaccctc
     3181 tgccccaaag gcgccaactc ctttcagctc gccgtcaatc cgcaccgagt cacccaagtg
     3241 ctctaccgcg ccccatactc cgaccactgg gagactaggt cgctcgactg ggccctcgac
     3301 cggatcgcgc agcgggtaaa ggaaacgcga gatgccgact tcaccgtccg ggatgagacc
     3361 ggccgggtgc tcaactcggt ccgctccatg ggtaccctgg gcggagccac gatcgacaac
     3421 gaggagaact acctcatcaa gaagcttttc ggtgcggggc tcggcgtcgt ctccatcgag
     3481 aaccaggccc gaatatgaca tagcgcctcg gtgcccggtc tgggcgcctc gttcggtcgc
     3541 ggcgcggcca ccacctacca gcaggacctg gcgaacagcg attgcattct gttcatgggt
     3601 tccaacatgg ccgaggccca cccggtaggc ttccgctggc ccatgaaggc gaaggaacgc
     3661 ggcgcgactc tcatccacgt ggatccgcgc tttacccgca cgtcggccct gtgcgacctt
     3721 cacatcggta tccgggccgg cagcgacatc gccttcctcg gcggactggt ccactacgtc
     3781 ctgagccatg atcggtggtt caaggagtac gccctccatt acaccaatgc agcgaccatc
     3841 ctccaggagg gattcaagga caccgaggaa ctgggtggcc tgttcagcgg ctaccacggg
     3901 aaccggaaga ccggtaccta cgatgcccgg gaaggtcgct ggggatatgt cggcacgcct
     3961 gcgccggaag agcatgaaga gtggcgcggc gctaccccca tgtaccagat gctgcggcac
     4021 gagctcggga tgcatgggca cagcatcgag cagggcgctc cctgccactc gggccctcgc
     4081 cacgagctga tgacaatgga agggcgccga ccggtaacgg atccgacgct gcaacatccg
     4141 cgctgcgtgt accagattct caagcgccac ttcgcccgct acacacccga gttcgtgtcc
     4201 caggtatgcg gctgttcccc cgaagcgatg gtgcgggtgg cggagctcct ctgcgccaac
     4261 tccgggcgcg accgaaccag cgcgatcgtt tacgcgctgg ggtggactca gcacaccacc
     4321 ggtgtccaga tgatccgcac cgcaggcatc ctgcaggcgt tgctcggaaa catcggccgg
     4381 cccggcggtg gaatcatggc gatgcgcggc cactcaacca tccagggctc gactgacctc
     4441 gcgaccctct acgacgccct gcctggttac ctgccgcagc cctgcagcga cgagctgcac
     4501 aacaatctcg agagctacgt ggcccatgag ggcctggcga ccggttactg ggccaatttt
     4561 cgcaagttca tagtcagcct gctcaaggca tggtacggcg acgcagcgtc ctgggagaac
     4621 gacttccgct actcctggct cccccgcgtc gatgccgact actcgcacct gccgtatttc
     4681 gaccgcatgg ccaagggcca ggtcaagggc tacttcctct tcggccagaa cccggccggc
     4741 ggcgcaccca acgccggact gcaccgcgcc gggcttcgca agctcgactg gctcgtcgtg
     4801 gtggactggt tcgagatcga gagcgcggtt ttctggaagg acgacccgac cgggccgccg
     4861 cctgagcaga tcgggaccga ggtgttcttc ctcccggctg ctgccgctcc cgaaaaggac
     4921 ggcagcctca ccaatactca gcgcttgctc cagtggcacg acaaggctct cgaccctccc
     4981 gccgattgtc gctccgacgc ctggatcctc tacaatctcg gcaagcgcct caagcagctc
     5041 tacgccggct ccaccgagcc gagggatcag cccctgctcc aactcacctg ggattacgag
     5101 ttcgacgagc atccgcgcct gcccgacggc aggcggagcc ggatcgaagg cgaaccggat
     5161 cttgagaagg tgctgatgga gatcaacggc ttcaggctgg acgacctcga tccgcgcacc
     5221 ggccggccac gcctgctcag ggaattctcc gagctcgagg acgacggcac gaccgcctgc
     5281 gggtgctgga tctacagcgg agtcttcccc cagccaggcc gcaaccgcgc gcgggagcgc
     5341 aagcgcacgg gccacccact cgagcctgag tggggatttg cgtggcccca caaccggcgg
     5401 atcctgtaca accgcgcctc tgccgacccc gagggccgcc cctggtccga gcgcaaaaag
     5461 ctgatctggt ggagtgagga gcagcgtcgc tgggtgggcc tggacgagcc cgactacgac
     5521 ccgaacacgc ggcccgatta cgagccgcct ccaggcgccc agggcatggc ggcgatcgcg
     5581 ggcacgcagc cgttcatcat gaagcccgac ggagtggcct ggctctacgc tcccggcgga
     5641 gtccgcgacg ggccgcttcc ggtccactac gagccgatcg agtcgccggt gggcaacctg
     5701 ttgtatccga agcagaccgg caacccgacg gtgcgcactt tcgagggacc gctcaaccac
     5761 ctggcccacg ctccgaccac ggagtatccg gtagtggcca ccacgtttcg gctcacggag
     5821 cactacttga gcggcccgat gagccgcttc aacagctggc tcaacgagct aatgcccgag
     5881 atgttcgtcg agttgagccc ggagctggcc gccgagcggg ggatcgtgca cggcggttgc
     5941 tgacggtgcg ctcggcgcg
//
LOCUS       DASRJQ010000313         5992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3818594, whole genome shotgun sequence.
ACCESSION   DASRJQ010000313 DASRJQ010000000
VERSION     DASRJQ010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3818594"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..773)
                     /locus_tag="VFB89_06985"
     CDS             complement(183..773)
                     /locus_tag="VFB89_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZM27082.1"
                     /translation="MGRLLSAVESTIAERGVASLTVQEVARRADIAVGTLYTRFPGKD
                     ALLRAFTIAFFDRARRAADALLDDPRWRRLPPRELVAAVVKALVRSYRAKRRLLRALH
                     LFVRSHPDASFQAEAAAFNAEFVRRLTVLLLQHREAMAHPDPERGVLLGFLLVDAAAK
                     EAILFDDARPPDLRVADEELIEALTLSYCQLLDFVA"
     gene            996..1745
                     /locus_tag="VFB89_06990"
     CDS             996..1745
                     /locus_tag="VFB89_06990"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HZM27083.1"
                     /translation="MKIAVCLKRVPDTVAKITIGADRKSIDESGLKFVPNPYDEFAIE
                     EAIALKEKAGSGETVVYGIGTDAAQETLRAALAMGIDRAVLLKSPGSPDGLEIARVLA
                     AEVKEGGYDLILCGKLAVDDYNHQVGVMLAELLGLPCISSVAHLTVADGSVEAEREIE
                     GGVEVATCQLPAVLTCEKGLNNPRLPSLKGIMAAKKKPLETKAVPVGVGSIEVLSLDY
                     PAERKPGKVVGEGADAVPELLRLLRTEARVL"
     gene            1747..2721
                     /locus_tag="VFB89_06995"
     CDS             1747..2721
                     /locus_tag="VFB89_06995"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HZM27084.1"
                     /translation="MPANAILAVLETRDGALRKVSHEVVAAARQISTAGGSVDALAFG
                     VSAPEGSNGVGANRVLVAAHAEFGRYNPDGFAATVASLAAGYGAIVFAATATGRDLAP
                     RVAAKLGVSLVTDVTGLAIDGGAIVATRPVYAGKVIQKVKLKSVPALLSIRPNTFEPE
                     QSSANAANAPAESVPVPSFEARVKVTGIKAPDAAMLDVTEAPIVVSGGRGLKEPSNFK
                     LLEDLAEALGNTAVGASRAVVDAGWREHGAQVGQTGKTVSPGLYIAVGISGAIQHLAG
                     MRTAKTIVAINRDKDAPIFKVADYGIVGDLFEVVPRLTEEIRKMRSEQ"
     gene            complement(2938..4191)
                     /locus_tag="VFB89_07000"
     CDS             complement(2938..4191)
                     /locus_tag="VFB89_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZM27085.1"
                     /translation="MTSPRIPGFRGVPFTGVIYVMAEAARHGFRHGHPDWCNLGQGMP
                     ETGSLPGAPPRVHQVEISPDDQEYAPVPGIWELRRAVAELYNHLYRQDRASKYTERNV
                     CICGGGRASLTRAVAALGHINLGHFLPDYTAYEELLDIFKLFTAIPILLDPANGYHFS
                     LDELRREITGRGLSALLLSNPCNPTGKVVQGAELEAWVDTARELQCSLLIDEFYSHYI
                     WSGGDGDMVSAARYVQDVESDPVVLFDGFTKNWRYPGWRCTWIVGPQQVIDAVSSSGS
                     FLDGGGSKPLQRSAVPLLSPSVACGETAAIHRTFGRKREVLLEGLRRAGARFDLEPQG
                     TFYCWADVSGFPQGLSDGMALFRALLDQKVICVPGEFFDVNPGKRRSGRPSRFKNYVR
                     FSFGAEEEQVVEGVRRVEELVREKA"
     gene            complement(4310..5068)
                     /locus_tag="VFB89_07005"
     CDS             complement(4310..5068)
                     /locus_tag="VFB89_07005"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HZM27086.1"
                     /translation="MFWLRSLGGLEVECEARSLGGAAARPRPLALLALIASGRGRGMG
                     RERLLAYLWPERDTEHARNCLKQTLFALRQDVHDELFVGRAGLLRLNPAIITTDVLQF
                     EAAWARGAHHAAVALYRGPFLEGFHLSHVQEFDQWVESERLRLALYYRGCLEALGTAA
                     YEAGDCSAAVEWWRKLTTLAPLNSRFALGLMRSLVGLGNPAEALIHARVYERLVHTEL
                     GTPPDTTVSAYADWLRQYPEASLRRRSRSRSPEA"
     gene            complement(5697..>5992)
                     /locus_tag="VFB89_07010"
     CDS             complement(5697..>5992)
                     /locus_tag="VFB89_07010"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HZM27087.1"
                     /translation="GTAAYEAGDCSAAVEWWRKLATLDPLSSRFALGLMRSLVGLGDR
                     ADALIHARVYERLVRMELGTPPDTAVTTYADWLRQHPDATFRRWSRRRPASGA"
BASE COUNT         1129 a         1895 c         1983 g          985 t
ORIGIN      
        1 ctacgccaac ttcggcgcgt tcaatttcaa tgagacagtg cctggcgctc cgctcgagcg
       61 gttcctgact cccggacaaa agctggctct caggcttgtg attcgccgct cgtttgttgg
      121 ggcgcacgct ggggtcggca cggacaagga ctgaccaccc cgagtcagct cagcactcga
      181 catcaggcga cgaagtccag cagctggcag tagctgaggg tcagcgcctc gatcagctct
      241 tcatcggcga cacgaaggtc cggcggccgc gcatcgtcga acagaatcgc ctccttggcc
      301 gccgcatcca ccagcaggaa tcctagcaat actccccgct cgggatcggg atgagccatt
      361 gcttcccgat gctgcaacag gagcacggtg agccgccgga cgaactcagc gttgaaggcc
      421 gctgcctccg cctggaagga agcatcggga tgactccgaa cgaacaaatg cagcgctcgc
      481 agcaggcggc gcttcgcgcg atagctcctg accaacgctt tcaccactgc agccactagc
      541 tcccgggggg gcagccggcg ccagcgaggg tcgtccagca gcgcatcagc ggcacggcga
      601 gcccggtcga agaaggcaat ggtaaaggcg cggagtaacg cgtccttgcc ggggaaccgg
      661 gtgtagagcg tgccgacagc gatgtccgcg cgccgggcga cttcctggac cgtgagactt
      721 gccacgccgc gctcggcgat ggtgctctcg acggcgctca gcagtcggcc caaagcggct
      781 cggctccggg cttgttgggg agggggtagg ggcaactgcg cttgtctatg aatttgaatt
      841 cagattcatg ttacacagcg agaggcaggg gcgcaagcct gcctaccggg ccgccacgtg
      901 gtccgctacc tttgccccgc tttcaaccgc cgactgcccc tcgtgcccgt ccccgggtgg
      961 tcccgattat tgctcctcac ctgaggatgt tcgttttgaa gatcgccgtc tgcctcaagc
     1021 gggtcccgga tactgtcgcc aaaatcacga tcggcgccga ccggaagtcg atcgacgagt
     1081 ccggcctcaa gttcgtcccc aatccatacg acgagtttgc catcgaagaa gccattgccc
     1141 tgaaggaaaa ggcagggagc ggtgagacgg tggtctacgg catcgggacg gatgcggcgc
     1201 aagagaccct gcgagccgcc ctggcgatgg gaattgatcg cgctgttctg ctcaagagtc
     1261 ccggttcccc ggacggacta gagattgccc gggtgctggc cgcggaggtc aaagaaggcg
     1321 gctacgacct gatcctgtgc ggcaagcttg cggtggatga ctacaaccat caggtcggcg
     1381 tgatgctggc ggagctgctg gggctaccct gcatctcgtc ggttgctcac ctcacggtcg
     1441 cggatggatc cgtggaggcc gagcgggaga tcgagggcgg cgtcgaggta gcgacgtgcc
     1501 agctccccgc ggtgctgacc tgcgagaagg gactgaacaa tccgcgactg ccctcgctca
     1561 agggcatcat ggctgccaag aagaagccgc tggaaaccaa ggccgtcccg gtgggagtcg
     1621 gatcgatcga ggtactgtca ctcgactatc ccgccgaacg aaagccgggg aaagtggtag
     1681 gcgaaggggc cgatgctgtg cccgagctgc tccgcctgct ccggacggaa gcgagggtgc
     1741 tctgacatgc ctgccaacgc gatcctcgca gtactggaga ctcgtgatgg agcgctcagg
     1801 aaggtctccc acgaggttgt agccgccgcg cggcagattt ctaccgcggg aggatccgtc
     1861 gacgcgcttg ccttcggtgt ctctgctcct gaaggaagca atggcgtcgg ggcgaaccgg
     1921 gtgctggtgg ccgctcatgc cgagttcggc cggtacaacc ctgatggctt tgctgcgacg
     1981 gtggcgtcgc ttgcggccgg ctacggcgcc atcgtattcg ccgcgaccgc gaccggcaga
     2041 gatctcgccc cccgggtcgc tgcgaagctg ggtgtgagtc tggtgaccga cgtaaccgga
     2101 ctcgcaatcg atgggggtgc catcgtcgcc acccgcccgg tctacgctgg caaagtgatc
     2161 cagaaggtga agctcaagag cgtcccggca cttctctcca tacggcccaa caccttcgag
     2221 cctgagcaga gctcggccaa tgctgcgaac gctcctgcgg aatcggttcc ggttccttca
     2281 ttcgaggccc gggtgaaggt cacgggaatc aaggccccgg acgcagcgat gctcgacgtg
     2341 acggaagctc cgatcgttgt atccggtggc cgaggactga aggagccgag caatttcaag
     2401 ctgctcgagg atcttgcaga agcgttgggc aacacggcgg tgggcgcgag ccgggccgtg
     2461 gtcgatgccg gctggcggga gcatggtgcg caggtggggc agactggaaa aaccgtgagt
     2521 ccgggtctgt acattgcggt cggcatctcc ggcgcaattc agcatctggc cggcatgcgc
     2581 acggcgaaga cgatcgtcgc catcaatcgc gacaaggacg caccgatctt caaggtggcg
     2641 gactacggga tcgtaggcga tctgttcgag gtggtgccta gactgacgga agagattcgg
     2701 aaaatgagga gcgagcagta gcgcaaagcg tgaacagcaa gccgacggtg cagtggccac
     2761 gccttgggcg ctgcggttcg ctatggttgg aagacggcct cgcggggacc cggggggtgc
     2821 tggggttggc ggggcttggc cagcgccccc gggttcccgc gagcccgtct tcttcgatcc
     2881 cgctcaccga cgcgcccgtc ggcgtggcca ccgcaccgtc ggctcactgt tcaccgttca
     2941 cgctttttct ctaaccagct cctctactcg cctgaccccc tctaccacct gctcctcctc
     3001 cgcaccgaag gagaacctca cgtagttctt gaagcgggac gggcggccgc tgcggcgctt
     3061 gccgggattg acgtcgaaga actcgcccgg tacgcagatc accttttggt cgaggagagc
     3121 gcggaacagg gccatgccgt cgctcaagcc ttggggaaat cctgacacgt cggcccagca
     3181 gtaaaacgtg ccctgcggct ccaggtcgaa acgggcaccc gcacggcgca acccctccag
     3241 caagacctct cgcttccggc caaacgtgcg gtggatcgca gccgtctctc cacaggccac
     3301 actgggcgac agcaacggta cggcggatcg ctggagcggc ttgctgccgc cgccatcaag
     3361 gaacgatccg ctactcgata cggcgtcgat cacctgctga gggccgacga tccaggtgca
     3421 gcgccagccg ggatagcgcc agttcttggt aaagccgtcg aacagaacaa ccgggtccga
     3481 ctccacgtcc tggacatacc gggctgcgga caccatgtcg ccgtcgccgc cggaccagat
     3541 gtagtgggaa tagaactcgt cgatcaggag tgagcactga agctctcgcg ccgtatcaac
     3601 ccacgcctcg agctcggcgc cctggaccac cttgccggtc ggattgcagg gattggaaag
     3661 caggagcgcc gacagccctc gtccggtgat ctcgcgccgg agctcgtcca ggctgaagtg
     3721 atagccgttc gctggatcca gcaggatcgg gatcgcggtg aacagcttga agatgtcgag
     3781 cagctcctcg taggcagtgt agtcgggcag aaagtgcccg agattgatgt ggccaagcgc
     3841 agcaaccgcg cgggtgagtg aggcgcggcc acccccgcaa atgcacacgt tccgttccgt
     3901 gtacttggac gcgcgatcct gccggtagag gtggttgtag agttcggcga cggcgcggcg
     3961 aagctcccag attcccggca ccggagcgta ttcctggtcg tccggactga tctccacctg
     4021 gtgcaccctg ggcggcgcgc ccggaagcga tccggtctcc ggcataccct gacccaggtt
     4081 gcaccagtca ggatggccgt gacgaaagcc gtgtcgcgcg gcctcggcca tcacgtagat
     4141 gacaccggtg aagggaacgc cgcggaagcc ggggatgcgt ggggaggtca tctacgaagg
     4201 taactgcgaa aggctgaacg acgaacggga cctggccggc gacgttactc ggctcccgtg
     4261 ggtgaacgcg attcaggaaa atcgcttccg ttggcggcac tagtctgctt caagcctccg
     4321 gggacctgct gcgagaccgc cggcgcaagc tggcctccgg atactggcgc agccagtcgg
     4381 catacgccga cacagtcgtg tcaggcggtg tcccgagctc ggtgtggacg aggcgctcgt
     4441 acacgcgggc gtggatcaag gcttcggccg ggttccccaa accgaccagc gaccgcatga
     4501 gacctaacgc gaaccgtgag ttcagcgggg ccagggtcgt cagcttgcgc caccactcga
     4561 ccgcagcgct acaatcgccg gcctcgtacg cggcggtgcc cagagcctcg aggcacccgc
     4621 gatagtagag cgccaggcgg aggcgctcgc tttccaccca ctgatcgaat tcctgaacgt
     4681 gggaaagatg gaagccctcg aggaagggtc cccggtagag ggcgacggca gcgtgatggg
     4741 cgccgcgcgc ccacgccgcc tcgaactgga gcacgtcggt cgtgatgata gcgggattga
     4801 gtcggaggag tcctgcacgc cctacgaaga gctcatcatg gacatcctgt cgcagcgcga
     4861 acagcgtctg cttgagacag ttccgggcgt gttcggtgtc ccgctccggc cagagataag
     4921 cgagcagccg ctcgcggccc atgccgcgcc cgcgccctga cgcgatcagc gcaagcagcg
     4981 cgagcggtcg cggtcgcgcc gcagcaccgc cgagcgaacg cgcttcgcac tcgacctcga
     5041 ggcccccgag acttcgcagc cagaacatgg ctcaacctgc ttgaccggcc gaaaacttgt
     5101 ccgtggcgcg cgccacgggc gtggcgcaag aagcggggta ccagagggat aagcgacata
     5161 agtcggaagc gtctcatgcc ctggtttagc tcacggagaa gccaaaagcg ccacatggat
     5221 tccatcaggc aggacctccg cccactagac ataaccaact tctaaccggg aattaactct
     5281 cgccgagagc ggctgtcatg cgtttctcgg agaacacgcc gccgcgaacg gggtctcatt
     5341 ggtcagtcat tcaaccatgt agcgccgcgc ccaaaaccag cgcgctcggc tagagatgtc
     5401 gactccctca cggtgttacc tcggcactac caaaacagaa ccccaagcag ttcctgccgt
     5461 gagaagactc gagcaggacc tattcggcat cgctggggtg tgacgtgcga gaaccggctc
     5521 tcaccggatt cgcgtcagcc gagcctcagc agtcgcggcc tctcgagaga gagggcgggc
     5581 gctggggagc catcaggcgg agcgagcggg cgtgatgtgg cctacgatca gcggaagccg
     5641 ggtcgcgagg ttgatcggat cgtgcggttg aggagattgg cgtcctgtca ttggcctcag
     5701 gccccggacg ccggcctcct gcgagaccac cggcggaaag tggcgtccgg atgctggcgc
     5761 agccagtcgg cataggtcgt cacagccgtg tccggcggtg tcccgagctc catgcggacg
     5821 aggcgctcgt acacgcgggc gtggatcaag gcgtcagccc ggtcccccaa accgaccagc
     5881 gaccgcatga ggcctaacgc gaaccgcgag ctcagcgggt ccagggtcgc cagcttgcgc
     5941 caccactcga ccgcagcact acaatcgccg gcctcgtacg cggcggtgcc ca
//
LOCUS       DASRJQ010000314         9880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3476990, whole genome shotgun sequence.
ACCESSION   DASRJQ010000314 DASRJQ010000000
VERSION     DASRJQ010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 9880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9880
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3476990"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2129)
                     /locus_tag="VFB89_07015"
     CDS             complement(<1..2129)
                     /locus_tag="VFB89_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM27088.1"
                     /translation="MALTPTGLADALQDRYVLERELGRGGMAIVFLARDLRHQRPVAL
                     KVLDSDVLSTVGAERFQREIHVAAGLQHPHILTVLDSGEAGGRQWFTMPFVDGESLRD
                     RMRRDGPLPVDEALRLAREVADGLEHAHRHGVIHRDIKPENILLSAGHALIADFGIAR
                     AVAEPTSDPGSALPGPLTRTGLVVGTPDYMSPEQASGERTLGPSSDVYSLAIVLYEML
                     AGSPPFTAPTARAAMAKHFSGDMPSLREARPEVPEAIEAAIRKALAPEPEDRFASAAR
                     FAEALDRDRRTGDTVVTTGTSASAAQAAPLGRSRLAARLLVPIGLVAALGIGLGLWWT
                     RPGAHAGSTAVSRLAVLPFENVGDAEDEYFADGLADAVRGKLSAVPGLQVIASSSSDQ
                     YKKSGKSPQEIGRELGAEYLLIGKVRWQKTGGGVSRVQVSPELIQASSATTRWQQPFD
                     APLQDVFRVQVDIATRVAQALDVALGAGARQQLTERPTRNLAAYDAFLRGEQLSNRVG
                     ITDLDALRRSVVAYSEAVALDSGFALAWAQLSRALSTIYVNGSRPETEAQRAADAAQR
                     ALALGPDLPQAHFAQALYLSGVRHEHARALEEVTLARKSAPYDVELLSMAALAEQQLG
                     RWDDALMHFREAQSLDPRSVGTARRLARVLLWLRRYPEARQACDDALRLSNASPDVLD
                     FKVATFLGEGDLAGARAALRDAISGLDT"
     gene            2269..2685
                     /locus_tag="VFB89_07020"
     CDS             2269..2685
                     /locus_tag="VFB89_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27089.1"
                     /translation="MPDRVRKVNYCYPVVPNRAGQGARVLAEIKRAGINLLAYSGFPI
                     GGGKSQLDLVLDDIGPLRRLARRNGWRLSAVKKGFLIQGSDEVGAVHRHLDKLGNAGI
                     NVTAADAVSAGKGRYGMLLWVRPRDYARAAKALGAR"
     gene            complement(2682..3653)
                     /locus_tag="VFB89_07025"
     CDS             complement(2682..3653)
                     /locus_tag="VFB89_07025"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZM27090.1"
                     /translation="MLLPSRPAYLSLGSAELHRRAERAIATLADCRACPRDCRVNRLE
                     DKWAACKTGRYAIVSSHFPHFGEEDCLRGWRGSGTIFFGHCNLRCVFCQNYDISQAIR
                     PGTTTPGHRPEQIAAMMLELQDRGCHNINFVTPEHVAPQVIEAVAVAADRGLTLPIVY
                     NTSAYDSLESIELMDGIVDIYMPDLKYWSAERSRSYLKAENYPEAARAAIKAMHRQVG
                     QLVVDGEGLARRGLIIRHLVMPGTLDETRAILQWIVDELGRDTYVNLMDQYYPAGKVD
                     GEHYPEINRRLTSQEFAQGRAMAQQLGLRRLDARRPHPRLARRLVGF"
     gene            3916..4320
                     /locus_tag="VFB89_07030"
     CDS             3916..4320
                     /locus_tag="VFB89_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27091.1"
                     /translation="MEARVTSYSQAERLRERMEKASVRHGEEIRADLPGIRVAASPRN
                     WFVSRTKHKGEVFFCTMGPLEVREVVSPGDDEPLPTTAVVEGLMVPSSGTYDLFNALV
                     QSNGDLRVIVDQATRVVPSAGRGIFAREEAIW"
     gene            5018..6286
                     /locus_tag="VFB89_07035"
     CDS             5018..6286
                     /locus_tag="VFB89_07035"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZM27092.1"
                     /translation="MTRRLLPFLLLLYIVCFLDRVNVGFAALRMNQDLAFSPAVYGFG
                     AGVFYLGYVVFEVPSNLILARMGARVWIARIMITWGLLAAAMMFVQGPLSFYTLRFLL
                     GVAEAGFFPGIIYYMSGWFPAAQRARATARFMIAIPLSSVVGGPLSGALLGLNGSFGL
                     AGWQWLFLLEGLPAVVLGFVVLGYLTDRPENARWLAPAERAWLSTHMSQEREHCARHY
                     GSSARQALSNHTVWQLGLLTFLCTGFGQQVLGLWLPQIVSSFSNLSDFLVGIVSAVPY
                     LIAAVAMVLVGAHSDRSGERILYVAGCAIIAAVGFLASAYLHSPALSVLALSVAAAGL
                     LSAHGPFWALPPTFLSGRGAASGIALICSIANLSGFVGPYAIGLLKGASGSFHSGLLL
                     LAVVPLVGAGLALHIRRASALSPSLTTAYP"
     gene            complement(6311..6460)
                     /locus_tag="VFB89_07040"
     CDS             complement(6311..6460)
                     /locus_tag="VFB89_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27093.1"
                     /translation="MGSARAQGSVFPRRQRGRLAEAVDHYAQLFDLSLTKQQKADLVE
                     YLKTL"
     gene            6683..7333
                     /locus_tag="VFB89_07045"
     CDS             6683..7333
                     /locus_tag="VFB89_07045"
                     /inference="COORDINATES: protein motif:HMM:NF016581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HZM27094.1"
                     /translation="MRVPHWNRLMESSAGQVMRLLRRGPMTVDELAVALDVSGNAVRP
                     QLAILERDGLVQRSDLRRGTSKPARTYVLTAEAELLFSRAYVPVLTQLLQVLDQRLEP
                     REFNRIMREVGRQLMGDRTRQTGGLRQRAEAASALLNELGGLTEVEPGRTGFRIRGYG
                     CPLAAATQRHPEACNAVESLLTEFAGVPVSKCCEGEDRLRCCFEIGATRPSARARA"
     gene            complement(7305..8408)
                     /locus_tag="VFB89_07050"
     CDS             complement(7305..8408)
                     /locus_tag="VFB89_07050"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HZM27095.1"
                     /translation="MTRKTDTTPFVQANDAYERWLGAHLRLVRVDLHRKHAAMAEGAF
                     QFLRATFFRWAQRWPVVCPDLAEAPKVLAVGDLHVENFGTWRDAEGRLVWGVNDFDEA
                     WALPYTADLVRLATSAMVAASEHELSLDERQVCEVVLEGYGRGLEGGGRSFVLAEHHP
                     ALRAMAVERLKNPERFWEKLERLPPIRGTVPVGARKALKRMLPDPEMEIRYVHRIAGL
                     GSLGRERVVAMGDWHGGTVAREAKAAAPSACAWAAGIQAEDAKVHYQTIIERAVRCPD
                     PFLRLKRRWIVRRLAPDCARIELSALPRQRDEKRLLGAMGWETANVHLGSERARTLQA
                     DLKTRGKAWLLHAARRMMEEVESDFKPWRAHWA"
     gene            complement(9204..>9880)
                     /locus_tag="VFB89_07055"
     CDS             complement(9204..>9880)
                     /locus_tag="VFB89_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27096.1"
                     /translation="GRAVDQALGERMRQGGLFRLANLINNQPQLNLEGSARLRGGLVG
                     PDEYSVTGRFELGFVNVNSYQGFCRQRQQAASLDCLEAYLRRSGVRQSLDHGDRLWLA
                     ATFTSHSDFVAVVPDQGINFALAGSSSFDVNAGLGRYVALNSQGVETGRIDASLEFQA
                     NDASTMRNDRLIGSLSYTHRVTDTVSLLVGLVYANKPEFITDVDRKVSGNIGLRYRLQ
                     PPLPAE"
BASE COUNT         1675 a         3240 c         3227 g         1738 t
ORIGIN      
        1 gtgtcgagcc cgctgatggc gtcgcgcaga gctgcgcgtg cccctgcgag atcgccctcg
       61 ccaagaaacg ttgccacctt gaagtcgagc acgtcgggcg acgcattgct caggcgcagg
      121 gcgtcgtcgc atgcctgcct cgcttccggg tagcgccgga gccatagcag cacgcgcgca
      181 agccgccgcg ccgtccccac cgagcgcggg tccagcgact gggcttcccg aaaatgcatc
      241 agcgcgtcgt cccagcgacc caactgctgc tccgcgagcg ccgccatcga cagaagctcc
      301 acgtcgtacg gagcgctctt ccgtgccagg gtcacctcct cgagcgcgcg ggcatgctcg
      361 tgacgcacgc cgctcagata cagggcctgg gcgaagtgtg cctgcggtaa gtcggggccc
      421 agcgcgaggg cccgctgagc tgcgtcggcg gcgcgctgtg cctcggtttc gggccggctt
      481 ccattgacgt agatggtcga cagcgcccgg gagagctggg cccaggcgag cgcgaatccc
      541 gaatcgagcg ccacggcctc gctgtaggcc actaccgagc gccggagcgc gtctaggtcg
      601 gtgatgccca ctcgattgga gagctgctca ccccgaagga acgcatcgta ggccgcgaga
      661 ttccgggtcg gccgctcggt cagctgctga cgcgcacccg cgccaagcgc cacatcgagc
      721 gcttgcgcca ctcgagtcgc gatgtccacc tggacccgga agacatcctg cagaggcgcg
      781 tcgaacggct gctgccaccg ggtggtcgcg ctcgatgcct gaatcagctc cggactcacc
      841 tgaacccggc tcacgcctcc acccgtcttc tgccagcgca ccttgccgat caggagatac
      901 tcggcgccga gctcacgccc gatctcctgc ggactcttgc cgctcttctt gtattgatct
      961 gagctgctcg acgcgatgac ctgaaggcct ggaaccgcgg agagcttgcc gcgtaccgcg
     1021 tcggcgagcc catcggcgaa gtactcgtcc tccgcgtcgc ccacgttctc gaacggaagc
     1081 acggccaggc gggataccgc cgtgctgccc gcatgggcgc ccggccgagt ccaccatagc
     1141 ccgagcccta tacccagagc agccacgagc ccgattggga cgagaaggcg cgccgctagc
     1201 cgcgaacggc caagcggggc ggcttgcgct gccgacgccg aggtgcccgt ggtgacgact
     1261 gtgtcaccgg tccgccggtc ccggtccaat gcctccgcga acctggcggc gctcgcgaaa
     1321 cggtcctccg gctccggtgc cagcgctttc cggattgcgg cttcaatcgc ctccggcact
     1381 tcaggccggg cttcgcgcag gctgggcatg tcacccgaga aatgcttcgc catggcggcc
     1441 cgcgccgttg gagccgtgaa gggcggggag ccggccagca tctcgtacag cacaatggcc
     1501 agggagtaca cgtcggaaga tgggcccagt gtgcgctcgc cgctggcctg ctcggggctc
     1561 atgtagtccg gcgttccgac gacaagaccc gtccgcgtga gcggacctgg cagtgcgctg
     1621 ccaggatctg acgttggttc cgccactgcc cgtgcgatgc cgaagtcggc gatgagggcg
     1681 tgtcccgcgg agagcagaat gttttccggc ttgatgtcgc gatggattac gccatggcgg
     1741 tgggcgtgct cgagcccgtc ggcgacctcg cgcgctagac ggagggcttc gtccacgggg
     1801 agcggtccgt cgcggcgcat ccggtcgcga aggctctcgc cgtcaacgaa cggcatggtg
     1861 aaccactgcc ggccgccagc ctcacccgag tcgagaacag tcagaatgtg gggatgctgg
     1921 agcccggcag cgacgtggat ctcacgctgg aaccgttcgg cgccgacggt cgaaagcacg
     1981 tcggagtcga gcaccttgag ggcgacaggc cgctggtgcc ggaggtcgcg tgccaggaac
     2041 acgatcgcca tgcctccgcg ccccagctcg cgctcgagca cgtatcgatc ttgcagggcg
     2101 tcggccagcc cggtcggggt aagggccaca gcggcggtgc ctgtcggtga cgttaggcga
     2161 aactagcggc ccggaggttg acgggcaccc ccttccggcc cgcggtacaa tcccgcacta
     2221 tgccccattg cacaccagac tttactcaac ctccaccagg aggcgctcat gccggatcga
     2281 gtgagaaagg tcaactactg ctatcccgtc gttcctaacc gtgcgggcca gggcgcgcgt
     2341 gttctcgccg agatcaaacg cgccggtatc aacctccttg cctactccgg tttcccgatc
     2401 ggcggcggca agtcccagct cgatctcgtg ctcgacgaca tcggcccgct ccgccggctg
     2461 gcccgccgga acggctggcg actgagcgcg gtaaagaagg ggttcctgat tcagggaagc
     2521 gatgaagtgg gcgccgtgca tcgccacctc gacaagctgg gcaacgccgg aatcaatgtc
     2581 acggctgcgg acgccgtgtc agcgggcaag ggacgctacg gcatgcttct ctgggtcagg
     2641 ccacgcgact acgcgcgggc ggccaaggcg ctgggggcgc gctagaaacc caccaggcga
     2701 cgggcgagcc ggggatgggg ccgtcgcgca tccagccggc gaagccctaa ttgctgggcc
     2761 atggcccggc cttgggcgaa ctcctgggag gtgagccgcc ggttgatctc cgggtagtgc
     2821 tcgccgtcca ccttcccggc cggatagtac tgatccatca ggttcacata cgtgtcgcgg
     2881 cccagctcat cgacgatcca ctggagaatc gcgcgcgtct cgtccagcgt tcccggcatc
     2941 accaggtggc gaatgatgag cccgcgccga gccagccctt cgccgtcgac gacgagctgc
     3001 cccacctgcc ggtgcatcgc cttgattgca gcgcgcgccg cctcgggata gttctccgcc
     3061 ttgaggtagc tgcggctccg ctcggccgac cagtatttca ggtccggcat gtagatgtcc
     3121 acgatcccat ccatcagctc gatgctctcg agcgaatcgt aggcactggt gttgtaaacg
     3181 atcggcaggg tgagcccgcg atcggccgca acggcgacgg cctcgatgac ctggggggcc
     3241 acgtgttccg gtgtgacgaa gttgatgttg tggcagcccc ggtcctggag ctcgagcatc
     3301 atggcggcga tctgttccgg gcgatgaccc ggtgtggtcg ttccgggacg aatcgcctgg
     3361 ctgatgtcgt agttctgaca aaagacacag cgcagattgc aatggccgaa gaagatcgtt
     3421 cccgatccgc gccacccccg aagacagtcc tcctcgccga agtggggaaa atggctcgag
     3481 acgatcgcgt agcgtcctgt cttacatgcg gcccacttgt cttccagccg atttacccgg
     3541 cagtcccggg gacaggcacg gcagtcggcg agggtggcga tggcccgctc ggcgcgccga
     3601 tgcagctcgg cgctgcccag ggagaggtag gcggggcggg acggtagcaa catggagcac
     3661 gcgggtcgaa cgatcgaatc tcctcaaaga tagtgtgcga tacgttcttg acgcgccgcg
     3721 ggccgtaccc tagcgtgaga atgccagcat gattcggcac agccggcctg aggccggcgc
     3781 cgcacgccag gctcctagtt cctccgcctc gccacccact cgacgtcacg aaggactagc
     3841 aggatagctg cgctgcccca gctggcatgc gcgtcgagcg cgttcacagg ttttccactg
     3901 cagtagaggt gagaaatgga agcacgggtc acgagctact cccaggccga acggctcagg
     3961 gagcgcatgg aaaaagcctc tgtacggcat ggggaggaaa tccgggcgga ccttccgggc
     4021 atcagggtgg ccgccagccc ccgcaactgg tttgtcagtc ggaccaagca taagggcgaa
     4081 gtctttttct gcacgatggg accgctcgaa gtgcgagagg tagtgagccc gggggacgac
     4141 gagccactgc ccaccacggc cgtggtcgag ggattgatgg tgccgagcag cggcacctat
     4201 gacctgttca acgcactggt tcaatcgaac ggagatctgc gcgtgatcgt ggatcaggcg
     4261 acccgggtcg tgccatcggc agggcggggg atcttcgccc gagaggaagc gatctggtga
     4321 agcaaaagag acggcccgca ctctcttgag agagtagcgg gccgtcagcg caccggttac
     4381 ccggagtcgg gcaattgact tgcccgttct tcttcccgca gctaccgcct aactgatgcg
     4441 tttgcggtcc agccttggga gctccggctt ccatccccgg acggttcgcg ggccgtccgg
     4501 ttcaccgatg cgagcgctgc aagtccggct gaatcgcgga tcaacctctt gcacggcatg
     4561 ccgcggcgcc gctgcggcat gggcaaggcg cgcgattggc ctcgccgatt tggctcgaga
     4621 gcgatcaaac ctcaagccag aaaatgagaa atcacatact acgattcggt acattgcgcc
     4681 gcaagcaccg gagcggcagc gcatgctggg caatcgagtt cttattcgcc ccgccgcagg
     4741 cggcgaaggc agatgccttt ttcacaggct cgcgatctgc tgccgttctg agttgccgcc
     4801 gtcctactac ggagtgcggg cggtctgacg gctgccggtg ccgtagtccc cttgcgcttg
     4861 agtcgcgctc attttgcacg gtttaagact ttcttgcctg gcgccggccg atgggtatgt
     4921 tcgatctgcc ttcccccagg ccgagatgtc tgccgtgcta ctgcgctcaa tggggcctcc
     4981 ccacgctcgc acattagagc agatcaccct aacaagggtg acccggcggc tgctgccgtt
     5041 tcttctcctc ctctacatcg tatgcttcct ggaccgggtg aacgtcggat tcgctgctct
     5101 ccggatgaac caggacctcg cgttcagccc cgcggtctac ggtttcggcg ccggcgtctt
     5161 ctatctgggc tacgtcgtgt tcgaggtgcc gagcaacctc attctggcgc gaatgggcgc
     5221 ccgcgtgtgg atcgcgcgga tcatgattac gtgggggctg ctggccgctg ccatgatgtt
     5281 cgtgcagggc cccctgagct tctacacgct ccgctttctg ctcggcgtgg ccgaggcggg
     5341 cttcttcccc ggcatcatct actatatgag cggatggttt ccggccgcgc agcgggcccg
     5401 agcgaccgcg cggttcatga tcgccatccc gctctccagc gttgtcggcg gtcccctttc
     5461 cggcgcattg ctggggctca acggctcctt cgggctcgcc ggctggcagt ggctctttct
     5521 cctcgagggt ctgccagctg tggtgctcgg cttcgtcgtc ctgggctact tgaccgaccg
     5581 acccgagaac gcacggtggc tcgctcccgc ggagcgcgcg tggctcagca cgcacatgag
     5641 ccaggaacgt gagcactgtg cgcggcacta tggttcgagc gcgcgtcagg cgctctcgaa
     5701 ccacaccgta tggcagcttg gactgctcac ttttctctgc acagggtttg ggcagcaagt
     5761 cctcggcctc tggctccccc agatcgtcag cagcttctct aacctgagcg actttctggt
     5821 cggtatcgtc tcggcggtac catacttgat tgcagccgtg gccatggtgc tcgtcggtgc
     5881 acactcggac cgaagcgggg agcgcatcct ctacgttgcg gggtgcgcta taattgcggc
     5941 ggtcggcttc ctggcgagcg cctacttgca ttcacccgcg cttagcgtgc tcgcgctatc
     6001 ggtcgcggcg gccggcctgc taagcgctca tggacccttc tgggccctcc cgcccacctt
     6061 cctgagcggc cgcggcgccg ccagtggcat tgccctgatc tgctctatcg caaacctgag
     6121 cggctttgtg ggcccctacg ccatcggcct gctcaagggc gccagcggaa gcttccacag
     6181 cgggctcctg ctgctcgcgg tggtgccgct ggtcggcgcg gggctagcgc ttcacatccg
     6241 ccgtgctagt gcactctccc cctcgttgac gaccgcctat ccgtagcgtc gtagttacct
     6301 atcgcacggc ctacaaggtc ttgagatatt cgaccaggtc cgccttctgc tgcttggtga
     6361 gcgagaggtc gaaaagctgg gcgtagtgat cgaccgcctc ggcgagcctg cctcgttgcc
     6421 gtcgtggaaa tacggaccct tgagctctgg cggatcccac aggccacgga gcggccgacg
     6481 attcgcaatg cgctcactcg agcgcctgac atgcggcgga ccatcgaaga cctgaaccga
     6541 ctaactttgt tcgaccgagg ctgtcccatt gcgaaacagc cggagacaca gaaagttaag
     6601 aagttgcttg acaatcttta gacgtcccga atcgtgcagt gacagctctc acggccgaaa
     6661 gggcaaggag tgaaagagcg cgatgcgtgt accacactgg aaccggctga tggagagctc
     6721 ggccggccaa gtcatgagac tgcttcgacg cggccccatg actgtggacg agctggccgt
     6781 ggcactcgac gtcagcggca atgcggtgag gcctcagctc gcgatactcg agcgggacgg
     6841 gctggtgcaa cgcagcgacc tgcggcgggg cacgagcaag cctgcgcgaa cctatgtgct
     6901 cacggccgag gccgagctgc tattctcccg cgcctacgtc ccggtcctga cccagctgct
     6961 gcaggtgctc gaccagcgac tcgagccccg cgagttcaac cggatcatgc gcgaggtagg
     7021 gcggcagctc atgggagatc ggacacggca aacgggaggc cttcggcagc gggccgaagc
     7081 ggcgagtgca ctgctcaatg agcttggcgg actgactgag gtcgagccgg ggcggaccgg
     7141 ttttcggatt cggggttatg gctgcccgct cgcagcagcc acgcagcggc atcccgaggc
     7201 ctgcaatgcg gtagaaagcc tgctcacgga attcgctgga gtcccggtgt ccaagtgctg
     7261 cgagggtgag gatcgcctgc gatgctgctt cgagattgga gctactaggc ccagtgcgcg
     7321 cgccagggct taaagtcgga ctctacctcc tccatcatcc gccgagcggc gtgtagcagc
     7381 cacgccttgc cgcgagtctt gagatccgcc tggagggtcc tcgcgcgctc gctgccgagg
     7441 tgaacgttcg cggtttccca gcccattgct cccagcaggc gcttctcgtc ccgttggcga
     7501 ggtaatgcag agagctcgat ccgggcgcag tcgggcgcga gtcgccggac gatccagcgg
     7561 cgcttgagcc ggagaaaggg atcggggcag cgcaccgctc gctctataat cgtctgatag
     7621 tggaccttgg catcctcggc ttggatgccc gccgcccagg cacacgccga tggagcggct
     7681 gctttggctt cccgtgcgac cgtcccaccg tgccagtccc ccatcgcgac cacccgctcg
     7741 cgacccaggc tgccgagacc cgcaatgcga tggacatacc ggatttccat ctccggatca
     7801 ggcaacatgc gcttgagcgc cttgcgagcc ccaacgggga cagtcccgcg gatcggcggc
     7861 agcctctcca gcttctccca gaaccgttcg gggtttttca gacgctcgac tgccattgca
     7921 cggagcgcag ggtggtgctc cgccaggacg aacgatctgc cgcctccctc gaggcccctg
     7981 ccgtaaccct cgagcaccac ctcgcagacc tgcctctcat ccaacgagag ctcgtgctca
     8041 ctcgctgcga ccatggcgct ggtcgcgaga cggaccagat cagcggtgta ggggagcgcc
     8101 caggcctcgt cgaagtcgtt cactccccag acaagtcgcc cttcggcgtc gcgccaggtg
     8161 ccgaagtttt cgacgtgcag gtctcccacc gccagcacct tgggcgcctc ggcgaggtcg
     8221 ggacagacca ccggccagcg ttgcgcccac cgaaaaaagg tcgcgcgcag gaactgaaac
     8281 gctccctcgg ccatggctgc gtgtttccga tggagatcca cccggacgag gcgcaggtgg
     8341 gcgcccagcc accgctcgta cgcgtcgttg gcctgaacga agggcgtcgt gtcagtcttg
     8401 cgggtcattc gtccagcgtc gcactatggt tgcgcgtact agggtcagtc cactccggta
     8461 cgtcgcggcg aagtctattg ttgaaccccg ttctccggcc cgaagccgag gtggccacgg
     8521 gcaatgtgac gcagatccta gtcggatggg acagcgattc ctatgctttt actgcgaacc
     8581 cgaaagggct tcgcactgag ccaagctgaa tcgactcgtc cgagttgaca ggcttggctc
     8641 ttttactgct gaaagccccg gccccgcgag ggccggcgta gtggctcagg aggttttatg
     8701 agtcgaagcg atttgacccg gtggtctttc atgctcgcgc tgctgtccac gcccgccgtg
     8761 cttcgagcgc aggctccggc cccggacgcc gccaacgcca gggccgacgc ggccaaggcc
     8821 gaggccgcca ggaaggccag ggaaaaagct gaagccgtga tctgcagaga cgggacctcc
     8881 ggccccaatg ctcaagcgtg caccggccat ggtggagtcg accccgtgac aaccagtgcg
     8941 tccagaaccg gccgggttga ccgtggcgca ggcggggccg gcggagcaga cggccgtgct
     9001 gccggtcgcg agggcgaggc agaagacacg atccggatca agcccgatac caaggaccct
     9061 gacaccggcc aaggcggcgg tggcataccg gacgggtggg gtggcggccg cgacggcgga
     9121 gacggatggg gcgacccatg gggcagcggg aacgacgcaa acggcaacgc cggcgataat
     9181 gacacgcctc ccgaggcaat gcttcactcg gcggggagcg gcggctggag cctgtatcgg
     9241 agacctatgt tgccggaaac ctttcggtcg acatcggtga tgaactcggg cttgttggcg
     9301 tagaccaggc cgacgagcag cgacaccgtg tcggtaacgc gatgggtgta gctgagggac
     9361 ccaatcaggc ggtcgttgcg catcgtgctg gcgtcgtttg cctgaaattc caagcttgca
     9421 tcaatacgtc ctgtttccac gccctggctg ttcagcgcca cgtaccggcc gaggcccgcg
     9481 ttgacgtcga atgacgacga tcctgccagg gcgaagttga taccctgatc cggcacgacc
     9541 gccacgaaat cactgtgcga cgtgaaagtg gccgcaagcc aaagacggtc accgtggtcc
     9601 agcgactggc ggacacccga ccggcgtaga tacgcctcca gacagtcgag gctcgcggct
     9661 tgctggcgct ggcggcaaaa accctggtag ctgttcacgt tgacaaagcc taactcgaaa
     9721 cgcccggtca cgctgtattc atcggggccg accaggccgc cccgcagccg tgctgatccc
     9781 tcgaggttga gctgtggttg gttgttgatc aggttcgcca gacggaacag gccgccttgc
     9841 ctcatgcgct cgccgagtgc ctgatccaca gcccgaccag
//
LOCUS       DASRJQ010000315         3101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3975495, whole genome shotgun sequence.
ACCESSION   DASRJQ010000315 DASRJQ010000000
VERSION     DASRJQ010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3975495"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="VFB89_07060"
     CDS             complement(<1..1008)
                     /locus_tag="VFB89_07060"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZM27097.1"
                     /translation="MIPLSLRALARRPLRAALAIGGVAVASAMLLDMVMLSGGIERSF
                     EQMLLGRGYQIRLSPKGTLPFDTEASVTRVSGLIERLERDPEIVGVAPVLGGSLYARS
                     ADSLLTVFGYGIDPAAQFLYQVEAGRDLGAEDTLGILLSRASARAIQAGPGDTIRIVG
                     RLDPQIGAAAVERSVVVRGLVRWLYDYQDQRSVGVVLPLMQRLLGPLATDRASLIVLK
                     VRADSAVPAVAARLRVEFPSVEVNSVAQLVVHFRERLVYFRQLSYILGAISLVVTVLL
                     VATLLTITVSERLGEIATLRAIGVRRSRIIRQMLGEGLALTGSGAVLGVLLGLATARY
                     LD"
     gene            complement(1005..1823)
                     /locus_tag="VFB89_07065"
     CDS             complement(1005..1823)
                     /locus_tag="VFB89_07065"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZM27098.1"
                     /translation="MGAPRCGAIQAPSPNTDSLIHIAVERRLARELGLRVGDTVRLGP
                     TVDSVSTPAVVAAVYEPRPDPSGIIRPDFHVRLHLPDLSRLLGAQDRVDRFGIGIQPG
                     ADPRRGAEKLNQVAFGFRAFESDEIASKSSQTFLVVSRFHRAIALITVVASAIFLLCI
                     MLLKVEERRNDVAVMRFVGVRRRVVFLAIVIEAALIALLGSAAGVAMALAAGAATNAY
                     YRRLFDTQLIFSQVTPRLLIFGVVLSLVLGLGAGAVAAWRLVRTPPLALWGRAG"
     gene            1949..2956
                     /locus_tag="VFB89_07070"
     CDS             1949..2956
                     /locus_tag="VFB89_07070"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HZM27099.1"
                     /translation="MADSDRPRVAVIGAGHRGRGWAALALSRGWPVSLYDLDSALLQR
                     ASADVAERVRRLTDLGQADPLAAAGALDSLRIGRSLLHAVGDAELVLDAMPLDLAGKQ
                     RLVEQIEQVSRAASITVSMTGTMASSALCARLRRPERHAVAYALDPVEMTPLVEIVPS
                     PLTDPACVELVCAWFEELDRQTVVLKREVPGNATGRILAAVWRECIDLVLEGVVDLAD
                     VDRLVSLGPAIEWAAAGPHLTQVMGAGARGTGVFLSEALQEYERVWGRLACWESLSGE
                     DRQRLIRLIERVYDDEPSELRAERDKFLARLLRVIEPPTEIELAFEENPDSPQSPEAA
                     P"
BASE COUNT          628 a          977 c         1004 g          492 t
ORIGIN      
        1 atcgaggtac cgggcggtgg ccaatccaag caagacgccg agtacggcac cgctaccagt
       61 gagggccaac ccttccccca gcatttgccg gatgatccgg ctccggcgaa ctccgatggc
      121 tcggagcgtg gcgatttctc ccagccgttc actgaccgta atagtcaaga gagtcgccac
      181 cagaagaaca gtgactacga gactaatcgc ccccaggatg taggagagtt gccggaagta
      241 caccagccgt tcgcggaagt gcaccaccag ctgcgcaacg ctattcactt ccacgcttgg
      301 aaactcgact cgcaggcgcg cggcaacagc gggtacggca gaatcggctc ggaccttcag
      361 cacgatcagc gaggctcgat cggttgcaag cggcccgagg agtcgctgca tcagagggag
      421 tacgacaccg acggaacgct ggtcctgata gtcgtacagc cagcgaacca agccacgaac
      481 aacgaccgat cgctctacgg ccgcagcacc aatctgcgga tccagacggc ccacgatccg
      541 gatggtgtcg cccggcccgg cctgaatggc cctggccgat gcgcggctca gaagaattcc
      601 caaagtgtct tcggcgccca ggtcacgacc ggcctccacc tgatacagga attgggcggc
      661 cggatcgatg ccgtagccga aaacagtgag aagagaatct gcgctgcggg cgtaaagcga
      721 tcccccgagg accggtgcga ccccgacgat ctccggatcg cgctcgagcc tctcgatcag
      781 cccgctcacc cgggtcacgg aggcctcggt gtcgaacgga agagtcccct tagggctcaa
      841 gcgaatctgg tagcctcggc cgagcagcat ctgttcgaac gaccgctcga tgccgccgct
      901 cagcatgacc atgtccagca gcattgcgga cgctacggcg accccaccga tggcgagcgc
      961 cgctcgcaga ggtctgcggg ccagagcccg caggctgagt ggaatcatcc ggcgcgcccc
     1021 cagagcgcaa ggggcggcgt ccgcacgaga cgccacgccg cgactgcgcc agcgccgagg
     1081 ccgagaacga gggaaagaac gacgccgaaa atcagaagcc ggggagtgac ctgcgaaaag
     1141 atcagctggg tatcgaacaa gcgacggtaa taggcgttcg ttgccgcgcc ggccgcgagc
     1201 gccatcgcga caccggctgc ggagcccagc agcgcaatga gggcggcttc gatgacgatg
     1261 gcaagaaaca ctacgcggcg acgtaccccc acgaaacgca tcactgccac gtcgttccgc
     1321 cgctcttcga ctttgagcaa catgatgcac aggagaaaaa ttgcgcttgc gaccacagtg
     1381 atcagcgcga tggcgcggtg aaaccgactc actaccagaa aagtctgcga ggatttcgac
     1441 gcgatctcgt ccgactcgaa agcgcggaat ccgaatgcga cctgattcag cttctcggca
     1501 cctcggcgtg ggtccgcgcc gggttgaatg ccaatgccga atcgatccac ccgatcctgc
     1561 gctcccagca ggcgggacag gtcgggcaga tgaagccgga catggaagtc aggtcggatg
     1621 atcccggagg gatccggtcg cggctcgtaa acggcagcga caacggctgg cgttgagacg
     1681 gagtccactg tggggcctag ccggacggta tcgccaaccc gaaggcccag ctcacgggca
     1741 agccggcgtt caacggcaat gtgaatgaga gagtcggtat tgggacttgg ggcctggatg
     1801 gccccgcaac gcggggcgcc cacgcccagg acaccaagga cagctagggc aagcaacgga
     1861 ggtttctcac acacaaagga gcgtctccca ccgagttcta ttatccttat cctacacgca
     1921 taccttccct ccctctcaaa gggacggcat ggccgattct gaccgaccgc gggttgccgt
     1981 gatcggtgcg ggccaccgcg ggcgcggctg ggctgcgctg gcgttgagcc gcggatggcc
     2041 ggtgtccctc tacgatctcg actcagcgct gcttcagcgt gccagcgccg atgtagccga
     2101 gcgagtgcgt cgactcacag atctgggtca ggccgatccg cttgccgcgg ccggtgcgct
     2161 ggactcactt cgaatcggac gcagcctgct ccacgccgtg ggtgacgccg agctcgtgct
     2221 cgatgcaatg ccgctggacc tggcaggcaa gcagcgtcta gtggaacaga tcgagcaggt
     2281 ctcgcgcgca gcttcgatca ccgtgagcat gaccggcaca atggcttcga gcgctttgtg
     2341 cgcccgactc cggcggcctg agcgccacgc cgtcgcctat gcgctcgatc ctgtcgagat
     2401 gacgccactg gtggagatcg tgccgagtcc gttgaccgat cccgcctgcg tcgagctggt
     2461 ctgcgcgtgg ttcgaggaac tggaccgcca gactgtcgtc ctcaagagag aggtgcccgg
     2521 caacgccacg gggcgaatcc tcgccgccgt ctggcgcgag tgcatcgatc tggtgctgga
     2581 aggagtggtg gatctggcgg acgtcgatcg actggtctcc cttgggccgg ccatcgagtg
     2641 ggcggcggcc gggccccatc tcactcaggt catgggcgct ggtgcgagag gtaccggagt
     2701 cttcctgagc gaagcacttc aggagtacga acgagtctgg gggcgcctgg catgctggga
     2761 gtcgctctcg ggagaagatc ggcagcggct gatccgtctc atcgaacgtg tatacgacga
     2821 tgagccaagt gagctccggg ccgaacgcga caagtttctc gcgcgcctgt tgcgcgttat
     2881 cgagccgccc accgagatcg agcttgcgtt cgaggaaaac cccgactcac cgcagtcgcc
     2941 ggaggcggcg ccgtaagaac aattggccgg gttaattcgc ctggagctta cggaaaggat
     3001 gctcgaagct gagatagagc agtagcgcgt ccgggtccgc gtcggtcagg cttacggtat
     3061 aggcaattcc gggcacgatc tgcagccctc cgcagcagtt g
//
LOCUS       DASRJQ010000316         2571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_297865, whole genome shotgun sequence.
ACCESSION   DASRJQ010000316 DASRJQ010000000
VERSION     DASRJQ010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297865"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1427
                     /locus_tag="VFB89_07075"
     CDS             <1..1427
                     /locus_tag="VFB89_07075"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM27100.1"
                     /translation="WSHDLLDEDEQRLYRRLAVFVGGWFLEAAEAVCNLDDLDVDVLD
                     GLESLAKKSLIRRVDDVDGEPRFDMLQTIREYALEQLEESGEAAHMRRCHATYWLNVA
                     EAARPELLRRNQRYWRARLEADHDNLRAAVAWTLAASGPEGAQLGARLTLALSRFWQR
                     SDHVREGREWLARALARCPPSDHGTRASLLHAAGELARAQGSNAPARSFFEASAATYQ
                     QAGDRRGTASALRNLANVIRSEGDHERASALLDESLALLRQLGERTGLAFALSIQGDL
                     ALDTGEHELAAAAYEESLSLARESGDLWSVAARLSDLGSLAHDRGDYERAYSLFNQHL
                     AMRQDVGDHRGIAWSLASLGWVACDQGDYERGRALYHQSMLLHRQLGHQWGPIVCLEG
                     LARLEATIGGSRRSPVLFGAAEAGRQMSGQALGRVEREAHEKLVAGARAALGEEVFQE
                     AWAEGSAMSLEQAVEFALETARIQ"
     gene            complement(1440..2174)
                     /gene="pyrH"
                     /locus_tag="VFB89_07080"
     CDS             complement(1440..2174)
                     /gene="pyrH"
                     /locus_tag="VFB89_07080"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HZM27101.1"
                     /translation="MARYQRVLLKLSGSAFAGNDGLGFEKAAVDLLADEILSVTSLGV
                     QVAVMVGGGNIFRGRLADQWGIERVEADNIGMMATVINSLILRGALAARGTAEVRVMT
                     AIPMSAVAEPYIRLRAVKHLERGYLVVFAAGTGQPFVTTDQPSVQRAIEIRAEVLLAA
                     KQGVDGVFTADPRLDPSARRFKTLRYDDVLARGLEVMDQAAFILARDHRLPIHVFDAA
                     SRDTMRRICEGDRIGTYIGPDVTTDF"
BASE COUNT          446 a          793 c          890 g          442 t
ORIGIN      
        1 actggagcca cgacctgctg gacgaggacg agcagcgact ctatcggcgg ctggcggtgt
       61 tcgtgggtgg ctggtttctg gaggccgccg aggcggtctg caatctggac gatctcgacg
      121 tcgacgtgct cgacggtctg gagtcgctcg ccaagaagag cctgatccgc cgcgtggacg
      181 atgtggacgg cgagccgcgt ttcgacatgc tccagacgat ccgagagtat gccctggagc
      241 agctagagga gagcggcgaa gcggctcaca tgcggcggtg tcatgcgacg tactggctca
      301 acgtggccga agctgctcgg ccggagctgc tccgccggaa tcagcggtat tggagggcac
      361 gcctggaggc ggatcacgac aatctgcggg cggcagtggc ctggacccta gcggcgtccg
      421 gaccggaggg cgcgcagctt ggagcgcgac tcacgctcgc tctgtctcgc ttctggcagc
      481 ggagtgacca cgtccgggag gggcgggagt ggctggcacg ggcgttggcc cggtgccctc
      541 cttcggacca tggaacacgc gcctcgctgc tgcacgccgc cggggagcta gctcgcgcgc
      601 agggcagcaa tgcaccggca cggtcgttct tcgaagcaag cgcggccacc tatcagcagg
      661 ctggggatcg cagggggacc gccagcgcac ttcggaacct tgcgaacgtc attcgttctg
      721 agggcgacca cgagcgcgcg agtgccctct tggacgagag cctggcgctg ttgcgacaac
      781 tcggcgagcg cacggggctc gctttcgcgc tgtcgataca gggcgacctg gcgcttgaca
      841 cgggtgagca cgagttagca gcagcagcgt acgaggagag cctgagcctc gcgagagagt
      901 ctggcgatct gtggagtgtc gcggcacgac tctccgatct agggagtctg gcccacgatc
      961 gtggggacta cgagcgcgcg tactcgctct tcaaccagca tcttgccatg cggcaagatg
     1021 ttggcgacca tcggggcatc gcctggtcgc tcgcgagtct gggctgggtg gcgtgcgacc
     1081 agggtgacta tgagcgaggc agagcgctct atcatcagag catgcttctc caccgtcagc
     1141 tcgggcatca gtggggaccg atcgtctgcc tggaaggtct cgccaggctc gaggctacca
     1201 tcggtgggtc gagacgatct ccggtgctgt tcggcgcagc tgaggccggg cgacagatga
     1261 gcggccaggc cctgggacgg gtcgagcgcg aggcccacga gaagttggtg gccggggcgc
     1321 gcgcggcgct cggcgaggaa gtgtttcagg aggcgtgggc tgaaggaagc gcgatgagcc
     1381 tggagcaggc cgtggagttt gccctggaaa cggccaggat ccagtagcca ggtcgggaat
     1441 cagaagtcgg tggtcacgtc gggaccgata taggtgccga tccggtcgcc ttcgcagatt
     1501 cggcgcatag tgtcccggct ggcggcgtcg aagacgtgga tcggcaaccg atggtcacgc
     1561 gccaggatga acgcggcctg gtccatgact tccagtccgc gtgcgagcac atcgtcgtag
     1621 cgcagcgtct tgaagcgccg ggcgctaggg tcgagcctcg gatcggccgt gaagaccccg
     1681 tcaacgccct gtttcgcggc aaggagcacc tcagcgcgga tttcgatggc gcgctggacg
     1741 gacggctgat ctgtcgtcac gaacggctgg ccggtgccgg ccgcgaagac gaccagatag
     1801 ccccgctcca ggtgtttgac cgcgcgcagg cgaatatacg gctcggcgac cgcgctcatg
     1861 ggtatggcgg tcatgacacg gacttcggct gtcccccgag ccgccagcgc cccgcgaagg
     1921 atcaaactgt tgatgaccgt agccatcatc ccgatgttgt cggcttcgac ccgctcgatg
     1981 ccccactgat cggccaatcg tccgcgaaag atattgccgc caccaaccat caccgccacc
     2041 tgaacgccca ggctggtcac cgagaggatc tcatcggcaa gcagatcaac ggccgccttc
     2101 tcaaacccga ggccatcatt gccggcgaac gcgcttccgc tcaacttcaa caggacacgc
     2161 tggtatcgag ccactcgtaa ccctcccggt caaacctcgc cagagctgtg gggctctagc
     2221 tcattctcgc tggtgccgtc gtaggttacc gacggccctg ttcttgagca gggcgcgctt
     2281 gcggctggcc atcccagggt cctactcatc cctgcagagt tagccgcacc atctggttct
     2341 cggtcgggct aacaactccc gacccccagg agtgccgttg ccacgacgtg gataccatcg
     2401 ggtcgctcat aggctcggaa tcgctccgtc agggccaccc gtgcgcgttc ggtctggacc
     2461 gcgtctagta gcccgtcagg gctgatttac agaatgttga tcgtggggct atcctgctgg
     2521 gtgcgagacg cgccgaggag ggcaggccca cgatgcggaa acagtacgtc g
//
LOCUS       DASRJQ010000317         5178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1708534, whole genome shotgun sequence.
ACCESSION   DASRJQ010000317 DASRJQ010000000
VERSION     DASRJQ010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5178
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1708534"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..1372)
                     /locus_tag="VFB89_07085"
     CDS             complement(104..1372)
                     /locus_tag="VFB89_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27102.1"
                     /translation="MALASESAGQDSAAVHNTAWLRDRLFGDTNSAFKRLGYRTLSRL
                     SQPVPPSTDTRWRRSPAVEIVVGGQQEMGLGLGLVYGPPVRPGPAPLRAEAKVLGRYG
                     VTGSYDLRLQFLAPDLVAGWRFFLLAGAERMVRTPYFGPDNKEIVEDSLKERYSILYY
                     RYALLRSTAFVAAQRHVAGPFWLHMAFQARHYRTNAIVEQPTLFAADVAAGRVDDTTR
                     YTGFEGRLGFVTDSRDDWVAPKRGAYVEALVALGRLRDDTRGTARAYHRYLVGLREFM
                     LLGGRGRTVLALRQRLVLPSNNLPYFLAYEHLTTGLPDDGVVGPRSIRLHGGGNQIAS
                     AQAFLSVDVRREAVVWRADPMNPVRLWGLLLADVGVLGEPDQSFSELRREWTIGLGGR
                     AQLARRTLVGVDVGWTDTGPGVSVVSYFAY"
     gene            complement(1399..1977)
                     /locus_tag="VFB89_07090"
     CDS             complement(1399..1977)
                     /locus_tag="VFB89_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27103.1"
                     /translation="MNRWGIAVALGQVLLPVDLGAQLGEAQFGAIGSYGTSSAWHGGA
                     GLIAGVGAGRLIYVGARWVYYFGSSAVQTDTGGTFDVRDRAQIFAADVGLQYPLGAAE
                     LVAGVTIGAMRFAQRAEPLSATGPPQSSSRVEFLLAPNLSVQLRVSSFLVIPEFVYSF
                     AGSPDQRWPVDHQGPIFSLRVVMPFEVQRIRR"
     gene            complement(1974..4940)
                     /locus_tag="VFB89_07095"
     CDS             complement(1974..4940)
                     /locus_tag="VFB89_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27104.1"
                     /translation="MALTAILLQSSLPAFAQLVRPPESGGQITSLGQPKRYRFLAGLS
                     SGLWTEEPGSSLMVRAEGGVSRHLMSPVVGLMEGGIEGFVGMRGDKPDGGLRAMLNVP
                     YLGMGVGAEYNVPDEHLNFVVGSTTPVRRGGIIRPGGMLRINWYPMESHGFTIGFMLP
                     LGDPLAGRTRPIRDYVVVARDFAPPIPYLVSNRALNEAIDSLAASAEWIRRLTVPFLD
                     QDARDTRTAEARVAAFLTGVRAHVAQRSSEQEIRYFHAQLERAFRIAAANDSIGTRMA
                     TIARRILLYQVILPYNSLLGQKKKSDELVELGIAAHGRFSREAVKSGLLSSAAMEPVL
                     YVFQRLTSIMEQERARAAKQWDDPRLVWLPLQYALLPDQYDSQEEIDALIDSITGVKF
                     TDHNEVRYLANLQFHWELLRTIKETQDYHVLWIHDFPAITSQGLLDSAALDQVVDGYL
                     TTLAERVEAYDSVGRIPMYFIFLDEHYYEARKSRIWMTILENPLHASTEIAAATKEQT
                     ARLRAALERIRAAVRDSKVLQAEARQYGDTWLRNRVKIHVNITNRADPSFWSGGLVST
                     VFGYPDNVMRDHRKIVFHDVTEADPFRGEALYTGMGVGQQYLGPTWDDRAIRVKGPAL
                     LELKRAARNLLLSQGIVESDLPTPFRLRLQPGLPGEGSVPQVADGAHVFSTRALQLSN
                     GTGYLPKPLNVGKALLYSLMGNGAVIKVPDSLWNSFLYAGLLVGACLRGSQVLIVAPA
                     ALNMPSYGAPQLTRAWELASRLLMVRDTLGGPMGAAGGMLQMGLYTLPPDQRGLASRA
                     QTWIEQVGQTVFLKGLMPFFDNAQPAVADAATRAGVAGAAPPKLHQKVQFIATGPFWS
                     TVSAAPEWRQFMETYLRYREATYNSEKSARGADTLENELSQLAGRIYQRVRGVPGAAS
                     YAIVGSQNQDYRGMFMDGEVAMVFSGAESLVPLVDVAFLEGTVTWLQDRATLDRLLPP
                     PGEYMRRLVRVGKDGL"
BASE COUNT          956 a         1721 c         1655 g          846 t
ORIGIN      
        1 gggcgccagc tcgtgcaggc ggtgcacgac agatagagga tcagcacggc acgagcggac
       61 gggcgcctga ggcaagagtt ggaggactgc acggtacccc ctttcagtat gcgaagtacg
      121 acaccacgct caccccggga cctgtgtcgg tccaaccgac gtccacgccg acgagcgtcc
      181 gcctggcgag ctgcgcccga ccgccaagcc cgatggtcca ttcgcgccgt agctcggaga
      241 aggactggtc cggctccccc agcacgccga catcggccag cagcagcccc cacaaccgca
      301 ccggattcat cggatcggcg cgccagacaa cggcctcgcg gcgtacgtcc accgacagga
      361 atgcctgggc cgaggcgatc tggttgccgc cgccgtgtag gcggatcgag cggggaccca
      421 ccacgccgtc gtccgggagt cccgtcgtga ggtgctcgta agcgaggaag tagggcaggt
      481 tgttcgaggg gagcaccagc cgctggcgca gcgcgagcac cgtgcgcccg cgcccgccca
      541 acagcatgaa ctccctcaac cccaccagat accgatggta cgcgcgggcg gtccctcgcg
      601 tatcgtcgcg caaccggccc agggcgacga gcgcttcgac gtacgcgccg cgcttggggg
      661 ctacccagtc gtcgcgagaa tcggtcacga accccaggcg gccctcaaat ccagtgtagc
      721 gggtcgtgtc gtcgacccgt cccgccgcta cgtccgccgc gaacaacgtc ggttgctcga
      781 cgatggcgtt ggtccggtag tgccgcgcct ggaacgccat gtggagccag aacggacctg
      841 cgacgtgccg ctgcgcggcg acgaacgccg tcgaacgcag cagcgcatag cgataataga
      901 ggatactgta ccgttctttg agtgagtctt cgacgatctc cttgttgtca ggaccgaagt
      961 agggcgtgcg caccatccgc tccgcgcccg cgagcaggaa gaaacgccag ccggccacca
     1021 agtcgggcgc caagaactgg agtctcaggt cgtaggatcc cgtgacgccg tagcggccca
     1081 gcaccttcgc ctcggcccga agcggagcgg ggccgggtcg cacaggagga ccgtagacga
     1141 gacccaaacc cagacccatc tcttgctgcc cacccaccac aatctcgacc gccgggctcc
     1201 tccgccaacg ggtatcggtc gacggcggca ccggctgcga aagcctggaa agggtccgat
     1261 atcccagccg cttgaaggcg ctgttcgtgt caccgaacag ccgatcgcgc aaccacgcgg
     1321 tattgtgcac cgcagcactg tcctgacccg cactctcgct cgcgagcgcc atcaacaggg
     1381 ccagtacggg aaggaacctc agcgccggat tcgttgaacc tcgaacggca tcacgacacg
     1441 gagtgagaag atcggacctt ggtggtccac cggccagcgc tggtcaggtg accctgcgaa
     1501 ggaatacacg aactcgggaa tgaccaggaa tgaggacacg cgtaactgaa cggaaaggtt
     1561 gggcgccagc agaaattcca cgcgggacga cgattggggc ggcccagttg ctgacagcgg
     1621 ttccgcccgt tgggcgaatc gcattgcgcc gatcgtcacg cccgcaacca gttccgcggc
     1681 gccgagcgga tactgcagcc cgacatcggc ggcgaagatc tgggcgcggt cccgcacgtc
     1741 gaacgtaccc cccgtatccg tttgaacggc ggagctgccg aagtagtaga cccagcgtgc
     1801 cccgacgtag atcagacggc ctgccccgac gcccgcgatc agtccggcgc caccgtgcca
     1861 ggcgctgctc gtgccgtagc tgccgatagc accgaactgc gcctcgccca gctgcgcgcc
     1921 gaggtccacg ggcaacaaca cctgaccaag ggccactgcg ataccccagc ggttcacaac
     1981 ccgtcctttc caacgcgcac caaccgccgc atgtactcgc ccggcggcgg aaggagacga
     2041 tcgagtgtcg cacgatcctg cagccaggta accgtgcctt ccagaaaggc gacgtcgacc
     2101 aacggcacca gtgattccgc tcccgagaac accatggcga cctcaccatc catgaacatt
     2161 ccccggtagt cctggttctg agagccgacg atcgcatagc tggccgctcc cgggacgcca
     2221 cgcacacgct ggtaaatccg cccggcgagc tgcgagagct cgttctcgag tgtgtcggcg
     2281 ccccgggctg acttctccga gttgtaggtc gcttcgcgat agcggaggta ggtctccatg
     2341 aactggcgcc actcaggagc cgcagaaacc gtggaccaga acgggcccgt ggcgatgaac
     2401 tggactttct ggtggagttt ggggggtgcc gcacctgcca cgcccgcgcg ggtcgccgca
     2461 tccgcgaccg ccggctgagc gttgtcgaaa aacggcatca gtcccttgag aaacacggtc
     2521 tgtccaactt gctcgatcca cgtctgcgcc cggctcgcca acccgcgctg gtccggggga
     2581 agggtgtaca gccccatttg caacatcccg cccgctgccc ccatggggcc accgagtgta
     2641 tcgcgcacca tcagcaggcg gctggccaac tcccaggcgc gggtcagctg gggagcgccg
     2701 tagctcggca tgttgagcgc tgcgggcgcc acgatgagga cctggctgcc ccggaggcag
     2761 gcgcccacca gcagcccggc gtacaggaag gagttccaca gcgaatcggg aaccttgatc
     2821 accgccccgt tccccatgag cgagtacaac agcgccttgc cgacgttcaa cggcttgggg
     2881 aggtatccag tcccattgct gagctggagt gctcgcgtgg agaagacgtg cgcaccgtcc
     2941 gcgacctgcg gcacggagcc ttcaccgggg aggccgggct ggagacgtaa gcggaaggga
     3001 gtcgggaggt cgctctctac gatgccctga ctcagcagca ggttgcgcgc ggcccgcttg
     3061 agctcgagca gcgcgggacc cttcacgcgg atcgcgcggt cgtcccacgt cggccccaag
     3121 tactgctgtc cgacgcccat tcccgtgtac agtgcctcgc ctcggaatgg atcggcctcg
     3181 gtcacgtcgt ggaatacgat cttgcgatgg tcccgcatca cgttgtcggg atatccgaac
     3241 accgtcgaca ccaatccacc gctccagaac gaaggatctg cgcggttcgt gatgttgacg
     3301 tgaatcttga cgcggtttcg aagccaggta tccccgtatt ggcgcgcctc cgcctgcagg
     3361 actttggagt cgcggaccgc tgcgcgaatt cgctccaggg ccgccctcag gcgggctgtc
     3421 tgctccttgg tggccgcggc aatttcagtc gacgcatgca gcgggttctc caggatcgtc
     3481 atccagatcc gggacttcct cgcctcgtag tagtgctcgt cgaggaagat gaagtacatc
     3541 gggatccggc cgacgctatc gtaggcctcc actcgttctg ccagcgtagt caggtatccg
     3601 tcgaccacct ggtccagtgc cgccgagtcg agcagtcctt gggatgtgat ggcggggaaa
     3661 tcatggatcc acaggacgtg atagtcctgg gtttctttta tcgtccgcag cagctcccag
     3721 tgaaactgaa gattggcaag gtagcggacc tcgttatggt cggtgaactt cacgccggtg
     3781 atcgagtcga tcagggcgtc gatctcttcc tgggagtcgt actgatcggg cagcagtgcg
     3841 tactggagcg ggagccagac caaccgcggg tcatcccact gcttcgcggc ccgggcacgc
     3901 tcctgctcca tgatcgaggt caaccgctgg aagacgtaca acaccggctc catcgcggcg
     3961 ctgctgagga ggccggactt gaccgcctct cggctgaacc ggccgtgggc ggcgatcccg
     4021 agctccacca gctcgtcgct tttcttcttc tgcccgagga ggctgttgta cggcaggatc
     4081 acctgataga gcaggatccg ccgggcgatg gtcgccatgc gggtgccgat gctgtcgttt
     4141 gccgcggcga tgcggaatgc gcgctcgagc tgggcgtgaa agtaccggat ctcctgttcg
     4201 ctcgaccgct gggcgacgtg agcgcgcact cccgtcagga acgcagccac tctcgcctcg
     4261 gcagtgcgtg tgtcccgggc gtcctggtcg aggaatggaa cggtcagacg ccggatccac
     4321 tcagccgaag cggcgagcga atcgatcgcc tcgttcagtg cccggttgga cacgagatac
     4381 ggtatgggag gagcgaagtc ccgagcgacg accacgtagt cacggatcgg tcgcgttcgg
     4441 cccgcgagcg gatccccgag cgggagcatg aacccgatgg tgaaaccatg gctctccatc
     4501 ggataccagt tgatccgcaa catcccacct ggccggatga tgccgccccg acgcacgggt
     4561 gtggtactcc caaccacgaa gttgagatgc tcgtccggga cgttgtactc tgctcccacc
     4621 cccatcccga ggtaggggac gttcagcatc gcccgcagcc cgccgtccgg tttgtcgccg
     4681 cgcatgccga caaacccctc gatgccgccc tccatcaggc caaccactgg gctcatgagg
     4741 tgacgcgaca ccccgccctc cgcccgaacc atgagcgagg agccgggctc ttcagtccag
     4801 agaccgctgc tgaggcccgc gaggaaccga tagcgcttgg gctggccgag ggaggtgatt
     4861 tggccgccgg attcgggcgg acggacaagt tgggcgaacg ctgggagaga tgactgaagg
     4921 aggattgcag tcaaagccat cgtcgggtac gctgcacgga aggtgctgcg ccacggcatg
     4981 gggtgacccc caggtactgc tgctctattc gcgggcggga cactgcgtta tctcggcgca
     5041 cctataggca atcccctacg tgtctcgaga cgaaacaaac atggcacctg ggcgcacgtt
     5101 tggttagttg gcgttcgacg cggtttccgt agcctccgtt gcgccgggtc tcacttcatg
     5161 acgacgctca gcaacccc
//
LOCUS       DASRJQ010000318         2919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1309160, whole genome shotgun sequence.
ACCESSION   DASRJQ010000318 DASRJQ010000000
VERSION     DASRJQ010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309160"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(659..>2919)
                     /locus_tag="VFB89_07100"
     CDS             complement(659..>2919)
                     /locus_tag="VFB89_07100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HZM27105.1"
                     /translation="NQVKTKLTELASSLPSGVQVVTTYDRSDLIHRSIQTLWRTLIEE
                     SIIVALVCVVFLFHARSALVAIATLPLGILVALALTRWLGINANIMSLGGIAIAIGAM
                     IDAAIVMIENLHKHIEREPERPHWERVLAASREVGPALFMSLLIITLSFLPVFTLEDQ
                     EGRLFRPLALTKTFAMGAASVLSITVVPVLMGYFVRGHVRPESANPLNRWASASYRPV
                     LRWALRARGPVVGAAVVILALTLWPLARLGSEFIPPLEEGSLMYMPNTLPSVSFAQQR
                     LLLSVQDSILMTFPEVASVWGKVGRANTATDWAPTSMTETTVNLKSKAEWRPGMTQDQ
                     LIGQMNQALRITGAVNSWTMPIKNRTDMLSTGIRTSLGVKIYGPDLRVIQRIGQEIEA
                     VLAGLHGTASIYAERSFGGRYLDIEPNAEALARYGLTTGDVQEVIATALGGETVTTTV
                     EGRERYPVQVRYAQGFRQSPDDIGRLLVSGESGVQVPLAQVAEIKFAPGPAMVRSENG
                     YLNDLVSIDVRGRDVGSYVSEGKQIIAQRVMLPPGYRLEWSGQYEALERVKARLEVVV
                     PITLAVIALLLYLNFGTLTQTLMVMLSLPFALVGGVWAMSLLNYNLSIAVAVGFIALA
                     GVAAETGVVMLLYLDHAWEDLVRDGHRPSRAELIGAIEHGAVNRLRPKLMTVAAIMLG
                     LLPALWSHGTGAEVMQRIAAPMVGGMVTSTILTLLVIPVLYFFWRGRRLEGRVGAVLW
                     GDKLPLLRKDGA"
BASE COUNT          576 a          965 c          893 g          485 t
ORIGIN      
        1 gttcacgtgg ccgtaggcgt cgaggtcgcc ccagcgcacg tcttgatcga tcaccaccgg
       61 gaagccggcg agcgcgtcgt cgcggttcat cgtcgatagc aagaatccgc gccatgaacc
      121 aggcagcggc gagattgcaa agtcaggaag gctatcaatc tcggttcgag aaccgtactc
      181 cgctagagca gccgtctggg aggcagcaga acggattgct gtggcgcaca ctcagacact
      241 gccaacggag ccttccaccg atcggccaac ggaacgagaa ttctgaacca caaggctgat
      301 cggccccagc tcaagcgcga gtggtaatac agcacacgag ggtcacaatc gtggcgaccg
      361 gagcccgtaa gccgcgtcca gaatcgaata cctcgcattt agcaggtgaa tacttcgtgg
      421 ctgctgaact ctacaagcgc ggttattcgg tcgctatgac cttggggaac gccaaggcca
      481 tcgacctatt cgcagagaaa cggcttcgaa cggttaacgt gcaggtgaaa gccatccggt
      541 tgcgccctgg ccaatcctca agaaaaaggt cttctcaaaa gtcatctacg tgttcgttcg
      601 tctaaatgat gctgaccgtc ctcccgtacc gtgtcggtct cagaggcccc catcctcgtc
      661 acgcgccatc cttgcggagc aacggcagct tatcccccca aagcacggca ccgacccgcc
      721 cttcgagcct ccgcccccgc cagaagaagt acagcaccgg gatcacgagc agagtgagaa
      781 tcgtcgatgt caccattccg cccaccatcg gagccgcgat ccgctgcatc acctccgcgc
      841 ccgtcccgtg gctccagagc gccggcaaca agcccagcat gatcgcggcg accgtcatga
      901 gtttgggccg aagccggttc accgcgccgt gctcgatcgc gccgatcagc tctgcccggc
      961 tcgggcggtg gccgtcgcgg accaaatcct cccacgcgtg atcgaggtaa aggagcatca
     1021 ccacgccagt ctccgccgcc acgccggcga gcgcaatgaa cccgaccgcc accgcgatcg
     1081 agaggttgta gttcaaaagc gacatggccc acacaccgcc gaccagcgcg aacgggagag
     1141 agagcatgac catcaatgtc tgggtgagcg tgccaaagtt gagatacaga agtaaggcga
     1201 tcaccgcgag cgtgattggc accaccacct cgagccgtgc ctttacgcgc tcgagcgcct
     1261 cgtattgccc gctccactcg agccggtagc caggaggaag catcacccgc tgggcgatga
     1321 tctgcttccc ctccgacacg tagctcccca cgtcccgccc gcggacgtcg atcgagacca
     1381 agtcgttgag gtagccgttt tcgctccgga ccatcgcggg ccccggggcg aacttgatct
     1441 cggccacctg cgcgaggggc acctgcacgc cgctctcgcc gctcacgagc aaccggccga
     1501 tgtcgtcggg gctctggcgg aacccctggg cgtaacgcac ctgaaccgga taccgctccc
     1561 gaccttcgac cgtcgtggtt accgtctcgc ctccaagcgc ggtcgcaatg acctcctgca
     1621 cgtccccggt cgtgagcccg taccgagcca gggcctcggc attcggctca atatcgagat
     1681 accggccccc aaacgagcgc tccgcgtaga tgctcgccgt gccatgcaat cctgcgagca
     1741 ctgcctcgat ctcctgcccg atgcgctgga tgacccgaag atctggcccg tagatcttta
     1801 cgccgaggct ggtgcggatg ccggtcgaaa gcatgtcagt ccggttcttg atcggcatgg
     1861 tccagctgtt caccgcgcca gtgatgcgga gcgcctgatt catctgcccg atgagttggt
     1921 cttgcgtcat tccggggcgc cactcggcct tggacttgag gttgaccgtc gtctccgtca
     1981 tcgacgtcgg ggcccagtcg gtcgccgtgt tggcgcggcc cactttgccc cagaccgagg
     2041 cgacctcagg gaaggtcatc agaatgctgt cctgcacgct gagcaagagc cgctgctgtg
     2101 cgaacgagac gctgggcagc gtgttgggca tgtacattag gctcccctcc tccagcggcg
     2161 ggatgaactc gctcccgagc cgagctagcg gccagagcgt caaggccagg atcacaactg
     2221 cggcccctac caccgggccg cgagcgcgaa gcgcccagcg gagcaccggc cggtaactcg
     2281 cgctcgccca gcggttgaga ggattcgccg actcgggccg gacgtgcccg cggacgaagt
     2341 agcccattag caccgggacg accgtgatcg agagcaccga cgccgcaccc atggcaaacg
     2401 tcttggtgag ggcgagcggg cggaacaagc gcccctcctg gtcctccagc gtaaagaccg
     2461 gcaggaatga gagcgtgatg ataaggagcg acatgaacag cgccggcccc acctcccgag
     2521 acgccgcgag cactcgctcc cagtggggtc gctcgggctc gcgctcgatg tgcttgtgga
     2581 gattctcgat catcacgatc gctgcgtcga tcatggcgcc gatggcgatc gcgatgccgc
     2641 cgagcgacat gatgttggcg ttgatgccga gccagcgggt gagagcgagg gcgacaagga
     2701 tgcccagcgg gagtgtggca atcgcaacca atgcgctgcg ggcgtgaaag aggaacacca
     2761 cgcagaccag cgccacgatg atcgactcct cgatcaacgt ccgccagagg gtttggatcg
     2821 agcggtggat caggtcggag cgatcgtagg ttgttacgac ctggaccccc gacggcagcg
     2881 acgacgcgag ctcggtgagc ttcgtcttga cctggttgg
//
LOCUS       DASRJQ010000319         5467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3910421, whole genome shotgun sequence.
ACCESSION   DASRJQ010000319 DASRJQ010000000
VERSION     DASRJQ010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5467
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3910421"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="VFB89_07105"
     CDS             complement(<1..965)
                     /locus_tag="VFB89_07105"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM27106.1"
                     /translation="MPRALPSSINLLALCSALTLLASCRSAKSDSGTQTASSGANTGT
                     ETAANPASSKTVQLTTSSPEARDLYLKGRALAEQLRAQDGRKIFEQAVAADPSFAMAH
                     YQLAVTSATAKDFFSHLKEAVALSDKASEGEQLMIKILEANGNAKPAEGLQYAEELVS
                     KFPGDERAHFLLGTAYFGRQNWDKAISEYQKSISINPSFSGAYNLMGYAYRTLEKYDD
                     AERAFRKYIELIPNDPNPYDSYAELLMKIGRFDESIAQYNKALSLDPHFGNSRIGIAT
                     NQMLQNKHDEGAAEAQKLYDAARDDGDRRFALFARSVIYVDGGRT"
     gene            1133..2752
                     /locus_tag="VFB89_07110"
     CDS             1133..2752
                     /locus_tag="VFB89_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27107.1"
                     /translation="MTSLVVLGVALRTWAYVANPPLWLDEILLARNILGLSVGDLLMQ
                     PLKLDQVAPKGFLLAEKLSVLTFGETELALRLFPFLCGIAGMFLFRSLAERILSGWAV
                     SFAVGLFAIGIPFIFHGAEVKQYELDPTAAIGLMLLTLWLLDQDRSPGQLGLAGLAGF
                     AIVWFSQTSVLVMAGLGLALAVEWGITRDRRIARVPLVTIPVWAVAGLVAIWVGIRSM
                     TPSTREFMYDFWGPGFVPRPLEPLTTGRWLWNAGLSVFTEPTLLRYRLPALFLVLAPL
                     GLVALWRQHRAVALVLAGPGLVALVAALLEQYPFRGRLMFYLVPGMLLAIAAAAEWIR
                     AAVSRIRPALGAAAMTGLALLPVAALVEAPPPYDVEHTTTLLAYLSRHRQAGDVVYVL
                     PLSRIGALFYGPSVGVMPSDWITGICDRNETRTFVRDVDRHRGKRIWLLSAGQRPYRA
                     VRQAVRSYLSTIGVKRDSLVFRSLNWESASIELYDLTDPGHLGAANAETFPVLPMPTD
                     PRPGCRPWAQPSPVDSFLIPLDRSRPTARGR"
     gene            complement(2733..3281)
                     /locus_tag="VFB89_07115"
     CDS             complement(2733..3281)
                     /locus_tag="VFB89_07115"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HZM27108.1"
                     /translation="MPNQKLTPAQRAMVDTWEAHMAAEFDTKSTDATMATMTAGPSVN
                     HVPVMTGGVGARNVSDFYSRYFIGHHPPDAKIVPLTRTVGNDRIVDELIYEFTHTIDM
                     PWMLPGIAPTGLRVRVPLVVLVDFEGGKISGERIYWDQASVLAQLGLIDRHRLPVTGA
                     EAARKVEDPAKEPSNALIARAR"
     gene            3390..3716
                     /locus_tag="VFB89_07120"
     CDS             3390..3716
                     /locus_tag="VFB89_07120"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZM27109.1"
                     /translation="MDELDPVFKALADRTRRRLLDRLFAENGQTLGELSAQLDMTRQA
                     VTKHLVVLEAANLVVTVWRGREKLHYLNPEPIEAIADRWIGKYERRRVRALRDLKQAL
                     EEKNDE"
     gene            3709..4203
                     /locus_tag="VFB89_07125"
     CDS             3709..4203
                     /locus_tag="VFB89_07125"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZM27110.1"
                     /translation="MSKPSFVYVTYIKTTAEKVWKALTDGEATRQYWFNHRNASDWKI
                     GSEWRHEDYDDPKTVDVVGTVVESDRPRRLVVTWADPTEAKNPAKISRLTYDIMEDGD
                     LVRLTVTHADLEQGSDMEKGVTSGWPVVLSSLKTMLETGKPLPAMFAREKGERKKRRF
                     ATVG"
     gene            complement(4200..>5467)
                     /locus_tag="VFB89_07130"
     CDS             complement(4200..>5467)
                     /locus_tag="VFB89_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HZM27111.1"
                     /translation="LAAVLLAVELLLFEWKPRSFIPVAAASAMAAALRVPLLGAGPIF
                     PVVLHATLPWRELALSLVIGLVAGFGSGALTWLVYTFEDLFGRLPFHTMWWPIIGGLF
                     VGLGGLLYPHALGVGYDTISGLLKGDLTGPPLLGLLIVKALIWAIALGSGTSGGVLAP
                     LLIMGGALGAVEAHWIDSSEVGLWAMISMASMMGGTMRSPLTAVAFMLELTGDIAVLP
                     ALLIACAAAHAVTVLVMRRSILTEKVARRGHHVVREYVVNPLARVRVEDAMERDVPVL
                     AVDTTLATIQQWLGDRDSLLGHRYAWPLVDQKGALVGLMTRGDLMRALARDSDKEKTV
                     LEVGASSLIVTYPDELLDEAMAKMARHNLGRLPVVHRRDPKRLLGYLGRAAIATAWAE
                     LIEEEQMREPGWLTSRARLLRRNLRRRTG"
BASE COUNT          897 a         1714 c         1829 g         1027 t
ORIGIN      
        1 ttggtacggc ctccgtccac gtagatcacc gaccgagcga agagcgcgaa cctgcggtcg
       61 ccgtcgtcgc gcgccgcgtc gtacagcttc tgcgcctcag cggctccctc gtcgtgcttg
      121 ttctggagca tctggttggt cgcgatgccg atacgggagt tgccgaagtg cggatcgagc
      181 gagagcgcct tgttgtactg cgcaatggac tcgtcgaacc ggccgatctt catgagcagc
      241 tcggcatagg aatcgtacgg gttggggtcg ttggggatca gctcgatgta cttccggaag
      301 gcccgctccg cgtcgtcgta cttctcgagc gtccgatagg cgtagcccat caggttgtag
      361 gcgccggaga agctggggtt gatggagatc gacttctggt actccgagat agccttgtcc
      421 cagttctgcc ggccgaagta ggcggtgccg aggaggaaat gagcccgctc gtcaccgggg
      481 aacttgctca ccagctcctc ggcgtattga agaccctctg ccggcttggc attgccgttg
      541 gcttccagga tcttgatcat caactgctcg ccctcggacg ccttgtctga gagtgcgaca
      601 gcttccttga gatgagaaaa gaagtccttc gccgtcgccg aggtgacggc gagctggtag
      661 tgcgccatgg caaacgacgg atctgccgcg acggcctgct cgaagatttt gcgtccgtcc
      721 tgggctcgga gctgctctgc aagcgcacga cccttgagat agagatcgcg ggcttcgggg
      781 gagctggtag tgagttgaac ggtcttggag gaggcgggat tggcggcggt ttcggttccg
      841 gtattggctc ctgagcttgc ggtctgagtc ccggagtcgg acttggcgga gcgacagctt
      901 gcgagcaggg taagcgcgct acagagagcg agaagattga ttgacgaggg caacgcgcgg
      961 ggcatgggaa tcctccggca acgtttcagt tgtgtacgac ggagtagttc ggtgggaaca
     1021 gggaacctta cgcggccggg ggaggggacg gtttcagccg cggctcgcgg tttcaaccgg
     1081 ggagaatagg ttcgttgccg tgacgatgcc ggcgccatct actcggacgg tcgtgacctc
     1141 tctggtcgtt ctcggagtag cgttgcgcac ctgggcctac gtcgccaacc cgccgctctg
     1201 gctggacgag atcctgcttg cccgcaacat ccttgggcta tcggttggcg acctcctcat
     1261 gcagccgctc aagcttgatc aggtcgcgcc gaagggcttt ctgctggccg agaagctttc
     1321 cgtcctcacc ttcggcgaga ccgagttggc cctccgcctc ttccccttcc tgtgcggcat
     1381 cgcgggcatg tttctcttcc gaagcctggc cgagcggatc ctctcaggat gggccgtgtc
     1441 atttgccgtc gggcttttcg cgatcggaat tcccttcatc ttccacggcg ccgaagtcaa
     1501 gcagtatgag ctggatccca ctgccgcaat cggtctaatg ctgctcacac tttggttgct
     1561 ggatcaggac cgctcgccgg gccagctggg cctcgcaggc ctggccgggt ttgcgatcgt
     1621 ctggttctct caaacctcgg tcctcgtgat ggcgggcctc gggcttgcgc ttgcggtgga
     1681 gtggggcatc actcgggatc gccgcattgc tcgcgtaccg ctggtgacta ttccggtgtg
     1741 ggccgtggcc ggcctcgttg cgatttgggt cggcatccga agcatgactc cctcgactcg
     1801 cgagttcatg tacgacttct gggggcccgg gttcgtcccg cgcccgctgg agccgctcac
     1861 caccgggcgg tggctctgga acgccgggct ctccgtcttc actgagccca cactgctccg
     1921 atatcgcctg cccgcattgt ttctggtgct ggctccgctt ggactggtcg cgctgtggag
     1981 gcaacaccgg gccgttgctc tcgtgctggc cgggccggga ctggttgccc tggttgctgc
     2041 cctgctcgag cagtatccgt ttcgcggtcg gctcatgttc tatctggtgc cgggcatgct
     2101 gctcgccatc gctgcggctg cggagtggat tagggcagcc gtttcgcgaa tccggccggc
     2161 gctgggcgcc gcggccatga ccgggctcgc tctactaccg gtggcggcgc tggtagaggc
     2221 tcctccgccg tacgacgtcg aacacacgac gacgttgctg gcatatctct cccggcaccg
     2281 gcaagccggc gatgttgtgt acgtgcttcc gctctcgaga atcggtgcgc tgttttacgg
     2341 gccgagcgtt ggggtgatgc cgagcgactg gatcacggga atctgcgacc ggaacgagac
     2401 ccggacattc gttcgcgacg tggaccggca tcgtggaaaa cgaatttggt tgctttcagc
     2461 ggggcagcgc ccctatcgcg cggtgcgcca ggccgttcgg agctatctca gtactatcgg
     2521 cgtgaagcgg gattcgctgg tctttcgatc gcttaactgg gaatccgcga gcatcgagtt
     2581 gtacgatctc accgatcctg ggcatctcgg cgcagccaat gcggagacgt tcccggtctt
     2641 acccatgccg accgatccca ggccgggctg ccggccctgg gcgcagccgt ctcctgtcga
     2701 cagcttcctg atcccactcg atcgaagtcg gcctaccgcg cgcgggcgat gagcgcgttg
     2761 gagggctcct tggccggatc ctcgactttt cgcgcggctt cggcgccggt caccggcagt
     2821 cggtgccggt cgatgaggcc gagctgtgcc agcaccgagg cctggtccca gtagatccgc
     2881 tcgccgctga tcttcccacc ctcgaaatcg accagcacca ccagcgggac tctcacgcgg
     2941 agtcccgtgg gagcgatgcc gggcagcatc cacggcatgt cgatcgtgtg ggtgaactcg
     3001 tagatcagct catccacgat ccgatcgttg cctaccgtgc gggtgagcgg gacgatcttc
     3061 gcgtccggcg ggtggtggcc aatgaagtat cggctgtaga agtcactcac gttcctggct
     3121 cccaccccgc ccgtcatcac cggcacatgg ttcacgcttg gccctgcggt catggtcgcc
     3181 atcgtggcat cggtgctttt cgtatcgaac tcggcggcca tgtgtgcctc ccaggtgtcc
     3241 accatggcac gctgcgccgg ggtcagcttc tggttcggca tgggtgtgtc cctcgatagc
     3301 tggggtgcgc ggaccggccg aatctgtcgt cctggccggc caggcgccat cccttgacat
     3361 gcaaccttag ggttgcatat tagtcgccca tggacgagct ggacccggtg ttcaaggctc
     3421 tcgccgaccg gacccgtcgc cgtctgctcg accggctgtt cgcggagaac ggccagaccc
     3481 ttggcgagct ctccgcgcag ctcgacatga cccgacaggc cgtgaccaag cacctcgtgg
     3541 tgctcgaagc ggcgaatctg gtggtgacgg tgtggcgtgg gcgggagaag ctgcactacc
     3601 tcaaccccga gccgatcgaa gcgatcgccg atcgctggat cggcaagtac gagcgccgga
     3661 gggtgcgagc gctacgcgac ctcaagcagg cgctggagga gaagaacgat gagtaagccg
     3721 agtttcgttt acgtcaccta catcaagacc accgccgaga aggtgtggaa ggccctcacg
     3781 gacggggagg cgacgcggca gtactggttc aaccaccgca acgcatccga ctggaagatc
     3841 ggctccgagt ggcggcacga ggactacgac gacccgaaga ccgtggacgt ggtgggcacc
     3901 gtggtcgaga gcgaccgtcc tcgccgcctg gtggtgacgt gggccgatcc cacggaagca
     3961 aagaacccgg cgaagatatc gcggctcacc tacgacatca tggaagacgg cgacctggtg
     4021 cggctcaccg tgacccacgc cgatctcgag cagggctcgg atatggagaa gggcgtaacc
     4081 tcgggctggc cggtggtgct ctcgagtctc aagaccatgc tcgagaccgg gaagccgttg
     4141 ccggcgatgt ttgcccgcga gaagggggag aggaagaagc ggcgcttcgc gacggtgggc
     4201 tagccggttc gcctgcggag attccggcgc aggagacgag cccggctggt cagccagccg
     4261 ggttcgcgca tctgctcctc ttcgatcagt tcggcccacg cggtcgcgat tgcggctcga
     4321 cccagatagc ccaacagccg cttcggatca cgacggtgga ccaccgggag ccggcccagg
     4381 ttgtgtcgcg ccatcttggc catggcctcg tccaacagct cgtcgggata ggtgacgatc
     4441 aaggacgagg ccccgacctc gagcacggtt ttttccttgt cgctgtcgcg cgcgagcgcc
     4501 ctcatgagat cgccgcgggt catgagaccg accagcgcgc ccttctgatc tacgaggggc
     4561 cacgcatagc gatgacccag cagcgaatcc cggtcgccga gccactgctg aatggtggcg
     4621 agtgtcgtgt cgacggcgag cacgggcaca tcgcgctcca tcgcgtcctc cacccgcacc
     4681 ctcgcgagcg gattgaccac gtactcgcgc acgacgtgat gtccccgccg ggccactttc
     4741 tcggtgagaa tcgagcggcg catcaccagc acggtgacgg cgtgcgccgc cgcgcatgcg
     4801 atcagcagcg ccggcaatac agcgatgtcg ccggtcagct ccagcatgaa cgcgaccgcc
     4861 gtgagcggcg accgcattgt gccacccatc atcgacgcca tactgatcat ggcccagagc
     4921 ccgacctcgc tggagtcgat ccagtgcgcc tcgaccgcgc cgagcgcgcc gcccatgatg
     4981 aggagcggcg caagcacgcc tcccgatgtg cccgagccga gtgcgatggc ccagatcagc
     5041 gccttgacga tcagcagtcc aagcagcggt ggtccggtga ggtccccttt gagcagtccg
     5101 gaaatggtgt cgtagccgac gccgagggcg tgggggtaca gtagtccgcc gagcccgacg
     5161 aacagcccac caatgatggg ccaccacatc gtgtgaaacg ggagtcgccc gaagaggtct
     5221 tcgaaggtgt agaccagcca tgtgagggcg ccggagccga agccggccac gagcccgatc
     5281 acgagcgaga gggccagctc gcgccagggc agggtggcgt ggagcacgac tgggaagatc
     5341 gggccggccc cgagcagcgg gacccggagt gctgcagcca tggcggacgc cgcggcgacg
     5401 ggaatgaagc tccggggctt ccactcgaaa agcagcagct ccaccgcgag cagcactgcg
     5461 gcgagcg
//
LOCUS       DASRJQ010000320         2443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1424901, whole genome shotgun sequence.
ACCESSION   DASRJQ010000320 DASRJQ010000000
VERSION     DASRJQ010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424901"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1527
                     /locus_tag="VFB89_07135"
     CDS             82..1527
                     /locus_tag="VFB89_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27112.1"
                     /translation="MSAKRRGSWLSPSSPTRTSGGDRLSGASTIAPGRVETHGELDHL
                     LFRPRLHPDVVVRRLESYRDGPYYILKSPQSGAYLRLGPREHEFLDLMDGQRTITEIA
                     VEGFYRHRRLGLALVAQLTELLHREGFLITAPEVAPLVSQAPSPPDKPAARVRRLVAR
                     LFRDEVTLPGIDAFVTRLYASVGRLLLARPAIALYSLVALCGLLTFLTSHRLQPVDLF
                     APRGIEMLFLVALFVVLLIFCHELAHALVCKSFGREVRRGGFALYFGIPIFFVDTTDI
                     WLENRGRRIAVSAAGPFSDLTLGGACSLLAVALPVDAADAVLFLASIAYLAFLANLIP
                     LLKLDGYYILVDLLEIPMLGPRSFAFVREHLLGKLWRRERFDREELLFACYGLLAGLF
                     AVLMFFYAVRLLEMQLAGLIDSLARGGSWLVAPLALLLLIRLGIPLLLRATRALTDTA
                     RLATHTLRMLDNDQRPNVDRSPSIVDPLGKQ"
     gene            complement(1616..2080)
                     /locus_tag="VFB89_07140"
     CDS             complement(1616..2080)
                     /locus_tag="VFB89_07140"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZM27113.1"
                     /translation="MKPRLNSARAAPGAMRALAALDTYVRQSGIEPNLIELVKTRASQ
                     INGCAYCLHMHTRDARAKGESEERLYVLAAWRESPLYTERERAALAWTEAVTLVSQSG
                     VPDDVYDEASRHFSKTELVNLTVAVAAINAWNRIAISFRAVHPVNPTHAARA"
BASE COUNT          382 a          821 c          731 g          509 t
ORIGIN      
        1 tggatgcctt cgacctcacc gaggccgaga aggaagcatt gcgcacccag gactccgacg
       61 ccctcgactc catgagcgta gatgagcgca aaacgccgtg gttcctggct aagtccatct
      121 agcccgacgc gcacgtccgg cggcgatcgg ctcagcggcg cctccaccat tgctcccggc
      181 cgggtggaga cccacggtga actcgatcac ctgctgttcc ggccgcggct gcacccggac
      241 gtcgtggtgc gccgtctcga gtcgtatcgt gatggtccct attacatttt aaagagcccg
      301 cagagtggcg cctatctacg cctgggccca cgcgagcacg agtttctcga cctgatggat
      361 ggccagcgga ccatcaccga gatcgccgtc gagggctttt atcggcatcg ccgcctggga
      421 ctcgcgctag tcgcccaact gacggagctg ctccatcggg agggcttcct gatcaccgcg
      481 cccgaggtgg cgcctcttgt gagccaggct ccatccccac cggacaaacc cgcggcgcgg
      541 gtcaggcgac tcgttgctcg gctgttccgg gacgaggtta ctctgcccgg catcgatgcg
      601 ttcgtcacac ggctctatgc gtcggtcggc cgactgttgc tggcccgacc cgccatcgcc
      661 ctctatagcc tcgtcgctct ttgcggcctc ctgacatttc tgacctcgca ccgccttcaa
      721 ccggtggacc tgttcgcccc ccgcggcatc gaaatgttgt tcctggtcgc cctgttcgtt
      781 gtgctgctga tcttctgcca cgagctggcg catgccctgg tctgcaagtc gttcggccgc
      841 gaggtgcgcc ggggcggctt cgccctgtat ttcggcatcc ccattttctt cgtcgatacg
      901 actgacatct ggctggagaa ccgcgggcgt cgcatcgccg tctcggcggc cggacccttc
      961 tcggacctca cgctcggcgg agcctgctcg ctcctggcag tggctctgcc ggtggacgcc
     1021 gccgacgcgg tcctgttcct cgccagcatt gcctacctcg cgttcctcgc gaacctaatc
     1081 cccctcctca agctggatgg ctactacatc ctggtagacc ttcttgagat acccatgctc
     1141 gggcctcgct cgtttgcctt tgtccgggag cacttgcttg gcaagctctg gcgaagagag
     1201 cgcttcgatc gggaggagct gctattcgcc tgctacggcc tcctggccgg gcttttcgcc
     1261 gtcctgatgt tcttttatgc cgtccgactc ttggagatgc agctcgctgg cctgatcgac
     1321 agcctcgcca ggggcggcag ctggctggtg gcgcccctcg ccctgctcct tctcatccgg
     1381 cttggcatcc ccctcctcct ccgagcgacc cgggcgctca ccgacaccgc ccgcctcgcc
     1441 acccataccc tccgaatgct ggacaatgac cagcggccga acgtcgatcg atcaccatcc
     1501 attgtcgacc cattagggaa gcaatgaagg atttcttcag gggggccttc cggccaaagc
     1561 caccgatttc actgaagcac gcagcggacg aaggattgta tcgtgcgcaa gcctgttacg
     1621 ctcgggctgc atgtgttgga ttgaccggat gcacagcgcg gaagctgatt gcaatacggt
     1681 tccacgcgtt gatcgcggca accgcgacgg tcaggttcac cagttcggtt ttggagaaat
     1741 gtcggctggc ctcgtcgtag acgtcgtcgg gaaccccact ctgcgacaca agtgtcaccg
     1801 cctcggtcca ggccagcgcg gcgcgttcgc gctcggtata gagtggtgac tcgcgccagg
     1861 cagcaagtac gtatagccgc tcttcgctct cccctttagc gcgcgcatcg cgggtatgca
     1921 tgtgcaggca gtaggcgcag ccgttgatct gcgaggcgcg agtcttgacc agctctatga
     1981 ggtttggctc gatgccgctc tgtcggacat aggtatcgag cgcggccagt gccctcatgg
     2041 caccgggcgc cgcccgggcg gaattgaggc gcggcttcat cgtcgtcctc ctatgctcga
     2101 gggggcggcg caatggccgc cgatctccca atggtccgct ttcttaagca gcagtgcggg
     2161 aaacagagcg atgatccagc ccgcgctcag cgtcacgatg gcgcatgcct tgggccagac
     2221 ggcgctcgat ggtttccgtc tccctgacca acatcgatta cccgatcctc gacccgccgg
     2281 ggaagcacga aggcgcggcc cggaatccac ggcgtttcta ggcctggaag ttcagacgct
     2341 cgcgggtagc atcggtggtg agcagaatgc gcagcggatc ttcggtgttg aagctacgct
     2401 caatctcttt acgtcgtgca ctgctggtta gaccgcgaat cac
//
LOCUS       DASRJQ010000321         2434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2309457, whole genome shotgun sequence.
ACCESSION   DASRJQ010000321 DASRJQ010000000
VERSION     DASRJQ010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2309457"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VFB89_07145"
     CDS             complement(<1..708)
                     /locus_tag="VFB89_07145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZM27114.1"
                     /translation="MTEVRGEQKQGLSEGLLLGGLIVVAFVLRFARLGLWSLDSDEVF
                     MRRDSLNFRFTNPRPLLYFINHHVIAPIIPLDEFGIRLLPATFGVLAIPVLYLVTRRF
                     FGARAALFAAALLAVSPVHVSYSQFGRYWSLVFLLATIYPYALYLGARGRDGRALALG
                     IATALLATLAHPSAVLLMGGPAALLAFHYLRPDRLKQLWALPAFRWAALAAVVVAVAI
                     LIRFIPILHGWIYEHDAN"
     gene            1231..2097
                     /locus_tag="VFB89_07150"
     CDS             1231..2097
                     /locus_tag="VFB89_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27115.1"
                     /translation="MFTVAACGDSVDPTSPDTGDDVMTVVPAGNEPLASAFAGGIPFG
                     NFAQPISQLGSIFNGSHENSTPTGLLRELQETKSRGGKVILALSGSPRYYTDGAGNFS
                     FTEWKERVNRFRNVNFRSYIEDGTIVGHYLIDEPNDPANWNGKEVSSSMVEEMAKYSK
                     SIWPTMATIVRAEPRHMGDNHRYLDAAWAQYLYRRGNVNEYIRDVVSTAQRKGLALIV
                     GMNVLKGNRNGGRMSPSEVEDWGSALLSSSYPCAFINWKYSNDMTSGSMRNAMSALRR
                     KAENRSNKSCRA"
BASE COUNT          465 a          804 c          771 g          394 t
ORIGIN      
        1 gttcgcatcg tgctcgtaga tccatccatg gagtatgggg atgaaccgaa tcaggatcgc
       61 gacggccacc acgaccgcgg cgagcgcggc ccaccggaac gcgggcagcg cccacagctg
      121 cttcagccgg tcgggccgca gatagtgaaa ggcgagcagg gccgccggcc ctcccatgag
      181 gaggaccgcg gagggatgcg ccagcgtggc cagcagggcg gtggcgatgc cgagcgccag
      241 cgctcgtccg tcgcgccccc gtgcgccgag atacagtgcg taggggtaga tcgtggcgag
      301 caggaacacg agcgaccagt accggccgaa ctgcgaatag ctgacgtgca ccgggctcac
      361 ggcgagcagc gccgcggcga agagtgcggc ccgcgcgccg aagaatcggc gcgtgaccag
      421 gtagagcacc gggatcgcga gcaccccgaa ggtcgcgggc agcaacctga tcccgaactc
      481 atccagggga atgatcggcg cgatcacgtg gtggttgatg aagtaaagca ggggcctcgg
      541 attggtgaat ctgaaattga gcgagtcccg ccgcatgaag acttcgtcgc tgtcgaggct
      601 ccacaggccg agccgcgcga agcggagcac gaacgccacg acgatcaaac cgccgaggag
      661 caggccctcg gagagtccct gcttctgttc accgcgcact tcggtcatcg gtatggctcg
      721 tggaggaagc gctcgaagtg gagcggctac accgctcgcg cagcaaatgt tacgaggcga
      781 ggtctggttt ggaagcccgg gccgtccgat gtcaaatcct ttgacgatat caccgtgagc
      841 atacgctcgc ggtgacgttg ggtcttgaca tccgtgacga agatcaccat tctcttcccc
      901 ggcgcgagct ctgaccacga gatgacacca cccgggtgct cattccttcg cgccctgctc
      961 gaccagattt tccggtcgca gattgacccg caaggtctcc acacagtcat agagacaggc
     1021 agacaacctg ccgcgtctca ccagtcctag gctcaaccta cgaagagccg ccgcccctgg
     1081 ccgttgccgg gcgctggccc ctcgtctgag caacccctgt gtccacgccg tcctcgtggt
     1141 cggcggagag aggaacgcct gcgtatgccc ttcagtctcg agtcgtgtcg ccgagtcacg
     1201 cgccttcgaa ctctcgttct tgtcacttcg atgttcaccg tcgctgcgtg cggcgattcg
     1261 gtcgacccca ccagccccga caccggcgac gacgtcatga ccgtggtccc ggccgggaat
     1321 gagccccttg cctccgcgtt cgccggcggc attccgttcg gcaatttcgc tcagcccatc
     1381 agccaactcg gctcaatttt caacggttcg cacgagaact ccacccccac cggactgctc
     1441 cgcgagctgc aggagaccaa gtcgcggggc ggtaaggtca tcctggcgct ttcgggaagc
     1501 ccgaggtact acaccgacgg ggccgggaac ttcagcttca ccgagtggaa ggagcgggtg
     1561 aaccgcttcc ggaacgtcaa cttccgctcc tacatcgagg acggcacgat cgtcggccac
     1621 tatctcatcg atgagccgaa cgatccggcc aactggaacg gcaaggaggt ttcctcctcg
     1681 atggtcgagg agatggccaa gtacagcaag tcgatctggc ccaccatggc caccatcgtg
     1741 cgtgccgagc cgaggcacat gggggataac caccgctacc tggacgccgc gtgggcgcag
     1801 tatttgtatc ggcgcggcaa cgtcaacgag tacatcaggg acgtcgtcag caccgcgcaa
     1861 cgcaagggtc tggccctcat cgtcggcatg aacgtgctca agggcaaccg caacggcggc
     1921 cggatgagcc ccagcgaagt ggaggattgg ggctccgcgc tgctgagcag cagctatccc
     1981 tgcgccttca tcaactggaa gtactccaat gacatgacct ccggctcgat gcggaatgcg
     2041 atgagcgctc tgcgccgcaa ggcggagaat cgaagcaaca agagctgcag ggcctgagcc
     2101 gggaggcggg caggcgccga gtccgagggc cgcggagatt cgcggccccc gggtttttca
     2161 ggggttgaca ccggtgcgcc gggtccccat tctccacccg gcgcgagcta caaagacgac
     2221 gagatgacac caggtcggtg ctcattcctc cgcgccccgc agggctagtt ctctagtttt
     2281 cagcggtccc gtacctctca catacccaga caactcgagt gatcgggaat ccggcagcgc
     2341 cgctgccgcg tcccgccgtg caggtcttcg cgcccaccgc ggcctcgccc cgcgatgccc
     2401 cgagtgatgg cctccctgcg cgcgaccttc gtac
//
LOCUS       DASRJQ010000322         3258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1968918, whole genome shotgun sequence.
ACCESSION   DASRJQ010000322 DASRJQ010000000
VERSION     DASRJQ010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1968918"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..958)
                     /locus_tag="VFB89_07155"
     CDS             complement(20..958)
                     /locus_tag="VFB89_07155"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HZM27116.1"
                     /translation="MGLDPKLHDRIPRARRAVASVILAAASFVAPLGLLQGCHAAPVG
                     QAGTTIADDAGDSVKLTLPVHRIVSLNPAATELLFAIGAGNVVVGRTTWCDYPPEAQA
                     VPSLGDGINPNLEAIVARKPDLVLLYSSSQNADAVQRLAQLGIPALRLRSDSLSDVPR
                     LARLFGRLTGRTEAADSVSRTFETELAKATVHPEGARPTVFLLVWDQPPMTVGRGSFL
                     TELLERAGAENLFADVATSSAPVSIEAVAARNPNVILALGVETPAFLSRPEWQVVPAV
                     RQRRLVREEGSEFSRPSPRAPAAIREFREAVAEAME"
     gene            complement(942..1940)
                     /locus_tag="VFB89_07160"
     CDS             complement(942..1940)
                     /locus_tag="VFB89_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27117.1"
                     /translation="MPSVRVLLHRVGRNAQGPLDSTVTDAAGRFRFRFVPDTGAVYLT
                     STRYQDIEYFSPAVHTNPALPDTALFIIVSDTSSSAPVDLEARHLVVADPGEDGNRGV
                     LDLIILRNRGDHTRVSSDTSRPSWSAPLPNGTFGLEPGESDVSPEALRRVGNRVELLA
                     PLAPGEKQLVLQYGLPAAVHGLELPFEEAADLVNVLIADRGARVSGGNLAPADTEVIQ
                     GRTYQRWMGPVAAGAVIRIRLSRPGYAPRWVLPSLVALVGTILLVAGIRIFRIRSQPG
                     WAASSDDADVLVERLAQLDARYAGREVDVAPSEWERYRAERATLKARLESLLGTRS"
     gene            2385..2546
                     /locus_tag="VFB89_07165"
     CDS             2385..2546
                     /locus_tag="VFB89_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27118.1"
                     /translation="MSRQETATCDQMGLYTPVSLYDAAAGNVVGIEIDPLAAGQVFSL
                     KALNVTIFP"
     gene            2767..3138
                     /locus_tag="VFB89_07170"
     CDS             2767..3138
                     /locus_tag="VFB89_07170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZM27119.1"
                     /translation="MARILVIDDEAVVRRAIVRMLAAGGHDAVEAPDAATGLRLWDEQ
                     GADVIVTDISLPDMTGFELIGLIRSEGETVPIVAISGSLVVSDLELIAERLRIVEILA
                     KPFSGDRLLSAVSKALADKAR"
BASE COUNT          613 a         1006 c         1057 g          582 t
ORIGIN      
        1 agcagcagac cgagttgctt cattccatgg cctctgcgac ggcctctcgg aactcgcgga
       61 tcgccgcggg cgctctcggg ctcgggcgac tgaactccga tccctcctct ctcaccaggc
      121 gtcgctgacg tactgcgggc acgacctgcc attccggtcg actcaaaaac gccggagtct
      181 cgacgccgag cgccagaatg acgttgggat ttctcgccgc aacggcttcg atgctcaccg
      241 gcgcggacga cgtggcgaca tccgcaaaca ggttctccgc tcccgcgcgt tcgagcagtt
      301 ctgtgagaaa gctgccccga cccacagtca tcggcggctg atcccacacc agaagaaaga
      361 cagtgggccg ggcaccttca gggtggaccg tcgccttggc cagctccgtc tcgaaagttc
      421 ggctcactga atccgcggct tccgtgcggc cggtgagccg gccgaacagg cgagctagtc
      481 ggggcacatc actgagcgag tcggagcgga gccgcaatgc gggaatgccg agctgcgcca
      541 gccgctgcac ggcgtcggca ttctgggacg aggagtacag caggacgaga tcgggtttcc
      601 gcgcgacaat cgcctcgagg ttcgggttga taccgtcgcc cagacttggg accgcctgcg
      661 cctcgggtgg atagtcgcac caggttgtgc gacccacgac gacgttccca gctccgatcg
      721 caaagagcag ctcagtggcc gccgggttga gcgatacgat acggtggacg ggtaaagtca
      781 gcttgacgct atcgccagcg tcgtcggcga tcgtggtgcc ggcctggccg acgggcgcgg
      841 catggcagcc ctgaagcaaa ccaagcggag ccacgaaaga ggcagccgcc aagatgaccg
      901 aagccaccgc acggcgagca cgcgggatgc ggtcgtgcag cttaggatcg agtcccaagc
      961 aagctctcca gtctggcctt gagtgtggcc cgctccgccc ggtaccgctc ccactctgat
     1021 ggtgccacgt caacctcacg accggcatag cgtgcgtcca actgagccag acgctcgacc
     1081 agaacatctg cgtcgtcgga gctggctgcc cagccaggtt gggaccggat tcggaaaatc
     1141 cggatgccgg ccacgagcag gatcgttccc acaagcgcaa ccagcgatgg cagtacccat
     1201 cgaggcgcgt agcccggccg actgagtcgg atccggatca cggctccggc cgccaccggg
     1261 cccatccagc gctgatatgt gcgcccctga atcacctccg tgtcggccgg ggccaggttg
     1321 ccgccgctga cccgggcccc acgatcggcg atcaggacgt tgaccagatc cgcggcctcc
     1381 tcgaatggaa gctcgaggcc gtgtacagct gcgggcagtc cgtactggag gactaattgc
     1441 ttctctcccg gcgcgagcgg ggcgagcagc tcgacccgat ttccgacccg acgcagcgcc
     1501 tccggcgaca cgtcactctc acccggttcc aaaccgaagg tgccatttgg cagcggggcc
     1561 gaccacgatg gacggctggt atcagacgag acgcgcgtgt gatcgccgcg gtttcggaga
     1621 atgatgaggt caagcacgcc gcggttgccg tcctctcccg ggtctgcgac cacgaggtga
     1681 cgcgcttcca agtcgacagg ggcggatgac gaggtgtcgg atacgatgat gaagagcgca
     1741 gtgtccggga gcgccgggtt ggtgtgcacg gcgggtgaga agtattcgat gtcctggtaa
     1801 cgcgtgcttg tgagatacac ggctccggtg tcaggcacaa agcgaaaacg gaaacgcccg
     1861 gcagcgtcgg tgacggtgga gtcgagcggg ccctgagcgt ttctccccac tcgatggaga
     1921 aggacgcgaa ccgacggcac cggaaccgaa tcctttccca ctgttcggac cacgcggcca
     1981 tcggctcgga tcggagtttg agcgcggaga gggacgacgg cggatagcac tccaagggca
     2041 actgcgagat ggcggatgac ggcggtcgga ggcaaccggg atggcgtccg atgcgattgc
     2101 tgggacgctc gcgccaactt gcttccgttt ccgacggctg tctgtcgatg gattgaactt
     2161 tcgttactcc agtggcttac tgtctggtag gacaatggtt tgcgggtcgg cacggaggag
     2221 aaatgtggcc aaactagggc caaacgccaa gctggttgcg cggaagcagg ctagctcgag
     2281 gaggatcgtc taaccaagcg ggcagctgta ggccccgccg cagcagtagc gagggcgctt
     2341 tgaccttctg ctggccgaac tgctcaggcg gagctaggct gaggatgtcg cggcaggaaa
     2401 cggcaacctg tgaccagatg ggcctttaca caccagtctc gctttatgat gccgcggctg
     2461 gtaacgtcgt cggcatcgag attgatcctc ttgccgcagg ccaggtcttt tcacttaagg
     2521 cgctgaacgt caccatcttc ccctgaagct gagccatagc ctttggccct ttctagacgc
     2581 acaccagctc tgtgcggccg gggctggtga tgttgggctc cggccgtagc tttgccgtac
     2641 cgaatgcacc cacgacgggg accggctcac gcgccggacg gcgggcgcaa ggcgctggcg
     2701 gagtacggtt ctcgaactga gattagatag cctccccatt ttgcaattct caccgctgcc
     2761 gggctcatgg cgcggattct cgttatagac gatgaggcag tcgtccgccg tgcgatcgtc
     2821 cggatgctcg cggcaggtgg gcacgatgcc gtcgaggctc ctgatgctgc gacgggacta
     2881 cgcttgtggg acgaacaagg tgcagacgtc atcgtgacgg atatttcgct gcctgatatg
     2941 acaggtttcg agctaatcgg cttaatccgc tcggaaggcg agacagttcc cattgtcgcg
     3001 atctccggca gcttagtggt tagtgatctc gaattgatcg ccgagaggtt gaggattgtc
     3061 gagatcttag caaagccgtt ttccggggat cgactccttt cggccgtgag caaggcactt
     3121 gccgacaagg cccgctaaga aggttcgaac gccgactgaa ttaccgaccg ttgcacccat
     3181 aattccagtg ctgcgatccc atccccctgc ttttccgtac cgaatgcgcc cacgacccgg
     3241 accggcctac gcgccggc
//
LOCUS       DASRJQ010000323         2746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_369717, whole genome shotgun sequence.
ACCESSION   DASRJQ010000323 DASRJQ010000000
VERSION     DASRJQ010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369717"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1217)
                     /locus_tag="VFB89_07175"
     CDS             complement(<1..1217)
                     /locus_tag="VFB89_07175"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HZM27120.1"
                     /translation="MPFETTVAAKPPATVETPLLGIAVPQGTGLPGSLGEIDRATGGA
                     LARLYAAGDFTGKRDETTVLYPTGPAARIVLVGLGKIEGTAAPDVRRAAAIIAKRARS
                     LGAPSGTFYLAPEGRGPLSWRDVGQFAAEGLAHGAWIFTEMKKPPEDPKPVLERFTIL
                     APEATAEVEAGHQIGAAIGAGQTFTRGLQVLPGNVCTPTFLGKTAEELAQRHGFAVTV
                     LDKAAIVREKMGALLSIAQGSAEEPRFIALEYKGSDAKPVVLVGKGVTFDTGGISIKP
                     AQNMEDMKFDMSGAAAVLGTFEMLGRLKPKVHVVGLIPSTENMPSGTAVKPGDVITSH
                     FGKTIEMINTNAEGRLILCDALSYARRYQPAAVIDIATLTGAIVIALGHTASGLMGND
                     EALIEEVRAAGDRA"
     gene            complement(1244..2380)
                     /locus_tag="VFB89_07180"
     CDS             complement(1244..2380)
                     /locus_tag="VFB89_07180"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HZM27121.1"
                     /translation="MTSPVGSAAFPTPQLDGVVATQSSICLLSAEDNRLAYRGYDVAE
                     LAEKASYEETAYLLLAGKLPTRHELVRFVQELRARHKLPRRVQRVLRDAGPGADPMSL
                     LRTAVSASGLEPVTPAADIPIPAAALDLMALMPTLVAAVYRTGRGERTLTPRRSLGLA
                     GNLLFLLTGEKPARELARSVEIGLSLRADNELNPSTFAARVAAATKADLHGCITAALA
                     ALAGPLHGGHALAVYRLLEEIGSADRVAQVVDGRLATGKSFPGFGHPVYRGEDPRTAA
                     MQRAAETAAGLAGDRTWLDLAMAVENRVRAATGKHPNVDFYLAPLYRAAGIPPELFSA
                     IFAVARIPGWLAHVLEQQRQEGLIRPRAAYVGPARQEYVPIRRR"
BASE COUNT          481 a          893 c          898 g          474 t
ORIGIN      
        1 gcccgatcgc cggctgctcg cacttcttcg atcagcgctt cgtcgttgcc catgagcccc
       61 gacgccgtgt gtcccagtgc gatcacaatc gcgccggtaa gcgtggcgat gtcgatcacg
      121 gccgcaggct gataccgtcg agcgtacgag agtgcgtcgc acagaatcaa gcgaccctcg
      181 gcgttggtgt tgatcatctc gatggtcttg ccgaaatggc tcgtgatcac gtcgcccggt
      241 ttcacggccg tgccggaggg catgttctcg gtggagggaa tgagtcccac cacatgcact
      301 ttgggcttga gccggcccag catctcgaac gtccccagca ccgcggcggc gcctgacatg
      361 tcgaacttca tgtcttccat attctgcgcc ggcttgatcg agatgccgcc ggtatcgaag
      421 gtgacgccct tgccgaccag cacgaccggc ttggcatcgg accccttgta ctcgagggca
      481 atgaacctcg gctcttctgc gcttccctgc gcgatcgaga gcagagcgcc catcttttcg
      541 cggacgatcg ccgccttgtc gagcacggtt accgcgaatc catgccgctg agccagctcc
      601 tcagcggtct ttcccagaaa cgtgggcgtg cacacgttgc cgggaagcac ctgaagtcct
      661 cgcgtaaagg tctggcccgc gccgatcgcg gcgccgatct gatggcctgc ctcgacctcc
      721 gccgtcgctt cgggcgccaa aatggtgaac cgttcgagca cgggcttggg gtcttcgggc
      781 ggcttcttca tctcggtgaa gatccacgcg ccgtgcgcga gcccctcggc cgcgaattga
      841 cccacgtcgc gccatgagag cgggccacgt ccctcgggag cgagatagaa tgtgcccgac
      901 ggggcaccca ggcttcgggc ccgcttggcg ataatcgccg cggcgcggcg aacgtcgggc
      961 gcagcggtcc cctcgatttt gccgaggcct acgaggacga tgcgggcggc ggggccggtg
     1021 ggataaagaa cagtggtttc gtcgcgcttg cccgtgaagt cgccggcggc atagagccgg
     1081 gccagtgctc cgccagtagc acgatcgatt tcgcccagcg accccggaag gcccgtaccc
     1141 tgaggcacgg cgataccgag cagcggagtc tcgacagtgg ccggcggttt ggccgcgacg
     1201 gtggtctcga aaggcatgag tgtcccggtc tgcttaaaga aggttagcga cggcgaatgg
     1261 gaacgtactc ctgccgcgct ggtccgacgt aggctgctct gggacggatc agcccctcct
     1321 gacgctgctg ctccaacacg tgggccagcc acccgggaat tcgggcaacg gcgaagatgg
     1381 cagagaacaa ctcgggtgga atcccggcgg cccgataaag aggagcgaga tagaagtcca
     1441 cgttgggatg ctttccggtg gccgcgcgga cccggttttc aacggccatc gccagatcga
     1501 gccaggtgcg atcgccggcc aagccggcgg ccgtctccgc tgcacgctgc atggctgctg
     1561 tgcgcggatc ctcaccccgg tataccggat gcccgaagcc ggggaagctc tttcccgtag
     1621 ccagccggcc gtccaccacc tgcgcgacac ggtcggcgga tccaatctcc tcgagcagcc
     1681 gatagaccgc gagcgcatga ccaccgtgta gtggaccagc cagggcggcc agtgccgctg
     1741 ttatacagcc gtgcagatcc gccttcgtcg cagcagccac gcgcgcggcg aaggtcgacg
     1801 ggttgagctc attgtcggcc cgaaggctca acccaatctc aaccgaccgg gccagctcac
     1861 gcgccggctt ctcacccgtg agaagaaaga ggaggttgcc ggccagcccc aggcttcggc
     1921 ggggcgtcag cgtccgctcg ccgcgccctg tccgatacac ggccgccacg agggtcggca
     1981 tcagagccat caagtcgagc gctgcggccg gaatcggaat gtctgccgcc ggggtgaccg
     2041 gttccaaccc ggacgcgctc acggccgtcc gaagaaggct catgggatct gcgccgggcc
     2101 ccgcgtcccg caacacccgc tgtactcgcc ggggcagctt gtgccgggcc cgcagctcct
     2161 gcacgaaccg cacgagctca tgccgggttg gcaatttccc cgcgagcagc aaataggcgg
     2221 tctcttcata gctcgccttc tcagccagct ccgctacgtc ataaccccga taggcaagcc
     2281 ggttgtcctc ggcggagagg aggcagatgg aggactgggt agcaacgaca ccgtcgagtt
     2341 gaggggtggg aaaagcagca gacccaacgg ggctggtcat gagcacagtg tggcggggtt
     2401 gggacggcgc ggaagctaat acgccggttg attggcgggc aatgttcaag aacagcgtaa
     2461 acggtgaacg gtaaacggtg aaccggcggc tccggtgagc ggaacccccc gtttagcgtt
     2521 caccgttcac gctgtttgta gtcaactcct cccaccggct ccgccacccg ctcatgcctg
     2581 tggtccgctt accggtgccc ggaggcccgt tttcgcattt gcgcccccta cgcagtcgct
     2641 cgcgcgcgag ttccagtaaa cgagtgaatg ccgatcgagt ctggtcggct gggtgaggtc
     2701 ttcccgcgag ttgttggaag gaacgatgaa acgaggagca ggtgcg
//
LOCUS       DASRJQ010000324         2959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2648050, whole genome shotgun sequence.
ACCESSION   DASRJQ010000324 DASRJQ010000000
VERSION     DASRJQ010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2648050"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VFB89_07185"
     CDS             complement(<1..661)
                     /locus_tag="VFB89_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HZM27122.1"
                     /translation="MPSPQDLLAELHRRQAEAEQGGGSDRIAQQHAKGKLTARERLDL
                     LLDEGTFVELDRFVTHRATDFGLAEQQFPGDGVVTGYGRIDGRLVYVYSQDFTVFGGS
                     LSETHAQKICKVMDLALRNGAPVIGLSDSGGARIQEGVASLGGYADIFLRNTLVSGVV
                     PQISAVLGPCAGGAVYSPAITDFVFMVRGVSYMFVTGPSVVKTVTHEEVSFEDLGGAD
                     VH"
     gene            complement(789..2495)
                     /locus_tag="VFB89_07190"
     CDS             complement(789..2495)
                     /locus_tag="VFB89_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HZM27123.1"
                     /translation="MANLTTTPESFSTPTGLRVREDLIDESYSSEDYEKMLSMYEGTM
                     ASIAEGEIVKSKVLRVTDNAVILDVGFKSEGSVPLDEFKDIPKEGDEVEVFLEHLEDQ
                     EGAVVLSKKKADFMRVWEKIRIAHESDQPVEGTLMKKIKGGVVVNVMGVDAFLPGSQI
                     ALRRVPNIDELLGQSYEFKIIKLNKRRRNIVVSRRVILETERASKREHLKKELEVGEV
                     RKGIVKNITDFGAFIDLGGVDGLLHITDMSYGRVSHPTELVQIGTEVEVKVLDIDWQR
                     ERISLGMKQLQNYPWKDVAGKYPVGTRVQGKVVSITNYGAFVELEPGIEGLVHISEMS
                     WTRNVRHPSKIVSIGETIEAVVLKVDETEEKISLGMKQTEQDPWMVLPNKYPVGTRIQ
                     GKVRNLTSFGAFVELEPGIDGLIHISDMSWTKRVQHPSEVVKKGDSVDVVVLNIDAEN
                     KRISLGLKQAEEDPWLRIGESYPVGTNMRAKVVRLMDKGVVVDLGNDLEGFVPMSQLG
                     TEQVVENPADVVKEGQPLEVQVLEVDPIHHRIVVAVTGYPDEPFEAPPPRVIPPDDEI
                     VE"
     gene            complement(2659..>2959)
                     /locus_tag="VFB89_07195"
     CDS             complement(2659..>2959)
                     /locus_tag="VFB89_07195"
                     /inference="COORDINATES: protein motif:HMM:NF014416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27124.1"
                     /translation="LGEILNRAVNETLPRTVLTGGTALATLAALAIFGGEVVRPFAVI
                     MFFGIFTGTFSSIFIASPVLAAIHRRWPASSAKSQVPVVKATGATGRTPVSPASA"
BASE COUNT          531 a          979 c          915 g          534 t
ORIGIN      
        1 catggacgtc ggcgccgccc agatcctcga agctcacctc ctcgtgggtc accgtcttca
       61 ccacgctcgg cccggtgacg aacatgtagc tcaccccccg aaccatgaac acgaaatcgg
      121 tgatggccgg gctgtacacc gccccgccgg cacagggacc cagcactgcc gaaatctgcg
      181 ggacgacccc tgagaccagt gtgttccgca ggaaaatatc ggcgtacccg ccgagcgagg
      241 cgacgccttc ctgaatccga gcgcccccgg agtcggagag gccgatgacc ggggcaccat
      301 tgcggagggc gaggtccatc accttgcaga tcttctgcgc gtgggtctcc gacagcgatc
      361 ccccgaagac cgtgaagtcc tgcgagtaca cgtacaccag acgcccgtcg atccgcccgt
      421 acccggtgac gacgccatcg ccggggaact gctgctccgc cagaccgaag tcggtcgcgc
      481 gatgcgtgac gaatcgatcc agctcgacga acgtcccctc gtccagcagc aggtccaacc
      541 gctcccgcgc ggtgagcttt cccttggcat gttgctgggc gatccggtcg gagccgcctc
      601 cctgctctgc ttcggcctgg cggcggtgca attcggcgag gaggtcctga ggagagggca
      661 tgggggagaa gataggcggc gggacggctg gacggctaga cggctcgacg actgctgaac
      721 gcaaaaacgg ctggaaaccg aagttcccag ccgtccagcc gtccagtacc cagccgtcct
      781 gccgtcacct actcgacaat ttcatcatcg ggaggaatca cccgcggcgg cggagcctcg
      841 aacggctcgt cgggatatcc ggtcaccgcc accacgatgc ggtggtgaat gggatcgacc
      901 tcgagcacct gcacctcgag cggctggccc tctttcacca catccgcggg attctccacc
      961 acctgctcgg tcccgagctg ggacatcggc acgaagccct cgagatcgtt gccgaggtcc
     1021 acgacgactc ccttgtccat gagtcgcacg accttcgccc gcatgttggt gccgacgggg
     1081 tagctctcgc cgatccggag ccagggatct tcctcggcct gcttgaggcc cagcgagatg
     1141 cgcttgtttt ccgcgtcgat gttgagcacc acgacgtcga cactgtcacc cttcttcacc
     1201 acttcggacg gatgctggac ccgcttggtc catgacatgt cggagatgtg gatcagcccg
     1261 tcgatgcccg gctcgagctc gacgaaggcc ccgaagctgg tgaggttgcg gaccttcccc
     1321 tggatgcggg tgccgacggg atacttgttc ggaagcacca tccagggatc ctgctcggtc
     1381 tgcttcatgc caagggagat cttctcctcg gtctcgtcga ccttgagcac caccgcttcg
     1441 atggtctccc cgatcgagac gatcttcgac ggatgccgca cgttccgggt ccagctcatc
     1501 tcggagatgt ggaccagtcc ctcgatgccc ggctccagct ccacgaacgc gccgtaattg
     1561 gtgatcgaaa ccaccttgcc ctggacccgg gtgcccaccg ggtacttccc ggcgacgtcc
     1621 ttccagggat agttctgcag ctgcttcatg ccgagcgaaa tccgctcccg ctgccagtcg
     1681 atgtcgagca ccttcacttc gacctcggtg ccgatctgga ccagctcggt cggatgcgac
     1741 acccggccgt aggacatgtc ggtgatgtgg agcaggccgt cgacaccgcc gagatcgatg
     1801 aacgccccga aatcggtgat gttcttgacg atccccttcc gcacctcccc gacctcgagc
     1861 tccttcttca ggtgctcgcg cttgctggcc cgctcggtct cgaggatgac ccggcggctg
     1921 accacgatgt tccggcgccg cttgttgagc ttgatgatct tgaactcgta gctctggccc
     1981 agcagctcgt cgatgttggg aacccggcgg agcgcgatct gggagccggg aaggaaggcg
     2041 tccacgccca tcacgttcac caccacgccg cccttgatct tcttcatgag agtgccctcg
     2101 accggctggt cgctttcgtg cgcgatccgg attttttccc agacccgcat gaagtcggct
     2161 ttcttcttcg agaggacgac cgcgccctcc tggtcctcga gatgctcgag gaagacctcg
     2221 acctcgtcgc cctccttggg gatgtccttg aactcgtcga gcggaaccga gccctcggac
     2281 ttgaatccca catccagaat caccgcattg tcggtgaccc ggagcacttt ggacttgacg
     2341 atctcgcctt cggcgatgga cgccatggtg ccctcgtaca tcgagagcat cttctcgtag
     2401 tcttccgacg aataactctc gtcgatgaga tcttcgcgga cccgaaggcc cgtgggcgtg
     2461 gagaatgatt cgggcgtggt ggtgaggttg gccatgacgg aaggatcgct ttcggaccgg
     2521 ggccacgagc gccgccgggc tggaggtttg gtccccgtgc cgaagtgcac aggggagcct
     2581 tgcaaaatag acgcaaagac gcaaagacgc aaagacgcgc agacacaacg acgcaaagac
     2641 gcaaagtggg gctgttggtc atgcggatgc cggggatacg ggggtgcggc cggtggcccc
     2701 cgtcgccttg acgacgggaa cctgcgactt cgcggagctc gccggccagc gccggtgaat
     2761 ggccgccagc accggcgaag cgatgaagat cgacgagaag gtgccggtga agatcccgaa
     2821 gaacatgatc acggcaaagg ggcgaaccac ctccccgccg aagatcgcga gggccgcgag
     2881 ggtcgccagc gcggtgccgc cggtcaggac ggtccggggc agcgtctcgt tcacggcgcg
     2941 gttgagaatc tccccgagc
//
LOCUS       DASRJQ010000325        10214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3240704, whole genome shotgun sequence.
ACCESSION   DASRJQ010000325 DASRJQ010000000
VERSION     DASRJQ010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 10214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10214
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3240704"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1955)
                     /locus_tag="VFB89_07200"
     CDS             complement(<1..1955)
                     /locus_tag="VFB89_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZM27125.1"
                     /translation="MGTLPPIEVVGSIRPQANPSIGSGVPARITILSGREVDAYEPKL
                     ISDVLQQQAGISVYDDLGSSYKLTLSSRGFYASPVVGVPQGVSVFLDGVRMNEVDASQ
                     VNFDLLPMEHIKRIEILQGNGSLLGRNSLGGAVNLVTARGEGPLGGSVELSGGSFDSF
                     RGEGNISGLTRGGIDYYAGGMYNREDGWRQATDAREYNGFFNVGKLGQESGIRFQALF
                     AKSNAQTAGSLPESVFDVKPDSNFSPGDFEDLWQIQGSVAGYAQLGRGRGSFITFFRH
                     HEAERFNVNQPNDPNSFGTASNNSFGYTLDYRWATPVGSNAALSLRGGIDGSVNRAEI
                     ELFVDSTKFGGSRVPTTNARSPVWDVAPFVLADLTVGRATFSAGARYDHVEIPFEDLQ
                     DPSLDTTGVYNQVNPRVGVNVDLGSGLSAYGSWGQSFRAPAVIENACSDPENACPLPF
                     ALGDDPPLKPVKASTFEGGLTYASNRIYAGVSAYYTDVSNDIFVTPNENAPEGSTIAG
                     YFINLDKTRRKGIEVSTRYVWPAGHSVYLNYAHTLATFESPAEIFSPLEDAGFLNDVQ
                     PGDEFPLVPRDQIKGGVEVHPVRPVTLGLEGRFVGPQWLRGDEANQASKLPSYFVADA
                     RVGFEMAQWEVVGIVTNLFNRHYANFGA"
     gene            complement(2121..2717)
                     /locus_tag="VFB89_07205"
     CDS             complement(2121..2717)
                     /locus_tag="VFB89_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27126.1"
                     /translation="MKRKRDIPEHLRELDAALSRIRFYPRASLGPEVIGRLRRGERLK
                     GVGSPRISRSTLWAIAAALVLIVTGGFWVHRSGREVTVDHCCFDFDGGGRADDGVLVV
                     ASHGDQVHRIAVYEDRDGSHSFTPGDVIRIDRGRHVAVDGVTAGGLVTARHCCSDLDG
                     GGPPDDGLVLLSIPPDKVTLVALYESRPGPSQTRLTLR"
     gene            complement(2714..3265)
                     /locus_tag="VFB89_07210"
     CDS             complement(2714..3265)
                     /locus_tag="VFB89_07210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZM27127.1"
                     /translation="MTPPTVSNVGKTGSGAAANAEAVCGEWYESHGAAVYNYFRFHVA
                     DTDAAEDLTADTFLHVVRAIDCFDPNKGSPKTWILTVARNVLMDWRRRARLRQYVPIG
                     TLHDLAVEAPSPEERLLREEEVARLMNGIAALAPADRELIGLRYGSELDTAEMAAVLG
                     IREGAVRSRLWRAMSRLREELTS"
     gene            complement(3324..4523)
                     /locus_tag="VFB89_07215"
     CDS             complement(3324..4523)
                     /locus_tag="VFB89_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZM27128.1"
                     /translation="MATATVKSRFTGVDFYAMDSLLAEEERAVRDTIRAWVDEQLMPI
                     IGECYVEGRFPRELIPGLAELGVLGANLPEEYGCAGLNSVAYGLIMQELERGDSGVRS
                     FASVQGGLVMYPIFAFGSDEQKNHWLPRLASAQEIGCFGLTEPDFGSNPAGMITTARE
                     TRDGWVLNGTKMWITNGSQASVAIVWAKTGAVEDPRSIRGFIVPTSTPGFTATDQKGK
                     LSLRASDTSELHLEDVRLPADAILPRSGGVKSPLMCLSQARYGIAWGSVGAAMACYDE
                     AVSYAKSRIMFGKPIGQTQIQQVRLVEMLTEITKAQLLVLQLGRLKDAGTMTPEQVSL
                     AKRNNVNMACECAREARRLLGANGILAEYHSMRHLANLESVYTYEGTHDMHSLILGQS
                     ITGLSAF"
     gene            complement(4539..5285)
                     /locus_tag="VFB89_07220"
     CDS             complement(4539..5285)
                     /locus_tag="VFB89_07220"
                     /inference="COORDINATES: protein motif:HMM:NF021759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HZM27129.1"
                     /translation="MTSLPLLAGPDEVLANRLRLLGLRQVSGVRTHTNRTVMVSLNER
                     QKLRLHRGYVFAPDRVLAAIVRFLNPQAPRTERRRAEQVFLAFPVELYAPRPRERAGV
                     ERPRPGDVALLHRLRTLHRELNRTHFGGALGEIPIRLSGRMRTRLGEVSVDLRTGRPI
                     EIALSRRHLAEHPWPEVEHTMLHEMVHQWQAETGRSIDHGRTFRQKAREVGVLPAARR
                     AVARAGGPSGRSVRSAARETSELPVRKYHR"
     gene            complement(5285..6142)
                     /locus_tag="VFB89_07225"
     CDS             complement(5285..6142)
                     /locus_tag="VFB89_07225"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZM27130.1"
                     /translation="MTDISLVHLSDLHFGGLADLPQAEALEAFVPTLPAQAIVVSGDL
                     SQRARHGEFQRAKVFVQRMQEHAPTLVIPGNHDIEWWKSPLGIRGRRVKYAKYLRYFR
                     ELTPVLELPGAMIAGALSSYGVSLGSLTWNLNDIAVKGHLPRSETNRVKKIFARAPAD
                     AARVLVIHHNILPGAISRRMGLANWRPAQRRLLATGADVVLCGHDHQEGAGQVDGGVT
                     VSTSGTPSLRSRSGRPSMFNLVQIESSVVHVQHYRWEAADRRFVPSDRFSFARRVTPE
                     PVVSSAGGV"
     gene            complement(6139..7593)
                     /locus_tag="VFB89_07230"
     CDS             complement(6139..7593)
                     /locus_tag="VFB89_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27131.1"
                     /translation="MMRASALPNTGPRRFRGGKLVVAFVMAWSAFAPASARAQKEKGG
                     DNSRPPTSLTVYNDGRVFARRVLAIAVPKGETTQRVQPGQLDPGSLLALDPDVTILDA
                     RYDSVSSEQNALRRSVGRKFTFQSQSYPPVPIMATLLAVDPERWRLDDGTVLFQRPGL
                     IRWPDDLFAFEPSLELRVRAAQSKPALALGYFTTGAFWRASYQVILGGSDARVRGVAV
                     ITSQTLRVDSAEIQLLAGTIARPQGPQPRPYEMAEMVGAARKAGVAAGVGTEERVEEA
                     HIYTLAGRWSLRPGSTSLLPLFDESPTSVVREYVVRREPVYGILPENDAIEEPPVQVD
                     YTLARPRKTGFGDRPLPAGIARLFRTDSDGRLQVVGEAALEHTAPGKELVLKAGNAFD
                     LSARRRQTDFITARDSSAAGLRTRAEAAYEVTLANATDSAATVIVQESRAGEWSVLTS
                     SVPAEKVSSTLTRFRLKVPARGQAALTYRIRAIW"
     gene            complement(7590..7760)
                     /locus_tag="VFB89_07235"
     CDS             complement(7590..7760)
                     /locus_tag="VFB89_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27132.1"
                     /translation="MSRWVAFGATALMAVYLALRIPAHSPMRLYAIVAVLATYIIVRR
                     IATRIAMEFLPR"
     gene            complement(7858..8874)
                     /locus_tag="VFB89_07240"
     CDS             complement(7858..8874)
                     /locus_tag="VFB89_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HZM27133.1"
                     /translation="MTYDVTLIPGDGIGPEITAQTVKVLEATGLRFTWDEELAGMAAV
                     DATGTPLPDATVESIRKNGLALKGPLTTPVGTGFRSVNVGLRKEFELFANVRPAKTIV
                     PGGRFENIDIVLVRENLEGLYIGVEHFVRVGDDPRAVGESMAIVTRTGCERIVRYAFE
                     YAIRHRRKRVTIVHKANILKMVSGLFLEVGRTIANEFQGRVESNDIIVDNCAMQLVMR
                     PEQFDVMVTTNMFGDILSDEVSGLVGGLGLAPGANIGTKAAIFEAVHGSAPDIAGKGI
                     ANPSAQMLAAAMMLDHMGELDRAQRIRQALEHVIVVQKVRTRDLGGNASTQEFGNAVA
                     KRVA"
     gene            complement(8871..9557)
                     /locus_tag="VFB89_07245"
     CDS             complement(8871..9557)
                     /locus_tag="VFB89_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27134.1"
                     /translation="MRITLPDPVPITARAVVVRLHFAVVLALVAGMPAAGAAQDADKP
                     PRQLATAGLAGQSVALLPITLALADSTVRHDSTVTSLGDRTHVLHWADSIIGEGFLGN
                     APEVTWVLPPRLRQLARRNPGMIPEPDQMGQAILRSPKIGKIPDPLQSSLRSLVNVTG
                     GRYAMVPAALALSRDSTGSLRADLALALADARAGTVVWRSNGVGRGGTPSEAVAAAVT
                     SVLPSSALGP"
     gene            complement(9590..>10214)
                     /locus_tag="VFB89_07250"
     CDS             complement(9590..>10214)
                     /locus_tag="VFB89_07250"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZM27135.1"
                     /translation="QALLTETALMSMGVGVYASRCDVRDRNAVDLFIRDTKTRLGGIH
                     VLVNNAGIASDGALWRLTEEAWREVLETNVTGAFNCIRAVAPFFRHQHYGKIVNVSAH
                     QADRPGFGVSNYAASKAALLGLTRAAAVELGPSNVNVNAVAPGFVRTERMMMLPKEVI
                     ERAQKSSILGRVAEPDDVAHVVSFLCSEGARHITGQTITVDGGLSLE"
BASE COUNT         1818 a         3378 c         3231 g         1787 t
ORIGIN      
        1 gcgccgaagt tggcgtagtg ccggttgaac aggttggtga cgatcccgac cacctcccac
       61 tgagccatct cgaaccctac ccgcgcgtcc gccacgaaat agctgggcag cttcgacgcc
      121 tggttggcct catcgcctcg cagccactga gggcccacga accggccttc gagaccgagc
      181 gtgacgggac ggaccgggtg cacttcgacg ccacctttga tctgatcccg gggaaccaac
      241 ggaaactcat cccccggttg cacgtcgttg aggaaccccg cgtcttcgag ggggctgaag
      301 atctcagcag gcgactcgaa cgtcgccaag gtgtgcgcgt agttgaggta cacggaatgt
      361 cccgccggcc atacgtaacg agtgctgacc tcgatgccct tccggcgagt cttgtcgaga
      421 ttgatgaagt agccggcgat ggtgcttccc tcaggcgcgt tttcgttcgg ggtcacgaag
      481 atgtcgttcg acacgtcggt gtagtacgcg gaaaccccgg cgtagatccg gttggacgcg
      541 taggtaaggc ctccctcgaa ggtgctcgcc ttcaccggct tgagcggcgg atcgtcgccc
      601 agcgcaaacg gaagcggaca ggcgttctcg ggatcggaac aggcattctc gatgacggcc
      661 ggcgcgcgga agctttgtcc ccaggagccg tacgccgaaa gcccgctgcc caggtccacg
      721 ttcacgccga ctcgaggatt gacctggttg tagacgccgg tcgtatcgag actcggatcc
      781 tgcaggtctt cgaatgggat ctcgacatgg tcataccgag cccccgcgga aaacgttgct
      841 cgtccaacgg tgagatctgc gaggacaaat ggcgcgacat cccagaccgg cgagcgagcg
      901 ttggtcgttg gtacccggct gcctccgaac tttgtcgaat cgacaaagag ctcgatctcc
      961 gcgcggttca cacttccatc aatgcccccg cggaggctga gcgcagcgtt ggaacccacc
     1021 ggcgtggccc aacggtagtc cagcgtatag ccgaagctgt tgttgctcgc agtcccgaac
     1081 gagttcgggt cgttgggctg gttaacgttg aatcgctcag cctcatggtg ccggaagaac
     1141 gtgatgaacg agccccggcc ccggccgagc tgggcgtagc ctgctaccga gccctggatc
     1201 tgccacagat cctcgaagtc tcccggtgaa aagttcgagt ccggcttcac atcaaatact
     1261 gattcaggga gcgagccggc tgtttgggcg ttcgatttgg cgaagagtgc ctggaaccgg
     1321 attcccgatt cctggcccag cttgccgaca ttgaaaaagc cgttgtactc gcgggcgtcg
     1381 gtcgcctggc gccagccgtc ttcccggttg tacatgcccc cggcgtagta atcgatcccg
     1441 cccctggtga ggccggagat gttgccctcg ccccggaagc tgtcgaagct tccgcccgac
     1501 agctcgacgc tgcccccgag cggaccctca ccccgcgccg tgaccagatt gacagcaccg
     1561 ccgagcgagt tgcggccgag cagcgagcca ttgccctgca ggatctcgat gcgcttgatg
     1621 tgctccatgg ggaggaggtc aaagttgacc tgggatgcat ccacttcgtt catccggaca
     1681 ccgtcgagga agactgagac gccctgcggt actccaacga cgggggaggc atagaagccg
     1741 cgcgaggaga gagtcagctt gtatgagctg ccgaggtcat cgtagacgga gatgccggcc
     1801 tgctgctgta gcacatccga gatgagtttt ggctcgtagg cgtcgacttc ccgaccggaa
     1861 agaatcgtga tccgggcggg tacgcccgag ccgatggacg gattggcctg cggcctgatg
     1921 ctccccacga cctcgatcgg cggaagcgtg cccaccgagt cgcgccgggt cgtgtcaggc
     1981 gtggctcccg gagtcgatgg ctgctgcgct gcaagcggcg ccgcaaggaa gactgcagcc
     2041 gtgatccggc aagccaggcg cgtgccaagg gcaggaatca gtagaaaact cccggccaga
     2101 tgtggtgcgg ggtattccgg ctagcgcaac gttagccgag tttgcgatgg acccggccgg
     2161 ctctcgtaca acgcgaccag ggtcacctta tcgggcggaa tggagagcag caccaagcca
     2221 tcatccgggg gcccgccgcc gtccaggtcg ctgcagcagt ggcgcgcggt gacaaggccg
     2281 ccggcagtca ccccgtcgac ggcaacatgc cgaccccggt cgatccgaat cacatcccct
     2341 ggagtgaagc tgtgggagcc gtcgcgatcc tcgtaaacgg cgatccgatg cacttggtcc
     2401 ccgtgagaag ccaccacaag gaccccatcg tccgctcggc ctcccccgtc gaagtcgaag
     2461 cagcagtgat cgactgtgac ttcccggccg gatcgatgga cccagaaacc gcccgtaacg
     2521 atcagaacga gggcagctgc gatggcccag agggtcgacc ggctgatccg tggcgatccg
     2581 acacccttca accgttcgcc ccgacgcaac cggccgatta cctccggacc aagagaggcc
     2641 ctgggataga agcgaatccg ggacagcgcg gcatccaact cgcgcaggtg ctctgggatg
     2701 tcccgcttcc gcttcacgac gtaagttcct cacgcagtct gctcatcgcc cgccagagcc
     2761 gacttcgcac ggctccttcg cgtattccca ggaccgcagc catttctgcg gtgtccagct
     2821 cgctgccata acgcagcccg atcagctccc ggtcggccgg tgcgagtgcg gcaattccgt
     2881 tcatcagccg agcgacttcc tcttcccgaa gcaatcgttc ctcgggcgac ggcgcttcga
     2941 cggccagatc gtgcagcgtg ccgatcggta catactggcg aagccgggcc cttcgcctcc
     3001 aatccatcag cacattgcgc gcgaccgtca ggatccaggt cttgggtgat cccttgttgg
     3061 gatcgaaaca atcgatcgcg cgaaccacat ggagaaacgt atcggcggtc aggtcctctg
     3121 ccgcgtcagt atccgccacg tgaaaccgga agtagttgta caccgccgct ccgtgggact
     3181 cgtaccattc cccgcacacg gcttcggcgt ttgccgccgc cccgctgccc gtcttgccga
     3241 cgttgctgac tgtgggaggc gtcacttccc ttacctacgc acaaggagcg gttgcgtcac
     3301 cgatcggcat gatcgggctc gggctagaat gcgctcaacc cggtaatcga ctggcccagg
     3361 atgagcgagt gcatatcatg ggtgccttcg taggtataca ccgactcgag attggccaga
     3421 tgccgcatcg aatggtactc ggccagaatg ccgttggcgc cgagcaaccg gcgggcctcc
     3481 cgagcgcact cgcaggccat gttgacgttg ttccgtttgg ccagcgagac ctgctccggc
     3541 gtcatggtgc cggcgtcttt gagccggccc agctgaagga cgaggagctg cgccttggtg
     3601 atctccgtga gcatctccac caggcgcacc tgctggatct gggtctggcc gatcggcttg
     3661 ccgaacatga tgcggctctt cgcgtagctc accgcctcgt cgtagcatgc catcgcggcg
     3721 ccgaccgagc cccaggcaat accatagcgt gcctggctga gacacatgag cgggctcttg
     3781 acaccgccgg aacggggaag gatggcgtct gccggcaagc gtacgtcttc caggtgtagc
     3841 tcgctcgtgt ccgatgcccg cagcgacagc tttccctttt gatcggtggc ggtgaaccct
     3901 ggcgtgctgg tgggaacgat gaatccgcgg atcgacctgg ggtcctcgac cgcccccgtc
     3961 ttggcccaga cgattgccac cgatgcctgc gacccgttgg tgatccacat cttcgtaccg
     4021 ttcagcaccc atccgtcgcg cgtctcccgc gcggtggtga tcatgcccgc gggattggag
     4081 ccgaagtcag gctcggtcag gccgaaacag ccgatttcct gggccgacgc aagccgggga
     4141 agccagtggt tcttctgctc gtcgctcccg aacgcaaaga tgggatacat caccaagcca
     4201 ccctgcaccg aggcaaacga ccggactccg ctgtcgcccc gctccagctc ctgcatgatg
     4261 agcccgtagg ccacgctgtt gagccccgcg cagccatact cttccggcaa attggcgccc
     4321 aacacaccca gctcggccaa tccggggatc agctcgcgcg gaaagcgtcc ttccacgtag
     4381 cactcgccga tgatcggcat gagctgctcg tccacccagg cacggatggt atcgcgcacc
     4441 gcacgctcct cttccgccag gagcgaatcc atcgcgtaga agtcgacacc ggtgaatctg
     4501 gatttaactg tggccgtcgc catacgagtc tcggagacct agcggtgata cttccggacg
     4561 ggcagctcgg aggtctcgcg cgcagccgac cggacggacc ggccggacgg tccgccggcc
     4621 cgcgccactg cgcgacgggc cgcaggcagg acgccgactt cgcgggcctt ctgccggaag
     4681 gtacgcccgt ggtcgatgga tcgcccggtt tccgcctgcc actgatgcac catctcgtgc
     4741 agcatcgtgt gctcgacctc gggccaggga tgctcggcga gatggcgacg gctcaacgcg
     4801 atttcgatgg gtcggccggt gcgaaggtcc acactcacct cgcccagccg ggttcgcatc
     4861 cggccggaga ggcggatggg aatttcgccg agtgcgccgc cgaagtgagt tcgattgagc
     4921 tctcggtgaa gcgtacgcag ccggtgtagc agggcgacgt cgccggggcg cgggcgttcc
     4981 accccggccc gttcacgcgg tcttggcgca tagagctcga caggaaaggc caggaatacc
     5041 tgctccgccc gccgcctctc ggtacggggt gcttgggggt tgagaaagcg aacgatcgcg
     5101 gccagcaccc ggtccggggc gaagacgtat ccccggtgaa gccggagttt ctgtcgttcg
     5161 ttcaggctga ccatcactgt acgattggtg tgggtgcgaa ccccgctcac ctgtcgaagg
     5221 ccgagcagcc ggagccgatt cgccaggact tcgtccggcc ctgcgagcaa ggggagcgac
     5281 gtcattagac gcctcccgcg ctcgagacta caggctcggg tgtcacccga cgtgcaaagc
     5341 tgaaccgatc cgacggcacg aaccggcggt ccgccgcttc ccagcggtag tgttgcacgt
     5401 gcaccaccga cgactcgatc tggaccaggt tgaacatcga cggccggccg ctccgcgagc
     5461 ggaggcttgg cgtgccggac gtgctcacgg tcaccccgcc atctacctgt cctgccccct
     5521 cttgatggtc gtggccgcac agaacgacat cggcgccggt ggcgagcagt cggcgctgtg
     5581 ctggccgcca gttggccaga cccatccgcc gcgaaatagc ccccggtaga atgttgtggt
     5641 ggatgacgag cacgcgggct gcgtccgctg gcgcgcgagc gaagatcttc ttgacccggt
     5701 tggtttccga gcgcggcaaa tgacccttga cggcgatgtc gttcaggttc cacgtgaggg
     5761 agccgagcga cacaccgtag ctgctcaagg ctcccgcgat catcgccccc ggaagctcga
     5821 gcaccggggt gagctcgcga aagtagcgca ggtacttcgc atacttgacc cgtcgtccac
     5881 gaattccaag ggggctcttc caccattcga tgtcgtggtt gcccggaatg accagcgtag
     5941 gagcatgttc ctgcatgcgt tgcacgaata ccttggcccg ctggaactcg ccatgccgag
     6001 cgcgctgcga gagatcgccg gagaccacga ttgcctgggc cggcaacgtc ggcacgaatg
     6061 cctcgagcgc ttcggcctgg ggaagatcag ccaacccgcc gaaatgcaga tcggagagat
     6121 gaaccagcga gatgtcggtc accagatagc acggattcga taggtgagcg ctgcttgccc
     6181 gcgggccggc actttgaggc ggaaccgggt cagagtggac gacactttct cggcaggcac
     6241 cgaactggtg agtacggacc actcgcccgc gcggctctcc tggacgatga ccgttgccgc
     6301 agaatcggtc gcgttggcga gggtaacctc gtacgccgct tcagcgcggg ttcgcagccc
     6361 ggctgcacta ctatcccggg cagtgatgaa atctgtctga cggcgccgcg cgctcaagtc
     6421 gaaggcgttt ccggccttga gcacaagctc cttgcccgga gcggtgtgct ccagcgcggc
     6481 ttcgccgaca acttgcagcc gcccgtcgct gtcggttcga aagaggcgcg cgatgccggc
     6541 cggcaaaggc cggtcgccga aaccagtctt ccgtgggcgg gcgagggtgt agtccacttg
     6601 caccggtggc tcctcgatcg cgtcgttctc cggcaagatg ccatacaccg gctctcggcg
     6661 aaccacgtac tcgcgtacaa cgctcgtggg cgattcgtcg aacagaggga gcaggctggt
     6721 cgagcccggt cggagtgacc agcgtccggc cagggtgtaa atgtgagctt cctcgaccct
     6781 ctcttctgtt cctactcctg ccgctacgcc ggccttccgc gcggccccca ccatctcggc
     6841 catctcgtac ggtcggggtt gcggcccctg cggtcgagcg atggtgcccg cgagcagctg
     6901 aatctccgcg ctgtccaccc gaagcgtctg cgacgtaatc accgccacgc cccgcacccg
     6961 tgcatccgac ccaccaagga ttacctgata gctcgcccgc cagaaagcgc cggtcgtgaa
     7021 gtagcccaat gcgagcgccg gcttcgactg cgccgcgcga acccgaagct cgagcgacgg
     7081 ctcgaaggca aacaggtcat cgggccagcg aatgagaccc ggtcgttgga acagcacggt
     7141 accgtcgtcc agccgccaac gctccggatc gaccgcgagg agcgtcgcca tgatcgggac
     7201 tggagggtag ctctgactct ggaacgtgaa cttccgaccc actgatcgcc gcaaggcgtt
     7261 ctgctcgctg ctgacggagt catagcgggc gtcgagaatg gtgacgtccg gatcgagcgc
     7321 cagaagcgag cccggatcga gctgacccgg ctgaacccgc tgggtggtct cgcctttggg
     7381 cacggcgatc gccagcaccc gtcgcgcgaa cacccgcccg tcgttgtaca cggtgaggct
     7441 ggtgggcggg cggctgttgt ctccgccctt ctccttctgc gcgcgcgctg acgccggagc
     7501 gaaggccgac cacgccatca cgaacgcaac gacgagcttc ccgcccctga aacgtcgcgg
     7561 tcctgtgttc ggcagcgcac tcgctcgcat catctgggca ggaactccat tgctatccgg
     7621 gtagcgatcc gtcgcacgat gatgtacgtg gcgagcacgg cgacgatcgc gtagagccgc
     7681 atcggcgagt gcgccgggat tcgcaaagcc aggtacacgg ccatcagagc agtggccccg
     7741 aaagcgaccc acctcgacac ccgtcgcgcc cggccctccc gttcccagcg acattcgggg
     7801 cagagttccg tccaggccag ccccgggacc cgctttccgc acttggcaca cgggacctca
     7861 ggcaacccgc ttagcgacgg cattcccgaa ctcctgcgtg ctggcgttcc cgccgagatc
     7921 gcgggtacga accttctgca ccacgatgac gtgctcgagc gcctggcgga tgcgttgcgc
     7981 ccggtccagc tctcccatgt gatcgagcat catggcggcg gccagcatct gagccgacgg
     8041 attcgcgatg cccttccccg cgatgtccgg cgcgcttccg tggaccgcct cgaagattgc
     8101 agccttggtg ccgatgttgg cgcccggcgc gaggcccagc ccacccacca ggcccgatac
     8161 ttcgtcgctc aggatgtcgc cgaacatgtt cgtcgtcacc atcacgtcga actgctccgg
     8221 ccgcatcacc aactgcatgg cgcagttgtc cacgataatg tcgttcgatt ccaccctgcc
     8281 ctgaaactca ttcgcaatgg tgcggccgac ttcgagaaac aggccgctga ccatcttgag
     8341 gatattcgcc ttgtgcacga tggtgacacg cttccgtcga tggcggattg cgtattcgaa
     8401 tgcgtaccgc acgatgcgct cgcagcccgt ccgcgtgacg atcgccatcg attcgcccac
     8461 tgcgcgcgga tcgtcgccga cccgcacgaa atgctctact ccaatgtaga gaccttcaag
     8521 attctcgcgg accagaacga tatcgatgtt ctcgaagcgg ccgccgggca cgatcgtctt
     8581 ggcgggacga acatttgcga agagctcgaa ttccttgcga agccctacgt tcaccgatcg
     8641 gaacccggtt cccaccggcg tggtgagcgg gcctttgagg gccagcccgt tcttcctgat
     8701 gctctcgact gtggcgtcgg ggagcggggt tccggtcgcg tcgaccgccg ccatgcccgc
     8761 cagctcttcg tcccacgtga accggagccc tgtcgcctcg agcactttga ccgtttgagc
     8821 cgtgatctcg gggccgatgc cgtcgcccgg gatcaaggta acgtcgtagg tcatggcccg
     8881 agggcactgc tcggaagcac ggaagtgact gcggcggcga cggcctcact gggagtgccg
     8941 cccctgccga cgccgttcga gcgccagact acggtccccg cccgggcgtc cgcgagagcg
     9001 agcgccaaat cggcccgcaa gctgccggtg ctgtcgcgac tcagagcgag tgcagctggt
     9061 accatcgcat agcggccgcc ggtcacgttg accagactgc ggagcgagct ttgaagcggg
     9121 tccgggatct tgccgatctt cggcgaccgg agaatggcct ggcccatctg atcaggttcc
     9181 ggaatcattc ccgggttgcg acgagccagt tggcgaagcc tgggtggcag tacccaggtc
     9241 acctccggcg cgttccccag aaacccctcg ccaatgatcg agtcggccca gtggagaacg
     9301 tgggttcggt cacccagcga cgtcaccgtg ctgtcgtgtc ggaccgtgct gtcggcgagc
     9361 gccaacgtga tcgggagaag cgcgactgat tggcccgcga gaccggcggt cgcgagctga
     9421 cgcggcggct tgtctgcatc ctgcgccgcg ccggccgccg gcattccggc gacgagcgcg
     9481 agcacaactg caaaatggag gcgcacgacg acggctcgag cagtgatggg gaccggatcg
     9541 ggaagtgtga tgcgcaaaag ctaacgacgg gagggacgta agagaacgtc tactcgagcg
     9601 acagtcctcc atcgacagtg atggtctgac ccgtgatgtg cctcgcacct tccgagcaca
     9661 gaaacgagac cacgtgagcg acatcgtccg gctcagcgac gcgcccgaga atcgagctct
     9721 tctgcgcccg ctcgatcacc tctttcggca gcatcatcat ccgctcggtc cgaacgaacc
     9781 ccggcgcaac cgcgttgacg ttcacgttgg atggtcccag ctcgacggcg gccgcacggg
     9841 tgaggccgag cagcgccgct ttgctggccg cgtagttgga caccccgaac cccggccgat
     9901 cggcctgatg agcgctcacg ttgacgatct tgccgtagtg ctgatggcgg aagaacggcg
     9961 cgacggcccg gatacagttg aaggcgccgg tcacgttggt ctccaacact tcgcgccagg
    10021 cctcctcggt cagtcgccag agcgcaccat cgctcgcgat ccctgcgttg ttcaccagca
    10081 catggatgcc gccgaggcgg gtcttggtgt cccggatgaa aagatcgacc gcattccgat
    10141 cccgcacgtc acagcgggag gcatagaccc ctacgcccat ggacatgagc gcggtctcgg
    10201 tcagcagggc ctgc
//
LOCUS       DASRJQ010000326         2656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2450577, whole genome shotgun sequence.
ACCESSION   DASRJQ010000326 DASRJQ010000000
VERSION     DASRJQ010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2450577"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VFB89_07255"
     CDS             complement(<1..641)
                     /locus_tag="VFB89_07255"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27136.1"
                     /translation="MIGESGTVARTMMAVLAAALGGLAGTAAVRMLALRLGIVNQPNP
                     LVPQHTRPIAYLGGVGVALGVGSGLSIHALLGGATPGIEFVLPAVLYLVLGVFDDLRA
                     LEPGRKFLLQALIAALAVGLGFVAPLCGVPLLDAVASWLWIVTLVNAFNLTDVCDGLL
                     ASLSAVMFGALALLDPSAAPTALVISAASLGFLVLNRPPALVFLGDAGSHLLG"
     gene            complement(638..2011)
                     /locus_tag="VFB89_07260"
     CDS             complement(638..2011)
                     /locus_tag="VFB89_07260"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZM27137.1"
                     /translation="MPKKTDVLILGSGLTGLAAAWVLADRATILERDSRPGGLVRTER
                     FGDYWFDRVLHLLYFQDPDTERRVRGLLGNTLAPCPPVAWCETAFGTTRFPFQMNLRG
                     LDVDTRVACVRDLAEATFRPPAQPAANFEQHLLATFGRSMCEAFLFPYNRKMWKRPLD
                     TLAPSGFTWNITSPDLDQVLRGAFETQSAFKAYNSAGWYPRPPRGAALRGMEVVAHAL
                     AKEAHELRLGWEVEEVDLRRREVLARHAGGRERFGYREALLSTIPLPALVERCPDLPS
                     GLRSECARLHRNRVLSVAIRVRGPRPQDPGHWRYYADESLIFTRLVFLQEFDPWTGPD
                     DGWALLVEITERAEAPVTERDALLARVRADLERVAAIPPGCEIVGQNVMVIDPAYVVF
                     EPHGQSVVDSALHLLAQHGVTSLGRYGKWEYSSMASCMRDGLAWAEGLPGAATGSIST
                     ALAGVGA"
     gene            complement(2138..>2656)
                     /locus_tag="VFB89_07265"
     CDS             complement(2138..>2656)
                     /locus_tag="VFB89_07265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZM27138.1"
                     /translation="TRIRFLIVGRGHGLAELTHRAARLELPNLSFLPTQPRERLAEML
                     AAADVGLVTLDPKLAATSVPSKTFTIMAAGRPVLAAVHPDNEVAQMVVAAQCGVIAPP
                     DDPVAISAILRAWADDGSPLDAMGSRARSWAERFHGRGSAVQTHEDILASESRSARRQ
                     TGPDPLAETRPE"
BASE COUNT          473 a          910 c          905 g          368 t
ORIGIN      
        1 aagccgagca ggtggcttcc ggcgtcgccc aggaagacca gggcgggcgg acggttgagc
       61 accaggaacc cgaggctggc cgcgctgatc accagggcgg tgggtgctgc ggagggatcg
      121 agaagggcca gcgccccgaa catcacggcg ctcagggacg ccagaagacc gtcgcagacg
      181 tcggtcaggt tgaatgcatt gaccagggtg acgatccaca gccacgaggc caccgcatcc
      241 agtagcggca cgccgcacag cggcgcgaca aagcccaggc cgactgccaa ggcggcgatc
      301 agtgcttgaa ggaggaactt ccgccccggt tccagggccc tcagatcgtc gaacacgccg
      361 agcaccaggt acagaaccgc ggggagcacg aactcgatcc cgggagtcgc gccgccaagc
      421 agcgcgtgaa tcgacaggcc cgaacccact cccagcgcta cacccacgcc ccccaggtac
      481 gcgatcggcc gcgtgtgctg cggcaccaga gggttgggct ggttcacgat gcccaggcgc
      541 agggccagca tccgaacagc ggctgttccc gccaggccgc cgagcgcagc ggccaggacc
      601 gccatcatgg ttcgggccac ggtcccagac tctccgatca cgcgcccact ccggcgaggg
      661 ccgtggaaat cgagcccgtc gctgcccccg ggagaccctc ggcccaggcc aggccgtcgc
      721 gcatgcacga cgccatcgag gagtattccc atttgccgta acgccccagg ctggtcacgc
      781 cgtgctgggc cagcaagtgc agggcggaat cgaccaccga ctggccatgg ggttcgaaga
      841 ccacgtaggc cggatcgatg accatcacat tctggcccac gatctcgcac cccggcggaa
      901 tcgccgcgac ccgctccagg tcggcacgca cccgcgccag caaagcgtcg cgttcggtga
      961 ccggggcctc cgcgcgctcg gtaatttcca ccagcagcgc ccagccgtcg tcgggcccgg
     1021 tccatggatc gaactcctgg agaaacacca ggcgagtgaa gatcagcgac tcgtcggcgt
     1081 agtaccgcca atgaccaggg tcctgagggc gcggcccacg cacgcgaatg gccaccgaca
     1141 gcacgcgatt gcgatggagt cgtgcgcact cgctgcgcag cccggacggg aggtcggggc
     1201 agcgctccac cagcgccgga agcgggatcg tggagagcag cgcctcacgg tagccgaacc
     1261 gctcacgccc gcccgcgtgc cgcgccaaca cctcacgccg ccgtaggtcc acctcctcga
     1321 cctcccagcc cagtcgcagc tcgtgcgcct ccttggccag ggcatgcgcc accacctcca
     1381 ttcctcgaag cgctgcgccg cggggggggc gcgggtacca gccggcgctg ttgtaggcct
     1441 tgaacgccga ttgggtttca aacgctccgc gcagcacctg atcgagatcc ggggaggtga
     1501 tattccaggt gaaaccactg ggggccaggg tgtcgagcgg gcgcttccac atcttgcggt
     1561 tgtacgggaa cagaaaggcc tcgcacatcg atcggccgaa agtcgcgagc agatgttgct
     1621 cgaagttcgc cgcaggctgc gccgggggcc ggaaggtggc ctcggccaga tcgcgcacgc
     1681 acgccacgcg ggtgtcgacg tcgaggccgc gcaggttcat ctggaagggg aagcgcgtgg
     1741 ttccgaaagc ggtctcacac catgcgacgg gcgggcaggg agcgagggta ttgcccagga
     1801 ggccgcgcac acgccgctcc gtgtcgggat cctggaagta cagcaggtgg aggacgcgat
     1861 cgaaccagta gtcgccgaat cgctcggtgc gaaccagtcc cccggggcgc gagtcgcgct
     1921 ccagaatcgt ggcccgatcc gccagcaccc acgcggcggc caggcccgtg agacccgatc
     1981 cgaggatcag gacatcggtt tttttgggca tcggctcctt ccgaccccga gagatggcct
     2041 gagagcccgg ccatcccccg aacatcttcc gggtcgtcgt atcgcgggtt ccgcgagtcg
     2101 aaaacccttc gccgggcatc ctgcccagcg agttgcgtta ctccggtcgc gtctccgcca
     2161 gcgggtccgg tcccgtttgc cttcgtgcgc tccgggactc gctcgcgagg atgtcctcgt
     2221 gagtctggac ggcggaacct cgaccgtgaa accgctcggc ccagcttcgg gctctcgacc
     2281 ccatcgcgtc aagtggagat ccgtcgtcgg cccaggcccg caaaatcgcc gaaatggcga
     2341 ccggatcatc gggaggggcg atgacgccgc actgcgcggc gaccaccatt tgagcgactt
     2401 cgttgtccgg atggacggca gcgagcaccg gacggcccgc cgccatgatg gtgaaggtct
     2461 tgctcggtac gctggtggcc gcgagcttgg ggtccagcgt gaccaggcca acgtccgctg
     2521 cggccagcat ctccgccagt cgttcgcgag gctgggtggg caggaacgag aggttgggaa
     2581 gctcgagccg cgcggcacgg tgggtgagct cggcgagacc atggccgcgt ccgacgatca
     2641 ggaagcggat gcgcgt
//
LOCUS       DASRJQ010000327         7059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2942993, whole genome shotgun sequence.
ACCESSION   DASRJQ010000327 DASRJQ010000000
VERSION     DASRJQ010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7059
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2942993"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VFB89_07270"
     CDS             <1..439
                     /locus_tag="VFB89_07270"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HZM27139.1"
                     /translation="EAFGRRIEHMGPVGAGHAMKAVNNALLAAHLQAFGEGFAALAKA
                     GVPARKALEVLNASSGRSFVSESLVPDRVLTGRWPQTFRLALLEKDVRIALGLLDETG
                     VGGPLLHLVGQLFRQARAILGEAADHVEIIRLIEQEAGVEIRG"
     gene            510..971
                     /locus_tag="VFB89_07275"
     CDS             510..971
                     /locus_tag="VFB89_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27140.1"
                     /translation="MSEDWGSIRQEAAQVLLAEGTAFEGSLHLLLRLQYPAGPETPLE
                     MLNRADPFFALTGATNGVVFVPKAQTAVVTCPHQAPADPERLSAAKLIALHVTVVGGR
                     EFQGRAAIELPPGRSRALDYVNGPGTFFALWNDEVTHYINKLLVRTIRPLD"
     gene            981..2393
                     /locus_tag="VFB89_07280"
     CDS             981..2393
                     /locus_tag="VFB89_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HZM27141.1"
                     /translation="MARLDQLIRVLHEQRAESLQLAVGKPAVLLRDGTTRPVTKEALS
                     DAQIAALLREIAPAEAAGGIGSPEAVRFGYKAPSGSVQVEARPGDVTLRPAGAGSRSE
                     KEVRELRTAMEALLRRLVESSASDLHLRTGEPPVLRQNGELVRLELEPVSAERLEGML
                     TSIMGSKELNEFRETNDTDWAYELEALARFRCNAGRDRRGPMAVFRTIPGRVMTADEM
                     GLSREVQNLCYLTKGLVLVTGPTGSGKSTTLAALVDLINRTRTDHILTIEDPIEFVHQ
                     SKKCLVTQRQVGLHTRSFKQALRAALREDPDIVLVGEMRDLETVSIALETAETGHLVF
                     GTLHTTTAASTIDRIIDQFPADRQSQVRVMLSESLRGVIAQMLCRKIGGGRVAAREIL
                     LTIPAVSNLIREGKTFQIPSIMQTNRKAGMVTLNDALIELVDQGLVEPKEAYMKAVEK
                     AGFAQALKAKRHDVVFLSEA"
     gene            2390..3184
                     /locus_tag="VFB89_07285"
     CDS             2390..3184
                     /locus_tag="VFB89_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HZM27142.1"
                     /translation="MSSPGQSKPILVLVAPQDIVNVASVVRIAKNFAIDRIRLVNPEM
                     ELDAYRIEGIAHNTADLVARITVHQSLPDALADCIYAAVLTGRERAAKRSRLRPREAA
                     LELVARVEDGPVAIVAGREDSGLTNDELDCCDALVTISTDPAHPSLNLAQAVAIMAYE
                     SWLARGGDRLPFKRPRRTAAAAPNALLETLFADWTRALWAIDFFKTRQPANVMRSFRE
                     VVFRAGLDAREASLLRAMGIEISRFLAREGLVAAATPPEAPPSEEP"
     gene            3187..3666
                     /locus_tag="VFB89_07290"
     CDS             3187..3666
                     /locus_tag="VFB89_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HZM27143.1"
                     /translation="MGRSPAYWAANQYRAADAPPKPSAPYRDLPEPLGDRFRLARTGL
                     LAIRGVGEQVRFMGSPWGWTWEFGIGSRKLCWLHPITNGVSATFTLTDDEESKALGFA
                     RLAEVVRQAIREGQRTGPVKWCWVSLNDRRQIDGFLNLVRRKADWIAAAATRRRRVV"
     gene            3849..4445
                     /locus_tag="VFB89_07295"
     CDS             3849..4445
                     /locus_tag="VFB89_07295"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HZM27144.1"
                     /translation="MRKPVLITLGAGAAAIGLAALAITSGRTGAQAPKSVADSLKAPV
                     LAAGDTSALRGPRQPIFFRHDIHAGQFKIQCQYCHYSVAVSSEPGIPSMQTCMGCHNV
                     IAGTDSSHKVEIKKLRDASSQKKPIEWTRVHFVARHVHFPHMRHIQVLGPNACQTCHG
                     DVARMPQVYKVNNVNNMGFCITCHEQRGVTRDCAVCHY"
     gene            4538..>7059
                     /locus_tag="VFB89_07300"
     CDS             4538..>7059
                     /locus_tag="VFB89_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZM27145.1"
                     /translation="MSNTDNGSSSIDRRRFLTVLGVSGAGATMLTGCSTDKVQKLIPY
                     LVQSEDQVPGVATWYASTCTECAAGCGLHVKTREGRAIKLEGNPDHPINRGKLCSRGQ
                     ASLQGLYNPGRVKGPLARGAGGEWQEITWDDGIQRFAAKLSAASGKVAVISGAGRGTF
                     SDLLADWTGALGGKVTRYQPFDHEPLRTANRAVFGRDEVPAHDFAKAQYIVSFGADFL
                     ETWLAPIENQRGFAESHGFHDGKMARHVAFAPRMSLTGQNADEWHAVTPGSEAALALA
                     LANLLVSEHGADAGGIGASLSRFTPEMAAKETGVDVATIRRIGKELAEAKPSLVVAGG
                     VADQHRGAVELCAAVNILNYVAGNIGQTVLFGGDLQAGDGAAGIDAVGAALDGGHVAV
                     LLVHEANPLYALPASTGFAARFKKAGFKVSTSPYFDETAAQCDLILPNHHSLERWDDV
                     HPRAGVAGLMQPVMEPVFRTMATGDVLLKVAQKAGGALARFNAPSFEAQLKNRWAELA
                     KQQGQADAEGFWRAAVQRGGLYGDRPAAAEVKMAAGVEATYTAPAFDGSGEFYFAPHV
                     SSFLYDGRGTNKPWLLENPDPVTKLTWHSWVEVHPDTAARLDVRNGEIVRLVSPQGAI
                     EAPVCVYPGIHPQVLSVPLGFGHTEYGAYAKGRGVNALDLLGAKSGQAFVAYLSTKVA
                     VEKTRKYQKLASTEGNPRQLGRHIAEAMPVALAAKGLTPAEADRAEGHPEEEVNTERE
                     LEALKGWRAAVQQRNQRGNYAGDHPQWGMVIDLAKCTGCSACVTACYAENNIPTVGEE
                     EIHRGREMTWMRIERYWEGQTTPTSAPEPRFVPMLCQHCANAP"
BASE COUNT         1276 a         2210 c         2335 g         1238 t
ORIGIN      
        1 cgaggccttc ggccggcgga tcgaacatat gggacccgta ggcgccgggc acgccatgaa
       61 ggccgtcaac aacgctctcc tcgccgcgca tttgcaggca ttcggcgaag ggttcgctgc
      121 cttggcaaag gcgggtgtgc ccgctcgtaa agcactcgag gtcctcaatg cctcgagtgg
      181 ccggtcgttc gtgagcgagt ctctggttcc cgaccgggtg cttaccggcc gctggcccca
      241 gacgttccgc ctggctctgc tggaaaaaga cgttcgaatc gcgctcggtt tgctggacga
      301 gacgggggtt ggcggaccgc tgcttcatct ggtgggtcag ttgttccgcc aggcccgggc
      361 aatcctgggc gaggcggcgg accacgtcga gattatccgt ctgattgaac aggaggccgg
      421 cgtcgaaata cgcgggtgag ccggcccttg ccgcagtgat gggcgtctga cgagtttccc
      481 ttcgctatgt ccgttccgac aaccattgga tgagcgagga ctgggggtcc attcggcagg
      541 aggcagccca ggtgctgctt gccgagggta ccgcgttcga gggcagcctg catctcctgt
      601 tgaggctgca atacccggcg ggacccgaga cgccgctcga gatgctcaat cgggccgatc
      661 cgtttttcgc gctcactggc gctacgaacg gcgtggtatt cgtaccgaaa gcgcaaacgg
      721 cggtggtaac ctgtccccat caggctccgg cggatcccga gcggctctcc gcggccaagc
      781 tcatcgccct ccacgttaca gtcgtgggcg gccgggaatt ccagggcagg gccgcaatcg
      841 agttgccgcc cggacgaagc cgggcgctcg attacgtgaa cggtcccggc accttcttcg
      901 cactatggaa cgatgaagtc acccattaca tcaacaagct cctggtgcgg accatccgcc
      961 ccctggacta acgaggcagc gtggcccgac tcgatcaatt gatccgggtg ctacacgaac
     1021 agcgcgcgga gtcactccag ctggcagtgg ggaagccggc tgtgctgctg cgggacggaa
     1081 cgacgcgccc ggtcaccaag gaggcgctca gcgatgcaca gatcgctgcg ctgcttcgcg
     1141 aaatcgctcc cgcggaggct gccggaggca tcggctcgcc cgaggcggta cggttcgggt
     1201 acaaggcccc ttcgggcagc gtgcaagtcg aggcgcggcc gggcgacgtg acccttcgac
     1261 cggcgggggc cggaagccga agcgaaaaag aagtgcgcga gctgcggact gccatggagg
     1321 ctttgctccg gcggctggtc gagtcgagcg cctcggacct ccatctcagg acgggagagc
     1381 cgccggttct ccggcagaac ggtgagctgg tgcggctcga actggagccg gtcagcgcag
     1441 agcggctgga agggatgctg accagcatca tgggctcgaa ggagctgaac gagttccgcg
     1501 agaccaacga caccgactgg gcctacgagc tggaggccct ggctcgattc cgatgcaacg
     1561 ccggccgcga ccggcgcggt ccgatggcgg tgttccggac catcccgggc cgggtcatga
     1621 ctgccgacga gatgggcctg agccgggagg tgcagaacct ctgctacctc accaaagggc
     1681 tggtgctggt gacaggacca acgggctcgg gcaagtccac cacgctcgcc gcgctggtcg
     1741 atctgatcaa ccggacccga accgatcaca tcctcacgat cgaagacccg atcgagttcg
     1801 tccatcaaag caagaaatgt ctggtcaccc aacgtcaggt ggggctccat acacgctcgt
     1861 tcaagcaggc actgcgggcg gccctccggg aggatccgga tatcgtgctg gtgggtgaaa
     1921 tgcgggatct cgagaccgtg tcgatcgcgt tggaaacggc cgagaccgga cacctggtct
     1981 tcggcacgtt gcatacgacg acggctgcca gcacgatcga tcgcatcatc gaccagttcc
     2041 cggccgaccg tcagtcgcaa gtgcgcgtca tgctctcgga atcgctgcgc ggagtcattg
     2101 ctcagatgct ctgtcgtaaa atcggtggtg gacgggtagc cgcccgcgaa atcctgctta
     2161 ccattccggc cgtctccaac ctgatccgcg agggaaagac gttccagatc ccctccatca
     2221 tgcaaaccaa ccgcaaggcc ggcatggtca cgctcaatga tgcgttgatc gagctggtgg
     2281 atcaggggtt ggtcgagccg aaggaagcct acatgaaagc ggtcgaaaag gccggcttcg
     2341 cgcaggcact caaggccaaa cgccacgacg ttgtcttcct cagcgaggca tgagctctcc
     2401 cggccagtca aagccgattc tcgtgctggt ggcgccccag gacatcgtca acgtcgccag
     2461 cgtggtccga atcgcgaaga acttcgccat tgaccggatt cggctggtga atccggaaat
     2521 ggagctcgac gcctaccgga tcgaaggcat tgcgcacaac acggcggatc tggtcgcccg
     2581 catcaccgtc caccaatcgc tgcccgacgc attggcagac tgcatctacg cggctgtcct
     2641 gaccggacgt gagcgcgccg ccaagcgaag tcgactccgg ccgcgggagg cagcgttgga
     2701 attggtggcg cgcgtggagg acgggccggt cgcgatcgtc gccggtcggg aggattccgg
     2761 gcttaccaac gatgagctgg attgctgcga cgccctggtc accatatcga ccgaccccgc
     2821 ccacccgtcg ctcaacctcg cccaggcagt cgcaatcatg gcgtatgaaa gctggctcgc
     2881 ccggggaggc gatcggctac cattcaagcg accgcgccga actgccgcgg cggctcccaa
     2941 tgcgctgctg gagacgctgt tcgccgactg gacccgagcg ctctgggcca tcgacttctt
     3001 caagaccagg caacccgcca acgtgatgcg ttcgtttcga gaggtcgtgt ttcgggcggg
     3061 gctcgacgcc cgcgaggcct cgctgctgcg ggctatggga attgagattt cccgcttcct
     3121 ggcgcgggag ggtctcgttg ccgcagcgac gcctcccgag gctcctccga gcgaggagcc
     3181 ctgagcgtgg gacgatctcc ggcatactgg gctgccaatc agtatcgagc ggccgacgcc
     3241 ccacctaaac catccgcgcc gtatcgcgat ctcccagagc cgctgggtga ccgatttcgc
     3301 cttgcgcgta ccggcctcct cgccattcga ggcgtaggcg agcaggtgcg gttcatggga
     3361 tcgccctggg gctggacctg ggaattcggg atcggttcgc ggaaactctg ctggctccac
     3421 ccgatcacca acggcgtgag cgccaccttc actctcacgg acgacgagga gtcgaaggcg
     3481 ctcggattcg ctcgccttgc cgaagtggtg cgtcaggcga tccgtgaagg acaacgaacc
     3541 gggccggtca agtggtgttg ggtatccctc aacgaccgtc ggcaaattga tggtttcctg
     3601 aacctggtgc ggcgcaaagc ggactggatt gcggcggcgg cgactcggcg ccgtcgagtg
     3661 gtttaggcgc gtgaggatca ggagcgatcc cgttgtggag tttcgcagtg gctgactata
     3721 cttcctcccg ctcgagggca ggctcccccg acgtgctgtc tctcgcgtac aagtgttgac
     3781 gtgctgaccg ctttcaacag gtgtggcggt gcaggttgaa gccggccggc gtttgactgg
     3841 tgacgacgat gcggaagccg gtactcatca ctctgggagc gggtgcggca gccatcgggc
     3901 tcgcggcgtt agccatcacg tcagggcgaa ccggcgcgca ggcgcccaag tcggtggcgg
     3961 actctctcaa agcccccgtg ctcgctgccg gtgatacgtc agcgctgcga gggccccgcc
     4021 agccgatctt cttccgccac gacattcacg ccggccaatt caagatccaa tgccagtact
     4081 gccactactc ggtggcagtg tcgtccgagc cgggcattcc gtccatgcag acctgcatgg
     4141 gctgtcacaa cgtcatcgcg gggaccgaca gcagtcacaa ggtcgagatc aagaagctgc
     4201 gggatgcctc gtcacagaaa aaaccgattg agtggacccg ggtgcatttc gtcgcgcggc
     4261 acgtccactt tccccacatg cggcacatcc aggtcttggg accgaacgcg tgtcagacct
     4321 gtcacggcga cgtcgcgcgc atgccgcagg tctacaaggt gaacaacgta aacaacatgg
     4381 gtttttgcat tacctgtcat gagcagcgcg gggtcaccag agactgcgcg gtatgccatt
     4441 actaggagct atcagctatc agctatcaga ccctcagcga tcagcgcttg caacgttagg
     4501 ctgatagctg atgactgata gctgctagcc cgacaccatg tccaatactg acaacgggtc
     4561 gtcttccatc gatcgccgcc ggtttctgac tgtcctcgga gtatccgggg cgggcgctac
     4621 gatgctgacc ggctgttcga ccgataaagt ccagaagctc attccgtatc tggtccagtc
     4681 ggaagatcag gtgcccggcg tggccacgtg gtacgcgagc acgtgcacgg agtgcgctgc
     4741 gggctgtggg cttcacgtca aaacccgcga agggcgcgcg atcaagctcg agggaaatcc
     4801 ggaccacccc atcaatcggg gtaagctctg ttctcggggc caggcctcgc tgcagggact
     4861 ctacaatccc gggcgggtca aaggtccgct ggcgcgcggc gccgggggcg agtggcagga
     4921 aatcacctgg gacgatggca ttcagcggtt cgccgccaaa ctttcggcgg cgagcggcaa
     4981 ggtggcggtc atttcggggg cgggtcgcgg gacattttct gatctgctgg ctgactggac
     5041 cggcgcgctt ggtggcaagg tgacgcgcta ccagcccttt gatcacgagc cgctccgcac
     5101 agccaaccgc gccgttttcg gccgcgacga ggtgccggcg cacgatttcg cgaaggcgca
     5161 gtacatcgtc tcgttcggcg ctgattttct cgagacctgg ctcgcaccga tcgagaatca
     5221 gcgtggcttc gccgagtcgc atggttttca cgacggcaag atggcgcggc acgtagcatt
     5281 cgcaccccgc atgtcgctca cggggcagaa cgccgacgag tggcacgctg tcacgcccgg
     5341 ctccgaagcg gcgctcgcgc tggcccttgc caacctgctc gtttcggaac acggggccga
     5401 tgccggcgga atcggagcct ctctgagtcg attcacaccc gagatggcgg ccaaggagac
     5461 cggcgtcgac gtcgccacga ttcgacggat cggaaaggag ttggccgagg ccaagccgag
     5521 cctggtggtt gccggcggcg tggccgatca gcaccgcggt gccgtcgagc tctgcgccgc
     5581 ggtcaacatc ctgaactacg tcgcggggaa catcggccag accgtgctgt tcggcggcga
     5641 tcttcaagcc ggcgatggcg cagccggcat tgacgcagtc ggggcggcgc tcgacggcgg
     5701 tcacgtggcc gtcctcctgg ttcacgaagc caatccactg tacgcgcttc ccgcgtccac
     5761 cggtttcgcg gcgcggttca agaaggccgg cttcaaggtc tcgacctccc cctatttcga
     5821 cgagaccgcg gcgcagtgcg acctcatcct gccaaaccac cactcgctcg agcgatggga
     5881 tgatgtgcat cccagggctg gagtagcggg cctgatgcag cccgtgatgg agccggtttt
     5941 ccgcaccatg gcgaccggag acgtactgct caaggttgcg cagaaggcgg gtggtgcgtt
     6001 ggctcgcttc aacgcgccga gcttcgaagc gcagctcaag aaccgatggg ccgagctggc
     6061 caagcaacag ggccaggcgg acgctgaggg gttctggcgc gctgcggtgc agcggggtgg
     6121 gctctatggc gaccgtcccg ccgcggccga agtgaagatg gcagcgggcg tcgaggccac
     6181 ttacactgcg cccgccttcg atggcagcgg cgagttctac ttcgcgccgc acgtctcttc
     6241 attcctgtac gatggtcgcg gcaccaacaa gccgtggctg ctcgaaaacc cggatccggt
     6301 caccaagctt acttggcatt cgtgggttga ggtccacccc gatacagctg cgcggctgga
     6361 tgtgcgcaac ggcgagattg tccggctggt ctcgccccag ggcgccatcg aggcgccggt
     6421 ctgcgtgtac cccggcattc atcctcaggt gctgtccgta cctcttggct tcgggcatac
     6481 cgaatacggc gcctacgcca aaggtcgcgg ggtcaatgcg ctggatctct tgggtgccaa
     6541 gagcgggcag gccttcgtcg cttacctgtc gacgaaggtt gcggtcgaaa agactcgcaa
     6601 gtaccagaag ctcgcctcga ctgagggcaa cccccgtcag ttgggccggc acattgctga
     6661 ggcgatgccg gttgcgctgg cagccaaggg tctcaccccc gcggaggctg atagggcgga
     6721 gggtcatccc gaggaagagg tcaacaccga gcgcgagttg gaggcgctca agggctggag
     6781 agcggcggtc cagcaacgga accaaagggg caactatgct ggtgatcacc cgcaatgggg
     6841 gatggttatc gatttggcga agtgcaccgg ctgttcggcc tgcgtcactg cctgctatgc
     6901 cgagaacaac atcccgaccg tcggtgagga ggagatccat cgcgggcggg agatgacctg
     6961 gatgcggatc gagcggtact gggagggcca gaccacgcct accagcgcgc ccgaaccaag
     7021 gttcgtgccg atgctctgcc agcactgcgc caacgcgcc
//
LOCUS       DASRJQ010000328         5049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1650089, whole genome shotgun sequence.
ACCESSION   DASRJQ010000328 DASRJQ010000000
VERSION     DASRJQ010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1650089"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VFB89_07305"
     CDS             complement(<1..406)
                     /locus_tag="VFB89_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZM27146.1"
                     /translation="MRNQRGFTLIELLIAMVLMVIVSGALYRLLTGTQRLSRSQVERA
                     DMQANMRAGALVLPAELRELGYDSVPLGAVNSDIIAMASDSVVIRAIRASGVICKLAA
                     NTITIDTTRNYAALRTLRGGAGQDSLMIFNESD"
     gene            complement(412..792)
                     /locus_tag="VFB89_07310"
     CDS             complement(412..792)
                     /locus_tag="VFB89_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HZM27147.1"
                     /translation="MSNERGFTIVEVMVAMLVLIIGATALVGSSGIVSRQIGRGRSIS
                     RATQVATQRLETLRRAANRRTSVGGNRCQHASFASGTATTLGMSESWGVATAGTVRSV
                     VDTVTYGRTSGTSRVILRTLIACY"
     gene            complement(789..1241)
                     /locus_tag="VFB89_07315"
     CDS             complement(789..1241)
                     /locus_tag="VFB89_07315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HZM27148.1"
                     /translation="MTNRRGFTLIETLIVLGVAAVLLGLSWPKIKTLMLKANVRSARS
                     AVIGYYQQGRMRALQSGRPTTIWFSGNRMWVTATPRRNAGTGTYDTLGYVYDFKQRYG
                     VTVAASPDTTVSVNARGLGISANSMTLALTRSGVRDSVVINQIGRVTK"
     gene            complement(1451..2815)
                     /locus_tag="VFB89_07320"
     CDS             complement(1451..2815)
                     /locus_tag="VFB89_07320"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZM27149.1"
                     /translation="MRLLIVDDDADLRQSLSLLLREAGYEVVAEADPEEGLRRALAEE
                     FDLILCDVKMPRMDGLAFLRRYRGERGAALIIMMSAYGAEDAALEAMREGAYDYIRKP
                     FRPDEVTMVVRKAEERERLRREVETLRSSIGADAARDLVVAESKAMRDLLELASRVAR
                     HNTTVLVTGESGTGKEVLAKAIHRMSPRHEASFTAINCAAIPEQLLESELFGHTKGAF
                     TGATADRAGLFETAHQGTLLLDEIGDLPLSLQAKLLRVLEEGEIRRVGGRDGKKVDVR
                     VIAATAKPLDEAVERGEFRPDLYYRLDVVRLHIPPLRERREDIPALLTHFARQAAQRL
                     GHPVSITPQAFTLLSEHSWPGNVRELRNAVERAAVLSGTKALDWGDFALGNGTKANGH
                     GPVPANGSFDLRSQVEEVERALIQRALEASKGNRREAAHLLGISLRTLFYKLRRVQQH
                     TNGN"
     gene            complement(2796..3992)
                     /locus_tag="VFB89_07325"
     CDS             complement(2796..3992)
                     /locus_tag="VFB89_07325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZM27150.1"
                     /translation="MPQPRRSLRTELLVNLGFVTSSAVILVGLTTVVLAGGDLDATLR
                     PLLVLWLASTVVFVLFGAYVLRRLVLTPLQRLSAEADLLAAGGLEPTRPGYESPELAH
                     LANRYRAMADSLIDAQSQMIRVEKLAGIGSLAAGVAHEVRNPLGALSSYVDVLRRRGA
                     DREVTDAMRGAIERIERTVQSLLTYAKPSVGQGATDLNSAVRSSLEFLGAQGLLKNQR
                     LTVELEPGLPNVRGDGHLLEQVVVNLVVNACQASPGGALVVGTARQAFEPRYRVATRA
                     SDPWRPETSAARAERDPLWSSRPRRADVPAGTPGVLLYVADDGPGIPESDRERVFDPF
                     YTTKDPGEGTGLGLAIVARTVHDAGGTVWVDRAREGGAVFKVFLPLAWQSVPSVVEPG
                     YAPAHR"
     gene            complement(4144..4551)
                     /locus_tag="VFB89_07330"
     CDS             complement(4144..4551)
                     /locus_tag="VFB89_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZM27151.1"
                     /translation="MSNRKGFTLIELLIVVVIIGILAAIAIPKFANTKQKAVRSSMIS
                     DLRNLATAQEAFYADSTRYAGSYSSTLGSNSVNFSPSQNNVMAITTGGSGSYWSATMT
                     NTGYNSGSSTCGIFVGTTATVSGSTAEGVPGCQ"
BASE COUNT          847 a         1690 c         1605 g          907 t
ORIGIN      
        1 ggtcggactc gttgaagatc atgagcgagt cctggccggc gccgcccctg agcgtacgca
       61 acgcggcgta gttccgcgtg gtatcgatgg tgatggtgtt cgccgccagc ttgcagatga
      121 cgccgctggc gcggatggcg cgaatcacca cactgtcgga cgccatggcg atgatgtcgc
      181 tattgacggc cccaaggggg accgagtcat agcccagctc ccgcagctcc gccggcagca
      241 ccagggcacc ggcgcgcatg ttggcttgca tatcggctcg ctcgacctgc gagcgggaca
      301 gccgttgcgt gccggtcagc agccgataca gggcgccgct cacgatcacc atgaggacca
      361 tggcgatgag caactcgatc agggtaaatc cgcgctgatt ccgcataggt gtcagtagca
      421 ggcgatgagg gtacgtagaa tgacccggct ggttccgctg gtccggccgt aggtcacggt
      481 gtcaacgacg ctccgaaccg ttccagcagt agcgactccc cagctctcac tcatgccaag
      541 cgtggtggcg gtaccgctgg cgaaactggc gtgctggcag cggttcccac ccacgctggt
      601 gcgtcggttg gccgcgcgcc gcagcgtctc gagccgttgg gtggcgacct gcgttgccct
      661 actgatggac ctgccccggc cgatctgccg gctgacgatg ccggacgagc cgaccagcgc
      721 ggtggcgcca ataatgagca ccagcatggc caccatcacc tcgacaatcg tgaatccgcg
      781 ctcgttgctc acttggtcac tctcccgatc tgattgatca caacggaatc gcgaacgccc
      841 gagcgggtga gcgccagcgt catggagttc gccgagatgc ccaggcctcg ggcgttcacg
      901 ctcacggtgg tatcgggact ggcggcaaca gtcacgccgt accgttgctt gaagtcgtac
      961 acgtacccca gcgtgtcgta ggtgccggtc cccgcatttc ggcgcggcgt ggcagtcacc
     1021 cacatgcggt tgcccgaaaa ccagatggtg gtcgggcgtc cgctttggag cgcgcgcatt
     1081 ctcccttgct ggtaataccc gatcacggcg ctccgtgcgc tccgtacgtt ggccttgagc
     1141 atgagcgtct tgatcttggg ccagctgagg cccaggagga cggcggccac ccccagcacg
     1201 atgagtgtct cgatcagggt aaaaccgcga cggttggtca ctgccaagag agcctcccgg
     1261 ggctggtcac gcgtcggccc gcgaactgtt cggtgtaaaa gcagggcctg taccagaacg
     1321 taagttgttg atgggcaacg attcgatacg atttgtcagg ctggtttcac gcagggatct
     1381 gcgcggagtg caacggatgg cgctctcact aggcagccgc ctacaaataa cgcgggctgc
     1441 tccgtgcgac tcagttgccg ttggtgtgct gctgaacccg gcgcagcttg tagaacagcg
     1501 tccgcaggct gatccccagc agatgggccg cctcgcgccg attgcctttc gaggcctcca
     1561 gcgcccgctg gatcagcgcg cgctcaacct cttctacctg cgagcggaga tcgaacgagc
     1621 cgttcgcagg caccggcccg tggccgttcg ccttggtgcc gttcccgagc gcgaagtcgc
     1681 cccagtcgag cgccttggta ccgctcagta cggccgcccg ctccaccgca ttccggagct
     1741 cgcggacgtt ccccggccag gaatgctcgg acagcagggt aaacgcctgc ggcgtgatgc
     1801 tcacgggatg ccccaggcgc tgcgcagcct gtcgtgcgaa gtgggtgagc agcgcgggga
     1861 tgtcctcacg tcgttcacga agtggcggga tgtgcagacg caccacatcg aggcggtaat
     1921 acaggtcggg gcgaaattca ccgcgctcca ccgcctcatc cagcggcttg gccgtcgcgg
     1981 cgatgacgcg cacgtcgacc ttcttaccgt cccggccgcc cacccgacgg atctccccct
     2041 cctcgagcac gcgcagcagc ttggcctgca ggctcagggg gagatcgccg atctcatcca
     2101 gcaggagcgt gccctggtgc gccgtctcga acagcccggc ccggtccgcc gttgcgccgg
     2161 tgaaggcgcc tttagtgtgg ccgaacaact cggactcgag cagctgctcc ggaatcgcgg
     2221 cgcagttgat cgcggtgaag ctcgcctcgt gccgcggact catccggtga atggccttgg
     2281 cgaggacttc cttgccggtg ccgctttcgc cggtgacgag aaccgtggtg ttgtgccggg
     2341 cgacccggct cgccagctcg agcaggtctc gcatggcctt gctctccgcc acaaccaggt
     2401 ctcgcgcggc gtccgctcca atgctcgagc gcagcgtttc cacctcgcgg cgcagtcgct
     2461 cccgctcctc cgctttccgc acgaccatgg tgacttcgtc agggcggaac ggcttccgga
     2521 tgtagtcgta ggccccttcc cgcatcgctt cgagggcggc gtcttctgcg ccatacgcgc
     2581 tcatcatgat gatcagcgcc gcacctcgct cgcctcggta ccggcgcaga aaggccaatc
     2641 cgtccatccg cggcatcttc acgtcgcaga ggataagatc gaactcctcc gcgagcgcac
     2701 ggcggagccc ttcttcggga tcagcctcgg ccaccacctc gtaccctgcc tcgcggagca
     2761 ggagactcag ggattggcga aggtcggcgt cgtcgtcaac gatgagcagg cgcatagccg
     2821 ggttccacga cagagggcac agactgccag gcgagaggca ggaaaacctt gaagacggcc
     2881 ccaccttcgc gcgcacgatc tacccagacg gtgccacccg cgtcgtgcac ggtacgggcg
     2941 acaattgcga gacccaggcc ggttccctct cccggatctt tggtggtgta aaacgggtcg
     3001 aacacgcgtt cccggtcgct ttccggtatg ccggggccgt cgtcggcgac gtacagcagc
     3061 acacccggag tacctgccgg cacatccgct cggcgcgggc gacttgacca gagcgggtcg
     3121 cgctcggctc tcgctgcgct cgtttccggc ctccagggat cgctcgcccg ggtggcgacc
     3181 cgatagcgcg gctcaaatgc ctgccgcgcg gttcctacga ccagcgctcc ccccggagat
     3241 gcctggcacg cgttgaccac caggttcacc actacctgct ccaggaggtg accgtcgcct
     3301 cgaacgttcg gcagacccgg ctccagctcg accgtgagcc gctgattctt gagcagaccc
     3361 tgcgcgccga gaaactcgag gctggatcgc acggcgctgt tgagatccgt tgcgccctga
     3421 ccgacactcg gcttggcata cgtaagcaga ctttgtaccg tccgctcgat tcgctcgatg
     3481 gcgccccgca tggcgtcggt gacttcccga tccgctcccc gccggcgaag cacatccacg
     3541 tagctgctca gtgcacccag cggattcctg acttcgtgcg ccactcccgc ggccaggctt
     3601 ccgatgccgg ccagcttttc gacgcgaatc atctggctct gcgcgtcgat caggctgtcg
     3661 gccatggccc gataccgatt cgccaggtgc gccagctcgg ggctctcata gcccgggcgc
     3721 gtcggctcga gtccacccgc ggccagcaga tccgcctcgg ctgagagacg ctgaagcggg
     3781 gtgagcacca ggcgccgcaa cacgtaggcg ccgaagagca cgaagaccac cgtgctggcc
     3841 agccacagta ccaggagtgg ccgcagcgtc gcgtcgagat ctccgcccgc gagcacgacc
     3901 gtcgtcagcc cgaccaggat gaccgccgac gaggttacga atccgagatt caccagcagc
     3961 tcggttcgga gcgatcgacg gggctgcggc atttccggtc gatcctaagc gcgcgcgcgc
     4021 cggtccttgg cggacggcgc gcagaaagtc aggttgtgtc tctcgatcac gttgagttgc
     4081 ttggctacag cgaggggcat gggcagcgga tgccacccat gcccccgccg tgaaccgttc
     4141 agcttactga cagcccggca cgccctcggc cgtggaaccg gacaccgtcg cagtggtgcc
     4201 gacgaagatg ccgcaggtgg acgaaccgct gttatagccg gtgttggtca tcgtggccga
     4261 ccagtacgag ccgctgccgc ccgtggtgat cgccatgacg ttgttctggc tgggcgagaa
     4321 gttcaccgag ttcgagccca gggtgctgct gtagctgccg gcgtaccggg tcgagtcggc
     4381 gtagaacgcc tcctgcgcgg tggccaggtt acgcaggtcc gagatcatgc tggaccggac
     4441 cgccttctgc ttggtgttcg cgaacttcgg aatggcgatc gccgccagaa tgccgatgat
     4501 cacgaccacg atcagcagct cgatgagggt gaaaccctta cggttcgaca tttttcctcc
     4561 aagtgtggtc gcctgcggct gaacgccggg agccgagaat cgggtgtctc cggcacgtcg
     4621 ggaggggcaa cccgtatgcc accgttaggc cgcgacgcct caactctcgg agccgacgct
     4681 atttacggac gtagttgagg cttcgccaag agtcccgttt cgagagccgg agccgaattt
     4741 tgcaagatgt tccgagcgct caagccgctc accacaggag tgcggaatct ggcatcaata
     4801 tttggaacga ttctcgccat tccggtggct gaacgaccgc cgctggacac catcatcgtt
     4861 gcaattgtct aatggagttc agcttgctcg ctgcaccatc atccacttac ccctcgccaa
     4921 atccggtatc cctgggacgg atgcaagatt tgtctgcaga tcccgtgtct gatccgcgca
     4981 cgagcatggc tcggcgggcg ctggactggg cgcccgccct agccctggtc ctcctcgcgc
     5041 tggcggcca
//
LOCUS       DASRJQ010000329         2027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3670517, whole genome shotgun sequence.
ACCESSION   DASRJQ010000329 DASRJQ010000000
VERSION     DASRJQ010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3670517"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1751
                     /gene="purL"
                     /locus_tag="VFB89_07335"
     CDS             <1..1751
                     /gene="purL"
                     /locus_tag="VFB89_07335"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF002290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HZM27152.1"
                     /translation="APGYTGNPLVNAMCVGLLRERDLMRARAHGVGNVLLVVGSRTGR
                     DGIHGASFASEELSEESEARRPQVQVGDPFTEKLLLEATLELITSDLVVAVQDMGAAG
                     LTSSSAEMAARGGVGVEIDTGLVPTREDGMTPYEILLSESQERMLVVAEPQRLREIQA
                     VCEKWELTATPIGRVTDDGIFRVRHHNRVVAAIPGQPLVDDCPIYHPEAHEADEAISR
                     RAARPSSTAQPDPEKALEALLDTPNLASKRWVYEQYDSTVQSATVIGPGGDAGVLQVP
                     GTEFGLAITVDCNARRVALDPYEGGKAVVAEAARNIACTGALPIGITDCLNFGNPEKP
                     EVFFQFREACRGISDACRAFETPVTGGNVSFYNESPTGAVDPTPVVGMVGLLERVAGR
                     VGSHWVEPGDAILILGATQGELGGSAYWAEVLGFVGGQPARVDLAAERRLQLLLVAAA
                     RVGLLRSAHDCTEGGLAVALAEAAIGGPYAKRSLGARLDLDRYAEGLPAERVLFGEDD
                     ARAVVSCAPGAVKALTALAGEHGVPAFAAGTVSAPEGWLELRITGRMLTWDTIALRRI
                     YYDAIPRRMAHAEQDR"
     gene            1765..>2027
                     /locus_tag="VFB89_07340"
     CDS             1765..>2027
                     /locus_tag="VFB89_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HZM27153.1"
                     /translation="MCGIIGVSGLTDAAKLAYLGLYALQHRGQESAGIVAIDPEGRPR
                     SYRGMGLVSETFDDNILATLPGDIAVGHTRYSTTGSTVLANAQP"
BASE COUNT          340 a          638 c          718 g          331 t
ORIGIN      
        1 tcgcacccgg ctacaccggc aatccgctgg tcaacgcgat gtgtgtcgga ctgctgcggg
       61 agcgggacct gatgcgggca cgggcgcacg gcgtcggcaa tgtgctgctg gtcgtcggat
      121 cgcgtaccgg tcgggacgga attcatggcg ccagcttcgc ctcggaagaa ctgtcggagg
      181 agagcgaagc ccgccggccg caggtccagg tgggcgatcc cttcacggag aagctgctgc
      241 tcgaggcaac gctcgagctg atcacctccg atctcgtcgt tgcggtgcag gacatgggag
      301 cggcggggct taccagctcg tcggccgaga tggcggcgcg gggcggagtc ggcgtcgaaa
      361 tcgacacggg tctggtgccg acgcgggaag acgggatgac gccgtacgaa atcctgttgt
      421 ccgaatcgca agagcggatg ctggtggttg ccgagccgca acggctgcgg gagatccagg
      481 ccgtctgcga gaaatgggag cttacggcga cgccgatcgg ccgggtgacc gacgacggga
      541 tcttcagggt gcggcaccat aaccgtgtgg tagcggcgat tccgggacag ccgctggtgg
      601 acgattgtcc catttaccat cccgaggctc acgaggcaga cgaagcaata tcccgccgcg
      661 cagcgcgccc gtcgtcaacg gcgcagcccg atcctgagaa ggcactcgaa gccctcctgg
      721 atacccccaa cctcgccagc aagcggtggg tctacgagca gtacgactcg accgtgcagt
      781 ccgctacggt gatcggcccg ggcggagatg ccggcgtctt acaagtgccg gggaccgagt
      841 tcggccttgc gatcaccgtt gactgcaatg cccgccgggt cgcgctcgat ccgtacgaag
      901 gtggtaaggc ggtcgtcgcc gaggccgcgc gaaacatcgc ctgcaccggc gccttgccca
      961 tcgggattac cgactgcctg aatttcggga acccggagaa gcccgaggtg ttctttcagt
     1021 tccgcgaggc gtgtcgcggg atctccgatg cctgccgggc cttcgagaca ccggtgaccg
     1081 gtgggaacgt gtcgttctac aacgagagcc cgaccggggc ggtggacccc acgcccgtgg
     1141 ttggcatggt gggcttgctc gagcgagtgg ctgggcgagt gggaagccat tgggtcgaac
     1201 ctggcgacgc gatcctcatt ctcggcgcca ctcagggcga gttgggaggc tccgcctact
     1261 gggccgaagt gctgggcttc gtcgggggcc agccggctcg ggtggacctc gccgccgaac
     1321 gcaggcttca gctcctcctc gtcgcagcag cccgcgtcgg cttgctccga tcggcccacg
     1381 attgcaccga aggcggcttg gcagtcgcgc tggcggaggc ggcgatcggc ggaccgtacg
     1441 ccaagcggag cctgggagcc aggctcgacc tcgaccggta tgccgaagga ctcccggcag
     1501 agcgagtcct ctttggggag gatgacgcgc gcgccgtggt ctcctgtgct ccaggcgcgg
     1561 tgaaagcact gactgcgctt gccggcgagc acggcgtgcc ggcatttgca gctgggacgg
     1621 taagtgcacc ggaaggctgg ctcgagcttc ggatcaccgg caggatgttg acctgggaca
     1681 cgatcgcgct ccggcgcatc tattacgatg cgattccccg gcgcatggcg cacgcggagc
     1741 aggatcgcta ggagacgcta ttcaatgtgc ggcatcatcg gagtctcggg cctcaccgac
     1801 gcggctaaac tcgcctacct gggcctgtac gcgctgcagc atcgcggaca ggaaagcgcg
     1861 ggcatcgtcg ccatcgatcc cgagggccgg ccccggtcgt accggggtat gggcctggtg
     1921 tccgagacct tcgacgataa catcctggcc actctccccg gcgatatcgc ggtggggcac
     1981 acgcggtatt ccaccacggg cagcacggtg ctcgccaatg cacaacc
//
LOCUS       DASRJQ010000330         3704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2551998, whole genome shotgun sequence.
ACCESSION   DASRJQ010000330 DASRJQ010000000
VERSION     DASRJQ010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2551998"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="VFB89_07345"
     CDS             <1..996
                     /locus_tag="VFB89_07345"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZM27154.1"
                     /translation="AYLVAHLAFPRAFAALQLGRLREAEEYGRWSLEQKLARGTTDGR
                     PWHAVPLIDALVEQGDLQAAEEALASLNTPGSPPEQIGWATLLEARGRLRLAQARPEE
                     ALADQLEAGGRFERLRWSHPGIMVWRWDAARALAQLGDQAEAARLAAEQLDLARATEL
                     PRLIGIADRELATVSPSRNALPLLREAIELLERTPARLELAKTAVEYGAALRRSGKRM
                     EARDHLRRGLELAHRAGARPLATRAREELAAAGGRPRKPVFTGIDALTASELRVARMA
                     ATGATNRDIAQRLFVTQKTIESHMRHVFEKLNISRREELPHELATPGNATEPGHD"
     gene            complement(1328..>3704)
                     /locus_tag="VFB89_07350"
     CDS             complement(1328..>3704)
                     /locus_tag="VFB89_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZM27155.1"
                     /translation="PERSAISDLRAGLDLTRRTPLLRLMTWAAVLISALFFLMVFPFA
                     EVVTRSFATEVEMASFLGLFSALATAATFLVSLLGVNRLFARLGVVLTLLILPVVYLV
                     GFATWLASFGLATAVLGRGSQWIAVNAIGSTAWSALFNVVPGRRRGEVMAFMFAVPTQ
                     VGTTAAGALLIAGSELSAAQISAIGLALAGGLLLLVLRMRSAYSAALVDAVRDGNLEV
                     FTLPQAGPQKPLLDADTQRALTGELNAPEAGARAVAVSLLGRLGDGVQADLIVGALHD
                     TDWRVRLAALQALFDQGHPEATGAAITMLGDSAPQVRSLAARSISRLGTNHAESLAAI
                     LEDPDPGVRAVAATGVGGIRAQKTLERMLESDSPADQEAALLALASRPDLTGRDLIRF
                     AGHPDRRVRAAAAAALARLPGSGPVVRSLLDDGSVIVRRASAAGLARRADGPAILMDV
                     LANGSVRATEAALQSLADTGQTGEGLTEWASQEVARAAHLRQHRLALATNDLSPTRTF
                     LASLLASRQERLSRWALLATTTPDTADTMTVVGRGLRSADQETRAQAVEALESVGVHA
                     VTRSLIPLLEDTDHTALPDSRTSLREMSEDHDEWIRALAVRCISEELIDDLRHLRGLA
                     DADQSGLVQSAIARWEVIGVQDTETLDTVDRVVALQRVPMFAGIDPEDLERIATLFTE
                     RRYDTGELIFAWGAEGDEMLIIVEGEVTVSRAPEGSGGAIRTFRSGDYVGELSLLRGQ
                     PRAANVTAGPQGVRGLILRGAELHAILEERPEAAMAMLAALAERLGTDWDR"
BASE COUNT          641 a         1267 c         1258 g          538 t
ORIGIN      
        1 gcctacctgg tcgcgcacct cgcgttcccg cgcgcgttcg cggctctcca actgggtcgc
       61 ctgcgggagg cggaggagta cggtcgctgg tcgctggagc agaagctcgc ccgcgggacg
      121 accgatgggc ggccctggca cgccgtaccg ctgatcgatg cgctggtcga gcaaggtgac
      181 cttcaggcgg ccgaggaagc cctggctagc ctgaacaccc cggggtcgcc tcccgagcag
      241 atcggctggg ccaccctcct cgaggcacga ggccgcctcc gcctcgccca ggcgcgccct
      301 gaggaggcgc ttgccgacca gctcgaggca ggtggacgct tcgagcgcct ccgatggagc
      361 catcccggga tcatggtctg gcgatgggac gccgctcggg cgctggcgca gctcggagac
      421 caggccgagg cagcgcggct cgccgcggag cagctcgacc tggcgcgggc gacggagctc
      481 ccccgcctga tcggaatcgc ggaccgggaa ctggcaaccg tgtcgccgtc tcgtaacgcg
      541 ttgccgctgc tgcgggaggc gatcgagttg ctagagagga cgcccgcgag gctcgagctc
      601 gcgaagaccg ccgttgaata cggagcggcc ctgcgccgtt cggggaagcg gatggaggcg
      661 agggaccacc tccgccgagg cctcgaactc gcgcatcgtg cgggcgcccg accgcttgca
      721 acgcgtgcca gggaggagct cgcggcggca ggtgggcgtc cacgcaagcc cgtcttcaca
      781 gggatcgatg ccctgacggc gagcgagctc cgggtcgccc ggatggcagc gaccggggcg
      841 acgaaccgag acatcgctca gcggctgttc gtaacccaga agaccatcga gtcacatatg
      901 cgccatgtct tcgagaagtt gaacatcagc agacgagagg aactgccgca tgagctcgca
      961 acgcccggaa atgctaccga gccggggcat gactaggaac agcactgcag gcggggagtt
     1021 cgcgacggga tactgctgaa tctggctcta attgatcaag ggttggcgca agcgccggca
     1081 ggcctggcgc gtgtcctcgc tccgctgcgg gccgcgccgc ccccgcccac ctctcaagcc
     1141 tcagagtcgc ctctcagccg ccgtggcagg taaccggccc tccgacacca ctacctccgg
     1201 ccagacgtac ccctcgaacc cccctagagc acaatccagc agacctagtg cactgacgcg
     1261 cgaaggcggc cgggtaaacg tcaatacctc ggtcgccacc ggcgtcaacg cccgcgcccg
     1321 tacgggatca ccggtcccaa tcggtgccga gccgttctgc cagggccgcg agcatggcca
     1381 tggccgcttc tggccgctcc tccagtatcg cgtggagctc ggctcccctc agaatcagcc
     1441 cacggacacc ctgggggccg gctgtgacat tggcggcgcg cggttggccg cgtagcagcg
     1501 agagctcccc gacgtaatcg cccgatcgga aggttcggat agctcctccg gagccctctg
     1561 gcgcgcggct caccgtcacc tcgccctcaa caatgatcaa catctcgtcg ccctctgcgc
     1621 cccaagcgaa gatgagctcg ccggtgtcat agcgacgttc ggtgaagaga gtggcgatcc
     1681 gctcgagatc ttcggggtcg atcccggcga acatcggcac ccgctgaagt gctactaccc
     1741 ggtccaccgt gtcgagtgtc tcggtgtcct gcaccccgat gacctcccat cgtgcgatcg
     1801 ccgattggac caggcccgac tgatcggcat ccgccagccc tcggaggtgc cggaggtcgt
     1861 cgatgagctc ctcgctgatg cagcgtaccg ccagggcacg gatccattca tcgtgatcct
     1921 ccgacatctc gcggagcgac gtccgagaat ccggcagagc ggtgtggtcc gtgtcctcga
     1981 gaagtggaat gagggaacgg gtgaccgcat gcactcccac agattccagc gcctccaccg
     2041 cttgtgccct ggtctcttga tccgccgagc gcaaccccct tccgaccacc gtcatcgtgt
     2101 ccgcggtatc cggtgtggtc gtagccagca gcgcccaacg cgacaagcgc tcctggcgcg
     2161 aggccaggag actcgcgagg aacgtgcgcg tgggagagag gtcgttggtg gccaacgcga
     2221 gacgatgctg gcggaggtgg gcggcgcggg cgacttcctg ggacgcccat tccgtcaggc
     2281 cttcaccagt ttgcccggtg tcggccagcg actgcagggc ggcctctgtg gcgcgaaccg
     2341 acccgtttgc cagcacgtcc atgaggatgg ccggcccatc ggcgcgccgg gcgagaccgg
     2401 cggccgatgc ccggcgcacg atgaccgagc cgtcatcgag caggctacgt accaccgggc
     2461 ccgagccggg tagccgcgcc aacgccgcgg cagcagcagc gcggactcgc cggtccgggt
     2521 gcccggcgaa tcggatcaga tcccgcccag tgagatcggg gcgactcgcc aacgctagga
     2581 gcgctgcttc ttgatccgct gggctgtcgg actcgagcat ccgttccagc gtcttctggg
     2641 ccctgatccc acccacaccg gtcgctgcga cagctctgac accgggatcg ggatcctcca
     2701 ggatggcggc gagagattcg gcatggttgg tgccaagccg tgagatggac cgggctgcca
     2761 gagaacggac ctgtggcgcc gagtctccaa gcatggtgat cgcggcgccg gtcgcctccg
     2821 ggtgtccctg gtcgaacagg gcctggaggg cggcgagcct gaccctccag tcggtgtcgt
     2881 gcaacgcccc gacgatgaga tctgcctgga ctccgtcccc caggcgaccg agcagcgaga
     2941 cggctaccgc ccgcgccccg gcctccggag cgttcaattc accggtgagt gcgcgctggg
     3001 tgtcggcgtc gagcagcggc ttctgcggcc cagcttgcgg gagggtgaag acctcgagat
     3061 tgccgtctcg aaccgcgtcg accagcgccg cggagtaggc ggagcgcatc ctcagcacga
     3121 gtagcaggag cccgccggcc agcgccaagc cgatggcgct gatctgggcg gccgacagct
     3181 ccgacccggc gatgagcagc gcgcctgctg ccgtcgtccc cacctgggtg ggtaccgcaa
     3241 acatgaaagc catgacctcg ccgcgtcgcc gaccggggac gacgttgaac agagcgctcc
     3301 aagcggtcga gccaatggcg ttgaccgcga tccactgtga gccccgcccc aggacggcgg
     3361 tcgccagccc gaacgatgcc agccaggtgg cgaagccgac caaatagacc acaggcagga
     3421 tcaggagtgt cagcaccact ccgagcctgg cgaacaggcg gttgactccc agaagcgaca
     3481 caagaaacgt ggctgcggtc gccaatgcag agaagagtcc caggaagctc gccatctcca
     3541 cctcggtggc gaaggaccgg gtcacgacct cggcgaatgg gaacaccatc aggaagaaga
     3601 gggccgatat caacacggcg gcccaggtca tgagacggag gagtggcgtt cggcgagtca
     3661 ggtcgaggcc ggctcggagg tcggatatgg ccgaccgttc tggc
//
LOCUS       DASRJQ010000331         2339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1693188, whole genome shotgun sequence.
ACCESSION   DASRJQ010000331 DASRJQ010000000
VERSION     DASRJQ010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1693188"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VFB89_07355"
     CDS             complement(<1..389)
                     /locus_tag="VFB89_07355"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HZM27156.1"
                     /translation="MQRLRRRLATGLTLATIAAVASATASAHHSFAMYDQSIKYVFTG
                     VVERVNPDSSHLQIAFVPLNEKRDALVRDSSGKPATWLVEMGGSAASAREGITVNDFP
                     RGTVFSVGLVPLRNGQRGGARVEGLFK"
     gene            complement(556..1446)
                     /locus_tag="VFB89_07360"
     CDS             complement(556..1446)
                     /locus_tag="VFB89_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27157.1"
                     /translation="MRAQRVMEAQLLASVAMLLGLIVTSAAALGAAGQQSAAPGQQSA
                     APAQTPRLADGKPDLTGVWQAVAPRGLAYGFRRCSPYQVECLDWTNQTEDFEFEAPSR
                     LGPNRPLYKPEHWDKIQELDMWTNREDPVMTCQPLGIPRQGPPARIYHTTQDITLLYR
                     GGIDGGGGYAEHRVIPTTGGKHDPKRALEYTYNGYTIGHWEGDTLVLDSIGFTDATWL
                     ARGGFFHSDQLRVVEKFTRTGNDVLYEVTIEDPEVLVEPWVMTPRTLRPVDRPGAGIL
                     PERANCSEVEKGEAASQIRH"
     gene            complement(1484..>2339)
                     /locus_tag="VFB89_07365"
     CDS             complement(1484..>2339)
                     /locus_tag="VFB89_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27158.1"
                     /translation="RGGAPYTPAAGAKDLKAVLFQWTWHMGMLRGVTEPEIVATLEYQ
                     GKGTIQVNGQPCTLTKYRVSTNYQTPGQRTQIECTRSNGEKYSTIETVSGEYAWNEDI
                     PGAEIMPGKGKATPMPATLQERLIRLWASPQGAPKAAIAAAAGLPLSETFAQNPAVLL
                     DKPRPAGAGVATTLSWETGQPVLTFPLPGIPGAVATATLDKGFLAERVVVKHGSATTE
                     FVYGKYDDWNNELNKIEALYAGTIVERRNGAVVRDLTTVQTETGQVYVVMPVPNSVRK
                     AGAGTAAK"
BASE COUNT          352 a          781 c          736 g          470 t
ORIGIN      
        1 catttgaaga ggccttcgac tcgcgccccg cctcgctgcc cgtttcgcag cggcacgagg
       61 ccgacactga agaccgtccc gcgtggaaag tcattgaccg tgatcccttc ccgggcgctc
      121 gccgcggagc cgcccatttc gacgagccag gtcgccggct tgccactgga gtcgcgcacc
      181 agcgcatcgc gcttttcgtt gagcggtacg aacgcgatct ggaggtggct ggagtcaggg
      241 ttgacccgtt ccacgacgcc cgtgaagacg tacttgatcg attggtcgta catggcgaag
      301 gagtggtgcg ccgaggccgt tgccgaggcc accgccgcga tggtggctag cgtaagccct
      361 gttgccaatc tccttctcag tctctgcatc gtgcgcgatc ccctcaggac agccgtgatg
      421 tgttcgcggc cggataccaa tcttaggctg tggacagggg tcgatgcagt tacccgttat
      481 ggggtaccgg gcgcgaaaag cgcccgctca gcgaagctcg ctcgcacgcg agcgaaggtg
      541 tggcgaggtc tgtatctagt gccgaatctg cgacgcggct tctccttttt cgacctctga
      601 gcaattcgct cgctccggca ggatcccggc gcctgggcgg tcgacgggac gcagagtcct
      661 cggcgtcatg acccacggct cgacgagcac ctccggatct tcgatggtca cttcgtagag
      721 cacgtcgttc ccggtccggg tgaacttctc cacgacccgc aactggtcgg aatgaaagaa
      781 gccgccccgc gccagccacg tcgcatcggt gaacccgatc gagtcgagca ccagcgtgtc
      841 accctcccag tggccgatcg tgtagccgtt gtaggtgtac tcgagcgctc gcttgggatc
      901 gtgcttgccg ccggtggtcg ggatcacgcg atgctccgca tacccaccac cgccgtcgat
      961 tccgcctctg tagagcagcg tgatgtcctg ggtcgtgtgg tagatgcgcg cgggcggtcc
     1021 ttgccttggg atgccgagag gctggcacgt catgaccggg tcctcgcggt tcgtccacat
     1081 gtcgagctcc tggatcttgt cccagtgctc cggcttgtag agcggacgat tcggccccag
     1141 ccgtgacggc gcctcgaact cgaagtcctc ggtttgattg gtccagtcga ggcactcgac
     1201 ctgatacgga ctgcaccggc ggaagccgta ggcaagacct ctgggagcca ccgcctgcca
     1261 gacgccggtc aagtccggtt tcccgtcagc cagcctcggg gtctgagccg gcgcggcgct
     1321 ctgctggccg ggcgctgcgc tttgttgccc cgccgcgcct agcgccgccg cggatgtaac
     1381 gatgagaccg aggagcatcg ccacggacgc cagaagctgc gcttccatga ctcgctgtgc
     1441 cctcataacc gaatcctcct cgataccgat gcagtcgcca cgtttacttc gcagccgtac
     1501 ccgcgcccgc ctttcgcaca ctgttcggaa caggcatgac gacgtagacc tggcccgttt
     1561 ccgtttggac cgtcgtcagg tcgcggacca ccgcgccatt ccggcgctcg acgatcgtgc
     1621 ccgcgtacag cgcttcgatc ttgttcagct cattgttcca gtcgtcgtac ttgccgtaga
     1681 cgaactccgt ggtggccgac ccgtgtttca cgaccacgcg ttcggcaagg aagcccttgt
     1741 cgagtgtggc tgttgcgact gctcccggaa ttcccggaag cggaaacgtg agcacgggct
     1801 gcccggtctc ccatgacagg gtcgtcgcca ctcccgctcc tgccgggcgg ggcttgtcga
     1861 gcagcacagc ggggttctgc gcgaacgtct cggacagcgg caggcccgcc gccgccgcga
     1921 tggccgcctt gggcgcgcct tgtggactgg cccacagacg gatcaaccgc tcctggagcg
     1981 tggccggcat gggtgtggct tttcctttgc ccggcatgat ctccgcgccc ggaatgtctt
     2041 cgttccaggc atactcgccg ctcaccgttt cgatggtcga gtacttctcg ccgttggagc
     2101 gtgtgcactc gatctgcgta cgctgtcccg gcgtctggta gttggtgctg acgcgatact
     2161 tcgtcagcgt gcagggctga ccgttcacct ggatggtgcc cttcccctgg tattcgagcg
     2221 tggccacgat ctccggttcg gtcacgcccc gcagcatccc catgtgccac gtccattgga
     2281 agagaacggc cttcaagtcc ttggcaccag ccgccggtgt gtagggcgcg cccccccgg
//
LOCUS       DASRJQ010000332         4164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_571398, whole genome shotgun sequence.
ACCESSION   DASRJQ010000332 DASRJQ010000000
VERSION     DASRJQ010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571398"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VFB89_07370"
     CDS             complement(<1..618)
                     /locus_tag="VFB89_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HZM27159.1"
                     /translation="MTSPLYRDAVPLASIRRTKIVATLGPAWETRERMLALLDAGVNV
                     VRVNASHGTPEIRSRWIRDLKEVIESRSATTSAAILLDLHGPRIRVGQLAEPLVLEAG
                     QNVIFAPEADASGSEIPTAYAGLAKDVQAGSTILLNDGLLNVEVTGVRGDRVLGRVRY
                     GGELRANKGMNLPGVQVSAPAVTQKDREEVERAIALGVDYIGVSFV"
     gene            complement(638..2098)
                     /locus_tag="VFB89_07375"
     CDS             complement(638..2098)
                     /locus_tag="VFB89_07375"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZM27160.1"
                     /translation="MGNAATLERNLSPETPRYDLPGTWITPGFVDGHTHFATWALGRR
                     RVQLAGVRTRAEALDRVRAAGVEAGWVLGQGWDANRWEAPPDRWALDAVRKEPVYLDS
                     LDVHGAWLNTAALKAAGIDRSSVDPFGGRIVRDANGEPTGLLLERAVELALPALPVPP
                     DNLLDAALREAQAEAHRLGVTGIHDVETEAVLAAFRRLENQGALRLRVLFHPPVAALN
                     RLIASGMQSGQGSDWLRIGGVKLFLDGSLGKQTAWMLEPYEGSRECGMPISAFEQARE
                     AIQTASQVGIAATVHAIGDAAVRRALDLLEYAPRVAIPHRIEHLQCVHPDDLGRAARL
                     GVVASMQPAHLLTDIPLIDRYWGSRGAGAYAFRSLLDQGTAVAFGSDVPVAPLDPREG
                     IYAALARRADDDSPSGGWRSEEALGFETALRLYTEGCARAAGNAGHRGRLAPGMDADL
                     VAWELDGASLAAGEAAAFRQARARLTVVDGEVLMQA"
     gene            complement(2614..>4164)
                     /locus_tag="VFB89_07380"
     CDS             complement(2614..>4164)
                     /locus_tag="VFB89_07380"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HZM27161.1"
                     /translation="FAHWEQQLQDFRRTGASIDTTTLDHASRVNLGMLEAEIGKALEV
                     RRCRRELWTVDQLDGPQVGLATLAELQAIRTAAERSLLLERWGRIGRYLDQHVANLRA
                     GLDRGYAAARINVERVIGQLDRIVAQPPDSSPFFAPTGRLKADDPTFKRRLRGLVQDS
                     VLPGFRRYRDFLAATYLARSRPEDGIRWLPNGADCYQALIRNHTSLDLSAAQIHQTGL
                     DEVSSIRAEMLVIVKRRFGTQNLDSVLRAMPEDSTLTFATRKDVFAAAEAATERMERK
                     LPELIGRLPRRRVMVEEMPAYQEKDAPAAYYYPGTADGSRPGRYLVNTYEPRQRPRYT
                     TEVLAYHEGVPGHHIQIALSQELPLPAFRRYGAGTTALVEGWALYTERLADESGMYTD
                     DLSRLGMLFFQAWRACRLVIDTGLHSMGWNRKQAIEYLLTNTGLSRLDAANEVDRYIV
                     DPGQALAYKIGQREITRLRTEAKQALGSKFDLRRFHDVVLDDGAVSLPILADNVHRWI
                     ASQQTHTQ"
BASE COUNT          763 a         1365 c         1310 g          726 t
ORIGIN      
        1 cacgaaagag acgccgatgt agtccacccc gagagctatc gcccgctcca cttcttcccg
       61 gtccttttgg gtgacggcgg gtgcgctcac ctggaccccg ggaaggttca ttcccttgtt
      121 ggcccgcagc tccccgccat accgcactcg acccaacaca cgatcgcccc gcactccggt
      181 gacttccaca ttgagcagcc catcgttgag caggatggtg ctgccggcct ggacgtcctt
      241 ggccagcccg gcgtaggccg tggggatctc ggatcctgag gcgtcggctt ccggcgcgaa
      301 gatcacgttc tgccccgcct cgagaacgag tggttctgcc agttggccga cgcggatgcg
      361 tggaccatgc agatcgagca gaatcgcagc gctagttgtg gcactgcgag actcgatgac
      421 ctccttgaga tctcgaatcc agcgggagcg aatctcgggc gtgccatgag atgcgttgac
      481 ccgcacgaca ttaaccccgg catcgagcag cgcgagcatt cgctcgcggg tttcccaggc
      541 agggccaagg gtagcgacga tcttggtacg gcgaatcgat gcgagcggca cggcgtccct
      601 gtagagcggc gaagtcacgg agtctaatcg tggcgggtca cgcctgcatt aggacttctc
      661 cgtcgaccac ggtcagccgc gcacgggcct gacggaaggc agcggcctcg cccgccgcga
      721 gcgaagcacc gtccagctcc cacgcaacga ggtcggcgtc catgccgggg gcaaggcgac
      781 cgcggtgtcc ggcattgccg gccgcacgcg cacatccctc cgtgtagagt cggagagccg
      841 tctcgaagcc aagagcctct tccgagcgcc acccccccga cggagaatcg tcatcggctc
      901 gccgcgccag cgccgcgtaa atgccttccc gagggtccag cggcgctacc ggcacatcgc
      961 tgccaaaggc taccgccgtt ccctggtcca gaaggctgcg aaacgcatag gctccggcac
     1021 ctcgagagcc ccagtatcgg tcgatcagag ggatgtcggt gagcagatgc gccggctgca
     1081 tcgatgcgac aacgccgagg cgggcggcac ggccaaggtc gtccgggtgc acgcactgga
     1141 gatgttcgat gcgatgagga atcgccaccc ggggagcata ctcgaggaga tcgagggctc
     1201 gccgcacagc ggcatcaccg atcgcgtgga cggtcgcggc gatccctacc tggcttgcgg
     1261 tctggatggc ttcccgcgcc tgctcaaagg cggagatcgg catgccacac tcccggcttc
     1321 cctcgtaagg ctcgagcatc caggccgtct gcttgcccag acttccatcg aggaagagct
     1381 tcacaccacc gattcgaagc cagtcggatc cctggccgct ctgcatccca ctcgcgatca
     1441 ggcggttcag cgccgctacc ggcggatgga agagcacacg caaccgcagc gccccctggt
     1501 tctccagccg tcgaaaggcc gcgagaacgg cttcggtttc gacgtcgtgg atgccggtga
     1561 cgcccaatcg atgcgcctcg gcttgggcct ctcgcaacgc ggcatcgagc agattatctg
     1621 gcgggaccgg aagagccggc aacgcgagct cgaccgctcg ttccagcaag agacctgtcg
     1681 gctcgccgtt cgcatcgcgc acgatccgac cgccgaaagg atcgacgctg gaccggtcga
     1741 tgcccgctgc tttcagggcc gccgtgttga gccatgcgcc gtggacgtcg agcgagtcca
     1801 ggtacaccgg ctccttgcgc acggcatcga gcgcccagcg gtcgggcgga gcttcccatc
     1861 gattcgcgtc ccatccctgc ccgagaaccc aacccgcttc aactcccgcg gctcgcaccc
     1921 ggtccagcgc ctccgccctg gtgcgcacgc cagccagttg aactcgtcgc cggcccagag
     1981 cccaggtggc aaagtgggta tgcccgtcga cgaatcccgg agtaatccag gtcccaggca
     2041 ggtcatagcg cggtgtttca ggggacaggt tgcgttccaa agttgctgcg tttcccaccg
     2101 cgcgcactcg ccccgcctgc caccaaaccg cgtcgccgtc tatcccgggc gcgagcgaaa
     2161 ggacgcggcc gcctcttaga aagccgtccc ggagccgatc cgagcgactt gctcctgcaa
     2221 cgctatccac atgtggaaaa gccgacaaag tgaatatcca aatggggtgt ctaagagttg
     2281 aaaatcgatg cgttcaggct gagatgacgc ttgaagtcag aaggctaact agcgtgaaca
     2341 ccaaagcttg cggcgcggca tcttaagcag atcgaaggat ggcgacagtg tcggcaccgc
     2401 tataggaatt atgcatacga attacttacg cacattgtcg acaccagctt tttcagggtt
     2461 ttggtcacca ctgtgtaacc taaatcacat gtttgcattg gggtctgcgg agcgggttcc
     2521 gctctgtaga acggcggcag agccggcagt atcgcggcac cagcgtcccc ctctttaccg
     2581 acgaagctgg tgcgtcgcgt cccgctgcag gtgtcactgg gtgtgcgtct gctgagaggc
     2641 aatccagcgg tgaacgttgt ccgcgagaat cggcagactg accgcgccgt cgtccaagac
     2701 cacatcgtga aaacgccgaa ggtcgaattt ggagccgagc gcctgcttcg cctccgtgcg
     2761 gagccgcgtg atttcccgct gtccgatctt gtaggcgagc gcctgaccgg ggtcgacgat
     2821 gtagcgatcg acctcattcg cggcatcgag ccggctcagg ccggtgttgg ttaggaggta
     2881 ttcgatcgcc tgcttccgat tccagcccat cgaatgaagc ccggtatcaa tgaccagccg
     2941 gcacgcccgc cacgcctgaa aaaacagcat gcccagccgg ctcaggtcgt cggtatacat
     3001 ccccgattcg tcggccagac gttcggtgta caacgcccag ccctcgacca acgccgtggt
     3061 ccccgctccg tagcgacgga aagccgggag tggcagctcc tggctcaggg cgatctgaat
     3121 gtggtgtccc ggaacgccct cgtgatacgc cagcacctcg gtggtgtatc gcggccgctg
     3181 gcgaggctcg taggtgttga ccaggtaccg tcccggacgg ctgccgtccg cggtgcccgg
     3241 gtagtagtac gccgccggtg cgtccttttc ctggtaggct ggcatctcct cgaccatcac
     3301 ccggcggcgt ggaagccgcc cgatcagctc gggaagcttt cgctccatgc gttcggtcgc
     3361 ggcctcggct gccgcgaaca cgtccttccg tgttgcgaag gtaagggtcg agtcctccgg
     3421 catggcccgg agcaccgaat ccaggttctg ggtgccaaat cgccgcttga cgataaccag
     3481 catctcggca cgaatcgacg atacctcgtc gagacctgtc tgatgaattt gcgctgccga
     3541 aaggtcgaga gaggtgtgat tgcggatcag ggcctggtag cagtcggcgc cgtttggcag
     3601 ccagcgaatc ccatcctcgg gacgcgaccg cgctaggtag gtcgcggcga gaaagtcacg
     3661 gtaacgccgg aagccaggaa gcacggagtc ctgcacaagg cctcgaagcc ttcgcttgaa
     3721 ggtgggatcg tctgccttga gccgccccgt gggagcgaag aatggagatg aatcaggtgg
     3781 ctgggccacg attcgatcca actggccgat gacccgttcc acgttgatcc gggccgcagc
     3841 gtagccccgg tccaggccgg cccgaaggtt ggccacgtgc tgatcgagat accggccgat
     3901 gcgcccccac cgctcgagca acaacgatcg ctcggcagcg gtgcggatgg cctggagttc
     3961 ggccagcgtg gcgaggccga cctgaggacc gtccagctgg tccacggtcc agagctcacg
     4021 gcggcagcgg cgaacctcga gcgcctttcc gatctccgcc tccagcatgc ccagattcac
     4081 tctggaggcg tgatcgaggg tcgtcgtatc gatgctcgca cccgtgcgcc gaaagtcctg
     4141 tagctgttgc tcccaatggg caaa
//
LOCUS       DASRJQ010000333         4191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2612845, whole genome shotgun sequence.
ACCESSION   DASRJQ010000333 DASRJQ010000000
VERSION     DASRJQ010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2612845"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /gene="xseA"
                     /locus_tag="VFB89_07385"
     CDS             complement(<1..718)
                     /gene="xseA"
                     /locus_tag="VFB89_07385"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HZM27162.1"
                     /translation="MTLPLPLSSALAGDEVLSIRDLTASLKRVVDGSYGPLWVRGEVL
                     QCRAPSSGHWYFVLRDAAAQVRCTMWRTYAERAGRPPVDGTEVFALGRPDVYESKGEL
                     QFMVTRMLPTSMVGDQQRALEQVKAALDRDGLFDQARKRPLPAYPARVAVVTSTDGAA
                     LSDIITVARTRWPSVRLFVLASKVQGDGATAELVAALRRVNRLDVQLCIVGRGGGARE
                     DLAAFNDEAVCRALAAVRVPT"
     gene            complement(759..1640)
                     /locus_tag="VFB89_07390"
     CDS             complement(759..1640)
                     /locus_tag="VFB89_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HZM27163.1"
                     /translation="MTARVLDGVPIADAIRAEVAARVAEHRKRGRQPGLAVVIVGDNP
                     ASAVYVRAKGKACQEAGMYSETVRLPAETSEAELLAVVDRLNTDPKIHGFLVQLPLPK
                     HINAERVLNRINPLKDVDGFHPANVGKVSIGDPAGLKPATPYGVQEMLLRSGLDTKGA
                     HAVIVGRSNIVGKPMASLLIQPGRGGDATVTVCHTKSRDLPGICRTADLLIVAMGKPE
                     FVTAEMVRPGAVVIDVGINRVDDASQPKGYRIVGDVAYGPVSEVASAITPVPGGVGRM
                     TIAMLLQNTLQAFEQAS"
     gene            complement(1637..3241)
                     /gene="rny"
                     /locus_tag="VFB89_07395"
     CDS             complement(1637..3241)
                     /gene="rny"
                     /locus_tag="VFB89_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HZM27164.1"
                     /translation="MTNVSDMLAHLDRTVLIVLGAAIGLAIAVVVDLVRGRMQRRYGR
                     GIVEEARSEATRLTTDAAREAEAAKNAGVVAGKMEALKLREELDREIQRRREEWERAE
                     RRVEDRSRSLERKLEEVAGQERQFRVQEQALAQRELDLRAREGEIDKLVQEQRSRLER
                     IAGLTAEDARREILQRVEDEARTQASALTRDIKEQARRTADRDAKRIIAMATQRLAAE
                     HTAETTVAAVALPSDEMKGRIIGREGRNIRAFETATGVDVIIDDTPDTVIISCFDPIR
                     REVARRALEALIADGRIHPGRIEEIVAKCQRELETQLVELGEQAAYDTGIHGLHPELI
                     KLVGRMRYRTSYGQNILDHSKEVSWLAGMMASELHLDVALAKRGGLLHDIGKVLTHDT
                     EGTHVELGVEIARKYGESAAVINCIHAHHDDVPHETAESVLVQAADSISGSRPGARRE
                     AFESYVKRLTRLEEIANGFAGVEKSNVIQAGREIRVVVTPERIDDTAAAHLSDSIARR
                     IENELQYPGQIKVVVIRETRAVDFAR"
     gene            complement(3241..3567)
                     /locus_tag="VFB89_07400"
     CDS             complement(3241..3567)
                     /locus_tag="VFB89_07400"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HZM27165.1"
                     /translation="MTGPKNAVRVIIGGEEYTVKSELPPEYTREVAAYLDAALKRVRD
                     SLPGVETHKAAILAALSITDELFQARRGDREIATRVSALADDLARLLPPAKRGHRTAG
                     TVPVPE"
     gene            complement(3564..3869)
                     /locus_tag="VFB89_07405"
     CDS             complement(3564..3869)
                     /locus_tag="VFB89_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27166.1"
                     /translation="MTYNYERPDLKAVGELEELFQHLGAEVAAWRRRSLKAEGELQEI
                     RAKGGVYEGPELSGARQRALELETENQALRERIEAAKERVRSLAARLAFLEQGGTAS"
     gene            complement(3866..>4191)
                     /locus_tag="VFB89_07410"
     CDS             complement(3866..>4191)
                     /locus_tag="VFB89_07410"
                     /inference="COORDINATES: protein motif:HMM:NF015129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27167.1"
                     /translation="LPSTPAAERDLALVIPDGIVASRVRQTLEEAAGPLLVGLDVLDE
                     YRGAGLPSGVRSAMFRLTFRAPDRTLRDQEIDEVERHVLAVLERELGARRREAAPSSS
                     PGRVP"
BASE COUNT          679 a         1460 c         1307 g          745 t
ORIGIN      
        1 ccgtggggac ccgcacggca gccaaagccc ggcaaaccgc ctcgtcgttg aacgcggcca
       61 gatcctcgcg cgcaccaccg ccccgaccga cgatgcagag ctgcacgtcg aggcggttga
      121 cccgccggag tgctgccacc agctctgccg ttgcgccgtc tccctgcact ttcgaggcga
      181 ggacgaacag ccgcacggac ggccaacgcg tgcgagcgac cgtaatgatg tcgctgagtg
      241 ccgcgccgtc ggtgctcgtg acgacggcga cgcgagccgg ataagccggc aacggacgct
      301 tccgcgcctg atcgaaaaga ccgtcccgat cgagcgccgc cttgacctgt tcgagggcgc
      361 gctgctggtc gcccaccatc gaggtgggaa gcatacgcgt gaccatgaac tggagctcgc
      421 ccttcgactc atacacatcg gggcggccga gcgcgaaaac ctcggttccg tcgaccggag
      481 gtcgtcccgc gcgctcggca taggtgcgcc acatggtgca gcgcacctgt gcggcagcgt
      541 cgcgcaacac gaaataccag tggccgctcg agggcgcccg gcactggagg acttcgccgc
      601 ggacccaaag cggcccgtag ctgccatcga ctacgcgctt gagcgaagca gtgagatcgc
      661 ggatcgagag cacttcatca ccggccagcg ccgatgagag cggaaggggc agggtcatcg
      721 aaagaagtgc ccgcggctgc gaacgggccg acaccaagtc atgaagcctg ctcgaaggcc
      781 tgaagagtgt tctgcagcag catcgcgatc gtcatccgtc ctacgccacc cggcacgggc
      841 gtgatggcgg aggcgacttc cgaaacgggt ccgtacgcga cgtcgcccac gatgcgatat
      901 cccttgggct ggctggcatc gtccacccgg ttgatcccga cgtcgatcac caccgcgccg
      961 ggccgcacca tctcggcggt aacgaactcg ggcttcccca tcgccacgat gagaaggtcg
     1021 gcggtgcggc agatgccggg aaggtcccgc gacttcgtgt ggcagaccgt caccgtcgcg
     1081 tcgcccccgc gacccggctg gatcaacaga ctcgccatcg gcttcccaac gatgttcgag
     1141 cggcccacga tcactgcgtg agcgcctttg gtgtcgagcc ccgagcggag cagcatctcc
     1201 tgcacgccgt acggtgtagc cggcttgaga ccggccggat cgccaatcga caccttcccc
     1261 acgttggcgg ggtggaagcc gtccacgtcc ttgagagggt tgatccggtt gagtacccgc
     1321 tcggcgttga tgtgctttgg cagagggagc tgcaccagaa agccgtgaat cttgggatcg
     1381 gtgttgagac ggtctaccac agcgagcagc tccgcttccg aggtctctgc cggaagccgg
     1441 accgtctcgg agtacatacc cgcctcttgg cacgccttgc ccttggcgcg tacgtagacg
     1501 gcgctggcag ggttgtcccc cacaatgaca acggccagcc cgggctgccg accccgcttc
     1561 cgatgctcgg ccacccgcgc ggccacttct gcccggatcg cgtcggcgat gggcacaccg
     1621 tcaagaaccc gggccgtcat cgggcgaagt ctacagcgcg ggtctcacgg atcacgacca
     1681 ccttgatctg gccgggatac tgcagctcgt tctcgatacg ccgggcgatc gaatccgaca
     1741 ggtgcgcggc tgccgtgtcg tcgatccgct ccggcgttac cacgacgcgg atctcgcgtc
     1801 ccgcctgaat cacgttgctc ttctccactc cggcgaatcc gttggcgatt tcttcgaggc
     1861 gggtgagacg cttcacgtag ctctcgaagg cctcgcgccg agcgccgggg cgggacccgc
     1921 tgatcgagtc ggccgcctgc accagcaccg attccgcggt ctcgtgggga acgtcgtcgt
     1981 ggtgcgcgtg gatgcagttg atcacggcgg ccgactcccc gtacttgcgc gcgatctcga
     2041 cacccagctc gacgtgggtt ccttcggtat cgtgagtcag caccttgccg atgtcgtgca
     2101 acagaccgcc ccgcttggcc agcgccacgt cgaggtgaag ctcactagcc atcatccccg
     2161 ccagccagga cacttccttc gagtgatcga ggatgttctg cccgtaggag gtgcggtatc
     2221 gcatcctgcc gaccagcttg atgagctcgg gatggaggcc atgaatcccg gtgtcgtagg
     2281 ctgcctgttc ccccagctcg acgagctgag tctccagctc ccgctgacac ttggcgacga
     2341 tttcctcgat gcgtccggga tgaatccgtc cgtcggcgat caacgcctcg agcgcgcgcc
     2401 gcgccacttc gcgccggatg gggtcgaagc aggagatgat gaccgtgtcc ggcgtgtcgt
     2461 cgataatcac atcaacgccg gtggcggtct cgaaagcccg gatgttccga ccttcgcggc
     2521 caatgatacg gcccttcatc tcgtcgctcg gcagcgccac ggcagcgaca gtggtctcag
     2581 cggtatgctc cgccgccagc cgctgcgtcg ccatggcgat gatccgcttg gcgtcgcggt
     2641 ccgcggtccg gcgggcctgc tccttgatgt ctcgcgtcag ggcggaggcc tgggttcgcg
     2701 cctcatcttc cactcgctgg aggatctcgc gccgagcgtc ctctgcggtc agccccgcga
     2761 ttctctccag ccggctccgt tgctcctgca cgagcttgtc gatttcgccc tcccgggcac
     2821 gcaaatccag ctcacgctgc gccagcgctt gctcctgcac ccgaaattgc cgttcctgcc
     2881 ccgcgacctc ctccagcttg cgctcaagag agcggctccg atcctcgacc cgtcgctcgg
     2941 ctcgctccca ttcctcgcgc cttcgctgaa tttcccggtc gagctcctca cgcagtttga
     3001 gcgcctccat cttgcccgcc acgaccccgg cattcttggc agcttccgcc tcgcgcgcag
     3061 cgtcggtagt gagccgggtt gcttcggagc gggcttcttc cacgatgcca cggccatacc
     3121 gccgctgcat tcggccgcgg accaggtcca cgaccactgc aatcgcgagg ccgatcgcgg
     3181 cgccgagcac gatgaggacg gttcgatcga ggtgggccag catgtcgctc acgttcgtca
     3241 ttattcaggg accggtacgg tgccggcggt gcggtggccc cgcttggccg gcggaaggag
     3301 ccgcgcaagg tcatccgcga gtgcagatac tcgggtcgcg atctcccggt cccctcgacg
     3361 ggcttgaaac agctcatcag tgatggataa ggccgcgagg atggcggcct tgtgggtctc
     3421 tacgccgggc agcgaatccc gcacccgctt gagtgctgca tcgagatagg cagcgacctc
     3481 gcgcgtgtat tctggcggca actccgattt gactgtgtac tcctcgccgc cgatgatgac
     3541 ccggaccgca tttttgggcc ctgtcacgat gcggtgcctc cctgttcgag aaacgccagc
     3601 cgtgccgcga gcgagcgcac ccgctctttg gctgcctcga tccgctctcg caacgcctgg
     3661 ttctcggtct cgagctccaa ggcccgttgg cgcgccccgg acagctcagg accctcgtag
     3721 acaccgccct tggcgcgaat ctcctgcagt tcgccttcag ccttgaggga tcgacgccgc
     3781 caggctgcaa cctcggcacc tagatgctgg aaaagctcct ccaactcacc aacggctttc
     3841 agatccggtc gctcgtagtt gtaggtcatg gcactctccc tgggctgctc gacggcgcag
     3901 cctcacgccg gcgtgcaccc agctcccgtt cgaggaccgc caagacgtgt cgctcaacct
     3961 catcgatctc ctggtcgcgc agggtccggt cgggagcgcg gaaggtgagc cgaaacatgg
     4021 cgctgcgtac tccggaaggc aggcctgcac cccgatactc gtccagcacg tccaagccaa
     4081 ccaatagagg tcccgcggcc tcttccagcg tttgacggac cctgctggcc acgatcccat
     4141 cagggattac cagagcaagg tcccgctcgg ctgccggcgt agatggcaac g
//
LOCUS       DASRJQ010000334         2413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3186806, whole genome shotgun sequence.
ACCESSION   DASRJQ010000334 DASRJQ010000000
VERSION     DASRJQ010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3186806"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VFB89_07415"
     CDS             complement(<1..266)
                     /locus_tag="VFB89_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27168.1"
                     /translation="MKTLVSTRASLFLYVASCAAAILLGAATPQPQPAQIGRPEVRTA
                     KPPSTVFVPAPQRIVIDRNALAAATEGVWLQKLTTGFLRRCTGRS"
     gene            499..1083
                     /locus_tag="VFB89_07420"
     CDS             499..1083
                     /locus_tag="VFB89_07420"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HZM27169.1"
                     /translation="MGKSRFEAFSDGVIAIIITIMVLEMKVPHGESLQALRPLIPVFL
                     SYTLSFVYLGIYWNNHHHMLHTVHKVTGPMLWANLHLLFWLSLVPFATGWMGENHFSS
                     MPSALYGVVLLMAAIAYYILQQLIIASQGPGSLLKKAVGGDWKGRASPFFYAIAIPMA
                     FWSQWISLGLYVLVALIWLVPDRRIEHLLASQEA"
     gene            1088..1375
                     /locus_tag="VFB89_07425"
     CDS             1088..1375
                     /locus_tag="VFB89_07425"
                     /inference="COORDINATES: protein motif:HMM:NF021552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HZM27170.1"
                     /translation="MCRNIKTLHNFEPPATDGEVRASALQFVRKLSGFTKPSKANEEA
                     FNRAVDQVTQAARELLGSLVTNAPPRDREVEATKARAKAAVRYGSPINAGG"
     gene            complement(1406..>2413)
                     /locus_tag="VFB89_07430"
     CDS             complement(1406..>2413)
                     /locus_tag="VFB89_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27171.1"
                     /translation="EEAAPTQQPNWLADRDGNLVWYEILIDKPEYDYIAQNGFYNAET
                     QLQKVSSGVHINLPRGVYNAGQGAMELKAAWLTVQDPKAEKWRRYKLSRALAYDPRHD
                     TCDFITVALVGLHILHKTETQPQWTWATFEHVDNAPDPSQVATAGERYTFYRSDCKPQ
                     PVSAHCQNVVKKGCQPPDDNDQTSCTPNTEPAYCLQLDGEPDKLCRPYPIQVVREMPI
                     PDSNDNPIQRLNKGVQEMYRKANPDSVWQYYQLVDVLWSDSAVDENLAPKPPVTPLSQ
                     SGLRPSPPSLPVSNAVLETYVQNLACVNCHHSATIADAQDPAFASDYTFVLGMAKSPK
                     K"
BASE COUNT          401 a          723 c          826 g          463 t
ORIGIN      
        1 gatctgccgg tgcaccgccg gaggaaaccg gtggtgagct tctggagcca gacgccctcg
       61 gtcgccgcgg cgagcgcatt cctgtcgatc acgatccgct ggggggccgg gacgaacacg
      121 gtgctcggcg gcttggccgt ccgcacctcg ggccggccga tctgcgccgg ctgcggctgc
      181 ggggtggcag cgccgagcag gatcgcggcc gcacacgagg ccacatagag gaagagggag
      241 gcacgggtgg atacaagagt tttcatcttc actgtcatct cctttgggat ttgtcgacga
      301 acagccatgc aggcgcgctg gccggtgcct gcgttacaaa aaaacagacg caaaaaatga
      361 ttggaaaaaa gtgagggaaa gtgccgggcc ggagcgtctt ttcccagatg gcggtcgggg
      421 gttgcgggag aggccgtctc cgatctagga tcgggcgtgg ggagaacgga gatcaaccct
      481 tggacacgcg aggcaggaat gggaaagagc cggttcgaag cgttcagcga cggcgtgatc
      541 gccatcatca tcacgatcat ggtgctggag atgaaggtgc cgcatggcga gagccttcag
      601 gctctgcggc ctttgatccc ggtgttcctc agctacacgc tgagcttcgt ctacctgggc
      661 atctactgga acaaccatca ccacatgctg cacacggttc acaaggtgac cgggccgatg
      721 ctctgggcca atctgcatct cctgttctgg ctgtcgctgg ttcctttcgc cacgggatgg
      781 atgggagaga accacttttc ctcgatgccg tcggccctct acggggtggt gctgctgatg
      841 gccgcgattg cctactacat cctgcagcaa ctgatcatcg catcgcaggg tcccggctcg
      901 ctgctgaaga aggctgtcgg cggtgattgg aagggcaggg cctcgccgtt cttctacgcc
      961 atagccattc ctatggcctt ctggtcgcag tggatctcgc tgggcctcta tgtcctggtc
     1021 gccctgatct ggctcgtgcc ggatcgacgc atcgagcacc ttcttgcaag ccaggaggca
     1081 tgacgccatg tgccgaaaca tcaagaccct gcacaacttc gagccgccgg ccacggatgg
     1141 cgaggtgcgg gcttccgccc tgcagttcgt gcgcaagctg agcggcttca cgaagccctc
     1201 caaggccaac gaggaggcgt tcaaccgcgc cgtggaccag gtgacgcagg cggcccgcga
     1261 gctactcggc tccctggtca cgaacgctcc gccccgcgac cgcgaggtcg aagctacgaa
     1321 ggcccgcgcc aaggccgcgg tccgctatgg ctccccgatc aacgccggcg ggtgaagccc
     1381 gccggcgttc ggggcgcaag ggcagttact ttttgggtga cttggccatg cccagcacga
     1441 aggtgtagtc cgaggcgaag gcggggtcct gggcgtccgc gatggtggcg gagtggtggc
     1501 aattaacgca cgccaggttc tggacgtagg tctcgagcac cgcgttggac acgggcaacg
     1561 acggcggcga gggccgcaac ccgctctgcg acagcggcgt caccgggggt ttcggagcga
     1621 ggttctcgtc caccgccgaa tccgaccaga ggacgtcgac gagctggtag tactgccaca
     1681 ccgaatcggg attggccttg cggtacatct cctggacgcc cttgttgagc ctctggatcg
     1741 ggttgtcgtt cgagtccgga atgggcatct cgcgcaccac ctggatggga tagggacggc
     1801 acagcttgtc cggttcgccg tcgagctgca ggcagtaggc gggctccgtg ttgggcgtgc
     1861 agctcgtctg gtcgttgtcg tcgggcggct ggcagccctt cttgaccaca ttctggcagt
     1921 gcgccgacac cggctgcggc ttgcaatcgg agcggtagaa ggtgtagcgc tcgcccgccg
     1981 tcgcgacctg gctggggtcc ggcgcgttgt ccacatgctc gaaggtggcc caggtccatt
     2041 gcggctgggt ctcggtcttg tgcaggatgt gcagtccgac cagggccacg gtgatgaaat
     2101 cgcaggtgtc atgccgcggg tcgtaggcga gggcgcgcga cagcttgtag cggcgccact
     2161 tctcggcctt gggatcctgc acggtcagcc aggccgcttt gagctccatc gcgccctgtc
     2221 cggcgttgta gaccccccgc ggcaggttga tatggactcc gctcgacact ttctgaagct
     2281 gggtctcggc gttgtagaag ccgttttgcg cgatgtagtc gtattcgggc ttgtcgatca
     2341 ggatctcgta ccagacgaga ttgccgtcgc ggtcggcgag ccagttcggc tgctgcgtgg
     2401 gggccgcctc ttc
//
LOCUS       DASRJQ010000335         2200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1814882, whole genome shotgun sequence.
ACCESSION   DASRJQ010000335 DASRJQ010000000
VERSION     DASRJQ010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1814882"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1904)
                     /locus_tag="VFB89_07435"
     CDS             complement(<1..1904)
                     /locus_tag="VFB89_07435"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM27172.1"
                     /translation="MIRDVLRDLKYALRSFARRPLFTGVILLTLALGIGSNVAIFSVA
                     NAVLFRALPFRNPGELAFVWTRLPKTNVSRALVSGPDFKDYQTETTRFTGFAGAVALP
                     GNITGEGPPERITNAYVTWNMLEILGVRPILGRTHRAEDAFPMDPKQFGNPDAKLPPN
                     KVVLSYGLWQRRFGGDSAVIGKTILLDGWGSLVIGVLPRDFRIYLPPDAGMPSNIDAW
                     GILPSNIGDFERQAAWLTVVTRLKEGVTLEQGQEDMDRLAARLREVHQFHKTQDLQIV
                     VAGMHRDVVEHVRPALVALLGAVGFVLLIACANIANLLLVRASERNREIAVRAALGSG
                     RGRIVAQMLTESLVLAAAGTVLGLVLAWQGIRVIKALSPANLPRIESVSLDGPAFAFA
                     AAVAVVAAILFGLAPALRAVRGNLADGLRDRGTDTGGARGNKLRTVLVVSEVTLSLVL
                     LIGAGLMVRSFAAIQKVNPGFEAKNVVTFNVPIPFLKYLTSQLRATFINQLGDRLATI
                     PGVESVGGVTPLPLAGGEQYSVGSYGKIGDSDDAYRTNKADFKAVLPGYFETMKIALV
                     SGRTFVRSDNEAQALDVAMVDAKFAARVFGKENPLGAQILVDHFNEQTFSMERLPVTI
                     VGVVANVRSAS"
     gene            complement(1938..>2200)
                     /locus_tag="VFB89_07440"
     CDS             complement(1938..>2200)
                     /locus_tag="VFB89_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27173.1"
                     /translation="AMIMFKALGVISDGKEVSVEPLPNYNREPAFSWDAYLDVPDPDA
                     LAAEFAARGVPFAVPLTEGDGLRGFVIEDLDGYGLYFGCVRA"
BASE COUNT          397 a          753 c          706 g          344 t
ORIGIN      
        1 gatgccgatc ggacgttcgc caccaccccc acgatggtta cgggcagccg ctccatcgag
       61 aaggtctgtt cgttgaagtg atcgaccagg atctgggccc ccaggggatt ctctttgccg
      121 aagacccggg ccgcgaactt cgcgtccacc atcgcgacgt ccagcgcctg agcctcgttg
      181 tccgagcgca cgaacgtgcg gcccgacacc agcgcgatct tcatcgtctc gaagtatccc
      241 ggcagcaccg ccttgaagtc cgccttgttg gtccgatagg catcgtccga gtcgccgatc
      301 ttgccgtacg agccgaccga atactgctcg ccgccggcaa gcggcaacgg ggtgactcct
      361 cccaccgatt cgactcccgg aatcgtggcc agtcggtccc ccagctggtt gatgaacgtc
      421 gcccgcaatt gtgaggtcag gtacttcaga aacgggatcg gcacgttgaa ggtcacgacg
      481 ttctttgcct cgaacccggg gttcactttc tggatcgcag cgaagctgcg caccatgagc
      541 ccggccccga tcagcaggac cagcgacagc gtcacctcgc tgaccacgag cacggtgcgg
      601 agcttgttgc cccgggcccc gccggtgtcg gtgccgcggt ccctgagacc gtccgccaga
      661 ttgccccgga ccgcgcgaag cgcaggtgcc aggccgaaga ggattgccgc caccacggcg
      721 acggcggcgg cgaacgcaaa cgcgggccca tcgagagaca cgctctcgat cctcggcagg
      781 ttggcggggc tgagggcctt gatcacccgg atcccctgcc aggcgagtac cagtccgagc
      841 acggtgccgg cggcggcgag caccaggctc tcggtgagca tctgcgccac gatgcggccc
      901 cgcccgcttc cgagcgccgc gcgcaccgcg atctcgcggt tgcgctcgga cgcccgcacc
      961 agaagcaggt tcgcaatgtt ggcgcaggcg atcagcaaca cgaagcccac cgcgcccaga
     1021 agcgccacca gggcaggccg aacgtgctcg acgacgtcgc gatgcatccc ggccaccacg
     1081 atctggaggt cctgggtctt atggaactgg tggacctccc gcagccgcgc cgccaggcgg
     1141 tccatgtctt cctggccctg ctccagcgtg acaccctcct tgagccgggt caccacggtg
     1201 agccaggcgg cctgccgctc gaagtcgccg atattgctcg gcaggatgcc ccaggcgtcg
     1261 atgttcgacg gcatgccggc gtctgggggc aggtagatgc ggaagtcccg cgggagcacc
     1321 ccgatcacga gcgagcccca gccgtccagg aggatggttt tgccgataac cgcggaatcg
     1381 ccgccgaagc gccgctgcca gaggccgtag ctcaggacca ccttgttggg cgggagcttg
     1441 gcgtccgggt tgccgaactg cttgggatcc atcggaaacg catcttctgc ccggtgggtc
     1501 cggcccagga tcggccggac gcccaggatc tccaacatgt tccaggtgac gtaggcgttg
     1561 gtgatccgct ccggcgggcc ctctccggtg atgttcccgg gaagcgccac cgccccggca
     1621 aaccccgtga atcgcgtggt ctcggtctgg tagtccttga agtcgggtcc cgagaccagc
     1681 gcgcgcgaca cgttcgtctt gggaagccgg gtccacacga acgccagctc ccccgggttc
     1741 ctgaacggca gggcgcggaa gaggacggcg ttggccacgc tgaagattgc cacgttgctg
     1801 ccgatgccga gcgccagcgt gagcaggatg acgccggtga agagcggccg cctggcgaat
     1861 gagcggaggg cgtacttcag gtcacggagc acatcccgaa tcatgacgag actccacagg
     1921 gcagggagac gaccggctta cgcgcgaaca cagccaaaat acagaccata gccatcgaga
     1981 tcctcgatca cgaagccccg gagaccatct ccctccgtga gaggtacggc gaaaggaacc
     2041 ccgcgcgcgg caaactccgc cgccaacgcg tccggatcag gcacgtccag ataagcatcc
     2101 caactgaacg ccggttcacg gttgtagttc gggagcggtt cgacgctgac ctctttgccg
     2161 tcggagatca cgccgagcgc cttgaacatg atcatcgccc
//
LOCUS       DASRJQ010000336         6813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3997499, whole genome shotgun sequence.
ACCESSION   DASRJQ010000336 DASRJQ010000000
VERSION     DASRJQ010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6813
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3997499"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VFB89_07445"
     CDS             complement(<1..596)
                     /locus_tag="VFB89_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZM27174.1"
                     /translation="MPRGGPFTVYGVWGVVMPIYHSLGSMPRKRHTVFRKPDGGIYAE
                     ELMGHEGFVGTSSLLYHVHPPTTVKSARRVKEIVWEADDDTSLRHRHFLTARAKKGGS
                     PTLDRIPILFNHEIAMLYVEPDEQDAHFYRNSMADEVAYVVEGTGVLESVFGDLPFRA
                     GDYVIIHRNITHRWRLDLAAGPTKLLVFESRGHVRWPKR"
     gene            complement(634..1758)
                     /gene="hppD"
                     /locus_tag="VFB89_07450"
     CDS             complement(634..1758)
                     /gene="hppD"
                     /locus_tag="VFB89_07450"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HZM27175.1"
                     /translation="MPTEALGTAPATTDTFPINGTDFIEFYVGNAKQASLYYCAAFGY
                     RLTAYRGPETGVRDRASYALEQDKVRLVLTTALRPDHPIAAHVNAHGDGVKDIALWVD
                     DTRTAFRNAVERGAKPAYEPTVLKDDDGEVVIAGIHTYGETIHSLVERRNYRGAFLPG
                     FRAVDSRFNPPPVGLQYVDHCVGNVELGAMNRWVQYYEQVLGFYNLISFDDKTISTEY
                     SALMSKVVANGNGRIKFPLNEPAQGRKKSQIDEYLEFYRGPGVQHIAIATDDIVATVT
                     ALQNRGVEFLRVPLTYYDTVLDRVGRIDEDIAPLRELGILVDRDDEGYLLQIFTKPVQ
                     DRPTLFFEIIQRKGAKSFGAGNFKALFEAIEREQAARGNL"
     gene            1894..4119
                     /locus_tag="VFB89_07455"
     CDS             1894..4119
                     /locus_tag="VFB89_07455"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HZM27176.1"
                     /translation="MCASPRTFAILTALIRITPFAFLPFSFSPVFGQGWIEIERPVLP
                     GRPLGNVVRTSSDVRVTVNGHVAQIAVEERFRNDGGGLAEGSYLYPLPSEAVFQNFSL
                     WNGETELKGEMLNAEQARRVYEEIVRRRKDPALLTLAGHRLIRAQVFPIQPGETRKVV
                     LRYTQLLTRAGDALRLRYAAGDRGGTSVPGFRVTVENADQVGEPYSPTHRLETRREGN
                     HLQIALASNSAGDVELFFPLAKGLVAASVVTHAPVGEDGYFLLLMAPGPETRETSLPR
                     DVTLVVDVSGSMSGEKLEQAQAGLRQALGTLALQDRFRLIAFSSGVREFRPGFTAASQ
                     AAIDSARRFVDDLSADGGTNIAGALDAALDGTVESNRLPIVLFLTDGLPSIGEQAPDR
                     IAERASQRLAGRRIFMVGVGHDVNTYLLDRLAIEGRGSVEYVAPGSSVETAIGTITTR
                     IRHPALVNLRIESSPVGLSELAPGQLPDLFQGEELVVLGRYHGHRSGDVVITGERSGR
                     RERFAVRADFPENRTDDYYIPRLWASRRVGDLTRQIHLEGASSDLVTRVRDLGLRYGI
                     LTDYTSYLVQEPDALASDPRGRPFLMEDESRTGGPESARRQTGRAAFEAAQSAAKLAR
                     ANSLDAAAEASPVPAPSHSQSGPASATRSASGRVFIRRGEVWTDAGHRDTVKVTAVAS
                     FSDAYFSLVRALPELAECLRLGDEVLIAGHRVSIRIGHSGIERWQSGELDQTVRDFRG
                     V"
     gene            4139..4642
                     /locus_tag="VFB89_07460"
     CDS             4139..4642
                     /locus_tag="VFB89_07460"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZM27177.1"
                     /translation="MTGDAGRLFEEYHDSLVRMLWRRTGDRDRAEELAQETFVRAVAA
                     QPRNPRSWLFAVALNLVREDARKALRQGRRLQLLKNELEPAAPSADEIMERDERAAKV
                     RAALEQLNERDREALLLKAEGFDYEEIAETLGLAKGAVGTTLARARRRLVEAYHDMEG
                     QAKHAAR"
     gene            4629..6083
                     /locus_tag="VFB89_07465"
     CDS             4629..6083
                     /locus_tag="VFB89_07465"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZM27178.1"
                     /translation="MPHVDEGTLHALLDGELGAAEVLEVQTHFATCAACAARLDEARQ
                     MLAETERLVAALEPPAGGGAAPAPRLVVGRPMIPVDPVVLIPNNPTPTEVRRSRFKIM
                     AWAASFLVIVGAGFLGLEMRNSLPGNHKPGSLRIRPEEFTFANDRTADSGGSRPGASK
                     PAPAAAADSLSQSRASSPQALPPSAPAPPSATPPAGRAQDKGAANQAAPTQPKTPPTP
                     AKPDASPSTAKDGTPVAELAAGAAAAKAVSGSPPGRAAPANAQKAAPAPAAASAKETA
                     GPQQPRDESKATDDEAAQPSQGQSAEDRAASVDRAMSDKATADLDREQMRQRAAAATA
                     ALDREREEERAAAAEEARRQEAAAAESSPATVDEKANVSRRIGLDEAARQLGGPLHAI
                     DGMTRQLVGLVSPKLVPGADTARAVVRAVYVDRNGRTLYLDQQRVRPGQLDLPFTPQV
                     GPNGQLSWMAGPVLLVLQSDLPQDSLRALARRVR"
     gene            complement(6133..>6813)
                     /locus_tag="VFB89_07470"
     CDS             complement(6133..>6813)
                     /locus_tag="VFB89_07470"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZM27179.1"
                     /translation="ALSWADVALIIGKAVVFLFGALSLGLWLSPRVFRLTARLRGRGI
                     LLATSLVFCLLLAWLAAEIGLAPIVGAYAAGLILERAHYRHFTQKGEHQLEELIHPIT
                     SFLVPVFFVLVGMGVDLSAFGNREVLGFAGLLTIAAVIGKQLCALGGVGARLDSISIG
                     IGMIPRGEVGLIFANIGLRLAINGEPVIDRGVYSAVVIMVMLTTLVTPPLLQWSLARG
                     AVRGGSRV"
BASE COUNT         1273 a         2181 c         2187 g         1172 t
ORIGIN      
        1 cgcttgggcc aacgcacgtg tccgcggctt tcgaacacca ggagcttcgt ggggccggca
       61 gccaggtcca ggcgccaccg gtgcgtgatg tttcggtgga tgatcacgta gtcgcccgcc
      121 cggaacggca ggtcgccgaa caccgattcg agaactcctg ttccctcaac tacataggcc
      181 acttcgtcgg ccatcgagtt tcggtagaaa tgagcgtcct gctcgtccgg ctcgacgtac
      241 aacattgcga tctcatgatt gaagaggatg ggaatccgat cgagggtcgg actaccgccc
      301 ttcttggccc tggcggtgag aaaatgccgg tgacgcagcg aggtgtcgtc gtcggcctcc
      361 cacacaattt ccttgacccg tcgtgccgac ttcaccgtag taggtgggtg cacatggtaa
      421 agcagggacg acgtgcccac gaatccttcg tggcccatca actcttcggc atagatccca
      481 ccgtccggct tacgaaagac agtatgccgc tttcgtggca tggagccgag ggaatggtag
      541 atcggcatta caacccccca aacgccgtaa acggtaaacg ggcccccccg gggcaccccg
      601 ttcaccgttt actgttcacc gttcacgctc ttatcaaagg tttcctctcg ctgcctgttc
      661 ccgctcgatc gcctcgaaca gcgccttgaa attccccgcg ccgaagctct tggcgccctt
      721 tctctgaatg atctcgaaaa agagcgtggg gcggtcctgg accggcttag tgaagatctg
      781 caggaggtag ccctcgtcgt cacggtcgac caggatgccc agctctcgga gtggcgcaat
      841 gtcttcgtcg atccggccga ccctatcgag cacggtatcg tagtacgtca agggcacgcg
      901 gagaaactcc actccccgat tttgcagcgc ggtgacagtg gcgacgatgt cgtcggtggc
      961 aatcgcgatg tgctgcacgc ccgggccgcg ataaaactcg agatactcat cgatctgcga
     1021 cttcttgcga ccctgcgccg gttcattcaa tggaaacttg atccgcccgt tgccgttggc
     1081 gacgactttg gacatcagcg cggagtactc ggtggagatc gtcttgtcgt cgaacgagat
     1141 caggttgtag aagccaagca cttgctcgta gtactggacc caccgattca tggcgcccag
     1201 ctcgacgttg cccacgcagt gatcgacata ttgcaggccc acggggggcg ggttgaaacg
     1261 ggagtccacc gccctgaatc caggtagaaa ggcgcctcga tagttacgac gctccaccag
     1321 cgaatgaatc gtctcgccgt acgtgtggat ccccgcgata acgacctcac cgtcatcgtc
     1381 cttgagcaca gtcggctcgt aggccggttt cgcgcctcgc tcgaccgcgt ttcggaaggc
     1441 cgttcgggtg tcgtccaccc agagggcgat gtctttcacg ccatcgccat gagcgttgac
     1501 gtgggcggca atcggatgat cgggccggag tgcggtcgtc agcaccagcc gaaccttgtc
     1561 ctgctcgagc gcatagctcg ccctgtcccg gacacccgtc tcgggccctc ggtacgccgt
     1621 gagccgatac ccaaacgcag cgcagtagta gagcgatgcc tgtttggcgt tgcctacata
     1681 aaactcaatg aaatcagtcc cgttgatcgg gaaggtgtcg gtggtggctg gagctgtgcc
     1741 gagcgcctcg gtgggcatgg cgcggtcctg ggtcgattga ctggggtaag gtagtcggga
     1801 gaaagaggga aggggagaaa gggagaaagg gataggcgtg caaggctgga ctgctgacgc
     1861 atctccccgc cgtcccgaac atcggagccg tcgatgtgcg cgtcacccag gacgttcgca
     1921 attctcaccg ccctcatccg gattaccccg tttgcctttc tccctttctc cttttctccc
     1981 gtgttcggtc agggctggat cgagatcgag aggcctgtcc ttcccgggcg gccactcggc
     2041 aacgtcgtcc ggaccagctc cgacgtgcgg gtcacggtga acgggcatgt cgcgcagatc
     2101 gcagtcgagg agcggttccg caacgacggc ggcggcctgg cggaagggag ctatctctac
     2161 ccgctcccct cggaagcggt ctttcagaac ttctcgctct ggaacggtga aactgagctc
     2221 aagggagaga tgctcaatgc cgaacaggcc cgccgggtgt acgaagaaat cgtgcgacgc
     2281 cgcaaggacc ctgccctgct aacgcttgcc ggccatcgtc tgatccgagc gcaggtcttt
     2341 cccattcagc cgggtgaaac ccggaaggtc gtgctgcgtt atacccagct gctcacccgc
     2401 gccggggacg cgctccgcct gcggtacgcc gccggcgatc gaggtggcac ttcggtgccc
     2461 ggcttccgcg tcacagtcga gaacgcggat caggtggggg agccctattc cccgacccat
     2521 cggctggaga cccgccgcga gggaaaccac ctccagatcg cgctcgcttc caacagcgct
     2581 ggcgatgtgg agctgttttt tcctctcgcg aaaggattgg tagcggccag cgtcgtaacc
     2641 catgcgcccg tgggcgagga tggctacttc ttgctgctaa tggcgccagg accggaaacc
     2701 cgggagacgt cgctgcctcg cgacgtcacg ctcgtagtgg atgtatcggg ctccatgtcg
     2761 ggcgagaaac tggagcaggc gcaggccggc cttcgccagg ctctcggcac gctcgccctc
     2821 caagaccgat tccggctgat cgctttttca tcgggtgtcc gcgagttcag gccgggcttt
     2881 acggctgcaa gccaggccgc catagactcc gcgcgacgat tcgttgatga tctcagcgcg
     2941 gatgggggga ccaacattgc cggggcgctg gacgcagccc tcgacgggac ggtcgaatcc
     3001 aaccggctgc ccatcgttct gttcctaaca gacgggcttc cctccatcgg cgagcaggcg
     3061 cccgatcgga tcgccgagcg ggcgagccaa cggctggcag gccgccggat cttcatggtg
     3121 ggcgtgggac acgacgtcaa cacctatctc ctagaccggc ttgccataga gggacgcgga
     3181 agtgtcgaat acgtagcacc gggctcgagc gtcgagaccg ccatcggcac tatcaccaca
     3241 cgaatccgtc atcccgcgct ggtcaatctg cggatcgaga gttcgcctgt ggggctgagt
     3301 gagttggcgc cgggccagct gccggatctg ttccagggag aggagttggt cgtgctgggg
     3361 cgctatcacg gtcaccgatc cggagacgtc gtcatcacgg gcgaacggag cgggcggcgc
     3421 gagcggttcg ccgtacgagc cgactttccg gaaaatcgga ctgacgacta ttacatccca
     3481 cggctctggg cctcccggcg agtcggcgat ctgacccgcc agatccacct cgaaggcgcc
     3541 agctctgatc tggtaaccag ggttcgcgac cttggactca ggtacggcat attgacggac
     3601 tacacgtcct atctggtgca ggagccggac gcgctggcgt ccgacccgcg tggcaggccc
     3661 ttcttgatgg aagacgagtc tcggacaggt ggtcctgaga gcgcccgccg gcaaacgggc
     3721 cgggctgcgt tcgaagcggc tcaatcggct gctaagctgg cacgggcaaa cagcctggac
     3781 gctgcagccg aggcgtcccc cgttcccgct ccgagtcact cgcagagtgg cccggcttct
     3841 gcaacccgca gcgcttctgg ccgggtcttt attcggcgcg gcgaagtatg gactgacgca
     3901 ggtcaccgag ataccgtcaa agtgacggcc gtggcatcat tcagtgatgc gtatttctcg
     3961 ttggtccgcg cgctgccgga attggccgag tgtcttcgct tgggcgacga agtgctgatc
     4021 gccgggcacc gggtgagcat ccggatcggg cacagcggca tcgagcggtg gcagagtggt
     4081 gagttggacc agaccgttcg cgatttccga ggagtctgag cgtgagcccg cgcgtggcat
     4141 gaccggggac gccggacgac tgttcgagga gtaccacgac tcgctggttc ggatgctctg
     4201 gcgccggacc ggcgatcgcg accgcgccga ggagctggcc caggaaacct tcgtgcgcgc
     4261 ggtggcagct cagccccgga atccccggtc atggcttttt gcggtcgcct tgaacctggt
     4321 ccgggaggac gcccgaaagg ccttacgcca gggaagacgg ctccagttgc tcaagaatga
     4381 gctcgagccg gcagcaccct cggcagacga aatcatggag cgggacgagc gcgcggccaa
     4441 ggttcgggcg gcgctcgagc aactcaatga gcgcgatcgg gaggctctgc tgctcaaggc
     4501 ggagggcttc gactacgagg agatcgccga aaccctgggc ctcgcgaagg gtgctgtggg
     4561 aacgacattg gcccgcgccc gacgccgcct ggtagaggcg tatcacgata tggaaggtca
     4621 agcgaagcat gccgcacgtt gacgaaggca cgctgcacgc gttgctggac ggggagctcg
     4681 gcgcggccga ggtgctggaa gtgcaaacgc atttcgccac ctgtgccgcc tgcgccgcgc
     4741 ggctggacga agctcggcaa atgttggcgg aaaccgagcg cctggtcgcc gctctcgagc
     4801 cgcccgcagg cggcggtgcc gcccctgctc cgcgactggt ggtcggacgt ccgatgattc
     4861 ccgtggatcc ggtcgtcctg atcccgaaca atccgacccc gaccgaagta cggcgcagtc
     4921 gcttcaagat catggcgtgg gccgcttcgt ttctggtgat tgtcggcgcc ggttttctcg
     4981 gcttggagat gcggaatagt ctccccggaa accataagcc aggttcgctc cgcatccgcc
     5041 cggaagaatt caccttcgcc aacgacagga ccgccgattc gggtggatcc cgcccgggcg
     5101 cgagcaagcc ggcgcccgcc gccgcggccg acagtttgag ccaatcgagg gcctcaagcc
     5161 cgcaggcgct ccctccctcc gctcctgcgc cgccgtcggc gactccgcct gctgggcgcg
     5221 ctcaggacaa aggtgctgca aaccaggcag cgccgactca accgaagact ccaccgaccc
     5281 cggccaagcc cgatgcatcg ccatctaccg ccaaggacgg aacaccggta gctgagctcg
     5341 cggccggcgc agcggcggcg aaagccgtga gtggctcacc gccgggacga gcagctcccg
     5401 ccaacgcgca gaaggcggcg ccagcacccg cggccgcttc agctaaggag accgccggcc
     5461 cgcagcagcc cagggacgag tcaaaagcga ccgacgacga ggccgctcaa ccctcccaag
     5521 gccagtcggc cgaggatcga gccgcatccg ttgatcgagc gatgtccgat aaggctaccg
     5581 ctgatctcga tcgcgagcag atgcggcagc gcgccgccgc ggcgaccgct gccctcgatc
     5641 gcgagcgcga agaggagcgc gctgccgccg cggaagaagc tcgccgtcag gaagccgcgg
     5701 ccgcggagtc gagccctgca accgtcgacg agaaggcaaa cgtctcacga cggatcggtc
     5761 tggatgaggc ggcacgccag ttgggcggcc ctttgcatgc aatcgatggg atgacccgcc
     5821 aactcgttgg actggtgtca ccgaagctcg tccccggagc ggacaccgcg cgggcggtgg
     5881 tgcgcgccgt ttatgtcgat cgaaatgggc ggacgctcta ccttgaccag cagcgggtgc
     5941 ggccggggca gctcgacttg ccgttcaccc cgcaagtcgg accaaacggt cagctttcct
     6001 ggatggcggg accagtgctg ctggtgctcc agagcgatct accacaggac tcgctgcggg
     6061 cactggctcg tcgcgttcgg tgagccggac agttcgatca ggtgttggta gactccacac
     6121 aagtaccagt tctcacacgc gactcccccc gcgaacggcg ccccgagcga ggctccactg
     6181 cagcagcggg ggcgtcacca gcgtcgtgag catcaccatg atcaccaccg ccgagtaaac
     6241 tccccgatcg atcacaggtt ccccgttgat ggcgaggcgc aggcctatgt tggcgaaaat
     6301 gaggccgact tcgccccggg gaatcatccc aatcccgatc gaaatcgaat ccaacctggc
     6361 cccgactcct cccaacgcac acaactgctt gccgatgacc gctgcaatgg tcagcaggcc
     6421 ggcaaagccg aggacctcac gattgccgaa ggccgacagg tccacaccca ttccgacgag
     6481 cacgaagaac acaggcacca gaaatgacgt gatcgggtgg atcagctcct ccagctgatg
     6541 ctcccccttc tgggtgaagt ggcgatagtg tgcccgctcg agaatgaggc cggccgcata
     6601 tgccccgacg atcggtgcaa ggccgatttc ggcggcgagc cacgcaagca ggaggcagaa
     6661 gaccaggctg gtggcaagga ggatgcccct tccgcgaaga cgggcggtga gccgaaatac
     6721 ccgcggtgac agccaaaggc ctagcgacag cgccccaaac agaaagacga ccgccttgcc
     6781 aatgattaag gccacgtcag cccacgacag ggc
//
LOCUS       DASRJQ010000337         2263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3961532, whole genome shotgun sequence.
ACCESSION   DASRJQ010000337 DASRJQ010000000
VERSION     DASRJQ010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3961532"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VFB89_07475"
     CDS             complement(<1..288)
                     /locus_tag="VFB89_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27180.1"
                     /translation="MKLLFFDDFRLGVLKGDAVVDVSDTVKDIPHTGPHNLISGLIER
                     FGDYKARLEGAAAKGQGKPVSQVRIRPPLPKPYTIVAMAVNYMEDGTRSEPA"
     gene            447..1226
                     /locus_tag="VFB89_07480"
     CDS             447..1226
                     /locus_tag="VFB89_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27181.1"
                     /translation="MIFTTFVLLIALGLFAAMVALLEVGRRIGIHRKRKDPEGAREGV
                     GAVEGAIFGLLGLLIAFTFSGAAARFDDRRHLIVEEANAIGTAYLRVDLVAPDLQPKL
                     RDSFKRYVDARLEVYRNVEDTTATQAALTEVGRLQQDIWSQAVAATRAPGSHPNLTML
                     LLPALNAMIDITTTRLMATRIHPPTIIFTMLLALALAGALLAGYGMAGAKTRSWTHVL
                     GFAAAMAVALYVILDLEYPRLGLIRVSDFDQVLVDLRATMK"
     gene            complement(1252..1920)
                     /locus_tag="VFB89_07485"
     CDS             complement(1252..1920)
                     /locus_tag="VFB89_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27182.1"
                     /translation="MLALIFGGMALSPPPAVAEETLAFDDKPIEKQPLDDKSAVEQQL
                     TLQETPAVSEKSKYPWSLTLIGGQYSGSQLLEIPARLALKDSWTLGLAVSKQFAEWTR
                     FLRWEGEFQVLKHIGEQDHWELTGSVNLRLVVFPWNRYVETTAAFGGGVSWASEVPAL
                     EKADSSNDTTNQWLNYLLLEITAGIPDSHWSLVTRIHHRSGIFGLFGHSGSNVLEAGI
                     RYRF"
     gene            2069..>2263
                     /locus_tag="VFB89_07490"
     CDS             2069..>2263
                     /locus_tag="VFB89_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27183.1"
                     /translation="MADRVQVSLRVPGTSPMPELIRLIQDIEAAGFDGAGILDSQMLC
                     RDTFVTLGQAASQTSRLTLFP"
BASE COUNT          379 a          752 c          712 g          420 t
ORIGIN      
        1 cgccggctcc gaacgggtgc cgtcttccat gtagttcacg gccatcgcga cgatggtgta
       61 cggcttgggc agcggcggcc tgatccggac ctggctgacc ggcttgccct ggcccttcgc
      121 agcggcgccc tccaggcgag ccttgtagtc gccgaaccgc tcgatcagcc cgctgatcag
      181 gttgtgcggt ccggtgtgcg gaatgtcctt taccgtgtcg gatacgtcga ccacggcgtc
      241 gcccttgagc acccccagcc tgaagtcatc gaagaagagc agcttcatga tcccctcccg
      301 cggattggtc tggcgccagg actataacag cgttcgcgcc gatgggccgg aggtcctggc
      361 ctggccgtct cccgcccttg gtctcatccc ggtaccaggc aacgcagtgt acgctctcgt
      421 ggccggggag gccaaatgga acgccaatga tcttcacgac gttcgtgctg ctgatcgcgc
      481 tggggctctt cgctgcgatg gtcgcgcttc tcgaggtcgg ccgtcgtatc ggcatccacc
      541 gcaaacggaa agatcccgag ggagcccgcg agggcgtcgg cgcggtcgag ggcgcgatct
      601 tcgggctcct cgggctgctc atcgcgttca ccttctcggg agcggcggcg cgcttcgacg
      661 atcgccggca cttgatcgtc gaggaggcca acgcgatcgg gacggcctat ttgcgagtcg
      721 acctggttgc gcccgaccta cagccgaagc ttcgagacag cttcaagcgc tacgtcgacg
      781 cgcggctcga ggtctatcgc aacgtcgagg acacgaccgc gacgcaggcg gcgctcaccg
      841 aagtggggcg cctgcagcag gacatctgga gtcaggccgt cgcggccacc cgcgccccgg
      901 gctcccaccc caatctcacg atgctgctct taccggcact gaacgcgatg atcgacatca
      961 cgacgacccg gctgatggcc acgcgcattc acccgccgac gatcatcttc acgatgctct
     1021 tggccctggc cctggccggc gcgctgctgg ccggttatgg catggccgga gccaagacgc
     1081 gtagctggac gcacgtcctg ggcttcgcgg ccgcgatggc cgtggccctc tacgtcattc
     1141 tcgacctcga gtacccgcgg ctcggtctca tccgcgtctc ggacttcgac caggtcctcg
     1201 tagatctgcg cgcgacgatg aagtgagatc gagccgccgc gcgaccggcc gtcagaatcg
     1261 gtagcggatg ccagcctcca gaacgttcga gccgctgtgc ccgaataggc cgaagatgcc
     1321 ggatcggtga tggatgcggg tgaccaggct ccagtgcgaa tccgggatgc cggccgtgat
     1381 ctcgagcaga aggtagttca gccactggtt ggtcgtgtcg ttcgacgagt cggctttctc
     1441 gagtgccggg acctcgctcg cccacgaaac gccgccgccg aatgccgcgg tcgtctcgac
     1501 gtagcgattc cacggaaaca ctaccagccg gaggttgacc gagccggtca attcccagtg
     1561 atcctgctcg ccgatgtgct tgaggacctg aaactcgccc tcccatcgca agaatcgcgt
     1621 ccattccgcg aactgcttcg agaccgcgag tcccagcgtc cacgaatctt tgagagcgag
     1681 tcgggcgggg atctctagaa gctggctgcc ggagtactgg ccgccgatca aggtcaggga
     1741 ccacgggtat ttcgattttt cggaaaccgc cggcgtctcc tggagtgtca gctgttgctc
     1801 gaccgccgac ttgtcgtcga gcggttgctt ctcgatcggc ttgtcgtcga atgcgagtgt
     1861 ttcttcggcc actgccggtg gcggagagag cgccatcccg ccgaaaatga gcgcgagcac
     1921 gagaacaata cgggaatcga cactccgcat cccagagtgg ccccgcgcat cctagcagaa
     1981 gccatcacgg cgaacgtgct ctaccttggt ccaacccgtc tggacaagcc cgaatcgaca
     2041 ccctatcctc cctggcgagg aggatggcat ggccgatcgt gttcaggtct cactgcgcgt
     2101 tcccggcacc tcgccgatgc cggagctgat ccggttgatt caggacatcg aggcggccgg
     2161 attcgacggc gcgggcattc tcgacagtca gatgctctgc cgtgacacgt tcgtcacact
     2221 cggccaagcg gccagccaga cctcgcggct cacgttgttt ccc
//
LOCUS       DASRJQ010000338         3652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2034658, whole genome shotgun sequence.
ACCESSION   DASRJQ010000338 DASRJQ010000000
VERSION     DASRJQ010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2034658"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VFB89_07495"
     CDS             <1..403
                     /locus_tag="VFB89_07495"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SURF1 family cytochrome oxidase biogenesis
                     protein"
                     /protein_id="HZM27184.1"
                     /translation="AGDTAVLVERGFVPSPDAVTLPPAAARLDEPGQQLVHGVATALT
                     AKADSGAPLVHDGATTWKRLDLGALRARLPYPILSVVIRQTPDSSLPQMPRRREAAAL
                     DEGPHLSYAIQWFAFATIAVVFAGVFWRAGR"
     gene            complement(411..1625)
                     /locus_tag="VFB89_07500"
     CDS             complement(411..1625)
                     /locus_tag="VFB89_07500"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HZM27185.1"
                     /translation="MSAQTSSSPDLARVHKILQSVPLIDGHNDLPWRIRAEVNPPRDV
                     AAYDLRQQTSGHTDLARLKAGQVGAQFWSVYIPGEIKESGYARVQLEQIDIARRVIAR
                     YPDRLALALTAADIEREFKRHRIASLIGMEGGHAIENSLGALRVYYSLGARYMTLTHN
                     VTTDWADAALDSARHGGLTPFGREVVHEMNRLGMLVDLSHVSPATMSAALDVTEAPVI
                     FSHSSARALVDHPRNVPDSILRRLGANGGMVMVTFVPAFVSAEAAAVDLRRTAREKEL
                     KLTVADTAERRKALEAWDRENPQPRATLAQVADHIDHIRQVAGSDHVGIGSDFDGIDH
                     VPIGLEDVSHFPDLFAELARRGWSDDDLKKLAGRNLLRVLHQAETVAQRLQREREPST
                     KTIEELDHTDSR"
     gene            complement(2105..>3652)
                     /locus_tag="VFB89_07505"
     CDS             complement(2105..>3652)
                     /locus_tag="VFB89_07505"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZM27186.1"
                     /translation="AALALPVVPPSSTAPPRPRLVVLIAVDQLRGDYFKRFEPQLSGG
                     FARFWRDAAFFEQGRQDHAVTETAPGHSTMLSGRWPAHTGIFNNLFGVTDPTTPLLEL
                     KDSGQGASPRNFRGTTLYDWLRASDSASRVLSVSRKDRGAILPVGRSRGDVYWFDHGI
                     FTTSTWYRTEDTLPGWVREFNAADPVRKLAGSTWSLLLPDSAYTEVDSVPFEAGGGPI
                     TFPHQLSPNVDAAVLQIVAFPVMDSLTLALALRGVERLRLGQGRATDLLVVSLSTTDA
                     VGHAWGPDSRELHDQVLRADRLLGRFMDSLATLVPREATIWALTSDHGITSMPELASS
                     KGVRAGRVWPTDALNELAQAVGDNQSFGFEDGLLFANVTRLRSRGVNLDSLATAMAAK
                     VKALPGVSQVYTPKTLRSASGSDTGAVLWRRTIPDDIGWLLCATLESGFVWSPGRVIA
                     EHGSTSLNDQWVPIAFMGPGFVSHHFTEPVSTTDIAPTLAKVLSVKPSEKLDGHALTE
                     ALAGGGR"
BASE COUNT          698 a         1150 c         1173 g          631 t
ORIGIN      
        1 tgcgggcgat accgccgtcc tggtcgagcg tggctttgtg ccgtcgccgg atgccgtcac
       61 gcttccaccg gcagcggcgc ggctggatga gcccggacag cagctggtgc atggcgtcgc
      121 aacggcgctc acagcgaagg cggatagcgg agcaccactg gtccatgacg gagcaaccac
      181 ctggaagcgc ctcgacctgg gcgccttgcg tgcccgcctt ccctacccaa tcctgagcgt
      241 ggtgattcgc caaacccccg acagctcact gcctcagatg cctcgccgcc gagaggctgc
      301 ggcgctggat gaaggacccc acctgagcta cgcgattcag tggttcgcgt ttgcgacgat
      361 cgcagtggta tttgcaggcg tgttctggcg ggccggtagg tagctatcag ctaacggcta
      421 tcagtgtggt cgagctcctc gatggttttc gtagagggtt cccgctcgcg ctgaagccgc
      481 tgagccaccg tctccgcctg atgcagaact cgcagcagat tgcgtcccgc gagcttcttc
      541 agatcgtcgt cactccagcc gcgacgggcc agctcggcga acagatcggg gaagtgggag
      601 acgtcctcga gaccaatggg gacgtggtcg atcccgtcga aatcacttcc gatcccgaca
      661 tggtcgctcc ctgcgacctg ccggatgtgg tcgatgtggt ccgctacctg cgccaacgta
      721 gcccgaggtt gagggttctc acgatcccag gcttcgagtg ccttccgacg ctccgccgtg
      781 tcggcgaccg tgagcttgag ctccttttcc ctcgccgtgc ggcgcaggtc taccgccgcc
      841 gcctcagccg agacgaaggc cgggacgaac gtgaccatga ccatgccccc attggcaccg
      901 agtctccgga gaatcgaatc gggcacattg cgcggatgat ccaccaacgc acgcgccgag
      961 gagtgcgaga agatcaccgg cgcctcggta acatcgagcg cggcgctcat ggtggccggc
     1021 gagacgtggg acaggtcgac cagcatcccc aggcggttca tctcatgcac cacttcgcgg
     1081 ccgaacggcg ttagtccacc gtggcgtgcc gaatcgaggg cagcatcggc ccagtccgtc
     1141 gtgacgttgt gcgtcagggt catgtaccgg gcgccgaggc tgtaatagac gcggagcgcg
     1201 ccgagggagt tctcgatcgc atggccacct tccataccga tcaatgaagc gatgcgatgt
     1261 cgcttgaact cgcgttcgat gtccgccgcc gtgagcgcga gtgcaaggcg gtccgggtaa
     1321 cgagcgatga cccgccgcgc aatgtcgatc tgctccagtt gtaccctcgc gtatccgctc
     1381 tccttgatct caccggggat gtagaccgac cagaactggg cgccaacctg gcctgccttc
     1441 agccgcgcca gatccgtatg gccactggtc tgctgccgca gatcgtaggc cgcgacgtcc
     1501 cggggagggt ttacctcggc ccggattcgc cacggcagat cgttgtggcc atcgatgaga
     1561 ggaaccgatt gaagaatctt gtgaacgcgg gcgaggtccg gagacgagga ggtttgcgca
     1621 gacatggcag aagcgaaaag cgtgaacggt gaacagtgaa cagtgaacag gaagagcagc
     1681 cgaccagcat cgcgaagcat gaaacgccaa gcccgttcac cgttcactgt tcaccgttca
     1741 cgcctttctc aagttcacgc ttttccgagt tggaccaact caggatgggt cacgaactcc
     1801 gggcaaatgg tccgatcaaa ctcataaggt ccaccggtct ccgcgtcggt ccaagggaag
     1861 ccgcgacaga ccgcggggta gtagccctgt ccatggatgg tgcagcgatt ctcaaccagg
     1921 aacacgcaac gccgattgcg aacacgagtt cgccattcgc cccagcgggt ccggtagatc
     1981 atgtcggcac cgtgagcacg cgtgatagcg tgcgcctccg tgtcactcag ggtaacgcca
     2041 aaggcgcagc aagcggattg gtggggacag ggaaagcacg gaagcggcgg atacaccgac
     2101 tccgtcaccg accgcctccc gccagcgcct cggtgagagc gtgtccatcg agcttttcgg
     2161 atggcttcac actgagcacc tttgccagcg ttggcgcaat gtcggtggtg ctcaccggct
     2221 ccgtgaagtg gtgcgacaca aatcccggac ccatgaatgc aatcggtacc cactgatcgt
     2281 tgaggcttgt gctaccgtgc tcagcgatca cacgtccggg tgaccacacg aatccggatt
     2341 cgagcgtggc acagagaagc cagccgatat cgtccgggat cgtacggcgc cagagcacag
     2401 ctccggtatc agacccggac gcgctgcgca aggtcttggg cgtgtagact tggctgaccc
     2461 cgggaagcgc tttcactttc gctgccatcg cagtggcaag gctgtccagg ttcacgcccc
     2521 gggaacgcag ccgagtgacg ttggcgaaca gcagtccgtc ctcgaagccg aaggactgat
     2581 tatcgccgac ggcttgtgcc aactcgttca gagcatcggt gggccagact cgaccggcgc
     2641 gcactccctt gctgctggcg agctcaggca tcgaagtgat cccgtgatca ctggtcaggg
     2701 cccaaatggt cgcctcgcgt ggaaccagtg ttgcgagcga gtccataaac cgccccagca
     2761 ggcgatcggc ccggagcacc tgatcgtgca gctcgcgcga gtcgggtccc caggcatgcc
     2821 cgacggcatc cgtggtggag agggacacaa ccaggagatc cgtggctcgt ccttggccga
     2881 gtcgcaggcg ctcaacgccc cggagcgcaa gggcgagggt gagcgagtcc atcaccggga
     2941 aggcgactat ctggagaact gccgcgtcca cgttgggcga aagctggtgg ggaaacgtta
     3001 tcggtccgcc ccccgcttcg aacggcacgc tatcgacctc ggtgtacgcc gaatccggca
     3061 atagcagcga ccacgtcgag ccggccagct tgcgcaccgg atctgcagcg ttgaactccc
     3121 ggacccatcc cggcaacgtg tcctcggtgc ggtaccaggt gctggtcgtg aaaatgccgt
     3181 ggtcgaacca gtacacgtca ccgcggctcc ggccgactgg caggatggcg ccgcgatcct
     3241 tacgtgagac agaaagcact cggctggcag aatcggaggc gcggagccag tcgtacagcg
     3301 tcgtgccacg gaaatttcga ggtgatgcac cctgcccgga gtctttgagc tcgagcaacg
     3361 gcgttgtcgg gtccgtgaca ccgaacaggt tattgaaaat tccggtgtgg gccggccagc
     3421 ggcctgacag catggtcgaa tgcccgggtg ccgtctccgt cacggcgtgg tcctgccgtc
     3481 cctgttcgaa gaaggcagca tcgcgccaga accgggcgaa cccacccgac agctgaggct
     3541 cgaaccgttt gaagtagtcg ccccgcagct ggtccacggc gatcagcaca accaggcgcg
     3601 gtcgtggtgg ggctgtcgaa gacgggggaa caacaggtag cgccagcgcg gc
//
LOCUS       DASRJQ010000339         2146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_943043, whole genome shotgun sequence.
ACCESSION   DASRJQ010000339 DASRJQ010000000
VERSION     DASRJQ010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943043"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..645
                     /locus_tag="VFB89_07510"
     CDS             160..645
                     /locus_tag="VFB89_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27187.1"
                     /translation="MAVSSVGAKVTPEAQLRALIEKLDPKDQKLFRSVRSAVRKRLPA
                     ANELVYDYTGNLVIGYSPSDRGIEGIVAISARPDDVRLYLTQAPRLPDPKKLLMGSGK
                     QARYIPVEAASRLAHPDVEALIAAAIDQASVPLPSKGRGTVIIRSGAEKKKQPRRRPA
                     K"
     gene            642..1322
                     /locus_tag="VFB89_07515"
     CDS             642..1322
                     /locus_tag="VFB89_07515"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HZM27188.1"
                     /translation="MSADVARAPRRLIDVSHTVEHGMTTYKGFPAPIICDWLSREASR
                     AKYAPGTEFHIGKIEMIANTGTYIDSPFHRYEDGKDLSQLPLESLADLECLVVRVDPA
                     AGPAIDRIPLAASQVGGRAVLFHTGWDRHWRSEAYFEGHPYLTRRAAEWLAKAGAAIV
                     GIDSLNIDSVATGERPVHTVLLGEGIPIVEHMCGLADLPDEGSRFFAVPVKVKAFGTF
                     PVRAFALA"
     gene            1330..>2146
                     /gene="bla"
                     /locus_tag="VFB89_07520"
     CDS             1330..>2146
                     /gene="bla"
                     /locus_tag="VFB89_07520"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF012229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallo-beta-lactamase"
                     /protein_id="HZM27189.1"
                     /translation="MPHFTKRTTLVVFVGLASVLALVLVGRWVNATKRGGQEPAEPFR
                     IAGNFYYVGATDAAAFLITGPEGHIVLDGGYPSTAPMIIASIAKLGFDIKDVKVLLNS
                     EPHPDHGGGLGVLQQASGAELWASEASASTLASGGDDPDVVLPLRALVWIGVFGYPKM
                     RVDHRFNDGDTIRLGPLALTAHLTGGHTRGCTSWSFQVRDGDRLLNVVSACDLGRLAT
                     SQYPEQQADLERSFSVLRGLPVDIWVTCHARWWGRYRKFVASAAAKNAVEPFID"
BASE COUNT          361 a          681 c          727 g          377 t
ORIGIN      
        1 ggccacggtg ctgttcctga tcgcgttggc ccagcgattc acgatgcgcg gggtgcggat
       61 gggactccta tcggtggcaa ccctcctcat ggggtacggg ctcatcacgt tggcgacgta
      121 cccccgcttg tagatcttta ccagaaactg gaggacgaga tggcagtcag ctcagttggc
      181 gcgaaggtca caccggaggc ccaacttcgg gccttgatcg agaagctcga tcccaaggac
      241 cagaagctct tccgctccgt ccggtcggct gtgcgcaagc gattgccggc tgccaacgag
      301 ctggtctacg actacaccgg caacttggtg atcgggtatt cgccgtcgga tcgagggatc
      361 gagggaatcg tcgcgatctc cgcgcgtccg gacgatgtgc gtctgtactt gacgcaagca
      421 cctcgattgc ccgacccgaa gaagctcttg atggggtcgg gcaagcaggc ccgctacatc
      481 ccggtggagg cagcgagccg cttggcgcat ccggacgtcg aggcgctgat cgccgccgcg
      541 atcgaccagg cgagcgtccc gttgccatcc aaggggcgcg gcacggtcat cattcggtcg
      601 ggtgctgaga agaagaagca gcctcgtcgg aggccggcga agtgagcgcg gacgttgcca
      661 gggcgccgcg acgtttgatc gacgttagtc acaccgtgga gcacgggatg acgacctaca
      721 agggctttcc ggcgccgatc atctgcgatt ggctcagccg cgaggcgtcg cgagcgaagt
      781 acgcacccgg caccgagttc cacatcggca agatcgagat gatcgccaac acgggcacgt
      841 acatcgacag cccatttcac cgctacgagg acggaaagga tctttcccag cttccgctcg
      901 agtccctcgc cgatctcgag tgcctcgtgg tgcgcgtcga tcccgcagcc gggccggcga
      961 tcgatcggat acccctggcg gcgagccagg tcggcggccg cgcggtgttg ttccacaccg
     1021 gctgggatcg gcattggcgc agcgaggcgt acttcgaggg gcacccgtac ctgacccggc
     1081 gtgctgcgga gtggctcgcc aaggccggcg ccgccatcgt ggggatcgac tcgctgaaca
     1141 tcgacagcgt ggccaccggc gaacgaccgg tgcacacggt cttgttgggt gaggggatac
     1201 cgatcgtcga gcacatgtgc ggtttggccg acctcccgga tgaagggagc cggttcttcg
     1261 ccgtgccggt caaggtgaag gcgttcggga cgtttcctgt gcgagcattc gcgctggcgt
     1321 aggtgacggt tgccccactt caccaagcga accacgttgg tcgtcttcgt gggactggcg
     1381 agtgttcttg cgctggtcct cgtcggcagg tgggtgaacg cgaccaagcg gggtgggcag
     1441 gagcccgccg aaccgtttcg catcgccggc aacttctact acgttggcgc gacggacgcc
     1501 gcggccttcc tcatcaccgg gcccgagggc cacatcgtgc tcgatggcgg ctatccgagc
     1561 accgcgccga tgatcatcgc gagcatcgcg aagctcggct tcgacatcaa ggacgtcaag
     1621 gtgctcctga actcggagcc tcaccccgac catggcggcg ggctgggcgt gctccagcag
     1681 gcgtccggtg ccgagctgtg ggcgagcgag gcgagtgcct cgaccctcgc ctcgggtggc
     1741 gacgacccgg acgtagtcct gccgctccgg gccctcgtgt ggatcggggt cttcggctat
     1801 ccgaagatgc gggtcgacca ccggttcaac gacggcgaca cgatccgcct cgggccgctc
     1861 gcactcaccg cgcatctcac cggcggccac acgcgcggat gcacctcctg gtcgttccag
     1921 gttcgtgacg gggatcgcct gttgaacgtc gtgagcgcgt gcgacttggg gcggttggcc
     1981 acatcgcagt atccagagca acaggccgac ctcgaacgca gcttcagcgt gctccgaggc
     2041 ctccccgtcg acatctgggt gacgtgccac gcgagatggt ggggcagata tcgcaagttc
     2101 gtcgctagcg ctgctgcgaa gaacgctgtg gaaccgttca tcgacc
//
LOCUS       DASRJQ010000340         3234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_569585, whole genome shotgun sequence.
ACCESSION   DASRJQ010000340 DASRJQ010000000
VERSION     DASRJQ010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569585"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1619
                     /locus_tag="VFB89_07525"
     CDS             <1..1619
                     /locus_tag="VFB89_07525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZM27190.1"
                     /translation="PPGDSGLTRMAYLREVLGPQIRQYHADLAGAGAAVLHPAEKALQ
                     ESQRLLELFFAQSLDGFFFMMLEEPVRWDDTVDKDAVLQFVFEHQRVTRVNDAMARQY
                     GAGPEDLLGRTPAELFVHNPMYGKNLWRQLFDAGRLHVETDERKLDGTPIWIEGDYIC
                     LYAPDGRIMGHFGIQRDITARRQAEEALRFSEEKFAKAFRSSPFRVTIGTLAEGRFIE
                     VNDAFLRDHGFTREQVIGRTSPELGLWDRPEDRRRLVEALERDGKVHEFEYPGQTRDG
                     RVHITSLSAELIQVAGETCILAVATDITDRKRAQEEIRRSREELRALAARLQSVREDE
                     RTHIAREIHDELGQALTGLKLDLAWLRQRLKDRTDLTERLASVMGRIDGTVDAVRRIA
                     TELRPSVLDHLGLVAAVEWQAQEFERLTGVSTRLDVSATTIPVNGTTATTVFRMLQET
                     LTNVARHAAATRVEITLEIGSEAVSFRVTDNGRGISDAERTDRRSLGLVGLRERAIAS
                     GGQLSIEGRAGAGTVVSARIPTNPTGEFML"
     gene            1616..2248
                     /locus_tag="VFB89_07530"
     CDS             1616..2248
                     /locus_tag="VFB89_07530"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZM27191.1"
                     /translation="MIRVFIADDHPVVRAGLRGIIEGEPGLEVVGEAINAGDLLGTIG
                     RTPAEVLLLDVSMPGPGFLEVLRDIKRDHPRVAVLVLSVHPEDQYAVRALRSGAAGYL
                     TKDHSPEELVAAIRKVHRGGKYVSPSLAEKLAAGLEIGAAGAAHETLSDREYDVLCLL
                     GSGQTVKEIAGGLGLSPKTVSTYRARILEKMKLATNADLVRYAAQHGLIR"
     gene            2292..3143
                     /locus_tag="VFB89_07535"
     CDS             2292..3143
                     /locus_tag="VFB89_07535"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZM27192.1"
                     /translation="MTHAIEVERRKDDYAMGRTPAEYERLRQQARILEPAARRVLERI
                     GLASGMCCVDVGCGPGEVMRLMGELVGPSGRVVGLDVDSTLGAQALAMLREGGTCSFE
                     FVEADAAKLDQLPGGACDLAYARLVLIHAQDPAVLLRRMYGWLRPGGYLVVQDFDLKG
                     MDVDAAGEAGREFLRVGLGVFQALGLDPQAGQNLPYYFRTAGLGRPDGTEISGILSPM
                     TTVAPMMEGVFRSVLPHALRLGITTEARSEMLLAGLAGLAARDDMWGRWPLLVSAWKR
                     KPLEASD"
BASE COUNT          566 a          993 c         1108 g          567 t
ORIGIN      
        1 cgccaccggg tgattcgggt ctcacccgga tggcgtacct gcgtgaagtg ctcggccccc
       61 agattcggca gtaccacgcc gaccttgcgg gtgccggagc ggccgtcctc catccggccg
      121 agaaggcgtt gcaggaaagc cagcgtctgc tcgagctgtt cttcgcccag tcgctggacg
      181 gcttcttctt catgatgctg gaagagccgg tccgctggga cgacactgtc gacaaagacg
      241 ccgttctgca gttcgtgttc gagcaccagc gggtcacccg agtcaacgac gccatggcgc
      301 ggcagtacgg cgccggcccg gaagacctct tggggcggac gccggctgag ctgttcgttc
      361 acaatccgat gtatggaaaa aatctgtggc gccagctctt cgatgccggc cgcctccacg
      421 ttgaaactga tgagcgtaag ctcgacggca cgcccatctg gatcgagggg gattacatct
      481 gcctctacgc tcccgacggc cgaatcatgg ggcactttgg catccagcgg gacatcaccg
      541 ctcgccgcca ggcggaggaa gcgctgcggt tttcggagga gaagttcgcc aaggcgttcc
      601 gctcgtcccc cttccgggtg accattggaa cactcgcgga ggggcgcttc atcgaggtga
      661 acgacgcgtt tctgcgcgat cacggcttca ctcgtgagca ggtgattggc cggacctcgc
      721 ccgagttggg gctctgggac aggccggaag accgcaggcg attggtggaa gcgctcgagc
      781 gcgacggcaa ggtccacgag ttcgaatacc ccggccagac ccgtgacggc cgggttcaca
      841 tcacctcgct gtccgccgag ctgattcaag tggccggtga gacctgcatc cttgcggtcg
      901 ccaccgacat caccgaccgg aagcgggcgc aggaggaaat ccggcgctcc cgcgaggagc
      961 tgcgggcgct ggctgcccgg cttcagtcgg tgcgcgagga cgaacgaact cacattgcga
     1021 gggagatcca cgacgagctg gggcaggcgc tcaccggtct caagctcgac ctggcgtggc
     1081 tccggcagcg gctcaaggac cggaccgatc tgaccgagcg actggcctcg gtgatgggcc
     1141 gaatcgatgg taccgtcgac gccgtacgtc ggatcgccac cgagctgcga ccttcggtgc
     1201 tcgaccacct cggtctggtc gcggcagtgg aatggcaggc tcaggagttc gagaggctaa
     1261 ccggcgtctc gactcgactg gacgtaagtg ccacgaccat acccgtgaac ggcacaacag
     1321 ctacgacggt gttccggatg ctgcaggaga cgctgaccaa cgtcgccagg cacgcagccg
     1381 ccacccgggt cgagatcacg ctcgagatcg gctccgaggc agtgtcgttc cgcgtcaccg
     1441 acaacggacg gggaatcagc gatgcggagc gcaccgaccg ccggtcgctg ggactggtcg
     1501 gccttcgcga gagggcgatt gcctcaggcg gtcagttgag catcgagggg cgtgccggag
     1561 cggggaccgt ggtctcggcc cgaattccca ccaatccaac cggcgagttc atgctgtgat
     1621 cagggttttc atagccgacg accacccggt ggttcgtgcg ggcctgcgag gtatcatcga
     1681 aggcgagccc ggcctcgagg tggttggcga agcaatcaat gccggcgacc tgctgggaac
     1741 aatcggacgg accccggctg aagtgttgtt gctcgacgta tcgatgcccg gtccggggtt
     1801 tctggaagtg ttgcgcgaca tcaagcggga ccatccccgc gttgccgtgc tggtgctcag
     1861 cgtgcatccg gaagaccagt atgcagtccg ggccttacgg tcgggcgccg caggctatct
     1921 caccaaggat cactcacccg aggagctggt cgccgccatc cgcaaggtgc accgcggggg
     1981 caagtacgtg agtccttcgc tggcggagaa gctggcggcg ggtctcgaga tcggtgcggc
     2041 cggtgctgct catgaaacgt tgtcggatcg cgagtacgac gtcctttgcc tgctgggctc
     2101 agggcagacg gtgaaggaaa tcgcgggtgg tctgggtttg agtccgaaaa cggtgagtac
     2161 ctatcgcgcg cggatcttgg agaagatgaa gctggcgaca aacgccgacc tggtgcgcta
     2221 cgctgcccag cacgggttga tacgctgaga gcaatgacgg ccggccacgg ttggccggcc
     2281 cggaggctag ggtgacacac gcaatcgaag tcgagcgccg caaagacgac tatgccatgg
     2341 gccggacgcc ggccgagtac gaacggctcc gtcagcaggc tcgaatcctg gaaccggcgg
     2401 cccgtcgagt gctggagcgg ataggcctcg cctcgggaat gtgctgcgtc gacgtcggct
     2461 gcgggcccgg tgaagtcatg cggctcatgg gagagctggt gggaccgtcg ggtcgcgtgg
     2521 ttggcctcga tgtcgactcg acgttgggcg cccaggcgct cgcaatgctg cgggaggggg
     2581 gaacctgcag cttcgagttc gttgaagccg atgctgcaaa gctggaccag ctgccgggag
     2641 gcgcatgtga cctcgcctat gcccgcctgg tactgatcca tgcacaggat ccagcggttc
     2701 tgctccgccg catgtatggc tggctgcgac cgggaggcta tctcgtcgtt caggacttcg
     2761 acctgaaggg aatggacgtc gatgcggccg gcgaagccgg gcgggagttc ctgcgagtgg
     2821 gactcggggt gtttcaggcg cttggccttg acccgcaagc cggtcagaac cttccctact
     2881 atttccgcac ggctggcctg ggaaggcccg acggcaccga aatcagcggc atcttgagtc
     2941 caatgacaac agtggccccc atgatggaag gtgtgttccg cagcgtgctt ccgcacgcgc
     3001 tccgtctcgg tatcaccacg gaggcccgca gcgaaatgct gcttgcaggc ctggccggcc
     3061 tggccgcgcg cgacgacatg tggggccgct ggccgctgtt ggtttcggcc tggaagcgga
     3121 agccgctgga agcttccgat tgaggcccgc cattcggttt attgatcgaa ccggtacacc
     3181 gtccctccct tcattaccca ccggacccgc tccagctctg taatgtctgc gagc
//
LOCUS       DASRJQ010000341         8390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_879170, whole genome shotgun sequence.
ACCESSION   DASRJQ010000341 DASRJQ010000000
VERSION     DASRJQ010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 8390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8390
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879170"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /gene="mltG"
                     /locus_tag="VFB89_07540"
     CDS             <1..376
                     /gene="mltG"
                     /locus_tag="VFB89_07540"
                     /inference="COORDINATES: protein motif:HMM:NF014656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HZM27193.1"
                     /translation="GVYRNRLRIHMALQADPTVQYAILLANGIRKPRLYQKDYLTPSP
                     YNTYLHPGLPPGPVNSPGRRSIEAALYPADVHYLYFVAGPDGRHVFSRTYDEHLRNVG
                     KMRRLMKTPSGEQGDKSVNGKR"
     gene            complement(467..1066)
                     /gene="thiE"
                     /locus_tag="VFB89_07545"
     CDS             complement(467..1066)
                     /gene="thiE"
                     /locus_tag="VFB89_07545"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HZM27194.1"
                     /translation="MLVTDDALLRERDLLDVGRAAEQGGVTAVQVRLKHATPRELVDA
                     TRTLVAVLRIPVLVNDRIDAALASGAAGVHLGPDDLALPLARRIAPPGFIVGASAGTV
                     EEAEAARGADYWGVGPWRASGTKPDAGVALGAEGFQKLVAAGNGIPCIAIGGVRPEDV
                     AAVLSAGGAGVAVVSGILGADDVESAARRYWKSVNGERY"
     gene            1098..1475
                     /gene="aroH"
                     /locus_tag="VFB89_07550"
     CDS             1098..1475
                     /gene="aroH"
                     /locus_tag="VFB89_07550"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF019356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HZM27195.1"
                     /translation="MPEIPMPRIHGIRGATTVERNDAEEILGATDELLRALISANDLQ
                     PDDIVSAFFTVTNDLDAAFPARAAEDFGWNIVALLHATEIPVPGSLPKCIRLLVHAYT
                     TRSRGEIKHCYLRRAISLRPDRA"
     gene            1472..2647
                     /locus_tag="VFB89_07555"
     CDS             1472..2647
                     /locus_tag="VFB89_07555"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HZM27196.1"
                     /translation="MTDIRVTARGAERWRRGHPWIHRTELRDEPGQPGLVNVRDPRGK
                     FIGQALYSPSSEIRLRLLEPSDQPVDAKWWRHQVASALARRKSIDATAYRVVHAEADG
                     LPSLVVDRYDRWVVAQLLSGGLESQREQVIAALLDVLRPDGLLLRHDAPARRRERLPQ
                     ETVLAYGKVPEWIEVREANVRYLAAPWTGQKTGAFLDQRPNRILAAALTPQGSRTLDC
                     FAYHGSFALHLATRSAEVIALDSSAEALVRGAANAELNGFTNIVWREADAFQTLRAFE
                     RNRDRFDTIVLDPPAFAKTRSAVSQAMRGYHEINLRAMRLLAPSGHLVTASCSFHVHR
                     AEFLAMLASAAADSGRRLELRALVGPGEDHPEILTIPETGYLKGAVVRAGGESALKR"
     gene            2757..3620
                     /locus_tag="VFB89_07560"
     CDS             2757..3620
                     /locus_tag="VFB89_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27197.1"
                     /translation="MLTAAQALRLDRRRRPRPSTKRQYDEYILQKIEAYKNSLSRDQL
                     LLLGDEANQELLAATEGQFVLTEILMLDSVDDLIKRRLKLKPFKKWKAWIKDLRDAQR
                     EPTHWGLEPECPVAGLVRRVEPQDEAVVVGGAADGAAFLLAAHEIPVTFLAADLGSVD
                     RVESRVEDESLAVPVIATVVDFELWCPPLPDSIAITVVDAGEVGELDPVVRAGLINAL
                     QSRTCEAGVHVLLPGRGGMAPEALLHHYDGWIREADGRPKRRGGRSAGIVLSWAPAKP
                     SALPADDAAAC"
     gene            3743..4783
                     /gene="tdh"
                     /locus_tag="VFB89_07565"
     CDS             3743..4783
                     /gene="tdh"
                     /locus_tag="VFB89_07565"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HZM27198.1"
                     /translation="MRALVKDSAGPGMTLRDVPQPVCGPSDVLIEVRHAGVCGTDLHI
                     WEWDSWASTRMRPPVIVGHEFAGRIAALGAEARAGGLLEEGDLVTAEGHIVCGHCLQC
                     RTGNAHLCQRTQVIGVDRDGAFAEYIVMPASNVMKLDGIPTEIGAIMDPLGNAVHTVL
                     EGEVAGSTVLVIGCGPIGCFAVGVARAAGASLVIASDYNPRRLDIARCMGAHVLLNPA
                     SDDVVARAHELTNGNGVDLVCEMSGHPAGHAQAFAAARLGGRVNLLGTPSRPTEVHFA
                     RDVIFKGLTLYGVTGRKMYGTWDQMRRFLRAGQLDPRPVLTHRFPLERIADAIQVIKE
                     GEAGKVILEIGA"
     gene            4780..5994
                     /locus_tag="VFB89_07570"
     CDS             4780..5994
                     /locus_tag="VFB89_07570"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HZM27199.1"
                     /translation="MTRTIDSSPLDSRLFQELEEFRRTGVYKRLNYLESPQGPVVRME
                     GRGDVIILSSNNYLGLSAQPEVVAAGKAALDQFGAGTSSVRFICGTYTVHRALEKACA
                     RLVGTEASLSFVSCWNANEAVPGTLLGENDLVLSDQLNHASIIDGLRLAKAITKCQTG
                     VYRHGDLTDLAEKLSAARDRRVRMVITDGVFSMEGSIAKLPDLVELCRKYDAVLVVDD
                     SHATGVLGDTGRGTAEHFGMVGEVDIITSTLGKALGGAAGGFAAGSAAVCDYLTQRAR
                     PQLFSNALPPTVAASALAAVEYLEAHPSRVKTLRDNARYFRDRLAALGFKPLPGETPI
                     VPVIVGETAFAIRLSDQLLDEGVFVTGFGFPVVPQGQARVRCQLSAAHTKDHLDQALS
                     AFQKVGRRLGLI"
     gene            6216..6905
                     /locus_tag="VFB89_07575"
     CDS             6216..6905
                     /locus_tag="VFB89_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27200.1"
                     /translation="MRMVTRSMAVVALALGIGAARLAAQAPVQGDFQWYVGGEAGVLV
                     FQTPAQTRGGMFSAGGHTLITARRTALLLSIDEGVGSDEQSAYFDATGTVQTVQFNDI
                     RKYSAVLTAFPIRSAAQPYLGVGVGILHTVNPQPTGTTFATPQALAQAQQDARDLGST
                     GFGTLIGGLQFKVGRMVAFGQYQLTSAQGVKTLESGASGRLLVGTTHSFSGGLRVSLG
                     GAREQPRVASK"
     gene            complement(6859..7506)
                     /locus_tag="VFB89_07580"
     CDS             complement(6859..7506)
                     /locus_tag="VFB89_07580"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HZM27201.1"
                     /translation="MKSPRRAAPLPVGFFERSAEIVARELVGTLIASTADGRWTLGRI
                     VETEAYLGYDDPASHGYRHRRHAQNAALYGPAASWYVYRSYGMHWCANLVCLGEGSGS
                     AVLLRAVEPLDGLDVMRHRRSVELVESLCSGPGRLCQALGITRSELDGRLMRGSPVEV
                     MAAADWSGVIEASPRIGISKAADWPLRFTAAGSRFVSRRTVTSKLRAVAREHPPG"
     gene            complement(7503..>8390)
                     /locus_tag="VFB89_07585"
     CDS             complement(7503..>8390)
                     /locus_tag="VFB89_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27202.1"
                     /translation="QRGVLQVNKMDNGQYEVAPAGAAPVAPEPSRNGPREAVEDGERR
                     GRSRGRGRGRGRERDRGAPGGRASPGDRAPVSRSEGTLTLAGAFQLISRALSELKPPV
                     AHEALRLRMVALRGREDALLQPERFIQLLRQANDAEVADVRKVSDTEYEVAPHRTESA
                     VSPPVASESSEVTAAPLTTEPAPEAGLANGQRPGLRFRRGSRGTVRVGEIPLIGVVPE
                     EPVAIEPQPEPLAPKTAPAKPARPRAARGRKQGTTPKVAEVSPSSADAAGLAEDAAAP
                     SGPPRRRRPSRPRARKPVE"
BASE COUNT         1450 a         2831 c         2607 g         1502 t
ORIGIN      
        1 cggcgtctac cgaaatcggc taaggattca catggcgctg caggccgacc caaccgttca
       61 gtatgccatt ctcctggcaa acggaatcag aaagccccga ctgtatcaaa aggactatct
      121 cacgccctca ccgtacaaca cctacctcca tcccggcttg ccgccaggac cggtgaattc
      181 gcccggacgt cgaagcatcg aagccgcact ctatccggca gacgtccatt atttgtactt
      241 cgtcgcgggc cctgatgggc gccatgtatt cagccggacc tacgatgagc acctgcgaaa
      301 cgtggggaaa atgcgaaggc tgatgaaaac gccgagcggc gaacagggag acaaaagcgt
      361 caacggtaaa cggtaagcgg tcaacgggag gttcgattgc accggagtcc cccgttcacc
      421 gtttaccgtt tacgcagttc tagtaccgtt caccgcttag gcagttctag taccgttcac
      481 cgtttacgct tttccagtat cgtcgcgcgg cgctttccac gtcgtcggcc cccaaaattc
      541 ccgagactac cgccacccca gctccacctg cactcaatac ggcggcaacg tcttccggcc
      601 tcaccccgcc gatcgcgatg catggtatgc cgttgcccgc cgcaaccagt ttttgaaaac
      661 cctcggcacc gagtgccaca cctgcatcgg gcttggtgcc gctggcgcgc cacggaccca
      721 ccccccagta gtcggccccg cgcgccgctt cggcctcctc gacggttccc gccgatgccc
      781 ccacgatgaa gcccgggggc gcgatacgac gcgccagcgg aagcgccagg tcatccgggc
      841 caaggtgaac gccggcagcc ccgctcgcga gcgccgcatc gatcctatcg tttaccagca
      901 ccggaatccg tagtacggcg acgagagtcc tcgtcgcgtc taccagctca cgcggggtcg
      961 cgtgcttgag ccgaacctga actgccgtca cgccgccctg ctctgctgcc cggccaacgt
     1021 cgagcaggtc tcgctcgcgc agtaacgcgt cgtcggtcac gagcatcagc cgcagaagcg
     1081 aagctagatt aggcgccatg ccagagatcc ctatgcctcg cattcacggc atccgcggcg
     1141 ctaccactgt cgaacgcaat gacgccgaag aaattctggg tgccaccgat gagctgctga
     1201 gagcgctcat ctcggccaac gatctccagc cggacgacat cgtcagtgcc tttttcactg
     1261 tgacgaacga tctggatgcc gcatttccgg ctcgcgcggc cgaggacttc ggctggaaca
     1321 tcgtggcctt gctccacgcg accgagatcc cggttcccgg gtcacttccc aaatgcatcc
     1381 ggctgcttgt ccacgcctat accacccgct cgcgcgggga aatcaaacat tgctatctgc
     1441 gccgggccat cagtctccga cccgaccgcg cgtgaccgac atccgcgtca cggcgcgggg
     1501 agccgagcgg tggaggcgag ggcatccctg gatccatcgg accgagctgc gcgatgagcc
     1561 cggccagccg gggctggtca acgtgcggga tccgcggggg aagttcatcg gccaggccct
     1621 ctactctccc agctctgaaa tccggctgcg gctgctcgag cccagtgacc aaccggtgga
     1681 tgcgaagtgg tggcggcacc aggtcgcgag cgcgctcgcg cgacgcaaaa gcatcgacgc
     1741 aacggcctac cgcgtagtgc acgccgaggc cgatggcctt ccttccctgg tcgtcgaccg
     1801 ctacgaccgg tgggtcgtcg ctcagctgct ctccggcgga ctggaaagcc aacgcgaaca
     1861 ggttatcgcg gcattgctcg atgtgctccg gcctgacggt ctgctgctgc ggcacgacgc
     1921 cccggctcgg cgtcgcgagc gcctgcctca ggaaacggtg cttgcctatg gaaaggtgcc
     1981 agaatggatc gaggtgcggg aggcgaatgt ccgatatctc gcggcgccct ggaccggcca
     2041 aaagaccgga gccttcctag accagcggcc caaccgcatt ctggctgcgg ccctcacacc
     2101 gcaaggcagc cgcacgctcg actgcttcgc ctaccacggg tcgtttgctc tccatctggc
     2161 gacccgatcg gccgaagtga ttgcgctgga ttccagcgcc gaagcactgg ttcgaggtgc
     2221 ggccaacgcg gagctgaacg gcttcaccaa cattgtctgg cgggaggcag atgcatttca
     2281 gacgcttcgc gccttcgagc ggaatcgcga tcggttcgat acgatagtgc tggacccacc
     2341 tgccttcgcc aagacccgtt cggccgtttc gcaggctatg cggggttacc acgagatcaa
     2401 tctgcgggcc atgcggcttc tcgcacccag cgggcacctg gtgacggcct cctgctcctt
     2461 ccacgtccac cgagccgagt tcctcgcgat gctggcttcc gctgctgccg atagcggtcg
     2521 gcgcctcgag cttcgggcgc tggtcggccc gggcgaggac catccggaga tcctcacgat
     2581 acccgagaca ggctatctca agggagccgt ggtccgcgcc ggtggcgaat ccgccctgaa
     2641 gcgttgacat gaaccgggcg tcttaacagc ttgcgagggc tgttatttca tccattccga
     2701 gcgcccgtct tgaaccaatc cataggcgca cccctgggcg cgccacgttt caaacgatgc
     2761 tgactgccgc tcaggccctc cgcctcgatc ggcgtcgacg tcctcgcccg tcgaccaagc
     2821 ggcaatacga cgagtacatc ctgcaaaaga tcgaggccta caagaactcg ctatcccggg
     2881 atcagttgct gctgcttggc gacgaggcca atcaagagct tctcgctgct accgaaggcc
     2941 agttcgtgct gacggaaatc ctgatgttgg acagcgtgga cgacttgatc aagcgccgcc
     3001 tcaagctcaa gccattcaag aagtggaaag cctggatcaa ggatctgcga gatgcgcagc
     3061 gggagccgac ccattggggc ctcgagccgg aatgcccggt cgcgggtcta gtgcgacggg
     3121 tggagccgca agacgaggcc gtcgtggttg gcggtgccgc ggacggcgcc gcgttcctgc
     3181 tcgcggcgca cgaaattccg gtcaccttcc tggccgccga tctcggcagc gtcgatcgtg
     3241 tcgaatcccg ggtggaagat gaatcgctgg ctgtgccagt catcgcgacg gtggtggact
     3301 tcgagctctg gtgcccgcct ctgcccgaca gcatcgccat caccgttgtg gatgccggag
     3361 aggtgggcga gcttgacccg gtagttcggg ctgggctgat caacgctctg caaagccgga
     3421 cctgtgaggc cggtgttcat gtgctgctcc ctggcagagg cgggatggca ccagaagcgt
     3481 tgcttcacca ttacgatggg tggatccgcg aggcagacgg aaggcccaag cgccgaggcg
     3541 gccggtcggc gggcatcgtc ctcagctggg cccccgccaa accttctgcg ctccctgccg
     3601 acgacgcggc ggcctgctga cgcctcccta caggccgccg cacgcactac acttcaaccc
     3661 gacatgaaaa cgcttccgat cgagcgcaca gcggaggact accttcccgc cctcccctga
     3721 cccgcgctca ttgcctgccc ccatgcgcgc cctcgtcaaa gactccgcag gtcccggcat
     3781 gacgttgcgg gacgtgccgc agccggtgtg cggaccgagt gacgtactga tcgaagtccg
     3841 ccacgccggc gtctgtggga ccgatctcca catctgggaa tgggacagct gggccagcac
     3901 ccgaatgcgt ccgccggtca tcgtcgggca tgagtttgcg ggccggattg cagcgctggg
     3961 tgccgaagcg cgcgccggcg gcctgctcga ggaaggcgat ctcgtgaccg cggaaggaca
     4021 catcgtgtgc ggccactgcc ttcagtgccg cactggaaac gctcacctct gccaacggac
     4081 tcaagtcatc ggggtcgatc gagacggggc gtttgccgag tacatcgtga tgccggcatc
     4141 gaacgtcatg aagctggacg gaattcccac cgagatcggc gccattatgg accctctcgg
     4201 caacgcggtt cataccgttc tcgaggggga ggtcgccggc agcacggtgt tggtgattgg
     4261 atgcggcccc atcggctgtt ttgctgttgg tgtagcccgt gccgcgggcg cctccttggt
     4321 gattgccagc gattacaacc cccgtcgtct cgacatcgcc cgctgtatgg gcgcccatgt
     4381 tctactcaat cccgctagtg acgatgtcgt tgcccgggcc cacgagctta ccaacgggaa
     4441 cggtgtggat ctcgtctgtg aaatgagcgg ccatcctgcg gggcacgcgc aggcgttcgc
     4501 cgccgctcgc ctcggggggc gggtaaacct cctggggacg cccagccgcc ccacggaggt
     4561 tcattttgcg cgggacgtca tcttcaaggg gctcaccctt tacggggtga ccggccggaa
     4621 gatgtacggg acttgggatc agatgcgccg gtttcttcgc gccgggcagc tcgatcctcg
     4681 ccccgtgctg acgcaccgct ttccactgga gcggatcgcc gacgccattc aagtgatcaa
     4741 agagggagaa gccggtaagg tgatcctgga gatcggcgcg tgacccgcac catcgactcg
     4801 agcccgctcg actcgcggct tttccaagag ctggaagaat tccgtcgcac cggggtctat
     4861 aagcggctca actacctcga aagtccccaa gggccggtcg ttcgcatgga aggacgcggg
     4921 gacgtcatca ttctgtcctc caacaactat ctcggtctga gcgcccagcc cgaggtggtc
     4981 gccgcgggca aagccgcgct ggaccagttc ggtgccggca catcctccgt acgctttatc
     5041 tgcggtacct acacagttca ccgtgcgctg gaaaaggcct gtgctcggct ggtggggacg
     5101 gaagcgtcgc tcagcttcgt gagctgctgg aatgccaacg aagcagtccc cggaactctc
     5161 ctcggagaga atgatctggt tctcagcgac cagctcaacc atgcctcgat cattgatggg
     5221 ttacgcctgg ccaaagcgat tacgaagtgc cagaccggcg tgtaccgcca cggggatctg
     5281 accgatttgg ccgagaagtt gagcgccgcg cgggatcgcc gtgttcgcat ggtcattacc
     5341 gacggggtgt tttcgatgga aggctcgatc gcaaagctgc ccgatctggt cgagctgtgc
     5401 cggaagtatg acgcagtcct cgtggttgac gactctcatg cgaccggtgt actcggggat
     5461 accgggcgcg gcaccgcgga acatttcgga atggtcggtg aagtcgacat catcacctcc
     5521 acacttggta aggccttggg tggcgcagcc ggtgggttcg cggccggctc cgccgctgtg
     5581 tgtgattacc tcactcagcg ggcccggcct caactcttct ccaatgcgtt accgccaacc
     5641 gttgccgcca gcgcgctcgc ggccgtggaa tatctcgaag ctcatccgtc gcgcgtgaag
     5701 accctgcgag acaatgcccg atattttcgg gaccggctcg cagcgcttgg attcaaaccc
     5761 ctccccggcg aaacccctat cgtaccggtc atcgtgggtg agaccgcctt cgccattcgc
     5821 ctcagcgatc aattgctgga tgaaggcgtg ttcgtcaccg gcttcggctt cccggtcgtg
     5881 ccgcaaggtc aggcacgggt ccgttgccag ctgtccgcag cccacacgaa agaccatctc
     5941 gaccaggcgc tctccgcctt ccagaaagtg ggtcggcggc tcggcctgat ctgatcagtt
     6001 cgagataggc ccactccttg caccctcggg ggtgcaagtt tggtcgccag gccgcggcgt
     6061 cgatcggcgg tcactttcgc aagcgcttac acaacaacaa cttggcgagg gcagtcggga
     6121 gcggagaacc cctccctccg gatcgcactc caccggaact cacgcaattt tccggtccgc
     6181 acgcagtcgg tcgtctgacc gagggaggaa ctctgatgag gatggtcact cgcagcatgg
     6241 ccgtcgtggc gctggcgctc ggaatcggcg cggcacgtct ggccgctcaa gcgccggtcc
     6301 agggcgattt ccagtggtac gtcggcggtg aggccggtgt cctcgtgttc caaaccccgg
     6361 cccagacaag aggcggaatg ttttcagccg gtgggcacac gctcatcacg gcccgacgca
     6421 ccgcgctcct gctctcgatc gatgagggcg tcggatctga cgagcagagc gcctacttcg
     6481 atgcgaccgg taccgtgcag acggtccagt tcaacgacat ccgcaagtac tccgcggtgc
     6541 tcacggcatt ccccattcga tccgccgcac agccgtacct cggcgtgggc gtcggtattc
     6601 tccataccgt aaaccctcag ccgaccggca ctacgttcgc cacgccgcag gctctcgctc
     6661 aggcgcagca agacgctcgc gacctcggca gcacaggctt cggcacgctg atcggtggat
     6721 tgcagttcaa ggtgggtcgg atggttgcct tcgggcagta tcagctcacc tcggcgcaag
     6781 gcgtcaagac actggaaagc ggcgccagcg gccggctgct ggttggcacg acccactcct
     6841 tcagcggcgg tttgcgggtc agcctggggg gtgctcgcga gcaaccgcgc gtagcttcga
     6901 agtaacggtc cggcgcgata caaaacgcga ccccgcggcg gtgaagcgaa gcggccagtc
     6961 ggccgccttc gatatcccga tccgcgggct cgcttctatt actccgctcc agtccgccgc
     7021 ggccatgact tcgaccggcg accctctcat cagccttcca tcgagctcgc ttcgagtaat
     7081 gccgagcgcc tgacacaggc ggccggggcc cgagcacagc gattcgacca gctcaacgct
     7141 tcgacgatgc cgcatgacgt cgagcccatc cagcggctcg acggcgcgca gcagcaccgc
     7201 gctccccgag ccctccccca ggcacaccag gttcgcgcac caatgcatgc cgtatgaccg
     7261 gtaaacgtac cagctcgctg ccggcccata gagcgccgca ttctgcgcat ggcgccgatg
     7321 acgatagccg tgcgatgccg gatcgtcgta cccgagatac gcctcggtct caacgattcg
     7381 gcccagcgtc caacgtccat cggcagtgga tgcgatcagc gtaccgacca gctcgcgagc
     7441 gacgatctcg gccgaccgct caaaaaaacc gacgggcagc ggggctgccc gtcgcggact
     7501 tttcattcca ccggctttcg agcacgaggt cgcgatgggc gccgtcggcg tggtggcccg
     7561 ctcggcgccg cggcgtcctc cgcgaggccg gcagcgtcgg cagaggatgg cgaaacctcg
     7621 gccaccttcg gggtagtccc ctgcttccgt ccgcgtgcgg cgcggggacg cgccggcttg
     7681 gcaggcgcgg tcttcggcgc cagaggctcg ggctggggtt ctattgcgac gggttcctcc
     7741 ggtacaacgc cgatcagcgg tatctcgccc actcgcacgg taccccgcga tcctctccgg
     7801 aacctgaggc ctggccgttg cccattggcg aggccggcct cgggtgccgg ctcggttgtg
     7861 agcggcgcag cggtgacctc ggaggactca ctcgcgactg ggggtgagac tgcactttcg
     7921 gttcgatgcg gcgcgacttc gtattccgtg tcgctcactt tgcggacgtc ggcgacctcg
     7981 gcgtcgttgg cctggcggag cagctgaatg aaccgctccg gctgaagcag agcgtcctct
     8041 cggcctcgca gtgcgaccat ccggaggcgc agggcctcat gcgccacggg aggctttagc
     8101 tccgacaacg cccgactgat cagctgaaat gcgccggcca gggttagtgt cccttcgctt
     8161 cggctaaccg gagcgcgatc gcccggggat gcacggcctc ccggcgcccc gcggtcccgc
     8221 tctcgccccc gccctcgtcc gcgaccacgg gagcggccgc gtcgctcacc atcctcgacc
     8281 gcttcccgcg ggccgttccg cgacggctct ggtgccaccg gcgcagcacc ggcgggggcc
     8341 acctcatact ggccgttgtc catcttgttc acctgcagaa ccccgcgctg
//
LOCUS       DASRJQ010000342         2681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_419502, whole genome shotgun sequence.
ACCESSION   DASRJQ010000342 DASRJQ010000000
VERSION     DASRJQ010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419502"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2242)
                     /locus_tag="VFB89_07590"
     CDS             complement(<1..2242)
                     /locus_tag="VFB89_07590"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HZM27203.1"
                     /translation="MSRGFQTTRLLGLVAVCWCAALPLQAQSNADRFLTDEYTRSHDF
                     DLVHQRIEVGAFSWDSLSFDGKVSTVLVARKPALADIVLDAGRRLEIRSVTQDKTTLK
                     FDRSYDSLVIHLSRPAEFDDTLKFVIQYHGKVAQGQGLYFFDAESGRWGRQVYSGGGT
                     DGNPNWFPTYGGAEDKLTWELVATVPRDLTVVSNGRLVKDRKGPNNTHTVEWAQEQPA
                     SSYLVSLVAAPLVKLSDKWRKVPLAYYVAPADSARARVVFSMTPRVLEVYEQLLGVPF
                     PWAKYAQVTVADYFGGMENVSATTLADWIPDRLALLDAPWYIRTLIPHEVAHNWFGNY
                     VTTANWASYWLNEGFAQFMVGQYWRVAVSEDASEEAYLTDYSEYADDDARRRMPLAAL
                     GSNNIYPKGSLVLRMLRLELGDRRFWAGVQRYLMRHAFANATSADLQRAFLEATGDNL
                     SWFFDQWVYRAGHPEFSIGAAYDSTARRLTLTVQQTQQDTLHADSTGLRYSVPAAFRG
                     QVAIRVGTSAGDVVRAYQIRQRAETLIVADLPGPPTMVVFDDENRMLKSLRFDQPTSW
                     LATQARKAPNVWNRAWAIDQLAARTAEGHAGVALVDAAKSGSDVTVRSLAAQGLGRFR
                     AELALPGLKAALGDTSAVVREAAVRTLGSIRGPDALGLARAAFSSDSSYAVRAAAVTT
                     IAQLDSAQAHDVIVQALGMPSYRSMVQTAALAAALHTEDASLVPTLEERLGDQPLVAI
                     ALAWFAT"
     gene            complement(2239..>2681)
                     /locus_tag="VFB89_07595"
     CDS             complement(2239..>2681)
                     /locus_tag="VFB89_07595"
                     /inference="COORDINATES: protein motif:HMM:NF013514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HZM27204.1"
                     /translation="DRFNIRVASAIAMRRAVDRCLRSFAARETLGLAGHGQRLPGCER
                     MQAPPQYWILIDGLPLPEIGMEHRALVDGDALCHSVAAASVVAKTVRDALMRRLAVRY
                     PGYGWDTNVGYATDGHQEGLRLLGPTRHHRRSFFPIAQLQLDLS"
BASE COUNT          493 a          861 c          885 g          442 t
ORIGIN      
        1 gggtcgcgaa ccaggcgagc gcgatggcca cgagcggctg atcccccagc cgttcctcca
       61 gcgtggggac cagcgaggca tcctcggtgt gcagtgccgc ggccagggcc gccgtctgaa
      121 ccatcgaacg gtatgacggc attccgagtg cctgcacgat cacgtcatgc gcctgcgcgg
      181 aatcgagctg agcgatcgtg gtcacggccg cagcgcggac tgcatagctc gagtcggagg
      241 aaaatgcagc gcgggccagg ccgagggcat ccgggccgcg aatcgagcca agggttcgga
      301 cggcggcctc gcggaccaca gccgacgtat ccccgagagc ggccttgaga cccggcaacg
      361 ccagctccgc ccgaaaacga cccaggccct gcgcggccag actgcgaacg gtcacgtcgc
      421 tcccactctt tgctgcgtcg acgagagcga cgccggcgtg cccctctgcc gtcctcgcag
      481 ccagttgatc gatcgcccag gcacggttcc atacgttcgg ggccttccgg gcctgggtag
      541 caagccagct ggtgggttgg tcgaaccgaa gcgacttgag catccggttc tcgtcgtcga
      601 acaccaccat ggtcggcggc ccgggaagat cggccacaat caacgtctcg gctcgctgac
      661 gaatctgata ggcccgaacc acgtcaccgg ctgaggtgcc gacccgaatc gcaacctgac
      721 ctcgaaacgc tgcggggacg ctgtaacgca ggccggtgga gtcggcgtgc agggtgtctt
      781 gctgagtctg ctggacagtc agggtgagac ggcgggccgt gctgtcgtag gcggcaccaa
      841 tggaaaactc cgggtggccg gcgcgataca cccactggtc gaagaaccag gacaggttgt
      901 cgccggtggc ctctagaaat gcccgctgca gatcggcgct ggtggcgttg gcaaaggcat
      961 gccgcatgag ataacgctga acgccggccc aaaaacggcg atcgccaagc tccagtcgaa
     1021 gcatgcggag caccagcgag cctttgggat agatgttgtt cgatcccagc gctgccagcg
     1081 gcatccggcg ccgggcgtcg tcgtccgcat actccgagta atcggtgagg taggcttcct
     1141 ccgaggcgtc ctcgctcacg gccactcgcc agtactgccc caccatgaac tgggcgaatc
     1201 cctcgttgag ccagtagctg gcccagttag ccgtggtcac atagttgccg aaccagttgt
     1261 gggccacctc gtgcggaatg agggtcctga tgtaccaggg cgcgtcgagc agagctaggc
     1321 gatcgggaat ccaatcggcc agcgtcgtcg cgctcacgtt ttccatgccg ccgaagtagt
     1381 ccgcgaccgt cacctgggca tatttggccc agggaaacgg aacgccgagc agctgctcgt
     1441 agacctcgag cactcgcgga gtcatcgaga acaccacccg tgcgcgggcg ctgtcggccg
     1501 gggccacgta ataggcgagc gggactttgc gccacttgtc gctcagcttg accagcggcg
     1561 ccgccaccag cgagaccaaa taactggaag ccggttgttc ctgagcccat tccacggtgt
     1621 gggtgttgtt gggccccttg cgatctttca ccagccggcc gttggacacc accgtgagat
     1681 cgcgcggaac ggtcgcgacc aattcccagg tcagcttgtc ttccgcacca ccgtaggtgg
     1741 ggaaccagtt gggattcccg tcggtcccgc cgccgctgta cacttgccgt ccccagcggc
     1801 ccgattctgc atcgaagaag tagagtccct gcccctgcgc gacctttccg tgatactgga
     1861 tcacgaactt gagcgtgtcg tcgaattctg ccgggcggga caggtggatg accagcgagt
     1921 catatgaacg atcgaacttg agggtggtct tgtcctgagt caccgagcgg atctcgagac
     1981 ggcgaccggc gtcgagcacg atatccgcaa gcgcaggctt tcgggccacg agtacggtgc
     2041 tgactttgcc gtcgaacgac agcgaatccc aggagaacgc gcctacttcg atgcgctggt
     2101 gcaccagatc gaagtcgtgc gaccgggtgt attcgtccgt gaggaaccga tcggcattgc
     2161 tctgggcctg gagcggaagt gccgcgcacc agcacaccgc cacgagtccg agcagccgag
     2221 tggtttggaa gccgcgactc acgacagatc gagctggagc tgagcgatgg ggaagaagct
     2281 gcgtcgatga tgccgggtcg gcccgagcag gcggaggcct tcctggtggc catcggtggc
     2341 gtagccgacg ttggtatccc agccatagcc cgggtaccgc acggcgaggc gccgcatcag
     2401 cgcatcgcgc acggtcttgg ctacgacact cgccgcggcg accgagtggc acagggcgtc
     2461 gccatcgacc agcgcgcggt gctccatgcc gatctcaggg agtggcaggc catcgatcag
     2521 gatccagtat tgcggcggtg cctgcattcg ttcgcagccc gggagacgct ggccatgccc
     2581 cgccaacccc agcgtctccc gggctgcgaa cgagcgcagg cacctatcga ccgcgcgccg
     2641 catcgcaatg gcggaggcca cccggatgtt gaaccggtcg a
//
LOCUS       DASRJQ010000343         2493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2329076, whole genome shotgun sequence.
ACCESSION   DASRJQ010000343 DASRJQ010000000
VERSION     DASRJQ010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2329076"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            272..1555
                     /locus_tag="VFB89_07600"
     CDS             272..1555
                     /locus_tag="VFB89_07600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZM27205.1"
                     /translation="MLASGGLISKIASLDRPRICFIVEAVYPDQIGGIEIQTYAIAAG
                     LAARGLPVTLVARRIRPESPAREQTGNLRVVRLGARGRLKGTGWRAVAPLLGFIASVL
                     AWLVRNMGRYDAVLAQGVKVLFLPASTITWLRLRPCIVKIDSPIELWEEVSAESLRRM
                     GLSRSAAPVRMWRAVRAALLRRVGDFVVISAEIRDALLALGVESGRIRFIPNGTDVER
                     FHPVSLEQRLTLRRKLGLPEGPVLLNFTGRLSAAKGLMLLAGIWKSLVTEGRSVHLVL
                     VGTGGTSFDNCEAELRAYVEQHGLGPTVTFTGEVDNVPEYLQASDLFVFPSEYEGFPL
                     SLFEAMACGLPVASTRVGSAREILIEGEHGALAEPKNAEQMRSAIVWLLDHQGQWPAI
                     AERVRRLVVEQFSLSAVIDQYAALFREVVARDRSS"
     gene            complement(1572..>2493)
                     /locus_tag="VFB89_07605"
     CDS             complement(1572..>2493)
                     /locus_tag="VFB89_07605"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZM27206.1"
                     /translation="VRALVRQNSFHAGRLKDLDVQVVEGDLTEAAALQAAMRGIETVY
                     HAAAATHNGWSENESTTIEGTRLLLDAALGQNVGRLVLLSSLAVFDTRKSRGRVIRED
                     SPLLPNPRQNGAYAYSKIVVEGLALASHREHGLGVTVVRPGMVIGPLGHVWFPHLGYH
                     YGDRLFLTFGRGDQVLPLTYVENTVDGIYRAATHERAIGQAYNLVDEGAVTARQYIEQ
                     LISTTRAPGRIIGLPYPLPYCATATYELAAGLNLMPKGVTSRAQLKAKWYPPRFDSSK
                     ARNELGWTARVPLGVGLQETFSWYARRNGR"
BASE COUNT          418 a          774 c          805 g          496 t
ORIGIN      
        1 gtaaggcaca aaccgatcca aagcgcattg ggggctggat cgcgcagctg cacaggttgg
       61 cccgagtcgc ggcacgaagc tggtagcgct ccaccggaag tgccgggccg ctcgcgcatc
      121 gtcggcagac tggcttacaa ccatggccga cgcccatgcc gaccagctaa gccatcagag
      181 tcgcagaagt ccatcgctgt ctggcacgac ggccaatgtg aagacgggcc acccctggcc
      241 ttcccgcaga tccctgagca cggctgcgga tatgctcgcg agcggaggac tcatcagcaa
      301 gatcgcttcc ttggaccgtc ctcgcatctg ctttatcgtc gaggcggtct acccggatca
      361 gatcggcggg atcgagatcc agacctatgc tatcgccgca gggctcgccg ctcgcggcct
      421 cccggtcacc cttgttgccc gacgcatccg cccagagtcg cctgcccgcg agcagaccgg
      481 caacctacgg gttgtgcgct tgggcgcgcg aggaaggctg aagggcacgg gctggcgcgc
      541 ggttgccccc ctgctggggt ttatcgcatc ggttctggcc tggctggtcc gcaacatggg
      601 ccgttacgac gcggtgttgg cccaaggggt gaaggttctt ttcctgcctg ccagcacgat
      661 tacctggctt cgactccggc cctgcatcgt caagatcgac tctcctatcg agctttggga
      721 ggaggtctcc gcggagagcc ttcgccggat gggcctgtca cggtcggcgg cgccggttcg
      781 catgtggagg gcggtacggg ccgcgctgct gcggcgcgta ggcgactttg tagtgatctc
      841 agcggagatc cgcgatgccc tcctcgccct gggtgtcgag tccggacgca ttcgattcat
      901 ccccaacgga accgacgtgg agcgtttcca tcccgtctcg ctcgagcaac ggttgacgct
      961 ccggcggaag cttggcctgc ccgaagggcc cgtcctgctc aattttacgg gccgcctctc
     1021 tgcggcgaag ggccttatgc tgctcgccgg tatctggaag agcctcgtta cagaagggcg
     1081 ctccgtgcac ctggtgctgg tgggtaccgg cggaacctcg tttgacaatt gtgaggcgga
     1141 gctacgggcc tacgtggagc aacatgggct cggaccgacc gtcaccttca ccggcgaggt
     1201 cgacaatgtc ccggagtacc tgcaggcttc ggacttgttc gttttcccga gcgagtatga
     1261 gggctttccg ctttcacttt tcgaggcaat ggcttgcggc ctgccggtcg cgtcgacccg
     1321 cgtcggatct gcccgggaga ttctaatcga gggggagcac ggtgcgctgg ctgagccgaa
     1381 gaacgctgag cagatgcgta gcgcgatcgt gtggcttcta gatcatcagg ggcagtggcc
     1441 tgccattgcg gagcgggtgc gacgactggt agtcgaacag ttcagtctca gtgcggtgat
     1501 cgatcagtac gcggcattgt tccgggaagt agtcgcgcgc gacaggtcgt cgtgattttg
     1561 gcgccgcctg ctcagcggcc gttccgcctc gcgtaccagg agaatgtttc ctgcagacct
     1621 accccaagtg gaacgcgggc tgtccaacca agctcgtttc gtgccttact gctgtcgaag
     1681 cgggggggat accattttgc tttgagctga gcccgcgaag tgactccctt gggcatcagg
     1741 ttcaggcccg cagctaactc gtaggtcgcg gtagcgcaat acggcagagg atatggaagg
     1801 ccgattatcc gacccggggc gcgggtagtg ctgatcagct gctcgatgta ttgccgggcc
     1861 gtgacggcgc cctcatctac cagattgtac gcttggccaa tcgcgcgctc gtgggtggct
     1921 gcccgataga tcccgtctac agtgttctcg acgtaggtaa gtggcagcac ttgatccccg
     1981 cggccaaagg tgaggaacag ccggtccccg tagtggtatc ccaggtgcgg gaaccagaca
     2041 tggccgagcg gaccgatgac cattcccggc cgtactaccg tcacgccaag cccatgctcg
     2101 cgatgactcg cgagcgccag cccctccacc acgatctttg agtaggcata agctccattc
     2161 tgccttggat tgggcaggag cggagagtcc tctcgaatga ctctgccgcg gctcttccgg
     2221 gtatcgaaca ccgccaacga gctcaaaagc accaggcgtc cgacattctg gcccagtgcg
     2281 gcatcgagta gcaatcgggt gccttctatg gtcgtagact cgttctcgct ccagccattg
     2341 tgggtggcag cggcggcgtg gtataccgtt tcgatccccc gcatggcggc ctgaagtgcg
     2401 gcggcctcgg tcaggtctcc ctccacgacc tggacatcca gatccttgag tcggcccgcg
     2461 tggaagctgt tctgccggac cagcgcgcgg act
//
LOCUS       DASRJQ010000344         2809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2592683, whole genome shotgun sequence.
ACCESSION   DASRJQ010000344 DASRJQ010000000
VERSION     DASRJQ010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2592683"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /locus_tag="VFB89_07610"
     CDS             <1..1249
                     /locus_tag="VFB89_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HZM27207.1"
                     /translation="EVEEAVDPNAPPELDETDSGIVKLCNQIIIDAYNRGASDIHVEP
                     YGKTAPTQIRLRIDGDCTKYLEVPSAHRNAVVQRFKIMSKLDIAEKRKPQDGKIRFKG
                     PMGTIELRVATIPTSGGNEDVVMRLLAASKPLPLERMGFSDRNITEFKKILEKPYGIC
                     LVVGPTGSGKTTTLHSGLGYINTVDMKIWTAEDPVEITQAGLRQVQVHPKIDFTFAVA
                     MRAFLRADPDVIMVGEMRDHETAATGIEASLTGHLVFSTLHTNSAPETITRLLDMEID
                     PFNFADALLGIMAQRLVRTLCKDCKEEYAPTQEEFAELMEEYGLEHWDKLGIRFSPDF
                     KLFRPKGCPKCGNTGYKGRMGIHELLVATDEIKRLIQKRTPIEELRKQALLDGMTTLL
                     QDGIQKVVKGVTDFKQVRAVCIK"
     gene            complement(1358..2608)
                     /locus_tag="VFB89_07615"
     CDS             complement(1358..2608)
                     /locus_tag="VFB89_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27208.1"
                     /translation="MRPTLLLFVALLSLGRAAWPQGNPVGPEFRVNTYTTNAQTGSAV
                     ASDGSGNFVVVWESNGQDGSISGVFGQRYASAGPPLGPEFRINTYTTDNQLYPSIAAD
                     ATGNFLVVWSGYPDGSSFGVFGQRYTNSGTPLGVEYRVNAFTTAGQSHPAVASATSGI
                     FVIVWDSQVQDGSIIGIFGQRFAGTGAPLGPEFRINTYTTGPQGQPRVAADAAGNFVV
                     VWVSTPQDGSDSGVFGQRYASSGAPLGPEFRANSFTTDNQFAPNVASDASGNFVVVWT
                     SYAQDGSSSGVFGQRYASSGAPLGAEFRINTFTSGEQLRASVAADAGGNFVVTWQGYG
                     QDGSNWGIVAQRYSSSGVPLGGEFLVNTLTTGDQLQPSVSSDAAGHFVVAWQSYAQDG
                     SDNGIFAQRYSMIVPVELQSFRVE"
BASE COUNT          547 a          905 c          926 g          431 t
ORIGIN      
        1 ggaggtcgag gaggcggtgg accccaacgc ccctccggag ctggacgaga ccgacagcgg
       61 gatcgtgaag ctctgcaacc agatcatcat cgacgcctac aaccgcggcg cctcggacat
      121 ccacgtcgag ccctacggca agaccgcgcc cacccagatc cggctgcgca tcgacggcga
      181 ctgcaccaag tacctggagg tgccgtccgc gcaccgcaac gcggtggtcc agcgcttcaa
      241 gatcatgtcc aagctggaca tcgcggagaa gcgcaagccg caggacggca agatccgctt
      301 caagggcccc atggggacca tcgagctgcg cgtggccacc atccccacct cgggcggcaa
      361 cgaggacgtg gtgatgcgcc tgctggccgc cagcaagccg ctgccgctgg agcggatggg
      421 cttctccgac cggaacatca ccgagttcaa gaagatcctc gaaaagccct acggcatctg
      481 cctggtggtg gggcccacgg gctccggaaa gaccacgacc ctgcactcgg gcctgggcta
      541 catcaacacc gtggacatga agatctggac cgcggaggac cccgtcgaga tcacgcaggc
      601 gggcctgcgc caggtgcagg tccacccgaa gatcgacttc acgttcgcgg tggccatgcg
      661 cgccttcctg cgcgccgacc ccgacgtgat catggtcggc gagatgcgcg accacgagac
      721 ggcggccacg ggcatcgagg cctcgctcac cggccacctg gtcttctcga cgctccacac
      781 gaattccgcc cccgagacca tcacccgcct gctggacatg gagatcgacc ccttcaactt
      841 cgccgatgcg ctgctgggga tcatggccca gcgcctggtg cggacgctct gcaaggactg
      901 caaggaggag tacgcgccca cccaggagga gttcgcggag ctcatggagg agtacgggct
      961 agagcactgg gacaagctgg gcatccgctt ttccccggac ttcaagctct tccggcccaa
     1021 gggctgcccc aagtgcggga acaccggcta caagggccgc atgggcatcc acgagctgct
     1081 ggtggccacc gacgagatca agcggctcat ccagaagcgc acgcccatcg aggagctgcg
     1141 caagcaggcg ctgctcgacg ggatgaccac cctgctccag gatggcatcc agaaggtcgt
     1201 caagggcgtg acggacttca agcaggtccg cgcggtctgc atcaagtaag cgctcggccg
     1261 gcgcaaggct caggccctcg ggcgcgaggc gtcgggctgt cgggcgcgag ggtgcacgcc
     1321 ccttcgcgcg cctgaaggaa tcgggcggac tacgccccta ctccacgcgg aagctctgca
     1381 gttcgacggg aacgatcatg gagtagcgct gggcgaagat gccgttatcg gatccgtcct
     1441 gcgcgtagct ctgccaggca acgacgaagt gacccgcggc gtcggagctg acggagggct
     1501 gaagttggtc gccggtcgtg agggtgttga cgaggaactc gccgccgagc ggcacgcctg
     1561 agctgctgta gcgttgggcg acgatgcccc agttcgagcc gtcctgccca tagccttgcc
     1621 atgtcacgac aaaattgccg cctgcgtcag cggcgacgct ggcacgaagc tgttcgcccg
     1681 aagtaaatgt gttgatgcga aactccgcgc cgagcggcgc gcctgagctt gcgtaacgct
     1741 ggccgaagac accggaactc gagccgtcct gcgcatagct ggtccagacg acgacgaagt
     1801 tgccggaggc gtcgctagcc acgttcggag cgaactgatt gtcggtcgta aagctgttgg
     1861 cacggaactc cggtcccagc ggtgcgcccg agctggcata gcgctggccg aagacacccg
     1921 agtccgaacc gtcctgcggg gtgctgaccc atacgacgac aaaattgccg gcggcgtcgg
     1981 cggcgacacg tggttgcccc tgtgggcctg tggtgtaggt gttgatacga aactccggac
     2041 ccagcggcgc ccccgtgccg gcgaagcgct ggccgaatat tccgatgatc gagccgtcct
     2101 gaacctgact atcccagacg atgacgaaga tgccagacgt ggccgaggcc acggcagggt
     2161 gactctggcc ggccgtcgtg aacgcgttga cgcggtactc cacgccgagc ggcgtgccgg
     2221 agttggtgta gcgctgaccg aagacgccga agctggagcc gtcagggtaa ccgctccata
     2281 cgaccaggaa attgcccgtg gcgtcggctg ctatggatgg atagagctga ttatcggtcg
     2341 tgtacgtgtt gatccggaac tccggaccga gcggcgggcc ggcactggca tagcgctggc
     2401 cgaaaacgcc ggagatcgag ccgtcctgtc cgttgctttc ccagacgacc acgaagttgc
     2461 cggagccgtc ggatgccacg gcagatcccg tctgagcatt cgtcgtgtag gtgttgacgc
     2521 ggaactcagg accgacgggg tttccctggg gccacgcggc acgtccgagg gaaaggaggg
     2581 cgacgaagag aaggagcgtc gggcgcattt tgacacctcc ccctgaaacg ctgatcgagg
     2641 tcagagcaga caccctacag ttctcgatca cccggagcaa ggcgtcgtcg catccgtctc
     2701 aagacggata actcgcaagg ataatcgtta cgagactacc cgcccgggaa cgaatctgct
     2761 ccgccccgac ctgcaccatg gagtgcgacg gtcggcgtca catcctgta
//
LOCUS       DASRJQ010000345         4277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_4204629, whole genome shotgun sequence.
ACCESSION   DASRJQ010000345 DASRJQ010000000
VERSION     DASRJQ010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4204629"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            356..1555
                     /locus_tag="VFB89_07620"
     CDS             356..1555
                     /locus_tag="VFB89_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZM27209.1"
                     /translation="MARLAQTPGLTEVQRDILSTVRAFAEKEIIPHAQRLEHADEYPS
                     DIVDGMREMGLFGLTIEEEYGGLGESLLTYVLVVEELSRGWMSISGIVNTHFIVAYLI
                     SNHGSLEQKARLLPKMATGEVRGAFSMSEPGVGSDVAGIQSRAVRDGDTFVLNGQKMW
                     LTNGGYSSVVATLVKTDLDAPTVYGNMTTFLLEKEPGFGETAPGLSIPGKLEKMGYKG
                     VETTEMILDGHVVPASAVLGGVDAGLGRGFYQMMDGIEVGRVNVAARACGIAIRAFEL
                     AVAYAQQRSTFGKKIAEHQAVAFKLAEMATKIEAAHALMVNAARLKDAGQRNDVEAGM
                     AKLLASEYCAEVVQESFRIHGGYGYSKEYEIERLMREAPFLLIGEGTSEIQKTIISRG
                     LLKEYKL"
     gene            complement(1601..2008)
                     /locus_tag="VFB89_07625"
     CDS             complement(1601..2008)
                     /locus_tag="VFB89_07625"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HZM27210.1"
                     /translation="MRTPRQILEESTNIAVVGASRHEEKAAYAVPLQLKLHGWRMIPV
                     NPYADRIWGEVCYPRLIDIPEPVDLVNVFRPSGQAAAVVRDAAAIGAKAVWLQQGIVS
                     AEGREIAAEAGMDYVEDLCTAVIRAIERLTKRV"
     gene            complement(2064..3089)
                     /locus_tag="VFB89_07630"
     CDS             complement(2064..3089)
                     /locus_tag="VFB89_07630"
                     /inference="COORDINATES: protein motif:HMM:NF038135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rv2578c family radical SAM protein"
                     /protein_id="HZM27211.1"
                     /translation="MRWNNLSVAAPESPGALPLAIPGSVARTFDSPGFAGMTFYEIQA
                     KSIISRVPSASRMPFEWTINPYRGCSHACVYCFARNTHTYLDLDAGHDFDTKVVVKVN
                     APQLLRKELAAAKWTGAHIAMGTNVDCYQRAEGKYGLMRGIIGALRDFANPFSILTKG
                     TLITRDLDLLRQAAEVTRVGVSFSVGFVDEETWRSVEPGTPSPLRRLEAVRRFADASF
                     PVHVLMAPILPGLTDTDESIEATVAAIAASGAAGVSPLILHLRPGAREWYAAWLGRTH
                     PDLVDRYRSIYGKGSYAPKAYQREVSARVAIAARRHGIGRAHMSQHRGIEEAPAAPPA
                     AEQLTLL"
     gene            complement(3246..3644)
                     /gene="trxA"
                     /locus_tag="VFB89_07635"
     CDS             complement(3246..3644)
                     /gene="trxA"
                     /locus_tag="VFB89_07635"
                     /inference="COORDINATES: protein motif:HMM:NF025946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HZM27212.1"
                     /translation="MNEAGTHGPDDRTERVMTTKNLTTADFMEVTSAEGITLVDFWAE
                     WCGPCHRFAPVYERVSEKHPDITFAKVDTEAEQGLAVEFQIYSIPTIMAIRDGVVVYQ
                     QAGALPEHLLENLISKVRELDMDEVRQAAA"
     gene            3752..4141
                     /locus_tag="VFB89_07640"
     CDS             3752..4141
                     /locus_tag="VFB89_07640"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HZM27213.1"
                     /translation="MRPVFLFDGDCSFCSACAGFIARHIPTRAAVVAWQFADLSALGL
                     TKAECDESVQWVEPGRRAAGPAAIAVLLRDSWWHWRVLGRVLGWRPVLALAWPAYRWI
                     SRNRHRLPGGTAVCSLPADQRPPGSPP"
BASE COUNT          704 a         1373 c         1400 g          800 t
ORIGIN      
        1 cgctcggttc ccccggtccg ctccgttcgc cagatcgcct cggttcatcc tgggcggctg
       61 gtgggggcgg agggatgccc gagggatcgg caccgccagg caggtcgcgg tccttgttag
      121 gccgtccggg ttcgctgtcg ggtggtggtt gccatgtcat tggcgagtcc cccttctccc
      181 ctcaacattc ttccctcggc cggtgcattt ggtgagattt cgcacaaggc gtcaccacct
      241 ccgcacattg gccgccaggt cgtcgtgcgc ccatactcac cggtaacgct tttgcgcccg
      301 gaccaactgg caacgtcccg ccacacattc ggcgggtact ttgtggaggc ttcccatggc
      361 tcggctcgcc cagacacccg gtctcaccga ggttcagcgt gacattctca gcaccgtaag
      421 agccttcgcc gaaaaagaga tcatcccgca tgcgcagcgg ctggagcacg cggacgagta
      481 cccgagcgac atcgtcgacg gcatgcgcga gatgggcctg ttcgggctca ccatcgagga
      541 agagtacggc ggcctgggcg agtcgctgct gacctatgtg ctggtggtag aggagctctc
      601 ccgcggctgg atgtcgatct ctgggatcgt caacacccac ttcatcgtcg cgtacctcat
      661 ctcgaatcac ggttcactgg agcagaaggc gcgactgcta ccgaagatgg ccaccggcga
      721 ggtgcgcggc gccttctcga tgtccgagcc aggagtcggt tcggacgtgg ccgggatcca
      781 gtcacgcgcg gtccgcgatg gtgacacatt cgtccttaat ggacagaaga tgtggctgac
      841 caacggcggc tactcgtcgg tcgtggccac actggtcaag accgacctcg atgcgccgac
      901 cgtgtacggc aatatgacaa cgttcctgct cgagaaggag cccggctttg gcgagaccgc
      961 gcccgggctg agcatccccg gcaagctgga gaagatgggg tacaagggcg tcgagaccac
     1021 cgagatgatc ctcgatggac acgtggtacc ggcctcggca gtactcggcg gtgtggacgc
     1081 cgggcttggc cgcggcttct accagatgat ggacggcatc gaggtcggcc gcgtcaacgt
     1141 cgccgctcgc gcgtgcggca tcgccatccg tgccttcgag ctcgccgtgg cctacgcaca
     1201 gcagcgctcc acgttcggca agaagatcgc tgagcaccag gcggtcgcgt tcaagctcgc
     1261 cgaaatggcc acgaagatcg aggctgcgca cgcgctgatg gtgaatgccg cccggctcaa
     1321 ggacgccggc cagcgcaatg acgtcgaggc cgggatggcc aagctgctcg cgtccgagta
     1381 ttgcgccgag gtggtgcagg agtccttccg aatccacggc gggtacggct attcgaagga
     1441 gtacgagatc gagcgcctca tgcgcgaggc cccgttcctg ctcatcggcg agggtacgtc
     1501 cgagatccaa aagacgatca tctcgcgtgg actgctcaag gagtacaagc tctagctgtg
     1561 aggatggcgc ggcgggttcg aatgtggccc agggtcggat tcagacccgc ttggtgagcc
     1621 gctcgatggc gcggatgaca gccgtacaca ggtcctcgac gtagtccatg cctgcctcgg
     1681 cggcgatctc gcgtccttcg gcggagacga tgccctgctg cagccagacc gccttcgcac
     1741 cgatcgccgc ggcgtcgcgt accacggccg ccgcttgccc ggagggtcgg aaaacgttca
     1801 ccagatcgac cggctctggt atgtcgataa gccttggata gcagacctcg ccccagatcc
     1861 gatccgcgta cggattgacc ggaatcatgc gccatccgtg cagcttcagc tgcaatggca
     1921 ccgcgtatgc cgccttctcc tcgtgccgtg acgcgccgac gacggcgatg ttggtggatt
     1981 cttcgagaat ctgcctaggc gtacgcacgc caacgaccct agctcgcgta cgtgcgttcc
     2041 tacgttgctg cgcatcggga acgctagagc agggtgagct gctccgcggc tggcggcgcg
     2101 gccggtgctt cttcgatgcc gcgatgttgc gacatgtgcg ctcgaccgat gccatggcgt
     2161 cgcgcggcta tggcgaccct ggcgctcacc tcccgctggt acgcctttgg cgcgtacgac
     2221 cctttcccgt agatcgaccg ataccggtct accaggtccg gatgagtgcg tccgagccag
     2281 gccgcgtacc actctcgggc gccggggcga aggtgcagga tcaacggcga aacgcccgcc
     2341 gcaccagatg cggcgatcgc agccactgtc gcctcgatgg attcgtccgt gtccgtcagc
     2401 ccgggcagga tcggggccat cagcacgtgt accgggaaac ttgcgtcggc gaagcggcgg
     2461 accgcctcca agcggcgcag cgggctcggt gtgccgggct caacgctgcg ccaggtctcc
     2521 tcgtcgacga agccgacgga gaagctcacg ccaacccggg tgacctcggc tgcctgcctg
     2581 agtaggtcga gatcgcgagt gatcagtgtg cccttggtca ggatcgagaa cgggttggcg
     2641 aagtcgcgca gggcgccgat gatcccgcgc atcaggccgt acttaccttc ggcccgttgg
     2701 taacagtcca cattggtgcc catggcgatg tgggcacccg tccacttcgc cgctgccagc
     2761 tctttgcgca gcagttgcgg ggcattgacc ttcacaacca ccttggtgtc gaagtcgtgc
     2821 cccgcgtcca ggtccaggta cgtgtgagtg ttccgggcga agcagtagac acaggcatgg
     2881 ctgcagccgc gataagggtt gatcgtccac tcgaacggca tccgcgatgc cgatggaact
     2941 ctgctaatga tcgacttcgc ctggatctcg tagaacgtca tgccggcgaa gccgggagag
     3001 tcgaaggtgc gagccacgga accgggaatc gccaggggga gtgcccctgg cgattcgggt
     3061 gcggcgacgc tgagattgtt ccaacgcacg tactaatccg aacacacgta cgggccgttg
     3121 tctacccgtt tccgcgattc gtcttcaaaa gttggcaagc tcgctggcgc tcgccttcgc
     3181 gctcctaggc gatgccgcgt tccagccgtc gtcgcgcgag gctcgctggc gctcgccacg
     3241 cgctcctagg cggctgcctg gcgaacctcg tccatgtcga gctcgcgcac cttgctgatc
     3301 aggttctcga ggaggtgctc cggcagtgca ccggcctgct ggtagaccac gaccccgtcc
     3361 cggatcgcca tgatcgtggg gatcgaatag atctggaact cgaccgcgag cccctgctca
     3421 gcctcggtgt ctaccttcgc gaaggtgatg tccgggtgct tctcggagac ccgctcgtac
     3481 accggagcga agcggtgaca aggaccacac cactcggccc agaagtcgac cagagtaatg
     3541 ccctcagcac tggtgacctc catgaaatca gccgtggtca ggttctttgt cgtcatcact
     3601 cgctccgttc gatcgtccgg cccgtgcgtc ccggcttcgt tcacaacacc cccgcttcgg
     3661 gagccattcc accgcgcccg gatggggtca tgtgcgcagg gtcaccgaac tcggggagcg
     3721 cgggtgcccc gggcgggcgc aggataaggc tgtgcgaccg gtgttcctgt tcgatggcga
     3781 ctgctccttc tgcagcgcct gcgccggttt catcgcgcgg catatcccga ctcgcgccgc
     3841 cgtggtggcg tggcaatttg ctgatctttc ggcactcggg ctgaccaagg ctgagtgtga
     3901 cgaatcggtg cagtgggtag agccgggccg ccgagccgcc gggccggccg cgattgcggt
     3961 gctgttgcga gactcctggt ggcactggcg tgtgctgggg cgggtcctgg gatggcgccc
     4021 tgtgctcgca ttggcgtggc cggcgtatcg ctggatctct cgcaatcgcc atcgcctgcc
     4081 aggcggcacg gcggtctgct ccttgccggc ggaccagcgg ccaccgggat cgccgccata
     4141 acaacgctgt tacgggggca agtaatcgaa ccttacggtt tgataactgt gacgcaggtc
     4201 tctgtctgac ctctattcat ttgcgcactg cggctgcaca attcttgcca tcagagcgtg
     4261 ggcggcgggc gccgggg
//
LOCUS       DASRJQ010000346         4714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3677520, whole genome shotgun sequence.
ACCESSION   DASRJQ010000346 DASRJQ010000000
VERSION     DASRJQ010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3677520"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /gene="pabB"
                     /locus_tag="VFB89_07645"
     CDS             <1..1190
                     /gene="pabB"
                     /locus_tag="VFB89_07645"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HZM27214.1"
                     /translation="GGAAGYIGYDWGATLERLPKTRYDDLHLWDVVLGLYDWVIAWDH
                     CEQAAWIVSTGMPSEVDRIARAQERSSMVRGRLGSGPAKRRAVNGPVAQTQTAPCYPV
                     VGVEGADDIGLRSTFTHRGYLSAVSRVREYILAGDIFQANISQRFQAGLEDEPIQFYR
                     RLRRSNPAAFGAYLDFGEVQVLSASPERFLKVSGRAVETRPIKGTRPRGLGPMHDALL
                     GQVLSESDKDRAENVMIVDLLRNDLSRVCLAGTVRVPELFALEQHPTVHHLVSTVVGT
                     LEPGLDAVDLLRATFPGGSITGAPKVRAMEIIAELEPTQRGIYCGSLGYLSVTGEMDT
                     SIAIRTAVVRNGTVYFQTGGGIVADSEPEAEYRETLDKARGIIRALASARAGASRIAD
                     SSS"
     gene            1187..1879
                     /locus_tag="VFB89_07650"
     CDS             1187..1879
                     /locus_tag="VFB89_07650"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HZM27215.1"
                     /translation="MILLIDNYDSFVYNLARYVRELGETPLVRRHDAVTLDEISALNP
                     SHIIISPGPCSPTEAGLSCDIVRRFGPSIPTLGVCLGHQCVGAAYGGEIARAGRPMHG
                     KTSLIRHNESGIFAGLPNPFAATRYHSLVIAPGSLPPDLEVTATSHDGEIMAVRHRAH
                     PVIGVQFHPESVLTEHGYRMLSNFLGGVRDSLPVGADGGLGLSAIGYRLPAEGMDRRR
                     SPRAHQAPWYVT"
     gene            1906..3741
                     /locus_tag="VFB89_07655"
     CDS             1906..3741
                     /locus_tag="VFB89_07655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HZM27216.1"
                     /translation="MRVPRVLLTLGLTAGSPGIASAQTADSAPQFQRSEMMIPMRDGV
                     RLHTLVFTPARGNDSLPILLTRTPYGIKESEQRLTGAYVELARDGYVFAFQDIRGRYG
                     SEGTFLMNRPLHDPSDSAGVDEATDTYDTVEWLIHNVGRTTGRVGVLGISYPGWLAAM
                     AGVNAHPAVKAISPQAPMTDTWMGDDFFHYGAFRLSYGFEYASEMELTKDGSQPPPVG
                     GYDTYDWYLEQGPLSKLTRLLAGRAPTWTNFTIHPTYDTFWRAHALPTYLKRLTVPTL
                     TVGGWWDQEDFYGPLKTYATLEPLDSGQMNALVVGPWNHGGWSRGDGQKLGEIDFGSP
                     TGRWFREQVQAPWFAHWLKGRDSLPVREAMVFEGGSNRWRTFGRWPPPSRDRNLYFRE
                     NGRLSFDAPTARDPAFDSYVSDPRHPVPYRQRPVQRTYDPRGSGWYTWLTEDQRFVDN
                     RPDVLTWRTEPLTSDVVIAGSLAAQLFAATTGTDADWIVKLIDVYPDSVPGNPKMGGY
                     ELMVASEILRGRYRTGFDRPTAIRAGRIESYTVDLHQQSYRFLQGHRIMVQVQSTWFP
                     VYDRNPQTFVPNIFTARPKDFRAATQRMYRSVSHPSHIALPVLEE"
     gene            3755..4327
                     /locus_tag="VFB89_07660"
     CDS             3755..4327
                     /locus_tag="VFB89_07660"
                     /inference="COORDINATES: protein motif:HMM:NF016199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF447 domain-containing protein"
                     /protein_id="HZM27217.1"
                     /translation="MTLIIESIVTTLDRAGRANFAPMGVEWGEDSIVLKPFLETTTFP
                     NVRDTGVAVVNLTDDVLLFAQSAIGSPVFPSHPATVLRGVVLEAACSWRELEVVNIDA
                     TPPRSRIEMRVCHRGTRRDFIGFNRARHAVLEAAILATRVHLLPAQQIRDELARLAVP
                     VDKTAGPREREAWTLIGGYIDSKIGTVSPP"
     gene            4324..>4714
                     /locus_tag="VFB89_07665"
     CDS             4324..>4714
                     /locus_tag="VFB89_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27218.1"
                     /translation="MTGHGRPDASVYVEAPARLHFGVLDLHGGLGRRFGGMGAAIPTP
                     SLLLEATRAGEVECEGADADRAVGFARRILAHHGQRDGVHLRIHRAIPPHVGLGSGTQ
                     LALAVARAVAELYELPTMTRELARIVDR"
BASE COUNT          820 a         1532 c         1498 g          864 t
ORIGIN      
        1 aaggtggcgc cgccggatac atcggatacg actggggggc gacgctggag cggctgccca
       61 agacgcgcta cgacgacctg cacctctggg atgtggttct gggactctac gattgggtca
      121 ttgcctggga ccattgcgag caggccgcct ggatcgtgtc cacgggtatg cccagcgaag
      181 tggaccggat cgctcgggct caggagcggt cgtcaatggt acgcggccgg ctcgggagcg
      241 gaccagccaa acgccgggcc gtcaacggtc ccgtcgcaca gacgcaaaca gcaccctgct
      301 acccagtggt cggcgtggaa ggcgcggacg atatcgggct ccggtcgacg ttcactcatc
      361 gcggctatct cagcgccgtc tcccgagtgc gggaatacat tctcgccggc gacatcttcc
      421 aggcgaacat ttcgcagcgc ttccaggcag gtctggaaga cgagcccatc caattctacc
      481 gacgcctgcg ccggtccaat ccagccgcgt tcggcgcgta cctcgatttc ggtgaagtcc
      541 aggtgctgag cgcctcaccc gagcgctttc tcaaggtgtc gggcagagcg gtggaaacca
      601 ggccgatcaa gggtacgcgg ccccgcggac tcgggcccat gcacgatgcc cttctcggac
      661 aggtcctgtc ggagagtgac aaggatcggg ccgagaacgt gatgatcgtc gacctgctcc
      721 gaaacgactt gtcgcgcgtc tgcctggccg gcacggtgcg ggtcccggaa ctcttcgccc
      781 tggagcagca tccgaccgtc caccatctcg tctcgacggt cgtgggaacg ctagagcccg
      841 gcctcgacgc agtggacttg ctgagagcca ccttccccgg tggctcgatc acgggggcac
      901 ccaaggtgcg tgcgatggaa atcattgcgg agctcgaacc gactcagcga ggcatatatt
      961 gtggctcgct gggctatctg agcgtgaccg gcgagatgga taccagcatc gcgatccgga
     1021 cggccgtcgt tcgtaacggc acggtgtact ttcaaaccgg cggggggatt gtcgcagatt
     1081 cagagccgga agcagagtat cgtgagacac tcgacaaggc gcgcggcatc atccgggccc
     1141 tggcgagtgc tcgtgcgggc gcgtcgagga tagcggatag ctcgtcatga tcctgttgat
     1201 cgacaactac gattctttcg tctacaacct cgcacgctac gtccgggagc tgggtgagac
     1261 cccgctggta cgtcgccacg atgccgtcac gctcgacgag atctctgcgc tcaaccccag
     1321 tcacatcatc atttcgccgg gaccgtgctc gccgacggaa gccggccttt cctgcgacat
     1381 tgtccggcgg tttggtccct ccatcccgac gctcggcgtc tgcctgggac atcagtgcgt
     1441 cggcgcggcc tacggagggg agattgcgcg ggccgggcgc ccgatgcatg gaaagacgtc
     1501 gctgatccgt cacaacgaga gtggcatttt cgccggactc cccaatccat tcgcggccac
     1561 tcgctaccat tcgctcgtca tcgctcccgg gtcccttccg cctgatctcg aggtcaccgc
     1621 aacctcccat gacggcgaga tcatggcggt ccggcatcgg gctcacccgg tcatcggagt
     1681 gcagtttcat ccggaatcag tgctcacgga acacgggtac aggatgctgt cgaactttct
     1741 gggaggcgta cgcgactctc tgcccgtggg cgccgacggt ggactcggtc tttccgccat
     1801 tggttaccgc cttccagccg aaggaatgga ccgtcgccgc tccccccgcg cccatcaggc
     1861 cccctggtac gtgacttagc ccgccatgaa cggactctga tcagaatgcg cgtgcctcgt
     1921 gtgttgctga cgctcgggct cacggccggc tcgccgggca ttgcgtcggc gcagacggct
     1981 gactctgccc cgcagttcca gcgatccgag atgatgattc cgatgcgcga cggcgtccgg
     2041 cttcacacgc tggtattcac acccgcccgc ggcaacgact cgctcccgat tctgctcacc
     2101 cgtacaccgt acgggatcaa ggaatcagag caacgactga ccggagcgta cgtcgagttg
     2161 gctcgcgacg gttacgtctt cgcgtttcag gacatccggg gacgctacgg gtcggaggga
     2221 accttcctga tgaaccggcc gctccacgat ccgagcgact ccgccggcgt ggacgaagcc
     2281 acggacacct acgataccgt cgagtggctg attcacaacg tcggcaggac gacgggcagg
     2341 gttggggtgc tcggtatttc ctatccaggc tggctggctg cgatggcggg cgtaaacgcg
     2401 caccccgcag tcaaggcgat atcgccccag gcaccgatga cggatacctg gatgggtgac
     2461 gacttctttc actatggtgc atttcggctg agctacggtt tcgaatacgc ctcggagatg
     2521 gagctgacga aggacggctc gcaaccaccc cccgtgggcg gctacgacac ctacgactgg
     2581 tatctcgagc aggggccgtt gagcaagctg acgcggctcc tggccgggcg cgcgccgacc
     2641 tggaccaatt ttacgatcca tccgacgtac gacacattct ggcgcgctca cgcgctgccc
     2701 acgtacctga aacggctcac ggttccaacg ctgactgtgg gcggctggtg ggaccaggaa
     2761 gacttttacg gcccgctcaa gacctatgcg accctcgagc cgctcgattc cgggcagatg
     2821 aatgcgctgg tggtcggccc ctggaatcac ggtgggtggt ctcgaggaga cggacagaag
     2881 ctgggcgaga tcgatttcgg cagcccgacc ggccgctggt tccgggaaca agttcaggcc
     2941 ccctggttcg cccactggct gaagggccga gattcgcttc ccgtgcgcga ggctatggtg
     3001 ttcgagggcg gatcgaatcg ctggcgcacg ttcggccgct ggccgccgcc cagccgcgat
     3061 cgcaacctct acttccggga aaacggacgg ctgtcgttcg acgcgccgac tgctcgcgat
     3121 ccggctttcg atagctacgt atccgatccc cggcaccccg taccgtaccg acagcgaccg
     3181 gtacagcgga cctatgatcc ccggggatcc gggtggtaca cgtggctcac ggaggaccag
     3241 cgattcgtcg ataaccgccc ggacgtgttg acctggcgta cggagcccct gacgagcgac
     3301 gtggttatcg ccggtagcct agcagctcag ctatttgcgg cgaccacggg gaccgatgcc
     3361 gactggatcg tgaagctgat cgatgtatat ccggattcgg tgcccggcaa tccgaaaatg
     3421 ggcggctacg agctgatggt cgccagcgag atcctgcgcg gacgttaccg caccggcttc
     3481 gatcggccga ccgcaatcag ggccgggcgc atcgagtcct acacggtcga cctacaccag
     3541 cagagctatc gctttcttca agggcatcgg atcatggtcc aggtccaaag cacctggttt
     3601 cccgtctacg atcgcaatcc tcagacgttc gtgcccaaca ttttcaccgc tcggccgaag
     3661 gattttcgcg ccgcgacgca gcgtatgtat cgatcggtct ctcacccctc gcacatcgcc
     3721 ttgccggttc tggaggaatg atcgagagcg attcatgacg ctgattatcg agtcgatcgt
     3781 cacgaccctc gaccgggcag gtcgggccaa tttcgcgccc atgggcgtcg agtggggcga
     3841 ggactcgatc gtgctcaagc cttttctgga aaccaccaca ttccccaacg tccgagatac
     3901 cggtgttgcg gtagtcaatc tgacggacga tgtgctgctc ttcgcccaga gtgcgatcgg
     3961 atctccggtc ttcccttcgc acccggcgac ggtgctgcgc ggcgtcgtgc tcgaggcggc
     4021 gtgctcctgg cgggagctcg aagtcgtcaa catcgacgcg accccgccga gatctcgaat
     4081 cgaaatgcgg gtgtgtcacc ggggaactcg ccgcgacttt atcggattca atcgggcccg
     4141 ccatgcggtg ctcgaagctg ccattctcgc cacccgcgtg cacctgctgc ctgcgcagca
     4201 gatccgcgac gagttggctc gcctcgccgt cccggtggac aagacggcgg gaccacgtga
     4261 acgcgaggcc tggacgctga tcggcgggta catcgactcc aaaatcggca cggtgtcacc
     4321 gccatgactg gccacggccg gcccgatgcc agcgtttatg tggaagctcc cgccagactc
     4381 cactttggcg tgctggacct gcatgggggg ctgggtcggc gattcggcgg catgggagcg
     4441 gcgatcccca ctccctcgct gttgctcgag gcgacgcgcg ccggtgaggt cgaatgcgaa
     4501 ggagccgatg cggaccgtgc ggtcggcttt gcccggcgca tcctggctca ccacggccag
     4561 cgggacggcg tgcatctccg catccatcgc gcgattccgc cccatgtcgg actcggctcg
     4621 ggcactcagc tcgcgctggc ggtggcgcgg gccgtggcag agctctacga gctccccacc
     4681 atgacccgcg agctcgcccg aatcgtggat cgcg
//
LOCUS       DASRJQ010000347         4122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3718106, whole genome shotgun sequence.
ACCESSION   DASRJQ010000347 DASRJQ010000000
VERSION     DASRJQ010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3718106"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(404..1288)
                     /locus_tag="VFB89_07670"
     CDS             complement(404..1288)
                     /locus_tag="VFB89_07670"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HZM27219.1"
                     /translation="MTPQQLTAPAAVPDRKPRDDEIDIHGLTHPGKVRTENQDHFLVC
                     ALRRQTVVQLTSLPDPDRLMGESERVGLLMMVADGVGRGSKGGEASRIALEGVARYVS
                     GSMRCYYAAGSSDDREIFEALQEGALQCHAELLRRGEEEPEYHGMATTLTLYLGAWPR
                     AYLLQAGDSRCYLLRRGELTQITRDQTMAQELIDLGVLSRADASSSRLSHTLSSSIGG
                     RQSAPVVTRIGLDWDTVVLLCSDGLTRHVTDERIRNRLRSMSSARQVCEDLVQDALDA
                     GGQDNITVVVRRTVIKGG"
     gene            1759..2235
                     /locus_tag="VFB89_07675"
     CDS             1759..2235
                     /locus_tag="VFB89_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27220.1"
                     /translation="MAHVRPAGTQLVAIAMLAGACSRSEESRPAGAETGVTDSAALAA
                     SASSSGKLRVSNVMIGRQVGRGNLITQPTFEFAPQDTIHVSVATEGSGGTSTLTAAWR
                     SQSGQILQQTSEPVRHAGENIAFRLSQPKGLKPGTYKVIVFLDDDSVETKVFVVRK"
     gene            2682..3131
                     /locus_tag="VFB89_07680"
     CDS             2682..3131
                     /locus_tag="VFB89_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZM27221.1"
                     /translation="MTAKPACCTPQDTVRTAAQLMREHDCGCIPVVENEGSNRAVGVI
                     TDRDIACRCTADGKGPETLVKDVMSRDPTCCGSDDDVDAVERIMIEKQVRRVPVVDSE
                     GCCVGMIAQADLVLNHGAASEREVRTIVERISEPAHHSGAAPSLGGR"
     regulatory      3212..3328
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            3672..>4122
                     /locus_tag="VFB89_07685"
     CDS             3672..>4122
                     /locus_tag="VFB89_07685"
                     /inference="COORDINATES: protein motif:HMM:NF018180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HZM27222.1"
                     /translation="MTATSTIQQRSERAADKNAIRPFQVKVRDADLTELRRRIQATRW
                     PDRETDASQGVQLATMQELARYWATEYDWRKVEARLNALPQFITEIDGVDIHFIHVSS
                     KHEDALPLIVSHGWPGSVIEQLKIIDPLTNPTAHGGKVSDAFHVVIPN"
BASE COUNT          770 a         1290 c         1333 g          729 t
ORIGIN      
        1 ctggttttgc ggccccggat cgctccgggt gtgctctttt cgggtgacgt cgattgtctg
       61 cccgcgctac aggtggcgca ggcgggtcag tcttcttgca attaccaggc tcaatggtgc
      121 tgcaggctgt tgcattgggc ggcagaattg cagcggcatc cgggcccggc ggtaagatcc
      181 gcgccgctat gccagaatca agttgctcat tcgcaaagcc gagcggcccg tacaaaattg
      241 cgtggagtga cgcgtactga ttgaccgagg tgcatagaat agcgacggca accggcgctc
      301 cgaaaccgca atgaacttgt ctgcagcgtt gcgacagcgc cgaaaacggc ggtcctctgc
      361 aacattgtgc cgccacatct gagacaaccc ttccggatac cgctcatccc ccctttatca
      421 ccgtccggcg caccacgacc gtgatgttgt cctgcccacc ggcgtcgagt gcgtcctgca
      481 ccaggtcctc gcacacctga cgggccgacg acatcgagcg cagccggttc cggattcgct
      541 cgtcggtaac gtggcgggtc agcccgtcgc tgcagaggag cacgacagtg tcccagtcta
      601 ggcctattcg ggtgacgact ggcgcactct gtcgcccgcc gatcgagctc gacaacgtgt
      661 gcgagagccg gctgctcgat gcgtcggccc ggctcaacac gcccaaatcg atcagctcct
      721 gggccatcgt ctgatcgcgg gtgatttggg tcagctcgcc gcgccgaagg agatagcacc
      781 ggctgtcgcc cgcctggagc aagtaggccc gcggccaggc accgaggtac agggtaagcg
      841 tcgtcgccat gccgtggtat tccggctcct cctccccgcg ccggagcagc tccgcgtggc
      901 actggagcgc cccctcttgc agcgcctcga agatctcccg gtcgtcggac gagccggcgg
      961 cgtagtagca gcgcatgctg ccggacacgt atcgcgctac gccctccagt gcgatccggc
     1021 tcgcctctcc gcccttgctg ccgcggccga cgccatcggc caccatcatc agcagcccta
     1081 ctcgttcgct ctctcccatg agtcgatccg ggtccggcag gctggtgagc tggaccaccg
     1141 tctgtcgccg cagcgcgcac accagaaagt ggtcctggtt ctcggtgcga actttcccgg
     1201 ggtgggtcaa tccgtgaatg tcgatctcgt cgtcgcgtgg cttccggtct gggacggcgg
     1261 cgggcgcggt taactgctga ggagtcatcg gggcctcctt tttcaagggg cctgagggac
     1321 catgccggcg gccacgaagg catcgggccg aaatgttcat tcccaatctg cgggatgcta
     1381 cgacgttctg ccagattcgg gtgccgcccc agttcaaacc tcattagctc gggcggtgcc
     1441 actcggccac aaccacgggt cacggtggcg gtggcgtggt cgggggttct cctgaagaag
     1501 ggggatgcgc gacggacgcg cctccatcac gcagagccgc gccgccaatt ctgagctacc
     1561 tcaacgttcc gcgggaactc cgggctcgcc ggggcgtcac tgcttctgct cgtgaccgct
     1621 tcccgctcat gcccgggtct attggcgtga ggcgaccaaa accttaccat tgtgaaagac
     1681 tgctgcggat cggaggcggc ttcgcgtagc ctgaagcgcg gaggtgaaga agtagccttg
     1741 catgacgcgg aggtgtttat ggctcacgtt aggccggccg gcacgcagct ggtggcgatc
     1801 gccatgctcg ccggcgcttg ctctcggtcg gaggaatcgc gcccggcggg agccgagacg
     1861 ggagtgaccg actcggccgc tctcgcggcg tccgccagca gctcgggcaa gctgcgggtc
     1921 tcgaatgtaa tgatcggaag acaagtgggg cgtggtaacc tgattaccca gccgacgttc
     1981 gagttcgctc cccaggatac gatacatgtt tcggtggcta ccgaaggcag cggcgggacc
     2041 agtacgctga cagccgcttg gcgatcccag agtggccaga tcctgcagca gacctctgaa
     2101 ccggtgcgcc acgcgggtga gaatatcgcg ttccgattgt ctcagcccaa gggcctgaag
     2161 cctgggacct acaaggtgat cgtgtttctc gacgacgact cggtagagac caaggtcttc
     2221 gtggtgcgta agtagcgagg cgcgcttgtt atggctggcc gagctcggcc cgtcaacacc
     2281 aaccggtgaa ggggtccaac cctgcgcgct gatatcgcgc ccggaggcac ctttcccgac
     2341 tacgagctgc ccgatcgcac gagcacctca cgcaggttca gcgacctgca gggcgacgac
     2401 ccactgacct tccgatcgtc cggcgcgccc ccggcggggc attagagaaa cggtagccga
     2461 tgtggagcag gtcccggcca cggggacctg cagcgcgccg cgcttgccga ccagccgtcc
     2521 cctcgcacgt cgccgaaaga ggcagcgcca acgtgaccgg catcaccgct tgcgactgtc
     2581 ccagtatcga ttctccgctc aaaagcaaag tggttatacc accggggact gttcctgtgc
     2641 ccctacggag atgggagggc aatgtgaaag ctggagaatt gatgaccgcc aagcctgcct
     2701 gttgcacccc gcaggatacc gtccgcacag ccgcccagct catgcgggag cacgactgcg
     2761 gctgcattcc agtggtagag aacgagggga gcaatcgagc ggtgggcgtc attacggacc
     2821 gtgacattgc ctgccgctgc acggcggatg gcaagggacc agagaccctt gtgaaggacg
     2881 tcatgtcgcg cgatccgacg tgctgcggct ccgacgatga cgtggacgcg gtggaaagga
     2941 ttatgatcga gaagcaggtc cggcgagtgc cggtggtgga ttcagagggc tgttgcgtgg
     3001 ggatgattgc gcaagcggat ctggtcctca accacggtgc ggcgagcgaa cgcgaggtcc
     3061 gaaccatagt ggagcgcatt tcggagccgg cacatcattc cggcgcggcg ccttcccttg
     3121 gcgggcggta gttcgagcag aattccgtaa cagatgagca accttgccgc gcttgaccgc
     3181 tccgatcccc aggcctaggt tcgagctgac ggaacgatca atgcacaccc ggtgaaagcc
     3241 ggcgtcgcaa agccacgggt cctcgcctcc tcggcgacac cggagtttag gacgccacga
     3301 gggacctcaa gaccgccgag ctgcatgaag gtcagctcgg cggtcgtgct ttttgctgac
     3361 gacgggcgcg acgtgcagac gagacgagca gcggcgccgt caccgcacca aggagaaagg
     3421 agaccccatg tctcactggg taacgacctc gagtgctctc ggccttcttc tcatcgcttc
     3481 cccagctacg ccgagaccgt cgcccttcgc aagcgagctt tgcacggcgt tcagatcact
     3541 gcgctagggc ggtgcctgcc atgggcgcag cgaagggacg gaggacatag agaagacgga
     3601 gtgcgggagt ttggcgttac ggcgccgagc agcggttgaa ggaactcaca ggaactcaca
     3661 aggaagacac catgactgcg accagcacta tccaacaacg cagcgagcgg gcagctgaca
     3721 agaatgccat tcgtccgttt caggtgaagg ttcgggacgc ggaccttacc gaattgcgca
     3781 ggcgcataca ggcgacaagg tggcctgacc gggaaacgga tgcatcccag ggcgtgcagc
     3841 tcgcgacgat gcaggaactc gcgcgctatt gggcgacaga gtacgactgg cgcaaggtcg
     3901 aggcgaggct gaacgcgcta ccgcagttca tcacggagat cgatggggtc gacattcact
     3961 tcattcacgt tagttcgaag cacgaagacg cgttgccgct catcgtctcg cacggatggc
     4021 ccggctcggt catcgagcag ctgaagatca tcgatcccct gaccaatccg acggcgcacg
     4081 gcggcaaggt atcggacgct ttccatgttg tgattccgaa ca
//
LOCUS       DASRJQ010000348         2757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2551308, whole genome shotgun sequence.
ACCESSION   DASRJQ010000348 DASRJQ010000000
VERSION     DASRJQ010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2551308"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(210..1841)
                     /locus_tag="VFB89_07690"
     CDS             complement(210..1841)
                     /locus_tag="VFB89_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27223.1"
                     /translation="MALLSCAAVSTLSLAVGLPTLQAHNYFLADDFGLVHHLHNLALS
                     RFLPYFVSDWTEGIYGYTLDELRPVLAFSYWLDSHMFGATNAMGYHATNVALHVLNSV
                     LVFAIARAIVPAQTAIPLLAGAVFALLPSSAEPIAWISGRVDSLTSAFYLGAFFGFLR
                     FRIRHSRPWLAASLVLFAFGLFVKQTVVTFPVLILAYDAVYQPSIRSSTRHELISRYA
                     VQVPFLLLLVLYLGLRATLFDSALRGEQVTLEAFRVFAIRQFFYARSLLPIAYQIPDQ
                     AKVALAVFTAGVLVACGRWLLNRDVRDPDVRRHLLFFGVIWYGVTIAPMIVTYPSARH
                     LYLTGAGVSIALALALLPEWWRDASRSMVPRALSVVVFLVLSGIALTLNIRTWIANGV
                     ESGRLSASLPVLLRSLPSGSTVMVGIPETRRDAYFWYFALPFALQKPFLPEDLYGQFA
                     IVERPGVYCCLPHQWWAAKRSTLEPILRSSGTRELTYIVPDPRDAGAWRVQKRTVSGP
                     ELRMKIENTVGKRLESLAISTSWTDVYPLAKILFE"
     gene            complement(2019..>2757)
                     /locus_tag="VFB89_07695"
     CDS             complement(2019..>2757)
                     /locus_tag="VFB89_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27224.1"
                     /translation="RDSGLGTRAPGLGTRAPGATHPPTAALFSRRACDTDAMLMNRYA
                     LGGVWRGMRAPGMRPTWVLVLLLAAGPVAGQVKDRVLQVNFTPSDDSIRAATDEYKAI
                     WGQEGPRIVPAMERRSGLRFEAGPIEVSVYEGTSYSGEPGGRPMLLRASYPAATKRAT
                     LVHELAHRLANDVRVPFDHHEVIFLFVYDVWVELWGQMFADEQVVIESRRTGADYAGM
                     WKKVLAMSPAERAGRLKAVIAEYGTAR"
BASE COUNT          570 a          860 c          882 g          445 t
ORIGIN      
        1 ggcaaccggc ctggagcggc tgaacctgtc gacgatcacc tcgccgacat tccagggaac
       61 gttcgatcgc gacgatcagc tgtcccgggc gcaggcgcag ttcggcgcgc gaatcaggtt
      121 ctgattgcag gagcccgatg cgcgtcgcgc tgagggctcc cgttgatcgg tttgacagac
      181 ctgttccagg ccacgctgtc ggagtccctt cactcgaaga gaatcttggc cagcgggtag
      241 acgtcggtcc aggaggtgga aatcgccagg gactcgagtc gtttccccac tgtattctcg
      301 atcttcatgc gcagctctgg accgctcacc gtccgtttct ggactctcca ggcgccggca
      361 tcccgcgggt caggcacgat gtacgtcagc tcgcgtgtgc cggacgagcg cagtatcggc
      421 tcaagggtcg atcgtttcgc tgcccaccac tgatgcggca ggcagcaata cacccccggc
      481 cgctcgacga tcgcaaactg tccgtacagg tcttcgggca ggaacggctt ctgcaatgcg
      541 aacgggagtg cgaagtacca gaagtaggcg tcacgacggg tctcggggat tccaaccatg
      601 accgtgctgc ctgacgggag cgatcgaagc aggacgggca gtgaagccga gagccttccc
      661 gactcgacgc cgttggcgat ccatgtccgg atgttcagcg tcagcgcgat cccggaaagc
      721 accaggaaca ccacgacgga cagggctcgg ggaaccatgc tccgggaagc atcgcgccac
      781 cactcgggca agagcgccag tgccagggcg atactcactc cagcgccggt caagtagaga
      841 tgacgcgccg aaggataggt gacgatcatc ggcgcaatgg tcacgccgta ccaaatcacg
      901 ccgaaaaaga gcagatgacg acggacgtcc gggtcgcgaa cgtcccggtt gagcagccat
      961 cgtccgcacg ccaccagcac tccagccgtg aacaccgcta gcgcgacttt cgcctgatcg
     1021 ggaatttggt aggcgattgg taatagcgac cgtgcataaa agaattgccg gatagcgaac
     1081 actctgaacg cctccagggt cacctgttct ccccgcaggg cgctgtcaaa gagtgtcgcc
     1141 cgaagcccga gatagaggac cagcagcagc aggaacggca cctgaacggc atatcgtgaa
     1201 atcagctcat ggcgcgtcga gctcctgatg ctcggctggt acacggcgtc gtacgccagg
     1261 atgagtaccg ggaaggtcac gaccgtttgc ttgacaaaaa gaccgaaagc aaacaggacc
     1321 aacgacgccg ccagccaagg gcgggagtgc ctaatacgga agcgaagaaa accgaagaac
     1381 gcacccaggt aaaacgcgga tgtgagcgag tcgacccggc cactgatcca ggcgataggt
     1441 tccgcactcg aaggcagcag tgcgaacacg gcgccagcca gcagcgggat ggcggtctgt
     1501 gccgggacga ttgcgcgcgc gatggcaaag accagcacgc tgttgagcac atgcagagcg
     1561 acgttcgtgg cgtggtaccc catcgcattc gtcgctccga acatgtggct gtctagccag
     1621 tagctgaaag ccagcacagg ccgaagctcg tccagcgtgt agccgtagat gccttcggtc
     1681 caatcagaga cgaagtacgg aaggaatcgg ctaagcgcta ggttgtgaag atggtggacc
     1741 agtccgaaat cgtcggccag gaagtagttg tgcgcctgaa gtgtcggcag accaacggca
     1801 agactgagtg tcgaaactgc ggcacatgag aggagagcca cccttgtccg gcgagccgag
     1861 ttgtcagacg gcgtacggct aatgatcggc gctttcactg cgaatgttgg ggtggagggg
     1921 aacgcgcacc ctgcggaggg cttgagacaa agggcggtga tgatatcacc aacctataca
     1981 gtccaggcag cgcgaggcgg ggaactccgg cagacgtttc agcgcgccgt gccgtactcc
     2041 gcgatgaccg ccttgagtcg acccgcccgc tcggcgggcg acatggcgag gaccttcttc
     2101 cacattccgg cgtagtccgc cccggtgcgc ctgctctcga ttacgacctg ttcgtcggca
     2161 aacatctgtc cccagagctc gacccagacg tcgtacacga acaggaagat cacctcgtgg
     2221 tgatcgaacg gcactcgcac gtcgttcgcc agccgatgcg cgagctcgtg cacgagggtt
     2281 gcacgcttgg tcgcggcagg atagctcgcg cgcagcagca tcggccggcc tccgggctcc
     2341 cccgagtagc tcgtgccttc gtaaacgctc acctcgatgg gcccggcttc gaagcggagc
     2401 cctgagcgcc gctccattgc cgggacgatg cgcgggccct cctgccccca gatggctttg
     2461 tactcgtcgg tcgcagcgcg gatggagtcg tccgacgggg tgaagttcac ctgcagcact
     2521 ctgtccttca cctggcccgc caccggaccg gccgccaaca gcaggacaag cacccacgtc
     2581 ggtcgcatgc cgggagctct catgccacgc cagacgccgc cgagggcgta tcggttcatg
     2641 agcatcgcat ccgtatcgca ggcgcgccgg ctgaagagcg ccgccgtcgg cgggtgtgtc
     2701 gcgccagggg cccgagtccc gagtcccggg gcccgagtcc cgagtcccga gtcccga
//
LOCUS       DASRJQ010000349         4506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3979050, whole genome shotgun sequence.
ACCESSION   DASRJQ010000349 DASRJQ010000000
VERSION     DASRJQ010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3979050"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VFB89_07700"
     CDS             complement(<1..620)
                     /locus_tag="VFB89_07700"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HZM27225.1"
                     /translation="MSDPESESTYLLVVGAGPCGLAVGVAAKQAGIPCILLDRRNIVS
                     TIERYPLQMTFFSTPERIEIGGVPFVASHEKPTRRDGLIYYRRVAEHYALDIRPGEEV
                     VDALREGDGSFRIEVSRRHDSKVYQATALVFATGYFDNPNLLRVPGEDLPHVAHYFTE
                     GHPYWHQRVVVIGAGNSSVDAALECWRAGAHVTIVHFGDALDRTVKP"
     gene            complement(710..1990)
                     /locus_tag="VFB89_07705"
     CDS             complement(710..1990)
                     /locus_tag="VFB89_07705"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HZM27226.1"
                     /translation="MFDARTACVVVVGAQWGDEGKGKLVDVLAERADFVVRYQGGANA
                     GHTVVTGQTQFILQQIPSGILHPRVICIVGNGVVLDPDQLFTELDQLAAKGIDPSNRL
                     FVSDRAHLVLPYHKQLDRASERSQNIGTTGRGIGPTYEDKYGRRGIRVGDLRQLDCMR
                     PVLAERVERANRLLELMGSTERVSLESEMALLERWAPRLLPLTVDSGLMVYRAIKDGK
                     RVLLEGAQGSLLDVDHGTYPFVTSSNTTAGGAAVGAGIGPTAIHGVLGVVKAYTTRVG
                     NGPLPTEAVDDSGAKLRELGGEFGAVTGRPRRCGWFDATVVRYAVRVNGLTGLAVTKL
                     DVLDGFTEIPVGIAYRLDGSSCAEMPSEVDLLGRVQPMYETLPGWNRPTGEARKLSDL
                     PVEARRYLDRLQELSGTPIRYVSVGTRRDQIIEV"
     gene            complement(2071..3186)
                     /locus_tag="VFB89_07710"
     CDS             complement(2071..3186)
                     /locus_tag="VFB89_07710"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HZM27227.1"
                     /translation="MASRLHPLDPALRDVLTRPREAGTADAAAAREVVAERLGALGFT
                     VETHHFRFHPSTLLGFPLLGAGLGWLGLVLIPLLVSVQVPPWAAGAAWLMGCASLAAL
                     AYGIGSGAAPLRVGDFRDDANLLATRSRDVRGWIVAHVDTKAQGHSMAGRLIAVWVVM
                     AAVAEQTVLVVLRLSGPLPLSAATMGALLGVVAGMLAGKGRLAGQSPGARDNGSGVAA
                     ALSAAAALSDPGIGIMITGAEEFGLLGARALVRDRPGLFAGRIVVNFDTLDDTGSLQV
                     VSHDLPGHAAAQAAAAALAADGLATKLRRLPLGILVDSLPFARAGARAVTVARLDWST
                     LQRLHTVRDSIDGCSFETALHVGEIIARRFDVFDAAS"
     gene            complement(3161..4240)
                     /locus_tag="VFB89_07715"
     CDS             complement(3161..4240)
                     /locus_tag="VFB89_07715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HZM27228.1"
                     /translation="MEKIIKAAVERGASDLHIKAGDVFRARINGKLVALTKQRLTPDQ
                     TKAIALKLMSSDTDRARIDQVRDFDCSWGMPGVGRFRVNVLRQRSSFMIVMRVIPFTV
                     PSIESLALPDSLKAVAEAERGLIVVAGVSGSGKSSTVAAMVHHINKTQHKHIVTVENP
                     IEFLHRDLSCSVTQREVGVDTDALATGLRAALRQDPDVVVIGDIADAITVDAAIKAAE
                     TGRLLIATLPTPDVTSTIERILATLPTEEREIGRMRFAEAVRAVLAQQLLPRKDEKGR
                     TAAMEILIATPAVRECLKSSTRFAELKKIMSDATDEGMQTFEQHLNDLVSAGRLSPES
                     AKTALIPTSMSGSQPGKRGKQAASA"
     gene            complement(4269..>4506)
                     /locus_tag="VFB89_07720"
     CDS             complement(4269..>4506)
                     /locus_tag="VFB89_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27229.1"
                     /translation="NCRGAKWGCLDCKRVLADAITTHFAPIRERARELQAHPARVEEI
                     LASGAMRARAIARETLQQARELMGFLPAVAPVND"
BASE COUNT          817 a         1517 c         1405 g          767 t
ORIGIN      
        1 ggcttcacgg tgcgatccag cgcgtcgccg aaatgcacga tcgtgacgtg agcgccggct
       61 cgccagcact cgagtgcggc gtccacggaa ctgttcccgg ccccgatcac cactacgcgc
      121 tgatgccagt aagggtggcc ctcggtgaaa tagtgcgcga cgtgcgggag atcctcgccc
      181 ggcacccgca gcagattggg attgtcgaag tagccggtgg cgaacaccaa cgccgtcgcc
      241 tgataaacct tggagtcgtg ccggcggctg acctcaatgc gaaaacttcc atccccctcc
      301 cgtagcgcat cgacgacctc ttcgccagga cgtatatcca gcgcgtaatg ctcggcgacg
      361 cgccgatagt agatcagccc gtcccgccgg gtgggctttt cgtggctggc gacgaacggc
      421 acccctccga tctcgatccg ctccggagta gaaaagaacg tcatctgcag cggatatcgc
      481 tcgatggtcg agacgatgtt gcgccggtcg aggagaatgc agggaatgcc ggcctgcttg
      541 gcggcaacgc cgacagcgag gccgcatggc ccggcgccga cgacgaggag ataagttgat
      601 tcactctcgg gatccgacat tactcctcgg aactgcgtaa accgtgaacg gtaaacggga
      661 ggtttgatga tgaacggcct cccgttcacc gttcaccgtt tacgcttttc tagacttcaa
      721 taatctgatc tcgcctcgtc cccacgctca cgtaccggat cggggtcccg gaaagttcct
      781 gaagccggtc caggtaacgc cgagcttcga ctggaagatc gcttagcttg cgggcctcgc
      841 cggttgggcg gttccagccc ggcaacgtct cgtacatcgg ctggacccgg cccagcagat
      901 ccacttccga aggcatttcg gcgcaggacg acccgtccaa acggtaggcg atgccgaccg
      961 ggatttcggt gaacccatcc aagacgtcca gcttggtgac cgcaagaccg gtgagaccgt
     1021 tcacgcgcac ggcatatcgt acgacggtcg cgtcgaacca cccgcagcgg cgcggccgtc
     1081 ctgtcaccgc gccgaactcg ccgcccagct cccgcagctt ggccccggaa tcgtccacag
     1141 cctcggtagg tagcggcccg ttgcctactc tcgtcgtgta tgccttcacg acgccgagca
     1201 cgccgtgtat cgcggtcgga ccgatgccgg cgccgaccgc ggcgccgccg gccgtcgtgt
     1261 tggacgaggt gacgaaggga taggtcccgt gatcaacgtc gagcagcgag ccctgggcac
     1321 cctcgagcaa gacccgtttg ccgtccttga tcgcgcgata gaccatcagt ccgctgtcga
     1381 ccgtgagcgg cagcaatcgt ggggcccagc gctcgagcag cgccatttcg ctttcgagtg
     1441 acactctctc cgtgctgccc atcagctcga gcaggcggtt ggcgcgctcg acgcgctcgg
     1501 ccaggaccgg ccgcatgcag tcgagttgcc gcagatcgcc cacccggatg ccgcgacggc
     1561 cgtacttgtc ctcgtaggtg ggaccgatgc cacgccccgt ggtcccgatg ttctggctgc
     1621 gctcgctcgc ccggtcgagc tgcttgtgat agggaagcac cagatgagct cgatccgaca
     1681 cgaagagccg gttgctcgga tcgatcccct tggccgccag ctggtccaac tcggtgaaca
     1741 attggtcggg atccagaacg acaccgtttc caacaatgca gatgactcgg ggatggagaa
     1801 ttcccgaggg gatctgctgg agaatgaact gcgtctggcc ggtgaccacc gtatgcccgg
     1861 cgttggcccc accctgatac cggaccacga aatccgcccg ctccgccagc acatccacga
     1921 gcttgccctt gccctcgtca ccccattggg caccgacgac tacgacgcaa gcggttcgcg
     1981 cgtcgaacat gaacgtcctc cggcaggtca acgggacacc ccacgccaac aaaaaaaccc
     2041 cgcgcggggt tcgggaatcc ttacacgaaa ctagctggcg gcatcgaaaa cgtcaaagcg
     2101 ccgagcgatg atttcgccga cgtgcagggc agtctcgaac gagcagccgt cgatcgagtc
     2161 acgcacagtg tgcaggcgct ggagagtaga ccagtcgagc cgggcaaccg tgactgctcg
     2221 cgctcccgcg cgggcaaaag gaaggctgtc caccaggatg cccaggggca gccgccgcag
     2281 tttcgtcgcg agaccgtcgg ccgcgagcgc cgcagccgcg gcctgcgccg cggcgtgccc
     2341 cggcaggtca tgactcacca cctgcagcga gccggtgtcg tcaagtgtgt cgaaattcac
     2401 cacgatgcgc ccggcgaaca ggcccggtcg atctcgcacc agtgcgcggg cgcccagcag
     2461 accaaactcc tccgcaccgg tgatcatgat cccgattccc ggatcactga gagcggcggc
     2521 tgccgataga gcggccgcga caccgctgcc gttgtcccgt gcaccagggg attgacccgc
     2581 gagccgcccc ttccccgcca acatgccggc aaccacgccc agtaatgcgc ccatggtcgc
     2641 cgccgacagc ggcagcggcc ccgagagtcg gagcacgaca agtaccgtct gctccgccac
     2701 cgccgccatg accacccaga ctgcaatcag gcggcctgcc atcgagtgcc cctgcgcctt
     2761 ggtatccacg tgggcgacga tccagcctct gacgtcgcgg gaccgggtgg caagcagatt
     2821 ggcatcgtcg cgaaaatcgc cgacccggag cggcgccgcc ccggacccga taccataggc
     2881 cagtgctgcg agactggcac agcccatcag ccaggcagcg ccggccgccc acggaggaac
     2941 ctgcaccgac accaggagtg gaatcaggac gaggccgagc cagccaagac cggccccgag
     3001 cagaggaaaa cccaggagag tggagggatg gaaacggaag tgatgggtct cgacagtgaa
     3061 ccccagggca cccagccgtt cagcgacgac ctcgcgggcg gcggctgcat ccgccgtacc
     3121 cgcttcgcgc ggacgagtca ggacatcccg gagcgccggg tcaagcggat gcagcctgct
     3181 tgccacgctt gcccggctgc gatccagaca tggaggtggg aatcaatgcg gtcttggcac
     3241 tttcgggact caatcgtccg gcgctgacca ggtcgttcag gtgctgctcg aacgtttgca
     3301 tgccctcgtc ggtggcatcg ctcatgattt tcttcagctc ggcgaagcgg gtagacgatt
     3361 tgaggcactc gcgcacggcc ggcgtggcga taaggatttc cattgcggcg gtgcggcctt
     3421 tctcgtcctt gcgcggcagg agctgctgag ccagcaccgc gcggacggct tcggcaaacc
     3481 gcatccgccc gatttcccgc tcttcggtcg gcaaggtcgc gaggatgcgc tcgatggtgc
     3541 tggtcacgtc cggagtgggc aacgtggcaa tcagaagtcg gccggtttcc gccgccttga
     3601 tggcagcatc gaccgtaatc gcatcggcga tgtcgccgat caccacgacg tcggggtcct
     3661 gccgcagcgc cgcccggagc ccggtggcca gcgcatccgt gtccacgccg acctctcgct
     3721 gggtcactga gcagctcaag tcccgatgca ggaactcgat tgggttctct acggtcacga
     3781 tgtgcttgtg ctgagtcttg ttgatgtggt gcaccatcgc cgccaccgtc gagctcttgc
     3841 cgctgccgct gacgcccgcc acgacgatca gtccgcgttc cgcctcggcg actgccttca
     3901 gggagtcggg taatgcgagc gattctatcg atgggacggt gaatgggatc acccgcatca
     3961 cgatcatgaa gctcgaccgc tggcgcagca cgttgacccg aaatcttccc accccgggca
     4021 tcccccagga gcagtcaaag tcgcgcactt gatcgattcg ggcacgatcc gtgtcgctgc
     4081 tcatcagctt gagcgcgata gccttcgttt gatcaggagt gagccgttgc ttggtgagcg
     4141 caacgagctt gccgttgatc cgggctcgga agacatcccc ggcttttatg tggaggtcgg
     4201 acgccccgcg ctcgaccgca gccttgatga ttttttccat gtctgatcga ctcgttacca
     4261 gcagctattc agtcgttgac cggtgccacc gcagggagga accccatcag ctcgcgggcc
     4321 tgctgcagtg tctcccgcgc gattgcgcgc gcccgcatgg ctccggacgc caatatttcc
     4381 tcgacccgtg cagggtgggc ctggagctcg cgggcccgct cgcggatcgg cgcgaagtgc
     4441 gtcgtgatcg cgtcggcgag cacccgcttg caatcgagac agccccactt tgcaccccgg
     4501 cagttc
//
LOCUS       DASRJQ010000350         2702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3962529, whole genome shotgun sequence.
ACCESSION   DASRJQ010000350 DASRJQ010000000
VERSION     DASRJQ010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3962529"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VFB89_07725"
     CDS             complement(<1..230)
                     /locus_tag="VFB89_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27230.1"
                     /translation="MTDPDPRLTEEHAQKVLARALELQAQSPGTLTVTQIREIASELS
                     IPESAVDQALSEYRAAGADQLSTRTDAAGAPTP"
     gene            complement(324..1022)
                     /locus_tag="VFB89_07730"
     CDS             complement(324..1022)
                     /locus_tag="VFB89_07730"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZM27231.1"
                     /translation="MSVVLVIGGTRGTGRLIAHLLERQGITVRVLARDPERARTRLGP
                     TIEVIAGDITRTETLPRAIAGATHIVFTAGCRSGRPATETEIRSTEFGGVVNTLQAAR
                     EEGFRGRFLYMTSSGVTSRSLAAVMLNLYKGNTLVWRRRAEIEIRNSAVDYTIIRAGV
                     LLNQPGGQRAIVLTQDPLPLSIHYRIARADVAEAFVAALDHPRASHTTFDVVWGDGPR
                     GEPWPGLLARLKPD"
     gene            complement(1047..1571)
                     /locus_tag="VFB89_07735"
     CDS             complement(1047..1571)
                     /locus_tag="VFB89_07735"
                     /inference="COORDINATES: protein motif:HMM:NF023123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3291 domain-containing protein"
                     /protein_id="HZM27232.1"
                     /translation="MRRFHLAQVNIGRFRAPIDSPIMEGFRTQLDPVNALADRSPGFI
                     WRLQTEDGNATAVRPYPGDDLMAINLSVWETVEALQQFVYRSGHVHTLRARDNWFEPI
                     EGPILALWWIPAGHIPAVQEVVERLEHLKAHGPTPQAFTFRTPFPPPDGQAEVERLDA
                     EFCDWASHDMPRKA"
     gene            complement(1695..2357)
                     /locus_tag="VFB89_07740"
     CDS             complement(1695..2357)
                     /locus_tag="VFB89_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27233.1"
                     /translation="MPMPATPFHEYEDLALSPTYVLGYAFHGLNQLHARSPVHGRQGA
                     LGLLTELFATLTALKLEDSLRAAEPLRLCQETMASTRRSTRVGSAHAAEVRGTLTRIL
                     AAVQTDLSARAVPSARTRRLASRASASTGHLLTDAMRARCPESQRFDLMEGCRALASH
                     LPTASVFHLYRVWEALGSTAVAGLDVSMVADPRTDPTIRCEEADAIGVLSAIQRSLDD
                     SP"
BASE COUNT          488 a          855 c          878 g          481 t
ORIGIN      
        1 ggggtcggtg cacccgcggc atccgtgcgc gtggagagct ggtccgcacc ggcagctcga
       61 tactcagaga gcgcctggtc gaccgcagat tccgggatcg acagctctga ggcgatctca
      121 cgaatctggg tcaccgtgag cgtacccggg ctttgagcct gaagctccag cgcccgcgcc
      181 agcaccttct gcgcatgctc ttcagtgagt cgtggatccg gatcggtcat cggctgcctc
      241 ctcgccttgg catgagctgc ccggtaccgg ggctgcggaa actgccccag tccctcccca
      301 aacatacagc gtggcgggct ggatcaatcc ggtttcaggc gggccagcag cccaggccag
      361 ggctcaccgc gaggtccgtc gccccacacc acgtcaaacg tcgtatgaga agcgcgcggg
      421 tgatcgagcg cagccacgaa cgcctcggcc acgtctgctc gagcaattct gtagtgaatg
      481 gagagcggaa gcgggtcctg ggtgagcaca atcgcacgtt gtccgcccgg ctggttcagc
      541 aagacaccgg cgcggatgat ggtatagtcc acggcgctgt ttcgaatctc gatctcagcg
      601 cgccgacgcc ataccagcgt gttgcccttg tacagattga gcatcacggc ggcaagggac
      661 cggctcgtga caccactcga ggtcatgtag agaaaacgac cccggaagcc ctcctcgcgc
      721 gccgcttgca gagtgttgac gacaccgccg aattcggtgc tcctgatctc ggtttccgtg
      781 gcgggacggc cgctgcgaca gccggcagtg aatactatgt gtgtcgcgcc ggcaatcgcg
      841 cggggaagcg tctcggtccg agtgatatcg ccggcaatga cttcaatggt ggggccgagc
      901 ctggtcctgg cgcgctctgg atcccgcgcc agcacccgta ccgtgattcc ctggcgctcc
      961 aacaggtgag caatcaggcg cccggtcccg cgggtaccgc caatcaccag aacgaccgac
     1021 acataccccc tctggtcacc agggtgtcaa gcttttcgtg gcatgtcgtg tgacgcccag
     1081 tcgcagaatt ccgcgtccag gcgctcgacc tccgcctggc cgtcaggcgg agggaatgga
     1141 gttctgaagg tgaacgcctg aggggtcggt ccatgcgctt tcaggtgctc gaggcgctcc
     1201 accacttcct ggacagcagg aatgtggccg gccgggatcc accacagggc aaggatggga
     1261 ccctcgattg gctcgaacca gttgtctctg gcgcgcaggg tgtgtacgtg cccgctcctg
     1321 tagacgaatt gttgcagcgc ttccaccgtc tcccagacgg acaaattgat cgccatgaga
     1381 tcgtcgccag ggtagggccg gaccgcggtg gcgttgccat cttcggtctg caagcgccag
     1441 ataaatccgg ggctccggtc ggcgagcgca ttgacagggt caagctgggt ccgaaacccc
     1501 tccatgattg gcgagtcgat cggcgcccga aaccgtccga tgttcacttg cgccaagtgg
     1561 aagcgcctca tggctttcct cctcttgaac ccggacgttg cggcttaccg cgagctagtc
     1621 gcgcgatggg gacggggaat cccgggagcg ccagaggcac cgggatggtt ctggcatcgc
     1681 tcctaggctg tctactaggg ggaatcgtcg agcgatcttt gaatcgccga gaggacgccg
     1741 atggcgtcgg cctcctcaca gcggatggtt ggatcagttc tcggatcggc aaccatggaa
     1801 acatccaaac ccgcgactgc tgtgctacca agcgcttccc acacgcgata gaggtgaaac
     1861 acgctcgcag tgggcaggtg tgaggccagc gcacggcatc cttccatcaa atcaaagcgc
     1921 tgggactccg gacaacgcgc gcgcatcgca tcggtcagga gatggccggt gctcgcggag
     1981 gctcgcgacg ccaggcggcg agtgcgtgct gaagggaccg cgcgagcgct gaggtcggtt
     2041 tggacggccg caagaatgcg cgtaagcgtg cctctcacct cggcggcatg tgcagacccc
     2101 actcgggttg agcgccgggt gcttgccatt gtctcttggc acaagcgcag gggctcggcc
     2161 gcacgaaggc tatcctccag cttgagagcg gtaagtgtcg caaatagctc ggtcagaagg
     2221 cccagggcgc cttggcgtcc atgaaccggg cttcgggcgt gtagttggtt caggccatga
     2281 aaggcatagc ccaggacgta cgtggggctt agggccaggt cctcatactc gtgaaatggc
     2341 gtggcgggca taggcaccga gatcagttgt ccaactcacg acttgtgctt ccgttgcgag
     2401 gcctgccgaa gcccccgcgg gaccagactt aagatagcgc gcggccgctg tggcgcttag
     2461 gacccggcgg cagtcgcgcc gagcaggccc ctcaggaccc cacctgctcc tccaaggatg
     2521 ccggcgcaga tcagcggcaa accgtcgaca tcagacccct cgcaaccgac cacgcctacg
     2581 agggcccggg attccatcgg gagaacgcgt tgcccgctcg tgtctcactg gcttcagtgt
     2641 cgcgaactcg gtggatccgg ccgggcgtta tgggactctc ggtccctcga tcgtggctcc
     2701 ac
//
LOCUS       DASRJQ010000351         2132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3163497, whole genome shotgun sequence.
ACCESSION   DASRJQ010000351 DASRJQ010000000
VERSION     DASRJQ010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3163497"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            476..>2132
                     /locus_tag="VFB89_07745"
     CDS             476..>2132
                     /locus_tag="VFB89_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HZM27234.1"
                     /translation="MPLNKDGSSLPERVDHYRIKDKLGEGGMGVVYAAEDERLGRTVA
                     LKMLREATGDRSARERLRREARVAASISHPNVCRLYEVGEHQGELFLAMELLEGESLA
                     ARIVRGPLPLSDALPIMLSILDALDALHTQGVIHRDLKPSNVFLASVGIKLLDFGLAQ
                     RAETYVDRTETQLSLAGMLVGTPRYMAPERLQGRASDARADVFAAGAVLFELLIGKPV
                     FDGATIMEVMHAVIYEQTPVLGGSPAIAAADRIIHRAMAKNPDERYSSARAMADDIRS
                     ALQLTDSGEMIRARPMTRLIVLPFRILRSDPETDFLAFSLADAITISLSGLESLLVRS
                     TLTAARFGGDTPDLRALAGQADVDIVLTGTLLRAGDQLRVNAQLIEAPGGAVLWSLTS
                     QVPLGDVFQLQDALAQRIVGSLELPLSARDQRLLKHDVPATAKAYEFYLRANKLGYDP
                     KQWAVTRDLYLQCLEEDPSYAPAWARLGRICRVLGMYTERDSQELFRQAESAFKRALE
                     LNPQLPLAHNLYTYLEVELGGAREAMLRLLERAQGCAGDPELFA"
BASE COUNT          381 a          648 c          685 g          418 t
ORIGIN      
        1 cccggctgaa gtgttgagcg tgtagttgct catatcgacc ttctgcgtgg tgaaatccgg
       61 cgccgcctgg ccggctttga cctcgtttcc ggtgagagta acggggtttc ctttgaaagt
      121 tactgaagcc atggagatct ccttatgaaa agtatcgggg cgtcttgcac gttttcgatc
      181 ttgcgtccgt ggcagaggcc cgggtaaaag gtggccggaa atcatgccgc gccgtctcaa
      241 gccctgtcaa ggccgaaaac tggcgacatc gggcggtcct cgcgaggccc tggccgggcg
      301 cctctttgcg attcagagag cctgtcggga gcggcgaaag atcgagacgc aggacgttgc
      361 gaacgtccaa tttccaccct cacgataccg tctggaacct tcaatcggag ttgattccgt
      421 tctccggcgc tcccgagcgg ttggagaatc gggccacaac gagtattatc tccgcatgcc
      481 gctgaataag gatgggtctt ccttgcccga acgggtcgat cactaccgca tcaaggacaa
      541 actgggcgag ggtggcatgg gcgtggtcta tgccgccgag gacgagcgac tcgggcggac
      601 ggttgcgctc aagatgctac gagaggcgac gggtgacagg tcagctcgcg agcgattgcg
      661 gcgcgaggcc cgggtggccg ccagcatcag tcaccccaat gtgtgccggc tttacgaggt
      721 gggcgagcac caaggtgagc tgtttctggc tatggagctg ctggaagggg aatcgctggc
      781 agcccggatt gtcagaggtc cgcttccgct ttcggatgcg ctcccgatca tgctgtcgat
      841 cctggacgcg ctggacgcac ttcacactca gggcgtcatt caccgtgacc tcaagccctc
      901 caatgttttc ctggcatccg tcggaatcaa gcttctcgac ttcggactgg cgcagcgcgc
      961 cgagacttat gtggatcgca ccgagacgca gctgagtctg gcgggtatgc tggtgggaac
     1021 gccgcgatac atggctcccg aacggctcca gggacgcgcc agcgacgcgc gggcggacgt
     1081 cttcgccgcc ggagcggttc tgttcgagct gctgatcgga aagccggtat tcgatggcgc
     1141 gacgatcatg gaggtgatgc acgccgtaat ctacgagcag acgcctgtcc tgggcggatc
     1201 accagcgatc gccgccgccg accggatcat ccatcgggcc atggccaaga atccggatga
     1261 acgctatagc agcgcccggg ccatggccga cgacatccgg tccgcgttgc agctgaccga
     1321 ttccggagag atgattcggg cccggccgat gacccgcctg attgtgctcc cctttcgcat
     1381 tctccgctcc gacccggaga cggattttct ggctttcagc ctggccgacg ccatcaccat
     1441 ctccctttcc ggactcgaat cgctgctcgt gcgctccacc ctcacggccg ctcgtttcgg
     1501 aggcgacacg ccggatctcc gggcgctggc tgggcaggcc gatgtcgaca ttgtgctcac
     1561 cggcactctg ctgcgcgcgg gagaccagct gcgggtcaat gcgcagctga tagaagctcc
     1621 gggaggcgcc gtgctgtggt cgctcacatc acaggtaccg ctgggggacg tatttcagct
     1681 acaggacgca ctggcgcaac gcatcgtggg atcgctcgag ctgccgcttt cggcgcggga
     1741 tcagcgcctg ttgaagcatg atgtacccgc aacggccaag gcgtacgaat tctatcttcg
     1801 ggccaacaag ctcggatacg atcccaagca gtgggccgtc acccgcgatc tttaccttca
     1861 gtgcctggaa gaggacccga gctatgcgcc ggcatgggcg cggctggggc ggatttgccg
     1921 cgtgctggga atgtacaccg agcgagattc acaggagctc ttcaggcagg ccgagtcggc
     1981 attcaagcgc gcgctcgaat tgaatcctca gctgccgctg gctcacaacc tgtacaccta
     2041 cctcgaggtc gaactgggtg gcgctcgtga ggcgatgctg cgccttctcg aacgagccca
     2101 gggatgcgcc ggcgatcccg agctgtttgc cg
//
LOCUS       DASRJQ010000352         5622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_573259, whole genome shotgun sequence.
ACCESSION   DASRJQ010000352 DASRJQ010000000
VERSION     DASRJQ010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5622
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573259"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VFB89_07750"
     CDS             <1..862
                     /locus_tag="VFB89_07750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HZM27235.1"
                     /translation="LNRRAHVLRMREAVAWSLGLVAFALIFGLFILTREGGQAALEYY
                     TGYLIELSLSVDNLFVFILIFSYFHVPAEAQPKVLKWGILGAIGMRFVMIAVGGLLLQ
                     RFEWITWVFGGILVFTGIKMWQQKELHIEPERNPVVRLARRLLPFSSNYEKTDFFTLS
                     RDGRRLATPLFLVLLVVEWSDLVFAIDSIPAVFAVTRDPFLVFSSNVFAILGLRALFF
                     VLSGLLDKFVYLRPGVAGILVFVGLKMALSNWIHIPTGVSLGVIITTLAGAVGLSLWR
                     RNTTQQRIAT"
     gene            complement(935..2098)
                     /locus_tag="VFB89_07755"
     CDS             complement(935..2098)
                     /locus_tag="VFB89_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HZM27236.1"
                     /translation="MISYQLSAIGYQLALLLGTAAAAPPPPERTYFVYVASEGVDRIQ
                     LVRFGPGGASVERSFRIGLVPTEPSGPHGLAVAPGGKSFFVSTGHGAPFGSLWKFSSA
                     DDKPQGRVELGLFPATLQVSPDGYLAYVVNFNLYGDMVPSSVSVVATAEMVEVARIPT
                     CIMPHGSRLNPQGTRHYSACMMDDMLVEIDTRKLAVSRHFFLRKGQERGMEGPVVAST
                     TEANTSRAGHDMGGHGMTAPKPGDVTCSPTWAQPSADGSRIYVACNKASEIVEIDATS
                     WTISRRWPAGEGVYNLAATHDGKLLIATNKRGQSVSIFGLAAGRELKRLPTKRKVVHG
                     VAVSPDDRYAFVSVEGIGSQPGTVEMIDLRALQTVATVDVGSMAGGIDVWKEE"
     gene            complement(2095..4107)
                     /locus_tag="VFB89_07760"
     CDS             complement(2095..4107)
                     /locus_tag="VFB89_07760"
                     /inference="COORDINATES: protein motif:HMM:NF014427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZM27237.1"
                     /translation="MKVLALIMAGALASAGPLASQQATVARLVVTPTHPEVMAGDSLR
                     LTAQALDANGAPVPDAKITFKKEGLQGADVDDEGWFRAGARGTMQVVVSAIVPGAKPV
                     IDQVTVRMLPGPAAKVEIGDHPGRLVPGQRYQLTARSLSAGGDLRDDQIVWTSSAPSV
                     AHVTRDGMLEGRAAGRATLTAAVGSVRATTPVAVKSAMPVSLTIEPAAPKVRQGDVVR
                     FVAVAKDKNGKTISGLTPSWSLSPGRGEVYADGGFVGYDPGVYTVTATLGNRSAVAAV
                     QVTPRDVRRPATVVGKSIRKAFPTSEVWVHPNGKVLYLGTHLGGDRFYSIDITDPANP
                     VIVDSVQANTRLINDIMTDKEGKVLVFTREGAADRKNGLVICTLDDPLHPKVVSEFTD
                     GVTAGVHSSFVYTQPKYGTHVYLTNDGTGALHILDINDPAHPREVGRWKTPRADAGRV
                     LHDVDVQDGLVYASWWDDGLVILDVGNGVKGGSPSNPQLVSQFKYDLDKLYKQVAETS
                     GPGFIRGTHTAWRHGQYVFVADEVFGLESAGDLFEGRPSRAYGRLHVVDVTDLAHPKE
                     VAWYEPEYGGVHNVWVAGDTLYMGAYNAGFRAFDISGELRGDLRAQDREIAQVNTIDK
                     DGFIPNAAMTWGVVVKDGLAYVNDFNNGLFVVRLEPRRDQSQPMLP"
     gene            4354..4746
                     /locus_tag="VFB89_07765"
     CDS             4354..4746
                     /locus_tag="VFB89_07765"
                     /inference="COORDINATES: protein motif:HMM:NF012999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HZM27238.1"
                     /translation="MRSVHGLVLVFGAIGVGAGCGSGEENGPSATGPTASFSSSCINL
                     ECSFSNLSRDGDGAIATYRWDFGDGAAAATKNAQHTYAAASTYAVRLTVTDNAGISGE
                     VTHAMQLNRLSNMPPTGVPLAPPTSVGR"
     gene            4809..>5622
                     /locus_tag="VFB89_07770"
     CDS             4809..>5622
                     /locus_tag="VFB89_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZM27239.1"
                     /translation="MRISARERRGFSGGLASCGAPFRGYRGFATLHAPMPPSLLHRLG
                     LHRPELRAWAMYDWAISAVQVGIVTAIFPIYYVKVAGAGLVEGKATQHLATANTVSLA
                     FVALLSPILGAAADYVAAKKRFLAAFMLLGLLAIAAMFFIHTGDLLLASVLFVLAMIG
                     ATGSFVFYEALLPHIASPEEVDRVSTAGYALGYIGGGVLLAVNLAWIQKPAWFGLPSG
                     PGLAEAAATLPVRLAFVSIAAWWVLFSIPLFRRVPEPPRRLEQDERRGENPVV"
BASE COUNT          943 a         1846 c         1741 g         1092 t
ORIGIN      
        1 gctcaaccgc agggctcatg tgctgcgcat gcgagaggcg gtagcgtgga gcctgggtct
       61 cgtggctttc gcgctcattt ttggcctgtt catcctcact cgcgagggtg ggcaggcagc
      121 gctggagtat tacacggggt atctgatcga gctctccctg agcgtcgaca acctgttcgt
      181 cttcattctg attttctcgt acttccacgt cccagcagaa gcccagccca aggttctcaa
      241 atggggaatt ctaggcgcga tcggcatgcg gttcgtgatg atcgcagttg gtggactgct
      301 attgcagaga ttcgagtgga tcacctgggt gttcggcggc atcctggtgt tcacgggcat
      361 caagatgtgg cagcagaaag agctgcacat tgagcccgag cggaatcccg tcgtgcggct
      421 ggcccgccgc ctgctgcctt tctcgtcgaa ttacgagaaa accgatttct tcaccttgag
      481 ccgcgacggt cgacggctcg caaccccgtt atttctggtg ttgctcgtgg tcgagtggtc
      541 ggatctggta tttgcgatcg acagtatccc tgccgttttt gccgtcaccc gggatccgtt
      601 tctggtcttc agctcgaatg tgtttgcgat tcttggccta cgagcactct tcttcgtgct
      661 gtcgggcttg ctcgataagt tcgtctatct aagaccagga gtggcgggca tcctcgtatt
      721 cgtaggcctc aagatggcgt tgagcaactg gattcacata ccgaccgggg tgtcgctagg
      781 ggtgatcatc actacgctgg ctggcgcggt cggcctttcg ttgtggcggc gcaataccac
      841 tcagcagcgc atcgccacct gatccctctc taagccggag tcgcaagccc acggcggcgc
      901 cagcgctcgc ctttgaagat cgaagcgaac tccactactc ctccttccag acgtcaatcc
      961 cgcccgccat agacccgaca tccaccgtcg ccactgtctg aagagcacga aggtctatca
     1021 tctcgaccgt gcccggctgc gagccgattc cttcgaccga cacgaacgcg taccgatcgt
     1081 ccggagacac ggcgacgccg tgaaccacct tgcgtttggt cggcaggcgc ttgagctcac
     1141 gccctgcggc gaggccgaag atcgagaccg actggccccg cttgttcgtc gcgatcagga
     1201 gtttgccgtc gtgcgtagca gcgaggttgt aaacgccctc gccggccggc cagcgccgac
     1261 tgatggtcca gctcgtggcg tctatctcga cgatctcgct cgccttgttg caggccacat
     1321 agatcctgct cccgtccgct gacggttgag cccaggttgg cgagcaggtc acgtcgccag
     1381 gcttcggggc cgtcatgcca tgcccgccca tgtcatgccc tgcccgactc gtgttcgctt
     1441 ccgtcgtcga ggcgacaacc ggcccctcca ttccgcgctc ctgtcctttc cggagaaaga
     1501 agtggcggga gaccgcgagc ttccgggtat cgatttcaac cagcatgtcg tccatcatgc
     1561 aggccgaata gtgcctggtc ccctgcgggt tgaggcggga gccgtgaggc atgatacagg
     1621 tggggattcg agcgacctcc accatctcgg cggtggcgac caccgatacg gacgaaggca
     1681 ccatgtcgcc gtagagattg aaattcacga cgtaggcgag atacccgtcg ggcgacacct
     1741 gcagcgttgc gggaaagagc ccaagctcta ccctgccctg tggcttgtca tcagcgctgg
     1801 agaacttcca gagcgagccg aacggcgcgc cgtgaccagt cgatacgaag aaggatttgc
     1861 cgccaggcgc cacggcgagt ccgtggggcc cggagggctc ggtcggcact aggccgatcc
     1921 gaaagctgcg ttcgaccgat gcccctcccg gaccgaagcg caccagctga atccgatcta
     1981 cgccttctga cgcgacataa acgaagtagg tacgctccgg cgggggcgga gccgcggcag
     2041 ccgtgccgag cagcagggcc agctgatacc cgatagctga tagctgatag ctgatcacgg
     2101 cagcatcggt tggctctgat cgcgccgcgg ctcgagccgc accacgaaaa gcccgttgtt
     2161 gaagtcgttc acgtacgcga gaccgtcctt caccaccacg ccccaggtca tcgcggcgtt
     2221 gggaatgaag ccgtccttgt cgatcgtatt cacctgcgcg atctcccggt cctgggcgcg
     2281 gagatctccc ctcagctcgc cggagatatc gaaggcccgg aatccggcgt tgtacgctcc
     2341 catgtacagc gtgtcgccgg caacccagac attgtgcacg ccgccgtact ccggctcgta
     2401 ccaggcgacc tctttgggat gcgccagatc ggttacgtcc acgacatgca accgcccgta
     2461 cgcgcgcgag ggtcggccct cgaacaggtc tcccgcggac tcgagcccga agacttcgtc
     2521 tgccacgaag acgtactggc catgccgcca ggcggtatgg gtcccgcgaa tgaaccccgg
     2581 cccgctcgtc tcagccacct gcttgtacag cttgtcgagg tcgtacttga actgggaaac
     2641 gagctgggga ttcgatgggc tgccaccctt gactccgttt ccaacatcca ggatcacgag
     2701 cccatcgtcc caccagctgg catacaccag tccatcctgt acgtccacat cgtgcagcac
     2761 ccgccccgcg tccgccctcg gcgtcttcca gcgcccgact tccctgggat gagccggatc
     2821 gttgatgtcg aggatgtgga gcgctcccgt gccgtcgttg gtgaggtaga cgtgcgtacc
     2881 gtacttcggc tgagtgtaaa cgaacgaaga atgaacgccg gccgtgacgc cgtcggtaaa
     2941 ctcggagacg accttggggt gtagcgggtc gtccagcgtg cagatcacca gcccgttctt
     3001 tcgatcggcc gcgccctcgc gggtgaagac gaggactttc ccctccttgt cggtcatgat
     3061 gtcgttgatc agacgggtat tggcctggac cgaatcgacg atgaccgggt tggcaggatc
     3121 ggtgatgtcg atgctgtaaa agcgatcgcc gcccaggtga gtgccgaggt agagcacctt
     3181 cccgttggga tgaacccaga cctccgatgt cggaaacgcc tttcgaatcg actttccgac
     3241 caccgtggcc gggcggcgca cgtcgcgcgg ggtcacctgg accgccgcga ccgccgaacg
     3301 gttcccgagc gtggccgtga cggtgtagac gcccgggtca taaccgacga acccgccgtc
     3361 cgcatagacc tctccgcggc ccgggctgag tgaccagctc ggcgtgaggc ccgagattgt
     3421 cttgccgttc ttgtccttcg ccacagcgac aaaccgcact acatcgccct gtcgcacctt
     3481 gggcgcagcc ggctcgattg tcagcgacac gggcatcgca gatttcaccg cgactggcgt
     3541 cgtcgcgcgg acgctgccga ccgctgcggt gagcgtcgcc cggccggctg cgcgtccctc
     3601 gagcatcccg tcccgggtca cgtgagccac cgatggcgcc gagctggtcc aaacgatctg
     3661 gtcgtcccgc agatcgccgc cggcgctgag cgagcgcgcc gtcagctgat atcgctggcc
     3721 cgggaccagg cgcccggggt gatctccgat ctctaccttc gcggcaggcc ccggcagcat
     3781 gcgcaccgtg acttgatcga tgaccggctt cgctcccggc acgatcgccg agaccactac
     3841 ctgcatcgtg ccgcgtgcgc cggcacggaa ccaaccctcg tcgtcaacgt cggcgccctg
     3901 aagtccctct ttcttgaagg tgatcttggc atcgggcacg ggcgcgccgt tcgcatcgag
     3961 ggcctgcgcc gtcagtcgaa gcgaatcgcc ggccatgacc tccggatgcg tcggcgtcac
     4021 taccagccgg gcgaccgtcg cctgttgcga ggcaagcggg cccgcggagg cgagagcccc
     4081 tgccataatc agcgccagga ccttcatgtc cacggttcct tgttcgggcg gttccacggg
     4141 taacgagcta tccaggacat tcaagctgcc gtgccgcacc ggtgatgtca aagcgccatt
     4201 cgctcccgtg cttgactctc cgcctccctg aattgtccgc caattgttac caaccgcgta
     4261 tcctcgtcgc caacccggca gacaccgttg agggcccgtc tcactctggg gcggggctgt
     4321 cgggggacca tgtcagagcg ggagcggtgc accatgcgat cagttcacgg cctcgtcctg
     4381 gttttcggcg cgatcggagt cggcgccggc tgtggcagcg gagaagaaaa cggaccatcg
     4441 gccacggggc cgaccgcgag cttcagcagc tcgtgcatca acctggaatg cagtttcagt
     4501 aacctgagca gggacggcga cggagcaatc gctacctaca ggtgggattt cggcgacggg
     4561 gccgctgctg cgacgaagaa tgcccagcac acgtacgcgg ccgcgagcac gtatgccgtc
     4621 agattgaccg tcacggacaa cgctggtatt tccggcgagg ttacccacgc catgcaactc
     4681 aaccggctat ccaacatgcc accgaccggt gtcccgttgg cgcccccaac gagcgtgggc
     4741 cgctgactgg agggcacgca agacaggagg tctaccgccg cggggtgacg ggtgctcatc
     4801 acttgaccgt gcgcatcagc gcccgggaac ggcggggctt cagcggcggc ctcgcctcct
     4861 gcggtgcgcc ctttcgcgga taccggggct tcgccaccct tcacgcaccc atgcccccgt
     4921 ccctcctcca ccgacttggc ctgcatcgtc ccgagctacg tgcctgggcc atgtacgact
     4981 gggcgatctc ggccgtgcag gtcggcatcg tgacggcgat cttcccgatc tactacgtga
     5041 aggtcgccgg ggcgggattg gtcgagggaa aggcgaccca gcacctcgcg acggctaaca
     5101 cagtctcgct cgcattcgtc gcacttctgt ctcctattct cggtgcagcc gcggattatg
     5161 tcgcagcgaa gaagcgattc ctcgcggcgt tcatgctcct cggcctcctg gcaatcgcgg
     5221 cgatgttttt catccacacc ggagacttac tgctcgcatc ggtcctgttc gtgctcgcga
     5281 tgatcggcgc caccgggagc ttcgtgttct acgaggcgct gcttccgcac attgcctcgc
     5341 cggaagaggt ggatcgcgtc tcgacggcgg gatacgctct cggctacatc ggcgggggtg
     5401 tgctgctggc ggtcaatctt gcctggattc agaagccagc ctggttcggt ctgccctccg
     5461 gccccgggct cgcggaagcc gccgccacgc tgccggtgcg gttggcgttc gtgtccatag
     5521 ccgcctggtg ggtgctgttc tcgattcctc tctttcggcg ggtgccggag cctcctcgcc
     5581 ggctcgagca ggacgagcgg cggggggaaa atccagttgt ga
//
LOCUS       DASRJQ010000353         6622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1427174, whole genome shotgun sequence.
ACCESSION   DASRJQ010000353 DASRJQ010000000
VERSION     DASRJQ010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6622
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1427174"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VFB89_07775"
     CDS             complement(<1..405)
                     /locus_tag="VFB89_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27240.1"
                     /translation="MQLPTDSLRAVLDSVFAEPAYAWVDEPQPLAFVRDWWGRLADWI
                     TATRSLHPLLFQLLVFALAAVLVAILVHAGYVFQQTIKAAAGRESKTMLQSAQVRRDA
                     AWYLAAADQAAAAGRYGDALRLAFEALILRLDA"
     gene            complement(431..1201)
                     /locus_tag="VFB89_07780"
     CDS             complement(431..1201)
                     /locus_tag="VFB89_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27241.1"
                     /translation="MASLALRPLTIGEVIDASFQVYRRHFSALATIVLVCNGLPALLQ
                     IYGRTGPGIQLMLTLVYMVLVVVLSLVATGATVFVVSESYLGRSITAREALNRALPFV
                     VPLIIVSLLVGLVAFVGFLLIVVPGLIAVCGLAVTVPALVVESLAPTDAMGRSWALTR
                     GYRLRMFGLFVLIFLLILIPYIAIMGMAGVAGAVFGATGAKGVSTTIILLGTVLAAIV
                     QLLLSPLYQAALVIAYYDLRVRKEGFDLEVLASVLQPV"
     gene            1288..3111
                     /locus_tag="VFB89_07785"
     CDS             1288..3111
                     /locus_tag="VFB89_07785"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HZM27242.1"
                     /translation="MATRAPFDLRQHLEVETPEHVVLDLEIAGVGSRLLAAIIDSAIV
                     TAIMMTAGLLAGTLSSVGLLPSSRYGGAWLLAIIIGLVFALGNGYFILFEGLRGGQTP
                     GKRWVGIRVVRETGHPITLGAAVLRNLLRVADFLPPPYLLGLALIAFHPRAKRLGDLV
                     AGTVVVRDRPSPVSSFRIAQSTTTGSPGDWSQSSIELDDEQFRLLARYASRAADLPPE
                     ARERIAASLERRFPLQRPPTSSPADFLLDLHARELARRRGRLGGRRGVGDRLAARQTD
                     RWDAFQRLAERATSQGLDSFAPAELPDFAARYRELAADLARARTYHADGAIIARLERL
                     VSAGHNVLYRDDPHTWTRVWTAISRECPAAVVQTRWLVLLAWALFTAPALGGYALMRE
                     RPELANQVLPEIMLRRAEAGKERAASGQKYGSVEATGRPVIASFIITNNIRVAIACFA
                     GGVFFGVGSLALLAFNGLAIGTFAGHFANQGLLGYLMEFVVGHGVLELFSIWVAGAAG
                     FLLGKSLLVPGDRSRADAAVVSGRVAVRMMGAVVVLLVIAGTIEGFLSVSGRGLAARL
                     AASVASLTFLAAYLARGVAWRPRLELPEHGAHSDRERLDLA"
     gene            complement(3055..3237)
                     /locus_tag="VFB89_07790"
     CDS             complement(3055..3237)
                     /locus_tag="VFB89_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27243.1"
                     /translation="MTEAERLSKLRHDLSNPLSALLAEIQLLLLEEQRYDPETLSGLR
                     EIESLAIRMRTMLREL"
     gene            complement(3247..3531)
                     /locus_tag="VFB89_07795"
     CDS             complement(3247..3531)
                     /locus_tag="VFB89_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27244.1"
                     /translation="MALQTVRPPTRDPREPVQHELDALRFNGQVVLLSLIVRHAADGA
                     WRARLRFLDSDSGRERETVEIFCAESEADLWESVRELRGHHIRDLYRSLD"
     gene            complement(3702..4856)
                     /locus_tag="VFB89_07800"
     CDS             complement(3702..4856)
                     /locus_tag="VFB89_07800"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZM27245.1"
                     /translation="MQEASRGAIDSLTATFEAWRSHAIGRREYTEALVSPAHRGPSPA
                     LAGALAAWPGRYYWADVDRSHLVLIRSIGTKRGERWLLHVILFAVTVICALGAGATLA
                     GTWTPTEPPGLVGVLIGVSRFFERLAGPGWRDLVAGWSFAVPLLAILLVHELGHYFAA
                     RRYGIDASPPFFLPVPPNLSPIGSLGAYIRLRSPVLDRRQLLDVGAAGPLAGFALTLL
                     VLVWGFSTSEPIEPGLHPIGSFVRLAGERIELGDSLLMIACRKLLLPGAQTVQLSMPA
                     FAGWVGAFVTGLNLLPLSQLDGGHVVYGLLGRRQSAVGLITLLVLIGLAQLSWNWYVW
                     VAVALFVGGGGWAHPSVLAPARPVPPSRRWIGWLSMVVFVLTFVPVPFGR"
     gene            5031..6278
                     /gene="rho"
                     /locus_tag="VFB89_07805"
     CDS             5031..6278
                     /gene="rho"
                     /locus_tag="VFB89_07805"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HZM27246.1"
                     /translation="MDIAELKQKSVAELHQLAEQLNISNYSGLRKQDLIFRIEQSLLD
                     QDTVIRGEGVLEILPEGYGFLRSQDWNYLYGPDDIYVSPSQIKRFDLRTGDTVMGQVR
                     PPKEGERYLALLKVENVNFEEPEKTKHRIAFDNLRPRYPESRIRLERKNGDLSMRVVD
                     LLAPLGKGQRALIVSPPKAGKTILMQKLANAISENHPEIVLIVLLIDERPEEVTDMEE
                     NVKAEVISSTFDEPADRHVQVADMVIEKSKRLVEHGRDVVILLDSITRLARAHNVVVP
                     HSGKILSGGVDANALQKPKRFFGAARNIDKGGSLSIIATALIDTGSRMDEVIFEEFKG
                     TGNSELVLDRRLADRRIYPAIDIQRTSTRKEELLMDKDELNRVYLLRNFLADMPPVEA
                     LEFLLERMKRTKNNKEFFATMAQ"
     gene            6370..>6622
                     /locus_tag="VFB89_07810"
     CDS             6370..>6622
                     /locus_tag="VFB89_07810"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RuvX/YqgF family protein"
                     /protein_id="HZM27247.1"
                     /translation="MSIPPHGRILALDWGVVRIGLALSDESQTLASPLETLVRRRGKR
                     LPMPRLLQLIEEHRPVAILVGLPLTGEGEEGESALAARKT"
BASE COUNT         1303 a         2118 c         2097 g         1104 t
ORIGIN      
        1 ggcatcgagt cgaaggatga gtgcctcgaa ggccagccga agcgcatcgc cgtagcgccc
       61 ggctgcggcg gcctggtcgg ctgccgcgag ataccaggct gcgtcccgcc gcacctgcgc
      121 cgattgcagc atggttttcg actcgcgacc tgcggccgcc ttgatggtct gctgaaagac
      181 gtatccggca tgaaccagaa tcgccaccag aacagcggcg agcgcgaaga cgaggagttg
      241 gaagagcagc gggtgtagcg atcgcgtagc agtgatccaa tctgccagcc gtccccacca
      301 gtcacgcacg aacgccagcg gctgcggctc atcgacccag gcgtaggccg gttcagcaaa
      361 aaccgagtcc agaaccgctc gcagcgagtc tgtgggaagc tgcatcgtcc ggggcgagct
      421 gacccttcag tcagacgggt tgaaggacgg aggcgaggac ctcgagatcg aagccctcct
      481 tacgaacccg aagatcgtag tacgcgatga ccagcgccgc ctggtacaag ggcgagagga
      541 gaagctgcac gatcgccgcc aggaccgtcc ccagaaggat gatggtcgtg ctgaccccct
      601 ttgccccggt cgcgccgaac acggcgcccg caactccggc cattcccatg atggcgatat
      661 atggaatgag gatcagcaga aagatgagca cgaacagccc gaacatccgc agtcggtacc
      721 cgcgagtgag ggcccaggac cgccccatag catcggtcgg tgccagagac tccactacca
      781 gcgccggaac cgtgacagcg agcccacaca cggcgatcaa gccagggaca acgattagca
      841 ggaaccccac aaatgccacc aacccaacca gcaacgatac gatgatcagt ggaacgacga
      901 agggcagcgc ccggttgagc gcctcccgcg ccgtgatcga gcggcccaga tagctctcgg
      961 agaccacgaa cacggtagcc cccgtggcga ccaggctcag aaccacgacc agaaccatgt
     1021 acaccagcgt gagcatcagc tgaattccgg ggccagtccg accgtagatc tggagcagcg
     1081 ccggcagtcc gttgcacacg aggacgatcg tcgcgagcgc actgaaatga cgccgataca
     1141 cctggaaaga ggcatcgatg acctcgccga tagtcaatgg gcgcagcgca agggaagcca
     1201 tgaagactcc tcgtgagacg ggcaagcacc tggcgggggt cggaggaagt taattaccta
     1261 tcaggttcca ccaacggttc acgcttcgtg gcgaccagag ccccgttcga tcttcgtcag
     1321 cacctcgaag tcgagacgcc cgagcacgtc gtgctcgacc tggagattgc cggggtcggt
     1381 tcgcggctgc tcgccgccat catcgacagc gcgatcgtca cagccatcat gatgaccgct
     1441 ggccttctcg ccgggacgct ttccagcgtc ggcttgttac cgtcttcccg ctacgggggc
     1501 gcctggctct tggcgatcat cattgggttg gtgtttgcgc tgggcaacgg atacttcatt
     1561 ctgttcgagg gcctgcgcgg cggccagaca ccgggaaaac gctgggtcgg cattcgagtc
     1621 gtacgggaaa cgggacaccc gattaccctg ggagccgccg tccttcgcaa tctcttgcgg
     1681 gtcgccgatt ttctgccccc gccctacctg ctcggcctgg ccctcattgc tttccatccc
     1741 cgcgccaagc gactaggcga cctggtggcg ggcaccgtcg tcgtgcgcga ccgccccagt
     1801 cctgtctcga gcttccggat cgctcagagt acaacaaccg gctctcccgg agactggtcg
     1861 cagagcagta tcgaattgga cgacgaacag tttcggctac tggcccgcta tgcgagccga
     1921 gcggccgatc tgccgccgga ggcgcgggag cggattgccg ccagcctgga aagacggttc
     1981 cctctccagc ggcctccgac ctcgagcccg gctgattttc tgctggacct gcacgctcgg
     2041 gaactcgctc ggcgccgggg aaggctcggc ggtcgtcgag gtgtcgggga tcgcctcgca
     2101 gcccgccaga ccgaccgctg ggacgcattt caacggctgg cagagcgcgc cacgtcccag
     2161 gggctcgaca gcttcgctcc cgccgagctc cccgacttcg cggcgcgcta tcgcgagctg
     2221 gctgccgatc tcgctcgggc caggacctac catgccgacg gcgctatcat cgcgcggctc
     2281 gaacgcctgg tgtccgcggg tcataacgtg ctctaccggg atgacccgca cacgtggacc
     2341 cgggtgtgga cggcgatcag ccgggagtgc cccgcggcgg tcgtgcagac tcgctggctg
     2401 gtgcttcttg cctgggcctt gtttacggcg ccggcactgg gcggttatgc cctcatgcgc
     2461 gagcgacccg agctggcgaa tcaagttctt cccgagatca tgctacgacg tgcagaggca
     2521 ggaaaagaac gggccgcatc ggggcagaag tatggatccg tcgaggcgac cgggcggccc
     2581 gtgatcgcct cgttcatcat taccaacaac atccgcgtgg cgattgcgtg cttcgccggg
     2641 ggcgtgtttt tcggcgtcgg gtctcttgct ttgctcgcgt tcaacgggct tgcgatcggc
     2701 accttcgccg gccatttcgc caaccaaggg ctgctcggat acctgatgga gttcgtcgtg
     2761 gggcacggcg ttctcgaact tttttcgatc tgggttgcgg gagccgccgg ctttttgctg
     2821 ggaaagtcgc tgctcgttcc cggcgatcgg tcccgggccg acgcagcggt cgtgagcggc
     2881 cgcgtggcgg tcagaatgat gggcgctgtc gtggtcctgc tggtgatcgc ggggacgatc
     2941 gagggatttc tctcggtgag cggccgcggc ctcgctgcgc gcctggccgc gagcgtagcg
     3001 agcctcacct ttctcgcagc gtacctcgct cgcggcgtgg cctggcgacc tcggctagag
     3061 ctcccggagc atggtgcgca ttcggatcgc gagcgactcg atctcgcgta gacccgacaa
     3121 ggtctcggga tcgtatcgct gctcctcgag cagcagaagc tggatctcag ccagcagagc
     3181 ggacaatgga ttggacagat cgtgccgcaa cttgctgagc cgctccgcct ctgtcatcct
     3241 ccgaactcag tcgagcgacc gatagaggtc gcggatgtga tggccgcgga gctcccgcac
     3301 cgactcccag aggtcagctt ccgattcggc gcagaagatt tcgacagttt cgcgctcgcg
     3361 accggaatcc gagtcgagga agcggagccg ggcccgccag gctccatccg cagcgtgccg
     3421 aacgatgagg ctgagcagaa cgacttgacc gttgaagcga agggcatcga gctcgtgttg
     3481 aacgggctcc cgggggtccc gggtcggggg gcgcactgtt tgcagggcca tcagggcagg
     3541 tcccggcagg tttggctaaa aatacatggc gggcggagtt agacaatagc tcttttagcc
     3601 ttcagctatc agtctctgct aaccggcacc cgcaatcccc gaggtgctga ccgtcgcagt
     3661 tctgtcacgc atcggcgccc cagccttctg atagctgata gctaccttcc aaatggcacc
     3721 ggcacgaacg tgagcacgaa caccaccatg gacaaccaac cgatccaccg ccgactcgga
     3781 ggcaccggtc gcgcaggcgc gagcacggat ggatgcgccc aacctccgcc gccaacgaac
     3841 agagccactg ccacccacac gtaccaattc cacgacagct gagcgagccc gatcagaacg
     3901 agcagtgtga tgagacccac agccgattgg cgccgtccca gaagaccata gacgacgtgg
     3961 ccgccatcga gctgactgag aggaagcagg ttgagtccgg tgacgaacgc accgacccac
     4021 cctgcaaagg cgggcatgct gagctggacc gtctgcgcgc cgggaagcag gagtttccgg
     4081 caggcgatca tcaggagaga gtcacccagt tcgatgcgct ccccggccag ccgcacgaag
     4141 ctgccgattg gatgaagccc cggctctatc ggctcggatg tgctgaaccc ccacaccaac
     4201 acgaggagtg tcagggcgaa gccggccagc ggacccgcgg cgccgacgtc gagcagctgc
     4261 cggcggtcga gcaccggcga gcggagcctg atataggctc ccaagctgcc gatgggtgaa
     4321 aggttgggcg gcacgggcag gaaaaacgga ggcgacgcat cgatcccgta acgccgggcg
     4381 gcgaaatagt gccccagttc gtgaaccaga agaatggcca gcagaggcac cgcaaacgac
     4441 catccggcga cgagatctcg ccagcctggt ccggccagtc gttcaaagaa gcgactgacg
     4501 ccgatgagga ctcccacaag accgggcggt tcagtcggcg tccaggtccc cgccaaggtt
     4561 gcgccggcac cgagcgcgca gatcacggtc accgcgaaca ggatgacgtg gagcagccac
     4621 cgctctcccc ttttggtgcc gatggaacgg atcaggacga gatgagagcg gtcgacatcg
     4681 gcccagtaat agcggccggg ccacgcggcg agggcgccgg ccagggctgg cgatggcccc
     4741 cggtgggccg gtgaaaccag ggcctcggtg tattcccttc gtccgatggc gtggctgcgc
     4801 cacgcctcga aggtggcggt aagggaatct atggcgcctc ttgacgcttc ttgcaacgag
     4861 gtctaagttg agttgcttct gcggccgatt accatctccc cagaccatct cgattcacac
     4921 gcttcgccgc ggcggagctc gtcacctaca ccgatactgt cctttgcatt gcctcattcc
     4981 gtcgagcccg tcggtccgcc gatcgggcac ccctgcgtgg agtcccgtcc gtggatatcg
     5041 ctgagctaaa gcagaaatcc gtcgcggagc tacatcagct ggccgagcaa ctgaacatct
     5101 cgaactactc gggcctccgc aagcaagatc tcatcttccg tatcgagcaa tcgctgctgg
     5161 accaggacac ggtcatccgt ggcgagggtg tgctcgagat tctccctgag ggctacgggt
     5221 ttctccggag tcaggactgg aactacctgt acggccctga tgatatctac gtctccccca
     5281 gtcagatcaa gcgcttcgac ctccgcaccg gtgacacggt catgggtcag gtgcgcccgc
     5341 ccaaggaggg cgagcgctat ctggcgctgc tcaaggtcga gaacgtcaac tttgaggagc
     5401 cggaaaagac caagcaccgg atcgccttcg acaacctgcg gccccggtac cccgagtccc
     5461 ggatccgctt ggagcggaaa aacggcgacc tatcgatgcg ggtggtcgat ctgctcgctc
     5521 cattgggcaa ggggcagcgc gcgctgatcg tgtccccgcc caaggctggc aagacgattc
     5581 tcatgcaaaa gctggccaac gcgatcagcg agaatcaccc ggagatcgtg ctgatcgtgc
     5641 tgctcatcga tgagcgaccc gaagaagtga ccgacatgga agagaacgtg aaggccgagg
     5701 tcatttcttc gaccttcgat gagccggccg accgccacgt ccaggtcgca gacatggtga
     5761 tcgagaaatc aaagcgattg gtggagcacg ggcgcgacgt ggtcattctg ctcgactcga
     5821 tcacccgtct agcgcgggcc cacaatgtcg tggtaccgca ctccggtaag atcctttctg
     5881 gcggcgtcga tgccaacgca ctccagaagc cgaagcggtt cttcggcgcc gcccgcaaca
     5941 tcgataaggg cggcagcctg agcatcatcg cgaccgcgct gatcgatacc ggcagccgga
     6001 tggacgaagt catcttcgag gagttcaagg gcaccggcaa ctcggagctg gtgctcgatc
     6061 ggcgcctggc tgatcggcgg atctatcctg ccatcgacat ccagcgaacg tcgacgcgga
     6121 aggaagagct gctcatggac aaggacgagc tcaaccgagt ctacctgctg cggaacttcc
     6181 tggccgacat gccgccggtt gaagcgctgg aattcctgct cgagcggatg aaaagaacca
     6241 agaacaacaa ggaattcttc gcgacgatgg cgcaataaaa agactgcgtg agcagtgaac
     6301 agtgaacggg ggactccgag tacatcgaag ccccccgttc accgtttacc gtttaccgtt
     6361 cacgctgtca tgtcgatccc accccacggc cgcattcttg ctctggactg gggcgtagtc
     6421 cgcatcggac tcgccctgag cgatgagagc cagacactcg cgtcaccgct cgagaccctg
     6481 gtacggcgaa gggggaagcg gttgcccatg cctcggctac tgcagctgat cgaggaacac
     6541 agacccgtcg cgattctggt tggcctcccc ctcaccggcg agggcgagga aggcgagagc
     6601 gccctggccg cccggaaaac cg
//
LOCUS       DASRJQ010000354         3260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3717595, whole genome shotgun sequence.
ACCESSION   DASRJQ010000354 DASRJQ010000000
VERSION     DASRJQ010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3717595"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..790
                     /locus_tag="VFB89_07815"
     CDS             143..790
                     /locus_tag="VFB89_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27248.1"
                     /translation="MVWNRAIRRARAQWALAVCGTLALSACGSKYALVPPRLDLEPYG
                     RVALVTFSGDQTNNELNTLATQRFAEAVLASQTGFELLELGAADSSLKRIAATGDGEA
                     LAHAVGKVKNVPAVFVGHLSVSGVKPHGRLSGVSSLKVRASVSAQLSVQLLSTSTGGT
                     VWRSSATADGTVGQLAVSDKLPSIAMRDPNDAYGEVVSELVTGVTRDFRPTRVKQ"
     gene            complement(791..925)
                     /locus_tag="VFB89_07820"
     CDS             complement(791..925)
                     /locus_tag="VFB89_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27249.1"
                     /translation="MRGLVRLLELAAFLILVWLAISALRLMSGEPWPSIMHRPFGFFP
                     "
     gene            complement(928..1275)
                     /locus_tag="VFB89_07825"
     CDS             complement(928..1275)
                     /locus_tag="VFB89_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27250.1"
                     /translation="MAHSLRPHGALASFALLLALAGCSSSGGSDWVTPAPAAGKTGTG
                     IYVTGVVHHVDVEGGFYVIRGEDGVTYDPTNLPQEFQKDGLSVEADVRKRDDAVGTHM
                     TGTIVDLVRIRKR"
     gene            1308..2009
                     /locus_tag="VFB89_07830"
     CDS             1308..2009
                     /locus_tag="VFB89_07830"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HZM27251.1"
                     /translation="MKTLGIIGGIAPESTVEYYRQTIARYRERTAGGDYPSIIINSID
                     LKRLLALVAGGQLEELTDYLLAELSRLVSAGAQVGLLASNTPHIVFGELRARCPLPLI
                     SIVEAAAGATRARGFRRVGLLGTRSTMESRFYPDAFGTQGIEVIAPFPDERDYVHGRY
                     VGELVNGVFRDDTRQGVLAILDRMRQRDRVEAVILGGTELPLLFREGPAPNLPLLDTT
                     RIHVDRAVEAMLDSA"
     gene            complement(2022..2780)
                     /locus_tag="VFB89_07835"
     CDS             complement(2022..2780)
                     /locus_tag="VFB89_07835"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HZM27252.1"
                     /translation="MPRPQLITLMLVLACSHSSQPAPENRSLSGTVAYRERMALPSGA
                     VIQVQLSDVSVQDVAAPVVAETTVTPQGRQVPLPFELNYNAGKIDPKRIYAVRATIRS
                     EGQLLFTTTTATHVLTQGNPSRVDLVLIRAAAQSAAAGGMLSGTKWKLEDLGGTAVLD
                     QPQATLEFPEEGRVAGQGSCNRFFGSVQLSGDSISFGALGSTRMACLDAVMSQESAYF
                     RALQDAERYAIDGPSLLIYSRGTDKPLRFSRTGS"
     gene            complement(2795..>3260)
                     /locus_tag="VFB89_07840"
     CDS             complement(2795..>3260)
                     /locus_tag="VFB89_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27253.1"
                     /translation="YFQGLYQPGRSLMAIAAHAFSSRLSLGVVLLLACSRPDASILER
                     AEASPDPASIFINRVWQVRASSAVEVGTLYTFLSGGTLVIASPQGTPSLGSWSYARDT
                     LTLVEEGLSHPADVLTLAPGEFRIRVRSPGPPVDITLAPAEIPPPEEMDATR"
BASE COUNT          612 a         1022 c         1055 g          571 t
ORIGIN      
        1 agctcgatga ccttgtagac gctctccggc atgcgatcgc tccagaaaaa ggtgatccag
       61 gtcgaacctc gtcacgggac aacgggatgg gtggcgcgcc tcttgccctc tgcgccggca
      121 tctccccacc aggtgcggca aagtggtctg gaatcgtgcg atacgacggg ctcgtgcgca
      181 atgggcactg gcggtttgcg gaaccctcgc gctttcggca tgcggctcga aatacgccct
      241 ggtcccgcca cggctcgatc tcgaaccgta cggtcgagtg gctctcgtta cgttttccgg
      301 cgaccagacc aataacgaac tgaacacgct cgcgacccag cgctttgcgg aggcggtgct
      361 cgccagtcag accggcttcg agctgctcga actcggtgcc gccgattcgt cgctcaagcg
      421 gattgctgcg accggcgacg gcgaggcgtt ggcacacgcg gtcggcaagg tgaagaacgt
      481 tcctgccgtc ttcgtgggtc atctcagcgt ttccggtgtc aagccgcatg gccggctctc
      541 gggggtcagc agtctcaagg tgcgcgcgtc ggtttcggcg cagttgagtg tgcagctgct
      601 ctcgacctcc accggcggaa ccgtctggcg ctcgagcgca acagcggatg gcaccgtcgg
      661 acagctggcc gtgtccgata agctgccctc gatcgccatg cgtgatccca acgacgccta
      721 cggcgaggta gtaagcgagc tggtgacggg tgtgacccgg gacttccggc ccacccgggt
      781 gaagcagtag ctaggggaaa aaaccgaacg gccgatgcat gatggacggc catggctcgc
      841 cgctcatcag gcgcagcgcg ctgatggcca gccacaccag gatgagaaac gccgccagct
      901 ccagcagccg gaccaggccc cgcacagtca gcgctttcgg atccgcacca ggtccacaat
      961 cgttccggtc atgtgggttc ctactgcatc atctcgcttg cggacatccg cctccacgct
     1021 gagcccgtcc ttctgaaatt cctgcggtag gttggtcgga tcgtaggtca cgccatcctc
     1081 gccgcgaatg acgtaaaatc caccctcgac atcgacgtgg tgaacgaccc cggtgacata
     1141 gatcccggtt cctgtcttgc cggcggccgg ggcaggggta acccagtccg atccaccgct
     1201 actgctgcag ccggcaagcg cgagcagcaa tgcgaacgaa gcgagtgcac cgtggggccg
     1261 cagggaatga gccatcgaag cgatctcctt cagcaaccga ggctgcaatg aaaacgttgg
     1321 gcatcatcgg gggaatcgcc ccggagtcca ccgtcgagta ctatcgccag acgattgcgc
     1381 ggtaccgcga gcgcacagct gggggcgatt atccctccat catcatcaac agcatagacc
     1441 tcaaacgact gctcgcgctg gtggccggcg gacaactgga ggagctgacc gattacctgc
     1501 tggctgagct gagccggctc gtctcagccg gtgctcaggt gggactgctc gcctccaaca
     1561 ctccccacat cgtgttcggc gagcttcggg ctcgctgtcc gcttccgctg atcagcatcg
     1621 tggaagccgc ggccggggca acccgtgcca ggggtttccg gcgagtcgga ctcctcggaa
     1681 cgcgctcaac tatggaaagc cgcttctacc ccgacgcgtt cggcacgcag gggatcgagg
     1741 tgattgcgcc gttcccggac gagcgggact acgtgcacgg tcgctacgtc ggcgagctgg
     1801 tcaacggtgt cttccgggat gacactcggc aaggtgtttt ggcgattctc gaccggatgc
     1861 ggcagcggga tcgcgtcgaa gcggtgattc tgggcggaac cgagctaccg ctgctctttc
     1921 gagaaggccc agcgcccaac ctgccgttgc tcgacaccac caggattcac gtcgaccggg
     1981 cggttgaagc gatgctcgac agcgcctgac aacgcgagtg gtcaggagcc ggtgcgactg
     2041 aagcggagcg gcttatcggt gcccctggag tagatcagga gggagggtcc atcgatcgca
     2101 taccgctctg catcctggag ggccctgaag taggcggact cctgcgacat cacggcgtca
     2161 aggcaggcca tgcgggtgga gcccagcgcg ccgaagctga tcgagtcacc ggaaagctgg
     2221 accgatccga agaagcgatt gcaggagccc tggccggcaa ctcttccttc ctcgggaaac
     2281 tccagggtag cctgaggctg atccaggacc gccgtcccgc ccaggtcttc cagcttccac
     2341 tttgtgcccg aaagcatgcc tcctgctgca gccgactgag cagctgccct gatgagcacg
     2401 agatctaccc ggcttggatt gccctgggtg agcacatgcg tggcggtcgt cgtggtgaac
     2461 aggagctgcc cctcgctccg aatcgtggct cgaacagcgt agatcctttt agggtcgatc
     2521 ttgccggcgt tgtagttcag ctcgaagggc agcggtacct ggcggccttg cggagtgacc
     2581 gtggtttccg caacaacggg cgcggcgaca tcctgcaccg aaacatccga cagctggacc
     2641 tggatcacgg caccggaagg cagtgccatc cgctcccgat atgctaccgt gccgctcaat
     2701 gaccggtttt caggtgcggg ctgggaactg tgagaacagg cgagcaccag catcagcgtg
     2761 atcagctgcg ggcgaggcat gatggggcag cgcgtcaccg agtcgcgtcc atttcctccg
     2821 gcggcgggat ttccgccggt gcgagcgtga tgtcgactgg cggtccgggg ctccggactc
     2881 tgatccggaa ctcaccgggc gctaacgtca gtacgtcggc cggatgcgaa aggccttcct
     2941 cgaccagcgt cagcgtatcc ctcgcgtagc tccacgagcc taacgatggc gttccctgtg
     3001 gagacgcgat gacgagcgtc ccaccggaga gaaacgtgta caaggttccg acctcgaccg
     3061 ccgaagaggc acgtacctgc cacacccggt tgatgaaaat gctggccgga tcaggcgaag
     3121 cttcggcgcg ctcgaggatc gacgcgtccg ggcgagagca agcgagtagg agtacgacgc
     3181 cgagcgagag gcggctcgaa aacgcatggg cggcaatggc cataagcgat ctgccaggtt
     3241 gatacaggcc ctggaagtaa
//
LOCUS       DASRJQ010000355         9467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1461011, whole genome shotgun sequence.
ACCESSION   DASRJQ010000355 DASRJQ010000000
VERSION     DASRJQ010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 9467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9467
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461011"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /locus_tag="VFB89_07845"
     CDS             complement(<1..1149)
                     /locus_tag="VFB89_07845"
                     /inference="COORDINATES: protein motif:HMM:NF019375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HZM27254.1"
                     /translation="MLQATAGRLPEVVQAPTGSYYVFDAMSQIRYLIIADGDFGPMSS
                     KTANAIIRFQPERVVAVMDRRFAGQTVESALGFGGAIPIVDSVRHGLALGANTVLIGI
                     APVGGRLPDEWRSWLAEALDAGCDLVSGLHTFLSDDPVLAGKAKAAGRQIFDLRRPPT
                     DLAVASGLVKKVEAYVVLTVGTDCNVGKMTAQLELTRELNARGVRTRFVPTGQTGIML
                     EGWGIAVDAVVADFIAGAAERITLEGARDADVVLVEGQGSINHPGYSGVTLGLLHGAC
                     PDGLILCHQASREYIGDYGEADWLRIPSLADYVKVYETIGSMVHPTRVLGVCLNTYDL
                     PEAEARRACEAAVRETGLPVTDPVRFDSAPLVNAVLEGKARKQARQGTP"
     gene            1176..3869
                     /locus_tag="VFB89_07850"
     CDS             1176..3869
                     /locus_tag="VFB89_07850"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM27255.1"
                     /translation="MPDLRALLESGLAGHYTLGRELGRGGMATVFLAHDLKHDRPVAL
                     KVLHPELAASLGPDRFQREIRLAARLQHPHILSVHDSGETAGQLWFTMPFVEGESLRH
                     RLARERQLPLEDALRITREAALALEYAHQHGVIHRDIKPENILLTKDGSTLVADFGIA
                     RGFSADSDQLTETGLSVGTPAYMSPEQAAGERALDGRTDIYSLGIVLYEMLAGEPPYT
                     GATLQVIVARRLSEAPRPLRAVRETIPDSIDQAVQKALAKAPADRFTTAAQLAQALTL
                     TVTTPAATPTILPTAPPTIAPSVATQPAAEPPSARELRRRRRVPIGLATLGVGFLLGL
                     GVLFAWRRSHAGGDVKGPKHLAVLPFENQGDSADAYFADGITDDLRGKLAAVPGIEVV
                     AGRSSNKYRQTTKDLPDIAKDLGVEYLLVGKIHWTKGQAASRVRVSPELIWVRAGAAP
                     TTKWQQPFDAALTDVFQVQADIAGRVAQALNVALGSGASQALAARPTKSPEAYDLYLR
                     ANEYFNRNNKDDNEIAVELNDRAIRLDSGFALAWAKLAQSHAGAYWQHWDPSPQRLTL
                     AKQAAERALALQPDLPEAHLAMGFYHYWGHRDYDRALAEFAVTQRMQPNNVDAIEAVG
                     LVERRQGKWQEALASMRRAVELDPLNYSNLVELAGTYMLIRDYGEGAQLADRAIALAP
                     DVPGAYGAKALIYLNWVGNLEQVRNILREALRYNELGRVVDQAPTPAVMVLATDSTQR
                     SAILTLGARGFGKDLLTYFEIKARVYDLNGDIGPARTYYDSLALTAKAELKEQPDEAL
                     THAALGVAQAHLGHRDSAIREGRRAVELLPLTKDAYFGTIAQALLAEVYMVVGQPDEA
                     IDQLHALLLTPSVVSRAGLKASPIWAPLRGNPHFEQLVSGQ"
     gene            complement(3941..4711)
                     /locus_tag="VFB89_07855"
     CDS             complement(3941..4711)
                     /locus_tag="VFB89_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HZM27256.1"
                     /translation="MGIFDRLSTMIRSNINDLITRAENPEKMLNQLIVDMKTQLAKAK
                     QQVASAIADEKKLQSDAEAMKKQAEDWERRAMLAVQENRDDLAKQALMRYNEQLQGAQ
                     QLHETWLKHKAETEQLKLALRQLNDKIEEAKRKKNILIARAKRAEATQRIQETMSGMS
                     DRSAFESFDRMAEKIEATERKALAAAELQEEVSGDTLAKQFEALEYHGTADQQLLALK
                     QRMGLLPGGANRAKQLGTGANGVHDAEVIEESSGDTKP"
     gene            complement(4718..5113)
                     /locus_tag="VFB89_07860"
     CDS             complement(4718..5113)
                     /locus_tag="VFB89_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27257.1"
                     /translation="MITREDIQGWLDRLEGGTVSVEEPEPNLWLVRTTEGAEVVVHYA
                     PPVVILRVRVMELPASEPRRSELFRQLLELNARELVHGSYGLEGDDIVLIDTLELENL
                     DFSEFEASFDSITLALATHLGALAPYREG"
     gene            complement(5110..5361)
                     /locus_tag="VFB89_07865"
     CDS             complement(5110..5361)
                     /locus_tag="VFB89_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27258.1"
                     /translation="MNNDVLVILAIVLAVVVVKLVRGPLGQALADRVRGSAPPDPGVL
                     EELEAVKGRLAEVEERLDFAERLLAKSEQAERLPGRSDA"
     gene            complement(5377..5634)
                     /locus_tag="VFB89_07870"
     CDS             complement(5377..5634)
                     /locus_tag="VFB89_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27259.1"
                     /translation="MGLDGIMELIVWTTTAAVISAVTVVFALPTGRSWIRQVSKRAGH
                     EAANTEARLTAMQDRISELEERVDFAERMLTKQREPDRLES"
     gene            complement(5648..5986)
                     /locus_tag="VFB89_07875"
     CDS             complement(5648..5986)
                     /locus_tag="VFB89_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27260.1"
                     /translation="MSRKDADNPVLLTICLIGCFLAVGFTIVAVNVGEDFAIPVGITA
                     FVAAAVILRGPLGKALARRIEGRQEPGDNEALLQAMEELRGQMAELEERVDFAERVMA
                     QHREPDRLQQ"
     gene            complement(5983..6291)
                     /locus_tag="VFB89_07880"
     CDS             complement(5983..6291)
                     /locus_tag="VFB89_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27261.1"
                     /translation="MAIPPGDVAPIVITIVITVAVAGTAILRGPVGKGLARAMELGAG
                     GSTPPENDARIQRLEQRVSELEAAQGRLAELEERVDFAERLLTRNEAATRLPGPRGSE
                     "
     gene            complement(6307..6555)
                     /locus_tag="VFB89_07885"
     CDS             complement(6307..6555)
                     /locus_tag="VFB89_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27262.1"
                     /translation="MEDILALIFIFGGGTAIVLAFSPIGRALADRIRHREPIAAPDPA
                     MQEELQRLRDEVSELQERVDFAERLLAQRNEPTRLPAS"
     gene            complement(6556..6855)
                     /locus_tag="VFB89_07890"
     CDS             complement(6556..6855)
                     /locus_tag="VFB89_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27263.1"
                     /translation="MGELVPIAGMFTGLLITIAGVWGIVQIIQGPLGQALARRIQGRT
                     GDADLQNEVADLKDQIDGVRRQLEETQERLDFAERLLSQRPTTARIPGGDESGSA"
     gene            7208..7744
                     /locus_tag="VFB89_07895"
     CDS             7208..7744
                     /locus_tag="VFB89_07895"
                     /inference="COORDINATES: protein motif:HMM:NF018257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfocyanin-like copper-binding protein"
                     /protein_id="HZM27264.1"
                     /translation="MRRFSVLTLLLILGITVIGAAQTTDTSSAKGGDGSKALSYDAAT
                     KTVTFGLVAGAPGGKGGPFNFNGYTSGAATLTVPAGSKVVMNFVNEDGTPHSAVVVGG
                     DGALPNIGGDPAIPGAYTRDVTQGLAQFGKDVLKFTAPANGTFRIICGVPGHALSGMW
                     IWLKIDPATKTPSFGESK"
     gene            complement(7809..>9467)
                     /gene="uvrA"
                     /locus_tag="VFB89_07900"
     CDS             complement(7809..>9467)
                     /gene="uvrA"
                     /locus_tag="VFB89_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZM27265.1"
                     /translation="ALEEYMVEQPCQACGGKRLRPESLSVLVNGASIGDVVDLPVSRA
                     VTFFENIPQRGNGRAGVDPEIAGPILKEVSDRLRFLRDVGLDYLTLGRAAMSLSGGET
                     QRIRLATQIGSRLVGVLYILDEPSIGLHQRDNERLLGTLKGLRDLGNTVIVVEHDEDT
                     IRSADHVIDLGPRAGRLGGEVVAEGAVEDILRHPTSLTARYLRGDLRIPVPAGRRLAD
                     RKRKLRIVGARANNLRNLTVDIPLGLFVAVTGVSGSGKSSLVTDILYRALARHFYRAK
                     VVPGAHTRLEGLDQIDKVIDIDQSPIGRTPRSNPATYTGLFTPIRELFTALPEAKIRG
                     YGPGRFSFNVKGGRCEACQGDGLVKIEMHFLPDVYVPCEVCKGRRYNRETLEVRYKGR
                     SIADVLDLTVADALEFFANQRRIAEKLDLLNDVGLGYIHLGQAATTLSGGEAQRVKLA
                     TELSKRDTGRTLYILDEPTTGLHFEDVRLLLEVLHRLVDKGNSVVVIEHNLDVIKTAD
                     WIIDLGPEGGDGGGRVVAQGTPEEVGANQDSYTGRFLKKKLREQ"
BASE COUNT         1685 a         3156 c         2866 g         1760 t
ORIGIN      
        1 cggtgtgcct tggcgtgctt gtttacgcgc tttcccctcc aacacggcgt tcaccagtgg
       61 ggcagaatca aaacgaaccg gatccgtgac ggggagcccg gtctcgcgga cagcggcctc
      121 gcaggcacgc cgagcctcgg cttcaggcag gtcataggtg ttgagacaga ccccgagcac
      181 ccgagtggga tgcaccatcg agccaatcgt ctcgtagacc ttcacatagt cagcgagtga
      241 tggaatgcga agccagtcag cctcaccata atccccgatg tattcccggc tcgcctgatg
      301 gcagagaatc aatccatccg gacaggcgcc gtgaagcagt ccgagcgtca cgccggaata
      361 acccgggtga ttgatgctcc cctgcccttc taccagcacc acgtccgcgt cacgcgcgcc
      421 ctcgagcgta atcctttcag cagcgccggc gatgaagtcc gccaccacgg catccaccgc
      481 gataccccat ccctccagca tgatgccggt ctgcccggta gggacgaatc gggtgcgcac
      541 gccgcgcgcg tttagctcgc gggtcagctc gagctgagct gtcatcttcc cgacgttgca
      601 atcggtccca acagtgagaa cgacgtaggc ttcgactttt ttcaccagcc ctgacgcaac
      661 agccagatcc gtagggggcc gcctcagatc gaagatctgc cggcccgcgg ccttggcctt
      721 gcccgcgagc accggatcgt cgctcagaaa tgtgtgtaac ccgctgacca gatcgcaccc
      781 tgcgtcgagg gcttcggcca gccagcttcg ccattcatcc ggcagccgcc ctcccaccgg
      841 cgcaatcccg atcagcacgg tgttggcgcc cagggccagg ccgtgacgca ccgagtcaac
      901 gatgggaatc gcgccgccga agccgagcgc tgattcgacg gtctgcccgg caaagcgccg
      961 gtccatgacg gccacgaccc tctccggctg aaaccggatg atggcgttgg ctgtcttcga
     1021 gctcatcggg ccgaagtcgc cgtcggcgat gatcagatag cggatctggc tcatggcgtc
     1081 gaaaacatag taggagccgg taggggcctg aactacctcg gggagcctcc cggcggtagc
     1141 ttggagcatc ctcaatcacc cgtggctggg atcgggtgcc agaccttcga gctctcctcg
     1201 aaagcggctt ggcaggccac tacacgctcg ggcgtgagct tgggcgcggc gggatggcca
     1261 cggtgttcct cgcgcatgat ctcaagcacg accggccggt cgctctcaaa gtcctgcatc
     1321 ctgaactcgc agccagtctc ggacccgacc ggttccagag agagatccgc ctcgccgcac
     1381 ggctccagca ccctcatatc ctgtcggtcc acgattccgg agaaactgcg ggccagctct
     1441 ggttcaccat gcccttcgtg gaaggcgaaa gcctccgaca tcgactggct cgcgaacggc
     1501 agctgccgct cgaagacgcg ctccgcatta cccgggaagc ggccctcgct ctcgagtacg
     1561 cccaccagca cggagtgatc catcgggaca tcaagccgga gaacattctg ctcacgaagg
     1621 acggcagtac tctcgttgca gacttcggca ttgcccgcgg cttttccgcc gacagtgacc
     1681 agctcaccga gacggggctg tcggtcggga cccccgccta catgagtcct gagcaggctg
     1741 cgggcgagcg cgccctcgat ggccgcaccg acatctatag cctgggcatc gtcctctacg
     1801 aaatgctggc gggcgagccg ccttacaccg gggcgaccct gcaggtcatc gtggccagac
     1861 gcttgagcga ggcaccccgg ccgttacgag cggtgcgcga gaccattccc gattcgatcg
     1921 accaggcggt gcagaaggca ctggccaagg ctccagccga ccgcttcacc accgctgccc
     1981 agcttgccca ggccctcacg ctgacggtga cgacgcccgc agcgacgccg acgattttgc
     2041 cgaccgcgcc gccgacgatt gcgccgtcgg tcgccacgca gccagccgcc gagccaccta
     2101 gcgctcgcga gctacggagg agacgccggg ttccgattgg tctggcgacg ctcggggtgg
     2161 gcttcctgct cggtttgggc gtgcttttcg cgtggcggcg cagccatgct ggcggcgacg
     2221 tgaaggggcc caagcatctg gccgtgctcc cgttcgagaa ccagggcgac tcagcagatg
     2281 cctactttgc cgacgggatc actgacgacc tgcggggaaa gctcgccgcc gtgcccggga
     2341 tcgaggtcgt cgctgggcgc agctccaaca agtatcgcca gacgacaaag gacttgccgg
     2401 atattgcaaa ggacctgggc gtcgagtacc tgctggtggg taaaatccac tggaccaaag
     2461 gtcaggcggc aagccgggtg cgtgtgagcc cagagctcat atgggtgagg gccggagccg
     2521 cgcccaccac caagtggcaa cagccgttcg atgccgcgct gaccgacgtc tttcaggttc
     2581 aggcagacat cgccggtcgc gtggctcagg cgctcaatgt cgcacttggg tccggcgcgt
     2641 cgcaggctct tgcggcgcgc cccaccaaga gccccgaggc atacgatctc tacctgcgcg
     2701 ccaacgagta tttcaatcgc aacaacaaag acgacaacga gatcgcagtc gagttgaacg
     2761 atcgagccat ccggttggac tccggatttg ccctggcgtg ggcgaagctg gctcagtcgc
     2821 acgctggcgc ttattggcag cattgggacc cttcacctca acgtctgacc ctggcgaaac
     2881 aggcagcaga gcgcgccttg gcgctccaac cagatcttcc cgaggcccat ctggcaatgg
     2941 ggttttatca ctattggggt catcgcgact acgatcgggc gctcgcggaa ttcgccgtta
     3001 cgcagcggat gcagccaaac aatgtcgacg ccatcgaggc ggtgggcctg gtcgagcggc
     3061 gccaaggtaa atggcaggag gcgctcgcct caatgaggcg tgctgtcgag ctggacccac
     3121 tcaactattc gaacctcgtc gagttggcgg ggacttacat gctaatccga gactatggag
     3181 agggtgcaca gctagccgac cgcgctatcg ccctcgcacc ggatgttccg ggtgcttacg
     3241 gtgcgaaagc cctcatatac ctcaactggg tgggtaatct cgaacaggtg cgcaatatcc
     3301 tccgcgaggc cctccgctac aacgagcttg gaagggtcgt cgaccaggct cctacaccag
     3361 cggtgatggt cctcgcgacc gattcgaccc aaagatcagc tatcctgacg ctcggagctc
     3421 ggggctttgg aaaggatctg ctcacgtact tcgagatcaa agcaagagtg tacgacctca
     3481 atggcgacat tgggccggcc cgaacctact acgactcgct cgcccttacc gccaaggcgg
     3541 aactcaagga gcagccagac gaggcactga ctcacgcggc attgggagtc gcccaagctc
     3601 acctcggcca ccgggattct gcgattcggg agggaaggcg agccgtcgag ctgctaccgc
     3661 ttacgaagga cgcctacttc gggaccattg cccaggcgct actcgccgag gtctacatgg
     3721 tggtcggaca gccggatgaa gcgatcgacc agctgcacgc cctgttgctg acgccctcgg
     3781 ttgtctctag agcgggactg aaggccagtc cgatctgggc gccgcttcgc ggtaatcctc
     3841 atttcgagca gctcgtctcc ggacaataac ggcctccaaa actcaagcgg ggtcgtcctg
     3901 ctggacgacc ccgccgtagc tccgcccggt tcgccggacg tcagggcttg gtgtctccgc
     3961 tgctttcctc gattacctcg gcgtcgtgca cgccattggc acccgtgccg agctgtttcg
     4021 cccggttggc gccacccggc aagagtccca tacgctgctt gagcgcgagc agctgctggt
     4081 cggcggtacc atggtactcg agcgcctcga actgtttcgc gagggtgtcg ccggagacct
     4141 cttcctgcag ctcggccgcc gcgagggcct tccgctcggt cgcttcgatc ttctcggcca
     4201 tccgatcaaa ggactcgaac gcactccggt cggacatgcc ggacatggtc tcctgaatgc
     4261 gctgcgtggc ttcggcccgc ttggcgcgcg cgatcaggat gttcttcttc cgcttggcct
     4321 cttcgatctt atcgttgagc tggcgcagcg cgagcttgag ctgctcggtc tcggccttgt
     4381 gcttgagcca ggtctcgtgc agctgctggg caccctgcag ctgctcgttg tagcgcatga
     4441 gcgcctgctt ggcgagatcg tcccgatttt cctgcaccgc aagcatggcg cgccgttccc
     4501 agtcctcggc ttgcttcttc atcgcctcgg cgtcggactg gagtttcttc tcgtctgcga
     4561 tggcggacgc gacctgctgc ttggccttgg ccagctgcgt cttcatgtcg acgatcagct
     4621 ggttgagcat cttctccggg ttttcggccc gggtgatcag gtcgttgatg ttggaccgaa
     4681 tcatggtgga cagccggtcg aagattccca tcgcggatca gccttcccgg tagggcgcca
     4741 gggcgcccag atgcgtggcg agagccagcg taatcgagtc gaagctggcc tcgaactcgc
     4801 tgaagtccag gttttccagc tcgagcgtgt cgatgaggac aatgtcgtcg ccctcgagcc
     4861 catacgaccc gtgcaccagc tcacgtgcgt tcagttcgag cagctggcga aacagctcgc
     4921 tgcgccgggg ctcgctggcc ggcagctcca tcacgcgcac gcgaagaatg acaaccggag
     4981 gggcgtagtg cacgaccact tcggcaccct cggtggttcg aaccagccac agattcggct
     5041 cgggctcctc gacggacacc gtgccgcctt cgaggcgatc gagccaaccc tggatgtctt
     5101 cgcgggtgat catgcgtcgc tcctcccggg gagccgttcc gcttgctcgc tcttcgccag
     5161 caaacgctcg gcgaaatcta accgctcctc tacctcagcc aaccgccctt tcaccgcctc
     5221 caactcctcc agcactcccg gatccggcgg agccgagccc cgaactcgat cagcgagcgc
     5281 ctgtccgagc gggccacgga ccagcttcac cactaccacc gccaagacaa tggcgagaat
     5341 gaccaggaca tcgttgttca cggctgggct cgactcctag ctctcgagac gatccggttc
     5401 ccgttgcttc gtcaacattc tctccgcgaa gtccacccgt tcctccagct ccgaaatccg
     5461 gtcctgcatg gccgtcaagc gagcctccgt gttggccgct tcatgaccgg ctcgtttcga
     5521 tacctggcga atccaggagc gtcccgtcgg cagagcgaat accacggtca cagcgctgat
     5581 aaccgcggca gtcgtggtcc agacgataag ctccattatg ccgtcgagtc ccatgaacgc
     5641 cttccatcta ctgctgcagc cggtccggct cccgatgttg cgccatcacc cgctccgcga
     5701 aatcgacccg ttcctccaac tccgccatct ggccgcgcag ctcctccatt gcctggagca
     5761 acgcctcgtt gtcccctggc tcctgccgcc cctcgatccg ccgcgccaat gcctttccta
     5821 acgggccgcg cagtatcacc gccgctgcga cgaacgctgt aattccgacc ggaatcgcga
     5881 agtcctctcc cacgttcact gcgacaatcg tgaacccaac cgccagaaag catccgatca
     5941 agcagatcgt caggaggacc ggattatctg cgtccttccg actcattcgc ttcctcgtgg
     6001 acccggcagc cgagtcgctg cctcgtttcg ggtcaagagc cgctccgcga agtccacccg
     6061 ctcttcgagc tcggccagcc gcccctgggc tgcttcgagc tctgaaacgc gctgctcgag
     6121 tcgttggatc cgggcatcgt tctcaggcgg cgtcgaaccc ccagcgccga gctccatggc
     6181 gcgtgccagt cctttgccga ctggtccgcg gagaatcgcg gtgccggcca ccgcgacggt
     6241 gatcacgatg gtgatgacga tcggcgctac atcaccaggt ggaattgcca tgtacgcgaa
     6301 cctctctcag gaagccggga gccgggtcgg ttcattgcgc tgcgccagca gccgctccgc
     6361 aaaatccacc cgctcctgca gctccgacac ctcatcccgc agtcgttgca gctcctcttg
     6421 catcgccgga tccggcgccg ctatcggctc gcgatgtcgg attcgatccg ccagcgcgcg
     6481 accaatcggg ctgaaggcga ggacgatcgc cgtgcctccg ccgaagatga agatgagggc
     6541 gaggatgtct tccatttacg cactaccgct ttcatcgccg ccggggatcc gggcggtggt
     6601 cggccgctgg ctcaggagcc gctcggcgaa atcgagccgc tcctgtgtct cctcgagctg
     6661 ccgacgaacc ccgtcgatct ggtctttgag atcggctact tcgttctgga gatcggcatc
     6721 gcccgtgcgg ccctgaatcc gtcgagccag ggcctggccc agtggcccct ggatgatctg
     6781 cacgatgccc cagactccag ctatcgtgat caggagtccg gtgaacatcc cggcaattgg
     6841 aaccagctcg cccattggat ctttccgtga ttcgccaggg ctccaaatcg gcccaatcga
     6901 tacccgcgac aggtgttacg gagccccgtt gcgaaatgtt tcaattcgag acgactttgc
     6961 cgctgaccgc tggtctacct tatgactccc gacgccccac ggtgtccccc gggcggggcc
     7021 agataccgac gacgtagcgc gtgatgccaa ggatcgacgg tccggtcata tccgggctaa
     7081 tgtaggacgc cgtcgaaaga ggcgcgagga gccggggcga gaccgatcga gtcgcttgtc
     7141 aggcctcccg gaaaccggca tgtgggttgc acatgcaccg aaccctgcta gaaccggcag
     7201 agagaccatg agacgctttt ctgtactgac gttgctgctc atccttggga tcacagtgat
     7261 cggcgccgcg cagaccaccg atacgagttc ggccaagggt ggtgacggct ccaaagcgct
     7321 tagctacgac gcagcgacca agaccgtcac ctttggcctg gtcgccggcg ctcccggcgg
     7381 aaaaggcggc ccgttcaact tcaatgggta caccagcggg gctgccaccc tgactgtccc
     7441 ggccggaagc aaggtggtca tgaacttcgt gaacgaggac gggacgccgc acagtgcggt
     7501 cgtagttggt ggcgatggag cgctgccgaa tatcggcggc gaccccgcga ttccaggcgc
     7561 gtacacgcgc gatgtgacgc agggattggc ccagtttggc aaggatgtgc tgaagttcac
     7621 cgctccagcc aacggcactt tccgcatcat ctgcggcgtt cccgggcatg ccctttcggg
     7681 catgtggatc tggctcaaga tcgatccagc cacaaagacg ccgagcttcg gggagagtaa
     7741 gtagaaactg cgtaaacggt aaacggtgaa cgggggctcc gccgatgcga ggagcccccg
     7801 ttcaccgttc actgttcacg tagtttcttt ttcagaaacc ttcccgtata gctgtcctgg
     7861 ttggcgccta cctcttccgg cgtaccttgg gcgaccactc gcccaccgcc gtcgccgccc
     7921 tccggtccca gatcaataat ccagtcggcg gtcttgatga cgtcgaggtt gtgctcgatg
     7981 acaaccacgc tgtttccctt gtccaccagg cgatgcagca cctcgagcag gaggcgtacg
     8041 tcttcaaaat gcaaaccggt ggtgggctca tccaggatgt acagcgttcg tccggtatcg
     8101 cgcttggaca gctcggtcgc gagcttgact cgctgggctt cgccgcccga cagtgtcgtc
     8161 gctgcttgcc cgagatggat gtagccgagt cccacgtcgt tgagcagatc gagcttctcg
     8221 gcgatgcggc gctggttagc gaaaaactcg agcgcgtctg ccacggtcag atcgagcacg
     8281 tctgcgatgc tgcgcccctt gtagcgcacc tcgagcgtct cgcggttgta gcggcgcccc
     8341 ttgcacacct cgcaaggcac gtaaacgtcc ggcaggaagt gcatctcgat cttcaccaga
     8401 ccgtcgccct ggcacgcctc gcatcgtccg cctttgacgt tgaaggagaa tcgccccggc
     8461 ccgtagccgc ggatcttcgc ctcgggcaac gcggtgaaca gctctcgaat gggggtgaag
     8521 aggccggtgt acgtggccgg gttcgagcgc ggagtgcgcc cgatcgggct ctggtcgatg
     8581 tcgatcacct tgtcgatctg atccaggccc tccagtcgtg tatgcgcacc gggcactacc
     8641 ttggcgcggt agaaatgccg cgcgagcgct cgatataaga tgtcggtaac caggctcgac
     8701 ttccccgagc ccgacacacc ggtcacggcg acgaacagtc cgagcggaat gtcgacggtg
     8761 agatttcgca ggttgttcgc tctggcgccg acgatgcgca gcttgcgttt ccggtcggcg
     8821 agccggcgcc ccgctggtac cgggatgcga aggtcgcccc ggaggtatcg cgcggtgagc
     8881 gaagtcggat gccgaagaat gtcctctacg gctccttctg ccacgacctc gccacccagc
     8941 cgtcccgccc gcggacccag gtcgatgacg tggtccgcgg accgaatagt gtcctcgtca
     9001 tgctcgacga cgatgactgt gttgccgaga tcacgcagcc ccttgagggt gcccagcagc
     9061 cgctcgttgt cgcgctgatg cagcccgatg ctgggctcgt cgaggatata gagcacgccg
     9121 accagtcgtg agccgatctg ggtggcgagg cgaatccgct gggtttcccc gcccgagagc
     9181 gacatggccg cgcgcccgag cgtgaggtag tccagtccca cgtcgcggag aaatcggagc
     9241 cggtcgctca cttccttcag gatgggcccg gcgatttccg gatccacgcc ggcgcggcca
     9301 tttcctcgct ggggaatgtt ctcgaagaac gtgacggcac gagagacggg cagatctacc
     9361 acatccccga tgctggcacc attcaccagc accgacagac tctccggccg aagccgcttg
     9421 ccgccacagg cctggcaggg ctgttccacc atgtactctt cgagcgc
//
LOCUS       DASRJQ010000356         3367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2154463, whole genome shotgun sequence.
ACCESSION   DASRJQ010000356 DASRJQ010000000
VERSION     DASRJQ010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2154463"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..870
                     /gene="recO"
                     /locus_tag="VFB89_07905"
     CDS             109..870
                     /gene="recO"
                     /locus_tag="VFB89_07905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HZM27266.1"
                     /translation="MPLVSTPAVILATLRYSDTSKIVRLATRDLGVQSAIAKGALRPR
                     SPFGAALQVLSDGQAQLYVKDNRELHTLAAFDVIHLRVGLSADLNRYAVAAAMAEVML
                     RFAPPDPHPESFGVLVDALESIEATESAYLPAAALRAMWHLVGALGFTPSLAGCVLCG
                     SIVVSDGDVAFSTADGGVLCTSCADLRAATRLPQRARWDLAHLIDPTCPLPLLDGPHF
                     AAHQRLLARYILYHLGEGTELPALDFWTRRPWMTR"
     gene            867..2711
                     /gene="selB"
                     /locus_tag="VFB89_07910"
     CDS             867..2711
                     /gene="selB"
                     /locus_tag="VFB89_07910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HZM27267.1"
                     /translation="MIVGTAGHIDHGKSALVTAITGHAVDRLAEERRRGITIDLNFAP
                     LDLGDGVVGGVVDVPGHEDFVRTMVAGASGFDLALLVVAADEGIMPQTEEHLAILEQL
                     AIPVGIPVITKADLVDPDWLELVVSELSERLRSSSVAFGPPAVVSAVTGQGIAELRAR
                     LRAHAVNQAPRARADLFRLPVDRAFSVAGVGTVVTGTAWSGSVAMGDAVRLLPGGVDA
                     RVRSIESHGHAVSQTEPGARIALGLAGIERSQVARGQVLVTPDAPWSATTAVDVELAL
                     LSSAERPLVARTRARLHLGTTEVMVRVLPRSPIRPGERGLARLRLEAPAVARGGDRFV
                     LRSWSPVTTIGGGRVLDPDPPLRRATWPATLAAAEPRERLRALVERRGNGLSLDAVPI
                     VVGSGSQLEALPEGINGLRLVGRVLIATDVVDRTSDRAVEMVDDFHRGHRSDPGMPLE
                     TLRRGLRVPDWLADTAIQSATAAGRLVVEAGTVRRPGFVARVAGGEQEVQRLVQIVQV
                     AGLTPPTVAELERETGRRDVAAALRLAAARGLVEAVERDRYYATEALQKFSETVREIG
                     RAGDIQPAAIRERLGISRKYLIPLLEWADARGITVRAGDIRRLARERN"
     gene            2866..3303
                     /locus_tag="VFB89_07915"
     CDS             2866..3303
                     /locus_tag="VFB89_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HZM27268.1"
                     /translation="MVRFRWAGWWLGLMVGAGSPVAAQVLAPAPKVELEDFYPNPLGA
                     ATTIPFKINPEVCVRGHQPVVSLRIFNSLVQVVAIPVLASGNGQPIDFVRVPCGEHQA
                     YWNGKYSDGKREAGAGVYYYQLTVDGERYTRKMVVLRRIPPPH"
BASE COUNT          537 a         1048 c         1129 g          653 t
ORIGIN      
        1 ccgcgctcgt gttgtgggaa ggatgatagg aagtgggacc aacggaggca aggtgtcccg
       61 cgaaccgagc gccgatggct gatagctgat agctgatagc tttaacccat gcccctcgtc
      121 tccactccgg ccgtcattct cgccacgctc cgctatagcg acacgtccaa aatcgtgcgg
      181 ctggcgaccc gcgacctcgg cgtccagagc gcaatcgcca agggggccct tcggccgcga
      241 agcccgttcg gggccgcgtt gcaggttttg agcgatgggc aggcccagct ctatgtgaag
      301 gacaaccgag agctgcacac gctcgcggcc tttgacgtca ttcacctgag agtcgggttg
      361 tccgccgatc tcaatcggta cgcggttgcg gccgcaatgg ccgaggtcat gctgcgcttt
      421 gcgccaccgg atcctcatcc cgagagcttt ggggtgcttg tcgacgctct cgaaagcatc
      481 gaggcaaccg agtcggcgta ccttccggcg gccgcactgc gagccatgtg gcacctggtc
      541 ggtgcgctcg gattcactcc gtcgctcgct ggttgcgtct tgtgcggcag catcgtcgtg
      601 agcgatggcg acgtcgcctt cagcaccgcg gatggtggcg ttctttgtac ctcctgcgcc
      661 gacctccgtg cagcaactcg gcttccccaa cgggccagat gggaccttgc ccatctcatc
      721 gatccgacct gcccgcttcc gctgttggat ggacctcatt tcgcggccca tcaacggctc
      781 ctggcccggt acattctcta tcaccttggt gaggggactg agctgccggc gctcgatttc
      841 tggactcggc ggccctggat gactcggtga tcgtcggtac ggccgggcac atcgatcacg
      901 gcaagtccgc actggtaacc gcaattaccg ggcacgcggt ggaccggttg gccgaagagc
      961 gccgacgcgg catcaccatc gatctcaact ttgccccgct cgaccttggc gatggcgtcg
     1021 tcggcggcgt ggtggacgtc cccggccacg aggatttcgt ccggaccatg gtggccggcg
     1081 cgtccgggtt cgacctggcc cttctcgtcg tggctgctga cgaaggcatc atgccccaga
     1141 ccgaagagca tcttgcgatt ctggagcagc tcgcgattcc ggtgggtatc ccggtcatca
     1201 ccaaggcaga cctggtagat cccgattggc tggaattggt ggtttcggag ttgagcgagc
     1261 gattgcgcag ctcatcggtc gcgttcggcc cgcccgcagt cgtgtcggcc gtaaccgggc
     1321 agggaattgc ggagcttcga gctcggcttc gagctcacgc cgtcaatcag gctccgcgtg
     1381 ctcgagccga tctcttccgg cttcctgtcg atcgggcatt ttccgtcgcc ggggtcggta
     1441 cggtggtgac gggcaccgca tggtcgggat cggtcgcaat gggcgatgca gtgcgcctcc
     1501 tgccaggggg tgtcgatgcc cgcgtgcgtt cgatcgagtc tcacggtcac gctgtgagcc
     1561 agaccgagcc tggcgctcgc atcgcattgg gactcgccgg aatcgagcgc tcgcaggttg
     1621 cgcgagggca ggttctggtc acccccgatg cgccctggtc ggcgacgaca gcggtcgacg
     1681 tcgagcttgc acttctctcc tcggcggagc gcccattggt agctcgcacg cgcgcccgtc
     1741 ttcacctcgg aacaacggag gtaatggttc gagtgctgcc acgcagcccc atccgtccgg
     1801 gtgagcgcgg cctggcgcgg cttcgcctcg aggcacccgc ggtggcgcgc gggggcgatc
     1861 gatttgtact gcggagctgg agcccggtca ccaccatcgg tggtggtcgc gtgctcgacc
     1921 cggacccgcc gctccggcga gcaacttggc cggctactct tgcggcggcc gagccgaggg
     1981 agcggcttcg ggcgctggtc gagcggcgcg gtaacggact ttcgcttgac gctgtcccga
     2041 tcgtcgtcgg ctccggatct cagctggagg ccctcccgga ggggatcaac ggcctgcgtc
     2101 tggtcggcag ggtgctgatt gcgaccgatg tcgtcgaccg gacatcggat cgagctgtgg
     2161 agatggtcga tgattttcac cggggccatc ggtcagatcc cggaatgcca ctcgagacgc
     2221 ttcggcgcgg gttgcgggtt ccggactggc tggccgacac ggccatccag tccgcaaccg
     2281 ccgccggacg attggtggtg gaggccggga ccgttcggcg tcccggattc gtggccagag
     2341 tcgccggcgg ggaacaggag gttcaacgcc ttgtacagat cgttcaggtt gccggcctca
     2401 cgccgcctac ggttgccgag ctcgaacgag aaaccggacg acgggatgtc gccgcggcgc
     2461 tccggctagc cgcggcgcgt gggctggtcg aagcggtcga gcgtgaccga tattacgcaa
     2521 ccgaagcgct ccaaaagttt tcggagactg tacgagagat cggacgtgcc ggcgacattc
     2581 aacccgccgc tatccgggag cgcctaggca tcagccgcaa gtatttgatt ccgttactcg
     2641 aatgggcaga cgctcgcggc atcaccgtgc gcgcgggcga tatccgacgg ctggcgcgcg
     2701 agcggaacta acgcagcagc tcactcgcgt tcatgtcgtt ttccttgaca gggcacttcg
     2761 cgcgtgggca tattccgcca ccctctccat tcgaagatcc aggttagatc cccatttgca
     2821 gggaggtgct gccaccggtc aacccttggt tcgacaggag tctccatggt ccgcttcagg
     2881 tgggcggggt ggtggctcgg tctgatggtg ggtgcaggct cgccagtggc cgcacaggtg
     2941 ttagctcctg cgccgaaggt cgagctggag gacttctacc ccaatccgtt gggcgcggcg
     3001 acaacgattc cgttcaaaat caaccccgag gtatgcgttc gaggccatca acccgtcgtc
     3061 agtcttcgaa tcttcaactc tctggtacaa gtcgtcgcga tcccggtgtt ggcctcggga
     3121 aatgggcagc cgatcgattt cgtacgggtc ccctgcggcg agcaccaggc gtactggaat
     3181 gggaagtaca gtgatggcaa gcgggaggct ggcgcgggag tgtactacta tcagttgacg
     3241 gtcgacggtg agcgctacac ccggaagatg gtggttttga ggcggatacc gccgccacac
     3301 tgaaagacag cgtaaacggt gaacagtgaa cggtgaacgg ggggtcccga cgtatgggtg
     3361 cctcccg
//
LOCUS       DASRJQ010000357         4801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1998365, whole genome shotgun sequence.
ACCESSION   DASRJQ010000357 DASRJQ010000000
VERSION     DASRJQ010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1998365"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="VFB89_07920"
     CDS             complement(<1..687)
                     /locus_tag="VFB89_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27269.1"
                     /translation="MTTIRRRLAFWYTVALGITVVAFGGALYLERGQSSVRELDRRLG
                     LESDLATRWLVESYRVLGSLIGGPTNPSLAPGISAYLEAFRDYLVLADTSHKVVALSG
                     VTRGLTAAELEQLSAPARTLTARRLAGTLDVGGSLGTMRYLATRVDSAGPGIGWLVVA
                     TPTEDVAFGPSALLQSMLVIAPIVLLCSAALGWWLAGTSVQPLLGIMDELDAISDGRS
                     LHRRLAVPLSG"
     gene            complement(689..1357)
                     /locus_tag="VFB89_07925"
     CDS             complement(689..1357)
                     /locus_tag="VFB89_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZM27270.1"
                     /translation="MKILVIEDDRTVGQYVKRGLDEHLYHADLVDDGLEGLRLASGGQ
                     YDLVVLDLRLPGMTGLEVLRTLRDRGITTPILVLTAQDAVDWKVQALRMGADDYVTKP
                     FAFEEVLARVEALGRRPKELRTPVLRVADLELDTATREVRRGGQLIELTPKEYTVLEY
                     LMRHPGRVMSRTLITEYAWDYHFDPGTNIVDVVINRLRKKVDSGHSGKLVHTVRGVGY
                     VVKG"
     gene            complement(1378..2904)
                     /locus_tag="VFB89_07930"
     CDS             complement(1378..2904)
                     /locus_tag="VFB89_07930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZM27271.1"
                     /translation="MPGTGGADVALLGAVAKLLNGSLGFDDAIRAVAETMRSGLPALR
                     VALWLREPTGTAFRPVSSPPADVPAVASLQQLNSTPSDGTIRVPLMQDGQCVGMIEAT
                     LDPGRQDPALLGVLADMLAPFVGSAELSADLAHEVATQSREIDEQRRFISLIIDSLPV
                     GLYVVDREYRIQAWNRKRETGTQGLRRDEVVGRPVFDVLSRHPRDRLRAAFDRVFDTG
                     EISQEEMDVGLGEEQRMYRITRIPMRLEGDAITHVITVGEDVTEQRLSERRILQSEKL
                     AAIGHLAAGVMHEINNPLATISACVEAMEGRLPDLSGTAVNSVRDYLQIVNKEVERCS
                     QIVNGLLDFSRPKGKAKQVVQLNAVVEDALFLLKHHQRFGQLVLTRELEPRPLLTVGN
                     AEQLIQVLMALVLNALDAMEKGGRLTVRTGRSRMRADEIFVEVEDTGIGIPRSQQLLI
                     FEPFFTTKPPGRGTGLGLSISYGIVQEHGGRIEVESQPGEGARFRVVLPASVEPKAER
                     "
     gene            complement(2934..3419)
                     /locus_tag="VFB89_07935"
     CDS             complement(2934..3419)
                     /locus_tag="VFB89_07935"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZM27272.1"
                     /translation="MIRLRPAAVACALVVAACRIEDHTPTGSRSDDEAIQHVLTQYTQ
                     GLSARDWPAVRSLFWEDATYQMTLPTRNLVLPIDSARPRLLPPGEPGDGRTFGVRILR
                     NDTRQEGDLAASWLVSRRTLTSGAGVMLESDWTEHVILRRVAGEWRILSIALAGGRHR
                     T"
     gene            complement(3481..4005)
                     /locus_tag="VFB89_07940"
     CDS             complement(3481..4005)
                     /locus_tag="VFB89_07940"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HZM27273.1"
                     /translation="MSFPPLAALLTPSPTFSTWSPMASLPPVGATAPDFTLPSTSGAE
                     VSLSGLRGKNVLLAFFPLAFTSVCTKEMGEFSTDYEQFRSANTEVLPISVDSIPTLKE
                     FKAKERLTVDLLSDFKREVSRKYGTLLEDKFFSNRAYVLIDKQGVVRWAFAESSTGNR
                     RENAELLTELEKLR"
     gene            complement(4031..4639)
                     /locus_tag="VFB89_07945"
     CDS             complement(4031..4639)
                     /locus_tag="VFB89_07945"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HZM27274.1"
                     /translation="MAFTLPPLPYPHNALEPHIDEQTMRIHHEKHHAAYVNNLNAALE
                     SHPDLQQKSLDDLLRNINSVPEAIRTAVRNNAGGHSNHTFFWEIMRPGGPKEPTGALA
                     DAITKNLGGFASFKEQFAKACVGRFGSGWGWLTLDKSGKLAIESTPNQDSPIMDGKTP
                     VLGCDVWEHAYYLKYQNRRPDYVAAWWNVVNWSEVGKRFDKK"
BASE COUNT          842 a         1572 c         1532 g          855 t
ORIGIN      
        1 gcctgaaagc ggcaccgcaa gccgccgatg caggctgcgg ccgtcgctga tggcgtcgag
       61 ctcatccatg atgccgagca agggctggac gctggtgccg gcgagccacc agccgagcgc
      121 ggcagagcac agcagcacga taggcgcgat gaccagcatc gactgaagca acgcgctcgg
      181 gccgaacgcg acgtcctcgg ttggcgtcgc caccaccagc cagccaatgc cgggacccgc
      241 cgaatctacc cgagtcgcga gatagcgcat cgtcccgagc gacccaccga catccagtgt
      301 tccggccaaa cgtcgagcag tgagcgtgcg ggccggcgcc gaaagttgct ccagctcggc
      361 agccgtaagc ccgcgcgtga cgccggagag cgccacgacc ttgtggctcg tatccgccag
      421 gaccaggtag tcgcgaaacg cttcgagata ggcgctgatg cccggggcca gcgagggatt
      481 ggtcgggccg ccgatcaggc ttcccagcac gcggtacgac tccaccagcc agcgggtggc
      541 gaggtcggac tcgagtccga gccggcggtc cagctcacgc acgctggatt gtccgcgctc
      601 gaggtacagc gcaccgccga acgcgacgac ggtgatgccc agcgccacgg tgtaccagaa
      661 ggcgagtcgc cggcggatcg tcgtcatctc agcccttcac cacgtagcct acaccgcgca
      721 ccgtgtggac cagcttgccg gagtgcccgg agtccacctt cttgcgcagc cggttgatga
      781 ctacgtcgac gatgttggtg ccgggatcga agtggtagtc ccaggcgtat tccgtgatca
      841 gggtacggga catgacccgc cctgggtgcc gcatgagata ctccagaacc gtgtactcct
      901 ttggagtcag ctcgatcaac tggccgcctc gccgcacctc gcgggtcgcg gtatcgagtt
      961 cgagatccgc gaccctcaga accggggtgc gcagctcctt gggtcgccga cccaaggcct
     1021 cgacgcgcgc cagcacctcc tcgaatgcga acggcttggt gacatagtcg tcggcgccca
     1081 tgcggagagc ctggaccttc cagtccacgg cgtcctgtgc cgtgagaacg aggataggag
     1141 tagtgatccc acggtcacga agggtgcgca ggacttcgag accggtcatg ccgggaagcc
     1201 ggagatcgag cacgaccagg tcgtactggc ctccggaggc gagtcgcaag ccttccaggc
     1261 cgtcgtccac cagatccgca tgatacaggt gctcatcgag cccccgcttc acgtactgac
     1321 ccaccgtacg gtcgtcctcg atcaccagaa tcttcaaccg acccggccct tccttcctta
     1381 ccgctccgct ttcggttcga ccgaggcagg caacaccacg cggaagcggg ccccttcgcc
     1441 cggctggctc tcgacctcga tgcggccgcc gtgctcctgc acgatgccgt agctgatcga
     1501 cagcccaagc ccggtgcctc gccctggcgg cttggtcgtg aagaaaggct cgaagatgag
     1561 cagctgctgg gagcggggga tgccgatgcc agtgtcttcc acttcgacga agatctcgtc
     1621 ggctcgcatt cggcttcggc cggtgcggac cgtgagacgc cccccttttt ccatggcgtc
     1681 gagcgcgttc agcacgagcg ccatcaacac ctggatcagc tgctcggcgt ttccaaccgt
     1741 gagcagcggc cggggttcca gctcgcgcgt cagcaccagc tgcccgaatc gctggtgatg
     1801 cttgagaaga aacagggcat cctccaccac cgcgttcaac tggactacct gcttcgcctt
     1861 gcccttcggt cggctgaaat cgagcaatcc attcacgatc tgggagcagc gctccacctc
     1921 cttattcacg atctggaggt agtcccgaac cgagttcacg gccgtgccgg acagatccgg
     1981 caggcgaccc tccatcgcct cgacgcacgc gctgatggtg gcgagcggat tgttgatctc
     2041 atgcatgacg ccggccgcca gatggccgat cgccgcgagc ttctcgctct ggaggatccg
     2101 gcgctcactc agccgctgct cggtcacgtc ttcgccgacc gtgatgacgt gcgtgatcgc
     2161 atcgccttcg agccgcatgg ggatacgggt gatccgatac atccgctgtt cctcacccag
     2221 gccgacatcc atctcttcct gggagatctc gccggtgtcg aagacccgat cgaacgccgc
     2281 tcgcaaccga tcccgaggat gccggctcag gacgtcgaat acggggcgcc ccaccacttc
     2341 gtcgcggcgc agtccctggg ttccggtttc ccgcttgcgg ttccaggcct ggattcgata
     2401 ctcccggtcc actacgtaga gtccgaccgg taggctgtcg atgatcaggc tgatgaagcg
     2461 ccgttgttcg tcgatttcgc gagattgggt tgcgacttca tgcgccaggt cggcggacaa
     2521 ctcggccgat ccgacgaacg gcgcgagcat gtccgccaac acgccgagca gcgctgggtc
     2581 ttgccgcccc ggatccagcg tcgcctcgat catcccaacg cattgcccgt cctgcatcag
     2641 ggggactcgg atcgtgccgt cggacggagt actgttgagc tgctggagcg acgccaccgc
     2701 cggcacatcg gccggcggcg agctgaccgg acgaaaagcc gtcccggtcg gctcccgaag
     2761 ccaaagcgcg acacgaagcg cgggcagacc tgaccgcatt gtttccgcga ctgcccggat
     2821 ggcgtcgtcg aagccaaggc tgccgttcag cagctttgca acagcgccca gcagggcgac
     2881 gtcggcgcct ccggttcccg gcacgcctgg cttatccgat ggcgacggtg acgtcacgta
     2941 cggtgccgtc cgcctgcgag cgcgatcgac aggatccgcc attcgccggc gacgcgtcgc
     3001 aggatgacat gttcggtcca gtcggactcg agcatcacac cggctccgga cgtcagcgtc
     3061 cggcggctca ccagccagga agcggcgaga tctccctcct gcctggtatc gttgcggaga
     3121 atgcgcacgc cgaaggtccg accgtcccca ggctcgcccg ggggcaggag ccgcgggcgg
     3181 gcggagtcga tcgggaggac caggttccgg gtaggaagag tcatctggta cgttgcgtct
     3241 tcccagaaca ggctgcgcac cgcaggccag tctcttgccg agaggccctg cgtatactgg
     3301 gtaagcacgt gctgaatcgc ttcatcgtcg ctccgggatc cggtgggagt gtggtcctcg
     3361 atgcggcacg cagccacgac cagcgcgcac gcaaccgcag cggggcgcag gcggatcatg
     3421 gggctccttg ggatctcgtt gatgtaaaga tatcgaaatg cgaggggaga aactgcgggt
     3481 tcaccgcagt ttctcgagct ccgtcaacag ctccgcgttc tcccgccggt ttccggtcga
     3541 gctctccgcg aacgcccatc gcacgacgcc ctgcttatcg atcaggacgt aggcgcggtt
     3601 ggagaagaat ttgtcctcca gcagcgtgcc gtatttccgg ctgacctccc gcttgaagtc
     3661 gctcaagagg tccaccgtca ggcgctcctt cgccttgaac tccttgagcg tcggaatgga
     3721 gtcgacgctg atgggcaaaa cctcagtgtt ggcggaacgg aactgttcgt agtcggtcga
     3781 gaactcgccc atctccttgg tgcacacgct ggtgaatgcc agagggaaaa atgccagcag
     3841 cacgttcttg ccgcggagac ccgagagcga gacctccgct cccgaggtgg agggcagagt
     3901 aaagtcgggg gcggttgcac cgacaggagg caaggaagcc atgggtgacc aggtcgagaa
     3961 ggttggtgaa ggggtcagga gcgctgcgag aggcgggaag ctcatccccc gcgcctctcg
     4021 cgtcccgacg ctacttcttg tcgaaccgct ttcccacctc gctccagttg accacgttcc
     4081 accacgccgc cacatagtcc ggccgacggt tctggtactt gagatagtag gcgtgctccc
     4141 agacgtcgca gccgagcacc ggcgtcttcc cgtccatgat cggactgtcc tgattgggcg
     4201 tactctcgat ggccagcttg ccgctcttgt ccagggtgag ccatccccag ccgctgccga
     4261 accggccgac gcaggccttg gcgaactgct ctttgaacga ggcgaagcct ccgagattct
     4321 tggtgatggc gtcggctagc gcgccggtcg gttctttcgg gccgcccggc cgcatgattt
     4381 cccagaagaa ggtgtgattg gagtgaccgc cggcgttgtt ccggaccgcg gtgcgaatcg
     4441 cttccggcac gctgttgatg ttccgcagca agtcgtcgag cgatttctgc tgcaggtccg
     4501 gatgggattc cagcgcggcg ttgagattgt tgacgtaggc ggcgtgatgc ttctcgtgat
     4561 ggatccgcat ggtctgctcg tcgatgtgcg gctccagtgc gttgtgcgga tacggcaggg
     4621 ggggaagggt gaaagccatg tcgaaccatc ctccgcgaac agcgtttacc gatcgtagtg
     4681 atcccgtttt ctagtcggcg caatatgact gtgcctcaca gagcggtcaa cgacgtccgt
     4741 caccggcaag tccgcgaatc gcctgcacgt accagcggac cgcgcggagg tagtcgccga
     4801 c
//
LOCUS       DASRJQ010000358         2514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2844377, whole genome shotgun sequence.
ACCESSION   DASRJQ010000358 DASRJQ010000000
VERSION     DASRJQ010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2844377"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /locus_tag="VFB89_07950"
     CDS             complement(<1..1296)
                     /locus_tag="VFB89_07950"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZM27275.1"
                     /translation="MQADAFLKDLALVLCVAAITSVVFQRLRLPAAPGYIVAGLLIGP
                     HVPFPLVANLETVQTLAELGVVLLMFSIGLEFSLRRLVRIGPRVGLVVLLEVGLMLAA
                     GSLVGRLFGLGTEASVLAGGVVAISSTMIVASVFAEARVEGPLRELVLSITVVEDLAA
                     ILLIAAATTLASGGSLTAGDLARTGGRLLAFLAGALAIGLLVVPSAMRAVARLRRPET
                     TLVASVGLCFAAALLARQAGYSVALGAFLAGSLVYESGAARLVEQLVRPLRDMFAAIF
                     FVAVGMLLDPAVAAANWPLIAALVAVLVCGKIIGVTVGSFLTGHGSRLSLRAGMSLAQ
                     TGEFAFIIVGVGAGIGGPARVLPHVAAAVSVITAFASPWLVRASEPLALYVDRRLPRP
                     LQTFATLYGSWIELLPGTRGRTTRWTPVRRAASFVLGDAV"
     gene            1382..1888
                     /locus_tag="VFB89_07955"
     CDS             1382..1888
                     /locus_tag="VFB89_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27276.1"
                     /translation="MTARSNTPPVLEPSAEAVKVARDRLREKGVNVSEELVRDALRGV
                     LAVEAGRIERRSREAAAACLLKVQEATREALAMLAQPNDATLSIPAVLAAAAPAAPPK
                     APAAKPSGPPPRRDTPANLADVVRRRLGDDAPDDSSTRPPSPPTPLPSPARPQEPRRP
                     VFKRPRGR"
     gene            2003..2266
                     /locus_tag="VFB89_07960"
     CDS             2003..2266
                     /locus_tag="VFB89_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27277.1"
                     /translation="MEGLLVTTGLYLCVALIVVGAYLAFPAEPKVTHRHWEGVHPGDT
                     VVVHGESRQVIAIDVAHDMVRLDRPFDFPPQSSDWVVVRHASA"
     gene            complement(2330..>2514)
                     /locus_tag="VFB89_07965"
     CDS             complement(2330..>2514)
                     /locus_tag="VFB89_07965"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HZM27278.1"
                     /translation="RCLSLIEMVTDRPGHDFRYAIDPSRLEREVGWRPKESFETGLRK
                     TVEWYLANRWCLNPNT"
BASE COUNT          479 a          872 c          820 g          343 t
ORIGIN      
        1 gacggcatct cccaggacga agctggcggc gcggcgcaca ggtgtccacc gcgtcgtcct
       61 gccgcgcgtg cccggcaaga gctcgatcca ggagccgtag agtgtcgcga aggtctgaag
      121 cgggcgcggg agccggcgat cgacgtagag cgcgagcggc tcggatgcac ggaccaacca
      181 cggagaggcg aacgcggtga tcactgagac ggctgctgcg acgtgtggaa ggacacgggc
      241 aggtccgcca atgccggcgc cgactccgac gatgatgaac gcgaactccc ctgtctgggc
      301 caggctcatg ccggcgcgga ggctgagccg cgacccgtgg ccggtgagaa atgaccccac
      361 cgttactccg atgatcttgc cgcacaccag caccgcaacc agcgcggcaa tcagcggcca
      421 gtttgcagca gcgaccgccg ggtcaagcaa catccccacg gcgacgaaga agatcgcagc
      481 gaacatgtct cgcagcgggc gaaccagctg ctcgaccagc cttgcggcac ccgactcgta
      541 caccagtgag cctgcgagga acgcacccag cgccactgaa tagcccgcct gccgggccag
      601 gagtgcggcg gcaaagcaga gccccacgct ggccaccagc gtcgtctcgg ggcgacgcag
      661 ccgggccacg gctcgcattg ccgagggcac caccagcagc ccgatggcca gagcgccggc
      721 caggaaggcg agcagccggc cgcccgtgcg cgccaggtcg ccggccgtta gggaaccacc
      781 cgaagccagc gtcgtggcgg cggcgatgag caggatggcg gcaaggtcct ccaccaccgt
      841 gatcgagaga accagctccc ggagcggacc ttcgacgcgg gcctccgcga atacgctcgc
      901 gacgatcatg gtactcgata tggccaccac gccacccgcc agcacactcg cctcggtgcc
      961 aagcccgaag agccgaccca ccaaagagcc cgcggccaac atgagcccga cttcgagcag
     1021 cacaaccagc cccacccttg gcccgatccg caccagccgg cgcaagctga actcgagccc
     1081 gatcgagaac atgaggagca cgaccccaag ctcggccagc gtctgcaccg tttcgagatt
     1141 ggcgaccagc ggaaacggca cgtggggtcc gatgagcagt ccggcgacga tgtagcccgg
     1201 agcggccgga agcctcagtc gctgaaagac caccgacgta atcgcggcga cacaaagcac
     1261 cagcgccagg tccttcaaga aggcatcggc ctgcatcaat actcgatcgg aagtggtggt
     1321 agcgaccgct gcgtgcccgc agggaatgga tatagtttac ggcatccaag gagggtatgt
     1381 catgacagca cgctcgaata caccccctgt cctggagccg agcgcggaag cggtgaaggt
     1441 cgcccgggat cgcttgcggg agaagggtgt gaacgtctcg gaagagctgg tccgcgatgc
     1501 cttgagaggc gtgctcgcgg tcgaggcagg tcggatcgag cgccggtcgc gggaggccgc
     1561 ggcggcctgc ctgctgaagg ttcaggaagc gacccgcgaa gcactcgcca tgctggctca
     1621 gcccaacgac gcgacgcttt caatcccggc agtcctggcg gcggccgctc cggccgcccc
     1681 tcccaaggca ccagcggcca aaccgtcagg accgcccccg cgccgtgaca cacccgccaa
     1741 tctcgccgat gtggtaaggc gacgcctggg cgacgacgct cccgacgact cctcaacgag
     1801 gccgccctcc cctcccaccc cgcttccctc accggcacgg ccgcaggagc cgcgccgacc
     1861 cgtcttcaag cgtccccgcg ggcgctagcg cccgcggcac cttccggggc ttttgctctt
     1921 gcggcggcgt cgccaggcgc cgcatgttcc aatgggggtt gcccccagcc gtcgtcactc
     1981 ttgagcgcag gaggatcgcg cgatggaagg acttctcgtt acgacggggc tttacctctg
     2041 cgtcgcgctc atcgtggtcg gggcctatct cgcgtttccg gccgagccca aagtcaccca
     2101 ccgccattgg gaaggagtgc atccgggaga taccgtcgta gtgcacggcg aatcacggca
     2161 ggtgatcgcc atcgatgtcg cccacgatat ggtgcgcctg gatcgcccgt tcgacttccc
     2221 gccccagtcc agtgactggg tagtggtgag acacgctagc gcctagcgag cagaggcgag
     2281 cactaaccag agatgaggcg gaccggggca ggccgctgcc ccggagacgt caggtattgg
     2341 ggttgaggca ccatcggttg gccaggtacc actctaccgt cttccgcaag cctgtttcga
     2401 agctctcctt cggacgccag ccaacttccc gctccagccg actcggatca attgcgtagc
     2461 ggaagtcgtg gccggggcgg tctgtcacca tctcgatcag gctgaggcag cgct
//
LOCUS       DASRJQ010000359         3453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3601540, whole genome shotgun sequence.
ACCESSION   DASRJQ010000359 DASRJQ010000000
VERSION     DASRJQ010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3601540"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..1592)
                     /locus_tag="VFB89_07970"
     CDS             complement(42..1592)
                     /locus_tag="VFB89_07970"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27279.1"
                     /translation="MSFPMAILVAGLSGFIALSYEILWYRVFAVAFGGTAQGFALLLG
                     FYLYGLAAGALVVERLCRRKQNWPTRRHLLALATFNLGAYVTAYLVIPFLGLGCRHSL
                     CLIALPAVAVSTTLLGAALPLISHLAVRPDARAGAGLSRVYFANILGSTLGSLLTGYV
                     LLDRMPASSVALVLAVLGLLLSAALFWAARGSVVVFAGLGLLCFGLVRLTPGLFDGLY
                     EKLIFKHQFRPDIRFAHTIENRVGVVNVTRTGKVFGGGVYDGWARIDLVHDPNLLVRA
                     VAIPVFHPRPRRALVIGLSMGAWAQLIANLPTIEQLTIVEINPGYLSLIPQYPDVASL
                     LTNPKVRVAIDDGRRWLARHPDEQFDLIVANITFHWREHATNLLSADFLRQVRTHLAS
                     GGVYFYNATSSPDVMKTGFTVFPYGLRFGSFIAVSDTPLRLDHAAWAAAIRDLTVDGR
                     LLLNPADPVQAAAAQRLTSVINDSVGPNANWEWRSSVLKDVAEARIVTDDNMAPEWSF
                     LAVGAWDP"
     gene            complement(1589..2239)
                     /locus_tag="VFB89_07975"
     CDS             complement(1589..2239)
                     /locus_tag="VFB89_07975"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HZM27280.1"
                     /translation="MTVATEPPPIRTRPEQTSRAGLLFPLFLLSGFAAILYQVVWQRT
                     LFAVVGINVEAVTLIVTTFIFGLGLGSLLGGRLSHGTPDQLVRRFAAIELAIGAFGLV
                     SLPLMRQVGEWTTSLTPLATGVVTFGVLLIPTLGMGATLPILVAHYVRRSGNVGTAVG
                     GLYFVNTLGSALAALTASYAMMRALGQSGVIWVAVSCNVVVATTVWTLMRDREEPA"
     gene            complement(2236..>3453)
                     /locus_tag="VFB89_07980"
     CDS             complement(2236..>3453)
                     /locus_tag="VFB89_07980"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZM27281.1"
                     /translation="QTLRRPTEHKAVTVSSPPEAEPQTVSPPDRSLGAGRIIRAVVVW
                     QLLGLVAWGLSPAPRFLIAALTGSALLLLLAEPARVRLLRAAILTFAFGLIWSVGPSG
                     SQVSIVSHPGLIGAFAGLGALADFALHPARDHRVNVAGFGVPAFVFVVALTLAPIGHG
                     WVVTQDAVLLLADRALGGLPSYAVADAFAAWPPLAAFAWVAYLTLPLEASLVLLVSIR
                     RERADMDSRRILLACAVAGLLGVLGYWLCPATGPRYAFPGFPVRPVGVSLGGISVAPD
                     FPRNAMPSLHFAWACILYRAARPVPWLHWTTLAWLIGIAVAALGFGEHYLIDLVVAVP
                     FLAAVESALIRRWRWMLVTVTVAAAWIIYLGRGGGLDPVVGWIAVTGSLVIGLLALPR
                     RVYGAPAVLEANA"
BASE COUNT          742 a         1068 c         1153 g          490 t
ORIGIN      
        1 ccggtcgaat gcgcgagacg gtggagatca gcacggaagg cctaagggtc ccacgctcct
       61 acggcgagaa agctccactc cggcgccatg ttgtcgtcgg tgacgatgcg ggcttcggcc
      121 acgtccttga ggacggagct ccgccattcc caattggcgt ttggaccgac gctgtcattg
      181 atcaccgacg tgagccgctg tgccgcagct gcctgaaccg ggtccgcagg gttcagcaat
      241 aggcgcccat cgaccgtgag gtccctgata gcggcggccc aggctgcgtg atctaagcgg
      301 agtggcgtgt cgctgaccgc gataaagctt ccgaaccgca agccatatgg aaagacagtg
      361 aaccccgttt tcatgacgtc cggagacgac gtcgcgttgt agaagtagac gccgcctgag
      421 gccagatgag tgcggacctg cctgagaaag tcggccgata acagattcgt ggcgtgctcg
      481 cgccaatgga atgtgatgtt ggcaacgatc aggtcgaatt gttcgtccgg gtgtcgcgcc
      541 agccagcggc ggccgtcgtc gatcgcgacg cggaccttcg ggttggtgag caggctcgct
      601 acgtccggat attgcgggat gagggacaaa tatcccggat tgatctccac aatggtcagt
      661 tgctcgatgg taggcaggtt ggcaatcagc tgagcccagg cgcccatcga cagcccgatt
      721 accagcgccc gtcggggccg cggatggaat acgggaatcg ccacggccct caccagcagg
      781 ttggggtcgt gaaccaggtc gatgcgtgcc cagccatcgt agactccgcc gccgaagact
      841 ttacccgtgc gggtgacgtt taccacgccg actcggttct cgatggtgtg cgcaaaccga
      901 atgtcgggac gaaactggtg cttgaaaatg agtttttcgt agaggccatc aaacaagccg
      961 ggtgtgaggc gtaccagacc gaagcagagg aggccgaggc ctgcgaagac aacgaccgat
     1021 cctcgtgccg cccagaacaa ggcggccgag agcagcagcc cgagcaccgc gagcacgagc
     1081 gcaaccgagc tggcaggcat ccggtccagc agcacgtacc cggtcaacag gctccccaga
     1141 gtggacccga gaatgttcgc gaagtacacc cgggagagtc cggcaccggc ccgcgcgtcg
     1201 gggcgaacgg ccaggtggct gattaatggc agggcggcac ccagcagcgt ggtggagacg
     1261 gcgaccgcgg ggagcgcaat gaggcagaga gaatgacggc agcccaatcc aagaaagggg
     1321 atcaccagat aggccgtgac gtaggcgccc aggttgaacg ttgccagcgc cagcagatga
     1381 cggcgagtag gccaattctg cttgcggcga cagagtcgct cgaccacgag ggctcccgcg
     1441 gccaagccgt acaggtagaa gcccaacagc agggcaaacc cctgtgccgt tccgccgaat
     1501 gcgacggcaa acacacgata ccataggatc tcgtacgaca gggcgatgaa cccgctcagg
     1561 ccggcgacca ggatcgccat cgggaagctc atgccggctc ctcccgatcc cgcatcagag
     1621 tccaaacggt cgtcgcaact accacgttgc acgagaccgc gacccagata accccggatt
     1681 gtcccagggc ccgcatcatc gcgtagctgg ctgtgagtgc ggccagtgcc gaaccgagag
     1741 tgttgacgaa gtacagcccg ccgacagccg tgccgacgtt gccggagcgt cgcacgtagt
     1801 gagcgacgag gatggggagc gttgcgccca tgcccagggt cggaatgagc agtacaccga
     1861 aggtgaccac tcccgtcgcc aagggagtga gcgacgtggt ccactcgccg acctggcgca
     1921 tgagcgggag ggaaaccagg ccgaacgctc caatggcgag ctcgatagcg gcgaagcggc
     1981 ggacaagctg gtccggcgta ccgtgcgata gccggccgcc cagcagactg ccgaggccca
     2041 ggccgaaaat gaatgtggtt acgatcagcg tcactgcctc gacgttgatg ccgacgacgg
     2101 cgaagagagt gcgctgccag actacctgat agagaatggc ggcgaagccg gacagaagaa
     2161 acagcggaaa cagaaggccg gctcggctcg tctgctctgg ccgtgtcctg attggaggcg
     2221 gttcggttgc gaccgtcatg cgttggcctc gagcacagca ggggctccgt acacccggcg
     2281 aggtagggcc aggaggccga tcacgaggct gcctgtaacg gcgatccacc cgactacggg
     2341 atcgagccct ccgccacggc ccagatatat gatccatgcg gcggcgaccg taacggttac
     2401 cagcatccat ctccaacgac ggatcagcgc cgactccaca gcggccagga acggaaccgc
     2461 gaccaccagg tcgatcaggt agtgctcacc gaatccgagc gcggcaaccg caatgccaat
     2521 gagccaggcc agagttgtcc agtgcagcca gggcaccgga cgcgcggcgc ggtacaggat
     2581 gcaggcccag gcaaaatgga gtgacggcat cgcgttgcgg ggaaagtcgg gggccacgct
     2641 gatcccgccg aggctgactc ccactggccg aactggaaag ccagggaatg cgtagcgtgg
     2701 gccggtcgcg gggcagagcc agtatccgag gacgcccagc aagcccgcga cggcgcatgc
     2761 gaggaggatc cggcgcgaat ccatgtcggc acgctctcgg cgaatcgaca cgagcagaac
     2821 cagcgaggct tcgagcggca gggtgaggta ggcaacccac gcgaaagccg cgagtggtgg
     2881 ccaggccgcg aatgcatcgg ccacggcgta gctcggcaat ccacccaggg cgcgatccgc
     2941 gagcagaagc actgcatcct gcgtcacgac ccagccatgc ccgatcgggg ccagggtaag
     3001 cgcgacgacg aacacgaacg cgggaacccc gaacccggcc acgttcactc gatggtcgcg
     3061 tgccggatgc aacgcaaagt cggccagtgc cccgagcccg gcgaacgctc cgatcaatcc
     3121 gggatgactc acgatactca cctgactgcc ggaaggtccc acgctccaga tcagcccgaa
     3181 ggcaaaagtc agaattgctg ctcgcagcag ccgcacccgc gccggctcgg ccagcaggag
     3241 cagcagcgcg ctgccagtga gggcggctat gagaaagcga ggggctgggc tcaatcccca
     3301 ggcgaccagt ccgagcagtt gccagaccac cactgctcga ataattcgac ccgctccgag
     3361 gcttcggtcc gggggagaga cagtctgcgg ctcggcttcg ggtggcgacg agacggtcac
     3421 tgctttgtgc tcggttggcc tacgcagcgt ttg
//
LOCUS       DASRJQ010000360         4319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1337186, whole genome shotgun sequence.
ACCESSION   DASRJQ010000360 DASRJQ010000000
VERSION     DASRJQ010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337186"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VFB89_07985"
     CDS             complement(<1..275)
                     /locus_tag="VFB89_07985"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HZM27282.1"
                     /translation="MSPIAESPLSNKDLAPTGPAQRTWGTYNLAALWIGLSIVITTYT
                     LASGLIVAGMEWWQGLLTVALGNLIVLIPILLNSHPGTKYGIPFPVLV"
     gene            complement(371..1027)
                     /locus_tag="VFB89_07990"
     CDS             complement(371..1027)
                     /locus_tag="VFB89_07990"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HZM27283.1"
                     /translation="MDAPSRKRAAIADPEAREFLRRADRVLAQLIDARPDFRPRAWMD
                     ELPPLDAFGTLVFQVAGQQLSVSSTRAIISHLQERFGGHLPSPAELLAADPQVLRDSG
                     FSARKGQTLRALAERFVDGRLSDEALSRMTDDEVETTLTEVPGIGLWTARGFLLVALD
                     RPDVFLSGDLALRRAIQRAYGFDHLPTEDEVAQVSDRWRPYRSLGVSYLFASEYDGQP
                     "
     gene            complement(1030..1203)
                     /locus_tag="VFB89_07995"
     CDS             complement(1030..1203)
                     /locus_tag="VFB89_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27284.1"
                     /translation="MTVSTDMDVRVEDVRERSRFEVRVGDDLARFARYRRRPGLIAFI
                     HTRSVLTDRLVRA"
     gene            1541..2482
                     /locus_tag="VFB89_08000"
     CDS             1541..2482
                     /locus_tag="VFB89_08000"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZM27285.1"
                     /translation="MTERISLEGKVAVVTGAGRGLGRAYVELLAERGARVVVNDLGTD
                     VSGFGKDSTIAEQVADLIRSRGGEAIANGSDVSTPEGGSDLIATTIEHFGRTDLLVNN
                     AGICGSQLFEDATLDDFDHYWRVHLGGPVNTVKAAWPHMVAQRSGKIILTTSVAGLFG
                     IRGQATYAAAKCAVVGLMRILAIEGAEHGILVNTISPSAYTRMHPAATGSGSVFGRGP
                     DPAAWLKQSEATMPVEAVAPAIVWLASDRCSETNSIYNVEAGAIQRIAIVTGPGFHDP
                     HLTPESIAENYAKIASIEGFSEPGPFEPGPHRPLGGT"
     gene            2684..4174
                     /locus_tag="VFB89_08005"
     CDS             2684..4174
                     /locus_tag="VFB89_08005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZM27286.1"
                     /translation="MLIPSGELRFGSAAGRWVIAASVLGSGIAAIDATVVGVALPAIG
                     REFHIGVAALQWVVSGYFLTLASFLLLSGSLSDRFGRKRLFGIGVVGFAVASAACGLA
                     PSAGFLIAMRVVQGIGGALLVTGSLAILEASFVPSDRGAAIGAWAGLGAVATAVGPLL
                     GGYLISAASWRWIFFLNVPVAVVVLFVAVRHVPESKDPSAAHHIDVAGASLAVVGLGA
                     VAFALIEGPGLGWSSIEVVAALALAIGALGTFAFVELRSPQPLLPLGMFRQRQFSSTN
                     AITFLMFASVGGAFFLLPVQLQIGSGYSPLQAGAALLPVTIIELVFSVRSGRLASRIG
                     PRLQLIAGPFIFGASLVLLTRITDGRGYVVYVLPAMIVMGIGVATFVAPLTATVMQSA
                     APEHAGLASAINNDVARIGSLIAVSVLPAIAGITGTTYLHPAELSAGFRTAMFVSAVG
                     CGLAGILATLTITNPERQAEPSTPYAHCAIDAPPLRVSIPEVSRED"
BASE COUNT          720 a         1399 c         1399 g          801 t
ORIGIN      
        1 accagcaccg ggaacgggat cccgtacttc gtgcccgggt gcgagttgag caggatcggg
       61 atcagcacga tgaggttgcc gagcgcgacg gtgagaagcc cctgccacca ctccatgccc
      121 gccacgatca gcccggaggc cagcgtgtag gtggtgatca cgatcgacag cccgatccac
      181 agcgcggcga ggttgtacgt cccccacgtg cgctgagcgg gcccggtcgg cgcgaggtcc
      241 ttgttcgaca ggggcgactc ggcgatgggg ctcacgtgag tcgatcctcc gcgatcggcc
      301 gtttctggac aagaggggaa cccccactgt tttcccctgc ccttcgctga gcctctacag
      361 gtcggccggc ttagggttgc ccgtcgtatt cggacgcgaa caggtagctc accccaaggc
      421 tccgatacgg ccgccagcga tccgacacct gagccacctc gtcttcggtc ggaagatgat
      481 cgaagccgta cgcccgttgt atcgcgcgcc gcagcgcgag gtcgcccgaa agaaagacgt
      541 ctggccgatc gagagccacg agcaggaagc cacgcgcggt ccacagcccg attccgggga
      601 cctcggtcag ggttgtctcg acctcgtcgt cggtcatgcg cgagagcgcc tcgtcgctga
      661 ggcgtccatc gacgaacctc tccgcgagcg cgcgcaaggt ctggcccttt cgagccgaga
      721 agccgctatc gcggagcacc tgcggatcag ctgcgagcag ctcggccggc gacggaagat
      781 gcccaccgaa ccgctcctgt agatgcgaga tgatcgcccg ggtcgagctg accgagagct
      841 gttggcccgc gacttggaag acgagcgtcc cgaaggcatc gagcggagga agctcgtcca
      901 tccaggcccg cggccggaag tccggccgcg cgtcgatcag ctgcgcgagc acccggtccg
      961 cccttcgaag gaactcgcgc gcctcaggat cggcaatcgc ggctcgcttt cgcgatggag
     1021 cgtccatgct cacgctctta ccagccggtc ggtgagcacg ctgcgtgtat ggatgaaggc
     1081 gatcaggccg ggtcgtcgcc gatacctggc aaaacgtgcg aggtcgtccc caactcgcac
     1141 ttcgaaacgc gaacgctcgc ggacatcctc gacgcgcacg tccatatcgg ttgaaaccgt
     1201 catcgggaaa ccagccttcc tggcgttctc cgcagactca atcacgtact cgagaggtca
     1261 cgaacagcct cgccctcgac gtccgaacac gcctcggcca agttctcacg cacatggtgc
     1321 acctaccacc atcatgaggc ttaccgccct cggtggcatc caggaaaccc acagtcgccc
     1381 gctcggcccg cgcagcggct cccggcgccg cttacccggc gtgagggagc gcgctctggt
     1441 tgccagcggt caccaaatct tgcggtggtg ttggcacctc gagggccgcc catccagact
     1501 tcgacggttg agcctggcct gctcggaaga ggatggcgag atgactgaac gaatttcgtt
     1561 ggaggggaag gtcgcggttg tgactggggc aggccgcggg ctggggcggg catacgttga
     1621 acttctcgcc gaacggggtg cccgggtcgt ggtgaacgac ctggggaccg acgtatcggg
     1681 gttcgggaag gactcgacga tcgcggaaca ggtggccgac ctcattcggt cacgtggagg
     1741 cgaggcaatc gcgaatggca gcgatgtttc cacccccgaa ggtggcagtg acctgatcgc
     1801 gacgaccatc gagcatttcg ggagaacaga ccttctcgtg aacaacgctg gtatctgcgg
     1861 aagccagctc ttcgaagacg ccacccttga cgatttcgat cactattggc gcgtgcacct
     1921 cggcgggccg gtcaacacgg tgaaggctgc ctggccgcat atggtcgcac agcgctctgg
     1981 gaagatcatc ctcacgacgt cggtcgccgg cctgttcgga atacgtggcc aagccaccta
     2041 tgcggcggcc aagtgcgcag tcgtcggatt gatgcgcatc cttgcgattg aaggtgctga
     2101 gcatgggatt ctcgtgaaca ccatttcgcc aagcgcgtac acgaggatgc acccggccgc
     2161 gacgggcagt ggatcggtct tcggccgcgg cccagatccc gctgcttggc tgaagcagtc
     2221 ggaagccacc atgccggtgg aggctgtggc gccagcgatc gtctggctgg ccagcgatcg
     2281 ttgttcggag acgaacagca tctacaacgt ggaggcagga gcaatccaac gcatcgccat
     2341 cgtcacggga cctggcttcc acgatccaca tctgacaccc gagagcatcg ccgagaacta
     2401 cgcaaagatc gcatcgatcg agggcttctc cgaaccgggt ccgttcgagc ccggtccgca
     2461 tcgccctctt ggcggtacgt agatacggat acctgcgact ggctagctgg tgagatccga
     2521 acacacgcgg gttgcaaggc tgcgctacga ccggagtggg gctggacgcc ataggaccgt
     2581 gtcggggtga ccgcctcgtg ctcgggcatc gccgatcaat gccggcgagg gagttccagg
     2641 cgagcgggaa gaacgccaac gccgggcggg ccaaggagtg caagtgctga tcccaagtgg
     2701 agagctccgg ttcggttctg cggctgggcg ttgggtgatc gcggcgtcgg tgttgggctc
     2761 ggggatcgcc gcgatcgatg ccacggtggt tggtgtcgcg ctgccggcga tcggtcgcga
     2821 atttcacatc ggtgtggccg cgctccaatg ggttgtgagt ggttactttc tcaccctcgc
     2881 gtcgttcttg ctcctgagcg gttcgctgag cgaccgcttc ggcagaaagc gcctgttcgg
     2941 tatcggagtt gtcggctttg ccgtcgcgtc cgcggcttgt ggcctagccc cgagtgctgg
     3001 gttccttatc gctatgcgcg tggtgcaagg aatcggtggt gcactcctgg tcactgggag
     3061 cctcgccatc ctcgaggcgt cgtttgttcc gagcgaccgg ggcgcagcga tcggggcgtg
     3121 ggcgggtctc ggtgcggtgg ccacggcggt cggaccgctg ctgggcggat atctgatctc
     3181 ggcggcttcg tggcgttgga tcttcttcct caacgtcccc gtcgctgtgg tcgtgctctt
     3241 cgtcgccgtc cgccacgttc ccgagtcaaa ggatccctcc gccgcgcatc acatcgacgt
     3301 ggccggcgcg agtttggccg tcgtcgggct cggcgcggtc gcgttcgcgc tgatcgaagg
     3361 gccggggctc gggtggtctt ccatcgaggt cgtcgccgct ctggcgttgg cgatcggggc
     3421 cttggggact ttcgccttcg tcgagctgcg gagcccgcag cccctgctcc ctctgggaat
     3481 gtttcgccag cgccagttca gctcgaccaa cgcgatcaca ttcctgatgt tcgcgagtgt
     3541 cgggggtgcg tttttcctgc tccctgtaca actccaaatc ggctcgggtt actcgcccct
     3601 ccaggctggt gcggcacttc ttcccgtcac cattatcgag ctcgtctttt cggttcgctc
     3661 gggacgtctg gcatccagga tcggaccccg actgcagctg attgccggac ccttcatctt
     3721 cggtgccagt ctcgtgttac tcacccgaat cactgacggt cgcggctatg tcgtctatgt
     3781 cctgccggca atgatcgtga tggggatcgg cgtggcaacc ttcgtcgcgc ccctcaccgc
     3841 caccgtgatg cagtccgcag cgccagaaca cgcgggcctg gcctccgcca tcaacaacga
     3901 cgtcgcccga atcggcagct tgatcgccgt gtccgtgctc cccgcgatcg ccggcatcac
     3961 cgggaccacc tacctgcacc cagccgagct ctcggctgga ttccgtaccg ccatgttcgt
     4021 gtcagccgtc ggatgtggac tggccgggat cctggccacg ctgaccatca cgaatccaga
     4081 gcgccaagct gaaccctcaa ccccgtacgc gcactgcgcg atcgatgcac cgcccctccg
     4141 agtcagcatc ccagaggtgt cccgcgaaga ctgaccgtgc accgcacaga cggacagtgg
     4201 agtggacacg ttttccaacc ctcgaagagg tccctcggtc ggtgggcgag gggggacttg
     4261 aacccccaca tcctttcgga cacaggcacc tgaagcctgc gcgtctgcca attccgcca
//
LOCUS       DASRJQ010000361        12611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_65713, whole genome shotgun sequence.
ACCESSION   DASRJQ010000361 DASRJQ010000000
VERSION     DASRJQ010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 12611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12611
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65713"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /gene="argB"
                     /locus_tag="VFB89_08010"
     CDS             complement(<1..812)
                     /gene="argB"
                     /locus_tag="VFB89_08010"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HZM27287.1"
                     /translation="MSIDTTKGIAGLKGALRYVRAYRDHVFVVKLGGDVLSDPDVRDQ
                     VAAQLALLSSLSIRLVVVHGGGPQATALSHRLGQEPRIVAGRRVTDDGALEIAKMVYA
                     GQLNVDLLAALREHKVQAVGLSGIDADLLTAHRRPPVSVVDDNGRTSEVDYGHVGDID
                     RVDPRVLVTLMEARFVPVVASLAGGEDGEVFNVNADTVAESLAVALRAQKLIFLTGAP
                     GVLRDRNDPSTLVTFADPDDLAGLMASGSLSGGMRPKVEACIRAATGGVERT"
     gene            complement(898..1917)
                     /locus_tag="VFB89_08015"
     CDS             complement(898..1917)
                     /locus_tag="VFB89_08015"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF003384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HZM27288.1"
                     /translation="MTKRDFLAMEDWSAEAIEGLFALAGRIKRGEITGGLERKVLAMV
                     FMDPSLRTRTSFETAMFLHGGHAIVLEPGKGSWSLEIEPGAVMDGSTVEHIIEAARVL
                     SRYADAIAVRAFPRGAEWQVARRDEVIRNFARYSDRPVINLESTRRHPCQGLADALTL
                     REKLGETEGKRFVLTWAWHPRALPTAVPASAAIAAASLGMEVVIARPDGYEMDPEDTA
                     LIRRITQERGGEFLHIINDPDEALVGADAVYAKSWGAVRLFGKPDEEREARASLRDWR
                     VTEQRVRSTRGGKGIVMHCLPVRRNVEIDDTVLDGPNSVVIDQAENRLHVQRALLLEL
                     IGKTV"
     gene            complement(1914..3056)
                     /locus_tag="VFB89_08020"
     CDS             complement(1914..3056)
                     /locus_tag="VFB89_08020"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZM27289.1"
                     /translation="MDRPVSLLPVYAQMPVRPVSGRGSWLIDADGHKWLDAYGGHAVA
                     STGHSHPRVVRAIAEQAAQLLFYSTAVPHPNRERLADRLTALCPAPLEQVFFCNSGAE
                     ANENALGLARRHTGRRLIVSLRGGWHGRTAATLAVTDGVKYEEAAARAGVPLSRKVAV
                     DDVADLEASVDDSVAAVILEPVQGLSGARDLSVEFVRAARRVCDRHAALLIFDEIQCG
                     VGRCGAFTAAQAYGVTPDLLTMAKGLASGLPIGAVITTRSVGDRLHIGDLGSTFGGGP
                     VPCAAALATLDVIEEEGLVANAMAVGDYLKAGALALGIPGVTGRGLLIGLRLGRAAVP
                     VQRALFDRRVLTGTASDPDVLRLMPPLSFDRQEADLLLTALHEVLE"
     gene            complement(3056..4081)
                     /gene="argC"
                     /locus_tag="VFB89_08025"
     CDS             complement(3056..4081)
                     /gene="argC"
                     /locus_tag="VFB89_08025"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HZM27290.1"
                     /translation="MKVAVIGAAGYTGGELLRLLFQHPQVVECVATSRSQAGKPIAEI
                     HPALATLSDARFSGQGPGEVARGRDVVFLCLEHGESSRVAGEVLDAGPGLLIDLAADF
                     RTADLRLYERYYGAHSAPELAHCFTYGLADVLAQQLRGAKAIAAPGCFATAAALALYP
                     LARVELSSPPALFAVTGSSGAGVQPRSTTHHPMRAHNLFAYAVLGHRHEAELLMRWRE
                     WTGQAEATVRLMTHSGPFVRGIYLTLHARAAGSDVAVVDLFRRAYVGRPFVRILDQPP
                     ELTHAVGTNYALIHPAESGDRSEVQVMVAIDNLVKGAGGQAIQAMNLALGLPETDGLL
                     GGGMYPC"
     gene            complement(4078..5163)
                     /locus_tag="VFB89_08030"
     CDS             complement(4078..5163)
                     /locus_tag="VFB89_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27291.1"
                     /translation="MVVTTRVKLDRISSSTRNAHLGTEVIVGDQIVAAEGYVLAVRIL
                     HDKSTYNTIEDVGGRMVSLRAGDVLAGTLGTRRALRGYAGVVPPTIAPGDTLEVLNLG
                     GILGRCTSENPEIGPPFRAEVLGAILTFPELGDRVGRPAHIRDLAVPPADRLDSDVPV
                     VYVVGTCMNAGKTVAATELVRGLARSGLRVGAAKLTGVSLMRDALSMQDAGAIAALTF
                     NDVGVASTHAGVTVSTAKGIFNRLAQGKPDVIVAELGDGILGEYGVQDILRDAELMRA
                     GAAFVMAAPDPVACWGAAELMHREYGLPITAITGPATDNEVGNAYISAELRLAAHNAR
                     LDPAGLVGAVLTALKAWALGPVSPASR"
     gene            complement(5195..6385)
                     /locus_tag="VFB89_08035"
     CDS             complement(5195..6385)
                     /locus_tag="VFB89_08035"
                     /inference="COORDINATES: protein motif:HMM:NF044952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /protein_id="HZM27292.1"
                     /translation="MRNGTIALAFSGGLDTSFCVPRLAADGWSVHTVYVNTGGTTGQA
                     RAAIAAQARAVGALTHHEIDARTAVFDRFVRFLIQGNVLRGEVYPLSVAAERTQQALS
                     VIDTARAIGAAAVAHGSTGAGNDQVRFDVALRVLAPDLEIVTPIRDLGITREQAVAYL
                     TERRLPVPPQATAYSVNRGLWGTTWGGGWTHDTWAGPPAELLDPPAEAPSPREVVLAW
                     RCGLPVALDGEQLDGATLIEQLNDLAETYGFGRGIHVGETALGIKGRIGFEAGAALLL
                     IAAHRELEKLVLTKWQAFWKDQLARFYGDRLHEGQYFDPALRDIEALITSSQERVTGD
                     TRVRLSSGRFQVVGTRSPHSMMDRSVATYGEENRLWTGDEARSYARVAAIPALLAARA
                     GGKD"
     gene            complement(6453..8909)
                     /locus_tag="VFB89_08040"
     CDS             complement(6453..8909)
                     /locus_tag="VFB89_08040"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZM27293.1"
                     /translation="MNRSRRMPSLTGAPFALLLALASCQRPQAEEVGLPSEPAASHRL
                     PTGARLDPAGRSLAVGNMPLAARVSPDGRYLVLSLSGWREQGIEILDRTRGVVVQRLP
                     QPGAFLGLAWAADGSILYASGGSAEMVYLYTWDPARPEPAFLSDSITLRADSSDTTVH
                     RYPAGLALSRDGRVLYVAENLADSIAILDLATHRVRQRLGMSSYPYDVAVAPDDRVYV
                     SAWGGSTIGVFRATPDGLRADGSIEVGRHPSALCLSADGSRLFTTSASTDQIAVVDTR
                     AGRVIARLTDPPPSGVLEGSTPNALTLSLDGTRLLVAEADANAVAVFDLSAGVSGISS
                     AHGNDRLAGRIPTEWYPTALVLLDDSLWAVNGKGGGAGPNPRGAQPDTPLAKGDSLYT
                     LGQLNGTVTILPAKAAGRALDSLSARVAHANGWDASPSRQAHYPPFHHVIYVIKENRT
                     FDQVLSDLPRADGDTSLLFFPRNMSPNHHALAERFGIYDRFFVNAEVSAQGHPWSTAG
                     YVTDYVEKTTPDIYRLRRPETPGEGEVDDPAMGFLWNQAIKKGLALRIYGEYGEPIPP
                     QPDDPGPARYRSTRAALRPYTSDQYPSFDLGVPDQRRADVWVAEFQADVSAGRMPTLE
                     IIHLPSDHTAGARAGYPTPKAYMADNDLALGRIVDALSHSPFWASTVMFVLEDDAQDG
                     PDHVDSHRSVLLVISPYSRAGLVRRFVNTTDVLATIEEILDLGPLSQFDRFGRPLREI
                     WAGSPDLRPYAALTPTQSLTEINPSRGAGARRSARLDFSAVDRVEDDEFNRILWRALK
                     GSAPYPALRRAGTLEYTRSR"
     gene            9152..9670
                     /locus_tag="VFB89_08045"
     CDS             9152..9670
                     /locus_tag="VFB89_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27294.1"
                     /translation="MPTAFSPGSQSGSGLLPGYVALGCWQRGGAVLDERLELLIGQLS
                     AELSGCRWCIEQGRHRWRKALLPVVLLQQLRDQYTSALFSERDRAALMLTEAVARYTG
                     RVAAAADQAVARARCHFTESEIARIAHLAAVEHFYDPATGAVGQDVDELNCKAVMPSP
                     AIAPGFRTRGQP"
     gene            9667..10674
                     /locus_tag="VFB89_08050"
     CDS             9667..10674
                     /locus_tag="VFB89_08050"
                     /inference="COORDINATES: protein motif:HMM:NF042524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HZM27295.1"
                     /translation="MSPAPTPGRRWLLLIHQLPPKPDYLRVKIWRRLQRIGAVALKNS
                     VYVLPRTDQTVEHFQWILREIESSGGEASVCDAAFVTGLSDGQIETLFRAAREADYAA
                     LSEEVRALSRGTIARRGADMGRTDVEATLTRLRRRLQEIGAIDFFGAPGRQIAEAAIE
                     RLESQIRSTGRKSGASIGKTKGKARTGRIWVTRRGVHVDRIASAWLISRFVDPKARFR
                     FVEEQPYQPKGDEVRFDMFEGEYTHEGDRCTFETLIARFELTQPGLQALAEMVHDIDL
                     ADGKFGRAETAGLERLIDGITRAHVEDDERIAHSAILFDDFLRSFQHETGPKKRSARS
                     P"
     gene            10692..11417
                     /locus_tag="VFB89_08055"
     CDS             10692..11417
                     /locus_tag="VFB89_08055"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HZM27296.1"
                     /translation="MVNRLREARRVTVLTGAGISAESGVATFRDALTGLWAQYRAEDL
                     ATPEAFARNPALVWDWYRMRRQMVARAEPNAGHLALVEMEAQIPEFVLITQNVDGLHQ
                     RAGSSRPIELHGNITRARCSREDTPVDHWDETADSPPRCLACGAFLRPDVVWFGELLP
                     LEALRAAEMAARHCDVFLSVGTSNLVEPAASLPWTAAAAGAEVAVLNVSMDGQRRGPA
                     IEHLVGPAGELLPALVQATWPKR"
     gene            complement(11422..12513)
                     /locus_tag="VFB89_08060"
     CDS             complement(11422..12513)
                     /locus_tag="VFB89_08060"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZM27297.1"
                     /translation="MRVLLLLGLVAGFAGRLTAQQSDRVGQAQAVLAPLVESYGVSGA
                     EAPVRATVERLLPQWAKKKAETDTAGNLWVRVGKGDPLIVFVAHLDEIGFEIKAIRED
                     GSLEIETRGGFFPSLFEAQPALIHTGTGIVPGVFMPRDSVGSKPERAPAGFRVDVGTD
                     TRAATESLGVRVGHTITMPKHYVRLAGTRATGRSFDDRVGCAALILALRRLNPERLRH
                     EVAFIFSVREEIGLEGAAAAAASLGGTPVRVHAIDTFVSADSPLEPQTFAVAPIGRGP
                     VARALDNNSVAPPALVDSLARLARVNGIQLQIGTTNGGNDGAAFAAWGVPDVAIGWAL
                     RYSHSPAEVIDLRDVIGLGDMVQAIAERW"
BASE COUNT         2242 a         4187 c         4117 g         2065 t
ORIGIN      
        1 tgggtgcgct cgacaccgcc tgtggccgcg cggatgcagg cctccacctt gggccgcatg
       61 ccgcccgaca gcgagccgct cgccatcagc cccgccaggt cgtcgggatc cgcgaaggtg
      121 accagcgtcg aaggatcgtt ccggtcccgc agcacacccg gggccccggt gaggaagatg
      181 agcttctgcg cccggagcgc gacggccagc gattcggcca ccgtgtcggc gttcacgttg
      241 aacacctcac cgtcctcgcc tccggccagg ctggcgacga ccgggacgaa ccgcgcttcc
      301 atgagggtca cgagcacgcg cgggtccacc cggtcgatgt cgccgacgtg gccatagtcc
      361 acctcgctgg ttcgcccgtt gtcgtccacc accgagaccg gaggccggcg atgcgcggtg
      421 agaaggtcgg catcgatccc ggagagtccc accgcctgca ctttgtgctc cctgagcgcg
      481 gcgaggaggt ccacgttcag ctggccggcg tagaccatct tggcgatctc gagcgcgcca
      541 tcgtcggtga ctcgccgtcc tgcgacgatc ctcggctcct gcccgagccg gtggctcaag
      601 gcagttgctt gcggcccgcc cccgtggacc acgaccaggc gaatgctgag cgacgacagc
      661 aaggcgagct gcgcagctac ctggtcgcgg acatccggat cgctcaatac gtccccaccg
      721 agcttgacca cgaacacgtg gtcgcgatag gcgcggacgt agcggagggc ccctttgaga
      781 cccgcgatgc ccttggtggt gtcgatcgac atgcttctcc cgacagcgtg aacggtgaac
      841 ggtaaacggt gaacgggggt ggccgggcgg ggccgttcac cgttcaccgc ttaccgttca
      901 cacagtcttg ccaatcagct ccaacaacaa cgccctctgc acatgcagcc ggttctcggc
      961 ctggtcgatc accaccgagt tgggaccgtc gagcaccgtg tcgtcgattt cgacgttgcg
     1021 gcgcaccggc aggcagtgca tcacgatgcc cttcccccca cgcgtagagc gaacccgctg
     1081 ctcggttacc cgccagtcgc ggagagaggc gcgcgcctca cgttcttcat ccggcttgcc
     1141 gaacagacgg accgcacccc atgacttggc gtagacggcg tcggcgccga ccagcgcctc
     1201 gtccggatcg ttgatgatgt ggaggaactc gcccccacgc tcctgggtga tgcggcgaat
     1261 gagcgccgta tcttccggat ccatctcata gccgtctggg cgtgcgatca ccacttccat
     1321 tccgagagac gccgctgcga tcgcggcgct ggcggggacg gccgtgggca gggcgcgcgg
     1381 atgccaggcc caggtgagca cgaatcgctt gccctcggtt tcgccgagct tttcgcgcag
     1441 cgtcagcgca tccgccaacc cttggcaggg gtgccggcgg gtggattcga ggttgatgac
     1501 cggccgatcg gagtagcgcg caaagttccg gatgacctcg tcgcgccggg cgacctgcca
     1561 ttcggccccc cgagggaagg cgcgcaccgc gatcgcatcg gcgtaacgcg acaacaccct
     1621 tgcggcctcg atgatgtgct cgaccgtcga gccatccatc accgcgcctg gttcgatctc
     1681 gaggctccaa ctccccttcc ccggctcgag gacgatggcg tgaccgccgt gcaggaacat
     1741 cgcggtctcg aagctggtcc gggttctgag gctcgggtcc atgaagacca tagcgagtac
     1801 cttgcgctcg agcccaccgg tgatctcacc tcgtttgatc cgcccggcca acgcgaagag
     1861 gccttcgata gcctccgcgc tccagtcctc catggccagg aaatcgcgtt tggtcactcc
     1921 agaacctcgt gcagcgcagt cagcagtagg tcggcctctt gccggtcaaa cgacagcggc
     1981 ggcatcagac gaagaacatc cggatcactt gcagtccccg tgagcacccg ccgatcgaag
     2041 agggctcgct gaaccgggac ggccgcccgc cccaggcgaa gcccgatcag aagccctcgt
     2101 ccggtcacgc cgggaatccc cagggcgagc gcccctgcct tcaagtagtc gccgaccgcc
     2161 atcgcattgg cgacgaggcc ctcctcttcg atcacatcca gcgtggccag agccgcggca
     2221 cacggcaccg gtccgccgcc gaaggtactg ccgagatcgc caatgtgaag ccggtcgccc
     2281 acggagcggg tcgtgatgac cgctccgatc gggaggccgg aggcaagacc cttggccatc
     2341 gtgagaagat ccggcgtgac gccgtaggct tgcgcagccg taaatgctcc gcaccggccg
     2401 accccgcatt gaatctcgtc gaagatgagc agcgcagcgt ggcggtcgca gacccggcgg
     2461 gcggcccgca caaactccac gctcaagtcg cgcgcgccgg acaaaccctg caccggctcg
     2521 aggatcaccg cagcgaccga gtcatctacc gatgcctcca ggtcggcgac gtcgtcgacc
     2581 gcaaccttac gcgatagcgg gacgccggct cgagcggcgg cttcctcgta cttgacgcca
     2641 tcggtgacgg cgagcgtcgc ggctgtgcgc ccatgccagc caccccgcag cgagacaatg
     2701 agcctgcggc cggtgtgccg gcgcgcgagg cccaacgcat tctcattcgc ctctgctccg
     2761 gaattgcaga agaatacctg ctccagggga gcgggacaga gcgccgtgag gcggtccgcc
     2821 aggcgctccc gattcggatg aggaacggcg gtggagtaaa acaggagttg agcggcctgc
     2881 tccgcgatgg ctcgaacaac cctgggatgg ctgtgcccag tcgaggcaac cgcgtggcca
     2941 ccgtaggcgt cgagccattt atggccatct gcatcaatga gccaggagcc ccggccggag
     3001 accggacgca ccggcatctg ggcatacacc ggcagcagcg aaactggccg gtccatcagc
     3061 atggatacat tcccccaccc aagagtccgt cggtttccgg aagtcccagc gcaagattca
     3121 tcgcctggat tgcctggcct ccggccccct tcacgaggtt gtcgatcgcc accattacct
     3181 gcacttcgct acggtcgccg gactcggccg gatgtatgag ggcgtagttc gtccctacgg
     3241 cgtgcgtgag ctcgggaggt tgatccagaa ttctgacgaa ggggcggccc acgtaagcgc
     3301 ggcggaataa atcaaccacg gctacatcgc tgccggcggc tcgcgcatgc agggtgagat
     3361 agatccctcg aacgaacggg ccggaatggg tcatgagccg cactgttgcc tccgcttgcc
     3421 cggtccactc ccgccaacgc atcagcagct cggcttcatg gcgatggccg agcaccgcgt
     3481 atgcgaacag gttgtgcgct cgcatgggat ggtgcgtggt cgaccggggt tgaacgccgg
     3541 cgccactgga tcccgtcacg gcgaagagcg caggcggcga actgagctca acgcgggcaa
     3601 gcggatacaa cgcgagggcc gctgcggtgg cgaagcatcc gggcgcagcg atggctttag
     3661 caccgcggag ctgctgcgcc agcacgtcgg ccagcccata ggtgaagcag tgggccagct
     3721 ctggcgcaga gtgggcaccg tagtatcgct cgtaaaggcg gaggtcggcg gtccggaagt
     3781 cggcggcaag atcgatgagc agtccggggc cggcgtcgag cacctccccg gcaacccggg
     3841 acgattcccc gtgctccagg cacaggaaca ccacgtcgcg accgcgggcc acttcgcccg
     3901 ggccctgccc ggaaaaccgc gcatcgctaa gagtcgccag cgcgggatgg atctccgcga
     3961 ttggcttgcc ggcctggctc cggctggtcg cgacgcactc gactacctgc gggtgctgaa
     4021 acaggagacg cagcagctcg ccgcccgtgt atccggcggc accgatgacg gcgaccttca
     4081 tcgggaggct ggtgacacgg ggcccagcgc ccacgccttt aacgccgtca gaacggcgcc
     4141 gaccaatccc gccggatcga gccgcgcgtt gtgggcggcg agcctcagct cggcgctgat
     4201 gtacgcgttg ccgacctcgt tgtcggtggc cgggcctgtg atcgcggtga tcggcagacc
     4261 gtattcacga tgcatcagct ccgctgcacc ccagcacgcc accggatcag gcgccgccat
     4321 cacaaaggcc gcacctgccc gcatgagctc ggcatcgcgg aggatgtcct gcaccccgta
     4381 ttcgccgaga ataccgtcac caagctcggc gacgatcaca tccggcttgc cctgcgccag
     4441 ccggttgaaa attcctttcg cggtagaaac ggtcacgccg gcgtgggtgc tcgccactcc
     4501 gacgtcgttg aatgtgagcg cagcaatcgc gccggcatcc tgcatcgaga gagcatcgcg
     4561 cataagagag acgccggtga gcttggccgc gccgacgcga agtccggatc gtgccaggcc
     4621 gcgcaccagc tccgtggccg cgacggtctt gccggcattc atgcaggtgc cgacgacata
     4681 gaccacgggc acgtcactat cgagccgatc ggccggagga acggcgaggt cacgaatgtg
     4741 agccggccgg cccacccgat cacccagctc cgggaaggtc aggatcgcgc ccaacacctc
     4801 agcgcgaaac ggtggaccaa tctccgggtt ctcggacgta catcggccca ggatgccgcc
     4861 caggttcagg acctcgagtg tatcgcccgg cgcgatcgtc ggagggacga ctcccgcgta
     4921 gccccggagt gcacgacgag ttcccaacgt tccggccagc acatcgccgg ctcggagcga
     4981 aaccatccga ccgccgacgt cttcgatggt gttgtacgtc gatttgtcat gcaggatccg
     5041 cacggcgagc acgtaccctt cggcagcgac aatctggtcg ccgacgatga cctcggtccc
     5101 gagatgcgca ttccgggtcg aagacgagat ccggtccagc ttcactcgtg tggtcaccac
     5161 catctgcggc tcctgttcga aaagcgcaac ggcgtcagtc cttgccaccg gcgcgcgcgg
     5221 ccagcagcgc cggaatcgcc gccaccctgg cgtacgaccg agcttcatcg ccggtccaga
     5281 gccggttttc ctccccgtag gtcgcaacgc ttcgatccat catcgaatgc ggactccgcg
     5341 tccccaccac ctgaaagcgc ccggaagaca gccgcacgcg ggtgtcgccg gtaacccgct
     5401 cctgcgagct ggtaatcagt gcctcgatgt cacgcagcgc agggtcgaag tactgtccct
     5461 catgcagccg gtcaccgtag aaccgggcga gctggtcttt ccagaacgcc tgccatttgg
     5521 tgagcacgag cttctccaac tcgcggtgtg cggcgatgag cagcagcgct gcaccggctt
     5581 cgaaaccgat ccgccccttt atgcccaggg cggtttcccc cacatgaatg ccccggccga
     5641 aaccgtaggt ctcggcgagg tcgttcagct gctcgatcag tgtggctcca tcgagctgct
     5701 cgccatccag ggcgacgggc agcccgcacc tccaagcgag cacgacttcc cgcggtgatg
     5761 gcgcttcggc aggagggtcg agcagttcgg ccggcggtcc ggcccacgtg tcatgggtcc
     5821 atccgccgcc ccaggtcgtt ccccataggc cgcgattgac cgaataggca gttgcctggg
     5881 gaggaaccgg gagccttcgc tcggtcaaat aggcaacggc ctgctcgcgg gtgatgccga
     5941 gatcccggat gggagtcacg atctcgagat cgggcgcgag cacccggagc gcgacgtcga
     6001 aacggacctg gtcgttcccc gcgccggtgg atccgtgggc aaccgccgcg gcgccgattg
     6061 cgcgagccgt gtcgatgact gaaagcgctt gctgggtccg ttccgccgcg acgctgaggg
     6121 gatagacctc gccccggagc acatttccct gaatgagaaa gcgcacgaac cggtcgaaga
     6181 cggctgttcg tgcgtcgatc tcgtggtgcg ttagggcccc cactgcccgg gcctgcgccg
     6241 cgatggcggc gcgggcttgg ccggtcgttc cgccagtatt gacgtagacg gtgtgcaccg
     6301 accagccgtc cgcggcgagg cgtggcacgc agaagctggt gtcgaggccg ccggagaagg
     6361 cgagcgcgat ggttccgtta cgcataatgc atgattatgc agatggccga cctgcaatgc
     6421 aaggcgggcg agctgcggaa acgctggcgc ggtcagcggc tccgggtgta ctccagcgtg
     6481 cctgcgcggc gaagggcggg atagggcgcc gaccccttga gcgcgcgcca caaaatgcgg
     6541 ttgaactcgt cgtcctccac ccggtccacg gcggaaaaat ccagccgggc cgaccgccgc
     6601 gcccccgctc cgcgcgacgg attgatctcg gtgagcgact gggtaggggt gagcgccgcg
     6661 tagggccgaa ggtccgggct tcccgcccag atttcccgca acggcctccc gaagcggtcg
     6721 aactgggaga gcggaccgag gtccaggatc tcttcgatgg tggcgagtac gtcggtggta
     6781 ttgacgaagc ggcggaccag gcccgctcgg ctgtatgggg agatcaccag cagcacggag
     6841 cgatgggaat ccacgtgatc gggcccgtct tgcgcatcgt cctcgaggac gaacatcacg
     6901 gtgctcgccc agaacggtga atgcgaaagc gcgtcgacga tccggccgag ggccaggtcg
     6961 ttgtccgcca tgtaggcctt tggcgtcgga tagccggcgc gggcgcccgc agtgtgatcg
     7021 ctggggaggt ggatgatctc aagggtgggc atccgaccgg cggagacatc ggcctggaac
     7081 tccgcgaccc agacgtccgc cctccgctgg tcgggtacgc cgaggtcgaa cgagggatac
     7141 tgatcgctgg tatacggccg tagggcggcc cgggtggaac gatatcgagc cggaccgggg
     7201 tcgtccggct gcggcggaat gggctctccg tattcgccgt aaattctgag cgcgagcccc
     7261 ttcttgatcg cctggttcca gagaaatccc atcgcaggat cgtccacctc gccttcaccc
     7321 ggagtctccg ggcggcgcaa ccgatatatg tccggcgtgg tcttttcgac gtagtcggtg
     7381 acgtatccgg cggtagacca gggatggccc tgcgcgctca cttcagcgtt cacaaagaac
     7441 cgatcgtaaa tcccgaaccg ctccgccagc gcgtgatggt tcggcgacat gttgcgagga
     7501 aagaacagga gcgacgtgtc gccatcggcg cgcggcaggt cactcagcac ctggtcgaag
     7561 gtgcggttct ccttgatcac gtagatgacg tggtgaaacg gcggatagtg agcctgccga
     7621 ctgggcgacg catcccaacc gttcgcgtgc gccacgcggg cagacaatga atcgagagcc
     7681 cggcccgcgg ccttcgcagg cagaatcgtc accgtcccgt tgagctggcc cagggtgtac
     7741 aacgagtcgc ctttcgccag cggggtgtcc ggttgcgccc cacgcggatt aggaccggct
     7801 cccccaccct tgccgttcac cgcccaaaga gagtcgtcca ggaggaccaa cgcagtggga
     7861 taccactcgg tcggaattcg cccggccagg cgatcgttcc cgtgtgccga gctgatgccc
     7921 gatacgccag cgcttagatc gaaaacggcg acagcattgg catcagcctc ggcgaccaac
     7981 agtcgggtac cgtcgagaga caacgtcagt gcgttcgggg tactgccctc gagcacgccg
     8041 gacggaggcg ggtcggtcag gcgcgcgatg actctcccgg cccgtgtgtc gaccacagcg
     8101 atctgatccg tgctggccga cgtcgtgaac agcctgctcc cgtccgcgct gaggcagagc
     8161 gccgatgggt gacgccccac ctcgatgctg ccgtcggccc ggagcccatc cggggtagcg
     8221 cgaaagacgc cgatggtcga accaccccag gccgacacgt agacccgatc gtccggggcc
     8281 acagcgacat cgtatggata gctcgacatg ccgagtcgct ggcgcacgcg gtgagtcgcg
     8341 aggtcgagga tcgcaatcga atcggccagg ttctccgcca cgtagagaac gcgaccgtca
     8401 cgggaaaggg ccaggccggc agggtatcgg tggactgtgg tgtcggatga gtcggccctc
     8461 aaggtgatgc tgtccgacag gaaggccggc tctggtcggg cggggtccca agtgtagagg
     8521 tataccattt ccgccgaacc gcctgaagcg tagagaatcg agccgtccgc ggcccaggca
     8581 agaccaagga acgcccccgg ctggggaagc cgttgcacga ccacacctcg ggtgcgatcg
     8641 agtatttcga ttccctgttc ccgccacccg ctcaggctga gcaccagata gcgtccgtcg
     8701 ggggagactc gcgcagccag cggcatgttt ccgactgcca gtgaacggcc cgccggatcg
     8761 agtcgcgcac ctgtgggaag gcgatgactt gccgcgggtt ccgagggaag tccgacctcc
     8821 tcggcttgag ggcgctggca gcttgcgagc gcgagcagca aggcaaacgg cgcacctgtc
     8881 agactcggca tccgtcgaga acgattcacc tgagctggct ccgtccagag cggttttctt
     8941 gacgtgcgtc aatcgagtgc ttcggagcac gcgggccaaa ttgaatcgca ttgccattcg
     9001 tccactagtg gacgaagaag cgccccgctt gcgtcgtagc ttgcatgatg atacagttgt
     9061 atcataaggt cagcgatagc gtatcatgtc accatcccgg ggtatcggag caatttggac
     9121 caggctgcgc cgaagcgtcc tgcggccatc cgtgccgaca gcgttctcgc ccggttccca
     9181 gtcaggctcc ggcctcctgc ccggctacgt tgcgctcggc tgctggcaac gcgggggagc
     9241 agtcctggac gaaaggctcg agctgctcat cggacagttg tcggccgagc tcagcgggtg
     9301 ccgttggtgc atcgagcagg gacggcaccg ctggcgcaaa gcgttgctgc ccgttgttct
     9361 actgcagcaa ctgcgcgacc agtacacgag cgctttgttc tccgaacgcg accgtgcggc
     9421 actgatgctc accgaggccg tggcccgcta caccgggcga gtggctgccg ccgcagacca
     9481 agccgtcgct cgggcacggt gtcatttcac ggaatccgag attgcccgga tcgctcatct
     9541 tgccgcagtc gagcatttct acgatccggc cacgggagca gtcggccagg acgtcgatga
     9601 gctgaactgc aaagccgtca tgccgtctcc ggctattgcg ccagggttcc ggacccgcgg
     9661 ccagccatga gccccgctcc gactcctggt cggcgctggc ttctgctgat tcatcagctc
     9721 ccgcccaagc ccgattatct gcgagtcaag atctggcgac gcctacagcg catcggcgcc
     9781 gtcgccctca agaactccgt gtacgtgctt ccccgtaccg accagaccgt cgagcacttt
     9841 caatggatcc tgcgggaaat cgagtccagc ggcggcgagg cgtcggtgtg cgatgccgcg
     9901 ttcgttaccg gcctctcgga cggccagatc gaaacactgt tccgcgcggc tcgtgaggcg
     9961 gactatgccg cgctttcgga ggaggtgcgg gcgctatcgc gaggtacgat cgcgcggcgc
    10021 ggcgctgaca tgggccgaac cgacgtcgag gcaacgctaa ctcgcctccg gcgccggctt
    10081 caggagatcg gagccattga tttcttcggc gcgccaggtc ggcagattgc ggaggccgcc
    10141 atcgagcggc tcgaatcgca gattcgctcg accggacgca aatccggcgc ctcgatcggg
    10201 aaaaccaaag gcaaggcaag gaccggtcga atctgggtga cccgccgagg ggtccacgtc
    10261 gatcgcatcg ccagcgcatg gctgatctcg cgatttgtcg acccgaaggc ccggtttcga
    10321 ttcgtggaag agcaaccgta tcagcccaag ggcgatgaag ttcgcttcga catgttcgag
    10381 ggcgagtaca ctcacgaggg cgatcgctgt acgttcgaaa cgttgattgc ccgcttcgag
    10441 ctgacgcaac caggcctgca ggcactcgcc gagatggtgc atgacatcga cctcgccgat
    10501 ggcaaattcg ggcgggcgga gacggcagga ctcgagcgtc tcatcgacgg gatcacccgt
    10561 gcgcacgtcg aggatgacga gcggatcgcc cacagcgcga tcctgttcga cgattttctg
    10621 agatcatttc agcacgagac tggcccgaag aaacggtcgg cgaggtcacc atagtttcca
    10681 ttgccactgt actggtaaac cgccttcgcg aggctcgccg ggttacggtg ctcaccggtg
    10741 ccgggatctc ggcggaaagc ggcgtggcaa cttttcggga cgcgctgacc ggcttgtggg
    10801 cccagtatcg agcggaggac ctggcgacgc cggaagcctt cgccagaaat ccggcgctgg
    10861 tgtgggattg gtatcggatg cgccggcaaa tggtggcacg agccgagccg aacgccgggc
    10921 accttgcgct ggtcgagatg gaggcccaga tcccggagtt cgtcctcatc acgcagaacg
    10981 tcgatgggct tcaccaacgt gcgggcagca gtcgtccgat cgagctgcac ggcaatatca
    11041 cccgggcgcg atgctcgcgc gaggacacgc cggttgacca ctgggacgag acggccgatt
    11101 caccgcctcg ctgtcttgcg tgcggagcgt tcttgcgtcc ggacgtggtt tggttcggcg
    11161 agctgcttcc gctcgaagcg ctccgcgccg ccgagatggc ggcgcgccac tgtgacgtct
    11221 ttctatcggt cgggacctcg aacctggtag agccggccgc gtcgctgccc tggacggcgg
    11281 ccgcggccgg ggcagaggtg gccgtgctca acgtttccat ggatggccag cgtcgcggac
    11341 ctgccatcga gcatctggtc ggcccggctg gtgagctact cccggccctg gttcaggcca
    11401 cctggccgaa gcggtgagga cctaccagcg ttccgcgatc gcctgaacca tgtcccccaa
    11461 cccgatcaca tcacgaagat cgattacctc ggcaggcgag tgactgtagc gcagggccca
    11521 accgatggcc acatccggca caccccacgc ggcgaaggcg gcaccgtcgt tgccaccatt
    11581 cgtggtaccg atttgaagct ggatgccgtt cacccgggcc agacgcgcca acgagtcgac
    11641 cagtgccggc ggagcaaccg agttgttatc gagcgcccga gcgacgggcc cacgcccaat
    11701 tggagcgacg gcgaatgtct gcggctctag cggcgaatcg gccgatacga aggtgtcaat
    11761 cgcatgaacc cgcaccggcg ttcccccgag ggacgcggca gccgcggctg ccccttccaa
    11821 cccgatttct tcccgtacgc tgaaaatgaa ggcgacttcg tgccggaggc gttccgggtt
    11881 cagccggcgc agcgcgagaa tcaatgcggc gcacccgacc cgatcgtcga atgagcgtcc
    11941 ggtggcacgg gtgcctgcca gccgcacgta gtgcttgggc atcgtgatgg tatggccaac
    12001 acggacaccc agcgactcgg tagctgcccg agtgtcggtg cccacgtcca cacgaaaacc
    12061 ggccggcgcg cgctcaggct tcgatccgac cgagtcacgc ggcatgaaga cgccgggcac
    12121 tataccggtt ccggtatgga tcaacgcagg ctgggcctcg aacagcgaag ggaagaagcc
    12181 accccgtgtc tcgatctcga gcgatccatc ctccctgatt gccttgatct cgaacccgat
    12241 ctcgtccagg tgcgcgacga atacgatcaa tggatcgcct ttacccacgc gcacccacaa
    12301 gttgccggcc gtatcggttt cggccttttt tttggcccac tggggaagca gccgctccac
    12361 cgtcgcccgc acgggcgcct ccgcacccga tacgccatag ctctcgacca gtggggccag
    12421 cacggcctgg gcctggccaa cgcgatcgga ttgctgagcg gtcagccgcc cggcaaagcc
    12481 cgcgacgagc ccgagaagaa gcagcaccct catcgtgcac cctccccctt gagccagctg
    12541 gacatcgtcg cgttgagccg atcgacttca ggcaacgaga cggtttcaac cggggtgccg
    12601 ggatagcggg t
//
LOCUS       DASRJQ010000362         4389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_369744, whole genome shotgun sequence.
ACCESSION   DASRJQ010000362 DASRJQ010000000
VERSION     DASRJQ010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369744"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2085
                     /locus_tag="VFB89_08065"
     CDS             <1..2085
                     /locus_tag="VFB89_08065"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM27298.1"
                     /translation="LREIRIAARLVHSQIVPVHDSGECGAFLYFVMPYLDGESLRQRI
                     DREERLPLADAVRIGRSVAGALDYAHRSGVLHRDIKPENILLHEGEPVVADFGVARAV
                     SAAFGQGRTVSEPGIAIGTPAYMSPEQASADEDLDGRTDIYSLACVVFEMLTGRPPFA
                     GTTPRQTMARHVVELPPALRTLRPDAPPLLEQALARALAKNPAERFATAGEFGRALEA
                     TSPLATAPLAAVAPARADRHAIAVLPFVNASPDPDTEYFADGMTDELIDALTKVEGLH
                     VASRTSAFAFKGKQTDVRTLGALLGVAVVLEGTVRKAGPRLRITARLTDVRDGRHLWS
                     QRYDRDLHDVFAMQDEIAQTIVTTLRATLLGELGDVLPRRYTANLTAYNLYLKGRYSW
                     NKRTAEGVADGVRYFEEAIARDQGYALAYTGLADCFALQLDYRGVPVAEGMQRAKEFA
                     LKAIALDDTLAEAHTSLGWVQFIYEWDWESAAQAFRRALELNPRYATARQWHSWLLIA
                     QGRVEEALAEGRLAFELDPASVSIRRSLGWLMYYARQYDGAAEHLRRAVELNPTSEET
                     HRIFGLVRLQQGRLDEAENEFREAIGLSTELAYARAGLAHVAACMGRPDEARSILAGL
                     YAQAKERYVSPVAFATVHLGLEEADHAFAWIERAREERRGWLCYLKVEPLLDPIRSDP
                     RFAGLLEKMRLT"
     gene            2233..2640
                     /locus_tag="VFB89_08070"
     CDS             2233..2640
                     /locus_tag="VFB89_08070"
                     /inference="COORDINATES: protein motif:HMM:NF023514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HZM27299.1"
                     /translation="MEGGKTKWARLALTAALLAPAGGCVAAAAGAAGAGSAIYLTSRG
                     ASSTVNGSVNDVAARTRSVLDGEGITITETKTESEGDERSFKGTKGDLDVSVDLKRET
                     PTTTKTEVSARKNLAEWDKDYAKTLLNKIVKGG"
     gene            complement(2647..3693)
                     /locus_tag="VFB89_08075"
     CDS             complement(2647..3693)
                     /locus_tag="VFB89_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27300.1"
                     /translation="MITTRWAVAPRWLARAVLFGLVLLIGYGARQSWRTPLRPRSPSA
                     AGTNTDVDVYRRIADRVRHGEPYYMVARAELAAAGFPTSSVFNFRLPTLAWLLGVLPS
                     DRTGQAVLIGLAALTLILWFATLWQRESFAIGLAGSALLLGLLAWPALDDAYLVHDLW
                     AGVLIALSLVALGRGWFAASIAAGAAALFVRELSLLYVCLGAADALAHKRRRHAVAWA
                     LVIVAFGLVLIVHAKAVAISAVSTVGATRATSWLRFGGWEFVLKTALMNAWLIGMPPV
                     LVAAILPIALLGLTGWRGSPGTQVATTVFAYVAAFLVVGQPFNHLWGFLYVGPALMGL
                     AFAPAALRDLWRRA"
     gene            complement(3690..>4389)
                     /locus_tag="VFB89_08080"
     CDS             complement(3690..>4389)
                     /locus_tag="VFB89_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27301.1"
                     /translation="EQLRLRGVPVRSTPAGSAWSVVPPSLYALAATTLVAGLAASTAL
                     PTLLASSPLVAGLLVLGAHRAVQRPVLTPRTRRSSLPPALGQRIAETLGGLPLGTARS
                     LLADLARVSESVFSGPRSAAVASELSELLEHACDAARDLAALDDTLGLLEKRRETATA
                     VAHWAEGHSGVERARDRLVQQLLEALTVVSRLHTSAVSSDAAGGELAGVAHELAVRSE
                     AHAAAMREVEALLA"
BASE COUNT          797 a         1460 c         1496 g          636 t
ORIGIN      
        1 ttgcgcgaaa tacgaatcgc ggcccggctg gtacactcgc agatcgtgcc ggtacacgat
       61 tccggcgagt gcggcgcttt tctctacttc gtgatgccgt acctcgacgg cgaatcgctg
      121 cgtcagcgga tcgaccggga agagcgcctc ccgctggccg acgcggtccg catcgggcgc
      181 agcgtcgccg gcgcgctcga ctacgcccat cggagcggcg tgctccatcg cgacatcaag
      241 ccggagaaca tcctgctgca cgaaggcgag ccggtcgtgg ccgacttcgg cgtggcccgc
      301 gcggtctcgg cggccttcgg ccagggccgc accgtgtccg agcccggcat tgccatcgga
      361 actcccgcct acatgagtcc ggagcaggca agcgcagacg aggacctgga cgggcgcacc
      421 gatatctaca gtctcgcctg tgtcgtgttc gagatgctga cgggccggcc gccgtttgca
      481 gggaccacgc cacgccagac gatggcgcgg cacgtggtgg agctgccgcc ggcgctccgt
      541 accctccgtc ccgatgctcc gccgctgctg gagcaggcgc tcgcgcgggc cctggcaaag
      601 aatccggcgg agcgcttcgc cactgcgggg gagttcgggc gcgcgctcga agcaacatct
      661 ccgcttgcaa ccgcgccgct cgcagcggtc gcgcctgcgc gggccgaccg gcacgccatc
      721 gcggtcctgc cgttcgtgaa cgcgagtccc gatcccgaca ccgaatattt cgccgacggg
      781 atgaccgatg agctgatcga cgcgctcacc aaggtcgagg ggctgcacgt cgcctcccgc
      841 acgtcggcct tcgcgttcaa gggaaagcag accgatgtcc gcaccctcgg cgcgttgctc
      901 ggcgtggccg tcgtgctcga agggaccgtg cggaaggcgg ggccgcggct ccgcatcacc
      961 gctcggctta ccgacgtacg ggacggtcgg cacctctggt cccagcgata tgaccgcgac
     1021 ctgcatgacg tgttcgcgat gcaggacgag atcgcgcaga ccatcgtgac cacgctgcga
     1081 gcaacgctgc tgggtgagct gggcgatgta ctgccgcggc ggtacaccgc gaacctgacc
     1141 gcgtacaacc tgtacctcaa ggggcgctac agctggaaca agcgaacggc ggaaggtgtg
     1201 gccgacggtg tgcgctactt cgaggaggcg atcgcacggg atcagggata cgcactcgcc
     1261 tacacggggc tggccgactg ttttgccctc caactcgact accggggtgt tccggtggcg
     1321 gagggaatgc agcgggcaaa agagttcgcc ctcaaagcaa tcgcgctgga cgacacgctg
     1381 gcggaagccc atacctctct cggctgggta cagttcatct acgagtggga ctgggagagt
     1441 gcggcgcagg cattccggcg ggcgctcgag ctgaatcccc gctacgcaac ggcgcggcag
     1501 tggcattcct ggctcctgat tgcccagggc agagtcgagg aagccctggc tgaagggcgg
     1561 cttgccttcg agctggatcc cgcgtccgtg tcgatccgcc gcagcctggg ctggttgatg
     1621 tattacgcca ggcagtacga cggcgcggcc gagcacctgc gacgggcggt ggagctgaat
     1681 cccacgtcgg aggaaactca tcggatcttc ggactggtcc ggctgcagca gggcaggctg
     1741 gacgaggcgg agaacgagtt tcgcgaagcg atcggattgt cgaccgagct cgcctacgcc
     1801 cgcgccggac tggcgcacgt tgcggcatgc atgggccgtc cggacgaggc acgttcgatt
     1861 ctcgccggac tctatgccca ggccaaggag cgttacgtct cgccggtagc atttgccacc
     1921 gtccacctcg gactggagga ggctgatcac gcgttcgcct ggatcgagcg cgcccgggag
     1981 gagcggcgcg gatggctgtg ctatctcaag gtcgagccgc tgctcgaccc gatcaggtcg
     2041 gatccccgct ttgccggtct tctggagaag atgcgcctca cctgaggggt gggtcgcgcg
     2101 tcaccgattt tccctatact ccagaccaag catcatctat gtcgcaggtt cgctggaccc
     2161 tgcgcggcgc aaatgctatt tcccatacac ctcacacgct ggtagtctga gagttttttc
     2221 ttggaggaag ccatggaagg cggtaaaacg aaatgggccc ggctggcgct cacggcagcg
     2281 ctgctcgcgc cggcaggcgg ctgcgtagcg gcggcggcgg gagcagccgg tgccggatca
     2341 gcgatctacc tcacgagtcg cggcgccagc tcgacggtta acggctcggt caatgacgtc
     2401 gcggctcgaa cgcgctcggt gctcgacggc gaaggaatca ccattacgga gaccaagacc
     2461 gagtccgagg gtgacgagcg gtcgttcaag gggacgaagg gcgatctcga cgtctcggtc
     2521 gatctcaagc gcgagactcc caccaccacc aagaccgagg tgagtgcacg gaagaacctc
     2581 gctgagtggg ataaggatta cgccaagaca ctgctcaaca agatcgtcaa gggcgggtga
     2641 gcgctactac gcccgccgcc atagatcccg cagggcggcg ggtgcaaagg ccagacccat
     2701 cagggcgggc cccacgtaga gaaagcccca gagatgattg aagggctgtc cgacaaccag
     2761 gaacgccgca acgtacgcaa agactgttgt ggcgacctgg gtgccgggtg agccgcgcca
     2821 acccgtgagg ccgagcaacg caatcggaag gatggccgca acgaggacgg gcggcatgcc
     2881 gatgagccag gcgttcatca aagcggtctt gagcacaaat tcccagcccc cgaaacggag
     2941 ccagctggtt gcgcgggtag ctccgacagt gctcacggcg gaaatggcga ctgctttggc
     3001 gtgcacgatt agcaccaggc cgaacgccac gatcaccagg gcccaggcca ccgcgtgccg
     3061 gcgccgctta tgcgccaggg catccgctgc gccgagacag acgtagagca ggctcagctc
     3121 tctcacgaac agagccgccg cgcccgcggc gatcgaggca gcgaaccagc cgcggcccaa
     3181 ggccaccaaa ctcagtgcaa tcagtacgcc cgcccagaga tcgtgaacca ggtaggcgtc
     3241 gtcgagtgcg ggccaggcaa gcaagccaag cagcagcgcg ctgccggcca gaccgatcgc
     3301 gaacgactcc cgctgccaca gcgtcgcgaa ccagagtatc agcgtcaggg ccgccaggcc
     3361 gatcagcacc gcttgcccgg tgcgatcgct cggcagtacg ccgagcagcc atgcgagcgt
     3421 gggcagtcga aagttgaaga cgctgctggt gggaaagccg gccgcggcaa gctctgcccg
     3481 ggcaaccatg taatacggtt cgccgtgtcg aactcggtcc gcgatgcggc ggtacacgtc
     3541 gacgtcggta ttggtgccgg cagcggaagg gctgcgaggt cgaagggggg ttcgccacga
     3601 ctgccgggcc ccatacccga tcagcagcac gagcccgaaa agcaccgccc gggccagcca
     3661 gcgaggagcc acggcccagc gcgtcgtgat cacgccaaca gtgcctcgac ctcccgcatt
     3721 gccgccgcgt gcgcctcact gcgcaccgcc aactcgtggg cgacccccgc caattcgccg
     3781 ccggctgcgt ccgacgagac ggcgctcgtg tgcaagcgac tgacgaccgt gagcgcctcg
     3841 agcagctgct gcaccagccg gtcgcgggct ctctcgaccc ccgaatgacc ctcggcccag
     3901 tgtgcgaccg cggtcgcagt ctcgcgccgc ttctcgagga ggcccagtgt gtcgtccagt
     3961 gccgcgagat ctcgggccgc gtcgcacgcg tgctccagga gctcgctcaa ctccgaggcc
     4021 accgcagccg agcgaggtcc ggagaagact gactcgctga cccgggccaa gtcggcaagc
     4081 agactgcgcg ccgtacccag gggaagcccg ccgagagtct cggcaatgcg ctgaccgagc
     4141 gcgggcggca ggctcgaccg gcgcgtccga ggcgtcagca ctggccgctg aaccgcgcga
     4201 tgtgccccga ggaccagaag acccgctacc aggggtgagc tggcgagcag cgtagggagg
     4261 gcggtactcg cggccagccc ggcgaccagc gtcgtggcag ccagcgcata cagcgacggg
     4321 ggcacgacgc tccacgcact gccggccgga gtgctccgaa ccgggacgcc gcggagccgg
     4381 agctgctcc
//
LOCUS       DASRJQ010000363         6566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2835987, whole genome shotgun sequence.
ACCESSION   DASRJQ010000363 DASRJQ010000000
VERSION     DASRJQ010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6566
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2835987"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..122
                     /locus_tag="VFB89_08085"
     tRNA            50..122
                     /locus_tag="VFB89_08085"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:83..85,aa:Ala,seq:tgc)
     gene            481..957
                     /locus_tag="VFB89_08090"
     CDS             481..957
                     /locus_tag="VFB89_08090"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HZM27302.1"
                     /translation="MTRCTAVGLLALSLALPPRAFAQAGAGDLRGQIAKIGQAWEKAY
                     NAGDAAAVTALYVTDARLMVPGAEPGSGPKSIQELIAKDIALGGKLTLTTEDVVGFGD
                     YALETGKWVATSADGKHLDHGPYVTLYKKANGGWKIYRDIWNSSMTSQAGVAPKKP"
     gene            complement(1167..1967)
                     /locus_tag="VFB89_08095"
     CDS             complement(1167..1967)
                     /locus_tag="VFB89_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27303.1"
                     /translation="MITLKIMASVVATFALTLVVGCNRTDLTAPGAQEAVALQTKQVS
                     ATKKLSATLCSPSGGGFTLASTNPYWPLTVGRQLILTGEEEGATHTLQITVLDQTRPI
                     SGVTTRVVEEREFADGELIEVSFNYFVQAPGGAICYFGEDVDIYEDGGVSHEGTWCAD
                     HPGFAAGIFIPAEPTPGTRFEIEVAPGVAEDEGKIVGVGSVEVPFGLFTNTIRIREFN
                     PLDRGKDYKVHAAGTGIIVDGTLQLEDVNQTSGAAPQPTITRQTCGVS"
     gene            complement(1987..2328)
                     /locus_tag="VFB89_08100"
     CDS             complement(1987..2328)
                     /locus_tag="VFB89_08100"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HZM27304.1"
                     /translation="MKLRLPPPLLLGMMVVLLACHSSAGPGQSIRFDPPNTEFATRLG
                     ITPPPPITQEQAMAIAAQAAGGTALSATQESEAGHVLFEVKVQTSSGRKEVEVRASDG
                     GVVGIEPDDND"
     gene            2644..4857
                     /locus_tag="VFB89_08105"
     CDS             2644..4857
                     /locus_tag="VFB89_08105"
                     /inference="COORDINATES: protein motif:HMM:NF011071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming)/trehalose-phosphatase"
                     /protein_id="HZM27305.1"
                     /translation="MPRLILASMRLPVTVRQTPDGPRVTESVGGVATGLRALQVKGSG
                     PWVGWPGAADDGHIDRGVLDRQLKGFGCVPVHLTLPEVRGFYVGYANGVLWPLCHYLI
                     DQLPVRSRHWNTYESVNARFADTLAELYRPGDLVWIHDYQLMRVPALLRERIPGARIG
                     FFLHIPFPSFEVFRVLPTRERILEGLLGADLVGFHTAAYARHFAVSAGELLDLVVDGT
                     PEEAIVHHAGGETGVGVFPMGIDADRFGGLAEKADPAAHRRSLGVTRRERLLVGIDRL
                     DYTKGIPRRLLAFEHLLRTHPELREHVTLLQVAVPSRTGVSAYRRLRRQVDAMVGRIN
                     GLFGTPAWTPVRYLYHGFAQAELVGLYRTADVMLVTPVRDGMNLVAKEFVASRPDEDG
                     VLLLSEFTGAASELAEAMIVNPYDIDETTAALFRALTMSRDERRSRMQALRRRVTQHT
                     LERWRRAFVSALEGRGRSSPQEEPRARPSPTTQVRAVLDELRAAPALVLFLDYDGTLV
                     PFAPAPELARPDAALLDLLGQLATRPRTDVHLVSGRQQSVLEQWFGRLPIGLHAEHGA
                     WSRLRGERLWRRHPAVRPMPYDDLLALLKRYTRETPGALIERKAAGLSWHFRMARPDQ
                     GARHAERLFEEAEERWPGDTVEVLRGSMVLEFRPAGVHKGLIIPQALAAAPPDARAVA
                     IGDDRTDEDLFESLPEGSVGIHVGAGPSAAGLRLRDPEACRAFLAGLLAPVPATV"
     gene            4969..5241
                     /locus_tag="VFB89_08110"
     CDS             4969..5241
                     /locus_tag="VFB89_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27306.1"
                     /translation="MKRWRSLVPKRESGQAVLEYALVLALVAAGLLVGLLLFRSSLGN
                     AYGRVAHRVQSRSSPASESGAIADTVGGEAGVARPGAEAAEADKER"
     gene            5246..5599
                     /locus_tag="VFB89_08115"
     CDS             5246..5599
                     /locus_tag="VFB89_08115"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZM27307.1"
                     /translation="MSSSRPRLHVRLHYEGATRPSLILEAGSLNVLDLSERGLRYRQE
                     FGPEPALGAKLEGILRLTSGETLPVRGTVVRVMRPAKPEDASVIEVAAHLTDVGIPSR
                     VILSERKALQEPPAH"
     gene            5674..6447
                     /locus_tag="VFB89_08120"
     CDS             5674..6447
                     /locus_tag="VFB89_08120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZM27308.1"
                     /translation="MVVPCYNEAARLDVSRFREFAAASADVGLLFVDDGSTDTTSAVL
                     TELVHEVPNRIRGIRLAANQGKAGAVRAGVLVALASDPTFVGFLDADLATPLSAVNRF
                     VAALEARPELVGVIGARVKLLGTVIDRSAARHYLGRVFATAASLVLKLPVYDTQCGAK
                     LFRAGPLVRAAFGAPFRSRWAFDVELLARLGQAGSGAGPRPAGNLVIELALEEWRDVK
                     GSKLAPHAMLRAGLDLAFVRRNARRDAGRSLPPPAFTWL"
BASE COUNT         1051 a         2123 c         2149 g         1243 t
ORIGIN      
        1 ggcgctgcgt tggcggggcg cgggcgatcc cactattctg ttggagccag gggcggtagc
       61 tcagctggga gagcacctgc tttgcaagca gggggtcgcc ggttcgatcc cggtccgctc
      121 cactcgccgt aagtccctcc aactaacccg gcccaaagag ccctcggctg ctgcagttcc
      181 cgcctcggcg cgcccactct aaccaatgcc cggcggacga gactcactgc ggtatgaaga
      241 cttcttggaa gctccggcat gtaactggag gtgcgcgttt gcagccgcca gctccgccgc
      301 agggcgacct agcggaaggg cgggcccgcc gccatcctag ttgcacgtct ctgccggggg
      361 acttggatgc gctgccgtac gcggtttctg ctatccgcac tccgcatgtt tgcactctgc
      421 cgaccaagtt cgacctggac cccaatcctc cgcacgatca cccatctgac tggaggcctt
      481 atgactcgct gtaccgcagt aggcctgctc gcgctgtccc tggcgttacc gccccgggcg
      541 ttcgcgcagg ccggagcagg agaccttcgc ggccagattg ccaagatcgg ccaggcatgg
      601 gaaaaagcct ataacgctgg agacgcggct gccgtcacgg ccctatacgt gacggacgcc
      661 aggctcatgg ttcccggggc cgagccgggc tccggcccga agtccattca ggagctgatt
      721 gcaaaggata ttgccctggg cggcaagctc accttaacca cggaagatgt cgtgggcttc
      781 ggtgactacg cgctcgagac aggcaaatgg gtggcgacct ccgccgacgg caagcatctg
      841 gaccacggcc catatgtcac gctgtacaag aaagcgaacg gtgggtggaa gatctatcgg
      901 gacatttgga acagcagcat gacgagccaa gcaggtgtcg cccccaaaaa accatgagcc
      961 gggttggcta ttgagaatcg gtgccgctgg gcgcgagcag acaccaacgg ctcgggtgga
     1021 ttagtccccg agccgttcag tgctccagcg ccggcaccga cgtacacgag cccgcggaac
     1081 catcgttatt tcggttcaag cgtcttggcc ggatccccgg ttcgctgcat cgacgctcgc
     1141 ttgcggccga cgctccggag agcgacctat gacaccccgc aggtctgccg agtgatcgtg
     1201 ggttgcgggg ccgcccccga ggtttgattc acatcctcca gctgaagcgt tccgtcaacg
     1261 atgatcccgg tcccggccgc gtggaccttg tagtccttgc cccggtccaa aggattgaac
     1321 tctcgtatcc ggattgtgtt ggtgaagaga ccgaaaggca cttcgacgga accgacgccc
     1381 acgatcttgc cctcgtcctc cgccacgccc ggtgcgactt cgatctcgaa tctggtcccc
     1441 ggggtcggct cggcggggat gaagatcccg gcggcaaagc cggggtgatc ggcacaccag
     1501 gtaccttcgt gggaaacgcc tccgtcctca tagatgtcta catcctctcc gaagtagcag
     1561 atggctccgc ccggtgcctg gacaaagtag ttgaaggaga cctcgatcaa ttcgccgtcc
     1621 gcgaactctc gctcctcgac cacccgggtg gtaacccccg agattggccg agtctgatcg
     1681 agtacggtga tctggagagt gtgggtcgca ccctcctcct cgcccgtgag aattagctgc
     1741 ctgccgacag tgagtggcca gtatggattt gttgacgcca gtgtgaaacc accaccgctg
     1801 ggggagcaga gcgtggcgct gagctttttc gtggcgctca cctgcttggt ctgaagagcc
     1861 accgcttctt gcgcccccgg cgcagtcagg tccgtccgat tacagccgac taccagcgtg
     1921 agcgcaaacg ttgctacaac gctggccatg atcttcagcg ttatcatggg ttcctctcga
     1981 ggactctcag tcattgtcgt ccggctcaat tcccacaaca ccgccgtccg aggcccgcac
     2041 ttccacttcc ttccgtccgg aggaggtctg gaccttgact tcgaacagaa cgtggcccgc
     2101 ctcgctctct tgggttgcgc taagagcggt gcctccggcc gcttgtgccg cgatcgccat
     2161 ggcttgctcc tgcgtgatag gaggaggagg ggtgatgccc agccgggtgg cgaactccgt
     2221 atttggggga tcgaaccgga tcgattgacc ggggccggcg ctgctgtggc aggcgagcag
     2281 cacgaccatc attccgagca agagtggggg agggagccgg agtttcatga tctgtctcct
     2341 ggaggcggag agagtggcag cacattccag taccgaggag gtactgcgcg cctcgtcatt
     2401 gcgccatcgg tggtaccgat aggggaacag acgaagcgga ggtttgtgac agccgtgatc
     2461 tccaggcttc tctgaccgtt ccggacatgg ttgcggaccc ttcagacgac ggatagcctt
     2521 tctgggacgg aggcgccgga tcggtccggc cgcatctccc actcgttcac gacccgctgc
     2581 tccgttgcgc ccggcgcgtt tcggcacgcc ggctgttcgc gacgtgcggc gcagagagca
     2641 ggcatgccac gactcattct ggcctcgatg cggttgccgg tcaccgtccg gcaaacgccg
     2701 gacgggcctc gagtcactga gagcgtaggt ggagtagcga ccggattgcg tgccctgcag
     2761 gtgaagggta gcggtccgtg ggtgggttgg cccggcgctg ccgatgacgg tcacattgac
     2821 cgcggcgtgc tggaccgaca gctcaaggga ttcggctgcg tcccagtcca cctcacgctg
     2881 cccgaggttc ggggcttcta cgtcggttac gccaatggcg tgttgtggcc gctgtgtcac
     2941 tatctgatcg accagcttcc ggttcgttcg cgccactgga atacctacga gtctgtcaac
     3001 gcgcgcttcg cggacacctt ggcggagctc taccggcctg gtgatctcgt ctggattcac
     3061 gactaccagt tgatgcgggt tcccgcgctg cttcgggagc gaatccccgg cgcgcggatc
     3121 ggcttcttcc tccacatccc gttcccatcc ttcgaagtat tccgcgtgct gcctacccgg
     3181 gagcgtatcc tcgaaggctt gctgggcgcg gacctggttg ggttccacac cgccgcctac
     3241 gctcgccatt tcgctgtctc agccggcgag ctgctcgatc tggtcgtcga cggcacgccg
     3301 gaagaagcga tcgttcacca tgcgggtggt gaaaccggtg tcggtgtgtt tccgatgggg
     3361 atcgatgccg atcgcttcgg cggcctggct gagaaggcgg accccgccgc ccatcggcgg
     3421 tcactgggcg taacccgccg cgagcggctg ctggtcggga tcgatcgtct cgactacacc
     3481 aagggcatcc cgcgccgcct gctcgcgttc gagcacctgc tacgcactca tccagagctg
     3541 cgagagcatg tgacgctgct tcaggtcgcg gttccttccc gcaccggcgt gtccgcctat
     3601 cgaaggcttc ggcgacaggt cgacgcgatg gtcggccgga tcaacggatt gttcggcact
     3661 ccggcgtgga cccccgtccg ctacctctat cacggattcg cgcaggccga gctggtcggt
     3721 ctttaccgga ccgccgatgt catgctggtg acgccggtgc gagacggaat gaatctggta
     3781 gccaaggagt tcgtcgcgtc gcgaccagac gaagatggcg tgctcctgct gagcgagttc
     3841 accggggccg cctccgaact ggcggaggcg atgatcgtga acccgtacga catcgacgag
     3901 accaccgctg ctctcttccg cgcgctcacc atgtcccgcg atgagcgtcg gagccgtatg
     3961 caggcgctcc ggcgcagagt cacccagcac accctggagc gttggcgccg ggcgttcgtc
     4021 agcgcgctcg agggccgagg acgcagttct ccacaggaag agccgcgggc ccggcccagc
     4081 ccaacaaccc aggtgcgcgc ggtgctcgac gagcttcgcg cggcgccggc cttggtactg
     4141 ttcctcgatt acgacggcac gctggtgcct ttcgcgccgg ccccagagct tgcccgcccg
     4201 gatgcggcct tgctcgacct gttgggccag ctggcaacgc gtccacgcac cgacgtccat
     4261 ctcgtgagcg gccgccagca aagcgtgctg gaacagtggt tcggtcgact ccccatcggg
     4321 ctgcacgcgg agcacggcgc atggtctcgc ttgcgcggcg aacgcctctg gcggcgccat
     4381 ccggccgtgc ggccgatgcc ctacgacgat ttgctcgcac tcctcaagcg gtacacccgg
     4441 gaaacgcccg gcgcgttgat tgaacggaaa gcagcgggtc tgtcctggca tttccgcatg
     4501 gctcgcccgg atcagggcgc tcggcacgcg gagcggctgt tcgaggaagc ggaggagcgc
     4561 tggcccggcg atacggtcga ggtgctgcgg ggaagcatgg tactcgagtt tcgtcccgcc
     4621 ggcgtccaca aggggctgat cattccccag gctctggctg ccgccccgcc ggacgcccgc
     4681 gcggttgcga ttggggacga ccggaccgac gaggaccttt tcgaatcgct gcccgagggc
     4741 tcggtcggca tccacgtcgg ggctggcccg agcgccgccg gtctgcgcct gcgcgatccc
     4801 gaggcgtgcc gggcgtttct cgccggcctc cttgcccccg ttcccgctac ggtctaatcc
     4861 tctcacccct tccccaagca ggcgatttcc gtagctttag ggctacatca ctccatatcc
     4921 gctgccccct ggacttcagg tcccaacccc cggtgtgccg gaggtgccgt gaagcggtgg
     4981 cgatcgctcg tgccgaagcg ggaatccggt caggcggtgc tcgagtacgc cctggtgctt
     5041 gccctggttg cggcgggctt gctcgttgga ctgttgctct tcagatcatc gctcgggaat
     5101 gcttacgggc gcgtggccca tcgggtccaa tcgcgtagct caccagcgtc ggaatccggc
     5161 gctattgcgg ataccgtagg cggtgaagcc ggcgtcgctc gtccgggcgc cgaggccgct
     5221 gaggcagaca aggagcgata gccgcgtgtc ttcctcgcgc cctcgcctgc acgtgcgact
     5281 tcattacgag ggagcgacgc gcccgagcct cattctggag gccggttcgc tgaatgtgct
     5341 cgacctctcg gagcgcgggc tgcgatatcg tcaggaattc ggccctgagc cggcgctagg
     5401 agccaagctg gaagggattt tgcgcctgac tagtggagaa acattgccgg ttcgaggaac
     5461 cgtggttcgg gtgatgcgtc ctgccaagcc ggaagacgcg agtgtcatcg aagtcgcggc
     5521 tcacctaacc gacgttggca ttccttcccg cgtaattctc tcggagcgga aggcactgca
     5581 agaaccgccg gcacactgag gccttcgccc acacgcgccc tttagcctgt cgagagcatc
     5641 ctgtctccca gccagccggt cgtccgcacg gtcatagtcg tcccgtgcta caacgaagct
     5701 gctcgattgg atgtgagccg cttccgcgaa tttgcggctg cgtcggcgga cgtgggcctt
     5761 ctgtttgtgg acgacgggag caccgataca acctcggcgg tgctgacgga gctggtccat
     5821 gaagtcccca accgcattcg cgggattcgg ctcgctgcca atcaaggcaa agccggtgcg
     5881 gtgcgggccg gcgtgctggt cgcgctcgcg agcgacccga cgttcgtagg cttcttggac
     5941 gccgacctgg caacgccgct ctcggcggtc aatcgcttcg tggccgccct cgaggctcgc
     6001 cccgagctgg tcggagttat tggtgcacgg gtcaaactgc tcggaacggt gatcgatcgt
     6061 tcggctgcgc gccactatct gggaagagtc ttcgcgacgg cggcctcact ggttctcaaa
     6121 ctccccgtgt acgacacgca gtgcggcgcc aagctcttcc gtgccggccc gctggtgcgc
     6181 gcggcgttcg gggcgccgtt tcggagtcgc tgggcgttcg atgtggagct cctggcccgg
     6241 ctgggtcagg cgggctcggg ggccgggccc cggccggcag ggaatctggt gatcgagttg
     6301 gcgctcgagg agtggcggga cgtgaaagga tcgaagctgg caccgcacgc gatgctgcgc
     6361 gcgggattgg atctggcctt tgtacgaagg aatgcgcgac gggatgcggg ccggagcctg
     6421 cctccgcccg catttacctg gctgtagaag atctcaggtc gtcagtacgc tctcgaagcg
     6481 ggcaaagtcg tccaggatcc tgccggtacc ccggaccacg caggtgagcg ggtcgggatc
     6541 ctgccgaacg gtcaagccgg tttcct
//
LOCUS       DASRJQ010000364         3323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1243361, whole genome shotgun sequence.
ACCESSION   DASRJQ010000364 DASRJQ010000000
VERSION     DASRJQ010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243361"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="VFB89_08125"
     CDS             <1..1010
                     /locus_tag="VFB89_08125"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZM27309.1"
                     /translation="RRVAADSRGWLVATLRGNQRLRHTQKGWEVQAVAAAPFSSSNAI
                     LIGRQQFRGTTCGLRPAIDTLPCLRRASKTGESPKTPLTIWFRRPVAVTDNIYIDQTY
                     RYGSTMGGNFQQHQGVEFNNPEGTPVLAIGSGTVVYAGRAEQGALTVAIRHDSTVTVR
                     GRRLAIWSTYYHNSKLDVKVGQRVAAGQRIARVGSTGRATNEHLHLEIHVAPADSARA
                     IVDSLERFPPYTTNPELWIEPLQGTGIIAGQVFDGAGNPVPQARIYGIIKPDPRETPF
                     SYAETYGDQAHSHPLYNEHFAVSDVPPGTYVLGTDIAGAKVMRRVTVEEGKLTWVVFK
                     P"
     gene            1062..1649
                     /locus_tag="VFB89_08130"
     CDS             1062..1649
                     /locus_tag="VFB89_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27310.1"
                     /translation="MYIELTDHLRCPGDHAEQYLVLLPDRIVDRSVISGRLGCPVCGR
                     TYPVTDGIAELGDSTVRPGAISSLDPAALHVLLGLSGPGGYAVLVGSTAARWERLARE
                     NPGVSLVAVNPPDTVADAGQLLSVLRAPLIPLKARSVRGVVLGPEFAADPEWVREAAR
                     VTLPGLRVVGQGRAPELDSLDLLATAEDCWVASKR"
     gene            complement(1698..>3323)
                     /locus_tag="VFB89_08135"
     CDS             complement(1698..>3323)
                     /locus_tag="VFB89_08135"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HZM27311.1"
                     /translation="VRAKDRVAILSENRPEWAIADYACLALCAADVPIYPTLPAKQIE
                     YILRDSGATVAFVSTQQQLAKIREIRAQLPALRHVVVFDESAATGDLLAFAGLSLRGR
                     AARAAAPNWRSEALGAKSDDIATIIYTSGTTGDPKGVMLTHGNITSNVVSCVSLLDIS
                     GTDECLSFLPLSHIFERMVGHYTMLHRGVTISYATSTETVAAEMAEIRPTIMAAVPRL
                     YEKIYARVLDAAVSGSPVKKRIFFWAKRNAETWVEYSLARRAIPASLAFKKRMGDRLV
                     FSKLRARTGGRVRFFVSGGAPLNPEIAKFFHAAGLPILEGYGLTETSPVIAVNTFKYL
                     KLGTVGRPIPGVEVRIADDGEILTRGPHVMVGYYNKPEATREAIDPDGWFHTGDIGIL
                     DGDGFLRITDRKKDIIVTAGGKNIAPQPIENMVKTSKFVSNCVMLGDKRKFPVMVVVP
                     NVDNLKAWASRHGFIAPDDRSLLALPEVTAKMDREVRKTLRDLAQFEVPKRLLLVASD
                     FSVESGELTPTLKVKRRVVERNYARQIEQLFHE"
BASE COUNT          576 a         1054 c         1072 g          621 t
ORIGIN      
        1 tccgccgggt cgcggccgat agccgagggt ggctggtcgc gacgctgcga ggcaatcagc
       61 ggctgcgaca cacccagaaa ggctgggagg tgcaggccgt cgccgcagct ccattcagct
      121 cctcgaacgc gattctgatc ggccgacaac aattccgagg cacgacctgc ggactcaggc
      181 cggcaatcga cacgctgccc tgtctccgtc gagcatcgaa gacgggtgag tctcccaaga
      241 cgccgcttac gatctggttc cggcgaccgg ttgcggtcac ggacaacatc tacatcgacc
      301 agacctatcg atacgggtcc acgatgggcg gcaacttcca gcaacaccag ggcgtggagt
      361 tcaataatcc cgagggcact cctgtcctgg ccatcgggag cgggacggtg gtctatgcgg
      421 gcagagcgga gcagggagcg ctgacggttg ccattcggca cgattccacg gtgacagtcc
      481 gcgggagaag actcgcgatc tggtcgacct actaccacaa cagcaagctc gacgtgaagg
      541 tggggcagag ggtcgccgcc ggccagcgta tcgcccgagt cggcagtacc ggtcgcgcaa
      601 ccaacgagca tcttcatctc gagatccatg tcgccccggc cgactcggcg cgcgcaatcg
      661 tggactcgct ggaacgcttt ccgccctata cgaccaatcc ggagctgtgg atcgagccgt
      721 tgcaaggaac cgggatcatc gcggggcagg tcttcgacgg cgccggtaat ccggtacccc
      781 aggccaggat ctacggcatc atcaagccgg atccgagaga gactccgttt tcctacgccg
      841 agacatacgg cgaccaggcg cattcccatc cgctctacaa cgagcacttc gcggtgagtg
      901 atgtgccacc gggcacctac gtactgggaa ccgacattgc cggggcgaag gtgatgcggc
      961 gggtcacggt tgaggaaggc aagctcacgt gggtggtttt caagccgtga actcggccca
     1021 cccccgttca ctgttcaccg ttcaccgttt acgctttatc ggtgtacatc gagctcactg
     1081 atcatctccg ctgtcccggc gatcacgccg agcagtatct cgttctcctg ccggaccgaa
     1141 ttgtcgatcg ttcagtaatc agcggccggc ttggctgtcc ggtctgcggc cgcacgtacc
     1201 ctgtgactga cgggatcgcc gagctgggag acagtacagt caggccggga gccatttcgt
     1261 cgctcgatcc ggccgccctg cacgtgctgc ttggtctgtc cggccccggc ggatacgctg
     1321 tgctggtcgg gagtaccgct gctcgctggg agcggttggc tcgggagaat ccaggtgtct
     1381 cgctggtcgc agtgaatccg cccgatactg ttgcggatgc gggtcagcta ctgagcgtgc
     1441 ttcgggctcc gctcatcccg ctcaaagcgc gcagcgttcg cggcgtcgtc ctgggccccg
     1501 agttcgctgc cgatcctgag tgggtgcggg aggctgcccg ggtcaccttg cccggattgc
     1561 gggttgtcgg gcagggcagg gcgccggagc tggattcgct ggatctattg gcgacggcag
     1621 aagactgctg ggtggcgagc aagcggtaga aggggcaggc gggccgtctc aatcgaccgc
     1681 cccggattga cggcgaccta ctcgtggaac agctgctcga tctgccgagc atagttccgc
     1741 tcgaccaccc gccgcttcac cttgagcgtg ggcgtcagct ccccgctctc cacgctgaag
     1801 tcgcttgcca cgagcagcag tcgcttggga acctcgaact gggcgagatc ccggagcgtc
     1861 ttgcggacct cccgatccat tttggccgtc acttcgggga gtgccagcag gctgcggtcg
     1921 tccggggcga tgaagccgtg tcgcgatgcc cacgccttga ggttgtcgac gttcggtact
     1981 acgaccatca ccgggaattt ccgcttgtct cccagcatga cacagttgga gacgaatttg
     2041 ctcgtcttca ccatgttctc gatcggctgc ggcgcgatgt tcttgcctcc ggcagtcacg
     2101 atgatgtcct tcttgcggtc ggtgatccgc agaaagccat cgccgtcgag gatgccgatg
     2161 tcgcccgtat ggaaccatcc gtccgggtcg attgcttccc gcgttgcctc cggcttgttg
     2221 tagtagccga ccatcacgtg cggtccacgg gtcaggatct cgccgtcgtc ggcaatgcga
     2281 acctcgacgc ccggtatcgg ccgtcctacc gttcccagct tgaggtactt gaacgtgttg
     2341 accgcgatga ccggcgacgt ctcggtgagc ccgtagccct ccagaatggg gagccccgcc
     2401 gcgtgaaaga acttggcgat ttcaggattg agcggcgccc ctcccgacac gaaaaagcga
     2461 acgcgaccgc cggtgcgggc gcgaagcttg gagaagacca ggcggtcccc cattcgcttc
     2521 ttgaaagcca gcgaagcagg aatggcgcgg cgagccaggc tgtactccac ccaggtctcg
     2581 gcgttgcgct tggcccagaa gaagatccgc ttcttgaccg gtgatcccga gaccgccgca
     2641 tccagcactc tcgcatagat cttctcgtag agccgcggca cggcggccat gatcgtcggc
     2701 cggatctcgg ccatttccgc cgccacggtt tcggtgctgg tggcgtagct gatcgtgacc
     2761 cctcgatgca gcatcgtgta gtgccccacc atgcgctcga agatgtgcga cagcggaaga
     2821 aacgagagac actcgtccgt tccggaaatg tcgagcagac tcacgcacga gacgacattg
     2881 gacgtgatgt tcccgtgggt gagcatcacg cccttggggt cgcccgttgt tcccgatgtg
     2941 tagatgatcg tggcgatgtc gtcggacttg gcaccgagcg cctcgctccg ccagttaggt
     3001 gcggcggcgc gcgccgcccg tccccggagg gacagtcccg cgaaggccag caggtcgcca
     3061 gtcgcagccg actcgtcgaa cacgacaacg tgccgaagag ccggcaactg ggcccgtatc
     3121 tcccggatct tggcgagttg ctgctgcgtc gagacgaacg cgaccgtcgc cccggagtct
     3181 cgcaggatgt attcgatctg cttggcgggg agggtcgggt agatcggcac gtcggcggcg
     3241 cagagagcga ggcacgcata atctgcgatg gcccactccg ggcggttttc cgagaggatc
     3301 gccacccggt ctttcgcccg gac
//
LOCUS       DASRJQ010000365         6963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3548992, whole genome shotgun sequence.
ACCESSION   DASRJQ010000365 DASRJQ010000000
VERSION     DASRJQ010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6963
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3548992"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VFB89_08140"
     CDS             complement(<1..486)
                     /locus_tag="VFB89_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27312.1"
                     /translation="MSRRYPFSLSPLLLFSLLCLPTAALSGQQSISKQLEQSQRRLEE
                     IKAERDRLQQQRERLQGQVHDVNDELTNLERQKESTQRIVNEIERQIGGLAGQLDHAS
                     AELILAEDNLAERRAILERRLVDIYKRGALYAFQVLLTAESFGDLLSRYKYLYLTSRQ
                     DR"
     gene            complement(527..1396)
                     /locus_tag="VFB89_08145"
     CDS             complement(527..1396)
                     /locus_tag="VFB89_08145"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HZM27313.1"
                     /translation="MRLLFREALLSFRRAPLLSGLSVTTIAFSLFTLGLFLLVAVNLR
                     EALKGLEERVEIVAFVLRGTPPETITLASQDIAAFPEVQAVDYVTEEQALARARKELV
                     EFRDAYRDLSVNPLPASFEIRLKEGYRNAAAAERVAKRLEGFSFVEDIRYGRDWVERL
                     DKLRNLAGIVGLVIGAAFAAVAVVIIGVTIRLTIMQRAREISIMRLVGATNRFIRSPF
                     LLEGALKGLLGGLLSLALCYAGFRLFRDSNLGTLGGLIFFGPDQMAMLVVFGVLLGLF
                     GSLVSVGRHLRKV"
     gene            complement(1393..2085)
                     /gene="ftsE"
                     /locus_tag="VFB89_08150"
     CDS             complement(1393..2085)
                     /gene="ftsE"
                     /locus_tag="VFB89_08150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HZM27314.1"
                     /translation="MIRFHNVFKSYPNGALALKDVTFHVSKGEFVFLTGHSGAGKSTI
                     MKLLFAEQRPSQGDVRVSGFAVSSISSSDIPRLRRRLGIVFQDFRLLEDRTASENVAF
                     ALEVTGARRDTIPARVMRVLTQVGLAAKGRAYPRELSGGEQQRVAIARALVNDPAILL
                     ADEPTGNLDERATRGVFQLLREINAGGTVVVMATHDLDLVRQAPYRTIEMRDGGVVYD
                     SAEDEPSEEATS"
     gene            complement(2149..3297)
                     /locus_tag="VFB89_08155"
     CDS             complement(2149..3297)
                     /locus_tag="VFB89_08155"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HZM27315.1"
                     /translation="MSGRRGLSTTAVHGVIHRRAEWSPVAPAIVQSSTYINPVGSDEP
                     VLYAGDGNTPTQLAVAQKYAALEGAEAAVMVASGMAATALAHLAVLRPGDHLISSRWI
                     YGGTQRLFDEELARFGIGITYVSPDEPRLWRKSVRKETRAIFVETPTNPLIRVVDLQP
                     ILKIAKEFGLALLVDGTFASPMNFRPLEHGADVVITSATKYLNGHSDVIAGAVAGSAS
                     FVDEVSRLMRLWGQAIDPFAAWLLDRGLRTLSVRIERHNANGLAVAQWAAKQKWIDKV
                     HYPGLRAHPDHTVAARVLGGFGGMIGLQLSGGPPAAERFLSRLRVVIHAPSLAGVESL
                     ISEPRLTSHVHLSPEQRATLGFPDGFVRLSCGIEDADDIIADLEQAGR"
     gene            complement(3294..3833)
                     /locus_tag="VFB89_08160"
     CDS             complement(3294..3833)
                     /locus_tag="VFB89_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27316.1"
                     /translation="MVQTISLHHVLQEAVATPYRNLVTRATGIAVRGRIEATLATGGG
                     HTALLDFSGVDCLDMSCADEIVAKLLLAAASGDRVLVLTGVREEQREALEHVLAHHHL
                     AVAACFRGSDAPDVLGWISGDAREAFADLRRRGSTDVLGLAARMTWPVQRAAVALDDL
                     AGHRIAQPIAGVYHVVTVS"
     gene            complement(3833..4732)
                     /locus_tag="VFB89_08165"
     CDS             complement(3833..4732)
                     /locus_tag="VFB89_08165"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZM27317.1"
                     /translation="MTRVVEVPAHFDDRSFDQFAAGFDTWPTEERVLLDARATQWASP
                     YGLVGLLTAGQALSEARKERPLLTVPASDEVRRYWTRTGFFAHAAELFEVHGKVPRAA
                     ATGPSDVLLDVTPIRASEDIHEIVGKIQENAARILTGELGIEAKATMGFAMALSEACQ
                     NIVEHAGTSGWVAVQAYTFRRRLGRRVVVIAVSDAGVGFRRSLESTQARRFGDRWGDG
                     SALEAALIQNVSRFRDPGRGQGLAGIKRYLSRWQGKISIRSGTARLAIVPAWDDDVPL
                     VDHLPYFPGAQVQVTIPAQNAEP"
     gene            complement(4729..5688)
                     /locus_tag="VFB89_08170"
     CDS             complement(4729..5688)
                     /locus_tag="VFB89_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HZM27318.1"
                     /translation="MKKVGVVGSLVWDEIHGRDPAAPPVEEWGGIAYALGGLDAALPD
                     DWEIVPLIKVGRDLAPQAGEFLRSLRRVAPGARFVEVPVPNNRVVLYYESADRRCERM
                     SGGVPAWTWPELGPMVRDLDAVYLNFISGFEMCLGTAQALRHGFHGPIYADVHSLFLG
                     MQQDGIRVLRPLPDATDWFACFDVVQVNEDEMRQLSPEPMGMAAQVLGAGVSLLAVTL
                     GPRGAAYVATAGFHRWERGGKGEGENPSSPARTALIPAPAVDALDPTGCGDVFGAVLC
                     SRLLAGEDVEAALREANRLAARNATFRGAGGLSRFLRGELVTA"
     gene            complement(5685..6662)
                     /locus_tag="VFB89_08175"
     CDS             complement(5685..6662)
                     /locus_tag="VFB89_08175"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZM27319.1"
                     /translation="MRFVLGVDVGGTNLVVAAVAADGSTLHALKSEATRPEEGSESVL
                     HRMVTMAHAVMEETRRAIPKAEFIGAGVGAPGPLDTKRGVVLLTPNLGWVNLPLRDLV
                     QQGISLPTAIDNDANCAVLGECWMGAGRGALNVIGITIGTGIGGGIVIDGRLYHGSSD
                     SAGEIGHTTVEVNGRRCKCGNYGCLEAYASGPAIALRAVEAIEAGQPSRLPEYVNGAL
                     GKITAQTVYQAAHDGDELADEVVGDTAKFLGAGIANMINIFNPEIVVVFGGVTYAGER
                     LFAPLRREVAKRAFKPAVAVCRIVPAELIGTAGVYGAARTFLDQRDARA"
     gene            complement(6702..>6963)
                     /locus_tag="VFB89_08180"
     CDS             complement(6702..>6963)
                     /locus_tag="VFB89_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683641.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HZM27320.1"
                     /translation="KVAEKLKELIPRQLYQVVIQAAIGNKIIARETISAFRKDVLAKC
                     YGGDVTRKRKLLEKQKEGKKRMKQIGAVEIPQEAFLAVLQVE"
BASE COUNT         1243 a         2343 c         2192 g         1185 t
ORIGIN      
        1 tcgatcctgg cggctggtga gatagagata cttgtagcgg gaaagcagat cgccgaacga
       61 ctcggcggtg agcagcacct ggaatgcgta gagcgcaccg cgcttgtaga tgtccaccag
      121 ccgccgctcc agaatcgcgc gacgctcggc caggttgtct tcggccagga tcaactccgc
      181 gctcgcgtgg tccagctggc cggcgagccc accgatctgc cgttcgatct cgtttacgat
      241 gcgctgagtg gattcttttt gtcgctccag gttggtcagc tcgtcgttga cgtcgtggac
      301 ctgtccctgg agtcgctccc gctgctgctg cagccgatcg cgctcggcct tgatctcctc
      361 caggcgtcgc tgactctgtt cgagctgttt gctgatgctt tgttgcccgg acagagcggc
      421 ggtgggcagg cagagaaggg agaaaaggag aaggggggaa agggagaatg ggtaccgcct
      481 gctcaccccc attctcccat tctcccattc tccctttccc cgcatgtcac acctttctca
      541 ggtgccgacc cacgctcacc aggctcccga agaggccaag gagcacacca aagacaacga
      601 gcatggccat ctggtccgga ccgaagaaga tcagcccgcc gagcgtgccc aggttgctgt
      661 cgcgaaataa tcggaagccg gcatagcata gagcgagcga cagcaatcca ccgagcaggc
      721 ccttgagcgc gccctcgagc aggaaggggc ttcgaatgaa tcgattcgtc gctcccacca
      781 ggcgcatgat gctgatctcc cgggcgcgct gcatgatcgt gagccgaatc gtcacgccga
      841 tgatcaccac cgcgaccgcc gcgaacgccg caccgatgac cagcccgaca atgccggcca
      901 ggttcctcaa cttgtcgagc cgttcaaccc agtcgcggcc gtaacggatg tcttctacaa
      961 agctgaagcc ctccagccgc ttcgctactc gctcagcggc cgcagcgttg cgatatccct
     1021 ccttgagccg gatctcgaat gaggccggca gcgggttgac cgagaggtcg cggtaggcat
     1081 cgcggaactc gaccagctct ttccgtgccc gggccagcgc ctgctcttcg gtcacgtagt
     1141 ccaccgcttg gacttcggga aaggcggcga tgtcctggga ggcgagtgta atggtttcgg
     1201 gcggcgtgcc ccgcagcacg aaggcgacaa tctccactcg ctcctcgagc cctttgagcg
     1261 cctcgcggag attgaccgcg accagcagaa aaagaccgag ggtaaagagc gagaacgcga
     1321 tcgtcgtgac cgaaagcccc gagagcaggg gagcccgcct gaacgaaagc agcgcctcgc
     1381 gaaacaagag tctcacgagg tggcttcctc ggaaggctcg tcctcggcgc tgtcgtagac
     1441 gacgccgcca tcacgcatct cgatcgtgcg gtagggtgcc tggcggacca ggtcgaggtc
     1501 gtgtgtggcc atgacgacga ctgtgccgcc tgcgttgatc tctcgcaaca actggaagac
     1561 gccgcgggtc gcccgctcgt cgagattgcc ggtcggctcg tcagccagga ggatggccgg
     1621 atcgttgacc agcgcccgcg cgatcgccac tcgctgctgc tcgccaccgg acagctcgcg
     1681 cggataggcc cggcctttgg ccgcaagacc gacctgggta agcacccgca tcacgcgggc
     1741 gggaatggta tcgcgccgcg ctccagtcac ctccagtgca aaggcgacat tctcggacgc
     1801 ggttcgatct tccagcagcc ggaaatcctg aaacacgata ccgagccggc ggcgcagtcg
     1861 aggaatgtca ctgctgctga tcgacgacac agcgaagccg gagacccgta catcaccctg
     1921 gctcggacgc tgctcggcaa agagcagctt catgatggtg gacttgcccg cgccgctgtg
     1981 accggtcaga aacacgaact cgcccttgga gacgtggaag gtgacgtcct tcagagcgag
     2041 agcgccgttg gggtaggact tgaagacgtt gtggaagcgg atcacggggg agaaagataa
     2101 gtaaagtcag catgcgctgg cagctatcag ctatcagctg tcagctccct accgccccgc
     2161 ctgctccaag tccgcgataa tgtcatccgc atcttcgatc ccgcagctca gtcgcacaaa
     2221 cccatcggga aatcccagcg ttgcccgctg ctccggcgag agatgcacgt gcgacgtaag
     2281 gcgtggctcc gagatcaggc tctccactcc cgcgaggctc ggcgcatgga ttaccacccg
     2341 cagccgtgat aggaaccgct cggccgcggg tgggccgccg gagagttgga gccctatcat
     2401 tccgccaaat ccgccgagca ctcgcgcggc gacggtgtga tcgggatggg cccggaggcc
     2461 ggggtaatgg accttgtcaa tccacttctg cttcgctgcc cactgagcga ccgcgagtcc
     2521 gttggcgttg tggcgctcga tgcgaaccga gagcgttctg agtccgcgat cgagcagcca
     2581 ggccgcgaag gggtcgatgg cctgccccca cagccgcatc agccggctga cttcgtcgac
     2641 gaacgaggcg gagccggcga ccgcgcccgc gatgacgtca ctgtgcccgt tgaggtactt
     2701 ggtggcgctg gtgatgacga cgtcggctcc gtgctcgagc ggccgaaagt tcatcgggct
     2761 ggcgaaagtg ccgtccacca gcagcgccaa cccgaactct ttggcaatct tgagaatcgg
     2821 ctggagatcg acgacccgga tcagcggatt ggtcggcgtc tctacgaaga tggcccgggt
     2881 ctccttgcgg acgctcttac gccacagcct gggctcgtcc gggctcacat aggtgattcc
     2941 gatcccgaac cgggctagct cttcgtcgaa gagccgttga gtgccgccgt agatccagcg
     3001 cgatgaaatg aggtggtccc ccggacgaag caccgcgaga tgcgcgagcg ccgttgccgc
     3061 cattccactc gcgaccatga cagcggcttc cgcgccctcg agagctgcgt acttctgggc
     3121 gaccgcgagt tgggtaggag tgttcccgtc gccggcgtag aggacaggct cgtcggagcc
     3181 gacgggattg atgtaggtgg agctttggac gatcgcgggc gcaaccggag accactcggc
     3241 ccggcgatgg atcactccat gaacggcggt ggttgagagt cctcgccgac cgctcacgaa
     3301 acggtcacga cgtggtacac cccggcgatc ggctgtgcga tgcggtgacc ggcgaggtcg
     3361 tcgagcgcga cggcggcccg ctgaaccggc caggtcatgc gggcggcgag gcccaatacg
     3421 tcagttgagc cgcgccgacg cagatcggcg aacgcctcac gggcgtcacc gctgatccag
     3481 ccgagcacgt cgggggcatc gctgcccctg aagcacgcgg ccaccgcgag atgatggtgc
     3541 gccagcacgt gctccaatgc ctcgcgctgc tcttccctca cacccgtgag caccagcacg
     3601 cgatcgccgc tggcagcggc gagcagcagc ttggcgacga tctcgtcggc acaactcatg
     3661 tcgaggcagt cgacaccgga aaaatcgagc agcgcggtgt gacccccgcc tgttgccagg
     3721 gtcgcttcga tcctcccccg gaccgcgatt cccgtggcgc gggtgaccag gttgcggtac
     3781 ggggtcgcca ccgcttcctg caggacgtgg tgcaggctga tcgtctgaac catcagggct
     3841 ccgcgttctg ggcgggaatg gttacctgca cctgggctcc agggaaatag ggaaggtgat
     3901 caaccagcgg cacgtcgtcg tcccatgccg gcacgatggc gagacgtgcg gtaccgcttc
     3961 ggatgctgat cttgccctgc caccgcgaca agtaccgctt gatgcccgcc agtccctgtc
     4021 ctcgtcccgg gtcgcgaaag cggctgacat tctgaatcaa agcggcttcg agcgcgctgc
     4081 cgtcgcccca acggtctcca aagcggcgag cctgtgtgct ttcgagggag cgtcggaacc
     4141 cgacgccggc gtcgctgact gcgattacga caacccgacg accgagccga cgacgaaagg
     4201 tatacgcttg taccgccacc cagccactgg tgcccgcgtg ctcaacaata ttctggcatg
     4261 cctccgaaag cgccatggcg aatcccatgg tcgccttcgc ctcgatgccg agctcgccgg
     4321 tcagaattcg tgctgcgttc tcctgaatct tgccgacgat ctcatggatg tcttccgacg
     4381 cgcgaatcgg tgtcacgtcc agcaacacgt ccgacggtcc cgtggcagcg gctcggggca
     4441 ccttgccgtg cacctcaaaa agctcagccg cgtgggcgaa gaagccggtc cgggtccagt
     4501 agcgcctcac ttcgtcgctg gccggcaccg tcagcagcgg gcgctcttta cgtgcctccg
     4561 acagtgcctg accggccgtg agaagcccga ccagtccgta aggcgaggcc cattgggtcg
     4621 cgcgagcgtc caggagcacg cgctcttcgg tcggccaggt gtcgaagccc gcggcgaact
     4681 gatcgaagct tcgatcgtcg aagtgggccg gtacctcgac gaccctggtc acgcggtgac
     4741 cagctcaccg cgcagaaacc gcgagagccc acccgctccg cggaacgtag cgttgcgagc
     4801 cgcgagccgg ttcgcctcgc gcaacgcggc ttccacgtcc tccccggcca gcagccggga
     4861 gcacaggacc gcgccgaaga cgtcgccgca acccgtgggg tcgagcgcgt cgacggcggg
     4921 cgcagggatg agagcggttc gcgcgggaga agaggggttc tccccttctc cctttccccc
     4981 cctctcccac ctgtggaatc ccgcagtcgc aacatacgcc gccccccgcg gccccagcgt
     5041 caccgcgaga agcgacaccc ccgcgccgag cacttgcgca gccataccca tcggctccgg
     5101 cgaaagctgc cgcatctcgt cttcgttcac ctgcaccacg tcgaagcagg cgaaccagtc
     5161 ggtcgcgtcc ggcagcgggc gcagcactct gatgccgtcc tgctgcatgc cgagaaacag
     5221 gctgtgcacg tcagcgtaaa tcggtccgtg aaacccgtgc cgcagtgcct gcgccgtgcc
     5281 aagacacatt tcgaagccgg agatgaagtt caggtaaacg gcatcgagat cccgcaccat
     5341 gggcccgagc tcgggccagg tccacgcggg aacacccccc gacatccgct cgcaacggcg
     5401 atcggccgat tcgtagtaca gcaccacccg attgttgggc accggcactt cgacgaaccg
     5461 ggcgcccggc gccactcggc ggagcgagcg cagaaactcg ccggcctggg gtgcgagatc
     5521 gcggcccacc ttgatgagcg gcacgatctc ccaatcgtcc ggtagcgctg cgtccaggcc
     5581 gcccaacgcg tacgcgatgc cgccccactc ctcgaccggc ggcgccgccg ggtcgcgccc
     5641 gtgaatttcg tcccacacga gggagcccac cacgccaact ttcttcaagc gcgagcgtcc
     5701 cgctggtcga ggaaggttcg agcggcgcca tagacgccgg cggtgccgat cagctccgcc
     5761 gggacgatcc ggcacaccgc caccgcgggc ttgaacgcgc gtttggccac ttcacggcgg
     5821 agcggagcga acaagcgttc gccggcgtag gtcactccgc cgaagacgac cacgatttcc
     5881 ggattgaaga tgttgatcat gttggcgatg ccggcgccga gaaacttcgc agtgtcgccc
     5941 accacctcgt cggccagctc gtcgccgtcg tgcgcggcct ggtacacggt ttgggctgtg
     6001 attttcccca acgcgccgtt cacgtattcg gggaggcggc tgggctggcc tgcttcgatc
     6061 gcttcgaccg cgcgcagtgc aatcgccggc ccggaggcgt aggcctcgag acagccatag
     6121 ttgccgcact tgcagcgtcg cccgttcact tcgacggtgg tgtggccgat ctcgccggcg
     6181 gaatcggatg agccatggta cagacgtcca tcgataacga tcccgccacc gatacctgtc
     6241 ccgatggtaa tcccgatcac gttgagtgcg cctcggcctg ctcccatcca gcactcgccc
     6301 agcacggcgc agttggcgtc gttgtcgatc gcggtcggga gcgaaattcc ctgttgcacc
     6361 aggtcgcgca acgggagatt gacccatccc agattgggcg tgagcagaac gactccgcgc
     6421 ttggtgtcga gcggccccgg cgccccgacc ccggcgccga tgaactctgc cttcgggatc
     6481 gcccgccggg tttcctccat gactgcgtgc gccatcgtga ccatccggtg gagcaccgac
     6541 tcggaccctt cctccgggcg ggtagcctcg ctcttgaggg cgtgaagagt agagccgtcg
     6601 gctgcgaccg cagcgacgac caggttcgtg cccccgacgt caacgcccag gacgaatcgc
     6661 accacgctcc tccactgaat ggacgacggt gcgccggtcg atcattctac ctggagcacc
     6721 gccagaaacg cctcctgcgg gatctcgacc gccccgatct gcttcatccg cttcttaccc
     6781 tctttctgtt tttccagaag cttgcgcttg cgggtgacat cgccgccgta gcatttggcg
     6841 agcacgtctt tccggaaggc cgagatagtc tccctcgcga tgatcttgtt gccgatcgcc
     6901 gcctgaatta ccacctggta gagctgccgc gggatcagct ccttgagctt ctccgccacc
     6961 ttg
//
LOCUS       DASRJQ010000366         4508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3773583, whole genome shotgun sequence.
ACCESSION   DASRJQ010000366 DASRJQ010000000
VERSION     DASRJQ010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3773583"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..114
                     /locus_tag="VFB89_08185"
     CDS             10..114
                     /locus_tag="VFB89_08185"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HZM27321.1"
                     /translation="MLGVTRERVRQLRDRALKRLREGEVGRALASFAA"
     gene            155..916
                     /locus_tag="VFB89_08190"
     CDS             155..916
                     /locus_tag="VFB89_08190"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM27322.1"
                     /translation="MLRAVATHAAQPRTFVTRLGHFGTEAGRALADVRTWRPLLLGQM
                     RRLGVDSIPIAIFIAIFTGIVLSLLSSYIFTGAVPLYFVGALVGKTVMMELGPVLTGM
                     ALAGRVGANIAAELGTMRVTEQVDALETLAYDKYAYLVVPRVIAATLMFPIVTSFAMA
                     VGVASGWITSTHVLDLSTPEFLKGLKLFYLFKDIWFGLVKSASFGTAVALMGCIEGLN
                     TSGGAEGVGRSTTRAVVLGCQSILVLDAFWALVLL"
     gene            913..1668
                     /locus_tag="VFB89_08195"
     CDS             913..1668
                     /locus_tag="VFB89_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZM27323.1"
                     /translation="MIRLEGVYKSFGPKVVLAGLSLQVPAGMNTVIIGGSGSGKSVTL
                     KLIVGLLEPDAGRIEVDGRNVPDLDRDDLAQLRGQIGYVFQFAALFDSMTVAENIRLG
                     LVKRGLDEGSIRDRIRESLDTVELSGQEDRFPAELSGGMRKRAGIARAVALRPRYILY
                     DEPTTGLDPVTSAVMDRLMQRTRDLGVTGVVVTHDMRSAFTVGDRIAMLHDGVIRQVG
                     TVAEIKATSDPIIRQFIEGRPDIEAPPLRRAAQ"
     gene            1665..2672
                     /locus_tag="VFB89_08200"
     CDS             1665..2672
                     /locus_tag="VFB89_08200"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HZM27324.1"
                     /translation="MKRGNEFAVGLAVLASIALVIGGALWLGNAHIGKRPMTHVARFR
                     TVGALGVGAPVTLRGVKVGQVQAVKLSEDGWVDADLTIDNDVALPRRPAVISASSSLF
                     GEWTAEIIPFEPLPDDPNIRSALLESDATPGDAWPGATLPDIGQLTAQASRIAGDVAD
                     VTQRIRGVFDTAAVRDVRRSVADLTAIADRLAKFTNQQADRLDRVSASVATSADAFAG
                     VARGFERTIARVDSATNANQFQEIMNNARASSADLRQAASDFRSVMTAARENQASLVH
                     VIQSADSLISRLHSGEGSLGRLTTDTALYVETRRAVVELRELLADVKANPKRYFKVSV
                     F"
     gene            2672..3154
                     /locus_tag="VFB89_08205"
     CDS             2672..3154
                     /locus_tag="VFB89_08205"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZM27325.1"
                     /translation="MGKRRAELEVSAGGIVYRRQPDAGTQFLLIKDSYDNWGFPKGHL
                     EDGETPAEAASRETAEETGLDHLVLQGPIRVIDWHFRFRGRHIHKYCHFFLFESVTGE
                     PAPQVEEGITACRWETLDQALEALSYDNARGVLKRAGEMVRTLIATGVGRSRPSPDGA
                     "
     gene            3257..3928
                     /locus_tag="VFB89_08210"
     CDS             3257..3928
                     /locus_tag="VFB89_08210"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZM27326.1"
                     /translation="MARLRRTLELRLVDAIVVAPNPVLMPEVAGLRQGFPAIPVVAYA
                     PFRPDDGELLVDCERHEIAEFAIEGVDDPVIGDLVVRCSLTTARRRALADGPRMLRLS
                     DSLQQRAWHYLVTEVERPVRTLTLARQLAVSREHLSRQFGAGGAPNLKRVIDLTRVAC
                     AAQLLRNPGYRIATIVRLLHFASSSHLAGTVRRIASVAPSALGTLGPKGVLAAFARGN
                     TRSRW"
     gene            4040..>4508
                     /locus_tag="VFB89_08215"
     CDS             4040..>4508
                     /locus_tag="VFB89_08215"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682203.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HZM27327.1"
                     /translation="MATDTTLRPGELKEVLLREIAEADVAAQDTSEVGTVLEVRDGVA
                     RIYGLRSAEAGEMLEFTAQESGEVVTGLVLNLEEDNVGAAIMGDYLKLKEGDEVRRTR
                     RLLEVPSGSAMLGRVVDALGRPVDGRGEIKASASRPVEMIAPGIIVRQPVKEPL"
BASE COUNT          730 a         1476 c         1481 g          821 t
ORIGIN      
        1 atcggcggca tgcttggcgt cacccgcgag cgggtacgcc agctccgcga ccgcgcgctc
       61 aagcgcctgc gtgagggcga ggtcggccgg gcgctcgcaa gctttgccgc ctaagcggat
      121 agcggatcgc ggatagccaa cgtagcttct gttcgtgcta cgcgctgtag cgacccacgc
      181 cgcccagcct cgcaccttcg tcacccggct cggtcacttc ggcaccgagg cggggcgcgc
      241 actcgccgac gtgcgcacct ggcgcccgct cctgctgggc cagatgcgcc gcctcggcgt
      301 cgattcgatc cccatcgcca tcttcatcgc catcttcacc ggcatcgtcc tatcgctgct
      361 ctcgtcgtac atcttcaccg gtgccgttcc cctctatttc gtcggcgcgc tggtcgggaa
      421 gacggtgatg atggagctgg ggccggtgct caccggaatg gcgctggcag gacgcgtcgg
      481 ggcgaacatc gccgcggagc tcggcaccat gcgcgtgacc gagcaggtcg atgcgctcga
      541 gacgctggcc tatgacaagt acgcctacct cgtggtgccc cgcgtcattg cggccacgct
      601 catgtttccc atcgtcacct cgttcgcgat ggctgtgggc gtcgcctccg gatggatcac
      661 gtccacccac gtactggacc tctccactcc cgagttcctg aaggggctca agctcttcta
      721 cctcttcaag gacatctggt tcggcttggt gaagagcgcc agctttggca ccgccgtcgc
      781 actgatgggc tgcatcgaag ggctgaacac cagcggtggg gctgagggtg tcgggcgctc
      841 cactacgcgg gccgtcgtgc tcggctgcca atcgattctg gtgctcgacg cgttctgggc
      901 gctggttctg ctttgatccg gctggagggc gtttacaagt cgttcggacc caaggtcgtg
      961 ctggccggcc tctcgctcca ggttcccgcc ggcatgaaca ccgtgatcat cgggggttcg
     1021 ggcagcggca agagcgtcac gctcaagctg atcgtcggat tgctggagcc ggatgccggc
     1081 cgcatcgaag tcgatggccg gaacgtgccc gacctcgatc gtgacgattt ggcccagctc
     1141 cggggtcaga tcggatacgt cttccagttc gccgcgttgt tcgactcgat gacggtggcg
     1201 gaaaacattc gtctcggcct ggtcaagcgg gggctcgacg agggctccat tcgcgaccgg
     1261 attcgggaga gcctcgacac tgtggagttg agcggccagg aggatcgctt tccggcggag
     1321 ctgtcgggcg gaatgcgcaa acgcgccggc atcgcacgag ccgtcgctct ccggccccgc
     1381 tacattctgt acgacgagcc caccaccggg ctcgatcccg taacatccgc ggtcatggac
     1441 cgcctcatgc aacggacccg cgatctcggg gtcaccgggg tggtcgtgac ccatgacatg
     1501 cgcagcgcgt ttacggtcgg ggatcgcatc gccatgctcc acgatggagt gatccgccag
     1561 gtcggcacgg tggcggaaat caaggcgacc agcgatccga tcatccgaca gttcatcgag
     1621 ggtcggcccg atatcgaggc gccgcccctt cggagggccg cccagtgaaa cgaggaaacg
     1681 agttcgcggt cgggttggcg gtgctcgcct cgattgcgct ggtgatcggt ggcgcgctct
     1741 ggcttggcaa tgctcacatc ggaaagcgac cgatgacgca cgtggctcgc ttccggacgg
     1801 tcggcgcatt gggtgtcggg gcgccggtca cgcttcgcgg cgtcaaagtc ggacaggtcc
     1861 aggccgtgaa gctcagcgag gatggctggg tggatgccga cctcaccatc gacaacgacg
     1921 tcgccttgcc tcgtcggccg gctgtcattt ctgcatccag cagcctgttc ggcgagtgga
     1981 cggcggagat catccccttc gagccgctgc cggacgatcc caatatccgc tccgcgctgc
     2041 tcgagtccga tgccaccccc ggcgatgcct ggcccggcgc cacgttgccc gacatcggtc
     2101 agctgaccgc gcaggccagc cgcatcgccg gtgacgtggc cgatgtaacc caacgcatca
     2161 ggggggtctt cgatacggcg gccgttcgcg acgttcgtcg cagcgtcgcg gatctcaccg
     2221 ccatcgccga ccgtctcgcc aagttcacca atcagcaggc cgaccgtctc gatcgggtca
     2281 gcgcgagcgt cgcgacctcc gccgacgcct ttgccggcgt ggccagggga ttcgagcgca
     2341 ccatcgcccg ggttgatagc gccaccaacg ccaatcagtt ccaggagatc atgaacaacg
     2401 cgcgagcatc gagcgccgat ttgcgccagg cggcctccga ttttcgctcg gtcatgacag
     2461 cggcccggga gaatcaggcc agtctggtgc acgtgatcca gtcggccgat tccttgatca
     2521 gtcggcttca cagtggcgaa gggtcgctcg gacggctcac tacggacaca gccctctacg
     2581 tcgaaacgcg acgggccgtg gtcgagctgc gcgagctgct ggccgacgtc aaggcaaacc
     2641 cgaagcgcta cttcaaggtc tcggttttct gatggggaaa cgacgagccg agctggaggt
     2701 aagcgccggg gggatcgtct accgccggca gcccgatgcc ggtacccagt ttctcctgat
     2761 caaagactcg tacgacaact ggggttttcc caaaggacat ctggaagacg gggagacccc
     2821 ggcggaagcg gcgtcgcgcg agaccgcgga agaaaccggc ctggatcacc tggtgcttca
     2881 gggacccatt cgagtgattg attggcactt ccgctttcgc ggccgccaca tccacaagta
     2941 ttgccacttt tttctgttcg agtcggtcac cggcgaaccg gctccccagg tcgaggaagg
     3001 catcacagcc tgccgctggg aaacgctgga tcaggccctt gaagcgctct cctacgacaa
     3061 cgcccgcggc gtgctcaagc gggccggcga aatggtccgc accctgatcg ccaccggtgt
     3121 gggccgatcg cgcccctctc ctgacggcgc ctgacccgtg ccggtggtcg cgacgttgct
     3181 cgagagtcga gcagcttcgc tcgccctccg gcgaagcctg cccaggggcg gtcccggaat
     3241 agtcagctgt cgaaccatgg ctcgcctccg gcggaccctc gagctccggc tggtggatgc
     3301 gatcgtagtt gccccgaatc ccgttctcat gccggaggtt gccggcctcc gccagggctt
     3361 tcctgccatt ccggtggtag cctatgcacc gttccggccg gacgacggcg agctcctggt
     3421 cgactgcgag cggcacgaga ttgccgagtt cgcaatcgag ggtgtggacg acccggtgat
     3481 tggcgacttg gtggtgagat gttcgctcac cacggcgcga cggcgagcgc tggcggacgg
     3541 gccgcggatg ctgcgcctgt ccgattcgct ccagcagcga gcctggcatt atctggtaac
     3601 cgaggtggag cgcccggtcc gcaccctcac gctcgcgcgg caactcgcag tgagccggga
     3661 gcacctctcc cgtcagttcg gggcgggagg agctccgaac ctgaagcggg tcatcgatct
     3721 cacccgggtg gcctgcgcgg cgcagctgct tcggaacccc ggctaccgga tcgccacaat
     3781 cgtgaggctg ctccatttcg catcgtcgag ccatctcgcg gggaccgtgc gtcgcatcgc
     3841 aagcgtggct ccgtcggcct tggggacctt gggccccaag ggcgtcctgg cggcttttgc
     3901 gcggggaaac acacggagca ggtggtaacg gagcagccgg taacgttgta tcatctgtac
     3961 tcttgttggt gccccgcaat tgtgattaat ttcacaagct cggcggtagc ccgtcttttt
     4021 cacctcgtct cgatgaccaa tggccactga cacaactctc cgtcccggtg agctcaagga
     4081 agtcctgctc cgggaaatcg cggaagccga tgtcgctgcg caggacacct ccgaagtcgg
     4141 tacagtcctc gaggtacgcg acggtgtcgc ccgcatctat ggcctcaggt ccgccgaggc
     4201 cggcgaaatg ctcgaattca ccgcccagga aagcggcgag gtagtcaccg ggttggtgct
     4261 caatctggaa gaagacaacg tcggcgccgc gatcatgggc gactacctca agctcaaaga
     4321 gggcgacgag gtccgccgga cccgccggct gctcgaggtt ccctccggct cggcgatgct
     4381 gggccgtgtg gtggatgcct tggggcgtcc ggtggacggg agaggtgaga tcaaggccag
     4441 cgcctcgcgg ccggtcgaga tgatcgctcc tggcatcatc gtgcggcagc cggtgaagga
     4501 gccgctcc
//
LOCUS       DASRJQ010000367         3182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2653896, whole genome shotgun sequence.
ACCESSION   DASRJQ010000367 DASRJQ010000000
VERSION     DASRJQ010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2653896"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VFB89_08220"
     CDS             <1..622
                     /locus_tag="VFB89_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27328.1"
                     /translation="VRKPAADWIALTARDGRRGPQWPAVFGHDDGRWREMTVTVDGLW
                     RWAFRGGSSEQSYRAWVSASVSWLLGGVDTVRGVARPVRAVVPNGRPVVFEWLGPGAV
                     MPVRVDWNGGKAADTLHFDGAGRAAVWLSPGQYRYRLGPGGSGTVAVEEYSDELLPGP
                     VTISPHEARGSVARSTRSAREWPWLFALCVLGLAGEWLARRRLGLR"
     gene            716..1639
                     /gene="ftsY"
                     /locus_tag="VFB89_08225"
     CDS             716..1639
                     /gene="ftsY"
                     /locus_tag="VFB89_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HZM27329.1"
                     /translation="MARRLGDTKQSLWSRLKRLAFTDVNAIIRGLNADDLEQLERLLI
                     EADFGVPATVALTQGLEDEVRKGTLKTEADLKRALTERISGMLAASGNGGFLATSADA
                     GPTVILMVGVNGTGKTTTVAKLGRLLARQGRKVLLAAADTYRAGAVSQLQIWAERLGL
                     PCVAGAPGGDPAAVAFDAIEAARSRGADTVIVDTAGRLHTQEGLMDELRKVVRVIARR
                     VPGAPHETLLVLDGTVGQNAIQQGRLFSDAVSPTGIVVTKLDGSARGGAVVALTKELG
                     LPIRFVGLGETLDDLEPFDAERFAANLVGTS"
     gene            1664..>3182
                     /locus_tag="VFB89_08230"
     CDS             1664..>3182
                     /locus_tag="VFB89_08230"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HZM27330.1"
                     /translation="MSAPPIRLDTPVKFLKGIGERRAEALARLGIRTARDLLWHLPHR
                     YVDASSVTPVANARVGMEVACVGQVVSKGVLPTRKGLRIFHAVLRDSSGVLECAWPGQ
                     PFLDRTIEVGQTLLVSGPVRFYHGRQMAPREYLILSEADGETEGESDPLGAGKVLPVY
                     PATEGLSHKVIRGLIDRHLDGLIAASHDIIPERLRLAAGLPIMADALRMVHRPTSVAA
                     AEQGRRRLAFDELLDLQLMLVRARTLAKRARSGVAFQIKRELTTRLKQSLPWSLTEDQ
                     QRALKEITVDMTAPERMHRLLMGDVGTGKTVVALFAMLLAMENDFQAALMAPTELLAE
                     QHVATLSRLLEALSIKPELLVGRLSAAEKGAVRERLAAGQSRLVVGTHALLEDTTAFQ
                     RLGLVVIDEQHRFGVEQRAALIGKGAAPDVLLLSATPIPRSLALTLYGDLDVSTLRQR
                     PPGRGEVRTAVRTANQRERVLQFVEKECAEGRQAYVVLPVIEESERADLRAATTMA"
BASE COUNT          512 a         1018 c         1101 g          551 t
ORIGIN      
        1 ggtacgcaag ccggctgccg actggatcgc gctcaccgct cgtgacggac gccgcggtcc
       61 gcagtggccc gccgtcttcg ggcacgatga cgggcggtgg cgtgagatga cggtgacggt
      121 cgacggtctc tggcgctggg cattccgcgg cggttcgagc gagcagagtt accgtgcctg
      181 ggtgtccgcg agtgtgagct ggctgctggg tggagtagac accgttcggg gtgttgcccg
      241 cccggtgcgg gcagtcgtcc ccaacggccg gccggtcgtg ttcgagtggc ttggccccgg
      301 agcggtaatg ccggtacgcg tggactggaa cggtggaaaa gcagccgata ccctgcattt
      361 cgacggagcg ggccgagccg cggtgtggtt gtcgccgggc cagtatcgct atcgcctcgg
      421 ccccgggggc agcggcaccg tagccgtcga agagtactcc gacgaattgc tgcccggccc
      481 agtcaccatc tcgcctcacg aggcgcgggg tagtgtcgcc aggtcgactc gctccgctcg
      541 ggaatggccc tggctctttg cgctctgtgt gctcgggttg gcgggggaat ggttggcgcg
      601 acggcggctg ggacttaggt agactacgtg aacggcaaac ggtgaacggt aaacggggtg
      661 ttgccgaaac tgactcccgt ttaccgttca ccgttcaccg ttcaccgtgg ttccaatggc
      721 ccgccgtctc ggcgacacaa aacaatccct ctggtcccgg ctcaagcggc tggcgttcac
      781 cgacgtcaac gccatcatcc ggggtctcaa tgccgacgat ctggagcagc tcgagaggct
      841 cctcatcgaa gccgatttcg gcgttccggc tacggtggca ttgacccagg gtctcgagga
      901 cgaagtacga aaaggcaccc tcaagacgga ggccgatctc aagcgagccc tgacggaacg
      961 cattagcggc atgctcgccg cctcgggcaa cggtggtttc ctggcgacat cggcggatgc
     1021 tggtcccact gtgattctca tggtcggcgt gaacggaacc ggcaagacca ccaccgttgc
     1081 caagctgggc aggctgcttg cccgccaggg aagaaaggtc ctgctggcag cggcagacac
     1141 atatcgggcc ggagccgttt ctcagctcca gatctgggcg gaacgtctcg gcttgccttg
     1201 cgtcgccgga gcgccgggcg gcgatccggc agcggtcgca ttcgacgcca tcgaggctgc
     1261 ccgctcgcgc ggagccgaca cggtaatcgt ggatacggcg ggacggttgc acacccagga
     1321 aggtctcatg gacgagctcc gcaaggtcgt gcgggtgatc gcccgacggg tacccggcgc
     1381 gcctcacgaa acccttctcg tactcgatgg aaccgtcggc cagaacgcga ttcaacaggg
     1441 ccggcttttc agcgacgcgg tctcacccac cggcatcgtc gtcaccaagc tcgacggcag
     1501 cgcgcgtggg ggggccgtcg tggctctgac aaaggagctg ggccttccca ttcggttcgt
     1561 cggcctgggc gagactctcg acgacctgga gccgttcgac gccgaacgct ttgccgccaa
     1621 cctggtcggc acctcgtagc cagcaatagc aaccatgcct cccgtgtccg ccccacccat
     1681 tcgcctcgac acgccggtca agtttctcaa gggcattgga gagaggcggg ctgaggcgct
     1741 ggcccggctt gggatccgga cggcgcgcga cctgctctgg catctgccgc accgttacgt
     1801 cgacgcctca tcagtcaccc cggttgccaa cgcgcgagtc ggaatggaag tcgcttgcgt
     1861 ggggcaggtc gtcagcaagg gcgtgctgcc cacccggaag ggcctccgaa tttttcatgc
     1921 ggtcttgcgg gattcgagcg gcgtgctcga atgcgcctgg ccgggtcagc cttttctcga
     1981 tcgcaccatc gaggtagggc agacgctgct cgtgtcgggg ccggtccggt tctatcacgg
     2041 gcggcagatg gcaccccgcg agtacctcat tctgagcgag gcggacggtg agacggaagg
     2101 cgagagcgac ccgctaggcg cgggcaaggt gctgccggtc tatccggcta ccgaaggact
     2161 gagccacaag gtcatccgtg gcttgatcga tcggcacctg gacgggttga tcgcggcgtc
     2221 gcatgacatc atccctgagc ggctccggct tgcggcgggc ctgcccatca tggccgacgc
     2281 gctgcggatg gtccatcgcc ctacgagtgt cgcagcggcc gagcagggcc gtcggcgcct
     2341 ggccttcgac gagttgctgg acttgcagct gatgctggtg cgagctcgaa cgctggccaa
     2401 gcgggcgcgg tcgggtgtcg cctttcagat aaaacgggag ctgaccaccc gcctcaagca
     2461 aagccttccc tggtccctca ccgaggacca gcagcgcgcc ctgaaggaga tcacggtgga
     2521 catgaccgct cccgagcgga tgcatcggct gctcatgggc gatgtgggca cgggtaagac
     2581 ggtagtggcc ctgtttgcca tgctgctcgc gatggagaac gattttcagg ccgcgctgat
     2641 ggccccgacc gagctcctgg ccgagcagca cgtcgcgacg ctctcccgcc tgctcgaggc
     2701 gctctcgatc aaacccgagc tcctggtcgg ccggctcagc gcggccgaga agggcgccgt
     2761 gcgggagcgc ctcgcggcgg gacagtctcg cctggtcgtc gggacccatg ctctactgga
     2821 ggacaccacg gcgttccagc gtctcggcct ggtcgtgatc gacgagcagc accgtttcgg
     2881 tgtcgagcaa cgcgccgcgt tgatcgggaa aggcgcggcg ccagatgtgc tgctgttgtc
     2941 cgcgaccccg attccgcggt cgctggctct gacactgtac ggggacctcg acgtatccac
     3001 gctgcgccag cggcctccgg gccgtggcga ggtgcggacc gccgtgcgga cggccaacca
     3061 gcgcgaacgg gtgctccagt tcgtcgagaa ggagtgcgct gaagggcgcc aggcgtacgt
     3121 ggtgcttccc gttatcgagg aatccgaacg ggccgatctc cgggctgcaa ccacaatggc
     3181 cg
//
LOCUS       DASRJQ010000368         3506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2843166, whole genome shotgun sequence.
ACCESSION   DASRJQ010000368 DASRJQ010000000
VERSION     DASRJQ010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2843166"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VFB89_08235"
     CDS             complement(<1..233)
                     /locus_tag="VFB89_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27331.1"
                     /translation="MKRSIVAVVALLTHGPGSASEPMRMSCRVRVPAAYQRDPPGWQS
                     PTGESEAERYSRAYEAFWWNCIMVRADSLDARCR"
     gene            complement(859..1368)
                     /locus_tag="VFB89_08240"
     CDS             complement(859..1368)
                     /locus_tag="VFB89_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27332.1"
                     /translation="MKRCSLAALSVLLVLASPRGASSVELKRPSLAGLSRFEAVIVTV
                     SDDTVDEEALRRTVEDRLAKAKIAVDGAPGPHLYVTVSAERNKSDVGTCEFGNFKSVI
                     AVLESVRLDRAPDLGSFSVTTWRKEGQVRRFSLKPPRLGIMDNVNDGLTLMIQTIAAD
                     TKRTEQERQ"
     gene            complement(1488..3002)
                     /locus_tag="VFB89_08245"
     CDS             complement(1488..3002)
                     /locus_tag="VFB89_08245"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HZM27333.1"
                     /translation="MIFPGGQKQAGILFLSVCSTVPLLAGQNRTPPADSRPNIVLILA
                     DNVGYGELGVYGGGILRGAPTPRIDRLASEGLRLLNFNVEAQCTPSRSALMTGRFSIR
                     SGTYQVPRGGAPEGLTQWEITIAELLSAQGYATGIWGKWHLGSTEERFPTHQGFDEWY
                     GIPRTYNEALWPSLNETNSPWPSVGSRQGWNAELVPPEPVYEARKGENARQVAQLDLE
                     RHRRMDEEITSRAVAFIKRQAGSGKPFFAYVSLRLLHMPTTPHPEFTGRTGNGDWADC
                     LSELDHRTGQILDAIREAEIEGNTLVIFASDNGPESTHPWQGDSGPWRGTYFTAMEAS
                     LRAPFIIRWPTRVPAGAISNEIVHITDLYTTLARVGGAEVPKDRPIDGVDQLDFFLGK
                     QASSNREGFPAYVADRLSAVKWRNWKMHLIRQDRRDDPPQKLPLPRIINLLTDLKEER
                     DVLTPSGWVTYPMMKILRDFEASLETYPPIKVGTPDPYLPPASTGRRDGSGAKP"
BASE COUNT          610 a         1105 c         1132 g          659 t
ORIGIN      
        1 cggcatcgcg cgtctagact gtccgctctc accatgatgc agttccacca gaaggcttcg
       61 tacgctcgac tgtatctctc ggcctcggac tcccctgttg gtgactgcca acccggagga
      121 tcacgctggt aggcggcggg cacgcgtacc cggcacgaca ttctcattgg ctctgacgcg
      181 ctgcccgggc cgtgtgtgag gagggccacc acggcgacga tcgatcgctt cacgtgcctc
      241 cctctgaagg caccgaacac cgagataagc agcgaagacc gcgccattct agccaccgcg
      301 ggcttcgtct gcttcatctc tttgttgggc cgccggcgct gcgctcatca cagagcagag
      361 ttacccagcc agaccttgta cgtccgtccg cctgagccgg ccgaaagacc cgcatcgcga
      421 ggcgctcgag gcaccgagcg tcaatctgag gaacggaccg gaccacgcgt gcccgaatga
      481 ctcggcccga tgcatcgacc tcgccttcca tctcgacgcc aggaatgctg gcagcacggt
      541 taacgcacgc ttcctctgac gcattcggac taagcaagcg tggcgtggtg attctgtctc
      601 ccggggttct gtcggaagaa cacagttgag cgaggtggag actagcggtc ggcgtgaccg
      661 gcggagaggc cgccgaaacc ggtgtgcgtg atgcacaggc cgtgagaagt acgaaaccaa
      721 acgcgacggc ggccctcact ggcagccctg tctcaaggtc ggcccaacac ccgcatcagc
      781 tgcaagggcc gcggcgagac cgccttgccg cgaccggcgc ggaccttgtc agctgcaagg
      841 ttgttatgcg gctcattgct attgtcgctc ttgctcggtc ctcttcgtgt cggcggcgat
      901 tgtctggatc atcagggtga gcccgtcgtt cacgttgtcc atgatgccta ggcgcggggg
      961 cttgagggag aagcgtcgca cttgaccctc cttccgccac gtggtgacgc tgaaggaccc
     1021 aagatcaggc gctcgatcta gcctgaccga ttcgaggacg gcgatgacgc tcttgaaatt
     1081 gccgaactcg cacgtgccaa cgtcagactt gttgcgctcg gcagatacgg tgacgtagag
     1141 gtggggccca ggagctccat cgaccgcgat cttggctttg gccagacggt cctcaacggt
     1201 gcggcggagc gcctcctcgt cgacggtgtc atccgacacg gtgacgatga cagcttcgaa
     1261 gcgggatagg ccggccaacg atggtctctt caactcgacg gacgatgcac ctcggggtga
     1321 agccaacacc aggagcacac tcagggcggc gaggctgcat ctcttcatca ggttcctcca
     1381 taggtgcgca tcgagtccgc ataacaccgg gttaagcggc gaagccccat cgcttgcgcc
     1441 ggcttcgtcc gcttcaaccc atcgttgggc agccactcca ctctccatca aggcttcgcg
     1501 ccgctgccgt cgcggcggcc ggtcgacgct ggcggtaagt agggatccgg cgtgccgacc
     1561 ttgatcggag ggtatgtctc cagactggcc tcgaagtcgc ggaggatctt catcatcggg
     1621 taggtgaccc agccgcttgg tgtcaggacg tcgcgctctt ccttcagatc ggtcaggagg
     1681 ttgatgatcc tgggcagcgg aagcttctgc ggggggtcgt ccctgcgatc ctgccggatc
     1741 aggtgcattt tccagttccg ccatttgacc gcggacagcc ggtcggccac gtaggcgggg
     1801 aacccttcgc ggttggagct cgcctgcttg cccaggaaaa agtccaactg atcgacgccg
     1861 tcgatcggcc gatccttcgg gacctcggcg ccgcccacgc gggccagcgt cgtgtacaga
     1921 tctgtgatgt gcactatctc gttgctgatg gcccccgcgg gcactcgggt cggccagcga
     1981 atgatgaaag gcgcgcgcag cgaggcttcc atcgccgtga agtaagtgcc ccgccacggc
     2041 ccgctgtcgc cctgccaggg gtgagtggac tccggaccgt tgtcgctggc aaagatgacg
     2101 agggtgttgc cttcgatctc cgcttcccga atggcgtcca ggatctggcc cgtgcggtga
     2161 tctagctcgc tcaggcaatc ggcccagtcc ccattgccgg tcctaccggt gaactctggg
     2221 tgcggcgtgg tgggcatgtg aagcagcctc agcgacacat aggcgaagaa gggcttcccg
     2281 gagcccgcct gtcgcttgat gaatgcgaca gcacgggatg tgatctcctc gtccatccgc
     2341 cggtgtcgct ccaggtcgag ttgcgcgacc tgccgcgcgt tttctccctt gcgtgcctcg
     2401 tagacaggct caggcggaac gagctcggcg ttccagccct gacggctgcc caccgatggc
     2461 caagggctgt tggtctcgtt caaggacggc catagggcct cgttgtaggt gcgtggaatc
     2521 ccgtaccact cgtcgaagcc ctgatgggtc gggaagcgct cctcggtgct gccgagatgc
     2581 cacttgcccc aaatgccggt ggcatagccc tgcgcggaca agagctctgc gatggtgatc
     2641 tcccactggg tcagcccttc cggggctccc cctctcggga cctggtaggt cccggagcga
     2701 atcgagaagc ggcccgtcat gagcgcggaa cgggaggggg tgcactgcgc ctcgacgttg
     2761 aagttgagca ggcgcaggcc ctcgctggcg agccggtcga tacggggcgt gggggccccg
     2821 cgcaggatcc cgccgccgta gacgcctaac tccccataac ccacattgtc cgcgaggatg
     2881 agaacgatgt tgggcctcga gtccgccggc ggcgtccggt tctgccctgc gagcaagggg
     2941 actgtgctgc aaacgctcag aaacaggatt ccagcctgct tttgtccacc cggaaaaatc
     3001 atggcctccc cttgtgatgg gtgaagcgac tcgttgactg aatactgctc cgatttgagc
     3061 gactgggcgc agggccggcg tcgcagacgc caagactcag gagcattcga gggttatcca
     3121 cgtactcatg agtcggggca gttctttgcc ggtctgccca acaccggata cacagacaag
     3181 atcgtcccat tattcttgca agccgagggg caactgctgg gccggctacg catatggggc
     3241 gagttcggcc ggattatcga gacaagctaa tccgttaacg ctgcgggcgg tggtgagatt
     3301 tatccgcccc ggggcggata cggaaatcgt aagcgggagt tccgtcaccg tgatgcgttg
     3361 acgttctcgt ccagccaggc caggtagggc gcgtgcccgc cggccaccgg caccgcgatc
     3421 acctccggaa ggtcgtaggg gtggagcgcg accaccgcgt cgcgcacggc ggagaagcgg
     3481 tccgcgcgcg tcttgatcag gagcag
//
LOCUS       DASRJQ010000369         2101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_336957, whole genome shotgun sequence.
ACCESSION   DASRJQ010000369 DASRJQ010000000
VERSION     DASRJQ010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336957"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VFB89_08250"
     CDS             complement(<1..287)
                     /locus_tag="VFB89_08250"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM27334.1"
                     /translation="MTEPPSALTAALVDRYRIERELGSGGMATVFLAQDLKHRRRVAI
                     KVFAPEVAAAFGVGRFLREIEIAAHLQHPHILPLFDSGEAGGLLYYVMPFV"
     gene            complement(750..1934)
                     /gene="wecB"
                     /locus_tag="VFB89_08255"
     CDS             complement(750..1934)
                     /gene="wecB"
                     /locus_tag="VFB89_08255"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HZM27335.1"
                     /translation="MSPSGRQSHRIAVVLGTRPEAIKMAPVIQELRRRPDQFETVVIT
                     TSQQREMLAQAMHAFSLCSDIDLGLSHANQSLADFTARALIALTGAFRQVKPDLLLVQ
                     GDTSTVIAAALAAFYHGVPIGQVEAGLRSGDARRPFPEEVNRRMASCVTDIHFAPTER
                     ARSNLQAERVPDEHIYVTGNTIVDALASIRHDGVFDDPALNALGWESKQIILATVHRR
                     ENLGQNLRNVCAAFRHLVETHAKTEIVIPVHLNPYVREIIFEELHAAPGVTLLEPLEY
                     PDLIEVMRRSVLVLTDSGGIQEEAPAMRKPVLILRTVTERPEVVESGYGKLVGTDRDL
                     IVEEASHILSDPVAYRAMISGGNPFGDGHAATRIVDILTERFRTGRLFSAPVVDAPVP
                     QH"
BASE COUNT          387 a          582 c          707 g          425 t
ORIGIN      
        1 tcgacgaacg gcatgacgta atagaggagc cctccggctt cgccggagtc gaacagcggg
       61 agaatatggg ggtgctggag gtgggcggcg atctcgatct cgcgcaggaa ccgacccacc
      121 ccgaatgccg ccgcgacttc cggggcaaaa accttgatgg ctaccctgcg ccggtgcttg
      181 aggtcctggg ctaggaagac cgtggccatc cctccgctac caagctcgcg ctcgatccga
      241 taccggtcca caagggccgc ggtgagcgca ctggggggtt cggtcatgaa ggcgggcggc
      301 ggcctggcga gggtgaacct gtaacttgta gccgtatagg cagatacgcc agtcgtaaaa
      361 ggcagcctgt ggatctttac ttgagctcgg gggcgaaact cgaagctgct gctcaatgtg
      421 ccgcccatgc gggaggaact gtgtggattc ggacggcaaa cccgggaaag ccttcggcgg
      481 gactgttctg caggatggtt ctgcgtgcaa ataggtttga tttggagtcc tgttagtcta
      541 aataaccaac tattgtcgag ctatttgctg ccctagttga ttggaggttg agccgtccat
      601 tttggtgggg tcagatcgct gttcaacgtg tcgtggaacc acagatttca ccgataaaaa
      661 tagatttctg cagtcacaat attgattagg tcaatgtatt ctctagttat ttatgttgtt
      721 tagctgacaa ctgttcatgg cttaccaatt cagtgctgcg gaacgggtgc gtccacgacg
      781 ggtgccgaga agaggcggcc ggtccggaat cgctcggtga ggatatccac gattcgggtc
      841 gcggcgtggc cgtcgccgaa gggattgccc ccgctgatca tggcgcggta tgcgacggga
      901 tccgagagga tgtgggacgc ctcctcgacg atcaggtcgc ggtcggtgcc gaccagcttg
      961 ccgtatcccg attccaccac ttcggggcgc tcggtgacgg tgcgcagaat gagcaccggc
     1021 ttgcgcatgg ccggcgcttc ttcctggatt cctccggagt cggtgagcac gagcacgctc
     1081 cgccgcatga cctcgatcag gtcgggatac tccagcggct cgagcagggt aacgcccggc
     1141 gccgcatgca gctcctcgaa gatgatctcc cgcacgtagg gattgaggtg caccgggatc
     1201 acgatctcgg tcttggcatg ggtttcgacc agatgccgga acgcggcgca gacattccgc
     1261 agattctgtc ccaggttttc ccggcggtgc accgtcgcca gaatgatctg cttggactcc
     1321 catcccagcg cgttgagggc cgggtcatcg aacactccgt cgtggcggat cgaggcgagc
     1381 gcatccacga tggtgttgcc ggtgacgtag atgtgttcgt cggggacccg ttccgcctgc
     1441 aggttggacc gggcccgctc ggtgggcgca aaatgaatat cggtgacgca gctcgccatc
     1501 cggcgattga cttcctcggg aaacggtcgg cgggcatcgc cggagcggag tccggcctcg
     1561 acctgtccga tcgggacgcc gtggtagaag gcggcaaggg ccgcggcgat gacggtgctg
     1621 gtgtcgccct gcacgaggag gagatctggc ttcacctgcc gaaacgctcc ggtcagcgcg
     1681 atcagcgcgc gggccgtaaa gtccgccagc gactgattgg catggctgag gccgaggtcg
     1741 atatcggagc agagcgagaa ggcgtgcatc gcctgggcca gcatttcccg ctgctgcgac
     1801 gtggtgatga cgacggtctc gaactggtcg ggacggcgcc ggagctcctg gatgacaggc
     1861 gccattttga tcgcctcggg gcgggtgccc aggacgactg cgatgcggtg gctctgcctg
     1921 cccgagggag acatgagcct tctaaacgca atttagaggc cgggtggact gcccatgtaa
     1981 accattgcag ggaaggaagt tttcttgcat gccccggctt caggcctgat gggtaaagcg
     2041 caatctccgg ctaccggtac ttcgcggtat atcgtgcgat cgagtcgctg atggtgcggt
     2101 a
//
LOCUS       DASRJQ010000370         4192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_264084, whole genome shotgun sequence.
ACCESSION   DASRJQ010000370 DASRJQ010000000
VERSION     DASRJQ010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264084"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1751)
                     /locus_tag="VFB89_08260"
     CDS             complement(<1..1751)
                     /locus_tag="VFB89_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HZM27336.1"
                     /translation="MTHLPSSVEPIDRAARDARVAQLRPLLEQRILVLDGAMGTMIQA
                     HKLAERDYRGERFADWPRDLKGNNDLLSITQPIIIAGIHRAYLDAGADIIETNTFNST
                     AASMADYGMEELVYELNLQGAHLARAAADAAEAREPGQPRFVAGAMGPTNRTASISPK
                     VHDPSFRNVSFDELAATYAEAARGLLDGGADLLLVETIFDTLNAKAAVFGIQSVFEER
                     GDRVPLMISGTITDQSGRTLSGQTPEAFWYSLMHAEPLIVGFNCALGGKALRPHVQEL
                     ARVAPVYVSAYPNAGLPNEFGNYDETAQITASVLREFAEHGLVNLVGGCCGTTPAHIR
                     AISEAVRGLPPRRPPELPHRCRLSGLEPLVIGPQTNFVNVGERTNVTGSRRFAKLILE
                     GQYEAGLEVARQQVENGAQMLDVNMDEAMLDSAAAMQTFLRLVASEPSISRVPIMIDS
                     SKWSVIEAGLKCVQGKGVVNSISLKEGEDAFIHHARLVRRYGAAVIVMAFDERGQADT
                     VERKVQICTRSYRILTEQVGFPPEDVIFDPNVFAIATGIEEHADYAVGYIEATRRIKA
                     ELPHALVSGGVSNVSFS"
     regulatory      complement(1787..1861)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            complement(1931..2257)
                     /locus_tag="VFB89_08265"
     CDS             complement(1931..2257)
                     /locus_tag="VFB89_08265"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HZM27337.1"
                     /translation="MDRPTGARARATLTPLSATPDGVRLRIRVQARASRTEVVGVHGD
                     EIRVRIAAAPVDGAANETLIRFLAKRLGVGRSAVAIVGGARAPSKAVQVTGVTWQRAA
                     SRLGII"
     gene            complement(2218..2784)
                     /locus_tag="VFB89_08270"
     CDS             complement(2218..2784)
                     /locus_tag="VFB89_08270"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HZM27338.1"
                     /translation="MLDTIARGVVVVVFLYAVVVATTQWAIRRRHLTPFGAWPRFVRR
                     ISEPVLLPLERRIIRAGGSPTDAPLWLLGIAIIGGLLLITLVRWLIGAVYEFGALSQA
                     PPRIWLIETVSWVFRILMVALLVRVIASWFGASPYAKWMRPVVLLTDWLLEPLRRVLP
                     PFGPLDLSPMVAYFLLWIAQQALVRALL"
     gene            complement(2793..3239)
                     /locus_tag="VFB89_08275"
     CDS             complement(2793..3239)
                     /locus_tag="VFB89_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27339.1"
                     /translation="MSWRAALAILLVLAGVAGCSESSPKPARRTSVLDTLPPERRDAA
                     LLGREVFRLIDKAADYRGSHLGHPPVELRVMGVDSLAPLFVRRIRVVNDSSLVTVAFR
                     QPAGRTVASCEGNPQILEEASLSGGTFTVVCTTREGTSAKYEVPAP"
     gene            complement(3236..>4192)
                     /locus_tag="VFB89_08280"
     CDS             complement(3236..>4192)
                     /locus_tag="VFB89_08280"
                     /EC_number="1.14.19.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045300 - acyl-[acyl-carrier-protein]
                     desaturase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HZM27340.1"
                     /translation="AREMFSKLEVLRDLEGKVRELMESHERKRELWYPAELIGPKEGE
                     CPETHYKQLRKRAEGIPDPARAALALNLLTEEGLPHFHRLLAVYLGDDSFWRLWNNLW
                     TAEEDRHGQVIHDYAREARLLHQRRLEEMQFEYLKAGFHPEWDRDPYRVFAYTTVQER
                     ATQHSHAETGRLVGEYEPILGEILSNVAKEEARHYTFYRTVFEEILKRDPDQALHSAS
                     FILPSIEMPGYSMPGFRDLADVIRRAGIYGPRDYLRIVQEQIRYWKIESIGGLSELGR
                     KAQEKIMSIPDRLRRVAELIETRSRAKTFSFEVVFNREFAME"
BASE COUNT          767 a         1389 c         1315 g          721 t
ORIGIN      
        1 aacgagaacg agacgttgct gactccgccg ctcaccaggg cgtgcggcag ctccgccttg
       61 atccggcgag ttgcttcgat atagccgacc gcgtagtcgg cgtgctcctc gataccggtt
      121 gcgattgcga aaacgttggg atcgaaaatg acgtcttcgg gcgggaagcc gacctgctcc
      181 gtgagaatcc ggtaggaccg cgtacagatc tgcaccttcc gctcgaccgt gtccgcctga
      241 ccgcgttcat cgaacgccat cacgatcacg gcggcgccgt accgccgaac cagccgggca
      301 tgatggatga atgcgtcctc cccctccttc agcgagatgg agttgacgac gcccttcccc
      361 tggacgcact tgagtcccgc ctcgatgacc gaccacttcg atgagtcgat catgatcggc
      421 acccggctga tgctgggctc cgacgcgacc aggcgcagga aggtctgcat ggccgccgcc
      481 gagtcgagca tggcttcgtc catgttgacg tcgagcatct gggcgccgtt ctcgacctgc
      541 tgccgtgcca cctccagtcc cgcctcgtac tgaccctcga ggatgagctt cgcgaaacgg
      601 cgtgaccccg tcacattggt ccgctcgccc acattgacga agttggtttg agggccgatc
      661 accagcggct ccaggccgct taagcggcag cggtggggca gctccggcgg tcggcggggc
      721 ggcaggccgc gcaccgcctc ggaaatggcg cgtatgtgcg ccggtgtggt cccgcagcaa
      781 ccacccacca ggttcacgag cccatgctcg gcgaattcgc gaagtaccga cgcagtgatc
      841 tgggctgttt cgtcgtagtt gccgaactca ttgggcaggc cggcattggg ataggcgctc
      901 acgtacacgg gagccaccct cgccagctcc tgcacatgcg gccgaagcgc cttaccgccc
      961 agcgcgcaat taaatccgac gatcagcggc tcggcgtgca tcagtgaata ccagaacgct
     1021 tcgggagtct ggccggacaa cgtgcgcccg ctctggtcgg tgatcgtgcc cgagatcatg
     1081 agcggcaccc ggtcgccccg ttcctcgaat acgctctgga ttccgaacac cgcggccttg
     1141 gcgttgagcg tgtcgaagat cgtttcgaca aggaggaggt ccgccccgcc gtccagcagc
     1201 cctcgcgcgg cctcggcgta cgtcgcggcc agctcgtcga agctcacatt ccggaagctg
     1261 ggatcgtgaa ccttgggcga gatggaggcg gttcggttgg tcgggcccat cgctcccgcc
     1321 acgaaccgcg gctgccccgg ctcgcgagcc tccgctgcgt ccgccgcggc acgggctagg
     1381 tgggcgccct gcaggttcaa ctcgtagacc aactcctcca ttccataatc cgccatgctg
     1441 gcggcggtgg agttgaatgt gttggtctcg atgatgtccg cacccgcgtc aagatatgcg
     1501 cggtggatcc cggcgatgat gatgggctgc gtgattgaga ggaggtcgtt gttgcccttg
     1561 aggtcccgcg gccaatcggc aaaccgctcg ccgcggtagt cccgctccgc gagcttgtgc
     1621 gcctggatca tggttcccat tgccccgtcc agcaccagga tccgctgctc gagcagcggt
     1681 cgcagttggg cgacccgcgc gtcgcgcgcg gcccgatcga tcggctccac agatgagggc
     1741 aagtgggtca tggccgctcc ctgcaaacaa aaaagccggc gccaaaacgg ccccggggta
     1801 ggcgttttag cggattgttt aacgtggccg caagctgccc caaatcgcca cggaacactc
     1861 tggttgactg ctctcaagct aattcggtgt ccgcccctat caagcccggg acttcccgga
     1921 ccccacctga ttagatgatt ccgagccgac tggcggcccg ctgccacgtg actccagtca
     1981 cttgaacggc tttgctgggg gcgcgggctc cgcctacgat cgccacggcc gaccggccca
     2041 ccccgagccg cttggcgaga aaccggatca acgtttcgtt ggcggcgccg tcgaccggag
     2101 ctgccgcaat gcgaacccgg atctcatcac cgtggacccc aaccacttca gtgcgtgagg
     2161 ctcgggcctg cacccggatc cggagccgca cgccatcggg tgtcgccgag agcggggtca
     2221 gagtagcgcg cgcacgagcg cctgttgggc gatccacagg aggaagtagg cgaccatggg
     2281 gctcaggtcc agcggaccga acggcgggag cacgcggcga agtggctcca acagccagtc
     2341 ggtcagcaag acaacaggtc gcatccactt ggcgtacggg gaggctccaa accacgaggc
     2401 gatgacgcgc accagcagcg cgaccatcag gatcctgaat acccacgaca cggtttcgat
     2461 gagccagatc cgaggcggtg cctgcgaaag agccccgaac tcgtacaccg ccccgatgag
     2521 ccacctgacc agcgtaatca gcagcaggcc gccgatgatg gcgatgccca gcagccagag
     2581 cggggcgtca gtggggcttc caccggcccg gatgatccgg cgttcgagcg gcagcagcac
     2641 cggctcgctg atccgacgga cgaaccgagg ccacgctccg aacggcgtca ggtggcggcg
     2701 ccggatggcc cattgagtgg tggcaaccac gactgcatac aggaacacca cgaccacgac
     2761 gccgcgagcg attgtgtcga gcatgaccaa catcacggtg cgggcacctc gtacttcgcc
     2821 gaggtaccct cgcgggtggt gcataccacc gtgaaggtac cgccgctcaa gcttgcctcc
     2881 tccaggatct gcggattgcc ctcgcaggaa gcgacggttc gtccagccgg ctgccggaat
     2941 gcgaccgtaa cgagggagga atcattgacc acgcgaattc gccggacgaa cagtggcgcc
     3001 aggctgtcga cacccatcac gcgcagctcg acaggaggat gacccaggtg ggaaccgcgg
     3061 taatctgcgg ccttgtcgat caggcggaac acctcgcgac cgaggagggc cgcatcgcga
     3121 cgttccggag ggagcgtgtc gagaaccgac gttcgacggg ccggtttcgg ggaggactcg
     3181 ctgcagcccg cgacccccgc cagcaccagc aggatggcaa gcgcagccct ccagctcatt
     3241 ccatggcgaa ttcgcggttg aacacaacct cgaagctgaa cgtcttggct cgactgcgcg
     3301 tctcgatcag ctccgcaacc cggcgcaacc gatcgggaat gctcatgatc ttttcctggg
     3361 ccttccgccc cagctccgac aatccgccga tgctctcgat cttccagtaa cggatctgtt
     3421 cctgcacgat ccgcaagtag tcgcggggtc cgtaaatacc cgcccggcga atcacgtcgg
     3481 ccaggtcccg aaatccgggc atggagtagc cgggcatctc gatactgggg aggatgaacg
     3541 acgcggagtg gagagcctgg tccgggtcgc gcttcaggat ctcttcgaag acggtccgat
     3601 agaaggtgta gtgccgagcc tcttccttgg cgacgttgct gagaatctca cccagaatcg
     3661 gctcgtactc gccaacaagc cgtccggtct cggcatggga gtgctgcgtc gctcgttcct
     3721 gaaccgtggt gtaggcgaag acgcggtacg ggtcccggtc ccactcggga tggaagcctg
     3781 ccttcagata ctcgaattgc atctcctcga gccgccgctg atggagcagc cgagcctctc
     3841 gggcgtagtc gtggattacc tgcccgtgcc gatcctcctc ggcggtccaa agattgttcc
     3901 agagccgcca gaagctgtca tcacccagat ataccgcaag caaccgatga aagtgcggaa
     3961 gcccttcttc ggtgaggagg ttgagcgcga gtgccgcgcg ggccgggtct ggaatcccct
     4021 cagcccgttt acggagctgc ttgtaatggg tctccgggca ctccccctcc ttagggccga
     4081 tcagctccgc cggataccac aactcgcgct tccgctcgtg gctctccatc aactcgcgga
     4141 ccttcccttc cagatcccgg agcacttcga gcttggaaaa catctcgcga gc
//
LOCUS       DASRJQ010000371         6377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_832017, whole genome shotgun sequence.
ACCESSION   DASRJQ010000371 DASRJQ010000000
VERSION     DASRJQ010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6377
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832017"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VFB89_08285"
     CDS             <1..549
                     /locus_tag="VFB89_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HZM27341.1"
                     /translation="AEEAPAFRSLNLLTSKPVLYAANVSESEITEGNRFVDALVSAVQ
                     RDGEQAEVVTFSAKVEAELAELAPEDRTEFLASLGLKESGLDRLAHAAYHLLGLQSYF
                     TAGEKEVRAWTIHRGDRAPEAAGVIHSDFEKGFIRAETVGYDDFVRVGGWKPAREQGL
                     ARAEGKEYVVQDGDVMLFRFSP"
     gene            631..972
                     /gene="rpsF"
                     /locus_tag="VFB89_08290"
     CDS             631..972
                     /gene="rpsF"
                     /locus_tag="VFB89_08290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HZM27342.1"
                     /translation="MTRQYEVVYIFDSALEEAAINERLARYHTLIQQPGVETPQVSHW
                     GKRTLSYPIKKHETGYYVVAKFDTDGAALPEFERAVKLDESVLRFLVVVNEGAQPVPV
                     TTAKGDEEDDE"
     gene            969..1190
                     /gene="rpsR"
                     /locus_tag="VFB89_08295"
     CDS             969..1190
                     /gene="rpsR"
                     /locus_tag="VFB89_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HZM27343.1"
                     /translation="MRRPQKTCAICESGVRIVDYKDERTLQRFLTERGKILPSRLSGT
                     CARHQRQLSTAIKRARQLALLPYIKGFGG"
     gene            1205..2140
                     /locus_tag="VFB89_08300"
     CDS             1205..2140
                     /locus_tag="VFB89_08300"
                     /inference="COORDINATES: protein motif:HMM:NF021506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HZM27344.1"
                     /translation="MSGLPPGRRARPGVLALALAYLLLAPPGFLLGPLAGLLALSRPT
                     TFREWAWLGLATGWMCVWLVPAGNLMDQLLRATAVLITGGFLAITLVGRAPVFRRGLA
                     ALGFATAALVLWASAFHLAWSDLKAVIGRDAARSLSALASQAATAGSDEAAELLRQMA
                     TSAPSMAMLTPALVFLSGLLGTLLAWNLYQGIAVRAMGDPPAPFPSFRFSDQMVWLPV
                     AGLALVLFPPVPALGEVGANLLLVAVALYAARGLAVVGAGAGRVPRLTTTFVALIAFF
                     LLPFVVGGLTLLGLADTWLDFRGRPASSGTGGFDR"
     gene            2137..2583
                     /gene="rplI"
                     /locus_tag="VFB89_08305"
     CDS             2137..2583
                     /gene="rplI"
                     /locus_tag="VFB89_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HZM27345.1"
                     /translation="MIEVILREDVKSLGRAGELVRVRPGYARNFLLPKGLAFEATEGN
                     KKRIAAESKARSGRVEAERTEAQAFANRLAEITLTVAGKAGEEGKLFGSITAHDIAEG
                     LAKQGVEVDRRRIELGQPIKTLGFHTVAVRLHPDVHAEVRVQVVGE"
     gene            2543..4444
                     /locus_tag="VFB89_08310"
     CDS             2543..4444
                     /locus_tag="VFB89_08310"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HZM27346.1"
                     /translation="MSTQRFGSKSSASNLAMTVGIAYLDGPRLARSLFAAADWVAAGR
                     DEINRINVFPVPDGDTGTNFSLTLRAVADALRALGDASLPETANTAARAAVLGARGNS
                     GMMLAHFLMGFAEAIGDRHHATTTDIAAALRRGADRLYESLDDPREGTILTVAREAAA
                     AAERAAADCEDISEFMSRLLAEAEQALARTPELMTVLKEAGVVDAGGKGFVRMIEGVV
                     RFIEGDPILPAAAEVGPPITIPAALIGVAAERDFQYCTEVLVRGAQLPPANEVRAAMH
                     NFGGSVQVAVMSDVLKVHVHTDTPEAVFSYAARWGRIETTKAEDMRAQHRKLSHDTRR
                     PVAVVVDSSADLADAILDRHHIAIVPLQVVFGNETFRDRVELRPDEFYRRLRIAKDLP
                     TTSQPAPAEFVRVFRDALQEADEVVAVVLAGALSGTHQAGVAAVKAAGLSNVIVVDSA
                     SVSLGAGMLALRAAELAESGWRSPDIATELERLKGQSGALITVDQYDNLLRSGRVTRG
                     KAWVASLLDVKPILSLDSAGRVVPVDRVRGRDNVAPRVLSLLERRLTPRPKAVRFGIA
                     HADAPDVAERVRTALVAAYRPRDCFVSMATGVLGTHVGEGAWGVFYQIEDGTPTLLER
                     GRVSGDWPA"
     gene            4441..4791
                     /gene="rsfS"
                     /locus_tag="VFB89_08315"
     CDS             4441..4791
                     /gene="rsfS"
                     /locus_tag="VFB89_08315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HZM27347.1"
                     /translation="MTPPFPEALGLALEAIDDLKGVQPVVLDLRGLSNATDFFVIASG
                     TSDAHVRGIAENVMERLGRRGHRTHHVEGLGGRWVLLDFVDFVIHLFHPETRAFYQLE
                     RLWGDAPELLIRSS"
     gene            4804..>6377
                     /locus_tag="VFB89_08320"
     CDS             4804..>6377
                     /locus_tag="VFB89_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27348.1"
                     /translation="MVRRCTVALALLLGTPALLEAQYFGQNKVQYESFDWKIIQTEHF
                     DVHFYPAEREAAMDAARIAERSYARLSKVLGHRFRERKVIILYASHSDFSQTNTTEGD
                     VGEGTGGFTDFFKQRNIMPLTGAYADIDHVLTHEMTHQFQLDVFSSGRGGSALQGVLQ
                     LQPPLWFMEGMAEYMSLGKVTPETAMWLRDAALQNKLPTIEQLTYDPRIFPYRYGHAL
                     WAYIGERWGDEAIGDILRGTVEGGGGIEGSIKRVTGLSLQQLSVQWRDAVNAKYLPEV
                     GSRVRARAVSQDLLDEKKDRGTLHLAPALSPDGSQVVYFGEHDWFFVDMWLADGKTGE
                     TKRRILKSTQSSNYETYRFINSQANWSADGKYLAFAAKRGPRDVIVIADVKRNHEVKQ
                     IDVKLNGITTPSWSPDGERLVFTGYDGGITDLFIVNSDGTGLKRLTEDKYADLHPVWS
                     PDGKTIAFATDRGPKTDFRTLKYGNYRIALYHLDTGVIDVLPHMDEGKNVSPQWAPDG
                     QSLAFVSDRNGVSNVFL"
BASE COUNT         1085 a         2015 c         2133 g         1144 t
ORIGIN      
        1 gcggaagagg ctccggcatt ccgctcgctc aatctgctca cgagcaaacc cgtgctctac
       61 gccgcaaacg tgtcggagtc ggagatcacc gaaggcaacc gcttcgtgga tgcactggtc
      121 tccgccgtgc agcgcgatgg cgaacaagcg gaagtcgtga cgttctcggc gaaagtcgaa
      181 gccgagttgg ccgagctggc gccggaagac cggaccgagt tcctcgcctc gctcgggctc
      241 aaggaatcag ggctcgaccg gctcgcccac gccgcgtacc atctgttggg tcttcagagc
      301 tacttcaccg ccggtgagaa ggaagtccga gcctggacta ttcatcgggg tgaccgggcg
      361 ccggaggccg ccggcgtgat ccattccgac ttcgagaaag gcttcatccg tgcggaaact
      421 gtgggttacg acgatttcgt gcgggtgggt ggctggaagc cggcccgcga acagggccta
      481 gcacgagccg aaggcaagga gtacgtggtg caggacggcg acgtgatgct cttccgattc
      541 agtccgtgac cctctatact tccgcgcgtc aacaacttcc ctcttcggct cgacaccaga
      601 gccggagcgc tcagaccaaa ggacggtgac atgacccgcc agtacgaggt ggtctacatc
      661 ttcgacagtg cgctcgaaga agcggccatc aacgagcgac tcgcccggta ccataccctc
      721 atccagcaac ccggtgtcga aacaccccag gtatcccatt gggggaagcg caccttgtcg
      781 tatcccatca agaagcacga gacggggtac tacgtcgtcg ccaagttcga taccgacggt
      841 gccgcgctcc ccgagttcga gcgcgccgtg aagctcgacg agtcagtgct tcgctttctc
      901 gtcgtcgtga acgagggtgc ccagccggtg cccgtcacta ccgccaaggg cgacgaggag
      961 gacgacgaat gaggcggcct cagaagactt gcgcgatctg cgagtcgggc gttcggatcg
     1021 tggactacaa ggatgagcgc acgctgcagc gattcctcac cgagcgtggc aagattctgc
     1081 ccagccggct gtcggggacc tgtgcacggc atcaacgcca gttgagcacc gcgatcaagc
     1141 gggctcgcca gctcgcactg ttgccgtaca tcaagggctt cggcggctga cgcaggtcgc
     1201 cggaatgagc ggtctgccgc ccggtcgccg cgcgcgtccg ggcgtgttgg ctttggcctt
     1261 ggcgtatctg ttgctcgccc cgcccggctt cctgcttggg cctctggcgg gactgctcgc
     1321 tttatcgcgg ccaactacgt tccgcgaatg ggcttggctg gggctcgcca ccggttggat
     1381 gtgcgtctgg ctggttccgg cgggcaatct gatggatcag ctcctgcgcg cgactgccgt
     1441 gctgatcacc ggcggcttcc tcgccatcac tctggtcggc agggcgcccg tgtttcggcg
     1501 cggtctggca gctctgggct ttgcaacagc ggcgctggtc ctgtgggcct ccgcatttca
     1561 cctggcgtgg tccgacctca aagcggtcat cggccgcgat gccgcccgca gcttgagcgc
     1621 cctggcgagc caggccgcga ccgccgggag cgacgaggcc gccgagctgc tgcggcagat
     1681 ggcaaccagc gccccgtcga tggcgatgct cacccctgcc ctggtcttcc tgagcggatt
     1741 gctgggcacg ctgctcgcct ggaacctcta tcaggggatc gccgtgcggg cgatggggga
     1801 cccgccggca cctttcccga gctttcggtt cagcgatcag atggtctggc ttccggtcgc
     1861 aggcctggca ctggtgctct ttccaccagt acccgccttg ggcgaggtgg gcgcgaacct
     1921 gctgctcgtc gcagtggctc tgtatgcggc ccgcgggctg gctgtggtgg gcgccggggc
     1981 gggtcgggtt ccccggctta cgaccacgtt cgtcgcgctg atcgccttct ttcttttacc
     2041 gtttgttgtc ggcgggctta cgctccttgg cctggccgac acgtggctag atttccgggg
     2101 acgcccggcg tcttccggca ccggaggatt cgaccgatga tcgaagtgat cctgcgtgaa
     2161 gatgtgaagt cgctcggcag ggctggagag ctggtccggg tgcgacccgg ctacgcccgc
     2221 aacttcctgc ttcccaaggg gctcgccttt gaagccaccg agggcaacaa gaagcggatc
     2281 gcggccgagt cgaaagcccg gagcggccgg gtcgaggcgg agcgcacgga ggcccaggcc
     2341 ttcgcgaaca ggcttgcgga gatcacgttg acggtggccg gcaaggcggg cgaagagggc
     2401 aagctgtttg ggtcgatcac ggctcacgac attgccgagg ggctggcgaa gcagggggtc
     2461 gaggtagacc gccggcgcat cgagctgggc cagccgatca agaccctcgg gtttcacacc
     2521 gtggcagtgc ggttgcaccc tgatgtccac gcagaggttc gggtccaagt cgtcggcgag
     2581 taacctggcc atgacggtcg ggatcgccta tctcgatggg ccgcggctcg cccgctcgct
     2641 cttcgccgcc gccgactggg tggcggccgg ccgcgacgag atcaatcgga tcaacgtctt
     2701 ccccgtccct gacggggaca ccggcaccaa cttcagcctc accttacgcg ccgtcgccga
     2761 tgcgctccgt gcgctcggcg acgcatcgct tccggagacc gccaacaccg ccgcccgtgc
     2821 ggccgtcctc ggcgcgcgcg gcaactccgg catgatgctc gcccatttcc tgatgggatt
     2881 tgccgaggca atcggagacc gacatcacgc gaccacgacc gatatcgcag cggcgctccg
     2941 ccggggcgcc gatcggctgt acgagtcgct ggacgatcct cgcgagggaa ccattctcac
     3001 cgtcgcgcgc gaggcggccg ctgccgccga gcgggccgcg gcggactgtg aggacatcag
     3061 cgaattcatg agccgtctgt tggccgaagc ggagcaagct ctggctcgga cgccggagct
     3121 gatgacggtg ctcaaggaag cgggagttgt ggacgccggc ggcaagggct tcgtgcggat
     3181 gatcgaaggc gtagtccgct tcatcgaggg cgatccgatt cttcccgccg cggccgaggt
     3241 cggtccgccg ataacgattc ccgcagcgct gatcggagtc gccgccgagc gcgacttcca
     3301 gtactgcacc gaggtgctgg tccggggagc acagctgccg ccggccaacg aagtgcgggc
     3361 ggcgatgcac aacttcggcg gttcggtgca ggttgccgtt atgagcgatg tcctcaaggt
     3421 ccatgtgcac actgatacgc ccgaggccgt ctttagttat gccgcgcgct gggggcggat
     3481 cgagaccacc aaggccgaag acatgcgagc ccagcatcgg aagctctcgc acgacactcg
     3541 gcgcccggtt gcggtggtgg tggacagctc ggcggacctc gcggacgcca tcctggaccg
     3601 gcaccacatc gcgatcgtgc cgctccaggt cgtcttcggc aacgaaacct ttcgtgatcg
     3661 cgtagaactg agaccggacg agttctatcg ccgcctccgg atcgcgaagg atctgccgac
     3721 cacgtcccag cccgctcccg ccgagtttgt gcgggttttc cgggacgctc tgcaggaggc
     3781 ggatgaagtc gttgcggtgg tgctggcggg cgctctgtcg ggaacacatc aggccggcgt
     3841 ggcggcagtc aaggcagcag ggttatccaa cgttatcgtg gtggatagcg ccagcgtgtc
     3901 gctcggagcg ggcatgctgg cgctgcgagc cgctgagctg gccgagtcgg gctggcggtc
     3961 gccggacatc gccaccgagt tggagcggct caagggtcag tcgggcgcgc tcatcacggt
     4021 ggaccagtac gacaacctgc tccggtcagg tcgggtgacg cggggcaaag catgggtcgc
     4081 cagcctgctg gacgtgaagc ccatcctctc gctggactcg gcggggaggg tggtgccggt
     4141 cgatcgggtt cgcggacgag acaacgtggc ccccagagtc ctgtcccttc tggagcgccg
     4201 tcttacccct cgtcccaaag ccgtgcggtt cgggatcgcc catgcagacg cgccggacgt
     4261 tgccgagcgc gtacgcacgg cgctggttgc ggcataccgg ccgcgagact gcttcgtctc
     4321 gatggccacg ggggtgctcg ggacccatgt gggggaaggc gcctggggcg tcttctatca
     4381 gatagaggac ggcactccga ccctgctgga gcgaggcaga gtgagcggag actggccggc
     4441 gtgacgcctc cgttcccgga ggcgctgggg cttgcgctcg aagccattga cgatctgaaa
     4501 ggcgtgcaac ctgtggtgct ggacctgcgc ggcttgagta atgcaaccga ctttttcgtg
     4561 attgcctccg gcacatccga tgcacacgtg cgcggcatcg cggagaacgt gatggagcgg
     4621 ctcgggcggc ggggtcaccg gacccatcac gtcgaagggc tgggcggtcg ctgggtgctg
     4681 ctcgatttcg tggattttgt gattcacctc tttcacccgg agacccgggc cttctatcaa
     4741 ctggagcgac tgtggggaga cgcccccgag ctcctgatcc ggtcttcgta aggggttcga
     4801 cctatggttc gtcgctgcac cgtggccctg gccctccttc tcggaacacc tgcgctgttg
     4861 gaggcccagt acttcggcca gaacaaggtc cagtacgaat cgttcgactg gaaaatcatc
     4921 cagaccgaac acttcgacgt gcatttttac cccgctgaac gcgaggcggc catggacgct
     4981 gcccgcatag ccgaacggtc gtatgcgcgg ttgtcgaaag tcctcggcca ccgcttccgg
     5041 gagcggaagg tgatcatcct ttacgcctcc cactccgatt tctcgcagac caacaccacg
     5101 gaaggagacg tgggcgaggg caccggcggc ttcaccgact ttttcaagca gcggaacatc
     5161 atgccgctca ccggagccta cgccgatatc gatcacgtcc tcacccacga aatgacgcac
     5221 cagttccagc tggacgtctt ttccagcggg cggggcggct cggcgctcca gggcgtgctc
     5281 cagcttcagc caccgctctg gttcatggag ggcatggcgg agtacatgtc gctcgggaag
     5341 gtcacgcctg aaaccgcgat gtggctccgg gacgcggcgc tccagaacaa gctccctacc
     5401 atcgagcaac tgacctatga cccgcgaatc ttcccctacc ggtatggcca tgctctctgg
     5461 gcctacatcg gcgagcggtg gggtgacgag gctattggcg acatcctgcg gggcaccgtg
     5521 gagggcggcg gcgggatcga gggctcgatc aagcgggtaa ccggtctcag tcttcagcaa
     5581 ctatcggttc agtggcgcga cgcggtcaac gcgaaatacc tgcccgaggt tggatcccgg
     5641 gtgcgcgcgc gtgccgtgtc gcaggacctt ctcgacgaga agaaggatcg ggggactctg
     5701 cacctggcgc cggccttgtc gccggacggg agtcaggtgg tctacttcgg cgagcatgac
     5761 tggttcttcg tcgacatgtg gctggcggat ggaaagaccg gcgagaccaa gcggcggatt
     5821 ctcaagtcca cccagtcgag caactacgag acctaccggt tcatcaactc ccaggccaat
     5881 tggtcggctg acggcaagta tctcgcattc gccgccaagc gcgggccccg cgacgtcatt
     5941 gtcatcgccg acgtgaagcg aaaccacgag gtgaagcaga tagatgtaaa gctgaacggc
     6001 atcaccactc cctcgtggag cccagacggc gagaggctgg tattcaccgg gtacgacggg
     6061 ggcatcaccg atcttttcat cgttaacagc gacggcaccg gcttgaagcg gctgacggaa
     6121 gacaagtacg ccgatctcca tccggtctgg tcccccgatg gcaagaccat cgctttcgcc
     6181 acggatcgag gaccgaagac ggactttcgg actctgaagt acggcaacta tcgcatcgcc
     6241 ctgtatcacc tcgataccgg cgtgatcgat gtgctgccgc acatggacga aggaaagaac
     6301 gtgagcccgc aatgggctcc ggacggacaa tcacttgcgt tcgtctcgga ccggaatggg
     6361 gtgagcaacg tcttcct
//
LOCUS       DASRJQ010000372         2379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3847962, whole genome shotgun sequence.
ACCESSION   DASRJQ010000372 DASRJQ010000000
VERSION     DASRJQ010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3847962"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VFB89_08325"
     CDS             <1..633
                     /locus_tag="VFB89_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HZM27349.1"
                     /translation="EWGWGTDDSEQALGLLKRFGSVSVLNGHIHQILQKVEGNVRFHT
                     AMSTAFPQPAPGAAPSPGPMKVEPGRLQKVLGISSVEFLRGHQALAVVDSALDGTPAL
                     PIAAGAAAAGPASRDSARGDTTVSGTATTIRIDNFSFIPATTTVPVGSAITWVNADDV
                     PHKIVSSDGKFTASPAIDTNDRYTFRFTQPGRYEYFCALHPKMTGTIEVR"
     gene            complement(638..2266)
                     /locus_tag="VFB89_08330"
     CDS             complement(638..2266)
                     /locus_tag="VFB89_08330"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF006006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZM27350.1"
                     /translation="MNPHNDQASLEVTIGRRAFLQLGTAFGAAMALPPSPVAAGTVTG
                     QTRALNAKPFQEPFELDEVTISQLQASLGSGKHTARSLAELYLRRVDEVDSGGPAVHA
                     IIERNPDALADADALDAERRTRGPRGAFHGIPVLVKDNIDTADKMRTSAGSLALAESV
                     AGQDAPLVRRLRAAGALILGKANLSEWANFRSTHSVSGWSGRGGLTRNPYALDRNPCG
                     SSSGSAAGVSANLAAVAVGTETDGSIVCPASACGVVGIKPTVGLVSGAGIIPISKSQD
                     TAGPMARTVADAALLLGVLANKDFSSALDPAGLEGARIGVARNLFGFNDRVDRVLEEA
                     LSAMREAGARLVDPAKLPTGKQLEDAELDVLLYEFKDGLDKYLAGLGPETPARSLAAL
                     IDFNEQHRDREMPYFGQELFLQAQAKGPLTDRAYRQALATCRRLSRAEGIDATLRKHR
                     LAAIVAPTGGPAWVTDLLNGDHFTGGSSTLPAVAGYPAITVPAGFVSGLPIGITFMGP
                     ARSDWRLIKLAYAFEQATRLRRKPSLLPTADLSS"
BASE COUNT          413 a          769 c          795 g          402 t
ORIGIN      
        1 gagtggggct ggggaaccga cgacagcgag caggcactcg gcctgctcaa gcgtttcggc
       61 tccgtcagcg tgctcaacgg ccacatccat cagatcctgc agaaggtgga gggcaacgtg
      121 cgctttcaca cggcgatgtc caccgcgttc ccccagccgg cgccgggtgc cgcaccctca
      181 ccgggcccga tgaaggttga acccgggcga ttgcagaagg tactgggcat ctcgagcgtc
      241 gagttcctgc ggggccatca ggcgctcgcg gtggtggact cggcgctgga cggcacgccc
      301 gctctaccca tcgcggcagg cgccgcagcg gcaggtccgg cgtcacggga ctctgcacgg
      361 ggagacacta cagtcagtgg gacggcgacc acgatcagga tcgacaactt cagcttcatt
      421 ccggcgacga ccaccgtacc cgtcgggagc gcgatcacct gggtcaatgc agacgacgtg
      481 ccgcacaaga tcgtgagcag cgacgggaag tttacggcat caccggcgat cgacaccaac
      541 gaccgctaca cgtttcgatt cactcagccg gggcggtacg agtacttctg cgcgctccac
      601 ccaaagatga ccgggacgat cgaggttcgg tgagtgctca gctggaaagg tccgcggtcg
      661 gcagaaggct cggcttccgc cggagccggg tcgcctgctc gaaggcgtac gcgagcttga
      721 tgagacgcca gtcgctccga gcgggtccca tgaaggtgat gccgatgggc aagccgctca
      781 cgaacccggc tgggacggta atggccgggt atccggcgac ggcgggtaag gtgctgctgc
      841 cgccggtgaa gtggtcgccg ttcaggaggt ccgtcaccca ggccggccct cctgtcggtg
      901 cgacgattgc cgccagcctg tgcttgcgca aggtcgcatc gatcccttcc gcgcgcgaaa
      961 gcctgcggca ggtggctaat gcctggcgat acgcccggtc ggtgagcggt ccctttgcct
     1021 gggcctgcag aaacagctcc tgtccgaagt agggcatttc gcggtcgcgg tgctgttcgt
     1081 tgaagtcgat gagcgctgcc agcgatcgcg ccggcgtttc cggaccgagc cccgccaggt
     1141 acttgtcgag cccgtccttg aactcgtaga gcagcacgtc cagctcggcg tcctcgagct
     1201 gcttgcccgt cggaagtttt gccggatcca ccaaccgagc tcccgcctcc cgcatggcgc
     1261 tgagcgcctc ctccagcact cggtccaccc ggtcgttgaa tccaaacagg tttcgcgcga
     1321 caccgatccg ggcgccctcg agtcctgcag gatcgagcgc ggacgagaag tccttgttgg
     1381 ctaggacacc gagcaacaag gccgcgtcgg cgacggtgcg ggccatcggg ccggcagtgt
     1441 cctggctttt tgagatcggg atgattccgg cgccgctcac gagtccaacc gttggcttga
     1501 tcccaacaac gccacaagcc gaagcagggc agacgatcga cccgtcggtc tcggttccta
     1561 ctgcaacagc agcgaggttg gcggaaacac cggcggccga gcctgagctc gacccgcaag
     1621 gattccgatc cagcgcatac gggtttctgg tcagtccccc gcgcccgctc cagccgctca
     1681 ccgagtgggt cgatcggaag ttggcccatt cgctgagatt ggccttgccg agaatcagcg
     1741 caccggccgc gcgaaggcga cgcacgaggg gcgcgtcttg ccccgcgacg gactctgcca
     1801 acgccagcga gccagcgctg gtccgcatct tgtcggcggt gtcgatattg tccttgacca
     1861 gaacaggaat gccgtgaaat gccccgcgag gtccccgggt tcgccgctcg gcatccagag
     1921 catcggcgtc ggcgagtgcg tcggggttcc gctcgataat ggcgtggacc gcggggccgc
     1981 cgctatccac ctcatcaacg cgtcggagat acagctcggc gagcgaccta gcggtgtgtt
     2041 tgccggatcc cagggatgcc tgcagctggc tgatagtgac ctcgtcaagc tcgaacggct
     2101 cctggaaggg cttggcgttg agagcgcgcg tctgcccagt aacggtgcct gctgccaccg
     2161 gcgatggagg caacgccatg gcagcgccga aggcggtacc gagttggaga aaagctcgac
     2221 gcccgatcgt gacctcaagc gaggcctgat cgttatgagg gttcattggg tttcgctctg
     2281 gtgggagaag tccaggcgac cggcgttcgt tatcccggcc agtccgccgc caaattggac
     2341 agcaccacga cgccggcgtt gctctagtag ccgggcggg
//
LOCUS       DASRJQ010000373         3654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3950600, whole genome shotgun sequence.
ACCESSION   DASRJQ010000373 DASRJQ010000000
VERSION     DASRJQ010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3950600"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VFB89_08335"
     CDS             complement(<1..617)
                     /locus_tag="VFB89_08335"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HZM27351.1"
                     /translation="MTKHKTGSRKEWLAARLELLEAEKELTRRSDEVAQRRRDLPWVR
                     IDKAYRFETDEGSASLADLFRGGSQLLVYHFMFGPDYTAGCPSCSSIADGFDGFGVHL
                     ANHDVMLWAVSRAPLPKLQGYKRRMGWTFPWASSFGGDFNFDFGVGFTEEQQREGGIE
                     YNYRREAGAPLVGRDTASWREPSGEGVVAQVAAMAGTDEATFTRER"
     gene            775..3096
                     /locus_tag="VFB89_08340"
     CDS             775..3096
                     /locus_tag="VFB89_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM27352.1"
                     /translation="MFRWLKKTPPPVSTPPESATATATPQPEPQVLRHYRIGRKLGEG
                     GMGVVYEARDERLDRSVAIKRMRFQDASLRERLLREARTAAAIAHPNICQVYELGEEG
                     GELYIVMELLAGETLASRIGGGPMPLGEALQTALGVLGALEALHARDIVHRDLKPSNI
                     FLTQHGVKLLDFGVARPQATASLDPGLTLPGVIIGTPSYMAPETVDNEPAGPQADLFA
                     VGAILFEMLTGKRAFNGNTVLEIQNAVVREHPPALVGGADVMAADRVIQRALAKRLEE
                     RYADAATMARDVREALTLIDTGPAPRVRTMTRLVVLPFRMLRPDPELEFLTHGLPEAI
                     SASLAGLETMAVRSSAAAERYADEHPDLKLIAAETGVDAVVIGSLLRAADQVRVSIQL
                     VEVPSGTLVTTRTAQVALTDIFQLQDELTRQIVDALAIPLSAHDRQVLAQDAPTRPEA
                     YELYLRANHLSEDASHVRNLTTARELYRRCLELDPTFAPAWARLGRVHRVMAKFGHAP
                     YAEESPQAEQAFRKALELNPDLPLTHNLYTNFEIEEQGRAREAMVRLLRQAEKRTVDP
                     DLLTGLVIACRFCGLLQASLAADRRARRIDPAVRTSVAYTHWLLADYQQTMLTDLEAF
                     QGLRLACLWMLGRKAEALEASRHLRSYEAEGAEHWYLKAQLAAFQGDRETCIAATRQV
                     LATGFHDPEGLLFGARDVAYVGDTDFALDILTRVVDGGMHCPAPLVRDPWFDSLRTEP
                     EFVRLLRRLEQEQAESLRVFIQAGGEQLLGVAG"
     gene            3108..3383
                     /locus_tag="VFB89_08345"
     CDS             3108..3383
                     /locus_tag="VFB89_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27353.1"
                     /translation="MERLGLAVLAILFALAIALWLYGVYCYVQMVRHRAAGSSPFQLA
                     WPSQHLTERGREFRRRALRAYAAFGLLALLLLVLATLLGTWFRRRAV"
     gene            3380..>3654
                     /locus_tag="VFB89_08350"
     CDS             3380..>3654
                     /locus_tag="VFB89_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27354.1"
                     /translation="MNRQVRPATPCALLVSLSFALINTPSLRCQVEYAAPIMGGISNH
                     GQNLGQPYVTAGDRAYLIGTQDGNFPDMGEHVPGEMAGLWLHPIKLI"
BASE COUNT          606 a         1234 c         1164 g          650 t
ORIGIN      
        1 cgctcgcggg tgaaggtcgc ctcgtcggtt cccgccatcg ccgcgacctg ggccacaacc
       61 ccctcgccgc tgggttcccg ccacgatgca gtgtcccgtc ccaccagcgg ggcgccggcc
      121 tcgcgccggt agttgtattc gatgccccct tcgcgttgtt gttcctcggt gaacccgacg
      181 ccgaaatcga agttgaagtc gccgccgaac gacgacgccc agggaaaggt ccagcccatc
      241 cgtcgcttgt acccctgcag cttcgggagc ggcgcccgcg acaccgccca cagcatcacg
      301 tcgtggtttg ccagatgcac gccgaagccg tcaaacccgt ccgcgatcga tgagcaggac
      361 ggacagccag cggtgtagtc gggcccgaac atgaaatgat agaccaggag ttgcgagccc
      421 ccgcgaaaga ggtccgccag cgacgcgctc ccctcgtcgg tctcgaagcg atacgccttg
      481 tcaatccgca cccagggcag gtcccgccgc cgctgcgcca cctcgtcgct gcggcgcgtg
      541 agctccttct ccgcctcaag caactccagc cgcgccgcca gccactcttt acggcttccc
      601 gtcttgtgtt tggtcatcgt tgcgctcatt gggtgtcctt cctcgttgtt ccgttcgcgt
      661 cacgacgtgc tggtccatgt cgtcaggtac attaacaact ggccgacgaa tgcagttagc
      721 caccccggcc cgacggtact tgatttttgc gactgaccga gcgacggtcc gccaatgttt
      781 cgctggctga agaagacacc acccccggta tcgacgcccc ccgaatcggc gacggcgacg
      841 gcgaccccgc agccggagcc gcaggtactg cgccactacc gcatcggccg gaagttgggc
      901 gagggtggca tgggcgtggt ctacgaggcg cgtgacgagc gtctcgatcg ctccgtcgcc
      961 atcaagcgca tgcgctttca ggacgcgagt cttcgtgagc gcttgctgcg cgaggcccgc
     1021 acggctgcgg ccatcgcaca tcccaacatc tgtcaggtgt atgagctggg cgaagaaggc
     1081 ggcgagctct atatcgtcat ggagctcctg gccggcgaaa cgctagcgtc tcggattggc
     1141 gggggaccaa tgccgctggg cgaggcgctg cagaccgcgc tgggagttct cggcgcactc
     1201 gaggcgctgc atgcccgcga catcgtccac cgggacctca aaccgtcgaa catttttctc
     1261 acccagcacg gcgtgaagct gctcgatttc ggggttgcgc gtccgcaggc cactgcgtcg
     1321 ctcgaccccg gcttgacact tcccggcgta atcatcggca cgccgagcta catggcgccc
     1381 gagacggtcg acaacgagcc cgcgggcccg caggcggatc tgttcgccgt gggcgcaatc
     1441 ctgttcgaga tgctgaccgg gaagcgggcc ttcaatggga acacggtcct cgagatccag
     1501 aacgcggtgg tccgcgagca cccccctgcc ctggttggtg gcgccgacgt gatggcggcc
     1561 gatcgggtga tccagcgcgc actcgccaag cgcctggaag aacgatacgc cgacgcggcg
     1621 acaatggctc gcgacgtacg cgaggcgctc acgctgatcg acaccggccc ggcaccgcgg
     1681 gtccgcacca tgacccggct ggtcgtgctg cccttccgta tgctccggcc cgatcccgag
     1741 ctcgagttcc tcacccacgg cttgcctgaa gcgatctcgg cgtcactggc cggcctggaa
     1801 accatggcag tgcggtccag cgccgctgca gagcggtacg ccgacgagca ccccgatctc
     1861 aagttgatcg ccgccgaaac cggggtggac gcggtggtga tcggcagcct gctgcgcgcc
     1921 gccgaccagg tccgggtctc gatccagctg gtcgaggtgc cgagcggtac gttggtcacg
     1981 acccgtacgg cccaggtagc gctcaccgac atcttccagc tgcaggacga gctgacccgt
     2041 cagatcgtgg acgcactcgc gatcccgctc tccgcgcacg accgccaggt gctggcccag
     2101 gatgcgccta ctcgccccga ggcctatgag ctgtatctgc gggccaatca cctgagcgag
     2161 gatgcttcgc acgtcagaaa cctgactacg gcgcgggaac tgtaccgccg gtgcctcgag
     2221 ctggacccga cctttgctcc ggcgtgggcg cggctcggac gggtgcatcg ggtcatggcc
     2281 aagttcggac acgcgcccta tgcggaggag tcgccccagg ccgagcaggc ctttcgcaag
     2341 gccctcgagc tcaaccccga ccttccgctc acgcacaacc tctataccaa tttcgagatc
     2401 gaagagcagg gtcgcgcgcg agaggcaatg gtgcgcctgc tccgccaggc cgagaagcgg
     2461 acggtggacc ccgatctcct gacagggctg gttatcgcgt gccggttctg cggtctattg
     2521 caagcgtcac tggccgcaga ccggcgggcg cggcggatcg accccgcggt ccgcaccagt
     2581 gtggcctaca cgcactggct actcgccgac taccagcaaa caatgctcac cgatctggag
     2641 gcatttcaag ggctgcggct tgcatgcctc tggatgctgg ggcgcaaggc cgaggccctc
     2701 gaggcatccc gtcaccttcg atcgtacgaa gcggagggcg cggagcattg gtatctcaag
     2761 gcccaactcg cagcatttca gggtgaccgg gagacatgca ttgctgctac ccggcaggtg
     2821 ttggcgactg gttttcacga cccggaagga ctgctgtttg gggcgcgcga cgtggcgtac
     2881 gtcggtgaca cggatttcgc gctcgacatc ctgacgagag tggtggatgg cgggatgcac
     2941 tgccctgcac cgctggtgcg ggatccctgg ttcgactcgc tccgtacgga gcctgagttc
     3001 gtacgtctgc tccggcggtt ggaacaggag caggctgaat ccctccgcgt ctttattcag
     3061 gcaggaggcg agcagttgct tggtgtggcc ggttagccgc cgaagaaatg gagcggctcg
     3121 ggcttgctgt actagccatc ctcttcgcgc tagccatcgc cctctggctc tacggcgtct
     3181 actgctacgt ccagatggtg aggcatcgtg ccgcaggcag cagcccattc cagctcgcct
     3241 ggccatccca gcaccttacg gaacgtgggc gcgagtttcg gcgacgggcc ctccgggcct
     3301 atgccgcctt cggactactg gcgttgctgc tcctggtcct ggccactctg ctgggaacgt
     3361 ggtttcggcg gagggctgtg tgaaccgaca ggttcgacct gcaacaccct gtgccctcct
     3421 ggtgtcgctc tccttcgccc tgatcaacac tccctcgctg cggtgccagg ttgagtacgc
     3481 ggcgcccatc atgggaggta tcagcaatca cggccaaaac ctgggtcagc cgtacgtcac
     3541 cgcaggcgac cgagcgtatt tgatcggaac tcaggacggg aatttcccgg acatgggcga
     3601 gcacgtcccg ggcgaaatgg caggcctctg gctgcatccg atcaagctga tcga
//
LOCUS       DASRJQ010000374         5487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2551604, whole genome shotgun sequence.
ACCESSION   DASRJQ010000374 DASRJQ010000000
VERSION     DASRJQ010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5487
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2551604"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VFB89_08355"
     CDS             <1..745
                     /locus_tag="VFB89_08355"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZM27355.1"
                     /translation="VHAAAVREHEAVSGAIESLARASGLPSRTLDSIPLGMDPQPTCP
                     VSDTDSLQALALRQRPEVGRGLAEYAVAEGELRVAVANAYPDLSLGPGFVWDQGVNRW
                     SLLLGLPHIAFNRNRGPIGEALARRQEAGARFSERQQAVLEEVSAGVAACRLALTDVS
                     GAESVREATARQAQLARAAYDRGELSATALEPLELAETRALRDLHGARQRLAAAGLAI
                     ETAIGAWPPGEVRWPDLRKPTRPVQEASH"
     gene            745..1218
                     /locus_tag="VFB89_08360"
     CDS             745..1218
                     /locus_tag="VFB89_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27356.1"
                     /translation="MRWFVRLLASAAVVVLAVYLVATYRRHQAGLAAEAAREEPVIAP
                     SRVKDVQGEAVIVLDSGDVARLGLRTVVLRSSSSTPIRRLAGELIPEPDRITTVRAPI
                     AGRLSVPPNARWPGFGDRLTAGREIAQVVPAPRDRSRSRARAGRLRSQCPRRRQA"
     gene            1124..4225
                     /locus_tag="VFB89_08365"
     CDS             1124..4225
                     /locus_tag="VFB89_08365"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZM27357.1"
                     /translation="MRRSFRHRGIVVGLALALVVYGLNVLAGAKLDVFPEFAPPQVSI
                     QTEAPGFSAEQVEILVTKPIEDAINGVNGIAMVRSQSIQGLSVITAVLAEPTDIYRAR
                     QSLAERLGEAQNRLPRTVPPPVISPLTSSSSTVLVVGITSRTRSLMEQRTFVDWTLRP
                     RLLAAPGVAKVAVFGGEVRQLQIQLDPERLRLYQIGVPEIIAAASRATGVRGAGVLDT
                     PNQRVVVRTEGQLLAPADLARSVVRERAGAVLRLGDLGRVVEAPEARFGEGGINGERG
                     LVIVVSAQLGANTKIVAAGAEQALHALGPAIAAAGLTLHPALFRPSEFIDLALRNITN
                     SLLLGAALVAIVLFVFLADVGAAAISLTAIPLSLLTAIIVLDAFGFGINTLTLGGLAI
                     ALGEVVDDAIIDVENIARRLRQHRAVGAASIRSVAEVVLDASLEIRRSVVYATFVVAL
                     VFLPVLALSGVQGALFRPLGLAYILATLASLGVALTVTPALTLILLGRREHRQHESAF
                     LAWLKLGYRRALGWLCDRPVGVGLAALTAFLAAAATLPFFGATFLPEFREGHYLIHMS
                     AVPGTSLAETFRLGQQVTAALRADPRVRSVAQRIGRAELSEDTWGTHYTEFEVDLVPV
                     SGADAETVQEDLRRTLAKFPGVNFAIRGFLAERIEETLTGSTAEIVVKVFGDDLDSLD
                     VAARRIAQTVRAVPGASDVQYDPPAVTPEVTIRVRPNDLSAAGLASDDVLAAVEAATR
                     GVKVGQTFEGNRSTDVVVILEPEARGQPEALRNLPVVSEGGRMLSLAQVADVSRTTGR
                     FLISHQAARRVQTATANVTGGDVAGFAEALERKLASTRLPAGVYSELGGTATARREAQ
                     RELLLRGLLAGVGIVMLLALAFGNPSRLLLVLANLPFAMVGGVLAVFATGGLLSLGSL
                     VGFVTLFGIATRNAIMLISHYDHLVRLEGETWGKEAAIRGALERLGPILMTALVTALA
                     LVPLAVGSGDPGREIEGPMAIVILGGLLTSTLLSLFVLPTLALRWGRFEGIQSAPVDG
                     ARQ"
     gene            4364..4813
                     /locus_tag="VFB89_08370"
     CDS             4364..4813
                     /locus_tag="VFB89_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZM27358.1"
                     /translation="MSDRPILSTPIAGAQTILVVDDVRIVRQVIYRLLSQVGYRVFEA
                     ASGAEAIEVLSTANRPIDLVVVDVVMPKMSGVMLARAIHERYPGMRILFMSAYPAEVM
                     VREGLEHPNVIFLAKPFSGGELLNKVTAALRQEPSVSIPRPTVKQPD"
     gene            complement(4810..>5487)
                     /locus_tag="VFB89_08375"
     CDS             complement(4810..>5487)
                     /locus_tag="VFB89_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZM27359.1"
                     /translation="PQLFEPFFTGADVSTHCSGKFEHGRKGIGLGLSVARSFVRMHGG
                     TILAASEPGQGSTFKIYLPLVEGRVAHVEQPPISNGNLRGNETIAVVEDDEVVRTLLA
                     RMLRDYGYRVLEAADGQSALDLLRHNPNGVDLVVSDVVMPKMGGQELGESLSARGQDV
                     PVLFISGFTGEDVVRRGLLKPGNPFQEKPFTPELFVERVRAMLDAPAGSGPARLMHIE
                     PSEARIS"
BASE COUNT          829 a         1798 c         1851 g         1009 t
ORIGIN      
        1 cgtccacgcc gcggcggtcc gggagcacga ggcggtgagc ggcgcaattg agtcgttggc
       61 gcgggcatcg gggcttcctt ccagaactct ggatagcatt cctctcggaa tggacccgca
      121 accgacttgc cctgtctccg atacggacag cctgcaggcg ctcgcgcttc gccagcggcc
      181 cgaagtcggc cgggggttgg cggagtacgc cgtcgccgaa ggcgagttgc gcgttgccgt
      241 ggcaaacgcg tacccggacc tcagcctggg gcccggattc gtctgggatc agggtgtcaa
      301 caggtggtcg ctcctgctcg gactgccaca catcgcgttc aaccggaacc gcgggcccat
      361 cggcgaggca ctggcccggc ggcaggaagc gggagcacga ttctccgagc ggcagcaggc
      421 ggtgctggag gaagtctcgg ccggtgtcgc tgcgtgccga ctggctctca ccgatgtgag
      481 cggggccgag tccgtgcgcg aggcaaccgc gcgacaggca cagctggcgc gagccgcgta
      541 cgaccggggc gagctcagtg ccaccgcgct cgagcctctg gagctggccg agacccgggc
      601 gttgcgggat ctccacggag ctcggcagcg cctggcggcc gcgggcctcg ctatcgaaac
      661 cgcaatcggg gcctggccac cgggcgaggt gcgttggccc gatctccgga aaccgactcg
      721 accggtgcag gaggcatctc actgatgcgt tggttcgtgc ggctcctcgc gagcgcggcg
      781 gtggtggtgc tcgcagtgta cttggtggcg acctatcgga gacaccaagc tggcctcgcg
      841 gccgaggcgg ctcgggagga gccggtcatc gctcccagcc gcgtcaagga cgttcagggt
      901 gaggccgtga tcgtgctcga tagcggtgat gtggcacgcc tggggcttcg caccgttgtg
      961 ctccgatcga gcagctccac ccctattcgc cggctcgccg gcgagctgat cccggagccc
     1021 gatcgcatca ctactgtgcg tgctcccatc gccgggcgtt tgtcggtgcc gccgaatgcc
     1081 cggtggcccg gcttcggtga ccgcctcacc gccgggcgcg agattgcgca ggtcgttccg
     1141 gcaccgcggg atcgtagtcg gtctcgcgct cgcgctggtc gtctacggtc tcaatgtcct
     1201 cgccggcgcc aagcttgacg tctttcccga attcgcccct ccccaggtct cgatccagac
     1261 cgaagcgccc ggattctcgg ccgagcaggt cgagatcctc gtcaccaagc cgatcgagga
     1321 cgcgatcaat ggcgtcaacg gcatcgcgat ggtgcgctct caatcgatcc agggcctttc
     1381 cgtcatcacg gctgttctgg ccgaacccac tgacatctac cgggcccgcc aatcgttggc
     1441 cgagcgcctg ggcgaggcgc agaaccggct gccgcgcacc gtgccgccgc cggtcatcag
     1501 tccgctaacc tcctcatcca gcaccgtcct cgttgtcggc atcacctcga ggacgcgctc
     1561 gttgatggag cagcgcacct tcgtggactg gaccctgcgt ccgcgcctgc tggcggcgcc
     1621 cggtgtggcc aaggtcgcgg tcttcggggg agaggtccgc caactccaga tccagctcga
     1681 tccggaacgc ctccgactct atcagatcgg tgtgccggag atcatcgccg cggccagccg
     1741 cgcgaccggc gtgcgaggcg caggggtgct ggatacaccg aaccagcgcg tcgtcgtgcg
     1801 cacggaaggg cagctgctgg cgccggctga cctggctcgg tcagtcgtgc gggaaagggc
     1861 cggcgccgtg ttgcggttgg gcgatctcgg tcgagtggtg gaagcgccgg aagcgcgctt
     1921 cggtgagggt gggatcaatg gagagcgcgg actggtaatc gtggtgtcgg ctcagctcgg
     1981 cgccaacacc aagatcgtcg ccgcgggcgc cgagcaagcg ttgcacgcac tcggacccgc
     2041 tatcgcagcg gccgggctca cccttcatcc cgccctcttc cggccctccg agttcatcga
     2101 cctcgccctt cgcaacatca ccaactcgct cctgctgggc gcggccctgg ttgcgatcgt
     2161 gctgttcgtg tttctggcgg atgtcggggc ggcagcgatt tccctgaccg cgatcccgct
     2221 gtcgctgctg acggcaatca tcgtactcga tgcgtttggg ttcgggatca acaccctgac
     2281 actcggcggg ctggcgatcg cgctcggcga agtcgtggac gacgcgatta tcgacgtgga
     2341 aaacatcgcc cggcgtttgc ggcagcacag ggcggtcggg gcggcatcga tccgaagcgt
     2401 cgctgaggtg gtgctggatg cgtcgctcga gattcggcgc agtgtcgtct atgcgacctt
     2461 cgtcgttgcg ctggtgttcc tgcccgtgct tgccttgtcc ggcgttcagg gcgcgctgtt
     2521 tcggccgctg ggcctggcct acatcctggc cacgctggcc tcgctcggcg tcgctttgac
     2581 cgttacgcct gcgctgactc tgatcctgct cggccggcgg gagcaccgac aacacgagtc
     2641 cgccttcctg gcctggctca agctgggtta tcgccgcgcg ctggggtggc tgtgcgaccg
     2701 gcccgtcggc gtcggattgg cggcactgac ggcgttcctg gccgctgccg cgacgcttcc
     2761 gttctttggg gcgacgttcc tgccggagtt tcgcgaaggc cattacctga tccacatgtc
     2821 ggcggtgccg ggaacctcgc tggccgaaac gtttcgtcta ggtcagcagg tgacggcggc
     2881 gctccgggcc gatcctcgcg tacgctccgt ggcgcaacga atcggacgtg cggagctgtc
     2941 ggaagacacc tggggcacgc actacacgga gttcgaggtg gacctggtcc cggtatccgg
     3001 cgctgacgcg gagacggtcc aggaagatct ccggcgcacg ctggcgaagt ttccgggcgt
     3061 caacttcgcg atccgcgggt tcctggccga gcggatcgaa gagacactca ccggctcgac
     3121 tgccgagatc gtggtcaagg tgttcggcga cgacctcgac agcctcgatg tcgcggcacg
     3181 ccgcatcgct cagacggttc gagccgtgcc gggagcatca gacgtgcaat acgacccgcc
     3241 tgcggtcacg cccgaggtca ccatccgggt gcgcccaaac gatctgtccg ctgccggcct
     3301 cgcatccgat gacgtgctcg cggcggtcga ggccgccacc cggggagtga aggtaggaca
     3361 gaccttcgag gggaaccgtt ccaccgacgt cgtggtcatc ctcgagcccg aggccagagg
     3421 gcagcccgag gcgcttcgca atctgccggt cgtctccgag ggtggtcgca tgctctcgct
     3481 ggcgcaggtg gcggatgtgt cgaggaccac gggccgattc cttatctcac accaggcagc
     3541 gcggcgggtt cagactgcta ctgcgaacgt caccggaggt gatgtggcgg gttttgccga
     3601 ggcgctcgag cggaagctcg cgagcacccg gctccccgcc ggcgtctact cggagctggg
     3661 tggaaccgcc acggctcggc gcgaggcgca gcgcgagctg ctgctgcgcg gcctgctcgc
     3721 cggcgtgggg atcgtcatgc tactcgcgct cgcgttcggc aacccgtcac ggctgctgct
     3781 ggtcctcgcc aatctgcctt tcgcgatggt cggtggggtg ctggccgtat tcgcgaccgg
     3841 agggctcctc tcgttgggat cgctcgtcgg ctttgtgacg ctcttcggca tcgccacccg
     3901 aaacgcgatc atgctgatct ctcattacga ccatctggtg aggctcgaag gcgagacctg
     3961 gggcaaggaa gccgcaatac gcggggccct cgagcggctg ggccccattc tcatgacggc
     4021 gttggtgacg gcgctggcgc tggttccgct tgcggttggg agcggcgatc cggggcggga
     4081 gatcgagggg ccgatggcca ttgtcattct tggcgggctc ttgacctcga cgctgctgag
     4141 ccttttcgtg ttgccgactc tggcgttgcg atggggccgc ttcgagggga ttcaatctgc
     4201 gccggtggac ggagcgaggc agtgaccgtc aggtcacaac gaccggatat gcagtgatcc
     4261 agcgcaccgc gacggccctt ctgaaattcg gtattgctct cttggtgacg gcgcaacggg
     4321 aagcttgagg acggcagaaa gacgagaact cattcaccgc acaatgtcag acagaccgat
     4381 actttccact ccgattgccg gcgcccagac aattctcgtt gtcgacgacg tccggatcgt
     4441 ccgccaggtc atttatcgac tgcttagcca ggtcgggtat cgcgttttcg aggccgcgtc
     4501 cggtgccgag gcaatcgagg tgctatcgac tgcaaaccgt ccgatcgacc tggtggttgt
     4561 cgatgtggtg atgccgaaga tgagtggtgt gatgctggcc cgcgccattc acgaacggta
     4621 ccccggcatg cggatcctgt tcatgtcggc ctatcctgcg gaagtgatgg ttcgcgaggg
     4681 gctcgagcac cccaacgtca ttttcctcgc gaagcccttc agcggcggcg agctgctgaa
     4741 caaggtgact gctgcgctcc ggcaggagcc atcggtgtcg attccgaggc cgaccgtcaa
     4801 acagcctgat tagctgatcc tcgcttccga aggttcaatg tgcatcagtc gcgccggccc
     4861 ggaaccagcc ggcgcgtcta gcattgctct taccctttcg acaaacagct ccggtgtgaa
     4921 cggcttttcc tggaacgggt tgcccggctt gagcaaaccg cggcgaacga catcctcccc
     4981 ggtaaacccg gagataaaga gcacagggac gtcctggcct cgggccgaga gtgattcacc
     5041 cagctcctgg ccccccattt tgggcatgac cacgtcggag actaccaggt cgacgccgtt
     5101 cgggttgtgc ctcagcaggt cgagagcgga ctggccgtcc gccgcctcca gcacacggta
     5161 gccgtaatcc cgcagcatcc gcgccagcag cgtacgtacc acctcgtcgt cttctacgac
     5221 agcgatggtc tcgttgcccc tgaggttgcc attactgatc gggggctgct ccacgtgggc
     5281 cacccgtccc tcgaccagcg gcagatatat tttgaacgtc gagccctgtc ccggctcgct
     5341 cgccgcgagg atcgtcccgc catgcatgcg gacgaacgac cgcgcgacgc tcagccccag
     5401 cccgattccc ttgcgcccgt gctcgaactt cccggagcag tgcgtggaca cgtcggcgcc
     5461 cgtgaagaac ggctcgaaca gctgcgg
//
LOCUS       DASRJQ010000375         4754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2474836, whole genome shotgun sequence.
ACCESSION   DASRJQ010000375 DASRJQ010000000
VERSION     DASRJQ010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2474836"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /locus_tag="VFB89_08380"
     CDS             <1..1270
                     /locus_tag="VFB89_08380"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZM27360.1"
                     /translation="IAASTNRDEPFPKDTESQIARFTELVATAVESAEAREELRRIAE
                     EQAALRRVATLVARGEEPAAVFEAVTEEIRGLFHADATALIRFESNGEATFLGGRGWL
                     RQRLPGTRFQPPHGFALASVREAGLVAHFGIDDVTSRTLLEEMRQEGVRYAVAAPIAV
                     EGLVWGAINVWSRRGPLPPDIEQRIVNFTELIATAIANADVRSKLTQSRARIVATADA
                     TRRRIERDLHDGAQQRLVSLALELRLALESVPAELPALGNSIRRVADELAAVQAQLRE
                     ISRGIHPSILSEGGLGPAVRTLARRSPVPVELDIQTSSRYPASVEVAAYFVVSEALTN
                     TAKHASGSHADVVVWETDGALRLRVSDDGVGGAQPQEGTGLTGLHDRVEALGGSIEVT
                     SPVGHGTVLEVSLPTDRPDWSASTGRSEAS"
     gene            complement(2114..2632)
                     /locus_tag="VFB89_08385"
     CDS             complement(2114..2632)
                     /locus_tag="VFB89_08385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZM27361.1"
                     /translation="MDVGSRQREAALQELHDKHASQLLQFAMRRTHDRQLSEDIVQEV
                     LLRAWKDPSLGQRDQAAARAWLYTASRNLIIDRWRSAASRYEQRMEDPPEESAGDMTS
                     VVLDRWLLTEALASLSTEQRSVINAANYEGRSIADISARLRIPEGTVKSRLHYGLRTL
                     RLALQEKGVTRP"
     gene            3225..3701
                     /locus_tag="VFB89_08390"
     CDS             3225..3701
                     /locus_tag="VFB89_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27362.1"
                     /translation="MANIRTGEENAGHIDGDYSDRGVVGPARQRSPILWRALSALLLL
                     TMGGIHLFLVLTGVGGLLGVLFVLNAIGGVVLAIAMIAAPRRFLPVASVLGLLFMAGT
                     LLALVLALTVGLFGIREQLGGELVPTTLIVESIGIIVLAVTTGLAFRMRSRTVASS"
     gene            3877..>4754
                     /locus_tag="VFB89_08395"
     CDS             3877..>4754
                     /locus_tag="VFB89_08395"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZM27363.1"
                     /translation="MSVSERNHRIVVAGAGYAGLHVAMRLATKLRDNPGVQLTLVDWH
                     DFHQVMTELPRVAGGTRAADAVRIPLDDVVARGVRFMETQITGFDLAGQQLKTQSGPI
                     GYTRLVLALGSRPNDFAIPGLAERAISLYSVADAEQVWAAVNQAVATAATATDPDQQR
                     RLATVVIGGGGATGVELAGELAEMLPELARAHRLAPDRPAVILVEAGHTILAGSSPAL
                     IDRATTVLADLGVRVRTNSMIAEATAGGFRLTGAELVEGGVFVWAGGLKAPDIVMGSG
                     LTVGHDGRVKVDQHLRA"
BASE COUNT          770 a         1566 c         1602 g          816 t
ORIGIN      
        1 gatcgcggcc tccaccaacc gcgatgagcc gttcccgaag gacaccgagt cacagatagc
       61 gcgattcacc gaacttgtcg cgaccgccgt ggaaagcgcc gaagcgcgag aagaacttcg
      121 ccgcatcgcg gaggagcagg ctgcgttgcg acgggtagcg accctcgtcg cccgcgggga
      181 agagcccgct gccgtgttcg aagcggtgac tgaggaaatc cgtgggctgt tccatgcgga
      241 cgccactgcg ctcatccgat tcgaatcgaa cggagaagcc acattcctcg gtggccgtgg
      301 atggttgcgg cagcgcttgc cgggaacccg gttccaaccg cctcacggct tcgccctcgc
      361 ctcggtccga gaggccggtc tcgtcgctca cttcggcatc gacgacgtga cgtccagaac
      421 actgctcgag gagatgcggc aggagggagt gcgctacgcg gtcgccgccc ccatcgcggt
      481 cgagggcctt gtctgggggg cgatcaacgt ctggtcgcgg cgcggaccgc ttccccccga
      541 catcgagcag cggatcgtca atttcaccga actcatcgcg acggccatcg ctaatgccga
      601 cgtgcgttcg aagctgaccc agtcgcgcgc acggatcgtg gccaccgcgg acgcaacccg
      661 acggcggatc gagcgagacc tccacgacgg agcccagcag cgactcgtct ccctggcgct
      721 cgaactccgc ctagcgctgg aatcggtgcc cgcggagctc ccagcgcttg ggaacagcat
      781 caggcgagtc gcggacgagc tggccgcggt tcaggcgcag ctccgcgaga tatcgcgcgg
      841 gatccacccc tcgatcctgt ccgagggcgg gctcgggccg gcggtgcgca cgctggctcg
      901 gcgctccccg gttccggtcg agttggacat ccagaccagc tcccggtatc cggcgtcggt
      961 ggaggtggcg gcgtacttcg tagtttccga ggcgctgacc aacacggcta agcacgccag
     1021 cggctcgcac gccgacgtgg tcgtatggga aacagacggc gccctccggc tacgtgtgtc
     1081 ggacgacggc gtcggcgggg cccagccaca ggagggcacc ggtctgaccg ggttgcacga
     1141 ccgggtcgag gcgttgggcg ggtcgatcga ggtcaccagt ccggtcggcc acggcaccgt
     1201 actggaggtt tccctaccca ccgaccggcc ggactggtca gcgtcgacgg gccgctcgga
     1261 ggcgtcttga agtggcctct ggccccaggc cccgcaattg cagccgcgcc ggcatctgca
     1321 gccgaaacat gggacgccgt ccctcaggtg gtggaggtgc ggtagctgcc gttcctgaac
     1381 tcctcgacga gtgacgggtg ggtcgggttg ccaatcgagc tcgtcccgag ccttcgcggc
     1441 gacggtccct tgatcgagga gcacgacttc ggcgaacgcg tctcccaaac gtgctcgtgc
     1501 ctcagcgtct gatccgggca ttgccccggg cgcgccggct gccactgcgg ccgcatccgt
     1561 cagttcggca acgtgcaccg tcgacgctgt tgtccccctc gagcatgatc aagtttccgc
     1621 tgtggtcgcg cggcgaaccg aggaggatcc cgggaatacc gcccgccgcc gtcgccgtag
     1681 acccgtatag gcggtaccgg acacgatgac gaccccgcgc atctctccct cggtgagcac
     1741 gccgcgctca accggttcct tccatgccac aaacgccggc ggattgagtg tggactcctc
     1801 gctgacggca agattgttcc cgtagatcca cagaccgctg atgtggatgt gggattgccg
     1861 gtgccggcga aggcgtcgac gtcgccacgt tgagagtcgc gttcaacgac atcatggcgc
     1921 gcatcccact cgccatcgcc gcatccgctg gcgaaacctg ggccagcaga accaagtagg
     1981 cagcaagttc cgggacggcc gactggcagg taggacaact ggccagatgt tcgacctccc
     2041 gacgctcagc aggagacagc gtgctccagc acgtacgcgg catcccaccg ggcgaatttg
     2101 tcggggattg cgttcacggc cgggtcactc ctttctcctg tagtgccagt ctgagggttc
     2161 tgagtccata gtggagccgg gatttgacgg taccttctgg gattcggagc cgtgctgaga
     2221 tgtcggcaat tgatctgccc tcgtagttgg cggcgttgat tacggagcgc tgctccgtgg
     2281 aaaggctcgc aagtgcctca gtgagcaacc atcggtcgag taccacgctg gtcatgtccc
     2341 cggcgctttc ctctggcgga tcctccattc gctgctcgta ccgggacgcc gcgctgcgcc
     2401 accggtcgat gatcaagttg cgtgaggctg tgtagagcca ggcacgggcc gcggcctgat
     2461 cgcgctgccc gaggctggga tccttccacg ccctgagcag cacctcttgg acgatgtcct
     2521 ccgatagctg ccgatcgtgg gtgcgccgca tcgcgaactg caatagctgg ctggcgtgct
     2581 tatcgtgcag ctcctgcagc gcagcctcgc gctgtctgct gccgacgtcc atacctccac
     2641 accgtgcctc gacgtagggc caccgtcatc gtggccagcg cccaacttgg ctgaccatct
     2701 gccggaatcc gaagattcct gctagctggg tggacaacac ctgcgcctcg aggcgccaga
     2761 tacgactctt tcgaccccga gcgagtagcg cgtaggtgct tctcggccgt ggtctccgga
     2821 cgccttcctt tcgaggttgg ggtcaagcgg ccctacagcc cagccgcacc ggtgaggcgg
     2881 gtctggaggt gctgtcggcg ctgtcccgat ccagcgagcg cagcccggtc gctggctaga
     2941 gggcttgtag agcattgaga ggacgatcaa cgacccacat cctgagcatc gctcagcgcg
     3001 accggtgacg gcacgttggt gtgaggtcga gctcaactcg cccgccacgg ccctgagtgc
     3061 ccatccaagc gcgcctggca gccgatgcgc ggcccgccga atgggggcta gccggtagtg
     3121 aaaaggtcgc gctggtcggg ggcgcagacg gaggctggcc gggtcgcctc ctacgaactt
     3181 caccggaatc gttgacaaca aagaaatgac taagggagtt gacgatggca aacatccgta
     3241 caggcgaaga aaacgcaggc cacattgatg gcgactactc ggaccgcggc gtcgtgggcc
     3301 cggccaggca gcgctcgccg atcctctggc gtgcgctcag cgcgctcctg ctgttgacga
     3361 tgggcgggat ccatctcttt ctcgtcctca ccggggtcgg cggtctgctc ggcgtgctgt
     3421 tcgtgctcaa tgcgatcgga ggggttgtgc tggcgatcgc gatgatcgcc gcgccgcgcc
     3481 ggttcctgcc tgtggcgagc gtgctcggcc tgctgttcat ggcgggcacg ttgctcgcct
     3541 tggtgctcgc gctgaccgtc gggctcttcg ggatacgtga gcagcttggt ggcgagctgg
     3601 tcccgacgac gctgatcgtc gagtcgattg gcatcatcgt gctcgcggtg acaaccggcc
     3661 tagcgttccg gatgcggtcg cggaccgttg cttccagctg agaatcggtg ctactcgagg
     3721 cggtcgatca cttgaagttg ttgccaaggc ggccagtcca ccagcgtccc cggccgggta
     3781 cgcaagatgc tcagcgttga gcctgcgcag gagtaactac gaagcagcgg gcgacgacgg
     3841 aatcgcacgc acgcgaaggc ggaggagatt catgccatga gcgtctcgga gcgcaaccac
     3901 aggattgtgg tcgcaggagc cggctatgcc gggctgcacg tcgccatgcg cctggcaacc
     3961 aagctgcgcg acaacccggg ggtccagcta acgctggtcg actggcacga cttccaccag
     4021 gtgatgaccg agctgccacg ggtcgctggc ggcacgcggg ctgcggacgc cgtccgcatc
     4081 ccactggacg acgtcgtcgc tcggggggtc cgcttcatgg aaacccaaat caccggattt
     4141 gatctcgccg gccagcagct gaagacccag tctggaccca ttggctacac ccgcctggtg
     4201 ctggcgcttg gcagccggcc caacgacttc gccatccccg gcctggcaga acgcgccatc
     4261 tcgctgtact cggttgccga cgccgagcag gtgtgggcgg cggtcaacca agctgtcgcc
     4321 acggccgcga ccgccacaga tcccgaccag cagcggcgcc tggcgaccgt ggtcatcggt
     4381 gggggcggcg ccaccggggt ggagctggcc ggcgagctgg ccgagatgct gccagagctg
     4441 gcccgcgccc atcgcctggc gcccgaccgc ccagccgtga tcctggtcga ggctggccat
     4501 accatcctgg ccggctcctc ccctgcgctg atcgacagag ccaccacggt actggccgat
     4561 ctgggtgtcc gggtacgtac caactcgatg atcgccgagg cgaccgcagg gggcttccgg
     4621 ctcaccggtg ccgagctggt tgagggtggg gtgtttgtgt gggccggcgg gctgaaggcg
     4681 ccggacatcg tgatggggtc cggactgacc gtcggccacg acggccgggt caaggttgac
     4741 cagcatctgc gcgc
//
LOCUS       DASRJQ010000376         2189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1721764, whole genome shotgun sequence.
ACCESSION   DASRJQ010000376 DASRJQ010000000
VERSION     DASRJQ010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1721764"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..821)
                     /locus_tag="VFB89_08400"
     CDS             complement(90..821)
                     /locus_tag="VFB89_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZM27364.1"
                     /translation="MAQILGIVTDATLTKLAGVRVEIVDGPSAGAFAISDLNGRVSFV
                     GAFTGVLTFRAAREGYLSATQTLNINALCSTCQAQIVFEMLPADVIAILGLGAYSLTV
                     VADSSCTGLPAELRTRSYSATITRSSFRGWYDVRVPGMLFEHGWFLIRIDGNDLVTDD
                     DSYPTLFEPLSQFAYLGIDFAFRQVFEARESMITVRFPGTFEYCVLASGSIDIGFCER
                     VPASNLLSHERCDAEHHQMIFVRRE"
     gene            complement(1206..>2189)
                     /locus_tag="VFB89_08405"
     CDS             complement(1206..>2189)
                     /locus_tag="VFB89_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27365.1"
                     /translation="AWMSANYIATDPEALSPFDRSAGNGIRCVRNLGPLPAEITRPIK
                     PLDRDFAKYTPASDATLRGYQAMYAYRKGDLDARVEGVVRDAADWREEKITFNPAYGG
                     ERMAAYLFLPKKVRPPYQTVVFFPSARVDGIENSKDLGDTEFFDYVVKSGRAVLYPIY
                     KDTYERRTDNSLPGAEQQIDLTIQRYRDVARSLDYLGTRADIDTTRLAYLGVSMGAAE
                     GVIYATLAQDRLKAVVFLDGGYFLGKAPAGGDQADFAPRLKKPLLMVNGRYDFTFSYE
                     RAQLPLFRMVGTPEADKRHAVLPTPHDVRADRAELVKEVLGWLDKYLGRVH"
BASE COUNT          425 a          705 c          661 g          398 t
ORIGIN      
        1 gtgtggtcga ctgcagcgcc tagttagacc gcgtacgacg cattcgcagc gaattgacgc
       61 cattcgggcg cccatcctgc tttccggggt cactcccgtc ggacgaatat catctggtgg
      121 tgttccgcat cacatcgctc gtgtgacagc agattgcttg cgggaacacg ctcgcaaaac
      181 ccgatatcga ttgacccgct cgccaagaca cagtactcga aggtaccggg gaatcggact
      241 gtaatcatgg actctcttgc ctcgaaaact tgccggaagg cgaaatcgat cccgaggtag
      301 gcgaactgag acaggggctc aaacaacgtc gggtagctat cgtcatcggt cacgagatcg
      361 ttgccatcta tccggatcaa aaaccaaccg tgctcgaaga gcatgccggg cacgcgtacg
      421 tcgtaccaac ctcgaaacga ggagcgagtg atcgtcgcag aataacttcg cgtccgcagc
      481 tctgccggaa gaccagtgca gctggagtca gcaacgacgg tcaggctgta cgcgccgagc
      541 ccaaggatgg cgattacgtc ggccggcagc atttcaaaca caatctgtgc ctgacacgta
      601 gaacacagag cgttgatgtt caaggtctgg gtggctgaga gatacccctc tcgagctgcg
      661 cggaatgtaa ggactccggt gaaggctccg acaaacgaca cccgaccgtt gaggtcgctg
      721 attgcaaacg ccccggcgct ggggccgtcc acaatctcca cccgaactcc ggccaacttt
      781 gtgagagtcg cgtcggtaac gatgccaagg atttgagcca cgactcgcgg agcaggctgg
      841 acgtgtgtcg gcccagtcgg gcttctgcac cctgcagcga caagtgcggg gaaggctgca
      901 aacaacgctg cggcgcgtct aggtatcatt actgctctgt gcttcatggg acgccgaacg
      961 acctgcgctt ctgctgcggg ggcctgccga agccgcagcg gtacctacgc ttaagataac
     1021 ccgcggcggc tgaggcgttt aggaccccgg cagcagcaac ctccgtggcg gcccaatcga
     1081 gagcagctgc ctgatcgcac cctcggtagc cgccttgtcg acggctggac gagccgacga
     1141 acaggcaccg gcgagcacgt agattaccaa cgacgcaagc aataacacta ctccacgttc
     1201 gagttctagt ggacccgtcc cagatacttg tccagccagc cgagtacctc ctttaccagc
     1261 tccgcccggt ccgccctcac gtcgtgcggt gttgggagca ccgcgtgccg cttgtccgcc
     1321 tccggcgtgc ccaccatccg gaagagcggc agttgcgcgc gctcgtagga gaacgtgaag
     1381 tcgtaccggc cgttcaccat gagcagcggc ttcttgagtc gaggcgcgaa gtcggcctgg
     1441 tcacccccgg cgggggcctt gccgaggaaa tagccgccgt ccaggaacac caccgccttg
     1501 agccggtcct gcgccagcgt tgcgtagatc accccttccg ccgcacccat gctgaccccc
     1561 aggtacgcca gcctggtcgt gtcgatgtcc gcccgcgtcc cgagatagtc cagcgagcgt
     1621 gccacgtccc ggtaacgctg aatcgtgagg tcgatctgct gctccgcgcc cggtagcgag
     1681 ttgtcggtcc gccgctcgta ggtgtccttg tagatgggat acagcaccgc ccgtccgctc
     1741 ttgacgacgt agtcgaagaa ctcggtgtcg cccaggtcct tgctgttctc gatgccgtcc
     1801 acgcgggcgc tcggaaagaa caccaccgtt tggtaaggcg gacgcacctt cttcggcagg
     1861 aagaggtagg ccgccatccg ttcgccgcca tacgccggat taaaggtgat cttctcctcc
     1921 cgccaatccg ccgcgtcccg gaccacgccc tccacccggg catcgaggtc ccccttccgg
     1981 taggcgtaca tggcctggta gccacggaga gtcgcgtcgg acgccggcgt gtacttcgca
     2041 aaatcgcgat cgaggggctt gatcggcctg gtgatttcgg ccggtagtgg tcccaggttg
     2101 cgcacgcagc gaatcccgtt gccagctgac cggtcgaacg gcgacagcgc ctccggatcc
     2161 gtcgcgatgt agttggcgga catccaggc
//
LOCUS       DASRJQ010000377         3665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3905504, whole genome shotgun sequence.
ACCESSION   DASRJQ010000377 DASRJQ010000000
VERSION     DASRJQ010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3905504"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..529)
                     /locus_tag="VFB89_08410"
     CDS             complement(56..529)
                     /locus_tag="VFB89_08410"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZM27366.1"
                     /translation="MRNSIPLLAAAVLLLPLSLVTPQADDTNVRYIAHDQVQAAFAKG
                     MPMVEVGDYKIHASRRDGPGAVEVHTRDTDIAYVLQGSATLVTGGVAVGLKPTGPEEL
                     RGSAIQGGETRQLAVGDVVVIPNGTPHWFKEVKAPFLYYVVKVRQAARTQTAGAM"
     gene            complement(543..1037)
                     /locus_tag="VFB89_08415"
     CDS             complement(543..1037)
                     /locus_tag="VFB89_08415"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HZM27367.1"
                     /translation="MKPAIPLLIVVALVASAGRARAQSTESKKTLTLEGAKAVAAAAA
                     AEAKRGNEGGSIAIVDDGGNLMYLERIQPTFPMGATISTEKARTAALFQKPTKVLEDA
                     IVGGRTPLLNVWSAPLNGGEPIVVDGQVVGAIGVSGASSAARDAAIALAGAAALGSKN
                     SATR"
     gene            complement(1372..2559)
                     /locus_tag="VFB89_08420"
     CDS             complement(1372..2559)
                     /locus_tag="VFB89_08420"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HZM27368.1"
                     /translation="MLSERDVAVLSSMNWERADQSLGQGGTYMKAIAVFPGKSNSVHL
                     ASLPKPSLAEIPNGRGVLIKVLRVGVDGTDKEINAAEYGAPPPGYDFLIIGHESFGRV
                     EAVGPNVTEFRPGDYVVATVRRPGHSIYDQIGTYDMTTDDVYYERGINLRHGYLTEYY
                     VDDPEYIVHIPDGLRDVAVLLEPLTVVEKGIAQAYETQRRLRVWRPRKAAVIGAGTVG
                     LLATLVLRLRGLEVTTFARTKKPSRNAELLESTGARYVSIQEESFLDAAKRVGPFDLI
                     LEASGQSASVFDGMQALAKNGVLVLTSVTGGDKQLSVPADKINLEFVLGNKAMVGSVN
                     ANREYFEMGVKDMAQAEAQFSGWLAQLLTHPVKGLENYAELFDKLTNAKGAIKIYCEV
                     AEG"
     gene            complement(2571..>3665)
                     /locus_tag="VFB89_08425"
     CDS             complement(2571..>3665)
                     /locus_tag="VFB89_08425"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZM27369.1"
                     /translation="RVARGWIERGGLALSALALVTSGSSAAAQYTQPWHGKGTTSAQA
                     VTASRVPIGVRAVASVGLTVSDLDKSLAFYTGVLTFQKVKETQRSGPAFEKLVGVSGA
                     RARVATLRLGDEYLELTQYLAPRGRPAPAAWHSNDRWFQHVAVIVSDMDRAYAQLRQH
                     DVRPASANGPQRLPAWNPNAGGIRAFYFRDPDDHPLEILWFPPGKGRAKWHQASDRLF
                     LGIDHTAIVVRDTDQSLKFYRDALGFEIAGTSDNYGPEQENLNNVPGAHLRITALRAA
                     DGPGIEFLQYLAPTTGRPYPRDEQANDLVHWQTRLLVPDAGLAASRLEAEGAPFVSRG
                     VVAVADTAAGESLGLVVRDPDGHVMHLFEP"
BASE COUNT          611 a         1188 c         1212 g          654 t
ORIGIN      
        1 agtgttccca gcgccagccg gattgccggc gtgaggacgg ggcatcgggg gctcatcaca
       61 tcgctccggc agtttgggtg cgtgcggcct gacgcacttt gacgacatag tagaggaacg
      121 gagccttgac ctctttgaac cagtgcggcg tgccgttcgg aatcacgacc acatccccaa
      181 cggcaagctg gcgggtttcc ccaccctgta tggcgctccc acgaagctcc tcgggaccgg
      241 tcggtttcaa tccaacggca acgccaccgg taaccagcgt cgcggaaccc tgcaggacgt
      301 aagcgatgtc ggtgtccctc gtgtggacct cgacagcgcc aggaccgtcg cgccggctcg
      361 cgtgaatttt gtagtcgccc acctctacca tcggcattcc cttggcgaac gccgcctgga
      421 cctgatcgtg cgcgatgtac ctgacgtttg tgtcgtcagc ctgtggggtc acaaggctca
      481 acggcagcag caggacggcc gccgccaaga gagggatcga gttccgcatc gttcgctccg
      541 ggttacctgg tcgcgctgtt cttggaaccg agagcggcag cccccgccag ggcgatcgcc
      601 gcatcccggg cggcgctgct ggcgccgctg acaccgatag cgccgacgac ctgaccatcc
      661 accacgatcg gctccccgcc gttcagtggc gcggaccaga cattgagcag cggggtccgg
      721 ccgccgacga tggcatcctc gaggaccttg gtcggcttct ggaagagagc ggcggtgcgg
      781 gccttctcgg tcgagatggt ggcacccatg gggaacgtgg gctggatccg ctcgaggtac
      841 atgaggttcc cgccatcgtc cacgatggcg atagagccgc cctcgttgcc tcgcttggcc
      901 tccgcggcgg ccgccgcggc gacggccttg gcgccttcga gggtgagagt cttcttgctt
      961 tcggtgcttt gtgcccgggc tcgaccggcg ctggccacca gggctacgac aatcaggaga
     1021 ggtatcgcgg gcttcattgc tggcactcct ccgcatgggt cggcagcagc atgacgcacg
     1081 gtgtaatgcc atcggccacg cgttggttcc cgacaaacgg cgggatgctc atgcggatcg
     1141 agtgaggaga ttacgacgtg cggcgggaac gggagaacgg gaaagcaagg gccggttgcg
     1201 ggaagtggag cgcgggtgcc cgcgtcggct cggcgtagcc gggcggtggg ccgggcgctg
     1261 gtcctaggct gcggaccacc acatcagcga gcacaggggg aatcgcggca cgacctgtgc
     1321 tcgagccggt atgcgtcacc gtgagcggcc tgcaggcgct cccggagata gtcagccctc
     1381 ggctacctcg cagtagatct tgatcgcccc tttcgcgttg gtgagcttgt cgaacagctc
     1441 ggcgtagttc tccaatccct tgaccggatg ggtgaggagc tgcgccagcc acccggagaa
     1501 ctgcgcctcc gcctgggcca tatccttgac acccatctcg aagtactcgc gattggcatt
     1561 gaccgagcca accatggcct tgttgcccag aacgaattcg agattgatct tgtcggctgg
     1621 aacgctgagc tgcttgtctc caccggtcac gctggtgagc acgagcacgc cgttcttggc
     1681 gagcgcctgc ataccgtcga agaccgatgc cgactggccc gacgcctcga ggatgaggtc
     1741 gaacggtccc acccgcttgg ccgcgtccag gaaggactcc tcctgaatcg acacataccg
     1801 ggccccagtg ctctcgagca gctccgcatt gcgggatggc ttcttggtcc gtgcaaaggt
     1861 ggtgacctcg aggcctcgaa gccgtagcac cagcgtggcc agcaggccga cggttccggc
     1921 gccgatcact gccgccttgc gcggccgcca cacccggagg cgtcgctggg tctcgtatgc
     1981 ctgggcaatc cccttctcca ccacagtgag cggctcgagg agtacggcca cgtcgcggag
     2041 gccgtcgggg atatggacga tgtattcggg atcgtccacg tagtactcgg tgagataccc
     2101 gtggcggagg ttgatgccgc gctcgtagta gacatcgtcc gtggtcatgt cgtaggtgcc
     2161 gatctggtcg tagatactgt gaccaggccg acgcacggtg gccacgacgt agtcgcccgg
     2221 acgaaactca gtcacgttgg gtcccacagc ttctacccgt ccgaagctct cgtgcccgat
     2281 gatcaggaag tcatatcccg ggggcggtgc cccgtactcc gcggcgttga tctccttgtc
     2341 cgttccgtcc acgccgacac gcagcacctt gatcagtacc ccccggccgt tgggaatctc
     2401 cgccagagag ggcttgggca gcgacgccag atggaccgaa ttggacttac cggggaagac
     2461 agcgatcgcc ttcatgtacg tgcccccttg tcccagcgat tgatccgccc gctcccaatt
     2521 catactcgat agcaccgcga cgtcgcgctc gctcagcata ttccactcct tcagggttcg
     2581 aacaggtgca tgacgtggcc gtcggggtcg cgcacgacca ggcccagcga ttcgccagcg
     2641 gcggtgtctg ccacagcgac gacaccgcga gagacgaagg gggcaccttc cgcttcgagc
     2701 cggctggcgg ctagaccggc gtccggtacc agcaggcggg tttgccagtg gaccagatcg
     2761 ttggcctgct cgtcgcgcgg gtacggtcgg ccggtggtgg gcgcgaggta ctgcaggaac
     2821 tcgatgccag ggccatcggc cgcacgcagg gcagtgattc ggagatgggc cccaggcacg
     2881 ttgttgagat tctcctgctc cggcccgtag ttgtcgctcg tgcccgcaat ctcgaagccg
     2941 agtgcgtccc gatagaactt gaggctctga tcggtgtcgc gaacgacgat cgccgtgtgg
     3001 tcgatgccca ggaaaagccg atcgctcgcc tggtgccact tcgcccgccc tttcccggga
     3061 gggaaccata gaatctcgag cggatgatca tcgggatcgc gaaagtagaa ggcgcgaatg
     3121 ccgcctgcat tcgggttcca ggcgggaagc cgctgcggcc cgttggctga agccggcctg
     3181 acgtcgtgct gccgcagctg agcgtaagcc cggtccatgt cgcttacgat cacggctacg
     3241 tgctggaacc agcgatcgtt gctgtgccag gccgccggag ccgggcgacc ccggggcgcg
     3301 agatactgag tgagctccag atactcgtcg cccaggcgca gcgtggccac ccttgcccgg
     3361 gcgccggata ccccaaccag cttttcgaac gctgggcccg agcgctgcgt ctccttcacc
     3421 ttctggaacg tgagcacgcc agtgtagaac gcgagcgact tgtcgagatc ggagactgtg
     3481 aggccgaccg acgccacggc gcgcacgccg atcgggactc gactggccgt gactgcctgg
     3541 gccgaggtgg tgcctttgcc gtgccaaggc tgggtgtatt gagccgccgc cgaactgccg
     3601 ctggtgacca gcgccagcgc cgagagggcg agtcccccgc gctcgatcca gcccctggca
     3661 acccg
//
LOCUS       DASRJQ010000378         3119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3369550, whole genome shotgun sequence.
ACCESSION   DASRJQ010000378 DASRJQ010000000
VERSION     DASRJQ010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3369550"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VFB89_08430"
     CDS             complement(<1..247)
                     /locus_tag="VFB89_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27370.1"
                     /translation="MAPKLPPGWHPARLTESDQRCRVVTVATARQPDPILLLNMGQIT
                     RLQISRAPPPDWWTEPEESEGWSEIELSRLRDETDRCR"
     gene            complement(376..642)
                     /locus_tag="VFB89_08435"
     CDS             complement(376..642)
                     /locus_tag="VFB89_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27371.1"
                     /translation="MSHTLRLDDQTVRSAGGFEPPAQPPSHVQPPLVLVQPRWQYKHL
                     VRLPVEEPGAEEAELNRLGAAGWELVNTLFDGRMVHLYLKREVQ"
     gene            complement(721..1230)
                     /locus_tag="VFB89_08440"
     CDS             complement(721..1230)
                     /locus_tag="VFB89_08440"
                     /inference="COORDINATES: protein motif:HMM:NF014037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HZM27372.1"
                     /translation="MIFFACLAALASTPRFLADAMLGRLARWLRVLGMDTAYDPDVPD
                     PMLVRWAQAEQRILLTRDRNLVAELRPQRSLLVEADAPLIQLRQVVAQVGVAPPVELF
                     TRCLVCNAELAPPDTEDIDTRIPPASRQLPGPVRYCLSCNRVYWPGSHTRRMRAALAR
                     TFPDWPLQR"
     gene            complement(1253..1825)
                     /locus_tag="VFB89_08445"
     CDS             complement(1253..1825)
                     /locus_tag="VFB89_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27373.1"
                     /translation="MAATLAHLLAGPAWAQATAPAGPAEPYIDRGVCPFECCTYREWH
                     AVNTIGVHASPQPGAAIVARLSPAAQVDALTGEVHFRQVGVVLLQRSVTLHDENAGDS
                     LALRSGDRAYVLSSLGEEYYRVWVRGRILEVPFFWDDRATYPRPTGEPGRLEQVPEEI
                     WWVQVRTPTGISGWIRMDQARVRGADQCGD"
     gene            complement(1950..2615)
                     /locus_tag="VFB89_08450"
     CDS             complement(1950..2615)
                     /locus_tag="VFB89_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27374.1"
                     /translation="MFVGHLALALAAKRAAPRVNLGWLMAGVTALDLVWPLFVLAGIE
                     HVRIVPGATAFTPLAFDSYPWSHSLVMAFVWGLVLVGLARSFGVPGSASLLLVGLVVS
                     HWVLDFISHAPDMPLWPGPSPRLGLGLWNSIPMTFVVEGSLWVAGILAYLLGHRFGGR
                     GSRTAFWSLVAVTTVMWASGPWSPPPPSVRALAWFALIGWIIVPWAVAADRGGRIDGS
                     RPD"
     gene            complement(2630..>3119)
                     /locus_tag="VFB89_08455"
     CDS             complement(2630..>3119)
                     /locus_tag="VFB89_08455"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZM27375.1"
                     /translation="GLRLPDHTPPNDPTSPMKLDIYLNYRGNCEQAFLFYEQHLGGKI
                     TSMMRHGEVPDQGNVPADWKNAILHARIEIGNTVVMGADIPAAEPMRSAYLTLRVDTD
                     EEAERLYALLSDGGQIFMKMEETFFASRFAMVRDRFGTSWMLLHERQAPQPPTSTRGT
                     AA"
BASE COUNT          589 a          987 c         1052 g          491 t
ORIGIN      
        1 gccggcagcg gtcagtctcg tcccggaggc ggctcagctc gatctcgctc cagccttcgc
       61 tttcctccgg ctcggtccac caatcgggcg gcggggcccg actgatctgg agccgggtga
      121 tctgccccat gttgagcagc aggatcgggt ccggctgccg agcggtggcc actgttacga
      181 cgcggcagcg ctggtcggac tcggtgagcc gtgccggatg ccacccggga ggaagcttcg
      241 gtgccatgac ccgcacgcgt atgtcgagcg gaatggctcc tgcaagaacg ggccgcgggg
      301 atgatgagca agacacgagg ctcatcacag cgaagaaggc agcggcgcgc tgcgcagagt
      361 acgcgccggc gtgcgtcact gcacttcgcg cttcaggtac aggtgcacca tccgcccgtc
      421 gaagagcgta ttcaccaatt cccatcccgc tgcgcccagg cggttgagct cagcctcttc
      481 agcgccaggc tcctccacgg gcaaccgcac caaatgcttg tactgccatc ggggttgaac
      541 gaggacgagc gggggctgaa cgtggctcgg cggctgggcc gggggctcga agcccccagc
      601 ggaccggacg gtctggtcat caagacgaag ggtgtggctc ataggccagc ctccctctgc
      661 agttgctctg ctttctgcgg tcacggggaa tttagagagt tggcccggcg acggttgccg
      721 tcagcgttgc agcggccagt ccgggaaagt acgcgccaac gctgcacgca tgcgcctggt
      781 gtgagacccc ggccagtaca cgcggttgca gctcaggcag tagcgcaccg ggcccggcaa
      841 ctgccgcgat gcgggcggga tacgtgtatc gatgtcctcc gtgtcgggcg gggccagctc
      901 ggcgttgcag accaggcacc gagtgaacag ctcgacgggt ggcgcgacac cgacctgagc
      961 cacgacctgt ctgagttgaa tcagtggagc gtccgcttcc accagaaggg accgctgcgg
     1021 tcggagctcg gcgacgagat tccggtcgcg agtgagaagt atgcgctgct cggcctgtgc
     1081 ccagcgcacc agcatgggat ccggaacatc gggatcgtag gcggtgtcca tgccgagcac
     1141 gcggagccag cgcgccaagc gtcccaacat ggcatcggcg aggaaccggg gtgtactcgc
     1201 cagcgccgca aggcatgcga agaagatcaa ggtacgcggc agtgtgctct cattagtcgc
     1261 cacattgatc cgcaccgcgc acccgcgctt gatccatgcg aatccagccg ctgatgccgg
     1321 tgggagtgcg cacctgtacc caccagatct cttccggcac ctgctccagg cggcctggct
     1381 cgccggtggg ccgaggatag gtcgcccggt cgtcccagaa gaagggcacc tcgaggatgc
     1441 ggccccgaac ccagacccga tagtactcct cgcccagcga gctcaacaca taggcgcggt
     1501 cgccggatct gagcgcgaga ctgtcccctg cattctcgtc gtgtagcgtc accgaacgct
     1561 gcagcaggac gacgcctacc tggcggaaat ggacctcgcc cgtgagcgca tcgacttgcg
     1621 cagcgggcga caagcgtgca acgatcgccg ctcccggctg cgggcttgcg tgcacgccga
     1681 tcgtgttcac ggcgtgccac tcacggtagg tacagcactc gaacggacag acgccgcggt
     1741 cgatgtacgg ctcagccggt cccgctgggg ccgttgcctg cgcccaggcc ggcccggcaa
     1801 gcaggtgagc cagggtggcg gccatgagga acctgtggga gtgagcctgc acactcacag
     1861 attcagccgg tatgcgttcg gaccgcgtca cttccgcggg tgagcttcga gtagtaatgg
     1921 atccgtcgag ccgcctgatg tcttgctcct cagtcaggtc gtgagccgtc gatgcgccct
     1981 ccccgatcag ctgccactgc ccacgggaca atgatccagc cgatcagggc gaaccaggcg
     2041 agagccctca cgctaggtgg aggcggagac caggggcctg atgcccacat cacggtggtc
     2101 actgcgacca gagaccagaa tgccgtgcgt gagccccgcc caccaaagcg atggcccagc
     2161 aggtatgcga gaatgccggc cacccaaagc gagccctcga ccacgaaggt cataggaatg
     2221 gagttccaaa ggcccagacc gagacgcggc gaggggccag gccacagggg catgtccggg
     2281 gcgtggctga tgaagtcaag cacccagtgg ctgacgacca agcctaccag aagcagcgat
     2341 gcggaacccg gcaccccgaa cgagcgggcg agaccgacga gcaccagtcc ccacacgaat
     2401 gccatgacga ggctgtgcga ccaggggtag gagtcgaacg ccagaggcgt aaaggcagtc
     2461 gcgcccggca cgatgcgaac gtgctcgata ccggcaagga caaaaagggg ccacaccagg
     2521 tccagggccg tgacgccggc catgagccag ccaaggttga cgcgaggtgc ggcgcgcttg
     2581 gcggccagag caagtgcgag gtggccgacg aacacagtgc gtcacggcct catgcggcgg
     2641 tacctcgcgt cgacgtaggc ggctgtggtg cctggcgttc gtgaagaagc atccaggacg
     2701 tgccgaacct gtcgcggacc atcgcaaagc gggaagcgaa aaaggtctct tccatcttca
     2761 tgaagatctg accgccgtcg gacagcaggg cgtacaggcg ctcggcttcc tcgtcggtgt
     2821 caaccctcag ggtgagatat gcgctccgca tgggctcggc cgcgggaatg tcggctccca
     2881 tgaccactgt gttgccaatc tcgatgcggg cgtgcaggat cgcgttcttc cagtcagccg
     2941 ggacgttgcc ctgatcaggc acttcgccgt gcctcatcat gctggtgatc ttgccgccga
     3001 ggtgctgctc gtagaagagg aaggcctgct cgcagtttcc gcggtagttg aggtagatgt
     3061 cgagtttcat cggactagtt ggatcattag gaggcgtatg gtcaggtaag cgcaaccca
//
LOCUS       DASRJQ010000379         4580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1237717, whole genome shotgun sequence.
ACCESSION   DASRJQ010000379 DASRJQ010000000
VERSION     DASRJQ010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237717"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VFB89_08460"
     CDS             <1..273
                     /locus_tag="VFB89_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27376.1"
                     /translation="PFSAIKPPFVSGFTSADGLVWLEKSRAAFDSVGSWHVVDQEGHL
                     VREIRLPGFSRILAAAPGAALAIESDSGHVRALQVSVPRLVSPGVS"
     gene            279..1385
                     /locus_tag="VFB89_08465"
     CDS             279..1385
                     /locus_tag="VFB89_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27377.1"
                     /translation="MVRSWWLLCLALPATAACTNSKPRRGALTLESKLPVTFSQLSNV
                     VELRDRRIVFADTRDRLFIRGDLKSGKLDTLGKRADTLGTGAAPSLYKLPGWVAHLAG
                     DTVALVDFAAVRTTLWTEQGEPVRAVPLPAAGGPTPVLVYDQAGFGYKADYQAVVGGG
                     EPGRSLRPDSISVLRIELGTGRVDTVAQLAAPEYGDATFGEQVQQVAKIFGPNDVFGV
                     LPDGHVWVARARENRIDWRGPGGVWIRGQPRTYPKVPVTQADKDRVIARIRQQGKGRG
                     LPDSLHLEWPFAENKPPFEAGTSDPQGQVWLQRARELDADEAIYDVYQTDGSWKEAVT
                     FPKGVSLAGFGSGGTVYGIIKEGDQKTVGRYKLQ"
     gene            complement(1422..2141)
                     /locus_tag="VFB89_08470"
     CDS             complement(1422..2141)
                     /locus_tag="VFB89_08470"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27378.1"
                     /translation="MNEMLGRLRSGAIQLWDVLPPLVAAAAILLAGYFLARQVQRWVD
                     DSRKRLDFDRVLHAGGLDQVVERSGSRLDPVRALAKLLFWLVMLVVVLLASAALGLES
                     INQMFGTMLAFLPTLIAAIVVVILGMIVGEFVRGLITALAGNLAGVPTLAKLAKGAVV
                     LVSVFLALQQVGVDQEIVTAAFTLILGAVALAAGLAFGLGNRDLAGEITRRWYEQGQR
                     RDRRKTDRKPGGPPDEPQLLD"
     gene            complement(2134..3174)
                     /locus_tag="VFB89_08475"
     CDS             complement(2134..3174)
                     /locus_tag="VFB89_08475"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZM27379.1"
                     /translation="MRALALDGVGGLERLAIRNLPDPPLRAPDDVRIRVHAAALNHLD
                     LFVADGLAGVDYQFPHVVGCDAAGVVDAVGPSVTTVAPGDRVMVNPGLSCGSCAACLD
                     AEESLCRTFRILGEHVSGTLAELVVVPEANLARVPDAMPWDRAAAFSLATLTAWRMLT
                     TRAFLRPGETVLIWGIGGGVAQAALQIVKLLGGFAIVTSGTAHKLEAALRLGADAVLD
                     HGKADVVAEVRRLTDGRGADVVVDSVGEKTWASTLRALKRGGRLVTCGATSGPRIELD
                     LRRLFWHQWSLLGSTMGSRREYWEIMRLAGQGRLWPIVDRVVPLENAIDAFSRLASGE
                     QFGKLVVEVSHE"
     gene            complement(3174..>4580)
                     /locus_tag="VFB89_08480"
     CDS             complement(3174..>4580)
                     /locus_tag="VFB89_08480"
                     /inference="COORDINATES: protein motif:HMM:NF022113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HZM27380.1"
                     /translation="STEIVAWLAGQERRAAVVVHKTVRSIVVTPRASAGPLVLPLDKK
                     HKFTIAAEAVDSTPIPEIAVNWEVGDSSIATFDRATSELTGKAIGTTRLTAKVSGFPP
                     ATWAIQVVPAAVRMDRSRLALSPGERTSLKASLAAEGGKSPQSSTQLTWKSDHPDVVT
                     VDQQGAVEARRFGGAKISASTSWGASADADVFVVGDMLAVSNRTGRLGIYQTRAVQPD
                     SLKPVLADSGSNIDPALSPDRTRIAFSSNRGSSEGNYDLYVMNADGSNIRRVLSAPGS
                     DGQPAWMPDGTRLVFTGTRNGVPQIFSISADSADAAPVALTTSVGGNQNPAVSPDGRT
                     IAFVSIRDGAPRIYRMAADGSGQARATTGTLREGCPEFFANGDLLFGVEKKKGSRDWR
                     VMRMPEGAAPVALFETDQPVVALSPSRDGERVVFVTGRDAGKGRIEYRAWLRGLALGA
                     QPIQFKLRPDEQFPTATF"
BASE COUNT          822 a         1501 c         1501 g          756 t
ORIGIN      
        1 cccttttctg cgatcaaacc tcctttcgtg tcgggcttca cctctgcgga tggattggtc
       61 tggctggaaa agagccgggc ggcgttcgac tcggtgggaa gctggcacgt ggtggatcag
      121 gaaggacatc tcgtgcgcga aatccgcttg cccggcttca gtcgtattct ggcagctgcc
      181 cccggcgcgg cgttggcgat cgaatcggac agtgggcacg tgagagcgct acaggtctca
      241 gttcctcgac tcgtttctcc cggagtatca tgatgtcgat ggtgagatcc tggtggctgc
      301 tctgcctcgc actccctgcg actgctgcct gcaccaactc gaagccaagg cgaggcgccc
      361 tcacgctgga gagcaagctg ccggtcacgt tttcccagct cagcaacgtg gtggagctgc
      421 gcgatagacg gatcgtcttc gcggacaccc gagatcggct cttcatccgt ggcgatctca
      481 agagcggcaa gctggatacg ctcggcaagc gggccgacac gctcggcaca ggcgcggccc
      541 cctcgttata caagctcccg ggctgggtcg cgcatctcgc cggcgacacg gtcgctttgg
      601 tcgactttgc agcggttcga accaccctct ggactgagca gggcgagccg gtcagggcag
      661 ttcctctccc agccgccggg ggcccgactc cggtgctggt gtacgaccag gctggcttcg
      721 gctacaaggc cgactaccag gcggtcgtgg gcggcggcga gccggggcgg agcttgagac
      781 ccgacagcat ctccgtgctg cggatcgagc ttggcacggg ccgggtcgat accgtcgccc
      841 agctcgcggc tcccgaatac ggcgacgcga cgttcgggga gcaggtgcag caggtcgcca
      901 agatcttcgg gcccaatgac gtcttcggcg tgctgcccga tggtcacgtg tgggtcgctc
      961 gcgcgcggga gaatcggatc gactggcgtg gaccgggggg ggtgtggatt cgcggccagc
     1021 cccgcacata ccccaaggtc ccggtgactc aggcggacaa ggaccgcgtg atcgcacgga
     1081 tcaggcagca agggaaaggc cgcggacttc cggacagcct gcacctcgaa tggccgttcg
     1141 ccgagaacaa gccgccgttc gaggcgggaa cgagcgatcc ccagggacaa gtctggcttc
     1201 agcgtgcccg tgagctggac gccgatgagg cgatctatga cgtgtaccag acggacggct
     1261 cgtggaagga ggcggtcacc tttcccaagg gcgtctcact ggccggattc ggcagcggcg
     1321 ggacggtcta cggaattatc aaggaggggg accagaagac ggtcgggaga tacaaactgc
     1381 agtaaaaagg gagaagggcc ttcccccttt tctgcggcag ttcagtccaa taattgcggc
     1441 tcgtccggcg gccctcccgg cttccgatcc gtcttccggc ggtcgcggcg ctggccctgc
     1501 tcgtaccatc gccgggtgat ttcccccgcc aagtcccgat tccccagtcc gaaggccaga
     1561 ccagcggcca gtgcgaccgc gccgagtatc aacgtgaagg ccgcggtcac gatctcctgg
     1621 tccacaccca cctgctgcag cgcaagaaaa accgagacca gcacaacggc gcccttagcc
     1681 agcttcgcga gcgtgggcac cccggccaag ttgcccgcca gcgccgtgat cagcccgcgc
     1741 acgaattcgc cgacgatcat gcccagaatc accacgacaa tcgcggcaat gagggtgggc
     1801 aggaacgcga gcatggtccc gaacatctgg ttgatgctct cgaggcccag tgccgcgctg
     1861 gcgagaagga ccacaaccag catcaccagc cagaagagca gcttggcaag ggcccgcacg
     1921 ggatcgagcc gcgatccact gcgctcgacg acctgatcga gcccgccggc atgaaggacc
     1981 cggtcgaaat cgaggcgctt ccgactgtcg tcgacccacc gctggacctg gcgcgcgagg
     2041 aaatagcctg cgagcagaat ggccgcggcc gcgaccagcg gtggaagaac atcccacagc
     2101 tgtatcgctc ctgatctcag ccgacccagc atttcattca tgtgacacct cgacgaccag
     2161 cttgccgaac tgctccccgc tcgcgaggcg actgaatgcg tcgatcgcgt tttccagtgg
     2221 gacgacccga tccacgatgg gccagagccg gccctggccg gcgagtcgca tgatttccca
     2281 gtattcgcgc cggcttccca tggtcgagcc gagaaggctc cattggtgcc agaagagtcg
     2341 cctcaggtcc aactcgatcc tgggtccgct ggtcgcgccg caggtcacca accgtcctcc
     2401 ccgcttgagg gcacgaagcg tcgaggccca ggtcttctcg cctacgctgt ccaccaccac
     2461 atcggccccc cgtccgtccg tcagtcggcg gacctcggcg acgacatctg ccttgccgtg
     2521 atcgaggact gcatcggcac ccagacgcag cgccgcttcg agcttatgcg ctgttccgct
     2581 ggtgacgatc gcgaatcctc caagcagctt gacgatctgc aacgcagcct gggcgactcc
     2641 gccgccgatg ccccagatga gcacagtttc gcccggccga aggaaggcgc gggtcgtgag
     2701 catgcgccac gccgtgagcg tcgcgagaga gaaggcggca gcgcgatccc agggcatcgc
     2761 gtcaggaacc cgggcgaggt tggcctcggg cacgaccacc agctcggcca acgtccccga
     2821 gacgtgctca cccaatatcc ggaacgtgcg gcacagtgat tcctctgcat cgaggcaggc
     2881 cgcgcagctc ccgcacgaga gaccagggtt caccatgact cggtcgcctg gggcgacagt
     2941 ggttaccgac ggcccgacgg cgtccaccac gccggccgcg tcgcagccga cgacgtgggg
     3001 aaactggtag tcgacgccgg ccagtccatc ggcgacgaag agatcgagat ggttgagggc
     3061 cgcggcgtgg acccggatgc gaacgtcgtc cggcgcccgc agaggaggat cgggcaggtt
     3121 acgtattgcc aaccgctcga ggccgcccac gccgtcaagg gcgagagcgc gcatcagaat
     3181 gtggcggtcg gaaactgctc gtcgggacgg agcttgaact ggattggctg ggctccaagg
     3241 gccagcccgc gcagccaggc gcggtattcg atccgaccct tacccgcatc gcggccggtg
     3301 acgaacacca cgcgctcgcc gtcgcgagac ggcgagaggg ccacgactgg ctgatcggtc
     3361 tcgaacaggg ccacgggcgc agcaccttcc ggcatccgca tcacccgcca gtcccgcgag
     3421 cccttcttct tctcgacccc gaagagcagg tcaccattcg cgaagaattc gggacatcct
     3481 tcacggagcg tgccggtcgt ggcgcgggcc tggccgcttc catccgccgc catgcgatag
     3541 atgcgcggtg caccgtcacg gatcgagaca aacgcgatgg ttcggccatc cggagaaact
     3601 gccgggttct ggtttccccc gaccgacgtc gtgagcgcca cgggcgcagc gtcggcgctg
     3661 tcggcggaga tgctgaagat ctgcggcacg ccgttgcgag tcccggtgaa gaccagcctg
     3721 gttccgtccg gcatccaggc gggttgcccg tcgcttcccg gagcactcaa gacccgccgg
     3781 atgttgctgc cgtcggcgtt cattacgtag aggtcgtaat tgccctcgct gctgccgcgg
     3841 ttcgagctga acgcaatgcg agtgcgatcc ggggaaagcg cgggatcgat gttcgagccg
     3901 ctgtccgcga ggaccggctt tagcgaatcg ggctgaacag cccgcgtttg atagatgccc
     3961 agcctccctg tccggttgga aaccgcgagc atatcgccga ccacgaaaac atcggcatcg
     4021 gcggatgcgc cccaggacgt ggaggctgag atttttgctc cgccgaagcg ccgggcttcc
     4081 accgcaccct gctggtccac cgtgaccacg tcaggatgat cggacttcca ggtcagctga
     4141 gtcgaggatt gaggggattt gcccccttcg gcagccaggc tcgctttgag actcgtgcgt
     4201 tctccaggcg acagcgccag acgagatcga tccatgcgga ccgcggccgg tacaacctgg
     4261 atagcccagg tcgcgggagg aaatccggaa accttggcgg taagcctggt ggtgccgatc
     4321 gcttttcccg tcagctcgct ggtcgcgcgg tcgaaggtgg cgatgctcga atctcccact
     4381 tcccagttga cggcaatttc cgggatcgga gtcgagtcga cggcttcagc cgcgatagtg
     4441 aacttgtgct tcttgtcgag gggaaggacg agcgggccgg cgctggcacg gggggtcacg
     4501 acgatggacc gcacggtctt gtgcactact accgcggcgc gtcgctcctg accggccagc
     4561 caggcaacga tctcagtgga
//
LOCUS       DASRJQ010000380         4004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2601454, whole genome shotgun sequence.
ACCESSION   DASRJQ010000380 DASRJQ010000000
VERSION     DASRJQ010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2601454"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VFB89_08485"
     CDS             <1..467
                     /locus_tag="VFB89_08485"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZM27381.1"
                     /translation="QEVFFFGTPADDPRFDDGRCPVWIEHGERFLYGIPGNQRRGFKV
                     ADDTRGPLIDPTTEERLPTAEGLDRARQLLARRFPALAGAPVVETRVCQYEQSPDGHF
                     ILDRHPAADNVWLAGGGSGHGFKMGPAVGELLAGLVLGTAHPEPQFSLARLS"
     gene            467..3028
                     /locus_tag="VFB89_08490"
     CDS             467..3028
                     /locus_tag="VFB89_08490"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HZM27382.1"
                     /translation="MILWALFAVTLSAFTIAPPALAQREPVLKQINEPHSYYFREMYL
                     PQLTSGPSGATWSPDGQELIYSMQGSLWRQRIGSKEAKQLTDGPDYAYQPDWSPDGHT
                     VAYVSYTGTAVDLRLLDLITGQSRSLKADGSVNLEPRWSPDGSRLAFVSTAFEGRFHI
                     FVMALSGGRAGQPVRITEDRDSKLPRYYYSVFDQYLSPTWSPEGKELIVVSNRGHVWG
                     SGTLWRMAADSGSPMREIHVEETTWGARPDWSRDGKRVVYASYLGRQWHQLWLMTADG
                     DNPLQLTYGDFDATYPRWSPDGRRIAYISNAAGNTSLWTVEVPGGQREQVVARSRTYL
                     EPTGRLRLTVIDAATGKPIPARISITAKDGQSFAPDDAWRHADDGVDHTERRFEYGYF
                     HTPGTSDLTIPEGTLSIEVTRGPEYEVAHQSVSVAAKSTRTIRVSIHRLMNLAARGWW
                     SGDLHVHMNYGGHYRSDPRRLRAMAEAEDLHVIENLIVNKEGRVPDIGYFTGRPDPVS
                     TSRLLILHDQEFHTSLWGHVGLLGLRDHVVLPGYASYAQTAAASFFPTNTEVMTQARE
                     QGAVTGYVHPGDVLADTAQPDKTLPYGIPADAALGLVDYMELVSFSEHVSTAWVWYRL
                     LNCGFHIPAGAGTDAMTNFASLRGPVGMNRVYVKLDGPLSRERFLGGLKAGRSFATNG
                     PLLELTIEGRGLGEEVRLPVGGGRLRVRGSLRSFVPVERLELVGRDGQVLRFPLAGDS
                     TRASIDTTIAVRQSGWYTLRASSTKSRHPVLDIYPFATTSPIYVSVGNEPVRSPQDAA
                     YFVRWIDRLDSLAQASTAWNNEAERNATLGKFAEARKVFSEQAGSAAASTPAGRN"
     gene            complement(3025..3801)
                     /locus_tag="VFB89_08495"
     CDS             complement(3025..3801)
                     /locus_tag="VFB89_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27383.1"
                     /translation="MSLYAVTRPVSASLGQCEITFIEREPIDVALARAQHAAYERALE
                     DLGVTIIRLPPADSLPDAVFVEDAALVLDELAVIPIMGALSRRPEVVEVARCLSSYRT
                     LHHLTAPATLDCGDIMQVEQTLYVGVTARTNRAAITQLQSIVGPVGYRVIPIVPTGCL
                     HLKSACTYLGRGVVLANPDWIDLTLLEGLEVLPVAPDEPFAANAVEVAGTLLYASDFP
                     ETQAAMEQAGFDVVPVDTAELRKAESAMTCMSLLFEAEAA"
     gene            complement(3798..>4004)
                     /locus_tag="VFB89_08500"
     CDS             complement(3798..>4004)
                     /locus_tag="VFB89_08500"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HZM27384.1"
                     /translation="RWPTLVMMGLIWPAVLFTGWLKYGELIVLRSFCPWCAISAVTIL
                     LCAAMVTLDWRRVRGVSLAGTSLA"
BASE COUNT          735 a         1244 c         1299 g          726 t
ORIGIN      
        1 gacaggaagt gtttttcttc ggaactcccg cggacgatcc cagattcgac gatggtcgct
       61 gccccgtatg gatcgagcac ggcgagcgct tcctctacgg cattcccggc aatcagcgcc
      121 gtggcttcaa ggtcgctgac gacactcggg gacctctgat cgaccccacg accgaagagc
      181 gcctgccgac tgccgaggga ctggaccgcg ctcgtcagct tctggcccgg cgctttccgg
      241 cgctggccgg ggcgccagtg gtggagaccc gggtctgcca atacgagcag agcccggatg
      301 gacacttcat tctcgaccgc catcccgcag cagacaacgt gtggcttgcc ggcggcggct
      361 ctggtcacgg attcaagatg gggcccgctg tcggcgagct gctcgccgga ctggtgctcg
      421 gtactgccca tccggagccg cagttctctt tggcccgcct ttcgtgatga ttctctgggc
      481 gctgttcgcc gtcacgctga gcgcgttcac cattgcgccg cccgcgcttg cgcagcggga
      541 gccggtgctc aaacagatca acgagccgca cagctactac ttccgcgaga tgtatctgcc
      601 gcagctcacc agcgggccga gcggtgcgac gtggtcgccc gacgggcagg agttgatcta
      661 ttcgatgcag gggtccctct ggcgccagcg cattggtagc aaagaggcga aacagctcac
      721 cgatggacca gactacgcgt atcaacctga ctggtcgccg gatggccata cggtggcgta
      781 tgtctcgtac accggaaccg cggtggacct tcgcctgctc gatttaatca cgggacaatc
      841 ccgctcgctc aaggcggatg gttcggtcaa tctcgagcct cgctggtcgc ctgatgggag
      901 ccgattggcg ttcgtctcga ccgcgttcga ggggcgattc cacatctttg tcatggcgtt
      961 gtcgggtggg agagctggcc agccggttcg catcacggag gatcgggaca gcaagctacc
     1021 ccgctactac tacagcgtct tcgatcaata cctctcgccc acctggtcgc cggagggcaa
     1081 ggagctgatc gtcgtgtcca accgcggaca cgtgtggggg tccgggaccc tgtggcggat
     1141 ggctgctgat tctggctctc ccatgcggga gattcacgtc gaggaaacga cctggggcgc
     1201 ccggccagat tggtcccgcg atggcaagcg tgtggtgtac gcgtcctatc tcgggcgcca
     1261 gtggcatcag ctctggctga tgaccgcaga cggcgataat ccccttcaac tgacctacgg
     1321 cgatttcgat gccacttatc cgcggtggtc gccggatgga cgccggatcg catacatctc
     1381 gaacgcggcc ggcaacacct cactgtggac agtggaggtg cccggcggac agcgggagca
     1441 agtagtcgcc cggtcgcgca cctacctcga gccgaccggt cgccttcggc tcacagtgat
     1501 cgatgctgct acgggcaagc cgattccggc ccggatttcc atcaccgcca aagatggaca
     1561 gagcttcgcc cctgatgacg cctggcgcca cgcggatgac ggcgtggatc atacagagcg
     1621 acgcttcgag tacggatact tccatacgcc cgggaccagc gacctcacca ttccggaggg
     1681 aacactctcc atcgaggtca ctcgcggtcc cgagtatgag gtggctcatc aatcggtcag
     1741 cgtagccgcg aagtcgacgc gaacaatccg ggtgagtatt catcggctga tgaatttggc
     1801 ggcccgcggc tggtggtcag gggatctcca tgtgcacatg aactacggcg gtcactatcg
     1861 aagcgacccg cgccggctca gagccatggc cgaggcggaa gacctgcacg tgatcgaaaa
     1921 cctgatcgtg aacaaggagg gccgggttcc cgatataggc tatttcaccg ggcggcccga
     1981 tccggtgtcc accagcagac tgctgatcct ccacgatcag gagttccata ccagcctgtg
     2041 gggtcacgtc gggttgctgg gcctgcgaga tcacgtagtc ttgccgggat acgcttccta
     2101 tgctcagaca gccgcagcca gtttctttcc taccaacacc gaggtaatga ctcaggcccg
     2161 tgagcagggc gcagtcaccg gctatgtcca tccaggagac gtgttggcgg atacggccca
     2221 gccggacaag acgctgccct acgggattcc ggccgacgca gcactcggcc tggtggatta
     2281 catggagctg gtttccttca gcgagcatgt atcgacggcg tgggtgtggt accgcctgct
     2341 gaactgcggc tttcacattc ccgccggagc gggcaccgac gccatgacca actttgcctc
     2401 cttgcggggg ccggtcggca tgaatcgcgt ctatgtgaag ctcgacgggc cgttgagtcg
     2461 cgagcgattc ctcggtgggc tcaaggcggg gcgaagcttt gcgaccaacg ggccgctgct
     2521 cgagctgacg atcgagggcc gtggcctggg agaggaggtt cgtctaccgg tcggcggcgg
     2581 gcgccttcgg gtgcgcggaa gccttcggtc gtttgtgccg gtggagcggc tcgagctggt
     2641 cggacgggac gggcaggttc tccgttttcc cctggccggg gacagcacgc gggcgagcat
     2701 agacaccacg atcgcggtcc ggcagagcgg ctggtacacc ctgcgggcgt cgtccacgaa
     2761 atcacgccat ccggtgctcg acatctatcc gtttgcgacg accagtccga tctacgtatc
     2821 ggtggggaat gagccggtac gatcgccgca ggatgctgcc tactttgtgc gctggatcga
     2881 tcggctcgat tcgctggcgc aagccagcac ggcgtggaac aacgaggcgg agcggaacgc
     2941 tacgttaggg aagtttgccg aagcgcgaaa ggtgttttcg gagcaagccg gttcggcggc
     3001 cgcctcaaca ccggccggcc gcaactaagc cgcttcagct tcgaacaaaa gactcatgca
     3061 ggtcatggcc gattccgcct tgcggagctc ggcggtgtcg accggcacta cgtcgaagcc
     3121 tgcctgttcc attgccgcct gagtctccgg aaaatcactc gcatagagca gcgttccagc
     3181 cacctccacc gcgttggcag cgaaaggctc gtccggtgcg accggaagaa cctccaggcc
     3241 ctccagcaac gtgaggtcga tccagtcagg attcgccagg accacgccgc ggcccaggta
     3301 agtgcaggcc gatttgagat gaaggcagcc ggtcggaacg atcggaatta cccggtaccc
     3361 aaccggcccg acgatgctct ggagctgggt gatggcagca cggttggttc gagccgtaac
     3421 accgacgtag agcgtctgct ccacctgcat gatgtcgccg caatccaggg tggccggtgc
     3481 tgtgagatgg tgcaatgtgc ggtagctgga taggcagcga gcaacctcga cgacctcggg
     3541 gcggcgggag agcgcgccca tgatggggat tacagcaagc tcgtcgagga ccagtgcggc
     3601 gtcttccacg aacaccgcat cgggcaggct gtcggccggc ggcagacgga tgattgtgac
     3661 cccgaggtct tccagggctc gctcgtaggc tgcgtgctga gcgcgcgcga gcgcgacgtc
     3721 gatcggctcc cgttcaatga aagtgatctc gcactggccg agggaggcgc tcacggggcg
     3781 cgtaacagcg tacaggctca tgccaggctc gtccccgcta atgaaacgcc acgcactcgt
     3841 cgccaatcca gtgttaccat cgccgcgcac aacaggattg tcaccgccga gattgcgcac
     3901 cacggacaga aggaccgcag aacgatcaat tcgccgtact tgagccaccc ggtgaacagg
     3961 acggctggcc agatgagtcc catcatgacg agcgttggcc aacg
//
LOCUS       DASRJQ010000381         2952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_4044135, whole genome shotgun sequence.
ACCESSION   DASRJQ010000381 DASRJQ010000000
VERSION     DASRJQ010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4044135"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VFB89_08505"
     CDS             complement(<1..277)
                     /locus_tag="VFB89_08505"
                     /inference="COORDINATES: protein motif:HMM:NF012608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27385.1"
                     /translation="MALAKSTGVNGRRGADVLRDAAINTSTPFSEDEREEVQAYRRPP
                     GMYLFLEHKGHIEKIFRNWPAKDVRVTRASSGGPILGPGELGAHGMGS"
     gene            813..1499
                     /locus_tag="VFB89_08510"
     CDS             813..1499
                     /locus_tag="VFB89_08510"
                     /inference="COORDINATES: protein motif:HMM:NF018604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 family protein"
                     /protein_id="HZM27386.1"
                     /translation="MRDKLLPAVLSLIAGSADVISFLGLGGLFTAHITGNLVILAAHI
                     VTGQAASIALLLAVPMFILALGATRLLAAGVERGGRSSLPFLLLLQFVLLAASFVLCV
                     LAGPRADPRATIAIVAGMLGVSALAVQNALVQISLADVPSTAVVTTNLTRLTMDLGDI
                     LLERNSPNVRAARRRAQHTWPVVLGFAVGAALGAWCFANVGLWSLAMPAALALLAFFL
                     GLRASSVRGA"
     gene            1496..2029
                     /locus_tag="VFB89_08515"
     CDS             1496..2029
                     /locus_tag="VFB89_08515"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HZM27387.1"
                     /translation="MRDLVDPSTLPGALLYAVLFFGTAAAVARVLRTFVRRLLSHADH
                     VAVDRTGVQFVTQLTQALIYLCATVLYAHLVPVLRQLGTALLTGVSVASVVIGLAAQN
                     TLGNIIAGFSILLYRPFRIGDLVQLAGPSGLATGRVESLSLGYTVIRADDGRKLIVPN
                     STMASQVTVNLNDVKLP"
     gene            2194..2685
                     /locus_tag="VFB89_08520"
     CDS             2194..2685
                     /locus_tag="VFB89_08520"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZM27388.1"
                     /translation="MSDLHSHHVNLRLVNKYRVTAEFPDVPGTPSLLFDERPPVGDGR
                     GPNAAMVLGAAIGDCLSASLMNCLIRAHLDVEALTATVVTRVARNDAGRFRISGIEVE
                     LCPRVPPGEPARLERCQAIFQDYCTVTESVREGIRVTVSFTQPAPDTGDIGAPAAATE
                     NPV"
     gene            2690..>2952
                     /locus_tag="VFB89_08525"
     CDS             2690..>2952
                     /locus_tag="VFB89_08525"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZM27389.1"
                     /translation="MAHVELILVLLSACVALRVVGERFGVPHPTLLVLGGALVALAPG
                     LPRPALDPDVIFLLFVPPLLYRASSTAPLREFRRQFWPIFQLSV"
BASE COUNT          462 a         1017 c          895 g          578 t
ORIGIN      
        1 agctgcccat cccgtgcgcc ccgagttcgc ccgggccgag aatggggccg ccggagctgg
       61 cgcgggtgac ccgcacgtct ttggccggcc agttgcggaa aattttctcg atgtgacctt
      121 tgtgctccag gaacaaatac atgcccggag ggcgacggta agcctgaacc tcctcacgct
      181 cgtcctcact gaatggggtc gaggtattga tggccgcatc ccgaagcacg tcggcgccgc
      241 gcctgccgtt tacccccgtc gatttcgcca gagccatgtt tctgccatag agcctgctgg
      301 acctgtcctg aacggctccc gggcgcatac cacatcgtcg ttccagccgg ggacctggtc
      361 cagcgcctcg cgctcttctt cggaaagcgt atacgtcccg tcccagacac acatagccac
      421 tgctcctgct cgctcgggcc tactctcatt ggctcgccag catcaggtgt cgagtcggct
      481 gaaagcgaac gtgtcgcgct gcccgctcgc cccgacgatc ctgaaagtcg ctccttctga
      541 gtgttcggag ggcaagccga gtgccagtgc taagtagctg atttacctat ccgatgaccc
      601 ggcgagcgta aaacactcac gaacatcgtg gaaacctcgc gagggggtcg cgattccact
      661 gagcaatagg gttcaagaga cccggttggc gggccgatag gttcccgccg aaccgggggg
      721 acgtccaccg ggcgcagggc tgaattcccc tcgagtccac ggttcgggaa gtgcagggtt
      781 gcggacggta atgactcaat taggccggct cgatgcgcga taagctcttg cctgcggtgc
      841 tgagcctgat cgccggaagc gcggatgtca tcagcttcct cgggcttggt ggcctgttca
      901 ctgcccacat caccgggaac ctggtgatcc tcgccgccca tatcgttact ggtcaggcag
      961 cgagcatcgc actgctgctg gcggtgccta tgttcatcct cgcccttggc gcgaccagat
     1021 tgctggcggc aggcgtcgaa cggggcgggc gctcgtccct accattcctg ctgctgctcc
     1081 agtttgtcct gctcgccgca tctttcgtcc tctgcgtcct ggcgggcccg cgcgcggacc
     1141 ccagggcaac gatcgcgatt gtcgccggga tgctgggcgt gtcagccctc gcggtgcaga
     1201 acgcgctggt gcagatctcg ctcgcggacg tgccgtccac agcggtggtc acgaccaacc
     1261 ttacccgcct taccatggac ctcggagaca ttctgctgga gcgcaattcg cctaacgtgc
     1321 gagccgcgcg ccgtcgcgcc cagcacacgt ggcccgtcgt cctgggattt gccgtcggcg
     1381 ccgccctggg agcgtggtgc tttgccaacg ttggcctctg gtcgctggca atgcctgcag
     1441 ctctcgcgtt gttggcgttt ttcctcggcc tccgggcgag ctccgtgagg ggcgcatgag
     1501 agacctcgtc gatccgtcca cgcttcccgg cgcgctgctc tacgccgtcc tcttcttcgg
     1561 gacagccgcg gcggtagccc gggtgctcag gacgttcgtc cggcgcctac tctcgcatgc
     1621 agaccacgtg gccgtggacc ggaccggcgt acagttcgtg acccagctga ctcaagcgct
     1681 catttatctc tgtgccacgg tcctgtacgc ccatttggta cccgtcctgc gtcagctggg
     1741 aaccgcgctg ctgaccggcg tgagcgtcgc atcagtcgtg atcgggctgg ctgcccagaa
     1801 cacgctgggc aacatcatcg ccggattctc aatccttctc taccggccgt tccggatcgg
     1861 agacctggtg caactcgccg gtccatctgg gctggccacc ggcagggttg agagcctgag
     1921 cctgggatac accgtcatcc gcgctgatga cggtcgcaaa ctcatcgtgc caaacagtac
     1981 gatggccagt caggtgaccg tgaacctgaa cgacgtcaag ctgccatagc tgccggccca
     2041 cgtcgcgccg ttctcgcgac tttcgcgaaa cattcgcgac tcccgcaggt cgatcgcgca
     2101 gccgcaacga cttacctgtg gcgcccggct tgcaactcgc gccggtcagc cgcaatggta
     2161 acgcgtcgcc gccagaccta agcatgggtg tccatgtcag accttcactc gcaccacgtc
     2221 aaccttcggc tcgtcaacaa gtatcgagtc acggcggaat tccccgacgt gccgggcacg
     2281 ccatccctgc tcttcgatga gcgcccaccg gtgggggatg gccgaggccc caatgccgca
     2341 atggttctcg gcgccgccat aggtgactgt ctctcggctt cgctgatgaa ttgcctgatc
     2401 cgggcccacc tcgacgtcga agccctgacc gccaccgtcg taactcgcgt cgcccgcaac
     2461 gacgccggtc gctttcgaat ctccggcatc gaagtggaat tgtgcccgag agtcccgccc
     2521 ggcgagccgg cccgcctcga acgttgccag gccatcttcc aagactactg cacggttacc
     2581 gagagcgttc gcgagggaat cagggtcacg gtgtcgttca cgcagcccgc gcctgacacc
     2641 ggcgacattg gcgctccggc agcggcaacc gagaacccgg tataggagcg tggcgcacgt
     2701 cgagctgatt ctggtgctgc tgagcgcctg cgtagccctt cgggtggtgg gggagcgctt
     2761 cggcgtgccc cacccgactc tgctcgtact cggcggcgcg ctagtcgcgc tcgcacccgg
     2821 gctcccgcga ccggcgctcg acccagacgt cattttcctg ctcttcgtcc cgccgctgct
     2881 ctaccgagcc tcgtccaccg caccgctgcg agagttcaga cgccagttct ggcccatctt
     2941 ccagctctcg gt
//
LOCUS       DASRJQ010000382         2055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_408327, whole genome shotgun sequence.
ACCESSION   DASRJQ010000382 DASRJQ010000000
VERSION     DASRJQ010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408327"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..771
                     /locus_tag="VFB89_08530"
     CDS             313..771
                     /locus_tag="VFB89_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27390.1"
                     /translation="MSDAAERAVEKAKQEVFALCKGKQWRMCIPAQLDDSDVVIMSAL
                     AALETENAELTDREARLGLRVRDLHAESVALKAEVDRLRGERDTQWRKFVALTKDEQI
                     AELEKRAATSAEAGKVIAAARRAADDKTSMSDPDLALNDYDRAQAQKDQG"
     gene            965..1249
                     /locus_tag="VFB89_08535"
     CDS             965..1249
                     /locus_tag="VFB89_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27391.1"
                     /translation="MSELARRLENAERIPCGCCRSCLGIREDAPCFQGDPNWDNLAAE
                     ALAYVKEKLSGSGLVKILERPDHLFSPQRKANAILALLDEPTPDPERREG"
     gene            1246..1434
                     /locus_tag="VFB89_08540"
     CDS             1246..1434
                     /locus_tag="VFB89_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27392.1"
                     /translation="MMDVCDQFISDPQDDLICNTCGEHLTLHTRWPRNFTLSDWLRWL
                     MAEKNPAQSPHNPSPERG"
     gene            1472..>2055
                     /locus_tag="VFB89_08545"
     CDS             1472..>2055
                     /locus_tag="VFB89_08545"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HZM27393.1"
                     /translation="MSGLVLSLFPGIGLLDMAFEEEGFCVVRGPDLLWGGDIRRFHPP
                     AGKFDGVIGGPPCQAFSRLVHIVKAKGQTPKPNLIPEYERIVRETGPRWFVMENVPEA
                     PMPEVDGYAVSVTLVRDSYVGGLTKRMRAFSFGRRGPTAPRLDTWIDWSALHEIEPER
                     AVVATSSKEGALAKSQQELRDGTSRRLRRSASTLP"
BASE COUNT          400 a          626 c          688 g          341 t
ORIGIN      
        1 tgcgtttata gacttggtgc gccggaagaa gccgcgagcg ttggggaggc ttgtgcgggc
       61 gcgctatgtc tgctgctccc tgcgcgatca tggctcgcac ttcattcttc actactttga
      121 cccgaggcca caacttgagc gcgcgggggc tggccagacg acgggagccc cgccaatgac
      181 cctcctctgc cgcacttgcc agaagcgccc gcgcctgagc tattcgaccc tctgccggac
      241 gtgccggcga gagagtcagc ggaggcctgc cgccaagccg cgtctgaagt tcggcgtcca
      301 gcgggagcca agatgagcga cgcggcggag cgggcggtag agaaggccaa gcaggaagtg
      361 ttcgcgctgt gcaagggcaa gcagtggcgc atgtgcatac ccgcgcagct tgacgacagc
      421 gacgttgtca tcatgagcgc cctcgccgcc ctcgaaacag aaaatgcgga gttgactgat
      481 cgagaggcca ggcttgggtt acgtgtccgc gatcttcatg ctgagtcggt tgccctcaag
      541 gcagaagtcg atcgcctccg gggggagcgc gatacccaat ggcgcaagtt tgtcgcgcta
      601 accaaggacg agcagatagc ggagttagag aaacgcgccg ccacgtcggc cgaggcgggc
      661 aaggtgatcg cagcagcgcg acgggccgca gacgataaga caagtatgtc ggatcccgac
      721 cttgcgttga atgactacga ccgcgcccag gctcagaagg accagggatg atctgcgagc
      781 atcccacgtg gaccgtgacg gtggtgatcc acacccatcg ccatctcccg aatatgccgg
      841 gctggtgttg tcagcgcggt gtcgagccgc tgcccccgta tccatcgggt acggtcaccg
      901 aggtcgcgca ttgcaaccat tgccaccagg aaatctggaa gcacccgcgc ccaggggacc
      961 agccatgagt gagttggcgc ggaggctgga gaacgcggag cggattccat gcggctgctg
     1021 tcggagttgc ctaggaatcc gcgaggacgc gccgtgtttc cagggcgacc caaactggga
     1081 caatctcgcc gccgaagccc tcgcctacgt caaagagaag ctgagcggca gcggactggt
     1141 aaagatcctg gagcgcccgg atcacctgtt ctcgcctcaa cggaaggcca acgcaatctt
     1201 ggccctcctc gatgagccca ctccggaccc ggagcgtagg gagggatgat ggacgtctgc
     1261 gaccagttca tttccgatcc gcaggacgac ctgatctgca acacgtgcgg cgagcacctt
     1321 acgttgcaca cgcggtggcc ccgaaacttc actctgagcg actggctccg gtggctgatg
     1381 gcggaaaaga accccgcaca atccccacac aatcccagcc cggagcgcgg gtaactatgc
     1441 ggaacctcgt gcgaccagag cgaaagggat gatgagcggc ctagtcctgt cgctgttccc
     1501 cggcatcggc ctgctggaca tggccttcga ggaagaaggc ttctgcgtcg tgcgcgggcc
     1561 tgatcttcta tggggcggcg acattcggcg cttccatccg ccggctggga agtttgacgg
     1621 cgtgatcggg gggcctccgt gtcaggcgtt ctcgcggctc gttcacatcg tcaaggcgaa
     1681 gggccagacg ccgaagccga acctgatccc cgaatacgag cgaatcgtca gggagacagg
     1741 gccgcgatgg ttcgtgatgg agaacgtgcc cgaggcgccg atgcccgagg tggacggcta
     1801 tgcggtcagc gtgacgctcg tgcgtgattc gtatgtcggc gggctgacca agcggatgcg
     1861 cgcgttcagt ttcggtaggc gcggcccaac cgcgccgcga ctggacacct ggatcgactg
     1921 gtcggcgctc catgagattg agcccgagcg tgctgtagtc gccacgtcga gcaaggaagg
     1981 cgccctcgct aagtcgcagc aagaacttcg cgacgggacg agtcggcgac tgcggcgaag
     2041 cgcctcgacg ctgcc
//
LOCUS       DASRJQ010000383         2480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1153222, whole genome shotgun sequence.
ACCESSION   DASRJQ010000383 DASRJQ010000000
VERSION     DASRJQ010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153222"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            717..1877
                     /locus_tag="VFB89_08550"
     CDS             717..1877
                     /locus_tag="VFB89_08550"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZM27394.1"
                     /translation="MSLATKIEPTAPKRLLALDGGGIRGLISIEVLAAIERQLRTQLN
                     RGPEFRLCHYFDYVAGTSTGAISATLISLGMSVDEIRDFYLAHGAAMFDKASVLRRFQ
                     YKYEDDRLAETLRDLFDRYLPVHERSADYPHITLGSAGLQTLLLLVMRNATTDSPWPI
                     SNNPKAKYNARDLENSNLHLPLWKLVRASTAAPTYFPPEEVQVGGRRFLFVDGGVTTY
                     NNPAFLVFLMATAGAYRLSWPVGPDKMLVVSIGTGSSPKADANLAAGDMNLLYQSGNI
                     PIALMYAALNEQDLLCRAFGRCLVGPVLDREVGTMMLTEAEEQREVLPKLFTYLRYNA
                     DLSATGLAELGLGDIRPEKVQKLDSIAHMKDLRRVGEAIARQVRLQDFAAFV"
     gene            complement(1952..>2480)
                     /locus_tag="VFB89_08555"
     CDS             complement(1952..>2480)
                     /locus_tag="VFB89_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZM27395.1"
                     /translation="GGGNVFAWARPGNYTVSVRCENMTDQSAVEVPLEILIYGTNFRF
                     NRADLTPAGLDSVRKAADELKKYPEVMVRLAGHADFVGTDAYNCNLSWRRVHTVHVAL
                     NRFGIPDSRFSAIEGFGEAYPVPDAQVTQEMRDLNTSTKDKGKWWDRRVDITSSPKAP
                     GMTGCAEPPGAMPKP"
BASE COUNT          416 a          844 c          779 g          441 t
ORIGIN      
        1 cggaacctgg tgtacgggct ctcgccccgg gacccggtga cgctggggtt cgcggcggcg
       61 gtgctggcgg ctgtgggagt ggcggcggcg tcactgccgg cctggcgggc gtcgcgagtg
      121 gatccggcga aagcgctccg aggcgagtag aaaagatccg cgcccgaagc ccggggccgc
      181 ttagcctgat gctatgtgtc ttcccgggtg cgtttctcga gaatatgtct acgcaaacat
      241 atgtctccag agtcataatt ccgtaaaggg gacttggggc gagtgtggac tgaagggaag
      301 tcgcccgaag cgaagtggcg cgcagggaag tgacacgcag ggaagtggca ctcaggggga
      361 cttgcggtga cttcgggcga gaggtggctc gagattccgg atggttctcc cgagccgaag
      421 ctcccaaacc cggacgcatt ctgggccagc tttcggcagc gaatgacgga acgccgcaag
      481 cgcgagcgcg agctacctcc ggcaagaagc ctggcggagt tcagacgctc ctgcgccatt
      541 tctcaggcga ctctggcggc ccggctcggc ctcgagcaag gccgattgtc gagactcgag
      601 cgactttccg atctacggct cagtgcgctg cgggcgtacg tcgcctctct gggcggcgag
      661 ctcgtcctgc tggcgcgatt gccggatcgc gatgttcagc ttaactctgt gggaccgtgt
      721 cccttgccac caagatcgag ccgactgccc cgaaacgact cctggccctc gacggcgggg
      781 ggatccgcgg cctcatttcc atcgaagtcc tcgccgccat cgagcgccag ctgcgaacgc
      841 agctgaaccg gggccccgag ttccggctct gccactactt cgattatgtc gcggggacca
      901 gcaccggggc cattagcgcc acgttgatct cactgggcat gtcggtcgac gagatccgcg
      961 acttctacct cgcgcacggc gccgccatgt tcgacaaggc cagcgtgctc cggcgtttcc
     1021 agtacaaata cgaggatgat cggctggccg agacgctccg cgacctgttc gaccgatatc
     1081 tgcccgtcca cgagcggtcc gccgactacc cgcacatcac gctcggcagc gccggcctcc
     1141 agacgctgct cctgctggtc atgcgcaacg ccaccaccga ctctccctgg ccgatctcca
     1201 acaaccccaa ggccaagtac aacgcgcgcg acctggagaa ctccaacctc catctcccgc
     1261 tctggaagct ggtccgcgcc agcaccgccg cgccgaccta ctttccgccc gaggaggtcc
     1321 aggtcggtgg ccggagattc ctcttcgtcg acggcggggt gacgacctac aacaaccccg
     1381 ccttcctcgt tttcctcatg gccaccgccg gcgcttaccg gctctcctgg ccggtggggc
     1441 ccgacaagat gctcgtggtg tcgatcggaa ccggcagcag ccccaaggcc gatgcgaacc
     1501 tcgccgcggg ggacatgaat ctgctctacc aatcgggcaa catccccatt gcgctgatgt
     1561 acgccgcgct caatgagcag gatctgctgt gccgggcgtt cgggcgctgc ctggtgggcc
     1621 cggtgctgga ccgcgaggtc gggaccatga tgctcaccga ggcggaggag cagcgcgagg
     1681 tgctgcccaa gctcttcacc tacctgcgct acaacgccga tctctccgct accggcctgg
     1741 ccgagctcgg cctcggtgac attcggccgg agaaggtgca gaaactcgac tccatcgcgc
     1801 acatgaagga tctccgccgc gtgggcgaag cgatcgcccg ccaggtgcgc ctgcaggact
     1861 tcgccgcgtt cgtctgaact aaaaacaagc gcggcacctc tcggtgccgc gccgtgagat
     1921 tgcttcggcg gctccgccgc ctcgcaatga cttatggctt cggcatcgcc cccggcggct
     1981 ccgcacatcc cgtcattccg ggagccttgg gcgacgacgt gatgtccact cgtcggtccc
     2041 accacttgcc cttgtccttc gtcgaggtat tcaggtcccg catttcctgc gtcacctggg
     2101 catccggcac cggatacgcc tcgccgaagc cttcgatggc gctgaagcgg ctgtccggaa
     2161 taccgaatcg gttgagcgcg acgtggaccg tgtgcacccg tcgccacgac agattgcagt
     2221 tgtaggcgtc ggtaccgacg aagtcggcgt gaccggccag ccgcaccatc acctccggat
     2281 acttcttgag ctcgtcagcc gctttgcgca cgctgtccag tccggccggg gtcagatcgg
     2341 cccggttgaa ccggaaattg gtgccgtaga tcaggatctc cagcggcact tcgaccgccg
     2401 actggtcggt catgttctcg caccgaaccg agacggtgta attccccggt cgcgcccagg
     2461 caaacacgtt cccgccgccc
//
LOCUS       DASRJQ010000384        11246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1517400, whole genome shotgun sequence.
ACCESSION   DASRJQ010000384 DASRJQ010000000
VERSION     DASRJQ010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 11246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11246
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517400"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            451..852
                     /locus_tag="VFB89_08560"
     CDS             451..852
                     /locus_tag="VFB89_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27396.1"
                     /translation="MPQFSGGFAMAIPPRAPELVYPPSFPFRKSFTVFNAIWYSILEF
                     DVFTSYDTWANLADIRINGNSIGKVPPRGWTQTGGELAAVSFPFQNGVLQTIGSFGRT
                     GSNVLEIVPPTPYDYLVVGNWRFHYYQILQP"
     gene            1180..3900
                     /locus_tag="VFB89_08565"
     CDS             1180..3900
                     /locus_tag="VFB89_08565"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZM27397.1"
                     /translation="MSALTGTAHLTRPLSLRLQVLGSILAVLLPSLLVMCFYYPYRQE
                     QIARDRLRDRAVEMAESVALAAGEALGRGDSAGLKDALEWGTQDPAVVYAGVFDSAGR
                     VLAAYDPLRHRPKAPVRTFASSAGEVDGWLQAKAPIRFRDRVIGAVELGLATTVLDQE
                     IFNNRVATAALGGLILALGILASLYLASRIATPVSALQRATAEISRGNYAISVLPGGS
                     EEIDALSQSFTGMAAELRATTDRLAAARDAAQAAERAKADFLATMSHEIRTPMNGVTG
                     MLGLLLDTPLDRWQKEYAELAHRSAEALLAVINDILDFSKIEAGKIELELIDFELRHT
                     IEDVVSLLGERASAKGLELGTLIHDSVPEMVRGDPGRLRQVLLNLVGNAVKFTERGEV
                     LVRVELTGENGEAVTLRFEVADTGVGIPPDVQERLFQPFTQADASTTRRYGGTGLGLA
                     ICGRLVSIMGGEIGIRSEPGRGSTFWFTIRVSRGGAVAADSRPASASLVGVRTLIVDD
                     SPSIREGLTQQLERWGMDAAAVADGDMALDALRSATAEGRPYELTLIDMHMSEKGGLE
                     LGRRIKDDSGIAPTHLVLLAAIGTRGQARAAQEAGFDAFLTKPLRQSALYDCLIAVLD
                     RSAGGTGEPASTTLITRHTLAEARLNRRPRILVADDHEINRAVTVGILERLGYRADVV
                     TNGREAVEAVGRTRYGAVLMDCQMPLMDGYEAARAIRRQEPVAHRLPIIALTADDSEA
                     SRDRCVAAGMDDFVPKPLNRDQLGNALHRWVPDSATIGHVERESEGGAGTPSTMGPQA
                     LLTLAPLASLVDGDREKLRRYLDLFASSTTTLVTQIAAASQDRDRETLGRLAHSLKGT
                     CGSVGAGEMAALADALGVAADLSDWAGVAEHCRELESCFTRTKAAAAAAA"
     gene            3994..5010
                     /locus_tag="VFB89_08570"
     CDS             3994..5010
                     /locus_tag="VFB89_08570"
                     /inference="COORDINATES: protein motif:HMM:NF019292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HZM27398.1"
                     /translation="MLNYIWAGLIVTSFVFAIGFDARDLAGDKYRNGRPLPVALTFTQ
                     AYDSSARRVPVVVRIDPQQYSAFYGTDEKPAASYSGYLVQTREGTQLRFAADAKFPEP
                     LATIAKVSKSRDQELQGRLEGFSPPSPAQAPDTISLATAGLIFEPVRFVKLNAIAAAA
                     LDFAKTAAEIALGLIGVLSLFLGLLKIADDAGIVRALVKLVRPVLRPLFPEVPADHPA
                     LGFIALNLAANFFGLGNAATPFGLKAMEELQTLNRSQDTATNSMVMLLAMNTAAVQLV
                     PPVLLLALLGLQINRLIFPIILSTVLSLIIAITAARLYGRLRVYRDSDPNRIPPAPSA
                     APQA"
     gene            5018..5560
                     /locus_tag="VFB89_08575"
     CDS             5018..5560
                     /locus_tag="VFB89_08575"
                     /inference="COORDINATES: protein motif:HMM:NF019292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HZM27399.1"
                     /translation="METIRNTINLVSYFIIPLMLVGFPVYGLYKRVPVYESFVGGAKE
                     GFQVAVRIIPYLVAILFAVAMFRASGAMDALTRLLGPILAPIGFPAEVLPMALIRPLT
                     GSGSAALVVDMINRYGEDSIYVKMASVMFGSTETTFYVIAVYFGAINVKKTRHAVPVG
                     LTADISAMLFAVWSVRLLFG"
     gene            complement(5585..6013)
                     /gene="tsaA"
                     /locus_tag="VFB89_08580"
     CDS             complement(5585..6013)
                     /gene="tsaA"
                     /locus_tag="VFB89_08580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HZM27400.1"
                     /translation="MRAQRNESHYTLHPIGVVRSALTSLETAPLQGDEGAPEAWLEVM
                     PSAAQAMVGIEAGDELFVLTWLHRAQRDVLQVHPRGRREIPLTGVFATRSPDRPNPVG
                     LHRVSVLEVSARGLRVAPIEAIDGTPVVDIKPVLGAIDER"
     gene            complement(6036..6488)
                     /locus_tag="VFB89_08585"
     CDS             complement(6036..6488)
                     /locus_tag="VFB89_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27401.1"
                     /translation="MSHAKIIAGVVGTCLLILLTGWGFRMYSVSDPWAPYTRAVRTYL
                     SAGLHGDSAALAAGSAATQPPLWVQDAIRQHPAMVAAWTRQLNAVAGFRRGDTVTIAL
                     VSAVEGCSNLSSVTAQLLNHSARPRVLALASPCTRSDLPALLPYQRRW"
     gene            complement(6561..8132)
                     /locus_tag="VFB89_08590"
     CDS             complement(6561..8132)
                     /locus_tag="VFB89_08590"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HZM27402.1"
                     /translation="MRTPLTPLEFARRTRSLYGGREAVVDGDQRWTYAQFLDRCDRWA
                     CALQRLGVAQGDRVAYVAPNTHAHLEGFYGVPALGAVMVPLNYRLLPSDWAYMVNHSG
                     SRVVCVHADYLDLVDSVRQDMPTVEHFIALEGSNKNWLHYEQLLAEATPEFAPPEIAE
                     DDLLTINYTSGTTSKPKGVMITHRNAWINAVGTLVHHPMTCADRYLWTLPMFHANGWT
                     FVWIVTAAGGTHICARKVDAPTVYGLLSSERATMLCAAPTVLINIATAPEELRATAPR
                     GVRVLTAGAPPAAHTIERVEEQLGWTITQVYGLTETSPFITVCEPRPEHAALSAADRA
                     RIKARQGVELITSGALRVVDEAGRDVPRDGATVGEIVARGNVVMAGYYRDPEATERAF
                     QGGWFHSGDAAVVHPDGFVEIRDRLKDVIISGGENISSIEVEGVLLRHPSVQEVAVVG
                     LPHHKWGESPHAFVVLRPGAALTEEELRGFARGHLAGFKVPSGATFVAELPKTATGKI
                     QKYVLRGGRAAIVAQ"
     gene            complement(8199..8396)
                     /locus_tag="VFB89_08595"
     CDS             complement(8199..8396)
                     /locus_tag="VFB89_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27403.1"
                     /translation="MPQGDDRFDCAVASYVVSQHYARSLQYAYTGLGWWGYDRFWGYS
                     RFGVGFPRRIIVYRRLPVRGQ"
     gene            8543..9907
                     /locus_tag="VFB89_08600"
     CDS             8543..9907
                     /locus_tag="VFB89_08600"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HZM27404.1"
                     /translation="MCKFLYAVGIAGAMSAAAACNSDNPTTPSATATGQDLASVAAEV
                     RLLAVNRGIIRLVKPAPVRPELVRLGQALAFDKELSGNRDISCMTCHLPKFATGDGRS
                     LSVGQGGGGLGPARTVPQGGFIPRNAPPLFNLFALKPLFLDGRVAHEIGKGYDTPAGM
                     HLTPDMTSVFEFGALSAQPLFPVLSRAEMRGDGSSNELAALDDKQEPQIWSRIMTRLG
                     RIQEYRRMFEAAYPGKRFDEMNFAYASNAIAGFLIDRLAFNNSPWDRFLAGDDNALTA
                     TQLAGAKDFMSAKCSICHTGAAFTDNKFHNVAVAQLGPGEGDGPSKHDDFGRMRVTGN
                     PAEQYAFRTSPLRNVELTGPYGHDGAFTELHGFVDHYSDSDMKLRKYLASQLEPALQS
                     TVLQDFDAILATRDPLLKGVHFPATTIDEVTEFMKALTDPAARDLHALAPARVPSGLP
                     VDGN"
     gene            complement(10020..>11246)
                     /locus_tag="VFB89_08605"
     CDS             complement(10020..>11246)
                     /locus_tag="VFB89_08605"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="catalyzes the conversion of salicylyl-CoA to
                     gentisyl-CoA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HZM27405.1"
                     /translation="AFSLLTRSLRITHENLKLRDPALMARVDRWFAEQSAKQSRCQVP
                     VDPCPSPLFTPYRLRELVLQNRIVVSSMCQYRAEDGMPNDWHLVHLGSRAIGGAGLVM
                     TEMTDVSREARISPGCAGMYLPEHVAGWKRIVDFVHSESVAKIGIQLAHAGRKGSTCV
                     LWEGEDEPLPEGNWPLIAPSPIPYRPHNQVPREMTRSDMDQVAADFVRATRMADAAGF
                     DLIELHLAHGYLLSSFISPLTNRRTDEYGGSLANRMRYPLEIVSLVRAIWPDAKPLSV
                     RISATDWAPGGLEGHDAVEIARMSIAAGADIVDVSAGQTVPEAKPVYGRQFQTPFSDR
                     VRHEARVPTMTVGNISSAADVNTILAAGRADLVVLARAHLWDPYWTRHAAFDLGYSLP
                     WPPMYATLNRYTPRMK"
BASE COUNT         2029 a         3624 c         3563 g         2030 t
ORIGIN      
        1 cccgagcgtc ctctgtcaga gggcgctcgg ctttttttgc ggcaggcaga tgaacagaac
       61 ggcaaatctc cgaagcccag aaattcggct tgcggccctt ttcagccagc cgtaccttcg
      121 ctcgactcga agtcctgcga ctcgcaacgt cgagggagga acctcatgga tctacaagca
      181 cgccaagaag aacttcaaaa gctagcaagt gaccaagggg tcgactggaa aggacctctg
      241 gatagcttgc ctgacgataa gcgtcttcgg atcgcggctc tcgccaccct tctgaacatg
      301 gatcccatgc aagccttgag agacgagggg tccatcagga agctcgcgtc cgaggcacgt
      361 gagcggcagc gtagcattga gacacacgac aagagggaaa ggaagtttct gcgcagcctg
      421 ggaatggacg tgaacgacgg aggttaaccg atgccccagt tttccggcgg gttcgcgatg
      481 gcgataccgc ctcgcgctcc ggagctcgta tatccgcctt cgtttccttt tcggaagtct
      541 ttcacggtct tcaacgcgat ctggtattcg attctggaat tcgatgtctt tacctcgtac
      601 gacacctggg ccaacctggc tgacatccgg atcaacggaa acagcatcgg gaaggtgccg
      661 cccagagggt ggactcaaac cggtggcgag cttgccgccg tctcgttccc cttccagaac
      721 ggtgtgctgc agacaatcgg aagctttggc cggacgggtt ccaacgtgct cgagatcgta
      781 ccgcccactc cgtacgacta tttggtggta ggcaactggc gattccatta ttaccagatc
      841 ctgcagccct gaccttcagt caacgatcga cctgcgtttc acaccttttt ttcctcggat
      901 ggccggcggc gattccagtg cgtcgccggc cgtcgccggc ggcatcagtc ccggtataga
      961 tcctgccaaa gactgcactt cgaccactac tccgacgcgc cgggcctagc caccgtcccg
     1021 tgtgctaact gatttgatct ggacaagtta gaccgtcgct ccaggcaaag gcctggggaa
     1081 taggcaccct tcacccgttc cggctcgagc cggcagacgc aggttcctca tgatccgtgc
     1141 ggcgctctct cgcgtaacgc cgtatggcca ccccgccaaa tgagcgcgct gacgggcacg
     1201 gcgcacctta ctcgcccgct ctccctgcga cttcaggtgc tcgggagcat cctcgcagtg
     1261 ctgctcccca gtctgctcgt gatgtgcttc tactatccct atcggcagga acagattgcg
     1321 cgcgaccggc tccgcgaccg cgcggtcgag atggcggagt cggtagctct cgcggcggga
     1381 gaggcgctcg gtagagggga ctctgccggg ctgaaggatg cactggagtg gggaactcag
     1441 gacccggccg ttgtgtacgc gggtgtattc gactcagccg gtcgggtcct ggcagcgtac
     1501 gatccgctcc gccaccgccc caaggccccg gtccggactt tcgcctcgag tgctggagaa
     1561 gtagacggat ggcttcaggc gaaagcaccg atacggtttc gcgatcgggt catcggtgcg
     1621 gtggagctcg ggctcgccac cacggtgctg gatcaggaga ttttcaacaa ccgggtcgca
     1681 accgccgcgc tcggcgggtt gatcctcgca ttgggaattc ttgcctcgct ctatcttgcc
     1741 agccgcatcg ccacaccggt atccgcactt cagcgagcca ctgcggagat cagtcgcggc
     1801 aactatgcga tttcggtgct ccccggaggc agcgaagaga tcgacgcctt gagccaatcg
     1861 tttaccggta tggcggcaga gcttcgcgcg accacagacc gccttgcagc tgctagggat
     1921 gccgcgcaag cggctgagcg tgcaaaggcc gacttcctgg ctaccatgag tcacgagatc
     1981 cgaactccca tgaacggcgt cacgggtatg ctcggtctcc tgctggacac gcctctcgac
     2041 cgttggcaga aggagtacgc cgagttagcc caccggtcag cggaagcact gctcgcggta
     2101 atcaacgaca ttctcgactt ctccaagatc gaggccggca aaatcgagct cgagctcatc
     2161 gacttcgagc tgcgccatac cattgaagat gtggtcagcc ttctggggga gcgagcctca
     2221 gccaagggac tggagctcgg cacactgatc cacgacagtg tgccggagat ggtacgcggg
     2281 gatcccggcc gactgcgtca ggtgctgctc aacctggtcg gcaatgccgt caagttcacc
     2341 gagcggggag aagtcctcgt ccgagtcgag ctcaccggcg agaacggtga ggccgtaaca
     2401 ctgcggttcg aggtcgcgga cacgggtgtc ggaatccctc cggacgtgca ggagcgcctt
     2461 ttccaaccct tcacgcaggc ggacgcttcc accacccgcc gctacggagg gaccgggttg
     2521 gggctcgcga tctgcggccg tctggtgagc atcatgggtg gtgagatcgg gatccggagc
     2581 gaaccgggcc gcggcagcac gttctggttc accatccggg tcagtcgagg cggggcggtc
     2641 gctgcggatt cgcggccggc gtcggcctcg ctggtcggcg tgcgcacgct catcgtcgat
     2701 gatagcccaa gcatacgtga gggtctcaca cagcagctcg aacggtgggg aatggatgcg
     2761 gcggccgtcg ctgacgggga catggcgctg gacgctttgc ggtcggcaac cgcagagggt
     2821 cgcccctacg agcttacgct gatcgatatg cacatgtcgg agaagggtgg gcttgaattg
     2881 ggccgacgga tcaaagacga tagcggtatc gcgccgacgc acctggtcct cctcgccgcg
     2941 attggcaccc gtggccaggc gcgcgcagcg caggaggctg gcttcgatgc cttcctgacc
     3001 aaacccctgc gccaatcggc gctgtacgat tgcctcattg ctgtgctgga tcgttcggct
     3061 gggggcaccg gtgagcctgc gtcgacgacg ctgatcacgc gacatacgct tgccgaggca
     3121 cgcttgaacc gccggccccg catccttgtc gccgacgacc acgagatcaa tcgcgccgtg
     3181 acggtcggca tcctcgagcg gctgggatac cgcgctgacg tggtgacgaa cggtcgggaa
     3241 gccgtggaag ccgtcggtcg cacccgctat ggggcggttc tcatggattg ccagatgccg
     3301 ctcatggacg gctatgaggc cgcccgcgcg atccgacggc aggagccggt ggcccaccga
     3361 ttgccgatta tcgcgctgac ggccgacgac agcgaagcaa gtcgagatcg ttgtgtggcc
     3421 gccgggatgg acgacttcgt ccccaagccg ctgaaccgcg atcagctcgg caacgccctt
     3481 caccgatggg taccggatag cgcaacgatc ggacacgtgg agagagaatc ggaagggggc
     3541 gccggaaccc catcaaccat ggggccacaa gccttgctca cgctggcgcc tctcgcgtct
     3601 ctggttgatg gcgaccggga gaaattgcgc cggtacctcg acctcttcgc atcatccaca
     3661 acgacgctcg tcacccagat cgctgccgcc agtcaggatc gcgaccggga gacactcggc
     3721 cggctggcgc acagcctcaa ggggacgtgc gggagtgtgg gtgcgggaga gatggctgcc
     3781 ctcgcagacg cactaggagt ggcggctgac ctgagcgatt gggccggtgt cgccgagcac
     3841 tgccgagaac tggagagctg tttcactcgc accaaggcag ccgcggcggc ggcagcttga
     3901 aatagagatt ggccgtacgc gcgatagact ccgcgcttcg accatacata tttctcccga
     3961 tccccacatc catccaaatt ttcaggcccc acaatgctga actacatctg ggcaggcctg
     4021 atcgtcacga gcttcgtctt tgccatcggc ttcgatgccc gcgacctcgc gggcgacaag
     4081 taccggaatg ggcgaccgct tccggtggca ttgacgttca cgcaggcgta cgactccagt
     4141 gcgcggcgcg tgccggtcgt ggtccggatc gatccacagc aatactcggc gttctatgga
     4201 acggacgaga agcctgccgc gagctattcg ggctacctgg tgcagacgcg ggaaggaacc
     4261 cagctccgct ttgcggccga tgccaaattc cccgagccgc tggccacgat cgctaaggtt
     4321 tctaagtcgc gcgaccagga gctgcagggt cgactggagg gattctcgcc gccctcgccg
     4381 gctcaggcgc cggacacgat ctcgctcgcg acggcaggcc tgattttcga gccggtccgc
     4441 ttcgtcaagt tgaatgcgat cgccgctgcc gcgctcgact ttgccaagac cgccgcggag
     4501 atcgcgctgg gcctgatcgg cgtgctgtcc ctctttctcg ggttgctcaa gatcgccgac
     4561 gatgcgggca tcgtgcgggc actggtgaag ctggtccgac cggtcctgcg ccccctcttc
     4621 cccgaggtac cggccgacca ccccgcgctc ggattcatcg cgctcaacct ggccgccaat
     4681 ttcttcgggc tgggcaacgc ggcgacgccg ttcggattga aagccatgga ggagttgcag
     4741 acgctcaacc gctcgcagga cacggccacc aactccatgg tcatgctcct ggcgatgaat
     4801 acggcagccg tgcagctcgt gccgccggtg ctactccttg ccttgctcgg gcttcagatc
     4861 aaccggctca tcttcccgat cattctgtcc accgtgctat cgctgatcat cgcgatcaca
     4921 gccgcacggc tctatggccg gctccgggtg taccgcgact ccgaccccaa ccggattccg
     4981 ccggcgccgt cggcggcccc acaagcctga ggccggcatg gaaaccatcc ggaacaccat
     5041 caatctcgtc tcctacttca tcatcccgct gatgctggtg gggttcccgg tgtacgggct
     5101 ctacaagcgg gtgccggtgt atgagagctt cgtcggagga gccaaggagg gatttcaggt
     5161 cgcggtccgc atcatcccct atttggtggc gattctcttt gccgtggcga tgttccgtgc
     5221 cagtggagcg atggatgcgc tgacccggct cctgggcccg atcctcgcgc cgatcggctt
     5281 tcccgcggaa gtgctgccga tggccctcat tcggccgctc acgggctcgg ggtcggcggc
     5341 gctggtggtg gacatgatca atcggtatgg agaggactcg atctacgtga agatggcctc
     5401 ggtcatgttc ggctcgaccg aaaccacctt ttatgtcatc gcggtgtatt tcggggccat
     5461 caacgtcaag aagaccagac atgcagtacc ggtgggactg accgccgaca tctccgccat
     5521 gctctttgcc gtctggtcgg tgcgtctgct gttcggctga gacgtaacaa ttatcctgaa
     5581 cccatcatcg ttcatcgatt gcgccgagca cgggctttat gtcgacgacg ggggtaccgt
     5641 cgatcgcctc gatcggtgca accctgagcc cccgggcgga gacctccagt accgaaaccc
     5701 ggtgaagccc cacggggttg ggacggtccg gcgaccgtgt cgcaaacacg ccggtcaacg
     5761 ggatctcgcg tctcccccgc ggatgcacct gaagcacgtc gcgttgcgcg cgatgcagcc
     5821 acgtcagcac gaaaagctcg tcgccggcct cgattccgac cattgcttgc gctgccgaag
     5881 gcatgacctc gagccaggct tcgggggcgc cttcatctcc ctggagaggc gccgtctcga
     5941 gactggtcag ggcggagcgc accacgccga tggggtggag cgtgtagtgg ctttcgtttc
     6001 tctgcgcacg cattgcggtc atcccctcgt cttcgttacc atcggcgttg gtacggcagc
     6061 agcgccggca agtcactgcg agtgcagggc gaggcgaggg cgagaacccg cggcctggct
     6121 gagtgattga gcagttgtgc ggtcacgctg ctgaggttgg aacacccctc cacggcagag
     6181 accagcgcaa tcgtgaccgt atctcctcgc ctgaaaccag ccaccgcgtt cagttgccgg
     6241 gtccaagcgg cgaccattgc tgggtgttgc cgaatagcat cctgcaccca gagcggcggc
     6301 tgcgtagcgg cggaccctgc cgcgagagcc gcggagtcac catgaagccc agcactgaga
     6361 tacgtgcgca ccgcgcgagt gtagggcgcc caggggtctg agacagagta catccggaag
     6421 ccccagccgg tgagcagaat caggaggcag gtaccaacta cgcctgcgat gatcttcgcg
     6481 tgagacatga gatcctcctg cggcttacct gatacgccgc caccgctaca gcgaacgcca
     6541 ccgggcgcta ctgaagcaac tcactgcgcg acgatcgccg cgcgccctcc gcgcaacacg
     6601 tacttctgga tcttcccggt ggcggtcttc gggagctccg cgacgaacgt cgctccgctc
     6661 ggcaccttga agccggcgag gtggccgcgg gcaaagcctc ggagttcctc ctcagtgagc
     6721 gctgcccctg gccggagcac gacgaacgcg tggggcgact ccccccactt gtggtgcggg
     6781 agcccgacga ccgccacctc ctgaaccgag ggatggcgga gcagcacccc ctcgacctcg
     6841 atcgaagaga tgttttcgcc gccgctgatg atcacgtcct tgagccggtc ccgaatctcg
     6901 acgaaaccat ccgggtgcac caccgcggcg tcgcccgaat ggaaccagcc gccctggaac
     6961 gcacgttccg tcgcctcggg atcgcggtaa tagcccgcca tcacgacatt gcctcgcgcg
     7021 actatctcgc cgaccgtggc accatcgcgc ggcacgtccc ggcccgcttc gtccacgacc
     7081 cgcagcgcgc ccgatgtgat taactccacg ccctgtcgag ccttgatccg cgcccggtcg
     7141 gccgcagaga gcgcggcgtg ctcaggcctg ggctcgcaga cagtgatgaa gggcgatgtc
     7201 tcggtgagac catagacctg cgtgatggtc cagcccagct gctcctccac ccgctcaatc
     7261 gtgtgcgcgg cgggtggcgc accggcggtg agcacgcgca ctccccgcgg cgccgttgct
     7321 cgcagctcct cgggggcagt ggcaatgttg atgagcacgg tcggcgccgc gcagagcatt
     7381 gtcgcccgtt cgctgctgag caggccatag accgtcgggg cgtccacctt gcgcgcacaa
     7441 atgtgggttc ccccagccgc ggtgacgatc cacacaaacg tccacccatt ggcgtggaac
     7501 atcggcagcg tccacagata gcgatcggcg cacgtcatcg ggtgatgcac cagcgttccc
     7561 acggcgttga tccacgcatt gcggtgcgtg atcatcacgc ccttgggctt cgatgtggtg
     7621 ccgcttgtgt agttgatggt gagcaggtca tcctcggcta tctccggtgg ggcgaactcc
     7681 ggtgtcgcct cggcgaggag ctgctcgtag tggagccagt tcttgttgct tccctcgagt
     7741 gcgatgaagt gctcgaccgt gggcatgtcc tgcctgacgc tatcgaccag atccaggtaa
     7801 tccgcgtgca cacagacgac acggctcccg gaatggttga ccatgtaggc ccagtcggag
     7861 ggcagcagac gatagttgag cggaaccatg acggcgccga gagcggggac gccgtaaaac
     7921 ccctcgaggt gtgcgtgagt gttgggagcg acgtaggcca cgcggtcgcc ctgcgcgacg
     7981 ccgagacgct gaagggcgca ggcccagcga tcgcagcgat cgaggaattg ggcgtaggtc
     8041 caccgctggt cgccatcgac gacggcttca cggcccccgt acagggacct ggtacgacga
     8101 gcgaattcga gtggcgtgag tggagtgcgc atgtctcgag gttgagctga gcggctgcca
     8161 cgataggcta ctgcacgctc agcctcggcg tcaaggactc actggccgcg gaccggcagg
     8221 cgccgataga caatgatgcg ccgaggaaac ccgacgccga atctcgaata gccccagaac
     8281 cggtcgtagc cccaccagcc cagcccggtg tacgcatact ggaggctgcg cgcatagtgc
     8341 tgcgagacga catagctagc gacggcgcag tcgaagcgat cgtcgccttg cggcaccctg
     8401 cccccaggcc ggctgaagcc cgcggttgaa tctcgaatga ccggatctcc accggatttg
     8461 cgcttggcaa gtagggaccg atcgccgaat cgccggcgct ggccgtttgg gcctggacgg
     8521 tcccatccgg tggaggctcg tcatgtgcaa gttcttgtac gctgttggca tcgcaggcgc
     8581 gatgtccgcc gccgctgcat gcaattcgga taacccaact acaccgtccg ccactgccac
     8641 agggcaggac ctggcctctg tggcagccga ggttcggctg ctcgccgtga accgcggcat
     8701 cattcgactc gtcaagccag ctccggtacg acccgagttg gtacgtctcg ggcaggccct
     8761 cgcgttcgat aaggagttga gcggcaaccg cgacatctcg tgcatgacgt gtcaccttcc
     8821 caagttcgcg accggcgacg gtcgcagcct gtcagtcggg caaggtggcg gcggactggg
     8881 cccggcgcgg acagtgccgc agggaggttt catcccgcgg aacgcgccgc cgctgttcaa
     8941 tctgttcgcg ctcaagccgt tgttcctgga tgggcgcgtc gcccacgaga tcggcaaagg
     9001 ctacgacaca ccggcaggca tgcatctcac ccctgacatg acgtcggtct tcgaattcgg
     9061 cgccctgtcg gcgcagccgt tgtttccagt gctgtcacgc gcggagatgc gtggcgacgg
     9121 gagcagcaac gagctcgccg cactcgacga caagcaggag ccccagatct ggagtcgcat
     9181 catgacgcga ctgggccgga ttcaggagta tcgacggatg ttcgaagccg cctatccggg
     9241 caagcggttc gacgagatga atttcgccta tgcgtcgaat gcgatagcgg gattcctcat
     9301 cgatcgactc gcattcaaca actcgccgtg ggaccgcttc ctcgcgggag atgacaatgc
     9361 gctcaccgcc acgcaactgg ccggtgccaa agacttcatg agcgcgaaat gctccatctg
     9421 ccacaccggg gctgcattta cggacaacaa gtttcacaat gtggccgtgg cccagctggg
     9481 gcctggcgaa ggcgacggcc cgagcaagca cgacgacttc ggccggatgc gggtgaccgg
     9541 gaacccggcg gaacagtacg ccttccgcac gtcgccgctc cgcaacgtgg aactgacggg
     9601 accctacggg cacgatggcg cgttcaccga gctccacggg ttcgtggatc attacagtga
     9661 ctccgacatg aagttgcgga agtacctcgc gagtcagctc gagcctgcgc tgcagagcac
     9721 cgtgcttcag gacttcgacg ccatcctggc gacgcgggac ccgctactca agggcgtgca
     9781 cttcccggcg accacgatcg acgaggtgac cgagttcatg aaagcgctga cggatccggc
     9841 agcacgcgat ctgcacgcgc tggccccggc acgtgtgccg agcggattgc cagtcgatgg
     9901 gaactaagtt ggagctatca gctatcagct atcagattgc ccctagaacg gcgaccctcc
     9961 gtggacttca cggagggccg tttgctcatc ggagcggctg atagctgata gctgctagct
    10021 cacttcatcc tcggcgtata ccggttcaac gtcgcataca ttggcggcca cggaagcgag
    10081 tagccgaggt cgaatgctgc atggcgggtc cagtacggat cccaaaggtg ggcccgcgcc
    10141 agcaccacca gatccgcgcg gcccgctgcg aggatggtgt tcacgtcggc ggcggaggag
    10201 atgttcccca ccgtcatggt gggcacccgc gcttcgtgcc ggacccggtc gctgaacggt
    10261 gtctggaatt gccggccgta gaccggcttg gcttcaggaa ccgtctgccc ggccgagaca
    10321 tctacgatgt ccgcgcctgc ggcgatcgac attcgtgcga tttctacagc gtcgtgccct
    10381 tccaggcctc cgggcgccca atcggtagcc gagatcctga ccgagagcgg cttggcatcc
    10441 ggccagatcg cccgcaccag gctcacgatc tcgagcgggt accgcattcg attggccagc
    10501 gaccctccgt actcgtccgt acgccgattg gtgagcggcg aaatgaagga cgacaataga
    10561 tagccgtgag ccagatgcag ctcgatcaaa tcgaacccgg ccgcgtccgc catcctggtg
    10621 gcacggacga agtccgcggc gacctggtcc atgtcacttc gggtcatctc ccgggggacc
    10681 tgattgtgag gtcgataggg aatgggagat ggcgcgatga ggggccagtt tccttcgggg
    10741 agcggctcgt cttcgccctc ccacagcacg caggtcgagc ctttcctgcc cgcgtgcgcc
    10801 agctgaatgc cgatctttgc cacgctctcg ctgtgaacga agtccacgat ccgcttccac
    10861 ccggcgacgt gctcggggag gtacatcccg gcacatccgg gtgagatccg ggcctcccgg
    10921 ctcacatcgg tcatctccgt catcaccaat cccgcgccgc cgatcgcacg gctccccagg
    10981 tgaaccaggt gccagtcgtt gggcattccg tcctcggccc gatactggca catggaggag
    11041 accacgatgc ggttctgcag aaccagctcg cgcaatcggt acggcgtaaa caacggcgac
    11101 gggcacggat ccaccggcac ctggcaccta ctctgcttcg cggactgctc ggcaaaccag
    11161 cggtccaccc gcgccatcag cgccgggtcg cgcaacttca aattctcgtg ggtgatgcgg
    11221 aggctgcggg taagcaggct gaaggc
//
LOCUS       DASRJQ010000385         4447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1642424, whole genome shotgun sequence.
ACCESSION   DASRJQ010000385 DASRJQ010000000
VERSION     DASRJQ010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1642424"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..1047)
                     /locus_tag="VFB89_08610"
     CDS             complement(127..1047)
                     /locus_tag="VFB89_08610"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM27406.1"
                     /translation="MIETSELAQLAGFLAASVRVATPLLLAGIGETVTERSGVINLGL
                     EGAMLAGALASAIGASNAGPWAGVVSAMVAGALVAAVFAACVVFAMVNQIVAGAAVTL
                     ACIGLTGTIYRQTYGAGGVGLSLPTLEQVALPGLSRLPVVGPAFAQPTLTYIAVALVP
                     VVWWGLYRTRWGLGLRASGEAGSLARAAGVRVRLLRFWATVFGGALGGLAGATLVLAQ
                     VGTFTEKMTAGRGYIAIAIVVLGRWHPGGVALAALLFGAATALQFLFQSLGLAVPYQF
                     FLMLPYLLTLLALAGVVGTVRAPAALAVEE"
     gene            complement(1044..2096)
                     /locus_tag="VFB89_08615"
     CDS             complement(1044..2096)
                     /locus_tag="VFB89_08615"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM27407.1"
                     /translation="MTRWPLLVGTAALLAGLGVLALGLQLSGYDAALALAALVQGSFG
                     SWYVISSATLVRAVPLIVIGLGIAIAFRANALNIGAEGQFYAGAIAATWVGLHLGGWN
                     RLLAIPTVWLAAGTAGVLWIVLPVWLKRRFGVLEVISTLLLNFVAESLVSWAVQGPLQ
                     ETRRVYPQSDPIAEAARLPLVPGTRLHLGFFLALGLALVVQYIFRRTIWGFDLLATGS
                     GPKAAEVSGGVKVSRVAGFALLWSGLFAGLAGGIEVGGVSYALFQNLSPGYGFSGIAV
                     ALLARLEPVAILLTGVVFGALEAGGAAMQRDAGVPSVAVYVVEGVVILVLLLAEAALR
                     RIRSAVVLPEAEAGPA"
     gene            complement(2093..3595)
                     /locus_tag="VFB89_08620"
     CDS             complement(2093..3595)
                     /locus_tag="VFB89_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZM27408.1"
                     /translation="MGVPPTPYRIVSEPVLELVGITKSYGPVHALRGADFMLRAGEVH
                     ALLGENGAGKSTLMKVAFGLTRPDGGRMLVRGRSVFPRSPRQARELGIGMVHQHFTGI
                     AALTVAENIALSAGWEVRPARLRAQVGRLMERVRLPLDPAARTGDLSAALKQRLEILK
                     ALAGDATVLLLDEPTAVLAPAEAGELLQFIRGLAAAGRSVVLITHRLEEALESADRVT
                     VLRSGRVRLTGAASDQTPASLTAAMLGTALGSATVARRAEIGDRPVRLRCIALDLLRE
                     GGHGLAVRDANLSVAAGEILGIAAVEGNGQRELLRALAGIVRPLRGRVECDRPVSFIP
                     EDRTTEALISELDLTRNLVLGVGRTAPWVHGWRLDWDVARQRTEALIREFGIRAPSAG
                     VTAGALSGGNQQKLVVARALERLPRVIVAENPTRGLDVQASRFMWERFRDAAANDVAL
                     IVYSTDLDEILTWATRVLVVAQGRVVSLPADSSRAQVGAMMLGEVAESVS"
     gene            complement(3562..4329)
                     /locus_tag="VFB89_08625"
     CDS             complement(3562..4329)
                     /locus_tag="VFB89_08625"
                     /inference="COORDINATES: protein motif:HMM:NF013909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HZM27409.1"
                     /translation="MRAVLTEVARFLDTFLRVTDVPDEVNALNGLQVENDGHVERVVA
                     AVDASLETIEAIQPGNPTLLLVHHGLFWDGNIPLTGRRWRRLKALLARDTALYAAHIP
                     LDLHPEVGNNVELARLIGLPVEGWFGAYRGVPIGVWGTAPPALAERSALVAALDAALQ
                     TRAYLIPGGPAQVQKMGIVTGGAGSMIAAARGAGLDTFITGEGAHHTYFDATELGLNV
                     IYAGHYATEQLGVQALARRLGTEFNLPWEFHRHPTGL"
BASE COUNT          893 a         1543 c         1350 g          661 t
ORIGIN      
        1 gcgcgtgctc gcgccgatcg aaacgaccgg gctcaccgtc aaggacgtgc acgctctgcg
       61 agaacgggtt cgcaagatga ttctcgaggc ccgcgatgcc ctcagggccg cgcaagcctg
      121 accatctcat tcctcgacgg ccaatgccgc cggcgctcga accgttccca ccacgccggc
      181 cagcgccagc aacgtgagca gatacggtag catcagaaag aattggtacg gcacggcgag
      241 tcccaacgat tgaaaaagga attgaagcgc ggtcgcggcg ccgaacaaca gcgccgccag
      301 tgctactccc cctggatgcc accggcccag caccacgatc gcaatcgcga tgtagcctcg
      361 tccggccgtc attttttcgg taaaggtccc cacctgggcc aataccagtg tcgctccggc
      421 gagccctccc agtgcgccgc cgaacacagt cgcccagaac cgtaatagtc gaacgcgaac
      481 cccggccgca cgcgccaatg acccggcttc gccgctggca cgaaggccga gtccccaacg
      541 cgtacggtac agtccccacc agaccaccgg taccaaggcg acagcgatgt acgtgagcgt
      601 tggctgcgcg aacgcgggcc caaccaccgg aagtcgcgac aatcccggga gtgcgacctg
      661 ttccagcgtc ggcagcgaca ggccgacccc cccggccccg taggtctgtc ggtagatggt
      721 gccggtgaga ccaatgcagg caagtgtcac cgcggcgccg gcgacgatct ggttgaccat
      781 cgcgaatacc acgcaggcag cgaagacggc agcgaccagg gcgccggcca ccattgccga
      841 taccacaccc gcccacggcc cggcgttgct cgctccgatc gcagacgcca aagcaccggc
      901 cagcattgcg ccctccagtc cgaggttgat gaccccggac cgctcggtca ccgtttcccc
      961 gataccggcg agcaggagcg gagtcgcaac ccggaccgac gccgcaagga atcccgccaa
     1021 ctgggccaat tcacttgttt cgatcacgcc ggacccgcct cggcctccgg cagcaccact
     1081 gccgagcgta ttcttctcaa cgctgcctcg gcgagaagca gcacgagaat gaccacgccc
     1141 tccactacat agaccgccac cgagggaaca ccggcatccc gctgcatggc cgcacccccc
     1201 gcctcgagag cgccgaagac cacaccggtg agtaggattg cgaccggctc gagccgggct
     1261 aacagggcga cggcaattcc gctgaaaccg taccccggcg acaagttctg aaataaggcg
     1321 tacgaaacgc cccccacttc gatgccgccc gccaatccgg caaagaggcc ggaccagagt
     1381 agcgcgaacc ccgctacacg gctcaccttg acgccgccgc ttacttcagc cgccttcggc
     1441 ccggacccgg tcgcgagcag gtcgaacccc cagatggtac ggcggaatat gtattgaacg
     1501 acgagcgcca agccgagtgc cagaaagaat ccgagatgga gtcgagtccc cggcaccaga
     1561 ggtaaccgag cggcctccgc gattggatcg ctttggggat agacgcgtct cgtctcttga
     1621 agtggtccct gcaccgccca gctcaccagc gactcggcaa cgaaattgag caggagcgtg
     1681 ctaatgacct cgaggacacc gaatctccgc ttgagccaga cgggcaatac gatccacagc
     1741 acgcccgccg tgcccgcggc gagccagacc gtcggtatcg cgagcagccg attccagccc
     1801 cccaggtgca gcccgaccca tgtagcggca atagcaccag catagaactg cccctccgcg
     1861 ccgatgttga gagcattggc gcggaacgcg attgcgatgc ccagaccgat cacgatcagg
     1921 gggactgccc ggaccagggt cgcagacgag atgacgtacc aagagccgaa cgacccttgc
     1981 acaagcgcag caagggcgag cgcggcgtcg taccccgaca gctgaagacc gagcgctaag
     2041 acacccaggc ctgcgaggag cgccgctgtc ccgaccagca gcggccatcg cgtcatgaaa
     2101 cactctcggc gacctcaccc agcatcatcg ccccgacttg agcacgggag ctgtcggctg
     2161 ggagggacac gactcgacct tgtgcgacca ccagaacgcg ggtcgcccat gtgaggatct
     2221 cgtccaaatc cgtggagtag acgatcagcg cgacgtcgtt ggctgccgca tcgcgaaatc
     2281 gctcccacat gaaacggctc gcctgcacgt ccagtccccg cgttgggttc tctgcgacga
     2341 ttacacgggg taaccgctcc aaggcccgcg ccacaacgag cttttgttga tttcctccac
     2401 tcaacgctcc cgcagtcaca cccgcactcg gcgcacggat accgaactca cggatcaacg
     2461 cttcggttcg ttgtcgagct acgtcccaat cgaggcgcca gccgtgaacc cacggcgcag
     2521 tccgacccac cccgagcacg agattgcggg tgagatccaa ctcggagatc aaagcctcgg
     2581 tggtccgatc ttccgggatg aatgaaaccg gccggtcgca ctccaccctt cctctgagcg
     2641 ggcgcacaat cccggcaagc gcccggagca gttcgcgttg accgttgcct tcgacggcag
     2701 cgatgccgag aatctcaccc gcggcgaccg aaaggttggc gtcgcgcact gcaagcccgt
     2761 ggccgccttc tcgcagcagg tccagcgcaa tgcaacgcaa ccgaaccggt cgatcgccga
     2821 tctcggctcg gcgcgccacg gtggctgatc cgagcgccgt tcccagcatt gccgcggtga
     2881 gggaggcagg agtctgatcg ctggcagctc cggtaagcct cacgcgaccg gaacggagca
     2941 cggtcacccg gtcagccgac tccagcgcct cctccagccg atgggtgatc agcacgactg
     3001 accgtccggc ggctgccaga ccccttatga actggagaag ctcgccggcc tccgccggcg
     3061 ccaggactgc cgtgggttcg tcgagcagga gaaccgtcgc atcccccgcg agcgctttca
     3121 gaatctcgag gcgctgcttg agtgcagcgg ataagtcccc ggttcgcgca gcggggtcga
     3181 ggggaaggcg cacccgctcc atgagccgcc ctacctgagc gcggaggcgc gccggccgca
     3241 cttcccaacc agccgatagt gcgatgttct cggcaaccgt gagcgctgcg atgccggtga
     3301 aatgctgatg caccattcca atgccgagct cgcgggcctg ccgcggggag cgggggaata
     3361 ccgagcggcc gcgcaccaac atccgaccgc catccggccg cgtgaggccg aaagcgacct
     3421 tcatgagtgt tgacttgcct gctccgtttt cgcccagcag agcgtggacc tcacccgcgc
     3481 gcaacatgaa atcagccccg cgcagcgcgt gcacggggcc gtaggacttc gtgatcccaa
     3541 ctaactcgag gacgggttcg ctcacaatcc ggtagggtgt cggtggaact cccatggcag
     3601 attgaattcg gtccccagac gtcgggcgag cgcctggaca cccaattgct ccgtggcgta
     3661 atgacccgcg taaatcacgt tcaagcccaa ctcggttgca tcgaagtatg tgtgatgggc
     3721 tccctcgccg gtaatgaagg tatcgagccc ggcgcctcgc gcggcagcga tcatggaacc
     3781 cgccccgccc gtcacgatgc ccattttttg aacctgagca ggtccgcccg ggatcaaata
     3841 cgccctcgtt tggagcgccg cgtccaacgc tgcgaccaag gcgctgcgct cggcgagcgc
     3901 cggcggagcc gtcccccaga ccccgatcgg cacaccgcga tatgccccga accagccttc
     3961 gaccggaaga ccaatgaggc gcgcgagctc gacgttattg ccgacctcgg gatggaggtc
     4021 gagcgggata tgagcggcat agagggccgt atcccgggcg agcagcgctt tgagtcgacg
     4081 ccaacgccgg ccggtgagcg ggatgttccc gtcccaaaag agtccgtggt ggaccaggag
     4141 cagggtgggg ttgcccggtt gtatagcctc gatggtttcc aggctggcgt cgaccgcggc
     4201 aactactcgt tcgacgtgtc catcattctc gacctgaagt ccattcaggg catttacttc
     4261 gtcggggacg tcggtaaccc ggagaaaggt gtccaagaac cgggctacct cggtaagaac
     4321 ggccctcatg gaccggggcg gtctccggca cgggagctgt cccatgccga ggcgggttgc
     4381 agatgaaggg taccagccgc aatcgagtcc gctgccgcct tgacccgagc cttgagtgcc
     4441 aacggga
//
LOCUS       DASRJQ010000386         4291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_333233, whole genome shotgun sequence.
ACCESSION   DASRJQ010000386 DASRJQ010000000
VERSION     DASRJQ010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333233"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="VFB89_08630"
     CDS             complement(<1..1081)
                     /locus_tag="VFB89_08630"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HZM27410.1"
                     /translation="MDLHFLDADPTAEEREAVDALLGPPSSGWDGGERDEERDAFASS
                     GGHAARARRHLLLPALQAVQSRIGWISEGAFNYICQRLSVPPADAYGVVTFYALLSTV
                     PRPRRVLHVCDDIGCQCKGADKLCAELERTLGPAQQHGPNGHQVEIGDNDAIWLRSPC
                     LGLCDQAPAALLTVAGQEPVERLFGNVDAAKAKQVLAGDLTPSKAPHPKLPQAGDSSL
                     RLLRRVNVLDPTSLDEYRAHGGYEALRIAIEWGPEGVIREVTDAKLLGRGGAAFPTGR
                     KWDAVAHQPVRPHYLICNADESEPGTFKDRVIMEGDPFSVIESMTIAAFATGCEVGFI
                     YVRGEYPLAAERLQGAIDAARARGLL"
     gene            complement(1081..3021)
                     /gene="fdhF"
                     /locus_tag="VFB89_08635"
     CDS             complement(1081..3021)
                     /gene="fdhF"
                     /locus_tag="VFB89_08635"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(2773..2775),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HZM27411.1"
                     /translation="MAMNYTRLTEPLVREGGELRPATWEEALDRAAEGFRRAIAQGGG
                     DATATFSCSKASNEVNYLAQKLVRVGFGSHNVDSCNRTUHAPSVVGLATVFGAGGGTS
                     SYREIEETDVIFLWGANARENHPIFFHRVLKGIHNGARLYVMDPRRTASAQWADHWLG
                     LNVGSDIALSNAMAREIIHSGLANEAFIQRATTGFDTYRASVESYTLEYAERETGVPA
                     EVIREAAHAYARADRAEIAWTLGITEHHNAVDNVLSLINLALLTGHVGTYGSGVNPLR
                     GQNNVQGGGDMGAIPNRLVGFQDWTTEPEVRAKFEKAWGVKLEPKYGRNLTQMFDGMD
                     HGEVRAAYAIGENPAQAEADQHRAVRLLKGLDHLVVQDIFLTKTAELAHVVLPASSSW
                     CEAEGTVTNSERRVQRLRKALEPPGNARDDTWIIAEIAKRFGHDWGHPTAEEIWNEVR
                     SLAPIFAGMSYARLEKEGGLHWPCYDENHPGELFLHSRLWKEPVEGRPAPFSVVEHDP
                     PVERPDAEYPFVLTTGRRLDSYNTGVQTGGYTSPLRRGESLDISPEDAERLGLMNGDP
                     VRVSSRRGSVVAPARIDRSLRAGLVFMTFHFQDEVNVNLLTIDETDPKSGTAEFKACA
                     VRVEPVRSAEPLSAHAALRQGA"
     gene            complement(3167..4069)
                     /locus_tag="VFB89_08640"
     CDS             complement(3167..4069)
                     /locus_tag="VFB89_08640"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZM27412.1"
                     /translation="MLLRQLRYLAALAEEQHFGRAAESCNVTQPTLSSGIKQLEEELG
                     LLIVRRGQRFQGFTPEGERVLGWARRILSDCASLEQDATGLREGLIGRLRLGAIPTTL
                     PIVSALTSPFAREHPQVIITVLSLTSVDIQAGLDDFSLDAGLTYLESEPLVGVRAFPL
                     YHERYFLFTAVDGPYRSRRTVSWKEAARTPLCLLTPDMQNRRILDGHFREVGVTIRPQ
                     IETNSVITLCSHLRSGQWSSVLPQTFLTLFGEPSGSRAIRLVDPEVSHSVGLVVPDRE
                     PLTPSARELVELVGRPEVLAGFGG"
     gene            complement(4078..>4291)
                     /locus_tag="VFB89_08645"
     CDS             complement(4078..>4291)
                     /locus_tag="VFB89_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27413.1"
                     /translation="PDVLAAVGDFVPALAPWRSRLGDRLITAADAPAIGPLVAARLRG
                     DEVIVLKASRGVALERILPSLLERAH"
BASE COUNT          754 a         1434 c         1345 g          758 t
ORIGIN      
        1 cgagcaggcc gcgagctcgc gccgcatcga tcgcgccctg cagccgctcg gccgccaatg
       61 gatactctcc ccgcacgtag atgaatccga cttcgcagcc ggtcgcgaat gccgcaatcg
      121 tcatggattc gatcacgctg aacggatcac cctccatgat gacccggtcc ttgaacgtgc
      181 ccggctccga ttcgtcggcg ttacagatga ggtagtgagg ccgcaccggc tgatgggcca
      241 ccgcgtccca tttcctcccg gtaggaaacg ccgcaccacc acgcccgagc aatttggcgt
      301 cggtcacttc ccgaattacg ccctcgggtc cccactcgat cgcgatgcgg agcgcttcgt
      361 acccgccgtg agcccgatac tcatcgaggc tggtcgggtc gagcacgttc actcgccgga
      421 gcaaccggag cgaggaatcg ccggcctgcg ggagcttggg gtggggtgct ttcgaagggg
      481 tgagatcgcc ggcgagaact tgcttggcct tggcggcgtc gacattaccg aacagccgct
      541 ccaccggctc ttgccccgcg accgtcagca gcgcagccgg ggcttggtcg cagagaccga
      601 ggcatggact tctgagccag atcgcgtcgt tgtcaccgat ttcgacctgg tgcccgttcg
      661 gcccatgttg ctgcgccggc ccgagtgtcc gctcgagctc agcgcacagc ttatctgcgc
      721 ctttgcactg gcacccgatg tcatcgcaca cgtgcaggac acgccggggc cgcggcaccg
      781 tcgagagcag ggcgtagaat gtgacgaccc cgtaggcatc cgccggtgga accgagagac
      841 gctggcagat atagttgaac gccccctcac tgatccagcc gatccgggat tgcacggctt
      901 gcagcgccgg cagcagaaga tgccgccggg cccgtgctgc gtgtccaccg ctcgaagcaa
      961 aggcgtccct ctcttcgtcc cgttccccgc catcccatcc ggagcttggt ggaccgagga
     1021 gagcatcgac agcctcacgt tcttcggcag tcgggtcggc gtcgaggaaa tgaaggtcca
     1081 ctaggctcct tgtcgcaaag ccgcgtgagc gctcagcggc tcggccgacc ggactggctc
     1141 gacccgcacc gcgcaagcct tgaactcggc agtccccgat ttcggatcgg tctcgtcgat
     1201 cgtcagaaga ttgacgttca cctcatcctg gaaatggaac gtcatgaaga cgagacctgc
     1261 acgtagcgac cgatcgattc gggccggcgc gaccacgctt ccacggcggg agctgacccg
     1321 taccggatcg ccgttcatga gccctaaccg ctcagcgtct tccggcgaga tgtcgaggct
     1381 ttcccctctt cgaagcggag aggtgtaccc cccggtctga acgccggtgt tatacgaatc
     1441 caggcggcga ccggtcgtga gcacgaacgg gtactccgcg tccggccgtt ccaccggcgg
     1501 atcgtgctcg accaccgaga acggcgccgg ccggccctcg acgggctcct tccacagccg
     1561 gctgtgcagg aacagctcgc ccggatgatt ttcgtcgtag cagggccaat ggagaccgcc
     1621 ctccttttcc agccgcgcgt aactcatgcc cgcaaagatc ggagctaggc tacgcacttc
     1681 gttccagatc tcctcggccg tagggtgacc ccagtcgtgc ccaaaccgct tcgcgatctc
     1741 ggcgatgatc caggtatcgt cgcgcgcgtt gccgggtggc tcgagggcct tacgaagccg
     1801 ctgaacccgt cgctcgctgt tcgtgactgt tccctcggcc tcgcaccagc tggatgaagc
     1861 cgggaggacc acgtgggcca gctcggccgt cttggtgagg aaaatgtcct gcacgacgag
     1921 gtgatcgagg cctttcagca gtcgcactgc ccggtgctgg tcagcctcgg cctgggctgg
     1981 attctccccg atcgcgtacg ctgcacggac ttccccatgg tccatgccgt cgaacatctg
     2041 ggtcaggttg cggccgtact tgggctcgag cttcaccccc cacgccttct cgaacttggc
     2101 gcgcacctca ggctctgtgg tccagtcctg gaaccccacc agccgattgg ggattgcgcc
     2161 catgtcgccg ccaccctgca cattgttctg gccgcggagc ggattgaccc cggagccata
     2221 cgtgccgaca tgccccgtga gcaacgcgag gttgataagg ctcaggacat tgtcgaccgc
     2281 attgtgatgc tctgtgatac cgagcgtcca ggctatttcc gcacgatcgg cgcgggcata
     2341 cgcgtgcgcc gcttcccgaa tgacctccgc cggaacgccg gtttcccgct cggcgtactc
     2401 cagggtgtac gactcaaccg atgcccgata ggtgtcgaac ccggtcgtcg cccgttggat
     2461 gaaggcttcg ttggccagcc cgctgtggat gatctctcgc gccattgcat tcgagagtgc
     2521 gatgtcgctc cccacgttga gcccgagcca gtggtcggcc cactgggcag acgccgtgcg
     2581 tcgggggtcc ataacgtaga gcctggcccc gttatggatt cccttgagga cacgatggaa
     2641 gaagatgggg tgattttccc gggcgttcgc gccccagagg aagatcacgt ccgtctcttc
     2701 gatctcccgg taggagctgg ttcccccacc ggcaccaaac accgtcgcca gaccgacgac
     2761 gctaggagcg tgtcaggtcc ggttgcagct gtcgacgttg tggctgccga agcccacccg
     2821 caccagcttt tgcgcgaggt agttgacctc attgctggcc ttcgagcagc tgaaggtggc
     2881 ggtagcatcg ccgccgccct gggcgatcgc gcgccggaac ccttccgcgg cacgatcgag
     2941 cgcctcctcc cacgtggcag ggcgaagctc accgccctcg cgaaccagcg gctcggtgag
     3001 acgggtgtag ttcatcgcca tgaagtcctc ctggaagccc ttggcgggac gtaacaagcg
     3061 gcccgtggct gaattatgga ccggtgtgct cccgccatca aatttgcgac acctattagc
     3121 tgataggcaa accgaatctt gccgcttggg gcgcctgccg gaggactcac ccgccgaaac
     3181 cggccaggac ctcagggcgg ccgaccagtt cgaccagctc cctggccgat ggcgtgagcg
     3241 gctcgcggtc gggcacaacc agtcccacgc tgtgggagac ctccggatcc accagacgga
     3301 tggcgcgaga gccggatggc tccccaaaca gtgtgagaaa agtctggggc aacacactgg
     3361 accattgccc cgaccgcagg tgggagcaaa gcgtaatgac agaattggtc tcgatctgcg
     3421 ggcgaatcgt cacaccgact tcccggaaat gcccgtcgag aattcgccgg ttctgcatgt
     3481 ccggcgtcag gaggcacagc ggggtccttg cggcctcctt ccacgaaacc gtccggcggc
     3541 tccggtaggg cccatccact gcggtaaaga ggaagtagcg ctcatggtag agaggaaagg
     3601 cccggacgcc caccagtggc tcgctctcca gataggtaag gccggcatcg agactgaagt
     3661 cgtcgagccc cgcctggatg tctacggagg taagtgacag cacggtgatg atgacctgcg
     3721 gatgctcgcg cgcaaagggc gaggtcagcg cagatacaat aggaagagtg gttgggatcg
     3781 cacccagccg cagccggccg atcaagccct ctcgtagccc ggtggcgtcc tgctccaggc
     3841 tggcgcaatc cgacagaatc cggcgggccc agccgagtac ccgctctccc tccggcgtga
     3901 agccctgaaa ccgctggccc cgccggacga tcagcaggcc cagctcttcc tctagctgct
     3961 tgattccgct ggacagcgtg ggctgggtca cgttgcagga ctccgccgcc cgaccgaaat
     4021 gttgctcctc ggccagcgcc gccagatacc gtagctgtct cagcagcacg ggtgtgccta
     4081 gtgagcccgt tcgagcaggc tgggaagaat tcgctcgagc gcgacgcctc gagatgcctt
     4141 aaggacgatc acttcgtcgc ctcgcagcct tgcggccaca aggggaccga tggcgggcgc
     4201 gtcggctgcc gtaattagcc gatcgcccag gcgcgatcgc cagggcgcga gtgccggcac
     4261 gaaatcgccc acggccgcga gcacatcggg c
//
LOCUS       DASRJQ010000387         9611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1469358, whole genome shotgun sequence.
ACCESSION   DASRJQ010000387 DASRJQ010000000
VERSION     DASRJQ010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 9611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9611
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1469358"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1503)
                     /locus_tag="VFB89_08650"
     CDS             complement(139..1503)
                     /locus_tag="VFB89_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZM27414.1"
                     /translation="MSDRVLIVDDEAGIRQALKQVLDYEGMEVRVASSGGEGITVYQD
                     YRPHVVFLDVKMAGLDGLETLTRLRDIDPTATVVMISGHGTIATAVEATQRGAFDFLE
                     KPLDADRLLVTVRNALRHARLVGENERLREDQASRYAMVGDSAALKQVHELVARVGPT
                     GARVLITGENGTGKELVARALHEASPRREQAFIEVNCAAIPSELIESELFGHMKGSFT
                     GAFADRAGKFEQADGGTLFLDEVGDMSLAAQAKLLRVLQEGVVTRIGGAKAVQVDVRV
                     LAATNKDLGLEIDEQRFREDLLYRLNVVEINVPPLRERLEDVPALVHHFTLQLAQTAG
                     VPAKQFADEAIARLQARAWPGNIRELRNAVERALILGSGRIVTAADIGRLFPGAEGAV
                     APPEASRAQTFESFKQEAEKSFLQAKLREHDWNVSETARSLKMPRSNLYKKIERYGLS
                     RESP"
     gene            complement(1500..2132)
                     /locus_tag="VFB89_08655"
     CDS             complement(1500..2132)
                     /locus_tag="VFB89_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27415.1"
                     /translation="MGCLLAPFRALGCLIVVAAVVLAWLYRDRVLSAAMHLAGHRVEA
                     PAAAGRPGTRALENAKARTDSVRRGSVDSVVLSAAETASLIGQGLDPSVRRQLDSLEV
                     RLLDGQIEVGARVATANLPRRALGPLAMALQAHERVRATGPVTVVGSGRGEWRIEQLN
                     LAGVPLPVDAIRPLLEKTFGQQTLPIVIPPGVTDIRIRPTGTVLFGARNP"
     gene            complement(2132..2686)
                     /gene="thpR"
                     /locus_tag="VFB89_08660"
     CDS             complement(2132..2686)
                     /gene="thpR"
                     /locus_tag="VFB89_08660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HZM27416.1"
                     /translation="MRLFAALPILDPGRAAIISLLDELRRSDWPVRWVRAEGIHLTLK
                     FYGEVMPERLDVIEEAVRFAAAGARPIPLKLAHLGAFPTSARPRVLWIGLEAPATLEL
                     LQDRLERGGEEIGFPPEGAPYRPHVTLGRVREGHRLPPGAIDRYELRNEGPSFLAEEL
                     VLYESVLTSDGPRYQARLILPFEA"
     gene            complement(2686..3495)
                     /gene="larE"
                     /locus_tag="VFB89_08665"
     CDS             complement(2686..3495)
                     /gene="larE"
                     /locus_tag="VFB89_08665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HZM27417.1"
                     /translation="MPTLPALEEHLAGLGRVVLGYSGGVDSTLLAVAARRALGPERML
                     AVIGRSASYPAAQRDAAVALANQFDVPLLELETRELNDPQYVANTPERCYFCKTELWN
                     RLGEIANARGFDVVIDGTNADDLGDHRPGLRAALEHAVHSPLAELGWRKSDVRAAARE
                     LGLPVWDAPASPCLSSRIRYGLAVTPARLRQVEAAEAHLRTLGIDGDLRVRHLGAAAS
                     IEVRAEHHARLRDSWDALEAFFVSLGFNTVELDPRGYRRGSLLAIAGESIR"
     gene            3568..4002
                     /gene="arfB"
                     /locus_tag="VFB89_08670"
     CDS             3568..4002
                     /gene="arfB"
                     /locus_tag="VFB89_08670"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HZM27418.1"
                     /translation="MPPEPFLEVSPTLRIPLAELVYRASRSSGPGGQHVNTSSTRIEV
                     VWDIGASSAVTEDQRARLLVRLASRLDSTGRIRLTSGETRSQLRNRETVTKRLRDLVA
                     RALVAPKRRKPTRVPAAAKAARLEAKRRRSDLKRTRRRPDDD"
     gene            4064..6016
                     /locus_tag="VFB89_08675"
     CDS             4064..6016
                     /locus_tag="VFB89_08675"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HZM27419.1"
                     /translation="MPASVAGQATASRSAAEWPAYGLDPEGSRFSPLTDITPANVGSL
                     RIVWRYSTGEASGPEPHRPTSLEATPIVVDGTLFFSTPLGKVIALDPETGSERWVFDA
                     KVGREHTFGDWTSRGVSTWLDANAKPQDSCRRRIIVATVDARLIALDAATGSLCRQFG
                     ANGTVDLRRGLRNAPFEMEEYEETSPPAVVSNVIVVGSGVADNNRTEAASGEVRGFDA
                     RSGRLLWTWDPVPQSSRDPAYPTWQGPEAHRTGAANAWSVMAADPARGLVVIPTGSGS
                     PDYFGGKRLGQNRYANSVVALRASTGTLIWSFQTVHHDLWDYDNASPPALVTITKDST
                     STPAVLQATKSGMLYVLNRETGEPLWPVEERPVPASNVLGEQAWPTQPFSTVEPLSPH
                     RIPAAWGASDGDRTACQTRLGRLRNEGVFTPPSLGGTLVVPSNIGGAHWGGLVFDPSR
                     QLAIVPVNRLATEVRLIPREQLKSVRDSETGSRVEAEYTDMHGTPYVMRREIVRSPSG
                     LPCTPPPFGELVAVSLATGRAVWRVPNGTFPKLSDAASAPPASWGSPGLGGPIATASG
                     LVFIASTPDRFLRAYETATGRELWKGTLPAGGKATPMTYRARPGGRQFVVIAAGGSGG
                     LWGKGDELIAFALPQGVTETTRLRIP"
     gene            6086..7243
                     /locus_tag="VFB89_08680"
     CDS             6086..7243
                     /locus_tag="VFB89_08680"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HZM27420.1"
                     /translation="MPRFTLLSLLAFLPATLLAQGRAEKAAFITRLGSDTLSVEQFSR
                     TSSRLESDVAARVPRARRLHFVVALNPDHTVSRLQVTVQPAVPAPDAPAFTGDMVFGK
                     DTVTMTIKQTDSTITLHVPAGAGAMPLTSTSYALYEQMFLRFQKAGKDSLPFLIVPFG
                     GNQATETSIVRRGADGADYDFFGLPMHAKLDKQGRLLGLDGRESTSKVLVERVKSADV
                     EKAYKDFAARDASQGAMGQLSARDTVRATVGNAHLTIDYGRPHKRGREIFGTVVPWGQ
                     VWRTGANAATGFTTDADLVVGSQTIPKGSYTLWSLPAQNGSKLIINSQTGQWGTDYHA
                     DKDFARLDMKTEALAQPVEVFTIAIDPQGDRAGVLKLQWDRTQLSLPFTVQ"
     gene            7338..8036
                     /gene="lepB"
                     /locus_tag="VFB89_08685"
     CDS             7338..8036
                     /gene="lepB"
                     /locus_tag="VFB89_08685"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZM27421.1"
                     /translation="MKLLRSVWDWSKPVAQALVIWFLVTTFLVEPFRVWPTGSMKNTL
                     LVNDFLFVNKFLYGAELPLASARLPAVREPAHGDIVVVRSVENPSLRLVKRVIGLPGD
                     TVAMQAGVLVRNGLRVSEPYATEPDLSRGADLETQRKMRSWQLRYYVGADAMHYAPDL
                     HDWGPLVVPRDSLFLLGDNRDSSYDGRYYGFVPRANLLGHPFVVYFSYDNDSWHSLPF
                     VTAVRWHRLFTQPR"
     gene            complement(8020..>9611)
                     /locus_tag="VFB89_08690"
     CDS             complement(8020..>9611)
                     /locus_tag="VFB89_08690"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZM27422.1"
                     /translation="ARSVGDVVSGPTRRLVLIALDAAEPRLIEQWMGDGSLPNLRVLR
                     GRGAYGRLESSADWLAGSPWPTFYTGTLPSDHGFYHYLRWQADRMQAVRPSPDHLPLH
                     PFWRQLSGLGPRVIAIDPPMTYAAEPFNGVEISGWASHDGLVPPCVYPTDVMRWVEQE
                     VGGSPRTDEEHVALPIQRAFGIRDEQIRAVHMVSKLAVRLMEKERWDLLLAAFSATHR
                     CGHQLWDHTGLAEQPDSADQSAFGDALREVYVACDAALGSIVSAAGDSQIIAFSLHGM
                     GVETTRIDILPEMLRRIITGESSPLPPRKNLLRRVRDWIPNTWRHAVKQRLPVVIQDR
                     LTAFWRLGGANWPRTRAFCQVADLQGYIRINLKGREAAGVVAPGAEFDELCSSITDGL
                     LTFRDADTGEPVVREVVRSDQLFPPGSRRDELPDLLVGWAPSPVAGQRAIVSPQFGQI
                     DLPTPGRNPTGRSGNHRPHGFFIGVGEGFEAGANIEDGHILDLAPTVYAMLNLPCPPT
                     MRGRDLRSLSPRGRPMVSEAE"
BASE COUNT         1699 a         3197 c         3008 g         1707 t
ORIGIN      
        1 tcgcgaccgc cggttgcgga gcgggcaggg ccggcgcgac acccggcttg gccaccggaa
       61 cctcggcagg cggctgttgc cgagccatgg cgcggtcgat ggcggagagc tccttgtccc
      121 agtcccgcgg ctgctcgttc atggcgattc ccggctcagg ccgtagcgct cgatcttctt
      181 atataggttt gatcgcggca tcttgagcga ccgtgcagtc tcggagacgt tccagtcgtg
      241 ctcgcgaagc ttcgcctgca ggaatgactt ctcggcctcc tgcttgaagc tttcgaatgt
      301 ctgagcccga ctcgcctcgg gtggcgccac ggccccttcc gcccccggga acagtcgacc
      361 gatgtcggcc gcggtcacga tccttccgga gccgagaatc agtgcccgtt cgaccgcgtt
      421 gcgaagctcg cgaatgtttc cgggccacgc ccgcgcctgg agtcgcgcga tggcctcgtc
      481 ggcaaactgc ttggcgggaa cgcccgcggt ctgcgccagc tgcagcgtga aatgatgcac
      541 cagcgcgggc acgtcctcca gtcgctcccg taacggaggt acgttgatct ccaccacgtt
      601 cagccggtac agcagatcct cccgaaaccg ctgctcgtcg atctcgagtc ccaggtcctt
      661 gttggtcgcc gccagcactc gaacatccac ctgtaccgcc ttggcgccgc cgatgcgggt
      721 gacgacgcct tcctgcagca cgcgcagcaa cttggcctgg gccgcgagcg acatgtcgcc
      781 cacttcatcg agaaagagcg tgccaccatc ggcctgctcg aacttcccgg cacgatcggc
      841 gaatgccccg gtgaacgaac ccttcatgtg accgaatagc tcgctctcga tcagctcgga
      901 tgggatggcc gcacagttca cttcgatgaa cgcctgttct cgtcgaggcg acgcctcgtg
      961 gagcgctcga gccaccagct ccttgccggt tccgttctcg cccgtgatca ggacacgggc
     1021 gccggtcggc ccgactcgcg ccaccagctc gtggacctgt ttgagcgccg cgctgtcccc
     1081 caccatcgcg tagcgactcg cttggtcttc gcgcagccgt tcgttctcgc cgaccagccg
     1141 agcatgccgc agcgcgttgc gaaccgtcac gagcaggcga tcggcgtcca gcggtttctc
     1201 caggaagtcg aatgcgcccc gctgggtcgc ttctaccgca gtggcgatgg tcccatggcc
     1261 tgagatcatc acgaccgtgg cggtgggatc gatgtcgcgc aggcgagtca gggtctcgag
     1321 gccgtcgaga cccgccatct tcacgtccag aaagaccacg tggggccgat agtcctgata
     1381 gacggtgatg ccttcaccgc cggaagaggc gacccgtact tccatcccct cgtagtcgag
     1441 cacctgcttg agcgcctggc gaataccggc ctcatcgtcg acgatgagca cgcggtcgct
     1501 catgggttgc gcgctccaaa gagaacggtt cctgtaggtc gaatcctgat gtcggtaacc
     1561 cccggtggaa tcacaatcgg cagggtttgc tgcccgaacg tcttctcgag taatggcctg
     1621 attgcgtcga ccggcagcgg aacccccgcc aggttgagct gctcgattcg ccattcgccc
     1681 cgcccactgc cgacaacagt caccggaccg gtcgcgcgga cccgttcatg agcctggagc
     1741 gccatcgcca gcgggccgag cgccctgcgc ggcaggttgg cggtggccac ccgggcgcca
     1801 acctcgatct gtccgtcgag cagccgtact tcgagggagt cgagctggcg acgaaccgaa
     1861 ggatcgagtc cctgaccgat cagggatgcg gtctcggccg cgctcagcac cactgagtcg
     1921 acggagccac ggcgaaccga atcggtccgc gccttggcat tctcgagggc ccgcgttccc
     1981 gggcggcccg ccgctgccgg agcttcaact cgatggccgg ccagatgcat cgctgccgac
     2041 aagacgcggt cacgatacaa ccaggcaagc acgacggctg ccacgacgat gaggcatcct
     2101 aatgcccgaa acggcgcgag caggcagccc atcacgcctc gaatggaagg atcagcctgg
     2161 cctgatagcg aggaccatcc gacgtcaaca cgctttcgta caacaccaac tcctccgcca
     2221 gaaacgaggg tccctcattg cgcagctcgt agcgatcgat tgcaccgggc ggcagccgat
     2281 gaccttcacg gacccggccc agcgtgacgt gcggccggta gggcgcacct tccggcggaa
     2341 agccgatctc ctcgccgcct cgctccaggc ggtcctggag gagctccagc gtcgccggtg
     2401 cttccagacc gatccagagc acccgcggtc gggcggacgt ggggaaggcg cccagatgcg
     2461 cgagctttag gggaatcggc cgagcgccgg ccgccgcgaa acgaactgct tcctcgatga
     2521 cgtcgagcct ttcaggcatc acttcgccgt agaatttcag cgtgagatga atgccctcgg
     2581 cgcgcaccca gcgcaccggc cagtcggacc gccgcagctc atcgagcaga gatatgatgg
     2641 cggcccgccc gggatccaga atcggcagcg cggcaaacaa ccgcatcagc ggatggactc
     2701 gccggcaatg gcgagaagac tgccccggcg ataccctcgc ggatcgagct caacggtgtt
     2761 gaatcccagc gaaacgaaaa acgcctccag ggcatcccag ctgtcgcgca accgcgcatg
     2821 atgctccgct cgtacctcga tcgaagcggc agcgccgagg tgcctcacgc gcaggtcgcc
     2881 gtcgatcccg agcgtccgaa ggtgagcctc ggctgcttcc acctggcgga gacgggccgg
     2941 cgtgacggcg aggccgtatc ggatgcgact ggagaggcac ggcgatgccg gcgcgtccca
     3001 gaccggcaaa cccagttccc gtgccgcggc gcggacgtcg gacttgcgcc agcccagctc
     3061 cgccagtggc gagtggacgg catgctcgag cgcagcgcgc agacccggac gatgatcgcc
     3121 gaggtcgtcg gcattcgtac cgtcgatcac gacgtcgaac ccccgggcgt tcgcgatctc
     3181 gcccagccga ttccatagct cagtcttgca gaagtaacag cgctcgggtg tgttggcgac
     3241 gtactgcggg tcgttcagct cacgcgtctc gagctcgagc agcggcacat cgaactgatt
     3301 tgccagagcg acagccgcat ctcgctgagc cgcgggatac gaagcgcttc ggccgatcac
     3361 ggcgagcatg cgctcggggc ctagtgccct tcgagcggcc actgccagca aggtggaatc
     3421 caccccaccc gagtagccca gcaccacccg gccgagcccg gcgagatgct cctcaagcgc
     3481 tgggagggtc ggcatgggag taacgtaatc cgctcggggc aaggcggaag cgttggtcgc
     3541 cgtgggttga atcgcttacc cttgggcatg ccaccagagc catttctcga ggtcagccca
     3601 acgttgcgca ttcccctcgc cgaactggtg tatcgggcct cccgctcgag cggacccggc
     3661 gggcagcacg tcaacacgtc gtcgaccaga attgaagtcg tgtgggatat cggggcatcc
     3721 agcgccgtga ccgaagacca acgcgcccgt ttactggtga ggctcgcctc tcggctcgac
     3781 tcaaccggcc gcattcgttt aacgtcgggt gagacccgaa gccagctgcg aaatcgagag
     3841 accgtgacca aacgccttcg cgacctggtg gctcgggcac tcgtggcccc caagcgccgt
     3901 aagccaaccc gagttccggc ggcggcaaag gcagcgcggc tcgaagccaa gcgcagacga
     3961 tccgacctca agcggactcg acgtcggccg gacgacgact agggacgagg taatgctcac
     4021 gcgctccaga ctcctgccaa ttcctttcct tctctctgcg cttttgcccg cgagcgttgc
     4081 cggtcaggct acggcgtccc gatccgccgc agaatggccc gcctatggcc tcgacccgga
     4141 agggtctcgt ttcagtccgc tcacggatat cacgccggcc aacgtcggca gtctgagaat
     4201 cgtatggcgc tatagcaccg gcgaagcctc gggacccgaa ccccatcgcc cgacgtcgct
     4261 cgaagcaact ccgatcgtag ttgatggcac actcttcttc agcactccgc tgggtaaggt
     4321 gatcgcactg gaccctgaga ctggttcgga gcgctgggtt ttcgacgcga aggtcggacg
     4381 ggagcacacg ttcggcgact ggaccagccg gggcgtatcc acttggctgg atgccaatgc
     4441 aaagccgcag gattcttgcc gccgacggat catcgttgca accgtcgacg ctcggcttat
     4501 cgcgctcgac gcggccaccg gctcgctatg tcgccagttc ggcgccaatg ggactgtgga
     4561 tctgcggcgt gggctacgta atgcgccgtt cgagatggag gaatacgaag agacctcacc
     4621 acccgcggtg gtcagcaatg tcatcgtggt cggctccggc gtcgccgaca acaaccgcac
     4681 cgaagcggcg agcggagaag tgcgcggctt cgacgctcgc agcggacgct tgctctggac
     4741 ctgggacccg gtgccacaga gctccaggga tccagcgtat cccacctggc aaggtcccga
     4801 ggcacatcgc accggggctg ccaacgcctg gtccgtgatg gcagccgacc ctgcgcgtgg
     4861 tctcgtagtg atccccaccg gaagtgggag ccctgactat ttcggcggga agcgacttgg
     4921 gcagaatcgc tacgccaact cggtcgtagc gcttcgcgca tccaccggca cgctgatctg
     4981 gagtttccag acagtccatc atgatctctg ggactacgac aacgcctcac cgccggcgct
     5041 ggttacgatc acgaaggact cgacctcaac tcccgccgtg ctgcaagcca ctaagagcgg
     5101 catgctgtac gtcttgaacc gtgaaaccgg ggagcctctc tggccagtgg aggaacgacc
     5161 ggttccagcc agcaatgtcc tgggagaaca ggcctggccc actcagccgt tctccaccgt
     5221 ggagccgctg agcccgcacc ggatccctgc cgcctggggc gcgagcgacg gcgaccgcac
     5281 cgcctgtcag acccggctcg gccggcttcg gaatgaagga gtctttacgc caccgagtct
     5341 cggcggcacg ctcgttgtgc cctccaacat cggtggcgcg cattggggcg gcctggtctt
     5401 cgatccttcg cgccagctgg caatcgtgcc ggtgaaccgg ctggcgacgg aagttcgact
     5461 cattccccgc gagcagctga aatccgtcag agattcggag accggctccc gagtcgaagc
     5521 cgagtatacc gatatgcacg ggacgccgta cgtgatgcgg cgggagatcg ttcgatcacc
     5581 gtcgggtctt ccgtgcactc cgccgccgtt cggcgagctg gtggccgtga gcctggcgac
     5641 cggtcgcgcg gtctggcgag tccccaacgg aacattccca aaactttccg acgctgcctc
     5701 ggcgccgccc gcgagctggg gatcgccggg gctcggcgga cccatcgcaa ctgcgagcgg
     5761 actcgtgttc attgcctcca cgccggaccg ctttcttcgc gcgtatgaga cggccacggg
     5821 gcgtgagcta tggaaaggca cgctgccagc cgggggcaag gccactccga tgacctaccg
     5881 cgcccgaccc gggggacgtc agttcgttgt gattgccgcg ggaggctcgg gcgggttgtg
     5941 gggcaaaggg gatgagctga tcgcttttgc tctcccccag ggagtgaccg aaaccacccg
     6001 ccttcgcatc ccgtaagaga ccttgcctcg taattggcag cggtggtcga tgaggctttc
     6061 ctatctccta atctcggatg cgctcatgcc acgcttcact ctcctgtcgc ttctcgcgtt
     6121 tcttcccgcg acgttgctgg cccagggccg cgccgagaaa gcggccttca ttacccgtct
     6181 cggcagcgac actctttccg tcgaacagtt ctcccgcacc agtagccgcc tcgagtcgga
     6241 tgtcgccgca cgggtaccgc gggcccgtcg cctgcatttc gtcgtggcgc tcaatccgga
     6301 ccataccgtc tcccgactgc aggtgacggt gcagccggcg gtccccgctc cggacgctcc
     6361 tgcgttcact ggtgacatgg tcttcggcaa ggacaccgtg accatgacca tcaagcaaac
     6421 cgacagcacg attacgctac acgttccggc tggagccggt gctatgccgt tgaccagcac
     6481 atcatacgcg ctgtatgagc agatgttcct tcgcttccag aaagccggca aggacagcct
     6541 tcccttcttg atcgttccgt tcggcggcaa ccaggcaacc gagacgtcga tcgtgcggcg
     6601 aggagccgat ggcgccgact acgacttctt cgggctgccg atgcatgcca agctcgacaa
     6661 gcagggccgg ttgctcgggc tcgatggccg ggagagcacc agcaaggtgc tagtcgaacg
     6721 ggtcaagtct gccgatgtgg agaaggcgta caaggatttc gccgcccggg acgcatctca
     6781 gggcgccatg gggcagcttt ccgcccgcga cacggttcgt gccaccgtcg gcaatgctca
     6841 tctgacgatc gactacggcc ggcctcacaa gcgggggcgt gagattttcg ggaccgtcgt
     6901 tccgtggggt caggtctggc gcaccggtgc gaacgccgct accggattca ctaccgatgc
     6961 cgatctggtc gtaggatcgc agactatccc gaaaggcagc tatacgttgt ggagtttgcc
     7021 agctcagaac ggaagcaagc tgatcatcaa ctcgcagacc gggcagtggg gaactgatta
     7081 tcatgccgac aaggactttg cccggctcga tatgaagaca gaggcgctgg ctcagccggt
     7141 cgaggtgttc accattgcaa tcgatccgca gggagatcga gctggcgtcc tcaagttgca
     7201 gtgggaccgc acccagcttt cccttccctt caccgtgcag tagcgcgcag ccactcgcca
     7261 tttgcccggt gtcgaatcag gcaccgggct gatggccccg cgatccagct ctttacttcg
     7321 aggacacgcc gttgtttgtg aagttgctcc gctcagtttg ggattggtcc aagcctgtcg
     7381 cccaggctct ggtcatctgg tttctggtta caacctttct ggtcgagccg tttcgagtct
     7441 ggccaaccgg tagcatgaag aacacgctcc tggtgaacga ctttctcttc gtcaacaagt
     7501 tcctctacgg ggccgagctg ccgctcgcgt cggcccggct gcctgcggtg cgcgaacccg
     7561 cccacggcga catcgtggtc gtacggtcgg tcgagaatcc gtcacttcgc ctggtgaagc
     7621 gcgtgatcgg acttcccggc gacaccgtag cgatgcaggc cggcgtgctg gtccgcaacg
     7681 gactccgggt gtcggagcca tacgccacag agccggacct gagtaggggc gcggatctcg
     7741 agactcagag aaagatgcgc agctggcagc tccgctacta cgtcggtgcg gacgcgatgc
     7801 attacgcgcc cgatctccac gattggggcc cgttggttgt tccccgcgac tctctttttc
     7861 tattgggcga taatcgggac tcttcctacg acgggcggta ctacgggttc gtgccgaggg
     7921 caaacctgct gggccatccg ttcgtggtct acttcagtta cgacaacgat tcttggcact
     7981 cgcttccgtt cgtgaccgcg gtccgctggc accggctctt tactcagcct cgctgaccat
     8041 gggcctgccc ctggggctga gcgaccgcag gtcgcggccg cgcattgtgg gtggacacgg
     8101 caggttgagc atcgcgtaga cggtcggggc aaggtcgaga atgtggccgt cctcgatgtt
     8161 ggcccctgcc tcgaatcctt cacccacgcc gatgaagaag ccgtgtggtc ggtgattgcc
     8221 gcttcgcccg gttgggtttc tgcccggcgt gggcaggtcg atctgtccga actgcggcga
     8281 cacaatggcg cgctgccccg cgactgggct cggcgcccat ccaacgagga gatccggcaa
     8341 ctcatcccgt cgcgaacccg gcggaaagag ctgatcgctc cggacaacct cacgcaccac
     8401 cggctccccc gtgtcggcgt cgcgaaacgt gagcagtccg tctgtgatgc tggagcacag
     8461 ctcgtcgaac tcggcgcccg gcgcgaccac accggcggcc tctctccctt tcaagttgat
     8521 gcgtatgtac ccctggagat ccgctacctg gcagaaggcc cgtgtgcgtg gccagttggc
     8581 tccgcccagg cgccaaaagg cggtcaggcg atcctggatg acgaccggga gtcgctgttt
     8641 tactgcatga cgccaagtgt tcgggatcca gtctcgcacg cgacgaagaa ggtttttgcg
     8701 gggcggcagc ggcgaggatt cgccggtgat gattcggcgc aacatctccg gaagaatatc
     8761 aatccgggta gtctccacac ccatgccgtg gagagagaac gcgatgatct ggctgtctcc
     8821 ggcggcagaa acgatgcttc ccagtgccgc gtcacacgcg acgtacacct cgcgcaacgc
     8881 atccccaaag gccgattgat ctgccgaatc cggctgttcc gccagcccgg tatggtccca
     8941 cagctggtgc ccgcagcgat gtgtcgcgct gaacgccgcc aggagcaggt cccagcgttc
     9001 cttttccatc agtctcacgg cgagcttgct caccatgtgc accgcgcgaa tctgctcgtc
     9061 ccgaattcca aatgcacgtt gaatcggcag agccacatgc tcctcgtcgg tcctgggaga
     9121 gccacccacc tcttgttcca cccatcgcat cacgtccgtg gggtaaacgc acgggggaac
     9181 caggccatcg tgagaggccc atcccgagat ctcgacgcca ttgaatggct cggcggcgta
     9241 cgtcatcggt ggatcgatgg cgatgacgcg gggaccgagt ccgctcagtt gccgccagaa
     9301 tggatggaga gggaggtgat caggcgacgg gcgtacggcc tgcatccgat ccgcctgcca
     9361 ccgcaggtaa tggtagaagc cgtgatcgct gggaagcgtg ccggtgtaga aggtgggcca
     9421 gggagagccc gcgagccaat cggctgacga ctcaagcctg ccgtaggctc ctcggccacg
     9481 gagcactctc agattaggca acgatccgtc gcccatccat tgctcgatca gacgaggctc
     9541 cgccgcatcg agcgcgatga gaaccagccg ccgcgtcggt ccactcacaa catcaccgac
     9601 tgaccgcgcc a
//
LOCUS       DASRJQ010000388         2083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2036141, whole genome shotgun sequence.
ACCESSION   DASRJQ010000388 DASRJQ010000000
VERSION     DASRJQ010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2036141"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VFB89_08695"
     CDS             <1..632
                     /locus_tag="VFB89_08695"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HZM27423.1"
                     /translation="PTGRRVAVHLAVGKITATNVNGRIELETSNGPVVVNGSKGEVEV
                     QVGSGDVQLSASEGDLEVTTGSGDVQATKSNGRSISIQTGSGDVVASQLQATEVAVRT
                     GSGGIKASGLKAPQVGLETGSGDVTVELQGTVDRLDVRTGSGNIAITAPANLGAQVDM
                     QTASGDIDSDFPLSVTRSGGDHLRGTVGDGKGRISMETGSGGVRLLKSR"
     gene            complement(629..1045)
                     /locus_tag="VFB89_08700"
     CDS             complement(629..1045)
                     /locus_tag="VFB89_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27424.1"
                     /translation="MPKGTPVERALGSLYMVMNRAVTHHSPKIQDAQTQEEQLALAIT
                     YCFDIQRKLEYACHALEAFAEYGDPELAHQLLKDDEELCNTGISWEQRKHIAHRIMLE
                     VYAAIYEKHESKVKEHAGRATRRAPVPAGRKRIDTR"
     gene            complement(1085..>2083)
                     /locus_tag="VFB89_08705"
     CDS             complement(1085..>2083)
                     /locus_tag="VFB89_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27425.1"
                     /translation="IDAQPAFDRLVALDSTFAPIYDHLADLALDSGQLDRAAQFVRRI
                     PIDDPGRPARLAAIALQRASGPTRAELLDSLTHADRYALSDLVALMVRDSSHVSLADS
                     IAAILGGRDRTPDDRRRAADYRLAALARLGRWPEAVRTWKAAASRGPLDGWMILAGFA
                     GFQADSFTAPMFAWARATTLAGRAPDFSLPPTHDYQQAFQALVYRATLQGDSAEVNRL
                     LARLDSAPPSPDLSDGLRETLTYSLRARLGLLAGDTAQAIAFLQRSVVRIPERMVTFL
                     PFAGMAPQRYLLAELLARRQNTAESRRWLASLTDSWAVPDILYAPAALKLEAELQP"
BASE COUNT          367 a          635 c          726 g          355 t
ORIGIN      
        1 tacccacggg gcggcgggtg gcggtgcacc tggcagttgg aaagatcacc gcaaccaacg
       61 tcaacggacg gatcgagctg gagacctcca atggcccggt tgtcgtaaac ggttcgaaag
      121 gagaggtgga agtgcaggtt ggctcgggag acgtgcagct atccgcgagc gagggcgacc
      181 tcgaagtcac tacgggctcg ggtgacgtgc aagccacgaa atcgaatgga cgctcgatct
      241 ccatccagac gggctccggc gacgtcgtgg cgagccaact ccaggcgacg gaggtcgccg
      301 tgcgcactgg atcaggcggg atcaaggcga gcggcctcaa ggcaccgcaa gtcggcctcg
      361 agaccgggag cggcgacgtg accgtagagc tgcaaggcac agtcgatcgg ctcgatgttc
      421 gcaccggctc gggcaatatc gccattaccg cgccggcaaa cttgggggct caggtcgata
      481 tgcagaccgc gagtggcgac atcgactccg acttccccct gtccgttacc cgcagcggcg
      541 gcgaccacct ccgcgggacc gtgggcgacg gcaaggggag aatctcgatg gaaacagggt
      601 cggggggcgt gcgactactg aagtcacgct agcgagtgtc gatccgcttc cgccccgccg
      661 gaaccggagc gcggcgcgtc gcgcgaccgg cgtgctcctt gaccttggac tcgtgtttct
      721 cgtagatcgc ggcgtacacc tcgagcatga tccggtgagc gatgtgcttg cgctgctccc
      781 agctgatgcc ggtgttgcat agctcttcgt cgtcttttag gagctgatgc gccagttcgg
      841 ggtcgccgta ttcggcgaac gcttccaggg cgtgacaggc atactccagc tttcgctgga
      901 tgtcgaagca gtaggtgatg gccagcgcca gctgctcctc ctgagtctgc gcgtcctgga
      961 tcttgggcga atggtgcgtg accgcccgat tcatgaccat atagagactg ccaagcgctc
     1021 gttccaccgg cgtgcccttg ggcattgagg aatctccttg caggggtgta ccgcgaatgt
     1081 ggggctacgg ttgtaactcg gcttcgagct ttagagcggc tggtgcgtac aagatgtccg
     1141 gcaccgccca gctgtcggtc agggaagcca gccagcgccg agactcggcg gtattctggc
     1201 gacgcgctag gagttcggct agcaggtagc gctggggcgc catgccggcg aagggcaaga
     1261 aggtgaccat gcgttcggga attcggacca ccgaccgttg caggaacgcg attgcctgag
     1321 ccgtgtctcc ggcaagcaac ccgagccgcg ccctgagcga ataggtcagc gtttcgcgca
     1381 gtccgtcgga cagatccggg ctcggtggcg ccgagtcgag tcgcgctaac aaccggttga
     1441 cctcggccga gtcgccctgg agcgtggcac ggtacaccag ggcctggaat gcttgctggt
     1501 aatcgtgcgt gggtggcagc gaaaagtcgg gcgccctccc tgccagcgtc gttgcccggg
     1561 cccaggcaaa catcggagcg gtgaagctgt ccgcctgaaa acccgcaaac cctgccagaa
     1621 tcatccaacc gtccagcgga cctctgctgg ctgccgcctt ccaggtcctg accgcttcag
     1681 gccagcggcc cagccgcgca agagcggcca accggtagtc ggcggcccgc cttcgatcgt
     1741 ccggggttcg gtctcggccg cccagtatcg cggcgatgga atcggccagc gatacgtgac
     1801 tcgagtcgcg aaccatcagc gcgaccaggt cggacagcgc ataccggtcg gcgtgcgtga
     1861 ggctgtccag cagctcggcg cgggttggtc cgctggctcg ttgaagtgcg atcgcggcga
     1921 ggcgggcggg gcgcccgggg tcatcaatcg ggattcgccg tacgaactgg gcggcccgat
     1981 ccagctgtcc gctgtcgagc gcgagatccg cgagatggtc gtagatcgga gcgaaggtgc
     2041 tgtcgagggc gaccagccgg tcgaatgccg gctgggcatc gat
//
LOCUS       DASRJQ010000389         2171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_701558, whole genome shotgun sequence.
ACCESSION   DASRJQ010000389 DASRJQ010000000
VERSION     DASRJQ010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701558"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="VFB89_08710"
     CDS             <1..1080
                     /locus_tag="VFB89_08710"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZM27426.1"
                     /translation="LAGLTPRCSSRPERWCVYGRSARLPATIQTEVSMRRIGLAVALT
                     VSLALAPLVAETQTAGKVHRIGLLATHISQQAGRVEALRAGLRDLGYIEGQNVVIEYK
                     WADGRNDRFPDLAAELVRLNVDVLVTSGTPATQAAMQATTTIPIVMVNTGDPLRTGLV
                     ASLARPGGNVTGLTGLGAEVAGKRLQILKDTVPKVSRVAFLWNRVNASHRPYFNDLQA
                     AARELKLTLQSVEVQELSEFERAFAEMMRERPDALLVTADGMHRLHQAWIVDFAAKRR
                     LPTMYQLKDYVEAGGLMSYGTSITEPWRRAATYVDKILKGAKPADLPVEQPTKFELVI
                     NLKTAKALGLTIPQSVLLQADQVLQ"
     gene            1160..2134
                     /locus_tag="VFB89_08715"
     CDS             1160..2134
                     /locus_tag="VFB89_08715"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZM27427.1"
                     /translation="MRRIGLAVGLAVSLTVAPLAGVAQQRQVPRLGVLGIDSMSPRPV
                     AFLDELRVLGYLEGRTITIEYRWAEGRPDRLPALAAELVALPVDILVTSGGAPVVRAA
                     KQATTSIPIVAMIMNEPVEAGLVSSLARPGGNVTGHAFEASAISTKQLELLSETVPKL
                     SRVAVLWYEGGNQEAGTVRAAQDAARTLRLQLQAHEVRGLADLVGAVAAAKAWGAHAL
                     LQIPSPFFFNHRATLVALLTAHRIPAMCESRPLVVEGCLMTYAPSFEAMARRTAYYVD
                     RILKGAKPADLPVEHPTKFEFVINLKTAKALGLTIPQTLLQRADQVIE"
BASE COUNT          383 a          717 c          705 g          366 t
ORIGIN      
        1 cttgctggcc tcacgccccg gtgctcgtcc aggccggagc gctggtgcgt ctatgggcgt
       61 tcggctagac tcccggcaac catccaaacc gaggtgtcca tgcggcggat cgggctcgcg
      121 gtcgctctca ctgtcagcct cgctctcgcg ccgctcgtcg ccgagacgca gacggcgggg
      181 aaggtccacc ggataggact gttggctacg catatcagtc aacaagcagg tcgggtggaa
      241 gcactgcggg cgggtctgcg cgacctcggc tatatcgagg ggcagaacgt cgtcatcgaa
      301 tacaaatggg ccgatgggag gaatgatcgg ttccccgact tggcggccga actggtgcgt
      361 ctcaacgtgg atgtcctcgt gacaagtggc acgcccgcaa ctcaagccgc catgcaagcg
      421 acgacaacga ttcctatcgt tatggtgaac accggcgatc ctcttcggac cgggctcgtc
      481 gcgagcctcg cgcggccggg cggcaacgtc acgggattga ccggactcgg cgccgaggtc
      541 gccgggaaac ggctgcagat cctcaaggac actgtgccca aggtctctcg cgtggccttt
      601 ctatggaatc gggtgaatgc atcccataga ccctacttca acgaccttca ggccgcggca
      661 cgggagttga agttgacgct gcaatcagtt gaggtgcagg agctctccga gttcgagagg
      721 gcgttcgccg agatgatgcg ggaacggccc gatgcgctcc tcgtgactgc cgacggcatg
      781 caccgactcc accaggcgtg gattgtcgac ttcgccgcga agaggcggct accaacgatg
      841 taccagctga aggactatgt cgaggccggg ggcctcatgt cctacggcac gagcatcacc
      901 gaaccctggc ggcgcgcggc cacctatgtg gacaagatcc tcaagggggc caagcccgcc
      961 gacctgccag tcgagcaacc gaccaaattc gagttggtca tcaacctcaa aactgccaag
     1021 gcgctcggtc tgacgatccc gcaatcagtg ctgctgcaag ccgaccaggt cctccagtag
     1081 ccgtgcgctt caacgcagcc gctgccgcgc tcagagtcgt ctgctagact ccccgccagc
     1141 cttcctccac gaggtgccca tgcggcggat cgggctcgcg gtcggtctcg ctgtcagcct
     1201 cactgtagcg ccactcgccg gcgtggcgca gcagaggcag gtgccacggc tcggggttct
     1261 gggcatcgac tccatgagcc cgcgaccggt tgcctttctc gacgaactgc gcgtcctcgg
     1321 ctacctggag ggtcgaacca tcacgatcga gtaccgctgg gcggaggggc gcccagaccg
     1381 cctgcccgcg ctcgcggccg agctggtcgc gctcccggtg gacatcctgg tcacttccgg
     1441 aggagccccg gtcgtccgcg ccgccaagca ggccacgacc agcattccga tcgtggccat
     1501 gatcatgaac gagccggtgg aggccgggct cgtctccagc ctggctcggc cgggcggcaa
     1561 tgtgacagga catgcgttcg aggcctcggc gatcagcacc aagcagctcg agctgctcag
     1621 cgagacagtc cctaagctct cgcgtgtcgc ggtgctgtgg tacgagggcg ggaaccagga
     1681 ggccggcacg gtgcgagcag ctcaggatgc ggcaaggacc ttgaggcttc agctgcaggc
     1741 ccacgaagtg agggggctgg cggacctcgt tggcgcggtc gccgccgcca aggcgtgggg
     1801 cgcccacgcc ctgctccaga tcccgtcccc gttcttcttc aaccaccgcg cgaccctcgt
     1861 ggcactcctt accgcgcatc ggattcccgc gatgtgcgag tcgcggccgc tagttgtcga
     1921 gggatgcctc atgacttacg cgccaagctt cgaagcgatg gcgcgtcgaa ccgcgtacta
     1981 cgtggaccgc atcctgaaag gtgcgaagcc cgccgaccta cccgtcgagc atcccacgaa
     2041 gtttgagttc gtgatcaacc tcaagacagc gaaggcgctc gggctcacga tcccgcagac
     2101 attgctgcag cgggcggacc aggtgatcga atagcccgcg cttcgtgcgg cagcggtgct
     2161 ggtgcgcgtc c
//
LOCUS       DASRJQ010000390        11620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3899449, whole genome shotgun sequence.
ACCESSION   DASRJQ010000390 DASRJQ010000000
VERSION     DASRJQ010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 11620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11620
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3899449"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /gene="mraW"
                     /locus_tag="VFB89_08720"
     CDS             complement(<1..573)
                     /gene="mraW"
                     /locus_tag="VFB89_08720"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HZM27428.1"
                     /translation="MATGFHNPVLLGPLLDAIGQPSRAVDATLGDGGHAAALREAGAE
                     VLGIDRDPDAIAVARSRLGDSRMTYLEASFGSDAALSAVAAFRPNVVLLDLGVSSRQL
                     DQTERGFSFRPGAELDMRMEGTGHTAADLLNSTEQTTLAEWLSDWADEPRARRLAAEI
                     VRRRNGKPFETSDDLVNAIRAVLGSRAGPSD"
     gene            complement(670..744)
                     /locus_tag="VFB89_08725"
     tRNA            complement(670..744)
                     /locus_tag="VFB89_08725"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(707..709),aa:Asp,seq:gtc)
     gene            complement(792..2498)
                     /locus_tag="VFB89_08730"
     CDS             complement(792..2498)
                     /locus_tag="VFB89_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27429.1"
                     /translation="MSLVRLAVVAAFGFGLAMPLGAQVPRRPREQQVATLPRFMVSNP
                     YVTSAGDSAAAVQVGTGIRDKMTKITDGEFNVLTQDQMNEALKQYGYPPNAILSPSLA
                     STLAKSVQARVLLSSSMSRAGGSQYGVQARLAGVNDEAGSVVTVQQGAAGLGDLGGKV
                     ADQLQPAFKSMPDAKSCIDERSAKPDKAQEAAGKALKQYPGNGLAEYCLAQIALAKKD
                     TKTAVQHFQAAAKNDPLSINSWANLAELYQSQNDTANVLNTFAEMLKVAPTNQKLREQ
                     AFKYFLNANKPDIAKAIADSGIKSDPYNPDFYDLKSNACLFLSDFACAVDALEAGYNV
                     DSTKADTLFFTKITVAAQQKPDTARLLKWAKIGNKKFPQNVTLLKALAKAYTLAGPPD
                     SAMAVTQRLVQADPNDMTPALELAQTMIANKQTKEALPLVQLVAQKGDSQAKENAAAL
                     LYQAAAPMLQEPTQDYVGAADLLRTAVTTANPNGKVYPAANYLLGLSTLLQVPQVDPK
                     AEKGKSCDLAKQESALLDEAEKAFTAGQSANPEVATRNLGIIAKYKPRAASMIKAYCK
                     GK"
     gene            complement(2564..2638)
                     /locus_tag="VFB89_08735"
     tRNA            complement(2564..2638)
                     /locus_tag="VFB89_08735"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2601..2603),aa:Val,seq:tac)
     gene            complement(2710..2901)
                     /locus_tag="VFB89_08740"
     CDS             complement(2710..2901)
                     /locus_tag="VFB89_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27430.1"
                     /translation="MSGPGTREPARRTLLGRRNIVLLSAALLTVIAGYALLQAGNPTM
                     AGVLLVFGYCVLFPVGIAS"
     gene            complement(2935..3732)
                     /locus_tag="VFB89_08745"
     CDS             complement(2935..3732)
                     /locus_tag="VFB89_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27431.1"
                     /translation="MAWSAQLGLATALAREAAQPSVWPGLGSLRPDSVTLIVVPDRAR
                     MDSLVRGQAPKWGAALAVPESHTILLRADAGDVRRTLRHELAHLALHRKVQTRVPLWF
                     DEGYAALAAGEWERLGGLELNLAVARGAVPEFRDLDAALRGTSQAADAAYALAMSAVL
                     ELARRNPSGQLDPLFRLLSHGEGFEAAVLATTGLSLSRFEDAWRRSVRTRYTLVTWLA
                     AGGLWFIVAAVSVVLVWWRRRADRGRRAALDVGWNVVEDPPEPPPAA"
     gene            complement(3829..4704)
                     /locus_tag="VFB89_08750"
     CDS             complement(3829..4704)
                     /locus_tag="VFB89_08750"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM27432.1"
                     /translation="MSETSAMALRAQYREIEQRLDSITGDPERDAVKRDIIALFKRVD
                     GALGELAQLKEDIRTLVDRYKQMSASSDAPQAPQFTGARPAVQADHLNASTYIEKGWS
                     LISLGDYGGAIQALQNALSRSPGATQALSLLGWAQMLSEQYDEALGTFSKVLMKEPAN
                     SLARINVGYICLKKRIFGEAIEHLSRAIRLDNDRKATLYAHYYLGLVYLEREMYEDAQ
                     TFLQKTLALGPNLVEAWFELGRARWFAGDHEGAKQAWIDGIKANKFNPWAKRCQEMLD
                     LAARGAEIPRNTESA"
     gene            complement(4701..5162)
                     /locus_tag="VFB89_08755"
     CDS             complement(4701..5162)
                     /locus_tag="VFB89_08755"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HZM27433.1"
                     /translation="MATEDEIQVVAFRVGPQEFALDILQVERILRYERPSPLPKAPAF
                     LEGVVRYDDAAVPVVDFRKRLELPDPLREETRLMILQLDDQRIAVVVDEVREVMRVDS
                     TTITPPPPVVRGLAAPYIAGIITRGDRTIVLLHALKLLSSTERLALAGASA"
     gene            complement(5166..5600)
                     /locus_tag="VFB89_08760"
     CDS             complement(5166..5600)
                     /locus_tag="VFB89_08760"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HZM27434.1"
                     /translation="MTGGGAAAVLLVRAGRRVVALALDHLVEVVDPGTPITVPARQPA
                     LRGLTSIRGKLVPLVHLGALLDGSGCPEQVSDAGVVIAVGERRICLEVEGVEEVLREP
                     ALPVPPGAEPLPWASGVTRGPDGLVPVLDLPALGGRLAEGGS"
     gene            complement(5597..6685)
                     /gene="cheB"
                     /locus_tag="VFB89_08765"
     CDS             complement(5597..6685)
                     /gene="cheB"
                     /locus_tag="VFB89_08765"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HZM27435.1"
                     /translation="MAAARSEGTVRQPSVLVVDDSPFFRRVIADVVMSTGEFRVIATA
                     RNGMDAIRKVHERLPDLVLMDLEMPELDGLGTIGYLMSEAPRPIVVVSAYAGPGTAAA
                     IRALELGAVDLVPKEDDRTAPALARFSERLLTALRAASAADIHRLPVLARPAQGTPAA
                     PALTLPGRARRCVAIAASTGGPRALADLVPGLERGHEAAIVIAQHMPPKFTRSLAERL
                     AAQSRISVVEAEHGTPLLVDTAYVAPGDWHMRVALGANGPQLELGRDPPVWGVRPAAD
                     PLFRSVADVFGSQSVGVVLTGLGRDGAEGLRAIHDADGVGIAQDRDSATIYGMPNAAI
                     QAGGVDHVLPIARIAERVNRELARLAEL"
     gene            complement(6666..7163)
                     /locus_tag="VFB89_08770"
     CDS             complement(6666..7163)
                     /locus_tag="VFB89_08770"
                     /inference="COORDINATES: protein motif:HMM:NF015908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HZM27436.1"
                     /translation="MSGSNGREVIVRVADLKVGEACDVLVTVGLGSCVAIILHDPAAK
                     VGGMAHVLLPSPGLSRRTENPAKAAQTAVPLLLQQMARRGAQPRRITARIAGGASMFA
                     ALSAPGTINMGERNVVSSRNVLMDHAIPLVAEDIGADYGRTVRFRIADGRVDISSVAH
                     GSRTL"
     gene            complement(7160..7981)
                     /locus_tag="VFB89_08775"
     CDS             complement(7160..7981)
                     /locus_tag="VFB89_08775"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HZM27437.1"
                     /translation="MSSAAEDLAFEALARRISQGGGLAVEAYKTKCIRRRIAVRMRAC
                     GVHTYGEYHALLDRNPAEYDRLRDALTINVTRFFRNAETWERLGNGFLSQLWSPTARL
                     RIWSAGCASGEEAYSLAILAAELAAKRGDPSALERVQIDATDIDRESLRRAGSGRYRP
                     EALLEMPILLRERYFEQVGSEYRVIERIRQRVDVRALDLSRERPPASAYDLIVCRNVV
                     IYFERPMQERLFQTFYETLRPGGLLLLGKVETLFGVARDRLTLLDARERIYRRAG"
     gene            complement(7978..9597)
                     /locus_tag="VFB89_08780"
     CDS             complement(7978..9597)
                     /locus_tag="VFB89_08780"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HZM27438.1"
                     /translation="MAIKGSLKEASLPDVIQLLFLGRRSGCLALADRQNFGMIYFDEG
                     QVAHAAIVNRRDRLGDILTRSKRITAEQLRSAIDAQGKARERKIGEILVEQGAISPDE
                     LQEYVRLQIEEAVYYLFTWSQGTFNFEAGVRPEGEEFPVRINPEYLLLEGARRVDEWS
                     LIQKKIPSFDLIFSADAAHVGQSDVTLSSAQRTILPLLDGSRDVQQIVDESGLVEFEV
                     GQALYGLIQAGFAHRVGTSAAAPAPRVNEARVEEHRNLGIAFYKAGMLDEAMREFRRV
                     ADLRPAEGSAAFYLGLIALRQGRWAEAVDALHSASEKSGPRPATLHDLGIALEQLGRL
                     DDAEKAFGDAAGRAREDARIMLGWAVSALKRGEHTVALGRLARVREILGDKPAPATWY
                     WAASLAHAGADDTETAVSVARAGVTAFPQQAVLRNNLAVLLEGAGDLAGAEAELRAAL
                     AEDPSLPQIFKNLADLCYRSGRYDEAYEAYERAAKLQPDLGDDLYFKLGNLAYKRRDA
                     ARAKASWQRATELNPAHQLARANLDMLERAS"
     gene            complement(9597..>11620)
                     /locus_tag="VFB89_08785"
     CDS             complement(9597..>11620)
                     /locus_tag="VFB89_08785"
                     /inference="COORDINATES: protein motif:HMM:NF012529.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HZM27439.1"
                     /translation="FDTMVVGAANRLAVTAARAVAETPGTVYNPLFVYARPGLGKTHL
                     LMAMGHGAAAIDPSLDVEYLTLDHFIESFHAAIASGQGEAYRKGFLDVGLLLVDDVQF
                     LTGQRETQAELLRIIDAMQTAGRQIVLTSDRPPAEIEALDERLIRRFAGGLVIDIAAP
                     DYETKVAILRRKAEERRATLEPGVLEAVARLPIDNVRELVGALNRLVAHQAAGESVEP
                     EQVPALLGVKARSSANAGGDHSQREPAGAKASEAGADADIETSSSAAAAESADASPRP
                     SPPADEFGDFLSEISATLSQQIESWRGKVAAAILRWGGEGYRTGRLELLLEGELSRDP
                     EEALREFEADVRKLDTLRTEVEKIAPALAGSPLFRDPGDVAAAEALLEQAREGADPPP
                     APSPLWRLDALVESAANRVAVQAAQAVIAHPGQKYNPLVLVGGSGVGKTHLLHAVGNA
                     LIAGGLRTVACVGAEEFTGELIEAIDRDAIAQWRKRYRRVEAFLLDDVHLVAEKGRSQ
                     DELFLLFNRFLESGRQMVFTSAVPLPQLSGVEPRLLTRLEGGLVAELPIPDREIRQRV
                     AERLLSEKLERPDPEVAAYLAGRPADSVRAVHGLVQRVLSAAEAQNAKLSAAFARSVL
                     ETSPVRPARRAAQSRGSGLVAPGIGGARSREKMVWDWPDIGDRLIEEWR"
BASE COUNT         2003 a         3946 c         3667 g         2004 t
ORIGIN      
        1 gtcggaaggg ccggcacggg aacccagcac ggcgcgaatc gcatttacga gatcgtcgct
       61 cgtctcgaac ggcttgccat ttcttcgacg gacgatctcc gccgcaagcc ggcgtgcccg
      121 cggctcgtcg gcccaatccg acagccactc agcgagcgtc gtctgctcgg tgctgttcag
      181 aagatcggcc gcggtatgcc cggtgccttc catccgcatg tcgagctccg caccgggacg
      241 aaagctgaag ccccgctcgg tctgatccag ttgccgggac gagacgccca aatcgagcag
      301 gacgacgttg ggacgaaatg ccgcgaccgc agacagcgcc gcgtccgatc cgaacgacgc
      361 ctcgagatac gtcattcgac tgtcgcccag tcggctcctg gcaaccgcga tggcatcagg
      421 gtctcgatct attccgagaa cctcggcacc ggcttcccga agcgccgcgg cgtgacctcc
      481 gtcgcccagc gtcgcgtcga ccgcacggct cggctgaccg atggcgtcga gcagcgggcc
      541 gaggagaacg gggttgtgga agccagtagc catagccgtt cgccgtcagc cattggcggt
      601 tagcggttgc ggaacctaca acaacacccc gtcccaaacg gaacggggtg ctgatggcaa
      661 cctatcgagc ggggctgacg gggctcgaac ccgcgacctc cgcagtgaca gtgcggcact
      721 ctaaccaagc tgagctacag cccctccgac ggcggggaca atataggcac cccgccacca
      781 cccagaaagc cctacttgcc cttgcagtag gccttgatca tcgacgccgc acgaggcttg
      841 tacttggcga tgataccaag gtttcgggtg gccacttcgg ggttcgcgct ctggcctgcg
      901 gtgaacgcct tttccgcttc atccagcaag gcgctttcct gcttggcaag gtcgcaggac
      961 ttgcccttct cggccttcgg gtccacttgc gggacctgca acagcgtcga aagacccagc
     1021 agataattcg cggccggata caccttgccg ttggggttcg cagtcgtcac tgctgtgcga
     1081 agcagatccg cggcgcccac gtagtcctga gtcggctcct gcagcatcgg cgctgccgcc
     1141 tggtagagga gtgctgccgc gttctccttg gcctgggagt ctcccttctg ggcgaccaac
     1201 tgcaccagcg ggagcgcctc cttcgtctgc ttgttggcga tcatggtctg ggccaactcc
     1261 agcgcaggcg tcatgtcgtt cggatcggcc tgaaccagac gctgagtcac cgccatcgcg
     1321 ctatccggcg gaccagcgag cgtatacgcc ttggcgagcg ccttcagcag ggtcacgttc
     1381 tggggaaact tcttgttccc gatcttggcc cacttgagca gacgcgccgt atcgggcttc
     1441 tgctgagccg ccaccgtgat cttcgtgaag aagagtgtgt cggccttggt cgagtcgacg
     1501 ttgtacccgg cttctagcgc gtcgacggca cacgcgaaat cgctcaggaa gaggcaggcg
     1561 ttgctcttca ggtcatagaa gtcgggattg tacggatcgc tcttgatgcc cgagtccgcg
     1621 atcgccttgg cgatgtcggg cttgttggca ttgaggaagt acttgaacgc ctgttcccga
     1681 agtttctgat tggtcggagc gaccttcagc atttccgcga aggtgttgag cacgttcgcg
     1741 gtatcgttct gggattgata cagctcggcc aggttggccc aggagttgat gctcagagga
     1801 tcgttcttgg ccgccgcctg gaagtgctgc acggctgtct tggtatcctt cttcgccaag
     1861 gcgatctggg cgagacagta ttcagcgaga ccgttgccag gatactgctt caacgccttt
     1921 ccggccgcct cttgagcctt atccggcttg gccgagcgct cgtcgatgca gctcttggca
     1981 tcgggcatgg acttgaaggc aggctgaagc tgatcggcca ccttgccgcc cagatcaccg
     2041 aggcctgccg ctccctgctg gaccgtgacg acgcttcctg cctcgtcatt tacccccgcg
     2101 agccgcgcct gcacaccgta ctgcgatcct ccggctctgc tcatggagga agacagaagg
     2161 acacgcgcct gcacgctttt cgccagggtc gacgccaagg acggactgag gatggcgttg
     2221 ggggggtacc cgtactgttt caacgcctca ttcatctgat cctgggtgag aacgttgaac
     2281 tcgccatcgg tgattttcgt catcttgtcc cgaatccccg tgcccacttg tactgcagca
     2341 gctgagtcgc cggcgctggt gacataggga ttcgagacca tgaaacgcgg cagcgttgcc
     2401 acctgttgct cgcggggacg ccgcggcacc tgagcgccca gcggcatcgc gagcccgaag
     2461 ccgaaggcgg cgacaacggc gagacgaacc agggacatgc aaaacctccg aaacgagtca
     2521 ggtctaccgt tacgacagtc gcccgagagc acgcaagaga ttatgggcga agagggattc
     2581 gaacccccga cttcctgcgt gtaaggcagg cgctctgaac cagctgagct attcgcccgg
     2641 tcgcttgatt ggcctaccca atgtccgacc catcgacgca gggagtatgc cgtgcctggg
     2701 ggctattcgc tatgacgcaa taccaacagg gaagagcacg cagtaaccga acacgagcag
     2761 gacgccggcc atcgtcggat tcccggcctg cagcagggca taacctgcga tgaccgtgag
     2821 cagagccgca cttaggagga cgatgttccg ccgtccgagg agcgtacgcc gggcaggctc
     2881 ccgggttccc ggaccggaca tagacgtgga ggctatcacc cagccggggt caggtcaagc
     2941 tgcgggcggc ggttccggtg ggtcttcgac cacgttccat cccacatcga gggcggctcg
     3001 gcgtccccga tccgcccgcc ggcgccacca gaccaggacg acgctgaccg ccgcgacgat
     3061 gaaccagagg ccacccgccg caagccaggt caccagcgtg tagcgcgtcc ggaccgatcg
     3121 gcgccaggca tcttcgaatc gggacagcga gaggccggtg gtcgccagaa ccgcggcctc
     3181 gaagccttcg ccatgtgaga ggagtctgaa gagcgggtcc agctgtccac tggggttccg
     3241 ccgagccagt tccagcaccg ccgacatggc gagcgcgtag gcggcgtccg cggcctgact
     3301 ggttccgcgg agtgctgcat ccagatcgcg aaattcgggg acggcgccgc gcgccaccgc
     3361 cagattgagc tcgagtccgc ccagtcgttc ccactcaccc gccgccagcg cggcgtaacc
     3421 ctcgtcgaac cagagcggca cccgagtctg aacttttcgg tggagggcaa gatgggccag
     3481 ctcgtgccgg agggttcgcc ttacatcacc cgcgtcggcc cggagaagaa tggtgtgcga
     3541 ctcgggcacc gcgagcgccg ccccccattt cggcgcctgg cctcgtacca gcgagtccat
     3601 tcgggctcga tcaggcacga cgatcagcgt gacgctgtcc ggtcgcaggc tgcccagccc
     3661 cggccacacc gagggttgcg ctgcttcccg ggcgagggca gtcgccagcc ccagttgcgc
     3721 actccaggcc acggcggtga ccggaccgac tcgaatgact tggggaccgg actgctgaag
     3781 cgcgaggcag gaaaggaaaa caatgactgg aacgataccg cgagctccct acgccgactc
     3841 ggtgttgcgc gggatctcgg ccccccgcgc cgcaaggtcc agcatctcct gacagcgttt
     3901 ggcccaggga ttgaatttgt tggccttgat cccgtcgatc catgcctgtt tcgccccttc
     3961 gtgatctcct gcgaaccagc gggcacggcc cagctcgaac caagcctcga ccagattggg
     4021 gccgagcgcg agcgttttct gcagaaaggt ctgagcgtcc tcgtacatct cgcgctcgag
     4081 atacacgagg ccgaggtagt agtgggcgta gagcgtcgct ttgcgatcgt tgtcgagacg
     4141 aatcgctcgg gagaggtgct cgatcgcctc accgaaaatt cgcttcttga ggcagatgta
     4201 ccccacgttg atccgggcga gcgagttggc cggctccttc atcagcacct tggagaaagt
     4261 gcccaaagct tcatcgtact gctcgctcag catctgggcc cagcccagga gcgagagcgc
     4321 ctgagtggct ccgggtgaac ggctgagtgc gttctgcagc gcctggatgg ccccaccgta
     4381 gtcacccagc gagatgagac tccacccctt ctcgatgtaa gtggaagcat tcaagtgatc
     4441 cgcttgcact gccgggcggg cgccggtgaa ctgaggggcc tgcggcgcat ccgacgaggc
     4501 gctcatctgc ttgtacctat cgaccaacgt gcgaatgtcc tccttgagct gcgccagctc
     4561 acccagggca ccgtccaccc gcttgaacag cgcgatgata tcccgcttca cggcgtcccg
     4621 ctcgggatcg ccggtgatcg aatccaggcg ctgctcgatc tcgcggtact gcgcgcgcag
     4681 ggccatggcc gaggtctcgc tcatgccgat gcgcctgcca gcgccagccg ctccgtcgag
     4741 ctgagcagct tgagggcgtg cagcaacacg atggtacgat cccctcgagt aatgattccc
     4801 gcgatgtacg gtgccgccag gccccgcacc accggcgggg gcggcgtaat tgtcgtgctg
     4861 tcgacccgca tcacctcgcg gacctcgtcg accacgacgg cgattcgctg atcatccagt
     4921 tgcaggatca tgagccgcgt ttcttctctc aacggatcgg gaagctccag ccgcttgcgg
     4981 aagtccacga ccgggaccgc cgcatcgtca tagcgcacca cgccctcgag gaaggccggt
     5041 gccttgggca acggcgacgg gcgctcgtag cggagaatgc gctccacttg caggatgtcg
     5101 agcgcgaact cctgaggtcc tacccggaac gcaacgacct ggatctcgtc ttcagtggcc
     5161 acccgtcagc ttcctccttc tgcgagccgg ccgccgaggg ccggaaggtc caggaccggc
     5221 acgaggccgt cgggtccgcg ggtcactccc gacgcccacg gaaggggttc ggcgcccgga
     5281 ggaaccggca gggcaggctc tcgaagcact tcttctactc cttccacctc caggcagatc
     5341 cgccgctcac cgacggcgat gacgacccct gcgtcgctca cctgttcggg gcaaccactg
     5401 ccgtccagca atgcgccgag gtggaccagc ggcaccagct tcccgcggat actggtcaac
     5461 ccgcgcagcg ccggctgacg tgccggaacg gtgatgggtg tgcccggatc gaccacctcg
     5521 acgaggtgat ccagcgccag cgccacgacc cggcggcctg cccgcaccaa aagcaccgct
     5581 gctgctccgc cgccggtcat agttccgcca gtctcgccag ctcgcggttc actctctccg
     5641 cgatgcgtgc aatgggcagg acgtggtcca ctcccccagc ctggatcgcc gcattgggca
     5701 ttccatagat ggtcgcgctg tcccggtcct gtgcgatgcc gacaccgtcg gcatcgtgga
     5761 tggcgcgcag gccctcggct ccgtcccggc ccagccctgt gaggaccaca cctacggact
     5821 gagacccgaa cacatcagca acggaacgaa agagcggatc ggccgccggg cgcacacccc
     5881 acaccggtgg gtcccgaccc aattcgagct gcgggccgtt cgcccccagc gcgactcgca
     5941 tgtgccagtc acccggcgct acatatgccg tatcgaccag cagcggcgtt ccatgctccg
     6001 cctcgaccac cgagatccga ctctgggcgg cgagccgctc cgccagactg cgggtgaact
     6061 tgggcggcat gtgctgcgca atgacgatag ccgcttcgtg gccccgttcg agccccggca
     6121 ccaggtcggc cagggcgcgc ggccctccgg tcgaggcggc gatagcaacg caccggcggg
     6181 cccgtccggg gagcgtcaac gccggcgctg cgggcgtgcc ctgtgcgggt cgggcaagca
     6241 cgggcagccg gtgaatgtcc gccgcgcttg ccgcccggag cgccgtcagc aatcgctcgc
     6301 tgaaacgcgc gagcgccggg gccgtccgat cgtcttcctt ggggaccaga tcgaccgcgc
     6361 ccagctccag cgctcgaatc gcagccgccg tgccaggtcc cgcataggca ctcacgacca
     6421 cgatgggccg aggcgcctcc gacatcaggt aaccgatcgt gcccagtccg tcgagctccg
     6481 gcatctcgag gtccatgagg accaggtcgg gcagtcgttc gtgcactttc cgaatggcgt
     6541 ccatgccgtt ccgagcggtt gcgatgaccc gaaactcacc cgtgctcatc accacatccg
     6601 cgatgactcg gcggaagaat ggactgtcat ccaccaccag cacgcttggc tggcggacgg
     6661 tgccctcaga gcgtgcggct gccatgggcc accgatgaga tgtccacccg tccgtcggca
     6721 atgcgaaacc gcacagtccg tccgtaatcg gctccaatgt cttcggcgac gagcggaatc
     6781 gcgtgatcca tcagcacgtt gcgcgacgac acgacgttcc gctcgcccat attgatcgtg
     6841 cccggcgccg agagcgcggc gaacatgctc gcgcctccag cgattcgggc cgtaattcgt
     6901 cgaggctgag cacctcgacg agccatctgc tggagcagaa gaggcaccgc cgtctgcgcc
     6961 gccttggccg ggttttctgt gcggcgcgag aggcctggcg acgggagaag aacatgggcc
     7021 atccctccga ctttggcggc cgggtcgtgg aggatgatcg cgacgcagct gccaagccca
     7081 acggtaacca gtacgtcgca ggcttcaccg accttgagat cggcgacccg aacgatcacc
     7141 tcccggccgt tcgacccact catccggccc gccggtagat acgttcccgc gcatcgagca
     7201 gggtaagccg gtcgcgcgcc acgccgaaga gcgtctcgac ttttcccagc agcagcaacc
     7261 cgccgggccg cagggtctcg tagaaggtct gaaacaggcg ctcctgcatt ggccgttcga
     7321 agtagatcac gacgttgcgg cagacgatca gatcgtaggc gctcgccggc ggtctttccc
     7381 gacttagatc aagcgcccgc acatccacgc gttgccgaat gcgttcgatg acccggtact
     7441 cgctcccgac ctgctcgaaa taccgctcgc ggagcaagat cggcatctcc agcaaggctt
     7501 cgggccgata ccggcctgat ccggcacgtc ggaggctttc ccgatcgatg tcagtggcat
     7561 cgatttgcac ccgctcgagc gccgatggat ctccccgctt cgctgcgagc tcggccgcga
     7621 ggatcgccag gctatacgcc tcctcgccgg aagcacaccc cgcgctccag atccgcaggc
     7681 gtgccgtggg actccagagc tgggacagga agccgttgcc gagccgttcc caggtctcgg
     7741 cgttccggaa gaaccgagtg acgttgatcg tgagcgcgtc acgaagccga tcgtactcgg
     7801 cagggtttcg gtccagcagc gcgtggtact cgccgtaggt gtggacgccg caagcccgca
     7861 tccgaacggc gatccgccgg cgaatgcatt tcgtcttgta ggcttctacg gcgaggcccc
     7921 cgccctgtga aatccggcgg gctaacgcct cgaacgcgag atcctcggcg gcgctgctca
     7981 cgaggcccgc tccagcatgt cgaggttggc ccgcgcaagc tgatgcgcgg gattcagctc
     8041 ggttgcgcgc tgccagctcg ccttggcccg agccgcgtct cgccgcttgt acgccaaatt
     8101 gcccagcttg aagtagaggt cgtcccccag gtcgggctgg agcttggcag ctcgctcgta
     8161 cgcctcgtac gcctcgtcat atcggccgct ccgataacac agatcggcca ggttcttgaa
     8221 gatctgaggc agcgacggat cttcggcgag agccgcgcga agttcagcct ctgctcccgc
     8281 caggtcgccc gcgccctcga gcaggaccgc cagattgttg cggagcacgg cctgctgcgg
     8341 gaacgccgtg acaccggcac gcgcgacgct caccgccgtc tcggtatcat cagcgccggc
     8401 atgggccagc gaggccgccc agtaccaggt cgcgggcgcc ggcttgtcgc ccaggatttc
     8461 tcgaacacgc gcgagccggc ccagcgccac cgtatgctct ccgcgtttga gcgccgagac
     8521 ggcccagccg agcatgatgc gcgcatcttc ccgggcccgg cccgcggcgt ctccgaacgc
     8581 cttctctgca tcatccaggc gacccagctg ttccagcgcg attccgagat cgtgcagggt
     8641 cgctggacga ggcccgctct tttccgaagc ggaatgaagt gcgtctaccg cttccgccca
     8701 gcgaccctgt cgcaaggcaa tcaggccaag gtagaacgcc gcgcttccct cggcagggcg
     8761 caggtctgcc acccgccgga actcccgcat cgcctcgtcg agcatgcctg ccttgtagaa
     8821 agcgatgccg agattacgat gctcctccac ccgagcctcg ttgacgcgcg gcgctggcgc
     8881 tgcggccgag gtgcccaccc gatgggcaaa ccccgcctga atgaggccgt acagcgcctg
     8941 ccccacctcg aactcgacca ggcccgactc gtccacgatc tgctgcacgt ccctcgagcc
     9001 atcgagcagc ggaaggatgg tccgctgcgc cgaggaaagg gtcacgtcgg actggcccac
     9061 gtgggcggca tccgccgaga agatgagatc gaaggaggga atctttttct gaatcagact
     9121 ccactcgtcc acccgacgtg cgccttcgag caacagatat tccgggttga ttcgaaccgg
     9181 gaactcctct ccttcgggcc ggactccggc ctcgaagttg aaagtcccct gcgaccaggt
     9241 gaacaggtag tacaccgcct cttcgatctg gaggcgcacg tattcctgca gctcgtccgg
     9301 gctgatggcg ccctgctcga ccaggatttc gccgattttt ctttcgcgag ccttgccctg
     9361 agcgtcgatg gcagaccgga gctgttcggc tgtgatgcgt ttgctgcggg tcaggatatc
     9421 gcccagacga tcacggcggt tcacgatggc ggcgtgcgcc acctgaccct cgtcgaagta
     9481 gatcatccca aagttctgac ggtccgcgag cgcaaggcag ccggaccgcc gcccgagaaa
     9541 gagcagctgg atgacatcgg gcaggctcgc ctccttgagg cttcccttga tcgccatcag
     9601 cgccactcct cgatgagccg gtcaccgatg tccggccagt cccataccat cttctcccgg
     9661 cttcgggcgc ctccgatccc gggtgccacc aagcccgacc cgcgggactg cgctgcccgg
     9721 cgtgccggcc gcaccggtga cgtctccagc acggagcgag cgaaagcggc cgaaagcttg
     9781 gcgttctgcg cctcggcggc gctcagtacg cgctgaacca gaccgtgtac ggcgcggacc
     9841 gaatcagcgg ggcgccccgc gaggtaggcg gcaacctcgg ggtcaggccg ctcgagtttt
     9901 tcactcagca gccgctcggc cacgcgctgt cggatctcgc gatccggtat cggtagctcc
     9961 gccaccagcc cgccttccaa ccgggtgagg agacggggct cgacgcccga gagctggggg
    10021 agcggaacgg ccgaggtgaa gaccatctgg cggccgctct cgaggaatcg attgaacaga
    10081 agaaacagct cgtcctggga gcgtcctttc tccgcgacga ggtgcacatc atccagcaga
    10141 aacgcttcga cccgccggta acgcttgcgc cactgagcga tagcatcgcg atcgatagcc
    10201 tcgatcagct cgccggtgaa ctcctcggcg ccgacgcagg ccacggtgcg aagtccgccg
    10261 gcaatcagtg cgttgcctac cgcgtgcagc aggtgggtct tgcctacgcc gctgccaccc
    10321 acgagaacga gcgggttgta cttttgcccc ggatgtgcaa tgaccgcctg ggccgcctga
    10381 acggcgacgc ggttggcggc gctttcgacc aatgcgtcaa ggcgccaaag cggtgagggc
    10441 gctggcggtg ggtccgctcc ctcccttgcc tgctcgagga gcgcttccgc agccgcaaca
    10501 tcgcccggat cgcgaaacag aggcgagccg gcaagtgcgg gcgcaatttt ctcgacttcg
    10561 gtgcgaagtg tgtcgagctt gcgcacgtcg gcctcgaact cacgaagcgc ctcttccgga
    10621 tcgcgagaga gctcgccctc gagcagtagc tcgagtcgcc cggtgcgata gccctcaccg
    10681 ccccagcgca gaatggccgc ggcgaccttg cctctccaac tctcgatctg ctggctcagg
    10741 gtggcggaga tctcggacag gaagtcaccg aactcgtcgg cgggaggaga tggacgcggg
    10801 ctggcgtcgg cagactctgc tgccgcagcc gagctcgacg tttcgatgtc ggcatccgcg
    10861 ccggcttctg aggctttcgc gcccgcaggc tcgcgctggc tgtggtctcc tccagcgttt
    10921 gcgctcgatc gcgccttgac accgagcaac gccggcacct gctcgggctc gaccgactcg
    10981 ccggcagcct ggtgcgcaac cagtcgattg agtgcgccta ccagctctcg cacattgtcg
    11041 atcggaagcc tggcgacggc ctccaggacc ccaggctcga gcgtggcccg tcgctcctcc
    11101 gctttacggc ggagaatggc caccttggtt tcgtagtccg gcgcggcaat gtcgatcacc
    11161 agaccgccgg cgaagcgccg gatcagccgt tcatccaggg cttcgatctc tgccggcgga
    11221 cggtcactgg tgagcacgat ctggcgcccg gcagtctgca tggcatcgat gatccggagc
    11281 agctcggcct gtgtctctcg ctggccggtg aggaactgca cgtcgtcgac cagcaggagg
    11341 cccacgtcga ggaagccctt tcgataggct tccccctgcc ccgacgcgat ggccgcgtgg
    11401 aacgattcga tgaagtggtc cagggtgaga tactcgacgt cgagcgacgg atcgatcgcg
    11461 gcggcgccgt gccccattgc catcagcaga tgggtctttc ctaggccggg tcgggcatat
    11521 acgaagagcg ggttgtacac ggtacctggt gtctcggcca ccgcccgtgc agccgtgacc
    11581 gccaggcggt tggcggcacc caccaccatc gtgtcgaacc
//
LOCUS       DASRJQ010000391         3641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2171749, whole genome shotgun sequence.
ACCESSION   DASRJQ010000391 DASRJQ010000000
VERSION     DASRJQ010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2171749"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="VFB89_08790"
     CDS             <1..832
                     /locus_tag="VFB89_08790"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HZM27440.1"
                     /translation="VLLKHARDAFRDAATVDAAWRGLNYLAPPDPGRAAAEYDRFAAL
                     IEDMGARIHWMPPDDVTSLDSLYVRDASIVSSEGIILCNMGKAARSTEPAAHAEAFRK
                     LGIPVAGAVTGEGKIEGGDVVWLDQATLAVGRGYRTNDEGIRQLRGILGEGIDVVVAY
                     LPHWRGPADVFHLMSILSPIDRDLALVYSPLLPVAFRELLLSRGFDLVEVPEAEFESM
                     GCNVLALGPRRCLMLDGNPVTQRRLEKAGAQVAVFSGEEICRKGSGGPTCLARPIMRS
                     "
     gene            complement(886..1254)
                     /locus_tag="VFB89_08795"
     CDS             complement(886..1254)
                     /locus_tag="VFB89_08795"
                     /inference="COORDINATES: protein motif:HMM:NF022317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HZM27441.1"
                     /translation="MSFRILADITVLAHLTFVLFVVLGGFLAARWPRVAWVHLPAAAW
                     GVWVELAGWSCPLTPLENWLRRQGGGFAYRTSFAEHYLIPLLYPGSLSRDVQYGLACL
                     VFLVNAIIYAVLLRRWARRS"
     gene            complement(1310..2152)
                     /locus_tag="VFB89_08800"
     CDS             complement(1310..2152)
                     /locus_tag="VFB89_08800"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZM27442.1"
                     /translation="MRWLVTVLTAALVSAGIGHAPPDQARPDSPLVYKTPGMDQVRVK
                     RDLTYKKLASTEFQVDLFLPAAQGKQAPVVMLISGNSQPRKVFLGMEQMGRVLAANGI
                     AAVLFDKRYQRAGYYDAEQDMTDLTAFLHREADELGLDMSRVCHFGYSGGGPLLSIAL
                     RPGSTARCVVGYYPMMDSDVWGAPADSRRDQAMRDFSPVQQIAAGKGKTPPVFLVRAG
                     IDNPELLATIERFVRESIAQNADLTFLNYPQGHHAFDIRDDNDQSRAVIKATVEFLER
                     QLNR"
     gene            complement(2460..2876)
                     /locus_tag="VFB89_08805"
     CDS             complement(2460..2876)
                     /locus_tag="VFB89_08805"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZM27443.1"
                     /translation="MSKKQELIDGLNDDLNLELEAVLRHVYHAATATGLLGHELREIL
                     KADLQGELAHAAFLADKIAALEGEVQIDVKMPKKLKRAKEMLEAEVAAERKLVTSYAQ
                     RIKQAEEAGDPGLVIHLEGILSEETDHAEELERLAR"
     gene            complement(3082..3285)
                     /locus_tag="VFB89_08810"
     CDS             complement(3082..3285)
                     /locus_tag="VFB89_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27444.1"
                     /translation="MPVTRYRSVEEMPRPWREANSPGNLRLVARMLAVYRGFAGDTPR
                     QAGVHRFRSLQEANASRSDPYRR"
     gene            complement(3287..>3641)
                     /locus_tag="VFB89_08815"
     CDS             complement(3287..>3641)
                     /locus_tag="VFB89_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27445.1"
                     /translation="DLDLFIKADRENVERLKRALRSVFPDPSIDEISAEDLCGDYPAV
                     RYLPPDGFGFDLLTRLGTAFQYEDLEIEEKNYDGVTVRVVTPRTLWRMKKDTVRPSDR
                     FDAQVLAERFGFREE"
BASE COUNT          651 a         1157 c         1159 g          674 t
ORIGIN      
        1 cgtcctgctg aagcatgcac gcgatgcctt tcgcgacgcg gcgacggtgg acgcggcgtg
       61 gcgcggactg aactatctgg cgccgccaga tcctggccgc gccgccgcag agtacgaccg
      121 cttcgcggcg ctcatcgagg acatgggggc ccgcatccac tggatgccgc ccgacgacgt
      181 caccagcctt gactccctgt acgtgcgcga cgcctccatc gtctcgagcg agggcataat
      241 cctgtgcaac atgggcaagg cggcgcgctc gacggagccg gcggcccacg ccgaggcctt
      301 ccgcaagctc gggatccccg tggccggcgc cgtcacaggc gaggggaaga tcgaaggagg
      361 ggacgtcgtc tggctcgacc aggcgacgct ggcagtgggg cgcggctacc gcaccaacga
      421 cgagggcatc cgccagctac gggggatcct cggcgagggg atcgacgtcg tcgtggccta
      481 tctgccccac tggcgcggcc ccgccgacgt cttccatctg atgtcgatcc tcagcccgat
      541 cgatcgcgac ctcgcgctgg tctactctcc cctgctgccg gtcgcgttcc gggagctgct
      601 gctctcgcga ggattcgacc tcgtcgaggt tccagaagct gagttcgaga gcatgggatg
      661 caatgtcctg gccctcggcc cgcgccgttg cctcatgctc gacggcaacc ccgtcacaca
      721 aaggcgcctg gagaaggcgg gagcgcaggt cgcggtcttc tccggcgagg agatctgcag
      781 gaagggatcg ggaggcccca cctgcctcgc ccggccgatc atgagatctt gaaccgcaag
      841 cgctccccgg tcatggagac attctggtga tgcgctcagt tccagctacg agcgccgggc
      901 ccaacggcga agcaggacgg cgtagatgat cgcattgacc agaaagacga ggcacgccag
      961 gccgtactgc acatctcgcg agagcgagcc ggggtagagg agaggtatca gatagtgctc
     1021 ggcgaagctc gttcggtagg cgaagccgcc cccttgccgg cgcagccagt tctcgagcgg
     1081 tgtgagaggg caggaccagc cggccaactc gacccacacg ccccaggctg cggccggcag
     1141 atggacccat gccaccctgg gccatcgtgc ggcgaggaac ccgccgagca ctacgaacag
     1201 cacaaaggta aggtgcgcga gcaccgtgat gtcggctagg atgcggaagc tcattccgac
     1261 accacagtag ctcaattttg agcgcctgat ctgctatgcg ccacctggct cagcggttca
     1321 actgtcgctc gaggaactcg actgtcgcct tgatgaccgc gcgtgattga tcgttgtcgt
     1381 cgcggatgtc gaatgcgtga tggccctggg ggtagttcag gaaggtcagg tcggcgttct
     1441 gggcgatcga ctcgcgcacg aatctctcga tggtcgcgag caattcggga ttgtcgattc
     1501 cggccctcac aaggaagacg ggaggagtct tcccctttcc ggccgcgatc tgctgcactg
     1561 gggaaaagtc cctcatcgcc tggtcacgac ggctgtccgc cggggcgccc cacacatccg
     1621 aatccatcat ggggtagtac cccaccacac agcgcgccgt gctgccgggc cgtagcgcga
     1681 tgctgagcag cgggccgcca ccagaatagc caaagtggca aacgcggctc atgtcgagcc
     1741 ccaactcgtc ggcctctcta tggaggaatg ccgtcaggtc ggtcatgtcc tgctcggcgt
     1801 cgtagtaccc cgcacgttgg taccgcttgt cgaagagcac tgccgcgatt ccattggcgg
     1861 ccaggacgcg tcccatctgc tccattccca gaaagacctt gcgcggctgg gaattcccgc
     1921 tgatcaacat cacgaccggc gcttgcttgc cctgggctgc aggcaggaac aggtcgacct
     1981 ggaactccgt gcttgcgagc ttcttgtagg tcaagtctct cttgacgcgg acctggtcca
     2041 tgccaggggt tttgtagacg agaggagaat cggggcgggc ctggtccggc ggcgcatgac
     2101 caatcccggc agagacaaga gcggcagtca gcaccgtcac cagccatcgc atggaacgcc
     2161 ctccgtcagg aggtgccatg gccggtgaca ctccgagtgg cttcagtcta gctcagtgcc
     2221 gggcagctcc gcctgcagtc agagcaacag gccgtcgcca tggcttcaag gctggtcggc
     2281 aggccaggga acccaatccc gaattttgag aatgtagaga tcgagccccg cggccatgaa
     2341 gttgaaaacg agcagagcgg aggagaatcg tgtcgttccc atgatgcttc cctcctcgag
     2401 gatggcgagg tgctgcgttt ggcttgatta tggttccgat acggtaggat cctctcccct
     2461 taccgggcca agcgctcgag ttcctcggca tggtctgttt cctccgagag aatcccctcg
     2521 agatggatga ccagccccgg gtctccggcc tcttccgcct gcttgatgcg ctgagcgtag
     2581 ctggtcacca gctttcgctc cgcggctacc tctgcttcga gcatctcctt ggctctcttg
     2641 agcttctttg gcatcttcac atcgatctgg acttcgccct ccaatgcggc gatcttgtcg
     2701 gcgagaaaag ccgcatgggc gagctcgccc tggaggtccg ccttcaggat ctcgcgcagt
     2761 tcgtgtccga gcagtccggt ggcggtcgcg gcgtgataca cgtggcgcag cactgcctcg
     2821 agctccaggt tcagatcatc attcagtccg tcaatgagtt cctgcttctt cgacatgaga
     2881 cgtcctccgt cgattcgacg cgcccacgat gaacggccac gatcccgagc catagagcgc
     2941 catcagggtc aggattatac gtcaaccggt gagcgagccg gcgcgctcgc actgggtgcg
     3001 cgtgatcgag gcactgaagc gcgaggggta cgacgcggcc gcgctgatcg cgaagcacga
     3061 gtcggaagtc gaacccgtgc ctcaccggcg gtaggggtca ctccggctgg cgttggcttc
     3121 ttgcagtgac ctgaaacggt gaacgccggc ctggcggggt gtgtctcccg caaagcccct
     3181 atagacggcc agcatgcggg ccacgagtcg aagatttccc gggctgtttg cttcccgcca
     3241 gggtctgggc atctcctcga cggagcgata gcgagtgact ggcatgctac tcctcccgga
     3301 agccgaatct ctcggccaga acctgggcat cgaagcggtc ggacgggcga acggtgtcct
     3361 tcttcattcg ccagagagtc cttggcgtga ccaccctcac ggtgacgccg tcgtagtttt
     3421 tctcttctat ctcgaggtcc tcgtactgga aggcggttcc cagtcgcgtc aggagatcga
     3481 agccgaatcc atcgggcggc aggtaccgga ccgccggata gtctccgcac aaatcctcgg
     3541 cggatatctc gtcgatgctc gggtccggga agaccgatcg cagagctcgt ttgaggcgct
     3601 ccacgttttc ccgatcggct ttgatgaaaa gatcgagatc a
//
LOCUS       DASRJQ010000392         3967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3047049, whole genome shotgun sequence.
ACCESSION   DASRJQ010000392 DASRJQ010000000
VERSION     DASRJQ010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3047049"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VFB89_08820"
     CDS             <1..630
                     /locus_tag="VFB89_08820"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZM27446.1"
                     /translation="SPAPGSWAILWDDKFRGRISLQDDIATLYMVAQLLGLDDPRDPA
                     KLYNLSDADLAKVKAKMLELRPRVRKYWATAGDLTQLFQAGEVVLAEGWPLMTHQLRQ
                     AKFPAAEVIPKEGTTAWADHWVLTSGAKNLDAAYAWLEYAAQPFTQKLLYDVTRYIVA
                     HPGAKAYMTPEEATSQHDIAEYGARVNFWQWGSRRDKYQEIWNEVKAAQ"
     gene            631..1710
                     /locus_tag="VFB89_08825"
     CDS             631..1710
                     /locus_tag="VFB89_08825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZM27447.1"
                     /translation="MSEPLLRLVDVSKRFGDVTAVDGVSLDIAAGEFLTILGASGSGK
                     TTTLRLIAGFEPPTAGQILMNGADIAPLPPYRRDVNTVFQQYALFPHLDVRENVGYGL
                     RMRGVPPPECESRVAEALAMVRLGDLGPRKPRQLSGGQQQRVALARALVNRPQLLLLD
                     EPLGALDLKLRRAMQLELKHLQTRLGITFVYVTHDQEEALTMSDRIALMRSGRIEQLG
                     KPDEIYHRPRTRYVADFIGETNLLGCKVLATAAEVVTLDLNGSVLRAPVGWGASIPAG
                     AVALLSVRPEQLVIGPRGDVGPERNRLDGVVADVVFVGAGSKVHVALGNGTRMVVNWA
                     GDATFAPGIPVSVTWPVEVGVLLRD"
     gene            1703..2569
                     /locus_tag="VFB89_08830"
     CDS             1703..2569
                     /locus_tag="VFB89_08830"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM27448.1"
                     /translation="MISSQVAARGRVLRLGALLPLAWAVVLLFVPYAFLLVQSFWRVA
                     GGALTPDFTLDNYRRLIGTSLYPRTILYSAETALWVTGFALLLAYPLAYLLAFKARRH
                     RELLYFAVIIPLWVSYVVRAYAWKIILGQHGILNGLLLSLGVIHEPLSVLLYSRWAVV
                     VALTHIYTPFALMPIFAVLESIPPSLREASQDLYAGRWRTFLHVTLPLSLPGVLAGAT
                     FAFVLSMGDFLAPQLLGGNDSALMVSNLVWSLFGVAYNWPLGAAVAVVMLGLTLALLA
                     LTGAIERRGQTR"
     gene            2566..3366
                     /locus_tag="VFB89_08835"
     CDS             2566..3366
                     /locus_tag="VFB89_08835"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM27449.1"
                     /translation="MRRGSDYPLWIAGVGALAFLYLPVAVVVAFSFHDQSIIAWPLQL
                     GTLRWYGMLAADTGMLAAAWASVKLALLAVAIALVMGVPGAFVLDRLDFPGKTAFRRL
                     VLLPLILPGVITGVALLSFFSLLHLSLSSGYPLVPGWPVVLGHGAALTPVVTTQVFAR
                     LQRLDRSLEEASQDLYADEWRTFRYVTLPTIRSAVLGSALLVFTLSLDEIAVTFFLVG
                     RQNTLPLQLWGLLRRGITPEVNAAATVIFVVSVAVIVLAARAMQQNPD"
     gene            3645..>3967
                     /locus_tag="VFB89_08840"
     CDS             3645..>3967
                     /locus_tag="VFB89_08840"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZM27450.1"
                     /translation="MSPQPLVLLLAATLAGVPLGEVHAQQPFALTGRITNESGNPVPN
                     ATVAVPALEIGTTVRSDGSYTLALPDRARGQTVTLSVRAIGYKPFAVQVAVGETGATQ
                     DFKLE"
BASE COUNT          589 a         1324 c         1296 g          758 t
ORIGIN      
        1 tcgcccgcac cgggttcatg ggcgattctc tgggacgaca agttccgggg ccggatctcg
       61 ctgcaagacg acatcgccac cttgtatatg gtggcccagc tgctcgggct cgatgatccc
      121 agggatccgg ccaagctgta caatctgtcg gacgccgatc tcgcgaaggt gaaggccaag
      181 atgctggagc ttcggccgcg agtacgcaag tactgggcga cggcgggcga cctgacccag
      241 ttgttccagg ctggcgaggt cgtgctggcc gaggggtggc ccctcatgac tcatcagctg
      301 cggcaggcca agttccccgc cgccgaggtg attccaaagg aaggaaccac ggcttgggcc
      361 gaccattggg tgctgacctc cggagcgaag aacttggacg ccgcgtatgc ctggctcgag
      421 tacgccgcgc agccgtttac ccagaagctc ctctacgatg tgacccgata catcgtcgct
      481 cacccggggg ccaaggcgta tatgacgccg gaggaggcga cgagccagca cgacatcgcg
      541 gagtatgggg cccgggtaaa cttctggcag tggggcagcc ggcgcgacaa ataccaggag
      601 atctggaacg aagtgaaagc ggcgcagtaa gtgtccgagc cgctgctccg tctggtcgat
      661 gtcagcaagc ggttcggcga cgtcaccgcc gtcgatgggg tatcgctcga catcgccgcc
      721 ggcgagttcc tcacgatcct gggcgcctcc ggctcgggga agaccaccac cctccggctc
      781 atcgccggct tcgagccacc caccgccggg cagatcctga tgaacggcgc cgacatcgcg
      841 ccgctgccgc cctatcgccg cgacgtcaac actgtcttcc agcagtacgc cctcttccct
      901 cacctcgatg tccgcgagaa cgtcgggtac gggcttcgga tgcgcggggt cccgccaccg
      961 gaatgcgaga gtcgggtagc cgaggctctg gcgatggtgc ggctgggaga tctgggcccg
     1021 cggaagccgc gccagctttc cggcggccag cagcaacggg tggcgctcgc tcgggcgctg
     1081 gtcaaccgtc cccagctcct gctgctcgat gagcctctgg gtgcgctcga tctcaagctc
     1141 cggcgcgcga tgcagctcga gctcaagcac ctccagaccc gtctcggcat caccttcgtg
     1201 tacgtgaccc acgatcagga agaagcgctc acgatgagcg atcgcatcgc gttgatgcgg
     1261 agcggtcgaa tcgagcagtt ggggaagccg gatgagatct atcaccggcc caggacccga
     1321 tacgtcgccg atttcatcgg ggagaccaat cttctgggtt gcaaggtgct ggcgacggcc
     1381 gccgaggttg tcacgctcga tctgaatggc tccgtgctgc gcgcgcccgt tgggtggggc
     1441 gcctcgattc cggccggcgc ggttgccttg ctgtcggtcc gcccagagca gcttgtgatc
     1501 ggcccgcgag gtgacgtggg gccggagcga aacaggctcg acggcgtcgt ggccgacgtc
     1561 gtgttcgtgg gcgccggcag caaggttcac gtagcgctgg ggaacggcac ccgaatggtg
     1621 gtgaactggg ctggcgatgc cacgtttgcg ccgggcattc ccgtcagcgt cacttggccg
     1681 gtagaggtgg gagtgctgct gcgtgattag ctctcaagtg gcggcgcggg gtcgagtgct
     1741 gcgcctgggt gcgctgcttc ctctcgcgtg ggccgtggtg cttctcttcg tgccctacgc
     1801 attcctcctg gtccaatcct tctggcgagt agcaggcggc gcgctcacgc ccgattttac
     1861 gctagataac taccggcgat tgatcggcac gtcgctgtat ccccggacca ttctctattc
     1921 cgccgagact gcgctgtggg tgaccgggtt cgcgctgttg ctggcctacc cgctggccta
     1981 cctgctcgcc tttaaggccc gccgccaccg cgagttgctc tacttcgccg tcatcatccc
     2041 gctctgggtg agctacgtgg tgcgggccta cgcgtggaag atcatcctcg ggcagcacgg
     2101 gatcctgaat ggcctcctgc tctcgctcgg cgtgattcac gagccacttt ccgttttgct
     2161 ctatagccgg tgggcggtcg tggtagcgct gacccacatc tacacgccct tcgctctcat
     2221 gcccatcttc gcagtgctgg agtcgatccc accatccttg agggaagcga gccaggatct
     2281 ctacgccggg cggtggcgta ccttcctcca cgtcacgttg ccactgtcgc tgccgggcgt
     2341 gctcgccggg gccacgttcg ctttcgtgct cagcatgggc gacttcctcg ccccgcagct
     2401 cctcggcggc aacgacagcg cgctgatggt atccaacctc gtgtggagcc tgttcggcgt
     2461 cgcctacaac tggccactcg gggcggcggt cgcggtcgtc atgctggggc tgaccctcgc
     2521 gctcttggcg cttaccggcg caatcgagcg gcgggggcaa acccgatgag gcggggcagc
     2581 gactatccac tctggatcgc gggtgttggc gcgctcgcct tcttatatct ccccgtcgcc
     2641 gtggtagtgg cattctcctt tcacgaccag agcatcatcg cctggcccct tcagctgggg
     2701 acgctccgct ggtatgggat gctcgccgcc gataccggca tgctggcggc ggcctgggcc
     2761 agcgtaaagc tcgccctgct cgcggtcgcg atcgcgctgg tcatgggtgt tccgggcgcg
     2821 tttgtcctcg atcgactcga ctttcccggc aagacggcct tccggcggct ggtgttgctc
     2881 ccgctcattc tccccggcgt catcaccggc gtcgcgttgc tctcgttctt cagcctgctc
     2941 cacctcagcc tgtcgagcgg atatcccttg gtgcccgggt ggccagtcgt gctgggtcac
     3001 ggggcggcgc tcacaccggt ggtgaccact caggtgttcg cgcggctgca gcggctcgac
     3061 cggagtttgg aagaagcgag tcaggacctc tacgcggacg agtggcggac gttccgctac
     3121 gttaccctgc cgacgatacg atctgcggtc ctcggctcgg cgctgctcgt gttcacgctg
     3181 agcctggacg agatcgcggt gacgttcttc ctggtgggcc ggcagaatac gctgccgctc
     3241 cagctctggg gtctgctgcg tcgcggcatc acgccggagg tcaacgctgc ggcgaccgtc
     3301 atcttcgtcg tctcggtcgc ggtcatcgtc ctcgctgcgc gcgcgatgca gcagaatccg
     3361 gattgacagc cctcgggccg cgccgtacat tcgcgcaacc atcctctatg ccaacgccac
     3421 ataggcgctt caccctgcac ggcgcgccgc ttcgcacggt cgcggagttg cagtgcatcg
     3481 cgcgccgttc acatccgccc ggccgctgac gcaccgggag tgaagacgga tcgggagatc
     3541 cggggtcgat agccgaccgg atcgtcgccc agcaggtccc tcactcccac tgagtcggcg
     3601 ctctagcccg tccgctgttt cctaatcagt ccaaggatcg acccgtgagc cctcagcctc
     3661 ttgtgctgtt gctcgctgcc acactcgccg gggtaccgct cggcgaggtg cacgcgcagc
     3721 aaccctttgc cttgaccggc cggatcacca acgagagcgg caaccccgtt ccgaacgcga
     3781 ccgtcgccgt tcccgcgctg gagatcggca cgacggtgcg gtccgacggc agctacaccc
     3841 tggcactccc ggatcgggcc aggggccaga cggtcaccct ctcggtgcgg gcgatcggct
     3901 acaagccgtt cgcggttcag gtcgcggtcg gtgagaccgg cgcgacccag gatttcaagc
     3961 tcgagaa
//
LOCUS       DASRJQ010000393         2007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_435057, whole genome shotgun sequence.
ACCESSION   DASRJQ010000393 DASRJQ010000000
VERSION     DASRJQ010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435057"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VFB89_08845"
     CDS             complement(<1..255)
                     /locus_tag="VFB89_08845"
                     /inference="COORDINATES: protein motif:HMM:NF014769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27451.1"
                     /translation="MTPDFAADADRLAAIALAEDGSRDITSEVTVAGTARGTGRLEFR
                     SGGVVAGQRYAQAVAQLCGCRIEWATAEGEQLPAKAVLGNA"
     gene            complement(252..1247)
                     /gene="nadA"
                     /locus_tag="VFB89_08850"
     CDS             complement(252..1247)
                     /gene="nadA"
                     /locus_tag="VFB89_08850"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HZM27452.1"
                     /translation="MSVPLPVIEEFDLSAEATRALQTEIRERARERNAVILAHNYQRP
                     EVQDIADFVGDSLGLSRQAAKADADVIVFCGVHFMAETAKILSPQKKVLLPDLRAGCS
                     LAATITGAQVREWKAQFPDYVVVGYVNTTAEVKAELDYACTSGNVLDVIDAIPANKGI
                     LFLPDFFLGSHIRRMRPDRKVEVWLGECHVHKGINPNVLEAQRAAHPEAEVLVHPECG
                     CAGQLIYEMGRGAIAANGLHIASTEGMIRLVKERPANSFIIATESGIMHRMKQIAPDK
                     QFYPADPEAVCAFMKSITLPGVRDALALDRYEITVPEDIARRARLSLDRMVSLGK"
     gene            complement(1247..>2007)
                     /locus_tag="VFB89_08855"
     CDS             complement(1247..>2007)
                     /locus_tag="VFB89_08855"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZM27453.1"
                     /translation="AVRGAGAYLVDTTGRRFAFDYDARGELAPRDILARAVAAADANG
                     GAWLDARAVSDFREQFPGISQMLASHGLDPARDLLPVAPALHYAMGGVRTDLDGHSSL
                     PGLWAVGECASTGVHGANRLASNSLLEGLVFADRVARSLPPPAVVDLPTAAAPGERVR
                     DVTPTPASEAIRARMRAIMTADVGVQRSEVSLLRAEEALAELTRELSPDDWRTANQLL
                     VATLITRAARSRRESRGGHRRVDYPPKARQSKVV"
BASE COUNT          328 a          670 c          646 g          363 t
ORIGIN      
        1 ggcgttgccg agcacggctt tggctgggag ctgctcgcct tccgccgtcg cccattcgat
       61 tcggcagccg cacaactgcg ctacggcttg cgcgtatcgt tgccccgcca ccacaccacc
      121 gctccgaaac tcgagccgcc cggttccgcg tgccgtgccg gcgaccgtca cttcactggt
      181 gatatcccgg gatccgtctt ccgccagcgc gatcgccgcc agccggtcgg cgtcggctgc
      241 gaagtcgggc gtcacttgcc caggctcacc atacgatcaa gcgataatcg ggcccggcga
      301 gcgatgtcct ctgggacagt gatctcgtag cgatcgaggg caagcgcatc ccgtacgccc
      361 gggagggtga tgcttttcat gaacgcgcag accgcttcgg gatccgccgg atagaattgc
      421 ttgtcgggcg cgatctgctt catgcggtgc atgatgccgc tctcggtggc gataatgaat
      481 gaattggcgg gacgctcctt cactagcctg atcatcccct cggtcgaggc gatgtggagc
      541 ccattcgccg cgatggcgcc gcggcccatt tcgtagatga gctgtccggc gcagccacac
      601 tcgggatgca ccagcacttc ggcctccggg tgagcggcgc gctgtgcctc gagcacattc
      661 ggattgatgc ccttgtgcac gtggcactcc ccgagccaca cctcgacctt ccggtcgggg
      721 cgcattcgcc gaatgtgcga gccgagaaag aagtcgggca gaaacaggat gcccttgttc
      781 gcagggatcg cgtcgatcac gtccaggaca tttccgctgg tgcaggcgta atccagctcg
      841 gccttcacct cggctgtcgt gttcacgtat cccacgacga cgtagtcagg gaattgtgct
      901 ttccactcgc ggacctgcgc tccagtgatg gtggccgcga gcgagcagcc cgcgcggaga
      961 tccggtaaca ggactttctt ctgcggcgac aggatcttcg ctgtttccgc catgaaatgc
     1021 acaccgcaga acacgatcac gtccgcgtct gccttggctg cctgccgcga caagcccagc
     1081 gagtcaccca cgaagtcggc gatatcctgc acctcgggac gctggtagtt gtgagccaga
     1141 atgacggcgt tgcgctcccg cgcccgctcg cggatctcgg tctggagcgc tctcgtcgct
     1201 tcggcgctga ggtcgaactc ttcgatgacc gggaggggaa ccgacattag accactttcg
     1261 actgtcgagc tttgggaggg tagtccacgc gccggtgtcc accgcgactc tcgcggcggc
     1321 tccgggccgc tcgggtaatg agcgtggcga cgaggagctg gttggcggtt cgccagtcat
     1381 ccggcgacag ctcgcgtgtc agttcggcga gtgcctcttc cgctctgagc aacgatacct
     1441 cgctccgctg gacgccgacg tctgcggtca tgatcgcgcg catcctggcg cggatcgcct
     1501 cacttgccgg cgtcggggtg acgtcccgta ctcgttcacc gggagcagcg gcggtcggca
     1561 agtctaccac cgccggcggc gggagcgacc gcgcgactcg atccgcgaac accagccctt
     1621 cgaggagcga gttggaagca agccgattgg caccgtgaac tccggtgctg gcacattccc
     1681 cgacggccca cagtccggga aggctggaat gaccgtcgag atcggttcgt actccgccca
     1741 ttgcgtagtg cagggccgga gcgaccggaa gcagatcgcg agccgggtcg agtccgtggc
     1801 tggcgagcat ctgcgaaatc cccggaaact gctccctgaa atcgctgacc gcacgggcat
     1861 cgagccaggc cccgccattc gcgtccgcgg cggcgacggc gcgagcgaga atgtcgcgcg
     1921 gcgcgagctc tccgcgggca tcgtagtcga acgcaaaccg gcgacccgtc gtgtccacca
     1981 ggtaggcgcc cgccccacgg acagctt
//
LOCUS       DASRJQ010000394         4337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3576572, whole genome shotgun sequence.
ACCESSION   DASRJQ010000394 DASRJQ010000000
VERSION     DASRJQ010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3576572"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VFB89_08860"
     CDS             complement(<1..828)
                     /locus_tag="VFB89_08860"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZM27454.1"
                     /translation="MNPEEVAAEVRRIEITTRHLVRDIVAGEYSSAFRGRGVEFAEVR
                     EYQPGDDVRTIDWNVTARLGTAYVKRFLEERELTVLFVVDYSASDSFGSGQQSKRSLA
                     TEVCAVIALAAARSNDRVGAAFFTDRLERYVPPRKGRRQVLRVVSELLSYQPVGQGTD
                     LAGSLLVLEPLLRRRSVLFVVSDFIADNWGPILGRLSRRHDVIAVQLVDPRERELPDV
                     GVVALRDAETDEWCYVDTGSPSVQAHLRARVAAFDAQVDRTVRENRADLLRLDAGAPY
                     "
     gene            complement(825..1847)
                     /locus_tag="VFB89_08865"
     CDS             complement(825..1847)
                     /locus_tag="VFB89_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZM27455.1"
                     /translation="MSMLPDPAAAPVRTSTTTPDHAHAEESRLAAEALTELGRVVVGQ
                     RSLLERLLIGLLADGHVLLEGVPGLAKTLAVRTLAAIVSGGFRRIQFTPDLLPADLIG
                     TQVYNQRSGEFTVQKGPIFTNILLADEINRAPAKVQSALLEAMQEKQVTIGGQSLALP
                     DLFLVLATQNPIEHEGTYPLPEAQVDRFMFKLVVTYPQRDEERQVLDRMTAGPEPTAS
                     PVLDAARVLEARRLVQDLYVDDRVKEYVLDVIGATRDPGAVGLKELRPLVEYGASPRA
                     GIFLLRAAKAHAFLAGRGYVTPEDVKALALDTLRHRVILTYRADADGVTPDILLERLL
                     AAVPVP"
     gene            1941..2771
                     /locus_tag="VFB89_08870"
     CDS             1941..2771
                     /locus_tag="VFB89_08870"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZM27456.1"
                     /translation="MTGVIQDGQFYGKVAGSRRLEDFVLAETRYPGRAVVPWHAHESP
                     LVCFVIRGALEERTEAGSRQYRSGAVFYHPMLEPHSHRFEPAGAQCFTLTMRAEMFRR
                     LEPRDRVELHAPLDASTSPIAWLVRRLHAEFQHGAAASELVLEGLALAVLGELTRWPG
                     PGSGNGRPVWVETVRDLAETHCDKSVSLTALAAEVGIHPVHLARSFRRAFGCSLGEYA
                     RRARIERARQALLATDQSLASIALMAGFSDQAHFCRQFKLATGTTPGAYRAAGRQTRD
                     "
     gene            complement(2756..3445)
                     /locus_tag="VFB89_08875"
     CDS             complement(2756..3445)
                     /locus_tag="VFB89_08875"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HZM27457.1"
                     /translation="MQYIARSISHLPGMQRRSFLFGSAAVAASFAARRVAGLTSATTP
                     IAFLIGEHVNVIDMAGPWEVFQDVMLPNGGESAFELFTVGPSKAPLAATGGLTIVPHY
                     SYSDAPRAAIVVVPAHHSSAATLEWLRAVARRADLVMSVCTGAFIVARAGLLDGLTAT
                     THHEFQDSFAREFPKVKLERGVRYVEHEKVATAGGLTSGIDLALRVVERYHGAEIARA
                     TATYLEYQSRV"
     gene            complement(3500..>4337)
                     /locus_tag="VFB89_08880"
     CDS             complement(3500..>4337)
                     /locus_tag="VFB89_08880"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HZM27458.1"
                     /translation="RTPVTNQEFERFVAASGYVTVAERRLDPKDFPDLPKERLAPGSI
                     VFSPPKHDLTYLSSLEWWRWVPGANWRHPEGPGSDLRGRSNHPVVQVTYEDAEAYARW
                     VGKRLPTEAEYEWAARGGLDRKRYAWGNELKPGGRYVANIFEGRFPTRNTGEDGYVGT
                     SPVTAFPPNGFGLYDMGGNVWQWTSDWYRTDWYARLAAAGIARNPTGPPTSLDPSHPD
                     SPTRVIRGGSYLCSEQYCTRYLVGSRGRGEASTGSSNLGFRLVRSVGDSTARPGQVRG
                     TQ"
BASE COUNT          742 a         1409 c         1427 g          759 t
ORIGIN      
        1 atagggtgcc ccggcatcga gccgcagcag gtcagcccgg ttctcccgaa ccgtacgatc
       61 gacctgtgca tcgaacgccg caacgcgggc gcggagatga gcctgtaccg acgggctgcc
      121 ggtgtccacg tagcaccact catccgtttc cgcatctcgc aaggccacca ctcccacgtc
      181 cggcagctcc cgttcgcgcg gatcgaccag ttgcaccgca atgacgtcgt gccgccggga
      241 caaccggccc aggatcggtc cccagttgtc cgcgatgaag tccgagacga cgaacagaac
      301 cgagcggcgc cgcagcaatg gctcgaggac gagcaggctg ccggccaggt ctgttccctg
      361 acccacgggc tgataactca gcagctcgct caccacgcgt aacacctgcc gccgtccttt
      421 tcgtggcggg acgtagcgct ccagccggtc ggtgaagaag gccgcgccca cccgatcgtt
      481 gctccgcgcg gctgcgagcg ctatgacggc gcagacctcg gtggccagcg agcgcttgct
      541 ttgctgccct gagccgaagg agtccgacgc gctgtagtcg accacgaaca ggaccgtcag
      601 ctcgcgctct tccaggaaac gcttgacgta tgcggtcccc agtctcgccg tgacgttcca
      661 gtcgatcgtg cgtacatcgt cgcccggttg gtattcgcgg acctcggcga attcgacgcc
      721 ccggcctcgg aaggcgctgg agtactctcc cgcgacgata tcccggacca ggtgtcgtgt
      781 cgtgatctcg atccggcgga cttcggcggc aacttcctcg ggattcacgg gaccggtacg
      841 gcggcgagca gtcgttcgag taggatgtcg ggagtgacgc catcggcgtc ggcgcgatag
      901 gtgagaatga cccggtggcg cagcgtgtct agcgccagag cctttacgtc ttccggggtg
      961 acatatccgc gaccggcgag gaacgcgtgc gccttggcgg cgcggagcag aaaaatgccg
     1021 gcacggggtg aggcgccgta ctccaccaat ggtcgcagtt ccttgagccc gacggcgccg
     1081 gggtctcggg tcgccccgat gacgtcgagc acgtattcct tcacccggtc gtcgacgtag
     1141 agatcctgaa ccagacgacg ggcctcgagc accctggcgg catcgaggac cggcgatgcc
     1201 gtcggctccg ggccggcggt cattcggtcg agcacctgtc gttcttcatc ccgctgggga
     1261 taggtgacga ccagcttgaa catgaagcga tccacttgcg cttcgggcag cggataggtg
     1321 ccttcgtgct cgatgggatt ctgagtggcg agcacgagga acaaatcggg aagtgcgaga
     1381 ctctggccgc cgatggttac ctgcttctcc tgcattgcct cgagcagcgc gctctgcact
     1441 ttggccggtg cccgattgat ctcgtcggcc agcaggatgt tggtgaagat gggccctttc
     1501 tgaacggtga actccccgct tcgctggttg tagacctggg tgccgatcag gtcggccggg
     1561 agaagatccg gggtgaactg aattcgccga aatcctccgg acacgatcgc agccagggtt
     1621 cgcaccgcca gcgttttggc gagtcccggc accccctcga gcaggacatg cccatccgcc
     1681 agaagtccga tcaacagccg ctcgagcaga cttcgctggc caaccacgac ccgtccaagc
     1741 tcggtcaggg cctcggcagc gaggcgactc tcctcggcat gggcatgatc cggtgtcgtg
     1801 gtcgacgtcc ggaccggggc cgctgcaggg tcgggcagca tgctcatgcg cgaagcctaa
     1861 ccgcaacacg tcacctcagc gaggcccgtc caggtgggcc tcgaatccag ctgaccgccg
     1921 ggttcgtagg gcgtaggcac atgaccggcg tcattcagga cggacaattc tacgggaagg
     1981 tggcgggaag ccgccggttg gaggacttcg ttttggccga aacgaggtac cccggtcggg
     2041 cggtggtgcc ctggcatgcg catgaaagcc cgttggtgtg cttcgtgatt cgcggagcgc
     2101 tcgaagagcg caccgaagca ggttctcggc agtatcggag cggagcggtg ttctaccacc
     2161 ccatgctcga gccgcattcc catcgattcg aacccgccgg ggctcagtgc ttcaccttga
     2221 cgatgcgagc ggaaatgttc cgtcgcctcg agccgcgcga tcgggtcgag cttcacgcac
     2281 cgctcgatgc cagcacgagc ccgatcgcct ggctggtgcg ccgactgcat gccgagttcc
     2341 agcacggtgc cgccgcttcg gagctggtgc tggaaggcct cgcgctcgct gtgctcggcg
     2401 agctgacgcg ctggcccggc cccgggtcag ggaatgggcg gccggtctgg gtggagacgg
     2461 ttcgcgatct tgcggaaacg cattgcgaca agtcagtgag cctcaccgct ctcgcggcgg
     2521 aggtcgggat tcatcccgtg catctggccc gctcgttccg gcgcgccttc gggtgcagcc
     2581 tgggcgaata cgcgcgccgc gcacggatcg agcgggcgcg ccaagcgctg ctcgcgaccg
     2641 atcaatcgct ggcgagcatt gcgttaatgg ccggattttc cgatcaggct cacttctgcc
     2701 gtcagttcaa gctcgccacc gggacaaccc ccggcgccta tcgggccgcg gggcgtcaga
     2761 ctcgggactg atactcgagg taagtcgcgg tggcgcgggc aatctcggcg ccgtggtacc
     2821 gctcaacgac ccggagagcg agatcgattc ccgaggtcag acctccggca gtggcgacct
     2881 tctcgtgctc tacatatcgc acgccccgct ccagcttcac cttgggaaac tcgcgggcga
     2941 acgagtcctg gaattcgtgg tgcgtagttg cggtgagacc atccagtaat ccagcccgcg
     3001 ccacaatgaa cgcgccggtg cacaccgaca tcaccagatc ggcgcgtcgc gcgactgccc
     3061 ggagccactc gagtgtcgct gccgagctgt gatgagcggg aactaccaca atggcggctc
     3121 tcggcgcgtc cgagtagctg tagtgtggca cgatggtaag cccaccggtt gcagcgaggg
     3181 gcgccttact cggcccgacc gtaaacagtt cgaacgcaga ctctccgccg ttcggtagca
     3241 tgacgtcctg gaagacctcc caggggcccg ccatgtcgat cacgttgacg tgctctccga
     3301 tcaggaacgc gatcggcgta gttgcgctgg tgaggccggc gactcgccgg gccgcgaagc
     3361 tcgccgcgac ggccgcggag ccgaagagaa acgatctgcg ttgcatgcca ggaaggtggg
     3421 aaatcgagcg ggcgatgtat tgcaccggcc gagccgggtg gaacgcagtt agcctgaggc
     3481 tctcaagcag tgtcgagcgt cattgagtcc cgcgaacctg cccgggacgc gcggtcgaat
     3541 cgccgactga ccgcaccaga cggaatccca gattggaaga gccggtgctc gcctcgccgc
     3601 ggccgcgact gccgacgaga tagcgggtgc agtactgctc gctgcacaga taggagcctc
     3661 cccggatgac ccgggtcggc gagtcggggt gcgacgggtc gaggctggtt ggcgggccgg
     3721 tcggattccg ggctatcccc gctgcggcca atcgagcgta ccagtcggtg cgataccagt
     3781 cgctcgtcca ctgccacacg ttgccaccca tgtcgtacag tccgaagcca ttgggcggaa
     3841 acgccgtcac cggcgatgtg cctacgtagc catcttcccc ggtattgcgg gttggaaacc
     3901 gtccttcgaa aatgttcgcc acatagcggc cacccggctt gagctcgttc ccccaggcgt
     3961 aacgcttccg gtcgagcccc ccacgcgcgg cccattcata ttcggcctcg gtaggcagcc
     4021 tcttgccgac ccagcgcgcg taggcctcgg cgtcctcata agtcacttga actaccgggt
     4081 gattggagcg accgcggagg tcgctgccgg gtccttcggg gtgacgccag tttgctcctg
     4141 gtacccagcg ccaccattcg aggctgctca gatacgtcag atcgtgcttg ggaggtgaaa
     4201 acacgatcga ccccggagcg agccgctctt tcggcaggtc gggaaagtcc ttcggatcga
     4261 gccggcgctc cgcgacggtc acgtatccgc ttgctgcaac gaaacgctcg aactcctgat
     4321 tggtgaccgg tgtgcga
//
LOCUS       DASRJQ010000395         4259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2027379, whole genome shotgun sequence.
ACCESSION   DASRJQ010000395 DASRJQ010000000
VERSION     DASRJQ010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2027379"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..1554)
                     /locus_tag="VFB89_08885"
     CDS             complement(4..1554)
                     /locus_tag="VFB89_08885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HZM27459.1"
                     /translation="MLARVRSAAVLGVDAYPVDVEVDLSNGLPSLATVGLPQSAVKEG
                     RERVHAAIANAGFEFPLKRITVNLAPADRPKAGSGFDLAIALGILVASGQVPAERLVD
                     LLVLAEIGLEGDLRPIRGALPVALCAKAMGVPGLLLPAANAPEGALVEGLAVRGATSL
                     AEVCAFLSGRGELPPVRSNGAHVLQTGGDGAADFSEVRSQATAKRALEIAAAGGHNVL
                     MIGPPGAGKTMLARRLPSILPTMSLEEALEATKIHSVAGTLPPGKALIEARPFRAPHH
                     SVSDAGMIGGGSWPRPGEVSLSHGGVLFLDEVPEFRRNVLESLRQPLEDGVVTLSRAA
                     MTLSFPARFMLVGAMNPCPCGHLGDPVRTCRCEPILVDRYRARISGPLLDRIDIHLTV
                     PPVPVGDLSAGGPAVESSAAIRARVESARRIQQERFAGQAGVYGNAHMSPRDVRRHCK
                     LAHTADALLREAISRLGLSARAYTRVLKLSRTIADLAGHAEITAAHVSEAIQYRVLDR
                     LRLPAHAG"
     gene            1794..3158
                     /locus_tag="VFB89_08890"
     CDS             1794..3158
                     /locus_tag="VFB89_08890"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HZM27460.1"
                     /translation="MKRLAMLGLVAVISFLTGGWFLHREPTEEERLYEQARVLGTVLS
                     VIHEHSLESADQNELFQNTARNLVAQLKDPYAELLVGDGYRQFSRQMSGTGELDTPAA
                     SATEPKRVHIPATSPGVMLNTTTGYVALHSLSEGSSDELQSAVWGLRKRGMRTLVLDL
                     RNDPGGLIKQGVQVAELFLPKGDTIVITKGRTPEHSRVYVAASGEKWPNMGLVVLVND
                     GTASSAELIAGALQDHDRAAIIGLPTYGKGVLQTTYPLGSDMAVKLTTARWYTPSGRS
                     INRPRTRADSVLADARQSALTHGTRYRTDSGRLLADGKGIQPDRIIRRDSFTPDERAF
                     LDSLGSGYAAFRGALSDCATAIKQQGEITSEDFVVTPAMRERVRALLEDRQLHIGDKL
                     FAGGAAYVDRQLGYDIAREVFGDAAASRRRLLDDRQMQTALEMIQHSASEGDLIRLAE
                     STAD"
     gene            complement(3155..>4259)
                     /locus_tag="VFB89_08895"
     CDS             complement(3155..>4259)
                     /locus_tag="VFB89_08895"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZM27461.1"
                     /translation="PPSEFEPVFGAFERMAADIRSSQSALEEARRRTAAVLATVATGV
                     VGLDQGGHVIIANRQAVDLLGLPLAEGDLLLDYLGDEWTALAEAVRRFLLSGGEAESS
                     AELTVGGRRLTLQLASLGPDLRGAVLALNDVTNLSRAERVLAWGEMARQVAHEIKNPL
                     TPMRLGVQHLRRVWRDRRDEFGGALEETAGRILAEIDRLDTIARAFSRFAAPALAQDP
                     LEPVDLAAVASEVVHLYQLAGEAAGATVRLDVIEHAWGDSRRDELKEVLVNLLENARN
                     AGARSIVVTVSVTCIQVRDDGSGIPVHLLPRIFEPRFSTTTSGSGLGLAIVRRLVESW
                     GGSVEVDSEVGQGTSATIRFATGVAPTAAAGGN"
BASE COUNT          754 a         1455 c         1366 g          684 t
ORIGIN      
        1 attctaccca gcatgggccg gtaaccgcag ccggtccagc acgcgatact gaattgcctc
       61 gctcacatgg gccgccgtga tttccgcgtg gcccgccagg tcggcaatgg tgcgcgacag
      121 cttgagcacg cgggtgtagg cccgggctga gaggcccagc ctcgaaatgg cctctcgtag
      181 aagtgcgtcc gcagtgtgag ccagcttgca gtgccgccgc acgtcacgcg gcgacatgtg
      241 ggcgttcccg tacacgcccg cctggccggc gaagcgttcc tgctgaatcc gccgggctga
      301 ttcgacgcgc gctcgtatgg cggcgctcga ttcgacggcc ggaccgccgg ccgacaagtc
      361 gcctacagga accggcggca cagtcaaatg gatgtcgatg cgatcgagca gcggtcccga
      421 aatacgtgcc cggtagcgat ccaccaggat tggctcgcac cggcacgttc ggaccggatc
      481 gccgagatgg ccgcacgggc aaggattcat cgcgccgacc agcatgaagc gcgcgggaaa
      541 cgacaaggtc atcgccgccc ggctcagcgt taccacgccg tcctcgagag gctgccgtaa
      601 cgattcgagc acgttgcgcc tgaattcggg aacctcgtcg aggaagagca cgcccccatg
      661 gcttagactc acttcgccgg gcctgggcca gctgccgcca ccgatcatgc cggcatcact
      721 cacggaatga tgcggcgccc ggaacggccg ggcttcgatc aacgctttac ccggaggcaa
      781 cgttccggcc acactgtgga tcttcgtggc ctccagcgcc tcttccaggc tcatcgttgg
      841 caggatggag ggcagtcggc gggcgagcat tgtcttgccg gcccctggag ggccaatcat
      901 gagcacgttg tggccgccgg ccgccgcaat ttcgagcgca cgcttggccg tcgcctggct
      961 gcgcacttct gagaagtccg ccgcaccatc gccgcctgtc tggagtacat gcgcgccatt
     1021 ggagcgcacc ggtggaagct cccctcgccc gctcaggaag gcgcagacct ccgccaggga
     1081 ggtcgcgcca cgtaccgcga gtccctcgac gagggctcct tctggtgcgt tggccgcagg
     1141 aaggagcagt cccggcactc ccattgcctt ggcacagagt gcgacgggca acgcaccgcg
     1201 gatgggccgc aggtctccct cgaggccgat ttcagccagc accaggagat cgaccagccg
     1261 ctcggccggt acctgcccgc tcgccaccag gattcccagt gcaatcgcga ggtcgaatcc
     1321 cgagccggcc tttggccggt cggccggagc caggttcact gtgattcgct tgaggggaaa
     1381 ctcgaagccg gcgttcgcaa tcgcggcgtg aactcgctcc cggccttcct tcaccgcgct
     1441 ctgcggcaac cccaccgtcg ccagcgacgg caggccgttt gaaaggtcaa cctcgacatc
     1501 gacgggatag gcgtcgacgc cgagaacggc tgcggagcga acacgtgcga gcatcggtac
     1561 ccggcgcggc gatcgtccga gagtagccga tcagcgacga cgggccgcat tccgcgcacg
     1621 catttcggtg ctccaacgcg cgatgatcgg ggggcgccga gcggtggcaa acacggcccc
     1681 tgatttgcac tacggcggat ggacaaccac gcaagcggcg ccgtgacgct gtgttagggg
     1741 cgcccatcgt ctgcaaccgg caggactagc tggcaattcg cggcaaggct tccatgaaac
     1801 gcttagccat gctgggtctc gtcgcagtca tctccttcct cacgggcggc tggttccttc
     1861 accgcgagcc caccgaggag gaacgattgt acgagcaggc tcgcgtgctc ggaacagtcc
     1921 tctcggtcat ccacgagcac tcgctcgaat ccgccgatca gaacgaactg tttcaaaaca
     1981 ctgctcgaaa tcttgtcgcc caactgaagg acccgtacgc cgagcttctg gtcggagacg
     2041 gctaccggca gttcagccgg cagatgtcag gcaccggcga gctggacacc cccgctgcgt
     2101 cggcgaccga gcccaaacgg gtgcacatcc cggccacctc tcctggggtc atgctcaaca
     2161 ccaccacggg atacgttgcc ctccattcgc tgagcgaagg ctcgtccgat gagctacaga
     2221 gcgccgtgtg ggggctgcgg aagcgcggaa tgcggaccct ggtgctcgat cttcgcaacg
     2281 accccggagg cctgatcaag cagggtgttc aggtggccga gctgttcctg cccaagggcg
     2341 acaccatagt gatcacgaag ggccgcaccc cggagcactc gcgggtgtac gtggcggcgt
     2401 caggcgagaa gtggcccaac atgggattag tggtcctggt aaacgatggc actgccagct
     2461 ccgccgagct gatcgcggga gcactgcagg accacgaccg ggccgcgatc atcggactgc
     2521 ccacgtacgg gaagggcgtg ctgcaaacga cctacccgct gggcagcgac atggccgtca
     2581 agctcaccac ggcgcgctgg tacaccccca gtggtcgaag catcaaccgg ccgcggaccc
     2641 gggcggacag cgttctggcc gatgctcggc agtccgctct cacgcatggc actcgctacc
     2701 gcaccgacag cggccgcctg ctggctgatg gcaagggcat ccagcccgat cgcatcatcc
     2761 ggcgcgacag cttcacccct gatgaacggg cattcctgga ttcgctgggc agcggttacg
     2821 ccgccttccg tggagctctg tccgactgtg ccactgcgat caagcagcag ggcgagatta
     2881 cgtccgaaga cttcgtggta acgccagcga tgcgcgagcg ggtgcgcgcg ttgctggagg
     2941 atcggcaact gcacattggc gacaagctgt ttgccggtgg ggcggcctac gtcgaccggc
     3001 agctgggcta cgacatcgcc cgcgaggtgt tcggggatgc cgcggcgagc cggcgccggc
     3061 tcctcgacga ccgtcagatg cagacagccc tggagatgat ccagcactcg gcttccgagg
     3121 gtgatctgat ccgtctggcc gagtctaccg ccgactagtt tccccctgcc gcggccgtag
     3181 gcgcgactcc ggtcgcgaat cgtatcgtcg ccgaagttcc ctgccccacc tcgctgtcga
     3241 cctcgaccga gccaccccag ctttcaacca atcgccgcac aatcgccagg cccaggcccg
     3301 acccgctggt cgtggtcgag aatcttggct cgaaaatccg gggaagcagg tgcacgggaa
     3361 tgcccgatcc atcgtcccgc acctggatgc aggtaacgct cacggtgacg acgatcgagc
     3421 gcgccccggc attacgggcg ttctccagga gattgaccag gacttccttg agctcgtccc
     3481 gcctcgagtc gccccaggcg tgctcgatca catcgagtcg aaccgtcgcg ccggccgctt
     3541 ctcccgccag ttgatacaga tgcacgacct cggacgcgac cgcggcgagg tccacaggtt
     3601 caagcgggtc ctgagccagt gccggcgctg caaagcggct gaaggctcgg gcgatggtgt
     3661 cgagccgatc gatctccgcc agaatcctcc cagcggtctc ctccagggcg ccgccaaatt
     3721 catctcgacg gtcacgccag acgcgccgaa ggtgttgcac gccgagcctc atcggagtga
     3781 gcggattctt gatctcatgc gccacctggc gggccatctc accccaggcg agaacccgct
     3841 cggcgcggga tagattggtc acgtcgttga gggcgagcac tgcgccccgg aggtcggggc
     3901 ccagtgatgc gagctggaga gtcagccggc ggccgcccac cgtcaattca gcgctggatt
     3961 cggcttctcc ccccgagagc aggaaccgcc gcaccgcttc tgccaaggcg gtccactcgt
     4021 cgcccaggta gtccagcaaa agatcgccct ccgcgagggg aaggccgagc aggtcgactg
     4081 cctgccggtt cgcgattatc acgtggccgc cctgatcgag accaacgacc ccggtcgcca
     4141 cggttgcgag cacggcggcc gtgcggcgcc gtgcctcttc cagggcgctc tggctcgaac
     4201 gaatgtcggc agccattcgc tcgaacgcgc cgaacaccgg ctcgaactcg ctcggcggg
//
LOCUS       DASRJQ010000396         5009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_4110142, whole genome shotgun sequence.
ACCESSION   DASRJQ010000396 DASRJQ010000000
VERSION     DASRJQ010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4110142"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VFB89_08900"
     CDS             complement(<1..921)
                     /locus_tag="VFB89_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27462.1"
                     /translation="MPTPLASLDSPPRTLTHSPLWFDAPRLVADALVAVREPLTTVLR
                     PEPPAAAAAIACALARSLAPPEARDSAPTWLWPDQVPSFRRALYALRRFGGALLADPV
                     GSGKTYIGLAVASVANGRRSTVCLVPATLVTQWESVARRLNLTVLVWSHERVSRGNLP
                     SAIHGKVVLIDESHHFRNPATRRYGHLAPWLMGRQALLMSASPVVNRLPDLAHQLALT
                     IRDDALAGHGIPSLAGLLEQSRGHSALSNVIVAGPNAEARRPGRSERAVSLDDSSLSP
                     LRDVLVGLDELKLSTQPTVAGLVRSAFWRAA"
     gene            complement(833..3865)
                     /locus_tag="VFB89_08905"
     CDS             complement(833..3865)
                     /locus_tag="VFB89_08905"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HZM27463.1"
                     /translation="MVTAGTLGSLYRLEDLPDFIHALGHQPAWEELPIPGWLGLTTSE
                     LTRAALVGRIGDFQWFGIDAPDAARGAHLVARRLVARGRPAGVVALSRQSRSLFITIA
                     FSDPVCVECTLDQPDHLTLTRLARLGSSPARTAIEFAARAADVLASEAVGRRFFRQFE
                     LVLGRMRDAMPRSCPPQERHGWVLLQLTRVLFLYFVQSKGWLDGTSNFLAREVDRCLG
                     RGRRLQRDLLRPLFFGTLNRPPRERTRRTRGLGRIPFLNGGLFEPHILEQRWRVDLPN
                     AVWRDAFDTLFERFDFTADETGRPGMVAPDMLGRVFEGVMESGIRRESGTFYTPATLV
                     QSLVSATLIALISERLGCGEGRAERYLADRSAPARRALHDITVLDPAVGSGAFLLGAL
                     DILSALVTKRATSTRARRRVLRRCLFGVDLNPTAVRLTELRLWLAVIANDPATDAESV
                     EPLPNLDCLVRQGDSLFDPAGYWPGGIGISRTDARSLARLRQQLVAAVGSGKRALARE
                     LRRAETGAFKAALEAAESALDATLADRLADGRAPTLFGGRRGLDAELRKELCRLRAAR
                     RDVRATRRRFARENEIPWFHFQSQFADVFAKGGFDIVIGNPPWVRAEALPPERRVLLA
                     SRYSWWRSSGRGFSHRPDLSLAFLARAWELARHGGVVGFLVPAKLATAEYGARARHAL
                     AAHGTIHAAADLTRGCEPIFDATVYPLALVVSKTPPPDGHRVRTRLAPRQGSTATGVP
                     QQELTGGKAWPLATAPVLEALGALTRHPRLGDQFSCHLGVKTGANDLFLDPPDSVEPE
                     LIRWAVRGRDVEPFKAIRGRRLLWPCDDSGRPLRELPPGARAFLASHTGRLRQRADDA
                     GGPAWSLFRTAAAAARYRVVWPDLSRELAAVALAEDSERDQIPLNTCYVIVTASLPTA
                     LRLAAWLNATWTRAAARLVAPPAASGFVRFTAQVVAGLPLPDCVLADAALSDFAIAGA
                     RGESIQRDLDAYTARLLGLTSADAHSLAAVVRRAATRR"
     gene            3992..4645
                     /locus_tag="VFB89_08910"
     CDS             3992..4645
                     /locus_tag="VFB89_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27464.1"
                     /translation="MRRSLLAFFLLVPGLAHAQAADSSRFVVLHGADTVATEQLKRTE
                     TELHGTLAFRGARTTELYRAVLAPDQTVPLVEVTVQEAPASSADKARVAQQARIIFRN
                     DSVAIDDISNHGLETRVLPTQSGAIPYINLSFGLLEQAIRRAAVLGKDSVKVAFFNLS
                     GSASQGGGATVVGTVARFAVDSVSLDLGQVEFRLRVDSQGRLLGGGIPTQRLSFTRH"
     gene            complement(4647..>5009)
                     /locus_tag="VFB89_08915"
     CDS             complement(4647..>5009)
                     /locus_tag="VFB89_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27465.1"
                     /translation="GWSKNIYIGGRKTFPDEPIRRALVPMMLSGTMLFWLLPPILLLL
                     AFFGVVPWMGWALVAVALSALFWMLVSVGMGIPVLHGLAYPAGALVGLYIILRSVIRG
                     ARRIEWKGRVYDIRPGLG"
BASE COUNT          921 a         1636 c         1675 g          777 t
ORIGIN      
        1 ggccgctcgc cagaacgcgg agcgaacaag acctgcgacc gtaggctggg tagagagctt
       61 cagttcgtcc aggccgacca gtacgtcccg cagcggcgac aggctcgagt cgtcgagcga
      121 cacggcccgc tcactccttc cggggcgccg cgcctccgcg ttcgggcccg ccacgatgac
      181 gttgctcagc gcggaatgcc cgcggctctg ctccagcagt cccgccagcg atggaatccc
      241 gtgaccagcc agtgcatcgt cccggattgt gagtgccagt tggtgtgcga gatctggcaa
      301 gcgatttact accggcgatg cgctcatcaa cagtgcctgg cggcccatca gccatggggc
      361 caagtggccg tatcgccgcg tagccggatt gcggaaatgg tggctttcgt cgatcagaac
      421 gaccttgcca tggattgccg atggcaggtt cccacggctc accctttcgt gggaccacac
      481 tagaacggtc agattgagcc gcctcgcgac cgactcccac tgagtcacca gcgtcgccgg
      541 aaccagacaa acggtggagc gcctgccatt cgccaccgat gccacggcca gtccgatata
      601 cgtcttccca ctgccgacgg ggtctgccag caaggctccg ccgaagcgtc ggagcgcgta
      661 cagcgcgcgg cgaaacgaag ggacctggtc gggccagagc caggtgggcg cggaatcacg
      721 agcctcgggt ggtgcgaggc ttcgcgcgag cgcacacgcg attgccgccg cggccgctgg
      781 tggctccggc cgcaagaccg tcgtgagcgg ctctcgcacc gccacgagcg cgtcagcgac
      841 gagtcgcggc gcgtcgaacc acagcggcga gtgagtgagc gtccgcggag gtgagtccaa
      901 ggaggcgagc ggtgtaggca tcgaggtccc gctggatgga ctccccgcgc gcaccggcga
      961 tcgcaaagtc gctgagcgct gcatcagcga gcacgcagtc cggcaggggg aggccggcga
     1021 ccacctgggc cgtgaaacgg acaaacccgc tcgcagccgg cggcgccacc agccgtgctg
     1081 ccgcacgagt ccacgtcgcg ttgagccagg cggccaggcg aagtgcagtc ggcagacttg
     1141 cggtgacaat gacgtaacag gtgttgagcg ggatctggtc tcgctcgctg tcctcagcca
     1201 gcgcaacggc ggcgagctcg cgactcaggt cgggccagac cactcggtat cgagccgcgg
     1261 cggccgcggt tcgaaagagg ctccacgctg gtcctcccgc gtcatccgcg cgttgtcgaa
     1321 gtcggccagt gtgcgaggca aggaacgccc tggctcccgg cggaagctcc ctgagagggc
     1381 ggcccgagtc gtcacagggc cagagcaagc ggcgcccgcg gatagccttg aacggttcga
     1441 catcgcggcc gcgcaccgcc cagcggatca gttccggctc gacgctgtcc ggcggatcaa
     1501 gaaagaggtc gttggctcct gttttcactc cgaggtggca ggagaactga tcgcccaggc
     1561 gcgggtgtcg agtcagggcg cccagcgcct cgagcacggg cgccgtggcc agcggccacg
     1621 ctttgccgcc ggtgagttct tgctgcggaa ctcccgttgc cgtcgaaccc tgccgggggg
     1681 cgagccgggt gcgtacccga tgtccgtccg gtggcggtgt cttgctcacc accagtgcga
     1741 gcggatacac cgtcgcatca aagatcggct cgcagcctcg ggtgaggtcg gccgccgcgt
     1801 ggattgtgcc gtgtgccgcg agcgcgtgtc gggcacgagc accgtattcg gcggtcgcga
     1861 gcttggccgg aaccaggaac ccgacgacac cgccatggcg cgccagctcc caggcccgcg
     1921 cgagaaacgc gagcgagagg tcaggccgat gagaaaaccc acggccgctg ctccgccacc
     1981 acgagtaacg cgacgcgagc agcacccgac gctccggcgg aagcgcttca gctcgaaccc
     2041 aaggtggatt gcctatgacg atatcgaagc cacccttggc gaaaacgtcg gcgaactgac
     2101 tttggaagtg gaaccagggg atctcgttct ctcgcgcgaa gcggcgacga gtggcccgca
     2161 cgtctcggcg cgcggcgcga agccggcaca actccttccg cagctccgcg tccaggccgc
     2221 ggcgaccccc gaacagggtc ggggctcgcc catcggcgag tcgatcagcg agcgtggcgt
     2281 cgagcgctga ctcagcggcc tcgagtgctg ccttgaaggc ccccgtctcg gcgcgccgca
     2341 gttctcgcgc gagggcacgc ttgccggacc ccaccgcggc cacgagctgt tgtcggaggc
     2401 gtgcaagcga acgagcgtcg gtgcgcgaaa tgccaatgcc tcccggccag tacccagccg
     2461 ggtcgaagag actgtccccc tgacggacca ggcaatccag attggggagg ggctcgacgg
     2521 attcggcatc agtcgccggg tcattggcga tgacagcgag ccagagacgc agctcggtca
     2581 gtcgcacggc ggtgggattc aggtcgacgc cgaagagaca tcggcgaagc acccggcggc
     2641 gggcccgtgt cgaagtcgcg cgcttggtga ccagggcaga aagaatatcg agtgcgccga
     2701 gcagaaaggc gccggagccg actgccggat ccagcactgt tatgtcgtga agcgcccgac
     2761 gagccggggc gctgcgatcg gcgaggtaac gttcggcccg cccctcacca cagccgagcc
     2821 gctcgctgat cagcgcaatc agcgttgcgc tgacaaggct ttgcaccagt gtcgccgggg
     2881 tgtagaacgt gccgctctcc cgccgtattc ccgactccat aactccctcg aacactcgcc
     2941 ccagcatgtc cggtgcgacc atgccgggtc tgccggtttc gtcggcggta aagtcgaagc
     3001 gctcgaatag ggtatcgaac gcgtcccgcc acacggcgtt cgggagatcg actctccagc
     3061 gttgttccag gatgtgtggc tcgaacagac cgccgttgag aaacggaatg cgacccaatc
     3121 cgcgagtgcg gcgagttcgc tccctcggcg gccgattcaa cgtgccgaaa aagagcggtc
     3181 gcagcagatc acgttggagg cgcctgccgc gcccgagaca gcgatcgact tctcgcgcca
     3241 ggaagttcga cgtgccgtcg agccatccct tgctctggac gaagtagagg aagagaactc
     3301 tggtgagctg aagcagcacc cagccgtgtc gttcctgcgg tgggcaggag cggggcatgg
     3361 cgtctcgcat gcggcccagg accagctcga actggcggaa gaaccgccgt ccaacggctt
     3421 ccgaagcgag cacgtcggca gctcgcgcgg cgaactcgat tgccgtcctg gctggagacg
     3481 aacccaggcg agcgagccgg gtgagcgtga gatggtccgg ctgatcgagc gtacactcga
     3541 cgcacacagg gtccgagaac gcgatggtaa tgaagagaga gcgagactgc cggctgagcg
     3601 cgactacgcc cgcgggacgc ccccgcgcga ccagtcgccg agccactagg tgagccccgc
     3661 gcgctgcgtc cggagcatcg atcccaaacc attggaagtc tccgattcgc cctaccaatg
     3721 cagcgcgggt cagctcactc gtagtgagcc cgagccagcc tggaatcggc agctcctccc
     3781 acgcgggttg atgccccaag gcgtggatga agtcgggcag gtcttcgagg cggtacagcg
     3841 agccgagcgt gccggcagtg accacgcgtc cgaggctccg tgtcggggag cctcacgttt
     3901 gtcgctcgtt gaagttgctg cggcaccatt cacgcgcgcg cagtgaccgg aacttgcctg
     3961 cgaaccagag cgccactacg atgctcctcc catgcgtaga tcacttttgg cttttttcct
     4021 gctggtgccg ggcctcgccc acgcgcaagc cgccgactcc agccgcttcg tggtccttca
     4081 cggtgccgat accgtcgcga ctgagcagtt gaaacggacc gagaccgagc ttcatggcac
     4141 gttggcgttt cgcggtgcca ggacgacgga gctctatcgg gcagtgctcg ccccggacca
     4201 gacggtgccc ttggtcgagg tgacggtaca ggaggccccg gccagcagcg ccgacaaagc
     4261 ccgagttgcc cagcaggccc ggatcatttt tcggaacgac agtgtggcga tcgacgacat
     4321 cagcaatcat gggctggaaa cccgcgtact gccgacgcag tcgggcgcaa tcccttacat
     4381 caatctttcg ttcgggctac tggaacaggc gatacgacgc gcggcggtac tggggaagga
     4441 ttcggtgaaa gttgccttct tcaatctgag tggtagtgca tcacagggcg gtggcgctac
     4501 agtagtgggg acggttgctc gctttgccgt cgattctgtg tcgctggatc tgggacaggt
     4561 cgagttccgt ctgcgggtgg actcacaagg ccgactgctc gggggtggaa ttccaacaca
     4621 gcggctctcg tttacgcgtc attgatttac cccaggccgg gacggatgtc gtacacccga
     4681 cccttccact cgatccgacg tgccccacga atcaccgagc gcaagatgat atagagtcct
     4741 accagtgctc cggccggata cgccagcccg tggagcacgg ggattcccat tcccactgac
     4801 accagcatcc agaacagggc agatagcgcc acggcgacca gcgcccatcc catccagggc
     4861 accactccga agaaagccag cagcaggagg atcggcggaa gcagccagaa gagcatcgtg
     4921 ccggagagca tcatcggaac tagcgcccgc cggatgggct cgtccggaaa ggttttccgc
     4981 cccccgatgt agatattctt ggaccagcc
//
LOCUS       DASRJQ010000397         2664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1065854, whole genome shotgun sequence.
ACCESSION   DASRJQ010000397 DASRJQ010000000
VERSION     DASRJQ010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065854"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1236)
                     /locus_tag="VFB89_08920"
     CDS             complement(<1..1236)
                     /locus_tag="VFB89_08920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZM27466.1"
                     /translation="MHESEGMAGREFEDLIRQLAPQVLAALMRRYGHFDTAEDATQEA
                     LLAASQQWPSEGIPDNPRAWLITVASRRLTDMLRAEGARQRREDAVAQQMGIDDGLAP
                     PADAEASGDDTLVLLFMCCHPSLSSASQIALTLRAVGGLSTAEVARAFLVPEATMTRR
                     ITRAKQSIKSSDVPFQLPSEPSRLTVVLHVLYLIFNEGYATTSGPSLHRADLSAEAIR
                     LARMVRRMLPDDAEVTGLLALMLLTDARRPARTGPSGELIPMAEQNRLLWNSEMIAEG
                     VSLVDEAMSHGPVGPYQLQAAISALHDQAPSVEETDWPQIRALYEVLLDRSDNPIVAL
                     NHAVAVAMATNPAAGLSLVDTLAADERIAEDHRLFAVRAHLLEMAGDTAKARTAYQAA
                     AARTSSLPQKRYLHAKAARL"
     gene            complement(1279..2004)
                     /locus_tag="VFB89_08925"
     CDS             complement(1279..2004)
                     /locus_tag="VFB89_08925"
                     /inference="COORDINATES: protein motif:HMM:NF022432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HZM27467.1"
                     /translation="MTDAMLDKVRKLLAKAEDPGCSPAEAAALNDKAAELIAKYGVDR
                     ALLAAAAPDVDPVGDRNIPIYPPYALDKASLLGVVASALRCRSVRTKQRTTIGYSYGM
                     HLFGCASDLERTELLYTSLLVQASYGLAATFVPRDEPVAAFRRSWIAGFTQAVGIRLR
                     EAENRAAAAADNGGGPSMALVLADRGGRVDRRVSEVYPRLATAPPRRLAGGGRGHGYA
                     AGQKADLGGARLGRATSGSLGSP"
     gene            complement(2043..2456)
                     /locus_tag="VFB89_08930"
     CDS             complement(2043..2456)
                     /locus_tag="VFB89_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27468.1"
                     /translation="MGEQTGAARDVPSEVYQDAAAVLDSALRGDRDGVVGTFDAAVGR
                     GGLKGAYEVAWCLAATMVGESVTGFATLDYPDIERARYDARWVARFVSAYANADPPTA
                     EALFGAALADGQLSECLMTLAGSTIATLRQRGLGA"
BASE COUNT          465 a          925 c          862 g          412 t
ORIGIN      
        1 aaggcgagcg gccttggcgt gtaggtagcg cttctggggc aagctgctcg tccgtgccgc
       61 agccgcctgg taggcggtac gcgccttcgc cgtgtccccc gccatctcga gcagatgagc
      121 ccgtaccgcg aagagccgat ggtcctcggc gatccgttca tcagccgcga gcgtatccac
      181 tagggacagc ccagcggccg gattcgttgc catcgccacg gcgaccgcat ggttcaacgc
      241 cacgatcggg ttgtccgacc tgtccagcag cacctcatac agcgccctga tctggggcca
      301 gtcggtctcc tcaacggacg gggcttggtc gtgcagtgcg gagatcgccg cctgcagctg
      361 gtacggcccg accggaccgt gtgacatcgc ctcgtcaacg agcgagacgc cctcagcgat
      421 catctcggag ttccacagta gacgattctg ctccgccatt gggatgagct caccggacgg
      481 cccggtgcgg gcgggacggc gggcatcggt gagcagcatg agcgcgagca gcccggtcac
      541 ttcggcgtca tcgggcagca tccgccgtac catgcgcgcc agccggatcg cctcggcgga
      601 gaggtccgcc cggtgcaacg agggtccaga tgtcgtggca tatccctcgt tgaagatcaa
      661 atagagtacg tgtaggacca cggtgagccg ggaaggctcg gacggcagtt ggaacggcac
      721 gtcgctggac ttgatgctct gcttcgccct ggtgatgcga cgggtcatgg tcgcctccgg
      781 caccagaaac gctcgcgcga cctccgccgt actgagacct ccgaccgcac gcagagtgag
      841 cgcgatctgt gaagccgacg acagcgaggg gtgacagcac atgaagagca gtaccagcgt
      901 gtcatcccca ctcgcttcgg catcggccgg cggcgcaagc ccgtcgtcaa tccccatctg
      961 ctgggcgact gcgtcctcgc gacgttgccg agccccctcc gctcgcaaca tgtcggtgag
     1021 ccgccgggac gcgaccgtga tcagccaagc gcgcgggttg tccggaatcc cctcgcttgg
     1081 ccactgctgc gacgccgcaa gcagcgcctc ctgagtcgcg tcctccgcgg tgtcgaagtg
     1141 cccgtaccgc cgcatcaacg cggcgagcac ctgcggagcg agctgccgaa tcaggtcctc
     1201 aaactcgcgg cccgccattc cctcactctc gtgcaagcag gtagtcccta caacgccgaa
     1261 tcggcatgta ggccgacatc agggcgaacc gaggctgccc gaggtggccc ggcccaaccg
     1321 ggcgccgccg agatcggcct tctgcccggc ggcatatccg tggccccgtc caccacccgc
     1381 caggcgtcga ggcggcgccg tggccaagcg cggatatacc tctgacacgc gccggtcgac
     1441 ccgacccccg cggtcagcca ggacgagtgc catcgacggt ccgccaccgt tgtctgcggc
     1501 agccgcggct cggttctccg cttcacgtag ccggatgccc accgcttggg tgaagccggc
     1561 gatccaggat cgccgaaacg cggccaccgg ctcgtcgcgc ggcacgaagg tagccgccag
     1621 gccgtaggag gcctggacca gcagcgacgt gtagagcaac tcggtccgct cgaggtcgga
     1681 cgcacagccg aacagatgca tgccgtagga atagccgata gtcgtccgct gcttggtccg
     1741 tacgctgcgg cagcgcagcg cggaagcgac gacaccgagc aggctcgcct tgtccagcgc
     1801 gtacggcgga taaatcggaa tgttgcgatc gccgacggga tcgacatccg gggcggcggc
     1861 ggcgagtagc gcgcggtcca cgccatactt cgcgatcagt tcggcggcct tgtcattgag
     1921 cgcggccgcc tccgccggag agcatcccgg atcttcggcc ttggccagga gcttgcgaac
     1981 cttgtcgagc atcgcgtcag tcatacgaac acctgtacca gagggcaccg acaggacctg
     2041 ggttatgcgc cgagtcctcg ctgccgcagg gtggcaatcg tggaacccgc gagcgtcatc
     2101 aaacactcgg acagctgccc gtcagccagc gccgcgccga acagagcctc ggctgttggt
     2161 gggtccgcgt tagcgtaggc gctcacaaag cgagccaccc accgggcgtc gtatcgtgct
     2221 cgttcgatgt ctgggtagtc cagggtggcg aagccggtta cggactcccc gaccatcgtc
     2281 gcggccaggc accaggcgac ctcgtacgcg cccttcagcc cgcctcggcc gaccgcggcg
     2341 tcgaaggtgc ccaccactcc gtcgcggtcc ccgcgcagcg ccgagtcgag gactgcagcg
     2401 gcatcctgat acacctccga cggaacgtcc cgcgcagcac cggtctgctc acccacgtga
     2461 cgcagcgtat gcgccaaacg caaatgccgc gatgcgatgc cggagcgtta agaaaggcac
     2521 cttataccgc gcaaagcgtt aacaaggtgc ccttcctttc aaagcggcgg ccggcgaagc
     2581 aagcaggtca gccgcgcggt gcacacccgc tcgtcgtcct catcggtgat gacgatctcg
     2641 tacgtagcga cagatccgcc cagg
//
LOCUS       DASRJQ010000398         2936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2341225, whole genome shotgun sequence.
ACCESSION   DASRJQ010000398 DASRJQ010000000
VERSION     DASRJQ010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2341225"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VFB89_08935"
     CDS             complement(<1..688)
                     /locus_tag="VFB89_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HZM27469.1"
                     /translation="MPVLTYRDALNAALREEMRRDPDVFLMGEEVGVYQGAYKVSRGL
                     LEEFGPMRVVDTPITELGFAGVGVGAAMAGLRPVIEFMTWNFALLAIDQLVNSAAKMR
                     YMSGGQVGVPAVFRGPGGAALQLGAQHSQAFESYYAHIPGLKVVMPATPADAKGLLKT
                     AIRDDNPVVFIEGEMLYNTKGEVPEGEYTIPLGQAEVKKSGRDVTIVCHSKTVSVALK
                     AAEQLAGEGIE"
     gene            complement(764..1804)
                     /gene="pdhA"
                     /locus_tag="VFB89_08940"
     CDS             complement(764..1804)
                     /gene="pdhA"
                     /locus_tag="VFB89_08940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HZM27470.1"
                     /translation="MAHSTAARRARQTAKPSPSALAEEYRGWLRQMMVIRRFEEKAGE
                     AYSLGKIGGFCHLYIGQEAVAVGSIAALRPDDYIVCSYREHGHALARGMSPRTVMAEL
                     FGKAAGCSKGKGGSMHLFDAERHFMGGHGIVGGHIPLATGLAFAAKYRNTEQVAVCYF
                     GEAAVNNGAFHEALNMAALWKLPCIYICENNRYGMGTALERASAIYDISERASSYDMI
                     NEVVDGQDVLVMKNAMDRAVERARLEKAPTLLEVRTYRFMGHSMSDPIHGHYRTKEEV
                     EEHRKRDPIALWSRKLITDSLMDEPAIRTLDAEVTAEIAEAYEFADKAPDPEPGELFT
                     DVYKETGVNGER"
     gene            complement(1804..2676)
                     /gene="lipA"
                     /locus_tag="VFB89_08945"
     CDS             complement(1804..2676)
                     /gene="lipA"
                     /locus_tag="VFB89_08945"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010331747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HZM27471.1"
                     /translation="MTPARKPGWLKVKAPGGPTYLELKGLMRELGLHTVCEEARCPNI
                     GECWEHKAATFMILGDVCTRNCTYCAVAHGTPKAYDPEEPARLAEAVSRMRLEHVVIT
                     SVDRDDLPNGGAEVFAASVREIRARMPGTSVEVLIPDFKGSETALRIVMEAEPDILNH
                     NLETTDRLYRLARPGGRYDRALELLARARRLSPNALTKSGIILGLGEEWDEIVRCLRD
                     LRRSDVDIVTLGQYLRPSDAHLPIARYYTPEEFDELRAIGLALGFAHVQASPLTRSSY
                     HAWAQVDQARSARA"
BASE COUNT          514 a          948 c          906 g          568 t
ORIGIN      
        1 cttcgatccc ttcgcccgcc aactgctccg cggccttgag tgctacgctg accgtcttcg
       61 aatggcatac gatggtcacg tcgcggccgc tcttcttgac ctcggcctgg cccagcggaa
      121 tcgtgtactc cccttcgggg acttcgccct tggtgttgta cagcatctcg ccttcgatga
      181 aaacgacggg attgtcgtcc cggatggccg tcttgagcag ccccttggcg tctgccggtg
      241 tggccggcat gacgaccttc agtcccggga tgtgcgcgta gtagctctcg aacgcctgcg
      301 aatgctgcgc acccagctgg agcgctgcac cgcccggtcc ccgaaacacg gcgggcacgc
      361 caacctgtcc gcccgacatg taccgcatct tggctgcgga gttgaccagc tgatcaatgg
      421 caagaagggc gaagttccag gtcatgaact cgatcacggg ccgcaggccc gccatcgcgg
      481 ctcccacacc cacaccggcg aagccgagct cggtgatggg cgtgtccacc acccgcattg
      541 ggccgaattc ctcgagcagc cctcgactaa ccttgtacgc accctgatag accccgactt
      601 cctcgcccat gaggaagacg tcggggtcac gccgcatctc ctcgcgcaga gcggcgttca
      661 gggcgtcgcg gtaggtgaga acaggcaagg ttctttaact gcgtaaacgg taaacggtga
      721 acggtgaacg ggggctcgct aggcgggatg ccccgtttac cgtttaccgt tcaccgttca
      781 cgcctgtctc cttatagaca tcggtaaaca actctcccgg ctccgggtcc ggcgctttgt
      841 ccgcgaactc gtacgcctcg gcgatctcag ccgtgacttc ggcgtcgagc gtgcgtatcg
      901 ccggctcatc catcagactg tcggtgatga gcttgcgcga ccacagggcg atcgggtcgc
      961 gtttgcggtg ctcctcgacc tcctccttgg tacgatagtg cccgtggatg gggtcggaca
     1021 tcgagtgacc catgaaccgg taagtgcgta cctcgagcaa cgtcggggct ttttcaaggc
     1081 gggcccgctc caccgcgcga tccattgcat tcttcattac cagaacgtcc tgaccgtcta
     1141 cgacttcgtt gatcatgtca taggaggacg ctcgctcgga gatgtcgtag atcgcgctgg
     1201 cccgttccag cgccgtaccc atcccgtacc gattgttctc gcagatgtag atgcagggca
     1261 gcttccacaa ggccgccatg ttcagcgctt cgtgaaaggc gccgttgttg acggccgctt
     1321 cgccgaagta gcacaccgcg acctgctcgg tgttgcgata ctttgcggcg aaggcaagcc
     1381 cggttgcgag aggaatgtgg ccacccacaa tgccgtggcc ccccatgaag tgccgctcgg
     1441 cgtcgaagag atgcatcgag ccgcccttgc ctttcgagca gcccgcagct ttgccgaaca
     1501 gctcggccat caccgttctc ggcgacatgc cgcgcgccag cgcgtgccca tgctcgcgat
     1561 aggagcacac gatatagtcg tcgggccgaa gcgcggcgat gctcccgacc gcgactgctt
     1621 cctgaccgat gtagaggtga cagaagccgc cgatcttgcc gagactgtag gcttcgccgg
     1681 ctttttcctc gaatcggcga atgaccatca tctgacggag ccagccgcga tactcttcgg
     1741 ccagcgcact ggggctgggc ttggctgtct gccgcgcgcg ccgagcggcg gtggaatgag
     1801 ccatcaggct ctcgcgcttc gagcctgatc gacctgcgcc caggcgtgat aggaggagcg
     1861 agtcagcgga cttgcctgta cgtgggcaaa gccgagcgcc aggccaatgg cgcgaagctc
     1921 gtcgaattcc tctggggtgt aatagcgggc aatcggcagg tgcgcatcgg acggcctgag
     1981 atactggcct agggtcacga tgtccacgtc gcttctgcgc aggtcgcgca gacagcgcac
     2041 gatctcgtcc cactcttcac ccaggccgag gatgatgccc gacttggtga gggcgttggg
     2101 tgacaatcgc cgagcacggg ccaacagctc gagggctcga tcatagcggc cgccgggtcg
     2161 tgccaggcgg tacagccggt cagtggtttc gaggttgtgg ttgaggatgt caggctcggc
     2221 ttccatgaca attcggagtg ccgtttccga gcccttgaag tccggaatca gaacctcgac
     2281 cgaagtcccg ggcatccgtg cacggatttc gcggacgctg gcggcgaaga cctccgctcc
     2341 cccgttgggc aagtcgtccc gatcgaccga ggtgatcacc acgtgctcga gtctcatccg
     2401 gctcacggcc tcggcgagcc gagccggctc ctcggggtcg taggctttcg gcgtgccgtg
     2461 cgcaacagcg cagtaggtgc agttccgggt gcacacatcg cccaggatca tgaacgtggc
     2521 ggccttgtgc tcccagcatt ccccgatatt cggacagcgg gcctcttcgc aaacggtatg
     2581 aagcccgagc tcccgcatga ggcctttgag ctcgagatag gtcggtccgc ccggcgcctt
     2641 gaccttgagc cagccgggct tacgcgcagg agtcaccgcg ccggcagagg cgccgcgaga
     2701 aagctgcacc agatccagct tgggtaccgc cactactccc tccaatcgaa tcagccgctt
     2761 ttcattcaac cctcaaagct agcgccgatt gggggccgtt tctactggcg agtgatgtat
     2821 tcgctaggag aagagcatcg cacccacgat gggcacccac cgtgagttct ttgccaggcc
     2881 agagtgctgc cgttggcgtc gaaccgcaaa cgccggaagc aggccggtgg tgattc
//
LOCUS       DASRJQ010000399         9738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3720523, whole genome shotgun sequence.
ACCESSION   DASRJQ010000399 DASRJQ010000000
VERSION     DASRJQ010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 9738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9738
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3720523"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..271)
                     /locus_tag="VFB89_08950"
     CDS             complement(56..271)
                     /locus_tag="VFB89_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27472.1"
                     /translation="MGDTREMRRQLLVMIGVIIVVDAAAIAAWFYAGIRGRSAAVQVL
                     FTIVWTAATLAAVIVSMRKIMGARKGT"
     gene            complement(264..605)
                     /locus_tag="VFB89_08955"
     CDS             complement(264..605)
                     /locus_tag="VFB89_08955"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZM27473.1"
                     /translation="MDHRFTVAQAVAALPAAPDEQYTIVFRHGTLELGLYAPRGSDPQ
                     DPHTRDEVYVVLRGSGWFVNGPSRDRFGPGDVLFVPAGTVHRFEEFTDDFQVWVVFYG
                     PQGGEQLVAHG"
     gene            complement(608..850)
                     /locus_tag="VFB89_08960"
     CDS             complement(608..850)
                     /locus_tag="VFB89_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27474.1"
                     /translation="MTEPELAVRRRAAERARQVVEGNLSPDALREEFADVEDRAVAAL
                     LDLLEHRPVHGVLGGGEGAWQDYQRSLRDVIAGLSA"
     gene            complement(843..1331)
                     /locus_tag="VFB89_08965"
     CDS             complement(843..1331)
                     /locus_tag="VFB89_08965"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZM27475.1"
                     /translation="MSPATLLDLFAWDTWANQKTLSSLRPAVTAAPKAGRLAAHIAAT
                     ELLWLARIHARPSPVPVWPDVGLDETARLFADAANEWKAYLGSMTVAEESRQVWYVNT
                     KGESFRNSTNHIATHVVFHSHYHRGQIAALVRAAGHEPAYTDFIHAVRSNAFTPKEVV
                     RD"
     gene            complement(1340..1801)
                     /locus_tag="VFB89_08970"
     CDS             complement(1340..1801)
                     /locus_tag="VFB89_08970"
                     /inference="COORDINATES: protein motif:HMM:NF016130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HZM27476.1"
                     /translation="MISRRDFLAGTAGLAAVGAVVRPLLALSPAPLVTVYKTPTCQCC
                     AQWVEHLKLNGLRTEVRDLDSVDAIKDQHGVPESLRSCHTALVDGYVIEGHVPADDIQ
                     RLLEQRPKIAGLTVPGMPVSAPGMYKPGDPKTPYQVLAFQKSGTTVVYAKH"
     gene            complement(1895..2419)
                     /gene="lspA"
                     /locus_tag="VFB89_08975"
     CDS             complement(1895..2419)
                     /gene="lspA"
                     /locus_tag="VFB89_08975"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HZM27477.1"
                     /translation="MSWTPKAKAFWSLLAVLVLADCSTKRLAVYHLSPPYIPHEVMGD
                     WFRLTLAYNRGAAMGLFFGPYSRLGLSLTALFALALLGTLYRRSSAGDAWRAAALALV
                     AGGAVGNLLDRLRSARGVVDFIDVGIGDMRFWTFNMADIGITCGALLLAILLAREPGN
                     PEGSGAGDDFRDTT"
     gene            complement(2421..2720)
                     /locus_tag="VFB89_08980"
     CDS             complement(2421..2720)
                     /locus_tag="VFB89_08980"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HZM27478.1"
                     /translation="MFQIVWEFEIEPGRASEFERAYGSGGVWATFFREGEGFLGTELF
                     RSLGQRERYITIDRWSSRAAFESFKRTRTREYADIDARCESLTKAERLIAAQEDP"
     gene            complement(2745..3233)
                     /locus_tag="VFB89_08985"
     CDS             complement(2745..3233)
                     /locus_tag="VFB89_08985"
                     /inference="COORDINATES: protein motif:HMM:NF024297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZM27479.1"
                     /translation="MHIARCLGPAFLLVVVAAPARAQTSGPDRTAILAVVQRLFDGMR
                     AGDSAAVRSTFHPQALLATTAKRDGKAVVRIDTLDAFVRAVGTPHSETWDERVRTPRV
                     EMDGPLASVWAEYSFYAGGKFRHCGVDAFQIARDGDAWRIVSLMDTRRKEGCPDQPSR
                     SP"
     gene            3534..4298
                     /locus_tag="VFB89_08990"
     CDS             3534..4298
                     /locus_tag="VFB89_08990"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HZM27480.1"
                     /translation="MADRLSYSRTWKPYAVALVIATSGCSAGGPPADRATGVQPMAQP
                     TPASPTSPTPVPASAPKPASRPRPAPKPQIRWADSTVLEAAVTNRLGYRPLAAVLARR
                     TRQPEVAERAALAVVRESRRLKMSPSLLAAVLLIENRALDTAAVSSAGAVGLMQVMPV
                     HAGSYGCANTELRELDTNICHGARLLRLYVVRSPSLQVALRRYNGCVRGRVTPRCSRY
                     PVRVMATASSVRREILARAAEDGVIVPGLAARSLSD"
     gene            4375..5112
                     /locus_tag="VFB89_08995"
     CDS             4375..5112
                     /locus_tag="VFB89_08995"
                     /inference="COORDINATES: protein motif:HMM:NF024221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HZM27481.1"
                     /translation="MATTSNPVLARATFAPTGEVGTMPMTLGGTIAKTLVLLGILTVT
                     ATFSWLQAGAGLAEPDKLGTLFLVGIVGGFGVALYTVFRPQHAPVTAPIYAALEGLLL
                     GALSAILNLRYKGLPVQAVVLTVATLGVMLVSYRLGIIRATERFKTIVISCTFAVAAF
                     YIIALVMAMLGLYIPFLHEGGPIGIGFSLFVTGLAAFNLILDFDLIEQGVGRAPGYME
                     WYAAFGLVVTLVWLYLEILRLLRKLRR"
     gene            complement(5123..5701)
                     /locus_tag="VFB89_09000"
     CDS             complement(5123..5701)
                     /locus_tag="VFB89_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZM27482.1"
                     /translation="MQPHPPVERLTPSIRTDTMPFDLSEAVLVLRRTPSTLRALLAEL
                     SDNWLHGREGPNTWSPFDVVGHLIDGEETDWIPRARIILAGGPNPVFEPFDRFRHLRL
                     NAGRPIGDLLERFARLRANNIATLEGFRLSARDLERPGIHPELGAVTMGQLLATWVAH
                     DLGHVAQVARVMAKQYGAEVGPWREYLPVLTR"
     gene            complement(5708..6232)
                     /locus_tag="VFB89_09005"
     CDS             complement(5708..6232)
                     /locus_tag="VFB89_09005"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZM27483.1"
                     /translation="MSGEFPATDKPQTGPTVQIRPARADDLPTLSSLTVRLAEFPIPM
                     WRTGAQVIDAERTAVTRALTAGSPDAPILVAEDTAGRVLGFAYLETHQDYFTGLFHAH
                     VSILVVAGTAEGRGVGRALLSAAEAWARERGDPFITLNVFAQNVRARALYERLGYGAE
                     TLRYVKPLDSADAG"
     gene            complement(6229..6678)
                     /locus_tag="VFB89_09010"
     CDS             complement(6229..6678)
                     /locus_tag="VFB89_09010"
                     /inference="COORDINATES: protein motif:HMM:NF022479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HZM27484.1"
                     /translation="MTPELQAQSDSAVSAVVHVIQLSVAPVFLLSGVGTILVVLTNRL
                     SRIVDRTRLLEQTTAAPTPTEAIKVQLDSLHRRAHLINWALSLATLCALLICGVIGAL
                     FIGAFFRINASKLVAVAFVIAMAALVGALLNFLREIFLATSGLRRGH"
     gene            complement(6698..7261)
                     /locus_tag="VFB89_09015"
     CDS             complement(6698..7261)
                     /locus_tag="VFB89_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27485.1"
                     /translation="MLIGLGTLAFLAGGPPCRAQTVVRDSALPPADSIRLSPDPNARR
                     NWDSVPPAVRPDTAPAPRVVRPDSANKPAAAGAAPGAQAKPPARPAPDPYPKGVCTAD
                     VPESGGDAPDILMVTFVSRAKPADREAALKTVNGTLVAPDPDDPRIWYVHVASDGNEF
                     VLRSIADRLIRARSVSEVAPVQCPPRQ"
     gene            complement(7315..7566)
                     /locus_tag="VFB89_09020"
     CDS             complement(7315..7566)
                     /locus_tag="VFB89_09020"
                     /inference="COORDINATES: protein motif:HMM:NF040444.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HZM27486.1"
                     /translation="MVQGGLMAKGRSTRRTSKGRRYGKAAGKRVESAMRRRKRGTLRS
                     GRGGKGGKVKSRKQAIAIGLSEARKKGAKVPRRRKKSSK"
     gene            complement(7636..8172)
                     /locus_tag="VFB89_09025"
     CDS             complement(7636..8172)
                     /locus_tag="VFB89_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZM27487.1"
                     /translation="MSLSTTGDGDAASPPISIRPLNGRLEAEACARMMGASEPWVTLR
                     RSYARSLETLTEPDKEVYIACDDRNIAGFLILDLRGPLRGYIQTVCVEPSRRGHGVGT
                     KLIRWAEARIEQESPNVFLCVSSFNPAARRLYERLGYQLVGTLTDYLVPGHDEYLFRK
                     TTGSWEAFRAASAAARDK"
     gene            complement(8179..8643)
                     /locus_tag="VFB89_09030"
     CDS             complement(8179..8643)
                     /locus_tag="VFB89_09030"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZM27488.1"
                     /translation="MPGKVSPIPPGRPQLTPYLIVKNGAAAIEFYQKAFGAKELGRFD
                     DHGRIGHAELEIGDSRIMLSDEYPDMGILSPLSLGGSGVNLHLYVENVDAVARQAITA
                     GATVVRPVEDQFYGDRGGKLQDPFGHIWSLASRIEDVSLEEAHKRAEALGAS"
     gene            complement(8751..9155)
                     /locus_tag="VFB89_09035"
     CDS             complement(8751..9155)
                     /locus_tag="VFB89_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27489.1"
                     /translation="MRGAGGTPGGLPSFLAGAAMLVVGGYLLLARVTVTSGYWALWSY
                     NAFGLSLVPLLIGIGVLFFNGRSALGWALTAIGTLIILAGIVANLHLYFQPTSLFDTL
                     LMLGLLAAGLGLIARSLRPQGPGTARDDSWAS"
BASE COUNT         1786 a         3118 c         3128 g         1706 t
ORIGIN      
        1 cagtgaacgg ggggctccgc tcgcaggaac ccccgtttac cgtttaccgt ttcgttcacg
       61 ttccttttct agcgcccatg atttttcgca tcgacactat caccgccgcc agcgtagccg
      121 cagtccaaac gatcgtgaag agaacctgaa ccgccgcgct ccggccccgg attccggcat
      181 agaaccacgc cgcgatcgcg gccgcatcga cgacgatgat caccccgatc atcacgagca
      241 actggcgtcg catctcccgc gtgtcaccca tgggccacca actgctcgcc gccctggggc
      301 ccgtaaaaca caacccagac ctgaaaatcg tcggtaaact cttcgaagcg gtgtaccgtc
      361 cccgcgggaa cgaagagcac gtcgcccgga ccgaagcgat cgcgggaagg gccgttcacg
      421 aaccaccctg atccgcgcag cacgacgtag acctcgtcgc gggtgtgcgg gtcctgggga
      481 tcgctgcctc tcggtgcgta gagcccgagc tccagcgttc cgtgccggaa gacgatggtg
      541 tactgctcgt ccggagcagc cggtaatgct gcgacggcct gagcaacggt gaagcgatga
      601 tccacggtta cgcggacagc ccggcgatta cgtcgcgcag cgagcgctga tagtcctgcc
      661 acgcgccttc gccgccaccc agcacgccgt gcaccggccg atgttccagc aagtcgagca
      721 gcgcggcaac ggcgcgatct tcgacatcgg caaattcctc gcgcaacgca tccgggctca
      781 agtttccttc caccacctgc ctggcacgtt cggcggcgcg gcgcctgacc gcgagctccg
      841 gctcagtcac gaacaacctc cttgggtgtg aaagcgttgc tacgcacggc gtgaatgaaa
      901 tcggtatagg cgggctcgtg tccggcggcg cgaaccaggg cggcgatctg gccgcggtga
      961 tagtgcgagt ggaacaccac gtgggttgcg atgtggttcg tggaattgcg aaacgattcg
     1021 cccttggtgt tgacgtacca tacttggcgt gattcttcgg cgacggtcat gctgccgaga
     1081 taggctttcc actcgttggc ggcatcggcg aacagcctgg ccgtttcgtc gaggccgacg
     1141 tccggccaga ccggaaccgg cgacggccgg gcgtgaatgc gagccagcca gagaagctcc
     1201 gttgccgcga tgtgcgcagc gagccggccg gccttgggtg cagcggtcac ggcaggccgt
     1261 agcgagctca aggtcttctg attagcccag gtgtcccagg cgaagaggtc gagcaatgtg
     1321 gcaggagaca cggaagcgat cagtgcttcg cgtacacgac cgtggtgccg gacttctgga
     1381 atgcgagcac ctggtacgga gtcttggggt cgcccggctt gtacatgccg ggagcgctca
     1441 ccggcatgcc aggtaccgtt aaccctgcga ttttggggcg ctgctccagg agccgttgaa
     1501 tgtcgtcagc cggaacgtgg ccctcgatta catagccgtc caccagggcc gtatggcagg
     1561 agcgaagtga ctcgggtacg ccgtgctggt ccttgatggc gtccaccgaa tccagatcac
     1621 gaacttcggt gcggagcccg ttcagtttga gatgctcgac ccactgagcg cagcactgac
     1681 aggtaggagt cttgtaaacg gtcacgagtg gggccggcga cagggccaac aagggccgaa
     1741 ccacggctcc caccgcggcc aggccggcgg tgcccgcaag aaagtcgcga cgcgaaatca
     1801 tggacgctcc ttcggagtcg ccagagggat agggacgtta tcacgggatg gcctggaaat
     1861 gccagcattc accgtctgac cagcgaaggc tctctcaagt ggtgtccctg aaatcatcgc
     1921 cagcgccgct cccctcgggg tttcccggct cgcgagccag gagaatagcg aggagcagtg
     1981 cgccgcaggt aatgccgatg tcggccatgt tgaaggtcca gaatcgcatg tccccgatgc
     2041 cgacgtcgat aaagtccacc acgccacgcg ccgagcgcag ccgatccagc aggttgccta
     2101 cagcaccgcc ggcaaccagc gccagcgcgg cggcgcgcca cgcatcaccc gccgagctgc
     2161 gacggtacag cgtccccagc agggcgagcg cgaacagagc ggtcaacgac aacccaaggc
     2221 gggaatatgg gccgaagaac aggcccatgg ccgctccacg attgtaggcg agggtcaggc
     2281 ggaaccagtc gcccatgacc tcgtgcggga tgtacggcgg cgaaagatgg tagaccgcga
     2341 gccgcttggt gctgcagtcg gccagcacca gcactgcaag cagcgaccag aacgccttgg
     2401 ccttgggagt ccaactcacg tcagggatcc tcttgggccg cgattaaccg ctcggccttg
     2461 gtgagcgact cgcatcgggc atcaatgtcg gcgtactcgc gggttcgcgt ccgcttgaag
     2521 ctctcgaagg ccgcgcgcga gctccatcgg tcaatggtga tatagcgctc ccgctgcccc
     2581 aggcttcgga acaattcggt tcccagaaag ccctcgccct cgcgaaagaa tgttgcccat
     2641 acgcccccgc tgccgtacgc ccgctcgaat tcactcgcgc ggccgggctc gatctcgaac
     2701 tcccagacga tctggaacat gaggaagcac gagtgacaga ccgatcaggg cgaccgcgag
     2761 ggctgatcgg ggcagccttc ctttcgccga gtatccatca gagacacgat gcgccaggca
     2821 tcaccgtcgc gagcgatctg aaaagcatcg acgccgcaat gcctgaactt tccgccggca
     2881 taaaatgaat actccgccca gacggacgcc aacggcccgt ccatttcgac ccgaggagtg
     2941 cggacccgct cgtcccacgt ttccgagtgg ggcgtgccta cggcacgaac gaacgcatcc
     3001 agcgtatcga tcctgaccac ggcctttccg tcgcgtttcg ctgtcgtcgc cagcaaggct
     3061 tgcgggtgaa aggtactgcg gacggcggca ctgtctccgg cacgcatccc gtcgaacagg
     3121 cgctgaacaa cggccagaat tgcggtccgg tcgggcccgg aagtctgggc ccgagccggg
     3181 gcggccacaa caactagaag aaaggccggg ccgagacatc gagcaatgtg cattgaaaac
     3241 gctcgcggga agagaggttg cggttgatcg gaactcccat tgtcaccgtc gccggccgaa
     3301 caacgaccga gggcaatgta acaatgctga ttccgggcga cgcgcgccaa cagttgcact
     3361 ccgagcgcgt atcgggggcg tgagtgctgc tgatagcgtg acttccgaaa cgcgagtcga
     3421 tgtcgctctg tgatcacgag tgtcggcttt tcgcgcattg ccactttccg aacagttgtg
     3481 aggcccaggt accggtcccg atactgcgcg gcacgcagga aacgggagga cttatggccg
     3541 accgactgtc atacagccgg acgtggaagc cgtacgccgt ggcgctggtc atcgccacgt
     3601 cgggatgctc ggccggcggc ccgccggcgg accgtgcaac cggcgtccag cccatggccc
     3661 agccaactcc cgcgtcgcca acgtcaccga cgccggtccc tgcatccgcg cccaagcctg
     3721 cctcgcggcc gcggcccgcg ccgaagccac agattcggtg ggcggacagc acggtattgg
     3781 aagcggccgt taccaaccgg ttgggttatc gtcccctggc cgcggttctg gcgcgccgaa
     3841 cccgccagcc ggaagtagcg gagcgcgccg cgttagcagt agtgcgcgaa agccgccggc
     3901 tcaaaatgag tccgagcctg cttgccgccg ttctgctgat cgagaaccga gctctcgaca
     3961 ccgcggccgt gagcagcgcg ggagcggtgg gcctgatgca ggtgatgcct gttcatgccg
     4021 gaagttacgg ttgcgccaac accgagctgc gagagctcga caccaacatc tgccacggcg
     4081 ctcgactgct acgtctctac gtagttcgaa gcccgtcgct ccaagttgca ctgcgccggt
     4141 acaacggctg cgtgcgtggc agggtcactc cgcgctgctc gaggtatccg gtccgggtga
     4201 tggcgaccgc ttcgagcgtg cgacgcgaga tactggcgcg cgcggctgaa gacggcgtca
     4261 tcgtgcccgg tctcgcagcc cggagcttgt ccgactgagg ctccgccctg aattgccgcc
     4321 ggggcatttt tcctgcaggt tcggcggcac cttcgcaact ctcgaggaag attgatggcc
     4381 acgacatcca atccggtgct ggcccgggcc acgttcgcgc cgacgggtga agtcgggacg
     4441 atgccgatga cgcttggcgg gacgattgcc aagacattgg ttctgttggg gatcctgacc
     4501 gtcaccgcca cgttctcctg gttgcaggcg ggcgccggtc tggccgagcc tgacaagctg
     4561 ggcaccctct ttctggtcgg catcgtcggc gggttcgggg tggcgcttta cacggtcttt
     4621 cgccctcagc acgccccggt caccgcgccg atctatgccg cgctcgaggg tttgctgctg
     4681 ggcgcgctgt cggccatcct gaacctgcgt tacaaggggc tgccggtgca ggccgtggtg
     4741 ctcaccgtcg ccacgctcgg cgtgatgctc gtgtcgtacc gacttggcat tattcgcgca
     4801 accgagcgct tcaagaccat cgtcatatcc tgcacgttcg cggtggccgc gttttacatc
     4861 atcgcgctgg tgatggcgat gctcggtctc tacatccctt tcctgcatga gggcgggccg
     4921 atcggcattg gattcagtct tttcgtaacc ggccttgccg ctttcaatct catcctggat
     4981 ttcgacctga tcgaacaggg cgtgggacgc gcgccggggt acatggagtg gtacgcggcc
     5041 ttcgggctgg tcgtgaccct ggtctggctc tatctcgaaa tccttcgcct tctgagaaag
     5101 ctccggcgct agtcgcaccg ggtcagcgcg taagtaccgg caggtattca cgccacggtc
     5161 ccacctccgc tccatactgc tttgccatga cgcgcgcgac ctgagcgaca tgacccaggt
     5221 cgtgcgccac ccaggtggcc aggagctggc ccatcgtgac ggcacccagc tcgggatgga
     5281 ttccgggtcg ctcgaggtcg cgggccgaca gcctgaagcc ttccagcgtc gcaatgttgt
     5341 tggctcgcag tcgggcaaat cgctcgagca ggtcaccgat gggcctgcct gcgttgagtc
     5401 gcagatgacg aaaccggtcg aacggctcga acaccgggtt cggtcccccc gccagaatga
     5461 ttctcgcccg cggaatccag tcggtctctt cgccgtcgat caggtgtccg accacgtcga
     5521 acggactcca ggtattgggc ccctcgcgcc cgtgaagcca gttatcggac agctccgcga
     5581 ggagggcgcg caacgtgctt ggcgtccggc gcaagaccag gacggcctcg gaaaggtcga
     5641 agggcatggt gtccgtacgg attgaagggg tgagtcgttc cacgggaggg tggggttgca
     5701 tccgaactta tcctgcgtcg gccgagtcca ggggcttcac gtatcgcagc gtctccgcgc
     5761 cgtatcccag gcgctcgtac agtgcgcgtg cccgcacgtt ctgagcaaag acgttgagtg
     5821 ttatgaatgg atcgcctcgc tctcgggccc aggcttcggc ggctgagaga agagcgcgcc
     5881 ctaccccgcg gccttctgcc gtccccgcca cgacgagaat cgacacgtgc gcatggaaga
     5941 gaccggtgaa gtaatcctgg tgggtctcga gataggcgaa gccgagcact cgaccggccg
     6001 tgtcttcggc gacaagaatc ggtgcatccg gggaaccagc tgtcaacgct cgagtgacgg
     6061 cagtgcgctc agcatcgatc acttgagcgc ccgtgcgcca catgggtatg gggaattcgg
     6121 ccagccgaac cgttaacgac gagagggtcg gaaggtcgtc ggctcgggcc ggccggatct
     6181 gaactgtcgg gccggtttgg ggcttatcgg ttgccgggaa ctccccgctc aatggcctct
     6241 cctcaggcca ctggttgcga ggaagatctc gcggaggaaa ttgagcaggg cgccgaccaa
     6301 agctgccatg gcgatcacga aagcaaccgc gacgagcttg gaggcattga ttcgaaagaa
     6361 ggcgccgatg aagagagctc caatgacgcc gcagatcaaa agggcgcaca gggttgccag
     6421 gctcagggcc cagttgatca agtgcgcccg gcgatgcagg ctgtcgagct gaactttgat
     6481 tgcttcggtc ggtgtggggg cagccgtcgt ctgctccagc aagcgcgtgc gatcgacgat
     6541 tcgggaaagc cgattggtga ggaccacgag aatggtgccg acgccggaca gcaaaaagac
     6601 cggagcgacg gacagctgaa tgacgtggac caccgcgctg acggcggaat cgctctgtgc
     6661 ctgtagctcc ggcgtcatct agggcttcgg acgccttcta ctggcgcgga gggcactgaa
     6721 ccggagccac ctcgctcacc gagcgggcgc ggatcaggcg atccgcgatg ctgcgcagca
     6781 cgaactcgtt tccatcgctg gccacgtgga cgtaccagat ccgcggatcg tcgggatccg
     6841 gggcgaccaa cgttccgttg accgtcttca aggcggcctc gcggtcggcg ggcttggcgc
     6901 gcgagacgaa ggtgaccatc aaaatgtcgg gggcgtcgcc gcccgactcg ggcacatccg
     6961 cggtgcaaac acccttggga tagggatcgg gtgccggccg cgcaggtggt ttcgcttggg
     7021 ccccgggagc ggccccggcc gctgccggct tgttagcgct atcgggtcgg acgactcgag
     7081 gagcaggtgc ggtgtccggt cgaaccgccg gaggaacgct gtcccagttc cgccgagcat
     7141 ttggatcagg cgaaagcctg atgctgtcgg ccggcgggag cgcactgtcg cgaacgacgg
     7201 tctgcgcgcg acatggcggc ccgcccgcca ggaaggcgag cgtaccgagc ccgatcagca
     7261 agcccgatcg tgaaaccatg tccatcaaga tacgcgatcg gggcgcgcca ggctctactt
     7321 gctggacttc ttacgccgcc gggggacctt ggcgcccttc ttcctcgctt ccgaaagccc
     7381 gatcgcgatc gcctgcttgc ggctctttac cttgccgccc ttgccgccgc ggccgctccg
     7441 aagagttccc cgctttcgcc tccgcatcgc gctctcgacg cgcttccccg cggctttgcc
     7501 atacctgcga ccttttgatg tccgcctggt ggaccgcccc ttcgccataa gtcctccttg
     7561 gaccatgtga ttgcaacggt tccaagctgc gaattgacag gggttcggta ccgtgacgcg
     7621 ggttacggag tcgagtcatt tgtcgcgagc cgcagcgctc gcagcccgaa atgcctccca
     7681 ggagccggtc gtcttccgga atagatactc gtcgtgtccg ggcaccaggt aatccgtgag
     7741 cgtgcccacc aactggtaac ccaggcgctc gtacaaccga cgcgccgccg ggttgaacga
     7801 ggacacgcaa agaaacacgt tggggctctc ctgttcgatg cgtgcttccg cccaacggat
     7861 cagcttggtg cctacgccat gcccgcggcg ggatggttcg acacataccg tctggatgta
     7921 gcctcggagg ggccctctga ggtcaaggat cagaaatccc gcgatgttcc ggtcgtcaca
     7981 agcgatgtaa acctccttat ccggctccgt cagggtttcg agggaccgag catacgagcg
     8041 ccgcaaggtg acccagggct ccgacgcccc catcatgcgg gcgcaggctt ccgcttcgag
     8101 ccgtccgttg agcgggcgaa tcgagattgg cggcgatgca gcgtcgccat cgccggtcgt
     8161 cgacaggctc acgtcggctc aggacgcgcc gagcgcttcg gctcgcttgt gggcttcctc
     8221 gagggacaca tcttcgatac ggctggcaag cgaccagatg tgcccgaatg ggtcctgtag
     8281 cttgcccccg cgatcaccgt aaaactgatc ttcgaccggg cgaactacgg tcgccccggc
     8341 cgttatcgcc tgccgcgcga cagcgtccac gttctcgacg tacaggtgga gattcacacc
     8401 cgatccgccc aggctgagcg ggctcagaat gcccatgtcg ggatactcgt ccgacaacat
     8461 gatgcgggag tcgccgattt cgagctcggc atgcccgatc cggccgtgat cgtcgaaacg
     8521 gcctagctcc ttggccccga acgccttctg gtagaactcg atggcggcag caccgttctt
     8581 gacaatgaga tagggggtga gttgagggcg ccccggcgga atggggctca ctttacctgg
     8641 catgatgcga tctcctgata atcggtcctg cgacgcgtag cgtgaaaccc atgctgctgc
     8701 gtcgcgcaat gcttcatgct tcacctgcga tccggagtat gcaacccgga tcaggaagcc
     8761 cagctgtcgt ctcgcgcagt gcctgggcct tggggtcgta gggatcgggc aatgagcccc
     8821 aggccggcgg ccaggagacc caacatgagg agggtatcga acaacgatgt tggctgaaag
     8881 tacagatgca ggttggccac gatgccggcc agaatgatga gagttcctat tgcggtcagc
     8941 gcccacccga gggctgaccg gccgttgaag aagagcacac cgatgccaat cagaagcggc
     9001 acaagcgaga gcccaaacgc attgtagctc cacaacgccc aatagccgct ggtcacggtt
     9061 actcgcgcaa gcagcagata tccgcccacc acgagcatgg ctgcaccggc gagaaacgac
     9121 gggagcccgc caggtgttcc gcccgcccct ctcatgcacc accgagcggc atgtgctcct
     9181 ccgccatagg ctggactcga gccggtatct gcaaagggtg tcgcgccttg actggcggcg
     9241 caagggcggt caactgagcg gtggctttcg ccgcggcggt ctccccgacc gctgtacaat
     9301 gtctccctcc gcggtcattt gtgggcagct cccatcgact tgccggagtc gcccggccac
     9361 ccgattgaat tgcgcgacga atgcctttct acggtcccac agggcgaagc aggcgcggtg
     9421 catcgcagac tcggagaagc ggaagaaagg atcggtgtcg tccgcaatga agtccggtgt
     9481 gactaccgca tcggcatccg gccgtatggc ctcgttgcac aacgcacaaa cagcgctgcc
     9541 ccctcgaact cgactcagcg actcggcgcg cgcgcgattg cggtggttgc gggtgcccac
     9601 ggtcaaggat cgtttccgag cacagccacc agggaatcca ggaacggtgc ctggccgggc
     9661 aggatacgtc gccgcgcctc agccagatcg aaccaggagg ctcgatccac ttcaggaaaa
     9721 gtgcgcatcc gtcccgag
//
LOCUS       DASRJQ010000400         3865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1145862, whole genome shotgun sequence.
ACCESSION   DASRJQ010000400 DASRJQ010000000
VERSION     DASRJQ010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145862"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1629)
                     /locus_tag="VFB89_09040"
     CDS             complement(<1..1629)
                     /locus_tag="VFB89_09040"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZM27490.1"
                     /translation="MQELHSRLAIALADRYEIERGIGSGGAAIVYLARDLKHGRQVAL
                     KVLRTDVGASLGADRFLREIRIAARLNHPHILAVHDSGEAGGLLYYVMPYVEGESLRE
                     RLKREGALPLEDALRFAREVADALAYAHSQGVVHRDVKPGNILLVGGHAVVADFGIAM
                     AAGGDTDSLAELGGPIGTPNYMSPEQSGARGPVDGRSDQYSLGCVLYEMLTGKPPFDG
                     PTARTVMKRHASEPPPSVRSSRPLVPPSVDLAVRRALAKLPADRFATLHQFAQALEAA
                     ETPRRMPARAMAMPVAMALGLLAVGIGAATMLRRAPVETGSAAAGVLVVPMTPQDSGA
                     RLPARSIVNILSSAIDWLPGIQTIDGLPLADSLARSPVRQLLANARRQEARYVLAGSI
                     VSEGDGRIAQVDLYSAGGGDRIVRSQSPVSGDGVGPALALGRAAMAAARGLAEREHMI
                     LGSRAMLFDATGSTIALGYLLEGQDRFWRAQFDAAASAFRAAIEADSSCALAYHRLSV
                     AHVWRHDYSAALAAANQALVRSPGMAPRWRALLDA"
     gene            complement(1643..>3865)
                     /locus_tag="VFB89_09045"
     CDS             complement(1643..>3865)
                     /locus_tag="VFB89_09045"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM27491.1"
                     /translation="AHVSRAPEPVNTVRTTVPADLAALVMRCLEKRPADRWQSAEDLL
                     RALEVVSTPGTGTAATGALTTVVTPVPAQVPTGTGRFRTARGVGAGILVLVLAFLGLK
                     GAGVLSGRSLVDRGVFRERESILVAEFQSPPADSALGGAVTEAFRVDLAQSPKLTVVQ
                     PEYVQQVLLRMQRDPRVPLDAALAREVAIRDGIKAFVAGDVVHAGTGFVISARLIATQ
                     SGEALVALRETADDSSRILPAIDRLSRGMRERIGGSLGTVRASERLEDVTTPSLEALR
                     KYSQALRADGQGDTERAISLLREAVELDTGFAMAYRKLGVELGNSGGRRSQQIDAFTK
                     AYNHRDRLTDRERYLTQAAYESYVNGDRDRAITAYRSLLDLSPNDPFALNNLSIALSN
                     LRNFGEAAELARRAIRAAPDGSLFYGNLITAQVGLGQVDSAEATLRFLRSRMPGDPYG
                     AFIEASFLASSGAYDSARAIVQKIQQARRESPNWQTLTHGQLAILALTEGHLDQADGY
                     LQTAGEISESQGLPERALLFDFARAGIDLWYRGQPQRARQKMDAALQRRPLSRFDPAD
                     RPYLELALLYAEAGRPDRAKALVSEFDKVAPEGFRRLSGSERHGVLGSIALAERRPLD
                     AINEYRQQDDGDCDICMLAPLGRAYDDAGQVDSALAVYERYVDSPYMYRLFPDAVWLG
                     RTYYRLGELYESRGDRAKAAENYAKFVRLWKDADVELRPKVLDAKSRLKALTGEHATT
                     "
BASE COUNT          649 a         1313 c         1263 g          640 t
ORIGIN      
        1 agcgtccagc agggctcgcc agcgcggggc catgccgggc gaacggacca gcgcctggtt
       61 ggcggccgca agagccgccg aatagtcgtg ccgccagaca tgcgcgacgc tcaaccggtg
      121 atacgccagc gcgcaagacg aatcggcctc gatcgccgcg cgaaacgcgc tagccgccgc
      181 atcgaactgg gctcgccaga accggtcctg tccctccagc aagtagccca gcgcgatggt
      241 ggacccggtc gcgtcgaaca acatcgctcg cgagcccaga atcatgtgct cccgctccgc
      301 gagtccgcgg gctgcggcca ttgccgcacg cccgagcgcg agcgcgggcc cgacgccgtc
      361 gccggagacc ggtgactggc ttcgcacgat ccggtcgccc ccgccggcgg agtatagatc
      421 cacttgggca attcggccgt ccccctccga tacgatggag cctgccagga cgtagcgcgc
      481 ttcctgtcgt cgagcgttcg cgagcagctg gcgcacgggc gatcgggcga gcgagtcggc
      541 cagaggaagc ccgtcgatgg tctgaattcc cggcagccag tcgattgcgc tcgagaggat
      601 gttgacgatc gatcgcgccg ggagtctcgc gccgctgtcc tgcggcgtca tcggcactac
      661 caggacaccc gccgccgccg acccggtctc gaccggcgcg cggcgaagca tggttgcggc
      721 gccgatcccg actgcaagca gcccaagagc catcgcgacc ggcattgcca tggcgcgggc
      781 cggcatgcgc cgaggtgttt cggctgcttc cagggcctga gcgaactggt gcagggtggc
      841 gaatcgatcg gccggcagtt tggccagcgc tcgccgcaca gcgagatcga cggaaggggg
      901 aaccagggga cggcttgatc tgaccgacgg cggaggctcc gatgcgtggc gtttcatcac
      961 ggtccgagcg gtcgggccgt cgaacggtgg cttccccgtc agcatctcgt acagcacgca
     1021 ccccagactg tactggtcac ttcgcccgtc caccggtccc cgggcgccgc tctgctcggg
     1081 gctcatgtag ttcggcgtcc cgatcggccc acccagctct gccagcgagt cggtgtcacc
     1141 tcccgcagcc atcgcgatgc cgaagtccgc caccacagcg tgccccccga ccagcaaaat
     1201 gtttccgggc ttgacgtcgc ggtggactac gccctgcgaa tgcgcgtacg ccaacgcgtc
     1261 cgcgacttcc cgggcaaagc ggagtgcgtc ctccagtggc aaggcgccct cgcgcttgag
     1321 acgttcgcgc aacgactccc cttcgacata cggcatgacg tagtagagta ggccaccggc
     1381 ttcgccggag tcgtgtactg ctaggatgtg aggatgattc agacgcgcag cgatgcggat
     1441 ttcacgaaga aagcggtcag cgccgagtga tgcacccaca tcagtgcgaa gcactttgag
     1501 cgcgacctga cgtccgtgct tgagatcacg tgcgagatag acgatcgccg cacccccgct
     1561 gccgatgccg cgctcgatct cgtaccgatc cgccagcgcg atggcaagcc gggaatgaag
     1621 ctcttgcacg ggggtgaacc ccctatgtgg tggcgtgctc gccggtcagt gctttgagcc
     1681 ggctcttggc atcgagcacc ttggggcgca gttcgacgtc cgcgtccttc cagagccgca
     1741 cgaacttggc gtagttctcc gctgctttgg ctcggtcgcc gcgactctcg tacaactcac
     1801 ccagtctgta ataggtccgc cccagccaaa ccgcgtcggg gaacagacgg tacatgtagg
     1861 gactgtccac atatcgctcg tatacggcta atgcggagtc gacctgtccc gcatcgtcgt
     1921 acgcccggcc cagcggggcc agcatgcaga tgtcgcagtc accgtcgtcc tgctgccggt
     1981 attcgttgat tgcgtccagc ggccgccgct cggcgagggc gatagacccc agtactccat
     2041 gccgctcgga cccggacaag cgtcggaagc cctcgggcgc aaccttgtcg aattcggaga
     2101 ccaaagcctt cgcccgatcc gggcgccccg cttcggcgta gagcagggca agctcgaggt
     2161 acggacggtc ggccggatcg aatctggaga gcggccggcg ctggagcgcc gcgtccatct
     2221 tctgccgcgc ccgctggggc tgcccgcggt accacaggtc gatgcccgcc cgcgcgaagt
     2281 cgaacagcag tgcccgttct ggcaggccct gcgactcact gatctccccg gcggtctgca
     2341 ggtatccgtc ggcctgatcc agatgccctt cggtgagcgc aaggattgcg agctggccgt
     2401 gggtaagcgt ctgccagttc ggactctcgc gccgggcctg ctgaatcttt tgcacgatgg
     2461 cacgggcgct gtcatacgcg ccgctcgatg cgagaaagct ggcctcgatg aacgcgccgt
     2521 aaggatcacc gggcatcctg ctccgcagga agcgcagcgt cgcttcggcg ctgtccactt
     2581 ggcccaggcc tacctgcgcc gtaatgagat tgccatagaa cagtgacccg tcgggcgcgg
     2641 cgcgaatggc gcgtctggcg agctctgcgg cttccccgaa gttccgcagg ttgctgaggg
     2701 cgatactgag gttattcagg gcaaaggggt cgttggggct cagatcgagc agcgaccgat
     2761 aggcggtgat ggcccggtcg cggtcgccgt tcacgtagct ctcataagcc gcctgggtca
     2821 ggtagcgctc acggtcggtc agccgatcgc ggtggttgta agctttggtg aaggcatcga
     2881 tctgctggct tcggcgcccg ccgctgttgc ccaactctac gccgagcttg cgatatgcca
     2941 tcgcgaaccc ggtatccagc tcgaccgctt cccgtaacaa cgagatcgcg cgttcggtat
     3001 cgccttggcc gtctgcccga agagcctgcg agtacttgcg caacgcctcg agcgagggcg
     3061 tggtgacgtc ttccagtcgc tcgcttgcgc gaaccgtccc cagggaccca ccgatccgct
     3121 cgcgcatgcc tcggctcagc cgatcgatgg cgggcaggat acgactcgag tcgtcggccg
     3181 tctcccggag cgccaccaat gcctcacccg actgggtagc aatcagcctg gcggagatga
     3241 cgaagcccgt gccagcgtgg actacatcgc ctgcgacaaa ggccttgatg ccgtcgcgga
     3301 tcgccacttc acgcgccagc gctgcatcga gcgggacacg gggatcccgc tgcatccgca
     3361 gcagcacctg ctggacatac tccggctgga ccacggtgag cttgggcgac tgggcaaggt
     3421 ccacccggaa cgcttcagtt acggcgccgc ccagagcgga gtctgccggc ggactctgga
     3481 actccgcgac caggatcgac tcgcgctccc ggaacactcc ccggtccacc agagagcggc
     3541 cggacagcac accagcgcct ttcagaccca ggaaggccag gaccagcacg agaataccag
     3601 ccccgactcc tcgagcggtt cggaatcgcc ctgtgcccgt ggggacttgg gcgggaacag
     3661 gagtcaccac cgtagtcaat gcgccggttg cggccgttcc ggtaccgggc gtcgatacta
     3721 cctccaatgc tcggagcagg tcttccgcgc tctgccagcg gtccgccggg cgcttttcca
     3781 ggcagcgcat gacgagagca gccagatcgg ccggcacagt tgtgcggacg gtattgaccg
     3841 gctcgggggc gcggctcacg tgggc
//
LOCUS       DASRJQ010000401         2473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1952095, whole genome shotgun sequence.
ACCESSION   DASRJQ010000401 DASRJQ010000000
VERSION     DASRJQ010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1952095"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="VFB89_09050"
     CDS             complement(<1..880)
                     /locus_tag="VFB89_09050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HZM27492.1"
                     /translation="MSRKPRLPERDKPWVMRTYAGHSSAAASNALFRRNLTKGQTGLS
                     VAFDLPTQTGYDPDDVLARGEVGRVGVPVTHLGDARALFHDLDLGAANTSMTINATAM
                     WLLALYVTVAQEQGAPLPALAGTTQNDIIKEYLSRGTYIFPPAPSLRLTTDMIAWTVT
                     NCPKWNPVNICSYHLQEAGATPVQEVGFALATAIAVLDSVRDSGQLPPERMSDVVARI
                     SFFVNAGVRFIEEMCKMRAFGALWDELVAKRYGVTDAAARRFRYGVQVNSLGLTEAQP
                     ENNVQRIVLETLGVTLS"
     gene            1202..2065
                     /locus_tag="VFB89_09055"
     CDS             1202..2065
                     /locus_tag="VFB89_09055"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZM27493.1"
                     /translation="MVDEAIVVRGLRKAYGEKLAVDGLDLTVQRGEVFALLGPNGAGK
                     TTTVEIMEGYRRRDAGEVRVLGADPAKASTAWRGRVGIVLQGTGEFEELQVREVVRLF
                     AGYYPDAEDPDEVIDRVGLAEKRAALTHTLSGGQKRRLDVALGIVGRPELLFLDEPTT
                     GFDPEARREFWELIRDLAKGGTTILLTTHYLEEAEQLADRVGVIAAGRMIAVATPAEL
                     GGRADSAATVSWQSPDGPRSILTATPTEYVSRLSSELGGGEIPGLTVTRPTLEDVYLT
                     MIEATSRSGDA"
     gene            2062..>2473
                     /locus_tag="VFB89_09060"
     CDS             2062..>2473
                     /locus_tag="VFB89_09060"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM27494.1"
                     /translation="MSTTTLSSAPAVGIDRLVRLGLRQIGNEIKQFLRSKQQVVFTMG
                     FPIIMLVIFGSIFSGDLGGGVKFTQYFVTGMIASGLMVTGFQALAIQIPIERDRGVLK
                     RLAGTPMPRSVYFIGKVGMVLAIAAAETVLLLAVA"
BASE COUNT          410 a          802 c          842 g          419 t
ORIGIN      
        1 gggacagcgt caccccgagc gtctccagga cgatccgctg cacgttgttt tcgggctgtg
       61 cctccgtgag cccgagcgag ttcacctgca cgccgtaccg gaaccggcgg gccgccgcgt
      121 cggtgacgcc gtaacgcttc gcgaccagct cgtcccagag cgcgccgaac gcgcgcatct
      181 tgcacatctc ctcgataaac cgcacgccgg cgttgacgaa gaaggagatc cgggcgacca
      241 catcgctcat ccgctccggt ggaagctgac cgctgtcgcg gacggagtcg agcacggcta
      301 tggcggtggc caatgcgaag cccacttcct gcaccggggt cgctccggcc tcctgcagat
      361 ggtaggagca aatgttgact ggattccact tcggacagtt ggtgaccgtc cacgcgatca
      421 tgtccgtggt gagccgcaac gacggcgcgg gcgggaagat gtacgtgccg cgggacaggt
      481 actccttgat gatgtcgttc tgcgtggtgc ccgccaatgc cggcagcggc gcgccctgct
      541 cctgtgccac ggtcacgtaa agcgcaagca gccacatcgc cgtggcattg atggtcatcg
      601 acgtgtttgc ggctcccagg tcgagatcgt ggaagagcgc gcgcgcgtcg ccgaggtggg
      661 tcaccgggac gccgacacgg ccgacctcac cgcgggctag aacgtcatcg gggtcataac
      721 cggtctgcgt gggcaggtcg aaggcgacgc tgagaccggt ctggcccttg gtcaggttgc
      781 gccggaacag cgcgttgctc gcagcggcgg acgagtggcc ggcgtacgtc cgcatcaccc
      841 agggcttgtc ccgctcgggc aagcgtggct ttctggacat cgcagcctcc tcaggaaccc
      901 tgcgcgggca cccgacacct gaacggtcga tcagccaacc cgtttgcggc agtctacttc
      961 tgagtaaccc agacagatcc ggtgaattac ctcactgagt atctgggcct cgctccgctc
     1021 cggccggctc gcgtggcgac gcgcccccat gtgcggcctt ccctcgtcgc tcgcttcctc
     1081 gttcctcggt cgctcgactc cttagtccag gccgcacggg cgcttaccgc cacgactcgc
     1141 gagtggggtc acccggcgga gcggagcgag gcccagaaac ttgtgccgct agattcacgg
     1201 catggttgac gaggcgatcg tcgttcgtgg gctgcggaag gcgtacggcg agaagcttgc
     1261 cgtcgatggc ttggacctca ccgtccaacg gggcgaggtt ttcgcgctgc tcgggccgaa
     1321 cggcgccggg aaaacgacca cggtcgaaat catggagggc taccggcgcc gcgatgcggg
     1381 tgaggttcga gtgctcggcg ctgaccctgc caaggcgagc accgcgtggc ggggccgggt
     1441 cggcatcgtg ctgcagggca cgggggagtt cgaggagcta caggttcgag aggtggtaag
     1501 gctcttcgcc ggctactacc cggatgccga ggatccggac gaggtcatcg accgggtcgg
     1561 cctggcggag aaacgcgcag cgctcacaca cacgctctcg ggcggccaga aacgccgact
     1621 cgacgttgcg ctgggcatcg tcggaagacc agagctgctg ttcctggacg agccaacgac
     1681 cgggttcgac ccggaggcgc gccgcgagtt ctgggaactc atccgcgatc tggcgaaggg
     1741 cggcacgacg atcctgttga ccacccacta tctggaggag gccgagcagc tcgcggaccg
     1801 agtgggtgtg atcgcggccg gccgcatgat cgcggtggcc acgccagcgg agctcggcgg
     1861 ccgggccgac agcgccgcca cggtcagttg gcagagtccc gatggtccac gctcgatcct
     1921 taccgccacg ccgaccgagt acgtgagccg gctcagctcc gaactcggcg gcggtgagat
     1981 ccccggcctg accgtcaccc ggcccacgct ggaggacgtg tacctcacga tgatcgaagc
     2041 gacttccagg agtggcgacg catgagcacc accaccctca gctcggctcc tgccgtcgga
     2101 atcgaccggc tggtacggct cggtctacgc cagatcggga acgagatcaa gcagttcctg
     2161 cgcagcaaac agcaggtcgt gttcaccatg ggcttcccga tcatcatgct cgtgatcttc
     2221 ggttcgatct tctcgggtga cctgggcggg ggcgtgaagt tcacgcagta cttcgtgacc
     2281 ggaatgatcg cgtccggcct catggtgacc gggttccagg cgctagcgat ccagattccg
     2341 atcgagcggg accggggtgt tctcaagcgc ttagccggaa cgccgatgcc gcgctcggtc
     2401 tacttcatcg gcaaggttgg catggtgctc gcgatcgcgg cggcggagac ggtgctcctg
     2461 ctggcagtcg ccg
//
LOCUS       DASRJQ010000402         2806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2256103, whole genome shotgun sequence.
ACCESSION   DASRJQ010000402 DASRJQ010000000
VERSION     DASRJQ010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2256103"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="VFB89_09065"
     CDS             <1..724
                     /locus_tag="VFB89_09065"
                     /inference="COORDINATES: protein motif:HMM:NF016612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HZM27495.1"
                     /translation="AQLAVVRVGGLLLAAVPAEPTTNAGWELSDAVRSGAVEVGAPPE
                     RVAILGLANGYIHYVATEEEYRAQHYEGASTIYGPATARVLAREFHEMAVTIRGPAAP
                     SPPDEQLPITVYPGPPRQLLPRPGIGARVERAVLRVECDPHEIIAEWRDLAPADLLPT
                     DDWLLTFWNRAEGAPDEPVAWDDRPDVEVRPLAAMKDGGWRWQARWRPAIPGRYQFVL
                     PARAGLPEVAGPVCASGTGRGE"
     gene            complement(739..>2806)
                     /locus_tag="VFB89_09070"
     CDS             complement(739..>2806)
                     /locus_tag="VFB89_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27496.1"
                     /translation="RALWRPSTPWLVPVALAAAAALVLLSIFRSAASHDTHPAGRTDF
                     AVVPFSGPTGDDTIGQRLARYVGNELEWFPGWRLTSVPTSFAWWASAAPNRRALEAPA
                     ALGARFYAEGELLPGERTVRVAVRDSSGAPYYRLTVTGEPSDLLGWGSAIADSLVRIL
                     FPQRIDEFRDVSTRGSRNVQAYTELFAGQEAFRIDDWAGAEAHYRRALELDPTFAEAA
                     WKLALVNRWRDRSFDAEWRRLYQLHRSQLPELQQLIIAAQLGPDLPARFAELEAAVRQ
                     YPRSTEGVLVEADELFHRGPLAGVPLDTAVQLFVDVSRREPYMTAYEHIAWAEIRHGR
                     EAEARRALQELNRSRDAASVAEARQRARLLALVYDFRFRPWRGRLKVRWLGWRADSTT
                     LAALAQYARWGLLFDVPELQRQLGELLISSGTTLGMRAQGYEARGIALMTEGRQRAAL
                     PDFDSAASLFGTPEVAIERAEWRLLPGVLGLPPADSASTGLARSTLAAIDGPQGARAA
                     WALAVDATTSGDSVSLARWRAKLDAVGRGDATTRRLSRLVEALARARAGDVADALALT
                     DSLLLYDSAALAQAPFARALLYLRRGEWLAATGASDQAASTWLWHESSDFEGWLNHGV
                     QAGEVDGVAGVLARLRLAELAAKRGNATVSCAEATRVKQLWADADPSYRDLVGRAASL
                     AAACHH"
BASE COUNT          491 a          928 c          965 g          422 t
ORIGIN      
        1 tgcgcagctc gcggtcgtgc gggtgggggg actcctcctg gccgcggtgc cggcggagcc
       61 tacgacgaat gccggctggg agctcagcga cgcggtgaga agtggtgccg tcgaggtggg
      121 ggcgccgccg gagcgtgtgg cgatccttgg actggcgaac ggctatatcc actatgtcgc
      181 cactgaagag gagtaccggg ctcagcacta cgagggtgct tcgacgatct acgggccggc
      241 cacggctcgt gtgcttgccc gcgagtttca tgaaatggca gttacgattc gcgggcccgc
      301 tgcccccagc ccgccagacg aacaactacc gatcacggtc tatccgggtc ctccgcgaca
      361 actgcttccc cgacctggca tcggagcccg ggtcgagcga gcagtgctga gggttgaatg
      421 cgaccctcac gaaatcatcg ccgaatggcg cgaccttgcc cccgctgacc tgctgccgac
      481 cgacgattgg ctcctcactt tctggaaccg cgctgaaggt gcccccgacg aaccggtcgc
      541 ctgggacgac cgtcccgatg tcgaagtccg gccgctcgcc gcgatgaagg atggaggctg
      601 gcgctggcag gctcgctggc ggccggcgat cccgggacgc tatcagttcg tgctgcccgc
      661 cagggctggc ttgccggagg ttgctggacc cgtatgcgcg agcgggactg gacgagggga
      721 atgacagtca agtcgatttc agtgatggca agccgccgcg agcgaggctg ctcgccccac
      781 caagtcgcga tatgacgggt cggcatcagc ccacagctgc ttgactcggg ttgcctcagc
      841 acaggataca gtcgcgttgc cgcgcttggc cgcaagctct gcgaggcgga gtcgggcaag
      901 cacgcccgcg accccatcca cttctccggc ttgcaccccg tgattgagcc aaccttcaaa
      961 gtccgaactc tcgtgccaga gccaggtgct agccgcctga tcgctggccc cggtggcggc
     1021 gagccactcg ccacgccgca ggtagagcaa cgcacgcgca aagggagcct gggccagtgc
     1081 ggcgctgtcg tagaggagca acgaatcggt gagggcaaga gcgtcggcga catctccagc
     1141 gcgtgcccga gccagcgcct cgaccaaccg cgacaaccgc ctggtggttg cgtcgccgcg
     1201 gccgactgcg tcgagcttcg cacgccagcg ggcgagtgag acagaatcgc cgctcgtggt
     1261 ggcgtccacc gcgagggccc acgccgcacg cgcaccctgc ggcccgtcga tggcagccag
     1321 cgtgctgcgg gccaggccag tcgaagcgga atcggccggg gggaggccga ggactccggg
     1381 cagcaatcgc cattcggctc gctcgattgc gacctcgggc gtaccaaaca ggctcgccgc
     1441 cgagtcgaag tcagggagcg cggcgcgctg gcggccttca gtcatgagcg cgataccccg
     1501 ggcctcgtaa ccctgagccc gcatgccgag ggtggtgccg ctgctgatga ggagctcgcc
     1561 gagttgacgt tgcaactccg gaacatcaaa gagcagcccc cagcgcgcgt actgcgcgag
     1621 agccgcgagc gttgttgaat cggcccgcca gccgagccat cggaccttca acctgccccg
     1681 ccagggacgg aaccggaaat cgtaaacaag agcaaggagg cgggcccgtt gtcgcgcttc
     1741 ggcgacactg gctgcgtccc ggctccggtt cagttcttgg agagctcgac gggcctccgc
     1801 ctcgcggccg tgtcgaatct cagcccaagc gatgtgctcg taagccgtca tgtacggctc
     1861 ccggcgtgag acgtcgacga acagctggac cgcggtgtcg agggggacac cggcgagagg
     1921 gccacgatga aacagctcgt ccgcttcgac cagcacgccc tccgtgctgc gcgggtactg
     1981 gcgtaccgcc gcctcgagct ccgcgaaacg ggccggcagg tcgggcccaa gctgcgcggc
     2041 aatgatcagc tgctggagct ccgggagctg gcttcggtgc agctggtaga gacggcgcca
     2101 ctcggcgtcg aaggatcggt ctcgccatcg atttaccaat gcaagtttcc acgccgcttc
     2161 ggcaaaagtg gggtcgagct ccaaagctct ccggtaatgg gcctcagcac cggcccagtc
     2221 gtcgatccgg aacgcctcct gccccgcgaa aagctcggtg tatgcctgga cattgcgact
     2281 gccacgggtt gacacgtcgc ggaactcgtc gatgcgttgt ggaaagagga tgcggacgag
     2341 ggaatcggcg atcgcgctac cccatcccag caggtcgctt ggctctccag tgaccgtaag
     2401 ccgatagtac ggcgcgccgc tcgagtcgcg aaccgcgacc cgaaccgtgc gttcgcccgg
     2461 tagcaactct ccttccgcgt agaaccgggc accgagcgcg gccggcgctt ccagcgcgcg
     2521 acggtttggc gccgcggacg cccaccaagc gaagctggtt ggcaccgatg taagtcgcca
     2581 gcctgggaac cactccagct cgtttcccac gtagcgggca agccgctgac cgatggtgtc
     2641 atcgccggtc ggaccgctga agggaacgac agcaaagtcg gtgcggccag ccgggtgggt
     2701 gtcgtgtgac gcggcggaac ggaagatgga cagaaggaca agcgccgcgg cggcggccag
     2761 cgcaactgga acgagccacg gcgtcgacgg gcgccacagc gcgcgg
//
LOCUS       DASRJQ010000403         3053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_803964, whole genome shotgun sequence.
ACCESSION   DASRJQ010000403 DASRJQ010000000
VERSION     DASRJQ010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803964"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VFB89_09075"
     CDS             <1..441
                     /locus_tag="VFB89_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27497.1"
                     /translation="ERAGFTERLGHYLAQPAPKKAFVVQFGRKTMKPTHLLLGDPIEA
                     DAKGLPKDLPLALHYIELLARIGALHTAPVATKVLARVLKDCEENGVWNPKGLRAAPK
                     PLSHLTYHVYPLQPDNKAPEDKLVDVTFRLALIAKLLGWSLEYV"
     gene            528..1214
                     /locus_tag="VFB89_09080"
     CDS             528..1214
                     /locus_tag="VFB89_09080"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZM27498.1"
                     /translation="MPRLDLTPFGFTPTESLVYEVLLTAGPGTGYAIARAAGLARANA
                     YSALEGLVSKGAARADGGRPRRYRPEDTNALIARIANDHGQALDRLSADLEGLAAPTI
                     PTLVEIDSPRAALQLISHDVARAEKAVALLAPPEAYPLLAPTLRRPASTGVELTLLAT
                     GPVNLSFAPVAVVDSGPEWPGTPLICVVDQKNAVIAYRQGNDVHGHWSTAPAIIAAAR
                     TAIGYFGRSE"
     gene            complement(1311..>3053)
                     /gene="lon"
                     /locus_tag="VFB89_09085"
     CDS             complement(1311..>3053)
                     /gene="lon"
                     /locus_tag="VFB89_09085"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HZM27499.1"
                     /translation="RELGRLERIGRESMESQVIRTYLENVAELPWNIRTEDHLDLPEA
                     SRILEEDHYALQDVKDRVLEFLAVRQLRDASKRAETDGRTGETPVSGNGEAAKPSPEA
                     TLADGSAGSSSKPTRLPGQEDDKVAKGPILLFVGPPGVGKTSIAKSIARAMGRKYVRI
                     SLGGARDEADIRGHRRTYVGAMPGRIIQGMKQAGTKNPVFLLDEVDKLGVSFQGDPAA
                     ALLEVLDPAQNDSFVDHYLGVAFDLSEVLFIATANFVQNIPGPLLDRMEMVEFSGYTE
                     REKQQIARQYLIPRQFQENGVAPAQLEITDDALSEVIAGYTRESGVRQLEREIGRLAR
                     KVARKIAAREVEQVVVDKPMVDDLLGRPKVHPEKAGRDDQVGVATGMYYTPTGGDIMF
                     VEASTMRGKGELVLTGQLGDVMKESARAAWTYARSHAAALQIPDEMFERDIHVHVPAG
                     AIPKDGPSAGVTMASAIVSAMSGRSVRPDVAMTGEITLRGRVLPIGGVKEKVLGAERA
                     GITTILLPKENEADLEDLPKEVRDRLEIHMVSELGEVLALTLRGASFREGRLLFGDEK
                     NPRDVVPLSAGFRH"
BASE COUNT          550 a         1040 c          900 g          563 t
ORIGIN      
        1 gagcgggccg ggttcacgga gagactgggc cactatctgg cgcagcctgc gcctaagaag
       61 gcattcgtcg tacagttcgg ccgcaagacg atgaagccga cgcacctgct gctgggtgat
      121 cccatcgaag ccgacgccaa ggggctcccg aaggatctcc cgttggcgtt gcactacatc
      181 gagctgctcg cccggattgg cgcgcttcat actgcgccgg tcgcgaccaa ggtattggca
      241 agggtgctca aggactgcga ggaaaacggg gtgtggaacc ccaagggcct tcgcgccgct
      301 cccaagcctc tcagccatct cacctaccac gtctatccgc tgcagccgga caacaaggca
      361 cctgaggaca agctggtgga cgtgactttt cggctcgcgt tgatcgccaa gctgctcggt
      421 tggtcgctgg aatacgtcta gccaaagccg ttaggcagtg gccattagcc gttagctgtg
      481 gccgacggac actagctttc attcccgtag ccatccaaac tactcccatg ccgcgcctgg
      541 acctcacacc gttcggcttc accccgacgg aaagcctggt ctatgaggtg ttgctgactg
      601 ccggcccggg taccggctac gcaattgctc gtgccgcggg gctggcgcgg gccaatgctt
      661 acagcgcgct cgagggtctg gtgagcaagg gcgcagctcg agcagacggc gggcgacccc
      721 gccgctatcg ccccgaagac accaacgcgc tgatcgctcg cattgccaac gaccacgggc
      781 aagcactgga tcggctgagc gctgacctgg aaggcttggc agccccgacc ataccgacac
      841 tggtcgagat cgactcacct cgagcggcgc tgcaactcat ctctcacgac gttgctcggg
      901 cggaaaaggc ggtggcgctc ctcgctccgc ccgaagcgta ccccctgctc gctccaacac
      961 tcaggcgccc cgcatcaacc ggcgtcgagc ttaccctcct cgcaaccggc ccggtgaacc
     1021 tgtcgtttgc accggtagcg gtggtcgaca gcggcccgga gtggccggga acccccctca
     1081 tctgcgtggt ggatcagaag aacgcagtga tcgcttaccg tcaggggaac gacgtgcacg
     1141 gccattggag cacggcgccg gcaataattg cggcggctcg gaccgctatt gggtacttcg
     1201 gccggtcgga gtaacctgaa cagcgtgaac ggtaaacagt gaacagtgaa cggggggtcc
     1261 ggtgatctgg gagcccccgc ttaccgttca ccgtttaccg ttcactgctt ttagtgtctg
     1321 aatcccgctg ataacggaac tacgtctctc ggattcttct cgtctccaaa cagcaatcgg
     1381 ccttcccgga acgacgctcc ccttaaggtc aaggcaagca cctcgccgag ctcgctcacc
     1441 atgtggatct caagcctgtc ccggacttcc ttgggcaagt cctcgaggtc cgcttcgttc
     1501 tccttgggaa gcagaatcgt cgtgatcccg gcgcgctcag cgccaagaac cttttccttc
     1561 acgccgccga tgggaagcac ccgaccccgc agcgtgatct cgccggtcat tgccacgtcg
     1621 ggccgaaccg accggccgct catggccgac acgatcgcgg atgccatggt cacgcctgcc
     1681 gacgggccgt ccttcggaat ggccccagca ggcacgtgga cgtggatgtc ccgctcgaac
     1741 atctcgtcgg gaatctgaag cgccgcagcg tgggaccggg cgtaagtcca ggcggcccgg
     1801 gcagactcct tcatcacgtc acccagctgg ccggtcagga ccagctctcc cttgccccgc
     1861 atggtgctcg cctcgacaaa catgatgtcg ccgcccgtcg gggtgtaata catgcctgtc
     1921 gcgacgccaa cctgatcgtc ccgaccggcc ttttcgggat gaaccttggg ccgacccagg
     1981 agatcgtcta ccatcggctt gtccaccacc acctgctcga cctccctggc cgcgatcttt
     2041 cgcgccacct tgcgggccaa acggccgatc tcgcgttcga gctgacgcac gcccgactca
     2101 cgggtgtaac ccgcgatcac ttccgagagc gcatcgtcgg tgatctcgag ctgcgccggt
     2161 gcgacgccgt tctcctggaa ctggcgcggg atcagatact gccgggcgat ctgctgcttc
     2221 tcccgctccg tgtagcccga gaactccacc atctccatcc ggtcaagcag cggaccaggg
     2281 atgttctgaa cgaagttcgc agtcgcgatg aacaggacct cgctcagatc gaaagccacg
     2341 ccgaggtagt ggtctacgaa cgaatcgttc tgcgccgggt ccagcacctc gagcaaggcc
     2401 gccgccgggt ctccctggaa cgagacaccc agcttgtcta cctcatccag caggaagacc
     2461 gggttcttgg tcccagcctg cttcatgccc tggatgatcc ggccgggcat agccccgacg
     2521 taggtacgcc ggtgaccccg gatgtctgcc tcgtcacgcg cgccgcccag agaaattcgg
     2581 acgtatttac gccccatcgc ccgcgcgatc gacttggcaa tcgaggtctt tcccacgccg
     2641 ggaggtccga cgaagagcag gatcggcccc ttggcgactt tgtcgtcttc ctgacccggc
     2701 aaacgcgtcg gcttggatga agagccggcc gatccgtcgg caagggtcgc ttccggagac
     2761 ggtttcgcgg cttctccatt gcccgacacc ggtgtttcgc cagtccgacc gtcagtctcc
     2821 gcccgtttac tggcgtcccg gagctgacgc acggccagga actcgagcac ccgatccttc
     2881 acatcctgca aggcgtagtg atcttcctcg agaatccggg aggcctcggg gaggtcgaga
     2941 tggtcctctg tcctgatgtt ccaaggcagc tccgcaacgt tctccagata ggtcctgatg
     3001 acctgcgact ccattgactc acggccgatc cgctccaggc gacccagctc acg
//
LOCUS       DASRJQ010000404         6831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_463351, whole genome shotgun sequence.
ACCESSION   DASRJQ010000404 DASRJQ010000000
VERSION     DASRJQ010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6831
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463351"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..1143)
                     /locus_tag="VFB89_09090"
     CDS             complement(184..1143)
                     /locus_tag="VFB89_09090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZM27500.1"
                     /translation="MSRMGVVVVSFNAREDLLACLRSIRQAAASPPPTVLVDNGSTDG
                     GPDLVRLQLPEVEVVIDPANRGYGAAANLGARRHDWPYLLLLNSDTVLAAGAVDALSD
                     YLDAHPRVAIVGPRLLAPDGALEQSCHPPLGTIRSVLEKSALGRAIARLPAIGASWAL
                     LNWAHDRARPVPWVTGAALAIRRSPFEAVGGFDEGYFMYAEEADLCWRFREAGWEVHF
                     APVTDVVHRGAASTAPLRGAMALQKVRSAIRFYRRHYGPLRAALLVLGIRAAMLLRWC
                     RDALRLRVAGDPLTRSRLAEDMAVWQKAVRRPEADPLAAVRPG"
     gene            1383..2099
                     /locus_tag="VFB89_09095"
     CDS             1383..2099
                     /locus_tag="VFB89_09095"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZM27501.1"
                     /translation="MSEFARQVRWLSSRGYQAIDLDTLLRARTSGDALPARPVLITFD
                     DGFQSCADHAVPVLQRYGFTATFFLVASLVGDTSRWMTEAKVTLPLMSWERARALARA
                     GFQFGSHTLTHPRLPELDATRRRVELWDSRRRLEDELSRPVLHFAYPYGAYDADVRAS
                     VADAGYATACGTRSGLSEPGEDLLALRRVTVHGHDSLLDFACRLRTGLAAGERLGHVI
                     KGVARRLTGRAGPDGDRGFG"
     gene            2200..3123
                     /locus_tag="VFB89_09100"
     CDS             2200..3123
                     /locus_tag="VFB89_09100"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZM27502.1"
                     /translation="MATELVPAPAPVLQPAAPAARGISIIIPAFNEAATIAQVVRAAR
                     AHCPDAEVVVVDDASTDGTADEAAGAGARVIRRPYNLGNGAGVKTGIRAASGDVILII
                     DGDGQHDPADIPRLLAHVGTYDLVIGERSRSSQQNAVRWLGNVVLNRLGTYLVGIEMR
                     DLTSGFRAMRRDVIAEFLHLLPNRFSWPTTSALAFAKAGYHVRFVPITVHKRTAGRST
                     QELLPNGIKFTLIILRIATLFSPLRIFFPIFVLLEALAVTAYVWSIAEGGPWLHLPPS
                     TVMFFLGGIVLFLFGLISEQIASLRFKGPER"
     gene            3183..4130
                     /locus_tag="VFB89_09105"
     CDS             3183..4130
                     /locus_tag="VFB89_09105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HZM27503.1"
                     /translation="MLAYFLATTDIGSIVSNLRGLRWVPFLLSLALYPLLVAVKAWRW
                     NLVVSDLGLEAPPLGHSIRLYMIGMFLSSTTPGQSGDFVKAWYLRDRGLPLAASLFSV
                     LVDRLLDFLVMALLSLVGLVAFVHLFPQQARGAIEVATVGFAVAIALVTPALMDRRSR
                     NWLMSRAVRLVPAKVGGRIERWRNQFAALDIRPGLVAGLFAGTLCATTVTMVRLWLLF
                     RAMEAAIPLAAMVTSMALISNLQSLPISFSGVGVRDAVLIPVLAYYGYGAEKALALSA
                     LVLLLNIENILLGYLVSLRHPLGPGTSTRPPTRFPPVTA"
     gene            complement(4132..5691)
                     /locus_tag="VFB89_09110"
     CDS             complement(4132..5691)
                     /locus_tag="VFB89_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27504.1"
                     /translation="MAADRDPRPDSHVPDVDAAGLRRRDHLRFLAVAVGALGLCALYA
                     WRGYFSSDFWEHAAVVRELSVRPLAPRHPLLAVDAPHAYASPYHLAVGLAARVTGAAP
                     TTMLAIAGLVNMVLLVLAIRRFLLRLPHGDAAAPYALLFIIFLWGKDVWMWSGFFHIG
                     MLGYNAPYPSTFAAAAMFLCLSVLMDALDSGRTGLFAAISLLVAVCMLTHPPTGLVLF
                     AGLAALFLARVARRFWITAGLLFGAVSAGVALALTWPYFPVLKLFADQPPEFHSWSGV
                     FYQGVVGQIWPAILALPVLLWRLRDDRRDPLVLLVALLAVIYAIGGITGRYGLGRTIA
                     YIVVFIQIALAIAAVGWESRVAKGRSWLVPSGSVVAALALFAQNKPPLPRIIRVDRQV
                     WPEAKAILGPVRPGDVVLADSRTSYLVPALSGGRVVAWRHPVYWIPDQAERRKAQDQF
                     FGNATDQERHAVIARYHVQWILLDRREVLLGAEEERRLLGLGCVIADRGALVLIEVAD
                     RCVAPASASAA"
     gene            complement(5643..>6831)
                     /locus_tag="VFB89_09115"
     CDS             complement(5643..>6831)
                     /locus_tag="VFB89_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27505.1"
                     /translation="FNQYHDCSVLQMISNADDHRWAAAVGPLMYNRNRGETDRCPTLR
                     TLVTEGPNTASYLVYRYTRYWHGYNPISAALLWVMDLGNVRTVLKLTVYAALALLVIA
                     TGLHQRLLLAVSASIALTGALFWGVPYFGQSLTHGPGDAFVILGIVGFLALRKRAGSM
                     SMLVPLCAVYGAGVAYLEFLTGLLPTAAGLLVPTVYVVERLRPEPENDPVRARRLALA
                     ALLAFALGALLTVAIKQALAVVIFGPEAVRTFAEYLHRYINPSPGASLKHFGETWSSP
                     KDPLLVSSAKAVEAVLSQGYVLTYGSHTGAIVLYAASALAWLIAGFIAVRRRVRWATS
                     DLLGFAAGGAIVLAWILAFQTHSTIHKWWMVRTLLVPLSFGWSALAWQLIAIRVPTRT
                     SPT"
BASE COUNT         1246 a         2188 c         2270 g         1127 t
ORIGIN      
        1 gaggggctcg ggcccgcgaa ggtgacgagc gtgacgtcgt ggcggcgggc cagcgtcgcg
       61 agctcgccga agatgaccac tgcgccgccc gcggtcgagg cggggtgagg cagcagaggt
      121 gccgccatca ggatgcgcat aggattcgga atgctgggtg ccggcgtgcg gccgtgaacg
      181 cgatcacccc ggccgcaccg cggcgagcgg gtctgcctcg ggacggcgga cggctttctg
      241 ccagacagcc atgtcttcgg cgagccgcga acgcgtgagc gggtcaccgg cgacgcggag
      301 ccgcaatgcg tcgcggcacc aacggaggag catcgccgcg cggatcccca gtaccagcag
      361 ggctgcgcgc agcgggccat agtggcgtcg gtaaaaccgg atagcgctgc gcaccttttg
      421 cagtgccata gctccgcgaa gtggcgcggt gctcgccgcg ccgcgatgga cgacatcggt
      481 aaccggcgcg aagtgcacct cccacccagc ctcgcgaaag cgccaacaaa ggtcggcctc
      541 ttccgcatac atgaagtagc cctcgtcaaa gccgccgacg gcctcgaacg ggcttcgacg
      601 aatagccagc gcggctcccg taacccaggg gactgggcgc gcccggtcgt gggcccagtt
      661 gagcaaggcc cagctcgcac cgatggccgg gaggcgtgcg attgcacggc ccagagcgct
      721 tttctcgagg acggaccgaa tcgttcccag gggtggatgg caagactgct ccaacgctcc
      781 gtcgggggcc agaaggcgcg gacccacgat ggcaacccgc ggatgcgcgt caaggtagtc
      841 gctgagcgcg tccaccgctc ccgcggcgag cacggtgtcg ctgttcagaa ggagcaggta
      901 gggccaatcg tgtcgcctgg ctccaaggtt tgccgccgct ccatagccac gatttgctgg
      961 gtcgatcaca acctccacct cggggagctg caggcgcacg agatcggggc cgccatcggt
     1021 cgatccgttg tccacgagca cggtcggggg gggagaagca gctgcctggc gaatcgatcg
     1081 gagacacgcc agcaggtctt cgcgcgcgtt gaagctgacg actaccactc ccatgcgcga
     1141 catgcaggct cgttggtggg gaacgggcgg taagctagcg ggccaccgga atgaggtaca
     1201 ggcgctgcgc ttgacagccg ggcgaggatt cgggcagctt cggaccggcg gtcgcacgag
     1261 tcgagaggct gagatcgctc ttcccagtgg aggcttcgat tcgcaattcc gcagtcccta
     1321 tcctcgctta ccacgaggta tcacccgatc cgtacccgcc cttccgccga tacacgctga
     1381 aggtgagcga gttcgctcgc caagtgcggt ggctgtcgtc gcgcggatac caggcgatcg
     1441 acctggacac gctgctccgg gcccgaacga gcggagacgc gcttccggcc cggccggtgc
     1501 tgatcacctt cgacgatggc tttcaatcct gcgcggatca tgccgtgccg gttctccaga
     1561 gatacggatt tacggcgacg ttctttctcg tcgcgagcct ggtgggagat accagccgtt
     1621 ggatgacgga ggcgaaagtg acgctgccgc tgatgagttg ggagcgtgcc cgggcgctcg
     1681 caagggcggg ctttcagttc ggcagtcaca cgctcacgca cccgcgcctt ccggagctcg
     1741 acgcgacgcg tcgtcgagtc gaactttggg actcgcgccg ccggctggag gacgagctga
     1801 gtcgtccggt cctgcacttc gcctatccct acggcgccta tgatgcggac gtgcgggcca
     1861 gcgtggccga cgcgggctat gcgacggcct gcggcacgcg ctcggggttg tccgagccgg
     1921 gcgaggatct tctggcgctc cgccgggtga ccgtgcacgg gcatgacagc ttgctcgact
     1981 ttgcctgccg tctgcggacc ggtttagcgg ccggagaaag gttagggcat gtcatcaaag
     2041 gcgtcgcgcg gcgccttacg ggccgagccg ggccggacgg cgaccgcggg tttggttaga
     2101 ccgtctcgcc tacgggtcca gtcccgcagc ggcttggttc ttgccaagtt tgagacggaa
     2161 ctcgtcgcca gtcgtatcgc cccgcccgga ggatgacccg tggctactga gcttgtcccc
     2221 gcaccagcgc ccgtcctcca gccggcggcc ccggcagccc gcggcatcag catcatcatt
     2281 cccgcgttca acgaagctgc gaccatcgct caggttgttc gggccgcgcg cgcacactgc
     2341 cctgacgccg aggtggtggt cgtggatgac gcgtcgaccg acggcaccgc cgatgaggct
     2401 gcgggagcag gtgcccgcgt catccggcga ccctacaacc tgggaaacgg ggccggcgtg
     2461 aagaccggca tacgagccgc gagcggcgat gtcattttga tcatcgatgg cgacggccaa
     2521 cacgatcctg cggacatccc gcgactcctg gcacatgtag gcacctacga cctggtcatc
     2581 ggcgagcgga gccgcagcag ccagcagaac gcggttcgct ggctcggaaa cgtggtgctg
     2641 aaccggctgg gcacctacct ggtggggatc gagatgcgcg acctcacctc cggcttccga
     2701 gccatgcgcc gggatgtgat cgccgagttc ctccacctgc tgccgaatcg attttcctgg
     2761 ccaaccacga gtgccctggc gtttgccaag gccggatacc acgtccggtt cgtgccgatc
     2821 accgtacaca agaggaccgc cggccgcagc acccaggagc tgctacccaa cggcataaag
     2881 ttcacgctga tcatcctgcg catcgctaca ctgttcagcc cgctgcggat cttcttcccc
     2941 atctttgtac tgctcgaagc tctggcggtg accgcctacg tctggtcgat cgccgaggga
     3001 gggccctggc tgcacctacc gccttcgacc gtaatgttct ttctgggcgg cattgtgctc
     3061 tttctgttcg gcctcatctc cgagcagatc gccagtttgc gctttaaagg acccgagcgc
     3121 tgacgcccag tcgagctcgg aaccgatatg cccgctggct cctgcgcctg atcgggcctg
     3181 cgatcctcgc gtacttcctg gcgaccaccg acatcggcag catcgtttcc aacctgcgcg
     3241 gcctgcgctg ggtgccgttc ctgctttcgc tcgcgctgta tccactcctc gtcgccgtga
     3301 aggcctggcg gtggaacctg gtcgtgagcg atctgggcct ggaagcgccg ccgctgggtc
     3361 actctatacg gctgtacatg atcggcatgt tcctcagcag taccacaccc gggcaatcgg
     3421 gcgacttcgt gaaagcgtgg tacttgcggg acagaggatt accgctggct gcgtcgctct
     3481 tcagcgtgct ggtggaccgg ctgctcgact ttctggtcat ggcgttgctg agcctggtgg
     3541 ggctggttgc attcgtccat ctgtttccac agcaggctcg aggagcgatc gaagttgcta
     3601 cggtcggctt cgcggtcgcg attgcgctcg tgacgccggc actgatggat cggcgttcgc
     3661 gcaactggct catgagtcgt gccgtccggc tggtgcccgc caaggttggc ggcaggatcg
     3721 agcgctggcg gaatcaattc gccgcactcg atatccggcc ggggctcgtg gccggcctat
     3781 tcgccgggac cttgtgcgcg actaccgtga cgatggtgcg tctctggctt ctctttcggg
     3841 cgatggaggc agccatcccg ctggccgcga tggtaacgtc gatggcgttg atttccaacc
     3901 tgcagtccct gccgatcagc ttctcgggcg ttggcgtgcg agatgcggtt ctgattccgg
     3961 tgctggcata ctacggctac ggagccgaga aggccctggc cctgtctgcg ctggtccttc
     4021 tcctcaacat cgagaacatc ctgctggggt atctggtatc gctgcgccat cccctggggc
     4081 cgggaacctc gaccaggccg ccaactcgct tcccgcccgt gactgcctga ctcaagccgc
     4141 tgatgccgag gcgggagcga cgcatcggtc ggcgacctcg atcagcacca gagctccgcg
     4201 gtcggcaatc acgcacccga ggccgagcag ccggcgttcc tcttccgcgc caagcagcac
     4261 ctcgcgtcgg tcgaggagaa tccactgcac gtgatagcga gcgatcaccg cgtgccgttc
     4321 ttggtcggtg gcgtttccga agaactggtc ctgcgccttc cggcgttccg cttggtcggg
     4381 gatccagtac accggatgcc gccacgccac gacacggcct ccactgagcg cgggcaccag
     4441 gtaactcgtg cgggagtcgg caagaaccac gtcgcccggg cgcaccgggc ccaggatggc
     4501 tttggcctcg ggccacacct gccggtcaac tcgaatgatg cggggaaggg gcggcttgtt
     4561 ttgggcaaag agagcaagcg cggccacgac agaaccggaa ggcaccagcc aggaccggcc
     4621 cttcgcaacc cgtgactccc acccgaccgc tgcgatcgcg agcgcaatct gaatgaagac
     4681 cacgatgtac gcgatcgtgc gcccaagccc gtaacgaccg gtgatgccac cgattgcgta
     4741 gatgaccgcc agcaacgcca ccaggagcac cagcgggtcg cgtcgatcgt ctcggagacg
     4801 ccatagcagg accggcaacg caagaatggc gggccagatc tggcccacaa ctccctgata
     4861 gaagacaccg ctccagctgt ggaattcggg tggctggtcg gcaaacagct tgagcaccgg
     4921 gaagtagggc cacgtgaggg cgagagccac gcctgcagac accgctccga aaagcaatcc
     4981 ggctgtgatc cagaaacgtc gagctacccg ggccaagaac aaggctgcca gcccggcgaa
     5041 cagcaccaaa ccggtgggtg ggtgggtaag catgcacacg gcgacgagca gcgagattgc
     5101 cgcgaagagg ccagtcctcc cggagtcgag cgcgtccatc aggacggaga ggcagaggaa
     5161 catcgcagcc gcagcaaagg tcgaaggata gggtgcgttg tagcccagca tcccgatgtg
     5221 gaagaatccg ctccacatcc agacgtcctt gccccacagg aagatgatga aaagaagggc
     5281 atagggcgca gcggcgtcgc cgtgaggaag gcgcagcagg aaacgtcgaa tggctaggac
     5341 cagcaggacc atattgacca gtcctgcgat ggccagcatc gtcgtcggcg ctgcgccggt
     5401 cactcgcgca gccaggccga cggcaaggtg gtatggcgag gcgtaggcgt gcggagcatc
     5461 gactgcgagc agcggatgtc tgggcgccag cgggcgcacc gagagctccc gtaccacagc
     5521 cgcatgctcc cagaagtcgc tggagaagta gccgcgccac gcgtagaggg cgcagagccc
     5581 cagggcgccg acggctacag ccaggaaccg aagatgatcg cgccggcgca ggccggcagc
     5641 gtctacgtcg gggacgtgcg agtcgggacg cggatcgcga tcagctgcca cgccagggca
     5701 ctccatccga aggacagcgg taccagaagc gttcgaacca tccaccactt gtggatcgtg
     5761 ctgtgggtct ggaacgccag gatccaggcg aggacgatcg caccgccggc ggcgaagccg
     5821 agaagatcgc tggttgccca ccgaacgcgc cgccgcactg caatgaagcc ggcgatcaac
     5881 caggcaagcg cagaggcagc atataagacg atggcgccgg tatggctccc ataggtcagc
     5941 acgtatccct gactcagcac cgcttcgact gccttggcgc tggagacgag cagcgggtcc
     6001 ttgggtgacg accatgtctc accgaagtgc ttgaggctgg cacccggtga gggattgatg
     6061 taccggtgca ggtattcagc aaacgtgcgc acggcctccg ggccgaaaat cacgactgcc
     6121 aacgcctgct tgatggcgac ggtcaagagg gcgccaagcg caaaggcaag caaagccgcc
     6181 agagcaagac gccgtgccct caccggatca ttctcgggct caggtctgag ccgctcgacc
     6241 acgtatacgg tcggcacgag cagcccggct gcagtcggta acaagccggt caggaattca
     6301 aggtaggcga ccccagctcc gtaaaccgcg cagaggggaa cgagcatcga catgcttccc
     6361 gcccgtttgc gcaaggccag gaatccaaca atgccaagga tgacgaatgc gtctcccggt
     6421 ccgtgcgtca gtgactgacc gaagtaggga accccccaga agagcgcacc ggtcagcgcg
     6481 atgctggccg acaccgccag gagcaggcgt tggtggagtc cggtggcaat gaccagcagg
     6541 gccagagcgg catacacggt cagcttgagg accgtgcgaa cgttgcccag atccattacc
     6601 cagagcagcg ctgcgctgat ggggttatag ccgtgccagt agcgggtgta ccgatacacc
     6661 aagtaggagg cggtgtttgg cccttcggtt accagcgtgc gcagcgtcgg gcagcgatcc
     6721 gtttcccctc ggttccgatt gtacatcagc ggccctacgg cggcggccca ccgatggtcg
     6781 tccgcgttgg agatcatttg aagaactgaa caatcgtgat actgattgaa g
//
LOCUS       DASRJQ010000405         2256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2489858, whole genome shotgun sequence.
ACCESSION   DASRJQ010000405 DASRJQ010000000
VERSION     DASRJQ010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2489858"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="VFB89_09120"
     CDS             <1..779
                     /locus_tag="VFB89_09120"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HZM27506.1"
                     /translation="LKYRFQYVDKIPRPWRVAAMAFGTSVHAAVEWFHRERMEGSTPT
                     LEAMLQVFEADWYAQNTEPLVFNEKESQDSLRLKAAAMLEAYLAASHGPAPAAVEQFF
                     ELDLADPETGEVFDLRLRGVIDLIEEGDVLVDLKTAGRMLEAGGLERHLQLSTYALAL
                     YLRTGVIPKLRLDMLLKTAKPRLERLETSRSREDLAWVAQLIKEVASAIETEHFFPNP
                     SWRCSECEYFAHCQAWRGWTPTQGAIPAAAGGEGAGAAVA"
     gene            961..1383
                     /gene="radC"
                     /locus_tag="VFB89_09125"
     CDS             961..1383
                     /gene="radC"
                     /locus_tag="VFB89_09125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HZM27507.1"
                     /translation="MYVCELTRRRYRGKKPAEIRGPDDVVRVLGPKIRREQREHFMVL
                     LLNARHEVVAKETVSIGSLNASIVHPREVFKPAVVASAATVVLVHNHPSGDPEPSEED
                     LAITRRLVEAGDLLGIQVLDHVIVAERGVVSLRARQLL"
     gene            1393..1791
                     /locus_tag="VFB89_09130"
     CDS             1393..1791
                     /locus_tag="VFB89_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27508.1"
                     /translation="MGVSVDLIYEDLRCKSADDARKAAATIAADEWIHPYHLQVVPAT
                     RLHPGPGITPCLEVTHFKGDHWHDERARRVWLAIAPHLANGSTLEFEGEDSTRWRIRW
                     EGSRVFEEHVTEVIWAVNEEITAPEAEKQS"
     gene            1788..>2256
                     /locus_tag="VFB89_09135"
     CDS             1788..>2256
                     /locus_tag="VFB89_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27509.1"
                     /translation="MTLIVHRGGWQATKADLAAVPVPEPTASYHPVPYARFIEEVELH
                     VPRFGLTVQSSQFALAREGSQMFGVLTCVNGRPQADYALAVGLRNSYDRSLSVGLTFG
                     SRVFCCDNLAFSGEVTMHRKHTVHVFRDLPDLIYRMLSQVSSMRERTDREIGAI"
BASE COUNT          390 a          780 c          705 g          381 t
ORIGIN      
        1 ccctcaagta ccgcttccag tacgtggaca agatcccccg gccctggcgg gttgccgcca
       61 tggccttcgg cacgtccgtt cacgccgcgg tcgaatggtt ccaccgggaa cgcatggagg
      121 gctccacgcc gacgctcgaa gcgatgctcc aagttttcga ggccgactgg tacgcccaga
      181 ataccgagcc gctggtcttc aatgagaagg agtcgcaaga ctccctcagg ttgaaggcgg
      241 cggccatgct cgaggcctac ctcgccgcga gccatggtcc ggcgccggcc gcggtcgagc
      301 agttcttcga actcgacctg gcggaccccg aaaccggcga agtgttcgac ctccggctcc
      361 ggggggtcat cgatctgatc gaagagggcg acgtgctcgt cgacctcaag accgccggcc
      421 ggatgctcga agccggtggc ctcgagcggc acctgcagct ctcgacctac gcgctggcgc
      481 tctatctccg aaccggcgtc atcccgaagc tccgcctcga catgctcctc aagacggcga
      541 agccaaggct cgagcggctc gaaacgagcc gttcccgcga agacctcgcc tgggtcgcgc
      601 agctaatcaa ggaagtcgcc agcgcaatcg agaccgaaca cttctttccc aatcccagct
      661 ggcgctgcag cgagtgcgag tatttcgccc actgccaagc atggcggggc tggactccga
      721 cgcagggtgc gatacctgcg gcggctggcg gggaaggtgc cggggcggcg gtggcgtgag
      781 ggctctttag gagccggttc atgctaacgg gggccatggc tggcgcctga atccccgatc
      841 accactcgcc aaaccgcacc ttctcttgcc gaaactcaac gcacctttca cctccatccg
      901 gggctcctgg ctttccaagc aggggcccct ttttctaccc gctcccgtgg gaaaagcctc
      961 atgtacgtct gcgaactcac taggcgccgc tatcgcggga agaagcctgc ggagatccgc
     1021 ggtcccgatg acgtcgtcag ggtgctcggc cccaaaattc ggcgcgaaca gcgcgagcac
     1081 ttcatggtgc tgctcctgaa cgcccgccac gaggtcgtgg ccaaggagac cgtctcgatc
     1141 gggtccttga acgcctcgat cgtccacccg cgcgaggtct tcaagccggc ggtagtcgcc
     1201 tcggccgcca cggtcgtgct ggtccacaac cacccatccg gcgatcccga gccctcggaa
     1261 gaggaccttg cgatcactag gcggctcgtc gaagccggcg atttgctggg gatccaggtt
     1321 ctcgatcacg tcatcgtcgc cgagcgcggg gtcgtctccc tgcgtgcccg gcagctgctc
     1381 taggaggcgc gcatgggagt ctcggtcgat ctcatctacg aggatctgcg ctgcaagagc
     1441 gcggacgacg ctcggaaggc cgcggcgact atcgcggctg acgagtggat tcacccctac
     1501 cacctgcagg tggtgccggc cacccgcctc caccccggcc cgggtatcac cccgtgcctc
     1561 gaggtcaccc acttcaaggg cgaccactgg cacgacgagc gggcccggcg ggtgtggctc
     1621 gccattgcgc cgcacctcgc gaacggctcc actctcgagt ttgagggtga agactccacg
     1681 cgctggcgca tccgctggga gggcagccgc gtcttcgaag agcacgtcac cgaagtcatc
     1741 tgggccgtca acgaagagat caccgccccc gaagcggaga agcagtcatg accctcatcg
     1801 tccaccgcgg cggttggcag gccaccaagg ccgacctcgc cgccgtccct gtccccgaac
     1861 cgacagcgag ctaccacccc gtcccgtacg ctcgattcat cgaagaagtg gaactccacg
     1921 ttccccgctt cgggctcacg gttcagtcct cacagtttgc gctcgcgcgg gaaggaagcc
     1981 agatgttcgg cgtcctgacc tgcgtgaatg gccggccgca agcggactac gccctcgcag
     2041 tcggcctgcg gaacagctat gaccgctcgc tgtcggtcgg gctgacgttc gggagccggg
     2101 tgttctgctg cgacaacctc gccttctcgg gcgaggtgac gatgcaccgg aagcacacgg
     2161 tccacgtctt ccgggatctg ccagacctca tctaccggat gctctcgcag gtgtcttcga
     2221 tgcgcgagcg gacggatcgg gagatcgggg ccatta
//
LOCUS       DASRJQ010000406         2115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2052068, whole genome shotgun sequence.
ACCESSION   DASRJQ010000406 DASRJQ010000000
VERSION     DASRJQ010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2052068"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1340)
                     /locus_tag="VFB89_09140"
     CDS             complement(156..1340)
                     /locus_tag="VFB89_09140"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZM27510.1"
                     /translation="MSRPLSIRLGWIAALAFASGFPFGLINEALPVYLRTQGAGLVDI
                     GRLAAITAPWSLKFLWAPLVDRLGTRRQWIAGCLAILAVLTLVLAHSDPARLGPAFWL
                     LAIAIVTLSATQDIAIDAYTIEITSTQELGVANSVRIAAYRVAMFTAGGLLIWLAGRA
                     DWSVSFTAGTTVLALLTVAALLMPAPEGTGDRPHALWEPLRALGVRPGIWAVVTFALL
                     FKLDIAALEPMTRPFWVDRGFSLTEIGAVLTTGRIIATLAGAALGGVLTSWWGIFRGL
                     WILGLVQAFSSLGYWTAATAVPSKSLILSAALFENFAAGMGTAAYLAFLMSICEKRYA
                     ATQFALLSALLAITRAVAGEFSGGLAERMGYGSYFLLTFALAFPAYALLPAIKRAVLE
                     KA"
     gene            complement(1337..>2115)
                     /locus_tag="VFB89_09145"
     CDS             complement(1337..>2115)
                     /locus_tag="VFB89_09145"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HZM27511.1"
                     /translation="FEYAREHPELRGMGTTATVAGVLGDGLWLAQIGDSRAYLARGGE
                     VIQLTKDQSLMQRLVDAGELTQDEADHSERRNIILQALGPDPRVKVDLTHQKLRRGDT
                     LVICSDGLSSQVRREEIGELIETSNGLADLCDALIDLANGRGGPDNITVVVARFEGEG
                     LPDFQGAGGVGYHVYRLPDSATPTAERAVPAGPRQEVAPESSAERPVVESSAPAEIPA
                     QEEPEPPPEGFEARGATGRPLLLALGLAVLAALLFYIAIR"
BASE COUNT          405 a          695 c          679 g          336 t
ORIGIN      
        1 cctggttctg ctattcgccc atccccgact ggtggcagac attcccggct cggctcctgt
       61 cttgctcgcc tggcatcggc agagactgat gcaggaggcg ggagcgaggt ggatcgtcgg
      121 tagaacttcg tgatcagtgg caaagcgttc accgttcacg ctttttctag aaccgctcgc
      181 ttaatcgccg gcaacaatgc gtacgccgga aacgccaacg caaaggtcag caggaaatac
      241 gatccatagc ccattcgctc ggccagcccg cccgaaaatt ctcccgcaac agcacgggtg
      301 atcgcgagca gcgcggacag aagcgcgaac tgcgttgcgg cgtagcgctt ctcgcagatc
      361 gacatcagaa acgcgaggta agctgccgtt cccattccgg ccgcgaagtt ctcgaacagg
      421 gccgcgctca ggatgagcga cttcgagggc actgccgttg cggctgtcca atagccgagg
      481 ctcgagaatg cctggactag gccgaggatc cagagtcccc ggaagattcc ccaccaggag
      541 gtgagcacgc cgcccagagc cgcgccggcc agcgtcgcaa tgatgcgacc ggtggtgagc
      601 acagcgccga tctcggtgag cgaaaagccc cgatccaccc agaacggccg ggtcatcggc
      661 tcgagtgcag caatgtcgag cttgaacagc agggcaaacg tcacgaccgc ccaaatcccc
      721 ggacggacgc ccagcgcccg aagcggttcc cagagtgcgt gaggccggtc accagtgccc
      781 tcgggggcgg gcatcaagag cgctgcgacg gtgagcaggg cgagcacggt cgtcccggcc
      841 gtgaagctca cgctccagtc ggcccggcca gccagccaga tcaggagtcc cccagcggta
      901 aacatcgcaa cccgatacgc ggcgatccgc accgagttcg cgacgcccag ttcctgggtg
      961 ctggtgatct cgatcgtgta ggcgtcgatc gcgatgtctt gcgtcgcaga gagcgtgacg
     1021 atcgcaatcg cgagaagcca gaaggcaggg ccaagtcggg cagggtcgga atgcgcgagc
     1081 accagggtca gcaccgcgag tatcgcaagg cacccggcga tccattggcg gcgggtcccc
     1141 aggcgatcca ccagcggcgc ccagagaaac ttgaggctcc agggcgcagt aatggcggcc
     1201 agacggccga tgtccaccag cccggccccc tgcgtgcgca ggtagaccgg gagggcttcg
     1261 ttgatcaatc cgaaagggaa accggaggcg aaggcgagcg ctgcgatcca gccgaggcga
     1321 atggagagcg ggcggctcac cggatggcga tgtaaaacag aagcgctgca agcactgcga
     1381 gcccgagcgc cagcagcaga ggccggccgg ttgcgccgcg ggcctcgaat ccttccggcg
     1441 gcggttccgg ttcttcctga gcagggatct cagccggagc cgaactctct acgaccggtc
     1501 gctctgcgga gctctcgggt gcgacctcct gtcgtggccc cgcgggtacc gcgcgctctg
     1561 cggtgggcgt ggcggagtcc ggcagccgat agacgtgata gccgacgccg cccgctccct
     1621 gaaaatccgg cagcccctcg ccttcgaatc gggcgaccac cacagtgatg ttgtccggac
     1681 cgcctcgacc gttggcgagg tcgatcagcg cgtcacagag atccgccagc ccattcgacg
     1741 tttcgatcag ctcgccgatc tcctcccgcc gaacctggct cgagaggccg tccgaacaga
     1801 tgaccagggt gtctcccctg cgcagctttt gatgggtgag atccaccttc acgcgcggat
     1861 cgggtccgag cgcctggaga atgatgttgc gtcgctcgct gtgatcagct tcatcctggg
     1921 tcagctcacc tgcatccacc aggcgttgca tcagcgactg gtccttggtg agctgaatga
     1981 cttctccacc ccgggccaga taggcacggc tgtcgccgat ttgcgccagc cagagaccgt
     2041 ctcccaagac tccggccact gtggccgtcg tgcccatgcc tctcaactcg ggatgttccc
     2101 gcgcgtactc gaata
//
LOCUS       DASRJQ010000407         2587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3090950, whole genome shotgun sequence.
ACCESSION   DASRJQ010000407 DASRJQ010000000
VERSION     DASRJQ010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3090950"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VFB89_09150"
     CDS             <1..220
                     /locus_tag="VFB89_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27512.1"
                     /translation="TRKVAVALQPRLESYRGYDYEAVFLLAALRAGFRVVSIEVPTIY
                     TSAPSHFRHWGDGWKVARVFARYFRGAA"
     gene            217..966
                     /gene="surE"
                     /locus_tag="VFB89_09155"
     CDS             217..966
                     /gene="surE"
                     /locus_tag="VFB89_09155"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001489.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HZM27513.1"
                     /translation="MKILLSNDDGILARGLAVLADACNELGQVTVIAPDREQSATSHS
                     LTLHRPLRATRRSDGAYQVDGTPTDCVLLGLDALLPAKPDFVLSGVNHGPNMGEDVLY
                     SGTVAAAMEGLAAGIPSVAISYGSFDLEHLESHSAGLKRLIGRIVTVDQFPRDTLLNI
                     NLPPIPGDQVRGVKVTSLGSRVFHEEIARMKDPWGREIYWIGGGHVTWSGGEESDFHA
                     VREGFVSVTPLHVDMTNYNLLEVVRGWSLGT"
     gene            948..1616
                     /locus_tag="VFB89_09160"
     CDS             948..1616
                     /locus_tag="VFB89_09160"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003222172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HZM27514.1"
                     /translation="MESGNIGASADTFGGYRARLVERLRDQGIQDLAVLRAFAETPRH
                     LFVPTAVRHRAYEDGALPIGAGQTISQPFTQARYLEALRLTGAERVLEIGTGSGYQTA
                     LLANLVSQVFSVERVGELAEAARRALREAAVRNASVMLGDGTLGWSDYAPYDAILVAA
                     GGPEIPTPLIDQLTLGGRLLIPLGDKDAQTLTLVERRTDGLRQTPLGAALFVPLLGEH
                     GFDA"
     gene            1609..2244
                     /locus_tag="VFB89_09165"
     CDS             1609..2244
                     /locus_tag="VFB89_09165"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HZM27515.1"
                     /translation="MLESLIQGLLHGLRDLGYPGIVALMALESSVFPVPSELVMSPAG
                     YWVAKGEMNAVLVVASGVVGSIIGALANYGVAWSLGRAFVRRFGKYVLLSERSLERSE
                     RFFTAHGEISTLIGRMVPVVRHLISIPAGIARMPLPRFIAYTGAGALIWCSILTWVGW
                     FLGKHESSLPSADEIQRYTGKVLLVLIPTLVVVVVAYVWWYRRRTARRAGE"
     gene            2252..2524
                     /locus_tag="VFB89_09170"
     CDS             2252..2524
                     /locus_tag="VFB89_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HZM27516.1"
                     /translation="MVQARYVVRGRVQGVGFRWFVLHQASALDLGGYVRNLPDGSVEV
                     LASGAAAGLTRLEQALHRGPSSARVENVEKSDVPHDVKLPKPFDVR"
BASE COUNT          429 a          785 c          903 g          470 t
ORIGIN      
        1 cacgcggaaa gtcgcggttg cgttacagcc gcgcctcgag tcctaccgcg gctacgatta
       61 cgaggcggta ttccttctgg cggccttgcg cgccggattc cgggttgtgt cgatcgaagt
      121 tccgacgatc tatacgagtg cgcccagcca ttttcggcat tggggcgatg gatggaaggt
      181 ggcgcgcgtg ttcgcgcgct atttccgtgg agcggcatga agatcctgct cagcaacgac
      241 gatggcattc ttgcccgcgg gctcgcggtc ctcgccgacg cctgcaacga gctggggcag
      301 gtgaccgtga tcgccccgga ccgggagcaa agcgcgacct cgcattcgct cacgctgcac
      361 cggccgttgc gggccacccg tcgcagtgac ggcgcctacc aggtggacgg cacgccaacc
      421 gattgcgtgc tgctgggact cgacgcgctg ctgccggcaa agccggattt cgtgttgtcc
      481 ggagtgaacc atggccccaa catgggcgag gatgtgctgt attcggggac cgtcgcggcc
      541 gccatggagg gactggcggc ggggattccg agtgtcgcga tttcctacgg cagtttcgac
      601 ctcgagcacc tcgaaagtca cagcgccggt ctgaaacggc tcatcggtcg gatcgtgacc
      661 gtcgaccagt ttccccggga cacgctgctc aacatcaacc tgccgccgat ccccggcgac
      721 caagtccgcg gcgtgaaagt gaccagcctc ggcagccgcg tgtttcacga ggaaatcgcc
      781 cgcatgaaag atccctgggg gcgcgagatc tactggatcg gcggcggtca tgtcacctgg
      841 tccgggggcg aggaatcgga cttccacgcc gtgcgcgaag ggttcgtgtc cgtcacaccg
      901 ctgcacgtcg acatgaccaa ttacaacctg ctggaagtcg tacgcgggtg gagtctggga
      961 acataggcgc gagcgccgat acgttcggcg gctaccgcgc ccgattggtg gagcggctgc
     1021 gagaccaggg gatccaggat ctggccgtgc tgcgggcatt cgcggaaacg ccacgccacc
     1081 tcttcgttcc cacggcagtc cggcaccggg cgtacgagga cggcgcgctg ccgatcgggg
     1141 cagggcagac gatctcccag ccgtttacgc aagcccgcta cctcgaggcg ctgcggctca
     1201 cgggagccga gcgcgtgctc gagatcggca ccggctccgg ctaccagacg gccctgctcg
     1261 ccaacctggt ctctcaggtc ttcagcgtcg agcgcgtggg cgagctggcg gaggccgcgc
     1321 gccgcgccct gcgtgaggca gcggtgcgga atgcgtcggt catgctgggc gacggcacgt
     1381 tgggatggag cgactacgcg ccgtatgacg ccatcctcgt ggcggcgggc ggaccagaga
     1441 ttcccacgcc gctcatcgac cagctgaccc tgggcgggcg gctcctgatt cccctcggtg
     1501 acaaggatgc gcaaacgctg accctggtcg agcgacggac cgacggtctt cgccagacgc
     1561 cgctgggagc ggcactcttc gttcccctgc tgggagagca cggcttcgat gcttgaatcg
     1621 ctcattcagg gactgctgca cgggttgcgc gacctcggat atccgggaat cgtggcgttg
     1681 atggccttgg agtcatcggt atttccggtg cccagcgagc tggtcatgtc cccggcgggc
     1741 tactgggtgg cgaaaggcga gatgaacgcg gtgctggtgg tggcgagtgg tgtcgtgggg
     1801 agcatcatcg gcgcgctcgc gaactatggt gtcgcatggt cgctcgggcg cgccttcgtc
     1861 aggcgattcg ggaaatacgt cttgctcagc gagcgttcgc tggagcgctc ggagcggttt
     1921 ttcactgcgc acggagagat cagcaccctc atcgggcgca tggtacccgt agtgcgccac
     1981 ctgatctcga ttcctgcggg gatcgcccgg atgccgttgc cacggttcat cgcgtacacc
     2041 ggggcgggcg cgctcatctg gtgcagcata ctgacctggg tcgggtggtt ccttggcaag
     2101 catgaaagtt cgctgccgtc ggcggacgaa attcagcgct acacgggaaa agtgctgctc
     2161 gtgctgattc cgaccctggt ggtggtggtg gtggcgtatg tctggtggta ccgacgccgc
     2221 acggcacgcc gcgcagggga atgaggcgga agtggtgcaa gcacggtacg tcgtccgggg
     2281 acgggtccag ggcgtggggt ttcggtggtt cgtgctacac caggccagcg ccctggatct
     2341 cggggggtac gttcggaacc tgccggatgg ctccgtcgaa gtgctggcga gcggagccgc
     2401 ggccggtctg acgaggctgg agcaggcatt gcataggggg ccatcgagtg cgcgcgtgga
     2461 aaatgttgaa aagagcgacg ttccacatga tgtcaaacta cccaaaccct ttgatgtccg
     2521 atgattgaca acgaagctat caagcgagcc atacgcgacg tcccggactt tcccaagccg
     2581 ggcatca
//
LOCUS       DASRJQ010000408         3197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_654196, whole genome shotgun sequence.
ACCESSION   DASRJQ010000408 DASRJQ010000000
VERSION     DASRJQ010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654196"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2247)
                     /locus_tag="VFB89_09175"
     CDS             complement(<1..2247)
                     /locus_tag="VFB89_09175"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZM27517.1"
                     /translation="MGKGPSLLRSKFGRRLLALFVGCALGPIALLALLSERHMTRQLE
                     NQTRNRLVQANRALGRAIFERLLLLEATLASIPPGAVAELEAPSQPIEKPPVRPGNRP
                     VARSNRTQGSRLDGRIALGGIMVGGARGARKAPAPPAVPLKDSDANQTITAGLDLLAR
                     SRFTALEFVDEGGHRTQVFGRLDRMPTLEADDSAYVWAGETLLLAGDADETGRARIYM
                     LRRLGWGRGGGIRGTLVGEISPDFLWGTRDQTMPSPTSEMAVVDDAGRALFGVAPVPV
                     NASQRVLDLARQSASGSINTTIGGAAYLTTYRPIVIQGAFGAPNWTLVLSENQASMLG
                     PMTDFTDTFLFIVGSCTLAVLVLSVLQIRRSLLPLQELQAGTRRIALQDFEGRVALTS
                     RDEFAELGHSFNAMAAQLGRQFKALATAAEIDRTVLSATDASGIVDAVLTRLADVHPC
                     SAASVTLLPLDRSGSAVSWIQHYEGHRSREVIRVNLSQSELQRLAQAIDVLALHSESE
                     LTDLLRPLAAPGAASFIVLPLLAQGQLSGVLSLAVSATEPGSSEDLVQARRLADQVAV
                     ALSNAGMIEQVRALAYYDSLTGLLNRPSYKERLAGALANARRKHNQVAVFFLDLDNFG
                     RINDTLGHELGDQLLRQVAERLHCCCAEAAHERQGGPAGRGTAVARLGGDEFTVLLPA
                     MEDAEEAPALARRILSAFAAPIQLGVHEVFMTSSIGIALYPADGDDVEMLLAHADTAM
                     YEAKKQGGN"
     gene            complement(2363..3100)
                     /locus_tag="VFB89_09180"
     CDS             complement(2363..3100)
                     /locus_tag="VFB89_09180"
                     /inference="COORDINATES: protein motif:HMM:NF016367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(5-formylfuran-3-yl)methyl phosphate synthase"
                     /protein_id="HZM27518.1"
                     /translation="MSVRDPSEVAPALMGGAQIIDAKEPSNGALGPVTLACLQQIEEQ
                     VPPTVPLSVALGDFVSAEAAAEAILALPLRRRSASLYVKLAPSADRADGNRSVLDGAV
                     RAARRHRAAPAVIAVEYADRSRDPESSERVRSGAVLARVRGVLVDTAVKDGTSLLDWW
                     SEDRLMAWSRSAREAGLLVAFAGSLGVSEVARAMGFGCDVIGVRGAACVGGRAGRIDA
                     GLVRRLREVTDSIVPTPRTATVLSGCF"
BASE COUNT          586 a         1036 c         1027 g          548 t
ORIGIN      
        1 gtttccaccc tgctttttgg cctcgtacat cgccgtgtcg gcgtgagcga gcagcatttc
       61 gacatcgtcc ccgtctgcgg gatacagtgc gataccaatg ctggacgtca tgaaaacctc
      121 gtgcactccg agttgtatcg gcgcagcgaa ggccgacagg atgcgacgcg ccagcgctgg
      181 cgcctcctcg gcgtcttcca tcgccggcag cagcaccgtg aactcgtcgc ccccgagccg
      241 ggcaacggcg gtcccgcgcc cggctggccc cccctgtcgt tcgtgcgcgg cttccgcgca
      301 acagcagtgc aatcgctccg cgacctgtcg aaggagctgg tcgccgagct cgtggcccaa
      361 cgtgtcgttg atacgaccga agttgtccaa gtcgaggaag aacaccgcca cctgattgtg
      421 cttccggcgg gcattcgcca gggcgcccgc aagcctctcc ttgtacgaag ggcgattgag
      481 caacccggtg aggctgtcgt agtaggcgag ggctcttacc tgctcgatca tgccggcgtt
      541 cgacaatgcc acggccacct ggtccgccag gcgccgcgcc tggaccaggt cctcggagct
      601 gcccggctcg gtcgctgaca cggcgagaga taggacgccc gacaattggc cctgagcaag
      661 caagggaagc acgatgaacg atgcggcgcc tggcgctgcc aggggacgca ggagatctgt
      721 gagctcgctt tcgctgtgca gggcgagaac gtcgatcgcc tgcgccaacc gctggagctc
      781 gctctgactc agattcacgc ggatcacttc tctcgaccga tggccttcgt aatgctggat
      841 ccagctgacg gcgctgccgg atcgatcgag aggaagaagc gtcacgctgg cggcgctgca
      901 tggatggacg tccgcgagcc ttgtcagcac ggcatcgacg attcccgacg catcggtagc
      961 actcagcaca gtgcgatcga tctccgccgc cgtggccagg gccttgaatt ggcgcccaag
     1021 ttgggctgcc atggcattga atgagtggcc aagctcggcg aattcgtcgc ggctggtcaa
     1081 cgccactcga ccctcgaagt cctgcaacgc aattcttcgg gtcccggctt gcaattcctg
     1141 cagagggagc aggctgcggc gaatttggag gacgctcaga acgagtacgg ccagggtgca
     1201 actgcccacg atgaacagga acgtatcggt gaagtcagtc atcggcccca gcatggatgc
     1261 ctgattctca ctgagcacca gcgtccaatt gggcgccccg aaggcaccct gaatgacgat
     1321 aggccgatag gtggtgagat atgcggcgcc cccaatggtg gtgttgatgc tgccggacgc
     1381 cgactgacgc gccagatcga gcacgcgttg ggaggcattg accggcaccg gcgcgacgcc
     1441 gaacagggct ctcccggcat cgtctaccac agccatttcg ctggtgggcg aaggcatggt
     1501 ctgatcccgg gtgccccaca gaaagtccgg gctgatttct ccgaccagcg tgcctcggat
     1561 accaccgccc cgcccccatc cgagccgccg gagcatgtag atccgggcgc gcccggtttc
     1621 gtccgcatcg ccggcaagaa gcagcgtctc gcccgcccac acgtaggcgc tgtcgtccgc
     1681 ctcgagcgtt ggcatccggt cgagccggcc gaacacctgc gtgcggtgcc caccttcgtc
     1741 cacgaactcg agtgcagtga atcggctgcg ggccagaagg tcgagtccgg cagtgatggt
     1801 ctgattcgcg tcggaatctt tgagcggcac cgccggcgga gccggcgcct tccttgcccc
     1861 ccttgccccg cccaccatga tgccacccag cgcaatccgt ccgtccagac gggatccctg
     1921 ggttcgattg ctgcgggcaa ccggccgatt ccctgggcgg accggcggct tttcgatcgg
     1981 ttgggatggc gcttctagct cggcgaccgc gccgggcgga atgctggcga gggtggcttc
     2041 gagcaacagt aggcgttcga aaatcgcccg acccaaggcc cgattggcct ggaccagtcg
     2101 attacgagtt tggttctcca gttggcgggt catgtgccgc tcggacaaaa gtgcgagcag
     2161 cgcaatcggt cccagggcgc acccgacgaa gagcgccaga aggcgacggc cgaacttgct
     2221 gcgaagcagg ctggggcctt tgcccatgag acggatgccc ggtgggagtg gggagcatcc
     2281 ggcgcgatcg ggcaaatgac gtgccccttt gggcctcggc aagtaagcgt aagtgcatgt
     2341 tttacttaga aatggagatg agctagaagc acccggaaag tactgttgcg gttcgcggtg
     2401 tcggcacgat tgagtcggtc acctcacgga gccggcggac cagcccagcg tcaatgcggc
     2461 cggcccttcc tcccacgcag gcagcgcccc gcactccgat cacatcgcag ccgaagccca
     2521 tggcgcgagc aacctccgaa acgcccagac tacctgcaaa ggccaccagc agtccggctt
     2581 ccctcgcgct cctcgaccag gccatcagcc gatcctcgga ccaccagtcc agcagggatg
     2641 ttccgtcctt gaccgcggta tcgaccagga cgcctcggac gcgtgcgagg acagcaccgg
     2701 agcgaacgcg ctcggagctt tccgggtcgc gagatcggtc ggcgtattcg accgctatga
     2761 ccgcgggggc ggcgcgatgt cgccgagctg cacggacggc accatccaag acggatctgt
     2821 tgccatcggc acgatcggcg gagggcgcaa gcttgacgta cagcgacgcg ctacgtcgcc
     2881 ggagcggaag cgcgagaatt gcctcggccg ctgcttcagc cgagacgaag tcgcccaggg
     2941 ccacgctcag gggaacagtt gggggaacct gttcttcaat ctgttggaga cacgcgagcg
     3001 tgaccggccc gagcgctcca ttcgagggct ctttcgcgtc gataatctgc gccccgccca
     3061 tcaacgccgg ggccacctcg gaagggtctc gaacgctcac caatagcgac aaatgagagc
     3121 ttcccttgtg agttgctaag gcaatgatac tttagcgctt agaagtttac gtcaggcgat
     3181 tcaaccgggg gaggccg
//
LOCUS       DASRJQ010000409         6675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_333594, whole genome shotgun sequence.
ACCESSION   DASRJQ010000409 DASRJQ010000000
VERSION     DASRJQ010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6675
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333594"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="VFB89_09185"
     CDS             complement(<1..853)
                     /locus_tag="VFB89_09185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZM27519.1"
                     /translation="MGRAAPEGKHLKDRVDGGIAVVEAAPAEEPESQEPGRVSPPSAE
                     QLQTWLLICALGAGVVCRAVYYLSGRSLWIDEARVALNVGTRGYAALLRPLDYDQAAS
                     PLFLWGEKLMSQLFGMNERALWLLPFVAGVLALILFVPMARRLLPGWLGVIAIASFCI
                     APTLVHFSANAKPYIGDLTFALAILLGAFAWIERPESRFARWLPLVGVIAPWVSTPAI
                     FTLVPAAGAMVLAAPERRTRAIFIGVLWLVSFVVAYALVYAPGAHNEYLRRFWWYSFL
                     RPWEPGLV"
     gene            1108..3237
                     /locus_tag="VFB89_09190"
     CDS             1108..3237
                     /locus_tag="VFB89_09190"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZM27520.1"
                     /translation="MDPDRFRRLPPGAAAVSLPASDRSAAAPSRRVPLPDPPRAAQLE
                     WVDLRNGDRREDEFHWLQKRDHPAVLQYLEAENDYATLAMQHTEALQERLYLELLGRT
                     DETDESVPDRIGPWLYYQRFEAGRQYPVLCRKPVCEGAPEQVLLNLNQLAPRPHLRLG
                     ASEISPEHGYLAFALDFSGDEAHTLFIRDLTAGCLLSENICGTANTLAWADERTLFYT
                     VVDKVSRPWAVFRHRLGTDATTDVMVYAEVDDAFSVEVSRARGGEWILIESASGGSTE
                     IRSIPAQRPFEAPRVLLPREPGVEYAVAPLGDRLFILTNEAAENFRLVEASLANPNRT
                     EWGEILPHSASVKLDAIDVFRDHLVVWEREAGLPWVRVLDLATGAQHRVELPEPANAI
                     SRSSNPELDTTTVRFSCTSLVSPLAVMDYDMATRTLTLRKQATVHGYDRTRYRADRLT
                     TEAPDGSPVPISLIYRWPFPLDGSRPGLLLGYGAYGTAFEAGFQSHFLSLLDRGFVVA
                     IAHVRGGDELGRNWYQQGKLERKRNTFTDFIAAAEYLIAEGYTSAHRLAINGASAGGM
                     LMGAVTNLRPELFRVVVAEVPFLDVVNTMLDPSLPLTITEYDEWGDPRDAATYAYMRS
                     YSPYDNIQQKAYPHMLVTGGLNDQRVPYWEPAKWTARLRARKTDNNRLLLRTNMGAGH
                     AGASGRYDVIREVAFKYAFILDVLGVS"
     gene            complement(3239..5461)
                     /locus_tag="VFB89_09195"
     CDS             complement(3239..5461)
                     /locus_tag="VFB89_09195"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HZM27521.1"
                     /translation="MPAEDRHRATRVLYALAVFLSAGLLFVVEPMVGKMAVPMLGGTP
                     AVWTTCLLFFQVALLAGYLYAHLIQAQLALRPQVWLHLGLLAVALATLPVALPANDLP
                     QSGQSPIGWLLVALLRTVGAPFILLAATAPLLQRWFSRLDHPDASDPYFLYAASNLGS
                     FTGLLAYPFLIEPWLGLSSQRTAWTVGYIALIVAIGLVGLWAVRQSNPEPSKKAATKA
                     APPAPSEPKAKPWRWLLLAAVPSSLLLGVTTHLSTDIAAVPLLWVLPLALYLLSFVAT
                     FARHPWISPRIAQHWLPLLFLQLVAAVMGEASLRTSLALNYGMLFVAGLLCHGQLARL
                     RPPAERLTAFYLWVALGGALGGAFNVLVAPVVFSNVTEYPLAIAATAALATWTPILTG
                     AAGVVTIGLSMVALAVVLGHTAVALEVRRVLPLNLRSLWFLNAAAIAAMFGALLLWRR
                     RFHLMLGLGLPLAVGLHWAVVRRHPGLIYLARDFFGVYRVQYTRGGGFRVLYHGTTIH
                     GLQSRFPPWRRLPTSYYHPEGPLGYIMLGVAPPKPGRRVGVVGLGAGTTAAYAGPGES
                     WTFYEIDPLVERIARDTTLFTFLVDARSPPRIVMGDARISLAHDSTARYDVMILDAFS
                     SDAPPIHLLTREAVRLYFQRLAPGGLLAIHLSNRYINFPPVVAALAADAGLVGRVWGQ
                     EPPLAQIYRGAFGSTWAVLARREEDLGRITRDPRWERFEAVPGALWTDDYSNIYRRIR
                     "
     gene            complement(5508..6278)
                     /locus_tag="VFB89_09200"
     CDS             complement(5508..6278)
                     /locus_tag="VFB89_09200"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZM27522.1"
                     /translation="MQPEHSGRPLALFRLTPPYNRFEELPDLDASEADLPLGSVLVIE
                     LTSSNDWPAVEALAPRLRARFPAAPLALRVRSGVGIDPAEAARIAAPFHIRAVLGDDA
                     PPRETLQTVLTDPASLTHELEDWLAVRGVDPPDPLRNLILDIFRQAPSHPRLHGLLQS
                     LGRPERTVRNWFQRAGLPGPGAWLAAAHAVYAALRLQRERQTSLLTIAVECGYSDHSS
                     LSRQAVRLFGARPGEIRQTLGWEGLLDRWLGRAGVGTA"
BASE COUNT         1310 a         2168 c         2142 g         1055 t
ORIGIN      
        1 tcaccagccc tggctcccac ggcctcagaa agctgtacca ccagaatcgc ctgaggtatt
       61 cattgtgcgc acccggagcg tagactagcg cataagccac cacaaaggag accagccaaa
      121 gcactccgat gaaaatggcc ctggtccgcc gctccggcgc cgccagcacc atggcgccgg
      181 ctgccggcac cagggtgaag atggcggggg tcgagaccca gggagcgatc acgccgacaa
      241 ggggcagcca acgggcaaag cgcgactccg gccgctcgat ccaggcaaag gcacccagca
      301 gaatggccag agcgaaggtg aggtctccga tgtacggctt ggcgttggcg gagaagtgaa
      361 ccagcgtggg agcgatgcag aacgacgcaa tcgcaatcac acccaaccag cccggtagca
      421 atcgccgcgc cattggaacg aacagaataa gagcaaggac gccagctacg aacggaagca
      481 accataatgc gcgctcgttc atgccaaaca gctggctcat cagtttctcg ccccacagaa
      541 acaggggcga cgctgcctgg tcgtaatcga gcggtcgcag caatgcagca tacccgcgcg
      601 tgccgacatt gagcgccacg cgcgcttcgt cgatccagag cgatcgacca ctcaggtagt
      661 agaccgcgcg acaaacgacg ccagcaccca gagcgcagat gagtagccac gtttgcagct
      721 gctccgctga cggtggcgaa actcgacctg gctcttgcga ttcgggttct tcggctggcg
      781 ccgcctccac cacagcgatc ccgccgtcga ctcggtcctt cagatgcttt ccttcggggg
      841 ctgctcgccc catctcataa cgtcgtattc tgcgctacta cctgcgccga taccgacgac
      901 gcttctgtag ttagctcgtc ttttttcatc gccgcgctcc gctcccaagc caagcccctg
      961 cccggaaaca ggttatcgaa tcagccgatc gtgtcgccca cccgtacctt ggctccgcat
     1021 cgcaactctt ttcctaccaa ggcgttaaat cggcttgact tggctcaggc tgcccgggta
     1081 attgcgctga accgctccga ggccactatg gaccccgatc gttttcgccg cctcccgcct
     1141 ggcgcagctg ccgtcagtct cccggcgagc gaccgttctg ctgccgcccc cagccgccga
     1201 gttccgctgc ccgacccacc ccgcgcggct cagctcgagt gggtcgatct ccggaacggc
     1261 gaccggcgtg aagacgagtt tcactggctc cagaagcgtg atcatccagc cgttctccag
     1321 tacctcgaag ccgaaaacga ctacgccacg ctggccatgc agcacaccga ggccctccag
     1381 gaacgcctct atctcgagtt gctcgggcgc accgacgaaa ccgacgaatc ggtacccgac
     1441 cgaatcggcc cctggctcta ctaccagcgg ttcgaagccg gccggcaata tccagtcctc
     1501 tgccgcaagc cggtctgcga aggggcgcca gagcaggttc ttctgaatct caatcagctc
     1561 gcccctcgtc cgcatctccg cctgggtgcc agcgaaataa gtcctgaaca tggctatcta
     1621 gccttcgcgc tcgacttttc cggtgacgag gcccacacct tgtttattcg tgatcttacg
     1681 gccggctgtc tgctgagtga gaacatctgc ggcacggcca atacgctcgc gtgggccgat
     1741 gagcgcacgc tcttctacac ggtcgtggac aaggtttccc ggccgtgggc cgttttccgc
     1801 caccgcctgg gtaccgacgc gacgacagat gtgatggtgt atgccgaggt agatgacgcc
     1861 ttctcggtgg aggtgagccg ggctcgagga ggcgagtgga tcctgatcga gagcgcgagc
     1921 gggggtagca ccgagatccg cagtatcccg gcccaacgcc cgtttgaagc gccgcgagtt
     1981 cttctgcccc gcgagccggg agtcgaatac gcggtcgcac cgctcggcga tcgactcttc
     2041 atcctcacca atgaagcggc ggagaacttc cggctggtgg aagcctcgct cgcgaatccg
     2101 aatcggacgg aatggggcga gatcctgccc cactcggcgt cggtgaagct cgacgcgatc
     2161 gatgtattcc gggatcacct ggtcgtatgg gagcgtgagg ccgggttacc ctgggttcgg
     2221 gtgctggacc tcgccaccgg ggctcaacac cgagtcgagc tacccgagcc ggccaatgcg
     2281 atctcccgca gcagcaatcc ggagctcgac accaccaccg tgaggttcag ctgtacttcg
     2341 ctcgtgagtc cattggcggt catggactac gacatggcaa cgcgcaccct cacgctgcgc
     2401 aagcaggcca ctgttcacgg gtacgaccgc actcgctatc gggctgatcg gttgaccaca
     2461 gaggcgcccg acggatctcc ggtgccgatt tcgctgatct accggtggcc ttttcccctc
     2521 gacggcagcc gccccgggct tctgctggga tatggggcgt atgggacagc ctttgaggca
     2581 ggattccagt ctcatttcct gagcctgctc gatcgcgggt ttgtggttgc gatcgcgcac
     2641 gtccgaggag gggacgagtt ggggcgcaac tggtatcaac aggggaagct cgagcgcaag
     2701 cgaaacacat tcaccgactt catcgccgca gccgagtacc tgatcgccga gggctatacc
     2761 tcggctcatc ggctggccat caacggtgcc agtgcgggcg ggatgttgat gggcgcagtc
     2821 accaacctgc ggccggagct gttccgcgtc gtcgtcgcag aggttccgtt cctagacgtc
     2881 gtcaacacga tgctcgatcc ctcgctaccg ctcacaatca cggaatacga cgaatggggt
     2941 gacccgagag acgcagcgac gtatgcctac atgcgcagct actcgcccta cgacaacatc
     3001 cagcaaaagg cgtatcccca catgctggtt accggcgggc tcaacgatca gcgggtgcca
     3061 tactgggaac cggccaagtg gacggccagg cttcgggccc gcaagaccga caacaatcgg
     3121 ctattactcc ggacgaacat gggcgccggt cacgcaggag cctccgggag atacgacgtg
     3181 atccgggagg tggccttcaa gtacgccttt attctggatg tgctcggtgt ttcctgagct
     3241 aacggattcg gcgatagata ttcgagtagt cgtcggtcca tagcgcgccc ggcacggctt
     3301 cgaaccgctc ccaccgcgga tctcgcgtaa ttctccccag atcctcttcc cgcctcgcca
     3361 gcacagccca cgtggacccg aacgcgccgc ggtagatctg tgcgagcggg ggttcctgac
     3421 cccacacccg gcccaccagg cccgcgtccg ccgcgagagc cgcaacgacg ggcgggaaat
     3481 tgatgtagcg gttgctgagg tgaatggcca gcaggccacc cggagcgagc cgctggaagt
     3541 agagccggac ggcttcccgg gtgagaaggt gaatcggcgg cgcgtccgag ctgaacgcat
     3601 ccagaatcat gacgtcgtag cgagcggtcg agtcgtgcgc cagcgagatg cgcgcgtcgc
     3661 ccatcacgat gcgtggcggg ctccgggcgt cgaccaggaa ggtgaacagg gtggtgtcgc
     3721 gagcgatccg ctctaccagg ggatcgatct cataaaaggt ccatgattcc cccggaccgg
     3781 cataagccgc agtcgtccct gctcccagtc ccaccacgcc cactcgacgc cccggcttcg
     3841 gcggagcgac tcccagcatg atgtagccga gcggtccttc tgggtgatag tagctggtgg
     3901 gcaacctccg ccaggggggg aatcggctct gcagtccgtg gatggtggtg ccgtggtaaa
     3961 ggacgcggaa gccgcctcca cgggtgtact gcactcgata gaccccgaag aaatcgcgtg
     4021 ccaggtagat caggccggga tgtcgacgta cgactgccca atgcagcccc accgccagag
     4081 ggagccccaa gccgagcatt aggtgaaatc gccggcgcca gaggagaagc gcgccgaaca
     4141 tcgcagcgat ggccgccgca ttcaggaacc agagcgatcg cagattgagc ggcagcacgc
     4201 gacgaacctc gagtgccacc gcggtgtggc ccagtacgac ggccagcgcg accatggaca
     4261 gaccgatcgt taccacgccg gcagcaccgg tcaggatagg agtccaggtc gcgagcgccg
     4321 cggttgccgc aatagcgagc gggtattccg tgacgttcga gaacaccacg ggggctacca
     4381 gaacgttgaa cgcaccgccc agtgccccgc ccagcgcgac ccagaggtaa aacgcggtca
     4441 gccgctcggc cggcggcctg agtcgagcca actggccgtg gcacagcagc ccggccacga
     4501 aaagcatgcc atagttgaga gccaggctgg tccggagcga cgcctcgccc atcacggctg
     4561 ccaccagctg aaggaacagg agcggcagcc agtgttgtgc gatgcgcggg gaaatccagg
     4621 gatgccgagc gaatgtcgcg acgaaggaca ggagatagag cgcgagcggc agtacccaga
     4681 ggagcggcac tgccgcgatg tccgtactga ggtgagttgt cactcccagc agcaagctgg
     4741 acggcaccgc ggccaggagg agccaccgcc agggcttggc ctttggctcc gatggcgcag
     4801 gcggcgccgc cttcgtggcg gccttctttg aaggctcagg attggactgc ctcacggccc
     4861 agagcccgac caatccgatc gcgacgatca gcgcgatgta gccgaccgtc catgccgtgc
     4921 gctgagaaga gagccccagc catggctcga tcagaaaggg atacgccagc aatccggtga
     4981 agcttcccag attgctggcg gcatatagaa agtaggggtc ggacgcgtcg ggatggtcca
     5041 gccggctgaa ccagcgctgc aacagaggag cggttgcggc gagaagaatg aacggcgcac
     5101 cgactgttcg gagcagcgcg accagcagcc aaccgatcgg gctctggccc gattgcggga
     5161 ggtcgttggc cggcagggcg accggcagcg tagcgagcgc caccgcgagc agtcccaggt
     5221 gcagccacac ctgcgggcgc agggcgagct gcgcctggat gaggtgcgca tacaaatagc
     5281 ccgcaagcag tgcgacctga aagaacagaa gacaggtcgt ccagaccgcg ggcgttcccc
     5341 ccagcatcgg caccgccatc ttgccgacca tgggctcaac tacgaacagc aaccccgcac
     5401 tgaggaacac cgcgagcgcg tacaggacgc gcgtggcgcg gtgccgatcc tcagcgggca
     5461 actaccatct cgcgggagaa accggcgttg cccgaaaccc ggggtggtta ggcggttccc
     5521 accccggcgc gtccgagcca ccgatcgagc aacccttccc agcccagcgt ctgccggatc
     5581 tctcccgggc gcgcgccgaa cagccgcacg gcctgccggc tgagcgagga gtgatcgctg
     5641 tagccgcact cgacggcgat ggtgagcagc gacgtctgcc gctcgcgctg gagccgaagc
     5701 gcggcgtaca ccgcatgggc agcggcgagc cacgcgccgg gaccaggaag cccggcacgc
     5761 tggaaccagt tccgcacagt gcgctcgggc cggcccagcg actggagcaa gccatgcagc
     5821 cggggatgcg acggagcctg gcggaagatg tccagaatga gattccggag ggggtccggc
     5881 gggtccacac cgcgcacggc gagccagtct tccagctcgt gcgtgagact ggcgggatcc
     5941 gtgagaaccg tctgcagggt ctcgcgaggc ggggcgtcgt cgcccaacac cgcccgaatg
     6001 tggaacggtg cggcgatgcg agccgcctcc gccgggtcga tgccgacccc gctccggacc
     6061 cggagcgcaa ggggagccgc cggaaatcgc gcgcgaagcc gcggcgcgag tgcttcgaca
     6121 gccggccagt cgttggagct ggtgagctcg atgacgagca cggagcccag gggaagatct
     6181 gcttctgacg catccaggtc gggaagttcc tcgaatcggt tataaggagg cgtcaatcgg
     6241 aaaagggcga ggggccgccc ggagtgctct ggttgcatag ccgggtcctg cgaagaagga
     6301 agagtgcgcc atggtagccc ggggcaagcg aacggctaga gggaaggggc ttacgggtgt
     6361 cctatccgct gtctcctgct cgctgcctcg cggcgaaatt gcgtccaccc aagctagtat
     6421 agtaggcaaa taatggtagc cggtcgggcc aatctggctc gaaacccctg atagctgata
     6481 acccatagct taccccatgt ccaccgatca atccatccac cgcgtatgcg tcttcgcggg
     6541 atcgaatacc ggcgcccggc ccgactatgt cacggccgcc cggcggttgg gcgaggtgct
     6601 cgccgccgaa ggactcgacc tggtctatgg tggcgccagc gtagggctta tgggcgagct
     6661 cgcggcggcg gtcct
//
LOCUS       DASRJQ010000410         3636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1780721, whole genome shotgun sequence.
ACCESSION   DASRJQ010000410 DASRJQ010000000
VERSION     DASRJQ010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1780721"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..648)
                     /gene="ispF"
                     /locus_tag="VFB89_09205"
     CDS             complement(172..648)
                     /gene="ispF"
                     /locus_tag="VFB89_09205"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HZM27523.1"
                     /translation="MTRVGIGYDSHRFVAGRPLVLAGITIPHSHGLAGHSDADAVAHA
                     LTDAILGAAAAGNIGRLFPDTDPRWKDADSMELLATAHELVRTRGLTLWQADLTIIAE
                     QPKLDRHLDAMAAALAGRLGVTAADVSVKAKTNEGMGFVGRGEGVAVIAVATLEAR"
     gene            complement(710..1768)
                     /gene="mqnC"
                     /locus_tag="VFB89_09210"
     CDS             complement(710..1768)
                     /gene="mqnC"
                     /locus_tag="VFB89_09210"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HZM27524.1"
                     /translation="MSVVDRTSSEFTSYLELYDQADLLELGSLADAERWRLHPERVVT
                     YIIDRNINYTNVCVADCQFCAFYRRPKHAEGYVLSFEEIGRKIDECKAVGGVQILMQG
                     GHNPYIPFEWYLDLMRYIKRNHPIHIHGFSPSEVVFFSERFGLSVPDVIRELRAAGLD
                     SIPGGGGEILVDEVRQRVARKKAQTEEWLGVQEEAHRQGMKTSVTMMYGLGESNADRI
                     EHLFRVRELQARTGGFTAFICWPLQPEGTPGMSHYRKTDAVTYLRTLAIARIVLDNVP
                     NIQSSWVTMGHKVGQVALRFGANDYGSLMMEENVVSAAGTTYRTTLAEIERLITDAGY
                     EPKRRRQDYSVIEEPVAA"
     gene            complement(1781..2602)
                     /locus_tag="VFB89_09215"
     CDS             complement(1781..2602)
                     /locus_tag="VFB89_09215"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HZM27525.1"
                     /translation="MRLGRIPWINCYPVYGAIDRGIVPIGAELVTGTASELNDLLAAG
                     ELDVSAVSAVEYARNAAAYHLLPDLAITCDGPVHSVALFSRRPVEQLSDATVLLSASS
                     RTSILLLELLCRHRWKIAPRFATVRAEAMDLESLAGLPHEAVLVIGDAALLLTARGDY
                     PIKLDLGAEWQSWTGLPFVFAVWAARRSAPAEPVRAVHHSLLESRAWGLSHLDELAAD
                     AARRTGVAESVCRAYLGDLDYALSYRHLAGLTDFFRRLAQDGLVPDGSLSFISAA"
     gene            complement(2599..>3636)
                     /locus_tag="VFB89_09220"
     CDS             complement(2599..>3636)
                     /locus_tag="VFB89_09220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HZM27526.1"
                     /translation="RRNGDRVFFSANQHINPTNVCVLRNTCVFCSFARMPKEEGAYTR
                     SLEEVYAEAEQARGAPTREFHIVGGLHPKLRLSYYTDMIRGLKERHPEIHIKALTAVE
                     IAHLARIEKTTAREVLLALKEAGLTSLPGGGAEVFSTAVRATIAERKLTGQEWLEVHR
                     VAHKLGIPTNCTMLYGHVETAADRIEHLSMLRELQDETGGFLTYIPLAYHPDHNELGE
                     ELGRVGTATTGFEDLRAIAVGRLFLDNVPHVKTHWPMVTPFLSQIALAFGCDDVEGTV
                     IYERIYHEAGAHTDMGMPYLELVRLIRGAGKRPVERDSLYRALRESFDDPPPTEPAVR
                     RGASLPVVHAA"
BASE COUNT          667 a         1168 c         1163 g          638 t
ORIGIN      
        1 gaaacacaat cggaggcgag gtgacgccct tgtgcgatag aaacgctccg agcaggacgg
       61 caaggtcggc gggcacaggt ggaaggatgt tttcgatcac cgagatcgcc gccagaaacg
      121 cgtatacggc gaccggcggt aaccccgtga gccagtcgag aattgcgtcg atcagcgggc
      181 ctccagcgtc gccactgcga tgaccgccac accttcgccc cgtcccacga atcccatgcc
      241 ctcgttagtc ttggccttca cactcacgtc cgccgccgtc acgccaagcc ggccagccag
      301 ggcggcggcc atggcgtcca ggtgacggtc gagcttcggc tgctctgcaa taatcgtgag
      361 gtcggcctgc cagagcgtca gcccgcgagt gcggaccagc tcgtgagcgg tcgcaagcag
      421 ctccatcgaa tccgcatctt tccagcgggg atccgtgtca gggaagagtc gcccgatatt
      481 gcccgcggcg gcagcgccca gaatcgcgtc ggtaagcgca tgcgcgacgg cgtcggcatc
      541 ggagtgaccg gcgagaccgt gcgaatgagg gatggtgatt ccggcgagca cgagggggcg
      601 gccggcaacg aaacggtgcg agtcgtagcc gatgccgacg cgggtcacga acagtcgcct
      661 gtcgtccgct ggcgcccgcc cgctagagta gggtgtcgcc gactcctggt catgccgcca
      721 ccggctcctc gatgaccgag taatcctgtc gacggcgctt gggctcgtag cccgcgtccg
      781 tgatcaggcg ctcgatctcc gccagagttg tacggtaggt ggtgccggcg gccgaaacga
      841 cgttctcctc catcatgagg ctgccgtagt cgttcgcacc gaatcgcaag gctacctgac
      901 ccaccttgtg gcccatggtg acccaggaag actgaatgtt cggcacgttg tcgagcacga
      961 tccgcgcgat cgcgagggta cgcagatagg tcacggcgtc cgtcttccgg tagtgcgaca
     1021 tgccgggggt gccttccggc tgaagcggcc agcagatgaa tgcagtaaag ccgccggtgc
     1081 gggcctggag ctcacggacc cggaagagat gttcgatccg atcggcgttg ctctcgccca
     1141 gtccgtacat catggtcacc gaagtcttca tgccctgtcg gtgcgcttcc tcctggacgc
     1201 caagccattc ctcagtctgg gccttcttcc gggcgacccg ctgccgcacc tcgtccacca
     1261 ggatctcgcc gcctccgccg gggatgctgt ccaaccccgc tgccctcagc tcgcggatga
     1321 cgtcgggcac cgaaagcccg aaacgctcgc tgaagaagac cacttcggac ggtgagaaac
     1381 cgtggatatg aatcgggtgg ttgcgcttga tgtagcgcat caggtcgagg taccactcga
     1441 acgggatgta gggattgtgc ccgccctgca tcaggatctg gacgccgccc acggccttac
     1501 actcgtcgat cttgcggccg atctcctcga agctcagcac gtaaccttcg gcgtgcttgg
     1561 gccggcgata gaaggcgcag aactgacagt cggcgacgca gacgttggtg tagttgatgt
     1621 tccggtcgat gatgtaggtg accacccgct ccggatggag ccgccagcgc tcggcgtcgg
     1681 ccaaggagcc caactcgagc aggtcggcct ggtcgtatag ttcgaggtag cttgtgaact
     1741 cgcttgaggt ccgatcgaca acgctcatat aatcagttac ttacgccgct gagataaagg
     1801 aaagtgaccc atcgggcacc aacccgtcct gcgccaaccg acggaagaag tcggtgagcc
     1861 cggccagatg tcgataggag agcgcgtagt cgagatcgcc cagataggct cggcacacac
     1921 tctcagcaac gccggtccgg cgtgcggcgt cagcagccag ctcgtcgagg tgcgaaagtc
     1981 cccatgcccg cgactcgagc agactgtgat ggactgcgcg aaccggctca gctggagccg
     2041 agcgtcgcgc cgcccacaca gcgaagacaa agggaaggcc agtccaagac tgccactccg
     2101 ctccgagatc cagcttgatc ggatagtctc cgcgtgctgt cagcagcaga gctgcgtcgc
     2161 caatgaccag cactgcctcg tgtggcagtc ccgccaaact ctccaggtcc atggcctcgg
     2221 cccgaacggt cgcgaatcgc ggcgcgattt tccagcgatg gcgacagagc agctcgagga
     2281 gcagaatcga cgtccgcgac gacgcagaga gcagcaccgt agcgtccgag agctgttcca
     2341 ccgggcgccg gctgaacagc gcgacgctat ggaccggccc gtcgcaggtg atggcaagat
     2401 cgggcagcag atgatacgcg gcggcgtttc gcgcgtactc gacggcagat actgcgctca
     2461 cgtcgagctc accggccgcg agaagatcgt tcagctccga cgcagtcccg gtgaccagct
     2521 ccgccccgat cggcacgatg ccacgatcga tggcaccgta cactggatag caattgatcc
     2581 aggggattcg gcctagtctc atgcagcgtg taccaccggc aagcttgcac cgcggcgcac
     2641 cgccggctcg gtcggcggcg ggtcgtcgaa gctctctcgc aacgcgcgat acaggctgtc
     2701 ccgctcgaca gggcgcttcc ccgccccgcg aatcagtcgc accagctcga gatagggcat
     2761 ccccatgtca gtgtgagcac cagcttcgtg atagatccgc tcgtaaatga cggtcccttc
     2821 tacgtcatcg cacccgaacg cgagcgcgat ctgcgagagg aacggcgtca ccatcggcca
     2881 gtgggtcttc acgtgcggga cgttgtcgag gaagagccga ccgaccgcga tggccctcag
     2941 atcctcaaac ccagttgtcg cggtgcccac tctgccaagc tcttcgccca gctcgttgtg
     3001 gtccgggtga tacgccagcg ggatgtacgt gaggaagccg ccggtctcgt cctgcagctc
     3061 tcgcagcatg ctgagatgct cgattcggtc ggccgcggtc tcgacgtgcc cgtagagcat
     3121 cgtgcagttg gtcggaatgc cgagcttgtg ggccacccta tggacttcga gccactcctg
     3181 gccggtgagc tttcgctcgg cgatggtcgc acgcaccgcc gtgctgaaaa cctcggcccc
     3241 gccacctgga aggctggtaa gcccggcctc cttgagcgcc aggagcactt cacgcgcggt
     3301 ggtcttctcg atccgtgcca ggtgcgcaat ttcgactgca gtcagcgcct tgatgtggat
     3361 ctcgggatgc cgctccttga gcccgcggat catgtcggtg tagtagctga gccggagctt
     3421 ggggtgcagt ccgccgacga tgtggaactc gcgggttggt gcgccccgtg cctgttcggc
     3481 ttccgcgtac acctcctcga gcgacctggt gtaggcgccc tcctcctttg gcatccgggc
     3541 gaaggaacag aagacgcaag tgtttcgcag gacgcagaca ttggtgggat tgatgtgctg
     3601 attcgccgag aagaagaccc ggtcgccgtt gcgccg
//
LOCUS       DASRJQ010000411         3282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_983896, whole genome shotgun sequence.
ACCESSION   DASRJQ010000411 DASRJQ010000000
VERSION     DASRJQ010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983896"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            272..520
                     /locus_tag="VFB89_09225"
     CDS             272..520
                     /locus_tag="VFB89_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZM27527.1"
                     /translation="MNDGPEMILLKIEGMLTQESLPVLEEELRRALAVAERVRLDLRD
                     VVYIDPPGVGALRRLPRDRAEIVDCTPLIKDLLSKESA"
     gene            517..1104
                     /locus_tag="VFB89_09230"
     CDS             517..1104
                     /locus_tag="VFB89_09230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZM27528.1"
                     /translation="MTTSLMPDGRAREFEALLVPVLEPAYRAAFHLARNRPDAEDIVQ
                     EATLLAWRSFDRFEPGTNFRAWFLRIVTNAFLSRYRAERRRGATISLDQETSEGTDRT
                     AAEIWRVAGSADSTEAILAKLALEDVQAALASLPIEYRTVAVLYFVDDLSYEQIATAV
                     GCPIGTVRSRLHRARRLLWSRLVSTARDMGMAAAV"
     gene            complement(1147..1281)
                     /locus_tag="VFB89_09235"
     CDS             complement(1147..1281)
                     /locus_tag="VFB89_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27529.1"
                     /translation="MNRHAEKLILTVGSSIPIDPALGGYIEEMHGCPNCGNVESRRPD
                     "
     gene            complement(1344..1928)
                     /locus_tag="VFB89_09240"
     CDS             complement(1344..1928)
                     /locus_tag="VFB89_09240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HZM27530.1"
                     /translation="MSTEATGPDRPLRIPVILGTARKGRMSAHAAQFVLGELRKRAGV
                     ATDLIDIATLPVRLDQSGQDTADPAFGESVRRADAIVLVIPEYNHGMPGLLKHVLDSC
                     LKEYIHKAVGIVAVSAGPFGGTRVIETSLPVLRELGLVTIFWDVNVGSVERVFGASGE
                     LLDQAFVRRTDKFLGELIWMTRTMRHGREHIPLQ"
     gene            complement(1925..>3282)
                     /locus_tag="VFB89_09245"
     CDS             complement(1925..>3282)
                     /locus_tag="VFB89_09245"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HZM27531.1"
                     /translation="SKWQVIDPTTLDRRRSHGRLAFNWYRTSVTLPQKVGSLDVTGAT
                     VIFELVIDDYAEIWVNGKLPLVLGQTGGQLIKGFNAPNRVVLTTDARPGQRFQLAIFG
                     INGPVSSPPVNFIWIRSATLDFYRKGAVGGGQDAGGRIVRLDPSLDSIVPPGAKLEKL
                     AGGFQFTEGPVWHPDGYLLFSDPNANTIYRWSPEGAVSVFRTKSGYTGFDIGEYHQPG
                     SNGLTLDPNGLLTINEHGNRRVVRLERPGNITVLADRYEGKRLNSPNDLVYRSDGTLY
                     FTDPPFGLPKAFDDPRKELPFSGVYMVKGGQVRLLIKGLSGPNGIAFSPDENYLYVDN
                     WDLQRKVLMRYEVNADGSLSNGKVFYDFTSEPEEVALDGIKVDQRGNVYLSAPRGIWI
                     LSSEGKPLGRIEPPEHDANMAWGNSDGRTLFLTASTGLYRIRLNIPGVRPPLGVQSAS
                     Q"
BASE COUNT          591 a         1015 c         1043 g          633 t
ORIGIN      
        1 tcaagcctac cactctcgag gcgcgcatga agaagcttgg agttcgacga cccggctagt
       61 cccaacctag tcggggacgg acttgggaaa gtcccaatac gttgggaccg tatcctccca
      121 ccgcagtcca ttagaaaacc aatccagcgg cgcaactcgt ttaaacaaca gcagttcgat
      181 cgacactgcc aggcttcccg gcagtggcac gtgtggtgct ttttgtctgg tcagaccgct
      241 tcagacgaat gaaatgctga gactaacgcg aatgaacgat gggcccgaga tgatcctgct
      301 caagatcgaa gggatgctca cccaggagtc gttgccagtg ctcgaagaag agcttcgccg
      361 cgcgctcgct gtcgccgaac gcgttcggtt ggaccttcgt gatgtcgttt acatcgaccc
      421 tccgggtgtc ggcgcgctgc ggcgcctccc gcgggaccgt gccgagatcg tggactgcac
      481 accgctcatc aaggacttgt tgagcaagga gtctgcatga caacgagcct catgcctgac
      541 ggccgtgcac gagaattcga ggcgttgctc gtgccggtgc tggagcccgc gtaccgcgcg
      601 gcgttccatc tcgcgcggaa tcgaccggac gctgaagaca tcgtccagga agcaacgctg
      661 ctcgcctggc gcagcttcga caggttcgag ccgggaacga acttcagagc ctggtttctc
      721 cgtatcgtca ccaacgcgtt cctgagccgt tatcgcgccg agcgacgccg gggggccaca
      781 atctcgctgg accaggagac aagcgaagga accgatcgaa cggcggcgga gatctggcgg
      841 gtcgccggca gcgcagactc gaccgaggcg attctcgcca agctcgctct ggaggatgtt
      901 caggccgccc ttgcgtccct tccgatagag taccgcactg tcgccgtgct ctacttcgtg
      961 gacgaccttt catacgaaca aatcgcgacg gcggtgggtt gtccaattgg cacggtgcgc
     1021 tcgcggcttc atcgcgcgcg ccggctgctc tggagccggc tcgtcagcac cgcacgtgat
     1081 atggggatgg cggctgccgt ttgaggcctg acgacggccc ggatcgatct acctgaaagt
     1141 ggaaccctaa tccggccgac gcgactcgac gttgccacaa ttagggcagc cgtgcatctc
     1201 ctcgatgtat ccgccgagcg ccgggtcgat cgggatcgac gagcccaccg tcagaatcag
     1261 cttctccgcg tgccggttca tgattgtgcc acaccggggg caccgcatag gtgctcgagc
     1321 ttctgtcact gggtctttcc tatctattgc aggggaatgt gctcgcgccc gtggcgcatt
     1381 gtcctggtca tccagatcaa ctcccccagg aacttgtcgg tacggcgcac gaatgcctga
     1441 tccagtagtt caccgctggc gccaaacacc ctttcgacgc tcccaacgtt cacgtcccag
     1501 aagatggtca ccagcccgag ctctcggagc acggggaggg aagtttcgat caccctcgtc
     1561 ccgccgaagg gacccgccga caccgccacg atcccgacgg ccttgtggat atactccttg
     1621 aggcagctgt cgaggacgtg tttgagcagg cccggcatcc cgtggttgta ttccgggatc
     1681 acgagaacaa tggcgtcagc gcggcgcaca ctttcgccga atgcggggtc tgctgtgtcc
     1741 tgcccgcttt ggtccagccg cacggggagg gtcgcgatgt caatcaggtc ggtcgccacg
     1801 ccggcccgct tccgcaactc accaagcacg aattgcgcgg cgtgggcgct cattcttccc
     1861 ttgcgggcag tccccaggat caccgggatc ctgagcgggc ggtcgggccc ggtggcttct
     1921 gtgctcattg gctcgccgac tgtacgccga gcggcggccg cacgccggga atgttgagtc
     1981 gaatgcggta gagaccggtg ctggccgtca gaaacagcgt gcgaccgtcg gaattacccc
     2041 aggccatgtt cgcgtcatgt tccggtggct cgatcctgcc gagcggcttc ccctcggaag
     2101 acagaatcca gatcccgcgc ggcgccgaga ggtagacgtt gccccgctgg tctaccttga
     2161 tcccgtcgag cgcgacttct tcgggttcgc tggtgaagtc atagaagacc ttgccgttcg
     2221 agagggagcc gtcggcattc acctcgtagc gcatgagcac tttccgctgg agatcccagt
     2281 tgtccacgta caggtaattc tcatccggcg aaaaggcgat cccgttgggg ccgctcaggc
     2341 ccttgatgag caggcgtacc tggccgccct tcaccatgta gacgccgctg aagggcagct
     2401 ccttccgggg atcgtcgaac gccttgggaa ggccgaatgg tgggtcggtg aagtagagcg
     2461 tcccgtcgga ccgatagacg agatcattgg ggctgttcag tcgcttgcct tcatagcggt
     2521 ctgccaggac cgtgatgttg ccgggtcgtt cgaggcgaac cacccgacgg ttaccgtgct
     2581 cattgatggt gagcaggcca ttggggtcga gcgtcagacc gttcgagccc ggctggtggt
     2641 actcgccgat atcgaatccg gtataaccgc ttttggtgcg gaaaacggac accgcgccct
     2701 ccggcgacca tcgataaatg gtgttggcgt tcgggtcgct gaagaggagg tagccgtccg
     2761 gatgccagac cggtccttcg gtaaactgga aaccaccggc cagcttttcg agcttggccc
     2821 ccgggggaac gatcgagtcg agggaggggt cgagacgtac gatcctgccg cctgcgtcct
     2881 gccctccgcc cacggccccc ttgcggtaga agtcgagcgt tgcggagcgg atccagatga
     2941 agttaaccgg cgggctcgag accgggccgt tgattccgaa gatcgcgagc tggaagcgct
     3001 gcccgggccg cgcgtcggtg gtcagcacca cacgatttgg ggcattgaag cccttgatca
     3061 gctgaccgcc ggtttggccg agcacgagcg gcagcttccc gtttacccag atctccgcat
     3121 agtcgtcgat gacgagctcg aagatgacgg ttgcgccggt cacgtccaag ctgccgactt
     3181 tctgcgggag cgtgacgctg gtgcggtacc agttgaacgc caggcgcccg tgggaccgcc
     3241 gccggtccag ggtggtggga tcgatgactt gccacttgga gt
//
LOCUS       DASRJQ010000412         2345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_296261, whole genome shotgun sequence.
ACCESSION   DASRJQ010000412 DASRJQ010000000
VERSION     DASRJQ010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296261"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(756..>2345)
                     /locus_tag="VFB89_09250"
     CDS             complement(756..>2345)
                     /locus_tag="VFB89_09250"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZM27532.1"
                     /translation="HDVKMGGEYLRFHNRFIWCLRCNGVIDATASPVPANIESLFPVW
                     NDASTWNTAPLAPFTRWVYHSLSDTEHQYAIARNLFAGWVQDDWKVNDHLSLNLGVRY
                     DIDTNAHSEKVRFTPWLPGNLPHDTNNLAPRLGASYALNDRTALRGGYGLFFAFAPND
                     GVQQTEGYRHRFENQILNNGRADFTTVRDGFYGWFNGPKPTFEESLQNACDVNLRPGC
                     VYRSLTQEINYPGRQTSYSHQASGGIQRQFGTDMSLEVNYVFTGGRREETAQQVNLTY
                     NPATGANYPFTAIATRAFPQWGAVDFELLEGRSNYHGVDVTFTKRFSHRWQGSATYTR
                     SRFMDADPVRDQWYIGSDGLVARRPIGFALAPDLGGEYALAGAYSGGGVGAAGDQPHR
                     AVFNGIWDMGYGVQFSGIYFYGSGERFRTNFGSDLRDEGGTVGIIGSARLRRDGTIIP
                     LTGIVGDPIHRVDMRLQKRLSLGGRREIDGILEVFNLLNHANYGSYTINESNANYGRP
                     SSNDNLAYQPRMLQLGFRLAF"
BASE COUNT          433 a          726 c          742 g          444 t
ORIGIN      
        1 cggacgatgc ttatcgtgct cacctgtccg gcggcctcga acagtgcttg cagatcggat
       61 tcggtggttt catacggcag gttaccgacg tacagcttgg tggtcatgaa cttctcctgc
      121 atctcgacgg atgcgactga attctgaagg caggctcgag cggaatgctc gcgacgaaac
      181 gcaagagagg cgagaaactc ggatctgatg cggaaggcct aaagcgaagc ggtcagaagc
      241 tacgcacagt gtagcacggc gatccaggaa ggcttcatca gtgtcaattg ccgagcgccc
      301 gtggggacgc cacgtgttcg gttttgaaca atgtgtcctg cacgcgttcg ctagactgca
      361 ttcgtatggt gtggttccct cggccggcgc cgaagcctcg gatctctgtc gtgtgcccgc
      421 ggtgcaattc actacgcgcc ttcgtcacgt acgcgcgggt caggaccgag tgttcctttt
      481 gcccagattg tcacaacctc tgggacacca tccgacccga cagtccggct caccagtcgt
      541 cgcgatgacg acagctcccg ccagcgggcg agccgtgcca cgaaggaaca cgctcgcgcg
      601 gcgctttccc gccttgccgg ccgcgccctc atcgtcaaaa tcaatcggct gtttcgaccg
      661 taacgggtaa tgcgggcgcg cccttactga gcgcgccctt gtatcatcga acgcaatgag
      721 ctctcgagcg gctcattgac accgagcttt ccacatcaga aagccaggcg gaatcccagc
      781 tgcagcatcc gtggctgata ggcaaggttg tcgttggacg acggccggcc ataattcgca
      841 ttgctttcgt tgatcgtgta ggagccgtaa ttcgcgtgat tcaggaggtt gaacacctcg
      901 agtatcccgt cgatctccct gcggccgcca agcgacaatc gcttctggag ccgcatgtcc
      961 acgcggtgga tcggatcgcc cacgataccg gtgagcggga taatcgtacc gtcccggcgg
     1021 aggcgggccg agccgatgat cccgacggtg cccccttcgt cgcggaggtc ggagccgaag
     1081 ttggtcctga aacgctcgcc tgagccgtag aaatagatcc cgctgaactg cacgccgtag
     1141 cccatgtccc agatgccgtt gaagacggcc cggtgaggct gatcgcccgc cgccccgacg
     1201 cctcctccgg agtacgcccc ggccagcgca tactcaccac cgagatcggg cgcgagcgcg
     1261 aagccgatcg gccgtcgcgc cacgaggccg tcgctgccga tgtaccactg gtcccgaacc
     1321 ggatcggcat ccatgaaccg cgaccgggtg taggtcgccg agccctgcca gcggtgactg
     1381 aaccgcttgg tgaacgtgac gtccaccccg tggtagttcg accggccctc caacaactca
     1441 aaatccacgg cgccccactg agggaacgcc cgcgtggcga tggcggtgaa gggatagttg
     1501 gcgcccgtgg cggggttgta tgtcagattc acctgctggg cggtttcctc gcgccggcct
     1561 ccggtgaaga cgtaattcac ttccagggac atgtcggtcc cgaactggcg ctggataccg
     1621 cccgacgctt gatggctgta ggacgtctgg cgccccgggt agttgatttc ctgggtcagc
     1681 gaacgataga cacaaccggg gcggaggttc acgtcgcagg cgttctgcaa cgactcctcg
     1741 aaggtcggct tgggcccatt gaaccaccca tagaacccgt cgcggaccgt cgtgaagtcg
     1801 gcgcgcccgt tattgaggat ttgattctcg aatcggtgcc gatacccctc tgtctgctgg
     1861 accccgtcgt tgggggcaaa ggcgaagaag agcccgtacc cgccgcgcag tgccgtccga
     1921 tcgttgagcg cgtagctcgc ccccaggcgc ggggcgaggt tgttcgtgtc atggggaagg
     1981 ttgccgggca gccagggcgt gaaacggact ttctccgagt gagcattcgt gtcgatgtcg
     2041 taccgcacgc cgaggttgag cgagagatga tcgttgacct tccaatcatc ctgcacccag
     2101 cccgcaaaca ggttccgggc gatcgcgtat tggtgctccg tatccgacag cgagtggtac
     2161 acccacctgg tgaagggagc cagcggcgcc gtattccagg tggaggcgtc gttccagacc
     2221 gggaagagcg actcgatgtt ggcgggcacg ggtgacgcgg tggcgtcgat gacgccgttg
     2281 cagcgcaggc accaaatgaa ccggttgtgg aaccgcaggt actcgccgcc catcttcacg
     2341 tcgtg
//
LOCUS       DASRJQ010000413         4903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1718138, whole genome shotgun sequence.
ACCESSION   DASRJQ010000413 DASRJQ010000000
VERSION     DASRJQ010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718138"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VFB89_09255"
     CDS             complement(<1..710)
                     /locus_tag="VFB89_09255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HZM27533.1"
                     /translation="MPEEEPRVGEERLLLALLPTDTGLWEWRSGDGARYLGDAWTTRL
                     GYEPRALDGYAHLPTALIHADDLPAALGRLKAHLDGTTAWYEAEYRLRTKRGEWRWIH
                     DRGRVVERDGEGQPLRMIGMPTDVTERREAEAALFRERERALVTLASIADAVIATDSR
                     GMVEYLNPVAEHFTGWTLSEARGEPLTRVFTVLHEVTREPIETSAERCLDEDRAMGVT
                     SHLLLVRRDGAEFAIDESA"
     gene            complement(959..1147)
                     /locus_tag="VFB89_09260"
     CDS             complement(959..1147)
                     /locus_tag="VFB89_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27534.1"
                     /translation="MFEDEERLPWETDGDLPWASEEPEWEAPDPEAWRGTVHLQEWPE
                     DLAGPEYWLYKKQGEEEE"
     gene            complement(1291..3261)
                     /locus_tag="VFB89_09265"
     CDS             complement(1291..3261)
                     /locus_tag="VFB89_09265"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HZM27535.1"
                     /translation="MHRRRFLTGAARSAAAFGVLRQLGGCRKATPSAESKLASPGDAF
                     SAIRDRYFVRVLQLYPVTSTYLGGDGYDSALATTNGTLRNWSPDAIKSEAAFYREIER
                     ARQAIDPGRLEPPERIDHTVLGAQIAFMLRQLEERRYYERAVDTYIAEPFRGIDWQLQ
                     QMSATSDGQGTESEWELVVKRLESIPSYLAVAQANLTSGRQEGNLPDRRLVQHDGIAG
                     SDANDQFFRTALPATTEAYLGKRQFAPRMLAAVTRASGRAADAFHEFTEFLQRTYDQS
                     ERTNRYAAGEAEYDWRLTHCLHMPRSSAELWDYGAEQVALYESRLYEVAREVAGSAKL
                     DVDLDNASARRAAVRRIMQHLSSDSPKNDDELLRWYRDTGGRAVSYGREQHLFDVPAE
                     YRLDVVFTPPVLRSSLDAAYYPAPPFKRSGVGRFYLTPTGNDPGQLKVNNRASVADTA
                     VHEGFPGHDWHYKYMTEHAADISNIRWLTPGAVEDSSSMWEDSMAIEGWALYAEDLMA
                     EPTPGNSHGFYSAAEHLYELQGQLLRAARVRIDVGIHTGRLSFDEAVDYFTEHVQFYP
                     YACAHAARDPVAKALCQTATRAMYRYSKWPTQAVTYNLGKQEISELRDAAHQRAGAGF
                     STRAFHERLMRMGTIPVGYYRDEFLAAGQPAS"
     gene            3320..3393
                     /locus_tag="VFB89_09270"
     tRNA            3320..3393
                     /locus_tag="VFB89_09270"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3354..3356,aa:Pro,seq:ggg)
     gene            complement(3458..3898)
                     /locus_tag="VFB89_09275"
     CDS             complement(3458..3898)
                     /locus_tag="VFB89_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27536.1"
                     /translation="MFGRMHPDVFTDTWVAAWERELNASPAYREAAADWEGAVALRMS
                     ADATLGIPEPRTVWLDLYHGLCRSARSATDADLTSAKYLIEAEPAVWNELLAGRLSPI
                     GALLAGKLRLAQGSLMGLLPYAAAAQLLVATATIIQQQFPEPAL"
     gene            complement(3919..>4903)
                     /locus_tag="VFB89_09280"
     CDS             complement(3919..>4903)
                     /locus_tag="VFB89_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27537.1"
                     /translation="LTSDFSIGVGRQHFVQDSADVAIPSHAWGNSIFLGGRTGDLRIS
                     AGLYDSHTDSVSNLSSFVALGHNVTSWLDAELFVLQSRPQTRNVTTTPILSLRERVSP
                     RVGLMQQITVADGRPRIQFGGNLLTPIGEFGVDYTVVHQPFQPFSPFRSAITLMARLQ
                     LGRYSTNFGTYVQPDGTVDYAASGSTFLYMGALGGVQPQRVGGGGIARYIVRGRVLDE
                     VRQPVEGAAIDFDGETVFTNSRGEFLLRLRQPRRLVPQVLLNDFLLPGRWQVTAVPER
                     VQAEPEDRAHPIEIRLERAVPVPVLPESVRPEPSDTLLPLVPPAGGGSAKP"
BASE COUNT          854 a         1559 c         1576 g          914 t
ORIGIN      
        1 gccgactcgt cgatggcgaa ctccgcgccg tcgcgtctca ccaggagcag atggctcgtg
       61 acgcccatgg cgcggtcttc gtccaggcat cgctcggcgg acgtctctat cggctccctg
      121 gtaacctcgt ggaggacggt aaagacccgt gtcagcggct cgcctcgtgc ttcggaaagg
      181 gtccagccag tgaagtgttc tgcgaccgga ttcagatatt cgaccatgcc ccgactgtcc
      241 gtggcgataa cggcgtcggc gatggatgcc agcgtgacga gagcacgctc gcgttcgcgg
      301 aacagagcag cctcagcttc gcgccgttcg gtaacgtcgg tcggcatgcc gatcatccga
      361 agcggctgcc cttcgccgtc gcgctcgacc acgcggccgc ggtcgtgaat ccagcgccac
      421 tcgcctcgct tggtgcgcag gcggtactcg gcttcgtacc aggcggtggt gccgtcgaga
      481 tgcgctttca ggcggccgag ggcggccggt aggtcatcgg catgaatcag tgctgtgggg
      541 aggtgagcat agccgtcgag ggccctcggc tcatacccga gtcgtgtcgt ccacgcgtcg
      601 cccagatacc gggcaccgtc gccggagcgc cattcccaca gaccggtgtc ggtcgggaga
      661 agcgcaagca gcagccgctc ttcgccaacg cgcggctcct cctcgggcac gcgctgaggc
      721 ggaggcggcg cctgaggcga cgcatgggtt cgcgatgttc gtgtcgtcac ggatgcgggc
      781 cccgggccca agtgggaatc agccaaatat gtgccgcgga tcagacagtc ggcgaggccg
      841 acccgaatac atgccgaacc tagggtagtg ccaaccgtag aacgccgaac ggtgaacgac
      901 tccgcgcaaa taattcgcgg gcgtcattca ccgtttattt ttcacgactt actgcagatt
      961 actcctcttc ctcaccctgc ttcttgtaga gccagtactc cgggccggcc agatcctccg
     1021 gccactcctg aaggtggacg gtgccccgcc aggcttcagg gtccggggct tcccactccg
     1081 gctcctctga tgcccagggg agatctccat cagtctccca aggcaaacgt tcctcgtctt
     1141 cgaacatcgc accacctccg tcgaggctct gcggccttca caaatgcagg cctcgtgccg
     1201 cgttgcaggc ggtggccgaa atggccacgg tccttcttga ggctaatgca aggttctcga
     1261 acggattgac gagccacgag cgggcgagcc tcagcttgcc ggttgccccg ccgccaaaaa
     1321 ctcgtcccga tagtaaccca ccgggatcgt gcccattcgc atgaggcgct cgtgaaatgc
     1381 gcgcgttgaa aagccggcgc cggctcgctg gtgggcggcg tcgcgcagct cgctgatctc
     1441 ctgctttccc agattgtagg tcaccgcctg agtgggccac ttggaatagc ggtacatggc
     1501 acgcgtcgcc gtttggcata gcgctttggc aacgggatcg cgagcggcgt gagcgcaggc
     1561 ataggggtag aactgaacgt gttcggtgaa gtaatccacc gcctcgtcga aggacagccg
     1621 gccggtgtga atcccgacat cgatccggac ccgggcggcc cgcagcagtt ggccttgcag
     1681 ctcgtacaag tgctccgccg cactgtagaa cccgtgggag ttgcccggcg tcggctccgc
     1741 catcaagtcc tcggcataga gcgcccagcc ctcgatcgcc atcgagtctt cccacatcga
     1801 cgaggaatct tccactgcgc cgggtgtcag ccatcggatg ttggagatgt cggcagcgtg
     1861 ctcggtcatg tacttgtagt gccagtcatg tcccgggaat ccttcgtgga cggctgtgtc
     1921 cgctactgag gcgcggttgt tgactttgag ctgtccgggg tcattcccgg tcggcgtgag
     1981 atagaagcgg ccgactccgc tgcgtttgaa cgggggagcc gggtagtagg cggcatccag
     2041 tgagctgcgg agcacgggcg gtgtgaagac cacgtccagc cgatactcgg ctgggacgtc
     2101 gaacaggtgt tgctcccggc cgtagctgac agcgcgaccg ccggtgtcac ggtaccaacg
     2161 cagtagttcg tcgtcgtttt tcggtgagtc gctcgacagg tgctgcatta tccgacgcac
     2221 cgcagctcga cgtgccgatg cgttgtccag gtcgacgtcg agctttgcgc tgccggctac
     2281 ttcccgcgcc acctcgtaga gcctggactc gtacagcgct acctgctcag ctccgtagtc
     2341 ccagagttca gcgctgctcc ggggcatgtg caggcaatgc gtcaggcgcc agtcgtactc
     2401 ggcctcgccg gcagcgtagc ggttggtccg ctccgactgg tcgtaggtcc tttgcaggaa
     2461 ttcggtgaac tcgtggaacg cgtctgcggc ccggccactc gcgcgagtca ccgcagccag
     2521 catacggggc gcgaactgtc gcttgcccag ataagcctct gtcgttgcag gcagcgccgt
     2581 tcggaagaac tgatcgttcg catccgagcc ggcgattccg tcgtgctgca ctagacggcg
     2641 gtctggcagg tttccttcct gtcgcccact agtgaggttg gcttgggcaa cggcgagata
     2701 ggaggggatc gactccagcc tcttcaccac caactcccat tcggactcgg tcccctgtcc
     2761 atccgatgtc gcggacatct gctgaagctg ccaatcgatg ccacgaaaag gctcggcgat
     2821 gtaggtgtcg accgctcgtt cgtagtatcg ccgctcctcc agttgccgga gcatgaaagc
     2881 gatctgggcc cccagcacag tgtggtcgat cctctccggc ggctctaggc gaccggggtc
     2941 gatcgcctgt cgggcccgct cgatctccct atagaaggcc gcctccgatt tgatggcgtc
     3001 cggcgaccag ttgcgcagcg taccgttcgt cgtggcgagc gccgagtcgt agccgtctcc
     3061 accgagatag gtcgaggtga ccgggtaaag ctggaggact cgaacgaagt agcggtcccg
     3121 gatggcgctg aaggcatcac cgggtgaggc gagcttgctc tccgccgacg gcgtcgcctt
     3181 gcggcatccg ccgagctggc gaagcactcc gaaggcggca gcactgcggg cggcgccggt
     3241 gagaaagcgg cgtcgatgca tggacgaata taccccttcc gtccgatgct accgagggct
     3301 acacttcgcg tccgcatcgc gggacgtggc gcagtccggt agcgcactgc aatggggttg
     3361 caggggtcgc gggttcaaat cccgccgtcc cgattcggcg gccggtcgaa tgaccggccg
     3421 cttcgtttgt tggtggcgtt agttgaggag cggtgtccta gagcgcgggc tcgggaaact
     3481 gctgctgaat gatggtggcc gtagcaacca gcaactgtgc tgcggcggca tacggcagaa
     3541 gacccatcag acttccctgt gccagtcgca gctttcccgc caggagcgcc ccgataggcg
     3601 acaagcgccc ggcgagcagc tcattccaaa cggcgggctc ggcctcgatg agatatttcg
     3661 cgctcgtgag gtcggcgtca gtcgccgaac gggcgctgcg acacagcccg tgatagagat
     3721 cgagccacac ggtccgaggc tcggggatac ccagcgtcgc atcggcgctc atccgcagcg
     3781 ccacagcgcc ttcccaatct gcggctgctt cccgataagc aggactggcg ttgagctccc
     3841 gctcccaggc tgcgacccac gtatcggtaa acacgtctgg atgcatgcgg ccgaacatta
     3901 tacgcgcctc cgcttcgctc aaggtttggc cgacccgccg ccggcaggtg gcacgagcgg
     3961 caggagagtg tcggatggtt caggacgaac gctctcaggc agcaccggaa cgggcacggc
     4021 gcgctcgagt cgaatctcga ttggatgagc ccgatcctcg ggctccgcct ggacccgttc
     4081 gggaactgca gtcacctgcc accgtcccgg cagcaggaaa tcattcaaca ggacctgcgg
     4141 caccagccgg cgtggttgcc ggaggcggag caggaattcg ccacgtgaat tggtaaaaac
     4201 ggtctcaccg tcgaagtcga tggcggctcc ttcgaccggt tgccgcactt catccagaac
     4261 ccgaccccgg acgatatacc tggctatgcc cccgccgccg acccgctgcg gctgtacccc
     4321 accgagcgct cccatgtaca agaatgtgct gccggacgcg gcatagtcca ccgtgccgtc
     4381 cggctgcaca taggttccga aatttgttga gtaccgtccg agctgcagcc gcgccatcag
     4441 ggtgatggcg ctgcggaacg gactgaatgg ctggaagggc tgatgtacga ctgtgtaatc
     4501 caccccgaac tcgccgattg gcgtaagcag gttcccaccg aattgaatcc ggggccgccc
     4561 gtccgccacc gtgatctgct gcatcagtcc gactcggggt gagactcgct ctcgaaggct
     4621 gaggatcgga gtcgtagtga cattccgggt ttgcggacgg ctctgcagta cgaacagctc
     4681 ggcatcgagc caggatgtga cattgtgccc gagcgcgacg aaagagctca ggttgctcac
     4741 actgtcggtg tgcgagtcgt acagtccggc tgagatccgc aggtctccag tgcgcccgcc
     4801 gagaaagatc gaatttcccc atgcatgact tggaatcgcg acatcggctg agtcctgaac
     4861 gaagtgctgc cgcccgaccc cgatcgagaa gtccgaggtg agc
//
LOCUS       DASRJQ010000414         2853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3488389, whole genome shotgun sequence.
ACCESSION   DASRJQ010000414 DASRJQ010000000
VERSION     DASRJQ010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3488389"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VFB89_09285"
     CDS             complement(<1..212)
                     /locus_tag="VFB89_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27538.1"
                     /translation="MSQPAQQIRFCKSRDGTRIAYAAGGAGPPLVWAASWFHHLELDW
                     TSPIWRPWISLLTRRHTLVRYDWRGCG"
     gene            complement(259..660)
                     /locus_tag="VFB89_09290"
     CDS             complement(259..660)
                     /locus_tag="VFB89_09290"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZM27539.1"
                     /translation="MIEIKGVAHFSIAVSDLERSRRFYTEILGLKCVQDARGYGMVFL
                     RAGDDHVILAKSDAPLQRSAADSRRAHHAFKIDSTRYNEAKAFLTSRGVVVFEEEDRK
                     KGVFVGRQFYIHDPDANVIEFTEWNGTSVLG"
     gene            complement(683..1651)
                     /locus_tag="VFB89_09295"
     CDS             complement(683..1651)
                     /locus_tag="VFB89_09295"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HZM27540.1"
                     /translation="MKQSLAAMLSVSAMVAAGPAAAQTGYPERAIRLIVPFPAGGPSD
                     IIARLLGQRLADTWGKPLVIENVPGAAGNIGAERVAKASPDGYTLALVNIAQIVINPS
                     LYKLPYDPVKDFAPITQVVGSALVLVVHPSVPAKNVRDLVALARTRPGELTFASGGSG
                     NQPHLAGELLKSMTGIDITHIPYKGVPLAMPDLLAGRVTIMFGPIATVLPPLREAKLR
                     GLAVTGLQRSSAIPQLPTMDESGFRGFETTSWFGLLAPGGTPAPIVRKIHLETVRVLS
                     LPDVRAKISDLGMQVIGNTPDEFAAAIKAELPRWAKVIKETGIKAD"
     gene            complement(1796..>2853)
                     /locus_tag="VFB89_09300"
     CDS             complement(1796..>2853)
                     /locus_tag="VFB89_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZM27541.1"
                     /translation="RFCTSRDGTRIAYATCGAGPPLAWAAGWVHHLKLDWDSPVWRPW
                     ISLLARRHTLVRYDMRGTGLSDREGIEFSFEKFFEDFEAVTEAAGLERLVIYGAALGA
                     AIGMTYAVRHPERVSHLVLYSSYLRNRLAGSPTPQQVEEARARLKVIELGWPNDTPAY
                     GQFHGSLHMPDASAEQARSYNDLFRLTTSLANTVSMLQAVYRIDVREIVPRVRCPTLL
                     LHSRGNSIIPFEQGRSVAGLIPGARFVPLESRNNILLDTEPAWQQLVEALDEFLPASA
                     GTGDPLLDELTAREREVLELVAQGLDNGTIGTRLHISERTARNHVSAILAKLGMNTRA
                     QVIVRAREAGFGREKIR"
BASE COUNT          534 a          891 c          905 g          523 t
ORIGIN      
        1 ccgcagccgc gccaatcata acggacgagc gtgtgccgcc gcgtcaggag cgagatccag
       61 ggacgccaga tcgggcttgt ccaatcgagc tcgaggtggt ggaaccagct tgccgcccag
      121 acgagcggcg gaccagcacc cccggccgca tacgcgatac gcgtgccgtc gcggctcttg
      181 cagaagcgaa tctgctgcgc aggctgggac atgggacccc atcgggaggc tgcgcgcaat
      241 catagcgtat ggaccggatc aaccgagaac gctggtaccg ttccattccg tgaactcgat
      301 gacattagcg tcgggatcat ggatatagaa ctgccggccg acgaagaccc cttttttgcg
      361 gtcttcctcc tcgaaaacca ctacccccct ggaggtgagg aacgccttgg cttcgttgta
      421 cctggtcgag tctatcttga acgcatggtg cgcacgccgg ctgtcggcgg cgctgcgctg
      481 caacggggca tcgctctttg cgagaatgac gtggtcgtcg cccgcgcgca gaaacaccat
      541 gccgtagccg cgcgcgtcct gcacgcattt taacccgagg atctcggtat aaaacctgcg
      601 acttctctcg aggtcgctga ccgcaatgct gaaatgggcc acgcccttaa tctcgatcat
      661 tcctgcattc tccttggtga attcaatcag ccttaatgcc cgtctccttg atcactttgg
      721 cccacctcgg gagctcggcc ttgatggctg ccgcgaactc gtcgggagtg ttgccgatca
      781 cctgcatgcc gagatcactg atcttcgcgc gcacatcggg aagcgaaaga accctcactg
      841 tttcaaggtg aatcttgcgg actatgggtg ccggcgttcc gcccggggca agcaggccga
      901 accaggacgt cgtttcgaag ccccgaaatc cagactcgtc catggtgggt aactggggaa
      961 ttgcggacga gcgctgcagc ccggtcaccg cgagcccacg cagctttgct tcacgcaagg
     1021 gcggcaggac cgtggctatg ggcccgaaca ttatcgtgac gcgcccggcc agaaggtccg
     1081 gcattgccag gggtaccccc ttataaggaa tgtgcgtgat gtcgataccg gtcatggact
     1141 tcagcagctc ccccgccagg tggggctggt taccactccc gcctgacgca aaagtgagct
     1201 cgcccggtcg cgtcctggcg agggcaacca gatcccgcac gtttttcgcc ggcaccgacg
     1261 ggtgaacgac cagtaccagg gccgagccca ccacctgcgt gatcggtgcg aaatccttga
     1321 ccgggtcgta cggcaacttg tacagactgg gattgattac gatctgcgcg atattcacca
     1381 acgcgagcgt atacccgtcc ggggacgcct tggccacacg ttcggcaccg atgttgccgg
     1441 cagcaccggg gacgttctcg atgaccagcg gttttcccca cgtatctgca agcctctggc
     1501 cgaggaggcg cgcaatgatg tccgacgggc caccggcagg aaacggtacg atcagtcgga
     1561 ttgcccgttc gggatagcct gtctgagcgg ctgccgggcc agccgcaacc attgcggaga
     1621 cagaaagcat tgcagcgagt gactgtttca tgttccgcta ctcagtggat gacaggcccc
     1681 attgtgcaga aacggcggca aggggtcttg gggcagcggt actgtttttt gcaggcaaca
     1741 cgcccgggat ccggggcaga tgtcacgtcc ggagagccgg cccaccttct ccggatcagc
     1801 ggattttctc gcggccgaag ccggcctcgc gggcgcggac gatgacttgc gcccttgtgt
     1861 tcatgcccag cttcgcgagg atggcggaca cgtggttgcg ggccgtgcgc tcgctgatgt
     1921 gaagccgcgt cccgatcgtg ccgttgtcga gcccctgcgc caccagctcg aggacttcgc
     1981 gctcgcgcgc cgtgagttca tcgagcagcg ggtctccggt gccggcggac gcgggcaaaa
     2041 actcgtcaag cgcttccaca agctgctgcc atgccggctc ggtatccagg aggatgttgt
     2101 tgcggctttc aagcggaacg aaccgcgcgc caggtatgag cccagcgacg gaccggccct
     2161 gctcgaacgg gatgattgaa tttccgcggg agtggagcag gagcgtcgga cagcggactc
     2221 tcggcacgat ctcacgcacg tcgatccggt acaccgcctg caacattgag accgtgttgg
     2281 caagggaggt cgtcagacga aacaagtcgt tgtacgaccg ggcttgctcg gccgatgcgt
     2341 ccggcatatg caacgacccg tggaactgac cgtaagctgg ggtgtcgttg ggccaaccca
     2401 gttcgatcac cttgagcctg gctcgcgcct cctccacctg ctgaggcgtg ggactgcccg
     2461 ccaatctgtt gcgaaggtag ctcgagtaga gaacgagatg gctcactcgc tccggatggc
     2521 gtacagcgta agtcatgccg attgccgccc cgagcgctgc tccgtatatg acgagccgct
     2581 cgaggccggc ggcttcggtc accgcctcga aatcttcgaa gaacttttca aaagagaatt
     2641 cgatgccctc ccggtccgac aagccggtgc cgcgcatgtc gtagcggacg agcgtgtggc
     2701 gccgcgcgag gagagagatc cagggacgcc agaccgggct gtcccaatcc agcttgaggt
     2761 ggtggaccca acccgcggcc caggcgagcg gcggaccggc gccgcaggtc gcatacgcga
     2821 tgcgcgtgcc gtcgcggctc gtgcagaagc gga
//
LOCUS       DASRJQ010000415         6665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2543498, whole genome shotgun sequence.
ACCESSION   DASRJQ010000415 DASRJQ010000000
VERSION     DASRJQ010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6665
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2543498"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2567
                     /locus_tag="VFB89_09305"
     CDS             <1..2567
                     /locus_tag="VFB89_09305"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM27542.1"
                     /translation="ARVYLARDLRHDRPVALKVLHPELAHALGPERFLREIHTTARLD
                     HPHIISVFDSGETAGLLWYTMPYVEGESLRERLRREGQLPIEDALRIAREVADALSCA
                     HEQGIIHRDIKPENILLARGHARVADFGVAQALQQAGADRLTETGLAMGTPAYMSPEQ
                     AKGGSVDGRSDVYALGCVLYEMLAGEPPYTGPTSQAVITKRLSEPIPHLSIVRAVPPW
                     LEAAVTRALALSPADRFATAAEFTDALGVRSEPAAPTVPVRHRRRRWIAVLGGLAASL
                     AVLAGLWAAHRANRTGLDPKRVIVAVFANRTGNPTLDPMGLTAADYINRGLVQTGLVE
                     VVDVGVLYVQGRAATGEPSEPRALARRNGAGIVVAGSYDQFGDSLVFQAAIVDVGSGR
                     VVQALEPVRGPVARREMALDALRQRVTVGLAGLLDPRLSDLTTPSPEPPSFPSYQAFV
                     AGQSAYWGGSENEEALLQLRRAVRLDSTFLTAAVWVALVAADTPEPTGCAVADSVGRV
                     LLPHRDRLTLLDHLLLDAQLAACRGDNETASRLLSQAVPIPARSPQLQMFRALFTRQA
                     GRPREAVEILRRLDPERDLGWLPDSGKALYRRDLAVPYHTLGDYKSELRVARDLVRKD
                     PNRLAAINLESHALAGLGKTLEVLDLVERASRMPPDPLMAYALGKLTMGRMLLVNNAG
                     RLCYEAAVELQAHGHPEAAQKAAERAVNWYRAQPSVERSQVEYRYTLARSLELMGQYD
                     QADSLMKQLVAEAPTNVDYQGGLGVLAARRGDRTLAQHIDQMLGALSRPYLNGFPTYY
                     RAQIAAVLGDRSRAVELLRDAIAQGAVDPGDHLHSEPAFAGLHGYPPFEEVIRPKG"
     gene            complement(2592..3323)
                     /locus_tag="VFB89_09310"
     CDS             complement(2592..3323)
                     /locus_tag="VFB89_09310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HZM27543.1"
                     /translation="MGTHTAQRGGFTFHHRNEAEFELLFQEIFEEGEYRFASDRSDPT
                     ILDCGSHIGLSVAWFKRRFPQARIIAFEPDPENFRLLQTNIAINGFQGVELLNLAVSS
                     RRGTARFFGDFGVAAPMASAHSLRQEWGTQRAEQWILVNTVPLADYITGPIDYLKLDI
                     EGMEMEVMKSIEQYLHLVRAVGLEFHGTGGQARADEEDLLRLLRESGFQVSITNKSRS
                     IFPAEIDAWVNQVQPYVSMIKAARR"
     gene            complement(3585..4082)
                     /locus_tag="VFB89_09315"
     CDS             complement(3585..4082)
                     /locus_tag="VFB89_09315"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HZM27544.1"
                     /translation="MMQKIEIERFSLTSTKSFDEVLAAINDAIGHPEMAEFWKSIRQA
                     QTLAELESTIQKVLGSSGLMSFVQFDHGGIVQKATGRATRSVRLVVGNPLIMKELASR
                     VPDAGSYAPITILVDERADGVHLSYDRMASFLAPYNHPDALAVARTLDARVEDLLRRA
                     AGGGQ"
     gene            complement(4151..4348)
                     /locus_tag="VFB89_09320"
     CDS             complement(4151..4348)
                     /locus_tag="VFB89_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27545.1"
                     /translation="MRQTQDGIHDEMIPISNSYSLVENLPNVVLLAYPDSGHGSLFQF
                     HESFTQHAKTFLASNSPFAPY"
     gene            complement(4367..5200)
                     /locus_tag="VFB89_09325"
     CDS             complement(4367..5200)
                     /locus_tag="VFB89_09325"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZM27546.1"
                     /translation="MTESATPVRVIEETSAYWRVLFDYPPFNMVDATIFEGLQDLLAR
                     INASPSLRVVVFESANPEFYLAHFDLTGKTGNITTAVGASGLPILMDTFVRLTRSPVV
                     SIAKIRGCVRGVSSEFVLACDMRFASRENTRLGQPEVGVGVHPGGGGTERLPMLVGRG
                     RALEIVLGANDFDGDTAERYGYVNRALPDAELDGFVDLLARRIASFDRRAIAAAKNLV
                     NQVSLPSADRLLDALTSFQTALTWPETQQRIQALLKRGLQREADFENRWPAVLGTLLE
                     R"
     gene            complement(5312..5578)
                     /locus_tag="VFB89_09330"
     CDS             complement(5312..5578)
                     /locus_tag="VFB89_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27547.1"
                     /translation="MTLRIERTWAAGRVVVRLTGRMQAEFLPDLQKEIEETEAKPALD
                     LKEVTLVDVEAVQFLITCDNDGVSLLHCSPFIRQWMARERARRG"
     gene            complement(5608..6009)
                     /locus_tag="VFB89_09335"
     CDS             complement(5608..6009)
                     /locus_tag="VFB89_09335"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZM27548.1"
                     /translation="MNKTTPEQNKRLVLEAFDTLFNKRDYEAAKRYWSPDYIQHSAHI
                     EPGRDGLFNLVRSAPPTLRYEPGLIVAEGDHVIVHGRFSGRGSPANWIAADILRIENG
                     VLREHWDVLQDEATEEQSKSKVPMFGPSFPR"
     gene            complement(6035..>6665)
                     /locus_tag="VFB89_09340"
     CDS             complement(6035..>6665)
                     /locus_tag="VFB89_09340"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZM27549.1"
                     /translation="PTLQAEGHEVLSTQNCLDTVKGDVAAATRVLGRVSSPAILVGHS
                     YGGTVITAAGTDDRVAGLVYIAALAPDANETSQSLQAKFPTTDVFKHIEIADGRIWLR
                     PDGIECFAGDLSEAEQKLVWATQGVPATDLFDQKVEGTAWRSKPSWYVVARNDRTVHP
                     ELQRFAAKRMGASIHETDSSHVPMLSKPRYVLDVIRTAANAVLAGSTAA"
BASE COUNT         1180 a         2109 c         2101 g         1275 t
ORIGIN      
        1 tggccagggt ctacctcgcg cgggacctca ggcacgatcg gccggttgcg ctcaaggtgc
       61 tccaccccga gttggcccat gccctagggc ccgagcgctt tcttcgcgag atccacacga
      121 cggcccggct cgaccacccc cacatcatat ctgtattcga ttctggagag accgccgggc
      181 ttctctggta cacgatgccc tacgtcgagg gcgaatctct tcgcgagcgt ctgcggcgcg
      241 aaggccagct ccccatcgag gacgccttgc gcatcgcccg tgaggtggcc gacgcgctca
      301 gctgcgctca tgagcagggc atcatccacc gagacatcaa gcccgagaac attctgctcg
      361 cgcggggcca cgcgcgggtg gccgacttcg gcgtggctca ggcgctgcag caggccgggg
      421 ccgaccggct gaccgagact gggctggcga tgggcacccc cgcctacatg agtcccgaac
      481 aggcgaaggg aggctcggtg gatggccgca gcgacgtcta cgcgctcggc tgcgtgctct
      541 acgaaatgct cgccggcgag cctccctaca cgggacccac gtcgcaagcc gtcatcacaa
      601 agcgcctcag cgagccgatc ccccacctca gcattgtgcg cgccgtgccg ccgtggctgg
      661 aagccgcggt aacccgggca ctcgccctct cgcccgctga ccgcttcgcg actgccgccg
      721 agttcactga cgcattaggc gtgaggagtg aacctgccgc accgacggtg ccggtccgcc
      781 accgacgccg gaggtggatc gccgtcctcg gcggcctcgc cgccagcctt gctgtgcttg
      841 ccggcctgtg ggccgctcat cgggccaatc gcaccggctt agatccgaag cgagtgattg
      901 ttgcggtctt tgccaatcgg accgggaatc ccaccctcga cccgatgggg ctcaccgcag
      961 ccgactacat caaccgcggt ttggtgcaga ccggtctcgt ggaggtggtg gacgtcggag
     1021 tgctctatgt ccaggggcgt gccgcgactg gcgagccctc cgagccgcgt gccctggctc
     1081 ggcggaatgg cgcggggatc gtagtcgccg gaagttatga ccagttcggg gacagcctgg
     1141 tttttcaggc cgcgatcgtt gacgttggct ccgggcgtgt cgtccaagcg ctcgagccgg
     1201 tgcgggggcc ggtggcccgg cgcgaaatgg cgctcgatgc gctgcggcag cgggtgacag
     1261 taggcttggc cgggctgctc gacccgcgcc tgagcgacct caccacgccg tctcccgagc
     1321 cgcctagttt tccatcctac caggctttcg tcgccgggca gagcgcctac tgggggggca
     1381 gcgaaaatga agaggcattg ctgcagctcc gtcgggcggt gaggctggat tcaacgtttc
     1441 tcacggcggc agtctgggtg gcactggtag cggcagatac tcctgaaccg accggctgcg
     1501 cggtggccga ctccgtcgga cgcgtgctcc tcccccaccg cgatcggctc acgctccttg
     1561 accaccttct cctggacgcg cagctcgcgg cctgtcgagg ggataacgaa actgcatcac
     1621 ggctcctcag ccaggctgtc ccgatcccgg cgcgctctcc tcagcttcag atgttcaggg
     1681 cgctgttcac ccggcaggcc ggaaggcccc gcgaggctgt cgaaatcctg cggcgactcg
     1741 accccgagcg cgatctcggc tggctccccg acagtggaaa agcgctctac cggcgggacc
     1801 tcgcagtgcc ctaccatacg ctgggcgact acaaaagcga gctgcgggtc gcccgcgatc
     1861 tcgtgcggaa agacccgaac cgcctggcgg cgatcaacct cgaaagtcat gcgctggccg
     1921 gactcgggaa gactttggaa gtgctcgacc tcgttgagcg cgcgtcgaga atgcctcccg
     1981 acccactaat ggcgtacgca ctcggcaagc tcaccatggg ccggatgttg ctggtgaaca
     2041 acgccgggcg gttgtgctac gaggcggcgg ttgaactcca ggctcatgga catcctgagg
     2101 ccgcccaaaa ggcggcggag cgggccgtga actggtatcg cgcccagcca tccgtggagc
     2161 ggagccaagt cgagtaccgc tacactctcg cgcgctcgct ggagctgatg gggcagtacg
     2221 accaagcaga ttctttgatg aagcagctcg tggcggaggc tcccaccaac gtggactacc
     2281 aaggagggct cggcgtgctc gcagcccggc ggggcgaccg gacgctagcc cagcacatcg
     2341 atcagatgct cggcgcccta tcgcgaccat atctcaacgg cttccccacg tactatcgcg
     2401 cgcaaatcgc agccgtgctc ggcgaccgca gccgggcggt ggagctcctg cgggacgcca
     2461 tcgcgcaggg cgcagtagat ccaggagacc acctgcattc tgagcccgcc ttcgctggcc
     2521 tgcacggcta cccgccattc gaggaggtga tccggcccaa gggctgagat ggcaagagcc
     2581 gtaggccctc actacctgcg ggctgccttg atcatcgaga cgtaaggctg aacctggttc
     2641 acccaggcgt cgatttctgc cggaaagatt gatctcgact tattggtaat cgaaacctgg
     2701 aacccactct cgcgcagcag tcgtaggaga tcctcctcgt ctgcgcgggc ctggccgccg
     2761 gtcccatgaa attcgagacc aacagctcgg acaagatgca agtactgctc gatcgacttc
     2821 atcacttcca tctccattcc ctcgatgtcg agcttgaggt agtcgatcgg tccagtgatg
     2881 tagtcggcca aggggaccgt gttcacgagg atccattgtt cggctctctg ggtgccccat
     2941 tcctgccgca gcgaatgcgc agaagccatg ggagccgcaa ctccgaagtc tccaaagaac
     3001 cgagccgtgc ccctccggga cgagaccgcc aggttcaaca attcgacgcc ttggaatcca
     3061 ttgatcgcga tgttggtctg cagaagccgg aagttctcgg gatccggctc gaacgcaatg
     3121 attcgagcct gcgggaagcg tcgcttgaac caggccacgg aaagaccgat atgacttccg
     3181 cagtccagga tggtgggatc gctcctgtcg gatgcgaacc ggtattctcc ctcctcaaaa
     3241 atctcttgga agagtagctc aaattcggct tcgttccgat ggtggaaagt gaatccgccc
     3301 cgctgggccg tgtgagtccc cacagacgat tcctcgctgg cgatccggtg tcctgaggag
     3361 atggttctaa cggggcgaac gccgctgatc aacttgacga gaggctctcg cgtcgttagc
     3421 gccagttgag aaatgacttc cctattctcg ttgattgatg gcgagccatc caggtcagtt
     3481 cagcatctct ggtctcggct cctgcacccg ccgcgaggag tcgctggaag cgcgcattct
     3541 gccgaacggc atcgaaccgg cgctcgttgg tgagagtgcc ggagtcactg tcccccaccg
     3601 gcagctcgcc gtaagagatc ttccactctt gcgtccagag tcctcgcgac cgcaagcgca
     3661 tctgggtggt tatagggagc gagaaagcta gccattcggt catatgacaa gtggacgcca
     3721 tcagcgcgct catctaccag gatcgtaatg ggggcgtagg agccggcatc cggtacgcga
     3781 ctcgccagtt ccttcatgat gagcggattg ccaacaacga gacggacgct ccgagtagcg
     3841 cggccggttg ctttttgaac tatcccgcca tggtcaaact gcacaaaaga catcagtccg
     3901 gagctcccca aaaccttctg tattgtgctt tccagctcgg cgagcgtttg cgcctgccta
     3961 atcgatttcc agaattccgc catctccgga tggccaatgg cgtcattgat ggcggcgaga
     4021 acttcgtcga aggacttggt tgaggtcagg ctgaatcgct cgatctcgat cttttgcatc
     4081 atttgactga cctttgcttg tattgttctt gggctacggt ccatcccgat catccacagc
     4141 agccatcacc tcaatacgga gcgaagggcg agttggaagc gaggaacgtc tttgcgtgtt
     4201 gcgtgaatga ctcatggaac tggaatagtg agccgtggcc agagtccgga tacgccagca
     4261 agaccacgtt gggcagattt tcgaccagcg aataggagtt cgatatggga atcatctcgt
     4321 catgaatgcc gtcttgagtt tgccgcactg cgcaacgcca tggtgactac ctctctagaa
     4381 gagtaccgag cactgcgggc caccgattct cgaagtcggc ttcccgttgg agtccgcgct
     4441 ttaacagagc ttggattcgc tgctgcgttt caggccacgt cagcgcggtc tgaaaggagg
     4501 taagggcgtc gaggagccgg tcggcggatg gcaacgagac ctggttgacg agattcttcg
     4561 ccgccgcgat ggcacggcgg tcgaaggagg cgatccggcg tgcgagcagg tcgacgaagc
     4621 cgtccagctc cgcgtccgga agcgcccggt tgacatatcc gtatcgttcc gcggtgtccc
     4681 cgtcgaagtc gttcgcgccg agaacgattt cgagcgcgcg gccgcggccg accagcatgg
     4741 ggaggcgttc ggtaccgccg ccgccgggat gcactccgac cccgacctcg ggctggccca
     4801 gtcgcgtgtt ctcgcgcgac gcgaagcgca tgtcgcacgc gaggacgaac tcgctgctca
     4861 caccccggac gcagcctcgg atcttcgcga tgctgacgac tggggacctg gtgagacgga
     4921 cgaacgtatc catcaggatc gggaggccgg aagccccgac agctgtggtg atgttccccg
     4981 tcttccccgt caaatcgaag tgggcaaggt agaactcagg attcgcgctc tcgaagacta
     5041 cgacacgtag gctcgggctg gcgttgattc gagcgagcag atcctggagg ccttcgaaga
     5101 tggtggcgtc taccatgttg aaaggcggat aatcgaacaa gaccctccag tacgcagacg
     5161 tttcctcgat gacccgcacg ggcgtcgctg attcagtcat gattcacgcg ccttgcgacc
     5221 acaatcgagc gcgacgacaa ggcgctgttt ttctcttccg tggtggtgat cggtggtcgg
     5281 tttggtcata ggctcctttc tccttccatc attatcctcg acgcgcgcgt tccctggcca
     5341 tccactgccg gataaacggc gagcagtgca gcagcgagac gccgtcgttg tcgcaggtga
     5401 tgagaaactg cactgcttca acgtcgacga gcgtgacttc cttgaggtcc agagccggct
     5461 ttgcctcggt ctcctcgatc tccttctgaa ggtcggggag aaactccgcc tgcattcggc
     5521 cagtcagccg aacgaccact cgcccggccg cccaggttct ctcgatgcgc agcgtcacgg
     5581 tcgccccctg agccctcctc ccccggctta tctcgggaag cttggcccga acatcggcac
     5641 tttgctcttg gactgttcct cggtggcttc gtcttgaagc acgtcccagt gttctcgcag
     5701 aaccccgttt tcgatgcgaa ggatgtcggc agcaatccag ttcgcgggcg agccgcgacc
     5761 gctgaatcgc ccgtgaacga tgacatgatc gccttcggcc acgatcagac cgggctcgta
     5821 ccgcaacgtg ggaggagcgc tccggaccag attgaacaag ccgtcacggc ctggttcgat
     5881 atgagcgctg tgctggatgt agtccggaga ccagtagcgc ttcgctgctt cgtagtcgcg
     5941 cttgttgaac aacgtgtcga aggcttccag gacgagtctc ttgttctgtt caggtgtcgt
     6001 cttgttcaac gggcctcctc agtggccacg cgatttacgc agccgtggat cctgctagaa
     6061 ccgcgttcgc ggccgtacgg atcacgtcga gcacgtacct gggtttggag agcatcggga
     6121 cgtggctgct gtcggtctca tggatagagg cgcccatgcg cttggccgcg aagcgctgca
     6181 actcggggtg gacggtgcgg tcattcctgg ccacgacgta ccagctcggc ttcgaccgcc
     6241 aggcggtgcc ttccaccttc tggtcgaaca ggtccgtggc cggcacgccc tgggtcgccc
     6301 agacgagctt ctgctccgcc tcggacaggt ctcccgcgaa gcactcgatg ccgtcgggcc
     6361 gcaaccagat gcgcccgtcg gcgatttcga tgtgtttgaa gacgtcggtg gtggggaact
     6421 tggcctgcag gctctgcgag gtctcgttgg cgtccggggc aagtgcggcg atgtagacca
     6481 gcccggccac gcggtcgtcg gtgccggcgg cggtgatgac ggtgccgccg tacgagtggc
     6541 cgacgagaat ggccgggctg ctgacccgtc ccagcacccg ggtggcggcg gcgacgtcgc
     6601 ccttgacggt gtcgaggcag ttctgggtgg acagcacctc gtgcccctct gcctgcagcg
     6661 taggg
//
LOCUS       DASRJQ010000416         2058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1050230, whole genome shotgun sequence.
ACCESSION   DASRJQ010000416 DASRJQ010000000
VERSION     DASRJQ010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050230"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VFB89_09345"
     CDS             complement(<1..555)
                     /locus_tag="VFB89_09345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HZM27550.1"
                     /translation="MKAIVVTDEAAGTAGMTLVERAEPPVAINDVVVRIHASGFVPTE
                     MAWPSTWTDRRDRDRTPSIPGHELAGVVTALGYGTTGVSVGQRVFGLADWHRDGTLAE
                     YVAIEARNLAPLPGDVDFTVGASLPISGLTAWQGLFEHGRLQAGQSVLVHGAAGAVGS
                     MVTQLAREFGAYVIGTGRAGDRQKT"
     gene            860..1276
                     /locus_tag="VFB89_09350"
     CDS             860..1276
                     /locus_tag="VFB89_09350"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZM27551.1"
                     /translation="MPIHHVDLAVADVERSLAFYRALLGPLGWTKEVRYPTYRGTEEV
                     VYLQEPTTRSGLGLRPADEGEYRYYAVGIEHLAFEVDERDEVDAAYTRCLARDANIHF
                     PPEEDRDIEGYYAFFVFDPDGIRVEVLWWPRDEPAG"
     gene            complement(1297..1698)
                     /locus_tag="VFB89_09355"
     CDS             complement(1297..1698)
                     /locus_tag="VFB89_09355"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZM27552.1"
                     /translation="MSEENVEIVRHLYESGLFDRDPEELLKLATPDIEYVNPPYAVEP
                     GVRHGLVAVAQAMRRFAEVWDTSRHELRELYDCGDAVVAAVNWHIRGRGSEQEFVNKE
                     AHTWTLRAGRIARFEWGQDLGTALEAAGFPH"
BASE COUNT          354 a          703 c          648 g          353 t
ORIGIN      
        1 cgtcttctga cggtcgccgg cgcgtccggt gccgatgacg taggcgccga actcgcgtgc
       61 gagttgggtc accatcgacc cgactgcacc ggccgcgccg tgcacgagga cactctgccc
      121 cgcctgaaga cggccgtgct cgaacagtcc ctgccatgcg gtcagacccg agatcggcag
      181 gctcgcgccc accgtgaagt cgacgtcgcc cggcagcggc gcgagattgc gtgcctcgat
      241 cgccacatac tccgccaggg tgccgtcgcg atgccagtcc gcgaggccga acacccgctg
      301 tcccaccgat acccccgtcg tgccgtagcc gagagcggtg accactccgg ccagctcgtg
      361 cccagggatc gacggtgttc ggtcacggtc gcggcgatcg gtccaggtcg agggccacgc
      421 catctcagtc ggcacgaatc ccgacgcatg aatccgaacg acgacgtcgt ttatcgcgac
      481 cggcggctcg gcccgctcca ccagcgtcat cccagccgtt cccgcagctt cgtccgtcac
      541 cacaatcgcc ttcatccggc acctacttgg ttcatctact tgccgctctc gtcaatgtgc
      601 ccagggcggg gcacacccat gccggagacg atgcccaagc cggattccgg gcatgttccg
      661 atcgctccaa ggaaccaacc ggactgagcg aacggctcac tgggagcgag tggtaagcga
      721 accgttcccg agtggaagtc gctgctgctc ttcgtccaaa gcggcagtct cgcctcctcc
      781 cgcagcccgg cggcttcgag ggcctgggcg ctggccgata gggtcggacg aacgcaaccc
      841 tgggggactc gtgccagcaa tgcccatcca ccacgtcgat ctcgctgtcg ccgatgtcga
      901 gcgatccctc gcgttctacc gcgcccttct tgggccgctc ggttggacaa aggaggtccg
      961 atacccgaca tatcgaggaa ccgaggaagt cgtgtacctg caggaaccga cgacccgttc
     1021 ggggcttggg ctgcgaccgg ccgacgaagg tgagtaccgc tactacgccg tgggaatcga
     1081 gcacctggcg ttcgaggtcg atgagcggga tgaggtcgat gccgcgtaca cacgatgcct
     1141 cgcacgcgac gcgaacattc acttccctcc cgaggaagat cgcgacatcg agggctatta
     1201 cgcattcttc gtcttcgatc cggacgggat tcgcgttgaa gtcttgtggt ggccgcgtga
     1261 cgagccggcg ggctagccgg aactaaggcg agcggtctaa tgcgggaagc ccgcggcctc
     1321 gagagccgtc ccgagatcct gcccccattc gaaccgagcg atcctgcccg cgcgcagggt
     1381 ccaggtgtgg gcctctttgt tgacgaactc ctgctcgctt ccgcggccgc gtatgtgcca
     1441 gttgacggcg gccacaacgg catctccaca gtcgtacagc tcccggagct cgtggcggga
     1501 tgtgtcccag acttcggcga agcggcgcat cgcctgcgcg acggcgacga gcccgtggcg
     1561 cacacccggt tcgaccgcat agggagggtt gacgtactcg atatcaggag tcgcgagctt
     1621 caggagctcc tcaggatctc gatcgaacag ccccgactcg tagaggtggc ggacgatctc
     1681 gacgttctcc tccgacatcg ccgagtagtc tctcgcgaac gccgctttcc atgcaatgcg
     1741 gtagccacga cgatcgagcg aggctcaggt cttcgacagg agctctctca ggtcgatcga
     1801 aaggtttgaa agctgtccgg ctagctccat tcgagttcac cgcgactact cggcgccctc
     1861 accaggtcgt tctgggaggc gcccggcgcc gattccacct ggcagcccgg cgtgtgtctg
     1921 tgacgggctg cggtgtcagc ggtgcggcca gccccccggg agaacgccag gtacccgtat
     1981 ttccaccgaa ctgcggccgc tggattctcc tcgcacaggc tgaccgcacc agacgccggg
     2041 agagcaggcc agatgagc
//
LOCUS       DASRJQ010000417         6535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_186281, whole genome shotgun sequence.
ACCESSION   DASRJQ010000417 DASRJQ010000000
VERSION     DASRJQ010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6535
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186281"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VFB89_09360"
     CDS             complement(<1..261)
                     /locus_tag="VFB89_09360"
                     /inference="COORDINATES: protein motif:HMM:NF013734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27553.1"
                     /translation="MNASRRWLEDFLKRPLETRELVGKLVMLGAPVDAVEALHAGLGE
                     IRVALVESVAPHPNADRLRVCEVNDGSATVKHVVCGAPNVTAG"
     gene            complement(258..1355)
                     /gene="pheS"
                     /locus_tag="VFB89_09365"
     CDS             complement(258..1355)
                     /gene="pheS"
                     /locus_tag="VFB89_09365"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HZM27554.1"
                     /translation="MADPTGSSELDALRARVERIPSLDAAQLEAEHIALLGRRQGTIT
                     ELLKRLPELPLEQRKDFGAKVNQLKVRADALIAERRAALETERRGRSDRVDLTMPPRR
                     RWVGAEHPVTRVIDELLGIFRELGFAVAVGPEVETEWLNFGALNFPPDHPAMDLHDTI
                     YLDAPPIEEKPHGGRLLLRTHTSPVQIRVMLASKPPIRVVIPGMCYRNDPFDPSHAPA
                     FQQIEGLAVDEGISFVDLKATLTQFARRFFGPATKVRFRPSFFPFTEPSAEMDVECQL
                     CHGSGCPACKGTGWMEILGSGMVHPHVIENGGLDPEQYTGFAWGMGPHRIALLRYGVP
                     DIRLFYEGDMRFLGQFVPGRAAGWAGDRTPA"
     gene            complement(1466..1819)
                     /gene="rplT"
                     /locus_tag="VFB89_09370"
     CDS             complement(1466..1819)
                     /gene="rplT"
                     /locus_tag="VFB89_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HZM27555.1"
                     /translation="MPRVKSGVAHHARKKKYMEAAKGARGGRSKLYKSAKETVERGMR
                     YAYRDRRKKKAEFRALWITRINAAARLHGLSYSRLMAGLKKAGVEVNRKLLADLAVRD
                     ADAFSRIAEVAKGQS"
     gene            complement(1830..2024)
                     /gene="rpmI"
                     /locus_tag="VFB89_09375"
     CDS             complement(1830..2024)
                     /gene="rpmI"
                     /locus_tag="VFB89_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HZM27556.1"
                     /translation="MPKQKTKRAAMKRFTRTGSGKLKRFHANHTHILTKKTRKRKNRL
                     KKGALVSSADFPRVNRLLQA"
     gene            complement(2049..2612)
                     /gene="infC"
                     /locus_tag="VFB89_09380"
     CDS             complement(2049..2612)
                     /gene="infC"
                     /locus_tag="VFB89_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HZM27557.1"
                     /translation="MGVPFFILRKEVRPLSTNEREKRVRVNRQIRISPVRVIGPGGEQ
                     LGVMPVDEALAAAQDRGLDLVEVAPMARPPVVKIMDYGKFKFEEAKAARAAKKKQHII
                     QLKEVKYRPGIDDHDFDFKTRHAREFLGDGNKVKVTMMYRGRQIAHLELGKAVLDRVA
                     AALADVGKVESEAKLEGRNMTMVIAPK"
     gene            complement(2770..3690)
                     /locus_tag="VFB89_09385"
     CDS             complement(2770..3690)
                     /locus_tag="VFB89_09385"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZM27558.1"
                     /translation="MTLVHTFTIGRFRCHTIEAGLQRLDGGAMFGVVPKPMWEKRMPP
                     DARNRIPLALRCLLVEHPDGLVLVDTGVGDKDDARFRDIYGIENQGRNGSTRLDDGLA
                     ELGFSADDIKLVVNTHLHFDHAGGNTRLAGASGPLLDTGEFGAEPAFPNAVYVVQRGE
                     LEFARHTNERTRASYIPRNFEPISVANRWRLFNGDGDVVPGVAVRLTPGHVPHHQSII
                     IRDAGETAVFLADLIPTAAHMPLPWIMGYDLEPLRTLESKRAVLREAVAGDWLLIFEH
                     DPQIATARLEEGAKALDLRDVTPATPVEAR"
     gene            complement(3687..4562)
                     /locus_tag="VFB89_09390"
     CDS             complement(3687..4562)
                     /locus_tag="VFB89_09390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HZM27559.1"
                     /translation="MIGLVAHPSRTLSDRALDQLSSGPATSAVLCDLVLGLAGAPAVI
                     AERLAAALLGPDPRVCQLADGRWSLVAEAFGSPLLEECAFAVVDVETTGSSAGGGDRI
                     TEIAVVVVQGTRCEVVFETLVNPGRPIPMPTSMVTGITDAMVRAAPTFAEVADGLLTA
                     LAGRVFVAHNARFDWTFLTAELKRVRDLGLNGPRLCTVRLSRRLLPGLRSCGLDSVSN
                     WFGFENPARHRAGGDALVTAKLLARLLGLAREAGARTLADLASLEARRARKAKRKRSA
                     MPSEPRADSYPGYDA"
     gene            complement(4559..5791)
                     /locus_tag="VFB89_09395"
     CDS             complement(4559..5791)
                     /locus_tag="VFB89_09395"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HZM27560.1"
                     /translation="MSCLHVSSEIGALQSVLVHTPGPELHAVTPGTREDYLYDDIIDL
                     ETAQREHRQFVAVLSRFAQVMQVRKLVAEVLDNPEARNFVITKTMDVVPSDALAQQLT
                     ALPADEIVRLLVEGTEEPSGPIARALNEVGFALPPLPNLFFPRDIGIVIGEHVVVGAM
                     RYGVRWTEELLIKALFCFHPALANAGLLYDGSDERRVNYTLEGGDVHQLRPDLVVVGF
                     SERTSPAALDQLTDLVFSRGKITDVVVVVIPKSPTAIHLDMVFTQVDRDLCVVYPPSF
                     VGPERVPILHRRKGEAGVREMPNLFAALRAVDLPLEPIFAGGDHRTTQDREQWSSACN
                     FVALRPGTIVSYRRNYATLEALKEAGFRVVPSSDFLAFDDWAEARHRIVITVDGSELA
                     RGGGGPRCMTLPLRRAAL"
     gene            complement(5891..6511)
                     /locus_tag="VFB89_09400"
     CDS             complement(5891..6511)
                     /locus_tag="VFB89_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HZM27561.1"
                     /translation="MRKVAESTVRRLSLYLRFLEEFEEQGIDTISSEALASRGGTTSA
                     QVRKDLSFFGSFGKRGLGYAVPELAGRIREILGLERRYRVAMIGAGKIGSALVQYRGF
                     RQRGFDIVAIFDVDPTKVGHQWNGLTVQDIGHLEVELGKHPIDMAVLVTPADAAQPVA
                     DRLVKLGVKAILNFAPLQLTVPEDVVVKSVNLALELETLSYALSNR"
BASE COUNT         1159 a         2185 c         2051 g         1140 t
ORIGIN      
        1 ccccgccgtg acattcggcg cgccgcagac cacgtgcttg accgtggcgg agccgtcgtt
       61 cacttcgcaa acgcgcaacc ggtctgcatt cgggtgcggt gccaccgact ccaccaaagc
      121 cacgcggatc tctcccagcc cggcgtgaag cgcctcgacc gcatccaccg gtgcgcccag
      181 catcacgagt ttgcccacca gctcacgcgt ttcgagcgga cgctttagga aatcttccaa
      241 ccaccgacga gacgcgttca tgccggcgtc cgatctccgg cccatccagc ggcccgcccg
      301 gggacgaact ggccgaggaa ccgcatgtcg ccttcataga agagccgaat gtccggcacg
      361 ccgtagcgga gcagtgcgat gcggtgggga cccatgcccc acgcgaaacc ggtatactgt
      421 tccggatcga gcccgccgtt ctcgatcacg tgcgggtgta ccatgccgga gcccagaatt
      481 tccatccatc cggttccctt gcaggccgga cagccgctgc cgtggcacag ctggcactcg
      541 acgtccatct cggcagaggg ctcagtgaac gggaaaaacg acgggcgaaa ccgcaccttt
      601 gtcgctggac cgaagaaccg ccgcgcgaat tgagtcagcg tggccttgag gtctacgaag
      661 gagatcccct cgtccaccgc gagcccctcg atctgctgga aggcaggagc gtgcgacgga
      721 tcgaagggat cgttgcggta gcacatgccc ggaatgacca cgcggatggg cggcttggaa
      781 gcgagcatga ccctgatctg caccggcgat gtgtgagtcc ggagcagcag gcgcccgcca
      841 tgtggcttct cctcgatcgg cggcgcgtcg agatagatcg tgtcgtgcag gtccatcgcc
      901 ggatggtccg gcgggaagtt gagcgcaccg aagttgagcc actcggtttc cacttcggga
      961 ccgacggcaa cggcgaatcc cagctcacgg aaaattccca ggagctcgtc gatcacccgg
     1021 gtgaccggat gctcggcccc tacccagcga cggcgaggcg gcatggtgag gtccacccgg
     1081 tcggaccggc ctcgccgctc cgtttccagc gcagctcgcc gttcggcaat cagtgcgtcg
     1141 gcccggacct tgagttggtt gaccttggcg ccgaagtcct tccgttgctc gagcggaagc
     1201 tccggaagac gcttaagcag ctcggttatg gtaccctgcc tccgacccaa tagcgcaatg
     1261 tgctccgcct cgagttgggc cgcatcgaga gacggaatgc gctccactcg cgccctcagg
     1321 gcatcgagct cggaagatcc ggtggggtca gccattcatg atccggagaa gaaacagtgc
     1381 atgcgaccgt ggggccatcc gcgccgggcg gacggcccca cgggcggagg ctcgcgacgg
     1441 gtgcggtgga gggacaacgg gtcccttacg actgcccctt cgcgacctcc gcaattcggg
     1501 aaaaggcgtc cgcgtcacgc acggcgaggt ccgcgaggag cttgcggttc acctcgacgc
     1561 cggccttctt gagtccggcc atcaagcggc tgtagctcaa gccgtgcagt cgtgccgcgg
     1621 cgttgatgcg cgtgatccag agcgcccgga actctgcctt cttcttgcgc cggtcccgat
     1681 aggcgtaccg catcccccgc tccaccgtct ccttcgccga cttgtacagc ttgctccggc
     1741 cgccgcgcgc acccttcgcg gcttccatgt acttcttctt gcgggcgtgg tgcgcgacgc
     1801 cgctcttcac gcgaggcata gggacgtcct caggcctgga ggaggcggtt cacgcgcggg
     1861 aagtcagccg agctcaccag ggcgcccttc ttgagccggt tcttccgctt gcgcgtcttc
     1921 ttggtcagga tgtgcgtgtg attcgcgtga aaccgcttca gcttccccga tccggtccgg
     1981 gtgaagcgct tcatcgcggc gcgtttggtc ttctgcttcg gcatcgtgct ctcgttgtcg
     2041 ggcgccgttt atttcggcgc gatcaccatc gtcatgttcc ggccctcgag cttggcctcg
     2101 gactcgacct tccccacgtc cgcgagcgcc gccgcgaccc gatccagcac cgccttgccc
     2161 aattcgaggt gggcgatctg ccggccgcgg tacatcatcg tcaccttcac cttgttgccg
     2221 tcgcccagga actcgcgcgc gtgacgcgtc ttgaagtcga aatcgtgatc gtcgattccc
     2281 ggccggtact tcacttcctt gagctgaatg atgtgctgct tcttcttggc ggcccgtgcc
     2341 gccttcgctt cttcgaactt gaacttgccg tagtccatga tcttgaccac gggcgggcgc
     2401 gccatcggcg ccacttccac gagatccaac ccacggtcct gcgctgctgc cagcgcttcg
     2461 tctacgggca tcacccccag ttgctcgcct ccgggaccaa tcacccgcac cggactgatg
     2521 cggatctgcc ggttgacgcg gactcgcttt tcgcgctcgt ttgtggacag tggccgaacc
     2581 tccttccgca aaatgaaaaa agggacaccc aagtaccgga tgtcccagca ctgcggccga
     2641 gcgcctggtg gcgatgcagc cggcagaggc cttggacccg gtcacaccgc gcgactctgc
     2701 gggctcaggg tgggggaagt ccgttccccg cttgcaaggc gcgaattgta ccccccgaag
     2761 caaagggtgt caacgcgcct cgaccggcgt cgctggagtg acgtcacgca gatcgagtgc
     2821 cttggcgccc tcctccaggc gtgcagtcgc gatctgcgga tcgtgctcga aaatgaggag
     2881 ccaatccccc gccactgcct cgcgtaacac cgcccgcttg ctctccagcg tgcgaagcgg
     2941 ctccaggtca tatcccatga tccaagggag cggcatgtgg gccgcagtcg ggatcagatc
     3001 ggccaggaat accgccgtct cgccggcatc gcggatgatg atgctctgat ggtgcggcac
     3061 gtgcccgggc gtgagccgaa ctgcgacccc cggcacgaca tcgccgtcgc cgttgaacag
     3121 cctccaccga tttgccaccg atatcggctc gaagttgcgc gggatgtagc tcgcgcgggt
     3181 ccgttcgttg gtgtgccggg caaattcaag ctctccccgc tgcaccacat acaccgcgtt
     3241 gggaaacgcc ggctccgccc caaattcccc ggtgtcgagc agcgggcccg acgcgccggc
     3301 gagccgggtg ttgccgccgg cgtgatcgaa atgcaggtgg gtgttcacca cgagcttgat
     3361 gtcgtcggcc gaaaagccca gctcggcgag cccgtcgtcc agccgggtac tgccgttacg
     3421 accctggttc tcgatgccgt aaatgtcgcg gaacctggcg tcgtctttat cgcctacgcc
     3481 ggtatcgacc agaaccaatc cgtccggatg ctccaccagc aggcagcgca gggcaagtgg
     3541 gatgcggttg cgtgcgtcgg gcggcatccg tttttcccac atcggcttgg gcaccacgcc
     3601 gaacatcgcg ccgccgtcga ggcgctgaag gccggcttcg atcgtatggc agcgaaaacg
     3661 tccgatagta aaggtgtgca ccaacgtcat gcgtcatatc cagggtacga gtctgcgcgg
     3721 ggctcgcttg gcatcgcgga gcgcttgcgc tttgccttcc gggcccggcg cgcctcgagt
     3781 gaggcgagat cggcgagcgt acgggcgccg gcttcgcgtg ccaaccccag aagcctcgcc
     3841 aggagtttgg ccgtaacaag cgcgtcgcca ccggcccgat gccgggccgg attttcgaat
     3901 ccgaaccagt tgctcaccga gtcgagaccg caggagcgca agccgggcag gaggcgccgc
     3961 gacaggcgca cggtgcagag ccggggtccg ttgagcccga ggtctcggac tcgcttgagc
     4021 tcagccgtga gaaaggtcca gtcgaagcgg gcattgtggg ccacgaagac gcggccggcg
     4081 agtgccgtca gcaagccgtc ggcaacttca gcgaacgtcg gcgccgcgcg caccatcgca
     4141 tcggtaatcc cggtgaccat gcttgtgggc atcggtatgg gtcgtccggg attgaccagc
     4201 gtctcgaaca cgacttcgca acgtgtacct tgaacgacca ccacggcaat ctcggttatc
     4261 cgatcgcccc cgccggcgct cgatccggtc gtctcaacgt ctaccaccgc aaaggcacac
     4321 tcctcgagca gcggcgagcc gaaggcttcc gccaccagag accagcggcc atcagccagt
     4381 tggcaaaccc gcgggtcggg gccgagcagg gcagccgcga ggcgctcggc gattaccgcg
     4441 ggggcgccgg cgaggccgag cacgaggtcg caaagcacgg ccgacgtcgc cggacctgag
     4501 gacaactggt cgagtgctcg atcagacagg gttcgactgg gatgggcgac gaggccgatc
     4561 acagcgcggc gcggcgcaga ggaagcgtca tgcagcgcgg gccgccaccc ccccgcgcca
     4621 gctcacttcc atcgacggtg atcacgatcc gatgccgggc ttcggcccaa tcatcgaaag
     4681 ccaggaaatc gctcgatggt accacccgga agcccgcttc cttgagcgcc tccagcgttg
     4741 cgtagttccg ccgatagctc acgatcgtgc ccggtcggag cgcgacgaag ttgcacgcgg
     4801 agctccactg ctcccggtcc tgagtggtgc gatggtcgcc gccggcaaag atcggctcga
     4861 gcggaagatc gactgccctg agcgcggcga aaaggttggg catctcacgc acacctgcct
     4921 cgccctttcg ccggtgcagg atggggaccc gctcggggcc aacgaaggat ggcgggtaga
     4981 ccacgcagag atcgcgatcc acctgggtga acaccatgtc gagatggatc gcagtgggcg
     5041 acttgggaat gaccacgacc accacgtcgg tgatcttgcc gcgggaaaac accagatcag
     5101 tcagttggtc cagcgcggct ggggaggtgc gctcgctgaa tccgaccacc acgagatcgg
     5161 gtcgcagctg gtgaacgtcg ccgccttcca gcgtgtagtt gacccggcgc tcatccgagc
     5221 cgtcgtaaag cagcccggca tttgccaggg ccggatggaa gcagaacagc gccttgatca
     5281 gcagctcttc ggtccagcgc acaccataac gcatggcgcc caccactacg tgctcgccga
     5341 tcacgattcc gatatcgcgg ggaaagaaca gattgggcag cggcggcaga gcaaagccca
     5401 cctcgttcag cgcccgtgcg atcggcccgc tcggctcttc agttccctcc accagcaagc
     5461 gcacgatctc gtctgccggg agggccgtga gctgctgcgc gagcgcgtcc gaaggcacca
     5521 catccatggt cttggtgatg acgaaattcc gcgcctccgg attatccagc acttcggcca
     5581 ccagcttgcg cacctgcatg acctgggcga aacgggaaag cacggcgaca aactgtcggt
     5641 gttcccgctg cgcggtctcg agatcgatga tgtcgtcgta gagatagtct tcgcgggttc
     5701 cgggcgtcac cgcgtgcagc tcgggtccgg gcgtgtggac caggaccgat tgcaaagcac
     5761 caatttcgga ggagacgtgg aggcaggaca tgtccagcaa cgtaaacggt gaacggtgaa
     5821 cggtgaacgg gggggacccg gcggcgaggc cggtcccgtt taccgtttac cgttcaccgt
     5881 ttacgcagct ctatcggtta gacaacgcat agctcaacgt ctccagctcc agcgccagat
     5941 tcacgctctt caccaccaca tcttcaggca ccgttaactg gagtggagcg aaattgagaa
     6001 tcgccttgac cccaagcttc accaggcgat cagccaccgg ctgcgcggca tccgccgggg
     6061 taaccagcac cgccatgtcg atgggatgct taccgagctc cacctcgaga tgcccgatgt
     6121 cttgtaccgt aagcccgttc cattgatgac ccaccttggt ggggtccacg tcgaagatgg
     6181 cgacgatgtc gaacccgcgc tggcgaaagc cccgatactg gaccagcgcg cttccgatct
     6241 tccccgcccc gatcatcgcg acccgatagc gccgctcaag ccccagaatc tcgcggatcc
     6301 gaccggccag ctccggcacg gcgtagccta gcccgcgctt accgaaggag ccaaagaacg
     6361 aaaggtcctt ccgcacctgg gccgaagtcg tcccaccccg ggaggccagt gcttccgagc
     6421 tgatcgtgtc gatgccctgc tcttcgaact cttccaggaa tcgcaggtac agcgagaggc
     6481 ggcgaaccgt gctctcggcc actttgcgca tgggccgctt ttgtgaaatg tttca
//
LOCUS       DASRJQ010000418         3798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3443192, whole genome shotgun sequence.
ACCESSION   DASRJQ010000418 DASRJQ010000000
VERSION     DASRJQ010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3443192"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="VFB89_09405"
     CDS             <1..1006
                     /locus_tag="VFB89_09405"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HZM27562.1"
                     /translation="AAITGTLARLSRRGAAAVLLVASAPVDSAYDPVAVQRQRGAYDV
                     DHATPRLAGQPDRVAPPPAAGTGPTPAFLVRAGMAATLHAGPEATLAIRLERFETPSV
                     NVIGALRGTDPALRDEYVLYSSHQDANGVRLLAAGDSVLAGADDNASVSVAQFAAARA
                     FVAQRPKRSLLFVHHGAEERGLLGSRYHSAHPVVPLGQIVTVLNGDMIGRNHPDTATL
                     LGAQPPHRNSSELVAMALQANAATGRFVVDTLWDRPTHTEGWYFRSDHLPYARLGIPA
                     LMYSTNLHDDYHTAGDLPERIDYAKLTRMARWMYLTGWYVANAPRRPALDAGFKLERE
                     "
     gene            1006..1458
                     /locus_tag="VFB89_09410"
     CDS             1006..1458
                     /locus_tag="VFB89_09410"
                     /inference="COORDINATES: protein motif:HMM:NF019238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HZM27563.1"
                     /translation="MRNISDGVVLEDLVRRLRRLAPTLPGLWGTMKAQQMALHVGDAC
                     AAVLKQRDFSAKPRSGPAGLLRIIVLYLLPRMPRGVKSGANPAAAVVEPEAFARDLER
                     AVTLLQQLATEAPDGLVDRHPIFGPMTRADWMRWAFLHTDHHLRQFGL"
     gene            1511..1879
                     /locus_tag="VFB89_09415"
     CDS             1511..1879
                     /locus_tag="VFB89_09415"
                     /inference="COORDINATES: protein motif:HMM:NF040323.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZM27564.1"
                     /translation="MLRLAIPVLHVSSSMAAERFYCVGLGFLQRFAYRPDDGHADPCY
                     MGLIRDGVLLHLSSFPGDGVAGGVANFIVDDLDALHEEFRRKGLRIELEPTDQSWGNR
                     EMYLEDADGNSIRFVLNTGA"
     gene            1947..2381
                     /locus_tag="VFB89_09420"
     CDS             1947..2381
                     /locus_tag="VFB89_09420"
                     /inference="COORDINATES: protein motif:HMM:NF021612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase-like protein"
                     /protein_id="HZM27565.1"
                     /translation="MSANTTVVLAALAAVILIAATCYLAFQLRFERWKRDHTKAARRD
                     AVQRSQAVTLGKIYEHLIPYLPDFRWNPKDARFIGTPVDFLVFDGLSEGQVRAIIFVE
                     VKTGTSSMSVRERQVRDAVLGQRVEWHQLNLPDVRMRPDPAA"
     gene            2389..2949
                     /locus_tag="VFB89_09425"
     CDS             2389..2949
                     /locus_tag="VFB89_09425"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HZM27566.1"
                     /translation="MKTFRAQTLDQWRDWLAAHHDSESEVWLIFHKVHTGVPSIAYQD
                     ALDEALCFGWVDSLVKRLDERRYARKFTPRTANSRWSAINRTRYAALKASGRLRAAGL
                     RRAPTERGHDPRPSRFTMPSKLPPYIAAALRKRPAAMRHFEALAPSLRRNYVGWIESA
                     KRDETKARRLKEAIRLLAAGKELGLK"
     gene            2946..3581
                     /locus_tag="VFB89_09430"
     CDS             2946..3581
                     /locus_tag="VFB89_09430"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZM27567.1"
                     /translation="MSLPAGLQEQFGEIDIYLFDQLLRGRIVPGMRILDAGCGSGRNL
                     VYLLQAGYEVFGADEDAASIAEVRRLAARFAPALPADNFRVESLEALTFPSLFADTVI
                     CSAVLHFAADDARFNAMLQGCWRVLKPNGLFFCRLASSIGLDGRGEPLGGRRYRLPDR
                     SERYLVDEPLLLALTRGLGGELVDPLKTTVVQDQRCMTTWVVRKALSDGAP"
BASE COUNT          645 a         1286 c         1199 g          668 t
ORIGIN      
        1 tgccgccatt accggcacgc tcgcccgcct gtcccggcgc ggcgccgccg cggtgctgct
       61 ggtggccagc gcaccggtgg acagcgccta cgatcccgta gcggtccagc ggcaacgggg
      121 ggcgtacgac gtggaccatg cgaccccgcg gcttgccggt cagcccgacc gggtggcacc
      181 acccccggcg gccggtaccg gcccgacgcc cgccttcctg gtgcgtgccg gaatggccgc
      241 gaccttgcac gcgggaccgg aggccacgct cgccattcga ctggaacgct tcgagacgcc
      301 ctcggtgaac gtcatcggtg cgctccgcgg caccgatcca gcgcttcgcg acgaatacgt
      361 gctttacagc tcgcatcagg acgccaacgg cgtgcggctg ctggcagccg gcgactcggt
      421 gctcgcgggc gccgacgaca acgcgtcggt cagtgtcgcc cagtttgcgg cagcccgcgc
      481 cttcgtcgcc cagcgcccca agcgctcgct cctcttcgtc caccacggcg cggaagagcg
      541 tggtctcctg ggctcgcggt accactcggc gcatccggtc gtgccgttgg ggcagatcgt
      601 gaccgtgctc aacggcgaca tgatcggccg caaccatccc gacacggcga cgctgctcgg
      661 cgcgcagccc ccgcatcgca actcgagcga gctcgtcgcg atggcgctcc aggccaacgc
      721 ggcgacagga cgattcgtgg tcgacaccct gtgggaccgt cccactcaca ccgagggatg
      781 gtacttccgc agtgaccacc tcccgtacgc acgactgggc attccggccc tgatgtactc
      841 gaccaatctg cacgacgact accataccgc cggggacctt cccgagcgga tcgactatgc
      901 caagcttacc cgcatggcgc gctggatgta cctcaccggg tggtacgtcg ccaacgcgcc
      961 caggcgaccg gcgttggatg cgggattcaa gctggagcgc gagtaatgcg aaacatttca
     1021 gacggtgtcg ttcttgagga tctcgtccgt cgcctgcgcc gcctggctcc caccctgccc
     1081 ggcctctggg gaacgatgaa agcgcagcaa atggcgctgc acgtgggaga cgcctgcgcc
     1141 gcggtactca agcagcgcga cttctccgcc aagccgcgca gcgggccggc cgggctcctc
     1201 cgtattatcg tgctgtacct cctgccacgc atgccacgcg gcgtcaagag cggtgccaat
     1261 cctgccgccg ccgttgtcga gccggaggcg tttgcgcggg acctcgagcg tgcagtcacc
     1321 ctgctgcagc agctcgccac cgaagcaccc gacgggctcg tcgatcgcca cccgatcttc
     1381 ggccccatga cccgtgccga ttggatgcgc tgggcgtttc tgcacaccga tcaccacctg
     1441 cgacagttcg gcctgtaagc cagccggtgc cgggaacgct cgaggtgtcc actgcccagg
     1501 agactgcccg atgctgaggc tggcgattcc cgtactgcac gtgtcgagtt cgatggcggc
     1561 ggaacggttc tactgcgttg gcctcgggtt cctgcagcga ttcgcctatc gcccggatga
     1621 cgggcatgcc gatccgtgct acatgggact gatccgggac ggcgtcctgc ttcatctgtc
     1681 ttcatttccg ggcgacggcg ttgccggggg cgtcgccaat ttcatcgtcg acgaccttga
     1741 cgcgttgcac gaagagtttc gccgcaaagg cctccggatc gagctcgagc cgacggacca
     1801 gagctgggga aatcgtgaga tgtacctgga ggatgccgat ggaaacagca tccgctttgt
     1861 cttgaacacc ggcgcgtaac ggacggacca acccgggccc cggcgcatgc cccgacgacg
     1921 atcccctcag aaccaggacg cgctgaatgt ccgcgaatac gacggttgtt ctcgctgcgc
     1981 tcgccgctgt gattctcatt gcagccacct gctacctcgc gtttcagctt cgcttcgagc
     2041 gttggaagag ggatcacacc aaagcggccc gacgtgacgc cgttcaacgc agccaggccg
     2101 tcaccctggg caagatctac gagcacctca tcccgtactt gccggatttt cgctggaatc
     2161 ccaaggacgc ccggttcatc gggacgccag tcgatttcct ggtcttcgac ggactgagcg
     2221 aaggtcaggt gcgcgccatc atattcgtcg aggtcaagac gggcacctct tccatgagtg
     2281 tgcgcgaacg ccaagtccgg gacgcggtgt tgggacagcg agtcgagtgg catcaactca
     2341 acctgcccga tgtccgaatg cggccggacc ccgcggcctg acgaatcgat gaagacgttt
     2401 cgggcacaga ccctggacca atggcgcgac tggctcgccg cgcaccatga ctcggagtcc
     2461 gaggtatggc tcatttttca taaagtgcac accggagtcc cttcgatcgc ctatcaggat
     2521 gcgctcgacg aggcgctgtg ctttggctgg gtggacagcc tggtcaagcg cctggatgag
     2581 cgccgctatg cccgcaagtt tacgccgcgc acagcgaaca gccggtggtc cgccataaac
     2641 cgaacgcgtt acgcggcact caaagccagc ggacgattga gggcggccgg actccgtcga
     2701 gccccgacag agcgcggcca tgatccgcgc ccatcccgat tcacgatgcc ctcgaagctg
     2761 ccgccctata tcgcggccgc gttgcggaag cggccggcag caatgcgcca cttcgaggcg
     2821 ctggcgcctt cgctccgccg gaattatgtc ggctggatcg agtccgccaa gcgggacgaa
     2881 acgaaagccc ggcgtctgaa agaggcgatt cgcctcctgg ccgccgggaa ggagctcgga
     2941 ctcaaatgag cctcccggct ggcctgcagg agcaattcgg cgagatcgac atctacctct
     3001 tcgaccagtt gttgcgaggc cgcattgtgc caggcatgcg gattctggat gccggctgcg
     3061 gctcgggtcg caacctcgtc tatctgctgc aagcggggta tgaagtcttc ggcgcagacg
     3121 aggatgccgc ctcgattgcc gaagtgcgtc gcctggccgc acgattcgct ccagcacttc
     3181 cggcagacaa cttccgggtc gagtcgttgg aggcgctgac cttcccgagc ctcttcgcgg
     3241 atacggtgat ctgcagcgcc gtcctgcact tcgctgcaga cgatgcgcgt ttcaacgcta
     3301 tgctgcaagg ctgttggcgg gtgctcaagc cgaacggtct gtttttctgc aggctggcat
     3361 cgtccatcgg cttggacggt cggggagaac ccctcggcgg acgccgttac cgattgccag
     3421 accgctcgga gcgatatctg gtggatgagc cgctgttgct ggccctgacc cgaggcctgg
     3481 gcggagagct ggtcgacccg ctcaagacca ccgtcgttca agaccagcga tgtatgacga
     3541 cctgggtggt acgcaaagcg ctctcggacg gagcgccatg agatgacact cgatattcat
     3601 gaccgtgttg ccgcgccggc tatcttccct gcaatgaccg acacctcaaa gcgctcccga
     3661 cgctggatca cctacgcggc ctgcggctgg gccgttctct ttgccgcacc acacacctgg
     3721 tgggccctcg gcatcccggc agggttcccc gggggagagg caaaccacca gctgatgatg
     3781 acgagtgcct ggcgatac
//
LOCUS       DASRJQ010000419        10317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2144818, whole genome shotgun sequence.
ACCESSION   DASRJQ010000419 DASRJQ010000000
VERSION     DASRJQ010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 10317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10317
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2144818"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1443)
                     /locus_tag="VFB89_09435"
     CDS             complement(<1..1443)
                     /locus_tag="VFB89_09435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HZM27568.1"
                     /translation="MSVPGSSSSRRSAQPPLSSYLISHPYAFSFGLLALGLGLRWLMT
                     PLVGIRLPYITLFPVVFAVAWYAGFRPTLLVTIVGALAVQYFLFPPYFTFRLDDSVGM
                     VGTGLFALIGVGAGLMGEARQKALIAANEAARRADEERERAEDEAAKAEEAAVEAEEA
                     AQEAAEALSARLAAEAETRRIESELSEFFENAVVGLHWEGPDGIISRANRAELEMLGY
                     AASEYVGRHIAEFHVDRAVIDDILRRLHADEKLRDYPSRMRCKDGTIKDVLIDSSVYW
                     EDDRFIHTRCFTRDVTVQRRTEEAMRALQRLESVGRLAGGVAHEVNNQMSVVLGAADF
                     VLRRDDVPPEVRADVQLMREAAQRSSGITSQLLAFGRRQLLKPVVLDLKDVIRNFEPV
                     LLRTAGSQCSVRVRLDDPGGNARVDRNQLEQVLLNLAINAVDAMPQGGALEVRLGPAE
                     LVADDPRLRYEPSVRPGQYAEIAVADTGTGM"
     gene            1641..6752
                     /locus_tag="VFB89_09440"
     CDS             1641..6752
                     /locus_tag="VFB89_09440"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HZM27569.1"
                     /translation="MARTAFRVLLGVLATGLLLVLVGSLVLYAGGATWLANHFLGVAV
                     AKLPGTTLRVRSVGGTLFTGLVVHDLVLDRGAAGAPLHVDEVRVRYHLPAVLQRDIAI
                     DEVRIIRPSVSLFQLRDSSWNFLPPVPKTGSPSTGPGPTIRIELVSVSRGQATVQFLG
                     GRAGPTLRVEDLELRARDVRVGSEVELRLDSLGLRALPPGPSPQWVSVTAAGAARGGL
                     VELARLDLRSPSSFVSLSGRATMPQLKWRQARDVDLTLSARPLSYRDLRALGSRFERD
                     EAVTVALRAQGSSSRATVNVEAISSNGGSLAMNGHITPPGVSPIDYDLHGRARNLDPG
                     WLSGSVTRTGSLNADFDVTLGGPSLDRLGGTARARATGSRYGSVASRTLGLDARFTEG
                     KATVESRGDAAKVGFSAAGWVRPFDSIPSYSMRGQLRAGAASEAAPLLERLTGPDGLR
                     IRFQADGRGLNPKQANATLVATSVPSEVRQALLDSARVKVSLRNGIATAEAAAGLTSG
                     LVRVRANVWLEPEIRYRIDRGTIEGVDLMAVLGDTMGSRINGSISLYGRGLEPSRANL
                     DGLVQVSRSSLGLHDLDEGRVDLKLRGGVVQLSGDVSLDGADLTLLADATPFSTSREV
                     RVREVRFRHIDLARTAPGLRLSSDLSGTATLSARGRGAKSLVLSTRINLEPSRLGAQS
                     LDRGTFEGTSDRGKVAFRSALEARAGYVKLAGRGRPFDSLPSFELTQGDFHDLDLGLV
                     LGRPELETRLTGRLDGRARGRKVESMEATALLQLDSSMVKDRTVRTGRVHATLDHGKV
                     QLVGRLLTDADSLDVNASGEPFLERPRFRLTTNLNVPAVAALLGTTSIDVGGTARLEA
                     EGELGPRDSMQLAGRLSLQGHYAGVKVDSADAALALASGTLHVDTLRVRSNVGEVMAQ
                     GPVVLFPRSATEASDLKIEARLADLAPLGPLLELDTIGVDSAGLSGSVKGPPGRRELA
                     LQATATGLAVSNSRIGSLNAAVTGTIEGDSSFGPGTGTLALAGFSRGGTLVRSIRLRG
                     DYDGSELALQTESIIDDRRSIRLAGRWNPRPDQQRVHLDSLVVQGRERAWALAHPAVI
                     TYGDRLSVGDLVVASGNRRIAIDGVIDRRGEQNFHARIDSLPLKGLTEFSSFSALDGE
                     VNGRFDLVGPAEAAHATGDVSIALKPRGKPVGRAAGRLDWDSAGLRLGLGLLPHKGDS
                     VSIRGDIPVALSLVRGDSTARLVRPIPNGPLSMDAESKAFPLATLEPLLDPSVARDFR
                     GTLSLDVKTRGTLEDPRLSGNIGLTDARVRLPQLGTTYDHGQATIVLDHREIRLSELR
                     LQAGSGRLTAKGTVQLKESSLATGPGATYDLDASLHEFPVIGSKDLRSKLTGDLHLGG
                     TSEGPVLAGAITAQNTDFYLTARSSEHAASDVTLTPADLRVVERRFGYAAARSEKRKG
                     QRFEEAALDLDVKLGSNIWVRRRSDPVTAVELSGEVKIKKERGQPVNAFGAIKPVPGR
                     SFVELLGRRFNLQGGAVDLNGPLETARLQLHTEYRPSSQSSTSASDVVITADVEADTG
                     KIAVVLGSTPLMEQRDIMSYVMTGGPATTNPTVTTGNQTSGVATGTQLAVNAALGTVA
                     GSAGQKLGLDVVQILQDLQGAQTLVAGKYVNPSLYLGVRQPLVQSTNSRDTSPETGTN
                     VMEFEVEYAAFQDVLLNLQGAGSEVRVFLRLRSGY"
     gene            6814..8931
                     /locus_tag="VFB89_09445"
     CDS             6814..8931
                     /locus_tag="VFB89_09445"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HZM27570.1"
                     /translation="MVAEPGSAQDSLIIVRPGAQVGSVDFKFTGKHSFLTSELNRQIE
                     LRGRGSFYGFRRLVGKLPFISEPASSSFDPIELQKDVVRLRRFYQQSGFVRPEVDYRV
                     RTRSDGSLVDVDFVINEGPSVVLRNVTTVGPTRGPGPNIPDSLAGEWRALLADIGLAR
                     EHRFGEAEGRSIEQQVVTWLRNRGYPDAAVRSSLAVDSVRDAADLTLQVDPGPRRRIG
                     VITVDGNTSVSDQVVLREFSPRSGDWFSVNDLAQGRSRIQQVALFRQVALDVPPEATT
                     DSTVSVRIRVTEAHPRLTLAEIGYVSDGAGLTGRVQWTHPNFTGGARSLTASVEAGTG
                     TGAIQSRSERFVRTSLTLTQPYVFTSGLLGTVGPFAEYRDDYRDQSVALGVSAALVRR
                     LGNLSSIALQYQFSGRHIFEYRGGEVSPGNISLLTLLSLQDPTLIDSLGTNEQLSTLG
                     IFGTLGKVDNVVDPHEGFLFRPSARVTVPAGLNTSQFFRLDLAATRFQPLSRNVVLVG
                     RASFGRLFPFGKSIPPPGGDPTFSFLHLRDQSMTAGGTDDVRGWGTGLLGPKVPNVQG
                     TVVGNDTVLTADRWVPVGALARVTGSVELRFPAPGLSPNWGTHLFIEGGRVWTPDERF
                     SVHPFLPDSTDFRFSAGAGLGYQTPVGAVRFSIGYKLNPSLADTRDAGEVLDSAKVGK
                     PVTSVPSHWIDRLQLHFSLGLAL"
     gene            8976..9986
                     /locus_tag="VFB89_09450"
     CDS             8976..9986
                     /locus_tag="VFB89_09450"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HZM27571.1"
                     /translation="MRITLASLVLLLAAEAAEAQKTDSVWIRNGDHITGEIKSLSRAL
                     LEYSTDDLGTIYIEWDKVERITSPATFEVQLISGKKFYGPLGLAPAGLVVVEADTLLL
                     REIVGIAPIKGSLLARLSGYFDLGFSYQKAHETVQLTTGTKVMYRGTLVETTFELTTF
                     IEDRDDAVETSRLSTGLTERFLFGERWSAGLGIGFDRNDELDLAGRGRIIGFGSRALA
                     QSNHIDFRASGGLMVTRERYFSSDTTSHGFEGVVTVEFNAFRYDRPKLSASVKSQAFP
                     SFTVKGRIRLQNDLRVSYELVKDFMLTATLFDAYDNKPQAVGAPKNDFGTTLAISWSF
                     GD"
     gene            complement(9992..>10317)
                     /locus_tag="VFB89_09455"
     CDS             complement(9992..>10317)
                     /locus_tag="VFB89_09455"
                     /inference="COORDINATES: protein motif:HMM:NF036996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-asparaginase"
                     /protein_id="HZM27572.1"
                     /translation="YENMDGGLIDAAVASGAKGLVIAGVGNGNMTEAAIKALAAQAKK
                     GIVCVRSTRVATGRVGRNVEVDDDKNGFVASLGLNPQKSRVLLRLALLRTKDVKTIQR
                     YFDEY"
BASE COUNT         1797 a         3279 c         3343 g         1898 t
ORIGIN      
        1 catgccggtc ccggtgtcgg ccacggcgat ctcggcatat tgaccaggtc gcacggaggg
       61 ctcgtagcgg agtcgcgggt cgtcggcgac gagctcggcg gggccgaggc ggacctcgag
      121 ggctccaccc tgaggcatcg catcgacggc attgatggcc agattgagca gcacctgctc
      181 cagctggttc cggtcaaccc tcgcattgcc cccgggatcg tccagccgca cacgaacgct
      241 gcattggctg cccgcggtcc ggagcagcac cggttcgaag tttcggatga cgtctttcag
      301 gtcgaggacc acgggcttga gcagctgccg ccggccgaaa gcgagcagct gactggtaat
      361 ccccgaggag cgctgggcgg cctcacgcat cagctgcacg tcggcgcgca cctccggagg
      421 gacgtcgtcg cggcggagca cgaagtccgc agcgccgagg accacgctca tctgattatt
      481 gacttcgtga gccacgccgc cggccagccg gcccaccgat tcgagacgct ggagtgcgcg
      541 catcgcttcc tcggtgcggc gctgcacggt tacgtcccga gtgaagcagc gggtatgaat
      601 gaatcgatcg tcttcccagt acacgctcga atcaatcagc acatccttga tagtgccgtc
      661 cttacaccgc atgcgcgatg ggtagtcacg gagcttctcg tctgcgtgga ggcgacggag
      721 tatgtcgtcg atcaccgcgc ggtcgacatg gaattcagca atgtgccggc ccacgtattc
      781 gctggccgcg tagccgagca tctccagctc ggcacggttg gcccgcgaga tgattccgtc
      841 gggcccctcc cagtgaagcc cgaccaccgc attctcgaag aattcgctca gctcgctttc
      901 gatccgccgg gtctcggctt ccgcggcgag ccgggcactc aatgcctcgg cggcctcctg
      961 agctgcttcc tccgcttcga cggcggcttc ttcggccttg gcggcttcat cttcggcccg
     1021 ctcgcgttcc tcgtccgctc gcctggcggc ttcgttggcg gcaatcagcg ccttttggcg
     1081 cgcctcaccc atcaggccgg cgccgacgcc gatcagcgcg aagaggccgg tgcctaccat
     1141 tccgacggaa tcgtcgagcc tgaaggtgaa gtacggcgga aacaggaagt actgcacagc
     1201 cagggcgccg acaatggtga ccaacaacgt cggccggaat ccggcgtacc aggcgacagc
     1261 aaagacgacg ggaaaaagcg tgatataggg aaggcgaata ccgaccagcg gcgtcatcag
     1321 ccaacgcaga ccgagaccca gcgccagcag cccgaatgaa aaggcgtacg gatggcttat
     1381 gagataactg gagagagggg gttgggcaga acgccgcgag gaggaggatc ctggaacgga
     1441 caaatggcct cacctgatag gaagtagtgc cctgccgggt gggaactccg gctgcgctgg
     1501 actggagaaa gatagctcgc ctgggttgac ccgcgaggcg ggtcgagagg tggcacgagg
     1561 ccccgttcgg agttcaattt agccgttgtc ccaacgcgac ttcccggcga agatcgagcg
     1621 cctctacacg gcacactttc gtggcccgca ccgcgttccg cgttctactt ggagtgctcg
     1681 cgaccgggct cctgctcgtc ctggtcggga gcctggtgct gtacgccgga ggggccacct
     1741 ggctcgcgaa ccacttcctt ggagtggcag tcgcaaagtt gcctggaacg acgctccggg
     1801 ttcgatccgt gggtggaacc ctgtttaccg ggctggtagt gcacgacctg gtactcgacc
     1861 gcggggcggc gggtgctccc ctccatgtgg acgaagtgcg tgttcggtac catctgccgg
     1921 cagtgttgca gcgggacatc gcgattgatg aagtccggat catccggcct tcggtgagcc
     1981 ttttccagct gcgcgactct tcctggaatt ttcttccacc agttcccaag actggatcgc
     2041 cgtccactgg gcccggcccc acgatacgga tcgagctggt ctcggtatcc cgcggccaag
     2101 cgaccgtgca attcctgggt ggccgggccg ggcccaccct gcgagtagaa gatctcgagc
     2161 tgcgcgcacg ggatgtccgg gtgggatcgg aggtcgagct tcggctcgac tcgctcggtc
     2221 tccgagccct cccaccggga ccgtcgccac agtgggtcag cgtcacggcg gcgggtgcag
     2281 ctcgaggcgg actcgtcgag cttgcgcggc tcgacttgcg ttcgccctcg agcttcgtct
     2341 ctctcagcgg ccgggccacc atgccccagc tcaagtggcg ccaggctcgc gacgtcgatc
     2401 tcactctttc tgcgcgacca ctctcttatc gcgacctgcg ggcactggga tcgcgcttcg
     2461 agcgggacga agcggtgacc gtcgccctgc gagcccaagg ctcaagcagc cgggcaaccg
     2521 tcaacgtcga ggcgatatcg tcgaacggcg gaagtctggc gatgaacggg cacatcaccc
     2581 cgccgggcgt gagtccgatc gactacgacc tccacggacg cgcgcgcaat ctggatcccg
     2641 gttggctcag cggcagcgtc acccgaaccg gctccctcaa tgccgacttc gacgtcacac
     2701 tcggcggacc cagcctggac cgcctcggcg gaacggcgcg ggcccgcgct accggctcgc
     2761 gctacggcag cgtggcctca cggaccttgg gcctcgatgc gcgtttcaca gaggggaagg
     2821 caacggttga gagccggggg gacgcggcca aagtgggctt ctcggcggct ggatgggtgc
     2881 gccctttcga ctctatcccc tcctatagta tgcggggcca actgcgcgcg ggcgccgcca
     2941 gcgaggctgc cccactgctc gagcgcctca ccggtccgga tggtcttcga atccgcttcc
     3001 aggccgacgg acgcggcctc aatccgaagc aggctaatgc cacgctcgtt gctaccagcg
     3061 tgccctccga agtcaggcaa gcgctgctcg actccgccag agtcaaggtg agccttcgga
     3121 acggtatcgc gactgccgag gccgcggccg gtctcaccag cggactcgtc cgcgtgcgcg
     3181 caaatgtctg gctcgagccc gagatcaggt atcggataga tcggggaacg atcgagggcg
     3241 tagacctcat ggcggttctc ggtgacacga tggggagccg catcaacggc agcatcagcc
     3301 tttacggccg tggcctcgag ccgagccgtg caaacctgga cggcttggtt caggtctcac
     3361 gctcgagctt gggtttgcac gacctggatg agggccgggt cgatctgaag ctccgcggtg
     3421 gagtggtgca actcagcggg gacgtgagcc tcgacggcgc cgacctcacg ctgctcgcgg
     3481 atgcgacacc gttctcgacc agtcgcgagg tacgagttcg ggaggtgcgc ttccgacata
     3541 tcgatctggc tcgaacggcc cccggtctcc gcctttcgag cgatctgtcg ggaactgcaa
     3601 ccctcagtgc gcgcggccgc ggtgcgaaga gtctggtgct gtcgaccagg atcaacctcg
     3661 agccctcccg gttgggtgcc cagtcgcttg accgtggcac gttcgagggc acctcagatc
     3721 gcggtaaggt agcgtttcgg agtgcgctcg aggcgcgggc cggttacgtc aagcttgcgg
     3781 ggagggggcg cccgttcgac tcactgccga gcttcgagct cacgcaaggt gacttccacg
     3841 acctggatct ggggcttgtg ctgggtcggc cggagttgga gaccaggctg accgggcggc
     3901 tcgacggacg ggcgcgtggc agaaaagtcg aaagcatgga ggcgaccgcc cttctgcagc
     3961 tggacagctc catggtgaag gaccgaaccg ttcgaaccgg ccgagtgcac gccacgctcg
     4021 atcatggcaa agtccagttg gtgggccggc tcctcacgga cgccgatagc ctggacgtga
     4081 atgcatccgg cgagccgttt ctcgagcggc ctcggttccg cctcactacc aacctgaacg
     4141 tccccgctgt cgctgccctc cttgggacaa cctcaattga tgttggcggg acggcgcggc
     4201 tcgaggcgga gggggagctc ggtccccgtg acagcatgca gctcgcgggc cggttgagtc
     4261 ttcagggtca ttatgcaggt gttaaggtgg attcagccga tgctgcgctc gcactcgcca
     4321 gcggcacact gcacgtggat acgctgagag tacgatcgaa cgtgggcgag gtgatggccc
     4381 aaggaccggt cgttctgttt ccccggagcg caacggaggc ttcggatctc aagattgaag
     4441 cacgactcgc agatctggct ccactcggac cattgctcga gctcgacacc atcggagtgg
     4501 attccgccgg cctctcaggt tccgtcaagg gcccaccggg gcgccgggag ctggcgcttc
     4561 aggcaacggc caccggtctt gctgtttcca actcccggat aggatcactc aatgcggcgg
     4621 tgaccggcac aatcgagggc gatagctcgt tcggtcccgg tacggggacc ttagcgcttg
     4681 ccgggttctc gcgcggcggc acgttggttc gatcgatccg gttgcgaggc gactatgacg
     4741 gctcggagct cgctctccag acggagtcga tcatcgatga caggcgctcg atcaggctcg
     4801 ccggccgatg gaatcccagg ccggatcaac agcgggtgca tctcgacagc ctagtggtgc
     4861 aggggcgcga gcgggcatgg gcgctggctc atccggcggt gatcacctac ggcgatcgcc
     4921 tgagcgtggg agatctcgtg gtggcctcgg gtaatcggcg gattgccatc gacggtgtga
     4981 tcgaccgccg gggcgaacag aattttcatg cacggataga ttcgctgccg ctcaaggggc
     5041 tcaccgagtt ttccagcttc tcagcgctgg atggtgaggt caacggtcgt ttcgacctcg
     5101 tagggccggc agaggcggca cacgccaccg gtgacgtttc catcgccctc aagccgaggg
     5161 gcaagccagt cggccgagcc gcggggagac tggattggga ttcggccggc cttcgcctgg
     5221 ggttgggact tctcccccac aagggtgata gcgtaagcat acgcggcgac attccggtcg
     5281 cgctttcgct cgtccgcgga gactccactg cccggttggt acggcccatc ccgaacgggc
     5341 cattgagcat ggacgcggaa tcgaaggcct ttccgctggc caccctcgag ccactgctcg
     5401 acccgagcgt cgcgcgcgac tttcgcggta cgctgtcgct cgacgtcaag actcgcggca
     5461 ccctggagga tccgcggctg tccggcaaca tcgggctgac ggatgcgcgg gtccgactgc
     5521 cgcagctcgg cacgacatac gaccacggac aggcaaccat agtgctggac catcgggaga
     5581 ttcggctttc cgagctgcgc ctacaggccg gcagtggaag gcttacggca aaaggcaccg
     5641 tccagctcaa ggagtcatcc ctcgcgactg gaccaggcgc cacgtacgac ctcgacgcat
     5701 cgctgcatga atttccggtc attgggtcca aggacctgcg atccaagctc acgggcgatc
     5761 tgcatctcgg tggcacctcg gaagggcccg tcctcgccgg tgccatcacc gcgcagaata
     5821 ccgacttcta tctcacggcc cgaagctcgg agcacgcggc cagcgacgtc acattgacac
     5881 cggcggacct gcgcgtggtc gagcggcgct tcggctacgc ggctgcccgt tctgagaagc
     5941 ggaagggcca gcgtttcgag gaagccgctc tcgatctcga cgtgaagttg gggtcgaaca
     6001 tttgggttcg ccggcgcagc gatccggtga ccgccgtgga actgagcggc gaggtcaaga
     6061 tcaagaaaga gcgcggccag ccggtcaatg cctttggtgc gatcaaaccg gtacccggcc
     6121 ggagctttgt cgaactgctg ggaagacgct tcaaccttca gggcggggcc gtggacctga
     6181 acgggccact ggaaacggca aggctccagc tgcataccga ataccgccct agctcgcaat
     6241 cgagcacgag cgcctccgac gtcgtgatca ctgcggatgt agaggcggac accggaaaga
     6301 tcgctgtggt cctggggtcc acgccgttga tggagcagcg cgacatcatg tcgtatgtaa
     6361 tgaccggcgg gccggccact accaacccga cggtcacaac cggcaaccaa accagtggcg
     6421 tcgcgaccgg tactcagctg gccgtcaacg ctgcgctagg gacggtggca ggaagcgccg
     6481 gccaaaagct gggcctcgac gtggtgcaga ttcttcagga cttgcaaggc gcccaaacgc
     6541 ttgtggccgg caaatacgtg aatcccagcc tctacctcgg tgtgcgccag ccgctggttc
     6601 agtcaacgaa ctctcgggac accagcccgg agaccggaac caacgtcatg gagttcgagg
     6661 tagagtacgc cgcgttccag gacgtgctgc tcaaccttca aggagccggc tccgaggtcc
     6721 gggtgttcct ccggctccgg agcggctatt gatctgccgc ctgccgcccg cccgctccac
     6781 ctgcaactgc tcatcctgat cgtcggcgga ctggtggtgg ccgagccggg gagcgctcag
     6841 gattcgttga tcatcgtgcg tcccggtgcg caagtcggct ccgtcgactt caagttcacc
     6901 ggcaagcaca gttttctcac ctctgagctc aatcggcaga tcgagcttcg gggtcgaggt
     6961 tcgttttacg gcttccgcag gctggtcggc aagctgccgt tcatctcgga gcctgccagc
     7021 tcttcgttcg atcccatcga gctccagaaa gatgtcgttc gactgcggcg attctatcag
     7081 cagtccggct tcgtgcgccc cgaggtcgac tatcgagtgc ggaccaggag cgacggctct
     7141 ctggtggacg tcgacttcgt catcaacgag gggccaagcg tagtactgcg caatgtcacg
     7201 acggtcggcc cgacgcgagg tcccggtccg aatattcccg attctttggc aggggagtgg
     7261 cgggccctcc tggcggatat cggtttggcg cgggagcacc ggttcggcga agcagagggc
     7321 cggagcatcg aacagcaagt cgtcacctgg ctccgaaacc gtggctaccc cgacgcggca
     7381 gtccgctcga gtctggcggt cgactccgtc cgggacgccg ccgacctcac ccttcaggtg
     7441 gatccaggtc caaggcgacg gatcggcgta atcacagtgg acggaaacac atccgtcagc
     7501 gaccaggtcg tgctgcgcga gttctcgccg cgctccggcg actggttctc cgtgaatgat
     7561 ctcgcgcagg gccgctcccg gattcagcag gtcgctctct tccggcaggt agcgctcgat
     7621 gtgccgcccg aagccaccac cgattccacc gtgtcggtgc ggatccgggt gacggaagcg
     7681 catccgcggc tgacactcgc cgaaatcggc tacgtctccg acggagccgg gctcaccgga
     7741 cgggtgcagt ggacccaccc gaactttacc ggtggtgcgc gaagcctcac tgcatccgtc
     7801 gaggcaggga ccgggacagg ggccatccag agccggagtg agcgtttcgt tcgcacgtcg
     7861 ctcacgctca cgcagccgta cgtattcaca tccggactgc taggcacggt cggcccgttc
     7921 gccgaatacc gtgacgacta ccgggatcaa tccgttgctc tcggcgtttc ggcggcgctg
     7981 gttcgtcgcc tggggaacct gtcgtcgata gcgctgcagt accagttcag cgggcgacac
     8041 attttcgagt atcgcggtgg ggaggtctcg cctggaaaca tcagcctgct gacgctgctc
     8101 agcctgcaag acccgacgtt gatcgattcg ctcgggacga acgaacagct aagcacactc
     8161 ggcatcttcg gcacgctcgg taaggtggac aatgtggtgg accctcacga agggtttctc
     8221 ttccgaccca gcgccagggt cactgtccct gccggcctga atacatctca attttttcgg
     8281 ctcgaccttg cagctacccg gtttcagccg ctcagccgaa acgtcgtgct ggtagggcga
     8341 gcctcattcg gtcggctctt tccattcggc aagagtatcc cgccaccagg cggggatcca
     8401 accttctcct ttctacatct gcgcgatcaa tctatgacgg ccgggggcac cgacgacgtg
     8461 cgcggatggg gcacggggct gctgggtccc aaggttccca atgtgcaggg gacggtcgtg
     8521 ggaaacgata ccgtcctcac ggcggatcgt tgggttccag tcggcgcatt ggcccgggtg
     8581 accgggtccg tcgagctgcg cttcccggcg cccgggctct cgcccaactg gggcacgcat
     8641 ctctttatcg agggcggacg ggtttggact cccgatgaac gcttctcggt gcacccgttt
     8701 ctccccgact cgaccgattt tcgcttctct gccggcgcgg gcctcggcta tcagactcct
     8761 gtgggggcgg tgcggttcag tatcggctac aaactcaatc cctcactggc cgacacgcgt
     8821 gatgcggggg aagtgctcga ctcggcgaag gtggggaaac cggtaaccag tgtgccgagt
     8881 cactggatcg atcgtctgca gctccatttc tcgctcggtc tggcgctgtg agttcgcccg
     8941 acgcaaacag accccgctcc agatcaccgg gtttcatgcg catcactctg gcaagcctcg
     9001 tcctgttgct cgccgccgag gcggcggagg cgcagaagac ggattcggtc tggatccgga
     9061 atggcgacca catcaccggc gagatcaaat ctctctctcg cgcactgctc gagtattcca
     9121 ccgacgacct gggaactatc tatatcgagt gggacaaggt cgagcggatc acgagcccgg
     9181 ccactttcga ggtgcagctg atctcgggca agaaattcta tggaccgctt ggcctggccc
     9241 cggccgggct agtcgtcgtg gaggccgaca cgctgttgct gcgggagatc gtggggatag
     9301 ctccgatcaa gggaagtttg cttgcccgcc tgagtgggta ctttgatctg ggtttctcct
     9361 accagaaggc gcatgagacg gttcagctca cgaccggcac caaagtgatg taccgtggca
     9421 ctctggtaga gaccacattc gagcttacca cgttcatcga ggatcgggat gatgccgtgg
     9481 aaacgtcgcg cctctcgacc ggactcacgg agcgctttct gtttggggag cgttggagtg
     9541 ccggattagg cattgggttc gaccggaacg acgagctcga cctggcaggg cgtggacgga
     9601 tcatcgggtt cgggtcccgc gcgctagccc aatccaacca catcgacttt cgagccagcg
     9661 ggggactcat ggtcacgcga gagcgatact tttccagcga tacgacgtct cacggttttg
     9721 aaggggtcgt gaccgtcgag ttcaacgcat ttcgctacga ccgccccaaa ctgagtgcct
     9781 cggtcaagag tcaggccttc cccagcttta ctgtcaaggg ccgaatcagg ttgcagaatg
     9841 atcttcgagt ctcgtacgag ctggtcaagg acttcatgct gaccgccacg ctgtttgatg
     9901 catacgacaa caagccgcag gcggtgggag ccccgaagaa tgacttcggg accacgctcg
     9961 cgatcagctg gagctttgga gactagtccg ctcagtactc gtcgaagtag cgctggatcg
    10021 tcttgacgtc tttggttcgc agcagcgcca ggcgaagcag cacccgtgac ttctgggggt
    10081 tgagccccag tgaggcgaca aagccgttct tgtcgtcgtc cacttcgacg tttcggccca
    10141 cccgtcccgt agccacgcgg gtcgagcgga cgcagacgat ccccttcttt gcctgcgcgg
    10201 ccaacgcctt gatcgcggcc tcggtcatgt tgccgttgcc gacgcccgca atcaccagcc
    10261 ccttggcccc gctcgccact gccgcgtcga taaggccgcc atccatgttc tcgtagg
//
LOCUS       DASRJQ010000420         7187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3688983, whole genome shotgun sequence.
ACCESSION   DASRJQ010000420 DASRJQ010000000
VERSION     DASRJQ010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3688983"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="VFB89_09460"
     CDS             complement(<1..865)
                     /locus_tag="VFB89_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZM27573.1"
                     /translation="MSDEPAKASDQPEVRYFFIRRPVFSAVVSIVVVLLGLFSLATLP
                     VNRYPPLTPPVVQVTAVYPGATAEDVAQAVAAPIEQQLSGLDGLLYFKSSSSSDGTLS
                     LSVYFDISRNVDLAAVDVQNQVALAEPQLPQEVVRQGIVVKKAQTDILLVTALQSDDP
                     RYNAEYLSNYSKIYVEDEIKRVPGVGDATTFGQLEFSMLLSLDPDRMTQLGLTVTDVA
                     NAVREQNTTNPAGRIGREPAPAGTQLTIPVTTLGRLKEARQFADIIVRARPDGSLVRI
                     GDIGKVTLGARN"
     gene            complement(862..2049)
                     /locus_tag="VFB89_09465"
     CDS             complement(862..2049)
                     /locus_tag="VFB89_09465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZM27574.1"
                     /translation="MRSLRYACLLIAVAACRKPAAAGPPPPEVTVLAVKPQTVPAKFE
                     WVAQAAASKSVEVRAQVAGVIVARPYVEGTDVKKGTVLFRIDPRTYEANYQSAQARVT
                     QSRATLANAQRNLARLEPLLAIRAVAKKDVDDAQTAVEEGEAALQDAEAAVKAAKKNY
                     DDTFVRAEIDGRAGRALMVLGALVSGPGDLLTTVEQVDPIYVLFSPSDQEVLTWRRDI
                     AAKRLIIPPGVLDVQATFSDGSVFGYKGKLNFIDIALRPSTGALQLRAEFPNPQHSLL
                     PGQFVRAHVLGARRTGAILVPQRAVQQGLNGPFVYLLGQAGKVSAQDVAASDWSGAEW
                     LIESGLKAGDQVIVDGTQKVAAGQPVRASAYRPETDTTLRARGDTAVVAPPAAPPPIR
                     ESK"
     gene            complement(2175..3095)
                     /locus_tag="VFB89_09470"
     CDS             complement(2175..3095)
                     /locus_tag="VFB89_09470"
                     /inference="COORDINATES: protein motif:HMM:NF027640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HZM27575.1"
                     /translation="MPQAVSAVAAAEPRRIGRTTLVRLAVYYVIAGAALLGLNRLVAE
                     LPTAVSEGKGPFPGWADAALQMISALVLVLPMALVYVRTRTRHKYDASLVQTVIVLPI
                     TVAAVLIVVQNSLALAFSLAGIVAAVRFRNTLKESRDAVYIFGSLGIGFSTGVHQLEI
                     ALVLSLLFSAVELVLWKYDIGVADHTETLCKFCGNEAGTQAEVVSTATPEQMNGAPGP
                     RDLKLRVFVADLGPARRALEPALTDLAKRWEFVGASEGEEDRYLDYTVHLKKRGSPDQ
                     LSGELLGRCGAQVVKLAIEPVSAEPVTPGH"
     gene            complement(3142..3555)
                     /locus_tag="VFB89_09475"
     CDS             complement(3142..3555)
                     /locus_tag="VFB89_09475"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZM27576.1"
                     /translation="MILSSAPRRMRDGVPEHPAVARVVAYLESSAGPVSLDSLADLVH
                     LSKSHLVRLFRSQMGLPPHAYLLRVRISRACRFLAAGMPIAEAAHAAGFADQSHLTRH
                     FKYYLGLTPGAYVRSLGATAPLGRPSTRTVDSAHG"
     gene            3920..5020
                     /locus_tag="VFB89_09480"
     CDS             3920..5020
                     /locus_tag="VFB89_09480"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27577.1"
                     /translation="MSMHPLVGHWDARRRLSRAFRADRLPQVLVLTGPAGVGKQRLGL
                     WLAALTLCAERGEEPCGKCASCRAVARLAHPDLHWFVPVPRPKATDREKQVEEATEAL
                     GQLLEERRAGSLYPPPDPMSSHGLASVRLLQRRASLTAVQGGKKVFILGDADRLVVQE
                     ASQEAANALLKLLEEPPTDSLFVLTTADPYRLLPTIRSRAVLLRVGRLSDNEVAEVFR
                     LAGNSETEVEAMHERIAVAEGSVGAALALDDVAGDAYQAAFEMLAAALAGGTAVPARM
                     ERALRQTPWAARGEFTAMLDALAETLSEAARAAVGETPRRPLPEALRNQRDAAALVRA
                     MERVAGAREAAAGNVNPQILLATLGEELAEVL"
     gene            5017..5499
                     /gene="moaC"
                     /locus_tag="VFB89_09485"
     CDS             5017..5499
                     /gene="moaC"
                     /locus_tag="VFB89_09485"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HZM27578.1"
                     /translation="MKLSHLDERGTARMVDVGGKPVTERTAQAAGAVRMSQEAFELVA
                     GNAIEKGDVLSVAKIAGTMAAKRTGELIPLCHPVGLDQVVVDVVLDPSLPGVRVQAAA
                     KVSGRTGVEMEALTAVAVACLTVYDMAKAADRGMVIEDVQLMSKTGGTRGDWRRPEEK
                     "
     gene            5532..6248
                     /locus_tag="VFB89_09490"
     CDS             5532..6248
                     /locus_tag="VFB89_09490"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HZM27579.1"
                     /translation="MRPPTRRKTMKQERTERMFWRGALVVAGALAIGALGGWTKRVRA
                     ADHKSAMDPSRLARQFGSLNDEVAAARGETNLLVVQLDRANKVMDYSTKYQIPADLAA
                     AIYDVALSEGIDPRLGFRLVKVESEFKRDARSPTGALGYTQLQPATARFYEAGITESG
                     LLERDVNLRIGFRFLKELLGQFHNDMHLALLAYNRGPSKVTEILSQGGDPKNGYSDAV
                     LRGYRPPHTETGDGGMTTWR"
     gene            complement(6245..>7187)
                     /locus_tag="VFB89_09495"
     CDS             complement(6245..>7187)
                     /locus_tag="VFB89_09495"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZM27580.1"
                     /translation="DTIPSDATFFRLYDTKLLRRETKDYVPKLIAAAMIAKEPTKYGF
                     PALSPEALTPYDSILVPDQTGLDVIARLADTTIAALRELNPQYLRLATPPGRESVVRL
                     PPGAGERTTLAYADLPTSKRVSYREHVVRNGETLGTLAKRYGVGKADIIAANPRIRGN
                     TIHAGQNLVIPTLGPLPAEVTRQLATETTDADFHRVRRGENLGSIAARYSVSKRQLQA
                     WNHMGKSTRIRAGQRLRVSAPEVQFRVATRSTAKHSGNPAASNGTTGGRSHVVRQGDT
                     LSGLARRYGVSVQALRAANGLSDDATLRRGVRLKIPA"
BASE COUNT         1301 a         2221 c         2394 g         1271 t
ORIGIN      
        1 agttgcgtgc gccgagggtc accttgccga tgtcaccaat acgcacgagc gatccgtccg
       61 gccgtgcccg aacgatgatg tccgcgaact gcctggcctc cttgagtcgg ccgagcgtgg
      121 tcacggggat ggtgagctgc gtgcccgccg gtgccggctc gcgtccgatc cgccccgcgg
      181 gattggtcgt gttctgttcc cgcaccgcat tcgcaacgtc cgtgacggtg aggccgagct
      241 gcgtcattcg gtccgggtcc aggctcaaca acatcgagaa ttcgagctgc ccgaaggtcg
      301 tggcatcgcc cacgccaggc acccgcttga tctcatcctc gacatagatc ttcgagtagt
      361 tgctgagata ttccgcgttg tagcgcggat cgtccgattg gagcgcggtg accaggagaa
      421 tgtcggtctg agccttcttt accacgattc cctggcggac cacttcctgc ggcagctggg
      481 gctcggccag cgcgacctga ttctgcacgt cgacggccgc taggtcgacg ttgcgcgaga
      541 tgtcgaaata caccgacaag ctcagtgtcc catcactcga gctcgacgac ttgaagtaga
      601 ggaggccgtc gagcccggag agctgctgct cgatgggcgc ggcgactgcc tgcgccacat
      661 cctctgccgt ggcgcccggg tacaccgcgg tcacctggac caccggcggg gtaagcggtg
      721 gatatcgatt cactggcaga gtcgcgagcg aaaagagccc gagcagaacg accacgatgg
      781 agacgacggc cgagaatacc ggacgccgga taaagaagta gcgaacttca ggctggtcgc
      841 ttgccttggc gggttcgtcg ctcacttcga ctcccgaatc ggcggcggcg ccgccggcgg
      901 tgccaccacg gcggtatcgc ctcgtgcccg aagggtcgtg tcagtctcgg gcctgtacgc
      961 ggaagctcgg accggctggc ccgcggccac cttttgagtg ccgtccacga tgacttggtc
     1021 gcccgccttg agaccgctct cgatgagcca ctcggccccg gaccagtcgc tggcggcgac
     1081 atcttgtgct gagactttgc ccgcttgccc cagcaggtag acgaacggtc cgttgaggcc
     1141 ttgctgcacg gccctctgag gcacgagaat cgcccccgtg cgcctggcgc cgagtacgtg
     1201 cgcccgaaca aactggcccg gcaacaggct atgctgcgga ttggggaact cggcccgaag
     1261 ctggagcgcg ccggtggagg gccgaagcgc gatgtcgatg aagttgagct tccccttgta
     1321 gccgaagacg cttccgtcgg agaaggtagc ctggacatcg aggacgcctg gcggaatgat
     1381 cagccgcttg gcggcgatgt cgcgtcgcca ggtgagcacc tcctggtccg agggactgaa
     1441 caggacgtag atcggatcga cctgctcgac cgtggtgaga aggtcgccgg gcccggatac
     1501 caacgcaccc aataccatta gagctcggcc cgcccgtcca tcgatctcgg cccggacgaa
     1561 cgtgtcgtcg tagtttttct tggcggcctt cactgccgcc tcggcgtcct gtaaggcagc
     1621 ctctccctcc tccacagccg tctgggcgtc gtcaacatcc ttcttcgcca cggcgcggat
     1681 tgcgagcaac ggctcgaggc gggcaaggtt ccgctgagca tttgcgagcg ttgcccggct
     1741 ttgggttact cgggcctgtg cgctctgata gttcgcctca taggtgcgcg ggtcgattcg
     1801 gaagagaact gtgcccttct tcacgtcggt gccttcaacg tagggccggg cgacgatcac
     1861 tccggccacc tgcgccctca cctcgacgct cttcgaagcg gcggcctgcg ccacccactc
     1921 gaacttcgcc gggacagtct gaggcttgac ggccagcacg gtgacttcgg ggggaggcgg
     1981 gcccgccgct gcgggtttac ggcaggcggc aaccgcgatc aacaggcagg cgtatcggag
     2041 cgacctcacg aaactgcttc tccccaacgg gcggaggtcg agtggatgtg gcaccgcgtc
     2101 gcaacctttc tctagcgggc gcatgaacgg agggcaggtg tcgatgtgaa ggagtcgtgg
     2161 accgccaacg gcgattaatg cccaggggtt accggctcgg cggagaccgg ttcgatagca
     2221 agtttgacca cctgggcgcc acagcgcccg agcaactccc cgctcaactg gtccggcgat
     2281 ccgcgcttct tcagatgcac ggtgtaatcg agataccggt cctcctcgcc ctctgaggcg
     2341 ccgacgaact cccaacgctt ggcgaggtca gtcaatgcag gctcgagggc ccgacgggct
     2401 gggccgaggt cggcgacgaa aacacgcagc ttgaggtcgc ggggtcccgg cgctccgttc
     2461 atctgctccg gtgtagcagt gctaaccacc tcggcctggg tgcctgcctc attaccgcag
     2521 aacttgcaga gcgtttccgt gtgatcggcg acgccgatgt cgtacttcca caagaccagc
     2581 tcgaccgccg agaacagcag cgacaagacc agcgcaattt cgagttgatg cactccagtg
     2641 ctgaacccga ttccgagcga gccgaatata tagaccgcgt cccgcgactc tttgagggta
     2701 ttccggaagc gaaccgcggc tacgattccc gccaggctga aggccagtgc caggctgttc
     2761 tgcaccacaa tcagcacggc tgccaccgtg atcggcaaaa cgatgacggt ctggaccaga
     2821 gaggcatcgt acttgtggcg ggttcgagtg cgcacgtaca ccagggccat aggaaggacc
     2881 agcaccagag cgctgatcat ctggagggcc gcatccgccc atcccgggaa tggacccttc
     2941 ccttcgctca ccgcagtcgg cagctccgct accagtcggt tcagaccgag cagcgccgcc
     3001 ccggcgatca catagtagac cgccagccgc accaaagtcg tccgaccgat tcggcgaggt
     3061 tcagctgccg caaccgcgct gaccgcctga ggcaccgagt ctcccttttt gatcggcttc
     3121 gggtccaaag ctaatcgtgg gctagccatg ggccgagtca accgttcgag tggacggacg
     3181 ccctaaagga gcggtcgccc ctagcgagcg aacgtacgct cccggcgtga gccccaggta
     3241 gtacttgaag tggcgggtca gatggctttg gtcggcgaaa cctgcagcat gggcggcttc
     3301 ggcgatcggc attcccgcgg ctagaaagcg gcaagcgcgg gagattcgaa cccggagcag
     3361 ataagcatga ggaggaagtc ccatctgact tcggaacaac cggacgagat gagacttgct
     3421 gagatgaacc agatcagcca acgagtcgag cgaaaccgga ccggcagagg attccagata
     3481 cgccacgaca cgggcgacag ccggatgctc cggcactccg tcgcgcattc gacgtggagc
     3541 tgaactcaga atcattggag cgcttgacgt catctgtggc gaactggtaa ggcctgggct
     3601 cgtgaccctg accgtcaacc gttttggcgg ccggaaggtt cactggcgcg ggcctggagc
     3661 gtagaccctt gcttcaccgc ctcccgctgt ccgcaagatt aaacccgact caggcttgcg
     3721 gaagagtgcc cctaggctgc tggagagcaa acgcccgcac tgctgcaaac gtgagcgagt
     3781 gctcgctgac aatttgacgg ctagaaacgg cgcaattgac cctattccat gcagtcatta
     3841 gcacgttaag tcattgttga aaaagatgtt acacagtttg accctaccga aatccagagc
     3901 cccacggtag gttctgccaa tgtcgatgca ccccctcgtc ggccactggg atgctcgccg
     3961 ccgactctcg cgagcgttcc gggctgaccg cttacctcag gttctggtcc tcacggggcc
     4021 ggcgggggtg ggtaaacagc gcctaggtct ttggctcgcg gcgctgacgc tttgtgccga
     4081 acggggcgag gaaccctgtg gaaagtgcgc gagttgtcgg gcagtagcca ggctcgctca
     4141 tccggacctg cattggttcg tgccggtccc ccggcccaag gctaccgacc gagagaagca
     4201 ggtcgaagaa gctaccgaag cgttaggtca gctcctggag gaacggcgtg ccgggagttt
     4261 gtacccgcct ccggacccga tgtcgagcca cggtctcgcc agtgtccggt tactgcagcg
     4321 acgtgcgtct ctgaccgcgg tgcagggcgg aaagaaggta ttcatcctgg gcgacgccga
     4381 tcgactggtc gttcaggagg cgagccagga agcagccaac gcgctgctca agctgctcga
     4441 ggagccgccg acggattctc tgttcgttct tacgacggcg gatccctatc ggttgctgcc
     4501 gaccatccgg tcccgcgccg tgctcttgcg ggtaggtcgg ctgagcgaca acgaggtagc
     4561 agaggtgttc cgcttagcgg ggaattctga gacggaagtc gaagcgatgc acgaacgcat
     4621 tgcggtcgcc gaaggatcgg ttggagcggc gctcgctttg gatgatgtcg ccggggatgc
     4681 gtatcaggcc gcgttcgaga tgctcgccgc ggcgctggcc ggaggtacag ccgtgcccgc
     4741 ccgcatggag cgggcgctgc ggcagacgcc ctgggcggcg cgtggagagt ttaccgcgat
     4801 gctcgatgcg ctggccgaga ccttgagcga agccgcacgc gcggcggtgg gagagacgcc
     4861 gcggcgtccg ctgccggagg cgctccgaaa tcagcgcgac gcggctgcgc tggttcgagc
     4921 catggagcgc gtggctggcg cacgtgaggc cgcagccggc aatgtgaacc cgcagatcct
     4981 gctcgcaacg ctgggcgagg agctggcgga ggtgctgtga agctctcgca cctggatgag
     5041 cgaggcacgg cccggatggt ggacgtcgga ggcaagccgg tgacggagcg caccgcgcaa
     5101 gcggccggag cggtcagaat gtcacaggag gcgttcgagc tggtcgccgg caacgcgatc
     5161 gagaaaggcg acgtactgag cgtcgcaaag atcgcgggga ccatggcagc aaagcgaacc
     5221 ggcgagctga ttccgctttg ccatccggtc gggctcgatc aggtcgtcgt tgacgtggtg
     5281 ctggatccgt cgctccccgg tgtacgggtg caggcagcag ccaaggtgag tggtcgtacc
     5341 ggggtggaaa tggaagcact cacggcagtg gcagtcgcgt gtctcacggt gtacgacatg
     5401 gcgaaagcag cagatcgggg aatggttatc gaagacgttc agctgatgag caagaccggg
     5461 ggaacccggg gcgattggcg ccggcccgag gagaagtgag cccggccggt tacccataca
     5521 ggaggacagc gatgaggcca ccaacccgta gaaagaccat gaagcaggag cgcaccgagc
     5581 gcatgttctg gcgcggcgcc ctggtcgtgg ccggagcgct cgcgataggc gctctgggcg
     5641 ggtggaccaa acgggttcgc gcggcggatc acaagtccgc gatggatccg agtcggctgg
     5701 cgcggcagtt tggctcgctc aacgacgagg tagccgccgc ccgcggcgag actaacctcc
     5761 tggtggttca gttggatcgg gccaacaagg tgatggacta ctcgacgaag tatcagatcc
     5821 cggcggacct cgcagccgcc atttacgatg tcgccctgag tgaggggatc gatcccaggc
     5881 tggggtttcg cttggtgaag gtcgaaagcg agttcaagcg tgatgcccgg agcccgaccg
     5941 gggcattggg ttacacccag ctccagccgg ccacggcgcg attttacgag gccggcatta
     6001 ccgaatcagg gctgctcgag cgggacgtga acctgcggat tgggttccgg ttcctgaagg
     6061 aactgctggg gcagtttcac aacgatatgc acctcgcgtt gctcgcatac aatcgcgggc
     6121 cgagtaaggt gaccgagatt ctctcgcaag gcggcgaccc gaagaacggg tactcagatg
     6181 cggtgctgcg ggggtatcga ccgccgcaca cagagacggg cgacggcggt atgacgacct
     6241 ggcgttaggc tgggatcttg agtcgcacgc cgcgccgcag cgtggcgtcg tcagacaatc
     6301 cgtttgctgc tctcagggcc tggaccgaga cgccataacg tcttgccagg cccgaaagcg
     6361 tatccccctg gcgcacgacg tggctccggc cgccagtcgt cccgtttgag gcggccgggt
     6421 tgccactgtg cttggccgtc gacctcgtgg caacccggaa ctggacctcc ggcgccgaga
     6481 cgcgcagccg ctgtccagcc cggattcggg tgctctttcc catgtggttc caggcctgca
     6541 gctgacgctt gctgacgctg tatcgcgcgg cgatcgaccc caggttctct cctcgacgta
     6601 ctcgatggaa atccgcgtcc gtcgtctcgg ttgcgagctg ccgcgtgact tcggccggga
     6661 gcgggccgag cgtcgggatg accagattct ggcccgcgtg gatcgtgttg ccgcggattc
     6721 tggggttggc agcgatgata tcggcttttc cgacaccata gcgcttggcc agcgttccca
     6781 gcgtctcgcc gttccgcacc acatgctcgc ggtaactgac ccgcttgctg gtcggcaaat
     6841 ccgcatacgc gagcgtggtc ctctcgccgg ctccgggggg caaacgcacg acactctccc
     6901 tgcccggcgg cgtcgccagc cgcaaatact gcgggttcaa ctcccggagc gcggcgattg
     6961 tcgtgtccgc caaacgggcg atcacatcca gccccgtttg atccggcacc aggatcgagt
     7021 cgtacggcgt cagtgcctcg ggtgagagcg ccggaaatcc gtacttcgtg ggttccttcg
     7081 cgatcatcgc tgcggcgatc agcttgggaa cgtaatcctt ggtctcgcgg cggagcagct
     7141 tggtgtcgta aaggcggaaa aacgtggcgt ctgacggaat cgtatca
//
LOCUS       DASRJQ010000421         7061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2031780, whole genome shotgun sequence.
ACCESSION   DASRJQ010000421 DASRJQ010000000
VERSION     DASRJQ010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7061
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2031780"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..859)
                     /locus_tag="VFB89_09500"
     CDS             complement(2..859)
                     /locus_tag="VFB89_09500"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HZM27581.1"
                     /translation="MIPASFEYTRATSLRDALTALAQGGGAKVLAGGHSLIPMMRFRL
                     AQPSRLIDIGRLSELKGVTEHRRGARIGALTSYREVLESDLIRERFPLIAEATEGIGD
                     LQVRNRGTIGGGLAHADPASDMPAIMVALDATFNLRSKRGKRSVPAREFFQGPFSTAL
                     AEDELFVEVLLPPLPRGAGTAYVSFNQAASGYSLVAAAAVIARSRLTVKHAVVALTGL
                     ADVARRVSAVDQLVGTKGEPDVISRVAAAAPEGLEIQGDVHAPAAYRRHLVTVATRDA
                     LTHALERAK"
     gene            complement(856..3213)
                     /locus_tag="VFB89_09505"
     CDS             complement(856..3213)
                     /locus_tag="VFB89_09505"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZM27582.1"
                     /translation="MATTLFGSGIKRREDPRLITGAATYTDDVKLPGLTFAAILRSPY
                     AHAKLARIDVSAARKAPGVLAVYTGADIKDRVVPVPCAWNPPNCDLKTPPHPLLAYDT
                     VRYVGDGVALVVAGTRAQARDALDLIEVDYDPLPAGVDPEAMTKPGAPQVHADVPNNI
                     GFKWIVAGGDADRAFAEASVTVKLRIVQQRLAPTAMEPRAAVASYNKGTGQLTLWCTS
                     QNPHIHRFLCSVILKLPEHRVRVIAPEVGGGFGSKIPVYADEALVGLASMDLGRPVKW
                     TEDRSENYKATIHGRDHVEYVEMCGTREGQITGLRTTVYAGLGAYASTAGPGIPTILH
                     GLIYSGPYVIPNIHGTVYGVYTSTTPVDAYRGAGRPEATYLLERLIDLYARDIGMDPV
                     TIRRKNFIPKDKFPYTVATGLTYDSGNYEGALDKALSLFDLKQFREDQAEGRKRGRYL
                     GVGIVSFVEICGSGPSQVTGSVGFGGGQYDSAIVRVFPTGTVRVYIGGKPHGQGEETT
                     FAQIVADEFGIPIENVEIVAGDTETTPQGWGTYGSRTTAVCGSAVKLAAQRVKEKAKK
                     IAAHLMEANEADLEWKDNQFAVRGSPGSGKAFAEVALMANVAWNMPAGVEPGLEAVAF
                     FDPTNFVYPFGTHICTVEVNADTGELKILRYVCVDDCGPHINPLIVEGQIHGGVIQGL
                     GEALQEIVVYDGDGQLVTGTMMDYAVPKASQMPHIESSYTVTPSPVNPLGVKGIGEAG
                     TIPSAAALVSAVCNALEPLGVRHIDKPLTPARVWAAIQNAKGGAS"
     gene            complement(3226..3771)
                     /locus_tag="VFB89_09510"
     CDS             complement(3226..3771)
                     /locus_tag="VFB89_09510"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZM27583.1"
                     /translation="MTHPVTLTVNGVAYPLEVEARALLVHVLRDTLNLTGTHVGCDTS
                     QCGACTVLIEGHAVKSCTVLAVQADGLPITTIEGLAPADQLHPLQTAFWEKHGLQCGF
                     CTPGIIMMATELLSTKPDPSEAEIRHALEGNLCRCTGYQNVVAAIQAAGATLRGRRQQ
                     DRPAEAAESRSAVEHAAPAGV"
     gene            3951..4023
                     /locus_tag="VFB89_09515"
     tRNA            3951..4023
                     /locus_tag="VFB89_09515"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3984..3986,aa:Asn,seq:gtt)
     gene            4069..4968
                     /locus_tag="VFB89_09520"
     CDS             4069..4968
                     /locus_tag="VFB89_09520"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZM27584.1"
                     /translation="MTAPSSIAFLGLGAMGLPMARNLVAAGFNMTVWNRTSARAREVP
                     GAHPAPTPAAACERAEFAVSMLADDGAVEHTVLGQSGALTRLAPGTIHIGCSTISVAF
                     CRRLVETHAAAKVGFVAAPVFGRPEAASAKQLWIVPGGSQADVGRCAPIFTALGQGVF
                     PLGSAPHASLTKLLGNFMIASSIEMLGESLAAAEKAGLEPVRFLEIVTGTLFGAPLFK
                     RYGQLVADTTFEPAGFHLPLGLKDMRLALEATEEIKSPLPLASLVRDRMLTALALGRE
                     RFDWSGFTSVIREAAGLPALRAR"
     gene            complement(4975..5619)
                     /locus_tag="VFB89_09525"
     CDS             complement(4975..5619)
                     /locus_tag="VFB89_09525"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HZM27585.1"
                     /translation="MLAFATILFDLDGTLIDSVQLILESYRHTLASHGIPPRTDEEWL
                     RGVGTPLAAQFAPWRDDQGTLEAMIATYREYNLANHDRMVREFPGVVETVRAVRAKGL
                     KTGLVTSKARSGALRGLRLVGLDDAMDVLVCADEVTNPKPHPEPVAKAVGLIGADPAT
                     TLFVGDSIHDLNSGRAAGVRTAAVLWGPFSRAHLAPGKPDYWLEHPRDLLPLIG"
     gene            complement(5653..>7061)
                     /locus_tag="VFB89_09530"
     CDS             complement(5653..>7061)
                     /locus_tag="VFB89_09530"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HZM27586.1"
                     /translation="SFWRAAASSPAALAASLDRYQRLLQHAGDAARTGRRLDRRLLRS
                     LTKHLDDQLLLWELLPLESTQSELALDDLPLLERLRMAARSATQAPDPKLSRLALLLA
                     DGRCSVVFTGARETVRYLRDRLNGGPTAWCTGERAGIGPHPAPRRAVLDWFRPSATRA
                     ISERLAGLAPRHLIATDVAAEGLDLHRAERIIHYDLPWTPARLEQREGRACRAGAPHE
                     HVEAVRFEPPPAVENRLRQLACLTGKRALPAAVGLDDAGRGLWRWRVDLAERFRSCPE
                     AFGTAELRRPPAGILAGFTILPWTGSEPLATWVLWWDRQRGWTEDPNVIDSMLANAAR
                     SSDNESAETQAPSRKSIDSAISRLAAPIRERIRQVGRSRWLDRPRSPNACAVIARLQA
                     LGRGAARRRDTATLGHLQNALRFVGGGHTAGEQLFITRLAAMADREFEQAVVRMPSAA
                     PAIDAFQCRLTGLVVFSP"
BASE COUNT         1158 a         2284 c         2360 g         1259 t
ORIGIN      
        1 cctacttggc ccgctccagg gcgtgggtga gagcgtctcg cgttgcgacg gtaacgaggt
       61 gccgccggta tgccgccggc gcgtgcacgt cgccctgaat ttccaggcct tcgggcgccg
      121 ctgccgcgac gcgcgagatc acgtccggct ctccttttgt gccgaccagc tgatcgacgg
      181 ccgagacgcg ccgagccaca tcggccagcc cggtgagcgc gaccacggca tgcttgacgg
      241 tcaggcggct ccgcgcgatt acggcggcgg cggcgaccag cgagtatcct gatgcagcct
      301 ggttgaagct cacgtatgcc gtgccggcgc cccggggcag cggaggcagc agcacctcga
      361 cgaacagctc gtcctcggcc aaggccgtgg agaacggccc ctgaaagaat tcgcgggcgg
      421 gtacacttcg ctttccccgc ttcgatcgaa ggttgaaggt cgcatccagg gcgaccatga
      481 tcgccggcat gtcggacgcg ggatcggcgt gagccaggcc gccgccgatc gtgcctcgat
      541 tgcgaacctg caggtcaccg ataccctcgg ttgcttcagc gatgagggga aatcgttcgc
      601 gtatgaggtc ggactcgagc acctcgcgat agctggttag cgcgccgatg cgcgccccgc
      661 gccgatgctc tgtgactccc ttgagctcgc tcaagcggcc gatatcgatg agtcggctcg
      721 gctgtgccag ccggaagcgc atcatcggaa tgagactgtg accgcccgcc agaaccttgg
      781 cgccaccgcc ctgagccagc gccgtgagcg cgtcccggag ggacgtcgcc cgggtgtatt
      841 cgaagctggc cgggatcatg acgcgcctcc ctttgcgttc tgtattgcgg cccagacccg
      901 agccggtgtg agcggcttgt cgatatggcg gacccccagc ggctcgagcg cgttgcatac
      961 cgcgctgacc agcgccgccg cggagggaat cgttccggct tcgccgatcc ccttcacccc
     1021 gagcggattt accggtgaag gcgtcacggt gtacgagctt tcgatgtgag gcatctgact
     1081 ggctttcggg acggcgtagt ccatcatggt gccggtgacc agctggccgt cgccgtcgta
     1141 taccacgatt tcctgcagag cctcgccgag accctggatc accccaccgt gaatttgccc
     1201 ttccacgatc agtgggttga tgtgcggacc acaatcatcc acgcacacgt agcgaagaat
     1261 cttgagttcg ccggtgtcgg cgttcacttc gaccgtgcag atgtgcgtcc cgaatggata
     1321 gacgaagttc gtagggtcaa agaacgccac ggcttcgagc ccgggttcca cgcctgccgg
     1381 catgttccag gcgacattcg ccatcagcgc gacttccgcg aaggccttac ctgagccggg
     1441 agaaccgcgc acggcaaact gattgtcctt ccattcgagg tccgcctcgt tcgcctccat
     1501 gaggtgcgcc gcgatcttct tggctttctc tttgactcgc tgcgccgcca gcttgaccgc
     1561 tgagccacag actgccgtcg tccggctgcc gtacgtgccc cagccctgcg gcgttgtctc
     1621 ggtgtcgccc gcaacgatct caacgttttc gatcggaatt ccgaactcgt cggcgacgat
     1681 ctgagcaaaa gtggtctcct cgccctggcc gtgcggcttg ccgccgatgt acacccgtac
     1741 cgtcccggtg ggaaacaccc gcacgatcgc cgagtcgtac tggccgccgc cgaatccgac
     1801 actgcccgtc acctgagacg gaccggatcc gcagatctcc acgaacgaga cgataccgac
     1861 gccgaggtag cgtccgcgct tccggccctc cgcctgatcc tcgcggaatt gtttcaggtc
     1921 gaagagggaa agcgccttgt cgagcgcgcc ttcgtagttg cccgagtcat aggtaagacc
     1981 ggtggcaaca gtgtagggga acttgtcctt cgggatgaag ttcttgcgcc ggatcgtgac
     2041 cgggtccatg ccgatgtcgc gggcgtagag atcgatcagg cgctcgagca gataggtcgc
     2101 ttccggccga cctgccccgc gataagcgtc caccggtgtg gtggaggtat aaaccccgta
     2161 aaccgtcccg tgaatgttcg gaatgacgta cggaccggag tagatcaggc cgtgcaggat
     2221 ggtgggaatc cccgggcccg ccgtcgaggc gtaggcaccg agtccggcat agacagtggt
     2281 gcgaaggccg gtgatctgcc cttcgcgtgt gccgcacatc tcgacgtact cgacatgatc
     2341 gcggccgtgg atggtcgcct tgtagttctc cgatcgatct tcagtccact tgaccggccg
     2401 tcccaggtcc atggacgcga gccccaccag cgcctcatcg gcgtacaccg ggatcttgct
     2461 gccgaaacca cctccgactt cgggcgcgat tacccgtacg cggtgctcgg gcaatttgag
     2521 tatcaccgag cagagaaaac ggtggatgtg cgggttctgg ctggtgcacc agagcgtgag
     2581 ctggcctgtt cccttgttat agctggccac ggcggctcgc ggctccatcg ccgttggggc
     2641 cagtcgctgc tggacgattc gcagcttcac cgtgaccgat gcttcggcaa aggcgcggtc
     2701 ggcgtcacca ccggccacga tccacttgaa cccgatgttg ttggggacgt cggcgtggac
     2761 ctgaggcgct cccggcttcg tcatggcttc gggatcgacg cccgcgggca gtgggtcgta
     2821 atcgacttcg atcaggtcga gcgcgtcgcg cgcctgcgcc cgagtgccgg cgaccaccag
     2881 agcgacgccg tcgcctacgt agcgcaccgt gtcgtacgcg agcagaggat gcgggggggt
     2941 cttgagatcg cagttgggag ggttccatgc gcagggcacc ggtacgactc gatccttgat
     3001 gtccgcgcca gtgtaaaccg ccagcacacc gggcgccttt cgtgcagccg agacgtcgat
     3061 tcgcgcgagc ttggcatgcg cgtaggggct acgcaggatt gcggcgaagg tcagccccgg
     3121 aagcttgacg tcatccgtgt aggtcgcggc ccccgtgatg agacgcggat cctcccgccg
     3181 cttgatcccg gaaccgaaga gcgtggtcgc catgtgactc tctccttaaa cgccggcagg
     3241 cgccgcgtgt tcgaccgcgc tgcgcgactc ggctgcttcg gcgggacggt cctgttgccg
     3301 ccttccgcgc agcgtagcgc cggcggcctg aatggcggcc acgacgttct gatacccggt
     3361 gcaccggcac agatttcctt ccagtgcgtg gcgaatctcg gcttcgcttg ggtcgggctt
     3421 ggtgctcagc agctcggtag ccatcatgat gatgccgggc gtgcagaatc cgcactggag
     3481 cccgtgcttt tcccagaacg cggtttgaag cggatggagc tggtccgcag gggcaagccc
     3541 ttcgatggtg gtgatcggca agccgtcggc ctgcacggca agcacggtgc aactcttcac
     3601 ggcatggcct tcgatcagca ccgtgcacgc gccgcactgg ctggtatcgc agccgacgtg
     3661 agtgccggtg agattgagcg tgtcgcgaag gacgtgcacc aaaagcgctc gcgcttcgac
     3721 ttcgagcggg taggccacgc cattgacggt gagagtgacc gggtgcgtca tgaggccgga
     3781 ctccttgcag ctgcgcgggt gggcccgaac tggaaggctt aggctagtcc ctggcagcga
     3841 cccggtcaag attcactgcg caagcgcgag gagaagagag cagcaccgca cttgaccggt
     3901 atgccgctga ctggtaacct gcttaccccg tttaggttcc gtaacttcat tggggtgtag
     3961 ctcaatcggc agagcgcccg gctgttaacc gggaggttgc tggttcgacc ccagccgccc
     4021 cagtccgccg tactcatccg ccaatcccct tcctctgaaa gcatcacgat gaccgctcct
     4081 tcttcgattg cctttctagg cctcggcgcc atggggcttc cgatggcgcg taatctcgtc
     4141 gcagcgggat tcaacatgac ggtgtggaat cgtacttcgg ccagggctcg tgaggttcct
     4201 ggcgcgcacc cggcgccaac gcctgctgcc gcatgcgagc gggccgagtt cgccgtctcg
     4261 atgctcgccg acgacggggc ggtggagcat acagtgctcg gccagagcgg cgcgctgacc
     4321 cgtctcgccc ccggcacgat ccacattggt tgtagcacga tttcggttgc gttctgccgt
     4381 cggctggtcg agacccacgc ggctgccaag gtcggctttg tcgccgcgcc ggtctttggc
     4441 aggccggagg ctgcctccgc gaagcagctc tggatcgtgc ccggcgggag ccaggctgac
     4501 gtcgggcgct gcgcaccgat cttcacggcc ttggggcagg gtgtgttccc gcttggctca
     4561 gcgccccacg cgagtctcac caagctgctg ggaaatttca tgattgcgtc cagcatcgaa
     4621 atgttgggcg agtcgctggc ggcggctgag aaggcagggc tcgagccggt gcgatttctt
     4681 gaaatcgtca ccggcacgct gttcggcgcg ccgctgttca agcgttacgg ccagcttgtg
     4741 gcggacacca ccttcgagcc ggcgggcttc cacctcccgc tcggcttgaa agacatgcgg
     4801 cttgcactcg aggcgaccga agaaatcaag agcccgcttc ccctcgcgag tctggtgcgt
     4861 gaccggatgc tcacggcgct ggcgctggga agagagcggt tcgactggtc gggatttact
     4921 tcagtgattc gcgaagcggc cggtctgccc gcgctgcgag ctcgatgacc gggctcagcc
     4981 gatcagcggg agaaggtctc tcggatgctc gagccaatag tccggctttc cgggcgcgag
     5041 atgcgctcgc gagaacggac cccagagaac cgcggccgtg cggacgccgg cggcgcgacc
     5101 ggagttgagg tcgtggatgc tgtcgcccac gaagagggtc gtcgccgggt ccgcaccgat
     5161 cagccccacc gcctttgcca ccggctccgg atgcggcttg ggattggtca cttcgtcggc
     5221 acagaccagg acgtccattg cgtcgtcgag acctaccagc cgaagccctc gtaacgcgcc
     5281 cgaccgcgct ttgctggtca cgagtccggt cttgagtccc ttggctcgca cggcgcggac
     5341 cgtctccacc actccgggaa actcccgcac catccggtcg tgattcgcca ggttgtactc
     5401 gcggtacgtc gcgatcatgg cctccagcgt gccctgatcg tcccgccacg gggcgaactg
     5461 cgcggccagc ggggttccga cgccgcgcag ccactcctcg tcggtgcgcg gcgggatccc
     5521 gtgactcgcg agcgtgtgcc gatagctctc gaggatcagt tgaactgaat cgatcagagt
     5581 cccgtcgaga tcgaaaagaa tggtcgcgaa ggctagcatg gcgggaaggt agcggcgagg
     5641 cgcagggagg gcctagggcg aaaacacgac cagtcccgtg aggcggcatt ggaacgcgtc
     5701 aattgccggc gcggcgctcg gcatccggac aacggcctgc tcgaattcgc ggtccgccat
     5761 ggccgcgagc cgggtaatga agagctgctc cccggcggta tggccgccgc ctacaaaacg
     5821 cagcgcgttc tggaggtggc ccagcgtcgc agtgtcacga cgtcgtgcgg ctccccggcc
     5881 gagcgcctgc aaccgggcaa tgactgcgca ggcgttgggc gagcgcggtc gatccagcca
     5941 tcgtgaccgg ccgacttgcc ggatccgttc gcggattggc gcggcgagcc gcgagatagc
     6001 agaatcgatc gacttgcggg aaggagcttg tgtctcggcc gactcgttgt cggacgatcg
     6061 cgcggcgttt gccagcatgc tgtcgatcac gtttgggtct tcagtccatc cccgctgccg
     6121 atcccaccag agtacccagg ttgcgagggg ctcgctcccc gtccatggga gaatcgtgaa
     6181 accggcgagg atgccggccg gcggcctccg cagctcagcc gtgccgaagg cctcggggca
     6241 cgagcgaaac cgctccgcca gatccacgcg ccatctccaa agccccctgc ctgcgtcgtc
     6301 caggcccaca gcggctggaa gcgcgcgctt gccggtgagg cacgcgagct ggcgaagccg
     6361 gttttcgact gccggaggcg gctcgaaccg cacggcttcg acgtgttcat gaggggcgcc
     6421 ggcccggcag gctcgccctt cccgttgctc gagccgggcg ggcgtccagg gaaggtcgta
     6481 atggatgatt cgctctgccc ggtgaagatc gaggccttcc gctgccacgt cggtcgcgat
     6541 caaatggcgg ggggccaggc cggcgagccg ctcgctgatc gcgcgcgtag ccgatggccg
     6601 aaaccagtcg agcacagctc gccgtggcgc gggatgcggc ccgataccgg ctcgctcgcc
     6661 ggtgcaccac gcggttggcc cgccattcag ccgatcgcgc aggtagcgga cggtctcgcg
     6721 tgcgccggtg aagaccaccg aacagcggcc gtcagccaaa agcagagcga gtcgggagag
     6781 cttggggtcg ggagcctgtg tggcggagcg agcggccatg cggagccgct cgagcagcgg
     6841 gaggtcgtcc agggcaagct cggactgggt cgactcgaga gggagaagct cccaaagaag
     6901 cagctgatcg tccaggtgct tcgtcagaga ccggagcaat cgacgatcca gccgccgccc
     6961 ggttctggca gcatcgcccg cgtgttggag aagccgctga taacgatcga gcgaagccgc
     7021 caatgcggcc gggctcgaag cggccgctcg ccagaacgag g
//
LOCUS       DASRJQ010000422         2079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_561425, whole genome shotgun sequence.
ACCESSION   DASRJQ010000422 DASRJQ010000000
VERSION     DASRJQ010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561425"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="VFB89_09535"
     CDS             complement(<1..748)
                     /locus_tag="VFB89_09535"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HZM27587.1"
                     /translation="MTNPELDLAKLRIDRDAPPAVKRAFARTLTLAAGAVVLIGGVVV
                     FARRGGAVPVQVVVAAAANGGSGGGPALGTSVTANGYVVARTRASVSAKVAGRLAFLG
                     VSEGSRVSKGEVIARLENADYLAQVAQAQAAVASARADSIEAAADRDQMEREAQRLRT
                     IHAQNRELVAQQEVDQSETRAAQSAARAQSAAARLVAAAAGLRYASAAFDNTVIRAPF
                     SGTVLRKEAEVGEVVAPSVGGGLTRGAVVTM"
     gene            complement(857..1639)
                     /locus_tag="VFB89_09540"
     CDS             complement(857..1639)
                     /locus_tag="VFB89_09540"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HZM27588.1"
                     /translation="MRRPTAWVRRNPLFRTVASLCRQYLKWYGNASYKPEKNGERWLL
                     EQLGSQGFTTILDVGANVGKWTTLAARVFPAASIYALEIVPATFERLRAATAQLPRVK
                     ALNLGLAERTGTLSIRYHPSASAHATFTEYPHGWTGETIDCPVTTGDELLAAHQLTSV
                     DFLKLDIEGAEHLALRGFAHTLTERRIRFVQFEYGRVNILTRFLLKDFYELFERAGYA
                     VGKIYPDYVDFRTYELSDEDFLGPNYLACRADEPLLSRLRGA"
     gene            complement(1636..>2079)
                     /locus_tag="VFB89_09545"
     CDS             complement(1636..>2079)
                     /locus_tag="VFB89_09545"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HZM27589.1"
                     /translation="WLGKGIAGDDTHGHVDDLCRFVNPTTVVTCSEHHFSDENYRALS
                     ENRERLAGMQTEDGSKLEVVELPMPEPLVFEGRRLPASYANFYIGNAAVLVPTFNDPA
                     DRQALGILSEVFDRPVVGIHAVDLVWGLGTLHCLTQQEPEPGLEK"
BASE COUNT          333 a          694 c          703 g          349 t
ORIGIN      
        1 ccatcgtcac caccgcgccg cgcgtcagcc cgcccccgac cgagggcgcc acgacctcgc
       61 ccacttccgc ttccttgcgc agcacggtgc cgctgaaggg cgcgcggatc acggtattgt
      121 cgaacgcggc gctggcgtag cgcagccccg ccgccgcggc cactaatcgg gcggccgcag
      181 attgggcccg cgccgccgac tgcgcagccc gcgtttcgga ctgatcgact tcttgctggg
      241 ccaccagctc gcggttctgg gcgtggatcg tccgcaggcg ctgggcctcg cgctccatct
      301 ggtcccggtc ggccgccgct tcgatggagt cggcccgcgc tgaggctacg gcggcctggg
      361 cctgagcgac ctgggccagg tagtcggcgt tctcaagccg ggcgatgacc tcgcccttgc
      421 tcacgcgcga cccttcgctg acgccgagaa acgcgagacg acccgcgacc ttggcggata
      481 cggacgcgcg ggtacgggcc accacgtagc cgttggccgt cacgctcgtc ccaagcgcgg
      541 gcccgcctcc ggatcctcca ttcgccgccg cagcgacaac gacttgcacc gggactgcgc
      601 ccccgcgccg cgcgaacacc accacccccc cgatcaggac gaccgcaccg gccgccaacg
      661 tcaaggtacg ggcgaacgcc cgcttgaccg cgggaggggc atcacggtcg atgcgcagct
      721 ttgcaagatc gagctcgggg ttcgtcatga ttcgcgcgaa aactaatcgg gcattcgtgc
      781 gctggtagct acctcgccgg cgtggggcat gttccggcca cgggaagtgg tctgctggct
      841 ctccgatgca gtggccttag gcgccgcgca atcgcgaaag caggggctcg tcggcccggc
      901 aggcgagata gttcggtccg aggaaatcct catcggagag ctcataagtc ctgaaatcca
      961 cgtagtcggg atagatcttc ccgactgcgt accccgcgcg ctcaaacagc tcgtaaaagt
     1021 ccttgagcag gaaccgggtg aggatgttga cccggccgta ctcgaactgc acgaagcgaa
     1081 tccgccgctc ggtcagggta tgggcgaacc cccggagggc caggtgctcg gctccttcga
     1141 tgtcgagctt caggaaatcc acgctggtga gctggtgcgc ggcgagcagc tcgtcacccg
     1201 tggtgacggg acagtcgatt gtttcgcccg tccagccgtg ggggtattcg gtgaacgtgg
     1261 cgtgcgcgct ggcggacgga tgatagcgga tggagagggt accggtccgc tccgcaagtc
     1321 cgagattgag ggccttgacc cgggggagct gcgctgtggc ggcgcgaagc cgctcaaagg
     1381 tggcgggtac gatctcgagc gcgtagatcg aggcggccgg gaacacgcgc gcggcgagcg
     1441 tggtccactt cccgacattg gcccccacgt cgaggatcgt cgtgaacccc tgactcccga
     1501 gctgctcgag cagccaccgc tccccgtttt tttcgggttt gtagctcgca ttgccgtacc
     1561 acttcaggta ctggcgacac aggctcgcca cagtccgaaa cagcgggttt cggcgaaccc
     1621 aggccgttgg tcgtctcatt tttcgaggcc gggttcgggc tcctgctgcg tcaggcagtg
     1681 cagggtaccc agcccccata cgagatccac ggcgtggatg ccgacgaccg gccggtcgaa
     1741 cacttcgctc aggattccca gggcttggcg gtcggcgggg tcgttgaacg tggggaccag
     1801 gaccgcggca ttgccgatgt agaagttggc gtagctcgcc gggaggcggc ggccttcgaa
     1861 gacgagcggc tcgggcatcg gcagctccac cacctccagc ttggagccat cctcggtttg
     1921 catcccggcg agacgctcgc ggttctcgga cagggcgcga tagttctcgt cgctgaagtg
     1981 gtgctcggag cacgtcacga cggtggtcgg gttcacgaaa cggcaaagat catccacatg
     2041 gccatgcgtg tcgtcgccgg cgatgccctt gccgagcca
//
LOCUS       DASRJQ010000423         2175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1478251, whole genome shotgun sequence.
ACCESSION   DASRJQ010000423 DASRJQ010000000
VERSION     DASRJQ010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478251"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VFB89_09550"
     CDS             complement(<1..482)
                     /locus_tag="VFB89_09550"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZM27590.1"
                     /translation="MLDAILKPPGDGAASMAPALLAGEWLGDGSAERVGPYVRRVVSR
                     ARLAGPGDPARAADYACVAKRHMARLAPAARAEILERAAGIAVERRDDFARLIALELG
                     KPIKDGRGEMDRVGDTLAVCAAEARQIGGELLPVAGWRRGIGNTAMTYRAPAGVALAI
                     T"
     gene            696..1508
                     /locus_tag="VFB89_09555"
     CDS             696..1508
                     /locus_tag="VFB89_09555"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZM27591.1"
                     /translation="MKAATGRKLPAKQVTAEEPVDQHGVRSVQRALDILSLLTDDRPA
                     VSIREIVDSTGLAKTTVLRLVSTLEQSGLLWATASGYMAGPGLWRWAHLARRSWELPP
                     ETQRSMRELAAHHRETVNMYVARDVHRVCIAQQESPQPLRHVVHVGDELPMWAGASAK
                     VLLRDASAGLLERIARGSPYGPGHTKRLREWIDDAAVRGYAVSHGEREDGLSAVAAPI
                     LGRSGRVVAALGLSGPTVRFTESRIAEFAADLKRIAQQMSERGFDHPLATST"
     gene            1566..>2175
                     /locus_tag="VFB89_09560"
     CDS             1566..>2175
                     /locus_tag="VFB89_09560"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZM27592.1"
                     /translation="MTKRPLAGVRVLDLTNVLAGPYCTYQLALLGAEVLKVEIPGSGD
                     LARHLGADPDLNEVGLGASFLAQNAGKQSVELDLKTDADRKVFIELVRAADVLVENFR
                     AGVLGRLGFDWPALRSLNPGLIYCAISGFGQTGPMSQAPAYDQVIQGLSGIMSITGTP
                     ESAPLRVGFPVCDAVGGLTAALAVVSAIAGRNNDGQGCFLDVS"
BASE COUNT          366 a          712 c          734 g          363 t
ORIGIN      
        1 gtgatggcca gcgccactcc agccggtgcc cggtaggtca tcgccgtgtt gccgatgccg
       61 cgtcgccagc cggcaacagg cagcagctcg ccaccgatct ggcgcgcctc ggcggcacag
      121 accgcaagtg tgtcaccgac gcgatccatc tccccgcgcc cgtccttgat tggcttgccc
      181 agctcgaggg caatgagacg ggcgaagtcg tcacggcgct caactgcgat gcccgctgcg
      241 cgctcgagaa tttccgcacg cgcagcgggc gcgaggcgcg ccatgtgccg cttcgcgacg
      301 catgcgtaat cggccgccct cgccggatcg ccaggtccgg cgagcctggc ccggctgacc
      361 acgcgccgca cgtacggccc aacccgctcc gccgatccgt cgccgagcca ctcccccgct
      421 agcagcgctg gagccataga agcagcgcca tcgccgggtg gcttgaggat cgcatcgagc
      481 attgtgaggg gccttcctgg aaccgctgtg cggacctact agaccactgt gcggactgga
      541 tgttaacgta cgggcgcccg cctgttctgg caagaggccg agtccagaac gttacggacg
      601 tatgaagacg ccgcccaggc gagagaatcg aggcggcacc ggtcgcgatc gtgcgttcgt
      661 gaccacctac cggaccagac ggagagtgag gaaccgtgaa ggcggcaacc ggccggaagc
      721 ttccagccaa gcaagtgacg gccgaggagc ccgtcgacca gcacggagtt cgcagcgttc
      781 agcgcgcgtt ggacatcctt tccctcctga ccgacgatcg gcccgccgtg tcgattcgcg
      841 agatcgttga ttcgacgggg ctcgcgaaga ccaccgttct gcgactggtt tcgacgttgg
      901 aacagagcgg tctgttgtgg gcgaccgcga gcgggtacat ggctgggccg gggttgtggc
      961 gctgggcgca tctcgcccgg cgaagctggg aactgccccc cgaaacgcag cggagcatgc
     1021 gagagctggc ggcacatcac cgtgagacgg tcaacatgta cgtggcgcgc gatgtccatc
     1081 gcgtgtgcat cgcacagcag gagagccctc agccactccg ccatgttgtg cacgtcgggg
     1141 atgagttgcc catgtgggcc ggcgcctccg cgaaggttct ccttcgagat gcgagcgctg
     1201 gtctgctgga aagaatcgcg cgcggctcgc cctatggccc tggacacacc aagcgcctca
     1261 gggagtggat cgatgatgcc gcagtccgcg ggtacgccgt gagtcatggc gaacgggagg
     1321 acggtctctc ggctgtcgcc gcgccgatcc tgggccgctc cggccgagtc gtcgcggccc
     1381 tcggactgag cggtccaaca gtccgattca ccgaaagccg gatcgccgag ttcgccgcag
     1441 atctcaaacg gatcgcccag cagatgtccg agcgcgggtt cgaccatcca ctcgcgacct
     1501 cgacctgagt cgtcggctgg cgagatgccc gccgacccgc catgacgctc acgaggagac
     1561 gccgcatgac gaaacgaccg ctcgccgggg tccgggtgct cgatctgact aacgtcctcg
     1621 cggggccgta ctgcacctat cagctggcac tgcttggtgc cgaggttctg aaggtggaga
     1681 tcccgggcag cggggacctc gcccggcacc tgggagcgga tcccgacctc aacgaggttg
     1741 ggctgggcgc atcgtttctg gcccagaatg ccggcaaaca gtcggtcgaa ctcgatctca
     1801 agaccgacgc cgatcggaag gttttcatcg agttggtgcg cgccgccgac gtgttggtcg
     1861 agaactttcg cgcgggtgtg cttggccggc tgggattcga ctggccggcg ctgcggtctc
     1921 tgaatcctgg cctcatctac tgtgccatca gcgggtttgg gcagaccggc ccgatgagtc
     1981 aagcaccggc ctacgaccag gtcatccagg gcctgtccgg aattatgagc atcaccggta
     2041 cccccgaaag tgcgccgttg cgggtaggtt tcccggtttg cgatgcggtg ggcgggctga
     2101 ccgcagcgct cgcggtggtc agtgcgatcg ctggacgcaa caacgacggc cagggttgct
     2161 tcctcgatgt gtcca
//
LOCUS       DASRJQ010000424         9148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1723977, whole genome shotgun sequence.
ACCESSION   DASRJQ010000424 DASRJQ010000000
VERSION     DASRJQ010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 9148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9148
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1723977"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VFB89_09565"
     CDS             <1..549
                     /locus_tag="VFB89_09565"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZM27593.1"
                     /translation="YRTAREATGAAKAQYELVRSGVRAEDRAAAIADLRKAEGAVAEA
                     TSYVDDNTLRSAVDGQVMVKVVEQGELVSPGLPVLTVVDLSDQWITVNLREDRLAGLK
                     VGDTLTAVIPALGSEPFRFAVYYISPLGSFATWRATRESGGFDVRTFEVRARPPRPID
                     GLRPGMSALIDWPRERTARVSG"
     gene            546..1715
                     /locus_tag="VFB89_09570"
     CDS             546..1715
                     /locus_tag="VFB89_09570"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM27594.1"
                     /translation="MSRIARVIVRELRRLPHFPVLWALLGPIPLAAGLLLTGVFQAEV
                     ARRLPIGILDFDATQTARLAARWVGAARTVNIVARVQDLGEAQALLVQRKVYAVLVVP
                     RHFERDLLHGRSPRVTLLYNEQYLTAGNLISADVARAVNAGSGVVAFELQRSRGVTQA
                     AAEAAAEPVRVDSRLLFNPAINYARGVGLVLVVGLIQIVLGLSTIYVVGRELRDSTAG
                     EWLAAAGGSPTVAWIGKLAPYLIYDLVLMLGLVGGYMAWFHIPVRGSLGLLALGAVAF
                     SLASRTIGVLLTVWLANLRKALGVGSVFFGTAVAFSGVTFPLIAMSTFAKVWSGCVPL
                     TRFMLLLRDQVMVGAPLRISAAPILVLAATALVAGLVALPRMGRVLRDSRLWGQE"
     gene            1712..2905
                     /locus_tag="VFB89_09575"
     CDS             1712..2905
                     /locus_tag="VFB89_09575"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM27595.1"
                     /translation="MKALARCFRQTLRRMFREQETLVLLFGASVLYALFYPTPYMRQV
                     LRDLPIVVVDRDHTALSRQLTRWLDASEAARVTSRITDLNAAQDSVRAGVAGAVLLIP
                     QDFERHVLRREPAYVEAYADASYFLVYSTALRAINGVVGTLSTGIAVRRLEASGFTEK
                     AALERTRPVTFVSWPLFNPLAGYGTFVVPAVFILILQQTLLIGIGALRVAERREDQSG
                     REPLWAILGGKLGATALIYVGHALFMFGVVFSLYGFPMRASWLDVFIFLVPYFAAVTL
                     LGLAVAELFDRPESSTVALAAMSLPALFMSGLSFPAEVQAGWVRLLAVVLPSTFGIQG
                     FLQLAEMGARLDQTLRPWAALWAQVVVYWTLAWLVLRYRGRALYSRQLSVVRPNEGVP
                     GPKTI"
     gene            2999..3133
                     /locus_tag="VFB89_09580"
     CDS             2999..3133
                     /locus_tag="VFB89_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27596.1"
                     /translation="MRNSHLLAEHLPNAMLLTYPDAGHGSLFQFHDSFVRQTMLFLDS
                     "
     gene            3130..3591
                     /locus_tag="VFB89_09585"
     CDS             3130..3591
                     /locus_tag="VFB89_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZM27597.1"
                     /translation="MSEEHAGQVRAHQPGQAVFRSVLPEDIDWKPFPAFPPSVRLAVV
                     VGQPSEPGPYVIRVKVPSGVKLPPHRHPEDRVYTVISGVFYIGRGDRFDADKLEAYPP
                     GSVIVLPGYTSHFHWAKSGEYITQVTAIGPLGLEYVDPGDDPRELGHGQRS"
     gene            complement(3694..4299)
                     /locus_tag="VFB89_09590"
     CDS             complement(3694..4299)
                     /locus_tag="VFB89_09590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HZM27598.1"
                     /translation="MSVGGYTGTGLRLVGFAGSLRRDSFNRALLVAAQQVAPAGMAIE
                     LVQIGQLPFYNADVEKEGDPPSVAKFKADLRQADGLLIATPEYNAGIPGVLTNAIDWA
                     SRLPGRAPLTGKPVALMGASPGQIGTARAQLHLRQLLAHVHARTLPPPELLVAKAHEK
                     FDSHHRLTDEHTRGVLITLLERFARWIHREQAAIAADRLGS"
     gene            complement(4307..4516)
                     /locus_tag="VFB89_09595"
     CDS             complement(4307..4516)
                     /locus_tag="VFB89_09595"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZM27599.1"
                     /translation="MIEYPQHWTCEVIVLRLEGYLLRTIPLGEALAVAEHLEACDWCA
                     QLVTLKVDQGLRPGGPEFPVEEPGG"
     gene            5007..5672
                     /locus_tag="VFB89_09600"
     CDS             5007..5672
                     /locus_tag="VFB89_09600"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZM27600.1"
                     /translation="MGVVPLQDVSRRPAHFPVVTAGLVLINVLVFVVELWGGEPFVTR
                     WSVIPADIVAGHNWITILTSMFMHGSWSHLLGNMVFFWAFGPEVEDLMGRSRYLIFYL
                     LGGLAATLAQLAAGPTSTVPNLGASGAIAAVMGAFLVTYPRDSIRSLLMIFVFVRVAY
                     IPAALLIGLWFLSQLLNLGAVARLPEQSGGVAYAAHVGGFIYGTVVTRLFLGRRGLPA
                     PST"
     gene            5764..6624
                     /locus_tag="VFB89_09605"
     CDS             5764..6624
                     /locus_tag="VFB89_09605"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF027552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZM27601.1"
                     /translation="MADAIAIGRAQRCPLLNYSDSSGRMTAQARRFAAFVVITSLTLA
                     FQSTAACIGGAGARSSLRILFIGNSYTSVNDLPNVFAQLAKSGGHAVENGVAAPGGWT
                     LFAHARSAETLNKLKSSKWDVVVLQEQSQVPASEQARTQAMYPAVRLLVRQIEEGGAT
                     PILFLTWAHREGWPESGLIGYEATQARITEGYLRIAQELGVSVAPVGDAWLMATKEHP
                     DLELWQGDGSHPTEQGSYLAACVFYATIFRQRPEGLSYRGRLPKKAASVLQSTAANVV
                     LKDPKRWNLR"
     gene            6711..7202
                     /locus_tag="VFB89_09610"
     CDS             6711..7202
                     /locus_tag="VFB89_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27602.1"
                     /translation="MRIALCIACGLFGLLPADRAQAQTKLAAKMACAKPDPNYTAPVG
                     DEANHVLTLVSQKCTWSQGDLGGDKLKDETDTFTSDVNGNLSHDRGYGAGSVASGDKY
                     VVKFKGTTTLKGEKPVSADCTWTFKSGTGKLKGLTGKGTCKGTFSADGTSTWDIQGEY
                     KIS"
     gene            complement(7227..7730)
                     /locus_tag="VFB89_09615"
     CDS             complement(7227..7730)
                     /locus_tag="VFB89_09615"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZM27603.1"
                     /translation="MALIDGMLQELEDEARSTRRILERVPDNRLDWRPHNKSRSLGQL
                     AWHIAIVPGAVAEFVASPSPAQAPEFGPDPTPAKSAELIPALDQSVAKAKTVLGGMDD
                     TTLLATWRMMSGDRELLAVPRMAMLRSIMLNHWYHHRGQLTVYLRELGVPLPSIYGPT
                     ADENPFG"
     gene            complement(7740..8189)
                     /locus_tag="VFB89_09620"
     CDS             complement(7740..8189)
                     /locus_tag="VFB89_09620"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HZM27604.1"
                     /translation="MTAIAPGIDNLSLSIAEEVHINAPIATTFAALLEEIGPSNKGHN
                     DTPMPMKLEAWPGGRWFRDLGDNNGHLWGHVQAIKRPTLLEINGPLMMSFGVASNVQY
                     RLKEENGGTTITFHHSALGLFPEGYREALGQGWPLLVERIRRRAEKK"
     gene            complement(8161..8523)
                     /locus_tag="VFB89_09625"
     CDS             complement(8161..8523)
                     /locus_tag="VFB89_09625"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZM27605.1"
                     /translation="MPRAATTADSFNAVAEPRRRDILDFLAPAERPVGDIVESLGLAQ
                     PSVSKHLRVLLEVGLVEVRREGRHAFYRTNAEAIKPVHEWTSRFERYWRDQLSRVKER
                     AEQATKGEQHDRDRPRHR"
     gene            8714..>9148
                     /locus_tag="VFB89_09630"
     CDS             8714..>9148
                     /locus_tag="VFB89_09630"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZM27606.1"
                     /translation="MDLGLSGQVAVVTGASKGIGLAVTKALVGEGVRVVAGARNVGGE
                     LGALAQGGAVRALELDLSTRDGPQKLVDGAADYGRLDILVNNVGAVKLRLEGLLSVSD
                     DDWLASLNLNLMSAVRATRAALPVMLKSGRGSIVTVSSVNSFL"
BASE COUNT         1484 a         2900 c         3037 g         1727 t
ORIGIN      
        1 tatcgcacgg cgcgggaagc gaccggagcc gccaaagccc aatacgagct ggtgcggtcc
       61 ggggtgcgag ccgaggaccg tgccgccgcg atcgccgacc tccgaaaggc ggaaggggcg
      121 gtggccgagg cgaccagtta cgtcgacgac aataccctcc ggtcggcggt tgacggccag
      181 gtgatggtca aggtggtcga gcagggcgag ctggtctcgc ccggcttgcc ggtgctcacg
      241 gtcgtggatc tctcggacca gtggatcacg gtgaatctgc gggaggaccg gctggccggc
      301 ctcaaggtcg gcgatactct gacggcagtg atcccggcgc tcggatcgga gcccttccgc
      361 tttgcggtct actacatctc cccgctgggc tcgtttgcca cctggcgggc gactcgcgaa
      421 agcggtgggt tcgacgtccg gacgttcgaa gtgagagcgc gtcctccccg gcccatcgac
      481 ggacttcgac ccggcatgag tgcgctgatc gactggccgc gagagcgcac ggccagggtt
      541 tcgggatgag ccgcatcgct cgcgtgatcg tccgcgagct ccgccgcctg ccgcatttcc
      601 cggtgctctg ggccctgctg ggaccgatcc cgctcgcggc cggcctgttg ctcacgggcg
      661 tctttcaggc ggaggtggcg cgccggctcc ccatcggcat ccttgacttc gacgcgaccc
      721 agaccgcacg actggcggcc cggtgggtcg gcgccgcgag gaccgtgaac atcgtcgcac
      781 gagtccagga tctgggggag gcgcaggccc tcctggtcca acgcaaagtg tacgcggtgc
      841 tcgtggtgcc tcgccacttc gagcgggatc tgctgcatgg ccgctcgcct cgggtgacac
      901 tgctgtacaa cgagcaatat ctgacggcgg gcaacttgat ctccgccgat gtcgcgcgcg
      961 cggtcaacgc cgggtcgggc gtggtagcct tcgagctgca gcgtagcaga ggggtcaccc
     1021 aggccgcggc ggaagcggcg gcggagcccg tccgggtgga ctcacggctg ctgttcaatc
     1081 ctgcgatcaa ctacgcccgc ggcgtggggc tggtcctggt cgtcggtctg atacagatcg
     1141 tgctcgggct ctccacgatc tacgtggtcg ggcgagagct cagggattca accgctgggg
     1201 agtggctggc cgcggcgggc ggctcaccga cggtggcttg gatcggcaag ctggctccct
     1261 acctgatcta cgatctggtg ctgatgctgg ggctggtcgg cggctacatg gcctggtttc
     1321 acatccccgt gcgtggcagc ctcggactgc tcgcgctggg tgccgtggcg ttctccctgg
     1381 ccagcaggac catcggggtc ctgctcacgg tctggctcgc gaacctccgg aaagccctcg
     1441 gggtgggctc cgtgttcttc gggactgcgg tcgccttcag cggggtgacc ttccccctga
     1501 tcgcgatgtc caccttcgcc aaggtgtgga gcgggtgcgt gccgctcacc cgtttcatgc
     1561 tcctcttgcg cgaccaggtc atggtgggcg caccactccg catttcggcg gccccgatcc
     1621 tggtgctggc ggcaacagcg ctggtcgccg gcctcgtcgc gttgccccga atgggccggg
     1681 tgctgcggga ttcgcggctc tggggccagg aatgaaggcc ctggctcgct gcttccgcca
     1741 gaccctccgg cggatgtttc gcgagcagga gacgctcgtc cttctgttcg gtgccagcgt
     1801 gctgtatgcg ctgttctatc cgacgcccta tatgcggcaa gtgctgcgtg acctgcccat
     1861 tgtcgtggtc gaccgggacc acaccgcctt gagccgccag ctcacgcgtt ggctcgatgc
     1921 cagcgaggcc gcgcgcgtga ccagccggat caccgacctc aacgccgccc aggactcggt
     1981 gcgagccgga gtcgcaggcg cggtccttct catcccccag gacttcgagc gccatgtgct
     2041 gcgccgcgag ccggcctatg tagaagccta cgccgacgcc agctacttcc tggtctacag
     2101 cacggcactc cgggccatca acggcgtcgt gggcaccctc tcgaccggca ttgcggtgcg
     2161 gcggctcgag gccagtggat tcaccgagaa ggccgccctc gagcgcaccc gcccggtcac
     2221 ctttgtctcc tggccgctct tcaacccgct cgccggctac ggcaccttcg tggtgccggc
     2281 cgtcttcatc ctgatcctgc agcagacctt gctgatcggc attggcgccc tgcgggtggc
     2341 cgagcgccgg gaggaccaat cgggcaggga gccactctgg gccatcctcg gcgggaagct
     2401 cggcgcgacc gcgctcatct atgtcgggca cgcgctcttc atgtttggcg tggtgttcag
     2461 tctctatggc ttccccatgc gagcctcgtg gctcgacgtc ttcatcttcc tggtgccgta
     2521 cttcgccgcg gtgacgctgc tgggcctcgc ggtcgcggag ctgttcgatc ggcccgaatc
     2581 ctcgaccgtg gcactcgcag cgatgtcgct gccggcgctc ttcatgtcgg gcctcagctt
     2641 tccagccgag gtgcaggccg gatgggtccg cttgctggcg gtggtgctgc ccagcacgtt
     2701 tgggattcag gggttcctgc agctggccga gatgggcgcc cgcctggatc agaccctgcg
     2761 cccctgggcg gcgctctggg cccaggtggt cgtgtattgg accctggcct ggttggtgct
     2821 ccgatataga ggaagggcgc tgtactcccg ccagctgagt gtggtgagac ctaatgaggg
     2881 ggtgccggga cccaagacga tctgacgtcg tgcggtggag gagccgcggt tgatttgccc
     2941 gttcgacagc tgttgacatg aagccgcagt gaacggggtc cgggacatga taatttcggt
     3001 gagaaactcg cacctgttgg ccgagcacct gcccaacgcg atgctgttga cctatcccga
     3061 cgcgggccat ggctcgctct tccagttcca tgactccttc gtgcggcaga cgatgctgtt
     3121 cctggactca tgagcgaaga acacgccggt caagttcgag cccatcagcc cggccaggcc
     3181 gttttcagat cggtgctgcc ggaagacatc gactggaagc cattccctgc cttcccgcct
     3241 tcagttcggc tcgcggtcgt tgtcggtcag ccttcggagc ctgggcccta cgtgatcagg
     3301 gtcaaagtac cctccggtgt gaagctgccc ccacacagac atcctgagga cagagtctac
     3361 accgtgatct cgggcgtctt ctacattggc cggggcgacc ggttcgacgc cgacaagctg
     3421 gaggcgtatc cccccggcag cgtcatcgtg ctgccaggct atacatccca cttccactgg
     3481 gcaaaatcag gtgagtacat cacacaggta accgccattg gtccgttggg gctcgaatac
     3541 gtggatcccg gcgacgatcc gcgtgagctc gggcatggcc agaggagcta gcggacgccg
     3601 acccctgatg ggtagcggtc ggtcgttaac gttgtcgcag cgcagccgta ggcctggtcc
     3661 cgacttcggg tcatagagag gcgagatgcg tagtcaggac cccaaccgat ctgcggcaat
     3721 agcggcctgt tcgcggtgaa tccagcgagc gaatcgttcg agcagagtga tcagtacgcc
     3781 gcgggtatgc tcgtcggtga gccggtggtg cgaatcaaac ttctcgtgtg ccttcgccac
     3841 aaggagctca ggcggcggga gggtccgggc gtggacgtgg gcaagaagct ggcggaggtg
     3901 gagctgtgcc cgtgccgtgc cgatctggcc cggggatgca cccatgagcg caactggctt
     3961 gccggtcagc ggcgcacggc ccggcagtcg cgatgcccag tcgatcgcgt tggtgagaac
     4021 ccccggaatg ccagcgttgt actccggcgt cgcgatgagg agcccatcgg cctgccggag
     4081 gtccgccttg aacttcgcga cgctcggcgg atcgccttcc ttctcgacgt ccgcgttgta
     4141 aaaggggagc tgaccgatct ggaccagttc gatcgccatg ccggctggcg caacctgctg
     4201 ggccgccaca agcagggcgc ggttgaagga gtcgcggcgc aagcttccag cgaatccaac
     4261 aaggcgaagc ccggtgcccg tgtagcctcc aacgctcatc gaaggctcac ccaccaggct
     4321 cttcaaccgg gaactccggg ccacccggcc tcagcccctg gtccaccttg agggtcacca
     4381 gctgggcaca ccaatcgcac gcctcgaggt gctcggctac cgccaacgcc tccccgaggg
     4441 gaatcgtgcg gagaaggtag ccctccaggc ggaggacgat aacctcgcaa gtccaatgct
     4501 gcggatattc gatcatcaac actccgcgcg cccgcaacga ctgagcctca tgaatctacc
     4561 tcgaaggtcg cgaaaggccg caagccgccc gcgatctccg gggggaactc aactgtggtt
     4621 gtagccggtg cccgttggag cggcccttct ttgtattccc ggcgccgtca cacaatgtta
     4681 catacggagg ctagccgtag gtcgccccgc gcgtagaata gaccacctcc gatcccaaca
     4741 gcctctcatc tccttcgtcc gaggccagga ggagcatcag gatggtcgcg ctcgatcgca
     4801 agcccgctca cagcgctctc atccgggaac tcgcctccaa cgtcgaccgc gcgatagcgg
     4861 gcgaacggca gcacgtgtgt ctccgagaga gaagagggga acctgcgtgc gcgtgctatc
     4921 gagatgcgca ccatgggggt tggcatcggt gcttgtgcgg ggcggaatgg cggagctcat
     4981 tgtcctggac tacctgaggg cgtgatatgg gagtggttcc gctgcaggat gtttctcgcc
     5041 gcccggctca tttcccggtt gtcactgccg ggctcgtcct catcaatgtg ttggtgtttg
     5101 tcgtcgagct gtggggcggc gagcccttcg taaccaggtg gtcagtgatt cccgccgaca
     5161 tcgttgccgg ccacaactgg atcacgattc tgacgtcaat gttcatgcac ggcagctggt
     5221 cgcacctctt gggcaacatg gttttcttct gggcgttcgg acccgaggtc gaagacctga
     5281 tgggtcggtc gcgatacctg atcttctacc tgcttggcgg attggccgcc accctagcac
     5341 agttggcggc cgggcctact tccactgttc caaacctcgg cgcgagtggc gcgattgcgg
     5401 ccgttatggg ggcattcctg gtcacctatc ctcgtgacag catccgctcg cttctcatga
     5461 ttttcgtgtt tgtgcgtgtc gcgtacatcc cggcagcgct cttgatcggc ctatggtttc
     5521 tcagccagct cctgaacctc ggcgcagtgg cgaggttgcc ggagcagagc ggaggagtgg
     5581 cctacgcggc ccatgtcggt ggcttcatct acggaaccgt cgtgacccgt ctctttctgg
     5641 ggcggcgggg gctacccgcg cccagcacct gatcctccga accttcttcc ttgacgcccg
     5701 ccgccgccga ctaaccgctc tcgccccaca cgcggcggcg ctgtatcctg agttcaggat
     5761 cccgtggccg acgcgatcgc gattggtcgg gcccagcgat gccccctact caattattcc
     5821 gatagctccg gccgcatgac ggcccaggca aggcgctttg cggcgttcgt cgtcatcacc
     5881 tctctgacgc tggcattcca gtcaacagcc gcctgtatcg gtggggccgg agcaaggtcg
     5941 tccttacgca ttctgttcat cggcaacagc tacacctccg tcaacgattt gcccaatgtg
     6001 tttgcccagc tggccaagag tggggggcac gcggtagaga atggagtggc tgctccaggc
     6061 ggctggacct tattcgcaca tgcccgctcg gcggagaccc tgaacaagct gaaatcatcc
     6121 aagtgggatg tcgtggtctt gcaagagcaa agccaggttc cagcaagcga gcaggctcgt
     6181 acccaggcga tgtaccccgc cgtccgcttg ctcgtccgcc agatcgagga gggcggtgcc
     6241 acgccgatcc tcttcctgac ctgggcccac cgcgagggat ggcccgaaag tggtctgatc
     6301 gggtatgaag ccacgcaggc ccggatcacc gagggttatc tgcggatcgc gcaggaactg
     6361 ggggtgtcgg tcgctccggt cggcgacgcc tggctgatgg cgaccaagga acatcccgat
     6421 ctggagttgt ggcagggcga cggcagtcat cccaccgagc agggaagcta tctggcagcg
     6481 tgtgttttct atgcaacgat tttccggcaa aggccggaag ggttgtcgta ccgcggccgc
     6541 ctgcccaaga aagcggcatc ggtcttacag agcaccgctg ccaatgttgt tctcaaagac
     6601 ccgaagcgat ggaatcttcg ctaagtcgcg ctgcctactg gcgaaacccg gccgcggcga
     6661 tacacttgcc gagtaaccct cagccctcac ctttcgaacg gaggcgcgcc atgagaatcg
     6721 cgctgtgtat cgcgtgcggg ttgtttggac tcctgcccgc tgatcgagcc caggcgcaaa
     6781 cgaagcttgc cgccaagatg gcgtgtgcca agccggaccc gaattacacc gctcccgttg
     6841 gcgacgaagc caatcacgtc ctgacccttg tctcccagaa atgcacctgg tcgcaggggg
     6901 accttggcgg cgacaagctc aaggacgaga ccgatacgtt cacctcggat gtcaacggca
     6961 atctgtcgca cgatcggggg tacggcgctg ggagcgtggc cagcggcgac aagtacgtcg
     7021 tcaagttcaa gggcacgacc acgctgaaag gcgagaaacc ggtgagcgcc gactgcacct
     7081 ggaccttcaa gagcggcacc ggcaagctga agggtcttac cgggaagggc acctgcaagg
     7141 gcaccttcag cgccgacggt acgtcgacct gggacatcca gggcgagtac aagatttcct
     7201 gaccggcgct gaaggcggca gctgcgctag ccgaacgggt tctcgtcggc ggtcggccca
     7261 tatatagaag gaagggggac ccccagctcc cgaaggtaca ccgtaagctg accccgatgg
     7321 tgataccagt ggttgagcat gatcgaccgc agcatcgcca tccgtggtac cgccagcagc
     7381 tcgcgatcac cactcatcat gcgccacgtg gccagcagcg tggtgtcgtc cattccgccc
     7441 aggaccgtct ttgcttttgc gacgctctgg tcgagcgcgg ggatcagctc agcgctcttt
     7501 gccggagtgg ggtccgggcc gaactccggc gcctgggcgg gcgagggtga cgcgacgaac
     7561 tcagcgaccg cgccgggcac gatcgcgatg tgccaggcga gctggcccag gcttctcgac
     7621 ttgttgtggg gccgccagtc cagccggttg tcggggaccc gctcgagaat tcggcgcgtg
     7681 ctccgcgcct catcctcgag ctcctgcagc atgccgtcga tgagtgccat ggtcctgccc
     7741 tacttcttct cggcccgacg ccggatccgc tcgaccagaa gcggccagcc ctgtcccagc
     7801 gcctcgcggt agccctcggg aaacaggccg agcgccgagt ggtggaaggt gatggtggtc
     7861 ccgccgttct cttccttgag ccggtactgg acattcgagg ccacgccgaa cgacatcatc
     7921 agggggccgt tgatctcgag cagcgtgggc cgcttgatcg cctgcacgtg gccccagaga
     7981 tgtccgttgt tgtcgcccag gtcgcggaac cagcggccgc caggccaggc ctcgagcttc
     8041 atcggcatgg gggtgtcgtt gtggcccttg ttcgaggggc cgatctcctc gagcagcgcg
     8101 gcgaaggtgg tggcgatggg cgcgttgatg tgcacctctt cggcgatgct gagcgacagg
     8161 ttatcgatgc ctggggcgat cgcggtcatg ttgctctcct ttggtggctt gctcggcgcg
     8221 ttccttgact cggctgagct ggtcgcgcca gtagcgttcg aaacggctgg tccattcgtg
     8281 gacgggcttg atagcctccg cgttcgtacg ataaaacgcg tggcggcctt cgcgccgcac
     8341 ctccaccagg ccaacctcga ggagcacccg gaggtgcttg gagacagacg gctgtgccag
     8401 gcccagggat tcgacgatgt cgccgacggg gcgttcggcg ggggccagga agtcgaggat
     8461 gtcccggcgg cggggttcgg ccacggcgtt gaagctgtcg gcggtggtgg cggcgcgagg
     8521 cataggcgaa ttgtattccg atacaggaat atgtcaaggt gagcggtttc tctggcaaca
     8581 cacaggtttg acagcgcagg aattcaagac ccccgaagcc tatcgtccta ttctccttgc
     8641 cccggagaat caagcggccg tacaagcgcg agccatcgta cgcccgagaa ggtgctgcca
     8701 ctggggagaa cacatggatc tcggcctgag tggtcaggtc gccgttgtga ctggtgccag
     8761 caaaggcatc ggactggcgg tgacgaaggc gctggtgggt gaaggggtgc gggtggtcgc
     8821 cggcgcccga aacgtgggag gcgagctcgg cgcgctcgca cagggcggag ccgtccgtgc
     8881 actggagctg gatctctcga ccagagacgg accgcagaag ctggtggatg gcgctgccga
     8941 ctatggtcgg ctcgacatcc tggtcaacaa tgtcggcgcg gtgaaactgc gactggaagg
     9001 tttactgagc gtgagcgacg atgactggtt ggcctcgctg aacctgaatc tcatgtcggc
     9061 ggttcgagcc actcgcgccg cgctgccggt gatgctcaag agtggcaggg gcagcatcgt
     9121 cacggtcagc tcggtgaact ccttcctt
//
LOCUS       DASRJQ010000425         2893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1236833, whole genome shotgun sequence.
ACCESSION   DASRJQ010000425 DASRJQ010000000
VERSION     DASRJQ010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236833"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /gene="modB"
                     /locus_tag="VFB89_09635"
     CDS             <1..630
                     /gene="modB"
                     /locus_tag="VFB89_09635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HZM27607.1"
                     /translation="AALATVLGLPVAYLLATREFRGKHVLEAIIALPMVLPPTVAGVG
                     LLLAFGRAGLAGRALGVFGLSIPFTTTAVVLAEAFIAAPFFIGAAAAGFREVESRYMD
                     AAATLRAAPVTAFVRVLLPLAAPSVLAGATMCWARAIGEFGATITFAGNLPGVTQTMP
                     LAVYVALQTDLDAAIALSVLLVLVSLTILVAVRAFPFGPAALRGQRRAG"
     gene            590..1681
                     /locus_tag="VFB89_09640"
     CDS             590..1681
                     /locus_tag="VFB89_09640"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZM27608.1"
                     /translation="MGLPRFVVSAGLVKRLGGLSLAATVEIEAGSTLALVGESGAGKT
                     TVLRLLAGLTAPDDGHIAVDNQTWFDHKGGVSLPTWQREIGYLSQDYALFPHLSVFEN
                     IAFGLRAQHVDGPQIRQQVEAMLFRFDLEELKTRKPHQLSGGQQQRVALARALVLQPR
                     LLLLDEPLSALDLKTRRSVRAQLRQTLASLPGATVYVTHNPMEAIVFGDRISVLESGR
                     VTQNGLPDDLLRHPRSAYVAEFMGVNLLRGSIAAAEGGGLVRVQTPFGAVIAADSGDV
                     GEVLVALSPREITLHLTPPAGSAQNVFFGPIVELVPEPPFGDRIRVALATSPPLVAEV
                     TRHAVETLRLREGLEVHAAFKATGLVPYR"
     gene            1756..>2893
                     /locus_tag="VFB89_09645"
     CDS             1756..>2893
                     /locus_tag="VFB89_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZM27609.1"
                     /translation="MSSPFKFPVPHNEPVLAYDPGSPAKASLKAALKSIAAQKEEIPI
                     VIGGREIRTGRTEPVTMPHCHARVLADVHQATPELVEQAVDAALEAQREWAQWRFEDR
                     AAVFLKAADLLATRYRPLVNGATMLGQSKTAHQAEIDSACELIDFLRFNVHFAERLYR
                     EQPESARGTWNRMDHRPLEGFVYAITPFNFTAIGGNLPTAPALLGNVVVWKPAGTAML
                     SNWHFFRILEEAGLPPGVINFLPGNSVEISDTLLAHRMLAGIHFTGSTEVFRLLQQRV
                     AHTLEKYVSYPRIVGETGGKDFVLAHASADVNALAVALLRGAYEYQGQKCSAASRAYV
                     PDNLWPALRDRLVGMIGEIRMGDVSDFRNFMGAVIDRRAFERITG"
BASE COUNT          431 a          934 c          940 g          588 t
ORIGIN      
        1 gcggcgttgg cgacggtcct cggcctgccg gtcgcgtacc tgctcgccac tcgggagttc
       61 cgcggcaagc acgtcctcga ggccatcatt gcgctgccca tggtgttgcc accgacggtc
      121 gccggggtgg gcttgctgtt ggcattcggg cgggccgggc tggccggtcg tgcactcggg
      181 gttttcggat tgagcatccc cttcactacc actgccgtgg tgctcgccga ggcgttcatc
      241 gctgcgccgt tcttcatcgg ggcggccgct gccggattcc gggaagtgga gtctcggtac
      301 atggacgctg cggcgacgct ccgggctgca ccggtcacgg cgttcgttcg cgtcctcctg
      361 ccgctcgccg ctccttcggt actggcggga gccaccatgt gctgggcgcg agccatcggt
      421 gagttcggcg cgacgataac attcgccggc aaccttcccg gcgtgaccca gacgatgcca
      481 cttgccgtct acgtggcgtt gcagaccgat ctggatgcgg cgatcgctct ttctgtgctt
      541 ctggtgttgg tctcgctcac cattctcgtg gctgtccggg cctttccgtt tgggcctgcc
      601 gcgcttcgtg gtcagcgccg ggctggttaa gcgtctcggt ggtttgtcgc tggcggccac
      661 ggtcgaaatc gaggccggca gcacgctcgc tctggtggga gagagcggag cgggcaagac
      721 caccgtgctc cgtctcctcg ccggcctgac cgctccggat gacggccata tcgcggtcga
      781 caatcaaacc tggttcgatc acaaaggcgg agtctcgctc cccacgtggc agcgggagat
      841 tgggtatttg tcgcaggact acgctctctt cccgcacctc tccgtattcg agaacatcgc
      901 attcggcctc cgggcgcaac atgtagatgg cccccagatc cgccagcagg tggaggcgat
      961 gctgtttcgc ttcgacctcg aggagctgaa gacacggaag ccgcatcaac tctcgggagg
     1021 ccagcagcag cgtgtcgcgc tggcgcgcgc ccttgtgctg cagccccgtt tgctgttgct
     1081 ggacgagccg ctgtcggctc tcgatctcaa gacccggcgt tcggttcggg ctcagctgcg
     1141 tcagactctc gcctctcttc ccggcgctac cgtctatgtg acccacaatc caatggaagc
     1201 gatcgtcttc ggcgatcgga tctccgtgct cgaatcgggc cgggtcactc agaacggcct
     1261 gccggatgat ctgctgcgac atccacgctc cgcctatgtc gccgagttca tgggggtcaa
     1321 tcttctgcgg ggatcgattg cggcggcgga aggcggcggc ctggttcgcg tccagacgcc
     1381 tttcggcgcg gtgatcgccg cggactccgg agatgtgggt gaagttctgg tcgcgctcag
     1441 tccacgcgag attacgctcc atctcacgcc accggcagga agcgcgcaga atgttttttt
     1501 cggccctatc gtcgagctgg tccccgagcc gcccttcggc gaccggattc gggttgcgct
     1561 cgctacctcg cctcctctgg tcgccgaggt gactcggcat gccgtcgaga cgttgcggtt
     1621 gcgggagggg ctcgaggtcc acgcggcgtt caaggccacg gggctcgtgc cctatcggta
     1681 gcgccaattt gaaaccgccc gtttgaggcg gggccgggtt accttttctt cctctttagg
     1741 cccaggttcg cccgcatgag cagcccgttc aagttcccgg ttccgcacaa tgagccggtt
     1801 ctcgcttacg atcccggaag ccccgccaag gcatcactaa aggcagctct caaatcgatc
     1861 gccgcgcaga aggaagagat tccgattgtg attggcggcc gggagattcg caccggtcgc
     1921 accgagccgg tcacgatgcc ccattgccat gcccgcgttc tggccgacgt gcaccaggcc
     1981 acgccggagc tggtggagca ggcggtcgat gccgcgctcg aggcccagcg ggagtgggct
     2041 cagtggcgct tcgaggatcg ggccgccgtg ttcctgaagg cggccgacct gctcgccacc
     2101 cggtatcgtc cgctcgtcaa cggagcgacc atgctgggcc agagcaagac cgctcatcag
     2161 gccgaaatcg acagtgcctg cgagctgatc gatttcctcc gattcaacgt tcatttcgcg
     2221 gaacggttgt atcgggagca gcccgagtcg gcgcggggta cctggaaccg gatggatcat
     2281 cgtccgctcg agggattcgt ctacgcgatt acgccgttca acttcacggc gatcggcggt
     2341 aatctgccca ctgctcccgc acttcttggc aacgtggtcg tctggaagcc tgcgggaacc
     2401 gcgatgctca gcaactggca cttcttccgg atcctggaag aggccggtct gccgcccggc
     2461 gttatcaact tcttgcccgg caactcggtc gaaatcagtg acaccctgct ggcgcaccgc
     2521 atgctggcgg gcatccattt caccgggtcg accgaggtat ttcgcctgct gcagcagcgg
     2581 gtggcgcaca cgctcgagaa gtacgtgagc tatccgcgga tcgttggtga gaccggcggc
     2641 aaggacttcg tgctggccca cgccagcgct gacgtgaatg cgctcgccgt cgcgctgctg
     2701 cgcggcgcgt acgaatatca ggggcagaag tgcagcgctg cctcccgtgc ctatgtcccg
     2761 gacaatctct ggcctgcctt gcgagaccgg ctggttggca tgatcggaga gatccgtatg
     2821 ggagacgtga gcgacttccg caacttcatg ggcgcggtga tcgaccgccg cgcattcgag
     2881 cggattaccg gtc
//
LOCUS       DASRJQ010000426         3769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2564910, whole genome shotgun sequence.
ACCESSION   DASRJQ010000426 DASRJQ010000000
VERSION     DASRJQ010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2564910"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..338)
                     /gene="rpmA"
                     /locus_tag="VFB89_09650"
     CDS             complement(66..338)
                     /gene="rpmA"
                     /locus_tag="VFB89_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HZM27610.1"
                     /translation="MAHKKGVGSSRNGRNSNPQYLGVKRYGGETVTAGSIIVRQRGTR
                     FHPGKNVRRANDDTLFALIDGVVRFEYKDKNRQRVSIYPAPVASTS"
     gene            complement(346..660)
                     /gene="rplU"
                     /locus_tag="VFB89_09655"
     CDS             complement(346..660)
                     /gene="rplU"
                     /locus_tag="VFB89_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HZM27611.1"
                     /translation="MFAIFRTAGKQFRAEKGKTLRLPRLTAEAGAKLTFDEVLLSSDG
                     ETIRAGTPLVKGASVTAEVVGEGKEPKIYVFKFKRRKNYRRKTGHRQHFTEVRITDLK
                     LG"
     gene            complement(762..2432)
                     /locus_tag="VFB89_09660"
     CDS             complement(762..2432)
                     /locus_tag="VFB89_09660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HZM27612.1"
                     /translation="MKREILINGGQRETRVAIMEDDRLVELLVDRPDHRRTVGDIYLG
                     RVEAVLPGIQAAFVDIGQEKSAFLHASDLLEPEEDEDPAEEEEAAEVETAEADPPAEN
                     EAAADERRGWRGRDRRRRNRDKGNGEKAAGDNERRGRRPLPNISEILKKGQNLPVQVT
                     KEPISTKGCRVTAQISLPGRFLVYMPYASKVGVSRKIESKEQRAKLREMVGKLLPKDS
                     GGVIVRTVAEGVTEEHFRREIDSLLARWRRINRKKTFVRRPPALLERETSLTRGLIRD
                     VFSSKVDALWVDTRELYNEIEQYLSQVDPELMKRVHLYSEPIPLFDKFDIESEIRDLF
                     KARCELPTGGSLVIQPTEALVSIDVNTGRYIGKKDPEKTILRTNLEAAREIARQIRLR
                     DIGGIIVCDFIDMESRANRDKVLQELRTHLGRDRARTKALAVSELGLVEMTRQRVRAS
                     LWHSMTTECPTCGGSGRVFTPEVVARRLERAIKRVGHERREQQISVRLHPEVALYLLE
                     EEPKLLQQLQKATGIDLDLRDDPMMRLDEFRLMSRPAGRDVTELYAVA"
     gene            complement(2907..>3769)
                     /locus_tag="VFB89_09665"
     CDS             complement(2907..>3769)
                     /locus_tag="VFB89_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZM27613.1"
                     /translation="QVLGGFGLTEAGAGSDAGGTATTAVDRGDHFLLNGAKVFITHAG
                     VGEIFSVTARTQPGHGNRGITSFIVTKDTVDLERCRAIGVGHCEDLPKIKGVRAGKKE
                     EKMGWRASDTRELVFEDAIVPKENVLGEPGQGFTNFLKTLDAGRIGIAALSLGIAEGA
                     FEQALSYAGIRKQFGKPIGSFQGVSFQLADMAMEIEAGTHLLYHAAWLKQNGKPFKKE
                     AAMAKLFCSELAMRATTKAVQIFGGYGYTTDYPVERMMRDAKVCEIGEGTSEIQRIVI
                     ARQILGDIAH"
BASE COUNT          648 a         1231 c         1137 g          753 t
ORIGIN      
        1 caaataaaac acgaatgggg tgcggaactc gttccgcacc ccactctccg ccctgccggc
       61 tgccgctagc tggttgaggc aaccggggcg ggatagatcg acacccgctg ccggttcttg
      121 tccttgtact cgaagcggac cacgccgtcg atcaacgcga acagcgtatc atcgttggcg
      181 cgccggacat tcttccccgg atgaaacctc gtaccccgct gccggacgat gatgctgccg
      241 gcggtgacag tctctcctcc gtatcgcttc acaccaaggt attgcgggtt gctgttccgg
      301 ccgttccggc tcgagccgac gcccttctta tgtgccatgt gcgcctcagc cgagcttgag
      361 gtccgtgatt cggacctcgg taaaatgctg ccgatgtccg gtcttgcggc gatagttctt
      421 ccgccgcttg aacttgaaga cgtagatctt gggctctttg ccctcgccaa ccacctcggc
      481 cgtgaccgaa gcgcctttga ccagcggagt tccggcgcgg atcgtctcgc cgtccgatga
      541 gagcagcacc tcgtcgaacg tgagcttggc acccgcttcc gccgtgaggc gagggagccg
      601 gagcgtcttt cccttttcgg cgcggaactg cttgcccgcg gtccggaaga tggcgaacat
      661 ggcgttgtgt cccgagtcgt gatcgtcgaa aacagcgtgc aaacctagcc aggcgttggg
      721 ggttcggaca aggggaactg ggatagatcg ctggactgtg gctacgccac ggcgtatagc
      781 tcggttacgt cccggccagc cggccgactc atgaggcgga actcgtccag gcgcatcatc
      841 ggatcgtccc gcagatcgag atcgatgccg gtggccttct ggagctgctg cagcagcttc
      901 ggttcttcct cgagcaggta gagcgcgacc tccggatgta accgcaccga gatctgctgc
      961 tcgcggcgct cgtggcctac tcgcttgatg gcgcgctcga ggcgccgagc caccacctca
     1021 ggcgtaaaca cgcgaccgga tccgccacag gtgggacatt cggtggtcat ggaatgccaa
     1081 agcgatgccc gtacccgctg ccgggtcatc tcgaccagcc cgagctccga tacggccagc
     1141 gccttggtgc gcgcccgatc gcggcccagg tgagtgcgca gctcctggag caccttgtcg
     1201 cggttggctc gcgactccat atcgatgaag tcgcagacga tgatgcctcc gatgtcgcgc
     1261 agccgaatct gccgcgcgat ctcgcgcgcg gcctcgagat tggtcctgag aatggtcttc
     1321 tcggggtctt tcttgccgat gtagcggccc gtgttcacgt cgatggaaac caatgcttcg
     1381 gtcggctgaa tgacgaggga tccacccgtc ggcagctcac accgcgcttt gaagaggtcg
     1441 cgaatctcgg attcgatgtc gaacttgtcg aagagcggga tcggctcgga atacaggtgg
     1501 acccgcttca tcagctcggg atcgacctgg ctcaggtact gctcgatctc attgtatagc
     1561 tcacgagtgt ccacccagag tgcatccacc ttggagctga acacgtcgcg gatgagcccc
     1621 cgggtgagac tggtttcccg ctcgagcaga gccggcggtc ggcgaacaaa cgtttttttg
     1681 cggttgatgc ggcgccagcg cgcaagcagc gaatcaatct cacgccggaa gtgctcctca
     1741 gtcacaccct cggcgaccgt ccggacgatc actccgccac tgtccttggg cagcagcttg
     1801 cccaccattt ccctcagctt ggcccgctgc tccttgctct cgatcttgcg actgacgccg
     1861 accttggacg cgtacggcat gtagaccagg aagcgaccag gcagggagat ttgcgcagtg
     1921 acccgacatc ctttggtgct tattggctcc ttggtcacct ggactggaag gttctgaccc
     1981 ttcttgagaa tctccgagat gttgggtagg ggacgccggc ctcggcgttc gttgtctccc
     2041 gccgccttct ctccgtttcc tttgtcccgg tttctccgcc tccgatcgcg tccgcgccat
     2101 ccgcggcgct cgtcggcggc tgcttcgttc tcagccggcg gatcggcctc ggccgtttca
     2161 acctccgctg cctcttcttc ctctgccggg tcttcgtctt cctctggctc gagcagatcg
     2221 gaggcatgga ggaaggcgct cttctcctgc ccgatatcca cgaacgccgc ctggataccg
     2281 ggaaggaccg cttcaactcg gccgaggtag atgtcgccga cggtacgacg gtggtcgggg
     2341 cgatcaacca ataactcgac cagacggtcg tcttccatga tggcgacccg cgtttcccgc
     2401 tggccaccgt tgatcagaat ttcgcgcttc aagtccgtaa cctggcaggc cgcgcaaggc
     2461 ggtcagaccg cgacaggcgc aggcattgtt gttggaggcg ctggccggca ggccgaggtc
     2521 acaatgagac cgagacggcc cagcagaccg gaggcgccgc ggcacaactg aacggcctaa
     2581 atatgtggga ggtgggaggt tagggcaatg ccgcagcggg ggcagtcaag cccggcctga
     2641 acaaccggag ccggagtccg agccacactg tcccaggcag acagacggag atcggcaacg
     2701 ctccgcttag cgggtcgagc catgttgacg gcccaaagcc gcccttcacg ctgaccagcg
     2761 aaagcagctc ggcggaaccg aagtagagcc caaacgcgag cgcgcgcact ccgagcgtcc
     2821 ctccctcggc ccccaaccca ccgttggcat cgacgtgaac ctggatcgag aacgctggaa
     2881 agcgtcaggc gctcgcggga tggagcctag tgcgcgatgt cacccagaat ctgacgtgcg
     2941 atcacgatgc gctggatctc gctggttccc tctccgatct cgcacacctt cgcgtcgcgc
     3001 atcattcgct cgaccggata gtctgtggtg tagccatatc cgccgaaaat ctggaccgct
     3061 ttggtcgtcg cccgcattgc cagctcggag cagaagagct tggccattgc cgcttccttc
     3121 ttgaacggct tgccgttctg cttgagccac gccgcgtggt acaggagatg agttcccgct
     3181 tcaatttcca tggccatgtc ggcgagctga aagctcacgc cctgaaagct gccgatcggc
     3241 ttgccgaact gcttgcggat accggcatac gaaagggcct gctcaaaggc cccttccgcg
     3301 atccccaatg acaacgccgc gatgccgatg cgtccggcat cgagggtctt gagaaagttg
     3361 gtgaatccct ggccgggctc gcccagcaca ttctccttgg ggacgatcgc atcttcgaac
     3421 acgagctcgc gagtatcgct cgcgcgccat cccatcttct cttctttctt cccggcgcgt
     3481 acccccttga tcttgggaag gtcctcgcaa tgccccacac cgatggcccg gcaccgttcg
     3541 agatccaccg tgtccttggt aacgatgaag ctggtgatgc cgcgattgcc gtggcccggc
     3601 tgggtccggg cggtcacgga aaagatctcg cctacacccg catgggtgat gaacaccttc
     3661 gcgccgttga gcaggaagtg gtcgcctctg tccaccgccg tggttgcggt gccgcccgcg
     3721 tcgctccctg cgcccgcttc ggtcaacccg aacccgccca gtacctgtc
//
LOCUS       DASRJQ010000427         2792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2565732, whole genome shotgun sequence.
ACCESSION   DASRJQ010000427 DASRJQ010000000
VERSION     DASRJQ010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2565732"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VFB89_09670"
     CDS             complement(<1..293)
                     /locus_tag="VFB89_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27614.1"
                     /translation="MTNQVNKEQSTTRYSRRAFLRRVGAGAGAVALSGGVGAAIAPRA
                     VAAPSATVRTSPTKFGRIFRRLPAFSRATDAVRDALSDLGKPGGLLDAQDKLS"
     gene            complement(449..1303)
                     /locus_tag="VFB89_09675"
     CDS             complement(449..1303)
                     /locus_tag="VFB89_09675"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HZM27615.1"
                     /translation="MSTTTSTQRPAHTQSLVRRHPLISFYVLTYAVSWLLWAPLVIFG
                     DRVPGPLAFILLLLGSLVPSTMGVLFVALLRGRSGVRTLLGRLLHARIGLRWYLAVVA
                     LTMLAPLAVGVSILMGGDTPVVDNTVFGVLFLFAFMIFPGSALGEELGWRGFVLPRMQ
                     ARHSALKASLLIGILWGPWHLPLWLTGSEGHPISLYGPFVVAVVASSVFYTWLYNNTG
                     GSLLIVVLYHAASNLPITVLITPLGGQMAQPFLIYVALTVVAAAAIVIATGAQHLSRT
                     AHRQIEQP"
     gene            complement(1313..>2792)
                     /locus_tag="VFB89_09680"
     CDS             complement(1313..>2792)
                     /locus_tag="VFB89_09680"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZM27616.1"
                     /translation="GPDLAAIDRYVRSEMDAQRIPGLALGIVHGDRIVHVQGFGQADR
                     SRRNVTPETPFLIGSVTKSFTALAIMQLSEAGRLQLDAPVQRYLPWWRVADPGASARV
                     TVRHLLYQVSGLSKATGNAYATSGDTHDSAIEDRVRALRDAELTQPVGSTWQYSNANY
                     WTLGMIVQAASGQSYETYIQQHVFDPLQMHNSHTSGTEAVQDGLPTGHRYWYGFPVAS
                     EMPFDRGGLSSGGLSASAQDMTRYLSLYLNDGRSGTAALISPAGAAELQRAGVPTGLD
                     GVSYAMGWDVGPFHGIPTISHDGSGFDSHANVVLIPDRRWGVVVMENGENSPDEFFGS
                     RRMTGIAYGLAGLLIGEEPAATSSSVSLGVAYGVVLGILLIQVAGMVRSVRIIRTWRT
                     VPSRRPTGALRVGLRVGLPLLASWTWALIVLVGLPQMIRAPLPAVLMGLPDLGYPLVA
                     SAVLAFGWGLVRAIWAIRILRTPPGVPGSSNGATSQLSPTTR"
BASE COUNT          519 a          880 c          958 g          435 t
ORIGIN      
        1 ttgctcagct tgtcctgggc atcgagcagg ccgcccggct tgcccagatc ggaaagcgca
       61 tcccgcaccg cgtcggttgc gcgggagaac gcaggcagcc tccggaatat gcgtccgaac
      121 ttcgtcggac tggttcgtac cgtcgcgctc ggcgcggcca ccgcgcgggg cgcgatcgcg
      181 gcacccacac cgcctgagag cgcaaccgca cctgctccgg cgcctacgcg cctcaggaag
      241 gctcgcctgc tgtagcgagt ggtggactgt tctttgttga cttggtttgt cactgcggcc
      301 ccgtccgctg tccaagcccc cagcgtgcgt acgttatcaa atcgagatat ttatcaccat
      361 aggtcaccgc gattagtctc aatttggtaa cgagcggtca cgctgaagta gacgcctcag
      421 tcatcggggc accgcaggcc tctgggggtc acggctgctc gatctgcctg tgggcggtgc
      481 gggacagatg ctgggcgccg gtcgcgatca caatggctgc cgccgcgacg acagtgagcg
      541 cgacatagat aaggaagggc tgcgccatct gaccgccgag aggagtgatg agtaccgtga
      601 tcggcaggtt gctcgctgcg tggtagagca cgactatgag caggctgcca cctgtgttgt
      661 tgtacagcca ggtgtagaac acggatgagg cgactaccgc gacgacgaag ggaccgtaga
      721 ggctgatcgg atggccctcg ctcccggtca gccacagcgg caggtgccag ggtccccaga
      781 ggatcccgat cagaaggctg gccttcaggg cactgtggcg ggcctgcata cgtggtagga
      841 cgaaaccccg ccatccgagt tcttcaccca aagcgctgcc cgggaaaatc atgaacgcga
      901 acaggaacag gacgccgaag acggtgttgt ccacgaccgg ggtgtcccca cccatcagga
      961 tgctcacgcc gaccgcgagt ggggccaaca tggtcagggc caccaccgcc aagtaccagc
     1021 gcaacccgat ccgggcatgt aggaggcgcc cgagcagtgt ccgcacgccg gatctgccgc
     1081 ggaggagggc gacgaacagt acgcccattg tcgacggcac gagggatccc agcaggagaa
     1141 ggatgaatgc cagcgggccg gggacgcgat cgccgaagat gaccaggggg gcccacagca
     1201 accacgagac ggcgtaggtc aggacataga agctgatcaa ggggtgacgc cgaaccaggc
     1261 tctgtgtgtg agctgggcgc tgggtggaag tggtggtgga catcgattct ccttaacgag
     1321 ttgtagggct cagctgggag gtagcgccgt tcgaggagcc aggcacaccg ggaggcgtgc
     1381 gaagaatccg gattgcccag atcgctctga ccagtcccca accgaaggcg aggacggcgc
     1441 tggcaaccag cggatagccg aggtcgggca gccccatcag taccgccggc agcggagcac
     1501 gaatcatctg tggcagtcca acaagcacga tgagggccca ggtccagctg gccaggaggg
     1561 gcaggcctac gcggagcccg actcgcagcg cccccgtcgg acgtcgcgat gggactgtgc
     1621 gccacgtacg gataatccgc acgctccgga ccatcccggc gacctgaatg agaaggatcc
     1681 ccaggacgac tccgtaggcc acccccagcg acacgctcga cgacgtcgcg gctggctcct
     1741 cgccgatcaa caggccggcg agaccgtaag cgatgcccgt catgcggcgc gagccgaaga
     1801 actcgtctgg ggagttctcc ccgttctcca tcaccacgac gccccaccgg cggtcgggga
     1861 tgaggacgac gtttgcgtgg gagtcgaagc cactgccgtc gtgggagatc gtcgggatgc
     1921 cgtggaacgg gccgacgtcc cagcccatcg cgtaggagac gccatccagg ccggtgggaa
     1981 ctcctgcccg ctgcagctcg gcggcaccgg caggtgagat cagcgccgcg gtgccagatc
     2041 ggccatcgtt gaggtagagg ctgaggtagc gggtcatgtc ctgtgcgctg gcgctgaggc
     2101 cgccggaact caggccgcct cggtcgaagg gcatctcaga ggcgacgggg aagccgtacc
     2161 agtagcggtg gccagtcggg aggccgtcct gcaccgcttc ggtccccgac gtgtgggagt
     2221 tgtgcatctg cagcgggtcg aaaacgtgct gctggatgta agtctcgtaa gactggccgg
     2281 aggcggcctg cacgatcatg ccgagcgtcc agtagttggc gttgctgtac tgccaggtcg
     2341 aaccaaccgg ctgggtgagc tcagcatcgc gcagcgcccg aacccggtcc tcgatggcgg
     2401 agtcgtgagt gtccccgctg gtcgcgtacg cgttgcccgt ggccttcgac agcccgctga
     2461 cctggtacag cagatggcgg acggtgaccc gggccgacgc gcccggatcg gcaacccgcc
     2521 accacggtag gtagcgttgc accggcgcgt ccagttgcag ccggcccgcc tcgctcagct
     2581 gcatgatcgc cagtgcggtg aaggacttgg tcactgaacc gatgaggaac ggcgtttccg
     2641 gggtcacgtt ccgtcgggac ctgtccgcct gtccgaagcc ctgcacgtgc acgatgcggt
     2701 ccccgtgcac gatgccgagc gccaatcccg gtatgcgctg agcgtccatc tccgagcgca
     2761 cgtatcggtc gatggcagcc aggtccgggc cc
//
LOCUS       DASRJQ010000428         3168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2767426, whole genome shotgun sequence.
ACCESSION   DASRJQ010000428 DASRJQ010000000
VERSION     DASRJQ010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2767426"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VFB89_09685"
     CDS             complement(<1..316)
                     /locus_tag="VFB89_09685"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZM27617.1"
                     /translation="MATEVGLVVEDDSKSRKLVRDLLTVKGYEIIEAETGEEGVALAQ
                     ERRPSLILMDIRLPGIDGIQALERLRAEAATQGIPVMAMTASVMSGDRQRVLDAGFDA
                     FQS"
     gene            complement(306..>3168)
                     /locus_tag="VFB89_09690"
     CDS             complement(306..>3168)
                     /locus_tag="VFB89_09690"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZM27618.1"
                     /translation="VTEALEQQTATSDVLRVISQAQTDLQPVFDALIDNATRLCGADM
                     GALTRFDGEVLRFVLHSGMPAEVQAFWQQNPVRPGRDSVTGRAALERRTIHVPDVLTE
                     PAYHSGFLREGQAVTGYHSALVAPMLRDGALIGTIAMLRREVRPFTASQIKLLETFAD
                     QAVIAIENVRLFTELQARNSDLTATSEILQVISRSPTSAQPVFEAIAESAARLTDAVF
                     GGTFLVADEMLHLAAIHIPAGGPNDAFMRSYPVPLDENTLGTRVARERIVVNISDTDT
                     EPSLPESQRERLRMLGARSLLIVPMVRGAQAVGMIIAARRQAIAFTDKQVALLQTFAD
                     QAVIAIENVRLFNELQTSNQELTTSLDKQTATSDILRVISQSPTDVQPVFDAIVQSAV
                     RLCGAIYGNVYKVNGGEIHLVAHHNLMPEQLAQWQQQYPRPVSGSGAVPRAIRTGTVG
                     LTADWEADSEWGVPSAGVQAAQRARGVRSILIVPMFRQQEVIGAIGLTHREVAAFTNA
                     HVELLQTFADQAVIAIENARLFKELQARTQDLTRSVGELRALGEVGQAISSTLDLETV
                     LNTIVSRAVELSGLDGGVVFEYDDASEEFVQRVATETGGALAEARRTTRVRKGEGVLG
                     RTAITLEPVQVPDITAPGAYQSRLRENLIASGIRAILAVPMVREDRLIGCLAVTRTQP
                     GEFPAETIELLRTFATQSALAIQNARLFLEIEDKSRQLEVASQHKSEFLANMSHELRT
                     PLNAIIGFSEVLTDRMFGELNEKQDEYLKDIYASGQHLLSLINDILDLSKIEAGRMEL
                     ELADFHLPQAIDNALVLVRERALRRGIKLEPSIDSHLGEIRGDERKIKQVLLNLLSNA
                     IKFTPEGGRVGVRAEPVDEHVKVAVSDTGVGIAPEDHEAVFEEFRQVGTAEKKAEGTG
                     LGLALARKFIELHGGRIWVQSQVGVGSTFTFTLPVRHGD"
BASE COUNT          552 a         1049 c          970 g          597 t
ORIGIN      
        1 tgctctggaa ggcgtcgaat cctgcgtcca acacccgctg ccgatccccg gacatcaccg
       61 acgcggtcat ggccatgacg ggaatcccct gagtggcagc ctcggctcgc agccgttcga
      121 gcgcctggat cccgtcgatg ccgggtagtc gaatgtccat caagatcagg ctcgggcggc
      181 gctcttgggc gagtgccaca ccctcttctc ccgtctcggc ctctatgatc tcgtagccct
      241 tgaccgtcag aagatcccga acgagctttc gactcttgct gtcgtcctcg acgacaaggc
      301 cgacctcagt cgccatggcg caccggaagc gtgaacgtga aggtcgaacc gacaccaacc
      361 tggctctgga cccagatccg gcccccgtgc agctcgatga acttccgcgc cagggcaagt
      421 ccgagacctg ttccctccgc cttcttctct gccgtcccca cctgccgaaa ctcttcaaac
      481 accgcctcat gatcctccgg cgcgataccc acgccggtat cgctgaccgc caccttcacg
      541 tgctcgtcca cgggttccgc tcgaactccg acccgcccgc cttccggcgt gaacttgata
      601 gcattggaca gcaggttcaa gagcacctgc ttgatcttcc gctcgtcgcc ccgaatctcg
      661 ccgaggtgcg aatcgatcga tggctccagc ttgatgccgc ggcgcaacgc ccgctcccgg
      721 acgaggacca gggcattgtc gatggcttgg ggcaagtgga agtcggccaa ctccagctcc
      781 atgcggccag cctcgatctt cgacaagtcg aggatgtcat tgatcaggga caggaggtgt
      841 tgcccagaag cgtaaatgtc cttcaggtac tcgtcctgct tctcgttcaa ctcgccgaac
      901 atgcggtcgg tcagcacctc ggagaagccg atgatggcgt tcagcggtgt ccgcagctca
      961 tgggacatgt tcgccaagaa ttcggacttg tgctggctgg cgacttcgag ctggcggctc
     1021 ttgtcttcga tctctaggaa caggcgtgcg ttctgaatgg ccagggcgga ctgggtggcg
     1081 aaggtccgca gaagctcgat cgtttcggcc gggaactctc cgggttgggt gcgggtcacg
     1141 gcgagacagc cgataagccg atcttcgcgg accatcggta cggccaagat ggcccggata
     1201 ccggacgcaa tgaggttctc ccgcaatcgg ctctggtagg cgcccggggc ggtaatgtcg
     1261 ggcacctgaa ccggttccag cgtgatggcg gtccgcccga gcactccttc ccctttccga
     1321 accctcgtgg tacggcgagc ttcggccagg gctccaccgg tctcggtcgc cacccgctgg
     1381 acaaactcct cagaggcgtc gtcgtactcg aagaccacgc caccgtcgag ccccgacagc
     1441 tcaacagcac gcgacacgat cgtgttcagc accgtctcga gatcgagagt ggaactgatc
     1501 gcctgaccga cctcgccgag cgctcgaagc tcgcccaccg accgcgttag gtcctgggtc
     1561 cgcgcctgca gttccttgaa cagcctagcg ttctcgatgg cgatcactgc ttggtccgcg
     1621 aaggtctgca gcagctccac gtgggcgttc gtgaatgctg cgacctcgcg gtgagtcagg
     1681 ccaatggcgc cgatgacctc ctgctgtcgg aacatgggga cgatgagtat gctccgcacg
     1741 ccacgcgccc gctgagccgc ctgcacgccc gcgctaggaa caccccattc gctgtcggct
     1801 tcccagtcag ccgtcagacc cacagtgccc gtacggatgg ccctgggaac agccccgctg
     1861 ccgctcactg gacgcgggta ctgctgctgc cactgcgcga gctgttccgg catcaagttg
     1921 tggtgagcca ctaagtggat ctcgccgccg ttcaccttgt aaacgtttcc atagatcgcg
     1981 ccgcagagcc ggacggcact ctgcactatc gcgtcgaaaa ccggctgaac gtcagtcggc
     2041 gactggctga tgacacggag gatgtcgctg gtggcggtct gcttgtcgag cgacgtggtc
     2101 aattcctggt tactcgtttg gagctcattg aacagccgca cgttctcgat ggcgatgacg
     2161 gcctggtcgg cgaacgtctg gagcagggcg acctgtttgt cggtgaacgc gatcgcctga
     2221 cgtcgggccg cgatgatcat cccgaccgcc tgtgcgcccc gaaccatcgg cacgatcagc
     2281 aggctgcggg cgccgagcat ccgtaagcgt tcgcgctgag actctggcag cgacggctcg
     2341 gtgtccgtat ccgagatgtt cacgacgatt ctctcgcgcg ccacacgcgt ccccaacgtg
     2401 ttctcgtcga gagggaccgg ataggagcgc atgaaggcgt cgttgggccc accagctggg
     2461 atgtggattg cggcgaggtg caacatttcg tcagcgacga ggaacgtgcc gccgaagacc
     2521 gcgtccgtca gccgcgcggc gctctcagca attgcctcga acaccggttg cgcgctcgtc
     2581 ggcgagcgcg aaatcacctg caggatctcg ctcgtcgccg taaggtcact gttgcgcgcc
     2641 tgtagctcgg tgaacagccg gacattctca atagcgatga ccgcctggtc ggcgaaggtc
     2701 tcgagcagct tgatctggct ggctgtgaac ggccgaacct cacgtctcag catcgcgatc
     2761 gtgccgatca gggcgccatc ccggagcatg ggcgccacga gcgcagagtg gtacccggtt
     2821 actgcctggc cctcccgcag aaagccagag tgatatgccg gctcagtcag gacgtccggg
     2881 acgtggatcg tccttcgctc gagggcagct ctacccgtga ccgagtcccg cccggggcga
     2941 acggggttct gctgccagaa ggcctggacc tcggccggca tgccgctgtg gagcacaaag
     3001 cgcaagacct cgccgtcgaa gcgagtcaac gcgcccatgt ccgcgccgca gagcctcgtc
     3061 gcgttgtcga tcagcgcatc gaacaccggt tgcaagtcgg tttgcgcctg actgatgacg
     3121 cgcaagacgt cgcttgtggc cgtttgctgc tccaacgcct cggtgacc
//
LOCUS       DASRJQ010000429         4218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_510271, whole genome shotgun sequence.
ACCESSION   DASRJQ010000429 DASRJQ010000000
VERSION     DASRJQ010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510271"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VFB89_09695"
     CDS             <1..316
                     /locus_tag="VFB89_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27619.1"
                     /translation="IQSQIDRDGAILAANDTDITTLGRDTYSYAWPRDGALVASGLIH
                     AGQAGGAERFFGFCSRNMSPNGYMRHKYNPDGSLASSWHSYVRDGKPVLAIQEDETAL
                     VI"
     gene            415..876
                     /locus_tag="VFB89_09700"
     CDS             415..876
                     /locus_tag="VFB89_09700"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HZM27620.1"
                     /translation="MDSSLFGLVLLGALPPDDPQAVSTLQQVEERLWARTDVGGLARY
                     HDDHYQQMERHDLSRVPGNPWFVCTMWLAFYRLQRAQTAEDLVPGLKLLEWASERALP
                     SGTMAEQLHPYTGEPLSVSPLIWSHADASRAGVHPAPLPSKPLPVLCAAGT"
     gene            1392..2390
                     /locus_tag="VFB89_09705"
     CDS             1392..2390
                     /locus_tag="VFB89_09705"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZM27621.1"
                     /translation="MKDGRIPTVHLAVHGATYSHLYWRWPYQPEIYSYADHLSAAGYA
                     VFAVDRIGVGRSSHPPGASVTMNVNAFVIHQLVGALRRGSIGKVAFQRVVLVGHSLGS
                     IAALAESSRYQDVDGLVLTGISHFSPGDASLLPSLVRANRDPRFAGRKLDDDYLTTSP
                     GSRAANFLYSPSSDPRLLALDEAIKETVTTAELKTSVPVLLSGASFAIRVPVLLIAGQ
                     FDPGICGSAPICASTAASAAAFEASERPFFAPEACLQTIIIPDAGPDLNLEPSAPDMY
                     AAIRAWSGAYIGLRQAARPCSEHVRPGSDGIREAHLQRMAASHVGSVDSGTARHPR"
     gene            2801..3403
                     /locus_tag="VFB89_09710"
     CDS             2801..3403
                     /locus_tag="VFB89_09710"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HZM27622.1"
                     /translation="MSSRSIFTGALFLLMPALAVAQAPAAPSETWPQVTVFANGGGAS
                     PLTNLNELGTADFKTGWTAGGGVGVQLNRYLAVRGVFDYARNKGDDISSAYAGQSFNR
                     YFYGADIQLRYPTKSGFAPYLLGGAGAVTIDNKDDPTFNQFTNFAGKVGAGVEYAFPN
                     NGFGVFAQGASYIYEFDHGGFDKTQTDLLWTAGLSYRFRL"
     gene            3421..>4218
                     /locus_tag="VFB89_09715"
     CDS             3421..>4218
                     /locus_tag="VFB89_09715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZM27623.1"
                     /translation="MPLILSTWRAPLRRGATVLMLIACALALLGCSKQTPPPPPPPEV
                     AVLAVEPRTVEDQYEFIGEVQAFRRVEVRAQVSGVIVERPFREGAEVKANDVLYRIDP
                     RTYEAALRGAQARLAESEARLANAMTNADRLRPLLEYKAVARQDVDNAEAELKQARAL
                     VDDARASVDQAQKELDYTVVRAEISGRAGRALLDLGTRVTALSDVLTTIDQLDPVYVS
                     FRPSAQQQLAWKRDPHTARALEAGGSVRVQAMLADGTALPRTGRISFV"
BASE COUNT          697 a         1418 c         1304 g          799 t
ORIGIN      
        1 tattcagagc cagatcgacc gggatggtgc catcctcgcg gcgaatgata cggacatcac
       61 cactctaggc cgggatacgt attcctacgc gtggcctcgc gatggcgcgc tggtcgcgag
      121 tgggctgatt catgccggcc aggccggcgg agctgagcgc tttttcggct tctgttcccg
      181 caacatgtcg ccgaatggct acatgcgcca caagtacaac ccggatggga gtctcgcctc
      241 gagctggcac agctatgttc gcgacggcaa gcccgttctc gcgatccagg aggatgaaac
      301 cgcgctggtc atctaggccc tggccgagta ctacgaccgc tatcgccggg tcgaagaggt
      361 tgccgagttc tatcgacgac tggggactcg ccccgttatg ccctcgatac gactctggat
      421 tcgagtctct ttggactcgt gcttctcggt gcgctgccgc cggacgaccc ccaggcagtc
      481 tccaccctgc agcaggttga ggaacgcctg tgggcccgaa ctgacgtcgg cgggctggct
      541 cgttaccatg atgaccatta ccagcagatg gaacgtcatg acctctcacg agtgccgggc
      601 aatccctggt tcgtctgcac catgtggctc gccttctacc gccttcagcg agctcagaca
      661 gcggaggacc tggtgcccgg gctcaagctc ctcgagtggg ccagcgaacg agcgttgccg
      721 tccgggacaa tggcggagca gctgcatccc tataccggcg agccgctctc ggtctcgccg
      781 ctcatatgga gccatgccga tgcgagtcgt gcaggagtac atccagcgcc actcccgagt
      841 aaacctttgc ccgtgctgtg cgcagccggt acgtgaggcg gatccgagtt cagacggtac
      901 gctcgctggc gctgatcgta atccctccga ggccgtcgcg ttctagcccc tcgatcccac
      961 atcctagcgt tcgtagttct tcactcgacg tcggttcgag tgcgtaggcg tatgcatcgt
     1021 gcgtacggtt acacacgcac gatggggcag catcggcgct cgatggtgat tcgagcgctc
     1081 agttcaccgg catcgtcctt gcccattagg cctcgtgact cgaggtgcgc gaaggcgata
     1141 tgtcgggcaa acaagttcta ctcctctcgc tcttgggagc cataggtacc tggttcctga
     1201 ccaccattga taaccggttg ctgggacctg tatcgcctcc cgctcccgct gttgcaggga
     1261 gccccgccag ttcccccgcc agcatacgaa atagcgcaca acgacaatgc catgcggcga
     1321 cggttcctgt cgccctggca gcgggtgaga cccgccgtta cacggtcttc ggtgagctgt
     1381 gctttccgct ggtgaaggac gggaggattc cgacggtcca tctggctgtt catggcgcta
     1441 cctacagcca cctgtattgg cgttggccgt atcagcccga gatctactcg tacgccgatc
     1501 acctgtcggc ggccggatac gccgtattcg ccgtcgatcg gatcggcgtc ggccggagct
     1561 cgcacccacc cggcgcctcg gtgacgatga acgtcaacgc cttcgtgatc caccagctgg
     1621 ttggggcgct ccggcgcggc tccattggca aggtcgcgtt tcagcgcgtc gtgttggtgg
     1681 ggcattcgct gggatccatt gctgctcttg ccgagtccag ccgataccag gacgtcgacg
     1741 gcctggtgtt gaccggcatc agtcatttct cccccggcga tgcgtccttg ctcccatcgc
     1801 tggttcgggc caaccgcgat ccgcgtttcg ccggccggaa gctcgatgat gactatctga
     1861 ccacgtcgcc ggggtcgcgc gccgccaact tcctctattc gccttcatcc gatcctcggc
     1921 tcctcgcgct cgacgaagcc atcaaagaga ccgtcacgac cgccgagctc aagaccagtg
     1981 tcccagtcct gctctcgggc gccagcttcg ctatccgagt gcccgtgctg ctgattgccg
     2041 gccagttcga cccgggtatc tgcgggagcg cgccgatctg cgccagcact gcggcgagtg
     2101 cagcggcctt cgaggccagc gagaggccgt tcttcgcccc agaagcatgc ctgcagacca
     2161 tcatcatccc ggatgcgggc cctgatctca atctcgagcc gtcggctccg gacatgtacg
     2221 cggcgatccg ggcctggtcc ggtgcgtaca tcggcctgcg ccaggccgcg cggccgtgca
     2281 gcgaacatgt gcgtccgggt agcgatggga tcagggaggc gcacctccaa aggatggcgg
     2341 catcgcatgt cggctcggtg gactccggca cggctcggca cccgcgatga tgtctgcggg
     2401 acctctttag gcacattcgg gatctggtgc cggtttcccc aatcgcgcgc gtttcggtcg
     2461 ctgatacggc gttccccacg ccgatgactc atcggcggtg cgcgacacca gcccgctcct
     2521 cctcagcgaa cagatctcgc ctcaccgcca ggctgcgtac cggcagacac gctgcatatc
     2581 ggtaatcact cgaagcgtgg gcgttgatcg gcgcccgaaa tcggcttcgc cttctaagcg
     2641 cttgtatccc agcccttccg cagatcatcc ctcaggtcct ctggtgcggt ccccaacttg
     2701 ccttcgctgg cctgcagtga tggccatgca gcccggctcg agcgacctgc ccccgcttgc
     2761 agccggattc gagaacaggg gcactcgcaa gggtgaatca atgtcgagcc gttcgatctt
     2821 cacgggtgcg ctgttcctcc tgatgcccgc actggcggtg gcgcaagcgc ctgccgctcc
     2881 gtccgagacc tggccgcagg tcaccgtctt tgccaacggc ggcggagcct cgcccctcac
     2941 caacctcaac gaactcggca ccgccgactt caagaccggc tggaccgcgg gcggcggcgt
     3001 cggcgtccag ctcaataggt atctcgcagt ccgcggggtg ttcgattacg cccgcaataa
     3061 gggagacgac atctcgagcg catacgccgg gcagagcttc aaccgctact tctatggcgc
     3121 cgacatccag ctgcgctacc cgaccaagag cggcttcgct ccctacctcc tgggtggtgc
     3181 gggggccgtc acaatcgaca acaaggacga tccgacgttc aaccagttca ccaatttcgc
     3241 cggcaaagtc ggcgcgggag tcgagtacgc attcccgaac aatggtttcg gggtcttcgc
     3301 ccaaggcgcg tcgtacatct acgagttcga ccacggcggg ttcgacaaga cccaaaccga
     3361 cctcctctgg actgccggtt tgagctaccg cttcagactg taatccgagg atcccgcgct
     3421 atgcctctca tcctatcgac ttggcgcgcc ccgcttcggc ggggcgccac cgttctgatg
     3481 ctgattgcct gcgccctggc cctcctgggg tgcagcaagc agacgccgcc tccaccaccg
     3541 cctccggaag tcgcggtgct ggcggtcgag ccacggacgg tggaagacca gtacgagttc
     3601 atcggcgaag tgcaggcgtt tcggcgggtc gaggtgcgcg cccaggtgag cggtgtgatc
     3661 gtggagcgtc ctttccgcga gggggccgag gtcaaagcca acgatgtgct ctatcgcatc
     3721 gacccgagga cctacgaggc agcccttcga ggcgctcaag cccgcctcgc cgagtctgag
     3781 gcccgcctcg ccaatgccat gacgaacgcc gatcgcctgc ggccgctgct cgagtacaag
     3841 gcggttgcga gacaggacgt ggacaacgcc gaagcggagc tcaagcaggc gcgagcgttg
     3901 gtcgatgacg cccgcgcgtc ggtggaccag gcccagaagg agctcgacta caccgtggtg
     3961 cgcgccgaaa tctcgggtcg tgccggccgg gccctgctcg acctagggac ccgggtcacg
     4021 gccttgagtg atgtgctgac gaccatcgat cagctcgatc cggtgtacgt cagcttccgc
     4081 ccgtcggcgc agcagcagct cgcctggaag cgcgatccgc acaccgctcg agccctggag
     4141 gcgggcggct cggttcgagt gcaagcgatg ctggccgacg gcacggcgct gccccgcacc
     4201 ggccggatca gcttcgtg
//
LOCUS       DASRJQ010000430         3730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2476536, whole genome shotgun sequence.
ACCESSION   DASRJQ010000430 DASRJQ010000000
VERSION     DASRJQ010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2476536"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="VFB89_09720"
     CDS             <1..886
                     /locus_tag="VFB89_09720"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HZM27624.1"
                     /translation="GEPLYIEQIRRLVYRSAFIQAQYPLSRFSRFEMGLEATSADDAI
                     YQTIIPLVSEITVQKIGLGTHNYVAPRLALVHDNSVQGYIGPWAGRRSRFEVAQAVGG
                     WRFTQVTADYRRYDKIAGPVLFATRALMFGRFGRDAQLIQNFIGDPSFLRGQTSGSYS
                     RNECTSALAAPPPTGSASGCVALDRLIGSRMALGSAEIRFPILTRQLIHTLPSAVPPI
                     EAAIFYDIGVAWNNGNTLKWSLEQGDNPELVRTPLQSYGFSVRTNLFGFMILRLDMAR
                     PLDRAIKSLWTLSLGPTW"
     gene            978..2360
                     /locus_tag="VFB89_09725"
     CDS             978..2360
                     /locus_tag="VFB89_09725"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HZM27625.1"
                     /translation="MLPRSITGAVLVVAACGGTDRPVSRLPDSPRAATPASVLRLPQS
                     GGQAHLYRVPSLDESSWKEADKLPAMKRLVGTDLEQEIVYALDAKNNLVGLDLESRRA
                     RTVLTDIREAAVGPDGAVYAVDTGATVTQIIRRSPVRFRTKLAGASRGLYGTMNGTLV
                     ALPAAERGQAMALTPDQAQGRTTLPQGSAAATYLGDLVAVAADTAVVLYEPTAKQPVR
                     SIEVSGKVRAVAFSPSGHRLYVARGKGDIQVFDRLSSELIESIDLPGPARDLRSDFFG
                     SWLLGRPERGDSVWIVDATTGKLAGTVSTRWGSDLPSVAGTKTLLVRAGPDVKALDLG
                     SKKLTTTGSVAGGAEDLWLPLSWLPPLEQQQLAENTDSTAPAADSGTGVPGIFLQVSS
                     SRNPSWANELAGKLKAAGLPASVLPPKTGEDAYRVVLGPYASRDQAEATGRSLGMPSF
                     VITPQDQAPQ"
     gene            2395..>3730
                     /locus_tag="VFB89_09730"
     CDS             2395..>3730
                     /locus_tag="VFB89_09730"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZM27626.1"
                     /translation="MKLTKLELSGFKSFADTVTLDFEDGVTAIVGPNGCGKSNVSDAV
                     RWVLGEQSARLLRGGKMEDVIFQGSANRRPVNVTEVALFLDNTDGALPIAYQEVVIAR
                     RLSRSGQSDYLLNGTVVRLRDIQDLLRGTGLGSDAGVVIEAEMIKLLLSDRADERRSL
                     FEEAAGIGLYRDRKHSTERRLEETAADLQRVEDLLNEVQSQIRSLARQKGKTERSAKL
                     LEEKFAVQVTLARRHLDRLAERTDGLEARFNQLSELLPTEREGVGRADERREEAARAR
                     TAAQVHRTEIARRRGAVRVEVEKLDGDLALAAERLGTATSRRTKAQEERTQTELRAAL
                     ALTERDAAAADRTAAEAEHANINVEVASRADDEESGRQKLVGQRTAVRQLEEDLQSRA
                     QAVRSLEGERTALEGELASLRDRAAQAAAHREGLRSELEAAQRRRDHAIEQAA"
BASE COUNT          664 a         1221 c         1222 g          623 t
ORIGIN      
        1 gggagagccc ctctatatcg aacagattcg ccgtctggtc taccggtcgg ctttcatcca
       61 ggcgcagtac cccttgagcc ggttcagccg attcgagatg ggcttggagg ccaccagtgc
      121 cgatgacgcc atctaccaga ccatcatccc gctcgtctcc gagatcaccg tccaaaagat
      181 cggactgggc acgcataact acgtggcgcc tcggctcgcg ctggtccacg acaactcggt
      241 tcaaggttat atcggtccct gggcgggccg tcgctcgcgg tttgaagtcg cccaagctgt
      301 cggcggctgg cggtttaccc aggtcaccgc ggactaccgc cgttacgaca agattgccgg
      361 gccggtcctc ttcgcgactc gggcgctcat gtttggacgg ttcgggcgcg atgcgcagct
      421 gatccagaac ttcatcggcg acccaagctt cctccgtggg cagacttcag gatcctacag
      481 ccggaacgag tgcaccagtg cactcgcggc gcctccgccc acaggctcgg caagcggttg
      541 cgtggccctc gaccgtctga tcgggtcccg gatggcgctg ggatcggcgg agattcgatt
      601 cccgattctg acgcgccagc tgatccacac gttgccaagc gcggtgccgc ccatcgaagc
      661 ggcaatcttt tacgacattg gcgtcgcctg gaacaacggc aatacgttga agtggagcct
      721 cgagcagggc gacaacccgg agctggtacg gactccactt cagtcctacg gcttttcggt
      781 ccgaactaat ctctttggct tcatgatcct ccggttggat atggcacgac cgctggatcg
      841 agccatcaag agcctctgga cgctgagcct cggacccacc tggtaagctg agcctcaagc
      901 ggctcaaacg gggacttccg cctggaggtc ccccgtttgc cgtttaccgt tcacccctgt
      961 tcatctagtt ttctcttgtg ctccccagat caatcaccgg tgcggtgctc gtggtcgccg
     1021 cttgcggtgg gaccgaccgt cccgtctccc ggcttcccga cagcccccgc gcggcgactc
     1081 cggccagcgt gcttcgactc ccgcaatcgg gtggtcaagc acatctgtac cgggtgccca
     1141 gcctcgatga gtccagctgg aaagaggccg acaagcttcc cgccatgaaa cggctggtcg
     1201 gcaccgatct cgagcaggag atcgtttatg cgctcgacgc gaagaacaac ttggtaggtc
     1261 tggatctcga atcccggaga gcgcgcacgg ttctgaccga cattcgtgag gctgcggtcg
     1321 gaccggacgg cgccgtgtac gccgtggaca ccggagcgac cgtcacccag atcatccggc
     1381 gcagtccggt gcgctttcgg accaagctgg ccggcgcctc ccgcggcctt tacgggacca
     1441 tgaacggtac tttggtcgcg cttcctgccg ccgagcgcgg gcaggccatg gcgctcacac
     1501 cggatcaggc ccagggtcgc accacgttgc cacagggcag cgctgccgcg acctatctgg
     1561 gcgacctggt cgccgtggca gccgacaccg ccgtcgtcct gtacgagccg accgcgaaac
     1621 agccggtacg gtccattgaa gtcagtggga aggtgcgcgc agtcgcgttc tcgccatcgg
     1681 gccatcggct ctacgttgcc cggggcaagg gggacatcca ggtgttcgat cggttgagca
     1741 gcgagctgat cgaaagtatc gatctgccgg gtccggcccg cgaccttcgg agcgatttct
     1801 tcgggagttg gctgctcgga cgaccggagc ggggcgactc ggtctggatc gtggatgcaa
     1861 cgacgggcaa actggcggga acggtctcga cccgctgggg ttccgatctg ccctcggtgg
     1921 ccgggacaaa gactctgctg gtgcgcgcgg gcccggacgt gaaggccctc gatctgggaa
     1981 gcaagaagct gacaacgacc ggctcagtgg ctggtggcgc ggaagacctg tggcttccgt
     2041 tgtcctggct tccgcccctg gagcagcagc agttggcgga gaataccgac agcaccgcgc
     2101 cggccgccga ctcgggcacc ggtgtgccgg gaatctttct tcaggtgagc agctcccgaa
     2161 atccatcctg ggccaatgag cttgctggaa agttgaaagc cgccggcctg ccggcctcgg
     2221 tgctcccgcc caagaccggc gaagacgcgt accgcgtcgt gcttggcccc tacgccagcc
     2281 gcgaccaggc cgaagcaacc ggcaggagtc ttggcatgcc ttcgttcgtg atcactccgc
     2341 aggaccaggc cccgcaatga ggcctggacg caccccgacc acaggatgcc ctgcatgaag
     2401 ctcaccaagc tcgagctctc cggcttcaag tcgttcgccg acaccgtcac gctggacttc
     2461 gaggacgggg tcaccgccat cgtcggcccc aatggctgcg gcaagtcgaa cgtctcggat
     2521 gcggttcgct gggtgctggg tgaacagtcc gcccgcctgc ttcgcggcgg caagatggaa
     2581 gacgtcatct tccagggctc ggcgaatcgg cgccctgtca acgtgaccga agtcgcgctg
     2641 ttcctcgaca acaccgacgg cgccctaccc attgcctacc aggaagtcgt cattgcccgc
     2701 cggctctcgc gatcggggca aagcgactac ctgctcaacg gcacagtcgt gcggctgcgc
     2761 gacatccagg acctgctgcg tgggaccggg ttgggcagtg atgccggggt tgtgatcgag
     2821 gccgagatga tcaagctgct tctgtccgac cgggcggatg agcggcgctc gctgttcgag
     2881 gaagcggccg ggatcgggct ctaccgtgac cgcaagcatt ccaccgagcg ccgactggag
     2941 gaaaccgcag cggacctgca gcgggtcgag gacctgttga acgaggtaca gtctcagatc
     3001 cgaagcctcg cccggcagaa gggcaaaacg gagcgctccg ccaagctcct ggaagaaaaa
     3061 ttcgcggtgc aggtcaccct cgcccgacgt cacctcgatc ggcttgcgga acggaccgac
     3121 ggactcgaag cccgtttcaa ccagctcagc gagctgttac caactgagcg ggagggggta
     3181 ggacgagccg acgagcgtcg cgaagaggcc gcgcgcgcaa gaaccgccgc ccaggtacac
     3241 cggaccgaga ttgcgcgccg tcggggtgcc gtccgggtgg aggtcgagaa gctggatggc
     3301 gacctggcgc tcgccgctga gcgactcggc acggccacca gtcggcgtac gaaggcgcag
     3361 gaggagagga cccagacgga gctgcgggcg gcgctcgctc taaccgagcg tgacgctgcc
     3421 gccgccgacc gcaccgccgc ggaggccgag catgcgaaca tcaacgtcga agtcgccagc
     3481 cgcgccgacg acgaggagag cgggcgccag aagctggtgg gccagcggac tgccgtgcga
     3541 caactggaag aagaccttca gtcgagagcc caggcggttc gctcattgga aggtgagcgg
     3601 acggcactcg aaggtgagct ggcatcgctc cgcgatcgtg cggcccaggc cgcggcgcat
     3661 cgtgaggggc tccggtcaga gctcgaggca gcccagcgcc gccgcgacca cgcgatcgaa
     3721 caggccgctt
//
LOCUS       DASRJQ010000431         3754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3111439, whole genome shotgun sequence.
ACCESSION   DASRJQ010000431 DASRJQ010000000
VERSION     DASRJQ010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3111439"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VFB89_09735"
     CDS             <1..781
                     /locus_tag="VFB89_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27627.1"
                     /translation="GKLMHLFLAREPDMSAFAHLHPTTTDSVHWINPVPDLPAGRYRV
                     FGDIVHESGFARTLVTTLDVPAKAGDASRDSRAKPSPVLGEDDAVYVGGAAAGDTARL
                     ANGGTVVWERGRVPLTAGAPAPLRFSIRDAAGGLATLEPYLGMAAHAVVLRDDGSVFI
                     HLHPMGTISAASQATFAIRQQGDTATGAIGRRIAADDSAMAGMSHAITAGTVSFPYAF
                     PRPGTYRIWVQAKLAGKVETAAFEARVREKAGKGKENNRLN"
     gene            complement(1078..1692)
                     /locus_tag="VFB89_09740"
     CDS             complement(1078..1692)
                     /locus_tag="VFB89_09740"
                     /inference="COORDINATES: protein motif:HMM:NF021511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HZM27628.1"
                     /translation="MNDTQPGGYEITLLVIILAVLVWSGIHPSDRFTWLMEVFPVLIA
                     VPVLILTYRRFPFTPLAYSLIAVHACILMLGGKYTYAEVPLGFWMERAFGFARNHYDR
                     IGHFAQGFVPAIVAREILSRQSPLRGSRWLPFLVVCFCLALSACYEFIEWWTALATGT
                     AADAFLGTQGDPWDTQWDMFFAFIGAIVAVVTLSGVHDRELRKL"
     gene            complement(1708..2622)
                     /gene="rnz"
                     /locus_tag="VFB89_09745"
     CDS             complement(1708..2622)
                     /gene="rnz"
                     /locus_tag="VFB89_09745"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HZM27629.1"
                     /translation="MLSITLLGTGAACPSVERNVAALALAREGETILFDCGEGTQRQM
                     MRYGTSFAFREIFFTHYHADHMLGVTGLMRTMGLQDRSAGVTLYGPRGATKLLGAAIE
                     LGIERNRFPIEIVELQAGDRIRRDEYDIVIFPTDHRADTVGYAVVEHTRLGRFNPERA
                     HELGIPEGPLWGRLHRGESVELPDGRRIGPAELVGDPRRGRTVVYTGDTRPSPAVVEA
                     ARYADLLVHDATFGEDERARAVETGHSTAAEAAEVAREADVRQLVLTHISPRYTRDAP
                     ELLAEAKAVFPNSIVARDGLTIEVPFTD"
     gene            complement(2804..3406)
                     /locus_tag="VFB89_09750"
     CDS             complement(2804..3406)
                     /locus_tag="VFB89_09750"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZM27630.1"
                     /translation="MPTFDKNRQLSARFDQAFSYASQLHSSQTRKGSRTPYIAHLLGV
                     AALVLEDGGSEDEAIAALLHDSIEDHPREGHTEEEIRLLFGERVLKIVLGCTDAFSHP
                     KPPWKKRKLDYLDHLSSAPPDIRRVSAADKLHNARAVLHDYRHQGERLWRRFNAGKKD
                     QLWYYRSLVDVLKKGRRRPSALVRELERVVFELDQAVNGR"
     gene            complement(3408..>3754)
                     /locus_tag="VFB89_09755"
     CDS             complement(3408..>3754)
                     /locus_tag="VFB89_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27631.1"
                     /translation="NHGAARELTQTMLSLQRLSARLDTTLANPSIGSLLHNTDTLTQN
                     LSRLSQQLTVASTRLDTLLSGVNQGRGTIGKFATDTTLYYELRDLTAAMKGLVADLRK
                     NPGKLGVTVKAF"
BASE COUNT          684 a         1206 c         1172 g          692 t
ORIGIN      
        1 cggcaagctg atgcacctgt tcctggcgcg cgagccggac atgagcgcgt ttgcccacct
       61 tcatccaacg acgaccgact cggtgcactg gatcaacccg gtgccggatt taccggcggg
      121 acgttatcgg gtgttcggtg acattgtgca cgagagtggg ttcgctcgca cgctcgtgac
      181 cactctggac gttcctgcga aggctggtga cgcctcccgc gattcaagag cgaagccatc
      241 gcccgtgctc ggcgaagacg acgcagtcta tgtgggcggc gcagcagcag gagacacggc
      301 caggctcgct aacggaggaa ccgtcgtgtg ggagcggggc agagtgccat taactgcagg
      361 cgccccggct ccgctccgct tctcgatccg cgatgcggcc ggcggtctcg cgactttgga
      421 gccgtacctc gggatggccg ctcatgctgt ggtgctgcgt gatgacggca gcgtgttcat
      481 tcaccttcac ccaatgggca cgatatcggc cgcgtcgcag gccactttcg cgatccgcca
      541 gcagggcgat accgcgacgg gagccatcgg ccggcgaatc gccgccgatg attccgccat
      601 ggccggcatg tcgcacgcga ttacagccgg taccgtctcc ttcccttatg cctttccccg
      661 accaggaacc tacaggatct gggtgcaggc caaactcgcg ggaaaagtgg agacggcggc
      721 gtttgaggca agagtgcggg agaaggcggg aaaggggaaa gagaataatc ggcttaattg
      781 accgacttat ggcgtgctta gtttcagccc ggtttcctga ttccttcctc tccctttccc
      841 tttttcccat tctgcccttc tcccgcccta tgggtctcct ccttccactg ctgacccgta
      901 agcgacacca aagtgcctcg tgtaggtccg cgacggcccg aagctatagc cctccgcgat
      961 gttgagctcg acagagaggg atgcgcgctg aagctgcgca aacaacgccg aggccccttt
     1021 cgcgtttctt cccttctccc tttcctcctc ggctggcagc gggcctgcca cgccgcgtca
     1081 gagcttacgc aactcgcggt cgtgcacgcc gctcagcgtc accactgcga cgatcgcgcc
     1141 gatgaaggcg aagaacatgt cccactgcgt atcccaggga tcgccttgag tccccaggaa
     1201 agcgtcggcg gcggtgccgg tcgcaagggc cgtccaccac tcgatgaact cgtaacacgc
     1261 gctcagagcc aggcagaaac aaacgaccag aaatggaagc cagcggctcc cacgcagcgg
     1321 cgactgccgg ctgagaatct cccgagccac gatcgccggc acgaagccct gagcgaagtg
     1381 accgatgcga tcgtagtggt tgcgcgcaaa tccgaacgcc cgctccatcc agaagccgag
     1441 cgggacctcg gcgtaggtat acttgccgcc gagcatcaga atgcaggcgt ggaccgcgat
     1501 caagctgtac gccagaggag tgaagggaaa gcgtcggtaa gtcaggatga gaacgggaac
     1561 ggcgatcagg accggaaaca cttccatcag ccaggtaaag cggtcgctgg gatggattcc
     1621 cgaccagacc agcacggcga ggatgataac caggagggta atctcgtaac cgccgggctg
     1681 agtgtcgttc atcgaacctc tcccgaatca atcggtaaac ggcacctcga tcgtgagccc
     1741 gtcacgcgca acgatggaat tgggaaagac cgcctttgcc tcagccagca actcgggcgc
     1801 atcgcgagtg taacgcgggc tgatgtgggt cagcaccagt tgacggacgt ccgcttcccg
     1861 cgcgacttcg gcggcctcgg ctgccgtcga gtgtccggtc tcgaccgcac gagcccgctc
     1921 gtcctcgccg aatgtcgcgt cgtgcaccag cagatcggcg taacgcgctg cttccaccac
     1981 cgcgggtgac gggcgcgtgt ccccggtgta caccacggtc cgcccacggc gcgggtcacc
     2041 caccagctcg gcaggcccga tccgccgccc gtcgggcagc tccacgcttt cacctcggtg
     2101 caggcggccc cagagcggcc cttcgggtat gccgagctcg tgggcccgct ccggattgaa
     2161 gcgcccgagc cgggtatgct cgaccacggc gtagccgacc gtgtccgccc ggtgatcggt
     2221 tgggaagatc acgatgtcat actcgtcacg ccgaatccgg tcacccgctt gcagctcgac
     2281 aatctcgatg gggaaccgat tacgctcgat gcctagctcg atggccgccc cgagcagctt
     2341 ggtggcacca cggggaccgt acagcgtcac tcctgcgctg cgatcctgca aacccatggt
     2401 ccgcatcagt ccggtgacgc cgagcatgtg gtcggcatgg tagtgcgtga agaagatctc
     2461 cctgaaagca aagctggtcc cgtagcgcat catctggcgc tgggttccct ccccgcaatc
     2521 gaagagaatg gtctcgccct ctcgcgcgag ggccagcgcg gcgacattgc gttccacgct
     2581 cgggcacgca gcgccggtgc cgagcagggt gatggatagc atggccggga aggtagctgg
     2641 aacacggtga acggtgaacg gtaaacggtg aacgggagcc cccaggagcg ctgagcggga
     2701 actcctagga gtggcgagcg ggcagtcccg cgacgccgct cccagccccg gttcgccgtt
     2761 ctgaagaact cccgtttacc gtttaccgtt caccgttcac gctttacctg ccattgaccg
     2821 cctggtccaa ctcgaacacc acccgctcca gctctcgcac cagcgcgctt ggccgacggc
     2881 ggcccttctt gagcacatcc accaggctgc ggtaatacca gagctggtct ttcttaccgg
     2941 cattgaaccg gcgccagagt cgctcgccct gatggcgata gtcatggagg acggcgcggg
     3001 cgttgtgcag cttgtcggcg gcggagaccc gccggatatc aggtggggcg gaggacagat
     3061 ggtccaggta gtcgagcttc cgttttttcc agggcggctt cgggtgcgaa aacgcgtcgg
     3121 tgcagcccag gacaatcttg agtacccgct cgccaaagag cagcctgatc tcctcctcgg
     3181 tatgcccttc ccgcggatgg tcttcgatgg agtcgtgcag cagtgcggcg atcgcctcgt
     3241 cctcgctgcc gccgtcctcc agcaccagcg cagcgacgcc gagtaagtgc gctatgtacg
     3301 gcgtccggct tccctttcga gtctgcgaac tgtggagctg gctggcatag ctgaaggcct
     3361 gatcgaaccg ggctgagagt tgacggttct tgtcgaaggt cggcatgtca gaacgccttt
     3421 acggttactc ccagcttacc cgggttcttc cgaaggtcgg ccaccagtcc cttcattgca
     3481 gctgtcaagt cacgcagctc gtaatacaac gtggtatccg tcgcgaactt tccgatggtc
     3541 ccccgccctt ggttgacccc gctcaggagc gtatccagtc gggtactcgc aaccgtcagc
     3601 tgctgggaca gccggctcaa attctgggtg agcgtgtcag tgttgtgtag cagcgatccg
     3661 atcgacggat tggccagtgt ggtgtcgagc cgcgcgctca accgttgaag cgaaagcatg
     3721 gtctgggtga gctctcgggc ggctccgtgg tttt
//
LOCUS       DASRJQ010000432         7607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2565953, whole genome shotgun sequence.
ACCESSION   DASRJQ010000432 DASRJQ010000000
VERSION     DASRJQ010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7607
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2565953"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="VFB89_09760"
     CDS             complement(<1..638)
                     /locus_tag="VFB89_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27632.1"
                     /translation="MRSTTRRRLLLPALTATAALALAACSGDSTGPATQTSLTQQDAN
                     AAADVIVGDFSDQSDGATTTSSGASFSLGAPSFGISQSPSARPAGAWYSTACSPPPTV
                     TTNGSTTTYVFDHCAISRLMPLETLTRTGEVDVTRDVGSRVVVFKNFDRSWERISFRT
                     GKTITTSATQNGTRSIAGDATTLEFHAYGTGDPATSLFQTDFVHEDQSTSRH"
     gene            complement(777..2012)
                     /locus_tag="VFB89_09765"
     CDS             complement(777..2012)
                     /locus_tag="VFB89_09765"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HZM27633.1"
                     /translation="MLLAHLEEQAFAAPLVGAPNSRRIGAEVELIPVDAATGRPCLLE
                     NGPGPAVLPFLRRFGARQGWVEQRTAKGVVCFAVATGGSVSFEPGGQIEYASSPSRSA
                     TTLINRLRDVVLPLRAAAAAEGISLLTVGIDPRNPVEQVALQLSCDRYTRMAAHFARI
                     GPSGARMMRQTAAFQISVDFEDQPLERWRLLNCLAPFVTAIFANSPMYAGEDTGHRSF
                     RAHCWRMLDLQRTGLPYDPQRPIEAYLEFALAAPAILLPTVGGRCAPFDYWLSRANPS
                     LDEWETHLTTLFPDVRPRGHLELRSMDAVDPAGYPAALAMVAGLVYQPDARREALDLL
                     PAPDLGLLERAGRLGLTDPELARTASDLFQLALTGCAALGTKFLHPAHLEEARAYFDH
                     YTRLGRSPADEFEACAVAA"
     gene            complement(2030..2992)
                     /gene="egtD"
                     /locus_tag="VFB89_09770"
     CDS             complement(2030..2992)
                     /gene="egtD"
                     /locus_tag="VFB89_09770"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HZM27634.1"
                     /translation="MPKVNTTAIAQPNPRMLADVAAGLSGSQKELPPKYFYDHRGSKL
                     FEEITRLPEYYLTRAERALLEQWMPHLIRSLGSRTLVELGAGSAEKSRIILNAMRTAG
                     TAEVYVPIDVSAAFLRETADQIRAEYPGLTVTPGVADISEELNVPRGLASPVLHAFLG
                     STIGNFHSEAAIKLLRRIRSIMWAGDRFLMGADLRKDVGLIEAAYNDSQGVTAEFNRN
                     MLRVLNHELGADFDLSRFVHRAFYNRDAHRIEMHLISTRPQIVNLPGIGPIKFVAGES
                     IRTEISCKYDIPSLASLFSTAGLRLESWKTDPERRFALVVAKPV"
     gene            complement(2985..4328)
                     /gene="egtB"
                     /locus_tag="VFB89_09775"
     CDS             complement(2985..4328)
                     /gene="egtB"
                     /locus_tag="VFB89_09775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HZM27635.1"
                     /translation="MTHIVPARPADTNATADQLRATLAKELTEARRRTLWLVAPLSDE
                     DLHRQHDPLMSPILWDLGHIAAFEDLWLTRNLTGRIEFSEMPGLYNPFEHPRRVRGTL
                     ALPGLGEARETMDEIRTRALARLETADFRNGPRLLLNGFVYRMVLQHEYQHDETILQT
                     LQLKRGSPYRPVERRPLPPPIDIHRPGEMVFFPGGSVEVGTNDRSAAYDNERPRHQVN
                     IEPFWIDVTPVTNGQFLEFVEAGGYRRRELWNDEGWQWLSEADVVAPKHWEKTLGGWT
                     TRTMDLIRPLDPARPVCHVCYHEADAYAKFAGKRLPTEAEWETAASWDPSAGVGRRYP
                     WGDEPWNPELANLDQSAFDTAPVGAYLRNVSPIGCYGMIGDVWEWTSSDFGGYPGFEP
                     FPYAEYSEAFFGSEYKVLRGGSWATRPGAVRATFRNWDYPIRRQIFSGFRCARDA"
     gene            complement(4345..5742)
                     /locus_tag="VFB89_09780"
     CDS             complement(4345..5742)
                     /locus_tag="VFB89_09780"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HZM27636.1"
                     /translation="MANVQHYDALIIGAGQAAVPLARTLAQSGRRTALVERVHVGGTC
                     INEGCTPTKTMVASARVAYLARRGADYGVGTGQISIDLARVRERKQAVVDSFRNGSQR
                     RLESTANLDLIFGDARFTGEKQLEIRPAKDRRSPQTVTADLVFINSGGRPARPALQGI
                     DQVSPLDSTSIMELSDVPEHLVILGGGYIGLEFAQMFRRFGSKVTVIQRNAQLLPNED
                     ADVADTVREILVEDGVEVVLQAESRCVAADGPGIRLGISTSEGERIIRGSHLLLAAGR
                     TPNTDSLNCGAAGIALDDKGFIKANSRLETSASDVYALGDVKGGPQFTHISYDDFRII
                     RSNLLEGGNATTDHRVLPYTVFIDPQLGRIGLSEKDARKQGRNIRVAKMPMSNVARAV
                     EMDEPRGFMKAVVDADSGKILGAAVLGVEGGEIMSQLQVAMMGGVTARALRDAIFAHP
                     LLAESLNNLFATLDN"
     gene            complement(6025..7128)
                     /gene="serC"
                     /locus_tag="VFB89_09785"
     CDS             complement(6025..7128)
                     /gene="serC"
                     /locus_tag="VFB89_09785"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HZM27637.1"
                     /translation="MSDRIYNFSAGPAVLPEPVLKQAQQDLWNIGGSGIGIMEHSHRG
                     KLFEKIVAEAEQDCRELAGISDRYRILFLQGGASLQFAMVPLNLLPRGATADYINTGT
                     WSEKAIKEAKLVGKSHEAATSADRKFSYVPSAQQIRYSERPAYVHFTTNNTIEGTQFK
                     IEPGVPSGVPLVADTSSDMFSRPIDVTKYGLIYAGAQKNLGPSGVVLVIIRDDLVEAG
                     PKDIPTMLQYRTHAGEHSLYNTPPTLGIYMIGRVFKWIRELGGLRAMAEQNAEKAKGL
                     YDFLDQSDFFRGTAETDSRSLMNVCFRAPNEDLEAKFVAEATKRGLDGLKGHRSVGGM
                     RASIYNAFPRQGVSALVNFMKEFEKVNRAAVPA"
     gene            complement(7131..>7607)
                     /locus_tag="VFB89_09790"
     CDS             complement(7131..>7607)
                     /locus_tag="VFB89_09790"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HZM27638.1"
                     /translation="NKGVRVGLDVFQNEPTAPTGTFDSAIAKEPGVYGTHHVGASTEQ
                     AQVAIAHEVIHIVQEYQHSGIVPNCVNRLARSSASHILAVRHRNRPGVLADVFRVLAD
                     RAINVEEGENIIYHGAHATLARIHLDGKPDVAALEAIRKGNEHVISVELTAIDGRG"
BASE COUNT         1350 a         2406 c         2479 g         1372 t
ORIGIN      
        1 tcgtgtcgcg aggtggactg gtcctcgtga acgaagtcgg tctggaacag actcgtcgcc
       61 gggtctcctg tgccgtaggc gtgaaactcg agtgtggtgg cgtcgccggc gatactgcgg
      121 gtcccgttct gcgttgcgga cgtggtaatg gtcttcccag tgcggaacga gatccgttcc
      181 cagcttcggt cgaagttctt gaagacgaca actcggctcc cgacatcgcg ggtgacatca
      241 acttcgccgg tccgagtcag cgtctcgagc ggcatcagtc gggaaatcgc gcagtgatcg
      301 aacacatagg tcgttgtgct accgttggta gtgaccgtcg gagggggcga gcacgctgtg
      361 gagtaccagg ccccggccgg ccgcgccgaa ggagactggc tgatcccgaa ggacggcgcg
      421 ccaagcgaga agctcgcgcc tgacgaggtc gtggtggcgc cgtcactctg atccgagaaa
      481 tcgccgacga tcacatccgc ggcggcattc gcgtcctgct gagtgagcga tgtttgcgtt
      541 gcgggacccg tagagtcgcc gctgcaggca gcaagggcca gagctgccgt ggcggtcagc
      601 gccggtagca acagacgacg ccgagtggtc gagcgcatcg gcctatcctc catctcggtt
      661 ggacatgcga acggttgggc gaactgctcc cgacggacgg tgccggccgg cagacgttaa
      721 atccaggtcc tgccgctgca actgcttgca gagtggagga agggacccct aaacggtcag
      781 gctgctaccg cgcaggcttc gaactcgtcc gccggcgacc ggccgagcct ggtgtaatga
      841 tcaaagtagg cccgggcctc ctcgaggtgg gccggatgga ggaacttggt ccccaatgcc
      901 gcgcaaccgg tcaaggccag ctggaacagg tcggaagccg tccgggccaa ctcaggatcg
      961 gtcaatccga ggcgcccggc tcgctccagc aaccccaggt cgggggcggg caggaggtcc
     1021 agagcctctc gcctggcgtc gggctgatag accagccctg cgaccatggc cagggccgca
     1081 gggtacccag ccgggtccac ggcgtccatg gagcgaagct ccaagtgccc tcttggtcgt
     1141 acgtcgggaa agagggttgt gaggtgagtt tcccactcgt cgagggacgg gttggcgcgg
     1201 gacagccagt aatcgaacgg ggcgcatcgc ccgccgacgg tcggcagcag gatggcgggg
     1261 gcggccaggg cgaattcgag gtacgcctcg atcggacgct gcggatcgta cggcaagccg
     1321 gtgcgctgca gatcgagcat gcgccagcag tgggcgcgga agctccggtg cccggtgtcc
     1381 tcgcccgcgt acatcggaga gttggcaaaa atggcggtga cgaagggcgc caggcagttc
     1441 aacagccgcc aacgctccag gggctgatcc tcgaagtcca cgctgatctg gaacgcagcg
     1501 gtctgtcgca tcattctcgc tccgctcggg ccgatccgcg cgaaatgggc cgccatgcgg
     1561 gtgtagcggt cacaggaaag ctgtagtgct acctgctcga ccgggtttcg tgggtcgatg
     1621 cccacggtaa gcagcgaaat gccctcggcg gcggcggcgg cccgcaacgg cagaaccaca
     1681 tcacggaggc ggttgatcag ggttgtggca gagcggctcg gagagctggc atactcgatt
     1741 tgacctcccg gctcgaagct cacgcttcca ccggtggcca cggcaaagca cacaacgccc
     1801 ttggcggttc gctgctccac ccagccctgt cgcgcaccga accttcggag aaacggtaac
     1861 acggcgggcc cgggaccatt ctccagcaga cagggccgtc cggttgcagc gtccaccggg
     1921 atgagctcca cttccgcgcc gatgcgacgc gagttcggag ctcccaccag aggagcggca
     1981 aacgcctgct cctccaggtg cgccagcagc atcggacggc tcaaccgatt cataccggct
     2041 tcgctaccac cagcgcgaat cgccgctccg gatcggtctt ccacgactcc aggcggaggc
     2101 ccgccgtcga gaacagcgag gccaagctgg gaatgtcgta cttgcagctg atctctgtgc
     2161 ggatggattc gccggccacg aacttgatgg gtccaatacc gggcagattc actatttgcg
     2221 gccgagtcga gatcaggtgc atctcgatcc gatgcgcgtc gcgattgtaa aacgctcgat
     2281 gaacgaacct gctcaggtcg aagtcggcgc ccaactcgtg attgagcacg cgcagcatgt
     2341 tgcggttgaa ctccgccgtc accccttgcg aatcgttgta cgccgcctcg atcaagccca
     2401 cgtccttgcg gaggtcggcg cccatcagaa accgatcgcc tgcccacatg atgctgcgaa
     2461 tccgccgcaa gagcttgatt gcggcctccg agtggaagtt gccgatcgtg ctgcctagaa
     2521 aagcatgcag gaccgggctg gcgagccccc gaggcacatt cagctcctcg gaaatgtccg
     2581 ccacgcccgg cgtgaccgtg aggccggggt actcggcccg aatctggtcg gcggtttccc
     2641 gcaggaaagc ggcgctcaca tcgatgggga cgtagacctc cgccgtaccc gccgtacgca
     2701 tcgcgttcag aatgatgcgg gacttctcgg cgctgccggc gcccaactcc accagcgtgc
     2761 ggctgcccag cgagcggatc agatggggca tccactgttc cagcagggcg cgctccgccc
     2821 gggtgaggta gtactcggga agacgggtta tctcctcgaa gagcttcgac ccacgatggt
     2881 cgtagaagta cttgggcggc agttcctttt gggagccgga caggccggca gccacgtcag
     2941 ccagcatgcg cggattgggt tgcgcaatgg ctgtggtgtt gaccttaggc atcgcgagca
     3001 caccggaagc cgctgaagat ctggcggcgg atagggtagt cccaattgcg gaaggtggcg
     3061 cgcacggcgc cagggcgcgt tgcccacgac cctccgcgaa gaactttgta ttctgagccg
     3121 aagaaggcct cggagtattc ggcgtaggga aacggctcga aaccggggta ccctccgaag
     3181 tcgctggacg tccactccca tacgtcgccg atcatcccgt aacagccgat cggggagaca
     3241 tttctgaggt acgctcccac tggtgccgtg tcgaacgcgc tctgatccag gttggccagc
     3301 tcagggttcc agggctcgtc accccacgga taccgtcggc ccacgccggc cgatgggtcc
     3361 caggacgcag cggtttccca ttccgcttca gtgggaagtc gcttacccgc gaactttgcg
     3421 taggcgtcgg cctcgtggta gcagacgtga cagactggac gcgccgggtc gagcgggcgg
     3481 atgaggtcca tcgtgcgagt ggtccagccg ccgagggtct tctcccagtg cttaggcgcg
     3541 acaacgtcgg cctccgatag ccactgccaa ccctcgtcgt tccacaactc tctacgccga
     3601 tagccgccgg cttcgacgaa ctccaggaat tgaccgttgg tcaccggcgt cacgtcgatc
     3661 cagaacggct cgatgttcac ctgatgacgc gggcgctcgt tgtcgtaggc ggccgagcga
     3721 tcgttggttc caacctccac cgaaccgccg gggaagaaga ccatctcacc gggacggtgg
     3781 atatcgatcg gaggcggaag cggacgacgc tctacggggc gatacggtga gccgcgcttg
     3841 agctggaggg tctgaaggat cgtctcatca tgctggtact catgctgcag aaccatccgg
     3901 tacacaaacc cattcaacag cagccgcggc ccgtttctaa agtcagcggt ctcgagccgg
     3961 gccagggcgc gggtccggat ctcatccatg gtctcccggg cctcgccgag accgggaagg
     4021 gcaagcgtgc cgcgaacgcg gcgggggtgc tcaaaggggt tatacagccc cggcatctcg
     4081 ctgaactcga tccgaccggt gaggtttcgc gtcagccata ggtcttcgaa ggcggcgatg
     4141 tgcccgagat cccaaaggat cgggctcatc aggggatcgt gctgccggtg cagatcctcg
     4201 tcgctcagcg gcgctaccag ccacagcgtc cggcgccgtg cttccgtcag ctcctttgcc
     4261 agggttgcgc gaagctggtc ggccgtagcg tttgtgtcgg ccgggcgggc gggtacgata
     4321 tgcgtcatcg cggctccgaa gggctcagtt atcgagcgtc gcgaacaagt tgttgagaga
     4381 ctcggccagc agcgggtgtg cgaagatcgc gtcgcgcagc gcccgggcgg tcaccccgcc
     4441 catcatcgcg acctgtaact gcgacatgat ttccccgcct tcgacgccga gcactgccgc
     4501 gcccaaaatc tttccactgt ccgcgtcgac gacggctttc atgaacccac ggggttcgtc
     4561 catctcgacg gcccgggcga cgttgctcat cggcatcttg gcaacccgga tgttgcgccc
     4621 ctgcttccgg gcatccttct cactcaggcc gatgcggccg agctgcgggt cgatgaacac
     4681 cgtatagggt agcacgcgat gatccgtggt cgcgttgccg ccctccagca ggttgctccg
     4741 gatgatgcgg aagtcgtcgt aggagatgtg agtgaactga gggccgccct tcacgtctcc
     4801 cagcgcgtag acatcggaag cgctcgtctc caggcgactg ttggctttga tgaagccctt
     4861 gtcgtccagt gctataccgg ccgctccgca gttcagcgaa tcggtgttgg gagtgcggcc
     4921 ggcagcgagg agcaggtgcg agccccggat tatccgctcg ccctccgagg tcgagattcc
     4981 caaacggatg cccggtccgt ccgcggcgac acaccgggac tcggcctgga gcactacttc
     5041 gacgccgtcc tctaccagaa tctcccgaac agtgtcggcg acgtcggcat cctcattagg
     5101 aagaagctgc gcgttgcgct gaattacggt gaccttgctg ccgaaccggc gaaacatctg
     5161 agcgaactcg agaccgatgt agccgccacc gagaatgacc agatgctcgg gcacatcgga
     5221 cagctccatg atcgaggtcg aatcgagcgg cgacacctga tcgattccct gcagcgcggg
     5281 ccgggccggg cggccaccgc tgttgatgaa caccagatcg gcagtgactg tctgcgggct
     5341 tctcctgtcc tttgcagggc ggatctccag ctgcttctcg ccggtaaacc gggcatcccc
     5401 gaaaatcaga tcgaggttgg cagtggactc gagccgccgc tggctgccgt tgcggaagct
     5461 gtccactacc gcttgcttgc gctcccggac ccgcgcgagg tcgatactga tctgtccggt
     5521 tccgacaccg tagtcagcac cgcggcgggc gagataggcc acgcgtgcgc ttgccaccat
     5581 cgtcttggtc ggagtacagc cctcgttgat gcaggtcccg cctacgtgca cgcgctccac
     5641 gagcgccgtc cgtcggccgg attgagcaag agtccgagcc agcggcactg ccgcctgtcc
     5701 agcgccgatg atgagcgcgt cgtagtgctg cacatttgcc attggggggg cctcgcgcgt
     5761 cgtgaccgga cgtctatagg agaagacaac ggggaaacga ggcctagagt caaaaggttc
     5821 cttcggtcag gtcatggccg acctgaaccc cggaaccgct atcaaaggct tatacggcct
     5881 gaaggtgggt cgggcaagac cgggcagcca tgacctccgt caccagctac ccgactgtta
     5941 cccgggacta aggaagacaa acgaggcccg ggccgccgcg ggggcccggg cctaagtcac
     6001 tgctccagcg tagcttccgg tgattcaggc ggggacagcg gcccgattca ccttctcgaa
     6061 ctccttcatg aagttgacca gcgccgagac gccctggcgg gggaaggcat tgtagatgct
     6121 tgcccgcatg ccgcccaccg agcggtgccc cttgagccca tcgagccccc gcttggtcgc
     6181 ctcggccacg aactttgctt cgagatcttc gtttggggcg cggaagcaca cattcatgag
     6241 agagcgactg tcggtctccg cggtgccgcg gaagaagtcg ctctggtcca ggaaatcgta
     6301 gaggcccttg gccttctccg cgttctgctc ggccatggcg cgcaaaccgc ccagctcgcg
     6361 gatccacttg aagacacgac cgatcatgta aatgcccagc gtcggtggcg tgttatagag
     6421 cgaatgctcg ccggcgtggg tccgatactg gagcatggtc gggatatcct tgggtccagc
     6481 ttcgaccagg tcgtcccgaa tgatgacgag caccacgccc gaggggccca gatttttctg
     6541 agcgccggcg tagatcaggc cgtacttggt aacgtcgatc ggccggctga acatatcgct
     6601 cgacgtatcc gcgaccagcg gaacacccga cgggacgccc ggctcgatct tgaattgggt
     6661 tccttcgatc gtgttgttgg tcgtgaagtg cacgtatgcc ggccgctccg agtaccggat
     6721 ctgctgggcc gaagggacgt agctgaactt ccgatcggca ctcgttgccg cttcgtgcga
     6781 cttgcccacg agcttcgctt ccttgattgc cttctccgac caggtcccgg tgttgatgta
     6841 gtcggccgtg gctcccctgg gcagcaggtt aagcggcacc atggcaaact gaagcgacgc
     6901 cccaccctgc aggaacagta tcctgtagcg atctgagatc ccggccagct cgcgacagtc
     6961 ctgttccgcc tccgccacga tcttctcgaa cagcttgccg cggtgactgt gctccatgat
     7021 gccgatcccg ctgccgccga tgttccagag atcctgctgc gcctgcttca gaaccggctc
     7081 cggcagcacg gccggaccgg cgctgaagtt gtagatgcgg tccgacatga tcagcctctc
     7141 ccgtcgatcg cggtgagctc cacgctgata acgtgctcgt tgcccttgcg aatggcctcg
     7201 agcgccgcga catccggctt gccgtccagg tggatgcgcg cgagcgtcgc gtgcgcgccg
     7261 tggtagatga tgttctcgcc ctcctccacg ttgatcgcgc gatcggcgag cacgcggaag
     7321 acgtctgcca gaacgccggg ccggttgcga tggcgcacag cgagaatgtg gctggccgag
     7381 ctgcgtgcca gccggttgac gcagttgggg acgatccccg aatgctggta ctcctggacg
     7441 atatgaatca cttcgtgcgc gatggccacc tgcgcctgct cggttgaggc gcccacgtga
     7501 tgggtgccgt agacgccggg ctccttcgcg atggcgctgt cgaaggttcc ggttggggcg
     7561 gtgggctcat tctggaagac gtcgaggccc acccgcacgc ctttgtt
//
LOCUS       DASRJQ010000433         7189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1588861, whole genome shotgun sequence.
ACCESSION   DASRJQ010000433 DASRJQ010000000
VERSION     DASRJQ010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7189
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1588861"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VFB89_09795"
     CDS             <1..376
                     /locus_tag="VFB89_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27639.1"
                     /translation="FFMALQGEDSALSSVAYRTYVRTASVALKRISIVPNDVVLQNDP
                     VPLAITLLLEANAPAGGQPVALATERGSVELPLAVVPEGTDNVTVTGQLLPFYGPGDT
                     TQTVTASTSVNRLDFDILIHKI"
     gene            381..1073
                     /locus_tag="VFB89_09800"
     CDS             381..1073
                     /locus_tag="VFB89_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27640.1"
                     /translation="MLTITNVEGAQRVDALLPGTGTNNRLFVFTGTAIVGWRAPDDDQ
                     LRSDQAELDISALHDNQLFNPRSDLAMVSAWPASLHATSDADQVTWAVDKATGEIAPD
                     GHLLAKFSLALQGASGAFSRLAYEVLVQALSVKVAGISAYPPVVRLAGQPVTLYVSLL
                     LDQAVAPPGATIRLSHNIGDVTLPPEVQVSGNSTMITGTVGLGFPKGSQMITLTAAND
                     ISQASTDFTVIA"
     gene            1089..1493
                     /locus_tag="VFB89_09805"
     CDS             1089..1493
                     /locus_tag="VFB89_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27641.1"
                     /translation="MSQQIVVPLGGSPTARFVTVAGTVDAEGIDTSHNTTLQRFTLEF
                     NLSDLHPTALPPPLSGYSLRASAQAGLTSINNSDDAPEWTWAVDGARTIIREGKLVLV
                     VDLAGQGGPDSEIVYHDAVLSSLTYQVSVLVS"
     gene            1634..2992
                     /locus_tag="VFB89_09810"
     CDS             1634..2992
                     /locus_tag="VFB89_09810"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZM27642.1"
                     /translation="MTVTAPTAVPEIVPEHVHTQEFHTPKAVRRGILAGVVGNMLEWY
                     DFALFGFFAQQLGAHFFPSGNPTVSLLAAFGTFAAGFLMRPVGGALFGWIGDRFGRKQ
                     ALIGSVLAMAFPSFLIGCLPDAKTIGIAAPIILILLRMLQGVAVGGEYMASAVFLVEG
                     SEPGRRGYMGSWGPFGASAGTLLGSAAGAIVNAAMTPEQVMAYGWRIPFLVGILVAFG
                     GLVIRRHYVERVPQQDTSEFPLGEAFRDHWKTLLHLVGLTTGISVGFYTTFVYSATWL
                     EQTVSVPARTALEINTVAMMLLLVIIPFAGIASDRIGRRPVLALASGGLMLLAYPLMT
                     LMAQGRPASIFAGQVGLALLVGGNGAVLPAAMAELAPWRVRCTVLSVGYNISLAILGG
                     TTPVVAAWLVSRTGFHLAPAVYLALASAVTFVAALLLPKTARHRMTKEFAALRPRASR
                     RS"
     gene            complement(3014..3748)
                     /locus_tag="VFB89_09815"
     CDS             complement(3014..3748)
                     /locus_tag="VFB89_09815"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZM27643.1"
                     /translation="MTAENVSAGSMRDSTTSPTIEQLVAEPPKLHSAGGSLTSRWKLQ
                     DADLRYLDHILRPGMRTLETGAGISTVVFALKGTDHTCIVPDQGLVDRIKNFCAESAI
                     PLANVDFVVEHSEQALPRLTRQDYDFALIDGRHGFPAPFIDCYYVAGLLKVGGSVMID
                     DLHIWTIELLVEYLRADANWQLGRETWHTATFVKRTDDALRDEWTRQPFVVKRSLRKS
                     FLAKLRLLQRMARTRDFTPLRRLWGT"
     gene            3875..3956
                     /locus_tag="VFB89_09820"
     tRNA            3875..3956
                     /locus_tag="VFB89_09820"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3909..3911,aa:Leu,seq:tag)
     gene            complement(4119..5450)
                     /locus_tag="VFB89_09825"
     CDS             complement(4119..5450)
                     /locus_tag="VFB89_09825"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HZM27644.1"
                     /translation="MLLARQASIKLIIVAGGIALLFACEERGGASRARSDTTATAFPV
                     SAQPLGPIPPDDSLARPKSVQQVGVPIGLARAVMPRDNPQTPEKIALGERLFFDGRLS
                     ANGTVACASCHDPRLAFTDGRPVSVGIGNRLGQRNAPTVLNALYNKFQFWDGRTATLE
                     AQAALPIINPVEMGQPTLDSAVARIAAVPDYRDAFRRAFGHQPNGVDLVRAIASYERS
                     QLSFDAPFDHFMAGDSNAIDAAAKRGWVLFNTRGRCNKCHALTDTTSRPTTFTDDTFH
                     NIGIGILRHRVVPLAHQAEQSIARGDTIAIDRAAILTPMSALGRYLITRKDADIASFK
                     TPDLRNVLVTGPYFHDGSVTTLWDVVDHYNKGDGVQDPWLDEDIQPLALQENDIDDLV
                     ALMASLTSPSYRELAAKELTRQRQLSRTSRPQRDSARAFGRKPVQPKPPTP"
     gene            complement(5619..6287)
                     /locus_tag="VFB89_09830"
     CDS             complement(5619..6287)
                     /locus_tag="VFB89_09830"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HZM27645.1"
                     /translation="MKARVFSIALAVGLLQAPSVLHAQAGAAAQASPWFGRIAALGAF
                     YHSSATIATSGILLQGATATVSNNQTLTFDIGYDFTRNLFAMLMVGVPPKPTVTGEGT
                     VTSLGALGRVRYGPAILTGGYRIGTGAGLRPYVGAGAAYAIIVKDHDAAVSQLDVHNN
                     WGFVLQAGAEYSLNKALDLFADFKQLWLAVDANGLLSGGVPVKARVTLNPSLVSAGVK
                     FRFH"
     gene            complement(6284..>7189)
                     /locus_tag="VFB89_09835"
     CDS             complement(6284..>7189)
                     /locus_tag="VFB89_09835"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM27646.1"
                     /translation="QDSVRAGVAGAVLLIPKDFERHVLRREAAYVEAYADASYFLVYS
                     TALRAINGVVGTLSTGISVRRLQAAGLTSPAALRRARPATFVYWPLFNPLAGYGTFVV
                     PAVFILILQQTLLIGIGALRVAERREDESSQPLWAVLGGKLGATALIYVGHALFMFGV
                     VFTLYGFPMRASWLDLVLFLLPYFAATTLLGLAIAELFDRPESSTVALAAMGLPALFL
                     SGISFPAEVQAGWVRVLAVVLPSTFGIQGFLQLAEMGARLDQTLRAWGALWIQVVVYW
                     SLAWLVRRYKGKARNLGGLGLAHRA"
BASE COUNT         1259 a         2333 c         2242 g         1355 t
ORIGIN      
        1 cttcttcatg gccctgcagg gcgaagactc agcgctctcg agcgttgcct atcgcacgta
       61 cgtgcgcacg gccagcgtcg cgctgaagcg catttccatc gtgcccaacg acgtggtcct
      121 ccagaacgat ccggttcccc tcgcgatcac tctgttgctc gaggccaacg ccccggcagg
      181 tggtcagccc gtcgcgctgg caaccgaacg cggctcggtc gagcttcccc tagccgtcgt
      241 gccggaggga acggacaatg tcactgtcac cggtcagctg cttccattct acggccccgg
      301 tgacacgacc caaaccgtga ccgcctccac ctcggtgaac cggctcgact tcgatatcct
      361 gattcacaag atctgagaca atgctgacca tcacgaacgt cgagggtgcc cagcgcgtcg
      421 acgccctcct tcccggaacc ggcaccaaca accggctctt cgtatttacc ggcaccgcca
      481 tcgtcggctg gcgcgccccc gacgacgatc agctccgatc ggatcaggcg gagctcgaca
      541 tttccgcgct gcacgacaac cagctcttca acccacgcag cgatctggcg atggtgtcgg
      601 cgtggcccgc gagcctccac gccaccagcg acgcggacca ggtaacctgg gccgtagaca
      661 aggcaaccgg agagatcgct cccgatgggc atctcctcgc caagttcagc ctggcattac
      721 agggagcgag cggagcgttc tcgcgtctgg cgtacgaggt cctggtgcag gcgctcagcg
      781 tgaaggtggc gggcatttca gcgtatcccc ccgtcgtccg tctggcaggt caaccggtca
      841 cgctctacgt ctcactgttg ctggatcagg ctgtcgcgcc accgggggcg acgatacggc
      901 tttcacacaa catcggcgac gtcaccctcc ctcccgaagt tcaggtaagc ggcaatagca
      961 cgatgatcac cggaacggtg ggacttggct tccccaaggg ctcgcagatg atcaccctga
     1021 cggccgccaa cgacatcagc caagcctcga ccgacttcac agtgatcgcc tgacggaagg
     1081 agcgaaccat gagccaacag atcgtagtgc ccctgggcgg atcgcctacc gcgcgcttcg
     1141 tcaccgtcgc cgggacggtt gatgccgagg gcatcgacac gtcacacaac accaccctgc
     1201 agcgcttcac cctcgagttc aacctgagtg accttcatcc gacggccctc ccgccgccgc
     1261 tttcgggcta ctccctccgc gcgtccgcgc aagccggcct caccagcatc aacaacagcg
     1321 acgacgcacc agagtggacc tgggcggtcg acggggccag aaccatcatc cgcgagggca
     1381 agctcgtact cgtcgtagat ctcgccgggc agggtggtcc ggattcggag atcgtctatc
     1441 acgacgcagt cctctcgagc ctgacatacc aggtttccgt gctggtgtcc tgaccccggg
     1501 gcaccgactg gctctacgcc tctcgcaggg caagacgtag ctggcgctcc cctcttcttg
     1561 gtggcggcgc gagtgtccgc gtggtaacgt attgcgcacg cattccttct ctctcaccac
     1621 gtcggatgtc ccaatgaccg tcaccgcccc gacggcggtt ccggagatcg ttccggagca
     1681 cgtccacacc caggaattcc ataccccgaa ggccgtgcgc cgaggcattc tggccggcgt
     1741 ggtcggcaac atgctcgaat ggtacgactt cgccctgttt ggcttcttcg cccaacagct
     1801 cggcgcgcac ttctttcctt ccggcaatcc aaccgtctcg ctcctcgccg ccttcggcac
     1861 tttcgccgcc ggttttctca tgcggcctgt cggtggtgcg ctgttcggct ggatcggcga
     1921 ccgattcggc cgaaagcagg ctctgatcgg ctccgtgctc gccatggcgt ttccgtcatt
     1981 tctcatcggc tgcctgccgg acgcgaagac catcgggatc gccgcgccca tcatcctcat
     2041 cctcctgcgc atgcttcagg gcgttgcggt cggcggggag tatatggcct ccgccgtctt
     2101 tctggttgaa ggatccgagc cgggtcgccg aggctacatg ggaagctggg ggccgttcgg
     2161 cgcgtcggcc ggcacgctgc tcggctcagc tgcgggtgcc attgtgaacg ccgccatgac
     2221 gccggagcag gtgatggcgt atggctggcg gattcccttt ctggtcggga tcctggtcgc
     2281 gttcggcggg ttggtaatcc ggcggcacta cgttgagcgc gttccgcagc aggacacgtc
     2341 cgagttccca ctgggtgagg cgttccgaga tcactggaag accctgctcc acctcgtggg
     2401 tctcaccacc ggcatcagcg tgggcttcta caccaccttc gtttactccg ccacctggct
     2461 ggaacagacc gtgagtgtgc cggcccgcac ggcactcgag atcaacacgg tcgcgatgat
     2521 gctgctgctg gtcatcattc cgtttgcggg catcgccagc gaccgcatcg gccggcgccc
     2581 ggtgctagcg cttgcatccg gtgggctgat gctgctcgcc tacccgctca tgacgttgat
     2641 ggcacagggt cggcctgcct ccattttcgc tgggcaggtc gggctggctc tgctggttgg
     2701 tgggaatgga gcagtgctcc cggcggccat ggccgagctg gcgccatggc gggtccggtg
     2761 cacagttctg tccgtaggct ataacatctc gctggcgatc ctgggcggca ctacgcccgt
     2821 ggtcgcggcc tggctggtct cacgcaccgg ctttcatctc gcacccgcgg tgtatctcgc
     2881 tttggcatcg gcagtgacat tcgtggccgc cctgttgctt ccaaagacgg cccggcatcg
     2941 gatgaccaag gaatttgcgg cgcttcgacc gagggcatcg cggcgatcgt gatgatcgct
     3001 attggcgagc ggtttacgtg ccccacaacc tgcggagggg cgtgaaatcc ctggtgcgcg
     3061 ccatccgctg aagcaggcgc agcttggcca gaaatgattt tcgcaggctt cgcttcacga
     3121 cgaacggctg gcgggtccac tcgtcccgca acgcgtcatc ggtccgcttt acgaaggtcg
     3181 ccgtgtgcca ggtctctcgc ccgagctgcc agtttgcatc tgcgcggaga tactcgacga
     3241 gcagctcaat ggtccagatg tgaaggtcgt cgatcatgac cgaccctccg accttcagga
     3301 gacctgcgac atagtaacaa tcgatgaatg gcgccgggaa tccgtgccga ccgtcgatga
     3361 gagcgaagtc gtagtcttgc ctcgtcaatc tgggcagcgc ctgttccgag tgctcgacca
     3421 cgaagtcgac gttcgcgagc ggaatggccg actcggcgca gaagttcttg attcggtcca
     3481 ccaacccctg atccgggaca atgcaggtgt ggtcggtgcc cttcaaggcg aagaccacgg
     3541 tgctgattcc ggctcccgtt tccagcgtgc gcatacccgg ccgcagtatg tggtcgaggt
     3601 accgtaggtc tgcgtcctgg agcttccatc gggacgtaag cgatccgccg gcgctatgaa
     3661 gcttgggggg ctcggcgaca agctgctcga tggtgggtga ggtcgtggag tcgcgcatgg
     3721 agcccgccga cacgttttca gctgtcatcc agtccgcctc atgagtggcc tgcgagcttt
     3781 agtgtcgcct gtagataagt acggcacaat caatgtcaca accggcggac cttcccgagc
     3841 tttcagagcc ctaccgatta tcgttgatgt acttgcgaac gtggcggaac tggcagacgc
     3901 gcaggactta ggatcctgtc ccgcaagggg tccgggttcg actcccggcg ttcgcattcc
     3961 actctgcagc aacatcctag acgtttccca ttctcgcttt gtacaccaac tcctgttgcc
     4021 actccctgat cgggagctgc acacggcggg gtttctcgat cggctgagta cgtccccccc
     4081 agcgcgctcc gctcaagcgg ctgccgagga ggtttgggtc agggagtggg tggtttgggc
     4141 tgaacgggct tgcgaccaaa cgctctcgcc gagtcgcgtt gtggtcgcga cgtccgcgag
     4201 agctggcgtt ggcgagtcag ctccttggca gccagttccc tgtagcttgg actcgtgagc
     4261 gacgccatca gcgcgaccag gtcatcgata tcgttctcct ggagtgccag cggctggatg
     4321 tcttcatcga gccaagggtc ctgtactcca tcacccttgt tgtagtggtc cacgacgtcc
     4381 catagtgtcg tgacagaacc gtcgtgaaag tagggtcccg tcaccagcac gttgcggagg
     4441 tctggtgtct tgaacgacgc gatgtcggca tccttccttg tgatcaggta gcgcccaagc
     4501 gcagacattg gggtcagtat cgcagctcgg tcgatcgcga tcgtgtcgcc cctggcgatc
     4561 gactgctcgg cctgatgcgc cagcgggacc acgcggtgac gcaggatgcc gatcccgatg
     4621 ttgtgaaagg tgtcgtcggt gaaggttgtg ggccggctgg tggtgtcagt caacgcatgg
     4681 catttgttac agcgacctcg cgtgttgaac agcacccagc cgcgcttcgc agcggcgtcg
     4741 atcgcgttcg agtctcccgc catgaagtga tcgaaggggg cgtcgaagct cagctgcgac
     4801 cgctcataag acgcgatggc gcgcaccaga tcgactccgt tcggctgatg gccgaaggcc
     4861 cggcggaacg cgtcgcggta atccgggacg gccgcgatgc gcgcgaccgc agagtcgaga
     4921 gtcggctgtc ccatctccac gggattgatg atcggcagcg ccgcctgggc ctccagtgtg
     4981 gctgtgcgcc catcccagaa ctggaacttg ttgtagaggg cgttcagcac tgtcggcgcg
     5041 tttcgctggc cgaggcggtt gccgatgccg actgagaccg gtctgccgtc ggtgaacgcc
     5101 agtcgcggat catggcagct ggcgcacgct acggttccgt tggcggacag acggccatcg
     5161 aagaacagcc tctcaccgag cgcgatcttc tctggcgtct ggggattgtc tcgcggcatc
     5221 actgcgcgag cgagaccgat cgggacaccg acctgctgaa ccgacttcgg tcgagcgagc
     5281 gagtcgtccg ggggaatggg acccagtggc tgcgcgctca cggggaatgc tgtcgccgtc
     5341 gtgtcggatc gcgcgcgcga ggcgccgccg cgttcctcgc acgcgaagag gagtgcgatg
     5401 cctcctgcta cgatgatgag tttgatgctt gcctgcctag cgagcagcat gtcgcgtagc
     5461 ctcgcgaacg tcttaaccaa attccacgaa gctgatcatt ccgcaacatc cgcggcgttc
     5521 acgcccttct gattgcataa cccattgccg atcttgtata ccaacacctg ccgacctgcc
     5581 cggggcgagc gagctctcag agttcagggc ccgccggtct agtggaagcg aaacttgacc
     5641 cctgccgaaa ccagcgacgg attcaaggtc acccgagcct tcacagggac gccgcccgaa
     5701 agcaacccgt tcgcgtcgac cgcgagccaa agctgcttga agtcggcaaa caggtccaac
     5761 gccttattca aactgtactc cgctcccgcc tgcagcacga agccccagtt gttgtgaacg
     5821 tcgagctgcg agaccgcggc gtcgtggtct ttgacgatga tggcgtatgc tgcccccgcg
     5881 ccgacatagg gccgtaagcc cgcgccggtt ccgatgcgat acccacccgt caaaatcgcc
     5941 ggaccatatc gaactcgtcc aagagcaccc aatgatgtga cggtgccttc tccagtaacc
     6001 gtcggctttg gcggcacgcc caccatgagc atcgcaaaca ggttcctggt gaagtcgtag
     6061 ccgatgtcga aggtcagggt ttggttgttg ctgaccgttg ccgtggcgcc ctgaagcagt
     6121 atcccgctcg tggcgatcgt cgcactcgaa tgatagaatg cgccgagcgc tgcgattctc
     6181 ccaaaccagg ggctggcttg ggccgcggcg ccagcctgag cgtgtaggac gctcggagcc
     6241 tgaagcagcc caactgccag cgcgatcgag aagactctcg ctttcatgcc cggtgagcca
     6301 ggccgagccc gccgaggttg cgcgcctttc ctttatatcg ccgcaccagc caggccaggc
     6361 tccaatacac gaccacctgg atccagagcg ccccccaggc tcggagcgtc tgatccagcc
     6421 gcgcgcccat ctcggccagc tgcaggaacc cctgaatgcc gaacgtgctg ggcagcacga
     6481 ccgccagtac gcggacccag cccgcctgca cctccgccgg gaagcttatg cctgacagaa
     6541 agagcgccgg cagccccatc gcggcgagcg cgacggtcga ggactcgggc cgatcgaaca
     6601 gctcagcgat cgcgaggccg agcagggtcg tggccgcgaa gtagggcaga aggaagagca
     6661 caagatcgag ccacgaggct cgcattggaa agccgtaaag ggtgaacacc acgccgaaca
     6721 tgaagagggc gtggccgaca taaatgagcg cggtcgcgcc gagcttccct cccaggaccg
     6781 cccagagtgg ctggctcgac tcgtcctccc gacgctctgc aactcggaga gcgccgatgc
     6841 cgatcagcag cgtctgttgg aggatcagaa tgaagacggc agggacgacg aaggtgccgt
     6901 acccagcgag aggattgaac agtggccagt agacaaaggt ggcggggcgg gctcgccgga
     6961 gggctgcggg gctggtgagt ccagctgctt gcagccggcg taccgagatg ccggtcgaga
     7021 gcgtgccgac gacgccattg atcgcgcgaa gcgcggtgct gtacaccagg aaatagctcg
     7081 cgtcggcgta cgcttccacg taggccgcct cccggcggag tacatgacgc tcgaagtcct
     7141 tggggatcag gaggaccgcg cctgcgactc cggctcgcac cgagtcctg
//
LOCUS       DASRJQ010000434         3113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1284305, whole genome shotgun sequence.
ACCESSION   DASRJQ010000434 DASRJQ010000000
VERSION     DASRJQ010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284305"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VFB89_09840"
     CDS             <1..531
                     /locus_tag="VFB89_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27647.1"
                     /translation="LAREPAVAFVAGESGDGGLTLISPDGDAEVRRLHRHVLYRPRTG
                     DPLGLGGPRVGTDDQWLACSASDLYPDAAVQLLQLFRSPRTGDLVVVADEGFDLRGRL
                     EFPSHRAGHGSLLRAHMEIPLWSSRPLPAERLRSVDLFPAMLAWLGLDVPHPIDGRLV
                     WRPCPAAPERRFACAS"
     gene            519..1184
                     /locus_tag="VFB89_09845"
     CDS             519..1184
                     /locus_tag="VFB89_09845"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HZM27648.1"
                     /translation="MRLVTLLTRRYGGAQWDAVVRGSGAVALAAIPLVLAYPSAAGLV
                     GFVLVTIWFHGPISPFLPASYEPVLLLFGRLYPPLLVATIGTAANLYIEVVNYHLYRG
                     VLRIEALGRFRRSVEAGRLMTMFERRPFFAIWFGTVSPIPDWMGRILAAVADYPVGRY
                     LLAHGLGRFPRFLFFAAVGMYLRLPVHAVLAVTLSSILVTGAIAAARFLLRRSEPAPT
                     TSL"
     gene            1193..2095
                     /locus_tag="VFB89_09850"
     CDS             1193..2095
                     /locus_tag="VFB89_09850"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZM27649.1"
                     /translation="MWLALIAALLTDSAHVLKVPVAPGETLTVTTAGHGAPVVLVPGL
                     FGSAYGWRRVLALLTEAGYQAAVIEPLGVGTSSRPRRADYSLTAQSDRLAHAIEYLDL
                     GPAVLVAQGVGGAIALRAAARYPALVRGLVSLEGGPAERAATPGFRRAMVYVPWVKWA
                     GGVRVFRKIVRENLVKSSGDQTWITDDVIDAYTAGAAADLDGTLLAYLTMADAREPEP
                     ISQQLGAIRCPVLLVLGGAPHASRPPDEEVERLRERVRSYAEATVPGAGHYLQEERPD
                     VVAAAVLRVDRTALAQTGGGGPSQ"
     gene            complement(2675..>3113)
                     /locus_tag="VFB89_09855"
     CDS             complement(2675..>3113)
                     /locus_tag="VFB89_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27650.1"
                     /translation="VVVSTSLILAGCDQGSPTSATSPADPSFRSANTRSAVVIRNSGC
                     VLLDGDGAIASADRDIDISTQSTNQNTTLICKVKKVANSTGRALHYDSENNPIFPGLG
                     CSVLRPDGFVFTTDWNETVSAAGNATLRCRFKQLVGDSAKGQR"
BASE COUNT          485 a          995 c         1017 g          616 t
ORIGIN      
        1 ctcgcgcggg agccggcagt ggcgttcgta gccggcgaat cgggcgacgg tgggctcaca
       61 ctcatctccc ccgacggcga cgcggaagtc cggcggctgc acaggcatgt cctgtatcgg
      121 ccccgcaccg gcgatccact cggtctaggg ggtccccggg tcgggaccga cgaccaatgg
      181 ctcgcgtgca gtgcgtcgga tctgtaccca gatgccgcgg tgcagctcct tcagctcttt
      241 cgctcgccgc gaaccggtga cctcgtcgtc gttgcggacg agggtttcga cctccgtgga
      301 cgactcgagt ttccgtcaca ccgcgcgggc cacggaagcc tcctgcgtgc ccacatggag
      361 attcccctct ggtccagtcg ccctctgcca gcagagcgcc tgcgatcggt cgacctcttt
      421 cctgccatgc tcgcgtggct gggtctcgac gttccccacc cgatcgacgg ccgcctcgtc
      481 tggcgaccct gcccggcagc tcccgagcga aggttcgcat gcgcctcgtg accttgctca
      541 cgcgccgcta cggcggggcc caatgggatg cggtagtccg cggctccggt gccgtagcac
      601 tcgccgccat cccgctcgtc ctcgcgtatc catcggctgc ggggctcgtt gggttcgtgc
      661 tcgtcacgat ctggttccac ggcccaattt caccgtttct ccctgccagc tacgagccgg
      721 tgctgctgct gttcgggcgg ctctatccgc ctctcctggt cgcgacgatc ggaaccgccg
      781 ccaacctcta cattgaggtg gtcaactacc atctctatcg tggcgtgctc cgtatagaag
      841 cgctcgggcg attccgccgc agcgtggagg cagggcgtct catgactatg ttcgagcggc
      901 gcccgttctt cgcgatctgg ttcggcaccg tgtcgccgat cccggactgg atgggccgca
      961 tcctcgccgc cgtggccgac tacccggtcg gccgctacct gctggcccat gggctaggcc
     1021 gctttccccg cttcttgttc ttcgccgccg tcggcatgta cttgcggctt ccggtgcatg
     1081 ccgtcctcgc ggtcacgcta tcctcgatcc tggtaacggg ggctattgct gcagcaagat
     1141 tcttgttgcg ccggagcgag ccggcaccca cgacaagtct ttaggaggaa ccatgtggct
     1201 cgcgttgatt gctgcgctgc tcaccgatag cgcacacgtg ctcaaggtac cggtggcacc
     1261 cggggagaca ctgacggtga ccaccgctgg tcacggggcg ccggtcgtgt tggtgccggg
     1321 actcttcggc tcggcgtatg ggtggcgacg cgtgctggcc ctcctcaccg aggccggcta
     1381 tcaggcggca gtgatcgagc cgctcggagt cggcacgtcg agccggccgc ggagggcgga
     1441 ctattcgctg acggcgcaga gtgaccgcct cgctcacgcc attgagtatc tcgacctcgg
     1501 gcccgcggtt ctggtggcgc agggcgtcgg tggtgccatc gcgctccgcg cggcggcaag
     1561 gtacccagcc cttgtgcgcg gactggtttc gctggagggt ggacctgcgg agcgggcggc
     1621 gacacccggg ttccgacgcg ccatggtgta cgtgccgtgg gtaaagtggg cggggggcgt
     1681 tcgggtgttc cgcaagatcg tgcgagagaa cttggtgaag tcctcgggcg accagacgtg
     1741 gatcactgac gacgtgatcg acgcctacac agctggcgcc gccgccgacc tcgacggcac
     1801 gctcctcgcc tacctcacga tggcggacgc tcgggagccc gagccgatca gccagcagct
     1861 tggagcgatt cggtgcccgg tattactcgt gctcggtggc gcgccccatg cctcgcgtcc
     1921 gcccgacgag gaagtcgaac ggcttcggga acgggtgcgc tcatatgcgg aagcgaccgt
     1981 acctggcgcc gggcattact tacaagaaga gcggccggac gtagtggccg cagctgtgct
     2041 tcgggtggac cgaacggctt tggcgcagac tggcggggga ggcccgtccc aataacggcg
     2101 attggctaac gagttctgct gcaatcgcga gcgggtcttc tgtgtcagaa ttgtgccaga
     2161 accaggggtg gctagctgga atcagagaca gtgggccacg accctaactt ccgaaatcgt
     2221 ggccctatat gttctatgtt ggtcaaacgt gtaggggcgg atgagtactg aaaatccgcg
     2281 tatcggcggt tcaattcgtc tccactgttc ttgtcgcacg ggcgacaggc gtggggaagg
     2341 tcttgggaag gttcgggtgt aaatagccgc aagtcgctgc aattggttga gaacgggtcg
     2401 acaaccgatt ctcgaaacca caacgagtta gagctgcgca aaaccggtcg aagatggccc
     2461 tacagaactg caaaacctcc atgcggcggt tcaaatccgc ccggcgcctc tcggtgccgc
     2521 aacaacttgg ggccgctcga acgagcggcc cttcgccaat ctggggcggt tcaatctcac
     2581 tggcccagca tccagccgca gtaccccgct cctcgcctct cgtattcggc tcgctggagc
     2641 acttctcttg gaaggtaggg gcgtcctcct tccttcatcg ttgacccttc gcactgtctc
     2701 ctaccaattg cttgaaacga cagcgtagcg tcgcgttgcc ggctgcggag accgtctcgt
     2761 tccaatcggt cgtgaacaca aacccgtccg gacggagcac gctgcacccg agtccgggga
     2821 agatgggatt gttctcgctg tcgtagtgca aggcgcgtcc ggtggaattc gcgaccttct
     2881 tcaccttaca gatcagtgtg gtgttttggt tggtgctctg ggtggagatg tcaatgtcgc
     2941 gatcagcaga agcgatggcc ccgtcgccgt ctaacaggac gcagccgctg ttacggatca
     3001 caacggcgct tcgggtgttg gcgctcctga acgacgggtc ggctggcgag gtggcggacg
     3061 tcgggcttcc ctgatcacaa ccggccagga taagcgaggt gctgacaacg acg
//
LOCUS       DASRJQ010000435         3382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3078695, whole genome shotgun sequence.
ACCESSION   DASRJQ010000435 DASRJQ010000000
VERSION     DASRJQ010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3078695"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VFB89_09860"
     CDS             <1..460
                     /locus_tag="VFB89_09860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HZM27651.1"
                     /translation="GTTGLVHETYLRLVDQSRVGINDRSHFFALAARAMRHVLVDYAR
                     RRHALKRGGHEAPLPLEEGAVTIDQRADTLCALDEALTRLATFDPRLAQVVECRFFGG
                     LSEEETAQVLGVTSRTVRRDWTKAKGWLYRELTADRVERDNTWVELDRLP"
     gene            589..822
                     /locus_tag="VFB89_09865"
     CDS             589..822
                     /locus_tag="VFB89_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27652.1"
                     /translation="MKSILAATVVAVSLVACTNRAHRAEDTGAAERHSADTVVSKREM
                     QDTAIIHHDTTITSDTIHKRGAHAVKTDTVKKP"
     gene            complement(877..1299)
                     /locus_tag="VFB89_09870"
     CDS             complement(877..1299)
                     /locus_tag="VFB89_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27653.1"
                     /translation="MKPIAIPKWPLIVAALLAPAALQAQAGQVVCKDGSNGSSVAACA
                     SHGGVDSVSTAAARKARGGVETGQLDTTRGGRETGPDTALKAKPGVQTGPAAADTGAM
                     SRDTSKSKHSAKSKKHHKAADSTHMRADSAAMRPDSAH"
     gene            complement(1331..1774)
                     /locus_tag="VFB89_09875"
     CDS             complement(1331..1774)
                     /locus_tag="VFB89_09875"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HZM27654.1"
                     /translation="MARINEAITDRNGQVVVPAESPVQLSVAQLEPAKTRSATDGKLA
                     LRVDSVLVEGRAYPVAAKVEPVPHELRGRGVSAGEAEKVGVGAAAGAVLGRVLGGNTK
                     GTIIGGAVGAAGGAVVAAQTASRDVVVKPGTWVSFVLTAPLVVKE"
     gene            complement(3111..>3382)
                     /locus_tag="VFB89_09880"
     CDS             complement(3111..>3382)
                     /locus_tag="VFB89_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27655.1"
                     /translation="VAGLENQEGQPEVEPINFLPHIKIPVLMLNGQYDHYFPVETAQR
                     PMFRLLGTPPDKKRQVISEGGHFVPRVQLVKETLDWLDRYLGPVQ"
BASE COUNT          540 a         1090 c         1127 g          625 t
ORIGIN      
        1 cggcacaacc ggactggttc acgagacgta cctccgtctg gtagatcaga gtcgagtcgg
       61 catcaacgac cggagccatt tttttgcgct ggcggcccgc gcgatgcggc acgtgctggt
      121 ggactacgcc aggcggcgcc atgcgctcaa gcgcggggga cacgaagcac ctcttccctt
      181 ggaagagggc gcggtgacga tcgaccaaag ggccgacact ctgtgcgcgc tcgacgaggc
      241 gctcactcgg ctggccacgt tcgatccgcg gttggctcag gtggtggagt gccgcttctt
      301 cggcggcctg tcggaggaag agactgctca agtgctggga gtaacctctc gcacggttcg
      361 acgtgattgg accaaggcaa aggggtggct ctaccgcgag ctgacggctg accgagttga
      421 gcgcgacaat acctgggtag aactggaccg gctcccctga gaggcacgcc cttctagtcg
      481 tgaggagcct cacggcgtgg cgcgctcggc aacttgcatg tattcgctgc agagctggag
      541 tcggctcgtg ggagccggca gtctgcgtta cctgaatccg aggtcccgat gaaatcgatc
      601 ctggcagcca cggtcgttgc cgtaagcctg gttgcatgta ccaaccgcgc gcaccgcgca
      661 gaggacaccg gcgcagccga gcgacattcc gccgacaccg tcgtttcgaa gcgcgagatg
      721 caggacacgg ccatcattca ccacgacacg acgatcacga gcgatacgat tcacaagcgt
      781 ggcgctcacg cggtgaaaac ggatacggtg aagaagccgt agcaagcccc gggcgattgc
      841 ccggggcagt tgctttggcg caggcaggct ccccgcttag tgcgcgctat ccggccgcat
      901 ggcagccgag tcagcccgca tgtgagtgct gtcggcggcc ttgtgatgct tcttcgactt
      961 ggcgctgtgc tttgattttg acgtatcgcg gctcatggcg ccggtgtcgg ccgcggctgg
     1021 gccagtttgc acgccgggct tggccttgag cgccgtatcc ggtccggttt ccctgccgcc
     1081 cctggtagtg tcgagctgcc cggtttccac accaccgcgc gctttccggg cagccgccgt
     1141 ggacacgctg tccaccccgc catgactggc gcaggcagcc acgctcgagc cgttcgatcc
     1201 atccttacaa accacctggc ctgcctgggc ctgcaacgcc gccggcgcaa gcagcgccgc
     1261 gacgatcaga ggccacttgg gaattgctat gggcttcatt ttttcctcgc gattgagttg
     1321 agatgagctc ctactccttc accaccaaag gcgcggtgag tacaaacgag acccaggtcc
     1381 ccggcttgac gaccacgtcg cgactggctg tttgagccgc cacgaccgca ccgccggcgg
     1441 cgccgacggc acctccgatg atcgtaccct tggtgtttcc accaaggacc ctgcctaaaa
     1501 ccgcgcccgc cgcagcacct actccgacct tctcggcttc accggccgag acgccgcggc
     1561 cacgcagctc gtgcggcacg ggttcaactt tcgcggcgac ggggtaggcc cgcccctcga
     1621 ccaacaccga atccactcgc agggcgagtt tcccatcggt cgcgcttcgc gtcttggcgg
     1681 gctcgagctg cgcaaccgag agctggacgg gagattccgc aggcacgacg acctgtccat
     1741 tccgatcagt tatggcttca ttgattcgcg ccatcacgat cttccccgcg gaatcgtggc
     1801 gcgaattcag tgagtcctgc agctgggctt ggaccgtagt tcccgccgga aggctgcgcg
     1861 ctgccggagc aaaagggtcc gatcgattcg tcggtgacac cgtcccgggg atgggggacg
     1921 gagccgagtc tcgcacagtc ggtggcgagg gctccgtcgc cacctctgtg tcaggagccc
     1981 cagccgcggg ctgcttcgag accgtgtctg ggtccgggct gggggaggga atcggcgtgg
     2041 aggctggtgc gggcgacgtc ttttcggtcg tgctgtcagg aggagcgctt ctgccgggcg
     2101 atgacgcatg gggtcgcgat ttaagctgca gggggcgttc gtcgggcccg gcgtgaggcg
     2161 gactgcttgc gcgacgagag ctcgggccgc tatccggcaa cgcctcttcc aggggccggg
     2221 cgacccggtc gacgctcgcc gtctcctgag ttcgtcgctc cgagcacgcg gataggattg
     2281 tgagaccgac caaaatgaac gccaggttcc tcattgagcc aaccgggttg gatgccttta
     2341 gtctccgccc cgtgcccatg ctacgccgtg gcttggctca ctttttcaca gatgggcctg
     2401 ctttcctgca gtgggttgca gtgccgcggg ccgcggcggc cccgtgatgt tgcgagggtt
     2461 cgaagctccg gtttagcccc gcgcaaacct gaaacatcag cgctaatcgc tcatgcgagg
     2521 cttgtttgca ccagcgggct ccagggcggt acgacaaatg ccgcgacgca cgtatcccag
     2581 cgcgacaacc tgactcatgc cgaaatccgc accgggcgct cagcccgatc cgatggcgcg
     2641 ggtggtggag cgtctcctcg cccagctgga gaattcaggg cccaggcctg cccaggagcg
     2701 ggtgacttca cctgcgagtt cgccgagcca tcagaacggg acgacgcgga aaccgcggcc
     2761 ggtggtgctc tcgggcaagg ccccaagccg cgcacccctc atcgcccttt gggtcggcct
     2821 tatactgggc atcgttctcg gcggggcgat gacacaatgg ccgtattcgc ggggatgcga
     2881 cttgcccctc ttcgggtatc tcgctggggt cgctgcagtc atcgccgccg ggggctcgag
     2941 cgcagtggcc gcctggagac tgcgaagcgg ggccgcgcac gtgctcgcgc tgctgttgct
     3001 cttctggggt atcgcgctgg cggcggagcg ggtgttgccg cgggttggct atgcggcgga
     3061 gcgggccagc tggcggtgct cggcggaacc gccggcttcg ttggcgggat ctattgtacc
     3121 ggtccgaggt agcggtcgag ccagtccaac gtctccttga ccagctgcac ccgtggcacg
     3181 aagtggccac cctctgagat gacctgccgc ttcttgtcgg gcggcgtgcc gagcagccgg
     3241 aacatgggac gctgggcggt ctccaccggg aagtagtggt cgtactgccc gttcagcatc
     3301 agaaccggga tcttgatatg cggcaggaaa ttgatcggct ccacctccgg ctggccctcc
     3361 tggttctcta gcccggcgac ca
//
LOCUS       DASRJQ010000436         2833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_333735, whole genome shotgun sequence.
ACCESSION   DASRJQ010000436 DASRJQ010000000
VERSION     DASRJQ010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333735"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VFB89_09885"
     CDS             complement(<1..202)
                     /locus_tag="VFB89_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27656.1"
                     /translation="MVARSDAEAASLWPGTRTRAMLPLAGKATPSLDNGKIPVDLRAQ
                     LQVALADRYRVEQELGRGGMATV"
     gene            210..863
                     /locus_tag="VFB89_09890"
     CDS             210..863
                     /locus_tag="VFB89_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27657.1"
                     /translation="MPPRPTPYDLVFAPLAEQRFPAIRAALSDTGSDPRDRDAFLLTA
                     EAARLIHDLRPDEGLGEDIDRLAALVHHAFLFWEHGSELVSLDRTLTDKLLGSPPPSA
                     EGTPAQAPYVQVPERQIWARPVEQEAYEPLDGWFAHVAPGSLLRVLAVFGLHPDRMGF
                     TVVEAIGPRSTNLLRPDGSPLFAPTLRGGDAARLREILGGEELVELGWRVRDAGKTP"
     gene            863..2596
                     /gene="polX"
                     /locus_tag="VFB89_09895"
     CDS             863..2596
                     /gene="polX"
                     /locus_tag="VFB89_09895"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HZM27658.1"
                     /translation="METMDKKAVAQVLEQIASFMELKGDNPFRIRAFRTAARALAGIP
                     GELGEALQDGSLASTRGIGPAILQIIGELASTGRSSMLEELREQVPPGLVEMLSIAGL
                     GVAKIRQIHDLLDIDSLPELEAAALDGRLAKLPRFGAKTSENILRGIAYLRQATIYRL
                     SHHAAEEAAALKEALEKLPGVLRAIIAGDVRRRAELVKDVVLVVEAEVPPAELFERLG
                     RLPGVHEFAGQDERRVTLRFAGGASAQIIVTTPVNLGAVLVQATGSETHLEALAKHAA
                     AKGYDLSGAALWRGHEFVPTPSEGELYRALALAEIPPELREGRGEVEAAAAGKIPVLV
                     ERAQLRGLLHCHTNYSDGSNTVEEIALGCRSVGYSYVGLTDHSQAAAYAGGLAAEDLA
                     RQAEEVDAANKRLDGIRVLKGIEADILVDGSIDYDAAVLARLDFVIASIHSRFNLNAA
                     EMTTRMLAAMDNPYVTIIGHPTGRLLLSREPYDVDLDALFEKAASRGVAMEINADPHR
                     LDLDWRVLRRAREAGVTISIGADAHNVSSIGNVEYGVGMARKGWLGPNDILNARPLEE
                     FLEFVAQRRTG"
     gene            2593..>2833
                     /locus_tag="VFB89_09900"
     CDS             2593..>2833
                     /locus_tag="VFB89_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27659.1"
                     /translation="MKRRGTSLARAVVRAPIIFERLHAAYPEAKTALSHTNPFELLVA
                     TILSAQTTDVRVNLVTPALFARYPDAAALARARQSD"
BASE COUNT          478 a          924 c          942 g          489 t
ORIGIN      
        1 agacggtcgc catcccgccg cgaccaagct cttgctcgac gcggtagcga tcggccaacg
       61 cgacttggag ttgggccctg agatccaccg ggattttccc gttatcgaga gaaggggtgg
      121 ccttgccggc caggggaagc atagcccggg tgcgagttcc gggccagagc gaggcggcct
      181 cggcgtcaga ccgagctacc attctccccg tgccaccacg cccgaccccc tacgacctcg
      241 tcttcgcccc attggcggaa cagcggtttc ccgccattcg agccgctctt tccgacaccg
      301 gcagtgaccc gcgggatcgc gatgctttcc tgctgaccgc tgaagctgcc cggctgatcc
      361 atgacctccg ccccgacgag ggcctgggcg aggacatcga ccggctcgcc gcgctggtgc
      421 atcacgcctt cctgttctgg gagcacggga gcgagctggt ctccctcgac cggacactaa
      481 cggacaagct gctgggatcg ccgccgccgt ctgccgaagg cacgcccgcc caggcgccct
      541 acgttcaagt ccctgagcgg cagatctggg ctcgcccggt ggagcaggag gcctacgaac
      601 cactcgacgg ctggtttgcc catgtggctc cgggcagcct ccttcgcgtg cttgccgtct
      661 tcggcttgca cccagacagg atgggtttca ccgttgtgga ggcgatcgga ccccgttcga
      721 ccaacctgct tcggccggac ggctctccgc tctttgcacc cacccttcgc ggtggcgatg
      781 cagcccgcct gcgcgagatc ctcggcggtg aggagctagt cgagctgggt tggcgggtac
      841 gcgacgccgg aaagacgcct taatggagac catggataag aaggcggttg ctcaggtcct
      901 ggagcagatc gcctctttca tggagctcaa gggagacaat cccttccgaa ttcgagcgtt
      961 ccggacggct gcccgggcgc ttgccggcat tccgggtgag cttggcgagg ccctccaaga
     1021 cgggtctctg gccagcaccc gcggtatcgg accagccatc ctccagatca tcggtgagct
     1081 tgcctccacc gggcgctcgt ccatgctcga ggagctgcgt gaacaggttc cgcccggcct
     1141 ggtggagatg ttgtccatcg cgggccttgg cgtggccaag attcgccaga ttcacgatct
     1201 gctggacatc gactcactcc ccgagctgga ggcggcggcg ttggacgggc ggctggcgaa
     1261 gctgccgcgc ttcggtgcca agacctcgga aaacatcctc cggggaatcg cctacctccg
     1321 ccaggcgacg atttatcgcc tctcgcatca cgccgccgag gaggctgcgg cactgaaaga
     1381 ggcgctggaa aagctccctg gcgtcctgcg cgcgatcatc gccggcgacg tgcggcgacg
     1441 agccgagcta gtgaaagacg tcgtactcgt ggtcgaggca gaggtgccgc ccgccgagct
     1501 gttcgagcga ctgggaaggc tgcccggcgt acatgagttt gccggtcagg acgagcgccg
     1561 ggttactctg cgattcgccg gcggcgccag cgcgcaaatc atcgtcacca cgccggtcaa
     1621 ccttggcgcg gtgctggtcc aggcgaccgg gagcgagaca catctcgagg cgttggcaaa
     1681 gcacgccgcc gcgaagggct acgatctgag cggtgcggct ctgtggcggg gccacgagtt
     1741 cgtgccgacg ccatccgaag gtgagctgta ccgtgctctc gcattggctg aaattccgcc
     1801 ggagttgcgg gaggggcggg gcgaggtcga ggccgccgca gccggcaaga tccctgtgct
     1861 ggtcgagcgc gctcagcttc gcggactgct ccactgccat accaactatt ccgacggatc
     1921 caacacggtc gaggagatcg ccctgggctg tcgatccgtc ggctactcgt acgtcggttt
     1981 gaccgatcat agtcaggccg cggcctacgc gggcggactt gccgcggagg accttgcgcg
     2041 tcaggcagag gaagtggacg cggcaaacaa gaggctggac gggatccggg tactcaaagg
     2101 catcgaagcc gacatcctgg ttgacggcag catcgattac gacgccgccg tactcgctcg
     2161 gctcgatttc gtcatcgctt cgatccacag ccgattcaat ctcaatgccg cggagatgac
     2221 cacacggatg ctcgccgcga tggacaaccc gtacgtgacc atcatcgggc acccgacggg
     2281 ccggctgctg ctttcgcgtg agccgtacga tgtcgatctg gatgccctgt tcgagaaagc
     2341 cgcctcacgg ggggtcgcaa tggagatcaa tgccgatccc caccggctcg atctcgactg
     2401 gcgggtcttg cggcgagcgc gcgaggccgg cgtcacgatc tccattggtg ccgacgctca
     2461 caacgtgtcg agcatcggca acgtggagta cggtgtggga atggcgcgga agggctggct
     2521 gggtccgaac gacattctca atgcccggcc gctggaagag ttcctcgagt tcgttgcgca
     2581 gcggaggacg gggtgaaacg gcgggggacc agcctggcca gggcggtggt ccgcgcgccg
     2641 atcatcttcg agcggctgca tgcagcctat cctgaagcga aaaccgcgct gagccacacc
     2701 aatcccttcg agctgctcgt agccacgatc ctgtcggccc agaccacgga cgtcagggtg
     2761 aacctggtga cgccggcgtt gttcgctcgc tatcctgacg cggcggcact ggcccgcgct
     2821 cgacagagcg acg
//
LOCUS       DASRJQ010000437         9492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_217953, whole genome shotgun sequence.
ACCESSION   DASRJQ010000437 DASRJQ010000000
VERSION     DASRJQ010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 9492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9492
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217953"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="VFB89_09905"
     CDS             <1..965
                     /locus_tag="VFB89_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27660.1"
                     /translation="PVLCGLVVGAIAAALVGHALARGPFASDFDQLWIAGRALLEGSD
                     PYTAVASAHINLGYPLYYPLPAVILTLPVALLPLAAARLAFAVACGFIAGFGLQRLGL
                     YALLVVLSPLFQTPIIQGQIAPALAGAALVPMLGFLLVAKPTIGLALWISRPSRRAAI
                     GAFAFVALTVVLWPWWPRAWIDAIRVAPHILPPIMRPGGVVLLLGALRWRRPEGRLLA
                     AWAFVPHTEALYDLTPLILVAASAADALALVACSWVALVAHFMLRQPGVDLSVSIAAQ
                     WPVTLALLYIPALILVLARPNRAPKEAPAQVVQPLPAKYASSSP"
     gene            1024..2007
                     /locus_tag="VFB89_09910"
     CDS             1024..2007
                     /locus_tag="VFB89_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27661.1"
                     /translation="MIGVGVGIAAALLAWVAHGFPMEGHSDFDQLWIAARALQTGADP
                     YEAVRRVEAYPLFYPLPAVLLASPMAFFPLELAKVIWGGLGAGLLAYAAFTYKRALPT
                     ALLGAGFLNAIVVAQWAPFLTAAAVLPWASVAWVAKPSIGLALLIAYPHRKALFYGGA
                     ILVVSLIAMPSWPGEWLHALRDSIQMPLALRPGGFVLLLAALRWRTPEGRLLTALACV
                     PQTVGLYETLPLFLIPRTRWEGYALATLSFVAAAIQETLAGHTITEFINSKWPSLLLF
                     CYLPALVILLRRPNRPSRSGDLAEPLSEHHRVEGAVGKVDRHTPAEPGAGV"
     gene            2000..2974
                     /locus_tag="VFB89_09915"
     CDS             2000..2974
                     /locus_tag="VFB89_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27662.1"
                     /translation="MSETAPSRNALWLALAAAGLGGLICWLSYRLPPPSVSDFDNLHI
                     ATRGLLSGKDPYAAVVASGIGFPLFYPLPACLLVLPVAWLPLPLARAGWATLSAGVFT
                     LAALRYRRGLPVALLSAPFLNAIVLGQWSPLLTAAVAFPLLGCVWAAKPSLGAALFVS
                     FPSRRALGSALAIVVLSLIILPGWPQGWLGALQASNHKLPILQPGGFLLGLALLRWRT
                     REARLLLALACTPQTIGLYELLPLFLIPRRRREAYLLAILSYAAAFISVLLFPWPPGA
                     AIEIVRAHRWPVLLALVYLPTLMLVLLERPATDVPNASSSPSPEAHDQ"
     gene            complement(2864..4636)
                     /locus_tag="VFB89_09920"
     CDS             complement(2864..4636)
                     /locus_tag="VFB89_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27663.1"
                     /translation="MVWALALLLVGLTVAAQLTALATPDIGFLLYAAGRMLDGAHLYR
                     DIVEINPPLILWLNLPVVWVARRLHVSDLHLYVLATAIAVAALLLFSRRLARRYALDD
                     APALRRYVLLLLCIGFFPFAGVDYGEREQFVLALILPYVFLTVAQVRRRRLALGDATV
                     VGALTGVGLALKPHFALVWFLLELFRRWRMPGERWRIRPEVGAMIGVLGFYMLLIVLV
                     TPEYLFLAGFLGPTYAKFLRDPFLHLLFLAPGAALVLFALLAYLALRNRLEWPVLTAT
                     FCVATAACYLAGAAQEKGFPYHFYPALVLGFVLLGLMAWVEPLPGWRVSERLYAFFLK
                     PVTLTVALIALGATLVTLTGGGPEARRQRRECLDDAAVVRAHAGGRPVGILSFHLGSA
                     FPLLNYAGVPLAMRFPHLWILPASYWDSLTAGGALRYREVEEMPPVERWMYAAVKEDL
                     LAAQPKVLMVLRPARDSATNGLRRLHYIRYFERQPELAAFFARYELLEHRGEFDMYQR
                     LEDEARRTAPPPSDLPGTQDVKHADLGKVNLALGDPKFASGVAVFALLVVGFWARRRR
                     GVRDVGRRPFQQDQHQRRQVDQRQ"
     gene            complement(4699..5490)
                     /locus_tag="VFB89_09925"
     CDS             complement(4699..5490)
                     /locus_tag="VFB89_09925"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HZM27664.1"
                     /translation="MREQPAFSIVIPAYNEEARLEATVRAYLGYFRTRGEPVELLVVD
                     DGSLDGTTYLVDRLSIEFEQLRLIRLAQNQGKGYAVRSGIVNARGRLILFADADGATP
                     IQEIERLEAAIAKGADLAIGSRALASEQVQVRTQIHRRIIGRGFHAMVRLCGVRGIAD
                     TQCGFKLFRGPVAHFLFSRMRTSGFSFDVEVLLMARVFGFVVAEVPVNWVHQPGSRVN
                     LVTDSAQMAWDLLWIRLHHLRGEYRTPRLAPWALSEAKHPAGAHL"
     gene            5652..7319
                     /locus_tag="VFB89_09930"
     CDS             5652..7319
                     /locus_tag="VFB89_09930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZM27665.1"
                     /translation="MRAHAAAVVAAGLLLGACLRIAATYSVFNQTWDEPAHIATGLEW
                     LQQGRYDFEPLHPPAARVAVALGPYLGGARLTPDDANPWTQTARILGTGAAYFRMLAL
                     ARVANLAFFLVAGVALFCYVLRALGRWPAAVSILLLSTVPPFLGHAGLATTDVALAAG
                     VMVSLTVLILWLDQPSRLRGVLLGAAIGGTVLTKFSALLLLPLIFAVTIATCRLCDKL
                     ESPTRKAGVPLHRLLRPVWLTALVVLWTGYRFSVGPIVPVRGPQHCESSTPCLADSLP
                     PAVAALATAPVYPAPEFVRGLVAYAREGRTGRKAYLLGRRTFGGWWYFFPIAIAIKTP
                     LPFLVLTVVGTAALVVARNRSQAWLVLAAGASAGAILLALIPSRVNIGLRHALAVYPL
                     LSAVAAYGVSRLWQLKPHLPARSLLVGLLAWQVVDSVRAAPDFVAYFNELAPGPPEQY
                     LVDSDLDWGQDLHRLADTLRARGIGQVALAYNGSADPGAVGIEGYRWLRPFTRDTGWI
                     AVSVFALEVGIWNQPTWDDYAWLRSRQPVARAGRSILLYRVVPDTIQ"
     gene            7316..8254
                     /locus_tag="VFB89_09935"
     CDS             7316..8254
                     /locus_tag="VFB89_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27666.1"
                     /translation="MRETARRIALPLGVGCVAAAIVWASFRTPGVASDFDHLWIAARA
                     LTRGDDPYRAVLEAGNRYPLFYPLPAVLLVLPLAGLPVGLARVIWSGIGAAVLAEAAR
                     RYGRGLPPALLSAGFAAAIVQGQWSPLLTASVVLPVLAVAWVAKPSIGLAVAAAAPSW
                     RVVGWALGLLVVSLALRPTWPRGWIDAVQSQIYVAPILRPGGIMLLLALLRWRRPEGR
                     LLAVLACVPHSPTAYETLPLFLIPRTRWEGYGLAALGYAAAFLGAWLTPWSHAIETLP
                     QDLSRQWPFMLLLVYLPALVLVLRLPNTPGSVGATS"
     gene            complement(8307..>9492)
                     /locus_tag="VFB89_09940"
     CDS             complement(8307..>9492)
                     /locus_tag="VFB89_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27667.1"
                     /translation="FVLAGIAAASYPTFRRKPGVFGAVVLCWGLSFAVAYWLVYRSAS
                     ANPYLRWFWSERFLTLWVPGVLGRAYGAAREVLFTSVVSDVSEVADTLLGNGCLLLTA
                     GLVGFLVLLGVRRLMRTNPSIAILFIGSISASLLASCLGLYPVAARMMLFAVPILLTL
                     LVVGATDLYRRRRRLRMGIAGVLGVIVLAGQLRNVVRADDPYRSGHLRPAVAFLQTAL
                     LPGEPIYVEAGVLPAWTFYTTNWSHPDISRLERMAREASFGGGAFENQRSRGRRVSGD
                     GAELVFPFHGGVELLGVGDGGAFRVGARHKPPSDPGWAASEAQRIRMAARPTAWLVTI
                     KLPWADIKLDEAIRSAGGVRRDSLVADYAVVARYQFANESASTPVNLDQSPEPRPGND
                     SR"
BASE COUNT         1601 a         3153 c         2958 g         1780 t
ORIGIN      
        1 gaccggttct gtgcggtctc gtcgtggggg cgatcgcagc cgcgctcgtg ggtcacgcgc
       61 tggctcgcgg cccgtttgcc agcgatttcg atcagctctg gatagcgggg cgcgccctgc
      121 ttgaaggctc ggacccctac acggcggtcg cgagcgcaca catcaatctc ggctaccccc
      181 tctactaccc gctccctgcc gtcatactga ccctgccagt ggcgctcctg cccctcgcag
      241 cagctcgcct ggcatttgcc gtcgcgtgcg ggttcatcgc gggcttcggt ttgcagcggc
      301 tcgggcttta cgcactcctc gtggtgctga gccctctgtt ccagaccccg atcattcagg
      361 gtcagatcgc gccggcgctc gccggtgcgg cactggtgcc gatgctggga ttcctgttag
      421 tcgcaaaacc cactatcggt ctcgcgctct ggatttcccg cccgtcccgt cgggcggcga
      481 ttggcgcctt cgcgttcgta gcgctcacgg ttgtgctctg gccctggtgg ccccgcgcgt
      541 ggatcgacgc tattcgcgtc gcccctcaca ttctcccgcc catcatgcgt ccggggggag
      601 tcgtgctgct gctgggagcc ctgagatggc gtcggccgga agggcggctg ctcgccgctt
      661 gggcgttcgt gccgcatacc gaggcgctct acgatctcac cccactgatt ctggttgcgg
      721 cctcagcggc agacgcgttg gcgctggtgg cttgcagttg ggtggcactg gttgcgcatt
      781 tcatgctgcg gcagcctggc gtcgatctgt ccgtcagcat cgcggcccag tggccggtga
      841 cgcttgccct cctctacatc ccggctctga tcctggtgct cgcccgccca aaccgcgcgc
      901 ctaaggaagc tccagctcaa gtcgttcaac ccttgccggc gaagtacgca tcctcgagtc
      961 cgtgatgggg cacggatcgc agcgcgaagc ctgagatagt ccatgtctcg atttggccgg
     1021 ataatgatag gcgtcggcgt cggtatcgcg gccgctctgc ttgcgtgggt ggcccatgga
     1081 tttccgatgg aaggccactc cgatttcgac cagctctgga tcgcggcgcg agccctccag
     1141 acgggagctg atccctatga ggcggtgcgc cgagtggagg cgtacccctt gttctatccc
     1201 ttgcccgcgg ttctgctggc ctcaccgatg gcctttttcc ctctcgagct cgcaaaggtc
     1261 atctggggag gcctcggagc cggcctgctt gcctacgccg ccttcaccta taaacgagcg
     1321 ctcccgacgg cgctgctcgg cgccggcttc cttaacgcga tcgtcgtggc gcaatgggcc
     1381 ccattcctga cggccgcggc cgtgctgccc tgggcgtcgg tggcttgggt cgccaagcct
     1441 tcgatcggcc tcgcgctctt gatcgcctat ccgcaccgca aggcgctctt ctacggcggc
     1501 gcgatcctgg tagtgagtct catcgcaatg ccatcctggc cgggcgaatg gctccacgcc
     1561 ctgagagatt cgattcagat gccacttgct ctcaggccgg gcggcttcgt ccttctcctc
     1621 gcggcgctcc gctggcggac gccggagggc cggttgctca cagcgttggc ctgcgtgccg
     1681 caaaccgtcg gcctctacga aaccctgccc ctttttctga ttccacggac tcgctgggag
     1741 gggtatgcct tggccaccct ctcgttcgtc gccgccgcga ttcaggagac tctagccggg
     1801 cacacgatca ccgaattcat taactcgaaa tggcccagcc tgctcctctt ttgctaccta
     1861 ccggccctcg tcatactgct tcggcggccg aaccgtccgt cccgctcagg cgaccttgcg
     1921 gagccattga gcgagcatca tcgcgtcgaa ggagccgttg gcaaggtgga tcgccacaca
     1981 ccagccgagc cgggcgcagg tgtctgaaac tgcaccctcg cggaacgcgc tctggctcgc
     2041 gctcgcggcg gcgggactcg gcggcctgat ctgctggctg agctaccggc tgccgccgcc
     2101 atcggtttct gatttcgaca accttcacat cgctacccga gggttgctct ccggcaagga
     2161 tccctatgca gctgtcgtgg catcggggat cgggtttccg ctattctatc ccctcccggc
     2221 ttgtctgctc gtgcttcccg tcgcctggct cccgttgccg ctcgcccgag ctggatgggc
     2281 cacgttaagc gccggcgtgt ttaccctagc ggcactgcga taccgccgcg ggcttccggt
     2341 agcactgctg agtgctccat tcctcaacgc gatcgtgctg ggtcagtggt cgcccctcct
     2401 gactgccgcc gtggcgtttc ctctgcttgg atgcgtctgg gctgccaagc catcacttgg
     2461 agccgcgctt tttgtctcgt tcccttcacg ccgggccttg ggaagcgcgc tcgcaatcgt
     2521 ggtgctgagc ctcatcatcc tgccgggctg gccccagggt tggctcggtg ccttgcaggc
     2581 ttctaatcac aaactgccca tcttacagcc ggggggattt ctgcttgggc tggctttgtt
     2641 gcggtggcgg acccgggaag cgcgcctgct cctggccctg gcgtgcaccc ctcaaaccat
     2701 agggctttac gagttgttgc cgctgtttct gattccgcgc cggcgacgag aggcctattt
     2761 actcgcaatc ctgagctacg ccgctgcgtt catctcggtc cttctgtttc cgtggcctcc
     2821 gggtgccgct atcgaaatag tacgggccca ccggtggccg gtgttactgg cgttggtcta
     2881 cctgccgacg ttgatgctgg tcctgctgga acggccggcg accgacgtcc cgaacgcctc
     2941 gtcgtcgccg agcccagaag cccacgacca gtagcgcgaa gactgctaca ccagacgcaa
     3001 atttggggtc ccccagggcg agattgacct tgcccaggtc ggcgtgcttc acgtcctggg
     3061 tgccgggaag atccgaggga ggcggtgccg ttcgcctggc ttcgtcttcg agccgctggt
     3121 acatgtcaaa ctcacctcgg tgctccaaca gttcgtaacg ggcaaagaac gccgcgagct
     3181 cgggctgtcg ctcgaaatag cggatgtaat ggagtcgccg gagcccgttt gttgccgagt
     3241 cgcgggccgg acgtaacacc atcaggacct tgggctgggc ggccaagaga tcctctttca
     3301 ccgcagcgta catccaccgc tcaaccggcg gcatctcttc gacctcgcga taccgcagcg
     3361 caccgccggc agtcagcgag tcccagtagc tggccggcag aatccataag tgcggaaagc
     3421 gcatcgcaag cggcacgccg gcataattca ggagcggaaa cgcagaccct aagtggaacg
     3481 aaaggatccc caccgggcgg ccaccggcat gagcccggac cactgcggcg tcgtccagac
     3541 actcgcgccg ttgacgccga gcttccggac caccgccggt caaggtcacc agggtggcgc
     3601 caagagcgat cagggccacg gtaagcgtga ccggcttcag gaagaatgcg taaaggcgtt
     3661 ccgagacccg ccagcctggc aaaggctcaa cccatgccat gagcccaagg aggacgaacc
     3721 caagcaccag cgcaggatag aagtgatacg ggaacccttt ctcctgggcc gccccggcga
     3781 gatagcacgc tgctgtcgca acgcagaacg tcgcggtcag gaccggccac tcgagtcggt
     3841 ttcggagcgc gaggtaggcc aatagcgcga agagcaccag cgcggcgccg ggggccagaa
     3901 acagaagatg aagaaaggga tcacgcagaa acttggcgta ggtcgggccg agaaaacctg
     3961 cgaggaacag gtactccggc gtcaccagaa cgatcaacag catgtagaac ccaagtacgc
     4021 cgatcatggc gccgacttcc ggccggattc tccagcgctc ccccggcatc cgccagcggc
     4081 gaaacagctc gagcaggaac cacaccagcg cgaagtgcgg cttgagcgcg agtcccacgc
     4141 cggtaagggc accgaccact gtcgcatcgc ccagcgccag acggcggcgg cgtacctggg
     4201 ccactgtgag aaacacgtag ggcaggatta acgcgaggac gaactgttct cgctctccgt
     4261 agtcgacccc ggcgaacggg aaaaacccaa tgcatagcaa caggagcaca taccgcctga
     4321 gcgcgggagc atcgtccagg gcgtagcgac gagccagacg gcggctaaaa aggaggaggg
     4381 ccgccactgc gatggccgtg gccaggacgt agagatggag gtccgagacg tgcagccgcc
     4441 gggcaaccca gacgacagga aggttgagcc agagaatgag cggtgggttg atttctacga
     4501 tgtcgcggta gaggtgcgca ccgtccagca tgcgtccggc ggcgtagagc agaaatccga
     4561 tatcaggagt agcgagcgcc gtgagttgtg cggctactgt cagacccaca agcagcagcg
     4621 cgagcgccca caccaagcca cggcgttccc acaacccgaa ggcccacccc ggagcggacg
     4681 ggcgctcggg ctcgtgtgtc agagatgcgc tccggcggga tgcttggctt cggacagcgc
     4741 ccatggggcg agtcgcgggg tccggtattc acctcggaga tggtgcagac gaatccaaag
     4801 gagatcccag gccatctgcg cagagtcggt caccaggttg acccgtgagc caggctgatg
     4861 gacccagttg acaggaacct ccgcgacgac aaagccgaat acgcgggcca tcagaaggac
     4921 ttccacatcg aagctgaacc ccgatgttct catgcgggaa aacaagaaat gggcgaccgg
     4981 gccacggaag agcttgaacc cacactgcgt gtcggctatc ccccgcacac cacacagccg
     5041 caccatcgca tgaaacccgc ggccgatgat gcggcggtgg atttgagtcc gcacctgtac
     5101 ctgttcggat gcgagagctc gcgagccgat ggcaaggtcg gcgcctttgg cgatggcagc
     5161 ctcgagcctt tcgatctcct ggatcggcgt ggcaccatcg gcgtccgcaa acagaatcaa
     5221 ccggccacgc gcgttaacaa tgcccgaccg gacagcgtat cccttgcctt gattctgggc
     5281 cagcctgatc aagcgcaact gctcgaactc gatgctcagt cgatcgacca gatacgtggt
     5341 tccatccaag ctgccatcat caacgaccag caattcgacc ggctcacctc gagtccgaaa
     5401 gtagccaaga taggcccgta cggtcgcttc gagcctcgcc tcctcgttgt aggcaggaat
     5461 tacgatcgag aacgctggtt gttcgcgcat tgtggggtcc atcgagcaac tggcatacca
     5521 gtggcagggt cgcataagtt tgtgcccacc cgcaaccttg cgccatccca ccgctccgac
     5581 atgcgggcga ctgtcgtggg ttcacgccac gtgtagcggg tcacctacag ccgacgaacg
     5641 gattctctac cttgcgcgcc cacgcggcgg ccgtggttgc tgcggggctc cttctcggag
     5701 cctgtctccg gattgcggca acctactcgg tcttcaacca gacctgggat gaaccggccc
     5761 atatcgccac cggcctcgag tggcttcagc aaggccgcta cgacttcgag ccgctgcacc
     5821 caccggcggc gcgggttgcc gtcgcgcttg ggccgtatct cggcggcgcg cggttgactc
     5881 cggacgatgc gaatccttgg actcagaccg cccgcattct cggcaccggc gcagcttact
     5941 tcagaatgct ggcgctggcg cgagttgcca atctcgcgtt ctttctggtt gccggtgttg
     6001 cactgttctg ctacgtcctc cgagcgttgg gacgatggcc tgccgcagtt tcgattttgc
     6061 tccttagcac agtcccgccc tttctgggcc atgccggcct tgcgaccacc gacgtcgcgc
     6121 tcgcggccgg cgtgatggtc agcctgacgg tccttatcct ttggctggac cagcccagtc
     6181 ggctccgggg ggtgttattg ggtgcggcga tcggtgggac agtgctcacg aagttctcgg
     6241 ccctgctcct tctcccgttg atcttcgcag tcacaatcgc gacttgccgg ctttgcgaca
     6301 aacttgaatc gccgacccga aaggcaggcg tcccattgca ccgcctgctt cgcccggtgt
     6361 ggctcaccgc gctggtcgtg ctgtggaccg gctatcgctt ctccgtcggc ccgatcgttc
     6421 ctgtccgagg gccgcagcat tgcgaaagct caaccccgtg cctggcagac tctctcccgc
     6481 cggcagtcgc cgcgctggcg actgcgccgg tgtacccggc gccagaattc gttcggggcc
     6541 tggttgccta cgctcgtgaa ggccggacag gacgcaaagc gtatctgctg ggccgcagga
     6601 ccttcggcgg ttggtggtac ttctttccga ttgcaatcgc gatcaagacc ccgttgccgt
     6661 ttctcgtact gaccgtcgtc gggacagccg ctttggtggt cgcacgtaat cgcagccaag
     6721 cctggctcgt gctggctgcc ggtgcctctg ccggcgcaat cctgctggcg ctgataccca
     6781 gccgggtcaa cattggactg cggcacgcat tggcggtcta tccattgttg tcggccgtcg
     6841 cagcgtacgg ggtgagtcgc ctctggcaac tcaagccgca cttgccggcc cgttccctcc
     6901 tggtcggcct tctggcctgg caggtcgtag actcggttcg cgccgcaccg gatttcgttg
     6961 cgtacttcaa cgagctcgca cccggtccac ccgaacagta cctcgtggac agtgacctcg
     7021 attgggggca ggacctccac cgcctggccg atacgctccg cgcgcgaggg atcggccagg
     7081 ttgccctggc ctataacggc tcggccgatc caggagcggt gggaatcgag ggctaccgct
     7141 ggctccgccc cttcacgcgc gataccggct ggatcgccgt tagcgtgttt gcgctggagg
     7201 tggggatttg gaaccagccc acctgggatg actacgcctg gctgcggagc cggcagccgg
     7261 tggcgcgggc cggtcggtcg atcctgctat acagagtggt gcccgacacc atccagtgag
     7321 agagaccgcg cgccggatcg cactcccgct gggtgtcggc tgcgtggcgg ccgccattgt
     7381 ctgggccagc tttcgaactc ccggggtcgc gtctgatttc gaccacctgt ggatcgctgc
     7441 acgtgccctc acaaggggag atgatccgta ccgggcggtg ctggaggcgg gtaacagata
     7501 cccgttgttc tacccgttgc ccgccgtgct gctggtcttg ccactcgcgg gcttgccggt
     7561 cgggcttgcg cgagtcatct ggtcgggaat cggcgcagct gtgctggctg aagcagcgcg
     7621 ccggtacggg cgcggtcttc cgcccgcgct gctcagcgcc ggtttcgcgg ccgccatagt
     7681 acaggggcag tggtctcctc tgttgaccgc gagcgtagtg cttccggttc tggccgtagc
     7741 ctgggtcgcc aagccgtcga tcgggctcgc ggtcgcggca gccgctccct catggcgcgt
     7801 cgtcgggtgg gcccttgggc tactcgtggt cagcctggcg ctacgaccca catggccccg
     7861 cgggtggatc gacgccgtcc agtcgcagat ttatgtcgcg ccgattctgc ggcccggcgg
     7921 catcatgttg ctccttgccc ttctgcgctg gcgacggccc gagggtcgac tgctcgccgt
     7981 gctcgcctgt gtcccgcaca gccccaccgc atacgaaaca ttgccgctgt tcctgattcc
     8041 gcgaacccgg tgggaggggt acgggctggc ggcgctgggt tacgccgcag ctttcctggg
     8101 tgcctggctc actccctgga gtcacgccat cgagacgttg ccgcaagacc tgtctcgcca
     8161 gtggcctttc atgcttctgc tggtctatct cccagcgctc gtgctggtgc ttcgcttgcc
     8221 caatacccca ggctcggtcg gcgccacctc gtaaccaaac cgatctcgtg cgaccagaat
     8281 ggatttctga atcgcggcga gacaggctac cgagagtcgt tgccaggtcg gggttcgggc
     8341 gactgatcca ggttcaccgg ggtggaggcc gactcgttgg caaactggta ccgggcgact
     8401 accgcataat ccgccaccag cgaatcgcgg cgcacgccac ccgctgagcg gattgcctca
     8461 tcgagcttga tatcggccca agggagcttg atcgtgacga gccacgccgt cggccgcgcc
     8521 gccatcctga ttcgctgggc ctccgatgca gcccagccag gatccgaagg tggcttgtgc
     8581 ctcgcaccga cccgaaaagc cccgccatcg ccgacgccga gcaattcgac gccaccgtga
     8641 aatggaaaaa ccagctcggc gccgtcaccc gagactctcc gccctcttga cctctgattc
     8701 tcgaaggccc cacctccgaa gcttgcttca cgagccatcc tctcgaggcg ggagatgtca
     8761 ggatgggacc agttcgtggt atagaacgtc caggcgggaa gtacaccggc ttcgacatag
     8821 atcggctcgc ctggaagcag cgccgtttga aggaaggcga ctgccgggcg taggtgtccc
     8881 gaccgatacg ggtcgtccgc gcgaacgacg ttccgaagct gtcccgccag gacgatcacg
     8941 ccgaggaccc ccgcaatccc catccgcaat cgccgccgtc ttcggtagag atcggtcgcc
     9001 ccgactacta gcaacgtgag cagaatgggc acggcgaaca gcatcatcct cgcggcgacc
     9061 ggatagagcc caaggcaaga cgcaagcagc gaggccgaaa tcgaacctat gaagaggata
     9121 gcgatggacg gattcgtcct catcaagcgt cgcacaccga gcagcacgag aaaaccgact
     9181 aggcctgccg ttaacaagag gcaaccgttg ccaagaagcg tgtccgcgac ctcggagacg
     9241 tccgacacga cgctggtgaa cagcacctca cgggccgccc catatgcccg tcccaggact
     9301 cccgggaccc aaagtgtcaa gaaccgttcg ctccagaacc aacgcagata tggattcgct
     9361 gacgccgacc gatagaccag ccagtatgct acagcgaacg agagacccca acaaaggaca
     9421 acggcgccaa agacccccgg ctttcgccga aacgtgggat aggaagcagc tgcgattccg
     9481 gcgagaacaa ag
//
LOCUS       DASRJQ010000438         2334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_461708, whole genome shotgun sequence.
ACCESSION   DASRJQ010000438 DASRJQ010000000
VERSION     DASRJQ010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461708"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..1046)
                     /locus_tag="VFB89_09945"
     CDS             complement(129..1046)
                     /locus_tag="VFB89_09945"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HZM27668.1"
                     /translation="MRYRLAIPLALVLNTLACQTERAPLSQLGKDRPSTPAANHAVEE
                     RKTEPFRRNAGLTGSAAAGEPEASPAELPQSLRQSEPWSTMTSPMVIRTGSASIRIDS
                     LELALTTVRGLAGRLGGYVANTSIQAGPAQYRQATLEIKVPAARFDDLVAGLEPIGKV
                     EGVTVAAEDVGEEFVDVSARVTNAKRLESRLIDLLATRTGRLQDVLSVERELARVREE
                     IERYEGRLRYLQTRAATSSLSITVHEPIPVVGRPGSNVLVRAFERAWRNFVELVAAFI
                     ASLGVLVPVTLAGGTVVWGIRRWRRPRLA"
     gene            1157..1813
                     /locus_tag="VFB89_09950"
     CDS             1157..1813
                     /locus_tag="VFB89_09950"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZM27669.1"
                     /translation="MPATVRKPAPTTDPIHVVRGAGQAAALLNPDRVRILEALRDPAS
                     ATALSTRVRMTRQRVNYHLRELERAGLVELVEERRKGNCTERILRASARSFLISPEVL
                     GAVGPAAGATAVQDRFSTAYLITAVARVLRDVALLRHRADEAGKPLATLAFEADITFA
                     SAGDRAAFAEELSGTLAALAAKYHRDQPGGRRFHFVLGGYPAITKREDRSNAEQDSHA
                     "
     gene            1806..>2334
                     /locus_tag="VFB89_09955"
     CDS             1806..>2334
                     /locus_tag="VFB89_09955"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HZM27670.1"
                     /translation="MPDQTRSDEQGTVGGLRTPIEDSVTLDVPPELVWRALTDAEELT
                     RWFPFAATVQPGVGGSVALSWGQGFEKAVIEVWEPDRRLRTVERRRDSAGRVVEIAVD
                     FLIQSEGGKTVLRVVHSGFGASTGWNEEYDGTVRGWRYELRGLRHYLASHVGTNRRVA
                     IAETPTSLSPEASYER"
BASE COUNT          400 a          767 c          764 g          403 t
ORIGIN      
        1 aatgtagcgt gagccctggc atcgcgcggg ccgggccgcg ccctcgaggc acgagtggct
       61 tgggcgctca ctggatcgag agcgccccaa gccactcatg ctcccggacc gcgcgcgatg
      121 cccgggctct acgcgagcct gggcctccgc cagcggcgga tcccccacac caccgtgccg
      181 ccggccaggg tcaccggcac cagcaccccg agtgaggcga tgaaagcagc gaccagctcg
      241 acgaagtttc gccaggcgcg ctcgaaggca cgtaccagga cgttcgaccc cggccgcccg
      301 acgaccggaa tcggctcgtg gacggtgatc gagagtgagc tggttgcggc gcgagtttgg
      361 agatagcgca gccgcccctc gtagcgctcg atttcttccc ggacccgggc cagctcgcgc
      421 tcgaccgaga gcacatcctg caagcgaccg gtacgagtgg cgagcaggtc gattagacgg
      481 ctctcgagcc gcttggcatt cgtcacccgg gcgctcacgt ccacgaattc ctcgccgacg
      541 tcctccgcgg ccacggtcac gccttcgacc ttaccgatcg gctcgagtcc ggccaccaga
      601 tcgtcgaagc gcgctgccgg aaccttgatt tccagagtgg cctgacggta ctgcgcagga
      661 ccggcctgga tcgacgtgtt cgccacgtag ccacccagcc ggccggcgag ccctcgcacc
      721 gtcgtcaggg ccagctccag cgaatcgatg cggatactcg cgcttccagt gcggatcacc
      781 attgggctcg tcatggtgga ccatggctcg ctctggcgaa gggactgagg cagctccgca
      841 ggactggcct ctggctctcc ggctgctgcg cttccggtga gcccggcgtt cctccgaaat
      901 ggttcggtct tgcgctcttc gactgcgtga tttgcggctg gtgtgctcgg gcggtctttc
      961 ccgagctggc tcaacggggc ccgttcggtt tggcatgcaa gagtattgag gaccagcgcg
     1021 agcgggatgg ccaggcggta gcgcacaatt gcctccttga aggtcgcaag gagatgcgaa
     1081 atgctcggaa acttgcacgc cgaccgttaa tcctgtcttg acaagaatag ttgtcggcaa
     1141 tattctgtga tccgtgatgc ctgcaactgt ccgcaagccg gccccgacca ccgatcctat
     1201 ccacgtcgtg cgtggggcag ggcaagcggc cgcgctgctc aatcccgatc gcgtgcgcat
     1261 cctcgaggcc ctccgggatc cggcctcggc cacggctctc tcgacccgcg taaggatgac
     1321 ccggcaacgg gtcaactacc acctgcgtga gctcgagcgg gctgggctgg tcgagctggt
     1381 ggaggagcga cggaagggca attgcaccga gcgcattctc cgcgcttccg cccgctcgtt
     1441 cttgatcagt cctgaggtcc tcggggcggt cggaccggca gccggtgcga cggctgtcca
     1501 ggaccggttc tcaacggcat acctgataac ggcagtggct cgcgtgctgc gtgacgtcgc
     1561 tctgctacga catcgggccg acgaggcggg caaaccgctc gcaaccctcg ccttcgaggc
     1621 cgacatcacc ttcgcatccg cgggtgaccg ggctgccttt gccgaagagc tgagcggcac
     1681 gctggcggcc ctcgcggcca aataccatcg agatcagccg ggcgggcggc gattccattt
     1741 tgtgctcgga ggctatccgg ccatcaccaa acgagaggat cggtccaatg ccgagcaaga
     1801 ctctcatgcc tgaccagaca cgttcggacg agcaaggaac tgtcggtgga ctgcgaactc
     1861 cgatcgaaga ctcggtcacc ctggatgttc cgcccgaact ggtgtggcgt gcgctcaccg
     1921 atgcagagga actaacacgc tggtttccgt tcgctgcgac cgtgcaaccc ggagtagggg
     1981 gcagcgttgc cctgtcatgg ggacaagggt tcgaaaaggc ggtgatcgaa gtctgggagc
     2041 ccgatcgccg gttgcgcacg gtagagcgcc gccgcgactc cgccggccgc gtcgttgaaa
     2101 tcgcagtgga ctttctgatc cagagcgaag gcggcaagac agtcctccgc gtagttcatt
     2161 ctggctttgg tgcgagcact gggtggaacg aggagtatga cggcaccgtc cgcggatggc
     2221 gctatgagct tcgcggtctg cgccactacc tggccagcca cgtggggacc aaccggcgag
     2281 tcgctatcgc ggagaccccc acgtcgctgt cgccggaagc gagctacgaa cgca
//
LOCUS       DASRJQ010000439         2824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1934717, whole genome shotgun sequence.
ACCESSION   DASRJQ010000439 DASRJQ010000000
VERSION     DASRJQ010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1934717"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="VFB89_09960"
     CDS             complement(<1..896)
                     /locus_tag="VFB89_09960"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HZM27671.1"
                     /translation="MRIASLLPSATDVVCALGLEACLVGVSHSCDAPAAARLPRLTTS
                     RLPLNSDSRTIDSAVRESIARGVSLYQIDSRRLRALAPDLVLTQNLCDVCAVGEAEVS
                     DALEAACLAPTVLTLGPKTLGEAWEAIRVIGRATGRFEQADALVSALEHRVAAVAAVT
                     RTFPHRPRVAFLEWIDPPIVGGHWNPELVELAGGQDVLGTPGAPSRTVSWSEVAESEP
                     DLLCVACCGFTAERAGADLDLLLQNPPMRDLPCIRDGRVAVFDGVGLFSRPGPRLAES
                     LELLAAAIHLQLFGASTGSSPTL"
     gene            complement(1086..1769)
                     /locus_tag="VFB89_09965"
     CDS             complement(1086..1769)
                     /locus_tag="VFB89_09965"
                     /inference="COORDINATES: protein motif:HMM:NF014015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27672.1"
                     /translation="MPAERILLSWSGGKDAAWTLRELRSRGNGEVVGLVTTVVPEHGR
                     TAMHGVRLGLIRAQAAATGLPLLEVSLPWPCSNELYEAAFLDGLERARESQGVTHVAF
                     GDLFLADIRSYRERLLAGTGLEPLFPLWHCPTDELLETMLAAGVRARIVCLDPTQLDR
                     RFAGRALDQQLVGELPAHVDPCGERGEFHTFVTAGPMLACPVAVYPGDIVERDGFVYA
                     DFFGAEPDA"
     gene            complement(1773..>2824)
                     /locus_tag="VFB89_09970"
     CDS             complement(1773..>2824)
                     /locus_tag="VFB89_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZM27673.1"
                     /translation="ETSIQRNTVDGRLVFSGLAATTLMGGAVFDDEHEQDFSRSDGDF
                     GPSSDSFEASRTNWGFYLQGTVQPIRQVQLNAGGRLDDNERFGDFWTYRVAALGFITA
                     TTRVRASVGKGFKEPAFFDTYSTSPFAKGNPNLRPERSQNFEAGVEQDLAHGHVMLSV
                     TGFIQRFHDLIQFTAAPANPEDPNFFNVAAADASGVETVLALRHLGPFDASASYTWLH
                     TKVTDAGFDSGPDAQFVDQQRLIRRPDNAVTLRLSSVLDPRVSVGGVLTVIGSRDDLQ
                     FAQFPNPTQRVTLPSYATVDLSARVALLEPHGLTPGLALQARVENLFDEDYQQVAGFL
                     SPGRTVIVGVSSGIH"
BASE COUNT          541 a          932 c          902 g          449 t
ORIGIN      
        1 agagtgggcg aagagcccgt cgaggcacca aagagctgaa ggtggatggc ggcggccagc
       61 aactcgaggc tctccgccag ccgcggccca ggccggctga acaacccgac accatcgaag
      121 accgcaacgc gcccgtctcg aatgcagggc aggtcgcgca tcggcggatt ttgcagcaga
      181 agatcgagat cggcccccgc ccgctccgcc gtgaagccgc agcatgctac gcaaagaaga
      241 tccggttccg actccgccac ctcgctccag ctgacagttc ggctcggcgc acccggagtc
      301 cccaacacgt cctgacctcc agccagttcg acaagctccg ggttccagtg tcctccgacg
      361 atcggggggt cgatccactc caggaatgcc acgcggggcc gatgcggaaa cgtcctcgtc
      421 actgcggcca cggcggcgac gcggtgctcg agggcggaca ccaacgcgtc ggcctgttcg
      481 aagcgccccg ttgccctgcc gatgactcga atcgcttccc atgcctcccc cagtgtcttc
      541 gggccgagcg tcaagacggt cggtgcgagg cacgccgcct ccaacgcgtc actcacctcg
      601 gcttcgccca ctgcgcagac gtcacagagg ttctgtgtga gcactaggtc cggtgccaaa
      661 gctcgcaggc gccggctatc gatctggtag agcgagacgc cccgagcaat cgattcacgc
      721 accgctgagt cgatggttcg actgtcggaa ttcagcggaa ggcggctggt agtcaaccgc
      781 gggagcctgg ctgcggccgg cgcgtcgcag gaatgactca cgcctaccag acaggcttcg
      841 aggcccagcg cgcagaccac gtccgtggca ctcggaagga gcgacgcgat cctcatcgtt
      901 ggcgctctcc aggctcgggg tcgcgaggca cccatgcggc cactcgcgac atcacctttt
      961 gccgggcatc ggcctcgagc cggacccagc caactatctc tgcgatggtg cggccgcatc
     1021 cgtcgcacac gtcatccggt cccaagcggc agatcgaccg acaggggctc gggacccagg
     1081 actcctcatg catcgggttc ggccccgaag aagtcggcgt agacgaagcc gtcgcgctcc
     1141 acgatgtctc ccgggtagac ggcgaccggg cacgccagca ttggtcccgc ggttacaaaa
     1201 gtgtgaaact cgccgcgttc gccgcaggga tcgacgtgcg ccggtaactc gccgacgagc
     1261 tgctggtcga gcgcgcgccc ggcgaaccgg cggtcgagct gcgtcggatc aaggcacacg
     1321 atacgggccc gcacaccagc ggccaacatc gtttctagca gctcgtcggt cggacaatgc
     1381 cacagcggga agagaggctc gagcccggtg ccggcgagca atcgctcgcg atagctccga
     1441 atatcggcga gaaacaggtc gccgaaggcg acgtgcgtga cgccctgact ttcccgggca
     1501 cgctcgagtc catcgaggaa ggccgcctcg tacagttcgt tgctgcacgg ccagggcagg
     1561 gacacttcga gcaaagggag cccggtggca gcagcctgag cccgaatcag cccgagtcgc
     1621 actccgtgca tggcggtgcg cccgtgctcc ggtacgactg tggtcacgag cccgacaacc
     1681 tccccgttgc cgcgcgaccg aagctcacga agagtccagg ctgcgtcctt accaccgctc
     1741 cacgaaagca gaattcgctc tgcaggcatc tgtcaatgaa taccggagga cacgcccacg
     1801 atcaccgtgc gccccggcga caagaatcct gccacttgct gatagtcctc gtcgaacagg
     1861 ttttcgactc gcgcctgcaa ggctaggccg ggcgtcaggc catgcggctc aagcagcgcc
     1921 actcgcgccg aaaggtcgac ggtggcgtag ctgggcagag taacccgttg cgtcgggttg
     1981 ggaaactgcg cgaactggag gtcgtcccgg ctgccgatca ccgtcaatac tccgccgacg
     2041 gagacccggg gatccagcac tgaggagagc cggagcgtca ccgcgttatc cgggcggcga
     2101 atcagtcgct gctgatcgac gaattgggcg tcgggtccgc tatcgaagcc ggcatcggtg
     2161 acctttgtat gcagccaagt atacgaggcg ctcgcgtcga aaggaccgag atgacggagc
     2221 gcaaggacgg tctccacccc ggaggcatcg gccgcggcga cattgaagaa gttgggatct
     2281 tccggattcg ccggagctgc agtgaactgg atgaggtcgt ggaagcgctg gatgaagccg
     2341 gtcaccgaca gcatgacgtg cccgtgagcc agatcctgct cgactccggc ttcgaagttc
     2401 tgcgagcgtt ccgggcgaag gttgggattc cccttggcaa aaggactggt cgaataggtg
     2461 tcgaagaagg ccggttcctt gaatcccttg ccaactgagg ctcgcacccg cgttgtcgcg
     2521 gtaatgaagc cgagcgcggc tacccgatag gtccagaagt cgccgaatcg ctcgttgtca
     2581 tccagccggc cgccggcatt gagctgcacc tgccggatcg gctgcactgt gccttgcagg
     2641 tagaaccccc agttggtccg actcgcctcg aatgagtcgc ttgagggtcc gaagtcaccg
     2701 tcagaacgcg agaaatcctg ctcgtgctcg tcgtcgaaga cggcgccgcc catcagggtt
     2761 gtcgccgcaa gccctgaaaa caccagtcgt ccgtcaacgg tattgcgctg aatggacgtc
     2821 tcac
//
LOCUS       DASRJQ010000440         4669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2545029, whole genome shotgun sequence.
ACCESSION   DASRJQ010000440 DASRJQ010000000
VERSION     DASRJQ010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2545029"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VFB89_09975"
     CDS             complement(<1..477)
                     /locus_tag="VFB89_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZM27674.1"
                     /translation="MKPRVKRRAVRPAQIARAVDEGRESLDLYLDEISRVPLLNRDEE
                     MELARKAFKGSIAAQERLARHNVRFVVSVAKKFQNRGVPLVDLIGEGNLGLMTAARKF
                     DPDRGVKFISYAVWWIRQAVQAAIARHGRPVRVPLNRTADLSRLGRTTTLLKDKLGR"
     gene            726..1874
                     /locus_tag="VFB89_09980"
     CDS             726..1874
                     /locus_tag="VFB89_09980"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HZM27675.1"
                     /translation="MRIAVPKESAPRELRVALVPESCKKLVQAGYEVAIEREAGLAAG
                     FPDGAYQDAGAVIESDLRGLLQSAGLVLRVNAPQIDRPDEISAMRQGTILLGSLMPLR
                     NLAVVRSLAAARITAFSTDAIPRTTRAQSMDTLSSMANIAGYKGALMAALELPKYFPM
                     LMTAAGMVRPARVFVIGAAVAGLQAIATARRLGATVSATDVRPEVKEQIESVGGKYVG
                     IELEESAAAGGGYAKELSEEDRRRQRELLAEQCAQADAVITTALIGGVFAPKLIGADI
                     VKSMKPGSVIVDLAADGGGNCELSRPGETVRVGGVTIVAPLNLAATMPSDASLLFSRN
                     LNAFVQAFTVDKAFRLDPDDDIQQGALITHDGAVTHARTREALQKESA"
     gene            1871..2203
                     /locus_tag="VFB89_09985"
     CDS             1871..2203
                     /locus_tag="VFB89_09985"
                     /inference="COORDINATES: protein motif:HMM:NF024179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HZM27676.1"
                     /translation="MTQDAAPVQVADFWSQLFVLVLATFIGLGVIRRVSRLLHTPLMS
                     ITNAISAIAVVGSIIVTGADYPAHIRLLGAIALFASMTNIVSGFLITDRMLKMFKTER
                     KPTAGEPK"
     gene            2200..3609
                     /locus_tag="VFB89_09990"
     CDS             2200..3609
                     /locus_tag="VFB89_09990"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HZM27677.1"
                     /translation="MSQGIVHFAYILATVLFIFALHWMNDPKTARQGVMAGVVAMAIA
                     VLVTWAQPEVIHHGWIVLAIVAGFIVGIPLARVPLTAVPQRTALSHAFGGLAAGLVGT
                     AKYYLWFGEGPENLTAFRMIAIIAEIILGYLTFTGSLMAAGKLQEVKWIPQRPVTYPG
                     QNFTNLGLLLIAAGLGIALILHPTADWAPKLFPVIIGLALLFGVLLIIPIGGADMPTV
                     IAILNSYAGLSAVAMGFVLDNKLLITAGALDGSSGLILAIIMCKAMNRSFTNVLFGAF
                     GQVQQIAAGGDHKVYKSETIEGAGQVMEQANQVVIIPGYGMAVAQAQHKVRELYDQLK
                     KRGITVKFAIHPVAGRMPGHMNVLLAEADIPYTDLVEMDEINPDMPQVDVALVVGAND
                     VVNPAARSEKTSPIYGMPIIDADRARTVFAIKRSKNPGFAGIDNELYFSDKTWMLFGD
                     AKNVIGELVKTLGGGSGLH"
     gene            3684..>4669
                     /locus_tag="VFB89_09995"
     CDS             3684..>4669
                     /locus_tag="VFB89_09995"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HZM27678.1"
                     /translation="MKSAPQVHKFGGASLADAAAIGQAVAIVRSHRAEPTVVVVSAMA
                     GVTDQLLAIASHAVRGDTARVSELIDGLQARHAQAARVILPAGNARASLITHISQQFA
                     ELAALAAGLGLLRDLTPRTSDVILARGERLSARLITAALAVHRIRARYVDATTIVRTD
                     AHFGHASPDYERTDRAARRVLRPLLAQGDVPVIPGFLGASPEGDLTTLGRGGSDLTAT
                     LLARAIGAGRVSLWKDVPGLLTADPKVVPDARVIPALHSREAAELAYYGAKVLHPRAL
                     IPLAGRKIPLFVRPFADPASPGTEVSDRVAATRLPVKALSAATHQTLVTVAG"
BASE COUNT          809 a         1522 c         1485 g          853 t
ORIGIN      
        1 acggcccagc ttatccttga gcagggtggt ggtgcgaccc agccggctca ggtcggcggt
       61 acggttgagc ggcactctta ccgggcggcc gtgccgcgcg atggcagcct gcaccgcctg
      121 ccggatccac cagactgcgt aagaaatgaa tttgacgccc cggtcgggat cgaacttccg
      181 agcggcggtc atgagtccga ggtttccttc gccgatgagg tcgacgagtg gtacgccccg
      241 attctggaat tttttcgcca ccgaaacgac gaaccgaacg ttgtgacgcg ccagccgttc
      301 ctgggccgcg atgcttccct tgaatgcctt gcgggccaac tccatttctt catcgcggtt
      361 caagagaggg acccggctga tttcatcgag gtagagatcg aggctttccc ggccttcatc
      421 gacggcccgg gcgatctggg ccggccggac cgcgcgacgc ttgacacgtg gcttcacggt
      481 cactctcgga acctcctatg ggagcaggac ccttgtaagg ggtcgtacgg cgggggggcg
      541 tatcaaaatc ggtgggcgaa tataacgtct aagctacgga aaatcaatgg cttagcgtaa
      601 gctactgtgc tgatatgatt tgcacaagtt tcccacattg tttccacgac gccggatcgg
      661 cttgaccgca cggcgaccgt gtgaagatta gtcgtccgaa ctctcccgca gccccggagt
      721 cccggatgcg aatcgctgtt cccaaggaga gtgcgccgcg cgagctccgc gtcgcactgg
      781 tccctgaatc gtgcaagaaa ctggtccaag ccgggtatga ggtcgctatc gaacgcgagg
      841 ccgggctggc ggccgggttt cccgatggcg cataccagga tgccggtgct gtcatcgagt
      901 cggacttgcg ggggctgctc cagtccgccg gcctggtgct ccgagtgaac gcaccgcaga
      961 tcgaccggcc cgacgagatc agcgcgatgc gccaaggtac cattcttctc ggttcgctga
     1021 tgccgctccg gaatctggct gtggttcgct ctctcgccgc tgcccgaatc acggcgttct
     1081 ccaccgatgc gatcccccgg accacccggg cacagtccat ggacacactc tcgtccatgg
     1141 cgaacatcgc tgggtacaag ggggctttga tggctgcact ggagctgccc aagtacttcc
     1201 cgatgctcat gacggcggcc ggcatggttc gtccggcgcg cgtatttgta atcggtgccg
     1261 ccgtggccgg ccttcaggcc atcgccaccg cacgccgatt aggggccacg gtctccgcca
     1321 cggacgtgcg ccccgaggtg aaagagcaga tcgagagcgt gggcgggaaa tacgtgggga
     1381 tcgagcttga ggagagtgcg gcggcaggcg gcggttacgc caaggagctt tccgaggaag
     1441 accgccgccg ccaacgggag ctgctcgccg agcagtgcgc ccaggccgac gccgtgatca
     1501 ccaccgcgct gatcggcggg gtgttcgccc ccaagctgat tggcgcggac atcgtgaaat
     1561 ccatgaagcc cgggagcgtg atcgtggatc tcgccgccga cggcggcggc aactgcgagc
     1621 tgtcccgccc gggggagacg gtccgggtcg gcggggtgac catcgtggcg ccgctcaacc
     1681 tcgccgccac gatgccttcc gatgcgagcc tgctcttttc acggaatctc aacgcgttcg
     1741 tgcaggcctt caccgtcgac aaggcattcc ggctcgatcc cgacgacgac atccagcaag
     1801 gggcgttgat cacccatgac ggcgcggtga ctcacgcccg tacccgcgaa gccctccaga
     1861 aggagagcgc atgacccaag acgccgcgcc ggttcaggtc gccgacttct ggtcgcagct
     1921 tttcgtactc gtcctcgcga cgttcatcgg actgggcgtg atccgccgag tttcccggct
     1981 gctccacacg ccgctgatgt cgatcaccaa cgcgatctcc gcgatcgcgg tggtggggtc
     2041 gatcatcgtg accggtgccg actacccggc ccacatccga ctgctcggtg ccatcgccct
     2101 gttcgcctcg atgaccaaca tcgtgagcgg atttctaatc acggaccgga tgctcaagat
     2161 gttcaagacc gagcggaagc ccaccgccgg agaaccgaaa tgagccaggg catcgtccac
     2221 ttcgcctaca tcctggcgac agtgctgttc atcttcgcgc tgcattggat gaacgacccg
     2281 aagaccgcac ggcagggcgt catggccggg gtggtcgcca tggccatcgc ggtcctcgtc
     2341 acctgggctc agccggaagt gattcatcac ggctggatcg tgctcgcaat cgtggcggga
     2401 ttcatagtcg gcatcccgct ggcgcgggtg ccgctcacgg cagttcccca gcggaccgcg
     2461 ttgtcgcacg cgttcggcgg tctcgcggcc ggcctggtgg gaacggccaa gtactacctc
     2521 tggtttggag aggggccgga aaacctcacc gctttccgca tgatcgccat tatcgcggaa
     2581 atcatcctcg gctatctcac gttcaccggc agcctcatgg ccgcgggcaa gctgcaggaa
     2641 gtgaaatgga ttccgcagcg acccgtgacc tatccggggc agaacttcac caacctcgga
     2701 ctgctgctga tcgcggctgg actgggaatc gcactgatcc tgcaccccac cgctgactgg
     2761 gcgcccaagc tgtttcccgt catcatcggc ctggcgcttc tgttcggagt gctcctgatc
     2821 attccgatcg gcggcgctga catgccgacg gtcatcgcga tcctcaactc atatgccggc
     2881 ctgtcagcgg ttgcgatggg gtttgtcttg gacaacaagc tgctgattac ggccggcgcg
     2941 ctcgacggct cgagcggtct cattctggcc atcatcatgt gcaaggccat gaaccgctcg
     3001 ttcaccaacg tgctgttcgg cgcctttggg caggtccagc agattgccgc tgggggcgac
     3061 cacaaggtct acaagtcgga aacgatcgag ggtgccgggc aggtgatgga acaggccaac
     3121 caggtcgtca tcatccccgg ctacggtatg gccgtggccc aggcgcagca caaggtccgc
     3181 gagctgtacg accagctcaa gaaacgcggc atcacggtga aattcgcgat ccatccggtt
     3241 gccggacgga tgccggggca catgaacgtc ctgctcgcgg aagccgacat cccctatacc
     3301 gacctggtcg agatggacga gatcaatccg gacatgccgc aggtggacgt ggcactcgtg
     3361 gtaggtgcga acgacgtggt gaatccggcg gcgcggtcgg aaaagacgag tcccatctac
     3421 ggcatgccga tcatcgacgc ggatcgtgcc cggaccgtgt tcgcgatcaa gcgcagtaag
     3481 aaccccggct tcgccgggat cgacaacgag ctgtacttct ccgacaagac ctggatgttg
     3541 ttcggcgatg cgaagaacgt gattggagag ctggtgaaga cgctcggcgg cggttcaggc
     3601 ttgcactgac ctgcgcgagc ggtccgcgcc aacgttgcac tttatgcgcc ccgcggtata
     3661 tcctccgcga caccatgccg ccagtgaaat ccgccccgca ggtgcacaag ttcggcgggg
     3721 cctccctggc cgacgcagcc gcgatcggcc aggctgttgc tatcgtccgc agtcatcgcg
     3781 ccgagcccac agtcgtcgtg gtatcggcga tggcgggtgt aaccgatcag ctcctcgcga
     3841 tcgcgtcaca cgcggttcga ggagacactg ctcgggtctc ggaactgatc gatggtctgc
     3901 aagccaggca tgctcaagcg gcccgggtca tcctgcccgc gggcaatgcc cgcgcctccc
     3961 tgatcacgca tatatcccag cagttcgccg agcttgcggc gctcgccgcg ggtctgggac
     4021 tgcttcgcga cctcactccg cgcaccagcg acgtgatcct ggcccggggg gagcggctga
     4081 gcgcgcgtct gataacggct gccctcgcag tgcaccgcat tcgagcacgc tacgtcgacg
     4141 cgaccacgat cgttcgcact gacgcgcatt tcgggcacgc ctcgcccgat tatgaacgca
     4201 ccgatcgcgc ggcgcggcgc gtcctgcgcc cgctactcgc gcaaggcgat gtcccggtga
     4261 ttccgggttt tctcggcgcc tctcccgagg gcgatctcac cacactcggt cggggcggat
     4321 ccgatctcac cgcaacgttg ctggcccggg ccatcggagc cggccgagtg tcgctctgga
     4381 aggatgtgcc cgggctgctg accgcggacc ccaaggtcgt gcccgatgcg cgggttattc
     4441 cggcgctgca cagccgcgag gccgccgagt tggcgtacta tggcgccaag gtgctccacc
     4501 cgcgcgcgct gattccgctc gcgggacgca agattccgct gttcgtgcgg cccttcgctg
     4561 atccggcatc ccccggaacc gaagtttccg atcgcgtcgc cgcaacccgg cttccggtga
     4621 aggccctgtc ggcagcaaca catcagaccc tggtgacggt ggccggaaa
//
LOCUS       DASRJQ010000441         2549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1164505, whole genome shotgun sequence.
ACCESSION   DASRJQ010000441 DASRJQ010000000
VERSION     DASRJQ010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164505"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1676)
                     /locus_tag="VFB89_10000"
     CDS             complement(<1..1676)
                     /locus_tag="VFB89_10000"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZM27679.1"
                     /translation="MPRPRYFVTIMGRLLLLPALFVGLLSCGGGSGSVTTSSTTTPEL
                     VQTTGSLPQPPQAKGSDLSTNSALDWANRVTSNDPKVRAIAEAAVVQGAERSLPLLRR
                     FLNGRNEDLHRESLEIIRRIGPPAIPLLVDLLRDARVSIRRSAADALIDLAPDTERIQ
                     PALRRALRDADSQVAGDAARALGALRNRASPSIRALVEALSHEDPYVRVYAAEALASI
                     GPKAAAATRALARALGDAIPGVRWAAAEALATIGPAAQSAVPQLIAALKDEFLYVRIC
                     AAGALGSIGPKAQTAREALRAAANDPTLRTEAEWALNRIAGVESGEPVAPALPAPTVA
                     PQHQTMVAQTGNPPVDWNTTTGRNIVWSVELGNETFGRPVVAGDAVYVGTNNARHMNP
                     AFQDESGVLMAFRVKDGAFLWQDVAPRVDRGLREFLLPSTTSAPYVEGNRLYYVTAEC
                     QLRCLDTLGFRDGENSGPYREEVFRDNAAADIAWELDMCGRLGVFPHEASNSEVLPVG
                     DLLMVSTSNGQNEGHTRVPSPRAPSLIAVDKRSGEVVWRAIGPGEQVLHG"
     gene            1822..>2549
                     /locus_tag="VFB89_10005"
     CDS             1822..>2549
                     /locus_tag="VFB89_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27680.1"
                     /translation="MVDSIRRKLLKAGAAAAAMSATPSLLAQQSANRDSGRFYEKGNV
                     RIRYEETGSGFPLLLIAGGGLNGSTIAGIRGGNPFNTIDAFKGEFRCIYADLRNSAGD
                     SAGPLEIERPWDSYTDDHIGLMDHLRIDKFMVLGFCIAGPFIWNLIKRAPTRVVAAVP
                     AQPVGFRPELPNALYDSQKTGWADPLVRRRSDITAATADQFLTRMFRNNPDFVLTVTR
                     DFVKSCQTPVLVLPDDTAGHSLTVA"
BASE COUNT          467 a          830 c          809 g          443 t
ORIGIN      
        1 tggccgtgca gcacctgctc gcccgggccg atggcgcgcc ataccacttc gcccgaacgc
       61 ttgtccaccg cgatcagact tggggcgcgc ggcgatggga cgcgcgtatg gccttcattc
      121 tggccgttgg aggtagagac catcagcaga tcacccacgg gcaacacttc gctgttggag
      181 gcttcatgcg gaaagacacc caggcggccg cacatatcca gttcccaggc gatgtcggcc
      241 gcggcgttgt cccggaagac ctcctcccga tatggaccac tgttctcgcc gtcgcggaaa
      301 cccagcgtgt caaggcaacg gagttggcac tcggcggtga catagtagag tcggttccct
      361 tcgacatacg gcgcgcttgt cgtcgaaggc agcaggaact cccgcaggcc acggtccact
      421 cgcggcgcca cgtcctgcca aaggaacgcg ccatccttca cgcgaaaggc catgaggacg
      481 ccgctctcgt cctgaaaggc ggggttcatg tgcctggcgt tgttggtccc tacatagacc
      541 gcatcgccgg caaccacggg acggccaaac gtttcgtttc ccaactcgac gctccagacg
      601 atgtttcgcc cggtggtcgt gttccagtca accggaggat tgcccgtttg agccaccatc
      661 gtttggtgtt ggggcgcgac cgtaggcgcg ggcagggctg gggcgacagg ctctccagac
      721 tcgacgccag cgattcgatt caacgcccac tcagcttcgg tgcgcagtgt gggatcgttt
      781 gcggccgctc tcagcgcctc ccgggctgtc tgtgccttcg gcccgatgct gcccagcgcc
      841 ccggccgcgc agatgcgcac atagaggaat tcatccttca gcgctgcgat caactgcggc
      901 acagcggact gcgccgccgg gccgatggtt gcgagggcct cagccgccgc ccatcggacg
      961 ccgggaatcg catcgcccaa ggctctggcg agggccctgg tcgccgcggc cgccttcgga
     1021 ccgatggacg ccagggcctc cgccgcgtag acgcggacgt atgggtcctc gtgcgagaga
     1081 gcctcgacga gagcgcgtat cgaaggactg gcccgattgc gcaaagcgcc cagggcacga
     1141 gcggcatcgc cagcgacctg cgaatccgcg tctctcagcg cccgtctcaa cgccggttga
     1201 atcctctctg tatcgggcgc aaggtcaatc aaggcatcgg cggcgctgcg acggatcgag
     1261 acccgcgcat cacgaagcag atcgaccagc agcggaatgg ccggcggacc gatccgccgg
     1321 atgatttcca aactctcccg atgcaggtct tcattgcgac cgttgaggaa tcgtctcagc
     1381 aacggcagcg aacgctccgc cccctggacc acggcggctt cggcgattgc ccggactttc
     1441 gggtcgttgg acgttacgcg attggcccag tcaagggcgg agttggtcga gagatccgaa
     1501 cccttggctt ggggcggctg agggagtgaa ccggtggttt gaacgagttc cggtgtcgtc
     1561 gtactgctgg ttgtcacgct gccactgccg ccgccgcacg agaggagacc aacgaacagc
     1621 gcaggtagga gcagaagacg gcccataatg gtcacgaaat accgtggtct cggcacgcaa
     1681 ccgaagtaat tccctcaaga gatgcgtgcc ttccaatgca gctggctacg agagttcagc
     1741 gatcgtgccg actcttccag cagcgttaga atgaccgcga ccccgccatc gaggcgtaac
     1801 gccgggctga gaggaggacc cgtggtggat tcgatcagac gcaagcttct gaaggctggc
     1861 gccgcggccg cggcgatgtc cgcgacgccg tcactgctcg cgcagcagag tgcgaacaga
     1921 gacagcggac gcttctacga aaaagggaat gtccgcatcc gttacgagga gaccgggtcg
     1981 ggcttcccgc tcttgctgat cgccggcggg gggttgaatg gctcgacgat cgctggcatc
     2041 cgcggcggca accccttcaa caccatcgac gcattcaaag gagagttccg ctgcatctac
     2101 gccgacctgc gcaactcggc cggcgactct gccgggccgc tcgagatcga gcggccgtgg
     2161 gattcctaca ccgacgatca tatcggcctg atggatcacc tgcggatcga caaattcatg
     2221 gtgctcggct tctgcattgc ggggcctttc atctggaacc tcatcaagcg cgcgcccact
     2281 cgcgtcgtcg cggcagtgcc ggcgcagccg gtcgggttcc ggcccgagct accgaacgcc
     2341 ctgtatgaca gtcaaaaaac cggctgggcg gaccccctgg tcaggcggcg ctccgacatc
     2401 acggctgcaa cggctgacca attcctgacc agaatgttcc ggaacaaccc cgacttcgtg
     2461 ctcaccgtga cgcgcgactt cgtgaagagt tgtcagacgc cggtactggt cctgccggac
     2521 gataccgcgg ggcattcatt gaccgttgc
//
LOCUS       DASRJQ010000442         4320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_27679, whole genome shotgun sequence.
ACCESSION   DASRJQ010000442 DASRJQ010000000
VERSION     DASRJQ010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27679"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2956
                     /locus_tag="VFB89_10010"
     CDS             <1..2956
                     /locus_tag="VFB89_10010"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HZM27681.1"
                     /translation="DSPHLDRRDFLKAAGAMTAGAAIGARLGIVTQGAAPQVSAPARA
                     VHPFPLSQVTLGDGLFREKRDRMLNYARNYGGNSDVFAGPDRMLCNFRANAGIDTKGA
                     QPPGSWDNATGYLRGHYSGHFMSLLAQAYAGSGDEIFKRKLDYIVAALGECQEALAAA
                     ARRPTPRVPGRFGQALRLTGSPLGAAEHVSLPAGIVSGLDDFTIAMWVNPSVYERRNL
                     PDANPNTDPATLDNSAAILDFGNPNPVFATPPQAHMFLTLRVSDKSPVPGFAMTTTGN
                     DGEQRIEGTSPLPAGQWTHIAVTRSGNTGTLYIDGAVAGTNTSMTLRPADLGVTAGNW
                     IGRCQFPQRNVSYLNAAIDEFQIYDRALSVAELRSLMDSPGGTPGGGNVAWYRFDETG
                     GKEALDSSGRARHGTIIGPNDGRRHPGFLSAYPETPFIRLEEFATYGGNQGIWAPYYT
                     LHKILAGLLDAHVLAGNAQALQIAAKIGDWVYSRLSPLRQEQLDRMWNTYIAGEYGGI
                     NESLAQLHSLLPGRGEYLAAATRFDNAVVFNAMVRDEDVLDGRHANQHIPQFTGYLRI
                     FEQSGESRYFDAARNFWNMVVPHRIYSHGGMGVGEIFRRRDVIAGSLYQFPNENNHAE
                     TCPLYNMLKLSRNLFFHDPDPRYMHYSEQGLYNQILGSRRDVDSSDSPQVAYFVPVRP
                     GQRRTYGNVGTCCGGTGMENHTKYQESIYFRSRDDSALYVNLYADSRLRWPERGFTVT
                     QATKYPAEGESRITVDGSGPLEIKLRVPAWVGRNYAVRINGVVQDLAGSPGAYITLNR
                     QWRPGDTIDISMPFRFRVERALDSPAVQSIFYGPILMAVQGGAVGKSLESGLMNFSFY
                     RYFKLDGDLARAMTPTDKPQHFTTNGQTLAPFYIADPGPSETSPYHLYIRRHEPSIVF
                     GSIDSAVANRSSDDGFTFLDMVWNQAPFASHAQLLAATDRVSLDWRSQGLFSERERVA
                     IRDAAERARAEL"
     gene            3119..3907
                     /locus_tag="VFB89_10015"
     CDS             3119..3907
                     /locus_tag="VFB89_10015"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HZM27682.1"
                     /translation="MKRTYLSGVIAAAVAVTAFVFIPLAGRIAYAQHNSNCQGLPSAA
                     DVRQQLVAAPGMTPGQDGIGGTVGGLFNGTRMWGAIVDRSGEVCSYGTSTADVTQVWP
                     GSQAIAKAKAYTANAFSVDVLALSTARLYTFTQPGHSLWSLGQSNLFNADLLVAPGGT
                     GDKNQIAGGLIFFGGGVPLYEDGKIVGGLGISGDTSCADHEIAKRVRHLLHMDPPGGA
                     TADDIVYSPTPATASVFAHPVCLNTFLNGVPVGGEAPATYPKAP"
     gene            4141..>4320
                     /locus_tag="VFB89_10020"
     CDS             4141..>4320
                     /locus_tag="VFB89_10020"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6597 domain-containing transcriptional
                     factor"
                     /protein_id="HZM27683.1"
                     /translation="MERGFRHYTPRWPLTDYVEGFWSHGHYAQPHGRERVMPTGTMNL
                     VFRCDGSGGASVMLSG"
BASE COUNT          831 a         1388 c         1322 g          779 t
ORIGIN      
        1 cgactcgcca cacctggacc ggcgcgattt cctgaaggct gccggagcga tgaccgctgg
       61 cgccgcgatc ggcgcgcggc tgggcatcgt gacgcagggc gcggcgccgc aggtgagtgc
      121 acccgcacgc gccgtgcacc ctttcccact gagccaggtg acgctcggag acggtctctt
      181 ccgcgagaag cgggaccgca tgctcaacta cgcccggaac tacggaggca atagcgacgt
      241 cttcgcaggc ccggacagga tgctttgcaa cttccgcgcg aacgcgggaa tagataccaa
      301 gggtgctcaa cctcccggca gctgggacaa cgcaaccggt tacctgcgcg gtcattacag
      361 cggtcatttc atgagcctgc tggcccaggc ctacgccggc agcggtgacg agattttcaa
      421 gcggaagctc gattacatcg tggctgcact tggcgaatgt caggaggcgc tcgctgccgc
      481 ggcaagaagg cccactcctc gcgtcccggg ccggttcgga caggcgctgc gcctcacggg
      541 ttcgccgctc ggtgcggccg agcacgtctc gctgccggcg ggcatcgtca gcggcctgga
      601 tgatttcacg atcgcgatgt gggtgaatcc gtcggtctac gagcggagaa acctcccgga
      661 cgccaatccc aataccgatc cggcgacgct cgacaactcc gcagcgatat tggatttcgg
      721 caatccgaac ccggtcttcg ccactccgcc gcaggctcac atgttcctga cgctccgggt
      781 atcggacaag agtcctgtac cagggtttgc gatgacgacc accggcaatg acggcgagca
      841 gcgaatcgag gggacgtccc cgctccccgc aggtcaatgg acgcacatcg ccgtcactcg
      901 ctcaggaaac accggaacgc tctacatcga cggcgcagtc gcaggcacga ataccagcat
      961 gacgctgcgg cctgccgatc ttggtgtaac ggcgggcaac tggatcggca gatgtcagtt
     1021 tccccagcgc aatgtgtcct atctcaatgc ggccatcgac gagttccaga tctacgaccg
     1081 cgctctgagc gtggcggagc tgcggtcgct gatggactcg cccggcggta cgccaggcgg
     1141 aggaaacgtg gcctggtatc gcttcgatga gaccgggggc aaggaggccc tcgattcctc
     1201 cggccgcgcc cgccacggga cgatcatcgg tccgaacgac ggccggagac atccgggatt
     1261 tctttctgcg tatcccgaga ctccgttcat ccggttggag gagttcgcaa cgtatggcgg
     1321 caaccagggg atatgggcgc cctactacac gcttcacaag atcctggctg gactcctcga
     1381 cgctcatgtt ctcgccggca acgctcaggc gctccagatt gccgcgaaga ttggcgactg
     1441 ggtctacagc cggctctcac cgctgcgcca ggagcaattg gacaggatgt ggaataccta
     1501 tatcgccggc gaatacggcg gtatcaacga gagcctggca cagcttcact ccctgctccc
     1561 cggcaggggc gagtatctgg ccgccgcaac gcgcttcgac aacgccgtcg tcttcaacgc
     1621 catggtccgc gacgaggacg ttctcgacgg caggcacgcc aaccagcata tcccgcagtt
     1681 cactggctat ctgcgaatct tcgagcagag cggcgagtcg cgttacttcg acgcggccag
     1741 gaatttctgg aacatggtcg tgccgcatcg catctacagc cacggcggca tgggcgtcgg
     1801 cgaaatattc cggcgccggg atgtcattgc cggatccctt taccagttcc ccaacgagaa
     1861 caatcacgct gagacctgtc cgctgtacaa catgctcaaa ctgagccgaa acctgttctt
     1921 tcacgatccc gatccgcgat acatgcacta ctccgagcag ggcttgtaca accagatcct
     1981 gggttctcgc agagatgtcg atagttcaga cagtccccag gtcgcctatt tcgtgccggt
     2041 gcgtccaggc cagcggagaa cctatgggaa cgtgggcacg tgctgcggag gcacgggcat
     2101 ggagaaccac accaagtacc aggagtccat ctatttccgc tctcgcgatg attcggcgct
     2161 gtacgtcaat ctctacgccg actcgaggtt gcgctggccc gaaagaggtt tcacggtgac
     2221 ccaggccacg aaataccccg ccgagggtga aagccgaatc accgtggatg gcagcgggcc
     2281 gctcgaaatc aaactacgcg tgccggcctg ggttggaagg aactacgcgg ttcgaatcaa
     2341 tggcgtcgtt caggacttgg cggggagtcc aggagcgtac atcacgctca accggcaatg
     2401 gcggccgggt gacaccatcg atatctcgat gcccttccgc tttcgcgtgg aacgtgcgct
     2461 cgatagtcct gccgtccagt cgatcttcta cggtccgatt ctcatggccg tgcaaggcgg
     2521 tgccgtggga aagagcctgg agtcgggact gatgaacttc tcgttctatc ggtacttcaa
     2581 actggatggc gatctcgctc gggccatgac gcccacagac aagccgcagc atttcacgac
     2641 gaacggacag acgctggccc cgttttacat cgccgatccc gggccctcgg agacgagccc
     2701 gtaccatctc tatatcagac ggcacgagcc cagcatcgtg tttggttcga tcgactccgc
     2761 cgtggccaac cgctccagtg acgatggctt tacgttcctg gacatggtgt ggaaccaggc
     2821 gccgtttgcg agccacgcac agctcctagc cgcaacggac cgggtctccc tggattggcg
     2881 cagccagggt ctgttcagcg aacgcgaacg cgtcgcgatc agggatgctg cagaaagggc
     2941 gcgcgcggaa ctgtaggggc tcagcgcgcg acattactcg caattacctt ttcgtaatcg
     3001 ctcgaaaacc caccgcatca cggcgttatg tagtgacacc cggcggcact gcccaataag
     3061 atggcggctt cacatcaagc cttcaggcgg cacgcacgcg cccttcagga gccgaacgat
     3121 gaaacgcacg tacttgagcg gcgtaatcgc cgctgccgtt gctgtcacag cattcgtgtt
     3181 cataccactc gcaggacgca tcgcgtacgc ccaacacaac tccaattgcc aagggctgcc
     3241 gtcggcggcg gacgtgaggc aacagcttgt cgctgcgccg gggatgacac ccgggcagga
     3301 cggcatcgga ggcacggtcg gcggtctctt caacggcacc cgcatgtggg gagccatcgt
     3361 ggaccgctcc ggcgaggtgt gttcgtatgg gacgtccacg gcggatgtga cgcaagtctg
     3421 gccgggcagt caggcgatcg caaaagccaa ggcctacacg gcgaacgcgt tcagtgtcga
     3481 cgtgctcgcc ctctccacgg cgcgtctcta cacgtttacg cagcccgggc actcgctgtg
     3541 gagtctgggt cagtcgaatc tgttcaacgc ggacctgctt gtcgctccag gcgggaccgg
     3601 tgacaagaat cagattgccg gcggcttgat cttcttcggc ggcggcgtcc cgctttacga
     3661 ggatggcaag attgtcggcg ggctggggat cagcggcgac acctcgtgcg ccgatcatga
     3721 gatcgccaag cgagtgcggc acctgctgca catggatcct cccggtggag cgacggctga
     3781 cgacatcgtc tactcgccaa caccggccac cgctagcgtg ttcgctcatc ccgtgtgcct
     3841 gaacacgttt ctcaacggcg ttccggtcgg cggcgaagca ccggcgacct atcccaaggc
     3901 tccatagacc ccgcaccatc gaacgggcgg gcccgagagg acccgcccct cctatccccg
     3961 ggttccaggt ctggtccgaa cgtgccgttt cgctctgatc gcataacgag gatacgtgaa
     4021 ctttcgcgag gaggccgttt atagttcctc tcgtccccgg tgataaggac gtggcgcctc
     4081 gcgactgatt cgaggctcga acggcctacc gccgctgcga ccaaccggcg ggagcacgtc
     4141 atggaacgag gatttcgcca ctacacgccc cgatggcctc tcaccgacta cgtcgagggc
     4201 ttctggagcc atgggcacta cgctcagcct cacgggcgcg agcgcgtaat gcccacggga
     4261 accatgaacc tcgtcttccg ctgcgacggc agcggagggg caagcgtcat gctctcgggc
//
LOCUS       DASRJQ010000443         3223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3482888, whole genome shotgun sequence.
ACCESSION   DASRJQ010000443 DASRJQ010000000
VERSION     DASRJQ010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3482888"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="VFB89_10025"
     CDS             complement(<1..820)
                     /locus_tag="VFB89_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27684.1"
                     /translation="MTLDNSSASGTSVLKRLREDRPLVAVELRPPRSGMSYAESVDAW
                     IDMYHSIRRLARQDTLIFLTDNAVGRSEEENLAHLTANLAGELPAERLVPFLTCKHSL
                     EYIQLYAARAASHGFEALTVVGGDQATGTTRIVPHAYQLRRMMRGWVPRLNLGGWINP
                     HRDAERQVDFLLDRDFEADYYVTQIVSHHHLDQVERFLAESRRRGVPFPGVFGVFLYR
                     SANPRTLRQLGDFFPVPVEGVTRDFEAGLAPEAIVAKTICALREIGVDKIYVSNL"
     gene            954..1586
                     /locus_tag="VFB89_10030"
     CDS             954..1586
                     /locus_tag="VFB89_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27685.1"
                     /translation="MTPQWMAVLMLTAACATVGKLGAPATPYRTDPPRVGEIPDPELI
                     DVIRKGDLIVLGTPVDRVSEAGFYTPKFQMGARETWYSVKIAVDSVVKGKLRSAKKVD
                     LGMLPVWMNPGPPFPSLEANEIVVQYPETESPNMHWGLAPVLTLGERAVFIFKKCWYC
                     LPLTGVANPRGPYYVANPWVAMSWGSKLPPDEWSRVVRLTESLRSPRASR"
     gene            complement(1591..3042)
                     /locus_tag="VFB89_10035"
     CDS             complement(1591..3042)
                     /locus_tag="VFB89_10035"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZM27686.1"
                     /translation="MRFHCWFTALLGAGSVLTADAAAQASPLSPDTRSFVSIDAPALV
                     LLHARVIDGTGGQPRENLVLVVERGLIRAMGDSAQVAVPSGARVVDLSGRTVLPGFVM
                     VHEHMFYPGVLWRSGYLANEQGFSFPRLYLAGGATTVRTGGSLEPYTDLELRAAIDSG
                     RIPGPKMDVTGPYLQGPGAPLFYQMHVLSGPDDAKRTVEYWLGQGVTSFKAYTNITRD
                     ELGAAIRAAHAKGIKVTGHLCSVTFHEAAALGIDNVEHGLLVASDFVADKKPDQCPDD
                     SAVESSLLALDMNGEKLQALIRDLVAHKVAITSTLTVFETFGPSRPPLPDRVLDVMTA
                     DARIAYLRQRARIADGGDTTWTVLLKKEMAFERAFVAAGGLLLAGTDPTGYGGVVAGF
                     SNQCEVELLVEAGFSPLEAIRIATLNGARYLGRDKQVGSLAPGKQADLIVVRGDPSHN
                     IKDIENVETVFKDGVGYDPAKLIASARGMVGLN"
BASE COUNT          619 a         1026 c         1026 g          552 t
ORIGIN      
        1 cgagattgct cacgtagatc ttgtccactc cgatctcccg tagagcacag atcgttttcg
       61 cgacgatcgc ctccggtgct aggccagcct cgaaatcccg cgtcacccct tctaccggca
      121 ccggaaagaa gtcgcccagc tgcctgagcg tccgcggatt cgcgctccgg tacaggaaga
      181 ctccgaacac cccggggaat ggcactccgc gccgccgcga ctctgccaga aagcgctcga
      241 cctgatcgag gtgatggtga gaaacgatct gagtcacgta gtagtcggcc tcgaagtccc
      301 ggtccagcaa gaagtccacc tgacgctcgg cgtcgcgatg aggattgatc cagccgccca
      361 ggttcagccg agggacccaa ccccgcatca tgcgccggag ctggtaggcg tgcggaacta
      421 ttcgcgtagt tccggtcgcc tgatccccgc cgacgacggt cagcgcctcg aacccgtgcg
      481 acgccgcgcg ggcggcatag agctggatgt attcgaggga gtgcttgcag gtcagaaatg
      541 gcaccagccg ttcagctggc agctcgccgg ccaggttggc cgtgagatgg gcgaggttct
      601 cctcctccga ccgccccacc gcgttgtcgg tcaggaaaat cagcgtgtcc tgccgagcca
      661 gccgccggat cgagtggtac atgtcgatcc aggcatcgac gctttcggcg tatgacatcc
      721 cactgcgtgg ggggcgcagc tcgactgcca ccaggggccg gtcttcccgg agccgcttga
      781 ggactgaggt gccggaagcg cttgaattgt ccagggtcat cgtggggggg aaacgtaaca
      841 ggtcggagca ttcccggttc gggcctgccg atgtgcgctg gaacgctgcg gagggacgtc
      901 ccctaccgta gcttgagtaa caagcgaatc ctcgaacgcc ctgagctgca acgatgacgc
      961 cgcaatggat ggcggtgttg atgctgacgg ccgcctgcgc cacagtcgga aaactcggag
     1021 cgccggctac accctaccga accgatccgc cccgagtcgg cgagatcccc gacccggagc
     1081 ttatcgacgt aattcggaag ggcgatctca tcgtcctggg cacgccggtc gaccgggttt
     1141 ccgaagccgg gttctacacg ccaaagttcc aaatgggagc gcgcgagacc tggtacagcg
     1201 tgaagatcgc ggtggactcg gtcgtaaagg ggaagctccg cagcgccaag aaggtcgacc
     1261 tgggaatgct gcccgtctgg atgaaccccg gcccgccgtt tcccagcctc gaagccaacg
     1321 aaatcgtggt gcagtatccc gagaccgagt cgcccaacat gcactgggga ctcgcaccgg
     1381 tcctcactct gggtgaacgg gcggtcttca tcttcaagaa gtgctggtac tgccttccgc
     1441 tcacgggagt ggccaatcct cgcggcccct actacgtggc gaacccgtgg gtggcgatgt
     1501 cgtggggaag taagctcccg cccgacgaat ggtcccgagt ggttcggttg acggagtcgc
     1561 tccgatcgcc acgcgcttcc cgttgaggcc tcaattgagg ccgaccatgc cccgagcgga
     1621 tgcgatcagc ttcgcgggat cgtagccgac gccatccttg aaaacggtct cgacgttctc
     1681 gatgtccttg atgttgtgcg agggatcgcc gcggacgacg atcagatctg cctgcttgcc
     1741 aggagccagc gatccgacct gcttgtcgcg gcccagatag cgggcgccgt tcagcgtcgc
     1801 aatccgaatc gcctcgagcg gggaaaaccc ggcttctacc agcagctcga cctcgcactg
     1861 gttggagaag cctgcgacca cgccaccgta gccggtagga tccgttcccg cgagaagcag
     1921 cccgccggct gccacaaaag cccgctcgaa ggccatttcc ttcttcagga gcaccgtcca
     1981 ggttgtgtct cctccgtccg caatgcgagc ccgctgccgc aaataggcga ttctggcatc
     2041 cgcagtcatg acatcgagca cgcgatctgg gagcggagga cgggatgggc cgaacgtctc
     2101 gaacacggtg agggtggacg taatcgcgac cttgtgggcg accagatctc ggatcagcgc
     2161 ctgcagcttc tcgccgttca tatcgagcgc gaggagcgac gactcgaccg cggagtcgtc
     2221 gggacactga tcaggctttt tatcggctac gaaatcgctt gcgaccagca gtccatgctc
     2281 cacgttgtcg atgccgaggg cggcagcttc gtgaaaggtg accgagcaaa ggtggcccgt
     2341 caccttgatg cccttggcgt gggcagcgcg aatggccgcg cccagctcgt cccgggtgat
     2401 attggtgtac gccttgaacg aggtcacacc ctgcccgagc cagtattcga cggtgcgttt
     2461 ggcgtcgtcc gggccggaga ggacgtgcat ctggtaaaag aggggagccc cgggaccctg
     2521 cagatacgga ccggtcacat ccatcttggg gccggggatc cggcccgagt cgatggcggc
     2581 cctgagctcg aggtcggtat agggctcgag actcccaccg gttctcaccg tggttgcgcc
     2641 gccggcgaga tagaggcgag gaaaactgaa gccctgctca ttggcgagat accccgagcg
     2701 ccacaagacg ccggggtaga acatgtgctc gtgcaccatg acgaagccgg gcagcacagt
     2761 ccggcccgag aggtcgacta ctcgagcccc ggacggaacc gccacctgcg cggagtcacc
     2821 cattgcgcga atgaggcccc gctcgaccac cagcaccaga ttttcccgcg gctgtccgcc
     2881 ggttccatcg atcacccggg cgtgcagtaa caccagcgct ggcgcatcga tggaaacgaa
     2941 gcttctggtg tcgggagaca ggggcgacgc ctgcgccgca gcgtccgccg tgagcacaga
     3001 gcccgcaccg agcagagcag tgaaccagca atggaagcgc atgtcgaggc ggagattacc
     3061 gtccctgggc gtacctgtca aaagagcatt gaaccggttt cgtggggccg atatcttccc
     3121 gcaccagcga ccgcagccac gagcctacta tgaccgagac catgaccgcc gttcctccgc
     3181 tgaccgttct gacggaggaa gaggaactgt tcagaagcac cgt
//
LOCUS       DASRJQ010000444         2059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1699990, whole genome shotgun sequence.
ACCESSION   DASRJQ010000444 DASRJQ010000000
VERSION     DASRJQ010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1699990"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VFB89_10040"
     CDS             complement(<1..511)
                     /locus_tag="VFB89_10040"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZM27687.1"
                     /translation="MAFVADPRSRTLPKVGADAPKRYQVSIVGAGLSGMYQLHRLRQL
                     GVSARVFEAGDDVGGTWYWNRYPGARFDSESWTYGFSFSDELLQEWDWSEHFSAQPET
                     LRYCNYVADKLDLRRDITFNSRAVSAAYDEYTNEWELAFADGRRVRSHFLITAIGPLS
                     APTMPAIPGV"
     gene            921..1292
                     /locus_tag="VFB89_10045"
     CDS             921..1292
                     /locus_tag="VFB89_10045"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZM27688.1"
                     /translation="MTITVKDLEAFADGWNRHDVDFLMTFMAEDCVFETSAGPEVSGK
                     RYVGREQVREGFAGVFRRFPDVHFGNARHFVSGDRGLSEWTFTATSTDGKKIEVTGCD
                     VFTFKGDKIAVKNSYLKNRTA"
     gene            1511..>2059
                     /locus_tag="VFB89_10050"
     CDS             1511..>2059
                     /locus_tag="VFB89_10050"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZM27689.1"
                     /translation="MAPEYAFLVTGAASGIGHATAVRLIERGHRVLSLDVKTPTAKVA
                     SHTSCDLSDPASIDAALLRIEEPISALLNIAGVPGTVGAEKTMRVNLLGLRHLTEALW
                     DRIADNGRIVNVASIAGNNWRKRRSALAELLSTSDFTAGLAWWQVKGAGVGTDAYTFS
                     KEAVVLYTMQLAGRGLTRGIRVN"
BASE COUNT          411 a          621 c          665 g          362 t
ORIGIN      
        1 ccacgcccgg gatggccggc atggtgggcg ccgacagcgg accgatggcg gtgatcagga
       61 agtgcgaccg gacacgccgg ccgtctgcga atgcgagctc ccactcgttg gtatactcgt
      121 cgtatgccgc cgacacggcc cgactgttga acgtgatgtc gcggcgcagg tccaacttgt
      181 cggcgacgta gttacagtag cgaagggtct ccggctgggc cgagaaatgc tcgctccaat
      241 cccactcctg cagcagctcg tcggagaagg aaaagccgta ggtccagctt tcggagtcga
      301 agcgcgcgcc aggatagcgg ttccagtacc aggtgccgcc gacgtcgtca ccggcttcga
      361 aaacgcgcgc agaaactccg agctggcgca atcggtggag ctggtacatg cccgagaggc
      421 cggcaccaac gatgctgacc tggtaacgct tcggtgcatc agctcccacc ttcggcaggg
      481 tccgagatcg cgggtctgca acaaacgcca tggcaagacc tattccagtg aacggaggag
      541 cgcaggagac gcgcggcggt ccaagcgcag aaccttggcc cgggcccggc tggagttcaa
      601 gccatgcgga cacaaagcgc accggctccg agggccggca gccacaacct cttgctccca
      661 catgggataa tcgaagggat cacaagacta cgagatacca cgagcagccg gagaccgctg
      721 cagtgcggtg ctatagtgct tcgatcgacg atcgcaggcg cgtgagaatg ggtgtcggtg
      781 ccgcccctgg cacttcgcca agaagtgccc ggtctggaat tggtcgcgcc cgacttgcaa
      841 gagtaggcga cgtatacggg aatccgctcg gacgggaact tgcacacgga gcgcaacctg
      901 gtgtgcgcac ggaggacacg atgacgatta ccgtcaagga cctggaagcg ttcgcggatg
      961 gctggaatcg ccacgacgtc gactttctca tgaccttcat ggcagaggac tgcgttttcg
     1021 aaacaagcgc gggaccggag gtctccggca agcgctacgt ggggcgtgag caggtgcggg
     1081 aaggtttcgc cggcgtgttc aggcggttcc ccgacgtaca tttcggtaac gcaagacact
     1141 ttgtgtcggg tgatcgcggc ttgtcggagt ggaccttcac ggccacgagc accgacggca
     1201 agaagatcga ggtcacgggc tgcgacgtct tcaccttcaa gggcgacaag attgcagtga
     1261 agaactcata cctcaagaac cgaacggctt agcaggtcat cctggccggc gagcttgatc
     1321 tgcatcgccg ccacctggac gaagagagct caggtgacga atacccgcaa agcgcagccg
     1381 accccattga agtggatccg tcacgctggc gcagcgggcg gcgacacagg tgcgagtgca
     1441 ttgcctgcgt tgagcacttg tcctagcgtt ccggcagtac tgtggagcag cgcaatcctg
     1501 agaggtcgat atggcacctg agtatgcgtt cctagttacg ggcgccgcat caggtattgg
     1561 ccatgccacc gcggtccggt tgatcgagcg cgggcaccga gtgctctccc tcgacgtcaa
     1621 gacgcccacc gccaaggtcg cctcgcacac gagttgtgat ctgtcggacc ccgcttctat
     1681 cgacgccgcc cttttgcgga tcgaggagcc catctccgct ctgctcaata tcgccggcgt
     1741 gccgggcacg gtcggcgcag agaagacgat gcgcgtcaat ctgctgggct tgcggcatct
     1801 gaccgaagcc ctgtgggatc gaatcgctga caacggccgg atcgtcaatg tcgccagcat
     1861 cgccggaaac aactggagga agcggcgcag cgctctggct gagttgctct cgacttctga
     1921 ttttactgct ggcctcgcct ggtggcaggt caagggcgcc ggcgtaggca ccgacgctta
     1981 taccttctcc aaggaggcgg tcgttctcta cactatgcag cttgcgggtc gcgggctcac
     2041 ccgcggtatt cgcgtcaac
//
LOCUS       DASRJQ010000445         3564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_300543, whole genome shotgun sequence.
ACCESSION   DASRJQ010000445 DASRJQ010000000
VERSION     DASRJQ010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300543"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..597)
                     /locus_tag="VFB89_10055"
     CDS             complement(166..597)
                     /locus_tag="VFB89_10055"
                     /inference="COORDINATES: protein motif:HMM:NF016330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HZM27690.1"
                     /translation="MAVHSIAVPPDPARDARARALVDQVPTWPKHTLKVAWGSFEAGT
                     VFRRATGSNVARYLVNAVACECPDYQQAGNICKHVRAVVLFEQRQQPASRYRPSYADL
                     FEGCRDCGDLADGLDGRCSKCASDREHRQRMARKRELVANL"
     gene            774..950
                     /locus_tag="VFB89_10060"
     CDS             774..950
                     /locus_tag="VFB89_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27691.1"
                     /translation="MRTKLVSVNLRLEPEMHAELVRIARDEERSLAGQIKLYLKRAIE
                     ERHAEHKAAREPER"
     gene            947..1453
                     /locus_tag="VFB89_10065"
     CDS             947..1453
                     /locus_tag="VFB89_10065"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HZM27692.1"
                     /translation="MTAGGSPITPLTQYQQRVWERTSKRPPWECCPWQGGISPDGYGQ
                     INTGGGMRPAHCVVWEWTHGPIPDGLQLDHTCHNAASACPGGKSCLHRRCVNPAHLEP
                     VTPLENQLRGMATRGRLDTCPNGHNYAVVGVYVGSPTSKHPGKRQCRACGRAQAERYR
                     ARKREAAA"
     gene            1529..1861
                     /locus_tag="VFB89_10070"
     CDS             1529..1861
                     /locus_tag="VFB89_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27693.1"
                     /translation="MAGPFVKLASIEEHPAGEEVWVNASTIAYIAPEGKDPEAARIFF
                     VVPNYESGGYALSLTVRGTPGAIVAAIGQAEMDDARRMASATGSSAVTGGQGAPMTMQ
                     TDVEGGES"
     gene            1960..2193
                     /locus_tag="VFB89_10075"
     CDS             1960..2193
                     /locus_tag="VFB89_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27694.1"
                     /translation="MATESWHPKRGDHVRIRATGIEATAMKFDGHRVRVHYDQITIAP
                     GPEEVKADFLTPVHRVPQRPPAWFDLDELEPAE"
     gene            2217..2753
                     /locus_tag="VFB89_10080"
     CDS             2217..2753
                     /locus_tag="VFB89_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27695.1"
                     /translation="MTDHEPSAEPLANVSTDLTALADARARVRIDATERDTDTVGFVL
                     GQYRTQTGRTERTLADWLGISLPVLADLAEEVRPEKRRLLAGHSGDRPRPARRGVRRR
                     PGAAPRSLRPRRPVTETGGATCEQVRPGESPGARLAAYSFLSAHYMYEAWAESISGDM
                     LSVGEHGEEPGEFSYGAP"
     gene            2740..3132
                     /locus_tag="VFB89_10085"
     CDS             2740..3132
                     /locus_tag="VFB89_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27696.1"
                     /translation="MAHPEVKRAWAVAQRLGGSVSRLVRRATGDRRPLGGMASAPQAA
                     TLSQPVIPLTSLRRSLGRAAVACENQNVCGGVIELALYEHSASSSDVGETSVTEATML
                     QQTCECDLSEEERERVVDRVQQAIRMRG"
     gene            3237..>3564
                     /locus_tag="VFB89_10090"
     CDS             3237..>3564
                     /locus_tag="VFB89_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27697.1"
                     /translation="MAIRAEHPDEKKPKRQNGEGSVFFWPGRGWYAAVTGGDGRRIMR
                     KAPKQTERGAEMLLRELLAQRRAGELTRGRTTLAEFKDEWLRSCKRRNCRPGTLDTYE
                     KKLSSYV"
BASE COUNT          669 a         1116 c         1261 g          518 t
ORIGIN      
        1 agcgtggtcg tgcgccggtc cggctccggg gcgtcgcgct gcccctcagc gcgggcgtgc
       61 tcggcggcaa tccggtcgaa caggtcgaac gtagcgttca tggcggggct cctcagcggt
      121 aagggggccg gcgccaaccc cggttggcgc cgaccccagc ggcgatcaga ggttggcgac
      181 cagctcgcgc ttgcgagcca tccgctgccg gtgctcccga tcgctggcgc acttcgagca
      241 gcgaccgtca aggccatccg ccaggtcgcc gcagtcgcgg cagccctcga acaggtccgc
      301 gtaggacggg cggtaccgag aggccggctg ctggcgctgc tcgaagagga cgacggccct
      361 gacgtgctta cagatgttcc cggcctgctg gtagtcgggg cactcgcagg cgaccgcgtt
      421 gacgaggtag cgggcgacgt tgctgccggt cgcccggcgg aacacggtgc cggcctcgaa
      481 gctaccccac gccaccttga gcgtgtgctt cggccaggtc ggcacctggt cgacgagggc
      541 acgggcgcgg gcgtcgcgcg ccgggtcggg cgggacggcg atactgtgaa cggccatctg
      601 ggtgtacccc cttttggtct ggccccgcgg gtggtacgaa cactcgcggg gcttttgcgt
      661 ggtcgggcac ggacgcctga cctacggaaa gtttaccaca agacggcacc ataatgctat
      721 aatatggtgc cacttcgggc ggatggcgcg gcatcgtggc tacactgcgc gctatgagga
      781 cgaaactcgt gtccgtgaac ctgcggctcg aaccggagat gcacgccgaa ttggtgcgca
      841 tcgccaggga tgaggagcga tcgttggccg gccagatcaa gctgtacctg aagcgggcga
      901 tcgaggagcg ccacgccgag cacaaggctg cgcgggagcc ggagcgatga cggccggcgg
      961 atcgcccatc acaccgctaa cgcagtacca gcagcgcgtc tgggaacgaa ccagcaagcg
     1021 cccaccgtgg gaatgctgcc cctggcaagg tggcatcagc ccagatgggt acggacagat
     1081 caacaccggc ggcgggatgc ggccggcaca ctgcgtggtg tgggagtgga cgcacgggcc
     1141 gatccctgat ggcctccagc tcgaccacac ctgccacaac gcggcgagcg cgtgcccagg
     1201 tgggaaatcg tgcctacacc gccgctgcgt caaccccgct caccttgagc ctgtaacgcc
     1261 gctggagaac cagttacgtg ggatggcgac gcgtgggcga ctggacacct gtccgaacgg
     1321 ccacaactac gccgtggtgg gggtgtacgt tggcagccca accagcaagc accccgggaa
     1381 gcgacagtgc cgagcatgcg ggcgtgctca ggccgaacgg tatcgggcca ggaagcgtga
     1441 agcagcggcc tgagctagca ggccaccagg tgccggcaac tacctgtgga gagagcggcc
     1501 ggcaaagaca gtgtaacggg agccgaccat ggcagggcca ttcgtgaagc tcgcatcgat
     1561 cgaggaacac ccagccggcg aagaggtctg ggtgaacgcg agtaccatcg cctacatcgc
     1621 gccagaggga aaggacccgg aagcggcgcg gatcttcttc gtggtcccca actacgagag
     1681 tggtggctat gcgctgtccc tcacggtccg tgggacgccc ggtgcgatcg tcgccgcgat
     1741 cggtcaggcc gagatggatg acgcccgtcg catggcctcg gccaccggca gttcggcggt
     1801 tacagggggg cagggcgcgc cgatgacgat gcagacggat gtcgaggggg gcgagagctg
     1861 aacgacacga aaaagcggcg gacttcaagc tcaggccacc gcctcagaga cgccggccct
     1921 caccagagca agcgacggcc ggcggagagc gtatccatca tggcaaccga gagctggcac
     1981 ccgaagcgcg gcgaccacgt ccgaatccgg gcgaccggga tcgaggccac cgccatgaag
     2041 ttcgatggcc accgcgtcag ggtccactat gatcagatca cgatcgcgcc aggcccggag
     2101 gaggtcaagg ccgacttcct gacccccgta caccgagtgc cgcagcgtcc acccgcatgg
     2161 ttcgatctgg acgagctcga gccggcggag tgaagcagca ccctcaagag gcggccatga
     2221 ccgatcacga acccagcgcc gagccgctcg ccaacgtcag caccgacctc accgccctgg
     2281 ctgatgctcg ggcacgggtc cgcatcgacg ccaccgaacg cgacaccgat acggtcggct
     2341 tcgtgctcgg tcagtaccgt acccaaaccg ggcggacgga gcggacgctg gccgactggc
     2401 tcgggatcag cctgccggtg ctggccgacc tggcggaaga ggtccggccg gagaaacgac
     2461 ggctcctcgc tggccactca ggagataggc ctcgaccagc tcgccgaggt gtacggcgcc
     2521 gaccgggtgc ggctcctcga agccttcgac cgaggcgacc cgtgactgag accggcgggg
     2581 ccacatgcga gcaggtgcgt ccaggtgaga gtcccggggc gaggttggcg gcctattcat
     2641 ttctttctgc ccactatatg tatgaggctt gggcagaaag catttcaggg gacatgctga
     2701 gcgtgggtga gcatggcgag gagccagggg agttttcgta tggcgcaccc tgaggtcaag
     2761 agagcctggg ccgtagcgca gcgcctcggc ggctcagtga gccgcctggt gcgacgtgcg
     2821 acaggcgatc gacggcccct cggcggcatg gcgtcagcac ctcaggccgc tacgctgagc
     2881 cagcccgtca tccccctgac cagcctcagg agatcgctcg ggcgggccgc tgttgcctgt
     2941 gagaatcaga acgtgtgcgg aggcgtcatc gagctggcgc tgtacgagca cagtgcctcc
     3001 agcagtgacg tgggtgagac ctccgtgacc gaggcgacca tgcttcagca gacgtgcgaa
     3061 tgcgatctct ccgaggagga acgcgagcgg gtcgtggacc gggtccaaca ggccatcagg
     3121 atgcgcggat gaccgtcaaa ctggactggg tacggcccgg ccaattcatt ctgccctatt
     3181 agtaatagag gcttgggcag aaagcatttc cgggatcatg cgaggtgtga ggtagcatgg
     3241 cgatacgtgc tgagcatccc gacgagaaaa agcccaagcg ccagaacggc gaggggtcag
     3301 tgttcttctg gcccggccga ggctggtacg cggcggtaac cgggggcgac ggtcggcgca
     3361 tcatgcgcaa ggcaccgaag cagacagagc gtggtgccga gatgttgctc cgcgaactgc
     3421 ttgctcagcg tcgggcgggc gaactgacgc gggggaggac gacgctcgcc gagttcaagg
     3481 acgagtggct ccggtcctgc aagcgtcgga actgccggcc tgggacgctc gacacctatg
     3541 aaaagaagct gtcgtcgtac gtct
//
LOCUS       DASRJQ010000446         2440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2047172, whole genome shotgun sequence.
ACCESSION   DASRJQ010000446 DASRJQ010000000
VERSION     DASRJQ010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2047172"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VFB89_10095"
     CDS             <1..360
                     /locus_tag="VFB89_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27698.1"
                     /translation="PLPRPALRLELALSALRAVEGELGRLIEVVAAEMGLPAFLVVRK
                     PVCTALGTAVDMARSDVSGNEPGAAQEPYMRRIYLSEAGRRGSPPGTGGYPSPAGFWG
                     VVARLALLLHAVAVLYT"
     gene            complement(769..1050)
                     /locus_tag="VFB89_10100"
     CDS             complement(769..1050)
                     /locus_tag="VFB89_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27699.1"
                     /translation="MAVAVVYRPPALDAEKYKASWADGSPMPDPEGLLFHAGVGEGDD
                     FFTVSVWESREAYDAFGSRFKEALGAEGYEGGAPKILPVHHTIDHGVPA"
     gene            2033..2263
                     /locus_tag="VFB89_10105"
     CDS             2033..2263
                     /locus_tag="VFB89_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27700.1"
                     /translation="MSVGPSHAAGSPSPERELPGEFVSDDLRNDCARFLGELRQLVEL
                     RRELTPDSAAWAPSRLGDPEAAPNSRSAQSRQ"
BASE COUNT          438 a          799 c          791 g          412 t
ORIGIN      
        1 cccctgccgc gtccggcgtt acgacttgaa ctcgcgctca gcgcactccg ggcagtagaa
       61 ggcgagctcg gccggctcat cgaggtcgtc gctgccgaga tgggcctgcc agcgttcctc
      121 gtcgtccgaa agccagtatg caccgcactc ggcacagcag tggatatggc cagatcggac
      181 gtttccggaa acgaacccgg agcagcgcaa gaaccgtaca tgagaaggat ctacctaagt
      241 gaagccgggc gacgggggag cccaccgggg actggcggct atccgtcccc agcaggtttc
      301 tggggtgtag tcgcccggct tgctctactt ctgcacgctg ttgccgtcct ctacacctag
      361 tcgcgggaga agcgccacgg tctcgtcgac cttgatggtg caggtccgtc aggcggcctc
      421 aacaacgagc accggcggct ggtccgcatc gtcgtctcgc ttcccgatca ctcgcagcgc
      481 ccgatggccg agcgggacgg tctagactcg agaggccttc cggccccagg gaggaggagc
      541 ctcgacctcg cagggccgct cgttcgggat cttcaagaag gcgctccagc gcaggaacgt
      601 ggtcgcggcc gcggctgcgg cgagagagct tccgcagctg aacctgctcg acgcgctcga
      661 gccaaccatg ctgatcgctc gcaaagactc gggccgtttc cccgcgggtc gcggcccgcc
      721 ggaacaaccc gaggcgacga tcgaaatatg cttgaacctc ggatcgagct aggcgggaac
      781 cccgtggtcg atcgtgtgat gcaccgggag tatcttgggc gctcccccct cataaccttc
      841 tgcccccaac gcctccttga accgcgatcc gaaggcatcg tatgcctcac ggctctccca
      901 cacggagacg gtgaagaagt catcaccctc acctaccccg gcgtggaaca gaaggccctc
      961 agggtcgggc attggtgacc catcggccca gcttgccttg tacttttcag cgtccaacgc
     1021 gggcgggcgg tatacgacag ctactgccat cactacctcc tagttggcac gggtttggag
     1081 cgaggtgttt ggctggccta gtcagccggt acctctgaac gctttccagg ggagagagtg
     1141 cgaacctcac tccacccgcg ctatcccagt agtaccacgc cacgcgcagg ccgtcatcgg
     1201 taggaactac tcgggatggt ggcatcccat gagtcaccac gcattacaac caatcgaaaa
     1261 ggaacgtggc ggaatgagcc actagcgcag cagctgtgag gccgtaggtt cgctaagggc
     1321 cgggtcaagc ggaaggcggg atacgcctgc ggacactgaa gacgtggccc gcgcctttcc
     1381 ccgcgagcga ggcaacctgg cgctcagacg gcctctatcc gcgtgaaggg cgaggtgggc
     1441 tacgcgaatg cgagcgtacg tgggcggcga gtcggataag agccgagcga ccgcagtgcc
     1501 tctcccccta tgccctccgc tcggctccga ccgcagatgt tgcccgctgg cggcgcggcc
     1561 cacacggcgc ccgaggctgt gataggtgga gccggagcgt ggcgtccgtt ccccctgcgg
     1621 ctcgggaacg tgcccctcat ggggggagag atgaggtgct ccggctccat gtgccgcaga
     1681 cttgggggat ccctcgaaga cttgtcgcct acggtatcgc tcggcgaagc cccttccaag
     1741 gcccgcgcgg gttcagctcg gggtggtcgg cgccgcagag ccgaccaccc cacttagctc
     1801 agcgcccgac acacacggcg gcgccgtacg aacggcgaga cggagataaa cgtttgggaa
     1861 gggttggcag aagttcgcgc tctttccgag gttttttccg cacgtacccc attcgagtga
     1921 ctgcactccg gcccgagcgt cgtaaagtcg ccggaccgcc cgctcgaagc ccagcgtgcg
     1981 tctgcggatc tacaacagaa gttttccaca gcttttccac agcgaggtgg ggatgagtgt
     2041 tggaccgagc catgcggccg gatcgccttc gccagagcgg gagcttccgg gcgagttcgt
     2101 ctccgacgac ctgcgaaacg actgcgcccg cttcctgggc gagctgaggc aactggtcga
     2161 actccgccgt gagctgacgc cggatagcgc ggcatgggcg ccgagccgct tgggtgatcc
     2221 tgaggccgcg ccgaactccc gctcggcgca gtcccggcag tagaagacga gctcgggcgg
     2281 ctcatcgagg tcgtcgccaa cgtggtacgc ccgccaacgt tcgtcgtcgt ccgagagcca
     2341 ccgcctgtgc aggtcgtgat ggaagcgatc tcaggcaccg cgtttgaggc tacgacgtga
     2401 acgcgccatt gcctgtgacc aaacgggacg gtctagactc
//
LOCUS       DASRJQ010000447         5287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3695870, whole genome shotgun sequence.
ACCESSION   DASRJQ010000447 DASRJQ010000000
VERSION     DASRJQ010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3695870"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /gene="moaA"
                     /locus_tag="VFB89_10110"
     CDS             complement(<1..829)
                     /gene="moaA"
                     /locus_tag="VFB89_10110"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HZM27701.1"
                     /translation="MILSSALASQFDASHRLIDTFGRPLGSLRLSVTDRCNLRCRYCM
                     PEADYVWLPRDSILTFEEAERIVRVFTSLGVTKVRLTGGEPLLRHDLPELVKLLAANP
                     AVADLALTTNGLLLARHAQALRDAGLARITVSLDTLRPERMREFARSARHADVLEGLV
                     AARVAGFAGLKLNCVVIRGYNDDELEALIEFGRAYEAEVRFIEYMDVGGATQWSMDQV
                     VSQREILARLSARYGPVRPVMAPDAPAAPAERFALSDGTTFGIIASTTAPFCRSCDRS
                     "
     gene            complement(826..1320)
                     /locus_tag="VFB89_10115"
     CDS             complement(826..1320)
                     /locus_tag="VFB89_10115"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HZM27702.1"
                     /translation="MSFLRDEPLDLGRLVATVSAPERGAVVCFVGTVRDHHEGKSVLR
                     LEYSAYQPMAEAECGRIVAEARNRWDATVALEHRTGVLEAGETSVAIAAAAPHRTAAF
                     AACRYVIEEVKRRVPIWKREHYTDGTVGWVDPTQARRRAVSQAAKDAPAGPTDAAGQD
                     EWGV"
     gene            complement(1322..1588)
                     /locus_tag="VFB89_10120"
     CDS             complement(1322..1588)
                     /locus_tag="VFB89_10120"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HZM27703.1"
                     /translation="MTVSSNPTVTVRVLLFARYAEALGDSALTVTVSAPATLGSVLKA
                     IRSRPGGELLPERPLCAVNLIQARLDSPIQAGDEIAVLPPLAGG"
     gene            complement(1633..1971)
                     /locus_tag="VFB89_10125"
     CDS             complement(1633..1971)
                     /locus_tag="VFB89_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HZM27704.1"
                     /translation="MTKADLVEQVTASIARTAGPMISKKDCARVVDAFLEAVKDALKQ
                     QQNIEVRGFGTFKIRRRKTRMARNPRTGDPVEVAARPVPVFKPSKELRALVAEESPET
                     GEAVPGAPGA"
     gene            complement(2052..3167)
                     /gene="carA"
                     /locus_tag="VFB89_10130"
     CDS             complement(2052..3167)
                     /gene="carA"
                     /locus_tag="VFB89_10130"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HZM27705.1"
                     /translation="MTARPAFVLLEDGTWFPGTVPRPIEPAFGEVVFTTNITGYQETF
                     TDPSYLGQIVVMTAPMIGNYGVNAEDMESERPQVSGVVVRELARQPSNWRSDVSLDHW
                     LEQAAIPLIEGVDTRRLTRHIRERGAMRGVLAEGQEPGRELSALLMASRRMEGLDLAS
                     KATVHDRMTYGEGPHIVAYDYGMKRNIVRMLVATGCRVTVVPATTSAAEVRELGPDGL
                     FLSNGPGDPAAVSYTFDTIRALSGRGLPTFGICLGHQLIGIAFGGATAKLPYGHRGGN
                     HPVRDARTGRVLITTQNHGFAVVGDEGGVPGAPALEVTHLNLNDGTIEGIRHRELPLF
                     AVQYHPEAAPGPHDAYPHFAEFLSLVRAQAAAKSPTP"
     gene            complement(3196..3681)
                     /gene="secG"
                     /locus_tag="VFB89_10135"
     CDS             complement(3196..3681)
                     /gene="secG"
                     /locus_tag="VFB89_10135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HZM27706.1"
                     /translation="MFYLLLVLLILDALLLSVVVLLQAGQGGGLASLGGGNTDTVLGG
                     RQAVTILTKATWWCGGIFLALSLALQFVPRGGSGSAIQERLRASTPSAPVELPGAAPA
                     APAGGADSAAPAAEQPAAGQPAAPKSATPQPGTAPQPGTAPQAPAKKPAAAPPSPQPK
                     Q"
     gene            complement(3717..4475)
                     /gene="tpiA"
                     /locus_tag="VFB89_10140"
     CDS             complement(3717..4475)
                     /gene="tpiA"
                     /locus_tag="VFB89_10140"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HZM27707.1"
                     /translation="MSRHLTYAANWKMHHGPAAAREFAARFLELTNPVDKRSLWFFPP
                     AVSLAALVEAFRRRADIRVGAQNIHWEPKGAFTGELSLSLVSEVGATLTLVGHSERRH
                     LFGESDEQSARKVKAALAAGVVPLLCVGETLTEREAGRTEDAVRRQLEPVVAAVTEPE
                     ERRRVVIAYEPVWAIGTGKNATPRDAAEVHRFIRTWLSEQDWPGRVEVLYGGSVNARN
                     ALALLAEPDLDGVLVGGASLTPDGWAEIVGMGGG"
     gene            complement(4494..>5287)
                     /gene="pgk"
                     /locus_tag="VFB89_10145"
     CDS             complement(4494..>5287)
                     /gene="pgk"
                     /locus_tag="VFB89_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HZM27708.1"
                     /translation="RKFAELGDFYVNDAFGSAHRAHASTEAVAHLLKPAVSGFLMERE
                     LKYLGEALDRPARPFVAVLGGAKISGKIDLIDALLPKVDEIVIGGAMACTFFRAMGLE
                     TGNSLVEADRVDMAKALIDKAGRKLVLPSGAVVAQKLEPGVTIRTVKRDQIAPGWAMY
                     DIDRLTEQDFAAIIEAAKTVLWNGPMGVFETAPFDHGTLAVAHALARASAHGAVTVVG
                     GGDSAAAVAQAGLADKMTHVSTGGGASLEFLEGKVLPGVAALDEA"
BASE COUNT          918 a         1802 c         1693 g          874 t
ORIGIN      
        1 gactccggtc gcagcttcgg cagaacggcg ccgtggtgga tgcaatgatg ccgaaggtgg
       61 ttccgtcgct cagcgcaaac cgttcggccg gcgcagccgg cgcgtcaggc gccatcaccg
      121 gtcggacggg accgtagcga gccgacaggc gcgccaggat ctcgcgctgc gatacaacct
      181 ggtccatgct ccattgggtc gcgccaccta catccatgta ctcgatgaaa cggacctccg
      241 cctcgtaagc ccggccgaac tcgatcagcg cctccagctc gtcgtcgttg tagccgcgga
      301 ttaccacgca gttcagcttg agtccggcga atccggctac ccgggcggcg accagaccct
      361 cgagcacgtc ggcgtgccga gcgctgcggg caaactcgcg catccgctcg gggcgaaggg
      421 tatcgagact caccgtaatg cgagccagtc cggcatcacg cagcgcctgc gcgtgccggg
      481 ccagcaacaa gccgttggtg gtcagagcga ggtcggcgac ggcggggttg gccgcaagca
      541 gcttcaccag ctcgggcagg tcgtgcctca ggagcggctc gccgccggtc aggcgcacct
      601 tggtcacacc gagtgaagtg aacacccgga cgatccgctc ggcttcttcg aaggtcagga
      661 tcgaatcgcg cgggagccac acgtagtccg cttccggcat gcagtagcgg cagcgcagat
      721 tgcagcggtc agtcaccgag agtcggagac ttcccagcgg ccgcccgaag gtgtcgatca
      781 gcctgtgcga ggcgtcgaat tggctcgcga gcgcactcga aaggatcata cgccccactc
      841 gtcctgcccg gccgcgtcgg tcggtcccgc cggagcatct ttggccgcct gactcacggc
      901 gcgccgccgc gcctgcgtag ggtcgaccca acccaccgtg ccgtccgtat agtgctcccg
      961 cttccagatc ggcactcgcc gcttgacctc ctcgatgaca taccgacacg ctgcaaacgc
     1021 ggcggtccga tggggcgccg cagcagcgat cgcgacggac gtctctccgg cctcgagtac
     1081 gccggtgcga tgctcgagcg cgacggtcgc atcccaacga ttccgggcct ctgccacaat
     1141 gcgcccgcac tcggcttccg ccatcggctg gtacgccgaa tactcgagac gcaagaccga
     1201 tttcccctcg tggtgatcgc gcaccgtccc cacaaaacac acgacggcgc cccgttccgg
     1261 cgccgacact gtggcgacga gccgacccaa atccagcggc tcatcgcgca gaaaactcac
     1321 atcaccctcc agccagcggt ggcagcacgg cgatctcgtc gccggcctgg atcggggaat
     1381 ctagacgggc ctggatcaga ttgacggcac agagaggacg ctcaggaagg agctcaccgc
     1441 cgggtcgact ccgaattgcc ttgagcacgg atcccagtgt ggcgggagcg gaaaccgtca
     1501 cggtcaacgc ggagtcgccc aaggcttcgg cgtaacgggc gaacagcagc acgcggacgg
     1561 tgacggtcgg atttgacgag acggtcatgc gcttcaattg tcgccgaacg agcggccggg
     1621 cacggggtca gctcaggcgc ccggtgctcc cggaactgcc tcgccggtct cgggtgactc
     1681 ttccgctacc agcgcacgca actccttgct gggcttaaac accggcaccg gccgggcagc
     1741 gacttccacg gggtctccgg tccggggatt ccgagccatt cgggtcttcc gacggcggat
     1801 cttgaaggtg ccgaaccctc gtacctcaat gttttgctgc tgcttgagcg cgtcctttac
     1861 cgcttccagg aaagcgtcca ctacacgcgc gcagtccttc ttggaaatca tgggaccggc
     1921 ggtgcgggcg attgaagcgg tcacctgctc tacgagatcg gccttggtca tgcatccccc
     1981 aggagggtcg cggacgaggg ggcgccccga agggctcccg aggccgattg tacgggctta
     2041 agtgttgtct gtcaaggggt tggggatttg gcggctgcct gggcccgaac cagagagagg
     2101 aattccgcga agtggggata cgcgtcgtgg ggacccggcg cggcttcggg gtgatactgc
     2161 actgcaaaga gcggaagttc gcgatggcga atgccttcga tggtgccatc gttgaggttg
     2221 agatgcgtga cctcgagtgc cggcgctcca ggcacgccac cctcgtcgcc gacgacggcg
     2281 aagccatggt tctgtgtggt gatcaggacg cgtcccgttc tggcgtcacg caccggatga
     2341 ttgcccccgc gatggccata cggcagcttc gccgtcgcgc ccccgaaggc gatcccgatc
     2401 agctgatgtc ccaggcagat accgaatgtc gggaggccgc gaccgctcag cgctctgatc
     2461 gtgtcgaagg tgtagctcac ggcggctggg tcgcccgggc cgttgctgag aaacagaccg
     2521 tccggcccca gctcgcggac ttccgcggcc gacgtcgtcg caggaaccac agtcacccgg
     2581 caaccggtcg cgaccagcat ccgcacgatg ttccgcttca tcccgtagtc gtacgcgacg
     2641 atgtgcggcc cttcgccata agtcatgcga tcgtgcacgg ttgccttcga ggcgagatcg
     2701 agcccttcca ttcgacgcga agccatcagc agcgcgctca actcgcgccc tggctcctga
     2761 ccctcggcca gcacaccgcg catcgcaccg cgctcgcgga tgtgtcgcgt gaggcggcga
     2821 gtatccacac cttcgatcag cggaatggca gcctgctcga gccagtggtc gagtgacacg
     2881 tctgatcgcc agttcgaggg ttggcgggcc agctcgcgca ccacgacgcc acttacctgc
     2941 ggccgctccg actccatgtc ctcggcgttg acaccgtagt tgccgatcat cggagcagtc
     3001 atcactacga tctggcccag gtagctcgga tcggtaaagg tctcctgata gcccgtaatg
     3061 ttggtggtga ataccacttc accgaaggcg ggctcgatgg ggcgcgggac agtgcccggg
     3121 aaccaggtgc cgtcttcgag caagacgaac gcggggcggg ccgtcatcgg ctcaccccgc
     3181 gttgcctgca cgggatcact gcttgggctg gggcgaaggt ggcgctgcag ccggcttctt
     3241 agctggggcc tgcggagcgg tgccaggctg cggtgcggta cccggctgtg gcgtcgccga
     3301 ctttggagcg gccggctgtc ctgcagcggg ctgttctgcg gccggtgccg cggaatctgc
     3361 tccacctgct ggggcagctg gagccgcacc aggcagctcg accggcgccg agggagtgct
     3421 ggcgcgcaag cgctcctgga tcgcgcttcc cgagccgcct ctggggacga actgcagcgc
     3481 gagcgaaagc gcgaggaaga tgcccccgca ccaccaggta gccttggtca ggatggtcac
     3541 cgcctgacga ccgcccagca cggtgtccgt gttgccaccg ccaagtgagg cgagtcctcc
     3601 gccctggccg gcctgaagca gtacgaccac cgagagcaga agcgcgtcga gaatgagtag
     3661 aacgagcagg agataaaaca tggcgggcta taggctagcg gcgtggtgaa cgaaaatcaa
     3721 ccgccgccca ttcccacaat ctcggcccat ccgtcaggtg tgaggctcgc accgccgacc
     3781 agtacaccgt ccagatcggg ctcggccagg agtgcgagcg cattcctggc gttgacgctt
     3841 ccgccataga gcacctcgac ccgaccgggc cagtcctgtt cgctcagcca cgtgcggatg
     3901 aagcggtgca cctcggccgc gtctcgcggt gtcgcgttct tgccggtacc gatggcccag
     3961 accggctcgt acgcgatcac gactcgacgt cgttcttccg gctcggtaac agcggcgacc
     4021 accggctcca gctggcgacg taccgcgtct tccgtgcgcc cggcctctcg ctcggtgagc
     4081 gtttcgccca cgcagagcag cggcacaacg cccgcggcga gggcggcctt caccttgcgg
     4141 gcgctctgct cgtcgctctc gccgaataaa tgcctgcgct cggagtggcc caccaaggtc
     4201 agtgttgcgc cgacctcgct taccagcgaa agcgacagct cgccggtgaa tgcccccttc
     4261 ggctcccaat ggatattctg agcccccacc cggatgtccg cgcggcgacg aaacgcctcg
     4321 accagcgccg ccagcgacac cgccggcgga aagaaccaga gtgaccgctt gtcgaccggg
     4381 ttggtaagct ccagaaaccg cgcagcgaac tcccgagccg cggcgggccc gtgatgcatt
     4441 ttccagttgg cggcgtaggt gagatgacgg gacatggtca gacaaaacag cgttcacgct
     4501 tcatctaaag ccgccacccc cggcaacacc ttcccctcca gaaactccag gctcgcgccg
     4561 ccgcccgttg agacgtgagt catcttgtcg gctagtccgg cttgagccac cgcagccgcc
     4621 gagtcgccgc cgccaacgac ggtaacggcc ccgtgtgccg aggctcgcgc gagcgcgtgg
     4681 gcgacggcca gggtaccgtg atcgaacggc gcggtctcga acacgcccat cgggccgttc
     4741 cacagaacgg tcttcgctgc ctcgatgatc gccgcgaagt cctgctctgt caggcgatcg
     4801 atgtcgtaca tcgcccagcc cggcgcgatc tgatcacgct tgaccgtccg gatggttacg
     4861 cccggctcca gcttctgggc gaccacagcc ccgctcggca gtaccagttt tcttccggcc
     4921 ttgtcaatca aagccttggc catgtccacc cgatcagcct cgaccagcga gttccccgtc
     4981 tcgagcccca tggcgcggaa gaaggtgcac gccatcgcgc cgccgatcac gatctcgtcc
     5041 actttgggca ggagggcgtc gatcagatcg atcttgccgc tgatcttggc gccgccgagc
     5101 accgcgacaa atgggcgcgc ggggcgatcg agcgcttcgc ccagatactt gagctcacgc
     5161 tccatcagaa acccggacac cgccggcttc aacaagtgag cgacggcttc ggtgctggca
     5221 tgcgcccgat gcgccgagcc gaacgcgtca ttcacataga aatcgccgag ctccgcgaac
     5281 ttccttg
//
LOCUS       DASRJQ010000448         2314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2803901, whole genome shotgun sequence.
ACCESSION   DASRJQ010000448 DASRJQ010000000
VERSION     DASRJQ010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2803901"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1231
                     /gene="gatA"
                     /locus_tag="VFB89_10150"
     CDS             <1..1231
                     /gene="gatA"
                     /locus_tag="VFB89_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HZM27709.1"
                     /translation="AVKDNIVTIEEPTTCGSRILEGYLSPYTATAVRRLRAAGAMVAC
                     KANLDEFAMGSSTEHSAYGRVKNPLAHDRVPGGSSGGSAALVAADVVPAALGSETGGS
                     VRQPASFCGVVGVKPTYGRVSRYGLVAFGSSLDCISVFGKTVDEATRVLAVMSGPDPL
                     DATTVDRPPMKPAPAMASLQGLKVGLPREYFPTDLHPGVRAGLDRTVAVLRDLGATVS
                     EVSLPHSKYAVPTYYIIAPAEAAANLARYDGVRYGVRKVGPEGNIGALYRATRGSGFG
                     AEVRRRILIGTYVLSAGYYDAYYRKAQTVRALIAQDFQRVLGSNGVDLLLTPTTPTPA
                     FKAGEKTEDPVQMYLADIFVCAISLAGLPALSLPVGRDQGLPIGAQLIAAAFDEPRLL
                     AVAGLLERSLHGTEEVR"
     gene            1231..>2314
                     /gene="gatB"
                     /locus_tag="VFB89_10155"
     CDS             1231..>2314
                     /gene="gatB"
                     /locus_tag="VFB89_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HZM27710.1"
                     /translation="MSWETVIGLEVHVQLLTRSKMFCGCSTAFGDPPNTNVCPVCLGL
                     PGALPVPNARAIRLATRAALALGCTVNGRSVFARKNYFYPDLPKGYQISQFDQPLAIH
                     GGLEFESPERGRMRCGITRLHVEEDAGKSLHDRFPGKTAVDLNRAGVPLAEIVSEPDL
                     RSPAEARVYLTTLKQILMYAEVSDCSMEQGSLRVDANISIRKAGDSTLGTKSEVKNLN
                     SFANVERALTAERDRQIGLVESGQRITQSTLLFNAGTGQVRLLRSKEESHDYRYFPDP
                     DLPPLVLSKEWIEEQGAALPELPDAKRERLVMAYGLSLEHARVIAADRAFANYFEAVV
                     AAGAESQAAANWVMGDALAGYNAIGRF"
BASE COUNT          402 a          735 c          764 g          413 t
ORIGIN      
        1 cgctgtgaaa gacaacatcg ttaccataga agagccgacc acctgcggat cccgcattct
       61 cgaaggctat ctgtcgccct acaccgcgac tgcggtgcgg cgcctgcggg cggctggggc
      121 gatggtggcc tgtaaggcga atctggacga gttcgccatg ggatcgtcca ccgagcattc
      181 ggcctacggg cgggtcaaga atccactcgc tcacgatcgc gtccccgggg gatcctcggg
      241 cggatctgcg gccctggtag ctgccgatgt cgtgcccgca gcgctgggct cagagacagg
      301 cggttcggtg cgtcagcccg cgagcttctg cggcgttgtc ggcgtgaagc ccacctatgg
      361 gcgtgtcagc cgatacggcc tggtcgcgtt cggatcgtca ctcgactgca tctcggtgtt
      421 cggcaagaca gtggacgagg cgacccgggt attggcggtg atgagcggac cggatccgct
      481 ggacgctacg acggtcgatc gcccaccgat gaagcccgcg cccgcgatgg cgagcctcca
      541 gggtctcaag gtaggccttc cccgcgagta ctttccgacc gatttgcacc ccggcgtgcg
      601 tgctgggctc gatcggaccg ttgcagtgct ccgggatctc ggtgccaccg tcagcgaggt
      661 ctcgcttccg cattcgaagt acgccgtacc cacctattac atcattgccc cagctgaggc
      721 agcggcgaat ctggctcgct acgacggagt ccggtatggc gttcgcaaag ttgggccgga
      781 gggaaacatc ggggcgctct accgggccac gcggggctcc gggttcggag ccgaagtgcg
      841 ccggcgtatt ctgatcggca cgtatgtgct gagcgccggg tactacgacg cctactaccg
      901 gaaagcccag acggtccggg ctctgatcgc tcaggatttt cagcgggtgc tgggtagcaa
      961 cggcgtggat ctcctgctca cgcccaccac tccgacgccg gccttcaaag cgggcgagaa
     1021 gactgaagac ccggtgcaga tgtacctcgc cgacatcttc gtctgcgcaa tcagcctggc
     1081 gggccttcct gcgctcagcc tgccggtcgg ccgggatcag ggtctgccta tcggagcaca
     1141 gctcatcgcc gcagccttcg acgagccgcg cctgcttgct gttgccggat tgctcgagcg
     1201 atccctccac ggaacggagg aggtccgctg atgagctggg agaccgtgat tgggctcgag
     1261 gtgcatgtcc agcttctcac ccgaagcaag atgttctgcg gctgctccac ggcgttcggt
     1321 gatccgccca acaccaacgt gtgcccggtc tgcctcgggc tgccgggagc gctgccggtg
     1381 ccgaatgcgc gggcaatcag gctggccacc cgggcggcgc ttgcgctggg ctgtaccgtg
     1441 aatggtcgca gcgtgttcgc gcgcaagaac tatttctacc cggatctgcc caaagggtac
     1501 cagatctcac agtttgacca gccccttgcg attcacggtg gcctcgagtt cgagtcgccc
     1561 gagcgggggc gcatgcgatg cggcatcacc cgcctccacg tcgaggaaga cgccgggaaa
     1621 tcactgcacg atcgattccc tgggaagacg gcggtagatc tgaaccgagc gggggttcct
     1681 ctcgccgaga tcgtaagcga gcccgatttg cgatcgccag cggaggcccg ggtctatctc
     1741 acgacgctca agcagatcct gatgtatgcc gaggtcagcg actgcagcat ggagcaggga
     1801 agccttcggg tggacgccaa catttccatc cggaaggcgg gcgactccac gttaggcacc
     1861 aagtccgagg tcaagaatct caacagcttt gcgaacgtcg agcgcgctct taccgcggag
     1921 agggaccgcc agatcggcct ggtggagagc ggacagcgga tcacccagtc aacactgctg
     1981 ttcaacgcgg ggacgggaca ggttcggctg ctccgatcca aagaagaaag ccatgactac
     2041 cgctacttcc ccgaccccga tcttccgccc ctggttctgt cgaaggaatg gatcgaggaa
     2101 cagggggccg ctcttcccga gctgcccgac gccaagcgcg agcggctcgt catggcctac
     2161 gggctctcgc tcgagcacgc ccgggtaatc gcggccgacc gcgcatttgc gaactacttc
     2221 gaagcggtcg tcgctgccgg cgcggagtcg caggcggcgg ccaattgggt aatgggtgat
     2281 gcgctggccg gctacaacgc gatcgggcgg ttcg
//
LOCUS       DASRJQ010000449         4746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2695634, whole genome shotgun sequence.
ACCESSION   DASRJQ010000449 DASRJQ010000000
VERSION     DASRJQ010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2695634"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1439)
                     /gene="pckA"
                     /locus_tag="VFB89_10160"
     CDS             complement(<1..1439)
                     /gene="pckA"
                     /locus_tag="VFB89_10160"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HZM27711.1"
                     /translation="MIAPQRAVGDMTVQARFVQRETLTLEEHGLNPERILPNLTVPRL
                     IEYALRREEGVVTDHGAFAAVTTPKTGRSAKDKFVVVEPDSEAEIWWDKNARMDGRAF
                     DRLHDDVRAFLDTRELFVQDLFAGAEVAYRLSVRFITPSAWHALFVRHMFIRPQPADL
                     AQFQPGFTVLHAPEFQADPGRHGTRTGTFVAISLAKRLVLIGGTRYAGEMKKSIFTVL
                     NYLLPQQGVLPMHCSANVGPDHDTAIFFGLSGTGKTTLSADPSRSLIGDDEHGWSDTG
                     VFNFEGGCYAKVIRLRPDTEPEIFQATRTFGTVLENVVLDPATRAVDYDCDRITENTR
                     ACYPIHYISNHLPGGCGGHPVNVVFLTADAFGVMPPVARLTPDQAMYHFLSGYTAKVA
                     GTEAGVTEPTATFSACFGAPFLPLHPGVYAKMLGERIGGHGARVWLVNTGWTGGKYGT
                     GKRMPIKETRALLNAALDGSLKNVEFRKDP"
     gene            complement(1497..2390)
                     /locus_tag="VFB89_10165"
     CDS             complement(1497..2390)
                     /locus_tag="VFB89_10165"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HZM27712.1"
                     /translation="MHRSTVQPERLGAHVSIQGGVDTAPARGLAIGATAIQVFTKTPN
                     QWREPALSAGTCAAFRREVSRSGLRGVVAHDSYLINLASPDPLVSLRSIGAFKAELER
                     CTALGIRYLVSHPGNYIDDRDLGLKRNAEAYALCLQAVPGRVMVLLETTAGTGTALGA
                     NFEELAELRARIPAPLRRRIGFCADTCHLYASGYDLVRDFDGVWDYWDRVIGLRRLHC
                     IHLNDSKTALGARRDRHELIGEGTLGREPFRRLMTDPRFSRVIKILETPKGNDGNKMD
                     RKMLRRLRAYGKTARRVRSVA"
     gene            2390..3841
                     /locus_tag="VFB89_10170"
     CDS             2390..3841
                     /locus_tag="VFB89_10170"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZM27713.1"
                     /translation="MPLLETVTELLPENGGDRPRVLLVGDVSARPYGMERALSRAGFH
                     VIEAAAPAQGPPELAGKPDVAVITAGEADGSLAMLVQALQAAWGGQVPLIAVLGSPDR
                     EGPTRALGFGADDALASPLHLPELCARVETRARRRVGDRVAGNGRVQELLLDLMEHTW
                     IDRRLAEILEVMVDRVGRTLPRWEAAFVLATESVGAARIIAGSRGMASRDVRLDIERY
                     PELAEALRTARPVVIPDVQNDPLFEAARRRWGYEGTSVPVRAVAAMPMTIADRLVGVL
                     LLRTQDASARLSVTEELFAMQVARAAARLVDADARAPIGSNSSKATDALTGLPTTDVL
                     DRKIRDEAERAKRYALSFSLVLLDVDHQNALNDRLGRASGDRFLAELGRMFQAEVRAS
                     DFVARYGGDEFALVLAETGTYGARELVHRLRARLNVESFAGLPPGERPRIAAGIVAYP
                     HPAIEETDDLLVLLEAALRRGKAQAEERIGVAE"
     gene            complement(3872..>4746)
                     /locus_tag="VFB89_10175"
     CDS             complement(3872..>4746)
                     /locus_tag="VFB89_10175"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZM27714.1"
                     /translation="VLQRRLVEIYKRGPLYTFQALLAAESFGDLLSRYKYLYLTSRQD
                     RALVQDVEKLRNRVVVQRNDILRVRSELDRRRQEREAEYERYGELANERARRLAQLKR
                     SAQATEQKLSALERDEARLTDVLAALERARREAKVRAGAGLGPIAGPGSITTADIGKL
                     DWPVDGTIVYRFGRDTLPSGGVIRWNGIGIAAAAGTPVKAVEAGRVRLVGQFGTYGLT
                     ILLEHGNGYYSVYAHLQSASVKLNDAVNKAQVIGAVGGLSSDYGPHLHFEIRGENQIA
                     LDPADWLKKAAKQQ"
BASE COUNT          807 a         1512 c         1606 g          821 t
ORIGIN      
        1 gggtccttgc ggaactcgac attcttcagg ctgccgtcga gcgcggcatt cagcaacgcg
       61 cgcgtttcct tgatcggcat gcgctttcca gtgccatatt tgccgccggt ccagccggtg
      121 ttgaccagcc agactcgggc gccgtgaccc ccgatccgct cgcccaacat cttcgcgtag
      181 actcccggat gcagcgggag gaagggcgcg ccgaaacagg cgctgaacgt cgcggtcggc
      241 tcggtcacgc ccgcctccgt cccggctacc ttcgcagtat agccagacag gaaatgatac
      301 atcgcctggt cgggcgtgag ccgcgcaacc ggcggcataa cgccgaaggc atctgccgtg
      361 aggaacacga cgttgaccgg atgaccgccg caaccgcccg ggagatgatt gctgatgtag
      421 tggatcggat aacaggcgcg ggtgttctcg gtgattcgat cgcagtcgta atcaactgcc
      481 cgggtcgcgg gatcgagcac gacgttttcg aggacggtgc cgaacgttcg cgtcgcctgg
      541 aagatttcgg gctcagtatc gggccggagc ctgatgacct tggcgtaaca accgccctcg
      601 aagttgaaga cgcccgtgtc gctccagccg tgctcgtcgt cgccgataag gctgcgtgaa
      661 ggatcggccg agagcgtggt cttgccggtg cccgagagac cgaagaagat cgccgtgtcg
      721 tgatccggtc ccacgttggc ggagcagtgc atggggagca cgccctgctg cgggagcaga
      781 tagttgagca cggtaaagat cgacttcttc atctccccag cgtacctggt gccgccgatc
      841 aggacgaggc gcttggcgag actgatcgcg acgaaggtcc cggttcgggt gccgtgccgt
      901 ccgggatcgg cctggaactc cggcgcatga agcacggtga agccgggctg gaactgcgca
      961 aggtccgcgg gctgggggcg gatgaacatg tgccggacga acagcgcatg ccacgcactc
     1021 ggcgtgatga agcggactga aagccgatac gcgacctcgg ctccggcaaa gaggtcctgg
     1081 acgaataact cgcgggtgtc gagaaacgcc cgaacgtcgt cgtgcagtcg gtcgaaggcg
     1141 cgcccatcca tccgggcgtt cttgtcccac cagatctcgg cttccgagtc cggttccacc
     1201 accacgaatt tgtctttggc cgagcgccct gttttcggtg tggtcaccgc cgcaaaggcg
     1261 ccatggtcgg tgacgacgcc ttcttcccgc cggagcgcgt actcgatcag tctcgggacg
     1321 gtgagattcg ggaggatgcg ttcggggttg agcccgtgtt cttcgagggt aagggtttcc
     1381 cgctggacga atctcgcctg aaccgtcata tctcccactg cgcgctgagg cgcgatcaag
     1441 gtcacagcag cggcgcaggc gccgcagagt caggaaagat cgactcgcaa cgccgcctag
     1501 gcaaccgacc gaacccggcg ggcggttttg ccgtaggctc tgagccgccg cagcatcttg
     1561 cgatccatct tgttgccgtc gttacccttg ggagtttcga ggatcttgat cacgcgggag
     1621 aaacggggat cggtcatgag gcgccgaaaa ggctcgcgac ccagggtgcc ctccccgatc
     1681 agctcgtgcc ggtcacggcg cgcaccgagg gccgtttttg agtcgttgag gtgaatgcag
     1741 tgcaggcgcc gcagtccgat gacccggtcc cagtagtccc acacaccgtc gaagtcgcgg
     1801 accaggtcgt atccgctggc atacagatga caagtatcgg cgcagaaccc gattcgccgg
     1861 cggagcgggg cggggatccg agcgcgcagc tcagccagct cctcgaagtt ggccccgagc
     1921 gcggtgccgg ttccggccgt ggtctcgagc agaaccatga cccggcccgg gactgcctgc
     1981 aggcaaaggg cgtaggcctc agcgttgcgc ttcaggccta agtcacggtc atcaatgtag
     2041 ttgcccgggt gcgacaccag gtagcgaata cccagggccg tacagcgctc gagttcggcc
     2101 ttgaaagccc cgatggatcg cagacttacc agcggatccg gcgaggcgag attgataagg
     2161 tacgaatcgt gcgcgacgac accccgtaac cccgatctcg atacctcacg ccgaaacgcg
     2221 gcgcaggtcc cggccgagag ggccggctcc cgccactggt tcggcgtctt cgtgaaaacc
     2281 tggatcgcgg tagcgccgat ggcgaggccg cgcgccgggg ctgtgtccac gccaccctgg
     2341 atggagacgt gagcgcccag ccgttccggt tggacagtcg accgatgcat tgccactcct
     2401 cgagaccgtg accgagctcc tgccggaaaa tggcggggat cggccccgag tcctcctcgt
     2461 cggggacgtc tcggcgcgcc catacgggat ggagcgcgcg ctgagccgcg ccggctttca
     2521 tgtgatcgag gctgcggctc cggcgcaagg cccgcccgaa ctcgccggta agccggacgt
     2581 agccgtcatt acagccggcg aagcggacgg atcgctggcc atgctggtcc aagcgctcca
     2641 agctgcttgg ggcggccagg tccccctcat tgccgtgctc ggctcgcctg atcgcgaagg
     2701 gcccactcgc gcgctgggat tcggtgcgga cgacgcgctt gcgtcgccgc tccacctgcc
     2761 cgaactgtgc gcgcgtgtcg agactcgggc ccgtcgccga gtgggcgatc gtgtagccgg
     2821 caacggtcgc gtgcaggagc tgctgctcga tttgatggag cacacctgga tcgatcggcg
     2881 acttgctgag atcctcgagg tgatggtgga ccgggtaggg cgaacgctcc cgcgttggga
     2941 ggcggcgttc gtgctcgcga ccgagtcggt cggcgcggcc cggatcatag ccggcagccg
     3001 tggtatggcg tcgcgagatg ttcgactcga catcgagcgc taccctgagc tggccgaggc
     3061 gctgcgcacg gcaaggcccg tcgtcatccc ggatgtgcag aacgacccct tgttcgaggc
     3121 cgcccgcagg cgctgggggt atgaaggcac cagcgtcccg gtccgtgctg tcgctgccat
     3181 gccgatgacc atcgccgacc gcctggtcgg cgtgctgctc ctccgcacgc aggacgccag
     3241 cgctcgactc agcgttaccg aggagctgtt cgccatgcag gttgcgcgcg cggccgcccg
     3301 gctggtcgac gcggatgcgc gcgcaccgat cgggtccaac agttccaagg cgaccgacgc
     3361 gcttaccggt cttcccacca ccgatgtgct cgatcgaaag atccgggacg aggcggagcg
     3421 ggcgaagcgt tatgcgcttt cgttcagcct ggtgctgctg gacgtggatc accagaatgc
     3481 gctgaacgac cgtttaggcc gagcgagcgg cgaccggttc ctggccgagc tgggacggat
     3541 gtttcaggcg gaggtccggg cctcggattt cgtcgcgcgt tacggtggcg acgaattcgc
     3601 gctagtgctg gccgagaccg gcacgtacgg ggcaagagag ctggttcatc gattgcgagc
     3661 gcggctcaac gttgagtcgt tcgccggact cccgccgggc gagcgtccac gaatagcggc
     3721 gggcattgtg gcataccccc acccggcgat cgaggagacg gacgacctgc tggtcctgct
     3781 ggaggcggcg ctcaggcggg ggaaggcgca ggcagaggag aggattggcg tagcggaata
     3841 agaaggggga gcgggagaaa gggagagcgc cttactgctg tttcgccgct tttttcagcc
     3901 agtccgccgg atcgagcgcg atctggttct ctccccgaat ctcgaagtgc aggtggggtc
     3961 catagtccga gctcagtccg ccaaccgcgc cgatcacctg cgccttgttc accgcatcat
     4021 tcagcttgac cgacgccgac tggaggtggg catagaccga gtagtagccg ttgccgtgct
     4081 ccagcagaat ggtgagaccg taagtcccga actggcccac caaccgtacc cgacccgctt
     4141 cgaccgcctt gaccggtgtt cccgcggcgg cagcgatccc gatgccgttc cagcgaatca
     4201 ctccgccgct tggcagcgtg tcgcgcccga agcggtagac aatggtaccg tcgaccggcc
     4261 agtccaactt gccaatgtcg gccgtcgtga tcgagccggg acctgcaata ggtcccaggc
     4321 cggcgccagc gcggactttc gcttcgcggc gcgcgcgctc gagggcggcc agcacatcag
     4381 tcaaccgggc ctcgtcgcgc tccagcgctg acagcttctg ttcggtcgcc tgggcagaac
     4441 gcttgagctg ggcaaggcgc cgggcgcgct cgttggccag ctcaccgtat cgctcgtact
     4501 cagcttcgcg ctcttgccgc cggcggtcca gctcgctgcg gacgcgtagt atgtcgttcc
     4561 gctgcaccac gacgcgattc cgaagcttct cgacatcctg gaccaacgct cgatcctggc
     4621 ggctggtgag atagagatac ttgtagcggc tgagcagatc gccgaacgac tcggccgcga
     4681 gcagggcctg gaaggtgtac agcggtcccc gcttgtagat ctccaccagc cgccgttgca
     4741 gcacgg
//
LOCUS       DASRJQ010000450         2450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3454051, whole genome shotgun sequence.
ACCESSION   DASRJQ010000450 DASRJQ010000000
VERSION     DASRJQ010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3454051"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VFB89_10180"
     CDS             <1..504
                     /locus_tag="VFB89_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27715.1"
                     /translation="LVVPPSRELPPPQAKATPKNPAWPNDPDIARARKVEAKKKISTG
                     NASTALENQGRPISPDELNRGVTGSVPQSNRSNAPTPGTDPDGRQVAPSDLGYFGGLF
                     SGRAWGFAPYREETGTFTSEPPRSQLTEPPPGYQTPSAAQPYGVTKRIERTPVQQHDP
                     AGRDYGR"
     gene            599..1975
                     /locus_tag="VFB89_10185"
     CDS             599..1975
                     /locus_tag="VFB89_10185"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZM27716.1"
                     /translation="MRHAMKRAVVALATLSFSIFAGSIAHTANGWGPDVTHFTLANGL
                     EVVVIPDRRTPVITHMVWYRVGAADETPGKSGLAHFLEHLLFKGTKRNPQGLFSQTVA
                     TIGGQENAFTSSDYTGYYQRTSRERLKLMMEFESDRMTNLVLTDDVVKPELQVILEER
                     NSRVENNVNARLSEQMEAALYLNHPYGRPVIGWRHEMEGLTREDALEFYRRFYTPNNA
                     ILIIAGDVTPDEVKKLAEETYARVPRVAEVKPRVRPQEPRQEAPRTVTLSDPRVTQPS
                     LSRYYLVPSYSSGRPGEAESIDVLTHVLGRGANSRLYKKLVVDTGIAVSAGASYYGTA
                     LDSTRLGVYATPKPGTSLTQVEEAIDAVLTELIEKGVTQEELERSRNRLIADAVYAQD
                     SQSSLARWYGASLTTGLTIEQIRSWPDRLRAVTVDDVKNAAKQALDKRRSVTGYLVRQ
                     AGSRDDRS"
     gene            1990..>2450
                     /locus_tag="VFB89_10190"
     CDS             1990..>2450
                     /locus_tag="VFB89_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HZM27717.1"
                     /translation="MLLFARSAVARLGAMSLIVAALVALCVPASATKIERVVSPSGIE
                     FWFVRDATVPLVSIEFAFRGGAVQDPDDKMGLAEMTVSTLDEGAGDLDGTAFHARMER
                     NAIELQIRASREHVRGTLRTLKENQDEAFNLLRLALNEPRFESSAVERIRS"
BASE COUNT          474 a          835 c          756 g          385 t
ORIGIN      
        1 ctcgtcgtcc cgccgagccg cgagttgccg ccaccgcagg ccaaggcgac cccgaagaac
       61 cccgcttggc cgaacgatcc cgatatcgcg cgggccagga aggtagaggc caagaagaag
      121 atcagcaccg gcaacgccag caccgccctc gaaaatcagg gacggccgat ttcgcccgac
      181 gagctgaacc ggggtgtcac cggcagcgtg ccacaatcca accgctccaa tgcaccgact
      241 cccggcaccg atccggacgg gcgccaggtt gctccgtccg accttggcta ttttggcgga
      301 ttgttctctg ggcgggcctg gggcttcgcc ccttaccggg aggaaaccgg gactttcacc
      361 agcgagccgc cacggtcgca gctcaccgag cccccgcccg gctaccagac gccgtcggcg
      421 gctcaaccct atggggtgac caaacggatc gagcggaccc ccgtgcaaca gcacgatccg
      481 gccggccgtg attacggccg atagagccgc aattgggccg tattcggggg gctcttgcgg
      541 cggtattccc ggatcgccat ttgttgcccg ggcgagcgac caatccaaga ggcctccctt
      601 gcgccatgcg atgaagcgcg ccgttgttgc gctcgccacc ctgtccttct ccatatttgc
      661 gggctcaatt gcacacaccg ccaacggctg ggggcctgac gtcacccact tcacgctcgc
      721 caacgggctg gaagtggtcg tgatcccgga ccgccggact cccgtcatca cccacatggt
      781 ctggtatcgg gtcggcgccg cagatgagac accgggaaaa tccggtctcg cccatttcct
      841 cgagcatctg ctgttcaaag gcaccaagcg gaacccgcag ggcctgttct cgcagacagt
      901 ggccaccatc ggcggccagg agaacgcctt cacctcctca gactataccg gctactacca
      961 gcgaacgtcg cgcgagcggc tgaagctcat gatggagttc gagtccgacc gcatgaccaa
     1021 tctggtgctc accgacgacg tggtgaaacc ggaactgcag gtcattctcg aagagcgcaa
     1081 ctcgcgggtg gaaaataacg tcaatgcgcg gctctccgag cagatggaag ccgcactcta
     1141 cctgaaccat ccctatggcc ggccggtcat cggctggcgc cacgaaatgg aagggctcac
     1201 gcgcgaggat gcactcgaat tctatcgccg cttctacaca cccaacaacg ccatcctgat
     1261 catcgccggc gacgtgactc cggacgaggt gaagaagctc gcggaggaaa cctacgcccg
     1321 cgttccgcgc gtcgccgagg tgaagccgcg cgtgcgtccg caggaaccgc ggcaggaagc
     1381 gccgcgcacc gtcaccctgt ccgatccgcg cgtcacccag ccgagcctga gccgctacta
     1441 cctcgtgccg tcatatagct cgggccgtcc cggcgaggcc gagtcgatcg atgtgctcac
     1501 gcacgtcctg ggacggggcg ccaacagccg tctctacaag aagctcgtgg tcgataccgg
     1561 aatcgccgtc agcgccggcg ccagctacta cggcaccgcg ctcgactcga cccgcctcgg
     1621 cgtctacgcg acgccgaagc cagggaccag tctgacccag gtcgaggaag cgatcgatgc
     1681 ggtcctcacc gagctgattg aaaagggcgt gacccaggaa gaactggagc gttcccgcaa
     1741 ccggctgatc gccgacgccg tctatgcgca agacagccag agctcgctcg cgcgctggta
     1801 cggtgcatcg ctgaccactg gactgaccat cgaacagatc aggagttggc ccgatcgcct
     1861 gcgcgcggtc acggtcgacg atgtgaagaa cgccgcgaag caagcactcg acaaacggcg
     1921 ttcggtgacg ggatatcttg tcaggcaggc cggatcgagg gacgaccgct cgtgatcaat
     1981 cagcaagcaa tgctcctgtt cgcccgctct gcggttgcca ggctcggcgc gatgtcgctc
     2041 atcgtcgctg cgctggtcgc gctctgcgtg ccggccagtg ccaccaagat cgagcgcgtc
     2101 gtcagcccaa gcggcatcga attctggttc gtgcgcgacg ccaccgtgcc gctggtctcg
     2161 atcgaattcg cattccgcgg cggcgccgtg caggaccccg acgacaaaat gggtctcgcc
     2221 gagatgacgg tttcgaccct cgacgagggc gcgggcgatc tcgatggaac ggcattccat
     2281 gcgcggatgg aacgcaatgc catcgagctg cagattcgtg cgagccggga acacgtgcgc
     2341 ggcacgctgc ggacgctcaa ggagaaccag gacgaggcgt tcaacctgct gcggctggcg
     2401 ctgaacgagc cgagattcga gtcatccgcc gtcgagcgca tccgctcgca
//
LOCUS       DASRJQ010000451         4924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_429493, whole genome shotgun sequence.
ACCESSION   DASRJQ010000451 DASRJQ010000000
VERSION     DASRJQ010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4924
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429493"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VFB89_10195"
     CDS             <1..307
                     /locus_tag="VFB89_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27718.1"
                     /translation="AHRTGCELRATGKLSVRPDTAGSRSVDGDVAAGLEAAGWADIPR
                     YTADGPDGGSFGRRSRETVCLFRFSWDGGDDSDSTYVPSDDWEVVGHCGTREAGDSV"
     gene            complement(314..1606)
                     /locus_tag="VFB89_10200"
     CDS             complement(314..1606)
                     /locus_tag="VFB89_10200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZM27719.1"
                     /translation="MAVPIEQAVVPPSERVNILLVDDQPPNLVALEAMLEQLDQNLVP
                     ARSGLEALKWLLTNDAAVILLDVAMPDMDGFETAALIRERERSRHTPIIFLTAAAQYQ
                     TEAVRGYALGAVDYLLKPVVPEFVRSKVSVFVELAKQRSLLAQQADLLREKEQAARRL
                     AEMRAALVRDLEFKNRELESFSYAISHDLRAPIRRIESFAKALGETQASVLDQRGGKY
                     LGRIQESATQMLQLIDDVLHLSRVTIAELRCRDLDLSAMAQAIADRLRQDEPDRQVEV
                     RVRPGLNVTADPALLRVALENLLSNAWKFTSRTAAPVIEIGLMPSPDQSTYFVRDNGA
                     GFEMAQADKLFRPFQRLHLASEYPGTGIGLATVRRIIHRHGGSIWAQSMPGHGACFYF
                     TLNQPAVTDGAEAPSEEQELGAANGRAWSAAMNEVTTT"
     gene            complement(1591..>4924)
                     /locus_tag="VFB89_10205"
     CDS             complement(1591..>4924)
                     /locus_tag="VFB89_10205"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZM27720.1"
                     /translation="AGVWKDLTDNVNSMAANLTTQVRGIAKVVTAVANGDLKQKLVVE
                     TKGEIATLADTINGMIDTLATFADQVTDVAREVGIEGRLGGQARVPGAAGMWRDLTDN
                     VNQLAANLTAQVRAIADVATAVTKGDLTREIAVKAQGEVAELKDSINEMIRNLRDTTR
                     KNTEQDWLKTNLARFSRTLQGQRDLAAVSRLILSDLAPLVHAQQGLFYVVDSSGEEQS
                     LNLLGSYAHSDSGFTPRRFRMGEGLIGQCAGDRRSLHLTDLPPEYLTLQSGLGEAPPR
                     DLVVLPVLFEGEVKAVLELASLERFDEIRLNFLEQLAESIGIVLNTIEANMRTEGLLQ
                     QSQALTEGLQEQQEALTETNKRLEQQTRSLQQSEELLKKQQDELQQTNLELEDKAQLL
                     SSQKAELEQKNSEIEQARVALEDKAEQLALISKYKSEFLARMSHELRTPLNSLLILSQ
                     ILTENSEGNLTSKQVGFAQTIHSSGSDLLDLINDILDLSKIESGTMAIDISPVPFVEI
                     RKFVESTFRQVADVRGLAFDVELDESLPPVMQTDSKRLQQVLKNLLSNAFKFTERGKV
                     TLRASVATSGWNPEQDILNAASAVVAFTVSDTGVGIPKEKHGAIFEAFQQGDGTTSRK
                     YGGTGLGLSISRQITRLLGGEIRLQSAPGEGSSFTLYLPVTYPSHPAETPAAPEIPDA
                     VGQTGEYSFKPGTPSSRTMLAPVVERAVNDDRGSIQPGDRVLLIVEDDPHFAEILLDL
                     ARERGFKGLVTHRADKALLLAREYLPAAITLDLRLPDADGWTVLDRLKHDPATRHVPV
                     HVMSVDEDWQRGLRFGAIDFLTKPVTKESLSGALDHLHAFIDRPARRLLVVEDDEAQR
                     RSIGELIGGPDVEIEGAATGARALEMLHDGSFDCMVVDLGLPDMTGFDLVQRVKSEVG
                     LRKLPTIVYTGKDMTKKEELELRRLAESVVVKDARSPERLLEETALFLHRSAETMPDQ
                     QRQTLERLHKANPVLAGRRVLIVDDDVRNIFALTTFLEQSRMQVFYAESGREGIARLQ
                     ETPGIEAVLMDVMMPELDGYETIRSIREIPQFRGLPIIAVTAKAMKGDREKCIEAGAT
                     DYIPKPVDRDHLLSLLRVWLSR"
BASE COUNT          831 a         1604 c         1546 g          943 t
ORIGIN      
        1 ggcccatcgt accggctgcg agttgcgggc aacggggaag ctttccgttc gacctgacac
       61 ggcaggcagc cgcagcgtgg atggcgatgt ggcagcaggg ctggaagcgg cgggatgggc
      121 cgacatcccg aggtacaccg cagacggccc cgacggaggc tccttcggaa ggcgcagccg
      181 cgaaacggtg tgcctgttcc gtttcagctg ggatgggggc gacgacagcg actcgaccta
      241 tgtcccttcc gacgactggg aggtcgtcgg tcactgcggg acgcgggaag caggcgattc
      301 ggtgtagccg gcgttaggtg gtggtcacct cgttcatcgc agccgaccac gctcgtccgt
      361 tcgccgcgcc taactcttgt tcttcggacg gcgcttcggc accatctgtg accgccggct
      421 gattgagggt gaaatagaag caggcaccat gcccaggcat cgactgggcc cagatgcttc
      481 ctccgtgccg atggatgatc cgccgcactg tcgccaagcc gatgccggtg ccggggtact
      541 cgctggcgag gtggaggcgc tggaagggac ggaacagctt gtcggcctgg gccatctcga
      601 aaccggcccc gttgtcacgc acaaaatagg tcgactgatc gggactgggc atgaggccga
      661 tctcgatcac gggcgccgcg gttcgcgacg taaacttcca cgcattcgag agcagatttt
      721 cgagcgcaac cctgaggagc gcggggtccg ctgtcacatt gagccccggc cgcacccgca
      781 cttccacctg ccgatccggc tcgtcctgcc tcagccggtc agcaatggcc tgcgccatcg
      841 cactcagatc caggtcccgg cagcgcagct ccgcaatggt gacgcgggag agatgcagca
      901 cgtcgtcgat cagctgaagc atttgagttg cggactcctg aattctcccc agatactttc
      961 cgcctcgctg atccagtacc gaggcctggg tctcccctaa tgccttggca aaactctcga
     1021 tccgccggat cggtgccctc aggtcgtgag agatcgcgta gctgaagctc tcgagctccc
     1081 ggttcttgaa ttcaagatca cgcacgagag ccgcccgcat ctcggcgagg cgccgggccg
     1141 cctgctcttt ttcgcggaga aggtcggcct gctgggcaag tagcgatcgc tgcttggcca
     1201 gctcgacgaa gaccgacacc ttggagcgaa cgaactcggg cactacgggc ttgaggagat
     1261 agtccaccgc gccgagcgcg tagccccgca ccgcctcggt ctgatactgc gcggcggctg
     1321 taagaaagat gatcggcgtg tgccgagacc gttcgcgctc gcgaatgaga gccgcggtct
     1381 cgaagccgtc catgtcaggc atggcgacgt cgagcagaat aaccgccgca tcgttggtca
     1441 acagccactt cagagcctcg agccccgagc gcgccggaac caggttttga tccagctgct
     1501 cgagcatggc ttcgagcgcc accaggttgg gcggttgatc gtccaccagg aggatgttga
     1561 cccgctcgct tggcgggacc acggcctgct ctatcgggac agccatacgc gcagcagcga
     1621 cagcaggtga tcgcggtcca ccggcttcgg gatgtagtcg gtggcgccgg cctcgatgca
     1681 cttctcacgg tcacctttca tcgcctttgc ggtcaccgcg ataatgggaa gcccgcggaa
     1741 ttgaggaatt tcgcggatcg accgaatggt ttcgtacccg tccagctcgg gcatcatcac
     1801 gtccatcagg accgcttcga tccccggcgt ctcctgcagc cgcgcgatgc cctcgcgccc
     1861 gctctccgcg tagaacacct gcatccggct ctgctcgagg aatgtcgtga gcgcgaagat
     1921 gttgcgcaca tcgtcgtcca cgatgagcac acgccgtcct gcaagcactg gattggcctt
     1981 gtggagacgc tcgagcgtct gcctctgctg atcgggcatc gtctccgccg accgatggag
     2041 gaagagtgcg gtctcctcca gcagccgctc cggcgaccgg gcgtccttga ccactactga
     2101 ctctgccagg cgccgcagct ccaactcttc cttcttcgtc atgtctttgc cggtgtagac
     2161 aatcgtcggc agcttgcgaa gaccaacctc gctcttgacc cgctgcacca gatcgaaccc
     2221 cgtcatgtcc ggcagcccga gatcgaccac catgcagtcg aagctgccat cgtgcagcat
     2281 ctcgagtgcc cgcgcgccgg tagccgcacc ctcgatctcc acgtcggggc ctccgatcaa
     2341 ctctccgatg ctccgccgct gggcctcgtc gtcttcgacc accaggagcc gccgggccgg
     2401 gcgatcgatg aaggcgtgca ggtgatcgag cgcgccggac agcgattcct tcgtaaccgg
     2461 cttggtcagg aagtcgatgg cgccgaatct aaggccgcgc tgccagtcct cgtccaccga
     2521 catgacgtga accgggacgt gacgcgtcgc cggatcgtgc ttcagtcgat cgagcacagt
     2581 ccagccatcc gcgtcaggga gcctgagatc gagcgtgatc gcggcgggca ggtactcgcg
     2641 ggctagcagg agggccttgt ccgcccgatg ggtcaccagg cccttgaagc cgcgctctcg
     2701 cgccaaatcg aggagaatct ccgcaaagtg cgggtcgtcc tcgacgatga gcaggaccct
     2761 gtctccgggt tggatcgacc cgcggtcgtc gttcaccgca cgctcgacca ccggcgccag
     2821 catcgtccgg gacgagggcg tgcccggctt gaagctgtac tcgcccgtct gaccgacagc
     2881 gtccggaatc tcgggcgcgg ccggcgtttc cgccggatgg ctcggatagg tgacgggcag
     2941 atacagagtg aagctgctcc cctcgcccgg ggcgctctga agccgaatct ctccgccgag
     3001 aagccgggtg atctggcggc tgatggaaag accgagaccg gtgccgccgt acttccggct
     3061 ggtggttccg tccccctgct ggaatgcctc gaaaatcgcc ccgtgctttt cctttggaat
     3121 tcccacaccg gtgtcgctca cggtaaaggc cacaacggcg gacgcggcat tcaggatgtc
     3181 ctgctccggg ttccagcccg acgtcgccac gctggcgcgc agcgtaacct tgccccgctc
     3241 agtgaacttg aaagcattgg agagcaggtt cttgagcacc tgctggaggc gcttgctgtc
     3301 ggtctgcatg accggcggga gtgactcgtc aagctcgaca tcgaacgcaa ggccacgaac
     3361 gtcggccacc tgccggaagg tgctctctac aaacttccgg atctcgacga agggcaccgg
     3421 gctgatgtcg atcgccatgg tgccggactc gatcttcgag agatccagga tgtcgttgat
     3481 gaggtccagc aggtccgacc cggaagagtg aatggtctgg gcaaatccga cctgcttcga
     3541 cgtgagattg ccttcggagt tctcggtgag gatctgggag agaatcagca ggctattgag
     3601 cggcgtccgg agctcgtgcg acatgcgagc caggaactcc gacttgtact tggaaatgag
     3661 cgccagctgc tccgccttgt cctccagcgc cacgcgggcc tgctcgattt ccgagttctt
     3721 ctgctccagc tccgccttct ggctggacag gagctgcgcc ttgtcttcca gctccaggtt
     3781 ggtctgctgc agctcgtcct gctgtttctt gagtagctcc tcggactgct gaagcgaccg
     3841 ggtctgctgc tccagtcgct tgttggtttc cgtgagggct tcctgctgct cctgcagacc
     3901 ctcggtgagc gcctgcgact gctggagcag cccctccgtg cgcatattcg cttcaattgt
     3961 gttgagcacg atgccgatgc tctcggccag ctgctcgagg aagttgaggc ggatctcatc
     4021 gaaacgctcg agacttgcga gctcgagcac cgccttgacc tcgccttcga acagcaccgg
     4081 caggacaacc agatctcgtg gcggagcctc gccgagcccc gactgcaacg tgaggtactc
     4141 gggcggcagg tcggtgaggt gcagcgagcg acggtcgccg gcgcactgac cgatgagccc
     4201 ctcgcccatc cggaaccggc gcggtgtgaa accggaatca ctgtgcgcgt agcttcccag
     4261 caggttgagc gattgttcct ctcccgagct gtccactacg tagaagagac cctgctgtgc
     4321 gtgcaccaga ggtgcgaggt ctgacaggat gagccgggaa acggcggcca ggtctcgctg
     4381 accctgcagg gttcgactga agcgggcgag gttggtcttg agccagtcct gctcggtgtt
     4441 cttccgcgtc gtatcgcgga ggttgcggat catctcgttg atgctgtcct tgagctcagc
     4501 cacctcaccc tgcgccttca ccgcgatctc tcgagtcagg tcgcccttgg tgacggccgt
     4561 cgccacgtcc gcaatggctc gaacctgggc cgtaaggttt gccgccaact gattcacgtt
     4621 gtcggtgagg tcgcgccaca tcccggccgc acctggcacg cgcgcctgcc cgcccagccg
     4681 gccctcgatg ccgacctcgc gtgccacgtc ggtcacctga tccgcgaacg tcgcgagggt
     4741 atcgatcatg ccgttaatgg tgtccgccag cgtcgcgatc tctcccttgg tttccaccac
     4801 cagcttctgt ttgaggtcac cattggcgac ggccgtcact accttcgcga tgccgcggac
     4861 ctgcgtcgtg agattggccg ccatcgagtt cacgttgtcg gtgagatcct tccagacgcc
     4921 cgcc
//
LOCUS       DASRJQ010000452         3613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3404542, whole genome shotgun sequence.
ACCESSION   DASRJQ010000452 DASRJQ010000000
VERSION     DASRJQ010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3404542"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1630)
                     /locus_tag="VFB89_10210"
     CDS             complement(<1..1630)
                     /locus_tag="VFB89_10210"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZM27721.1"
                     /translation="MSHVWDRMESAPDLARLTLICFGPPTARLSGRDAPAEVLWRKNL
                     ALLIYLALAPQYRRSRNQILRALWPDKDESRARHSLNESVHRLRSSLGQARLESQGDC
                     LVLSCEGLDVDALRFTALATTKPADAVGLLHGDFLEGFTVEDAPEFEDWASVERAGFR
                     AKAGAALVALGESLLLDGRSRLAGARDAARQARTFDQYSEPAVRLEMRAAALAGDQAG
                     ALKAHHEFILALREINEQPSRDLAALADRIRSDRWRQAAQATRLDDSALVGRAAETAS
                     LARMLAEGMASGPRIIAITGDPGTGKTRLLSHSLERLALDGALIIAARPLEGDRDTSW
                     STLRALTRAGLLNAPGLAATDPHGLAVLASLIPELASRIEPREPMDRGQIAAALSSVL
                     EAIADEIPVALALDEAQYADGATVGALHAAVAQLRVTPVVVILTSTGSPESATNELLQ
                     LHRDIGRGLAGTVVQLEPLTLADIGELVVALAPWCCSTSEQQRLARRVAFETRGNPFL
                     AVTLLRGLQDFVLLRSEALAWPPPQATLESPLPMA"
     gene            complement(1704..>3613)
                     /locus_tag="VFB89_10215"
     CDS             complement(1704..>3613)
                     /locus_tag="VFB89_10215"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM27722.1"
                     /translation="RFPTARAFARALQPPVARSVPTVVTPPAATPAQAAEIPSMSPSP
                     SPAAVRRRFSYVPTAVVMLGLGFMIGGGALFAWRFTHPSAEYDGPKRLAVLPFENLGD
                     SVDAYFADGITDELRSSLATVPGLEVVASGSSNQYRRTTKPLSEIARELGADYLLVGT
                     IRWQRADGGPSRVRVSPELIKVGPGSVPTTTWAQPFEAARTDVFQVQVDIAARVTQSL
                     PGSWTELARGPPRRPTMNTDAYDYYLRGNEYYARETMADVQLSIQLYRRALTFDSAFA
                     LAWARLARAEAFVYWFAGDRSTAQLARVEESARKALSLAPDLPEAHIAMGYYHYWGHR
                     DYSAALGEFASAAKSEPNNAEVADVVGLVLRRRGEWDEAVANFRRAAALDPRSVEYLF
                     DLAHTYFLMRRYPDAERALGRAAALAPDSPNIYALRMATSVNAEGNLDKPRRLMREAL
                     TRVDFDRFGSTRLFGDCFELIASDDEYQGELARLTPAAFNGVALLYFEFKASVYRRRA
                     DSSKARAYGDSARIEALATIQRHEGDVFSYSIVALENAYRGRGAEAVEAGKQALKVLP
                     QSKDAVLAPQAHIALAEVYMVLGDTTGALVQLRAALSVPSYLSAGRLRADPVWAGLIA
                     TPGFKEMMEQS"
BASE COUNT          689 a         1154 c         1156 g          614 t
ORIGIN      
        1 ccgccatcgg gagcgggctt tccagtgtgg cctgcggcgg cggccacgcg agtgcctcgg
       61 aacggagcag cacgaagtcc tgtagcccac gaaggagcgt gactgcaaga aacggattgc
      121 cgcgggtctc gaacgcgacc cgtcgagcga gtcgttgttg ttccgacgtg ctacagcacc
      181 atggggctag cgcgaccaca agctcaccga tatcggccaa agtgagcggc tccagttgca
      241 ccacggtacc ggccaggccg cggccgatgt cgcgatgcag ctggagcagc tcgttcgtcg
      301 ccgattccgg gctgccggtc gatgtcagga tgacgacgac cggcgtcacc cggagttgtg
      361 ccacagccgc gtggagtgca cccaccgtgg cgccatcagc gtactgcgct tcatcgagcg
      421 ccagggcaac tgggatttca tcggcgatcg cttcgaggac ggagcttaat gccgccgcaa
      481 tctggccccg atccattggc tcacgtggtt cgatccggct ggccagttcc gggatcagcg
      541 aggccaatac tgccaatcca tgcggatctg tcgccgctaa gcctggtgca ttcagcagac
      601 ccgcacgcgt aagcgcgcgc agagtgctcc aggaggtatc ccggtcgcct tcgagagggc
      661 gagccgcgat gatgagagca ccgtccaacg caagtcgctc gagagagtgg ctcaggagcc
      721 gcgtctttcc ggtgcccgga tcgccggtta ttgcgattat gcgcggacct gatgccatcc
      781 cttctgccag catccgggcc aggctagctg tctcggcagc acggccgacc aatgctgagt
      841 catccaggcg ggtggcttgt gctgcttggc gccatcgatc gctccggatt cggtccgcca
      901 aggcagcgag gtcacgactc ggttgctcat tgatttcgcg cagggcaaga atgaactcat
      961 ggtgcgcctt gagagcacca gcctgatctc cggccagcgc agccgcgcgc atctccaagc
     1021 gtacagcggg ttcggaatac tggtcgaagg tgcgcgcctg ccgcgctgca tctcttgccc
     1081 cggccaatcg gcttctgccg tcgagcagaa gactctcgcc aagcgcgacc aaggccgctc
     1141 ctgccttggc gcggaatccg gcgcgttcca cggacgccca gtcctcgaac tctggtgcgt
     1201 cctcgacggt aaatccctcg aggaaatcgc catgcaggag cccgacggca tcggcaggct
     1261 tggtcgtagc gagagcagtg aaccggaggg catcgacgtc caaaccctca cagctcaaga
     1321 caaggcagtc accctgggac tcgagccgag cctgacccaa gctgctgcgc aagcggtgca
     1381 cagattcgtt caacgagtga cgggctcggg attcatcctt gtcgggccag agggcgcgaa
     1441 gaatctgatt tctggatcgg cggtactggg gagcgagggc gagatagatg aggagggcca
     1501 ggttcttccg ccagagaacc tcggccggcg catcccggcc actgagccgg gcagtgggcg
     1561 gcccgaagca gatgagcgtg aggcgagcca gatccggagc ggactccata cgatcccaaa
     1621 catgtgacat tgctccggcc cgtccagatg cgactatgtc gctgcgtctg ccatccccat
     1681 gattgccatt tacgcttcgc atcttagctc tgctccatca tttccttgaa tcccggcgtc
     1741 gctatgagcc ccgcccaaac gggatcagca cgaagccgcc cagctgagag ataagacggt
     1801 acggagagtg cggctcgcag ctgaaccaga gcgccagtcg tatcccctaa caccatgtag
     1861 acctccgcca gggcgatgtg agcctgcgga gccaacacgg catccttcga ttgcggcagc
     1921 accttcagag cctgctttcc tgcctcgaca gcctctgcac ctcgtccccg gtacgcgttt
     1981 tctaacgcca ctatggagta gctgaagaca tcgccttcgt ggcgctgaat ggtcgccagg
     2041 gcctcaatgc gcgccgagtc cccgtaggct cgtgccttgc tggaatccgc gcgacgccga
     2101 tagaccgatg ccttgaactc gaagtagagt agcgcaacac cgttgaaggc ggctggggtg
     2161 aggcgcgcaa gctcgccttg atactcatcg tcagaggcga tgagctcaaa gcagtcaccg
     2221 aagaggcggg tcgaaccaaa ccggtcaaag tccacacggg tcagagcctc gcgcatcagc
     2281 cggcgtggct tgtcgagatt accctccgcg ttaacggagg tcgccatacg gagggcatag
     2341 atgttgggtg agtccggcgc cagggcagca gcgcggccga gcgcccgctc ggcgtccggg
     2401 tagcgccgca tgagaaagta ggtgtgggca agatcgaata gatactcgac cgaacgagga
     2461 tccagcgccg ccgcacgccg gaaattcgcg acagcttcat cccactcgcc ccggcgccgc
     2521 agcacgagac cgaccacgtc ggcaacctcc gcgttgttcg gttcgctctt cgcagcgctg
     2581 gcgaattcgc ccagcgcggc cgaatagtcc cggtgtcccc aatagtggta gtagcccatc
     2641 gcgatatggg cttcgggaag gtcaggtgcg agactcaagg ctttgcgagc agattcctcg
     2701 acccgtgcca gttgagccgt cgagcgatca cccgcaaacc agtacacgaa ggcctcggcg
     2761 cgggcgagtc gtgcccaagc cagtgcaaac gcggagtcga aagtgagagc acgtcggtac
     2821 aactgaatgc tcagctgcac gtcagccatc gtctcgcggg cgtaatactc gtttcctcgg
     2881 aggtagtagt cgtacgcatc ggtgttcatg gttggccgcc tgggagggcc ccttgcaagc
     2941 tctgtccatg atcccgggag cgactgagtg actcgggccg cgatatccac ctgaacctga
     3001 aacacgtcgg ttcgcgccgc ctcgaacggc tgggcccaag tcgtggtcgg cacgctgccg
     3061 ggcccgacct tgatcagttc gggactcacc cgtacccggc ttgggcctcc gtccgcccgc
     3121 tgccagcgga tcgtcccgac cagcaggtag tccgctccca gctctcgggc gatctccgac
     3181 agcggcttgg tggtccggcg gtactgattg gagctcccgc tcgcgacgac ctcgagtccc
     3241 gggacggtcg ccaggcttga gcgaagctca tcggtgatcc cgtcggcaaa gtaagcatcg
     3301 accgagtccc ccaagttctc gaatgggagc acggcaaggc gcttgggccc gtcatattcc
     3361 gcgctgggat gggtgaagcg ccaggcgaac aaagcgcccc ctccaatcat gaatcccagg
     3421 ccgagcatga caaccgcagt aggcacatag gaaaaccggc gccgcaccgc cgcgggcgac
     3481 ggcgatggcg acatggaagg aatctccgcc gcctgggccg gagtcgcggc cggaggcgtg
     3541 acgaccgttg gtaccgacct ggcgacgggg ggctgaagtg cgcgagcgaa ggcgcgggca
     3601 gtcgggaatc gat
//
LOCUS       DASRJQ010000453         4522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_4095955, whole genome shotgun sequence.
ACCESSION   DASRJQ010000453 DASRJQ010000000
VERSION     DASRJQ010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4095955"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VFB89_10220"
     CDS             <1..438
                     /locus_tag="VFB89_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27723.1"
                     /translation="ADQHRGTPVWLATWLATAVVALAIGGGAMAAKAKRAGTSVFCYS
                     GRRFVLSYIPPFLVGILLTIVLARAGLAAALPGTWLLLYGTGVVTGGAYSVRAVPLMG
                     LCFMALGAIALFTPVRWGDALMALGFGGLHIGFGLVIARRHGG"
     gene            431..793
                     /locus_tag="VFB89_10225"
     CDS             431..793
                     /locus_tag="VFB89_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HZM27724.1"
                     /translation="MAEPRAARPDPEDSGIAHVLRQDALELDRLIHERMRLAIVSALA
                     VNESLTFNELKRLLETTDGNLSVHARKLEEARYITCTKTFEGRLPRTEYRLSPAGRQA
                     LERYLEHMEALIRTMRDR"
     gene            819..1553
                     /locus_tag="VFB89_10230"
     CDS             819..1553
                     /locus_tag="VFB89_10230"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF011412.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HZM27725.1"
                     /translation="MKPSTPSPSAPSGLHVAIIMDGNGRWATRQGKPRSAGHREGAEV
                     VQRIVEVAPDLGVGTLTLFAFSADNWQRPAREVKLLMRLFAEYLRAEAARCVSRGVRL
                     TVLGRRDRLSPSLLSAIDYTESATSGGTRLLLRLAIDYSARDAILRAAQCLSPDNVLS
                     RESFSRLLTIVDHGGPHASPVDLLIRTGGERRLSDFLLWESAYAELCFTPVMWPDFGP
                     DHLRQAVRDFHGRERRFGGLPDAAAS"
     gene            1780..2016
                     /locus_tag="VFB89_10235"
     CDS             1780..2016
                     /locus_tag="VFB89_10235"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZM27726.1"
                     /translation="MIDPLTCQDAVARLEDYLDRELNEEEMRQVRHHLEICAACAGHF
                     RAEEAVLQQLRDKLRRIAVPADLMARVQKKLGGG"
     gene            2099..2563
                     /locus_tag="VFB89_10240"
     CDS             2099..2563
                     /locus_tag="VFB89_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27727.1"
                     /translation="MSDAEARRLERDAERLENWKRWGPYLSERQWGTVREDYSAYGNC
                     WEYFPHDHARSRVYRWGEDGLLGFTDRECRLCFSVALWNGHDPILKERLFGLTGPEGN
                     HGEDVKECYYYLDSTPRTPTPKHSTSTRSLASPMTSSWPRIGDARKPIPSSS"
     gene            2515..>4522
                     /locus_tag="VFB89_10245"
     CDS             2515..>4522
                     /locus_tag="VFB89_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27728.1"
                     /translation="MAENRRRSKADPEFELTDTGVFDESRYFDVFAEYAKASTDDILI
                     RLTIANRGPDPARIAVLPTLWFRNVWSWGRVGEGYGPRSRLEKLGGNAVVAQNPTLGD
                     YRFDAEPAANGPEWLFTENETNVARLYGVSNSHPFVKDAFHDYMVGGRTEAVNPARVG
                     TKAAALYWFTIDAGAQVTIRLRLRQVEAPADAFGPAFDRVFETRIREADEFYRNRLPE
                     QVTAEERAVLRQGYAGLLWSKQFYHYVMNDWLEGDPAQPPPPESRKSGRNNEWSHVFN
                     RDVISMPDKWEYPWFAAWDLAFHMIPFARIDPQFAKEQLLLLLREWYTHPNGQIPAYE
                     FAFSDVNPPVHAWAVWRVYKMTGARGKRDRLFLERAFHKLLLNFTWWVNRKDPEGRNL
                     FAGGFLGLDNIGVFDRSRPLPTGGHLEQADGTAWMAFYCNTMLSIALELACEDPAYED
                     VASKFFEHFIAISDAINSLGGTGLWVDDDGFYYDQLHVDSSVVPLRVRSLVGIIPLIA
                     VEVLDDVRINELRGFRKRMEWFLVNRPDLARHISCMHPRMATSGAHRLLAIPSRDRLT
                     RLLRYVLDETEFLGAHGIRSVSRVHRDHPYVFDVMGERYEVEYVPGESNTGLFGGNSN
                     WRGPIWFPINFLVLEALERYGHFYGDDLKVECPLGSGQMMSLQEV"
BASE COUNT          767 a         1438 c         1452 g          865 t
ORIGIN      
        1 gccgaccagc atcgggggac gccggtctgg ctcgcgacct ggctcgccac ggcggtggtc
       61 gccctcgcca tcggcggcgg ggccatggca gcgaaggcaa agcgggcagg gacctcggtc
      121 ttctgttatt ccgggcggcg cttcgtcttg agctacatcc cgccttttct ggtcggcatt
      181 ctgctcacca ttgtgcttgc acgtgcagga ctcgcggcgg cgcttccggg tacctggctg
      241 ctcctctacg gcaccggtgt ggtgaccggc ggcgcctact cagttcgcgc cgtgccgctc
      301 atggggctct gcttcatggc cttgggcgcg attgctctct tcactccggt ccgctggggc
      361 gatgccttga tggcgctcgg cttcggcggg cttcacatcg gattcggcct cgtgatcgcc
      421 cggaggcacg gtggctgagc caagagctgc ccggcccgat ccggaagact cggggatcgc
      481 gcacgttctc cggcaggatg cgctcgagct ggaccggctc atccacgagc ggatgcggct
      541 ggccattgtg agcgcgttgg cggtaaacga gtccctgacg ttcaacgagc tgaagcggct
      601 gctcgagact accgacggca acctgagcgt gcacgcgcgg aaactggaag aggcccggta
      661 catcacttgc accaagacgt tcgaaggtcg gctcccccgg actgaatacc ggctgagccc
      721 cgcgggacgg caggcgttgg agcggtacct cgaacacatg gaagcactga ttcgcaccat
      781 gcgggaccgc tgatcccgtc tcccggagct caactctgat gaagccaagc acaccgtccc
      841 cgtcggctcc ttccggattg cacgtcgcca tcatcatgga cggaaacggc cgatgggcca
      901 ctcggcaggg caagccgcgt tcggccgggc atcgcgaggg tgccgaggtg gtccagcgga
      961 tcgtcgaggt cgccccggac ttgggcgtcg ggaccttgac gctctttgct ttctccgccg
     1021 acaactggca gcggccggcc cgggaagtga agctcctgat gcggctcttc gcggagtacc
     1081 tcagggccga ggccgctcgc tgcgtctcgc gtggagtgcg gctcaccgtg ctgggccggc
     1141 gcgaccggct ctcgccgtcg ctgctcagcg cgatcgacta caccgagagc gcgaccagcg
     1201 gcggcaccag gctgctgctt cgcctcgcga tcgactactc tgcccgggac gcgattctgc
     1261 gggcggctca gtgcctgagc cccgacaacg tgctctcgcg ggagtcgttc agccgactcc
     1321 tcacgatcgt cgatcacggg ggaccgcacg catcgccggt ggatctcctc atccgcaccg
     1381 gcggcgagcg ccgcctgagc gacttcctcc tctgggaatc ggcctacgcc gagctgtgct
     1441 ttactcccgt catgtggccc gacttcggcc ccgatcacct gcgtcaggcc gtccgtgact
     1501 tccacgggcg ggagcgcagg ttcggcgggc tgcccgacgc cgcggcaagc tagcagctag
     1561 cagctatcag ctatcagcta tcagctatca gaggcttcct gggatggccg tggctccgct
     1621 atcctgcctt actatagctg atagctgata gctgatagct cttctttgga actaatcgcc
     1681 aactagtgac gttatccccc ctggtttccc cgaattgagt tcaatcgcgg gggcaggcag
     1741 gctagtggat cgctacgctc ggaggcatcg tgaagtcaga tgattgaccc cctgacctgc
     1801 caggatgccg ttgcccgcct cgaggactac ctcgaccgag agctaaacga ggaagagatg
     1861 aggcaggtcc ggcatcatct cgagatctgc gcggcctgtg ccggccattt cagggccgag
     1921 gaggcggtgc tgcagcagct gcgggacaag ctgcggcgaa tcgcggtccc tgccgacctc
     1981 atggctcggg ttcagaagaa actgggaggc ggataggcgg ccgttgaccc tgccggccag
     2041 gggaaccatt tgctgcatcg gtacattcca tcctcagtct ctactggaag cagcatctat
     2101 gtccgacgcc gaagcccgcc gtctcgaacg tgacgctgaa cgactggaaa actggaagcg
     2161 ctggggcccc tatctgtccg agcgacagtg gggtacggtg cgtgaggact attctgccta
     2221 cgggaactgc tgggagtact ttccgcacga ccacgctcgg agccgggtct atcgctgggg
     2281 cgaggacggc ctgctcggct tcacggatcg cgagtgccgg ctctgtttca gtgtcgcgct
     2341 gtggaatggc catgacccga ttctcaaaga gcggcttttt gggttgaccg gaccggaagg
     2401 caaccacggt gaggacgtga aggagtgcta ctactacctc gactccaccc cacgcactcc
     2461 tacgccaaag cactctacaa gtacccgcag tctcgcttcc cctatgacga gctcgtggcc
     2521 gagaatcggc gacgctcgaa agccgatccc gagttcgagc tgaccgatac cggcgtgttc
     2581 gacgaaagcc gctacttcga cgtcttcgca gaatatgcca aggcatccac cgacgacatt
     2641 ctgattcggc tcaccatcgc gaaccgcggt cccgatccgg cccggatcgc agtcctgccc
     2701 accctctggt tcagaaacgt ctggagctgg ggtcgtgtgg gcgagggcta cggcccccgt
     2761 tctcgcctgg agaaactggg cggcaacgcg gtggtggcgc agaacccgac gctgggagac
     2821 taccgattcg atgccgagcc ggccgcgaac ggacccgagt ggctgttcac ggagaacgag
     2881 accaacgtcg cccggctcta tggcgtctcg aactcacatc cgttcgtgaa ggatgccttt
     2941 cacgactaca tggtcggcgg ccgaacggag gcagtaaacc cggcgcgggt tgggaccaag
     3001 gccgccgcct tgtactggtt caccatcgac gccggcgcgc aggtgacgat tcgtctgcga
     3061 ctgcggcagg tggaagcacc cgctgacgcg tttggaccag cgttcgatcg ggtcttcgag
     3121 acccgcatcc gcgaggcgga tgagttctac cggaatcggc tgccggagca ggtgacggcg
     3181 gaagagcgcg cggtgctacg ccagggctat gcgggcctgc tctggagcaa gcagttctat
     3241 cactacgtga tgaacgactg gctggagggg gatcctgccc aaccgccgcc gccggaaagc
     3301 cgcaagtctg gtcgcaataa cgagtggtcc cacgtgttca atcgcgacgt gatctcgatg
     3361 ccggacaagt gggagtatcc ctggttcgcg gcctgggatc tggccttcca catgatcccg
     3421 tttgcgcgga tcgatcccca gttcgccaag gagcagctcc tcctgctctt gcgcgagtgg
     3481 tacacccatc ccaacggtca gatcccggcc tacgagtttg ccttttccga cgtgaacccg
     3541 ccagtgcacg cctgggcggt gtggcgggtg tacaaaatga ccggcgctcg cggcaagcgc
     3601 gaccggctct ttctcgagcg cgcgttccac aagctgctgc tcaacttcac ctggtgggtc
     3661 aatcgcaagg acccggaagg tcgcaatctg ttcgcgggcg gcttcctcgg cctcgacaac
     3721 atcggcgtgt tcgaccggtc ccgtccgctg cccactggtg gtcatctgga gcaagccgac
     3781 ggcacggcct ggatggcgtt ctactgcaac accatgctct cgatcgcact ggagctggcc
     3841 tgcgaagatc ctgcctacga ggacgtcgcc tcgaaattct tcgagcattt catcgcgatt
     3901 tccgatgcga tcaattccct ggggggcacc ggcctgtggg tcgatgatga cgggttctat
     3961 tacgatcagc tccatgttga tagtagcgtc gtcccgctcc gggtgcgctc cctggtcggc
     4021 attattccat tgatcgcggt cgaagtactc gacgacgtga gaatcaacga gctgcggggc
     4081 ttccgcaagc gcatggagtg gttcctggtg aaccgccccg atctcgcgcg acacatctcg
     4141 tgcatgcatc cgcgcatggc aacgagcggg gcccatcggc tgcttgcgat cccgtcgcga
     4201 gaccgcctga cgcggctttt gcgttacgtg ctggacgaga ccgagttcct gggcgctcac
     4261 gggatccggt cggtttcgcg agtgcatagg gatcatcctt acgtcttcga tgtcatgggt
     4321 gagcgatatg aggtggagta cgttcccggc gagtccaaca ccgggctctt cgggggcaac
     4381 tccaactggc ggggccccat ctggttcccc atcaatttcc tggtgctcga ggcgctggaa
     4441 cgctacggac acttctacgg cgacgatctc aaagtggagt gtccgctggg gtccgggcag
     4501 atgatgtcgc tgcaggaggt gg
//
LOCUS       DASRJQ010000454         2149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3994423, whole genome shotgun sequence.
ACCESSION   DASRJQ010000454 DASRJQ010000000
VERSION     DASRJQ010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3994423"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VFB89_10250"
     CDS             complement(<1..288)
                     /locus_tag="VFB89_10250"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZM27729.1"
                     /translation="MTVSRLADTVQNGLEVAVLDRERELAEIDRALAAAIAGNGRVLL
                     VEGPAGIGKSTLLHAGSALAHDRGVTVLSGRGSPIERQFSYGVVRQLYEPFG"
     gene            412..918
                     /locus_tag="VFB89_10255"
     CDS             412..918
                     /locus_tag="VFB89_10255"
                     /inference="COORDINATES: protein motif:HMM:NF025196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4188 domain-containing protein"
                     /protein_id="HZM27730.1"
                     /translation="MNRVTRTTVDLTEYPDLVVIYLGMRAHSLRGFRTLGRVIGWIEE
                     AAAARPDGLLCHETFWFRLLPPHGGVRQYWRDFDALERWARSHPHGEWWATFTRDPQG
                     TSFWHETYFLQGGFEAIYDDVPEPLGLKSFAPVVPASGAMFSARRRAGLPGEPPAPVI
                     PEAEFERL"
     gene            943..1785
                     /locus_tag="VFB89_10260"
     CDS             943..1785
                     /locus_tag="VFB89_10260"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZM27731.1"
                     /translation="MTVQTDPTYALGRSKIEYERLTAQAALIRPMTERLFREAGIGPG
                     QRVLDVGSGVGDVAFLAAELVGPDGEVVGVDLDGAALATARGRADLLGFRNVSFIEGD
                     ARTAELGQGFDAAVGRLVLLYVADPSELLRAVADRLRPDGVLAFQELDMDPEVPSFST
                     APAQLWDETGHRIIQTFVQAGTRVRVGRELFTAFTNAGLPAPQVREETLIGGGPEFTG
                     YGWLAGVARSLAPLMERFGIATAAELDIDNLADRIRTDAVKHQSLVWAPPLIGAFARR
                     SSRR"
     gene            complement(1891..1964)
                     /locus_tag="VFB89_10265"
     tRNA            complement(1891..1964)
                     /locus_tag="VFB89_10265"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1930..1932),aa:Gly,seq:ccc)
BASE COUNT          360 a          708 c          710 g          371 t
ORIGIN      
        1 cccgaacggc tcgtacagct ggcggaccac cccgtaggag aactgacgct cgatgggtga
       61 gccgcgcccg gaaaggacgg taacgccacg atcgtgtgcg agcgcagagc ccgcatgcag
      121 cagcgtcgac ttcccgatac ccgcggggcc ttcgacgagc aggactcgcc cgttccccgc
      181 gatcgcagcc gcgagggcgc gatcgatctc cgcgagttct cgctcgcggt cgaggacggc
      241 tacctcgaga ccgttctgga ccgtgtcggc caggcgactt acggtcacgt ttccgattgt
      301 tgcaccgtcc gtaccacccg gccagcgacg atttcctcag tgcttcgagc accaaaactc
      361 agggggccga ccctgatgtt gccgacatcg gtccggcgcc atcctcgggg aatgaaccgc
      421 gtcacacgca cgacggtcga tctgaccgag taccccgatt tggtcgttat ctacctggga
      481 atgcgggcgc attccctgcg cggcttcagg acgctgggca gggtgattgg ttggatcgaa
      541 gaagccgccg ccgcgcgccc agacggtctg ctctgccatg agacattctg gttccggctc
      601 ctgccaccgc acggcggcgt ccgccagtac tggcgtgact tcgatgcgct cgaacggtgg
      661 gcccgctcac acccccacgg tgagtggtgg gccacgttca cccgcgaccc gcaggggacg
      721 agtttctggc acgagaccta cttcctgcag ggcgggttcg aggccatcta cgacgacgtg
      781 cccgaaccgc tcgggttgaa gtcgttcgca ccggtggtgc ccgcaagcgg ggcgatgttc
      841 tcggcgcgac gccgggccgg acttccgggc gagcccccag caccggtcat cccggaggca
      901 gagttcgagc ggctgtgacc gcgttgacga aagaaggaag ccatgaccgt tcaaactgac
      961 cccacgtacg cgctgggacg ttcgaagatc gagtacgagc gcctgaccgc gcaggccgcg
     1021 ctgatccgac ccatgaccga acgcctgttc cgagaggctg gcatcgggcc cggccaacgc
     1081 gtcctcgacg tgggcagcgg cgtaggagat gtggcgttcc tggcagccga actcgtcggg
     1141 cccgatggcg aggtcgtcgg cgtcgacctc gatggtgccg cactcgcgac cgcgcgaggc
     1201 agggccgacc tgctcgggtt ccggaacgtg agtttcatcg agggtgacgc ccgcacggct
     1261 gagcttggtc agggattcga cgccgccgtc ggtcggctcg ttctcctcta cgtcgccgat
     1321 ccgtcggagc tgttgcgggc ggtcgccgat cgcctgcgcc ccgacggcgt gctcgccttc
     1381 caggagctcg acatggatcc cgaggtgcca agtttctcca ccgcaccggc gcaactctgg
     1441 gacgagactg gccaccgcat catccagacg ttcgtgcagg caggcacccg tgtccgggtg
     1501 gggcgcgagc tcttcaccgc gttcacgaac gctgggctcc cagctcctca ggttcgcgag
     1561 gagaccctca tcggcggcgg accagagttc accggctacg gatggctcgc tggtgtggcc
     1621 cggagcctcg cgccgctcat ggagcgcttc gggatcgcga cggcggcaga gctggatatc
     1681 gacaacctcg ccgaccgcat tcggacggac gctgtgaagc atcaatccct ggtttgggca
     1741 ccgccgttga tcggtgcgtt cgcgcgcaga tcgagcaggc gatagccgaa gggcgaaagc
     1801 ccttgtccta ggcagtttct gcggacgtga agctgagggt ggagcttagg ggacccgaac
     1861 ccccaatcgc atccgaccag ggtaattctt tggagcggat gacgggaatc gaacccgcac
     1921 tgtcagcttg ggaagctgat gttctgccat tgaactacat ccgcctgacc aggtattttg
     1981 catctttagc cctggtgccg tgccgccgcg gtgcctcaag cttcggagcg acgaaacctc
     2041 aggctagcgc acgtcgggca ccgcgccgga gccgatttca ctttggccgg ccgccggctc
     2101 caggaacttc atcgcagaag ctggtgaacc tgatcgagcc ttgaaggcg
//
LOCUS       DASRJQ010000455         4587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1602200, whole genome shotgun sequence.
ACCESSION   DASRJQ010000455 DASRJQ010000000
VERSION     DASRJQ010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602200"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VFB89_10270"
     CDS             complement(<1..612)
                     /locus_tag="VFB89_10270"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZM27732.1"
                     /translation="MSAQLPTQLPRVVIVGGGFGGVAAAKALRRAPVEVILIDRNNHH
                     VFMPLLYQVATAVLAPGQVGYPIRGIVGKQMNTTVIMGEVTGVNKEQRYVVASSADRA
                     DVPIRYDYLILATGVSHSYFGHDEFAPYAPGLKTLADAEAVRNRILQAFEQAEAEEDP
                     SRHRDLLTFVLVGAGPTGVEMAGAIAVMVRTTLRSEFKRIDPRS"
     gene            complement(620..1669)
                     /gene="cydB"
                     /locus_tag="VFB89_10275"
     CDS             complement(620..1669)
                     /gene="cydB"
                     /locus_tag="VFB89_10275"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HZM27733.1"
                     /translation="MIPIWYAVVSFMLIAYVVLDGRNFGAGMLHWLVAKTPEERRQVI
                     AAIGPLWSWHEVWLVGFGGILVAVFPRLMASAFSGYYLALFLILWGLILRGISIEVGG
                     HINDRQWQGFWDFVFVCSNLLLATLFGAAAGNLARGVPLDAQGNFSMPFFTDFTVHGQ
                     VGLLDWYTISVAVFTVVLLAAHGATYLTLKTEGPVHDRSRQYMRYLWAAVVPLLLLIS
                     FESWVVRPDLPARAAANPVWWLGVLVVLGSAITVFSGLSARREMRAFIGSNFLLAGLL
                     AAGGAALYPVMLHSTLAPENSLTAQAVAASPRALALASMWWPVAFMLAIAYFVFISRR
                     YVGKVSVQRNSQGFY"
     gene            complement(1666..3069)
                     /locus_tag="VFB89_10280"
     CDS             complement(1666..3069)
                     /locus_tag="VFB89_10280"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HZM27734.1"
                     /translation="MNVTLSDPAFWSRFQFGFTLTYHYLFPQLTMGLAWFLVYWKWRA
                     LRTGDEKYNDAVRFWAKIFGLNFAVGVVTGIPMEFQFGTNWAGFSRYAGGVIGQTLAM
                     ESMFAFFLESAFIGALIWGEKRLGPRYHFLAAVAVALGSWISGFFILVTNAFMQHPVG
                     HRVEANGSLSIDSLGSYLLNPWALVQFTHNQVAALVTGSFVVAAMGAFYTLRWRHPVQ
                     ARLYLRAGTAVGLIASVLVAVPTGDQQAKMVGNHQPVTLAAMEGRFVGGPMAGVAVIG
                     QPNVAARRLDNPIEVPGALSFLAYGTFKSYVHGLQEYPETTWPDNIELLYYSFHLMIT
                     LGTLFILLMAYAGLQNWRGRLESSTGLLWVLMLAFPFPYIANTLGWMTAELGRQPWLV
                     YGLFRTNQGHSTVVSSGDTIFTLLGFIGLYFVLGLLFLYLVGREIGQGPDDDVRVNPG
                     DLQVGRAVPVRETVGTL"
     gene            complement(3086..3301)
                     /locus_tag="VFB89_10285"
     CDS             complement(3086..3301)
                     /locus_tag="VFB89_10285"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HZM27735.1"
                     /translation="MADSVYKVIELVGASSESWEKAAAAAVNQASKSLRDLRVAEIVQ
                     LDVTIENGKVKSYRAKVKLSFKYESGR"
     gene            complement(3322..>4587)
                     /locus_tag="VFB89_10290"
     CDS             complement(3322..>4587)
                     /locus_tag="VFB89_10290"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZM27736.1"
                     /translation="VIAVPLNTRLSSHEIDRVLADADPRGLIRHSSLPQPSTQVPWQV
                     VLDQEELDLGSGEPPEPVYDPNAILTLIYTSGTTGRPKGVMLTHAGVLATVEHLGAWM
                     EYREGGAWLHAAPIFHIADFPIIFAAPGFGACQVTIPKFTPQGFCDVVARERVTHTVL
                     VPTMINLLAQFPGITEYDLTSLEVLGYGGSPMAPELIHRTRTLFPGIKLIQVYGLSET
                     GFLTGLMDHEHTEQRLTSCGRPCIGIDVRVADETGKELGPGQTGELVARGANVMRGYW
                     NNPAETALAFRDGMFRTGDVGYQDGDGYFYILDRLKDMIVTGGENVYSGEVEAVIYEH
                     PAVREAAVFGIPDPKWGELVMACVVLKPGQTLTADELLAHCRQSLANYKVPRRVEFFD
                     TDLPKSSSGKILKRVLRERFWEHQERAVS"
BASE COUNT          919 a         1464 c         1427 g          777 t
ORIGIN      
        1 cgagcgcgga tcgatccgct tgaattccga ccggagagtg gtccgcacca tcactgcgat
       61 cgcgccggcc atctcgacgc cggtgggccc ggcgccgacc aggacgaagg tcagcagatc
      121 gcggtgccgg ctgggatctt cctccgcctc cgcttgctcg aacgcctgca ggatccggtt
      181 ccgaaccgcc tcggcgtctg ccagcgtctt caagccgggc gcgtatggcg cgaactcgtc
      241 gtgcccgaag tagctgtggc tcacgcccgt cgcaaggatc aggtagtcgt agcggatcgg
      301 tacgtccgct cgatccgccg agctggcgac gacgtagcgc tgctccttgt tcactccggt
      361 gacttcgccc atgatcaccg tcgtgttcat ttgcttgccg acgatcccgc ggatcggata
      421 gccgacttgc cccggcgcga gaacggcagt cgccacctgg tagagcagcg gcatgaacac
      481 gtggtgattg ttgcggtcga tcaggatcac ctcgaccggt gcccggcgga gcgccttggc
      541 cgccgccacc ccgccgaagc ctccgccgac aatgacgacg cgtggtagtt gggttggtag
      601 ttgtgcagac atgtccacct caatagaagc cttgggagtt tcgctggacg ctgacctttc
      661 ccacgtagcg ccgcgagatg aagacgaagt aggcaatggc cagcatgaag gccacgggcc
      721 accacatgga cgcaagggcg agcgctctcg ggctggcagc gaccgcttgt gccgtcaggg
      781 agttttccgg agcgagagtc gaatgcagca tcaccggata gagtgccgca cctccggcag
      841 ccaggaggcc ggccagaagg aagttcgagc cgatgaaggc gcgcatttcc cggcgggccg
      901 atagacccga gaacacagtg attgcggagc cgaggacaac cagcactccc agccaccaaa
      961 ccggattggc agccgcgcgg gcggggagat cagggcgaac cacccacgac tcgaacgaga
     1021 tcaggagtag gagcggtacg actgcggccc agagatacct catgtactgc ctgctgcggt
     1081 catgtaccgg gccttcggtc ttcagcgtga ggtaggtcgc gccatgcgct gccagtaaga
     1141 cgaccgtaaa gaccgcgacc gatatcgtat accagtcgag cagtccgacc tgcccgtgca
     1201 cggtgaaatc ggtgaagaac ggcatcgaga agttgccctg ggcgtcgagc ggaacgcccc
     1261 gcgccaggtt acctgccgct gcgccgaaca gggtggccaa cagcaaattg gagcacacga
     1321 agacgaagtc ccagaatcct tgccattggc ggtcgttgat atggccgcct acttcgatcg
     1381 agatcccgcg caggatcagg ccccagagga tcagaaagag cgcgaggtaa tagccggaaa
     1441 aggccgacgc catgagccgg ggaaacactg cgacgaggat accgccaaag ccgaccagcc
     1501 agacctcatg ccaggaccag agtggtccga tggcggcgat gacctggcgg cgttcctcgg
     1561 gtgttttagc gacgagccaa tggagcattc ccgcaccgaa gttgcgccca tccagcacca
     1621 cgtacgcgat gagcatgaac gagacgacgg cgtaccaaat cgggatcata aagtccccac
     1681 cgtttcccga acgggcaccg cccgaccaac ctgcaagtcg cccgggttca cgcgcacgtc
     1741 gtcgtccgga ccttggccga tctcacgacc gaccaggtag agaaacagca ggccgagcac
     1801 gaaatagagg ccgatgaagc caaggagggt gaagatcgtg tcgccgctgc tcacgaccgt
     1861 gctgtggcct tggtttgttc gaaacaggcc atagaccagc cagggctgcc gtcctagctc
     1921 ggccgtcatc catcccagag tgttggctat ataaggaaag ggaaacgcga gcatcagcac
     1981 ccacaggagc ccggtgctgc tttcgagtcg gccgcgccaa ttttggagcc cggcataggc
     2041 catcaggagg atgaacagtg tgccgagcgt aatcatcagg tgaaacgagt agtacaggag
     2101 ctcgatgttg tcgggccagg tcgtctcggg gtattcctga agcccgtgca cgtaactctt
     2161 aaaggtgccg tacgccagaa agctgagtgc gccggggacc tcgatagggt tgtcgaggcg
     2221 gcgggccgcc acattgggtt gcccgatcac cgcgacgccg gccatgggac cacccacgaa
     2281 acgcccttcc atcgcagcga gcgttaccgg ctgatggttg ccgaccatct tggcctgctg
     2341 atctccggtg ggaacggcaa ccagcacgga tgctatcagt ccaactgcgg tacccgcccg
     2401 cagataaagg cgagcctgaa ccggatggcg ccaccgcaac gtgtagaagg ctcccatagc
     2461 cgccaccacg aacgagccgg tgacgagagc cgcaacctga ttgtgcgtga actgaaccaa
     2521 tgcccacggg ttgagcaggt acgagcccag gctgtcgata ctcaaagacc cattggcctc
     2581 gacccgatgg cccaccggat gctgcatgaa ggcgttggtg acgagaatga agaagccgga
     2641 gatccagctc cccagcgcga ctgctactgc ggccaggaaa tgataccgcg gacccagtcg
     2701 cttctcgccc cagatgagcg cgccgatgaa cgcgctctcg aggaagaacg cgaacatact
     2761 ctccattgcc agcgtctggc cgatgacgcc gccggcgtaa cgcgagaagc ccgcccagtt
     2821 ggtgccaaac tgaaattcca tcgggatgcc ggtcacgacc ccgaccgcga aattgagccc
     2881 gaaaatcttg gcccaaaacc gcacggcgtc gttgtacttc tcatccccgg ttctgagcgc
     2941 acgccacttc cagtacacca ggaaccaggc caggcccatg gtgagctgag ggaagaggta
     3001 gtgatacgtc agcgtgaagc cgaactggaa tcggctccaa aacgctggat cggagagtgt
     3061 cacgttcatg gcgtgcgctg cctcgttacc tcccgctctc gtacttgaac gagagcttga
     3121 ccttggcgcg atagctcttg accttgccgt tctcgatggt cacatcgagc tgcacaatct
     3181 cggcgaccct caggtcgcga aggctcttcg acgcctgatt gacggccgcc gcagcagcct
     3241 tctcccacga ctccgagctc gcgcccacca gctcgatcac cttgtatacg ctgtctgcca
     3301 tgactcctcc gtttgtcggc ctcagcttac ggcccgttcc tgatgttccc aaaaccgctc
     3361 ccgcagcacc cgcttcagga tcttcccgga gctgctcttc ggcagatcgg tatcgaagaa
     3421 ttcgactcgg cgggggacct tgtagttggc cagtgactgc cggcagtgcg ccagcagctc
     3481 gtccgcggtg agggtttgtc ccggcttcag caccacgcac gccatgacca gctcccccca
     3541 tttggggtcc gggataccga agaccgccgc ctcgcgcacc gcggggtgct cgtagatcac
     3601 ggcttcgact tcgccggagt agacgttctc gccgccggtc acgatcatgt ccttcagtcg
     3661 atcgaggatg tagaagtacc cgtcgccatc ctgataaccg acgtcgccgg tgcgaaacat
     3721 cccgtctcga aacgccaggg ccgtttctgc cgggttgttc cagtagccgc gcatgacatt
     3781 ggcaccccgg gcgaccagct cgccggtctg acccggtcca agctccttcc ccgtctcgtc
     3841 cgcgacccgc acgtcgatac cgatacaggg gcggccacaa gacgtgaggc gttgctccgt
     3901 gtgctcgtgg tccatcaagc cggtgaggaa cccggtctcg ctcagtccgt agacctggat
     3961 cagcttgatg ccagggaaca gcgtcctggt ccggtggatc agctcgggag ccatgggcga
     4021 gccgccgtag cccagcacct cgagactcgt gaggtcgtac tccgtgatcc ccgggaactg
     4081 cgccagcagg ttgatcatcg tggggaccag cacggtgtgc gtgacccgct cgcgtgcgac
     4141 cacgtcgcag aagccttgcg gggtgaactt cgggatggtg acctggcagg cgccgaagcc
     4201 cggggccgcg aagatgatcg gaaagtccgc aatgtggaag atcggcgccg cgtgcagcca
     4261 ggcgcctccc tcccgatact ccatccaggc gccgaggtgc tcgacggtgg cgaggacgcc
     4321 ggcgtgagtc agcatcacac ccttgggtcg tccggtcgtc ccgctggtat agatgagcgt
     4381 caggatcgcg ttcggatcgt agactggctc aggaggctcg ccgctcccga ggtcaagctc
     4441 ctcctgatcg agcaccacct gccagggaac ctgagtactg ggttgcggca gcgacgagtg
     4501 ccggatcagg ccacgcggat ccgcgtcggc gagcacgcga tcgatctcat gggatgagag
     4561 ccgggtgttc aacggcaccg cgatcac
//
LOCUS       DASRJQ010000456         4439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3047220, whole genome shotgun sequence.
ACCESSION   DASRJQ010000456 DASRJQ010000000
VERSION     DASRJQ010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3047220"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VFB89_10295"
     CDS             complement(<1..445)
                     /locus_tag="VFB89_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27737.1"
                     /translation="MTEIDPVNDDKRDASAELVRRLQRVEHEYHRLRRANRVLLVGLA
                     VLGAASVVTVAVRPAVRDVVEARSFALRDNKGRVRGFFGIAKDGSPQFVLQDDSGAAR
                     LRLTVLGDGSPGVALIDAKGRTRAVLGLLPDETITLAFADQRGTTR"
     gene            complement(532..909)
                     /locus_tag="VFB89_10300"
     CDS             complement(532..909)
                     /locus_tag="VFB89_10300"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HZM27738.1"
                     /translation="MKHVGLTNVTRGLVLGNWIAVADRWWLRLRGLLGRSPLRAGAGL
                     LLRPCRAVHMFGMGYPVDVVFVDRRGSVVAIYHRLGPGQRSRWHKRAACALELPAGTL
                     AMTGTCEGDRLEWESCAPSETSP"
     gene            complement(906..1835)
                     /locus_tag="VFB89_10305"
     CDS             complement(906..1835)
                     /locus_tag="VFB89_10305"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZM27739.1"
                     /translation="MIYFATAAFGIAIGLIVSLVGQLAPARPRAVERLLSELGQRGSN
                     QDALQQRRRRQEQAQRLGALLEWLGGAIARTNRSAVRHVLVHAGYRHPNALAIYCGAR
                     LGLMGALGVSGLLVAPVFGGGMVVGALAALWCAVLGWLAPAVYVGKRARGRQKDIQRA
                     LPDAVDLLVVCVEAGLALNQALVRVAAEIEHVSRVTSEELVLVNLEIRAGVPREEALR
                     SLGERTGVADVRSLVAMLIQTDRFGTSIAQALRVHSDTSRTKRRQLAEEAAAKTTIKL
                     VFPLVLFVFPALFVVILGPAVITMVRTLSQFGR"
     gene            complement(1846..2832)
                     /locus_tag="VFB89_10310"
     CDS             complement(1846..2832)
                     /locus_tag="VFB89_10310"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZM27740.1"
                     /translation="MIQSLGAWAPAGAAFGAGILAVVALALLWEWWAERRRGKDVVEQ
                     LRSFAAQSGTDRASETLMRSAKIVEARWIRPLAARVPHLRDLANTLAQAGVRWSPQTY
                     LLITLGAAVVLGLGALALLRLWVAALVAASGGACLPYLYLQWRKTRFIRAFEEQLPEA
                     IDLLGRAIRAGHPLSAGLKMAAEEATEPIAGELRRVFEEQRFGIPFDDALMGLADRID
                     LVDVRILVTAIMVQREVGGNLAEVLDKIAHTVRARFTIRRQVRVYTAQGRFSGYVLAV
                     LPIAVGCAIFLVNRQYILILIQDHLGRWLLLTGAVLQILGFIWIRRIVNIEI"
     gene            complement(2829..4235)
                     /locus_tag="VFB89_10315"
     CDS             complement(2829..4235)
                     /locus_tag="VFB89_10315"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HZM27741.1"
                     /translation="MTSDSRRSLSAPLPATGGEGQRRSLPWETPVGVDTPGRQAANLQ
                     QIKVRIHRLLIDRLNLSDLDRRERGEVVATIRKVVQDLLTEEHVPLNLEERERLLAQV
                     VDEIFGLGPIESLRQDPEISDILVNTHDQVYIERHGKLEPTEVRFQDDKHLLQVIDRI
                     VSAVGRRIDDSSPMVDARLPDGSRVNAIIPPLAVDGPHLSIRKFRRDALSGEDLVRTK
                     SLTPSALELLRGIVRARLNVLISGGTGAGKTTLLNILSSFIPEDERIVTIEDSAELQL
                     RQPHVVRLETRGANIEGHGEVSQRRLLINSLRMRPDRIIIGEVRGPEAVDMLQAMNTG
                     HDGSLTTLHANGARDALSRLETMVSMANLNLPARAMRQQIASAIDVVIQVSRLSDGTR
                     KLITIAELAGMEGEVITMQEIFTFERQGVDAEGRVIGMFRPTGIRPRFAERLKSWGIT
                     LPADLFSPLAAVSQDGGR"
     gene            complement(4232..>4439)
                     /locus_tag="VFB89_10320"
     CDS             complement(4232..>4439)
                     /locus_tag="VFB89_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27742.1"
                     /translation="YAAVSHAINAGKPVVLNGKSKYTRDLQALGTDLVGVPHPTRAAA
                     RSGSGLFDAFWRRLSQPHVKPSAS"
BASE COUNT          779 a         1549 c         1410 g          701 t
ORIGIN      
        1 cccgggttgt gcctcgctgg tcggcaaacg cgagcgtgat cgtctcatcc ggcagcagcc
       61 cgagcacagc gcgggtgcgt cccttcgcgt cgattagcgc gacgccgggc gaaccgtcgc
      121 caagtacggt cagtcttagc cgggccgcgc cgctgtcgtc ttgaagcaca aactgcggcg
      181 aaccgtcctt tgctatgccg aagaaaccgc ggacccggcc cttattgtct cggagtgcga
      241 agctgcgggc ctccacaaca tctctcacgg ccgggcgcac tgcaacggtg acgacgctcg
      301 cggctccgag caccgccagg ccgacgagaa gaactcggtt ggcgcgacgc agacggtggt
      361 attcatgctc cacgcgctgg agccgccgca cgagctccgc acttgcgtca cgcttgtcgt
      421 cgtttacggg gtcgatttcc gtcattggtc ctccgcctag tcgggcgccg tgaacgtaag
      481 agtgctacgc gggcgcgtcg cagctatcgt ggggatggtg cgctgcttgg attatgggct
      541 cgtttccgat ggagcgcacg actcccactc gagtcgatcg ccctcacacg tgcccgtcat
      601 cgccagcgtg ccagccggaa gctccagggc acacgcagca cgtttgtgcc agcgcgaacg
      661 ctggcccggg cccaggcgat gatatatggc cacgaccgag ccccgccgat ctacgaaaac
      721 gacgtccacc gggtagccca tgccgaacat gtgcaccgcg cggcacggtc gaagcagcag
      781 acccgctccc gcccggagcg gtgaccgacc gagcaaaccc cggagccgca gccaccagcg
      841 gtcggccacg gcgatccagt tccccaggac cagcccgcga gtgacgttgg tgagccccac
      901 gtgcttcatc ggccaaactg gctgagcgtt cggaccatgg taatcaccgc cggtcccaga
      961 atcacgacga acaaggccgg gaacacaaac aggacgaggg ggaacaccag cttaatggtc
     1021 gtcttggccg cggcttcctc ggccagctgg cgtcgcttcg tacgcgacgt gtccgagtgc
     1081 acccggaggg cctgggcgat tgacgtgccg aagcgatcgg tctgaatcag catcgcaacg
     1141 agcgagcgca catccgcgac tccggttcgc tcgccgagcg accgcagcgc ttcctcccgc
     1201 ggcacgcccg cacgaatctc cagattgacc aggacgagtt cctcgctcgt cacccggctc
     1261 acgtgttcga tctccgcagc gacgcgtacc agcgcctggt tgagggcaag ccccgcttcg
     1321 acgcaaacga ccagcaggtc gaccgcgtcc ggcaacgcgc gctggatatc tttctgccgc
     1381 cccctggccc gcttccccac gtagaccgcc ggcgcgagcc atcccagcac cgcacaccac
     1441 agcgcggcca gcgcgccgac gaccataccg ccaccgaaca ccggcgcgac caacaaaccg
     1501 ctgacgccca gcgcgcccat caaccccagc cgagctccac agtagatcgc aagcgcgttg
     1561 gggtgccggt atccggcgtg caccaacacg tggcgcacag cggaacggtt ggtccttgcg
     1621 atcgcgccac ccaaccactc cagcagggcc ccgagccgtt gggcctgctc ctgccgtcgc
     1681 cgccgctgtt ggagcgcgtc ttggttgctc ccccgctgcc cgagttccga taacagacgc
     1741 tccaccgccc gtggccgcgc gggcgcgagc tgtccgacca gggaaacgat cagcccgatg
     1801 gcgatcccga acgccgcggt cgcgaaatag atcatctgaa ggtcctcaga tctcgatgtt
     1861 cacgatgcga cggatccaga tgaagccaag aatctgcaac accgctccgg tgagaagcag
     1921 ccaccggccc aggtgatcct ggatcaggat caggatgtat tgtcggttga ccaggaagat
     1981 cgcgcagccg acggcgatgg ggagcaccgc caggacatag ccgctgaagc gcccctgggc
     2041 ggtgtagacc cgcacctgac gtcgaatcgt gaagcgtgcg cggacggtat gcgcaatctt
     2101 atcgagcacc tcggcgaggt tgccaccgac ctcccgctgc accatgatgg ccgtcaccag
     2161 gatccggacg tcgaccaggt cgatccggtc cgccagtccc atcagggcat catcgaacgg
     2221 aatgccgaag cgttgttctt cgaacacgcg acggagctcg ccggcgatcg gctccgtggc
     2281 ctcctccgcg gccatcttga ggcccgccga cagcggatgg ccggcccgga ttgcgcggcc
     2341 gaggaggtcg atcgcctcgg gcagctgctc ctcgaaggcc cggatgaagc gcgtctttct
     2401 ccactgcaga tagaggtaag gcaagcacgc gccaccgctc gccgcgacga gcgctgccac
     2461 ccagagccgc agaagggcga gtgccccgag ccccagcaca accgcggcgc caagcgtgat
     2521 caggagatag gtctggggac tccaccgcac tcccgcctgc gccagcgtat tcgcgagatc
     2581 gcgcaggtgg ggcactctgg cggcgagtgg ccggatccac cgcgcttcca cgatcttcgc
     2641 actgcgcatc agcgtctccg aggcccgatc ggtgccgctc tgtgccgcga acgagcgcag
     2701 ctgctctacg acgtcttttc cccgccgccg ttccgcccac cactcccaga gcagcgcgag
     2761 tgccacaacg gccaggatgc ccgcgccgaa ggccgcgccc gcgggggccc aggcgcccag
     2821 cgactggatc atcgcccacc atcctgcgat accgccgcaa gcggcgagaa caggtccgcc
     2881 ggcagcgtga tgccccagga cttgagccgt tcagcgaagc gcggccgaat gccggtcggg
     2941 cggaacatcc ctatcacgcg gccttcggcg tcgacgccct ggcgctcgaa ggtgaagatc
     3001 tcctgcatcg tgatgacctc accttccatc cccgcgagct cggcgatggt gatcagcttg
     3061 cgagtcccat ccgagaggcg gctcacctga atgacgacgt cgatcgcact ggcgatctgc
     3121 tggcgcatcg ctcgtgcggg caggttgaga ttcgccatgg aaaccatcgt ctcgagacga
     3181 ctgagggcgt cgcgcgcacc gttggcgtgc agggtcgtca gcgaaccgtc gtggccggtg
     3241 ttcatcgcct gcagcatatc gaccgcctca ggaccccgga cctcgccgat gatgatgcga
     3301 tccggccgca tacgcagcga attgatgagg agccgccgtt gcgacacctc tccgtggcct
     3361 tcgatgttgg cacccctggt ctcgaggcgc accacgtggg gctggcgcag ctgcagctcg
     3421 gccgagtcct caatcgtcac gatgcgctcg tcctccggaa tgaacgagga caggatgttg
     3481 agcagcgtgg tcttaccggc gcctgtgcct ccagaaatca ggacgttcaa tcgcgcccgc
     3541 acgatgccgc gcagcagctc cagcgccgac ggcgtcaggc tcttcgtgcg gaccagatcc
     3601 tcgccggaca gggcgtcgcg tcggaacttc cggatggaca gatgcggccc gtcgactgcg
     3661 agggggggga tgatcgcgtt gacccgcgac ccgtctggca agcgcgcgtc gaccatgggc
     3721 gaggaatcgt cgatgcgccg cccgaccgcc gagacgatcc gatcgatcac ctgcaacaag
     3781 tgcttgtcgt cctggaaccg cacctcggtg ggctcgagct tgccgtggcg ttcgatgtac
     3841 acctggtcat gcgtgttgac gagaatgtcc gagatctcgg gatcctgcct caaggactcg
     3901 atgggcccga gcccgaagat ctcgtcaacg acttgcgcca gcagccgctc ccgctcctcc
     3961 aagttgagcg gtacatgctc ctccgtgagg aggtcctgga cgaccttgcg gattgtggcc
     4021 accacctctc cccgctcgcg ccggtcgagg tcggagaggt tcaggcggtc gatcagcagg
     4081 cggtggatcc gcaccttgat ctgctggagg ttggctgcct gtcgccccgg agtgtcgacg
     4141 ccgaccggtg tctcccaggg aagcgagcgg cgctgcccct cgccacctgt ggccgggagc
     4201 ggcgcgctca acgaacggcg cgagtcgctt gtcatgaagc gctcggtttc acgtgaggct
     4261 ggctgaggcg ccgccagaag gcgtcgaaga ggccgctgcc acttcgggcg gcagcgcggg
     4321 tcggatgcgg cacacccaca aggtccgtgc ccagcgcttg aaggtcacgc gtgtacttgg
     4381 acttgccgtt caacaccacc ggcttgccgg cattgatcgc gtgactcacc gccgcgtag
//
LOCUS       DASRJQ010000457         2712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_267870, whole genome shotgun sequence.
ACCESSION   DASRJQ010000457 DASRJQ010000000
VERSION     DASRJQ010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267870"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1466)
                     /locus_tag="VFB89_10325"
     CDS             complement(<1..1466)
                     /locus_tag="VFB89_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27743.1"
                     /translation="MIAFTLTVVLFFFWLVVGSAVVAALSTRRSVLQGLLLAPAVGIA
                     MTVLPIFLLNQLGLPVRTFGIPLTVVLLAASLGVLWSRRPLWSARRCLPLLRVYLPYF
                     AVFVASILLTGWPMLRFGLDWVSYANDDMANYVLLAHRHLDHAYFDIPTNDALLQGTD
                     YAQFYWIFVVIGERSGAQLILAWVISMTGWTGLQVYMPFILAMNAGLVSAAAALAYQS
                     GRAKLAAFMTAILLAMSSLNSLGALFQLSAQVGGVALLVSAATLLLRPIGPMSLVAAV
                     RHGLLVAIVGSALLVFYPEVTPFLGLSFGLYVLVVLLRRRSTVSFRSASVALGVAAGV
                     GLAVVNLYAFTAINFLARQAVTATRPKTEVDIDQALFPYFLIPSGLANLWGFQPITYL
                     TGEPWLSMSVIVGALLLVAVTAAALVLAWRAQPAAIVTVVMLLLSAQLVRQGVGFGLF
                     KLAMFAQPFLLGVLVAGWFLVTRRTPMRVGPLILVGMLG"
     gene            1898..>2712
                     /locus_tag="VFB89_10330"
     CDS             1898..>2712
                     /locus_tag="VFB89_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27744.1"
                     /translation="MTVLLIFAISFSFNVLTRDNLLGPQFDAWLGVWGVRLQTFASIA
                     QVVTLLAWTGWIFRRQAVPVYFSDTVASLRTTATFRLLGKAPIALLVRATTWLAASVA
                     IVWLYAWMASGGTFRFDSASTPYADMLADAFLAGQLNLLVEPSPALLALPDPYDAVMN
                     KPFRLPDASLFNGHYYLYFGPVPGLIHAAWKLLTGSRLEEGAMLMVAGVAGCFWFLLV
                     VRRLRRLTFPELSPAWERAAAVCYALGGVGLFLVSRPIGHHESILVASAFVLGG"
BASE COUNT          540 a          845 c          888 g          439 t
ORIGIN      
        1 ccaagcatgc cgaccaggat cagcggaccc acacgcatcg gagtccggcg cgtgaccagg
       61 aaccagccag caacgagtac gcccagcagg aacggctggg cgaacatcgc gagcttgaac
      121 aggccgaatc cgacgccctg cctgaccagt tgtgccgaga ggaggagcat gaccacggtg
      181 acgatcgccg cgggctgagc gcgccaggcg aggacgagcg ctgctgccgt gacggcgaca
      241 agcaagagtg ctcccacgat cacactcatg gagagccagg gctcgcccgt gagataggta
      301 atcggctgga agccccacag attggccaga ccgctcggga tcaggaagta cggaaacaag
      361 gcctggtcaa tatcgacctc ggttttcggt cttgtggcgg tgacggcctg cctcgccaga
      421 aagttgatcg ccgtgaaggc gtacaggttg acgacggcca ggccgacacc agccgccacc
      481 ccgagcgcga cgctcgcgga acggaacgat accgtcgatc tacgccggag cagcacgacg
      541 agaacgtaga gcccgaaaga cagacccagg aacggcgtca cctctgggta gaatacgagg
      601 agtgccgatc cgacgatcgc caccagcaac ccgtgtcgga cggctgcgac gaggctcatc
      661 gggccgatcg gacgcaggag cagggtcgcc gcgctcacca ggagagccac cccacccacc
      721 tgcgccgaca attgaaacag ggcgccgaga ctgttgagcg acgacatggc gagcaggatc
      781 gccgtcatga aggcggcgag ctttgcccga ccggactggt aagccagggc agccgcggca
      841 ctaacgagcc ctgcattcat cgccaggatg aatggcatgt atacctggag gccggtccag
      901 cccgtcatgc tgatgaccca ggccagaatg agctgggctc cggatcgctc gccgatcacg
      961 acgaagatcc agtagaactg ggcgtagtct gtaccctgaa gcagggcgtc gttggttggg
     1021 atgtcgaagt aggcgtgatc gagatggcga tgggcgagga gcacgtagtt ggccatgtcg
     1081 tcgttcgcgt agctgaccca gtcgagccca aaccggagca tcggccagcc ggtcagcagg
     1141 attgacgcga caaatactgc gaaatagggc agatacacgc gcaggagcgg gaggcatcgt
     1201 cgagccgacc agaggggacg ccgcgaccag aggactccca gactcgcagc gagaagcacc
     1261 accgtgagcg gaatgccaaa cgttcggacg ggcagcccga gctgattcag gaggaagatc
     1321 ggcagcaccg tcatcgcgat cccgaccgct ggtgcgagca agaggccctg gaggacgctt
     1381 cgtcgcgtgg atagcgcggc gactaccgcg gagccgacca ccagccagaa gaagaagaga
     1441 accaccgtga gcgtgaacgc gatcaacgac tcctccgcaa cggcggcaga ttatacggga
     1501 caggctaaat ggctgagaac cgcgcgccat gaacgggcga ggttccaggc gttgggactg
     1561 ccatgccttg tcacaacaag cgacgagcct ggatacttgg gccgagcttg atcagcccag
     1621 gagcgacgaa gaggcgcctg gttccgaacg cacgatgact gagcccgaac tcgagcgttc
     1681 cggggaggct ggagcctcga ctctacgatg caatgaagga cgaattgtcg gcaccatgac
     1741 cactgagcta cgtgtgccct cgacgtctcc tgagagcgat gagggttcca gaatcatggt
     1801 cacgaacact gacccgcgca gacttgttgg ggactcggac gaagacccgc gggccccgac
     1861 tgttctgtgt ctcgcatgcg cccgatcgcg cttctcgttg acggtcttgc tcatatttgc
     1921 gatctcgttc agcttcaatg tcctgacccg cgacaatctc ctcggcccgc aatttgacgc
     1981 gtggctcgga gtttggggcg tacgacttca gacgttcgcc agcatcgccc aggtcgtgac
     2041 gctcctggca tggactggct ggatcttccg ccggcaggca gtcccggtct acttcagtga
     2101 cacggtcgcg agcctgcgga cgacggccac gttccgtctg ttggggaagg cacccatcgc
     2161 gttgctggtg cgcgcgacga cgtggctcgc ggcctctgtc gccatcgtct ggctgtacgc
     2221 ctggatggcc tccggtggga cgttccgctt cgacagcgcc tcgacgccgt acgccgacat
     2281 gctcgccgac gcgttcctgg ccggccagct caatctgctc gtcgagccgt cgccggcgct
     2341 gttggcgctg cctgacccat acgatgccgt gatgaacaag ccgtttcgcc tgccggacgc
     2401 atcgctattc aacggccatt actacctgta cttcggaccc gtgccgggcc tgatccatgc
     2461 ggcgtggaag ctgctgaccg gatcgcgcct cgaggaggga gcgatgctca tggtagccgg
     2521 ggtcgccggc tgcttctggt tcctactcgt cgtacggcgc cttcgacggc tcacgtttcc
     2581 tgagctctcg ccggcgtggg agcgcgccgc ggccgtctgc tacgccctcg ggggtgtcgg
     2641 ccttttcctg gtatccaggc cgatcggcca ccacgagtcg atcctggtag cgagcgcgtt
     2701 cgtgctgggt gg
//
LOCUS       DASRJQ010000458         2683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2740498, whole genome shotgun sequence.
ACCESSION   DASRJQ010000458 DASRJQ010000000
VERSION     DASRJQ010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2740498"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VFB89_10335"
     CDS             <1..522
                     /locus_tag="VFB89_10335"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HZM27745.1"
                     /translation="LHGLGWEFVGGEQVPWPSSLARGPYGWAQVATFVVTGLLIVILA
                     VAVRDQLPQRRASGFAVVLLAMLGVALILAGFRVDVPMLSGGTPATWNGCVHGIAFLL
                     IIATGVLAPLTMALAVRRDPGWRPIAVVSVATSALVVVFLFLPWGSATFLLAIITLFG
                     WIAWVAVRLATHS"
     gene            complement(706..1941)
                     /locus_tag="VFB89_10340"
     CDS             complement(706..1941)
                     /locus_tag="VFB89_10340"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZM27746.1"
                     /translation="MRSIVRFLDRLSDLGAGPEDSEDERLRHGTLIFASMVITLISLT
                     WVATYLAYGYPTSAAIPALYQLITVVGLIVLARTRRFGVFRTTQLLAFLVLPALLQAS
                     LGGFVASSAMILWAIFTPLAALALQGLRRSVVWFVAFLAELVVLGLLDPRLSQDPAAL
                     PTGLVITFFVLNVTGLTLSAYVMLGYFVEQRERARRALEAERERSERLLLNVLPGPIA
                     ERLKVGTGVIADHYDTVSVLFADLVGFTERSLVLAADELVALLDEIFSAFDRLADAEG
                     VEKIKTIGDAYMVAGGLPEPRPDHLEAVARMALAMRDEIAAIAERTGRDWLAVRIGID
                     TGPVVAGVIGRRKFIYDLWGDTVNTASRMESHGLPGEIQVTDRVAAALGPGFAVRPRG
                     TRDLKGKGPMATFLLGGAH"
     gene            complement(2200..>2683)
                     /locus_tag="VFB89_10345"
     CDS             complement(2200..>2683)
                     /locus_tag="VFB89_10345"
                     /inference="COORDINATES: protein motif:HMM:NF044515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZM27747.1"
                     /translation="GIGRATLYKYFPDVEMILSAWHQRHVEAHLAELRRIQERTADPV
                     TRLQAVLARYADICRKRRRHGDDELVAVLHRSTQVRKLQRQLLDLISGLVADAAGAGA
                     IRQDVPAEELASYCVHALAAAGDSSTTAIDRLLDVVWTGITSTRCTQSHVRHSASQPD
                     "
BASE COUNT          450 a          859 c          890 g          484 t
ORIGIN      
        1 cttcatgggc ttggttggga attcgtgggc ggcgagcagg ttccgtggcc tagctcgctg
       61 gcgcgcgggc cgtacggttg ggcgcaggtt gcgacctttg ttgtcaccgg cctgctcatt
      121 gtgatcctcg cggtggcggt gcgcgatcag ctgccgcaga ggcgggcaag tggtttcgcg
      181 gtggtgctgc tcgccatgtt gggggtggcg ttgatcttgg ccgggtttcg ggttgatgtg
      241 ccgatgctga gtggcggaac tcccgccacc tggaacggct gtgtgcacgg gatcgcgttt
      301 ctgttgatca ttgcgaccgg ggtgctcgcg ccactgacga tggcgctcgc ggtgcggcgt
      361 gatcccggct ggcggccgat cgctgtcgtt tccgtcgcga ccagtgcgct cgtcgtcgtc
      421 ttcttgtttt tgccgtgggg cagcgccaca ttcctgctgg cgatcatcac cctcttcggc
      481 tggattgcct gggtcgcggt acgcctagcg acgcactcct aaaaccatcc tgtgcttatg
      541 tggccgatga agatggcgct ctgacagccg ccgcgctttg agaatccgga agtgtgatgc
      601 agtgcttgcc gtttgcggca gcgtctggga ggagcacctc aggattaagt gattgggtgg
      661 cgcttagctc gtgttcgcat gccatgcggc gatgccgaac gtcagctagt gggcgccgcc
      721 aagtaggaac gtcgccatgg gccccttccc cttcaggtcc ctggttccgc gtggccgcac
      781 ggcgaacccg gggccgagcg cggccgcgac gcggtcggtg acctggatct ctccgggcag
      841 cccgtgggac tccatccggc tggccgtgtt cacggtgtcg ccccagaggt cgtagatgaa
      901 tttgcggcgg ccgatcacgc ccgcaaccac cggtccggtg tcgatcccga tccgaacggc
      961 gagccagtcc cgtccggtcc gctccgcgat cgccgcgatc tcatcccgca tcgcgagtgc
     1021 catgcgggcg accgcctcga ggtggtccgg gcgtggctct ggcaacccgc ccgccaccat
     1081 gtaggcgtcg ccgatcgtct tgatcttctc gaccccctct gcatcggcga gccggtcgaa
     1141 cgccgagaaa atctcatcca ggagggccac cagctcgtcg gcggccagga ccaacgaccg
     1201 ctcggtgaag ccgacgaggt ccgcgaagag cacgctcacc gtgtcgtagt gatccgcgat
     1261 caccccggta ccgaccttca ggcgctcggc gatcggcccg ggcaagacgt tcaacaagag
     1321 gcgctccgac cgctcccgct ccgcctccag cgcacgacgc gcccgctccc gctgttccac
     1381 gaaatacccg agcatcacat aagcgctcaa ggtcagcccc gtaacgttca gcacgaagaa
     1441 cgtgatcacc aagcccgtcg ggagcgccgc cgggtcctgg gacaggcgag ggtcgagcag
     1501 cccgagcacc accagctcgg cgaggaaagc cacaaaccac accactgagc gccgcagccc
     1561 ctgtagggcg agcgccgcga gcggtgtgaa gatcgcccac aagatcatcg cgctcgatgc
     1621 gacgaagccg ccaagcgagg cctggagcag ggcaggcagc acgaggaacg cgaggagctg
     1681 cgtggtccga aacactccaa accgtcgggt ccgcgccaac acgattagcc cgaccaccgt
     1741 gatcagctgg tatagggccg ggatcgccgc agacgtgggg tagccgtagg cgaggtatgt
     1801 agctacccag gtcagtgaga tcagcgtgat caccatcgac gcgaagatca gggtcccatg
     1861 gcggaggcgc tcatcttcag aatcctccgg tccggcgcca agatcggaca gtcgatcaag
     1921 gaagcgcacg attgaccgca tcgatccgcg cgctggagaa ccgacaagga catgcgatgc
     1981 ggccacgccg ccgatgctag cttggccacc accggaaccc tccgacatcg tgcttcggca
     2041 ctggccccag cgcggcggct cggacacgtt tgagacttac tgatcatctt ttggcaaccc
     2101 aggctggtga catcagagcg aactggcggc gcgagggtaa cggaactgcc aggctagacg
     2161 tgccggccgc tcggtgtcgc gaaggcagct gtctgtcgtt cagtcgggtt ggctcgcact
     2221 gtgccggacg tggctctgag tacagcgcgt cgaggtgatt ccggtccaga cgacgtcgag
     2281 cagtcgatcg atggccgtgg tggacgagtc gccagccgcg gcgagggcgt gaacgcaata
     2341 gctggccagt tcttcggcag gaacatcttg tcgtatcgcg cccgctccgg ctgcgtcagc
     2401 caccaagccg gagatcaggt cgaggagctg ccgctgcaat ttgcgaacct gggtgctgcg
     2461 atgcagcacc gccacgagct cgtcgtcgcc gtggcggcgc cgcttccgac agatgtcggc
     2521 gtaccttgcc agtacggctt gcagacgagt aactggatcg gctgtccgct cctggatccg
     2581 tcgtagttcc gccaggtggg cctcgacatg gcgctgatgc caggcgctca agatcatttc
     2641 cacatcgggg aagtatttgt agagcgtcgc ccggcctatt cct
//
LOCUS       DASRJQ010000459         5039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3530769, whole genome shotgun sequence.
ACCESSION   DASRJQ010000459 DASRJQ010000000
VERSION     DASRJQ010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3530769"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VFB89_10350"
     CDS             complement(<1..828)
                     /locus_tag="VFB89_10350"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZM27748.1"
                     /translation="MDNDFVTRERDRLLSELGDFLSIPSVSALPEHAADCRVAAQWLA
                     AELGRLGCAHVTLLEGRGHPVVWAESPPVEGKPTLLVYGHYDVQPPDPLEEWESPPFS
                     PTVRDGNLFARGADDDKGQVYCLLKAYEAVRAPDGKPPLNVRFIFEGEEECGGHVISD
                     LLREEPWRTKVDAALISDMAYYAPGIPAVYTALRGICYAEIVVRTLDQDLHSGSFGGV
                     APNAIETLVRMLSELKDESGRIQIPKLYKDVKSPSKSELKAWKRLPFEKRQYLETEVG
                     "
     gene            986..1273
                     /locus_tag="VFB89_10355"
     CDS             986..1273
                     /locus_tag="VFB89_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27749.1"
                     /translation="MHRSLATLAGAAALALVTVTASAQQQPAAPAPQQNPPVETGALD
                     TTKTMTPAPTTPSATTDTGYTAAKDTSADSSKSKKKGKYGEPTDTTKPKQD"
     gene            complement(1347..2453)
                     /locus_tag="VFB89_10360"
     CDS             complement(1347..2453)
                     /locus_tag="VFB89_10360"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HZM27750.1"
                     /translation="MARGVMIFFIVVGVLMAGYGTAYIASEDVRYLTRAGMEETRILQ
                     SRRPIADLVRNPATKPELRSQLQLVLDTRDYAAQLGLAAKQTYTTYSDVGRDTLLLNL
                     SAAPKDCICPYTWKYPIVGRIPYKGFFDVAAGKREADRLAARGYDVYLRPAAAFSTLG
                     WFNDPLLSTALTEDSVELAALVFHEIAHNSLYVRSATPFNESFAQLVAYRAAEHFFRA
                     RGDSIASQRAADRWHDEIVLGEYYDSLVSRLNRLYDSHPSPETLDSGRSAAGAWARAQ
                     LEGPIGSQLKTFRIGRLAERPVNNARLVGARIYRTRLDLFDRWYDAHGQDVGKSVAAL
                     RDLVGDTEGDSALGRLARAVGDSVRAGVDSTAGK"
     gene            complement(2462..4201)
                     /locus_tag="VFB89_10365"
     CDS             complement(2462..4201)
                     /locus_tag="VFB89_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27751.1"
                     /translation="MSHKLALVSILSVASLAACGNDSSGPNPPPSLNCNQVTPTALSP
                     GEASVTDPTATSPCVAVPSVPAAGSSGAEYLYVAAATAGQQTNSGITGQYAITGASLT
                     TAVADESPLPSPLLSAFKPPLTAEKFHAMLRQREKQTARDPRTAFRAKVAHPSLAAAP
                     PPDSGAQRTFLVCRNANCDSPFVPVTATAQHVGAKVAIFVDNAASATQRYSATDLQNV
                     GRLFDNFLYPIDTTNFGRESDIDGNGVVYVLLSPQINKLSGNCNSTNSVILGYFFSID
                     LEPGQTGSNAAEIFYSIVPDPVSPICTLSKSFALDNLPPTFIHEFQHMISYGQHALTL
                     NGDAEDTWLNEGLSHYAEELGGRLIPDDQNPTLGRSPNTLTQFAFANYDNAYKYLSNP
                     EITALIESAQSSGSLTERGANWLMVRWLTEQFGTSTEDASQFIVKGTDFTLKLERTTR
                     VGADNVAQQSGQSFSDLDTYWNLANYLDHLPGFTPADPRLGYLSLNLRSKFAQLNQQD
                     PDTYPRPYPLAPDSTTTGTYQRAGTLKQGSGHHVRIKQGPGAAAVQFRLAGTNGAAIS
                     SDLVPRIGLVRIR"
     gene            4306..4959
                     /gene="hisIE"
                     /locus_tag="VFB89_10370"
     CDS             4306..4959
                     /gene="hisIE"
                     /locus_tag="VFB89_10370"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF002747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HZM27752.1"
                     /translation="MSDEAWLAHIKFDAEGLVPVVAQESRSGDVLMVAYADREALKQT
                     RASGLAHYFSRSRRTLWQKGATSGHIQRVREIRLDCDGDTVLYRVDASGPACHTGERT
                     CFAWQVTNGGDTAHTVDPGAHPISRLARRIHDRATTRPPGSYTAKLFEGGPTAVAQKV
                     GEEATEVVVAALAQDGGSLTSEAADLLYHLLVLLEIKGVTVDDVWRELEAREKQRER"
BASE COUNT          965 a         1555 c         1599 g          920 t
ORIGIN      
        1 gccgacttcg gtttccagat actgacgctt ttcgaacggc agccgcttcc acgccttgag
       61 ctccgacttg ctgggcgact tcacgtcctt gtacaacttc gggatctgaa tccggccgct
      121 ctcgtccttg agctcgctga gcatgcggac gagcgtctca atcgcattgg gcgccacccc
      181 gccgaagctg cccgagtgca ggtcctggtc gagcgtccgc accacgatct cggcgtagca
      241 aattccccgg agcgcagtgt agaccgccgg tatgccgggc gcgtagtacg ccatatcgct
      301 tatcagcgcg gcgtctacct tggttcgcca cggctcctct cgcaacaaat cggagatgac
      361 gtgcccgccg cactcctctt cgccttcgaa gatgaatcga acgttgagcg ggggcttgcc
      421 gtccggcgcg cggacggcct cgtacgcctt gagcaggcag taaacctggc ccttgtcgtc
      481 gtcggcgccg cgggcgaaca gatttccgtc ccgtacagtg ggcgagaacg gaggcgactc
      541 ccattcttcg agcgggtcgg gcggctgaac gtcgtagtgg ccgtagacca gaagggtcgg
      601 ctttccttcg accggtgggc tctccgccca gacaaccggg tggcctcgac cctcgagcag
      661 cgtgacgtgc gcgcagccca aacgccccaa ctcggcggcc aaccattggg ctgcgaccct
      721 acaatccgca gcatgttcgg gaagtgcgct cacgctggga atggacagaa agtccccgag
      781 ctcgctgagc aagcgatccc gttcccgagt tacgaagtcg ttgtccatac ttaagtaaag
      841 tacaggtata agccgaaggt gagaagccgc aatcaatgtg atttaaatca cagcggggac
      901 ttttttgggg gtgtagcctc tccgggcccg gtcgccgtcg ggtcccggcg gcttccacag
      961 actatatcat tttcggaggt aataaatgca tcgcagcctt gccacgcttg ctggagccgc
     1021 cgcattggcg ctcgtaaccg tgacggcctc agctcagcag cagcccgccg ccccggcgcc
     1081 ccagcagaat cccccggtcg agactggtgc gttggatacc accaagacca tgacgcctgc
     1141 tccgacgacg ccctctgcga cgaccgacac cggttacacc gcagcgaagg acactagcgc
     1201 cgattcctcc aagagcaaga agaaggggaa gtacggcgag ccgaccgaca cgacgaagcc
     1261 gaagcaggac tgagtcgagt aggtcttcaa taaaagacga gaccgggtga ggggcgtgag
     1321 ggaaggtcct cgcgtcactc acccgttcac tttccggcgg tcgaatcaac cccagctcgc
     1381 accgagtcac ccacagcgcg agccaatctt cccagcgcac tatctccctc ggtatctcct
     1441 acgaggtccc gtagcgctgc cacgctcttg ccgacgtcct gtccatgcgc gtcgtaccac
     1501 cggtcgaaaa gatccagccg ggtacggtag atccgcgcgc ctaccagccg ggcattgttc
     1561 acgggacgct cggccagacg cccgatgcga aacgtcttga gctgggatcc gatcgggccc
     1621 tcgagctgtg ccctcgccca agcccctgcc gcagagcgtc ccgagtcgag tgtttccggt
     1681 gagggatgcg aatcgtaaag tcggttcagt ctgctgacca gagaatcgta atactcaccc
     1741 agaacgattt cgtcgtgcca gcgatcggcc gcacgctgcg acgcgattga atcgcctctt
     1801 gcccgaaaga agtgctctgc cgcccggtac gcgaccagct gtgcaaagct ctcgttgaag
     1861 ggcgtcgcgc tccgcacgta cagagagttg tgcgcgatct cgtgaaagac cagtgcggcg
     1921 agctcgaccg aatcttccgt cagtgcggtg gagagcagtg gatcgttgaa ccaaccgagc
     1981 gtggagaagg ccgcggccgg acggaggtag acgtcgtatc cccgcgccgc gagtcggtca
     2041 gcctcgcgct tccccgcggc aacgtcgaag aatcccttgt atggaatccg ccccacgatg
     2101 ggatatttcc aggtgtaggg gcagatacag tccttgggcg cggccgacag attgagcagc
     2161 agcgtatccc gaccgacatc cgagtaagtc gtataggtct gcttggcggc gaggcccagc
     2221 tgcgcggcgt agtcgcgagt atcgagaacc agctggagct gactccgaag ctccggcttg
     2281 gtggcgggat tccggaccag atcagcaatg ggccggcgag actggaggat tcgagtttcc
     2341 tccatgccgg cccgggtgag gtagcggacg tcctcgctcg cgatgtaggc cgtgccatac
     2401 ccggccatca ggacgcccac aacgatgaaa aagatcatga cgccgcgggc catccctcgg
     2461 gtcagcggat ccgcaccagg ccgatccgcg gaacgaggtc gctcgaaatt gcggcgccgt
     2521 ttgtacctgc caaacggaac tgcactgcgg ccgctcccgg cccctgtttg atcctcacgt
     2581 gatgacccga cccctgtttc aaggtgccgg cccgttggta ggtcccggtc gtcgtgctgt
     2641 ccggcgcgag cgggtacggg cgcgggtaag tgtccgggtc ctgctgattc agctgagcaa
     2701 atttggaccg caggttgagg ctgaggtaac ccagtcgggg gtcggccggc gtgaagcccg
     2761 gcaggtgatc caggtagttc gccaggttcc agtaggtatc cagatcggag aacgactgac
     2821 ccgactgttg ggccacgttg tccgcgccaa cacgcgtggt ccgctccagc ttgagcgtga
     2881 aatccgtgcc cttgactatg aactggctcg cgtcctccgt cgaggtgccg aactgctccg
     2941 tcagccaacg caccatcagc cagttggcgc cgcgctccgt caacgaccca ctcgactgcg
     3001 ccgactcgat cagcgcagtg atttccggat tcgacagata cttgtacgca ttgtcgtagt
     3061 tggcgaacgc aaattgggtc agcgtattag gtgatcgacc cagagtcgga ttctgatcgt
     3121 cagggatcag ccgcccaccc agctcttcgg catagtgaga gagtccctcg ttcagccagg
     3181 tgtcttccgc gtccccgttc agcgtcaggg cgtgctggcc gtagctgatc atgtgctgaa
     3241 attcgtggat gaacgtcggc ggcagattgt ccagcgcgaa cgacttcgac agcgtgcaaa
     3301 tggggctcac cggatcggga acgatgctgt aaaagatctc ggccgcgttc gagccggtct
     3361 gaccgggctc cagatcgatg ctgaagaagt agcccagaat cacgctgttg gtgctgttgc
     3421 agttcccgct cagcttgttg atctgcgggg agagcagcac gtatacgacg ccattgccgt
     3481 cgatgtcgga ttcgcgaccg aaattcgtgg tatcgatcgg gtagagaaaa ttgtcgaaca
     3541 gcctgccgac gttctggagg tcggtcgcgc tgtaacgttg tgtcgcgctc gcagcgttgt
     3601 cgacgaagat cgctaccttg gccccgacgt gctgcgcggt tgccgtgacc ggcacgaacg
     3661 gcgagtcaca gttggcgttc cggcacacaa gaaacgtcct ctgggctccc gaatcgggcg
     3721 gcggtgccgc cgcgagcgag gggtgcgcca ccttcgccct gaaagcggtg cgaggatcgc
     3781 gggccgtctg cttttcgcgc tggcgcagca tcgcgtggaa cttctcagcc gtgagtgggg
     3841 gcttgaaggc actcagcagc ggcgagggca gcgggctctc gtccgcgacc gcagtggtga
     3901 gcgacgcacc ggtgatggcg tattgacccg taattcccga gttggtctgc tgtccggcgg
     3961 tcgccgcggc cacatagagg tattcggcgc cgctgcttcc agcggccggc actgacggca
     4021 ccgcgacaca ggggctggtc gcggtcggat cggtgacgct ggcctccccg ggggaaagcg
     4081 ccgtgggagt tacctggttg cagttgagcg acggaggggg attcggacca ctcgagtcgt
     4141 taccgcaggc agccaaggac gccaccgaaa gaatcgaaac aagcgcaagc ttatgggaca
     4201 cgttagaagg tgctccggca gggtgagttg accgggctag aaccgagcaa gatacgacag
     4261 gacaacagct ttgacaatcg ccggaggggt ccgtaccttg acaccgtgtc cgacgaagcc
     4321 tggctggctc acatcaaatt cgatgccgaa gggctggtcc ccgtggtggc gcaggagagc
     4381 cgctccgggg acgttctcat ggtcgcctac gcagaccggg aggccctcaa acagacccgc
     4441 gctagtggtc tggctcacta tttcagccgg tcccggcgga cgctgtggca aaaaggcgcg
     4501 acatcgggcc atatccaacg agtccgagaa attcgactcg attgtgacgg ggacacggtg
     4561 ttgtatcgtg tcgatgcatc ggggcccgcc tgccacaccg gggaacgtac ctgtttcgcc
     4621 tggcaggtga cgaacggcgg ggacacggcg cacacggtcg atcccggtgc gcatccgatc
     4681 agtcggctcg cccggcgcat tcatgaccgc gccaccactc gtcctcccgg gtcctacaca
     4741 gcgaagctgt tcgagggcgg cccgaccgcc gttgcgcaga aggtcggcga ggaagcgacc
     4801 gaggtggtcg tcgcggcatt agcccaagac ggcggcagcc tcacctcgga ggccgccgac
     4861 ttgttgtatc acctgctcgt gctgctggaa atcaaaggcg tgacggttga cgatgtgtgg
     4921 cgggaactgg aggcaaggga gaaacagcgt gaacggtaaa cgctcttaga aaaaaatggc
     4981 tttcgttcac ctccacaccc acagcgaata ctccctgctc gacggcgcaa atcggatca
//
LOCUS       DASRJQ010000460         2061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2846815, whole genome shotgun sequence.
ACCESSION   DASRJQ010000460 DASRJQ010000000
VERSION     DASRJQ010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2846815"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VFB89_10375"
     CDS             complement(<1..304)
                     /locus_tag="VFB89_10375"
                     /inference="COORDINATES: protein motif:HMM:NF024602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HZM27753.1"
                     /translation="MNPARRACSLAGLLVATILVLAASAVNAAVVYPVKVGPTGRYLV
                     DQSNVPFLMAGESPQAMIGDLTEAEAEQFFENRRGHGFNTVWINLLCNGYTGCAGDG"
     gene            complement(515..>2061)
                     /locus_tag="VFB89_10380"
     CDS             complement(515..>2061)
                     /locus_tag="VFB89_10380"
                     /inference="COORDINATES: protein motif:HMM:NF025418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HZM27754.1"
                     /translation="NIPLKNWGGFSLHQDAVYDDLERLVTAGLADRTLLNTKPISRVE
                     AARIVARAIERIRRDEGGLNERRDLEPVLDRLIEEFKPELAGLGVQVPDAVAAPGTFS
                     FTPVDRAMAFAGYASRRLSLVNEQGGSFQKWFDSGLTFESRLQIGDFLTFYVQPEFLG
                     NLDYVALRLATGYVKLTLFNIELLVGRDSIAWGPGYRNALLFSNNPQPFDQIKLGSAE
                     PFLLPWIGEWVGPMKLLAFLAQLENSRDHPHAKLAGMRITASPATFMELGASRAVMFS
                     GCCPTLPLSKYPQVFFNPSFGNDPNHPEERTNNLFSIDGELRFRNLDRYFFPSRDLRL
                     YGEFYWDDTCGECGPSSGLGHFLASNFLPKGSTVGGLGGVRLFRLFGQDGLDARIEYS
                     QTSVQSYNHNDFTSGYWTRGHVISDFIGTDGRDLFARVTNRLTPNLMLGLQVERAIIG
                     STVNNFAGPQEKHFGGGVDVSFRFWDRYSLFAQYLVNYVKNRGFEAGNDGLDHLVRLE
                     LTRSFR"
BASE COUNT          406 a          635 c          658 g          362 t
ORIGIN      
        1 tgccgtcacc ggcgcagccc gtgtacccgt tgcagagcag attgatccac acggtattga
       61 acccgtgtcc tcgtcgattc tcgaagaatt gctccgcctc cgcctcggtc agatcgccga
      121 tcatcgcttg cggcgactcc ccggccatga ggaacggaac attgctctgg tcgaccagat
      181 accgacctgt cggccccact ttcaccgggt agaccaccgc cgcattgacc gcgctcgccg
      241 ccagcaccaa gatggtagcg acgagcagtc ccgcaagact gcatgctctc cgcgctgggt
      301 tcacagacct cggcctcttt atttgggcgc gtcaactacg gcgaaagcgc agccaggtgt
      361 gcggggcaat cccagtgcca gaggcctggg cgaggcacaa gagcttggtt tctctagagc
      421 gaggggcttg atcctccggc cgagatccgt gaaatatttt gcacagaatg cacgcgcggc
      481 tgcatatctt gagttgccac ttctacagcg cgcgtcaccg aaaagagcgg gtcagctcca
      541 gccgaaccag gtggtcgaga ccatcgttgc ccgcctcgaa gccgcgattc ttgacgtagt
      601 tcacgaggta ctgcgcgaag agggaatacc gatcccagaa gcggaaggag acgtcgacac
      661 ccccgccaaa atgcttttct tgagggccgg cgaaattatt gaccgtgctg ccgatgatcg
      721 cgcgctcgac ctgcaatccc agcatcaagt tcggagtgag acgattggtg acacgcgcga
      781 agagatcacg accgtcggtg ccgatgaagt ccgagatgac atgaccgcgg gtccagtagc
      841 cactggtgaa atcgttgtgg ttatacgact ggacagaagt ctgcgaatac tcgatgcggg
      901 catccagccc atcctgtccg aagagccgga aaaggcgcac gcctccgagg ccgccgaccg
      961 tactgccctt cggcaggaaa ttcgaggcga ggaagtgacc aaggcccgag ctcggcccgc
     1021 actcgccgca ggtgtcgtcc cagtaaaatt ccccgtagag tcgcaggtct cggcttggaa
     1081 agaagtagcg gtcgaggtta cggaagcgca gctcgccatc gatgctgaac aggttgttgg
     1141 tccgctcctc cggatgattc gggtcgttgc cgaagctcgg attgaaaaac acctgggggt
     1201 acttgctcag cggcagggtg gggcagcagc cgctgaacat gaccgcgcgg ctcgctccga
     1261 gctccatgaa cgtggcgggt gaagcggtga tccgcatgcc ggccagcttc gcgtgcggat
     1321 gatcgcggct gttttcgagc tgggcgagga acgccagaag cttcatcggc cccacccact
     1381 caccgatcca gggcagcaga aagggctcgg cgcttcccag cttgatctgg tcgaacggct
     1441 gggggttgtt ggagaagagg agcgcgttgc gataacccgg gccccacgcg atgctgtccc
     1501 ggcccacgag cagctcgatg ttgaagagtg tgagctttac gtaaccggtc gcgagccgca
     1561 gggccacgta gtccagattg ccgaggaact ccggctgcac atagaacgtg aggaagtcac
     1621 cgatctgcag gcggctctcg aacgtcaggc cgctgtcgaa ccacttctgg aacgagccgc
     1681 cctgctcgtt gaccagagag agccggcggc tcgcgtagcc cgcgaaggcc atggcccggt
     1741 cgaccggggt gaacgagaag gtgcccgggg ccgccacggc atcgggcacc tgaaccccca
     1801 ggccggccag ctccggcttg aactcctcga tcaggcgatc gagcacaggc tccaggtcgc
     1861 gccgttcatt gagaccgccc tcgtcccggc ggatgcgctc gatggcgcgt gcgacgatcc
     1921 gcgccgcttc cacccggctg atgggcttgg tattcagaag ggttcggtcg gccagcccgg
     1981 cggtcaccag gcgctcgaga tcgtcgtaga cggcgtcctg gtggagcgag aagccgcccc
     2041 agttcttgag gggaatgttc g
//
LOCUS       DASRJQ010000461         2469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_868107, whole genome shotgun sequence.
ACCESSION   DASRJQ010000461 DASRJQ010000000
VERSION     DASRJQ010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868107"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /locus_tag="VFB89_10385"
     CDS             complement(<1..1200)
                     /locus_tag="VFB89_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27755.1"
                     /translation="MSLASRFLMGVAIAAASSCVALAAPASSEPNYQGPWWNAPPNSE
                     SGWGINLTHQGDVIFASWFTYDLGGDAWWLSMTAIKTAEGTYGGTLIETTGPAFSADP
                     FDSAKVTRTEVGNGTLTFRDADSGTLRYTLKGVSQTKFITRLVFGPLPTCTYGVGPDF
                     AAATNYQDLWWVANGAESGWGLNLAQQGDNIFASWFTYDTDGTPLWLSVTAAKTAPNV
                     YRGELIRTTGPAFDAAPFDPANVTRTVVGTTTFTFANGNSAAFAYTLNGVSQTKTITR
                     QLFVPPAGTVCGEPYRTPAPVTIVAAGDIADCGNAPAAASGAARTAALIGAEDTLVLT
                     LGDNAYKNGRPAEFANCFQPTWGAFKDRIRPSPGNHDYGTAGAEGYFGYFGAQAGPDR
                     RGYYSFDY"
     gene            complement(1330..1869)
                     /locus_tag="VFB89_10390"
     CDS             complement(1330..1869)
                     /locus_tag="VFB89_10390"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HZM27756.1"
                     /translation="MKDIENAIPAGAPNEPRILQARLQVREMAQDALASLYEVNPGTR
                     RTIERAAGYAVFSTFGLKLFFAGGTTGKGIVFNNRTQRQTFMKMLQVQGGLGFGVNQN
                     RLIFVFTNERALRNFVDQGWEFGGQANLSAMASGQGTQFSGAAAVSPGVYLYQLTETG
                     LAATITVSGTKYFKDTDLN"
BASE COUNT          413 a          788 c          810 g          458 t
ORIGIN      
        1 atagtcgaag ctgtagtagc cgcggcgatc cggaccggcc tgcgcgccga aataaccgaa
       61 gtagccttcg gcgccggcgg tcccgtagtc gtgattgccg gggctggggc gaatccggtc
      121 cttgaacgcg ccccaggttg gttggaagca gttcgcgaat tcggcgggcc tgccattctt
      181 gtaggcgttg tcgcccagcg tcagcacgag ggtgtcctcg gcaccgatga gcgcggcggt
      241 ccgggcggcc ccgctcgccg ccgcgggtgc attgccgcag tcggcgatgt ctcccgccgc
      301 gacgatcgtg accggggccg gagtgcgata cggctcgccg cagacggtgc ccgccggcgg
      361 gacgaacaac tggcgggtga tggtcttggt ctggctcacg ccgttcagcg tgtacgcaaa
      421 cgccgccgag ttgccgttgg cgaacgtgaa ggtcgtcgtg cccaccacgg tgcgcgtcac
      481 gttcgcggga tcgaacggtg ccgcgtcgaa ggcggggccg gtggtgcgga tcaactctcc
      541 gcggtagacg ttcggcgcgg tcttggccgc ggtcaccgag agccagagtg gcgtgccatc
      601 ggtgtcgtag gtgaaccacg aggcgaagat gttatcgccc tgctgcgcga ggttgaggcc
      661 ccagcccgat tcggctccgt tcgctaccca ccacaggtcc tggtaattcg tcgccgccgc
      721 gaagtccggc cccacgccat acgtgcaggt cggcagcggg ccgaatacca accgcgtgat
      781 gaacttcgtc tgtgacacac ccttgagcgt gtacctgagc gtgccagagt cggcatcgcg
      841 gaaggtcagc gtgccgttgc cgacctcggt tcgggtcacc ttggcagagt cgaagggatc
      901 cgcgctgaag gccggtcccg tggtctcgat caaggtcccg ccgtaggtcc cctccgccgt
      961 cttgatggcg gtcatggaga gccaccacgc atcgccgccg agatcgtacg tgaaccagga
     1021 ggcaaagatc acatcgcctt gatgcgtcag gttgatcccc cagccggatt cagaatttgg
     1081 gggtgcgttc caccacggac cctggtagtt gggctcggag gacgcgggcg cggcgagagc
     1141 gacacaagaa gacgccgctg ctatcgccac gcccatcaga aagcgggacg cgaggctcat
     1201 gaccgccacc gtcactcgac cgttttgcag tcgggtgacg gcgatccgtg cgagaagttc
     1261 ggtcgggtaa ttctacgcgc ctcgctgtct ctgctgcccg atgaggtcag ctcccgtgag
     1321 cgcgggacct cagttgaggt cggtatcctt gaagtacttc gtgccggaca cggtgatcgt
     1381 ggcggctagc cccgtttccg tcagctggta caggtagacg cccggggaca ccgcggcagc
     1441 gccgctgaac tgtgtcccct gtccgctcgc catcgcggaa aggttcgcct ggccgccgaa
     1501 ctcccaacct tgatcgacga agttgcgcag cgcccgctcg ttggtgaaga cgaagatcag
     1561 gcggttctga ttcactccga atccgaggcc gccctgaacc tgcagcatct tcatgaacgt
     1621 ctggcgctgg gtacgattat tgaagacgat acccttgccc gttgtgccac ccgcgaagaa
     1681 gagcttcagg ccgaaggtgc tgaaaaccgc gtagccggcg gcccgctcga tagtgcggcg
     1741 tgtgccggga ttgacttcgt agagcgaggc cagcgcatcc tgtgccattt ccctgacctg
     1801 cagccgcgcc tgcagaatcc tgggttcgtt gggcgcgccg gcgggaatgg cgttctcgat
     1861 gtccttcaac ggatcccacg aaaatatctg ggccgcgatc ggcggcgagg cgagacacgc
     1921 cgctgtggcg attattgcga agtacgtctt catcgacctt cctctccaat gggggctgat
     1981 tgcttgcgtc agaactcggt tatgcgtggc cggggactcc cgctccggca agcactgtac
     2041 ctgactacgt ggaactccgc agcgagcgct tcgcgtcgct cacgtcgcag cgtccagctc
     2101 cgaagccgca tgccctgacg ggattcgaac ctgactggcc gatccgggtt gcggcgaatg
     2161 agaaacattt tcgcccctcc cgcagcgggc gtacttgatc tcgctcaagc gcgtcgttag
     2221 tctctccccc atcgttccaa gtagcatcgc ccggtgggcg ccccatcggc accacgtgac
     2281 tgaagaggag cgcaatgcag accaccatta acttgagcgt tgccgtgcag gcttgcggtg
     2341 ccgcgattct cgccgccatc gcgattgcga gtcttcctgc cgccgcgcaa cgaccgacgt
     2401 cggcgcaagc gaacgccatc cgcgaagcat gccgcgccga tttcggcgcg aattgtgcgg
     2461 gcgttccga
//
LOCUS       DASRJQ010000462         7123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3887106, whole genome shotgun sequence.
ACCESSION   DASRJQ010000462 DASRJQ010000000
VERSION     DASRJQ010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7123
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3887106"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="VFB89_10395"
     CDS             <1..751
                     /locus_tag="VFB89_10395"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HZM27757.1"
                     /translation="SAAQRHMGSAQRDMLLGVFSLADTTVTEIMTPRIDIVAVDLSAT
                     RDDVVRTLKSSEHARLLVYDGNPDAIVGVIYAKDMLAHLNDADTSWHAFIRPGAFVPE
                     AKTLDRQLRDFQRGPSHIAIVVDEFGGTAGLVTLEDILEEIVGEIRDEYDIDEVAQIQ
                     ELGDDEWRVQGGTALTELEAALGHRFERQDVSTVGGLVLAEFGRVPRTGEMLELDGFQ
                     LIVEQIVRRRVRRVTVRRVPVPASSPGPEEE"
     gene            748..1941
                     /locus_tag="VFB89_10400"
     CDS             748..1941
                     /locus_tag="VFB89_10400"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HZM27758.1"
                     /translation="MIWLVVAVGLILAVFGAVAGGALIALSRSELARVISGQLRGGEA
                     SMGSLGQIDLDLTTASATTSLGVLLLGAAIPALLVGSGAVRSLILLLLVAVPFVLFSG
                     YFIPRWLTPSLPGGLTDRLVPVLRGWSSILRVALPARTADRPQDLRSIWREGAAVGLT
                     QDSELGLVGGVMTFAQRPVREVMTPRTELVAIAEDAALEDITQVFAQSGYSRIPVYRG
                     TLDEIIGMLHAFDLFKLQPGDPLPIRPVAVAPASRSCGDLLLDMQRERRHLAVLLDEF
                     GGTLGIATLEDLLEELVGEIFDEHDEGVQPATPGSATVFETDGTTPTSAVGEHFGVTF
                     PTGQASTIGGLVAELAGRIPNAGERFALLGLEFDVVQASPTRIERLLIRPGPASAVPL
                     ARAGT"
     gene            1938..3275
                     /gene="mgtE"
                     /locus_tag="VFB89_10405"
     CDS             1938..3275
                     /gene="mgtE"
                     /locus_tag="VFB89_10405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HZM27759.1"
                     /translation="MSALTEHHQAMIELARAGRIEAFIALAQKLEPADLADILAHLDV
                     EDRFRVVRAMPPRLSGEALVEMPEGSHPEATLAALAPAHAAEIVEELEDDDAADLLGE
                     LEPETQERILSEVEDRPDVESLLRYDEETAGGRMTTHLVTVRDGDTASQALESIRKQA
                     EEVQDFYQVFVVDGNRRLVGTLPLKDLVTSASDRPVRAFMEPADIYVQPELDQEEVAR
                     IMSRYNLPSVPVVDPAGRLLGRVTFDDLVDVVEKETTEDLLQFGGVSADEELEAGWNV
                     AVKSRLPWLYVNLLTAFMAGGVVYVFQGTVQRAVALAVWMPIIAGMGGNAGTQALAVT
                     VRRLALGLIQPTLFTKVLGKEVLVGLINGLANGLVVAVVAVLLGEGPKLGVVVFLAMT
                     GNLLVAGFAGAFIPILLERKGVDPAVASSIFVTTFTDVCGFLLLLGLAGWVLF"
     gene            3288..4922
                     /locus_tag="VFB89_10410"
     CDS             3288..4922
                     /locus_tag="VFB89_10410"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HZM27760.1"
                     /translation="MRALVVFIRLLPFVVAFLRDRRRFILFGRPARRDEAHHRRRAER
                     LTRTLAELGPTFIKLAQVFAARADILPEPYLGAIGQLTDQVPPLAAGVAEQVIASELG
                     QPAEQVFGRFDSTPLAAASLGQVHRARHGDREVVVKVLRPGVEETVRRDLDISFRVLF
                     LVNLLFPNHHTRAITTIVTEFSKRISSELDFRDEARNAEILRRNFAAEPRVVVPEVIQ
                     PLTRRRVLVLEYVEGTRIDRLQQRLDSGELSLDLLIGTIVEVYIKMMLEDGFFHADPH
                     AGNLLVDKLGRIVLLDFGMVVQVERETRARMVRTVTAAVRQDVDGVINGFYELGILDP
                     DVDRGTVRDAAQQLMGVALRDNVSPRQMQRIVEDVLRTFYDWPLMLPSDLAYFGRAAV
                     LVEGIGLRYDPNFNALAAARPVVAQSAPRLLHGLLGGDGRARIADWSLEAAATVRTVR
                     DLLRRVERDELRVRWHPRDTVELQRFLAQQVRRTLLALFALGLGVMAAVVYAATRRLE
                     VLAVGLVVSIGMFVLVFVLPSHLFQNPLRFRRRWGR"
     gene            complement(4944..5306)
                     /locus_tag="VFB89_10415"
     CDS             complement(4944..5306)
                     /locus_tag="VFB89_10415"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HZM27761.1"
                     /translation="MLLRRAIVAGSFLALLTSGALWAQAKPAAPPSQPAAPAPAGANK
                     ADTGKMAGHNVASTDLLDINTASKDQLEALPGIGSAYADKIIKNRPYKRKDELVSKKV
                     LPNSVYTKIKDQVIAKQK"
     gene            complement(5358..6794)
                     /locus_tag="VFB89_10420"
     CDS             complement(5358..6794)
                     /locus_tag="VFB89_10420"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZM27762.1"
                     /translation="MTGTEGEASVLLVDDDEAACRLLAEVLERDGYRVASALSVDEAL
                     AKLDREGPFDAVLTDLRMPERSGLDLLKTVRDREPDALVLVLTAFGDAAAAGEAIRAG
                     AYDFVSKPYDIAALRETLARALGRRRLASASRERRGAAQRPSGGSRGGSLVGHSLAII
                     EVMKTVARVAPTQATVLLLGETGTGKELVARTIHSFSERNGRRFVAVNCSALAEGLLE
                     SELFGHIRGAFTGAAASRPGLFREADHGTLFLDEIGDISPALQARLLRALQEHEIVPV
                     GSEAAVKVDVRVIAATHRDIASLVRTGKFREDLYYRLNVVMLQLPPLRERKQDIPLLL
                     DHFLRELTERHGRGPVALDSEAQHRLLAYDWPGNVRELQNVLERALVLAEQDVIGSEH
                     LPPEVKPNGGPELSPEPAPAERGIGSPPSASPLLSLEEVERRHVMRVLEATGGSREEA
                     SRILGISRRTLTRMVQRWGLPPRRHIGA"
     gene            complement(6791..>7123)
                     /locus_tag="VFB89_10425"
     CDS             complement(6791..>7123)
                     /locus_tag="VFB89_10425"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZM27763.1"
                     /translation="CDPAQVEQILLNLLKNAIEALEPGGLVIVSTGIRNGQACLEVED
                     DGPGLDLEAQANLFRPFTTTKGAGGSGLGLAVSRRLARGLGGDLEHVPSSAGTRWRLT
                     LPSAGVAA"
BASE COUNT         1120 a         2284 c         2421 g         1298 t
ORIGIN      
        1 cagtgccgcg cagcgacaca tggggagcgc gcaacgcgac atgctgctcg gtgtcttctc
       61 gttggccgac accacggtca ccgagatcat gacgccgaga atcgacatcg tcgcggtgga
      121 cctgagcgcc actcgcgatg acgtcgtgcg gaccctcaag agctccgagc atgcccggct
      181 cctggtatac gacggcaacc cggacgccat cgtgggcgtg atctacgcga aagacatgct
      241 ggcgcacctg aacgacgcgg acacctcctg gcacgcgttc atccgccccg gcgccttcgt
      301 ccccgaggcg aagacgctcg atcggcaact gcgcgacttc cagcgcgggc cgagtcacat
      361 cgcgatcgtg gtcgatgagt tcggcgggac ggcaggcctg gtgacgctgg aagacatcct
      421 ggaggagatc gtcggggaga ttcgcgacga gtacgacatc gatgaagtcg cccaaatcca
      481 ggagctggga gacgacgagt ggcgggtgca aggcggcacg gcgcttaccg agctcgaggc
      541 cgccctcggc catcgcttcg agcgccagga tgtgagcaca gtgggtggcc tggtactggc
      601 ggagttcgga cgcgtgccgc ggaccggcga gatgctcgag ctggacggct tccagctcat
      661 cgtcgagcag attgtgcgcc gccgggtgcg ccgggtgacc gtgcgccggg taccggtgcc
      721 ggccagctcg ccggggccgg aggaagaatg atctggctgg tcgtggcggt cggtctgatt
      781 ctagccgtgt tcggcgcggt ggccggtgga gccctcattg ctttgagccg gagcgagctg
      841 gcgcgcgtca tctcgggcca gcttcgtggt ggcgaggcat cgatggggtc gctcggacag
      901 atcgacctgg atttgacgac ggcttcggcc acgacatccc tcggcgtgct tctgctcggc
      961 gccgcgattc cggctctgct ggtggggtcg ggggccgtgc gctcgctcat cctgttgctg
     1021 ctggtggccg ttccgttcgt tctcttcagc ggctacttca tccctcgatg gttgacacca
     1081 tcgctcccgg gcggactgac cgaccgtctg gtgccggtcc tccggggctg gtcgtcgatt
     1141 ctgcgtgtgg cgctccccgc ccgtactgcc gatcggcccc aggatctccg atccatctgg
     1201 cgggaaggtg ccgccgtcgg cctgacccag gacagcgagc tgggattggt gggcggtgtg
     1261 atgacctttg cccagcggcc ggtgcgcgag gtgatgacgc cgaggaccga gctggtagcg
     1321 atcgccgaag atgcggcact cgaagacatt actcaggtct ttgcgcaaag tggctacagt
     1381 cgcatccccg tttatcgcgg cacgctcgat gagatcatcg ggatgctcca cgcgttcgat
     1441 cttttcaagc tgcagcccgg cgatccgctg ccgatccggc ctgtggctgt ggcacccgcc
     1501 agccgaagct gcggtgatct cctgctcgac atgcagcggg aacggcgcca cctcgccgtg
     1561 ctgctcgacg agttcggcgg cacgcttggc atcgcgactc tggaggatct gctggaagag
     1621 ttggtcggcg aaatcttcga cgagcacgac gaaggagtcc agccagctac ccccggctcg
     1681 gcgaccgtct ttgagaccga cggaacgacc ccaacgtcag ccgtcggtga gcatttcggg
     1741 gtgactttcc ccaccggcca ggcgagcacg atcggcggcc tggtcgccga gctggcgggc
     1801 cgcattccta acgcagggga gcgcttcgcg ctgctcgggc tcgaattcga cgtcgtgcag
     1861 gcctcaccca cccgtatcga acggcttctg atccgacccg gtccggcctc cgctgtgccg
     1921 ctcgcccggg ccggaacgtg agtgcgctga ccgaacatca ccaggcgatg atcgagctgg
     1981 cccgcgccgg acgcatcgaa gcgttcattg cgctggccca gaagctcgag cctgcagacc
     2041 tcgccgacat cctcgcacat ctcgacgtcg aagaccgctt ccgggtggtc cgagcgatgc
     2101 cgccgcgcct ctcgggcgag gcgctggtcg agatgcccga aggcagccat ccggaggcga
     2161 cgctggccgc gctggcgccg gcccacgcag ccgagatcgt cgaagagctg gaggatgacg
     2221 acgcggccga cctgctgggc gagctcgagc ccgagacaca ggagcgcatc ctttcggagg
     2281 tcgaggatcg gcctgacgtc gaaagcctgc tgcggtacga cgaggagact gcgggtggtc
     2341 ggatgaccac gcacctcgtc acggtgcgtg acggggacac cgcaagtcag gcgctcgagt
     2401 ccatccgaaa gcaggccgag gaggtacagg acttctatca ggttttcgtg gtggacggca
     2461 accggcgcct ggtcggcact ttgccgctca aggacctggt gacgagcgca tctgatcggc
     2521 cggtgcgcgc gttcatggag ccagccgaca tctacgtgca gcccgagctg gaccaggaag
     2581 aggtggcgcg tatcatgtcg cgctacaacc tgcccagcgt tcccgtggtg gatccggccg
     2641 gccggctgct gggacgggtc acgttcgacg acctggtgga cgtggtcgag aaagagacca
     2701 cggaggacct gctgcaattc ggcggcgtct cggccgacga agagctggag gcaggctgga
     2761 atgtcgcggt gaagagccgc ctcccctggc tctacgtcaa cctcctcacc gcgttcatgg
     2821 caggcggtgt cgtgtatgtc ttccagggga ctgtgcaacg ggctgtggcg ctggcggtat
     2881 ggatgccgat catcgccgga atgggcggta atgccggaac gcaggcgctc gcggtaacgg
     2941 tgcggcggct ggccctgggg ctcattcagc ccaccctgtt caccaaagtg ctcgggaagg
     3001 aagtgctggt gggtctaatc aacgggctcg cgaacggtct ggtcgtcgcg gtcgtggccg
     3061 tcttgctggg agaaggtccc aagctcggcg tggtcgtatt tcttgccatg acgggcaatc
     3121 tgctggttgc gggattcgcc ggggccttca ttccgattct gctcgaacgc aagggggtgg
     3181 acccggccgt ggcctcgtcc atcttcgtga cgacatttac cgacgtttgt ggatttctgc
     3241 tgctgctcgg tctggcgggg tgggtgctgt tctagcatcc ggtttcaatg cgtgccctcg
     3301 tagtctttat ccgtctcctg ccattcgtcg tcgcctttct gcgcgaccgc cggcgattca
     3361 ttctcttcgg gcggcccgcc cggcgagacg aggcgcatca tcggcgtcga gccgagcggc
     3421 tcacccggac gctcgccgag ctggggccca cgttcataaa gctcgcgcag gtcttcgcag
     3481 ctcgggccga catccttccg gagccgtatc tgggcgcgat cggccagctc accgatcagg
     3541 tcccgccgct tgccgcgggc gttgccgagc aggtgatcgc gagcgagctg gggcagcccg
     3601 cagagcaggt gttcgggcgg ttcgattcga cgccgctggc ggcagcttca ctgggccagg
     3661 tccaccgcgc ccgtcatggg gaccgcgagg tggtcgtgaa ggtgctcagg cccggggtgg
     3721 aggagaccgt tcgtcgcgat ctcgacatct cgtttcgagt cctgtttctg gtcaacttgc
     3781 tgtttcccaa ccaccatact cgcgcgatca cgaccatcgt caccgaattc tccaagcgga
     3841 tttcgagcga gctcgatttt cgggatgaag cgcgcaacgc ggaaattctg cggcgtaact
     3901 tcgcggcgga gcctcgcgtc gtggtcccgg aggtgatcca accgctcacc cggcgtcgcg
     3961 tgctggtgtt ggagtatgtc gaaggcaccc ggatcgatcg cctgcagcag cggctcgact
     4021 ccggcgagtt gtcgcttgac ctgctgatcg ggactatcgt cgaggtctac atcaagatga
     4081 tgctggagga cggcttcttt cacgccgatc cccatgccgg gaatctgctg gtcgacaaat
     4141 tgggccgcat cgttctcctc gatttcggca tggtggtgca ggtcgagcgc gagacccgtg
     4201 cgcggatggt gcgaacggtg acggcggcgg tgcggcagga tgttgacggc gtcatcaatg
     4261 gcttctacga gctgggcatt ctcgatcccg acgtggaccg cgggaccgta agggatgcag
     4321 cccagcagct catgggggtg gccctacgcg ataacgtgtc gccccgccag atgcagcgta
     4381 tcgtcgagga cgtgctccgc acattctatg actggccgtt gatgctgccg tcagacctgg
     4441 cgtacttcgg tcgggcggca gtgctggtgg aaggcattgg cctccggtac gatccgaatt
     4501 tcaatgcact cgcggctgcc cggcccgtgg tcgcgcagtc ggcaccgcgg ctgctgcatg
     4561 gcttgctggg aggagatggt cgcgcccgca tcgccgattg gagtctggaa gccgcggcga
     4621 cggttcgcac cgtcagagac ctgcttcggc gagtagagcg ggacgagctt cgggtccgct
     4681 ggcacccgcg tgacacggtc gagctgcagc gctttctcgc ccaacaggtc cgccggaccc
     4741 tgcttgcgct cttcgcgctc gggctcggcg tgatggcggc ggtcgtatat gcagcaaccc
     4801 gacggctgga ggtgctggcc gtcgggctgg tcgtgtcaat cgggatgttc gtcctggtct
     4861 tcgtactgcc cagtcacctg tttcagaatc cgttgcgatt cagacggcgc tggggtaggt
     4921 aggccttcaa ccgctggcca atcctacttc tgctttgcaa tcacctgatc cttgatcttc
     4981 gtgtagacgc tgttcggcag caccttcttc gataccagct cgtccttccg cttgtacggg
     5041 cgattcttga tgatcttgtc ggcataggcg ctgccgattc ccggcagggc ctcgagctga
     5101 tccttgctgg ctgtattgat atcgagcaga tcggtgctgg cgacattatg gccggccatc
     5161 ttgcccgtat cggccttgtt ggcgccggcc ggagctgggg ccgcgggctg tgaaggcggg
     5221 gccgcaggct tggcttgcgc ccacaacgcg ccactcgtga ggagggcgag gaaagatccc
     5281 gcgacaatag cgcgacgaag cagcatggaa acctccggat gtggttcggc ggagggcaga
     5341 aagcgcaggg tccgtgccta agcgccgatg tgacgccggg gtggcagccc ccaccgctgg
     5401 accatccggg tgagcgtgcg gcgggagatg cccagtatgc gcgacgcttc ctctcggctt
     5461 ccaccggtcg cttcgaggac ccgcatgacg tgccggcgct cgacctcctc cagcgacagc
     5521 aatggactgg ccgacggtgg cgacccgatc ccccgctcgg ccggggccgg ctcgggcgac
     5581 agctccgggc ctccgttggg cttcacttca ggcggaagat gctccgaacc gatcacgtcc
     5641 tgctcggcca gaaccagcgc acgctcgagg acgttctgca gctcgcgaac gttgcccggc
     5701 caatcgtagg ccagcaaccg atgctgcgcc tcggaatcca aagccaccgg gccgcgtcca
     5761 tgccgctcgg tcagctcgcg caggaaatga tcgagcagaa gcgggatgtc ctgcttccgc
     5821 tcccgaaggg gcggcagctg aagcatcacc acgttcagcc ggtagtagag gtcttcgcga
     5881 aacttgccgg tgcgcacgag actggcgatg tcgcggtgcg tggccgcaat gactcgcacg
     5941 tcgactttga ctgccgcctc cgagccgacc ggaacgatct cgtgctcctg gagcgcgcgc
     6001 agcaggcggg cttgcagggc aggggaaatg tcgccgatct cgtcgaggaa cagcgtgccg
     6061 tgatccgcct cgcggaacaa acccggccgg gacgctgccg cgccggtgaa ggccccgcgg
     6121 atgtgcccga acagctcgct ctccagcagg ccttcggcta gggccgagca gttcacggcc
     6181 acgaatcgtc ggccatttcg ctcggaaaag gagtggatgg ttcgggctac gagctccttg
     6241 cccgtcccgg tttctcccag gaggagcacg gtggcctggg tgggagcgac ccgggcaacg
     6301 gttttcatga cctcgatgat cgcgaggctg tggcccacca gcgatccacc acggctgccg
     6361 ccggagggtc gctgggcagc gccgcggcgc tcgcggctcg cgctcgccag ccgacggcga
     6421 cccaatgcgc gggccagggt ctcccgcagc gcggcgatat cgtacggctt gctcacgaag
     6481 tcgtaggcgc ccgcccggat ggcttcgcca gcagcggccg cgtcgccgaa cgccgtaagc
     6541 accagcacca gcgcatcggg ctcccgatcc ctaacggtct tgagcagatc gagcccactt
     6601 cgttccggca tccgcaaatc cgtgagcacc gcgtcgaacg ggccttctcg gtctagcttg
     6661 gccagggctt cgtccaccga cagcgcggag gcgacgcgat agccgtcccg ctcgagcacc
     6721 tccgctagca accggcaggc ggcttcgtcg tcgtcgacca gcagaacgct ggcttcgccc
     6781 tcggtgccgg tcatgcagct actcccgcgc tgggcaaggt gaggcgccag cgggttcccg
     6841 cgctgctggg aacgtgctcc aggtcgccgc cgaggccacg ggcgagccgc cggctgacgg
     6901 ccaatccgag cccgctgccc cccgctcctt tggtagtggt gaacggccgg aagagattgg
     6961 cctgcgcttc aaggtcgaga cccggaccat catcttcgac ctcgagacaa gcctgcccat
     7021 tccggatacc ggtgctcacg atcaccaggc cacccggctc cagcgcctcg atcgcattct
     7081 tgagcaggtt caagaggatc tgttcgacct gagccgggtc gca
//
LOCUS       DASRJQ010000463         2467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1473615, whole genome shotgun sequence.
ACCESSION   DASRJQ010000463 DASRJQ010000000
VERSION     DASRJQ010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473615"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VFB89_10430"
     CDS             <1..191
                     /locus_tag="VFB89_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27764.1"
                     /translation="IPTTLHPQVMDRFHAMFDIPKDMTFHFCIPLGYSAVKYGPSRRK
                     PIAETTSWNHWAGPVPWS"
     gene            188..1096
                     /locus_tag="VFB89_10435"
     CDS             188..1096
                     /locus_tag="VFB89_10435"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZM27765.1"
                     /translation="MTRKPAVSLAAVPGRRRATIEMAQRLEREGFSGIYCPSLGDGVA
                     LCEALALMTREIPFGTSIANLYSRHPSDYAHTAAFLHEMSGGRFRFGIGVSHSPAHER
                     LGVTPGKPLAEVRRFMKDLESWTKQTGELPPIVLATLRKGMVALAGEIAQGAVWANAA
                     RSHMTTSLGHLPASARQSPRFFIGNMVPTCIDDDRAAAAAVNRRTLTSYVKLPNYQNY
                     WIEAGFEDEMQAIRRAIAAGEEGKIPELMSDRWLSQVTLYGNATEVRDGVEKWYETGI
                     NTLIIVPSSARGNQMVALQQLIDLYR"
     gene            1132..1590
                     /locus_tag="VFB89_10440"
     CDS             1132..1590
                     /locus_tag="VFB89_10440"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HZM27766.1"
                     /translation="MLKLETPKDVLAHIGTELGPSEWLTVTQEMIDKFAEATGDHQWI
                     HVDVERAKREMPGGKTIAHGYLTLSLLPRLAPTLMSVAKRRRGINYGSNKIRFISPVP
                     AGSRIRLRQKLLKAEPVEDNGIRITSQMTMEVEGGERPAMVAETVSVVYA"
     gene            1975..>2467
                     /locus_tag="VFB89_10445"
     CDS             1975..>2467
                     /locus_tag="VFB89_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27767.1"
                     /translation="MKMLAGAVGVIGALVLAVTPAMAGIGGSNVPTLPLTAQVGEIFE
                     ANTLIINGSTDPNDTENVTLTTLFVTPSCADSTSNVCISPNLDPGVFKVLSATGDGVL
                     NPCAGVTFDVGMPDPTTGEVALVPQTTVTLGPATGPLIDRTCRVNILLRVFKVPTNPA
                     PGPP"
BASE COUNT          507 a          785 c          740 g          435 t
ORIGIN      
        1 ccattccgac gacgctccat ccgcaggtga tggaccgctt tcacgcgatg ttcgacattc
       61 cgaaggacat gacgtttcac ttctgcattc cgctcggcta ttcggccgtc aagtacggcc
      121 cgagccggcg caagcccatc gccgagacca cgtcgtggaa ccactgggcg ggcccggtgc
      181 cgtggtcatg acacgcaagc cggccgtgag cctcgctgcc gtgccgggcc ggcggcgagc
      241 caccatcgag atggcgcagc gtctcgagcg tgagggattc agcggaatct attgtccgag
      301 cctcggcgac ggcgtggctc tgtgcgaagc gctggcgctg atgacgcgcg agatcccgtt
      361 cggtaccagc atcgccaatc tctactcgcg gcacccatcc gactacgcgc acacggccgc
      421 gttcctccac gagatgtcgg gcgggcgctt ccgcttcggg atcggcgtga gtcacagccc
      481 tgcgcacgaa cggctcgggg tgacgccggg caagccgctc gccgaggtgc ggcggttcat
      541 gaaggacctc gagagctgga cgaagcagac cggtgagctt cccccgatcg tgctcgccac
      601 cctcaggaag ggcatggtcg ccctcgccgg cgagatcgct cagggcgccg tatgggccaa
      661 tgcggcccgt tcgcacatga ccacgtcgct cggccacctg cccgcgagcg cgaggcagag
      721 tccgcgtttc ttcatcggga acatggtgcc gacgtgcatc gacgatgacc gggctgccgc
      781 cgccgcggtc aaccggcgca cgctgacgag ctacgtgaag cttccgaact atcagaacta
      841 ctggatcgag gccggattcg aagacgaaat gcaggccatc cgccgagcga ttgccgcagg
      901 cgaggaaggg aagatcccgg agctcatgtc cgaccgctgg ttgtcccagg tcacgctcta
      961 cggcaatgcc accgaggtcc gcgatggcgt cgagaagtgg tacgagacgg gcatcaacac
     1021 gctgatcatc gtgccgtcct ccgcccgcgg caaccagatg gtggcgctcc agcagctcat
     1081 cgatctctac cgctgacccc ggcgcagcgg acatcggagg gaggccgcgc gatgctgaaa
     1141 ctcgagacgc ccaaggatgt actcgcccat atcggcaccg agctgggccc gtcggaatgg
     1201 ctcaccgtca cgcaggagat gatcgacaag ttcgccgagg cgaccggcga tcaccagtgg
     1261 atccacgtcg acgtggagcg cgccaagcga gaaatgccgg gcgggaagac catcgcgcac
     1321 ggttacctga cgctttcact cctgccacgg ctggccccca cgctgatgag cgtcgcgaag
     1381 cggcggcgtg ggatcaacta cgggtccaac aagatccgct tcatcagtcc cgtgccggct
     1441 ggctccagga ttcggctccg tcagaagctc ctcaaggccg agcccgtcga ggacaacggc
     1501 atccgcatca cgagtcagat gaccatggaa gtggaaggag gcgagcggcc cgccatggtt
     1561 gccgaaaccg tttccgtggt ctacgcgtag gactaataag ccgttacgag gacttacggc
     1621 tctcgggtca gcaagaaaac acaagtctcc aaacaaaagg ctactgtgtc caagtttcgg
     1681 taaacgctcc attagacagt gcaatctcgc tgtcttgcgc aagaaagaac aaattaatca
     1741 caataaacta ctagcatcag cctcctttta ttgcataatt gcgggaccac cgaaggaagc
     1801 ctacgaaaca ggtagcaaat cgttgtcact tccacggttg acatgtcagg tttcagacac
     1861 ttgcggatga gcacggggtt ggcctcggtc ttgcctttcc ctatgtggcg aacaaacccc
     1921 caattcctac aggaggagta gctgatgagt tttcgacgaa caaggatgtg gtctatgaaa
     1981 atgctggcgg gcgcagttgg agtgatagga gcattggtcc ttgcggtgac gccagcgatg
     2041 gcgggaatcg ggggaagcaa tgttcctaca ctgcccctca ctgcccaagt cggagagatc
     2101 ttcgaagcta ataccctcat catcaacgga tcgaccgacc ctaacgacac cgagaacgtt
     2161 accctgacga ccctcttcgt cacgccgtcc tgcgcggatt ccacgagcaa tgtatgcatt
     2221 tcccccaact tggatcctgg cgtcttcaaa gtgctttcgg cgaccggcga tggggtcttg
     2281 aacccctgcg ccggcgtcac cttcgacgtc ggaatgccgg atcccaccac cggagaggtg
     2341 gcgctggttc cgcagacgac cgtcacgctc ggacccgcca ccgggccctt gatcgatagg
     2401 acctgccggg tcaatatcct actccgagtg ttcaaagtcc cgacgaatcc ggcccccggc
     2461 cctccta
//
LOCUS       DASRJQ010000464         2075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2664669, whole genome shotgun sequence.
ACCESSION   DASRJQ010000464 DASRJQ010000000
VERSION     DASRJQ010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2664669"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VFB89_10450"
     CDS             complement(<1..308)
                     /locus_tag="VFB89_10450"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZM27768.1"
                     /translation="MRDGDGGALTKQAINPPAVFRSLEHGFSQAVVASGTRTLYVSGQ
                     TAWDSGRQLVGGADLEMQARQAFTNLRTVVEAAGGTLADVVAVRIYVVDYRPEKAASV
                     G"
     gene            complement(320..1252)
                     /locus_tag="VFB89_10455"
     CDS             complement(320..1252)
                     /locus_tag="VFB89_10455"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZM27769.1"
                     /translation="MHRTNFLARMAMAWLVLLGLSVTAAAQGRVEKNVVYGMYSGSAL
                     LLDVHYPAQANGFGIVFIAGSGWNAPLGYAAAPLKESPQVEIYVPSLTQAGYTVFAVT
                     HRATPTFRFPAPVEDAQRAVRFIRHNAAKYGIDAARIGGSGGSSGAHLISMLATMDGT
                     DDPADADPVNRESAKLQCVVARAAPIDLLRMNPSGGAAALALLLGANVTEATPKTSAE
                     YKAALAASPINYVSKDDPPMLLVHGDADRTVPFNQSEVMEAALRKAGVPVQLIRIEGG
                     DHGPTFPGARNPPDYKAAMVKWFDEHLRRASTDR"
     gene            complement(1264..>2075)
                     /locus_tag="VFB89_10460"
     CDS             complement(1264..>2075)
                     /locus_tag="VFB89_10460"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZM27770.1"
                     /translation="THTSGLGYGFTSPTLRDFKPREGETYPAGPLLFEPGTQWIYGTS
                     VDWLGRLVEKLSGKSLEEYFRERIFTPLKMPDTFYNVPETKQARLVTVHRRPDGRADA
                     PLVEQPSQPPRPATSFNGGGGLSSTANDYMRFERMILNGGSLDGARILSSDSVALMGK
                     DQIGDVGVAALKTAQPDLSMDFTFIDDGKDKWGLGFLRTTRQVPGKRSAGSLSWGGVY
                     NTYFWIDPARGIAGVILMQFLPFADTKALAIYDAFERGVYQLADAPLKSNR"
BASE COUNT          372 a          676 c          649 g          378 t
ORIGIN      
        1 cccactgacg cggctttctc gggtcggtag tccaccacgt agatccgcac cgcaaccaca
       61 tcagcgaggg taccgccggc ggcctcgacg accgtcctca gattggtaaa cgcctgtctc
      121 gcctgcattt cgaggtcggc tccgccgacg agctgcctgc cggaatccca cgccgtctga
      181 ccggagacgt aaagtgtcct cgttccgctg gcgacgacgg cctgcgagaa gccatgttcg
      241 aggctccgga agaccgcggg tggattgatc gcctgcttgg tcaacgcccc gccgtctcca
      301 tccctcaagg tccgtccaat tacctgtcag tcgaagccct gcgaaggtgc tcgtcgaacc
      361 acttcaccat ggctgccttg tagtccggcg gattcctggc tccgggaaac gtcgggccat
      421 ggtcaccgcc ctcgatgcgg atcaactgca ccggcacgcc cgccttccgt agcgcagcct
      481 ccatcacttc ggattggttg aacggcacgg ttcgatcggc atcgccatgg acgagcagca
      541 tgggtggatc gtccttcgac acgtagttga tgggcgacgc ggccaaggcc gctttgtact
      601 cggcggaagt cttcggcgtt gcctcggtca cgttggcgcc aagtaacagc gccagcgcag
      661 cggcaccgcc gctggggttc atccgaagga gatcgatcgg agcggctcgc gcgacaacgc
      721 actggagctt ggcgctctcc cggttcaccg gatcggcatc ggcggggtcg tctgtgccgt
      781 ccatggtggc cagcatgctt atgaggtggg caccagagga gccgccggag ccaccgatac
      841 gggcggcatc gattccgtac ttcgcggcat tgtgtctgat aaaccggacg gcccgctgcg
      901 cgtcctccac aggtgcggga aaccggaacg tcggtgttgc gcgatgcgtc accgcaaaga
      961 ccgtataccc agcttgcgtg agcgagggaa cgtagatctc gacttggggt gattccttca
     1021 gcggcgcggc tgcatagccc agcggggcat tccagccgct gccggcgatg aagacgatgc
     1081 caaagccatt cgcctgcgcc ggatagtgaa catccaacag cagcgccgaa cctgagtaca
     1141 tcccatacac gacattcttt tcgacgcggc cctgagcggc agcggtgacg ctgagtccaa
     1201 gcaagaccag ccatgccatg gccatgcgtg caaggaaatt cgtgcgatgc atgctacctc
     1261 ctcctaacgg ttcgacttga gcggcgcgtc ggccagctga tacacaccgc gctcgaaggc
     1321 gtcgtagatc gcgagcgcct tggtgtcggc aaacggaagg aactgcatca ggatcacacc
     1381 ggcgataccg cgcgccgggt cgatccagaa gtacgtgttg tagacaccgc cccagctgag
     1441 gctgcctgcc gaccgctttc ccggcacctg ccgggtcgtg cggaggaatc ccagtcccca
     1501 cttgtccttc ccgtcgtcga tgaacgtgaa gtccatgctc aagtccggct gtgcggtctt
     1561 cagagcggca acgcccacgt cgccgatctg gtctttgccc atgagggcca ccgagtcgct
     1621 ggaaagaatc cgcgcgccat cgaggctgcc gccgttcagg atcatccgct cgaagcgcat
     1681 gtagtcgttc gcggtcgacg acagcccgcc gccgccgttg aaggacgtgg ccgggcgcgg
     1741 cggctgactg ggctgctcga cgagcggcgc atcagcgcga ccatcgggcc gccggtggac
     1801 cgtcacgagc cgcgcctgct tggtctcggg gacgttgtag aacgtgtcgg gcatcttcag
     1861 cggcgtgaag atacgctcgc ggaagtactc ctcgaggctt tttccggaga gcttctcgac
     1921 taaccgcccg agccaatcga cgctcgtccc atagatccac tgcgtccctg gctcgaagag
     1981 caacgggccc gccggataag tttcgccctc gcgcggcttg aagtcgcgga gggtcggact
     2041 cgtgaatccg tatccaagcc cggaagtatg tgtaa
//
LOCUS       DASRJQ010000465         4141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1285516, whole genome shotgun sequence.
ACCESSION   DASRJQ010000465 DASRJQ010000000
VERSION     DASRJQ010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285516"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VFB89_10465"
     CDS             <1..264
                     /locus_tag="VFB89_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27771.1"
                     /translation="QATGEAGTRARQIADAAAAQQSAFQQLTGRIEQVAAVSARTQAQ
                     TETLAGQATEAARGQAELEQAIRELEGVATHLQRIARHFAVGA"
     gene            261..680
                     /gene="folK"
                     /locus_tag="VFB89_10470"
     CDS             261..680
                     /gene="folK"
                     /locus_tag="VFB89_10470"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HZM27772.1"
                     /translation="MSEHRGEQVFVALGSNVGERAEHLAAARDAIGRLPETRLVAASS
                     IEETAPLAGMRQPPYLNQMVLVETDLDPHELLRALQRIERERGRVRAERWGPRTLDLD
                     IVKFGNRRIDAPDLVVPHPELPNRDFWQRELAELSRS"
     gene            677..1486
                     /gene="panB"
                     /locus_tag="VFB89_10475"
     CDS             677..1486
                     /gene="panB"
                     /locus_tag="VFB89_10475"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HZM27773.1"
                     /translation="MSSESPPRTVLDLTAMKASGRKIVMLTCYDAIFARLIAPEVDVL
                     LVGDSVNQVVAGHTTTLSATLEQLIYHTRAVRRGAPNSLVLVDLPFLSYQVSVEDAIR
                     NAGRVLQKTGAQGVKLEGGRHVVATVRALVERGIPVMGHLGLTPQSVHALGGHRIQGR
                     ETGAPERMRDDARELEDAGAFGLVLELVPAGLARRISETLRIPTIGIGAGAGCDGQVL
                     VLHDMLGLNETFSPRFLKRFASLAGTVRDAARAYAKEVRAGTYPDDQHSFE"
     gene            1483..2334
                     /gene="panC"
                     /locus_tag="VFB89_10480"
     CDS             1483..2334
                     /gene="panC"
                     /locus_tag="VFB89_10480"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HZM27774.1"
                     /translation="MTAMLEISEIGALRHWRDDQRHAGHRVALVPTMGSLHEGHLALV
                     DEARRRADAVVLSVFVNPLQFGPEEDFERYPRNLERDRGLAAGRGVDLLFAPETEALY
                     PQGSEIRIVPGETAERWEGSIRPGHFAGVLTVVAKLLHLVEPDVACFGQKDIQQVTLV
                     RRMIRDLDWSVEIAVVPTVREADGLAMSSRNIYLSPADRAKALSLSRGLRAARDAWQG
                     GERSASVLKRIVQSSLRAATGVRAEYIAVVEPEKLRPVAVAPAGTILAVAARVGNTRL
                     IDNVILT"
     gene            2343..2714
                     /gene="panD"
                     /locus_tag="VFB89_10485"
     CDS             2343..2714
                     /gene="panD"
                     /locus_tag="VFB89_10485"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HZM27775.1"
                     /translation="MQRHLMKSKIHRATITAADLHYEGSLTVDEDLLDAADLVTHEEV
                     QVVNVNNGSRFTTYVIPGPRGSGVMQLNGAAARLGHPGDVVILISYGVFTDAEAKRHV
                     PRVVFVDERNRLVRTPLRAES"
     gene            2825..3322
                     /locus_tag="VFB89_10490"
     CDS             2825..3322
                     /locus_tag="VFB89_10490"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZM27776.1"
                     /translation="MQRVAELAASWAEAMGVPDNERHRWLRAVWLHDALRDAPPEELD
                     RWASSAPVPASLKHGPAAAARAKAEGEVDRGVLDAVRYHSVGLAEWDMVGRVLYAADY
                     LEPGRSFDRERRAELARRFPADSRGVLREIAGERLAHIVASGWPLPEPTVRFWNSLVT
                     PSSSR"
     gene            3286..3723
                     /locus_tag="VFB89_10495"
     CDS             3286..3723
                     /locus_tag="VFB89_10495"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HZM27777.1"
                     /translation="MELSRHPVVIALIVLGVLGGTVAAVALRPPLPAPTPIKHAYAVP
                     AREHRILVEVLNTTTRPGLARSATRVLRRQSLDVVFFGNADGSAKVDSTQVIARRGNR
                     GAAQTVAKVLGQGVVKLQTDTLRRVDVTVLLGSDYRPPVDGHP"
     gene            complement(3791..>4141)
                     /locus_tag="VFB89_10500"
     CDS             complement(3791..>4141)
                     /locus_tag="VFB89_10500"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZM27778.1"
                     /translation="TGVPIPGGSDAPVELGEPMIEFYAAVARKDTSGFRGPDWHPEQA
                     LTRGEALKMLTLWPAFAAFQEQQRGSIEVGKWADLTVLSADIMQIPEREILKTRCVMT
                     VVGGEVAFEEKRER"
BASE COUNT          699 a         1285 c         1409 g          748 t
ORIGIN      
        1 caggccaccg gcgaggcagg aacgcgagcg cggcagattg ccgacgcggc cgcggcgcag
       61 cagagtgcgt ttcaacagct gaccggccgc atcgagcagg tcgcggcggt ctccgcgcgc
      121 acccaggcac agacggaaac gctggcgggt caggcaaccg aggccgcgcg gggacaggcc
      181 gagctggagc aggccattcg tgagctggaa ggtgtggcga cccatctcca gcggatcgcg
      241 cgccattttg cggtgggcgc gtgagtgagc accgcggaga gcaggtgttc gtggcgctgg
      301 ggagcaatgt gggcgagcga gccgagcatc tcgcggctgc acgtgacgcc atcgggcgtc
      361 tacccgaaac cagactggtc gcggcgtcct ctattgaaga gaccgctccg ctggcgggga
      421 tgcggcagcc accctatctc aatcagatgg tcctcgtcga aacggatctc gatccccacg
      481 aattgctgcg ggctcttcag cgaatcgagc gcgagcgagg acgggttcgc gccgaacgct
      541 ggggaccgcg gacgctcgat ctcgatatcg tcaagttcgg caaccgccgg atcgacgccc
      601 ccgaccttgt tgtccctcat cctgagcttc ccaatcgcga cttctggcaa cgcgagctgg
      661 cggaattgag tcgttcatga gctccgagtc accaccccga accgtgctgg atctgacggc
      721 catgaaggcg tccggccgca agatcgtcat gctgacatgc tacgacgcga tcttcgcgcg
      781 tctgatcgca ccggaggtag acgtgctcct ggtgggcgat tccgtgaatc aggtcgtcgc
      841 cggccacacc accactttga gcgcgacact cgagcagctc atctaccata cgcgggccgt
      901 tcggcggggc gcacccaaca gcctggtgct ggtggatctt ccatttctct cgtaccaggt
      961 gtcggtggaa gatgccatcc ggaacgcggg gcgggtgctg caaaagaccg gggcgcaggg
     1021 cgtcaagctg gaaggtgggc gccacgtggt cgcgaccgta cgggccctgg tcgagcgcgg
     1081 aattccggtc atggggcatc tggggctcac gccgcagtcg gtgcacgccc tgggtggcca
     1141 ccgaatacag gggcgggaga ccggagcgcc ggagcgcatg cgcgacgatg cccgtgagct
     1201 ggaggatgcg ggtgccttcg ggttggtcct ggaactggtg ccggccgggc tggcccggcg
     1261 gatcagcgag acgcttcgga ttcccaccat tggcattggc gcgggggccg ggtgcgacgg
     1321 tcaggtcctg gtgctgcacg acatgcttgg cctcaatgag accttcagcc cccggttcct
     1381 caaacgtttc gcctcgttgg cgggcactgt ccgcgacgcg gcgagagcct atgcgaagga
     1441 ggtccgggca ggcacttacc cggacgacca acacagtttt gaatgacggc gatgctcgag
     1501 atcagcgaga tcggcgctct ccgccattgg cgcgacgatc agcggcatgc cggtcaccgc
     1561 gttgccctgg ttcccaccat gggatcgctg cacgaaggtc acctggcgct ggtggatgag
     1621 gcgcgtcggc gtgccgacgc cgtggtgctg agcgtcttcg tcaacccgct ccaattcgga
     1681 cccgaagaag atttcgagcg atacccgcgc aatctcgagc gcgaccgggg tctggcggca
     1741 gggcgcggcg ttgatcttct ctttgcaccc gaaaccgaag ccctctaccc gcagggctcg
     1801 gagatccgga ttgtgcccgg tgagacggcg gagcggtggg aggggagcat ccgcccgggg
     1861 cattttgcag gtgttctcac tgtggtcgca aagctcctgc atctggtgga gccggacgtg
     1921 gcctgcttcg gacaaaaaga cattcaacag gtcacgctgg tccggcgaat gatccgcgat
     1981 ctcgactggt cggtcgagat tgccgtggta cccactgtgc gcgaggccga cggcctcgcc
     2041 atgagcagcc gcaatatcta cttgagccca gctgaccggg ccaaggcgct gtcgttgagc
     2101 cgcgggcttc gggctgcgag agatgcttgg cagggcggtg agcgctccgc cagcgtgctc
     2161 aagcgcatcg tgcagtcgag tcttcgcgct gctacggggg ttcgtgccga gtatattgcc
     2221 gtagtcgagc cggagaaatt gcgaccggtc gcggttgcac cggcgggaac cattctcgct
     2281 gtcgcagccc gggtcgggaa cacgcggctc atcgataacg tgatcctgac gtaggagtgc
     2341 aagtgcagcg ccacctgatg aaatcaaaaa tccaccgtgc aacgatcacg gcggcggatc
     2401 tccattacga gggctcgttg acagtggacg aggatctgct ggacgccgca gatctggtga
     2461 cccacgagga agtccaggtc gtcaacgtga acaatggttc ccggttcacg acctacgtta
     2521 ttcccggccc tcgcggctcc ggcgtcatgc agctgaacgg cgcagccgct cgactcggcc
     2581 acccaggcga cgtggtcatc ctgatctcct atggcgtgtt taccgatgct gaggccaagc
     2641 ggcacgtgcc acgcgtggtc ttcgtcgatg aacggaaccg cttggtgcgg accccgttgc
     2701 gggcggagag ctgagcaccg acggggggtc cgaccctctc gtgtcgtcgg cccgtcaaac
     2761 gccgcccggt cttcggccaa gcttgccgac ctgggcggtt ctttcatctg agcgattggc
     2821 gcatgtgcaa cgggtcgcgg aactcgccgc ttcctgggcc gaggcgatgg gagtgccgga
     2881 caacgagcgg catcgctggc tgcgagcagt gtggttgcac gatgctttga gggatgcgcc
     2941 ccccgaagag ctggaccgct gggcatcgtc ggcgccggtg ccggccagcc tcaagcacgg
     3001 accggccgcc gccgcacggg ccaaggctga gggtgaggta gatcgcggcg tgctggacgc
     3061 tgtgcggtat cactcggtcg ggctggcgga gtgggatatg gtcggacgag tcttgtacgc
     3121 ggccgattat ctggagccgg gccgttcctt cgaccgggag cgacgggccg aattggctcg
     3181 ccgctttccg gcggattcac gcggtgtgct tcgcgagatt gccggtgagc gtctagcgca
     3241 tatcgtggca tccggctggc ccctgcccga acccacggtg cggttttgga actctctcgt
     3301 caccccgtcg tcatcgcgct gatcgttctc ggcgtattgg gtgggaccgt cgccgccgtt
     3361 gcgctgcggc cgcccctgcc ggcgccgacg ccgatcaaac atgcttacgc cgtgcccgcg
     3421 cgggagcatc gcattctggt cgaggttctg aacactacca cgcgtcccgg cctggcccgg
     3481 tccgcaacca gagtgctccg acggcagagt ctcgacgtgg tcttctttgg aaatgccgat
     3541 ggcagcgcca aggtcgactc gacgcaggtg atcgcccggc gaggcaaccg tggtgcggct
     3601 cagacggtgg cgaaggtcct ggggcagggt gtggtgaagc ttcagaccga cacccttcgg
     3661 agggtagatg tgacggtact gctggggagc gactatcggc cgccggtaga tggacacccc
     3721 tgaaagcaaa agcgtgaacg gtgaacggga gccttcttga tcatcggagc tcccgttcac
     3781 cgtttaccgt ttacctttca cgcttctcct caaaggccac ctctcctccc accaccgtca
     3841 tcacacacct tgttttgaga atttcccgtt cgggaatttg catgatgtcg gcggagagga
     3901 cggtgaggtc ggcccatttg cccacctcga tcgagccgcg ttgctgttcc tggaacgcag
     3961 caaatgcagg ccacagcgtg agcatcttga gtgcttcccc ccgggtcaag gcttgctcgg
     4021 gatgccagtc cggtccccga aagccactgg tgtctttccg agcgacggct gcgtagaact
     4081 cgatcatcgg ctcacccagc tcgaccggtg cgtcggagcc accgggaatc ggaacaccgg
     4141 t
//
LOCUS       DASRJQ010000466         8836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3678492, whole genome shotgun sequence.
ACCESSION   DASRJQ010000466 DASRJQ010000000
VERSION     DASRJQ010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 8836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8836
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3678492"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VFB89_10505"
     CDS             <1..801
                     /locus_tag="VFB89_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27779.1"
                     /translation="GCVSVAGCSSEPVNPSPYTVDAFISAVSARNGAVVATMHETSPP
                     PAGSGPTAQVSGITTAVNGGSAPVNLTAGGTFNRVYVSTPNAQGYWEVLLPSGSTAED
                     LIVSVARGVRSGSLRVRYQAENAGVAGPYAEQTLQIIGVGTGDVQVSIAWTGATDVDL
                     HVIDPNGEEIYYAHRTSASGGQLDLDSNPACSIDNKNNENIVFPTGTAPHGSYRVMVY
                     YYAACQQPRSDWVVTTLVKGQQPQTFTGSFVGPEGTGNPPIQATTFTY"
     gene            complement(798..1241)
                     /locus_tag="VFB89_10510"
     CDS             complement(798..1241)
                     /locus_tag="VFB89_10510"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZM27780.1"
                     /translation="MTEPGPQPPGVLETALYCPDLDAAERFYRDVVGLQVIGRQTGRH
                     VFLRCGEGMLLLFNPEATADPTNAADGPAVPRHGAHGPGHVAFRLPERDIAGWLAQLS
                     ASQVPIEAEIHWPQGGYSLYVRDPAGNSVEFATAAVWGIPEPPRR"
     gene            complement(1308..1652)
                     /locus_tag="VFB89_10515"
     CDS             complement(1308..1652)
                     /locus_tag="VFB89_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HZM27781.1"
                     /translation="MTSTTFDLPGYRVVQSLGLVRGVTVRSRSIIGTIGAKIETIFGG
                     HILTLTSLCETARADAFEILRAHAQRRGANAIIGVRYDAAEVMAGVTEVLCYGTAVTV
                     EPLESGGDERLA"
     gene            complement(1690..2337)
                     /locus_tag="VFB89_10520"
     CDS             complement(1690..2337)
                     /locus_tag="VFB89_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27782.1"
                     /translation="MDAAPSRYRRIIGGTSLIVGPALMSIGDLFHPAEHPGAATQVAI
                     IVHSASRWYAAHLLLFVGLLLLVPGFLAITKVAMDRRPGAGYAARVLMLAGVAAFSAV
                     IAFEMLLGRFIAERNDQAAATALLDTFQSPEIWMALVPGLLAFFVGTALAVVSLASAG
                     DPFRWPALAYAVGASLILGEIILAKVLLSQIGNIIMLVAGTAFARLLLRRREGAA"
     gene            complement(2382..2591)
                     /locus_tag="VFB89_10525"
     CDS             complement(2382..2591)
                     /locus_tag="VFB89_10525"
                     /inference="COORDINATES: protein motif:HMM:NF023510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3565 domain-containing protein"
                     /protein_id="HZM27783.1"
                     /translation="MIPGRRIVGFHQDAETQWVAELDCGHTQHVRHTPPWEVRPWVLT
                     EAGRAAHLGTVLSCPLCLDTPESIR"
     gene            complement(2817..3233)
                     /locus_tag="VFB89_10530"
     CDS             complement(2817..3233)
                     /locus_tag="VFB89_10530"
                     /inference="COORDINATES: protein motif:HMM:NF040341.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6174 domain-containing protein"
                     /protein_id="HZM27784.1"
                     /translation="MRPLRQRLAALSTLAVALVACSDATGPTPEKVVAHCANWLAHNL
                     TEYTYIYETRGFFISWEAQPIRVAVRNGVVVSATFVTTGEPASASPTDFPTIDKLFDQ
                     AADAAREHTLQAISFDGQLSYPRRMDLDGPRMHGAP"
     gene            complement(3259..3627)
                     /locus_tag="VFB89_10535"
     CDS             complement(3259..3627)
                     /locus_tag="VFB89_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27785.1"
                     /translation="MKTEIGRAALKGAKVGLLAGLCLSAPYLAIGALGDTELNKSLGY
                     SLLLVGFPTLFAIVPAIEWLGVQGGTREGVLLLLLTLSLNGVLWGTVVGTVVTLASMG
                     RRKWLRDARPLCRRGNAGPS"
     gene            complement(3624..3887)
                     /locus_tag="VFB89_10540"
     CDS             complement(3624..3887)
                     /locus_tag="VFB89_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27786.1"
                     /translation="MPAKLQIGDCVRVPDGRIGRVRARVGRKLRVRVRRHTSATHQFL
                     LLPPGEVRRVACPRGWMSPAGYRRYLRPTLAKMRARARTRSRL"
     gene            complement(3895..4044)
                     /locus_tag="VFB89_10545"
     CDS             complement(3895..4044)
                     /locus_tag="VFB89_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27787.1"
                     /translation="MRRITPPTDDQLMTTVEARVLRAHAHFDDAWSAPGTAWTMELDA
                     LFICK"
     gene            complement(4116..4364)
                     /locus_tag="VFB89_10550"
     CDS             complement(4116..4364)
                     /locus_tag="VFB89_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27788.1"
                     /translation="MRQVISVLGAVCILLPFAASQLGRLQTTALAYQLLNLIGATALT
                     VVAVLEHQYGFILLEGTWALVSLVGLVRVRSVSGRDAA"
     gene            complement(4488..5024)
                     /locus_tag="VFB89_10555"
     CDS             complement(4488..5024)
                     /locus_tag="VFB89_10555"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZM27789.1"
                     /translation="MPHVIVTGGPGVGKTTLLAELAAVGYATVAESARAIIAERLASG
                     ANRRPDVLAFAREILRRDIEKYLEQPRTSSWVFFDRGLIDALGMLHEASALPSVELES
                     MLALYPFHATVFVVPPWELIYTNDTERDQSFAEAVPVYTRIVRWYRSCGYVLDEVPRL
                     PVAQRAEHVLRILAESAA"
     gene            complement(5079..5528)
                     /locus_tag="VFB89_10560"
     CDS             complement(5079..5528)
                     /locus_tag="VFB89_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27790.1"
                     /translation="MSLQHLSRRGWQLAAVLAVVACGLEHDRSESKDPSEPKGPKHEA
                     QAWLRANQNPSPLASNRFGPKQRAVAFIDSVYQLGAETVYVVNVEEDSSWILQEGGPY
                     ADALWIRLPSDPALRAGLFAVDEREARGEGFDPDRDHGQQYLYFWWD"
     gene            complement(5585..6055)
                     /locus_tag="VFB89_10565"
     CDS             complement(5585..6055)
                     /locus_tag="VFB89_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27791.1"
                     /translation="MNVARCAGWFLLPLVSGACASHSAEAGAPAPSFATTADALSCAK
                     KALADVGFHVETRMEDPPGQRSAAAAVETPTSVTARIASDQQIDYIRAAAHFRKGSAA
                     DSTATLTVSAGTIIPEEGSSAWTWRALSSVALRGRDAVVGQCHAKIDQTYPGSS"
     gene            complement(6164..6640)
                     /locus_tag="VFB89_10570"
     CDS             complement(6164..6640)
                     /locus_tag="VFB89_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27792.1"
                     /translation="MCFVAASAQAQAPAQQQASTTTASPATVEQTLKALREDMQSSRA
                     DIMAKNMSLTAEQAAKFWPVFNANQKEQNAIMDDQLKGIQKYVDSYETLDDATALSLM
                     RAHMDRDAKMNALRQKWLGEFQKVLPTKLAVRAMQIDRRLSLAAQMEIASRIPLVH"
     gene            complement(6716..7156)
                     /locus_tag="VFB89_10575"
     CDS             complement(6716..7156)
                     /locus_tag="VFB89_10575"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZM27793.1"
                     /translation="MHRFEIEEPSSADIEFLEERLYEFNAGTTGINDGLSLGIFLRDE
                     SRNIVAGAAGHTWGETCELRQVWVEASARGRGLGRRLVAEVETEAVRRGCRQLVLSTH
                     SFQAPDFYRKLGFDVVLELPDYPRGHSHLVMRKRLAIAGVGPSP"
     gene            complement(7161..7385)
                     /locus_tag="VFB89_10580"
     CDS             complement(7161..7385)
                     /locus_tag="VFB89_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27794.1"
                     /translation="MPIGWGTELGYAAVRRRPGGERIPAWLLCSAALPTAWDRLDAFE
                     GAAYQRLLAPFETEAGIVAVGYFYAHTAEA"
     gene            complement(7389..7607)
                     /locus_tag="VFB89_10585"
     CDS             complement(7389..7607)
                     /locus_tag="VFB89_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27795.1"
                     /translation="MDRLSFQSLDAILQELNADLAGPAGPARAQAGERAATLFGTSER
                     LIVYGSLAPGRANYHHVGRRLDLGLDYR"
     gene            complement(7632..7910)
                     /locus_tag="VFB89_10590"
     CDS             complement(7632..7910)
                     /locus_tag="VFB89_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27796.1"
                     /translation="MSLFIEYPILAGGLGVLGLLLYAKSRRLSIGVAALAWLVYAAYE
                     AAMHLRWLCTGECNIRVDLLLIYPVLLLLSGVAAFSFARWTVQQRRNL"
     gene            complement(7926..8336)
                     /locus_tag="VFB89_10595"
     CDS             complement(7926..8336)
                     /locus_tag="VFB89_10595"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZM27797.1"
                     /translation="MKITVETSVKAKLNKVWDAWNNPADIQQWNTAQDDWHTTLSSVD
                     LREGGKFRSRMEAKDGSEGFDFEGTYTRVVPRKTIEYRMSDDREVKVEFVERAGSVLV
                     RETFDAETENPPELQRTGWQAILDNFGRHVEAKG"
     gene            complement(8361..>8836)
                     /locus_tag="VFB89_10600"
     CDS             complement(8361..>8836)
                     /locus_tag="VFB89_10600"
                     /inference="COORDINATES: protein motif:HMM:NF021425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HZM27798.1"
                     /translation="IALLKFAKAALLVAIGLGALGLLDPHMATQAQQWATAMAMSSDR
                     QLVQWLVVHAVSLPPARLEELGIGAFFYAGLFGAEGVGLWMGRRWAEYLTIVATGSFI
                     PLEAVELARRVTAVRAGALILNLAVVIYLIAHLRRSRPHGRPAGVDLAPPHSTKG"
BASE COUNT         1698 a         2826 c         2800 g         1512 t
ORIGIN      
        1 ggctgcgtca gcgtggccgg gtgctcctcc gagccggtaa acccctcgcc ttatacggtg
       61 gacgcgttca tcagcgccgt cagcgcccgc aacggagcgg tggtggcaac gatgcacgaa
      121 acctcgccac cgcccgccgg atcggggccg acggcgcagg tctcggggat cacgaccgca
      181 gtgaacggcg gtagcgctcc agtcaatctc accgcaggcg gcacgttcaa tcgggtgtac
      241 gtctccacgc cgaacgcgca gggatactgg gaagtgctgc tccccagcgg gagcacggcc
      301 gaagatctga tcgtcagcgt tgcgcgcggt gtgcgctcgg gctcgctacg ggtccgctac
      361 caggccgaga acgcgggcgt cgccggacca tacgcggagc agacgctgca aatcattggc
      421 gtcgggactg gcgacgtgca ggtcagcatc gcctggaccg gcgcaaccga cgtggatctc
      481 catgtgatcg atccgaacgg cgaggagatc tactacgctc acaggacgtc agcgagcggc
      541 gggcagctcg atctggactc gaacccggcg tgcagcatcg acaacaagaa caacgaaaac
      601 atcgtcttcc ccaccggaac ggcgccgcac ggctcgtacc gcgtgatggt gtactactac
      661 gcggcctgcc agcagccgcg gtcggactgg gtggtcacga cgttggtgaa gggccagcaa
      721 ccgcaaacct tcaccgggag cttcgtcgga cccgaaggca ccggaaatcc accgatccag
      781 gctaccacgt tcacgtacta acgcctcggt ggctcgggga tcccccacac tgccgccgta
      841 gcgaactcga cactgttgcc cgctggatcc cgcacgtaca gggagtagcc gccctggggc
      901 caatgtattt cagcctcaat cggcacctga ctcgcgctta gctgggcgag ccaacctgcg
      961 atgtcgcgct ccggaagccg gaacgccaca tggcccggcc cgtgcgcgcc gtgccgcggg
     1021 accgcaggtc catccgccgc attcgtcggg tccgcggtag cctccgggtt gaagaggagg
     1081 agcataccct ctccgcagcg gagaaagacg tgccgcccgg tttgcctgcc gatgacttgc
     1141 aggccaacga cgtccctata aaagcgttcc gcggcgtcaa ggtcgggaca gtagagggcc
     1201 gtctcgagca cgcccggcgg ttgcgggccc ggctcagtca ctctcgcgct catctctccg
     1261 ggccaacgtc ccgctccatc tggagctcga tgtccccgtc acgtgggtca agcaagacgc
     1321 tcgtccccgc cactctccaa cggctcgacg gtgacggccg tgccatagca aagaacctcg
     1381 gtaacccccg ccatgacctc tgcggcatcg taccgcacgc cgataatggc attggcgccg
     1441 cggcgctgtg catgggctcg caggatctcg aaggcgtcag cccgggcggt ctcgcagagt
     1501 gatgtaagcg tgaggatgtg tccgccgaag attgtctcga tcttcgcgcc gatcgtgccg
     1561 atgatggagc gcgagcggac agtcacgcct cgcaccaggc caagtgattg tacgacccga
     1621 tatcctggga ggtcgaaggt cgtggaggtc atcgcttcgt ccatagtaag tccccatgtg
     1681 ttgcgtgggt caggcagccc cctctctacg ccggagcagc aaccgtgcaa acgcagtacc
     1741 ggcaacaagc atgataatgt ttccgatctg gctgaggagc actttagcca ggatgatctc
     1801 gcccaggatc aggctggcgc cgaccgcgta ggcgagtgcc ggccagcgaa aaggatctcc
     1861 agcggacgcg agcgaaacga cagccagcgc agtgccgacg aagaacgcga gcaggccagg
     1921 cacgagcgcc atccagatct cgggcgattg gaaggtgtcg agcagagcag ttgcggccgc
     1981 ctggtcgttg cgctcggcga tgaagcgccc gagcaacatc tcgaaagcga tcaccgccga
     2041 aaacgcggcc accccagcga gcatgagaac gcgcgcagca tagcccgccc caggccggcg
     2101 atccattgcc accttggtga tggccaggaa gcccggcacc agcaggagaa gcccgacaaa
     2161 caagaggaga tgagcggcgt accatcgcga ggccgagtgc acaatgatcg cgacctgggt
     2221 agccgcccca gggtgctctg cgggatggaa caggtcgccg atgctcatca gcgccgggcc
     2281 cacgatcagg ctcgtgccgc cgattatcct gcggtacctg gagggggcag catccatggg
     2341 atacctccct attaggcgaa ccaacgtgcg ctctagcgtc tctaacgaat tgattcagga
     2401 gtgtccagac agagcggaca ggacagcacc gtaccaaggt gggctgcccg acccgcctcg
     2461 gtcagcaccc agggccgcac ttcccacggg ggcgtgtgtc ggacgtgctg cgtgtgcccg
     2521 cagtcgagtt cagcgaccca ctgggtctcg gcatcctggt ggaagccaac gattcgccga
     2581 cccggaatca ctcccgacct ccgcaacgac caacgaacgg accgatcaaa tctagagctc
     2641 cgtcaccgtg ttccgcaggc tgtcggcctg ggcacctgtg ccgtctgggc gccccgccac
     2701 cagtgaacga tggcttcctt actgcccggt ccgtaagcac taatctgacg gatggcgcat
     2761 atgcagttcg agagtcgtgc cggtcgctca gggagccggc tggacctggg tagcgatcaa
     2821 ggagccccgt gcatccgggg gccgtcgaga tccatgcgtc gcgggtagct cagttgaccg
     2881 tcgaagctga ttgcctgcag agtgtgctcg cgagcggcgt ctgcagcctg atcgaagagc
     2941 ttgtcaatgg ttggaaagtc ggtgggtgat gccgacgctg gttcaccagt agtgacaaac
     3001 gtggcggaca ctacgacacc attgcggacc gcaacccgaa tcggctgagc ttcccagctg
     3061 atgaaaaatc ctctggtctc atagatgtag gtgtattcag tcaggttgtg agccaaccag
     3121 ttcgcgcaat gggcaacaac tttctctggc gttgggcccg tggcgtcgct acaggccaca
     3181 agcgcgacgg caagagttga cagggctgca agtcgctggc gtagtggacg cacggccgct
     3241 ctgttgcaga ctctatcact aggacggacc ggcgttgccg cgcctgcaca acggtcgcgc
     3301 gtccctgagc cacttccttc gccccatcga cgccagtgtc accaccgtcc cgacgaccgt
     3361 gccccaaagc acgccgttca gcgacagagt cagcaagagt aggagcacgc cctcgcgcgt
     3421 accgccctga actcccaacc actcgatagc gggtacgatt gcgaagagcg tggggaagcc
     3481 gacgaggagc agcgagtagc cgagcgactt gttgagctcg gtgtccccaa gagcgccgat
     3541 cgccaggtac ggtgcgctga ggcacagccc ggcgagcagc ccgacctttg cgcccttgag
     3601 agctgcgcgc ccgatctccg tcttcacaag cgactccggg tccgcgcccg agcgcgcatc
     3661 ttggccagag tgggtcgtag gtaccgacga tagccggctg ggctcatcca gccacgcggg
     3721 cacgccaccc tgcgcacctc gccaggaggg agcagcagga actgatgtgt ggcgctcgtg
     3781 tgcctgcgca cccgcacccg gagcttgcgt ccgactcgag cgcgcactcg tccgatccgg
     3841 ccgtccggca cccgcacgca atcgcctatc tggagcttcg ccggcatgca agactcactt
     3901 gcaaatgaat agtgcgtcga gttccatggt ccacgccgtg cccggggcgc tccaggcgtc
     3961 gtcgaagtgc gcgtgcgccc tgaggacccg ggcttccacc gtcgtcatca gctgatcatc
     4021 cgtcggcggt gtaatgcgcc gcacgaccgc ctcgagctgc agtggacgct ctgccagtcg
     4081 aggtgttctc ggggctgtct gcggcagtct cgggctcagg cggcatctcg cccggagact
     4141 gaccgtacac gaaccagtcc caccaggctc accagtgccc aggttccctc cagtagaatg
     4201 aagccgtact gatgctcgag gacggcaacg acagttaggg cagtcgctcc aatgaggttc
     4261 agcagctgat atgccaacgc cgttgtctgc agacgaccca gttggctcgc ggcgaacgga
     4321 agaaggatgc agactgctcc cagcaccgaa atgacttggc gcatagcgtt ggtccgatca
     4381 agagagctgt ttgatgccgc ttcgggcctg ggccgtcaca acgaaagaga ccatgctggt
     4441 ttccccccgt cgaaggggtt aggggtcacg ccccagcatt agaaagctca ggcggcgctc
     4501 tcggcgagaa tgcgcagcac atgctcggct cgctgtgcca caggaaggcg gggtacttca
     4561 tcgagaacgt agccgcacga acggtaccaa cggacgatcc tggtgtagac gggaacagcc
     4621 tcagcaaacg attggtcgcg ttctgtgtcg ttggtgtaga taagctccca cggcggcacc
     4681 acgaacacgg tggcatggaa cgggtagagc gcgagcatgg attccagctc gacggaaggc
     4741 agagcggatg cctcgtgaag catcccaagg gcatcgatca ggccgcgatc aaagaagacc
     4801 cagctggagg tgcgaggctg ttcgaggtac ttctcgatgt ctcgacgcaa gatctctcgg
     4861 gcgaaagcaa gtacatccgg tcgacgattc gcaccggacg caaggcgttc tgcgatgatg
     4921 gctcgagcgg attctgcgac tgttgcgtag cccacggcgg caagctcagc caggagcgtc
     4981 gtcttgccca cacccgggcc gccggtgacg atgacatgag gcatcgatcc ctgaggtcag
     5041 cgaccgctga cagtccgttt cagctgcggc atggcgcgtc agtcccacca gaagtacagg
     5101 tactgctgtc cgtgatcgcg atccggatcg aagccctcac ctcgggcctc tcgctcatcg
     5161 acggcaaaga gaccagcccg gagcgccgga tcgctgggca accggatcca tagcgcatcg
     5221 gcgtagggcc ctccttcctg cagaatccag ctggagtcct cctccacgtt gacgacgtac
     5281 accgtctcgg ccccgagctg gtagacggag tcgatgaacg caaccgccct ctgtttggga
     5341 ccaaatcggt tagaagccag gggcgagggg ttttggttcg cccggagcca ggcttgcgct
     5401 tcatgcttgg gtcccttggg ctcgctggga tccttggact cggagcggtc gtgttcgaga
     5461 ccgcaggcga cgacagccag cacggcagcg agttgccaac ctcgtcttga aaggtgttgg
     5521 agagacatga ctcgcttccg aaactggtcg agggcttggg gaatgagccg ttgcggcgcg
     5581 attctcagga gcttccggga taggtttggt cgatcttggc gtggcattga cccacaaccg
     5641 catcccgccc ccgcagggcg acgctggaga gcgcgcgcca ggtccacgcg ctactgccct
     5701 cctcgggtat gatggtcccg gcactgacgg taagcgtagc tgtgctatcg gcggcggacc
     5761 ctttccgaaa gtgggcggcg gccctgatgt agtcgatctg ctgatcactg gcgatccgcg
     5821 cggttacaga cgtaggcgtc tccaccgcgg cagcagcgga tcgttggcca ggcgggtctt
     5881 ccatcctggt ctcaacatgg aacccaacgt cggcgagcgc cttcttggca cagctgaggg
     5941 cgtcggcggt ggtggcgaag ctcggcgcgg gcgccccagc ctcggcggag tgtgacgcgc
     6001 aagctcccga aaccagggga agcaggaacc acccagcgca acgggccacg ttcaagagcg
     6061 ctccgaatcc aagaacgtca accggaacct gacatgtccg aggccgccgc acgagggcgc
     6121 gattgcgtca gccaggggcg gtttgcgaca gttgtgcgct ggctcagtgc accagaggaa
     6181 tacgcgacgc gatttccatt tgggccgcca aggatagccg gcggtcgatc tgcattgctc
     6241 gcacggcgag cttcgtgggc aacaccttct ggaattcacc cagccacttc tgacgcaggg
     6301 cgttcatctt ggcgtcccgg tccatgtggg cgcgcatgag tgagagcgcc gttgcgtcgt
     6361 ccagggtctc gtagctgtca acatacttct ggatgccctt gagctgatca tccatgatgg
     6421 cgttctgctc cttctggttg gcgttgaaaa ccggccagaa ttttgccgcc tgctcggcgg
     6481 taagcgacat gttctttgcc atgatgtccg ctctgctgct ctgcatgtcc tcgcgcaagg
     6541 ctttcagggt ttgctccacc gtcgccggtg acgccgtggt ggttgaggcc tgctgctgtg
     6601 ccggcgcctg ggcctgcgcg gaagctgcga cgaaacacac gaccaagagg ggtgtcagag
     6661 tccggtgcat cggtatttct ccagggtgga aaggggagca aggttgtcat gaagcctacg
     6721 gcgacgggcc cacgccggca atcgcaaggc gcttgcgcat caccaggtga gaatgccccc
     6781 gggggtaatc gggaagctct aaaaccacgt cgaagccgag cttccgatag aaatcgggcg
     6841 cctgaaaact gtgcgtcgag agaacgagct gccggcagcc gcgtcgcact gcttccgtct
     6901 cgacctcggc aacgaggcga cggccaagac cccggccgcg ggcggacgcc tccacccaca
     6961 cctgacgcag ctcgcaggtc tctccccacg tgtggcccgc ggcacctgcg acgatgttcc
     7021 tggattcgtc tcgcagaaaa attccgagac tcagaccgtc gttaatacca gtcgtaccag
     7081 cattgaactc gtacagtcgt tcctcgagaa actcgatgtc ggcggaagat ggttcttcga
     7141 tctcgaagcg atgcatccgg ttaggcctca gccgtatgtg catagaagta gccgaccgca
     7201 actattcctg cctcggtttc aaaaggggcg agcagccgct ggtacgccgc cccctcgaag
     7261 gcgtcgagtc gatcccacgc ggtcggcaag gcggcggagc agaggagcca cgccgggatg
     7321 cgctcgccgc ccgggcgtcg acgaaccgcg gcgtagccca gttccgtgcc ccacccaatc
     7381 ggcaccggtc accggtaatc cagcccgagg tccaatctcc gcccgacgtg gtgatagttc
     7441 gcacggcccg gcgccaagct tccgtacacg atcagacgtt ccgaagtgcc gaagagtgtg
     7501 gcggcgcgct caccggcttg cgcccgcgct ggcccggccg gcccggcgag gtcggcgttg
     7561 agctcctgca agatggcatc gagcgattga aaggacagac gatccacggc gccgccgatt
     7621 gctagggtcg atcaaaggtt gcgccgttgt tgaaccgtcc accgcgcgaa gctgaacgcg
     7681 gcgacgccag agagtagcag gaggacggga tagatcagga gcagatcgac ccgaatattg
     7741 cactctccgg tgcagagcca acgcaggtgc atggctgcct catatgcggc gtagaccaac
     7801 catgccaacg cggcgactcc gatactgagc cttcgggact tggcgtagag cagcaatccc
     7861 aatacgccga ggccgcctgc aagaatcgga tactcgatga atagactcat ccgagcggca
     7921 gcggtttagc cctttgcttc cacgtgccgg ccgaagttgt ccaaaatggc ctgccagccc
     7981 gtgcgctgga gctcgggtgg gttctcggtt tccgcgtcga acgtctcccg caccaggacg
     8041 ctgccggcgc gctcgacgaa ctcgaccttg acctcccggt cgtcgctcat gcggtattca
     8101 atggtcttgc gcggcacgac gcgcgtgtac gtgccctcga agtcgaagcc ctcgctgccg
     8161 tccttggctt ccatgcgcga ccggaacttt cctccttcgc gcagatcgac ggaactcaag
     8221 gtcgtgtgcc agtcatcctg ggcggtattc cactgctgga tgtccgccgg attgttccag
     8281 gcatcccaga ccttgttcag cttggccttt acggatgtct cgacggtgat cttcatgggt
     8341 gtctccgacg tatagggtgg tcaccccttc gtcgaatgag gaggggccaa atcgacaccc
     8401 gctggtcgtc cgtgggggcg agagcgccgc aggtgcgcta tcaagtagat gacgaccgca
     8461 aggttcaaga tcagagcgcc tgcccgcacc gcggttactc gccgcgcgag ctcaacggcc
     8521 tctaatggta tgaacgaccc ggtagcgaca atcgtcaggt actcggccca ccgccgtccc
     8581 atccagagcc caacgccctc cgcgccgaag aggcccgcat agaagaaagc accgatgccc
     8641 agctcctcta gccgagcagg cggcaggctc acggcatgca ctaccaacca ctgaaccagc
     8701 tggcgatccg agctcatggc catggcagtt gcccactgtt gtgcctgcgt ggccatatgc
     8761 ggatccagga gcccgagggc acccagaccg atggcgacca gcaacgccgc cttggcaaac
     8821 ttgagcagcg cgatga
//
LOCUS       DASRJQ010000467         2232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2938724, whole genome shotgun sequence.
ACCESSION   DASRJQ010000467 DASRJQ010000000
VERSION     DASRJQ010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2938724"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..840)
                     /locus_tag="VFB89_10605"
     CDS             complement(163..840)
                     /locus_tag="VFB89_10605"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HZM27799.1"
                     /translation="MKLHSRSFMALLLGAGLFAGSASHAGAIAGKGIRAGLAVANLPG
                     DFADLMGTDWRVGFAGGGFLRINVPGGVGLQTELLYAMKGATTHVDLTDQSGNTVGTA
                     TTTYQVDYLEIPVLLRVTVGGPGPVQPSLLLGPSLGVKLRSRLAYDGPGPDMSGDLKV
                     EDTDFGLVGGVGLEFGHTPARMLLDARYTLGLMNVYALELMTFTGPPSVLNARTGTFT
                     ITAGLVF"
     gene            complement(911..2029)
                     /locus_tag="VFB89_10610"
     CDS             complement(911..2029)
                     /locus_tag="VFB89_10610"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZM27800.1"
                     /translation="MAHMRFIGHELIAALFMALPPACAVAAAPPSAGVFESAAAQTVD
                     GHATGAILVASHGRILWTGSVGDAKKGIPPPSPDAVFDALSIGKTFTAIAVQRLVFTG
                     KIDLEASIGRYIPELPKQLDSITVQNCLDNASGWGPYLNDKGDFDPESTAQLIEDLAK
                     AERKGRVGEYSYSNTGFQALGLVVQRVTGKPFKEAMRDLVFRPAKLLSTDFLGLPPLR
                     ERPTVIGWRDGRGTGSARTWPSSWSLMGAAGIAATVDDLFRLNRTFIAGDGLGAAGRA
                     RMLADGVPTGGRAPYREPGRTMISYGSGLYHWRDAQGRRVHFHGGDGDYGFHAAMFWR
                     EDDDLFIVGLFNSGGPAGDFDRAAFMNAFAEAAKQVTR"
BASE COUNT          407 a          742 c          692 g          391 t
ORIGIN      
        1 ggccgcctac gcggcgagcg cgtgccggag ggcgtcccac ccgcggaacc gggcaaggag
       61 ccgaaccgag ttcgtaacga atcccgttgg ggacgctcct tcgccggcgt gggcaagccc
      121 ccgctccatg ccgtgaaagt cggcgaggcg gccctaccgg gctcagaaga caagccccgc
      181 ggtgatcgtg aacgtgccgg tgcgtgcatt gagaacgctc gggggccccg tgaaggtcat
      241 cagctccagc gcgtagacgt tcatcagccc cagcgtgtag cgcgcatcca gcagcatgcg
      301 cgcgggcgtg tgaccgaact cgagccccac gccaccgacg agaccgaagt cggtgtcctc
      361 gaccttcagg tccccgctca tgtccggacc ggggccgtcg taggcgaggc gactcctgag
      421 cttgacgccc agcgaggggc ccagcagcag gctcggctgc accggtcccg ggccgcctac
      481 ggtcacgcgg agcaacaccg gaatctcgag gtagtcgact tgataggtgg ttgtggcggt
      541 gccgaccgtg ttgcccgact ggtcggtcag atccacatgg gtcgtcgcgc ccttcatcgc
      601 gtagagcagc tcggtctgga gtcccacccc tcccgggaca ttgatccgga ggaatcctcc
      661 cccggcgaag ccgacacgcc agtccgtccc catcaggtcg gcaaagtctc cgggcaggtt
      721 ggccaccgcg agcccagccc tgatcccctt ccctgcgatg gctcccgcgt ggctcgcact
      781 gccggcgaac aagccagcgc ctaggagcag agccatgaat gatctgctat gcagcttcat
      841 tgcggactct ccttcccttc ccgcgcgggt ccggcccgcg gcatccttga acaacctcct
      901 gagctcggcg ctagcgggtc acctgtttgg cggcttctgc aaaggcattc ataaacgccg
      961 cgcgatcaaa gtcgccagcg gggccaccgc tgttgaacag cccgacgatg aacaaatcgt
     1021 cgtcctcccg ccagaacatc gctgcatgaa atccgtaatc gccgtctccg ccatggaaat
     1081 gcactcggcg tccttgcgca tcgcgccaat gataaagtcc cgagccgtag ctgatcatcg
     1141 tgcgaccggg ctctcggtac ggcgctcggc cgcccgtcgg cacgccatcc gcaagcatcc
     1201 tggcgcgccc tgctgcgccg aggccatcgc ccgcaatgaa ggtccgattg agccgaaaga
     1261 gatcgtcgac agtggctgcg attccggccg cgcccatcag cgaccaactc gaaggccatg
     1321 tccgcgccga accggtgccc ctgccgtccc tccagccgat gaccgttggg cgctcacgca
     1381 atggcggcaa gccgaggaaa tcggtcgaga gcagcttcgc tgggcggaag accagatcgc
     1441 gcattgcttc cttgaacggc ttgccggtga cccgttgcac cacgaggccg agcgcctgga
     1501 accccgtgtt cgaataggag tattcgccca cccggccctt tcgctcagcc ttcgcgagat
     1561 cttcgatcag ctgtgccgtg ctttcgggat cgaagtcccc cttgtcgttg aggtacgggc
     1621 cccagccgct cgcattgtcc agacagttct ggactgtgat cgagtcgagc tgtttcggca
     1681 gttcgggaat gtagcggccg atcgaagcct cgaggtcgat cttgcccgta aagacgaggc
     1741 gctgcaccgc gatggcggtg aacgtcttgc cgatcgacaa tgcgtcgaat acggcatcag
     1801 gggaaggcgg cggaatgcct ttcttcgcat cgccgacgct gccggtccac aggattcgac
     1861 cgtgactggc cacgaggatc gcgcctgtcg cgtgtccgtc caccgtttgc gccgctgcgc
     1921 tctcgaaaac ccctgccgat ggtggagccg cggccactgc gcatgctggc ggaagcgcca
     1981 tgaacagggc agcaatcagc tcgtggccaa tgaaacgcat atgggccatt ctaagctgca
     2041 agcttctggc cgtcactcga agggtggcgg ggcattcatg cgcactccaa aaacccattg
     2101 cgacatgaca tctgtcgatc tagcttgcac cgattgcgta acgtaatcag aaccgcgcgg
     2161 atgtgccgta taaggaaagg aagtgggtat ggctaggctc gtgtttggaa tgaaccagtc
     2221 cctggacggc ta
//
LOCUS       DASRJQ010000468        10466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1510833, whole genome shotgun sequence.
ACCESSION   DASRJQ010000468 DASRJQ010000000
VERSION     DASRJQ010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 10466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10466
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510833"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="VFB89_10615"
     CDS             <1..1038
                     /locus_tag="VFB89_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZM27801.1"
                     /translation="PLHPRKLLAAAGFGVRALQSLTGLASRFKTKAARALLAGCGAHS
                     MIGLTEYLSGAFGLIFLASGHSVGWPIPAGGSQRIADALAAHLESLGGTIVLGQRITS
                     IDHLEPARGVLFDISPRQLLEIAGGRLSCWRGSGLYLRQLRRFRQGPGVFKLDLALDG
                     PIPWRDPEVLRAATVHLGGTLEEIVASEAAATNGRIPESPFVLLAQCSLFDSSRAPAG
                     RHTVWAYCHVPNGATVDMTERILTQIERFAPGFRDRILATSKLTPADLERYNPNNLGG
                     DIGGGRSDIGQFFTRPAWRLDPYSTPDPGIFLCSASTPPGAGVHGLCGWYAARSALRG
                     ILRGGHGTQIA"
     gene            1045..2508
                     /locus_tag="VFB89_10620"
     CDS             1045..2508
                     /locus_tag="VFB89_10620"
                     /inference="COORDINATES: protein motif:HMM:NF018471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HZM27802.1"
                     /translation="MVDWFFAPDYRIARWLIERGMAAIYLLAFACVLDQFPALLGERG
                     LLPVPQFLNGLRFRDEPTLFRLYYSDRLLRAVGWTGVLLAAILLLGLPQAGPLWLPML
                     VWLVLWLLYLSIVNVGQRFYSFGWESLLLEAGFLAIFLGNASTAPPFVIMLLFRWLLF
                     RVELGAGLIKLRGDPCWRELTCMDFHHETQPMPGPLSWYFHRLPRTLHRIEVLGNYAA
                     QLVAPFMLFLPQPVAGIGALLMIGTQSYLMVSGNYAWLNALTLVIAFAGLPDAWPSHL
                     WPGTLSAQEFAVAPYWYLVLVLLVGGLVVVLSYWPVRNLIGPGQRMNYSFNRLHLVNT
                     YGAFGSVTRERHEVVLEGTTSEKAFDSAVWQEYEFRAKPGDPRRRPPQFAPYHLRLDW
                     LMWFAALSPRYAEDWLLPLVHRLLQNDPATLRLLRRSPFTQAPPVWIRGQLYHYRFTT
                     GKERQATGAWWVRELVADYFPPVRLPPRGSTRRDTGQ"
     gene            2477..3658
                     /locus_tag="VFB89_10625"
     CDS             2477..3658
                     /locus_tag="VFB89_10625"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZM27803.1"
                     /translation="MARQGATLGSDLAERDRVGHIPSPMRKLIVLLALTGILASCRQD
                     PRPIQLGLVLTLADSGVAPMLRGARLAVSEINRAGGVRGRPLSLDVRDDFGDPDSAVR
                     VAGDLYRSDVVAVIGSAYSAPTLAAAPVYNGGRRPLIQISPSASVPDLSNAGDYTFRT
                     CPTDLEYGAALARWAHDRLGLSRAAILYVNDDYGRGFRLNFVREFERLGGVATVVAPF
                     LASDPDINAYLIRLQARHDADVLVLGANLTEGLVALQQIRASGLKLPVLAGDGFVGVE
                     TRGDVAEGLYVSTAYLVGSKRMANRRFVNAYRHEYPSAPPPDQGAASTYDVVRLLAKS
                     IQQAGADRDRIRSVIAGTGTRTPPFDAAIGPISFDSHGDVPSLGARIGVVRHGELEPA
                     E"
     gene            3730..4716
                     /locus_tag="VFB89_10630"
     CDS             3730..4716
                     /locus_tag="VFB89_10630"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZM27804.1"
                     /translation="MEQRRLGAQGLVVSAMGLGCMGMSEFYGSRDDDESVATIHRALE
                     LGITFLDTADVYGPYTNEELVGRAIRDRRDKVVLATKFGNIRGADGSFAGINGRPEYV
                     RQACDGSLKRLGVDHIDLYYQHRVDSNVPIEETVGAMAELVRAGKVRYLGLSEAGPAT
                     IRRAHAVHPISALQTEYSLWSRDPEDDVIPACRELDIGFVPYSPLGRGFLTGRFRSLE
                     DLPADDWRRNNPRFQGENFQKNLNLVTRIEDLARSKGATPSQLALAWVLGQGRDIVPI
                     PGTKRVRYLEEDVAAVHVRLTADDLAAIDAAAPAGVAAGERYPAAGMARVNL"
     gene            4733..5377
                     /locus_tag="VFB89_10635"
     CDS             4733..5377
                     /locus_tag="VFB89_10635"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HZM27805.1"
                     /translation="MKIGIIGAGRIGGTAARLFAGAGHEVVLSNSRGPASLSDLVAAI
                     NREVGEGRAQAATAADAAKFSDIVMLAAPWRADEALPDPDLVRGKIVIDAMNAYGESG
                     SVIDLGGSGSSEEVAKRLPGARVVKAFNTIYYQHLVGQGRPNAPLAERRAIFIAGDDP
                     TAKRIVSDLIRQIGFGPVDTGTLREGGRTQQPGTPVYNRDLTQAEAVAMLAGAS"
     gene            5390..6379
                     /locus_tag="VFB89_10640"
     CDS             5390..6379
                     /locus_tag="VFB89_10640"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZM27806.1"
                     /translation="MEYAQLGRSGLSVSRICLGCMSYGSPTWRPWVLDEATARPFFRR
                     AIEAGINFFDTADMYSVGASEEVTGRALRELARREEIVLATKVFFPLKPGPNQGGLSR
                     KHIMEACEASLRRLGFETIDLYQIHRFDPVTPLEETLAALDHLVHSGKVRYIGASSGY
                     AWEMALALSISERNGWARFITMQNHYNLIYREEEREMLPLCRHEGVGVIPWSPLARGL
                     LARPRPADNRLKSGGTHRAESDAYSPRLYDSAGDWDVVDAVESVAKKRGTSMAEVALA
                     WLLSRPGVTAPIVGATRPEHLEAALRAVYLELRDDERAALEAPYRPHAVRGIE"
     gene            complement(6395..8143)
                     /locus_tag="VFB89_10645"
     CDS             complement(6395..8143)
                     /locus_tag="VFB89_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27807.1"
                     /translation="MRRFVWPAWLVCLASLTPLLAAQQPKVPRAGSSFAVSFPRARSA
                     EALDGRVLLLVSTDSSKEPRFQIADGPETQLVFGVNVDRLAPGKPAVVGPAAFGYPLQ
                     RLRAIPPGRYWVQAVLNRYETFHRADGHVVKLPPDMGEGQHWEKKPGNLYSVPRWIQI
                     DPRLKIGPFPVVLDQIIPPIPAPAETRYVKHIRIQSDLLTRFWGHPVYLGAHILLPEG
                     FYDHPEARYPLILSHGHFPESIEGWRETPPDPNLAPDSSTRFRLKGYNRIQEQYAYQL
                     FKDWTGPGFPRVIVVEIQHPTPYYDDSYAVNSANNGPYGDAIMKELIPYIEQKYRGIG
                     KGWARFTYGGSTGGWEAMAAQVFYPDEFNGAWVACPDPIDFRAYTVVNIYRDTNAYRL
                     DSRWKRTPRPGERDWLGRVTATLAELNHKELALATRGRSGEQWDIWQAVYSPTDKDGY
                     PKPIWNKLTGEIDRSVAEYWREHYDLSNILQRDWKTLGPKVRGKLHIYVGEADNYYLN
                     NAVYLTEEVLKSLKDPPADAEVDYGVRAEHCWNGDHTQPNAISRLRYHQMFIPRVMDQ
                     IRKNHPPDADTTSWRY"
     gene            complement(8152..8841)
                     /locus_tag="VFB89_10650"
     CDS             complement(8152..8841)
                     /locus_tag="VFB89_10650"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZM27808.1"
                     /translation="MTSNLPPERVHVTVDIVIFTIRFDRLNVLLVRRGVPPFEGRWAI
                     PGGFVREAESLETAALRELEEETGVRDVYLEQLYTFGEPGRDPRGRVITVAYYALIAA
                     DRAAPVGGSDAAAAQWWSTAELPPLAFDHDAMLKYAIERLRNKLEYTTVGFQLLPRQF
                     TLTQLQRVYEAILDRQLDKRNFRRKIELLGILTPLKEWQREGASRPARLYEFSARRFE
                     KLKDKGIIFPF"
     gene            complement(8841..9767)
                     /locus_tag="VFB89_10655"
     CDS             complement(8841..9767)
                     /locus_tag="VFB89_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27809.1"
                     /translation="MYERLVVVTRKTRLEELVERFNTRGQAHFYLEHAGGDFADYDEE
                     HGVYRRSLDQVRHAVSLGLPCQFLDRSLVPTYTFDRSEVVVVLGQDGLVANVAKYAGG
                     QPIIGINPDPSRFDGVLLPFGASAARRVVAETLSGRAHLRSITLAEATLADGQRLLAF
                     NDLFIGARTHVSARYRIRMGSKSEAQSSSGIIVATGAGSTGWMSSIFNMAAGVTAFAG
                     GSRGGSVRLDWEDRRLIYAVREPFVSRHSAAGIVAGLIGPEDHLELESLMPSGGVIFS
                     DGIEADFLEFNSGALGRIRASAQRAHLVVPSD"
     gene            complement(9790..>10466)
                     /locus_tag="VFB89_10660"
     CDS             complement(9790..>10466)
                     /locus_tag="VFB89_10660"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZM27810.1"
                     /translation="PEKLPERLLQVAQVLARTAVQRLPLRDALVRSDPIVAEVLAGLR
                     TSDPVRALGVEILGFNLLAIRPTPEIARALEAEAREALQGRSDEAIYARRNAAVEQER
                     RIKESELETEVAVQAKQRQIREAQIAADIAVEEQRSALLERRVENDRRDADARAYALN
                     ATLEPLRGADWRTLMAVGAGSADPKLMIALAFRELAENAAKIGELNVTPDLLRSLLTP
                     TPQAGR"
BASE COUNT         1811 a         3275 c         3356 g         2024 t
ORIGIN      
        1 ccgctccacc cgcgaaagct gctggctgcg gccggatttg gggtgcgtgc tctgcaatcg
       61 ctcactggct tggcaagccg cttcaagaca aaggcagcac gggctcttct tgccggctgt
      121 ggcgcccact cgatgattgg cctgaccgaa tacctctcgg gtgctttcgg gctgattttt
      181 ttggcaagcg gtcattcggt gggctggcct attcctgcag gcggctccca acggatcgcc
      241 gacgcgttgg ctgcgcactt ggagtcatta gggggaacca tcgtactcgg ccaaaggatc
      301 acttcgatcg accatctcga accggcccgc ggggtgctct tcgatatcag cccgaggcaa
      361 ttgctcgaga tagctggtgg gaggctgagt tgttggcggg ggagtggcct ctacctccgt
      421 caattgcgtc ggttccggca gggccccggg gtgttcaagc tggatctggc actcgacggg
      481 ccgattccgt ggcgggaccc agaggtgctt cgtgcggcga cggtgcatct gggaggaacc
      541 ctcgaagaaa tcgtggcgtc ggaagcggcg gcaaccaacg gacggattcc ggagtccccg
      601 tttgttctac tggcccagtg ttcactgttc gactcgtccc gcgcgcccgc cggccgccac
      661 accgtctggg cctattgcca tgtgcccaat ggcgcgacgg tggacatgac ggagcggatc
      721 ctgacccaga tcgagcgctt cgcgccgggc ttccgcgacc ggattctcgc gacctcgaag
      781 ctcacgccgg ctgatctcga gcgctacaat ccgaacaacc ttggcggcga catcggcggc
      841 ggccggtcgg acatcgggca gtttttcact cgccccgctt ggcggctcga tccttacagc
      901 acgccggatc cgggaatttt tctctgctcc gcttcgacgc ctccaggagc cggcgtccac
      961 ggcctctgcg gctggtatgc cgcacgatcg gcgctgcgag ggattctgcg aggagggcac
     1021 ggtactcaga tagcgtgatg tccgatggtg gattggttct ttgccccgga ctaccggatt
     1081 gcccgctggc taatcgagcg cggcatggcg gcgatctacc tgctcgcatt cgcctgcgtg
     1141 ctcgaccagt ttcccgcgct gctgggcgag cgtggcctcc tgcccgtccc gcaattcctc
     1201 aatgggcttc gatttcggga tgagccgacg ctctttcggc tctattactc agaccgattg
     1261 ctgcgcgcag taggctggac cggcgtactt ctcgccgcaa tcctgctgct tggcctgcct
     1321 caagccggac cgctctggct tcccatgttg gtatggctcg ttctgtggct gctctatctc
     1381 tcgatcgtca acgtcggaca acggttctac tctttcggct gggaatcgct gctgctcgag
     1441 gccggctttc tggcgatctt cctgggaaat gcgagcactg cgcctccgtt cgtgatcatg
     1501 ctgctgttcc gatggctgct cttccgggtc gagctcgggg ctggactcat caagctgcgg
     1561 ggagacccct gctggcgtga gctcacgtgc atggatttcc atcacgagac gcagcccatg
     1621 cccgggcccc tgagctggta cttccatcgg ctcccccgca cgcttcaccg gatcgaagtt
     1681 ctcggcaact acgcggcgca actcgtcgct ccattcatgt tgtttctgcc ccaaccggtc
     1741 gcaggcatcg gggcgctgct catgatcggg acgcagtcat acctgatggt cagcggcaac
     1801 tacgcctggc tcaacgcgct cacgttggtc atcgccttcg caggacttcc cgatgcctgg
     1861 ccaagccacc tgtggcccgg gactctgtcg gcgcaggagt tcgcggtggc gccgtattgg
     1921 tacctggtgc ttgtgttgct ggtgggaggg ctggtggtcg tgctgagtta ctggccggtc
     1981 cggaacctta ttgggccagg tcagcgaatg aactacagct tcaaccgact gcatctggtc
     2041 aacacctacg gagcctttgg aagcgtgacg cgggagcgcc acgaagtcgt cctggaagga
     2101 accacttccg agaaggcgtt cgacagcgcc gtgtggcagg agtacgagtt ccgggccaag
     2161 ccgggggatc cgcggcgacg accaccccag tttgcgccgt accatctccg gctcgactgg
     2221 ctcatgtggt tcgccgcgtt gtcaccgcga tatgctgaag attggttgct cccgctggtc
     2281 catcggctgc ttcagaacga tccggccacg cttcggcttc tcaggcggag cccatttacc
     2341 caggcgccgc cggtctggat tcggggtcag ctgtaccact atcggttcac gaccgggaag
     2401 gagcgacagg cgaccggcgc gtggtgggtc cgcgagctgg tggctgacta tttccctccc
     2461 gtgagactgc cgccgcgtgg ctcgacaagg cgcgacactg ggcagtgacc tagccgagcg
     2521 agaccgggtt gggcacattc cgtcacccat gcggaaattg atcgtgctgc tcgcgctgac
     2581 cgggatcctg gccagctgca ggcaggaccc tcgaccgatc cagctcggcc tggtccttac
     2641 cttggccgac tccggggtcg cgccgatgct tcggggcgcc cgcctggccg tttccgagat
     2701 caaccgggcc ggcggcgtgc ggggacgtcc gctgtctctc gacgttcggg acgacttcgg
     2761 cgatccggac tcggccgtcc gggtcgcggg cgatctttat cgcagcgacg tggtcgcggt
     2821 catcggcagc gcgtattccg ctccgactct cgccgcagcg ccggtgtaca acggcggtcg
     2881 gcgaccgctg attcagatct cgccttccgc atcggtgccg gacttgagca atgccggcga
     2941 ctataccttc cggacctgcc ccaccgacct cgagtacgga gcggcgctgg cccgttgggc
     3001 tcacgatcgc ctggggctga gccgcgcggc catcctctac gtcaacgatg actacggccg
     3061 cggctttcgc ctgaacttcg tccgggagtt cgagcgactg ggcggcgtcg cgaccgtggt
     3121 agctccgttt ctggccagcg atcccgacat caatgcatac ctgatccgct tgcaggcccg
     3181 acacgacgcc gacgtgctag tgcttggtgc aaatctcacg gaaggcctgg tggcactgca
     3241 gcagattcgc gcttccggct tgaagctgcc ggtgcttgcg ggcgacggtt ttgtcggggt
     3301 ggagacccga ggtgatgttg ccgaaggctt gtacgtgtcg accgcatatc tggtagggtc
     3361 gaaacggatg gccaatcgcc gcttcgtaaa tgcctaccgt cacgaatatc cctcggcgcc
     3421 accaccggat cagggcgctg cctcgaccta tgacgtggtc cggctgctcg ccaaatccat
     3481 ccagcaagcc ggtgccgacc gggaccgcat ccgttcggtc attgccggca ccggtacccg
     3541 cacgccccca ttcgacgctg cgatcggacc gatctcattc gattcccacg gggatgtgcc
     3601 gtcgttgggg gcgcgcatcg gtgtcgtgcg acacggggag ctcgagcccg ccgaatagcc
     3661 cgggaaccgt tttctgaaca cgcaggtttg cccttccggc tgttcgattt tcctaccacg
     3721 gaggctcgca tggaacagcg ccgacttggc gcacagggat tggtcgtctc tgcgatggga
     3781 ctcggctgca tgggtatgtc cgagttttat ggcagtcggg atgacgacga atccgttgcc
     3841 acaattcatc gggctctcga gctcggtatc acctttctcg acaccgcgga cgtctacggc
     3901 ccctatacca acgaagagct ggtaggtaga gcaattcgcg accgccgcga taaggtcgtg
     3961 ctcgccacca agtttggaaa cattcgcggc gcagatggga gctttgccgg tattaacgga
     4021 aggccggagt acgtccgtca agcctgcgac gggtcgctca agcggctggg cgtagatcac
     4081 atcgacctgt actaccagca tcgggttgat tccaacgttc caatcgaaga gaccgtcgga
     4141 gcaatggcgg agctggtgcg ggccggcaag gtacgttatc tcggtctctc ggaagccggt
     4201 cctgccacaa tccggcgagc tcacgccgtg catccaatct cggcacttca gacggagtat
     4261 tcgctctgga gcagggatcc cgaggacgat gtcattccgg cttgccggga gctggacatc
     4321 ggcttcgtgc cgtacagccc gctaggacgg ggatttctca ccgggcggtt ccggtcgctg
     4381 gaagatctgc cggccgatga ctggaggcga aacaatccgc gcttccaggg cgaaaacttc
     4441 cagaagaatc tgaatctggt aactcgcatc gaagatctgg cccgctcgaa aggggcgacg
     4501 ccgtcgcagc tagcgctggc atgggtgttg gggcagggtc gcgacatcgt cccgatccca
     4561 gggaccaagc gggtgcgata cctggaggag gacgtggcgg ccgttcacgt ccggctgacg
     4621 gccgacgatc tggcagctat cgacgcggca gcgccggccg gcgtcgcggc gggtgagcgg
     4681 tatcctgccg ccggaatggc gagagtaaat ctctagcagt ggcggagcac atatgaagat
     4741 cgggatcatc ggtgcgggcc ggatcggcgg taccgctgcg cgtctctttg ccggggcggg
     4801 ccacgaggtg gtattgagca actcgcgggg ccccgcgtcg ctttcggatc tcgttgccgc
     4861 tatcaaccgt gaggtggggg aagggcgagc tcaggccgcg acggctgccg acgccgcaaa
     4921 attcagcgac atcgtaatgc tcgctgctcc ctggcgagcc gatgaggctt tgcccgatcc
     4981 cgatctcgtt cgagggaaaa tcgtgatcga cgctatgaat gcctatggcg aatccgggag
     5041 cgtcatcgat ctcggcggat ccggctccag cgaagaggtt gccaagcggc tgcctggcgc
     5101 ccgcgtggtc aaggctttca atacgatcta ttaccagcac ctcgtagggc agggccgtcc
     5161 gaatgcgccc ttggccgagc gtcgtgccat cttcattgcc ggcgacgatc cgacggccaa
     5221 gcgaatcgtg tcagacctca tcaggcagat cggattcgga ccggtggaca ccggcacttt
     5281 gcgtgagggc gggcggaccc agcaaccagg gacaccggtt tacaatcgcg atctcactca
     5341 ggccgaagcc gtcgctatgc tggccggggc atcctgagcg ggagcgcgta tggaatacgc
     5401 gcagctcggc cgatcggggc tctcggtatc ccggatttgc ctcggctgca tgagctatgg
     5461 cagccctacg tggcggccct gggtgctcga cgaagccacg gcgcggcctt tttttcgccg
     5521 agccattgaa gccggaatca atttcttcga caccgccgac atgtactcgg tcggtgcgag
     5581 cgaagaggtg accggcaggg ccctacgaga gctggcccgg cgtgaagaga tcgttctcgc
     5641 cacgaaggtg ttttttccgc tcaagcctgg cccgaatcag ggcgggcttt cccgcaagca
     5701 tatcatggag gcctgcgagg cgagtctcag gcgccttggc ttcgagacga tcgatctcta
     5761 ccagatccat cggttcgatc cggtcacgcc gctggaggag acgctggccg ccctggatca
     5821 tctggtgcat tcgggcaagg tccgctacat cggagccagc tcgggatacg catgggagat
     5881 ggccctggcg ctgtcgatct cggagcggaa cgggtgggcg cgattcatca cgatgcaaaa
     5941 ccactacaac ctcatttacc gcgaggagga gcgtgagatg ctgccgctct gccggcacga
     6001 gggtgtcggc gtgattccct ggagtccgct cgcgcgtggc ctgctggccc gtccaaggcc
     6061 agccgacaac cgactcaaga gcggggggac ccatcgcgcc gagagcgacg cctactcgcc
     6121 tcggctctac gacagcgccg gcgattggga tgtagtcgac gcggtggaaa gcgtcgccaa
     6181 gaagcgaggc acgagcatgg ctgaggtggc gctggcctgg ctgctcagcc ggcccggggt
     6241 cactgcgcca atagtgggtg ccaccaggcc ggagcacctc gaagctgcgc tccgtgctgt
     6301 ctacctcgag cttagggacg atgagcgggc ggcgctggaa gctccctacc gaccccacgc
     6361 agtgcgcggg atcgagtaat cactcgacgc acaatcagta acgccagctt gtcgtgtccg
     6421 cgtcaggcgg atgattcttc cggatctgat ccatgacccg aggaatgaac atctggtgat
     6481 accggagcct cgagatggcg ttgggttggg tgtggtcgcc gttccagcag tgctccgcgc
     6541 ggactccgta gtccacctcg gcgtccgcgg gaggatcctt gagggatttc aggacctctt
     6601 ccgtcagata aaccgcgttg ttcagatagt agttgtcagc ctcgcctaca tagatgtgaa
     6661 gtttcccgcg cactttgggg ccaagggtct tccaatcccg ttggaggatg ttggaaaggt
     6721 cgtagtgttc ccgccaatac tccgcgactg accgatcgat ttcgcccgtc agcttgttcc
     6781 agatcggctt ggggtatcca tccttgtcgg tcggcgaata gacggcctgc cagatatccc
     6841 actgctctcc cgaacggccc cgagttgcca gggccagctc tttatggttc agctccgcga
     6901 gagtcgccgt gacgcggccc agccaatcgc gctctccggg cctaggggtg cgtttccagc
     6961 ggctatccaa gcgataggcg ttggtgtctc gatagatgtt cacgacggtg tacgctctga
     7021 aatcgatggg atcggggcag gcgacccagg ctccattgaa ctcgtcgggg taaaacacct
     7081 gcgccgccat cgcctcccag ccgccggtgg agcctccgta ggtgaagcga gcccatccct
     7141 ttccgatccc gcgatacttc tgctcgatgt aaggaatcag ctccttcatg atcgcgtcgc
     7201 cgtagggtcc gttgttggcc gagttcaccg cgtacgaatc gtcgtagtag ggagtagggt
     7261 gctggatctc aaccacgatg acgcggggaa agcccggtcc ggtccagtcc ttgaaaagct
     7321 gataggcgta ctgttcctgg atccgattgt atcccttgag tcggaagcgg gtgctcgaat
     7381 caggcgccag gttcgggtct ggtggcgtct cgcgccaacc ctcgatcgat tccggaaaat
     7441 gcccgtgcga caggatgagg ggataccgtg cctctggatg gtcgtagaac ccttcgggca
     7501 gaagaatgtg tgcaccgagg tagaccgggt ggccccagaa gcgggtgagc aagtcgctct
     7561 gaatgcggat gtgtttgacg tacctggtct cggccggcgc tggaatgggt ggaatgattt
     7621 ggtcgagcac cacgggaaac ggcccaatct tgagacgagg gtcgatctga atccatcgag
     7681 gcacactgta cagattgccc ggcttcttct cccagtgctg cccctctccc atgtcggggg
     7741 gaagcttcac gacgtggcca tccgcgcgat gaaaggtctc gtaccgattg agcaccgctt
     7801 gaacccagta gcggcccggc ggaatcgctc gcaacctctg tagcgggtat ccgaacgcgg
     7861 ctggaccgac gacggccggc ttgcccggcg ccagtcgatc gacatttacg ccgaagacga
     7921 gttgagtctc cggtccatct gcgatctgga agcgaggctc tttggatgaa tcggtcgaga
     7981 ccagcaacaa tacgcggcca tcgagagcct cggcgcttcg cgcccgagga aagctcaccg
     8041 caaagctgga acccgcacgg gggacctttg gctgctgggc ggcgagcagt ggtgtcaggg
     8101 aagcgagaca gacgagccag gcaggccaga caaagcgacg catgacggcc ctcagaatgg
     8161 gaaaatgatg cctttgtcct tgagcttttc gaaacgtcga gctgagaact catacagccg
     8221 ggccggtcgg ctggcgccct cccgctgcca ttccttgaga ggtgtcagga tccccagcag
     8281 ctcgattttc ctgcgaaagt tgcgcttatc gagctgccgg tcgaggatcg cctcgtagac
     8341 ccgctgaagc tgcgtgagtg tgaattgtcg gggtaacaat tggaatccca ccgtggtgta
     8401 ctcgagcttg ttgcgcagcc gctcaatagc gtacttgagc attgcgtcat gatcgaaagc
     8461 aaggggcgga agctcggctg tggaccacca ctgcgccgct gccgcgtcgc tgcctcccac
     8521 cggtgcggcc cgatcagccg caatcagcgc atagtatgcg acggtaatca cgcggcccct
     8581 cggatctcgg cccggctctc cgaaagtgta gagctgctcg aggtacacgt cccgtacccc
     8641 ggtctcttcc tccagctcgc gcaacgccgc agtctctaac gattcggctt cccgaacgaa
     8701 cccccccgga atggcccagc ggccttcgaa cggcggcacg ccccggcgaa ccagcaagac
     8761 gttgagccgg tcgaaccgga tggtgaagat cacgatgtcg acggtgacgt gaacccgctc
     8821 cggcggcagg ttggacgtca tcagtcagat ggtacgacga ggtgggcgcg ctgggctgac
     8881 gccctgattc ggccgagggc gcccgagttg aactcgagaa aatccgcttc gattccgtcg
     8941 ctgaaaatca cgccgccgct tggcatcagc gactcgagct caagatgatc ctccggtccg
     9001 ataagacccg cgacgatgcc agcggcagag tgccggctga cgaatggctc gcgcaccgca
     9061 tagatgaggc gtcgatcttc ccagtccagc cgaacgctgc ctccgcgcga gccaccagca
     9121 aacgctgtca ctccggccgc catgttgaat atcgaggaca tccagccggt cgatccggcc
     9181 cccgtcgcga cgataatccc gctggaggac tgagcctcac tcttgctgcc catccgtatg
     9241 cgatagcggg cggacacatg ggtccgcgct ccgataaaga gatcgttgaa tgcgagcagg
     9301 cgctgtccgt cggccagcgt cgcttcggcc agcgttatgg agcgcaggtg cgcccgccca
     9361 ctgagcgtct cggcgacgac gcgccgcgca gcggaggcac cgaacggcag cagaacaccg
     9421 tcaaagcgag atggatcggg gttaatgcca atgatgggct ggccgcccgc atacttggcg
     9481 acattggcca cgagcccatc ctggcccagc accacgacca cttcggatcg gtcgaaagtg
     9541 taggtgggta ccagtgagcg gtccaggaat tggcagggca atccgagaga gacggcgtgc
     9601 cggacctgat ccagcgagcg tcggtagacc ccgtgctcct cgtcatagtc ggcgaaatcg
     9661 cccccggcgt gctcgagata gaagtgagct tgccctcgcg tgttgaaccg ctcgaccagc
     9721 tcttcgagcc gggtcttgcg agtcaccact accaatcgtt cgtacatgac gagagtcgtg
     9781 ctgccgcgct cagcgccccg cctggggcgt gggcgtgagt agcgaccgaa gcaggtcggg
     9841 cgtcacgttc agctcaccga tcttggccgc attttcagcc agctcccgaa aggccagcgc
     9901 gatcatgagc ttgggatcag cgctaccggc gccgacggcc atcagcgtgc gccaatcggc
     9961 gccgcggagc ggctcgagcg tggcgttgag cgcgtacgcg cgcgcatcgg cgtcccgccg
    10021 atcgttctcg actcgccgct cgagcaacgc cgagcgttgc tcttcgaccg caatgtcggc
    10081 cgcgatctga gcctcgcgaa tctgacgctg cttagcctgc accgcgacct ccgtctccaa
    10141 ctcactctcc ttgatgcgac gttcctgctc gacggctgcg ttgcgccggg cgtagatggc
    10201 ctcgtcggaa cggccctgca acgcttcgcg ggcctccgct tcgagtgcgc gggcaatctc
    10261 gggtgtgggg cggatcgcca gcaggttgaa gccaagaatc tcgacgccaa gcgcgcgcac
    10321 cggatcgctc gtacgcagcc ccgccaacac ctcggcgaca atcggatcag acctcaccag
    10381 tgcgtcgcgc agcggcagcc gctgtactgc cgttctcgcg agcacctgtg ccacctggag
    10441 cagtcgctcc ggcagcttct cgggat
//
LOCUS       DASRJQ010000469         3592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_4012832, whole genome shotgun sequence.
ACCESSION   DASRJQ010000469 DASRJQ010000000
VERSION     DASRJQ010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4012832"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VFB89_10665"
     CDS             <1..504
                     /locus_tag="VFB89_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27811.1"
                     /translation="PRSRMSTSRILAVTAAVALAGALTANAQQVSEKANRNPKLITTE
                     EVEAARVNNAYQVIEKLHPEWLRRTARRTTLDGGRPRPPSNSGSSDADGASPASSDAN
                     YVQANEPPTTSAVFVDGTEMGGAEELTQVQSNLILDIRYLTGSDAETKYGPRFSNGVI
                     EVRLKTR"
     gene            543..2111
                     /locus_tag="VFB89_10670"
     CDS             543..2111
                     /locus_tag="VFB89_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27812.1"
                     /translation="MRYRWGALLAGAVWLPGAAAAQFSPVGVPKGLLRVELDGALETV
                     DRRYFGGTSEEFAADLASPALGADRIPGFAEADAALGRITQVGGARLNLGQLTATAQG
                     SRNSAAVGLGFGITSHISVFARLPLARTTVKTTLTLDSTSSNAGINPADPVFGAQAGA
                     AQTSQFISDFTGALQSLEARLQAGVYDGDPAVRALAQSTLNSGTALRNDLAGLLLDPA
                     TASTFVPTAGSQLGTALNARIQALQATLSGSLGVTGFTTTPALPVTRASQDALTAYLT
                     DPRGPIAGRVAEAPLSQRGDAEVGAVFTLIDRWNDDAKGTGIRFAVSGLYRLGTGFPA
                     RPSFFLDIGTGTGHAALGGTATADIGVGRFGIRLTGGYLQQRPADDLLRVTPPDQPYA
                     PFNHIKTVRVNPGDVFTLGAHPFVRLVPGFAIQAGVDHLHEGAETVAYLSASDSIPGV
                     PASVLALDSEIGVTTFSAGITYATPGISKPGAKGIPVDAFWTLGRVVAGSGGRVSKFQ
                     SMRGGVRVYFRIFQ"
     gene            2182..>3592
                     /locus_tag="VFB89_10675"
     CDS             2182..>3592
                     /locus_tag="VFB89_10675"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HZM27813.1"
                     /translation="MLIPPPDLFAQNTGTTRLRDLSQQFWTWRAANQPASGDDIPRIE
                     RPKDWTPDWSAEAVAARRKALAAFEGEWRSVDTTGWSRADQVDYRLMGSALARVHWEL
                     DVTRGWRRNPLFYLDQTVGVLHQDLLRPPPFDSVRTSELVHRLERIPRTLEEARANLT
                     ESLAPFTRLAVTSLDGMGPRLERVGKALGPLLEGPEASRLATVLTSASAALEQYRRWL
                     EQRMAGLSEKTAIGRDAYLGFLRSVALVPYTPEQLVRMAQQDWARSVAFEAYEQQRND
                     SLPKLESFPTLPAQLAQEKEDELAIRRYLAAKDLISVPATLRHYRTAPLPPYLEPLSD
                     WGVNDDLTSATRLSEDGVSYRPPPAPDLGYFYRASAKDPRPLMVHEGVPGHYMQLALA
                     WAHEDPIRRHYYDSNSNEGIGFYAEEMMLQAGLFDDRPRTREIIYNFMRLRALRVEVD
                     VKLATGEFTIPQAADYLETS"
BASE COUNT          628 a         1197 c         1129 g          638 t
ORIGIN      
        1 ccgaggtctc gaatgtcaac gtcgcgcatc ctcgccgtga ccgccgccgt ggcgctcgcg
       61 ggcgcgctca cagccaacgc ccaacaggta tcggagaaag ccaaccggaa tcccaagctc
      121 atcaccacgg aagaagtgga agcggctcgc gtcaacaatg cctaccaggt catcgagaag
      181 ctgcaccccg agtggcttcg ccggaccgct cggcgtacaa ccctggacgg cggtcggccc
      241 cggcctccga gcaacagcgg ctccagcgac gccgacggcg cgtcgccggc gtcgtccgac
      301 gccaactacg tccaggccaa cgagcccccg accacgtcgg cggtcttcgt ggacggcact
      361 gagatgggag gagccgagga gctgacgcag gtccagtcta atctgattct ggacatacgc
      421 tatctgaccg gcagcgacgc tgagaccaag tacgggcccc gcttttcgaa tggggtaatc
      481 gaggtgaggc tcaagacccg ttgaccgacg gcctctacag cttccgtccc gttcggccct
      541 gagtgaggta ccgatggggc gccctgctcg cgggtgccgt gtggctgccc ggagcagccg
      601 cggcgcaatt ctctcccgtc ggcgttccca agggcctgct tcgggtcgag ctggatgggg
      661 cgctcgaaac ggtggatcgt cgctacttcg gcggaacatc ggaggagttt gcggccgacc
      721 tcgcgagtcc ggcgctcggc gctgaccgca tccccggttt cgcggaggcc gatgccgcgc
      781 tcggacgaat cacccaagtt ggcggtgctc gcctgaatct cggtcagctg acagcgacgg
      841 cccaaggctc ccgcaattcg gcggcagtcg gcttgggctt cggtatcacc tcgcacatca
      901 gcgtctttgc ccgtcttcca ctcgcccgga ccaccgtcaa aacgaccctg acgctggaca
      961 gcacctcgag caatgccggc atcaatccgg cggatccggt gtttggggct caggccggcg
     1021 ccgcgcagac ctctcagttc atctccgatt ttaccggcgc actgcaatcg ctcgaggccc
     1081 gacttcaggc cggcgtgtac gacggtgacc cagccgtccg cgcgctggcc caaagcacgc
     1141 tcaactccgg cacggcactc cgaaatgacc tcgccggtct gctgctcgat cctgccacgg
     1201 cctcgacgtt tgtcccgacc gccggaagcc agttggggac cgcgctgaac gcgcggattc
     1261 aagcgttgca ggccactctc tccggcagcc taggcgtcac tggtttcacg actacaccgg
     1321 ctctgcccgt gactcgagcc agccaggacg ctctcaccgc ctacctgacc gatccgcgtg
     1381 gaccgatcgc cgggcgggtt gccgaagcac cgctttccca acgaggcgat gcggaggtcg
     1441 gcgccgtgtt tactctgatt gaccgctgga acgatgatgc gaagggaacc ggcattcgat
     1501 tcgccgtcag cggcctatat cgcctgggaa ccggctttcc ggcgcgtccc agctttttcc
     1561 tcgacatcgg caccggtacc ggccatgcgg cgttgggggg aaccgcaacg gcggacatcg
     1621 gggttggtcg cttcggaatt cggctgaccg gtggctatct ccagcagaga cctgcggacg
     1681 acttgctgcg ggtgacgcca ccggaccagc cctatgcgcc attcaaccac atcaagacgg
     1741 tgcgggtgaa tccgggtgat gtgttcacgc tgggcgctca tcccttcgtc cgcctcgtcc
     1801 ccgggtttgc catccaggca ggcgtagatc atctgcatga gggtgccgag acagtcgcat
     1861 acctgagcgc ttcggattca attcccgggg ttcctgcatc ggtcttggca ctcgactcgg
     1921 aaattggagt gacgaccttc tccgccggca tcacctacgc gacgcccggc atcagcaagc
     1981 cgggcgcgaa aggcatcccg gtggacgcct tctggactct cgggcgggtc gtggcgggga
     2041 gcggaggacg agtttccaag ttccagtcca tgcggggagg cgttcgggtg tacttcagaa
     2101 ttttccaata ggaggcggac cctgacaccc cagcgacgcc agccactgtg cacatggggc
     2161 gcactcgcag tcttcaccgc ggtgctgatc cctccgcctg atctgttcgc gcagaacacc
     2221 ggtacaacgc gtcttcgcga tctttcgcaa cagttctgga cctggcgcgc cgcgaaccaa
     2281 cccgcttccg gcgacgacat tccacgcatc gagcgaccca aagactggac gcccgattgg
     2341 tcggccgaag ccgtggcggc ccggcggaaa gcattggccg cgttcgaggg ggagtggcgg
     2401 tcagtcgaca cgacggggtg gagtcgtgcc gaccaggtgg attatcgcct catgggttcc
     2461 gcattggccc gggtccattg ggagctggac gtcacgcgtg ggtggcggcg caatccgctg
     2521 ttctatctcg atcaaaccgt tggtgtcctg catcaggacc tgcttcgacc gccgccgttc
     2581 gactcggtgc ggaccagcga gctggtccac cggctggaac ggatcccgcg tacgctggaa
     2641 gaggcgcgcg ccaacctcac cgagagtctt gccccattca cgcggctcgc cgttacgtcg
     2701 ctggatggaa tggggccccg gctggaaagg gtgggcaagg cactgggacc attgctcgag
     2761 gggccggagg ccagccggtt ggccactgtc ctgacctccg ctagtgcagc gctcgaacag
     2821 tatcgcaggt ggctggagca gcggatggca ggactgtctg agaaaaccgc gatcggtcga
     2881 gacgcgtacc tgggctttct ccgcagcgtc gcgctggtcc cctacacgcc ggaacagtta
     2941 gtccggatgg cgcaacagga ctgggcccgt tccgtcgcct tcgaggcata cgagcagcag
     3001 cgcaacgaca gccttccgaa gcttgagtcg tttccgacgc tcccggctca actggcgcaa
     3061 gagaaggagg acgagctggc aatccgtcgc tatctcgcgg caaaggacct aatcagcgtt
     3121 cccgctacgc tcaggcacta tcgcactgct ccgcttcctc cctatctcga gccgttgtcc
     3181 gattggggcg tgaatgatga cctcacctcc gcaacccgac tctcggagga cggggtgagc
     3241 tatcgtccgc cccccgcgcc cgatctaggc tatttctatc gcgcgagcgc caaggatccg
     3301 cgtcccttga tggtgcacga gggcgtcccc gggcactaca tgcagctcgc gctagcctgg
     3361 gctcatgagg atcccattcg acggcattac tacgactcga actcgaacga agggatcggc
     3421 ttctacgctg aggagatgat gttgcaggcc gggctcttcg atgaccgccc ccgcactcgc
     3481 gagatcatct acaacttcat gcgactccgc gcacttcggg tagaagtgga cgtgaagctc
     3541 gcgacgggag agttcaccat tccccaggcg gccgactacc tcgagaccag cg
//
LOCUS       DASRJQ010000470         6246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2383569, whole genome shotgun sequence.
ACCESSION   DASRJQ010000470 DASRJQ010000000
VERSION     DASRJQ010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6246
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2383569"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="VFB89_10680"
     CDS             <1..1030
                     /locus_tag="VFB89_10680"
                     /inference="COORDINATES: protein motif:HMM:NF013442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27814.1"
                     /translation="FDFGLYFFHNAAELLRRGSGPYFYLPKLESHLEARLWNQIFEFA
                     QDTLGIPRESIRATVLVETILAAFEMDEILYELRDHAAGLNAGRWDYLFSVIKNFRDR
                     PEFVLPDRSQLTMTVPFMRAYTELLVSTCHRHGAHAIGGMAAFIPNRKDPEVNASALA
                     RVREDKGREAADGYDGAWVAHPDLVPVVAEVFEDRLGGQPHQKERQRTDVRVGQAELL
                     DVRVPGGRITEAGVDNDISVGLQYLARWLEGSGAVAINNLMEDTATAEIARAQLWQWI
                     RHSVRTETGEPVTVERCRARLADTLARLEAEGTPGRLRDAATLLDQLFSAAEFPEFLT
                     LAAYAYLK"
     gene            1078..2448
                     /locus_tag="VFB89_10685"
     CDS             1078..2448
                     /locus_tag="VFB89_10685"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZM27815.1"
                     /translation="MTGLCPPPGPTARFPGQLLLAFRRDPLGLLQRMARDYGDVSLVR
                     LGNRRVHLLNHPDLIKDLLVTEQRRFHKGRGLELAKHLLGEGLLTSEDEFHRRQRRLM
                     QPAFHSQRIASYASAMVQHAERTSERWSRLVAQTPTPPVVDIHQEMMRLTLGIVGKTL
                     FNTEVEDQAREIGQAIATSMELFRSFTTLPFAPLLERLPLPSTWRFLKARERLDATIY
                     GLIKDRRSTGEDRGDLLSMLLMAQDDEGSGGMSDRQVRDEAMTLFLAGHETTSNALTW
                     TWYLLSQHPAVETRLHAEIDQVLGGRSAVAEDMHRLVYTWQVLAESMRLYPPAWVIGR
                     RAIEDYEVAGYRIPRDSIVLASQWVTQRDARFFPEPDRFDPDRWTEVARAARPRFAYF
                     PFGGGARVCIGEQFAWLEGVLVLATLARRWRPRLAPGHAVALQPTITLRPRGGMKMDL
                     YGVGNS"
     gene            complement(2610..2954)
                     /locus_tag="VFB89_10690"
     CDS             complement(2610..2954)
                     /locus_tag="VFB89_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27816.1"
                     /translation="MPLVRAAFRAAADLLLALRRRAAERACLERARRDAALRGSRLSA
                     RSMPRDRLRDGFFLRLLWPLRYALSALLRVLLDVVPFFGGRSFTPARRAFDKPIAIAC
                     LVERAPCLPSRM"
     gene            3034..4824
                     /locus_tag="VFB89_10695"
     CDS             3034..4824
                     /locus_tag="VFB89_10695"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HZM27817.1"
                     /translation="MPLPIEDYGLIGDCETAALVGRDGSIDWLCVPRFDSPACFAALL
                     GTPEHGRWLIAPAAPIRQVRRRYRPGTLVLETEFETDAGVVAIIDCMPVRTETPHVVR
                     FVEGRRGNVPMRFELVIRFDFGSLIPWVRSRDDGIVAIGGANGLRLRTPVPLEGKDFT
                     TVAEFSVGPGECVPFTLTCFPSHKPEPAAPDADVAVRETEAWWGEWSGRCGYKGKWAE
                     PVVRSLITLKALTYAPTGGLVAAVTTSLPEQLGSNRNWDYRYCWLRDATFALYALMVG
                     GYKEEAEAWRTWLVRAVAGRPDHAQMMYGVKGERLLPESELPWLPGYKGSAPVRVGNA
                     ASKQFQLDVYGEVCDSLYCAARAGIPPDENAWQVKRAMIEFVASVWKDPDEGIWEVRG
                     PRRPFTHSKMMAWVAVDRGVKAVERFGQEGPVERWRHLRAAIHADVLEHGFDPGLGAF
                     VQYYGSKLLDASLLMMPLVGFLPADDPRVLGTVSAIQKQLMSDGLVARYHTVPDVDGL
                     PPGEGAFLPCTFWLADNLALQGRVDEAREIFERLLSLRNDVGLLAEEYDPVSRRMLGN
                     FPQAFTHVGLINTAWNLDRDVGPAVQRHRD"
     gene            complement(4833..5735)
                     /locus_tag="VFB89_10700"
     CDS             complement(4833..5735)
                     /locus_tag="VFB89_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27818.1"
                     /translation="MRVPVLGLLSPAVVAAMMAAAPPAHSQTIALPLRGEGEFTLPDK
                     QIDVRSGSVLLNADSSVKVFFIDAGTLLTAFVGEWRRADTRTVEFAVLDVPGQRNVKG
                     SGRIHFRTDGAIDALDVRGQADGREFSARFDNGMTTAAVSTSELRVSRQDPSPRQGSR
                     AAQAPRQDSEEWPWGGSLYLVDVTRRGEGVLQNSEGAEYHFDRARLVLGENDEFQLTI
                     VGDTKMVLAGTWSGDLRFGPVPLELREAFGGKVEGMGRVWIRDRSWDRDFTLERIELD
                     GWSNGHRFTMYFEAKTPPGDAGSQ"
     gene            5783..>6246
                     /locus_tag="VFB89_10705"
     CDS             5783..>6246
                     /locus_tag="VFB89_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27819.1"
                     /translation="MPGSLSRVVLRPQLHWLIALLPGAAPSGAVAQVPRDSSATVALT
                     TGISQTLARHRAATISDVRYDLTLDVSPLDSAIGCITIRFQKSDSGDAIIDFRGRRLT
                     RAVANGRPIPAGTAANGHILIPARLLRVGQNQLDFAFVADIAPSGASIIRSH"
BASE COUNT         1110 a         2080 c         2007 g         1049 t
ORIGIN      
        1 cttcgacttc ggactgtact tcttccacaa cgccgccgag ctgctgcgcc ggggcagtgg
       61 gccctatttc tacctgccca agctcgagag ccacctcgag gcccggctct ggaaccagat
      121 cttcgagttc gcgcaggaca cactggggat tcctcgcgag tccattcgcg ccaccgttct
      181 ggtggagacg attctcgcgg cattcgaaat ggatgagatc ctctacgagc tgcgcgatca
      241 tgcggccggc ctcaacgccg gccgttggga ctacctgttc agcgttatca agaacttccg
      301 ggatcgcccc gagttcgtgc tcccggaccg gtctcagctc accatgacgg ttccgttcat
      361 gcgggcgtac acagagctgc tggtctcgac ttgtcaccgg cacggcgctc atgctatcgg
      421 cggcatggcg gcgttcatac ccaatcgaaa ggatcccgag gtgaacgcgt cggccctcgc
      481 gcgggtgcgc gaagacaagg gacgcgaagc ggcggatggc tacgacggcg cctgggtggc
      541 acatccggac ctggtaccgg tggtggccga agtcttcgag gaccgcctcg gtggccagcc
      601 acaccagaag gagcgccagc gaaccgacgt tcgagtaggt caagccgagc tgctcgacgt
      661 acgcgtgccg ggcggaagga ttacagaagc gggcgtcgac aacgacatct cggtcgggct
      721 gcagtacctg gcccgctggc tcgaggggtc gggtgccgtc gccatcaaca acctgatgga
      781 agacacggcg acagccgaga ttgctcgcgc gcaactctgg cagtggatcc gacacagtgt
      841 gcggaccgag acgggtgagc cggtgacggt cgagcgctgt cgcgcccgtt tggccgacac
      901 gctagctcgc ctggaagccg aagggactcc ggggcgtctc cgcgacgcgg cgacgctgct
      961 ggatcagctc ttttcggcgg ccgaatttcc cgagttcctg accctggcag catacgccta
     1021 cctcaagtga acgggctcac caccagcata taattcgaag tcccattcgt ctctcgcgtg
     1081 actggcctct gccctcctcc gggtcccaca gcgcgcttcc ccggtcagct gctccttgcg
     1141 tttcggcgcg acccgctggg gctcctccag cgcatggccc gtgactacgg ggacgtctct
     1201 ctggtccggc tgggtaatcg ccgggttcac ctcctcaacc atccagatct gatcaaggac
     1261 ctgctcgtca ccgagcagcg tcgctttcac aagggtcgcg gcctcgagtt agccaagcat
     1321 ctcctgggcg aggggctgct gaccagtgaa gacgagttcc atcggcggca gcgccggctg
     1381 atgcaacccg ccttccactc gcagcggatt gcctcatacg cgagcgccat ggtgcagcat
     1441 gctgaacgga cctccgagcg ctggtcccgg ctcgtcgccc aaacacccac gcctccggtc
     1501 gtcgacattc accaggagat gatgcgactt acgctcggca tcgtgggcaa gaccctgttc
     1561 aataccgagg tggaggacca ggcccgggag atcgggcagg caatcgcgac ctccatggaa
     1621 ctgttccgta gcttcaccac cctaccgttt gctccgttgc tcgagcgtct gcccctgcct
     1681 tcgacctggc gctttctcaa ggccagagag cgcctcgacg ccacgatcta cgggctgatc
     1741 aaggaccgcc ggagcaccgg cgaggaccgg ggcgatttgc tctccatgct tctgatggct
     1801 caggacgatg agggaagcgg cgggatgagc gatcggcagg ttcgcgacga agcgatgacc
     1861 ctctttctgg ccggacacga gacaacgtca aacgccctta cctggacctg gtacctgctc
     1921 agccagcatc ccgcagtcga aacccgtctc catgcggaga tcgatcaggt tctcggcggc
     1981 cggagcgccg tagctgagga catgcaccgc ctggtctaca cctggcaggt cctggccgag
     2041 tcaatgcggc tttatccgcc ggcgtgggtg atcggccggc gtgcgatcga ggactacgag
     2101 gtcgcgggat accggatacc aagggactcg atcgttctgg caagccagtg ggtcacccaa
     2161 cgcgacgccc gcttttttcc cgaaccggac cgtttcgatc cggaccgttg gaccgaggtg
     2221 gcccgagcgg cgcggccccg attcgcctac tttcccttcg gcggcggcgc gcgcgtctgc
     2281 atcggcgagc agttcgcgtg gctcgagggg gtgctcgtgc tcgcgaccct ggcgcgacgc
     2341 tggcgtcccc gtctcgcccc gggacatgca gtcgcgctcc agccaacgat cacccttcgc
     2401 ccccgaggag gtatgaagat ggacctctat ggggtcggga attcgtgatc catgccacgg
     2461 cccctcatcc atccgttggg cacgatacag ggcaccccac aggaggtacc catgccagag
     2521 aaagagacaa tggagcgtgc ccacgaagac gctcgtgaag gaaaatcgcc ctccacccag
     2581 gcaggcgagt tcgtgcggga ggaaatacat cacatccggg agggtaaaca cggcgcccgc
     2641 tccaccaagc aggcgatcgc gatcggcttg tcgaaggccc ggcgcgccgg cgtgaagctg
     2701 cgtccgccga agaagggcac gacgtcgaga aggacccgca agagtgcgga aagcgcgtac
     2761 cgcaagggcc agagcagccg aagaaagaag ccgtctcgga ggcgatcccg tggcatcgag
     2821 cgggcgctca agcgcgaacc ccgtagtgcc gcgtcgcgcc gagccctctc gagacaggct
     2881 cgctcggcag cacgccgtcg gagcgcgagc agcagatccg cggcggcacg gaaggccgcg
     2941 cggaccaaag gcaagcgcgg tcggtcagct gcagcgaaga aggcagcaag aacgcgggct
     3001 cggcggaagt aaccggcggc cgccccacaa gacgtgccgc ttcccatcga ggattacggc
     3061 cttatcgggg actgcgagac cgcggccctg gtaggccgcg acgggtcgat cgactggctc
     3121 tgcgttcccc gcttcgactc gcccgcctgc ttcgcggccc tgctcggcac gccggagcac
     3181 ggccgctggc tgatcgcgcc cgcagcaccc atcaggcagg tccgacgccg ctacagaccc
     3241 ggcacactcg tgctagagac cgaattcgag actgacgcgg gtgtggtcgc cataatcgac
     3301 tgcatgccgg tgcgaaccga aacgccccac gtggttcgtt tcgtcgaagg tcgacgcgga
     3361 aacgtgccta tgcgattcga gctggtgatc cggttcgact tcggctcact catcccgtgg
     3421 gtgcgcagcc gggacgatgg gatcgtcgcg atcgggggcg ccaatgggct tcgcctgcgc
     3481 actccggtgc cgctcgaagg gaaggacttc accacggtcg ccgagttttc cgtagggccc
     3541 ggtgagtgcg tgccgttcac gctgacctgc tttccctcgc acaagccgga acccgctgca
     3601 cctgacgcgg acgtcgcggt tcgggaaacc gaagcgtggt ggggggagtg gtccggccgc
     3661 tgcggctaca agggaaagtg ggccgaaccc gtcgtccgat ctctgatcac cctcaaagcc
     3721 ctgacctacg cccccaccgg cgggttggtc gcggcggtga cgacctcgct cccggaacag
     3781 ctcggtagca accggaactg ggactaccgc tactgctggc tgcgcgacgc cacttttgcg
     3841 ctctacgccc tcatggttgg tggctataaa gaggaagcgg aggcctggag gacctggctg
     3901 gtgcgtgctg tcgccgggcg ccccgaccat gctcaaatga tgtatggggt aaaaggtgag
     3961 cgcctgctac ccgagtcgga gctgccctgg ctgcccggct acaagggctc ggcaccggtg
     4021 cgtgtcggca atgccgcgag caaacaattc cagctcgacg tgtacggcga agtgtgcgac
     4081 tcgctttact gcgccgcgcg agccggcatc ccgccggacg agaatgcgtg gcaggtcaag
     4141 cgggcaatga tcgaattcgt cgcgtccgtg tggaaggatc ccgacgaagg gatctgggag
     4201 gtacgcgggc cgcggcggcc gttcacccac tcgaagatga tggcgtgggt cgcggtagat
     4261 cggggagtga aggcagtgga gcggttcggg caggaagggc cggtggaacg atggcggcat
     4321 cttcgcgcgg cgattcacgc cgacgtcctc gagcacggct tcgatcccgg gctcggagca
     4381 ttcgtgcagt actacggctc caaactgctg gacgcgagcc tgctcatgat gccgctggtc
     4441 ggttttctcc cggccgacga cccccgggtt ctgggcaccg tgagcgcgat ccagaaacaa
     4501 ctcatgagcg acggtctggt cgcccgttac cacacggtgc cggacgtgga tgggctgccg
     4561 ccgggcgaag gcgcgtttct tccctgcacc ttctggctgg ccgacaacct ggcccttcag
     4621 ggacgcgtcg atgaggcccg cgagatcttc gagcggctgc tctcactccg gaacgacgtc
     4681 gggctcctcg cggaagaata cgaccccgtg tcgcgcagaa tgctcggcaa tttcccgcag
     4741 gccttcaccc acgtcggact catcaacacc gcctggaacc tggaccggga cgtaggaccg
     4801 gccgtgcagc ggcaccggga ctaagccccg gctcattgac tcccagcgtc ccccggaggc
     4861 gtttttgcct cgaagtacat cgtgaaccgg tggccgttgc tccagccgtc gagctcgatg
     4921 cgctcgagcg tgaaatcgcg gtcccacgat cggtcgcgaa tccacacgcg ccccataccc
     4981 tcgacctttc cgccgaacgc ctcgcgcagc tccagtggca ccggcccaaa ccgcaggtcg
     5041 ccgctccagg ttccggccag caccatcttg gtatcgccaa cgatggtcag ctgaaactca
     5101 tcattctcgc cgagcacgag tcgagcgcgg tcgaagtgat actcggcacc ctcggagttc
     5161 tgcagaaccc cctcgcctcg ccgcgttacg tcgaccaggt acaatgagcc gccccacggc
     5221 cattcttcgg agtcctggcg gggggcctgg gcagcccggg aaccttggcg gggggacgga
     5281 tcctggcgcg atacgcgcag ctcggatgtg gagactgccg ccgtggtcat cccgttgtcg
     5341 aaccgcgcag agaactcacg gccgtcggcc tgtccgcgta catcgagcgc gtcgattgcc
     5401 ccgtcggtgc ggaagtgaat gcggccgctc cccttcacgt tgcgctgtcc gggaacatcg
     5461 agcaccgcga actcgacggt gcgggtgtcg gcgcgccgcc actcgccgac gaatgcggta
     5521 agcagggtac cggcatcgat gaagaagacc ttcacgctgc tgtcggcgtt cagcaggacc
     5581 gagccggatc gtacgtcgat ctgcttgtcg ggaagcgtaa actcgccttc gcctcgcagt
     5641 ggcagggcga tggtctgcga atgagcgggt ggagccgcag ccatcatcgc agccaccacc
     5701 gcaggggaaa gcagaccgag aaccgggaca cgcatagaac ctcctggcag taactccacc
     5761 caacgacggt atattcgcct gcgtgcccgg atccctcagt cgagtcgtcc ttcggccgca
     5821 gctacactgg ctgatcgcgc ttcttcccgg cgcggctcca tccggagctg tggcccaggt
     5881 gccgcgcgac agctcggcga cggtggccct cacaaccgga atctcgcaga ccctcgcccg
     5941 tcaccgggcg gccacgatca gcgacgtccg ctatgatctc acgctcgatg taagtccgct
     6001 agatagtgca ataggttgca taacgatccg cttccaaaag tccgattctg gcgacgccat
     6061 tatcgacttt cggggccgcc gtcttacccg ggcggtggcc aacggccgcc cgattccggc
     6121 tggcaccgcc gcgaacggcc acatactaat ccctgcccgg ctgctcaggg tcggacagaa
     6181 ccagctcgac ttcgccttcg tcgccgacat tgcgccgagc ggcgccagca tcatccgaag
     6241 ccacga
//
LOCUS       DASRJQ010000471         3603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3448009, whole genome shotgun sequence.
ACCESSION   DASRJQ010000471 DASRJQ010000000
VERSION     DASRJQ010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3448009"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VFB89_10710"
     CDS             complement(<1..255)
                     /locus_tag="VFB89_10710"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HZM27820.1"
                     /translation="MIREDEVTADRLATYSDAVFAVIVTVMVLELRAPEEPAFSALWP
                     LWPTAISYGVSYLFIAIIWINHHYLMRFVGPPTLRLIWINF"
     gene            complement(252..722)
                     /locus_tag="VFB89_10715"
     CDS             complement(252..722)
                     /locus_tag="VFB89_10715"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZM27821.1"
                     /translation="MPKPSPRAGRPISLGCACADLRRATRAVTQLYDAALRPEGLRAT
                     QFTLLQALDRAGTITQGVLADVTATDSTTLSRTLGLLEEARWIRSFPGGDRRERHIEL
                     TPAGRRVLERATPAWMSVQQQLRESLGESGWQALESVTAATVVAARERDDTPRR"
     gene            937..1722
                     /locus_tag="VFB89_10720"
     CDS             937..1722
                     /locus_tag="VFB89_10720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZM27822.1"
                     /translation="MEDFSKRWALVTGASSGFGVHFAHLLAERKANLVLVARRTEPME
                     TLATELRQRHGVQVVVQGADLSRTGAAADLKARLDGQGIAVEILVNNAGYGLYGAFLD
                     QPLEKINDMLRLNMITVTELTYLFGRDMAQRHSGHILLIASLLAYQAVPGFAAYAASK
                     AYVLLLGEALHQELAPHGVAVTALCPNASETSFVKVAGQKISPLLRMMMVQPERVARA
                     GVGAMLRRKATVVPGFFTKATVFFDRLMPRSMQRLIVGKVVAG"
     gene            1761..3212
                     /locus_tag="VFB89_10725"
     CDS             1761..3212
                     /locus_tag="VFB89_10725"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27823.1"
                     /translation="MWHPDPFTKYTIPETHPPSAVEAARSLWFSPLSSGRLELIERTW
                     VPAMVPWRATDDGFVTPEVLAWYERFARGQPGALVVEATGIRDIPSGPLLRIGDDRFI
                     PGLAELVRTVRQSSGGRTRLFIQIIDFLRIRRRPEPARYFRDFLQITDGHRAELAGTD
                     DEIREALARMPEAEWPRFLSERELDSLQFGEREQVTDTELPHIRELPRVLPDLFARAA
                     ARAEEAGFDGVELHYAHAYTMASFLSALNQRDDGYGGPRENRVRLPLEVYGAVRDAVS
                     DRFVVGCRFLGDDCIPGGNTAEDAAWFGAEFARAGMDYLSLSRGGKFEDAKQPRIGWA
                     RYPYTGPSGYECMPTVRSDERGPFGRNREATRAVRDTVRASGFETPVVLSGGISTFEQ
                     AEDLLRTGAADIIASARQSLADPDWFRKLRLGLGHEVRRCVFTNYCEGLDQVHKPVTC
                     KLWDHLEVEGDETPRTADGRRRMVAPAWEPATR"
     gene            3209..>3603
                     /locus_tag="VFB89_10730"
     CDS             3209..>3603
                     /locus_tag="VFB89_10730"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZM27824.1"
                     /translation="MRVAIVGAGPAGLYLAILLKRDNPRHQVTVYERNRVEDTFGFGV
                     VFSDETLENVGEADPETNQAMAAAAARWEDIEIHYRGTITRSTGHRFSGVERKTLLEL
                     LAQRAQSLGAEVHWQTEIRDLSDCGLPTA"
BASE COUNT          638 a         1133 c         1208 g          624 t
ORIGIN      
        1 aaagttgatc cagatcagtc gcagtgtcgg aggcccgacg aaccgcatca ggtaatggtg
       61 gtttatccag ataatggcga tgaagagata gctcacccca tagctaatgg ccgtaggcca
      121 tagaggccag agagctgaaa aggccggctc ctccggcgcc ctgagctcca gcaccattac
      181 ggtcacgatc accgcgaaaa cggcgtctga atatgtggcc agccgatccg cggttacctc
      241 gtcttcacga atcatctacg aggcgtatcg tcccgctccc gcgcggccac cacggtcgcc
      301 gcggtcacgc tttccagcgc ctgccaaccg gactctccca ggctctccct caattgctgt
      361 tggaccgaca tccaggccgg cgtggcacgc tccaggactc tgcggccggc cggcgtcagc
      421 tcgatgtgac gctcacgtcg gtcgccgcca ggaaagctgc ggatccagcg tgcttcctcg
      481 agcaggccga gggtgcggct caaggttgtg ctgtccgtcg ccgtaacatc cgccaacaca
      541 ccctgagtga tggtgcccgc tcggtccagc gcctggagca acgtgaactg ggtggcgcga
      601 agcccttccg ggcggagcgc cgcatcatag agctgagtga cggcccgggt ggcgcggcgg
      661 agatcggcgc aggcacagcc caacgagata gggcggccgg cgcgaggcga gggtttaggc
      721 atgaaacaat gctcctacat ccatccgttc aacacaaggc gtgccggtcg ggtaacggat
      781 cgggcggtgg ctgacctcac tgcacatgcg ggccactcag gcgccgggca ggctgcttga
      841 cagggcgata cgaatgtata tacatctagt cgggaaaggt cgagggcgca agacggcact
      901 gtcggggcga tattgccctg atgcaggagg tggcgcatgg aagacttcag caaacgatgg
      961 gccctggtga ccggggcgtc cagtggattc ggcgtccact ttgcccacct gctggccgag
     1021 cggaaggcga atctcgtcct ggtggcacgc cggaccgagc caatggaaac cctggcgacc
     1081 gagctcagac agcgacacgg cgtgcaggtc gtggtgcagg gagcggactt gtcgcggacc
     1141 ggggcggcag ccgatctcaa ggcgcggctg gacgggcagg ggattgcggt cgaaatcctg
     1201 gtgaacaacg cgggctacgg gctgtacgga gcgtttctcg accagccgct ggaaaagatc
     1261 aacgacatgc tccggttgaa catgatcaca gtgacggagc tgacctacct cttcgggcgc
     1321 gacatggcgc aacgccacag cgggcacatc ctcttgatcg ccagccttct cgcgtatcaa
     1381 gcggtgcccg gctttgccgc ctatgccgca agtaaggcgt acgtgctgct gctcggagaa
     1441 gcgctgcatc aggagctcgc gcctcatggc gtagcggtca cggcgctctg cccgaacgcc
     1501 tccgagacgt ctttcgtcaa ggtcgccggc cagaagattt cgccgctgct gagaatgatg
     1561 atggtccagc ccgagcgtgt cgccagggcc ggggtcgggg ccatgttgcg tcgcaaagcg
     1621 accgtggttc cgggcttctt caccaaggcc actgtgtttt tcgaccggct gatgccacgg
     1681 tcgatgcagc ggttgatcgt gggaaaggtg gttgccggct gaccagcggg gtgacccgtg
     1741 cctgatgcat atttttcggc atgtggcacc ccgatccgtt caccaagtac accatcccgg
     1801 aaactcatcc acccagtgcg gtggaggccg cccggtctct ctggttcagc ccgctcagtt
     1861 caggacgcct ggagctgatc gagcgcacct gggtaccggc catggtgccc tggcgtgcca
     1921 cggacgacgg gttcgtcacg ccggaggtcc tggcctggta cgagcgcttc gctcgggggc
     1981 agcccggcgc gctggtggtc gaggcgacgg gaatccgcga catccccagt gggccgttgc
     2041 tcagaatcgg cgacgaccga ttcatccccg gactggccga gttggtgcgc accgtccgcc
     2101 aatcgagcgg cggccggact cgactcttca tccagatcat cgactttctc cgcatccgcc
     2161 gtcggcccga gccggctcga tattttcgcg acttcctgca gatcaccgat ggccaccgcg
     2221 ccgagctggc cggcaccgac gacgaaatcc gcgaggcgct ggcacgaatg ccggaggctg
     2281 agtggcctcg ctttctgagt gagcgcgagc tggacagctt gcagttcggc gagcgggaac
     2341 aggttaccga taccgagttg ccccacattc gcgagctgcc ccgtgtactg ccggatctct
     2401 ttgcgcgcgc tgctgcccgc gccgaggagg cggggttcga tggcgtggag ctgcactacg
     2461 ctcatgcgta caccatggcc tcgttcctct cggctctcaa ccagcgggat gacggctacg
     2521 gtggaccgcg agagaatcga gtgaggctgc cgctcgaggt ctatggggct gtccgtgatg
     2581 cggtgagcga ccggttcgtg gtcggctgcc ggtttctggg cgacgattgc atccccggcg
     2641 ggaacaccgc cgaggacgcg gcctggttcg gagcggagtt cgcccgggca gggatggact
     2701 acctctcgct gtcgcgtgga ggcaagttcg aagacgccaa gcagcccaga ataggttggg
     2761 ctcgatatcc ctacacgggc cccagcgggt acgagtgcat gccgacggtt cgctcggatg
     2821 agcggggacc cttcggccgg aaccgggaag cgacgcgcgc ggtgcgcgac acagtacgcg
     2881 cctcgggatt cgagacgccg gtggtcctgt ccggcgggat ctcgacgttc gagcaggctg
     2941 aggacctgct tcgaaccggt gcagccgaca tcatcgcctc cgcccgccag agcctggcgg
     3001 accccgactg gttccggaag ctccggctcg gcctgggcca cgaggtgcga cgctgtgtct
     3061 tcaccaatta ctgcgagggc ctggatcagg tgcacaagcc ggtgacctgt aagctctggg
     3121 atcatctcga ggtcgagggc gacgagacgc cgcgtactgc tgatgggcgg cgccgcatgg
     3181 tggcgccggc gtgggagccc gcgacccgat gagagtcgcg atcgtaggcg cgggtcccgc
     3241 cggcctctat ctcgccattc tgctcaagcg cgacaatcct cgtcatcagg tgacggtgta
     3301 cgagcggaat cgggtcgaag acaccttcgg ctttggtgtg gttttctccg acgagaccct
     3361 cgaaaacgtg ggagaagccg accccgagac caaccaggcg atggcggccg cagcggcgcg
     3421 ctgggaggac atcgagatcc actatcgcgg cacgatcacg cggtctaccg gacatcgatt
     3481 ctccggcgtc gaaaggaaga cgctgctcga gctgcttgcc caacgggctc aatcactcgg
     3541 cgctgaagtc cattggcaga ccgagatccg cgacctgtcc gactgcggcc ttcccaccgc
     3601 cga
//
LOCUS       DASRJQ010000472         3754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2484032, whole genome shotgun sequence.
ACCESSION   DASRJQ010000472 DASRJQ010000000
VERSION     DASRJQ010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2484032"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..938)
                     /locus_tag="VFB89_10735"
     CDS             complement(144..938)
                     /locus_tag="VFB89_10735"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HZM27825.1"
                     /translation="MAPCPAQVEVLHAVQWRDLLPLRRSEVAGELALSLPWLACSLFA
                     AATGHELVALACSFMFFLTGLRQVHNAFHNALGLSRAGSDWVMLVLSVLMLGSMHAVQ
                     VNHLRHHRHCMAEDDIEAMGARRSALGAMLLGPWFPWCLHRKALEVGTPIQRRWILLE
                     LLANCAWIGAVFLLDYWPLQYHVLAMATAQCLTAFFAVWTTHHGCAPDGPIARTIRNR
                     FKATIAYDMFYHFEHHMFPAVPTCRLAILAGRIDAVVPELRMRYVY"
     gene            991..1590
                     /locus_tag="VFB89_10740"
     CDS             991..1590
                     /locus_tag="VFB89_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27826.1"
                     /translation="MRSKPVFAVSLVAAALSLALSGCGDKQESKPAATAAVRPPAAPA
                     PAPAPAADPLGPRYEASMAEGIDFSKPGYPTFLAEVQGMSGREDWGRWTDGKLAPAAR
                     FKFKQPLPKTFTLVIKAGVFGPNLGKPVAVRVGGVEKTFVHKDNAKAETYRLSFELPG
                     VADTVEIVPPQPTSPKDQNPANQDQRKLGVAISRLQFDN"
     gene            complement(1591..2763)
                     /locus_tag="VFB89_10745"
     CDS             complement(1591..2763)
                     /locus_tag="VFB89_10745"
                     /inference="COORDINATES: protein motif:HMM:NF028132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27827.1"
                     /translation="MSRLSHRLWLACCIFLVALLALPTLQWATRLVPEGGLYGYTDAV
                     PGPPIKPAGAFFNKTLQPWVKEYFDVNLGFRAFFVRTFNEITFFLFGETNRLRMVVTR
                     EHGIHSHLSINFLNEELTRRAELETRYRAEAQKLLRAQKMLEARGKYLEVVIATSKSY
                     AYPEELGRRYLAGDSDGIFSRVASFGDALREAGVNVVDSGPVLRKFARDSGMETHPVP
                     GLHWNFYAGCLIARRILDNARERRFPATPNLDCGAPQFEVRRHTSDYDGLNLLNIWSN
                     GGLLRKSPHPSIVPIREAAWRPNIVFIGDSFSFQIRLALQQGGIYSKLVLANYFAVRE
                     VDGPAAAGETSPAAASVRDQLNADILASDIIILQMVDYNVPRYGYGFADNVIEKLN"
     gene            complement(2767..>3754)
                     /locus_tag="VFB89_10750"
     CDS             complement(2767..>3754)
                     /locus_tag="VFB89_10750"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HZM27828.1"
                     /translation="FLFPQLIAGPIIRYHDIGSQIQHRTHTVEGFLSGFFRFSVGMAK
                     KLIIADPMGGVADKIFALPADGVSFAFAWGGVMAYTLQIYFDFSGYSDMAVGLGRMMG
                     FRFPENFNRPYISRSVTEFWRRWHISLSNWMRVYLYIPLGGNRHSEARTYANLWIVFL
                     ISGFWHGASWNFVAWGAYYGLFLSLERAAAVHERFRIALPALLQQAITVLIVMVGWVF
                     FRSPTLTYAVKYIGAMFGVEGSGAALLTPWGFIFGNREMVTLVIACLIAFVPLPAAWS
                     PWRSLQASWSGATGSRGSATLGFEFVATALLLFLCSSAIVSSDYAPFLYFRF"
BASE COUNT          724 a         1182 c         1260 g          588 t
ORIGIN      
        1 agatcccacc cgagccgctg ggcgaggcgc gcggcgacgg tcgacttgcc gcagccggaa
       61 acacccatga cgacaatcgc gagcacggac ggcttcagcg ccacggaatc actttcagct
      121 cccgctcgtc gcggatctca ccgctagtac acgtatctca tccgaagctc cggcaccacc
      181 gcgtcgattc ttcccgcgag gatcgcgagc ctgcaggtcg gcacggcggg gaacatgtgg
      241 tgctcgaagt gatagaacat gtcgtacgcg atcgtcgcct tgaaccggtt gcggatcgtc
      301 cgtgcgatgg ggccatccgg cgcgcaaccg tggtgcgtgg tccagaccgc gaagaatgcg
      361 gtgaggcact gggccgtcgc catggcgagc acgtggtatt gcagtggcca atagtcgagc
      421 aggaaaacgg cgccgatcca ggcgcagttc gccaggagct cgagaaggat ccaccgacgc
      481 tggatgggtg tgccgacctc gagcgccttg cggtgcaggc accacgggaa ccacgggccg
      541 agcagcatgg cgcccagggc cgagcggcgg gctcccatcg cctcgatatc gtcctcggcc
      601 atgcagtgcc gatggtgccg caggtgattg acctgaacgg catgcatgga accgagcatg
      661 agcacgctca gtaccagcat gacccagtcc gacccggcgc gcgaaagccc aagcgcgttg
      721 tggaaggcgt tgtgcacctg gcgcaggccg gtcaggaaga acatgaagga acaggccagc
      781 gcgaccagct cgtgcccggt ggccgccgcg aagagggagc aagcgagcca tggcagggag
      841 agggccagct cgccggcgac ttcgctcctg cgtagcggca gcaggtcgcg ccattgcact
      901 gcgtgcagga cttcgacttg cgcaggacat ggggccatgg gcgagaagga tatatccttg
      961 tcgccagccc atgttcagtc ggaggatttg atgagatcga agcccgtttt cgcagtgtcc
     1021 ctggttgccg ccgcgttgtc gctggccctt tccggctgcg gcgacaagca ggagtccaaa
     1081 ccggcggcga cggctgccgt ccggccgcca gccgctccag ctcctgcccc cgcccccgcc
     1141 gccgacccgc tcgggccgcg gtacgaggca tcgatggccg agggcatcga cttttccaag
     1201 cccggatatc ccacgttcct ggccgaggtc cagggaatgt ccggccgcga ggactggggg
     1261 cggtggacgg atggcaagct cgctcccgcg gcaagattca agttcaagca gccgttgccc
     1321 aagactttca cgctcgtgat caaggcaggc gtgttcggtc cgaacctggg caagcccgtg
     1381 gcagtgcgcg taggcggcgt cgagaagacc ttcgtccaca aggacaacgc gaaagcggag
     1441 acgtatcgcc tgtccttcga gctgccgggc gtggccgata ccgtcgagat cgtgccaccc
     1501 cagccgacca gtccgaagga ccagaatccc gccaaccagg accagcgcaa gctcggggtc
     1561 gcgatcagcc ggctgcaatt cgacaactga tcagttcagc ttctcgatca cgttgtcggc
     1621 gaagccgtat ccgtatctcg gcacgttgta gtccaccatc tggagaatga tgatgtcgct
     1681 cgccaggatg tcggcgttca gctggtcgcg aacgctggct gcggcgggcg acgtctcgcc
     1741 ggccgccgca gggccgtcga cctcacgcac ggcgaagtag ttggccagga ccagcttcga
     1801 atagatgccg ccttgctgga gcgcgaggcg tatctggaat gagaagctgt cgccgatgaa
     1861 gacgatgttc ggccgccatg ccgcctcccg gatcggcacg atcgacggat ggggtgactt
     1921 cctgagcagc ccgccgttgg accagatgtt cagcaggttg aggccgtcgt agtcgctcgt
     1981 gtggcggcgg acttcgaact gcggggcgcc gcagtccagg ttcggcgtag ccggaaaccg
     2041 gcgctcgcgg gcattgtcca ggatccggcg cgcgatcagg cagccggcat agaaattcca
     2101 atgcagcccc gggacggggt gtgtctccat cccggagtcg cgggcgaact tgcgcagcac
     2161 cgggccggaa tcgacgacgt tgacccccgc ctccctcagc gcatccccga agctcgccac
     2221 gcgcgagaag atgccgtcgc tgtcccctgc caggtacctc ctgccaagct cttcgggata
     2281 cgcgtagctc ttgctggtgg cgatcacgac ttccaggtac tttccccgcg cctcgagcat
     2341 tttctgcgcg cgcaggagct tctgcgcctc ggcccggtag cgcgtctcca gctccgccct
     2401 gcgcgtcagc tcctcgttga ggaagttgat cgagaggtgg ctgtgtattc cgtgctctcg
     2461 cgtcaccacc atgcgcaggc ggttggtctc gccgaacagg aagaaggtga tctcgttgaa
     2521 cgtccgcacg aagaacgcgc gaaacccgag gttgacgtcg aagtattcct tcacccaggg
     2581 ttgcagggtc ttgttgaaga aggcccctgc gggcttgatc ggggggccgg gaacggcgtc
     2641 ggtgtatccg tagaggccgc cctccggaac caggcgcgtc gcccactgga gcgtcggcag
     2701 ggccagcaac gccacgagga agatgcaaca cgcaagccag agtcgatggg aaagccggct
     2761 catgggtcag aacctgaagt agaggaacgg ggcatagtcg gagctcacga tcgccgacga
     2821 gcacaggaag agcagcagtg cggtcgccac gaactcgaat cccagcgtgg ccgagccgcg
     2881 gctcccggtt gcgcctgacc aggaggcctg gagcgaccgc cacggggacc aggcggccgg
     2941 aaggggcacg aaggcgatca ggcaggcgat caccagggtc accatctccc gattgccgaa
     3001 gatgaagccc cacggcgtga gcagcgcagc ccccgagccc tcgacgccga acatggcccc
     3061 gatgtacttc accgcgtagg tgagggttgg cgagcggaag aacacccaac ccaccatgac
     3121 gatcaacacc gtgatggctt gctggaggag ggctggaagc gcgattcgaa acctctcgtg
     3181 caccgccgcg gcccgctcga ggctcaggaa gagcccgtag taggcgcccc acgcgacgaa
     3241 attccagctc gcgccatgcc agaaaccgga gatcaggaac acgatccaga ggttggcgta
     3301 ggtgcgggcc tcggagtgcc tgttcccgcc cagcggaatg tagaggtaga cccgcatcca
     3361 gttggacagg gaaatgtgcc agcggcgcca gaactcggtg acgctcctgg aaatgtaggg
     3421 gcggttgaag ttctcgggaa accggaaacc catcatgcgg cccaggccga ccgccatgtc
     3481 cgaatagccc gagaaatcga aatagatctg cagcgtgtag gccatgacgc cgccccacgc
     3541 aaacgcgaag gagactccgt cggccggcag cgcgaagatc ttgtcggcca cgccgcccat
     3601 cgggtcggcg atgatgagct tcttggccat gccgacgctg aagcggaaga atcccgagag
     3661 gaagccttcg accgtgtggg tgcgatgctg gatctggctg ccgatgtcgt ggtagcggat
     3721 gatcgggccg gcgatcagct gcgggaagag gaag
//
LOCUS       DASRJQ010000473         5833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_576785, whole genome shotgun sequence.
ACCESSION   DASRJQ010000473 DASRJQ010000000
VERSION     DASRJQ010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5833
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576785"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1294
                     /locus_tag="VFB89_10755"
     CDS             <1..1294
                     /locus_tag="VFB89_10755"
                     /inference="COORDINATES: protein motif:HMM:NF023591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27829.1"
                     /translation="AMYAASGEGRYKVYIVDEAHMLTREAWNALLKILEEPPPRVVFV
                     FATTEPQKIGATAAPVLSRLQRFDFRRIGPGAIRGRLREVLAAEDIKAEEDALTLIAQ
                     HADGGMRDALSMLDQCLSFGEDTVTAMRVREVLGLPGDELYAEVLGLVAERRPAAVFP
                     LVDRLVNAGADLAEFLAGAGEALRALLMLQLGAEPEGLTETMRGALLRYRDVLVSGDV
                     LRMLKLLSDNEIAIRRSANSRLMVETLLLRWAMLDRIVDLQQVLEGAPAGRPQPERER
                     RSPPPAAPEPAPAPTNARPVAGSVEFTAEGLRAAWPEIVAAVRERRPFLGEAVATAEP
                     AEVAPPWVTVRVPESGALFVQPLQEHTGQLEQILGQVVGALVRLRVVAGEGGGTVGSE
                     APKRMSEASIRADRLKRLRAKDPALDTAADALDLEIVE"
     gene            1356..1682
                     /locus_tag="VFB89_10760"
     CDS             1356..1682
                     /locus_tag="VFB89_10760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HZM27830.1"
                     /translation="MAGLPEGMADLQQLFQLSQQVQGRLQQLQSELAGRIIETTAGGG
                     LIRVTADGRGTIRAVTIDPTVFQEHDAELLGDLVLSAVAEAQRRAADLLQAEMRKVQP
                     LPFPSL"
     gene            1685..2275
                     /gene="recR"
                     /locus_tag="VFB89_10765"
     CDS             1685..2275
                     /gene="recR"
                     /locus_tag="VFB89_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HZM27831.1"
                     /translation="MAAIDQLIAEFAKLPGIGRKTAQRLTFHLLQQSPEQASRLAAAL
                     SSVAERVRPCPECGNPTEDPMCSICADPRRDPTLLCVVEEASAVSVVERATQFQGRYH
                     VLGGRLSPLDGVGPEALRLDRLVERVRPGQVREVILATNPSMEGEVTATYIQQLLAGL
                     GVRVTRLARGLPMGGDLEYVDGVTLAHALSARQEVP"
     gene            2275..2544
                     /locus_tag="VFB89_10770"
     CDS             2275..2544
                     /locus_tag="VFB89_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27832.1"
                     /translation="MRSRAALFAGGAAAGLIAGWLVAQYRFTYHRADLFSGQPLRRLA
                     ALGFLAGQNDIETIRLLRDYLAWETHPVLRRRADGIMRRMEEAFL"
     gene            2555..3055
                     /locus_tag="VFB89_10775"
     CDS             2555..3055
                     /locus_tag="VFB89_10775"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HZM27833.1"
                     /translation="MTVGASSWSFREEDGQRLQAILTRFLRDAGARTALIVDRTGQLV
                     ATVGEAPVFDPTAFASLTAADFSANDQLARMLGEPEFGVLFHQGERESMYLADIARRV
                     ILVVLFDNRTTLGLVKLRVKPAVGELNQLFVAMFERDGAGASGPGMATEFVGEAEDEI
                     DKLFGA"
     gene            3071..3745
                     /locus_tag="VFB89_10780"
     CDS             3071..3745
                     /locus_tag="VFB89_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HZM27834.1"
                     /translation="MSMINYASREINCKLVYYGPGLGGKTTNLEHVYNKVAPGSRGKM
                     ISLATETERTLFFDFLPVDLGSVRGFKVKFHLYTVPGQVYYNASRKLILKGVDGVVFV
                     ADSQIDRMDANIEAMQNLYENMAQYGYDMTRIPFAVQYNKRDLPNAAPIPELQAALNP
                     GWPVSDAGRQRLTPDPYHEGEYLTYQQDGQWIERAPYFEAVALTGDGVFDTLRAISKS
                     VVKALG"
     gene            3754..4392
                     /gene="pgsA"
                     /locus_tag="VFB89_10785"
     CDS             3754..4392
                     /gene="pgsA"
                     /locus_tag="VFB89_10785"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HZM27835.1"
                     /translation="MWTLPNIITIVRICFTPVIALLPFIAGYWPKLICFVIFVVAAVS
                     DVFDGYLARSRKQVTDLGILLDPIADKLLLFSTLIPIYWISRQRHDLYDIPLWGSIPL
                     WVCVLLIGRELAMTGFRWWAKRRGVIIPAGGAGKLKTVIQNIFIGATIAWFAFRDARK
                     PLGWEHSRFADYWNEFHGSFVAVTLAIAALLTVYSFATYIYHHRHLFSARAR"
     gene            4443..5699
                     /locus_tag="VFB89_10790"
     CDS             4443..5699
                     /locus_tag="VFB89_10790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HZM27836.1"
                     /translation="MNIEIVTIGTELLLGYTLDSNGAEIARALGEHGVRVVRRTSVGD
                     RGPEIGDAVRDALARTGAVLTTGGLGPTRDDVSKRAVAELLGVPLRFDDSVWDALVAR
                     FARLGRVPVASNRVQAEVPEGAVVLPNRWGTAPGLWIDSDKLGGRDGARGLVIMLPGV
                     PREMRNLLQHEVIPRLVEADSGTVVRSLTVRTTGIPESTLAERLGEIESEIAPLTLAY
                     LPGEEGVDLRLTAWALAPDEAERRLRSAGNLLHARAGEHVYGEGDTDLAGLVLEGARS
                     KSLHIAAAESCTGGLLAKRLTDTPGSSDVFRGGVVVYDNALKTSLLDVPPELIERHGA
                     VSEEVARAMAVGALARLDADLAAAVTGIAGPGGGTPEKPVGTVWFATALGEDVAAVRS
                     VVPGSRSEIRARAAQAALYLLLRRLR"
BASE COUNT          964 a         1848 c         1929 g         1092 t
ORIGIN      
        1 ggccatgtat gctgcctcgg gcgaagggcg ttacaaagtc tatatcgtgg acgaagccca
       61 catgctcacc cgagaggcct ggaacgccct cctcaagatc ctggaggagc cgcccccacg
      121 cgtcgtcttc gtcttcgcga ccaccgagcc gcagaaaatc ggcgcgaccg ctgcaccggt
      181 gctctcccgt cttcaacgtt ttgacttccg gcggatcggc cccggcgcga tccgcgggcg
      241 gctgagagag gtccttgccg ccgaagatat caaggccgag gaagacgccc tcacgctgat
      301 cgcgcagcac gcggacggcg gcatgcgcga cgctctctcg atgttggacc agtgcctgag
      361 cttcggcgaa gacacggtga ccgctatgcg agtacgcgag gtgctcggac ttcccggcga
      421 cgagctctac gccgaagtac tcggcctcgt ggcagagcgg cggccggctg ccgtctttcc
      481 gctcgtggat cggctggtga atgcgggagc cgatctggcc gagtttctcg cgggggcggg
      541 agaagcgctg cgggcgctcc tcatgcttca gctcggcgcc gagcccgaag gcctcaccga
      601 gaccatgcgc ggcgccttgc tccgatatcg cgacgtactc gtttccggag acgtgcttcg
      661 gatgttgaaa ctgctctccg acaacgaaat tgcgattcgg cggagcgcca acagccgcct
      721 catggttgaa acgctgctgc tccgctgggc catgctcgat cggatcgtag acttgcagca
      781 ggtgctcgag ggtgcgcctg ccggtcgtcc gcagccggaa cgcgagcgca gatctccgcc
      841 accagccgct cccgaaccgg cgccggctcc aacgaatgcg aggccggttg ccggatcggt
      901 cgaattcacc gctgaaggcc ttcgggccgc ctggcccgag atcgtcgccg cggtgcgcga
      961 gcgccgcccc ttcctgggtg aggccgtcgc cacggccgaa ccggccgaag tcgctccacc
     1021 ttgggtcacg gtacgagtac ccgaatcggg ggctctgttc gtgcagcctc tccaagagca
     1081 cacggggcag ctggaacaga tcctgggaca ggtcgtcgga gctctggtca ggctccgggt
     1141 ggtggccggg gagggcggcg gaaccgtggg ttcggaggct cccaaacgca tgtcggaggc
     1201 gtcaattcgg gcggatcgcc tgaagcggct tcgagccaag gaccccgcgc tagacactgc
     1261 ggcggacgcg ttagatttgg agatagtgga gtaacgaggc tggcagcgat attgcggctt
     1321 gcggccgact ttttgcagac ctactttgcg cccatatggc aggactaccc gaaggcatgg
     1381 ctgatctcca gcaacttttc cagctgagcc agcaggtcca gggccggcta cagcagctgc
     1441 agtccgagct cgcgggacga atcatcgaaa caacggccgg cggcggtctc atccgggtca
     1501 cggccgacgg acgcggcacc atccgggccg tcaccatcga ccccacggta ttccaggagc
     1561 atgacgcgga gctgttgggc gacttggtgc tgtccgcggt agcagaggcc cagcggcgcg
     1621 cggccgacct gctccaggcc gagatgcgga aggtgcagcc gttgcccttc ccttcgctct
     1681 gacggtggcc gcgatagatc agctgatcgc cgagtttgcc aagctgcccg ggatcggccg
     1741 caagacagcc cagcgcctca cctttcatct gttacagcaa tcccccgagc aagccagccg
     1801 cctggcggct gcgttgtcct cggtggccga gcgggtgcgg ccctgtccgg aatgcggcaa
     1861 cccgactgaa gacccgatgt gctcgatctg cgccgatcct cggcgggatc cgacgctgct
     1921 gtgtgtcgtc gaggaggcgt ccgcggtctc cgtcgttgag cgcgccaccc agtttcaagg
     1981 ccgctaccac gtgctgggcg gccggctctc tcccctcgat ggagtcggcc cggaagcgtt
     2041 gcgccttgac cgactggtag agcgagttcg gcctggtcag gttcgggagg taatcctggc
     2101 taccaatccg tcgatggaag gcgaagtgac tgcgacctac atccagcaac tgctggctgg
     2161 cttgggtgtg cgggtgaccc gcctggcccg cgggctgccg atgggtggag acttggaata
     2221 tgtcgatggc gtcacgctcg ctcacgcgct gagcgctcgc caggaggtgc cctgatgcgt
     2281 tcccgtgccg ccctctttgc cggcggtgca gccgccgggc tcatcgccgg ctggttggtc
     2341 gcccagtatc gcttcacgta ccatcgtgcg gatctgttca gtggccagcc cctccgtcgc
     2401 ctggccgcgc tcggctttct cgccggacaa aacgatatcg agaccatccg cctgctccgc
     2461 gattatctcg cctgggaaac ccatcccgtg cttcgccgcc gggcggatgg aatcatgcga
     2521 cggatggaag aagcgttctt atgagacgcc cgctgtgacc gtgggggcgt ccagctggtc
     2581 cttccgcgag gaggacggac agcgcctgca ggcgatcctg acccgatttc ttcgggacgc
     2641 cggcgcgcgg acggcgctca ttgtcgaccg tacggggcag ctggtcgcca cggtgggtga
     2701 ggctccggtg ttcgatccga ccgccttcgc gtcgctcacc gccgccgact ttagcgcgaa
     2761 cgatcagctg gcccgcatgc tgggcgagcc cgagttcggc gtcctgtttc accagggtga
     2821 gcgggaatcg atgtacctcg ccgatattgc ccgccgggtg atcctggtcg tgctgttcga
     2881 caaccggacg actctcggac tggtcaagct tcgggtcaag ccggcggtgg gagagctcaa
     2941 tcagctgttt gtcgccatgt tcgagcgtga tggagccggt gcgagcggac ctggcatggc
     3001 gaccgagttc gtaggtgagg ccgaggatga gatcgacaag ctcttcggtg cctgagggag
     3061 acgcggccgc atgtcgatga tcaactacgc gtcccgggag atcaactgca agttggtcta
     3121 ctacgggccc gggctgggcg ggaagacgac caacctcgag cacgtataca acaaggtggc
     3181 ccccggctcc cgggggaaga tgatttctct ggcgactgag actgaacgga ctctgttctt
     3241 cgattttctg cccgtcgatc tcgggtcggt ccgcggattc aaagtaaagt tccatctgta
     3301 caccgtcccc ggccaggtct actacaacgc atcccgcaag ctgatcctca agggtgtgga
     3361 cggtgtcgtg ttcgtcgccg atagccagat cgaccggatg gacgcgaaca tcgaggccat
     3421 gcagaacctc tacgagaaca tggcgcagta cgggtacgac atgacccgta tcccgtttgc
     3481 cgtccagtac aacaaacggg atctccccaa cgcggccccg attcccgagc tgcaggcggc
     3541 actcaacccg ggatggccgg tgagcgacgc aggacgtcag cgcctgacac cggacccgta
     3601 tcacgaaggc gagtacctga cgtaccagca ggacggtcag tggatcgagc gggcccccta
     3661 cttcgaggcc gtcgcattga ctggcgatgg cgtgttcgac accttgcgcg cgatcagcaa
     3721 gagcgtcgtg aaggcgctgg gataggggcg accatgtgga cgctacccaa catcatcacg
     3781 atcgtgcgga tctgcttcac accggtcatc gcgctgctgc cgttcattgc cggctactgg
     3841 cccaagctca tctgcttcgt gattttcgtg gtggccgcag taagcgacgt cttcgatggc
     3901 tatctcgccc gctcccgtaa gcaagtcacc gatctcggta tcctcctcga tcccattgcc
     3961 gacaaactgc tgctcttctc taccctgatc cccatttact ggatctcgcg ccagcggcac
     4021 gatctctacg acatccctct ttggggcagc atcccgcttt gggtttgcgt gctgctgatc
     4081 ggaagagagt tggccatgac cggttttcgg tggtgggcca agcgccgcgg cgtgatcatc
     4141 cccgcagggg gtgccggaaa gctcaagacg gtcattcaga acatcttcat cggggcgacc
     4201 attgcgtggt tcgcattccg cgacgcgcgc aagccgctcg gctgggagca cagccgtttc
     4261 gccgactatt ggaacgagtt ccacggcagc ttcgtggcgg tgactctcgc gatcgcggcc
     4321 ctgctcacgg tgtattcctt cgctacctac atctaccatc accgacacct gttctccgct
     4381 cgcgctcgct gataccactt ccggctcgcc ttcgccccgc caggtagtgc atccttaaag
     4441 gcatgaatat cgagatcgtc acgattggta ccgagctgct gcttggctac acgctcgaca
     4501 gcaacggtgc cgaaatcgcc cgggccctgg gcgagcacgg ggtgcgtgtg gtacgccgga
     4561 cctcggtggg cgaccggggt ccagagattg gcgacgcggt ccgtgatgcg cttgctcgca
     4621 ccggagccgt gctcaccaca ggtggtctcg gccccacccg ggacgatgtc agcaagagag
     4681 ccgttgccga gctgctgggc gtacctcttc gattcgacga ctcggtgtgg gatgcgctgg
     4741 ttgcgcgatt tgccaggctc ggtcgggttc cggttgcgtc gaatcgggtt caggcggaag
     4801 tgcctgaggg cgctgtcgtg ctccccaatc gttggggcac ggcaccggga ctgtggatcg
     4861 actcggacaa gttggggggg cgcgacggtg cacgtgggct cgtcatcatg ctgcccggtg
     4921 ttccgcggga aatgcggaat ctgctgcagc acgaggtaat tccccgcctg gtcgaagccg
     4981 attccggcac ggtggtccgg tcgctcaccg tgcgcaccac ggggatcccg gaatcaacct
     5041 tggccgaacg gctcggcgag atcgagtctg aaatcgcgcc gctcacactg gcctacctgc
     5101 ccggtgagga gggagtcgat ctgcggctca cagcctgggc gctcgctccg gacgaggccg
     5161 agcgacggct ccgttcggcg ggcaacctgt tgcacgcgcg ggccggtgag cacgtgtacg
     5221 gcgaaggcga cacggatctg gcaggtctcg tgcttgaggg ggcgcgctcg aagagcctcc
     5281 acatcgccgc agccgagtcg tgcaccggtg ggctcctggc gaagcgtctg accgacacgc
     5341 cgggtagctc ggatgtcttc cgtggcgggg tcgtggtcta cgacaacgct ctcaagacgt
     5401 cgctgctcga cgttccgccg gaacttatcg agaggcacgg tgcggtgagc gaagaggtag
     5461 ctcgcgccat ggcggtcggc gctctcgcgc ggctcgatgc cgacctcgca gccgcggtca
     5521 cggggatcgc cggacccggc ggtggaactc ccgagaagcc ggtgggtacg gtctggtttg
     5581 ccacggccct cggcgaagac gttgccgctg tacggagtgt ggtgccggga tcgcgttccg
     5641 agattcgggc acgagccgct caggctgcgt tgtatctcct gcttcggaga ctgcgctagt
     5701 tagtctgagt gatcaccccc tcagcgaccg ggcgtcatcg cggccagcgc gaagccattg
     5761 ttattttact gcgtcctttc gagacgatga gtgtgcaagt gactgaaaac gagtggttta
     5821 gcagcgaagg tcg
//
LOCUS       DASRJQ010000474         4939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3075440, whole genome shotgun sequence.
ACCESSION   DASRJQ010000474 DASRJQ010000000
VERSION     DASRJQ010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3075440"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VFB89_10795"
     CDS             <1..195
                     /locus_tag="VFB89_10795"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HZM27837.1"
                     /translation="RHTPFTPPSPSRIEVAFTYSYQADYAELVPGSTRLGPVTVAYTH
                     ADFCEVFRAFRAFYNLARGQ"
     gene            348..866
                     /locus_tag="VFB89_10800"
     CDS             348..866
                     /locus_tag="VFB89_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27838.1"
                     /translation="MSARWPIVVISIVGLICTACESDMAAPEESSTKGPSLRASAFTQ
                     VTNFPIDFAVFIPCANGGTGEVIEMSGNLHDVFHATSDGNGGFHVKTHDSPQGISGVG
                     LTTGMKYNATGVTQEEFDIKVGQHDTFVNNFRMIGQGPGNNFAIHENFHVTINANGVI
                     TALHDNFRATCK"
     gene            complement(875..1699)
                     /locus_tag="VFB89_10805"
     CDS             complement(875..1699)
                     /locus_tag="VFB89_10805"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZM27839.1"
                     /translation="MSRRRFLVGGAAILATGLAADAFSVEPRRVQLSRHDVALPGLPP
                     ALDGLTIAQVSDIHLPANRAAVERTLAMLDAERPDIVLLTGDQCETSTAMDDLVVFVR
                     AARGKLATIAILGNWDYRGRVIGLLARRAYESAGAMLLVNQHTVVEVGGARVAFVGLD
                     DMLSGSPNAAAALTGLPPDVPAIWTIHEPAFADYLTPAPTQRPALVLAGHTHGGQIRL
                     PGVPAFTPAGSGRFLEGWYDSTLGRLYVSRGIGTADVRARFRCPPELPIFVLRSAP"
     gene            complement(1844..2560)
                     /locus_tag="VFB89_10810"
     CDS             complement(1844..2560)
                     /locus_tag="VFB89_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27840.1"
                     /translation="MRRYSACARYNDRIIRIAPDRWEGYIDRGRIEVLGRGNVSAGQR
                     LLHEGLEHGDGQRLLALMWLFVVRFGAPEDRDAMLLLPVEAFGSDTMGYYASKGQVWR
                     LRGDSARTRAYYDSLRAYTTERIRRRPQDDAAHTALGYAYARLGRAADAVREGRRGVE
                     LLPVSRDAYEGSERLLDLAKIYAVVGDADAAVDQLSALLEIPSWVSPAWLRVSWDWDE
                     IRDNSRFQQLTHRPLPAETQ"
     gene            complement(2564..4564)
                     /locus_tag="VFB89_10815"
     CDS             complement(2564..4564)
                     /locus_tag="VFB89_10815"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM27841.1"
                     /translation="MSDLRERLQAALGDVYHLERELGGGGMSRVFVAEEVELKRKIVI
                     KVLPPELAAGINTDRFRREVQLAASLQHPHVVPLFTAGSADQLLYYTMPFVKGESLRE
                     RLAREGELPVADAVRILRDVVDALAYAHGEGVVHRDIKPANVLLSGGHALVADFGVAK
                     ALSDAAHGVRLTSAGVAIGTPMYMAPEQAVADSHADHRVDIYAVGALAYEVLTGSPPF
                     AGSVPQQVLAAQLAAPPEPILKRRGAIPPTLAAAVMRCLEKRPADRWQTAEQLLHQIE
                     PLATPSGGTAPHPAISDSSGFRPAVLDPTTPLPATTPTVAPESRKLFGRWLLIAAAVL
                     LAFAAGYAIMRQRAAASGSAGEAAPKSLVVLPFENLGAADDKYFADGITEEITSRLAA
                     VPDLKVTSRTTAMQYKDTKKTVRQIGQELAAGYVLEGSVRWEKAGGQNRIRVTPQLIR
                     VSDDSHIWADRYDAVLAEVFDVQSNIAEKVVAALGVALGEPAKRVLAARPTSNPEAYD
                     YYLRGQEAWNRGEAEWDVRAATQALERAVALDSAFASAWALLSRTNAFIYWQGRDLTP
                     RRLELARSAMERATAIAADAPETQLARGYYRYWGFRDYPAALQEFEAALRSRPEDADI
                     IESIGLIQRRQGRWEDALATLDRASQLEPRSIGLIGDAEETA"
     gene            complement(4684..>4939)
                     /locus_tag="VFB89_10820"
     CDS             complement(4684..>4939)
                     /locus_tag="VFB89_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27842.1"
                     /translation="GRRAAELLPVSRDAFEGADRLMNLALIYAGLADSDDAVRELRAL
                     LEIPSWVAPGDLRLNPQWTLIRNDPRFQQLANQPVAPDEN"
BASE COUNT          942 a         1593 c         1543 g          861 t
ORIGIN      
        1 cggcacacgc cctttactcc ccccagcccg agcaggatcg aggtggcctt cacctattcc
       61 tatcaggccg actacgccga gctcgtgccc gggtcgactc gtctcggacc cgtcaccgtc
      121 gcctacaccc acgccgattt ctgcgaggtg ttccgtgcgt tccgggcctt ctacaatctc
      181 gctcggggtc agtagcacac accacacgat gacgcaagcc tagtccgaac gcacttctgt
      241 ttgtgacaaa tcggcttgac gatcttgccg agtagcgcta cctctagctc gctcgatcct
      301 tcacacaatc ccggtatacc aaagccgctt tccaaacgag gtcccccatg agcgcccgat
      361 ggccgattgt cgtcatatca atcgttggtt tgatctgtac cgcctgcgaa agcgatatgg
      421 cggctcctga ggagtcctcc acgaagggtc caagcctccg agcgagtgct ttcacgcagg
      481 tcacgaactt cccaatcgac ttcgctgttt tcattccctg tgcgaacggc gggactggcg
      541 aggtgattga gatgagcggc aatctgcatg acgtattcca tgcgacatca gacggcaatg
      601 gcggctttca cgtcaagacc cacgatagcc cgcagggaat ctccggggtg ggactgacga
      661 cggggatgaa gtacaacgcg accggtgtca cacaggaaga attcgacatc aaggttggtc
      721 agcacgacac cttcgtcaat aacttccgga tgatcggcca gggacccggg aataactttg
      781 caatacacga aaacttccat gtcactatca acgccaacgg cgtcatcacg gcgttgcatg
      841 acaatttcag ggcgacttgc aagtaggtac gtgatcaagg ggcgctacgc agcacgaaga
      901 tcggaagttc aggtggacag cgaaaccgtg cccgcacatc ggccgtgccg atgccgcgcg
      961 agacgtaaag ccggcccagc gtcgagtcgt accatccttc gagaaagcga ccggatccgg
     1021 caggagtgaa ggctggaact ccaggaaggc gaatctgacc tccgtgggta tggccggcga
     1081 gcaccagcgc cggcctctgc gttggagcgg gcgtgagata gtcagcgaag gccggctcat
     1141 ggatggtcca gatcgcagga acgtcgggtg gcagtcctgt gagagcagcc gcagcgttgg
     1201 gtgagccgga aagcatgtcg tcgaggccta cgaaggcgac cctcgctcct cccacctcaa
     1261 cgacggtgtg ctggttcacc agcaacatcg cgccggcact ctcatatgct cgccgcgcaa
     1321 gcaagccgat cacgcgaccc cggtagtccc aattccccaa aatcgcgatc gtggcgagct
     1381 ttcccctggc cgcgcggacg aacaccacca ggtcgtccat cgcggtgctg gtttcgcact
     1441 gatcaccggt gagcaggacg atatccggcc gttcggcgtc cagcatggcc agcgtgcgct
     1501 cgacagcggc acggttggcc gggaggtgaa tgtcagagac ctgggcgata gtgaggccat
     1561 cgagcgctgg cgggaggccg ggaagcgcga cgtcgtggcg gctgagctgg acgcggcgtg
     1621 gctcgacaga gaacgcatcg gcggcgaggc cggtcgcaag aatcgccgct ccgccaacca
     1681 ggaaacggcg tcgcgacacc acgggagtgc cagagcccga ggcttcggca tgcaacggct
     1741 cagccaaagt ggtgttcttt gggcattctc aaagcccctt cttcaaccgt cgcaacctag
     1801 cagggggcag cgccggaatt accaagccaa aaggcagacc gtcttactgg gtttcagctg
     1861 ggagcggtcg atgagtgagc tgctggaagc gggaattatc ccgaatctca tcccagtccc
     1921 aactgactcg cagccaggca ggcgagaccc acgaggggat ctcgagaagc gcggagagct
     1981 ggtcaacggc ggcgtctgcg tcgccgacca ccgcgtatat cttcgccaag tccagcaacc
     2041 gctcacttcc ttcgtaagca tcacgggaca cggggagtaa ctcgacgccg cggcggccct
     2101 ctcgcacggc atctgcggcc cggccgagtc tggcataggc gtatcccagc gccgtgtggg
     2161 cggcatcgtc ctgcggtcgt cggcgaatcc tctctgtcgt gtacgcccgc aggctatcgt
     2221 agtaggcgcg ggtccgagcc gaatcgcccc gcagtcgcca aacctggccc ttactcgcgt
     2281 agtatcccat cgtgtcggaa ccgaaggctt ccaccggcaa gagaagcatc gcgtcccgat
     2341 cctcaggcgc gccgaagcgg acgacgaaca gccacatgag cgcgagcagt cgctgaccgt
     2401 cgccatgctc cagcccctca tgcagcaggc gctgacctgc tgagacgttc ccacggccga
     2461 gcacctcaat ccttccgcgg tctatgtacc cctcccacct gtcgggggcg atgcgaataa
     2521 tccggtcgtt gtaccgggcg caagctgagt atcgccgcat cattcaggcg gtctcttcag
     2581 cgtcgcctat gagtccgatg gaccgaggct ccagctggct ggcgcggtca agggtggcaa
     2641 gggcgtcctc ccagcgccct tgccgtcgct ggatcaaccc gatggattct atgatgtcgg
     2701 cgtcttcggg ccggctccga agggcggcct cgaactcctg gagagcggcg gggtagtcac
     2761 gaaatcccca gtagcgatag tagccacgcg cgagctgcgt ctccggcgcg tccgcggcga
     2821 ttgcggtggc ccgctccatg gctgatcggg caagctccag gcgacgagga gtcaggtccc
     2881 ggccctgcca ataaatgaac gcattggtcc ggctcaggag cgcccaggct gacgcaaagg
     2941 cggaatccaa tgccacagcg cgctcgagcg cttgagtcgc cgcccgtaca tcccattcgg
     3001 cctccccgcg gttccaagcc tcctggcccc ggaggtagta gtcgtacgct tccgggttgc
     3061 tcgtcggacg cgcagcaaga actcgcttcg caggctcacc tagagcgacg ccaagagccg
     3121 ccacgacctt ctcggcgata ttcgactgta cgtcgaacac ctctgcgagg acggcgtcat
     3181 accgatccgc ccatatatga ctgtcgtccg acacccggat cagctgaggc gtaacgcgga
     3241 tccggttttg gccgccggcc ttttcccagc ggacgctgcc ctcgaggacg taccctgccg
     3301 caagctcctg cccgatctgg cgcacggtct tcttggtgtc tttgtactgc attgccgtcg
     3361 tgcgcgaggt gacctttaga tcgggcacgg cggcaagcct gctggtaatc tcctccgtga
     3421 tcccgtcagc gaagtacttg tcgtcggccg cgccgaggtt ttcgaaaggc agaacgacga
     3481 gcgacttcgg cgctgcttct ccagctgatc ctgacgctgc cgcccgttgg cgcatgatcg
     3541 cataccccgc cgcgaatgcc agcaagactg ccgctgcgat caggagccac cgcccaaaaa
     3601 gcttacggga ctcgggagcg acggtgggag tcgtggccgg taacggagtc gtgggatcaa
     3661 gcacggctgg tcggaatccc gacgagtccg aaatcgcggg atgtggagcc gtgccgccac
     3721 tcggtgttgc gagcggctcg atctggtgaa gcaactgctc cgccgtctgc caccggtctg
     3781 cagggcgctt ctccagacac cgcatcaccg cagcggcgag agttggagga atcgcgcctc
     3841 ggcgcttgag aatcggctca ggcggcgcgg ccagctgtgc ggcgagcacc tgctgcggca
     3901 cggagccggc gaacggcggc gaaccggtca agacttcgta tgcgagggcg ccgacggcgt
     3961 agatgtcgac ccgatggtca gcatgcgaat cggcgaccgc ttgctccggc gccatgtaca
     4021 tcggtgtgcc gatcgccact ccggcggacg ttagcctcac gccgtgggcc gcgtcggaaa
     4081 gcgctttggc gacgccgaaa tccgccacca gcgcgtgtcc gcccgaaagg agcacgttgg
     4141 ccggcttgat gtcgcggtgt accactccct ctccgtgagc gtaggcgagt gcatccacca
     4201 cgtcccgaag aatccggact gcgtcggcga ccggcagctc accctcgcgg gccaatcgct
     4261 cccgaagcga ctcgcctttc acgaacggca tggtgtagta gagcagctgg tccgccgaac
     4321 cagcggtgaa gagaggcact acgtgaggat gctggagact ggccgcaagc tgcacttccc
     4381 gcctaaaccg atcagtattg atcccggcgg cgagctcggg gggcagaacc ttgatcacga
     4441 ttttccgctt gagctcgacc tcctcagcga cgaagacgcg actcatgcca ccgccgccga
     4501 gctcgcgctc gaggtggtag acgtcgccga gcgcggcttg cagccgctcg cggagatcgg
     4561 acagcgggac ggccctctgc gaaggaggta gctaacgata cccatccgcc gtcgggcgag
     4621 ccagcgtgcc ggggggcacg ctagcgtctg acgctgcacg gctttcgttt cggtggggtg
     4681 tggtcagttt tcgtcgggag caaccggctg attggcaagc tgctggaagc ggggatcatt
     4741 ccgtatcagg gtccattgcg gattcagccg aagatctccc ggtgcgaccc acgagggaat
     4801 ctcgagtaga gcgcggagtt ccctaaccgc atcgtcgctg tcagcgagcc cggcataaat
     4861 cagagccaga ttcatcaagc gatcggctcc ctcgaaggcg tccctcgaca ccggaagcaa
     4921 ctcggccgcc cgccgaccc
//
LOCUS       DASRJQ010000475        11313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2887027, whole genome shotgun sequence.
ACCESSION   DASRJQ010000475 DASRJQ010000000
VERSION     DASRJQ010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 11313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11313
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2887027"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VFB89_10825"
     CDS             complement(<1..878)
                     /locus_tag="VFB89_10825"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZM27843.1"
                     /translation="MSTEVALGRPAPASGEHRAAVLAAFLGWTLDAFDFFLVVFALTA
                     IARDFGTSDAAIALSITVTLAFRPVGALIFGLLADRYGRRLPLMLDLVFYSVVEVLSG
                     LAPDYKTFLVLRALFGIGMGGEWGVGASLAMEKAPARLRGVLSGLLQEGYAMGFLLAA
                     ACFYLVYPRFGWRPMFFIGGLPALLALFIRFRVKESEVWERTKHANWRELGQGIVSHW
                     RLFLYLTVLMASMNFASHGTQDMYPTFLERQWGFGPQSRAGLTAFSMVGAIIGGVAFG
                     LFSDRIGRRRAIVLAL"
     gene            1007..2056
                     /locus_tag="VFB89_10830"
     CDS             1007..2056
                     /locus_tag="VFB89_10830"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZM27844.1"
                     /translation="MRIALISTPFVSAPPLDYGGTELVVYELAEGLVEQGHHVALFAT
                     GDSKTSADLRALFPTAQWPPNTLSDVHHLSWALQQIAQQHFDIVHAHSAAALAIARLI
                     SLPPLVYTIHHNRNEVLSAFYPYFPEVHFVAISANQQSREVPLERCTVIHHGLDPTRF
                     KLTEHPSDYVCFVGRFSPEKAPHLAIEAAQKAEVPIRVAGRTHAPDAHYAAKELEPRL
                     MLPEVEDQGPVGMKEKSELLTHARALLAPVQWEEPFGLILVEAMMSGCPVVAFPRGSI
                     PELVDPGETGIIVNSLEEMTDAIRPGGPVDRIDRRRCRTRAVTRFSRQRMVREYEQLY
                     QRVLWETKPHLPITA"
     gene            2020..4281
                     /locus_tag="VFB89_10835"
     CDS             2020..4281
                     /locus_tag="VFB89_10835"
                     /inference="COORDINATES: protein motif:HMM:NF026092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HZM27845.1"
                     /translation="MGDQTTPADHRVSRTVEPVPTQIGPAPAFEGHRISMEEAVGYGA
                     IDCTHMSPPDSTLILKQDRLFLVMTVRGEIAPPGHCSLGLFHDDTRILSHYALRVRGG
                     APAFLSADAPRTYGGQVDLAVSDLPFGGDPWDPKHAIHLQRELLLSDRLVERLTITSY
                     LSQPLDYWVELALGCDFADIFEVRGWRRPLRGQFYAPRVEQSTLWFQYRGRDGELLRS
                     VVRFRTSPERVGSDGARWSFTLTRHIPVILEWEIGVESVTSAGGGRRVEEHRAELDRG
                     YDGWQQSCSQWTSDLPGFDFALRRGMDDIGALYIEAEGAPILSAGIPWYSTVFGRDAI
                     IASLETLSLTPQIAIDTLRYLAQRQGEKEDPTTEEEPGKIMHELRRGEMARNREIPHI
                     PYYGTIDATPLWLVLLHETWRWTGDFNLVRELLPHAERALEWIDRYGDRDGDLFVEYA
                     PRAPKGLSNQGWKDSGDGVPFPDGELAQPPIALIEVQGYVYDAKTRMADLFRSLGEPG
                     RSEELVSQAEALRCAVLKSFWLEDTGYFALALDGSKRPVPTLTSNAGHLLWSRLPTPE
                     QAARLAEHLLGPELCSGWGIRTLSAAHRVFNPMSYHNGSIWPHDNALIVLGLAHYGKG
                     RAALPIVESIHQAAMRSDLHRLPELFCGMERRPGIRTIRYPVSCSPQAWAAGAPFMLL
                     QAVLGLFPDAPARVLHVRNPALPGFINELTVSGLQVGESRVSLQFRRHRERTLANLLS
                     LEGEPLQVRIELA"
     gene            complement(4347..4472)
                     /locus_tag="VFB89_10840"
     CDS             complement(4347..4472)
                     /locus_tag="VFB89_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27846.1"
                     /translation="MKEAEIARIAKARRPSPPPLGRLGRPDDIADVIAFIASDDA"
     gene            4541..5836
                     /locus_tag="VFB89_10845"
     CDS             4541..5836
                     /locus_tag="VFB89_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HZM27847.1"
                     /translation="MSTPSRRFGFETRMLHAGHIPDAEVGARAVPIYQTTSFVFDDPE
                     SAAQLFELKQYGNIYTRIANPTTAVFEERLASLENATGAVATASGMAAQFVTFMTLLE
                     PGDEIVASAHLYGGSVTQLTHTLRKLSVEVRFVDPTRLDEWERAISPRTRALYGETIG
                     NPRGSILDLEPLAALAESRRIPLIVDNTFATPWLCRPLDWGATITLHSATKFLGGHGT
                     SIGGVLAESGKFDYSHFSTIAKPSPAYHGLNFYENFGHYGFLMKARAETVRDTGACLS
                     PTNAFLLIQGLETLSVRMERHVANTLAVARFLSEHPAVAWVEYAGLPEHPQHALARKY
                     VCGRPGAVFAFGLDAGNDGREAGARFIRALQIFSHLANVGDARSLVIHPASTTHQQLT
                     DDELVRCGVPPEMIRLSVGLETLEDLLWDLDQALRATSS"
     gene            5797..6339
                     /locus_tag="VFB89_10850"
     CDS             5797..6339
                     /locus_tag="VFB89_10850"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HZM27848.1"
                     /translation="MGPGSGPACDIQLNTQLTEDQRRRFQNVDTIRRVLQQSHTIAIV
                     GLSTDPAKASQFVGTYLQHEGYRIVPVNPKASELLGERAWPDLESIPFPVDLVDVFLP
                     GRLCKAIAEQAIAIRAPAIWFQLRIDALEAAELAERAGLAVVMDRCVKMEHGRWAGSL
                     HWAGMNTEIVTARKRQRGLL"
     gene            complement(6321..6887)
                     /locus_tag="VFB89_10855"
     CDS             complement(6321..6887)
                     /locus_tag="VFB89_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27849.1"
                     /translation="MRYRILAWVGTLVGGTIVLILVSLLVITRSAAKGTDIGSPGATD
                     TLPPAGLPGTTDAQAPKRDTTVGPEAPKHHPNEVIPNQGPLDSSLIDLDRGPRQRAAM
                     DIVLRKGIFYQVKEVKPEIMRAVLGSGFYSEPDKYRNPLVRDLYHAYNDGRPAKEPYR
                     IELWSMEAKLGEYVSDTFFLGPRYSKPR"
     gene            6965..7480
                     /locus_tag="VFB89_10860"
     CDS             6965..7480
                     /locus_tag="VFB89_10860"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HZM27850.1"
                     /translation="MRLPLLLTMVGLMIFASPVPARAQDEKAQEVIKKFKELKPELAE
                     YFKNAQGYAVFPSVGKGAIGVGGAHGNGFVYERGKLIGKSSLTQVTIGLQLGGQAYSE
                     IVFLQDKAAMESFKKEKLKLDAQASAVAVTARAGLDLPYRNGVAIATMVKGGLMYEAS
                     VGGQHFSFNPL"
     gene            complement(7500..9443)
                     /locus_tag="VFB89_10865"
     CDS             complement(7500..9443)
                     /locus_tag="VFB89_10865"
                     /inference="COORDINATES: protein motif:HMM:NF014428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZM27851.1"
                     /translation="MPKRNPLQPLHQTRRWISGGIGLGAALATILASAHSCGLIGDEG
                     TRYSVANLAVSWIGLTPVSDTARSLGDTLRYVATVTDRRGTALVGAAIEWKTDDAAVA
                     MVDSAGFVIARAPGATNLTATVAGKVARARIVVRPKAARFEFGSDSVLRVPEGGHASI
                     TVKPQDARGHPILRSAASLHTGDSALARVEGSDVIGRAAGRTALIAELDGARDSIFLD
                     IVPVPGRVTVVKGADQHAGVESRLGEAIGVRVESRVGRPMAGVVVRFAPEEGAGTARP
                     DTAITNADGLAATTWTTGDRPGPERLHATVAGVDSGAVAVAEVEPSRANTRVAQVEDA
                     PSGLAAGADPVVVGIQVTDSSGRALPGVPVVWKLLDGGRIVGRAARTDSAGEAHAEWW
                     LGPRAGVQRAKAQLGSGRTVPPATVLAASFPGAPAKLTGLTSSSVRGVAGTDLSKPVS
                     VRVTDAAGNGVPGVAVSVSGGGPTTDSLVVSDSTGRADIHWRLGEKVGAQALTVSIDN
                     VAPLRFTAAVAPGAPANLALLDPGGAPPARGGAPVQARVTDVYGNPVPNALVVFSVSD
                     GSVKPARVMSGKDGVAATTWKLGRRQGTQSLTALLQKSGAKDVIEMTAPRASAAPAPH
                     SAPRKTASARPTKSSPLTGKALN"
     gene            complement(9618..10799)
                     /locus_tag="VFB89_10870"
     CDS             complement(9618..10799)
                     /locus_tag="VFB89_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZM27852.1"
                     /translation="MTPHSEHPETTAIRTQVPASPEREHSVPLFLTSSFVFESAEQAR
                     ALFAEEIPGNIYSRYSNPNADEFVRKICQLEGADDGVATASGMAAVFATLAALLKAGD
                     HIVAGRALFGSTHQILTRLLPRWGIEHSYAELTDRESWKGVFRPNTRLVFVETPSNPG
                     LDLVDLRWLAELAHTNRTPLVVDNCFTTPYLQQPINHGADLVIHSATKYLDGQGRTLG
                     GVVVGPQGLIGEIRFFARHTGPALSPFNAWILSKSLETLAVRMDRHCSNALTLASHFE
                     GHTELERVRYPFLPSHPQYDLARRQMCQGGGIVVLVLRGGLERARRFLDAVRLLSHSP
                     NLGDTRSIVTHPTSTTHSKLTEEERLRVGIVPGLIRISVGLEHVDDVVADVEQALAAS
                     R"
     gene            complement(10796..>11313)
                     /locus_tag="VFB89_10875"
     CDS             complement(10796..>11313)
                     /locus_tag="VFB89_10875"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZM27853.1"
                     /translation="VRDQEAVSARDSAAESMSEPAPTLRAVVRRRNGAVHFVAETGNG
                     GVVPIDGAAAIGGQGLGARPMELLLSALGGCAGIDVVGILAKQRQELDDLTVTVEGER
                     GAGEPSVFTRIHVHFAASGQVDEAALRRAVELSMEKYCSVARVVERTAAITYSQAIVP
                     AAAIAPESSTP"
BASE COUNT         2101 a         3683 c         3569 g         1960 t
ORIGIN      
        1 cacaaggcga gcacaatcgc tcttcgtcgg ccaatccggt ccgagaacag cccgaacgcc
       61 acacctccga tgatcgctcc gaccatcgag aacgcggtca ggcctgcgcg ggactgcggg
      121 ccgaagcccc actggcgctc gagaaaggtg ggatacatgt cctgggtgcc gtgagacgca
      181 aaattcatcg acgccatcag caccgtgaga tagagaaaaa gccgccagtg tgagacgatg
      241 ccctgcccca gctcccgcca gtttgcatgc ttggtgcgtt cccagacctc ggactccttg
      301 acccgaaacc gaatgaacag cgccagcaga gccggcaagc cgccgatgaa gaacatggga
      361 cgccagccga agcggggata caccaggtag aagcaggccg ctgcgagcag aaagcccatg
      421 gcgtagccct cctggagcaa accggagaga acaccgcgaa ggcgggccgg ggccttctcc
      481 atggcgagcg acgccccgac cccccactcc cctcccatcc cgatgccgaa cagcgcgcgc
      541 agaaccagga aggtcttgta atcgggcgcc agcccggaga ggacttcgac gacggagtaa
      601 aatacgaggt cgagcatcag cggcagtcgc cgaccgtatc gatccgccag caggccgaag
      661 atgagagcgc cgaccggccg gaaggccagc gtcaccgtga tgctcagcgc aatcgcggcg
      721 tcgctcgtcc cgaaatcgcg ggcaatcgcg gttagggcga aaacgacgag gaagaaatcg
      781 aatgcgtcta gagtccagcc gagaaaagcg gcaaggactg cagcgcgatg ctcaccgctg
      841 gctggagcag gtcggcccag ggcaacctcc gtactcaatg cgctaggcag gcaagtcaca
      901 gaaaggttgc caatgataac cgattcggtc gtgacagggc tccttcaacg cgtgaactcg
      961 gcagcgttat actggcgccg acgaatcact caagcaggcc cgcagtatgc gaatcgcgtt
     1021 gatttccacc ccattcgtga gcgcccctcc gcttgattac ggcggtaccg agctggtcgt
     1081 gtacgaattg gccgaaggat tggtcgagca agggcaccac gtcgctcttt tcgcgaccgg
     1141 cgactcgaag acatcggctg acctccgggc gctgtttccc acagcccagt ggccgccgaa
     1201 caccctgagc gacgtgcatc acctctcgtg ggcgcttcag cagatcgctc agcagcattt
     1261 tgacatcgtg cacgcccact ccgcggccgc gcttgccatc gcgaggctga tatccctgcc
     1321 gccgttggtc tacaccatcc atcacaatcg gaacgaggtg ctttcggcct tctaccctta
     1381 ttttccggag gtgcacttcg tcgcgatatc ggcaaatcag caatcacgcg aagttcccct
     1441 ggagcgctgc actgtaatcc accacgggct tgatccaacg cgattcaagt tgaccgagca
     1501 cccgtccgat tacgtctgtt tcgtgggccg tttctcgccg gaaaaggcac ctcatctggc
     1561 catagaagca gcccagaaag ccgaagttcc tattcgagtc gccggacgga ctcacgcgcc
     1621 ggacgcccac tatgcggcca aggagctgga accgcggctc atgttgcccg aggtggaaga
     1681 ccagggaccg gtggggatga aggagaagtc cgagctgctt actcacgcgc gggccctgct
     1741 ggctccggtg caatgggagg aacccttcgg cctcatcctg gtcgaggcga tgatgtccgg
     1801 gtgccccgtc gtcgcctttc ctcgcggaag cattcccgag ctggtcgatc cgggcgagac
     1861 gggcataatc gtgaacagcc tagaagagat gacggatgca atcaggccgg gaggaccggt
     1921 ggaccgcatc gaccgccgcc gctgtcgaac tcgcgcggtc acccgcttca gtcgccagcg
     1981 catggtgcgc gagtacgagc agctgtacca gagagttctg tgggagacca aaccacacct
     2041 gccgatcacc gcgtgagccg aaccgtagag cccgtcccca cccagatcgg ccctgcacct
     2101 gctttcgagg ggcatcggat ctcaatggag gaagccgtag ggtacggggc tatcgactgt
     2161 actcacatgt caccgccgga cagtactctc atcctcaagc aggaccggct ctttctcgtg
     2221 atgacggtcc gaggcgagat cgcgccgcct ggacactgtt cgctcggatt gtttcacgac
     2281 gacacgcgga tcctgagcca ctacgccttg agggttcgcg gtggcgcccc cgctttcctc
     2341 tctgcagatg caccgcggac gtatggcgga caggtcgatc tcgcagtgtc agaccttccc
     2401 ttcggcggag atccctggga tccgaaacac gcaatccacc ttcagcgcga gctcctgcta
     2461 agcgacaggc tggtggagcg tttgacgatt accagctatc tgagccagcc gttggattac
     2521 tgggtcgagt tggcgctggg ctgcgacttc gccgacatct tcgaagtccg gggatggcga
     2581 cggccgctgc gcggccagtt ctacgcgcct cgcgtcgagc agagcacgct ttggttccag
     2641 taccgggggc gggatggtga gctgctccgg agcgtcgtga gattccgcac gtctcccgag
     2701 cgggtaggct cagacggcgc gcgctggtcg ttcacgctga cccggcacat accggtcata
     2761 ctcgagtggg agattggggt cgagtcggtg acctccgctg gcggagggcg gagagtcgag
     2821 gagcaccgag cggagctaga ccgtgggtac gatgggtggc aacaatcctg cagccaatgg
     2881 accagtgact tgcccgggtt cgactttgcg ctgcgacgtg ggatggacga tatcggcgcg
     2941 ctgtacatcg aggcagaagg cgcacccata ctttccgccg ggataccgtg gtattccaca
     3001 gtgttcgggc gcgacgccat catcgcgtcg ctggaaaccc tgtcgctaac cccccagatc
     3061 gccatcgaca ctctgcgata cctggcgcag cgccagggag aaaaggagga tccgactacc
     3121 gaagaagaac cagggaagat catgcacgag ttgcggcgag gtgagatggc gcgcaatcgc
     3181 gagattcccc acatccccta ttacgggaca atcgacgcca ccccactatg gctggtcctg
     3241 ctgcacgaaa cctggcgctg gaccggcgac ttcaatctgg tgcgcgagct gctccctcac
     3301 gcggagcgcg ccctcgaatg gatcgatcgc tacggcgacc gcgatggcga cctcttcgtc
     3361 gagtatgcgc cgcgtgctcc gaaaggcttg tcgaatcagg gatggaaaga ctcgggcgac
     3421 ggcgtgccgt tcccggacgg agagctagcc cagccaccca tcgcgctcat cgaagtgcag
     3481 ggctacgtgt acgacgccaa gacgcgaatg gcggacctct tccgctcact cggggagccg
     3541 ggtcggtcgg aggaattggt gagccaggcg gaggcccttc gctgcgcggt gctcaaatcg
     3601 ttctggctgg aggacacggg atacttcgcg ctggcgctcg atggcagcaa gcggcccgtg
     3661 ccgactctga cttcgaatgc cggccacctc ttgtggagcc gactcccgac tcccgaacag
     3721 gccgcccgat tagccgagca cctgctcggc cccgagctgt gttccggctg gggaattcga
     3781 accttgagcg cagcccatcg cgtgttcaat ccaatgagct accacaacgg ctccatctgg
     3841 ccccatgaca atgcgctcat cgtgctcggg cttgcccact acggcaaggg tcgcgcagcg
     3901 ctccccatcg tggagtcgat acatcaggca gcaatgcgaa gtgacctgca ccggctaccc
     3961 gagttgtttt gtggaatgga gcgccggccc gggattcgca cgatccgata ccccgtgagc
     4021 tgctcgccgc aagcctgggc agccggcgcc cccttcatgc tgctccaggc ggtgcttggt
     4081 ctcttccccg atgctcccgc gcgggtgctc cacgtgcgca acccggccct gcccggcttc
     4141 atcaacgagc tcactgtgtc cggtttgcag gttggcgaga gtcgggtatc actgcagttc
     4201 cgccgtcacc gggagcgaac cctggccaac ctgctcagcc tcgagggcga acccctgcag
     4261 gtccgtatcg agctggcgta atactgccct gcaactgctc agaggcgaag gcctccgctc
     4321 acgttgatca cctgccccgt gatccatcag gcatcatccg aagcgataaa ggcgatcaca
     4381 tcagcgatgt cgtccggacg gccaagccgg ccgagggggg gcggcgaggg tcggcgagcc
     4441 ttcgcgatcc gggcgatttc tgcttccttc atacgggcag actggcgcta acgactctcc
     4501 ggatcaagcc gatacgccgc gccggctgta aacttctggc atgagcaccc cttcccgccg
     4561 cttcgggttc gagactcgaa tgctccacgc cggccacatc ccggatgcgg aggtgggcgc
     4621 ccgcgcggtt ccgatctatc agaccacctc attcgtgttc gacgaccctg agagcgcggc
     4681 gcagctgttc gagctcaagc agtacggaaa catctacacc cggatcgcca atcccacgac
     4741 ggcagtcttc gaggagcggc tcgcctctct ggagaatgcg accggggcgg tcgcgacggc
     4801 gagcggtatg gcggctcagt ttgtcacctt catgacgctg ctcgagccgg gagacgaaat
     4861 cgtcgcctcg gcccacctct acggcggctc ggtaacgcag ctgacccaca cgctgaggaa
     4921 gctgtcggtc gaggtgcgat tcgtcgatcc gacccggctg gacgagtggg agcgggcgat
     4981 cagcccgcgg acccgcgcac tgtacggcga aacgatcggc aacccacgcg gcagcatcct
     5041 cgatctcgag ccgcttgccg cgctggcgga gagccgtcgg atcccactga tcgttgacaa
     5101 caccttcgcc actccctggc tctgccggcc cctggattgg ggcgccacca tcacacttca
     5161 ctccgctacc aagttcctgg gcggccacgg cactagcatc ggcggcgtgc tggccgagtc
     5221 gggcaagttc gactattccc atttttccac aatcgcgaag ccgtcgccgg cgtatcatgg
     5281 gctcaacttc tacgagaact tcggtcatta cgggttcttg atgaaggctc gggccgaaac
     5341 ggtacgtgac accggggcat gcttgtcccc caccaatgcg tttctcctga tccagggcct
     5401 ggagaccttg tcggttcgga tggagcgaca cgtcgcgaat accttggcgg tcgcccggtt
     5461 cctctccgag cacccggcgg tcgcctgggt cgagtacgcc ggcctgccgg agcacccgca
     5521 gcatgcgctg gcccggaagt atgtgtgcgg ccgaccaggc gcggttttcg cctttgggct
     5581 ggacgccggc aacgatggac gcgaggcggg ggcccgcttc attcgcgcgc ttcagatctt
     5641 ctcgcacctg gccaacgtgg gcgacgcccg cagcctggtc attcaccccg cttcgacgac
     5701 gcaccagcag cttaccgacg acgagttggt ccgctgtggg gtaccgcctg agatgattcg
     5761 cctgtcggtt ggactggaaa ctctggagga cctgctgtgg gacctggatc aggccctgcg
     5821 tgcgacatcc agctgaacac tcagctcacc gaggatcagc gccgtcgctt ccaaaacgtg
     5881 gacaccatcc gccgcgtgct gcagcagagt cacacgattg caatcgtcgg cctgagcacc
     5941 gatcccgcca aagccagtca gttcgtgggg acgtatctcc agcacgaggg ctaccgcatc
     6001 gtcccggtga accccaaggc gagtgagctc ctaggagaac gagcctggcc cgacctcgaa
     6061 agcatcccct ttccggtgga tctggtggac gtgttcctgc ccggtcgact ctgcaaggcc
     6121 atcgccgagc aggcgatcgc cattcgggcg ccggccatct ggtttcagct gcggatcgac
     6181 gccctggaag ccgcggagct ggccgaacga gcaggtcttg ccgtagtcat ggatcgctgt
     6241 gtcaagatgg agcacggcag gtgggcgggc agcctgcact gggcagggat gaataccgag
     6301 atcgtgactg cgcgaaagcg tcagcgtggc ttgctgtaac gcgggcccag gaaaaacgtg
     6361 tccgatacgt actccccaag cttggcttcc atgctccaga gctcgatgcg atatggctcc
     6421 tttgccggtc ggccgtcgtt gtatgcatga taaaggtcgc ggaccagcgg attccgatat
     6481 ttgtccggtt cggaatagaa cccgctgccg agcacggccc gcatgatctc cggcttgacc
     6541 tccttcacct gatagaagat tccttttcga agcacgatat ccatggcggc ccgctgccgg
     6601 ggaccgcggt cgagatcgat gagcgagctg tcgagcggcc cttgattcgg gatgacctca
     6661 ttggggtggt gtttcggagc ctccgggcca accgtcgtat cccgcttggg agcctgggcg
     6721 tcggtggtgc cgggcaaacc ggccggagga agcgtatcgg tcgcgccggg ggagcctatg
     6781 tcagtgccct tggcagccga cctcgtaatc acaagcagag agaccaggat caggacgatc
     6841 gtcccgccga ccagggttcc gacccacgcg agtattctgt atcgcatcgt attgccctct
     6901 gctacattga aacacggaag tcacaccacg tccagccgtt ctcctcatcc ggaagaacct
     6961 acgtatgcgc ctgccgctgc tcctgacaat ggtcggactc atgatcttcg cctcgccggt
     7021 cccggcgcgg gctcaggacg agaaggctca agaagtcatc aaaaagttca aggagctaaa
     7081 gccggagttg gccgaatact tcaagaatgc acagggctac gcagtctttc ccagcgtcgg
     7141 caaaggtgcg atcggcgtcg gcggcgccca cgggaatgga tttgtgtacg aacgtgggaa
     7201 gctgattggc aagagcagtc tgactcaggt gaccatcggc ctccagctcg gcgggcaggc
     7261 ctacagcgag atcgtcttcc tgcaagacaa ggccgccatg gagagcttca agaaggaaaa
     7321 gctgaagctg gacgcgcagg cctcggcggt ggcggtgacc gcccgcgccg gcctcgacct
     7381 cccctaccgg aacggtgtcg ccatcgcgac catggtaaag ggcgggctca tgtacgaggc
     7441 ctcagtcggc gggcagcact tcagcttcaa tccgttgtga ttgtaccgaa ggagcgaggc
     7501 tagttcaaag ccttgccggt cagcggcgag cttttcgtcg ggcgtgccga tgcggtcttg
     7561 cggggagcag aatggggggc gggcgccgca ctcgccctag gcgcagtcat ctcaatgacg
     7621 tccttcgcgc cgctcttctg caggagggcc gtcaggctct gagttccctg ccgacgaccc
     7681 agcttccacg tggtagctgc tacgccatcc ttgcccgaca tcactcgcgc cggtttgacc
     7741 gacccatccg atacactgaa cacgacgaga gcattaggga ccggatttcc atacacgtcc
     7801 gtgacgcgag cttgtacggg cgcgccacca cgcgctggcg gtgcgccgcc cggatcgagc
     7861 aacgcgaggt tggcgggtgc ccccggcgca acagcagccg tgaagcgcaa tggggcgacg
     7921 ttgtctatgg aaaccgtgag tgcctgggct ccgacttttt cgcccagtct ccaatggatg
     7981 tcggcgcggc ccgtcgaatc gctgacaacg agggaatcag tggtgggtcc gccgccggac
     8041 acggaaacgg ccacgccggg cactccattg ccggctgcgt cggttactcg cacggacacc
     8101 ggtttcgaca ggtcagttcc cgccaccccc cgcactgacg atgacgtaag cccggtaagc
     8161 ttggccggcg cgccggggaa gcttgcggcc agaacggttg cagggggaac ggtccgtccc
     8221 gagccgagct gagccttcgc gcgttggacg ccggcccggg gtcctagcca ccactccgcg
     8281 tgagcctctc ctgccgagtc ggttcgcgcc gctcggccga cgatccggcc gccatccagg
     8341 agcttccaca ccaccggcac accaggtaac gcccgcccgg acgaatcggt aacctggatc
     8401 ccgaccacaa ccgggtcagc tccagccgcg agaccactgg gcgcatcctc gacctgggct
     8461 acacgcgtgt tggcgcgact cggctccacc tctgcgacgg cgaccgctcc cgaatcgaca
     8521 ccagcaacgg tcgcgtgcaa gcgctcgggc ccgggacgat cgccggtcgt ccaggtagtc
     8581 gctgccaacc cgtcggcatt ggtaatcgcg gtatcagggc gcgccgtccc ggcgccttcc
     8641 tccggagcga aacgaaccac gacaccggcc atcggacggc caacccgtga ctcgacccgc
     8701 acgccgatcg cctcacccag cctactctcc acgccggcgt gctggtctgc gcccttcacc
     8761 acggtgacgc ggcccggcac tgggacaatg tcgaggaaga tgctgtcgcg tgcgccgtcc
     8821 agctccgcga tcaacgccgt ccgtcccgcc gcccgaccaa tcacatcgga tccctctact
     8881 cgagcgagag cactgtcacc ggtgtgcaga ctcgccgccg agcggagaat gggatggcct
     8941 cgcgcatcct ggggcttgac cgttatcgag gcgtgcccac cttcgggcac gcgaagaacg
     9001 ctgtccgagc cgaattcgaa gcgagcagcc ttcggccgaa ccacgatacg cgctctcgcg
     9061 accttacccg ctaccgtcgc cgtgaggttg gtcgcgccgg gtgcacgggc gatcacgaat
     9121 ccggcgctgt cgaccatcgc gaccgcagcg tcatcggtct tccactcgat ggctgccccg
     9181 accagcgcgg tgcctctccg atccgttacc gtggccacat accgaagcgt gtcccccaag
     9241 gacctcgcgg tgtcgctgac gggcgtcagc ccgatccagc tcaccgccag attcgcgacc
     9301 gagtagcggg tgccctcgtc gccgatcagg ccgcaggagt gagccgaggc gagaatcgtc
     9361 gcaagggcgg cgccgaggcc aatgcccccg ctgatccacc gacgggtttg atggagaggc
     9421 tgcaaaggat tgcgtttggg cacggttgag ggattggaga caggccgtat ccctgcacgg
     9481 atcgaaccag cccggccggg ccgggcaggg gtgctaatgg cttggtttac aacgcctttg
     9541 gatggcctgc caggatgcgt ttcaggcccg gcaccgggca acttgcgacc ccggctggcg
     9601 gaacggctgc cgatcgatca gcgggatgct gcgagcgctt gctctacgtc ggctacgaca
     9661 tcgtccacgt gctcgagccc aaccgagatc cggatgagtc cggggacgat tcccacgcga
     9721 agtcgctctt cttccgtgag cttcgaatgt gtcgttgatg tcgggtgggt gacgatgctg
     9781 cgggtgtcgc ccaggttcgg actgtgtgag agcaaccgta cggcgtcgag aaatcggcgt
     9841 gcgcgctcga gcccgccgcg caggacaagg acgacgatac cgcctccctg acacatctgg
     9901 cggcgggcca gatcgtattg cggatgggaa ggaagaaagg gataacgtac ccgctccagc
     9961 tcggtgtgtc cctcgaaatg actggccagg gtcaaagcgt tgctgcagtg ccggtccatc
    10021 cgcaccgcga gagtttccag gctcttcgac aggatccacg cattgaacgg cgagagcgcc
    10081 ggtccggtgt ggcgcgcgaa gaaacgaatc tcgccgatca gtccctgcgg gccgaccacg
    10141 acgccgccaa gcgtgcgacc ctgcccgtcc aagtatttgg ttgcggagtg aatgacgagg
    10201 tcggcaccgt ggttgatcgg ctgctggagg tagggcgtgg tgaagcagtt gtctactacc
    10261 agcggggtac ggttggtgtg tgccagctca gcgagccacc ggagatctac caggtcgagc
    10321 cccggattgg atggggtttc cacgaacacg agccgtgtgt tgggacggaa cacgcctttc
    10381 caactttcgc gatcggtcaa ctcggcgtag ctgtgctcga tcccccagcg cgggagcaga
    10441 cgggtaagga tctgatgagt ggagccgaat agggcacgtc ccgccacgat gtggtcgccc
    10501 gccttgagca gggccgcgag cgttgcaaag accgccgcca taccagaggc agtcgcgacg
    10561 ccgtcatccg cgccttccaa ctggcagatc ttccggacga actcatcggc gttcgggttg
    10621 gaataccggc tgtagatgtt tccgggaatc tcctctgcga aaagcgcgcg cgcctgttca
    10681 gcgctctcga agacaaagct ggatgtgaga aacaagggca ccgagtgttc gcgctccggc
    10741 gaggccggca cctgagtccg gatcgctgta gtctccgggt gctcggagtg cggcgtcacg
    10801 gcgtggacga ctctggagcg atcgctgctg ctgggacgat cgcctgacta taagtgatcg
    10861 cggcggtccg ctcgaccacg cgcgctaccg agcaatactt ctccatggac agctccactg
    10921 ctcgccgcag ggctgcctca tcgacttgac cgctggcggc gaagtgcacg tgaatccggg
    10981 taaataccga gggctcaccc gccccccgct ctccctccac ggtgaccgtc aggtcatcca
    11041 gctcctggcg ttgcttggcg agaatgccga cgacatcgat cccggcgcag ccgccgaggg
    11101 ccgacagcag cagctccatg ggccgcgcac ccaatccctg cccgccgatg gcggccgcac
    11161 cgtcgatcgg caccactccg ccattgccgg tctcggccac gaagtgcacc gcaccgtttc
    11221 ggcggcgcac gacggcacgg agggtcgggg ccggctcgct catcgactcg gctgcggagt
    11281 cacgcgcaga tacggcttcc tggtcacgaa ccc
//
LOCUS       DASRJQ010000476         5505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2625377, whole genome shotgun sequence.
ACCESSION   DASRJQ010000476 DASRJQ010000000
VERSION     DASRJQ010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5505
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2625377"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..509)
                     /gene="rpsH"
                     /locus_tag="VFB89_10880"
     CDS             complement(114..509)
                     /gene="rpsH"
                     /locus_tag="VFB89_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HZM27854.1"
                     /translation="MSLTDPVADMLTRIRNACTAGHRRVDMPVSKLKSELARLLRDNH
                     YIADFKVLDDGAHGVLRLYLKYHNDEPVIRELRRVSAPGLRRYVKCSEIPRVKNGLGM
                     AIVSTPKGLMTDREARTANLGGELLAVVW"
     gene            complement(521..706)
                     /locus_tag="VFB89_10885"
     CDS             complement(521..706)
                     /locus_tag="VFB89_10885"
                     /inference="COORDINATES: protein motif:HMM:NF005974.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HZM27855.1"
                     /translation="MAKTAWIERAKRTPKFKVRTVRRCQRCGRSRAVLRKFGLCRICF
                     RELALKGEIPGVRKASW"
     gene            complement(787..1341)
                     /gene="rplE"
                     /locus_tag="VFB89_10890"
                     /pseudo
     CDS             complement(787..1341)
                     /gene="rplE"
                     /locus_tag="VFB89_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682374.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
     gene            complement(1503..1883)
                     /gene="rplX"
                     /locus_tag="VFB89_10895"
     CDS             complement(1503..1883)
                     /gene="rplX"
                     /locus_tag="VFB89_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HZM27856.1"
                     /translation="MKPLVYKKAKRIRHAPPVRQKLHITKGDTVQVVSGDDKGKRGRV
                     LRVIPKSGRIVIEGVNVVKRHRRATPTAEGGIVSFAAPIHHSKAMLVDPKTGEPTRIR
                     RRKDKDGTLERIAVKSDQPIPRTR"
     gene            complement(1885..2253)
                     /gene="rplN"
                     /locus_tag="VFB89_10900"
     CDS             complement(1885..2253)
                     /gene="rplN"
                     /locus_tag="VFB89_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HZM27857.1"
                     /translation="MVQQESILRIADNSGARRALVIRVLGGSKRRYAGLGDMVVVAIK
                     DAIPTGQVKKGDVAKAVIVRTAKEVRRKDGSYIRFDENAAVLINDAGEPRATRIFGPV
                     ARELREKRYMKIVSLAPEVL"
     gene            complement(2269..2532)
                     /gene="rpsQ"
                     /locus_tag="VFB89_10905"
     CDS             complement(2269..2532)
                     /gene="rpsQ"
                     /locus_tag="VFB89_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HZM27858.1"
                     /translation="MADTKTRGRRKVRQGVVTSDKMMKTVVVSLNRRFAHPVYGKQVI
                     RTKNVKARNEHDAKVGDTVRIMETRPLAKTVRWRVVEVVERAK"
     gene            complement(2537..2737)
                     /gene="rpmC"
                     /locus_tag="VFB89_10910"
     CDS             complement(2537..2737)
                     /gene="rpmC"
                     /locus_tag="VFB89_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HZM27859.1"
                     /translation="MKPAELREKSTDELEDQLRELTEERFRLRFRSATESIENPMRFR
                     AIRRDIARIKTVLRQRAGQAQG"
     gene            complement(2739..3152)
                     /gene="rplP"
                     /locus_tag="VFB89_10915"
     CDS             complement(2739..3152)
                     /gene="rplP"
                     /locus_tag="VFB89_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HZM27860.1"
                     /translation="MLAPKRIKFRKMFKGRTKGIAQRGNQVSFGEFGLMSLEPGWITS
                     RQIEASRVAMTREMKRGGKVWIRIFPDKPITKKPAETRMGKGKGNPEGWVAVVKPGRV
                     LFELEGISEDLAKKALALASAKLPVKTRFVHREEG"
     gene            complement(3156..3815)
                     /gene="rpsC"
                     /locus_tag="VFB89_10920"
     CDS             complement(3156..3815)
                     /gene="rpsC"
                     /locus_tag="VFB89_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HZM27861.1"
                     /translation="MGQKTHPIGLRLGIVKPWQSRWFARKDMPALLKEDELIRKYLKT
                     RLGHAAISEIHIDRRPGKVTVTVHTGRPGVVIGKRGAEVDKLRDELAQLTGKEAAINV
                     EEIKRPELASQLVGDNIAHQLEQRISFRRAMKRAVQSAMRMGAQGIKIRVSGRLGGAE
                     IARTEGYREGRVPLHTLRADVDYATSTAKTTYGTIGIKVWIFKGEVIEDMAGRTYSTG
                     A"
     gene            complement(3816..4172)
                     /gene="rplV"
                     /locus_tag="VFB89_10925"
     CDS             complement(3816..4172)
                     /gene="rplV"
                     /locus_tag="VFB89_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010896322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HZM27862.1"
                     /translation="MSGRAIQRGVRQSPRKMRLVIDLIRGKDVNEAYAILKFNKRLAA
                     KQIAKTLRSAVANAEQAALKDSERFDPDTLVVTKAIVNGGQPLKRFTAAAQGRATPIR
                     KRTSHVEIHVSAKENE"
     gene            complement(4176..4466)
                     /gene="rpsS"
                     /locus_tag="VFB89_10930"
     CDS             complement(4176..4466)
                     /gene="rpsS"
                     /locus_tag="VFB89_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HZM27863.1"
                     /translation="MARSVKKGPFVQESLLTKVQILNSRNEKRVVKTWSRASTILPEF
                     VGHTFAVHNGNKFVPVYVTENMVGHKLGEFAPTRLFRGHSGKLVADKKAKPE"
     gene            complement(4536..5375)
                     /gene="rplB"
                     /locus_tag="VFB89_10935"
     CDS             complement(4536..5375)
                     /gene="rplB"
                     /locus_tag="VFB89_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HZM27864.1"
                     /translation="MSIRQFRPMTAATRFRSVSGFDEITRDTPEKSLLEPKTRTGGRN
                     NLGHVTARYKGGAHKKMYRRIDFKRDKLGIPGRVTEIEYDPNRSARIALVVYADGEKR
                     YILHPKGLAVGDSVVSGPGADIRIGNAVPLAEVPLGTAVHNVELKIGKGGQLSRTAGA
                     SAQVVAKEGDYVTLRLRSTEMRLVHGRCLATIGEVGNAEHELLSLGKAGKSRWLGRRP
                     RVRGVAKNPVDHPLGGGEGKSSGGRPPTSPWGKREGRKTRHRKNRSNKYIVRGRKRGK
                     ATT"
BASE COUNT          793 a         1941 c         1622 g         1149 t
ORIGIN      
        1 gcgagtgagc tcgcccttcg gccccttcac cagcagcgtg cgaccattga gggacacggt
       61 gacgtccttg ggaatcgcga ccggcttctt tccgatacgg gacatgggcc ctcctaccag
      121 accacggcga gcagctcgcc gccgaggttg gccgtccgcg cttcgcggtc ggtcatgagg
      181 cccttgggtg tggacacgat cgccatgccg agtccgtttt tcaccctggg aatctcactg
      241 catttcacat aacgccggag gcccggggcg gagactcgac gcagctcgcg aatgaccggc
      301 tcgtcgttgt gatacttgag atagagccgg agaacgccgt gtgcgccgtc gtccagcacc
      361 ttgaagtccg cgatgtaatg attgtcgcgc agcagccggg ccagctcgct cttgagcttg
      421 gataccggca tatcgacccg ccggtggccc gccgtgcagg cgttccggat ccgggtcagc
      481 atgtccgcga cgggatctgt caggctcatc cgtagtctcc ctaccagcta gccttgcgca
      541 cgccggggat ctcgcccttc agtgcgagtt cccggaaaca gatgcggcag agtccgaact
      601 tccggagcac ggcccggctc cggccgcacc gctgacagcg gcggacggtg cggaccttga
      661 acttcggcgt gcgttttgcc cgttcaatcc aagcggtttt cgccattgtc cctcagtaac
      721 tgcgtgaacg gtgaacggtg aacgggagtt cgcggtgaac ggagcaaccc gttgaccgtt
      781 taccgttcac gctactctag ccggcgtctc gcccctgaaa ggccatccca attcccgcaa
      841 caaagccagc gccaaatcgt cccgtggggc ggacgtcacg accgtgatgt ccatcccgtg
      901 gatcttttcg accttgtcga agtcgatctc cgggaagatc atctgctcct tgatgccgaa
      961 ggtgtagtta ccccggccgt cgaagctccg gttcggcagc ccgcggaagt cgcgaatgcg
     1021 cggcactgcg agggtgatga accggtcgag aaactcgtac attcgggcgc cccggagcgt
     1081 caccgtaaca cccaccggaa caccggcccg gatgccgaaa ttcgcgatgg ccttcttggc
     1141 tcgggtgatc acggccttct gtcccgtgat cacccccagc tcttccgcag ccgcttcgag
     1201 cgacttcgga ttcttgctcg cgtcgcccat gcccacattc agcacgacct tttccagccc
     1261 gggcacctga tgcgcattgg ccaggccgaa ctccttctgg agccgaggcc ggaccgtctg
     1321 ctcgtagtag acttgaagcc ggggcttgcc ttcacttgcc ttgggcgcgg gatcggtcgc
     1381 gccggccgga gccttgtcct tggcccgccc gccaccgctc ggggccgccc ccttcttggc
     1441 cccttccttc tttggcgcgt ccttcttcgg cgccttctct ttgggcgtct cgtcagccat
     1501 cgtcagcgtg tcctcgggat cggctggtcc gatttgaccg caatccgctc caacgttcca
     1561 tccttgtcct tccggcggcg gatccgagtc ggctcgccgg tctttgggtc gaccagcatc
     1621 gccttcgagt gatgaatcgg tgctgcgaag ctcacgatcc cgccctcagc cgtcggcgtg
     1681 gcccgccgat ggcgcttcac cacgttcacg ccttcgatca cgatccgacc gcttttggga
     1741 atcactcgca gcacccggcc ccgttttccc ttgtcgtcgc ccgacaccac ctgcaccgtg
     1801 tcacccttgg tgatatgcag cttctgccgc accggcggcg catgcctgat gcgcttcgcc
     1861 ttcttgtaga ccagcggctt catgctagag cacctccggc gccagcgaga cgatcttcat
     1921 gtagcgcttc tcgcgcagct cccgtgcgac cggcccgaag atgcgagtcg cccgcggctc
     1981 gcctgcgtcg ttgatcagca ccgccgcatt ctcgtcgaag cggatatagg aaccgtcctt
     2041 gcgacggacc tccttggccg tccgcacgat cactgccttg gccacgtcgc cctttttcac
     2101 ctgaccggtc gggatcgcgt ccttgatggc cacgacgacc atgtcgccca ggccggcata
     2161 gcggcgcttg ctcccgccga ggacgcgaat caccagcgcc cggcgtgctc ccgagttgtc
     2221 ggcgatgcgg aggatggatt cttgttgcac catagattcc tcgaatagct atttcgccct
     2281 ctcgactacc tccaccactc tccaccgcac cgtcttcgcg agcggccggg tctccatgat
     2341 gcgcacggtg tcgccgacct tggcatcgtg ctcgttccgc gccttcacgt tcttcgtgcg
     2401 gatcacctgc ttgccgtaca ccggatgggc gaaccgtcgg ttgagcgata ccacgacggt
     2461 cttcatcatc ttgtcgctgg tgacaacgcc ctgacgcacc ttgcgccgcc cgcgagtctt
     2521 cgtgtccgcc atgcccttac ccttgcgcct ggcctgcgcg ttggcgcagg acggtcttga
     2581 tccgcgcgat gtcgcgccga attgcccgga accgcatcgg attctcgatc gactcggtgg
     2641 ccgagcggaa ccggagccgg aaacgctcct cggtcaactc gcgcaactga tcctcgagtt
     2701 cgtcggtgga cttctctctc agctcagccg gcttcacctc agccctcctc gcggtgcacg
     2761 aagcgggtct tgaccggcag cttggcagac gcgagcgcca gtgccttctt ggccagatct
     2821 tcgctgatgc cctcgagctc gaacaaaacg cgaccgggct tcaccaccgc gacccagccc
     2881 tcgggattgc ccttgccctt ccccatgcgg gtctcggccg gcttctttgt gatcggcttg
     2941 tcggggaaga tccgaatcca caccttgccg ccgcgcttca tttcacgcgt catcgcgacc
     3001 cgactcgcct cgatctggcg actggtgatc catcccggct cgaggctcat cagcccgaat
     3061 tcgccgaagc tcacctggtt gccccgctgc gcgatcccct tggtccgacc cttgaacatc
     3121 ttgcggaact tgatccgctt cggcgccagc atggctcagg cccccgtgct gtaggtccgg
     3181 ccggccatgt cttcgatgac ctcgcccttg aagatccaga ccttgatccc gatcgtgccg
     3241 tacgtggtct ttgcggtgct ggtcgcataa tccacatcgg cccgaagcgt gtgaagcggc
     3301 acccgcccct cgcgataccc ttccgtccgc gcgatctcgg caccgcccag ccgtccggag
     3361 acccggatct tgattccctg cgcgcccatg cgcatggcgc tctgcaccgc gcgcttcatc
     3421 gcgcggcgga atgagatccg ttgctccagc tggtgcgcga tgttgtcgcc caccagctgc
     3481 gaggccagct ccggtcgctt gatctcttcc acgttgattg cggcttcctt gccggtcagc
     3541 tgggcgagct cgtcgcgcag cttgtccacc tcggcgcccc gctttccgat caccacgccc
     3601 ggacgaccgg tgtgcacggt gacggtgacc ttaccgggtc ggcgatcgat gtggatctcc
     3661 gagatcgcgg cgtggccgag tcgggtcttg aggtacttcc ggatcagctc gtcttccttt
     3721 agcagggccg gcatgtcttt gcgggcaaac cagcgggact gccacggctt gacgatgccg
     3781 agccgaaggc cgatcgggtg cgtcttctgg cccatttact cgttctcctt ggccgacacg
     3841 tgaatctcca cgtgactggt gcgcttgcgg atcggggtgg cccggccctg cgccgcggcg
     3901 gtaaagcgct tgagcggctg gccgccgttc acgatcgcct tggtcaccac cagcgtgtct
     3961 ggatcgaacc gctcgctgtc cttgagcgcc gcctgctcgg cattggcgac tgccgagcgg
     4021 agcgtcttcg cgatctgctt ggcggcgagc cgcttgttga acttgagaat ggcgtacgcc
     4081 tcgttcacat ccttgccgcg gatgagatcg atcacgagcc gcatcttgcg gggtgactgc
     4141 cgcacccctc gctggatggc gcgtccgctc atcggttact ccggcttggc cttcttgtcg
     4201 gccaccagct tcccgctgtg tccgcggaag agccgcgtcg gcgcgaattc gccgagcttg
     4261 tggccgacca tgttctcggt cacgtacacc gggacgaact tgttcccgtt gtgcacggcg
     4321 aaggtgtgac ccacgaactc gggcaggatc gtgctcgccc ggctccaggt cttcactacc
     4381 cgcttctcgt tccggctgtt caggatctgg accttggtga gcaaggattc ctgaacaaac
     4441 gggccctttt ttacgctgcg agccatttgg tctccgctat cagctatccg ctatccggct
     4501 atccggctat ccggctatcc ggcacggata gcgtactacg tcgtcgcctt cccgcgcttg
     4561 cgtccccgca cgatgtactt gttcgaccgg ttcttgcggt gccgcgtctt gcggccctcc
     4621 cgcttgcccc agggcgacgt cggcggccgt ccacccgagc tcttgccctc gccgccaccc
     4681 agcggatggt ccacggggtt cttcgcgaca ccccgcaccc gagggcgccg gcccagccag
     4741 cggctcttgc ccgccttccc gagcgacagc agctcgtgct ccgcgttccc cacttcgccg
     4801 atggtcgcga ggcagcgacc gtggaccagg cgcatctcgg ttgaacgaag ccgcaaggtc
     4861 acgtaatcac cttccttcgc gaccacctgc gcgctcgccc ccgcggtgcg tgacagctgg
     4921 ccacccttgc cgatcttgag ctcgacgttg tggacggcgg tgcccagcgg cacctccgcc
     4981 aggggcacag cattgccgat tcgaatgtcg gccccggggc ccgagaccac gctgtcgcct
     5041 accgcgagcc ccttggggtg caggatgtac cgcttctcgc cgtccgcgta caccaccagc
     5101 gcgattcgcg cactccggtt gggatcgtat tcgatctccg tcacccggcc cggaatgccg
     5161 agcttatcgc gcttgaagtc gatccggcga tacatcttct tgtgcgcgcc gcccttgtaa
     5221 cgtgcggtta cgtgtcccag gttgttccgc ccgccggtcc gcgtcttggg ctcgagaagg
     5281 gacttctccg gcgtgtcccg ggtgatctcg tcgaacccgg agaccgagcg gaaacgggtg
     5341 gccgcggtca tcggccgaaa ctgtcggatg ctcatctcag ccctcgaaca cggcgatcga
     5401 gtcgccttcc ttgagggtta cgatcgcctt cttccagcgc gccgtggtgc cgcgagtcct
     5461 tccccgcacc acttcatggc gacgcatctg catggtgcga acggc
//
LOCUS       DASRJQ010000477         7715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_670657, whole genome shotgun sequence.
ACCESSION   DASRJQ010000477 DASRJQ010000000
VERSION     DASRJQ010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670657"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..533)
                     /locus_tag="VFB89_10940"
     CDS             complement(147..533)
                     /locus_tag="VFB89_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27865.1"
                     /translation="MRLAGLLVLPLIVGLAACSDSSLAPLPTDLSGSYNLQSIQISSV
                     TEPSSNGTLQLGTSSYDLALTINGQIQPEDVGTYAIGNPNTWVQTSTTTGVQSFGSFS
                     LSGSTLTITLTSPESTVMVWTKVPQR"
     gene            complement(681..1094)
                     /locus_tag="VFB89_10945"
     CDS             complement(681..1094)
                     /locus_tag="VFB89_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27866.1"
                     /translation="MLSRSGLLKAAALLGAVATLSACSKAAVSTDPEPRRSTRAAKAP
                     STADRLGVPPGKLPPPGLCRVWFPGRPPGHQPKPAPCADVEVGAPAGTWVLYRPSQDK
                     QVVHVRYVDERRDGVIVWIRVFDVKSGAFVRDVTG"
     gene            complement(1248..2195)
                     /locus_tag="VFB89_10950"
     CDS             complement(1248..2195)
                     /locus_tag="VFB89_10950"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HZM27867.1"
                     /translation="MRVAIYGVGGAGGYFGARLARAGADVVWIARGAQLEALTARGLE
                     IESVHGDFTLGPLPASSDPARLGQVDVVILGVKVWQVQEAARAMGPLLESEACVVPLQ
                     NGVEASDDLRSVLGDGPPLGGVAKIISELVAPGHIRHLGGPSSLAFGELDDRESKRVE
                     RLRSALREAGIAIDVPTSIRSAIWEKFLFVASLGGVGAVARAPLGVIRSIPGTRAMLE
                     QAMTEIRDLAAARDVPLPDDVVPQALALVDAQPPAGTTSLQRDLAAGRRSELEWWSGA
                     VVRLGRESGVPTPVHQFMYQSLLPSELRASGRIEYPFQS"
     gene            complement(2196..3794)
                     /locus_tag="VFB89_10955"
     CDS             complement(2196..3794)
                     /locus_tag="VFB89_10955"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27868.1"
                     /translation="MPIIRYLLVVCACLLPVSLTAQKNASPKDTAAKDAKDSTSAKPV
                     ADKPEQWVTRHTITIGGKTISYVATAGTLIVRDSTDAPVASFGYVAYTKPDVKDPATR
                     PLTFAYNGGPGSSSVWLHMGALGPRRVMAADASPTPPPPYRLVDNESSLIDRTDLVMI
                     DPVGTGLSKALGKKKNKDFWGVDPDIESISHFIKQFVTENGRWNSPKYLLGESYGTTR
                     SAGIVDYLQTRENMAFNGVVLVSVALDLEAIFDWPGNDRPYALFVPTYAATSYFHRAL
                     PTPPADLQGFLQEVRAWSLGPYTSALMSGDGLGDSERDAIANKLHQYTGLSVDYIKKA
                     NLRVSEGEYTQELLREHRETIGRLDSRFTGVTFDPLAQNAAYDPQSAAVSGAYTSAFL
                     DYFHRELNVPRDRDYVITARAFEQWDWKHKVPGEPQPLPMVNTGPDLAHALSFNPNLR
                     VLVLNGTYDLATPFLATEYMMSHLNIAPSLKSHITMKYYDAGHMMYLNEPSLVRMKAD
                     VAAFIDGTDRVGQPGDVAKTAVGR"
     gene            complement(3809..5083)
                     /locus_tag="VFB89_10960"
     CDS             complement(3809..5083)
                     /locus_tag="VFB89_10960"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF002772.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HZM27869.1"
                     /translation="MPRPSEIVAVIMGGGAGTRLFPLTKDRAKPAVPLGGKYRLVDIP
                     ISNCINADIRRIFVLTQFNSSSLHRHIQESYRFDQFSPGFVEIMAAQQTPESVAWYQG
                     TADAVRQNLIHLANYPHRLTLILSGDQLYRMNFRALIARHVATGAEVTVATLPVHPDS
                     AGAFGIMQVAPDGRIVRFVEKPKDPAVLASLRVEWAAFGDTEEPVDPDSLLGSMGIYL
                     FERSVLKDALCGDEADFGKHVIPKLLTKHRVFAYLHQGYWEDIGTIRAFYEANLDLCE
                     PLPKFNFYDATAPVFTHARYLPPTKIIKSQIERSLIADGCIINDARIEHSLVGVRSRI
                     DAGTSLRDTLVMGHDFYESPDRVQHGAPPMGIGHGTVIEGTIVDKNARIGAGVRISSE
                     GKPAELDGRNFYVRDGIVIIPKNAVIPDGTII"
     gene            5247..5645
                     /locus_tag="VFB89_10965"
     CDS             5247..5645
                     /locus_tag="VFB89_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27870.1"
                     /translation="MSRTHRLTPAWVLHLMLVAALGACSRNAPPAPEPGTKEANIIVT
                     VQNQNTADVDVFANINGVPQRLGTVTSQQSGNFEINWDQIGPSGRFSVVVSPIGSGGA
                     YRSGALPIGPGQQVAVQVAPVLRNSITQVQ"
     gene            5813..6235
                     /locus_tag="VFB89_10970"
     CDS             5813..6235
                     /locus_tag="VFB89_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27871.1"
                     /translation="MTPPISQGSGRPQPRAWATPLLLAAALLGCSHNSPAPPTPEAGT
                     PGANVLVTVKNLNTSDVTVYANINGVQQRLGTVSSQQTANFQLDWGRIGPSGRVSMVV
                     DQIGSSGGYRSGAVTLRPGAQVTVNVAPSLRNSTTQVY"
     gene            complement(6232..7617)
                     /locus_tag="VFB89_10975"
     CDS             complement(6232..7617)
                     /locus_tag="VFB89_10975"
                     /inference="COORDINATES: protein motif:HMM:NF011649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HZM27872.1"
                     /translation="MYAHTVGSVRYHFPDLKTLLAKATPFRSGDALAGIAAESAEERV
                     AAQMALADLPLAVFLNEAVVPYEKDDVTRLILDTHDNDAFAPVAHLTVGGFREWLLSY
                     AVDSNVLTALSRGLTPEMVAAVSKLMRAQDLILVASRCRVETGFRTTLGLPGRFSTRL
                     QPNHPTDDPQGIAASVLDGLLLGSGDAVIGINPVSDSPRTVAILLGLLDQLRLRWAIP
                     MQACVLAHVTTQLEALREGAPVDLIFQSIAGTEAANRSFGVSLALLNEARTAALELKR
                     GTVGRNVMYFETGQGSALSANAHHGVDQQTLEARAYGVARVHAPLLVNTVVGFIGPEY
                     LYDGKQILRAGLEDHFCGKLLGLPMGCDVCYTNHAEADQDDMDALLTLLATAGCTYIM
                     GVPGADDVMLHYQSTSFHDALYVRRLLGLRPAPEFEDWLQRMGILDAGGRLTGAAERK
                     LLRGQLALEES"
BASE COUNT         1471 a         2413 c         2470 g         1361 t
ORIGIN      
        1 cttcgactcg accaaaagcg gcctctgggt gcgtgcagac accggcacca cagtctggaa
       61 gttggaatcc tggagcgccg atcggatcac cccggctgag agtacgctcg cagtgcggcg
      121 gaaacactag ccagagtgcc actacatcac cgctgcggca ctttcgtcca caccatgacg
      181 gtcgattcgg gtgacgtcaa cgtgatcgta agggtcgatc ccgacagtga gaatgagcca
      241 aacgattgga cgccggtagt ggtggaggtt tgtacccagg tgttcgggtt tccgattgca
      301 taggtgccaa catcctccgg ctggatctgc ccattgatcg tgagcgcgag atcgtacgag
      361 gacgtaccca gctggagtgt gccgttcgag ctcggctccg tcacgctgga gatctgaatc
      421 gattgcaggt tatacgaacc ggaaagatcg gtaggaaggg gagccaacga gctgtcgctg
      481 catgccgcta atccgacgat tagcggtagt acgagcagcc ccgcaagtcg cacgacacct
      541 cctgatgccg acgcgctgtg ttagcgagag cggcgtcagg atagaaaaac gtcgcccggc
      601 gggtatcgcg ccgggcgacc cgtcctgcat cacgtacgaa gggaagtgtc gctgcgttga
      661 gtgtgcgtct cgagaccgac tcagccggtg acgtcgcgaa caaacgctcc actcttcaca
      721 tcgaacacac gaatccagac gatcacaccg tcgcgtcgct catcgacgta gcggacgtgc
      781 actacctgct tgtcctgcga cgggcgatac aagacccaag tcccggcggg agcgccaact
      841 tcgacatcgg cgcagggagc gggcttgggc tggtgcccgg gcggacgtcc cgggaaccac
      901 acgcgacaaa gacctggcgg cggcagtttg cccggaggca ctcccaatcg atccgcggtg
      961 ctcggcgcct tggcggcgcg cgtgctgcga cgtggctcgg ggtcggttga gacggcggcc
     1021 ttggaacaag cactgagcgt ggcgaccgcg cccaggaggg cagcggcctt gagcaagccg
     1081 gagcgcgaca gcatgcaaat ctccgaaggg ggattgagcc ttccgggcgg caagtttcat
     1141 gcggcgccgg gaagtcacag cctggcttgt ggatctgccg cgacgcggtg ccctggctcc
     1201 ctcgcgtggg ctggcgttcc gcccggagag gggcggcttg ccgggggtca gctctggaaa
     1261 gggtactcga tgcggcccga cgcgcggagc tcgctgggca agaggctctg atacatgaat
     1321 tggtgaaccg gcgtgggcac cccggactcg cgacccagcc gcacaacggc gccgctccac
     1381 cactcgagct cggaacggcg tcccgcagcc aggtctcgct gcagcgatgt ggtgccggca
     1441 ggaggctgtg catcgactaa agcgagggct tgcggaacca catcatcagg aaggggcaca
     1501 tctcgcgccg cagcgagatc gcgaatctca gtcattgcct gctccagcat tgcacgggtt
     1561 ccgggaatcg agcgaatcac gcccaaggga gcccgggcca cggcccctac cccgccaagc
     1621 gaagcgacga acaggaactt ttcccagatt gctgaacgaa tagacgtggg aacgtcgatt
     1681 gcgatcccgg cttcacggag tgcggatcgc agccgctcta cccgcttgct ctcccggtcg
     1741 tccagctctc caaaagcgag cgagctcggg cctccgaggt gcctgatgtg ccctggagca
     1801 accagctcgc tgatgatctt ggcaacgccg cccagcggag gaccgtcacc cagcaccgag
     1861 cggaggtcgt cggaagcctc gaccccgttc tgaagtggga ccacgcacgc ctcggattcg
     1921 agcagcgggc ccatcgctcg agccgcctcc tgaacctgcc atactttgac gccgagaatg
     1981 acaacgtcta cctggcccag tcgggcagga tcgctgctgg cagggagcgg tcccagggtg
     2041 aaatcgccgt gcacgctctc gatctcgagc ccgcgcgccg tgagcgcctc cagctgtgcg
     2101 ccccgggcga tccagacgac gtcggccccg gcgcgcgcga gacgggcacc gaaataccct
     2161 cccgccccgc caacaccata gatcgcgacg cgcaatcagc ggccaaccgc ggttttcgcc
     2221 acgtcgccgg gctgacccac gcggtcggtg ccgtcgatga atgcggcgac atcggccttc
     2281 attcgtacca aggagggctc attgaggtac atcatgtggc cggcatcgta atacttcatc
     2341 gtgatgtgtg acttgagcga aggcgcgatg ttgaggtggg acatcatgta ctcggtggcc
     2401 aggaacggcg tagccagatc ataggttccg ttgagcacca gcacgcgcag attgggattg
     2461 aagctgagcg catgagccaa gtccgggccg gtgttcacca tggggagcgg ctggggttcg
     2521 ccgggcacct tgtgcttcca gtcccactgc tcgaacgccc gggcggtaat cacgtagtcg
     2581 cggtccctgg gcacgttgag ctcgcggtgg aaataatcga ggaatgccga ggtgtacgcc
     2641 ccgctgaccg cggctgattg aggatcgtag gccgcattct gggccagcgg gtcgaaggtc
     2701 acgccggtga agcgggaatc gaggcgaccg atcgtctcac ggtgttcccg aagcagctcc
     2761 tgagtgtact ccccttccga cacccgaagg ttcgccttct tgatgtagtc caccgagagc
     2821 ccggtgtact ggtgcagctt gttggcaatc gcatcgcgct cgctgtcgcc gagcccgtcg
     2881 cccgacatca aggcgctggt atagggcccg agcgaccagg cccgcacttc ctggagaaat
     2941 ccctggagat cagccggcgg ggttggcagt gcgcgatgga agtaactggt ggccgcgtag
     3001 gtcggaacga agagcgcata gggacggtcg ttgcccggcc aatcgaagat ggcctcgagg
     3061 tcgagcgcta cagacaccag gactactccg ttgaacgcca tgttctcccg ggtctgcagg
     3121 tagtccacga tgccggctga tcgtgtggtt ccgtagctct cgccgaggag gtacttgggt
     3181 gagttccagc gcccgttctc ggtgacgaac tgcttgatga aatgactgat ggattcgata
     3241 tccgggtcca ctccccaaaa gtccttgttc ttcttcttgc ccaacgcctt gctcagtccg
     3301 gttccgacgg ggtcgatcat gaccaggtcg gtacggtcga tgagactcga ctcgttatcc
     3361 accagtcgat atggcggagg cggagtggga ctcgcgtccg cggccatcac gcgccgcggg
     3421 cccagcgctc ccatgtggag ccacacggac gaggagcctg ggccgccgtt gtacgcgaag
     3481 gtgagcgggc gggtcgccgg gtccttcacg tcgggcttgg tgtacgccac ataaccaaag
     3541 ctcgcgaccg gcgcatcggt tgaatcccgg acgatcagcg tccccgcggt cgcgacgtag
     3601 ctgatcgtct tgccgccaat cgtgatagtg tggcgggtga cccactgctc cggcttgtct
     3661 gcgaccggct tggcgctcgt cgagtccttg gcgtctttgg ccgcagtgtc tttgggagat
     3721 gcgttcttct gcgcggtgag cgaaaccggc agcaaacagg cacagacaac gagcaggtaa
     3781 cgaatgattg gcatggcgat gagggtggtc aaatgatggt tccgtcggga atgacggcat
     3841 tcttgggaat gatgacgatg ccgtcgcgaa cgtagaaatt acggccgtcc agctccgcgg
     3901 gtttcccctc cgagctgatg cgcacgcccg cgccgatgcg tgcgttcttg tcgacgatgg
     3961 tcccctcgat cacggtgccg tggccgattc ccatgggggg agcgccgtgc tggacccggt
     4021 ccggtgattc gtagaaatcg tgtcccatga ccagcgtgtc acgcagtgag gtgcccgcgt
     4081 cgatccgact tctgacgccc acgagcgaat gctcgatccg ggcatcgttg attatgcacc
     4141 catcggcaat cagcgaccgc tcgatttggc tcttgatgat cttggtaggc ggcaggtacc
     4201 gcgcgtgggt aaacaccggc gcggtcgcgt cgtagaagtt gaacttcggg agcggctcgc
     4261 acaggtcgag attggcctcg tagaaggccc ggatggtgcc gatgtcttcc cagtagccct
     4321 gatgcagata ggcaaacacc cggtgctttg tcaggagctt cggaatgaca tgcttcccga
     4381 aatcggcctc gtcgccacac agcgcatcct tgagaaccga tcgctcgaac agataaatcc
     4441 ccatcgatcc cagcaagctg tccggatcca cgggctcctc cgtgtccccg aaggcagccc
     4501 attcgacccg cagggaagcc agcaccgccg gatccttcgg cttctcgacg aaccgcacga
     4561 tccgtccgtc cggcgcaacc tgcatgatgc cgaaggcacc cgcagaatca gggtgcaccg
     4621 gaagcgtcgc gaccgtgacc tcggcgccgg tcgctacgtg cctcgcaatc aatgcccgga
     4681 agttcatgcg atagagttga tcgcccgaca ggatcaaggt gagccggtgg ggatagttgg
     4741 cgaggtggat cagattctga cgcacggcat cagcggtccc ctgataccag gcgacgcttt
     4801 cgggcgtctg ctgcgcagcc atgatctcca cgaaacccgg tgagaactga tcgaagcgat
     4861 agctctcctg gatgtggcgg tgcagcgagc tcgagttgaa ctgcgtgaga acaaagatcc
     4921 ggcggatatc ggcgttgatg cagttgctga tcgggatgtc caccagccgg tacttgcctc
     4981 cgagcggcac ggccggcttg gcgcggtcct tggtgagggg aaacaggcgg gtgcccgccc
     5041 ccccgcccat gatgacggcg acgatttcgg agggtcgggg caaaggaaca gccgaagtca
     5101 cggcggaatc ttacggcggc caacgcggcc gagccagcac gcagagctaa acccaccgac
     5161 ccgtgacagc ggttgcagcg tttggccgca cttgggcata tggtctttag gctacacttt
     5221 aaggcggagt tatgagacaa ccccaaatga gccgaaccca ccgactgacc ccagcctggg
     5281 tattgcatct catgctggtt gcggcacttg gggcctgcag tcgcaacgcc cccccggcgc
     5341 ctgagccggg caccaaggaa gcaaacatca tcgtgacggt ccagaaccag aacacggccg
     5401 acgtcgatgt gttcgccaac atcaacggag tgccccagcg cctgggtacg gttacgtcgc
     5461 agcagagcgg caacttcgag atcaattggg accagatcgg accatcgggg cgcttctcgg
     5521 tggtcgtctc gccgattggc agcggcggag cttatcggag cggcgcactg ccgatagggc
     5581 ccgggcaaca ggtcgcggtc caggtcgcgc cggtgctccg aaactcgatt acgcaggtgc
     5641 aatagcgatc tcgtagaggt gccagggaag cggtttgcga tccgtgctgt tttgagcagg
     5701 cggccgtccg ttccaaaatg ccgcctgcct gagccaaggc tcaaacaaac ggttgctgca
     5761 ggattcgatt tccggcagta gattagccgc gccacttcgc cggagatacg tcatgacccc
     5821 cccgatcagc caaggtagcg gccgtcctca gccgagagcc tgggcaacgc ctctgctcct
     5881 ggccgcagca cttctgggct gcagtcacaa ctcaccggcc ccgccgacgc cagaagccgg
     5941 cacgcccggt gcgaacgtcc tggtcacggt caagaacctg aacacgagcg atgtcaccgt
     6001 atacgccaat atcaatggcg tacagcaaag gctggggacg gtgtcgtcac aacagaccgc
     6061 caatttccag ttggactggg gtcggatcgg accgtccggc cgggtctcga tggtcgtcga
     6121 tcagatcggc agcagcggag gctatcggag cggcgcggta acgctgagac ccggagccca
     6181 ggtcacggtc aacgtggcac catcgctccg gaactccact acgcaggtgt attagctctc
     6241 ttcgagcgca agctgcccac gtagcagctt gcgctccgca gcccccgtaa gccgaccccc
     6301 cgcatcgaga atgcccattc gctggagcca gtcctcgaac tccggcgccg gccggagccc
     6361 gagcagccgc cgcacataca acgcatcgtg gaatgacgtg ctctggtagt ggagcataac
     6421 gtcgtcggca ccgggtaccc ccatgatgta agtacatccg gccgtcgcca gcagcgtcag
     6481 cagcgcatcc atatcgtctt gatccgcctc ggcgtgattg gtgtagcaca cgtcgcaccc
     6541 catcggcagg cccagcagct tcccgcagaa atgatcttcc agcccggctc gcaggatctg
     6601 tttcccgtca tacaggtatt caggaccaat gaagcccacg acggtgttga ccaggagcgg
     6661 cgcgtgtact cgggcgacgc cgtacgcacg agcctccagc gtctgctggt ccactccatg
     6721 gtgcgcgttg gcggataggg cgctcccctg ccccgtctcg aagtacatca cgttccgtcc
     6781 caccgttccc cgcttcagct cgagcgctgc agtccgagcc tcattcagca gcgcgagact
     6841 gacgccgaac gatcgattgg cagcctccgt ccccgcgatc gactgaaata tcaggtccac
     6901 cggcgcccct tcgcgaagtg cctcgagctg cgtcgtgaca tgggcgagca cgcaggcctg
     6961 cataggaatt gcccagcgca gacggagctg gtcgagcagg ccgagcagta tggccaccgt
     7021 gcggggacta tcactcacgg gattgattcc gatcaccgcg tcgccgctcc ctaggagcag
     7081 cccgtccagc acgcttgcgg cgatgccttg aggatcatcg gtgggatgat tgggctggag
     7141 tcgagtcgag aatctgcccg gcaggccgag cgtggtgcga aatccggttt cgacccggca
     7201 gcggctggcg acgaggatga gatcctgggc ccgcatcagc ttcgagacag cggccaccat
     7261 ttcaggtgta agcccgcggc tcaatgccgt gaggacgttg gaatcgacag cgtacgacaa
     7321 gagccactcg cggaaaccac cgacggtcag atgggcaacc ggcgcgaacg cgtcgttgtc
     7381 gtgcgtatcc agaatgaggc gggtgacgtc gtccttttcg tagggaacga cggcttcatt
     7441 cagaaatacg gccaggggaa ggtcggccag ggccatctgc gccgcgaccc gttcctcggc
     7501 cgactccgcc gcaatgcctg ccagtgcgtc gcccgaccgg aacggcgtgg ccttggccag
     7561 cagtgtcttg agatcgggaa agtgatagcg aactgagccg accgtgtggg cgtacatgct
     7621 caagctctgg cagccttacc taccgagtgc ctggcggacc aggcccgcca gccactccgc
     7681 ttcacgcttg tcgcgaattc cgctgcccgc attgg
//
LOCUS       DASRJQ010000478         4523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3905019, whole genome shotgun sequence.
ACCESSION   DASRJQ010000478 DASRJQ010000000
VERSION     DASRJQ010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3905019"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            690..1373
                     /locus_tag="VFB89_10980"
     CDS             690..1373
                     /locus_tag="VFB89_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27873.1"
                     /translation="MRPSRKFLLMLSSALVIGAGPVSAQIEHQAGGYLVGSFPMGDWG
                     EIAGFGMALDGTDIIRKGPEKPLAWRLTSGLLYNFSRTVGVPQGNIGPSDKLDLETKN
                     WSLLFGVGPEFGKREGPVMPFIFGTVGFDTYWTSSTLSGTVGGLPYSAEHGDSRLSFA
                     WAAGAGLRKRVVEGHLAEVSVEYRSGAAHKFVLPSEVTTSGTGVFVDRSSRASDQILL
                     RVGTVMSSD"
     gene            complement(1561..2337)
                     /locus_tag="VFB89_10985"
     CDS             complement(1561..2337)
                     /locus_tag="VFB89_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27874.1"
                     /translation="MSDTAFIFLLLPSLFFGIMLFVEIGRRLGTRRIVEETERERVVM
                     TTVEAAIYAWLGLMVAFTFSGAATRFEARRNLTVQEANAIGTAYLRLNLLPATAQPAL
                     RQKFRQYTETRIAVYRVLPDIGASNAQEAQATALQKEIWTDAVTALKDAQPESSLLLL
                     PALNNMIDITTERAIILKTHTPPIVLGTLLVLSMVCSLLIGNGLSRKPTFSVLLHTIG
                     FAVVFTVTMYVILDLDHPRVGLIRLDYADQALTDVLAGMK"
     gene            complement(2476..2862)
                     /locus_tag="VFB89_10990"
     CDS             complement(2476..2862)
                     /locus_tag="VFB89_10990"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HZM27875.1"
                     /translation="MSVRVIRLGTRRVRGEGTRIGTVRRPPRGVPRSEFSNQNWFDVW
                     FPNLAPSLGTMKLGQGASSASQWARFARRYRAEMATPAARHDLELLAALSHTTQFSVG
                     CYCMDESRCHRSILRELLVQRGAKVE"
     gene            complement(2859..3701)
                     /locus_tag="VFB89_10995"
     CDS             complement(2859..3701)
                     /locus_tag="VFB89_10995"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HZM27876.1"
                     /translation="MSPSNSRCQEFLGLHQAGCFVMPNPWDLGSARVLTSLGFPALAT
                     TSAGFAWSLGQRDNHVSLEEALSHFRAIASGVDVPVNGDFEGGFASDPAGVGTHVAAA
                     AATGLAGLSIEDSTGDPSAPLYELSLAVERIRAARRAIDDSRTEVLLTGRSEGFIVGR
                     PDLDETIRRLQAYAEAGADCLYAPGIRDRSDIVAVVRAVAPKPVNVLVNGDFTTVADL
                     AVAGVRRISVGGALARAAWTGFLEAAREIARRGTFTGLARATPSDQLERHFPESRVPG
                     RDKS"
     gene            complement(3698..4501)
                     /locus_tag="VFB89_11000"
     CDS             complement(3698..4501)
                     /locus_tag="VFB89_11000"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZM27877.1"
                     /translation="MHYVDEGAGEVLLFVHGTPSWSFEWRHLIRALAPRWRCVAPDLI
                     GFGLSERPESFSYDPEAHAAALGEFVERLDCESLSLIVHDYGGPIGLPICLRQPERVR
                     RLVLFNTWMWSFRGDRDMEGKARMAGGKLGLFLYRHANLSLRVITPYAFGDRRKLTPA
                     IHRQYLDRFPDPTSRGRVLWPLARAILGSSSYYEALWAQRERLRGRRALIVWGMRDPA
                     FQPRQLARWKEVLPEARVVELTASGHWPQEEEPDRVVGEMQAFLTGSAS"
BASE COUNT          815 a         1500 c         1451 g          757 t
ORIGIN      
        1 tgggtgctgg cggttccacg gttccaagca cctgacgacc gtcgtccaca tctgctgtcg
       61 ccccgtctgg ttgcctatcc acggcaagtg aaaagctcca tatgcgtccg acgcgatgcc
      121 ggccgtttcg ccaacattga atcggcgacc tccgccgtcc ggcgccggag gacgcggcat
      181 cataccggga gggctgagcc tcgtcggctc catttggctt taggctgcag cgactagggg
      241 ttggcgcggc gatggcgcat caacgcccag agcagcagac ctgcaccgat ggcggcccct
      301 agcaggccga gcagtgcgag catccaagca tccgggctac ctcgatcaac gccccagagt
      361 cccagaatgc cacagagcac accagccgag accaaggcga aaccaacgat cgcgaggaca
      421 atgcgagtga gagcagacgt ggcagtcagc ctcctgttgg gaactgcagc ctaacgtata
      481 gccgacaagc tgcagaccgc gaagccgacc gtcagcctca tcggcgggtt aggctgacgc
      541 acgcgaagta gacaggcttc tatgcatccg cagccaggcc cggttgactc aatcggcgca
      601 ttgcctatca tccggcgccc aggcatccta cccaccccgt acgactccaa ccacctcgca
      661 cacgccagga atcagtccgg aggttgcaga tgagacccag tcgaaagttt ctcctcatgc
      721 tgagctcggc cctcgtcatc ggtgcgggtc cggtttccgc acagatcgag caccaggccg
      781 gtggctatct ggtcggatcg ttcccgatgg gtgactgggg tgagatcgcc ggattcggca
      841 tggctctgga cggcaccgac atcatccgca agggcccgga aaagcctctc gcgtggcggt
      901 tgacctcggg actgctctac aacttctcgc gcaccgtggg cgtgccacag ggcaacatcg
      961 gccccagcga caagctcgat ctcgagacga agaattggag tctgttgttc ggagtgggtc
     1021 cggagttcgg gaaacgagaa ggacccgtga tgccgttcat cttcggaacc gtcggcttcg
     1081 acacctactg gacgtccagc acgctctcgg gcaccgtggg cgggctgcct tattccgccg
     1141 agcatggcga ttcccgcctc tcgtttgcct gggcagccgg cgccgggctg cggaagcggg
     1201 tcgtggaggg ccacctggcc gaggtgagcg ttgagtaccg ctccggggct gctcacaaat
     1261 tcgtgctccc gagcgaagtg acgacgagtg gcacgggcgt gtttgtcgac cggtcaagtc
     1321 gcgcatcgga tcagattctg ctgcgtgtcg gtaccgtcat gtcgtccgac tgaggaactc
     1381 gttctcccct ccccccggtg ggcgacgtcg cgcgtatcgg cgtcgcccgc gtgccacctc
     1441 agcggctggt gaaaccgcgc gcgagccgga cacccgcgca gatccatgga agcattccaa
     1501 tacgcgaagg cttcgagcgc ggaccgcatg gtgacctttc gcatccgatc ccacctgccc
     1561 tcatttcatt ccagcgagca catcggtcag cgcctgatcg gcgtagtcca gccggatcag
     1621 accgacacga ggatgatcga ggtcgagtat gacgtacatc gtgacggtga agacgaccgc
     1681 aaacccgatg gtgtgcagca gcacgctgaa cgtgggtttg cgggacagcc cattgccgat
     1741 cagcagtgag cagaccatcg acagcaccag cagcgtgccc aggacgatcg gcggggtgtg
     1801 ggttttcagg atgatggccc tttccgtcgt gatgtcgatc atgttgttca aggccggcag
     1861 caggaggagc gatgactcgg gttgggcatc cttcagagcc gtgacggcat cggtccagat
     1921 ctccttttgc agcgcggtgg cctgagcctc ctgagcgttc gacgccccga tatccggcag
     1981 cacgcgatag actgcgattc tcgtctccgt gtattgccgg aacttttggc gcaacgccgg
     2041 ctgggctgtg gccgggagca ggttgaggcg caggtacgcc gtgccgatgg cgttggcttc
     2101 ctgcaccgtc agatttcgcc tggcctcgaa ccgcgtcgcc gcgccggaga acgtaaacgc
     2161 gaccatcagc ccgagccagg catagatcgc agcctcgacc gtggtcatga cgacgcgctc
     2221 ccgttcagtc tcttccacaa tgcgtcgagt acccagccgg cggccgatct cgacgaacag
     2281 catgatgccg aagaacaggc ttgggagtag caggaagatg aatgccgtat cactcatgcg
     2341 agatcgtcct cttgtgtgtc agtgagggcg ctgcgtgtcg cggcggcatc gactagttcg
     2401 gctggctacg caccgcatgt ctaccttgtt tcactgtctg aggttccgcg cgtcaaggta
     2461 tccgacccgg cgttcctact cgactttcgc cccgcgctgg accagcagct ccctgagaat
     2521 cgaccggtgg cagcgactct cgtccatgca atagcaacca accgagaatt gagtggtgtg
     2581 ggaaagcgcg gccagaagct cgaggtcgtg tcgcgccgcc ggggtcgcca tttcggcccg
     2641 atatctcctc gcgaatcgag cccactgact cgccgaggac gccccctgcc cgagcttcat
     2701 ggtgccgagg ctgggcgcca ggttcggaaa ccacacgtcg aaccagttct ggttcgagaa
     2761 ctcagacctg ggtactccgc gcggaggacg ccgcaccgtt ccgatccgcg ttccctcccc
     2821 acggacgcgg cgcgttccga gtcgaatcac ccgcacgctc atgacttgtc ccgccccggg
     2881 acccggctct cgggaaagtg gcgttcgagc tggtcggacg gcgtcgcgcg ggccaggccg
     2941 gtgaaagtgc cccgcctggc gatttccctc gctgcctcga ggaaaccggt ccaggcggcg
     3001 cgcgccaagg cgccgcccac gctgatgcgc cgcacacccg ccacggccag atccgccacg
     3061 gtggtgaagt cgccgttcac caggacgttg accggcttcg gcgccaccgc acgaaccacc
     3121 gccacgatgt cggagcggtc acgaatgccc ggcgcgtaga ggcagtcggc tcctgcttcc
     3181 gcgtaggcct gcaggcgccg gatggtttca tccagatccg gccggccgac gatgaaccct
     3241 tcggaacggc cggtgaggag cacctcggtt cggctgtcat cgatggcccg gcgcgcggcg
     3301 cggattcgct ccaccgccag cgacaactcg tacagaggag ccgacggatc gccggtagag
     3361 tcctcgatcg agagccctgc cagaccggtg gcagccgccg cggccacgtg ggttcccacc
     3421 cccgcgggat cgctggcgaa tccgccctcg aagtccccgt tgaccggaac gtccacgccg
     3481 gaggcgatgg cgcgaaagtg gctcagagcc tcttcgagcg agacgtggtt gtcgcgttgg
     3541 cccaatgacc aggcgaagcc ggcgctggtg gtcgccagcg ccggaaaccc caggctggtc
     3601 agcacccggg cactgcccag atcccatgga ttgggcatta cgaagcaacc ggcctggtgg
     3661 agccctagga actcctggca ccgactgttc gacgggctca tgacgccgag ccggtcagga
     3721 aggcctgcat ttcgcccacc acccgatccg gctcctcctc ctgcggccag tggccggacg
     3781 cggtcagctc gacgacacgg gcctcgggca gaacctcctt ccagcgcgcc agctgcctgg
     3841 gctggaacgc agggtcgcgc attccccaca cgatcagtgc ccggcgaccg cgcagccgct
     3901 cgcgctgcgc ccacagcgcc tcgtagtagc tcgaggaccc gagaatggca cgggccaatg
     3961 gccacagcac gcggccacgc gaggtaggat cggggaatcg gtcgaggtac tggcggtgga
     4021 tcgcgggggt cagcttgcgc cggtcaccga aggcgtaggg tgtgatgacg cgcagcgaca
     4081 ggttggcgtg ccggtagaga aacaggccca acttgccgcc ggccatccgg gccttgccct
     4141 ccatgtcgcg gtcaccgcgg aagctccaca tccaggtgtt gaacagcacc agccgtctca
     4201 cgcgctcggg ttgccgcaga cagatcggca ggccgatcgg cccgccatag tcgtgcacga
     4261 tcaagctgag tgactcgcag tcgaggcgct cgacgaattc acccagtgcg gcggcgtgcg
     4321 cctccgggtc ataggagaag ctttccgggc gttcggagag tccgaaaccg atcagatcag
     4381 gcgccacgca tcgccagcgc ggcgccaggg cgcggatcag atggcgccat tcgaaggacc
     4441 agctcggagt gccgtgcacg aacaacagga cctcaccggc tccctcgtcg acgtagtgca
     4501 tccggcccgc ggaaagttcc cgc
//
LOCUS       DASRJQ010000479         3468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2207518, whole genome shotgun sequence.
ACCESSION   DASRJQ010000479 DASRJQ010000000
VERSION     DASRJQ010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2207518"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VFB89_11005"
     CDS             complement(<1..247)
                     /locus_tag="VFB89_11005"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZM27878.1"
                     /translation="MSPASQTASEFGLTRIQQIAITVQDVERATAFYRDVLGMRLLFR
                     APPGLSFFDCGGVRLMLSLPEGAEAPKGNSILYYAAED"
     gene            348..791
                     /locus_tag="VFB89_11010"
     CDS             348..791
                     /locus_tag="VFB89_11010"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZM27879.1"
                     /translation="MPTDRASLRNEHLADRLHSAAIHLLRRLRREDDASGVTAPRLSA
                     LSVIVFGGGPVTLGQLAAAEQVRPPTITRLVNGLEAEGLVVRQPDPADGRVTYIKATP
                     KGQALLRAGRSRRVAALAGQLGGLSAAEREVLEKAATVMERLSSS"
     gene            803..1879
                     /locus_tag="VFB89_11015"
     CDS             803..1879
                     /locus_tag="VFB89_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27880.1"
                     /translation="MNCWTAGVLALFAALTAPYLEGQSSAQLSAASLRGVSVGPDGAV
                     WASGTNGTVLRSTDGGRSWSSRPVPDAASLDLRDVEALSASTAFAMVAGTDTARIYRT
                     TDAGAHWVRQYDDTRKGVFLDGIAFWDALHGLAVGDPIDGHFLVLRTDDGGIRWTQLD
                     AGSSPAAITGEAAFAASGTAVTVGSNGRAWIATGGVTEPGASARVFRSTDYGRTWQVS
                     TTPIPAGSASTGIFSIAFRDARNGVAVGGDYAHPDSRRPNVAITADGGVTWQLADSAG
                     ITDYLSSVAYSGGDRPGLVAVGTRGSFMSRDGGRTWIRHDSVSYNAIVPLRSGFLIAV
                     GDSGRTAVVAGPGAKAVPKSSGAP"
     gene            1969..2361
                     /locus_tag="VFB89_11020"
     CDS             1969..2361
                     /locus_tag="VFB89_11020"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZM27881.1"
                     /translation="MPRILLVESDAPLRHVVARILVQEGHDVHEAADSAAGLELWHKQ
                     GADVVLTDLRKVGTSSIEAILELRTFVASLPIIVMPDGVSDLERLAEAQLIGTVSLLT
                     KPFTLTDLLGAIAAATPSDSRSDSSRIA"
     gene            complement(2406..2885)
                     /locus_tag="VFB89_11025"
     CDS             complement(2406..2885)
                     /locus_tag="VFB89_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27882.1"
                     /translation="MLRSPVVLLGIATFACGGDDSGAPSGPGGPVPSVSQVEGTYRLV
                     AISNQPLPYPNAISTGSIGRITGGSLSLVATATDGGDATLLLNEDLRDPDTGACCTGS
                     TVETLPLGWSLSSSGGLTLEGAQPVRATHDGIPVQTITVNVQSLDNTKPPGLFRFQR"
     gene            3301..3374
                     /locus_tag="VFB89_11030"
     tRNA            3301..3374
                     /locus_tag="VFB89_11030"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3335..3337,aa:Ile,seq:cat)
BASE COUNT          595 a         1072 c         1123 g          678 t
ORIGIN      
        1 tgtcctccgc cgcgtagtac aggatcgagt ttcccttggg agcttcggca ccctccggca
       61 ggctcaacat gagccgcaca ccaccacaat cgaagaacga aaggcccggc ggtgcccgga
      121 acagcagcct catgccgagg acatcgcggt agaacgcagt ggcgcgctcg acatcctgta
      181 ccgtgatggc aatctgctgg atccgtgtga gaccgaactc cgaggcggtt tgagaggcag
      241 gcgacatggg gcctccggat gcggaatcgg ttgcgccggg gtcggcgccc ggctagcatt
      301 tctttagcac aactaattag ccaagctaac tatctccacc atacgccatg ccgaccgatc
      361 gcgcaagtct ccggaacgag caccttgccg accgcctcca ctcggcggcc atccacctgc
      421 tgcgccggtt gcgacgggaa gacgacgcca gcggcgtcac tgcgccccgt ctgtccgcgc
      481 tctcggtcat cgtgtttggc ggaggaccgg tgacattggg ccagctcgca gctgccgagc
      541 aggttcgtcc gccgaccata acgcgcctgg tcaatgggtt ggaggccgag ggactggtag
      601 tacgccagcc ggacccggcc gacggtcggg tcacctacat caaagcgacg cccaaggggc
      661 aggcgttgct gcgtgccggt cgcagtcgac gggtcgcggc tttggcgggc cagttaggcg
      721 gcctgagcgc cgccgagcgg gaggtgctcg agaaagccgc caccgtgatg gagcgcttga
      781 gttcgagttg aaggagctgt ctatgaattg ttggacggca ggtgtcttgg cgctctttgc
      841 tgcactgacc gccccgtatc tcgagggtca atcgtcggcg cagttgtctg ctgcctcgct
      901 gcggggagtt agtgtggggc ccgatggagc cgtctgggcc tccggcacca acggcactgt
      961 gctccgttca acggacggtg gtcgttcctg gtcctcccgg ccggttcccg acgcggcctc
     1021 tctcgacctc cgcgacgtcg aggcactcag cgcgagcact gccttcgcca tggtggcggg
     1081 cactgacacc gctcgcattt accgcacgac ggacgcagga gcccactggg ttcgtcagta
     1141 cgatgacact cgaaagggtg tgttcctgga cggcattgca ttctgggacg cactgcacgg
     1201 cttggcagta ggtgacccca tagacggcca tttcttggtg ctgaggaccg atgacggagg
     1261 catccgatgg actcagcttg acgcaggctc ctccccggca gcgatcaccg gcgaggctgc
     1321 ttttgcggcc agtggaactg ccgtgacagt agggtcgaac gggcgagcct ggatcgcgac
     1381 cgggggagtg accgagcccg gtgcgtcagc acgggtgttt cgatccaccg attatggccg
     1441 cacctggcag gtcagcacga cgcccattcc tgcgggcagc gcgagtaccg gaatattctc
     1501 gatcgcgttt cgcgacgctc gaaatggcgt agcggtgggc ggcgactacg cgcatccgga
     1561 cagccggcga cccaacgttg cgatcacagc cgacggcggg gttacctggc agcttgccga
     1621 tagcgctggg ataaccgatt acttgtcgtc ggtggcatac tccggcgggg acagaccggg
     1681 gcttgttgcg gtcgggaccc ggggcagctt catgtcccgg gacggaggac gcacctggat
     1741 ccgccacgat tctgtctcct acaacgcgat cgttccactc cgttcgggat ttctgattgc
     1801 cgtgggcgac agtggccgta ccgcggttgt ggccggcccc ggcgcgaagg cggtgcccaa
     1861 gtcaagtggg gccccttagg gggattaccc gtgtcgagaa agactctatc ccgggtcggg
     1921 ccggggaggg cttccggtcc cgggttctat gatacatcct tataggccat gcctcgtatc
     1981 ctgctggtcg aaagtgatgc tccccttcgc cacgtcgtcg cccgaatcct cgtccaggag
     2041 gggcacgacg ttcatgaggc tgccgacagt gccgccgggc tcgagctgtg gcacaagcag
     2101 ggcgctgacg tcgttctgac cgacctgcgg aaggtgggga ccagcagtat cgaagcgatt
     2161 ctcgagctac ggacgtttgt cgcgtcgctt ccgattatag ttatgcccga tggtgtgagc
     2221 gacctggagc ggctcgccga ggcccagctc attggaactg tatcgctcct gacgaagccg
     2281 tttaccttga cggatttact gggcgccatc gctgcggcaa caccgtccga ctcccggagc
     2341 gactcgagca gaatagcgta atccccccta caccaccggg ttctcctccc cgggccgcca
     2401 ggttcttacc gctgaaagcg gaagaggcct ggcggcttgg tgttgtcgag ggactgcaca
     2461 ttcactgtga tggtctgcac gggtatgccg tcatgcgttg cccgaaccgg ttgagcgcct
     2521 tcgagtgtta gcccgcccga gctcgacaga ctccatccaa gtggcaatgt ctcgaccgtc
     2581 gatccagtgc agcaggcacc cgtgtccgga tcccggagat cctcgttaag taagagcgtt
     2641 gcgtcgcctc catccgtggc cgtggcgacc agcgaaagtg atcccccggt gattctgccg
     2701 atcgaacctg tgcttatcgc attcgggtag ggcagcggct gattagagat ggcgaccagg
     2761 cgataggtcc cctctacctg ggagaccgag ggtacgggcc cgcctgggcc ggaaggtgcg
     2821 ccactatcgt cgcccccgca cgcgaaagtc gcgatcccaa gcaagaccac tggtgaacgc
     2881 agcatgctta gcctgccttc cgtctgattt cggggccgct gttcctcacg aacaatagct
     2941 cgagaggccc gttgccggtg atcagagggt atcgtatgat gcgggaccgt ccttaggggc
     3001 gtgctcatgc tggcattgct ccttattccc ctcgctcgtc cgatttcccg tccgcctggc
     3061 tctcgaacac gatctttcgt ccgtcgggtg accaatcggc gtcgccattc tctcgccgac
     3121 gcgatgcagc tagatgcact ggctcgaagg cgtcccgact cgagttcgct tgggggctcg
     3181 ccgaaatacg gtcagctcgt gacgcggagc tattttgtag acgcggtgcc tcagaggtgc
     3241 gcggtatatg tactattgag gcttagaagg tgcaggtagg gtgctggttg cagacttcga
     3301 gggcccttag ctcaggtggt tagagcagcg gactcataat ccgtcggtcg gtggttcgaa
     3361 tccaccaggg cccattgtac ttgcgcccat gaaccggaga gcgacgatgt cccccaatgc
     3421 tcaccacgat cttcagggcc tcagggccct ggttaccgga gcgacgtc
//
LOCUS       DASRJQ010000480         2605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3973473, whole genome shotgun sequence.
ACCESSION   DASRJQ010000480 DASRJQ010000000
VERSION     DASRJQ010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3973473"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VFB89_11035"
     CDS             complement(<1..397)
                     /locus_tag="VFB89_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27883.1"
                     /translation="MIAMLQLFFGPLILTGLTLWAAWLAFAAEADAELPRSLAGPANN
                     DAIAVSRQLHVAHLACLVLAGAAAGTTVVWWAWAPLSGLARLVLAVGVVWVVGDLMPR
                     VVAAVAPELTAPVRRAAVRTLGPFRPLLRL"
     gene            complement(394..801)
                     /gene="ybeY"
                     /locus_tag="VFB89_11040"
     CDS             complement(394..801)
                     /gene="ybeY"
                     /locus_tag="VFB89_11040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HZM27884.1"
                     /translation="MSVHGRRLPLPASVVRRAVHTVLRGERRRATVSVTFLGRERMRR
                     LNARYKGHDRPTDVLAFTLRQPTGPDLADVYVCAAVAAREARAHRVTLRQELIRLVVH
                     GTLHALGYDHPDGEERAQSAMWRRQERYVEELA"
     gene            complement(803..>2605)
                     /locus_tag="VFB89_11045"
     CDS             complement(803..>2605)
                     /locus_tag="VFB89_11045"
                     /inference="COORDINATES: protein motif:HMM:NF019318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HZM27885.1"
                     /translation="LGASEAAYLLDEDRRAAVRRALKDFLGGMLSRGVADAATLRAES
                     SRWLVFRRGDAERLVPRDSILSFADLMAEAEAHHPGVSDDAGERSLRQVVASLYRPTV
                     VFDPAVTAYRREQLREGVDSVKYSVQAGERIVTQGQPVTQEVRDKLAGLQRELRRRGE
                     DTVMTRTVLGGALYNALILGAFWLVILLYRRETYEQLREMFFFGVLFGFVVVASGALT
                     HLFPWRPELIPIPFAAILVSMLYDGRTAVFAAMTLAILLGGQWALRESNTVFFGVVGG
                     IAAALGMRFVRSRKHLYFTIAIVAAAYTLAALTMGLMTGWAPDVIITTAITGSMVALS
                     SASFALLTVPLAESATRVTTDLTLLELSDLRRPLLQRLAVEAPGTWAHSLAMANLCEA
                     ACHAIGGNGLLARVGCYYHDIGKLKAPKFFVENQQPGDNPHDLLSPEESARIIRDHVA
                     FGLELADQAGLPKVVRSFIPEHHGTSEINYFLHRARRRSPAVDIDIRDFRYPGPRPQS
                     VETAVAMLADSSEAAVRVLEDRKPESVRRAIEHLVSQKLTAGQLEEAPITLRDLDRVK
                     DVFCRSMAGMYHHRIGYPRASGGIGAEFEPAERS"
BASE COUNT          504 a          877 c          852 g          372 t
ORIGIN      
        1 tgagccggag caacgggcgg aacggtccca gcgtccgtac ggcagcgcgg cgcactggcg
       61 cagttagctc gggggccacg gcggcgacaa ctcgcggcat cagatctccc actacccaga
      121 ccacacccac cgcgagcacc aggcgcgcca gaccagacaa cggtgcccac gcccaccaga
      181 ccacggtggt tccggcggca gcaccggcga gaacgaggca ggcaagatgg gcaacgtgca
      241 actggcggga taccgcgatg gcatcgttgt tggcggggcc cgcaagagat cgaggcaact
      301 cggcatcggc ctcagccgcg aacgccagcc aggcagccca gagggtcaga ccggtgagaa
      361 tcagcggacc gaagaagagc tgcagcatgg cgatcatgcg agctcctcca cgtatctctc
      421 ctgtcgcctc cacatggccg actgggcacg ttcttcaccg tcggggtggt cgtaccccaa
      481 cgcgtgcagc gtgccgtgca ccacgaggcg gatcagctcc tggcgcaggg tcacccgatg
      541 cgcgcgcgcc tcgcgggctg ccactgctgc acagacatac acatcggcca ggtccgggcc
      601 ggtgggctga cggagagtga aagccagtac gtcggtaggc cggtcatgcc ccttgtaccg
      661 ggcattcaac cgtcgcatcc gctcccgacc gagaaacgtc acggacaccg tggcccggcg
      721 gcgctctccg cgcagcaccg tgtgcacggc gcgtcgaacc acagacgcgg gcagcgggag
      781 gcgccggccg tgaaccgaca cgtcagctcc gctcagccgg ctcgaactcc gcaccaatgc
      841 cgccgctcgc gcgggggtag ccgattcgat ggtggtacat cccggccatg gagcgacaga
      901 acacatcctt cacgcggtcg aggtcgcgca gggtgatggg cgcctcttcc aactgtccag
      961 cggtgagctt ctggctgacg agatgctcga tggctcggcg caccgattcc ggcttccggt
     1021 cttccagaac gcgcaccgcc gcttccgacg agtcagccag catcgccacc gcagtctcca
     1081 ccgactgagg ccggggtccg ggataccgga agtcgcgaat gtcgatgtcg accgcagggc
     1141 tccgccggcg ggcacgatgc aggaagtagt tgatctcgct ggtaccgtgg tgctcgggaa
     1201 tgaaggaccg gaccaccttg ggcaaccccg cctgatccgc cagctccaaa ccgaaagcaa
     1261 cgtgatcgcg aatgatcctg gccgactcct ccgggctcaa caggtcgtga gggttgtccc
     1321 ccggctgctg attctccacg aagaactttg gtgccttgag ctttccgatg tcgtggtagt
     1381 agcaaccgac tcgcgcgagc aatccgtttc cgccgatcgc atggcaggcc gcttcacaga
     1441 ggttcgccat tgccaagctg tgagcccagg ttccgggcgc ttcgacggcg agccgctgca
     1501 gcagcggccg gcgaaggtcg gagagctcga gcagcgtaag gtccgtcgtg acacgggtcg
     1561 ccgactcggc cagcggaacg gtcagcagcg cgaatgatgc gctggagagt gcgaccatgg
     1621 atccggtgat cgccgtggta atgatcacat cgggcgccca gccggtcatc agacccatgg
     1681 tgagcgcggc cagggtgtat gctgcggcga cgatcgcgat cgtgaaatag agatgcttgc
     1741 ggctccggac gaaccgcata ccgagcgccg ccgctatgcc tccgaccacg ccgaagaata
     1801 cggtgttgct ctcgcgcagg gcccactgac cgcccagcag gatcgccagc gtcatcgcgg
     1861 cgaacaccgc ggtgcggccg tcatagagca tgctgacgag gatggcggca aacgggatgg
     1921 ggatcagctc aggtcgccaa ggaaataggt gggtcaaggc gcccgacgcg acgacgacaa
     1981 acccgaacag aaccccgaag aagaacatct cccgcagctg ttcgtacgtc tctcgccgat
     2041 agagcaggat cacgagccag aaggcgccga ggatgagcgc attgtacagc gctcccccga
     2101 ggaccgtccg cgtcatgacg gtatcctcgc cgcggcgccg aagctcacgc tgcagacccg
     2161 cgagtttgtc gcggacctcc tgagtcaccg gctgtccctg ggtgacgatg cgctcgccgg
     2221 cctgcaccga gtatttgacg gagtcgaccc cctcacggag ctgctcccgg cgataggcgg
     2281 tcacggccgg atcgaagaca acggtgggac ggtacaggga ggcgacgacc tgacgcaggc
     2341 tccgctcacc ggcgtcatcg ctgacacccg gatgatgggc ctctgcttcc gccatgaggt
     2401 ctgcgaagga gagaatcgaa tcgcgcggaa ccaggcgctc ggcatcgcct cggcggaaca
     2461 ccagccagcg actcgactcc gcgcgcaacg tggccgcgtc agcgacacca cgagagagca
     2521 tcccgcccag aaaatccttc agagcccgcc gcaccgccgc ccggcgatcc tcgtcgagga
     2581 ggtacgcggc ctccgaggcg cccaa
//
LOCUS       DASRJQ010000481         2309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_24245, whole genome shotgun sequence.
ACCESSION   DASRJQ010000481 DASRJQ010000000
VERSION     DASRJQ010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24245"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VFB89_11050"
     CDS             complement(<1..577)
                     /locus_tag="VFB89_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27886.1"
                     /translation="MTTSSPSRTRASDRGAHLPLAIVAAALSLVVFGAGLVLSAGRIR
                     SGPESQALLVITLEGAVFWVAGVTAFRRATRAPAALPFLLQCAAWTVTLALGSLLPFS
                     GLPGPGLFLAYGLGSYVQAPSLVHVALALGWPHREERWKSSILSWYVLQGILFLVALA
                     AALSGQEPLFNLVDAVVRANLLSLAAFLVSLI"
     gene            718..1173
                     /locus_tag="VFB89_11055"
     CDS             718..1173
                     /locus_tag="VFB89_11055"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZM27887.1"
                     /translation="MTETISLDKLPAVFSPLAKAVLDGFSEGVVVFDHGGRILYANEP
                     AREALKDWDLTEGAPDLQPRLTALGGRLRPLRIGAMELGEAMFIPGIEGPTTLAAREK
                     EAIVKTLDAHGWKLAETARQLGISRTTLWRRLKAYGLHRDGRSKWSQAS"
     gene            1155..>2309
                     /locus_tag="VFB89_11060"
     CDS             1155..>2309
                     /locus_tag="VFB89_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27888.1"
                     /translation="MVASLLALFLFVADPASSDTNPPFIDAITRDLIARAMARHRAQD
                     SVVSDYQARIRYRLAAAMGRGRWAEVPVTAVEEQEALVRWALPNDLRIDVVGRRFQTR
                     NPDWVFSSVFYRPWFVPRGVGDSVRIFSDNFPATGALHPLAATGPDWYSYALKDSVTV
                     ATPRGDRIKVYQVDVVPRRTARALVAGRLWIEAESAEVVRFTFRYVGTQLFVRPSRSQ
                     RSKASAHRLNQLATRLISVDADLEYALQEGKFWMPYRQAIAGRVELPMLGNLVVPFRA
                     VTTFSDYELNTEKPIAFTLPLPDTTLPWDSVRVLRKMRRDSIRAERRQLNVAATRHRS
                     WNYADRWPGGRYEIHRPPNDSLARYAAWDDSLSLGSAAEEMAQFRDIDADL"
BASE COUNT          437 a          710 c          754 g          408 t
ORIGIN      
        1 ctattaggct caccaggaac gcagcgaggc tgagcaggtt tgcgcgaacc acggcatcga
       61 cgaggttgaa gagcggctcc tggccgctga gtgcagctgc gagggcaacc aagaacagga
      121 taccctgcag cacataccaa ctcagtatcg aagacttcca gcgttcttcg cgatgcggcc
      181 agcccaaggc cagcgcgacg tgaacgagcg agggcgcctg cacgtaggat cccaagccgt
      241 aggcaaggaa cagacccggt cccggcagac ccgagaacgg gagcagcgag cccagcgcaa
      301 gggtgacggt ccaggcggca cactgcaaca agaaggggag ggccgcggga gctcgggtgg
      361 ctctgcggaa tgcagtcacc cctgccaccc agaagacagc tccctccagg gtaatgacca
      421 ggagggcttg actctcgggg ccggatctga ttcgcccggc gctgagcacc aaaccggccc
      481 cgaagacgac caagctcagc gccgcggcca cgatggcgag ggggaggtgt gcaccgcggt
      541 cggacgcgcg cgtcctgcta ggtgaagacg tcgtcacgtt cgcaattggt tggcgatggc
      601 aggtggctgg tcccgacggt gactgaatgg tagtcagttg ggacgcaggt ggaagccgga
      661 gtggcgcatg gacgtcccgg attgaaactt tcccccttcg aacgcgtatc tcgttgcatg
      721 acagagacta tctcactcga caagctgccg gctgtcttct cgccgctcgc caaagctgtc
      781 ctcgacggat tcagcgaagg ggttgttgtt tttgatcatg gcggtcggat tctctatgcc
      841 aatgagccgg cccgggaagc gctcaaggat tgggatctga ccgaaggtgc ccctgatctt
      901 cagccgcgac tgactgcctt gggcgggcgg ttgcgtcctc ttcggatcgg ggccatggaa
      961 ctgggagagg cgatgttcat tcctgggatc gagggaccca cgacgttggc ggcgcgtgag
     1021 aaggaggcta tcgtcaagac gctggacgct cacggctgga agctggcgga aaccgcccgt
     1081 cagctgggca tcagccggac caccctttgg cgccggctca aggcgtacgg actgcatcga
     1141 gacggccgga gcaaatggtc gcaagcctct tagccctttt tctcttcgta gcagaccccg
     1201 cttcttccga caccaaccct ccatttatcg acgccatcac ccgcgacctc atcgcgcggg
     1261 ccatggcgcg tcatcgggct caggatagcg tcgtcagcga ctatcaagcc cggatccggt
     1321 accgtctcgc cgcggccatg ggtcgtggta gatgggctga ggtcccggtg acggctgtcg
     1381 aggagcagga ggcgttggtc cgctgggcgc tgccgaacga cctacggatc gacgtggtag
     1441 ggcggcggtt ccagacccgc aatccggatt gggtcttttc gagcgtgttc taccggccgt
     1501 ggttcgtgcc ccggggcgtc ggcgactcgg tacggatctt cagcgacaac tttccggcga
     1561 ccggagccct ccatccgctc gcggcaaccg gtcctgactg gtatagctac gccctcaagg
     1621 acagcgtcac ggtcgcaacc cctcgaggcg accggatcaa agtttatcag gttgacgtgg
     1681 taccccggcg aaccgctcga gcgctggtgg cgggccggct ctggatagag gcggagagtg
     1741 ctgaggtagt tcgcttcacc tttcggtacg taggcaccca actgtttgtc cgaccatccc
     1801 gctcgcagcg gagtaaagca tcggcccacc ggctaaacca gctcgctacc cggctcatca
     1861 gcgtagacgc cgatctcgaa tacgcgctcc aggaggggaa gttctggatg ccctatcgac
     1921 aggcaatcgc cggccgagtc gagttgccga tgctgggcaa tctggtcgtc ccgtttcggg
     1981 cggttacgac ctttagcgat tacgagctca acactgaaaa gccgattgcg ttcacgctgc
     2041 cgctgccgga cacgactctg ccctgggatt cggttcgagt gcttcgcaag atgcggcgag
     2101 actcaatccg ggcagaacgg cgtcagctca acgtggcggc gacccgtcac cgatcctgga
     2161 actatgccga tcgatggccg gggggccgtt acgagattca ccggccgccc aatgactccc
     2221 tggcgcgcta cgccgcctgg gacgactcgc tatcgctggg ttcagcagcc gaggagatgg
     2281 cgcagttccg cgacatcgac gcagacctg
//
LOCUS       DASRJQ010000482         2026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3691591, whole genome shotgun sequence.
ACCESSION   DASRJQ010000482 DASRJQ010000000
VERSION     DASRJQ010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3691591"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VFB89_11065"
     CDS             complement(<1..249)
                     /locus_tag="VFB89_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27889.1"
                     /translation="MATIIAWIEQTVTAIPLPVLEVWGGFAYLVGIGLAICAFGRFTF
                     RIGSDWGFGRERQTWNEPAFLAIPLTFVLIVASGYAGSF"
     gene            complement(519..887)
                     /locus_tag="VFB89_11070"
     CDS             complement(519..887)
                     /locus_tag="VFB89_11070"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZM27890.1"
                     /translation="MISIVDDDESVREATKGLVRSLGYNAATFASAEEFLSSDRVHDT
                     SCVIADVQMAGLSGIDLQQQLVAQGLVLPVILITAFPDERTRRRAIDAGAVDYLSKPF
                     SDEQLVSCLDIALRDDASKG"
     gene            complement(1003..1638)
                     /locus_tag="VFB89_11075"
     CDS             complement(1003..1638)
                     /locus_tag="VFB89_11075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZM27891.1"
                     /translation="MKQRESEEPVVFVVDDDASVCDAVKSLLRSVGLKVEVFGSALEF
                     LARKLPNAPSCLVLDVRLPGLGGLDFQAELTKADIRVPIVFITGHGDIPMSVRAMKAG
                     AVEFLTKPFRDQELLEAVQLGLKLDGERRDGESATADLRARFASLTAREKEVIAQVTT
                     GRMNKQIAGDMGVSEITVKVHRGKAMRKMGAKSLADLLKMADTLRRSGHQL"
     gene            complement(1809..>2026)
                     /locus_tag="VFB89_11080"
     CDS             complement(1809..>2026)
                     /locus_tag="VFB89_11080"
                     /inference="COORDINATES: protein motif:HMM:NF013589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HZM27892.1"
                     /translation="LLYRLFHEEGVRVVPATGLAEACRCSRERIDMYLQRFDAAELAE
                     LREEDGGITVTCEFCSRTYRFAPGEIA"
BASE COUNT          379 a          670 c          620 g          357 t
ORIGIN      
        1 gaaggaccct gcgtagccgc tggcgacgat caggacgaag gtcagcggga tggcgagaaa
       61 cgcaggctcg ttccaggtct gccgctcccg gccgaacccc caatcgctgc cgatgcgaaa
      121 ggtgaagcgg ccgaacgcac aaatcgcaag gccgatgccg acgagatacg cgaacccccc
      181 ccaaacttcc agcacgggaa gggggatcgc cgtgacggtc tgctcgatcc acgcaatgat
      241 cgtcgccatg tgtgcccgta tctggcgggc cgtcgccgga tgctctgcaa cccgatcggt
      301 ggaggctcag gagatcggca ggagcaaagc tggcaaggcc ggatgttgtc gctgataaca
      361 gtgaacccgc gtcggataat gcgagccgtg agggtatcca gtctacgagc atgcttgcaa
      421 gtcgttccct gaaggacacg gcaggcgcgt ttgcccgttt tcgccggagc agcgatggca
      481 cgctcaacac tgccggacgg caggcgcgta agcggacctt atcccttcga ggcgtcgtcc
      541 ctcagggcga tatcgaggca cgacaccaat tgctcgtcgc tgaagggctt gctcaagtag
      601 tcgaccgctc cagcgtcgat agcgcgcctc ctggtccgct cgtccggaaa agcggtgatg
      661 aggatgaccg gcagcaccag accctgggca accagctgct gctgcaggtc gatgccgctc
      721 aaccccgcca tctgaacatc ggcgatcacg cacgaggtgt cgtgcacgcg gtcggagctc
      781 aggaactcct cggcggaggc gaacgtcgct gcgttgtatc cgagcgacct cacaagtccc
      841 ttggttgctt cgcgcaccga ctcgtcatcg tcgacgatcg atatcatgtg agctttcgtg
      901 cgaatggtcg tgtccgctcc aagtgcacgg tgtcgtgacg cttgtcatcg aaacccccgt
      961 agatatgcgc cacccagccg ttccgcggac ctatacttag gtttagagct gatggccgga
     1021 gcggcgcagc gtatcggcca ttttcaacag atcggcgagc gatttggcgc ccatcttccg
     1081 catcgccttc ccgcgatgaa ccttcaccgt aatctcgctg acccccatgt ccccggcgat
     1141 ctgcttgttc atgcgcccgg tggtgacctg ggcaatcacc tccttctcgc gcgccgtcag
     1201 cgaggcaaag cgggcccgca gatcggccgt cgcactctcg ccgtcgcgtc tctcgccgtc
     1261 gagcttcagt cccagttgaa cggcctcgag cagctcctgg tcgcggaacg gtttggtgag
     1321 gaactcgacg gctccagcct tcatcgcgcg aaccgacatc ggaatatcgc cgtggccggt
     1381 gatgaatacg atcggaactc gaatgtcggc tttggtcagc tccgcctgga aatcgagccc
     1441 gcccagcccg ggcagccgca catcgagcac caggcaactc ggcgcgttcg ggagctttcg
     1501 cgcgaggaat tcgagggccg agccgaatac ctcgaccttc aatcccaccg agcggaggag
     1561 gctcttgacc gcatcgcaca cagacgcgtc gtcatcgacg acgaacacga ccggctcctc
     1621 ggactcccgt tgcttcatcc cggctgctcg cgagctggct ggtctcgtgt atggaaacgc
     1681 acacagtatt gggatcactc taacgcccaa tcgcggatcg gccaatcgag gccgcgccct
     1741 ctcgccggct ccggccaggt ggccgtcata ggctgaaatt cttcggaaaa acccgcccgg
     1801 gtgcgcgctt atgcgatctc gcccggggcg aagcggtagg tgcgcgagca gaactcgcag
     1861 gtgacggtga tgccgccgtc ctcctcgcgc aactcggcga gctcagctgc atcgaagcgc
     1921 tgcaggtaca tgtcgatgcg ctctcgcgag cagcggcagg cctcggcgag ccccgtcgcc
     1981 ggcaccaccc gcacgccctc ctcgtgaaac agccggtaga gcagcc
//
LOCUS       DASRJQ010000483         2691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3671842, whole genome shotgun sequence.
ACCESSION   DASRJQ010000483 DASRJQ010000000
VERSION     DASRJQ010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3671842"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="VFB89_11085"
     CDS             complement(<1..936)
                     /locus_tag="VFB89_11085"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZM27893.1"
                     /translation="MAVAAYTGAMVGRATEREVLNRLLAAAARGSGATVLVEGEAGIG
                     KTRLVQSLVEAARTRDVRTIVGAARPFEATRPFGAVSEALQLRRGSSDARRAGIARLL
                     DGEGEQRAAPARPDTRYRVVEEIIDLVEVQSRDVPLLLALEDLHWADDSTVLVVRTLL
                     DRITYLPVLLVGTLRPSPRSADLDLLLADAAAAGATLLQLAPLSLDDVDDLVRAEIGQ
                     PPGPGLVEVVSRAAGNPLWVVEILRSLSAEGLLQVRSGGLAVTTATLPDSFRQLVLRR
                     LRYLPDATLRTLRVAAVLGDTFSLVDLATVSGRQAW"
     gene            complement(1195..1356)
                     /locus_tag="VFB89_11090"
     CDS             complement(1195..1356)
                     /locus_tag="VFB89_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27894.1"
                     /translation="MSVVPAPPDANRRILNRHVAHSLLQFPGEALDLDQLDNELIRLG
                     DRSDCQQPL"
     gene            complement(2060..2482)
                     /locus_tag="VFB89_11095"
     CDS             complement(2060..2482)
                     /locus_tag="VFB89_11095"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HZM27895.1"
                     /translation="MAEPILGSDLGSENRALTFSLRGALGHDEEMTVIKINAITVPAD
                     SGDELAHRFAARAGAVDDADGFEGFELLKPTDGRERWLVVTRWRDEESFQAWLNSDSF
                     AHGHRSAAESRGQGASTPIATHSEVWSYVRAGGSSTSG"
BASE COUNT          501 a          891 c          848 g          451 t
ORIGIN      
        1 ccaggcttgc cggccgctca ccgtggccag gtcgaccagc gagaacgtgt cgcccaggac
       61 agcggcgacc cgcagcgttc gaagcgtcgc gtccggcaga taccgcagcc gccggagcac
      121 gagctggcgg aaagagtcag gcagcgtggc cgtcgtgacg gcgaggccgc cagacctgac
      181 ctgaaggagc ccctccgccg aaagagagcg gaggatctca accacccaca gtgggtttcc
      241 agccgctcgg ctcaccactt cgaccagccc cggtcctggg ggctgaccga tctcggcgcg
      301 gacaaggtca tcgacgtcgt cgagtgagag tggcgcgagc tgcagcaaag tggcgcccgc
      361 cgccgctgca tctgcgagca gcagatcgag gtcggctgag cgtggcgacg gccgcagcgt
      421 accgacgagc aagacgggta ggtacgtgat ccgatccagc agcgttcgga cgacaaggac
      481 cgtcgagtcg tcagcccagt gcagatcctc gagcgccagt aacagcggta catcccggga
      541 ctgcacttca acgagatcga tgatctcttc caccacgcgg tagcgcgtgt ccggcctcgc
      601 cggcgcggcg cgctgttctc cctcgccgtc cagcagccgc gcgatccccg ctcgccgagc
      661 gtcggacgac ccacgcctaa gttgaagcgc ctccgatacg gcgccgaacg gtcgggtcgc
      721 ctcaaacggt cgagcagcgc cgacaattgt gcggacatca cgcgtgcgcg cggcctcgac
      781 cagactctgt acgagccgag tcttgccgat cccggcctca ccctcgacca gcacagtggc
      841 gcccgagccg cgggccgccg cggccaggag gcgattgaga acctcacgct cggttgcgcg
      901 gccgaccatc gcgcccgtat aggccgccac cgccatggct cgagcgtacg gctcgtcatt
      961 tcccgggctg gagccgctcc agatcggagg gccgcctgtg gaatcaagcg cggcgatcgt
     1021 ggggcacccc ttgatccatc tgatccatca tcggatggat tggggttcgg tggaggccgg
     1081 ctgagactgt cacccgaagg cgggtcgctg aactcgagag ggtcgcggca gatcgagatc
     1141 aactcagcga ccacggggaa agtcaggcct gcggacagct cactgtcgag agactcagag
     1201 cggttgctgg cagtcggatc ggtcacccag tcggatcagc tcgttatcga gctggtcgag
     1261 gtcgagggcc tcaccaggaa actgcagcaa cgaatgcgcg acgtggcggt tcagtatgcg
     1321 ccgattcgca tcaggcgggg ctggaacgac gctcaacgag gcccgctccg ccactttgca
     1381 agtaaggggc tgagagtgga gcctggcctc tatcagaaat cgaacatcgc cgttcggcgg
     1441 gccacagttg ggccacagca ggcggcgata agcggccagc tgcggtcgcc cagggtggcc
     1501 ccggccgagt ttgtttcacg gtgcacgcgg gcgcgcggat tacttccaag ctggtcatgc
     1561 gagttcgatt ctcgtcaccc gctccaatgt cataacccct agccagaagc tgtttcacgg
     1621 ctagcaatct agcgtggcgg tgcggcatta gatcgcggca ggctcgtcat ctccggcccg
     1681 attattcggt gtgcaggcgc agatttgccc tggtcagtaa gccattcgag gtcggacttc
     1741 ccaccttctt aggtgatcac cagcctgatt cgcgggccac acacgggcca caaccgagca
     1801 tgacggacag agctgaggcc tccgggtagt cagtctgact gccgccgaca ctgccgtccc
     1861 gtcgtctgat ctcaccgcga tcatcatgtg ctcgcagctc cgtatctgct gctgcggtat
     1921 ttcgtctccc gggctgatac ttcctggcag agcggcgggc gggcgagaaa gttggcgccg
     1981 tctcaacact gcgaggaaga gcgccagcgt tgggattggg attagtcgga gatatcgctg
     2041 tctcagggct gatcgtcggt cagccggaag tggagctgcc acctgcgcgt acgtaagacc
     2101 agacctcgct atgggtggcg atcggtgtcg acgcgccttg gccgcgactc tcggctgctg
     2161 accggtgacc gtgggcgaag gaatccgagt tgagccaagc ctggaacgac tcctcatcgc
     2221 gccagcgcgt gacgactagc caccgctccc gcccatcagt gggcttgagc aactcgaagc
     2281 cctcaaatcc atcggcgtcg tcgacggccc cagctcgcgc agcgaagcgg tgtgccagct
     2341 catcccccga atcggccggc acggtaatcg cgttgatctt gatgacggtc atctcctcat
     2401 cgtgccccaa ggcgccgcgc aacgagaaag tgagggcacg attctccgag cccaggtcac
     2461 ttccgagaat gggttctgcc aagctgaacc aaggaagctg atcaatacgc cagatcagat
     2521 cccgtctacc tgggcgacct cctcgaagac acaactcccg cccgggatca caccagcagc
     2581 gcctcccgct tcttcaacac cctgccaacc ccgcctcaaa cccccttacg tgtgcgcaag
     2641 tgcaccgtcg tcactccgcg actacagtcg cttattcggt tccggatcgc c
//
LOCUS       DASRJQ010000484         2822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2588626, whole genome shotgun sequence.
ACCESSION   DASRJQ010000484 DASRJQ010000000
VERSION     DASRJQ010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2588626"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..>2822
                     /locus_tag="VFB89_11100"
     CDS             128..>2822
                     /locus_tag="VFB89_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27896.1"
                     /translation="MTALPLALVVGHWQLRTVETDWDAKRAATVESASADLKKSLKRA
                     YRTVQEVAEAAIQAADADRETAFRRLDRALPIWGPEVGIAVLDPTTDVPWAWAGHHRL
                     APTANGDSIAVRTTGYYVVLEARRHSRGGRVVVASELVWAHASVPERDRSLAEILRRR
                     TDVSLAFYPPGVAPNNRDVFDYSEPTTRGRRLLFSVLPVPPEQGVARELVFERASRMV
                     VWLVLLTMVLAFFLAPRPLERYLILAFGIWLSARAPIGAALGARALFSPATFFRPLLG
                     PLSSSAGVLALTGFLVTLAGVWLWRRRAERRWPGLAAGAALLVASPSLVIALARGITP
                     PARGAAPGLWLSWELTLLFAASAPIVLASGLFRGPTQTTSRWRIRAGVAFALLVSMVG
                     VFGWSPEGWPDWYPFLWVPALLLVALPAPRSATILGIGLVAGSSAALATWGAELAGRM
                     QVAQRDVARLGEEPDPLAVPLLEGFADVIRKGPAPSTASEMYALWGESQLGDQAYPAH
                     LGLWTRDGNLKEELPLDSLDLPLPLLSSMVRGLPASQFERVIALRRTPGVHYVLLVRT
                     GASAVMTVGVGPRSRLVPPGRLGRLLDPERRAATPYRLALSPPGPAPAADDSLRWRRE
                     GWALRAERELALPAAPREIRAEIDLRGPLPIFVRGVLVVLFDTGVLALVWLLAEYVAG
                     VPPPPPRWRSLAQSFRFRLAVTMAAFFILPAVGFSIWGFVRLAAEADRSRDLLITQTL
                     RDAQLGAPGLERARGPLVEDQLRDLSRRIDADLALYRGGALVASSAPVLQDLGAVGQF
                     MDPSAFRSLALRGQLELTRDGPMPELGERVGFRVLQPGTPATLGVLATPQVADDSSLG
                     ARQLDLALVLLLATIIGVVAALAGARRAALALSRPVSELRRS"
BASE COUNT          331 a          965 c          999 g          527 t
ORIGIN      
        1 tatggccgtc gagtggctgc ggcgtccatc cttgggctgg gtggccgctg cgtgtgtgct
       61 ggctgctgtg gccgcgcgcg ctttgagccc ctggtccaat tggcggcggc tcgcgctcat
      121 ggtcgccttg actgcgctgc cgcttgcctt ggtggtcggc cactggcagc tgcgcacggt
      181 cgaaaccgat tgggatgcca agcgtgccgc cacggtcgag tcggcatcag ccgatttgaa
      241 gaagagcctc aaacgggcct atcgaacggt tcaggaggtc gccgaggccg ccattcaggc
      301 ggcggacgcc gatcgcgaaa cggcattccg gcggctggat cgggcactgc ccatctgggg
      361 cccggaagtc ggcattgctg tgctcgatcc taccacggac gtcccctggg cgtgggccgg
      421 ccaccaccgc cttgctccaa cggcgaacgg cgattcgatc gcggtgcgga ccaccggata
      481 ttacgtagtg ctcgaggccc ggcgccactc gcgtggcggc cgggtcgtgg tggcgtcgga
      541 gctggtgtgg gcccacgcgt cggttccgga gcgcgataga agtctggccg agattcttcg
      601 gcgccgaacc gatgtgtctc tcgcgttcta tccgccggga gtcgcgccca acaaccgtga
      661 cgtgttcgac tactcggagc cgaccacgcg cgggcgacga ctgctgttca gcgtgcttcc
      721 cgtgccgccg gagcaaggcg tcgcccggga actcgtgttc gagcgcgcca gtcggatggt
      781 cgtgtggctg gtgctgctta ccatggtcct ggcctttttc ctggccccgc gtccactcga
      841 gcgatatctc atcctcgcgt tcggcatctg gctgtccgcc cgcgcgccga tcggcgcggc
      901 actcggggcg cgggcgttgt tctctccggc gaccttcttc cgcccactcc tcggtccgct
      961 ctcgagctcc gcgggtgttc tcgcgctgac cggcttcctt gtcacactgg ccggcgtgtg
     1021 gctctggcga cgacgagcgg agcgccgctg gccgggcctc gcagcagggg cagctctcct
     1081 ggtggcctcg ccatcgctcg tcattgcgct cgcacgcggc atcacgccgc ctgcgcgcgg
     1141 agcggccccc gggctctggt tgagctggga gctgacgctc ttgttcgcgg cgtcggcgcc
     1201 gatcgtgctt gcgagcgggc tcttccgagg tccgacgcag accacgtccc gctggcggat
     1261 tcgcgccgga gtcgccttcg cgctgctcgt gtcgatggta ggagtgttcg gctggagccc
     1321 agagggctgg cctgactggt acccgttcct ctgggtgccc gccttactgc tggttgcgct
     1381 cccggccccg cgctcggcga cgattctcgg aatcggactc gtagccggaa gttctgccgc
     1441 gctcgcgacc tggggagccg agctcgccgg gcgaatgcag gtggctcagc gcgacgtcgc
     1501 ccggctgggc gaggagcctg atccactcgc tgttcctctc ctggaaggct tcgccgatgt
     1561 gattcgaaaa ggccccgcgc cgagcaccgc gtcggagatg tacgcgctgt ggggcgaatc
     1621 gcagctcggc gaccaggcct atcccgccca tctcgggctt tggacccggg acggcaatct
     1681 gaaggaggag ctcccgctcg actcgctcga cctgccgctg ccgctgctgt cttcgatggt
     1741 ccgcggactt cccgccagtc agtttgaacg ggtgattgcg ctgcggcgaa cccccggcgt
     1801 ccactacgtg ctgctggtgc gcaccggggc ttccgccgtg atgaccgtgg gggtcggccc
     1861 tcggtcgcgt ctggttcctc caggtcgcct ggggcgcttg ttggaccccg agcgacgagc
     1921 cgcgacgccc taccggctcg cgctctcccc accaggaccg gcgcctgccg ccgacgactc
     1981 gctccgctgg cgacgggagg gatgggcgct ccgggcggag cgcgagctgg cgttgccggc
     2041 ggcaccgaga gagatccggg ccgaaataga cctgcgtggt ccgctcccga ttttcgtgcg
     2101 cggggtcctg gtcgtgctgt tcgacactgg cgttctcgcg ctggtctggc tgctggccga
     2161 atacgtggcc ggcgtgccgc cgccgccgcc gcgctggcga agcctggcgc aatcgttccg
     2221 gttccggctc gcggtaacga tggcggcctt cttcatcctt ccggccgtcg gcttttcgat
     2281 ctggggcttc gtgcgacttg cggcggaggc ggaccgaagt cgtgatctcc tcatcaccca
     2341 gacgctgcgg gatgcgcagc tgggagcgcc aggtctcgag cgcgcacgcg gtccgctggt
     2401 agaggaccag ctgcgggact tgagccggcg gatcgatgcc gacctggcgc tctaccgggg
     2461 tggagccctg gtcgccagca gcgcgccggt gcttcaggac ctcggcgccg tgggccaatt
     2521 catggacccg tcggccttcc ggagcttggc actgcgagga cagctggagc tgactcgtga
     2581 tggcccgatg cccgagttgg gcgaacgggt cgggttccgt gtccttcagc caggaacccc
     2641 ggcaacgctg ggggtgctgg ccacgccgca ggtggccgac gactccagtc tgggtgcccg
     2701 gcagcttgat ctggcgctgg tcctcctcct cgcaaccatc atcggcgtgg ttgccgcgct
     2761 ggccggcgcc cgacgggcag cgctcgcact gtcacggccc gtgtcggagc tccggcgctc
     2821 cg
//
LOCUS       DASRJQ010000485         2246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_598890, whole genome shotgun sequence.
ACCESSION   DASRJQ010000485 DASRJQ010000000
VERSION     DASRJQ010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598890"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1736
                     /locus_tag="VFB89_11105"
     CDS             <1..1736
                     /locus_tag="VFB89_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HZM27897.1"
                     /translation="LISLAISVAFLFSVAVTLGYPGAPLWEELSSLVTIMLLGHWIEM
                     RSISQAQGALGELAKLLPAAVVRVRGTDTEEIPISELREGDVVLVRPGASVPADGLIQ
                     QGESALNESMITGESSPVDKKPGDRVIAGTINGSGSLHVEVTGTGERTALAGIMRLVA
                     QAQTSRSRAQALADRAAFWLTVIALGVGVMTLVAWLAVGAGAAFAVERLVTVLVIACP
                     HALGLAIPLVIAISTTLGAGAGLLVRDRRGLEEARNLTTVVFDKTGTLTRGEFGVVAI
                     RTVQGLAETEALRLAAAVERDSEHTIAQGMLRSARERNLSVPPAQDFQALPGLGVQAR
                     VDGRTLLVGGPNLLRKRSIAVPVELQRAVDEAAAQGRTAVYLVEDAGGSRATVLATLV
                     VADVVREESKTAVRELHDQRIKVVMLTGDARPVAQAVAQELGIDNVFAEVLPEQKVGV
                     IEQLRSRGERVAMVGDGVNDAPALLTADVGVAIGAGTDVAVEAGDIVLVRSDPRDVSR
                     IVRLSRASYRKMVQNLWWATGYNVVAIPLAAGVLARYGILLSPAIGAVLMSLSTVIVA
                     VNAQLLRRARI"
     gene            complement(1767..>2246)
                     /locus_tag="VFB89_11110"
     CDS             complement(1767..>2246)
                     /locus_tag="VFB89_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27898.1"
                     /translation="ILLDAGRTAEAAKKFQHSLELIEKSSLSADVKEDAKLAAHFNNG
                     RVALAKGDIATAKKEADAYASGAEARHNQFRIKGAHELKGAIALKEKNFDTAINELGE
                     GNQQDPQVLYFTALAYQGKGDTAKAKEYASKAANFNVLPFLPYAFVRAKASKLEAAS"
BASE COUNT          345 a          727 c          754 g          420 t
ORIGIN      
        1 ccctgatctc gctcgccatc tcggtcgcct ttctcttcag tgtcgccgta acgctgggct
       61 atccaggggc gcccctctgg gaagagctct cgagcctagt caccatcatg ctcctgggac
      121 actggatcga gatgcgctcg atctcgcagg cccagggcgc gctcggcgag cttgcaaagc
      181 tccttccggc cgcggtggta cgggttcgcg gcaccgacac cgaggagatt ccgatcagcg
      241 agctgcgcga gggcgacgtc gtcctggttc ggcccggcgc cagtgttccg gccgatggtc
      301 tgatccaaca gggcgagagc gcgctcaacg aatcgatgat caccggcgag tcgagcccgg
      361 tggacaagaa gccgggtgac cgcgtgatcg cgggcacgat caacggctcg ggctctctcc
      421 acgtcgaggt gacggggacc ggtgagcgga ccgcactcgc cggaatcatg cgcttggtgg
      481 cccaggccca aacctcccgt tcccgggcgc aggcgctcgc cgaccgcgcc gcattctggc
      541 tcaccgtgat cgcgctcggc gtcggcgtga tgaccttggt ggcgtggctc gctgtcggtg
      601 ccggcgctgc ttttgccgtc gagcgactcg tgaccgttct cgtgatcgcg tgcccccacg
      661 cgctcggcct cgccattcca ctggtcattg cgatctcgac caccctgggc gccggtgccg
      721 gtctcttggt gcgagaccgg cgggggctgg aggaggcacg gaacctcacg accgtggtct
      781 tcgacaagac gggaacgctc actcgaggtg agtttggcgt ggtcgccatc cgcaccgtcc
      841 agggtcttgc agagaccgaa gcactccggc tcgcggccgc ggtcgagcga gactccgagc
      901 acaccatcgc tcagggaatg ctgcggagcg cacgagagcg aaacctgagc gttccgccgg
      961 cccaggactt ccaggcgctc cccggcctgg gcgtccaggc tcgcgtggat ggccggaccc
     1021 ttctcgtcgg ggggccgaac ctcctgcgaa agcggtcgat tgcagtgccc gtggagctgc
     1081 aacgggccgt cgacgaagct gcggcacagg gacggacggc ggtctatctc gtcgaggacg
     1141 ccggagggag tcgagccacc gtgctcgcca ccttggtagt cgctgacgtc gtgcgggaag
     1201 aatcgaagac tgccgtccgc gagctgcacg accagcgcat caaggtcgtt atgctcaccg
     1261 gagatgcccg gccggtcgcg caggcggttg cccaggagct cggcattgac aacgtgttcg
     1321 ccgaagtctt gcccgagcag aaagtcggcg tcatcgagca gctccgaagc aggggcgagc
     1381 gggtcgcgat ggtgggcgat ggagtgaacg acgctccagc gctgctgact gcggatgtcg
     1441 gcgttgcgat cggagcaggg accgatgttg cggtcgaagc gggagacatc gtcctggtcc
     1501 ggagcgaccc gcgggacgta tctcggattg ttcgactcag ccgcgccagc taccggaaga
     1561 tggtgcagaa tctctggtgg gctactggct acaacgtggt cgcgatcccg ctggccgccg
     1621 gagttctggc gcggtacggg atcctgctct cgcccgcgat tggggcggtg ctcatgtcat
     1681 tgagcaccgt catcgtggca gtcaatgcac aactgctgcg tcgggcgcgg atatgatccg
     1741 cgcccgttac agcacgccaa caatgtttac gacgcagcct ccagcttgct cgccttggct
     1801 cgcacgaacg catagggcag gaacggcagc acgttgaagt tcgcggcctt gctcgcgtac
     1861 tccttggcct tcgccgtgtc acccttcccc tgatacgcca gcgccgtgaa gtaaaggacc
     1921 tgcgggtcct gctgattgcc ctcgccgagc tcgttgatcg cggtgtcgaa gtttttctcc
     1981 ttgagcgcga tggcaccctt gagttcgtgg gcccccttga tccggaactg gttgtgccgc
     2041 gcctcggcgc cactcgcgta tgcgtctgcc tctttcttgg cggtcgcgat gtcacccttg
     2101 gccagcgcca cccggccgtt gttgaaatgg gccgcgagct tcgcatcttc cttcacgtct
     2161 gctgagagac tcgatttctc gatcagctcg agcgaatgct ggaacttctt ggcggcctcg
     2221 gccgtgcggc ccgcgtcgag caggat
//
LOCUS       DASRJQ010000486         4899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3623680, whole genome shotgun sequence.
ACCESSION   DASRJQ010000486 DASRJQ010000000
VERSION     DASRJQ010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3623680"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            409..810
                     /locus_tag="VFB89_11115"
     CDS             409..810
                     /locus_tag="VFB89_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZM27899.1"
                     /translation="MSKPTTAARFHRWEDLPREKVTDLLGRRLICGDRMMLAHVYLKK
                     GCIVPAHSHDNEQLTYVLEGALRFWLGEDGSEEVVVHGGEVLVIPSNLRHKAEALEDT
                     LDVDVFCPPRQDWLDKTDTYLRAGVDDLFRG"
     gene            complement(814..4014)
                     /locus_tag="VFB89_11120"
     CDS             complement(814..4014)
                     /locus_tag="VFB89_11120"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HZM27900.1"
                     /translation="MQRARRLPLLGALILLAGLVPFRSIPAQDTTKGPPPFEDLKFRS
                     IGPAAGGRVSRVSGVPGDPLTYYAATSAGGVWKSSDGGRSWRSVFDGQPISSIGSIAV
                     APSNPNVIYVGSGEANIRGNVGAGNGIYKSTDAGKTWTHVWIQEGQIGTMVVDPTNPD
                     VAFAAVLGHAFGPNRERGVFRTRDGGKTWQLVLRKDQDTGASDVALDPRNPSIVFAGL
                     WQARRRPWELVSGGAGSGLYVSRDGGDTWKQLTDKGLPSGIWGKVGVAVAPSDGRRVY
                     ALIEADSGGLFRSDDGGETWKRASGHHALRQRAWYYTTLTIDPRNPDVVWFPEVNLLK
                     TIDGGKTITSVRGPHHGDHHDVWIDPKDPTRIIEGNDGGVDLSLDHGEHWFAPPLPIA
                     QFYHVSADNAVPYHVMGAMQDLGTAWGPSNSLVADGLRLAHWRSVGGGEAGYVVAEPS
                     DTNVIYAGEYSGYISHYDLRSRQPRNVSAYPENGSGHGGEDLQYRFQWTAPIATSPNQ
                     PNVVYHGANVLFKSTDAGQTWTPISPDLTRNDKTKQRWSGGPITGDNTGVEIYGTIFT
                     MAESPKEAGLIWVGSDDGLVQLTRDGGKSWTNVTKNVPGLPEWGTVSLIEPSPFDAAT
                     AYLVVDAHRLDDMRPYLWKTSDYGKSWKRLTSGLPQDIYLHAVREDPARKGLLFLGTE
                     RGVMMSPDDGASWQPLRLGLPTVAVHDLVVKGNDLVLATMGRSLWIFDDITALREISP
                     KVREEPVHLFTPQTATRWRVRSEWHTEEDAKEPGTADNPPRGALVYYYLKTKPKAEIR
                     LEILDQQGAVIRRLRSTPEPPEYAPDDPDEPKDAPKPELVADSGVHRAVWDLRYEGAE
                     KIKPGKLDGGTPDEGPIALPGDYTVRLVVEGKSLTAPLKVLPDPRVKLPPQALADQLK
                     FSLELRDQITRVTGLVDDIRSLRRQLAVRSDLWKDQPKAANLVSGAQTLTVKLDTLEA
                     RVHNPRAEVVYDILAQRGGTKIYSRLAPLYTSVIEADGAPTQGMREVYATLKKELDAA
                     DAEFRTLVSGDLAALNRSARELNFGDVLVPAARPGR"
     gene            complement(4090..4422)
                     /locus_tag="VFB89_11125"
     CDS             complement(4090..4422)
                     /locus_tag="VFB89_11125"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YXWGXW repeat-containing protein"
                     /protein_id="HZM27901.1"
                     /translation="MSPRVFPHPLRRSLSPILFLSGLLASACVVGPPAGRVYVVPAPP
                     PPRAEIVTVAPGPGYVWVGGRWRWNGSAYYWVPGSWVAPAYGRYRWVPGHWAEDRRGW
                     YWVEGHWR"
     gene            4643..>4899
                     /locus_tag="VFB89_11130"
     CDS             4643..>4899
                     /locus_tag="VFB89_11130"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZM27902.1"
                     /translation="MLAFMRAAPTTYILHWQDGRLRRQGAGLSFVYFAPTSTIAAVPL
                     ASTDVPFVFTESTADFQTVTLQGSLTYRVSDPARLAAALDY"
BASE COUNT          900 a         1579 c         1539 g          881 t
ORIGIN      
        1 tgggccagat ccgcggctcg catcgccctc gacgtttcaa tcaggcctgc ccgaaacgcc
       61 gcttctccga gcaccgcact cgcatttccg ccgccggcgg ggagatcacg aatgccggta
      121 tcaccgctgc ccttgctcag cttgtctccc gatggtttgc gaagcagggg tgatgtacga
      181 acaccggcgg atccaggcgt ccgagcatgc gggcaagtcg tatctgccgg ccggtcgatg
      241 gaagcaagtc ctcttgacga cgtgtccgag atgcaggaac ccggtcgggc tcggcgcgaa
      301 gcgggtgcgt ggggaaaccg gaagccgctg acggaatcgg tcgagatcga gcggtagcac
      361 ggcgggaagg taatattgac gaggtcaccc taaccgaaga gtgtcgtcat gtccaagccg
      421 accaccgctg cacgcttcca ccgctgggaa gatctccctc gcgaaaaagt caccgacctg
      481 ctcggtcgcc ggctaatctg cggcgaccgc atgatgctcg cccacgtgta cctgaaaaag
      541 ggctgcatcg tgccggccca ctcacatgac aacgaacagc tgacctacgt gctggaggga
      601 gcgctccggt tctggctggg cgaggacggc agcgaggagg tcgtggtgca cggcggcgag
      661 gtcctggtta tcccgagcaa tctgcggcac aaggcagagg cgctcgaaga cacgctggac
      721 gtcgacgtct tctgcccccc gcgccaggac tggttggaca agaccgacac ctatctcagg
      781 gccggcgtcg acgatctgtt ccgcggctga gggctagcgg ccgggacgcg ctgcgggaac
      841 cagcacgtcg ccgaagttga gctctcgcgc gcttcgattg agcgcagcca gatctccgga
      901 tacgagggtg cggaattctg cgtcggcggc gtcaagctcc ttcttcaacg tcgcgtacac
      961 ttctcgcatg ccttgagttg gagccccatc ggcctcgatg accgaggtgt acagcggcgc
     1021 caaccgggag tagatcttgg tgccgccgcg ctgggccagg atgtcataca ccacttccgc
     1081 ccgcggattg tgcacccggg cttccagcgt atcgagctta acggtgagcg tctgcgcgcc
     1141 acttaccagg ttcgcggcct tcggctggtc tttccaaaga tccgaccgta cggcgagctg
     1201 gcggcgcaac gagcgaatat cgtcgaccag cccggttacg cgcgtgatct gatcgcgaag
     1261 ctcgaggctg aacttcagct gatccgcgag cgcttgcggc ggcagtttca cccggggatc
     1321 cggcagcacc ttcaagggag ccgtgaggct cttaccctct acgaccagtc gtacggtgta
     1381 gtcacccggc aaggcaatcg ggccttcgtc aggggttccc ccgtccagct tgcccggctt
     1441 gatcttttca gccccctcgt agcgaaggtc ccaaaccgcg cggtggactc ccgaatccgc
     1501 aaccagctcg ggcttgggcg catccttcgg ctcatccgga tcgtcgggag catactccgg
     1561 cggctccggt gtgcttcgga gcctgcgtat tacagcgccc tgctgatcca ggatctcgag
     1621 tctgatctcg gccttgggtt tggtcttcag atagtagtag accagcgcac cgcgaggcgg
     1681 attgtcagcc gtgcctggtt cctttgcatc ctcttccgtg tgccactcgc tccggacccg
     1741 ccaacgcgtc gcggtctgcg gcgtgaacag gtggacaggc tcctcgcgca ccttcgggga
     1801 aatctcccga agcgctgtga tgtcatcgaa gatccaaagg gaacggccca ttgtggccag
     1861 cacgagatcg ttccccttca ccaccaggtc gtgaactgcg accgttggaa gtccgagtcg
     1921 caacggctgc caggacgctc catcgtcagg cgacatcatc actccgcgct cggtgcccag
     1981 aaagagcaga cccttgcgcg ccgggtcctc ccggaccgcg tggaggtaga tgtcctgcgg
     2041 aaggccgctg gtcagtcgct tccaactctt gccgtagtcg ctggtcttcc acaggtacgg
     2101 ccgcatgtcg tccaggcgat gcgcgtccac cacgaggtac gcggtagcgg catcgaacgg
     2161 cgacggctcg atgagactta cggtccccca ctcgggaaga ccgggaacat tcttcgttac
     2221 gttggtccag gacttcccgc cgtcgcgggt gagctggacc aggccgtcgt cactgcccac
     2281 ccaaatgagg cccgcttcct tcggcgattc cgccatggtg aaaatggtcc cgtagatctc
     2341 gacgcccgtg ttatcgccag tgatcgggcc gcccgaccaa cgctgcttgg tcttgtcgtt
     2401 gcgagtcaga tccgggctga tcggcgtcca ggtctggccg gcatccgtgg acttgaagag
     2461 tacgttagcg ccgtggtata cgacgtttgg ctgattgggc gaggtcgcaa tgggagcggt
     2521 ccactgaaag cgatattgca ggtcctcgcc accgtgaccg gatccgttct caggatacgc
     2581 cgagacattg cgcggctgcc gcgaacgcag atcgtagtgc gagatgtatc cgctgtactc
     2641 gccggcgtag atcacattgg tgtcgctcgg ctcggcgacg acgtagcccg cctcgccgcc
     2701 accgaccgaa cgccaatggg caagccgcag tccgtcggca accaggctgt tgctcggccc
     2761 ccaagcggtt cccagatctt gcatggcgcc catgacgtgg tagggcacgg cattgtcggc
     2821 cgatacgtga tagaactggg cgatgggcag cggcggcgcg aaccagtgct cgccgtgatc
     2881 caaggagagg tcgactccac catcgttccc ctcgataatg cgggtcggat ccttcggatc
     2941 gatccacacg tcatggtggt cgccatgatg ggggccgcga accgaggtga tcgtcttacc
     3001 gccgtcgatg gtctttagga ggttgacctc ggggaaccac accacatcgg gatttcgtgg
     3061 atcgatggtc agtgtggtgt agtaccaggc acgctggcga agcgcgtgat gcccgctggc
     3121 tctcttccag gtctcgcccc catcatcgga gcggaatagc ccaccgctgt cggcttcgat
     3181 cagcgcatag actcggcgcc cgtccgatgg ggcaacggct acgcccacct tcccccaaat
     3241 gccgctcggc agacctttgt cggtcagctg cttccaggtg tctcctccgt cgcgagacac
     3301 ataaagcccg ctgcccgctc cgccgctcac cagctcccag gggcggcgac gcgcctgcca
     3361 aagcccggcg aagacaatgc tggggtttcg tggatcgagg gcaacgtctg acgcaccggt
     3421 atcctgatcc ttgcgcagta ccagctgcca ggtcttccca ccgtcgcgcg tgcggaacac
     3481 acctcgctcg cgattagggc caaaggcgtg gcccagcacg gcggcgaaag caacgtcggg
     3541 attggtcgga tccaccacca tcgtgccgat ctggccttcc tggatccaca cgtgcgtcca
     3601 tgtcttaccg gcatcggtgg acttgtagat gccgttgccg gctcccacgt tgccgcgaat
     3661 gttcgcctcg cccgagccca cgtagatgac gttggggttg gacggcgcta ccgcgatcga
     3721 gccaatagaa gagatcggct gaccgtcgaa aacggagcgc catgaacgcc caccatccga
     3781 agacttccac acgccgccgg ctgaggtggc ggcgtaatag gtgagcggat cgcccggcac
     3841 accgctcacg cgggaaacgc ggcctcccgc ggcgggcccg atcgaacgga acttgagatc
     3901 ctcgaacggt ggcggacctt tggtggtgtc ttgcgcggga atgcttcgga aaggaacgag
     3961 ccctgcgagg aggatcaggg cgcctagtag cggaagtcgt cgggcgcgtt gcatgacaaa
     4021 agtctcgctg aggtggcgga caatctaatc gcctcagcga gacgcgaagc catcactcgt
     4081 accgatcgtt cagcgccagt gtccctccac ccagtaccaa ccgcgacgat cctccgccca
     4141 atggccgggc acccagcggt agcgtccgta ggcaggagca acccacgagc ccgggaccca
     4201 atagtacgcg cttccattcc aacgccaccg cccgccgacc cagacgtagc ccggacccgg
     4261 ggcaactgtg acgatctcgg cgcgcggcgg tggcggagcc ggaacgacgt agacacggcc
     4321 cgctggggga cccaccacgc aggctgaagc aagcagacct gagaggaaca ggatcggact
     4381 caaactgcgg cgaagcggat gagggaacac acgaggcgac atgaaaacct ctcgaagacg
     4441 ttcggccagt ccagcgggaa gcggagccgg ctcttggccg acgcgaggcg ggcacgcggt
     4501 gttaaggact cgtcaacatc cgcgctatca ccgtataagc cagtgtggtt atagggttta
     4561 cgaacagggt cggcggccgc gcttgacata gtgtctgcaa tacactaata tcgtgtcata
     4621 gagacactaa ctggagggta ccatgctggc attcatgcgg gcggctccca ccacctacat
     4681 cctccactgg caggatggcc ggctgcgccg gcaaggcgcg ggactctcct tcgtctactt
     4741 cgcgccgacc tcgactatcg cggcggtgcc gctggccagc acggatgtcc cgttcgtctt
     4801 caccgagtcc acggccgatt tccagaccgt gacgctgcaa ggaagcctca cctaccgggt
     4861 atctgaccct gcccggcttg cggcggcatt ggactacag
//
LOCUS       DASRJQ010000487         4727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1162166, whole genome shotgun sequence.
ACCESSION   DASRJQ010000487 DASRJQ010000000
VERSION     DASRJQ010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162166"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VFB89_11135"
     CDS             complement(<1..408)
                     /locus_tag="VFB89_11135"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZM27903.1"
                     /translation="MSKRSDALAERLEQGAEALAALARSLTDAEWQVRIPKDGRKVGV
                     VVHHVATMYPLEIQLAQALAAGKPIEGVSWDDVHAINSKHAKEHDAVTKEAALDLLRR
                     SSTAAAAAIRALSDQELDRAATVSLNANAPLTCQ"
     gene            complement(485..946)
                     /locus_tag="VFB89_11140"
     CDS             complement(485..946)
                     /locus_tag="VFB89_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZM27904.1"
                     /translation="MRTLPLSACLLLLATSLYGQGTSNHQGSAPVWGPAPAVFPSGAK
                     MAVISGDPGKAAPFTIELSMPDGYRIPPHFHPTDEAVEVKQGTFLVGMGDSLDVAKTK
                     AMKVGDKGQVPAKAHHYAAAKGATIVSVTAMGPFAMTYVNPADDPQKAAKP"
     gene            complement(976..1809)
                     /locus_tag="VFB89_11145"
     CDS             complement(976..1809)
                     /locus_tag="VFB89_11145"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZM27905.1"
                     /translation="MTGMQQQVDSQPDEALRARVRATWTAGDFGRIAVGFESGAADFI
                     AQLALKQGEDVLDVACGTGNLTLPAARAGARVTGIDIAPGLIAQLQARAVAEGLAVDA
                     REGDAEALPFADASFDTVVTMFGAMFAAHPQQAADELLRVTRPGGRIAMANWAPEGFI
                     GSMLKVTVRYLPPPPGVPSVLLWGNPGVVEERLGAGLSDLRFERRSMVLAYPFEPEQT
                     VELFRTWYGPTVRTFAALDEAGQRGLFADLVRLWTEHNTAGPNGTRVESEFLAVVGIR
                     A"
     gene            1945..2694
                     /locus_tag="VFB89_11150"
     CDS             1945..2694
                     /locus_tag="VFB89_11150"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HZM27906.1"
                     /translation="MTECPPRSYGSFCPVAQASEVVAQRWVPLILREIMVGYHRFNEI
                     RHALPLISPSVLSQRLRSLEDDGVIYRRIESGGVTYHLTPAGEELRPIVDSLGHWARK
                     WVNRDYRSYELDPGVMMWSLRRHVMTEEFPSGRTVVHFELEGAPRLRRYWWLVVQREA
                     RDVDVCMTDPGHPVTLTVTAKLRSLIDVLMGDARLKDVLREGLLSIEGERELVRRFER
                     LFEFEGGAHSFVGGGHQKAVEPAKPRRVGAL"
     gene            complement(2771..2983)
                     /locus_tag="VFB89_11155"
     CDS             complement(2771..2983)
                     /locus_tag="VFB89_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27907.1"
                     /translation="MADVNDCIPGRRARVLQAGVARVAGKTGTIVEVTRTRRPSTGPL
                     VDRITVDVPGHGEIVLSPKDLEILPD"
     gene            complement(3015..3776)
                     /locus_tag="VFB89_11160"
     CDS             complement(3015..3776)
                     /locus_tag="VFB89_11160"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZM27908.1"
                     /translation="MPSATQLALVTGTTSGIGLAVARQLLDSGWTVGGIARRAAEISH
                     PRYHHIAADLAELSSSVASLEAAIQRLLGDHQPWQRVGLVNNAASADLLTSFEAIDPL
                     ALLRLYAVNTAAPVWLMGLVVRESPHEAVVRIVNVSSGAAVGVFPGLAAYGSSKAALR
                     MAGMVAAAELASPQRRQSAPTDVAILSFEPGIVDTPMQQVARGQPPEKFPSVHLFRDI
                     AARGVLVPPEAPAAEIVAFLESDRQPGFAERRFGA"
     gene            complement(3769..4416)
                     /locus_tag="VFB89_11165"
     CDS             complement(3769..4416)
                     /locus_tag="VFB89_11165"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HZM27909.1"
                     /translation="MLQAYKLTIPQSLKEACDPARLALIVYDMQVGILSQITDGAAVT
                     ANVGAVLRAARSAGVRVLFSRHLSLPKELMGVSQLRMAMAWQRVGSVEEVRPWFLRDS
                     PGFQLLPEMTPRESEAVFDKITMSAFEGTPLDITLRDCGVNAFLIAGVATEVGIEPTV
                     RHGADLGYIPIVVTDACGAGHADAAERALATLRYAGDALFTTVAEICDVLSGVRA"
     gene            complement(4487..>4727)
                     /locus_tag="VFB89_11170"
     CDS             complement(4487..>4727)
                     /locus_tag="VFB89_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27910.1"
                     /translation="LALPPGWRAELPRTVSVDGKWGRYATRYTQDGQTLHVLREFEGA
                     RGVYLPAEVGDLVSWLRAVAADDVPYIVIESGASP"
BASE COUNT          790 a         1578 c         1556 g          803 t
ORIGIN      
        1 ctggcaggtg agcggggcgt tggcgttgag cgagaccgta gcggcgcgat ccagctcctg
       61 atcgctgaga gcccggatgg cggccgcggc cgcggtgcta ctgcgccgaa gcaggtcgag
      121 cgccgcctcc ttggtgaccg cgtcgtgctc cttggcgtgc ttcgagttga tcgcgtggac
      181 gtcatcccag ctcacgccct cgatcggctt gcccgcggcc aacgcctgtg ccaactggat
      241 ctccagcgga tacatggtcg cgacgtggtg cactacgaca cccaccttgc gtccatcctt
      301 ggggatgcgg acttgccact ccgcatcggt cagagagcgg gccagggccg cgagggcttc
      361 ggccccctgt tccaggcgct cggcaagggc atcggaacgc ttactcatgt gctcacctct
      421 aggtaatggg tatcgagttg ctctcaggcg actcgttctc gctcgcattg aaccaacctt
      481 catcctacgg ttttgccgcc ttctgaggat cgtccgccgg gttcacgtac gtcatggcga
      541 agggccccat cgccgtcacc gatacgatcg ttgctccctt ggcggccgcg taatgatggg
      601 ccttggcggg tacctgcccc ttgtcgccca ccttcatggc cttggttttc gcgacatcca
      661 gcgagtcacc catccccacc aggaacgtgc cctgcttcac ttccaccgcc tcgtcggtgg
      721 gatggaagtg gggcggaatc cggtatccgt ccggcatcga cagctcgatt gtgaacggag
      781 cagccttgcc cggatctccg ctgattaccg ccatcttcgc cccgctcggg aacacggcgg
      841 gcgcgggccc ccagaccggg gcggaccctt gatggtttga cgtgccctgc ccatacaacg
      901 acgtggccaa cagcagcagg caggctgaga gcggaagtgt gcgcatgtcg ttctccgatc
      961 aggtctcacg aggtgtcagg cgcgaatgcc gactaccgca aggaactcgg actcgactcg
     1021 ggttccgttc gggccggcgg tgttgtgctc agtccagagt cgcaccaggt cggcgaaaag
     1081 gccgcgctgc ccggcttcat ccagggccgc gaaggtgcga acggtcggcc cgtaccacgt
     1141 gcggaaaagc tccaccgtct gctcgggttc gaaggggtac gccagcacca tgctgcgtcg
     1201 ctcgaagcgc aagtcgctca gtccggcgcc cagacgctcc tccacaacac caggattgcc
     1261 ccagagcagc acggacggca caccgggtgg aggcggcaag tagcggacgg tcaccttcag
     1321 catgctgccg atgaagcctt ccggagccca gtttgccatg gcaattcgtc cgcccggacg
     1381 ggtgacccgc agcaactcgt ccgcggcctg ctgcggatgg gcagcgaaca tggcgccgaa
     1441 catcgtcacc acggtgtcga agctcgcgtc ggcaaacggg agcgcctccg cgtctccctc
     1501 ccgcgcatcg accgcgagtc cctccgctac ggcgcgggcc tggagttgag cgatcaagcc
     1561 cggtgcaatg tcgattccgg taacccgcgc tcccgcccgc gcggcaggga gtgtcaggtt
     1621 tccggtccca caggcgacgt cgagcacgtc ctcgccctgc ttcagcgcga gctgggcaat
     1681 gaaatcagcc gccccacttt caaagccgac cgcgatgcgg ccgaagtcac ctgcagtcca
     1741 ggtggcgcgt actcgcgcgc gcagcgcctc atcgggctga gagtctactt gctgctgcat
     1801 cccggtcatc gacgttcctc cctgagtcgc tagccgggcc gcaccacggg agccgccggc
     1861 cggctccggc gacggcgaag ggtacgaatt gccatacggg ccaacaagta tgagattcgt
     1921 accgccctcg acccgagtga cctgatgaca gaatgtcctc cgcggagcta cggctcgttc
     1981 tgccccgtcg cgcaggcctc cgaagtggta gcccagcgct gggttccgct gattcttcgc
     2041 gagatcatgg tggggtacca tcgcttcaac gagattcgcc acgccctgcc gctgatctcc
     2101 ccgtcggtgc tctcgcagcg gctccggtcg ctggaagacg acggcgtgat ctatcggcgg
     2161 atcgagtcgg gcggcgtcac ctaccacctc actccggccg gcgaggaact ccgaccgatc
     2221 gtggactcgc tcggtcactg ggcccggaaa tgggtgaatc gcgactaccg gtcgtacgag
     2281 ctggaccccg gcgtgatgat gtggtccctc cgccggcatg taatgaccga ggagttccca
     2341 tcgggccgaa ccgtggtgca cttcgagttg gaaggcgcgc cgaggcttcg ccgttactgg
     2401 tggctcgtgg ttcagcgcga ggcgcgggac gtggacgtgt gcatgaccga tcctgggcat
     2461 cccgtcaccc tcacggtcac ggcaaaactc cgctcgctga tcgatgtgct gatgggcgac
     2521 gccaggctca aggacgtcct gcgggagggc ttgctgtcga tcgagggaga gcgtgagctg
     2581 gtgcggcggt tcgagcggct gttcgagttc gaagggggtg cccactcgtt cgtcggagga
     2641 gggcaccaga aagcggtcga gcccgccaag cccaggcggg tcggcgcact ctagcgccgc
     2701 gccctaggac tgcgccgcgc ggcggggctt cccgccaggc ccgggtgctt ggctgcctcg
     2761 cccagctggg tcagtccggg agaatctcga ggtctttagg gctcaagacg atctcgccgt
     2821 ggccgggcac gtcgactgta atacgatcga ccaggggacc ggtggaaggg cgtcgagtgc
     2881 gggtgacctc gacgatggtg cccgtcttcc cggccacgcg ggcaacgccg gcctggagca
     2941 cgcgggcgcg gcgaccgggg atgcagtcat tgacatcggc cattgatgtt ctcctctaac
     3001 gccctcccgg tcgctcacgc gccaaagcgg cgctcggcaa aaccaggctg tcgatccgac
     3061 tcgagaaagg caacgatctc ggcggccggt gcttcgggcg ggacgaggac cccgcgggcc
     3121 gcaatgtcac gaaacagatg caccgaggga aatttctcag gcggctgccc gcgcgccacc
     3181 tgttgcatcg gcgtgtctac gattccgggc tcgaagctca ggatggccac atcggtcggg
     3241 gccgattggc gccgctgcgg agatgccagc tctgcggcgg ctaccatgcc ggccatgcga
     3301 agcgcagcct tgctgctccc gtacgcagcg agcccgggga agacacccac cgcggcgccg
     3361 gaggacacgt tgacgatccg gaccactgcc tcatgcgggc tttcccgaac gaccagtccc
     3421 atcagccaga ccggggccgc ggtgttgacc gcatagagcc ggagcagtgc gagagggtcg
     3481 atcgcctcga acgaggtgag gaggtcggcg ctggccgcgt tgttgaccag accgacgcgt
     3541 tgccaaggtt gatgatcgcc caacagccgc tgtatcgccg cctcgagcga agccacgctg
     3601 gacgacagct cggccaggtc ggcagcgatg tgatgatagc ggggatggga tatctcggcg
     3661 gcgcgccggg cgataccgcc gacagtccag ccgctgtcga ggagctgtcg ggcgacggca
     3721 aggccgatac cggacgtggt gccggtgact agggccagct gagtcgcgct aggcacggac
     3781 cccgctcagc acatcgcaaa tctccgctac ggtcgtgaac agcgcgtccc ccgcataccg
     3841 caaggtcgcc agcgcccgct cggctgcatc ggcgtgaccc gccccgcagg cgtcggtgac
     3901 gacgatgggg atatatccga ggtctgcgcc gtgacggacg gtgggctcga tcccgacctc
     3961 ggtcgcgact cccgcaataa ggaaggcgtt gactccgcaa tcacgcagcg tgatgtccaa
     4021 cggcgtgccc tcgaacgccg acatggtgat cttgtcgaaa accgcctcgg attcacgtgg
     4081 cgtcatttcg ggcagcagct gaaatcccgg cgagtcgcgc agaaaccaag gccgcacttc
     4141 ctcgacgctg ccgactcgct gccatgccat tgccatgcgc agctgcgaga cgcccatcag
     4201 ctccttcggc aacgacagat ggcgggagaa gaggacgcgc actcctgcgc tacgcgccgc
     4261 ccgcaacacc gcgccaacgt tggccgtcac ggcggcgccg tcggtgatct ggctcaggat
     4321 gccgacctgc atgtcgtaga cgatgagagc caggcgcgcc gggtcgcacg cctccttcag
     4381 gctctgggga atggtgagct tatacgcctg tagcactggt cacctcatgc cgcccgctgc
     4441 tgcgggcccg ttcgtgacca ggaattctac ttgggttgtc gcggcatcac ggtgaggcgc
     4501 ccgattcgat cacgatgtag ggaacgtcat cggccgcgac ggctcggagc catgagacca
     4561 gatcccccac ctctgcgggt agatagaccc cgcgggctcc ctcgaattcc cgcaatacat
     4621 gaagcgtctg tccgtcctgg gtgtagcggg tcgcgtaacg tccccatttc ccatcgacgc
     4681 tgacggtgcg cggcagctcg gcccgccaac ccggtggtag cgccaag
//
LOCUS       DASRJQ010000488         2914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3672715, whole genome shotgun sequence.
ACCESSION   DASRJQ010000488 DASRJQ010000000
VERSION     DASRJQ010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3672715"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VFB89_11175"
     CDS             <1..439
                     /locus_tag="VFB89_11175"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZM27911.1"
                     /translation="LIEHLPAMLWDVAVPGIPRRTIRAIAAHLHNARCSWIKTLGREH
                     GIPTPSRVDHRHVTRRQLVAALKRSSSGMEALLELGFAAGGQVPPSKGYVWRNLSLDV
                     AHVLTYFVAHEAHHRGQITMVARQMGQRLPTATSAGLWQWKTP"
     gene            424..627
                     /locus_tag="VFB89_11180"
     CDS             424..627
                     /locus_tag="VFB89_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27912.1"
                     /translation="MEDAVECATSPEWWAQLTSDGILFRASAAGLAIAEAVGIVVAIA
                     GLRRWAGARDGRPNREVNIQEEP"
     gene            627..1031
                     /locus_tag="VFB89_11185"
     CDS             627..1031
                     /locus_tag="VFB89_11185"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HZM27913.1"
                     /translation="MDSAAERKRLLERDAQWAAAAAEGRDVERILEFWTDDALVYPPG
                     FPPVAGKAALRAYVHASLAIPGFRITWTSSDVRLSPDGQLAYMLSENIVTAPDPTGQL
                     ATSRGRAVTIWRRESDGEWRCSVDIWNPGAPG"
     gene            1053..1571
                     /locus_tag="VFB89_11190"
     CDS             1053..1571
                     /locus_tag="VFB89_11190"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZM27914.1"
                     /translation="MLPTTARVALRELAPGDLDFLVEMLSDPEVMRHYPRCLTRPEAE
                     AWIERQLERYRQDGHGLWLASLHRTGEPIGQVGLLRQVLPDGAEWEIGYLLHQKFWHQ
                     GYATEAALAVREYAFRVLMPRRVISLIRPANMPSQRVARRLGMNPVARVQFSGLDHDV
                     FAAMRGQGEVCP"
     gene            1601..1927
                     /locus_tag="VFB89_11195"
     CDS             1601..1927
                     /locus_tag="VFB89_11195"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HZM27915.1"
                     /translation="MPNDRIRAQIAPSGEHHEGREAVHDYYEDLLTSLPDLHIDITHC
                     LGADEGVALEVRISGTHLAPWRGLPPTGRRVSFPLCGMFTLDQEGKLAGGRIYYDRGR
                     VLRRAS"
     gene            2012..2338
                     /locus_tag="VFB89_11200"
     CDS             2012..2338
                     /locus_tag="VFB89_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27916.1"
                     /translation="MTFKPAIWYPIAAGLSVLNVVAVGFAAAAAEPWHAGIHATLGLA
                     FGLWAQRLRRSYSGSENVLEPRIEELEIEMGRLRQELTETQERLDFAERVLAQPQETR
                     RVGPQP"
     gene            2356..2877
                     /locus_tag="VFB89_11205"
     CDS             2356..2877
                     /locus_tag="VFB89_11205"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZM27917.1"
                     /translation="MQVAASADLSAQTLDDILALCERAYGEDLRPSFLTFTDPTHVMA
                     FADGAVLSHAMWVTRWLAPGASPPLRAAYVEAVATDPAYQGRGLATEVMRVLVSRLSS
                     FQLAALCPSDRGQSLYERLGWEPWRGPLFIRQERHLIETPDEAVMIHRLPASPPLNLD
                     DPLSAEWRPGELW"
BASE COUNT          508 a          929 c          946 g          531 t
ORIGIN      
        1 ccttatcgag cacttgccgg cgatgctctg ggacgtcgct gttccgggca tcccgcggcg
       61 cacgatccgc gctatagcag cgcacctcca caacgcccgc tgcagctgga tcaagacgct
      121 cgggcgggag catggcattc cgactccctc ccgcgtcgac caccgtcatg tcacccgtcg
      181 ccaactcgtc gccgcgttga aacgcagtag cagcggaatg gaggcgcttc tcgagctcgg
      241 ctttgccgcg ggaggtcagg tcccgccatc caagggctac gtgtggcgca atctctccct
      301 cgacgtagcg cacgtgctga cctacttcgt ggcgcatgaa gctcatcacc ggggtcagat
      361 cacgatggtg gcgcggcaaa tgggccagcg cttgccaacc gcgacctccg ccgggctgtg
      421 gcaatggaag acgccgtaga gtgcgcgacg tcgcctgagt ggtgggccca gctcacgagc
      481 gatggcattt tgttccgggc ttctgcggca ggcctggcga tcgctgaagc ggtgggcatt
      541 gtggtggcaa ttgctggatt acggcgctgg gcgggtgccc gggatgggag acccaaccgc
      601 gaagtcaaca tacaagagga gccctgatgg actccgcagc ggagcggaaa cgtctgctgg
      661 agcgcgacgc ccagtgggcg gccgcggcgg ccgaaggccg cgacgtcgag cgcattctcg
      721 agttctggac agacgacgcg ctagtctacc cgccaggctt cccgcccgtt gccggcaagg
      781 ccgcgttacg cgcgtacgtc cacgcgagcc tggcgattcc cggtttccgg atcacctgga
      841 cctcgtctga tgttcgcctg tcgcctgacg gccaactcgc ttacatgctg agcgagaaca
      901 tcgtcaccgc accggatccc accggtcagc tggcgacaag ccggggccgg gccgtcacca
      961 tctggcgccg agagtcggac ggcgagtggc gctgttccgt cgatatctgg aaccctggtg
     1021 caccaggtta agtacgcggc gtgcgagttg cgatgttgcc caccaccgcc cgagttgccc
     1081 tccgcgagct tgcgccaggc gacctcgact ttctcgtcga gatgctgtcc gatccggagg
     1141 taatgcgtca ctaccccagg tgccttactc gcccagaggc ggaggcgtgg atcgagcgac
     1201 agctggagcg gtaccgccaa gatggacacg gcttgtggct cgcttccctg caccggaccg
     1261 gcgaaccgat cggccaggtc ggattattgc gacaggtcct gccggacggc gcagaatggg
     1321 agatcggcta tctgctgcat caaaagttct ggcatcaggg gtacgcgacg gaggctgccc
     1381 tcgccgtacg agagtacgcc tttcgcgtgt tgatgccgcg cagggtaatc tctctgattc
     1441 gtccggcgaa catgccgtcg cagcgcgtgg cccgacgcct cggcatgaac ccggtcgcgc
     1501 gggtccaatt ctccggcctg gaccacgacg tgttcgccgc gatgcgtggt cagggtgagg
     1561 tatgtcctta aggccctgtt atcccgaaga tatgatggcc atgccaaacg atcgaatccg
     1621 cgcccagatt gcgccctcgg gtgaacacca cgaggggcgt gaggcggtac acgactacta
     1681 cgaggacctg cttacttcgt tgcctgacct ccacatcgac attacgcact gcctgggggc
     1741 cgacgagggc gtcgcgctgg aggtccgcat cagcggtacc cacctcgctc cctggcgcgg
     1801 acttccaccc accggccgaa gggtgtcgtt tccgctctgc gggatgttca ccctcgacca
     1861 ggaaggaaag ctggccggag ggcgcatcta ctacgaccgg ggaagagtgc tgcgccgggc
     1921 ttcctaactc gccctgttgc cgatcgacgc gttgcgctcg gccacctgat cagtatggtt
     1981 tgtgaatgcg ctggataact gagggggatc tatgaccttc aagccggcga tctggtatcc
     2041 gatcgcagcc ggactgagcg tcctaaacgt ggtcgccgtc ggatttgcag ccgccgcggc
     2101 cgagccttgg cacgccggca ttcatgcgac gctcgggcta gcgttcgggt tatgggctca
     2161 gcgcttgcgc agaagctaca gcggaagcga gaatgtgctc gaacctagaa tcgaagagct
     2221 cgagatcgag atgggcaggc tgcgccagga gcttaccgaa acccaggaac gactggattt
     2281 cgccgaacgg gtactggcgc agccacagga aacccgccga gtgggtccgc aaccctagcg
     2341 gagcggtcac tggatattca agtagccgcg tctgccgacc tctcggcgca gacccttgac
     2401 gacatccttg cgctctgcga gcgggcgtac ggcgaagatc tgcggccgag cttcctcaca
     2461 ttcaccgacc cgactcacgt catggccttc gccgatggag ccgtgctgag ccacgccatg
     2521 tgggtcacac gttggctggc accaggcgcc agtcctccgc ttcgagctgc atacgtcgag
     2581 gccgttgcca ccgatccggc gtaccaaggt cgtggtctcg ccacggaggt catgcgcgtt
     2641 ctggtgagcc gactgtccag ctttcagctt gcggcactct gcccctccga tcgaggacaa
     2701 tcgttgtatg agcgtctcgg atgggagccg tggagggggc cgctcttcat ccgccaggag
     2761 aggcatctga tcgagacgcc tgatgaggcg gtgatgatac atcggctgcc ggcatcaccg
     2821 ccgctcaacc tggacgaccc cttgtccgcc gagtggaggc caggggagtt gtggtagaca
     2881 gttccatgcg agatccggcg tcctcaacgg agct
//
LOCUS       DASRJQ010000489         3803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3052435, whole genome shotgun sequence.
ACCESSION   DASRJQ010000489 DASRJQ010000000
VERSION     DASRJQ010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3052435"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..1259
                     /locus_tag="VFB89_11210"
     CDS             234..1259
                     /locus_tag="VFB89_11210"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZM27918.1"
                     /translation="MTSTEQQSGAHVGPDGTFRNPWPNSEPHGLKALLRWAFQRRVQT
                     LAATPRKGSFAAASPAILYPRGSTADFTATWIGHSTVLLQIGGLNVLTDPVFSERAFP
                     VQWIGPRRLMNPGLAIEALPPLDTILLSHNHYDHLDRPAVKRIAEAHPGATWIVPLGL
                     GAYIRAWGAREIIELGWWQDTVVNDLHATATPARHFSNRRLGDRNRSLWCGFALELDG
                     KRAYFAGDTGYHPEFSEIGARCGPFDFVMIPIGAYDPRWFMERLHVDPEEAVRIYQDL
                     IAPHPSAPHPLMLGIHWGTFRLTDEPMEEPPRRTVARWREAGLEEGRLWIARFGETRP
                     VGGTGTS"
     gene            complement(1323..2465)
                     /locus_tag="VFB89_11215"
     CDS             complement(1323..2465)
                     /locus_tag="VFB89_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27919.1"
                     /translation="MLTHDDILAALEQEFPRSAQQPPDGVFLVARLQEPGLAMSDTTP
                     YVFIPDIHLVPEVDAERFPWVTARDGQVDALERLARVLGRLRSSDPTLRVWQLGDCLD
                     LWRTGEVGGAVADDVDATVEDRHEVIDALCNDAGMLLLAGNHDQELLDFRWAGGPQAL
                     NTVILNRGTSAADTVLAHGHQFDPIESLSRSFKEFFARGATERVPPTPRSMLEATNPH
                     WKPEAPDAPPPKRPGDRNSFLYYDLRRANPLPLNKVQVNVVPYTPVQDPARAFIDSFG
                     GAARKPTVDGPRQTFFTDIAWYAQQISHTGGKDVRLVVIGHTHHARIVRGTRPDGTLF
                     VLMDCGAWVDTSFLSNELDGPMANGQIGVKVGDDLRIYQLGYTLRT"
     gene            complement(2517..>3803)
                     /locus_tag="VFB89_11220"
     CDS             complement(2517..>3803)
                     /locus_tag="VFB89_11220"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HZM27920.1"
                     /translation="ATINVLQPRVVGGGPFVNNAILLEMAESTWNVWATKYDFPLPWQ
                     PIADRMQIVAHDLGRTPPGTAAGQRSVFFRQGATQLGQTAADLPLAVWNCLGCGGCNV
                     GCRFGRKTGGLHGPRPAGAPRSYLQRALAAGAVLRPEIEAVRFSRAGFLSNRVGALVA
                     HDLAAGGTEVKIRARAFAVTAGPIASSQILARSLPGTDCPPGTNISANVVTPLFAVGA
                     TAFGGNPDPGIQMCYFVDPGGNLLIESWFHYPASLAIAIPGWLDEHAATMRSYGKLAS
                     AGVVVASKPSGRLGLLTDLVLELDNSELDRLKQGVAQLAAIYFAAGAETVIPSTTEPL
                     PIRRAQQQADTTRFMAQVTSAAQLNLNTAHPQGGNRIGRHSGSSVVDPTFKVHDVDNL
                     FVADASVFPAGCGVNPQLTVMALASLAADQIAHKLG"
BASE COUNT          654 a         1245 c         1248 g          656 t
ORIGIN      
        1 ttcgcctgga ggggaagttc gcagcgttga cggatgaccg tgcgggcgcg atccatgcct
       61 accatcacta ccttgcgctg cgcgatgatc ccggcccatc gcttcgtccc cagcgcaaca
      121 gcgtgctcgc cgaccttcac gccctcgagc gctgaaattc cccgcggcga tctccttgcc
      181 taggcagcgt ccacgcggtt gacttcgaca aagcctgcga cccccgggtg ggcatgactt
      241 ccactgagca gcaatctggt gcgcacgtcg gacccgacgg cacatttcgc aacccgtggc
      301 cgaacagcga accgcacggc ctgaaggccc tcctccgatg ggcgtttcag cgacgtgtcc
      361 aaacactggc ggcgacgccg cggaaaggca gcttcgctgc tgcgtcgccc gcgatcctct
      421 atccccgagg aagcaccgcc gatttcaccg ccacctggat cgggcattcc accgtgctgc
      481 tgcaaattgg cgggctgaac gtgctgaccg acccggtgtt cagtgagcgc gcctttccgg
      541 ttcagtggat agggccccga cgcctcatga atccggggct cgcgatcgag gcgcttccac
      601 cgctcgacac catcctgctg tcgcacaacc actacgatca cctggaccgg ccggccgtga
      661 agcggatcgc ggaggcacat ccgggggcga cctggattgt gccgcttggg cttggggcat
      721 acatccgcgc ttggggcgcg cgggagatta tcgagctggg ctggtggcag gacacggtgg
      781 tcaacgacct gcatgctacg gccactccag cgcggcactt cagcaatcgg cggctcggcg
      841 atcgaaaccg gtcgctgtgg tgcggattcg cgctcgagct tgatgggaag cgtgcttact
      901 tcgccggcga caccggctat caccccgagt tcagtgagat tggagctcga tgcgggccat
      961 tcgactttgt catgattcca attggggctt acgatccgcg ttggttcatg gaacgcctcc
     1021 atgtggaccc ggaggaggcg gtgcgaatct accaggactt gatcgctccg catccgtcgg
     1081 cgccccaccc tctcatgctc ggaatccact ggggaacgtt tcgactgacg gatgagccga
     1141 tggaggagcc gccgcgaagg acggtcgccc gctggcggga ggccgggctg gaagaaggcc
     1201 ggctctggat cgcgcgcttt ggtgagacgc ggcccgttgg cggaaccgga acctcgtgag
     1261 tcgcgctcgc cgaacggata catgaagcga ggcgcgagga ttattcgagc aggccaaggg
     1321 gctcaggtgc gaagcgtgta ccccagctgg tagatccgca gatcgtctcc gaccttgacg
     1381 ccgatctgcc cgtttgccat cggcccatcc agctcgttcg acaagaagct cgtgtccacc
     1441 caggctccgc agtccatcag cacaaacagc gttccgtcgg gtcgcgtgcc ccgcacgatg
     1501 cgggcatgat gggtgtggcc gatgacgacg agccgcacat ccttgccgcc ggtgtgcgag
     1561 atctgctgcg cgtaccaggc gatgtcggtg aagaacgtct gccgcggccc gtccacagtc
     1621 ggcttgcgag ctgcgcctcc gaacgaatcg atgaatgccc ttgccgggtc ctgaaccgga
     1681 gtatagggca cgacattgac ctgcaccttg ttcaggggaa gagggttggc gcgccgcaga
     1741 tcgtaataca ggaagctgtt ccggtccccc gggcgcttgg gcggcggcgc gtccggcgcc
     1801 tccggcttcc agtgcgggtt agtcgcttcc agcatgctgc gcggcgtggg cgggacccgc
     1861 tcggtcgcgc cccgcgcaaa gaattccttg aagctgcgcg agagcgattc gatcggatcg
     1921 aactgatggc catgcgccag cactgtgtcg gctgcgctgg tgccgcgatt gaggatcacg
     1981 gtattgagag cctgcggtcc tccggcccag cgaaagtcga gcagctcctg gtcgtgattg
     2041 ccggcgagca atagcatgcc ggcgtcattg cagagtgcgt cgatcacttc atggcggtct
     2101 tcgacggtgg cgtccacatc gtcggcaacc gcacctccga cctcgccggt acgccagagg
     2161 tcgagacagt cgcccagctg ccagacgcga agggtcggat cgctcgaacg cagccggccg
     2221 aggacccgcg ccaggcgctc gagcgcgtcc acctgtccgt ccctcgcggt gacccaggga
     2281 aagcgctccg catcgacttc gggaacgaga tggatgtcgg ggatgaagac gtagggcgtg
     2341 gtgtcggaca tcgcgagccc tggctcctgg agtcgcgcga ccaggaatac accgtccggc
     2401 ggctgttgcg cgcttcgggg aaactcctgc tccaacgctg cgagaatgtc gtcgtgagtc
     2461 agcatcccac ctcccctggg agcaaagtcc gcggcgaatg catcgactcg gctgcttcag
     2521 ccgagcttgt gtgcgatctg gtcagccgcc aggctcgcga gggccatgac ggtcagttgt
     2581 gggttcacgc cgcagccggc cgggaacacg ctcgcgtcgg cgacaaacag attgtccacg
     2641 tcgtgcacct tgaacgtcgg atccaccacg ctcgatcctg aatggcggcc gatgcgattc
     2701 ccgccctgtg gatgggcagt attgaggttg agctgggccg cgctcgtgac ctgtgccatg
     2761 aatcgcgtcg tgtccgcctg ctgctgggcc cgccggatag gcagcggttc cgtcgtcgaa
     2821 ggaatgacgg tctcggcccc ggcagcgaaa taaatcgccg ccagctgagc cacgccctgc
     2881 ttcaatcggt ccagctcgct gttatccagc tccagcacga gatcggtgag cagccccagc
     2941 cggccgctcg gcttggacgc aaccacgacg ccggccgacg cgagctttcc ataggaacgc
     3001 atggtggcgg cgtgctcgtc gagccaaccg ggaatggcga tcgcgagcga cgccggatag
     3061 tggaaccagc tctcgatcag caggttccct cctgggtcta cgaagtagca catctggatc
     3121 ccgggatcgg gattcccacc gaacgccgtc gcgcccaccg cgaagagcgg cgtcacgaca
     3181 ttggcggaga tgttggtgcc gggcgggcag tcggtgccgg gaaggctgcg cgccagaatt
     3241 tgactgctcg caatcggtcc ggccgtaacc gcaaacgctc gggcgcggat ctttacctct
     3301 gtgccacctg ccgcaagatc gtgggccacc agcgccccca cccggttcga gagaaagcca
     3361 gctcgactga atcgtacagc ttcgatctcg ggccgaagca ctgcgccggc cgcgagcgcg
     3421 cgctggagat agctgcgcgg cgcaccggcg ggccgggggc cgtggaggcc accagttttt
     3481 ctcccgaagc gacacccgac gttgcagccg ccgcagccca gacagttcca caccgcgagc
     3541 ggcaggtccg ccgccgtttg gcccagctga gtcgcgccct gacgaaagaa cacgctgcgt
     3601 tgcccagccg ccgtgcctgg gggagtgcgc ccgagatcat gggccacgat ctgcatccgg
     3661 tccgcaatcg gctgccaggg cagaggaaag tcgtacttgg tggcccaaac gttccacgtg
     3721 ctctccgcca tctcgagcag gatcgcattg ttgacgaacg ggccgccgcc cacgacccgg
     3781 ggctgcagca cgttgatggt ggc
//
LOCUS       DASRJQ010000490         3807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3419787, whole genome shotgun sequence.
ACCESSION   DASRJQ010000490 DASRJQ010000000
VERSION     DASRJQ010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3419787"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..538)
                     /locus_tag="VFB89_11225"
     CDS             complement(26..538)
                     /locus_tag="VFB89_11225"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HZM27921.1"
                     /translation="MNLRATRACWLILQLLGLWYPQVLPAQQRIDCVVSHITDGDTFW
                     ARCPDDVKVRLLLIDAPEQDQFPFGRQAKAAIEKLLPLRSRVALEFDVQSTDRYGRTL
                     AYVFLPDGRMVNEEMAREGYAIALVYPPNVRYVERIRRAVVQACEARRGLWAQGGFAC
                     PPKQHRQKQC"
     gene            735..1055
                     /locus_tag="VFB89_11230"
     CDS             735..1055
                     /locus_tag="VFB89_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27922.1"
                     /translation="MSAPKAEQHRVTIRGRELHFVAYEGIPANPRRGYPAVPPTWYLM
                     SWGKRHKVMEYLIGQPVEERDAALRKWAEENALGPISDEKVRASFRKQTADVRREAWW
                     PASY"
     gene            1071..1952
                     /locus_tag="VFB89_11235"
     CDS             1071..1952
                     /locus_tag="VFB89_11235"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZM27923.1"
                     /translation="MFLLTLALLAGSPDSAVVRDIQVAPGETLRTTTIGSGLPLVLIP
                     GLFGAAFGYRTITGPLVARGCRCIIIEPLGYGWSSRPKRADYSFTAQTERVGRALDQL
                     GVTRALFVAQSSGASIVFRLAIARPELVRGLLSIDGGPAETASTPGLRKAFSHGSFLA
                     KLVFDESMLRSEVRQEIVRNSGDTSWVTDAVIHGYTAGQAADMHGAINAFERMAKSTE
                     PTSLAAQLHDCAAPVLLLVGTVPHPAGIGAEQRELLKAKLRHFKTESVAGSGQYIQEE
                     QPAAVVAAVSRLDKTAP"
     gene            2135..2347
                     /locus_tag="VFB89_11240"
     CDS             2135..2347
                     /locus_tag="VFB89_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HZM27924.1"
                     /translation="MRTTGTVKWFNDAKGFGFITPENGEKDCFVHHSAIQAQGFKSLT
                     EGERVEFDIVQGQKGPAAENVTKIGQ"
     gene            2453..2653
                     /gene="rpsU"
                     /locus_tag="VFB89_11245"
     CDS             2453..2653
                     /gene="rpsU"
                     /locus_tag="VFB89_11245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HZM27925.1"
                     /translation="MTEVRLNEGDSVDYALKLFRRKLQKTGLFQELRRRRFYVKPSAA
                     RLLKSTAARRRKRKRARAHDSW"
     gene            complement(2673..>3807)
                     /locus_tag="VFB89_11250"
     CDS             complement(2673..>3807)
                     /locus_tag="VFB89_11250"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM27926.1"
                     /translation="ATLRTAAGFYEQAVVLDSTFAQAWAQLARAQTYYYYIGTATPAG
                     AEAARRAAERAAALAPGRAESQLALGDYYEQVKGDNARALEAYQVGLKSEPSNGDLLT
                     ASALAEQSLGRWEAALAHLQQAQTLDPRSVLTARRLGLTLLRLRRYPEALAAGERGVA
                     LAPTNLQMLEYLAMVHLALGDLAGARAVVQSASKEIAPTALVAHFGNYWDLYWMLDDA
                     QQQLLLRLPPSAFDNDRATWGIVLAQTYWVRGDQKRTRAYADSSRIALEEQLSATPDN
                     AQQRIFLGLALAYLGRKADAIREGERAVALLPISKDGYTGPYLQHQLARIYTVVGEQE
                     KALDQLEPLLKVPYHLSPGWLRIDPEFTALKGNPRFEKLRAGA"
BASE COUNT          673 a         1237 c         1252 g          645 t
ORIGIN      
        1 gtatctcttc caagggtcga agacgttagc actgcttttg gcggtgctgc tttggagggc
       61 acgcgaagcc tccctgggcc cagagccctc gccgggcttc gcatgcttgc acgacggctc
      121 tccgaatccg ctcgacgtac cgcacgttgg gcggatagac cagggcaatt gcataaccct
      181 ctcgagccat ctcctcgttc accatccggc cgtcaggcag aaagacatac gcgagcgtcc
      241 ggccatatcg gtcggtcgac tggacatcga actccaaagc gactcggctc ctcagcggca
      301 ggagcttttc gatcgccgcc ttggcttggc ggccgaaagg aaattgatcc tgctctggag
      361 cgtcgatcag gagcaaccga actttgacgt catcggggca acgggcccag aaggtgtccc
      421 cgtcggtgat gtgcgagacg acgcagtcga tccgctgctg ggcgggtagc acctgcgggt
      481 accacaagcc aagcagttgc aggatgagcc agcaggctct cgtcgcgcgc aagttcatgc
      541 ggctccgtgc tatgctgggc aattgaaacg acatagtgac gtaacgaaca ggggacgtga
      601 gttgcctgcg tcaccgcgca agagcgtccg ccaggttatc tttagcacgt tgggtccatg
      661 gagtacccga ccgcgcctgc ctccgtctga tgaggagact cgtgtgagcc tccccctctc
      721 ccatcgagac ctccgtgtcc gcccctaaag ccgagcagca ccgcgtcacg atccgcggtc
      781 gcgagcttca ctttgtcgcc tacgaaggca tccccgcgaa cccccgtcgc ggataccccg
      841 ccgtgccgcc cacctggtac ctcatgagct gggggaaacg gcacaaggta atggagtatc
      901 tgatcggcca gccggtcgaa gagcgagacg cagcgttgcg aaaatgggcc gaggaaaacg
      961 cgctcgggcc catcagcgat gagaaggtcc gcgccagctt ccgcaagcag acggccgacg
     1021 tccgacggga agcctggtgg ccggctagct actaaaggaa ggagaatccc atgtttctcc
     1081 tcaccctggc gctcctcgcc ggttcgccag acagcgccgt cgttcgcgac atccaagtgg
     1141 cgcccgggga aacgctgcga accaccacga taggctcggg gctgccgctg gtcctcatcc
     1201 ccggcctctt cggcgccgcc ttcggctatc gcaccattac cggtccgctg gtcgctcgcg
     1261 gctgtcggtg catcatcatc gagccgctcg gctacggctg gtcgtcgcgc cccaaacgcg
     1321 ccgactactc cttcactgca cagaccgagc gcgtcggccg agcgctcgat cagctggggg
     1381 tcacgcgagc gttattcgtg gcacagtcca gcggcgcctc cattgtgttc cgtctcgcca
     1441 ttgcccgccc cgagctggtt cgcggactgc tgtcgatcga cgggggcccg gccgagacgg
     1501 cgtcgactcc gggcctgcga aaggcgttca gccacggcag ctttctggca aagctcgtgt
     1561 tcgacgaatc gatgctccgg agtgaggtgc ggcaggaaat cgtccgcaat tctggggata
     1621 ccagctgggt gaccgacgcc gtgatccatg ggtacaccgc agggcaggcc gccgacatgc
     1681 acggcgcgat caacgcgttc gaacgaatgg cgaaatcgac ggagcccaca tcgctcgccg
     1741 cccagctgca cgactgcgcg gcgccggtgt tactgctggt gggcacggtg ccgcatccag
     1801 cggggatcgg cgctgaacag cgggagctgc tgaaggcgaa gctccggcac ttcaagaccg
     1861 agagcgtggc cggttcgggc cagtacatcc aggaggaaca accggcggct gtagtcgcgg
     1921 cggtcagccg gctggataag acggcgcctt gatagcgctt acgaatccgc tgctcggagg
     1981 ttatattgtc agcctgtgag tgtgcgatgc acccacatcc gacgtgaatt gaggggctgg
     2041 cgttggcgcc ggcccctttt tcatgtccca agcgtagtcg gccggtacgg cgtatgtccg
     2101 cactccgatt acacaccacc aaggagtagg aacaatgcgt acgaccggca cggtgaagtg
     2161 gttcaacgac gccaagggct ttggcttcat tacgcccgaa aacggtgaga aggactgctt
     2221 cgtccatcat tcggcaattc aggctcaagg tttcaagagc ctgactgaag gtgagcgggt
     2281 ggagttcgac atcgtgcagg gccagaaggg cccggcggcg gagaacgtca ccaagatcgg
     2341 gcagtaaccc gattacgacc cccgcgcagt aacggcggcg gccggttcgc ttggtgcggg
     2401 ccggccgccg ctacttcgga gcgctgatga gcgctcgagc aaggatgact gaatgaccga
     2461 agtcaggttg aacgaggggg acagcgtgga ctatgcgctc aaactcttcc ggcgcaagct
     2521 gcagaagacg gggctcttcc aggagctccg ccggcggcgg ttctacgtca agccaagcgc
     2581 ggctcggctg ctcaagtcga ccgcggcgcg gcggcgcaag cggaagcggg cccgcgcgca
     2641 cgactcctgg tagccacccg caggaaaaat cctcacgccc ccgcgcgtag tttctcgaac
     2701 cgcgggttgc ccttgagcgc tgtgaattca gggtcgatgc ggagccagcc gggtgatagg
     2761 tgatagggga ccttgagcaa gggctcgagc tggtccagcg ccttctcctg ttcgccgacc
     2821 acggtgtaga tccgagcgag ctggtgttgg aggtacgggc cggtgtaccc gtctttgctg
     2881 atcggcagca gcgccaccgc gcgctccccc tcgcggatgg cgtccgcctt ccggccgagg
     2941 tacgccaacg ccagtccgag aaagatgcgc tgctgggcgt tgtccggcgt cgcgctgagc
     3001 tgctcctcca gcgcgatgcg gctggaatcg gcgtaggcgc gcgtgcgttt ctggtcgccg
     3061 cgaacccagt aggtctgggc cagcacgatc ccccaggtcg cccggtcatt gtcgaacgcg
     3121 ctcggcggga gcctgagcag aagctgctgc tgcgcatcgt ccagcatcca gtagaggtcc
     3181 cagtagttgc cgaagtgcgc gacaagtgcg gtcggcgcga tctccttgga ggcactctgc
     3241 acgacggccc gcgcccccgc gagatcaccg agcgcgaggt ggaccatcgc cagatactcg
     3301 agcatctgca gattggtcgg cgcgagcgcc acgccgcgct cgcccgctgc cagagcctcg
     3361 ggatagcgac gcagccggag tagcgtaagc cccaaccggc gcgcagtgag caccgatcgg
     3421 gggtcgagcg tttgcgcctg ctgcaaatgc gccagcgcgg cctcccaccg gcccaggctc
     3481 tgctcggcca gcgccgaggc ggtgagcagg tcgccgttcg acggctcact cttgagcccg
     3541 acttggtagg cctcgagcgc ccgggcgtta tcgcccttca cttgttcgta gtagtcgccc
     3601 aacgcgagct gactctcggc acggccaggc gcgagcgccg cggcgcgctc ggccgctcgc
     3661 cgagctgcct ccgcccccgc cggcgtggcg gtgccgatgt agtagtagta ggtctgcgcc
     3721 cgggccagct gcgcccaggc ctgggcgaac gtcgaatcga gcaccaccgc ctgctcgtag
     3781 aacccggcag cggttcggag agtcgcg
//
LOCUS       DASRJQ010000491         6387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_308684, whole genome shotgun sequence.
ACCESSION   DASRJQ010000491 DASRJQ010000000
VERSION     DASRJQ010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308684"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VFB89_11255"
     CDS             <1..467
                     /locus_tag="VFB89_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27927.1"
                     /translation="GVVYNGTRGALNSLGTSKESGDLRYKTVTFGQNFLAGPVAGPGA
                     GTPVVLDQGWFQNYYTTFTFLGVPFYESASFTKLREVSIGYSFTGGFISRSLGFSSVD
                     LRAAGRNLFVWTDYTGVDPETNLAGAETGARNIDWFNNPQNRSFVFTVTLNR"
     gene            497..1834
                     /locus_tag="VFB89_11260"
     CDS             497..1834
                     /locus_tag="VFB89_11260"
                     /inference="COORDINATES: protein motif:HMM:NF024181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HZM27928.1"
                     /translation="MRKHLAAIALALGLGLGSSACSDFLTGPKLSDNPNRPTVANNAN
                     LLVSAETNLSFQSESHLARTICIWMQQCAGTNKQYLSLGLYITGDDDYYYDGWIDAYT
                     RGGLLDLRRIQHDALQVADSAYAGVAYVLEAWLMGQTSDVWGDIPYSQAADSTVAHPA
                     PDPQQRVYETVQAKLDTAIIFLNTTTAPPGGEDLVYEGDVTKWAALANTLKARFYLHT
                     AERDPTAYQKAFTAASSGITDATGAGDYLAYHSSAVTTENNIWHQFVAVSWPQYLSAG
                     KFLVDTLNALADPRISQYFAENSDGVFVGAAPGQNVGVNPSELSETRLDAGFRQPFVT
                     YVENELILAEAAFRIGDASAGTHLNNARTAQGLTALVTVDLPTIMLEKYIALFQNMEV
                     WNDYKRTCLPDLTPATGASQIPVRLVYPLSERTANPSISGAGPARNWNDPNPC"
     gene            complement(1908..3986)
                     /locus_tag="VFB89_11265"
     CDS             complement(1908..3986)
                     /locus_tag="VFB89_11265"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZM27929.1"
                     /translation="MSFSRMRRGHLARAALPIVALAFPLSPTFPQAPARRAFSLDDLA
                     RIRTITEPAISPDGAWVSYTVTTVDSARDREQSDIWMTSWDGTRTVRLTSSPESEHGA
                     AWSPDGRWLAFLSDRGDPHENDQLWLLDRAGGEAERITGFPGGIDDFAWAPDGKRLAL
                     VVEDPERDTVSADTGSAKGAKRPLPIVLDRFQFKEDETGYLDQRRKHLYLLDVATRRG
                     QQLTSGTHDEVLPSWSPDGSTIAFVTKRGADPDRTDNWDIYTVAAEPGGAERQVTTYE
                     GADSDPDLASRPAWSPDGKAIAYTQGGPPKLIDYGVPHLAVVPAVGGAAKPPAPALDR
                     WVKEPRWASSGRDLLFLLEDDRNVQLARVPAEGGSVERVVTGRRVLSDIAVGPDDRVA
                     VLAATPDAPAEVFAVERDGSLRALSHQNDDWLKEISLATTDEISFRSRDGTQINGLLV
                     KPPGWAPGSRYPTVLRIHGGPVLEVENEFNEQWQWLASRGYVVIAVNPRGSSGRGEKF
                     ASAIFADWGHKDAQDVLAAVDYAVREGIADPNRLGIGGWSYGGMLTNYVIAQDQRFKA
                     AISGAGISNILAGYGTDQYVREYETELGLPWKHTDRWLKLSFPFLHADRIVTPTLFLC
                     GDRDFNVPLLNSEQMYQALRSLGRETELVIYPGEYHTIRRPSFVRDRLKRYAEWYDRF
                     LKQGTLNAEQ"
     gene            complement(3983..5308)
                     /locus_tag="VFB89_11270"
     CDS             complement(3983..5308)
                     /locus_tag="VFB89_11270"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZM27930.1"
                     /translation="MRLALLLAGAAGAALLCPATARSQQLIAVHAGRLIDVDRGEVAR
                     DRLILIRGDSIAAVEPWPAKPPAGAKVVDLSRATVAPGLIDCHTHLVDDIQSADVLAS
                     LKRSGAQQVMIGVRQARATLLAGFTTVRDVGTYRAFTDVALRDAINDGTIIGPRMAVA
                     GAYITVSTGGGEVTGMAPDITLPAEFRMGVANSADEIREKVRILIHGGADLIKVIATG
                     AVLTSGTNPGAPEFTEAEIHAAVDEAAKYGVFVAAHAHGAEGIKAAVTAGVRSIEHGS
                     LIDDEGIRLMKEKGTYLVADIYNGDYIATVGKQQGWSAEILRKNDETTEAQRQGFKKA
                     VAAGVKIAFGTDAGVYPHGDNARQLPYMVRWGMTPMQAVQSATIVAARLIGWDSRVGS
                     VAPGKYADLVAVDGDPLANLESFTKVTFVMKNGVIYKMAGQPVGRAVPE"
     gene            complement(5420..>6387)
                     /locus_tag="VFB89_11275"
     CDS             complement(5420..>6387)
                     /locus_tag="VFB89_11275"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HZM27931.1"
                     /translation="ADLADDFADATWHSETLCINAHGVAKFALSRAVRDAGYKVVLTG
                     EGSDEILAGYPHFRRDMLLYDNKGQDPGVIRTLLDQLDRNNQVSRGLLLPDGEAASLA
                     QVQRVLGFAPTWMEAAAGRMFKMQAIWADDFQAEFAGQEAYHGLLGSLDLPGQLAGRA
                     PVNQALYLWAKTLLPGYILTVLGDRMEMAHSIEGRVPFLDHEVVELICSMPVEQKIRG
                     MTEKFVLREAARPVLTDTVYQRQKHPFLSPPATLNPKDRLHEMVQDTLRGQGIRYVPF
                     VDQARVVRMLDGLPNLDPGEQVSIDQILMVLLSASVLGERFALAA"
BASE COUNT         1162 a         2060 c         2032 g         1133 t
ORIGIN      
        1 gcggagtggt ctacaacgga actcgtgggg cgctcaactc actcggcacg tccaaggagt
       61 ctggcgacct gcggtacaag acggtgacgt tcgggcagaa cttcctggcc gggccggtgg
      121 ccggacccgg agcgggaacg ccggtcgtgc tcgaccaagg atggttccag aactactaca
      181 ccactttcac gttcctcggc gtgccgtttt acgagagcgc cagcttcacc aagctgcgtg
      241 aggtctcgat cggctattcg ttcaccggcg ggttcatcag ccgcagcctc gggttcagca
      301 gcgtggacct tcgtgccgcc ggccggaacc tgttcgtgtg gaccgattac accggggtag
      361 atccggaaac gaatctggcg ggcgccgaaa ccggcgcgcg gaacatcgac tggttcaaca
      421 acccgcagaa ccgctcgttc gtctttacgg tgaccctgaa ccgctgaccg aaccccgacg
      481 actcgaggat cacgatatgc gtaagcatct tgccgcgatc gcgctcgcgc tcggcctggg
      541 gcttggcagc agcgcctgca gcgatttcct gaccgggccg aagctgtcgg acaaccccaa
      601 ccggccgacg gtcgcgaaca acgccaattt gctggtttcg gcagaaacga atctctcgtt
      661 ccagtccgag agccacctgg ctcggacgat atgtatctgg atgcagcagt gcgccggcac
      721 caacaaacag tatctgtcac tcggactcta tatcacggga gatgacgact actactacga
      781 cggctggatt gatgcgtata cgcgaggcgg tctgctcgat ctgcgccgga ttcagcacga
      841 cgcgctgcag gtcgccgact cggcgtatgc cggggtggcc tacgtgctcg aggcctggct
      901 catgggtcaa acgtcggacg tgtggggcga catcccgtac tcgcaagccg cggattcgac
      961 cgtcgcccat ccggcgcccg atccccaaca gcgggtgtat gagacggtgc aggccaagct
     1021 cgacaccgca atcatcttct tgaatacgac gaccgcgcct cccggtggag aggaccttgt
     1081 ctacgagggc gatgtcacca agtgggctgc gctggccaat acgctcaagg cgcgcttcta
     1141 cctgcatacc gcggagcgtg acccgacggc gtatcagaaa gcgtttacgg ccgcttcgtc
     1201 gggaatcacc gatgcgaccg gggccggtga ttacctcgcc tatcacagca gtgccgtcac
     1261 cactgagaac aacatctggc accagtttgt ggcggtgagc tggccccagt atctctcagc
     1321 cggcaagttc ctggtggaca cgctcaacgc cttggccgat ccgcggattt cccaatactt
     1381 cgcggagaac tccgacggtg ttttcgtcgg agcggcgcca ggacagaacg tgggcgtcaa
     1441 cccctcagag ctcagcgaga cccggctcga cgctgggttc cgtcagccat tcgtgaccta
     1501 cgtagagaac gagctgattc tggcggaagc ggcgttcaga atcggcgacg cctccgccgg
     1561 aacgcatttg aacaacgcaa ggaccgctca ggggctcaca gcccttgtga ctgttgatct
     1621 tccgacaatc atgctcgaga agtacatcgc gctgttccag aacatggagg tctggaacga
     1681 ctacaagcgg acctgcttgc cggacctcac gccggccacc ggagcaagtc agatcccggt
     1741 caggctggtg tatcccctga gcgagcggac ggccaacccg tcgatttcgg gagcggggcc
     1801 ggcgagaaac tggaatgatc cgaatccgtg ctagaaacag cgtgaacggt aaacagtgaa
     1861 cggtgaacgg gggctccggc tcatcagcgg agccccccgt tcactggtta ctgctcagca
     1921 ttcagggttc cctgtttcaa gaaccgatcg taccactccg cgtaacgctt cagcctatct
     1981 cgcacaaagc tcggccgccg gatggtgtgg tactctcccg gatagatcac cagctcggtc
     2041 tcgcgcccaa ggcttctgag cgcctggtac atctgttccg aattgagcag cggcacgttg
     2101 aaatcgcgat cgccacagag gaaaagagtc ggcgtgacga tgcgatcggc gtgcaggaac
     2161 gggaaggata gtttgagcca ccggtcggtg tgcttccacg ggagacccag ctcggtctcg
     2221 tattctcgca cgtactggtc ggtaccgtag cccgcgagaa tgttcgagat tcctgccccg
     2281 ctgattgcgg ccttgaatcg ctgatcctgc gcgatgacgt agttggtcaa catcccgccg
     2341 tagctccagc ccccaatgcc gagccgattc ggatccgcga tgccttcgcg tacggcgtaa
     2401 tccacggccg cgagcacgtc ctgggcgtcc ttgtggcccc agtcggcgaa tatggcggac
     2461 gcgaacttct caccgcgacc cgagctgccg cgcgggttga ccgcgatgac gacatagccg
     2521 cgactcgcga gccactgcca ctgctcattg aactcgttct cgacttccag gactggccca
     2581 ccgtgaatgc gcaggaccgt cggatagcgg ctgcccggag cccagcccgg cggcttcacc
     2641 agcagtccat tgatctgggt accgtcccgg ctacggaatg agatctcgtc ggtcgtcgcg
     2701 agagaaatct ccttcagcca gtcgtcgttc tggtggctga gcgctcgcaa tgagccgtcg
     2761 cgctcgacgg cgaagacctc cgctggtgca tcgggtgtgg ccgccaggac cgcgacgcga
     2821 tcgtccgggc cgaccgcgat gtcgctcagc acgcgtcggc cggttacgac ccgctcgacc
     2881 gagcctccct cggcgggcac gcgggcaagc tggacgtttc ggtcgtcttc caacaggaac
     2941 agcagatcgc gaccggaaga agcccagcgt ggctctttca cccagcgatc cagcgccggc
     3001 gccggcggct tggcggcgcc acccacggcc ggcacgacgg ccaggtgagg aacgccgtaa
     3061 tcgatgagct tgggagggcc gccctgcgta tacgcgatgg cctttccatc cgggctccag
     3121 gcgggacgac tcgccagatc cggatctgag tccgctccct cgtaggtggt gacttgtcgc
     3181 tcagctcctc cgggctcggc cgcaacggtg tagatgtccc agttgtcggt gcggtcggga
     3241 tcggcgccac gcttagtcac gaacgcgatg gtgctcccgt ctggcgacca cgatggcaag
     3301 acttcgtcgt gagtgccgga ggtgagctgc tggccccggc gagtggcgac gtcgagcaga
     3361 taaaggtgct tccgccgctg gtcgagatag ccggtctcgt cctctttgaa ctgaaaccga
     3421 tcgagcacga tgggcagtgg gcgctttgcg cctttggccg agccggtgtc ggcggaaacg
     3481 gtgtcgcgct caggatcttc gaccaccagc gccagccgct ttccatcggg ggcccaggcg
     3541 aagtcgtcga taccaccggg aaagcctgtg atgcgctccg cctcgccgcc ggcccggtcg
     3601 aggagccaga gctgatcgtt ttcgtgcggg tctccgcggt ccgagagaaa cgcgagccat
     3661 cgcccgtcgg gactccaggc agcgccatgc tccgactcgg gagaagaggt gagccgcacg
     3721 gtgcgggtgc cgtcccagct cgtcatccag atgtcgcttt gctcccgatc gcgcgcactg
     3781 tcgaccgtgg tgaccgtata gctcacccac gcaccgtccg gtgagatcgc gggctcggtg
     3841 atggtccgaa tgcgagccag atcgtcgaga gaaaaggcgc gccgggcggg agcttgggga
     3901 aaagtcgggg agagcggaaa ggccagcgcg acgatcggca gtgcggcgcg ggccaagtgg
     3961 ccccggcgca tccgtgagaa gctcattcag gcaccgcccg accgacgggc tgtccggcca
     4021 tcttgtagat cacgccgttc ttcatgacga acgtcacctt ggtgaaactc tccaggttcg
     4081 cgagcgggtc gccgtccaca gcgacgagat cggcgtactt gcccggcgca acggagccga
     4141 cccgagaatc ccacccgatg agacgggcag ccacgatcgt ggcggactga actgcctgca
     4201 tgggagtcat tccccagcgc accatgtagg gcagctgccg tgcgttgtcg ccatgaggat
     4261 agaccccggc gtctgtgccg aacgcaatct tcaccccggc agccaccgct ttcttgaatc
     4321 cctgacgctg tgcttccgtc gtctcgtcgt tcttccgcag aatctccgcc gaccaaccct
     4381 gctgcttacc tacggtcgcg atgtagtcgc cgttgtagat gtcggccacg agataggtgc
     4441 ccttctcctt catgagccgg atgccttcgt cgtcgatgag cgagccgtgc tcgatcgagc
     4501 gaacccccgc cgtcacggca gccttgatgc cttcggcacc gtgcgcgtga gcggccacga
     4561 agacaccgta cttcgcggct tcgtccaccg cggcatgaat ttctgcctcg gtgaactccg
     4621 gagcaccggg gttggtgccg ctggtcagca cggcaccggt ggcgatgacc ttaatcagat
     4681 cagcaccccc atggatgagg atgcgcacct tttccctgat ctcgtccgct gagttggcaa
     4741 cgcccatgcg gaactctgcc gggagggtga tatccggcgc catgccggtc acctcgccac
     4801 cgccggtgct taccgtgata taggcgcctg cgacggccat tcgagggccg atgatggtgc
     4861 cgtcgttgat cgcgtcccgc agggctacgt ccgtgaatgc ccggtaggtg cccacatcgc
     4921 gcacagtggt gaagcccgca agcagggtgg cccgtgcctg cctgacgcct atcatgactt
     4981 gctgcgcgcc cgagcgcttg agcgaggcga ggacgtcggc ggactgaatg tcgtccacca
     5041 ggtgggtatg gcagtcgatc agcccaggcg cgactgtggc gcgggaaaga tcgacaactt
     5101 tggcaccggc cggaggcttg gccggccacg gctcgactgc ggcaatgcta tcgccgcgaa
     5161 tcaggatcaa gcggtcccgc gccacctcgc cacggtccac atcgatcagg cggccggcgt
     5221 gtaccgcaat cagctgctgg gagcgggccg tcgcgggaca gagaagcgcc gcgccggcgg
     5281 ctccggcgag caacaatgca agtctcatcg agaggtcgga gtttcgggtg cgcggaatat
     5341 gcgagcaccg gcgtcgccgc aacaagcccg gcggctccgg tggcaggaag tattaaatga
     5401 accgacgact gcagataact caggccgcca gagcgaaccg ctccccgagc acgctggcgc
     5461 tcaacaacac catcagaatc tgatcgatcg acacctgctc gccgggatcg aggttgggca
     5521 gtccatccag catgcgcacc accctggcct gatccacaaa gggcacgtag cggatacctt
     5581 ggccgcgcag cgtgtcctgc accatctcgt ggaggcggtc cttcgggttg agcgtcgcgg
     5641 gcgggctcag gaatggatgt ttctgccgct gatagaccgt gtcggtcagg accgggcggg
     5701 ccgcctcgcg cagcacaaac ttctcggtca tgccgcggat cttctgctcc accggcatcg
     5761 aacagatgag ctccaccacc tcgtgatcga ggaacggaac gcgtccttcg atcgaatggg
     5821 ccatctccat ccggtcgccc agcaccgtga ggatgtagcc gggcaacagg gtcttggccc
     5881 agagatagag cgcctggttt accggggcac gcccggccag ctgtcccggc agatcgaggc
     5941 tgccgagcaa gccgtgatag gcttcctgtc ccgcgaactc ggcctggaag tcgtcggccc
     6001 agatcgcctg catcttgaac atccgcccgg cggcagcctc catccaggtc ggcgcaaagc
     6061 cgagaacacg ctgcacctgg gcgagcgagg cggcctcgcc gtccggcagg aggagcccgc
     6121 gggagacttg attgttgcgg tccagctgat ccagcagggt gcggatcacg cccggatcct
     6181 ggcccttgtt gtcatagagc agcatgtcgc gccggaagtg cggatagccg gcgagaatct
     6241 catccgagcc ttcgccggtg agcaccactt tgtatccggc gtcgcgcacg gctcggctca
     6301 aggcaaactt ggcgacgccg tgcgcgttga tgcagagcgt ctccgaatgc caggtcgcgt
     6361 ccgcgaagtc gtcggccagg tccgcct
//
LOCUS       DASRJQ010000492         4182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1153427, whole genome shotgun sequence.
ACCESSION   DASRJQ010000492 DASRJQ010000000
VERSION     DASRJQ010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153427"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="VFB89_11280"
     CDS             <1..988
                     /locus_tag="VFB89_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZM27932.1"
                     /translation="GGGLVLQGRVSVGAFIAFGVYLAMLVWPMIALGWAINLIQRGAA
                     AMARINQLLREQPSIVSPANPAAFPPPRGARALAVEGVWFKYPNAAERGWVLQDISFE
                     VTPGRSLAIVGATGAGKTSLVDLLVRTYDPDRGRILVDGVDIRSVSLADLHRAIGFVP
                     QETFLFSQTLRDNVLLGAPDDGRLERVADTSQLAAALSDLPSGFDTMLGERGINLSGG
                     QKQRAAIARALAQDPPIFVLDDALSAVDAQTEAKILAGLRGALAGRTSVIVSHRLTAV
                     READWILVLDGGRLVEQGTHEELVARGSRYWELLRRQQLEEELETANTIGTA"
     gene            complement(1071..1799)
                     /locus_tag="VFB89_11285"
     CDS             complement(1071..1799)
                     /locus_tag="VFB89_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HZM27933.1"
                     /translation="MSEPGSLGFLVAFAAGLLSFLSPCVLPLVPSYVGFLTGMTLPEL
                     SDRRRVAALHALLFVAGFSLIFVLLGASATALGGALKYYQQWIARIGGVLIILFGLIC
                     LGVLRIGFLEQERRVQFQHKPVGYLGSALVGMAFGAGWTPCIGPVLGGILSLAATTES
                     VSRGMLLLGVYSAGLAVPFLIAAMAVESFLDWFQRFRRFLPWVMRVSGALLVGVGLLM
                     ATGQFTRLAGWLQGLTPDFIRRLI"
     gene            complement(1796..2278)
                     /locus_tag="VFB89_11290"
     CDS             complement(1796..2278)
                     /locus_tag="VFB89_11290"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HZM27934.1"
                     /translation="MARLSKGEGAALLERFRRHLRDHHLPVTRQRDLVAQTVLMSDEH
                     LSVEAIQRLLKDEGEKVGTATIYRTLDVLVRSGLVRAHDFGEGFKRYEPMPQQAHHEH
                     LICLRCGTVVEFQNERLERMLPIIADEHAFQHQRHRVEVYGLCRDCRRRDLGPLAGES
                     "
     gene            complement(2262..2666)
                     /locus_tag="VFB89_11295"
     CDS             complement(2262..2666)
                     /locus_tag="VFB89_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27935.1"
                     /translation="MGSGIGLNRPALAPGVGLGWSRLAEEVARLLPREEIDAVWAFPN
                     IRRDGREWGTAVVARVEGGRRRIYTARYQLVLKGKERGQFEAAVEEVGSGPLETIEEL
                     LREVHKRTDDEQPPVSLNPAVWLAPPTDGTTQ"
     gene            complement(2645..3760)
                     /locus_tag="VFB89_11300"
     CDS             complement(2645..3760)
                     /locus_tag="VFB89_11300"
                     /inference="COORDINATES: protein motif:HMM:NF044507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27936.1"
                     /translation="MSDLAPAQPAASAYHFPDFSKPPFVAAPEARFEPLPADGVLPEG
                     FFSTSNLPTYIKLNGRWIMPARPRMDCVVVRRGNELETIEPRRLRRGDLVAMGEAEDG
                     SQGIVVYAEGFLGGSHGANEFRFMSTEVSRERPVNYEELARRLVEEKRSGGYLVWVVG
                     PALVHSRARADFEWFIANGFVQAVLVGNAVAVHDIEAAIFGTTLGMTSTGAPSQGGHG
                     LHMRAINRVRAAGSIECAVQSGLIKSGIMHALVTHQIPFVLAGSIRDDGPLPGVLTDT
                     LEAQDAMREYTVQATGAIFIATALHAIAVGNMLPAFHTRYGPPAPLMTICVDQTEFVV
                     NKLKDRGTHQAYGVVTNAQDFMHVLRFYVERWAAESA"
     gene            3852..>4182
                     /locus_tag="VFB89_11305"
     CDS             3852..>4182
                     /locus_tag="VFB89_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27937.1"
                     /translation="MPPRSMLRVTLLLAILLLSGAPLAAQGGRPDDGQTILRNMTYLK
                     PLVGTWNAVAEYHQKDGSLAYDVGTYKISYILEGTYLQWYAELHDKGDPSQHHSFMNL
                     VTFNPVTG"
BASE COUNT          787 a         1391 c         1333 g          671 t
ORIGIN      
        1 cggcggcggc ttggtgctcc agggccgcgt gagcgtgggc gcgttcattg cgttcggcgt
       61 gtatctggcg atgctggtgt ggcccatgat tgcgctgggc tgggccatca atctgatcca
      121 gcgcggtgca gccgcgatgg cacgaatcaa ccagctgctg cgggagcagc cgagcatcgt
      181 gtcgccggcc aatccggcgg cgttcccgcc cccgcgaggc gcgcgtgcgc ttgcggtgga
      241 aggcgtctgg ttcaagtatc ccaacgctgc agagcgaggc tgggtgctgc aggacatctc
      301 gttcgaggtg acgcccggga ggtcactcgc cattgtcggc gccaccggag cgggaaagac
      361 gtcgctggtg gacctgctgg ttcgcaccta cgatcccgac cggggacgca ttctggtcga
      421 tggggtcgac atccggagcg tctcactcgc agacttgcac cgcgcgatcg gcttcgtgcc
      481 gcaggaaacc tttctcttca gccagacgct gcgagacaac gtgctgctcg gcgcgccgga
      541 cgatggaaga ctcgagcggg tagcggacac gtcgcagctc gcggctgcgc tgtccgatct
      601 gccgtcgggg ttcgatacca tgctcggcga gcgggggatc aacctgtccg gcggacagaa
      661 gcaacgcgct gccatcgcac gcgccctggc gcaggacccg cccatctttg tgctggacga
      721 tgcgctgagc gcagtcgacg cgcagaccga ggcgaagatt ctcgccggtc tccgtggcgc
      781 gctcgcgggc cgcaccagcg tgatcgtctc ccaccgactc accgcagtgc gcgaggcaga
      841 ctggattctg gtcctcgacg gcggccgcct ggtggaacag ggaacccacg aagagctggt
      901 cgcgcgcggg agtcgctact gggagctgct gcgcaggcag cagttggagg aagaattaga
      961 gaccgcgaac acgatcggaa cggcgtaaac ggtgaaccgt aaacgggggt taccggatcg
     1021 tcggagcccc cgttcactgt tcaccgttta ccgttcacgc tgtttttgtc ttagatcagt
     1081 ctccggataa agtcgggggt cagtccctgc aaccacccag ccagccgggt gaactggccc
     1141 gtcgccatca ggaggccgac cccgaccagc agtgccccgc tcacccgcat cacccacgga
     1201 aggaagcggc gaaaccgctg gaaccagtcg aggaacgatt cgaccgccat cgcggcgatg
     1261 agaaacggaa cagccagtcc cgctgaatag acaccgagga gaagcatgcc gcggctgacg
     1321 ctctcggtgg tcgccgcgag actcaggatc ccacccagca ccggcccgat gcagggagtc
     1381 catcccgcgc cgaaagccat tccaaccagc gccgatccca gataaccgac cggcttgtgc
     1441 tggaactgaa cccggcgctc ctgctccagg aagccgatgc ggagcacgcc gagacagatc
     1501 aggccgaaca gaatgatgag aacgccgccg atgcgggcga tccactgctg gtagtacttg
     1561 agggcgccgc ccaaggccgt cgcactggcg ccgagcagaa cgaagatgag cgagaaaccc
     1621 gcgacgaaaa gcagcgcatg cagggccgcc acccggcggc gatccgacaa ctccggcagc
     1681 gtcatcccgg tgaggaatcc aacgtaggac ggaaccagcg ggagcacgca gggagacagg
     1741 aagctcagta gcccggcggc gaaagccacc aggaaaccca ggctgcccgg ctcactcatg
     1801 attcccccgc gagcgggccg agatcgcggc gacggcaatc ccgacagagt ccgtagactt
     1861 cgacccgatg gcgctgatgc tggaacgcgt gctcgtcggc gatgatgggg agcatgcgct
     1921 cgagtcgctc gttctggaac tcgacgacgg tcccgcagcg caggcagatg aggtgctcgt
     1981 gatgggcctg ctgtggcatc ggctcgtacc gcttgaagcc ctcgccgaaa tcgtgcgccc
     2041 ggaccagtcc gctccgcacc agcacatcca gcgtgcgata gatggtcgcg gttcctacct
     2101 tctcgccttc gtccttgagc aaccgctgaa ttgcctcgac cgaaagatgc tcgtctgaca
     2161 tgagcacggt ctgagcgacg agatcccgct ggcgggtgac cggaagatgg tggtcgcgga
     2221 gatgccggcg aaaccgctcg agcagcgccg ctccctcacc cttactgagt cgtgccatcg
     2281 gttggaggag cgagccacac cgcggggttg agggacaccg gcggttgctc gtcgtcggtg
     2341 cgcttgtgca cctcccgaag cagttcttcg atagtctcga gcggcccgct gccgacctcc
     2401 tcgacggccg cttcgaactg tccccgctcc ttgcccttga gaaccagctg ataacgcgcc
     2461 gtatagatgc ggcgtcggcc gccctcgacc cgggcgacca ccgccgtgcc ccactcgcgg
     2521 ccatcgcgcc gaatattggg aaacgcccag accgcgtcga tctcttcccg cggcaggagg
     2581 cgcgcgactt cttcggcgag ccgactccag ccgagaccca cgcccggtgc cagagcgggc
     2641 cggttcaagc cgattccgct gcccatcgct ccacgtagaa tcgaagaacg tgcatgaaat
     2701 cctgcgcgtt ggtcaccaca ccataggcct ggtgagtgcc gcggtctttg agtttgttca
     2761 ccacgaactc ggtctgatcc acgcagatgg tcatgagcgg cgccggcgga ccgtacctcg
     2821 tgtgaaacgc cggaagcatg ttgcccaccg cgatggcgtg cagcgccgtc gcgatgaaga
     2881 tcgctccggt ggcctgcacg gtatactccc gcatggcgtc ttgcgcctcg agcgtatcgg
     2941 tcagaacgcc gggcagcgga ccatcgtccc gaatcgatcc cgccagcacg aaggggatct
     3001 gatgggtcac gagcgcgtgc atgatgccgc tcttgatcag gccggattgc accgcgcact
     3061 cgattgagcc cgctgcacgc acccggttga tcgcccgcat gtgaagccca tggccgccct
     3121 gagacggcgc tccggtgctg gtcatgccca aagtggtccc gaagatcgcc gcctcgatat
     3181 cgtgcacggc caccgcgttt cccaccaaca ccgcctggac gaagccgttg gcaatgaacc
     3241 attcgaaatc ggcgcgcgcc cgggagtgta ccagggcggg ccccacaacc cagacgaggt
     3301 accccccgct ccgcttctcc tccaccagcc ggcgtgcgag ctcctcgtag ttgaccgggc
     3361 gctcgcgact cacctcggtg gacatgaatc ggaactcatt ggcgccgtga ctcccaccca
     3421 gaaatccttc ggcgtagacc acgataccct gtgagccgtc ctccgcctcg cccatcgcga
     3481 ccaggtcacc gcgcctcaac cgccgcggct cgatggtctc cagctcattg cctcgacgca
     3541 ctacgacaca atccatgcgg ggccgggcgg gcatgatcca gcgaccattt agcttgatat
     3601 aggtcggcag gttcgaggtc gagaagaagc cttcgggcaa aacgccatcg gccggaagcg
     3661 gctcgaaacg cgcctccgga gccgcgacga acggcggctt ggaaaaatcc ggaaagtggt
     3721 aggcgctggc ggcgggttga gccggggcga gatcgctcat aggcggcctc aaacctaggc
     3781 gcgcgctgag ggtgattcaa gctacaaacc gcgaccgcca ttcactaacc gtgccctgca
     3841 atggaggctc gatgccgcct cggtcgatgc tccgcgtaac cttgctcctc gcgatcctgc
     3901 tcctgagcgg agcaccgctc gccgcgcagg ggggacgtcc cgatgacggt cagaccatcc
     3961 tccgcaacat gacctacctc aagccgctgg tggggacctg gaacgcagtt gcggagtacc
     4021 atcagaagga cgggtcgctc gcctacgacg taggaaccta caagatctcg tatatcctcg
     4081 aggggacgta tctgcagtgg tacgccgagc tgcacgacaa gggcgacccc agccagcacc
     4141 actcgttcat gaatctggtg acgttcaatc cggtgaccgg ca
//
LOCUS       DASRJQ010000493         3116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3817740, whole genome shotgun sequence.
ACCESSION   DASRJQ010000493 DASRJQ010000000
VERSION     DASRJQ010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3817740"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VFB89_11310"
     CDS             complement(<1..340)
                     /locus_tag="VFB89_11310"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZM27938.1"
                     /translation="MRIDREFTLRLYRLLAAVLMFALLPAVRPMAAQTTTGSIRGYIR
                     GDNGSPIVAAELKARNLATGVSRVASTNSKGFFALSGLVPGSYAIEVRSIGFKPTGRT
                     ERVAIGQDLNL"
     gene            complement(379..1383)
                     /locus_tag="VFB89_11315"
     CDS             complement(379..1383)
                     /locus_tag="VFB89_11315"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZM27939.1"
                     /translation="MVTIRDVARAAGVSIATASRALHKGHLVAPGTREGVQAAADRLH
                     YSPHRGARSLITSRTHSIGVLLPDLYGEFFSEVIRGIDQTAQGAGYHCLLSSARHGGP
                     TLEAALRSMRGRVDGLVLMSPEFNGDLSKRALPSGFPVVLLNCPPSDTAHDSLTVANY
                     EGAHAMVRHLAGLGHRRIAIIKGAEGNFDAAERLRGYRAALKESGLAFVPELVMPGDF
                     SEAAGDAAARALLRLDQRPTAIFAANDGMAIGALYALRDAGLRVPEDIAIGGFDDIPM
                     ARYVEPALTSVHVAISVLGERAAARLFSVLEHSADRTPSRETLPTTLVVRRSCGSRGT
                     "
     gene            complement(1534..>3116)
                     /locus_tag="VFB89_11320"
     CDS             complement(1534..>3116)
                     /locus_tag="VFB89_11320"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZM27940.1"
                     /translation="LLIACANVMSLQLVRATSREREISLRLALGAGRLQLVRQLLTEG
                     MVLAGLGGIGGLILAFWCLRLLLPLAPAGLFPVYAQVGVDGRVLAFSAAITLFCGLVC
                     GLTPALRSGRGDLSDALRQGVRAASSGLRRLRKPGMQQLLVAGEVALALVLLVGAGLM
                     LKDLRVRIAVPPGFHAEDVVVARVSLPRDRYPFPERIIFAERLVERLNALPDVVGAAV
                     GTDMPLRGFSNAAYLALAVPGADPVRHFRHGVTPKFFETLGIPILAGRGFTNADLTES
                     PRVAVVSDATARRLWPGQNPLGQRFHLGDQTGPEVAVVGVAGSARFRDLTTDLGTPAG
                     GLDVYYPFPQRTDADLEIAVRTRSGAGLSAATWQEQVATIDPLLPLYLIQPLQDVVRS
                     QNAAPRFGSVVLSVFSVLALVLSAVGVYGVIAFVVGLSRREIAIRLALGADGRRVLGV
                     VMANGMALVLGGVVVGLVGARLGAKVLSSQLYGVQVTDTSTFLGVTGVVTLVAFLACW
                     LPARRAARVEPQTVLKGE"
BASE COUNT          581 a         1049 c         1053 g          433 t
ORIGIN      
        1 cgaggttcag gtcctgaccg atcgcaaccc gctcggtccg gccggtcggc ttgaagccga
       61 tgctgcgcac ttcgatggcg taagaacccg gcaccaggcc ggagagggcg aagaatccct
      121 tcgagttggt gctggccacc cgggagacgc cggtcgccag gtttctggcc ttgagctcgg
      181 ccgcgacgat cggcgagccg ttgtcgcccc ggatatagcc gcggatgctg cccgtggtgg
      241 tctgggccgc catcggccgc accgcgggca gaagggcgaa catcaggacg gccgccagca
      301 gccgatacag tcgcagggtg aattctcgat caatcctcat acagcgtcct ccgtgatggt
      361 gtgcctgctg aacgagcttc aggttcctcg agagccgcag gagcgccgca cgacgagggt
      421 cgtcggaagc gtctcacggc tgggtgtacg gtctgcagag tgttcgagca ccgaaaacag
      481 gcgggcggcg gcgcgttcgc cgagcacgct gatagccaca tggaccgagg tcagggccgg
      541 ctcgacgtac cgcgccatcg ggatgtcgtc gaagccgccg atggcgatgt cttccggcac
      601 ccggagcccg gcatcgcgga gcgcgtacag cgccccgatg gccatgccgt cattggcggc
      661 aaaaatcgcg gtcggtctct ggtcaagccg cagcaaggcc cgggcggcgg cgtcgccggc
      721 cgcctcgctg aagtcgccag gcatcacgag ctcgggcaca aacgccaggc ctgattcctt
      781 gagcgcggcg cgataccccc gcagccgctc ggccgcgtcg aagttcccct cggcaccctt
      841 gatgatcgcg atccgccggt gcccgagccc ggccagatgc cgcaccatcg cgtgagctcc
      901 ctcgtaattc gcgacggtca ggctgtcgtg tgcggtatcg gagggcgggc agttcagcag
      961 gactacgggg aatccgctgg gtagcgcccg cttcgacagg tctccattga attcgggcga
     1021 catcagcacc agcccgtcga cccgtcctcg catcgagcga agcgcagctt cgagagttgg
     1081 gccgccatgc cgggcactgg aaaggaggca gtggtagccg gcgccctggg cggtctgatc
     1141 gatgccgcga atgacctcgg aaaagaactc gccgtagaga tcggggagca gtacaccgat
     1201 cgagtgggtc cggctggtga tcaagctgcg ggcgccgcga tggggactgt agtgcaggcg
     1261 atcggccgct gcctggacgc cctcgcgggt tccgggagcc accaagtgac ccttgtgcaa
     1321 cgcacgtgaa gccgttgcga tggagacccc cgcagcccgt gcaacgtccc tgatggtcac
     1381 catgcggtgc ctcggcaaaa ccaggggatc tcagggaagc tccaaacgac ttgctgtaaa
     1441 cgtttacaac gtagtgcaaa cgtttacatt ttgccagaat cagcgaatac ttgccctctc
     1501 gcccgggccg ttaccccccc atccgaacgt ctgctactcc cctttcagca cggtctgcgg
     1561 ctccactcga gcggcccgcc gagcggggag ccaacaggcc aggaacgcga ccagggtcac
     1621 caccccggtt accccgagga aggtgctggt atcggtgacc tgtaccccat agagctggct
     1681 ggagagcacc ttggcgccca gccgcgcgcc gaccagcccg accacgaccc cgcccagcac
     1741 cagcgccatc ccgttggcca tcaccacgcc aagcaccctg cggccgtcgg cacccagcgc
     1801 gagacggatc gcaatttccc ggcgggagag acccacgacg aatgcgatca cgccgtacac
     1861 gccgaccgcc gagagcacca gcgccagcac actgaagaca ctcaggacca cggacccgaa
     1921 gcgaggggcc gcgttctggc tccgaaccac atcctggagc ggctggatga ggtagagcgg
     1981 gagcagtgga tcgatcgtcg cgacctgttc ctgccacgtg gcggcggata acccggcacc
     2041 gctgcgggtg cgcacggcga tctcgagatc cgcatcagtg cgctgcggga atgggtagta
     2101 gacatccagc ccgccggcgg gagtgcccag gtcggtggtg agatcgcgga atcgagccga
     2161 cccggccacg ccgacgacgg cgacctcggg accggtctgg tccccgagat gaaagcgctg
     2221 gccgagtggg ttctgccccg gccagagacg ccgtgccgtg gcatcgctca ccacggcaac
     2281 ccgcggtgat tcggtcaggt cggcgttggt gaagccgcgc ccggcgagaa tgggaatgcc
     2341 gagggtctcg aagaacttcg gcgttacgcc atgacggaag tgcctgaccg ggtcggcgcc
     2401 cgggacggcg agagccagat aggcggcgtt gctgaagccg cgcagaggca tgtcggtccc
     2461 gacggcggcg cccacgacgt ctggcagggc gttcagccgc tcaaccagtc gctccgcgaa
     2521 aatgatccgc tccggaaatg gataccgatc ccgcggcagc gaaaccctcg cgaccaccac
     2581 atcctcggcg tggaatccgg gaggaaccgc aatgcgcaca cggagatcct tgagcatgag
     2641 tccggccccg accaggagaa ccagcgcgag cgccacctct ccggccacga gcagctgctg
     2701 cattccaggc ttgcggagcc ggcggagtcc cgacgacgcg gcgcgaacgc cctgacgcag
     2761 cgcgtcgctg aggtccccgc gaccgctgcg gagcgccggg gtcagaccgc agaccaggcc
     2821 gcagaaaagc gtgatggcag ccgagaacgc caggacccgt ccgtcgacac ccacctgggc
     2881 gtagaccgga aacagaccgg ccggcgcgag cggcagcagg agccggagac accagaacgc
     2941 gaggatgagg ccgccgattc caccgagacc cgcgagcacc attccttcgg tgagcagctg
     3001 gcgcaccagc tgcagccggc ccgcgccgag ggcaaggcgc agggagatct cccgttcccg
     3061 ggaagtggcc cggaccagtt ggaggctcat gacgttggcg caggcgatca gcagca
//
LOCUS       DASRJQ010000494         2261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2996421, whole genome shotgun sequence.
ACCESSION   DASRJQ010000494 DASRJQ010000000
VERSION     DASRJQ010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2996421"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VFB89_11325"
     CDS             complement(<1..666)
                     /locus_tag="VFB89_11325"
                     /inference="COORDINATES: protein motif:HMM:NF024886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HZM27941.1"
                     /translation="MTATAPAPSLLTQVRDAIRVRHYSRRTEEVYVQWTRRFIRYCGM
                     RHPRQLGAAEVSSFLSSLAVEGHVSASTQNQALAALLFLYRDVLRMPVGWLNTLVRAK
                     RPARVPVVLTREEVRRVLARLKGRGSPALIAGLLYGTGMRLLEALQLRVKDIDFGANQ
                     ITVREGKGGRDRRTMLPERLKGPLLRHLAEVRTQHERDLEAGAGWVELPGALGLKYPQ
                     AGRE"
     gene            complement(765..1454)
                     /locus_tag="VFB89_11330"
     CDS             complement(765..1454)
                     /locus_tag="VFB89_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27942.1"
                     /translation="MAERVRDFLRAHQMDGVGEGLGLAKLEQLLERAQVLAGQQRIGV
                     ATTRSATRQRRQVRRTLQAKVLRYLRVVGKVAAKQNGELANQFPLPAAGESHEALLTA
                     GRATLERATAQKEVLVSLGMTQKVLDDLALALGEFEKTLEATRAGRREHVGASADLDA
                     IGTEVAEQVKLLDGVVQYRFGDDAELLAAWHSARNVAGPLPTKNGPESGAGGGGSETL
                     REAQGRPPRAA"
     gene            1985..>2261
                     /locus_tag="VFB89_11335"
     CDS             1985..>2261
                     /locus_tag="VFB89_11335"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HZM27943.1"
                     /translation="MLRHIASLALAASIAVAAHAQQPRVLVFSKTAAYRHSSIPNGIA
                     AIEKLGQENGFAVDATEDAGAFTEKNLRRYAAVVFLSTTGDVLDAAQQ"
BASE COUNT          396 a          823 c          664 g          378 t
ORIGIN      
        1 ctctcggccc gcctgcggat acttgaggcc caacgccccc ggcagctcca cccaacccgc
       61 acccgcctcg agatcgcgct cgtgctgtgt ccgcacctcg gcgagatggc gaagtaacgg
      121 gcccttgaga cgctcgggca acatcgtccg gcgatcgcga ccgcccttcc cttcccgcac
      181 ggtgatctgg ttcgccccga aatcgatgtc cttgacccgc agctgcaacg cttccagcag
      241 gcgcatcccg gtcccataca ggaggccggc aatgagcgcg ggtgatcccc gacccttgag
      301 acgcgccaac acccgccgca cctcctcacg cgtcaacacc accggcacgc gcgcgggacg
      361 cttggcccgg accaacgtgt tgagccaccc gaccggcatt ctcagtacat cgcgatagag
      421 aaacaggagc gcggccagcg cctggttctg cgtcgatgcg ctgacatgcc cctccaccgc
      481 caggctcgat agaaaactcg agacctcagc cgcacccaac tggcgtgggt ggcgcatccc
      541 acagtatcga atgaagcgac gggtccactg gacgtacact tcctcagtcc tgcgactgta
      601 atggcggact cgaatggcat cacggacttg ggtcagcaac gacggagcgg gcgcagttgc
      661 ggtcatgacc tgacgataat tgggagatgg ctggggcgcg gttcaaaaaa acgcgacgag
      721 gcgcggcagt tgatcaggct accgcgcctc cacgtgtgag ggcatcacgc cgccctcggc
      781 ggcctgccct gagcctcgcg aagggtctca ctaccgcccc cccctgcccc ggactcgggg
      841 ccgttcttgg tcggtaacgg tcccgccacg ttgcgggcgc tgtgccacgc tgccaacagc
      901 tcggcatcat caccgaaccg atactgcacg acaccatcga gcaacttcac ctgctcggca
      961 acctcggtgc caatcgcgtc gagatccgcg ctcgcgccga catgctcgcg cctccccgcc
     1021 cgcgtcgcct cgagcgtctt ctcgaactcg cccaaggcta gcgcgagatc gtcgagcacc
     1081 ttctgcgtca tgccgaggct cacgagcacc tccttctgcg cggtcgcccg ctcgagcgtc
     1141 gcccggcctg ccgtgagcaa cgcttcgtgg ctctcgccgg cggctggcag cgggaattga
     1201 ttcgccaact ccccgttctg cttcgccgcc accttgccta ccacccgcaa gtacctcagc
     1261 accttcgcct gcagcgtccg ccggacttgg cgccgctgcc tggtggccga ccgggtcgtc
     1321 gccaccccaa tccgctgctg gcccgcaagc acctgcgctc gctccagcag ctgctcgagc
     1381 ttcgcgagcc cgagcccttc acccacaccg tccatctgat gagcccgcag gaaatcacgg
     1441 actcgctcgg ccatttctag ccgcctacgc aactgcgccc gcatacaaac tccttccctt
     1501 cgttccccct ctccctcggg agagggggtc aggggatgag ggcgcacggc gtcgggctcc
     1561 gtgcgcgaat ttggaagtct cccgacgcct ccgtgagtat gcgtaaaccg ttccacgacg
     1621 gccgcgcgcg gctcgaatgt gcgcgtgtcc tttggggagg acgctttgtc gcgccggcgg
     1681 tgctcgctcg cgagaaatgg cgcgccgtat cgcgctgagc gatgtccacg cagttcgaca
     1741 cagttctcgg agtcgtgcat ggaacccgcg gaacatcgcg atggatgctc ggaacgcctt
     1801 gcgaagttcg ggaacggcac cggcagtcct cggttggcga aatcaggaag cgtactgggc
     1861 cagtccggac gcggaagtac ggattcgagc agcggaacct atccacactg cttggtgcgc
     1921 tccagattca ctgaccactt tctctttgca aagcggtagg aaaaacgggg aacaagggac
     1981 tctcatgcta cgacacattg cctcgctcgc gctcgctgcc tcgatcgctg tcgccgcaca
     2041 cgcgcagcaa ccgcgcgtgc tcgtcttctc caaaaccgcg gcctaccgcc acagctccat
     2101 ccccaacggc atcgcggcca tcgagaagct cggccaggag aatggcttcg ccgtcgacgc
     2161 cacggaggat gccggcgcgt tcacggaaaa gaacctgcgg cgctacgccg cggtcgtgtt
     2221 cctgagcacg acgggcgatg tcctcgacgc ggcccaacag g
//
LOCUS       DASRJQ010000495         4297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_600770, whole genome shotgun sequence.
ACCESSION   DASRJQ010000495 DASRJQ010000000
VERSION     DASRJQ010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600770"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..986
                     /locus_tag="VFB89_11340"
     CDS             198..986
                     /locus_tag="VFB89_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27944.1"
                     /translation="MKLLALGLETAAVLVLGAAIAGAEPLNFQGTLTIQIGDLPAMVT
                     TGGGVATLNGANGGIPSHLVSLTVAASRGNVGGTATVLVTDPGAFPGAHAIIGEFQLG
                     TGTLAPISSTLLTKNVLPVGGVLKFCQLSTACTSFLPLPLTQPTGTGGIKGLGVGGTV
                     TASNGVTQVSLFGRPWAVKATPTTFPVHFFVHGPASGTTSTAQPGGEVVLVSPSKIHS
                     SLSQDNIGVLTELTVQFVPEPGMPLLLGSGVVGLALIGRHRIRN"
     gene            1748..2032
                     /locus_tag="VFB89_11345"
     CDS             1748..2032
                     /locus_tag="VFB89_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27945.1"
                     /translation="MHRPAVSRVVLSALLVAALVGCRTVWVHAGATEQKYRDDTARCK
                     YGMTAAERDRLMADPTAQVPPHRRDWQECMQLLGWTRETEPRAHAPWDTP"
     gene            complement(2049..2195)
                     /locus_tag="VFB89_11350"
     CDS             complement(2049..2195)
                     /locus_tag="VFB89_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27946.1"
                     /translation="MSVLSAPSSRTIRTDERDEKDQAEKPAESPRRNLTAGRIHAGDV
                     EALY"
     gene            2268..2489
                     /locus_tag="VFB89_11355"
     CDS             2268..2489
                     /locus_tag="VFB89_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27947.1"
                     /translation="MGGAVRIMEDTGFGGDYIVGWGTLALINAGLAQAKNRSGLGWFL
                     ISLFFGPIATFVILLLSKVPETPPGEGAA"
     gene            complement(2793..4139)
                     /locus_tag="VFB89_11360"
     CDS             complement(2793..4139)
                     /locus_tag="VFB89_11360"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HZM27948.1"
                     /translation="MRLVLQAHLSLVVATWLTVGFPGIAHAQGPDDDGDGVPNSVDSC
                     PLVANLSRLDSDLDGYGNACDADYNNDGIVGSVDFAKLSMAYGSVEGEASWKPYADCN
                     GDDRIDDVDFFYMASVFGNPPGPSGLSCAGSGVCNLGCTGVEPPCDPRWPNLDCTYEP
                     ISFAPAYTGCAGVQPGPKILVDTTHSNNETTPDFEAGTIGKYWGFAKLLSADGYDVRD
                     SGVPLTQLLLPNVTDADILVIAAAQPNSPLPGAPSPFTSAEVVAVRDWVASGGSLLLV
                     VDHDPYNDISNLLSAFFTTVVLEGATTGSFFSPDDFVRGTVRLNVSPPIADGLTLGVN
                     KVQTFSGVAFVLTDPVPPPPDLQPPPPPQIIVPLLTFSPPAFTDDPVDISGFLQGIAF
                     TFGNGRVYLSGEAAMFTAQKDLIKFGMNATGFPPATELTNDNQRFLLNIVHWLSGL"
BASE COUNT          744 a         1382 c         1396 g          775 t
ORIGIN      
        1 gcccctccgg gatcggtgtc tgggaaatcc tgcttgcaac cgaccgatcg cgagcagacg
       61 ctgatcgctg atgaaacctt ggggagaact ctctcctatc cgcccgcggt cggccattcg
      121 gtgcggatac ggtccccgtg acctgacggg cgtccgatcc gccgtcgaag cgggcggagc
      181 gaacaggaga cgacgcgatg aagctactcg cgctcggtct tgagacggca gcagtgctgg
      241 tcctcggcgc cgcgatcgcg ggcgcggagc cactcaactt tcaggggacc ctcacgatcc
      301 aaatcggcga tctacccgcg atggtcacga ccggcggcgg cgtcgcgacg ctgaacgggg
      361 cgaatggggg aattccgagc cacctggtct cgctcaccgt cgccgcgagt cgcgggaacg
      421 tcggggggac ggccaccgtc ctcgtcacgg atccgggggc cttcccgggc gcccacgcga
      481 tcatcgggga gttccagctc gggacgggca cgctcgctcc gatctcgtcg accctgctca
      541 ccaagaatgt cctgccggtc ggcggcgtac tgaagttctg ccaactctcc accgcgtgca
      601 cttccttcct cccgctgccg ctcacccagc cgaccgggac gggcggcatc aaaggcctcg
      661 gcgtcggcgg tacggtcacg gcgagcaacg gcgtgaccca ggtctcgctc tttggccgtc
      721 cctgggccgt gaaggcgacc cccactactt ttccggtgca cttcttcgtg cacggaccgg
      781 cgtcggggac gaccagcacg gcccagccgg gcggcgaggt cgtgctcgtg tcgccgtcga
      841 agatccacag cagcctcagc caagacaaca tcggggtgct cacggaactg accgtacaat
      901 tcgtcccgga gccggggatg ccgctgctgc tcggctcggg tgtcgtgggc ctcgcgctga
      961 tcggccgtca ccggatccgc aactgagggt ccgcgaagtc cgaagtcatg tgaacccggg
     1021 tcggttgcta ggagcgcgcg gggctcgctc tccgaccccc tcgtcgccct cgcgggccgg
     1081 ctatcgctgc gagcgcagcg cgccctctcc cggaatctct cgaaggtacg ccgaggaggc
     1141 gctccaggtt ctcccggaag agcgggtttt caggcgcagc gcctccttct tgcgcggcga
     1201 cggcgcttgg agggcgctgg gtcgctattg gaaagtccgt cgagtcgcgc ccgagggtag
     1261 gggggaccct cccacaggcc ggtgcttacg gaataactgt tacccggctc ccagatacaa
     1321 cttcccggct gcgagtcgca gggtttgccg gcgaccaaac ggcggcgctg cgacggcgct
     1381 aggccgcact gccgctccgg cgggagaccc cccggcgtga ccctcccaca gaccgctttc
     1441 tacggattac cccgcctcgg cagcaagaga gtcgcggcga aaccggatga cgttcggaga
     1501 tcggctgcgg ccagggcgcg caatttcttc acctctgctg ccgttagggg aatcttcacg
     1561 cccacttcgg accttggggt ctgccttcat gcggctctgg cggcgacggg caggcatcgg
     1621 tcagcagggt gccgcaacct tgcattccgc ccaaggtttg gcggaaactc ctgccaactg
     1681 agcgggggat ctgctgacaa tgtgtgtcct ccaggcgccg cccgtcagtg acgagggagc
     1741 ggatcaaatg catcgcccag ccgtctcgcg agtcgtccta tccgcgctcc tggtcgcggc
     1801 gctagtgggc tgccgcaccg tgtgggtgca cgccggcgcc actgagcaga agtaccggga
     1861 cgacactgcg cggtgcaaat acgggatgac ggcggcggag cgcgaccggc tcatggcgga
     1921 ccccaccgcg caggttccgc cgcatcggcg cgactggcag gagtgcatgc agctcctcgg
     1981 gtggaccagg gaaacagagc cgcgagcgca tgcgccttgg gatacgccgt agtctgccct
     2041 gctttgattc agtacagggc ctcgacgtcg cctgcatgaa tgcggccggc tgtgagattg
     2101 cgcctgggcg attccgcggg cttctctgcc tgatccttct cgtcacgctc gtcggtgcga
     2161 atggtgcgac tcgacggcgc gcttagaaca ctcacgcgat tctccgcccc atctcacccc
     2221 ggtcccctgg tacattcacg ggcggccggc agctgagcgc cggtccgttg ggcggcgccg
     2281 tcaggattat ggaagacacc ggttttggcg gggactacat cgtcggctgg ggcactctcg
     2341 ccctcattaa tgctggtttg gcgcaggcga agaatcgtag cggcctcggt tggttcttga
     2401 tctcactctt ctttggaccc atcgccacct ttgttattct tcttctcagt aaggtgccag
     2461 agacgccacc gggcgagggc gccgcctaac aaggcgctgc agctgaccgg ccgccgccgg
     2521 ccggcttgcc gggctcccca gccggccggc cgcccggagc cggggtcacc cgaagcccca
     2581 attacgttca agaatgcttt cggtgagcag ctcagcgatg gggcgcgcgc cggtggcctc
     2641 gctgtgccca cgtaccaccg attcaaggct tcgggtgact gtcgaaagga tcacggcgtc
     2701 gtcgcagaag gagcggcgcc acgagatgct cgctctcgcg actccaccga tcgatactcc
     2761 acatgcacgt ccgcatcggc cggcttgttg ctctataagc ccgagagcca gtgaacaatg
     2821 ttgaggagga acctctggtt gtcattggtc aactcagtgg ccggggggaa acccgtcgcg
     2881 ttcatgccga acttgatcag gtctttctgc gctgtgaaca tggcagcctc ccctgacaga
     2941 tacacccgcc cgtttccaaa cgtgaacgca atgccctgca ggaagcccga gatgtcgacg
     3001 ggatcatcag tgaatgcggg gggcgaaaac gttaggaggg ggacaatgat ttggggcggc
     3061 ggcggcggtt gcaggtctgg aggcgggggg acggggtcgg tcagtacgaa cgctaccccg
     3121 ctgaaggtct ggaccttgtt tacgccaagc gtcagcccat cggcgatggg aggcgagacg
     3181 ttgagcctca ccgtccctcg cacgaagtcg tcgggggaaa aaaagctccc cgttgtagca
     3241 ccttcgagta ccaccgttgt gaagaatgcc gacagcaagt tgctgatgtc gttgtaagga
     3301 tcatgatcca ccaccagcag gaggctgccg cccgacgcga cccaatcgcg aaccgcaacc
     3361 acctctgcgg aggtgaaagg tgagggtgcc ccggggaggg gggaattcgg ctgggcggcc
     3421 gcgatgacta ggatgtcggc atccgtgacg tttggcagga gcaactgggt cagcgggaca
     3481 cccgagtctc gaacgtcata gccgtcggcg gagagcagct tcgcaaaccc ccagtacttt
     3541 ccaatcgttc ccgcctcgaa gtccggcgtg gtttcattgt tcgagtgcgt ggtatcgacc
     3601 aggatcttgg gtcccggttg aacgccggcg catccggtgt atgcgggagc gaacgagatc
     3661 ggctcgtacg tgcaatcaag attgggccaa cgagggtcac acgggggctc cacacccgtg
     3721 catccgaggt tgcagacccc gcttcccgcg cacgacaagc cagaaggtcc cggcggattt
     3781 ccaaacactg atgccatgta gaagaaatcg acgtcatcga ttcggtcgtc gccgttgcag
     3841 tccgcgtacg gcttccagct tgcctcgcct tcgacggatc cgtacgccat cgagagcttc
     3901 gcgaagtcta ccgatccgac gatgccgtcg ttgttgtaat cggcgtcgca cgcgttccca
     3961 taaccgtcga gatcggaatc cagccggctc aggttggcga ccaacgggca gctgtcgacg
     4021 gagttcggaa ccccgtcgcc atcgtcatcc ggcccttgcg catgtgcaat tcctggaaac
     4081 ccaactgtta gccacgtcgc taccacgagt gagagatgag cctggagcac caatctcatt
     4141 gtcttctcct tcctcgagcg gcggaatcag cgccggggtc gcagctcggt tgcggcacta
     4201 taccgagagt cccgcgagat ggggtcgcgt gaggaagcaa atctctacct agagcaagcg
     4261 caggcattcg ggtatccgcg tatattgctt caggatg
//
LOCUS       DASRJQ010000496         3172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_853685, whole genome shotgun sequence.
ACCESSION   DASRJQ010000496 DASRJQ010000000
VERSION     DASRJQ010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853685"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            286..1632
                     /locus_tag="VFB89_11365"
     CDS             286..1632
                     /locus_tag="VFB89_11365"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HZM27949.1"
                     /translation="MSKTGDPAAITGMVHTTVALAGIQGEVHSYSPSGIPLWVQSMEP
                     IGLAAGMHLTWEPTATGARFVLFADQGAPLPPTSARRPTQFLRIQWLPGIRNTRDPGI
                     IDPSTRWHVAWNDLFGSDIDGNLVPLCPLGTPGIVADVANICVGGPGLCDMNGDAQAD
                     VRDLVLMAHCLVSPRACPWDLTNLDCDGDTDVDFDDLYCCARTVLGVPPCPTCGDSVR
                     VESGIALRFSTPQRGTGVIEVPMRIDGAGLIGAARLTLAMPPGLAGTATLDLQDSHWL
                     RVTRANGDRLEVGLIGIPSNGLDRQPLPLYLEATLRITLPGGSDASGQIALEGVDLSG
                     PDGVKLKADLGSPVVRLGPGLGISLSQARPNPFGASTAFRLTLDAPADAADLAVYDLS
                     GRRITTIHKGPLPIGSHAFTWDGRMEAGGRARAGVFFVRASVAGHTSIQKLVMLRD"
     gene            complement(1662..2015)
                     /locus_tag="VFB89_11370"
     CDS             complement(1662..2015)
                     /locus_tag="VFB89_11370"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZM27950.1"
                     /translation="MGVHRRERSGIVVLDISGEFYGDKETDHLEAAIQNELAAGSLHL
                     ILNLVACSWMNSTALGVMMRAKMAYDARGGNIRICGAERRMKSLLVVLRLYDWFDHHE
                     TEADAVEAFARPAEV"
     gene            2014..2496
                     /locus_tag="VFB89_11375"
     CDS             2014..2496
                     /locus_tag="VFB89_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27951.1"
                     /translation="MTHLLTSADGATPLITGLLGRSTTVSQRGWEFSPPGRANEKELE
                     VSSCGSLPWEAIQGLSLGVLLAVAAGHPDGRFSEPATLGATVRPEVREEDAAASVRAE
                     RHGLGGPGAAAERHLRPVPTAVVRPFASPNYGTSRSDGARLQLWVAKTFRTSGAAKPR
                     "
     gene            complement(2465..>3172)
                     /locus_tag="VFB89_11380"
     CDS             complement(2465..>3172)
                     /locus_tag="VFB89_11380"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HZM27952.1"
                     /translation="GVVLGWGRTFGLLHRQALVTATLPYAFADISGNVYEQQQSVTRS
                     GLADFRARVALNLYGSPAQTPQEFAGRRRPPLLVGTSLTVTAPTGQYFGDKLINIGTN
                     RWAFKPEVGVSVPWKTFDFDVYAGAWLFTDNADFFPGGQSRHQDAVSTVQGHVSYTLR
                     PSLWLAADGTWYGGGASSVGGGPKSGRLDNTRLGGTLSLPLTASQSAKFAYSRGALVR
                     AGSDFSTIIVAWQLRWF"
BASE COUNT          520 a         1061 c         1033 g          558 t
ORIGIN      
        1 ctggtgttcg acaattcgtg ctgcgctggc cgcgtgtgtg tcgcgttccc gacccagttc
       61 caggcctcat tgatcattcc ccccgtctac ccgggatggc agaacctgac catggtgggc
      121 tacgagacct gttgccgcga agagccggtg cagaccgaac cggtcggcta tcggaacttc
      181 gggttctacg tcatgtccgc cgagagctgc ggagtcgagc cgccgccgat cacgtgcctg
      241 tggcccacgt ggctgcacga tcagaccgcc gagtgcgacg cacacgtgag caagaccggc
      301 gatccggccg cgatcacggg catggtgcac acgaccgtgg cgctggccgg gatccagggc
      361 gaagtccact cgtactcgcc gagcggaatt ccgctgtggg tccaatcgat ggagccgatc
      421 gggctcgccg ccggcatgca tctgacctgg gagccgactg cgaccggcgc gaggttcgtt
      481 ctgttcgcgg accagggcgc gcctcttccg cccacctcag cccgtcgccc cacacaattc
      541 ctgaggattc aatggctgcc cgggatccgc aacacgcgcg accccgggat cattgatcct
      601 tccacgcgct ggcacgttgc gtggaacgac ctgttcggat cggacatcga cggcaacctg
      661 gttccgctgt gcccgctcgg cacgcctggc atcgttgccg atgtcgccaa catctgtgtc
      721 ggcgggccgg gtttgtgcga catgaacggc gatgcgcagg ccgacgtgcg cgacctggtg
      781 ctgatggcgc attgcctggt cagcccccgc gcgtgcccgt gggacctcac gaatctcgat
      841 tgtgacggcg acaccgacgt cgatttcgac gatctgtact gctgcgcgcg caccgtgctc
      901 ggcgttccgc cgtgtccgac gtgcggagac tcggttcgtg tggagtcagg gatcgcactt
      961 cgcttcagca caccccagcg cggcacgggc gtgatcgaag tcccgatgcg gatcgacggg
     1021 gccggcctga tcggtgccgc aaggctgacg cttgcgatgc cgccgggact cgccggcacg
     1081 gcaacactcg acctgcagga ctcccactgg ctgcgagtga cccgcgctaa tggcgatcgt
     1141 ctcgaagtcg gactgatcgg gattccgagc aacggcctcg accggcagcc gctgccgctg
     1201 tacctggaag ccacgctgcg gatcaccctg ccgggcggct ccgacgcgag cggacagatc
     1261 gcgctcgagg gcgtggatct cagcgggccc gacggagtga agctgaaggc cgacctcggc
     1321 tccccggtcg tgcggctcgg tccgggcctc ggcatctcgc tgtcccaggc gcgcccgaat
     1381 cctttcggcg ccagcaccgc gttccggctc acgctcgacg cccccgcgga cgccgcggat
     1441 ctcgcggtct acgacctgtc gggtcgccgc atcaccacga tccacaaggg gccgctgccg
     1501 atcggcagcc acgccttcac gtgggatggt cggatggaag ccggcggacg cgccagggcg
     1561 ggcgtgttct tcgttcgcgc ctcggtcgcg gggcacacca gcatccagaa gctggtgatg
     1621 ctgcgcgatt gatggcggga tgagcggcga cgacgactcg atcagacttc ggccggcctc
     1681 gcgaaagctt ccacggcatc ggcttcggtt tcgtggtgat cgaaccagtc atagagtcgc
     1741 agcacgacca gaaggctctt catcctccgc tccgcgccac aaatcctgat gtttccgcct
     1801 ctggcgtcgt aggccatctt ggccctcatc atcaccccca gcgcggtcga attcatccag
     1861 ctgcacgcga ctaggttcag gatcaggtgc aggcttcctg ccgccagttc gttctggatc
     1921 gcggcttcga ggtgatcggt ttctttgtcc ccgtagaatt caccgctgat gtcgagaacg
     1981 acgatccccg aacgctctct cctgtggacc cccatgacac acctcctcac gagcgccgat
     2041 ggagccacac cgctcatcac cgggcttctt ggtcgtagta caacggtgag ccagcggggt
     2101 tgggagtttt cacccccggg gcgtgccaac gagaaggagc tagaggtctc ctcctgcggt
     2161 agcttgccgt gggaagccat tcagggactc tcgcttggtg tgttgctcgc cgttgccgcc
     2221 gggcatcctg atgggcgatt cagcgaaccc gcgacacttg gagccacagt ccgccccgag
     2281 gtgcgcgagg aggacgcagc tgcgtccgtt cgcgccgaac gacatggact tggtggaccc
     2341 ggagcagcgg ccgagcgaca ccttcgaccc gtaccgaccg cggtggtgcg acccttcgcg
     2401 tcacccaact acggcaccag ccgctcggac ggagcgcgcc ttcagttatg ggtggcgaaa
     2461 acgttcagaa ccagcggagc tgccaagcca cgatgatggt gctgaagtcg gagccggcgc
     2521 gcaccagggc gccgcggctg taggcgaact tcgccgactg gctcgcggtg agcggcaggg
     2581 acaacgtccc tccgaggcgg gtgttgtcga gtctcccgct cttcggacca ccaccgaccg
     2641 aggaagcgcc tccgccatac caggtaccgt ccgccgcaag ccacagcgaa ggtcgtagcg
     2701 tgtagctcac atgcccctgc acggtcgaaa ccgcgtcctg gtgacgggat tgtcccccgg
     2761 gaaagaagtc cgcgttgtcg gtgaagagcc aggctccggc gtacacgtcg aaatcgaacg
     2821 tcttccacgg gaccgatacg ccgacctcgg gcttgaatgc ccagcggttg gtcccgatgt
     2881 tgatcagctt gtcgccaaag tattggccgg tcggggccgt tacggtgagg ctcgtgccga
     2941 caaggagtgg cgggcgcctc cttcctgcga actcctgcgg cgtctgtgcg gggctcccat
     3001 agagattgag ggcaacccgg gctctgaagt ccgcgagccc ggatcgtgtg acgctctgct
     3061 gttgctcgta gacattgccg ctgatatccg caaaggcgta gggcagagtc gcggtcacga
     3121 gcgcctgccg gtgaaggagc ccaaaggtac ggccccagcc gagcacgacg cc
//
LOCUS       DASRJQ010000497         2163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_27263, whole genome shotgun sequence.
ACCESSION   DASRJQ010000497 DASRJQ010000000
VERSION     DASRJQ010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27263"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..1317)
                     /locus_tag="VFB89_11385"
     CDS             complement(292..1317)
                     /locus_tag="VFB89_11385"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HZM27953.1"
                     /translation="MSTIVAGRPVPLSRVWSGSKIIAEDLTGEDLSDVLELHSDASAW
                     WVLPRDSDYGAIELRHVARALDLDELAIKDLLADDKRAKFEVIGQARLVITNAVSVHE
                     DNAEILVHPVSMIATDRAVICLMDPTPDFQPAQLLVEKQDVIAQGGIEAAVQTVIAAA
                     IRTYEEALRWLEDSSDDLANVLFEERPLNKPEQLHAFKLRSALSQLRRLTEPMRTVMR
                     DIVDSPPQGRKGKPGRDPAIGRHWVMLAEQHDRVANAADALREALSSIFDTSLALADV
                     RMSQIMKKLSGWAAIIAVPTLVTSFVGMNVGFPLHDSSAGFWVYFLLMITAGVVLYFI
                     FRARDWL"
     gene            complement(1404..>2163)
                     /locus_tag="VFB89_11390"
     CDS             complement(1404..>2163)
                     /locus_tag="VFB89_11390"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZM27954.1"
                     /translation="GTAASPAEASTPSPAAPSATPGRTESTKPGEPVRFAGPAGELQG
                     AWAEAGDPQGALLVVHENRGLTPHFFDLVGRFAGASYSALCVDLLSSQGGTGSLQDPA
                     AAPTTLAKTAPEQLVADLQAGIDELQKRVPDAKVGVVGFCFGGGMTWNLLQAGEQRLA
                     AAVPFYGPAPENPDFSKAKAAVYAIYGEQDERVNASRERAEAALKAAALTYEIRTFAG
                     AGHAFFNDTGPRYNAEAAQQAWQVMLDWFNQHLR"
BASE COUNT          370 a          727 c          666 g          400 t
ORIGIN      
        1 gacggcagca ggtgcaaggc gtagcgctcg ccgttcacga ccacagtgtg gccggcgttg
       61 ttgccgcctg aataacggac gcagtagtcg acgcgatcac cgagttgatc cgtcgccttt
      121 cccttcccct cgtcgcccca ctgggctccc atgaccacaa tcgccggcat ctgatcgccc
      181 tcccttctag ctccctactg cgtagagaaa taccgaagcc cctgggcgca ttcgccacag
      241 ggactcttgc gacgtaaccc taccgaaacc tcctttgagc tgtcgcctga gttacagcca
      301 atccctggct cggaagatga agtacaacac gacgcccgct gtgatcatga gcaggaaata
      361 gacccagaag ccggcgctac tatcgtgcaa cgggaagccg acgttcatcc cgacgaagct
      421 ggtgaccagg gtgggaacgg cgatgatcgc ggcccatccg ctgagcttct tcatgatctg
      481 gctcatgcgc acatcggcaa gcgcgagact ggtgtcgaaa atcgacgaca gtgcctcacg
      541 cagtgcatcg gcggcgttgg cgacgcggtc gtgctgctcc gccagcatca cccagtgacg
      601 tccaatggca ggatctcgtc ctggcttgcc ctttcggccc tggggcgggc tatcgacgat
      661 gtcgcgcatc accgtccgca tcggctcggt gagccggcgc agctgggaaa gagcggagcg
      721 gagcttgaaa gcgtgcagct gttcaggctt gttgagcggt cgctcctcga acagcacgtt
      781 tgcgaggtcg tcgctggaat cctcgagcca ccgaagcgcc tcctcatagg tgcgaattgc
      841 cgccgcgatc accgtctgga cggcagcctc gatgccgccc tgggcaatca cgtcctgctt
      901 ctctacgagc agttgggccg gctggaagtc cggcgtcgga tccatcaggc agatcacggc
      961 acgatcggtc gcgatcatcg aaaccgggtg caccaggatc tctgcgttgt cctcgtgaac
     1021 cgagaccgcg ttggtgatga cgagccgcgc ctggccgatc acctcgaact tggcgcgctt
     1081 gtcgtcggcc agcaggtcct tgatagccag ctcgtcgagg tcgagggcac gcgcgacgtg
     1141 gcggagctct atcgccccgt agtcggaatc tcgcggcaag acccaccatg ccgatgcgtc
     1201 ggaatgcagc tccaagacat cgctcagatc ttcaccggtc aggtcctcgg cgatgatctt
     1261 gctgcccgac cacacccgac tcagcggtac aggtcggcca gcgacgatgg tggacaccct
     1321 ctgaagccta cgcgccactc ggctacccgc cctcagacgc tgctccggct accacgctgt
     1381 ccagtcggtt cgcctctcgc ccatcacctc aggtgctggt tgaaccagtc cagcatcacc
     1441 tgccacgcct gctgggcggc ctccgcgttg taacgagggc cggtgtcatt gaagaaggcg
     1501 tgaccggctc cggcgaaggt gcgaatctcg taggtcagtg cggccgcctt gagtgccgcc
     1561 tcggcgcgct ctcggctcgc gttcacccgt tcgtcctgtt ccccgtagat cgcgtaaacg
     1621 gctgccttgg ctttgctgaa gtcggggttt tcgggcgctg gtccgtagaa cgggacggcc
     1681 gctgccaggc gctgttctcc agcttggagc aggttccagg tcataccgcc accgaagcag
     1741 aacccgacga ctccgacctt cgcgtccggc acacgcttct ggagttcgtc gattccggcc
     1801 tggaggtctg caaccagctg ctcgggcgcg gtcttcgcga gagtggtcgg tgctgccgct
     1861 ggatcctgca gcgaccccgt gcccccttgc gatgagagca ggtcgacgca cagcgccgag
     1921 tacgaggcgc cggcgaaccg cccaacaagg tcaaagaaat gcggagtcag accgcgattc
     1981 tcgtggacaa ccagcagcgc gccctgtggg tcccccgcct ccgcccaggc cccttgcaac
     2041 tctccggccg ggccggcaaa gcgcaccggt tctcccggct tagttgactc ggttcttcct
     2101 ggggtggctg aaggggccgc tggcgagggc gtcgaggctt ccgcaggcga cgccgctgtc
     2161 ccc
//
LOCUS       DASRJQ010000498         5712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_4145964, whole genome shotgun sequence.
ACCESSION   DASRJQ010000498 DASRJQ010000000
VERSION     DASRJQ010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5712
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4145964"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1844
                     /gene="uvrA"
                     /locus_tag="VFB89_11395"
     CDS             <1..1844
                     /gene="uvrA"
                     /locus_tag="VFB89_11395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZM27955.1"
                     /translation="LERARQLDVDPGVSWSKLKAAQKRELLYGRKGRYVGIFPFLKGL
                     EEKRYKQYIRVFLRQYQLARTCEACNGTRLNGDALSVRIGGDTIAAVAARSVDDIHTW
                     VRSLTLSSFEREVGQLILDQLDARLGFLCDVGLGYLTLDRQTRTLSGGEAQRIALANA
                     LGSKLVDALYVLDEPSIGLHPRDTDRLLALLRRLRDNGNTVVVVEHDLSAIQQADFML
                     ELGPGSGERGGRVVHAGPVGTAGASLTGQYLTGAKRIAVPSMRRHAGPQWLRIRGAEL
                     HNLKGVDADIPLGTLTAVTGVSGSGKSTLVYDVLYRNLESRLHGGHSAKSHLGEEVGL
                     VRSLTGWEHLEDVLLVDQSPIGRSPRSNPVTYIKAFDEIRDLFAEQPLARQRRYSAAT
                     FSFNLPGGRCEACEGAGHVQVEMVFLADVFVPCEVCGGSRFKPEVLDVKIHGQSIRDV
                     LQWTVDEAVARFRHQVKLGAALWHLQQVGLGYLRLGQPATTLSGGEAQRLKIARELSS
                     SAKRDGRKLYLLDEPTTGLHLDDVRVLIQVLDRLVDAGHTVVVIEHHPDVVKRADWIV
                     DLGPEAGDAGGRLVAQGTPEHVAAVAASHTGRYLRPLLLPTAPALAG"
     gene            1841..2728
                     /locus_tag="VFB89_11400"
     CDS             1841..2728
                     /locus_tag="VFB89_11400"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZM27956.1"
                     /translation="MTRRQGIVALIFIAVVWGCCFTLIKQTLAAVSPLLFLAIRFLLA
                     SVFVLPWLRGITRRELAGGLVLGVLFWAGFVFQTVGLAYTTPSRNAFITGLSTPLVPV
                     AYFLVYRRVPSFAVGVAVIVTGVGMYLLTRPGGTSGGINIGDLLTLGCAVLFAFQIVA
                     AGHFAPTARPDRLLALELTATGLLSAMAAPFLETPSLALDAGTVVAIVFLSVTAAVTF
                     YLQLGAQRVVSPALTAIIFTLEALAAAVTSWIVLNETLSVGQWTGGFLILLGAVLPFV
                     GVGRWRAPAPYPAKMEPES"
     gene            complement(2736..4181)
                     /locus_tag="VFB89_11405"
     CDS             complement(2736..4181)
                     /locus_tag="VFB89_11405"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HZM27957.1"
                     /translation="MTPDEFRAHGHRLVDWMADYLRDVGRQRVVPDVQPGDIRRAVSN
                     APPSDGESFDTIMKDFERVILPGITHWNHPGWFAYFAGNNSPPSILAEMLTATLGAQC
                     MSWQTSPAATELEQVTMSWLAQMLALPSGFTGVIQDTASTATLVALLSARERAAKFAG
                     ERLGTNAASGLRCYASSEAHSSVVKGVKLAGYGAEALRSIPVDRSYALRPEMLEEAIV
                     RDVSAGHVPACVVATVGTTSSTAIDPLPAIAEVCRRHGAWLHVDAAWAGSAAILPELR
                     WILDGVEHADSLVFNPHKWLLVNLHCTAYFVRDVDTLLRTFSVTPEYLRTARDSEVVN
                     YRDWGIQLGRRFRALKLWFVIRSYGVEGLQAMIRRHIELAKEFASWVESHPDFELLAP
                     SPLGLVCFRYRPGTGSLDEFRLAQLNRELLARVNASGRVFLTHTDLAGRYAIRLAVGQ
                     RCTEAPHVQEAWRLLREAASAVDARTATQQP"
     gene            4306..4959
                     /locus_tag="VFB89_11410"
     CDS             4306..4959
                     /locus_tag="VFB89_11410"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HZM27958.1"
                     /translation="MAQPLPTDLQGLCFDPVEAVAYLTRADPELAAVIRQAGPFTMRP
                     TRTQSLFAALLEAIVYQQLHGRAAETILGRVHALFGPRRHPRAEDLLRIPESTLRSAG
                     LSRNKAAALQDLARRVIDGTVPSLRSARGLSDEELMERLTAVRGVGRWTVEMLLIFRL
                     GRPDVLPVTDLGIRKGFRKAFRMRRLPAVVTMVRRAERWRPYRSVASWYLWRAAERD"
     gene            complement(4956..>5712)
                     /locus_tag="VFB89_11415"
     CDS             complement(4956..>5712)
                     /locus_tag="VFB89_11415"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HZM27959.1"
                     /translation="AVLLAGIIIGTSINLPRLLAVLQERGGLAEDAARGAVIAAAAAC
                     CAPFVIGMVRSSRRLGDALADLALPSRGAGKMDPADAPRRAFIVALQIAIALMVGIPL
                     VAVTQPFLPAFRGVLIVLLALAILGLLFWRTATNLQGHMRAGAEIVAEALGPKHRGTE
                     AATLEQVQHLLPGLGTLVPVTVAPGSVADGITLAQLNLRGRTGATAVALSRGSEATVL
                     PTADTLLREGDMLALSGSHDAIAEARELLTRVG"
BASE COUNT          959 a         1874 c         1868 g         1011 t
ORIGIN      
        1 tgctcgagcg agcgcggcag ctcgacgttg atcccggcgt ctcatggtcg aagctcaagg
       61 cagcccagaa gcgcgagctt ctctacggga gaaagggccg ctacgtcgga attttcccct
      121 tcctcaaggg actcgaggaa aagcgctaca agcagtacat ccgggtattc ctccgccagt
      181 atcagctcgc gcgcacctgc gaggcgtgca acgggacccg gctcaatggc gatgccctga
      241 gcgttcggat cggaggcgac accatcgcgg cggtagcggc ccgctcggtg gacgacatcc
      301 acacctgggt ccgcagcctc accctgtcgt cgttcgagcg ggaggtcggt cagctcatcc
      361 tcgaccagct ggatgcccgg ctcggctttc tctgcgacgt ggggttgggg tacctgacgc
      421 tcgatcggca gacccgaaca ctctcggggg gcgaggcgca acggatcgcc ctggcgaacg
      481 cgctgggctc gaagctggtc gatgccctgt atgtgctgga cgagccgtcg atcggcttgc
      541 acccacgtga taccgaccgg ctcctcgccc tgctccgccg tctgcgcgac aacggaaaca
      601 cggtcgtggt agtggaacac gatctctcgg ccatccaaca ggccgatttc atgctggagc
      661 tgggtcccgg ctcgggtgag cgcggcggca gggtggttca cgcgggaccg gtgggcactg
      721 ccggcgcatc cttgaccggc caatatctca cgggcgcgaa gcggatcgcg gtgccaagca
      781 tgcgacgcca tgcgggaccg cagtggctcc gcattcgtgg ggccgagctg cacaacctga
      841 agggggtaga tgcggacatc ccgctcggca cgctcacggc ggtgactggc gtatccggat
      901 cgggcaagag cacgctggtg tacgacgtgc tataccgcaa tctcgagtcg cgactccacg
      961 gaggccactc ggcgaagtct catcttggtg aggaggtcgg gctggttcgc agtcttacgg
     1021 gatgggagca cctggaagac gtcttgctgg tcgatcagtc gcccattggg cgctcgccgc
     1081 gatcgaaccc cgtcacgtac atcaaagcct tcgatgagat tcgagacctg ttcgcggagc
     1141 aacctctagc ccgccagcgc cgctacagcg ccgccacgtt ctcgttcaat ctgcctggcg
     1201 gccgctgtga ggcctgcgag ggtgccggcc acgtccaggt cgagatggtg tttcttgcgg
     1261 atgtgttcgt gccgtgcgaa gtgtgtggcg gcagccgatt caagcccgaa gtgctcgacg
     1321 tcaagatcca cggccaatcg attcgggacg tgctccagtg gacggtagat gaagccgtgg
     1381 cccgctttcg ccatcaggtc aagctcggtg ccgcgctctg gcacttacag caggtcgggt
     1441 tgggctacct ccgcctgggt cagccggcca ccactttgtc aggcggggag gcccagcggc
     1501 tcaagatcgc acgtgagctg tccagctctg ccaagcgcga cggacgcaag ctctacctgc
     1561 tcgacgaacc caccaccgga cttcatctcg acgatgttcg cgtgctcatc caggtgctcg
     1621 atcgcctggt ggatgcgggt catacggtgg tcgtcatcga gcaccacccc gatgtcgtca
     1681 aacgggccga ttggatcgtg gacctcggcc ccgaagccgg cgacgccggc ggtcggctgg
     1741 tggcgcaggg aacgcccgag catgtcgccg cggttgcggc ctcgcacacc ggccgctact
     1801 tgcggcccct gctcctcccg acggcgccgg ctctagccgg atgacgcgtc ggcagggcat
     1861 cgttgcgctc atattcattg ctgtcgtctg gggctgttgc tttaccctca tcaagcagac
     1921 cctggcggcc gtttcgcccc tgctcttcct cgcgatccgc ttcctgctcg cctccgtctt
     1981 cgtgctaccc tggctgcgcg gcatcacgcg gcgggagctg gcgggcgggc tggtgctggg
     2041 cgttctgttc tgggcaggat tcgtgtttca aacagtcggc ttggcatata ccacgccaag
     2101 tcggaatgcg ttcatcaccg gactgagcac cccgttggtc cccgtcgcat acttcctggt
     2161 gtaccggcga gtcccttcgt tcgcggttgg cgtcgccgtc atcgtgaccg gcgtgggcat
     2221 gtacctgctc acccggccag gcggaaccag cggtgggatc aacatcggag acctgctgac
     2281 tctcggctgc gccgtgctgt tcgcctttca gatcgtagcc gcgggccatt tcgcgcccac
     2341 cgcccgaccg gataggctgc ttgcgctgga gctcacggca accgggctct tgtcagccat
     2401 ggctgcgccg ttcctcgaga cgcccagtct cgccctcgac gcgggaaccg tggtggcgat
     2461 cgtgtttctc agcgtcaccg ccgccgtgac cttctatctc cagctgggtg cccaaagggt
     2521 cgtgagtccg gccctgaccg cgatcatctt caccctcgag gcgctcgcgg ctgctgtgac
     2581 cagctggatc gtgctcaacg aaacgctgtc tgttgggcag tggaccgggg gcttcctgat
     2641 tctcctgggt gcggtgttgc cctttgtcgg agtcggccgg tggcgcgccc cggcgcctta
     2701 tccggccaag atggagccgg agtcgtgagg gttggctacg gctgctgcgt tgccgttcgc
     2761 gcgtcgactg cggaggccgc ttcacgcaac agacgccacg cctcctggac gtgtggggct
     2821 tcggtacagc gctggccgac ggcgagccga atggcgtatc gacccgcgag atcggtgtga
     2881 gtcaggaaga cccgcccgct cgcgttaacc cgcgcgagaa gctcgcggtt gagctgcgca
     2941 aggcgaaact cgtcgagcga gccagtcccg gggcgatagc ggaagcagac taggccgagg
     3001 ggactcggcg ccagcaattc gaagtccggg tgcgactcga cccacgacgc gaactccttt
     3061 gccagttcga tgtgccgccg gatcatggcc tggagcccct cgaccccata gctccggatc
     3121 acaaaccaca gcttgagcgc gcggaagcgg cgccccagct ggatccccca gtctcggtag
     3181 ttgaccacct cgctgtcacg tgccgtgcgc aagtactccg gagtcacact gaaggtccgg
     3241 agcagcgtat cgacgtcgcg gacgaagtac gcagtgcaat gaaggttgac cagcagccac
     3301 ttgtgcggat tgaacacgag gctatcggcg tgctctactc cgtccagaat ccatcgcagc
     3361 tcaggaagaa tcgccgcgga cccggcccac gcggcatcta catgcaacca cgccccgtgg
     3421 cgccgacaga cctccgcgat ggcggggagg gggtcgatgg cggtagacga tgtcgtgccg
     3481 accgttgcca ccacgcatgc cggaacgtga cccgctgaga cgtctcggac gatcgcttcc
     3541 tccagcattt cgggccgcag agcgtaggat cgatcgaccg gaatggagcg cagcgcctcg
     3601 gctccatagc ccgcgagctt gacccctttc acgacagacg aatgcgcttc ggacgacgca
     3661 taacagcgga gcccggatgc ggcattagtg cccaggcgct cgcccgcgaa tttggcggcc
     3721 cgctcgcgcg ccgaaagaag cgccacgagg gtcgccgtag acgccgtatc ctggattacc
     3781 ccagtgaatc cactcggcaa ggcgagcatc tgcgccagcc agctcatggt cacctgctcc
     3841 agctcggttg ccgccggcga cgtctgccac gacatgcact gagcgccgag ggttgcggtc
     3901 agcatctcgg cgagaatcga gggcgggctg ttgttgccgg cgaagtaggc gaaccagccg
     3961 ggatggttcc agtgggtaat tcccggcaag atgacacgct cgaagtcctt catgatggtg
     4021 tcgaacgatt cgccgtccga aggtggagcg ttcgacacgg cacggcggat gtcgcccggt
     4081 tgtacgtccg ggacaacacg ctgccggcca acgtcccgca ggtaatcagc catccagtcg
     4141 accagtcgat ggccgtgagc gcggaattcg tcgggggtca tggggtggga aggtaagagg
     4201 ggaaaagcgt gaacggtaaa cggtgaacgg tgaacggggg ttcggaccat cagcggagcc
     4261 cctgtttact gttcactgtt caccgttcac gctttttcta gaactatggc ccagccactt
     4321 cccaccgact tgcaaggcct ctgcttcgat ccggtcgaag cggtcgctta cctcactcgc
     4381 gcagacccgg agcttgccgc ggtcattcgg caagctggcc ctttcaccat gcggcccact
     4441 cgaacccaga gtctcttcgc ggccctgctg gaggcgatcg tctatcagca gctgcacggc
     4501 cgcgccgccg agaccattct cgggcgggtg cacgctttat tcggcccacg ccgccatccg
     4561 cgggccgagg acctgttgcg catcccggag tcgacccttc gatcggcggg gctctctcgc
     4621 aacaaggcgg cagcgctcca ggatctggca cgccgagtga tcgatggaac cgtaccatcg
     4681 ctccggagcg cccgaggctt gagcgacgag gagctgatgg agcgcctcac ggcggttcgc
     4741 ggcgttggcc gctggacagt ggaaatgctg ttgatcttca ggctgggtcg gccggatgtc
     4801 ctgccggtga ccgacctcgg catacgcaaa gggttccgca aggcgttccg catgcgccgc
     4861 ctgcccgcgg tcgtcactat ggtccgccga gccgagcgat ggcggcccta tcgcagcgtg
     4921 gcgagctggt atctgtggcg tgccgcggag cgcgactaac ccacccgggt cagcagctct
     4981 ctcgcctctg cgatggcgtc gtggcttccg gagagcgcca gcatgtcgcc ttcgcgaagc
     5041 agagtatcgg cggtgggtag tacagtggcc tcgctcccgc gcgagagcgc gaccgcggtg
     5101 gcgccggtac gcccacgcag gttgagctgc gccagcgtga tcccatccgc cacactgccc
     5161 ggggccactg tcaccggaac cagcgtcccg agaccgggaa gaagatgctg cacctgctcc
     5221 agcgtcgctg cctcggttcc gcggtgcttc ggtcccagtg cttcagccac gatctcggcg
     5281 cctgcccgca tgtggccctg caggttcgta gcggtcctcc agaacaagag accgagaatg
     5341 gccaacgcca gcagcacgat cagcacaccc cggaaagcgg ggaggaacgg ctgggtcacc
     5401 gcaaccagcg gtatcccaac catcagggca attgcgatct gaagcgctac gatgaacgcc
     5461 cgccgaggcg cgtccgcggg atccatcttg ccggcccccc gactcggcag cgccagatca
     5521 gccagagcat cgccgaggcg gcgagacgat cgcaccatcc cgatgacaaa tggggcacaa
     5581 cacgccgcag ccgccgcgat aacggctcct cgggcggcgt cttcggccag tccgccgcgt
     5641 tcctgcaaaa cggcgagcag tcgggggagg ttgatcgagg ttccgatgat gattccggcg
     5701 agcaggacgg ca
//
LOCUS       DASRJQ010000499        13052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1845095, whole genome shotgun sequence.
ACCESSION   DASRJQ010000499 DASRJQ010000000
VERSION     DASRJQ010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 13052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13052
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1845095"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /gene="ruvB"
                     /locus_tag="VFB89_11420"
     CDS             complement(<1..955)
                     /gene="ruvB"
                     /locus_tag="VFB89_11420"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HZM27960.1"
                     /translation="MAQIQITTPAALPDEPAADAALRPSRLDEFVGQAQVKASLQIAV
                     EAARARKDTLDHILFFGPPGLGKTTLAMLMAREMGVQLRTTSGPVLEKPGDLVGLLTG
                     LQARDMLFIDEIHRLRPALEEYLYSAMEDFRVDVRIADGPHAQTIPMSLERFTLIGAT
                     TRFGQLTPPMRARFGIVERLGFYPTEDLRTIVTRSAQILKVPIDEEGTLEIARRSRGT
                     PRVANRLLRRVRDYAQVRAGGVITGPVSNDALSRLNVDEFGLDDMDARILLTIIEKFG
                     GGPVGLNTVAVAVGEDAGTLEEVYEPFLIQQGFMERTPRGRC"
     gene            complement(1247..1840)
                     /gene="ruvA"
                     /locus_tag="VFB89_11425"
     CDS             complement(1247..1840)
                     /gene="ruvA"
                     /locus_tag="VFB89_11425"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HZM27961.1"
                     /translation="MIASIAGVLADREGETVVVQTDGGVGYAVTVPLGVFERLPPRGA
                     RVNLYTELVVREDGWALFGFDRPTDRVIFQRLLGASGFGPKLALAVLSALGPDRTVRS
                     IRDRDIAALSTVSGIGRKKAERLVLELRDRFDDVSVGPAERPSTGSEHAVRALLALGY
                     APAAAEEAVRSVVANGATGDTAAVVRRALAALTGGSK"
     gene            complement(1837..2346)
                     /gene="ruvC"
                     /locus_tag="VFB89_11430"
     CDS             complement(1837..2346)
                     /gene="ruvC"
                     /locus_tag="VFB89_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HZM27962.1"
                     /translation="MKVLGIDPGTAVLGFGVVESVAGRSARLVECGILTTRPRDSLPS
                     RLRIIHDGVAALLARHRPDMVAVESVFYARNARTTMVMSHARGVVLLAAEEAGVPVTE
                     YTPAMVKKTVVGRGAALKPQVGYMVAQLLRLREAPAPADAADGVAIALTHLVRAGRPP
                     GGRALLGAR"
     gene            complement(2360..3109)
                     /locus_tag="VFB89_11435"
     CDS             complement(2360..3109)
                     /locus_tag="VFB89_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZM27963.1"
                     /translation="MAGHSKWKQIKRKKAVTDARRASAWTKIIREITVAAKAGGGDPN
                     GNPRLRLAIDSARAVNMPKENIERAVKKGTGELGGAAFEELTYEGYGPGGAAIFIEAT
                     TDNANRTVAEIRHAFSRSGGNLGASNSVAWMFERKGQIYLDAGQLDEDTTLEAALDAG
                     AEDFSRDGEQYIVTTTPGAFHAVQDALRSRGIAIESAELAMVPKNTVKVEGPDAEKIL
                     KLVESLEELEDVSKVFSNFDIDAAQLAEAEA"
     gene            complement(3158..4033)
                     /locus_tag="VFB89_11440"
     CDS             complement(3158..4033)
                     /locus_tag="VFB89_11440"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HZM27964.1"
                     /translation="MSELRRPGRRRAATPLEALVARIDAAGADNPSPDTVASGFPSLD
                     RTLGSGFRRQDLIVLCGDVGSGKSAFGLGLALRAARLGTPTLVLSGEMGEERLRERAL
                     AIEGRVSIDELRQGRLNEASRAAVGAAAVAQRDLPLRLAALLEPGSGEVEAALEEIPR
                     RGLVVLDYLQLLHPTGAAPRQDERAAVAMRWLKSLAVRSNVALVALAQLPAHRRERPD
                     PRPSLDDLGARGAVKQHADIVLALYREEMYRPGHGVEGATELMLLKNRNGPTGFVDLF
                     FHQKWLRFEDLLDRD"
     gene            complement(4026..4412)
                     /locus_tag="VFB89_11445"
     CDS             complement(4026..4412)
                     /locus_tag="VFB89_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27965.1"
                     /translation="MSGAHVSWAAPLLILLPRAARGPRRDGVFALWLTVRVAQDLFAD
                     PAPPDRAHRRRVAALGHRVSSLTLPPPLKRALTAALGELERGNQLAAAQALSQLVAPA
                     REGAGPDAGDALAQAARAARTALRHE"
     gene            complement(4394..5419)
                     /gene="waaF"
                     /locus_tag="VFB89_11450"
     CDS             complement(4394..5419)
                     /gene="waaF"
                     /locus_tag="VFB89_11450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HZM27966.1"
                     /translation="MNLIVVSPHILAVRLGSLGDVLLTTPLFRVIRQRHPQVRLTVLT
                     RRRYAPLLADNPRVDDVVGLAPGDSLLGVASRLRATRFNYLLDLENSPTSWLLRVLVP
                     GRWRSSPQYRLAGQILVRTKRNHYPEDVTVAERYFDAARDLDIVPDGGPPEFFLNPEI
                     EQQTQAWLAQANVGKERPFVAFAPGASVATKRWPLEYWIKLVRRVVATGADAVMVGGP
                     DDTMLAAEVAARSSARAVNAAGVLSLQGTGALLKRAAALVTGHTGAMHIASAVGTPLV
                     ALFGPTVRAFGYHPYNSSRAFVLERNLSCRPCSVGGGAECPLGHHLCLQEIQPDAVYT
                     ALCRALT"
     gene            5515..7080
                     /locus_tag="VFB89_11455"
     CDS             5515..7080
                     /locus_tag="VFB89_11455"
                     /inference="COORDINATES: protein motif:HMM:NF020249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZM27967.1"
                     /translation="MARPKSILWVDDEVESLTSHILFLQEQGFAVETATHGDDAIVLL
                     QRQNYGVVLLDEQMPGRRGLELFHSIRAMDNALPVVMVTKSEEPDTLRDAIGAEVSDY
                     LTKPVNPRQILSVVTRLLEGDRIRQQRLSRDFTTRFRELESRRHAPMAWREWVELVVE
                     LAEWEVRLGEADEPGLQDALQTLQDSIREDFASYLRKHYASWLHDARIDRPPLSVDIG
                     AEFLRPLLDSHRRAMLVVVDCLRLDQWAMLRPIIARRFDVESAHYFSILPTATPFARN
                     SIFSGLFPAEIAARYPRWWVEQDEASLNAHEAELLVEQLREVCGRNVPVRYEKVFTSS
                     DGESLLKRLPGHLAQDGVTALVFNFIDQLTHGRTENLTLYEVARDASSLRSLTRTWFE
                     RSPLWDALREAERRGVPVLLTTDHGSIHCHTPATVYAKRDTTSNLRYKFGDDLKAQDP
                     EAALTVEDLPSFGLPARAPGTRLLLATGDRFFVYPTKLREYQARYRGAFLHGGVSPEE
                     VVLPIALLTPRRG"
     gene            7080..8120
                     /locus_tag="VFB89_11460"
     CDS             7080..8120
                     /locus_tag="VFB89_11460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZM27968.1"
                     /translation="MTPSFNARVLLVAAAGADCFPHAPLHRRALERLGCEVELIDLDY
                     GHGWGRLLGLPTLAPRLRRALARQEPELVVVLDELPLQPEVIAALRRVSGARWALWAT
                     GETPPSRRHRLEQVSSCFDALFVASTVARTDLGSNGGAPVIYLPDGCDPSVCRPMRSR
                     DQFRANVVFAGRATPHRERLLAGLAEFGLAVWGPGWRRTQLRDYCRGERLGIGDYVRA
                     YAGSTVAMNLHREDPAIAGEGCNRRLFEIAAIGVPQVAEQCADLALHFIPDREVMVGR
                     DERELKELVRASLHDGTTAEAIAAAGRRRALTEHTLMHRLAVLLQVVRGARDRQPTVI
                     DTPETSPYTLQR"
     gene            8142..9329
                     /locus_tag="VFB89_11465"
     CDS             8142..9329
                     /locus_tag="VFB89_11465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZM27969.1"
                     /translation="MPSDLLLTLAFPPMGGGVARWMYEIARRYPAPGLVVSTASHPDA
                     ADVDGTLPQPVDRLPISAKRLKTLQGLMLWARRSAVLARSHRARFTWCGDIHPAGYPA
                     KWTLERVGTPLGILLHGPDLLVLQHQVHRSAMKRRSASALLGAAAVLVANSEWTRGLA
                     QSLLRELELEPAGDRIRVVPLGTDPAFFRPGESTAGVQERYGLEEGRWIVSVSRLAAH
                     RGIDVVLRALALLDDREADVRYAVAGTGDARRELEQLAAELGLDRRVRFLGGVAEGDL
                     PGLYNLAEVYVAPARRAPLVGEGFGLALAEASSAGRPVIAARAGGVPEAVRDGQTGLL
                     VDPHRPSELADALRRLLRDRTLARKLGTAGRREIERYYNWDRVASDLAQIAADAAGVG
                     TAT"
     gene            complement(9347..10282)
                     /locus_tag="VFB89_11470"
     CDS             complement(9347..10282)
                     /locus_tag="VFB89_11470"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZM27970.1"
                     /translation="MKQATQFSLVLSGGGLKGLAHIGVFRALEERGLVPDLVVGSSMG
                     SLIGAAWACGMSAEKMTARALRVERRHVFRVAHTDMALRRMMSPAVYRREPLETLIRS
                     IVGTCTFGDLKHRLLVNTVDLASGRQCVWGNPETRDARLTDVVFASCALPGIFPPHEI
                     NGRRYVDGAIIENLPVQVAASTAPWPIIAVNVAATSVERSGPELAGFAATYIRGLEIV
                     MQTQIEASLRKWGGPPLVLIQPPVAAISMFAFDKTPELIAAGYEATRDALELLGGRLD
                     QIAEAPHPRWPPADERQVRPSTAFAVLPAQPRTAA"
     gene            10469..11641
                     /locus_tag="VFB89_11475"
     CDS             10469..11641
                     /locus_tag="VFB89_11475"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZM27971.1"
                     /translation="MADLVTAAFSPIALRTSEFPWTSDVIYLNAAGIGPLPERSRRAL
                     EEFAALRAAPHRISERHLFGTLAESRRLAAALINADTAEIALAQNTSYGVNLAARALP
                     LELGDIILLSDREFPANVYPWLAQRDRGAEIEIAPTTTEGWPDEAYMLQRMAHPRVKV
                     LAVSLTQFANGYTVDLGGLSRVARMTGTYLAVDAIQAVGQMPVDVQRTPVDFLACGGQ
                     KWLLSPWGSGFVYVRRELIPKLAPADVGWLAFEGTDDLSRLTAYDTTLRSDARRYELV
                     TLPYQDFAGFNASVGLLLEVGIEAIRDYLRAVIQPVLSWAASAGVRVASPQGVHGSAI
                     VCLDLPDAPRAYRALKKEGIVATVREGVVRLSPHLYNTPAELERVAEALDTVTAGT"
     gene            11670..>13052
                     /locus_tag="VFB89_11480"
     CDS             11670..>13052
                     /locus_tag="VFB89_11480"
                     /inference="COORDINATES: protein motif:HMM:NF017669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HZM27972.1"
                     /translation="MNFADMLRAGYGFSDPSIVLGAALDGTNVVQEPKVRIPVAMMNR
                     HGLIAGATGTGKTRTLQLLAEQLSAAGVPVFAADVKGDLSGIAQPGEANDRVSGRAKE
                     VGYNWKPLGCATEFVSLTGKLGVPLRATVSSFGPLLLAKVLDLNDTQSSVLAMVFKYC
                     DDNRLPLLDLSDLKAVLQYLSSSEGKSALAGYGGMSAASVGVLLRKMVELGAQGAEPF
                     FGEPEFDVKEFLQTTSDGRGVVTCLELADVQDKPRLFSTFLMWLLAELYHNLPEAGDL
                     PKPKLVFFFDEAHLLFDDASKAFMEQVQQVIRLIRSKGVGVFFVTQTPRDVSDDVLAQ
                     LSNRVQHALRAHTPNDEAALKATVRTFPKTTFYDLEETLTSLGIGEAVVTVLDGRGVP
                     TPVVATRLVPPASRMAGLTPDEMQADVRQSPWLAQYGQSVDRESAREMLASRMERTTP
                     PAPASPVPAGG"
BASE COUNT         2154 a         4317 c         4261 g         2320 t
ORIGIN      
        1 cgcagcggcc gcgcggcgtc cgctccatga atccctgctg gatcaggaag ggttcgtaca
       61 cttcttccag cgtgcccgcg tcttcgccca ctgctacggc caccgtgttg aggcccaccg
      121 gcccgcctcc gaacttctcg atgatggtga gcaggatccg tgcgtccatg tcgtccaatc
      181 caaactcgtc cacgttgagt cggctcagcg catcgttcga aaccggcccg gtgataacgc
      241 cgccggcgcg gacttgcgcg taatcacgca cccgccgcaa gagccgattc gcgactctgg
      301 gggtcccgcg acttcggcgg gcgatctcga gagtgccttc ctcatcgatg ggaaccttga
      361 ggatctgcgc cgagcgagtg acgatggtgc gcaggtcttc ggtagggtag aaacccaggc
      421 gttccacaat cccgaaacgc gcccgcatcg gcggcgtgag ctgcccgaac cgggtagtcg
      481 cgccgatcag ggtgaagcgc tcgagactca ttgggatggt ttgggcgtgg gggccgtccg
      541 cgatccggac atccacccgg aagtcctcca ttgccgagta gagatattcc tccagcgctg
      601 gacgcaggcg gtggatctcg tcgatgaaga gcatgtccct ggcctgcaag cccgtgagta
      661 agcccaccag gtcgcctggc ttctccagca ccggccccga cgtcgtgcgg agctgcacgc
      721 ccatctcgcg cgccatcagc atcgccagcg tggtcttacc cagtcctggc gggccgaaaa
      781 agaggatatg gtcgagcgtg tctttgcgcg cccgagcagc ctcgaccgct atctgaagcg
      841 atgctttgac ctgcgcctga ccgacgaact cgtccagccg cgacggccga agcgccgcat
      901 cggcggcggg ctcatcggga agagcagcgg gagtggtgat ttgaatttgg gccattggac
      961 ctcgactgcg tgaacggtaa acggtgaacg gtgaacggga cattccgcgc gacgggatcc
     1021 cccgtttacc gtttaccgct cacattgtac cgctgtccat cggacaatcg gccggtcgcc
     1081 gggctcgagg tcctgtaccg ccccgcagtg gttgcaccgg tccgtggttc gagccgtgcc
     1141 gagtgggacc agcttgcggt tcgtggtgcc gcagcgaggg caattccatt cggcggtcac
     1201 tgccggcaag gtcatcgggg gtggtctcca ccagcctgtt tcattctcac ttgctgccgc
     1261 cggtcagtgc tgccagtgcc cgtcgaacaa cggcggccgt gtcgccggtc gcaccgtttg
     1321 cgacgacgct tcgcaccgct tcttcggccg cagccggggc atatcccaac gcaagcaacg
     1381 cgcgcactgc atgttccgaa ccggtggacg gccgctcggc cgggccgacc gacacatcgt
     1441 cgaagcggtc ccgaagctcc agcacgagtc gctcagcctt ctttcggcca ataccgctta
     1501 ccgtgctgag tgccgcaata tctcgatcgc gaatactgcg gacagtgcga tccggcccca
     1561 gcgccgacag cacggccagg gcgagcttcg gaccgaagcc gcttgcgccg agcagccgct
     1621 ggaagattac tcgatcggtt ggtcggtcga acccgaacag ggcccagccg tcttcgcgga
     1681 cgaccaactc ggtgtacaag ttgacgcgag cgccgcgcgg gggcagccgc tcgaaaacgc
     1741 cgagcgggac cgtgacggca tagccgacac caccgtcggt ctgcaccacg accgtctcgc
     1801 cctcgcgatc tgcaaggacg cccgcgatgc tcgctatcat ctcgcgccca ggagcgcgcg
     1861 ccctccgggg ggtcggcccg cccggaccag gtgcgtcagt gcgatcgcga cgccgtcggc
     1921 cgcgtccgcc ggcgctggcg cctcacgcag ccgaagcagc tgcgccacca tataacccac
     1981 ctgaggcttg agcgcggcac cgcgccccac caccgtcttc tttaccatgg cgggagtgta
     2041 ctcggtgacc ggcacgccgg cttcctctgc cgccaacaac actacaccgc gcgcatggct
     2101 catgaccatg gtcgtgcgcg cgttgcgggc gtagaacacg ctctctaccg ctaccatatc
     2161 gggtcgatgg cgggcaagca gggcggcgac accgtcgtgg atgatgcgca aacgggaagg
     2221 gagcgagtcg cgcgggcggg tggtcaggat gccgcactcg accagccggg cggagcggcc
     2281 ggcaaccgac tccaccaccc cgaagccgag gacggcggtg ccggggtcga tgccgagcac
     2341 cttcatgcgg ccggccggat caggcctctg cctcagccag ctgtgccgcg tcgatatcga
     2401 agttcgagaa aacctttgag acgtcttcca gttcctcgag gctctcgacc agcttgagga
     2461 tcttttcggc gtcgggccct tccaccttca ccgtgttctt cggcaccatg gccagctccg
     2521 ccgattcgat tgcgatccca cggctgcgga gtgcatcctg aacggcgtga aaggcgcccg
     2581 gtgtcgtcgt cacgatgtat tgctcgccat cccggctgaa gtcctcggcg ccggcatcga
     2641 gggcggcttc gagcgtggtg tcctcgtcca gttgcccggc atccaggtag atctgtcctt
     2701 tgcgctcgaa catccacgcg accgagttcg acgcgccgag atttccgcca ctccggctga
     2761 acgcgtggcg gatttccgcc accgtgcggt ttgcgttgtc ggtcgtggcc tcgatgaaaa
     2821 ttgcggcgcc tcccggccca tagccctcgt aggtgagttc ctcgaaggcc gcgccgccga
     2881 gctctccggt gcccttcttg acggcgcgct cgatgttctc cttgggcatg ttgacggcac
     2941 gagccgagtc gatcgcgagg cggaggcggg gattcccgtt gggatcgcca ccccccgctt
     3001 tcgccgcgac ggtgatttcc cggatgatct tggtccaggc cgaagcccgg cgcgcatcgg
     3061 tcaccgcctt cttccgcttg atctgcttcc atttgctgtg tccagccatc accgaactcc
     3121 ttgcggctcc cgaagttgca caaacataag ccgcggttca gtcgcggtcc agcaggtctt
     3181 cgaagcgaag ccacttctga tggaagaaga gatcgacgaa tcctgtgggc ccattccgat
     3241 tcttgagcaa catgagctcc gtggcgcctt cgacgccgtg gccggggcga tacatctcct
     3301 cgcgatagag cgcgagcacg atgtcggcgt gttgcttgac cgcaccccgc gcgcccaggt
     3361 catcgagcga cggtctcggg tcggggcgct cacgccgatg ggccggcagc tgagccaggg
     3421 ccaccagcgc cacattcgac cgaaccgcga gtgacttgag ccaacgcatc gcgacggccg
     3481 cgcgctcgtc ttgccgagga gcggctccgg tggggtgtag gagctggagg tagtcgagca
     3541 cgaccagtcc acggcgggga atctcttcga gagcggcttc gacttcaccg gacccgggct
     3601 cgagcagtgc ggcgagcctg agcggcagat ctcgctgggc caccgccgcc gcccccaccg
     3661 ccgcgcggct ggcctcgttg agccgcccct gtcgcagctc atcgatcgag acgcggccct
     3721 cgatggcaag ggcacgctcg cgcagacgct cctcacccat ctcccccgaa agcaccagag
     3781 tcggcgtgcc gagccgagcg gcgcgcaggg cgagaccgag cccgaaagct gatttgccgc
     3841 tgccgacgtc gccacacagg acgatcaggt cctggcgacg gaagccgctg cccaacgtcc
     3901 gatcgaggga agggaagccc gacgcgacgg tgtcgggcga agggttatcc gcgccggcgg
     3961 cgtcgatccg cgcaaccagc gcctcgagcg gcgtcgctgc gcgccgtctg cccggccgac
     4021 gtagctcact catggcggag cgccgttcga gctgcccggg cagcctgtgc caatgcatct
     4081 cccgcatccg gcccagcgcc ttcacgggcc ggcgccacca gttgactcag cgcctgagcc
     4141 gccgcaagct ggttgccgcg ttccaattcg cccaatgccg cggtaagcgc gcgtttgagc
     4201 ggcggcggaa gagtcagact cgaaacccgg tgcccgagcg cggcaactcg cctccgatgg
     4261 gctcgatcgg gtggcgccgg gtcggcaaaa agatcctggg cgacccgcac ggtaagccag
     4321 agagcaaaca cgccgtcgcg ccgaggtccg cgggcggctc gggggagcag aatcaggagc
     4381 ggtgcagccc agctcacgtg agcgcccgac atagcgctgt gtatacggcg tccggctgaa
     4441 tctcctgcag gcacaaatgg tggccgagcg gacattcggc gccgccaccg acactgcacg
     4501 gacggcacga caggttgcgc tccagcacaa aggcccggct cgagttgtac ggatggtaac
     4561 cgaacgctcg gacggtcggt ccgaacagcg ccaccagagg cgttcccacc gccgaggcaa
     4621 tgtgcatcgc tccggtgtgt ccggtgacca gcgcggcggc tcgcttgagc agcgcgccgg
     4681 ttccctggag cgacaggacg ccggcagcgt tcaccgctcg cgcgctgctc cgtgcggcga
     4741 cctcggcggc gagcatggtg tcatccggcc cgccgaccat gacggcatcc gcaccggtcg
     4801 cgaccactcg gcggaccagc ttgatccagt attccagggg ccagcgcttg gttgccaccg
     4861 atgcgcccgg agcgaacgcg acgaacggcc gttccttccc gacgttcgcc tgggcgagcc
     4921 atgcctgggt ttgctgttcg atttccggat tgagaaagaa ctcaggtggg ccaccgtcag
     4981 gcacgatatc gaggtcacga gcggcatcga agtagcgctc tgcgacggtg acgtcttccg
     5041 gataatgatt gcgtttggtg cgaacgagga tctgtcccgc cagacgatac tgcggagacg
     5101 agcgccaccg tcccgggacc agaacgcgta gtagccagct cgtcgggctg ttctccaggt
     5161 cgaggaggta gttgaaccgg gtggcgcgca gccggctggc aaccccgagc agtgagtccc
     5221 ccggtgcgag ccccacaaca tcatcgacac gtggattgtc ggctagcaga ggtgcatagc
     5281 ggcggcgggt gagaaccgtc agcctaacct gcgggtgccg ctggcggatg acccggaaca
     5341 gcggtgtcgt cagcagcacg tcgcccagcg agcccagccg caccgcgagt atgtggggag
     5401 agacaacaat caggttcact tggggaatgt acggaccgcc tggaggtggt gccagcggcc
     5461 aagtccttgg gctgccgcgt tgtgcgcctc tctccgctgc tctacttttc gcctatggcg
     5521 cgccccaaga gcattctctg ggtggacgac gaggtcgaga gcctcacgtc ccacattctt
     5581 tttttgcagg agcagggctt cgcggtcgag accgccactc acggtgacga tgcgatcgtg
     5641 ctgctgcaac ggcagaacta cggggtggtg ctgctggacg agcagatgcc cggccgcagg
     5701 gggctggagc tgttccattc gatccgcgcc atggacaacg ccctgcccgt cgtgatggtc
     5761 accaagagtg aagagccgga cacgctgcgc gatgcgatcg gcgcggaggt ttccgactac
     5821 ctgaccaagc cggtcaatcc tcgtcagatc ctttcggtcg tcacccgctt gctcgaaggc
     5881 gatcgcatcc gtcagcagcg gctctcccgt gacttcacca cccgcttccg cgagctcgag
     5941 tcgcgtcgac acgcaccgat ggcctggcgc gagtgggttg agctcgtcgt ggagctggcc
     6001 gaatgggaag tgcgcttggg cgaggcggac gaacccggct tgcaggacgc gctacagact
     6061 cttcaggaca gtatccggga ggattttgcc agctacctca ggaagcatta cgcgagctgg
     6121 ctgcacgacg cgcgcataga ccggccgccg ctctcggtgg atatcggcgc ggaatttctg
     6181 cgtccgctgc tcgacagcca ccgacgtgca atgctggtgg tggtggactg ccttcgcctg
     6241 gatcagtggg ccatgctccg gccgatcatt gcccggcgct tcgatgtcga gagcgcgcac
     6301 tacttctcca tcctgcccac cgccaccccg tttgcccgga attcgatctt cagcgggctg
     6361 ttcccggccg agatcgcagc ccggtatccc cgctggtggg tggagcagga tgaagccagc
     6421 ctgaatgcgc acgaagcgga gctgctggtg gagcagttgc gcgaggtgtg cggccgcaac
     6481 gtgccagtgc gctatgaaaa ggtcttcacc tcgtccgatg gtgagagcct gctcaagcgt
     6541 ttgcctggac atctggccca ggacggcgtc acggcgctgg tcttcaactt catcgaccag
     6601 ctgacccacg gacgaaccga gaacttgacg ctctatgaag tcgcccgcga tgcctcatcg
     6661 ttgcgaagcc tgacgcgaac ctggttcgag cgctctccac tgtgggacgc gctcagggaa
     6721 gccgagcggc gcggtgtccc ggtcctgctc acgaccgacc acggctcaat ccactgccat
     6781 acccccgcca ccgtgtacgc caaacgcgac actacgtcga accttcgcta caaattcggc
     6841 gacgatctga aggcccagga cccggaggcc gccctcaccg tggaggacct gcccagcttt
     6901 ggccttcccg cccgggcgcc ggggacccgc ctcttgcttg ccacgggtga ccggttcttc
     6961 gtgtatccca ccaagcttcg cgagtaccag gccagatatc gcggcgcttt tcttcacggc
     7021 ggtgtttccc cggaggaagt cgtgctgcct atcgcattgc tgacgccgag gcgcggatag
     7081 tgacgcccag cttcaacgcc cgggtgctcc tggttgccgc cgccggcgcc gactgcttcc
     7141 ctcacgcccc gctccaccgc cgcgcgctcg aacggctggg gtgtgaggtc gagttgatcg
     7201 acctggacta tgggcacggc tggggtcggt tgctcggttt gcccacgctg gccccacgcc
     7261 tgcggagagc gctggcccgc caggaaccgg agctggtggt agtcctggac gagcttccgc
     7321 tccagcccga ggtgatcgcg gctctgcgac gagtttcggg cgcgcgttgg gcactctggg
     7381 ccaccggaga aacgccgccg tcccggcggc accgcttgga gcaggtgagt tcctgtttcg
     7441 acgccctctt cgttgcatcg accgttgccc ggaccgatct tggctcgaac ggtggagcgc
     7501 cggttatcta cctgcccgat gggtgcgatc cgtcggtatg ccgaccgatg cgttcgcggg
     7561 accagttccg ggccaacgtc gtgttcgcgg gtcgggcgac gccgcatcgg gagcggttgc
     7621 tcgcggggct ggcggagttc ggtctcgcgg tgtgggggcc tgggtggcgc cggactcagc
     7681 tccgcgatta ttgccggggc gagcggctcg gaatcggtga ctacgtccgc gcctatgccg
     7741 gctctaccgt cgcgatgaac ctgcatcggg aagacccggc catcgcaggc gaaggctgca
     7801 atcggcgcct tttcgaaatc gcggccatcg gcgtgcccca ggtcgccgag cagtgcgctg
     7861 acctcgcgct gcacttcatc cctgatcggg aggtgatggt tgggcgggac gagcgggagc
     7921 tgaaggaatt ggtccgggcc tcgcttcacg acgggacgac cgccgaggct atcgcggccg
     7981 ccggacggcg ccgtgctctt acggaacaca cgttgatgca tcggttggca gttttgctcc
     8041 aggtagtgcg cggtgcacgg gaccgccagc cgacggttat cgacacccca gaaacttcac
     8101 cctatacact gcagcgctga cggctcgact cgccatctct cgtgccctcc gacctgctgc
     8161 tgacgttggc atttccgccg atgggcggtg gggtggcgag atggatgtat gagatcgctc
     8221 ggcgctaccc cgcccccggt ttagtcgtct cgaccgcgtc gcaccccgat gccgcggacg
     8281 tcgacggaac gcttccgcag ccggtggatc gtctaccgat ctctgccaag cgcctcaaga
     8341 cccttcaagg actcatgctc tgggcccgtc gctcggcggt gctggcccgc tcccaccgag
     8401 cccggtttac ctggtgcggc gatatacatc ccgcaggcta tcccgccaaa tggacccttg
     8461 aacgggtagg aactccattg ggcatcctgc ttcatgggcc cgatctcctg gtgctgcaac
     8521 atcaggtaca tcgatcagca atgaagcgcc ggagcgccag tgcgttgctg ggagcggctg
     8581 ccgtcctggt agccaacagc gagtggactc gaggcctggc ccagtcgctg ctgcgcgagc
     8641 tcgagctcga gcccgctgga gatcgaatcc gtgtggttcc gctcgggact gatcccgcct
     8701 tcttccggcc gggcgagtcc accgctggtg ttcaggagcg gtacgggctc gaagagggcc
     8761 gttggatcgt gtcggtatcg cgactcgcgg ctcatcgggg catcgatgtc gtgctgcgcg
     8821 cactggcgct cctcgacgac cgcgaagccg atgtgcggta cgctgtcgcc ggtaccggcg
     8881 atgcccgccg tgagctggag cagctcgccg cggagcttgg cctggatcgc cgcgtccgct
     8941 ttctgggcgg cgttgccgag ggtgaccttc ccgggctcta caacctggct gaggtctatg
     9001 tcgcccctgc ccgccgagcc ccgctcgtcg gggaggggtt tggactcgca ctggcggagg
     9061 cctcgagcgc cggcagaccg gtaatcgccg ctcgggcggg aggtgttccg gaagccgtgc
     9121 gcgatggaca aaccggtttg ctggtcgacc cccaccgccc gtcagagttg gccgatgccc
     9181 tacgacggct gctacgggat cggacgctgg ctcgaaagct cggtacggcc ggtcgacggg
     9241 agatcgagcg ctactacaac tgggataggg tcgcgtctga tctggcacag attgctgcgg
     9301 atgcggcggg cgtcggaacc gcaacgtagc gcccgggtct ctcagctcag gccgcggtcc
     9361 gtggttgtgc aggcagcacg gcaaaggccg tagacggccg gacctgccgt tcgtcggcag
     9421 gcggccagcg ggggtggggc gcctcggcga tttggtcgag ccgaccgccg agcaactcga
     9481 gggcgtcccg ggtcgcttcg tatcccgccg cgatgagctc cggagtcttg tcgaaggcaa
     9541 acatcgagat ggcggccacc ggcggctgga tcagcaccag cggcggacca ccccacttgc
     9601 ggagcgaagc ctcgatctgg gtctgcatta cgatttcgag cccgcgaatg taggtcgccg
     9661 caaacccggc caactcgggc ccgctacgct ccacactggt ggctgcgaca ttcaccgcga
     9721 ttatcggcca gggagccgtc gaagccgcca cttgaaccgg caggttttcg atgatcgccc
     9781 catcgacgta gcgtcgtccg ttgatttcgt gcggcgggaa aatgccggga agcgcacagg
     9841 aagcgaaaac cacgtcggtc agtcgcgcgt cgcgtgtttc cgggttgccc cagacgcact
     9901 gccgaccgga tgccagatct accgtattta ccaggagccg gtgcttgaga tcgccgaagg
     9961 tgcaggtacc gacaatgctg cggatcagcg tttcgagcgg ttctcggcga tagacggccg
    10021 gcgacatcat tcgccggagc gccatatccg tatgggcgac tctgaagaca tggcgtcgct
    10081 ccacccgcaa cgcccgggct gtcatctttt cggccgacat cccgcaggcc caggcggcgc
    10141 cgatgagcga tcccatgctc gagcccacga cgagatcggg cacgagtccc cgttcctcga
    10201 gagcgcgaaa caccccaata tgggcgagac ccttgaggcc gcctccgctc aaaaccaggg
    10261 aaaattgggt cgcttgtttc acctgcttgc ctcgaccgag taccccgccg taacctttgc
    10321 aatccgggtg ccagaggggg cccggcgtgc gaccatgcca aaatctgcag tgtccttacc
    10381 ccggagggga caaacgcggg ggcgcgacgt cctgaggtgg ctacctcccc ggggatggcg
    10441 ttgacctatt accagcgttt aatggcccat ggctgacctt gtgaccgcgg cgttttcccc
    10501 catcgctctc cggacgagcg aatttccctg gaccagcgac gtcatctatc tcaatgcggc
    10561 cggcattggg cccctgcccg agcgatcccg ccgggcgctc gaggagttcg ctgccctcag
    10621 ggcggcgccc catcggatct ccgaacggca cctgtttgga accctggcgg agtcccgccg
    10681 ccttgcagcg gcgttgatca acgccgacac agctgagatc gccctggccc agaacacgag
    10741 ctacggcgtg aacctcgccg ctcgggctct gccgctcgag ctgggcgaca tcattctgct
    10801 gagtgatcgc gagtttcccg cgaacgtcta cccgtggctc gcgcagcggg atcgcggagc
    10861 cgaaatcgaa atcgcgccga ctaccacgga agggtggccc gacgaagcct acatgctcca
    10921 gcggatggcg catccgcgcg tgaaagtgct cgcggtgtcg ctcacccagt tcgccaacgg
    10981 ttacacggtg gaccttggcg gcctctctcg agtggctcga atgaccggga cctacctcgc
    11041 agtcgacgcg atccaggcgg tggggcaaat gccggtcgac gttcagagaa ctccggttga
    11101 cttcctggcg tgcggaggcc aaaagtggct cctctcgccc tggggatccg ggttcgtgta
    11161 cgtgcgtcgg gaactgattc ccaagctggc gcctgcggat gtcggttggc tcgccttcga
    11221 gggcacggac gacttgagcc gactcacagc ctacgacacg acgcttcgct ccgatgctcg
    11281 tcgctacgag ctggtgacct tgccgtatca ggacttcgca ggcttcaatg cttcggtagg
    11341 tctgctgctc gaggttggga tcgaagcgat acgcgactac ctgcgagcgg tgattcagcc
    11401 cgtcctttcc tgggcggcga gtgccggcgt acgcgtagct tcgccccagg gtgtccacgg
    11461 atcggccatc gtttgtttgg acctccccga cgcaccccgc gcttatcggg cgctcaagaa
    11521 ggaggggatc gttgccacgg tgcgggaagg tgtggtgcgc ctgagtcccc atctctacaa
    11581 cacgcccgcc gagctggaaa gggtggcgga agcgctggac accgtaacgg ccgggacgta
    11641 acctcagtca tctcccggca tcgattcgta tgaatttcgc cgacatgctt cgggcgggat
    11701 acggattctc cgatccttcc attgtgttgg gcgccgcgct ggatggcacc aacgttgttc
    11761 aggagccgaa ggtccggatc cccgtcgcga tgatgaaccg ccatggcctc attgccgggg
    11821 ccaccggcac aggaaagact cgaaccctcc agttgcttgc ggaacagctc tccgcggcgg
    11881 gcgtgcccgt atttgctgcc gatgtcaagg gcgatctctc cggcattgca cagcccggag
    11941 aggcgaacga tcgggtcagc ggtcgcgcca aggaggtcgg gtacaactgg aagcctctcg
    12001 gttgcgccac cgagttcgtg agtctcacgg gaaagctcgg cgttcccctg cgagccacgg
    12061 tgagctcgtt cggaccattg ctcctggcca aggtgctcga cctcaacgac actcagtcga
    12121 gcgtgctggc catggtgttc aagtattgcg acgataatcg gcttccactg ctcgatctct
    12181 cggacctcaa agctgtgctt cagtacctga gctcgagtga gggcaagagc gccctggccg
    12241 gctatggcgg tatgtcggcc gcgtcggtcg gagtgctgct tcgcaagatg gtagagctcg
    12301 gtgcgcaggg ggccgagccg tttttcggcg agcccgagtt cgatgtgaag gaattcctcc
    12361 agacgacatc ggatggacgt ggcgtagtca cttgcctcga gctggcggac gtgcaagaca
    12421 agccccgact cttctcgact ttccttatgt ggctcctggc cgagctgtac cacaatctgc
    12481 ccgaggccgg tgatctgccg aagccgaagc tcgtgttctt cttcgatgag gcccacctgc
    12541 tgttcgatga tgcctccaag gcgttcatgg agcaggtgca acaggtcatt cggttgatcc
    12601 ggtccaaggg cgtcggcgtg ttcttcgtca ctcagacacc cagggatgta tccgacgatg
    12661 tgctggccca gctctcgaac cgggtacagc acgccctccg ggcccacacc ccgaatgacg
    12721 aagccgcgct caaggccacg gttcgaacct ttcccaagac cacattctac gatctcgagg
    12781 aaacgctgac ctctctcggt atcggcgaag cggtggtgac ggtgctcgac ggccgcggag
    12841 tgcccacacc ggtggttgcc acgcgccttg tgccgccggc atcccggatg gcgggattga
    12901 cgcccgacga gatgcaagca gatgtacggc aatcgccctg gctggctcag tacggccagt
    12961 ccgtcgaccg ggagagcgcg cgggagatgc tggcgagccg gatggaacga accaccccgc
    13021 ccgcaccggc gagccccgtt cccgccggcg gt
//
LOCUS       DASRJQ010000500         4594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3412579, whole genome shotgun sequence.
ACCESSION   DASRJQ010000500 DASRJQ010000000
VERSION     DASRJQ010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3412579"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VFB89_11485"
     CDS             complement(<1..521)
                     /locus_tag="VFB89_11485"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:NF014857.4"
                     /note="catalyzes the formation of
                     N(1)-(5-phospho-D-ribosyl)glycinamide from
                     5-phospho-D-ribosylamine and glycine in purine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HZM27973.1"
                     /translation="MRILVVGGGGREHAICWTLRRENPDADIHCAPGNPGTALVAHNL
                     PIPSEDIDRIADAADMLGIDLTIVGPEIPLALGLADRLRAEGRAVMGPSAAGAQIEAS
                     KAFAKEIMAAAGVPTASSGTFTDLPSALTYVDQHAEPLVVKASGLAAGKGAVVCGTRK
                     EAAQTVRAMLGDGA"
     gene            complement(518..1531)
                     /locus_tag="VFB89_11490"
     CDS             complement(518..1531)
                     /locus_tag="VFB89_11490"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HZM27974.1"
                     /translation="MTSVPTTRPRIRFPEIAGVSWEHPADRAALQSLRAVPGFDDLVR
                     KVISFLGGERGIRLLFQGNAVRASGVQFPKLWSLQTEVAGTFDWPKVPELYVSQTPVF
                     NAGAYGVDDPFIVVHSAALEMLDDDELRVLLSHEMGHVVSGHALYRTIAEILLSVGLA
                     ALPILAGIALFPIRLAILEWARKAELSADRAGLLGSQDVVASQRLFMKMAGGTRGQIN
                     TGQLSVDAFMVQANEYLASREGFDAIYKILNTLALTHPMNTIRAAELQQWVASGEYDR
                     ILRGEYVRRGSEAASRPLVDDLSAAGSHYAAEAREFASAVADAAKRAAERAAEAFRSA
                     QRR"
     gene            complement(1679..2851)
                     /locus_tag="VFB89_11495"
     CDS             complement(1679..2851)
                     /locus_tag="VFB89_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HZM27975.1"
                     /translation="MTRARTQDTDLGFATRAIHAGQDPDPLAGAVMPPIYQTSTYVQE
                     SLGRHKGYEYARTQNPTREALERNVASLEGAAHGFAFGSGLAALDALLKLFKAGDHVV
                     CGENVYGGSMRLMMRVYAELGLQFTFVDMRDIGPIERAITPRTRLVFCETPTNPMMFL
                     TDLAAVGDLTQARGLLYAVDNTFATPFFQRPLAFGADIVLHSTTKYLNGHSDMVGGLL
                     VTNRDDLAERLGFLQNAAGAVPGPMDCWLALRGLKTLPLRMRQHDASGRRIAQWLTTR
                     KEVTRLYYPGLPSHPQHELACRQMTGFGGMISFDLGDTGRVRRFVEKLRIFALAESLG
                     GVESLIGHPASMTHAAVPPALRQEMGLTDSLLRLSCGCEDPEDLIADLEQAFDGTA"
     gene            2951..4000
                     /locus_tag="VFB89_11500"
     CDS             2951..4000
                     /locus_tag="VFB89_11500"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZM27976.1"
                     /translation="MTSSSQRNSGAARCLVVDDDPQVRGALVRVIQSQGLSCVEASSG
                     TEALDLLRRDGEIPVCISDIYMPEMDGVTFLRQALELYPDMAIIMLTGVAEVSTAVEC
                     LQIGALDYISKPVLIDEVKARVEKALEKRDLILQNRFYQQHLESRVRELDRLNKQSLI
                     NGVQMLVHALEAKDAYTSGHSARVSKYAVKTAVILGITGERIEQIRLGGELHDIGKIG
                     TREAVLNKPGPLAPEEFEHIKIHVTLGERILAPFLGESPGVLQIVRSHHERMDGSGFP
                     DHLRASEIPIEARIVSVVDAFDAMTTNRAYRPCRTPGEAVDELRGHAGPQFDPDVVHA
                     FLTAFPDVAARPLHA"
     gene            4011..>4594
                     /locus_tag="VFB89_11505"
     CDS             4011..>4594
                     /locus_tag="VFB89_11505"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27977.1"
                     /translation="MSSTLMISVSGMRGLVGTDLTPELVARHAAALGAWAKQKNNPLV
                     VLGRDARTSGPMFARAATAGLMSTGAEVIDLGIVPTPTVQLAVEYHHAGAGLILTASH
                     NPIEWNALKFIASTGLFLDAAEANSLRALMREGVKGATWDELGFTEPDPGAVQRHIEA
                     VLRLSVIDADQIRSRRFRVALDTCHGAGSAIMPEL"
BASE COUNT          859 a         1444 c         1467 g          824 t
ORIGIN      
        1 gcgccatcac ccagcattgc ccgaaccgtt tgcgccgcct ccttccgcgt gccgcaaacg
       61 accgcaccct taccggcggc gagccccgat gccttcacga ccagcggctc ggcgtgctga
      121 tcgacatacg tcaaggccga cggaagatcc gtaaacgtgc cgctgctcgc tgtgggaacg
      181 cccgcggcag ccatgatctc cttcgcgaat gctttcgagg cttcgatctg ggcgcctgca
      241 gcgctcggtc ccatgaccgc gcgtccctca gcccggagac gatcggccaa tccgagagcc
      301 agcggaattt caggtccaac gatggtgaga tcgatgccga gcatgtctgc ggcgtcggca
      361 attcggtcga tgtcttcgga cggaatcggg aggttatgcg caaccaacgc ggtgccgggg
      421 ttccccgggg cacagtgaat gtcggcgtcg ggattttcgc ggcgaagcgt ccagcagatg
      481 gcgtgctccc gtccgcctcc gccgacgacg agaatcctca ccggcgctgc gccgaacgaa
      541 aagcttcggc agcgcgctcc gcggcacgct tggccgcatc ggcgacggcg ctcgcaaact
      601 cccgagcttc ggcggcatag tggctgcccg ccgcgctcag gtcgtccacc agcgggcgcg
      661 atgcagcttc tgacccgcgg cgaacgtact cgcctcgaag tatccggtcg tactcgccgc
      721 tcgccaccca ctgctgcagc tcggcggcgc gtatggtgtt catcgggtga gtgagtgcca
      781 gcgtattcag aatcttgtaa atggcgtcga atccctcacg actcgccaaa tactcgttgg
      841 cctgaaccat gaatgcgtct acgctcaact gcccggtgtt gatctgaccc cgcgtgcccc
      901 cggccatttt catgaacaac cgctgggacg cgaccacatc ctggcttccc agcagccccg
      961 cgcggtcggc ggacagctcg gccttgcggg cccattccag aatcgcgagc cggatgggga
     1021 aaagggcaat acctgcgagg atcggcagtg ccgcaagccc gacgctcagc aggatctcgg
     1081 cgatcgttcg gtagagtgca tggccgctga ccacgtgtcc catttcatgc gagagcagca
     1141 cccgcagctc gtcatcatcc agcatctcga gcgcggcgga atgcaccaca atgaacggat
     1201 cgtccacgcc gtatgcgccg gcgttgaaga ccggggtttg tgagacgtag agctcgggca
     1261 ccttgggcca gtcgaaggtc cctgctactt cagtctgcag gctccagagc ttgggaaact
     1321 ggaccccact cgcgcgcacc gcgttgccct ggaagagcag tcggatgccc cgttctccgc
     1381 caaggaagct gatcaccttg cgaacgagat cgtcgaagcc aggaaccgcc cgcaaagact
     1441 ggagtgcggc gcgatcggcc ggatgctccc agctcactcc ggcgatctcg ggaaaccgga
     1501 tgcgaggtcg agtggtgggg acggacgtca tggtctgctc acggctgaag gtctggcacc
     1561 gtacgtaggg cgctaagcaa tgtttcaatt tgaaaagcgt aaaaggtgaa cggtaaacgg
     1621 tgaacaggga actgcagcat gctacggaat cgcctgttca ctgttcaccg ttcaccgttc
     1681 acgcagttcc atcaaacgcc tgctccaaat cggcgatcag gtcttccgga tcctcgcagc
     1741 cgcagctcag gcgaagcaag ctgtcggtca accccatctc ctggcgcagg gcggggggga
     1801 cggcggcgtg agtcatggag gcgggatgcc cgatcaggct ctcgaccccg ccgagcgact
     1861 cggccagggc gaagatccgg agcttctcga cgaaccgccg gactcgcccg gtatcaccca
     1921 ggtcaaagga gatcattccg ccaaagccgg tcatctgccg gcaggcgagc tcgtgctgcg
     1981 gatgcgatgg aagtcccgga tagtaaagcc tggtgacttc cttacgagtg gtgagccatt
     2041 gcgcgattct gcggccgctg gcgtcgtgct ggcgcatgcg aagcggcagc gtcttgagcc
     2101 cccggagcgc cagccagcag tccatcggac ccggcaccgc acctgccgcg ttctgaagaa
     2161 atcccagtcg ttcggccagg tcgtcccggt ttgtgaccag cagcccgccg accatgtcgc
     2221 tgtggccgtt gaggtacttg gtggtcgaat ggagcacgat gtcggcaccg aacgcgagcg
     2281 ggcgctgaaa gaacggcgta gcgaatgtgt tgtcgactgc gtagagaagc ccgcgagcct
     2341 gagtgaggtc ccccaccgcg gcgaggtcgg tcaggaacat catcggattg gtcggcgtct
     2401 cgcagaacac gagccgggtt cgcggcgtaa tggcccgctc gatcggcccg atgtcgcgca
     2461 tatcgacgaa ggtgaactgc aacccgagct cggcgtagac acgcatcatc aagcgcatgc
     2521 ttccaccata gacgttttcg ccgcacacca catggtcgcc agccttgaac agcttgagaa
     2581 gcgcgtcgag ggccgccagt cccgagccga aggcgaagcc atgggctgcg ccctccaggc
     2641 ttgcgacatt acgctcgaga gcctcccggg tcgggttctg ggttcgggcg tactcatagc
     2701 ctttgtgtcg gcccagggat tcctggacgt aggtagatgt ctgataaatc ggaggcatca
     2761 cggcgccagc cagcgggtcg ggatcctgac ccgcgtgaat agcgcgggtg gcgaagccga
     2821 gatcggtatc ttgtgtgcgc gcgcgagtca tcgcaggggc cgggcagatg tcggggaagc
     2881 gtaacggctt gcgctgcatc cctgcaagcg gctggttgaa gatttcccct acggaatcta
     2941 ctttcgcgga gtgacttcct cctctcagcg caactcgggt gctgcgcgct gcctcgtggt
     3001 agacgacgat ccccaggtgc gcggagcgtt ggtgcgggtc atccagtcgc aggggctgtc
     3061 gtgcgtggag gcatcctccg gaacggaggc gctcgatctc ctgcggcggg atggcgaaat
     3121 ccccgtatgc atctcggaca tctacatgcc cgagatggac ggcgtgacct ttctccggca
     3181 ggcccttgag ctctacccgg acatggcgat catcatgctc accggcgttg ccgaggtgag
     3241 cacggcggtc gagtgtctgc agattggggc gctcgactac atctccaagc ccgtgctcat
     3301 cgacgaagtg aaggcccggg tggagaaggc gctcgaaaag cgcgatctga ttctgcagaa
     3361 ccgcttctat cagcagcacc tcgagagtcg agtgcgcgag ctcgaccggc tcaataagca
     3421 gtcactcatc aacggcgtgc agatgctggt ccacgcgctc gaggccaagg atgcgtatac
     3481 cagcggccat tcggcccgag tcagcaagta cgccgtcaag accgccgtga ttcttggaat
     3541 taccggagaa cggatcgagc agataagact gggaggggag ctgcacgata tcggcaagat
     3601 cggaacccgg gaggcggtgc tgaacaagcc cgggcctcta gctcccgagg agttcgagca
     3661 catcaagatt cacgttacgc tgggggagcg cattctcgcg ccatttctcg gcgaatctcc
     3721 cggcgtgctc cagatcgtcc ggtcgcacca cgagagaatg gacggcagcg ggtttcccga
     3781 tcacttgcgg gcgtccgaga ttccgatcga agcgcgaatc gtctcggtag tggacgcctt
     3841 cgatgcaatg acgaccaatc gtgcctaccg tccctgccgg acaccgggag aggccgtgga
     3901 tgagctgcgc ggccatgccg gaccgcaatt tgaccctgac gtggtacacg cgttcctgac
     3961 cgcgttcccc gacgtcgcgg cgcgtccact tcacgcctga tagtgcttac gtgtcctcca
     4021 ccttgatgat cagcgtgtcg ggaatgcggg gcctggtcgg caccgacctc actcccgagc
     4081 tggtagcgcg ccatgcggcc gcgctcggcg cctgggccaa acagaagaac aatccgctgg
     4141 ttgtgctcgg ccgggacgct cggaccagcg gacccatgtt cgcgcgagca gcaaccgcgg
     4201 gcctcatgtc gaccggggcc gaggtcatcg atctcgggat cgtgccgacg cccacggtgc
     4261 agctggcggt ggaatatcac catgccggcg ccggactcat tctcaccgcc agccacaatc
     4321 ccatcgagtg gaacgccctc aagttcattg catccaccgg ccttttcttg gacgctgccg
     4381 aggcgaattc gctgcgtgct ttgatgagag aaggggtgaa aggcgctaca tgggacgagc
     4441 tgggctttac ggagccagac cccggtgcgg tgcaaagaca tatcgaggca gttctgcgcc
     4501 tatcagtgat cgatgctgat cagatccgtt cgcgccggtt ccgtgtggcg ctggacactt
     4561 gtcacggagc cggctcagcg attatgcccg agct
//
LOCUS       DASRJQ010000501        10271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_587093, whole genome shotgun sequence.
ACCESSION   DASRJQ010000501 DASRJQ010000000
VERSION     DASRJQ010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 10271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10271
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587093"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="VFB89_11510"
     CDS             <1..846
                     /locus_tag="VFB89_11510"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZM27978.1"
                     /translation="ATTGGRMSGAALELIGLRHTFFPGTPSEQRALEGVSLTVDPGTF
                     VVVIGINGSGKSTLLNGVAGSVTLDEGIVRIGGRDVTRWPEHRRAGMIGRVFQNPFSG
                     TAPHLTVAENLAIASQRGRSRQLFRLGLSSGRRAQLAEQLASMNLGLEHRLVTPTGVL
                     SGGQRQALTLLMATIVRPAVLLLDEHTAALDPRSAEQVLRLTEETVARDRLTTLMVTH
                     SMHQAVRIGDRLLLVHRGRILEDFSGPRKRRLRMEDLLDRFDQLRNADMLDESAAEML
                     RRAYV"
     gene            898..1350
                     /locus_tag="VFB89_11515"
     CDS             898..1350
                     /locus_tag="VFB89_11515"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HZM27979.1"
                     /translation="MPAVPEWPAVPPRPAVVSAFWRAARLHCPACGGGPVFLSWIRIA
                     PACPSCGLNFTRGERGYWLGAYFVNLMAVEAIFAVMLGIALWWTWPTPPWEGIEWALG
                     VAMLVSPFLLYPWSHTIFLAFDLLFRPPASEDFEAPHEPAPVFRRRGR"
     gene            1488..2345
                     /locus_tag="VFB89_11520"
     CDS             1488..2345
                     /locus_tag="VFB89_11520"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HZM27980.1"
                     /translation="MAVATAPSVTNRPLAGDIDVFGLTHPGHVRRVNQDQFLIASLHK
                     TMQVHASSVPATQLGDLTSEMRLYLCIVADGVGGRPGGEQASGTALRAIAQYATHTMR
                     LTSRNDEAHESRFLEELERAVAESHAAVLVDPESSATTLTMAVFRWPRTYLVHVGDSR
                     CYRLRDGRLELLTKDQTMAQALVDAGVMPASRAGETKWAHVLSSALGAEQPRPVITTL
                     NSQWDDAMLLCTDGLTKHVTDEEIAEQLATVKSSEAICHNLIDRALSRGGSDNVTVVA
                     GRLRERAPA"
     gene            2628..3731
                     /locus_tag="VFB89_11525"
     CDS             2628..3731
                     /locus_tag="VFB89_11525"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZM27981.1"
                     /translation="MPVAAPQAFVERQLYETLVRFDCTGRVRPALAQTWHPEDGGKVW
                     VVTLRPDAKYWDQIALTPQAIATTWAADSAAGPLVQAAGILAVAALGDHEVKLTLAAP
                     RDSLPRALADRGLALTRRAAGREPVGTGRYRPASGNNPSTLVPVDTGPHPVVRVLATR
                     DLRDALDAGADLVVTDDPGTLDYASARAELSSVPLTWNRTYVLVVPKRLGFSPDSAWS
                     ARLRETLARDAVRVDARAAEPPFPWGSCAGPVPAGAPQAEGARLAYPADDRTARDLAA
                     RIVAIGAAPARSIIGLDSLALRQSIRAAREPAYVLSVPKMPLIPCEGPILWPADVTIV
                     PLVETRPHAILRRGAPRVSVDWDGGLRLVPEQP"
     gene            3728..5434
                     /locus_tag="VFB89_11530"
     CDS             3728..5434
                     /locus_tag="VFB89_11530"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZM27982.1"
                     /translation="MSFRVRLLLAFVGVLLIPLGVLAFGVRREMDRRLTAESDRRVHA
                     LADVVQEDLRSRSGEVAARLAALRDELAADNRFRLAAVQADPAARGWLLDYAGGAMRV
                     AGLALLQVQDSSGRILSSGHFRNEFDRLDPELPALLAQPRDTLAVVRARTAEGDFLAL
                     ARVDSFRVGGRHFTIVGGAALDPQRIGQVDSAGGLAVSLALPGTAALPDQSGVVAVLG
                     LPFLDLTREGDVKLDTASVIVTQSLSQLEALRRNVNAWFLAALGLTTAAALLAAAWMA
                     RRVSQPLAELARKTGKIDLDQLDEDFATDRDDEIGALSRLLGDMTARLRQSAGRLREA
                     ERRATLGDVARQVNHDIKNGLAPIRHVLRHLTQVAADEPASLPQVFQERRGTLESSVT
                     YLDTLAGNYARLTPVMQAGPCSLNDVVREVARLSEGRASLLLALASDLPLVRGDELAI
                     RRVLENVVGNAADSLDGKTGAAVTITTERSGDPATPSVRVIVADQGRGMTQAELDHAF
                     DDFYTTKERGTGLGLTIVRRLIQDLGGRLRIETEPTIGTRVIIELPAIQAAPGNTSGD
                     RA"
     gene            5431..6792
                     /locus_tag="VFB89_11535"
     CDS             5431..6792
                     /locus_tag="VFB89_11535"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZM27983.1"
                     /translation="MTTVLVVDDIAALAEQYAYDLKRLANYETLVAIGGGQALEAIEQ
                     EALDCVVLDLEMPGMDGFEVLRELEHRGADVPVIVYTGTGNFDRCIQAVRAGAAGFID
                     KNEPMERVVRDIEAVLTRRRLEAEVRSLRRRLDAESSLLGDSPPVRKLRESIAKLAPI
                     PSPVLILGESGSGKELVARDLHRLGGNPKAPFVALNSAALPESLVESELFGHERGAFT
                     GAAAARRGAFEAAEKGTLFLDEIGELPLAAQAKLLRVLEERRVTRLGSNRLLAVDARV
                     VAATNRDLDAEVAAGRFRQDLLFRLNVHVIQVPPLRERRSDIPALTDAFLAGICERFG
                     VRRKRVAGAVLDGLMAYDWSRNNVRELRNAVERMVIAADGDVIGLEHLPAEIAETMPR
                     PGGRDGTSYQELRTEAERQIVTAALERNSWQVTRTAEDLGLADHASLLKIMRRLGVQR
                     PTR"
     gene            7005..8027
                     /locus_tag="VFB89_11540"
     CDS             7005..8027
                     /locus_tag="VFB89_11540"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM27984.1"
                     /translation="MSFSNPVVRLTLVTVSPLMALVVGCSGGEPTNAPAVATKVGSET
                     SATTTTSASTPTTTPTPVSYESAEAAYSAQRYPEAAELFSRYTEQKPENPWGFYMLGL
                     ASWKAGNLTQAEVAFDQALKLDPKHQKSWLNSARVLLEQGRADEASERAQSAIEHDSS
                     SGEAWRVLARAQVELGNVNEAIEANRRAIALDEHDVWAMNNLGLIYIQQGQYTEALPP
                     LARATQLKPTAPVFQNNLGQALERSGNPVAAQTAYEGALAADSTYAKASLGLERVANR
                     PADSTVTEVDVASLAREFQLSIEQWRDEFVQVTSEPEAQSDTLTTSSMARDSAQEMVT
                     QPDSTH"
     gene            complement(8203..8964)
                     /locus_tag="VFB89_11545"
     CDS             complement(8203..8964)
                     /locus_tag="VFB89_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27985.1"
                     /translation="MSPAIPWFITLIVIATNIAIAVAVWTIFARSRARGGAPRESGRG
                     TVNGLAFLLGVWPAAGIFIAPKLSLPVSQGGVAIPPVILLAGFGVAVVLVWLSVSSDL
                     RRTLAAAPLPAVIGVQVYRAIGAVFVFLLGLGQLPPHFALPAGWGDIVVGITAPVVAL
                     ALLRGGSGSRLLALSWNLFGLLDLVVAVGMGSGLLVPLLAPGQPSAPAAAMQQFPLVV
                     IPAFAVPVSILLHILALSGLRRRVQLRSGIVAPAR"
     gene            complement(9087..>10271)
                     /locus_tag="VFB89_11550"
     CDS             complement(9087..>10271)
                     /locus_tag="VFB89_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27986.1"
                     /translation="QEGSQPCRTTEVRLASGGQSPVVLFSTGSGSIERDVDVSTAAGQ
                     PALLQITGLGQSGITADARRTIYVEPSAALHELATVPGRVLDVLSGRVLWLDSTATVR
                     AVRIRSVAAGTDVTALTESGGGFLGGALTSTGAVFAFTAPGQNGRVHAFRNGSLTTVG
                     GHGSASLQVTGDFAVWNEGETLKRYDASTGALTTVAANAGNTSNSVGPNGDVVYWTNG
                     YQIVRVRGGTATPITSGPSVSVVPVTDGTNVVFRQAPPGFQGPWQTILFDGSNFIPLA
                     ASSPNDPSRELCYAVNAGWTGYCKLGSAQETQTWTRSPAGEERQASFFASGSLFEALS
                     PAGQVIFVASSAQAAPRRFLAVPPYTGNPSDIGGGSMGTPVFRGAAAYLLLGRSAFTI
                     AP"
BASE COUNT         1770 a         3461 c         3311 g         1729 t
ORIGIN      
        1 gcgaccaccg gtggccgcat gagcggggca gccctcgagc tgatcgggct ccgccacacc
       61 ttcttcccgg gaacgccgag cgagcagcgc gcgctcgagg gcgtgagcct cacggtcgat
      121 ccggggacct ttgtggtggt cattgggatc aacggatcgg gcaagtccac cctgctcaac
      181 ggcgtggccg ggagcgtcac gctggatgag ggaatcgtcc ggattggcgg cagggacgtc
      241 acccgctggc cggagcatcg ccgggccggg atgattggac gggtatttca gaacccgttc
      301 tcgggtaccg ctccccacct tactgtcgct gaaaacttgg cgattgcatc gcagcgaggc
      361 cgatcacgtc agctcttccg cctcggattg agcagcggcc gccgagccca actggccgag
      421 cagctcgcga gcatgaacct gggcctcgag catcgtctgg tcacgccgac cggagtcctc
      481 tccggcggtc agcgccaggc gctgacccta ctcatggcta ccatcgttcg gccggcggtc
      541 ctgctgctgg acgaacacac cgctgccctc gacccaagga gtgccgagca ggtgttgcgg
      601 ctcaccgaag agaccgtggc gcgtgaccgg ttgaccacgc tcatggtgac gcactcgatg
      661 caccaggccg ttcgcatcgg agaccggctg ttgctggtgc atcgcggtcg cattctggaa
      721 gacttttccg gccctcgaaa gcggcggctc cgaatggagg acctgctcga ccgcttcgac
      781 cagctccgga acgccgatat gctggacgaa tccgccgctg aaatgcttcg ccgcgcgtac
      841 gtgtagctag acaggaccgg acggatattg tatgcatggg gaaggagcgc tccgcccatg
      901 cctgccgttc ccgagtggcc tgcggtgcca ccccgccctg ctgtcgtctc cgccttttgg
      961 cgcgctgccc ggcttcattg tccggcctgc ggtggcggtc ccgtatttct gagttggatc
     1021 cgcatcgcac ccgcctgccc atcctgcggc ctcaacttca cccgcgggga acgcggctac
     1081 tggcttggcg cttatttcgt caatctgatg gcagtcgaag ccatctttgc cgtcatgctg
     1141 ggtatcgcat tgtggtggac ctggcccacc cccccttggg aaggcatcga gtgggcgctt
     1201 ggtgtcgcca tgctggtttc accgttcctg ctctatccct ggtcgcacac gattttcctc
     1261 gcgttcgatc tcttgttccg gccgccggcc tcggaagatt tcgaggcgcc gcacgagcct
     1321 gcacccgttt tccggcggcg gggtcgttag cagccccgag gatcccccgt cccgaggatc
     1381 ccccgtatgg aaggtcaggg cactaccgca cgcatcgacc atcaagggtt gcggtaacgg
     1441 cacgggcgcg catgttagtg gacccgaatg tccggcagga gcgccccgtg gccgtcgcaa
     1501 cagcgccatc tgttacaaac cggcccctcg caggcgacat cgacgttttc ggcctgacgc
     1561 atccgggcca cgtgcggcgg gtaaatcagg atcagttcct gatcgcaagc ctgcacaaga
     1621 ctatgcaggt gcacgcctcg agtgtgcccg ccacccagct cggcgacctc accagtgaaa
     1681 tgcgcctgta cctctgcatc gttgctgatg gcgtgggcgg gcggccgggc ggagaacagg
     1741 cctccggaac agcacttcga gccattgcgc agtacgccac gcacaccatg aggctcacct
     1801 ctcgtaacga cgaggcacac gagagccgat ttctggaaga gctggagcgc gcggtcgcgg
     1861 aaagccacgc agctgttctt gtcgatcccg aaagctccgc gaccaccctc acgatggccg
     1921 tgttccgctg gccccgcacg tacctggtgc atgtggggga cagccgctgt tatcgtttgc
     1981 gggacgggcg gctcgaactg ctcactaagg atcagaccat ggcgcaagcg ctggtcgatg
     2041 ccggcgtcat gccggcgtct cgcgccggcg aaaccaagtg ggcccacgtg ctctcgagcg
     2101 cgttgggagc cgagcagccc agacccgtca tcaccaccct caacagtcag tgggacgacg
     2161 ccatgctgct ctgtaccgac ggtctgacga agcacgtcac cgacgaggag atcgcggagc
     2221 agctcgcaac cgtcaagtcg tcggaggcga tttgccacaa cctgatcgat cgagcactct
     2281 cgcgaggtgg gtccgacaac gtcactgtcg tggccggtcg tcttcgggaa cgcgctcccg
     2341 cctaatccgc agcagttaca ttcacagcat cgatgtctcc atcctcgcgc cgagcggcgg
     2401 gcaccgccgc agcttggctg tggccgttcg cattggcgtg gaccgcatcc gcgtgcggca
     2461 cgccgtcccg atcccccgcc ccgggagcgc ccgagcccgc gccggctccg tcgcagccgc
     2521 ctcaaggacc ggctcgaacc cagccaggta aggccgagcc tccacctcag cttccaccgc
     2581 cggacgagtg cgtcctcaac cacgaaacca cacccagccg ggatactatg ccggtcgccg
     2641 cgccgcaggc gttcgtggag cgtcagctgt acgagacgct ggtgcgcttc gactgcaccg
     2701 gtcgcgttcg accggccctc gcacagacgt ggcatcccga agatggcggt aaggtgtggg
     2761 tcgtcacgct tcggccggat gcgaagtatt gggatcagat cgctctcact ccgcaggcaa
     2821 ttgcgacaac gtgggcggcc gactccgcgg caggtccgct ggtgcaggca gccgggattc
     2881 tggctgtcgc cgctctgggc gaccatgagg tcaagctgac gctcgcggct ccgcgtgatt
     2941 cgctgccgcg ggcattagcc gatcggggcc ttgccttgac gcgaagggca gcgggccgtg
     3001 agccggtcgg tactggccgc tatcggcctg cttccgggaa caacccctcc acgttggtgc
     3061 ctgtcgatac cggcccgcac ccagttgtgc gagtacttgc cactcgggat ctccgggatg
     3121 ccctcgacgc gggtgcggac ctggtcgtca cggatgatcc ggggactctc gactacgcgt
     3181 ccgctcgcgc cgagctttcc tcggtgccgc tcacctggaa tcgcacctac gttctcgtcg
     3241 tccccaagcg cttgggtttt tcgccggaca gcgcgtggag cgctcgactg cgggaaaccc
     3301 tggcccgcga cgccgtccgg gtcgatgccc gagccgccga gccgccattc ccatggggaa
     3361 gctgcgctgg ccccgtcccc gccggagctc cgcaagccga aggcgcccga ctggcgtacc
     3421 ccgcggacga ccgtaccgct cgtgacctgg cggccaggat cgtcgcgatc ggagcggctc
     3481 cggcacgctc catcatcggc ctcgactcgc ttgctctccg ccaatcgatt cgggccgccc
     3541 gagagccggc gtacgtgctc tcggttccga agatgccgct cattccctgc gaagggccga
     3601 tcctctggcc cgcggacgta accatcgtcc ccttggtcga gactcggcct cacgccatcc
     3661 tgcgccgggg agcgccccgt gtgagcgtcg attgggacgg aggccttcgt ctcgtcccgg
     3721 agcagccgtg agctttcgag tccggctgct gctcgccttc gtcggcgttc tgctgatccc
     3781 cctcggcgtg ctggcgttcg gtgtgcgccg cgaaatggat cgtcggctga ccgccgaatc
     3841 cgatcggcgg gtgcatgccc tggccgacgt ggttcaggaa gacctgcgga gccggagtgg
     3901 agaagtcgcg gcacgcctcg ccgccctccg ggacgagctg gccgcagaca atcgcttccg
     3961 acttgcggcg gtccaggccg accccgccgc tcgcggctgg ctgctggact acgcgggcgg
     4021 agccatgcgc gtggcgggtc tcgccttgct tcaggtgcag gacagcagtg gccggattct
     4081 gagctcaggg cattttcgca atgagttcga ccgcctcgac ccggagcttc cggcactgct
     4141 ggctcagccc cgtgacacgc tcgcggtggt gcgagcacgc acggccgagg gcgacttcct
     4201 cgctctcgcg cgggttgatt cgtttcgagt gggcggacgg catttcacca tcgtcggcgg
     4261 tgccgccctc gacccgcagc gcatcggcca ggtcgactcg gccggcggcc ttgccgtgag
     4321 cctggcgctt ccgggaacgg ccgctcttcc cgaccaatca ggtgtcgtag ccgtcctcgg
     4381 cctgcccttt ctcgatctca ctcgtgaggg cgatgtcaag ctcgacactg cgagcgtaat
     4441 cgtcacccag tcgctgagcc aattggaagc actccgccga aacgtgaatg cctggtttct
     4501 cgccgcgctg ggccttacca cggcggcggc actccttgcc gccgcctgga tggcgcgacg
     4561 ggtctcccaa cccctcgctg agttggctcg gaagaccggg aagatcgatc tcgatcagct
     4621 ggatgaggac tttgccaccg accgcgacga tgagatcggc gcgctctccc gtctgctcgg
     4681 cgacatgact gcacgactgc gacagagcgc gggacggctg cgtgaggcag agcgtcgtgc
     4741 tacgctgggc gacgtggcgc ggcaggtgaa ccacgatatc aagaacggcc tggcgcccat
     4801 tcgtcacgtc ctgcggcacc tgactcaagt tgccgccgac gagcccgcct cgcttccaca
     4861 ggtcttccag gagcggagag gcactctgga atcgagcgtg acgtacctgg acacgctcgc
     4921 cggcaactat gcccgtctca ctcccgtcat gcaagcaggg ccatgctcgc tcaacgacgt
     4981 ggtacgagaa gtcgctcgct tgtccgaggg ccgagcatcg ttgcttctgg cgctcgcatc
     5041 cgatctgccg ctggtgcgtg gcgacgagtt ggcgattcgg cgggttctcg agaacgtcgt
     5101 gggtaacgcc gccgacagcc tggacggaaa gacgggcgcc gcagtcacga tcacgacgga
     5161 gcgaagcggt gatccggcaa caccgtcggt gcgggtcatc gtggccgacc agggccgggg
     5221 gatgacgcag gctgagctag accacgcctt cgatgacttc tacaccacca aggaacgcgg
     5281 cacggggctc ggcctcacca ttgtgcgccg gttgatccag gacttgggcg gccgcctgcg
     5341 aatcgaaacc gagccgacca tcggtacccg agtgatcatc gagcttccgg caatacaggc
     5401 cgcgcccggc aacacctcag gagacagagc gtgacgacgg tgttagtggt ggacgacatc
     5461 gcggccctgg ccgagcagta cgcgtatgat ctcaagcggc tggcgaatta tgagactctc
     5521 gtcgcgatcg gcggagggca ggcgctcgag gcgatcgaac aggaggctct ggactgcgtc
     5581 gtcctcgacc ttgaaatgcc gggcatggac ggattcgagg tacttcgcga actggagcat
     5641 cgcggcgcag acgtgcccgt gatcgtgtat accggaactg gcaacttcga tcggtgtatc
     5701 caggccgtgc gcgcaggcgc ggccggcttc atcgacaaga acgagccgat ggagcgggta
     5761 gtccgcgaca tcgaggccgt ccttacgcgg cgcaggctcg aggccgaggt tcgctccctg
     5821 cggcgccgtt tggatgccga aagctccttg ctcggcgaca gtccaccggt tcgcaagctg
     5881 cgggaatcaa tcgcaaagct ggcgcccatt ccgagccccg tcttgatcct gggcgagagc
     5941 ggctccggaa aggagctggt cgcccgcgat ctccatcggc tgggggggaa ccccaaagcc
     6001 ccctttgtag cgctgaacag tgccgctctg ccggaatcgc tggtcgaaag cgagttgttc
     6061 ggacacgagc gtggggcatt caccggcgca gccgccgctc ggcgcggcgc attcgaagcc
     6121 gcggagaagg gcacactctt cctggacgag atcggggagc taccgctcgc agcccaagcg
     6181 aagttgctgc gtgtgctcga agagcggcga gtgaccaggc tgggcagcaa tcgcctgctt
     6241 gcagtcgatg cccgagtcgt ggccgcaacc aaccgcgatc tcgatgccga agtcgccgca
     6301 ggcagattcc ggcaagacct cctcttccgg ctcaacgttc acgtcattca ggtaccgccg
     6361 ctgcgcgagc gtcgctcgga tatccccgcg ctgacggatg cattcctggc cggcatttgc
     6421 gagcggttcg gcgtgcgaag gaagcgggtt gccggcgccg tgctcgacgg gctcatggcc
     6481 tacgactgga gtcgcaacaa cgttcgggag ctgcgcaacg cggttgagcg aatggtcatc
     6541 gccgcagacg gagacgttat cgggctcgag cacttgccgg cagagatcgc ggaaacgatg
     6601 cctcgacccg gtggtcggga tgggacctcc taccaggagc tccgaaccga ggccgaacgg
     6661 cagatcgtca cagccgcact cgagcgcaac tcctggcagg tcacccgcac ggcggaggac
     6721 ctcggccttg ccgaccacgc cagccttctc aagatcatgc gccggctggg agtgcaacgg
     6781 cccactcgat gagtcgtgtt cggccggaca cgctcacgac ccgagcgcgt gttcgctcga
     6841 acacgcccga ctttatcgcc tgacctgcct gcttcgctca acttgccgct ctgcagccat
     6901 ttagcagata tcagccgctc ctccattgca tggcccccgg gttgcctttc tggtggcgtc
     6961 ccgttcatcg ccacgcgcca ccggcgccgc accgaggagc agccatgtcg ttcagcaacc
     7021 ccgtcgtccg tctcacgctc gtcaccgttt cgccgctgat ggcgctcgtc gtgggctgca
     7081 gcggaggtga gcccaccaac gctccagccg tcgcgacgaa agtcgggtcg gaaacgagcg
     7141 cgaccaccac gacctccgcc agcactccga caaccacacc gacccccgtg agttacgaga
     7201 gcgcggaggc cgcctactcc gcccagcgct atcccgaagc ggccgagctg ttctcccgct
     7261 acaccgagca gaagcccgag aatccctggg gcttctacat gctcggcctg gcatcctgga
     7321 aggcagggaa cctgactcag gccgaggtcg cgttcgacca ggccctcaag ctggatccga
     7381 agcaccagaa gagctggctc aactcggcgc gtgtgctgct ggagcagggc cgtgcggatg
     7441 aggcctcgga gcgggctcag agcgcgatcg agcacgactc ctcgtcgggc gaagcctggc
     7501 gggtgctggc acgcgctcag gtcgagctgg gcaatgtcaa tgaagccatc gaggccaatc
     7561 gccgggccat tgcactcgac gagcacgatg tgtgggccat gaacaatctg ggcctgatct
     7621 acatccagca gggtcaatac accgaggcgc ttccgccgtt ggctcgtgcg acgcagctca
     7681 agcccactgc accggtgttc cagaacaatc ttgggcaggc cctggagcgc agtgggaatc
     7741 cggtggctgc gcagaccgcg tacgaagggg cgctcgccgc ggacagcacc tacgccaagg
     7801 cgtccctcgg ccttgaacgt gtggccaatc gccctgcgga ctcgaccgtg acggaggtgg
     7861 atgtcgcgag cctggcgcga gagttccagc tgtcgatcga gcagtggcgg gacgaattcg
     7921 tccaggtcac tagtgagccg gaggcgcaat ccgatacgct gacgaccagc agcatggccc
     7981 gcgattcggc ccaggagatg gttactcagc ccgattccac tcactgaccg acggtagctt
     8041 ctcccaaccc gcggtggttc gccggcctca gacggcgggc cgccgcggtt ctgctttgga
     8101 ttcccgcgct agccggtggc gaaggtggcc ggttagtcag ccaccctcga atccggcgga
     8161 cgtctggcca tgccctttcg gcgttgcacg agtgcctcat gcctaccggg ccggagcgac
     8221 gattccggac cgcagttgca cccgccgacg gagtccgctc agcgcaagga tgtggagcag
     8281 gatcgacaca ggaactgcaa acgcggggat caccaccagc gggaattgct gcatcgcggc
     8341 cgccggtgcc gaaggctgac ctggggcgag cagcggtacc agcaacccgc tccccattcc
     8401 gaccgccacg acgagatcga gaagcccgaa caggttccag ctcagggcaa gcaggcgact
     8461 ccccgaccct ccacgcagta gcgcaagcgc aaccaccggg gcggtgatcc ccacgacgat
     8521 gtccccccag ccggcgggca gcgcgaagtg cggagggagc tggcccagac cgagcaggaa
     8581 cacgaacacc gctccgatgg cgcggtacac ctgcacgccg atgacggccg gcagcggtgc
     8641 agcagcgagc gttcgccgga ggtcagacga taccgagagc cagaccagca ccaccgcgac
     8701 gccgaagccg gccaacagga tgaccggcgg gatcgcgaca ccgccctggc ttacgggcag
     8761 gctgagcttt ggtgcgatga agatgcccgc agcgggccag acaccaagca agaaggcgag
     8821 gccattcacg gtgcccctgc ccgactctcg cggcgcccca ccgcgcgctc gggagcgagc
     8881 aaaaatggtc cagacggcaa ctgcaattgc gatgttagtc gcgatcacta tcagggtgat
     8941 gaaccagggg attgcagggc tcataaggtg ctcctccaag cttgggactc ggccggcctg
     9001 cccgtgtcgg acactacggg tcgacccgtc ataaagatgt atatgcatct aattccccca
     9061 acgcgcaaga gccctgtcgg ggattgttac ggtgcaatag tgaatgctga acggccgagc
     9121 agcaggtagg cggcagcgcc tcgaaacacg ggcgtgccca tcgagccgcc gcctatgtca
     9181 gagggattcc cggtgtaggg tggaaccgcg aggaagcgcc ggggagcagc ttgagcgctg
     9241 gaggcaacga agatgacctg tccggccgga ctcagcgcct cgaacaagct tccgctcgcg
     9301 aagaaggaag cctgtcgctc ttcacccgcg ggggatcgcg tccaagtctg cgtctcctga
     9361 gctgatccca gcttgcagta gccggtccaa cccgcgttca ccgcgtaaca cagctctcgg
     9421 ctggggtcat ttggcgatga cgccgcgagt gggatgaaat tactgccgtc gaacagtatt
     9481 gtttgccagg gcccctggaa tccgggcggc gcttgccgaa agacgacgtt cgtcccatca
     9541 gtcacgggaa cgacgcttac cgacgggcca ctggtgatcg gcgtcgccgt acccccgcga
     9601 acccgcacga tctggtagcc attggtccag taaaccacat cgccgttcgg tccgacgctg
     9661 ttcgacgtgt tgccagcgtt agccgcaacc gttgtgagcg caccagtaga cgcatcgtat
     9721 cgcttgagcg tctcgccctc gttccacacg gcaaagtcgc cggtgacctg caacgaagcg
     9781 ctgccatgcc cgccgaccgt cgtcaagctc ccgttgcgga aggcatgcac tcgcccgttc
     9841 tggcctggcg cggtgaaggc aaaaaccgcc cccgtggatg taagcgcgcc gccgaggaac
     9901 ccgccgcccg attcggttag cgcggtaaca tcagtgccgg ccgccacact ccggatcctc
     9961 acagctcgca ctgtcgctgt ggaatcgagc cacaagactc ttcccgagag aacatcgagc
    10021 actcggcccg gaacggtggc gagctcatgc agcgcagcgg agggctcgac atagatcgtc
    10081 cggcgggcat ccgcagtaat gccgctctgt cccagccccg tgatctgcag cagcgccggc
    10141 tgccctgcgg cagtgcttac atcgacatcc cgttcaatcg agccagaacc ggtgctgaac
    10201 aggaccacag gtgattgacc tcccgacgcc agccggacct ccgtggtccg gcacggctgc
    10261 gagccttcct g
//
LOCUS       DASRJQ010000502         3185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3081841, whole genome shotgun sequence.
ACCESSION   DASRJQ010000502 DASRJQ010000000
VERSION     DASRJQ010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3081841"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /gene="asnB"
                     /locus_tag="VFB89_11555"
     CDS             complement(<1..905)
                     /gene="asnB"
                     /locus_tag="VFB89_11555"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HZM27987.1"
                     /translation="MCGIVAIYDSQGRVSPEALRRATAQLHHRGPDDRRHWIAAHGRV
                     GLGHTRLSIIDLVTGDQPIASEDERRHIVVNGEFYDFERIQCELERAGHRLRTRSDSE
                     IALHLYEDLGPHCLHQLRGEYAFVLWDEPQQRLFAARDRFGIKPLFFAWYQGALYLAS
                     EVKALFAAGVPARWNREGLYNSAIFGGQPTDTLYEGVSQLPPGCYLVAGPNGMQIHRY
                     WDFDYPLAKDWQPTRSDQDWAEEFRVVLENAVRLRLRADVPVGCYLSGGLDSCTVLGL
                     AARHSAEPIRAFTLAFDLPAYDEEA"
     gene            complement(1172..1528)
                     /locus_tag="VFB89_11560"
     CDS             complement(1172..1528)
                     /locus_tag="VFB89_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27988.1"
                     /translation="MRVFLALIAAGSLAACSHNNSNNEPQPGPETGVVSTDTGVTRAP
                     IDTMQTTRTPAGTIDTSRAGSDTSGRRNMPPASTSDTTFIGAQPGETHHADSALGGAD
                     TSSIRPDTTSLRHDSL"
     gene            complement(1698..2915)
                     /locus_tag="VFB89_11565"
     CDS             complement(1698..2915)
                     /locus_tag="VFB89_11565"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZM27989.1"
                     /translation="MKLEAHPDRTLIRAVGSSVRYIRVSLTAPPSTSRPKRWPVHLAL
                     VLDRSGSMGGRKIALARDAVGQTLRLLGPDDQFALVVYDNEVSLLSASVSATPEAKQR
                     ALERLARIDARGSTDLSGGWLQGTEQLLRTLSADAIGICFLLTDGLANHGITDRAQLM
                     RMAGDLRARGVCTSTFGVGNDFDEGLLRAMAEASSGNFHYIEAPEEIPELLTRELGEK
                     LEVVARDVALEVTLAPGVSAEPLTRLPWKQSGGQLRVQLGHLVSNQEVELVIKLLFPS
                     GEAGRQVATTLSVAHPEVANTSTEEIRWTWARQEENDHQSRNREVEWAVATLYAGRVR
                     EEAVERSREGDLEGARRVLHATARCIRKYAGDEPRLRTLVAQLEAEAEEYGARPFTTR
                     EIRKAIYADYVAA"
BASE COUNT          534 a         1002 c         1062 g          587 t
ORIGIN      
        1 atcgcctctt cgtcgtatgc ggggaggtcg aatgccaggg tgaaggcgcg gatcggctcg
       61 gcggaatggc gcgcggccag accaagtact gtgcacgagt cgaggccacc gctcaagtag
      121 cagcccaccg ggacatcggc gcggagccgg agccgcaccg cgttctccag taccacccga
      181 aactcctcgg cccagtcttg gtccgagcgg gtcggctgcc agtctttcgc gagcgggtaa
      241 tcgaagtccc aataacgatg gatctgcatt ccattgggac cggccaccag gtagcagcct
      301 ggcggcagct gcgacacgcc ttcgtagagc gtatcggtcg gctggcctcc gaagatcgct
      361 gagttgtaca acccttcgcg attccagcgg gcggggacgc cggctgcgaa cagagccttg
      421 acctcggagg ccaggtagag ggcaccctgg taccaggcga aaaagagcgg cttgatgccg
      481 aagcgatcgc gggcagcgaa cagccgctgc tgcggctcgt cccagagcac gaaggcgtac
      541 tcgccgcgca gctgatgcag gcagtgcgga ccgagatcct cgtagaggtg gagcgcgatc
      601 tcgctgtcgg agcgggtgcg gagccggtga ccagcgcgtt ccagctcgca ttggatccgc
      661 tcgaagtcgt agaactcacc gttgaccacg atgtgccggc gctcgtcctc gctcgcgatc
      721 ggctgatcgc cggtcaccag gtcgatgatg ctgagacggg tatggccgag tcccacccgg
      781 ccgtgcgccg cgatccagtg gcggcgatcg tcgggaccac ggtgatggag ctgcgcggtt
      841 gcgcgccgca gcgcttcagg tgaaacgcgt ccctgcgagt cgtagatcgc gacgatgccg
      901 cacatgccgt aactccgaaa ggcttatgtc gggtaagtac gttaacagtt acggtgccgg
      961 actcgcagtc gccaggggaa gaccgtccgg gtcaggtcct ggcgcggtat agtgtcagtc
     1021 ggcgtcctac tggacgggag cagtgtaggg tgtgatgcaa gcggccagtt gcgcggaccg
     1081 tgatcgctgt agtgaggaca aagacaaggg gccagcatcc accggtgccg gcccctttcg
     1141 tcgatcatcg gctgacccga tcagtgtgtg actatagtga gtcgtgacgg agcgacgtgg
     1201 tgtcggggcg gatggacgaa gtgtcggccc cacccaaagc gctgtcggca tgatgtgtct
     1261 cgcccggctg cgcgccgatg aaggtggtgt cgctcgtgga cgcaggtggc atgttgcgac
     1321 ggcccgacgt atccgagccg gcgcggctcg tatcgatggt accggctggc gtccgggtcg
     1381 tctgcatcgt atcgatgggt gcccgcgtca ccccggtgtc ggttgagacc actcccgttt
     1441 ccgggcccgg ctgaggctcg ttgttgctgt tgttatgcga gcacgcggcc agcgagccgg
     1501 ccgcaatcag ggcgagaaag acccgcatga ttaccccctc tgcaaggtga ctacggccgc
     1561 gccgcatcat tgtgaagcgg ccgatcgtct accctaccct gccgctgggg ctcttgcccg
     1621 caatcccgat caccaaactc tcggacaccc gtgacccgag tcacagaagc gatccggccg
     1681 tgggactgcc gggcggctca tgcagccaca tagtcggcgt agatcgcctt tctgatttcg
     1741 cgggtggtga acggccgcgc tccgtactct tcggcctccg cttccagctg tgccaccaga
     1801 gttcggagcc gcggctcgtc gcccgcgtac ttccgtatgc accgagccgt cgcatgcagg
     1861 actcgtcgcg caccctcgag gtctccttcc cggcttcgct cgactgcctc ctctcgtact
     1921 cggcctgcgt acagcgtcgc aaccgcccat tcgacctcgc ggttgcgaga ttgatggtcg
     1981 ttctcctcct ggcgggccca ggtccaccgg atctcttctg tggatgtgtt cgcgacttca
     2041 gggtgggcaa ctgaaagggt tgtcgctacc tgcctgcccg cctctcccga tgggaacagg
     2101 agcttgatca ccagctcgac ttcctggttg gagaccaggt gccccagctg gacccgtaac
     2161 tggcctccgc tctgcttcca cgggagtcga gtgagcggct cggcggacac tccaggtgcg
     2221 agcgtgactt cgagcgccac gtcgcgtgcc actacctcca gcttctcgcc cagctcacgc
     2281 gtgagcagct cggggatctc ctccggcgcc tcgatgtaat gaaagttgcc tgacgaggcc
     2341 tcggccatcg cgcggagcaa gccttcgtcg aagtcgttcc cgacgccgaa cgtggaggtg
     2401 cagacccctc gggcccgaag gtcccctgcc atgcgcatca gttgggcccg gtcggtgatg
     2461 ccgtggttgg cgagtccgtc agtgagcagg aagcagatgc cgatggcatc ggccgatagt
     2521 gtcctcagca gttgctcggt accctggagc cagcctccgg aaagatcggt cgagccgcgt
     2581 gcgtcgatgc gggccagccg ctcgagtgcg cgctgtttcg cctccggggt ggccgagacc
     2641 gaggccgaga gcagactgac ctcgttgtcg tagaccacga gggcaaactg atcatcggga
     2701 ccaagcagtc gaagcgtctg cccgacggca tcccgtgcga gcgcgatctt ccgccctccc
     2761 atcgagccgg agcggtcgag gaccagagcg aggtggacgg gccagcgctt gggccggctc
     2821 gtgctcggcg gtgccgtcag tgaaacgcgg atgtaacgaa cgctgctgcc gactgcgcgg
     2881 atcagagtgc gatccgggtg tgcctcgagc ttcaccgtag tctccaaggg ctcgggccgg
     2941 cgatcttgag ctggacgatt ctaccggtac ccatgcgtgc ttagacggcc cggaatcgtg
     3001 aatttcacag ggtctcccta cgctcttgtc cgttgccggc ctacacacaa cgcgaaaaga
     3061 tccgatcgat agccggagcg cctgccgatg acggcggacg ttgcggtttc ttaacctgtg
     3121 agtcggctca gcgctggtgg aagtcaggtt tcgagttggc tcattcggac aggagagacg
     3181 gagat
//
LOCUS       DASRJQ010000503         3621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1228405, whole genome shotgun sequence.
ACCESSION   DASRJQ010000503 DASRJQ010000000
VERSION     DASRJQ010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228405"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VFB89_11570"
     CDS             <1..528
                     /locus_tag="VFB89_11570"
                     /inference="COORDINATES: protein motif:HMM:NF039739.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6361 family protein"
                     /protein_id="HZM27990.1"
                     /translation="RIREQLDHAQNISELMWGAALLYNLMLARLSERSELVQSYEQRL
                     AGWSTMIEARHDALRAWDRRRFWDILLSAGARPTPLTRAFIDTWIAHTLGNPRGMAAN
                     ASVQRLIRDRECALKGGQARLDNRRALENWGGAAGSAQLSFRWPVAQRMVEDILSGLA
                     ARSAPVPVPAVPLHA"
     gene            521..2461
                     /locus_tag="VFB89_11575"
     CDS             521..2461
                     /locus_tag="VFB89_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HZM27991.1"
                     /translation="MLEPTDRQLLLEALRPPQGYSLDYAVGTTFSLDLLALLTTPLAF
                     ALFDWQDHEGRPIADPLALLQAVRRYANRMAVFCQAGQIAIPRRDQLLYGYLETAVFE
                     VNPPRDSGVFHPKVWLLRFTLDADEAAEVRYRLLCLSRNLTFDRSWDTVLSLEGTVLP
                     RRNALAMNHPLGDFVAALPGLARHPPPEQVEERITTIAAEVRRARFEAPDGLTLERFW
                     PIGVRPAYRRWPFPPLPRRVLVVSPFLSDGFMGRFAAPGGNNVVISRTEALDELASTV
                     HGLFAQQLVLNDAANEEPREASAAPDLADMAPPVEDASPTDLSSETPLDASLVGLHAK
                     LFAVEEGWDVRLWTGSANATSAAFGDNVEFLVELKGRRAQLGIDALLARPQGRTSFAD
                     LLQTYSPPDTPVVADAVRQRLELMVDSARRQLGRAELAVDVATADEAERQEYILAVSS
                     KDSIALPAGVQLRCWPVTLREEAACLVPSIGQPTAAVFARFGPVSFEALTSFFAFSVT
                     AVEGNREVTTRFVLNIPVSGAPPDRRERMLLSLLRNRQQVLRFLLFLLAEGSADMAGA
                     LAAARKIGDGSVDGSDGAFGSQFPLLELLLRALHRQPVKLDQVARLIEELSSTEEGRQ
                     LLPDGWERVWQPIWSAREQAMP"
     gene            2458..>3621
                     /locus_tag="VFB89_11580"
     CDS             2458..>3621
                     /locus_tag="VFB89_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27992.1"
                     /translation="MTDNTRGGEARPDVNAILAGLKDFQRDTVEYVFRRLYLDQDTTH
                     RFLVADEVGLGKTLIARGVIARAVDHLWDLVPRIDIIYICSNGDIARQNVNRLRIGDQ
                     ESFALASRITMLPAQVHDLRGKKVNFISFTPGTSFNLRSSGGLVEERALLYWLLKRAW
                     GLSNAMAPLNVLQAGAHHERFRSWVRWYDQQGDIDEEISIAFKQALERRIAHEGQESN
                     LRTRFDDLCRRFPRGNTRASRPDEHARYRLIGELRSLLATTCLAALEPDLIILDEFQR
                     FKSLLHGTDAASELARGLFEYSDERSRARVLLLSATPYKMYTLADEAASDDHFADFAN
                     TVSFLDPHADPTAFLQLLRDLRGGLYRLADEGGERLPALKAGLETRLRRIMVRT"
BASE COUNT          590 a         1209 c         1173 g          649 t
ORIGIN      
        1 cgcattcgag agcaactgga ccatgcccaa aacatctccg agctgatgtg gggtgccgcg
       61 ctgctctaca acctgatgct ggcccgattg tcggagcggt cagagcttgt ccagagctac
      121 gagcagcggc tagccggctg gtcgaccatg atcgaagctc ggcacgatgc tttacgcgcc
      181 tgggatcgcc gccggttttg ggacatcctg ctctcggcgg gtgcacggcc gacgccactg
      241 acccgtgcct tcatcgatac ctggattgcg cacaccctgg ggaaccctcg cgggatggcg
      301 gcgaacgcgt cggtgcagcg cttgatccgc gaccgagagt gcgcgctcaa gggcggtcag
      361 gcccggctgg acaaccgccg cgccctggag aactggggcg gcgcggccgg ctctgcccag
      421 ctcagcttcc gctggcctgt ggcgcagcgc atggtggaag acatcctgag tggactcgct
      481 gcgcgatcag ccccggtgcc agtcccggcg gtgccgctcc atgcttgagc ccaccgatcg
      541 ccaactgctc ctcgaagcac ttcggccgcc tcagggatac tcgctcgact acgcagtggg
      601 gacgacgttc tcgctcgatc tgctcgcgct gctgaccacg ccgctcgcat ttgccctctt
      661 cgattggcaa gatcacgaag ggcgtccaat cgcggatccc ctcgccttgc tgcaagccgt
      721 gcggcgctat gccaatcgca tggccgtctt ctgccaggcg gggcagatcg ccattccccg
      781 gcgcgatcag ctgctctacg gctacctgga aaccgcggtc ttcgaggtca acccaccgag
      841 ggacagcggc gtctttcacc ccaaggtctg gctgctccgc ttcactctgg atgcggacga
      901 agcggccgag gtgcgctacc ggctcttgtg tctctcgcga aatctcacct tcgatcgctc
      961 gtgggacact gtgctctcgc tcgaaggcac ggtgctccca cgccgcaacg cgttagcgat
     1021 gaatcatcca ctgggcgact tcgtggccgc gctcccgggg ctggcccggc atccccctcc
     1081 cgagcaggtc gaggagcgca tcacgacgat cgccgctgaa gtgcgtcgtg cgcggttcga
     1141 ggcgccggat ggcctcacgc tggagagatt ctggccgatc ggagtgcgcc cggcctatcg
     1201 acgctggccc ttcccaccac ttccgagacg tgtgctggtg gtgtcgccgt ttctgtcgga
     1261 tggattcatg ggtcgattcg cggcgccggg cggcaacaac gttgtcatct cgcggacgga
     1321 ggcgctggac gagcttgcca gcacggtgca tgggctgttt gcgcaacagc tcgtgctcaa
     1381 cgacgcggcc aatgaggagc cacgggaggc atccgcagcg cctgacctgg cagacatggc
     1441 gccgccagtg gaggatgcct cgcccaccga tctctcgtcc gaaacgcccc tcgacgcgtc
     1501 gctggtgggg cttcacgcca agctgttcgc ggtcgaagag ggctgggatg tacgtctctg
     1561 gaccggatcg gccaatgcca catcggccgc gttcggcgac aacgtcgagt ttctggtcga
     1621 gctcaagggg agacgtgcgc agctcggcat cgatgcgctg ctggcgcgac cgcaaggacg
     1681 gaccagcttc gcagacttgc tgcagacgta tagccccccg gacactccgg tggtcgcgga
     1741 tgccgtccgg cagcggttag agctcatggt tgatagcgca cgccgccagc tcggtcgagc
     1801 tgagctagcg gttgacgtgg cgactgccga cgaggcggag cgtcaggagt acatcctggc
     1861 agtgtcctcg aaggattcga tcgcgcttcc cgccggtgtt cagctccggt gctggccagt
     1921 cacgctgcgc gaagaagccg cgtgcctcgt gccttcgatc ggccagccga cagcggcggt
     1981 cttcgcccgg tttggtccgg tctccttcga agcactcacg tcgttcttcg ccttcagcgt
     2041 gactgccgtt gaaggaaacc gggaggtgac cacgcggttc gtgctcaaca tccctgtctc
     2101 cggcgcaccg ccggaccggc gtgagcgcat gctgctctcg ctcctgcgta accgccagca
     2161 ggtgctgcgc ttcctcctgt tcctcctggc cgaggggagc gccgacatgg ccggtgcgct
     2221 ggccgccgcc cgaaagatcg gcgacgggtc cgtggatggg tccgatggag cgttcgggag
     2281 ccagttcccg ctcctggagc tgctgctacg ggcgctgcac cgtcaaccgg tcaagctgga
     2341 tcaggtggcg cgcttgatcg aagagctcag cagcactgag gaaggccgac agctcctgcc
     2401 ggatgggtgg gaacgtgtct ggcagccgat ctggtctgct cgtgaacagg ccatgccatg
     2461 accgacaaca cacgaggcgg cgaggcgcgg ccggacgtga atgccatcct ggcgggactc
     2521 aaagacttcc agcgcgacac ggttgagtac gtcttccgac ggctgtatct ggatcaggac
     2581 accacgcatc gcttcctcgt cgccgacgag gtcgggctgg gcaagacact catcgcccgc
     2641 ggcgtgatag ccagggcggt tgaccatctc tgggacctgg tcccacggat cgacatcatc
     2701 tacatctgct ccaacggtga catcgcgcgg cagaacgtca accgtctgcg catcggagat
     2761 caggagagct tcgccctggc gtcgcgcatc acgatgctcc ctgcccaggt acacgacctg
     2821 cgcgggaaga aggtgaactt catctccttc acgccgggca cgtcgttcaa cctgcgctcc
     2881 agcggtggcc tcgtcgaaga gcgcgcgctc ctctactggc tgctcaagcg cgcgtggggg
     2941 ctctcgaacg ccatggcccc gctcaacgtg ctccaggccg gagcacacca cgagaggttt
     3001 cgctcgtggg tgaggtggta cgaccagcaa ggtgacatcg acgaggagat ctccatcgcc
     3061 ttcaagcagg cgcttgagcg gcgcatcgcg cacgaaggcc aggagagcaa cctgcgcacg
     3121 cgctttgatg acctgtgtcg gcgcttccca cgcgggaaca cccgcgcctc gcgtcccgac
     3181 gaacacgccc gctacaggct catcggcgaa ctgcggagcc tgttggccac cacctgcctt
     3241 gccgccctcg aaccggacct gatcatcctg gacgagtttc aacgcttcaa gagtctcctg
     3301 cacggcaccg atgccgcgag cgagcttgcg cgcggcctgt tcgagtactc ggacgagcgc
     3361 tccagggcgc gggtcctgct gctctccgcc acgccgtaca agatgtacac ccttgcggat
     3421 gaggcggcat cggacgacca ctttgctgac ttcgcgaaca ccgtgagctt cctggacccg
     3481 cacgcagatc ccacggcgtt tctacagctc ctgcgtgacc tccgcggtgg gctgtaccgc
     3541 ctggcggatg agggcgggga gcgtctgccg gcactcaaag ctgggttgga gacgcggctt
     3601 cggcgaatca tggtccggac c
//
LOCUS       DASRJQ010000504         3988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2597809, whole genome shotgun sequence.
ACCESSION   DASRJQ010000504 DASRJQ010000000
VERSION     DASRJQ010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2597809"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="VFB89_11585"
     CDS             <1..1051
                     /locus_tag="VFB89_11585"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZM27993.1"
                     /translation="INLVPGLAITALVPILVKIDKPDLSLLKQADYLGIALMAVGLGM
                     LEYVLEEGTRWNWFDDATIRTCAWTAGIALALFVARSLTYRSPVLDLRALTNRNFTLG
                     CVLSFITGIGIFTTIYLTPLFLGYLRGFSAWQTGTAIFSTGAASLIGTPVYIALARRF
                     DGRWLMMFGLACFGVAMWSYSFITHDWGAGQFLVPQLLRGFPQVFAVAPAVILGLGSL
                     PPERLKYASGLFNMMRNLGGAVGIAVSAAIINDRTNLHFLHIASHLTPANGTMHRFVH
                     AVEGRYGALPGSEQGGHVAALEQLWHLAYREASTLAFADAFHTIMLAFVIATLLVPLM
                     RRVAPAAPAAATSAH"
     gene            complement(1466..2029)
                     /locus_tag="VFB89_11590"
     CDS             complement(1466..2029)
                     /locus_tag="VFB89_11590"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HZM27994.1"
                     /translation="MRDQLIASGLETLHREGFNGSAVQDITDAAGVPKGSFYNYFESK
                     EALALEVIDRYGEEVRALAGVLSDESLSPLERLRRYFALVATKFAARSYERGCLIGNL
                     SNELADQSRLIRDRLSSNFAAWSRPIETCIRDGQKAGEISRDMDARVLADFLLNSLEG
                     AMLRTKVEKDSSALDYFMTLVFSRVLT"
     gene            2251..2385
                     /locus_tag="VFB89_11595"
     CDS             2251..2385
                     /locus_tag="VFB89_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27995.1"
                     /translation="MSALYSGLPAATSVQQSIILAQLELLRRLDPARVEAAKAVLLRD
                     "
     gene            2415..3173
                     /locus_tag="VFB89_11600"
     CDS             2415..3173
                     /locus_tag="VFB89_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27996.1"
                     /translation="MYSTTQLLGATVLIVASTAQGQTPERVLMSHASASYSTAGTLSD
                     TETPRGDVAAALRVTGERALLIELDNRPRPAEPATLTDPPIDGSRALTGKGPSALPVA
                     PRAVERRSSFGAEIRGAVATSASGDAEFGAVQNPDRSPGAFVVSLGVRSDQGAILFTR
                     TNGTPLGVGRYRISEAAEGADEILALILTGSASRPTGVFRGQSGWLIVTEASERLLAG
                     WFQIDGVGFRAAEPERDDRRVSATGSFSAAAASS"
     gene            3385..>3988
                     /locus_tag="VFB89_11605"
     CDS             3385..>3988
                     /locus_tag="VFB89_11605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017397564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZM27997.1"
                     /translation="MGTSNIADKVVVITGASSGIGESTARLLAGKGAKVVLGARRKDR
                     LDAVVNDITAKGGSALGFKTDVTQRGDVEALVHGAIDKHGRIDVIVNNAGIMPIAPMA
                     ALKVDEWDRTIDLNIKGLLYGVAAVLPIMQKQKQGQIINMASILGFKVFAPGGVVYSA
                     TKFAVRALTEGLRMELKADKIRCTVISPGAVATELTESTSE"
BASE COUNT          711 a         1302 c         1220 g          755 t
ORIGIN      
        1 tatcaacctg gtaccggggc tcgccatcac ggcgctggta ccgatcctgg tcaagatcga
       61 caagccggat ttgtccctgc tcaagcaggc cgattacctc ggcatcgcgc tcatggcggt
      121 tggactgggg atgctggagt acgtcctcga ggaaggtacc cgctggaact ggtttgacga
      181 cgcgaccatc cgcacctgcg cctggaccgc cggcattgcc ctcgccctgt ttgttgcccg
      241 gagtctcacc taccggagcc cggtgctcga cctccgcgcg ctcaccaacc gcaacttcac
      301 gctcggttgc gtgctctcct tcatcacggg catcgggatc ttcaccacca tctatctgac
      361 cccgctgttc ctgggctacc tgcgcggatt cagcgcctgg cagaccggca cggcaatctt
      421 ctcgaccggc gccgcctcgc tcatcggcac gccagtctac atcgccctcg cgcgccgctt
      481 cgacgggcgc tggctgatga tgtttgggct ggcctgcttc ggggttgcca tgtggagcta
      541 cagcttcatc acccacgact ggggcgcggg gcagttcctt gtgccgcagc tgctgcgcgg
      601 gttcccacaa gtgttcgcgg tggcgcccgc tgtcattctc ggcctgggga gcctgccgcc
      661 ggagcggctc aagtatgcca gcggcttgtt caacatgatg cgcaacctgg gcggcgccgt
      721 cgggatcgcg gtcagcgcgg cgatcatcaa cgaccggacg aatcttcatt tcctccatat
      781 cgcttctcat ctgacgccgg caaacgggac gatgcaccgg tttgttcacg cggtggaagg
      841 acgctatggc gcgctcccgg gcagcgagca gggcggccac gtcgccgcac tcgagcaact
      901 ctggcaccta gcttatcgcg aggcctccac cctcgccttt gccgatgcgt tccacacgat
      961 catgctcgct tttgtgatcg caaccctgct ggtgcccctc atgcggcgag tggcacctgc
     1021 agcaccggcc gctgctactt cggcgcactg agcaacctgc ttcgcactcg aacttggagg
     1081 acacaatgtc cgcgctctgc tcttcgcccc tctgctcccg caccatgtac tcggcgcacc
     1141 aggcctgcca gtcctcgtcc cgctggcccg tcccaggaga aacgccccac cacaggagaa
     1201 cggcccacca cgccaacacc gctatggcgg tcgtgctgcg cggtttcgtc cgtgtcaatt
     1261 gtctggcaaa gtccgacatt tgagcgagga gacggcggca aagtccagca ctgaatccgg
     1321 caaaccccag cagcgggtgc cacgacctcg cgtgaatgct cgcaagttgc tggaattcgg
     1381 tggattagat cggcccctct agatcagcag agtcctcgtt ccgcccagcg gtctgatggt
     1441 gatcggctcg gtgaagaacg cgatcctagg taagaacgcg cgaaaagacc aaggtcatga
     1501 agtagtcgag agcagagctg tccttctcca ccttcgtgcg cagcatcgcc ccttccaatg
     1561 agttgagcag gaaatcggca agtacgcggg catccatatc ccgcgagatc tcacccgcct
     1621 tctgcccatc ccggatgcag gtctcgatcg gccgcgacca ggccgcgaag ttcgagctga
     1681 gtcgatcccg aatgagacgg ctctgatcgg cgagctcgtt gctgagattg ccgatcaggc
     1741 agcctcgctc gtagctccga gcggcaaatt tggtcgccac caacgcgaaa taccgccgca
     1801 gccgctccag cggcgagagc gactcgtcgc tcagcacgcc ggccagcgcc ctcacctcct
     1861 cgccgtagcg gtcgatgacc tcgagggcga gcgcttcctt gctctcgaag tagttgtaga
     1921 acgaaccctt gggcacgccg gccgcatcgg tgatgtcttg caccgcggag ccattgaacc
     1981 cctcccggtg gagcgtctcc aagccgctgg cgattagttg gtcacgcatg ctgggtctag
     2041 gcatggaacg aatatggccg gtcgtcttca ctgcgtcaag tcctgtcatt gagccgtgca
     2101 acttatgccg ttctgacgtc ttgacaattt aagatgaccg gtcatattaa atgctcacgg
     2161 actgcggcac cctttccagg tggctggtag aagtccgcca gaagaattcg cgcgaggtcg
     2221 attctcgcgc tcgactcgga ggataggaca atgtccgcgc tctattccgg ccttccggct
     2281 gccacctcgg tccaacaatc gatcatcctc gcgcaattgg aattgcttcg ccgcctcgat
     2341 cccgcccggg tagaggccgc gaaagcggtg ctgctgcgcg actgacctcc cactattcaa
     2401 ccggagaggt ttccgtgtac tccaccacgc agctcctggg tgcgaccgtc ctcatcgtcg
     2461 cgtccaccgc tcaagggcag acgcccgagc gtgtcctcat gagccacgcc tcagccagct
     2521 acagcaccgc cggaacgctc agcgatacgg aaaccccgcg gggcgacgtg gccgctgccc
     2581 tccgcgttac tggtgagcgc gccctgctga ttgagctcga caatcgcccc cgccccgcag
     2641 agcccgccac cctgacggac ccaccgatcg atgggtcacg ggccctcacg ggtaaagggc
     2701 catcggcttt gccagtcgct cccagggccg tggagaggcg gtcttccttc ggcgccgaga
     2761 ttcgcggggc tgtcgccacc agcgcatccg gcgacgcaga gttcggggcg gtccagaatc
     2821 ccgatcgctc ccccggtgcc ttcgtcgtgt cgcttggggt gcgcagcgac cagggtgcca
     2881 tcctgttcac ccgcacgaat ggaactcctc tcggcgtcgg ccgctatcga atctcagagg
     2941 ccgccgaggg agcggacgag atcctggccc tgatcctgac cggctcggcc agccgtccga
     3001 ccggcgtgtt ccggggccag tcgggctggc tgattgtgac tgaagcgtcg gaacgcctcc
     3061 tggccggctg gtttcagatc gacggagtag ggttccgggc agccgagccg gaaagggacg
     3121 accgacgcgt gagtgccacc ggatcgttct cggccgctgc agcgagctcg tgattccgag
     3181 tgcgtgaggg cgcggcgggc cgatccgatc gtctgcctct gtgggccgac cggagcgcca
     3241 atctgtcgac tcacaccgac gtagcggctt cttgcactca ttggttcagg aagtccgcga
     3301 tcgcgggggt ggtcatcgag tggagcgcaa accgccgtca gaaacattcg cattcttaat
     3361 tgtcttgata cggggaaaca gacgatgggg acgtcaaaca ttgcagacaa agtcgtagta
     3421 atcaccggag ccagcagcgg cattggcgaa agcacggcgc gcctgctcgc ggggaagggc
     3481 gccaaagtcg tgctcggagc caggcggaag gatcggctcg acgccgtcgt caacgacatt
     3541 accgccaagg gcggcagcgc tctcggtttc aagaccgatg tgacgcagcg cggtgatgtg
     3601 gaggctttag ttcacggcgc gatcgacaag cacggccgga tcgacgtgat cgtgaacaat
     3661 gctgggatca tgccgattgc gcctatggcg gcgctcaagg tcgatgaatg ggaccggacg
     3721 attgatctga acatcaaggg cctcttgtac ggggtggccg cggtgctgcc gatcatgcaa
     3781 aagcaaaagc agggtcagat catcaacatg gcttcgatct tgggcttcaa agtcttcgcg
     3841 ccgggcggcg ttgtttacag cgcgacgaaa ttcgccgtgc gggcgttgac ggagggtctg
     3901 cgcatggaac tcaaggccga caagattcgc tgcaccgtga tctctcctgg cgccgttgcc
     3961 accgagctga cggaaagcac ttcggaag
//
LOCUS       DASRJQ010000505         2492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1711272, whole genome shotgun sequence.
ACCESSION   DASRJQ010000505 DASRJQ010000000
VERSION     DASRJQ010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1711272"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..554)
                     /locus_tag="VFB89_11610"
     CDS             complement(150..554)
                     /locus_tag="VFB89_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM27998.1"
                     /translation="MRVASAALLALLIGSTACRSYEAYPKLASQKGLVPADQFAGYGR
                     EQAEAVAIGRELAAAHHGESPDALSRQAAQATAYAKSLPDVVDVTPDAPGHRLTVRFK
                     SGWRAGIVPIEDGKRGSETPGLPAGAGAATPK"
     gene            645..1454
                     /locus_tag="VFB89_11615"
     CDS             645..1454
                     /locus_tag="VFB89_11615"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZM27999.1"
                     /translation="MKFRNAPLARTVLLAGGLVVPVCARAQTESDSTFNPAPPDSIAS
                     YIPHSAPIESHAIRWYEPLAVVGGIGLLSALDQPVADHFRFHRSTTGQNVADTWARVG
                     TPIFYAPVTLGVITGGLISRNREVTQTGMRLAFSLLLAGVADQGLKMVFGRERPDANF
                     GAFDFDPIHPDAAFPSGHTAMAFAMATSLADDLHPTWAKVGLYGLATGVAVSRVYQQR
                     HWMSDVLGGAVVGVASAKFVSGRWTIFGLRAPSFLIKGGAPALGWQFSFHE"
     gene            complement(1540..>2492)
                     /locus_tag="VFB89_11620"
     CDS             complement(1540..>2492)
                     /locus_tag="VFB89_11620"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HZM28000.1"
                     /translation="LATTLPTTRLAAQQGRDSSYQAPPSSGGPTLKVGGYIQARETWQ
                     EAGNVLTGTLNRARAYVEGNLTAGFTYRIMAEYQAGGNASTAASVSLRDAYIRWTHND
                     FVVWAGQFKTPFSPEFIMSITQVETADRATVVDTLAPKRDIGVMATYAFRPYVTLAGG
                     FFNGEGQNRIINVDSANMVVARATVRPIPFITFGGNVAAYASDSTRWGVDAQLEYRGA
                     LVRAEYIWQDHHASLPNDEGWYGLAAYRVLPWVQLVFRQEQFARDAISLDRHVVATTG
                     GVNVEFAAARVRLLVNYVSRKIGEPGVRNSTVIGQAQVRF"
BASE COUNT          412 a          814 c          795 g          471 t
ORIGIN      
        1 taggtgctgg cgatcgggcc ggagatctga gcgcacgcag ccagccgctc ctcgacgagg
       61 acggctgcaa gctgctccgc cgcttcgcgg gaatcgatcg tggtggtcac ctggcaaaag
      121 tcagtcatgc ggtgcccacc agtgagggtc tacttcggag tcgccgcccc ggcgcctgcc
      181 ggaaggcccg gcgtctcgga gccgcgcttg ccgtcttcga tcggaacaat gccggcgcgc
      241 cagccgctct tgaaccggac cgtgagacgg tgcccgggcg cgtcaggggt gacgtcgaca
      301 acgtccggaa ggctcttggc gtacgcggta gcctgcgctg cctggcggga gagcgcatcg
      361 ggggactccc catggtgggc cgccgcgagc tcccgtccga ttgccacggc ttctgcttgc
      421 tctcggccat acccagcaaa ctgatcggcc ggcaccaggc ctttttgact cgccagcttg
      481 ggatatgctt catacgaccg gcaggcagtg gaaccgatca gtagcgccag caacgctgct
      541 gaagcaacgc gcatcgtctt cacctaggtg gcaggcccgc ctaatataca ccgacctcac
      601 gccgtgctgt cccctgattg cggcgtgttc cggaggaaag agccttgaag ttccgcaatg
      661 cgccgctcgc gcgcacggtt ttgctggcgg gcggcctcgt tgttcccgtc tgcgctcggg
      721 cccagaccga gtccgactcg acgttcaatc cggcaccgcc cgattcgatt gcttcataca
      781 taccgcatag cgctcccatc gagtctcatg cgatccggtg gtacgagccg ctcgccgtgg
      841 ttggcggcat tggactgctc tccgcactgg atcagcccgt cgccgaccat ttccggtttc
      901 accggtcgac caccggccag aacgttgccg acacctgggc ccgggtagga accccgatat
      961 tctacgcgcc ggtaacgctc ggtgttataa cgggcggact cataagccgg aatcgagaag
     1021 tcacgcagac gggtatgcga ctggcgtttt cgctcctcct cgcgggcgtt gccgatcagg
     1081 gtctcaagat ggtgttcggt cgcgagcggc cggacgccaa cttcggcgct ttcgacttcg
     1141 acccgattca tccggatgca gcgtttccgt cgggtcatac cgcgatggcc ttcgcgatgg
     1201 caacttcgct ggcggacgac ctgcatccga cgtgggccaa ggtcggtctg tacgggctgg
     1261 cgaccggcgt cgcggtgtcg cgggtctacc agcaacgaca ctggatgtcc gacgttctcg
     1321 gaggggcggt ggtgggcgtt gcgtcggcca agttcgtgag cggacgctgg acgatttttg
     1381 gccttcgagc gccgtcgttc ctcatcaagg gcggagcgcc ggcgctgggt tggcagttct
     1441 ctttccacga atgacgccag ggggcaggct cgcttatcga tcctgccacc cccaattctc
     1501 gttaccttcg tgggaacgcg ggcagtggca acgcctcggt caaaaccgga cctgcgcctg
     1561 accaatgacc gtactgttcc gcaccccggg ctcaccgatt ttccgcgaga cgtaattcac
     1621 gagcagccgc acccgcgcgg ctgcaaactc gacgttcacc ccgccggtgg tggcgaccac
     1681 gtgccgatcg agactgatcg cgtcgcgcgc gaattgctcc tgccgaaata ccaactggac
     1741 ccaaggcagt actcgatagg ccgcgagccc gtaccagccc tcgtcgttgg gcagcgaagc
     1801 gtgatggtct tgccagatgt attcggcacg caccaatgca cctcgatact cgagctgagc
     1861 gtcgacgccc cagcgcgtgc tgtcgctggc gtacgccgcg acgtttccgc cgaacgtgat
     1921 gaacgggatg ggacggacgg tcgcccgggc caccaccata ttcgccgagt cgacgttgat
     1981 gatccgattc tggccttccc cgttgaaaaa tccgccagcc agggtgacgt agggccgaaa
     2041 ggcatatgtt gccatcacgc cgatgtcccg cttgggcgcg agtgtatcga ccacggtggc
     2101 gcggtcagcg gtctcgacct gggtgatcga catgatgaac tcggggctga acggggtctt
     2161 gaactgcccc gcccagacca caaaatcatt gtgggtccac cgaatgtacg cgtcacgaag
     2221 gctcacactc gcggcggtgc tggcgttccc gccggcctgg tactccgcca tgatccgata
     2281 ggtgaaccct gccgtcagat tgccctcgac gtaggcgcgc gcccggttga gcgtgcctgt
     2341 cagaacgttg cccgcctcct gccaggtttc ccgggcctgg atatatccgc ccaccttgag
     2401 cgtgggcccg ccggacgatg gtggcgcctg ataagacgaa tcccgacctt gctgcgcggc
     2461 gagtcgggtc gtgggcaagg tcgtggccag ga
//
LOCUS       DASRJQ010000506         2901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3738021, whole genome shotgun sequence.
ACCESSION   DASRJQ010000506 DASRJQ010000000
VERSION     DASRJQ010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3738021"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VFB89_11625"
     CDS             <1..496
                     /locus_tag="VFB89_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZM28001.1"
                     /translation="EFRRGAERKTVSLITAGDSKSFEMGSRVVLPRDLGQQNYDAMRA
                     MGSDPDIGDLDIRLPRLAMLYGGELSELELAPLNPDLGQYFGTSEGILVISAPRKSSL
                     GLRGGDVVLAVDQRKPASPSHLLRILRSYDRDESFKIDIMRNRKHETVTAKRDDSNRP
                     DDSK"
     gene            complement(502..2220)
                     /gene="aroF"
                     /locus_tag="VFB89_11630"
     CDS             complement(502..2220)
                     /gene="aroF"
                     /locus_tag="VFB89_11630"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HZM28002.1"
                     /translation="MRVAIQGTVGSFSEAAGRRRWPELEPAACREVQDVVTAVRDGSA
                     DAGILPIENSLIGSVTSTYDLLQEAFGDGDLRLTHEIMLPVHHTLMGLPGADLSRIRR
                     VLSHSVALGQCRIWLGKHLPGAELVSAWDTAGSAEIVARERNPALAAIAARHAADAHG
                     LVPLAERIEDDPSNQTRFLTFTRADAAIPQSPSGPFKTSLIVCVDHKPGSLALTLQAF
                     GVRGVNVTALQSRPERSKPWTYRFYVDVAGASSDPRVAEALEEIEALAVELVILGSYE
                     AWPDGALRFALPVPTPAHYVRKPEVPLVDRRRNPNGTVVTVGDLSFGAEAPILIGGPC
                     SVETEAMILETAMVVANAGGDMLRGGAYKPRTSPYDFQGLGVKGLRYLAQAREATGLP
                     VVTEVLSWEEVPLVAHFADMLQIGARNMQNFSLLRAASRSGKPILLKRGAGATIEEWL
                     MAAEYVLAEGNPNVVLCERGIRTFERATRHTLDLNAVALVRERTHLPVVVDPSHAAGI
                     SSLVAPLGMAALAAGASGLIVEVHPSPATALSDGPQSLDPSQFAALARQVHPERSQKA
                     KVQVTA"
     gene            2515..>2901
                     /locus_tag="VFB89_11635"
     CDS             2515..>2901
                     /locus_tag="VFB89_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28003.1"
                     /translation="MGLGSAALNTGACLPGYCTRLLYAIGLSWAGFAVPAPAQSLPQY
                     APINPVALSRSGVYFQPYRNPRLGHLGFDVRLDYGSAIEYNIPSPRPSYLLDGEFLRL
                     DVTATRDLNERIFLFGTVGVGGGYAGF"
BASE COUNT          499 a          955 c          919 g          528 t
ORIGIN      
        1 cgagttccgt cggggcgcgg agcgcaagac cgtctccctc atcaccgcgg gcgattccaa
       61 gtcattcgag atgggaagcc gggtcgtctt gccgcgtgac ttggggcaac agaactacga
      121 tgccatgcgg gcgatgggct cggatccaga catcggtgat cttgatatcc ggctccctcg
      181 ccttgcgatg ctttatggcg gagagctctc tgagctcgag ctcgcgccgc tcaatcccga
      241 ccttggccag tatttcggga ccagcgaggg cattctggtg atcagcgcgc cccgcaagtc
      301 ctcgctgggc ctcaggggcg gtgacgtcgt tctcgcggtg gatcagagaa aacccgcgag
      361 tccgtcccac ctgctacgaa tcctgcgaag ctacgatcgc gacgaaagct tcaagatcga
      421 catcatgcgc aaccggaaac acgagaccgt caccgccaag cgggacgact ccaaccgccc
      481 ggacgactcg aagtagtagc ctcaagcggt tacttgcact ttcgccttct gcgacctttc
      541 cggatgtacc tgccgggcca gcgctgcgaa ctggctcgga tcgaggcttt gcggcccgtc
      601 gctcagcgcg gttgccggtg aggggtggac ctccacgatc agtccgctgg cgccggccgc
      661 cagcgcggcc atgcccaacg gtgcgaccag tgagctgata ccggccgcat gactgggatc
      721 gaccacgacg ggcaggtggg tgcgctcccg aaccaatgca accgcattca gatcgagggt
      781 atgacgagtg gcccgctcga acgtgcggat gccgcgttcg cagagcacca cgttgggatt
      841 gccctcggcg agcacgtact ccgcggccat cagccattcc tcgatggttg cgcctgcccc
      901 gcgcttgagc aggatcggct ttccgctccg gctcgccgct cgcaataacg agaaattctg
      961 catgtttcga gcgccgatct gcagcatatc ggcgaagtgg gctacgaggg ggacttcttc
     1021 ccaactcaac acttcggtga cgaccggaag tccggtggcc tcccgcgctt gcgccaggta
     1081 ccgaaggcct ttcacgccga ggccctgaaa atcatagggt gacgtgcgcg gcttgtaggc
     1141 cccgcctcga agcatgtctc ccccggcgtt ggccactacc atggcggtct ccaggatcat
     1201 cgcttcggtt tcgaccgagc atggcccgcc gatcaggatc ggcgcctccg ccccgaagga
     1261 cagatcgcct acggtcacaa cggttccatt cggattgcgc cgacgatcca cgagagggac
     1321 ctccggcttc cgaacgtagt gcgctggcgt ggggaccggt aacgcaaaac ggagcgcccc
     1381 atccggccac gcttcgtagc tgccgagaat caccagctcg accgcgagcg cctcgatttc
     1441 ttctagggcc tcggctactc gaggatccga cgaggcaccg gcgacgtcta cgtagaaccg
     1501 gtacgtccac ggcttggagc gctccggccg agactggagc gccgtcacgt ttacccctcg
     1561 aacaccgaac gcttgcaggg tgagcgccag gctccccggc ttatgatcga cgcagacaat
     1621 cagcgaggtc ttgaagggtc cgctcgggct ctgcggaatc gcggcgtcgg cccgagtgaa
     1681 tgtcaggaac cgggtctggt tggacggatc atcctcgatc cgctcggcca gcggcaccag
     1741 tccgtgcgcg tccgcggcgt ggcgggcagc aatggcggcg agtgccggat tgcgctcccg
     1801 ggccacgatc tcggcgctcc ccgccgtgtc ccacgcgctc accagctccg cgccgggcag
     1861 atgttttcct aaccagatcc ggcattgtcc cagcgccacc gaatgggaca ggacccgccg
     1921 gatccggctc aggtctgcgc cagggaggcc catgagcgtg tggtgaacgg ggagcatgat
     1981 ctcgtgagtg agacggagat ccccatcgcc gaaggcttcc tggagcaggt cgtaggtgga
     2041 cgtgaccgag ccgatcaggg agttctcgat gggcagaatc cccgcatcgg cggaaccgtc
     2101 ccggaccgcg gtcacgacgt cttgaacctc gcggcacgcg gccggctcga gctcgggcca
     2161 tcgacggcgg ccggcagcct cgctgaagga gcctacggtc ccctgaatcg ctactcgcat
     2221 gtcgtcgact ccttcgagac acaggaaaaa aaagcccgcc gtctcggcgg ggcatcgatc
     2281 ggtccgttct ggcctgagcg tcagtgcacg ctggtgccgg aaccggacgg ccgttgcgcc
     2341 gcgccggtgc gagcaaagta gaaataccag aatgcacgct gcatgatcat gcgatgagtc
     2401 tacgatccgg tcggccggtc tgtcaagcag ggttcgcggc tcgggttgga atcgggatcg
     2461 cttatgtttc gccgacccga ctgaccgccc gccctcgctt ttgggagctc ggccttggga
     2521 ctcggatccg cggccctgaa cactggtgcg tgcctcccag gctattgcac ccggctcctt
     2581 tacgcgatcg gcctctcatg ggccggcttt gccgtgcctg ctccggccca gagcctgccg
     2641 caatacgcgc cgatcaatcc ggttgccctg tctcggagcg gcgtgtactt ccagccgtac
     2701 cgcaaccctc gactgggtca cttgggcttc gacgttcgcc tcgattacgg cagcgcgatc
     2761 gaatacaaca ttccctcgcc gcgaccgagc tacttgctcg atggcgaatt cctgcgtctc
     2821 gatgtgactg cgacgcggga tctgaatgag cggatctttc tgttcggaac cgtcggtgtc
     2881 gggggcgggt acgctggttt c
//
LOCUS       DASRJQ010000507         2001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3738680, whole genome shotgun sequence.
ACCESSION   DASRJQ010000507 DASRJQ010000000
VERSION     DASRJQ010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3738680"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1711
                     /locus_tag="VFB89_11640"
     CDS             <1..1711
                     /locus_tag="VFB89_11640"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM28004.1"
                     /translation="QATDEALDGRSDVYSLGCVLYEMLVGHAPFTQVNPVALLAARVT
                     SAAPTVRSSGVSVPAAVDHLVGRTLAQFREQRHQSAAELAVALSAAEQEIAYGTPTPG
                     TTQPVARVAALAVLPFVNMSNDPDNEFFSDGITEELINALARVKGLRVTSRTSVFAFK
                     GREQDVRDIGQRLNVSAVLEGSVRRAGNRLRVSAQLINAADGYHLWSESYDRQLADVF
                     EVQDELSRSIVNTLRPKLVGADSEPLVQPATASVEAYTAYLKGRFFWNKRTLDGYRRG
                     IEFFEQALTKDPDYALAYTGIADCWAMLAFDYFGGVPSAEGMPRAKAAALKALELDDS
                     LAEARSPLAVVAMLYDWDYVASEQHFKRALQIKPGYFPARLWYSFMLSVTGRHEEAIE
                     MINRTVELEPLSLIAHQAVARIHHYAGRDEEAVEHCRRLIEMDPSYVTGYETLTRPLV
                     MLGRYQEALEVALEGVERSGRWSLLLAALGHVYGRMGKREEALGILAELEAQARQRYV
                     PRYHIALVYYGLRDEADSLRELERSFQEHSGVIAWAKVDPHINWLMRNDRFRQILHQA
                     GLE"
     gene            1744..>2001
                     /locus_tag="VFB89_11645"
     CDS             1744..>2001
                     /locus_tag="VFB89_11645"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZM28005.1"
                     /translation="MDAMDTAWRYVDAWNARDTARLAASFREGGTYEDPNTGGPIGTA
                     GLSTYAAALFAAFPDLTFEDAGMRRVSENELHFAWIMKGTNR"
BASE COUNT          347 a          610 c          722 g          322 t
ORIGIN      
        1 acaggcgacc gatgaggcgc tggacggacg gagcgacgtg tactcgctgg gttgcgtgct
       61 ctacgagatg ctggtgggcc acgcgccgtt cacccaggtc aatccggtgg cccttctggc
      121 ggcgcgagtc acctcggctg cccctacggt ccggtcatcg ggcgtgtcgg ttccagccgc
      181 ggtggaccac ctggtcggac ggaccctggc gcagtttcgc gagcaacgcc atcagagcgc
      241 agccgagctc gcggtggcgc tgagcgcggc ggaacaggag atcgcctacg ggaccccgac
      301 cccgggcacg acccaaccgg tggctcgcgt ggccgcgctc gcggtgctgc ccttcgtcaa
      361 catgagcaac gacccggaca acgagttctt cagcgacggt atcactgagg agctgatcaa
      421 tgccctggcg cgagtgaagg gactgcgggt gacgtcacgc acctcggtgt tcgccttcaa
      481 gggccgggag caggacgtgc gagacatcgg gcagcggctc aacgtgagcg ccgtgctgga
      541 ggggagcgtg cgccgtgcgg gcaaccggct gcgggtcagc gcgcagctca tcaacgccgc
      601 ggacggatac catctctggt cggagtcgta cgatcgtcag ctggccgatg tgttcgaggt
      661 gcaggatgag ctgtcgcgat ccatcgtgaa tacgctgagg cccaagctgg tgggagcgga
      721 ctccgagccg ctggtgcagc cggccaccgc cagcgtcgag gcctacaccg cgtatctcaa
      781 ggggcgcttc ttctggaaca agcggacgct cgacggctat cggcggggca tcgagttctt
      841 cgaacaggcg ctcaccaagg accccgacta cgccctggcc tacaccggca tcgcggactg
      901 ctgggcgatg ctggcgttcg actacttcgg cggcgtcccc tcggcagaag ggatgcccag
      961 ggcgaaggcg gccgcgctca aggcattgga gctggacgac agcctagcgg aggcccgctc
     1021 accgctggcg gtggtcgcca tgctgtacga ctgggattac gtcgcgtcgg agcagcactt
     1081 caagcgcgcg ttacagatca agccagggta cttcccggcg cggttgtggt actcgttcat
     1141 gctgtcggtt accggccggc acgaagaggc gatcgagatg atcaaccgca cggtcgagct
     1201 ggagccgctc tccctcatcg cgcatcaggc ggtcgcccgg atccaccact atgcgggacg
     1261 ggacgaggaa gcggtggagc actgccgccg gctgatcgag atggatccga gctatgtgac
     1321 ggggtacgag acgttgacgc gacccctggt gatgctgggg cgctaccagg aggcattgga
     1381 agtggctctc gagggggtgg agcgctcggg tcgatggtcg ctgctcctcg cggcactggg
     1441 ccatgtgtac gggcggatgg gcaagcggga ggaggccctc gggatcctgg cagagctgga
     1501 ggcgcaggcg cgccagcgct atgtgccgcg gtatcacatc gcgctggtct actacggcct
     1561 gagggatgag gcggactccc tacgagagct ggagcgcagc ttccaggaac acagcggggt
     1621 catcgcctgg gccaaggtcg atccacatat caactggctg atgcggaacg atcgctttcg
     1681 ccagatcctg caccaggcgg ggctggagtg agcacgcgga acgccggctc agggaggacc
     1741 gccatggatg cgatggacac cgcgtggcgc tatgtggacg cgtggaatgc acgggacacg
     1801 gcgcgtctcg ccgcgtcgtt tcgggaagga gggacctacg aggaccccaa caccggaggc
     1861 ccgatcggaa cggccggcct gtcaacctat gcggccgcgc tctttgcggc gtttcccgat
     1921 ctcacgttcg aggatgcggg gatgcggcga gtgtccgaga acgagctgca tttcgcctgg
     1981 atcatgaagg gcaccaatcg g
//
LOCUS       DASRJQ010000508         2772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2214293, whole genome shotgun sequence.
ACCESSION   DASRJQ010000508 DASRJQ010000000
VERSION     DASRJQ010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2214293"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..>2772)
                     /locus_tag="VFB89_11650"
     CDS             complement(223..>2772)
                     /locus_tag="VFB89_11650"
                     /inference="COORDINATES: protein motif:HMM:NF019156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HZM28006.1"
                     /translation="QWMGHVYQYLGLEVACLDDTEPSSPERRAAYQADITYGTNNEFG
                     FDYLRDNMVFSLEQRVQREHAYAIIDEVDSILIDEARTPLIISGPVGNETDDKYAQFN
                     RQVAELVRKQTAVVNTLLAEAEKLLEDEKTRQDAALKLYQAHLGMPKNKRLLKMLQEH
                     GIKQLVQRMELDAIADRKLPMRQQRMRDTEDVLYFVLDEKGHSVHLTDRGAETMSPGD
                     PTLFLVPDISEEIHRIDKDPELSPHDRIERRRTVESEYALKSEKLHIIHKLLQAHVLY
                     EKEVDYLVQEGQVMIVDEFTGRVMTGRRWSDGLHQAVEAKEGVQVKGETQTFATITIQ
                     NYFRMYDKLAGMTGTAETEETEFYQIYGLEVNVIPTHRPVCRIDQVDRIYKTRREKYN
                     AVLDEVERLHNLGWPILIGTVTVEVSETLSRQLKRRGLKHEVLNAKYHQREAEIVAQA
                     GQRSSITIATNMAGRGTDIKLGKDLDLTTEEAGLQILGTERHESRRIDRQLRGRAGRQ
                     GDPGASLFFLSLEDDLMRLFGSDRIARYMDKTGAEEGEVITGMLVMKAIESAQKRVEL
                     QNFQSRKRLLEYDDVMNQQREVAYSLRLFSLERGEELKAEARQMISSALDHAVRKFLA
                     EFDRPEDWDRKALIDALSLSFLVTPDAIGDAAAVKDVDAAVKAAQAEGEAAFHRKVDY
                     LREFGRGIGIADVDQQILSQVMLSVIDEKWKDHLYDLDQLRNAIQYRAYGQKDPLVEY
                     KKEAYEMFEDLMRDIQATFTERYLKITVTAEPPRQPPPPPIPTPRSGTDELFAGASRT
                     RTGPMGGPPPRVETPLGRVGSPSGGVPEVGRNDPCPCGSGKKYKKCHGAGK"
BASE COUNT          403 a          940 c          887 g          542 t
ORIGIN      
        1 aatgtttccg ccccctggga ccagaggcgc tcgcggggac ggtcgggtcg ggaggcggga
       61 ggagcgatgg ggagggccat ccccggagac tagagccgcg gaactcggac gaagggtcga
      121 gatcgcgcgg gagggcgcga aaaagccgtg agaggtgaga cgtgagaggt gagacggacc
      181 agcctactca cgtcttacgt cctacgtctc aggtctcgcg cattatttcc ccgccccgtg
      241 gcacttcttg tacttcttcc ccgatccgca cgggcaggga tcattccgcc cgacctccgg
      301 cactccaccg gacggcgagc ccacccgccc cagcggggtt tcgacccgcg gaggcggccc
      361 gcccatcggt ccggtgcggg tgcgcgaggc ccccgcgaag agctcgtcgg tgccgctccg
      421 gggcgtgggg ataggtggcg gcggcggctg gcgggggggc tccgccgtga ccgtgatctt
      481 gagatagcgt tccgtaaagg tcgcctggat gtcgcgcatc agatcttcga acatctcata
      541 ggcttccttc ttgtactcga ccagcgggtc cttctgtccg taggcgcggt actggatcgc
      601 gttccggagc tggtcgaggt cgtacaggtg atccttccac ttttcgtcga tcaccgagag
      661 catgacctgg gagaggatct gctggtcgac gtccgcgatg ccgatccccc ggccgaactc
      721 gcgcagataa tccaccttgc ggtggaaggc cgcttcgcct tcggcctgcg ccgctttcac
      781 cgcggcgtcg acatccttca ccgccgcggc gtcgccgatg gcgtcgggcg tgaccaggaa
      841 ggagagcgag agcgcgtcga tcagcgcctt gcggtcccag tcctccgggc ggtcgaactc
      901 ggcgaggaac ttccgcaccg catggtcgag cgcgctggag atcatctgcc gcgcttcggc
      961 cttcagctct tcaccccgct ccagcgagaa gagccgcagc gaataggcga cttcgcgctg
     1021 ctggttcatc acgtcgtcgt actcgagcag ccgcttccgg gactggaagt tctgcagctc
     1081 gacccgcttc tgcgccgatt cgatcgcctt catcaccagc atcccggtga tgacttcgcc
     1141 ctcctcggcg ccggtcttgt ccatgtaccg cgcgatccgg tccgacccga acagccgcat
     1201 caggtcgtct tccagcgaca ggaagaagag cgacgctccc ggatcgccct gccgcccggc
     1261 gcgcccgcgc agctgccggt cgatccggcg cgactcgtgc cgctcggtgc cgaggatctg
     1321 gagcccggcc tcctcggtcg tgagatcgag atccttgccc agcttgatgt cggtaccgcg
     1381 gcctgccatg ttggtcgcga tcgtgatcga gctgcgctgt cccgcctgcg ccacgatctc
     1441 cgcctcgcgc tggtggtact tcgcgttcag cacctcatgc ttgagacccc gacgcttgag
     1501 ctgccgcgac agcgtctccg acacctccac cgtcaccgtg ccgatgagga tcggccagcc
     1561 caggttgtgc agccgctcga cctcgtccag caccgcgttg tacttctccc ggcgggtctt
     1621 gtagatccgg tccacctggt cgatacggca caccggccgg tgagtcggaa tgacgttgac
     1681 ctcgagtccg tagatctggt agaactcggt ctcctcggtc tccgcggtac cggtcatgcc
     1741 ggccagcttg tcgtacatcc ggaagtagtt ctggatagtg atggtggcga aggtctgggt
     1801 ctcccccttc acctgcacgc cttccttggc ctccaccgcc tggtgcagcc cgtcggacca
     1861 gcgccgcccc gtcatcaccc gcccggtgaa ctcgtccacg atcatcacct ggccctcctg
     1921 caccaggtaa tccacttcct tctcgtagag gacgtgcgcc tgaagcagct tgtggatgat
     1981 gtggagcttc tcgctcttga gcgcgtattc cgactcgacg gtccggcgcc gctcgatccg
     2041 gtcgtgcggc gagagctcgg ggtccttgtc gatccggtgg atctcctccg agatgtcggg
     2101 caccaggaag agcgtcgggt cgcccggcga catcgtctcc gcaccgcgat cggtcagatg
     2161 gaccgagtgg cccttctcgt cgagcacgaa gtagagcacg tcctcggtgt cgcgcattcg
     2221 ctgctgccgc atcggtagct tccggtccgc gatcgcgtcc agctccatgc gctggaccag
     2281 ctgcttgatc ccgtgctcct gcaacatctt gagcagccgc ttgttcttcg gcatgccgag
     2341 atgggcctgg tacagcttca gcgcggcgtc ctgccgggtc ttttcgtcct ccagcagctt
     2401 ctcggcctcg gccagcagcg tattcaccac ggccgtctgc ttccgcacca gctccgccac
     2461 ctgccggttg aactgggcgt acttgtcgtc ggtctcgttg ccgaccggcc cggagatgat
     2521 gagcggcgtg cgcgcctcgt cgatgaggat cgagtccacc tcgtcgatga tggcgtacgc
     2581 gtgctcccgc tgcacccgct gctccagcga gaacaccatg ttgtcgcgca ggtagtcgaa
     2641 gccgaactcg ttgttggtgc cgtaggtgat gtcggcctgg taggcggcgc ggcgctcggg
     2701 ggaggacggc tcggtgtcgt cgaggcaggc gacctcgagg ccgaggtact ggtagacatg
     2761 ccccatccac tg
//
LOCUS       DASRJQ010000509         2459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2588311, whole genome shotgun sequence.
ACCESSION   DASRJQ010000509 DASRJQ010000000
VERSION     DASRJQ010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2588311"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..436
                     /locus_tag="VFB89_11655"
     CDS             20..436
                     /locus_tag="VFB89_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28007.1"
                     /translation="MIARAFTVLSAAVILALGVVHLLYTFRGPKLMPRDRALQARMRE
                     VSPVISRETTMWKAWIGFNASHALGAILFGLVYGYLALAHPAVLFQSAFLLGLGLCLL
                     AAYTALGKAYFFSVPFTGILIALVSYVIGLVAALSR"
     gene            complement(453..872)
                     /locus_tag="VFB89_11660"
     CDS             complement(453..872)
                     /locus_tag="VFB89_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28008.1"
                     /translation="MYEVEELVVSPDKKWLAVVSVGEGHPALEVVDAQTLLLQRKYIP
                     VHAIDPYPGTVSVTKWEGDSLLVTSDMPLHLSEGSRLDPELMVLEQQEFRLFVGSWHV
                     EPKDWDKLPRDWRTPNTPLERALLRQLLPRSSRSGRE"
     gene            complement(1236..1655)
                     /locus_tag="VFB89_11665"
     CDS             complement(1236..1655)
                     /locus_tag="VFB89_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HZM28009.1"
                     /translation="MSTPKTLYRKIFGSRTGIRLLLPLLGLALACGVGLSARTGSREA
                     RVITLTARDMAFYQSGDPTPNPTLVVRRGERVRLTLTNEDRGMTHDFAVRSMDVATRA
                     LRGGTDSLVFEAPERPGESEYVCTYHARMMKGVLKVE"
     gene            complement(1667..>2459)
                     /locus_tag="VFB89_11670"
     CDS             complement(1667..>2459)
                     /locus_tag="VFB89_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28010.1"
                     /translation="PRPGWRFFWLFALIGVYVGVIPVALGLLWYPLVGRLGPRALGFL
                     LALTIGLLLFLLVDTAHEGLEAVEGMPAAFQGIALFVFGIVAAYLGIEAFGGWLAKRR
                     SGAGAVSAGSAGWATALLVAIGIGLHNLGEGLAIGAAIGLGEVALGRLLILGFTLHNT
                     TEGLAIIAPIARTPVSLGRLLVLGLVGSLPTIAGAWLGGFLYSPTLSVLFLALGAGAI
                     AQVIVQIAKGMAAGRPLADALRSGPVMAGLIAGFALMYTTGMLIQ"
BASE COUNT          441 a          835 c          762 g          421 t
ORIGIN      
        1 tttccgatag tgtggaccga tgatcgccag agccttcacg gtcctcagcg ccgccgtgat
       61 cctggcgctc ggggttgtgc acctcctcta cacctttcga ggtccgaagc tcatgccgcg
      121 cgatcgcgcg ctccaggcca ggatgcgaga ggtctcgcct gtgatcagcc gcgaaacgac
      181 gatgtggaag gcatggatcg gcttcaacgc gagccatgcc ttgggagcta tcctcttcgg
      241 gctcgtctat ggctacctgg cactcgcgca cccggccgtc ctgttccagt cggctttcct
      301 gctcggcctg ggcctctgtc tgctcgccgc ctatacggct ctcggaaaag cctacttttt
      361 cagcgttccg ttcacgggga ttctgatcgc gctcgtctcg tatgtcatcg gcttggtggc
      421 agcgctgagc cgctaatcgc gccggcccag gctcactcac gacctgaacg gctcgacctc
      481 ggcaagagtt gccggagcaa cgctcgctcg agcggggtgt tgggggtccg ccaatctctt
      541 ggcaacttgt cccaatcttt aggttcgacg tgccatgaac cgacgaagag gcggaattct
      601 tgttgctcta gcaccataag ttcagggtcc agccggctgc cctcactcag atggagcggc
      661 atgtcgcttg tgactagcag agagtcacct tcccatttag tcaccgagac ggtaccagga
      721 tatggatcaa tcgcgtgaac cggtatgtac ttgcgttgca ggagcagagt ctgtgcatca
      781 acgacttcaa gcgcagggtg cccctcgccc accgacacca cagcgagcca ctttttgtcc
      841 ggtgaaacca ccaactcttc aacttcgtac atatcactta ggccgtaacc aagaaactgg
      901 tcgccttcat tctgacagcg aagcaaccaa cgggctcccc tggcgtggcc tcggtttttt
      961 acgacgaccg tgcagtgagg atctagtgct gcagctactt cctgtgagtc ctccgcgttg
     1021 tcgcttatag cggctacgcc ttgtgcgggg caggatatag agccgcaaac gaccaacagg
     1081 atcacgggat tcaccttccc accaaaacct cgaagccaga tagacatgta ggctctaaga
     1141 ggtgtaaaca acccccattg tcgttgcgtg ttcatagatc gttctccccc tcgcccaacg
     1201 tgactgggcc accagccgtg gagcgcgggt cgggtctact cgaccttcaa gacccctttc
     1261 atcatccgcg cgtggtaggt gcagacgtac tcgctctcgc ccggccgttc gggcgcttcg
     1321 aagaccaggg agtcggtgcc gccccgcagg gcgcgcgtgg ccacgtccat cgacctgacc
     1381 gcgaagtcgt gcgtcatgcc ccggtcctcg ttggtgaggg tgagccgcac cctttcgccg
     1441 cggcgcacga ccagggtcgg gttcggcgtc gggtccccgg actggtagaa cgccatgtcg
     1501 cgggcggtca gggtgatgac gcgcgcctcc cgggagccgg tgcgcgccga aagaccgacg
     1561 ccgcaggcca gcgccaggcc gagcaacggc agcaacagcc ggattcccgt gcggctgccg
     1621 aagatcttgc ggtagagggt cttgggagtg ctcatggtcg gtaacctcac tggatcaaca
     1681 tgcccgtggt gtacatcagc gcgaagccgg cgatcagtcc ggccatcaca ggtccgctcc
     1741 gcagggcgtc ggcgagcggg cggccggcgg ccatgccctt ggcgatctgc acgatcacct
     1801 gcgcaatcgc gccggccccc agggccagga acaggaccga cagggtcggc gaatagagga
     1861 agcctccgag ccaggcgccc gcgatcgtgg gcaggctccc caccagcccc agcacgagca
     1921 ggcggcccag ggagaccggg gtccgggcga tcggggcgat gatcgccagc ccctcggtcg
     1981 tgttgtgcag cgtgaagccc aggatcagca gccgtcccag ggccacctcc ccgaggccga
     2041 tcgccgcgcc gatcgccagg ccctcgccca ggttgtgcag gccgatgccg atggcgacca
     2101 gcagcgccgt cgcccatccc gccgatcccg ccgataccgc gcccgccccg gaccggcgct
     2161 tcgccagcca gccgccgaac gcctcgatcc ccaggtaggc ggcaacgatt ccgaagacga
     2221 acagggcgat cccctggaac gcggccggca tcccttccac cgcttcgagc ccctcgtgcg
     2281 cggtgtcgac cagcaggaag agcagcaggc cgatggtgag cgccagcagg aagcccaggg
     2341 cgcgcgggcc gagccggccg accagcgggt accagagcag gcccagggcc accgggatga
     2401 ccccgacata cacgccgatg agcgcgaaca gccagaagaa ccgccacccc ggccgcggc
//
LOCUS       DASRJQ010000510         3738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2144408, whole genome shotgun sequence.
ACCESSION   DASRJQ010000510 DASRJQ010000000
VERSION     DASRJQ010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2144408"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1230
                     /gene="lysS"
                     /locus_tag="VFB89_11675"
     CDS             <1..1230
                     /gene="lysS"
                     /locus_tag="VFB89_11675"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HZM28011.1"
                     /translation="VKLLDLDDHIGVSGRLFRTRMGEITIRVEAFELLAKSLRPLPRG
                     KSYVREDGSAVDTALADPEVRYRQRYADLAVHEEVRAIFQTRARAIAYLRRFLDDLGF
                     LEVETPILQPLYGGATARPFVTHHNALDMPLYLRIADELYLKRLLVGGLERVYEIGHD
                     FRNEGMDRTHNPEFTMLEFYQAYADYEEMMTTVEAMISGVVEHCCGSLTLERGGTTLD
                     FTPPWPRVRFVEALRERAGVDILTASDVDLRRALARMPGGSEQAADEIAGYSRPKLMD
                     ELFKEFIERRLVQPTFVVDHPKALSPLAKAHRGDDRLVERFELIVQGVELANAFSELN
                     DPDDQRARFEDQGRQREAGDDEAQRFDADYIRALEYGMPPAGGVGIGVDRLVMLLTNQ
                     PSIRDVILFPAMRPEVG"
     gene            1227..2555
                     /locus_tag="VFB89_11680"
     CDS             1227..2555
                     /locus_tag="VFB89_11680"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM28012.1"
                     /translation="MSTSQAAAPAETSVAGVAEYRAILRWPTRLERRIAGRYLRSRRA
                     KRAASLNTIIATGGVAVGVMALIVVLGVMNGLRDDLRERILVASPHLRVLTFGAGLRL
                     DDWQRVLATVRQQPGVIAAAPEVVSQAGISAGHDYGEGVNLLGFEPDTGSRSVTSFPQ
                     SIKKGDLSFRPDRKDVDGAILLGTRLANRLTVYVGDVVTLVPVTQAKMNPALGVAVPK
                     FWKFEVTGLFETGMFQYDNQFVVVSRSVAEQFTGLGDAVSGIAVRVADPEMAPEVGRE
                     LEQRLGYPYRTLDWQTQNASLFSALELEKLAMGLIIFFIMVVAAFNIVGTLTMVVTDK
                     TREIGILQAMGLTAPAVARIFLAQGAIIGIVGTMLGLAGGLVVAYVVDRSGWIRINPA
                     VYFIDHLPVHVELLDVTVVVLASIAVALLATLYPSRSAARLTPVEAIRHE"
     gene            2567..3265
                     /locus_tag="VFB89_11685"
     CDS             2567..3265
                     /locus_tag="VFB89_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZM28013.1"
                     /translation="MPILEATGLRKVYRGGDGSPIDVLTGADLAVGRGEFVAIVGASG
                     AGKSTLLHLLGALDRPTAGSVRLDGQAYDEMTAAELASVRNRKLGFVFQFHHLLREFT
                     ALENVMLPLLIAGATEATARSRAEELLARVGLAGRMTHRPTALSGGEQQRTAVARALA
                     TDPVVVLADEPSGNLDYGHSEVLHELFASLAREFETALVVVTHNRLLAVRADRVLSME
                     NGRLVPLTSVESMP"
     gene            3265..>3738
                     /locus_tag="VFB89_11690"
     CDS             3265..>3738
                     /locus_tag="VFB89_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28014.1"
                     /translation="MSCDQCHEREAVIHLTQIVNEQVTTVHLCERCAAEKGVESAASV
                     EQNPFAKLVAASMGKTTPDLSLVGSGTRGACPRCGATLQDFRESGRLGCADCWRAFEA
                     PLRDLLRRLHGSTVHMGERYADRVGAPVTANGDKAPRQADLREQLRLAIETENFEL"
BASE COUNT          647 a         1119 c         1273 g          699 t
ORIGIN      
        1 gtgaagctgc tcgacctgga cgaccatatc ggcgtgagcg ggaggttgtt tcggacgcgg
       61 atgggtgaga tcaccatccg ggtcgaggcg tttgagctcc tcgccaagtc gctgcggccg
      121 ttgccgcgtg gcaagagcta cgtgcgggag gatggcagcg cggtcgacac tgcattggcc
      181 gaccccgaag tccgataccg gcaaaggtat gccgacctgg cggttcacga ggaggtgcgg
      241 gcgatcttcc agacccgcgc gcgcgcgatt gcgtacctgc ggcgatttct cgatgacctt
      301 ggctttctcg aagtagagac tccgattctc cagccgctct atggcggagc aaccgcacgc
      361 ccgtttgtga cccaccacaa cgcgctggac atgcctttgt acctacgtat cgcagacgag
      421 ctctatctca agcgattgct ggtcggcggg ctggagcggg tctacgagat cggtcacgac
      481 ttccggaacg aggggatgga tcgcacccac aaccccgagt tcacgatgct cgagttctac
      541 caggcctacg ccgactatga ggagatgatg acgacggtgg aggccatgat ctccggggtt
      601 gtggagcatt gttgcggatc gctcaccctg gagcgagggg ggacgacact cgactttacg
      661 ccaccatggc ccagagtccg attcgtggag gcgctgcggg agcgggcagg ggtggacatt
      721 ctgaccgcct cggacgtcga cctgcgtcgg gcgctcgcac gcatgccagg gggatccgag
      781 caggccgccg acgagatcgc ggggtattcc cgcccgaagc tcatggacga gctgttcaag
      841 gagttcatcg agcgtcggct ggtacagccg acgttcgtgg tcgatcatcc caaggccctc
      901 tcaccattgg ccaaggcgca ccggggcgac gatcgtctgg tggagcgctt cgagttgatc
      961 gtgcagggag tcgaactggc caatgcgttc agcgagctga acgatccgga cgaccagcga
     1021 gcccggttcg aagaccaggg tcgccagcgg gaggcggggg atgatgaggc gcaacgcttc
     1081 gacgcggact atatccgcgc gctggaatac gggatgcccc ccgcgggagg tgtcgggatc
     1141 ggggtggacc ggttggtcat gctgctcacc aatcagccgt cgatccggga cgtgatcctg
     1201 tttccggcca tgcgaccgga agtcggatga gtaccagcca agcggcggca ccggccgaga
     1261 cctctgtcgc cggcgttgca gagtaccgcg ccattctgcg ctggcccact aggctcgagc
     1321 gccgaatcgc cgggcggtac ctgcggtcga ggcgggccaa gcgggcggcg tcgctcaaca
     1381 ccatcattgc aaccggcggc gtcgcagtcg gcgtcatggc cctcatcgtt gtgctgggcg
     1441 tgatgaatgg cctccgcgac gatctccggg aacgcattct ggtggcgagc ccgcatctgc
     1501 gagtgctgac cttcggcgcg gggctccggc tggatgactg gcaacgagtt ctcgccaccg
     1561 tgagacagca gcccggcgtc atagcggctg ccccggaagt ggtcagtcag gccggaatca
     1621 gcgccggcca cgactacggc gagggcgtaa acctcctggg ctttgagccg gacaccggct
     1681 ctcgttcggt cacctcgttc ccgcaatcaa tcaaaaaggg tgacctttcc ttccgtcctg
     1741 atcgcaagga cgtggatggt gcgattctgc tgggcactcg tcttgccaac cgactcacgg
     1801 tgtatgtggg cgacgtagta acactcgtgc cggtgactca ggcaaagatg aatccggcgt
     1861 tgggcgtcgc cgtccccaag ttctggaaat tcgaggtcac cggtctgttc gagaccggga
     1921 tgttccagta tgacaaccag ttcgtggttg tatcccgttc ggtcgctgag cagttcaccg
     1981 gcctgggcga cgcggtgtcg ggcatcgcgg tgcgagtggc ggatcccgag atggccccgg
     2041 aggtcggacg ggagctcgag cagcgcctcg ggtatcccta tcgtactctc gactggcaga
     2101 cgcagaacgc gagccttttc agcgccttgg agctcgaaaa gctcgccatg ggcctgatta
     2161 tctttttcat catggtagtc gcggcgttca atatcgtcgg gacattaacc atggtggtga
     2221 ccgacaagac ccgggaaatt ggaattctgc aggccatggg gctcacggcc cctgcagtag
     2281 cccgcatctt tcttgctcaa ggcgctatca tcggaatagt cggcacgatg ctcggactgg
     2341 cggggggatt ggttgtagcc tatgtggtag accgatcggg ctggattcgc atcaatcccg
     2401 ccgtctattt catcgatcac ctgccggtgc acgtcgagtt gctggatgtg acggtcgtgg
     2461 ttctcgccag catcgcggtc gcccttttgg caaccttgta tccatcgcgt tcggccgctc
     2521 ggctcacgcc ggtcgaagcg atccggcacg agtaggagac cctcgggtgc cgattctgga
     2581 agccaccgga ctccggaaag tctaccgggg cggcgacggc agcccgatcg acgtgctgac
     2641 tggcgccgac cttgcagttg gtcgcggtga gttcgttgca atcgtgggcg ccagcggggc
     2701 cggcaagagc acgctgctcc acctcctggg cgcgctcgat cggccaaccg ccgggagcgt
     2761 gagactggat gggcaggctt acgacgagat gacggcagcc gagcttgcct ctgtgcggaa
     2821 ccgaaagctt ggtttcgtct ttcagttcca tcacctgctg cgcgagttta ccgccctcga
     2881 gaacgtgatg ctgccattgc tcatcgccgg tgcaaccgag gccacggcgc gctcgcgggc
     2941 cgaggagctc cttgcccggg tcggactggc cggacgcatg acgcatcggc cgacggcact
     3001 ttccggcggc gagcagcagc ggacggccgt ggctcgggct ctcgccaccg atccggtcgt
     3061 ggttctcgcg gatgagccgt ccggcaacct cgactatggt cacagtgagg tgctgcacga
     3121 gctcttcgcc agtctggctc gcgagttcga gacggcgctg gtcgtggtga cccacaatcg
     3181 tctgctggcg gtgcgagcgg accgagtgct gagcatggag aatgggcggc tggtcccgtt
     3241 gaccagcgtg gagtcgatgc cctgatgagc tgcgatcagt gccacgagcg tgaagccgtc
     3301 attcatctga cgcagatcgt gaacgaacag gtcacgacgg tgcacttgtg cgagcgttgc
     3361 gccgcggaaa agggtgtcga gagcgctgca tcggtcgagc agaatccctt tgccaagctg
     3421 gtggccgcca gcatgggaaa gacaacgccc gatctcagtc tcgtaggatc gggcacgcgt
     3481 ggagcctgtc cgcgctgcgg cgccacgctg caggatttcc gggagagcgg gcgcctgggc
     3541 tgcgctgatt gctggcgggc gttcgaggcg ccgctcaggg atctgctccg aaggctgcac
     3601 ggctcgacgg tacacatggg cgagcggtac gccgatcggg tgggtgcacc ggtcacggcc
     3661 aatggggaca aggcccctcg tcaggctgac ctccgagagc agctccgatt ggcaatcgaa
     3721 accgagaact tcgagctg
//
LOCUS       DASRJQ010000511         2588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2914615, whole genome shotgun sequence.
ACCESSION   DASRJQ010000511 DASRJQ010000000
VERSION     DASRJQ010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2914615"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VFB89_11695"
     CDS             complement(<1..391)
                     /locus_tag="VFB89_11695"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HZM28015.1"
                     /translation="MTPKSSDFEEDLEPSTRGTSAATATGLVLAAAAAGLAVGLLVAP
                     DSGEQTRERLRQRMRSGLDDLSDGLGHRVRTLREEGTGAIRRLEKRLNKLEDRFDSAR
                     DAAIDAVEEPLERLRSEPRHNGAAPLLG"
     gene            complement(571..2073)
                     /gene="ahcY"
                     /locus_tag="VFB89_11700"
     CDS             complement(571..2073)
                     /gene="ahcY"
                     /locus_tag="VFB89_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HZM28016.1"
                     /translation="MTTAVDTGTRAGVERLPFKVKDLSLADWGRKEIRLAEHEMPGLM
                     ALRAEYGGEKPLKGARIMGSLHMTVQTAVLIETLVELGADVRWVSCNIFSTQDHAAAA
                     VVVGPDGTPDNPKGTPVFAWKGETLEEYWWCTEQALMWPDGGGPTLIVDDGGDATLLV
                     HRGADFEAAGKVPSFDASTEPEEWGVILDLLRAEQRRHPGRWSKVTAGLKGVSEETTT
                     GVHRLYEMMQAGTLRFPAVNVNDSVTKSKFDNLYGCRHSIVDGLNRATDVMLAGKVAV
                     ICGYGDVGKGCAQALKGQGARVVITEIDPICALQAALEGYQVTTLDEVVETGDIFITA
                     TGNKDIITVDHMRRMKDKAVVGNIGHFDNEIDMAGLKKGGIQRVNIKPQYDEFVFPDG
                     HSVLILAEGRLLNLGCATGHPSFVMSSSFTNQVLAQLDLHLSASGKPTISGTRYSEKR
                     VYTLPKKLDEKVARLHLPKLGVNLTQLSPEQAAYIGVPVEGPYKSDHYRY"
     gene            complement(2070..>2588)
                     /locus_tag="VFB89_11705"
     CDS             complement(2070..>2588)
                     /locus_tag="VFB89_11705"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZM28017.1"
                     /translation="APYVARVVAVDQSRAMLAAAKTRLRDYPNVDLRQGELESLPVAD
                     GELDAAILFLVLHYVAEPGRALAEAGRVLKPGGRLLVVDMTPHGRDEYRQTMGHLWQG
                     FSSEQMNEWLTGAGLGTGRYVTLPTDPRAKGPALFSLSASRALESALYGKDSTGHNST
                     MGSGTRKLRNRE"
BASE COUNT          422 a          878 c          786 g          502 t
ORIGIN      
        1 ggccgagcag cggagcggcg ccgttgtgcc tgggctcgct gcggagccgc tccagcggct
       61 cttcgactgc gtcgattgcg gcatcgcgcg ccgagtcgaa gcggtcctcg agtttgttca
      121 agcgcttttc cagccgtcgg atagcgccgg ttccctcctc ccgcaacgtg cgcacgcgat
      181 gtcctagccc gtcgctcaga tcatccaggc ccgagcgcat ccgctggcgg aggcgctcgc
      241 gggtttgctc gccggagtcc ggcgccacga ggagacctac cgccagtcca gcagccgcgg
      301 ctgccagcac caggccggtg gcggtggcgg cactcgtgcc cctggtcgag ggttcgaggt
      361 cctcttcaaa atcggaactt ttcggggtca tatatcctcc cgccgctggt ggcggcgctc
      421 aagatccgtc tgttgggcgg ctgttcacgc gcccattcct gaccacccca cagtaatgcg
      481 aggggccctg gccggtaaga gggttgagtc ggcgaatgcg agagggctcc ccctcactgc
      541 gggagccctc ggctcagcgg cggttggcga ctaataccga tagtggtccg acttgtaagg
      601 accttccacc ggcaccccga tgtacgcggc ctgctcgggg ctgagctgag tcaagttcac
      661 gcccagcttg ggcagatgga gccgggccac cttctcatcc agcttcttcg gaagggtata
      721 gacccgcttc tcgctgtacc gcgtccccga tatcgtcggc tttccgctgg cgctcaggtg
      781 cagatcgagc tgtgccaaca cttgattggt gaacgaggac gacatcacga agctcgggtg
      841 gcccgtggca caacccaggt tgagcaagcg gccctcggcc agaatcagga ccgagtgtcc
      901 gtctggaaac acgaactcgt cgtactgggg cttgatattc acccgctgaa ttccgccctt
      961 cttgagcccg gccatgtcga tctcgttgtc gaagtggccg atgttgccca ccacggcctt
     1021 gtccttcatc cgcctcatgt gatcgaccgt gatgatgtcc ttgttgccgg tggcggtaat
     1081 gaagatgtct cccgtctcga cgacctcatc cagggtcgtg acctgatagc cctccagcgc
     1141 cgcctgaaga gcgcagatcg gatcgatctc ggtgatcacc acgcgtgcgc cctggccttt
     1201 gagcgcctgc gcgcaccctt tgccgacatc gccataacca cagatcaccg cgaccttccc
     1261 ggcgagcatg acgtcggtgg ctcgattcaa gccgtccacg atcgaatgcc ggcaaccata
     1321 caggttgtcg aacttggact tggtcaccga atcgttcacg ttcaccgcag ggaagcgcag
     1381 ggtacccgcc tgcatcatct cgtaaagccg gtgcacgccc gtggtggtct cttcggatac
     1441 gcctttcaag ccggccgtga ccttgctcca gcggccggga tgacggcgct gctcggcccg
     1501 gagcaggtcc aggatcactc cccactcctc gggttcagtg gaggcatcga acgacggaac
     1561 cttgccggca gcttcgaagt cggcgccgcg atgaacgagc aacgtggcat ctccgccgtc
     1621 gtccacgatc agggtggggc cgccgccatc gggccacatg agcgcctgct cggtgcacca
     1681 ccagtactct tcgagcgttt cccccttcca tgcgaacacg ggcgtgccct tgggattgtc
     1741 aggggtgccg tcggggccca ccacgaccgc cgcggctgca tggtcctggg tcgagaagat
     1801 gttgcaggag acccaacgca cgtcggcgcc aagctcgacc agcgtctcga tcagcaccgc
     1861 agtctgaacc gtcatgtgca gggagcccat gatacgcgca cccttgagag gcttctcgcc
     1921 gccgtactct gcccgcaggg ccatgagccc cggcatttca tgctcggcca gccggatttc
     1981 cttgcggccc cagtcggcca gactgagatc cttcaccttg aacggcaggc gctcgacgcc
     2041 cgctcgggtt cctgtgtcaa ccgctgtcgt cattctcgat tcctcagttt tcgagtcccg
     2101 cttcccatcg tggaattgtg tcccgttgag tccttgccgt acagtgccga ctctagcgct
     2161 cgactcgccg acaaactgaa caatgccggt cctttggctc gcgggtcggt cggaagtgtc
     2221 acgtaacgcc cggttccgag gcccgctccg gtgagccact cgttcatctg ctcactcgag
     2281 aaaccctgcc ataggtgtcc catggtctgg cgatactcat cccgaccgtg cggtgtcatg
     2341 tcgaccacca acagtcggcc ccctggcttg agcacccgtc ccgcttcggc gagcgcccga
     2401 ccaggctcgg ccacgtagtg gaggacgagg aagaggatcg cggcgtccag ctcgccgtca
     2461 gccaccggca gggattccag ctccccttga cgcaggtcga cgttgggata gtctcgcagc
     2521 cgggtcttgg cggccgccag cattgctctc gactgatcga cggcaaccac gcgcgcgacg
     2581 tacggcgc
//
LOCUS       DASRJQ010000512        11586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3455913, whole genome shotgun sequence.
ACCESSION   DASRJQ010000512 DASRJQ010000000
VERSION     DASRJQ010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 11586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11586
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3455913"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..347
                     /locus_tag="VFB89_11710"
     CDS             69..347
                     /locus_tag="VFB89_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28018.1"
                     /translation="MWIVLLVLLVALMIPIVGIVIDSPIGRALARRLEGPEQVPPEIG
                     DLAKKIDLLEAEVDDLNRAVKSLEEESQFLHRLLESTSRSTLPPPDRP"
     gene            383..1927
                     /gene="murJ"
                     /locus_tag="VFB89_11715"
     CDS             383..1927
                     /gene="murJ"
                     /locus_tag="VFB89_11715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HZM28019.1"
                     /translation="MVAAGILLSRIVGLIRQRVLAHFLATSDAADAFSAAFRIPNLLQ
                     NLFGEGALSASFIPVYASLLAREDRKEAGRVAGAVGALLALVMALLVLVGVLATPLLI
                     DLIAPGFQGEKRLLAIRLVRILFPGAGLLVLSAWCLGILNSHRKFFLSYAAPVIWNVA
                     IIVALIVAGGRQGQYGLAVIAAWASVAGSALQVLVQLPTVLSVARGLRPSLDLASESV
                     RTVVRNFMPTFMSRGVVQISAYVDALLASLLPTGAVAALGYAQTLYTLPVSLFGMSVS
                     AAELPAMSSVTGNAAEIGAHLRTRLDHGLRQIAFFIIPSAMAFAALGQVLAAALFQSG
                     AFTRSDSFYVWGILAGAAVGLLAGTMGRLYASGFYALRDTRTPLRYAVLRVVLTTVLG
                     YLAAIPLPGLLGIPNRWGVAGLTASAGVAAWVEFALLRRALNRRIGATGLDARFTGSL
                     WVAAALAAAAGGGLALLFPRHQHPIALALAVIGVYGVVYFSVASALGVAEAGSYRRRL
                     NRLWGR"
     gene            1915..3765
                     /gene="uvrC"
                     /locus_tag="VFB89_11720"
     CDS             1915..3765
                     /gene="uvrC"
                     /locus_tag="VFB89_11720"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HZM28020.1"
                     /translation="MGQVEKSGVRGDDLGAKDALARKLETLPDGPGVYLWKDARGEVL
                     YVGKAKRLKSRVRSYIAADFATSPKNELLQRLIADVETIVVGREAESLLLENNLIKEY
                     RPRFNVRLKDDKSYPSIAVTLGEPFPRVLVTRRRNIEGAKYFGPYTDVAQLRRTLAII
                     RRIYTMRSCHDDLPREKRERPCLDYHIGKCRAPCVGWHSQEDYRAMVNDVVDFLEGRT
                     NDVRQKVREAMLAASDREDFERAARLRDALRWLERLEEPSSVELIGTGEADAIGFARD
                     GDDAVGVLIRVRGGRVISRDHTFLEGVEDETDEAILSAFLVRYYVPAENRVRRVVLPF
                     PPDDFDALREILPESDWTVPQRGTASRWRELADQNARHLLESLRIESFETEERAEDPV
                     YGLGRDLGLSVVPRSLVCVDISNNQGRDMVGSLVWFEAGRPKKSEYRKFKIKGVAQQD
                     DFAAIHEVITRYLTRRRDEQAPLPDLMVIDGGKGQLGAALDAAKQVGFDKLPIASLAK
                     REEEIFLPGRFESLRLPRRGSSLRLLQRARDEAHRFGLAYNRKQRTRRTITSELLEIP
                     GVGPTRRRRLLERFGSLAGVRSATAQELAAVPGFSSALAERILEHLSAKS"
     gene            3784..4683
                     /locus_tag="VFB89_11725"
     CDS             3784..4683
                     /locus_tag="VFB89_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HZM28021.1"
                     /translation="MPELPDVVVYLEALETRIVGKVLERIRLFSPFVLRSVDPPIAEA
                     SGRRVLGLRRIGKRLVWALEGDLFLVIHLMIAGRLRWRPAGAKPPGRIGLAAFDFPDG
                     TLILTEAGSKRRASINLLKGETALAQLDRGGLEPLDASGDAFRERLLSENHTLKRALT
                     DPRLFSGIGNAYSDEILHRARLSPLALTSKLDDEAIRRLYGAVREVLQDWTARLRAEA
                     EQKFPENVTAFREGMAVHGRFRKPCPLCGTPIQRIRYADNETNYCPTCQTGGRLLADR
                     GLSRLLREDWPRSLEEMEDRLTK"
     gene            4680..5909
                     /locus_tag="VFB89_11730"
     CDS             4680..5909
                     /locus_tag="VFB89_11730"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZM28022.1"
                     /translation="MTEVDQFSVPTERASAPMDWLLECSACERSSDPQGLPTVCPDCG
                     QPWLVCYPARRHPPEGRSLLGRRSTGEADTLSQGMWRYRRFLPLLPGEAPVSLGEGGT
                     PLLELDRSARDLGLGKVWLKDESTNPSGSFKARGLAMAMTRAVAGGAESFVIPTAGNA
                     GVALAVYAAKVGRPATVFAPTATPGTILAQIRAFGAELKLIDGHIGDCGRAARELSVR
                     TGAFDLSTLREPYRIEGKKTLGLELAEQFGWTLPDAIIYPAGGGTGLIGMWRAFRELQ
                     TAGWLHGQFPRLFAVQSSGCAPVVRAVEAGADRCEPWADPRTVASGLLVPNPLGGALM
                     LRAIRETSGGAVAVSDSDLEHEASTTSRREGVDLSPEGGATFAAARQLRSSGHLSSEH
                     RVILFNTGAGWLYRGGQ"
     gene            5906..6778
                     /locus_tag="VFB89_11735"
     CDS             5906..6778
                     /locus_tag="VFB89_11735"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZM28023.1"
                     /translation="MNDNSSGTQAKLQIDHVTLAGPDLAVIQTRFAELGLDAEYGGLH
                     SNGVTHMALLGFEDGSYIELISTRVPTTRSPLWNAQIQDNAGPAAWAVRSNDLFQERA
                     RLSRAGVAVRGPIFMTRDRPDGTALEWDLLFPGDAEPGATLPFAICDRTPRELRVSPS
                     PGAARSGIAGIAAVVLGVASIDATGRLFEQAYGLGDLTVTGQSGLGARIGRLADAPVY
                     LAEALPGASWLSNRIERYGDSPAAVLLGSSRNDQSRPVVPLLPAEDWFGQRVAWIDPA
                     KLFGWRVGLILPAA"
     gene            complement(6779..7483)
                     /locus_tag="VFB89_11740"
     CDS             complement(6779..7483)
                     /locus_tag="VFB89_11740"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28024.1"
                     /translation="MWEQAQQALNDSSDRVIQGLANLLPGIVALLVALALSAAAAWVL
                     ALLLRRFLRGIRFDERLEEWGLSGLAEWSPNKSPSLLVTRTVSWLIVLIGFLIGIAAF
                     DASITSEIAMRLFRYLPNVVAAVALFLLGNVFARFLARGVLISAVNHNVHYARLLSLG
                     VKWLVLILAAAMALEHLGIGGRIVELAFGILFGGIVLALALAVGLGSKDLVSRSLERQ
                     AEKPAAEEAPEPFRHV"
     gene            complement(7505..8737)
                     /locus_tag="VFB89_11745"
     CDS             complement(7505..8737)
                     /locus_tag="VFB89_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28025.1"
                     /translation="MADLETLSSELKDRLAQLGQAELVVAVPGVATGEALRDTVIRIR
                     SALNGVTPGPRTVLVHPDGAMTDPGSANGQPALLPFPITPVDRFPAPGESPSEAYRSV
                     FLASHRLGARACVVVGSDPTTISAEALQRLVQPVVEQDFDLITPCYLRRRFDALLSSG
                     VITPLSRSLYGKRLRFPVGADFSFSGRLVERQLQPMTDRGTMPIWFASDAACGGFKIG
                     QAQLDVPVPVQKDPPDVSSAVAQLVGPLFLDIERNAACWQKVRGSLPVPTFGMASAVA
                     EETGVVDVTRMVEAFRLGYRNLQDVWGVVLPPATLVELKRLTQLAPESFRLADDVWAR
                     IVYDFALAYRLRSMSRDHVLGAMTPLYRAWVASYATEVRTMGASAVEQRLERLGTAFE
                     AQKPYFVSRWRWPDRFNP"
     gene            complement(8762..>11586)
                     /gene="treS"
                     /locus_tag="VFB89_11750"
     CDS             complement(8762..>11586)
                     /gene="treS"
                     /locus_tag="VFB89_11750"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HZM28026.1"
                     /translation="YNQARIIFTDTEKSNWTWDPVAKAYYWHRFFSHQPDLNYDNPVV
                     VEEISRVLRYWLDMGVDGVRLDAIPYLVEREGTNCENLPETHTVIKAIRREMDQYANR
                     MILAEANQWPTDVRPYFGDGDECHMAFHFPLMPRIFMALRLEDRFPIVDIMAQTPPIP
                     AICQWALFLRNHDELTLEMVTADERDYMYFAYSADPRMRLNLGIRRRLSPLLDHNPRR
                     IELLNSLLLSFPGTPIIYYGDEIGMGDNIYLGDRNGVRTPMQWNADRNAGFSRATPGR
                     LYSPLIMDPIYGYEAVNVEAQQADPSSLLNWTRNMIALRKLFRVFGRGTLEFLHPTNR
                     KVLAYIRHYESEQVLCVANLSRFAQPVELDLSAYEGMMPVEMLGYVEFPAIKREPYPL
                     TLGPYGFLWFELHGDPQAIAARTSQTLEAETALDVSLGWSGLFDGSETGRLQNRVLPR
                     FLASQRWFGGKTRRITEVQVEDWGPISDSEAVLVVVRVAYDDADPDRYVLPLAMSFGA
                     AADAVLAAAPASVICAVIQGLQPGILHDALADDGTCISLLRLIEGEGQTATQHGQVRG
                     SRGACFDAARGSAAAALPPRRVAAEQSNTSVIYADRLILKLIRRYQSGINPEWELDRF
                     LTERAHFTHVPLYAGSLEYEHDGQAATLGILQGMVLNEGDGWTGALEELQRYYEASAP
                     KTFPLGSPDPREHVGMYLDAAATLGRRTAELHLALASASDDPALAPEPLDADDLGWIS
                     KDFHDRAARAFDRLKDNLPRLPDEAVEDAGLVLRRRREILARLRDIDHGVGGRRIRVH
                     GDYHLGQVLRVKGDYVILDFEGEPMKPLEERRVKHSPLRDVAGMLRSFSYAAYAALLG
                     YTARRPEEVERLEPWARLWERSTSIEFRRAYREGMAGSDLLPRTERGIDSLLETYLID
                     KALYELLYELDNRPTWVRIPLWGLLS"
BASE COUNT         2039 a         3707 c         3815 g         2025 t
ORIGIN      
        1 tggaggtcgc gcagacctgg tacagcgctg cccttgaggg ggctttcgag cccgcataga
       61 ctccgcgcat gtggatcgtc ctgctggtgc tgctggtggc gctgatgatc ccgattgtcg
      121 gcatcgtgat cgactctccg atcggccgcg cgctggcgcg ccggctggag ggaccggaac
      181 aggtcccacc agagatcggt gatctcgcca agaagatcga ccttctcgag gccgaggtgg
      241 atgacctcaa tcgcgcggtg aagagcctgg aggaagagag ccagttcctg caccgtctcc
      301 tcgagtcaac gtcccggtcc accctgccgc cgccggatcg gccctgagtc ccgatccggc
      361 gccatctcag cggccggcgg ccatggtggc cgcgggaatc ctgctgagcc ggattgtcgg
      421 gctgatccgc cagcgggtac tggcccattt ccttgcgacc tctgacgccg cggacgcctt
      481 ctccgcggcg ttccgcattc ccaacctgct ccagaacctg ttcggcgaag gcgcgctgtc
      541 agcgtcgttt atccccgtat atgcctccct gctcgcgcgg gaggatcgga aagaggcagg
      601 gcgggtagcc ggcgcggtgg gagcgctgct cgcgctcgtc atggccctac tcgtgctcgt
      661 gggtgtcctg gcaacgccgt tgctcatcga cctcatcgcg cccgggtttc agggagaaaa
      721 gcggctgctg gcgatccgac tggtgcgaat tctcttcccc ggtgccgggc tgctggttct
      781 gtccgcctgg tgcctcggaa ttctcaacag tcatcgcaag ttctttctct cgtacgccgc
      841 accggtgatc tggaacgtcg cgatcatcgt ggcactcatc gtcgcggggg ggcgccaggg
      901 acagtacggc ctggccgtga tcgcagcctg ggcttctgtg gcaggcagcg ctctccaggt
      961 cctcgttcag ctgccgacag tgctctccgt cgcacgggga ctgcggccct cgcttgacct
     1021 cgcgtccgag agcgtacgca ctgtggtccg caacttcatg ccgacgttca tgagccgggg
     1081 cgtagttcag atcagcgcct acgtggacgc cttgctcgcg agtctgctgc ccaccggggc
     1141 ggtcgctgcg ctgggttacg ctcagacgct ctataccctg cctgtgagcc tgttcgggat
     1201 gtcggtttcg gcggcggagc tgcccgcgat gtcgagtgtc accggaaatg ccgctgagat
     1261 tggggcccac ctccggaccc gactcgacca cggtctccgt cagatcgcct tcttcatcat
     1321 cccctcggcg atggcctttg ccgcactggg gcaggtgctg gccgccgcgc tgttccagtc
     1381 gggcgctttc acccgcagcg atagcttcta cgtgtggggg attctggccg gcgcggcggt
     1441 agggctcctg gccggcacga tgggccgtct ctacgcctcc gggttttacg cgctccgcga
     1501 tacccgcaca ccactccggt acgccgtgct tcgggtcgtg ctcacgaccg tactgggcta
     1561 tctcgcggcg attccgctgc ccgggctgtt aggcataccg aaccggtggg gcgtggctgg
     1621 cctcaccgcg tcggccggcg tggcggcctg ggtagagttt gcgctgctcc gtcgagcgct
     1681 caaccggcgg atcggcgcca ccggtctcga tgcccgcttc accggctccc tctgggtggc
     1741 cgccgctctg gcggcggcgg ccgggggagg gctcgcgctt ctgtttccgc gccaccagca
     1801 cccgatcgcc ctggccctgg ccgtcatcgg cgtgtacggg gtggtgtatt tttcagtggc
     1861 gtccgcgctg ggagtggcgg aggcaggatc ttatcgtcgg cggctcaatc gcttatgggg
     1921 caggtagaga aaagtggagt acgaggagac gacctgggag ccaaagacgc cctcgcgcgc
     1981 aagctcgaga cgctgccgga cgggccgggc gtatacctct ggaaggacgc acgcggcgag
     2041 gtgctgtacg tcgggaaggc caagcggctc aagtcccggg tacggagcta tatagcggcg
     2101 gacttcgcga ccagtcccaa gaatgagctg ctgcagcgcc tgatcgccga cgtcgaaacg
     2161 atcgtagtcg gccgcgaggc cgagtcgctg ctgctggaaa acaacctgat caaggagtac
     2221 cgcccgcggt tcaacgtccg gctcaaggac gacaagagtt acccttccat cgccgtgacg
     2281 ctcggcgagc cgtttccccg agtgctggtc acccgccgcc gaaacatcga aggcgccaag
     2341 tacttcggtc cttataccga cgtggctcag ctccggcgaa cactggccat catccgccgg
     2401 atttacacga tgcggagctg ccacgacgac cttccccggg aaaagcgcga acggccctgc
     2461 ctggattatc acatcggaaa atgccgcgcg ccctgcgtag gctggcacag ccaggaggac
     2521 taccgggcca tggtgaacga cgtggtggat ttcctggaag ggcgcaccaa cgacgtccgc
     2581 cagaaggtcc gtgaggcgat gctggccgcc agcgatcggg aagacttcga gcgagccgcg
     2641 cggcttcggg atgcgctccg ctggctggag cgactggaag agccgtccag cgtcgagctg
     2701 atcggcaccg gcgaggccga cgctatcggc ttcgcgcgcg acggcgacga cgcagtcggt
     2761 gtcctcatcc gcgtgcgcgg cggacgggtg atctcgcggg accacacgtt tctcgagggt
     2821 gtcgaggacg agaccgacga ggcgatcctc agcgcgtttc tggtccgata ctacgtgccg
     2881 gcggaaaatc gggtgcggcg agtggtactg ccctttccgc ccgacgactt tgatgctctg
     2941 cgcgagatcc tgcccgagag cgactggacc gtgccgcagc ggggaaccgc aagccgctgg
     3001 cgtgagctgg ccgaccagaa cgctcgccac ttgctcgaga gcctgcggat cgaatcattc
     3061 gagaccgagg aacgcgccga ggatccggtt tacggcctgg gtcgggatct cggcctcagc
     3121 gtggtcccga gaagcctcgt ctgcgtcgac atctcgaaca accagggacg ggacatggtc
     3181 ggctcgctgg tatggttcga ggccggacgt ccgaagaaga gcgagtaccg gaaattcaag
     3241 atcaagggcg tggcgcagca ggacgatttc gcagcaatcc acgaagtgat cacccgatac
     3301 ctgactcggc ggcgagacga gcaagcgccg ctgccagatc tcatggtgat cgacgggggc
     3361 aaggggcaac tcggcgctgc actggacgcg gcgaagcagg tgggtttcga caagctcccg
     3421 atcgcgagcc tggccaagcg ggaggaagag atctttcttc cgggccgctt tgaaagcttg
     3481 cggctgccgc gccgcggttc gtcgttgcgc ctgttgcaac gcgcgcgcga tgaagctcat
     3541 cggttcggcc tggcctacaa tcggaagcaa cgaaccaggc gaaccatcac gtccgagttg
     3601 ctcgagatcc cgggagtggg cccgacccgc cgccgccgct tgctggaacg ctttggaagc
     3661 ctggccggcg tccgatcagc gacggcccag gagctggcgg cagttcccgg gttttccagc
     3721 gccctcgccg agcgaatcct ggaacatctg agcgccaagt cgtgaccctc cacgctaccg
     3781 gccatgcctg agctgccgga tgtggtggtc tatctcgagg ccctggagac ccgaatcgtt
     3841 ggcaaggttc tcgagcgtat ccggctgttc agccccttcg tcctgcgcag cgtggatcct
     3901 cccattgccg aagcgtcggg tcggcgggtg ctggggcttc gacgaatcgg gaagcgtttg
     3961 gtctgggcgc tcgagggtga tctcttcctg gtcattcacc tgatgattgc gggccgcctc
     4021 cgctggcgac cggcgggtgc caagccgcct ggccgcatag ggctggctgc attcgatttt
     4081 cccgatggga cgctgatcct tacggaagcc ggcagcaagc ggcgcgcatc cattaatctg
     4141 ctcaaaggtg agacggcgct ggcccagctc gatcggggcg ggctcgagcc actggatgcc
     4201 tcgggcgacg cgtttcgtga gcggctgctg tccgagaatc acacgctgaa gcgcgcattg
     4261 accgatccac gattgttcag cggcatcgga aatgcctatt cggacgagat actgcatcgc
     4321 gcgcggctgt cgcctctggc gcttaccagc aagctcgacg acgaagcgat ccgccgactg
     4381 tacggagcag tgcgcgaggt tctccaggac tggactgcgc gactccgggc cgaggcagag
     4441 cagaaatttc ccgagaatgt caccgcgttt cgcgaaggca tggccgtcca cggccgcttc
     4501 cgcaaaccct gtcccttgtg cggcacaccg atacaacgca tccgctacgc agataacgag
     4561 acgaactatt gtcccacttg tcagacagga ggccgattgc tggccgatcg aggactgtcc
     4621 cggctgctgc gggaggactg gccccgaagc ctggaggaga tggaggaccg gctgaccaaa
     4681 tgaccgaagt cgatcagttc agtgtaccaa ccgagcgagc gtcggcccct atggactggc
     4741 tgctggaatg ctcggcctgc gagcgtagca gcgatccaca aggactcccc actgtctgcc
     4801 cggattgcgg gcagccctgg ctcgtctgct atcccgcccg acgccatcca ccggaaggcc
     4861 gctcgttgct cggccgacgc tcaacggggg aagccgacac tctgtcacag gggatgtggc
     4921 gctaccgacg cttcctgccg ctgttgcctg gtgaagctcc agtcagcctg ggagagggag
     4981 gcactccgct tctcgagctc gatcgcagcg cgcgagacct gggcctgggc aaagtctggc
     5041 tgaaagacga aagcaccaac ccgtccggct cgttcaaggc acgtgggctt gcaatggcga
     5101 tgacgcgtgc agtggcaggc ggagcagagt cgttcgtcat cccgaccgct ggaaatgccg
     5161 gcgttgcgct cgccgtgtac gcagccaagg tggggcgccc cgcgacggtg ttcgcaccta
     5221 ccgccacgcc cggcaccatc ctggcgcaga ttcgcgcgtt cggcgcggag ctcaaactga
     5281 tcgatggaca cattggggac tgcgggcggg cggcgcgtga gctttcggtc cgaacgggag
     5341 ccttcgactt gtcgacgctg cgtgaacctt accgcataga gggcaagaag acgctgggac
     5401 tcgagctggc ggagcaattc ggctggactc tgccggacgc gatcatctat ccggcggggg
     5461 gcggtaccgg tctgatcggg atgtggcgcg ccttccgaga gctgcaaacc gcaggctggc
     5521 tgcacggtca gttcccccga ttgttcgctg tacagtcgag cggctgcgcc ccggtagttc
     5581 gggctgtcga ggcaggggcg gatcgttgcg agccatgggc cgacccccga accgtagcca
     5641 gcgggcttct ggttcccaac ccactgggcg gcgccctcat gctgagggcg attcgggaaa
     5701 cgtccggcgg tgcagtggcc gtcagcgact ctgatctgga acacgaagcc agcacgacat
     5761 cccgccgcga gggcgtggat ctctcacccg aaggcggagc cacctttgca gccgcgcgac
     5821 aactgcggag cagcggccac ctgagttcgg agcatcgggt gatcctgttc aacaccggag
     5881 cgggctggct gtatcgcggc ggacagtgaa cgacaattca agcgggactc aagccaagct
     5941 ccagatcgat catgtgaccc tggccgggcc cgacctcgcc gtcatacaga ctcgtttcgc
     6001 cgagctgggc ctcgacgccg agtacggcgg tctgcattcg aacggggtca cccatatggc
     6061 cctgctcggc ttcgaggatg gctcctacat cgaattgatc tcgacacgag tgccgaccac
     6121 acgctcgcct ctctggaacg cccagattca ggacaatgcc ggcccggccg cgtgggccgt
     6181 gcggtcgaat gaccttttcc aggaacgagc acgactcagc cgggctggcg tcgctgttcg
     6241 cggcccgatc ttcatgacgc gcgatcgtcc ggacggcact gccctcgaat gggatctgct
     6301 ttttccggga gatgcggagc ccggcgccac gcttccgttc gccatctgcg atcgaactcc
     6361 gcgggagctc cgagtctcac catctcctgg cgcagccaga agcggcattg ctggaatcgc
     6421 ggccgtggtt cttggtgtcg catcgatcga tgctacgggc cggctattcg agcaggcgta
     6481 tggcttggga gatctcactg tgacgggcca atcagggctt ggtgcacgca tcgggcgctt
     6541 agctgatgcg ccggtctacc tggccgaggc cctaccgggc gcatcgtggc tgtccaaccg
     6601 cattgagcgc tacggggact cgccggcagc ggtattgctc ggctcgagcc ggaacgatca
     6661 gagcaggcct gtggttcctc tgcttcctgc cgaggactgg tttggccagc gggttgcgtg
     6721 gatcgacccg gccaagctgt tcggctggcg tgtcggcctg atcctcccag ccgcttgatc
     6781 agacgtgccg gaacggctct ggtgcctcct cggctgccgg tttctcagcc tggcgttcga
     6841 gcgaccggct caccagatcc ttggagccta gcccaaccgc cagcgccagc gccagcacaa
     6901 tgccgccgaa caatattccg aacgccagct ccacaatgcg gccgccgata cccagatgct
     6961 cgagcgccat cgcggccgcc agaatgagca ccagccattt gacaccgagg ctcagcaggc
     7021 gggcgtagtg cacgttgtga ttgaccgcgc tgatcagaac cccgcgagcc aggaaacggg
     7081 caaacacgtt tcccagaagg aacagcgcca ccgccgcaac cacattaggc aggtaacgga
     7141 acagccgcat cgcgatctct gaggtgatcg aggcgtcgaa tgcggcgatg ccgatcagaa
     7201 agccgatcag tacgatcagc cacgagacag tccgggtgac caacagggaa ggactcttgt
     7261 tgggcgacca ttccgcgagg cccgacagcc cccactcttc gaggcgctca tcgaaccgga
     7321 tgccgcgcag aaaacgccgc agcagcagcg ccagcaccca tgcggctgcc gccgacaggg
     7381 ccagggcgac taagagcgcg acgatcccgg gcagcagatt cgccaaccct tggataacac
     7441 ggtcggacga gtcattcaga gcttgctgag cttgctccca cattagcggt tcctccgctt
     7501 caccttacgg gttgaagcga tccggccatc gccaccggga cacgaagtag ggtttctggg
     7561 cttcgaatgc ggtgcccagc cgctcgagtc gttgctcgac ggccgacgcg cccatcgtac
     7621 gcacctcagt tgcgtaggac gcgacccagg cccgatagag cggcgtcatg gctcccagca
     7681 cgtgatcgcg actcatggag cgaagtcgat aggccagggc aaagtcgtag acaatgcgcg
     7741 cccacacatc atccgccagg cggaacgatt ctggcgcgag ctgggtgaga cgcttgagct
     7801 cgaccagtgt ggccgggggc agcaccacac cccacacgtc ctgcaggttc cgatatccca
     7861 accggaaggc ttcgaccatc cgggtcacat ccaccacgcc ggtctcctcc gcgacggcgc
     7921 ttgccattcc aaaagtgggc accggaagag agccgcggac cttctgccaa caggccgcgt
     7981 tccgctcgat atcgagaaag agagggccga ccagctgagc gacggcggag ctcacgtccg
     8041 gcgggtcttt ctgcaccggc accggcacgt ccagttgagc ctgtccgatc ttgaagccgc
     8101 cgcacgccgc atcgctggcg aaccagatcg gcatggtccc ccgatccgtc atcggctgca
     8161 gctgacgctc gaccagtcgc cctgagaaac tgaagtcggc tccgacagga aaccggagcc
     8221 gcttgccata cagagagcgg ctgaggggtg tgatgacccc gctgctcagc agggcatcga
     8281 agcggcggcg gagatagcac ggggtgatga gatcgaaatc ctgctccacg acgggctgga
     8341 ccaggcgctg gagcgcctcc gccgagatcg tcgtgggatc ggatcccacg acgacgcagg
     8401 ccctggcgcc cagccgatga ctggccagga acacgctgcg atacgcctcg ctcggactct
     8461 cccccggggc tggaaaacga tccaccggcg tgatgggaaa cgggagcagc gccggttggc
     8521 cgttggcgga gcccggatcg gtcatcgccc cgtcgggatg aaccagcacc gtcctgggtc
     8581 cgggggtgac gccgttgagt gcggaccgga tgcgtatcac ggtatcgcgc agcgcctcgc
     8641 ccgtcgctac gcccggcact gccaccacga gctccgcctg gcccagctga gccagccggt
     8701 ccttcagctc cgacgacagt gtctcgagat ccgccattcg aggctttcct aagacgcgga
     8761 gttacgagag caagccccag agagggatgc gaacccaggt gggccgattg tccagctcgt
     8821 acaagagctc gtacaacgcc ttatcgatca gatacgtctc cagcaacgaa tcgatgccgc
     8881 gttccgttct cggcaggaga tcgctgcctg ccatgccttc ccggtaggct cgccggaatt
     8941 ctatgctggt ggaccgctcc cacaggcgag cccacggctc gagccgctcc acctcttcgg
     9001 gacggcgggc agtatagccc agaagagcgg cataggcagc gtagctgaac gaccgaagca
     9061 tgccggcgac atcccggagg ggagagtgct tgaccctccg ttcctccagc ggtttcatcg
     9121 gctcaccctc gaaatccagg atgacgtagt cgcccttcac ccggagcacc tgccccagat
     9181 ggtagtcgcc gtgcacccga atccgccggc caccgacgcc gtggtcgatg tcgcgcagcc
     9241 gggccaggat ctcgcggcgg cgtcggagca ccaggcccgc gtcttctacg gcctcgtcgg
     9301 gaagtcgagg aaggttgtcc ttgaggcggt cgaacgcacg ggccgcgcgg tcgtgaaagt
     9361 ccttggagat ccagccgaga tcgtcggcat ccagaggttc gggcgcaaga gcggggtcgt
     9421 cgctcgccga cgcaagggcg aggtggagct cggcagtccg gcggcctaga gtggctgcgg
     9481 catcgagata catgcccaca tgctcacgag gatcgggcga gcccaggggg aaggtcttcg
     9541 gggcggacgc ttcgtaatag cgctggagct cttccagcgc accggtccag ccgtcgccct
     9601 cattcagcac catgccctgc aaaatgccca gtgtggccgc ctgaccatcg tgctcgtatt
     9661 cgagcgagcc ggcatagaga ggcacgtgag tgaagtgcgc tcgctcggtg aggaaccggt
     9721 ccagctccca ctccgggttg atccccgact ggtatcgccg gatgagcttc aggatgagtc
     9781 tgtcagcgta aatgactgag gtgttgctct gttcggcggc gacgcgccga ggtggcagcg
     9841 cagcggctgc cgatccgcgg gcggcatcga agcaggcacc tcgacttccc cgcacctggc
     9901 catgctgcgt cgcggtctgg ccctcgcctt cgatcagtcg gagcagggag atacaggtcc
     9961 catcgtcagc caaggcgtcg tggaggatcc ccggctggag tccctggatg actgcacaga
    10021 tcaccgaggc gggggcggca gcaagcacag catcggcggc ggcaccgaag ctcatggcca
    10081 gcgggagcac gtaacgatcg gggtcggcat cgtcgtaggc cacccggacg accaccagaa
    10141 ccgcctccga gtcgcttatc ggtccccagt cctcgacctg tacctccgtg atgcgtcggg
    10201 tcttcccccc aaaccagcgc tgactcgcca gaaatcgagg caatacccga ttctgcaagc
    10261 gcccggtctc ggagccgtcg aacagtccgc tccaacccag cgaaacatcc agtgcggtct
    10321 ccgcctcgag cgtctgggac gtgcgcgctg cgatcgcctg cgggtcgcca tgcagctcga
    10381 accagaggaa tccgtacggc ccaagcgtca gagggtaggg ctcccgtttg atggcgggaa
    10441 actcgacgta tcccagcatc tcgaccggca tcatcccttc gtaggccgag aggtccagct
    10501 cgaccggttg ggcaaagcgc gacaggttag cgacgcagag aacctgctcg ctctcatagt
    10561 ggcgaatgta cgccaggacc ttccgattcg tggggtggag aaactccagc gtcccgcgcc
    10621 caaagacccg aaacagcttc cgcagggcga tcatgttgcg ggtccaattg agcaacgagg
    10681 agggatctgc ctgctgcgcc tcgacgttga cggcttcgta gccgtagatc gggtccatga
    10741 tcagcgggct gtagagtcga cccggggtgg cccgcgagaa tcctgcgttg cggtcggcgt
    10801 tccactgcat cggcgtacga acgccgttgc ggtcgccgag gtagatgttg tcgcccattc
    10861 cgatctcgtc gccatagtag atgatcggcg tgcccgggaa tgagaggagc aggctgttga
    10921 gcagctcgat tcgacgaggg ttgtgatcga gcaggggaga caatcggcgc cgaatgccca
    10981 ggttgagccg catgcggggg tcggcgctat aggcgaagta catgtagtcc cgctcatccg
    11041 ccgtgaccat ctcgagggtg agctcgtcgt ggttgcggag gaagagagcc cattggcaaa
    11101 tcgccggaat gggtggggtc tgcgccatga tatcgacgat cggaaagcgg tcttccaggc
    11161 gaagggccat gaagatccgg ggcatgagcg ggaagtggaa tgccatgtgg cattcgtcgc
    11221 cgtcgccaaa atacgggcgg acgtcggtgg gccactggtt tgcctccgcg aggatcatgc
    11281 ggttggcgta ctggtccatc tcgcgccgga tcgctttgat gaccgtgtgg gtctcgggaa
    11341 ggttctcgca gttggtcccc tcgcgctcca ccagataggg gatggcatcg agtcgcactc
    11401 cgtccacacc catgtcgagc cagtaacgca gaaccctcga gatctcctcg acaacgaccg
    11461 gattgtcgta gttgaggtcg ggttgatgcg agaaaaaccg gtgccagtag tacgccttgg
    11521 cgaccgggtc ccaggtccag ttcgatttct cggtatcggt aaagatgatc cgcgcctggt
    11581 tgtatc
//
LOCUS       DASRJQ010000513         5121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2791300, whole genome shotgun sequence.
ACCESSION   DASRJQ010000513 DASRJQ010000000
VERSION     DASRJQ010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2791300"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..177)
                     /locus_tag="VFB89_11755"
     CDS             complement(<1..177)
                     /locus_tag="VFB89_11755"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZM28027.1"
                     /translation="MSKTRIALALGSGGLRGIAHIGVLRALAEAEIQPTVYAGSSAGA
                     LVAAAAAHDLSLSEM"
     gene            complement(259..861)
                     /locus_tag="VFB89_11760"
     CDS             complement(259..861)
                     /locus_tag="VFB89_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28028.1"
                     /translation="MARSIYKLVQLGGRRSESVFVITVMGFVLATGWLIHEVQAATAG
                     ELHEPVSDSAVRQPRVVSGVIESTTRTHRPAAAQPLPAGDAPAFLLASVQPLEAPASW
                     RRIYHEVEQCAGRRGDYDAIRWSVMEAPLQGPKGPTYAFTVGRRIVLVRGDTTYLRHE
                     MLHHILEVSGWHPRTLKADEHYTIADLHPMPLFGLCTGGH"
     gene            1175..1375
                     /locus_tag="VFB89_11765"
     CDS             1175..1375
                     /locus_tag="VFB89_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28029.1"
                     /translation="MSVLTLEQQARQKARIAMRLLADAQRLYEETGGAYDRPGLNAEA
                     LAQLVEELTHRIAEADRLAPLR"
     gene            1632..2861
                     /gene="hemA"
                     /locus_tag="VFB89_11770"
     CDS             1632..2861
                     /gene="hemA"
                     /locus_tag="VFB89_11770"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HZM28030.1"
                     /translation="MPSSPLHVFHAIGVSHRTAPLMVREQLVLTPAETAAWLDQQRAA
                     GRSTAILSTCNRFEVYWTGGYDIEPWFVELARDRSVEPATAVARWDGVACVRHLYQVA
                     AGLDSQILGETEILGQVRRAHQVAVEAGTNDREVDALFSGAIAAGRRIRRDTRLGKHP
                     ASVSSAAVDVALAAMTQRDSPTVVILGAGEVAAGVLRALADRRVGRVVLVNRRPERAA
                     ALAEKWAVESGPWAELSSLLPSADLLFVTTGAKHALLRSEELAAATARRESDLVVLDL
                     AVPRNVEPAARGQPRLRLFDLDDLQELCCPAAGHPSIAIAQAQQIIENELKRLEARLE
                     ARAAAPGLAQLHQLGQQLAREEADHALAGLDRLSDEERQVVRDLTQRVVRRVLYPVSR
                     SLREALSAHSEGARWTA"
     gene            2881..3276
                     /locus_tag="VFB89_11775"
     CDS             2881..3276
                     /locus_tag="VFB89_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28031.1"
                     /translation="MAGSPVETFSALIRAGRESGTKRVVGAATVRRLVLYYSAVVVLV
                     RLMELSLPLGPLGKTRMLAPWLERLAFSAVTGTCSVPEWVDAILAMAVAFMLTVPPVI
                     VYVTPGRTRRRTSRSSILSSFCHRWSRQF"
     gene            3258..3734
                     /locus_tag="VFB89_11780"
     CDS             3258..3734
                     /locus_tag="VFB89_11780"
                     /inference="COORDINATES: protein motif:HMM:NF027640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28032.1"
                     /translation="MVTAILIVVQSSLALAFSLAGIVAAVRFRSNVKDSRDALYILGG
                     VAIGFASGVYAVSVAVATSLLLVLLELFAWRSGWGGDRAYARAFLWGESPPAPAQGAT
                     RHGRQLFAQAGNNGRGKQPRGGLLRVETSDVDDGIRVVERVLGVTAKKWRFRCAYA"
     gene            4094..4441
                     /locus_tag="VFB89_11785"
     CDS             4094..4441
                     /locus_tag="VFB89_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28033.1"
                     /translation="MGPRGDRWQADGITSNAVNPGAVATGLQKHTGGLKTPMERRKTV
                     EQGAATSVLLAASPLLEGVSGRYFEDCNEAPLIARRPTDFSGGVAPFALDPANAERLW
                     DVSLKLIARRCLG"
     gene            complement(4433..>5121)
                     /locus_tag="VFB89_11790"
     CDS             complement(4433..>5121)
                     /locus_tag="VFB89_11790"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28034.1"
                     /translation="QLYRALVRLFGMDYVGARDLCESVLPVLGEPARTPWRRWALAIA
                     GSAEVGLGEHERALGHLMAAKQEMDDHTVINDWYCRMMVQSALTDLWLARGDLSRARQ
                     EAEVFLAVTSATAERTWQAWAWEANARLALRSSDLERAQDCITKALSVMEGYETPLAE
                     WRVHATATELSELRGDRVAAKHHREQSRGTVLRLANSLPEDDPLRKIFLSAPRISTIV
                     AGTELSGELS"
BASE COUNT          852 a         1661 c         1695 g          913 t
ORIGIN      
        1 catctcggag aggctcaggt cgtgggccgc ggccgctgcg accagcgcac ccgcgctcga
       61 tccggcgtag accgtcggct ggatctcggc ctcggcgagt gcccgcagga cgccgatgtg
      121 cgcgatgcct cgcaatcctc cgctccccag cgcgagcgcg atgcgcgtct tcgacatctt
      181 tccgaggttc cttctcgcaa gagttccgct tcgagcgtgc gcccgtcgat tcctcgacgg
      241 gaacgcgccc ttcggtgatc agtggccccc ggtacagagg ccgaagagtg gcatcgggtg
      301 caggtcggcg atggtgtagt gctcgtccgc cttgagcgtg cgaggatgcc aaccggagac
      361 ctccaggatg tggtgcagca tctcgtgccg gagataggtc gtgtcgcctc gcaccagcac
      421 gatccggcga cccacggtaa aggcgtaggt cggtcccttg gggccctgga gcggcgcctc
      481 catcacactc caccggatcg cgtcgtagtc gccccggcga ccggcgcact gctccacttc
      541 gtgatagatc cggcgccacg atgccggcgc ctcgagcggc tgcaccgagg ccaggagaaa
      601 ggcgggtgca tcacccgcgg ggagtggctg tgctgctgcc gggcggtgcg tgcgggtagt
      661 ggactcaatg acgcctgaca cgaccctggg ctgacgaacc gcggaatcgc tcacgggctc
      721 gtggagctcg ccggcggtcg ccgcctgcac ctcgtgaatc agccagccag tggccaggac
      781 gaagcccatg actgtgataa cgaagacgga ttccgagcgc cttccgccca gctggaccag
      841 cttgtagata ctccttgcca tacatcccct tggttcacca ccgttcgtgg tgtgccggga
      901 cccgcccccg gaccgaaggt ggggaaggca aagcgagtgc cagttggtaa acggttgatt
      961 cagctaggcg tcgactgcgg gattttgaaa cctcacgaag atgcaacgaa aatctcgcga
     1021 gggggccttc cgttttcgtg aggagattgg ctcgaggggc gcatcggttt cgtattgccc
     1081 tgcggagtaa tccttcggca caatagatgc ttccggtctg tcacgcggcg atttccgctg
     1141 atctcatccg ctgcgctcgg tccgggagga ttcgatgtcg gttctgacgc tcgaacagca
     1201 agcccgccaa aaggcgcgca tcgcgatgcg gctgttggcc gatgcccaac gcctctacga
     1261 agagaccggc ggggcctacg atcgtcctgg gctcaatgcc gaggcgcttg cgcagttggt
     1321 ggaggagcta acgcaccgga ttgcagaggc cgaccgtctg gccccgctcc ggtgaaccta
     1381 cagcaactcc cgcccacatc gtcgagtcga acatcaaatc cgtgaacata ggcaagcgcc
     1441 gcttcgcggc gatcccgcct tcgctcaatc gcgaagctcg ctcgcgagat tttcgcgaac
     1501 cctcgtgagg tttcacgagc tcctgtcgtc gtcctggtgg agctcaaggt tttaccagtg
     1561 gcactcggct tgccctgaca gcgaacacca tgctgtactc cggctcagcc ttcaccgact
     1621 gagggtgcaa gatgccatcc tctcccctgc acgtatttca tgccatcggg gtgagccatc
     1681 gtaccgctcc gctgatggtg agagaacagc tagttctcac gccggccgag acagcagcgt
     1741 ggttggatca gcagcgggcc gccggccgca gcaccgcaat tctctccacc tgcaaccgat
     1801 tcgaagttta ctggaccggc gggtacgaca tcgaaccctg gttcgtcgag cttgcacgcg
     1861 accggagtgt cgagccggcc acagcggtgg ctcgatggga cggcgtcgcc tgcgttcgcc
     1921 acctgtatca ggtcgcggcc ggactcgact cgcagattct gggtgaaacc gagattctgg
     1981 gacaggtgcg gcgggcgcac caagtagccg tggaggcagg cactaacgac cgagaggtcg
     2041 acgctctttt ctcgggcgcg attgcggccg gacggcgcat tcggcgggat accaggctgg
     2101 gtaaacatcc cgcgtcggtg agctcggcgg cggtggatgt tgccctcgct gcgatgaccc
     2161 agcgggactc ccctacagtc gtcatactcg gcgcgggcga ggtggcagct ggagtgctgc
     2221 gtgcgctggc ggatcggcga gtggggcggg tggtcctggt gaaccgccgc ccggagcgcg
     2281 ccgccgcgct ggcagagaag tgggcggtcg agtccggccc atgggccgag ctgagctcgc
     2341 tgctcccgag cgccgatctc ttgttcgtca ccaccggtgc gaagcatgca ctcttaaggt
     2401 ccgaagagct cgctgctgcc acggctcggc gcgagtccga cctggtagtg ctcgaccttg
     2461 ccgtgccacg gaacgtcgag cccgccgcgc gcggccaacc gaggcttcgc ctcttcgacc
     2521 tggacgacct ccaggaactc tgctgtccgg cagccggtca cccatccatc gccattgccc
     2581 aggctcagca gatcatcgag aacgagctca agcggctgga agcgaggctc gaggcacgtg
     2641 cggcggctcc agggctcgct caactgcacc agctcggaca gcagctcgcc cgagaggaag
     2701 cggaccacgc gctcgctggg ctcgaccgcc tgtccgacga ggagcgccag gtcgttcggg
     2761 atcttaccca acgggtggtg cggagggtgc tctatccggt cagccgtagt ctcagagaag
     2821 cgctgtccgc gcacagcgag ggtgcaaggt ggacagcctg agggcgtgga gtcggccctg
     2881 atggcgggct ctccggtgga gaccttttcc gccctcatcc gggcaggccg ggagagcggc
     2941 accaaacgcg tggtgggcgc cgccactgtg cgacgcctgg tgctgtacta ctccgcggtc
     3001 gtagtgctcg ttcgcctgat ggagctgagc ctgccgctcg gaccgttggg aaagaccagg
     3061 atgcttgcgc cgtggctgga gcggttggcc ttcagcgcgg ttaccgggac ctgcagcgtt
     3121 cccgagtggg tggacgcaat actggcgatg gccgttgcgt tcatgctcac ggtgccaccc
     3181 gtcatcgtct atgtgacacc cggacggacg aggcgtagga cgagtcgctc gtcaatactg
     3241 tcctcgttct gccatcggtg gtcacggcaa ttctgatcgt ggtacaaagc agcctcgccc
     3301 tcgcgttcag cctcgccggc atcgtcgccg ctgtccgctt ccgaagcaac gtcaaagact
     3361 cgcgcgatgc gctctatatc ctcggcggcg tcgctatcgg cttcgcctcc ggggtgtacg
     3421 cggtctccgt cgccgttgcg acctcgctcc tgttagtgct cctcgagctg ttcgcctggc
     3481 gcagcggttg ggggggcgac cgggcgtacg cgcgggcgtt tctctggggt gagagtcctc
     3541 ccgccccggc acaaggcgcc acgcggcacg ggaggcaact cttcgctcaa gccgggaaca
     3601 acgggcgcgg caagcagccc cgcggcggcc tgcttcgggt ggaaaccagc gacgtcgatg
     3661 atggaatccg ggtcgttgaa cgcgtgctcg gagtcacggc gaagaagtgg cgctttcgat
     3721 gcgcctacgc ctagcgcctg caaggcgatc gtcctggaat acgagtgccg actccgcttg
     3781 ccgtacaccc ccgacgcggt gcgaacccag ctgctgcacc agggggtgcc ttctgttcga
     3841 gcggcggaat gggagaggtg agaagcctga cgcgtaggac atcgtgcctt taataaccac
     3901 gccgtttggc ttccattcca ccgctgcgga cgtcatccgc ggtatcgacc tgcgaggaag
     3961 gcgggtcgtc atcaccggcg ctgcagccgg gaggggaatc cggggaagtg gcgaatctgc
     4021 taatcgaggg gaaccccgtc ctgccattcg gcgagacggg tcctacctct ttcgggtaag
     4081 cgctggagtg gcgatgggac cgcgagggga tcggtggcaa gccgatggca tcacatccaa
     4141 tgcagtgaac ccgggagccg tcgcgacggg actgcagaag cacacgggcg gcctgaagac
     4201 cccaatggag cggcgcaaga ccgtggagca gggggcggcg acgtctgtcc tactggccgc
     4261 ctcgccgctt ctggagggcg tcagcgggcg ctacttcgaa gactgcaacg aagcgccgct
     4321 gattgctcgt cgtcccaccg acttcagcgg cggtgttgct cccttcgccc tcgaccccgc
     4381 caatgccgag cggctctggg acgtctcgtt gaagctgatt gctaggaggt gcctaggata
     4441 gttcgcctga caactcggtt ccggcgacga ttgtcgagat gcgcggagcc gagagaaata
     4501 tcttgcgaag cgggtcatcc tccgggagcg agtttgcgag cctgaggact gtgccgcggc
     4561 tctgctcgcg atggtgtttc gccgccacgc ggtcgccacg tagttcggac agctcggtag
     4621 ccgtcgcatg cacccgccac tcggccaacg gggtctcgta gccttccatc accgacaacg
     4681 ccttcgtgat gcagtcctgc gcccgctcca ggtcgctcga cctgagtgcg agcctggcgt
     4741 tggcctccca tgcccacgct tgccaggtcc gctccgcggt cgcggacgtg accgcgagaa
     4801 agacttccgc ttcttgccga gcgcggctca ggtcgcctct ggccagccac aggtccgtga
     4861 gcgctgactg gaccatcata cgacagtacc aatcattgat gaccgtgtgg tcatccatct
     4921 cctgcttcgc cgccatcaag tggcccaaag cacgctcatg ctccccgagc cccacctctg
     4981 ccgatccggc gatagcaagg gcccagcgcc gccacggggt ccgggccggc tcgcccagca
     5041 ctgggagaac cgactcgcag aggtcccggg ctcccacgta gtccatcccg aagagtcgaa
     5101 ccaaggcccg gtagagctga a
//
LOCUS       DASRJQ010000514         7188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1009639, whole genome shotgun sequence.
ACCESSION   DASRJQ010000514 DASRJQ010000000
VERSION     DASRJQ010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7188
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009639"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(424..972)
                     /locus_tag="VFB89_11795"
     CDS             complement(424..972)
                     /locus_tag="VFB89_11795"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HZM28035.1"
                     /translation="MPVCIALLRAVNLPHSQVAMSDLRDLLLQVGFTEPRSLLQSGNL
                     VFRTRSTSTERLEALLESETRKRLDVDTDYMVRTAAEWEGLIAANPFPKEAQRDPGRL
                     LVVALKAKAEADRVGALQAAIQGPEIVRGVGKQLYVVYPNGVGRSRLTSSLIEKKLGT
                     RGTGRNWNTVLKLSTAAAELSR"
     gene            complement(997..1443)
                     /locus_tag="VFB89_11800"
     CDS             complement(997..1443)
                     /locus_tag="VFB89_11800"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZM28036.1"
                     /translation="MIYLLALLALVAGALIPVQAAANAALSKSLTGGVVASALTLFAV
                     AGTVALAAVAITRTPLPSWNEFRAAPVWSFVGGAIVAIYVLTITFLAPRIGVGTAIAF
                     IVTGQILAALTIDHFGLMRSLQVPINASRALGGVLMIVGAFLALRR"
     gene            complement(1447..1845)
                     /locus_tag="VFB89_11805"
     CDS             complement(1447..1845)
                     /locus_tag="VFB89_11805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZM28037.1"
                     /translation="MSSPLEFETRHLVLVVDDEPVVRRLAARVLDSAGYDVHEVGDGQ
                     QALDFLTARDTDVDVVVSDIVMPRLNGVQLLETLSRSHPDLPVILMSGYGPAELADRG
                     IAAPCALLAKPFLPEQLIAEVRRCIREDAA"
     gene            2297..2440
                     /locus_tag="VFB89_11810"
     CDS             2297..2440
                     /locus_tag="VFB89_11810"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZM28038.1"
                     /translation="MADDVKATWRELAGKGVQFLQEPKQADWGTAAIFTDPDGNQFVL
                     GSP"
     gene            complement(2447..2806)
                     /locus_tag="VFB89_11815"
     CDS             complement(2447..2806)
                     /locus_tag="VFB89_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28039.1"
                     /translation="MPSTQSRSASVTLAITVVIVLETIGLHLWLRQRHPVLAWALTGL
                     SLAALAWRNLPQAATEYLNAARPEDPNLLLTFPNADRPTHRCWTAPVRQLCLRVASPE
                     RAVEHWERWQAKSGTPA"
     gene            2917..3198
                     /locus_tag="VFB89_11820"
     CDS             2917..3198
                     /locus_tag="VFB89_11820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZM28040.1"
                     /translation="MSVQHDAANHRFVAQGPGGEAQLIYSYFADTVLNLEHTEVPPAA
                     RGHGVSDALIRAALAFARERNLNVMATCPFAQRWLAAHPNERPRPKASS"
     gene            complement(3211..3594)
                     /locus_tag="VFB89_11825"
     CDS             complement(3211..3594)
                     /locus_tag="VFB89_11825"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZM28041.1"
                     /translation="MITGAHFLLYSNDAAADRAFFRDNLGLPAVDAGGGWLIFGLPPA
                     ELGVHPAPAPFVQRHADHDIMGAVLYLMCDDLDSTVRSLASKGVRCTEVEMAEWGIKT
                     TMRLPSGGEIGLYQPTHPTALTART"
     gene            complement(3611..3862)
                     /locus_tag="VFB89_11830"
     CDS             complement(3611..3862)
                     /locus_tag="VFB89_11830"
                     /inference="COORDINATES: protein motif:HMM:NF021552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HZM28042.1"
                     /translation="MTEQHHEGLAMCRNIKKLRRPDEAPTDQELHDAALQFIRKISGF
                     NAPSKANEVAFNQAVQEVAAAGRELFASLHTHTTSQPTH"
     gene            complement(3855..4310)
                     /locus_tag="VFB89_11835"
     CDS             complement(3855..4310)
                     /locus_tag="VFB89_11835"
                     /inference="COORDINATES: protein motif:HMM:NF015004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HZM28043.1"
                     /translation="MQIWTIHPKYLDARGLVALWREALLARSVLRGYTTGYRRHPQLI
                     RFRSEPQPVACLDAYLRAIYQEAARRGYHFDRRKLGRATGPHRMMATSGQLAFEWRHL
                     KRKLRARSPAAYRAVRSESEPDPHPLFVVTPGPVSTWERTEERRRRQVD"
     gene            complement(4310..4822)
                     /locus_tag="VFB89_11840"
     CDS             complement(4310..4822)
                     /locus_tag="VFB89_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28044.1"
                     /translation="MSVLALRLIHIVCGVIWVGGVLFIAWFLIPTIRGVGPSSGAIMS
                     HMVQVRRLPLVMMAVAILTVLSGLALYWRDSAGFNGAWMQTGTGIVFSLGALLGLASV
                     AVGMAVNSPVSRRLGVLAAAAQAKEGPPSAEEAAEMQRLQTRLAAASNLVAALLVFAT
                     AAMAVARYVP"
     gene            complement(4863..5246)
                     /locus_tag="VFB89_11845"
     CDS             complement(4863..5246)
                     /locus_tag="VFB89_11845"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HZM28045.1"
                     /translation="MRFMLIAKATKDSEAGLPPDPRLMAAIGKLSEDMAKAGVLVDAG
                     GLYPSAKGARVRLSGGKVTVTDGPFAEARELIGGYAIVEAKSKAEAIELSERFWKLHA
                     ELMGPNYEGEGEIRQLHDPADFARA"
     gene            complement(5284..5718)
                     /locus_tag="VFB89_11850"
     CDS             complement(5284..5718)
                     /locus_tag="VFB89_11850"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HZM28046.1"
                     /translation="MRFMILRKADPNTEAGALPNQELLAAMGQYMEDMARAGILLAGE
                     GLRPTSEGVRVKFSRGKPTVTDGPFAEAKELIAGWSIIQVNSMEEAIEWVKRWPGIDG
                     DGEVELEIRRLYEADDFGAEFTPELGEKEERVRAQVAGSQQR"
     gene            complement(5865..7148)
                     /locus_tag="VFB89_11855"
     CDS             complement(5865..7148)
                     /locus_tag="VFB89_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZM28047.1"
                     /translation="MTTSDTHRAIDAVWRIESAKLIAGLARIVRDVGLAEDLAQDALV
                     AALERWPESGIPDKPGAWLMATAKHRAFDLLRRDKLLDRKHQELGHELEAKQETAAPD
                     LDTALDDPVGDDLLRLIFTACHPILSTEARVALTLRLIGGLTTEEIARAFLVPVATIA
                     QRIVRAKRTLGEAGVRFEVPRGEQLAARLSSVLGVIYLIFNEGYAATAGEDWTRPALC
                     EDALRLGRILAELASEEPEVHGLVSLMEIQASRLRARVGPTGEPILLLDQDRGRWDQL
                     LVRRGLAALERAEALGGVQGPYAVQAAIAACHASARSAAETDWARIAALYQNLGRISP
                     SPVVELNRAVAVAMAFGPAAGLEIADALIGERSLEQYHLLPAVRADFLIKLGRMDEAR
                     LELERAASLTQNARERKLLLERAAACSAEVASPQL"
BASE COUNT         1317 a         2334 c         2238 g         1299 t
ORIGIN      
        1 ggcggcggcg ctcttctccg gcgccggcat cgtcgcgacg cctaagggca cacctcgcca
       61 ccgtcaaatg ggccggatct atggcctgag catgctgggc ctcaacgtca ccgcgctgtt
      121 gatttgccgg ctgtccgcga cttttggccc cttccacgtc gctgcactct tgagcctgct
      181 cacggtcatc gccggcgttg ttgccgtcgt aagccggcgt ccgcaaggca cctggttgac
      241 cctgcatgcc aatttcatga gctggtccta tgtaggactg ctggccgccg cggctgccga
      301 agcccgcgac ccgaattccg gccgcaccgt tctggggaat ggtcgtcgta gcgagcctcg
      361 ccgtattcgc cgtcggaggc tatctcgtaa ttacgcggat tccgcgggtc ctgagcaacg
      421 ccttcagcgg gagagctctg ccgcggccgt gctcaacttg agcaccgtat tccagtttct
      481 tccggtacct cgagtcccca gcttcttctc gatcaggctg gaggtcaggc gcgaccgtcc
      541 gaccccgttg ggataaacca catacaactg cttacccaca cctcggacga tctccgggcc
      601 ttgaatcgca gcttgcaaag ccccgacccg atcggcctcc gccttcgcct tcagcgcaac
      661 cacgaggaga cggccggggt cgcgctgcgc ctccttgggg aacgggttcg cagcgataag
      721 cccctcccat tctgccgcgg ttctgaccat gtagtcggta tcgacgtcga ggcgcttcct
      781 ggtttccgac tcgagcagag cttcgagccg ctccgttgac gtggatcgag ttcgaaatac
      841 cagattgccg ctttgaagca gggagcgggg ttcggtaaag cctacctgaa gcaacaggtc
      901 gcgcaggtcg gacattgcga cctggctatg cggaagattg accgcgcgaa gcagcgcaat
      961 gcagacgggc atatccgaag tgggccaccc gctgcgctac cgccgcaggg cgagaaaagc
     1021 gccgacgatc atgagcacgc cacccaatgc acggctggca ttgatgggca cctgaaggga
     1081 ccgcatcaag ccgaaatggt cgatcgtgag cgctgccagg atctgtccgg ttacgatgaa
     1141 cgcgattgcc gttcccacac caatgcgtgg agcgagaaag gtgatggtga ggacgtaaat
     1201 ggccacgatc gcaccgccca cgaagctcca gaccggcgca gcccgaaatt cgttccacga
     1261 gggcaggggg gtgcgggtga tcgcgaccgc cgcaagcgcg acggttcccg cgacggcaaa
     1321 cagagtcagg gccgaagcga cgacgccgcc ggtcaacgac ttactgagcg ctgcgttagc
     1381 ggctgcctgt accggaatca gcgcccccgc cacaagggca agcagggcaa gtaaatagat
     1441 catcgcttac gccgcgtctt cacggatgca gcggcgcact tcggcaatca gttgctccgg
     1501 caggaacggc ttggcgagca aggcgcaggg cgccgcaatg ccgcggtcgg ccagctccgc
     1561 cggcccgtag cctgacatca ggatcacggg caggtccgga tgcgatctcg agagcgtctc
     1621 gagcagctgt acgccattca gccgtggcat gacgatgtct gagacgacca cgtccacgtc
     1681 tgtgtcgcgc gcggtcagaa agtccagcgc ctgctgaccg tcgccgacct catggacgtc
     1741 atagccggcc gagtccagga cgcgagctgc caatcggcga accaccggtt cgtcgtccac
     1801 taccagcacg aggtgcctgg tctcgaactc caaaggtgag ctcaaatgtc ctcctttatg
     1861 cggtggtggc gctcgaaacg cgatgcggac caccagcgcg cgatcaaaag tttatgggcg
     1921 cctatcaggg cccacttgaa cccgcatcac ttaacggagc caaggccgct tgatggcgta
     1981 aatagggatg ctggtcacgc tttcccgctg gatcgacacc aaacgacgtt tggccagaag
     2041 tcgtaaggcc cgtccgccca tcaggagact gtcatgatca ggccatcaag ttcatcggca
     2101 ttccagtgcg agaccaggag cgctcgcttc gattctacaa ggaaacgctc gggttcagag
     2161 tgatcaccga tcaacccttc gatgacaacc agcgttggat cgagctcggt atattgggcg
     2221 atcagcgacg ggtgttgctc tgtttacacc ggcgggtcat gaaaaccgca tcggcggact
     2281 taccaacatt tcgttcgtgg ccgacgacgt caaagcgacc tggcgcgagc tggcgggcaa
     2341 gggcgtccag tttctgcagg agcccaagca agcggactgg ggcaccgccg caattttcac
     2401 cgatcccgac ggcaatcagt tcgtgctcgg ctcaccctga cgagagtcac gccggagttc
     2461 ccgactttgc ttgccagcgt tcccagtgtt caacagcccg ttccgggctg gcgacccgga
     2521 ggcagagctg tcggaccggc gccgtccaac agcggtgtgt aggtcgatcg gcgttcggaa
     2581 acgtgagcag cagattgggg tcctccggcc tggcagcgtt cagatactcg gtagccgcct
     2641 ggggaaggtt ccgccaggcg agtgcggcca ggctcagccc ggtgagggcc cacgccagga
     2701 cgggatggcg ctgcctcagc cagagatgga gcccgatcgt ctccaagacg atgaccaccg
     2761 tgatcgcgag cgtgacgctg gctgaacggc tttgcgtcga cggcatccat tctgtattgg
     2821 catcagcact caccggaaat cctcgaaacc ttgccagcga gaaccccgaa gcggccgagc
     2881 gggttacatg agctgaccga accgattgga gtacgcgtgt ccgttcagca cgatgccgcg
     2941 aaccaccgct ttgtggctca gggcccgggc ggggaagccc aactgatcta cagctacttt
     3001 gccgacactg ttctcaacct cgagcacacc gaagtaccgc ctgcagcacg cggccacggt
     3061 gtttcggacg cgctcatccg cgcggcgttg gcgttcgccc gcgagcgcaa tttgaatgtg
     3121 atggcaacct gtcccttcgc tcaacggtgg ctggccgcgc acccgaacga gcgaccccga
     3181 cccaaagcgt cctcctgatt tcggcgcacc tcaggtgcgg gcagtgagtg cggtgggatg
     3241 ggtcggctgg tagagtccga tttcgcctcc gctgggcaag cgcatggtag tcttgatgcc
     3301 ccattcggcc atttcgacct cggtacatcg gactcctttg cttgccagcg atctcacggt
     3361 cgagtcgagg tcatcgcaca tcagataaag cacggcgccc atgatgtcgt gatcggcgtg
     3421 gcgctggacg aacggtgccg gggcggggtg aaccccaagc tccgccgggg gcaaaccgaa
     3481 gatgagccat cctccgcccg catctaccgc gggaaggccg aggttgtcgc ggaagaacgc
     3541 acgatctgcc gccgcgtcgt tgctgtagag gaggaagtgc gcgccggtga tcatcccgtt
     3601 ctcgagggcg ctagtgcgtt ggctgagatg ttgtgtgagt gtggagggag gcgaacagct
     3661 cccgaccggc cgccgcgacc tcctggacgg cttgattgaa cgcgacctcg ttggccttcg
     3721 acggcgcgtt gaagccgctg atcttgcgga tgaactgcag ggccgcgtcg tgcagctcct
     3781 gatcggtcgg ggcctcgtca ggccgtctta gtttcttgat gtttctgcac atagcaagtc
     3841 cctcgtggtg ctgttcagtc aacctgcctc cgcctgcgct cttccgtccg ctcccaggtg
     3901 ctgaccggac cgggcgtgac gacgaaaagc ggatgggggt cgggttcgct ctcagatcgg
     3961 actgcgcgat acgccgccgg actgcgagcg cgcaatttcc gcttgagatg ccgccattcg
     4021 aacgccagct ggccactcgt agccatcatc cggtgtggtc ccgttgctcg acccagcttg
     4081 cggcggtcga aatgataacc ccggcgagcc gcctcctggt aaatggccct gagataggcg
     4141 tcgaggcaag ctaccggttg cggctcggag cggaagcgga tcagctgggg atgccggcga
     4201 tatcctgtgg tgtatccgcg cagtaccgag cgtgccagca aggcctcgcg ccaaagggcg
     4261 acgaggccgc gggcatccag gtatttcggg tggatggtcc agatctgcat tacggaacgt
     4321 aacgcgccac cgccatcgct gcggttgcaa acaccagcaa tgcggctacc aggttggaag
     4381 ctgcagccag cctggtctgc agacgttgca tctcggccgc ttcctcagcg gaaggcggac
     4441 cttccttcgc ctgcgctgca gctgcgagca cgcccaggcg ccggctgacc ggagagttta
     4501 cggccattcc cacagccacc gatgcgagcc ccagcagagc gccaaggctg aaaacgatcc
     4561 ccgtccccgt ttgcatccag gcaccgttga agccggctga gtcacgccaa tagagcgcga
     4621 gtccggagag cacggtgagg atcgcgactg ccatcatgac cagtggcagt cggcgaacct
     4681 gaaccatgtg cgacatgatc gcaccacttg acggccccac gccgcgtatc gtggggatga
     4741 gaaaccaggc aatgaacagt acgccgccca cccagataac tccgcacacg atatggatga
     4801 gccgcaacgc caggacgctc atggtgtccg acctcccggg tacgcggtga agatagcggt
     4861 gctcatgcgc gagcgaagtc cgctgggtcg tggagctgac ggatctcgcc ctcgccctcg
     4921 tagttcggtc ccatcagttc ggcgtggagc ttccagaagc gctcggaaag ctcgatcgct
     4981 tcggccttcg acttggcctc cacgattgcg tagccaccaa tcaactccct cgcctcggca
     5041 aagggaccgt cggtaacagt gaccttcccg ccggaaagcc tgacgcgcgc acctttagcg
     5101 ctggggtaga ggccgccggc atccaccagc acgcccgctt tggccatgtc ctcggacagc
     5161 ttcccgatcg ctgccatgag tctcgggtct ggtggaaggc ccgcttcaga gtccttggtt
     5221 gctttggcaa tcagcatgaa ccgcattcct ggcatctcct tcagtcgagg gtaatggttc
     5281 tgatcagcgc tgctgcgaac ccgctacctg cgcgcggaca cgctcctctt tttccccgag
     5341 ctcgggcgta aactcggcac caaaatcatc tgcttcgtag agtcggcgga tctcgagctc
     5401 gacctctccg tcaccgtcga tcccgggcca acgcttcacc cattcgatcg cctcttccat
     5461 cgagttcacc tggataatgc tccagccggc gatcaattcc ttcgcctccg cgaatggccc
     5521 gtcagtcacg gtgggcttgc cccgggaaaa cttgacgcgc acgccttccg aggtcggacg
     5581 gaggccttcg ccggccagca gtatgccagc cctcgccatg tcttccatat actgtcccat
     5641 cgcggcgaga agctcctgat tcggaagtgc tcccgcttcg gtgttcgggt cggccttgcg
     5701 caggatcatg aatcgcatcg gtatgtctcc taacaggttt cgaaaatccg agccggggtc
     5761 ttggctccat caccggagcg aactcaggct cttacgtaca cgtcgaacaa acggtgtcga
     5821 aatcgacatt gggagcaaaa cggcaaacca ggagggtggc ggactcagag ttggggcgaa
     5881 gcaacctccg ctgagcaagc cgccgcgcgc tcgagcaaca gcttacgctc tcgggcgttt
     5941 tgggtgagcg aggccgcccg ctccagctcg aggcgggcct cgtccatgcg ccccagcttg
     6001 atcagaaaat ctgctcgaac ggcgggaagc agatgatact gctccagcga ccgctctcca
     6061 atgagtgcgt cggcgatctc gagaccagcg gccggcccga acgccatggc aaccgccacc
     6121 gcgcgattca gctccaccac cggcgacggg ctgatccgac ccaagttttg gtacagcgcc
     6181 gctatccggg cccaatcggt ttccgccgca ctccgcgcgc tggcatgaca ggccgcgatc
     6241 gcggcctgta cggcgtaggg gccttgcacg ccacccagtg cttctgcgcg ttcgagcgcc
     6301 gcgagcccgc gccgaaccag cagctggtcc caccgccctc gatcctgatc gagcagcagg
     6361 atgggttctc cggtcggccc gactctggcc cggagccgcg acgcctggat ttccatcagc
     6421 gacacgagac cgtgcacctc aggctcctca gaggccagct ccgccaggat gcggccgagc
     6481 cggagtgcgt cctcgcacag ggcgggtcgg gtccagtcct cgcccgcggt ggcggcgtag
     6541 ccctcgttga agatgaggta gatgacccca agcaccgacg acagtcgcgc ggccagctgt
     6601 tcgcccctcg gcacctcgaa tcgaacgcca gcctctccca gcgtgcgctt ggctcgcaca
     6661 attcgctgcg cgatggtggc gaccggcacc agaaaagcac gtgcgatctc ctcggtcgtt
     6721 aggccgccga tcaggcgcag cgtgagagcg actcgtgctt cagtggatag gatggggtga
     6781 caggccgtga agatcaggcg gagcagatca tcgccgactg ggtcgtcgag cgcggtatcg
     6841 aggtcggggg ccgcggtctc ctgctttgct tcgagctcgt gccctagctc ctggtgcttt
     6901 cgatcgagca gcttgtcgcg gcggagcagg tcgaacgctc gatgcttcgc ggtggccatc
     6961 agccaggcgc ccggtttgtc cgggatgccc gactcgggcc accgttcgag agctgcgacc
     7021 agcgcgtcct gcgcgaggtc ctcggccaag ccaacgtctc gcacgatgcg tgcgagcccg
     7081 gcgatcagct tggccgattc aatcctccag acagcgtcga ttgcgcggtg cgtgtccgac
     7141 gtggtcaccg cgaacgacta acgcaccccc gccggccggc gcaagcgc
//
LOCUS       DASRJQ010000515         3241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2414888, whole genome shotgun sequence.
ACCESSION   DASRJQ010000515 DASRJQ010000000
VERSION     DASRJQ010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2414888"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..922)
                     /locus_tag="VFB89_11860"
     CDS             complement(143..922)
                     /locus_tag="VFB89_11860"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HZM28048.1"
                     /translation="MTDVSISRLAETPLSGSVPRFEIPGWRDRFGVVAGITARGAVGS
                     EPFDLGLWTAESVGKVMTRWRAFRSAEAGFSGFAMAYQVHGSRVAWHERVNGWSILEG
                     LDGHLTSTPGLMLLVTVADCIPIYLIDRNGHAVGLLHAGWRGTAAGILGRGVEGLAEA
                     TGSSPADIIMHCGVGICGSCYEVGAEVMEACGLLSRPPGPWHLDLRALLTDQAVKLGV
                     GEVSVSSYCSAHDGASFFSHRRSGGSDGRMVAYIGLPVNAT"
     gene            complement(915..2198)
                     /gene="murA"
                     /locus_tag="VFB89_11865"
     CDS             complement(915..2198)
                     /gene="murA"
                     /locus_tag="VFB89_11865"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HZM28049.1"
                     /translation="MPLRFRVRGGRSLKGTVRPAGNKNAALPILAATLLAEGPVELSN
                     VPRIRDVETMVALLADLGATLEWTGPNALRLDTASARLKPLDIQLCARIRASILLAGP
                     LLARFGKVALPPPGGDVIGRRRVDTHFLALEQLGALVTVGDRYELEAKTLTGGDIFLD
                     EPSVTGTENALMAAVAARGRTTLRNAASEPHVQDLAKFLLTLGASIEGIGTNVYTIEG
                     GRPLHGAAYTIGPDHIEIGSFVGLAAVTNGQVTIDGVRSEDLRSTLLGFDRLGIRPRV
                     DGPRLIVDAEQERRVRPDLGGHVPKLEDGPWPAFPADLMSTTIVAATQCAGMLLVFEK
                     MFESRLFFVDKLISMGAQIVLCDPHRAVISGPARLKGGTVESPDIRAGMAMLLAALAA
                     DGVSTIYNVGQIERGYERIDERLRGLGAEIERLDD"
     gene            2292..2768
                     /locus_tag="VFB89_11870"
     CDS             2292..2768
                     /locus_tag="VFB89_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28050.1"
                     /translation="MLALLQTTGGPPWVAPTVALSLAVIALSFIAIAIAAVVVLARLV
                     KPLEELSKVIRGLEDDLTVAIRGVRQLTDQSQDLLSMVRQEAGAFVQTGRRLRRQVVK
                     GAERIRTRMADLESLYDVVHEEVEDTALDVAAVLRSVRRGNGVLGRIRRLLVPGRR"
     gene            2765..>3241
                     /locus_tag="VFB89_11875"
     CDS             2765..>3241
                     /locus_tag="VFB89_11875"
                     /inference="COORDINATES: protein motif:HMM:NF013078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HZM28051.1"
                     /translation="MSGRRLAAALLVVVVGILAFPGDAHAWTPGTHIYLGESVLANLR
                     LLPPSVADLLRAFPFDFLYGNIAADSSIAKKYAPVGRHCHFWHVGQEILDLAPSDGLR
                     AFGLGYLAHLAADTIAHNYFVPRQLMVTSSTASFGHSYWETRVELHLGDAYAKAAKD"
BASE COUNT          617 a         1041 c         1047 g          536 t
ORIGIN      
        1 gaaccatcgg ctcgcggaaa aaaaatgtgg gggaactccc ccacatcatc accaacggtc
       61 cgtgagaacc gtccgggaca gcacgccaaa agtactggtg agcagaccct tcgtcaatgc
      121 gacagccggg gcaaaccgcc cgttacgtcg cgtttaccgg aagaccaatg taggccacca
      181 tccggccgtc cgagcccccg cttcgccggt gactgaagaa gctggcccca tcgtgggcgg
      241 aacagtatga cgaaacactg acctcaccga ctccgagctt cactgcctgg tcagtcagca
      301 gtgcgcgaag gtcgagatgc cagggtcccg ggggccgaga gagaagtccg caggcttcca
      361 tgacctcagc acctacttca tagcagctgc cgcaaattcc tactccacaa tgcattatga
      421 tatcagccgg agacgatcca gtagcctcgg ccagcccctc gactccacgc ccgagtatcc
      481 cggctgcggt gcctcgccag cctgcatgca gcaagccgac agcgtggccg ttgcgatcaa
      541 tcaggtagat cgggatgcag tcggccacag taacgagcag catcaaccct ggtgtgcttg
      601 tcagatggcc atccagaccc tccaggatgc tccatccatt gacccgctcg tgccaggcga
      661 ctcgagagcc atgcacctgg tacgccatgg caaacccgct gaatccggct tccgccgacc
      721 ggaatgcgcg ccagcgggtc atgaccttgc ccaccgactc cgccgtccag agtccaaggt
      781 cgaaaggttc gctacctacc gctcctctgg ccgtgatgcc tgcgacaaca ccaaaccgat
      841 cgcgccagcc agggatttcg aaacgaggta cagaaccaga gagaggagtt tcagccagtc
      901 tcgaaatcga gacgtcagtc atccaagcgc tcgatttcag cgccgagccc gcgcagtcgc
      961 tcgtcgatcc gctcgtaccc ccgctcgatc tgccccacgt tgtaaatagt gcttacgccg
     1021 tccgcggcga gcgcggccag tagcatggcc atgccggctc gaatgtcggg cgactcgacc
     1081 gtcccgccct tgagccgggc cggaccggaa atgaccgctc ggtggggatc gcacagcacg
     1141 atctgcgcgc ccatgctgat gagcttgtcc acgaaaaaca gccgggactc gaacattttc
     1201 tcgaagacga gcagcatccc ggcgcactgg gtggcggcca cgatggtcgt ggacatgaga
     1261 tccgccggga acgccggcca ggggccatcc tccagcttcg gcacatgccc gccgaggtcc
     1321 gggcggactc gccgctcttg ctccgcgtcc acgatgagcc gcggtccatc cacccgcgga
     1381 cggataccca ggcgatcgaa gccgagcagc gtgctccgaa ggtcttctga gcgcaccccg
     1441 tcgatggtga cctggccgtt tgtgacggcc gccagtccca cgaagctgcc gatttcgata
     1501 tgatcggggc cgatggtgta cgctgcacca tgcaaggggc ggccaccctc gatggtgtac
     1561 acgttggttc cgatcccctc gatggatgcg ccgagcgtca acaggaactt ggccagatcc
     1621 tgcacgtgcg gctcgctggc agcgttgcgg agcgtagtcc ggccccgcgc ggcgaccgcg
     1681 gccatcaggg cgttctcggt tccggtgaca ctgggctcgt cgaggaatat gtcgccgccg
     1741 gtgagagtct tggcttcgag ctcgtagcga tcgcccacgg tcaccagcgc gccgagctgc
     1801 tcgagggcga gaaagtgggt gtcgacccgg cggcgtccga tgacgtcgcc acccggcgga
     1861 gggagcgcga ccttaccgaa gcgggcgagg agcgggccgg cgaggaggat ggacgcgcga
     1921 atgcgagcgc agagctggat atcgagcggc ttgagccgag cgctggcggt gtcgaggcgt
     1981 agtgcgttgg gaccggtcca ctcgagagtc gcgccgagat ccgcgagtag cgcgaccatc
     2041 gtctcgacat cgcgaattcg aggaacgttg gacagctcga ccgggccctc ggccagcagt
     2101 gtcgcggcga gaatcggcag ggcggcgttc ttattgcccg cgggacggac ggttcccttg
     2161 agggagcgac ccccacgaac tcgaaagcgt aaaggcatgg ccaaagctga gtcagacccc
     2221 gggaggcgtc aagcggatag gcgcattacc ttaccagtcc attacggggg aacgcatcgc
     2281 atatgcgcct gatgctcgcg ctattgcaga cgaccggcgg cccgccctgg gtcgcaccga
     2341 cggtcgcgct ctcgctggcg gtgatcgccc tgtcgttcat cgccatcgcg attgcagcgg
     2401 tggtggtgtt ggcgcgcctg gtgaagccgc tggaagagct aagcaaggta atacgcggat
     2461 tggaagacga tctcaccgtc gcgatcaggg gtgtgcgaca gctcaccgac cagagccagg
     2521 atctactgag tatggtacgg caggaagccg gtgcgttcgt gcaaaccggc cggcggctgc
     2581 ggcgacaggt cgtcaaaggc gccgagcgga tccggacaag gatggcggac ctggagagcc
     2641 tatacgatgt ggtgcacgaa gaagtcgaag acacggcgct ggacgtggca gcggtcctcc
     2701 ggtccgttcg gcgggggaac ggcgtgctgg gccggattcg gcggcttcta gtgcccgggc
     2761 ggcgatgagc ggacgacgcc tggccgcggc gctgctggtg gtagtggtcg gaatcctggc
     2821 gtttcccggc gacgcgcacg cctggacccc cggcacgcac atctatctgg gcgaatccgt
     2881 actggccaat ttgcggctcc tgcccccgtc ggtcgccgat ctcctccgag cattcccctt
     2941 cgacttcctg tacggcaaca tcgccgccga ctcatcgatc gcgaagaagt acgcaccagt
     3001 gggccggcat tgtcacttct ggcatgtggg tcaggagatc ctggacctgg cgccgagtga
     3061 cgggctgcgg gcattcgggc tgggatatct cgcgcacctc gcggccgaca ccattgcgca
     3121 caactatttt gtgcctcgac agctcatggt cacgagcagt accgcctcgt tcgggcattc
     3181 ctactgggaa acgcgggtgg agctgcacct gggcgacgca tacgcaaaag cggccaaaga
     3241 c
//
LOCUS       DASRJQ010000516         6013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3218649, whole genome shotgun sequence.
ACCESSION   DASRJQ010000516 DASRJQ010000000
VERSION     DASRJQ010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6013
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3218649"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /locus_tag="VFB89_11880"
     CDS             complement(<1..1058)
                     /locus_tag="VFB89_11880"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HZM28052.1"
                     /translation="MSHAFAFPARQSASRVTAILALASLVPLAAGRAQGDPTRLTLDR
                     LGRGEFEAQTYGPIRWTSGTTYTILEPSASSKDALDIVLYDAPSGKRSVLVSASRLVP
                     KGQSAPIDIEDFDWSPAGSQLLIYTNSKKIWRQNTRGDYWLADTATGSLRKLGSPDAK
                     PSTLMFATFSPDGRSVAYVREHNLYVEGVADGRITQLTTDGSPTVVNGTFDWVYEEEL
                     NLRNGFRWSPDGRRIAYWQLNMDSVRSFDLINDTDSLYSFVVPVQYPKAGTTNSAGRI
                     GVVSVTGGPTRWLDIQGDPRTQYLARMQWAASPREVVIERLNRLQNTNDVILADVETG
                     QSRTILTERDSAWTEVVD"
     gene            1145..2170
                     /gene="mtnA"
                     /locus_tag="VFB89_11885"
     CDS             1145..2170
                     /gene="mtnA"
                     /locus_tag="VFB89_11885"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HZM28053.1"
                     /translation="MPLPSPITWSADKRVRILDQTRLPEREEYRDLASIAEVAEAIRA
                     LRVRGAPLIGIAAAMGVAMEAMTNAEADLAGICDACRVLGAARPTAVNLRWAVDRMER
                     RAVQASAAGENLGEALLAEADAIHEEDRAMCARIGELGASLLSDDATVCTICNAGILA
                     TGGIGTALAPVYVAHQSGRRPRVIVPETRPLLQGSRLTAWELSRSGVSCTVIADGMLA
                     SRIRRGDVQCAIVGADRIAANGDVANKIGTYGLALAARAHGVPFYVAAPSSTVDLATP
                     DGSRIPIEERAREEVARVGERMVAPEGVPVWNPAFDVTPAELITGIITDRGVLAPSEL
                     AAATADT"
     gene            2189..3685
                     /gene="glpK"
                     /locus_tag="VFB89_11890"
     CDS             2189..3685
                     /gene="glpK"
                     /locus_tag="VFB89_11890"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HZM28054.1"
                     /translation="MSSILALDQGTTGSTALVIHEDGTVLGRGYREFTQHYPRPGWVE
                     HDPEEIFRITLDAAREALAGARERPAGLGITNQRETVVLWDRRTLTPVAPAIVWQDRR
                     TSDRCRELREAGLAPLLRDRTGLVPDPYFSATKLEWLLRDPGLRERAEKGELAAGTIE
                     SWLAAKLTGGRAHVSDHTNASRTLLYSLKARDWDPELLQIFHVPAGILPRVVPSAGAV
                     AECDASHLGAPLPIAGLAGDQQSALFGQGCVTPGKSKNTYGTGAFLLVHSGDARPLPA
                     EGVLATAACGPRGEPAYALEGSIFIAGAAVQWLRDGLGLIGTAAETEALARNLPDTGG
                     VHFVPAFVGLGTPHWEPEARGTITGITGGTTRAHLARAALEAMAFGTAELLVTMTGGA
                     QLSVPALRVDGGAAANDWLMQFQADVLGVPVERPDMVETTALGAAGLAGLALGVWRST
                     EDFLAGRRFARFEPKLDPAERARRLAAWDRAVRTALAWARHPRSVGDN"
     gene            3754..4176
                     /locus_tag="VFB89_11895"
     CDS             3754..4176
                     /locus_tag="VFB89_11895"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HZM28055.1"
                     /translation="MKAGHKFLLGAALIVGSVAFLITQGVRETGVYFLTPTELAQKSA
                     QDPTFVENVGFKVGAKVVPGSVHRDPAARSIDFEVSDGVKTYPVTYKGLVPDTFTDAN
                     DIEVVVEGRLGRDGVIRATDVLAKCGSRYEAQFGSKKA"
     gene            4183..>6013
                     /locus_tag="VFB89_11900"
     CDS             4183..>6013
                     /locus_tag="VFB89_11900"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HZM28056.1"
                     /translation="MTLLGNFSLWAAVLVGLWCAVIGLSGRWRDRPDLAATVTRSTYA
                     LFVCLAAASLALWRGLVSHDFNIEYVAAYTSRNLPPYYVVSAFWAGQKGSLLFWAVVL
                     SLFGTLAQALTPRRYADLLPYVAAVTSSVVVFFVSVMLFSANPFERLPFTPPDGRGLN
                     PQLQNVGMVIHPPMLYLGYISITVPFAFAAAALLSRKLDTGWIHAIRKWTLVSWLFLS
                     IGITLGMWWAYVELGWGGYWAWDPVENASLLPWLTMTAFLHSVMIQEKRRMLKRWNLG
                     LIVGTFLLSIFGTFITRSGVISSVHSFTQSNVGYFFLAFLVIAGTFGFTLLYTRWPLL
                     DAESQLDSVASREAAFLFNNLLLVGIAFSVLWGTLFPILSELVRGSKITVGPPFFNRV
                     NVPLGLLLLGLTGVGPLIAWRKASTANLKRQFRWPALAGLITLAILLAAGMRDLYPIT
                     ALALAGFVAGTVVQEFQRGARARMRMHQESLPLALTRLIVRNRRRYGGYIVHVGILIY
                     CVAFTGMAFKQEREATLKPGQSVQLRSPFGHTYTFTHLGVSQFESLNRIVTAGTVELR
                     KDGKPAGIITSEKRQHVDSFKRPTFEPSTEVGIRSGLQEDVYVV"
BASE COUNT          959 a         1907 c         1999 g         1148 t
ORIGIN      
        1 tcgtccacga cttcggtcca ggcgctgtcg cgctcggtca gaatcgtacg gctttggccc
       61 gtctcgacat cggcgagaat gacatcgttg gtgttctgca ggcggttgag ccgttcgatc
      121 accacttccc tggggctcgc cgcccattgc atgcgggcga ggtactgggt ccgcggatcg
      181 ccctgaatgt cgagccatcg ggtcgggccg ccggtcaccg aaacgacgcc gatcctgccg
      241 gcggagttgg tggtgcccgc cttggggtac tgaactggga cgacgaacga gtaaagggag
      301 tcggtgtcgt tgatgagatc gaagctgcgg acgctgtcca tgttgagctg ccaataggca
      361 atccgacgcc catcaggact ccagcgaaat ccgtttcgga gattcaattc ctcttcatac
      421 acccagtcga acgttccgtt caccacagtg ggtgacccgt cggtggtgag ctgggtgatg
      481 cggccgtcgg cgaccccttc tacgtagagg ttgtgttccc ggacgtaggc cacagaacgt
      541 ccgtcggggg agaacgtggc gaacatcagg gtagagggct tcgcgtcggg gcttcccagc
      601 tttcgcaacg aaccggtcgc cgtgtcggcc agccagtagt caccccgggt gttctgtcgc
      661 cagatcttct tcgagttcgt gtagatcaac agctgcgatc ccgccggtga ccagtcgaag
      721 tcctcgatgt cgatcggggc cgattggccc ttcgggacca gacggctggc cgagaccagc
      781 accgatcgct tgcccgaagg cgcgtcatag agcacgatat ccagcgcatc cttcgacgaa
      841 gccgacggct cgaggatcgt gtaggtcgtt ccgctggtcc aacgaatcgg accgtaggtt
      901 tgcgcctcga actctccacg acccaggcgg tcgagcgtca ggcgcgtggg atcgccctgc
      961 gcccgaccgg cggccagggg aacgagactc gccaaagcca ggattgctgt aacccgcgag
     1021 gcgctctgac gcgctgggaa agcgaaagcg tgggacatcg ggttctcgat cgattggacg
     1081 ggaggagagt aaggagtacg cggcaaaaaa acaggggcag ccggtcaggt atatcttccg
     1141 ccccatgccg ctcccatcgc ccattacgtg gtccgccgac aaacgggttc gcattctcga
     1201 ccagacccgc ctgccagagc gggaggagta ccgcgatctc gcgtccatcg ccgaggtggc
     1261 ggaagcgatt cgcgcgttgc gcgtgcgggg tgcgccgctt atcggaatcg ctgccgccat
     1321 gggcgtcgcc atggaagcga tgaccaatgc cgaggcggac ctggccggaa tttgcgatgc
     1381 ctgccgagtc ctcggtgcgg ctcgaccgac ggcggtgaac ctgcgctggg cggtggaccg
     1441 gatggaacga cgggccgttc aggcgtcggc tgcgggtgaa aatctgggag aggcgctgct
     1501 cgccgaagcc gatgcgatcc acgaggaaga tcgagccatg tgcgcccgca tcggcgaact
     1561 cggcgccagt ttgctgtctg atgacgccac ggtctgtacc atctgcaacg ccggaattct
     1621 ggcaaccggc ggcatcggca ccgcgctcgc accggtgtac gtggcccacc agtcgggtcg
     1681 tcggccccga gtgatcgtgc cggagacccg tcctttgctc cagggcagcc ggctgaccgc
     1741 gtgggagctg tcccgctccg gagtgagctg caccgtgatt gcggatggga tgctcgccag
     1801 ccggatccgc cgcggcgacg tgcaatgcgc catcgtgggg gccgatcgta tcgccgccaa
     1861 tggtgacgtc gccaacaaga tcggcactta cgggctcgct ctggccgcgc gggcccacgg
     1921 agttccgttt tacgtcgccg cgccgagctc gactgtcgat ctggcgacgc cggacggaag
     1981 ccgaattccc attgaggagc gggcccgcga agaagtggct cgtgtggggg agcggatggt
     2041 ggcgccggag ggagtgccgg tgtggaatcc ggcattcgat gtcacgccgg ccgagctgat
     2101 aaccgggatc atcaccgacc gcggcgtgct ggcgccgtcg gagctcgccg ccgccacggc
     2161 tgatacctga tagctgatag cactcctcat gtcttcgatc ttggcgctcg accaaggcac
     2221 caccgggtcc accgcgctcg tgattcacga ggacggcacc gttctcggcc gcggctatcg
     2281 cgagttcacc cagcactacc cccgacccgg ctgggtcgag cacgacccgg aggaaatctt
     2341 tcggatcacg ctcgatgctg cgcgggaggc actcgccggc gcgcgcgaga ggcctgcggg
     2401 tctcggcatc accaaccagc gcgagacggt ggtgctgtgg gaccggcgaa cgctgacgcc
     2461 ggttgccccg gcgatcgtct ggcaggatcg ccgtaccagc gaccgatgtc gcgagcttcg
     2521 ggaagccgga ctcgcgccgt tgctgcgcga tcggaccggg cttgtccccg acccgtattt
     2581 ctcggcgacc aagctggaat ggctgctccg cgatcctggg cttcgcgagc gagcggagaa
     2641 gggcgagctg gcggccggca cgatcgaatc gtggctcgct gccaagctta ccggcggacg
     2701 ggctcacgtc agcgaccaca ccaatgccag tcgaacgctc ttgtactccc tcaaggcgcg
     2761 cgattgggac cccgagctgc ttcagatctt ccacgttccc gccggcattc tgcctcgggt
     2821 ggtgccgtcg gccggggctg ttgccgagtg cgacgcgagc cacttgggcg cacccctccc
     2881 gatcgcaggt ctcgcaggcg atcagcagtc ggcgctgttc ggtcagggat gcgtcacgcc
     2941 aggcaagagc aagaacacct acggtaccgg cgcttttctc ctggtgcact cgggagacgc
     3001 aaggccgctc cctgcggaag gcgtactggc cacggcggcg tgcgggccgc gaggcgagcc
     3061 agcctacgcg ttggagggaa gcatcttcat cgccggcgcg gcggtgcagt ggctccggga
     3121 cgggctgggg ctcatcggca ctgcggcaga aactgaggcg ttggcgagga acctccccga
     3181 tactggtgga gttcattttg tgccagcgtt cgtgggtctg gggaccccgc attgggagcc
     3241 ggaggcgcgg gggaccatca cgggtattac tggcggcacc acccgagctc acttggcccg
     3301 ggcggcgctc gaggcgatgg cctttggtac cgcggagctg ctggtgacca tgaccggcgg
     3361 cgcccagctc tcggttccgg cgctccgggt tgatggtggc gccgcagcca acgactggct
     3421 catgcagttc caggcggatg tgctgggagt tccggtggag cgccccgaca tggtcgagac
     3481 gactgcactt ggggcggcag gtctggcagg cctcgcgctc ggtgtatggc gctccaccga
     3541 ggactttctg gcgggaaggc ggttcgcgcg tttcgagccg aagctcgacc cggcggagcg
     3601 ggctcgccgg ttggcggcgt gggatcgtgc agtccggacg gcgctcgcct gggcccgcca
     3661 cccccggagc gtgggagaca actgaaccgc gacgtcccgg ccgccgccgg cgtcggtata
     3721 ttgattactg atgttcgttc tgcgggagga gccgtgaaag ccggccacaa gttcctgctc
     3781 ggcgccgcgc tgatcgtcgg aagcgtcgcg ttcctcataa cccaaggcgt tcgcgagacc
     3841 ggcgtgtact ttctcacgcc gaccgagttg gcgcagaaga gcgcccagga tccgaccttc
     3901 gtcgagaacg tggggttcaa ggtcggggcc aaagtggtgc cgggctcggt tcaccgcgac
     3961 ccggcagctc gttccattga tttcgaggtg tccgacgggg tgaagacgta tcccgtgacc
     4021 tacaaaggcc tggtccccga cacgttcacc gacgccaatg acatcgaggt cgtggtcgaa
     4081 ggacggctcg gtcgagatgg cgtcatccgc gcgaccgacg tgctcgccaa gtgcggctcc
     4141 cgctacgagg cccagttcgg atccaagaaa gcctgagccg gcatgaccct gctgggcaac
     4201 ttttcgttat gggccgccgt cctggtcggg ctctggtgcg ccgtgatcgg actgtccggc
     4261 cgctggcgcg atcggcctga cctcgcggct accgtcaccc gctccaccta cgctctgttc
     4321 gtctgtttgg ccgcagcctc gctggcgcta tggcggggac tcgtctcaca cgacttcaac
     4381 atcgaatacg tcgctgcgta cacctcgcgg aacctgccgc cctactacgt agtctcggcg
     4441 ttttgggccg ggcagaaggg ttcactgctg ttctgggcgg tggtgctctc gcttttcggc
     4501 actctggccc aggccctgac ccctcgacgc tatgctgatc tgcttcctta tgtagccgcg
     4561 gtcacgtcgt cggtcgtggt gttcttcgtc agcgtgatgc tgttttccgc caatccgttc
     4621 gagcggcttc cctttacgcc tccggacggt cgcggactga atccacagct ccagaacgtc
     4681 ggcatggtga ttcacccgcc gatgctctat ctcggctaca tcagcatcac cgttccgttt
     4741 gccttcgctg ccgcggcgct gctctcccga aagctcgaca ccggttggat tcacgccatc
     4801 cgcaagtgga ccctcgtctc gtggcttttc ctgtcgatcg gcatcacgct cgggatgtgg
     4861 tgggcttacg tcgagctcgg ctggggcggc tactgggcgt gggatccggt cgagaacgcg
     4921 agccttttac cctggctcac catgacggcg ttcctccatt cggtgatgat ccaggaaaag
     4981 cggaggatgc tgaagcgatg gaacctgggt ctcatcgtgg gaacttttct gctgagcatc
     5041 ttcgggactt tcatcacacg ctcgggcgtg atctcgagtg tgcacagctt cacccagtcg
     5101 aacgtcggtt acttcttcct cgcgtttctc gtcatcgcgg gcacgttcgg ctttacgctg
     5161 ctctacaccc gctggccgct actcgacgcc gaatcgcagc tggactcggt ggctagccgg
     5221 gaagccgcgt tcctgttcaa taacctgctg ctggtaggga tcgcattctc cgtactgtgg
     5281 ggaaccctgt ttccgatcct ctccgagctg gtgcggggca gcaagatcac ggtcggtccg
     5341 cctttcttca atcgtgtcaa cgtcccgctg gggctgcttt tgctcgggct caccggtgtg
     5401 gggcccttga tcgcctggcg gaaggcttcg accgccaatc tgaagcgcca gttccgctgg
     5461 cctgcgctcg ccgggctcat taccctcgca atcctgctcg cggccggcat gcgcgacctt
     5521 tatccgatca cggcgctggc cctggccggg ttcgttgcgg gaaccgtggt gcaggagttt
     5581 caacgaggcg ctcgcgctcg gatgcggatg caccaggaat ctcttccgct cgcgctcacg
     5641 cgactgatcg tgcggaatcg gcgccggtat ggtgggtata tcgtgcacgt tggcatcctg
     5701 atctactgcg tcgccttcac cggcatggcg ttcaagcagg agcgggaagc taccctcaag
     5761 ccggggcagt cggtccagtt acggagcccg ttcggccata cctacacctt cacacacttg
     5821 ggcgtttccc agttcgaatc gcttaaccga atcgtgaccg ccgggaccgt cgagctccgg
     5881 aaagacggca agccggctgg gatcattact tcggaaaagc ggcagcacgt cgacagcttc
     5941 aagcggccca ccttcgagcc gtccactgag gtgggaattc ggagcgggtt gcaagaggac
     6001 gtctacgtcg tgc
//
LOCUS       DASRJQ010000517         3995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3457753, whole genome shotgun sequence.
ACCESSION   DASRJQ010000517 DASRJQ010000000
VERSION     DASRJQ010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3457753"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VFB89_11905"
     CDS             complement(<1..521)
                     /locus_tag="VFB89_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZM28057.1"
                     /translation="MSRRNTWSTLMANIHAARAGEGPLLPVPAIEFGLDTFGDVTVDA
                     DGKLLSQARVIRDVVTEAELADRVGVDFFGVGEHHRPDFAISAPEVVLAAIAGRTQRL
                     RLGTAVTVLSSDDPVRVYQRFATLDALSGGRAEVILGRGSFTESFPLFGYPLDDYEVL
                     FEEKLALFAAIRDA"
     gene            complement(623..1048)
                     /locus_tag="VFB89_11910"
     CDS             complement(623..1048)
                     /locus_tag="VFB89_11910"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HZM28058.1"
                     /translation="MHGFKGERTLMRIHIGERDRWHGRALYQAIVELLRKRHYAGATV
                     LRAIMGYGASSKLRTDRIEVLSLDLPIIIECVDTEERIAAILPELDEMIGGGLITLER
                     ARVIMYRPDLPVEDRTESWEIDRTGGRQSEPPPERHDPG"
     gene            complement(1094..1522)
                     /gene="crcB"
                     /locus_tag="VFB89_11915"
     CDS             complement(1094..1522)
                     /gene="crcB"
                     /locus_tag="VFB89_11915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HZM28059.1"
                     /translation="MPGHTLSEDSTLMIVWYVALGGAVGSVARFLFGEFLQQRSGLAF
                     PIGTLVINISGSLLLGFILRYTLATLAISPEVRALIATGFCGGYTTFSSFSYETAALL
                     EEGDLRRATLYIVLSVALSLVGTFAGLSLAGAVLNARRGF"
     regulatory      complement(1549..1619)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(1678..2424)
                     /gene="surE"
                     /locus_tag="VFB89_11920"
     CDS             complement(1678..2424)
                     /gene="surE"
                     /locus_tag="VFB89_11920"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HZM28060.1"
                     /translation="MRILIANDDGIYSPGLATLAKVAGQFGEVRIVAPDVEQSSMGHA
                     ITHSRPLSLRRTKLEEFDAWRVNGTPADCVALGASQWENVDVVLSGINLGANLGNSTW
                     HSGTLAAAKQAALLGLRGVALSTPVTEDQPDFKALVPWVTRVLELVLPQKELSLVNVN
                     FPPEPRAIRWTRQSVRHYDGQVVPGQDPRGRTHYWITVVPLGPPEEGTDRWAVEQGWV
                     SLTPLRLDLTDEKALAHAVSKAPPDRTADD"
     gene            complement(2576..3043)
                     /locus_tag="VFB89_11925"
     CDS             complement(2576..3043)
                     /locus_tag="VFB89_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28061.1"
                     /translation="MTSALRLSLALAASVATLAGCSSEAVAPSQPRVDAFIAGVTARG
                     GQVTAVLNDFVPPAASAGPIARVPATGTVSHAHGSRLRVGVTGAADFARVYVWSPGAT
                     GSWDILLPNDVTMEDLDITVSPALRAGNLRVRYTLEGPTGIGGPTEQLLQIGS"
     gene            3491..>3995
                     /locus_tag="VFB89_11930"
     CDS             3491..>3995
                     /locus_tag="VFB89_11930"
                     /inference="COORDINATES: protein motif:HMM:NF017508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor family protein"
                     /protein_id="HZM28062.1"
                     /translation="MSIVVEKTGEDAASKSLRVTVPLDRVREAEDRALKYYTTRARLP
                     GFRPGKAPVGVVRKRFGDAIRQSVLEEVIRESWETARQSESLKPIADPSIRNLKFEAD
                     SPIEFELLVEVRPEIKLEREGGFRLTRTVAPVADPTVEEQVQRLREDRSAWLPVENER
                     PVPGQMVR"
BASE COUNT          766 a         1308 c         1245 g          676 t
ORIGIN      
        1 gcatcgcgga tggcggcgaa gagcgcgagc ttctcctcga agaggacctc gtaatcgtcg
       61 agcgggtagc cgaagagcgg gaacgattcg gtgaacgagc cgcggccgag gatcacctcg
      121 gcccgtccgc ccgacaaggc gtccaacgta gcgaaccgct ggtacacccg caccgggtcg
      181 tccgagctga gcacagtgac cgccgtgccg aggcgcaacc gttgcgtgcg gccggcgatc
      241 gcggcgagta cgacctccgg tgcgctgatg gcgaagtcgg ggcggtggtg ctcgcccacc
      301 ccgaagaagt ccaccccgac ccgatcggcc aactcggcct cggtgaccac gtcacgaatc
      361 acccgggcct gcgacaacag cttgccgtcg gcgtccacag taacatcgcc gaaagtgtcc
      421 aacccgaact cgatggcagg gaccggtaac agtgggccct cgccggcccg cgcagcatga
      481 atgttcgcca tcagtgtgct ccaggtattg cgccgggaca tcgacgctgc tacgaggtca
      541 gccgctggcc ggccgggcca gcaagatgca acatgaatgt tataacatct atcgtcccga
      601 aaagtttccg ccccggccgg gcttagccag gatcgtgccg ctccggagga ggttcggatt
      661 gccggccgcc ggttcggtcg atctcccagc tctccgtgcg atcctcgacc ggtaggtccg
      721 gccgatacat gatcacccgc gcccgctcca gagtgatgag cccgccaccg atcatctcgt
      781 ccaactcggg aagtatcgcg gcaatgcgct cctctgtatc cacacactcg ataattatcg
      841 gcaagtcgag cgagagaacc tcgatccgat cggtgcggag cttggagctt gcgccgtaac
      901 ccatgatcgc gcgaagcacg gtcgcaccgg cataatgccg tttgcgcagc aactcgacaa
      961 tcgcctggta cagcgctcgc ccatgccagc gatcccgctc gccgatatgg atccgcatca
     1021 gggtgcgctc tcccttaaag ccgtgcatag cgttttctcc tcctcgagtc cttcggacgg
     1081 tcacgagccc ggcctagaac ccgcgacgcg cattgagcac agcaccggcg agactcagcc
     1141 ccgcgaatgt acccaccagc gatagggcga cgctcagaac gatgtaaaga gttgcccgac
     1201 gcaagtcacc ctcttctaaa agagcggcgg tctcatagct gaagctagag aaggtggtat
     1261 agcccccgca gaacccggtc gcgatgagcg ctcgcacttc gggcgagatc gccagtgttg
     1321 ccaacgtgta acgcagaatg aatcccaaca gcagcgagcc gctgatgttg atgaccaggg
     1381 tgccgatggg aaatgccagg ccagatctct gctggagaaa ttcgccaaac agaaaccgag
     1441 ccacgctgcc aaccgccccg ccaagggcca cataccagac gatcatgagg gtcgagtcct
     1501 ccgaaagagt gtgcccgggc atacaaaagc tcctgccggg ccgcttcggg ccttgcagga
     1561 gtcatcagcc gcttatggat cagcggcggt tcagggcgaa ctccatcgcc ggttggttgc
     1621 gacgggtagc ctaattgttt agtgcgggta cggtcaagtc accatcgctt gtctgcatca
     1681 atcatccgca gttcgatccg gcggtgcttt cgagaccgcg tgcgccagcg ccttctcgtc
     1741 ggtgagatcg agccggaggg gtgtgagcga gacccatccc tgctccaccg cccagcgatc
     1801 ggttccctct tcggggggcc ccagcggaac caccgtaatc cagtaatggg tccgcccccg
     1861 cggatcctgc ccgggaacaa cctgcccgtc atagtggcga accgactgtc gagtccatcg
     1921 aatggcgcgc ggctccggcg gaaagttcac gttgacgagc gacagctcct tctgcggaag
     1981 gaccagctcc agcactcgcg tcacccaggg gaccagtgcc ttgaaatcag gttggtcctc
     2041 cgtgaccggc gtgctcagcg ccaccccgcg caggccgagc aacgcggcct gcttggccgc
     2101 cgcaagcgtg ccggagtgcc aggtcgagtt gcccaggttg gcgcccaggt tgattcccga
     2161 cagcacgaca tccacgttct cccactgcga tgcgcccagc gcgacgcagt ccgccggcgt
     2221 gccgttcact cgccaggcgt cgaattcctc gagcttggta cgccgaagcg agagcggccg
     2281 ggaatgggtg atggcgtgtc ccattgacga ctgctcgacg tccggcgcga cgatccgcac
     2341 ctcaccgaac tgacccgcga ctttcgcgag cgtcgcgagc cccggactgt aaatgccatc
     2401 gtcattagcg atgagaattc gcatttggcc ctcgcctcgg gtaacacaca cctgtcacgc
     2461 ccggcgctgg ttgcctggca tactacgtac ctgtgtgtgt ggtcgtgggg aatgcggtga
     2521 gtacgctcac gtgccgcgca gcgtggcggc tgcgcggctc ggagattgga agcgatcagc
     2581 taccgatctg gaggagctgc tcggtcggtc caccgatgcc ggtgggacct tcgagcgtat
     2641 agcggactcg caggttgccg gcgcgaaggg cgggactcac cgtgatatcc aggtcttcca
     2701 ttgtgacgtc gttgggcaac aggatatccc aggatcccgt cgcgcccggc gaccatacgt
     2761 aaacccgggc aaagtcggca gccccggtaa cgccgacccg caaccggctt ccgtgtgcat
     2821 gagagaccgt gcccgtcgcg ggcacacggg caatcgggcc tgccgatgcc gcgggtggca
     2881 cgaagtcgtt gagcacagcg gtcacctgac cgccgcgggc ggtaacgccg gcgatgaagg
     2941 cgtccacgcg aggctgactg ggtgccacag cctcagaact gcatcccgcc agagtcgcaa
     3001 cactggcagc gagagcaagg ctcaaccgga gcgcgctggt catgggtacc tcgagccgag
     3061 ttgttcgtag gggagcgctg ccgatccgcg caacgagcgt accgcccatg caacccatag
     3121 tcagtcaccg gcccagcgga ctccgacttt ggcagctcaa gtgactacca ggtaaagcat
     3181 tagcgccgcc cagcgagcag cccgaatgcg gtcgcgaaaa cgctttcggt ttgcgggccc
     3241 gatatcgcct gtgcaggaca ggccgcggct tgcggcggag actgcggact accggtgtac
     3301 cctacctcac ctcgccagac ctgttcgggt cccccggatt ctcgatccgc cgtcccacct
     3361 cgctttctca tcgcgcgttg cccgtggctc taagcgatgg cgagcgccac aacgacctct
     3421 gaattcgtcg gcgacacgca ggccgacgca tctgccgatt gccctcgtaa cgggctagat
     3481 tctggtcctt atgagtatcg tcgtagagaa aactggcgag gatgccgctt ccaaatccct
     3541 tcgagtgacc gtcccgctgg atcgggtacg cgaggccgag gaccgcgccc tcaagtacta
     3601 caccacccga gctaggctgc ccggttttcg tcctggcaag gcgccggttg gcgtagtgcg
     3661 aaagcgattc ggcgacgcga tccgtcagtc agtgctcgag gaggtcatcc gggagagttg
     3721 ggaaaccgcg cgccagagcg aatccctcaa gccgatcgcc gatccatcca tccgtaatct
     3781 caagttcgaa gccgacagcc caatcgaatt cgagctgctg gttgaagtcc gtcccgagat
     3841 caagctcgag cgcgaaggag gtttccggct tacccgcaca gtggcacccg tggcggatcc
     3901 gacggtagag gagcaggtac agcgcctccg ggaagatcgc tcggcgtggc tgccggtgga
     3961 gaatgagcga ccggtgcccg gccagatggt ccggg
//
LOCUS       DASRJQ010000518         2284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_881086, whole genome shotgun sequence.
ACCESSION   DASRJQ010000518 DASRJQ010000000
VERSION     DASRJQ010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881086"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="VFB89_11935"
     CDS             complement(<1..947)
                     /locus_tag="VFB89_11935"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 family protein"
                     /protein_id="HZM28063.1"
                     /translation="MTTSPSRGQTRRWRQYLADERAEAAGYRQLAERRSGEEREILLA
                     LADAEKRHESHWLELLGDDAGPPRRSSLPTRLLVFLATHFGGLFVLALMQRAEGRSPY
                     ESDVDATDRMAADERIHGEVLRGLAARGRVRLSGTFRAAVFGANDGLVSNLALVLGIG
                     ATGVDVQTILFAGLAGLLAGALSMAAGEYVSVRSQRELLESTQPDPESQSALPHLDVD
                     ANELALVYRARGMEAPEAEEHARTVLATLHLEPDAPEAVGVDPHEAVGPAWQAAVSSF
                     CFFASGALVPVLPYLFGASGWAAVVVAAALVGMALLGTGA"
     gene            1353..>2284
                     /locus_tag="VFB89_11940"
     CDS             1353..>2284
                     /locus_tag="VFB89_11940"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HZM28064.1"
                     /translation="MGIREQATYRRAVEAVIWGMPAVNYQMMYDASTRAGGPGDSQIV
                     YWPGLLDWKNQTLTPSPDVIYVMPFFNTKEVGPLVLEVPPAGDEGALNGSIMNYWQVA
                     IEDIGPAGVDEGRGGKFVILPPDYDAELPEGYIPLQSDTYQGYGLIRSILRGGSDAEI
                     AQAIDYARKIKLYPLSQVDNPTETAWVDASTQRFDAAIPYDIRFFQALHKIIQAEPFL
                     HRDRVMIDLLRSLGIEAGKPFDPNSTSEQILAAAIDEARAWIDLEHEAVFHPFAEGAR
                     WAFPALPELVEGIETNFEQPDAYPVLARAVLYSFA"
BASE COUNT          428 a          799 c          701 g          356 t
ORIGIN      
        1 gcgccggtgc cgagcagcgc catcccgacc agtgcggcgg cgacaacgac cgcggcccac
       61 cccgaagcgc cgaacaggta cggcagcacc ggcaccaagg cgccggaggc gaaaaagcag
      121 aagctggaga ctgccgcctg ccaggcgggg ccgaccgcct catgcgggtc cactccgacc
      181 gcctccggcg catcaggttc caggtgcagc gtggccaaca ccgtacgcgc atgctcctcc
      241 gcctccggtg cctccatccc ccgagcccgg tagaccagcg cgagctcatt ggcgtccaca
      301 tccagatgcg gcaacgccga ctgcgactcc gggtcgggct gggtggactc cagcagctcc
      361 cgctgggacc gtaccgacac gtattcccca gcagccatgg acagcgctcc ggccagtagg
      421 ccagcaaggc cggcgaacaa gatcgtttgt acgtcgacac cggtggcgcc aatcccgagc
      481 accagggcca aattgctgac caggccatca ttggcgccga agacggccgc tcgaaacgtc
      541 ccggatagcc gtactcggcc ccgggcggcg agaccgcgca gcacttcgcc gtggatccgc
      601 tcatcggccg ccatcctgtc ggtggcatcc acgtcgctct cgtacggcga gcggccctcg
      661 gcgcgttgca tcaatgcgag cacgaaaagg cccccgaagt gtgtcgccag gaagaccagt
      721 agccgggtcg gcaggctgct gcggcgcggc gggccggcgt catcgccgag gagttccaac
      781 cagtgcgatt cgtgccgctt ctccgcgtcg gccagggcca gcagaatctc tcgctcctcg
      841 ccgctccggc gctccgccaa ctggcggtag ccagcggcct cagcgcgctc gtcggcgagg
      901 tactgccgcc accggcgggt ctggccacgg ctcggagaag tcgtcacacg cacatcatgg
      961 cggctgtcac agcctgatgg gctacgtcgt cttctacctg atcgtggcat tcgctgcggc
     1021 cgttcctgac gactggtcgg accggcagac tgaagcaaag cggaactatg accgccgcaa
     1081 cctccgcagc tgaattcgag cggcatcgcg atcatctgac cggagtcgcc taccgcatgc
     1141 tcgacggctg cccccaccgc cgtcgcggcc ttgatcatga aatccgtcgc acgcgccaca
     1201 cgcagtgagc cacaggcgcc catgcgccct gagcccgcga gcgcccatat gcgctgagcc
     1261 cgtcgaaggc gctcgacttc tacgcctgta gtggctgtgg accgcggtag ggcagcatta
     1321 cccgagtgag cacagattcc acatcgtcaa cgatgggcat tcgcgaacag gcgacgtacc
     1381 gtcgggcggt tgaagcggtg atctggggga tgcccgccgt caattaccag atgatgtacg
     1441 acgcgtcgac gcgagccggc ggcccagggg acagccagat cgtctactgg ccaggactgt
     1501 tggactggaa gaaccaaacg ctgacgccca gtcccgacgt gatctatgtg atgccgttct
     1561 ttaacaccaa ggaagtcgga cccctcgttc tcgaagtacc cccggccggc gacgaaggtg
     1621 cgctcaacgg cagcatcatg aactactggc aagtcgcgat cgaagacatc ggaccagccg
     1681 gcgtcgatga gggtcgcggc ggcaaattcg tgatcttgcc gccggactac gacgccgagc
     1741 tccccgaggg ctacatcccg ctgcagtcgg acacctacca gggctatgga ctcatccggt
     1801 caatcctccg cggcggcagc gacgccgaga tcgctcaagc catcgactat gcgcgcaaaa
     1861 tcaagttgta tccgctgtcg caggtcgaca atccaaccga aacggcgtgg gttgacgcga
     1921 gtacgcagag gttcgatgcg gccatcccgt acgacattcg ctttttccaa gcgctccaca
     1981 agatcatcca ggcggagccc ttcttgcatc gggacagagt catgatcgac ttgctccgat
     2041 cgctcggcat cgaggccggc aagccctttg atccgaactc cacctccgag cagatcctgg
     2101 ccgcagccat cgatgaagca cgggcctgga tcgacctcga gcacgaggcg gtcttccacc
     2161 cattcgccga gggcgcccgc tgggcatttc ctgcgctgcc cgaactggtc gagggcatcg
     2221 agaccaactt cgagcagccg gacgcctacc cggttctcgc ccgggccgtc ctctacagct
     2281 tcgc
//
LOCUS       DASRJQ010000519         3751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2020730, whole genome shotgun sequence.
ACCESSION   DASRJQ010000519 DASRJQ010000000
VERSION     DASRJQ010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2020730"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="VFB89_11945"
     CDS             complement(<1..1070)
                     /locus_tag="VFB89_11945"
                     /inference="COORDINATES: protein motif:HMM:NF036554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HZM28065.1"
                     /translation="MNGVVRVVWLGLGLALLGGSCRNVAAQAVPIRVELSGVSGDAER
                     NVRTVLQIARAADTGKVALERIRDLYGKATGDIQLALEPFGFYRSTVQPSLRKEGDTW
                     VARYAIQPGPAVLVRTVDVRVTGAGAKTPAFQKLVKDFPLHPGDTLLHLPYAAAKLGF
                     LTVATDSGYLDAKFDTADIKVDRAASTADVQLRFETGPRFEFGPVSFEQDVLDPRFLE
                     TRVSFKRGEPYRGDKLLDLQTALGQDSYFRRIEVIPRRDLAQGVQVPIEVQLEPRKPR
                     EYEVGAGYGTDTGPRGRFAVEWRRLNRRGHSAKAEITVSPIEQSISTEYRIPAVGSPT
                     GMLTFMAGYALLNPTVSNSKTIL"
     gene            1205..1627
                     /locus_tag="VFB89_11950"
     CDS             1205..1627
                     /locus_tag="VFB89_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28066.1"
                     /translation="MVSIGLKGVIVGGLVAGVVLNLIDYVLYGVVLARDFADAMQALG
                     KPPADSLVPLFVLLDFLYGIILIYLYASIRPRFGPGPKTAVWAGLLMWVLVAVLHAVG
                     EAPLGLLPMRLYLIGIAVSLIGIPLAAVAGARFYEEPA"
     gene            complement(1650..2090)
                     /locus_tag="VFB89_11955"
     CDS             complement(1650..2090)
                     /locus_tag="VFB89_11955"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HZM28067.1"
                     /translation="MSSPTTAVSPQALIEAAKAPLLAYNDKNWERVRATVTPNFVYDE
                     VATRRRLQNADDTIVVWKGWAQAFPDSQATFNETLVSGNTVVLDITWRGTHTGQLQTP
                     KGPVPGTGKPIEIRACIISEISGDKARSQRHYFDMLTLLEQLGL"
     gene            complement(2135..>3751)
                     /locus_tag="VFB89_11960"
     CDS             complement(2135..>3751)
                     /locus_tag="VFB89_11960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZM28068.1"
                     /translation="AQTLRDLTDLRRAEEELRRSREALDFALEAAALGTWDHDLITGE
                     LRWDSRAKALFGLAPDTPISHRRWADAVRTDDLVEAETARKKAVRDRTPFSIEYRILW
                     PDGSEHSIAMVGRATIDPATDQAVRMAGVMLDITERKRTEARLQEVLRLEAIGRLAGG
                     IAHDLNNMLVAILGFSDLLAGSFQPEDPRREDMEQITLAASRSATLTRQLLAFARREI
                     IQPRRIDVNGVVQRAERLLRPVLGESIELVFQLSPDVGAIFADPGQVEQVLMNLVLNA
                     RDAMPQGGRVLIETARVRLGGATSGQEVDSEATPGQYVMLAVSDTGHGMDPATLQRIW
                     EPFFTTKPSGRGTGLGLAAVYGAVKQSGGFVWAESAPGRGTVISVYWPEDQLKPEQLL
                     DSGGLPGAERGTETVLVVEDEPLVRSLTVRTLGRLGYRCFVAERAEHALRMVQDGEVR
                     PDLVITDVVLPGKSGGWLAEQLSLDLPGLPILFMSGFTDEEVMRRGLVAAGRPFLQKP
                     FAPGDLAREVRRVLDLGRSLVLPQLRAAGD"
BASE COUNT          652 a         1235 c         1177 g          687 t
ORIGIN      
        1 agaatggtct tgctgttgct gactgtgggg ttgaggaggg catagccggc catgaaggtg
       61 agcatgccgg tgggcgagcc gaccgcagga atgcggtatt ccgtcgaaat actctgttcg
      121 ataggagaca ctgtgatctc cgccttcgcg gagtggcctc ggcgattgag ccggcgccat
      181 tcgaccgcga agcggccgcg tgggccggtg tcggtcccgt accccgcgcc gacttcgtac
      241 tcgcgcggtt ttctgggctc caactgcacc tcgatcggga cctggacacc ctgagccaga
      301 tcccgtcgcg gaatcacttc gatgcggcga aagtaggaat cctggcccag tgccgtctgg
      361 aggtcgagca gcttatcgcc ccgatacggc tcgcccctct tgaacgagac ccgggtctcg
      421 agaaagcggg ggtcgagaac gtcctgctcg aacgagaccg gcccgaactc gaaccgcggg
      481 ccggtctcga accgaagctg cacgtccgcg gtggacgccg cccgatctac cttgatgtcc
      541 gcggtgtcga acttcgcgtc caggtagcca ctgtccgtgg cgacggtgag aaagccgagc
      601 ttggctgccg cataggggag atgaagcaag gtgtcgccgg gatgaagcgg aaaatccttg
      661 accagtttct gaaaagcggg cgtcttggct ccggcaccag tgactcgcac atctacggtg
      721 cgcacgagca cggcgggtcc cggctggatc gcgtagcgcg caacccaggt gtctccctcc
      781 tttcggagtg aaggctgcac cgtgctccgg tagaagccga agggctcgag cgcaagctgg
      841 atgtcgcctg tcgccttgcc atacagatcc cggatccgct cgagcgcaac cttcccggtg
      901 tcagcagccc gtgcgatctg aagcacagta cgaacgttcc gctcagcatc gccgctcacg
      961 cctgacagct ctacgcgaat cggcaccgcc tgtgcggcca cgtttcggca gctgccgcca
     1021 agcaaggcca gcccaaggcc caaccagaca acccgaacga ctccgttcac ggtgccgccg
     1081 aggaagccat agacctcaga gtctagggtc cggggtcacc gatcgctcgg tcgagggtca
     1141 catttgatta gcttcgagcc gcacaggcag gtcccgcacc cagtccttcc acagcgaggt
     1201 gcccatggtc tcgatcggcc tgaaaggcgt aatcgtcggt ggtctggtgg ccggggtggt
     1261 tctgaacctg atcgactacg tgttatacgg agtggtgttg gctcgggatt tcgcggacgc
     1321 catgcaagct ctcggcaaac cgcccgccga ctcgctggtc ccattgttcg ttttgctcga
     1381 cttcctctat ggcatcatcc tgatctacct gtatgcgtcc atccgcccgc gattcggtcc
     1441 gggcccgaag accgcggtct gggcgggctt gctcatgtgg gtgctggtgg cggtactgca
     1501 cgcggtaggc gaggcgccgc tgggcctact gccgatgcgg ctctatctca tcggcatcgc
     1561 ggtcagcctg attggaattc cgctcgcggc tgtggcgggc gctcgcttct acgaggagcc
     1621 cgcctgagcc acgccgcgga aaaggcggat tagagaccga gctgttcgag cagcgtcagc
     1681 atatcgaagt agtgccgctg gctccgagct ttgtcgcccg aaatttcgct gatgatgcag
     1741 gcgcgaatct cgatcggctt gccggtgccc gggaccgggc ccttgggggt ctgcagctgg
     1801 cccgtatggg tccctcgcca ggttatgtcc agcaccaccg tgttgccgct gaccagggtc
     1861 tcgttgaagg tcgcttggga atccggaaag gcctgggccc atcccttcca caccacgatc
     1921 gtgtcgtccg cgttctggag ccggcgccgg gttgcgacct catcgtacac gaaattgggc
     1981 gtgaccgtgg cacggacacg ctcccagttc ttgtcgttgt aagcgagaag tggcgccttt
     2041 gcggcttcga tcaaggcttg aggggagact gcggtcgtcg gagaggacac gacgcctcct
     2101 ttggctaaag tcggaaacga aggttggaaa acgattagtc gccggcagcc cggagttggg
     2161 gcaggaccag gctcctgccc aggtcgagta ctcgccggac ttcgcgcgcc agatcacccg
     2221 gcgcgaaggg tttttgcagg aacgggcggc cggccgcaac gaggccccgt cgcatgacct
     2281 cctcgtcggt gaagccggac atgaacaaaa tcggcaaccc ggggagatcg agggacagct
     2341 gctcggccag ccatccaccg ctcttcccag gcagcaccac gtcggtaatc accaggtcgg
     2401 gcctcacctc accgtcctgc accattcgca aggcgtgctc ggctcgctca gccacgaaac
     2461 aacggtagcc gagccgaccc agcgtccgca cagtgagcga gcgaaccagg ggctcgtcct
     2521 cgaccacgag gacggtttcg gtaccccgct ccgctcccgg taacccaccc gaatcgagca
     2581 gctgctccgg cttgagctga tcctcgggcc agtaaacgct gattaccgtc ccccgccccg
     2641 gcgcggactc ggcccagacg aaaccgccgc tttgcttgac agctccgtac accgcggcga
     2701 gcccgaggcc ggttccccgt cctgatggct tagtagtaaa gaacggctcc cagatccgct
     2761 gcaacgtggc ggggtccatt ccatggccgg tgtcgctcac cgccagcatg acgtactgcc
     2821 ccggtgtagc ttcggagtcg acctcctggc cggaggttgc acctccgagc ctgacccggg
     2881 ccgtctcgat cagtacccga ccaccttgcg gcatcgcgtc ccgcgcattg agcaccaggt
     2941 tcatcagcac ttgttcgact tgcccgggat cggcgaagat cgcacccacg tccggcgaca
     3001 attggaacac caactcgatg ctctcgccca ggactggccg caacagccgc tcagcccgct
     3061 gcaccacgcc gttgacgtcg atccgacgcg gctgaatgat ctccctgcgg gcgaacgcta
     3121 ggagttgacg agtgagtgtt gcggaacggg acgcagcgag cgtgatctgc tccatgtcct
     3181 cgcggcgggg atcttccggc tgaaagcttc cggccaacag gtcgctgaat ccgagaatcg
     3241 ccaccagcat gttgttcagg tcgtgggcga tcccaccggc cagccgaccg atcgcctcga
     3301 gccgaagcac ttcctgcaac cgcgcctcgg ttcgcttccg ttcggtgatg tcgagcatca
     3361 cgcccgccat gcgaacggcc tggtctgtag ccgggtctat ggtggcgcgc ccgaccattg
     3421 cgatcgagtg ctcgctccca tccggccaca ggatgcggta ttcgatggag aacggggtac
     3481 ggtcacgcac cgccttcttc cgagccgttt cagcctccac cagatcatcg gttcgcaccg
     3541 cgtcagccca gcgtcgatgg ctgatgggcg tgtcgggagc aagtccgaac aacgccttcg
     3601 ctctcgaatc ccagcgcagc tcaccggtga tgaggtcgtg atcccaggtt cccagggccg
     3661 cggcctcgag cgcgaagtcg agcgcctccc gactccgcct cagctcttcc tccgcgcgcc
     3721 gcaggtcggt gagatcacgc agtgtctgcg c
//
LOCUS       DASRJQ010000520         4632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1444554, whole genome shotgun sequence.
ACCESSION   DASRJQ010000520 DASRJQ010000000
VERSION     DASRJQ010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1444554"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VFB89_11965"
     CDS             <1..370
                     /locus_tag="VFB89_11965"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZM28069.1"
                     /translation="TAVVMGFILFVAGNAMAQSIRERTNELAVLKTLGFGDGKILAIV
                     LLESCAIAVIGGAIGLGLASLIIAQGDPTGGMLPIYYLPVRDLVFGIVLVLVLGLGTG
                     LLPAVQASRLKIVDALRRTA"
     gene            476..1663
                     /locus_tag="VFB89_11970"
     CDS             476..1663
                     /locus_tag="VFB89_11970"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZM28070.1"
                     /translation="MNWLSQTIAVTKVTLQSIPQRLGSSVVAIIGIGGVVIVFTAVLS
                     IAEGFRAAMQGTGDPRTVMVMRAGSDTEMTSGFSGEEARVITEAPGIERGADGVHASP
                     ELFVIIGHPLQRSGTDANVPLRGLTPIALRVRPDLHIAEGRMFTPGTSEIVVGRAASR
                     QFANLRVGSTVKSGKMTWPVVGIFEARGSVAESELWCDAKVLQSVYQRGNSYQSVYLR
                     LETVDSFQRLKDGLTTDPRLNVTVVREPDYYAQQSQVLQSVIWGIGLTIAVLMGLGAI
                     FGAVNTMYSAVATRTREIATLRALGFGSTPVVVSVLIEAVLLSLVGGVLGGLIAWAGF
                     DGYQTATMNLQSFSQVAFAFAVTPRLLMWGLMASTAMGLLGGLLPAYRAARVPVVSAL
                     RQL"
     gene            2078..2326
                     /locus_tag="VFB89_11975"
     CDS             2078..2326
                     /locus_tag="VFB89_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28071.1"
                     /translation="MRIDEVLQRIQGEFVEMPGLRLTPAQAQRLWGLERDTCDALLGA
                     LVDAKFLAQTRDGAFIRQEGATPTQVRNIRRGQTLAVA"
     gene            complement(2497..2961)
                     /locus_tag="VFB89_11980"
     CDS             complement(2497..2961)
                     /locus_tag="VFB89_11980"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZM28072.1"
                     /translation="MALNAGEDAPDFELRSHRGGTVRLSDYRGKKNVVLAFHPLAFTP
                     VCATQMTGYDTDKARLEQADAVVFGLSIDPQPSKAAWAKSLGSISFDLLSDFHPHGEV
                     AQKYGVFRPEEGFSERAIFIVGKDGRIKWSRVYDIPQQPDNAELLAALERLQ"
     gene            complement(2984..3478)
                     /locus_tag="VFB89_11985"
     CDS             complement(2984..3478)
                     /locus_tag="VFB89_11985"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZM28073.1"
                     /translation="MSRRVQLILAALVVAGLGLWWWKGGSSAEREVRALFEGFAREFN
                     SGTTDGIGLLARAAHIGESFTPDVVIELGHGSPPIQGRETLMGMASRLQPRTSAFVLE
                     FDDVNVEFGDPDHGEVTFTALFRRRSQGSGEESIDAREFAGEVVRSNGRWRVSKVVAI
                     DTLR"
     gene            complement(3479..3970)
                     /gene="lepB"
                     /locus_tag="VFB89_11990"
     CDS             complement(3479..3970)
                     /gene="lepB"
                     /locus_tag="VFB89_11990"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZM28074.1"
                     /translation="MSWLRRIALGRDPRRTLTRVAVLTIVSIITFGWLLTPVRVSGIS
                     MEPTYRDSRLNLVNRIAYRIRAPRRGDVVAIRLAGPNVLYVKRIVGLPSERVSIVDGT
                     VEINGQPLIEPYVQRRQRWNYPEVTVGPREFFVIGDNRGMRMVDHDFGRVDARRVLGM
                     LLF"
     gene            complement(4120..>4632)
                     /locus_tag="VFB89_11995"
     CDS             complement(4120..>4632)
                     /locus_tag="VFB89_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28075.1"
                     /translation="SIIYVPLAGGLVRPYERPRTGDLAGTVGSTAPSFVVSLPSENPS
                     MDLLKAPSPPTGVPVGTTGFIPTEPAAEPPAPAPEPVGTTGPVMLSPARTRIQTVQRP
                     VGLNTVFVDFQGARWFSAGPAIEYSPDRFTPIGEHLGFPVYQLKGEPGVVYLALLQGT
                     TGLVAPYKSR"
BASE COUNT          828 a         1524 c         1474 g          806 t
ORIGIN      
        1 caccgcggtg gtgatggggt tcatcctgtt cgtggccggg aatgccatgg cccagtcgat
       61 ccgcgagcgc accaacgagc tggcggtgct gaaaacgctc ggcttcggcg acggcaaaat
      121 cctcgcgatc gtgctgctcg agtcgtgtgc cattgccgtc atcggcggcg cgatcggtct
      181 cggcctggct tcgctgatca tcgcgcaggg tgatccgacc ggcggaatgc tgccgatcta
      241 ctacctccct gtccgcgacc tggtgttcgg catcgtcctc gtcctcgtgc tgggactcgg
      301 aaccgggctg ctgccggcgg tgcaggcgag ccggctgaag attgtggacg cgctgcggcg
      361 cacggcgtga gtggaaccag ttaccagttg ccagttgcca gttgagaaaa cggagctctt
      421 cctgggaact ggaaactggt aactggaaac tgatctagac cgtaaggact cctcaatgaa
      481 ctggctctca caaacgattg ccgtcaccaa ggtgacgctg cagtcgatcc ctcaacggct
      541 cggctcttcg gtcgtcgcga tcatcgggat cggcggcgtg gtcatcgtgt tcaccgccgt
      601 gctgtccatc gccgagggct ttcgggcggc gatgcagggc acgggggatc cgcgcacggt
      661 catggtgatg cgcgcgggca gcgataccga gatgaccagc ggattctccg gcgaagaagc
      721 gcgggtgatt accgaggccc ccggcatcga gcggggagct gatggcgtgc acgcctcacc
      781 ggagctgttc gtcatcatcg ggcacccgct gcagcgcagc ggcaccgacg cgaacgtccc
      841 cttgcgcggc ctgacgccga ttgcgcttcg cgttcgaccc gacctgcaca tcgccgaagg
      901 ccggatgttc accccgggca cgtccgaaat cgtggtcggc cgcgccgcct cccgccagtt
      961 cgccaacctg cgggtgggct cgaccgtcaa gtccggaaag atgacatggc ccgtggtcgg
     1021 gatcttcgag gcacgaggca gcgtcgccga gtcggagctg tggtgcgatg caaaggtgct
     1081 ccagagcgtc tatcagcgcg ggaactcgta tcagtcggtc tatctgcgcc ttgaaacagt
     1141 tgacagcttt caacgcctga aggacgggct cacgaccgat ccccggctga acgtgacggt
     1201 ggtgcgggag cccgactact acgcccagca gtcccaggtg ctgcagtcgg tgatctgggg
     1261 catcggcctc acgatcgccg tgctgatggg cctcggcgcg atattcggcg ccgtgaacac
     1321 gatgtattcc gcggtcgcga cgcggactcg ggaaatcgcg acgctccgag ccctgggttt
     1381 cggatccacg ccggtcgtgg tctcggtctt gatcgaggcc gtgctcctga gcctcgtcgg
     1441 cggcgtcctc gggggcctga tcgcctgggc cggattcgac ggctaccaga ccgcgacgat
     1501 gaaccttcag tcgttcagcc aggtcgcttt cgccttcgcc gtcacgccac ggctgctcat
     1561 gtggggcttg atggcttcga cggcgatggg tctgctcggg ggcctgctgc ctgcctatcg
     1621 agctgcgcgc gtgccggtcg tgtcggcgct gaggcagctc taagctgggg agcgggggaa
     1681 cgggagctgg ggagctggaa ggccggtggt aatcggaggc aaccagccac tcacatctgg
     1741 gcccagttgc ctctgatgag caccagcaat cgagctcccc ccgctccccc gctcccccgc
     1801 tcccgagctc cccagcttcc cccgccccgc agagctactg tatggaaacc agacgtcaga
     1861 ttttccgata catacgcttt ttcccagtgt tccctccgga cctccaacca gcacagccgc
     1921 actactgatc agataagaac cattacagca accatttatc acgattcatc aggcgaggat
     1981 acctgggtcg aaccactgaa aaatcaccag gtacgtccgt tgcacctgag tcgctattcg
     2041 gtaaagacca acggtcggcc aggaaggatg tgaacccatg cgcatcgatg aagtactcca
     2101 gcggatccaa ggggagttcg tcgagatgcc gggcctccgt ctgacccctg cacaggcaca
     2161 gcgtctgtgg ggactggagc gtgacacgtg tgacgctcta ttaggcgcgc tggtcgatgc
     2221 aaagttctta gcacagaccc gggatggtgc gttcatccgc caggagggcg ccacgccaac
     2281 ccaggtcagg aacatccggc gcggtcaaac gttggctgtc gcatagttac cccaccggtg
     2341 ccggccggct gactacctgc ctcgtcagcc aaacaggccg gctcacgacc cccgctcccg
     2401 ggcgattttg gcgcccgcgg ccaggttcgt cgaaggtaga gggctcaggc cctcaccttc
     2461 gacatcactc cttcggccag gccaaaaggc cggcccctac tggagtctct cgagcgcggc
     2521 gagcagttca gcgttatccg gttgctgcgg gatgtcgtac acccgcgacc acttaatccg
     2581 gccgtccttc ccgacgatga agatggcgcg ctcggaaaat ccttcctccg gccggaacac
     2641 cccgtatttc tgcgccactt cgccatgcgg atggaagtcg ctcagcaggt cgaacgagat
     2701 cgagccgagg ctcttcgccc aggccgcctt cgatggctgg gggtcgatgc tgagaccaaa
     2761 gacgactgcg tcggcctgct cgagccttgc cttgtccgtg tcgtagccgg tcatctgcgt
     2821 cgcgcagacc ggcgtgaacg ccagcggatg gaacgccagc accacgttct tcttcccgcg
     2881 atagtcggac agccgcaccg ttccgccacg gtggctgcgc aattcgaaat ccggggcgtc
     2941 ctcgcccgca ttcaatgcca tctctgactc ctgatcctcg aactcacctc aaagtatcga
     3001 tcgccacgac cttgctcacc cgccatcgcc cgtttgagcg gacgacttcc ccggcaaact
     3061 cgcgtgcgtc gatcgattcc tcgccggacc cctgactccg ccgccggaac agcgcggtga
     3121 aggtcacctc cccgtggtcc ggatccccga actccacgtt tacgtcgtcg aactccagca
     3181 cgaacgccga cgtgcgtggc tggagccggc tcgccatccc catcagcgtt tcgcgtccct
     3241 ggattggagg actgccgtgc cccagctcga tgacgacatc gggcgtgaag gactccccga
     3301 tgtgggccgc gcgcgcgagc agaccaatgc cgtcagtcgt gccggagttg aactcccggg
     3361 caaacccctc gaacagcgcg cggacttcgc gctcggcaga cgagccgcct ttccaccacc
     3421 agaggccgag gccggcaacc accagggcgg caagaatgag ctggacgcgt cgcgacattc
     3481 agaacagcag cataccgaga acgcgccggg catccacgcg tccgaaatca tgatcgacca
     3541 tgcgcatgcc gcggttgtcg ccgatgacga agaattcgcg gggacctacg gtcacttccg
     3601 gatagttcca tcgctggcgc cgctggacgt acggttcgat caggggctgg ccgttgattt
     3661 cgacggtgcc gtcgacgatc gagacccgtt cggacgggag tccgacgatc cgcttgacat
     3721 acagcacgtt cggccccgcg agacgaatcg ccaccacatc gccgcgccgc ggtgcacgaa
     3781 ttcgatacgc tatccggttc accagattca gcctggaatc gcggtaggtg ggctccatgc
     3841 tgatgccgga cacgcgaacc ggcgtcagca gccacccgaa ggtgatgatg ctgacgatcg
     3901 tgagcacggc aacccgcgtc agagtccgcc gagggtcacg gcccagggcg atgcggcgaa
     3961 gccaggacac ggaacgcaag tatatcgatc gcgggttcgc gggttcgcgg ctatgggcag
     4021 atcgaacaat ggggtggaag gcgggccaac aggctagttg gcggccttgc accccattat
     4081 tcgaagcgtt tcgaacccgc gagcccgcga acccgcgacc taccggctct tatacggtgc
     4141 caccagcccc gtcgtcccct gcagcagcgc gaggtagacc acccccggct cccctttcag
     4201 ctggtacacg gggaagccga gatgctcgcc gatcggggtg aagcgatcgg gcgagtactc
     4261 gatcgccggg cctgcggaga accagcgcgc gccctgaaag tcgacgaaca cggtgttcag
     4321 gccgacgggc cgctggacgg tctggatccg ggtccgcgcg ggagacagca tgaccggacc
     4381 cgtggtcccg actggctcag gcgcgggggc gggcggctcg gcagcgggct ccgtcgggat
     4441 gaagccggtg gtgccaaccg gcacaccggt tggcggagac ggcgccttca gcaggtccat
     4501 cgacggattc tcagatggca acgacacgac gaatgatggc gcggtgctgc cgaccgtccc
     4561 ggccaggtcg ccagtccggg gacgctcata gggccgcacc agcccgccgg caagcggcac
     4621 atagatgatg ct
//
LOCUS       DASRJQ010000521         3852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3619794, whole genome shotgun sequence.
ACCESSION   DASRJQ010000521 DASRJQ010000000
VERSION     DASRJQ010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3619794"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VFB89_12000"
     CDS             complement(<1..746)
                     /locus_tag="VFB89_12000"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HZM28076.1"
                     /translation="MDHAMYKKKWLVMGLSAAVVVGLAAAAGKDEQPLKGAIIYDYDP
                     ATKVGDAVFGTLIADMEHDHPGRCSHGGPVAMEYANGDLVAFYANTSDHNLDGWSEYA
                     VSKDKGKTWARYNKFKYSYAAYQADPKRPVWVEEGLVTRRGTVILVLTRFDNNQRTGN
                     GVMRSADHGATWTDYAPLDGDFVGYPCSVAVAGDTNYLLMDSNSSKGAPGSHVLYAST
                     DDGVTWKKRSTLSLDAEAWYGALCIGADGRL"
     gene            1160..2464
                     /locus_tag="VFB89_12005"
     CDS             1160..2464
                     /locus_tag="VFB89_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28077.1"
                     /translation="MRRWLAPILILVLTAAASAADRFLEVTYPPSDKPGELQLGVQYT
                     IWIPDGVEKLRGIIVHQHGCGPGACKAGATAAYDLHWQALARKWDCALLGPSYQQDEK
                     QNCRLWCDPRNGSDQTFLKAIAEFAEKSGHPELARVPWCLWGHSGGGFWASLMQMKYP
                     ERIVAIWFRSGTAFPVWEKGEIAKPDIPPDAMSIPMMVNPGIKEKSDQRFSGAWTGGE
                     AMFRAYRNKGAPIGFAPDPRTGHECGDSRYLALPFFDACLAQRLPKRGSANQTLKPVD
                     LKSAWLAPPLGGQPKPAAEFTGDAAGASWLPDKRVAQAWTEYVENGTVSDTTPPPAPT
                     NVKPVEKDGGVEITWEARADPESGIQCFLIQRDGVTVGQVPEKPVGRFGRPLFQSMSY
                     HDTPDKPLPEMRFIDKQVQPGRKHKYRLIAVNGVGLKSKPGR"
     gene            2570..3832
                     /locus_tag="VFB89_12010"
     CDS             2570..3832
                     /locus_tag="VFB89_12010"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZM28078.1"
                     /translation="MTPSPRYKWILVVVMLLISALNYGDRAALSAVFPMLRRDLGVTD
                     MELGLVGSAFLWAYGLGCPFAGLLADRFSRSRIIVISLVLWSLATLAVAPVRGLSDLL
                     TARIVFGVTQCLYVPASLALIADHHGPATRGVAMSLNIAGMSGGMIAGGTLTGYIAQT
                     WGWRYAFSLLGCLGLVLAVAAAFLVRDGEAPAAAAASSEKTSPIKNLRLLARTPTYQL
                     ILLQSMLNSAGVWMFWQWLPLYYQETYQLSLAGAGFSGTFMLQGTALVGILLGGYASD
                     RVGQKNVRRRPLIMAICYFCAAPILLVFLAQPGYALLSASVALFSLVRAFGQANENLI
                     MCDLLPPRARSTALGIFLMANVGAGSITILLAAWLRGLHGLGFAFACISGIVLLAAFS
                     ALAAYYLLPRDLARSQQPLAAINEGRVA"
BASE COUNT          668 a         1222 c         1200 g          762 t
ORIGIN      
        1 agccggccgt ccgccccgat gcacaaggcg ccgtaccacg cttccgcgtc caatgacaac
       61 gtgctccgct ttttccatgt caccccgtca tcggtgctgg cgtacagcac atgcgacccg
      121 ggcgcgccct tcgacgaatt actgtccatg agcaagtagt tcgtgtcgcc ggccaccgcc
      181 actgaacacg ggtagccgac gaaatcgccg tccaagggtg catagtcggt ccaggtcgct
      241 ccgtggtcgg cactgcgcat cacgccgttg ccggtccgct gattgttgtc gaaccgggtg
      301 aggaccaaga tcacggtgcc tcgccgggtc accagtcctt cctccaccca aacgggtcgc
      361 ttcgggtcgg cctgataggc ggcgtaggaa tacttgaact tgttgtatcg cgcccaggtc
      421 tttcctttat ccttgctgac ggcgtattca ctccacccgt cgagattgtg gtcgctggta
      481 ttggcataaa acgccaccag atcgccgttg gcgtactcca tggcgaccgg accgccgtgt
      541 gaacagcggc cggggtggtc gtgttccatg tcggcgatca gcgtcccgaa caccgcgtcg
      601 cccaccttcg tcgccggatc gtaatcgtag ataatcgcgc ctttgagcgg ctgctcgtcc
      661 tttccagcgg cggccgcaag cccgacgacg acggccgcac tcaaacccat taccaaccat
      721 ttcttcttat acatggcatg gtccattacc agtctcctgt gagcattgac tctgtccctg
      781 atttctccga gtgtaaccgg tcattcgacg tccgcctaga gaataggaaa gcattgttcg
      841 gggcttccgg agagactctg acttgagata ctggtgcggg tcgtcgactc aaatcgcgtt
      901 ctatgccgtc acaggtcaga aaattccggc tcagccaaca agatctagct ttcgacctga
      961 cagttagcct aagctcgaaa gcgaggagca aaccgacagt ggtcatgaag cgcgatggag
     1021 tctggcgggg ggaaggttta atgaatgccc ggaggttgac gatagaacgg caactcttcc
     1081 tggacgacat ggacttgctg cgcccggacg ctcaccgtca ggtgagcgac ctggtagcat
     1141 ctctcaaggc aaagtaatca tgagacgctg gcttgcccct attttgatcc tggtcctgac
     1201 cgcggccgcc tctgccgccg accgctttct ggaagtgacc tatccgccca gcgacaagcc
     1261 gggcgagctg caactgggcg tccaatacac gatctggatt ccggacggtg tcgagaagct
     1321 gcgcggcatc atcgtgcatc agcatggctg cggtccgggc gcctgcaagg ccggcgccac
     1381 cgccgcctac gatctgcact ggcaggcgct ggcccgcaag tgggactgcg ccttgctggg
     1441 accctcctac cagcaggacg agaagcagaa ctgcaggctc tggtgcgatc cccgcaacgg
     1501 ctcggaccag acctttctca aggcgatcgc cgagttcgcc gagaagtccg ggcatcccga
     1561 gctggcccgc gtcccctggt gcctgtgggg tcactccggc ggcggcttct gggccagcct
     1621 gatgcagatg aaatatccgg agcggatcgt ggccatctgg ttccgctccg gcaccgcctt
     1681 ccccgtctgg gaaaagggag agatcgccaa gcccgacatt ccccctgacg ccatgagtat
     1741 cccgatgatg gtgaatcccg gcatcaagga gaagagcgac cagcgcttca gtggcgcctg
     1801 gacgggaggg gaggccatgt tccgcgccta tcgcaacaag ggcgcaccca tcggttttgc
     1861 gccggatccg cgcacggggc acgagtgcgg tgattcgcgc tacctggccc ttcccttctt
     1921 cgatgcctgc ctggcgcagc ggctgcccaa gcgcggaagt gcgaaccaga cgctgaagcc
     1981 agtcgatctg aaatcagcct ggctggcgcc gccgcttggc ggccagccta agccggccgc
     2041 ggaattcacc ggcgatgcgg cgggggcgag ctggctgccc gacaagcggg tggcccaggc
     2101 ctggactgag tatgtcgaga acgggacggt gagcgacacc acgccaccgc ccgctcccac
     2161 gaacgtcaag ccggtcgaga aggatggcgg ggtcgaaatt acctgggagg cgcgggccga
     2221 cccggagagc ggcatccaat gtttcctgat ccagcgtgac ggcgtgaccg tgggtcaggt
     2281 gccggagaag ccggtgggtc gcttcggccg gcccttattt caatcgatgt cgtaccacga
     2341 cacgccggac aagccattgc cggagatgcg gttcatcgac aaacaggtcc agcccggacg
     2401 gaagcacaag taccggctga tcgccgtcaa cggcgtgggg ttgaagtcca agccggggcg
     2461 ttgacaccac gccaacacct gtacctccgg accggcaatt ccagctttca ggcgcagcaa
     2521 acactgatat gatccagggg catctccaga ctcgaggcaa actagcgtga tgacgccctc
     2581 cccacggtac aaatggatcc tggttgtggt tatgttgctg atctccgcct tgaactacgg
     2641 agatcgagcg gccctttcgg ctgtgtttcc gatgctgaga cgggatctcg gcgtcacgga
     2701 catggagctc ggtctggtcg gcagcgcctt cctctgggct tatggcctgg ggtgtccgtt
     2761 cgcgggcttg ctggcagatc gcttctcgcg cagccgtatc atcgtgatca gtctggtgct
     2821 ctggagcctc gccacgctcg cggtggcgcc tgtgcgcggc ctgagcgacc tgctgacggc
     2881 gcggatcgta ttcggtgtca cccagtgcct gtatgttccc gcctccctgg ctctgattgc
     2941 cgatcatcac ggaccggcca cccgcggtgt cgcaatgtct ttgaatattg ccggcatgag
     3001 cggcggaatg atcgccgggg gaaccctgac cgggtacatc gcacaaacct ggggttggag
     3061 gtacgccttc tcgttgctcg gatgcctggg cctggtactg gccgtcgcgg ccgcgttcct
     3121 ggtccgcgac ggggaagctc cggccgcggc cgcggcgagc tcggagaaaa cctcgccgat
     3181 aaaaaacctc aggctcctgg cgcggacgcc gacctaccag ctgattctgc tccagtcgat
     3241 gctcaattcc gccggggtct ggatgttctg gcagtggctg cctctgtact atcaagagac
     3301 ctatcagttg agcctggccg gcgccggttt ttcggggacc ttcatgctcc aggggaccgc
     3361 gctggtcggc atcctcctgg gcgggtacgc ctcggatcga gtcggccaga agaacgtgcg
     3421 gcggcggccg ctcatcatgg ccatctgcta cttctgcgcc gccccgatcc tgctggtgtt
     3481 cctggcgcag cctggctatg ccctgctgag cgcgagtgtg gccctcttct cgctcgtgcg
     3541 ggcattcggg caggcgaacg aaaatttgat catgtgcgac ctgctcccgc ccagggcgcg
     3601 gtcgaccgcg ctcggcatct tcctgatggc gaacgtcgga gcgggcagca ttacgatcct
     3661 gttggcagcc tggcttcgcg gattacacgg cctggggttc gcctttgcgt gcatctccgg
     3721 gatcgtactg ctggctgcat tttcggccct ggcggcctat tatctgttgc cgcgcgatct
     3781 ggcgcgctcc cagcagccgc tcgccgcaat caatgagggg cgggttgcgt aacactcgag
     3841 gtctacgcct tc
//
LOCUS       DASRJQ010000522         3440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3347201, whole genome shotgun sequence.
ACCESSION   DASRJQ010000522 DASRJQ010000000
VERSION     DASRJQ010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3347201"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /locus_tag="VFB89_12015"
     CDS             <1..1141
                     /locus_tag="VFB89_12015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZM28079.1"
                     /translation="PAMRALMAHSVEVWESDPEAFSYHPVGYMQISPEVMHADVAAIH
                     RQQQEIGYTSVFVEGEAECRRYMQDIFHDWQARGITSVLHEKKGGYANNRSSMNGLAG
                     KVRGEGVRILPGVEVKDFQSTGTAISGVVTDRGLVQCDYLVVAVGPWIPTLWAMLDLP
                     PAIGIRGRDGRLHENVQMWTYWCLQEGTLGVDPKLGLTNDGRMPPVIHVDTDAPLYSD
                     IDRSLITDKLWGLYYKPDFSFNGVQGGSVPYKVDKNPREVRIDPYGPESPEFVVGEDF
                     AKTWSSMLAFCQKRFEGKYPLYRKEPSGGLGAFTPDSFPVFDVFRENCYVIADSNHGY
                     KMIGIGKLVAGEIIGTKSSLLEPFRFSRYAEGKLHPVSHSPFPWS"
     gene            1203..2669
                     /locus_tag="VFB89_12020"
     CDS             1203..2669
                     /locus_tag="VFB89_12020"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HZM28080.1"
                     /translation="MTDLESYVNAEGRDEEVKRVRSKIDELGIEYVYYQFVSVTGRIM
                     GKGIPADHWDSVAQRGFQLVYGSTANLFMDRHRRYIAYGPEASELVGIPDPETFVQLP
                     WDGRVARVFCTLFRNREESEDPAAFLTSDCRGNLRRIHAEFQKKHKGLHLRHGCEPEM
                     MWLKKGEDGKPAGGFSKPYCYHIDQFESLRPVSLKVIDYARRMGLDMIQGDHEDAPGQ
                     LELNFMFDDALRTCDRLTTYRQICAQVAREFDLIACFMTKPFMGVSASGCHHNISLWY
                     GGEEAVNPLGQEKPAGMEGVFTYRKGGENTFLPRSGSRKPGPIGLNCIGGVIEHLPAL
                     TAIGCSTVNSYRRLWDTGFWAPVYSDWGYQNRTCGLRVSAPGRFEYRAVDAMVNPYLM
                     AAAILKAFDDGIRRKLDPGEPEQRNIYEAIKQGKQVRKLPLSLGEALQRLEQDKVIAS
                     ALPGDMYRVFMEYKRDEWERFLATVTDWDVTTYLDYLP"
     gene            2748..>3440
                     /locus_tag="VFB89_12025"
     CDS             2748..>3440
                     /locus_tag="VFB89_12025"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28081.1"
                     /translation="MTAMLQSLKHRGPDSTGYAVYGPPTAGCYVMRFKVAEQEDLRKG
                     FEIHQLIRQRRTEVDHRIESLGAEVLEAQEATEYAYRYRVRWQGELRRFCDYLEDVEG
                     VEILSIGNSLELVKDLGDARTVSGQYHLDGFIGTHAIGHVRMATESDVDIRSAHPYWA
                     YPYNDIAVVHNGQITNYWIMRREMERLGHRFMSNCDSELLAVYTANNLEQGATLLDSL
                     KASIKDIDGVFTY"
BASE COUNT          666 a         1066 c         1090 g          618 t
ORIGIN      
        1 gccggccatg cgcgccctga tggcccatag cgtggaggtg tgggagagcg acccggaggc
       61 cttcagctac caccctgtcg gctacatgca gatcagcccc gaggtgatgc acgcggatgt
      121 cgccgcaatc caccgccagc agcaggagat cggttacacg tcggtcttcg ttgagggcga
      181 ggccgagtgc cgtcgctaca tgcaggacat cttccacgac tggcaagccc ggggaatcac
      241 ctcggtgctg cacgagaaga aggggggcta cgccaacaac cggagctcga tgaacgggct
      301 cgccggcaag gttcgaggcg agggcgttcg catcctccca ggcgttgagg tcaaggactt
      361 ccagtctacc ggaactgcga tctccggcgt cgtcaccgat cgaggtctgg tccagtgcga
      421 ttacctcgtc gtcgccgtcg ggccctggat ccccaccctg tgggccatgc tcgacttgcc
      481 gcccgccata gggatccgcg ggcgcgatgg caggctgcac gagaacgtgc agatgtggac
      541 gtactggtgt ttgcaggaag ggacgctcgg agtcgacccc aagctcggcc tcaccaacga
      601 cggccgcatg ccgcctgtga ttcacgtcga cacagatgcg cccctctatt ccgacatcga
      661 ccgttcgctg atcacggata agctctgggg gctttactac aagcccgatt tctcgttcaa
      721 cggcgtccag gggggctccg tcccttataa ggtcgacaaa aatccccggg aagtgcggat
      781 cgatccctac ggccccgagt cacccgagtt cgtggtgggt gaggattttg ccaagacatg
      841 gtcttccatg cttgcgttct gccagaagag gttcgagggg aagtacccgc tctaccggaa
      901 ggagccctcg ggaggactgg gtgccttcac gccggacagc ttcccggtct tcgatgtctt
      961 ccgggagaac tgctatgtca tcgccgactc gaatcacgga tacaagatga tcgggatcgg
     1021 caagctcgtg gccggcgaga tcatcgggac gaagagctcc ctgctggaac cgttccgctt
     1081 ttcgcgctat gcggagggca agctccaccc cgtctcccac agcccatttc cctggagtta
     1141 ggcgagtctg atagagtcgc gggacgaacg ccggtccgcg tgtacggtga aaggagtgat
     1201 gagtgaccga tctcgagtct tacgtcaatg cggaaggccg cgacgaagag gtcaagcgag
     1261 tccgttccaa gatcgacgag cttggaatcg agtatgtcta ttaccagttc gtttcggtca
     1321 ccgggcgcat catggggaag gggatcccgg ccgaccactg ggatagcgtc gcccagcgcg
     1381 ggtttcagct cgtctacgga tcgacggcca acctgttcat ggaccgccac cggcgttaca
     1441 ttgcctacgg tcccgaggcc tccgagctcg tcggcattcc ggatccggag acgttcgtcc
     1501 agctcccgtg ggacggtcga gtcgcccggg tcttctgcac gctcttccgt aaccgcgagg
     1561 agagcgagga tccggctgct ttcctgacct cggactgccg cggcaacctg cgccggatcc
     1621 acgccgagtt ccagaagaag cacaagggac tgcacctgcg ccacggctgc gagcctgaga
     1681 tgatgtggct caagaaaggc gaggacggaa agcccgccgg cggattcagc aaaccttact
     1741 gctaccacat cgaccagttc gaaagcctgc gccccgtctc gctcaaggtg atcgactatg
     1801 cccgccgcat ggggctcgac atgatccagg gcgaccacga agatgcgccc gggcagctcg
     1861 agttgaactt catgttcgac gatgcgctgc gcacctgcga ccgtctgacg acctaccggc
     1921 aaatctgcgc gcaggtggcg cgcgagttcg acctcatcgc gtgtttcatg accaagccgt
     1981 tcatgggggt atcggcgtcg gggtgccacc acaacatttc cctctggtat ggaggcgagg
     2041 aggccgtcaa tcccctcggt caggagaagc cggcgggaat ggaaggcgtc ttcacctatc
     2101 gcaagggagg tgagaacaca ttcctgcccc gctccggtag caggaagccg ggaccgatcg
     2161 ggctcaactg catcggcggc gtgatcgagc atcttcccgc cctgaccgcc atcggctgct
     2221 cgacggtcaa ttcgtaccgc cgcctctggg acaccggctt ctgggctccc gtctactccg
     2281 actggggcta tcagaaccgc acctgtggac tgcgcgtgtc cgccccaggc cggttcgagt
     2341 accgggccgt cgatgcgatg gtcaatccct acctgatggc cgcagccatt ctgaaagcct
     2401 tcgacgacgg catccgccgg aagctcgacc cgggcgagcc ggagcagcgc aacatctacg
     2461 aggcgatcaa gcaaggcaag caagtgcgga agctgccgct gtcgctcgga gaagcgctcc
     2521 agcggctcga gcaggacaag gtgatcgcga gcgcgcttcc gggggacatg taccgcgtgt
     2581 tcatggagta caaacgggac gagtgggaac ggttcctggc cacggtgacc gactgggacg
     2641 tcaccaccta cctcgactac ttgccataat tctgggatta aaggacgatt atgtgcggca
     2701 tagcgggaat agtgcaccgg aacaggacgc acaacgtcgg ggcggagatg acggccatgc
     2761 tccagtccct caagcatcgg ggtcccgact ctaccggtta cgccgtctac ggtcccccca
     2821 ccgcgggctg ctacgtcatg cggttcaagg tggcggagca ggaggacctg cggaagggct
     2881 tcgagatcca ccagctgatc cggcagcggc ggaccgaggt ggaccaccgg atcgagtcgc
     2941 tgggcgccga ggtcctcgag gcgcaagagg cgacggagta cgcgtaccgc taccgcgtgc
     3001 gctggcaggg cgagctgcgg cgcttctgcg actatctcga ggacgtggag ggggtcgaga
     3061 ttctctcgat cggtaactcg ctcgagctgg tcaaggatct cggcgacgcc agaactgtca
     3121 gcggccagta ccatctcgac ggcttcatcg gcacgcacgc catcgggcac gtccggatgg
     3181 cgaccgaatc cgacgtcgat attcgctcgg cgcatcctta ctgggcatat ccgtacaacg
     3241 acatcgcagt cgtgcataac ggccagatca ccaattactg gatcatgcgc cgcgagatgg
     3301 agcggctcgg ccaccgattc atgtcgaatt gcgattccga gctgctcgcg gtttacaccg
     3361 ccaacaatct ggagcagggc gctacgctcc tggattcgct gaaggcgtcg atcaaggaca
     3421 tcgatggcgt gttcacctat
//
LOCUS       DASRJQ010000523         2758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2262333, whole genome shotgun sequence.
ACCESSION   DASRJQ010000523 DASRJQ010000000
VERSION     DASRJQ010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2262333"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1679
                     /locus_tag="VFB89_12030"
     CDS             <1..1679
                     /locus_tag="VFB89_12030"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HZM28082.1"
                     /translation="PAAQLKAVADGYLAARLAAFPEIATQFGIPNSRHDQLRDVTAAG
                     EQAWYKQEDRWLFELRRIDANALRGRPDWITYGLLREELEASKGVRVCRNRLWTVHPL
                     TGWQATYAALAQQQPVGTADLRGQSLARWRKLPKLVNGEIANLREGLKLGYSEPAVNV
                     RRVIDGMDKLLSTSPTESPFYNPATRDSTPEFQAALGELVADSLTPAMRRYRAFLNDE
                     YLPKARASIGIDGNPNGAACYRAAIRRFTTLDLSGEEIHQIGLREIAAAESSAAVLAE
                     RSFGSKDYRAVKRRLQADSAQTFGSRDKVIPVIDSVLDRAWAAAPKWFGIVPRSKLAV
                     EPFPAFQEPSVPMGQYLRAALDGSRPGIYRVNLYLATKPGARLEIDRLTFHEGVPGHH
                     FQLAIAQEREGVHPVTRYLSNSAFTEGWGIYSERLADEMGLYTSDAERLTDLEGMIYA
                     FATLVMETGMHTKGWTREQAIAYELEHTTRSEEQAALDVDRRIGWPGQGLSYTIGYLE
                     IHRLRALAQKEQGNHFDIRAFHDRVLEDGSVTLGMLREKLERWARTGPPT"
     gene            complement(1695..2477)
                     /locus_tag="VFB89_12035"
     CDS             complement(1695..2477)
                     /locus_tag="VFB89_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZM28083.1"
                     /translation="MSFTSLLYDVDDGIARVTINRPDKLNALSATVIGELADAVGHIE
                     ADAGVRGVILTGAGAKAFAAGADISELVKQGPMEAKERSVAGQRMMRRFERCGKPVIA
                     AVNGFALGGGCELAMACHLRVASESAKFGQPEVKLGLGPGYGGTVRLPRLVGKGRALE
                     LLLTGATIDAQEAYRIGLVNRVVPAEQVLPESEQLLKTILEQGPIAVRLVLEAVETGL
                     DLTVEEALLLEANHFGLLSSTADMREGTSAFLAKRKPEFKGR"
     gene            complement(2520..>2758)
                     /locus_tag="VFB89_12040"
     CDS             complement(2520..>2758)
                     /locus_tag="VFB89_12040"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZM28084.1"
                     /translation="KGDLLVVHGSGDDNVHYQGTERLINALVAANKPFTMMTYPNRTH
                     CICEGPGTTRHLFSLLERYLHEHMPAGATVAASR"
BASE COUNT          491 a          897 c          875 g          495 t
ORIGIN      
        1 atccagccgc gcagctgaag gcagtagccg acgggtacct cgctgcacgg ctcgccgcgt
       61 ttcccgagat cgccacgcag ttcggcattc cgaactcgcg tcatgaccag ctgcgcgacg
      121 ttactgccgc gggagagcag gcctggtaca agcaggagga ccgctggctg ttcgagctga
      181 gacgcatcga tgcgaacgct ctgcgcgggc gccctgattg gattacctac ggcttgctac
      241 gggaagagct ggaggccagc aagggcgtgc gggtctgccg gaatcggctg tggacggtgc
      301 acccgctgac gggctggcaa gcaacttacg ccgctctcgc tcagcaacag ccggtcggga
      361 ctgccgacct tcgcgggcag tcactggcgc gctggcgcaa gctgcccaag ctggtcaacg
      421 gtgagatcgc gaatctgcgc gaaggcctca agctggggta cagcgagccg gcggtaaacg
      481 tgcgccgcgt catcgacggc atggacaaac ttctctccac gtctccgacg gagtcgccct
      541 tctacaatcc ggccacccgc gactcaaccc ccgagttcca ggcggccctg ggcgagctcg
      601 tcgccgattc gctgacgccc gccatgcgtc ggtaccgcgc gtttctgaac gatgagtatc
      661 tgcccaaggc acgtgcgtcg atcggaatcg acggcaatcc caatggagcg gcctgctatc
      721 gtgcagccat tcgccgattc accacactcg atctgagcgg agaggagatt catcaaatcg
      781 ggttgcgcga gatcgctgcc gcggagtcca gtgccgcggt cctggccgag cggtccttcg
      841 gcagcaagga ctacagggcc gtgaagcggc gcctccaggc ggactcagca cagactttcg
      901 ggtcgcgtga caaggtgatt cccgtgatcg acagcgtgct cgaccgggcc tgggccgcgg
      961 cgccgaagtg gttcggcatc gtgccccgaa gcaagctcgc cgtggaaccc ttcccggcgt
     1021 tccaggagcc atcagtgccg atgggccagt acttgcgcgc tgctctggat ggcagccggc
     1081 cggggatcta tcgggtcaac ctctaccttg ccaccaagcc cggcgcccgg cttgagatcg
     1141 accggctgac ctttcacgaa ggcgtgcctg gtcatcattt ccagctcgcg atagcgcagg
     1201 agcgggaagg ggtgcacccg gtgacccgct acctctcgaa ttccgcattt accgagggct
     1261 gggggatcta ctccgagcgg ctggccgacg agatgggact ctacacctcg gatgccgaac
     1321 gcctgaccga tttggaggga atgatctacg cattcgccac gctggtgatg gaaaccggca
     1381 tgcacaccaa gggctggacc cgggaacagg cgatcgcgta tgagctggag cacacgactc
     1441 ggtcggaaga gcaggcggcg ctcgatgtcg atcgccggat cggttggccg gggcagggct
     1501 tgtcctacac catcggctat ctcgagatcc accggttacg cgcgctggct cagaaagaac
     1561 agggcaatca cttcgacatt cgagccttcc atgaccgcgt gctggaggac ggaagcgtca
     1621 ctctaggcat gttgcgggaa aagctcgagc ggtgggcgcg gaccggtcca cccacctaag
     1681 tcccagctcg attctcagcg acccttgaac tccggcttcc gcttggcgag gaacgccgag
     1741 gtgccttcgc gcatgtcggc ggtggacgac aacaggccga agtgattggc ctcgagcagc
     1801 agagcctcct cgacggtcag atccagcccg gtctcgaccg cctcgagcac caaccgcacc
     1861 gcaatcggtc cctgctcgag gattgtcttg agtagctgct cgctctcggg gaggacctgc
     1921 tcggccggca ccacccgatt caccagaccg atccgatagg cttcctgcgc atcgatggtc
     1981 gctccggtga gcagcagctc aagggcgcgg cccttgccta ccaggcgggg caggcgcacc
     2041 gtgccgccat atccgggtcc aaggccgagt ttcacctccg gctggccaaa cttcgcgctt
     2101 tcgctcgcca cgcgaagatg acatgccatc gccagctcgc agccgccgcc cagcgcgaat
     2161 ccgttgaccg ccgctatgac gggtttccca cagcgctcga atcgccgcat cattcgctgc
     2221 ccggcgaccg agcgctcctt ggcttccatc ggcccctgct tcaccaactc ggagatgtcc
     2281 gcgcccgccg cgaatgcctt cgcacctgcg cccgtcagga tgacaccccg taccccggcg
     2341 tccgcctcga tatggccgac ggcgtcggcc agttcgccga ttacggtcgc gctcagcgcg
     2401 ttcagcttgt cgggtcggtt gatcgtgacc cgtgcgatac catcgtcgac gtcgtagagc
     2461 agagaagtga aggacatggc ggtctcggtt agggagctat cagctatcag ctgtcagctt
     2521 cagcggctcg cggcgacagt agctccggcc ggcatgtgct cgtgtagata ccgctcgagc
     2581 aagctgaaga ggtgtcgggt cgtgcccgga ccttcgcaaa tgcagtgggt ccggttggga
     2641 taggtcatca ttgtgaatgg tttgttcgct gcgaccaatg cattgatcag tcgttcggtg
     2701 ccctgataat ggacgttgtc gtcgccgctg ccgtgaacta cgagcaggtc tcccttca
//
LOCUS       DASRJQ010000524         2033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_920755, whole genome shotgun sequence.
ACCESSION   DASRJQ010000524 DASRJQ010000000
VERSION     DASRJQ010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920755"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VFB89_12045"
     CDS             complement(<1..706)
                     /locus_tag="VFB89_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28085.1"
                     /translation="MLRGTLIALVVLAYAPAGAGAVGELAQKNGPAGCLSETVAECRG
                     ARALMAPVDIVVSPDGGSVYAVTTGSSSVTSFRRDPSSGELSQQPGGLGCVAPAGSVY
                     ECAVARHLSSPSAVAISPDGASVYVTDSSAPGGILTFDRDATTGALRQEPGRAGCILD
                     SEDPVPGDCAPGALLAEPRDVLVSPDGRSVFVATIYGVVGFARNPQTGALEQREGNGC
                     ITYGIAPCSAGRSIAPA"
     gene            976..1488
                     /locus_tag="VFB89_12050"
     CDS             976..1488
                     /locus_tag="VFB89_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28086.1"
                     /translation="MRRRPTYSCSSLVREECQRSPVSSRPRCRGARGKTRPDIPRGGA
                     NERDAHQGKHQARRLPPYLASWWRYTDLGIVLADEQHLAGRAEPPFVLPGGAPNRTRV
                     AAPGLECTGVAESVPARTETNATLGSGIGESEPGNAEDLGRTDADGAAVVDGMPVPTG
                     IPNNRPGMAV"
     gene            1496..1633
                     /locus_tag="VFB89_12055"
     CDS             1496..1633
                     /locus_tag="VFB89_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28087.1"
                     /translation="MAVLSIELPEHVLDALAERVAARVIQRLEYEQEGDRWLDAKQAA
                     D"
     gene            complement(1867..1939)
                     /locus_tag="VFB89_12060"
     tRNA            complement(1867..1939)
                     /locus_tag="VFB89_12060"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1904..1906),aa:Met,seq:cat)
BASE COUNT          365 a          718 c          659 g          291 t
ORIGIN      
        1 tggccggcgc gatcgacctt ccggccgagc agggggcgat gccgtacgtg atgcacccat
       61 tgccctcgcg ctgttcgagc gcacctgtct gcggattacg cgcgaacccg acgactccgt
      121 agatggttgc cacaaacacc gaacgcccat cggggctcac caagacgtcg cggggctcgg
      181 ccagcagcgc gccaggcgcg cagtcgcctg gtactggatc ctccgagtcc aagatgcatc
      241 ccgcccgccc gggttcttgg cgaagcgcgc ccgtcgtcgc gtcgcggtcg aatgtaagga
      301 tgccgcccgg ggcgcttgag tccgtcacgt aaacggatgc accgtcgggg ctgatggcca
      361 ctgcagaggg cgagctcaga tgtcgcgcaa ccgcgcactc gtagacggac ccagcgggcg
      421 cgacgcagcc cagcccaccg ggctgttggg agagctcacc gctcgacggg tcgcgccgga
      481 agctcgtcac cgagctcgac cccgtcgtca ccgcgtacac ggacccgccg tccgggctga
      541 cgacgatgtc cacgggagcc attagagccc gggctccgcg gcattcggcg accgtctcgc
      601 tcaagcagcc ggcaggcccg ttcttttgcg ccagttcgcc gacagctccg gcgcctgccg
      661 gtgcgtaggc gagaaccacc agagcgatga gcgtcccgcg tagcacgcct caggacccgc
      721 ggactgggcg gagcacgacg cgacagtagc gtcccccggc tgcacatgcc agccgccgac
      781 agaccgttct tgtagagcca ggcacagctg cgcttccaag accactggtc gatcgaagac
      841 ctacgcgacc cccggccacg cccggtcgtc cccgacgccg cgctcgagat caccgacgag
      901 cacgccgacg agccgcggct cttcctgatc gagtacgacc gcaccagcgc accgacaaga
      961 actacgcaga agagaatgcg tcgacgcccg acatattcgt gctcgtcgct ggtgcgtgag
     1021 gagtgccagc gcagcccggt cagttctcgg ccacgatgcc gaggtgcgag gggtaagacc
     1081 cgcccggaca tcccgcgagg cggggccaat gaacgcgacg cccatcaagg caagcaccaa
     1141 gcccgccggc tcccgcccta cctcgcctca tggtggcgtt acaccgacct gggcatcgtt
     1201 ctggcagacg aacagcacct cgcaggacgc gccgagccgc cgttcgtact cccgggtggg
     1261 gcgcccaatc gcacccgagt ggcggcacct ggcctggaat gcaccggtgt cgcagagtcc
     1321 gtccctgccc gcacagagac gaacgcgaca ctcggatccg gcatcggtga atcggagccg
     1381 ggcaatgccg aagatcttgg gcggacggac gctgacgggg ccgcagtcgt ggatggcatg
     1441 ccggtgccca ccgggatccc gaacaatcgt ccgggcatgg cggtctgaga gccagatggc
     1501 cgtgctctcg atcgagctcc cggaacatgt cctggacgct ctggccgagc gcgtcgcggc
     1561 gcgtgtgatc cagcgcctcg agtacgagca agagggcgac cgctggcttg atgcgaaaca
     1621 ggcggccgac tagctcggcc tgagcatcaa cgccctgcac aagctcacgg ccgcccggca
     1681 gcttcccgcc gagcaggaag ctccgggcca cacgctctgg ttccggcgtt cgcagctcga
     1741 cattggcgtt ctggaggcgg caacggcgct tccgcttcca aaacgcttcc atagagttcg
     1801 ctggaggcaa atcgcttgcg gccctgacaa ccgagaaaac cccgccgtag cggggctttc
     1861 ttaggatagc tccgcctgga ttcgaaccag ggacctcacg attatgagtc gtgcgctcta
     1921 accgctgagc tacggagccg aggccgctct gagtctagac acgcggcgca acccccggcc
     1981 gccctcgggc gaggttccta aaagcggcta tccggcgcca agcgcgagtg gca
//
LOCUS       DASRJQ010000525         2471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1282852, whole genome shotgun sequence.
ACCESSION   DASRJQ010000525 DASRJQ010000000
VERSION     DASRJQ010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282852"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..2421
                     /locus_tag="VFB89_12065"
     CDS             124..2421
                     /locus_tag="VFB89_12065"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM28088.1"
                     /translation="MPLMLGSRLGPYEIVAPLGAGGMGEVYRARDPRLKRDIAIKVLP
                     ADMASSPDRLARFEREAMTVAGLNHPNIVVLHSIEEDGGIRFLTMEMVEGRNLAELIT
                     PGGLPLAQLLDLGIPLADALVAAHEKGVVHRDLKPANVMVTRDGRVKVLDFGLAKLAQ
                     VESDADATQATTLAAPISDVGQVLGTVPYMAPEQIHGEAVDARTDLFSFGILLYELAA
                     GKRPFVGQTLADVCSAILHDTPSSLTSLRGDLPSELAHIVERCLEKRPRERFQTALDV
                     SNELRSVRRTLERGVPAQQSKSDKIASIAVLPFANRSRDETDEYFSDGLADELLSMLG
                     KIRGLRVAARTSAFHFKGKDVTIAEAGAALHVATVLEGSVRKAGNRVRISVQLVKVAD
                     GYHLWSETYDRTLEDIFAVQDDIAHSVVRELRTTLLGEEADPDASGQAREEVARAKKG
                     RTIDAEAYRLFLLARHLMDRFTREEMAKAIEYLKQALDRDPGFALGWAELGVGYSREA
                     EWHWVPAVEAYRRASAAVERALALEPDLAEGHAQMGWIRIHHDCDLHGAAASIARALE
                     LAPGDAIVLRTAGVLAWCQGRLDDAIRLWRRILEQDPLSAPVYQNLGWAFHSANRFRE
                     AEGAFRKALELTPHRVVTRATLSLTLLALDRGEEAVAEAIQEPHETFRLWAQAIVHHV
                     MNHGAKSEAALRELTEKHANDGAYRVAEAQAMRGETGAAFEWLERACAQRDSGLPKMK
                     LSPCLRLLHADPRWAPFLKRMGLDE"
BASE COUNT          429 a          757 c          877 g          408 t
ORIGIN      
        1 cgaccgtctg cgaagaagcc cggggtcgta gccgaacgtg gatcgggcgc tcacccctgg
       61 aggggtgaaa gggtaagccc gcctcgagcc cgtcgatcgt gctctctgag gtacattccg
      121 cccatgccac tgatgctcgg gtcccgcctc ggcccctacg agatcgtcgc gccgctcggc
      181 gccgggggga tgggagaggt ctaccgtgcg cgcgacccgc gcctcaaacg cgacatcgcg
      241 atcaaggttc tgcccgccga catggcgtcg tcgcccgacc gcctcgcccg cttcgagcgc
      301 gaagcgatga ccgtggcagg cctgaaccac ccgaacatcg tggtgctcca ctccatcgag
      361 gaagacggcg gcatccgatt tctcaccatg gagatggtgg aagggcggaa cctcgccgag
      421 ctcatcactc ccggtggatt gccgctcgct cagctgctcg atctcgggat cccgctcgcc
      481 gacgcattgg tggcggcgca cgagaagggg gtggtgcacc gcgacctcaa gcccgcgaac
      541 gtgatggtga cgcgggatgg gcgcgtcaag gtgctggatt tcgggttggc caagctcgcg
      601 caggtcgaat cggatgcgga cgccactcag gcgacgacgc tggcggcacc catctcggat
      661 gtcggccagg tccttggcac cgtgccctac atggcccccg agcagattca cggcgaggct
      721 gtcgacgcgc gtaccgacct gttctcgttt ggcattctcc tgtatgagct cgcggccggg
      781 aagcgcccgt tcgtggggca gacgttagcg gacgtgtgct cggcgatcct gcacgacaca
      841 ccgtcgtcgc tgacgagtct gcgtggggac ctcccgagcg agctggcgca cattgtcgag
      901 cgctgtctgg agaagcgccc gcgcgagcga ttccagaccg cgctggatgt ctcaaacgag
      961 ctgcggtcgg tccggcgcac gctcgagcgc ggcgtgccgg cgcagcaatc gaaatcggac
     1021 aagatcgctt cgatcgcggt gctgccgttt gcgaaccgca gccgagacga gacggacgag
     1081 tacttctccg acggcttggc cgacgagctg ctcagcatgc tcggcaagat ccggggcttg
     1141 agagtggctg cgcgcacgtc ggcgtttcac ttcaagggca aggacgtgac gatcgccgag
     1201 gccggggccg cccttcatgt cgctaccgtg ctggaaggca gcgtccgcaa ggccgggaac
     1261 cgggtgcgaa tctcggtgca actggtgaag gtcgcagatg gctatcacct gtggtcggag
     1321 acctatgacc gtacgctgga agacatcttt gcggtgcagg acgacatcgc gcactcggtg
     1381 gtgagggagc tacgcacgac cctgttgggc gaggaggccg atcctgatgc cagcgggcag
     1441 gcgagggagg aggtagcgag agcgaagaag ggacggacca tcgacgccga ggcgtaccgg
     1501 ctgtttctcc tggcccggca cctcatggat cgattcacgc gcgaggaaat ggcgaaggcg
     1561 atcgagtacc taaagcaagc gctggatcgg gatccagggt ttgcactcgg ctgggcggag
     1621 ttgggcgtgg ggtactccag ggaagcggag tggcactggg tcccagcagt ggaagcatac
     1681 cgacgtgcga gcgcggcagt ggagcgggct ctcgcgctcg agccggatct ggccgagggg
     1741 catgcgcaga tggggtggat cagaatccat cacgactgcg acctgcacgg cgcggcggcg
     1801 tcgattgctc gggcgctgga actggccccg ggagatgcca tcgtgctccg cacggctggc
     1861 gtattggcgt ggtgccaagg ccgcctcgat gatgcgatcc ggctttggcg ccggattctg
     1921 gagcaggacc cgttgagtgc acccgtctac cagaacctcg ggtgggcgtt ccactctgcg
     1981 aatcgcttca gggaggcgga gggggcgttt cgcaaagccc tagagctcac cccgcaccgc
     2041 gtcgtcacgc gtgccacatt gtctcttacc ttgcttgctc ttgaccgggg tgaggaggcg
     2101 gtggcggagg cgattcagga acctcatgag acgttccgcc tgtgggccca ggcgattgtt
     2161 caccatgtca tgaaccacgg ggcgaagtcg gaagccgcgc tgcgagagct gactgaaaag
     2221 cacgcgaacg acggcgccta ccgggtggcg gaggcgcaag cgatgcgagg ggagacgggc
     2281 gcggctttcg agtggctgga gcgcgcatgc gcccagcggg acagcggact ccccaagatg
     2341 aagctcagcc cctgccttcg cctcctacac gccgatccgc ggtgggctcc gttcctgaaa
     2401 agaatggggc tcgatgagtg agcagcgcct cagcgcctgc ctcggcatcc gcggccgacg
     2461 gtgaatcgaa t
//
LOCUS       DASRJQ010000526         5297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_4020223, whole genome shotgun sequence.
ACCESSION   DASRJQ010000526 DASRJQ010000000
VERSION     DASRJQ010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5297
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4020223"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2515)
                     /locus_tag="VFB89_12070"
     CDS             complement(<1..2515)
                     /locus_tag="VFB89_12070"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HZM28089.1"
                     /translation="MHRAIPPDSAHASLPVREGGQHEAIGDMYFAADNFVAAAEAYQE
                     ALRCVPDRTMVDRLGLLLRISRCHFLRGDYPASHAVLLDARRLVRGTGAELSARVAAR
                     LGETLCLLGQYRRARRYANYAYGVLRNSDDHLTVGEVGVTIGICDYRLGRNRESIEWL
                     QDAAATFRRIDESDGLVNALNNLGIVYKNLGEWREASRFLEQALRIDERAGFYARMRG
                     HRQNLGLIRFRLGEWELATEDFRRSLQIARDTGHLAGEAMALMALGRLHRRRRQLGKA
                     EEHFRRAMSLAEQSGAERESDLAREFLAEVEIDRGNATAAATLLETTLEQARHRAPEG
                     DLVGELEIRLGLALSHLGRADDARTHLMAGSLIMQRLGDRLEEAIADRCFARLEAQAG
                     NAGLMDAKVRAAAQCFEQLGEQYELALTLGQWGEFILLLPAGTRSQVVLEPVAEATRR
                     AAALFREFGVPSLAAEAYLSLARLEAEREHFDQGLSLLEQAETWLEESEDATGEDRAA
                     ALRREIEHQYVAVSLSTSNEFRALEEANRLFRDTSDMAGLLAQTVKLAVEHAGGDRGI
                     VAYSHAGGRLDVVAQHGLGRDRARRILTTIESVAGRRIAESGPMFSSRVAADPRLSAA
                     LAGALEGVGSLICVPLNFPSQSLGVVYVDRFSDSLHGSFKQRDLNLLAVLANSAAVAI
                     VEAQRSLLLQENQELRKQLMPVPGVDRVVSHSREMEDILRLLAKVSDSSATVLFMGET
                     GTGKGLLAQVVHEISRRRDRALVQVNCAAIPEQLLESELFGYIHGAFTGALRDKSGLF
                     EEAEGGTIFLDEIEKVPESVQAKLLHVLDSGEIRPVGATR"
     gene            complement(2661..2960)
                     /locus_tag="VFB89_12075"
     CDS             complement(2661..2960)
                     /locus_tag="VFB89_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28090.1"
                     /translation="MQSVHRHKLPIVLVLAAFLGAMAWSVADAKPYEGFRKNTSGFIK
                     QKAGVLSGDPDSPTVNPPSGGTNQKSLIGWNGSDLETGSRVRTNWALLLWMWTRR"
     gene            complement(3106..4089)
                     /locus_tag="VFB89_12080"
     CDS             complement(3106..4089)
                     /locus_tag="VFB89_12080"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28091.1"
                     /translation="MRDSDGVRVCAVDVGSNSVRLLVADVRADGVVTAIVRAGEPCRL
                     GRGLHRSGMIEEPTAGRAASLILDFVQRGLSLGAQRVLIGATAALRDAANGEAVAARI
                     AKRCGFPVRILTGEQEARLVYEAVVTGLGGPARRSSCVVFDLGGGSTEVVSGFGPSPG
                     RSTSLPFGAVSLTERFLHANPPADNEIADLDGHVNRELESRCAGMPDETAVLAGVGGT
                     VTAFGVIDRGLRTYDPDLVEGWLIETARLEALVDKLVRTTEAERRSWTVLGSGRADIV
                     VAGARVVLLLTRRFRSRGLICSTRGLRYALARIAVAEGGEARPGSGDAGTV"
     gene            4224..5297
                     /gene="rlmN"
                     /locus_tag="VFB89_12085"
     CDS             4224..5297
                     /gene="rlmN"
                     /locus_tag="VFB89_12085"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HZM28092.1"
                     /translation="MSSAVFSTHPAASRPSLFGLTRHQLGQVLEERGFPRYRADQIFG
                     WIYGKHHRQPEHMTNLPASLRQTLQEVCDFGLPRVERLLSTPDQLTHKFVLELTDGAR
                     VECVSMRTDKRLTFCLSSQVGCALKCSFCATGLMGLKRNLRTHEIVAQVLLMGDLHRW
                     RDSRFNLVFMGMGEPLANLSPVLDSIRILHDTPGLGLGARRVTVSTSGVVPGIRELAA
                     EGLPVGLAVSLHATTDELRDQLVPINRRWPLRELLSAARDYGRTTGRRVTLEYTLMAG
                     VNDGPEDARRLAEIARGLPSKINLIPYNPVPGLEWKRPTTESMESFVRHLYPVAPSVT
                     LRNTLGGEIWAACGQLGGLSPAA"
BASE COUNT          880 a         1850 c         1711 g          856 t
ORIGIN      
        1 tacgggtcgc ccccaccggc cggatttccc cgctgtcgag gacgtgcagg agcttggcct
       61 gcaccgactc gggaaccttc tcgatctcgt cgaggaagat ggtgcccccc tcggcttcct
      121 cgaacagccc ggatttgtcg cgcagcgccc cggtgaacgc gccgtggata tagccgaaca
      181 gctcgctctc gagcagttgt tccggaatcg cggcgcaatt gacctgcacc agggcacggt
      241 cgcggcggcg cgagatctca tgaacgacct gggccaggag gcctttgccg gtccctgtct
      301 cgcccatgaa gagaacggtc gcggagctgt cgctcacctt ggccagcagc cggaggatgt
      361 cctccatctc gcgggaatgg ctgaccacgc ggtcgacgcc cggaaccggc atgagctgct
      421 tgcgcagctc ctggttttcc tgcagaagca gcgagcgttg ggcttcgacg atggccaccg
      481 ctgcgctgtt ggccaggacc gcgagaaggt tgagatcgcg ctgcttgaac gacccatgaa
      541 ggctgtcgct gaaacgatcg acgtagacca cgcccagcga ctgtgagggg aagttgagcg
      601 gcacgcaaat cagcgaacca acaccctcga gcgcgccggc cagggctgcg ctgaggcgcg
      661 ggtcggccgc cacgcggctg gagaacatcg gtccactctc ggcgatgcgg cgtccagcga
      721 cggactcgat ggtggtgagg atccggcgtg cgcggtcgcg tccgaggcca tgctgggcca
      781 ccacatcgag ccggccgccg gcgtgactgt aggcgacgat tccgcggtcg ccgccggcat
      841 gctcgaccgc cagcttcacg gtctgcgcca gcagcccggc catgtccgag gtgtcgcgga
      901 acaaccggtt ggcttcctcg agcgcgcgga attcgttcga agtcgacagc gagaccgcga
      961 cgtactgatg ctcgatctca cgacgcagcg cggcggcacg atcctcgccg gtggcgtcct
     1021 ccgactcctc gagccaggtc tcggcctgct cgagcaggga cagaccctga tcgaagtgct
     1081 cccgctcggc ctcgaggcgg gccaggctca gatacgcctc ggcggccaac gacggcacgc
     1141 cgaactcgcg gaatagggct gccgcgcgtc gcgtggcctc ggccaccggc tcgagcacca
     1201 cctgggaccg ggtcccggcc ggcagaagga ggatgaactc accccattgg cccagagtga
     1261 gggccagttc gtactgttcg ccgagctgct cgaaacactg ggcggccgcg cgcaccttgg
     1321 catccatcaa gccggcgttg ccggcctgag cctccaggcg cgcgaaacat cgatcggcga
     1381 tggcttcctc gagcctgtcg ccgaggcgtt gcatgatcag cgagcccgcc atcaggtgag
     1441 tgcgcgcatc gtcggcgcga cccaggtgcg acagcgccag gccgagtcgg atctcgagct
     1501 cacccaccag gtcaccctcc ggggcgcggt gacgagcctg ctcgagcgtc gtctccagca
     1561 aggtcgcggc ggcggtggcg ttgccccggt cgatctcgac ctcggccagg aactcgcggg
     1621 caagatcact ctcacgctcg gcccccgact gctcggcgag cgacatggct cgcctgaaat
     1681 gctcctcggc cttccccagc tggcggcgcc ggcggtgcag gcggcccagt gccatcaggg
     1741 ccatggcttc acccgccagg tgaccggtat cgcgcgcaat ctggagcgag cgtcggaagt
     1801 cctcggtggc cagttcccac tctccgaggc ggaagcggat cagtcccagg ttctgcctgt
     1861 ggccacgcat gcgggcgtag aaaccggcac gctcgtcgat tcgcagggcc tgctccagga
     1921 agcgggaggc ctcacgccac tccccgaggt ttttgtagac gatcccgagg ttgttgaggg
     1981 cgttcaccag tccgtcggat tcgtcgatgc gccggaatgt cgccgccgca tcctggagcc
     2041 actcgatgct ctcgcgattc cggccgagcc ggtaatcgca gatgccgatg gtgactccga
     2101 cctcacccac ggtcaggtgg tcgtcgctgt tgcgcagaac gccataggcg tagttggcat
     2161 agcgccgcgc ccgccgatac tggccgagca ggcacaacgt ctctcccaac cgggcggcca
     2221 ccctggcgct gagctcggcg ccggtgccac gcaccagtcg gcgcgcatcg agaagtacgg
     2281 cgtgtgaggc ggggtagtcg ccgcgcagga agtgacagcg gctgatacga agcaggaggc
     2341 cgaggcgatc gaccatggtc cggtcgggaa cgcagcggag cgcctcctgg taggcctcgg
     2401 cagcagccac gaagttgtcg gcggcaaaat acatatcgcc gatggcttcg tgctgcccgc
     2461 cttcccttac cggcaggctt gcatgagctg aatcgggggg gatcgctcga tgcacgttcg
     2521 aaccctcgac cttgtcaacg cccggcccgg acccctcggg ttcagccggg gacccgcaac
     2581 tctttccctt ggatggtgga cgcgtcctgc ggatttgagc ggagaacccg atcagcccgc
     2641 gatcgggaac ccgctctccg ctagcgtcga gtccacatcc acaacagcaa tgcccaattc
     2701 gttctgaccc tcgaacctgt ctcgagatcc gatccgttcc acccaatcag cgatttctga
     2761 ttggtgccgc cggacggcgg attgacagtc ggagaatccg gatcaccaga gaggaccccc
     2821 gctttctgct tgatgaagcc tgaagtgttc ttacggaagc cttcatacgg cttcgcgtcc
     2881 gcaacactcc aggccatcgc acccaggaag gcggccagga ccagaacgat cgggagcttg
     2941 tgacggtgaa ctgattgcat gcgtcgatgt actccgtctg tttgagtgag acgcaagcga
     3001 cagccgtccg ctcgtgagag cggtccgccg acacccgttc ggtgagaggg ggactcctgc
     3061 agcgccatgg ctagtatccg tccatccgca attggtcgtc aacggctaaa ccgtaccggc
     3121 gtcgccggac ccgggccggg cctctcctcc ttccgcgacc gcgatgcgag ccaaggcata
     3181 ccgcaggccg cgggtggagc aaatcagccc ccgcgaccga aatcgacggg tgaggagcag
     3241 aaccacccgt gcccctgcga cgacgatgtc ggcccggccg ctgcccagca cggtccacga
     3301 gcgtcgctcg gcctccgtcg tccgaaccag cttgtcgacc agcgcttcga ggcgggccgt
     3361 ctcgatcagc cagccttcga ccaggtccgg atcgtaggtc cgcagcccgc ggtcgatgac
     3421 gccaaaggcg gtgaccgtcc cacccacgcc tgcgagcaca gcggtctcgt cgggcatccc
     3481 ggcacaacgg ctttcgagtt cccgattcac atgcccgtcg aggtcagcga tctcgttgtc
     3541 tgcaggtggg ttggcgtgca ggaagcgctc cgttaggctg accgccccga agggcaggct
     3601 ggtgctccgg ccggggctgg ggccaaaacc gctcaccact tcggtgctcc caccgcccag
     3661 gtcgaataca acgcaggagc tacgccgcgc cgggcccccc agcccggtga ccacagcctc
     3721 gtaaaccagg cgggcttcct gttcgccggt caggatgcga accggaaacc cacagcgctt
     3781 ggcgatccgg gccgccaccg cctccccatt ggcggcgtcc cggagcgccg cggtagcacc
     3841 gatcaggacc cgttgcgccc caagactgag cccacgctgc acgaagtcca ggatcaggga
     3901 ggccgcgcgg ccggccgtgg gctcttcgat cattccggag cggtgcaggc cgcggccgag
     3961 acggcagggt tcgccagccc gcacgatggc ggtcacgacg ccatccgccc tcacgtcggc
     4021 caccaggagg cgcacggagt tcgatccgac gtcgacagca cacacccgca caccgtccga
     4081 gtcgcgcatg ggccgctctt accatcggcc gcgcgttgac cgcaaatgtc acgcttgcta
     4141 gcctccgctc tccctgctgc ccgctggggc acgcggcccc gacggggcgc caacgtaacg
     4201 tccgggaccc gaatcgcgac cccatgtcca gcgccgtctt ctcgacacac cccgcggcct
     4261 cgcgcccgag tctgttcgga ctgacccgac accagctcgg tcaggtcctc gaggaacgtg
     4321 gtttcccccg ctatcgggcc gaccagatct ttggctggat ctatggcaag catcaccgcc
     4381 agcccgagca catgaccaat cttcctgcct cgctgcgcca gacccttcaa gaggtgtgcg
     4441 acttcggact tccccgggtg gaacggctcc tctccacccc cgatcagctc acccacaagt
     4501 tcgtgctgga gctgacggac ggggcccggg tcgaatgtgt ctcgatgcga accgacaagc
     4561 gcctcacctt ctgtctttcc tcccaggtgg gctgcgccct caagtgctcg ttctgcgcca
     4621 ccggcctgat gggactgaag cgcaacctgc gcacccacga gatcgtcgcc caggtgctgc
     4681 tgatgggtga tctccatcgg tggagggatt cgcgcttcaa cctggtcttc atgggaatgg
     4741 gtgagccgct cgccaacctg tcgccggtac tcgacagcat tcgcatcctc cacgacaccc
     4801 ccgggctcgg tctgggcgcg cggcgggtca cggtctcgac cagcggagtg gtgccgggga
     4861 tccgcgagct ggcggccgag gggctcccgg tggggctggc ggtttccctg cacgccacca
     4921 ccgacgagct gcgcgaccag ttggtcccga tcaatcggcg ctggccgctg cgcgagttgc
     4981 tgtcggcggc gcgcgattac ggtcgcacca ccggtcgacg ggtgaccctc gagtacacgc
     5041 tcatggcagg ggtcaacgac ggtcccgagg acgcgcgccg tttggccgag atcgcgcgcg
     5101 gcctgccgag caagatcaac ctgatccctt ataacccggt cccgggactg gagtggaaac
     5161 gccccacgac cgagtccatg gagtccttcg tccgacacct gtaccccgtg gcgccctcgg
     5221 tgaccctgcg caacacgctg ggcggcgaga tctgggccgc gtgcggacaa ctgggtggcc
     5281 tcagccccgc ggcctga
//
LOCUS       DASRJQ010000527         5597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_898247, whole genome shotgun sequence.
ACCESSION   DASRJQ010000527 DASRJQ010000000
VERSION     DASRJQ010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898247"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VFB89_12090"
     CDS             <1..199
                     /locus_tag="VFB89_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28093.1"
                     /translation="NQAAATDDPVAAKKLYRRAMDALNADAPAIFLYSPTTTAAVSKR
                     LNGVEIDPYSWLNDLPTWGVR"
     gene            534..1124
                     /locus_tag="VFB89_12095"
     CDS             534..1124
                     /locus_tag="VFB89_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28094.1"
                     /translation="MRPWTRGLVSMLIAAALLAVGASCTSNDSIVGPSAEPSQQPTTP
                     PLQESTPPNPLLEPLVDPALQLTLKTVHLLTCSPQPYAQTKAVVGPAGGTIVVGAHRL
                     TIPAGALSSPVTITAEQMSGTVNSVRLQPAGLKFAKAATLTLSYSNCLLPPATKRMVY
                     TDEQLRILELIPSLDLRLSRSVNGSILHFSRYAVAY"
     gene            1129..2742
                     /locus_tag="VFB89_12100"
     CDS             1129..2742
                     /locus_tag="VFB89_12100"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZM28095.1"
                     /translation="MSTAPSRASLRPAGEAPPTAAATLLQRAREQELAGSLAEAINGY
                     EAAIAEAEMSGDARARSEAQRRLAVVRYRRGERDAAVALCEQSLAFATDLGDTVLAGE
                     ALNTLAGFDFESGAMDQARERFYHALELGGSSPELRGRIEQNLGILANIRGDHGEALA
                     HYQQSLFAFQRTGDDHGCAIAYHNLGMVSADRARWDEADEYFRRSLEITAAVGDVHLT
                     GLGLLNRSEVQLARQRYSDAKADAEAALRIFDRLGAKLDKADTYKVIGRVYRETGQLA
                     LAEARLTAAIELAVGTHSVLSEAEASRELALLYQAMGRNQDALRLLSAAHRLFGRLEA
                     RLDLVDVSGRVQRLEDTYLALVRDWGQSIESADSYTFGHCERVANYALAVAEALGMEE
                     AHRTTVRIGAYLHDVGKVRVPHEILNKPGPLSREEFEIIKLHPLWGVELLAGVDFPWD
                     IKPIIRWHHEKYDGTGYPDRLCGDEIPLSAQVICIVDVYDALTTTRSYRAPMSPGAAA
                     AQMHESRRWWRPDVYEAFRRAVPDVAEAA"
     gene            complement(2753..3154)
                     /locus_tag="VFB89_12105"
     CDS             complement(2753..3154)
                     /locus_tag="VFB89_12105"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZM28096.1"
                     /translation="MALEVFDAAVAGARPAQASEPDYTALGADVEGRLVGWICWGATP
                     CTLGTYDLYWMAVDPAGQHVGVGSALLAEMERRLVGRARLIVVETAGRADYADTRRFY
                     QARGYEATARIPDFYAPGDDLVVYTKRLVEV"
     gene            complement(3262..4272)
                     /locus_tag="VFB89_12110"
     CDS             complement(3262..4272)
                     /locus_tag="VFB89_12110"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28097.1"
                     /translation="MRAAIIYDAGSVEWSPQDVAAVLSNVHEVRTSLRRRDYDVELVP
                     VRLGEFRWLSRARKADVIVNLCEGINGHAKYEDLVVNTVELTGLPFTGCRAWPVTVCH
                     RKHVANTLLQSAGIPIPHFVLAQGNAIPADFPLPAIVKPAAEDASVGIDGNAVCTTRK
                     ALRKRVAQMTDQFEDVMIQEYVPGREINVGFVGKTMLPLSEIRFDSMPADTWPIVTYA
                     AKWTTGSADDLGTQPVCPAEIAPDLMKRLTHVAWRAWELLAGAEGYGRVDLRVTEDGQ
                     PYVLEVNPCPDLSSNAGLARMGQAFGWSYDELVHQIVDEALTRMQPSRFAADLVRGVP
                     AA"
     gene            complement(4269..5201)
                     /locus_tag="VFB89_12115"
     CDS             complement(4269..5201)
                     /locus_tag="VFB89_12115"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28098.1"
                     /translation="MENALATLGEVIRLEADQDFPAKLLDYRPDFVFNIAEGLFGPSR
                     ESHVPAICEFLGVPYHASDPLTLGLCLHKGRAKEMLERYGVPTAPFRLARTVAEARAT
                     RLPFPLFVKPAFEGSGKGVSSKSVCHSRAELTTQVNYLLATYREPVLIETYLPGREFT
                     VAILGNGDEARCLPIVGMCFDTLPPGAPPIYGYEAKWLWDTPSHPLDIFECPARIPES
                     LATAVREAALAAYHALDCRDWCRIDLRCDAAGRPMVVELNPLPGILPDPRDNSCFPKA
                     AAAAGMSYEELICTVADIAWRRISGGSLLAGVAS"
BASE COUNT         1021 a         1763 c         1834 g          979 t
ORIGIN      
        1 caatcaggcg gctgccaccg atgatcccgt ggccgcgaag aagctgtatc gccgagcaat
       61 ggatgcgctc aatgcggacg cacccgccat cttcctctac tcgccaacca ctacggcggc
      121 tgtgagcaag cgcctcaacg gtgtcgaaat cgacccgtat tcgtggctca atgacttgcc
      181 cacctggggg gtgcgatagg tattgttgca cgcttctgtt gcattgggct cacatgattc
      241 gagagtgcag ctcccggcat tgagctacgg aatccggtgg cctgaaagcg tacagacctt
      301 ccggcccact gctgccacaa catccgctcc ttcgcctcct tccgctgtca tccctttgtg
      361 ggtgtcactg tttttgacgc tgcaagtcgt gtagggcata ccggttgcag cttgacctcg
      421 gactgggggt tttccgcgta ccggcggcga gagaacaacg gactcgctgt cggcggtcgc
      481 gcgagcggcc gcggtaccgt cgcatggcga taacatcagc ggggggtgct ccgatgcgtc
      541 cgtggacacg tgggctggtt tccatgctta tcgctgccgc cctcctggcg gtcggcgcga
      601 gctgcacatc caacgactcg attgtcgggc cgtcggcaga gccgtcgcaa cagcccacca
      661 caccgccatt gcaggagtcg acgccgccca atcctctatt ggagcccctg gtcgatccgg
      721 ctctgcaact cacgcttaag acagttcacc tgctcacctg ctcgccacag ccatacgccc
      781 agaccaaagc cgtggtgggg cctgcaggtg gcaccatagt ggtcggcgcc caccggctta
      841 cgatccccgc cggtgcgttg tcgtctcctg taacgattac cgccgagcag atgagcggca
      901 cggtcaactc ggtccggctt caaccggctg ggctcaagtt cgccaaagcc gcgaccctga
      961 cgctcagcta cagcaactgc ctactccctc cggcgaccaa gcgaatggtc tacaccgacg
     1021 aacagctccg cattctggag ctgattccgt cgctggatct acggctgagc cggagtgtca
     1081 acgggagcat cctgcatttc tcacggtacg cggttgcgta ctgatcccgt gagcactgcg
     1141 ccctcccgag catcgctgcg gccggcgggt gaagccccgc cgacggccgc agcgacgctc
     1201 ctacaacgag cacgggagca ggaactcgca ggcagcctgg ccgaagccat caacgggtac
     1261 gaagcggcga tcgccgaggc cgagatgagc ggtgatgcca gggcgcgctc agaagcgcaa
     1321 cggcggctgg cggtggttcg atatcgtcgg ggggagcgcg acgcagccgt cgcgctctgc
     1381 gagcagagcc tggctttcgc cacggacctc ggtgacacgg ttctggcggg cgaggccctg
     1441 aatacgcttg ccggattcga ctttgagagt ggcgccatgg atcaggcgcg ggagcggttc
     1501 tatcacgccc tcgagctggg tggctcgagc ccggagctgc gaggtcggat cgagcagaac
     1561 ctgggtatcc tcgcgaacat ccggggcgat catggggagg ccctcgcaca ctaccagcaa
     1621 tcgctgtttg cgttccagcg gaccggcgac gaccacggct gcgctatcgc gtatcacaac
     1681 ctcgggatgg tgagcgcgga ccgggccagg tgggacgaag ccgacgagta cttccggcgg
     1741 tctctcgaga tcaccgccgc cgtgggtgat gtgcacctca cgggactggg cctgctcaac
     1801 cggtccgagg tccaactggc gcgccagcga tactctgatg cgaaggccga cgcggaggcg
     1861 gccctgcgga tcttcgatcg gctgggcgcc aaactcgaca aggccgacac ctacaaagtg
     1921 atcggtcggg tctaccgcga gacgggccag ttggcgctgg cggaagcccg gctcacagcg
     1981 gcgatcgagc tggctgtggg cacccattcg gttctcagcg aagcggaggc gtctcgcgag
     2041 ctggctctgt tgtatcaggc catggggcgg aaccaggatg cgctgcgtct gctgagtgcg
     2101 gcgcaccgac tctttggccg gctggaggcc cgcctggact tggtggacgt ctcgggtcgg
     2161 gtgcagcggc tcgaagacac ctatctcgcg ctggtgcggg actggggcca atcgattgaa
     2221 tcagcggaca gctacacctt cgggcactgc gagcgggtgg ccaactacgc gctcgccgtc
     2281 gccgaagcgc tgggcatgga agaagcgcat cggaccaccg tacgaatcgg cgcgtacctg
     2341 cacgacgtgg gcaaggttcg ggtgccgcac gaaatcctga acaagcctgg accgctgtct
     2401 cgtgaggagt tcgagatcat caagctgcac cccctttggg gtgtagagct gctggctggg
     2461 gtagactttc cctgggacat caaaccgatc attcgctggc accatgagaa gtacgacggc
     2521 accggctacc cagatcggct ctgtggtgat gagatcccgc tgtccgcgca ggtgatctgc
     2581 attgtggacg tgtacgatgc gcttacaacg acccgaagct accgcgcgcc aatgagcccg
     2641 ggagcagcgg cggcgcaaat gcacgaatcc cgtcggtggt ggcgccccga cgtgtacgag
     2701 gccttccgtc gtgcggtacc cgacgtggcg gaagcggcct aaaaacccac tgctacacct
     2761 cgacgagccg cttggtgtaa acgaccagat cgtccccagg tgcgtaaaag tccgggatac
     2821 gggcggtcgc ctcgtagccc cgtgcctgat agaagcggcg ggtatcggcg tagtcggctc
     2881 gtcctgcggt ctcaaccacg atgagccggg ctcgtccgac aagccgccgt tccatctcgg
     2941 cgagcagcgc acttcctacc ccgacatgct gaccggctgg atcgaccgcc atccagtaga
     3001 ggtcgtacgt acccagcgtg cagggcgtgg ctccccagca aatccagcca acgagacggc
     3061 cctcgacatc cgcgccgagg gccgtgtagt ccggttcgct agcttgggca ggtcgagcac
     3121 cggctaccgc ggcatcgaac acctccaatg ccaccggcag ctcatccgaa cgaaagacgc
     3181 cgaccgcccg agtaattgct tcgattcggt cgcggtcggc ggcggagagg ctccggagtt
     3241 ccagcccgac gagcgtgtgg gtcaggcggc aggcaccccg cgcaccaggt cggccgcgaa
     3301 gcggctcggc tgcatccgag tcagcgcctc atcgacgatc tgatgcacca gctcgtcgta
     3361 actccagccg aaggcctgtc ccattcgggc caggccggca ttactggaaa gatcggggca
     3421 gggattgacc tcgaggacgt acggttgtcc atcctcggtg acgcgcaggt ctacccgtcc
     3481 atatccctca gctccagcca acagctccca ggcgcgccac gcaacgtggg tcaagcgctt
     3541 catcaggtcg ggcgcgatct cggccgggca gacgggttga gtacccagat cgtctgcact
     3601 tcccgtggtc cacttcgcgg cgtaggtcac gatgggccag gtgtccgctg gcatgctgtc
     3661 gaaccggatt tccgagaggg gcagcatggt cttgccaacg aaccctacgt tgatctcgcg
     3721 gccgggtacg tactcctgga tcatcacgtc ttcgaactgg tcggtcatct gcgcgacccg
     3781 cttgcgaagc gccttgcgag tcgtgcaaac cgcgttgcca tcgatgccga cgctggcgtc
     3841 ttccgctgcg ggcttcacaa tcgcggggag cggaaagtcg gcgggaatcg cgttgccctg
     3901 cgcgagcacg aaatgcggaa tcgggatgcc ggcgctctga agcagcgtgt tcgcgacgtg
     3961 cttccgatgg cacaccgtga cgggccaggc gcggcagccg gtgaagggca ggccggtcag
     4021 ctccacggtg ttgacgacca agtcttcgta cttggcgtgt ccgttgattc cttcgcagag
     4081 gttcacgatc acatccgcct tgcgcgcccg ggagagccaa cggaattcgc cgagccggac
     4141 ggggacgagc tccacgtcgt agtcgcggcg gcgaagcgac gttcgcacct cgtgcacgtt
     4201 gctgagcacg gcggcgacgt cctgcgggct ccactcaacg ctaccggcgt catagatgat
     4261 cgctgctctc atgaggctac ccctgccagg agtgaaccgc cgctgattcg gcgccaggcg
     4321 atgtctgcca cggtgcagat cagctcctcg tacgacatcc cggccgctgc tgcggccttc
     4381 gggaagcatg agttgtcccg cgggtcgggc aaaatgcccg gcagcggatt caactcaacg
     4441 accatcgggc gaccggccgc gtcgcagcgg agatcgatgc ggcaccaatc gcggcagtcc
     4501 agggcatggt acgcggcgag cgcggcctcg cgtactgcag tggcgagcga ctcgggaatc
     4561 cgcgcggggc actcgaagat atcgagcgga tgactgggcg tgtcccagag ccacttcgct
     4621 tcgtagccgt agatcggcgg ggctcctggg ggcaacgtgt cgaagcacat gccgacgatg
     4681 ggcaaacacc gtgcctcatc tccgttgccg agaatggcaa ccgtgaactc acggcccggg
     4741 agataggtct cgatcagcac cggctctcga taggttgcca ggagatagtt gacctgggtg
     4801 gtgagctcgg cccggctgtg gcacacgctc ttgctcgaga cgcctttgcc tgagccctcg
     4861 aaggccggct tcacgaagag tgggaagggc aggcgggtag cacgcgcctc cgcgacggtt
     4921 cgagccaagc ggaacggcgc cgtgggtaca ccgtagcgct ccagcatctc cttggcccgg
     4981 cccttgtgaa ggcagaggcc gagggtcagc gggtcggaag cgtgatacgg cacacccagg
     5041 aactcgcaga tggcggggac gtgactctct cgactcgggc cgaacaatcc ctcggcgatg
     5101 ttgaagacga agtcaggccg atagtcgagc agcttggcag ggaagtcttg atcggcttcg
     5161 aggcggatga cttcgcccag ggtcgcgagt gcgttctcca cggcgtcgat cgttgccggg
     5221 tcgtcccact cgatgtagag gtcaggttgc ggaaggtcgg ggcggagagc ccgactggga
     5281 ggttcatcag tggcggagga gtcgatcgct ggtccggcac cggcggccgg gacctcaggc
     5341 tttacgttga aagcgagacc gatccgaatt ggcagagtga ccctctacaa aaaaggccgt
     5401 ccgcgcgaga cgagccgtga cgagtgacct cctgacgatt ctaaagtaca gaaaacattt
     5461 ccgacgcaca ataggaaaac tgtgcgtgaa cggtgaacgg taaacggtga acgggagtcc
     5521 ggatgataag cggagcgtcc tgttcaccgt tcactgttca ccgttcacgt tcttcagacc
     5581 ttgtgaaacc cgacgct
//
LOCUS       DASRJQ010000528         3254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3303187, whole genome shotgun sequence.
ACCESSION   DASRJQ010000528 DASRJQ010000000
VERSION     DASRJQ010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3303187"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VFB89_12120"
     CDS             <1..322
                     /locus_tag="VFB89_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28099.1"
                     /translation="AGAFAELEKARLVVKLRHARDRIRNERGKCEGRKTRIERAKREN
                     DSKLVKALTEAVEMAKRLRRASPKTGERRSLRKISAELAAAGYLNERGRPFNPNSVKL
                     MLST"
     gene            complement(480..>3254)
                     /locus_tag="VFB89_12125"
     CDS             complement(480..>3254)
                     /locus_tag="VFB89_12125"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM28100.1"
                     /translation="VVVDNRSNDSAAEPAAVGETPLLAARLMNLASPGAVVISGSMRR
                     LLGRLFDYRQVPDAELQEAAEPVAACQVLSESGIASRFEALRSRRTRLIGREEEVELL
                     RRRWTQVKETGQGRVVLVSGEPGIGKSRLAATLEDTIETEPHACLRYFCSPHHTQSAL
                     HPVIAQLERAALIEREDSADVRLDKLEALLAPFCESLSQDVPLFALLLSIPGGERYLL
                     PSVTPQLIKERTMRALLTRLKHVAARQPVLMVFEDLHWIDPTSLELLSLVIDQMKGQR
                     VLLLATARPEFTPPWPSHRHISSVALSRLDRSEVGAVAAGITGGKTLPNDILDQIFAR
                     TDGVPLFIEELTTSLLETGLLRETANHYVLEGPLPLLAIPSTLHASLLARLDRLGMVK
                     DVVQMGAAIGREFRYELLAAVASLTRADLDAALERLTASGLISRRGTPPHATYTFKHA
                     LVQDAAYQSLVRSTRQQYHLRIADALEQQFPERAQSEPEVAAYHCAEAGMNNRAVRYL
                     RQAGLRAMARAANREATTHLEQALGALRRLPETRETTELTIDIRVDLRNALNPLGEWA
                     RMGEHLYEAEALAKRLGDQHRLGRIDTFMLIQCLVTGDYDEAVRFGQEALNIGRTLGD
                     RAIEVVATTYLGQTHAARGEFSDAVSLLERNVALEGDLRYERFGAPVIQSVRSGADLA
                     DVLSQLGRFDQAIGHAEAAVRIAEAADHPFTLFFALFDLGRAHLRRGDLPRATRVLER
                     SLDLCRTWQLVGGTPFVAAALGAAHALAGRVDEALPLVASAVDEFSSRQIQQWPAFIL
                     LCAGMTYLSAGKIDEASSHARAALALTRRLGARGSEAHTLCLAGDVASIGSTGDAEGY
                     YRKALALAETLGLRPLVAHCHLGLGKLYRRAGKRDEARQHLITATTTYREMDMRFWLE
                     KAGSEIKALH"
BASE COUNT          582 a         1030 c         1095 g          547 t
ORIGIN      
        1 cgccggtgcc ttcgccgagc tcgagaaggc gcgtttggtg gtcaagctcc gccatgcgcg
       61 cgaccgcatc cgcaacgagc ggggcaagtg cgagggtcgc aagacccgca tcgagcgcgc
      121 aaagcgggag aatgactcaa agctcgtgaa ggccctcaca gaggccgtgg agatggcaaa
      181 gcgcctccgc cgggcgtcgc ccaagaccgg agagcgcaga tcgctgcgaa agatcagcgc
      241 cgagctcgca gcggcaggct atctcaacga gcgcggccgg ccattcaatc ccaacagcgt
      301 caagttgatg ctctcaacgt gacacctgcg tgccggctcg gctcagccat ccgagctgta
      361 gcggctcaat ttgtccggac ttctcccata gctgacgaac gttccgcgac gcgattgacc
      421 ctgagcggaa gtccgggtcc ctcgcatttc cgcgacagcg agatctttgg atcaatggat
      481 cagtgcaacg ccttgatctc tgacccagct ttctctagcc agaaccgcat gtccatctcg
      541 cgatacgtag tcgtcgcggt gatgaggtgc tggcgggcct catcacgttt gcccgcgcgc
      601 cgatagagct tgccgaggcc gaggtggcag tgggcgacga gggggcgcag gccaagcgtc
      661 tcggcgagag caagcgcctt gcgatagtaa ccttcggcgt ccccggtgct gccgatcgag
      721 gccacgtcac cggcgaggca gagggtgtgg gcctcgcttc cccgagcccc cagccggcgg
      781 gtcagcgcca gtgctgctcg ggcgtggctt gaggcttcgt cgatcttgcc ggccgagagg
      841 taggtcatgc ccgcgcacag aagaatgaac gctggccatt gttgtatctg gcgactgctg
      901 aactcgtcga cggcgctcgc gaccagtggg agcgcctcgt caacccggcc ggcgagggcg
      961 tgggcggcgc cgagggccgc cgcgacgaac ggcgttccgc cgacgagctg ccacgttcgg
     1021 cagaggtcga gactccgctc gaggacccgg gtcgcgcgcg ggagatcgcc gcgacgcaga
     1081 tgggcgcggc cgagatcgaa caacgcgaag aacagtgtga agggatgatc ggccgcttca
     1141 gcgatccgca cggcggcctc ggcgtgccca atggcttggt cgaaccggcc gagctgggag
     1201 agcacgtcgg ccaggtctgc ccccgagcgc accgactgga ttacgggcgc cccgaagcgc
     1261 tcgtagcgta ggtcgccctc aagcgcgaca ttccgctcga gcaaggagac cgcatcgctg
     1321 aactcgcccc tggcggcgtg cgtctggccc agataggttg tggcgaccac ttcgatcgcg
     1381 cgatcgccga gggttcgtcc gatattcaga gcctcctgcc cgaatctgac ggcctcgtcg
     1441 tagtcgccag taaccaggca ctggatcagc atgaaggtgt cgatccgccc gagccggtgc
     1501 tggtcgccga gcctcttggc gagcgcttcc gcctcgtaga ggtgctcccc catgcgcgcc
     1561 cactcgccga gcgggttgag cgcgtttcgg aggtcgaccc ggatgtcgat cgtcagctcg
     1621 gtcgtctccc gggtctcggg gagacgacgg agggccccga gcgcctgctc caggtgggta
     1681 gtggcctccc ggttggccgc ccgcgccatg gcccgaagcc ccgcctggcg gaggtagcgg
     1741 accgcacggt tgttcatacc cgcttcggca caatgataag ctgcaacctc aggctcgctt
     1801 tgcgcacgtt cggggaattg ctgttccaac gcgtccgcaa ttcgcaggtg gtattgctga
     1861 cgtgtcgatc tgacaagtga ctggtaggcg gcatcctgca ccagagcgtg cttgaaggtg
     1921 taggtcgcgt gcggaggcgt tccgcgtcgg gaaatgagac cggaagcggt caggcgctct
     1981 agtgccgcgt cgaggtccgc ccgggtcaag gacgcaactg cggccagcag ctcatatctg
     2041 aactcacggc caatggcggc gcccatctgt accacatcct tgaccatgcc gagacggtcc
     2101 aagcgggcga ggagggaggc atggagcgtc gacggtatgg ccagtagcgg aagtggccct
     2161 tccagcacgt agtgatttgc ggtctcgcgc aaaagtccgg tctcgagcag agacgtcgtg
     2221 agctcctcga tgaagagcgg cacgccgtcg gtgcgcgcga aaatctggtc gaggatatcg
     2281 tttggcagtg tcttgccccc ggtgatgcct gccgcgacgg ccccgacctc cgagcgatcg
     2341 aggcggctca atgccacgga cgatatgtgc cgatggctcg gccacggcgg cgtgaactcc
     2401 gggcgtgcag tcgccagaag caagacccgt tgacctttca tttggtcgat gaccagcgac
     2461 aacagctcca gcgacgtcgg gtcgatccaa tgcagatcct cgaagaccat gagcaccggc
     2521 tggcgcgcag cgacgtgctt caggcgagta aggagcgctc tcattgtgcg ctctttgatc
     2581 agctggggag tgacgctggg tagcagataa cgctcaccac ccgggatcga gagcagcagg
     2641 gcgaacaggg gcacgtcctg cgagaggctt tcgcaaaagg gcgccagtag ggcttccagc
     2701 ttgtcgagcc tcacgtctgc gctgtcttca cgctcgataa gggcagcccg ctcgagttgg
     2761 gcaatgacgg gatgcagcgc actctgcgtg tgatgcggcg aacaaaagta acgcaagcac
     2821 gcatgcggct ccgtctcgat agtatcttcg agcgtcgcgg ccagccgcga cttgccgatg
     2881 ccgggctcac cggagacgag caccacccga ccctggcccg tttctttgac ctgtgtccaa
     2941 cggcgccgca ggagttcgac ctcctcctcg cggccaatga gccgggtgcg acgcgagcgc
     3001 aacgcttcga agcggcttgc gattccactt tcggacagga cctggcaggc tgctacgggt
     3061 tcggcggcct cctgcagctc ggcatcgggg acctggcgat aatcgaatag gcggccgagg
     3121 agacgtcgca tgctgcccga gatcacgacg gcgcccggcg acgcaaggtt catcaggcgc
     3181 gcggcaagga gcggagtctc gccgacagcc gccggttccg ctgccgagtc attacttcga
     3241 ttgtcaacca cgac
//
LOCUS       DASRJQ010000529         3329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2893941, whole genome shotgun sequence.
ACCESSION   DASRJQ010000529 DASRJQ010000000
VERSION     DASRJQ010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2893941"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..1143)
                     /locus_tag="VFB89_12130"
     CDS             complement(169..1143)
                     /locus_tag="VFB89_12130"
                     /inference="COORDINATES: protein motif:HMM:NF037816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase A-like domain-containing protein"
                     /protein_id="HZM28101.1"
                     /translation="MATSGVAKPMDPGVLAEGARPPTTRKDGGSQAIQSIMDLVWMTG
                     GGGVANAPGDEEEARRAPKSMTDSEHLLTLLTLGGAALAGGAVAGSLSRAGASDLLAK
                     AAGGATAGGVVRTGADVVEGRASSAGTYLRDMLIGAVAGGALAGESAAEAARLARLQG
                     KQPSLSGQGAEPVLSEPVPPRSILSQPGGLSSTEGRVVVVDGKAKPPTHPLSKHGPGV
                     KDESLLERLAKEPKTPEATRFGDRARMESAIGQTINANSSEIQSWLKKGAKGKLALTG
                     KPKLGKLGEGFKKMGDKVVRVGSHRKVRVVLASDGKGGYVIHTAYPVR"
     gene            1207..1392
                     /locus_tag="VFB89_12135"
     CDS             1207..1392
                     /locus_tag="VFB89_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28102.1"
                     /translation="MRKEREQGGGIALTDALIDIVGQAVTGLSRVALASVIMSGPANV
                     PIMVLWFAFLRHEIPSS"
     gene            1647..>3329
                     /locus_tag="VFB89_12140"
     CDS             1647..>3329
                     /locus_tag="VFB89_12140"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HZM28103.1"
                     /translation="MWTTKLCLAAALTALSVAGCTKKNPHKCNTEEPCTLVDAPYCDI
                     TGQFGGTPYRCIPDPGVDKLRVDPSSKDFGSVALGQSSSATTFTITNTGTAPTGSLAV
                     SMGGANLSSFAIGANTCAGQTLAANATCTVAVTFQPTAAGAAAGELSVTGTPGGTARA
                     ALAGNGLASGALAIAPATQDFGSVVAGQNSATTTFTITNPGAAATGALTVALSGGDSS
                     QFALTSDGCSTQTLSAGGSCNVVVRFNPTTAGTKSSSIMVTASPGGSTSAVLMGTGLM
                     AGALTISPTSRDYGPVPQGGMSAPFAFTVTNTGGATTGSLSSALTGTGSDQFSITTNG
                     CAGMTLASQATCMIAVRFDPTSAGSKNASLTVSGNPGGNAATALTGMGLSPATLSVMP
                     AAQNYGDVAVGNNSTATITVSNSGGVASGTPSVAITGADMALFTIASNTCVGALPPAG
                     SCTASVRFAPTSAGNKAAMLTASATPGGMAVASLNGNGLAPGNLAIAPTARDFGSVLQ
                     GQMSSALSFTVSNSGQTATSSLSVGLTGSGAAQFSVMTDTCSGQTLAPSASCM"
BASE COUNT          567 a         1150 c         1069 g          543 t
ORIGIN      
        1 ggccctggca gccgcgattc cgcggatgag caagccctcg atctccgact gatgcgcggg
       61 atcaccacag ctatcagcgg ccagagctcg tgcaccatcc tcgatcgtct catcgcccgt
      121 gtaggcggcg aggatgttcc agagttcctt tgtccaatcg gaatccattc agcgcaccgg
      181 atacgcagtg tggataacgt aaccgccttt gccgtctgac gccaaaacca cacgcacctt
      241 ccggtgcgaa ccaaccctta cgaccttgtc tcccatcttc ttgaacccct cgccgagctt
      301 gccaagcttc ggttttccgg tcagtgcgag ctttcccttg gcccccttct tgagccaaga
      361 ctgaatctcc gagctgttcg cattgatcgt ttggccgatc gccgactcca tcctcgcgcg
      421 gtcgccgaat cgggtagcct ccggcgtctt cggctccttc gcgaggcgtt ccagcaggct
      481 ctcgtccttg acgccgggtc cgtgcttgct gagtgggtga gtcggcggtt tcgctttgcc
      541 atcaacgacc accaccctgc cctcggtcga cgagagtcca ccgggttgtg acaggatgct
      601 cctcggcgga accggttctg acagcaccgg ctcagcaccc tgtcccgata gcgagggctg
      661 cttcccctgg agcctcgcga gccgggcggc ctcagccgca gattcacccg cgagcgcgcc
      721 gccagcgacc gctccgatca gcatgtcgcg caggtaggtt ccagccgagc tcgctcggcc
      781 ttccaccaca tcggctcccg tgcgcaccac tccgccagca gtcgcgcctc cagcagcctt
      841 cgcaagcagg tctgacgctc cggctcgtga cagactgccc gccacggccc ccccagccaa
      901 tgccgccccg ccgagcgtga gcagcgtcag caagtgctcg ctgtcggtca tgctctttgg
      961 tgcacgccgc gcttcctcct cgtcgcccgg cgcattcgcg acgccaccac cacccgtcat
     1021 ccagacgagg tccatgatgg actgaatcgc ctggctgccg ccgtccttcc gggtcgtcgg
     1081 gggacgggcg ccttcggcga ggactcccgg atccatcggc ttcgcgacgc cgctcgtcgc
     1141 catccgaacc acgtccgcgg gcagcggcgg caggcctcga ggagcgcgac gctcgtcttc
     1201 ggccagatga gaaaagagcg cgaacagggc ggtgggatcg ccctgaccga tgctctcatc
     1261 gacatcgtag gtcaggccgt caccgggctc agtcgggtcg ctctcgcgag tgtgatcatg
     1321 agcggccccg ccaacgtgcc gatcatggtg ctctggttcg cgttcttgag gcacgagata
     1381 ccttcgagtt gaggtcgttc ggaatcttca tcggcgaacg tcaccggaag ttgcgtgatc
     1441 tcgagccgga gagcagagag tggcggcctc acttttgccg caccacgcaa gatcaggcac
     1501 ttggggccat ggcgggaaca gggagctgct tgttcgcagc ggtgagagcg gatagggatg
     1561 ctggtggtcg ggcgaaacgc tcgcagggac gcaacaccag gccgggtccg tcgatacagg
     1621 gatcggacct gacagggaga cagaccatgt ggacgacgaa gttgtgcctg gcggcggcgc
     1681 tgacggcctt gagcgtcgcg ggctgcacca agaagaaccc gcacaaatgc aacaccgagg
     1741 agccatgcac gctggtggat gcgccctact gcgacatcac gggacagttc ggcggcaccc
     1801 cctatcgctg tatcccggat ccgggtgtgg acaagctcag ggtcgacccg tcgagcaaag
     1861 acttcggatc ggtggcgctg gggcaatcca gcagcgcaac gacgttcacc atcaccaaca
     1921 cggggacggc gccgacggga agtctcgcgg tctccatggg cggcgccaac ctgtcgagct
     1981 tcgcgatcgg cgccaacacc tgcgcgggcc agacgctcgc ggccaacgcg acctgcacgg
     2041 tggcggtcac gttccagccc acggccgctg gagcggctgc aggtgagctc agcgtgaccg
     2101 gaacgccagg cggaaccgca cgcgccgccc tggcaggcaa tggcctggca tctggtgcgc
     2161 tcgcaatcgc gccagccacg caggacttcg gctcggtggt cgcgggccag aacagcgcga
     2221 cgacgacctt cacgatcacg aaccccggcg cagccgccac gggtgccctg acggtcgcgc
     2281 tgagcggagg agactcgagc cagtttgccc tcaccagcga cggctgctct acgcagactc
     2341 tgagcgccgg gggcagctgc aacgtggtcg tccgtttcaa cccgacgaca gctggaacca
     2401 agagcagcag catcatggtg acggcctcgc cgggcgggtc cacctcggcc gtcctgatgg
     2461 gcaccggcct gatggccggg gcgctcacga tctctcccac cagccgagac tatggacccg
     2521 tgccccaggg cggcatgagc gcgcccttcg ccttcacagt cacgaacacg gggggcgcga
     2581 ccacgggatc gctgtctagc gcgttgaccg gcaccggatc ggatcagttc tccatcacta
     2641 ccaacggctg tgcggggatg acgctggcga gccaagcgac gtgcatgatc gcagtccgat
     2701 tcgatccgac cagcgccggc tcgaagaacg ccagcctgac ggtgtcgggg aatccgggcg
     2761 gcaacgcagc gaccgctctc accggcatgg gtctctcgcc ggcgaccctc tccgtgatgc
     2821 cggcggcgca gaactatggc gatgtcgcag tcggcaacaa ctcgaccgcg accatcacgg
     2881 tcagcaacag cggcggcgtc gcgtccggaa ctccgagcgt ggcgatcacg ggcgcagaca
     2941 tggcgctgtt cacgatcgcc tcgaacactt gcgtcggcgc gctgcccccc gcgggcagct
     3001 gcacggcctc ggtgcgcttc gcgccgacca gcgcagggaa caaggcggcc atgttgaccg
     3061 cgagcgcgac ccccggcggg atggcggtgg caagtctgaa cggcaacggg ctggctccgg
     3121 gcaacctcgc gatcgctccg accgctcgcg attttggctc ggtcctgcag gggcagatgt
     3181 cgtcggcgct atcgttcacg gtgagcaaca gcggccagac cgcaaccagc agcctgtccg
     3241 tcggcctgac gggcagcggc gcagcgcagt tctcggtgat gacggatact tgctcgggtc
     3301 aaacgctcgc gccgagcgcg agctgcatg
//
LOCUS       DASRJQ010000530         2003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2039625, whole genome shotgun sequence.
ACCESSION   DASRJQ010000530 DASRJQ010000000
VERSION     DASRJQ010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2039625"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1287)
                     /gene="ltrA"
                     /locus_tag="VFB89_12145"
     CDS             complement(40..1287)
                     /gene="ltrA"
                     /locus_tag="VFB89_12145"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HZM28104.1"
                     /translation="MRRENIAAAHARVVQNGGAPGVDGMTVDDLLPYCRKHWTRIREE
                     LLSGRYKPQAVRRVEIPKPDGKGTRMLGIPTVLDRMIQQALHQILTPIFDPTFSDGSF
                     GFRPGRSTHGAVRRAREHMAAGYRWVVDLDLEKFFDRVNHDVLMARVARRVKDKRVLL
                     LIRRYLQAGMMEGGLVSPRMEGTPQGGPLSPLLSNVLLDELDKELERRGHRFVRYADD
                     SNVYVQSEAAGKRVMASLERFLRERLRLKINREKSAVARPWKRKFLGYTVTWHRDPKL
                     KIALQSLQRLKAKLRSILRRGRGRNLLGVIEELNPVIRGWVAYFRLAEVKVSFEDLDA
                     WVRRKLRCILWRQWKKPRTRFKKLRQLGLDVDRAKAGASNGRGSWWNAGASHMNQAIS
                     TGLLRRWGLVSFLNEYWRLKSAS"
BASE COUNT          305 a          706 c          573 g          419 t
ORIGIN      
        1 ctcccacacc accgtacgta cggttccgta tacggcggtt cacgaggcgc tcttgagtcg
       61 ccagtactcg ttgaggaagc tcacgagccc ccatcgtcgc agcaatccgg tcgagatcgc
      121 ctggttcatg tggctcgctc cggcattcca ccaggagcct cggccgttag acgccccggc
      181 cttggcgcgg tccacatcga gcccaagctg gcggagtttc ttgaagcggg tgcggggttt
      241 cttccactgt cgccacaaga tgcagcgtag tttccgacgc acccatgcat ccagatcttc
      301 gaagctcacc ttgacctcgg ccagccggaa gtacgcgacc cagccccgaa tcacagggtt
      361 gagttcctcg atgacgccga ggaggtttcg acctcgtcct cggcgcagga tcgaccgaag
      421 cttcgccttg agtcgttgca gtgactgcaa agcgatcttc agcttgggat cccggtgcca
      481 cgtcacggtg tagcctagaa acttgcgctt ccagggtcgt gccaccgcgc tcttttctcg
      541 gttgatcttc agccggagtc gttcgcgcag aaaacgctcc agcgatgcca tcacccgttt
      601 gcctgccgcc tcagactgca cgtatacgtt gctgtcatcg gcatagcgga caaaccggtg
      661 tccccgtcgc tccagctctt tgtccagctc gtcgagcagg acattgctga gtaacggtga
      721 aagcggaccc ccctggggtg tcccttccat tcggggcgac actagcccgc cttccatcat
      781 ccccgcttgt aaatagcggc gaatcaggag cagcactcgt ttgtccttca cccgtctcgc
      841 cacgcgcgcc atcagcacgt cgtggttcac acggtcgaag aatttctcga ggtccagatc
      901 gaccacccag cggtatcccg ccgccatgtg ctcgcgagcg cgccgcactg cgccgtgcgt
      961 gctgcggccc gggcggaaac cgaagctccc gtccgagaac gtcgggtcga agatgggtgt
     1021 caagatctgg tgcagggcct gttggatcat ccggtccagc accgtcggga tacccagcat
     1081 ccgcgtccct ttcccgtccg gcttcgggat ttccacccgc cggacggcct ggggtttgta
     1141 gcggccgctg agcagttcct cccggatgcg cgtccagtgc ttccgacagt agggcagaag
     1201 gtcgtccacg gtcatcccgt cgactcccgg tgccccgccg ttctgcacca cacgcgcgtg
     1261 tgctgccgcg atgttctcgc gacgcactac ttcctccatg agcagcaatg acccctcgcc
     1321 ggtttgctct ctacgagccg ggcgcgcttg gcgcgcgact ctcggaccct gggcggttcc
     1381 gccgcttgcc ttccgaggtc gctccggcat ctcagccttc ttgcctgcgt ctacttcgcg
     1441 cctcgatcgc atcgaaccgg ttcggctcct cgcgttcggc cctcaccgcc gtgcgttgcg
     1501 gcttgttggc ctcggctgac ttctgccctt ccttcagaac gcctctcgac gtccctagct
     1561 cgaagcagtc gggcagatct cccggggtaa gacgcatgac cttcgcccca tacacccgcc
     1621 gcatctacgc ccgcccagtc cgggtgactt cgggcttcgg gttttcatgg ccccctcgcc
     1681 cgccaggcgg tcgcctccta tgcggttcgt gttcctcagg ccggggcttt gcttacagct
     1741 tccttcacac cctgcctcgc ggcagcgcgc ttgctgttcg gctaggggtt cccgtcacca
     1801 aggtccccag aggactccca cctcctggtc acttcccgtc cggctttcgc ctcgcggtta
     1861 tcgctgcccc tttcggggcg tctgcgccat gcccggcgca ccatgaaagg agggaaggac
     1921 agcaagatcc ttccccagcg agtgtgaaac cgtgccgcca tcggatgaga tggcagtggg
     1981 taccggagcg ccgggcatca tga
//
LOCUS       DASRJQ010000531         5703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2741691, whole genome shotgun sequence.
ACCESSION   DASRJQ010000531 DASRJQ010000000
VERSION     DASRJQ010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5703
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2741691"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1931
                     /locus_tag="VFB89_12150"
     CDS             <1..1931
                     /locus_tag="VFB89_12150"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HZM28105.1"
                     /translation="LETLMRDVVRTRGAAPLEATPAPGVTSPQPDAVLSSKASPAAHF
                     KAEPGGGTPPPLPRALDRTAAFESNARRKANFLRSEEWIGQRGLLAIGVLALILAAGY
                     LLKLSFDRGWISPFVRCVGGAVAGGIVGALGWSLLGRYRTYGASLIGCGAAIIYLAVW
                     AAAKLYGLLPPATGIGALALVSLALAAVAFAINVEALGTTAALGAFIAPVLLGKRGNP
                     DALLIYLTALGVALGWVAGHKRWRTTAALVALSFFGLGWAGVADSRHPIGALGYTMLG
                     GAGGIYVGLRERWWETRFLAFWGGWALLATVHDKLSPTWPTLVAGILLAAPVWWHALR
                     RKTWLPIRLMASVPGEGLTVGEALYFFSTPFLVAWAAPTCAPDFFAEHAGLAALVVAV
                     PYLLSGYATPRPPFALVGVTALATAVLQRWSGFEGPAILFGLAGLWAGLDHVLQRHDG
                     RWYSVGTLIVALVQLLGDVAGRRSFDDAAFVGPWALVLWLGLAVTTVLAAGLWRREPR
                     SPAAELVPRLLWLTAGSIALFGVTAEITRFYRLREGPVEAAHLASGLAVSAWWLVFAA
                     ALVVLGLRRSLRPVRLAGLAVAGLAVFKVLVFDLSSLDALYRVGSVFILGLVSLLLAY
                     LYHRQARSPDTGRRPLEL"
     gene            1977..3704
                     /locus_tag="VFB89_12155"
     CDS             1977..3704
                     /locus_tag="VFB89_12155"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HZM28106.1"
                     /translation="MIGSALPSSPEAFAKATWDDIVPYYDELAERSLEPDSMEAWLRA
                     WSTLEELLTEAAARAMIAYTIDTGDAEKEANHLRFSSDILPKAEERSVALARRLVESG
                     YERPDLATTIQRFRTSIEIFREANVTLFAEVEQHCATYQRITGSMTAEWEGEQRPLPQ
                     LLPFLKSPDRRIRERAWRASTAPYLAQRDAMSKLFDQMYTLRQQAARNAGFANFRDYI
                     FPAKFRFDYTPADCERFHEAVERTVVPAIGRLLAYRRERLGVDTLRPWDLQVDPYRDE
                     PIRPFHTADELVTRARRVFERVDPVLGSEFQTMIDHRLLDLDSRKGKAPGGYCETLHF
                     QGRPFIFMNAVGLVDDVNTLMHEGGHAFHAFASHQQPFIWQRHPGSEAAELASMSMEL
                     LAAPHLATPTGYFSRDEARSAALEHLEDILIALGHIASIDAFQHWIYTSGEGGDQEAR
                     DRAWLRIRARFERELDWEGLEEHRVARWYRQLHIFLYPFYYIEYGIAQLGALQVWRNS
                     LADPVGAVVHYRRALALGATRPLPDIYRAAGAELSFDTGLMSELVALVESRIQALRAE
                     LPAPGQRSA"
     gene            3761..3979
                     /gene="infA"
                     /locus_tag="VFB89_12160"
     CDS             3761..3979
                     /gene="infA"
                     /locus_tag="VFB89_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HZM28107.1"
                     /translation="MAKEEGIEMEGVVTEVLPDRNYRVLLENGHEVLAYAAGKMSKFK
                     IRVLEGDRVSVVLSPYDLTRGRVIYRHK"
     gene            complement(4047..4259)
                     /locus_tag="VFB89_12165"
     CDS             complement(4047..4259)
                     /locus_tag="VFB89_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HZM28108.1"
                     /translation="MARITGTVKWFNDAKGFGFITPENGDKDCFVHHTAIKMDGFRSL
                     AEGEKVEFDIVQGAKGPAAENVTKAK"
     gene            complement(4488..>5703)
                     /locus_tag="VFB89_12170"
     CDS             complement(4488..>5703)
                     /locus_tag="VFB89_12170"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZM28109.1"
                     /translation="FMAPEVRGGAPGDPISDVYTAGGVLYYAITGQEPPLDTREIRRP
                     TELRPNCPRALERVIIRALQPVPDDRYQTAAEMLEDFASEAGTFEVPSSTPATGVPAL
                     LDNEDKARWEKRLRRALGDDYELLAMLGQGGFGRVYRVRDLHLEREVALKVLHPLLTQ
                     EPAVVERFRREAQLAARLTHPHIVNIYDIAGRSGLIWYTMELIQGPSLAQLVEREGPM
                     SLDRVLRLLREALSALAHAHGSGLVHRDIKPENMLLEPDGSLRITDFGLALALRGAGR
                     FGGATSQSGTPQFASPEQLLGERVDQRSDLYSLAAVATYALLGTAPFPGITPEQVLAR
                     QTTNQFPVTEGVREDVPEELWAVLERATRAEVEARFPSAAEFLQAVNRAAGTPRRSGG
                     DLARVAARWLKI"
BASE COUNT          886 a         1838 c         1896 g         1083 t
ORIGIN      
        1 tgctggaaac gttgatgcgt gatgtggtgc gcacgcgagg ggcggccccg ctcgaggcca
       61 cgccggcgcc gggcgtcacg tcgccacagc ccgacgcggt tctctcgtcg aaggcttctc
      121 cggcagccca cttcaaagcg gagcccggag ggggcactcc tcccccgctg ccgcgcgccc
      181 tcgaccggac cgcggcgttc gagagcaacg cccggcggaa ggccaacttt ctccgttcgg
      241 aggagtggat cgggcagcgc ggcctgcttg cgatcggcgt cctcgcgctg attctcgcag
      301 cggggtatct cctcaaactc tcgttcgacc ggggctggat ttcgccgttc gtccgctgtg
      361 tcggcggtgc ggtggccggc ggtatcgttg gcgcgctcgg ctggagcttg ctcgggcgct
      421 accgcaccta cggcgcttcg ctcatcgggt gcggcgctgc aatcatatat ctcgcggtct
      481 gggcggcggc aaaattgtat gggttgcttc cgccggccac cggcatcggc gcgctggcac
      541 tcgtgtcgct cgcgctcgcg gccgttgcct tcgccatcaa tgtcgaggcg ctgggcacga
      601 ccgcagcgct cggcgcgttc atcgctccgg tcctgctcgg caagcgaggc aatcccgacg
      661 cgctgctcat ttatctaacg gcccttggtg ttgcgctcgg atgggtcgcg gggcacaagc
      721 gctggcggac cacggcggct cttgtggcgt tgagcttctt cgggctgggc tgggcgggcg
      781 tcgccgactc acgacacccc atcggcgcgc tgggatacac gatgctgggg ggtgcgggcg
      841 ggatctacgt gggactgcgg gagcgttggt gggaaacccg gttcctcgcg ttctggggag
      901 gctgggctct tctggccacg gtccacgaca aactcagtcc cacctggccc acgctggtcg
      961 cgggcattct tctggccgcg cccgtctggt ggcacgcgct ccgccggaaa acctggcttc
     1021 caatcaggct catggcgagt gtgcccggcg aggggctaac ggtcggcgaa gcgctctact
     1081 tcttctcaac cccatttctc gtcgcatggg ccgccccgac ctgcgctccc gacttcttcg
     1141 cggagcacgc cggacttgcc gccttggtgg tagccgtccc ttatctcctg agtggatatg
     1201 ccacgccgcg gccgcctttc gcgcttgtcg gcgtcaccgc ccttgcaacg gcggtgcttc
     1261 aacgctggtc gggcttcgaa ggtcccgcaa tcctgtttgg cctggcaggc ctttgggcgg
     1321 gactggatca cgtccttcaa cgacatgacg ggcggtggta ttcggtaggc acgctgatcg
     1381 tcgcgctggt ccagctgctg ggtgacgtcg ccggccggcg cagtttcgat gacgctgcgt
     1441 tcgtgggacc gtgggcgctg gtgctgtggc ttgggctggc cgtgacgacc gtgctcgccg
     1501 ccggtctctg gcgccgcgag ccgcgaagcc cagccgcgga gctggtgccg cgcctgctgt
     1561 ggctgacggc gggctcaatc gcgctgttcg gggtgaccgc cgagattact cgcttttatc
     1621 gactcagaga gggtccggtg gaggcagctc atctcgccag cgggttggcg gtgagtgcgt
     1681 ggtggctcgt cttcgccgcg gctctcgtcg tgctcggcct gcggcgttcg ttgcgcccgg
     1741 tgcgactggc cggccttgcg gtagctgggc ttgcggtatt caaggtgctg gtcttcgatc
     1801 tttcgtcgct cgatgcgctg tatcgggtgg gctcggtatt tattcttggc ctggtgtccc
     1861 tccttctcgc ttacctctat catcgtcagg cgcgcagtcc cgataccggg agacgcccgc
     1921 ttgaattgtg agcgggatgg cgccatcatc tccgcttccg taacgcgagt cagcgtgtga
     1981 ttggttcggc cctcccttcg tcgccggaag cctttgccaa ggccacgtgg gacgacattg
     2041 tgccttacta tgacgagttg gccgagcgga gcctcgagcc cgacagcatg gaagcgtggc
     2101 tccgtgcgtg gtccacactc gaagagctgc tgacggaagc ggccgcccgt gccatgattg
     2161 catacaccat cgacacgggc gatgccgaga aagaagcgaa ccacctgcgc ttctcgagcg
     2221 acattctgcc caaggccgaa gagcgaagcg tcgcactggc tcggcgtctg gtcgagtcgg
     2281 gatacgagcg acccgacctg gccacgacga ttcagcgctt ccgcacctcc atcgagatct
     2341 ttcgggaggc caacgtaacg ctgttcgcag aagtcgagca gcactgcgcg acgtatcagc
     2401 ggatcacggg ttcgatgacc gcggagtggg aaggagagca gcgcccgttg ccgcagctgc
     2461 tgccgtttct caagagtccg gaccgcagga tacgggagcg agcctggcgc gccagcacgg
     2521 cgccatatct ggcgcagcgg gacgcgatgt ccaagctgtt cgaccagatg tacaccttgc
     2581 gccagcaggc agcgcgcaac gccggcttcg cgaatttccg ggactacatt ttccccgcca
     2641 agttccgctt cgactacacg ccggccgact gcgagcgctt tcacgaggca gtagagcgca
     2701 ccgtcgttcc ggcgatagga cggctgctgg cgtatcggcg cgagcgactt ggcgtggaca
     2761 cgctgcgccc ttgggacctt caggtagatc cgtaccgcga cgagccgatc cgtccgtttc
     2821 acacggccga cgagctggtg acccgcgctc gccgggtttt cgagcgggtg gatccggtcc
     2881 tcggctcaga gttccagacg atgatcgacc atcgcctgct cgacctcgac agccggaagg
     2941 ggaaagcgcc gggcggttat tgcgagacac tgcactttca gggacggccg ttcatcttca
     3001 tgaacgcggt cgggctggtg gatgacgtga acaccctgat gcatgaaggg ggtcacgcgt
     3061 ttcatgcgtt tgcctcccac cagcagccgt tcatctggca gcgacacccg ggctccgaag
     3121 cggcggagct ggcctcgatg tccatggagt tgctggcggc gccgcacctg gccacgccca
     3181 ccgggtactt ctcgcgagac gaggcgcggt cggcggcgct cgagcacctc gaggacatcc
     3241 tgatcgcgct cggtcacatt gcgtcgatcg acgcgtttca gcactggatc tataccagtg
     3301 gtgagggggg cgaccaagag gcgcgcgacc gcgcctggct caggattcgc gcccgattcg
     3361 agcgagagct cgactgggaa ggattggagg agcaccgggt cgctcgctgg tatcgtcaac
     3421 tgcacatctt tctctatccg ttttactaca tcgagtacgg catcgctcaa ctcggcgcac
     3481 tccaggtctg gcgcaacagc ctggcggatc ccgtcggagc cgtcgttcac taccgccgag
     3541 cgttggctct cggtgccacc cggccgctgc ccgacattta tcgcgccgcc ggtgcggagc
     3601 tgtcgttcga caccgggctc atgagcgagc tggttgcgct ggtagagtcg cggattcagg
     3661 cccttcgcgc agagctgcct gctccaggcc agcggtccgc ctgaggttgc ttcccggccg
     3721 cggagcggca agattccccc gtcctcaggg gagctagcgg atggcgaagg aagaaggcat
     3781 cgagatggaa ggtgtcgtga ccgaggtgct gcccgaccgc aattatcggg tcctgctcga
     3841 gaatggtcac gaggttctcg cgtacgcggc cggtaagatg agcaagttca agatccgcgt
     3901 actcgagggc gatcgggtga gtgtcgtact ctccccctat gacctcaccc gggggcgggt
     3961 catctatcgc cacaagtaga cggaaacgat gcgctgaaaa acaacgggcc gctcaatcga
     4021 gcggcccgcc gtgttgttgg acgcgcttac ttagccttgg tgacgttctc agcggcaggc
     4081 cctttcgcac cctgcacgat gtcgaactcg actttctctc cctccgcgag gctgcggaat
     4141 ccgtccattt tgatcgcggt gtgatggacg aaacagtcct tgtccccgtt ctccggagta
     4201 atgaatccga agcccttcgc gtcgttgaac cacttgaccg tgccggtaat gcgagccatt
     4261 gcgaccactc cgtgttggtt gactgcctcg gcggttcgga gaggtccgtg gcgccgagct
     4321 tttgtgagcg caggcctgtc ctgcgtcaca aaaagacgaa aagacccgag ggcgatcagc
     4381 ccgggtcttc aaagcattgt ggtttagtcg ttcgctgaga ccaaagaaaa ccaagccgga
     4441 aattcgcgct gttaaatatg tatccgggtc gcccttcgtc aagggcgtca tattttaagc
     4501 cagcgcgcag cgactcgcgc caggtcgcct cccgaccgac gcggagtacc cgccgcccga
     4561 ttcacggcct gaagaaactc cgccgctgag ggaaaccggg cctccacctc ggcccgcgtg
     4621 gcccgttcca gcacggccca cagttcctcc ggcacgtcct cgcggacccc ctccgtcacg
     4681 gggaactgat tggtggtctg gcgcgccagg acttgctcag gcgtgatccc agggaaggga
     4741 gcagtgccca gcagcgcgta cgtagcgacc gcggccaggc tgtagaggtc ggaccgctga
     4801 tcgactcgct ccccaagcag ctgctccggg ctcgcgaact ggggtgtgcc gctctggctg
     4861 gtggcgccgc cgaagcgtcc ggctccgcgg agtgccagcg ccaacccaaa gtccgtgatg
     4921 cggagactac cgtcgggctc gagcagcatg ttttccggct tgatgtcgcg gtgcaccagc
     4981 ccggagccgt gcgcgtgtgc cagcgccgac agtgcctccc gcagcaggcg gagcacgcga
     5041 tccaacgaca tcgggccctc gcgctccacc agctgagcca ggctcggacc ctggatcagc
     5101 tccatggtgt accagatgag cccggagcgc ccggcaatgt cgtagatgtt cacgatgtgg
     5161 gggtgggtga gccgggcagc gagctgggct tcgcgacgga atcgctcgac tacggccggc
     5221 tcctgcgtga gcagcgggtg caggactttg agcgcgacct cgcgctcgag atggagatcg
     5281 cgcacgcggt atacccggcc gaaaccgccc tgacccagca ttgccagcaa ctcgtaatca
     5341 tcgcccaacg cgcgacgcag acgtttttcc cagcgcgcct tgtcctcgtt gtccagcaag
     5401 gcaggtacgc cggtggccgg cgtcgaggat gggacttcga acgtgccggc ctccgacgca
     5461 aagtcttcca gcatctccgc cgcggtttga tagcggtcat ccggtacggg ctgcagagcg
     5521 cggataatga cgcgctcgag cgcgcgcggg cagttcggcc gcagctcggt gggtcgacga
     5581 atctctcggg tatcgagcgg cggctcctgg ccggtgatcg cgtagtacag cacaccgccg
     5641 gccgtataga cgtcgctgat cggatcgccg ggtgcgccgc cgcgcacctc aggcgccatg
     5701 aat
//
LOCUS       DASRJQ010000532         6678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_635578, whole genome shotgun sequence.
ACCESSION   DASRJQ010000532 DASRJQ010000000
VERSION     DASRJQ010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6678
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635578"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1383
                     /locus_tag="VFB89_12175"
     CDS             <1..1383
                     /locus_tag="VFB89_12175"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HZM28110.1"
                     /translation="GACNDLTVGDLNAPGIEETVNNPTRVGVLNLATGLQIGSRFGVG
                     QQNGYVAQLGIVGEEIYNFDPADPRFITEMVYGPLDGGSPAFGGNLFQPYANIRTANI
                     MLAAMDNLSSTPPAGMSPVEKEATLGYAQTMIAYDLLKALNTRWDNGIPIDVNIDPSG
                     PPAPIVDTSVAFTHIQNLLDSGATHLTTVGDASFPFQMSAGFSGFDRTLTFRKFNRAL
                     RARVAVYRNDFARALTILVPDSTFIDDGSTLTTGVYHTFTTAPGDSTNNLFDPNARAL
                     LAHPSLETDAQLQAGGAPDQRFLNKTTLLSSAHTSSGLSSNRALNVYAGPSAPVPIIR
                     NEELILLRAEANIGINNLAGAIPDINTARAAGGLTPLVAFASHDEALAELLYNKRYSL
                     LFEGGHRWIDMRHYGLLTSDPHPPFTTLQKEVTPSGTRHFFNRFPFPTNECLARTTQP
                     ARGCTLEPGS"
     gene            complement(1462..3327)
                     /locus_tag="VFB89_12180"
     CDS             complement(1462..3327)
                     /locus_tag="VFB89_12180"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZM28111.1"
                     /translation="MIASRTPLLAGIALLAGASASTAQQAGKPKTTAPARSTPTVPRS
                     APISNIRYDVTFDSATAVRRTIRVVMSFDVAAGKDPVLLSVPAWTPGAYEISSFARWI
                     SRFSASSSDDRPLAWNKADPDTWRIRTTGPGRVMVRFDYLADTLDNAMAWSRPDFLLF
                     NGTNIFPYPEGRGFDFPATVTVKTQPDWLVATGMKSAGAAFTWSEGNYHDLVDMPFFV
                     GRFDFDSMVVDGKTTRLASYPRGILKGETRAALWDQIAKAIPAMAAVFQEVPWNNYTV
                     MTIFDSSYQGGSALEHQSSHVGVYAPMMLGTPLLASITAHEIFHAWNVKRLRPADMVP
                     YSYDRVEPTPWLWVSEGITDYYADLALVRGKIVDSTIFMGLTAEKIQTVAETAPTALQ
                     DASLSTWIHPTDGSGYLYYPKGSLAGLLLDIVIRDASDNRRSLDDVMRELYRTTYKSG
                     RGFTAADWWGAVSRASAGKSFADFDARYIDGRDPYPYDAMLKLAGMRLASDTIREPRL
                     GVAGAPDSAGVKVTAIQPGGAAETAGVHAGDILIALGDVAITDPNFGPAYRARFKDAE
                     GQDFPIKVRRGADTLTLTGKVRLLEQVNAHVELDPNPSPKAVRIRQGILTGTTEK"
     gene            complement(3354..4652)
                     /locus_tag="VFB89_12185"
     CDS             complement(3354..4652)
                     /locus_tag="VFB89_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HZM28112.1"
                     /translation="MLTAVRRISLTGWIFVGMAAGILIGWLAPQEFALGLKPLSSTFL
                     RMIKSVVVPIIFGTLVIGIAGHGDDLKRIGRLAIKSIGYFWVMTTIALAIGLLAGNLV
                     KPGVGVRLPPPDPHAALPQPAPTTFAEFLEHVVPRSVVEAAANGEVLQVVFWATLFAI
                     ALTQVKGRPKEAMLGFAEGLMEVMFKFVAIVMKFAPIGVGAAMAVAVSHGGLGVLINL
                     GKLVLTLYGALFVFAFVALLPAALIARIDILRFARTVKDPALIAFTTTSSDAALPLAM
                     QRMVEFGVPKRIVAFVMPTGYSFNLDGSTLYLGVASLFVAQAAGVHLSLGQQLFMMLT
                     LLITSKGIAAVPRASLVVLAGTLTKFGLPLEGIAVILGVDEVMDMARTAVNLVGNCLA
                     TAVVGRWEGELASAPEAARIEARATGPAPELSRLSTPPSV"
     gene            complement(4743..5861)
                     /gene="tal"
                     /locus_tag="VFB89_12190"
     CDS             complement(4743..5861)
                     /gene="tal"
                     /locus_tag="VFB89_12190"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HZM28113.1"
                     /translation="MTTNPLVRLGELGQSPWYDFITRDLLASGQLTRLIADDGLLGMT
                     TNPTIFEKAVAGSDLYDEDIRQLTSRGRSSMEVFEALAVADVREACDAFLPVYQRAGG
                     QDGLVSIEVSPALARDTAATVVEAERLWRTVGRPNAMIKIPGTVEGLPAVTQCIAEGI
                     NVNITLLFSVERYEGVIAAYQAGLEARLARGLPVDWIASVASFFVSRVDTKLDPMLDR
                     IGDPSRLRGKIAIANACLAYRAFEKRRAESRWSALAERGARPQRPLWASTSTKDPRYS
                     EVHYVEALIAPNTVNTLPPETFAAYRDHGKPEVRIQTGIAAVDSQMASLAKLGLDFPS
                     LTRELEDEGVTKFAASYGAALATLEHKASALAGAAPAR"
     gene            complement(5858..>6678)
                     /locus_tag="VFB89_12195"
     CDS             complement(5858..>6678)
                     /locus_tag="VFB89_12195"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HZM28114.1"
                     /translation="GPSSTGRQIHWGVREHVMGAALNGIAAHGGLRPFGSTFLVFTDY
                     MKPSIRLAAIMRLPVIYVGTHDSIGVGEDGPTHQPIEHLAMLRSIPGMTVIRPADATE
                     TVEAWRVAIAHRSGPVALVLTRQKLPVLDRATLAPAAGVQHGGYVLWDSPGGPEAILM
                     ATGSEVHVALAAAQQLMAEGIRGRVVSLPSWELFEAQPREYRDSVLPPTIRARVGIEA
                     ASPFGWLRYTTDAGEMLALDHFGASAPAERLFSEFHFTPDHAAAAVRRVLGRTA"
BASE COUNT         1263 a         2141 c         2140 g         1134 t
ORIGIN      
        1 ggagcgtgta acgacctgac tgtcggcgat ctgaatgccc cgggcataga ggagacggta
       61 aacaatccca cccgggtggg tgtcctcaac ctcgcgaccg gcctccagat cgggagccgg
      121 ttcggcgtcg gccagcagaa cggctacgtc gcccagctcg gaatcgtcgg cgaggagatt
      181 tacaacttcg atccggccga tccgcggttc attaccgaga tggtgtacgg accgctggac
      241 ggaggcagcc cggcgttcgg cggcaacctg ttccagcctt acgcgaacat caggaccgcc
      301 aacatcatgc ttgcggcaat ggacaatctg tcgagtaccc ctcccgccgg catgtcaccc
      361 gtcgagaagg aggcgacgct ggggtacgcc cagacgatga tcgcgtacga tctcctgaaa
      421 gccttgaaca ccaggtggga caacggaatt ccgatcgatg tgaacatcga cccctcggga
      481 ccgccggctc cgatcgtaga cactagcgtg gcgttcactc acatccagaa cctgctggat
      541 tccggcgcga ctcacctcac gacggtaggt gatgcttcgt tcccgttcca gatgagcgcg
      601 ggttttagcg gcttcgaccg gacgctcacg ttccggaagt tcaatcgggc gttgcgggcg
      661 cgggtggcgg tttaccggaa cgactttgca cgggcgctca ccatactggt gccggactcg
      721 acgttcatcg acgatggctc gacactcacg acaggggtgt accacacctt taccaccgcg
      781 ccaggcgatt cgaccaacaa cctgttcgac cccaacgccc gggcgttgct ggcgcacccc
      841 tcgctcgaga ccgatgcgca gctacaggct ggcggggcac ctgaccagcg attcctcaac
      901 aagaccacac tgctgtccag cgcccatacc agctccgggc tcagctcgaa ccgggcgctg
      961 aacgtctacg cggggccgtc cgccccggtg ccgattatca ggaacgagga actcattctg
     1021 cttcgtgccg aagcgaacat cgggatcaac aacctggctg gcgcaattcc ggacatcaac
     1081 accgctcgcg cagccggggg gttgacgccg ctggtcgcgt ttgcgagcca cgatgaggcg
     1141 ctggcggagt tgctgtacaa caagcggtac tcgctcctct tcgagggtgg gcaccgctgg
     1201 atcgacatgc ggcattacgg gttgctcacg agcgatccac atcctccctt caccacattg
     1261 cagaaggagg tcacgccctc cggcaccaga cacttcttca atcggttccc gttcccgacc
     1321 aacgagtgtc tggcgcggac aacgcagccc gcgaggggct gcacgctgga accgggcagc
     1381 tgagccggag cagcgccacc gctgaaaaat gcgaaggggc ccgtggttcg ggcccctttc
     1441 ctttccactt tgtcggaacg gctatttctc ggtcgtcccg gtaagaatcc cctgtctgat
     1501 tcgcactgcc ttcggcgaag gattcgggtc cagctcaacg tgggcgttca cctgctcgag
     1561 gagacgaacc ttgccggtga gcgtcagcgt gtccgctccg cggcgcacct tgatcgggaa
     1621 gtcctggccc tcggcgtcct tgaaacgggc gcgataggcc ggcccgaaat tgggatcggt
     1681 aatggcgaca tcgcccagag cgatcagaat gtcgccggca tgcacaccgg cagtttctgc
     1741 ggctccgcct ggctgaatcg ccgtcacctt tacgccggcg gaatcaggag ccccggcgac
     1801 tcccagccgc ggctcccgga tggtgtccga tgcaagccgc atccctgcca gcttcagcat
     1861 tgcgtcgtag gggtacgggt ctcgtccgtc gatgtatctc gcatcgaagt cggcgaatga
     1921 tttcccggcg ctggcccggc tgaccgcgcc ccaccagtcg gcggcggtga atccccgacc
     1981 gctcttgtag gtggtccgat agagctcccg catcacatcg tcgagcgatc gacggttgtc
     2041 gctcgcgtcc cgaatgacaa tgtcgagcag cagccctgcc agcgaaccct tgggatagta
     2101 caggtagccc gagccgtcgg tcgggtggat ccaggtagac agcgacgcat cctgcagggc
     2161 tgtgggtgcg gtctccgcga cggtctgaat cttctccgcg gtaagtccca tgaagatggt
     2221 cgagtccacg atcttcccgc ggacgagtgc caggtcggcg taatagtccg tgatgccctc
     2281 gcttacccac aaccagggcg ttggctcgac tcggtcgtag ctgtagggga ccatgtcggc
     2341 cgggcgaagg cgcttcacgt tccaggcgtg gaagatctcg tgggccgtga tcgacgcgag
     2401 cagcggggtg ccaagcatca tcggtgcgta cacgccgaca tgggagctct gatgctcgag
     2461 ggcgctgcct ccttgatacg acgagtcgaa gatcgtcatc acggtgtagt tattccaggg
     2521 tacctcctga aacaccgcag ccatggctgg aatcgccttg gcgatctgat cccacagcgc
     2581 cgctcgggtc tcccccttga ggatgccgcg agggtagctc gccagccggg tggtcttccc
     2641 gtcgaccacc atactgtcaa agtcgaatcg gccgacgaag aacggcatgt cgaccaggtc
     2701 gtggtagttc ccctcactcc aggtgaatgc cgcaccggct gacttcattc cggtcgcgac
     2761 cagccaatcc ggctgcgtct ttaccgtcac ggtggcgggg aagtcgaagc cgcggccttc
     2821 ggggtagggg aagatgttgg tgccgttgaa gagcagaaag tcggggcggg accacgccat
     2881 cgcattgtcc agcgtgtccg ccagatagtc gaatcgcacc ataacccgtc ccggcccagt
     2941 ggtgcgaatc ctccaggtat caggatccgc cttgttccac gccagagggc gatcgtcgct
     3001 gctgctcgct gagaagcgtg aaatccagcg agcaaagctg ctgatctcat acgcgcccgg
     3061 cgtccacgcc gggaccgaca ggagcaccgg atccttgccc gccgcgacat cgaaagacat
     3121 tacgacccga atcgtgcgcc gcaccgcggt tgccgagtcg aaggtcacgt cgtagcggat
     3181 gttgctgatc ggcgcggagc gcggcacggt cggcgtggaa cgagcagggg cggtggtctt
     3241 cggcttcccg gcctgctgcg cggtcgacgc actggcgccg gccaagagag cgataccagc
     3301 gaggagcggg gtgcgcgagg cgatcatgga atgtccgggt gaggaatgag ccgctagacc
     3361 gatggcggtg tcgatagcct gctgagttcc ggcgcagggc ccgtagcccg ggcctcgatc
     3421 ctggcagcct caggggccga ggcgagctcg ccctcccacc ggcccacgac ggcggtggcc
     3481 aggcagttgc cgaccagatt gacagccgtg cgcgccatat ccatgacctc gtccacaccg
     3541 agaataacgg cgataccctc gagcggcaga ccgaacttgg tcagagtgcc ggccagcacg
     3601 acgagcgatg cgcgcggaac ggcggcgatg cctttgctcg tgatcagcag cgtcagcatc
     3661 atgaagagct gctgacccaa actcaggtgg acgcccgcgg cctgggcgac gaacagggag
     3721 gcaacgccaa gatacagagt cgagccatcc aggttgaacg agtagcccgt cggcatgacg
     3781 aacgccacga tccgcttcgg aacgccgaac tccaccatcc gctgcattgc aagcggcagc
     3841 gcggcatcgg acgaagtggt ggtaaacgct atgagcgcgg gatccttcac tgttcgggcg
     3901 aatcggagga tgtcgatgcg ggcgatcaaa gccgcgggga gaagcgccac gaaggcgaac
     3961 acgaacagcg cgccgtacag tgtcagaacc aacttgccga gattgatcag cacgcccaat
     4021 ccgccatggc tcaccgcgac agccattgcc gccccgacgc cgatcggggc gaacttcatc
     4081 acgatcgcca cgaatttgaa catgacttcc atcagccctt cggcgaagcc gagcatggcc
     4141 tccttgggcc gtcccttcac ttgggtcagc gcgatcgcga acagcgtcgc ccagaaaacg
     4201 acctgaagca cttcgccgtt ggccgcagct tcgaccacgc tccgcggcac gacatgctcg
     4261 aggaactcgg cgaacgtggt gggagcgggt tgagggagcg ctgcgtgggg atcaggtggg
     4321 gggagtcgga cgcccacgcc cggcttgacc agattccccg ccagcagacc gatcgcgagg
     4381 gcgatggtgg tcatgaccca gaagtagccg atcgacttga tcgccagccg cccgattcgc
     4441 ttgagatcgt ctccgtgccc cgcgatgccg atcaccagcg tcccgaagat gatcggcacg
     4501 acaaccgact tgatcattcg gaggaacgtg ctggagagag gcttgaggcc gagggcgaat
     4561 tcctgggggg cgagccagcc gatcagaatg ccggcggcca tgccgacgaa gatccagccg
     4621 gtaagcgaga tgcgtcgaac ggctgtcagc atgggaagaa aagcgtgaac ggtgaacagt
     4681 aaacggcaaa cgggaggttc ccgtccccgg agcaaccccg tccactgttc actgtttacc
     4741 gttcacctgg ccggcgctgc gccggccaaa gcgctggctt tgtgttcgag ggtagccaga
     4801 gccgcgccat acgatgccgc gaacttggtc acaccctcat cctccagctc cctggtcagc
     4861 gatgggaaat cgaggccgag tttggccagt gacgccatct ggctgtccac ggcggcgata
     4921 ccggtttgga ttcggacttc cggcttgcca tggtcacggt aggcggcgaa ggtctccggc
     4981 ggcagcgtgt tcacagtatt gggagcaatc agcgcctcga catagtgcac ctcggagtac
     5041 cgtggatcct tggtgctcgt ggatgcccag agtggccgtt ggggccgagc gccccgctcg
     5101 gcgagcgcgg accagcgaga ctcggcacgg cgcttctcga aggctcgata ggccaggcac
     5161 gcgtttgcga ttgcgatctt gccccgcaac cgactggggt cgccgatgcg atcgagcatc
     5221 gggtccagct tggtgtccac ccgactcacg aagaagctgg caaccgatgc gatccaatcc
     5281 actggcaagc cgcgcgccaa tcgggcctcg agcccggcct ggtaggcggc gatgaccccc
     5341 tcatatcgct cgaccgaaaa cagcaacgtg atgttgacat tgatgccctc ggcgatgcac
     5401 tgcgtcaccg cggggaggcc ttccaccgtg cccggaatct tgatcatcgc attgggccgt
     5461 ccgacggtcc gccagagacg ttcggcctcc acgacggtcg cggccgtgtc gcgcgcgagg
     5521 gcgggcgaca cctcgatgga aaccagtcca tcctgtccac cggctcgctg atataccggc
     5581 aggaatgcat cgcacgcctc gcgcacatcg gccacggcga gcgcctcgaa cacctccatc
     5641 gagctgcgcc ctctggaggt cagctgtctg atgtcctcgt cgtagaggtc gctgccggcg
     5701 acggccttct cgaagattgt gggattggtc gtcatgccga gcaggccgtc atcggcgatc
     5761 aaacgggtca actgacccga cgcgaggaga tcgcgggtga tgaagtcgta ccaggggctc
     5821 tggccaagct cacccagccg aaccagggga ttggttgtca tgcggttctc ccgagcaccc
     5881 ggcggacggc tgccgcggcg tggtccggag tgaaatggaa ctcggaaaat aggcgttccg
     5941 ccggagcgga ggcgccgaag tgatcgagag cgagcatctc gccggcgtcg gtggtgtaac
     6001 gaagccagcc gaaaggcgat gccgcctcga tgccgactcg ggcccgaatc gtgggcggaa
     6061 gcacggaatc ccgatactcg cggggctgcg cctcgaacag ctcccacgat gggagcgaga
     6121 ccacccggcc gcgaatgccc tcagccatca attgctgagc tgccgccagc gcaacgtgca
     6181 cctcggatcc agtggccatg aggatcgctt ccggcccgcc cggcgagtcc cacaacacgt
     6241 agccaccgtg ctggacaccc gctgcgggcg ctaaggtcgc tcgatccagc acagggagtt
     6301 tctgtcgagt gaggaccagc gccaccggcc cgctgcgatg cgcaatggcg actcgccagg
     6361 cttcgaccgt ctctgtcgcg tcggcgggac ggatcaccgt catccccgga atcgaccgca
     6421 gcattgcgag gtgctcgatg ggctgatggg tcggcccgtc ttcgcccacg ccgatcgagt
     6481 catgggttcc cacgtagatg accgggagcc gcatgatcgc agccaggcga atcgacggct
     6541 tcatgtagtc ggtgaagacc aggaacgtgc tgccgaacgg ccgaagtcca ccgtgggccg
     6601 caatgccgtt gagtgcggct cccatcacgt gctcgcggac accccagtgg atctgacgac
     6661 cggtgctact cggtccga
//
LOCUS       DASRJQ010000533         4610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3304048, whole genome shotgun sequence.
ACCESSION   DASRJQ010000533 DASRJQ010000000
VERSION     DASRJQ010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3304048"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VFB89_12200"
     CDS             complement(<1..763)
                     /locus_tag="VFB89_12200"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HZM28115.1"
                     /translation="MSQNQWAQSYVDSSRLSTLSVGLRAGRQQRPRLWILVLLSLACD
                     QSAAADRPTLIDRLLQLELDRGSLCSELQDSQAPRAQLSAAAARLREGLGTHERGTGS
                     SDEAQQVVQALNEVVFDHLGIRPSQDLHDPCNLFLSSVLARKQGYCVGIAAVYLVLAE
                     QLDLPVYAVATPSHVFLRYDDGKTRINIETFSRGAPTPNEQYIAEQKIAPRTLERGIF
                     LRALTADEFLAQVHNNLGVVYSHQQNYAAATREYDT"
     gene            1035..2735
                     /locus_tag="VFB89_12205"
     CDS             1035..2735
                     /locus_tag="VFB89_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28116.1"
                     /translation="MSMTLGRAVRTIPIAIIVLAFLFGGAGDVLAVDENETVGFQSNH
                     LFESGVFGENIDVLNGGLNLSIPIGSRYQVSQYLSYQLQLAYSSKVWDHTTYSGSNPV
                     DKLMGRSSMGLGFRLHMGRIYKDVELVGPGKRQCTWYLVTPDGNQHEIPVSPVAGSDD
                     ACTQALPAPNVQTIDTTYFKLNGFDALIGWTGPPETAPILTAKTSDGRLEYTFGKFIQ
                     VANLNRQDSSTPGDGGYTTNHNRDFGGWYVTRIDDLKSGGSAWVEVEYETEAGYQHAI
                     SRIEDSLGREITFTNECEPATPPASGCQQVSVGPSDPNRVAVHTTEISVPAFKDSAAI
                     DNDISATYTFSYGWGTVDHVDYAALGCSPPPATCPQSSVNYLTAIHYPDFDNQIPKPG
                     PGSAVAYSMAFGYSRYGEIITRRIPTGAIFDYAWDVYYYNTGDTRQIVGKTLYMSEED
                     RNPPEGEPQEAGVWTYQRQTFDGGTALTNPLYVNVTDAAGNETVYHYRASLFDQTPPP
                     NPPPDPPPPTDYDDGYTPEWDDGVNVRIDYYSGTGATRTLILSTLPKFVLRSSDKSVE
                     "
     gene            2782..4209
                     /locus_tag="VFB89_12210"
     CDS             2782..4209
                     /locus_tag="VFB89_12210"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HZM28117.1"
                     /translation="MWERDDRESVEAEVRPEDQQTPGGAPARPGLFSELGTGITTGEV
                     VVPASSEAEERAEDEAPVDQRAPSPPVLTGRRKGGPMRRVLEAEPEPHVEGVALSGEQ
                     RLLILDAWTRSGLTAGEFGRIAGVSSHTLYAWRRRFDEFGPAGLDERRRGAPTGSRVP
                     EATKRAILMMKGQHEEWGCERIHDMLMRSEGFMASAGAVARVLREAGYESEEQPTERH
                     PDKPREFERARPNQLWQSDLFTFLLKREGRRLYLIGFLDDHSRYMVGYGLYASSSGAL
                     VREVFEQAIANYGPPEEVLTDQGPQYHTWRGTSEFARLCARRGVRQLVARARHPQTLG
                     KIERFWGTLWRECVEQAVFQGLEDARRRVGLFIDHYNFQRTHQGLGGLVPADRYFSAA
                     PQVLETLKKRVEANALDVARHGTPRQNVYLTGRVGEAGITMHGEGGKLVVQTTDGGRQ
                     EVDLTAPGRRAPVTDVVPTDEGGEA"
     gene            4206..>4610
                     /locus_tag="VFB89_12215"
     CDS             4206..>4610
                     /locus_tag="VFB89_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28118.1"
                     /translation="MSAATKTTRPGPQPISGDEAAKRSAAVILEVLAGLRTPTDGSQA
                     LGITSMRYYVLERRALEGMVQALAPRPKGKRRRPEDALAEVRREKVHLEREVGRLQAL
                     VRAAQRTMKLPPPPSRERKKGEARRPRRPQVRA"
BASE COUNT          891 a         1270 c         1687 g          762 t
ORIGIN      
        1 ctgtgtcgta ctctcgggtc gcagctgcgt agttctgctg atgcgagtag acgacgccga
       61 ggttgttgtg gacctgggcg aggaactcat cggcagtcag ggcgcgcaga aatatccctc
      121 tctcgagagt ccggggcgca attttctgct cggcgatgta ctgctcgtta ggagtcggag
      181 caccccggct gaaggtctca atgttgattc gggtcttgcc gtcgtcatac cggaggaaca
      241 cgtgcgacgg tgtggcaact gcgtagaccg ggaggtccag ctgctcggcc aggacgagat
      301 agacagctgc gatgccgacg caataaccct gcttgcgggc cagaacgctt gataggaaca
      361 ggttacaggg gtcgtgaagg tcctgggagg ggcggattcc caagtggtcg aagacgacct
      421 cgttgagggc ttgtacgact tgctgtgcct cgtcgcttga gcccgttccg cgctcgtgcg
      481 ttcctaaccc ttctcgcagg cgggcagctg ctgccgagag ctgagcccga ggcgcctgac
      541 tgtcttggag ctccgagcac agggagcctc tgtcgagctc tagctgcagg agtcgatcga
      601 tcagggttgg ccggtccgct gcggcggatt ggtcacaagc cagagacaga aggacgagaa
      661 tccacaacct tggccgctgt tgccttcccg cgcgcaggcc gacactaagt gttgacagac
      721 gggatgaatc gacgtaagac tgcgcccatt ggttttggct cacgctcagc ttcggggaag
      781 ccaggatgat tcaccccggg gagacggtgt ccgtcgccag gatagcactg gtgtgcgtgg
      841 ggagcagggt tgtccgtgag aactggacct tcggccaggt cacgccgtca gaagggctga
      901 actagaaaca cagtggcgcg gtgcttgctc agaaggccaa ggctatcgtg accccttcgc
      961 caggggtctg tctcgcttga ggaagccgca gtcaacatgc aggagtcttc gccctcgctg
     1021 gagggcccag agggatgtcc atgacactcg gccgcgccgt ccgcaccatc ccgatcgcga
     1081 tcatcgttct tgcctttctc ttcggcggtg caggagacgt tctagccgtt gatgagaacg
     1141 agacggtcgg ctttcagagc aaccatctct tcgagagtgg cgtgttcggc gagaacatcg
     1201 atgtcctgaa tggggggctc aacctttcga tccctattgg ttccaggtat caggtgagcc
     1261 agtatctcag ctaccagctc cagctagcct acagttcgaa ggtttgggac cacacaacat
     1321 attcgggtag caacccagtc gataagctca tgggccgcag cagcatgggg ctcggattcc
     1381 gcttgcacat ggggcggatt tacaaggatg tagagctggt cggcccaggg aaacgccagt
     1441 gcacgtggta tctggtcaca cccgatggga accaacacga aataccggtg tcaccggtcg
     1501 caggtagtga tgacgcgtgc acgcaagccc tcccagcacc caacgtgcag acaatcgaca
     1561 cgacctattt caagctgaat gggttcgacg cactcattgg atggacaggc ccgccggaga
     1621 ccgcgccgat tctaacggcg aagacttcag atgggcgatt ggaatatacg tttggaaagt
     1681 tcattcaagt cgcgaacctg aatcggcagg actcatcaac gccaggagat ggggggtaca
     1741 ccaccaacca caaccgggac ttcgggggct ggtacgtcac acggatcgac gatctgaagt
     1801 cgggaggcag cgcatgggtt gaggtggagt acgagactga ggccggatat cagcacgcga
     1861 tctcgcgcat cgaggacagc ctgggccgag agatcacttt cactaacgag tgtgagccgg
     1921 caacacctcc agcgagcggt tgccagcagg tttcagtcgg cccgagtgac cctaaccgcg
     1981 tcgcggttca tacaacggaa atatcggtgc ccgccttcaa agattcggcc gcaattgata
     2041 acgacatttc cgcaacctac acgttcagct acggatgggg aactgtcgac cacgttgatt
     2101 atgctgcgct tggttgcagc cctccacccg ctacttgtcc gcagagctcg gtgaactatc
     2161 tgacggcgat ccactatccc gacttcgaca accagatacc aaaaccgggc cccgggtctg
     2221 cggtcgccta tagcatggcg tttggatact cgagatacgg cgaaattata acgcggagga
     2281 ttccgaccgg agcgatcttc gactatgcgt gggacgttta ctactacaac accggggaca
     2341 cgcgccagat tgttggaaag acgctgtata tgtcggaaga agacaggaac cctcctgagg
     2401 gagaacccca ggaggctgga gtgtggacgt accagcgcca gacgttcgac ggggggacgg
     2461 cgctcacgaa cccattgtac gtgaacgtca cggacgccgc cggcaatgag accgtctatc
     2521 actatcgggc ctcactcttt gaccaaaccc ctcctccaaa tcctcccccc gacccaccgc
     2581 cgccgaccga ctatgatgac ggctacaccc cggagtggga tgacggggtg aatgtccgca
     2641 tcgattacta ctcgggaaca ggcgctacga gaaccctgat cctgtccacc ctcccgaaat
     2701 tcgtcctcag atcaagcgat aaatcagtgg agtaaaggcc tgcttccctg agcggccgga
     2761 ggccgcgaga gggagggcag catgtgggag cgtgacgatc gagagtccgt agaggcggag
     2821 gtgcgtccgg aggatcagca gacgccgggc ggtgcgccgg cgaggccggg gctgttcagc
     2881 gagttgggga cggggatcac gacaggggag gtggtggtgc cggcgtcgag cgaggcggag
     2941 gagcgggccg aggacgaggc gccggtggac cagagggcgc catcgccgcc ggtgctgacg
     3001 gggcggcgga agggcgggcc gatgaggcgg gtgctggagg cggagccgga acctcacgtt
     3061 gaaggcgtgg cgttgagcgg cgagcagcgg ctgttgatcc tggacgcatg gacgcggtcg
     3121 gggttgacgg cgggggagtt cgggcgtatc gcgggggtgt cgtcgcacac gctgtacgct
     3181 tggcggcgtc ggttcgacga gttcgggccc gcggggctgg acgagcggcg gcggggggcg
     3241 ccgacgggaa gccgggtgcc ggaggcgacg aagcgggcga tcctgatgat gaagggccag
     3301 cacgaggagt gggggtgcga gcggatccac gacatgctga tgcgctcgga ggggttcatg
     3361 gcgagcgcgg gggcggtggc gcgagtcctg cgggaagcgg ggtacgagag cgaggagcag
     3421 ccgaccgaac ggcatccgga caagccgcgg gagtttgagc gggcgcggcc gaaccagctg
     3481 tggcagtcgg acctcttcac gttcctgttg aagcgggagg gacggcggct gtacctgatc
     3541 gggttcctgg acgaccacag ccggtacatg gtggggtacg gactgtacgc gtcgtcgtcg
     3601 ggggcgctgg tgcgggaggt tttcgaacag gcgatcgcga actacggtcc gccggaggag
     3661 gtgctgacgg accaggggcc gcagtatcac acctggcgtg gcacgagcga gtttgcgcgg
     3721 ctgtgcgcgc ggcgcggggt caggcaactg gtggcgcggg cgcgacaccc gcagacgctg
     3781 gggaagatcg agcggttctg gggaacgctg tggcgggagt gcgtggagca ggcggtgttc
     3841 caggggctcg aggacgcgcg ccggcgagtc gggctgttca tcgatcacta caacttccag
     3901 cgcacgcacc aggggctggg agggctggtg ccggcggacc ggtacttctc ggcggcaccg
     3961 caggtcctgg agacgctcaa gaagcgggtg gaggcgaatg cgctggacgt ggcgcggcac
     4021 gggacgccgc ggcagaacgt gtacctcacg gggcgggtgg gcgaggcggg aatcacgatg
     4081 cacggcgagg gtggcaagct ggtggtgcag acgacggacg ggggacggca ggaggtggac
     4141 ctgacggcgc cgggccggcg ggcgccggtg actgacgtcg tcccgacgga cgagggaggt
     4201 gaggcatgag tgccgcgacg aagacgacgc gaccgggacc gcagccgatc tcgggggatg
     4261 aagcggcgaa gcggagcgcg gcggtgatcc tcgaggtgct ggcagggctg cggacgccga
     4321 cggacggatc ccaggccctg gggatcacgt cgatgcggta ttacgtgctg gagcggcggg
     4381 cgctggaggg gatggtgcag gcgctggcgc cgcgaccgaa ggggaagcgg cggcgtccgg
     4441 aggatgcgct ggcggaggtg aggcgggaga aggtgcacct ggagcgcgag gtgggtcggc
     4501 tgcaggcgct ggtgcgggcg gcgcagagga cgatgaagct gcctccaccc ccatcgcggg
     4561 agaggaagaa gggcgaggcg cgccgtccgc gccgacccca ggtgagggcc
//
LOCUS       DASRJQ010000534         2320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_487613, whole genome shotgun sequence.
ACCESSION   DASRJQ010000534 DASRJQ010000000
VERSION     DASRJQ010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487613"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VFB89_12220"
     CDS             complement(<1..462)
                     /locus_tag="VFB89_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28119.1"
                     /translation="MSPAHDPEDKAAADPDAPPPAVRRSLRYRVVHSVITVFAAIGGA
                     VVGFMVTLALVVLEARFESYIFAMEDLMAFRWDLIPIPLGAWAGYRLHHRRSHGVGWA
                     TLCGLAGLVVGGVAGTVVGSIVWSDSAAPWAGAVIGGAVDWWRVASRPSGFV"
     gene            545..1045
                     /locus_tag="VFB89_12225"
     CDS             545..1045
                     /locus_tag="VFB89_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZM28120.1"
                     /translation="MSVQHPIEVTVNGTRHAREVEPRLLLSDFLRHDLALTGTHVGCE
                     HGVCGACTVLLDGEAVRSCLVFAVQAHGHSVETVESLATGETLHPIQQAFHETHALQC
                     GFCTPGILMTLIPFLQDHPAPAESDIREILSGHLCRCTGYANIIAAVQRAVRDGSAAS
                     AAEGPR"
     gene            1072..>2320
                     /locus_tag="VFB89_12230"
     CDS             1072..>2320
                     /locus_tag="VFB89_12230"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HZM28121.1"
                     /translation="MLRLEDARLLTGRALFVDDVELPGMLHVAFLRSDDAHGRIRSID
                     ATAARARRGVLAVYTASDLGDYWRPGPLLVPPPPVPGLIFNAATQVPLARDKIRHCGE
                     PLAMVVAESRYLAEDALEDIVVDVEPLPAVADLEAALLPDAPLVHEQFGSNLAAQVHQ
                     RKGDYASAKAAAALVISRRFLYDRGASAAIENRGVVASWDARAGELTVWDTTQAPIPI
                     RNGLAAMLGLGESHVRVIAPFIGGGFGPKIMMFYPEEVLIPWAAMRLGRPVKWIEDRR
                     ENFYATTQERGQIHDAELAVSRDGKILGVRDAFLHDTGAYDPYGLTVPINSQCTLLGP
                     YVVPHYDSQFQAVFTNRPIVTPVRGAGRQHGVFVMERLLDLAARDLGIDRVEIRRRNY
                     IQPDAFPYDHEILFQDFRPLVYDS"
BASE COUNT          371 a          816 c          758 g          375 t
ORIGIN      
        1 gacgaatccg gagggacgcg acgcaacccg ccaccagtcc accgctcctc cgatgaccgc
       61 cccggcccag ggcgcggcac tgtcgctcca gacgatcgag ccgacgaccg tgccggctac
      121 gccacccacc acgagccccg cgaggccgca gagagtggcc caccccacgc cgtgggatct
      181 tcgatgatgg agtcggtatc cggcccaggc gccgagaggg atcggaatca gatcccagcg
      241 aaacgccatc aggtcttcca tggcgaagat gtacgattcg aatcgggcct ccagaacgac
      301 cagcgcgagg gtgaccatga agcccaccac ggcaccccca atggccgcga agaccgtgat
      361 gacggaatgc acgacgcggt agcggaggct gcgccggact gccggcggag gtgcgtcggg
      421 atcggcagcc gccttgtcct caggatcgtg tgccggactc atgtcgttcc tttccgcgaa
      481 tacccttgcg cgcccccacc ccgggcgtat cgttcaaccc ttccctcccg gagcgagcgc
      541 gcgcgtgagc gtccagcacc caatcgaggt gacggtcaac gggacccgcc acgcacgcga
      601 ggtcgagccg cggctgctgc tcagcgactt cctgcggcac gatctcgcgc tgaccggaac
      661 ccatgtgggg tgcgagcacg gtgtctgcgg cgcctgtacc gtcctgctcg atggcgaggc
      721 ggtccgctcc tgcctcgtgt tcgccgtcca ggctcacggg cactcggtgg agacggtgga
      781 aagcctcgcc accggcgaga ccctgcaccc gattcagcag gcctttcatg aaacccacgc
      841 cctgcagtgc ggattctgca ccccgggcat cctcatgacg ctgattccct tcctgcagga
      901 tcatcccgcg cccgccgaat ccgatattcg cgaaattctt tcggggcatc tctgccgctg
      961 caccggatac gccaatatca tcgccgccgt tcagcgcgcc gttcgcgacg gatcggctgc
     1021 gtccgccgcg gagggtcccc ggtgaatttc gaaagctaca tggggcggcg cgtgctccgg
     1081 ctggaagacg cgaggctcct caccggccgg gcgctcttcg tggacgatgt ggagctgccc
     1141 gggatgctcc acgtcgcctt cctgcgcagt gacgatgccc atggtcgtat ccggagcatc
     1201 gatgcgaccg ccgcgcgcgc gcgccgcggc gtgctcgcgg tctacaccgc ctccgatttg
     1261 ggcgactact ggcggcccgg ccctctgctg gtgccgcccc cgccggtgcc ggggctgatc
     1321 ttcaatgccg cgacccaggt cccgctcgcg cgcgacaaga tccggcactg tggcgaaccg
     1381 ctggcgatgg tggtggccga gagccgctac ctcgccgagg atgccctgga agacatcgtc
     1441 gtggatgtgg agccgctccc ggcggtcgcg gatctcgagg cggcgctgct gccggacgct
     1501 ccgctggtgc atgagcagtt cggcagcaac ctcgccgccc aggtgcacca gcgcaaaggc
     1561 gactacgcct cggcgaaggc ggctgcggcc ctggtcattt cccgccggtt cctctacgac
     1621 cgcggcgcct cggcggcgat cgagaaccgg ggggtcgtgg cctcctggga tgcgcgcgcc
     1681 ggcgagctga ccgtgtggga cacgacccag gcgccgatcc cgatccgcaa tgggctcgcg
     1741 gccatgctcg ggctgggcga atcgcacgtg cgcgtgatcg cgccgttcat cggcgggggc
     1801 ttcggtccca agatcatgat gttctacccg gaagaagtgc tgattccctg ggccgccatg
     1861 cgactgggac gtccggtcaa gtggatcgag gaccggcggg agaatttcta cgccacgaca
     1921 caggagcggg gccagattca cgatgccgag ctggcggtat cgcgtgacgg gaagattctc
     1981 ggggtgcgcg acgcgttcct gcacgacacc ggcgcctacg atccctacgg gctcaccgtt
     2041 ccgatcaaca gccagtgcac gctgctcgga ccctacgtcg tgccgcacta cgacagccag
     2101 ttccaagcgg tcttcaccaa ccggccgatc gtgacgccgg ttcgcggcgc cggccggcag
     2161 cacggcgtat tcgtaatgga gcggctgctc gacctcgccg cccgcgacct cggcatcgat
     2221 cgggtggaga tccgccggcg caattacatc cagccggatg cctttcccta tgatcatgag
     2281 atcctgttcc aggatttccg gccgctggta tacgacagcg
//
LOCUS       DASRJQ010000535         2418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_201378, whole genome shotgun sequence.
ACCESSION   DASRJQ010000535 DASRJQ010000000
VERSION     DASRJQ010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201378"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1746)
                     /locus_tag="VFB89_12235"
     CDS             complement(46..1746)
                     /locus_tag="VFB89_12235"
                     /inference="COORDINATES: protein motif:HMM:NF028259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28122.1"
                     /translation="MVWRQYRGPRLWIVLSPKWQSARARLLQERDGAGPRLFLLTVLA
                     LAFWSGVFGIVYRVLRYFRTVPEIGDLLSTKLLGLVLLAFSSILLLSNVITALSSFFL
                     AKDLDLLAASPVHSIRFYLAKLTETLLHSSWMVALLGVPLFAAYGIVYHGGPLWPVAV
                     LAAFIPFLLLPAVVGTALTLLLVNIVPARRAKELLSLIGIGAIASLVLVLRVAQPEQL
                     VRPEGFRSLADFISMLQAPTNPWLPSEWAMSALMNWLLRVGDPQPLVFLWGTSAISLA
                     VGSVLFRRLYRRAFSKAQESAPVPAGSRWTAALEMLLQWLPADRREFILKDTRLFFRD
                     PTQWSQLILLAVLIVVYLFNIQALPLFSGERFPASVLTLVVFLNLGLAGFVLAAVAAR
                     FLFPSVSLEGRQMWLLRSSPLDLRAMLWSKYWIGTVPLLILALLMTTVTNLILKASPF
                     MSAVSLVTMVVFTLAASALALSFGAFYPRFDTENAAQIPTSFGGLVYMMASVTLLALV
                     IMLEAPTVTAQIRAEQYGVRVAWNPWMAVPLASAGIVCVTATLLSLRLALSRVQRLEV
                     "
     gene            complement(1749..>2418)
                     /locus_tag="VFB89_12240"
     CDS             complement(1749..>2418)
                     /locus_tag="VFB89_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZM28123.1"
                     /translation="GFLGPNGAGKTTTFRMIAGIIVPTAGRIEIGGVDLVRQPLVAKR
                     RLGYIPDRPFVYDKITGAEFLRFVAALYNAEDASLERRIDELLDLFELMPWKHELTES
                     YSHGMRQKLIIAGAFIHRPEVIVVDEPMVGLDPKGARLLKDLFRQYVNRGGTILMSTH
                     TLEVAESMCDRIAIMQSGRILAQGTMDELRVQTASGNASLEDLFLRLTGGFREHQLDT
                     ILEV"
BASE COUNT          521 a          765 c          771 g          361 t
ORIGIN      
        1 gtccgacctg cggcggaaag atgcgcgacg tgtttcaggg tgggttcaca cctccaggcg
       61 ttgcactctg ctcagggcca gtcgcaggga cagcagtgtc gcagtcacac acacgatccc
      121 ggcgcttgcg agcggcaccg ccatccaggg attccaggcc actcggaccc cgtactgttc
      181 ggccctgatc tgagccgtca ccgtcggagc ttccaacatg attaccagag cgagcaaggt
      241 cacgctcgcc atcatgtaca ccagacctcc gaagctggtc ggaatctgag ccgcgttctc
      301 ggtatcgaat cgcgggtaga acgcgccgaa gctcaacgcc agcgcgctcg ctgccaacgt
      361 gaagacgacc atcgttacca ggctcacggc ggacatgaac ggcgaggcct tgagaatgag
      421 gttggttacg gtcgtcatga ggagcgcgag gatgagcagc gggacggtcc cgatccagta
      481 cttgctccag agcatcgccc ggagatcgag cgggctcgac cggaggagcc acatctgccg
      541 cccttcgaga gacacggacg ggaacaggaa gcgggccgcg actgccgcaa gcacgaaccc
      601 tgccaatccc aggttgagaa agacaaccag tgtcagcacc gaagcaggaa agcgctcgcc
      661 cgaaaacaga ggcagggcct gaatgttgaa cagatagacc acgatcaaga ccgccagcag
      721 aatcaactgg ctccactgcg tcgggtcccg gaagaagagt ctcgtatcct tgaggatgaa
      781 ctcccgccgg tcggccggga gccactggag cagcatctcg agagccgcgg tccagcggct
      841 gcccgccggg acgggggcgc tctcctgtgc cttcgagaac gctcgccggt agagccttcg
      901 aaacaaaacc gatcccacgg cgaggctgat cgcactggtg ccccagagaa agaccaatgg
      961 ctgaggatcc cccacccgaa gcagccagtt catcagcgcg ctcattgccc actcgctggg
     1021 cagccaggga ttggtcggcg cctgcagcat ggagatgaag tccgccaacg agcggaatcc
     1081 ctccggccgc accagctgct ccggttgagc cacccggagg accaggacca ggctcgcgat
     1141 cgcaccgatg ccgatgaggc tcagcagctc cttggcccgc cgcgcgggga caatgttgac
     1201 cagcagcagg gtgagggccg ttccgacgac ggcgggaagc agcaagaagg gaatgaaggc
     1261 agcgaggacg gcgaccggcc aaagagggcc accgtggtag acgattccgt acgccgcgaa
     1321 gagcggcacg ccgagcaacg ccaccatcca ggatgagtgc agcagcgtct ccgtcagctt
     1381 ggccagatag aaccggatgg agtgaacggg agatgcggcc aacaggtcga gatccttggc
     1441 gagaaagaag ctcgagagcg ccgtgatcac attggacagc agcagaatcg acgagaacgc
     1501 gagcagcacc agcccgagca gcttggtcga gagcagatcg ccgatctccg gtaccgtccg
     1561 gaagtaccgg agcacacggt atacgattcc gaagacccct gaccagaagg cgagcgcaag
     1621 tactgtaagc agaaagagac gcgggcccgc accgtcccgc tcttgcagca accgcgctcg
     1681 cgcgctctgc catttcggac tgagcacgat ccacagccgg ggaccgcggt actgccgcca
     1741 gaccacgatc agacctcgag gatggtatcg agctggtgct cgcgaaaacc gccggtaaga
     1801 cggaggaaca gatcctcgag actggcgttg cccgatgccg tctgcacccg cagctcgtcc
     1861 atggtgccct gggccagaat gcggccgctt tgcatgatgg ctattcggtc gcacatgctc
     1921 tcggcgacct cgagcgtatg cgtgctcatc aggatggtcc cgccccgatt cacgtactga
     1981 cggaagagat ccttgagcag ccgggccccc ttgggatcga gtcccaccat gggctcgtcc
     2041 accacgatca cctcgggccg atggataaac gcgccggcga tgatgagctt ctgtcgcatc
     2101 ccgtggctgt atgactcggt gagctcatgc ttccagggca tcagctcgaa gaggtccagc
     2161 agttcgtcga tccgccgctc caacgaggca tcctcggcgt tatacagggc ggccacgaag
     2221 cggaggaatt ccgctccagt gatcttgtcg tagacgaagg ggcgatcggg gatgtagccc
     2281 agccgccgct tcgcgaccag cggctgtcgc acgaggtcca caccgccgat ctcgatccgc
     2341 ccggcggtgg gcacgatgat cccggcaatc atgcggaagg tggttgtctt gccggcgccg
     2401 ttgggcccca ggaagccg
//
LOCUS       DASRJQ010000536         3383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3196626, whole genome shotgun sequence.
ACCESSION   DASRJQ010000536 DASRJQ010000000
VERSION     DASRJQ010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3196626"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VFB89_12245"
     CDS             <1..643
                     /locus_tag="VFB89_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HZM28124.1"
                     /translation="LVAGNVATEAGARELVRRGADAVKVGIGPGSICTTRVVTGVGVP
                     QVTAIMDAVRGAGDVPVIADGGIKYSGDVVKAIAAGASSAMMGSMLAGTEESPGEAIL
                     AEGRRFKMIRGMGSLAAMQDGSADRYFQEGEMTSTKMVPEGIEGRVPYKGPVGDVLFQ
                     MVGGLRSGMGYVGVADIPALQCEVELIRVTSAGLRESHPHDVTITREAPNYSA"
     gene            756..2240
                     /locus_tag="VFB89_12250"
     CDS             756..2240
                     /locus_tag="VFB89_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZM28125.1"
                     /translation="MSLFRRKDVASLQAEVARDHSLKRALGPVNLVFLGIGAIIGAGI
                     FVLTGQAAAQYAGPAIVWSFVLAGTACALAGLCYAEFSSMIPIAGSAYTYGYATLGEL
                     FAWIIGWDLILEYLFAASTVAVGWSGYVVSFLRDLGINFPKEFTSAPYTHTAPPDAGW
                     NVWRLFTEGWSSTGAVLNVPAMLIVAFITILLIIGITESATFNDAIVVIKVTVVLLFI
                     AFGLAYINRENWTPFIPPQEGPGKYGWSGIVRGAGVIFFAYIGFDAVSTAAQEAKKPQ
                     RDMPVGILGSLAICTVLYIAVALVLTGIVKFTQLNVPDPIAVGVNAAGPGLAWLRPIV
                     KIGAICGLTSVILVMLLGQPRIFYSMSKDGLLPPVFAAVHPKFRTPWLASLLTGVFAM
                     AMAGLLPIGLLGELVSIGTLLAFAIVCGGVLVLRYTDPDLPRPFRTPLVPLVPLGGIA
                     ACVYLMVHLPPDTWARLIIWMLIGFAIYFLYGRRHSKVGNQRTA"
     gene            2327..3349
                     /locus_tag="VFB89_12255"
     CDS             2327..3349
                     /locus_tag="VFB89_12255"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HZM28126.1"
                     /translation="MPYTLTNADREAAASLAAALSPGQRVCITTHVNPDGDGLGSEAG
                     LAHLLRAAGHDVAITNPSPTPSRFRFLLDDLPGVDRTGEAVKELRRADLIIILDISDL
                     GRLGMLAETVRDRGVPVACIDHHVSPGSLPPGPRYLDPTAAATGELVFELAVANGWPI
                     TQAAARGLYVALLTDTGGFRFSNTHPRTLRIAAELLEAGVDPEQIYLDVYARAPEGRP
                     RLFAEALQTLVVEPEVGLAWVTVPPGAIERLGVSSDDLDGVVEFPRSIEGVRMALLFR
                     EVSQGRVKVSLRSVGSVDVASFARRFGGGGHTKAAGLALPGNLAEVQETVLAAARAYL
                     GEDGRV"
BASE COUNT          533 a         1090 c         1065 g          695 t
ORIGIN      
        1 gctggtcgcg gggaacgtgg ccaccgaggc cggcgcgagg gagctcgtgc gccgcggcgc
       61 ggatgcggtg aaggtcggca tcggaccagg cagcatctgc acgacccgcg tcgtcaccgg
      121 cgtgggcgtg ccgcaggtaa ctgccatcat ggacgcggtg cgcggcgccg gcgacgtgcc
      181 ggtcatcgcc gatggcggca tcaagtattc cggcgatgtc gtgaaagcga tcgcggccgg
      241 agcgtcctct gccatgatgg gatccatgct cgccggcacc gaggaaagcc ctggcgaagc
      301 gatcctggcc gaaggccgcc gcttcaagat gatccggggc atgggcagtc tggctgcgat
      361 gcaggacgga tctgccgatc ggtatttcca ggaaggggag atgacgtcca ccaagatggt
      421 gcccgaggga atcgagggtc gggtacccta caagggtccg gtgggcgacg tcctattcca
      481 gatggtggga ggcttgagga gcggcatggg atacgtcggc gtagccgaca ttccggccct
      541 ccaatgcgag gtcgagctga tccgggtcac atcggccggc ctccgcgaat cccaccccca
      601 tgatgtgacc attactcggg aagcgccaaa ctacagcgct tgaaaagcgt aaacggtaaa
      661 cggtaaacgg tgaacgggag tttgcgttta cctgaccccc gttcaccgtt cactgttcac
      721 cgttcacgca gtctccctct caacccggac aaccgatgag tctcttccgc cgcaaggatg
      781 tcgcgagctt gcaggccgag gtggcccgcg accacagctt gaagcgcgct cttggccccg
      841 tcaacctcgt cttcttgggt atcggtgcca tcatcggcgc cggcatcttc gtgctgaccg
      901 gccaggctgc cgcgcaatat gccggtcccg cgatcgtgtg gtcattcgtg ctggcgggaa
      961 ccgcgtgcgc gctggccggc ctgtgttacg ccgagttttc gtcgatgatc ccgatcgctg
     1021 gctcggcgta cacctacggg tacgcgactc tgggtgaatt gtttgcgtgg atcatcgggt
     1081 gggacctgat tctcgagtat ctgtttgcgg cttcgaccgt cgccgtgggg tggtcgggct
     1141 atgtggtatc gttcctccgc gacctcggca tcaattttcc caaagaattc acctccgccc
     1201 cctataccca taccgcgcca ccggacgcgg gctggaatgt atggcggctc tttaccgaag
     1261 ggtggtcatc caccggtgcc gtgctgaacg taccggcgat gctcatcgtc gccttcatca
     1321 cgatcctgct catcattggc atcaccgaat cggcgacgtt caacgacgcc atcgtggtca
     1381 tcaaggtcac ggtcgtcctg ctctttattg cgtttggact tgcttatatc aaccgggaga
     1441 actggacccc gttcatcccc ccgcaggagg gccccgggaa gtatggctgg agcgggatcg
     1501 tacgaggcgc gggagtgatt ttcttcgcct acataggctt cgatgcggta tccactgccg
     1561 cccaggaggc caagaaacct caacgcgaca tgcctgtggg cattcttgga tcgctggcga
     1621 tctgtacggt gctctacatc gccgtggcgc tggtgctcac cggcatcgtg aagtttactc
     1681 agctcaacgt tcccgatccg atcgcggtgg gcgtcaacgc agccggtccc ggcctcgcct
     1741 ggctccgccc catcgtgaag atcggcgcca tctgcgggct cacgtccgtc atcctggtca
     1801 tgttgctggg ccagccccgc atcttctact cgatgtcgaa agacggattg ctgcctccgg
     1861 tgtttgcggc cgtgcatccc aagtttcgga ccccctggct cgcgagcctg ctcaccggcg
     1921 tttttgcgat ggccatggcc ggcttgctgc cgatcggtct cctgggcgag ctggtctcga
     1981 tcgggacgct gctcgctttc gccattgttt gcggcggcgt gctcgtactc cgatacaccg
     2041 acccggatct tcccaggcca ttccgcacgc cactggtccc gctcgtgccg ctcggcggca
     2101 tcgccgcgtg tgtttacctg atggtccacc tgccgcccga cacctgggcc cggctcatta
     2161 tctggatgct gatcgggttc gcgatctact tcctctacgg gaggcggcat tccaaggtgg
     2221 gcaaccagag gacagcgtaa acggtgagcg gtaaacggta aacgggggcc tccattacct
     2281 ctgagccccc gttcacggtt cactgttcac cgtttactgt tgtttcatgc cctacacgct
     2341 gacgaatgcc gatcgcgaag cagcagcgag cctagccgcg gccctgagtc ccggccagcg
     2401 ggtctgcatc accacccacg tcaatcccga cggtgacggt ctcggcagcg aggcgggtct
     2461 cgctcacctc ctgcgcgccg cggggcacga cgttgcgata accaatccgt ctccgacgcc
     2521 ctcccgcttc cgttttctgc tcgacgacct tcccggcgtg gaccgcaccg gggaggcggt
     2581 gaaagagctt cgtcgggcgg acttgatcat catcctcgac atttccgatc tcggccgcct
     2641 cggcatgctc gccgagaccg tccgggatcg cggcgtcccg gttgcatgca tcgatcacca
     2701 tgtgagcccg ggctcgttgc cgcccgggcc gcgttacctc gacccgaccg ccgccgccac
     2761 cggcgagctt gtgttcgagc ttgcggtcgc aaacggatgg ccgatcaccc aagccgctgc
     2821 ccgcggattg tacgttgcgc tcctcacgga tacgggtggg ttccggttca gcaataccca
     2881 tccccggact ctccgcatcg ccgctgagct tctggaggcc ggcgtcgatc ccgagcagat
     2941 ctatctggac gtgtacgctc gcgcgcccga gggtcggccc cgcttgtttg ccgaagcgct
     3001 gcaaacgctc gtagtggaac ctgaggtcgg gcttgcctgg gttaccgtac caccgggcgc
     3061 catcgaacgc ctcggcgtct cctctgacga tctcgacggg gtggtcgagt ttcctcgctc
     3121 catcgaaggc gtacgcatgg cgctgctgtt ccgtgaggtg tcgcaggggc gagtgaaagt
     3181 gtcgctccgc tcggtcggca gcgtagatgt cgcgtctttc gcgcggcggt ttggcggtgg
     3241 tggacacacc aaagctgcgg gcctggcatt gcccggcaac ttggcggaag tgcaggagac
     3301 tgttctagca gcggccagag cctacctcgg cgaggatggt cgggtttgat ccctgtgagt
     3361 cgcctcttat attgccgaat ccg
//
LOCUS       DASRJQ010000537         5062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1202504, whole genome shotgun sequence.
ACCESSION   DASRJQ010000537 DASRJQ010000000
VERSION     DASRJQ010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202504"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..2727)
                     /locus_tag="VFB89_12260"
     CDS             complement(40..2727)
                     /locus_tag="VFB89_12260"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZM28127.1"
                     /translation="MNRALELEPAARAAWLAEVRSEQPTVAAKLEALLAREAELDAHG
                     FLENAELIELLALSPPLAGRRVGAYTIERQLGQGGMGTVWLARRSDGRYEGKAAVKLL
                     NLALVDPVGSARFRREGTALARLTHPNIARLIDAGVTEEGQPFLVLEYVEGCRIDAYC
                     NQERLPPEQRLELFLQALDAVAHAHANLIVHRDIKPSNILVTGDGGVKLLDFGIAKLL
                     ESEAGSGEHSVLTELGGVALTPEYAAPEQVTGGAVTTATDVYALGVLLYILLSGRHPT
                     GEGCRSSAEHLRAIADTDPLRLSAAAATSERLRRLYAGDLDNIVAKVLRKDPAERYPT
                     VQAFGEDLRRYLRHEPVSARRDTVQYRLGKFVRRNRTSVTLGVLVLAALLAGLAGTIA
                     EARAAAREAHAAEGERDFALRALSRAQTVNELNHFLLKDAAPWGKPFTVGDLLARATR
                     LLDHQRAESDADRVEMLTAIGREYGTLDQSARARELLERAYAQSRTLADPSTRANAAC
                     NLAPALSEAGERVRAEQLIKEGLDELPDEPQFALDRITCLWLGTEVSIDNGSMESVPR
                     ARAALALFQHVPFPPAALEPSLLENLAEAYRVSGQFGLAVKAYGRASDQLTALGRDST
                     QAAGTVYNDWALSLTQIGQPLKAESLFRKAVAISSADQSGRSVSPVLLTNLARSLLEL
                     ERLPEAEEVAERAYGDARRAGDEATVNQCLLVRAAIYREQGALARADAMLAEVEPRLR
                     RMLPPGHYAFARVGVERALLAEARGDIAGAGASLDRALEVLEANVRTHQQGGFARADV
                     LRHRADLNLKLGRLAEAQHDAEQSLELLNEQQGPGLLSANVGRAYLSLGQALEAQGRT
                     SDAQRAFAAAVAQLEPTLGPEHPVTGQARALAALPTALH"
     gene            complement(2797..3360)
                     /locus_tag="VFB89_12265"
     CDS             complement(2797..3360)
                     /locus_tag="VFB89_12265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HZM28128.1"
                     /translation="MNTAIGELIRTAEAGNGAAANELFATLYRELHTIAERQLRQGGS
                     DLSLGTTTLLHEAYLDIAAREGLHFVDRPHFLAYASRAMRGLMIDYVRRRRAKKRGRE
                     FQIVQLHEQPDGSGDAPMTEPLEQLADALELVGEVDPALAQLVDLHFFCGFSFVEIAA
                     LREVSERTVRRDWRKARLLLHGALQEP"
     gene            3570..4646
                     /locus_tag="VFB89_12270"
     CDS             3570..4646
                     /locus_tag="VFB89_12270"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZM28129.1"
                     /translation="MLSRLSVCTALALLGCSKSGDRERQDTADGPATAAAPQKPAPFD
                     KGPVKIALVQYSGAGDYFEQWTKGARQQADSIGFEMQLHDAQADDAKQAADMKAAIAS
                     GVTGIIVDHGRSESLCPLINQATDGGIAVVVYDVKVLECAPKAVETKQNDADLAGLVL
                     TQMAKDIGDGVPVGYVNPFVIAPLERRDVVWKKFVADHKWKQKFAVGKFSHEVAKDNA
                     KLADRALKANPGVKAIFAPYDELTKGTVSAIEQNKLGTKVAAYGIDISNADIDVMTRK
                     DSPWKATATTDPSAVGAAVTRTLALELSGQLGKKEVMFPGVLVTQSYLVENQIKNMGD
                     LRAKLPELNLGTIASAPWIASIKF"
     gene            complement(4672..>5062)
                     /locus_tag="VFB89_12275"
     CDS             complement(4672..>5062)
                     /locus_tag="VFB89_12275"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HZM28130.1"
                     /translation="WGIKPWNGWWGKNPLFHTPVFVLTHHTRAPLEMEGGTRFIFVTD
                     GIETALAQAKKAAGKKDVSLGGGANVAQQYMAAGLIDEMELHVVPQLLGGGERLFKNL
                     DDAKVRLDPTRTVEGPGVTHLKYRVLK"
BASE COUNT          881 a         1717 c         1623 g          841 t
ORIGIN      
        1 gcaacgccgt ctgcgcgcag actggtactg gtcgcgcctc tagtgcagcg cggtcggtag
       61 cgccgcgagc gctcgtgcct gacccgtgac cggatgctcc ggacccaacg tcggctcgag
      121 ctgcgccacc gcggctgcga acgcgcgctg cgcatcgctg gtgcggccct gagcctcgag
      181 ggcctggccc aggctcaagt acgcccgccc gacgttcgcc gacaacagcc cggggccctg
      241 ctgctcattg agcagctcga gcgactgctc ggcgtcatgc tgcgcttcgg cgagccggcc
      301 cagcttgagg ttgaggtcgg ctcggtgccg caatacgtcg gcccgcgcga agccgccctg
      361 ctgatgggtt ctcacgtttg cctcgagaac ctctagtgcc cgatctagtg aggctcctgc
      421 accagcgata tcaccgcggg cctcggcgag gagagcgcgc tctacgccaa ctcgtgcgaa
      481 cgcatagtga cccggcggca gcatccgcct gagccggggc tcgacttccg ccagcatcgc
      541 gtccgcccgg gcgagcgcac cctgctctcg ataaatcgct gcgcgcacca gcaggcactg
      601 gttgaccgtg gcctcgtcgc ccgcacgccg ggcgtcccca taggccctct ccgccacctc
      661 ttccgcttca ggtagccgct ccaactcgag cagggacctt gcgaggtttg tcaaaaggac
      721 gggcgacacg gagcgtccgc tttgatccgc gctcgagatc gccaccgctt ttcgaaacag
      781 cgattcggcc ttgagcggct gcccaatctg ggtcagcgac aacgcccagt cgttgtagac
      841 ggtgcccgca gcctgggtac tgtcccgtcc cagggccgtg agctgatccg acgcgcgccc
      901 gtacgccttt accgccaggc cgaactgccc ggagactcgg taggcttcgg cgagattttc
      961 cagcagcgat ggctcgagcg ctgccggcgg aaacggaacg tgctggaaga gtgccagtgc
     1021 cgctcgagcc cgtggaacgc tctccatgct gccgttatcg atcgaaacct ctgtcccgag
     1081 ccagagacag gtaatccgat ccagcgcgaa ctgaggctca tctggaagct cgtccagccc
     1141 ctccttgatg agttgctcag ccctgacacg ctcgcccgct tcgctcagcg caggcgccag
     1201 gttgcaggca gcattcgccc gggtcgaggg gtccgctagc gtgcgggact gggcataggc
     1261 gcgttccagc agctcacggg ctcgagcgct ctggtcgagc gtaccgtatt cacggccaat
     1321 cgcggtgagc atttctactc gatcagcatc ggattccgcg cgttggtggt cgagcagcct
     1381 ggtggcccgc gcgaggaggt cgccaacggt aaacggcttc ccccatggcg ccgcgtcctt
     1441 cagcagaaaa tggttcagct cgttgacagt ctgtgcccgc gagagcgcgc ggagcgcgaa
     1501 gtcccgctct ccttctgcgg cgtgggcctc acgcgccgcc gcccgcgcct cggcaatcgt
     1561 gcccgccaga cccgcgagca acgctgccag cacgagcacg ccgagcgtta ccgatgtccg
     1621 gttccggcgg acgaacttcc cgagccgata ttgcaccgta tcgcgccgcg cgctgaccgg
     1681 ctcgtgcctc agataacgcc gcaggtcctc gccaaaagcc tgcaccgtcg gataccgctc
     1741 ggcgggatcc ttcctgagca ccttagcgac gatgttgtcg agatcgcccg catacaggcg
     1801 ccgcaaccgc tcggaggtgg cggcggcggc gctcagccgg agcggatctg tatccgcgat
     1861 cgcgcgaagg tgctcggcgc tcgagcggca cccttcgcca gtcgggtgcc gcccggaaag
     1921 gaggatgtac aacagcacgc ccagcgcgta gacgtcggtg gcggtcgtga cagccccacc
     1981 cgtcacctgc tccggcgccg cgtactcggg cgtgagcgcg acccctccca gttccgtgag
     2041 cactgagtgc tcccctgagc ccgcctccga ctccagcagc ttggcgattc cgaagtcgag
     2101 cagcttcacg ccgccatctc ccgtgaccag gatgttggac ggcttgatat cgcggtgaac
     2161 gatcagattc gcgtgcgcat gggcgacggc atccagcgct tgcaggaaca gctcgagccg
     2221 ctgctccggg ggaagacgct cctggttgca ataggcgtcg atgcgacaac cctcgacgta
     2281 ctcgaggacc aggaacggct ggccctcctc cgtcactccg gcatcgatca ggcgcgcaat
     2341 gttgggatgg gtcaggcggg cgagcgcggt gccctcccgg cggaatcgag cactgcccac
     2401 tgggtcgacc aacgcgaggt tcaacagctt gactgccgcc ttgccctcgt agcgcccgtc
     2461 gcttcgtcga gccagccaga ccgtacccat gccaccctgt ccgagctggc gctcgatggt
     2521 gtacgcaccc actcggcggc cggcgagcgg tggtgacaga gcgagcagct cgatcaactc
     2581 ggcgttttcc aggaagccgt gtgcgtcgag ctcagcttct cgggcgagca gtgcttcgag
     2641 ctttgcggcc acggtcggct gttcgctccg cacttccgca agccacgcag cccgtgcggc
     2701 cggctcgagc tcaagagcgc gattcacgag gggatgtaac cgagcgagca actccttcga
     2761 cataggggtc ataaggggtc tgccggcagc cgcggcctag ggctcctgga gtgcgccgtg
     2821 gagcaacagc cgagccttgc gccagtcccg gcgaacggtg cgctcggaga cctcgcgcag
     2881 ggccgcaatc tctacgaacg agaacccgca aaagaagtga aggtcgacca gctgggcgag
     2941 ggctggatca acctcgccga ccagttcgag cgcgtcggca agctgctcca gcggctcagt
     3001 catcggggca tcgccgctgc cgtccggctg ctcatgaagt tggacgatct ggaattccct
     3061 gccgcgcttc ttggcccgcc gccgacggac gtagtcgatc atgagcccgc gcatcgcgcg
     3121 cgaggcgtag gccagaaagt ggggccgatc gacgaaatgg agaccctcgc gcgcggcgat
     3181 gtcgaggtac gcctcatgga ggagggtcgt cgtgccgagg ctcaggtccg agccgccttg
     3241 ccgaagctgg cgctcggcaa tcgtgtgcag ctcacggtac agcgtggcga acaactcgtt
     3301 cgccgccgcc ccgttgcctg cctcggctgt gcggatcaac tccccgattg cggtgttcac
     3361 gcgcgggcat ctcttgaagc gccgccaccg gaaccggtgg ctcgacagag aacatacggt
     3421 ccgcttaagg acaggtcaaa gtgatctagc ccgccgttct gctcaccatg gacccgcccc
     3481 ctggcgcttt ctaagggcag gcggcatatg ctgtctaccg tcagcagaca ccagatcccc
     3541 caaaaacagg cgggaccatg agcagccata tgctttccag attgagtgtg tgcaccgcgc
     3601 tcgctcttct cgggtgctcc aagagcggcg accgtgagcg gcaggatacg gccgacggcc
     3661 ctgcaacggc cgcggcgccg cagaagcccg cgcccttcga taagggcccg gtgaagatcg
     3721 ccctggtgca gtacagcggc gcgggcgatt acttcgagca gtggaccaaa ggcgcgcggc
     3781 agcaggccga ctccatcggc ttcgagatgc agctccacga cgcgcaggcc gatgacgcca
     3841 agcaggccgc cgacatgaaa gccgcgatcg cgtccggagt cacaggcatc attgtggacc
     3901 atggccggtc cgagagcctg tgcccgctga tcaaccaggc gaccgacggc gggatcgccg
     3961 tagtggtgta cgacgtgaag gtgctcgagt gcgctcccaa ggcggtcgag accaagcaga
     4021 acgatgcaga tctcgccggc ctggtgctca cccagatggc gaaggacatt ggtgatggcg
     4081 ttccggttgg ctatgtcaac ccgttcgtca tcgcaccgct cgagcggcgg gatgtggtct
     4141 ggaagaagtt cgtggcggac cataagtgga agcagaagtt tgccgtgggc aagttcagcc
     4201 acgaagtcgc gaaggacaac gcaaagcttg ccgaccgcgc gctcaaggcg aaccctggcg
     4261 tgaaggcgat ctttgctccc tacgacgagc tcaccaaagg caccgtctcg gccatcgagc
     4321 agaacaagct gggcaccaag gtcgccgcat acgggatcga catctcgaat gccgacatcg
     4381 acgtgatgac caggaaggac agtccctgga aggcgaccgc caccaccgac ccgagcgccg
     4441 tcggcgccgc cgtcacccgg acgctggccc tcgagttatc cggtcagctt ggcaagaagg
     4501 aagtgatgtt ccccggtgtc ctggtcaccc agtcgtatct cgtggagaac cagatcaaga
     4561 acatgggcga cctgcgggcc aagctgcccg agctgaacct tggcacgatc gcctcggcgc
     4621 cgtggattgc gtcgatcaag ttctgactac aacgtcccgc gaagcgatgg cctacttgag
     4681 cactcgatac ttgaggtggg tcactcccgg cccctcgacg gtgcgagtcg ggtcgagcct
     4741 caccttcgca tcgtcgaggt tcttgaacag tcgctcacct ccgccgagga gctgaggcac
     4801 gacgtgcagc tccatctcgt caatcaggcc cgcggccata tactgctgcg cgacgttggc
     4861 tccgccgccg agcgacacgt ccttcttgcc ggcagccttc ttcgcctggg cgagggcggt
     4921 ctcgatcccg tcggtcacga agatgaaccg cgtcccgccc tccatctcga gcggcgcgcg
     4981 ggtgtgatgg gtcaacacga acaccggcgt gtggaacagc gggttcttcc cccaccagcc
     5041 gttccacggc ttgatccccc ag
//
LOCUS       DASRJQ010000538         6261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_358146, whole genome shotgun sequence.
ACCESSION   DASRJQ010000538 DASRJQ010000000
VERSION     DASRJQ010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358146"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2396)
                     /locus_tag="VFB89_12280"
     CDS             complement(<1..2396)
                     /locus_tag="VFB89_12280"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HZM28131.1"
                     /translation="MTQESPHSYLVTELPASSDPLAESAAVHATEVADFLFNQLLAVV
                     RRRNPELAPLLAGEAERCAPELETGTLQVLGIWLQLLSIAEQNAAMRRRRQIEAERGY
                     EALRGTLMQTVGEAAGAGVKADELQERLRSLRVRPVLTAHPTEAKRVTVLEKHRRIYR
                     LLVELESPRWTPRERAQLQRQVGFEIELLWLTGELRLEKPTVEQEVFWALHFFNETLF
                     ESVPQIHDRLERALAEHYPDQSFEVPPFFQFGSWVGGDRDGNPFVTNEVTRRSLLEYR
                     LAALRRYRQRLGDLIRRLSISERGVRVSDEFRRHLDRLLAASADAAGIASRNPGEIFR
                     QLLACVLRRLDLALASAERHEIGSSALTADELAADLRMLERGLTEAVDPELAMSLVRP
                     VRREVELFRFSTVRLDIRENTTKLNEALGALWQLRNQQPGAAVPDPKSKEWCSWIQAE
                     LGRPRSGVLQLSDLPDSAAETIGMFRLVAELRDQVDRDAFGSFILSMTHSVTDVLGAY
                     LAAKEGGLFPDTAAVERCVLPIVPLFETIDDLRRAPAIMRELLNVPVVRRSIREQGGV
                     QEVMIGYSDSNKDGGYFAANWELYKAQVKLTRLGEELGIPIAFFHGRGGSVSRGGAPT
                     GRAIAAQPAGSINGRFRLTEQGEVVSFKYANRGTAAYQIELLASAVLEHSLKSQREQA
                     LVPVGEFDEAMEAIAGPSMAAYRQLIDHPMLLSYYQVASPLEEISLLNLGSRPARRFG
                     ARTIGDLRAIPWVFAWTQNRHFVPSWFGMGSGLETFMQVRGPRGEALLRRMFRDSRLF
                     R"
     gene            complement(2393..3364)
                     /locus_tag="VFB89_12285"
     CDS             complement(2393..3364)
                     /locus_tag="VFB89_12285"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HZM28132.1"
                     /translation="MSQPVVVVTRKLPGAVEEAVKREFDARLNREDRPLSTGELQAAL
                     RSADALLPTVTDKLTADVLSAEPLRTKMIANFGVGFNNIDTEAAKARGIAVSNTPDVL
                     TDATADLAFTLMLMIARRAGEGERHVRSSHWTGWRPTHMLGRMVAGKTLGLIGMGRIA
                     RAVAQRAHHGFGMRVLYTDPYPPPPDVAAALGAEQRATIEEVLAQSDFVSLHCPATPE
                     THHLMSSVRLAKMKPSAYLINTARGDVVDEAALVEALKKGWIAGAGLDVYEREPQIAS
                     ALVAMENVVLLPHLGSATEETRVAMGLRALENLRLFFSGAPLRDRVA"
     gene            complement(3361..4272)
                     /gene="sucD"
                     /locus_tag="VFB89_12290"
     CDS             complement(3361..4272)
                     /gene="sucD"
                     /locus_tag="VFB89_12290"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HZM28133.1"
                     /translation="MSILLDEKTRVIIQGFTGDKGTFHGREMLAYGTNLVGGVTPGKG
                     GTSHLDRPVFNTVKEAVRATGAEASLIFVPAAFCADSIMEAADAGIRLVVAITDGIPA
                     QDMMMVKRYLFRYARDKKTMLVGPNCAGIISPGKAMLGIMPGHIYMKGKVGLVTRSGT
                     LGYEAASQMKALGIGVSTSIGIGGDPINGSSFVDVLRLFAEDPETEAVMMIGEIGGPQ
                     EAEAARFFRENGRKPVIGYVAGLSAPKGRRMGHAGAIIRAFGESAAEKVEIMREAGIT
                     VAASPAELGSTVASVLGSISKRVAVPT"
     gene            complement(4269..5462)
                     /locus_tag="VFB89_12295"
     CDS             complement(4269..5462)
                     /locus_tag="VFB89_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate--CoA ligase subunit beta"
                     /protein_id="HZM28134.1"
                     /translation="MDIHEYQAKELLAGFGVAIPRGGVAYSPEQAVYRATEIGGNRWA
                     VKAQIHSGARGKAGGIKVCTSEDEVRQAAKGLLGKRLVTHQTGPEGRVVHRLYVEAAV
                     PIERELYIGFVLDRKSERVMIVAHPQGGMEIEEISQTAPDTIIRAVVEPAVGMTPFQA
                     REIAFALRLDAAQVSRGATTLLGCYRAFRDLDATMVEINPLVLTKDGQLLALDAKMTF
                     DDNALFRRPNVSELRDYAEEDPRESQASEYGINYVALDGEIGCIVNGAGLAMATMDLI
                     QHAGSSPANFLDVGGGASPEKVTNAFRLVLADPKVKAILVNIFAGINRCDWVAQGVVQ
                     AVRELRPPVPLVVRLAGTNVEEGRRILNDSGLQLMTADTLTEAAERVVTALKATGRKA
                     GNGKA"
     gene            complement(5515..>6261)
                     /locus_tag="VFB89_12300"
     CDS             complement(5515..>6261)
                     /locus_tag="VFB89_12300"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HZM28135.1"
                     /translation="GRGKTISVRINGIDTHYMYRDVVDVVEQAGHRLDTVLIPKVGVP
                     ADVYLVDALLTQIEAAKRIPYRLGIEVLIETALGMANVEAIAATRGRLEVMHFGVADY
                     AASNRARTVSIGGLNPDYPGDQWHFGLSRMTVACRAYGLRPIDGPFGDFKDADGYRAA
                     ARRGAALGIEGKWAIHPSQIALANDVFTPPEAEVSRARRILDALEAAARDGKGAAQLD
                     GKMIDAASARMAEGIVRMAEAIGKKKLADS"
BASE COUNT         1067 a         2135 c         1923 g         1136 t
ORIGIN      
        1 cggaagagtc gcgagtcgcg gaacatccgc cgcagcagcg cttcgccccg tggcccccgg
       61 acctgcatga aggtctcgag cccgcttccc atcccgaacc agctgggcac gaagtgacga
      121 ttctgagtcc aggcgaacac ccaggggatg gctcgtaagt cgccgatcgt tcgagcgccg
      181 aacctacgag caggccgcga gccgaggttg agaagggaga tctcctccag gggactcgcg
      241 acctgatagt aggagagcag catgggatga tcgatcagct gccggtacgc agccatcgaa
      301 ggaccggcga tggcctccat cgcttcgtcg aactcgccca cggggaccag cgcctgctcc
      361 cgctgcgact tgagcgaatg ctcgagcacg gcggacgcca gcagctcgat ctggtacgcg
      421 gccgtcccgc ggttggcata cttgaacgag actacttcac cttgctcggt caggcggaat
      481 cgtccgttga tcgaaccagc gggctgcgcc gcaattgccc ggccggtggg tgcgccgccc
      541 cggctcaccg atccaccccg gccgtgaaag aacgcgatcg ggattccgag ctcctcgccc
      601 agccgggtga gcttcacctg tgccttgtac agttcccaat tagcagcgaa gtagccgccg
      661 tccttgttgg agtctgaata accgatcatc acctcctgca ccccgccctg ctcgcggatg
      721 ctccgtctca cgacggggac gttcaagagc tcgcgcatga tcgcaggcgc ccgcctgagg
      781 tcgtcgattg tctcgaacag cggcacgatg ggaaggacgc atcgctcgac tgcggccgta
      841 tccggaaaga ggccaccctc cttggccgcg agatacgcgc ccagcacatc agtgaccgag
      901 tgggtcatgc tgaggatgaa gctcccgaac gcatctcgat ccacctggtc gcgcagctcg
      961 gcaaccagcc ggaacatgcc gatcgtctcg gccgcggagt ccggcagatc cgacagctgc
     1021 aatacacctg agcgaggacg ccccagctcg gcctgaatcc agctgcacca ttctttcgac
     1081 ttgggatccg gaaccgcggc ccccggttgc tgatttctga gctgccaaag cgcgcccaga
     1141 gcctcgttca gtttcgtggt gttctcacga atgtcgagcc gcacggtact gaaccggaac
     1201 agctcgactt cacgacgcac cgggcggacc agactcatag ccaactcggg atcaaccgct
     1261 tcggtaagtc cacgctcgag catccggagg tcggccgcca gctcgtccgc cgtcagagcg
     1321 ctgctgccga tttcatgacg ttcggcggac gccagggcga ggtcgaggcg ccgcagaacg
     1381 caggcgagca gctgccggaa gatctcccca ggatttcggc ttgcgatccc ggccgcgtcg
     1441 gcgctcgcag caagtagccg atccagatgc cggcgaaact cgtccgagac ccggacgccg
     1501 cgttcgctga tgctcaggcg gcgaatcaga tcccccagtc gctggcgata ccgccgcagc
     1561 gcggcgagcc ggtactcgag aagcgaacgg cgggtcactt cgttggtgac gaagggattc
     1621 ccatcccggt cgccgcctac ccaggagccg aactgaaaaa agggagggac ctcgaaggac
     1681 tggtcgggat agtgttcggc cagcgcgcgc tccaggcggt cgtgaatctg agggaccgac
     1741 tcgaacaagg tctcgttgaa gaagtggagg gcccagaata cctcctgctc taccgtcggc
     1801 ttttccaggc gcagctcgcc ggtcagccag agcagctcga tctcgaagcc gacctgccgc
     1861 tgcaactgag cgcgctctcg cggagtccag cgcggcgact ccagttccac caggaggcgg
     1921 tagatccggc ggtgcttctc cagaacggtg acgcgcttcg cttcggtggg gtgagcggtc
     1981 agcacgggcc ggacccggag ggagcggagt cgttcctgaa gctcgtcagc tttcacgccc
     2041 gcgcctgccg cctctccgac ggtctgcatc agcgtgccgc ggagcgcttc gtagcctcgc
     2101 tccgcctcga tctgccggcg ccgtcgcatg gccgcgttct gctcggcgat gctcagcagc
     2161 tggagccaga tccccagaac ctggagagtc ccggtttcca gctccggcgc gcacctctca
     2221 gcctcaccgg cgagcaatgg cgccagctcg ggatttcgcc tccgcaccac cgccagcaac
     2281 tgattgaaca ggaaatcggc gacttcggta gcatggaccg cggcgctttc ggccagcggg
     2341 tccgaggagg cgggaagctc ggtgacgaga taggagtgag gtgattcctg ggtcacgcta
     2401 cgcgatcgcg caggggagcc cctgaaaaga agagtcgaag attttccagg gcccgcaggc
     2461 ccatggcgac gcgggtctct tccgtcgcac tccccaggtg cggcaggagc acgacgtttt
     2521 ccatggcgac cagcgccgaa gcaatctgag gttcacgctc gtagacgtcg aggcccgcgc
     2581 ccgcgatcca gcccttcttc aaggcttcca ccagcgctgc ttcgtccacc acgtcgcctc
     2641 gcgcggtgtt gatgaggtag gcgctgggtt tcatcttggc cagtcgcacg ctgctcatga
     2701 ggtgatgcgt ttcgggcgtg gcggggcaat gcagcgagac gaaatcgctc tgcgccagca
     2761 cctcttcgat agtggcacgc tgctcggcac ccagcgccgc agcgacgtcg ggcggcggcg
     2821 gataaggatc ggtgtatagg acgcgcatgc cgaatccgtg atgggcccgc tgggccaccg
     2881 cccgggcaat tcgccccatg ccgatcaggc cgagcgtctt gcccgcgacc atccggccga
     2941 gcatgtgcgt aggccgccag ccggtccagt gagaagagcg cacgtgacgc tcaccctcgc
     3001 ccgcgcgccg cgcaatcatg agcatcagcg tgaacgcaag atcggcagtc gcgtcggtga
     3061 gcacgtccgg cgtgttcgac accgcgatcc cgcgagcctt ggccgcctcg gtatcgatat
     3121 tgttgaagcc gacgccgaag ttggcgatca tcttggtcct cagaggctcg gcgctcaaca
     3181 cgtcagcggt gagcttgtcg gtcaccgtag gcagcagcgc atccgcgctc cggagcgccg
     3241 cctgcagctc cccggtgctg agcggccgat cctcccggtt caggcgggca tcaaactctc
     3301 tcttgactgc ttcctcgacc gcgccgggca gcttacgcgt cacgacgacg actggctggc
     3361 tcacgtgggt accgccacgc gcttggagat cgagcccagt accgatgcga cggtcgagcc
     3421 cagctctgcc ggactcgcgg caacagttat cccggcctcg cgcatgatct cgactttctc
     3481 cgccgcactc tcgccgaacg cccgaatgat ggcgcctgca tggcccatcc gtcgtccttt
     3541 gggcgccgac aggcccgcga cgtaaccgat caccggcttc cggccgtttt cgcggaagaa
     3601 gcgagccgcc tccgcctcct gcggtccgcc gatctcgccg atcatcatga ccgcttcagt
     3661 ctccggatct tccgcgaaca agcggagcac atccacgaac gagctgccgt tgattggatc
     3721 gccgccgatc ccgatgctgg tagacacccc gatgccgagc gctttcatct gggacgccgc
     3781 ctcgtatccg agcgttccag agcgcgtgac cagacccacc ttccccttca tatagatgtg
     3841 acccggcatg atgcccagca tcgccttgcc cgggctgatg atgccggcac agttaggccc
     3901 gaccagcatg gttttcttgt cccgcgcgta ccggaacagg tagcgcttga ccatcatcat
     3961 gtcctgcgcc ggaatgccgt cggtgatcgc gacgaccaga cgaatccccg cgtcggctgc
     4021 ctccatgatg gaatcggcgc agaaggcggc cggaacgaag atcaggctcg cctcggcgcc
     4081 ggttgcgcga accgcctcct tcaccgtgtt gaagaccgga cggtccaggt ggctggtgcc
     4141 gcctttgccc ggcgtcacgc cgccgacgag gttggttccg taggcgagca tttcccggcc
     4201 gtgaaaggtg cctttgtcgc ccgtgaaacc ctggatgatg actcgggtct tttcgtcgag
     4261 gaggatgctc atgctttccc gttcccggcc tttcggcccg tggccttgag agcggtcacg
     4321 acccgctctg ccgcttcggt gagagtgtcc gctgtcatca gctgaagccc actgtcattc
     4381 agaatccggc gcccttcctc gacgttggtg cccgcgagtc ggaccaccag gggtaccggt
     4441 ggccggagct cccgtaccgc ctgcactacg ccttgcgcca cccagtcgca tcggttgatg
     4501 ccggcaaaga tgttcacgag aatcgccttc accttcggat ccgccagcac cagccgaaac
     4561 gcattcgtca ccttctccgg cgaggcgccg ccgcccacgt ccagaaaatt ggctggcgag
     4621 cttcctgcgt gctggatgag atccattgtc gccatcgcca atccggcccc gttcacgatg
     4681 cagccgatct cgccgtcgag cgctacgtag ttaatgccgt actcggacgc ttgcgactcg
     4741 cgcggatcct cctcggcgta gtcgcgcagc tcgctcacgt taggccgccg gaagagcgca
     4801 ttgtcgtcga aggtcatctt ggcgtcgagc gcgagcaact gaccgtcttt ggtcagcacg
     4861 agcggattga tctcgaccat tgtcgcatcg aggtcccgga acgcacggta gcatcccagc
     4921 agcgtcgtcg ctccccggct tacctgcgcc gcgtcgagcc gcagcgcaaa ggcaatttcc
     4981 cgggcctgga atggtgtcat gccgaccgcg ggctcgacga ctgcgcgaat aatcgtgtcc
     5041 ggggcggtct ggctgatttc ttcgatttcc attccaccct gcgggtgcgc gacgatcatg
     5101 acccgctcgc tctttcggtc gagcacgaag ccgatgtaca gctcacgctc gatggggacg
     5161 gccgcctcga cgtagaggcg atgcacgacg cggccctcag gcccggtctg atgggtgact
     5221 aagcgcttgc cgagcagccc cttcgcggcc tggcgtactt cgtcctcgct ggtgcagacc
     5281 ttgatgccgc cggcctttcc gcgggcacca gaatggatct gcgccttcac cgcccaccgg
     5341 ttgccgccga tctcggtggc gcgatacacg gcctgttcgg gactgtaggc gacgccgccc
     5401 cgcggaatcg cgaccccgaa acccgccagc agctcttttg cctggtactc gtggatgtcc
     5461 aagtctcttc tccgggtatt gagccattcg cggtctgcta tcagctatca gctatcagct
     5521 atcagctagc ttcttcttcc cgatggcttc cgccattctc acgatccctt ccgccatcct
     5581 cgccgacgcc gcgtcgatca tcttgccatc gagctgggcc gcgcctttgc catcgcgggc
     5641 cgcagcctcg agcgcatcca gaatgcgccg ggctcggctc acttcggctt ccggaggcgt
     5701 aaacacgtcg ttggccagcg cgatctgcga cgggtggatg gcccacttac cctcgatgcc
     5761 gagcgccgcg ccgcgccgtg ccgcggcacg atatccgtcc gcgtccttga agtcaccgaa
     5821 cggtccgtcg atcggtcgaa gcccgtatgc ccgacacgct acggtcatgc gactcaatcc
     5881 gaaatgccat tggtcgccgg ggtagtcagg gttgagaccg ccgatgctga cggtgcgcgc
     5941 ccgattgctg gcggcgtagt cggccacccc gaaatgcatg acctcgagcc ggcccctggt
     6001 tgctgcaatc gcctcgacgt tggccatgcc gagcgcggtc tcgatcagaa cctcgatacc
     6061 cagtcgataa ggtatccgtt tggccgcctc gatctgagtc agcagcgcgt cgaccagata
     6121 gacgtcggca ggcacgccca ccttcgggat caacacggtg tcgaggcggt gtccggcctg
     6181 ttccaccaca tccaccacat cacggtacat gtaatgggtg tcgatcccgt tgattcggac
     6241 agagattgtt ttcccccgtc c
//
LOCUS       DASRJQ010000539         3735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2191722, whole genome shotgun sequence.
ACCESSION   DASRJQ010000539 DASRJQ010000000
VERSION     DASRJQ010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2191722"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..673)
                     /locus_tag="VFB89_12305"
     CDS             complement(80..673)
                     /locus_tag="VFB89_12305"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HZM28136.1"
                     /translation="MKRSILAVAALVLVTAHPSLAADSKVKIYGMLAYVSPLAESDQT
                     VAGVTDAVKASSEIGYNFGLEFRASPLIGLEFDYLYATQEVTADKAGLLGETTFQPIS
                     GTLNLHVPLGMFDAYGGLTAAYVNWGDLKAPPPGVDVKVDPEFAYGISGGLDLNLTPN
                     LAATGGLRWLNLQAQPDGGEALDVNPLFTRLGIAWRF"
     gene            913..1266
                     /locus_tag="VFB89_12310"
     CDS             913..1266
                     /locus_tag="VFB89_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28137.1"
                     /translation="MKPIHAKTSAAAILLTAVLLSLPGCTQVGDRLTGVRLESHRPNE
                     CVKACNQEFVADLTAETKKLTQALEACRQSTDAERKACHQAALAVFHAAVTALGQERK
                     GCVNDCHQQGSGQGG"
     gene            1461..1706
                     /locus_tag="VFB89_12315"
     CDS             1461..1706
                     /locus_tag="VFB89_12315"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZM28138.1"
                     /translation="MDCGELLEQLSEYLDHEAKVELCREIEKHLEHCHDCSIKVDQTQ
                     KTILLYQANRQVDVPFAVSEKLRAALSSEYRGAASAD"
     gene            1799..2833
                     /locus_tag="VFB89_12320"
     CDS             1799..2833
                     /locus_tag="VFB89_12320"
                     /inference="COORDINATES: protein motif:HMM:NF017847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HZM28139.1"
                     /translation="MKRVGLLGLPQSGKSTVFEILLLGAGAAAPTSQGRESIGVVRVP
                     DSRLDQLSRLYQPKKTTYAQIQFVDTIATVAGSARSAAKGQDLFSAVRTCDALALVLR
                     DFEAGSEPGRDLATLEAELILNDLALVEGRIERIEKELRVGKRQNEREQVLLGRCKTL
                     LESEQPLRAQSFDADQDRTLRGFQLLTLKPLLVIVNRGDAESALAPEAGPGREVVTLR
                     ALLEREVLSLAPGERDGFRAELGIGEDGLSVVIRACYRLLGLQSFFTFGEDECRAWTV
                     RRGAKAVDAAGEIHSDLARGFIRAEVVPWDKLLEAGSEAKARERAWQRLEGRDYEVQD
                     GDCITVRFNK"
     gene            2841..3389
                     /locus_tag="VFB89_12325"
     CDS             2841..3389
                     /locus_tag="VFB89_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28140.1"
                     /translation="MRVLLLVAVAFLAARPAAALTLEQVGGHFGFGYGQLLNDDPPAG
                     SLAFGAGVDIPVLPSLRAGIDLGFDLLGSRVEERGSVSADVEYSMFEVFGLLHWTPRD
                     AWRLSMGPGLVHARGDLSSSGPVAFEDLAVNEAAPGGSLSVQWLMGSKSPLRVGVEAG
                     VRTAWMESETWTVMLGRLMVHY"
     gene            3476..>3735
                     /locus_tag="VFB89_12330"
     CDS             3476..>3735
                     /locus_tag="VFB89_12330"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM28141.1"
                     /translation="MSSLLGQAVSHYTVTRKLGSGGMGVVYEAEDSRLGRKVALKFLP
                     PDTEQDPATLERFQREARAASALNHPNICTVYAIEQHERQHF"
BASE COUNT          645 a         1198 c         1277 g          615 t
ORIGIN      
        1 agggtgtcgc tggttcgatc ccagcacggg tcaccacgct acgaccgctt cgcgcatgcc
       61 acgtcgggaa tgccccctct cagaacctcc aggcaatccc aagccgcgtg aacagcggat
      121 tcacgtccag cgcctcgccg ccgtccggct gcgcctggag gttcagccag cgcagaccgc
      181 cggttgcggc caggttcggc gtgaggttca ggtcgaggcc gcccgaaatg ccgtacgcga
      241 actcggggtc gaccttgaca tcgacaccgg ggggaggagc tttcagatct ccccagttga
      301 cgtaggccgc cgtcaaccct ccatacgcgt caaacatgcc cagagggacg tgcaggttga
      361 gcgtgccgct gatgggctgg aacgtcgtct cacccagcag ccctgccttg tcggcggtca
      421 cttcctgggt cgcgtacagg taatcgaact ccaggccgat cagcgggctg gcgcggaatt
      481 cgagcccgaa gttgtagccg atctccgacg aggctttgac cgcatccgta acgccagcga
      541 cggtctggtc ggactccgcc agcggtgaga catacgccag cattccatag atcttgacct
      601 tcgaatccgc cgccaacgag ggatgggcgg tcacgaggac gagtgcggct acggcgagga
      661 tggatctctt catctcgaac ctccttgctc tgtgcgtgcg gctccttcgg aaccaaaccg
      721 aaggctagcg acccggtcac taggatggca aacggcggcc ccgggtgccg tcgagggcgc
      781 ccccgcagtc agacggggcc aaaggcgggg cggggggggc caggaccagc cgagtgagct
      841 ttctacgggt gaattcgtgt agtctagggc gcgtccggca ggaagccggc gaactcatcc
      901 cagggaggga tcatgaagcc tatccacgcg aaaacctccg cggcggccat cctcctcacg
      961 gccgttctgc tgtcgctccc cggctgcacc caagtggggg atcggctcac cggcgtccga
     1021 ctcgagtccc accgccccaa cgagtgcgtc aaggcatgca accaggagtt cgtggccgac
     1081 ctgacggccg agaccaagaa actcacgcag gctctggagg cttgccgaca gagcaccgac
     1141 gcggaaagga aggcctgcca ccaggccgcg ttggcggtct tccacgcggc cgtgacggcg
     1201 ctcggccagg agcgaaaagg ctgcgtcaat gactgccacc agcagggctc cggccaaggc
     1261 ggctgattcg tcgttgatcg tcgcgtgccc ggctgtgcag taagtcgtcc gggttaccaa
     1321 aatagttttg ccccctgtca actttatgca aacctgactc gaggttgccg gcatcaacat
     1381 ggtccggcgc atcggattga gcggagccgc cccgcccggt gcgttcaaac ggggccggaa
     1441 aacaggggga acacagaccg gtggattgcg gcgagcttct agagcaattg agcgaatacc
     1501 tcgatcacga ggcgaaggtc gagctgtgcc gcgagatcga gaaacatctc gagcattgcc
     1561 acgattgcag catcaaggtg gaccaaacgc agaagaccat cctcctgtat caagcgaacc
     1621 gacaggtgga tgtgcctttt gcggtgagtg agaagcttcg ggcggcgctg tcctccgagt
     1681 accgcggcgc ggcgtcggcc gattgacact ccgaacgagc gccacctaaa ctgcgcggcc
     1741 gtcgacgccc gcggggacca tccccgcggg cggttcgcat gttaggggtg gagagttcat
     1801 gaagcgcgtc ggcctgctcg ggctccccca gtccggcaaa tccacggttt tcgagattct
     1861 gctcctgggc gccggggccg cagcccccac ctcccaagga cgcgagagca tcggcgtggt
     1921 gcgagtgccc gactcgcggc tcgaccagct ctcccggctg tatcagccca agaagaccac
     1981 ctacgcccag atccagttcg tggacacgat cgccaccgtc gccggcagcg cccgctcagc
     2041 ggccaagggg caggatctgt tctcggcggt ccgcacctgc gacgctctcg ccctggtgct
     2101 gcgcgacttc gaggcgggct cggagccggg ccgtgacctg gccacactcg aagccgagct
     2161 gatcctcaat gatctcgccc tggtcgaggg ccggatcgag cggatcgaga aggagcttcg
     2221 ggtaggcaag cgccagaacg agcgcgaaca agtgctgctc ggtcggtgta agaccttgct
     2281 cgagagcgag cagccgctgc gcgcgcagtc cttcgacgcc gatcaggacc gcaccctgcg
     2341 gggattccag ctgctgacgc tcaagccgct gctggtgatc gtcaaccggg gcgacgcgga
     2401 gtcggcgctg gcgcctgagg ctggaccggg gcgtgaggtg gtgacattgc gggcgctgct
     2461 ggagcgcgag gtgctgagtc tcgccccggg cgagcgcgat gggttccgcg ccgagctggg
     2521 gatcggcgaa gatgggctgt cggtggtgat tcgggcgtgc tatcggctgt tgggcctgca
     2581 gtcgttcttc acctttggag aggacgagtg ccgggcgtgg acggtgcgac gcggcgccaa
     2641 ggcggtggat gcggcaggtg agatccacag cgacctggct cgcggcttca ttcgcgccga
     2701 ggtggttccc tgggacaaac tgctggaagc cgggagcgaa gcgaaggccc gggagcgggc
     2761 atggcagagg ctggaggggc gggattatga ggtccaggac ggcgactgca tcacggtgcg
     2821 cttcaacaag tagatcgtcc gtgcgggtcc tcctgctggt ggcagtggcg ttcctcgccg
     2881 cccggcccgc cgcggccctg accctcgaac aggtgggcgg ccacttcggg ttcggctacg
     2941 gccagctcct caacgacgac cctccggctg gctcgctggc gttcggagcc ggggtcgaca
     3001 tacccgtcct gccctcgctg cgcgcgggca tcgacctcgg cttcgatttg ctcggcagcc
     3061 gggtcgagga gcgcggcagc gtctcggccg acgtggaata cagcatgttc gaagtgttcg
     3121 gcctgctcca ctggacgccc cgagacgcgt ggcggctctc catgggaccg ggcctggttc
     3181 acgcgcgtgg cgacctctcc tcgtccggtc cggtggcctt cgaggacctg gcggtgaacg
     3241 aggcggcacc cggcggctcg ttgagcgttc aatggttgat ggggagcaaa tcgccgctgc
     3301 gcgtgggcgt ggaggccgga gtgcgcaccg cctggatgga gagcgagacc tggacggtga
     3361 tgttgggtcg tctgatggtc cactactagg cggtctgaga cgccgcaggg cgcaacaaac
     3421 cgcgtcgttc cttgacgatc cggcaactgc cgacgtagca tccttgcttc cttccatgag
     3481 ctccctcctc ggccaggccg tttcccacta caccgtgacc cgcaagctgg gaagcggggg
     3541 catgggagtg gtctacgagg ccgaggactc gcggctggga cgcaaggtgg cgctcaagtt
     3601 cctgccgccc gacaccgagc aggaccccgc gacgctggag cgctttcagc gcgaagcgcg
     3661 ggcagcctcg gccctcaacc accccaacat ctgcaccgtg tatgcgatcg agcagcacga
     3721 gcgccagcac ttcat
//
LOCUS       DASRJQ010000540         3272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2042320, whole genome shotgun sequence.
ACCESSION   DASRJQ010000540 DASRJQ010000000
VERSION     DASRJQ010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2042320"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VFB89_12335"
     CDS             <1..186
                     /locus_tag="VFB89_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28142.1"
                     /translation="TGLVNIIVKPDQFERWKDVLVTSPVLEIHGFLQSQEGMAVRALR
                     ARPVETAQAVSRSRDFH"
     gene            528..602
                     /locus_tag="VFB89_12340"
     tRNA            528..602
                     /locus_tag="VFB89_12340"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:560..562,aa:Trp,seq:cca)
     gene            684..757
                     /locus_tag="VFB89_12345"
     tRNA            684..757
                     /locus_tag="VFB89_12345"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:716..718,aa:Gly,seq:gcc)
     gene            760..834
                     /locus_tag="VFB89_12350"
     tRNA            760..834
                     /locus_tag="VFB89_12350"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:792..794,aa:Gly,seq:tcc)
     gene            914..988
                     /locus_tag="VFB89_12355"
     tRNA            914..988
                     /locus_tag="VFB89_12355"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:946..948,aa:Lys,seq:ctt)
     gene            1133..1206
                     /locus_tag="VFB89_12360"
     tRNA            1133..1206
                     /locus_tag="VFB89_12360"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1165..1167,aa:Asp,seq:gtc)
     gene            1211..1284
                     /locus_tag="VFB89_12365"
     tRNA            1211..1284
                     /locus_tag="VFB89_12365"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1243..1245,aa:Ala,seq:cgc)
     gene            1297..1372
                     /locus_tag="VFB89_12370"
     tRNA            1297..1372
                     /locus_tag="VFB89_12370"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1330..1332,aa:Met,seq:cat)
     gene            1375..1448
                     /locus_tag="VFB89_12375"
     tRNA            1375..1448
                     /locus_tag="VFB89_12375"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1407..1409,aa:Val,seq:tac)
     gene            1459..1533
                     /locus_tag="VFB89_12380"
     tRNA            1459..1533
                     /locus_tag="VFB89_12380"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1492..1494,aa:His,seq:gtg)
     gene            1545..1620
                     /locus_tag="VFB89_12385"
     tRNA            1545..1620
                     /locus_tag="VFB89_12385"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1578..1580,aa:Pro,seq:cgg)
     gene            1755..1825
                     /locus_tag="VFB89_12390"
     tRNA            1755..1825
                     /locus_tag="VFB89_12390"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1784..1786,aa:Thr,seq:cgt)
     gene            1828..1901
                     /locus_tag="VFB89_12395"
     tRNA            1828..1901
                     /locus_tag="VFB89_12395"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1860..1862,aa:Glu,seq:ttc)
     gene            2036..2110
                     /locus_tag="VFB89_12400"
     tRNA            2036..2110
                     /locus_tag="VFB89_12400"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2069..2071,aa:Arg,seq:acg)
     gene            2122..2210
                     /locus_tag="VFB89_12405"
     tRNA            2122..2210
                     /locus_tag="VFB89_12405"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2157..2159,aa:Ser,seq:gct)
     gene            2216..2305
                     /locus_tag="VFB89_12410"
     tRNA            2216..2305
                     /locus_tag="VFB89_12410"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2249..2251,aa:Ser,seq:cga)
     gene            2398..>3272
                     /locus_tag="VFB89_12415"
     CDS             2398..>3272
                     /locus_tag="VFB89_12415"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZM28143.1"
                     /translation="MSDVGQAFQVLPLENGLVELRFDLPGESVNKFNAVALASLKTAV
                     EGLRSRKGLRGVLLTSGKEVFCVGADITEFLTHFKRSEAELTAWVLETDAVFSALEDL
                     EAPSVVAINGICVGGGFELSLTASYRAMSQAAQVGLPESKLGLFPGWGGTVRLSRLCG
                     ADTAIEWIAGGEQWSAADALKAGAVDAVTAPADLREAALGMLQEAADGKLDWKARRAE
                     KAAPLKLNAIEAGMVFASSRAFVAGKAGPHYPAPLAAIEVIEKGGGKSRDEALAIEAA
                     GFAKIAKTSAAHALV"
BASE COUNT          590 a         1009 c         1088 g          585 t
ORIGIN      
        1 accggcctcg tcaacatcat cgtgaagccc gaccagttcg agcggtggaa ggacgtcctc
       61 gtgacctcgc cggtcctcga gatccacggc ttcctgcagt cccaggaagg gatggcggtg
      121 cgggcgctaa gggcccggcc agtggaaacg gcgcaggctg tcagccgctc gcgggacttc
      181 cactgaattt cgttcggaat gcaatttcgc tattgacaag tcggaagggg gctcgtatat
      241 tcgcacgcga ggttgatgtg actattcgtt tcttcaattc gccgggccca tcgctagcgc
      301 ggccatctta tttggccggc gtcgagcgag ccgccatgtg tgtgcgcggt agttcgcttg
      361 ataacccgaa gggggacaaa gcgccctaaa ggtcctgcat tcatagagaa ccaggccttc
      421 tagggtgatc ccctagaagg ccttttttat tgggcttcga gcccgagaga cacgggcacg
      481 agggcatagg gaggttctgt cttcgaaaac ggtgaagtcg tgacgccggc agcgtggtgt
      541 aacggaagca tcggagtctc caacactcgc gggtccaggt tcgaatcctg gcgctgtcgc
      601 caacacgggc ggtacgctcg caagagcggg gtctttgaca agtgacggac gtcaatgggc
      661 cggtgagcga caccggtcca tgttcggcct gggtgtgcag ggagcatccc gcgttgccag
      721 cgcggaggga cgggttcgat tcccgtaggc cgaaccaaag cgagtgtggg gtaacggtcg
      781 cctgcaggcc ttccaagcct tgaggtgcgg gttcgaatcc cgccattcgc tccaacgctg
      841 atctgatgta acggaagcct gcggccgtgg tatggccgat gtccgggttc gatccccggg
      901 atcagctcca ctcccctctg tagtgtcaac gcagcatgcc gggctcttaa cccgtgtggt
      961 ggtggttcga gtccacccgg agggaccatc tcggctcgtg gcgcaatcgg cagccgcggc
     1021 acgctcagac cgtgcagatc ggggttacgg gtcgactccg cttcgcgcca gtcgtcccgt
     1081 ttcgcctcgc tggcgctcgg ggtccgaatc gccgcgagcc gaccatcctg agggggacat
     1141 agtcgaaggg aaagacgccg cgttgtcagc gcgggagtac gggttcgagt cccgttgtcc
     1201 tcgccaggat gggcttgtcg ttcaacggga ggatccctgg ctcgcatcca ggagatgagg
     1261 gttcgattcc ctccttgtcc accaacgggt tcttgctgcg gagggaaggg acggtgacct
     1321 tggaggtctc ataagcctcg gcatgcgggt tcgattcccg cctccgctac cagaagccgg
     1381 ttcgttcaac ggaaggacgg cggttttaca agccgctcat gggggttcga ttccctcacc
     1441 ggctaccagt ttcgaacggt gggcgtaaga taacggccag tcggggaggc tgtgtccctc
     1501 ccagcgccgg ttcgattccg gccgctcacc ccaatctcac acctcgggtc gtagggtaat
     1561 cggtagccct cctgcctcgg acgcaggcac gtggaggttc gagtcctctc gacccgacca
     1621 ttgacagatc gtccaacggc aggacggcgg gttctgaacc cgcagatctc agagcagcga
     1681 gctagtgtgg tcatagcgct cggccgaagc ccgagagaac gtggttcgat cccacggctg
     1741 gtcaccatga tcacgccacc aacgctcagg agcgtagctg tcccgtaagc agcgagaggg
     1801 agttcaactc tccctggtgg ctccaacggg ggcatgcggt aatggcagcc cggcagcttt
     1861 tcacactgtc agtctgggtt cgagtcccag tgcccctacc acgtcgcctg gtgagaggga
     1921 gatcacacga gcctccgaag ctcgaagtcg cggttgagcg tcgccttcgc caaggcgaag
     1981 ccgacactct tctactcgct gacgctcgca gtccgactcc gcgggcgacg accatcccct
     2041 cgtaggctaa tgcacagacc gccgggctac gaacccgtca atggaggttg gagtcctccc
     2101 gaggggacca ccgcaatatc gggagaggaa agggcgagcg gggagcccag cacactgcta
     2161 atgtgccgag gcctgaggcc acgagagttc gaatctctcc ctctccgcca tgtccggagg
     2221 gctagcccaa ttggtaaggc acccggtccg aacccgggca aggcgcgcga gcgccatgag
     2281 agttcgagtc tctcgccctc ctccattcgt gaagaccgcc ggcggcaaac ggccgacgcg
     2341 caacgcgcgc tagaatggcc gaggtcgcag tccgcgacct tcttctagga gcaacccatg
     2401 agcgacgtag ggcaggcctt tcaggtcctg ccattggaga acgggcttgt cgagctgcgc
     2461 ttcgacttgc ccggcgagtc cgtgaacaag ttcaacgctg tggcgctcgc ctcgctgaaa
     2521 acggcagtcg agggcctgcg ctcgcgaaaa ggcctgcgcg gtgtgctgct gacgagcggc
     2581 aaagaggtct tctgtgtggg cgccgacatc acggagttcc tgacccactt caagaggagc
     2641 gaggccgagc tcaccgcctg ggtcctcgag accgacgcgg tcttctcggc tctcgaagac
     2701 ctggaagcgc cctccgttgt cgcgatcaac ggaatctgtg tgggcggcgg cttcgagctc
     2761 agtctcactg ccagctaccg cgccatgtca caggccgcgc aagtgggcct gcccgagtcg
     2821 aagctcgggc tcttccccgg ctggggcggg accgtgcggc tctcacggct atgcggagcc
     2881 gatactgcga tcgagtggat cgcaggaggc gagcaatggt cggcggcgga tgcgctcaaa
     2941 gccggcgcgg tcgacgcggt cacggcaccc gctgacctgc gcgaggcggc cctcggcatg
     3001 ctgcaagaag cggcggacgg gaagctcgac tggaaggccc gacgcgcgga gaaggcggct
     3061 ccgctgaagc tcaacgcgat cgaggcgggc atggtcttcg cctcctcgag agcgttcgta
     3121 gcgggcaagg ccggcccgca ctaccccgct ccgctcgccg cgatcgaggt gatcgagaag
     3181 ggcggcggca agagccggga cgaggcgctg gcgatcgagg ctgcgggctt cgcgaagatt
     3241 gcgaagacga gcgccgccca cgccctcgtc ag
//
LOCUS       DASRJQ010000541         2174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_637877, whole genome shotgun sequence.
ACCESSION   DASRJQ010000541 DASRJQ010000000
VERSION     DASRJQ010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637877"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2174)
                     /gene="dnaE"
                     /locus_tag="VFB89_12420"
     CDS             complement(<1..>2174)
                     /gene="dnaE"
                     /locus_tag="VFB89_12420"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HZM28144.1"
                     /translation="KIVTEGMLRLGRELGIGVVATNDAHYLRREDAEAHDVLLAIGTG
                     ADLDDPKRFRFLGQESYVKSEKEMRALFRDHPEALDNTARVADLCEFSFEKRYYLPGF
                     PRPAEYASDEALLEHLATHGAEARYGAALPEAVKERLDYELGVINRAGYAGYFLIVQD
                     FIAAARNRGIPVGPGRGSAAGSLVAYSLGITNVDPLRFDLLFERFLNPERVSMPDIDV
                     DFCFERRGEVIEYVRERYGRDSVGQIVTFGTMKARAAVKDVARVLRIPPGDADRLTKL
                     IPSGPAYSLTIPEAADRITEIKDLMRSDATYQRLIDLSSRIEGISRHMSVHAAGVVIA
                     PGPLAEYVPVCTAPTKGAGQAADGDDAIITQYDMVALEKVGMLKMDMLGLKTLTVIQD
                     ACALIHERTGERLDMDAVPFDDPAAYALLRAGRTAGIFQFESPLATDTLRAIKCDRFD
                     DLVACNALLRPGPLDAGMHTVFIRRKLGLERVTYLHPSLKEILEPTYGVITYQEQVMR
                     IANVLAGFSLAEADVLRKAVGKKDQELANQEVAKFVERGVAQGHPRHVIEELGRQIVT
                     FGRYGFNKSHSVAYSVLSYQTAWLKAHHPAEFMAALLSSEIGNTDKVVQYINEARELG
                     LEVLPPDVNESGWKFTVVGPERMRFGLGAIRNVGRGAIESILDARGAGPFRSLSDLVD
                     RIDLRICNKRVLESLVAAGACDSLGGHRKQQAEALDRVLAEA"
BASE COUNT          378 a          709 c          699 g          388 t
ORIGIN      
        1 gtgcctcggc gagcacccga tcgagcgcct ccgcctgctg cttgcggtgg ccgcccaggg
       61 agtcgcaggc gccggcggcg accagggact cgagcacccg cttgttgcaa atcctcaggt
      121 cgatgcgatc taccaggtcg ctcaacgatc tgaacggccc ggcgccgcga gcgtcgagga
      181 tcgattcaat ggcgccgcgc cccacgttgc ggatcgcgcc cagcccgaat cgcatccgct
      241 ccggccccac cacggtgaac ttccaacctg attcgttcac gtccggcggg agcacctcga
      301 gtcccagctc ccgggcctcg ttgatgtatt gaacgacctt gtcggtgttc ccgatctccg
      361 aagacagcaa cgcagccatg aactcggcgg gatggtgtgc cttgagccag gccgtctgat
      421 aggagaggac cgagtaggcg accgaatgcg acttgttgaa gccgtaccgt ccgaaggtga
      481 cgatctgccg gcccagctcc tcgatcacgt ggcgtggatg cccctgggcg acgccgcgtt
      541 ccacgaactt ggccacctcc tgattggcca gctcctggtc cttcttgccc acggccttcc
      601 gcagcacgtc ggcttcggca aggctgaatc cggccagcac gttcgcgatc cgcatcacct
      661 gttcctgata ggtgatgaca ccgtaggtgg gctcgaggat ttccttgagc gacggatgga
      721 ggtaggtgac ccgctccagt ccgagcttac gccggatgaa gacggtgtgc atgcccgcat
      781 ccagcggacc ggggcgcagc agcgcattgc aggcgaccag atcgtcgaac cggtcgcact
      841 tgatggcacg caaggtgtcg gttgcgagcg gtgactcgaa ctggaaaatg ccggccgtac
      901 gaccggctct gagcaacgca tacgccgcgg ggtcatcgaa cggtacagcg tccatgtcca
      961 ggcgctcacc ggtgcgctcg tgaatcaagg cgcaggcatc ctggatgacc gtcagggtct
     1021 tgaggccgag catgtccatc ttgagcatgc cgaccttctc gagtgccacc atgtcgtact
     1081 gggtgatgat ggcgtcatcg ccatcggcgg cctggccggc gcccttcgtc ggggccgtgc
     1141 acaccggcac atactcggcc agcggacctg gggcaatcac gacgccggcg gcatggaccg
     1201 acatgtgtcg ggagatgcct tcgatgcgcg agctgagatc gatcagacgc tgataggtcg
     1261 cgtcgctgcg catcagatcc ttgatttcgg taatccgatc cgcggcttcc ggaatcgtga
     1321 gactgtacgc cgggcccgac gggatgagct tggtgagccg gtcggcgtcg ccgggtggga
     1381 tccggagcac gcgggccaca tccttgacgg ccgcgcgcgc tttcatcgtt ccgaaggtca
     1441 cgatctggcc gacgctgtcc cgaccgtagc gctcccgcac atactcgatc acctcgcccc
     1501 gccgctcgaa gcagaagtcc acatcgatgt ccggcatcga cacccgctcg ggattgagga
     1561 agcgctcgaa caggagatcg aatcggagcg gatcgacgtt ggtgatcccg aggctgtagg
     1621 ccaccagtga tcctgcggcc gagccgcggc ccgggccgac cggaatgcca cgattgcgcg
     1681 ccgcggcgat gaagtcctga acgatgagga agtagcccgc gtatccggca cggttgatga
     1741 cacccagctc gtaatcgagc cgttccttca ccgcctccgg cagcgccgcg ccgtagcggg
     1801 cttcggcgcc gtgagtggcg aggtgctcca gcaaggcctc gtcgctcgca tactcggcgg
     1861 ggcggggaaa gcccgggagg tagtatcgct tctcgaatga gaactcgcag aggtcagcca
     1921 cccgagccgt attgtccagc gcttccgggt ggtcccggaa cagcgcccgc atttcctttt
     1981 ctgacttgac gtacgattcc tgtcccagga agcggaaccg cttcgggtcg tcgagatcag
     2041 ctccggtacc gatggcaagc agaacgtcat gcgcctcggc gtcctcgcgc cgcagatagt
     2101 gagcatcgtt ggttgcgacg acgccgatgc ccagctcgcg gccgagccgc agcatccctt
     2161 cggtgacgat cttt
//
LOCUS       DASRJQ010000542         2763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3627088, whole genome shotgun sequence.
ACCESSION   DASRJQ010000542 DASRJQ010000000
VERSION     DASRJQ010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3627088"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1566)
                     /locus_tag="VFB89_12425"
     CDS             complement(<1..1566)
                     /locus_tag="VFB89_12425"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZM28145.1"
                     /translation="MASSLDNNVGLPPEADLPSLLERLRSALAPEYGVDREIAAGGMA
                     HVFLARDVRLDRLVAVKVLKPELATAIGAERFLREARVHAQLKHPNVVPIHVADERDG
                     LSFYVMDYIDGETLAQRLTRGALAPDEVRSLGRELLAALAAVHALGITHRDIKPGNVF
                     LTPRVLLTDFGIARSKEDETLTQEGQILGTPHYMPWEQRVGDATPATDVCATAALLFE
                     AATGRRWNFSALDQPADWSGVPRSLQRALVPGLAFNPVDRPSASRLQAALAGRSLNRS
                     TVFYGAAALALILIAIAFRRPRPLPPPPVDFAILPFTALGDTSTIGHEVAQYAARPLE
                     WFPAWSIRPTPATFAWWESASPSDRLTRAASTLRARLVVDGDLVQRSSESSFHLTIRD
                     STGALVALSDVPGSPSDLITWGNAVADSIVRLTYHEQLADYRRAAGGPSINAAAYREF
                     FRGQDAFRQDNWVAAEAHLRRALEHDTTFSHAAWQLALLLVWRRDSSAIPVLQKLYAT
                     GQTDLPELQRLLTE"
     gene            complement(1615..>2763)
                     /locus_tag="VFB89_12430"
     CDS             complement(1615..>2763)
                     /locus_tag="VFB89_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28146.1"
                     /translation="RVWEAATRLQPYFTAYVHMAVGQIRLGNRAAASQALDALRHDRD
                     ATNAEAQQRASLLGLVFDQRFRPWRAWIALSFLDWRADSAAAVRLSQYTRLGLVFDVP
                     EAQRRVGRILVRRSRDPAVQANGHEAQGLALMAQGRPMQAIVQFDSAAALFGTPDAEL
                     ERAEWRLLPAALGLPASDTAARKWARHTLAAAADGPGAARAAWALAVDAEASGDSEDF
                     TRWRDRLIALPPDSSGSHRLGRLLDALRRAGAGDRAGALAITDSLLTYDGGSLTLAPF
                     ARALLYLRRGEWLAAEGMPERADRAWEWYEGWDIDGWAQRGAQSGEVDAVASVIARLR
                     RAELAARRGDLATACDFTTRVRELWSQAEPSYQALLGRARTVGAACRG"
BASE COUNT          505 a          897 c          923 g          438 t
ORIGIN      
        1 ttcggtgagc aggcgctgaa gctccgggag gtcggtctgt ccggtcgcat acagcttctg
       61 caggacgggg atggcggagc tatcacgccg ccaaaccagg agcaaagcca actgccatgc
      121 tgcgtgcgaa aacgtcgtat cgtgttcgag tgcccgtctc aggtgggctt ccgcggccac
      181 ccagttgtcc tgacgaaatg cgtcctggcc acggaagaat tcccggtacg ctgcggcgtt
      241 gatgctgggt cccccggccg cacggcgata atcagccaac tgctcgtgat acgtcaggcg
      301 gacaatggag tccgccacag cgttgcccca ggtgatcagg tcgctgggcg atcccggcac
      361 atcagaaagc gcgaccaacg cgccagtcga atcgcggatg gtaagatgga atgaggactc
      421 cgaagaccgt tgcaccaggt cgccgtccac gaccagccga gcacggagcg tcgaggcggc
      481 tcgagtcagc cgatcactcg ggctggcaga ttcccaccat gcgaacgttg caggagtcgg
      541 gcggatgctc caggctggaa accattccag cgggcgcgcg gcatattggg cgacttcatg
      601 gccgattgtg gatgtgtccc cgagagccgt gaaggggaga atggcgaaat cgaccggcgg
      661 tggtggtagg gggcgcggac ggcgaaatgc aatcgctatc agaatcagag cgagagccgc
      721 cgccccgtag aacacggtgc tcctgttcaa cgaacgcccc gcgagtgccg cctggagacg
      781 gctggcggac ggccgatcaa ccgggttgaa agccaatccg gggacaagtg ctcgctgcaa
      841 actgcgggga actcccgacc aatcggcagg ctgatcgagc gccgagaagt tccaacgacg
      901 gccggtagcc gcctcaaaaa gcagagccgc tgtagcacag acgtcggtag ccggcgtggc
      961 gtctccgacc cgttgctccc agggcatgta gtggggcgtc cccaggatct gcccttcctg
     1021 agtcagcgtc tcgtcttctt tcgaacgcgc aatgccgaaa tccgtcaaca agacccgtgg
     1081 ggtcaggaac acattgccag gcttgatgtc gcggtgggta attcctagtg catgcacggc
     1141 ggccagtgca gccagcagct ctcgacccaa tgaccgcacc tcgtccggtg cgagcgcgcc
     1201 gcgggtgagc cgctgcgcca gcgtttcgcc gtcgatgtaa tccatgacgt agaacgaaag
     1261 gccgtcccgc tcgtcagcga cgtggatggg gaccacgttg ggatgcttga gttgggcatg
     1321 gacgcgcgct tcccgcagga atcgttctgc gccgatcgca gttgccagct cgggtttgag
     1381 gaccttgacg gcgaccagcc ggtccagccg aacgtctcgt gccaggaaga cgtgggccat
     1441 gccgccggcg gcgatctcgc gatcgacgcc gtattcgggg gcgagagcgc tacgcaaacg
     1501 ctccagcagg gagggcaggt cagcctccgg aggaagtccg acgttgttgt cgagagagct
     1561 agccaagata gggggcgcgc gggccttggc aactctgcag cgtgggtact ggcgtcagcc
     1621 tcggcaggcc gccccgaccg tgcgggcccg acccaacaac gcctggtatg agggttccgc
     1681 ctgcgaccac agctcgcgta ctcgagtggt gaaatcgcag gcggttgcga gatcgccccg
     1741 tcgcgcagcg agctcggcgc ggcggagccg tgcgatcacg ctggcgacgg catcgacctc
     1801 gcccgactgg gcgccgcgtt gggcccagcc gtcgatatcc cagccttcat accactccca
     1861 cgcgcggtct gctcgttcgg gcatcccttc agcggcgagc cattccccac gacgcaggta
     1921 gaggagcgcg cgggcgaaag gcgctagagt cagcgaccct ccgtcataag tgagcaaaga
     1981 gtcggtgatg gccagagcgc ctgcccgatc accggcgccc gcccggcgca gggcgtcgag
     2041 caatcgtccc aatcggtggc tgccggagct gtcgggcggg agggcaatga gccgatcccg
     2101 ccagcgggta aagtcctcgg agtcgcctga ggcctcggcg tccaccgcca gggcccacgc
     2161 agcacgagcg gcgccgggcc catccgctgc cgcggccagc gtatggcgcg cccatttcct
     2221 cgccgccgtg tccgatgcag gaagccccag tgccgccggc agcagtcgcc attctgcccg
     2281 ctccagctcg gcgtcaggtg ttccgaacaa tgccgcagcg gagtcgaatt ggactatcgc
     2341 ctgcatgggg cggccttgag ccatcagcgc gagcccttga gcctcgtggc cattcgcctg
     2401 tacggccgga tcgcggctcc gtcgcaccaa aatccggccc acccggcgct gtgcctccgg
     2461 aacgtcgaac acgaggccga gtcgggtgta ctgtgacagt cgcacggcag cagccgaatc
     2521 tgcgcgccaa tcgaggaagc tcaacgcgat ccacgcgcgc cagggtcgga atcgttggtc
     2581 gaagaccaat cccagaagtg acgcacgctg ttgcgcctcg gcattcgtcg cgtctcggtc
     2641 atgcctcagc gcatcgagcg cctgacttgc cgcagccctg ttcccgagcc gaatctggcc
     2701 caccgccatg tgaacgtagg ctgtgaaata gggttgcagg cgtgtcgccg cctcccagac
     2761 ccg
//
LOCUS       DASRJQ010000543         4938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2376035, whole genome shotgun sequence.
ACCESSION   DASRJQ010000543 DASRJQ010000000
VERSION     DASRJQ010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2376035"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VFB89_12435"
     CDS             <1..745
                     /locus_tag="VFB89_12435"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HZM28147.1"
                     /translation="NNQRVGQARAQLSWSRAQEASARATVASAEAGFKKAAADLERIR
                     GLAANKIVPAQQLDAAQAEYDAAVAGLESARKQAAAGADQVVMYGAALSGAGARLEAA
                     QAAVDNARLQLSYTYILAPTDGVIARRSAEVGALVQIGQTLMSLVPDRGLWVTANLKE
                     TQLDNVRVGDSATFTVDAYGGHVFHGHVVSLSPATGARFALLPPDNATGNYTKVVQRV
                     PVRIAVDDAPDQAYPLRPGMSVVPTITTR"
     gene            751..2340
                     /locus_tag="VFB89_12440"
     CDS             751..2340
                     /locus_tag="VFB89_12440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZM28148.1"
                     /translation="MATLALPSRSGGLAQKDVYRYRYIIAFAVALASVLELVDTSIVN
                     VAIPHMMGNLGATLDEIAWVSTGYIVANVIILPLTSWLSERFGRRNYYTGSIVLFTVA
                     SFFCGNAHSLEVLVGGRVVQGIGGGALISTAQAILFDVFPIEERATGQAIFGMSVMVG
                     PTLGPTLGGWITDNYTWPWIFYINIPLGILAGFLSWRFVPEPSHASGQKTEKVDWWGL
                     AFLIIGIGALQIMLERGERRDWFETREIVVEGLVALTGIVCFIWHELRTAHPMVDLRV
                     LKNRQLAVGVTFGALLGFALYSSVFALPVFLQNLLGYTAWDTGKVILPGAIASAIAMA
                     SMGRLVPKVDARVLIVTGVSLFFCSMWMHYHFTLDTGHHELFWAMVLRGFGIGLVFVP
                     LTAATVADLKPGQIPQGTGLFNLARQLGGSLGIAVTATMLAHFTEQARGALVAHVTNY
                     DAATQSWLSSVTASMQRFGGTVAEAQLRASALLDFKLGQQASIFSFERVFLIMGAAFA
                     SALPLLLLFRTGRARGGGMAH"
     gene            2372..3856
                     /locus_tag="VFB89_12445"
     CDS             2372..3856
                     /locus_tag="VFB89_12445"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZM28149.1"
                     /translation="MTFQRKLLLGFSLMALPALLVGVEAIRSNALERHALETLGASLS
                     RTRTYADVENAMFDQTEAIWRQLSGLDPDARKEWRLSGEVVDYWLDRWSAELRPDEVE
                     LANAVREVQRQIRAVGDSVIALSDAGHREEGYRIARQEIKMRLLPTLANLNREIYRKA
                     REFSVQQAFAKVEAIVNTERQILFGIVLLALGCGLAGAFLITQSLVRPITQLREAMAV
                     VGAGDLDHPIAVRRRDEIGDLARAFADMTEQLRRTQAKLVQSEKLASIGEMSAAVAHG
                     LRNPLASLRAAAQFVLKRPESPASREQLSAIIDEVDRLDRRITHLLSFSRPAPFHPTR
                     ESVTTLVEGILPAFTQLLEQRKVELKLDLPADLPDIRVDPLQLEQTLVEIVSNALDAM
                     PDGGRLAISGRREPMAAGGSDGIVLEISDTGDGIPEHALPQITQPFFTTRPEGTGLGL
                     AIAKRYIEQNGGRLELTSRVREGTTVGVHLPVAEPGGVAAGSGA"
     gene            3853..>4938
                     /locus_tag="VFB89_12450"
     CDS             3853..>4938
                     /locus_tag="VFB89_12450"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZM28150.1"
                     /translation="MSGPSVLIVDDERTLARAVRTFLAESGYEAEVAVDAESALGMLE
                     QLRPDVVFTDVRLPGMSGIDLLRRIREFDPAVPVIIMTAHGTIEGAVEAVKLGAFDYL
                     KKPVDLEELKLLADRAREQSRREGELSYYRRQARETPFGGVVGDSPAMRAVLERARQI
                     AALDETPPVLITGETGTGKGLVARTIHSSGSRGSRPFIEVNCTALPGSLMEAELFGYE
                     RGAFTDAKESKVGLFEAAEGGFIFLDEVGDIQLALQGKLLRAIEERTVRRVGGIRDRK
                     IDVRILAATNRDLERESRREAFRKDLYFRLAVILLRLPSLRERGDDVLRLAEHYLRQF
                     SVKYGKDVRRIEPAAQDRLLAYRWPGNV"
BASE COUNT          845 a         1583 c         1626 g          884 t
ORIGIN      
        1 caacaatcag cgggtcggcc aggctcgagc ccagctctcc tggagccggg cgcaggaggc
       61 cagcgcgcgg gctacggtgg cgtcggctga ggccggcttc aagaaggcgg cggctgatct
      121 cgagcgcatt cggggtctgg ccgccaacaa gatcgttcca gcccagcagc tggacgcggc
      181 acaggcggaa tacgacgccg cagtggcggg actcgaatcc gctcggaaac aggcggcggc
      241 cggcgcggat caggtcgtca tgtatggcgc cgccctctcc ggtgcgggcg cccggctcga
      301 ggcggcgcag gccgcggtag acaacgcgcg cctgcagctc agctacacct acatccttgc
      361 gcccacggac ggcgtcatcg ccaggcggag tgccgaggtc ggtgcgttgg tgcagatcgg
      421 acagacgctg atgtccctgg tacctgatag ggggctctgg gtcactgcga atctcaagga
      481 aacccagctc gacaacgtgc gcgtgggcga ctcggcgacg tttacggtgg acgcctacgg
      541 cggacacgtt ttccacggcc atgtggtgag cctgagccca gccaccggag cgcggttcgc
      601 cctgctgcca ccggacaatg cgaccggcaa ctataccaag gtggtgcagc gagtcccggt
      661 gcgcatcgca gtagatgacg ctccggatca agcctaccct ctgcgacccg gcatgtccgt
      721 agtgccgacc attacgacgc ggtaacccac gtggccacgc tcgcactgcc ctcgcgcagc
      781 ggcggactcg cccaaaagga cgtttaccgc taccgctata tcatcgcttt cgcggtcgcg
      841 ctggcgagcg tcctcgagct ggtcgacacc agcatcgtca acgtcgccat tccgcacatg
      901 atggggaacc tgggcgccac gctggacgag atcgcgtggg tgtcgaccgg ctacatcgtc
      961 gccaatgtga tcatcctccc cctcaccagc tggctgtcag aacggttcgg acgacggaac
     1021 tattacaccg gctcgatcgt gctgtttacg gtcgcgtcgt tcttttgcgg caacgcgcat
     1081 tcgctcgagg tgctggtcgg aggccgggtg gttcagggca tcggtggcgg cgcgctgatc
     1141 tcgacggcgc aggccattct gttcgacgtc ttccctatcg aggaacgcgc caccggtcag
     1201 gcgattttcg gcatgagtgt gatggtcggc cccaccctcg gccccaccct cggcggctgg
     1261 atcaccgaca actacacctg gccctggatc ttctacatca acatcccgct cggtattctg
     1321 gcgggcttcc tgagctggcg cttcgtcccc gagccctcgc atgcatccgg gcagaagacg
     1381 gagaaggtgg actggtgggg cctggcattt cttatcattg gcatcggagc gctccagatc
     1441 atgctggagc ggggcgagcg cagggactgg ttcgagacac gcgagatcgt ggtcgagggg
     1501 ctggtcgcac tcacgggcat cgtctgcttc atctggcacg agctgcgcac cgcgcatccg
     1561 atggtggatc tgcgcgtact caagaaccgg cagctggcgg tcggcgtgac gttcggcgca
     1621 ttgcttggct tcgcactcta ctccagtgtg tttgccttgc cggtgttcct ccagaacctg
     1681 ctcggctaca ccgcctggga cacgggcaag gtcattctgc cgggcgcgat cgccagcgcc
     1741 atcgctatgg cttccatggg ccggctggtg ccgaaagtcg acgcgcgcgt gctcattgtc
     1801 accggcgtgt cgctgttctt ctgctccatg tggatgcact atcacttcac cctggatacc
     1861 ggacatcacg agcttttctg ggcgatggtg ctccgtggct tcggcatcgg gctggtcttc
     1921 gtgccgctca ccgcggcgac cgtcgcggac ctcaagccgg ggcagattcc ccagggcacg
     1981 ggcctgttca acctggcccg ccagctgggc gggagcctcg gtatcgcggt gacggcgacc
     2041 atgctcgcgc atttcaccga gcaggcgagg ggggccctgg tggctcacgt caccaactac
     2101 gacgccgcga cccagagctg gcttagcagc gtgaccgcct cgatgcagcg attcggcggc
     2161 acagtagccg aggcccagct gcgtgcatcg gcgctgctcg acttcaagct cgggcagcag
     2221 gcatccattt tctcgttcga gcgggtcttt ctgatcatgg gcgcagcctt cgcgtccgcc
     2281 cttccgctgt tgctcctgtt ccgcacgggg cgggcccggg gaggcgggat ggcgcactga
     2341 cgcgacggct ttcatacttc tcgccgctcc catgaccttc caacgcaagc tcctgctcgg
     2401 tttctctctg atggccttgc cagccctgct ggtcggcgtc gaggcgattc gcagcaacgc
     2461 gctcgagcgc cacgccctgg aaacgttggg tgcgagcctg tcccgcaccc gcacatatgc
     2521 cgacgtcgaa aatgcgatgt tcgaccagac cgaggcgatt tggcgtcagt tgagcggcct
     2581 cgaccccgat gccagaaaag agtggcgtct aagcggcgag gtggtggatt actggctcga
     2641 tcgctggagc gcggaactcc gcccggacga agtcgagctg gctaacgccg tgagagaggt
     2701 acagcgccag atccgagccg ttggagacag tgtgatcgcc ttgtccgacg cggggcatcg
     2761 cgaagagggt taccggatcg cgcggcagga aatcaagatg cgactgctcc ccaccctggc
     2821 aaacctgaat cgcgagatct accgcaaggc gcgcgaattc agcgtccagc aggcgttcgc
     2881 gaaagtagag gccatcgtca acacggagcg ccagatcctg ttcggcatcg tgctgctggc
     2941 actcgggtgc ggcctcgccg gagcctttct catcacccag agtctggtac gaccgatcac
     3001 tcagctgcgt gaagccatgg ccgtagtcgg cgcgggagat ctcgaccatc cgatcgcggt
     3061 gcggaggcga gacgagatcg gggacctggc tcgcgcgttc gccgacatga ccgagcagct
     3121 tcgtcgcacg caggccaagt tggttcaatc ggagaagctc gcctcgatcg gcgagatgtc
     3181 cgccgccgtc gcccacggcc ttcggaaccc gctggccagt ctccgtgctg ccgcgcagtt
     3241 cgtgctcaaa cgccccgaat caccggcttc acgagagcag ttgagcgcca tcatcgatga
     3301 agtcgatcga ctggaccggc gaatcacgca cctgctcagt ttttcgcgcc ccgccccttt
     3361 ccacccgacg cgggaaagcg tcacgacgct ggtcgagggg atattgccgg ccttcaccca
     3421 gctgctggaa cagaggaagg tggaattgaa actcgatctc ccggccgacc tgccggacat
     3481 acgggtcgat ccgctgcaac tcgagcagac actggtagag atcgtctcca atgcactcga
     3541 cgccatgccc gatggcggcc gactggccat ctccggccgg cgcgagccaa tggctgcagg
     3601 cggatccgac gggatcgtgc tcgaaatctc cgacacgggc gacggaattc cggagcacgc
     3661 gttgccgcag atcactcaac cattcttcac cactcggccc gaaggcaccg gactcgggct
     3721 cgccatcgcg aagcgctaca tcgagcagaa cggcggtcgg ctggagctca catcccgggt
     3781 gcgagagggg acgaccgtag gagtccacct gccggttgcc gaacccgggg gcgtggcagc
     3841 ggggagcggc gcgtgagcgg accaagcgtt ctcatcgtgg atgacgagcg gacgctcgcg
     3901 cgcgcggttc ggaccttcct cgcggagtcg gggtatgaag ccgaggtcgc ggtcgatgcc
     3961 gagagtgctc tcggcatgct cgagcaactc aggccggacg tggtctttac cgacgtgcgc
     4021 ctgcccggga tgagcgggat tgacctgctg cgccggatcc gcgaattcga cccggcggtt
     4081 ccggtgatca tcatgaccgc ccacggcacg atcgaagggg cggtcgaggc ggtgaagctg
     4141 ggcgccttcg actacctgaa gaagccggtt gatctcgagg agctgaagct cctggccgac
     4201 cgcgcccggg agcaatcccg tcgcgaaggt gagctgtcct actatcgcag gcaggcacgg
     4261 gagacccctt ttggcggcgt ggtgggcgat tcaccggcca tgcgcgcggt gctcgagcgt
     4321 gcccggcaga tcgccgcact cgacgagacc cctccggtcc tgattacggg tgagaccggt
     4381 accgggaagg gcttggtcgc caggaccatc cactcgtccg gctcccgcgg ctcacgtccg
     4441 tttatcgaag tgaattgcac cgcgctaccc ggcagtctca tggaggccga gctgttcggc
     4501 tacgagcgtg gcgcgttcac ggatgcaaaa gaatccaagg tcggcttgtt cgaagcggcg
     4561 gaaggcggat tcatcttcct cgacgaggta ggtgacatcc agctggcgct ccagggcaag
     4621 ctgcttcgcg caatcgaaga gcgcaccgtg cgccgggtcg ggggtattcg agatcgtaag
     4681 atcgacgtgc gaatcctcgc ggccacgaat cgcgatctcg agcgggaatc ccggcgggag
     4741 gcattcagaa aggatctcta ttttcgactg gccgtcatcc tgcttcgact tccttcgctt
     4801 cgcgagcgcg gcgatgatgt cctgcgactg gccgagcact atctcagaca gttcagcgtg
     4861 aagtacggga aggacgtgcg ccggatcgag ccggccgcgc aggatcgctt gctggcgtat
     4921 cgctggccgg ggaatgtg
//
LOCUS       DASRJQ010000544         5805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2355431, whole genome shotgun sequence.
ACCESSION   DASRJQ010000544 DASRJQ010000000
VERSION     DASRJQ010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2355431"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1637)
                     /locus_tag="VFB89_12455"
     CDS             complement(<1..1637)
                     /locus_tag="VFB89_12455"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HZM28151.1"
                     /translation="MTRRPVASWLPWAVFALGVAISIGAAWAAATRARADAKVAFDHR
                     GDLAIATLSDRLRLCIQAARAATGLFAASEEVTRSEWRDYVHHAGADTAFPGLVGLEF
                     IERVPAAELVAHVRRVQSEGLQDYQVWPAGGTGDAYPILYAESLRDAPAERLGFDIAT
                     DPVRRQALERARDSGMPVVSGRLTLTSDRTPTPSLVVLAPVYPEGVVPPTVAGRRAAM
                     VGLVAAAVHAPSLVAGMLGAVPGLDVEVFDGPTPRADRILYAADAVVVATGAGRQPAL
                     TRTERMNAAGRPWTIFLSTMPDFEQGIVARGSVAILLGGIALSLAVSLALRAQLLVGE
                     RAEVLATRMTAELRKTEANLELDVAVRQRVEAALRESEERYRSLLENTAVGIYVDVGG
                     RFAYANRELVRILGASAASGILGREILPHIAPPVRAIVAERMTSGRGEATPLKELQLI
                     NLQGRLVDVEASALPVVFDGQLATQVQVRDISDRKEAERGRASLEIQLHEAQKMEAIG
                     TLAGGIAHDFNNILGAIVGYTELAMADAADPSAVRENL"
     gene            complement(1634..2347)
                     /locus_tag="VFB89_12460"
     CDS             complement(1634..2347)
                     /locus_tag="VFB89_12460"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HZM28152.1"
                     /translation="MQTVHRYERLPSTQDEAHRLAAEGAPAGTVVVAAEQVEGRGTRG
                     RNWTSPVGGLWLSFLCRPTRLPGVEVLSLRVGLAAAEALAEVGGLPPASLKWPNDVIV
                     NDRKVGGILCEARWHGDLLAWVVVGLGLNVRNPLPTDARVAPGRLADWRQDLEPDHIL
                     WPLVGSLLPLAETGSGLSAAELRAFGQRDWLLGRELAEPERGRAAGIEPDGALRVLKP
                     DGQIVRVSSGSVVLARDAP"
     gene            complement(2356..3183)
                     /locus_tag="VFB89_12465"
     CDS             complement(2356..3183)
                     /locus_tag="VFB89_12465"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF001390.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HZM28153.1"
                     /translation="MDLSPLAQLHPLVKAAILGVVEGATEFIPVSSTGHLIVAGDWLA
                     FTGESAKAFEIFIQLGAILAIVWLYRSDVARAIETHKSGILRHQLVVNLFVAFLPGAA
                     IGLLMHSFIKEYLFSPVPVALALVLGGIAILLIERWQPKSHISKLSDMSVPVALGVGL
                     AQVLALFPGVSRSGATIMGAYCLGLTRKAAAEFSFFLAIPTMIAAAGYDLLKSAGSLS
                     TGDIPVFAVGFVVSFVSAVLVVRVFVAFVARSSFAAFAWYRIAFGALLLIWYGRHSG"
     gene            complement(3174..4304)
                     /gene="bshA"
                     /locus_tag="VFB89_12470"
     CDS             complement(3174..4304)
                     /gene="bshA"
                     /locus_tag="VFB89_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HZM28154.1"
                     /translation="MRVGITCYPTYGGSGAVATELGLALARRGHEVHFITYDSPFRLR
                     GYAERVYFHQVETRMGRYPLFDHYPYTLALASKQHEVALREDLEVLHVHYAIPHATTA
                     YLAREMLRGTRELRVITTLHGTDITLVGQESSFYGITRFSIEQSDAVTAVSAFLRDET
                     YRAFSCVGCDVRVIPNFVNLEEYHPVAGPRTEALAPAGHKLITHVSNFREVKRVKDVV
                     RVFARISRAMPATLLMVGDGPDRPDAEKEAEELGVTSGVRFLGRLDSVETLLQATDLF
                     LLPSQTESFGLAALEAMACGAPVVASRAGGLPEVVDDGVNGILEPVGSVEAMGRRAIE
                     LLRDPNRHQAMRDAAVAKAQEFSAERVVPQYEALYQEVMAWT"
     gene            complement(4301..5227)
                     /gene="miaA"
                     /locus_tag="VFB89_12475"
     CDS             complement(4301..5227)
                     /gene="miaA"
                     /locus_tag="VFB89_12475"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HZM28155.1"
                     /translation="MAGANLPVIVGPTAVGKTGVVLALAQYLPLEVISADSRQVYRRL
                     DIGTAKPTLRERSRLAHHGLDLINPGERYSAGRFAKDAHDWISAIRARGRQPVVVGGT
                     GLYIKALVDGLFLEPPFDPARRGALQRWTAALDARELLRWADRLDPEFAGGGRQRAAR
                     AIEVVLLTGRPLTWWQIAAREESGVRPWYVHLTVPRAVLHQRISNRAQEMVRRGLIEE
                     VAAVLSEGHDARASGLDGIGIREVVEYLHGRRPRDTVADAIAMNTRRYAKRQETWFRH
                     QLSSPVLLLDATRSPEKLAAEIAAAWEGRVQA"
     gene            complement(5214..>5805)
                     /locus_tag="VFB89_12480"
     CDS             complement(5214..>5805)
                     /locus_tag="VFB89_12480"
                     /inference="COORDINATES: protein motif:HMM:NF020260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28156.1"
                     /translation="MSQDQPEFRAPLIQMFDTYVLYESSDGIVIVDQHSAHERIIYEL
                     AMDQLRGHGAAAQHLLLPITLELTDEELDALESYRDEIRAVGFETEPFGGRSVVLHAV
                     PSPHPRFDGGACFRETVADLARGRFGGWSNRLERFAASYACRAAIKAGQPLSEPEMRR
                     LLLRLFGTELPPHDVHGRATIVQLPREELERRFGRR"
BASE COUNT         1092 a         1962 c         1796 g          955 t
ORIGIN      
        1 agattctcgc gcacggcaga agggtcagca gcatcggcca tcgccagctc tgtatacccc
       61 acgattgcgc caaggatgtt attgaagtcg tgggcaatcc cacccgcgag cgtgccgatc
      121 gcttccatct tctgcgcttc atgcagctgg atttccaggc tcgcccgtcc ccgttccgcc
      181 tctttgcggt cggaaatgtc ccgcacctgg acctgcgtcg ccagctgtcc atcgaagacg
      241 accgggaggg cggacgcctc aacgtccacc agccggccct gcaggttgat cagctgaagc
      301 tccttgagcg gagtggcttc gccccgtccg ctcgtcatcc tctcggccac gatagcccgg
      361 actggcggtg cgatatgcgg taaaatctcc ctgccgagga taccgcttgc tgccgaggcg
      421 cccagtattc tcacgagctc gcgattggcg taggcgaagc gaccacctac gtcgacgtag
      481 atgccgacgg cggtgttttc cagcagcgat cggtaccgtt cttcactttc gcgtagtgca
      541 gcctccaccc tctgtcgcac ggccacatcg agctccagat tagcctcggt cttccgtagt
      601 tcggcagtca tgcgagtcgc cagcacctcc gcgcgctcac cgaccagcaa ttgcgcacgc
      661 aatgcaagag agacggccag gctgagtgcg attccgccta agaggatggc aaccgagccg
      721 cgggccacga tgccctgctc gaagtcaggc atcgtgctca gaaagatggt ccagggcctg
      781 ccggcggcat tcattcgctc cgttcgagtt aacgccggct gtcggcctgc tccggttgca
      841 accacgactg cgtcggcggc gtagagaatc ctgtctgctc gtggagttgg gccgtcgaac
      901 acctcgacgt ccagtccggg gacggcgccc agcataccgg caaccaggga gggagcgtgc
      961 acggccgcag cgaccagacc aaccatcgct gctcgacgac cggccaccgt tggaggcacg
     1021 acaccctcag ggtacaccgg cgccaatacc accaagctcg gagtaggagt gcgatccgag
     1081 gtcagggtca atcgcccgga gacgaccggc atacctgagt cccgagcccg ttcgagcgcc
     1141 tgtcggcgga ctggatcggt cgcaatgtcg aagcccaggc gttccgccgg tgcatcacgg
     1201 agcgactcgg catacagaat gggataggcg tctccggtgc cgcctgcagg ccagacctga
     1261 tagtcctgca atccttcgct ctgtacccgc cgcacgtgag cgaccagctc cgccgccggc
     1321 acccgctcga tgaactcgag gcccaccagc cctgggaatg cagtgtcggc ccctgcgtgg
     1381 tgtacatagt cacgccactc gctgcgggtt acttcttcgg atgcggcaaa gagacccgtg
     1441 gccgcccggg ccgcctggat acagaggcgg agccggtcag agagcgtggc gattgcgaga
     1501 tcgccccgat ggtcaaacgc aaccttcgca tcagcacgtg ctcgcgtcgc cgcagcccac
     1561 gcagcgccga tgctgattgc cacgcctagc gcaaacacag cccagggaag ccagctcgct
     1621 accggacggc gggtcatggt gcatccctcg cgagcaccac cgatcctgag ctgacccgga
     1681 cgatctgacc gtcaggcttg agaacacgca gggcgccgtc cggctcgatg ccggccgcac
     1741 gtccccgctc aggctcggcc aactcacgtc ccagcagcca atcccgttgc ccgaacgcgc
     1801 gcagctctgc agcggagagg ccgcttccgg tttcggccag tggcagcagt gaaccgacca
     1861 gcggccacag gatgtggtcg ggctccagat cctgccgcca gtcggccagg cggccgggag
     1921 cgaccctcgc atctgtgggg agtggattcc gcacgttcag tccaagtccc accacgaccc
     1981 aggcgaggag atcaccatgc caccgagctt cgcacagtat gccaccaacc ttgcgatcgt
     2041 tgacgatcac atcgttgggc cacttcagcg atgctggcgg cagccctccg acctcggcca
     2101 gcgcttcggc ggccgcgaga ccaaccctga ggctgagcac ttcgacgccg ggcagacggg
     2161 ttggccgaca caaaaaactg agccacagtc ccccgaccgg cgaggtccag ttgcgtcccc
     2221 gggttccccg cccctcgacc tgctcggcgg cgactacgac cgtgccggcc ggcgcgcctt
     2281 cggccgcgag gcggtgagcc tcgtcctggg tcgagggaag ccgctcgtag cgatgaaccg
     2341 tttgcatcga tcgagctatc cggaatggcg gccgtaccag atcaggagaa gtgctccgaa
     2401 ggcgattcgg taccaggcga atgccgcaaa gctggagcgg gcgacgaatg ccacgaagac
     2461 acgaaccacg aggactgcgg acacaaacga gaccacgaat cccacggcaa acaccggtat
     2521 gtccccggtc gacagagacc ccgcgctctt gaggaggtcg tatccggccg ccgcgatcat
     2581 tgtcgggatc gcgagaaaga atgagaactc cgccgctgcc ttgcgagtaa ggcccaggca
     2641 gtacgccccc atgatggtgg cgccggagcg ggagacacct ggaaagagcg ccagcacctg
     2701 ggccagccct acgccaagcg ccaccggcac cgacatgtcg ctgagcttgc ttatgtgaga
     2761 cttgggttgc caccgttcga tcaggaggat ggcgattcca ccgagcacca gagcgagcgc
     2821 gaccggaacc gggctgaaaa ggtactcctt gatgaacgaa tgcatcagaa gcccgattgc
     2881 ggcccccgga agaaacgcta cgaacaggtt caccacgagc tgatggcgga ggatgccact
     2941 cttgtgcgtc tcgatcgctc gagccacgtc ggaccggtac agccacacga tggcgagtat
     3001 ggcgcccagc tgaatgaaga tctcgaacgc tttggcgctc tcgccggtaa aggcaagcca
     3061 gtcgccggca acgatcagat ggccggtgga tgacaccgga atgaattcgg tagcgccttc
     3121 gactaccccc aggatcgcgg ccttgacgag tgggtggagc tgggccagcg ggctcaggtc
     3181 cacgccatga cctcctggta gagcgcctcg tactgaggca ccactcgctc ggccgagaac
     3241 tcctgcgcct tggcgacggc cgcgtcgcgc attgcctggt ggcgattggg atctcgcagc
     3301 agctcgatcg cccgacgccc catcgcttcg accgacccga ccggctcgag aatgccgttg
     3361 acgccgtcat ccaccacctc aggaagcccc ccagcgcggc tcgcaaccac cggcgcaccg
     3421 caggccatgg cctccagcgc cgccaagccg aacgattcgg tctgcgatgg aagcagaaac
     3481 agatcggtgg cctgaagcag agtctccacc gaatcgagcc ggcccaggaa tcgcacgcca
     3541 ctggtcacgc ccagctcttc tgcttctttc tccgcatccg gccggtcggg gccgtccccc
     3601 accataagca gggtcgcggg catggctcga ctgattcggg cgaacactcg gactacgtcc
     3661 ttcactcgct tcacttcccg gaagttggac acatgggtaa tgagcttatg gccggctggg
     3721 gcgagcgctt ctgttcgagg cccggcgacg gggtggtact cctcgaggtt cacgaaattc
     3781 ggaatgactc gtacatcaca gccgacacag ctgaacgccc ggtaggtctc gtcgcgcaga
     3841 aaggcggaga cggccgtaac cgcgtcggac tgctcgatgg aaaagcgggt aatcccgtag
     3901 aagctcgatt cctgcccgac cagcgtgatg tcggtgccgt gcaaagtcgt gatcacccga
     3961 agttcccgcg taccccggag catttcgcgc gcgaggtagg cggtggtggc gtgcgggatt
     4021 gcatagtgca cgtgcaaaac ttccaaatct tcccgaagcg ctacctcgtg ctgcttcgag
     4081 gccagcgcga gcgtgtaggg ataatgatcg aacagcgggt agcgccccat ccgcgtttct
     4141 acctggtgga agtaaacccg ctcggcgtag ccccgaagcc ggaaaggaga gtcgtaggta
     4201 atgaaatgga cttcatgccc gcgacgtgcc agcgccaaac cgagctcggt tgccacggct
     4261 cccgacccac cgtaggtcgg atagcaggta atcccgactc tcatgcctgc acccttccct
     4321 cccatgccgc tgcgatttcg gcggcgagct tttcgggcga ccgcgtggcg tccaggagca
     4381 gcaccgggct cgaaagctgg tgccggaacc acgtctcctg ccgtttggca tagcgtcgcg
     4441 tattcatggc aatcgcatcg gcgacggtgt cacggggccg ccgcccatga aggtactcga
     4501 ccacctcccg gatgccgatg ccatcgagcc cggaggctcg tgcgtcgtga ccttccgaca
     4561 gcaccgctgc gacttcctcg atcaggcccc ggcgcaccat ttcctgcgcc cggttgctga
     4621 tgcgctgatg cagtactgcc cgaggaaccg tcagatgaac gtaccacggg cgcacgccac
     4681 tctcttcacg cgccgctatc tgccaccagg tcagcggacg tcccgtgagc agcacgacct
     4741 caatcgcgcg cgcggcccgt tgccgcccac cccccgcgaa ctcggggtcg aggcggtcgg
     4801 cccaccgaag cagctccctc gcatcgagcg ccgcggtcca ccgctggagc gctccccgtc
     4861 gcgcaggatc aaagggcggt tcgaggaaca agccgtccac cagcgctttg atgtaaagtc
     4921 cggtcccgcc cacaaccacc ggctgtcgac cgcgcgcgcg gatcgcgctg atccagtcgt
     4981 gagcatcctt ggcaaatcgc ccggcactgt aacgctcgcc cggatttatc agatccaggc
     5041 catgatgcgc cagacgtgag cgctcgcgca gcgtcggttt ggcggttccg atgtccagtc
     5101 gccggtagac ctgtcgggag tcggcggaaa tcacctcgag gggcagatac tgggcaagcg
     5161 cgagcacaac gcctgtcttg cccacagcgg tagggccgac gatgaccggg aggttagcgc
     5221 cggccaaacc ggcgctccag ctcctcgcgg ggtaactgca cgatcgttgc ccggccgtga
     5281 acgtcgtgcg ggggaagctc agtcccgaac aggcggagca gcagccgccg catctccggc
     5341 tcgctcagcg gctgaccagc cttgatggct gcgcggcagg catagctcgc cgcgaatcgt
     5401 tcgagccgat tggaccagcc tccgaaacgt ccgcgcgcca ggtcggcgac cgtctcccga
     5461 aagcaggctc ctccatcgaa ccttggatgc ggagacggca cagcgtggag caccacgctg
     5521 cgtccgccaa agggttccgt ctcgaatccg accgcgcgga tctcgtcccg gtagctctcc
     5581 agtgcgtcca gctcttcatc tgtgagctcg agagtgatag gaagcagcag gtgctgggcc
     5641 gccgcaccgt gtccccgcag ctgatccatg gccagctcgt agatgatacg ctcgtgagcc
     5701 gagtgctggt ctacgatcac gatgccatcg gaggactcgt atagtacgta ggtgtcgaac
     5761 atctggatca gcggcgcgcg gaattccggc tgatcctgtg acatc
//
LOCUS       DASRJQ010000545         2537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_683270, whole genome shotgun sequence.
ACCESSION   DASRJQ010000545 DASRJQ010000000
VERSION     DASRJQ010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683270"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VFB89_12485"
     CDS             complement(<1..751)
                     /locus_tag="VFB89_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28157.1"
                     /translation="MIAGKLGRRLGATALLALLPSGVALAQCPDGTPPPCRAAGPAPA
                     VRRPPPPLDERTWIVVPFENVARVADIDWLKNASVNLLYLDMSKWRDIRVIDDERVAD
                     LIREVPEARGGEQISLQSGIAVAKRAGAGKLVMGDLLKVGTRTQVVGKVFDVRTGQRL
                     RTVRQEASNPDSIMGAFGQLARAVLNVAPPAGTSLGTIGTTSVGAYQAYLGGVGFLNR
                     WLLDSAHTQFAKAITLDSSFALAHYKLALVYG"
     gene            complement(748..>2537)
                     /locus_tag="VFB89_12490"
     CDS             complement(748..>2537)
                     /locus_tag="VFB89_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28158.1"
                     /translation="RSGRQLRLVREQLSNLASLDSLTAGFGRVARGALAVPGPAISRE
                     GVGTTSLEAYAAYLSGLEALNGIQIDSARARFLRATRIDSTFALAHLRASQTHIRSTS
                     VPGSDPNRAAALRFAATLPPRERSMLQAADASTRADYGELCRLARGLVATDSSDAEAW
                     NLLSSCHQDVRLVIGPDGDSSRAVVRGDLSAALFAARRSYALDPTGPWAGALPNLLSI
                     GAGPQCLSGVTGVCPPRQLYATTGWVTGDSVRYSGLRWSDVKHAPPYMSAAWVSQYQE
                     RLREAREIYRRLVASKPDNWLLEWILARSSLSVGDTAAAASGFARVLAIGPDSAPMFM
                     LADRRDLALARQRPAEAAALADSVLAFPPALRNYSYFSMLGRPSEGADTTIGSRQAAA
                     WRPILLGVLPLGFDSLETAIAARLSGPQRDDFLQLSTLAAYHMRRTGPALDTAARHPV
                     KRFQAWLARGDSSRARAALAEFDKELLARHAATFDDGGWLFNAESHLELGDSATALAR
                     LVEWERRWVFLSRESMILEQYYWQRTTPRLFGRTWLLYGDLARAAGRRAEAGRAYQMV
                     AGLWANGEPPVQPAVQRARDALKQLGFRP"
BASE COUNT          418 a          836 c          925 g          358 t
ORIGIN      
        1 agccatacac cagcgccagc ttgtagtgcg ccagcgcgaa ggaagagtcg agggtgatgg
       61 ccttggcgaa ctgggtatga gcggagtcga gcagccagcg attcaggaat ccgacgccgc
      121 cgaggtatgc ctgataggca cccaccgagg tggtgccgat ggttcccagg ctggtgccgg
      181 ccggcggggc gacgttgagc acggcgcgcg ccagctggcc gaacgcgccc atgatggaat
      241 cgggattcga ggcttcctgg cgcaccgtcc tgagccgttg gcctgtccgg acgtcgaaca
      301 ctttgccgac cacctgggtc cgggtgccca ctttgagcag gtcacccatc accagcttgc
      361 cggcgccggc gcgtttggcc accgcgatgc ccgattggag cgagatctgc tcgccgccac
      421 gcgcctccgg tacttcacga atcagatccg ccacgcgctc atcgtcgatg acccggatgt
      481 cgcgccactt ggacatgtcg agatacagga gattgacgct ggcgttcttg agccagtcaa
      541 tgtccgccac ccgcgccaca ttttcgaagg gaacgacgat ccaggttcgc tcatccagtg
      601 gcggtggagg ccgtcggacc gcgggggcgg gaccggcagc gcgacacggt ggcggggtgc
      661 cgtcggggca ctgagcgagt gccacaccgg acggcaggag ggccagcagc gcagtggccc
      721 cgagtcggcg gccgagcttt ccagcgatca tggccggaaa cctagctgct tcaatgcgtc
      781 acgggcccgc tggacggcgg gctgtaccgg gggctcaccg ttggcccaca atccggccac
      841 catctgatag gccctgcccg cctcggcccg gcgcccggcg gcccgggcca ggtctccgta
      901 cagcagccag gtgcggccga acagcctcgg ggtcgttcgc tgccagtagt actgctcgag
      961 gatcatggac tcccgcgaga gaaacaccca acggcgttcc cattctacga ggcgggcgag
     1021 tgccgtggcc gaatcgccga gctcgaggtg ggattcggcg ttgaacagcc agccgccgtc
     1081 atcgaaggtt gcggcgtggc gcgccagcag ctccttgtcg aactcggcga gcgccgcgcg
     1141 ggcccgcgac gaatcgcccc gagcgagcca ggcctgaaac cgtttgacag gatgccgagc
     1201 cgccgtgtcg agcgccgggc cggtccggcg catgtggtac gccgcgagcg tggaaagctg
     1261 gagaaaatcg tcccgttggg gtccggagag ccgcgcggcg atcgcagtct cgagcgagtc
     1321 gaagcccagg ggcagcacgc cgagcaggat gggccgccac gccgcggcct ggcgcgagcc
     1381 gatggtcgtg tcggctccct cggacggccg gcccagcatg ctgaaatagg agtaattgcg
     1441 gagggccggc gggaaggcca gcaccgagtc ggcgagcgcc gcggcttctg caggacgctg
     1501 gcgcgccagc gcgagatcgc gacgatctgc cagcatgaac atcggggccg agtcggggcc
     1561 gatcgccagc accctggcga atccgctcgc ggcagcggcg gtgtcaccca cactgaggga
     1621 gctgcgcgcc aggatccact ccagcaacca gttgtcgggc ttgctggcga ccaaacggcg
     1681 gtaaatctcc cgcgcttccc gcagccgctc ctggtactgg gatacccagg cggccgacat
     1741 atacggcgga gcgtgcttga cgtcggacca gcggagcccg gagtagcgca ccgagtcgcc
     1801 ggtcacccag cccgttgtgg cgtagagctg ccgtggcggg cagactccgg ttactccgga
     1861 gaggcattgg ggcccggcgc cgatggacag caggttgggc aaggcgccgg cccaggggcc
     1921 ggtcgggtcg agtgcgtagg accggcgcgc cgcgaagaga gccgcgctca gatcgcctcg
     1981 tacgaccgcc cggctgctgt cgccatcggg gccgatgacc agccggacgt cctggtggca
     2041 gctcgagagc aggttccagg cttccgcatc cgaggaatcg gttgccacca aaccccgggc
     2101 gagccggcag agctctccat agtcggcgcg agtactcgcg tcggcggcct ggagcatcga
     2161 acgctcgcgg ggcggcagcg tggccgcgaa gcgcagggcg gcggcgcggt tgggatcgct
     2221 cccgggaacg gacgtggacc tgatgtgggt ctgcgaggcg cgcaggtggg cgagcgcaaa
     2281 cgtgctgtcg atgcgggtcg cccgcaggaa ccgggcgcgc gccgaatcga tctggatgcc
     2341 gttcagtgcc tcgagccccg acaggtaggc cgcgtaggct tcgagcgatg tcgtgccgac
     2401 tccttcccgg gaaatggccg gtcccgggac cgcgagcgcg cctcgcgcca cgcggccgaa
     2461 ccccgcagtg agcgagtcca gcgaggcgag attgctcagc tgctcgcgga ccagccgaag
     2521 ctgtcggccg gatcgga
//
LOCUS       DASRJQ010000546         6391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2486621, whole genome shotgun sequence.
ACCESSION   DASRJQ010000546 DASRJQ010000000
VERSION     DASRJQ010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6391
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2486621"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VFB89_12495"
     CDS             complement(<1..466)
                     /locus_tag="VFB89_12495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZM28159.1"
                     /translation="MDDRLLIARVQAGDASAARELYDAHVDRVYRLVYRLAGDVDQAK
                     DYTQEAFIRVFDRLGHFRGESSLGTWICSVALSVALNGLRKIKRLRTREVALDGAMEL
                     GSTPREAEPDLKSRLARAIDQLPEGYRSVFLMHDVEGYTHEEIGQLLGVQPGT"
     gene            613..795
                     /locus_tag="VFB89_12500"
     CDS             613..795
                     /locus_tag="VFB89_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28160.1"
                     /translation="MRFEHSGSGAPAEARKVECLRDKQGQFIGRILEPQSDRLVGYGA
                     ATVLLTRPQPTGSRCS"
     gene            969..3458
                     /gene="gyrA"
                     /locus_tag="VFB89_12505"
     CDS             969..3458
                     /gene="gyrA"
                     /locus_tag="VFB89_12505"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HZM28161.1"
                     /translation="MTAPNSRERILPRLIEEEMQQSFINYSMSVIVSRALPDVRDGLK
                     PVHRRILYAMNELGLVPGRPYKKSATVVGDVLGKYHPHGDASVYDALVRMVQEFSLRN
                     PLIDGQGNFGSVDGDPAAAYRYTEARLTPVAIAMLEDIDKNTVDFQPNFDDRLQEPTV
                     LPAKIPNLLVNGSSGIAVGMATNIPPHNLREVAKAVELLVDDPEATIAELRKAIKGPD
                     FPTGAYIYGREGIKEAYETGRGRVVMRARAQIEEKASSGRYQIVVTEIPYQVNKENLV
                     KSIAELAVEKKLEGISGVNDESDKEGMRVVVELKRDANPNVVLNQLYKHTAMQSTFGV
                     IMLALDHGAPKVMNLKQLLERFVEHRHDIIVRRTRFDLEAAEAREHILEGLKIAVDNI
                     DDVVDIIRKSKDVPDADARLRKRFGLSEKQSDAILNMRLAKLTGLEIEKLEAELKEVR
                     ATIKELKGILASKPKRMAILKGEMAEITDKFGDDRRTEIVADQGEFTVEDLIAEEDMV
                     ITISHSGYIKRISISTYRRQRRGGRGLNGADLKEDDWVEHLFIASTHDYLLVFTNNGQ
                     LYWLKVHEIPQAGRAARGKPIVNCIAIKPDEQIAALVPVREFSADQFILFATRQGTVK
                     KTALSEYGNVRTNGIRAINIEQDDELIDAQICDTTSDVVLATREGMSIRFHQGDVRDM
                     GRVATGVKGIELEKSDAVIGMVVIRRDATLLVVSEHGFGKRSELSEYRVQKRGGKGII
                     TFKVTDKTGACVALKEVQPDDELMMITRHGIIIRVPVEGIRVIGRNTQGVKVMNLGSG
                     DAVVDVARVVKEDESGTDEPAGEEVAPSAED"
     gene            3518..4411
                     /locus_tag="VFB89_12510"
     CDS             3518..4411
                     /locus_tag="VFB89_12510"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HZM28162.1"
                     /translation="MNRTPLVEIPLAGRTFRLKCEQLQPIGAFKLRGAYTAVARLGDR
                     RRSGIVTPSSGNHGFAIAWSARAFGIRAVVVMPEAAARMKVEAVRGVGGEVVFAGRTR
                     SPEQGARAEAMAAAEGLNLIPPFDHPDVIAGQATCGLEILEDAPDVDTILVPTGGGGL
                     LAGISAAVSALKPAVRVIGVEPEGAAKLSAALAAGKPVALERTESLADGLLARAIGDL
                     TWQVIRPVVREAVRVTEVEIALAVKHLYRQVQLVIEPSGAVTTAALLAGRVSAEESVV
                     AIATGGNVDPGLFNQLVHASA"
     gene            4408..5838
                     /locus_tag="VFB89_12515"
     CDS             4408..5838
                     /locus_tag="VFB89_12515"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZM28163.1"
                     /translation="MSPAPKILVADDDPALSRTLWWILKENGYDVTTVPGGEHLLERL
                     ETEPFDLVLLDIMMPKVDGLELLGRLKGDDRFKDLPVLMISSMPPEEATVRSLGLGAN
                     DFIPKPFRMKELLARVKAQLRVGRQLNQARLEARTRSAMMDIMTEVTESLKPDEIYQI
                     LVRRVAQALNISRCSIILSGPEEQTATVVAAYENSTLHNLPIDLRRYPEIHHCLTTGE
                     TVLARDVCTDPLYQEVRDAWEAEGRAVPTRSAICIAFSLKDHPAGVFFLRTVSEDGPL
                     TDHDLEFAGQVIRSAVAALEKAYDLEHAVLGQEQLRRLAETDPLTAVYNRRALMQKLE
                     QEMDRAARYAARLTAMMIDIDNFKHINDTHGHLVGDKVLKQLANLLKREQRSVDILAR
                     YGGEEFVVLLPETTVAESRNFAERILRRVATYDFGDAGHAVRVTISVGMASYPDARVT
                     DGQSLLKLADSHLYRAKSDGRNRFSD"
     gene            5835..>6391
                     /locus_tag="VFB89_12520"
     CDS             5835..>6391
                     /locus_tag="VFB89_12520"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM28164.1"
                     /translation="MSNRRCPRCHTNYAAPARFCPRDGTPLVEVAPAPDDGANTAKLR
                     AVEKAAEAADPPYTSLTGQTLGEHYLVDRRLGEGGMSYVYLAQELATGNKVALKVLSP
                     KLSRDPGSVERLRREAAIAMRLSHPNVCPILGFGETPHPLIYLVMPYLPGEPLSDHEI
                     RRGPMDIDEAIPLLVQMCRGLEHAHG"
BASE COUNT         1276 a         1991 c         2057 g         1067 t
ORIGIN      
        1 acgttcccgg ttgcactccg agcagctgac cgatttcctc gtgggtgtaa ccctctacat
       61 cgtgcatgag gaacaccgac cgatacccct cagggagctg gtcaatcgcc cgcgccagcc
      121 gggacttgag atcgggctcc gcttcacggg gcgtcgagcc cagctccatg gcgccgtcca
      181 gcgcgacctc gcgggtccga agccgcttga tcttgcgcag gccgttcagg gcgaccgaca
      241 gagcgactga acagatccag gtgccgagcg atgactctcc acgaaagtgg ccgaggcggt
      301 caaacacgcg aatgaaggcc tcctgggtgt agtccttagc ctgatccaca tcgccggcga
      361 gccgatacac caggcggtag acccgatcga cgtgtgcgtc gtacagctcg cgcgcggcgc
      421 tcgcatcacc ggcctgtaca cgggcgatca gaagtcggtc gtccaccggg ctccggtcga
      481 gggtcggact cgtccctgtc gtcggtcgtc tgacactcaa gctcacaaga gagttgcgtc
      541 tggaccgggc cggcgcaacc aatccgcagc tgagagcatc tcaggttcgt aaccggtcat
      601 cgagaggaac tcatgcggtt tgagcacagc ggatccggcg cgcccgccga ggcccgaaag
      661 gtcgaatgcc tccgagacaa gcaggggcaa ttcatcggca ggattctcga gcctcagtcg
      721 gaccggctgg tcggctacgg tgctgcgacg gtgctcctga cacgccctca gcccactgga
      781 tcgcgctgct cgtagcggct cgttcgagcc gccccagaag ggcccactaa cacacctcct
      841 aaagccaagc accacaatcg gttgtattca gcggccgcga ttgcggccgc cgccgggtcc
      901 cggatatatt tatgaggtac cgcggccggt cccgggccgc tcgcgcttct cgccaccgcg
      961 actaactcat gaccgctccc aattcgcgtg agcgcatcct gcctcggctc atcgaggaag
     1021 agatgcagca gtcgttcatc aactactcga tgagcgtcat cgtctcccgc gcgctgccag
     1081 acgtgcgcga tggattgaag ccggtgcacc gtcgcatcct gtacgcgatg aatgagttgg
     1141 gcctggtgcc gggaaggcct tacaagaaat cggcaaccgt agtgggtgat gtcctgggca
     1201 agtaccaccc gcacggcgac gccagcgttt atgacgcgct ggtacgcatg gtacaggagt
     1261 tctcgctccg gaacccgctg atcgacgggc aaggcaattt cggatcggtg gacggcgatc
     1321 ctgcagcggc ctaccgatat accgaagccc gcctgacacc ggttgccatc gcaatgctgg
     1381 aggacatcga caagaacacg gtcgatttcc agcccaactt cgatgatcgg ctccaggagc
     1441 cgaccgtcct gccggccaaa attcccaacc tgctggtcaa cggctcgagc ggcatcgcgg
     1501 taggcatggc caccaacatt ccaccgcaca atctgcgtga ggtggccaag gcagtggagc
     1561 ttctggtgga cgatcccgag gcgactattg ccgaactgcg gaaggcgatc aagggtcccg
     1621 actttccaac cggcgcctac atctatggac gcgagggcat caaggaggcg tacgagaccg
     1681 ggcgtggccg cgtggtcatg cgggcgcggg cacagatcga agagaaagcg tcgagcggca
     1741 gatatcagat cgtggtgacc gaaatccctt accaggtgaa caaggagaac ctggtcaagt
     1801 caattgccga gcttgctgtc gagaagaagc tggaggggat cagcggggtc aacgacgagt
     1861 ccgacaaaga agggatgcgg gtcgtcgtcg agctcaagcg agacgccaat cctaacgtcg
     1921 tcctgaacca gctgtacaag cacactgcga tgcagtccac cttcggcgtg atcatgctgg
     1981 cgctcgatca cggtgcgccg aaggtgatga acctgaagca gctgctcgag cggttcgtcg
     2041 agcaccggca cgacatcatc gtgcgccgga ccaggttcga cctcgaggcg gcggaagccc
     2101 gcgagcacat cctcgagggc ctcaagatcg cggtcgacaa tatcgacgac gtggtcgaca
     2161 tcatccgcaa atccaaggac gtgcccgatg cggacgcgcg tctgcggaag cggttcggcc
     2221 tgtccgagaa gcagagcgac gcgatcctca acatgcggct ggccaagctc acgggcctcg
     2281 aaatcgagaa gctcgaagcc gaactcaagg aagtccgcgc gaccatcaag gagctgaagg
     2341 ggatcctcgc atccaagccg aagcggatgg caattctcaa gggtgagatg gccgagatca
     2401 ccgacaagtt cggtgacgac cgccgcaccg agatcgtcgc cgatcagggc gagttcacgg
     2461 tggaggatct gatcgccgaa gaggacatgg tcatcaccat ctcccactcc ggctacatca
     2521 agcggatctc gatctccacc tatcgccgcc agcgacgggg cggccgtggc ctcaatggag
     2581 ccgacctgaa ggaagacgat tgggtcgagc acctttttat cgccagtacc cacgactatc
     2641 tgctcgtctt caccaacaac gggcagctct actggctcaa ggtgcacgaa attccgcagg
     2701 ccggtcgagc ggctcgaggc aaaccaatcg tcaactgcat cgcgatcaag ccggacgagc
     2761 agatagccgc actggtcccg gtccgcgaat tctcggctga tcaattcatt ctctttgcga
     2821 cccgccaggg cacggttaag aagacagcgc tctcggagta cggaaacgtc cgcaccaacg
     2881 ggatccgggc catcaacatc gagcaagacg acgagctgat agatgcgcag atctgcgaca
     2941 ccaccagcga cgtcgtgctc gcgacgcggg aagggatgag catccggttc catcagggcg
     3001 acgtgcgcga catgggccgg gtggcaaccg gagtgaaggg aatcgagctg gagaagagcg
     3061 atgccgtgat tggcatggtc gtcatccggc gtgacgccac gctgctggtg gtgagcgagc
     3121 acggcttcgg caagaggagc gagttgagcg agtaccgggt ccagaagcgc ggcggtaagg
     3181 gaatcatcac gttcaaggtg accgacaaga ccggcgcctg cgttgcgctc aaggaagtgc
     3241 agccggacga cgagctcatg atgattaccc gacacggaat catcatccgg gtgccggtcg
     3301 aaggtatccg ggtgatcgga cgcaacaccc agggcgtgaa ggtgatgaat ctggggtccg
     3361 gagacgccgt agtggacgtc gccagggtgg tcaaggagga cgaaagcggg accgacgagc
     3421 ctgccggcga ggaagtagcg ccgagcgccg aggactgagg cgactgggcc cgtcgctgga
     3481 ggacatccgg gacgcggccc gcacgctcgc gggggtcgtg aaccgcaccc cgctggtcga
     3541 aattccgctc gccgggcgaa ccttccggct caagtgcgag cagttgcaac cgatcggagc
     3601 cttcaagctc cgcggggcgt acaccgccgt agcccggctg ggcgatcggc gccggagcgg
     3661 gatcgtcacg ccctcgagcg ggaatcacgg ctttgcgatc gcgtggagcg cgagggcgtt
     3721 cggaattcgc gccgtagtcg taatgccgga agcagcggca cgaatgaagg tcgaggcggt
     3781 tcgtggggtg ggtggcgagg tcgtgttcgc cggccgaact cgctcgccag agcagggagc
     3841 acgggccgag gccatggcgg cagccgaggg gctcaatcta attccgccct tcgaccatcc
     3901 tgatgtcatc gctggtcagg cgacctgcgg gctcgagatt ctagaggacg cgccggacgt
     3961 cgacaccatc ctggttccca ctggcggagg gggtctgctc gcgggaattt cggcggcggt
     4021 ctccgcgctc aagccagcgg tacgtgtcat cggggtcgag ccggaaggag cggcaaagct
     4081 ctcagcggct cttgccgccg gtaagccggt ggctctggag cgaaccgaga gcctggcgga
     4141 tggactcctg gctcgcgcca tcggcgacct gacctggcag gtcattcgcc cggtggttcg
     4201 ggaggccgtt cgggtgaccg aggtggagat cgcgctcgct gtgaagcatc tttatcggca
     4261 agtccagctg gtcatcgaac catccggcgc ggtaaccacg gccgcgctac tcgccggacg
     4321 cgtgagcgca gaggaatccg tcgtagccat tgcgaccggg ggcaacgtag acccgggcct
     4381 cttcaatcaa ttggtgcacg cgagcgcatg agccctgccc ccaagatcct cgttgcggac
     4441 gacgatccgg ccctctcccg caccctctgg tggattctca aggagaatgg gtacgacgtc
     4501 accaccgttc ccggcggcga gcatcttctg gagcgactcg agaccgagcc tttcgatctg
     4561 gtcctcctcg atatcatgat gccgaaggtc gatggcctcg agctgctggg ccggctcaag
     4621 ggagacgacc ggttcaagga ccttcccgtc ttgatgatct cgtcgatgcc gcccgaggag
     4681 gccaccgttc gctccctggg tctcggcgcc aatgacttca tcccaaagcc gttccggatg
     4741 aaggagctgc tggcccgggt caaggcgcag cttcgagttg gccggcagct gaaccaggcc
     4801 cgtctcgagg cccggacccg atcggccatg atggacatca tgaccgaggt cacggagtcg
     4861 ctcaagccgg atgagatcta ccagatcctg gtgcggcggg tggcccaggc gctcaacatt
     4921 tcgcgctgct cgattattct gagtgggccg gaagagcaga cagcgaccgt agtggccgca
     4981 tatgaaaact ccacactgca caatctgccg atcgatctcc ggcgctatcc ggaaatccat
     5041 cattgcctga cgaccggtga gaccgtgctc gcgcgagacg tctgcaccga cccgctttat
     5101 caggaagtgc gggacgcgtg ggaagcagaa ggtcgggccg tgcccacccg ttcggcgatc
     5161 tgcatcgcgt tctcactcaa ggatcatccc gccggcgtct tcttcctgcg aaccgtgtcc
     5221 gaagatggac cactgaccga tcacgacctc gagtttgccg ggcaggtaat ccggtcggcg
     5281 gttgcggctc tggagaaggc gtacgacctg gagcacgctg tgctgggtca ggagcagttg
     5341 cggcgactgg ccgagaccga tccactcact gccgtctaca accggcgagc cttgatgcag
     5401 aagctggagc aggaaatgga tcgcgcagcc cgttacgccg cgcgactcac ggccatgatg
     5461 atcgatattg acaacttcaa gcacatcaat gacacccacg gccacctcgt gggcgacaag
     5521 gtgctgaagc agcttgcgaa cctgctcaag cgggaacagc gttcggtgga tatcctggcg
     5581 cgctacggcg gggaggaatt cgtggtgctg ctcccggaaa cgaccgtggc tgagtcgcgc
     5641 aactttgccg agcgtattct gcgccgcgtg gcgacgtatg acttcggtga cgcgggccac
     5701 gcggtacgag tcactattag tgtcgggatg gcgtcctatc cggatgcacg cgtcaccgac
     5761 ggccaatccc tgctcaagct ggctgacagt cacctatatc gcgccaagtc tgacggccgt
     5821 aaccgattca gcgattgagc aaccgccgct gtccgcgctg ccacaccaac tatgccgcgc
     5881 cggcgcgatt ctgcccccgc gacggaactc cgctcgtgga ggtagccccg gcaccggatg
     5941 acggtgccaa cacggcaaag cttcgcgcgg tggagaaggc ggctgaagcc gcggaccccc
     6001 cctacaccag cctcaccgga cagacacttg gagagcacta cctggtagat cgccgcctgg
     6061 gcgagggcgg aatgtcgtat gtctacctcg cacaggagct cgccaccggc aacaaggtcg
     6121 cactcaaagt gctttccccg aagctgagcc gggacccggg atcggtggag cgcctccgcc
     6181 gcgaagctgc gatcgccatg cggctttccc acccgaacgt ttgtccgatt ctcggcttcg
     6241 gcgagacacc ccatccgctc atctacctgg tgatgccgta tttgcccggg gagccgctga
     6301 gcgaccacga aattcgccgc ggccccatgg acatcgacga ggccattcca ctgctggtgc
     6361 agatgtgccg ggggctggag cacgcgcacg g
//
LOCUS       DASRJQ010000547         2024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_929812, whole genome shotgun sequence.
ACCESSION   DASRJQ010000547 DASRJQ010000000
VERSION     DASRJQ010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929812"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1674)
                     /gene="asnB"
                     /locus_tag="VFB89_12525"
     CDS             complement(<1..1674)
                     /gene="asnB"
                     /locus_tag="VFB89_12525"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HZM28165.1"
                     /translation="MCGIAGVFAYEASAPPVDEAELLRVRDAMGRRGPDGAGLWLSPD
                     QRAGLAHRRLAIIDLSATGAQPMTTADGRLTITFNGEIYNYKELRRNLDSSGVRLRSE
                     SDTEVLLHLVALHGPRALEMLRGMFAFAVWNADDRSLLLARDSFGIKPLYYADDGCTV
                     RFASQVKALLRAEGVDTTPNPAGSVGFFLWGSVPEPHTLFEGIRALPAGSHVTYRPGN
                     PGQPRRFFSIREELASAADASQASDEQEFTWMREAVGDSVRHHLVADVPVGVFLSAGL
                     DSTTVTAIASEVEGSDLRTLTLGFREFRGTGQDEVPLAEEVARRYGTRHSTSWVSEAD
                     FNAALPDLLAAMDQPTTDGVNTYFVSRAGAQAGMKVALSGLGADELFGGYPGFRQVPR
                     MASSLRPFRAFPLLGRATRRITEPLLRRFTSPKFAGLVEYGGSLAGAYLLRRALYAPW
                     EVDRFMDREAVKEGLERLDTLSALSGTVDGLTTRHQKMVALELSWYMRNQLLRDADWA
                     GMAHSLEIRVPFVDVQFFRRVAPLLARAKPPTKRSVASTPRSALPQALLR"
     gene            1814..>2024
                     /locus_tag="VFB89_12530"
     CDS             1814..>2024
                     /locus_tag="VFB89_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28166.1"
                     /translation="MSLQHDPPPGLSNRGRQCFGAHRQDNTALACKDLNELQLMVIDA
                     LAKRPSARHDHWNSSRMQEVEERSST"
BASE COUNT          386 a          675 c          624 g          339 t
ORIGIN      
        1 ccgaagcagc gcctgtggca aggcagaccg cggcgtgctt gcaacggacc tcttggtggg
       61 cggcttcgcg cgcgcgagca gaggcgcaac tcggcggaag aactgcacat ctacgaaggg
      121 cacgcggatc tccagagagt gagccatgcc cgcccagtcg gcgtcccgaa gcagctggtt
      181 tcgcatgtac caagagagtt cgagggccac catcttctga tgccgggtgg taagtccatc
      241 tacggtgccg ctcagtgccg acaaggtgtc gagtcgctcc agtccctcct tgaccgcctc
      301 acgatccatg aagcgatcga cttcccaggg tgcgtagagt gcgcgccgaa gcaggtaagc
      361 cccggcaagg ctgcctccat actcgacgag accagcgaat ttgggagagg tgaaccgcct
      421 cagcaggggt tctgtgatcc gccgggtcgc ccggccaagt agcgggaaag ccctgaaagg
      481 ccgaagcgac gacgccatgc ggggcacttg cctgaagccc gggtagccgc cgaagagctc
      541 gtcagccccc agccctgaga gcgctacctt catcccggcc tgggcgcccg cccggcttac
      601 gaagtacgtg ttgaccccgt ccgtcgtcgg ctggtccatc gccgccagga gatctggcag
      661 ggccgcgttg aaatcggcct ccgagaccca tgacgtgctg tgtcgcgtcc cgtagcggcg
      721 cgccacctct tcggcgagcg gcacctcgtc ttgcccggta ccccggaatt cacggaatcc
      781 cagagtcagc gtcctcaggt cgctcccttc aacttcggag gcaatagctg tgacagtcgt
      841 ggaatcaagt cccgccgaca ggaacacacc gacaggcacg tccgcaacga gatggtgtcg
      901 aaccgagtcc ccgaccgctt ctctcatcca ggtgaactcc tgctcatccg aggcctgaga
      961 cgcatccgcc gcagacgcca actcttccct tatcgagaag aagcgacggg gctggcccgg
     1021 gttccctgga cgatacgtga catgcgatcc ggcggggagc gctcggatcc cctcaaacaa
     1081 ggtgtgcggc tccggtaccg agccccacag gaagaagcca accgagcccg caggattggg
     1141 tgtcgtgtcg acgccttccg ccctcagcaa cgccttgacc tgggatgcga accttacggt
     1201 gcacccatcg tccgcgtagt acaggggctt gattccgaag gagtcgcggg ccaggagaag
     1261 gctccggtcg tccgcgttcc agacagcgaa ggcgaacatt cccctgagca tttcgagggc
     1321 tcgtgggccg tgtagggcaa cgagatgcag cagaacctcc gtatcgctct ccgatcggag
     1381 tcgaaccccc gagctatcga gattgcggcg gagctccttg tagttgtaga tctcgccgtt
     1441 gaatgtgatc gtcaagcgcc cgtcggcggt cgtcattggc tgcgcccctg tcgcgctcag
     1501 gtcaatgatg gcgaggcggc ggtgggcgag gcctgctctc tgatcagggg acagccacag
     1561 acccgcccca tcagggcctc ggcgtcccat ggcgtctcgg acgcgcaaca actccgcttc
     1621 atcaaccgga ggcgccgaag cctcatacgc gaacacgccc gcgatcccgc acacggtatg
     1681 cctgctcctt cgggcttgac gctaaggcgg aagtctagac gagcgatgag tccgcgctaa
     1741 tcgcattcga cgagccaccc atcagctcct caatcgcact agccacgagg ctgatcattc
     1801 gcggaggtgc atggtgtcct tgcagcacga tccgcctccc ggcctcagca accgcggccg
     1861 gcagtgcttc ggggctcatc gccaggacaa cacggccctc gcgtgcaagg acctcaacga
     1921 gctccaattg atggtcatcg acgcactcgc caaacgccct tcggcgcggc atgatcactg
     1981 gaactcgtcc cgcatgcagg aggtggagga gcgttccagc accg
//
LOCUS       DASRJQ010000548         2261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_320078, whole genome shotgun sequence.
ACCESSION   DASRJQ010000548 DASRJQ010000000
VERSION     DASRJQ010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320078"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VFB89_12535"
     CDS             complement(<1..241)
                     /locus_tag="VFB89_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28167.1"
                     /translation="MSTTFSLDISVIAELDSVLHSKVARAQTMLARLLEQLRHNSLES
                     RAEVLVMYDETEIRPRGRSWSILSSASRPRKASGIS"
     gene            557..1102
                     /locus_tag="VFB89_12540"
     CDS             557..1102
                     /locus_tag="VFB89_12540"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HZM28168.1"
                     /translation="MTLLDLETNFVLLSATRTRHRDQDYGIVACAEALAAARAGNYGV
                     GAVLVDPNRKIVAQGRNAVFYPYFRSDFHAEMVAMNAFEERHPDVANMRGYTLISSLE
                     PCPMCMTRLLIAGVQTVKFLAYDELAGMVNQKHLLPVAWKRLWERQDYVPADVSPSLQ
                     RFALDVFSLNLEACRQKLWSR"
     gene            complement(1994..2233)
                     /locus_tag="VFB89_12545"
     CDS             complement(1994..2233)
                     /locus_tag="VFB89_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28169.1"
                     /translation="MDRRHGDPTRAVSCLFEPGETGQFGSWDADGFGPRQRSSATATF
                     RAARPGAAAITGNWTGTEGPQSETFEYTITVPNKR"
BASE COUNT          414 a          684 c          709 g          454 t
ORIGIN      
        1 gagaaatacc tgaggccttt cgtggccgcg aggcggacga aaggatgctc cacgatcgtc
       61 cgcgcgggcg gatctcagtc tcgtcgtaca tcaccaaaac ttcggcgcgc gactcgagcg
      121 agttgtgccg caactgttcg agcagccggg cgagcatcgt ctgcgcgcgg gcgaccttac
      181 tgtgcaaaac gctgtcgagc tcggcgatga cggaaatgtc cagagaaaac gtcgtggaca
      241 cgaggggaat ctaacacgag ccgggtcgtt ggtgcgccct cgagagtaca gtcagcgggc
      301 gagggaaccg cctcgagccc tcgtagtggg ccaaccagga cccctcagga tctgctgcgg
      361 gtcgacgccg gtttgaacgg acccctcatt ggctgagcgc ccagtagttg ggcggtggcg
      421 cacagtgatg gggcgacttc tccttgcaca aatctgcttt cacgcccgct caatccaacg
      481 tgagaatcag cacttgaggt gagttgcgct atcgttctcc accatggcat cggaagtgct
      541 ccatgcgaca cgggcgatga cgctgttaga cttggaaacc aattttgttc tcctatcagc
      601 cacgcgtacg cgtcacagag accaggatta cggcattgtg gcctgcgcgg aggcgttagc
      661 ggctgcgcgg gcaggcaact atggtgtcgg cgctgtactg gtcgacccga ataggaaaat
      721 agtggcgcaa ggccgtaacg cagtctttta tccgtacttc cgaagcgatt tccacgccga
      781 aatggtcgca atgaacgcct ttgaggagcg gcatccagat gtggccaaca tgcggggata
      841 cacgctcatc tcctctcttg agccgtgccc gatgtgcatg acacgactcc tcatcgcagg
      901 cgtacaaaca gtgaagtttc tcgcgtacga cgaattagcg gggatggtaa atcaaaaaca
      961 tcttcttcca gttgcatgga agcggctatg ggagaggcaa gattacgttc cggcggacgt
     1021 gtccccgagt ctgcagcgat tcgccctcga cgtcttttcc ctcaaccttg aggcgtgccg
     1081 gcagaaattg tggtcacgtt gagaactctt gagcggccgg ctggagcggt ccctttccag
     1141 ccgccgccgg ccgagtggat cggcgagagg tcgatgcgac gtgcgacgga tcgggctcgc
     1201 ggtcgttgtt gttctcaatt cgcccagaag ccagatggtt agagcgttcc gtcccgcggt
     1261 ctcgcacggt ggtcgttgct ggagtatgct ccggccatag tcatcggctg cggggtgaac
     1321 gatgaggaac gctctcctct tgggcctcag cattctgatg gtcgccttcg tctcgaccgc
     1381 gcccgtgcac gcccgaacag aacatgctgg agcccacggt ttcgacggcc atggcgtact
     1441 ccgctcgccg caccccggta tgcgcgcacc gttccgcggc cgtcacggtt cggtcaggca
     1501 gccattcctt gcttggggcg taggagttgg ttggatggat ccacaggtca ttgtggtggt
     1561 gccgcagttg gtagagccat tgcgagagcc attgagcgct gtcgcgccta tcccagatcc
     1621 gaaattcatg tttccgccca tgccgaatcc accgagcccg gccggttcac atacggtcat
     1681 cgtgcaacga ggctcgaaga tcgaggtggt atcgtttccg accgctcgct gagatgcgca
     1741 gaggtgtggt gacacggaga gctgtcagtt aatttgcaac acccggcgcg gcatacgctc
     1801 agcctgggcg ctgcccatag tttagttctg ctagactccc gccaggcatc ctccacacca
     1861 ggtgcccatg cgggtgatcg ggctcgttct cgcgctcggc ctaatgctgc gccgctcgcc
     1921 cgctgctgag gcagatccca gaatcagcgg aagcaaatcc gcgcgcagga tacggccgca
     1981 gcccagcgcc ggctcagcgc ttgttcggaa ctgtgatggt gtactcgaac gtctcgctct
     2041 gcggcccctc cgtgcccgtc cagtttcccg taatggcggc cgcgccagga cgggcggcgc
     2101 ggaaggtcgc cgttgcgctg ctccgttggc gcgggccgaa cccgtccgcg tcccagctgc
     2161 cgaactggcc ggtctcgccg ggttcaaaga ggcagctgac tgcacgagta ggatctccgt
     2221 ggcggcgatc cattcaccgc gcgcgcaccg agtgactccg t
//
LOCUS       DASRJQ010000549         2421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_729301, whole genome shotgun sequence.
ACCESSION   DASRJQ010000549 DASRJQ010000000
VERSION     DASRJQ010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729301"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VFB89_12550"
     CDS             <1..556
                     /locus_tag="VFB89_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28170.1"
                     /translation="LVVQRQQPGRISWEPLGGISAAIRRSSAAFGSGSRPFMLSKAEL
                     VTATITALESDFCHVALRASVRPIRSAYLGGSATALTLGAGATTALVALSALLPVALI
                     PVPVALGVSYATLRRYRPVPARVLLGLERVLDSLETGTVKPAHQLPRRVPGILDAIIG
                     EVRRSIESAGPPRHSGPRRGTNGS"
     gene            546..1433
                     /locus_tag="VFB89_12555"
     CDS             546..1433
                     /locus_tag="VFB89_12555"
                     /inference="COORDINATES: protein motif:HMM:NF005875.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HZM28171.1"
                     /translation="MAPEIRKVGVLGCGLMGSGIAQAAATSGYTTIVREVDAAALTKG
                     RASIEKSSAKLVDKSKLTAAEREAALARLTFTTELAALSDCDLVIEAVTEDLDLKNRL
                     WRELDGKCPPHSIFASNTSSLTIGAMAAATARADRFVGLHFFNPVPLMPLVEVVRALT
                     TSRETLDRSLEFARSLGKQTVVAKDTPGFLVNLLLIPYLLDAARALERGVGTVADLDL
                     AMRLGCGYPMGPFTLLDFVGIDTTVRIAEIMFDEYRESRYAPPPLLRRMTLAGLHGRK
                     SGKGFYDYSVDPPVPLELA"
     gene            complement(1448..2077)
                     /locus_tag="VFB89_12560"
     CDS             complement(1448..2077)
                     /locus_tag="VFB89_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28172.1"
                     /translation="MGRRRIWSRRRISMGMVGVIIMVMIGGFFALMVMRLARAMDVPF
                     SPIFQLLSHGRVGYAGGVLEHEFAAVMPRVGQETEAHEREQQAGDPLGLAHLAIAPQT
                     PEQHHGKDPERIKGEVFVLLHQLVNGVAMIDDAFDLKAGEHRGTCTERPDQQQDEAGS
                     AESIVLTRHSNQIENHRHRKNCDGQCHEHRMDTGPVVTEHQRSGKGREP"
     gene            2146..>2421
                     /locus_tag="VFB89_12565"
     CDS             2146..>2421
                     /locus_tag="VFB89_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28173.1"
                     /translation="MRGWPTVGTAVLAGGCFLSTTRPAYSPMLGTPSIEIRLGPDTAT
                     QRLAQALRDDSIPVSRIEIRDGYLETPWFDASTGAVAKKSPLGPDVVR"
BASE COUNT          405 a          786 c          780 g          450 t
ORIGIN      
        1 gctggtggtg cagcgccagc agcccggccg tatcagttgg gagcctctcg gggggatttc
       61 cgccgcgatt cgccggtcta gtgcagcgtt cggctcaggg tcgcgcccgt tcatgctttc
      121 caaggcggag cttgtcaccg cgacgatcac ggcgctcgag tcggacttct gtcacgtggc
      181 gcttcgtgcc agcgtgcggc cgatccgaag cgcttacctg ggaggaagcg caaccgccct
      241 cacactggga gccggtgcca cgaccgcgct ggtcgcgctg agtgcactcc ttcctgttgc
      301 gctcattccg gtaccggttg cgctcggcgt gagctacgcc actctgcgcc gctatcggcc
      361 ggtacccgct cgggtcttgc tcgggctcga gcgcgtgctc gatagcctgg agacgggaac
      421 agtgaagccg gcgcatcagc tgcctaggcg cgtacccgga attctggatg cgatcattgg
      481 agaagtccgt cgctcgattg agtcggcagg cccgccgcgc cacagtggac ctcgcagggg
      541 aaccaatggc tcctgagatc cgaaaagtcg gcgtgcttgg ttgcggcttg atggggagtg
      601 ggatcgctca ggccgccgca acttccggtt acaccaccat cgtgcgggag gtggacgcgg
      661 ccgctctcac caagggccgg gcatcgatcg aaaagtcgtc agccaagctg gtcgacaaga
      721 gcaagctcac ggcagctgag cgagaggctg cgctggctcg gctgaccttc acgaccgagc
      781 ttgccgcgct cagcgactgc gacctcgtca tcgaggcggt gaccgaggac ctggatctca
      841 agaaccggct ctggcgagag ctggatggca aatgtccacc ccacagcatc tttgcgtcga
      901 acacctcgag cctcaccatc ggcgcgatgg cggcggctac cgcgcgagcg gaccgctttg
      961 tcggcctgca cttcttcaat cccgtgccgc tgatgccgct ggtggaagtg gttcgtgcgc
     1021 tcaccaccag ccgggagact ctggaccgaa gcctggagtt cgcgaggagc ctgggaaagc
     1081 aaacggtggt cgccaaggat acgccgggct ttctggtcaa cctcctcctg attccttacc
     1141 tgctggacgc cgcccgggcg ctcgagcgtg gtgtggggac cgtcgccgat ctcgacctcg
     1201 ccatgcgact cggctgcggc tacccgatgg ggccgtttac gctactcgac ttcgtcggga
     1261 tcgacacgac ggtacggatc gctgagatca tgttcgacga ataccgcgag agtcgctacg
     1321 cgccgcctcc attgctccgt cgcatgacat tggcaggcct gcacggccgg aagagcggaa
     1381 aggggtttta cgactactcg gtcgatccgc cggttccctt ggagctggcc taggcggcgc
     1441 ttaccgttca cggctcacgt ccctttccgg agcgctgatg ctcggttacg actggccccg
     1501 tgtccatgcg atgctcgtgg cattgcccgt cgcaattctt ccgatggcgg tgattttcga
     1561 tctggttgga gtggcgcgta agaacgatgc tctccgccga accagcctcg tcctgctgct
     1621 gatcgggacg ctcggtgcag gtgccgcggt gctcgccggc cttcaagtcg aaggcgtcat
     1681 cgatcatggc aacgccattc acgagctgat ggaggagcac gaacacctcg ccctttatac
     1741 gctcgggatc tttgccgtgg tgctgctctg gcgtctgtgg cgcgatcgca agatgggcca
     1801 ggccgagcgg atcgccagcc tgctgctctc tttcgtgggc ctcggtttcc tggccaacac
     1861 ggggcatcac ggcggcaaac tcgtgttcga gcacgccgcc ggcgtatcca acgcgaccat
     1921 gagacaagag ttggaaaatc gggctgaagg gcacgtccat ggcgcgggcg aggcgcatca
     1981 ccatgagggc gaagaagccg ccgatcatca ccatgatgat gacgccgacc attcccatgg
     2041 agatccggcg ccggctccag attcgacgtc ggcccacacc gactcgtctc gggccgcacc
     2101 gcacgctcac ccgccgggca ccgcgccgca cagggactaa gccacgtgcg cggatggccg
     2161 acagtcggaa ctgcggtact cgcaggcgga tgctttctct cgacgacccg ccccgcgtac
     2221 tcaccgatgc tcggcacgcc ctcgatagag attcggctgg gacccgacac cgcaacccaa
     2281 cggcttgctc aagcgcttcg ggatgattcg atcccggtat cccggatcga gattcgcgac
     2341 gggtatctcg agacgccctg gttcgacgct tcgaccggtg cagtagcgaa aaagagtccc
     2401 ctgggcccgg acgttgtccg c
//
LOCUS       DASRJQ010000550         6753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_4156132, whole genome shotgun sequence.
ACCESSION   DASRJQ010000550 DASRJQ010000000
VERSION     DASRJQ010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6753
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4156132"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1697
                     /locus_tag="VFB89_12570"
     CDS             <1..1697
                     /locus_tag="VFB89_12570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methanol/ethanol family PQQ-dependent
                     dehydrogenase"
                     /protein_id="HZM28174.1"
                     /translation="GEVHMTGIDYGLQRFSPLKQITTSNVAELRPVWSFSTGTLRGHE
                     GNPLVIGNVMYVHTSFPNIVYALDLSKAGAPQIWKYVPNQSPDAIPIACCDLVNRGTA
                     YHPSGKIFIQLLQGELLALDAKTGKELWKAKNADYKQGSTMTNAPIVIKDLVIAGISG
                     GEFGVRGRVTAYNVNDGKEVWRGYSTGPDSEVLISGDANANYPSHKGKDLGVSTWQGD
                     EWKRGGGTTWGWYSYDPELDLFYYSVGNPGTWNPDQRPGDNKWSMTIFARKPADGKVV
                     WAYQMTPHDEWDYDGVNENVLFENGGKKLLAHFDRNGIAYTIDRTNGKVIVANAYGPI
                     NWAVNKGTGTPVEGVVATGVPKKDPKYGTTSKKNTEGICPAAIGFKDQQPSAYSPMTG
                     LFYVPANHICMDYEGVEVKYSAGQPYVGAIVRMFPGPGGHRGRFIAWDPMSGTVKWEI
                     KENLAAYGGALTTAGGLVFYGTMEGWLKAVDAKSGKVLWQFKTPSGIIGNPMTYLGPD
                     KKQYVAVYSGIGGWAGIGVAAGIGAEDPTAGLGALGAFGDAGQFSTQGGVLTVFSL"
     gene            1860..2762
                     /locus_tag="VFB89_12575"
     CDS             1860..2762
                     /locus_tag="VFB89_12575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated putative
                     ABC transporter substrate-binding protein"
                     /protein_id="HZM28175.1"
                     /translation="MLTLRPLSCSTALALLLAGAPSIAVAQRPGPAQPGILRVCTDPN
                     NLPVSDSMNQGYENKIAQLIADTWHSKLEYVYWRQGFGYFNRGLNGLYCDVEISAPAG
                     IDIVATTRPYFRSAYAIVYRKDSGLNITSLDDPALKRLKIGVNMVGSDGDAGPPAAAL
                     KSHGVVGNLVGFPTFFSEINRPEDIIKAVSDKKVDLAIVWGPTAGYFAAKSPVPLVLN
                     PILADSATGIPFSYSIAMAVARRNKVLRDSLQTFLDQNRGNVEEILKSFGVPLFPVAD
                     SSRSATSPSKSDSAGGIRSTPRGR"
     gene            2885..3346
                     /locus_tag="VFB89_12580"
     CDS             2885..3346
                     /locus_tag="VFB89_12580"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZM28176.1"
                     /translation="MSSSSTAWYAWLLSAVLGGLGLASGATPARAQGQHDAYHEAPRD
                     TISQEAYQGWKQFELNCSRCHGEYGVGTSFAPALIVSLKDGGTIPNKEVFIQTVCAGR
                     PDKGMPSWCALGLEMEKIQNIYAYLKGRADGKIHIGRPAAKAGPEPGKSSS"
     gene            3426..4238
                     /locus_tag="VFB89_12585"
     CDS             3426..4238
                     /locus_tag="VFB89_12585"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HZM28177.1"
                     /translation="MHPLLAASMIGLLLSPRLLAQEAAAHSRMTAAEVKAKLAAATEQ
                     SPPDLSGADLSGLELDGVDFRRAKLSGVQLVKTRLAKANLFTCDLTDAVLTGADLREA
                     NLDGTVLRRANLHEADLEGASLFATIIERADLSGANLRKARIIGYLKNANLAKAVLRE
                     ANIGADPGNQSMGVMRATFVGADLSGADLSSANLYKADFSFANLTGARLAGADLGTTD
                     LVQTDFSGADITGANFARADINGAIFRGVVGRAQAKGFDQARNRDKAIFDAQ"
     gene            4228..5355
                     /locus_tag="VFB89_12590"
     CDS             4228..5355
                     /locus_tag="VFB89_12590"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HZM28178.1"
                     /translation="MRSRIFLAAALFATAACSSGADRKPDTQPSADSASSAAAPKSSF
                     SEAAASGSELAYVTNEDSRNLSIVDAGTDRVVATIEVGTRPRGVHATPDGRTILVALS
                     GSPKCPPTMPDAECEKLQADKSKDGIAVVDAATRKVTRVLPGGSDPENFDISADATRL
                     FVSNEDAGVASVVDIASGKIVSSVRVGREPEGVRVQPDGKAVWVTGETDHNVTILDAA
                     TGKRLSAVSVGKRPRGIAFTPDGKRAYVTCEVDGTVWVIDAHARKVLKTLTMPTGSKP
                     MGVAVSPDGSRVYVSTGRGGTVQAIDTGTNEIVGAVEVGKRPWGIALTADGRKLFTAN
                     GPSNDVAVVDTQTLKVIARIPVGEVPWGVAVAPAPRATPAS"
     gene            5307..5933
                     /locus_tag="VFB89_12595"
     CDS             5307..5933
                     /locus_tag="VFB89_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28179.1"
                     /translation="MGSSGGPGTESNAGKLIGQSRPGRHTCPKKGAWAWVAAALLAHT
                     AAAPVAPAQQLHYESPSRVTATRFEVDTEAHQPLRTLWENSNAAGAERVACIGGDITD
                     GVGHISRVLLLEPGDADSLGISASASIDRCAPPRWFGTAHTHIALYDGQHPYPNFSGA
                     DRGVMMLWWRRWTADGVFCVLYSSREAHCELQSTSASLIAGPGTRVSY"
     gene            complement(5947..6480)
                     /locus_tag="VFB89_12600"
     CDS             complement(5947..6480)
                     /locus_tag="VFB89_12600"
                     /inference="COORDINATES: protein motif:HMM:NF016089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HZM28180.1"
                     /translation="MPDRPVDMTRPSEWLAVLRILVGLYFVKSLVTKMTVVLAGGVLP
                     VPTVSERWLGVMPKIVAKQASENPIAFYKDFLEHTVLTHAGLFAHLTAWGETVAGLGL
                     TLGFCTGLAAIVALVLVTNYGLATQWMSSGQQGFHLVLFALMVAFFFARAGRRWGLDA
                     LIVARRPGSFLTRRPIS"
     gene            complement(6498..>6753)
                     /locus_tag="VFB89_12605"
     CDS             complement(6498..>6753)
                     /locus_tag="VFB89_12605"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZM28181.1"
                     /translation="PYHRVQDGVVYPAVLLATAESDTRVDPMHARKMTARLQAATSSA
                     HPVLLRLESKAGHGAGKPIAKVLDELTDTWSFVFQELAVD"
BASE COUNT         1283 a         2135 c         2179 g         1156 t
ORIGIN      
        1 cgggcgaggt gcacatgaca ggtattgatt acggcttgca gcggttcagc ccgctcaagc
       61 agatcacgac ctcgaatgtt gccgagctcc ggccggtctg gtcgttctcg accgggacgc
      121 tgcggggcca tgagggcaac cccctcgtga tcggcaacgt gatgtacgtc catacgtcct
      181 tcccgaacat cgtgtacgcg ctcgatctgt ccaaggccgg cgcgccgcag atctggaagt
      241 acgtacccaa ccagtcgcct gacgcgatcc ccatcgcctg ctgcgacctg gtcaaccggg
      301 gcaccgcgta tcaccccagc ggcaagattt tcatccagtt gctgcagggc gagctgctcg
      361 cgctcgatgc caagaccggc aaagagctgt ggaaggccaa gaacgccgac tacaagcagg
      421 gcagcaccat gaccaacgcc ccgatcgtca tcaaggacct ggtcatcgcc ggtatttccg
      481 gtggtgagtt cggcgtccgc ggccgagtga cggcgtacaa cgtgaacgac ggcaaggaag
      541 tctggcgcgg ctacagcacg ggtccggaca gcgaagtcct catctcgggc gacgccaacg
      601 ccaactatcc gtcccacaag ggcaaggatc tcggcgtgtc gacctggcag ggcgatgagt
      661 ggaagcgggg cggcggcacc acctggggct ggtactccta cgaccccgag ttggacctct
      721 tctactacag tgtaggcaac ccggggacct ggaaccccga tcagcgcccg ggcgacaaca
      781 agtggtcgat gaccatcttc gcccgcaagc cggccgacgg taaggtcgtt tgggcctacc
      841 agatgacgcc gcacgatgag tgggactacg acggcgtaaa cgagaacgtc ctgttcgaga
      901 acggcggcaa gaagctgctg gctcacttcg atcgtaacgg gattgcgtac acgatcgatc
      961 ggaccaacgg caaggtcatc gtcgccaacg cctacggccc gatcaactgg gccgtgaaca
     1021 agggcaccgg caccccagtc gagggtgtgg ttgccaccgg cgtgccgaag aaggacccga
     1081 agtacggcac gacctcgaag aagaacaccg agggcatctg ccccgcggcg atcggcttca
     1141 aggatcagca gccgtcggcg tactcgccga tgaccggcct gttctacgtg ccggccaacc
     1201 acatctgcat ggactacgag ggcgtcgagg tcaagtactc ggccggtcag ccctacgtgg
     1261 gcgccatcgt gcggatgttc ccgggcccgg gcggccaccg tggtcgcttc atcgcctggg
     1321 atccgatgag tggcaccgtg aagtgggaaa tcaaggagaa ccttgcggcg tacggcggtg
     1381 cccttacgac cgccggtggc ctggtgttct acgggacgat ggaaggctgg ctcaaggcgg
     1441 tcgacgccaa gagcggcaag gtcctctggc agttcaagac cccctcgggc attatcggca
     1501 acccgatgac ctacctcggc ccggataaga agcagtacgt ggccgtgtac tcgggcatcg
     1561 gtggctgggc cggcatcggc gtggcagccg gtatcggcgc cgaggatccc accgcgggtc
     1621 tgggcgcgct cggcgcgttc ggcgacgcgg gtcagttctc cacccagggt ggcgtgctga
     1681 cggtcttctc gctctaagct ttaccccgcg agcccggggg agaccccggg tccgcggact
     1741 gatgcaggcc agggggcgtg gatcgagcgg gggcacgcgg caagcctgtg tgcttcctca
     1801 ccatccgacg cccccttgcc atcttgtgcc ttcggcgctc cgcccgcata taatccagta
     1861 tgctgaccct tcggcccctc agctgctcga cggcgctcgc tctcctgctg gcaggtgctc
     1921 ccagcatcgc tgtggcacag cggcccggcc cggctcagcc cggcatcctg cgggtgtgca
     1981 cggatccgaa caacctcccg gtctcggatt cgatgaacca gggctacgag aacaagatcg
     2041 cccagctcat cgcggacact tggcattcca agctcgaata cgtgtactgg cggcaggggt
     2101 tcggatattt caaccggggg ctcaacggcc tctactgcga tgtggagatc tctgcccccg
     2161 cggggatcga tatcgtcgcg acgacgcgtc cctactttcg ctccgcttat gccatcgtct
     2221 accgcaaaga cagcgggctc aacatcacgt cactggacga ccccgcgctc aagcgtctga
     2281 agatcggcgt caacatggtg ggttcggacg gcgacgccgg gccacccgct gctgcgctca
     2341 agagtcacgg ggtggtcggg aatctggtcg gctttccgac cttcttcagc gagatcaacc
     2401 gcccagagga tatcatcaag gcggtgagtg acaagaaggt agacctcgcg atcgtgtggg
     2461 gaccgactgc gggatacttt gcggcgaaat cgccggttcc cctggtactg aacccgattc
     2521 tggccgactc tgccaccggc attccgtttt cgtactcgat tgcgatggcg gtggctcggc
     2581 gcaataaggt cctgcgcgac agcctccaga ccttcctcga ccagaaccgc ggcaacgtcg
     2641 aagagattct gaagtcattt ggggttcctc tgtttccggt cgccgactcc agtcggtcgg
     2701 cgacgtctcc ctccaagagc gattccgctg gggggattcg atcgacgcct cgaggccggt
     2761 agtcagcccc ggggagcaca acctgccgtg tgagagcgcc ggtccctcgc ggcgtgcgct
     2821 gcgcctcgcc acccgctagg gacgattgtt tggtacccta ctgcgtgaaa ggaccgttcg
     2881 tcagatgagc tcttcctcga ccgcctggta tgcctggctg ctgagcgcgg tgctcggcgg
     2941 actgggactc gcttccggcg caacgccggc gcgggctcag gggcagcacg atgcctacca
     3001 cgaggcgccg cgcgacacta tctctcagga ggcgtatcag ggttggaagc agttcgagct
     3061 gaactgctcc cgctgtcacg gtgagtatgg cgtcgggacg tcgttcgcgc cggcactcat
     3121 cgtctcgctg aaggacggcg gcacgatccc gaacaaggaa gtctttatcc agacggtctg
     3181 tgcaggacgg cccgacaagg gaatgccttc gtggtgcgcc cttgggctgg aaatggaaaa
     3241 gatccagaat atctatgcgt acttgaaagg gcgggcggat ggaaagatcc acatcggccg
     3301 acctgccgcg aaggccggac cggagccggg caagtcgagc agctgaagcc cggctgggtt
     3361 cgacgggacc gcgcagcgcc ggcggtcccg tcgccatttt cacttggaga tcccgccacg
     3421 agcccatgca tccactctta gcggcatcga tgatcgggct gttgctctca ccgcgcttgc
     3481 tggctcagga ggcggcggct cactcccgca tgacagcagc ggaggtgaaa gccaagctcg
     3541 cggccgccac cgagcagagc cctcccgacc tgagtggggc cgatctcagt ggtctcgagc
     3601 tcgacggtgt cgattttcgc cgcgccaagc tcagcggggt ccagctggtc aaaactcggc
     3661 tcgccaaggc aaatctgttc acctgcgatc tcaccgacgc cgtgctgaca ggcgccgatc
     3721 tgcgtgaagc aaacctcgac ggcaccgtgc tccgtcgggc caatctgcac gaagccgatc
     3781 tcgagggggc aagcctgttt gcgactatca tcgaacgtgc agacctgagc ggcgccaatc
     3841 tgcgcaaggc tcggatcatc gggtacctca agaacgccaa cctggcgaag gccgtgttgc
     3901 gcgaggcgaa tatcggggct gatcccggca accagtcgat gggcgtgatg cgagcgacgt
     3961 tcgtaggcgc ggacttgagc ggcgccgatc tgtccagcgc caacctgtac aaggcggact
     4021 tctcattcgc caacctgacc ggcgccagac tggcgggcgc cgatctcggc acaaccgacc
     4081 tggtccagac ggatttctcc ggcgcagata ttacgggcgc caatttcgcg agggctgata
     4141 tcaacggggc catcttccgc ggcgtcgtag ggcgagccca agccaaagga ttcgatcagg
     4201 cccgcaatcg tgacaaggca atctttgatg cgcagtagaa tttttctggc ggccgcattg
     4261 tttgctaccg ccgcatgtag ctccggcgcc gatcgcaagc cggacacgca gccctctgcg
     4321 gattcggcct cttcggcggc ggcgccaaag agctcgtttt ccgaagcggc agcttccggc
     4381 agcgagctgg cgtatgtcac caacgaagac tcacgcaacc tgagcatcgt ggatgccggc
     4441 accgatcgtg ttgtcgctac gatcgaggtg gggactcggc cgcgaggcgt tcacgcgacg
     4501 cctgatgggc gcaccatctt ggtcgcgctc agtgggtcgc ccaagtgtcc gcccaccatg
     4561 cccgatgcgg aatgtgagaa gcttcaggcc gacaagagca aggacggcat cgcggtggtc
     4621 gacgccgcga ctcggaaggt cacacgcgtg ctgcccgggg gatccgatcc ggagaacttc
     4681 gacatctccg cggatgctac tcgcctgttc gtgtccaacg aggacgccgg cgttgcgtcg
     4741 gtggtcgata tcgcctcggg aaagatcgtc tcgagcgttc gcgtgggacg cgagcccgaa
     4801 ggcgttcggg tgcagcccga tggaaaggca gtctgggtga caggagaaac cgaccacaac
     4861 gtcaccattc tggatgcggc gaccggcaag cgactcagcg cggtctcagt aggcaagcga
     4921 ccccgcggca tcgcgttcac acccgatggg aagcgagctt acgtcacctg cgaggtggat
     4981 ggcacggtct gggtcatcga tgcacacgca aggaaggtct tgaagacact cacgatgccg
     5041 accggttcga agccgatggg cgttgcggtc tcacctgacg ggagccgggt ctacgtgtcc
     5101 accggacggg gcggcaccgt tcaggccatc gatacaggca ccaatgaaat tgtgggagct
     5161 gtggaagttg gaaaacgccc ctggggcatt gcgctgacgg cggacggccg caaactcttc
     5221 actgccaacg gaccctcgaa cgacgtcgca gtcgtggata cgcaaacgct caaggtgatc
     5281 gcccgtattc cggtaggaga agttccatgg ggagtagcgg tggccccggc accgagagca
     5341 acgccggcaa gctgatcggt cagtctcgac ctggccgcca tacctgcccg aagaagggcg
     5401 cctgggcctg ggtggccgcg gcgttactgg ctcacaccgc cgccgcacca gtggctccgg
     5461 cgcagcagct gcactatgaa agtccctctc gggtgaccgc aacgcggttc gaggtcgaca
     5521 ccgaagccca ccaaccgctg cggacactct gggagaacag caacgctgcg ggagccgagc
     5581 gggtggcctg tatcggaggt gacataacag acggggtcgg tcacatcagc cgcgtgctgc
     5641 tgctcgaacc gggagacgcg gattcgctcg gcatctccgc gtcggcgtcg atcgaccgct
     5701 gcgcgccccc ccgatggttc gggacggcgc acacccatat cgcgctgtat gatggccagc
     5761 acccctatcc gaacttttcc ggggccgacc ggggggtcat gatgttgtgg tggcggcgct
     5821 ggactgccga cggagtcttc tgcgtgttgt acagctcgag ggaagcccac tgcgagctgc
     5881 agagcacgtc tgccagcctc attgctgggc ccggtacgag ggtctcctac tagcgaacga
     5941 gtcctgctag gagatcggcc ggcgggtgag aaaactcccc ggtcggcgcg caacgatcag
     6001 tgcatccagc ccccaccgac gcccggcccg ggcaaagaag aacgccacca tcaaagcgaa
     6061 caggaccaga tgaaatcctt gctgcccgga cgacatccat tgggtggcca ggccgtagtt
     6121 ggtgaccaga accaaagcca ctatagccgc gaggccggtg cagaacccga gcgtcaggcc
     6181 gagaccggca acggtttcgc cccaggcggt gagatgcgcg aaaaggccgg cgtgggtcag
     6241 gacggtatgc tcgaggaagt ctttgtagaa cgcgatcggg ttttccgacg cttgtttggc
     6301 gacgatcttg ggcatcaccc cgagccaccg ttccgaaacc gtgggcaccg gtaacacccc
     6361 accggcaagc acgaccgtca tcttggtcac gagcgacttg acgaagtaga gtcccacaag
     6421 gatccgcaga acagcgagcc attcggacgg tcgcgtcata tcgactgggc gatccggcat
     6481 cgcagactcc tcgagaatca gtctaccgcg agttcctgga acacgaagct ccaggtatcg
     6541 gtgagctcat ccagcacctt cgctatcggc ttgccggcgc cgtggccggc cttggactcg
     6601 agcctgagaa gaaccggatg agcagagctg gtcgctgcct gtaatcgcgc cgtcatcttg
     6661 cgcgcgtgca tcggatcgac ccgtgtatcg ctctcagccg tcgcgaggag aacggcggga
     6721 taaactactc cgtcctgcac ccgatggtag ggg
//
LOCUS       DASRJQ010000551         4872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2465098, whole genome shotgun sequence.
ACCESSION   DASRJQ010000551 DASRJQ010000000
VERSION     DASRJQ010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4872
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2465098"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="VFB89_12610"
     CDS             complement(<1..770)
                     /locus_tag="VFB89_12610"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HZM28182.1"
                     /translation="MGSIVSWGAVLLHFRRLEALNPVPDARRFRTAIIGAILLAAACG
                     GPTEPGLVDADLSADWPTGSPSSQAIDPTAIQNAITHAGTLPRLLSLLVVRNGVLVVE
                     EYFNGNFADSLNDGRSVTKSVMSTLVGLALHQGLLPSLSERLDTHLPPAYINGLLAGT
                     RAISFRDLLTMSGGFEWHEADGTLPASTEYNAWVLSPDPVAWLLARPLVAAPGTRFNY
                     NTAGTHLMSVMLTSALGKPLADFARQELFPSLGIQHQL"
     gene            909..1397
                     /locus_tag="VFB89_12615"
     CDS             909..1397
                     /locus_tag="VFB89_12615"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZM28183.1"
                     /translation="MHPPWMLADFLERLRGVLPRLDALLLGVDDPSARLQIEGKWSIA
                     QNIGHLSDVEELWQERLEDLKNGRKTYTPAVGSRFQELANRHQERSVAATLGEFKARR
                     LRLVDALAQASPELQRSSAFHERLQVPMRLVDCAQFYAEHDDHHLIRIRRLRILVGVD
                     AG"
     gene            1394..1900
                     /locus_tag="VFB89_12620"
     CDS             1394..1900
                     /locus_tag="VFB89_12620"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZM28184.1"
                     /translation="MNSIPAILDALRRAPDIVVPLVREVPPAVLKLRPAPRRWSAHEH
                     ACHLAHVHGLFFDRLEHVLTAPAPVIRPYLPGDQDADDLLLRMDLDTALDQFVADRKR
                     LVTRLETLSSADWARRAEHAEYRTYSVFIMFRHLALHDFLHAYRIEELLLRPEWPTYS
                     DARWPDER"
     gene            1988..2992
                     /locus_tag="VFB89_12625"
     CDS             1988..2992
                     /locus_tag="VFB89_12625"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZM28185.1"
                     /translation="MGPVIRFLTVALALLPAGCTLSSERPRTAALDSLPRWEGFVLTP
                     DSVRLFTRMVGAGRDTIIVLHGGPGLTLDYLAADLEPLAARHTLLFYDQRGAGRSTLV
                     SDSAGLSAQRFVDDLEAIRAHFGLSKPVLLGHSWGVGLAALYLQRYPRTTGQLILVGG
                     VPLRLTNLVAGFEAMDRARDSSELAAMDRWMAARRTNPEDAAACHNYYRLWFRPFFAD
                     TSALSRSRGDFCAGTLESRRNKMEGVDRFTLASLGNWDWRAAMATVPLRSLIIHGTVD
                     PIPLATAQEWASILPNARLLALPNVGHFPYLEAPDQFFAAIDTFLAGTWPAEAKSARP
                     "
     gene            3074..>4872
                     /locus_tag="VFB89_12630"
     CDS             3074..>4872
                     /locus_tag="VFB89_12630"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM28186.1"
                     /translation="MSVPELPAHLEPLTAALAGRYRLERELGEGGMATVYLAHDLKHN
                     RRVALKVLKPELAAVLGAQRFLQEIQVTANLQHPHILPLYDSGSADGALFYVMPLVQG
                     ESLRDRLDREKLLSVDEAIRITRQVAGALDFAHRQGIVHRDIKPGNILLHDGEALLAD
                     FGIALALTEAAGGRLTGTGLSIGTVQYMSPEQAAGERDLDGRSDIYSLGAVTYEMLAG
                     EPPVTGPSVQAMIGKLMTERPTSLRVVRDVVPPPLDDAVMRALAKMPTDRFSSAREFA
                     DALTAPVAPVRRSRTIVLVTLGVLGVVGAYAVAGSGLFRTSTAGPIDRAAPALIRSIA
                     VLPLDNYSADSTQDYFAEGITDELTAALASISQLRVTSRGSAMQFRGKNRPPTPQIGK
                     TLNVDAIVEGSVSRSGDRVRITAQLIDARADKHLWSKSFERNSSDVLALQGELASAIA
                     AEINVRLTESEQSRLATAPSVNVAGHDAYLRGRYFFNRPSDENLQKAIVQFEAAVKLS
                     PAFAPAYSGLSDAYLWAGYNEGFLTASAARPSALAAAKKAVQLDSTSAEAHASLATFK
                     LFYEFDWAGCEREFRRAILLNPNYAFAHDQFALA"
BASE COUNT          837 a         1581 c         1587 g          867 t
ORIGIN      
        1 cagagctggt gttggatgcc gagagacggg aacagctcct ggcgtgcgaa atcagcgagt
       61 ggtttgccca gggcgctggt gagcatcacc gacatgaggt gggtgccggc ggtgttgtag
      121 ttgaaccggg tgcccggcgc cgcgacgagg ggccgcgcca gaagccaggc gaccggatcg
      181 ggtgagagga cccaggcgtt gtactcggtg ctggcgggga gggttccgtc ggcctcatgc
      241 cactcgaaac cgcccgacat cgtgaggaga tcccggaacg agatcgcgcg ggtgcccgcc
      301 aggagcccgt tgatgtaggc cggcggaagg tgagtgtcga gtcgctcgct caggctgggc
      361 agcaacccct ggtgcagtgc cagtccgacc agggtggaca tgacgctctt ggtgaccgag
      421 cgcccgtcgt tgagactgtc agcgaagttt ccgttgaagt actcctccac cacgagcacg
      481 ccgttccgca ccaccaggag gctcagcagc cgggggaggg tgcccgcgtg ggtgattgca
      541 ttctggatcg ctgtggggtc gatggcttgc gatgaaggtg aaccggtggg ccagtcagcg
      601 ctcaggtcgg cgtcaaccag gcccggttcg gtaggtcccc cgcaggcggc ggcgaggaga
      661 atcgcgccga tgatcgcggt gcggaaccgg cgcgcatcag gtacggggtt cagggcctcg
      721 agcctccgga agtggagcag gaccgcaccc caggaaacta tcgatcccac caagcagagc
      781 aaaggacggc gggtgatcga tggatgggat atcttgatat gatgaatatg actgcaaagg
      841 acggcggcac ggttcaaggt tcttcacagt tcgacagacc tccatgggcg cagcggaagt
      901 tctccttcgt gcacccgcca tggatgctgg cggacttcct cgaacgcctt cgcggggtgc
      961 tgcctcgact tgacgcactt cttctcgggg tagacgaccc cagcgcacga cttcagatcg
     1021 aggggaagtg gtcgattgcc cagaacatcg gccatctctc ggatgtcgag gagctttggc
     1081 aggagcgact ggaggacttg aagaatggaa ggaagacgta caccccagcc gttggctcgc
     1141 gatttcagga gttagcgaac cggcatcaag aacgctccgt ggcggcgacg cttggggagt
     1201 tcaaggcgcg ccggctccgc ctcgtcgacg cgctcgctca ggcctcacct gaactgcaac
     1261 ggtcctcggc ctttcatgaa agacttcaag tgccgatgcg gctcgtcgac tgcgcccagt
     1321 tctacgctga acacgatgac catcacctga ttcggattcg aaggctgagg atcctggttg
     1381 gtgtagacgc tggatgaaca gcattcctgc catccttgac gccttgcggc gtgcccccga
     1441 tatcgtggtg ccgctggtac gcgaggtccc gccggcggtt ctgaaactcc gacccgcacc
     1501 acgacgttgg tcggcgcatg aacacgcgtg ccatctcgcg cacgtgcacg ggctcttctt
     1561 tgatcggctc gagcacgtgc tcaccgcgcc cgcgccagtg attcgaccct acttacccgg
     1621 cgaccaggac gcggacgatc ttctgctccg gatggatctg gataccgcac tcgatcaatt
     1681 cgtggcagac cgaaagcgcc tcgttacccg ccttgagacg ctcagcagcg cagattgggc
     1741 ccgcagggcg gagcacgcgg agtaccggac gtattcggtg ttcatcatgt tccgtcatct
     1801 cgccctgcat gacttcctgc atgcgtatcg catcgaggag cttctgctcc gaccggaatg
     1861 gccaacctac tctgacgccc ggtggcctga cgaacgttga aggggcgtgc cttatccagg
     1921 tcagtatgga tgagatatct tgttgttccg gataagggcg attttcacct cggatggagt
     1981 ccgcatcatg ggacctgtca tccgcttctt gaccgtcgcg ctcgctctgc tgccggccgg
     2041 ctgcactctt tcctccgagc ggcccaggac ggcggcgctt gattccctgc ctcgatggga
     2101 aggcttcgtg ctcaccccgg acagcgtccg tctcttcact cgcatggtcg gcgcggggcg
     2161 cgacacgatc atagtgctac acggtggtcc cggtttgacc ctcgactacc ttgccgcgga
     2221 cctggaaccg ctggccgccc gacacaccct gctcttttat gaccagcgag gtgccggccg
     2281 atccaccctc gtcagcgatt cggccggtct gagcgcccag cggttcgtag acgatctgga
     2341 ggccatacgg gcccatttcg ggctttcaaa gcctgtgctg ctcggccatt cctggggcgt
     2401 tggcctggcc gcgctctacc tgcaacgata cccgcgcacc acgggccaac tgatcctcgt
     2461 tggtggcgtc ccgctgcgcc tcaccaatct ggtcgccggc ttcgaagcga tggatcgcgc
     2521 tcgggacagc agcgagctgg cggcaatgga ccggtggatg gcggcgcgac gtaccaatcc
     2581 ggaggacgcg gccgcctgcc acaactacta ccggctttgg ttccgaccct tcttcgcgga
     2641 tacctccgca ttaagccgaa gccgcggcga cttctgcgcg gggacgctcg agagtcgacg
     2701 gaacaagatg gagggggtgg atcggttcac actggcctct ctcgggaact gggactggcg
     2761 agcagccatg gcgacggtgc ctctgcgtag cttgattatt cacggcacag tggacccaat
     2821 cccgctcgct accgcccagg aatgggcgtc gatcttgccc aacgcacgat tgctggcgct
     2881 tccgaacgtc gggcacttcc cctatctgga agctccggat cagttcttcg ccgcgatcga
     2941 cacttttcta gcagggacct ggccggcgga ggcaaagtcc gctcgacctt aggtggcgga
     3001 tgacgtcaga ccctcgcgtt cggctggccg tgctccgccc aacggggtat cctacggcct
     3061 accgaccaac aggatgagcg tgcccgagct cccggcccac ctcgaaccac tgactgctgc
     3121 acttgcgggc cgctaccgcc tcgagcgaga gctcggcgag ggcggtatgg cgacggtgta
     3181 cctcgctcat gacctgaagc acaaccgacg cgtcgcgctc aaggtgctca agcccgagct
     3241 cgcggcggtg cttggcgccc aacgcttcct ccaggaaatc caggtcaccg ccaatctgca
     3301 gcacccccac atcctgccgc tgtacgactc gggaagcgcg gatggcgcgc tgttctacgt
     3361 gatgccgctg gtgcagggag agtcgctgcg ggaccggctg gaccgggaga aactgctgtc
     3421 ggtcgatgag gccatcagga tcacacgaca agtcgccggt gcgctcgatt tcgcacaccg
     3481 gcagggcatc gtccatcgcg atatcaaacc cgggaacatc ctcctgcacg acggagaggc
     3541 cctgctcgcc gacttcggca tcgccctggc cctgaccgag gcggctggcg ggcgcctgac
     3601 cggcacgggg ctgtcgatcg gcacggtgca gtacatgagc ccggagcagg cggcgggcga
     3661 acgcgatctc gacgggcgca gcgacatcta ttccctcggc gcggtgacct acgagatgct
     3721 cgcgggggag ccgcccgtca ccggcccctc ggtccaggcg atgatcggga agctgatgac
     3781 cgagcgaccc acgtccttgc gggtggtgcg cgacgtcgtg ccgccaccac tcgacgacgc
     3841 cgtgatgcgg gcgctggcga agatgccgac cgaccggttc tcgagcgcgc gcgaattcgc
     3901 cgatgcgctc acggctccgg tggccccggt gaggcggtcc cgcacaatcg tgcttgtcac
     3961 actcggtgtc ctcggtgtcg tcggcgcgta cgcagtcgcg gggagcggtc tcttccggac
     4021 ctccaccgct ggccctatcg accgagcggc gcccgccctg atccggtcga ttgccgtcct
     4081 gccgctcgac aactactcgg cggactctac gcaggactac ttcgccgaag ggataaccga
     4141 tgagctgacg gccgctctgg cgagcatcag ccagctccgg gtcacgtcgc gcggctcggc
     4201 gatgcagttc cggggtaaga accgcccgcc gacgccccag atcgggaaga cgctcaatgt
     4261 ggatgcgatc gtggagggct cggtctcccg atccggcgac cgggtgcgga tcacggcgca
     4321 gttgatcgat gcacgggcgg acaagcatct gtggtccaag agcttcgagc gcaactcgag
     4381 cgacgtgctg gcgctccagg gcgagctcgc gtcggccatc gcggccgaga tcaacgtgcg
     4441 cctgacggag agcgagcagt cccggcttgc caccgcgccc agtgtgaacg ttgccggcca
     4501 cgacgcctac ctccggggac gctatttctt caaccgcccg agcgacgaga acctccagaa
     4561 ggcgattgtc cagttcgaag cggccgtgaa gctgagtccg gccttcgccc cggcctactc
     4621 gggcttgtcg gacgcgtacc tctgggccgg ctacaacgag ggctttctga ccgcctccgc
     4681 ggcgaggccc agcgccctgg cggccgccaa gaaggcggtc cagctggaca gcacctcggc
     4741 tgaagcgcac gcgtcactcg ccaccttcaa gctgttctac gaattcgact gggcaggatg
     4801 cgagcgcgaa ttccggcggg ccatcctgct caatccgaac tacgccttcg cgcacgatca
     4861 gttcgcgctg gc
//
LOCUS       DASRJQ010000552         4663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1920732, whole genome shotgun sequence.
ACCESSION   DASRJQ010000552 DASRJQ010000000
VERSION     DASRJQ010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1920732"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VFB89_12635"
     CDS             <1..550
                     /locus_tag="VFB89_12635"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZM28187.1"
                     /translation="VYRLAADLGSGTDRLPWRGRAAAARGGVLLDHGTHLLYQLLDVS
                     GPPSQVTAWAGRLRHSAYDVEDTAHVLLEFPGRIAKIFLTWAGRARENRIRFIGESGT
                     IEWSAGLLTLERARQTTAFDHTAELRKESYATWFADLFAAFACAMDTGDLEPLNDIAK
                     VADLLSMAYSSLHSGVGLAVPA"
     gene            547..1560
                     /locus_tag="VFB89_12640"
     CDS             547..1560
                     /locus_tag="VFB89_12640"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HZM28188.1"
                     /translation="MSRRFQLALLIAGAALFSLLVARIGPARVLENATAVGWLFVPIL
                     LLYGVAYAANVAAWRLTMGDGERPGFWRTYAMTVSGFSLNFVTPMINAGGEPFRIAAL
                     TRWLGRRRAASSVVLHKMLHSLALLLVWLTALCLALAMLPRTRAVVAPLVGGLAIVSA
                     LAALVLSGHARGGLERLLDLLHRAPLLSRLADRLEPRRPALAEMDQQIADFYQASPRR
                     FWSALALEYLSRSLWMVEYWLICIGVGINTTFLKAFMIGGLSSLIQNALFLVPYELGT
                     KEGSLYALFDLAGLDPRAGVYTAVVSRARDMLWIAAGLGLLALVRHEERTASPVTPPE
                     PAV"
     gene            1557..1994
                     /locus_tag="VFB89_12645"
     CDS             1557..1994
                     /locus_tag="VFB89_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28189.1"
                     /translation="MKIFTHILATTLGVQALDLHGRDAALAYGFGVAVDIDHALKAPF
                     YLRAVGLRDQRGYYWRSSLQEPVAFLWIAPLCLWLGTAVPLLFFAIHLLMDYSVSFEK
                     MPFYPYSGWVTRGWLVNVPDNLKEAVLFVALLVANVALWMARR"
     gene            2027..2668
                     /locus_tag="VFB89_12650"
     CDS             2027..2668
                     /locus_tag="VFB89_12650"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HZM28190.1"
                     /translation="MRLLRERLGLSPGQVTWTAFWLSAAAAVAIADRHVGSGLVLMAL
                     GQILDGLDGAMARQYQLASEEGHRLDTLLDRAAEILIFLAFAIAELVPLGHALLAIAA
                     VLLLTTVCDRSGLDPGMKRFALYFGAWIPYPTLFAAIFGVNLAAYVVGLLIIDCQFQV
                     RMDQLGGDLDTVASRAVAGEKSYQLSATSHQSPRKPPPDSLPAFNPGTPTADS"
     gene            2747..>4663
                     /locus_tag="VFB89_12655"
     CDS             2747..>4663
                     /locus_tag="VFB89_12655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HZM28191.1"
                     /translation="MLDLATAALLLAPLYEFTVRREWLRMPDGVRLAATVWQPTPRDS
                     SERFPVLLELLPYRKDDSFYRRDYPLYSYFVQRGYIMVKVDVRGTGGSEGRRPPHEYS
                     DEELNDAVAMIAQLARMPGASGAVGMWGISWGGFNALQVAMRQPPELKAILALHASDD
                     LFHDDVHYIDGVLHIDPYALEIDHENGLPATPDYPLDSAYFANRFDVEPWIFTYLRQS
                     RDGEFWRRNSLRFHPERLTIPVYLIGGLLDGYRDAVPRLLTSLRGPVKAEIGPWEHDW
                     PDVGIPGPHYEWRARAVQWWDYWLKGRKGQLMKEPRLLLFVRDAHPPNDTITTVPGRW
                     QFVDWPMKGTRVATLHPAAERGLLPKPAAPGTDSLRYVPDAGAAAGDWWGDRTGDMRE
                     DDAASLRYDTAPLDRELTIAGFPRLEATVTASAPLANWTIRLEDVSPTGAVALVTGAA
                     ANGTQLRSRLDPRRVSPGQAQRLALDLHFTTWTFRRGHRIRLAVSNAQFPMLWPTPFS
                     MVTTLRTGSDAILRLPLIPPPAGAAPTLPPSEPRLEATDAVTLDSAPPAGSRVAHDPT
                     TGLTTYELRDGDSYRIGDRRVDVSEIETWEVQRDRPANAAFVGDEVHRIRMRQRELEL
                     RTRMEIRSSLEHLRV"
BASE COUNT          749 a         1528 c         1466 g          920 t
ORIGIN      
        1 cgtgtaccgt ctcgccgccg atctcggctc cggcaccgac cgactcccct ggagagggcg
       61 ggcggcggcg gcgcggggtg gcgtgctgtt ggaccacgga actcacctgc tctaccaact
      121 gctcgacgtc tccggaccgc ccagccaggt gaccgcatgg gcgggacggc tccgccactc
      181 ggcgtacgac gtggaagaca cagcgcatgt gttgctcgaa tttcccggcc gcattgccaa
      241 gatcttcctc acctgggccg gccgcgcccg ggaaaatcgc attcgcttca tcggcgagag
      301 cgggacgatc gaatggagcg ccgggctgtt gacactcgaa cgggccaggc agaccaccgc
      361 cttcgatcat accgcggagc tgcgcaagga gtcctacgca acgtggttcg ccgatctttt
      421 cgccgccttc gcgtgcgcga tggacaccgg ggatctcgag ccgctgaacg acatcgcgaa
      481 ggtagccgac ctgctgtcca tggcatactc gtccctccac agcggggtcg ggctcgcggt
      541 cccggcatga gtcggcgttt tcagctggct ctgctgattg ccggtgcagc gctcttctct
      601 ttactggtgg ctcggatcgg gccggcccgc gtgctcgaga atgccacggc cgtcggttgg
      661 ctcttcgttc cgatcctgct gctctacggc gttgcctacg ccgccaacgt ggcggcctgg
      721 cggctgacca tgggtgacgg cgagcggccc ggtttctggc ggacctacgc catgacggtg
      781 tccggctttt cactcaattt cgtcacaccc atgatcaatg cggggggaga gcccttccgg
      841 atcgcggccc tcacccggtg gctcggtcgc cgccgggcgg ccagctcggt agtgctgcac
      901 aagatgctgc actcgctcgc gttgctgctg gtctggctca ccgccctatg cctggcgctg
      961 gccatgctgc cgcggaccag ggcggtggtg gcgccactgg tcggagggct cgcgatcgtc
     1021 agcgcccttg ccgcgctggt tctctcgggc cacgcgcggg gcggactgga gcgactgctc
     1081 gatctgcttc accgggcgcc cctcctgtca cgactcgccg atcggctcga gcctcgacgc
     1141 ccggcactcg cggagatgga ccagcagatc gcggatttct atcaggcatc accgaggcgc
     1201 ttctggagcg cgctggcgct cgagtatctg agccgctcac tctggatggt cgagtactgg
     1261 ctcatctgca tcggcgtggg catcaacacc accttcctca aagcgttcat gatcggcgga
     1321 ctgtcgtcgt tgatacagaa cgcactcttt ctagtgccct atgagctggg caccaaggaa
     1381 ggttcgctct acgccctgtt cgatctcgcc ggcctggatc cccgcgcagg cgtgtacacc
     1441 gcagtggtga gtcgcgcgcg cgacatgctc tggatcgccg ccggcctggg actgctcgcg
     1501 ctggtccggc acgaggagcg cactgcctca ccggtgacgc cacctgagcc tgcggtatga
     1561 aaatctttac tcacattctc gcgaccaccc tgggcgttca agctctcgac ctgcacggac
     1621 gggatgccgc gctcgcctac ggattcggag tcgcggtgga cattgatcat gctttgaagg
     1681 cgccgttcta tctgcgggcg gtcgggctgc gagatcagcg aggttactac tggcgctcgt
     1741 cgctgcagga gccggtggcc tttctctgga ttgccccgct gtgcctatgg ctgggaacgg
     1801 ccgtgccgct gctcttcttc gccatccatc tgctcatgga ctactccgtg agcttcgaga
     1861 agatgccctt ttatccctac tccggctggg tgacccgagg ttggttggtc aatgtcccgg
     1921 acaacctgaa agaagcggtg ttgttcgtgg cgctcctggt ggccaacgtc gcgttgtgga
     1981 tggcacggcg atgaaaaagc tggccgacac actagggagg ccgctcgtgc ggctcctcag
     2041 agagcggctc ggcttgtcgc ccgggcaggt cacctggacg gcgttctggc tgagtgcggc
     2101 ggcggcggtg gcgattgcgg accggcacgt cgggtccggg ctcgtcctta tggcgctggg
     2161 ccagatcctg gatgggctcg atggcgccat ggcgcgccaa tatcagctcg cttccgaaga
     2221 gggacatcgc ctcgacacgc tgttggatcg agctgccgag atcctcatct ttcttgcgtt
     2281 cgcaatcgcc gagctggtgc cgctgggcca cgcgcttctc gctattgcag ctgtccttct
     2341 gcttacgact gtatgcgatc ggtccggcct cgatccgggt atgaaacgct ttgccctgta
     2401 tttcggtgcg tggattccct acccaacatt gttcgccgcc atcttcgggg tcaatcttgc
     2461 ggcatatgtg gtaggactgc tgatcatcga ttgccaattc caggttcgca tggatcagtt
     2521 gggcggggat ttggatacgg tcgcatcgcg agcggtggcg ggcgagaaaa gctatcagct
     2581 atcagcgacc agccatcagt caccccgcaa accacccccg gactcattgc cggcgttcaa
     2641 tcctgggaca cctaccgctg atagctgatg gctgatggct gatagctgat agctgatagc
     2701 tgatagctga tagcgcgcag cccactgcgc cagctatgat tcccccgtgc tcgacctcgc
     2761 cacggccgct ctccttctcg ctccactcta cgaattcacc gtccgtcgcg aatggctcag
     2821 gatgccggat ggcgtgcggc tggcggcaac cgtgtggcag ccgacgccgc gcgactcgag
     2881 cgagcgattc cccgtgctgc tcgagctact gccttaccgg aaggatgact cgttttaccg
     2941 gcgtgactat ccgctctaca gctatttcgt ccagcgcggg tacatcatgg tcaaagtcga
     3001 cgtgcgcggg accggcggtt cggaagggcg tcgtcctcca cacgaatatt ccgacgagga
     3061 actgaacgat gcggtcgcga tgatcgctca gctcgcgcgg atgcctggcg ccagcggagc
     3121 ggtggggatg tggggcatct catggggcgg attcaatgcg ctccaggtcg cgatgcggca
     3181 accacctgag ctcaaggcta tcctcgcgct gcatgcgtcg gacgatctct ttcacgacga
     3241 tgtgcactat atcgacggcg tacttcatat cgatccctac gcgctcgaga tcgatcacga
     3301 gaatggcttg ccggctacgc cagactatcc gcttgattcc gcatacttcg ccaaccgatt
     3361 cgacgtcgag ccgtggatct ttacctattt gcgccagagc cgggacggcg agttctggcg
     3421 gcgcaactcg cttcggtttc acccggaacg gctgaccatc cccgtgtacc tgatcggagg
     3481 actgctcgac ggataccgcg acgccgttcc gcgcctattg acgagtctcc gcggtcccgt
     3541 caaagccgag atcggaccgt gggagcacga ttggccggac gtggggatac caggcccgca
     3601 ttacgagtgg cgcgcccgcg cagtgcagtg gtgggactac tggctcaagg gtcgcaaggg
     3661 tcagctcatg aaggagcctc gcctcttgct gttcgttcgc gacgcccacc cgcccaacga
     3721 caccatcacc actgtgccgg gccgctggca gttcgtagac tggccgatga aaggcacgcg
     3781 cgtggccaca ctgcaccccg ccgcggagcg ggggctcctg cccaagcccg ctgccccggg
     3841 aactgattct cttcgctacg tccccgacgc cggcgcagcg gccggggact ggtgggggga
     3901 taggaccggt gacatgcgcg aagatgacgc cgcaagcctg cgctacgaca cggcccccct
     3961 cgatcgggag ttgacgatcg ccggatttcc tagactcgag gccacggtca ccgcctcagc
     4021 accgcttgcc aactggacga ttcgcctgga ggacgtgtcc cccacaggag cagtcgcact
     4081 cgtcaccggc gcggccgcca acggtaccca actccgctct cggctcgatc cacggcgggt
     4141 atcccccgga caagcgcaac ggcttgcgct cgaccttcac ttcacgacgt ggacgttccg
     4201 gcgaggtcac cggattcggc tagcggtcag caatgcccag tttcccatgc tctggcccac
     4261 gccgttctcg atggttacaa cgcttcggac cgggagcgat gcgatcctgc gcctcccact
     4321 tattcctccg cctgccgggg cagctcccac cctgccccct tctgagcctc ggctcgaagc
     4381 gaccgacgca gtcaccctcg attccgcgcc accggcggga agccgagtgg cccacgaccc
     4441 gactacgggc ctcacgacgt atgagcttcg ggatggtgat tcgtatcgaa ttggagaccg
     4501 ccgagtcgac gtgtctgaga tcgagacctg ggaggtgcag cgtgatcggc ctgccaatgc
     4561 agcttttgtc ggagacgagg tgcatcgcat ccggatgcgg caacgcgagc tcgagcttcg
     4621 aacccgcatg gaaatcaggt caagcctcga acatctgcgg gtt
//
LOCUS       DASRJQ010000553         4846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3767074, whole genome shotgun sequence.
ACCESSION   DASRJQ010000553 DASRJQ010000000
VERSION     DASRJQ010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3767074"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(305..1735)
                     /locus_tag="VFB89_12660"
     CDS             complement(305..1735)
                     /locus_tag="VFB89_12660"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZM28192.1"
                     /translation="MSATLTTGDGSRKQIGSEAIEQLRSGLGGALLAPGIPGYDEARS
                     IWNAMIDRRPGLVARCTNAADVAHAVRFAGEHDLLVAVRGGGHNIAGNGMCEGGLTID
                     LSLMRQVQVDANARTARVGGGATLAEVDRETQTAGLATPLGVNSTTGVGGLTLGGGFG
                     WLSRRFGLTIDNLISTEIVTANGEITRASADENPDLFWAIRGGGGNFGVVTSFEFRLH
                     PVGPEVLSGLIVHPLSDARDVLHFYRDFLPKTPDDFVCWFVMRKAPPLPFLPPEWHGK
                     EILALAACYCGKDLAEGERVAAPLRRFGKPIADVVAPHPYAAWQQILDPLLTPGVRNY
                     WKSHDFTEVSDGLIDALIAHAGKLPDPSTEIAFAQLGGAVARVANDATAYGHRDGQFV
                     MNVHGRWEDPAKDAACIGWARDLFQATVPFATGSVYVNFLTQDETDRVRAAYGRNYDR
                     LSKLKGKYDPENLFRHNQNIRPLAYA"
     gene            complement(1840..4530)
                     /locus_tag="VFB89_12665"
     CDS             complement(1840..4530)
                     /locus_tag="VFB89_12665"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZM28193.1"
                     /translation="MRRSPPLWPGAITAVIAGYAIAYLWWERTGLGSETLRDLVGNVG
                     FMPFNLACLVLNALASRNPTLDPAVRRALRLLAFGGLSVLIGNTVSVYYLLALGSNPP
                     VSWADLFYLGDSLLILFALLSFPLTRRTRLERWKFAIDAAMVLLGGGVAIWYYSVRPT
                     WSAEGGSIVVTVLALAYPLVSLLLLLGVTTVLLRGPLDGNRRAFSLLVAGVIVSIIAD
                     LTFDLVLLETGQRSVSWTDAVYLLTYLLLIASAEYYYRHPVPGIPAAAEQRPRTQPVS
                     PLPYLALFTAYGLLLAAALRPWTDPVSGLAVGAVLITAMVVARQVLAVRENVRLLAEN
                     AARQNEIRFRSLVQHSSDVIIVIRPDGAIRFVSPSVLRVLGHEPDSLTDRSLFELLHP
                     DDRDRARTFCREAGQLRGVTSPVEWRFRQTDGALLHAELIATNLLNEPAVRGIVLNTR
                     DVSERKRLEKELLHQAFHDPLTGLANRALFRDRVSHALTLARRHGHSITVLFLDLDDF
                     KKVNDSLGHAEGDRLLVAAAERFRSCARATDTVARLGGDEFAILVEDSPGPDGHSAMV
                     ERLASAMAQPFMLKGTEVQVNASIGVAVTSGGESADDLLRNADVAMYTAKRRGKGRSE
                     TYQAHMYTDIRERLELESALRTAIEGERLELHYQPIVCLRTGVVRGVEALVRWDHPRL
                     GHLLPEQFISLAEETGLIVSLGSWVLREACRQIRQLRADHPDLDLSIAVNISGRQLQE
                     RDIVEATRRALAESAVEPRAVVLEITESILMQQTTAALERLLAFKSLGIQLAIDDFGT
                     GYSSLGYLRRFPIDILKIAKPFVDDVGAGPERSAIARAIVGLSDTLKLRTVAEGVELA
                     EQREALLALGCELGQGHLFAPALPAAVLRELLASPTRAVA"
BASE COUNT          900 a         1572 c         1589 g          785 t
ORIGIN      
        1 gaaccaggtc gtgaacagcc acaaaaggaa gaccaccagt cgtggtgcaa ccagagcgag
       61 cagggcgact aggcagggca taagtaagcg aacgcgtgag cggccggccg gggttggacg
      121 aatgctgccg aggcacgcga actgtgagat cgagacccca ttggggcgac agaagtcgcg
      181 ttgtctggga ccgactcact ctccaatcag ccggtgccga aagaacgcga cttaacgcgc
      241 gcccctccgg cgcccccatc tcaacggctc gcgtgcctcg cccgcgctca acccaggccg
      301 gctgctaggc gtacgcgagc gggcggatat tctggttgtg ccggaacaag ttctccgggt
      361 cgtacttgcc cttgagcttg ctcagccgat cgtagttccg cccgtaggcc gcccgcaccc
      421 ggtcggtttc gtcctgcgtc aggaaattga cgtacacgct ccccgtggcg aacggaaccg
      481 tggcctggaa caggtcgcga gcccacccga tgcatgcggc atctttggcc ggatcttccc
      541 agcggccatg cacattcatg acgaactggc cgtcccgatg cccataagcg gtcgcatcgt
      601 tggcgacgcg agctacagcg ccgcccagtt gagcgaacgc aatctcggtg ctcgggtccg
      661 gcagcttgcc cgcatgcgcg atcagcgcgt cgatgagccc gtcgctgacc tcggtaaaat
      721 cgtgcgactt ccagtagttg cgcacgcccg gcgtgagtag cgggtccagg atctgctgcc
      781 aggccgcata tgggtgcgga gcaaccacgt ctgcgatcgg cttaccgaat ctgcgcagcg
      841 gtgctgccac ccgctcgcct tctgccaggt ccttcccgca gtagcacgca gccaatgcca
      901 gaatctcctt gccgtgccat tcgggcggaa ggaacggcag gggcggtgcc tttctcatca
      961 cgaaccagca cacgaagtcg tccggagtct tgggcaggaa atcgcgatag aaatgcagca
     1021 cgtcccgcgc gtcgctgagc ggatggacga tcagtcccga gagcacctcc ggtcctaccg
     1081 gatgaagccg gaactcgaac gaggtcacga ctccgaagtt tccgccgcca ccgcgaattg
     1141 cccagaacag atcggggttc tcatccgcgc tcgcccgggt gatctctccg ttggctgtca
     1201 cgatctccgt ggaaatcagg ttgtcgatgg tgagaccgaa gcgccggctc aaccagccga
     1261 atccgccgcc gagcgtgagg ccaccgacgc cggtcgtcga gttgacgccg agcggggtgg
     1321 ccagacctgc cgtctgggtt tcccgatcca cctctgccag ggtcgcgcca ccgccgaccc
     1381 gcgccgtgcg ggcattcgcg tctacctgca cctgccgcat caacgagagg tcgatcgtga
     1441 gcccgccctc gcacatcccg ttgcccgcga tgttgtgtcc gccgccgcgc acggccacca
     1501 gcaggtcgtg ctcgcccgcg aagcgcacgg cgtgtgcgac gtcagcggcg ttggtgcatc
     1561 gagcaaccag gccggggcgt cggtcgatca ttgcgttcca gatgcttcgg gcttcgtcgt
     1621 aaccgggtat acccggcgcc agcaacgcgc cgcctagccc cgatcgaagc tgctcgatgg
     1681 cctccgaccc gatctgtttc cgggagccat cgcccgtagt caacgtggca ctcatcgaac
     1741 cccctgtttg tcactatgcc gatgggatga acccctcccc ggacgccatt gtgcatccgc
     1801 ggctcggggt tgtccagtat ggaaaccgaa gtggggaggc taggcgactg cccgggttgg
     1861 actcgcgagg agctcgcgca gcacggctgc cggcaacgcc ggggcgaaga ggtggccctg
     1921 accaagctca cagccgaggg ccagcagtgc ctcacgctgt tcggcgagct cgacaccttc
     1981 cgcgacggtt cggagcttga gcgtatcgct caggcccacg atggcgcggg cgatggcgga
     2041 tcgctccggg ccggctccaa cgtcgtccac aaacggcttg gcgatcttga ggatgtcgat
     2101 cgggaaccgg cgcagatagc ccagcgagga atagccggtc ccgaaatcat cgatggcgag
     2161 ctgaatcccc agcgacttga atgcgagcag ccgctccagc gctgccgtgg tctgctgcat
     2221 gaggatgctt tccgttatct cgagcactac ggccctcggt tcgaccgccg actcggcgag
     2281 agcacggcgg gtagcctcca cgatgtcgcg ctcctgtagt tgtcgcccgg agatgttgac
     2341 cgcgatggaa aggtcgaggt cggggtggtc ggcgcgcagc tgccggatct gtcggcacgc
     2401 ctcgcgcaac acccaactgc cgaggctcac gatcaacccc gtctcttctg ccagtgagat
     2461 gaactgctct ggaaggaggt gcccaaggcg cgggtggtcc cagcgcacca gtgcttcgac
     2521 gccgcggaca actccggttc ggaggcagac gatcggctga tagtgcagct ccagccgctc
     2581 tccttcaatc gcagtgcgca gcgccgactc gagctccaat cgttcgcgga tgtcggtgta
     2641 catgtgggcc tgataggtct ccgagcgccc cttgccccgg cgcttggccg tgtacatcgc
     2701 cacgtccgcg ttccgtagca ggtcatccgc cgactcgccg ccgctggtca cggctacgcc
     2761 gatgctggcg ttcacctgaa cctcggtgcc cttcagcatg aacggctgcg ccatcgccga
     2821 ggcaagccgc tcgaccatcg cgctgtgacc atcgggtccg ggcgagtcct cgaccaggat
     2881 ggcgaactca tcgcctccca gccgggcgac ggtgtccgtc gcgcgggcgc acgagcggaa
     2941 tcgctcggcg gcagccacca gcaggcgatc gccctcggcg tgaccaaggc tgtcgttcac
     3001 cttcttgaag tcatcgaggt cgagaaacag caccgtgatc gagtggccgt ggcgccgcgc
     3061 gagcgtgaga gcgtgactca ctcgatcgcg gaacagggcc cgattggcca ggccggtgag
     3121 cggatcgtgg aatgcctgat ggagcagctc tttttccagt cgcttgcgct cactcacgtc
     3181 gcgcgtgttg agcacgatgc cccgcacggc gggctcgttc agcaggttgg tcgcaatcaa
     3241 ctccgcatgc agcaacgctc cgtccgtctg gcggaaccgc cactccaccg gtgaggtgac
     3301 ccccctcagc tgcccggcct cgcgacagaa ggtgcgtgcg cggtcgcggt cgtccggatg
     3361 gagtagctcg aacagcgatc tgtcggtcag cgaatcgggc tcatgcccga ggacccgcaa
     3421 gaccgacgga ctcacgaagc gaatcgcgcc atccggcctg atcacgatga tcacgtcgga
     3481 ggaatgctgg accagcgatc ggaaccgaat ctcgttctgc cgcgcggcat tttccgccag
     3541 gagacgcacg ttctcgcgga ccgccagcac ctggcgagcc acgaccatcg ctgtgatgag
     3601 aaccgctccg acggccagcc cgctcaccgg atcggtccag ggtcgcaacg ccgcggcgag
     3661 gagaagcccg taggcggtga acaatgcgag atagggcagc ggactcacgg gttgggtgcg
     3721 cggccgttgc tctgccgctg ctgggatgcc cgggaccgga tgacgatagt agtactcggc
     3781 gctcgcaatg agcagcaggt aagtcagcag gtagacggcg tcggtccaac tgacgctgcg
     3841 ctggccggtt tcgagcagca ccaggtcgaa ggtgaggtcg gcgatgatgc tcacgatcac
     3901 accggcaaca agcagggaga aggcgcgccg gtttccgtcc agggggcccc ggagaagaac
     3961 agtggtcaca ccgagcagca gcagaaggct gacgagcgga taggccaggg cgagcactgt
     4021 gacgacgata ctgccaccct cggcgctcca ggtgggacga acgctgtagt accagatcgc
     4081 cactccaccg ccaagcaaga ccatcgcggc gtcgatggca aacttccacc gctcgagccg
     4141 ggttcgccgc gtaagcggga aggacaggag cgcgaagaga atcagcaggc tgtcgccgag
     4201 gtagaacaag tcggcccagg agaccggcgg attggaaccc agggcgagga ggtaatagac
     4261 ggagacggtg ttgccgatga ggacggaaag gccgccaaag gcaagtaacc gaagagcccg
     4321 gcgaaccgcg gggtcgaggg tcggattgcg ggacgccagc gcgttcagaa ccaggcaggc
     4381 gaggttgaac ggcatgaacc ccacgttgcc taccagatcc cgcaacgtct cgctgcctag
     4441 gccagttcgc tcccaccaga gataggcgat cgcgtacccg gcgatcacgg cggtgatcgc
     4501 accgggccaa agtggcggtg aacggcgcac ttcgccgctt cccgccgaac gggtggcatt
     4561 atgcaactgg ggcgtcaacg gcacctatcg gtttttcggg cggggggcga tatggcaatc
     4621 atcaggccgc cttggtagac cacagggacg acggcctccg actgcttcgc ttgtcctcag
     4681 ggagacccgc tatcctacct cgcagaagcg ccactcgcaa ccacttggcc gtgcggcacg
     4741 gtcgggcaag gccgccgacg gtcagcgatg gcgagcgatg gtcagcgatg acgacaggcc
     4801 gaatcagttg gcgattggat gctgattggt aaaggaggcg gacagg
//
LOCUS       DASRJQ010000554         2385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3183136, whole genome shotgun sequence.
ACCESSION   DASRJQ010000554 DASRJQ010000000
VERSION     DASRJQ010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3183136"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VFB89_12670"
     CDS             <1..586
                     /locus_tag="VFB89_12670"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZM28194.1"
                     /translation="ICAPRGNESVYDDLIQLFRAADIGVVPTISYLSFAVRLNQRPSL
                     LDEDKELAPFKPGRENFGWMLELSPEARSEWAREAQHSRETAIKWWRAGVTLGTGTDI
                     WQIPTGVHLELEELVAAGLTPVEAIRAATGSSARILGAANDLGTIEVGKRADLVILDA
                     DPVADIRNTRRIWQVVHDGRIVDRNAILETARRR"
     gene            complement(686..1330)
                     /locus_tag="VFB89_12675"
     CDS             complement(686..1330)
                     /locus_tag="VFB89_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28195.1"
                     /translation="MNKILLGHKYVGNFIDAPVRLGMIFRMDAQQFIPAMHFNDAYPA
                     INLGKWTEAGSKGNIRFSETTSVSFSFGASATTGIGESELGIKFKKAKSVAGYVQDAV
                     VERFGYSNILPQLKQIWTDQGYAKFCKEYVLVFEVVTAASGTLVYSEDRNNEVVLRHK
                     LGVAVKKIADLGSGNFEFVSNSKRTLEIIRAVAHKPLFKAFRLRKDWRPEILGT"
     gene            complement(1340..>2385)
                     /locus_tag="VFB89_12680"
     CDS             complement(1340..>2385)
                     /locus_tag="VFB89_12680"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HZM28196.1"
                     /translation="PGVVSRLEKLGPRLKVIIDNDGAHAKPKSGEAQAAKRLRVTAGK
                     ANVKRQHMGKLQHNKTIVVDGPKVQAVVCGSTNFTWRGFFVQSNNAIVLRGKSAVKPF
                     LAAFNDYWAHDDPGGFGKTASAKLTALGLTGINARVGFSPYAKKNQLLAGIAEDIGKA
                     TSCVFYSLAFLYQTKGPIRNAITKLTKDDKIFLYGISDRKVGGLDLLKPDGNLAPVRP
                     AALEKNVPEPFKSEPTGGGGNRLHHKFVVIDFDKPTARVYMGSYNFSGAADASNGEQL
                     LLIRDRRIAVSYVIEALRIFDHYYFRVVQQEAKDAKTELVLCKPPRAAGEKPWWADDW
                     SNARRCRDRELFS"
BASE COUNT          416 a          779 c          744 g          446 t
ORIGIN      
        1 tatctgcgcg ccacgcggca acgagagtgt ctacgatgat ctgatccagc tgttccgggc
       61 cgccgatatc ggggtcgttc ccaccatctc gtatttgtcg ttcgcggttc ggctgaacca
      121 gcggccgtcc ctgctggacg aagacaagga gctcgcgccg ttcaagccgg ggcgcgagaa
      181 tttcggctgg atgctcgagc tgagccccga ggctcgcagc gagtgggccc gggaggctca
      241 acactcccgc gagacggcca tcaaatggtg gcgcgccggg gtgactctcg gcaccgggac
      301 cgatatctgg cagataccaa ccggcgtgca cctggagctg gaggagctgg tggccgccgg
      361 cctcacaccc gtggaagcga ttcgcgccgc caccggctct tccgcccgca ttctcggcgc
      421 cgccaacgac ctcggcacga tcgaggtggg gaagcgggcc gacctcgtca ttctcgacgc
      481 cgaccctgtc gcggacattc gcaatacccg ccggatctgg caggtcgtgc acgacggccg
      541 catcgtcgat cgaaacgcca ttctcgaaac cgcccgccga cgctgaaacg cgcggcgctc
      601 aaggccagcc tggtattgga gttctgggtg aggatgagct gagccgcgcc cagcagtcac
      661 gcagccagcg gaatgccgat cggcctcatg ttcccaggat ctcgggtctc caatccttgc
      721 gcagccgaaa cgccttgaac agcggtttgt gcgccacggc gcggatgatc tccagggtac
      781 gcttggaatt cgagacgaac tcgaagttgc ccgagccgag gtcggcgatc ttcttcaccg
      841 cgactccgag cttgtgccgc aggacgacct cattgttccg gtcttcggaa taaaccagcg
      901 tgccggacgc ggcggtgacc acctcgaaga ccaggacgta ctccttgcag aatttggcgt
      961 acccctgatc ggtccagatc tgcttgagct gcgggaggat attgctgtac ccgaaccgct
     1021 cgaccaccgc atcctggaca taccccgcca ccgacttggc cttcttgaac ttgatcccca
     1081 gctccgattc gccgatgccg gtcgtggccg atgcgccgaa cgagaacgac acgctggtcg
     1141 tctccgaaaa gcggatgttg cccttcgacc cggcttcggt ccacttcccg agattgatcg
     1201 cgggataggc gtcattgaag tgcatggccg ggatgaactg ctgagcgtcc atccggaaga
     1261 tcatgcccag gcgcaccggc gcatcgatga agttgccgac gtacttgtgg cccagcagga
     1321 tcttgttcat ggcgtcctcc tacgagaaga gctcgcgatc gcggcaccgc cgggcgttgg
     1381 accagtcgtc ggcccaccag ggcttttctc ccgcagcacg aggcggcttg cacaggacca
     1441 gctccgtctt cgcgtccttc gcctcctgct gcaccacccg gaaatagtag tgatcgaaga
     1501 tccggagcgc ctcgatcacg taggagaccg cgatccgccg gtcgcggatg aggagcagct
     1561 gctccccgtt gctggcgtcc gccgcgccgg aaaagttata ggagcccatg tagacccggg
     1621 cggtcggctt gtcgaaatcg atgacgacaa acttgtggtg gaggcgattg ccgccgccgc
     1681 cggtgggctc gctcttgaac ggttccggca cgttcttctc cagcgccgcg ggacgcaccg
     1741 gcgcaagatt gccatcgggc ttcagcaggt cgaggccgcc caccttgcga tccgaaatcc
     1801 cgtagaggaa gatcttgtcg tccttggtga gcttggtgat cgcattccgg ataggaccct
     1861 tggtctgata gagaaacgcc agcgaataga acacacacga ggtcgccttg ccgatatcct
     1921 ccgcgatccc ggccagcagc tgattcttct tggcatacgg agaaaatccc acccgcgcgt
     1981 tgatcccggt gagccccagc gcggtcagct tcgccgaggc ggtcttgccg aagccgcccg
     2041 ggtcgtcgtg ggcccagtag tcgttgaacg ccgcgaggaa cggcttgacc gcgctcttgc
     2101 cgcgcaacac gatggcgttg ttggactgca cgaagaatcc gcgccaggtg aaattggtcg
     2161 agccgcagac caccgcctgc accttgggcc cgtcgaccac gatggtcttg ttgtgctgca
     2221 gcttccccat gtgctgccgc ttcacgttgg ccttgccggc cgtaacccgc aggcgcttgg
     2281 ccgcctgcgc ttcgcctgat ttgggcttgg cgtgggcgcc gtcgttgtcg atgatcacct
     2341 tgagccgcgg gccgagcttc tcgaggcgcg acacgacacc gggct
//
LOCUS       DASRJQ010000555         2358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2945272, whole genome shotgun sequence.
ACCESSION   DASRJQ010000555 DASRJQ010000000
VERSION     DASRJQ010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2945272"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VFB89_12685"
     CDS             <1..598
                     /locus_tag="VFB89_12685"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZM28197.1"
                     /translation="TTTIPIVFASVGDPVGLGLVSSLARPGGNVTGFSAVVPEGFAAK
                     QFELLHEAVPGAARIAVLMNPANAGHRRSLPQTVAAAEKLKVKLQILEAKTLGELESA
                     FEAATRARAGAVHVYGDPLTWFHRARTAELAAKARLPAIYQFRDNVAAGGLMSYGTSN
                     ADLLRRSAGYVDRILKGAKALGLTIPQAVLGRADQVIE"
     gene            602..871
                     /locus_tag="VFB89_12690"
     CDS             602..871
                     /locus_tag="VFB89_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28198.1"
                     /translation="MLDQRSQLLRAALGFAGLPRPSYDRALHALRSWLDSWSGVGHVA
                     LGMHRQGFDLQLTRYDERGWRATFYTTGMEHSLTSATGSGWERTP"
     gene            complement(1570..2049)
                     /locus_tag="VFB89_12695"
     CDS             complement(1570..2049)
                     /locus_tag="VFB89_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28199.1"
                     /translation="MQVQCAKCSQPIALTDIIESNNGHLSHVDCKRPHVLTPEERALV
                     FVYCSGHVVARCPPCDISFRYTELAADILGGSRTNMCPQCRRELTEAVRAHLFRCAML
                     PSEIRLRVQSVREAAQRLVKETQQLSDRSDVLIREAEAALFESQRALRDAMSKRTTT"
BASE COUNT          421 a          736 c          771 g          430 t
ORIGIN      
        1 aacaacgacg atccccattg tcttcgcgag cgtgggcgat ccagtcggat tgggcctcgt
       61 ctccagcctc gcgcgacccg gtggaaacgt caccgggttc tccgcggtag tgccggaggg
      121 attcgcggcg aaacagttcg agctgctcca tgaggcagtt cccggcgcgg ctcgcatagc
      181 cgtcctcatg aaccccgcaa acgcggggca tcggagaagt ctcccgcaga ccgtagcggc
      241 cgccgaaaag ttaaaggtga agttgcagat cctggaggcc aagaccctcg gcgaattgga
      301 gagcgccttc gaggccgcga cccgggcccg cgccggcgcg gttcatgtgt atggagaccc
      361 cctgacttgg tttcaccgcg cacggaccgc ggagctggcg gcaaaggctc gacttccagc
      421 gatctaccag ttcagggata acgtggcggc cggggggctg atgtcctatg ggaccagcaa
      481 cgccgacctt ctgcggcgta gcgctggcta tgtggacagg atcctcaagg gcgccaaggc
      541 gctcgggctg acgatcccgc aggcggtgct agggcgggcg gaccaagtga tcgaatagcg
      601 cgtgctcgac caacgcagcc aacttctgcg agcagcactc gggttcgccg ggctccctcg
      661 cccgtcctac gaccgtgcgc tccatgcgct gcgctcatgg ctcgattcgt ggtccggtgt
      721 cggccacgtc gccctcggca tgcaccgtca gggcttcgac ctacaactga cgcggtacga
      781 cgagcgcggt tggcgggcga cgttctacac gaccgggatg gagcactcgc tgacaagcgc
      841 gaccggcagt ggatgggagc gcacgccgtg atgtgcgacg cggaagccgc tgaagaagag
      901 tgaccaaagt ccgcgtgctg cgatgtttga ggatgggtca cgcccgcgga agaagccggc
      961 tcgccacgga tcgaaagaaa ttcccgggcc tgtttcgccg cgaaaggagt gtgttgcctc
     1021 ggtgcggcga ctatcttgct ctctagccgc gattggctcg cccgtaactt tgaccgctca
     1081 ggcaaaagac aaactcatcg acttcgacgc tagtcgctcc ggctgcggtc gcttgcgcaa
     1141 gagccctctg tcgcgcctct tcggcgcttt ctgtttgaac gcggaacgta caagcgcatt
     1201 tcactacgta ggtgtccatc ggtgccccgt tggtttccat tggccgcctc cctcgccatg
     1261 gggactgcat ggtcagtgcc aaacgacaag cgcctcaaat cagacggatt cccaatcctg
     1321 ttctgccttc ggtgtccctc ccttgacagt cgtgtaattg tgcgcttggt agtttcttct
     1381 cgctcctgac cgcgatcgag cgagcgtcac ccgccaacgg tgcggcgagt gcgcttggac
     1441 gcgacgttct acaccatggg gcatccgaac gctgccgacg agcgcgaccg gcaccgcatg
     1501 ggagcgcacg ccctggcacg caacgcagcg ggcggcgtgg gaggccctca agagagcgga
     1561 ttgcacaggt taggtggttg ttctcttcga catggcgtct cggagagcac gctggctctc
     1621 gaacagcgcg gcctcagcct ctcggatcag gacatcggac ctgtcgctga gttgctgggt
     1681 ctctttcacg aggcgctgcg ccgcctccct caccgactgc acccggagtc gtatttccga
     1741 gggcagcatc gcgcagcgaa agagatgtgc gcgaacggct tcggtgagtt cccgacgaca
     1801 ctgagggcac atgttcgtgc ggcttccgcc caagatgtcg gccgcgagtt ccgtgtagcg
     1861 gaaactgatg tcacagggcg ggcagcgggc gacgacgtgc cccgagcaat agacgaagac
     1921 cagcgctcgc tcttccggtg tgaggacgtg agggcgcttg cagtcaacgt gcgacagatg
     1981 gccattgttc gactcgataa tgtcggtgag cgcaatcggc tgagagcact tcgcgcactg
     2041 cacctgcatg tgacgcctcg cgcttcggtg tcaagcggtg gtcccggccg cgcaccaatg
     2101 ggcgagatgc ccctgcatca ggctgggcgc tcaatggagc gaggcgacca ggacggtatc
     2161 acagtcacgg acgaggtatc tcgggaatca agcgcgattc ttgccggtga tcgactactc
     2221 gcacgtgcaa caggccgctg gccttgatct cctgaagtga cggcgccagt cctcatggcg
     2281 ccagtcctca tgatgacggg cggcgatggc ggcactccga gggaggtcga ggcgtgccgc
     2341 tacgcgcgca gcccattg
//
LOCUS       DASRJQ010000556         2442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2945904, whole genome shotgun sequence.
ACCESSION   DASRJQ010000556 DASRJQ010000000
VERSION     DASRJQ010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2945904"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VFB89_12700"
     CDS             <1..231
                     /locus_tag="VFB89_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28200.1"
                     /translation="LGFAWDRTTDDLDPSWDFGRTGALHLVGWNASARIAERHEATLF
                     LGKRRGGLACTAGTCYQVLPFEGAELRLVSRF"
     gene            320..1021
                     /locus_tag="VFB89_12705"
     CDS             320..1021
                     /locus_tag="VFB89_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28201.1"
                     /translation="MNRLSRFLAMAGISLLAVQSNAAPASKAGVWATDFSIDPAHLTS
                     VGRNPYFVLEPGHYRILRGGEEEITMTVLDETRIVAGVETRVIEEREMVNGQLAEISR
                     NFFAINKLNQDVLYFGEEVDMYKDGKVVSHDGSWVAGVNDAKAGLIMPGKPRKGFRHY
                     QEQAPGVAMDRAEILSTTEKVQTDAGTYEKCLKVVETSPMIRGAEYKYFAPGIGLIKY
                     EKLLLVKQGMKVASQ"
     gene            1701..>2442
                     /locus_tag="VFB89_12710"
     CDS             1701..>2442
                     /locus_tag="VFB89_12710"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZM28202.1"
                     /translation="MIGSLVAACLRFRPLVVAVAAAVIVVGVTRLRDMPMDVFPEILP
                     VTVQVQTEALGLSAAEVEQLITVPIEADLLAGTPWVDVMRSESVPGLSSIELTFKRGT
                     DPMHARQVVQERLTQAHALPNVSKPPHMLQPLSSTNRVMLIGLSSKTQSLIEMSVQAR
                     WTIRPRLMGVPGVANVAIWGQRERQLQVQVDPKRLAEKNISLLQIVKTTGNSLWYSPL
                     SFLEASVAGTGGFIETPNQRLGVRHVLPI"
BASE COUNT          425 a          771 c          822 g          424 t
ORIGIN      
        1 ctgggcttcg cctgggatcg caccaccgac gatctggatc cgtcgtggga cttcggccgc
       61 acgggcgcgc ttcacctggt gggctggaac gcgagcgcgc ggatcgcaga gcgccacgag
      121 gcgacgctgt tcctgggcaa gcgccgcggg ggactcgcgt gcacggccgg cacctgctac
      181 caggtgctgc cgttcgaagg tgcggagctg cggctggtga gtcggttctg agagaacaca
      241 aacccgtggc acttcgcgtt ggatcgaatc tagaatagtg atcgagaaac catgcttgag
      301 gactatggag ggagagacca tgaacagact tagccgattt ctggcaatgg ccggtatcag
      361 tctgctggcc gtgcagtcga acgcggcgcc ggcgagcaag gccggagtct gggccaccga
      421 cttctccatc gacccggcac acctcacgtc ggttgggcga aatccctact tcgttctcga
      481 gcccgggcac taccggatcc tccgcggggg cgaggaggag atcaccatga ccgtcctcga
      541 cgagacccgg atcgtggcgg gcgtggagac ccgggtcatc gaagagcgcg agatggtgaa
      601 cggccagctg gccgagatct cgcggaattt cttcgccatc aacaagctga atcaggacgt
      661 gctgtacttc ggcgaagagg tggacatgta caaggacggc aaggtggtga gccacgacgg
      721 gagctgggtt gccggcgtca acgatgccaa ggccggcctg atcatgccgg gcaagccccg
      781 gaagggtttc cgtcactacc aggagcaggc gccgggcgtc gccatggatc gcgccgagat
      841 cctgagcacg acggaaaagg tccagacgga cgccggcacg tacgagaagt gtctgaaggt
      901 cgtggagacg agccccatga tccggggcgc ggagtacaag tacttcgcgc cgggcatcgg
      961 actgatcaag tacgagaagc tgctgctggt gaagcagggg atgaaggtcg catcgcagta
     1021 ggacgcgacc cggtcgtccg tcgttcccat tgcccgcgac atcgagcgcg ggcggaggca
     1081 ggctccccga cgcgtgacgt cgggcccctc cgcccgcgct cgattcttgt gcccgcggca
     1141 ggcgaggcgc tgcgcctcgg ccccgagtct cgccgctcgc gccagggccg cgcggaccgt
     1201 tcgccccacc ggtcgcgccg cccgcaggat ccagaccgag agatgttccg gggccgcgcc
     1261 gggggtatcc cggcggctcg tcaggtgccg ggcgacctgc ggcgccctcg atcgacggcc
     1321 agactatata aatattagtg gcgatgcacg tcgaatcgca acggcgcccg ggagccgact
     1381 ttcagcaagc gatcgacaca aattcctttt gtgagtttca gtaggcggac tacaatctca
     1441 tttgcaatat cttgttggca tattctgtcg ttaccgcgct gtcacggcgc ggttgctcgg
     1501 gaggcagccc gcagttgggt tgttccgcga accgctgggg atgcggtagt tgggagaggg
     1561 gaatccccgg acggttcgca ggaattgact gcttcccatt cccatggccg gtcgaggtgt
     1621 tggcggctac tcgaccggag ccgcccgggt ggtgacacgg ggcggagacc gcatatgagc
     1681 ccgcttggag gcccctctca atgattggtt cactcgttgc tgcctgcctt cgattcaggc
     1741 ccctcgtagt cgctgttgcg gcggccgtca tcgtggtcgg ggtaacacgt ctcagagaca
     1801 tgccgatgga cgtgttcccc gagatcctcc cggtcacggt ccaggtccag accgaagcgc
     1861 tcgggctctc agccgccgaa gtcgagcagc tgatcaccgt gccgatcgag gccgacctcc
     1921 tggccggcac gccctgggtc gacgtgatgc gctcggagtc cgtgccgggt ctgtcgtcga
     1981 tcgagctgac gttcaagcgc ggcacggatc ccatgcacgc gcggcaggtg gtccaggagc
     2041 ggctgacgca ggcgcacgcc ctgcccaacg tttccaagcc gccccacatg ctccagccgc
     2101 tgtcgtccac gaatcgcgtc atgctgatcg gactgtcttc caagacccag tccctgatcg
     2161 agatgtcggt gcaggcgcgg tggacgatcc ggccacgcct gatgggcgtt cccggcgttg
     2221 ccaacgtggc catctggggc cagcgcgagc gccagctgca ggtgcaggtc gatcccaagc
     2281 gtctggcgga gaagaacatc tcgctcctcc agatcgtcaa gaccaccggc aactcgctct
     2341 ggtattcacc gctgagcttc ctcgaggcgt cggttgccgg caccggcggc ttcatcgaga
     2401 cccccaacca gcgtctcggg gtccgtcacg tgctgcccat ca
//
LOCUS       DASRJQ010000557         4781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_666242, whole genome shotgun sequence.
ACCESSION   DASRJQ010000557 DASRJQ010000000
VERSION     DASRJQ010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666242"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VFB89_12715"
     CDS             complement(<1..525)
                     /locus_tag="VFB89_12715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HZM28203.1"
                     /translation="MAHPTNQGGSVDPGPVTGNPRVSVVVPFWNAKPFIEEAIRSVLD
                     QTFADWELLLVDDGSTDGSTQIALQYVERLPEKLRYFEHEHHQNRGVAASRNLGVRQG
                     RGGYIAFLDADDVWVSNKLARQLAILESERNAAMVCGPSLFWYSWTGKAEDIARDFVK
                     DLRISPAGLIRPPAL"
     gene            complement(616..1593)
                     /locus_tag="VFB89_12720"
     CDS             complement(616..1593)
                     /locus_tag="VFB89_12720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZM28204.1"
                     /translation="MPTVSVLMPVHNADRYVAKAVDSILGQSWGDWELLTIDDGSTDR
                     SRAILERYATRDHRIRLTSRPNTGYTVALNELLTQASGEFVARMDADDVALPERLARQ
                     MEYLRANPAVVCVGTAVHFIDDAGRFLRDGHPGLDNDEIQQRILAGDCVLNHPSVMMR
                     RRAVESVGGYRAEFEPAEDLDLWLRLAEIGRLANLAEPLMNYRQHPASFSERHQMLQL
                     ERSAAAVLDACRRRGIPPRKIELSAWRPVDRRSRMETYLGYGRQGLERGEYGTAWHYG
                     LQAIGQAPWRLGGWGLLARTFGRRLVRGLRRRSPSHDPEQAAREPAVRS"
     gene            1732..2679
                     /locus_tag="VFB89_12725"
     CDS             1732..2679
                     /locus_tag="VFB89_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28205.1"
                     /translation="MTDRANGGQTVSCDLSILVSRIDTSFMLKTIPHLVRACRFPFAR
                     RVLVTDTAPLGRRYARRPGIGTMEDLRACCAQLVQAGIIDVIAPIDYSPGYHRRMYRK
                     HFSGLMRQTHSNGGYPILGSIFAVEESTADYLVHFDSDMLLYQAPGFNWIEEGIRLLQ
                     RHEDLIAVLPRSGPPAADGRLKQQEETGERFEQDSRGLFCFHTFTSRVFLIDRKRFER
                     VLPLRPRMPFPELLRNYFTKRNTMPEWEVMVGYRLPETGLVRADLSTPDAWTIHPNDR
                     GEQFDAVLPNLIARVEAGDFPPDQGGYYDLRLDRWAAAS"
     gene            2694..3839
                     /locus_tag="VFB89_12730"
     CDS             2694..3839
                     /locus_tag="VFB89_12730"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZM28206.1"
                     /translation="MTDTPLVSVVMPCLNEEAAIGACIEKIQRTFAAAGISGEIVVCD
                     NGSTDASVAIAERMGARVVHQPRRGYGNAYLKGFAEARGEYLIMGDADDTYDFSLIPQ
                     FLDGLRNQGFDFVTGSRYLAGGDKHVTGLHRFVGNPALTAILNFLFSTRYTDVYCGFR
                     GFSRAAYDRIRPVSPGMEFNLELAINARLAGLKISEIPIELGPRKGESKLRTFRDGWR
                     SLRMMLLYSPNQLFFAPGGILLGCGLVIHVLVLLGIVKFGGRPAAGVTGVFATILSVV
                     GFQILSLGLHAKTYSWSRRFDLNNRALAAFYDRFKLEEGLLLGAGLVLLGAGILTALV
                     IEWLRGDLLPLPRPEWASFGATLVIIGFSTIFTSLFISAMSINKPAA"
     gene            3848..3997
                     /locus_tag="VFB89_12735"
     CDS             3848..3997
                     /locus_tag="VFB89_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28207.1"
                     /translation="MAQALIRVGRAVYHAVSRKREIVYVPWFWRPIMLAVRLVPERVF
                     KRLHF"
     gene            4037..>4781
                     /gene="rfbA"
                     /locus_tag="VFB89_12740"
     CDS             4037..>4781
                     /gene="rfbA"
                     /locus_tag="VFB89_12740"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HZM28208.1"
                     /translation="MRKGIILAGGSGTRLWPSTRVVSKQLLPIYDKPMIYYPLSTLLL
                     AGLRDILVISTPDDLPRFSELLGDGERLGVSLSYAAQPRPEGLAQAFLIGANFLNGAA
                     TTLILGDNLFFGHGMPEQLQRAGSASTGATIFAYHVRDPERYGVVEFDDAGKALSIDE
                     KPARPRSQYAVVGLYFYDAEVVSLARRLKPSARGELEITDLNRAYLEAGRLTVELLGR
                     GVAWLDTGTHDALLQASHFVSVVEERQGLK"
BASE COUNT          815 a         1503 c         1527 g          936 t
ORIGIN      
        1 aagggcaggg ggccggatca gacctgcggg cgagatgcga agatccttga cgaagtctcg
       61 cgcgatatcc tccgccttgc ccgtccagct ataccagaat agggaaggcc cgcaaaccat
      121 cgccgcattg cgctccgatt ccaggatggc caactgtcgc gcaagcttgt tggagaccca
      181 cacatcatca gcgtcgagaa aggcgatgta cccgccgcgg ccctggcgaa ctccgaggtt
      241 acgcgaggcc gctacgccgc gattctgatg gtgctcgtgc tcgaaatagc ggagcttctc
      301 gggcaatcgc tccacgtatt gcagagcgat ttgggtgctc ccgtcggtgg agccatcatc
      361 gacgagcagg agctcccagt cggcgaaggt ctggtccagg acgctgcgaa ttgcttcttc
      421 aatgaaaggc ttggcgttcc agaatggaac cacgaccgag actcgaggat tccccgttac
      481 aggccctggg tccacgctgc cgccctggtt cgttggatgc gccatctctc ctgtccgggc
      541 cgccgccgac agacgacaac aggagtgtcg cctctgcctg acaacccgcc tgtagccagc
      601 gggggcctat ggatgtcagg agcgaactgc cggctcacgg gccgcttgct ccgggtcgtg
      661 ggacggcgat cgtctgcgca atccccggac cagtcggcgc ccgaacgtgc gggccaagag
      721 cccccagccg ccaaggcgcc agggtgcttg cccgatcgcc tggagcccat agtgccacgc
      781 cgtcccgtac tcgcctcgct cgagcccctg gcgcccgtaa ccgaggtacg tctccattcg
      841 cgagcgccgg tccaccggcc gccacgcgct cagctcgatc ttccggggcg gaatcccgcg
      901 cctgcggcag gcatcaagca ccgcagccgc cgaccgctcg agctggagca tttgatgccg
      961 ctcgctgaac gacgccgggt gctgtcggta gttcatcagc ggttcggcga gattcgcgag
     1021 ccgcccgatt tcggccagcc gcagccagag atccaaatcc tcggccggct cgaattccgc
     1081 gcggtaaccg ccgaccgatt cgaccgcgcg tcggcgcatc atcacgctgg ggtggttcag
     1141 gacgcagtct ccggccaaga ttctctgctg gatctcatcg ttgtcgaggc cggggtgccc
     1201 gtcgcggaga aatcgcccgg catcatcaat gaagtgcacc gcggtgccga cgcagacgac
     1261 ggcagggttc gcccggagat attccatctg gcgagccagc cgctccggca gggccacgtc
     1321 gtcggcatcc atccgggcaa cgaactcccc cgatgcctgg gtcagcagct cgttcagggc
     1381 gacggtatat ccggtgttcg gacgactggt caggcgaatg cgatggtccc gtgtggcgta
     1441 gcgctccagg atggcgcggg agcgatcggt cgatccgtcg tcgatggtca gaagctccca
     1501 atcgccccag ctttgaccga ggatgctgtc gaccgccttg gccacataac ggtcagcgtt
     1561 gtgcacgggc atcaggaccg agacggtcgg catccgcggt agtgctcctc tcgtgaaatc
     1621 gtccaatttg ccgaatatag gggtggctct tcggttgtgc ttcccggacg gccttatcct
     1681 tgcagcgggg tccgcggatc attttcgtat tcacgccgga aagcggagcc gatgactgac
     1741 cgtgctaatg gcggccagac cgtgagctgc gacctctcga tcctggtttc ccgcatcgac
     1801 accagcttca tgctcaagac catcccgcat ctcgtacggg cctgccgttt cccgttcgcg
     1861 cgccgggtgc tcgtaacgga cacggcgccg ctcggacggc ggtacgccag gcgtccgggg
     1921 atcggaacca tggaggatct ccgcgcctgt tgtgcgcaac tggttcaagc gggaatcatc
     1981 gacgtcatcg caccgatcga ctactcaccg ggataccacc ggcggatgta tcggaagcat
     2041 ttttccggct tgatgcggca gacccacagc aacggcggct atcctatttt gggcagcatc
     2101 ttcgctgtcg aggaaagtac cgcggactat ctggttcatt tcgacagcga catgctcctg
     2161 taccaagcgc ctgggttcaa ttggatcgag gagggcatcc ggctgctgca gcggcatgaa
     2221 gatctcatcg cggtgcttcc ccgctcgggg ccgccggctg cggatggccg gctcaagcag
     2281 caggaagaga ccggcgagcg gttcgagcag gattcccggg ggctgttctg cttccacacc
     2341 tttaccagcc gggtgttcct gatcgaccgc aagcgattcg agcgagtgct ccctttgcgt
     2401 ccgaggatgc catttccgga gctgttgcga aactatttca ccaagcgcaa taccatgcct
     2461 gaatgggaag tcatggtcgg gtaccggttg ccggaaacgg ggttggttcg ggcggacctg
     2521 agcacacccg acgcgtggac cattcacccg aacgatcgcg gcgagcagtt tgacgccgtg
     2581 cttcccaacc tgatcgcccg ggtcgaggcg ggggatttcc cgcctgatca aggcgggtac
     2641 tatgatctgc ggctcgatcg atgggccgca gcgtcttgac cccggatcag gtcatgactg
     2701 atacgcctct ggtttcagta gtcatgccct gcctcaatga ggaggcggcg atcggggcct
     2761 gcatcgagaa gatccaacgc accttcgcgg cggccgggat ctcgggcgag atcgtcgtgt
     2821 gcgacaacgg ctcgaccgat gcctccgtgg cgatcgctga gcggatggga gccagagtgg
     2881 tccatcaacc ccggcgcggc tacgggaacg catatctcaa gggattcgct gaagcgcggg
     2941 gtgagtatct gatcatgggg gatgccgacg acacctacga cttctcgctc atcccccagt
     3001 ttctcgacgg gctgaggaac caggggttcg acttcgtcac gggtagccgc tatctggccg
     3061 gtggtgataa gcacgtgacg ggcctgcatc ggttcgtcgg caatccggcg ctcaccgcga
     3121 ttctcaattt cctgttcagc acccgctaca ccgacgtgta ttgcgggttc cgtgggttca
     3181 gccgagccgc atacgaccgg attcgcccag tgagtcccgg catggagttc aacctcgagc
     3241 tggcgattaa cgccaggctt gccggcctca agatctcgga gatcccgatc gagctcgggc
     3301 ccaggaaggg tgagtccaag ctgcggacgt tccgggacgg gtggcgcagc ctgcgcatga
     3361 tgctgctgta ttcgccgaac cagctcttct tcgctccggg tggaatcctg ctcggctgcg
     3421 gcctggtgat ccacgtcctc gtcctgcttg gaatagtcaa attcggtggg cggccagcgg
     3481 cgggggttac cggggtcttc gcgaccattc tcagcgtggt cgggtttcag attttgagcc
     3541 tgggactcca cgccaagacc tattcgtgga gccgacggtt cgatctgaac aatcgggcgc
     3601 tggccgcgtt ctacgaccgc ttcaagctcg aggaaggcct tcttctgggt gccgggttgg
     3661 tgctcctagg ggctggcatt ctgacggccc tcgtgatcga atggctcagg ggcgacctct
     3721 tgccactgcc acggcctgag tgggcctcgt ttggagccac attggtcatc atcgggttta
     3781 gcaccatctt cacgtcgctc ttcatatcgg ccatgtccat caacaagcca gcggcctagg
     3841 gtgtcggatg gctcaagctc tcattagggt cggacgggcg gtgtatcacg cggtctcgcg
     3901 gaagcgcgag atcgtctacg tgccctggtt ctggcgaccc atcatgctcg ccgtgaggct
     3961 cgtgcctgag cgcgtcttca agcggctgca tttttagagt ggcgtccaac catcacccgc
     4021 tgggcgagga cgaccggtga gaaaaggcat tatcctggcc ggtggttccg gcacccgcct
     4081 ctggccctcc acgcgggtcg tcagcaagca gctgctcccg atttacgaca agccgatgat
     4141 ctattacccg ctcagcacgc tattgctggc gggcctccgg gacattctgg tgatcagcac
     4201 cccggacgat ctaccccgct tttcggagct gcttggggat ggggagcgct tgggcgtctc
     4261 gctcagctac gcggctcagc ctcggccgga ggggctggcg caggcgtttc tcatcggcgc
     4321 caattttctg aatggcgccg ccacaaccct gattctgggc gacaatctgt tcttcgggca
     4381 tggcatgccg gagcagctcc agcgcgccgg atcggcgagc acgggcgcta cgatctttgc
     4441 gtaccacgtg cgcgaccccg agcgatatgg ggtcgtggag tttgacgacg cggggaaggc
     4501 cttgagcatc gacgaaaagc cggcccgtcc gcggtcccag tacgcggtcg tgggcctcta
     4561 tttctatgac gcggaagtgg tctcattggc ccggcggctc aagccgtctg cccggggcga
     4621 gctggagatc acggacctca accgtgcgta cctcgaggcc ggccggttga cggtcgagtt
     4681 gctcggccgc ggcgttgcct ggctggatac cggtacgcac gatgcattgc tacaagcctc
     4741 ccacttcgtc agtgtcgtcg aggagcgtca gggcctcaag a
//
LOCUS       DASRJQ010000558         2903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2905073, whole genome shotgun sequence.
ACCESSION   DASRJQ010000558 DASRJQ010000000
VERSION     DASRJQ010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2905073"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VFB89_12745"
     CDS             <1..906
                     /locus_tag="VFB89_12745"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HZM28209.1"
                     /translation="VVHGGIHHWEPTPITPELLTELESLVPVDPEHMPQAVALMRAMT
                     RKNPKLPQIACFDTGFHHGMPLVARTYSLPRRLTAEGFIRYGFHGLSYEYVIQRLREI
                     DASAASGRVIVAHLGNGASLAALYQGRSIDTTMGFSPLGGLVMGTRCGDLDPGLVLYL
                     LRHEKLSPDQLNELLSHESGLLGVSELSSDMRDLLEHEVVDPRAAEAINLFCYQAAKY
                     IAAYVAALGGLDLLVFTGGIGERAAPVRRRICKRLEFLGLELDPDRNQANDRVISAAA
                     SKIVVRTLKTNEELMIARHACRFAM"
     gene            920..>2903
                     /locus_tag="VFB89_12750"
     CDS             920..>2903
                     /locus_tag="VFB89_12750"
                     /inference="COORDINATES: protein motif:HMM:NF003619.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HZM28210.1"
                     /translation="MTTLVFDPKVSSSVPQEAASVHTVPPPEGPLSAEELDRIHRYWS
                     ATNYLAVGQIYLMDNPLLHAPLAPEHIKPRLLGHWGTTPGLNFIYVHLNRLIRERDAD
                     VLFIAGPGHGGPALNANAYLEGSYSEVHPRITRDVAGMRLFFREFSTPGGVPSHCGPH
                     VPNSIHEGGELGYSLLHAYGAVFDNPNLLAVCVIGDGEAETGPLEGSWKSNKFLNPVR
                     DGAVLPILHLNGYKIAGPTVDGRTGDADLMWRFRGHGYEPLFVEGDDPATIHQLFAGA
                     LEACYHEIGVIQQEARRNGFHTRPTWPMIVLRTPKGWTGPKEVDGLPIEGTFRAHQVP
                     IEHVRSNPDHLRLLEAWLKSYRPDELFDADGRLIAGLSELIPEGDRRMGATPHANGGR
                     VLRPLELPDFRDYGLEVKAPGGFEAESPRKLGEFLRDVIRRNQANFRFFCPDETNSNR
                     LNAIFEVTDRASVAPTVPIDDHVGPDGRVMEVLSEHCCQGWLEGYLLTGRHGAWSSYE
                     AFAQIVDSMLTQHAKWLEQCIELPWRAPLASLNVFLTSHAWRNDHNGFSHQAPGFVDN
                     ALGRRSQVIRIYFPPDANCLLSVFDHCLRSRNYVNVVTCGKQPQLQWLSMDRAVEHCT
                     RGASRWEFASNDGDGQPDVVLGCAGDVPTLETCAASW"
BASE COUNT          530 a          903 c          907 g          563 t
ORIGIN      
        1 gtagtgcacg gtgggatcca ccactgggag cccaccccga tcacgccgga acttctgact
       61 gagctcgagt cgctcgttcc ggtcgatccg gagcacatgc cccaggcagt ggcgttgatg
      121 cgcgcgatga cgcggaaaaa cccgaagctg ccacagatcg cctgcttcga cacgggattt
      181 caccacggaa tgccgctggt cgcccggacc tacagcctgc cccggcggct gaccgcggaa
      241 ggattcattc gctacggctt ccacggtctc tcgtacgaat acgtgattca gcgattgcgt
      301 gagatcgatg cctcggccgc gtccggccgg gttatcgtgg cgcacttggg aaatggcgcg
      361 agcctggcgg ccctctatca gggtcgcagc atcgatacga ccatgggttt ttccccgctc
      421 gggggactcg tgatggggac ccggtgcgga gatctggacc ctgggctcgt gctgtacttg
      481 ctccggcatg aaaaattgag ccccgaccag ctgaacgagc tgctcagcca tgaatcaggc
      541 ctgctcggtg tgtccgaact cagctccgac atgcgtgacc tcctggagca tgaagttgtc
      601 gacccgcgag cagcggaagc catcaatctt ttctgctatc aggcggcgaa atacatcgcc
      661 gcgtacgttg cggctttggg tggcctcgac ctgctcgttt ttactggggg cattggggag
      721 agggccgcac cggtccggcg gcgtatctgc aaacgcctgg aattccttgg gctcgagctt
      781 gatccggacc gcaatcaggc caacgaccgg gtcatttccg ccgctgcaag caagatcgtt
      841 gtccgaacgc tcaagaccaa tgaagaactt atgattgcga ggcacgcgtg tcgcttcgcg
      901 atgtaggagc ggtcttccca tgactactct ggttttcgac ccgaaggtgt ccagctccgt
      961 gccccaggag gcagcgagcg ttcacacagt gccaccgcct gagggaccac tgtcggccga
     1021 ggagctcgac cgaattcacc gctactggtc ggctacgaac tacctcgccg tcggacagat
     1081 ctacctgatg gacaacccgc tgcttcatgc gccgctcgcc cccgagcata tcaagccgcg
     1141 gcttctgggc cactggggaa ccacgcccgg gctcaacttc atctacgtcc atctgaaccg
     1201 tctgattcgc gaacgtgacg ccgacgtgtt gttcatcgct gggcctggcc acggcggacc
     1261 ggcgctcaac gccaacgcgt atctggaagg cagctacagc gaggttcatc cccgaattac
     1321 gcgggatgtg gccggtatgc gcctcttctt ccgggagttc tcgacgccgg gcggcgtgcc
     1381 gagccactgc gggcctcatg ttcccaactc gatccatgaa ggcggagagc tggggtactc
     1441 attgctgcac gcgtacgggg ctgtcttcga caaccccaac ctgctcgcgg tgtgcgtcat
     1501 cggagacggc gaagccgaaa ccggtccgct ggaaggaagc tggaagagca acaagttcct
     1561 caacccggtc cgcgatggcg ccgtgcttcc cattctgcat ctcaacggct acaagatcgc
     1621 cgggcctacc gtggacggcc gcaccggcga cgctgacctc atgtggcggt ttcgcggcca
     1681 tgggtatgag ccgctgttcg tcgagggaga cgatcccgcc accatacacc agctgtttgc
     1741 cggtgccctc gaggcgtgtt atcacgagat cggcgtgatc cagcaggaag cccggcgcaa
     1801 cggattccat actcggccca cctggccgat gatcgtgctg cgaacgccta aaggttggac
     1861 cggtcccaag gaggtggacg ggttaccgat tgaaggcacg ttccgggccc atcaggtccc
     1921 gatcgagcac gtccgcagca acccagatca cttgcgattg ctggaggcct ggctcaagag
     1981 ctatcggccg gatgaattgt ttgatgcaga cggacgattg attgcagggc tgagcgagct
     2041 gattcccgag ggggatcggc gaatgggtgc gaccccgcat gccaacggag gtcgtgttct
     2101 acggccgctc gagcttccgg atttccgcga ttatgggctc gaggtcaagg cacccggcgg
     2161 cttcgaggcg gagtcacccc gcaagctggg cgagtttctc cgcgacgtga tccggcgcaa
     2221 tcaggccaac ttccgattct tttgtccgga cgagaccaat tcgaaccggc tcaacgcgat
     2281 tttcgaggtg accgatcgcg ccagcgtggc gccaaccgtt cccatcgacg atcacgtcgg
     2341 accggatggt cgagttatgg aggtgctgag cgagcactgc tgccagggat ggctcgaggg
     2401 ctacctgctc accggacggc atggcgcctg gtcgtcgtac gaggcgtttg cccagatcgt
     2461 ggactcgatg ctgacccagc atgccaaatg gctggagcag tgcatcgagc ttccttggcg
     2521 agcgccgctg gcgtcgctca acgtctttct cacgagtcat gcctggcgca acgaccacaa
     2581 tgggttcagc catcaggctc ccgggtttgt cgataacgca ctcggacggc gcagccaggt
     2641 gatccggatc tacttcccgc cggacgccaa ctgcctgctc tcggtattcg atcactgtct
     2701 taggagccgg aactacgtga acgtagtcac gtgcgggaaa cagccccagc tgcagtggct
     2761 ttcgatggat cgtgccgtcg agcattgcac ccgaggcgct tcgcggtggg agttcgccag
     2821 caacgatggc gacggacagc cggacgtcgt tctgggttgt gcgggcgatg ttcccaccct
     2881 cgaaacctgt gcggcctcgt ggc
//
LOCUS       DASRJQ010000559         4360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_366210, whole genome shotgun sequence.
ACCESSION   DASRJQ010000559 DASRJQ010000000
VERSION     DASRJQ010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366210"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1773
                     /locus_tag="VFB89_12755"
     CDS             <1..1773
                     /locus_tag="VFB89_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28211.1"
                     /translation="PELPKELIEAVDRAIAKKPNDRFQTVKEFAGALDVVAANAVATS
                     PTPPDAYARAAASGVRRAIEERNESERRGSKSGALLLAAAALAALALAATTLFPWGKN
                     AKPPRPEPTAAPVGRDPPVSAGPAYRSTIAVMPLVAYSHDSQSTFLSEGLTEEIITQL
                     AQIKNLKVISRTSVVALASKGLTVPQIAETLGVRHVLEGSVQRAGDRIRVSLQLIDAT
                     NDAHLWAQSYDEKLKDAISLRADVAGKVASALAMTVPAIEPAPADSHAAPENSAAYDA
                     YLRGSYWVARVNLASTERAIKAFEDAIRADSGFAPAYAGLSKAQQQMVAIARSGDLYA
                     TLSRALANVERALQLDSNLAAGYLARATIRLNAWAPDSLVEADIGRAMASMPNSANTR
                     IVHSLALARQGRYAEAQAEADAAVQLDPLSPAMHTAVALVALAARRYDLAVNESRRTL
                     SLDSQFVGGYAIEAVALLEQRQPDRCLALDLRAFPEIRAMCLQAAGRRAEAAGLADSV
                     STAYAAGNYLSVFQLGLLGAYYGQQGDTRRAVQWLERGYAVSPNPVEFRVLDSALFDR
                     AREDPEYRAALARLHRQVYLRVFR"
     gene            1770..2894
                     /locus_tag="VFB89_12760"
     CDS             1770..2894
                     /locus_tag="VFB89_12760"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZM28212.1"
                     /translation="MRFGIVALGLVAACAGAGPSSVPRPAPNAVELTMFLIGDAGAPA
                     PGGEPVLKALSAELAKAPGQRVVVFLGDNVYPRGLPDSAAPDRTEMERRLDTQVDVVK
                     AAGARGIFVPGNHDWAKHGPDGWNAMRRQGRQIARRGAPEVELLPVDGCPGPAVVDFG
                     ERVRLIALDTQWWLHGGPRPVGPDSKCRAANERAVADSLKADLASAGNRKVVVAAHHP
                     LATGGEHGGYFGVQSYLFPLRVVKPWLWIPLPGIGSTYPIARQSGVSNQDMAGMLNQR
                     MRDMLQRAMRDHPPLVWADGHDHNLQVLKGLSARWLLVSGAGIFGHEGPVNRLPETRF
                     ASQDAGYMRVEFLTDGRERLGVLAVDREGRSLERFSAWLE"
     gene            2912..>4360
                     /locus_tag="VFB89_12765"
     CDS             2912..>4360
                     /locus_tag="VFB89_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28213.1"
                     /translation="MRIRPITLVLGSALAAAPLVAQSPRDSLTPGSTVRVAVDPKYEK
                     GGLHRFFFGNHYRDLWATPIEIPVLDLKTYAGGLKPVRRGGGKQTKSLHLESGDGRKW
                     AFRSVDKDPSPLLPPELRNTLADRMFHDQISAGHPAGPLVIGPLLEAVGVKVASVQLF
                     VMPHDPALGEFSDFGGVVGTLEERPTTGDDDSPGFEGAVKVADTEELFERLGKDPREH
                     IDTVAFLKARLMDILLGDWDRHEKQWRWIKLEEGKDAPWLPVPYDRDQAFVRYDGLLL
                     SVARAGGFPQLVNFGKDYPSSVGITWNPRFLDRRLLVGLPQSVWESSAREIQGRLTDS
                     VIDAAVHRLPPEYYKLDGERMATTLKQRRDGLPNEADRLYRFYARDVDVRTTKEDEVA
                     VARRNGDGTMDLSIRERDSEKYDSAPFFHRRFKSGETAEVRLYLSKGRDSVHVEGDGG
                     GPKLRVIGTDSAGTGDEPNSVVDDASGGSTKVY"
BASE COUNT          743 a         1408 c         1459 g          750 t
ORIGIN      
        1 ccggagctgc ccaaggagtt gatcgaggct gtggaccggg ccatcgccaa gaagccgaac
       61 gaccggttcc agacggtgaa ggaatttgcc ggcgcgctcg atgtggtggc agccaatgcg
      121 gttgccacca gccccacacc tccggacgca tacgcccgcg ccgccgccag cggagtccgg
      181 cgggcgatcg aggagcgaaa tgagtccgag cgccggggca gcaagtcggg tgcgctgctg
      241 ctggcggccg ccgcgctggc tgctctggcg cttgcagcca ccacgctgtt tccctggggc
      301 aagaatgcta agcccccgcg gccggagcca accgcagcgc ccgttgggag ggatcccccg
      361 gtttcagccg gaccggccta tcgctcgacc attgcggtga tgccgctcgt ggcctacagc
      421 cacgacagtc aatcgacctt tttgagtgag gggctcaccg aagagatcat tacccagctg
      481 gctcagatca agaacctcaa ggtcatttcg cgaacctcgg tcgtcgcgct tgcctccaag
      541 ggcctgaccg ttccccagat cgcagaaacg ttgggcgtgc gccacgtact cgagggctcg
      601 gttcagcggg cgggggatcg aatccgggtc tcgctgcagc tcatcgacgc aaccaatgat
      661 gcgcatctct gggctcagtc ctacgacgaa aaattgaagg acgcaatctc gctccgcgct
      721 gacgtcgccg gaaaggtggc gtcggctctc gccatgaccg tgcccgccat cgaaccggca
      781 cccgcggact cccacgccgc tccggaaaac agcgccgcgt acgacgccta cctccggggc
      841 agctattggg tggccagagt gaacctggcg agcaccgagc gggccatcaa agcgttcgag
      901 gacgccatcc gggccgattc agggttcgcg cccgcgtacg ccggcctctc gaaggcgcag
      961 cagcagatgg tcgccatcgc acggagcggg gacttgtacg ccactctgtc gcgggcgctg
     1021 gcaaatgtgg aacgtgcgct tcagctggat tccaatcttg ccgccggcta tctcgcgcgc
     1081 gcgaccatcc ggctcaacgc ctgggcaccc gattctctgg tggaggccga tatcggtcgc
     1141 gccatggcga gcatgcccaa ttcagccaac acccgcatcg tgcactccct cgcgctcgcg
     1201 cggcagggac gctacgccga ggcccaggcc gaagcggacg ccgccgtcca gttggatcca
     1261 ctgtcgcctg ccatgcacac ggctgttgcg ctcgttgcgc tcgcagcgcg gcgttacgat
     1321 ctggcggtca acgaaagccg ccggaccctg agcctggatt cccagttcgt cgggggctac
     1381 gccatcgagg cagtcgcctt gctagagcag agacagccgg accgctgcct ggctctcgac
     1441 ctgcgcgcat tccccgagat tcgggcgatg tgcctccagg cggcaggacg gcgggctgag
     1501 gcggccgggc ttgcggactc cgtcagcact gcgtacgccg ccggcaacta cctgagcgtg
     1561 ttccaactcg gcttgctcgg ggcctactac ggccagcagg gcgatacgcg ccgcgcggta
     1621 cagtggctgg agcgaggcta cgccgtctct cccaacccgg tcgagttccg tgtgctcgat
     1681 tcggcgttgt tcgatcgggc tcgcgaagac cccgagtatc gggcagcact ggcccgtctg
     1741 caccgccagg tctacctccg cgtctttcga tgagattcgg tatcgtcgcg ctcggcctcg
     1801 tcgccgcatg cgccggggcg ggcccgtcat cggtgccgcg accggcgccc aacgcggtcg
     1861 agctcaccat gtttctcatc ggcgacgccg gagcacccgc gcccggcggg gagccggttc
     1921 tcaaagcact gagcgctgag ctggcgaagg ctcccggtca gcgcgtcgtc gtgtttctgg
     1981 gtgacaacgt ctatccccgc ggacttcccg attcggctgc gcctgaccga accgagatgg
     2041 agcggcggct cgacacgcag gtggacgtcg tgaaggccgc gggagcgcgg gggattttcg
     2101 ttcccggcaa ccacgattgg gcgaagcatg ggccggatgg ctggaatgca atgcggcggc
     2161 aggggcggca gatcgcgcgc cgcggtgcac cggaggtcga gcttcttccg gtggacgggt
     2221 gccccggtcc ggccgtggta gactttggcg agcgggttcg cctgatcgcg ctcgatacac
     2281 agtggtggct ccacggaggc cctcggccgg tcggacctga ctccaagtgc cgcgctgcca
     2341 atgagcgtgc tgtcgccgac tcactcaagg ctgatctcgc atcggccggc aaccgcaagg
     2401 tcgtggtcgc tgcgcatcac ccgctggcga ctggaggcga gcacggaggg tacttcgggg
     2461 tccagtcgta cctctttccc ctccgggtgg tgaagccctg gctctggatc ccgcttccgg
     2521 gtatcggatc gacctacccg atcgcgcggc agagcggagt atcgaatcag gatatggccg
     2581 gaatgctgaa tcagcgtatg cgggacatgc ttcaacgagc catgcgggac catccgccgc
     2641 tggtctgggc cgacgggcac gaccacaatc tccaggtcct caaaggcctg agcgcccgct
     2701 ggctgcttgt cagtggagca ggcatctttg ggcatgaggg tccggtgaat cgcttaccgg
     2761 agacccggtt tgcttcccag gacgccggat acatgcgggt agagtttctt accgacggtc
     2821 gagaacgcct gggcgtgctc gccgtggatc gggagggtcg ctcgctggag cgattctccg
     2881 cctggctcga atgaacaggc tggaggacat gatgcgcata cgccccatca cgctggtcct
     2941 cggcagtgcg ttagcagcag ccccgctggt ggcgcagtcg ccgcgcgact cgctgacacc
     3001 cggctcgaca gtcagggtcg ccgtggaccc caagtacgag aagggtgggc tccaccgctt
     3061 cttcttcggc aatcactacc gcgacctctg ggccacgccc atcgagatcc cggtgctgga
     3121 tctcaagacc tacgccgggg gcctgaagcc ggtccggcgg ggcggcggca agcaaaccaa
     3181 atcgctgcat ctcgaaagcg gggacggccg caaatgggca ttccggtcgg tcgacaagga
     3241 tccatcgccc ctgctacctc cggaacttcg caacaccctc gccgaccgaa tgttccacga
     3301 ccagatcagt gctgggcatc cggccggccc cctcgtcatc gggccgttgc tcgaagcggt
     3361 cggcgtcaag gtcgcgtcgg tacagctctt cgtcatgccg cacgatccgg cactaggtga
     3421 gttcagcgat ttcggtggag tggtcggcac tctcgaggaa cggccgacga ccggtgatga
     3481 tgattcaccg ggtttcgagg gcgccgtaaa ggtcgctgat accgaggagc tgttcgagag
     3541 gctcggcaag gatccccggg aacacatcga tacggtcgca tttctcaaag cccgtctcat
     3601 ggacatcctg ctgggcgatt gggatcgcca tgagaagcag tggcgctgga tcaagctgga
     3661 ggaggggaag gatgcgccct ggctgccggt tccgtacgac cgcgatcagg cattcgtccg
     3721 ctacgacggt ctactactct ctgtggcgcg ggccggtggc tttccccaac tggtcaattt
     3781 tggcaaggac tatccgtcgt ccgtcggcat cacctggaat ccgcgatttc tcgatcgccg
     3841 gctactggtc ggcttgccgc agtccgtctg ggaatcgtcc gctcgtgaga tccagggtcg
     3901 gttgacggac tcggtgatcg atgcggcggt ccatcggctg ccgccggagt actacaagct
     3961 ggacggcgag cgtatggcga cgacgctcaa gcagcggcgg gacgggctgc cgaacgaggc
     4021 cgaccggcta taccgcttct acgcgcgcga cgtggacgtc cgcacgacca aggaggacga
     4081 agtcgccgtg gcgcgccgga acggcgatgg caccatggac ctgagcattc gtgagcggga
     4141 ttcggaaaag tacgacagcg ccccattctt ccatcgccgg ttcaagagcg gcgagaccgc
     4201 cgaggtccgt ctctatctca gcaagggaag ggatagtgtg catgtggaag gtgacggagg
     4261 cggtcccaag ttgcgggtca tcggaacgga cagtgccggg actggtgacg agcccaactc
     4321 cgtggtggat gatgcaagcg gcggctcgac caaggtgtac
//
LOCUS       DASRJQ010000560         2002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1515087, whole genome shotgun sequence.
ACCESSION   DASRJQ010000560 DASRJQ010000000
VERSION     DASRJQ010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1515087"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VFB89_12770"
     CDS             <1..221
                     /locus_tag="VFB89_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28214.1"
                     /translation="SGARSSVGIKQFEAGGGGKSHHTMKPFNDKIDGTKRFNDVFAVL
                     RLDLHPGSYSYRFVTPAGDRFTDSKPCV"
     gene            complement(556..1413)
                     /locus_tag="VFB89_12775"
     CDS             complement(556..1413)
                     /locus_tag="VFB89_12775"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HZM28215.1"
                     /translation="MTARTRDRGLASGRIAVMINDWIEDRFTLPKPDLPSLTGHAPEP
                     ATQELRARWGLGEKPIGNLIHLLEAHGVRIYSLTHENTDLDAFSLYWHGQPFMFLGTE
                     KSAERSRFDAAHELAHLVLHGEEREPYGAAAEREANQFAAAFLMPRSGVLAQRLRNAP
                     AERILVAKRRWNVAAMALAHRTNELGLMTEWCYRSVCINLSRMGYRRGEPAGMGARES
                     SQLLTKVLQQLRTAGSGVHEIAGDLGLQPSEVQAHMFGLTPIVLTGGDSSGGSTPSRP
                     GLHIVRGGT"
BASE COUNT          344 a          628 c          662 g          368 t
ORIGIN      
        1 cgtcgggcgc ccgctcctcg gtgggcatca agcagttcga ggcgggcggg ggcggcaaaa
       61 gtcaccacac gatgaagccg ttcaacgaca agatcgacgg caccaagcgg ttcaacgatg
      121 tgttcgctgt gctgcgcctg gacctgcatc cggggagtta cagctaccgg ttcgtgacgc
      181 ctgcgggtga ccggttcacc gatagcaagc cgtgcgtctg atgacgttcg cggcgtacgc
      241 ggcctggatc gcggcagccg cgcctaccgg ctagcaagat cactccgaag cgggagccct
      301 ggaccaatcg aggtccgggg ctctcagcat ttcccgcaga ttcgtaccgg cgtcagcgag
      361 ggctgacgca acgcgagcct gatggtgcgt tcgttctcca cggcggccag catcaattcc
      421 ctgaggtcac ctgtgaagtg gcggaaccgt gacgctatcc gacgcgcaca tcgtgggtcc
      481 cggagcggca atgcgctctt gaacaacgac gcgtatcccc tgcgacgcga tcgaacctac
      541 tgtgagcgct gcgagtcacg ttcccccgcg gactatatga agccctggcc ggctgggcgt
      601 actgccaccg ctagagtcgc caccggtcag gactatgggt gtcagaccga acatgtgagc
      661 ttgaacctcg ctcggctgaa gaccaaggtc gccggcaatc tcgtgaacgc cggaacctgc
      721 agtgcggagc tgctgaagga ccttagtcaa cagttgagag gactcgcggg cgcccatacc
      781 agctggctcg cctcgccgat agcccatccg cgacaggttg atacagacgc tgcggtagca
      841 ccactcggtc ataaggccaa gctcattggt gcggtgagcc agagccatgg cggcaacgtt
      901 ccagcgtcgc ttcgctacaa ggatccgctc tgccggagcg tttcggagcc tctgggctag
      961 cacccctgag cgcggcatga gaaacgccgc ggcaaactgg ttggcttcgc gctcggccgc
     1021 cgcgccgtaa ggttcgcgct cctccccgtg gagcacaagg tgcgctagct cgtgtgcggc
     1081 gtcgaaacgg ctgcgctctg cggacttctc ggtgccgagg aacatgaacg gctgcccatg
     1141 ccagtaaagg gagaacgcgt cgaggtcggt gttttcgtga gttagggagt agatccgtac
     1201 gccgtgcgcc tcgagaaggt ggatgagatt tccgatcggc ttctctccga gcccccatcg
     1261 ggcgcgcagc tcttgggtgg cgggctcagg tgcgtgtccg gtgaggcttg gcaggtccgg
     1321 ctttggaagg gtgaagcggt cctcgatcca gtcattgatc atgacggcga tgcggccaga
     1381 ggcgagccct cggtcgcgtg tgcgggcggt catccacgtc atacagcacg gccggcaccg
     1441 ccggcctgct ttcagcgaca gccatgattg ctctcccttc ctcggtgcca ccaccggctt
     1501 gcctccggtc cctgcgcggt ctgcgttgcc cacctgatcg gcgaacatct cgggccgggt
     1561 cgggccagtt accggtgcgc cggatcgggg accgctcctc ctccgaatgc ggatgtcctc
     1621 atcaagccaa acggagcacc gatcgggcgc catgcgcgac gacctgtgga taggaccgcc
     1681 ggccggacgg ctcattcccg tcacatgtcc ctaaggacgc gggtgctgcc tggcggtcag
     1741 ctgactcacc atcgattggg taactgggtg attccgacgg caaatgtctg acgccatttg
     1801 ggtctgtcca ccgatgctta tcgcactgtc gagttatgcg aagttcgcgg tctgcggcca
     1861 gagtaccgac cagggctgtc gcaggccggt gcaccggtgg atcggggtgc cggtcaggtc
     1921 attgtccacg ccgggcggtg tctggaccgt gcccgccaac tcgcagtgtg tgtacccggt
     1981 cgcgagatcg gccggggaga aa
//
LOCUS       DASRJQ010000561         2246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1210983, whole genome shotgun sequence.
ACCESSION   DASRJQ010000561 DASRJQ010000000
VERSION     DASRJQ010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210983"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VFB89_12780"
     CDS             complement(<1..507)
                     /locus_tag="VFB89_12780"
                     /inference="COORDINATES: protein motif:HMM:NF021128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HZM28216.1"
                     /translation="MSVSQGYKKCAAVDRKQFIGGSDVRIIMGDDEAALLRLWREKRG
                     ETEPEDLSGNLLVQLGLATEHLNRQWYERTTGQVVKDVQSWVRHQVIRWMAATLDGIV
                     EGTGEVFEAKFMLPWSFSEEAAAEKHMAQLQHNMWVSNATAAVLSIITGGGKWVEITI
                     PMDPLYVTV"
     gene            complement(979..>2246)
                     /locus_tag="VFB89_12785"
     CDS             complement(979..>2246)
                     /locus_tag="VFB89_12785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HZM28217.1"
                     /translation="NILAGLIVVIILAGGVGGWAATAQISGALIAPGSLVVDSNVKKV
                     QHPTGGVVGEVRVRDGDRVKAGDVVVRLDETITRANLAIVTKGLTELYARKARLAAER
                     DGAGSIPMPKELANRANDPDVAEALSSERKLFDLRRTSRSGQKAQLAQRVAQLQEEIT
                     GLTTQQKAKAQEIVLINRELVGVRELWEKKLIPLSRLTQLEREATRLDGERGQLVAAA
                     AQARGKVTETELQIIQIDQDLSSEVAKEMREIDGKIGEFIERKVAAEDQLKRIDIRAP
                     QDGYVFQSTVHTVGGVITPGEAIMMIVPEADNLKVEAKVDPKDIDRLQIGQPVVLRFV
                     TFNQRTTPELNGTVTLIAADTSNDQRTGVSYYTIRIGMTVQEIARLGDVKLAPGMPVE
                     AFVQTGERTMFSYLMKPLHDQLMRAFRER"
BASE COUNT          378 a          722 c          695 g          451 t
ORIGIN      
        1 gaccgtaacg tagagcggat ccatgggaat tgtaatttcc acccactttc caccgcccgt
       61 gatgatcgac agcaccgcgg cggtggcatt gctgacccac atgttgtgct ggagttgggc
      121 catgtgcttc tccgccgccg cctcctccga gaacgaccac ggcagcatga acttggcttc
      181 gaacacctcg ccggtgcctt cgacgatccc gtccagcgtc gccgccatcc agcggattac
      241 ctggtggcgg acccagcttt ggacgtcctt gaccacctgc ccggttgtgc gctcgtacca
      301 ctgccggttc aggtgctcgg tcgcgagccc cagttggaca agcagattgc ccgacaggtc
      361 ttccggctcc gtctcgcctc gcttctcccg ccagaggcgg agcagagcag cctcatcgtc
      421 gcccatgatg atccggacgt cggagccgcc gatgaattgt ttacgatcca ccgcagcaca
      481 cttcttgtag ccctgtgaaa cagacacgac tgccttcctc ccttatgtgc tatcatcttg
      541 tgcgatttgg acatgtgaca atagcacaag ccagtgcctg tgacaagtgc acaattgaga
      601 atggctcgag cagccctgaa ttggacggtt cgggatcttg ccgaagcgac cggccttcac
      661 cgcaatacga tcaccaacat cgaggttggg cgctatgcag gggacgagaa gtcactggaa
      721 atgatagagg ctgtcctaaa gaaggccggc gtggtgttca ttgatgagaa cggcggcggg
      781 cccggggtgc gcctgcggag gtcacctcaa cacgggaaac gcaaataagg cctgggttta
      841 ccaaggccca atcttggttc cagccccgca atacggacgg ccgatgacat gcaattggct
      901 caatatttgc gtgacagccc cggcggctca cgttgcacct cgccggctcg ccggaaaaaa
      961 atgttatggt ggtgccgttc acctctcgcg gaacgcccgc atcagctggt cgtgcagggg
     1021 cttcatgaga taggagaaca tcgtccgctc accggtctgc acgaacgcct cgaccggcat
     1081 gccgggcgcc agtttgacat cgcccagccg ggcaatctcc tgcacggtca tcccgatgcg
     1141 gatagtgtag tagctcactc cggtgcgctg gtcgttcgac gtgtcggccg cgatcagggt
     1201 gaccgtgccg ttgagctcgg gcgtggtccg ctggttgaag gttacgaagc gcagcaccac
     1261 cggctggccg atctgcaggc ggtcgatgtc cttggggtcg accttcgctt cgaccttcaa
     1321 gttgtccgcc tcgggcacga tcatcatgat cgcctcgccg ggcgtgataa cgccgccgac
     1381 tgtgtggacc gtcgactgga acacgtaccc gtcctgcggc gcgcggatgt cgatgcgctt
     1441 gagttgatcc tccgccgcaa ccttgcgctc gatgaactcg ccgatcttgc cgtcgatctc
     1501 gcgcatctcc ttggccacct cgctgctgag gtcctggtcg atctggatga tctgcagctc
     1561 ggtctcggtg accttacccc tggcctgggc cgcggccgcg actaactggc cgcgttcgcc
     1621 atcgagccgg gtcgcctcgc gctcgagttg ggtgaggcgg ctgagtggga tgagcttctt
     1681 ctcccacagc tccctgacgc ccacgagctc gcgattgatc agcacgatct cctgcgcctt
     1741 ggctttctgc tgcgttgtga ggccggtgat ctcctcctgc agctgtgcga cgcgctgggc
     1801 gagctgggcc ttctggccgc tgcgcgacgt gcgccgcagg tcgaacagct tgcgctcgct
     1861 cgaaagcgcc tcggcgacat ccggatcgtt ggcgcggttg gcgagctcct tgggcatcgg
     1921 aatggatccc gccccgtcgc gctcggcggc gagccgggcc ttgcgggcgt agagctcagt
     1981 caggcctttt gtgacgatgg cgagattggc tcgcgtgatc gtctcgtcga ggcgcacgac
     2041 gacatcgcct gccttgacgc ggtcgccgtc gcgcacgcgc acctcgccga cgacgccgcc
     2101 ggtcggatgc tgcaccttct tcacgttcga gtcgacgacg agggagccgg gcgcgatgag
     2161 cgcgccggaa atctgcgcgg tggcggccca cccaccgacg ccgccggcga gaataatcac
     2221 aactattaga ccggcgagaa tgttgc
//
LOCUS       DASRJQ010000562         9228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_646350, whole genome shotgun sequence.
ACCESSION   DASRJQ010000562 DASRJQ010000000
VERSION     DASRJQ010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 9228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9228
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646350"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1293)
                     /locus_tag="VFB89_12790"
     CDS             complement(<1..1293)
                     /locus_tag="VFB89_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28218.1"
                     /translation="MSDYRLARPRSSHLCRAVAVVAWLSAAPALLAQATPSRFDLLIR
                     NGRIVDGTGSPWYSGDVAITAGRIAAIGRLGTVKSGKVIDAHGLIVAPGFIDMLGQSE
                     LTILVNPHVPSKIFQGITTEVTGEGTSVAPLNDRIIQADRVSYAHFQITPEWRTLSQY
                     FARLERQGIGINFATFVGATQVRRMVLGDENRTPNGAQLDSMKALVRQAMQQGAVGVS
                     TSLQYAPAPYATTDELIALAAEAGKFGGIYATHMRSEQAAVLTALDEAIRIGREARVP
                     VEIWHLKAAGKSNWGRMKEIVARIEEARRSGVDLTADTYAYPAWFNSMSAFVPPWAHD
                     GGDAKMIERLKDPAIRARIRKDMETPSGDWDNEWQEIPGPEAVLVGVVQNPKLLPVQG
                     KNLAEIAKQRGKDPIEVLFDILIEDEAYTYVAVFGMAEP"
     gene            complement(1286..1855)
                     /locus_tag="VFB89_12795"
     CDS             complement(1286..1855)
                     /locus_tag="VFB89_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28219.1"
                     /translation="MGPAQCVLPRIVYSGGMRPVARAGLVAGGYVAAFAIAWLVVRMY
                     IAATNVPDRQVYGAMYDFGDSLLFLGTLGLAAVPATGAALFFLRGRPAFWKILSITAL
                     AIACTSVAAFLIYLAPMVLSQRPMANSWSAFASLRILVAPLFALFFLLSWLFAPNRSA
                     RNALLTAGLIETAVFGYYLASTWFHSAHV"
     gene            complement(1916..2632)
                     /locus_tag="VFB89_12800"
     CDS             complement(1916..2632)
                     /locus_tag="VFB89_12800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HZM28220.1"
                     /translation="MTLEIGYRVVAFDVFGTVVDWRSGVIAAGERLAARHGLRVDWAA
                     FADAWRNAYSPGMDRVRRGELPWTKLDELHRRTLDALIPRFGLEAFPEADRDELNRAW
                     HALPPWPDAVEGLRRLRSRFTITTLSNGNVSLLTDMARNAGLPWDCILSAELVQHYKP
                     DPEAYLQVPRLLDVRPDQVLMVAAHANDLNAARQSGLRTAFIHRPLEWGAGKAPPQPA
                     KNQFDHEAANFLELAEQLGV"
     gene            complement(2708..2890)
                     /locus_tag="VFB89_12805"
     CDS             complement(2708..2890)
                     /locus_tag="VFB89_12805"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HZM28221.1"
                     /translation="MGKMVECAKVDPSSGCKHIIRGKTDDEVMKNAMEHAKQHGIREA
                     TPELMAKVKAAIRDEK"
     gene            complement(3003..3587)
                     /locus_tag="VFB89_12810"
     CDS             complement(3003..3587)
                     /locus_tag="VFB89_12810"
                     /inference="COORDINATES: protein motif:HMM:NF022216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HZM28222.1"
                     /translation="MSDRPWWAPAIQWGVWLVIMSLVMAWVARGRLPSTPTQGGQALV
                     HPRSTLIIGLVCAGLFLTIALLSALFPGKSGSPGITLFFVGFAALGLLMILEYRNGRH
                     TLTKDGLQYGRMIGQTGALRWNEVRRLAYSESAKWFRIELADGRVVRVSAMLVGLPSF
                     ARAALDHVPPTAIDPNTQSVLEATAAGNLPRIWG"
     gene            complement(3584..4432)
                     /locus_tag="VFB89_12815"
     CDS             complement(3584..4432)
                     /locus_tag="VFB89_12815"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZM28223.1"
                     /translation="MNQIPPSFDRSIAAYYDQAPEETRLEQLPFVLEEIRTRELIQRH
                     APAPPATVLDVGGGAGAYAMWLTEVGYAVHLLDASPRLVAEARRRAASLPRPLASCQV
                     GDARRLDFADSCADIVLLLGPLYHLTDAPDRALALGEAFRVLKPGGLLFAAVISRYAS
                     ALDGMSRDLFQDPRFRSIVEADLAEGQHRNPTERLDYFTTAYFHRPEDLRAEALGAGF
                     DVEGVYGLEGPGWLLPDLAARWADPRQRSDLLRLAGQLETESSILGISAHLLAVARRP
                     SRLQAA"
     gene            complement(4439..5080)
                     /locus_tag="VFB89_12820"
     CDS             complement(4439..5080)
                     /locus_tag="VFB89_12820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HZM28224.1"
                     /translation="MTLEPVHPEIRRYLDSLVPPRAPELAAMEAEAERAKFPIIGPAA
                     GHFCYAVSRLIGARSVFELGSGFGYSTAWFARAVRENGGGVVHHTVWDTELSKEARTR
                     LGTLGFADIVQYHVGEAVQTLREVRGSFDLVFLDIDKDGYPAALPVIRERLRPGGVLI
                     ADNLLWHGQVIDPKDHSEATAGVREFTRLVTTGQDWLASVVPIRDGLLLAYRR"
     gene            complement(5187..5594)
                     /locus_tag="VFB89_12825"
     CDS             complement(5187..5594)
                     /locus_tag="VFB89_12825"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZM28225.1"
                     /translation="MTGRDRPPGGDDQATAETLRATERERLRALISGDVTRASQFHTD
                     DFELINPLGGVLSKDQYLGSIGSGQLQYLVWEPDAIAVRVYGEVAVIRYQSQLEIVVQ
                     GRHIPRQRYWHTDVYERRGEQWQVVWSHATGIQ"
     gene            complement(5603..6403)
                     /locus_tag="VFB89_12830"
     CDS             complement(5603..6403)
                     /locus_tag="VFB89_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28226.1"
                     /translation="MSNQELEPKRLPVDERRVAVEERRVALEERKAASEDRFVRRNFA
                     TLATALISIATIFFSVVQWKVSSRKAELDAQQLAKRADREYDMETFKVVMASVSSKDS
                     AQQYAALSLVARMNDSTLRDALRDALQRGGTAPVRVAAAEKLAQEQQFKLDNERLRTP
                     GGTSKWRYDVFYCESSGPAAKPLADSIATRLRGEATVRTRALTSWINASPGYRVSGFQ
                     VRYEPSEEAQARPLLDRLNASSLMGQRQFVGQVVSNTTSRYLSIFVCP"
     gene            complement(6467..6856)
                     /locus_tag="VFB89_12835"
     CDS             complement(6467..6856)
                     /locus_tag="VFB89_12835"
                     /inference="COORDINATES: protein motif:HMM:NF025015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HZM28227.1"
                     /translation="MIVSRIAFPALGLATLIVGWPLAHRWVPPNRWYGLRVPATFADP
                     RIWYEANAVCGDDLVRLGAVLLVVALGLQFLRGLPELGYVTICLAVLVVGSLRATIRG
                     VKVARKLQRQQNPNGSRSTGSNGRASP"
     gene            complement(6887..8857)
                     /locus_tag="VFB89_12840"
     CDS             complement(6887..8857)
                     /locus_tag="VFB89_12840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HZM28228.1"
                     /translation="MRPRELTLRGLLLGALITTAFTAANIYLGLKVGLTVASSIPAAV
                     ISMAILSAFKDSSILENNIVQTVASAAGTLSAIIFVLPGLVIVGWWTGFPFWQSFLIC
                     VSGGVLGVLFTIPLRRALVTTSDLPYPEGVAAAEVLKVGSGTRGETKDETGEAREGLV
                     AVMLGSVASAGLAIITGTRIAAAEVVGFFRLGATASSGYNIAWSLALLGAGHLVGLSV
                     GMAMLTGLVISWAIAVPILTSMQPAAADTTLAAHTLTIWRTQVRFIGAGTIAVAAIYT
                     LATLAKPVVGGLVSTLAASRAKKVADDRDRDLSPSWIIGLTVACLVISAWLAFTFAKS
                     TVLASSALALTLVAVPFVLIVGFIIAGICGYMAGLIGASNSPISGVGILSIVVCASVL
                     ALTVSPTQDTRPALVAFALFVTAIVFACATISNDNLQDLKTGQLVGASPMRQQIALIV
                     GVGAGAAVIPFVLNLLAKAYGFAGAANVGVVAPNPLPAPQATLISALAQGVIGGKLEW
                     KMIGIGGLIGVGLILLDATLGAMKKLRIPPLAVGIGIYLPMSATFAVVVGAVLSHWYD
                     RRARQSPNPERADRLGTLVASGLIVGESLWGVINAGLIVGLSKEAPIAFVPETFGPAP
                     WLGLIGFVGVIVWLYGWMLRRSAAAQPASSAR"
BASE COUNT         1752 a         2898 c         3017 g         1561 t
ORIGIN      
        1 cggctccgcc atgccgaaca ctgcgacata ggtgtatgcc tcgtcttcga tcaggatgtc
       61 gaataggacc tcgatggggt ccttgcccct ttgcttggcg atctcggcca ggttctttcc
      121 ctgaacgggg agcagcttgg gattctggac gacacccacg agcacggctt cgggccccgg
      181 aatctcctgc cactcgttgt cccagtctcc gctcggcgtt tccatgtcct tcctgattcg
      241 ggcgcggatc gcgggatcct tcagccgctc aatcattttt gcgtcgccgc catcgtgagc
      301 ccacggcggc acgaaggcag acatcgagtt gaaccaggcc ggataggcgt aggtatcggc
      361 ggtgagatcc accccgctgc gccgagcctc ttcgatccgg gcgacgatct ctttcatgcg
      421 tccccaattc gacttgcccg ctgccttcag gtgccagatc tcgacgggaa cccgcgcttc
      481 ccgcccgatc cgaatggctt cgtcgagcgc ggtcaggacc gcggcctgct cggagcgcat
      541 gtgggtggca tagatcccac cgaacttccc ggcctccgca gcgagggcga tcaactcgtc
      601 ggtggtcgcg taaggggccg gtgcgtactg caatgaggtc gaaactccga cagcaccctg
      661 ctgcattgcc tgtcgaacca gcgctttcat gctgtccagc tgcgccccat tcggcgttcg
      721 attttcgtcg cccagaacca ttcgccgtac ctgcgtggcg ccgacgaacg tcgcgaagtt
      781 gatcccgatg ccctgccttt ccagtcgcgc gaaatactgg ctcagggtgc gccactcggg
      841 cgtgatctgg aaatgagcgt acgacacccg gtcggcttga atgatccggt cgttgagcgg
      901 ggcaacggac gttccctcgc cggtgacttc ggtggtgata ccctggaaga tcttggaggg
      961 aacgtgcggg ttcaccagga tagtcagctc ggactgaccc agcatgtcga tgaatccggg
     1021 tgccacgatg agcccgtgcg cgtcgatcac ctttccgctc tttaccgtgc ccagacggcc
     1081 gatcgcggcg atgcgaccgg ccgtaatcgc tacgtcgcca gagtaccagg gagagccagt
     1141 gccatccacg atgcgcccgt ttcggatcag gagatcgaac cggctgggag tggcttgggc
     1201 caacagcgcc ggtgccgccg acagccaggc aacgacggca actgcgcggc agaggtggct
     1261 tgatcggggg cgggcgagcc ggtaatcaga catgagccga atggaaccag gttgaggcga
     1321 gatagtagcc gaaaactgcc gtctcgatca ggcccgctgt caggagtgcg tttcgcgccg
     1381 agcgattggg tgcgaagagc caggacagca gaaagaaaag ggcgaacagc ggcgcgacca
     1441 gaattctcag cgacgcgaag gcagaccagg aattggccat gggtctttgc gagagcacca
     1501 tgggcgccag atagataagg aaagcggcca cgctggtgca cgcgatcgca agcgcggtga
     1561 ttgagaggat cttccagaac gcagggcgcc ctctgagaaa gaagagcgcg gcgccggtgg
     1621 cagggacagc ggccagcccg agggtgccaa ggaacagaag gctatcgcca aagtcgtaca
     1681 tcgcaccgta gacctgcctg tcggggacgt tagtcgctgc gatgtacatg cgaacgacca
     1741 gccaggcgat cgcaaacgcg gccacatatc cgcccgcaac gaggccagct ctagcgaccg
     1801 gtctcatccc gcccgaatag acaatgcgtg gcagaacaca ttgcgcgggt cccatcgtcg
     1861 cttgatccgc tgaaggcggg ggtagtgcgt cggcggttga gttcgcatcg gcggatcaaa
     1921 cacccagctg ctcggccaat tccaggaagt tcgcggcctc gtggtcgaac tggttcttcg
     1981 caggctgggg cggcgctttc ccagctcccc actccagtgg gcgatggatg aaggccgtgc
     2041 ggagccctga ttgacgagcc gcgttcaggt catttgcgtg ggccgccacc atgagcacct
     2101 gatccggccg gacgtcgagc aatcggggca cctgaaggta cgcctcgggg tcgggcttgt
     2161 aatgctgcac cagctcggca gacaggatgc agtcccaggg aaggccggcg ttccgggcca
     2221 tgtcagtgag cagggagaca ttgccattgg aaagcgtcgt gatggtgaat cggcttcgga
     2281 gccgtctcag cccctcgacg gcatccggcc atggcggtag cgcatgccag gcgcggttca
     2341 gctcgtcgcg gtcggcttcg gggaaagcct cgagaccgaa acgaggaatg agcgcatcga
     2401 gcgtgcggcg gtgcagctca tccagcttgg tccagggcag ctcacctctg cgcacccggt
     2461 ccatgcccgg tgagtacgcg ttacgccagg catcggcgaa cgccgcccag tccacgcgga
     2521 gtccatgccg tgctgccagg cgttctccag cagcgatgac gcccgagcgc cagtcaacta
     2581 cagtgccgaa cacgtcgaac gcgacgactc tgtatccaat ctcgagggtc atccgttggt
     2641 gggcctgtgc cgcggcgggc cgtgagctca gtgcagggtc gaggctcgcc gcggtagggt
     2701 aaggatctta cttctcgtct cggatagcgg ctttcacctt cgccatcaat tccggagttg
     2761 cctcccggat cccgtgctgt ttcgcgtgct ccatcgcgtt cttcatgacc tcatcgtcgg
     2821 tctttccgcg gatgatatgc ttgcatccgg atgaagggtc gaccttggca cattctacca
     2881 tcttgcccat aagctgctcc ttctgagcga ttgcctggag attggggaca cggacctgct
     2941 gaacttacct ctcctttaag gcgccggagg aggccgcctg aggcgccggg ggaggccgcc
     3001 ggctagcccc agatgcgcgg cagattcccc gcggcggtcg cctcgagcac gctctgtgtg
     3061 ttcggatcga tggcggtggg cggaacgtga tcgagagcgg cgcgggcaaa gctcggaagg
     3121 ccgaccagca tggccgacac tcgcaccacc cgtccgtcgg caagctcgat gcgaaaccac
     3181 ttggccgact cggagtacgc gagacggcgc acctcgttcc atcgcaaggc gccggtctgc
     3241 ccgatcatcc tgccgtactg aagaccatcc ttagtcagcg tatggcgtcc gttgcgatac
     3301 tccaggatca tcaacagccc gagcgccgcg aaccctacga agaagagagt tatgcccggc
     3361 gacccgctct ttccagggaa cagcgccgag aggagagcga tggtaaggaa gagaccggcg
     3421 cagaccaggc cgatgatgag cgtgctccgg gggtgcacca gggcctggcc gccttgcgtc
     3481 ggtgtcgacg gcagccgacc tcgggccacc caggccatca ccagactcat gatgacgagc
     3541 catacacccc actggatggc cggggcccac cagggtcggt cgctcatgcg gcctgaagcc
     3601 ggctgggcct tcgtgccacg gccaggaggt gggcgctgat gccgaggatc gacgactcgg
     3661 tctcgagctg cccggcgaga cgcagcagat cggagcgctg gcgcgggtcg gcccatcgcg
     3721 cggcgagatc cggcaggagc catcccgggc cctcgagtcc gtagacgccc tcgacatcga
     3781 aaccggcgcc tagcgcctcc gcccgaaggt cctcgggccg gtgaaagtag gcggtggtga
     3841 aatagtcgag ccgctccgtg ggatttcgat gctggccctc ggccagatcg gcttccacaa
     3901 tggatctgaa gcgtggatcc tgaaaaagat cgcggctcat gccgtccagc gcagaggcat
     3961 agcgagaaat gactgcagcg aagagaagac ctccgggctt gagcacacga aacgcttcac
     4021 ccagcgcaag agcccggtcc ggggcgtcgg tcaggtggta gagcgggccg agcagcagga
     4081 cgatatcggc gcaggagtcc gcgaagtcga gccggcgggc gtcgccaacc tggcaagacg
     4141 cgagcggacg gggcagggaa gcggcccggc gacgtgcctc ggcgaccaat cgaggtgatg
     4201 cgtcgaggag atggacagca tagccgactt cggtcagcca catggcatac gccccggcgc
     4261 ctccaccaac gtcaagcacc gttgcgggcg gcgcgggcgc gtggcgttga atcagctcac
     4321 gagtgcggat ctcttcgagc acaaacggga gctgctcgag tcgagtctcc tccggtgcct
     4381 ggtcgtaata ggcggcgatc gaccggtcga agctcggtgg aatctggttc acgggcgatc
     4441 agcggcggta ggccagcagc aggccgtcgc gaatgggtac aaccgacgcc agccaatcct
     4501 ggccggttgt cactagacga gtgaactctc ggacgcccgc cgtcgcctct gaatgatcct
     4561 tcgggtcgat cacctggcca tgccacaaca ggttgtcggc gatgagaacg ccgcccgggc
     4621 gaagcctctc gcgaatgacc ggcagtgcgg ccgggtagcc gtctttgtcg atgtcgagaa
     4681 acacaaggtc gaaagagcct cggacctcac gcaatgtttg gacggcttct ccaacgtgat
     4741 actgaacgat atcggcaaag cccagagtcc cgagacgggt gcgggcctcc ttggagagct
     4801 cagtgtccca caccgtgtga tgaacgacgc cgcccccatt ttcccggacc gcgcgggcaa
     4861 accaggcggt cgaatagccg aatccggagc cgagctcgaa gacggagcgg gccccgatca
     4921 agcgcgagac cgcgtagcag aaatgtccgg cggcagggcc gatgatgggg aacttggctc
     4981 gctccgcctc ggcctccatt gccgcgagct cgggtgctcg cggggggacc agcgagtcga
     5041 gatagcgcct gatttcggga tgaacaggct caagtgtcat cggggccccg gcctgaccac
     5101 cacacccctc acaccgttcg ccgcctgaag gcgctgaggt cggaacgtgg gcgtcagcct
     5161 gtgtggagtc tgccggccac gtcaggtcat tgaattccgg tcgcgtgcga ccagacgacc
     5221 tgccactgct ccccgcgccg ctcatacaca tcagtgtgcc agtaacgttg gcgggggata
     5281 tggcggccct gcacgacgat ctcgagttgc gattgatagc ggatgaccgc cacctcgccg
     5341 tagaccctga cagcgatcgc atcaggttcc cagaccagat actggagctg tcccgaaccg
     5401 atgctgccga ggtattggtc cttcgagagc actccaccaa gggggttgat cagctcgaaa
     5461 tcgtccgtat ggaactggct tgcccgcgtt acgtctcccg agattagggc gcggaggcgc
     5521 tcgcgctccg tggcccggag agtctccgcg gtcgcctgat cgtcaccacc cgggggtcgg
     5581 tctctgccgg tcatggacgc ctctacggac acacaaagat gctcaggtag cgcgaagtcg
     5641 tattagagac gacttgtcca acaaactgac gttgccccat caagctggag gcgttgaggc
     5701 ggtcgagcag gggcctggcc tgagcctcct ctgacggctc atatcgaacc tgaaagccgg
     5761 aaacccgata gcctggcgac gcattgatcc agctggtcag tgctctcgtt cgtacggttg
     5821 cctcaccccg caggcgagtg gcgatcgagt cggccaaagg ctttgctgcc gggccggagc
     5881 tctcacagta aaagacgtcg tatcgccatt tcgaggtccc accgggtgtc ctaagtctct
     5941 cgttatccaa tttgaattgc tgttcctggg ccagtttctc tgccgcggcg acgcgaacag
     6001 gggcggtccc gcctcgttgc aatgcgtctc gcaaggcgtc gcgcaaggtg gaatcattca
     6061 ttctcgctac cagactcaac gcagcgtact gctgtgccga atccttactg ctgaccgagg
     6121 ccatgacgac tttgaatgtc tccatgtcgt actcgcggtc ggcgcgcttg gcgagttgct
     6181 gggcatccag ctccgccttg cgactgctca ccttccattg aacgaccgag aaaaagattg
     6241 tggcaatgga aatgagcgcg gtcgccagcg tggcaaagtt ccgtctcacg aaacgatctt
     6301 cggatgccgc tttgcgctct tccaacgcaa ctcttcgttc ctcgaccgcc actcgccgtt
     6361 cgtccaccgg aaggcgcttg ggttcgagtt cctggttgct cataacgtgt gctcgatcag
     6421 cagctgggaa ggtggcgcct aagtagctcc tgccaccgct cctcgttcat ggcgatgccc
     6481 tgccgtttga gcccgtgctc ctcgagccat ttgggttctg ctgccgctgg agcttccggg
     6541 cgaccttcac tccacggatg gtggcccgaa ggctgccgac gaccagcact gccaggcaga
     6601 tcgtgacgta gcccagctcc ggtaaaccac gcagaaactg gagccccaac gcaaccacga
     6661 ggagtaccgc acccagacgg acgaggtcat cgccgcacac ggcgttcgcc tcgtaccaga
     6721 tcctcgggtc ggcgaatgta gcgggcacgc gcagcccgta ccatcggttg ggcggcaccc
     6781 accggtgcgc gagaggccat cccacgatga gcgtggcgag cccgagcgcc ggaaatgcta
     6841 ttcgcgatac gatcacgacc gatggggcta ttcagctacc ccccacctaa cgagcagatg
     6901 aagccggctg cgcggcagct gagcggcgca gcatccaccc gtacaaccat acgatcaccc
     6961 ccacaaagcc gatcagccca agccagggcg cgggcccgaa ggtctcgggc acgaatgcga
     7021 tcggcgcctc cttcgacagg ccgacaatca agccggcatt gatgacgccc cagaggctct
     7081 cgccgacaat cagtcccgat gctaccagtg tgcccagtcg atcagcgcgc tcggggttgg
     7141 gcgactggcg agcgcggcgg tcgtaccaat gggagagcac cgcgccaacg accacggcaa
     7201 aggtggccga catggggaga tagatcccga tgccgaccgc aaggggcgga atgcgcagtt
     7261 tcttcatcgc cccgagggtg gcgtcgagca ggatgagtcc tacgccaatc agcccgccga
     7321 tgccgatcat cttccattcg agttttccgc cgatcacgcc ttgcgcgagt gccgagatga
     7381 gcgtggcctg gggggctgga agcggattgg gggcgacgac gccgacgttc gcggcgccgg
     7441 cgaatccgta ggccttggcg agcaagttaa gtacgaacgg gatgaccgcc gctccggcgc
     7501 cgaccccgac gatgagcgcg atctgctgcc gcatgggaga ggctcccacc agctgcccgg
     7561 tcttgagatc ctgcaggttg tcgttcgaga tcgtcgcgca ggcaaagacg atggccgtca
     7621 cgaagagtgc gaacgcgacc agtgccggcc tcgtgtcctg agtcggcgac acagtgagcg
     7681 cgagcaccga tgcgcagacg acgatcgaca ggatgccgac gccggagatc gggctgttcg
     7741 atgcgccgat gagtcccgcc atgtaaccgc agatcccggc aatgatgaac ccgacgatca
     7801 gcacgaacgg caccgcgacc agggtgagcg ccagcgcgct cgacgccagc acggtcgact
     7861 tcgcgaatgt gaacgccagc caggcagaga tgacgaggca cgcgacggtg agcccgatga
     7921 tccaggaggg cgagagatcg cgatcgcgat catccgccac cttcttggcg cgcgaggcag
     7981 caagggtgct tacgagtccg ccgacgaccg gcttggcgag cgtcgccagc gtatagatcg
     8041 cggcgaccgc gatcgtgccg gcgccgatga accgcacttg ggtgcgccag atggtaaggg
     8101 tgtgtgccgc gagcgttgtg tcggcggcgg cgggttgcat cgacgtcagg atcgggacgg
     8161 cgatggccca cgagatcacc aggccggtca gcatggccat gccgaccgag aggccgacca
     8221 ggtggcccgc gcccaggagc gcgagcgacc acgcgatgtt gtagccactc gaggccgtgg
     8281 cgcccaggcg aaagaagccc acaacttctg ccgcggcgat gcgggtaccg gtgatgatgg
     8341 ccaggcctgc cgacgcgacg gagcccagca tcacagccac gagcccttct ctcgcctcgc
     8401 cggtctcgtc cttggtctcg ccccgggtcc ccgacccgac ctttagtacc tcggcggcgg
     8461 caacgccttc ggggtagggg aggtcggaag tggtgacgag agcgcgccgg agcgggatcg
     8521 tgaagaggac cccgagcacg ccgccgctca cgcaaatgag aaacgattgc cagaatggga
     8581 atccggtcca ccagcccacg atcacgagtc ctggcaacac gaagatgatt gcggagagcg
     8641 tgccggcggc ggacgcgacg gtctgaacga tgttgttctc gaggatcgac gagtccttga
     8701 aggcgctcag gatcgccatc gagatcacgg ccgcggggat ggacgacgcg acggtaagtc
     8761 cgaccttgag gccgaggtaa atgttggcgg cggtgaacgc agtagtgatg agggccccga
     8821 ggaggaggcc ccgcagcgta agctctcttg gtcgcatggg tggcaattct acaggtttcc
     8881 ggaggttctg acgagtgctg atggggtcat cgcgcgtacc tcactcattg gggcgatttg
     8941 gctgctgggc gcacagcgga tcagagttgg caatcaccgc cgccagggcg atgaggaagg
     9001 ggcgagccat ggcaaacccc gggttaggtc aaaaccgctt aggcactgac cacgggtagg
     9061 accgtcgttg ggaggcttgg aatctggagt ggggcaggga ccagcgccag cgtggcaacc
     9121 attcaccccg cgccccggtg cgggtaacag attcccgcac cggcgcggca gtgataccta
     9181 cttcagcggc ttgccctcgt tcgccatctc caccgcgacc gccgcccc
//
LOCUS       DASRJQ010000563         3961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3750889, whole genome shotgun sequence.
ACCESSION   DASRJQ010000563 DASRJQ010000000
VERSION     DASRJQ010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3750889"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="VFB89_12845"
     CDS             <1..816
                     /locus_tag="VFB89_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HZM28229.1"
                     /translation="KIEKDTALDNLRPILQASDAIMVARGDLGVELPFEKVPLAQKRV
                     IREANLHGKPVITATQMLESMVHAPRPTRAEASDVANAILDGTDAVMLSAETAVGEFP
                     LEAVEAMARIAREIEQQRRGRSPAFDMAVGRRREELERLQKGESGTFTPTRTEDAIAV
                     AVCAAADLMSAPLVVCFTSSGFTARTVASYRPSVPIFAITPEPETFRQLSLVWGVVPA
                     LAEHLPNYDSMLAVARHKLLELKLAEPGERVVVTAGVPFDQPGTTNLLKIEAV"
     gene            820..1596
                     /locus_tag="VFB89_12850"
     CDS             820..1596
                     /locus_tag="VFB89_12850"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZM28230.1"
                     /translation="MRLTFLGTGTSFGVPQIGCDCAVCRSSDARDRRSRAGALVQVSD
                     TAILIDTPPELRLQLVAAGQPRIDAVLYTHAHADHVNGIDDLRIFSVKQRRPLPLYGP
                     AETLETLRASFSYIFDDNVRPYEGTSKPTLELRPVEPGRPIVIAGIPVLPLAFQHGHL
                     RVFGYRLGSLAYITDAKEVPDSARACLGDLKVLALNALWWRPHPTHLSIGEAVRTAQE
                     LGAERTFLTHLTHETAHADLVAQLPPGIEPAYDGLAVEVD"
     gene            1596..2105
                     /locus_tag="VFB89_12855"
     CDS             1596..2105
                     /locus_tag="VFB89_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28231.1"
                     /translation="MKLRGAGFLVVWVMGLGLLVGCGADSPSQRPLGSIPTDDLIYNN
                     ERAGFTLALPPAWHDRYDVDARSGPIAAAQWPYASDAVAFTYKPVEPDQPQPTLLTIL
                     VYRREDWNSIAAESGKPHGILVAQQADDVYIASVADSNPFAGGSRDADHFNAMRLTID
                     QVKDALTLH"
     gene            2216..3631
                     /locus_tag="VFB89_12860"
     CDS             2216..3631
                     /locus_tag="VFB89_12860"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HZM28232.1"
                     /translation="MTDVIKDTVGLFGGAVALAGSGVTVRDDAALRSAATDRLVWRAV
                     FGSSEEQEAARWLLWELGQSSGARPASINDLYMARGRGECSGFTVPAINVRAMAYDTA
                     RAVFRAAEKGKVGALILEIARSEIAYTEQRPAEYVAVMLAAALREGYTLPLFIQGDHC
                     QVNQKKYQADPEAEVGEVKKLIAEEVGAGFYNVDVDTSTLVDLSKTSLTEQQRLNYER
                     AAEITRFIRDREPQGVTISVGAEIGEVGGHNSTVEELHAFMEGYNAAVAAGRGGDGIS
                     KISVQTGTSHGGVVLPDGSIAEVKLDLDALAALSREARTKYGLAGAVQHGASTLPADA
                     FGNFPRIETCEIHLATNFQNIIFDHPKLPEDLRNAIKTWLAANAQGERKAGDSEEQFF
                     YKARKKAIGPFKRELWSLPEGVRAAIAADLESTFAFLFAQLKVNGTADLVRRHVKAPL
                     QHHAALKPALVTAEDDADAGE"
     gene            3640..>3961
                     /locus_tag="VFB89_12865"
     CDS             3640..>3961
                     /locus_tag="VFB89_12865"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HZM28233.1"
                     /translation="MRHRPKPEVVYVERSGDSSVKWLLWGALLGAGLALLYAPRSGEE
                     TRRSVQRKLRTLRAMAEEKLDELAQQFGGRGGSLEDLDDAASDDEPLPVRPEPPRTAS
                     ARQEL"
BASE COUNT          727 a         1256 c         1283 g          695 t
ORIGIN      
        1 aagatcgaga aggacacggc gctcgacaat ctccgtccga ttctgcaggc ctccgacgcc
       61 atcatggtgg cgcgaggaga tctcggagtc gagcttccct tcgagaaggt gcccctcgcg
      121 cagaagcgag tgattcgtga ggccaacctg cacggcaagc cggtgatcac cgcgacccag
      181 atgctggaat cgatggtgca cgcaccccgg cccacccgcg cggaggcgtc ggatgttgcc
      241 aacgcgatcc tcgatgggac cgatgcggtc atgctctcag ctgaaacagc ggtcggggag
      301 ttccccctcg aggcggttga ggcgatggcg cggattgctc gcgaaatcga gcagcagcgc
      361 cgggggcgga gtccggcgtt cgacatggca gtcggccgcc gtcgggaaga gctcgagcgc
      421 ctgcagaaag gcgagtcggg caccttcacc cccacacgca ccgaagacgc aatcgccgtt
      481 gccgtctgcg cggcggctga cctgatgagc gccccgctcg tggtctgttt taccagcagt
      541 ggcttcaccg cccggacggt cgcctcgtat cgcccttccg tccctatctt cgcgatcacg
      601 cccgagcccg aaacgttccg ccagttgtct ctggtctggg gtgtggtacc cgctctggcg
      661 gagcaccttc ccaactacga ctcaatgctc gcggttgccc gccacaaact gctcgagctc
      721 aagttggccg agccgggtga gcgtgtggtg gtaacggcgg gcgtgccgtt cgatcaaccc
      781 ggcactacca acctcctcaa gatcgaagca gtttaggaga tgcggctcac ctttcttggc
      841 accggtacgt cgtttggcgt gcctcagatc ggttgtgact gcgcggtgtg ccgttcttcc
      901 gacgccagag atcgccgttc tcgtgccggc gccctcgtgc aagtgagcga cacggcgatt
      961 ctgatcgata ccccgcccga gctgcgcctt cagctagtcg ccgccgggca accgcgaatc
     1021 gatgcggtcc tctataccca cgctcatgcc gaccacgtca acggcatcga tgacctgcga
     1081 atattctcgg tgaagcaacg tcgcccgcta ccgttgtacg ggcccgctga aacactcgaa
     1141 accttgcgag catcgttcag ttacatcttc gatgacaacg tgcggcccta cgaaggaacc
     1201 tcgaagccca cgctcgagct acggccggtc gagcccggcc ggccgatcgt catcgcgggc
     1261 ataccagtcc ttccgctggc gtttcagcac ggacatctgc gggtgttcgg ctaccggctc
     1321 ggcagtctgg cgtacatcac cgatgccaag gaggtacccg actcggcccg cgcctgcctg
     1381 ggtgatctga aagtgttggc actgaacgcg ctttggtggc ggccccatcc cactcacctg
     1441 agcatcggcg aagcggttcg tacggctcag gagctgggtg ccgaacgaac cttcctgacc
     1501 cacctcaccc atgaaaccgc acacgcagac ctggtggcgc agcttcctcc cggaatcgag
     1561 ccggcgtatg acggtctcgc cgtggaggtc gattgatgaa gttgcgcggc gctggctttc
     1621 tcgtggtttg ggtgatgggc ttgggtctgc ttgtgggttg tggcgctgac tcgccttcgc
     1681 agcgtccgct gggctccatt ccaaccgacg atctgatcta caataacgag cgggcggggt
     1741 tcacgctcgc gttaccgccc gcctggcacg atcgctacga tgtcgacgcc cgctcgggtc
     1801 caatcgcggc ggctcagtgg ccttacgcca gcgatgccgt tgcgttcacc tataagccgg
     1861 tcgagccgga ccagccacaa cccacactcc taacgatcct ggtgtatcgg cgggaagact
     1921 ggaattcgat tgccgcggag tccggcaagc cgcacggcat actggtggcc cagcaggcgg
     1981 atgacgtcta catcgcgagc gtagccgatt caaatccgtt tgcagggggt tctcgcgacg
     2041 cagaccattt caacgccatg cggttgacca tcgatcaggt aaaggacgcg ctcacgcttc
     2101 actaaggccc cgtcctccaa aggcgtcgct cctgtttgac caccctcggc gctcgcccta
     2161 cattcttggt ttcggcgcaa gctgacgatc ccgctcggct caactgggac gctcgatgac
     2221 agacgtgatc aaagacacag tgggactttt cggcggagcg gtcgcgttgg ccgggagcgg
     2281 cgtcacagta cgcgatgacg ctgccctgcg atccgccgcg accgaccggc tggtgtggcg
     2341 agctgttttc ggatcgagcg aagaacagga agcggcgcgt tggctgctct gggagctggg
     2401 acagagcagc ggcgcacgcc ccgcttcgat caacgatctc tacatggccc gcggccgcgg
     2461 ggaatgcagc ggattcacag tgccggctat caacgtgcgc gccatggcgt acgacaccgc
     2521 ccgcgcggta ttccgcgcgg ccgaaaaggg caaggtcggc gcgctgatcc tcgaaatcgc
     2581 acggtcggag atcgcctaca ccgagcagcg gccggctgaa tacgtcgccg tgatgttggc
     2641 ggctgcgctt cgcgaaggct acacgcttcc gctcttcatt cagggcgacc actgccaggt
     2701 caaccagaag aagtatcagg ccgatcccga agcggaagtg ggtgaagtca agaagctcat
     2761 cgccgaggag gtcggtgccg gcttctacaa cgtcgacgtg gacacgtcga ctctggtgga
     2821 cctcagcaag accagcctga ccgagcagca gcggctcaac tacgagcgcg ccgccgagat
     2881 cacccggttc attcgcgacc gtgagccgca gggggtcacg atttcggtcg gcgcggagat
     2941 cggcgaggtg ggcggccata acagcaccgt cgaagagctg cacgcgttca tggaggggta
     3001 caacgcagcg gtcgcggctg gtcgaggcgg cgacggaatc agcaagatct cggtgcagac
     3061 ggggacttcg cacggtggtg tggttctccc cgacggcagc atcgcggagg tcaagctgga
     3121 tctcgatgcg ctggcggctt tgagccgcga ggcccgaacg aagtacggtc ttgccggcgc
     3181 agttcagcac ggcgcgagca ccttgccggc agatgcgttc ggcaacttcc cgcggatcga
     3241 aacctgcgag atccatctcg ccacgaactt tcaaaacatc atcttcgacc accccaagct
     3301 tcccgaagac ctgcgcaacg cgatcaagac ctggcttgcc gcgaatgcgc aaggggaacg
     3361 gaaggcgggg gattcggaag agcagttctt ttacaaggcc cgaaagaagg caatcgggcc
     3421 cttcaagcgc gagctgtgga gtttaccgga gggcgtgcgt gctgcgatcg cggccgatct
     3481 cgagagcacg ttcgctttcc tgtttgcgca gctcaaggtc aacggcacgg cggacctggt
     3541 ccggcggcac gtcaaggcgc cgcttcagca ccatgccgcg ctcaagcccg cgctggtaac
     3601 cgcggaagac gatgcggatg ccggtgaata ggagactcca tgcgtcatag gccgaagccc
     3661 gaggtcgtgt acgtcgaacg gagcggcgac tcgtccgtga aatggttgct ctggggcgcg
     3721 ctgctgggag caggacttgc cctgctgtac gcgccgcgaa gcggagagga aacccggcgc
     3781 agcgtacagc gaaagctccg aacgttgcgg gcgatggccg aggagaagct tgatgagctg
     3841 gcccagcagt tcggcggccg gggcggatcg ctggaagacc tcgacgatgc tgcgtccgac
     3901 gatgagccgc tgccggtgag gccggagcct ccccggacgg cctcggcgcg gcaggagctc
     3961 g
//
LOCUS       DASRJQ010000564         6511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1798980, whole genome shotgun sequence.
ACCESSION   DASRJQ010000564 DASRJQ010000000
VERSION     DASRJQ010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6511
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1798980"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VFB89_12870"
     CDS             <1..264
                     /locus_tag="VFB89_12870"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HZM28234.1"
                     /translation="ATNRLPTVAQSSVWPQFGGLLSYGPDNADVFRRGAALAAKILKG
                     AKPADLPVEQANKFELIINLKPAKALGLTIPPSLLARADQVIE"
     gene            344..961
                     /locus_tag="VFB89_12875"
     CDS             344..961
                     /locus_tag="VFB89_12875"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZM28235.1"
                     /translation="MSRPETRYAKSGDVHIAYQVTGSGPRDIVHVPGFVSHLELGWDV
                     PALAHMFERLGRFSRLIRFDKRGTGMSDRVQVGTLEERMDDVRAVMDAAGSEKAALFG
                     RSEGGPMSLLFAATYPARTTALVLYAAFARRLWSPDYPIGVPEIDRQAYLDLIARQWG
                     EGADLSIVAPSLAHDPEASRRFAMSRRMSAKPRRSARHCAHEQRD"
     gene            945..1676
                     /locus_tag="VFB89_12880"
     CDS             945..1676
                     /locus_tag="VFB89_12880"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZM28236.1"
                     /translation="MNSEIDVRHVLPVIRVPTLILHRATDRDVAVENGRYLAEHIPGA
                     RYVEFAKGDHYPTASADYDPEIDEVEEFLTGVRHGPAPDRILATVLFTDIVGSTERVV
                     ALGDRRWRELKADHHKIVRDELARFRGREVDTAGDGFFATFDGPARAIRCASAIQLAV
                     RRLGIDIRAGVHTGEVEVAHDEVTGIAVHIGARLMGMAGPGEIVVTSTVKDLVAGSGI
                     EFGDRGVHVLKGMPTPWNVFAVAGV"
     gene            complement(2029..2955)
                     /gene="istB"
                     /locus_tag="VFB89_12885"
     CDS             complement(2029..2955)
                     /gene="istB"
                     /locus_tag="VFB89_12885"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HZM28237.1"
                     /translation="MTAAPAIDLDALLRRLHLPTVRRLYPDLATRAESEDMAYRDYLA
                     VLMAEEVAHRAQTRIQRCVRRARFPFLKTIDEYDFTFQSAVRLSLLGSALGPEFVTGG
                     HSLVLSGPSGTGKTHLAVAVAYRAIQNGFDAAFTTATALIAELSLASRRGRLRHTLTA
                     YTHPHVLVIDEVGYLSYGPDAANVLFQVVNDRYLHQRPLIFTTNKPLAAWGRVLHDPD
                     LAEAILDRVLERGRHIELRGRSYRTRHAPLDLNPGPEAPSPEPARVSGNHPPEFPERA
                     SASRVSVSSRIEHVVGSKDIRRHLSAMRFSAN"
     gene            complement(2952..4337)
                     /gene="istA"
                     /locus_tag="VFB89_12890"
     CDS             complement(2952..4337)
                     /gene="istA"
                     /locus_tag="VFB89_12890"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HZM28238.1"
                     /translation="MIDAMTRHEIQVLRAAGIAEQVVAQRTAVSVRSVERISQEPPVT
                     GPIRTVRVGRPALTAAWADRVVGWLTEDRALPGMEILRRAHEAGYAGGKSALYELIRR
                     LRPTPTVPVVRFEGVPGEFSQHDFGQTDVRYVDGRTERVHFFASRLKWSRAVDVRLVP
                     NEQEEALVRALLRSFEAFGGIPLVTVWDNPKTVVQARQGDLIVWNAVFGQVALDYRFA
                     PELCWPRAAQQKGAVEQLVGWVKRSFFTCRRFHDRADLERQLTAWLLDVNTVRPSRAT
                     GIIPAVRLAEERARLRPLPIAPRAYALKIPVVVSAQGRVRHAGVDYSMPADTLGQTAT
                     LHLYEDRLEIVTKTGAVVKHPRRARGASILPEHRAALLDRVHGARGQLYFQRQSLWEL
                     GPPAEAWLTELVHRRPARWRPDVEACFALLQDYGPTALQDAFAWGVRHGAIGAEYVRT
                     RLATRATEVSA"
     gene            4927..5805
                     /locus_tag="VFB89_12895"
     CDS             4927..5805
                     /locus_tag="VFB89_12895"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZM28239.1"
                     /translation="MGTIRFALGQSIVPVVAHVPGERVLRCLGTAFFISCTGLLVTAA
                     HVITDPIERRYGDVRELEDRTWHLGELKLGVMIPLNPITQGQGYIFRELEWAGFLGAK
                     SEHPLPIAGVSLKLTSDTAICKIPPIAPGIPYQPLAIVQSGVVGIGMAVGKTATAIGY
                     AGMRDVELSQESADVVSGEFAFDLHVSTGTILERFPDNMTTRAVPTPGACFAASLKLP
                     GGMSGSPIFDDEMIYVHGVVSKGWVDADGLAPFGYGCMLSHSLGLPIRPLGDKTLLDL
                     HESHDHGMPRLSGPAI"
BASE COUNT         1137 a         2021 c         2193 g         1160 t
ORIGIN      
        1 gccacgaacc ggctccctac ggttgcgcag tcttcggtct ggccgcagtt tggcggcctc
       61 ttgagctacg ggccggataa tgccgatgtg ttccggcggg gcgcggcgct ggcggcgaag
      121 attctcaagg gtgccaagcc agccgatctg ccagtggagc aagcgaacaa gttcgagctg
      181 atcatcaacc tcaagcccgc gaaggccctc ggcctcacga tcccgccgtc gctgctggcg
      241 cgggcggatc aggtaatcga atgatggacc gccgccgccg cctcctgcta accccgctcg
      301 ctgctctcct acccacgatg cttcttgccc aaggcgcact atgatgtccc gacctgagac
      361 gcgatatgcg aagagtgggg atgtccacat cgcctatcaa gtcaccggct cgggtcctcg
      421 cgacattgtt cacgtccccg ggttcgtgtc tcatctcgaa ctcggctggg acgtccctgc
      481 actcgcccac atgtttgaac gtctcggtag gttttcgcgc ctgattcgct tcgacaaacg
      541 cggtaccggc atgtccgatc gcgtgcaggt gggcacgttg gaggagcgga tggacgacgt
      601 ccgggccgtc atggacgctg ctggatcgga gaaagcagcc cttttcggcc gctccgaagg
      661 cggcccgatg agtcttctct tcgctgcaac ctaccccgcg cgcacgaccg cgctcgtgct
      721 ttatgcggcc ttcgccaggc gactgtggtc gcctgactat cccattggag tcccggagat
      781 cgaccggcag gcgtacctgg acctgattgc gcggcagtgg ggagaggggg ccgacttgag
      841 catcgtcgca cccagtctcg cccacgatcc ggaggccagc cgccgcttcg cgatgtcgcg
      901 gcgcatgagc gccaagcccc ggcgcagcgc tcgccattgc gcgcatgaac agcgagattg
      961 atgttcggca cgtccttccc gtgatccggg tgcccacgct gatcctgcac cgagcgaccg
     1021 atcgcgatgt agccgtcgaa aacggccgct atctcgctga gcacatcccg ggcgcccgat
     1081 atgtcgagtt cgcaaaaggc gaccactatc cgacggcgag tgccgactac gatccggaga
     1141 tcgacgaggt cgaggagttt ctcacgggtg tccggcatgg tcctgcaccc gaccgcatac
     1201 ttgccaccgt tctcttcacg gacatcgtcg gatccaccga acgcgtcgtg gcattgggcg
     1261 atcgacgatg gcgggagttg aaggcggatc accacaagat cgtgcgagac gaactcgcgc
     1321 gctttcgtgg gcgcgaagtc gacaccgccg gcgacgggtt ctttgcgacg ttcgatgggc
     1381 cagccagggc gattcggtgc gccagcgcga tccaactcgc agtgcgacgc ctcggcatcg
     1441 acattcgcgc aggtgtgcac actggcgaag tcgaggttgc ccacgatgaa gtcactggga
     1501 ttgcggtcca tatcggcgcg cgcttgatgg ggatggctgg gccaggtgag atcgttgtca
     1561 caagtactgt gaaggatcta gtcgccggtt cagggatcga gtttggcgat cgcggggttc
     1621 acgtcctcaa aggtatgccg acgccgtgga atgtgtttgc tgttgcggga gtgtgaggga
     1681 cgccgcctgg ccgctagcat cgagacagaa tcgcgggaga gcgcgtggcc gttggctgca
     1741 ccggcagggc tgggacctcc agcttacgca gtacggtgac ggtcattggc gagcgacctt
     1801 ctgcatgacc ggtcaggccc actcaatcct tggtggctca gcatgggagc ccacggtgtg
     1861 gcgggccgtg cagcgggcgg ggtgggatgc gttgcagcac gctgagcggc atctctgaga
     1921 ttaggagccg caggagggag gagaagccat gacgcgagaa acagccaatc tgtgggctga
     1981 ggctcatgcc gcatttgagg gacaccgtct accggtcgac gggaatgctc aattggcgct
     2041 aaaccgcatc gcgctcaagt gtctccggat gtcttttgat ccgacaacat gttcgattcg
     2101 tgaagagacg ctcacgcgtg aagcacttgc acgttccgga aactctggcg ggtgatttcc
     2161 ggaaactctg gcgggttcgg gcgatggtgc ctctgggccg gggttgaggt caagcggcgc
     2221 gtggcgggta cggtaggagc gtccacgcag ctcgatatgc cggccgcgct cgagcacgcg
     2281 gtcgaggatg gcctcggcga gatccgggtc gtgcagcaca cggccccagg ccgccagcgg
     2341 cttgttggtg gtgaagatca gggggcgttg gtggaggtac cggtcgttca cgacttggaa
     2401 gaggacattg gcggcgtccg ggccatagct gagatagccg acctcgtcga tgacgagcac
     2461 gtggggatga gtgtaggccg tcagcgtgtg ccggagacgg ccccggcgag aggccagcga
     2521 gagctcggcg atgagcgccg tcgccgtggt gaaggcggca tcgaagccat tctgaatcgc
     2581 gcggtaggcg accgcgaccg ccaggtgcgt cttgccggtg ccgctgggcc cgctgaggac
     2641 cagcgagtgg ccgccggtga cgaactcggg gccgagggca ctgccgagca gcgagagccg
     2701 gaccgcggac tggaaggtga agtcgtattc gtcgatggtc ttgagaaatg gaaagcgcgc
     2761 gcggcggaca cagcgctgga tgcgcgtctg ggcccgatgg gcgacctcct cggccatgag
     2821 gacggccagg taatcgcggt acgccatatc ctcgctctcg gcccgcgtgg cgagatccgg
     2881 atagaggcgc cggacggtcg gcagatgcaa gcggcgcagg agcgcgtcga ggtcgatcgc
     2941 cggggcggcg gtcacgcgct gacctctgtc gcccgcgtcg ccagacgggt gcggacatac
     3001 tcggcgccga tggcgccgtg gcggacgccc caggcgaagg cgtcctgcag cgcggtgggc
     3061 ccgtagtctt gcagcagcgc gaagcaggcc tcgacgtcgg gccgccaccg cgcgggccgg
     3121 cggtgcacca gctccgtgag ccacgcctcg gccgggggcc cgagctccca gagactctgg
     3181 cgctggaagt agagttgccc ccgcgcgcca tgcacgcggt cgagcagcgc ggcgcgatgc
     3241 tccggaagaa tactggcgcc acgcgcgcgc cgcggatgct tcaccaccgc gcccgtcttc
     3301 gtgacgatct cgagccggtc ctcgtagaga tgcagcgtcg ccgtctggcc gagcgtgtcg
     3361 gccggcatcg agtagtccac gccggcgtgg cggacgcggc cctgggcgga gacgacgacc
     3421 gggatcttca gggcgtacgc ccggggcgcg ataggcagcg ggcgcaggcg agcgcgctcc
     3481 tcggccaagc ggacggccgg gatgatgccg gtggcgcggc tgggccgcac ggtgttcacg
     3541 tcgagaagcc aggccgtgag ctggcgctcg agatcagcac gatcgtggaa gcgccggcag
     3601 gtgaagaagc tgcgcttgac ccagccgacg agctgttcga cggcgccttt ttgctgggcc
     3661 gcccggggcc agcacagctc gggggcgaag cggtagtcca gcgccacttg gccgaagacg
     3721 gcgttccaga cgatcaggtc gccctgccgt gcctgcacga cggtcttggg attgtcccaa
     3781 acggtgacca aggggatgcc gccgaaggcc tcaaagcttc ggagcagagc gcgcaccagc
     3841 gcttcttcct gctcattggg caccagccgc acgtcgacgg cccggctcca cttcaagcga
     3901 ctggcgaaga agtgcacgcg ctccgtgcgc ccatcgacgt agcggacgtc ggtctggccg
     3961 aagtcgtgct ggctgaactc gcccggcacg ccctcgaagc gcaccaccgg gacggtcggc
     4021 gtcgggcgca gccggcggat caactcgtag agggcgctct tgcctccggc gtaccccgcc
     4081 tcgtgcgcgc ggcgcaggat ctccatgccc ggcagggccc gatcctcggt cagccagccg
     4141 accacccgat cggcccacgc ggccgtgagg gccggccgcc cgacccggac cgtccggatc
     4201 ggaccggtca caggcggctc ctggctgatc cgctccacgc tgcgaaccga cacggcggtc
     4261 cgctgggcaa cgacctgttc cgcaatccct gccgctcgga gcacttggat ctcatgccgg
     4321 gtcatggcgt cgatcactcc tctcccttcc cgaggcggcg cgtaccggcc gccgacctcg
     4381 agaggagcgt gggctcagag ccctggatga cccgccagaa tttccggaat ccgacccgtc
     4441 agaatttccg gaacgcgcag cactcagtca gctaattact ttcgacgacc gcgatgccaa
     4501 tccgcatcgc gaggtggagc ctatcgttgt tctttcgttc aatggtaaga ggtatgtcat
     4561 tgacggtaga agaagagtca acaagtggtg cgcttctctg ggagcgcagc tgcggcgcgc
     4621 cgtcatcatc gaaccgacac ctgccttcta cgcgcaatgg gccgtccccc catcttaagt
     4681 ctgtcgcggt ttcctgttgc tgccgcaggg tgacgcagta acaacagtcc agttcgctcg
     4741 gtctccctgg agattctcgg gttagagtgg tcgcgctttg gagattctaa aactctattt
     4801 cggaaagtcc gacaagggta tctgggcgat ccagggcatg aaatcaatcc ctttgaagcc
     4861 ccgggggacc gaagcggccg atttcttccc gattgaggac gacctcgttt tggttcagcc
     4921 ccgttcatgg ggactatccg gttcgcccta gggcagagca ttgttccggt cgttgcgcat
     4981 gtccccggcg agcgtgtcct ccggtgtctc ggcacagcat tcttcataag ctgcaccggc
     5041 ttgttggtca ctgcggccca cgtaatcact gatccgatcg agagacggta cggcgacgtt
     5101 cgcgagcttg aagataggac atggcacctc ggcgagctga agcttggtgt aatgattccc
     5161 ttgaacccga taactcaggg tcagggatac atctttagag aactagaatg ggcgggcttc
     5221 ctaggtgcga aatcggagca cccgctgcca atagccggcg tcagcctcaa actaacttca
     5281 gacactgcca tctgcaaaat accgccgatt gcacctggca tcccgtatca accgctcgcg
     5341 atcgtgcagt ccggggtcgt cggcatcgga atggcggttg ggaagacggc tactgcaatc
     5401 ggatatgctg gaatgcggga cgtagagttg tcgcaggagt cagcggacgt tgtgtctggc
     5461 gaattcgcat tcgacctcca tgtgtcgacc ggaacaatcc tcgaacggtt tccagacaac
     5521 atgaccacga gggctgttcc tactcccgga gcatgcttcg ccgcatcgct gaaattgccg
     5581 ggaggtatga gcggcagccc aatctttgat gatgagatga tctacgtcca tggggtggtc
     5641 agcaaaggtt gggtggatgc ggacggattg gccccgtttg gttacgggtg tatgttgtcg
     5701 cactcgcttg ggttaccgat cagaccactc ggcgacaaaa cactgctgga tcttcatgag
     5761 tctcatgatc acggcatgcc tcgactcagc ggtccagcca tctagaaacg ccctgcgcgc
     5821 ccgccccgga acttgcggcg caaacttatt tagcgaatgc cggcgttctt ggaccctcgc
     5881 gcgcgcgtag ccgccccgcg cggcactcag ccgcatctgc gatcaggcaa acggcgggcc
     5941 tgggggtgcc ctgggagtgg gatacggccg gacaacggag agcgtagggt gtgtgccgtg
     6001 tatatagcga cgcccggccc ctaacgacca cttgtggcct tgggggtggg tggtccaatc
     6061 ggccctcacc catcttccgc cgcctgagag caggaccagg gccggcactc caagttcagt
     6121 ccaagttggg ggtctcaact cgtcgtactc ggtgtgaccc gggatctgga agccacgagg
     6181 agagaccgcc ggcaaatgcg tgatgcggct gtgctcttcg gtgctcagtg cggatggact
     6241 gcgacggagc gatggagggc cagccccatt ttcaagtcca ctgccttgcc agttgaggct
     6301 agccctccgt gcgaacagga tcgcacactt ccgaggcgcg ggccgatggc aggagccctc
     6361 gcctcagcgg gcgaccagca gctcgacgag ctctgcgact tcctgcagca cgtccacggt
     6421 caccggatag gagatcgcgc ggcgcagggg gaaacgccga cgctccgccc gcggcgcctc
     6481 gcgcggccgt gcgaggaagt tcgtcgcgac c
//
LOCUS       DASRJQ010000565         5228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_4033951, whole genome shotgun sequence.
ACCESSION   DASRJQ010000565 DASRJQ010000000
VERSION     DASRJQ010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4033951"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="VFB89_12900"
     CDS             complement(<1..695)
                     /locus_tag="VFB89_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HZM28240.1"
                     /translation="MAERLTLPVLPLREVVLFPGVTAPIGAGRPGTLRAIEAALTTPE
                     RQVFAVSQRQNVETVTSEVLYTVGTIARIGQMQRGLSGMQLLLHGEQRGIALHYTEKD
                     GYLQAVVREAEEMLPLRPDETAFVALHREARARAAELGQKSGLPEEVVQQVLAGVNEP
                     GKFADLVAGYIDITPQQRQALLETLSVEERLRRVLVHVQRQISMVDAQEDIKSQVQEE
                     LGERQREMFLREQL"
     gene            1050..2942
                     /locus_tag="VFB89_12905"
     CDS             1050..2942
                     /locus_tag="VFB89_12905"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM28241.1"
                     /translation="MPLIGDSTGDSTNHPLPTRLSRALGDMYTLEGEIGRGGMGVVYR
                     ARDERLQRRVAIKVLPPELAFQNDIRARFTREAQTAARLSHPHIVPIHTVGEGEGLVY
                     FVMGYVDGESVAGRIRRRGKLPVDEARRIMAETADALGAAHTFGVIHRDIKPDNILLE
                     GTRGRVMVTDFGIAKALSQASGATLTGAGVAIGTPSFMSPEQAAGEREIDGRSDIYSL
                     GVVAYQMLTGDLPFYAPTVAGILMKQITEPAPDVRTKRPDIPEDLALAVSRCLEKDPQ
                     NRWPTADALRRGLENRSVGGYRPTGTGWRTGQAAERAKPLERESRSSARPPRPSQARE
                     RVASPRRPPLPARQSSRQPASGDTPLPNTGEPQIIVRVRAQFARWAAVSGGLFMINVA
                     TGIEHPWFLIPAAGMGFGLLQGYGKLWQAGYSWRDVLKRPPAPDAVETTLIKGGKLPR
                     QLPPPQASEYGAQLALVLQAHGDRAAVYKLLEKLIPAEREMLPEIGPTVDALYERATD
                     LARTLHAMETNLDTDALGQIESRIQALNREPDDAERARRLALLEKQRRTLTDLEARRS
                     QVSAHLESCVLAVQNVRFDLLRLRSAGVAAVLGDLTNATQQAKALSRDVDNVIAAAGE
                     IREVMR"
     gene            complement(2932..4311)
                     /locus_tag="VFB89_12910"
     CDS             complement(2932..4311)
                     /locus_tag="VFB89_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28242.1"
                     /translation="MLPFANLSADPENEFFADGMTDEVINALAKVPGLRVVSRTSAFA
                     FKGKQLDVREIGAQLNVQAVVEGSVRRSGRRLRLSAQLTNVADGYQLWSESFDRELED
                     VFAVQDELSRGIVSALQIRLLGRQRSALVRPPTDDLEAYTLYLKGRHFWNRRSEPDLW
                     RGLDYFQQALARDLGYAPAHAGVADSYAILGFYSALRPTEAFPQARQAGVRALELDPG
                     LGEAHPALAYVAMYHDWDWAAAEREFQLAIRLNPGYSTTHQWYGNFLAVMGRFDESVR
                     EFSRAIALDPLSALKSAALAWSYYFARRYDLAVAQCLRALELDGELAVAHAWYGLVLE
                     QLKALPEATAAFRAAVRCSGRNVGFLAGLARVLAVAGQAEAGTLLAELIDLRNTSYVS
                     AYDVALIHLALGETGKAMEMLETAYAERAHSMAFMGVDPRLDLLRGEARFERLQARLR
                     FPRSMTAIA"
     gene            complement(4449..5096)
                     /locus_tag="VFB89_12915"
     CDS             complement(4449..5096)
                     /locus_tag="VFB89_12915"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZM28243.1"
                     /translation="MPHLRPQARPPSRAQSPSCQYRRDAWTRAVLREIKLAALLQHPH
                     ILPVFDSGEADGQLWYTMPFVAGESVRSLLDREGRLSLDVTIRIAGQVADALDHAHRH
                     GIVHRDVKPENILLSESHAWLADFGVAQAARSASDRLTEAGMAVGTPAYMSPEQATAE
                     SDLDGRADIYALGCVLYEMLAGKQPYRGGTPAAVLAQQLTGPVPSLRATRADLPS"
     gene            5042..>5228
                     /locus_tag="VFB89_12920"
     CDS             5042..>5228
                     /locus_tag="VFB89_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28244.1"
                     /translation="MKDFERDWAVVLEVVSEVYRRHTGAAELPVDPVMVAEHAAERRV
                     IHGHGVFLANRPENLRRS"
BASE COUNT          955 a         1726 c         1666 g          881 t
ORIGIN      
        1 agctgctcgc gcagaaacat ctcgcgctgc cgctcgccta gctcctcctg gacctgggac
       61 ttgatgtcct cctgagcgtc gaccatgctg atctggcgct gcacgtgcac cagcacccga
      121 cgcagccgct cttcgactga aagggtctcg agcaacgcct gtcgttgctg gggtgtgata
      181 tcgatatatc cggctaccag gtcggcaaac ttgccgggct cgttgacccc ggccagaacc
      241 tgctgcacga cctcttccgg caagcccgac ttctggccca actcggcggc gcgcgcccga
      301 gcctcacggt gcagcgctac gaaggcggtc tcgtcggggc gcaacggaag catttcctcc
      361 gcttcgcgca cgaccgcctg gagatagccg tctttttcgg tatagtggag ggcaatacct
      421 cgctgctcgc catggagcag cagttgcatg cccgaaagcc ctcgctgcat ctgtccgatg
      481 cgcgcgatgg tgccgacggt gtagaggact tcggaggtga cggtttcgac gttctggcgc
      541 tgcgataccg cgaagacctg ccgctccggc gttgtgagcg cagcttcgat tgcgcgcaga
      601 gtaccgggtc gcccggcgcc aatcggcgcg gtgacgcccg gaaacagaac cacctcgcga
      661 agaggaagaa ccggcaatgt gaggcgttca gccatctgaa ttactgctcc tcgtgaatgc
      721 cctcacgggc gcaaatgaca cttcaaacca aacgatcggc cccaagtaag catctggcgt
      781 gccacgggag gcggcatcgt tcgtcttgtg tttcaacagg ttgcggcccc cgctcggttc
      841 cccttggcgc tatcgcccgg ccccaggtcc gctattttgt cggacgaggt ggctggcctg
      901 ccgatttggc ttgataggcg tacagacgag ccgatcggtt gaatccccac acgcggcctc
      961 cgcgtaacac ctccgcgcgc cgtcgtgttg ccatcagcca agccgccagc gcagattctc
     1021 agcgcccaat cacctgaccc tgggttgcaa tgcccctgat cggcgactcg accggcgatt
     1081 cgaccaacca cccgcttccc acccgactct cccgagcgct gggcgacatg tacacgctcg
     1141 aaggcgagat cggtcggggt ggcatgggtg tggtctatcg ggcgcgcgac gagcgactcc
     1201 agcggcgcgt cgccatcaag gtgctgcctc ccgagctcgc cttccagaat gacatccggg
     1261 cccgcttcac cagggaagcc cagaccgctg cccgactctc ccaccctcac atcgtaccta
     1321 tccataccgt cggcgagggc gaagggctgg tctacttcgt catgggctac gtggacggcg
     1381 aatcggttgc cggacgaatc agacgacgcg gcaagttacc ggtcgacgaa gcccgccgca
     1441 ttatggccga gactgccgac gcgctcggcg ccgcccacac tttcggcgtc attcatcggg
     1501 acatcaagcc ggacaacatc ctcctcgagg gaacccgtgg ccgggtcatg gtcactgact
     1561 tcggcattgc caaggcgcta tcacaggcgt ccggcgccac cctgaccggc gccggcgtcg
     1621 cgatcggcac gccgtccttc atgagtccgg agcaagcggc tggcgagcgc gaaatcgacg
     1681 gaagaagcga tatctacagc ctgggcgtgg tcgcgtacca gatgcttacg ggtgatctcc
     1741 cattttacgc gcccacggtc gcgggaatcc tgatgaagca gatcacggag cccgcgccgg
     1801 acgttcgcac caagcggccc gatatacccg aagatctcgc actggccgtg agccggtgcc
     1861 tcgagaaaga tccgcagaac cgttggccca ctgcggacgc attgagacgt gggctggaga
     1921 atcgcagcgt cggcggatat cggcctacgg gaaccggctg gagaaccgga caggccgcgg
     1981 agagagccaa gcccctggag agggaatccc gcagcagcgc ccgtccgccg cgcccgtccc
     2041 aggcgcgcga gcgagtggcg agcccgcgcc gtccaccgct tccagcgcgt cagtcgagcc
     2101 ggcagccggc ttcgggggac acgccgctcc caaacaccgg cgagccccag atcatcgtcc
     2161 gggtccgggc ccagtttgca cgctgggccg ccgtatcggg cgggcttttc atgatcaacg
     2221 tggcgaccgg gatcgagcat ccctggttcc tcattcctgc ggcagggatg ggattcgggc
     2281 tgcttcaagg gtatgggaag ctctggcagg cgggatacag ctggcgcgac gtcctcaagc
     2341 gacctccagc cccggacgcg gtcgagacca ccctcatcaa gggcggcaag ctgcctcggc
     2401 agttgcctcc gcctcaggcc tcggagtacg gggcccagct cgcccttgtc cttcaggccc
     2461 atggcgatcg cgccgcggtc tacaagctgc tcgagaagct gattccggcc gagcgggaaa
     2521 tgctgcccga gatcgggccg acggtcgatg ctttgtacga gcgtgcgacc gatctcgccc
     2581 ggaccctcca cgcgatggag accaacctcg acaccgacgc gctaggtcag atcgagtcgc
     2641 ggattcaagc cctgaatcga gagccggacg atgccgagcg cgcgcgccgg ttggcgctgc
     2701 tcgaaaagca gcggcgtacc ttgaccgacc tcgaggcgcg ccgatcgcag gtgagtgcac
     2761 acctcgaatc gtgcgttctc gcagtgcaga atgtccgctt cgatctgctc aggctgcgct
     2821 cggccggtgt cgcagcggtc ctcggagatc tcaccaacgc cacccagcag gccaaggccc
     2881 tttcccgtga cgtggacaac gtaatcgctg ccgccggcga gatccgcgag gtcatgcgat
     2941 agcagtcatc gagcgaggaa atcggagccg cgcctgaagt cgttcgaatc gcgcctcccc
     3001 acgcagcaaa tccagtcggg ggtccacccc cataaacgcc atcgaatgag cccgctcggc
     3061 gtacgccgtt tccagcattt ccatggcttt ccccgtctca cccaacgcca gatggatcag
     3121 agcgacgtcg tacgctgaca cgtaactggt attgcgcagg tcgatcagct cggcgagcaa
     3181 cgtgccggct tctgcctggc ccgccacggc gagaactcgt gcgaggccag ccaggaatcc
     3241 gacgttgcga ccggaacagc gtacggccgc acgaaatgcg gctgttgctt caggcagcgc
     3301 cttcagctgc tccagcacca gcccgtacca ggcgtgcgca accgcgagct ctccgtccaa
     3361 ctcgagcgcg cgcaggcact gcgcgacggc aaggtcgtag cgccgtgcga agtagtagga
     3421 ccacgcgagt gcagcgcttt tgagcgcaga cagagggtcg agcgcaatgg ctcggctgaa
     3481 ctccctcacc gattcgtcga atcggcccat gaccgcgagg aagttgccgt accactgatg
     3541 ggtcgtggag tagcccgggt tcaacctgat cgcgagctgg aattcgcgct ctgcagcggc
     3601 ccagtcccag tcgtgataca tcgcgacgta ggcgagagcc ggatgtgcct cgcccaggcc
     3661 ggggtcgagc tcgagcgccc ggacaccggc ctggcgagcc tggggaaagg cttctgttgg
     3721 acgcaatgcc gagtagaagc ccaggatggc atacgaatcg gcgacgccgg catgggccgg
     3781 ggcgtagccc aggtcccgcg ccagtgcctg ctggaaatag tcgagacctc gccacagatc
     3841 aggctcgctg cggcgattcc agaaatgacg gcctttgaga tagagggtgt aggcctccag
     3901 atcgtccgtg ggcggtctta ccagagccga gcgttgacgt cccaggagtc ggatctggag
     3961 tgcgctcacg atgccgcgcg agagctcgtc ctgcaccgca aagacatctt ccagctcacg
     4021 gtcgaaactc tccgaccata gttggtagcc atcggcaacg ttagtgagct gcgcgctcag
     4081 gcgcagtcgc cggcccgagc ggcgaacgct gccctcgacc accgcctgga cgttcaactg
     4141 agccccgatc tcgcgaacgt cgagctgctt ccccttgaac gcgaatgcgg acgtgcggga
     4201 gacgacccgg aggccgggca ctttcgccaa tgcgttgatg acctcgtccg tcatgccgtc
     4261 ggcaaagaac tcgttctccg gatcggcgct gagattagcg aacggcagca ccgcgatgga
     4321 cttgtcggct gaagcggcgg gagcgagctc ggtgctccgc gggccaccaa agcggctgca
     4381 agagccgatg ccgaggcgaa ccggtcggcg ggtgacttgg acagagtctt cgtcacgacg
     4441 gaatcgatct aggatggcag atccgcacgg gtcgctcgga gcgaggggac cggaccggtg
     4501 agctgctgtg ccaggaccgc cgccggcgta ccgcctcgat acggctgctt tccggccagc
     4561 atctcgtata gcacacaacc cagcgcgtag atatcggcgc gtccgtccag gtccgactcc
     4621 gcggtggcct gttccgggct catataagcc ggggtgccga ccgccatacc agcctctgtc
     4681 agtcgatcgc tggcgctcct cgccgcctgg gcgaccccga aatcggcgag ccaggcgtgt
     4741 gactccgaca gcagaatgtt ttccggtttg acgtcgcgat gcacgatccc gtggcggtgg
     4801 gcgtggtcca gcgcgtccgc cacctgacct gcaatcctga tagtcacgtc cagcgagagc
     4861 cggccttcgc gatccagcag gcttctgacg ctttcgcccg ccacaaaggg catagtgtac
     4921 caaagttggc cgtccgcctc tcccgaatcg aagaccggca ggatgtgggg atgctgaagc
     4981 agggcggcaa gcttgatctc gcgaagaacc gctcgggtcc aagcatcgcg gcgatattgg
     5041 catgaaggac tttgagcgcg actgggcggt cgtgcttgag gtcgtaagtg aggtataccg
     5101 tcgccatacc ggcgcggccg agctcccggt cgatccggta atggttgcag agcacgctgc
     5161 cgagcggcgg gttatccatg gacacggggt cttcctcgcg aacagaccgg agaatcttcg
     5221 gagaagcg
//
LOCUS       DASRJQ010000566         2049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_170488, whole genome shotgun sequence.
ACCESSION   DASRJQ010000566 DASRJQ010000000
VERSION     DASRJQ010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170488"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..236)
                     /locus_tag="VFB89_12925"
     CDS             complement(15..236)
                     /locus_tag="VFB89_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28245.1"
                     /translation="MVEFVLTPPFLIPVAIAAVLGAAMMKTPEKAPDSYHLTVDIPKA
                     TYDALARQATADTPVRTIASRIVVNGAIR"
     gene            468..1441
                     /locus_tag="VFB89_12930"
                     /pseudo
     CDS             468..1441
                     /locus_tag="VFB89_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015496121.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            1841..>2049
                     /locus_tag="VFB89_12935"
     CDS             1841..>2049
                     /locus_tag="VFB89_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28246.1"
                     /translation="MNVIKVLIDVDQHHDQPLRLESLRKLLQEGQLVRVAEVIDENSD
                     LVGQVGRQTPGQEVGGVVQLPRDLE"
BASE COUNT          362 a          634 c          689 g          364 t
ORIGIN      
        1 agcttccatc ggattcagcg gatggcgcca ttgacgacga tccgcgatgc gatcgtccgg
       61 accggagtat ccgcagtcgc ttgccgcgcc aaggcgtcat aggtcgcttt tggaatgtcg
      121 acggtcaggt gataggagtc cggcgccttt tcaggtgtct tcatcatcgc cgctcccagg
      181 acggcggcga tggcgaccgg gattagaaac ggcggcgtca gtacgaactc taccatcacg
      241 aattctcctt tagcagcgac tggaggggca tcaaggccgc ttgccgatgt caggcctgga
      301 gtccagtatg cttcggatca ccacaccaga tcgtatgatg caggatatcg gcaaggctca
      361 attcgcgtta ggtcggtacg gctacccatg gtagaatcca tcgcgtattg atggagtttg
      421 taatggaaca cggcgtcttt cgggcgtggc ttgcggaggt ggacgacctg accgtcgacc
      481 aacgcctgga gcttgaagag gtgctggccg gacagcctcc gcgcgcggcg gtagcggcgg
      541 tgatcgagac gagcccgggt gacgaacggc gctgcccgca ctgcggtcac ggggcgtcgg
      601 tcggctgcgg tcaggccgat ggactgcggc ggttccgttg caagggatgc ggcaggagct
      661 tcaacgcgct gaccggcacg ccgttggccc ggttgcggaa gaaggagtgc tggctcgact
      721 tcggccggtc gctgagcgag ggcgagacgg tggtcgcctc ggccgagcgg tgtggcgtgg
      781 cggtcagcac cgcgtttcgc tggcgccacc gctttctcac cgccccggtg gctagtccga
      841 tcttgactgg catcgtcgag gccgatgacc cgaagggcag cgcagcaaaa ccttcgtgct
      901 gctcagttat aagggctccc gggcctggga acgcgcggaa cagggccggc ccggagccga
      961 agcgccggat cgcgaggccc gcgggcgcgg cggcaaggcc acgaagcggg gcctgtcgca
     1021 tgagcaggtg ccgatcctgg tggcggccga ccgaagcggc accgtggtca gcgccgtcct
     1081 gccggtggac agcgcggacg ccatcaaggc cgtgctcgaa cccgtcctca gcaaagacgc
     1141 cctgctggtc accgacggcg gcacggccct ggcatgctgt gccaccggga tgaagatttc
     1201 ccacgaggtg ttgaaccaat cggctggcga gcgggtgcgc ggcgacctgc acatccagac
     1261 cgtcaacagc ctgcatgagc ggatcaagac cttcctgcgc gcccggcgcg gcgtggccac
     1321 caaatacctc gacaactacc tgcgatggtt ccatctcgcc ggactccagc acgccccgac
     1381 accccgcgcc tgcctcaatg ccgccattgg gccacaactc aagatggctg taccgaccta
     1441 acgccaattg agccgtggcc aaataggtcc gcgttgcctc ggcccccgcc tccacgctgt
     1501 agcgctgggc tggcagttcc gggatgttca cgatcccgcg ccggttccag tactgcttga
     1561 agaggcaccg gcgcctaaga gccgtggaga gtgtcggcgg ccccggcatg aagcccggac
     1621 ggcggtaggg ctttgttccc agataggcgc cgatctcgtc catcgcgatg cttgtgtcgc
     1681 aagtgaccgc cgggacgccc ttgatctggc tgtcccgcgc caggacgatc aggggcggaa
     1741 ggcccggtcc tagccgcttg tcgcgcaagg tttcgttccg gaacgatgtt ccaaacagca
     1801 caatggcgtc gacctgccgc tggtcagcgt tgagcaaggc atgaacgtga tcaaagtgct
     1861 gattgatgtt gatcagcatc acgatcagcc cctccgcctg gagtctctcc gtaagcttct
     1921 ccaggaaggg cagcttgtgc gggttgccga agtcatcgac gaaaacagcg acctggttgg
     1981 tcaggttggt cgccagactc ctggccagga ggttggggga gtagttcagc ttccgcgcga
     2041 ccttgagca
//
LOCUS       DASRJQ010000567         2606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1951916, whole genome shotgun sequence.
ACCESSION   DASRJQ010000567 DASRJQ010000000
VERSION     DASRJQ010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1951916"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..1197)
                     /gene="speY"
                     /locus_tag="VFB89_12940"
     CDS             complement(25..1197)
                     /gene="speY"
                     /locus_tag="VFB89_12940"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF001980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HZM28247.1"
                     /translation="MTRPSAPYLRGQRINPTAISGRETTADLIDSAFLAYNAGRLREG
                     CALFTHRMLAQDVTVGMSLTGAMTPAGLGMSCLIPLMEAGFVDWVISTGANLYHDAHF
                     GLGLSMHRGTPHADDVELRDKGVVRIYDIFFDYSVLLSTDAFIREVSGQDEFQRPMSS
                     AEYHWLLGKYLRERETALGLDRRSVLAAAYELDVPIYTSSPGDSSIGMNVAEQALAGS
                     RLRFDVSADVNETAAIVLDAKRSGGKSGCLIVGGGSPKNFMLQTEPQIQEVLGIDERG
                     HDYFLQMTDARPDTGGLSGATPAEAVSWGKIDPDQLPGTVVVYMDNSVALPLLTAYAL
                     ARHEPRTLKRLQRRRSAILERLVTEYRDALAQRDRRAEESSLGVHQGAGGAKPAAR"
     gene            complement(1194..1850)
                     /locus_tag="VFB89_12945"
     CDS             complement(1194..1850)
                     /locus_tag="VFB89_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HZM28248.1"
                     /translation="MRILGEFVWGFDNLADVSGGVSIFGSARTRAEDPHYQAAVETAR
                     LLAEAGIPVITGGGPGIMEAANKGAFEANGLSIGCNIELPFEQGTNAYVTRGLMFKFF
                     FVRKMMFVKYAAAFIVFPGGYGTLDELFEALTLIQTGKVKHFPVILFGRRYWGGIIDW
                     LRDTVAAERKVNPEDLDLFRLTDDPAEAVRMVIEAREGDATNGASGSRHTREHERGTV
                     "
     gene            complement(1985..>2606)
                     /locus_tag="VFB89_12950"
     CDS             complement(1985..>2606)
                     /locus_tag="VFB89_12950"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZM28249.1"
                     /translation="ALADIAHRRGALLLIDGYHAVGQLPVDIGALDPDFYCAGGLKWL
                     LGGSGIAFLYVRPELTGTLQTRATGWFGHRDQFRFDPGELVLHDDARRFEAGTPGMGA
                     VYAQLGGLDVLEELGAAEIRRVTLALTEELIERARAAGLRPKVAVDPAVRSGIVMLPS
                     DDPAGDVRRLASANIITDVRPGHVRVSPYFYNLSDDFGALLELLKS"
BASE COUNT          456 a          863 c          823 g          464 t
ORIGIN      
        1 cggcgactgc ctccgcgctc ccgctcaccg ggcggccggc ttggcgccgc ccgcgccttg
       61 atgcacgccg agcgaggact cttccgccct ccggtcgcgc tgcgccagtg catcgcggta
      121 ctccgtcacc agcctttcca ggatagcact gcgcctccgc tgcagccgct tgagtgtccg
      181 gggctcatgg cgggcaagcg catacgcggt gaggagaggc agggccaccg aattgtccat
      241 gtaaacgacc acggttccag gcagctggtc gggatcgatc ttcccccagc tcactgcttc
      301 ggccggcgtc gcgccggaca agccgccggt atccggccga gcgtcggtca tctggaggaa
      361 gtaatcgtgt ccccgctcgt cgatccccag gacctcctgg atctggggct cggtctggag
      421 catgaagttc ttcgggctgc ctccgccgac gatgaggcag ccgctctttc cacccgagcg
      481 tttggcgtcg aggacgatgg cagccgtctc attcacatcc gcgctcacat cgaatcgcag
      541 tcggctgccg gcgagtgcct gttccgccac gttcattccg atggagctgt cgccgggcga
      601 gctggtgtag atgggcacgt ccagctcgta cgccgctgcc agcacggaac ggcgatcgag
      661 tccgagcgct gtttcccgtt cccgcaggta cttgcccaga agccagtgat attcggcgct
      721 gctcatcggg cgctggaact cgtcctggcc agagacttca cggatgaatg cgtcggtcga
      781 gagcaggacc gagtagtcga agaagatgtc gtaaatccga accacgcctt tgtcccggag
      841 ctccacgtcg tcggcatgcg gtgtacctcg atgcatcgag aggcccagtc cgaaatgggc
      901 gtcgtggtag agattcgctc cggttgaaat gacccagtct acgaaccccg cctccatgag
      961 cggaatgagg cagctcattc cgagcccggc gggtgtcatt gccccagtga ggctcatccc
     1021 gacggtcacg tcttgggcca gcatgcgatg cgtaaataga gcgcagcctt cgcgcagccg
     1081 gcccgcgttg taggccagga atgcgctgtc gatcaggtcg gccgtggtct cgcggccgct
     1141 gatggcggtg gggttgatcc gctggccgcg cagatagggc gccgaaggac gggtcacacc
     1201 gtgcctcgct cgtgttcgcg ggtgtggcgg gatcccgacg cgccgttggt agcatcaccc
     1261 tcgcgcgctt cgatcaccat gcgcactgcc tctgccggat cgtccgtcaa ccggaagagg
     1321 tccaggtcct ccggattgac cttccgctcg gcggcgaccg tgtcgcgcag ccagtcgatg
     1381 atgccgcccc agtaccggcg gccgaacaga ataaccggga agtgcttcac cttgcccgtt
     1441 tgaatcagcg tcaacgcctc gaacagctcg tccagagtgc cgtagccacc cggaaaaacg
     1501 atgaacgccg ccgcgtactt cacgaacatc atcttgcgca cgaagaaaaa cttgaacatc
     1561 aggccgcggg tcacataggc gttggtaccc tgctcgaatg gcagctcgat attgcatcct
     1621 atggacaggc cgttggcttc gaaggcgccc ttgttggcgg cctccatgat gcccggtcca
     1681 ccgccggtga tgacagggat gccggcctcc gccagcagac gggctgtttc gacggctgcc
     1741 tgatagtgcg ggtcttccgc acgagtgcgt gccgagccga atatcgaaac cccgccgctc
     1801 acatccgcca ggttgtcgaa accccaaacg aactcgccca gaatccgcaa cgcacgccag
     1861 gaatcggatg ccttgtgcag ggcgatggca tcgcggggtg gagggctctc gagaagcgcc
     1921 tcgtcctcgg tttccttcgt gtctcggagg ggacgggccg ggcccggccg caggggatcg
     1981 tcactcacga cttgagcagc tccagcagcg cgccgaaatc atccgacagg ttatagaagt
     2041 agggagacac ccgaacgtgg cctggccgca cgtccgtgat gatattcgcc gacgccaggc
     2101 gtcgtacgtc gccggcagga tcgtcgctcg gcagcattac gatgcccgat ctcacggccg
     2161 gatcaacggc caccttgggt cgcaatcccg ccgctcgggc ccgctcgatc aattcctccg
     2221 tgagcgccaa ggtgactcgg cgtatctcgg cagcacccag ctcttccagg acgtccaggc
     2281 cgccgagttg ggcatagacg gcacccattc ctggagtccc ggcctcgaac cgccgggcgt
     2341 cgtcgtgcaa caccagctct ccagggtcga agcgaaactg gtccctgtgg ccgaaccatc
     2401 cggtcgcacg ggtctggagt gtcccggtga gctcgggtcg tacgtagaga aaggcaattc
     2461 cggatccacc caggagccac ttgagcccgc ccgcacaata gaaatcgggg tcgagcgcgc
     2521 caatgtccac cggcagctga cctacggcgt ggtagccgtc gatcagcagc agagcccccc
     2581 gtcggtgggc gatgtctgcc agggct
//
LOCUS       DASRJQ010000568         3582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2908097, whole genome shotgun sequence.
ACCESSION   DASRJQ010000568 DASRJQ010000000
VERSION     DASRJQ010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2908097"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..207)
                     /locus_tag="VFB89_12955"
     CDS             complement(49..207)
                     /locus_tag="VFB89_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28250.1"
                     /translation="MPIQRPLNDDSVAARAPTRQITVLAQDPSVRRDSKIVMARIDVP
                     AEDLAAGR"
     gene            complement(333..1646)
                     /locus_tag="VFB89_12960"
     CDS             complement(333..1646)
                     /locus_tag="VFB89_12960"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZM28251.1"
                     /translation="MSGGGYRATIFHAGALRRLNELGLLYQVKEISSVSGGSITTGEL
                     AVFYKKLGGRPHDVISTERWNKEFAEPIHELTGKNIRSRPILEKIFLPWNWFRPDFGV
                     QSMASELDRHFGGVRLIDLGEEPDFIFQATDLSFGVSWEFRPWRVGSYKAGYLQPPPD
                     SWSLGQAVAASAAFPPVFAPMVVPAPSSSSYESYYVEKDQWDKQKPARRQRWKRIPAD
                     GCWKSSDWPFAMQNLRLQDGGVYDNLALEPIWKDRKIVLVSDAGGPFSNQPDQGLFKR
                     GLRWTGIIDNQSRALRKRMLIGLDDHDMTISYWATDHPSDRSSRAKGYSAELAEDYIA
                     QIRTDLDVFSEAERAILENHGYLAADEAIFKTVCHRKRPDERKPDLRGCAPSKTLPTT
                     ELPYEFAHLANSWPALNPPHLSWLPGAKTECQIKKALFSNDSCKD"
     gene            complement(1792..2175)
                     /locus_tag="VFB89_12965"
     CDS             complement(1792..2175)
                     /locus_tag="VFB89_12965"
                     /inference="COORDINATES: protein motif:HMM:NF023288.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramidase domain-containing protein"
                     /protein_id="HZM28252.1"
                     /translation="MSQRHTRARSIGQMDITPFLGSGRPLSDQGMDLVCDSLSVTESE
                     IWAVLSVETRAFGFLPDRRPLILFERHVFHRLTQGRHSAVHPEISNATPGGYLGGAAE
                     YKRLARPSRSTARRRSRARHGASAR"
     gene            complement(2419..2652)
                     /locus_tag="VFB89_12970"
     CDS             complement(2419..2652)
                     /locus_tag="VFB89_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28253.1"
                     /translation="MAYRVGKAKAITATARKLAILVYRVLKDGLVYRDLGAAAYDAQY
                     GQRVIRRLRQRAAEVGFALVDRGTGELIEGAVS"
     gene            2731..2937
                     /locus_tag="VFB89_12975"
     CDS             2731..2937
                     /locus_tag="VFB89_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28254.1"
                     /translation="MLRALPPRPEGPPAVFAVEPMPFALRARAREAGGSLFPDGFAVV
                     TAGAVVTGPRPVAWSREAGGFKRV"
     gene            complement(3377..>3582)
                     /locus_tag="VFB89_12980"
     CDS             complement(3377..>3582)
                     /locus_tag="VFB89_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28255.1"
                     /translation="FASMHESGNTARRLDLVDDPIPVPYRLHRYWGASLTPREKASKR
                     PALVLDPFLSDHLPSGRATEASV"
BASE COUNT          605 a         1114 c         1164 g          699 t
ORIGIN      
        1 ggaacacacg ccgcgtcgcg tcgaaatcaa ccacctggac gcgatagcct atcggcccgc
       61 cgccagatcc tccgccggca catcgatcct cgccatcacg atcttcgagt cgcgccgcac
      121 gctgggatcc tgcgcgagga cggtaatctg ccgcgtcggt gcacgcgccg cgacggagtc
      181 atcgtttagt ggcctttgga taggcattgg caccctcggc agtaagagag ttaggaaatg
      241 tgagagtggg agttggaaac acgcaggcaa ttgcattgct tgttatccac gtgcgctcct
      301 cggcccgcac gaagccgtgc tgctgttcga cgttagtcct tgcatgaatc gttactgaac
      361 aaggccttct tgatctgaca ctctgttttc gcgcccggga gccagcttag gtgcggcggg
      421 ttgagggctg gccaagaatt ggcgagatga gcaaactcgt acggaagttc cgtcgtcggg
      481 agtgttttcg aaggtgcgca acctctcaga tcgggtttcc tctcgtcagg gcgttttcgg
      541 tgacatacgg tcttgaagat cgcctcatct gctgccaggt agccgtggtt ctcgagaatg
      601 gcgcgttcgg cttcgctgaa gacgtcgagg tcggtgcgga tctgggcaat gtagtcctcc
      661 gccagctcgg ccgagtaccc tttcgccctt gatgagcggt cgcttggatg atcggtcgcc
      721 cagtagctta tggtcatgtc gtggtcgtcg aggccgatga gcatgcgctt tctcaatgct
      781 cgagactggt tgtcgatgat acccgtccag cgcaggccgc gtttgaacag gccttggtcg
      841 ggctgattcg agaacggtcc gccggcatcc gacaccagta cgatctttcg gtccttccaa
      901 atgggctcga gcgcgaggtt gtcgtatacg ccgccgtcct ggagtcggag gttctgcatg
      961 gcgaagggcc agtcactaga tttccagcag ccgtcagcag gaatgcgttt ccagcgctgc
     1021 cgcctggcgg gtttctgttt gtcccactgg tccttctcga cgtaatagct ctcgtaggaa
     1081 ctacttgacg gggctggcac taccatgggc gcgaacaccg gcgggaaggc cgccgacgca
     1141 gcgaccgcct gccccaagga ccacgagtcc ggcgggggct ggaggtagcc cgctttgtag
     1201 ctgcccactc gccagggtcg gaactcccag ctcacgccga aggagagatc ggtggcctgg
     1261 aagatgaagt caggctcctc gcctaggtcg atgaggcgca ctccgccgaa atgccgatcg
     1321 agctcgctcg ccatactctg cacgccgaag tctggtctga accaattcca gggcaggaag
     1381 atcttctcga ggatcggcct ggagcggatg ttctttcccg tgagctcgtg aatcggctcg
     1441 gcgaattctt tgttccacct ctcggtggag atcacatcgt gaggcctgcc gccgagcttc
     1501 ttgtagaaca ccgctagctc cccggtcgtg atgctgccgc ctgagaccga cgagatctcc
     1561 ttgacctggt aaagcaggcc gagctcgttc aggcgtcgca gcgcgccggc gtggaagatg
     1621 gtggcgcggt agccgccgcc ggacattgcg agtccgatcc cctgggcctc tgttgcccgg
     1681 tcggggcagt cctttggctc ggcccgatac gagcccggtg gccgggcacc gctgcagccg
     1741 cgaccatcgc ctccacgtcc ttgaagccca cagcggagaa gttgaagccc atcacctggc
     1801 cgatgcccca tgacgcgctc tggagcgccg ccttgcggtc gagcgcgatg gcctggcgag
     1861 ccgcttgtac tccgcggcac cgccgaggta gccgcccggc gtcgcgttgc tgatttccgg
     1921 gtgcaccgcg ctatgcctgc cctgcgttag ccggtggaag acgtggcgct cgaacaggat
     1981 gaggggtctc ctgtcaggca ggaacccgaa agcgcgcgtt tcgacgctga gcaccgccca
     2041 gatctcagac tccgtcacgg acaggctatc gcacaccagg tccataccct gatcgctcag
     2101 cgggcgcccg cttcccagaa agggcgtgat gtccatctgc ccgatggagc gagctcgcgt
     2161 gtgccgctgg ctcatgccga tgggaacata ctgccacgtc cctgggcggg tcaatcgcga
     2221 cctgcgccga ttgaacctcc tcgaggttga ccctcttatg ctttcgttcc ctcctttcca
     2281 agatcgccga atggggcgca acatcggaac aggcgaagtc atcggatcga ccgcggcatc
     2341 aaaccgcctc ctgggggagc gagagcaacg atggaggggc ggtgcctgct gagaatgggc
     2401 ctttatgtcg cctttctcct aagaaactgc accctcgatc agctcgccgg ttccacgatc
     2461 gacgagggcg aagccgacct cggccgcgcg ttgacgcaag cggcgaatga cgcgctggcc
     2521 gtactgcgcg tcgtacgcgg cggctcccag atcgcggtag accagaccat ccttgagcac
     2581 gcgatagacg aggatggcga gcttgcgggc cgtcgcggtg atcgctttgg ccttgcccac
     2641 ccggtaggcc aagcgttcgc tcccggctcc gatcctcggc tcgcctttgg cggctcgggc
     2701 ttcgcgatcg aacgcgagac agttgcggcc gtgctgcggg ccctgccgcc gaggcctgag
     2761 ggtccgccgg cggtcttcgc cgtcgaacca atgccgttcg ccctgcgcgc ccgcgcccgc
     2821 gaggcgggcg gcagtctctt tcccgacggg ttcgcggtcg tcaccgccgg ggcggtggtt
     2881 acagggcccc gcccggttgc ctggtcccgc gaggcgggcg gcttcaagag ggtgtgagcc
     2941 ggcggcgatt gtgccttggc ggcacccgac tctgaaacat tcttgcggca gagggtcaag
     3001 cccaaggctg ctgggtaatc gtgaactgcg atgcactcta cgttgacctg aatggtgcgc
     3061 gattcgggtg atacggagat agtggcccac ctctctgcgg tcgaccgcac gaaggggttt
     3121 ccagacggcg ccttccaggc tccggcgtcg tatctctgac ctttcaaacc actgttgggg
     3181 cggtgtccgc tatggccacg ccgcgaatta ggaaggtcag gtcgaggcct actaccgcac
     3241 aaatcccgtt gatgggatga aagcgctgcg cctccgcaca cggcgaggta ccgtgtgcgt
     3301 cggtaaacac tcagcggtgt cagccgcgag aggagatgcc gccgatgcag tacgtcgcgt
     3361 tcgatgccca caagcattac acgctcgcct cagtcgctcg gccggacggc aggtggtccg
     3421 agaggaacgg atcgagcacg agcgcggggc gcttcgaggc tttctcacgc ggtgtgagcg
     3481 aggctcccca gtagcggtgg agacgatagg ggactggtat tggatcgtcg acgagatcga
     3541 ggcggcgggc tgtattcccc gactcgtgca tgctcgcaaa gc
//
LOCUS       DASRJQ010000569         2120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_566535, whole genome shotgun sequence.
ACCESSION   DASRJQ010000569 DASRJQ010000000
VERSION     DASRJQ010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566535"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VFB89_12985"
     CDS             <1..331
                     /locus_tag="VFB89_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28256.1"
                     /translation="HDALKRRCLYHWIDHPQLTREIEIIRTRLPDVSTELAAQVASAV
                     QRIRTNEDILKPPGVAESLDWARALQRLGHDHLDVAAAAETLGAVLKYREDAERVRRA
                     LEAILAR"
     gene            338..1450
                     /locus_tag="VFB89_12990"
     CDS             338..1450
                     /locus_tag="VFB89_12990"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZM28257.1"
                     /translation="MTSSKVPAPEELLVGFATALREAGVAVTPDRTSSFVQAVALTGF
                     GDRASTYWSGRATLCSSLDDTELYDRAFTAWFTISPDVPRAENRISPTRQPQAVFDDD
                     SSSGNQDGDEILAARATTTEVLRHRDVATLSDQERTRLATLFDSLDVVPPLRRALRQR
                     PHHRGSVDLRRTLRAQLRSAGELTRLRHRRPGLRPRRVVLLIDVSGSMQPYADSLLRL
                     AHRIVRAMPHHVEVFTIGTRLTRISAALRLRQGDEALAAAGRLVPDWSGGTRLGEVIG
                     AFVNRWGRRGVARSAIMVIASDGWERGDAQLLSEEMRQLRLLAYAVIWMNPHHGKAGY
                     APIQTGIQASLPHLDRMVAGHSLATFAELLEVLADA"
     gene            1462..>2120
                     /locus_tag="VFB89_12995"
     CDS             1462..>2120
                     /locus_tag="VFB89_12995"
                     /inference="COORDINATES: protein motif:HMM:NF014662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HZM28258.1"
                     /translation="MRDVLNDLLAWWRAGETVGMGTVVGTWRSAPRQPGAAMLVGPDD
                     SAVGSVSGGCVESAVYALAKDVAADGHPMLQRYGVSDNDAFAVGLTCGGILDVFVEKV
                     SRETFPELADIAADIDAGRPVAVATLVDHPDAKRLGMRMIIRPAEDSPIFGSLGSARA
                     DAAVADDVRGLLAAGKTELLTYGPEGQRRGEGMRVFAASYAPRPRMLVFGAIDFAAAV
                     AR"
BASE COUNT          356 a          664 c          720 g          380 t
ORIGIN      
        1 gcatgatgcc ctcaagcggc gttgccttta ccactggatc gaccatccgc agctcacccg
       61 tgagatcgag atcatccgga ctcggttgcc cgatgtaagt accgaactcg cggcacaggt
      121 ggcctccgcg gtgcagcgca tccgaaccaa cgaggacatc ctgaaaccac caggagtcgc
      181 cgaaagtttg gactgggcgc gtgccctgca acggctcggg catgatcacc tggacgttgc
      241 cgcggcggct gagacgctcg gcgcggtgct caagtaccgg gaggacgccg agcgggtgcg
      301 gcgcgcattg gaagcaattc ttgctcggta gccaaagatg accagctcaa aggttccggc
      361 gccagaggag ctcctggtcg gtttcgccac tgcgctccgc gaggcgggag tggccgtcac
      421 tccagaccgg acgagttcat ttgttcaggc agttgccttg accggcttcg gtgatcgagc
      481 ctcgacctac tggtctggcc gcgcaacgtt gtgctcatca ttggacgaca ccgagctcta
      541 cgacagggcg ttcacagcct ggttcacgat ttcaccggac gtgcctcggg ccgaaaacag
      601 aatttcacca acaagacagc cgcaggcagt cttcgatgac gactcgagct ccggtaatca
      661 agacggggat gagatcctcg ccgcgcgcgc aaccacaacg gaggtcttgc gccatcgcga
      721 tgtggcaacc ctcagcgacc aggagcgaac ccggttggcg accctcttcg acagcctcga
      781 cgtggtacca ccgttgcgga gggcacttcg acaacggccc catcatcgcg gttcggtcga
      841 tctgcggcgt acgctgcgcg cgcagctgcg ctcagccggc gagctgacta ggctccgcca
      901 ccgacggcct ggccttcgcc cccggcgggt tgtgctgctg atcgatgtct caggctctat
      961 gcagccgtac gcggactctc tgttgcgcct ggcgcatcgg attgtccggg caatgcctca
     1021 tcatgtcgag gtctttacca ttggcacccg gctcaccagg atcagtgcgg cactccgcct
     1081 gcgacagggc gacgaggcac tcgcggcagc gggacggctg gtgcccgact ggtcaggcgg
     1141 cacccggctg ggcgaggtga tcggcgcgtt cgtaaaccgc tggggacgcc gcggcgtcgc
     1201 gcgaagcgcg atcatggtca ttgcgagtga cggctgggag cggggcgacg cgcagctgct
     1261 cagtgaggag atgcggcagc tgcggctgct cgcgtacgcg gtgatctgga tgaacccgca
     1321 tcatggaaaa gcggggtacg ctccgatcca gaccggtatc caggccagtc tgccccacct
     1381 tgatcgaatg gtcgcggggc actcgctggc gacattcgct gaacttttgg aggtgctcgc
     1441 cgatgcttga ggggtgcacc gatgcgtgat gtgctcaacg acctcctggc atggtggcgg
     1501 gcgggcgaga cggtcggcat gggcaccgtc gtcggcacct ggcgttcggc acctcggcag
     1561 cctggtgccg ccatgctggt tgggccggat gactcggcgg tgggctcagt ctccggaggc
     1621 tgtgtggaat ccgcggtcta tgcactcgcg aaagacgtgg ctgctgacgg ccatccgatg
     1681 ctgcagcggt atggcgtcag tgacaacgat gcgttcgcgg tcggcctgac ctgcggcggg
     1741 attctcgacg tcttcgtcga gaaagtatcc cgagagacgt tccctgagct cgccgacatt
     1801 gcggccgaca ttgacgccgg ccgtccggta gccgtcgcaa cgctggtaga ccatccagat
     1861 gccaagcggc tgggcatgag gatgatcatc cggccggcgg aggactcccc catcttcggt
     1921 tcgttaggca gcgcgcgggc tgacgccgcc gttgccgatg atgtgcgcgg gctgctggca
     1981 gccggcaaga ccgagctgct cacgtacggc ccggaggggc agcggcgcgg cgagggaatg
     2041 cgcgttttcg cggccagcta cgcgcctcgc ccacgaatgc tggtcttcgg ggcaatcgac
     2101 tttgcggctg ctgtggcacg
//
LOCUS       DASRJQ010000570         3191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3147444, whole genome shotgun sequence.
ACCESSION   DASRJQ010000570 DASRJQ010000000
VERSION     DASRJQ010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3147444"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..1014
                     /locus_tag="VFB89_13000"
     CDS             178..1014
                     /locus_tag="VFB89_13000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZM28259.1"
                     /translation="MQGRTSLVTGANRGIGFAIAHGLAAQGARVILVVRDENAGNAAA
                     EEIRRVTENDAVDVLVADLSSQASIREVAEQVLTRYRRLHVLVNNAGVITRRRSLTVD
                     GIETQFAVNHLAYFLLTSLLLPRLRASAPARIVVMASDAHWTHELDFEDLQLSRGWRP
                     VRAYSQSKLANVLFAYELARRLSGTGVTVNCAHPGGVATKLLASAFPLPAITTPLIYR
                     FAASPARGAETPLWLATAPELEEVTGKYFVRWKAVPSSAATYDLFAARQLWEESERLT
                     GL"
     gene            complement(1011..1973)
                     /locus_tag="VFB89_13005"
     CDS             complement(1011..1973)
                     /locus_tag="VFB89_13005"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HZM28260.1"
                     /translation="MGSKPVLRPPRLTAGSRIGLVSPSGPVLDGDDLARAGELCRELG
                     HAPLVGANAHRRHGYLAGTDAERIADLNAALRDESLDAVWCVRGGYGLTRILDRIDFA
                     PLERRPKALIGFSDITALLLAAHQVIRIVGFHAPTARQPMPPFSRRHFTQVLATAQPA
                     GRLDQREPPAGVLAPRSPRIVKIHGGAAEGTLIGGNLSLLQCLVGTRWFPEIDGALLF
                     LEDVGEDLYRVDRALSHLRLAGVLERIAGIIVGQFTDMKLGTGDGALSFGEVLEHYVG
                     PLGIPAAYGFPIGHVDEQWTMPIGVRARLDADSGQVDILEAAVT"
     gene            2036..2599
                     /locus_tag="VFB89_13010"
     CDS             2036..2599
                     /locus_tag="VFB89_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28261.1"
                     /translation="MRASQPALLAAGRVISDMRDVMVTQWADWVGDRITAASTIPRTA
                     VEREFRLIVDTMTAMVGPLRREARVTWTRVCEHYGRVAAARGLAAGEVVEELQYLREL
                     LIRNLAHAFFSMRPRQALAIVLRLNRVLDKGIAVAVVGYTDALVATLFAQNGVPGPSS
                     EHDASELDRQLLALETELGDLAQARKR"
     gene            2612..>3191
                     /locus_tag="VFB89_13015"
     CDS             2612..>3191
                     /locus_tag="VFB89_13015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HZM28262.1"
                     /translation="MTALAALWPALPWLGPLLGLLRFARRGPDLLQFPLSSGRLISVI
                     VPARNEAATIETVMCSVLTSGYEPLELIVVDDRSTDDTAAIAERVAAGDSRARLIRGK
                     ELPSGWFGKQWACIQGYQAARGELLVFTDADTMHDRELLGRAAGALAATEADLLTVAT
                     RQRCETFWERVVMPQIWMLLGFRYHPRTVSRSR"
BASE COUNT          551 a         1038 c         1036 g          566 t
ORIGIN      
        1 catcaccgaa gccgagcgcc tggtcaaggc cgcgaatcct gcatcgtccc gcttccttcg
       61 actcgaaggc gctgagcaca ccttcggagc cgctcatccc tggaagggct caaccgcggc
      121 actcgatctg gccatgaaag aaacggtcgg gtggctgtcc aaacacctgc tctgacgatg
      181 cagggacgga cgtctctagt caccggcgcg aaccggggga ttgggttcgc catcgcccac
      241 gggctggcag cccagggggc ccgcgtaatc ctggtcgttc gagatgagaa cgccggaaac
      301 gcggctgcgg aggagattcg tcgcgtcacc gagaacgacg cggtggacgt tctggttgcc
      361 gacctctcct cacaggcctc gattcgagag gtggctgagc aggtattgac ccggtaccgg
      421 cggctgcacg tgctggtgaa caatgccggc gtcatcacgc gtcggcgctc gctgactgtc
      481 gatggcatcg aaactcaatt cgccgtcaat catctcgcct atttcctgct cacttctttg
      541 ctcctgcccc gcctccgggc gtcagcgcca gcgcggatcg tggtcatggc gtcggatgct
      601 cactggacgc acgagctgga cttcgaggac ctgcagcttt cccgcggatg gcgaccggtt
      661 cgggcgtatt cgcagagcaa gctggccaac gtgctttttg cctatgagct ggcgcgtcgc
      721 ctgagcggca caggggtgac cgtcaactgc gcccatcccg gcggcgtcgc caccaagttg
      781 ttggcgagtg cgttcccgct gccggcgatt acgacgccgc tgatctatcg ctttgcggcc
      841 agccctgcgc ggggcgccga gacgccgctt tggctggcca cggcgccaga gttggaagag
      901 gtaacgggga agtacttcgt gcggtggaag gcggtgccgt cgagtgcggc aacttacgat
      961 ctgttcgccg cccgccagct ctgggaagaa agcgagcgcc tgaccggtct ctaggtaact
     1021 gctgcctcga ggatgtcgac ctgaccgctg tcggcatcga gtcgtgcgcg gacaccgatc
     1081 ggcatggtcc actgctcgtc cacgtggccg atgggaaatc cgtacgcagc cggaatgccg
     1141 agcggtccaa catagtgctc gagaacttcg ccaaacgaaa gggcgccatc tccggtaccc
     1201 aatttcatat cggtgaactg gcccacgatg atccccgcga ttcgctccag cacccccgca
     1261 aggcggaggt gcgagagggc ccggtccacc cgatagagat cctcccccac gtcctccaga
     1321 aacagaaggg cgccgtcgat ctcagggaac caacgcgtcc ccaccaggca ttgcaaaagg
     1381 gaaaggttgc ccccgattag cgtcccttcg gcagctccac cgtggatttt cacaattcgc
     1441 ggagagcgtg gagcgagcac gccggccgga ggctccctct ggtcaagccg gcctgccggt
     1501 tgagccgtgg cgaggacctg agtgaagtgg cggcgcgaga acggcggcat gggttggcgg
     1561 gcggttggcg cgtgaaagcc gacgattcgt atgacttggt gtgccgccag gagcagcgca
     1621 gtgatatcgg agaatccgat gagcgccttg gggcggcgct ccagcggcgc aaaatcgatt
     1681 cggtcgagga tacgggtgag gccgtagccg ccccgcacgc accagacggc atccagcgat
     1741 tcgtcccgca gggcggcatt gaggtcagca atgcgctcgg catcggtacc ggccaaatag
     1801 ccatggcgcc gatgggcgtt ggcgccgacc agcggcgcgt gaccaagctc acggcagagc
     1861 tcgccggcgc gcgctaagtc atcgccgtcg agtaccggtc ccgaagggga gaccagccca
     1921 atacgacttc cagcggtcag acgaggaggg cgcagcactg gttttgaccc catcctcatc
     1981 ccctccttag attgaccagc caagctggat ccaacctcat cacaggagta cggcagtgcg
     2041 tgcttcgcag cccgcgttgc tcgccgccgg ccgagtgatc tccgatatgc gggacgtgat
     2101 ggtcacccag tgggccgatt gggtgggtga ccggatcacg gccgcttcca cgataccgcg
     2161 tacggccgtc gagcgggagt ttcgactcat cgtcgacacc atgaccgcaa tggtcgggcc
     2221 cctccgccgc gaggcgaggg tgacctggac ccgtgtctgc gagcactatg gacgcgtggc
     2281 agcggcgcgc gggctcgccg ccggcgaggt ggtggaggag ctgcagtatc ttcgggaact
     2341 gctcatccgc aacctggcac acgctttctt ttccatgcga ccgcgtcagg cgcttgctat
     2401 cgttctccgg ctcaatcgcg tgctcgacaa ggggatcgcg gtcgcggtag tgggctacac
     2461 cgacgcgctg gtggccacgc tgtttgccca gaatggcgtt cccggcccca gcagcgaaca
     2521 cgatgccagc gagctcgacc gccaattgct cgcgctcgag accgaactgg gcgacctcgc
     2581 gcaagcgcgg aagcgctagc gcccctttcc cttgacagcc ctcgccgcac tctggcccgc
     2641 ccttccctgg ctggggccgc tcctcggtct tttgcggttt gcccggcgcg gccccgacct
     2701 cctgcaattt cccctctcct ccggccgcct gatcagtgtc atcgtcccgg cgcgcaacga
     2761 agccgcaacg atcgagaccg tcatgtgctc cgttctcacc agtggctatg agccgctcga
     2821 gctgattgta gtcgacgacc gatctaccga tgataccgcc gcaattgcgg agcgcgtggc
     2881 ggccggcgat tcacgagcgc gcctgattcg cggcaaggag cttccctcgg gctggttcgg
     2941 gaagcagtgg gcctgcattc agggttatca ggcggctcgg ggtgagctac tggtatttac
     3001 cgacgccgac acgatgcatg accgagagct gctaggccga gccgcagggg cgcttgccgc
     3061 aaccgaagcc gacctgctca ccgtggctac gcggcagcgc tgcgaaacct tctgggaacg
     3121 agtggtaatg cctcagatct ggatgctgct cgggtttcgc taccatccgc ggacggtgag
     3181 tcgctcccgg c
//
LOCUS       DASRJQ010000571         2219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3927558, whole genome shotgun sequence.
ACCESSION   DASRJQ010000571 DASRJQ010000000
VERSION     DASRJQ010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3927558"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1731
                     /locus_tag="VFB89_13020"
     CDS             <1..1731
                     /locus_tag="VFB89_13020"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZM28263.1"
                     /translation="LEVFTHPQAGLQKPLLDADTQRALAGELNAPDPGARAVAVSLLG
                     RLGDGVQTDLIVGALQDADWRVRLAALQALIDQGHPEAAGATITMLEDSAPQVRSLAA
                     RSISRLGTDYAESLAAILEDPDPGVRAVAATGVGGIRAQKTLDRMLESDSPADQEAAL
                     RALASHPDLTELDLVRFAGHPDRRVRAAAAAALAPLAGSGPAIRSLLDDGSVIVRRAS
                     AAALAQRADGPALFMDVLANGSVRATEAALQSLADTGQTGEGLTEWASQEVARAAFLR
                     RHRLALATSDSSATRTVLATLLASRQERLLRWALLATTTPHTADMMTVVGRGLRSADQ
                     ETRAQAVEALESVGVHAVTRSLIPLLEETGHGALPDSRTSLRELSEDHDEWIRALAVR
                     CITEELIDDLGHLRGLADADQSGLVQSAIARWEVIAVQDTQTLDTVDRVVALQRVPMF
                     AGIDPEDLERIATLFTERRYDAGELIFAWGAEGDEMLIIVEGEVTVSRPQEGAEVPIR
                     TVRSGDYVGELSLLRGQPRAADVTAGPEGVRGLILRGAQLHAILEERPEAAMAMLATL
                     AERLGTDWNR"
     gene            1896..>2219
                     /locus_tag="VFB89_13025"
     CDS             1896..>2219
                     /locus_tag="VFB89_13025"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HZM28264.1"
                     /translation="MSEQYVLGLQEVDESQVAVVGGKGAHLGALSRIKGIHVPAGFCV
                     TTDAFRRIMAEAPSIDDQLDQLSRLNPDDRDAIRMLSAEIRSRVEAIPIPDDLAAAIT
                     RALAHP"
BASE COUNT          381 a          728 c          753 g          357 t
ORIGIN      
        1 ctcgaggtct tcactcatcc gcaagcgggg ctgcagaagc cgctgctcga cgccgacacc
       61 cagcgcgcac tcgctggtga attgaacgct ccggaccccg gggcacgggc ggtagccgtc
      121 tcgctcctcg gtcgcctggg ggatggagtc cagacagatc tcatcgtcgg ggcgttgcag
      181 gatgccgact ggagggtcag gctcgccgcc ctccaggcgc tgatcgacca gggacaccct
      241 gaggcggccg gcgccacgat caccatgctc gaagactcgg cgccacaggt ccgttctctg
      301 gcagcccggt ccatctcacg gcttggcacc gactacgccg agtctctcgc cgccatcctg
      361 gaggatcccg atcccggtgt cagagctgtc gcagccaccg gtgtgggtgg gatcagggct
      421 cagaagacgc tggatcggat gctcgagtcc gacagcccag cagatcaaga ggcagcgctc
      481 cgagcgttgg cgagccaccc cgatctcacc gagctagatc tggtccgatt tgccgggcat
      541 ccggaccgac gagtccgcgc ggctgctgcc gcggcgctgg cgccgctagc cggctcgggc
      601 ccggcgatac gtagcctgct cgacgacggc tcggtcatcg tgcgccgggc atcggccgcc
      661 gctctcgccc agcgcgccga tgggccggct ctcttcatgg acgtgctggc aaacggatcg
      721 gttcgcgcca cagaggccgc cctgcagtcg ctggccgaca ccgggcaaac cggtgaaggg
      781 ctcacggaat gggcgtccca ggaagtcgcc cgcgccgcct tcctgcgccg gcaccgtcta
      841 gcgctggcca ccagcgacag ctctgccacg cgtacagtcc ttgcgactct gctggcctcg
      901 cgccaggagc gcctcttacg ttgggcgctg ctggctacga ccacaccgca cacggcggac
      961 atgatgacgg tggtcggaag gggattgcgc tcggcggatc aagagaccag ggcacaagcg
     1021 gtggaggcgc tggaatctgt cggggtgcat gcggtcacca ggtccctcat tccacttctg
     1081 gaggagacgg gccacggcgc gctgccggat tctcggacgt cgctccgcga gctgtcggag
     1141 gaccacgatg aatggatccg tgccctggcg gtacgctgca tcaccgagga gctcatcgac
     1201 gacctcgggc acctccgggg cctggcggat gccgatcagt cgggcctggt ccaatcggcg
     1261 atcgcacgat gggaggtcat cgcggtgcag gacacccaga cactcgacac ggtggaccgg
     1321 gtggtcgcat tgcagcgagt gccgatgttc gccgggatcg acccggaaga tctcgagcga
     1381 atcgccactc tcttcaccga acgtcgctat gacgccgggg aactcatctt cgcctggggc
     1441 gcagagggcg acgagatgtt gatcattgtc gagggcgagg tgacggtgag ccgcccgcaa
     1501 gagggtgccg aagtacccat ccgaaccgtc cggtcgggcg attacgtcgg ggagctctcg
     1561 ctgttacgcg gtcaaccgcg cgcggccgat gtcaccgccg gtcccgaggg cgttcgcggg
     1621 ctcattctga ggggagccca gctacacgcc atcctcgagg agcggccaga agccgccatg
     1681 gccatgctcg cgactctggc ggaacggctc ggcacggatt ggaaccggtg atctgttatg
     1741 tcgtcacgca gcggaccaca aaaggtaagg caaggagatc gcactcctcg ccacttttaa
     1801 cgtacaggag actggggggc ttgccgcaag acccgggtcg tgctgcagaa tcatcggccg
     1861 gacgaacaac ggacgaagac ctcggaggta tcgcggtgag cgagcagtac gtgttgggtc
     1921 ttcaagaggt tgacgagtcg caggttgcgg tcgttggcgg caaaggcgcg cacctgggcg
     1981 cgctgtcgcg gatcaaaggc atccatgtgc cggctggctt ttgcgtgacg acggacgcct
     2041 tccggcgcat tatggcggaa gcgccctcga tcgacgacca gctcgatcag ctgtcgcgcc
     2101 tgaacccgga cgaccgtgac gcgatccgca tgctcagcgc ggagatccga agtcgcgtcg
     2161 aagcgattcc catccctgac gatttggcgg cggcgatcac ccgcgcactc gcccaccca
//
LOCUS       DASRJQ010000572         4975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1780435, whole genome shotgun sequence.
ACCESSION   DASRJQ010000572 DASRJQ010000000
VERSION     DASRJQ010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4975
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1780435"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VFB89_13030"
     CDS             complement(<1..443)
                     /locus_tag="VFB89_13030"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZM28265.1"
                     /translation="MARARGGLRLFIVASLSLAAVESGIAQHSGGKSTIATFRGDAAH
                     AGVYAAPTPAAYAGLAWRVQTGGTVVSSPAVGESTIYIGSNDRHLYAIDRVSGRVRWT
                     FDANSAVGSSPAVALGIVAFTTADGHIFAVDANTGRLRWKLATGST"
     gene            complement(528..1832)
                     /locus_tag="VFB89_13035"
     CDS             complement(528..1832)
                     /locus_tag="VFB89_13035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZM28266.1"
                     /translation="MDVVIIGGGAAGLAAARVLAKAGVSVELLEAQERLGGRILTVED
                     SAGRPLELGAEFIHGSPGTTLALAEEAGLPLVPVATPHWELRNGALELADRVFDGVAQ
                     LMRQAQGHGEETSVALFLKRFDENHPLHDAAEWVRRLVEGFDAADPTQASLAAIVDEW
                     TGGAGVQRSQSRIEGGYGRLIAYLASFAKANGVETLLGTAVTGVSWKPNEVAVEAVAG
                     GQPYRVVARCAILTLPLSILQSKSKEVPAVSFTPALTSKADALDHLIMGDVLKVILRF
                     REPFWETISDGRFRGAAFFHARDQEFPTFWTEPPDQGPVLTAWTGGPRAERLSSADDT
                     TIIESAVNGLGAVFAGKIDVASHLVDARLHNWQRDPFSRGAYSYVAVGGRKARSGLAA
                     ALENTLFFAGEATDESGEAGTVAGALASGERAAAQVLATFKA"
     gene            complement(1835..2998)
                     /locus_tag="VFB89_13040"
     CDS             complement(1835..2998)
                     /locus_tag="VFB89_13040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZM28267.1"
                     /translation="MTSADVVVLGAGVMGASAAWHLARRGVRNVLLLDAAATAGAGST
                     GRATGGFRAQYATAINVRLSLLSREKLKAFADDTGVDPGYAQHGYLWLARTDRELALL
                     AEARRVQHEAGLSEARGVTPDEIRELNPALAAEGIVGGAFCQTDGYLLPLNILEGYLA
                     GARSMGAEVRWGVRVTGLEVSQGGRVTAVRAGGERIGCGAVINALGPWAATIMETTGF
                     VLPVSPLRRQIAATVPTTLLPATTPMTIFAGDGFHFRVRDGRVLLLWPTPGVSGRPYD
                     ASVDPGWVSQVTAMAHARVPCLRAIPIDPDACWAGLYEISPDKHAILGPTPGAPNLYL
                     INGSSGHGVMHAPALGQLLAEIVCDGRASTLDAAPLAPERFADGRMNPVSELL"
     gene            complement(2995..3408)
                     /locus_tag="VFB89_13045"
     CDS             complement(2995..3408)
                     /locus_tag="VFB89_13045"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZM28268.1"
                     /translation="MTAFHFAFFVQDLASTRRFYRDVLGCREGRSTDSWVDFDFFGHQ
                     ISAHVTGPVVPTVDAGVVDGVRVPMPHFGALLAWDEFARVAEGIRQAEIPFIIEPRIR
                     YPGQPGEQMTMFLRDPSGNALEFKSFKHPEHVFTA"
     gene            complement(3405..4634)
                     /locus_tag="VFB89_13050"
     CDS             complement(3405..4634)
                     /locus_tag="VFB89_13050"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HZM28269.1"
                     /translation="MVAPAKVAAIPAISLAEVEAREPARRRLDSVDLLRGVVIVVMAL
                     DHVRDYFTRSAHLFDPADLTRTTAPLFLTRWITHYCAPTFVFLAGASAFLYGSRPGRT
                     QKELARFLLSRGLWLVFIELTVVRLGWYFNFDYHFFHVQVIWAIGWSMVALAALVFLP
                     IRVVGAIGIAMIATHNLLDGISVSPLLAAPGVFAHASLWDWLWSVLHVRNLPVAYPLV
                     PWIGVMAAGYAFGPVLHHTPDQRRRELLQLGTLLVVGFVVLRAINVYGDAQRWSVQST
                     SLFTFFSFLNTTKYPPSLLYLLMTLGPAILSLVFFERLRGPVARFFILYGRVPFLFYV
                     AHIYLIHALVLLVAGFRGDDLHRYLVFWRMMPETWGYSLGVAYAVWIFVVLALYPACR
                     WFAGVKARRRDAWLSYL"
BASE COUNT         1011 a         1641 c         1533 g          790 t
ORIGIN      
        1 gtggagccgg tcgcaagctt ccatcgcaaa cgaccggtgt ttgcatcaac ggcaaagatg
       61 tgcccgtcag ctgtcgtgaa ggccacgatg cccagagcca cggctggaga ggagcccacc
      121 gcgctgttcg catcgaacgt ccagcggacc cgacccgaga cccggtcgat cgcatacagg
      181 tgccgatcgt tgctgccgat gtagatagtg gactcaccga cggcgggtga cgagacgacc
      241 gtgcctccgg tctgaactcg ccacgccagc cctgcgtagg cagccggcgt cggcgccgcg
      301 tacacgccgg catgcgcagc atcgccccgg aaggtcgcga tcgtactctt tccacccgag
      361 tgctgcgcga taccgctctc cacggccgcg agtgagagag aagcgactat gaacagccga
      421 agcccgccgc gtgcgcgcgc catgcagatg ctcccggatt gattggcgac tttactgcga
      481 tcgactcgtg accgtacgga tgcggatgga gaatggtgtc aggtctgtca cgccttgaac
      541 gtcgcgagca cctgagcggc ggcgcgctca cccgatgcaa gcgctccggc aacagtgccg
      601 gcttcgccgg actcgtcggt tgcttcgccg gcgaagaaga gcgtgttctc gagcgcggcc
      661 gcgagcccgc tcctggcctt acgaccgccc acggcgacat agctgtaggc accacgcgaa
      721 aacgggtcgc gctgccagtt gtgtagccgg gcgtcgacca gatggctcgc cacatcaatc
      781 ttgccggcga agaccgctcc aagtccgttc actgcgcttt caatgatggt cgtgtcgtct
      841 gcactcgaca gtcgctcagc acgggggcct ccggtccacg ccgtcaggac tgggccctga
      901 tccggtggct cggtccagaa cgtcggaaac tcctgatccc tggcgtggaa gaaggccgca
      961 ccgcgaaaac ggccatccga gatcgtttcc cagaatggct cgcgaaaccg aagaatcacc
     1021 ttgagcacgt cgcccatgat aaggtgatca agcgcatcgg cctttgacgt aagcgcagga
     1081 gtgaacgaaa cagctggtac ttccttcgac tttgactgaa ggatgcttaa cgggagggtg
     1141 aggatggcac agcgcgcgac cacgcgataa ggctgtccgc cggccactgc ctccaccgcg
     1201 acctcattcg gcttccagga cacaccggtg accgccgtcc caagcagggt ttcgacgcca
     1261 ttcgccttcg caaacgaagc gaggtacgcg atcagccgcc cgtagccacc ttcgatccgg
     1321 ctctggcttc gttgcacgcc cgcgccgcca gtccactcat ccacaatggc ggcaagactc
     1381 gcctgcgtgg ggtccgccgc atcgaatcct tcgacgagcc gtcgcaccca ctccgcggcg
     1441 tcatggagtg gatgattctc gtcgaatcgc ttgaggaaca atgcaacgga cgtttcctcg
     1501 ccatggccct gcgcctgacg catgagctgt gcgacgccat cgaacacacg atcggccagc
     1561 tcgagcgctc cgttccggag ctcccagtga ggcgtcgcga ccggcacgag cggcagtccc
     1621 gcttcctctg caagcgccaa ggtagtcccg ggacttccgt gtatgaactc cgcacccagc
     1681 tcgagcgggc ggccggccga gtcttccaca gtcaggatcc ggccgccaag ccgctcctgc
     1741 gcctcgagca gctcgaccga gacccctgcc ttggccaata cccgcgccgc tgcgagcccc
     1801 gcagctcccc cgccaatgat cacgacatcc atctctatag gagctcggaa acggggttca
     1861 ttcttccgtc ggcaaatcgt tcgggtgcga gcggcgcagc atcgagagtg gatgcgcggc
     1921 catcgcacac aatttcggcc aggagctggc cgagcgctgg cgcatgcatc accccgtggc
     1981 cggacgaccc gttgatcagg tacaggttgg gagcgcctgg cgtggggccg aggatcgcat
     2041 gcttgtcggg agagatctcg tagagtcctg cccagcaggc atccggatcg atggggatcg
     2101 cccgcaggca cggcaccctg gcatgcgcca tcgcggtgac ctgcgagacc caaccggggt
     2161 cgaccgaggc atcgtatggg cgtccggata cgcctggggt gggccagagg agtagcacgc
     2221 ggccatcccg gacgcggaag tgaaatccgt caccggcgaa aatcgtcatc ggtgtcgtgg
     2281 cagggagcag ggtggtcggc accgtcgcgg ctatctgacg tcgcaacggt gagaccggta
     2341 gcacgaagcc cgtcgtttcc atgatcgtcg ccgcccaggg gccaagtgcg ttgatgactg
     2401 caccacaccc aatccgttca ccacctgccc ggaccgcagt cacccggccg ccctggctca
     2461 cctcgagccc ggtgacccga acgccccaac gaacttcggc gcccatgctt cgagcgccgg
     2521 cgagatagcc ctccaggatg ttgagtggga gaaggtatcc gtcggtctgg caaaacgcgc
     2581 cgcccacgat gccttcggct gcaagggccg ggttgagctc ccggatctcg tccggggtca
     2641 ccccgcgagc ctcgctcaac ccggcttcgt gctgtacccg tcgtgcctcc gccagcaacg
     2701 cgagctcgcg gtccgtacgg gcaagccaga ggtagccgtg ttgagcatag ccgggatcca
     2761 cgccggtgtc gtcggcgaac gccttcagtt tttcccggga aagaagggag aggcgcacgt
     2821 tgatggcggt tgcgtactgg gcgcgaaacc cgcctgtagc gcgaccagtg ctccccgccc
     2881 ccgccgtggc cgcggcgtcg agcagcagca cgtttcgcac cccgcgccga gccagatgcc
     2941 aggcggcgct ggctcccatt acgcctgcgc ccagcaccac cacatcagcc gaagtcacgc
     3001 ggtgaagacg tgctccggat gcttgaagct cttgaactcc agagcgttac cgctcggatc
     3061 gcggagaaac atcgtcatct gctcgcccgg ctggccggga tatcggatgc gtggctcgat
     3121 gatgaacgga atttccgcct gccgaattcc ctcggccact cgggcaaatt catcccatgc
     3181 cagcagtgca ccgaagtgag gcatgggtac ccgaacgcca tcgactacgc cggcgtcgac
     3241 tgtcggcacc acagggcccg tgacgtgggc cgagatctgg tgcccgaaga agtcgaagtc
     3301 cacccagcta tcggtgctcc gtccttcccg gcagcccagc acatcacgat agaatcgtcg
     3361 ggtagaggcg agatcctgca cgaagaaagc gaagtgaaac gcggtcacaa gtagctgagc
     3421 caggcatcgc gccggcgcgc cttcactccc gcaaaccagc ggcatgcggg gtagagagcc
     3481 aggaccacga atatccaaac cgcgtaggca acaccgaggc tgtagcccca cgtttccggc
     3541 atcatgcgcc agaacacgag gtacctatgc aggtcatcac cccggaagcc tgccaccagc
     3601 agaaccaggg cgtggatcag gtagatatgg gcgacataga aaaggaaagg gacccggccg
     3661 tacaggatga agaagcgcgc caccgggccg cgcagccgtt cgaagaacac cagcgacaga
     3721 attgccggtc ccagcgtcat gagaagatac aggagcgacg gagggtattt cgtcgtgttg
     3781 aggaacgaga agaacgtaaa cagcgaggtg gactgaaccg accaccgctg ggcatctcca
     3841 tacacattga tggcgcgcag cacgacgaag cccacaacca gcagcgtacc cagttgcagt
     3901 agctcgcgcc gacgttgatc gggtgtgtgg tgcaggaccg gcccgaaagc ataacccgcc
     3961 gccatcaccc cgatccacgg aaccagtgga taggcgaccg gcaagttgcg gacgtgcagg
     4021 acgctccaca gccaatccca caggctggcg tgggcgaaca cgccgggagc cgccagcaac
     4081 ggactcacgc ttatgccgtc gagcaggttg tgagttgcga tcatcgcgat gccgattgcg
     4141 cccaccactc gaatgggcag aaacaccagc gcagccagtg ccaccatgga ccagccgatt
     4201 gcccagatca cctgcacgtg gaaaaagtga tagtcgaagt tgaagtacca gcccaaccgc
     4261 accaccgtca actcgatgaa caccagccac aggcctcgac tcagaagaaa tcgcgccagt
     4321 tctttttggg tgcgtcccgg gcgggaaccg tagaggaacg cgctggcacc ggcgaggaac
     4381 acaaacgtcg gcgcacagta atgcgtgatc cagcgagtga gaaagagggg ggccgtcgtc
     4441 cgagtcaggt cggccggatc gaacaggtga gccgagcgcg tgaagtaatc ccgcacgtga
     4501 tcgagcgcca tgacaacgat gaccacacct cgcaggaggt cgaccgaatc gagccggcgg
     4561 cgcgcgggct cgcgggcctc gacctctgcg agagatattg ctggtatcgc ggcaacctta
     4621 gcgggggcga ccatcgactg agcttccttg aacgggtgag gggctgggaa tcgctcaata
     4681 tgggtcgggc cgccacctcc gtcgagggtc aaatggatta gcttcgggcc ggcacagcaa
     4741 agtcctacat atcgttactc attggttcta cacgccacta ctcatgagtc ctacacgtca
     4801 caactctctc cgcagcgccg gcgaggaaca caaacgtcgc cccagccccc accaaccgtc
     4861 actcgtgcga cggccctctt cccgcttcca cccgatacag cagatccgcc ccggtggcct
     4921 cgccggtttc agcctgcaag gtccatttgc cggagagtat gtatcgaagc ctcaa
//
LOCUS       DASRJQ010000573         2082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3189117, whole genome shotgun sequence.
ACCESSION   DASRJQ010000573 DASRJQ010000000
VERSION     DASRJQ010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3189117"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1325
                     /locus_tag="VFB89_13055"
     CDS             <1..1325
                     /locus_tag="VFB89_13055"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZM28270.1"
                     /translation="LEYDGHEVLTAGSGPEALTVAERENPDLVFLDIKMPGLDGLEVL
                     SRLRGTNETLPVVIISAHGTAATALEAGRLGAFRFIEKPLSKDYVLDAIREGLELGSL
                     RRENRQMRSALETRHEMVGSSSTLKQILDQVRRAAPTNATVLILGESGVGKELIARAI
                     HRSSLRAKERFVQVNCAAIPEELIESELFGHERGAFTGATEKQTGKFEQADRGTIFLD
                     EVGDMSPKTQAKVLRVLQEGEVERLGSARTIKVDVRVIAATNKDLEEEIAEGRFREDL
                     YFRLSVIPISVPPLRERLDDIPSLVQHFVGQFSRENNRRQARFAPGALDTLRQARWRG
                     NIRELRNVVERLLIMSDREVIEPEDVRSVVRPDARPTGVAAQVTGATAAKPATLREFK
                     EWSERAFLVEKLREFGWNISKTAEVIDTPRSNLYKKLEQYGIKQEGET"
     gene            1479..2003
                     /gene="rnpB"
                     /locus_tag="VFB89_13060"
     ncRNA           1479..2003
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFB89_13060"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT          402 a          648 c          730 g          302 t
ORIGIN      
        1 ttctagaata cgacgggcat gaggtgctca cggcggggtc cggccccgaa gcgctgaccg
       61 tcgccgagcg cgagaacccg gacctggtct tcctcgacat caagatgccg ggcctcgacg
      121 ggctggaagt gctgagccgg ctccggggca cgaacgaaac gctgccagtg gtcatcatct
      181 cggcgcacgg cacggcggcc acggcgctcg aggccgggcg gctcggcgcg tttcgcttca
      241 tcgagaagcc gctctccaaa gactacgtgc tggacgcgat tcgcgaggga ctcgagcttg
      301 gatcgctccg ccgggagaac cgccagatgc ggtcggcgct ggagaccagg cacgagatgg
      361 tcggcagcag ctcgacgctg aagcagatcc tggatcaggt gcgccgcgcc gcgccgacga
      421 atgcgacggt gctgattctc ggcgagagcg gcgtgggcaa ggagctcatc gcgcgcgcga
      481 ttcatcgatc gagcctgcgt gcgaaagagc ggttcgtgca ggtcaactgc gccgcgattc
      541 cggaagagct gattgagtcc gagctcttcg gccacgagcg cggcgcgttc accggcgcca
      601 ccgagaaaca gacggggaag ttcgaacagg ccgaccgcgg gacgatcttc ctcgacgagg
      661 tcggcgacat gagcccgaag acgcaggcga aggtgctgcg cgtgcttcag gagggcgagg
      721 tcgagcggct cgggtcggcg cggaccatca aggtggacgt ccgtgtcatc gccgccacca
      781 acaaggatct cgaggaggaa atcgcggagg gccggttccg ggaggacttg tacttccggt
      841 tgagcgtcat cccgatttcc gtgccccccc tgcgggagcg gctcgacgac atcccgtcgc
      901 tggtccagca cttcgtcggc cagttcagcc gcgagaacaa ccggcggcag gcgcggttcg
      961 cgcccggcgc gctcgacacg ctgcgccagg cgcggtggcg cggcaacatc cgcgagctgc
     1021 gcaatgtggt cgagcggctg ttgatcatga gcgaccgcga agtcatcgag cctgaagatg
     1081 tccgctcggt cgtgcggccc gacgctcgtc ccaccggcgt cgccgcccag gtgaccggcg
     1141 ccaccgccgc caagccggcc acgttgagag agttcaagga gtggtccgag cgcgccttcc
     1201 tcgtcgagaa gctgcgcgag ttcgggtgga acatctcaaa aacggccgag gtcatcgaca
     1261 ccccgcgcag caatctctac aagaagctcg agcagtacgg catcaagcaa gagggtgaaa
     1321 cctagtcagg gcagagggca cagggcagag ggcagaggtg aggggagaaa tcgcaaggcg
     1381 taggtccggc cctctgcctt ttgttcctct tccctcccct ctgccctgtg ccctatgccc
     1441 tctgccctga caagagcctg tacaatgaac tgttcgccgc agcgggccag atgatcgccg
     1501 tctcgccgaa gcctcgccga agcgcccgca agggagcgga ggcggggcgg aggcggaggg
     1561 aggaaagtcc gaactccgca gggcagtgcg ccgggtaacg cccggtcggg gcaactcgaa
     1621 ggacagtggc acagaaaaca taccgccctg agcgaggcct tcggcgcgcg tgaaagcgcg
     1681 cgctcaggcc gagtcgaagg gtaagggtga aaaggtgcgg taagagcgca ccgcgtctgc
     1741 cgtaaggcag atggcaggca aaaccccgca cggagcaagg ccaaataggg aggcgaatcc
     1801 catcggcgca cgcgagtgtg ctggtgacag aggacggccc gtccgaagcc tccgggtagg
     1861 tcgctcgagc ccgccagcaa tgccgggcct agaggaatga ttgtcgcttc gccgcagctc
     1921 gttaccgtcg tcgcggctgc aaggccgcgg aggcgaatga gcgagggcga ggaacagaat
     1981 tcggcttacg tcccgctgcg gcgaacactt cgatttcagg ccaaaactga accgcagtac
     2041 aattcgtcgg tccgctccgg cactcgggtt gccgcggagg ct
//
LOCUS       DASRJQ010000574         7250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2519905, whole genome shotgun sequence.
ACCESSION   DASRJQ010000574 DASRJQ010000000
VERSION     DASRJQ010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7250
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2519905"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /gene="xseB"
                     /locus_tag="VFB89_13065"
     CDS             <1..184
                     /gene="xseB"
                     /locus_tag="VFB89_13065"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HZM28271.1"
                     /translation="EEIVRRLEADDLDLDGALALFEQGVACLRSARERLSAAEVRVRT
                     VLEDANGTLRLGKLDG"
     gene            177..1088
                     /locus_tag="VFB89_13070"
     CDS             177..1088
                     /locus_tag="VFB89_13070"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HZM28272.1"
                     /translation="MGEVVRASAGELLTEARDATDALLERWARRLESSVAAPEGAALA
                     YALRSPGKRVRAALVVAAFRSAGGESAGIVGVAGAVETVHAYSLVHDDLPCMDDDDLR
                     RGRPTTHRQFGVPAATRAGYLLVPVAAMVLAEAARDLELDATGLGNMAAELFQAGGIE
                     GMVGGQWLDLLAEGRLLELPELMAVHRGKTGALIRAACTLGGLAAAAPPSTIAALAGY
                     GEEIGLAFQIADDVLDATATSVQLGKTAGRDATLSKSTYVRVLGIDGARSEAAKLVRR
                     AVAHLEMAGVPTGSLAALADYIVTRSS"
     gene            1214..3112
                     /gene="dxs"
                     /locus_tag="VFB89_13075"
     CDS             1214..3112
                     /gene="dxs"
                     /locus_tag="VFB89_13075"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HZM28273.1"
                     /translation="MSLLATISSPADLKRLRRDQLPLLAREIRERMIECCSVTGGHIG
                     ASLGVVELAVALLYEFDSPRDKIVWDVGHQAYPWKLLTGRNDAFPSLRQSGGISGFLK
                     RTESEHDQFGAGHAGTAVSAALGMATARDLKGESHKVIAVLGDGALTCGLSYEGLNNA
                     GHSDRDLIVILNDNGMSISPNVGGISKMLGSIVANPRTNALREQVKHLTFALGGVFGE
                     SVVDFAKNLEESAKNLFSQGMLFEELGLRYFGPIDGHDVQKLCDTLAFVRGLTGPRLV
                     HVITEKGKGFAFAEANREKWHGLAAYDPETGEPRKKANGPPAWTQVFGDAITSLAAAH
                     PELVAVTAAMPSGTGTNLFQKKWPDRFFDVGIAEGHAVTFAGGLSTQGIRPIVAIYST
                     FLQRAYDNVIHDVAVQHLPVIFALDRAGLVGEDGQTHMGLYDIAYMLAVPGMTVTAPK
                     DGDELIGLLKTALAHQGGPFSTRYPRDKAPAEPRPVADVPAVPYATWEQLRRGRDVGI
                     LAVGTMVMPALGAAELLAADGMDCAVINCRFLKPMDLGLLESLAQRHRVLVTIEEGTV
                     VNGFGAYLAETLQTTHPEVRVVALGVPDRLIEQAPRAEQLQTFGLTPEGIARRIAALL
                     HEESLEAR"
     gene            3109..3978
                     /locus_tag="VFB89_13080"
     CDS             3109..3978
                     /locus_tag="VFB89_13080"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HZM28274.1"
                     /translation="MKVGVVGNPRYADLAAVLERMAREAPPRGITLFTEPRLAPFWPC
                     PIPNLDTVRLDALLTFGGDGTLLRGARLLTDLHTCILGVNLGRVGFLTTATLDTLGQA
                     LDALVSGDYIVERRQALTAEIATGLGEVRHIQTALNDVAIHKGGVARVIRVNVLIEGE
                     NVGPYSADGIVVATPTGSTAYSLSAGGPIVVPGVEAMVVTAICAHTLAVRPLVVPASY
                     TIVIQPMEGWAEDLLVSFDGQTGTTLVAGETVTVRRSTSPVSLVRLARDGFFTRMREK
                     LQWGDLSERERPR"
     gene            3975..5648
                     /gene="recN"
                     /locus_tag="VFB89_13085"
     CDS             3975..5648
                     /gene="recN"
                     /locus_tag="VFB89_13085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HZM28275.1"
                     /translation="MIAELRVRDLATIADVTLPLGAGLNVLTGETGAGKSMLVDALAL
                     LLGDRADSSSVRPGATKAVVEGAFENLEPPVRREIESHGLDLDEGRLIVRREISIEGR
                     SRAWVNGSPTTAGVLARLGAVLVDLHGQHETQSLLRADAQRDILDAFGHAEGERAAMA
                     EAHAALAALRAQEADLAARRDEVRRRADYLLHVVEEIDRAKLRPGEDAALDLEARRLG
                     QAGALTEQARRIVEAVDGDEGSALRALAVADRALSALEKNDPSAGEWRELLDGAFANL
                     GELSRLAGDYAESIQEDPVRLAEVERRRDVISRLSQKHGAGIEAVLETRRRAGEELDL
                     LDTADTDLRALAARRTAAEAALKAAADTLSASRNQAAEQLGRAVSRLLPNLGMSGGKL
                     SVELIPAKEPSSYGQESVAFTVRLNVGLEAKPLAKVASGGELSRLMLGLKVVLAKHDA
                     IPTLVFDEVDQGIGGEVGAQVGDALAAVAARRQVLVITHLPQIAARADRHLVVSKAPR
                     AGIATSELTLIHGEDRIVELARMLGDAEGSEARRHAQALLGRTKAERSN"
     gene            5664..6926
                     /locus_tag="VFB89_13090"
     CDS             5664..6926
                     /locus_tag="VFB89_13090"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HZM28276.1"
                     /translation="MATATLAEAPSIVPSQSTRPRLDSVDFLRGLVMVIMALDHVRDF
                     FNIDARFFDPTDLTRTTPMLFFTRWITHFCAPVFVFLAGTGAYLWASRGRTKTELSRF
                     LVSRGLWLIVVELTLLTVGWTYNFTWSFIGLQVIWVIGWSMILLAGLAWLPLRVIAGI
                     GIVLIVGHNLLDGIHPHPLLAAIPATSGALNPFGQGPIAGVISPEATPWDWLWSFLHV
                     FNPPIAYPLIPWAGVMAVGYAFGPLMKRPPDERRRFCLWLGLSLTAAFVLIRWLNVYG
                     DPSPWKPQADPVYTVLSFLNTSKYPPSLLYLLMTLGPALVLLGLMDRASGPVVRFFVV
                     YGRVPFLYYVVHLYLIHGLTLVAAAITGQAAPGDLRVFFLFYPKTFGFGLGTVYAIWI
                     GVVLALYPLCWWFARLKARRKDVWLSYV"
     gene            complement(6969..>7250)
                     /locus_tag="VFB89_13095"
     CDS             complement(6969..>7250)
                     /locus_tag="VFB89_13095"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HZM28277.1"
                     /translation="TEAAFFAASPGQAITYEIGKLQILAFLTDARQHLGDRFSLRSFH
                     DFLWKNGNVPIALQRWEMLGLDDEVRILDKAGERAKGEKGEKEKGRKVP"
BASE COUNT         1189 a         2254 c         2444 g         1363 t
ORIGIN      
        1 cgaagagatc gttcgccggc tcgaggccga cgacctcgat ctcgacggcg cgctcgcgct
       61 gttcgagcaa ggggtcgcct gtctccgctc ggcgcgggag cgcctgtcag cggcggaagt
      121 gcgggtgcgg acggtgctcg aggacgcaaa cggcacgctc cgcctgggca agctggatgg
      181 gtgaggtggt tcgggcgtcc gcgggcgagc tgctcactga agcccgagat gccactgatg
      241 ccctcctcga gcgctgggcc cgtcggctcg aatccagcgt cgcggcgccg gagggcgctg
      301 ctctggccta cgcgctccga agtcccggca aacgagtccg cgcagctctg gtcgttgccg
      361 ccttccgctc ggcgggtgga gaaagtgccg gcatagtcgg cgttgccggc gccgtggaaa
      421 ccgtgcacgc ctactcgctg gtgcacgacg accttccttg tatggatgac gacgacctgc
      481 ggcggggccg gccgaccact catcgccagt tcggtgtgcc ggccgccacc cgcgcgggat
      541 acttgctagt cccggtcgcg gcgatggtgc tcgcggaagc agcgcgggat ctcgagctgg
      601 acgcgaccgg gctgggcaac atggccgctg agctgtttca ggcaggcggt atcgaaggca
      661 tggtgggcgg ccagtggctc gatctcctcg cagaggggcg ccttctggag ctccctgagc
      721 tgatggcagt ccatcgcggg aagacgggcg cgctgatccg tgcggcgtgc acgttgggtg
      781 gtctcgctgc tgctgcacca ccgagcacaa tcgccgcgct cgccggatac ggcgaagaga
      841 tcggcctcgc cttccagatc gcagatgacg tgctcgatgc caccgccacc agcgtccagt
      901 tggggaagac agccggacgc gacgcaactc tgtccaagtc gacatatgtc agggtgctgg
      961 ggatcgacgg cgcgcgctca gaggcagcga agctcgtgcg gcgggccgtg gcgcacctgg
     1021 agatggctgg agtgccgacg gggtcgttgg cggcgttggc ggactatatt gtgacgagaa
     1081 gtagttgagt gagagctatc agctatcagc tatcagtctc ggcggaagct gagctgatag
     1141 ctgatagctg atagctgata actgatagct gatagctgat agctgatagc tgatagctct
     1201 accccgaggc cccatgtccc tgctggctac catttcctct cccgccgatc tcaagcggct
     1261 gcgtcgggac cagctgcccc tgctcgcccg cgagatccgg gagcgtatga tcgaatgctg
     1321 ctcggtgacg ggtggccaca tcggcgcaag ccttggcgtc gtcgagctgg cggtggcgct
     1381 gctctacgag ttcgattctc cccgcgacaa gatcgtctgg gacgtcgggc atcaggccta
     1441 tccctggaag ctgctcaccg ggcggaatga tgcctttccg agcctgcgcc agtccggcgg
     1501 tatctcgggc ttcctcaagc gaaccgaaag cgagcacgat cagttcgggg cggggcacgc
     1561 cggtaccgcg gtttcggcgg cgcttggtat ggccaccgct cgcgacctga agggcgagag
     1621 ccacaaggtc atcgcggtgc tgggagatgg agctctgacc tgcgggctgt cttatgaggg
     1681 tctgaacaac gcggggcatt cggatcgtga tctgatcgtc atcctgaacg acaacggcat
     1741 gtcgatttcg cccaatgtcg gtggaatctc caagatgctc ggcagcatcg tcgccaaccc
     1801 gcgaaccaac gcactgcgcg agcaagtaaa gcatctcacg tttgcgctgg gcggcgtgtt
     1861 cggcgagagc gtggtggact ttgccaagaa cctggaagag agtgccaaaa acctcttctc
     1921 gcagggtatg ctgttcgagg agctggggct gcgttacttc gggccgatcg acggccatga
     1981 tgtgcagaag ctctgcgaca cgctcgcctt cgtccgcggg ctcaccggac ctcgcctcgt
     2041 tcatgtgatc accgagaagg ggaagggctt cgctttcgcg gaggcgaacc gggagaaatg
     2101 gcacgggcta gcggcctacg accccgagac cggcgagcct cgcaagaagg cgaatggtcc
     2161 acccgcgtgg actcaggtgt tcggcgacgc catcacgtcg ctcgccgccg cgcatcccga
     2221 gctggttgcg gttacggcgg ccatgccttc gggcacgggc accaatctct ttcaaaaaaa
     2281 gtggcccgac cgcttcttcg atgtcgggat cgcggaaggc cacgcggtca ccttcgccgg
     2341 gggcctctca acgcagggaa tccggccgat cgtcgcgatc tacagcactt tccttcagcg
     2401 agcctacgac aacgtcatcc atgacgtcgc tgtgcagcac ctgcccgtga tctttgctct
     2461 cgatcgagcg ggcctggtcg gtgaagacgg acagacccac atgggtctgt atgacatcgc
     2521 atacatgctg gcggtgccgg ggatgacggt gaccgcgccc aaggatggcg acgagctgat
     2581 cgggctgctc aagacggcac tggcacacca gggcggtccg ttctccaccc ggtatccgcg
     2641 ggacaaggct cccgcggagc cgcggccggt ggccgacgtc cccgcggttc cctacgccac
     2701 gtgggagcag ctccgacgcg ggcgagacgt gggtattctc gccgttggca cgatggtgat
     2761 gcctgcgctg ggagccgccg agctgctggc tgccgacggc atggactgcg cggtaatcaa
     2821 ctgtcggttc ctcaagccga tggacctggg gctcctcgag tcgcttgctc agcgacatcg
     2881 cgtgctggtg accatcgaag agggtacggt ggtcaacggg tttggcgcct acctcgccga
     2941 gacgctgcag acgacgcacc ctgaagttcg agtggtcgca ctgggcgttc ccgatcgcct
     3001 gatcgagcag gcgcctcgtg ccgaacagct gcagacgttt gggctcacgc cggaggggat
     3061 cgctcgtcgg atcgcggccc tgcttcacga ggagagcctc gaggcgcgat gaaggtcggg
     3121 gtcgtcggca atccgcggta tgcagatctc gcagccgtcc tcgagcgcat ggctcgcgag
     3181 gcgccgcccc gtggcattac gctgttcacc gagcccaggc ttgctccatt ctggccttgt
     3241 ccgattccca acctcgacac ggtccggctc gacgcattgc ttaccttcgg cggagacggt
     3301 acgctgctcc gcggcgctcg tctgctcacc gacctgcaca cctgcattct gggcgtcaac
     3361 ctggggcggg tcggcttcct gacgacagcg actctggaca cgctgggtca ggcactcgat
     3421 gcgctggttt cgggtgatta catcgtggag cggcgccagg cgctcaccgc cgagatcgcc
     3481 accggtctcg gcgaggtccg tcacatccag acagcgctga acgacgtggc gatccacaag
     3541 ggtggcgttg cccgggtgat ccgggtgaat gtcctgatcg aaggcgagaa cgtcgggccg
     3601 tacagtgcgg acgggattgt cgtcgcgacg cccactgggt ctaccgcgta cagcctgagc
     3661 gccggcggcc cgattgtcgt gcccggcgta gaggcgatgg tcgtcaccgc gatctgtgcg
     3721 catacgctcg ctgtgagacc gctggtcgtg cccgcatcct atacgatcgt gattcagccg
     3781 atggaaggct gggccgaaga tctcctggtc tcgttcgacg gccagaccgg aacgacactg
     3841 gtcgcgggcg agaccgtaac cgtccgtcgc tcgacttctc ctgtatcgct ggttcggctg
     3901 gcacgcgacg gattcttcac ccggatgcga gagaagctcc agtggggtga tctgtcggag
     3961 cgcgagcgcc cgcggtgatc gccgagcttc gggttcgcga tctcgccacc atcgccgatg
     4021 tgacgctccc gctcggagcc ggcctcaacg ttctcaccgg cgaaaccgga gcaggcaagt
     4081 cgatgttggt agacgccctc gccttgctcc tgggcgaccg ggcggacagc tcgagcgtgc
     4141 gccccggagc gaccaaggcg gtggtcgagg gcgcattcga gaatctggag cctccggttc
     4201 ggcgcgagat cgagtcacac ggcctcgacc tcgatgaagg gcggctgatc gtccgacggg
     4261 agatctcgat cgaaggacgc tcccgtgcct gggtcaacgg cagtcccacc accgcaggcg
     4321 tgcttgcgcg gctcggtgca gtgctggtcg atctgcatgg ccagcacgag acccagtcac
     4381 tccttcgggc cgacgctcaa cgtgacatcc tggatgcctt tggtcacgcg gagggcgagc
     4441 gtgcggccat ggcggaggcc cacgctgcgc ttgcggcatt gcgagcgcag gaggccgacc
     4501 tcgccgctcg ccgtgatgaa gtgaggcgtc gggcagacta tctgctgcac gtggtcgagg
     4561 agatcgaccg cgccaagctc cgtcccggtg aggatgccgc cctcgacctc gaggcccgcc
     4621 gcctgggcca ggcgggagcg ctcaccgaac aggcacgtcg cattgtcgag gcggtggatg
     4681 gcgacgaagg ttccgctctc cgggcgctgg cagtggctga ccgcgccctg agcgcgctcg
     4741 agaagaacga tccgtccgcg ggcgagtggc gcgagctgct ggacggcgcg ttcgccaatc
     4801 tcggtgagct gtcccggctg gctggagatt acgccgaatc gattcaggaa gatcctgtcc
     4861 gcctggccga ggtcgagcgc cggcgcgacg tcatctcccg gctcagtcag aagcacggtg
     4921 ccggcatcga ggccgtcctg gagactcggc gacgggccgg ggaagagctc gacctgctgg
     4981 acacggcgga cactgatctg cgtgcgctcg ctgcccggcg cacggccgcg gaagcggcgc
     5041 tcaaggccgc ggccgataca ctctccgcca gtcgaaacca agcggccgag cagcttggcc
     5101 gggcggtgag ccggctgctt cctaacctag gcatgtcagg gggaaagctc agtgtcgagc
     5161 tgatccctgc gaaagagcct tccagctacg gacaggaatc ggttgcattc accgtgcgac
     5221 tcaacgttgg gctcgaggca aagccattgg caaaggtcgc ctcgggcggc gagctatccc
     5281 ggctgatgct cggtctcaag gtggtcctgg caaagcacga cgccattccg accctggttt
     5341 tcgacgaagt ggatcagggc atcggcggcg aggtaggcgc acaggtgggc gacgcgcttg
     5401 cggccgtggc tgctcgacgt caggtcctgg tgatcaccca cctaccgcag atcgccgctc
     5461 gggccgatcg gcacctggtc gtgtcgaagg caccccgggc cggcatagca accagtgagt
     5521 tgacactcat tcatggagag gaccgtatcg tagagttggc gcggatgctg ggcgacgccg
     5581 agggcagcga agctcgccgc cacgcacaag cgctcctcgg tcgaaccaag gcagaaaggt
     5641 cgaactagct agccggagag atcatggcca ccgctacgct ggcggaagcg ccctctatcg
     5701 ttcccagcca gagcactcgc ccccggctgg attcggtgga tttcctgagg ggtctggtca
     5761 tggtgatcat ggccttggac cacgtcaggg atttcttcaa catcgacgcc cgcttcttcg
     5821 acccgaccga cctcacccgt accactccga tgctcttctt cactcgatgg atcacccatt
     5881 tctgtgcgcc ggttttcgtc ttccttgctg gaacgggagc ctacctttgg gcgtcgcgcg
     5941 gacgcaccaa gaccgaactg agccggtttc tggtgagtcg tggtctgtgg ctgatcgtcg
     6001 tcgagctgac gctgctgacc gtgggctgga cctacaactt tacctggagc ttcatcgggc
     6061 tgcaggtgat ctgggttatc ggttggtcaa tgatcctgct cgcaggtttg gcctggctcc
     6121 cgctcagagt catcgcgggc atcgggatcg tcttgatagt aggacacaac ctgctggacg
     6181 gcatccatcc ccatccgctg ctggcggcca ttcccgcgac cagcggcgct ctgaacccgt
     6241 ttggtcaggg tcctattgcc ggcgtgatct cgccggaggc aaccccgtgg gactggttgt
     6301 ggagcttcct gcacgtgttc aacccaccga tcgcgtatcc attgatcccc tgggcgggtg
     6361 taatggcggt gggttacgct ttcgggccgc tgatgaaacg gcctccggac gagcgacgga
     6421 ggttctgtct gtggctcggg ctctcattga ccgcggcgtt cgtgctgatt cgctggctga
     6481 acgtctacgg cgacccatcc ccgtggaagc cgcaggcgga cccggtgtac accgtgcttt
     6541 cgtttctcaa caccagcaag tatccgcctt cgctgctgta cctcctcatg acgctggggc
     6601 cggcgctcgt gttgcttgga ctgatggatc gtgcgtccgg gccggtggtt cgcttcttcg
     6661 tggtctacgg gcgggtgccg ttcctctact acgttgtcca cctgtacctc atccatggcc
     6721 tcacactggt cgcggccgcc atcaccgggc aggcggcgcc gggtgatctg cgggtgttct
     6781 tcctgttcta tccgaagaca ttcggattcg ggctggggac ggtgtatgcc atttggattg
     6841 gcgtggtact ggccctctac ccgctgtgct ggtggttcgc gcggctcaag gccagacgca
     6901 aggatgtgtg gctgagttac gtgtaaagcg ggagaaaggg agaagggggg aaggggagaa
     6961 agggtgagct aaggtacctt tctgcctttt tccttttccc ccttttctcc cttcgccctt
     7021 tctcccgctt tgtcgagtat ccggacctca tcgtccaggc ccaacatctc ccagcgctga
     7081 agcgcgatcg ggacgttgcc gttcttccag aggaaatcgt ggaagctccg cagggagaag
     7141 cgatcgccca ggtgttgtcg ggcgtcggtg agaaaggcga gaatctggag cttgccgatt
     7201 tcgtaagtga tggcctgccc gggtgaggcg gcaaagaaag cagcctcagt
//
LOCUS       DASRJQ010000575         2812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2867696, whole genome shotgun sequence.
ACCESSION   DASRJQ010000575 DASRJQ010000000
VERSION     DASRJQ010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2867696"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VFB89_13100"
     CDS             complement(<1..226)
                     /locus_tag="VFB89_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28278.1"
                     /translation="MDRRLGHEWDEWDGKPLPDQGNYDSDPVLFFGWSAATIAAAGVA
                     GALFLYLLGPRLALVHPAVPRTLWLVGGAAL"
     gene            354..2006
                     /locus_tag="VFB89_13105"
     CDS             354..2006
                     /locus_tag="VFB89_13105"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZM28279.1"
                     /translation="MPSEPPVRGPFRTDERARAAYAEAAGIYRILPHAVCLPADIDDL
                     LTLVRWAADERVALVPRGAGSAMGGGNIGDGVMVDLTRMSGPTTEVRPERRRARMRAG
                     ASLGELDTAATAHALRLPPDPSSWRWATAGGVVSTNAAGARSARYGSVRRWVEGLTFV
                     TADAEVGSLSRGRRSQSQPQVALPEAVLRFERETAAAIETARDLVGTRYPRIRKSSSG
                     YALDAYLRSGDALDLVIGSEGTLVLVTEVEWRLDRVPAHRAGLRLLLKSYDTIDELVT
                     RLVALGASAVELLDRTFLELVAAHSKEPAGRAGIEAVLLVELESSDPEVLREATSAAV
                     TAGRQLAYEVDAALSPEGAARLWQLRHAASPILAGLPPDRRSLQVIEDGCVPVPRLGE
                     YIMTIRQAAAARGLSVVIFGHAGDGNVHVNLLPELARPGWERDVAELLEEITDAVISL
                     GGAPSGEHGDGRLRAGFLERLYGTEIVELFRRIKTSFDPLGLLNPGIILPSGEPPISR
                     LKAGAGAIPLPEDIARALRDIEQSGGYAKSRLEIADSPADSR"
     gene            2042..2794
                     /gene="thyX"
                     /locus_tag="VFB89_13110"
     CDS             2042..2794
                     /gene="thyX"
                     /locus_tag="VFB89_13110"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HZM28280.1"
                     /translation="MEIIKRPTVTLIARPQFVEPEHLRVQWKGDSTDGERVAEYAGRL
                     CYMSQRNPAGRSTAEYLQNILRQGHGSVFEHSVYVMLIEGISRSCSHELVRHRAGFGY
                     SQLSQRYVDESHAAFVMPPAVQGDPALEAAWVEQVTAAQAAYVTAVEGLMTKYAWLDD
                     RTHRRKMAREAARSVLPNATEVKIVVSANVRAWRTMLELRLGEGAELEIRRMAIECLR
                     VLQREAPALFADFQIYCAPDGNEAGRVGFHKV"
BASE COUNT          484 a          906 c          948 g          474 t
ORIGIN      
        1 ccagcgccgc tccacctacc agccaaagcg tccgcgggac cgcggggtgc accagagcca
       61 gccgcgggcc caatagataa aggaataggg ctcccgccac tcccgccgcc gcgatcgtgg
      121 cggccgacca cccgaaaaag agcaccgggt ccgagtcgta gttgccctga tcgggcagcg
      181 gcttgccgtc ccactcgtcc cactcgtgac caagacggcg gtccacatcg atgtgaatca
      241 gctgagcccg tggggcgggt gctgcaacgg taggcggggc gtctggcata cggaaacagt
      301 attctctcgt cgcggcgatt gcttggcaag ggagcgcgac cctagctttc cccatgcctt
      361 ccgagccccc ggttcgaggc ccgttccgca ccgacgagcg agcccgggcc gcctatgcag
      421 aggccgcggg catctaccga attctccccc acgcggtctg ccttcccgcc gatatcgatg
      481 atctgctgac gctggtccgc tgggctgccg atgagcgcgt tgcgctggtg ccgcgaggag
      541 cggggagtgc gatgggaggc gggaatatcg gagatggcgt catggtcgat ctgacgcgaa
      601 tgagtggacc gacaactgag gtgcgaccgg agaggcgccg tgctcgcatg cgtgcgggtg
      661 cgtcgctggg cgagctcgac acagcggcga ctgcccatgc gttgcgactc ccgcccgatc
      721 cgtccagctg gcggtgggcg accgcgggcg gcgtagtctc caccaacgcc gcgggcgcca
      781 ggtcggcccg atacggcagc gttcggcgat gggtagaggg actgactttc gtgaccgccg
      841 atgccgaggt tggcagcctc tcgcgggggc ggcgatccca gtcgcagccg caggttgcat
      901 tgcccgaggc ggtactccgc ttcgagcggg agaccgcggc cgctatcgag accgcccgag
      961 acctggtcgg aacccgctat ccccggatcc gcaagagctc ctcgggttat gcactcgatg
     1021 cgtacctaag atcgggcgat gcgctcgatc tggtcatcgg ctcggaaggc acgctcgtgc
     1081 tggttaccga ggtcgagtgg cgcctggatc gggtgccggc acatcgggcc ggccttcgtc
     1141 tgctcctcaa gagctacgac acgatcgacg agctggtcac tcggctggtc gccctgggag
     1201 caagtgcggt ggagctgctc gatcgaacct ttctggagct ggtggcagcg cactcgaagg
     1261 agccggccgg ccgcgccggc atcgaagccg tcctcctggt cgaattggaa agctcggacc
     1321 cggaagtgct ccgcgaagcc accagcgctg ccgtgacagc cgggcggcag cttgcctatg
     1381 aagtggatgc agctctgtct cccgagggtg ctgcgcgcct ctggcagctg cgtcacgccg
     1441 cgagcccgat tctcgcaggc ctgcctcccg accggcgttc gctccaagtc atcgaagacg
     1501 gatgcgtgcc ggtgcctcgc ctcggcgagt acatcatgac catcagacaa gctgcggcgg
     1561 cgcgaggact gtcggttgtg atctttggac atgccggcga cggcaatgtt cacgtcaacc
     1621 tgctgcctga gctggcccgc cccggctggg aacgcgacgt cgccgagctg ctcgaagaaa
     1681 tcaccgacgc cgtgatctcg ctgggaggtg ccccctcagg tgagcacggt gacgggcggc
     1741 tgcgggcggg attcctcgag cggctgtacg gtaccgaaat cgtcgagctg tttcgccgga
     1801 tcaagaccag cttcgacccc cttgggttgc tcaaccccgg cattatcctt ccctccggcg
     1861 aaccgcccat ctcgcgcctc aaagcaggtg ccggcgcgat tccactgccg gaggatatcg
     1921 cgcgggcact tcgggacatc gagcagagcg ggggctacgc caaatcccgg ctcgagatcg
     1981 ctgatagccc agctgatagc cgatagctga tagccgttac caaacgacat tctctaccta
     2041 catggaaatc atcaaacgcc ccaccgtcac tctcatcgcg cgaccccagt tcgtcgagcc
     2101 ggagcacctc cgagtgcaat ggaaaggcga ctcgaccgac ggagagcggg tggcggagta
     2161 tgccggccgg ctttgctaca tgagccagcg aaatccggca ggtcgttcca ccgctgaata
     2221 cctgcagaac atcctgcggc aggggcacgg gtcggtcttc gagcactcgg tctacgtgat
     2281 gctgatcgag gggatctcgc ggtcctgcag ccacgagctg gtccgccatc gcgccgggtt
     2341 cggctactcg cagctttctc agcggtacgt ggacgaatcg catgccgcct ttgtcatgcc
     2401 tccggccgtt cagggcgatc cggcgctgga ggccgcgtgg gtcgagcagg tcacggcagc
     2461 ccaggccgcc tatgtgaccg cagtggaggg gctgatgacc aagtatgcct ggctggatga
     2521 tcgcacccat cgacggaaga tggcccgcga agccgcgaga agcgtcctgc cgaatgccac
     2581 cgaagtgaag atcgtggtga gcgcgaacgt tcgcgcgtgg cgcaccatgc tcgagctgcg
     2641 actgggcgag ggcgcggagc ttgagattcg ccgcatggcg atcgagtgcc tgcgtgttct
     2701 gcagcgtgag gcacccgcgc tcttcgcgga cttccagatc tactgcgcac cggatgggaa
     2761 cgaggcaggg agagtcgggt ttcacaaggt ctgaagaacg tgaacggtga ac
//
LOCUS       DASRJQ010000576         3366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3996069, whole genome shotgun sequence.
ACCESSION   DASRJQ010000576 DASRJQ010000000
VERSION     DASRJQ010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3996069"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            684..893
                     /locus_tag="VFB89_13115"
     CDS             684..893
                     /locus_tag="VFB89_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28281.1"
                     /translation="MTPTDPLLTPPRAISDDERLRQRRAEWREQAVPLDVERLRAEAK
                     RVQEAEQQRKAAMRAEERRMPNGHD"
     gene            886..1092
                     /locus_tag="VFB89_13120"
     CDS             886..1092
                     /locus_tag="VFB89_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28282.1"
                     /translation="MTDDRERRCLQCGEPLPPGRLVFCRPSCRAKYEHQHYQPTLFPE
                     PLDFDAAWPEDERVRPRANRRRER"
     gene            1112..1357
                     /locus_tag="VFB89_13125"
     CDS             1112..1357
                     /locus_tag="VFB89_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28283.1"
                     /translation="MPELRLEPPGGIRSGAPRLRLQLLLDDMEAAPAPRTAGEEARGA
                     ATEREALMTKHTGLSTRRSAARDLARARCGERGDVYG"
     gene            1675..2184
                     /locus_tag="VFB89_13130"
     CDS             1675..2184
                     /locus_tag="VFB89_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28284.1"
                     /translation="MPSTHAQQAVQAVDDYIELRAKWEVLADSGALTEDADEALRDQL
                     DAAITRIIEACAADLGQRSALDVARRAQLWSEATFNAGCLFSSRMRALGMPMLTRHEL
                     DPRTEKDADPCPCEDVQLSPRCHPHATVRASYDGQQGRLRLRCAACDRHFANIAVEPG
                     GEAMQRRVN"
     gene            2190..2417
                     /locus_tag="VFB89_13135"
     CDS             2190..2417
                     /locus_tag="VFB89_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28285.1"
                     /translation="MPRGLGFRQWAILHELKREGGALWLRNLDASAHRNLLPSLCRAV
                     KGLERRGHLRIVKQAPTGWRRKLWLLDLLAR"
     gene            2840..3070
                     /locus_tag="VFB89_13140"
     CDS             2840..3070
                     /locus_tag="VFB89_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28286.1"
                     /translation="MEVTYAVTFEFETRAPLTHRGTIGGSSAATCVARATREAQKALR
                     PVAWSSMVCCLLERRDGAAAPEPTEGGNHEGL"
     gene            3057..3347
                     /locus_tag="VFB89_13145"
     CDS             3057..3347
                     /locus_tag="VFB89_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28287.1"
                     /translation="MKDSDRMHSQEEMEELVRTGAECGYSKVEVEALVEEGEQSRSFV
                     RFENGEPCFYTPLTECFPDEAELEEVCRAADEAGMPAGTFVMDCLRKYLAVH"
BASE COUNT          581 a         1117 c         1196 g          472 t
ORIGIN      
        1 gcgaggcccg accggagatc aaggcggcaa ggcgtcgggc gacgggttcc ggagctagga
       61 gcgtcagcga accctgcacg gtggtgcagg gttccgagag cgtcaaccct gcacggcggt
      121 gcagggttcc gaccgatcta accctgcagc gcggtgccga taaacatgca cccccgtgca
      181 ccgaaccctg caccccggtg ccgtcaaccc tgcaccccgg tgcacctgat cttcatgatc
      241 ttcatgatct gaaaagaagt accggcaccg gcgctgacgc gccggggttt tcccaccgag
      301 ttgaaaagac caagagcccg agcagcgcag cggacgagcc acgcctgagc gaggccgagc
      361 cgcagcggcc cgacgacccc gaccccacgg tcattcgcgg tgtcggacgt ggagagttag
      421 tactaactct caactccgat tcagtcgctc gctcctcaac gagcgagacc acggaaaggc
      481 cccttgagta ctcaagggcc ccgtccgaag ccattgagta ctcaatggcc ccgtccgaag
      541 ccatcaccgc cgccgccctc gagccgccgc cgccgccgcg cggcgcgccg cgggaggctc
      601 acgctgaacc gccacaggct cctgacgcgg acgcgcatgc cgatgcggac gccgcggagc
      661 ctcgctcaga aggagacagt gccatgacgc caaccgatcc gcttctcacc cctccccgcg
      721 cgatctccga cgacgagcgc ctgcgccagc gccgcgccga atggcgcgag caggccgtgc
      781 cgctcgatgt cgagcggctg cgcgccgagg ccaagcgcgt gcaggaggcc gagcagcaac
      841 gaaaagccgc gatgcgcgcc gaggagcggc gcatgcccaa cggccatgac tgacgaccgc
      901 gagcggcgct gcctgcagtg cggcgagccg ctgccgccgg gccggttggt gttctgccgc
      961 ccgtcgtgcc gcgcgaagta cgagcaccag cactaccagc cgacgctctt cccggagccg
     1021 ctcgacttcg acgccgcatg gccggaggac gagcgcgtga ggccgcgcgc gaaccggagg
     1081 cgcgagcgat gaccgacgaa cgagatcgcg agtgcccgag ctgcgactcg agcctcccgg
     1141 cggaattcga tccggtgctc cgcgtcttcg tctgcagctg ctgctcgacg acatggaagc
     1201 cgcgcccgcg ccgcgaaccg ccggagaaga agcgcgcggc gccgcaacgg aacgggaggc
     1261 cctcatgacc aagcacacgg gcctgagcac gcgccggagc gctgctcggg atctcgcgcg
     1321 ggcgaggtgc ggcgaacgag gagatgtgta tggatagaga gacatcaacg ccgcgttgta
     1381 ttagttgcgg tcatccgttc gagagcgaca gcgatcggta tccacggttc cccggcgact
     1441 tcctgagcct ctgcggcgcc tgcgcgggcc ggctgcgtgc ggcggggagt ttcgcggctt
     1501 ggctcgatgc ggaggacctc cgcagaacgc cggtgcctgg cgtgcactga cgcgcgcgcg
     1561 agaaccgatc tcgcgaaggg agcgagcgat gaagagcgaa tgccggacgc tcagtccgga
     1621 gttgccgatc cacctttgac ctcggtgcac tgattcgacg aacggagggc cacgatgcca
     1681 tcgacacatg cacaacaagc cgtacaagcc gttgacgact acattgaact gcgcgcgaag
     1741 tgggaggtcc tcgccgacag cggtgcgctc acggaggatg ccgacgaggc actcagagac
     1801 caactcgacg cagccatcac ccgcatcatc gaagcctgtg cggcagatct cggtcagcga
     1861 tccgcgctcg atgtcgctcg gcgcgcccag ctgtggtctg aggcgacgtt caacgccggg
     1921 tgcctgtttt cgtcgcgcat gcgtgctctc gggatgccga tgttgactcg gcatgagttg
     1981 gatccgcgca ccgagaaaga cgcggacccg tgcccctgcg aagacgtgca gctctcaccc
     2041 cgttgtcatc cacacgcgac cgtccgcgcc tcgtatgacg gacagcaggg gcggctccga
     2101 ctccgctgtg cggcgtgcga taggcacttc gccaacatcg ccgtcgaacc cggtggcgag
     2161 gcaatgcagc ggcgcgtgaa ctgaggccga tgcctcgcgg gctcggcttc cggcagtggg
     2221 cgattctgca cgagctgaag cgcgaaggcg gcgcgctctg gcttcgcaat ctggacgcca
     2281 gcgcccaccg caacctgctg ccctcgctct gccgcgcggt gaagggcttg gagcgccgcg
     2341 gccatctccg gatcgtgaag caggcgccca cgggctggcg ccggaagctc tggctcctcg
     2401 atctgcttgc ccgatagacg ggtcgattcg gggtcgtgca ggggcggtcc tgggctcggc
     2461 cgcgggtgcg tgcgaggttc tccggcggga attcgcgagg ccgcgcggcg gcgctgcggt
     2521 acggcccgtc aggcctataa acgcgacctc gcacagacgg gcagcacgcg ccctcctgag
     2581 tcgcgagccc aaaacttcgg aaggcccccc cggcaagagg gcccccggct ggcgcagcag
     2641 cgcgaccacg aggcgttcgc ccgcgcgcgc gggctccagt acgcgggacg cgaggctcgc
     2701 gctgaggggc gataacgggc tcggcggttc gttcgatctc ggagagtccg aggttgacag
     2761 gttgggtcag cagctcggcc gcgccagcgg gcgcaccagc gggcgcaggc gcgaggccga
     2821 gcggagggga ggcgcaggca tggaggtgac gtatgccgtc acgtttgaat tcgagacgcg
     2881 ggcgccgctg acgcacagag gcaccattgg cggctccagt gcggccacat gtgtggcgcg
     2941 ggcgacgcgg gaggcgcaga aggcgctgcg gcccgtcgct tggtcgtcga tggtctgttg
     3001 tctgctggag cgccgcgatg gggccgcggc gcccgagccg accgagggag ggaaccatga
     3061 aggactctga ccgcatgcac tcgcaggagg agatggagga gctggttcga acgggcgcgg
     3121 agtgcggcta ctcgaaggtc gaggtcgagg ccttggttga ggagggcgag cagagccggt
     3181 cgttcgtgag gttcgagaac ggcgaaccct gcttctatac gccgctgacc gagtgcttcc
     3241 cggacgaggc cgaactggag gaggtgtgcc gcgccgccga cgaggccggg atgcccgccg
     3301 ggaccttcgt catggattgc ttgaggaagt atctggcggt gcactgagcg ccggagcgtg
     3361 agcagc
//
LOCUS       DASRJQ010000577         3440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2626817, whole genome shotgun sequence.
ACCESSION   DASRJQ010000577 DASRJQ010000000
VERSION     DASRJQ010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2626817"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VFB89_13150"
     CDS             complement(<1..573)
                     /locus_tag="VFB89_13150"
                     /inference="COORDINATES: protein motif:HMM:NF014056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF99 family protein"
                     /protein_id="HZM28288.1"
                     /translation="MANHGRQLISRPHVLGIDDAPFRKGRDQSVPIVGVVMEGPDLIE
                     AVAVATFAVDGDDAAGFLSAWVRGLRAYRSIQAILLGGITIAGLGVVDLDKLAEVVSR
                     PVVAVTRRNPAGSELRRALRAAGLTERLALLDRSPAAIEAAPGLFVAGSGLSPAEAVA
                     LVRRTLRKSRLPEPLRVAHMVAGALATGQSR"
     gene            complement(586..1380)
                     /locus_tag="VFB89_13155"
     CDS             complement(586..1380)
                     /locus_tag="VFB89_13155"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HZM28289.1"
                     /translation="MTSIQVDPSKQPLVDLHNHLIPAIDDGARTLAEAIDCLRALALD
                     GVGTVVATPHLLLPQLPTREAVEKELARHRRAFETLETAVEQQPGLPELGLGQEIWAS
                     SAEMIAPVLGCEGLGLAGTPYLLIEFGFDLQGTHNDVVEEVLERGRKIVIAHAERYRF
                     PDGLDPLETAADWREAGALLQVNTGSFTGYYETSNPEAQTLAWQLLEAGLIDLIATDH
                     HGSRRSRGSPREAYDALVAAGYASQAERLLGTNPASLLAAAGARTE"
     gene            complement(1413..2339)
                     /locus_tag="VFB89_13160"
     CDS             complement(1413..2339)
                     /locus_tag="VFB89_13160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HZM28290.1"
                     /translation="MISVLRAPPFATVQDLGRPNHLDAAVPPSGALDRFALAAGNLLL
                     GNPRDAAGLEWGLGGGKLRFEQATAFLLLGAHGEATLGDRAVERGVLVQAAGGEVLEV
                     HRLERSAWLYLAVAGGIDIPLVLGSRSTYLPARFGGFEGRTLRSGDRLPIGAAPLTHT
                     STNAPSVLLESDDQPIRILPGPDIDMLEPGDWERFVEAEYHISRAASRMGYRLEAGGS
                     RVRVTQDRPSAPVCVGTIQLPAGGEPIVLLADGPTVGGYARIGVVATADVGRLAQRRP
                     GDPVRFRVIGLEEATQILREQAGLLNRLEEGA"
     gene            complement(2336..2995)
                     /gene="pxpB"
                     /locus_tag="VFB89_13165"
     CDS             complement(2336..2995)
                     /gene="pxpB"
                     /locus_tag="VFB89_13165"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HZM28291.1"
                     /translation="MTYSTRIVPLGDSAYLVSLGTGVDLELSARIARLANELRGREVA
                     GRIAVVPAYAALAVHFDPAIADASALADLLRRLAAAELQQPAPDLGKTGKCHVIPVRY
                     DGPDLDTVAQRLQLPPAEVIRRHAERTYQVLFLGFVPGFGYLGPLDPTLSLPRRPEPR
                     QRIPPGTVAIAAEQTAIYPIETPGGWHLIGRTDTVLFDPSRSVPNLLAAGDRVRFEPI
                     P"
     gene            complement(2992..>3440)
                     /locus_tag="VFB89_13170"
     CDS             complement(2992..>3440)
                     /locus_tag="VFB89_13170"
                     /inference="COORDINATES: protein motif:HMM:NF015691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HZM28292.1"
                     /translation="VRYVKPHGALYNRAVRDPAIAKAIADGVRAADSGLALLGLAGSA
                     LLDAAAETGLRPIAEAFLDRGYRKDGSLVPRDQPGALIDDAAIAAARAVRLVTEREVE
                     TTDGSIIEVAAESLCVHGDDARAVIVLRAVRKSLASAGIAVAPFLR"
BASE COUNT          631 a         1123 c         1119 g          567 t
ORIGIN      
        1 gcgcgactgg ccggtggcga gcgcgcccgc caccatgtga gcgacccgga gcggctcggg
       61 cagacgtgat tttcgcagcg tacgccggac cagcgctacc gcttctgcgg gactcaatcc
      121 ggacccagca acgaaaaggc ccggggccgc ctcgatcgcg gctggcgatc gatccagcaa
      181 tgccaggcgc tcggtcagtc ccgcggctcg cagcgcgcgc ctcagctccg aaccggcggg
      241 gttgcgccgg gtgacggcga cgaccggccg cgagaccact tcagccaact tgtccagatc
      301 gaccacgccg agccccgcga tggtgatccc tccgagcaaa atcgcctgga tgctgcgata
      361 ggcgcgaagc cctctaaccc aggcgctcag aaatccggca gcgtcgtcgc cgtccacggc
      421 aaaggtcgcc accgcgactg cctcgatgag atcgggccct tccatgacga ccccgacaat
      481 aggcaccgat tgatctctgc ccttgcgaaa cggggcgtca tcgatgccca gcacgtgggg
      541 tcgcgaaatg agttgtcggc cgtggttagc cactctggcc cgatgttact ccgtccgtgc
      601 gccggctgcc gcgagcagac tcgcgggatt tgtgccgagg agccgctccg cttgtgaagc
      661 gtagccggcg gctaccagcg catcgtatgc ctctctgggt gatccgcgcg atcggcgcga
      721 tccgtgatgg tccgttgcga tgaggtcgat cagaccggcc tcgagcagct gccaggcgag
      781 cgtctgagcc tcgggattcg aggtctcgta gtaacccgta aagcttccgg tattcacctg
      841 gaggagagca cctgcctcac gccagtcggc agccgtttcc aacgggtcga gtccgtccgg
      901 gaaacgatat cgctcggcgt gagcgatgac gatcttgcgg cctcgctcga ggacctcttc
      961 aaccacgtcg ttgtgagttc cctggagatc gaatccaaac tcgatgagca gataaggagt
     1021 gcctgccaga cccagccctt cacagccgag caccggcgca atcatctcgg cggaagaggc
     1081 ccagatctcc tggccgagcc cgagctcggg cagtcccggc tgttgctcga cggcggtctc
     1141 gagtgtctcg aaagcacgac gatgcctggc caattccttc tccaccgcct cgcgcgtggg
     1201 caactgggga agcagcagat ggggcgtcgc cacgaccgta cctacgccat cgagcgccaa
     1261 tgccctgagg cagtcgattg cctcggcgag cgttctcgcg ccgtcgtcga ttgccggaat
     1321 caggtgattg tggagatcta ccaggggctg cttggaggga tcgacctgta tgctggtcat
     1381 tttggtcata acggcgcgaa gtttagcaac ctttatgccc cttcctccag ccgattcagc
     1441 aaccccgcct gttctcgcaa gatttgcgtt gcctcctcca acccgatcac tcggaagcga
     1501 acgggatcgc caggtcgacg ttgagccagg cggccgacat cagccgtcgc caccacgccg
     1561 attctggcgt agcctcctac ggtaggtccg tccgcaagca acacgatcgg ctcaccaccg
     1621 gccggcagct gaatggtgcc gacacatacg ggcgcggagg ggcgatcctg ggtgactcga
     1681 acacgggaac cacctgcctc cagccgatag cccatgcggc tggcggcgcg cgagatgtga
     1741 tactcagctt ccacgaatcg ctcccaatca ccgggctcga gcatgtcgat gtccggcccc
     1801 ggcaagattc tgattggttg gtcgtccgac tcgaggagaa ccgagggcgc attcgtcgag
     1861 gtgtgtgtga gcggtgcagc tccgatcggt agtctatcac ccgagcgcaa agtccgacct
     1921 tcgaagccgc caaaacgagc cgggagataa gtggaccggc ttcccagcac cagtggaata
     1981 tcgattcccc cggccacggc gagatagagc caggcacttc gctcgagacg gtgaacctcg
     2041 aggacttccc ctcccgcagc ttggacgagg acacctcgct cgaccgcacg gtcgcccaga
     2101 gtggcctcac catgcgcgcc caggagcagg aacgccgtcg cttgctcgaa gcgaagtttt
     2161 ccgccgccga ggccccattc caatccggcg gcgtcccgcg ggttgcccag caggagattg
     2221 ccggccgcaa gggcaaaacg atcgagtgca ccggacggcg gcacggcggc atcgagatga
     2281 ttcggtctgc cgaggtcctg cacggtcgcg aaaggcggag cgcgaaggac gctgatcacg
     2341 gtatcggttc gaatcggacg cggtcgcccg cggcgagcag gttaggcacg ctccggctcg
     2401 gatcgaacag gaccgtgtcc gtgcgcccga tcaggtgcca gccacccggg gtttcgatcg
     2461 gatagatcgc ggtctgctcg gcggcaatag caacggtccc cgggggaatc cgttggcgcg
     2521 gttccggcct tctcggcaaa gaaagcgtgg gatcgagggg gccgagataa ccgaaacccg
     2581 gaacgaatcc gaggaacagc acctggtagg tgcgctctgc gtgccgtcgg atgacctccg
     2641 cgggcggcaa ctgcagccgc tgcgcgaccg tgtcgagatc cgggccgtca tagcggacgg
     2701 gaatgacgtg acatttgccg gtcttgccaa gatcaggggc cggttgctgc agctcggccg
     2761 cggccagcct gcgcagcaag tccgccaggg cacttgcatc ggcgatcgca ggatcgaaat
     2821 gaactgccag cgccgcgtag gccggtacga cggcgattcg cccggcaact tcgcgcccgc
     2881 gcagctcgtt cgcgagcctt gcgatgcgag cgctcagctc gaggtcgaca ccggtgccga
     2941 ggctgacgag atatgccgaa tcgcctaggg ggacgatccg cgtcgagtag gtcaccggag
     3001 aaacggtgcg accgcgattc ccgccgacgc gaggctcttc cgtaccgcgc gcaacactat
     3061 gacagcacgc gcgtcgtcac cgtgaacgca caatgactcg gcggcaacct cgatgatcga
     3121 gccatccgtg gtttcgactt cgcgttcagt gaccaggcgg accgctcgcg ctgctgcaat
     3181 cgctgcgtcg tcgatgagcg cgcccggttg gtcgcgggga accagagagc cgtctttgcg
     3241 atagccgcgg tcgagaaaag cctccgcgat cgggcgcaga ccggtttcgg cagcagcatc
     3301 gagcagcgcg ctgccggcca gtcccaggag ggcgagaccc gagtcggctg ctcgaacgcc
     3361 gtcggcaatg gctttggcga tggcggggtc ccggacggcg cgattataga gagcgccatg
     3421 tggcttcaca taccgtacgc
//
LOCUS       DASRJQ010000578         2281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1257495, whole genome shotgun sequence.
ACCESSION   DASRJQ010000578 DASRJQ010000000
VERSION     DASRJQ010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257495"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VFB89_13175"
     CDS             complement(<1..655)
                     /locus_tag="VFB89_13175"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZM28293.1"
                     /translation="MPLSVVVVGAGAFGGWTALHLLRTGARVTLIDSWGPGNSRASSG
                     GDTRVIRGIYGGNRRYVELTARSFALWWEHEARWQRRLYHRTGALWMFTGDDTYARST
                     RPILREIGLEVAELTPTEAESRFPQIDFTGIRTAFYEIEAGYLLARQACETVRDGFVA
                     EGGDYRQVGAEVGPISGRSLNELKLSDGTRLPADRFVFACGPWLGQLFPDAVGERISP
                     "
     gene            complement(656..2128)
                     /locus_tag="VFB89_13180"
     CDS             complement(656..2128)
                     /locus_tag="VFB89_13180"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZM28294.1"
                     /translation="MSPPEPAAALPELPRTLTLRDLVLLNVVAVLSIRWLATSAAAGP
                     SSLVLWVCAALFFFVPQGLAVADLAARFPDEGGIYAWTKRAFGEGHGFLCGWCYWVNN
                     VLYYPSLLLSGAVIATYAFGAGGSGLGERWTYVLPVTLGAMWLAVWLNVVGLNIGKWL
                     QNLGAIGSYVPGIIIVVLGVVAVVTRPPVNTFTPATLTPDLSKIPELNLWASIAFAFA
                     GLELSATLGGEIRDPRRMLPRSIFLAAPLIALIYIAGTGAILWMVPQKNVNLVSGLLQ
                     GIQSGAQGLGPIVAWAVPFAAAANALGNLGCVGAWLTGCARVAFVVGLDRYVPPAFGR
                     IHPKWGTPYIAILVQAALSTIFLFISVVGKGTTVERAYLILLDTMILVYFIPYCYMFL
                     TFLVVGLGRRDAGLDQGRAPRRLRTVLVGGTGLTVTVFAMVVAMIPPPGTTDPWLFEL
                     KVAGGAFAFVAAGGLLYWRAVRAAARNPARMATARAGQEA"
BASE COUNT          491 a          744 c          720 g          326 t
ORIGIN      
        1 tgggtgaaat ccgctctccc acggcgtccg ggaacaactg gcccagccac ggtccacagg
       61 caaagacgaa tcgatcagcc gggagccggg tgccgtcaga cagcttgagc tcgttaaggc
      121 tgcgacctga aatcgggccg acctccgcac cgacctgccg gtagtcgccg ccttctgcga
      181 cgaagccgtc gcgcacagtc tcacaggcct ggcgagccag caagtatccc gcttcgattt
      241 catagaaggc agtacggatg ccggtgaaat caatctgggg aaagcgggac tcggcttcgg
      301 tcggcgtcag ctcggcaacc tcgagcccga tctctcgcag gatcggacga gtcgatcgcg
      361 cgtaggtgtc gtcgccggtg aacatccaca acgcgccggt gcggtggtac aaacgtcgct
      421 gccagcgtgc ttcgtgctcc caccagaggg cgaaggaccg ggcggtgagc tcgacgtagc
      481 gtcggttacc gccgtagatc ccccggatta cccgagtgtc gccacccgag ctggctcggc
      541 tgttcccggg accccagctg tcgatcaagg tgactctcgc cccggtccga agtagatgaa
      601 gtgcggtcca gcctccgaac gcgccggccc cgactaccac aaccgagagc ggcattcagg
      661 cttcctgacc ggcccgcgcg gtcgccattc gagctgggtt cctggcagcg gcccggaccg
      721 cgcgccaata gagcagccca ccggcggcaa cgaatgcaaa agcaccgccc gcgaccttca
      781 gctcgaagag ccagggatcc gtggttccgg gaggcggaat catcgcgacc accatggcaa
      841 acaccgtgac tgtgagaccg gtcccgccga ccagcacggt gcgcaatcgc cgcggtgcac
      901 gaccctggtc cagtccggca tcacgccggc ccaggccaac gaccaggaac gtgagaaaca
      961 tgtagcagta ggggatgaag tagaccagaa tcatcgtgtc gagcaggatc agatacgccc
     1021 tctcgacggt cgtccccttc cctaccaccg agatgaacag gaaaatcgtg gacagcgccg
     1081 cctgaaccag aatcgcgatg tacggagtgc cccatttggg gtggatccga ccaaaggcgg
     1141 gcggaacgta tcggtcgagt cctaccacga atgcgacccg agcgcagccg gtcagccatg
     1201 cgcccacgca accgagattc cccagcgcgt tcgccgctgc cgcaaacggg acagcccagg
     1261 caacgatcgg ccccagtcct tgcgccccgg attggattcc ctggagcaga cccgacacca
     1321 ggttcacgtt cttttggggc accatccaga gaatggcacc ggtaccggcg atgtagatga
     1381 gggcgatcag tggggccgcg agaaaaatcg atcgcggcag catgcggcgc ggatcgcgaa
     1441 tttcaccgcc aagggtagcc gacaactcga gcccagcaaa tgcaaaggct atggacgccc
     1501 agaggttgag ctccgggatc ttgctgaggt cgggagtcag cgtagccggg gtaaaggtgt
     1561 tcaccggtgg gcgagtcacg acggcgacaa cgccgagcac gacgataatg atcccgggaa
     1621 cgtaagaccc gatcgcccca aggttctgaa gccatttccc gatgttaagc cctaccacgt
     1681 tgagccatac agcgagccac attgcgccga gcgtcaccgg caggacgtag gtccaacgct
     1741 cgcccagccc actcccacct gcgccaaacg cataggtcgc gatgacggcg ccggagagta
     1801 gcagactcgg gtagtacagc acgttgttaa cccaatagca ccacccgcag agaaagccgt
     1861 gcccctcgcc aaaggcacgc ttggtccagg cgtaaatgcc gccttcgtcg ggaaagcgcg
     1921 ccgccaggtc ggcgaccgcc aggccttggg gcacgaaaaa gaacagcgcc gcgcagaccc
     1981 agagcaccag gctcgatggt ccggcggcgg cagacgtggc gagccagcga atgctgagca
     2041 ctgccacgac attgagcaga acgaggtcgc gcagggtgag agtgcgcgga agctccggca
     2101 atgcggctgc aggctccggt ggactcatcc tttgagatag agccgctgcg gatccagaac
     2161 gtcgcgaagg aggtgaagtg ctcgcgcgtc cggcgcctcg acacaaccga gctgatcccg
     2221 agcccggagg ggccagccca cgccggcacg aacctcgtcg agggtgactc caggatagac
     2281 c
//
LOCUS       DASRJQ010000579         2348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2077585, whole genome shotgun sequence.
ACCESSION   DASRJQ010000579 DASRJQ010000000
VERSION     DASRJQ010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2077585"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2348)
                     /locus_tag="VFB89_13185"
     CDS             complement(<1..>2348)
                     /locus_tag="VFB89_13185"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HZM28295.1"
                     /translation="RSPPAGRDRLRAVLSTVVTTLFVISVPLAAQLEPPSTGGVVALE
                     HELRLLGHYKRVLVIGAHPDDEDTELLTVLVRGEGAEAAYLSLNRGEGGQNAIGPELG
                     EPLGLLRTEELLAARRLDGARQFFTRAYDFGFSKSLDETWTHWPRDSVLKDVVRVIRR
                     FRPQIVVSVFSGTPRDGHGQHQAAGWAAQEAFRIAGDATRFPELARDQLAPWMPLKLY
                     RSTRFDSAATTLVLQGGVLDSAVGKSYHQVAMASRSLHRSQDMGQLQRIGPSVVRLAL
                     IQDRTGKGAGGLWAGIDTTLAGLASAGGRAPASGPWDSLARAIADYRRTGGPPLAATL
                     DRFAAALPQAESQGTIKVARASAEQWDQLARMQHAIQLASGMVCDAVADDQRIVPGQT
                     LHVDATCWNAGSRPISLPLVAIRGNSTWPGVRETKEGATVQPGAVRTWAFDVPIPDTA
                     NPTTPYFLQTPRQGDFYVWPAGPPDLLGLPFEEPLLTADFGAEIAPREVTFQYRDQTT
                     GEARRPLSVVPRIDVKLDPDTLVWATSSRGARRFAVTLVNGARDTTTGSLRLVVPSGW
                     RTSRVVSFTLSREDESRSYSFDVTPPATVRPGTFTIRAEATDRLGARYDTGVFRVDYP
                     HVRPRSFTRPAVASLRVAPLALPRLARVGYVRGAADRVPEALQSVGIPVSLLDGDALE
                     RENLSRYAAIVVGSRAYETEPALQRFNRRLLEYVRRGGLLLVQYQQYAFFLAGYAPYP
                     MTVAGQALRLEAAPATGTRATPPRPAGPPVSHDRVTDESAPI"
BASE COUNT          434 a          765 c          786 g          363 t
ORIGIN      
        1 tgaatcgggg cggattcgtc ggtcacccga tcgtgactca ccgggggacc cgccgggcgg
       61 ggcggcgttg cgcgggtgcc ggtggcgggg gcagcctcga gccgcagcgc ctggcccgcc
      121 actgtcatgg ggtagggcgc gtaccctgcc aagaagaacg catactgctg gtactgcacc
      181 aggagcaggc caccgcggcg aacgtactcg agcaaccggc ggttgaagcg ttggagcgcg
      241 ggctcggtct cgtatgcccg actgcccacg acgatggcgg cgtagcggga aagattttct
      301 cgctcgagtg cgtctccatc gagcaggctg accggtatgc caacgctctg gagcgcttcc
      361 ggtacccgat ccgctgcacc cctcacatat cccactcgcg ccaaccgcgg cagcgccagc
      421 ggagccaccc gcagagaagc aacggcaggt cgagtgaagg accgagggcg aacgtgggga
      481 tagtcgactc tgaaaacgcc cgtgtcgtag cgcgccccca accggtcggt cgcctcggcc
      541 cggatggtga aggtgcctgg ccgcaccgtc gcgggaggcg tcacgtcgaa gctgtaggag
      601 cgagactcgt cctcgcgaga tagggtaaaa gaaaccacac gagaagtcct ccaaccgctc
      661 ggcacgacca gtcgcaggct gcctgtcgta gtatcgcgtg cgccattcac gagcgtcacc
      721 gcaaaccggc gagcacctcg cgaactcgtc gcccagacca gggtgtcggg atcgagcttc
      781 acatcgattc tcgggaccac agacaaggga cgacgcgctt ccccggttgt ctgatcccga
      841 tactggaagg tgacctcccg aggcgcgatc tcggctccga aatccgcggt gagcagcggc
      901 tcttcgaacg gcagcccaag aaggtcagga ggaccggcgg gccagacgta gaagtcaccc
      961 tggcgcggcg tctgaaggaa atacggcgtc gttggattgg cggtatccgg aatgggcacg
     1021 tcgaaggccc aggtcctgac agcgccgggc tgcacggtcg cgccctcttt cgtctcgcgt
     1081 actcccggcc atgtcgagtt gccgcgaatg gcgaccagtg gaagagatat tgggcggctg
     1141 ccggcattcc agcaggtggc atcgacgtgc agggtctggc ccgggacaat ccgctggtcg
     1201 tccgcgaccg catcgcagac catgcccgag gccagttgga tcgcgtgctg catgcgggcg
     1261 agctggtccc attgctcggc gctggcgcgc gcgactttga tcgttccttg cgactccgcc
     1321 tgcggcaatg cggcagcgaa gcgatccagt gtagctgcga gcggcgggcc gcccgtccga
     1381 cggtaatccg cgatcgccct ggcgagcgaa tcccatggtc cggatgccgg tgcccggcct
     1441 cccgccgacg caagaccagc gagcgtggtg tcaatgccgg cccatagacc acccgctccc
     1501 tttccggtgc ggtcctgaat cagcgccaat cgtacaaccg aagggccgat tcgctggagc
     1561 tgccccatgt cctgcgagcg atgcaggctc cggctcgcca tcgccacctg gtggtacgac
     1621 ttgcccaccg ccgaatcgag cacgccaccc tggagcacga gtgtggtcgc cgcgctgtcg
     1681 aatcgagtgc tgcggtacag cttgagaggc atccatggcg ccaactggtc gcgcgcaagc
     1741 tcgggaaagc gggttgcgtc tccagcaata cgaaaggctt cctgagccgc ccagccggcc
     1801 gcctgatgct gaccgtgacc gtcgcgcggc gtgccactga agactgagac gacgatctgc
     1861 gggcgaaatc gccggatcac ccggaccaca tccttaagaa ccgaatcgcg cggccaatga
     1921 gtccaggttt cgtcgagcga cttggagaag ccgaagtcgt acgcccgggt gaagaactgt
     1981 cgggcaccgt ccagacgacg ggcggccagg agctcctcgg tccgcagcag gccgagcggc
     2041 tcccccaact ccgggccgat cgcattctgg ccaccctctc ctcgattcag cgagaggtac
     2101 gcagcttcgg cgccttcacc ccgaaccaga acggtgagca gctcggtgtc ctcgtcgtcg
     2161 gggtgagcgc cgatcaccaa tacacgtttg tagtggccca gtaaccgaag ctcgtgctcc
     2221 aatgccacga ctccgccggt agagggcggc tcgagttgag cagcgagcgg aacggagatg
     2281 acgaagagcg tggtgacgac agtggagaga acggcgcgca ggcgatcccg tccggcagga
     2341 ggagatcg
//
LOCUS       DASRJQ010000580         2979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2544139, whole genome shotgun sequence.
ACCESSION   DASRJQ010000580 DASRJQ010000000
VERSION     DASRJQ010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2544139"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /locus_tag="VFB89_13190"
     CDS             <1..1200
                     /locus_tag="VFB89_13190"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM28296.1"
                     /translation="KNLAAYDAFLKGNEAAGGFITATPADLRRAVAYYERAVALDSTF
                     VLAWAQLSRAHAFMSPPTPADRAAGRAAVDRARTLAPAAPATYLALGDYYSNVERDWT
                     RANEQYDLGRKVAPNDAELLNGVALVERSQGHFEQSMASLRQALALDPRSVGTARRLA
                     QALLWLKRYPEAMAAADRALALDPESPDLNETKAMVFLAQGDLAGARGVLQAASARAD
                     PVTLAAYVATYWDLFWVLDETQQQLLLRLTPEPFGGDVSNWAICLAQTYAVRGDLVRA
                     RAYSDSSRIAGEALLKEAPDDAAHRINVALAYAYMGRKADAVREAEKATSETPRAKDY
                     YQGTYYLHQLARVYVLVGEPDKAIALLEQVLASPYYLSPAWLRIDPTLEPLRKNPRFQ
                     KLVEGTA"
     gene            1197..>2979
                     /locus_tag="VFB89_13195"
     CDS             1197..>2979
                     /locus_tag="VFB89_13195"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZM28297.1"
                     /translation="MSADLRAALETGLAGRYAIERELGRGGMAMVYLARDLRHDRPVA
                     LKVLHPELGAALGGDRFQREIRLAARLQHPHILTVHDSGEIASASGPPLLWFTMPFVD
                     GETLRARLQRERQLSVEEALTIAREAADALDYAHQQGIVHRDIKPENILLTARHALVA
                     DFGVARALAGAEQGLTETGMTLGTPAYMSPEQASGERSIDGRTDIYSLGSVLYEMLAG
                     QPPFSGASAQAIIAKRFTETAPPVRRSRESVSPEIDAALTRALARLPADRFATAGEFA
                     KALTVQATSPAVPTALAPDRPSATTPPVGAVLSSERQAAAVSTMAGHHRFRVPAWLAF
                     VLGLLVTASMGMLIWQRSHRAGEPAVAGPKRLAVVPFENLGAPEDEYFADGMTDEVRG
                     KLASVSGLQVIANRSVAEYKRSTKSYQQIGQELGVQYLLIGKVRWEKQAGAQSRVRVS
                     PELIDVSSSSTKWQQPFDAALTDVFQVQGEIAGKVARALDVALGEHERERIEVRPTQN
                     LAAYDAFLRGEEASDRMSTTDPPALSRAASAYEQAVALDSTFALAWARLSQARSNIYG
                     NGVITAVGIEQARRAAERALALAPDRPE"
BASE COUNT          502 a          994 c          984 g          499 t
ORIGIN      
        1 aaaaatctgg cggcctacga tgccttcctc aaaggcaacg aggcggccgg gggcttcatt
       61 acggcaacgc cggcggatct gcggcgcgcg gttgcctatt acgagcgggc cgtcgcactc
      121 gactcgactt tcgtattggc atgggcgcag ttatcccggg cccatgcctt catgtccccg
      181 cccactccgg ccgatcgtgc ggccggccgt gcggcagtcg accgggcgcg gacgctcgcg
      241 ccggccgccc ctgcgacgta tctcgcgctc ggtgattact acagcaacgt ggagagagat
      301 tggactcgcg caaacgagca gtacgacctc ggtcgaaagg tagcgccgaa cgacgccgag
      361 ctgctcaacg gcgttgcgct ggtcgagcgg tctcaggggc attttgaaca gagcatggcc
      421 tcactccggc aggccctcgc gctcgatccg cggtcggtcg ggacagcacg ccggctggcg
      481 caggcgctgc tgtggctcaa acgatatccc gaggccatgg ccgccgccga ccgggccctc
      541 gcgctcgatc cggaatcccc cgatctcaat gaaaccaagg ccatggtctt cctggcgcag
      601 ggtgatctgg cgggtgcgcg tggggtgctg caggcagcct ctgctcgcgc tgatcccgtc
      661 accttggcgg cctacgtcgc tacctactgg gatctcttct gggtcctcga cgagacccag
      721 cagcagttgt tgctccggct gacaccagag ccgttcggcg gcgacgtatc caactgggca
      781 atctgcctgg cccagaccta cgccgttcgg ggtgatctgg tccgggctcg tgcgtactcc
      841 gactcatcgc ggatcgccgg cgaggccttg ttaaaggagg cccctgatga tgccgcgcac
      901 cgcattaacg tagcgctggc gtacgcttac atggggcgga aagcggacgc cgtgcgcgaa
      961 gctgagaagg cgactagcga gactccgcgg gccaaggatt actatcaggg tacgtactat
     1021 ctgcatcagc ttgcgcgggt atacgtgttg gtcggggaac ccgacaaagc catcgcgctg
     1081 ctcgaacagg tgctggcgag tccatattac ctgtctcctg cctggctgcg aatcgacccc
     1141 acgctcgaac cgctccggaa gaatccccgc tttcagaagc tcgtagaagg gactgcgtga
     1201 gcgccgacct ccgcgctgcg ctcgagaccg gccttgctgg tcgttatgcg atcgagcgcg
     1261 agctcgggcg cggcggcatg gcaatggtgt acctcgcgcg cgatctgcga cacgaccggc
     1321 ctgttgccct aaaagtcctc catcccgaac tcggggccgc gctcggcggg gaccggttcc
     1381 agcgcgagat acgacttgca gcccggcttc agcatccgca catccttacc gtccacgact
     1441 cgggtgaaat cgcctccgcc agcggcccac cgctgctctg gttcactatg ccgttcgtgg
     1501 acggcgagac gctccgtgcc cgcttgcagc gcgagcgaca actctccgtc gaggaggccc
     1561 tgaccatcgc gcgggaggcg gcggatgcac tcgactacgc ccaccagcaa ggcatcgttc
     1621 accgcgacat caagccggag aacattctcc tcacggcgcg ccacgcgctg gtagcggact
     1681 tcggggttgc tcgcgcgctg gccggggcgg agcaggggct gaccgagacc ggcatgacgc
     1741 tgggcacgcc ggcatacatg agccccgagc aggccagcgg ggagcggtcg atcgatggcc
     1801 gtaccgacat ctactcgctt ggctccgtgc tctacgaaat gcttgcggga cagccaccgt
     1861 tttccggcgc cagcgctcag gcgatcatcg ctaaacgttt caccgagacg gcaccgccgg
     1921 tccggcgctc gagagagagt gtgtcgcccg agatcgacgc cgcgctcact cgcgccctgg
     1981 cgcggctgcc cgctgatcgg ttcgcgaccg ccggcgaatt cgccaaggcc ctcaccgtgc
     2041 aggcgacgtc gcctgccgtc ccgaccgcgc tcgctcccga ccggccgtca gcgacgacgc
     2101 cgccagtggg agccgtgctt tcgtcggaaa ggcaagccgc cgcggtgtcg accatggcag
     2161 gccatcaccg attccgcgtc cccgcttggc tcgccttcgt cctcggcctt ttggtcacgg
     2221 ccagtatggg catgctgatc tggcagcgga gtcatcgcgc cggagagccc gccgtagccg
     2281 ggcccaagcg actggccgtg gttccgttcg agaatctggg agctccggag gacgagtatt
     2341 tcgcggacgg gatgacggac gaagtccgcg gcaagctggc ctcagtctcc gggctccagg
     2401 tcatcgccaa ccggagtgtg gccgaataca agcggagcac caagagctac cagcagatcg
     2461 gccaggagct cggggtgcag tatctgctga tcggcaaagt tcgctgggaa aagcaggcgg
     2521 gtgcacagag ccgggtccgg gtaagccccg agctgatcga cgtctccagc tcgagcacta
     2581 aatggcagca gccattcgat gccgcgctca ccgacgtgtt ccaggtgcag ggcgagatcg
     2641 ctggtaaggt ggcgcgggcg ctcgacgtgg ccctcggtga gcatgaacgg gagcggatcg
     2701 aggtgcggcc cacccagaac ctcgctgcct acgatgcatt tctccgaggc gaagaggcgt
     2761 cggaccggat gagcaccaca gatcccccgg cgctctcgcg ggcggcatcc gcgtatgagc
     2821 aagctgtcgc ccttgactcc acatttgccc tggcctgggc gcgcctctcg caggcgcggt
     2881 cgaacattta cgggaacggg gttataactg cggtcggtat cgagcaggct cggcgggcag
     2941 ccgagcgagc cctcgccttg gcgcccgatc ggccggaag
//
LOCUS       DASRJQ010000581         2456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3191696, whole genome shotgun sequence.
ACCESSION   DASRJQ010000581 DASRJQ010000000
VERSION     DASRJQ010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3191696"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VFB89_13200"
     CDS             complement(<1..394)
                     /locus_tag="VFB89_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HZM28298.1"
                     /translation="MKGAFLGLGAIGEPMAARLSSRVDLAVWNRTRMRADTFARTNKA
                     RVAATPREAATGTQVVVTCLPTSAEVERLLDGPDGLEAGLGPGTLLLDCTSGDPATSR
                     RIAGRLAGKQVGFADCPVSGGTAGAQSGT"
     gene            complement(391..915)
                     /locus_tag="VFB89_13205"
     CDS             complement(391..915)
                     /locus_tag="VFB89_13205"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HZM28299.1"
                     /translation="MLSAPMQDAFNEQIKNEFYSAYIYLSMAAFFDDVNLPGMASWMR
                     VQAREEAIHAIKIFDHLLDRGARVKLRAVPQPPVEFASPLDVFEQAYQHEQTVTKAIH
                     DLYAKAIQEKDFASSVFLDWFVKEQVEEEKAALVIVEHIKMVAEDRPGLLVLDRELAQ
                     RKGGTETTATQATT"
     gene            972..2246
                     /gene="dinB"
                     /locus_tag="VFB89_13210"
     CDS             972..2246
                     /gene="dinB"
                     /locus_tag="VFB89_13210"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HZM28300.1"
                     /translation="MSTRIMHVDLDAFFVEVCRQRHPELRDLELLVVGGRRDQRGVVQ
                     SASYGARVYGIRAGMPIAEAVRRCPQATFFQGSFNHYRDASRAVRRVLSRFSPTVVMA
                     SLDEGYLDFTGTDRLHPVSLLPVAEGLRDAIRTDTGLACSIGIGSNRMIAKLASDTAK
                     PRGLMEVRAGWEPGFLAGLPLRALPGIGPKTAARWATLGLTDVAQVQAMTLLELTDLL
                     GGDAKLLKLRAEGYGGTALHAERLPRSVSRETTLSRDVRDPEWLDRTLALLTARVASQ
                     LREEGLVARTVTLKLRHDDFYTVTRRHTLREPTDLDAELLGAARTLFRPAFADVRRRN
                     RAVRLIGIAATNLGTAASPDLFETEQRSRQRRLTQAVDEVRERFGFDAVGQGNLMKQQ
                     KRKRFSSESPRSQIGAPPPIPPIAPRHRDRGT"
     gene            complement(2164..>2456)
                     /locus_tag="VFB89_13215"
     CDS             complement(2164..>2456)
                     /locus_tag="VFB89_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28301.1"
                     /translation="GPRLDTGDYFAPLITNATESNLRIVVNAGLDGALDCGCAVRPGA
                     HHVYLGYYRLYRNSTVQATDPQGRHATFRDLGAEAQSGGWVVGLRFASGDFH"
BASE COUNT          415 a          801 c          795 g          445 t
ORIGIN      
        1 gcgtcccgct ttgagccccc gccgtgccgc cactcaccgg gcagtcggcg aaaccgactt
       61 gcttcccggc caaccgaccc gcaatccgcc gtgaagtcgc tgggtcgccc gaggtgcagt
      121 ccaacagcag cgtgccgggg ccgaggcccg cctcgagacc gtccggcccg tcgagcagtc
      181 gctcgacctc cgccgaggtg ggcaggcagg tgacgacgac ctgggtgccg gtcgccgcct
      241 cacgcggtgt ggcggcgaca cgagccttgt tcgtccgtgc gaaggtgtcg gcccgcatcc
      301 gggttcggtt ccacacggcg agatcgaccc gactggagag tcgggcagcc atgggctcac
      361 cgattgcgcc cagtccgaga aaggctccct tcacgtcgtt gcttgcgtcg cggtggtttc
      421 ggtgccgccc tttcgctgag ccagctcgcg atcgagcaca aggagccccg gccgatcctc
      481 ggcgaccatc ttgatgtgct ccacgatgac cagggcggcc ttttcctcct caacctgttc
      541 cttcacgaac cagtcgagga agacggagct tgcgaagtcc ttctcctgga ttgccttggc
      601 gtacagatcg tgaatggctt tcgtgaccgt ctgctcgtgc tggtaggcct gctcgaacac
      661 gtcgagcgga gacgcgaact ccaccggcgg ctggggaacc gcacggagct ttacccgcgc
      721 gccgcgatcg agcagatgat cgaatatctt gatcgcgtgt attgcctctt ctcgcgcctg
      781 cacccgcatc cagctggcca tgccgggaag gttgacgtca tcgaaaaagg cggccatcga
      841 cagatagatg tatgccgagt agaactcgtt cttgatctgt tcgttgaatg cgtcttgcat
      901 cggggcgctc agcatgacgg gcactcctct cgcctggggc atggagccgc ggtagagagg
      961 cgcaagatac gatgagcacg cgaatcatgc acgtggacct cgacgccttc ttcgtcgagg
     1021 tctgccgaca gcggcacccg gagctccgcg acctagagtt actcgtggtg ggtggccgtc
     1081 gcgatcagcg cggcgtggtg cagtccgctt cgtacggtgc gcgggtctac gggattcggg
     1141 ccggaatgcc gatcgcggaa gcggttcgcc gctgtcccca ggcgacgttc ttccaaggct
     1201 ccttcaatca ctatcgagat gcgtctcgcg cagtccgacg cgttctcagc cgattttccc
     1261 ccacagtcgt catggcctcg ctggatgaag gctatctcga cttcacgggg accgaccggc
     1321 tgcatccggt ttcactgttg ccggtagccg aggggttgcg ggacgcgatt cgcaccgata
     1381 ccgggctcgc ctgcagtatc ggcatcgggt ccaaccgaat gatcgcgaag ctcgcctccg
     1441 atacagccaa gccgcgggga ttgatggagg tccgagcggg atgggagccc gggtttctcg
     1501 ccggactccc gctccgcgcg ctgccgggca tcgggccgaa aaccgcggcg cgatgggcga
     1561 cgctgggtct caccgacgtg gcccaggtgc aggcaatgac actgttggag ctgaccgatc
     1621 tgctgggagg tgatgcaaag ctgctcaagc tccgggcgga gggctacggc gggacggcgc
     1681 ttcatgccga gcggctgccg agatcagtaa gcagggagac gaccctgagc cgggacgtgc
     1741 gcgatccgga atggctcgac cgcacccttg cgctgctcac tgcgcgagtg gcttcacagc
     1801 tacgggagga ggggctcgtc gccaggacgg tgacgctcaa actccggcat gatgacttct
     1861 acactgtgac tcgccgccac accctgcgag agccgaccga tctcgatgcc gagctgcttg
     1921 gcgcagcacg gacgctcttc cggccggcct tcgccgacgt tcgccggcgg aaccgcgccg
     1981 tgcgtctgat cggcatcgcc gcgaccaacc tgggcactgc ggcctccccc gatctcttcg
     2041 aaacggagca gcggtcacgc cagcgccgtc tcacgcaagc agtcgacgaa gttcgggaac
     2101 gatttggatt cgatgcggtg ggacagggga atctgatgaa gcaacaaaag cgtaaacgct
     2161 tttctagtga aagtccccgc tcgcaaatcg gagccccacc acccatcccc ccgattgcgc
     2221 ctcggcaccg agatcgcgga acgtagcgtg tcgtccctgc gggtccgttg cctgaaccgt
     2281 gctgttccgg tagagtcggt agtaaccgag atagacgtga tgcgcgccgg gtcgcactgc
     2341 acagccgcag tcgagtgccc cgtccagtcc cgcattcacg acgatccgca ggttgctctc
     2401 ggtcgcattg gtgatgagcg gcgcgaaata gtccccggtg tccagcctgg gccccg
//
LOCUS       DASRJQ010000582         3223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_941972, whole genome shotgun sequence.
ACCESSION   DASRJQ010000582 DASRJQ010000000
VERSION     DASRJQ010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941972"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..214
                     /locus_tag="VFB89_13220"
     tRNA            142..214
                     /locus_tag="VFB89_13220"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:175..177,aa:Gly,seq:ccc)
     gene            1155..1292
                     /locus_tag="VFB89_13225"
     CDS             1155..1292
                     /locus_tag="VFB89_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28302.1"
                     /translation="MSIRIEWWRDGRRIGWRSYRVTSYLFYDQYSRGPFGPYAYCTRW
                     L"
     gene            complement(1582..1704)
                     /locus_tag="VFB89_13230"
     CDS             complement(1582..1704)
                     /locus_tag="VFB89_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28303.1"
                     /translation="MHDFDGDVSYDALGSLEFAQPAAGREADDGSRTRDLRLGN"
     gene            1723..2145
                     /locus_tag="VFB89_13235"
     CDS             1723..2145
                     /locus_tag="VFB89_13235"
                     /inference="COORDINATES: protein motif:HMM:NF019473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZM28304.1"
                     /translation="MSQEDVEVVRRALAALGRRDVEAYLEVASPQIELITFFSLQGPI
                     TGHQGIRDFFRELWSFSEASEFQVEEVRAVGERVLAFFVITATGRDGIQTSARVAGVY
                     DLEHGKIRRAQIFADRAEALEAVGVRDSPLDGDGSGSG"
     gene            complement(2158..2529)
                     /locus_tag="VFB89_13240"
     CDS             complement(2158..2529)
                     /locus_tag="VFB89_13240"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZM28305.1"
                     /translation="MSSENVEIVRTFVLGEFPDVLEFVDPDIVWNPVEESPTRGHEAV
                     RASTERWKREWDDYELTLEDFEDMGDRVLATVRFRARGRGSGIAVGARLYDLFTLRDG
                     KIVRMDQFTERAEALVAAGRG"
     gene            complement(2594..2764)
                     /locus_tag="VFB89_13245"
     CDS             complement(2594..2764)
                     /locus_tag="VFB89_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28306.1"
                     /translation="MIEVCATWKARTSWLVTGFSPPRRQMHLSTDIPLTSSFEVIMEV
                     EPEGTGEAAAAS"
     gene            2791..>3223
                     /locus_tag="VFB89_13250"
     CDS             2791..>3223
                     /locus_tag="VFB89_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28307.1"
                     /translation="MTGSKRTATRVAVAGALPPPNWPRSGFNPDHDHKVSDEIRFVQL
                     TPPGSACSIALGTGGGGHAARLAPGPAARGIGHTAARDELAGRGADVTDVAGLAAAQI
                     DGAAEGSGPGNGRSGFGLGSGSGVGVGGSGSGMGSGSGVSIS"
BASE COUNT          545 a         1035 c         1065 g          578 t
ORIGIN      
        1 ggtgaatcac aaccgcggtg gccacgatga gcacgacgag gaggaacagg ccggcgaaaa
       61 atgcgagtcg cttcatactc cgtcgatccc cggcgcgcgc cagtccgcaa cgtggaccgg
      121 aggctagaat cggactcgca ggcggacgta gctcagttgg tagagcgtcg gcttcccaag
      181 ccgaaggtcg cgggttcgag acccgtcgtc cgctcctcac gatctccctg caaaccgcca
      241 ctttctgagt ccgttccccc atgggagaat ggctgagcat caggagcggg tacacactac
      301 gggtaccttt ctcgctctcc ctcgtcctga gcgctgaagc ctgctcgtat gcacgcatgg
      361 tgaggctcgg atccttcaga tttgcagggc tttcgttgcg tggtgagggc atggtgaccg
      421 ccattggcgg cctcgcgctc gatcgtgggg ttagcgagaa tcagcgagag cccttgcacg
      481 aattcgcctg tgagttgagt aggacttcgt acggccctaa ggaacggggg gacatggcag
      541 tcgtatgctc gggtttggct tgcactgtgg tcgggatagg cacaccatgg aggcgcttgg
      601 tgggcaccgg tcaggcgagg aatgttggcg gagggctagt gatttgtcag actcgcggaa
      661 acgctgtagt tgagctagtc tcgttgggtt aggcgcagac aggggcgttg gagcccgttc
      721 cttacctgcc gactcggaag ggaaggaact ccatgaagac gatcaagctg ggcgtggcgc
      781 tttgcgctgc gctcgtagtc cccacctcat tcgtccctgt tatctcagag gacgctcatg
      841 ccgcacttgt cgtaggtgga gagggttggc gtggaagcag cagcgtcccc ggggcactct
      901 gcaagtacgg cagcactggc ctctacggtt tcttgaggtc caatatcgcc tcgccctctg
      961 tcactggtgc taacaccacc agccgtcgtg gcgaccgcac ctccgcccgc tacgcagtct
     1021 ggctcaacaa tccgcacaca ggtgccaact acgaggtttc aaattggagc tcctggctcc
     1081 tcgtacaaga gggagtgtgg agaacgtggg gcggtttcac tcagttcacc cggaactgga
     1141 atgaggacaa ccgtatgtcg atacggattg agtggtggcg cgacggccga cgtatcggct
     1201 ggcgttcgta ccgagtgacc tcgtacctct tctacgacca atacagcagg ggaccctttg
     1261 gcccctacgc gtactgcact cgctggctct gaggtggcgc tcgctgccat gcagacgtct
     1321 ggcacattgc gggcgctagc ggcagtgtct tcgacgacta ccagacccac ctggggcggc
     1381 gtttctcgaa gctcggcgac acctttgacg gggccctggc ggctgtcaac gcacgtcttc
     1441 aaatattggg ttgacccgcg cgggtacggt gcccggttga ccaggcacca gacgagggac
     1501 cgaagcgcgg aaaactcagg cgactagcca caacgagacc gcgtcctaga gcgaaacggc
     1561 gtcttgcgcg ccttgcgtgg atcaattccc aagccgaagg tcgcgggttc gagacccgtc
     1621 gtccgcttca cgccccgccg ccggctgcgc aaactccaag ctgccgagcg cgtcgtagct
     1681 cacgtcgccg tcgaagtcat gcactgggat actcgcccgg caatgtcgca ggaggacgta
     1741 gaggtggtac ggcgggcact cgcggcgctc ggtcgtcgcg atgtcgaggc gtacttggaa
     1801 gtggcctcgc cgcagatcga actaattacc ttcttctcac tccaagggcc gatcaccggg
     1861 caccaaggca tacgcgactt ctttcgcgag ctgtggagct tctcggaggc gagcgaattc
     1921 caagtcgagg aggtcagagc cgtaggggag cgggttctgg ccttcttcgt catcaccgcc
     1981 actggccgag acggcatcca gacctccgcg cgggtcgccg gcgtctacga cctcgaacac
     2041 gggaagatcc gccgcgccca aatcttcgcc gaccgcgccg aagccctcga agccgttggg
     2101 gtgcgggata gcccgctcga cggcgacggc tccggctccg gctgacagtc cgctccgcta
     2161 cccccgcccc gccgccacca gcgcctcggc ccgctccgtg aactgatcca ttcgcacgat
     2221 cttgccgtcg cggagcgtga acaggtcgta gaggcgggcg ccgaccgcga ttccgcttcc
     2281 tcgcccgcgg gctcggaagc ggacggttgc cagcacgcgg tcgcccatgt cctcgaagtc
     2341 ctcgagggtg agctcgtagt cgtcccactc gcgcttccag cgctcggtgc tcgccctgac
     2401 ggcctcgtgg ccccgtgtcg gggattcctc gacggggttc cagacgatgt ccgggtccac
     2461 gaactcgagc acgtcgggaa actcgccgag cacgaacgtg cggacgatct ccacgttctc
     2521 gctcgacatc agcgtgcggc caccgaggcg gagttctcga gcgagcggcg gttgtcacgg
     2581 gcaaccgagc tcttcatgag gcggccgcgg cctcccccgt gccctcgggc tcgacctcca
     2641 tgatcacctc gaacgagctg gtgagcggga tgtcggtgct caggtgcatc tgccgccgcg
     2701 gcgggctgaa cccggtcacc agccagctgg tgcgggcttt ccaggtcgcg cagacctcga
     2761 tcacggcggt accttctcac gcgagccatc gtgaccgggt ccaagaggac agcgacccgt
     2821 gtcgccgtcg cgggcgccct gccgccgccg aactggccga ggagcggctt caaccccgac
     2881 cacgaccaca aggtgagcga cgagatccgc ttcgtgcagc tcacgccccc gggctcggcc
     2941 tgctcgatcg cactcgggac cgggggtggt ggacacgccg cccggctcgc tccagggcct
     3001 gcagctcgtg gtatcggaca tacggccgcc cgcgacgagc tcgccgggcg tggcgcggac
     3061 gtgacagacg tcgcgggact agctgccgct cagatcgacg gcgccgccga aggatccgga
     3121 cccggtaacg ggaggtccgg gttcgggctt gggtccggct cgggtgtcgg agtcggcggc
     3181 tctggatcgg ggatggggtc gggcagcggg gtctccatca gtc
//
LOCUS       DASRJQ010000583         2080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_942289, whole genome shotgun sequence.
ACCESSION   DASRJQ010000583 DASRJQ010000000
VERSION     DASRJQ010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942289"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2080)
                     /locus_tag="VFB89_13255"
     CDS             complement(<1..>2080)
                     /locus_tag="VFB89_13255"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZM28308.1"
                     /translation="PMQLECFLRLAIGIAAAVGKVHEMGLIHKDIKPANILVELASSG
                     VWLTGFGIASRLPRERRAPAPPEVIAGTLAYMAPEQTGRMNRSVDSRSDLYALGITLY
                     EMLVGTLPFTAADPIEWVHCHIARQPMPLDERVAGVPEPLSAIVMKLLAKTGEERYQT
                     AAGVEADLRRCLAEWESRGRIEPFSLGGCDVSDRLLIPEKLYGREREIETLLASFDRV
                     VANGTLELVLVSGYSGIGKSSVVNELHKVMVPPRGLFASGKFDQYKRDIPYATLAQAF
                     QSLVCSLLSRSEAELDQWRDSLSEALGPNGQIIVDLVSELELLIGKQPPVVDLPPQDA
                     LNRFQMVFRNFLGVFARKEHPLALFLDDLQWLDRATLDLLEHLITHSEVRHLLLVGAY
                     RDNEVSPSHPLLRTLDAIRKAGARIQEIVLAPLGLDDVGQLVADAMHCELEHAQPVAQ
                     LVQKKTGGNPFFVIQFFTALAEERLLAFDPVTRAWQWHMDRIRAKSYTDNVVDLMAGR
                     LKRFSPTTQEALKQLACLGNVAPTAMLALVYGTTEQAMHVALWDAVHAGLVLHRDGAY
                     KFLHDRIQQGAYSLIPEEQRGEVHLRIGRVLRSSLATEEFAEHLFDVANQLNRGAALL
                     VDRDEKAQVATINLRAGRKAKASAAFASARAYFAGGMALLDERDWGSRYELMFSLWLE
                     RAECEFLSGDF"
BASE COUNT          382 a          677 c          652 g          369 t
ORIGIN      
        1 gaagtcaccg ctcaaaaact cacactccgc gcgttcgagc cataggctga acatcaattc
       61 gtaccggctg ccccagtctc tttcgtctag cagggccatg ccgcctgcaa aatacgcgcg
      121 cgccgatgca aaggccgccg acgctttggc ctttcgcccc gcacgcaggt tgatcgtggc
      181 cacctgcgct ttctcgtcgc ggtcgaccag cagcgcggcg ccccgattga gctggttcgc
      241 gacatcgaac agatgctcgg cgaactcctc cgtcgccagg ctcgagcgca gcacgcggcc
      301 gatgcgcagg tgcacttcgc cacgctgctc ttctggaatc agtgaatacg ccccttgctg
      361 gatccggtcg tgcaggaatt tgtaggcgcc atcccgatgg aggacgagcc cagcgtggac
      421 ggcgtcccag agtgccacgt gcatcgcctg ctctgttgtt ccgtaaacca gggcaagcat
      481 cgcggtcggg gcgacattgc cgaggcaggc aagctgtttc agggcttcct gggtggtagg
      541 agaaaaccgc ttcagcctcc cggccatgag atccaccacg ttgtcggtgt agctcttggc
      601 acggatgcga tccatgtgcc attgccaggc ccgcgtgact gggtcgaacg cgagcagccg
      661 ctcttcggcc agcgccgtga agaactggat cacgaagaac ggatttccgc cggtcttctt
      721 ctgcaccagt tgcgctacgg gttgcgcgtg ctccagttcg caatgcatgg catcggcgac
      781 gagctggccg acatcgtcga gtccgagagg cgccagcacg atctcctgga tccgcgctcc
      841 ggctttgcgg atcgcgtcta gcgtccgcag aagcgggtga gagggactga cctcgttgtc
      901 ccggtaggcg ccgaccagca gcaggtgccg cacttctgag tgcgtgatca gatgctcgag
      961 caggtcgagc gttgctctat ccagccattg caaatcatcg aggaacagcg cgagagggtg
     1021 ctccttgcgc gcgaacaccc caaggaaatt ccggaatacc atctggaacc ggttcagcgc
     1081 gtcctgcggc ggcagatcca cgaccggtgg ctgtttccca atcaggagct ccagctcgga
     1141 aacgaggtcg acgatgatct gaccattcgg gcccagcgcc tcgctgaggg aatcccgcca
     1201 ctggtcaagc tcggcctcgc tccggctcaa cagcgagcag acaaggctct ggaaggcttg
     1261 ggcaagagtt gcgtacggga tgtcgcgttt gtactggtcg aacttgccgg acgcgaacag
     1321 tccacgcggc ggcaccatga ccttgtgcag ctcgttcacc acggaggact tgccgatacc
     1381 cgaatatccg gacaccagca cgagttccag cgtgccgttc gccacgaccc ggtcgaagga
     1441 agcgagcaga gtctcgatct cgcgctcccg gccgtagagc ttctccggga ttagcagccg
     1501 gtccgatacg tcgcaaccgc cgagcgagaa gggctcgatc cggccacgcg actcccactc
     1561 cgccaggcag cgccgaagat cggcctcgac accggcggca gtctggtacc gctcctcgcc
     1621 ggtcttggcg agaagcttca tgacgattgc cgagagcggc tcggggacgc ccgcgacccg
     1681 ctcatccagc ggcatcggtt gccgcgcgat atggcagtgc acccactcaa tcggatcggc
     1741 agccgtgaag ggcagtgtcc ccaccaacat ctcgtagagg gtgataccca gggcgtagag
     1801 atcgctgcgg gagtccaccg agcggttcat ccggccagtc tgctccgggg ccatgtaggc
     1861 gagcgtgccc gcgatcacct ctggcggggc gggggcccgg cgctcgcgcg gcagacgcga
     1921 agcgatgccg aaccccgtca gccacacgcc gctgcttgcc aattccacca ggatatttgc
     1981 cggcttgatg tccttgtgga tgaggcccat ttcgtggacc ttacccacgg cggcggcgat
     2041 gccgatggcg agccgtaaga agcattccag ctgcatgggc
//
LOCUS       DASRJQ010000584         9012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3756830, whole genome shotgun sequence.
ACCESSION   DASRJQ010000584 DASRJQ010000000
VERSION     DASRJQ010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 9012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9012
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3756830"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            405..1688
                     /locus_tag="VFB89_13260"
     CDS             405..1688
                     /locus_tag="VFB89_13260"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HZM28309.1"
                     /translation="MTMLTVGFAGTAGNVQPAAAQQDTSRAEAQQDTAKAGPKLSADK
                     EGFWLRSADGKFALHLGGYIQADSRTFLDDPANRLTNTFLLRRARALIEGTVFKFFDF
                     RLLPDWGGGTAVIQDAYVAAKLQPYLKLQAGKFKGPVGLERLQSSTDLLFIERAFPTA
                     VAPNRDLGFALTGDIADATVQYSVGIFDGVVDGASLDTDLGDDKDFEGRIYLTPFKRS
                     SIVFLNGLSVGVGGTIGNEHGTQAAPALASYKSPGQNTFFSYRSDAAAAAAVADGQRW
                     RISPQAYWSMGPVGLMGEWIRSSQEVSRTPATATIGVTAWQAAGSFALTGENASYRGL
                     SPRKPVTAKGYGAFEVVGRYSELTVDDDAFPTFADPARSARKAKEWAVGLTWSAERRI
                     KIAVNYGQTSFDGGAAGGADRDTEKALLTRFQVAF"
     gene            1730..2767
                     /locus_tag="VFB89_13265"
     CDS             1730..2767
                     /locus_tag="VFB89_13265"
                     /inference="COORDINATES: protein motif:HMM:NF008106.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HZM28310.1"
                     /translation="MPRRATILAIGLMLPLTVLATGCGGSQAESAPKSVTLLNVSYDP
                     TRELYQEVNTAFAAFWKSKTGQSVTVQQSHGGSGKQARAVIDGLPADIVTLALAYDID
                     AIAERNVIAKDWQKRLPNNSAPYTSTIVLLVRKGNPKHVRDWEDLVRPGISVITPNPK
                     TSGGARWNYLAAWGYALRRWGGDEAKAREFVAKLYRNVPVLDSGARGSTTTFVERGIG
                     DVLIAWENEAILAAKELGQDKFEIVMPTVSILAEPPVAVVDKVVKRHGTEAVAVGYLQ
                     FLYSDQGQEIAAKHFYRPQNSAIAQKYSAQFPKINLFTIDEVFGGWAKAQATHFADGG
                     VFDQIYQPGKA"
     gene            2764..3594
                     /gene="cysT"
                     /locus_tag="VFB89_13270"
     CDS             2764..3594
                     /gene="cysT"
                     /locus_tag="VFB89_13270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HZM28311.1"
                     /translation="MSVRRQKSALPGFGLALGFTLTYLGLVVLLPLSTIFVKSSGLGW
                     HAFWEAVASPRVVASYRLSFLASLIAAAVNSVFGVLVAWVLVRYKFPGRRLMDALVDL
                     PFALPTAVAGIALTTLYAPGGWMGSRLAPLGIQVAYAPLGVMLALTFIGLPFVVRTVQ
                     PVLQDLDADIEEAAASLGATRGQIFWKVLLPMLGPAILTGFALAFARAIGEYGSVVFI
                     SGNMPMKTEIAPLLIMTKLEQFDYAGATAIAVVMLVASFALLLAINVLQWLGRRQAAG
                     "
     gene            3602..4468
                     /gene="cysW"
                     /locus_tag="VFB89_13275"
     CDS             3602..4468
                     /gene="cysW"
                     /locus_tag="VFB89_13275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HZM28312.1"
                     /translation="MTTVAGTAHPGIAPRPMTAATTESPLVRWSLTAIALAFMGLFLV
                     LPLASVFAEALAKGWAAYGRELVTPDALAAIRLTLLAAGIAVPLNLVFGIAAAWAIAK
                     FSFPGKNLLITFIDLPFAVSPVISGMIYVLLFGTQGWLGPWLDEHNITIIFAVPGIVL
                     ATIFVTFPFVARELIPLMEAQGTEDEEAAVVLGATGWQTFWRVTLPNIRWGVLYGVIL
                     CNARAMGEFGAVSVVSGHIRGVTNTMPLHVEILYNEYNFVGAFAVASLLALLALVTLA
                     AKTLVEWRLQRE"
     gene            4505..5362
                     /locus_tag="VFB89_13280"
     CDS             4505..5362
                     /locus_tag="VFB89_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HZM28313.1"
                     /translation="MRWTPGGRSQDLEDRRGEGGSGGGFIPGGGRGLGLGGLVILLIL
                     SLIFKRDFLGLLGGDGVQGGAPTGEVATSPEEEKLADFVSFVLDDAQGTWERLFAARG
                     SSYRHAKLVLFRDAIRSACGFAESATGPFYCPGDEKVYIDLGFYDELRRRFGAPGDFA
                     QAYVLAHEIGHHVQRLLGTEEQVRQAQQRRPDLGNELSVRLELQADCYAGVWANSTAH
                     RDILEQGDVDEGLNAAAAVGDDRIQRMGSGAVHPEAFTHGTSQQRSQWFRTGFKSGQI
                     DVCDTFRDR"
     gene            5362..5772
                     /locus_tag="VFB89_13285"
     CDS             5362..5772
                     /locus_tag="VFB89_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HZM28314.1"
                     /translation="MAGYSSKSLADKLGIKPGTIVTALAAPPAYIDLLKPVPADVTFV
                     NRLGKQARFIHCFVTSRSELEKEFPTLARSLADDGMAWISWPKKSANTGTDLTEDVVR
                     EIGLRAGLVDVKVCAVDELWSGLKFVRRLANRRP"
     gene            5792..6148
                     /locus_tag="VFB89_13290"
     CDS             5792..6148
                     /locus_tag="VFB89_13290"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HZM28315.1"
                     /translation="MASSSYSRIYAVVRRIPRGKVATYGQIAELAGLPGHARQVGYAM
                     HALPSTTSLPWHRVINAKGEVSRRRRPGEELSQRMRLEEEGVGFDLRGRVALKRVRWR
                     PKTRGRQSQARRRKSG"
     gene            6145..6729
                     /locus_tag="VFB89_13295"
     CDS             6145..6729
                     /locus_tag="VFB89_13295"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZM28316.1"
                     /translation="MSELPTIRTERLVLNRFDPEDIPELEKLAGAPEIADTTLSIPHP
                     YQRSDAERFLTLQTKGAEEGKELIFALHRQPDGRLVGCASLRDIDAENLQAELGYWIG
                     VPYWNQGFATEAARALVHYGLEQLGLNRIYAHHMARNPPSGRVLERVGMQCEGVLRQR
                     VRKWGRFEDVVLYAILRQELVGSPQGASGSIDIA"
     gene            6811..7812
                     /locus_tag="VFB89_13300"
     CDS             6811..7812
                     /locus_tag="VFB89_13300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZM28317.1"
                     /translation="MGRKAAGPRSSEGMYRSIVEQSQGLICVHDLTGVLRYTNPASAA
                     ELGVTPADLIGRNGRTLLAPWLQPLFDEYLERIRREKIDHGVLRLTRRDGEERLWAYR
                     NVLHDDNGNEPYVIGHAVDITDYWRTKQKLRESEQRSGAVLAALEEGIVFCDASGSVK
                     ESNASAERILGRSPEHLFGKGAANSPWRVILPDGSPSPLDQRPEAVTLRTGEPCLRVV
                     LGLEQGNGTVQWLEINSQPLIGRKSKAPYGAVISFTDVTERRLQESARERELEAALAG
                     LKVLRGLLPICASCKKIRGDDGDWVQLETYITRHSEADFTHGLCPTCLAELDSALGP"
     gene            complement(7809..>9012)
                     /locus_tag="VFB89_13305"
     CDS             complement(7809..>9012)
                     /locus_tag="VFB89_13305"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HZM28318.1"
                     /translation="ARIRPGVPFDPAAATAAYIATIPAKDRARSDAYFEGGYWIQLWG
                     FLISVAVLLLFLHLGWSRRLRDWADRVGRWRWARTFLYYGAFTIIGGVVTFPWTIYTG
                     FVREHAYGLATQSFGGWLGDQAKALAVNVILGGIAVAVLYAVARKLPRTWPAWGAAAC
                     VVFLIIGALIGPVFIAPLFNKYTPLTDARIRDPILRLARSNGIETDKVYVVDESRQSK
                     RVSANVSGILGTQRISLNDNLLRLSTLPEVESVMGHEMGHYVLHHVYMYILFFTLVIV
                     AGFAVLRSGFLWAMARWGDRWGLRGIDDPAGFPLAILILSIYFFVLTPAFNNFIRADE
                     AAADIFGLNAVREPDASALVDLKLVEYRKVDPGPIEEFLFYDHPSPRSRIYQAMEWKA
                     EQLAPPSR"
BASE COUNT         1635 a         2784 c         2975 g         1618 t
ORIGIN      
        1 ctactacaat cactacgcat ttaaatcctt caaaaatcaa tagttaccgg tttgatccgt
       61 cgacggcaca tggcctgccc ggaggtctgt cggaccaggg gcaaaacgga cgactatggc
      121 cgcgacctac tcgacaacca atggcgatcg cctcgatccg gcgctcgctg ccgcactcgc
      181 agctgctctc cgcggacttc gcttcggatc catagaaatc gtcgtacacg actcacgagt
      241 cgtgcagctc gaacggcgcg agcgtttgcg gctcgcgagc tccgaatcgg gttcgaacag
      301 cgggcaataa gaatggtggc tcaactgcgt cggccgaccg gactgccgga ggcccttgac
      361 tcgaaagcga gggtttcgtg cgcgtggtcc ggtttggatg ggcaatgaca atgctgactg
      421 ttggattcgc cgggactgct ggaaatgtcc agcccgctgc ggcgcagcag gatacctcgc
      481 gcgccgaggc acaacaggat acagccaagg ccggacccaa gctgagcgcg gacaaggagg
      541 gattctggct tcgctccgcg gatgggaagt tcgcgctgca tctcggagga tatatccagg
      601 ccgacagccg gacgttcctc gatgatcccg ccaaccgtct gaccaacacg ttcctgctgc
      661 gccgggcccg cgcgctcatc gaaggcacgg tattcaagtt cttcgatttt cgcctgctgc
      721 cggactgggg cggcggtacg gcggtcattc aggacgccta tgtggccgcg aagcttcagc
      781 cctacctcaa gctccaggcc ggaaagttca aaggcccggt cggtctcgaa cggctccaat
      841 cgtcgacgga cctcctgttt atcgagcggg ctttcccgac ggcggttgcg cccaaccgtg
      901 acctgggctt tgcgctcacc ggtgatatcg cggacgcgac ggtccaatac agcgtcggga
      961 ttttcgacgg cgtggtggat ggagcgagtc tcgataccga cctgggtgac gacaaggatt
     1021 tcgaaggcag gatctacctc acgccgttca aacggtccag catcgtcttc ctgaacgggc
     1081 tgagtgtggg agttgggggt accatcggaa acgagcacgg cactcaggcg gcacctgcgc
     1141 tcgcgtcata caagagtccc ggccagaaca cgttcttcag ttatcgaagc gacgccgcgg
     1201 ctgctgccgc cgtggccgac ggtcagcgtt ggcggatctc gccgcaggcg tactggtcca
     1261 tggggccggt gggactcatg ggcgagtgga ttcgctcgtc ccaggaggtc agccggacac
     1321 cggcaactgc cacgatcgga gtgacggcgt ggcaggcggc gggctcgttc gcgttgacgg
     1381 gcgagaacgc gagctaccgc gggctctcgc ccaggaaacc ggtcacggcc aagggctacg
     1441 gcgcgttcga agtcgtggga cgatacagcg agctcacggt cgatgatgat gcgtttccca
     1501 ccttcgctga tccggcgcgc tcggcccgca aggcaaagga gtgggcggtg ggtctcacct
     1561 ggtctgccga acgtcggata aaaatcgcgg tgaactatgg ccagaccagt ttcgacggcg
     1621 gcgctgccgg tggtgcggac cgcgacaccg agaaggcact gctcacccgg ttccaggtgg
     1681 cgttctgagc gcaccaggcc ctgacgatat gaatcaagcg agagaaacca tgccacgacg
     1741 cgcaaccata cttgcgatcg gtctgatgtt gccgctgacg gtgcttgcaa cgggctgcgg
     1801 tggctcgcaa gcagagtcgg caccgaagag cgtcacgctg ctcaacgtgt cgtacgatcc
     1861 gacccgcgag ctgtaccagg aggtgaacac tgccttcgcc gccttctgga agagcaagac
     1921 cggacaaagc gtcaccgtgc agcagtcaca cgggggctcc gggaaacagg cccgggccgt
     1981 gatcgacgga ctccccgccg acatcgtgac gctggcgctg gcgtacgata tcgacgcgat
     2041 tgccgagcgg aacgtcatcg ccaaggattg gcagaagcgg ctgccaaaca acagcgctcc
     2101 ctacacctcc acgatcgtgc tgctggtccg caagggaaat cccaagcacg tcagggattg
     2161 ggaagatctg gtgcggcccg gcatctccgt catcacgccg aatcccaaga ccagtggcgg
     2221 cgcccgctgg aactatctcg cggcgtgggg gtacgcgctc cgccgctggg gcggcgacga
     2281 agcgaaggcg cgggagttcg tggccaagct ctatcgcaac gttccggtgc tcgacagcgg
     2341 cgctcgcggc tcgacgacca ccttcgtgga gcggggcatc ggcgacgtgc tgattgcctg
     2401 ggagaatgaa gccatcctcg cggccaagga gctggggcag gacaagttcg agatcgtgat
     2461 gcccaccgtg agcattctcg ccgagccgcc ggtagcggtg gtggacaagg tggtgaagcg
     2521 gcatggcacc gaagcggtgg cggtgggtta tctccagttc ctctattcgg accaggggca
     2581 ggagatcgcg gccaagcatt tctatcgtcc ccagaactcg gccatcgcgc agaagtactc
     2641 ggcccagttc cccaagatca acctgttcac catcgacgag gtgttcggcg gctgggccaa
     2701 ggcgcaggcc acccatttcg ccgacggcgg cgttttcgat cagatctatc aaccgggtaa
     2761 ggcatgagcg tccgacgcca gaagagcgcg ctgcccgggt tcgggctcgc gctgggattt
     2821 accctcacat acctgggcct cgtagtcctg ctcccgctct ccaccatctt cgtgaagagc
     2881 agcggcctgg gatggcatgc cttctgggaa gccgttgcgt ccccgcgggt ggtcgcgtca
     2941 taccggctca gctttctcgc ctcgcttatc gcggcagccg tgaactccgt gttcggtgtg
     3001 ctggtggcct gggtgctggt gcgttacaag tttcccggcc gccgcctcat ggacgcgctg
     3061 gtcgatctgc cgttcgcgct tcccaccgcg gtcgcgggca tcgcgctcac gacgctctac
     3121 gcgcccggcg gctggatggg ctcccggctg gctcccctcg ggattcaggt cgcatacgca
     3181 ccgctcggcg tcatgctggc gctcacgttc atcggactcc ccttcgtagt gcggacggtg
     3241 caaccggtgc tccaggatct ggatgcggat atcgaggaag cggcggccag cctgggcgcc
     3301 acccggggac agattttctg gaaggtgctg ctccccatgc tggggccggc aattctcacc
     3361 ggctttgcgc tggccttcgc gcgggcaata ggcgagtacg gatcggtggt gttcatctcc
     3421 ggcaacatgc cgatgaagac cgagatcgca cccctgctca tcatgaccaa gctggagcag
     3481 ttcgactatg ccggcgccac cgcgatcgcg gtggtaatgc tggtggcgtc tttcgcgttg
     3541 ttgctcgcta tcaacgtgct tcaatggctg ggtcgccggc aggcggcggg gtgagagtct
     3601 gatgactacg gttgccggca ctgcccaccc cggcatagca ccgcgcccca tgacggcggc
     3661 gacgaccgag tcgccgctgg tgcggtggag cctcacggcc atcgccctgg cgttcatggg
     3721 attgttcctg gtgttgccgc tcgcctcggt cttcgccgag gcgctggcga aggggtgggc
     3781 ggcatacgga agggagttgg tcacgccgga cgcgctggcc gccatccggc ttaccttgct
     3841 cgcggcagga atcgcggtgc cgctaaatct cgtcttcggc atcgccgccg cctgggccat
     3901 cgcgaagttt tcgtttcccg gcaagaacct gctgatcacc ttcatcgatc ttccgttcgc
     3961 ggtctcgccg gtgatctccg gcatgatcta cgtactgctg ttcggcacgc aggggtggct
     4021 cggaccctgg ctggacgagc acaatatcac gatcatcttt gccgtgccgg gaatcgtcct
     4081 cgcaacgatc ttcgtgacat tccccttcgt cgcccgcgag cttattccgc tgatggaagc
     4141 gcagggcacc gaagacgaag aggcggccgt ggtgctcggg gccaccgggt ggcagacctt
     4201 ctggcgggtt accctcccca atatccgttg gggcgtcctc tacggcgtga tactctgcaa
     4261 tgcccgcgca atgggtgagt tcggtgccgt atcggtcgtg tcgggacaca tccggggcgt
     4321 caccaacacg atgccgctcc acgtcgagat cctctataac gaatacaact tcgtgggcgc
     4381 atttgcggtg gcctcgctgc tcgcgttgct cgcgttggtg acgctggccg cgaagaccct
     4441 ggtcgagtgg cggcttcagc gagagtagga tagccttcgg agaaccccac gctgggagct
     4501 cgccgtgcga tggacaccgg gtggtcggag ccaggacctc gaagatcgac ggggcgaagg
     4561 cggttccggt ggcgggttca tcccgggcgg tgggcggggc ctcggattgg gcggcctggt
     4621 cattcttctc atccttagtc tcatcttcaa gcgcgatttc ctgggattgc tgggcggcga
     4681 cggcgtgcaa ggaggggcgc ccaccggaga agtcgcgacc tcccccgaag aagaaaagct
     4741 ggcggacttc gtttcgttcg tgctggacga cgcgcagggg acctgggagc gcctgttcgc
     4801 cgctcgcggt agctcatacc gacatgcgaa gctggtcttg tttcgcgacg cgataagatc
     4861 ggcttgtgga ttcgccgagt cggcgaccgg accgttctac tgtccggggg acgagaaggt
     4921 ctacatcgat ctcgggttct acgacgagct gcgccggcgc ttcggggctc cgggcgactt
     4981 tgcccaggcc tacgtgctgg cgcacgagat cggccatcac gtccagcggc tcctgggcac
     5041 cgaggagcag gtgcggcaag cgcagcagcg gcggccggat ctggggaacg agctttcggt
     5101 cagactcgag ctgcaagcgg actgctatgc cggagtgtgg gccaattcga cggcgcaccg
     5161 tgacatcctc gagcagggcg acgtagacga agggctgaac gcggcggccg cggtcggaga
     5221 cgaccggatt cagcgaatgg gcagtggcgc cgtgcatccg gaggcgttta cccacggcac
     5281 ctcgcaacaa cgcagccaat ggttccggac cggattcaag tcgggccaga tcgacgtgtg
     5341 cgacacgttc cgtgatcgct aatggcgggc tactcgtcga aatcccttgc ggataagctc
     5401 gggatcaagc ccggcaccat cgtcaccgcc ctcgctgctc cccccgctta catcgacctg
     5461 ctcaagccgg tgccggccga tgtcaccttc gtcaatcggc tggggaagca ggcccggttc
     5521 atccattgct tcgtgacgag tcggagcgag ctggaaaagg agtttccgac attggctcgc
     5581 tcgctggcgg atgacggtat ggcgtggatc tcgtggccta agaagtcggc gaacaccgga
     5641 accgacctca ccgaagatgt cgtgcgcgag atcggccttc gggccggact ggtcgacgtc
     5701 aaggtatgcg cggtcgatga attgtggtcg ggactcaagt tcgtccgccg gctggcgaac
     5761 cggcggccgt gacctgcttc tggtaggctc ggtggcatcg agctcctaca gccggatcta
     5821 tgccgtggtt cgccgcattc cgcgcgggaa ggttgccacc tatggacaga tcgcggagct
     5881 ggccggtctc ccgggccatg cccgccaggt gggctatgcg atgcatgcgt tgccgtccac
     5941 cacttcgctt ccctggcacc gagtcatcaa cgcaaagggt gaggtgagcc gccggcgacg
     6001 gccgggcgaa gagttgtcgc aacgaatgcg gttggaggaa gagggggtgg ggttcgacct
     6061 tcgtgggcga gtggcattga agcgagtgcg ctggcggccg aaaacccgtg gtcgacagag
     6121 ccaggctcgc aggcggaaat ccggatgagc gagttgccca ccatccggac cgagcggctg
     6181 gtgctgaatc ggttcgaccc ggaggacata cccgagctgg agaagctggc cggggctccc
     6241 gaaatcgccg atacgacgtt gtcgatcccc cacccgtacc aacgatcaga cgcggagcgc
     6301 tttctcacgc ttcagacaaa gggcgcggag gaagggaagg agttgatttt cgcgcttcac
     6361 cgccagcctg acggtcggct ggtcggatgt gcctctctcc gcgacatcga cgccgagaat
     6421 ctgcaggccg agctggggta ctggattggc gtcccctact ggaatcaggg atttgccacc
     6481 gaagcggctc gcgcgctcgt gcactatggc ctcgagcagc tcgggctcaa tcggatttat
     6541 gcccaccaca tggcgcggaa ccccccctca gggcgggtcc tggagcgggt cggcatgcag
     6601 tgcgaaggcg ttctgcgcca gcgagtgcgc aaatggggcc ggttcgagga cgtggtactc
     6661 tacgcgatcc tgcgtcagga gctggtcggg agcccgcagg gggcgtcggg cagcatcgat
     6721 attgcttgac agcgggagac cggctccata tctgtggagc gccgcgcgac attatccccc
     6781 cgacgttcct gcggcgactg aggtgaagca gtgggcagga aggcagcagg accccggtcg
     6841 tccgagggga tgtatcgcag catcgtagag cagagccagg gcttgatctg cgtccacgat
     6901 cttaccggtg ttcttcgcta caccaaccca gcctcagcag cggagctcgg cgtcaccccc
     6961 gcggatctca tcggccgcaa cggccgaact ctgctcgctc cctggctcca accgcttttc
     7021 gatgaatatc tcgaacggat ccggcgtgag aagatcgatc acggcgttct gcggctgacc
     7081 cgtcgcgacg gagaagagcg cctttgggcc taccggaatg tcctgcacga cgacaacggc
     7141 aatgaaccct acgtcatcgg ccacgccgtc gacatcacgg actactggcg aaccaagcag
     7201 aagctccggg aaagcgagca gcgatccggt gccgttctcg cggctctcga agaaggcatc
     7261 gtcttctgcg atgcgagcgg ttcggtgaag gaaagcaatg cgagcgcgga gcggattctg
     7321 ggaaggtcgc cggagcacct tttcggcaag ggagcggcca attccccatg gcgcgtcatc
     7381 ctgcctgatg gctcgccgtc gcccctcgac caacgcccgg aggcggtcac gctgcgtacc
     7441 ggcgagccat gtcttcgcgt tgtgctcggc ctggagcagg gaaacgggac ggttcagtgg
     7501 ctcgagatca attcgcagcc actgatcgga cgcaagagca aagcgccgta cggtgccgtc
     7561 atctcgttca ccgacgtgac tgagcggcgg ctgcaagagt cggcgcggga acgggagctg
     7621 gaggccgcgc tcgccgggct caaagtattg cgcggcctgc tcccgatctg tgcctcgtgc
     7681 aagaaaatcc gtggcgacga tggcgactgg gtccagctcg agacctatat cacacgccac
     7741 tcggaggcag acttcaccca cggtctttgc ccaacctgcc tcgccgagct ggactcggcg
     7801 ctcgggccct agcgactcgg cggagccagt tgctctgcct tccattccat ggcctggtag
     7861 atccggctcc gcggactggg gtgatcgtag aagaggaact cctcgatcgg tcccggatcg
     7921 accttccgat attccaccag cttgagatcg accagggctg atgcgtccgg ctcacgtact
     7981 gcgttgaggc cgaagatatc ggctgcagct tcgtcggcac gaataaagtt gttgaacgcc
     8041 ggggtcagta caaagaagta gatcgacaga atcagaatcg cgagcggaaa ccccgctgga
     8101 tcgtcaatgc cgcgcaggcc ccaccgatcg ccccaccggg ccatggccca gagaaatccg
     8161 gatcgcagga ccgcgaagcc ggctacaatc accaaggtga aaaacaggat gtacatgtag
     8221 acgtgatgga gcacgtagtg tcccatctcg tgtcccatga ccgattcgac ctcaggcaag
     8281 gtggaaagac ggagcaggtt gtcattgagg ctgatccgct gagtccccag gatgccggag
     8341 acgtttgcgc tcacccgttt cgactgacgc gactcgtcca cgacgtacac cttgtcggtc
     8401 tcgatcccgt tgcttcgggc gagccggagt atgggatcgc ggatccgggc gtcggtcagc
     8461 ggggtgtact tgttgaacag gggggcaatg aagaccggcc cgatcaacgc gccgataatc
     8521 aggaacacca cacacgccgc ggcgccccac gcgggccagg tccggggcaa cttccgggca
     8581 acggcgtaga gcaccgccac ggcgatgccc ccgagaatga cattgactgc gagcgccttc
     8641 gcctggtcac ccagccagcc accgaagctc tgggtcgcga gtccgtacgc gtgctctcgg
     8701 acgaagcctg tgtagatggt ccacgggaac gtgaccactc cgcctatgat cgtgaaggcg
     8761 ccgtagtaca ggaacgtcct ggcccaccgc catcgtccga cccgatcggc ccagtcgcgg
     8821 agccgtcgcg accagccgag gtggagaaac agaagcagga ctgcaacgga gatgaggaag
     8881 ccccagagct ggatccagta cccaccctcg aaatacgcgt cggagcgggc gcgatccttg
     8941 gcgggaatgg ttgcgatgta cgccgccgtt gccgctgccg gatcgaaggg tacgccgggc
     9001 cgaatgcgag cc
//
LOCUS       DASRJQ010000585         2954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_91475, whole genome shotgun sequence.
ACCESSION   DASRJQ010000585 DASRJQ010000000
VERSION     DASRJQ010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91475"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1166)
                     /locus_tag="VFB89_13310"
     CDS             complement(105..1166)
                     /locus_tag="VFB89_13310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZM28319.1"
                     /translation="MFMMPLHPPEKPPTVSFEEDIELVVRADELAFTEAWIGQHHSIA
                     WEPIPANDLFIANVLPRTRNIRLGTGVTLVPHHHPVNIAQRLAFLDHLARGRFNCGFG
                     QGGVPTDAELFGLPDPMTQGLMTLEGIDLVLKLWQAEPPFDFKGRFWHVKIEHPDPAI
                     GLGATLKPYQKPHPPVAMTMVKGSSRAAGTAGQRGFIPLSTNLAPIETVAAHWRGYAA
                     GAADAGRTPDRALWRVSRSILVADSSRQAWEHARSGTLARGFDYLTKLLKKAGSLDVM
                     KHAPDVADSDVTWEYCLRHLCIIGDVEECRRQLREVWDRTGGFGTLLMIAHDWDDRAR
                     WLRSAELLAREVVPALPSI"
     gene            complement(1223..2218)
                     /locus_tag="VFB89_13315"
     CDS             complement(1223..2218)
                     /locus_tag="VFB89_13315"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HZM28320.1"
                     /translation="MSLKVALVGLDCPGTPAWVTERLAAERITFAMRECTTPEELAET
                     AGDADVVWIFGGSRIVSQSTLPLLARCGAIIRTGSGTDNVPVEAATRIGIVVANTPEA
                     LNEAVADHAVALLLAAARQIAVQDRAVRRGVWDRAIAWPRFRLQRQTLGLVGFGHVAR
                     AVARKLRGFEMNLVVFDPKLDAEPIASLGARSAPLDEVLGDADFLSIHCPLTAETRHL
                     IGEAELRRMKPDAILVNTSRGPVVDEAALARALKEHWIAAAGLDVLEHEPPHPDNPLL
                     ALENVIITPHIAGYSPGYEEAAWRLSVETVIDLAQGRWPRSYVNPGVRPRMNLRP"
     gene            complement(2285..2500)
                     /locus_tag="VFB89_13320"
     CDS             complement(2285..2500)
                     /locus_tag="VFB89_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28321.1"
                     /translation="MAREPNVTLRIRSAECLGPDRGRRRTDQGGRVLDYLGKPNRKMC
                     SLYLSLLDKCGIHMKEFGDSNERLADV"
BASE COUNT          513 a          950 c          986 g          505 t
ORIGIN      
        1 tcccctgcgt cgccgagagt ttacggcgta ggcgcgcatt agatgctgga gttccgggac
       61 cagcggacta tccattgctc gtttctcccg ggacgccgca cccctcagat ggacggcaaa
      121 gcggggacaa cctctctggc caggagctcg gccgagcgca gccaccgggc gcgatcgtcc
      181 cagtcatgcg cgatcatcag cagggtgccg aaaccgcccg tccggtccca cacctcccgg
      241 agctgccgcc tgcactcctc cacgtcgccg atgatacaga ggtgacggag gcagtactcc
      301 caggtgacgt cactgtcggc gacgtcgggg gcgtgcttca tcacgtcgag gctgcccgcc
      361 ttcttcagga gcttggtcag gtagtcgaag ccgcgggcca gcgtgccgct gcgggcgtgt
      421 tcccaggcct gccggctcga gtcggccacc aggatgctgc gagacacgcg ccacagggcg
      481 cggtccggcg tgcgacccgc gtcggcggcg ccggccgcgt agccgcgcca gtgtgcggcg
      541 accgtttcga tgggcgccag gttcgtgctg agcgggatga atccgcgctg tccggcggta
      601 cccgccgcgc gcgagcttcc cttgaccatg gtcatggcga caggcggatg gggcttttgg
      661 tagggcttga gcgtagcgcc caggccgatc gccgggtcgg gatgctcgat cttcacgtgc
      721 cagaagcgac ccttgaaatc gaacggcggc tccgcctgcc agagcttcaa taccaggtcg
      781 atgccctcga gggtcatcaa gccttgggtc atcggatcgg gcagaccgaa cagctccgcg
      841 tcggtcggca cgcctccctg gccgaacccg cagttgaagc ggccgcgcgc caggtgatcg
      901 aggaacgcca gccgctgggc gatgttgacc ggatggtggt ggggaacgag cgtcacccct
      961 gtgcccagcc ggatgttgcg ggtgcgcggc aggacgttgg cgatgaacag gtcgttggcc
     1021 gggatcggtt cccaggcgat cgagtgatgc tggccgatcc aggcctcggt gaaggcgagc
     1081 tcgtcagctc ggacgaccag ctcgatgtcc tcctcgaagg agaccgtggg cggcttctcc
     1141 ggcggatgca gcggcatcat gaacattcca agcttcacgg gtacgtctcc ttcatgaggg
     1201 cgttgggact caacgccggc cctcagggcc tcaggttcat ccgcggcctc actcccgggt
     1261 ttacgtagga ccgcggccag cgaccctgcg cgaggtcgat caccgtctcg accgagaggc
     1321 gccaagccgc ttcctcatag ccgggcgagt agcccgcgat gtgcggcgtg atgatgacgt
     1381 tctcgagcgc aagaagcggg ttatccggat ggggaggctc atgctcgagc acgtcgagcc
     1441 cggccgcggc gatccagtgt tccttcaggg cgcgggccag cgcagcttcg tcgaccaccg
     1501 ggcctcgcga cgtgttgacc aagatcgcgt cgggtttcat gcgccgcagc tcggcttcgc
     1561 cgatgaggtg tcgcgtctcc gcagtcagtg ggcagtggat cgagagaaaa tcggcgtcgc
     1621 ccagaacctc gtcgagcggg gccgagcgag cgccgaggga cgcgatcggc tcagcatcga
     1681 gcttcgggtc gaagacgacc aggttcatct cgaagccgcg caacttgcgc gccaccgccc
     1741 gggcgacgtg gccgaagccg acgagcccga gcgtctggcg ctgcaggcgg aaccggggcc
     1801 aagcgatggc acgatcccag acgccgcgtc gcaccgcccg atcctggacc gcgatctgac
     1861 gggcagcggc caggagcagg gcgaccgcgt gatcggcgac tgcttcgttc agcgcctcgg
     1921 gcgtgttggc caccacgatg ccaatccggg tcgcggcctc gacgggtaca ttgtcggtgc
     1981 cgctgcctgt ccgaataatg gcgccgcacc tcgcgagcag cggcagcgta ctctgcgaaa
     2041 cgatccgact tccgccgaag atccagacca cgtcggcgtc cccggccgtc tcggccagct
     2101 cctcgggtgt cgtgcactcc cgcatggcga acgtaatccg ctcggcagcc aggcgctcgg
     2161 tcacccaagc gggcgtcccg ggacagtcta gaccgacgag cgcaaccttg aggctcattg
     2221 cttccgtccc ccggcgggcc accgtcgcgg tgtagcgctc aggatcttgc cgggcgtgct
     2281 tccatcatac gtctgccagg cgttcattcg agtccccgaa ctctttcatg tggatgccgc
     2341 acttatcgag cagcgacagg tagaggctgc acatcttccg gttcggtttg cccagatagt
     2401 ccaggacgcg cccgccttga tctgtccgcc ggcgcccccg atcaggtccc aggcattcgg
     2461 ccgaccgaat tcgcagcgtc acgtttggct ctcgagccac gattataccc ctccggactt
     2521 gggtctggac ctagcgctcg atcacgaata ggtgttgtgc gtgacaggcc tacgcacggt
     2581 ccatacctcg gaggtgaaga agcccaccgc agtggtgtcc agtgggccct caggcatgtc
     2641 cgccaagacg tcagtcggtg tggctaaaag acagtagttc gcttttcctg gtgggggaga
     2701 atagcatcgg ctgaggcgga aatcattcga aattatctca cccggcaaat aaggtcgaga
     2761 gttttcgcga agcccggagg cccaatcggc aaatagggta agaaaaggct aacagcccgt
     2821 ggtggaaacg gttttgcgtg atcctgttag gtacagatga cccgaacaaa agaggatcag
     2881 tcgcacgcat tcgaagctga ggccgacgcc ggaacggcgt gtatgctcgg tgaagcagcg
     2941 ccagagggca aaaa
//
LOCUS       DASRJQ010000586         5568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2059735, whole genome shotgun sequence.
ACCESSION   DASRJQ010000586 DASRJQ010000000
VERSION     DASRJQ010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5568
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2059735"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VFB89_13325"
     CDS             complement(<1..676)
                     /locus_tag="VFB89_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28322.1"
                     /translation="MTYTYKLSRRLARLRVGPVVPLVLLVACSAGTPTDPALNPPVDT
                     GTDGPVALSPRSVTLEGSQGALFKAFESLIPGSSQVTSIEWIATGGTIDASGNYASTS
                     TGSFKVVGRRKGNPHNQPDTSVVIVVPTQPTLVKVTLTPSTATVATGTQRQFSVVGTR
                     SDNTQVQIGVTWSADGGTIDQGGLYTAGRPGTYHVVAKHVTTGLADTAVVTVPPATLT
                     SITLAPT"
     regulatory      complement(744..836)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1169..1726
                     /locus_tag="VFB89_13330"
     CDS             1169..1726
                     /locus_tag="VFB89_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HZM28323.1"
                     /translation="MRCLALNASFEPLTMVPVRRALRLVIEGKAEIVESDGVDVVRSE
                     RLAFPKPAIIRLVRFVHVPRRFRRQVTNTFLFARDGYRCQYCHRSQLELRHRECLTRD
                     HLVPLSRGGDNEWTNCVTACSTCNTKKGNHLPAECGMHPLASPHEPHFVHLSWAVRRL
                     TAQQAKYIRLFYGEAALRALGGIAV"
     gene            1710..2270
                     /locus_tag="VFB89_13335"
     CDS             1710..2270
                     /locus_tag="VFB89_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28324.1"
                     /translation="MVSRFNGEDTLTAPKILLREDQIPPAFPGKTDLAWALISGVGFV
                     LLATNFFDLVGFAAIPRAVGLQQWKISAVARAYDTLPLITLGLLLVLSAGIARGRRRL
                     VLVMVCVLIPFGTLLLITPLLYVSGLVMAWYAGDDPVQRFSLKGGIINRAVWRATVYP
                     LLYIWLGLKGLHWAHTPHPVLGPPPT"
     gene            complement(2317..3504)
                     /locus_tag="VFB89_13340"
     CDS             complement(2317..3504)
                     /locus_tag="VFB89_13340"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HZM28325.1"
                     /translation="MAALVFIAAATLVPHPERVALVEQTPIWCLVCGDLGMVDVLCNV
                     ALFMPLGAGLALLGVRRRQAIALGALLSFSIELAQYVAIIGRDASLSDLITNTIGTTL
                     GAILADSWRAWIFPSRSTARVLAPATALIWLAQVALTGAAVRPALPNTVYWGQRAADL
                     GQFDTFPGRLLDARIGTANLPGHRLTNSAEVRNQLLSGEMVTAVAEPAGLTKGIAPLA
                     SIFDDEQREIVVLAQWKDDLLFRLRARAIDFELRPPAIRLPGVFHNTTGRTVTAAGGV
                     RDNRLIIEANGPTGRRSRVLPLSAQWGWSLLLPFDYAFGPEAGWVSAFWVGCWLWLVS
                     FWLQRTGIKPLRGGALLLLLLLLGLVAIPALFNERATIAECCVGVVGAVLGWRLSSTL
                     RAA"
     gene            3678..4520
                     /locus_tag="VFB89_13345"
     CDS             3678..4520
                     /locus_tag="VFB89_13345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA system polysaccharide deacetylase"
                     /protein_id="HZM28326.1"
                     /translation="MAQPACHVFSVDLEEYFQVLAFERAVPRAQWDSMPSRVEASVNQ
                     LLELLARHSATATFFTVGWVAERKPGLIKRVAELGHEIASHSWWHRRVTALSPSEFRD
                     DVRRCRSVLEDLSGRPVRGFRAPSFSIVPGTEWAFDVLIEEGYRYDSSVFPILRPDYG
                     YPGASPDPYQIRRPAGVLLELPMATTVAFGVRLPAAGGGYFRHLPYALTARALRDHEA
                     RGSAAMFYIHPWEIDTEQPRVPVSPITRFRHYGGLSRTLPRLERLLATFRFVSAESRY
                     EFAA"
     gene            4517..5539
                     /locus_tag="VFB89_13350"
     CDS             4517..5539
                     /locus_tag="VFB89_13350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family XrtA/PEP-CTERM system-associated
                     protein"
                     /protein_id="HZM28327.1"
                     /translation="MTLTVSAFDGVAEVWDGFVRQHEGWTHFHLYGWKRIMENVLEHE
                     CLYLAARDRSGMLQGVLPLVRVRSAVFGHFLVSMPFLNYGGPLGSAEAIRALADHATA
                     LADKDRVKLLEMRSRVPLPLELPVSHRKITVVLDLPEKAPELWSQLGSKLRSQVKRPQ
                     KEGVEMRFGLEEVEPFFAVFARHMRDLGTPTQPKKLFRTVADAFPDTVWFGCGYHRGH
                     PVAGGVAFRWAQEIEMTWASSLTAYNAIAPNMLLYWSFMERAVADRLTLFNFGRCTPG
                     VGTHRFKRQWGSRDEPLWWYQRGRETVATPSPDDPAFAWGPRLWRRLPLPLATALGPR
                     VVRLIP"
BASE COUNT          979 a         1725 c         1778 g         1086 t
ORIGIN      
        1 tggtgggagc cagggtgatc gacgtgagcg tggcgggagg taccgtgacc accgccgtat
       61 cagcgaggcc ggtcgtgacg tgcttggcga ccacgtgata ggtgccggga cgacccgcgg
      121 tgtacagccc gccctggtcg atggttccgc cgtcggcgct ccaggtgaca ccgatttgta
      181 cctgggtgtt atcgctccga gtccccacca ccgagaactg tctttgcgtc ccggtggcca
      241 ctgtcgccgt actcggagtc aatgtgactt tcaccagggt aggctgggtc gggacgacaa
      301 tcacgacgga cgtatcgggc tggttgtgcg ggttgccctt gcgtcggcca accaccttga
      361 acgagccggt gctggtcgat gcgtagttac cgctggcgtc gatggtacca ccggtggcga
      421 tccactcgat ggacgtgacc tggctcgatc ctggtatgag cgactcgaac gccttgaata
      481 gagcgccttg gctgccttcg agggttacgg agcgcggaga aagggcgacc ggaccatcgg
      541 tacccgtgtc cacaggaggg ttgagggctg ggtcggtcgg cgtgccggct gagcacgcga
      601 ccaagagaac aagcggaacc acagggccca ctcgaaggcg ggcaaggcgt cgcgacagct
      661 tgtaggtgta cgtcatcagg caactcctgt ggggtagaga aaccgaaagc cgccagcccg
      721 gagcctcggt agaggctttc gtcgctggcg gcccggctgg cgtgacgatc agattcgatc
      781 gtggtagccc cgaagctctg tgccgccgcc tttcggcggc tttactctga gcagcaatgg
      841 agacggcgcc gatgcaaggc gcctatgaga gttttgcgag ttgtctggca acttctgatc
      901 cacggcgttg gcgcggtgag atgcgttttg cagaatcaat tgccactacg tcagttgctt
      961 cgttgtgatg tctcctaact agacgaacgc accctgtaaa aaagttgagt ggtagcctcg
     1021 aggcggtctt gaatgccgcc gaggagccca gaatctgcaa gaatcgacaa cccttgcacc
     1081 gatacgggtt tggcgtgaca atcgctccct tgccggtatg gggtctgctc ggttagcatt
     1141 tgacggctct tgcggttacg ggaatctcgt gcgctgcctc gcgctcaacg cgtcgttcga
     1201 acccttgacc atggtcccgg tgcggcgggc gctgcgcctg gtgatcgaag ggaaagctga
     1261 gatcgtcgaa tcggacggcg tagatgtcgt ccggagcgaa cgcctcgcct tccccaaacc
     1321 agccatcatt cggctggtca ggtttgtgca cgttccccgc cggttccgcc gccaagtcac
     1381 caacaccttc cttttcgctc gcgacggata ccgctgtcag tattgccatc gaagccagct
     1441 cgagctgcgt caccgcgagt gcctcacgcg cgaccatctg gtgcccctat ctcgcggcgg
     1501 cgataacgag tggaccaact gcgtcactgc ctgcagcacc tgcaacacca aaaagggcaa
     1561 tcatctcccg gcggagtgcg ggatgcatcc attggcttcc ccgcatgaac cacatttcgt
     1621 gcatctctcc tgggcggtcc gccgactcac ggctcagcag gcgaaataca tccgactctt
     1681 ctacggcgaa gcggcgctcc gcgcattggg tggtatcgcg gtttaacggt gaggacactc
     1741 tgaccgcacc gaaaatcctg ctccgtgaag accagatccc tcctgcattc cccggaaaaa
     1801 cagacctggc ttgggctctg atctccgggg tgggcttcgt ccttctggcg acgaattttt
     1861 ttgacttggt gggcttcgcc gcgatccctc gcgcggttgg cctgcaacag tggaagatca
     1921 gcgcggtcgc ccgggcatac gacacgcttc ccctgattac gctcggactg ctgctcgttc
     1981 tgtcggccgg catagccaga gggagacgcc ggctggtact ggtgatggtt tgtgtgctga
     2041 tcccgtttgg gacgctgctt ttgatcacgc ccctgctgta cgtctctggc ctggtgatgg
     2101 cgtggtacgc cggcgacgat ccggtccagc gcttcagcct caaaggtggc atcatcaacc
     2161 gcgctgtttg gcgtgccacg gtgtatccat tgttgtacat ctggttgggt ctcaagggat
     2221 tgcactgggc ccacacgcct catccggtgc tggggcctcc accgacctga tcagacccgc
     2281 gaccggcggt gcgtcaccaa gcgcctgacc ggttgtctat gccgcgcgta gcgtcgagct
     2341 gaggcgccag cccaggacgg ctccaacgac gccaacgcag cattccgcta tggtcgctcg
     2401 ttcgttaaat agggcgggaa tcgctaccag tcccagtagc aacagcagga gtagaagggc
     2461 tccgccgcgc agcggcttga tgccggtacg ctgcagccaa aagctcacga gccagagcca
     2521 gcatcccacc cagaaggccg acacccagcc ggcctcgggt ccgaaggcgt agtcgaacgg
     2581 cagcagcagg ctccatcccc attgagcact caagggcagg actcgcgaac gccggccagt
     2641 gggcccattc gcctcgatga tgagccggtt gtcccgaacg ccgcccgcgg cggttaccgt
     2701 acgccccgtt gtgttgtgaa agacgcccgg gagtcggatt gcaggcggcc gaagctcgaa
     2761 gtcgatcgcc cgtgcacgaa gtcggaacag gagatcgtct ttccattggg cgagcacgac
     2821 gatctcccgt tgctcgtcgt caaaaatgct cgcaagcggg gcaatccctt tcgtaaggcc
     2881 tgccggctcg gcgacggcag tgaccatttc gccgctgagt agctggttac ggacctcggc
     2941 agaattggtc aggcgatggc ccggcaaatt ggccgtgccg attcgcgcat cgagaagtcg
     3001 gccggggaag gtgtcgaact ggccgagatc ggcggcacgt tgcccccagt acacggtgtt
     3061 aggtagcgcg ggtcgcaccg ctgcgccggt cagggcgacc tgagccagcc agatcaaggc
     3121 ggttgcgggg gcgagcacac gagctgtcga acggctgggg aagatccagg cgcgccatga
     3181 atcggccagg attgcgccga gcgtcgttcc gatggtgttc gtgatgaggt cactcagcga
     3241 agcgtcgcgc ccgatgatcg cgacatattg cgcaagctcg atggaaaagc tcaacaacgc
     3301 gcccagcgca attgcttgtc gccgccgaac ccccagcagg gcaagccccg cgcccagcgg
     3361 catgaaaagg gcaacattgc agagaacatc caccatcccg agatcgccgc acacgaggca
     3421 ccagatcgga gtctgctcga ccagggctac gcgttcggga tgagggacca gcgtcgcggc
     3481 ggcaatgaag acgagtgccg ccaaggtgat tacccaggcc gcccagcgcg gcgtttggcc
     3541 acccgcttcg tgctgtttga tgtgtcgccc ccgtcgtggc agagtcgtgt gaatgcctga
     3601 gaagaatgaa cggcaccgaa gatcctcagg acggagtccg ctgacaagcc accgcgtggt
     3661 gttcgtatat ttcgcagatg gcccagcccg cttgccacgt attctcggtc gatctcgagg
     3721 agtatttcca ggtgcttgcc ttcgagcgcg ccgtcccgcg cgcgcaatgg gattcgatgc
     3781 cgagccgtgt cgaggcgagc gtgaatcagt tgctcgagct gctggctcgc cactccgcca
     3841 ccgcgacgtt cttcacagtc ggctgggttg ccgagcgcaa gcctggactg atcaagcgcg
     3901 tggctgagct cgggcatgaa atcgcgtcgc acagctggtg gcatcgccgg gtaacagcgc
     3961 tgagcccgag cgagttcaga gacgacgtcc gtcgctgccg cagcgtgctg gaggatctga
     4021 gcggccgccc ggtgagaggt tttcgcgcgc cgagcttttc catcgtgcct ggaacggaat
     4081 gggcgtttga cgtgctcatc gaggaagggt accgctacga ctcgagcgta ttccccatcc
     4141 ttcggccgga ctacggatat cccggagcta gccccgaccc ctaccaaatt cggcgcccgg
     4201 caggagtgct gctggagttg ccgatggcca cgaccgtcgc ctttggcgtg cggcttcccg
     4261 ccgccggcgg aggatatttc aggcatcttc cttatgccct gacggcccgt gctctgcgcg
     4321 accacgaggc gcgtggcagc gcggcgatgt tctacattca tccgtgggag atcgataccg
     4381 agcagccgcg tgttcccgtt tcgccgatca ctcggttccg acactacggc ggcttgtccc
     4441 gaacccttcc ccgactcgag cgcctgctcg ccacatttcg attcgtgtcg gcggaaagcc
     4501 gctacgaatt cgccgcatga cacttacggt ctccgccttc gatggggttg ccgaagtctg
     4561 ggatggcttc gtgcggcagc atgagggctg gacccacttt cacctgtatg ggtggaagcg
     4621 aatcatggaa aacgtgctgg agcacgaatg tctgtacttg gcggcccgcg accgttccgg
     4681 aatgttgcag ggcgtgctgc cgctggttcg agtacgcagc gcagttttcg gccactttct
     4741 ggtctcgatg cctttcctca actacggagg accactcggc tcagcagagg cgattcgcgc
     4801 cttggcggac cacgccaccg cactggctga caaggaccgg gtcaagctgc tcgagatgcg
     4861 aagccgcgtg ccgctacccc tcgagctacc cgtatcgcac cgcaagatca cggttgtgct
     4921 cgatctcccg gagaaggccc ctgagctctg gagccagctc ggctcgaagc tgcggagcca
     4981 ggtcaagcgg ccccagaagg agggcgtaga aatgcggttt ggccttgagg aggttgagcc
     5041 cttttttgcc gtcttcgctc gacacatgcg tgatctgggt accccgacgc aacctaagaa
     5101 gcttttccgc accgtggccg acgcatttcc cgataccgtc tggttcggct gcgggtacca
     5161 ccgtggccat ccagtggccg gtggcgtcgc gtttcggtgg gctcaggaga tcgagatgac
     5221 ttgggcatct tccctgacgg cctacaacgc gatcgcgccc aacatgctgc tttactggtc
     5281 gttcatggaa agggcggtag cggaccgctt gactctcttc aatttcgggc gctgcacgcc
     5341 tggtgtcggc acacatcgtt tcaagcggca atgggggtcg cgcgacgagc cgctgtggtg
     5401 gtatcagcgg gggcgagaaa ccgtcgccac tccctcgccg gacgatcccg cgttcgcgtg
     5461 ggggcctcgc ctctggcgcc gtctgccgct tccgcttgcg acggcgctcg gtccgcgcgt
     5521 agttcgcctg atcccgtgag gctgcgagcg cagccaccgg ttcattcc
//
LOCUS       DASRJQ010000587         3180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_655718, whole genome shotgun sequence.
ACCESSION   DASRJQ010000587 DASRJQ010000000
VERSION     DASRJQ010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655718"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VFB89_13355"
     CDS             <1..360
                     /locus_tag="VFB89_13355"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZM28328.1"
                     /translation="PDPAIIDYTASKAALTNFSKAVSKEVGPRGVRVNTVSPGPVETE
                     LWLGPQGVAATVAKATRTTPAQAREQVVQSQGGFATGRFTRPEEVADLVLFLASDRAA
                     NITGSDFVIDGGLIKTL"
     gene            404..910
                     /locus_tag="VFB89_13360"
     CDS             404..910
                     /locus_tag="VFB89_13360"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZM28329.1"
                     /translation="MGEPKSAANETDISRDQLAELLNEDLSREYQAIIAYVVYSQVLS
                     GAEYMDIATQLEVHAKQELDHALIISRQIDYLGKMPAVTPKPVRTSKKAREMLRYDLE
                     NENETIRNYRDRIRQCEALGEYAMAEQIRQILIQEQDHQIDLATALGEEVPDVNSGPR
                     GRALRRRR"
     gene            complement(1198..2832)
                     /locus_tag="VFB89_13365"
     CDS             complement(1198..2832)
                     /locus_tag="VFB89_13365"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HZM28330.1"
                     /translation="MLLSLTLSLLLQSAAPSGDTLTAAQWRDDLHFMAAEMARLHRKL
                     YHEVTREQFDSAVADLDHRIPMLSREQILVGFARIVALVGDGHTHLGLPWDSAAGFGR
                     YPLALYRFTDGFYVTGADSTHADLVGGSLVSIGGQSVDSLFNAITPLISRDPGNRMWY
                     QLYAGPYMATPEVLQGLGLASKTGQATFVVRRDGEERAVELARGPRPSAMNYNGWVTG
                     GGWVTMHDGVKAPGPLWARHLDDPFWVKYLSSARAVYVQYNAVQDGEKETVAHFFGRV
                     CAEASRRRADRMIIDLRLNGGGDNTLNAEVVKTIMRTSPINRRGHLFVIIGRNTFSAA
                     QNLVNSLERYTDAIFVGEPTGANPNLFGDAVPVRLRHSGVVVYVSTLWWQDLDPRDKR
                     PWTAPEIAAEMTSADFRAGRDPALEAVLRWRSEPNLADQMLAALRQGDTTRAFARYRA
                     YRDDPRHVYADTDRLLRDAALRAAEAGRLPEALAIVEFNVREHPRSSDAQAYLAEGYA
                     RAGLKDQAIAGFERAVALDSTNSFALIRLSQLREGS"
BASE COUNT          614 a          987 c         1024 g          555 t
ORIGIN      
        1 ccggacccag ccatcataga ctataccgcg tcaaaagccg ctctcaccaa cttctcgaag
       61 gcggtctcca aagaggtcgg gccgcggggc gtacgcgtga ataccgtgag ccctgggccg
      121 gtcgagacgg agctgtggct cggcccgcag ggggtcgccg ctaccgttgc caaggctacg
      181 cggacgactc cggcccaggc tcgggaacag gtcgtccaga gccagggcgg cttcgccacc
      241 ggtcgcttca cccgaccgga ggaggttgct gatctggtgc tgttcctggc gagcgatcgg
      301 gcggcgaaca tcaccggttc ggacttcgtc atcgacggcg ggttgatcaa gacgctgtaa
      361 gagcacaagc cttggaacgc tttcagtgaa agggtgggat caaatgggtg agcccaaatc
      421 agcggcaaac gaaaccgata tttcccgcga ccagcttgcg gagctgttga acgaagatct
      481 ttcccgtgag tatcaagcca tcatcgccta cgtggtctat tcccaggtgc tcagcggcgc
      541 tgaatacatg gacatcgcga cccaactcga ggtccacgcc aagcaagagc tcgatcatgc
      601 cttgatcatc tcgcgccaga tcgactatct cggcaaaatg ccggcggtta ctcccaagcc
      661 ggtgcgcacg tcaaaaaaag cgagagagat gctccggtac gatctcgaaa atgaaaacga
      721 gacaatccgg aattaccgcg atcgcatcag gcagtgtgaa gcgctcgggg agtatgccat
      781 ggcagagcag attcggcaga tcctcattca ggagcaggac catcaaatcg atctggctac
      841 tgcccttggc gaggaagtcc ccgatgtgaa tagcggtccg agagggcgtg ccctgcgccg
      901 acgtcgctga cctttggcgt tcgcgggtaa gcccggtcca actttcagcc aactccctcg
      961 ccctgcaccc gtatgaaaac catgaccgtt cggcgcaaag gacgccgatc cggaagagga
     1021 cttcggacat ttcgaggacc agcgggagac caacatcgaa ctactgcgcg ggctgccggc
     1081 ggaggctggg aatcgcaagg cgctgcaccg cgcgcgcaag tacctggcgg gcgcgggccg
     1141 ctgggcgagg attacaagct gaagccatga tgattcgcaa cctgctggct ctcactttca
     1201 cgatccctcc cgcaactgcg agagcctgat caacgcaaag ctgttggttg aatccagggc
     1261 gacggctcgc tcgaacccgg cgatggcctg gtccttcaga cctgcgcggg catacccttc
     1321 agccagatac gcctgtgcat cggaggatcg gggatgctct cgcacgttga actccacgat
     1381 ggccaatgct tcaggcagcc gtcccgcctc cgcggcccgc agggcagcgt cgcgcagcag
     1441 gcggtcggtg tcggcgtaaa catggcgggg atcgtcccgg taggcgcgat agcgcgcgaa
     1501 cgcgcgggta gtatcgccct gccggagggc cgcgagcatt tgatcggcaa ggttcggctc
     1561 gctccgccac cggagcacag cctcgagcgc cggatcgcgc ccggcgcgaa agtctgcgct
     1621 cgtcatctcg gcggcgatct cgggcgcggt ccatgggcgc ttgtcgcgag gatccagatc
     1681 ctgccaccag agggtcgaca cgtagacgac cacgccgctg tgccgcagtc gcaccggcac
     1741 ggcgtcgccg aacaggttgg ggttcgcgcc ggtcggctcg ccgacgaaga ttgcgtcagt
     1801 gtaccgctcc agcgagttca ccagattctg agccgcggag aaagtgttgc ggccgatgat
     1861 cacaaagaga tgcccgcgac ggttgatcgg tgaagttcgc atgatggtct tgacgacctc
     1921 ggcgttgagc gtgttgtcgc ccccgccgtt cagccgcagg tcgatgatca tgcggtccgc
     1981 tcgacgccgg ctcgcctcgg cgcagacccg cccgaagaaa tgagcgacgg tctccttctc
     2041 cccgtcctgc accgcgttgt actgcacgta gaccgcgcgc gcggaggaca gatatttcac
     2101 ccaaaagggg tcatcgagat ggcgggccca aagcggaccg ggggccttca cgccgtcgtg
     2161 catcgtcacc cagccgccgc cggtcaccca cccattgtag ttcatcgcgc tcgggcgtgg
     2221 gcctcgcgcc agctcgaccg cgcgctcctc accgtcgcgc cgcacgacga aggtcgcctg
     2281 tcccgtcttt gaagcaagcc cgagaccttg gagcacctcg ggcgtcgcca tgtagggacc
     2341 cgcatagagc tgataccaca tccggttccc cgggtcgcgc gatatgagcg gggtgatggc
     2401 gttgaagaga gagtcgacag actgcccgcc gatactgacg agcgaaccgc ccaccaggtc
     2461 ggcgtgggtc gagtcggctc ccgtgacgta gaagccgtcg gtgaaccggt acagcgcgag
     2521 ggggtaacgt ccgaatcccg cagccgaatc ccacggcaac cccagatggg tgtgaccgtc
     2581 gcccaccaac gccacgatgc gtgcaaagcc gaccaggatc tgctcgcgcg acagcatcgg
     2641 aatgcgatgg tcgagatcgg cgacagcgct gtcgaattgc tctcgggtca cctcgtggta
     2701 aagcttgcgg tgcagccgag ccatctcggc cgccatgaag tgcagatcgt cacgccattg
     2761 ggcggcggtg agcgtgtcgc cagaaggcgc ggcgctctgg agcaagagcg agagcgtgag
     2821 cgagagtagc atgagactga tctccgggga cgtctcgtgg gagtcagacg gaccgaagtc
     2881 tctacggctc cctccagggc aggtttcatt ctgctacctg tttgaccgct cgcgctggtg
     2941 gccggtcagt tgaatttccg tctgtccaac atggttatgg ccgggctgtg gggctggcgg
     3001 aataggtaag ctcacccgcg gccttgtcgg gaggttcgac atgagcaagc aggacagcga
     3061 gttggtcgtt tcctacctga cgctcagaca gatgatcggc tggatcggac tactgatgcc
     3121 attcttcgtg cgcgtggggg cgtttcttcg cgagggcatt cgctcgaccg attcgatcag
//
LOCUS       DASRJQ010000588         3710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2237048, whole genome shotgun sequence.
ACCESSION   DASRJQ010000588 DASRJQ010000000
VERSION     DASRJQ010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2237048"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="VFB89_13370"
     CDS             <1..996
                     /locus_tag="VFB89_13370"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HZM28331.1"
                     /translation="TAAALAGGTAIAAALPARWANAAEFNYKWGTNVPETHPLNVYAR
                     KAAEAIKKDTNGRFDLQLFPNNQLGGDTDMFSQLRSGALECFTLSGVNVLSTLIPSAS
                     IYGLGFAFQNYQTLWNALDGKLGAHLRAQINKAGLIVMDKIWDNGFRQITTSTKPIVK
                     PADLVGVKIRVPVSALWTSLFKSLGASPASINFSEVYSALQTKVVDAQENPPAIISAA
                     KLYEVQKYCSLTNHMWDGWWFLVNRRAWERVPRDVQEIVAKNINAAAVAQREEVAKQN
                     DGLKADLAAKGLVFNDVDPAPFQEVLRKSGFYVEWKGKFGEEAWQLLEEATGKLA"
     gene            1699..2913
                     /gene="ltrA"
                     /locus_tag="VFB89_13375"
     CDS             1699..2913
                     /gene="ltrA"
                     /locus_tag="VFB89_13375"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HZM28332.1"
                     /translation="MQRRRAPSRNWDWVEAGIWTERMLSALDAGVKGGKWYSLIDKVY
                     THDTLELAWAKVEANKGAAGVDGQSVERFAAKADVYLTELSTALREDRYRPQAIKRVE
                     IPKGDGRMRPLGIPTVKDRIVQQAVRLVIEPIFESGFCDGSYGFRPERGSRDALREVD
                     RLLKAGYTHVVDADLQSYFDTIPHERLMARVEDRVSDGRVLDLIRGWLQADILQDLER
                     WTPTEGSPQGAVISPLLANIYLDPLDRLMAEHGYPMVRYADDFVIPTRSHAEAEAALA
                     LVRGWVAANSLTLHPEKTRIANCRKKGNGFEFLGYRFERGRRHVRKRSLDKLKETIRV
                     KTRRTRGQSLKVVVDDLNRSLRGWFGYFKHLPEHLPRTGSDDPAATARHAPQTKRTAG
                     HRQGAARSPAMA"
     gene            complement(3115..>3710)
                     /locus_tag="VFB89_13380"
     CDS             complement(3115..>3710)
                     /locus_tag="VFB89_13380"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZM28333.1"
                     /translation="LAAIQDAMGNNAIVVTDGGDFLSFARVGLSAPTMLDPGPFGCIG
                     IGVPYGIAASLACPDRPVIVATGDGSFGFNAIEIDTAVRHKAPVVIVVANNGAWQIEV
                     HDQTVTHGKVVGTRLQFSDHAAMARAFGMHGERVEKPEALAPALDRAFANRPALVDVV
                     VTPEAVSSDGKTGLAWVPDLQPLELWDKAERKWREGK"
BASE COUNT          727 a         1109 c         1281 g          593 t
ORIGIN      
        1 accgcagccg ctttggccgg cggcaccgcg atcgcggccg cgctgccggc ccgatgggca
       61 aatgcggccg agtttaatta caagtggggc accaatgttc cggaaacgca tccgctcaac
      121 gtctacgcgc gcaaggcggc ggaagcgatc aagaaggata ccaacgggag gttcgacctg
      181 cagctctttc cgaacaacca gttgggcggc gataccgaca tgttctcgca gctcaggtcc
      241 ggcgccttgg agtgctttac gctttccggc gtgaatgtgc tctcgacgtt gataccgtcc
      301 gcttcgattt acggcttggg cttcgcattc cagaactatc agacgctctg gaacgcgctt
      361 gacggcaaat tgggcgctca cctgcgagcc cagatcaaca aggccggcct gatcgtgatg
      421 gacaagatct gggacaacgg ctttcgccag atcacgacca gcaccaagcc gatcgtcaaa
      481 cccgccgacc tggtgggagt gaagatacgc gttcccgtca gcgcgctatg gacgtcgctg
      541 ttcaagtcgc tcggtgcctc gccggcaagc atcaacttct cggaggtcta ctcggccttg
      601 caaacgaaag tggtcgacgc gcaggaaaat ccgccggcca tcatctcggc cgccaagctc
      661 tacgaggtgc agaagtattg ctcgctgacc aatcatatgt gggacggctg gtggttcctg
      721 gtgaaccgtc gtgcctggga acgcgtgccg cgcgacgtcc aggagatcgt ggcgaaaaac
      781 atcaatgcgg ccgcggtggc gcagcgcgaa gaggtcgcca agcagaatga cggcctgaaa
      841 gccgacctcg ccgccaaggg gctggtattc aacgacgtcg atccggcgcc gttccaggag
      901 gtcctgcgca agtcgggctt ctacgtggaa tggaagggca agttcggcga ggaggcctgg
      961 cagctgctgg aggaggccac cggcaagctg gcttaacgcg tcatccccgc gaaagccgac
     1021 aggcaccagc ctaattcgcc agcctgctcc tggctgtcat cccggccaag cgactccggc
     1081 ctcgagccgg gggagcgcga gccgggaccc atgtagccct gcttggaaac tgtgcgcgca
     1141 gcgccggggt aacgtggatc ccggctcctg ccccggctca aggccgggtc ggccgggatg
     1201 agtgcgtccg tcagtctggg ttgtcagcat agtgggagtc tatgccatgt gagtcaccgc
     1261 acatgtagcc gaacgcaact gcgtcgacca accgcgcaaa tgcgggaggc ggggtgggga
     1321 gcagccggag gcgaaccgac agcccgtagg aaaacgaacc cgattcggcg gagtgcagtg
     1381 gcgagcctgc tcattcatgg cgaagcctgg gaccgcaagg accggccggt tgcacgaggt
     1441 gataggcacg tgacgaccgg gctgcatagc gtggttaggg acgggatgcc gggaaggcaa
     1501 agcagacgga acggagagac ctgctgcgcc ggcgaagcgg agcaggagtc agagcccgca
     1561 tagtaccgag gcggccgggt cggtgcgcga gaggatcggg tagcaaaacc gatccgaggg
     1621 aaggcagggc aggaaggtgg aagcgtgcga tcatatcagg gcagagcagc gccggcagtg
     1681 ccgcagaagc taagcaaggt gcagagacgc cgagccccca gccgcaactg ggactgggtc
     1741 gaagccggga tatggacgga gcgcatgctg tcggcgctgg atgccggcgt caaaggtggc
     1801 aagtggtact cgttgatcga taaggtgtat acgcacgaca cgctggaact cgcctgggcc
     1861 aaggtcgagg cgaacaaggg cgcggcgggc gttgacgggc aaagcgtgga gcggttcgcc
     1921 gcgaaggctg acgtctatct gacggagctg tcgacggcgt tgcgggagga caggtaccgc
     1981 ccgcaggcga tcaaacgggt cgagattccg aagggcgatg gcagaatgcg accgctgggc
     2041 atccccacgg tcaaggatcg catcgtccag caggcggtcc ggctggtgat cgagccgatc
     2101 ttcgagagcg ggttctgcga cggcagctac ggcttccgtc cggaacgcgg gagtcgtgat
     2161 gcgctgcgcg aggtcgatcg gctgctcaag gctggctaca cccatgtggt ggatgccgac
     2221 ctgcagtcgt acttcgatac gattccgcac gagcgcctga tggcgcgggt cgaggaccgg
     2281 gtcagcgatg gccgcgtgct cgatctgatc cgcggctggc tgcaagccga catcctgcaa
     2341 gatctggagc gatggacacc gactgagggc tcaccgcaag gcgcggtgat tagcccgctg
     2401 ctggctaaca tctatctcga tccgttggac cgactgatgg ccgagcacgg atacccgatg
     2461 gtgcggtatg cggacgactt cgtcattcca acccggagcc acgcggaggc cgaagcggcg
     2521 ctcgcgctgg tcaggggctg ggtggcggcg aacagtctca ccctgcaccc cgagaagacg
     2581 cggattgcga actgccgcaa gaaggggaac gggtttgagt tcctcggcta ccggttcgaa
     2641 cgcggccgtc gtcacgtacg caagaggagc cttgacaagc tgaaagagac gatccgcgtg
     2701 aagacgcggc ggacccgagg gcaaagcctg aaggtcgtcg tcgacgatct caaccggtcg
     2761 ctcagaggct ggttcggcta cttcaagcac ttacccgagc atcttcctcg aactggatca
     2821 gatgatccgg cggcgactgc gcgccatgct ccgcagacaa agcggacggc cgggcatcgg
     2881 caaggcgcgg ctcgatcacc agcgatggcc taatgcctac ttcgcgaatg ctgggctgtt
     2941 cgcgcttcac gcggcctggc aatcagcgag acagtctcga tgacagaaac caccaactgg
     3001 agagccgtat gcgggaaaac cgcccgtacg gttcggaggg cggggtcggc gtaagccgat
     3061 cccgacccct atcacagcgc gccgctcaga cgttcgtcgc aaacggcggg cgcgctattt
     3121 tccctcgcgc catttccgct ccgctttgtc ccagagctcg agcggttgca ggtccggcac
     3181 ccaggcaaga cccgtcttgc cgtcggaaga aacagcttcg ggagtgacga cgacgtcgac
     3241 cagggcgggg cggtttgcaa acgcgcggtc gagcgccggt gccagcgcct ccggcttttc
     3301 gacgcgctcg ccgtgcattc cgaacgcgcg cgccattgcg gcgtgatcgg agaactgcag
     3361 cctggtgccg acgaccttgc cgtgggtgac ggtttgatcg tgcacctcga tctgccatgc
     3421 gccgttgttg gcgaccacga tcacgaccgg ggccttgtgc cggaccgccg tgtcgatctc
     3481 gatggcgttg aatccgaacg agccgtcgcc ggtggcgacg atgacggggc ggtcggggca
     3541 ggcgaggctc gccgcgatcc cgtacgggac gccgatgccg atgcagccaa acgggcccgg
     3601 atcgagcatg gtcggagccg agaggccgac ccgggcaaag ctcaggaagt cgccgccgtc
     3661 cgtgacgacg atcgcattgt tgcccatggc gtcctggatg gccgcgagga
//
LOCUS       DASRJQ010000589         2038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2392627, whole genome shotgun sequence.
ACCESSION   DASRJQ010000589 DASRJQ010000000
VERSION     DASRJQ010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2392627"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..1118)
                     /locus_tag="VFB89_13385"
     CDS             complement(129..1118)
                     /locus_tag="VFB89_13385"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HZM28334.1"
                     /translation="MSSPVAPVTLTALQQPVRSELERVQRDLRRIIEADFQLIREVNS
                     HLLQMQGKLFRPTILLLTDQATGAATTHAVTLAAVVELIHLATLVHDDSVDHSVLRRG
                     MPTVNSLFSHQVSVIMGDYLYSRAVIELVRLNQFEALRVLSRVTNEMTVGEMRQLLAH
                     DPLSFAEDQYDLLIRAKTASLLSGAAELGALDAPAAQRAAAQRFGEALGMAFQIVDDL
                     LDYTADATALGKPSGSDLREHKVTLPLIHALPRMTEAERRNVGALLQNAEPSDSQIAE
                     VIGAVAQAGGLDYARERAQRLGQQADAELDCLPSSPARDALRASITYVLDRRS"
     gene            complement(1115..>2038)
                     /locus_tag="VFB89_13390"
     CDS             complement(1115..>2038)
                     /locus_tag="VFB89_13390"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM28335.1"
                     /translation="GGMIARLIRLEPDDISHYQKLVELAYRTGSRGRMLEAYLALAAA
                     LRRIGETEKAIAVYQRVLDHEPGNRAAERALAEMTEPVVEEEPPPKKSAKAHPAGAAP
                     SAPAPPVAAPSSPTPPAATPAGTGAPREDFVDLGSLVLGGPVRPRDTRMRVEGATPTD
                     DEQKNFEETLAEFKRGIEQNLDVDDYQTHYDLGVAFKEMGLLDEAIAEFQKALRAPEG
                     RLRTSEALGVAFFDKGQFGIAETVLRRGVDGEAGGDEAKIGLLYWLGRAAEAQGKVPE
                     ALTAYERALAVDIRFMDLGERVPRLTAGRNA"
BASE COUNT          344 a          674 c          664 g          356 t
ORIGIN      
        1 gtgaaaaagg tcttggctgc gctgtcaggg agccactggc gcaggaacga ggtgagaaag
       61 ccccccgcaa tgaacccggt gaccaacacc ccgatgtaga atccgggccg atgaggtccg
      121 ctggccattc agctccgccg gtcgaggacg taagtgatgc tcgcccgcag ggcatcccgg
      181 gcgggcgagg atggcaggca gtccagctca gcatcggcct gctggccgag ccgctgggcc
      241 cgctcgcggg cgtaatccag tccgccggcc tgagcgacgg caccgatcac ttcggcgatc
      301 tgggagtcgc tcggctcggc gttctgaagc agggcgccga cgttccggcg ctccgcctcg
      361 gtcattcggg gcaacgcatg tatcagcggc aaggtgacct tgtgctcgcg caggtccgag
      421 ccggagggct tgcccagggc cgtcgcatcc gcggtatagt ccagcaggtc gtccacgatc
      481 tggaaagcca tccccaacgc ttcgccgaac cgctgcgcag cagctcgctg cgcagccggt
      541 gcgtccaacg cacccaactc cgccgcccct gagagtagtg aggcggtctt ggcgcggatc
      601 aggagatcgt actggtcttc tgcgaaggac aggggatcgt gggccagcaa ctgacgcatc
      661 tcacccacgg tcatttcatt ggtcacccgg ctcagcactc gcagagcttc gaactggttc
      721 agccgaacca gctcgatcac cgcgcgcgag tacagatagt cgcccatgat gaccgacacc
      781 tggtggctga acagggagtt gaccgtgggc atgccccggc gcaggacgga gtggtccacc
      841 gagtcgtcat gcaccagagt tgcgaggtga ataagctcga ccaccgctgc cagagtcacc
      901 gcgtgggtcg tggcggcgcc ggtcgcctga tcggtgagaa gcaggatggt ggggcggaac
      961 agtttaccct gcatctgcag caggtgagag ttgacctccc gaatgagttg aaaatcggct
     1021 tcgatgatcc ggcgcagatc ccgctggacc cgttccagct cactccggac gggttgctgc
     1081 agcgccgtca gcgtcaccgg ggcgacaggc gaactcatgc gttgcgtccg gcggtgagcc
     1141 tggggacgcg ttcgccgaga tccatgaagc gaatgtcgac cgcgagagcc cgctcgtatg
     1201 cggtaagcgc ctccggcact ttcccctgag cttcggccgc gcggccgagc cagtagagca
     1261 ggccgatttt ggcttcgtcg ccaccggctt cgccatctac cccgcgccgc agcacggttt
     1321 cggcgatccc gaactgacct ttgtcgaaga aggcgacgcc caatgcttcg gacgtccgca
     1381 gccgtccttc gggggcgcgg agcgctttct ggaattccgc gatggcctcg tccaggagac
     1441 ccatctcctt gaaggcaacg ccgaggtcgt agtgcgtctg atagtcgtcc acatcgaggt
     1501 tctgctcgat gccgcgtttg aactcggcca gcgtctcctc gaaattcttc tgctcatcat
     1561 cagtcggggt tgccccttcg acccgcatcc gggtatcccg cgggcgtacc ggtccgccca
     1621 gcacgagtga gccgaggtcc acgaaatcct cccgcggcgc cccggtacct gccggcgtgg
     1681 cagcaggtgg agttggactg gaaggtgcgg ccaccggagg cgccggcgca gaaggcgccg
     1741 caccagcggg atgcgccttc gcggacttct tcggcggcgg ctcctcttcg accaccggtt
     1801 cagtcatttc agcgagcgcc cgctccgccg ctcggttacc cggctcgtgg tccagcacgc
     1861 gctgatagac cgcaatggcc ttctccgtct caccgatacg gcggagcgcc gccgcgagcg
     1921 cgagatacgc ttccagcatc ctgccgcggc tgcccgttcg ataggccagc tcgaccagct
     1981 tctgatagtg actgatgtcg tcgggctcta ggcggatgag cctggcgatc atgccgcc
//
LOCUS       DASRJQ010000590         4760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1786588, whole genome shotgun sequence.
ACCESSION   DASRJQ010000590 DASRJQ010000000
VERSION     DASRJQ010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1786588"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="VFB89_13395"
     CDS             complement(<1..1101)
                     /locus_tag="VFB89_13395"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZM28336.1"
                     /translation="MVIAAVLALLSQGPGDSVSLRDALARARAARGQVTATAAQVAEA
                     RAALRSAGAITNPTVSYSHSGAVPKEHLLVDQSLEYLLRRSPERAGAESRILGAQADS
                     SRSMAELVRDVRSAFYGALAADISERLTAAQSVQADSVAGIAAARLRAGDISMLEQEQ
                     AGLEAARARQALSVAREGARVAEAALGRAIAWTALDLPHPTGSMETDLARPVPELPPT
                     DSLPAVRIAVAESAATAALARSASVARIPMPTLQGGAEWNDPTQPGTLAVLGIAVPLP
                     LWQSGGGPAAEAEARARAAAALSGETRLEAARGLRDARNRIAESGQRARFARDSMLPA
                     AARLRARAVRAYQAGETGILPVLDALRSEREVT"
     gene            1460..4279
                     /locus_tag="VFB89_13400"
     CDS             1460..4279
                     /locus_tag="VFB89_13400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZM28337.1"
                     /translation="MRIVRTVAHAGWQVGLFVLAAGSLNGQERRSGLLTGQVSDPMDN
                     ALPGATIRVLERGLSATSDREGRFIFSGLPAGEYRITVRYIGFKPDTLPVVVQSGGES
                     RVKVVMQPLPASLAEITVTAARPRGEAQAMSAQKSNDNIVNVLPAEIITSLPNTNVAD
                     AVGRLPGVTLERDEGEGKYVQVRGTDPRLSNLTIDGVHIPSPEGGVRNIKLDVIPSDL
                     VGTIELYKTLSADQEADAIGGSVNLVTKTPGDRPFATVSGQAGYTNTQQGRGLYQFTG
                     TAGGRFGSDKRFGAILGGTYDWNGRGIDDIEPSVGTNDFGKGPVPVVSGVDYREYRYD
                     RSRYGFGGGLDYRLGAGASLYLRGLFADFKNYGTRWVTSPSAGDFLTQTTTDSNGTVS
                     RTVQDRTPNEQIYSLVGGGRHHISRAILEYSGSYSHSRQDRLRQRTSEYAGPSDVAYA
                     IDQTDPYKPTFNVSNGVNLEDPTAFSLDNSSTADERAAARAIAGRLDLTVPIRVNGAD
                     LALKFGGKIRDDRKTQSVADSDYDATTAPALNLAQVESSFFNPNYYFNYFTHTGYVLG
                     PVVDFDANNAMLAADPTALELNVNDTHADSDPNNFTAQERVYATYGMATLRAGHLHVR
                     AGVRLEATRADYTGYVVHFNDAGDWLGTSPVSGSQSYTDVLPSTELRYELDDNTNLRA
                     AYGEGISRPNYADLPPFRVQNDEKAEIDIGNPNLIPTRANNFDLLLEHYFTTVGVLWG
                     GFFYKNLKDPIYEAVETQITSGPDVGFTQSQPINGPSAYVWGIELAWQQHLTFLPGVL
                     SGLGILANYAYSYSKATVPGRADQPSLERQAPETWNLGLSYDRGGFAGRIAATHNGAS
                     IFAYNFQDGADGGLAGPNGDTYLYPRTQLDAQAGYRLRNGMEFFVQVLNITNEVFGFY
                     NGSEQFPIQREYYGWSTSLGMRLTR"
     gene            4313..>4760
                     /locus_tag="VFB89_13405"
     CDS             4313..>4760
                     /locus_tag="VFB89_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28338.1"
                     /translation="MRGASILGVVTAFAVGGAPASPRLEPPYHLIRRVVLGGQGKWDY
                     LTMDPVGRRVFIAHGTEVLVVNADSGSVVGKIPDTPGVHGIALAQSLGRGFISNGADS
                     TVTVFDLTSLKALDRITVTGRKPDAIVYDSVSGRVFTFNGGSANATA"
BASE COUNT          892 a         1512 c         1526 g          830 t
ORIGIN      
        1 cgtcacctca cgttcggagc ggagagcgtc gagcacggga aggatgccgg tctcaccggc
       61 ttggtacgct cgaaccgccc gcgcgcgcaa cctggccgcc gccggcagca tcgagtcgcg
      121 cgcgaagcgg gctcgctgac cgctctcggc gatccggttg cgagcatccc gcaagccgcg
      181 agctgcctcg aggcgggttt cacccgagag tgccgcagcc gcccgagcgc gtgcctcggc
      241 ctcggctgca gggccgcctc cgctttgcca cagcggcaac ggtacggcaa tcccgagcac
      301 ggccagtgta cccggctgcg tcgggtcgtt ccattcggct ccgccttgca gcgtgggcat
      361 gggaattcgg gcgacggacg cactacgggc gagtgcagca gtcgcagcag attccgccac
      421 cgcaatgcga acggcgggca ggctgtcggt cggaggcaat tcgggcacgg gccgagccag
      481 atcggtctcc attgatccgg tgggatgagg gaggtccaat gccgtccaag cgatggcgcg
      541 gcccaatgcc gcctctgcga cgcgcgcccc ttccctcgcg accgagagag cctggcgcgc
      601 acgggcagcc tccagtcccg cttgctcctg ctcgagcatc gaaatgtcgc ctgcgcgaag
      661 gcgggcggcc gcaatgccgg cgacggagtc ggcctgcacc gactgggcgg cggtgaggcg
      721 ctccgaaatg tcggccgcca gtgctccgta aaacgcgctg cgcacgtcgc ggaccagctc
      781 ggccatcgag cggctggaat cggcctgggc gccgaggatg cgcgactccg ctccggcacg
      841 ctcaggactc cgtcggagca gatactcgag cgactgatcg acgagcaggt gctccttggg
      901 tactgctccg ctgtggctgt acgagaccgt gggatttgtg atagcgccgg cgctccgaag
      961 cgcggcgcgt gcctccgcca cctgcgccgc tgtcgcggtc acctggcccc gcgcggcccg
     1021 cgcgcgtgcc agagcgtcgc gcagcgacac agaatcgccg gggccttggc tgaggagcgc
     1081 caggacggct gcgatcacca tgctcggtcc gcaactgtca gggctcgcgg accactggcg
     1141 ttgtagctct gcaatgcaga gtgcaccgtt gaaatatgga agtaggaatg ggttagtccg
     1201 ccgtgtcagg ccgcacacgg ggctggttcg cgcgcctgcg tgctcggaac cctagcgcgc
     1261 ggaagtgacc acagtatgac aaggtctgtc atgctgctgt cattcagtct tgcgagcttg
     1321 cttggcctct cgctacgaac cgacgttcgc tgcggtgggc ttcctcccgt gttactcgat
     1381 ggtgacctcg tgatgatatc agcgacacgc gcctcggaga ttatggcggg tttttgccgc
     1441 aactcttcga ggagggccta tgcggatcgt gcgtacggtc gcgcacgcgg gatggcaagt
     1501 gggtctgttc gtgctggcgg ccgggtcact gaacgggcag gagcgtcgct ctgggttgct
     1561 caccggccaa gtgtcggatc cgatggataa cgccctccct ggcgctacca tccgagtgct
     1621 cgagcgcggg ctgagcgcga cttcggatag agaaggccga ttcatcttct cggggctgcc
     1681 agcgggagag taccgaatca ctgtgcgcta catcggtttc aagcctgaca cgttgccggt
     1741 cgtcgtccag tcggggggcg agtctcgggt gaaagtcgtc atgcagccgc tgccggcatc
     1801 gctggccgag atcaccgtga cagccgcgcg gccgcgaggc gaggcgcagg caatgagcgc
     1861 acagaagagc aacgacaaca tcgtcaacgt tttgcctgcc gagataatta ccagcctccc
     1921 caacaccaac gtcgccgatg ccgtgggcag gcttccgggt gtgactctcg agcgggacga
     1981 gggcgaaggg aagtatgtcc aggtgcgagg gaccgacccc cgactgtcga atctcactat
     2041 cgatggagtc cacattccgt cgccagaagg tggagtgcgg aacattaagc tcgacgtcat
     2101 tccctccgat ctggtcggga ccatcgagct gtacaagacc ctgtcagccg accaggaggc
     2161 agacgcgatc ggcggctctg tgaatctcgt gaccaagacg ccgggcgatc gaccgtttgc
     2221 aactgtttcc ggccaggctg gctacaccaa cacccagcag ggccggggtt tgtaccagtt
     2281 taccggtacc gcaggcgggc ggttcggcag tgacaaacga ttcggggcaa ttctcggcgg
     2341 cacatacgat tggaatggaa ggggcatcga tgacatcgag ccgtcggtgg gtacgaacga
     2401 tttcgggaag ggtccggtgc ctgtggtcag cggggtggat taccgagaat accgctatga
     2461 ccgcagccgc tacggcttcg gaggcggact cgactatcgg ctgggcgccg gggcttcgtt
     2521 gtatctgcgc gggctgttcg ccgacttcaa gaactacggc acgcgatggg tcaccagccc
     2581 gtcggcaggg gatttcctga cgcagaccac cacggacagc aacggcactg tctcgcggac
     2641 cgtacaggat cggacgccga atgagcagat ctacagcctg gtcggtgggg ggcggcacca
     2701 tatctcccgc gcgattctcg agtatagcgg gtcatattcc cactcccgcc aggatcggct
     2761 tcggcaacgc accagcgagt atgcgggacc gtccgacgtt gcgtacgcca tcgaccagac
     2821 cgacccatac aaaccgacgt tcaacgtcag caacggggtg aacctggagg atccgacggc
     2881 attctcattg gacaactcca gcactgcaga cgaaagggcg gccgcccgcg cgattgcagg
     2941 gcgactcgac ctgaccgtac ctatacgggt caacggcgcc gatctcgcac tcaagtttgg
     3001 gggaaagatc cgcgacgatc ggaagaccca aagcgtggcc gacagcgact acgatgcaac
     3061 tactgcgccc gcgctaaacc tggcccaggt cgagagctcg tttttcaatc cgaactacta
     3121 tttcaactac ttcacccaca caggctacgt cctcgggccg gtggtggatt tcgacgcgaa
     3181 caatgcgatg ctcgcggccg acccgacggc gctcgagctc aacgtcaacg acacgcacgc
     3241 cgattccgac cccaacaact tcactgcgca agagcgggtc tatgcgacct acggtatggc
     3301 gacattgcgc gctggacacc tccacgttcg agccggcgtg cgcttggaag ccacccgagc
     3361 cgactacacc ggctatgtgg tgcacttcaa cgacgcgggg gactggttgg ggacttcacc
     3421 agtcagcggc agtcagagct atacagacgt cctaccgagc acagagctgc gctatgagct
     3481 ggacgacaac acgaacctgc gggccgccta cggggaggga atctcgcggc ccaactacgc
     3541 cgacctgccc cccttccggg ttcagaacga cgagaaggcc gagatcgaca tagggaatcc
     3601 gaatctgatt ccaacgcgtg ccaacaactt cgatctcttg ctcgagcatt acttcacgac
     3661 ggtcggcgtg ctctggggag gtttttttta caagaacctg aaggacccga tctacgaggc
     3721 agtcgagacc cagatcacca gtggccccga tgtcggcttc actcagtcgc aaccgatcaa
     3781 cggcccgagt gcatacgtgt ggggcatcga gctggcctgg cagcagcatc tcaccttctt
     3841 gccgggagtt ctgagcggac tcggcatcct cgcgaactat gcgtacagct actccaaggc
     3901 aaccgtgcca ggtcgcgcag accagccatc cctcgagcga caggcgcccg agacgtggaa
     3961 tctcgggctg agctatgacc gtggcgggtt cgccgggcgg attgcggcca cgcataacgg
     4021 cgccagcatc ttcgcttaca actttcagga tggcgcggat ggcggactcg ccggccccaa
     4081 cggagatacc tacctctatc cgcggacgca gttggacgct caggcaggct atcggctcag
     4141 aaacgggatg gagttctttg tccaagtgct gaacatcacc aacgaagtct tcggcttcta
     4201 caacggaagt gagcaattcc cgattcagcg agagtattac ggctggtcca cctcgctcgg
     4261 aatgcgtctg acccgttgag gacgccgctg accactacca agagaggcca tcatgcgcgg
     4321 ggcgtccatt ctgggtgtgg taacggcctt tgcagtcggt ggggcgccgg cctcccctcg
     4381 actcgagccg ccgtatcacc tgattcgtcg cgtggtcctg ggcggtcaag gcaaatggga
     4441 ttacctcacc atggatccgg ttggccgccg cgtcttcatt gcccacggca ccgaggtgtt
     4501 ggtcgtcaac gccgacagcg ggtcagtggt cggcaagatc ccggatacac cgggggtcca
     4561 tgggatcgcc ctcgcacaga gcctcggccg cgggttcatc agcaacggcg ccgattccac
     4621 cgtcacagtc ttcgatctga cgtccctcaa ggccctcgac cgtatcaccg taaccggccg
     4681 gaagccggat gccatcgtct atgactccgt gtccgggcgg gttttcacct tcaatggagg
     4741 cagcgccaac gcgaccgcga
//
LOCUS       DASRJQ010000591         2631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2351098, whole genome shotgun sequence.
ACCESSION   DASRJQ010000591 DASRJQ010000000
VERSION     DASRJQ010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2351098"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1460)
                     /locus_tag="VFB89_13410"
     CDS             complement(69..1460)
                     /locus_tag="VFB89_13410"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HZM28339.1"
                     /translation="MKNGAGPRTLWTAGGAADRRMLAYTVGDDPLWDSRLLRWDVLGS
                     LGHIEGLRAADLLSKREHARLRAALRQALKAVEAGRLRVGRGHEDVHTAVEDWLTRRL
                     PGIGERIHAGRSRNDQIACDIRLYLKATLLSLQGSALDLADALLAFASRHRTMLWPGY
                     THQRRAMPSSAALWALAFAEGLLDTTETLPALWAMLDRSPLGSAAGYGAPLPIRRETI
                     ARALGFAAPEHAVTAVQNGRGKLEAAVLFWCTQLGHELGKLAQDVILLSADEFGFLVI
                     PKELSTGSSIMPHKRNPDLFELTRARAAALDSDLAAVLHLKSKLSSGYHRDFQLLKEP
                     LFRGLERTAEVLGMMTHAIPLLQVDRARAKAAMTGGTLATDEVMRRVEQGQPFRLAYQ
                     DVAAEVANGAGLARPSAALLVSRRSSTGGLGNLGLPAVRLRLRRARRWGIRESRRFGA
                     AMARLAGRRGRLE"
     gene            complement(1457..2527)
                     /locus_tag="VFB89_13415"
     CDS             complement(1457..2527)
                     /locus_tag="VFB89_13415"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZM28340.1"
                     /translation="MPDEVELLAQLVAIPSVSGSEQGIAEFVEQTARKWRMDVVRDDT
                     SVRIEVRGFGQGQTLALVSHLDTVPPGGGWTRDPFTPGFEGGRLYGRGSTDAKAAVAA
                     MLCAARDVIERGRLDAGRLLLVFGYGEETKDTTMEEAVAGLGSVDAAVIGEPTNLDFA
                     IAQRGLMMVDLIAEGDQRHAGYAAADGEFTNAVMVLANDLLRLQSLFEDRGHPLLGLA
                     TATPTMLEAGVSRNVTPPVAKAVLDVRSTPAWTHDELATRLRESLHSQIVVTSRRLVP
                     CETPPGSRLLAAAHRARPSAREFGSPTCSDWVFLRDRDAIKCGPGTSRRSHTPDEFVD
                     LAEVSEARSFYAELARVYLAGG"
BASE COUNT          454 a          925 c          835 g          417 t
ORIGIN      
        1 ctgcgtgtgt acggcgcgag tgctgatgag ctcgagaatc cggaggtgac gcttcttccg
       61 gtcggcggtc actcgagacg tccccttctc cctgcgagcc gcgccattgc agccccgaat
      121 cgcctacttt cccggatgcc ccaacggcgt gcccggcgca agcgcaaacg caccgcaggc
      181 aggccgagat tgccaagacc gccggttgag ctgcggcggc tcaccaacag cgcggcgctc
      241 ggccgggcca ggcccgcccc gttcgcgact tcggccgcca cgtcttgata ggcaagccgg
      301 aatggctgac cctgctcgac tctccgcatc acttcgtcgg tggccagggt accaccagtc
      361 atcgcagctt tagcccgggc ccgatcgacc tggagcagcg ggatcgcatg cgtcatcatg
      421 ccgagcacct cggcagtccg ctcgagaccg cgaaacagtg gctctttgag cagctggaag
      481 tcacggtggt aaccgctcga aagcttcgac ttgagatgga gcacggccgc gagatcgcta
      541 tccagcgccg ctgcccgggc gcgagtgagc tcgaacaggt ccgggttccg cttgtgcggc
      601 atgatgctcg acccggtgct caattccttc gggatgacca gaaacccgaa ctcgtccgcg
      661 ctcagcagga tcacatcctg cgccagcttg ccgagctcat gccccaactg agtgcaccag
      721 aagagaaccg cggcctcgag cttaccgcgg ccgttctgca ccgctgtcac cgcgtgctcc
      781 ggagccgcaa aaccgagcgc ccgggcgatg gtctcacggc gaatgggcag cggcgcaccg
      841 tatcccgccg cgcttcccaa gggcgaacga tccagcatcg cccagagcgc agggagagtc
      901 tcagtggtgt cgagcagtcc ttccgcgaat gcgagcgccc acaacgccgc cgaggatggc
      961 atggcgcgac gttgatgggt gtacccgggc cacagcatag tccgatgccg ggaggcaaag
     1021 gcgagcagcg cgtccgccag atcgagcgca gagccttgga gggagagcag ggttgccttg
     1081 aggtataacc ggatgtcgca ggcaatctgg tcgttgcgag agcggcccgc gtggatccgc
     1141 tcgccgatgc ccggcagccg ccgtgtgagc caatcttcca ccgctgtgtg tacgtcttcg
     1201 tgcccgcgac cgactcggag acgcccggct tcaactgcct tcaacgcctg ccgcaacgcg
     1261 gcccggagcc gcgcgtgctc ccgctttgag agcaggtcgg ctgcccgcag tccctcgatg
     1321 tgccccaggc tgcccagcac gtcccagcgc agcagccgag agtcccagag cggatcgtca
     1381 ccgacggtgt aagcgagcat ccgccggtcc gccgcaccgc cggctgtcca gagcgtacgg
     1441 ggaccggcgc cgttcttcat ccgcccgcca ggtatacccg cgcgagctcg gcatagaagc
     1501 tccgcgcttc ggacacttcc gcgaggtcca caaattcgtc aggcgtgtgc gagcgacggc
     1561 tcgtgccagg gccgcacttg atggcatccc ggtccctcag aaatacccag tcggagcagg
     1621 tggggcttcc gaattcgcgg gcagaggggc gggccctgtg agctgccgcc agcagtcgcg
     1681 agcccggagg cgtctcacac ggcaccaacc ggcgtgaggt gacgacgatc tgcgaatgaa
     1741 gcgactcacg cagccgggtg gccagctcgt cgtgggtcca ggctggagta ctgcgcacgt
     1801 cgagcactgc cttggcaacc ggcggcgtga cgtttcgact cacgccggcc tcgagcatcg
     1861 tgggtgtagc ggtggcgagg ccgaggagag gatgaccccg atcctcgaac agcgattgca
     1921 accgcaggag atcgttcgcg agcaccatca ccgcgttggt gaactctccg tcggcggcgg
     1981 catagcccgc atgccgttga tcgccttccg caatcaggtc caccatcatg aggccccgtt
     2041 gcgcgatggc gaagtccaga ttggtgggct cgccgatcac ggcggcatcg accgacccca
     2101 gcccggctac ggcttcctcc atggtcgtgt ctttggtttc ctcgccatag ccgaacacca
     2161 gcagcaggcg tccggcatcc agccggccgc gttcgatcac atcccgcgcg gcgcagagca
     2221 tcgctgccac cgccgccttg gcgtcggtcg agccgcggcc atagagccga ccgccctcga
     2281 agcctggggt gaatgggtcg cgggtccacc ctccccccgg cggcacggta tcaaggtgtg
     2341 aaaccagggc caaagtctgg ccctgcccga acccacgcac ctcgatgcgt accgacgtgt
     2401 cgtcgcggac gacgtccatc ctccatttcc gagcggtctg ctcgacgaac tccgcgatcc
     2461 cctgctcgct cccgctgacc gagggaatcg ccaccagctg cgcgagcagc tccacctcat
     2521 ccggcaagat ctactccgat gtaggtcgcc ttctccttgc gccccacgat catggtcccg
     2581 cagccggcac cagtgaacac ctcgagcaaa agcgcatccg gagcgcggcc g
//
LOCUS       DASRJQ010000592         4138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2632561, whole genome shotgun sequence.
ACCESSION   DASRJQ010000592 DASRJQ010000000
VERSION     DASRJQ010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2632561"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1232
                     /locus_tag="VFB89_13420"
     CDS             <1..1232
                     /locus_tag="VFB89_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28341.1"
                     /translation="AQFRGRRPAMLMLALSDERGREGGKNDGRAPKSAREQNGAPRLS
                     GIQHTIDAGIGGRHSPASYWSVGPRVSSVELMSLSPRLALGLELCRVWALALAFGIPS
                     ARAQDVNLPNQHFEVTPEPGRSHVGNVVVLRFRVRLDERDLLYDTVPRPVAELAPGVR
                     ILSVEKLLRGSDRIYTGKAQVAFYRTGRQAAPIFGLPFMRAVKGVTRATMTSDSAFVE
                     IDPVAPAGNPSLKDIKDIERQRGPDPRVVAGVLGAALVALVGVLLGRHRRAGPLKSLE
                     RADTLPADAGPYQAALARLDAIEESGWPAGGEIDRHYAAVADVLRRYLEEAHEVPALE
                     RTTAELLFLLPRPLAGDDLRRHCEQLLAEADLVKFARYRPDIGEAQAVVRAARNLLAE
                     WHRAAAGPVPATSYALR"
     gene            1219..2211
                     /locus_tag="VFB89_13425"
     CDS             1219..2211
                     /locus_tag="VFB89_13425"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZM28342.1"
                     /translation="MRFAEPWFLALLACPLWWGWTLWRARKQPPAYLGFSALTLLGAV
                     PRSARVRWHRIAPWIPLLGLTLLILALARPQSARHLEDIKVRSRNLMLVLDISSSMKA
                     SDFKPGNRLEVARKVLGDFARQRDGDLLGLVVFAGRAFLQAPLTPDAGLVESMLAKVD
                     IGQLPDGTAIGTALALALAQLKDLPANASTIVLVTDGANNTGKPTPFVAAEAARALGI
                     RIHAIGVSSADTTGHNRRFIWRWGGRQADRLTSSDEAILRRVADRSGGRYFRATDPEM
                     LASIMREIDPLERVEVRIGETRDWRELFPFLLVPGLLLLSLELGLGLGRLRTVP"
     gene            2219..3958
                     /locus_tag="VFB89_13430"
     CDS             2219..3958
                     /locus_tag="VFB89_13430"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZM28343.1"
                     /translation="MTFAHPALLLLLLLLPAGVLLVRRSEKRRQAALARFGNPDLLAP
                     LSAFPDGRRREARNWLRLAALACLIVAAARPQAGEQPAVIRRAGRDVLVLLDLSRSMN
                     VSDAGGTRLGRAKTLLAELAAARPLDRLGLIVFGGSAFLQLPLTTDHDAFRRFVEAAA
                     TENVGDPSTDVSAALAAAATAFEHAGEPGSRAVVLVSDGERTEGALDRPLARLRREEV
                     PVFAIGVGSVAGGNVPADSSEAPEPYHRDNIGRIVVSRLDEADLRKIAEQTGGAYARW
                     DDAAAIQLVQQAVERLEARPITAHKARRPAERFQWPLGAAVLLLMVEWLAPLRVRRPA
                     ALPSRRPELTDAAVAVGLALLIVGLFGCSPTSWQARRAERRYDAGDWPGAFAEWSRAL
                     EASSNPVFGYDAGNALYRQKQYKDAATRWRAAATDQRLRQRSAYNQGNAAVRAAEDAT
                     GAEKDSLLDGAVAAFEEAVRLGPADQDAKWNLEIALRRRGDRPEGSSSGRSRRGDYGQ
                     GNQNVPGMEGKPEAATGAMAGGGFGSGADGESVKELDPTQARQLLDAVQREQLASHLG
                     RRTSHPSRGEKDW"
BASE COUNT          654 a         1366 c         1410 g          708 t
ORIGIN      
        1 tggcccagtt tcggggtcgg cgccccgcca tgctgatgct cgccttgagc gacgagcgag
       61 ggcgcgaagg cggcaagaac gacggccgtg cgccaaagag cgcgagggaa cagaatggtg
      121 cgcctcgtct aagcggtatc cagcatacga tagatgccgg gatcggcggt cgccatagtc
      181 ctgcgtccta ctggtccgta gggccacggg tgtctagtgt agaactgatg tccctctctc
      241 cgcgcctggc cctaggtctc gaactgtgca gggtgtgggc actagcgctg gctttcggaa
      301 ttccgtccgc cagagcccag gacgtcaatc tccccaacca gcatttcgag gtcacgcccg
      361 agcccggccg ctcgcatgtc ggcaatgtgg tcgtgcttcg gtttcgcgta cgactggacg
      421 agcgcgacct cctctatgac actgtgccac gacctgtggc agagctggca ccgggggtcc
      481 ggattctgtc ggtcgaaaag ctactgcggg gctcggaccg gatctatacg gggaaggccc
      541 aggtcgcatt ctatcggacc gggcgccagg cggcgcccat cttcgggctt ccgttcatgc
      601 gcgcggtgaa gggtgtaact cgcgcgacta tgaccagcga cagcgcattc gtggagatcg
      661 acccggtcgc gccggcagga aatccgtcgc tcaaggacat caaggatatc gagcgacagc
      721 gcggccccga tccgcgtgtc gttgccggtg tgctcggcgc cgcactggtg gcgctggtgg
      781 gcgttctcct gggtcgacat cgccgggccg ggccgctcaa atcactcgaa cgggcggaca
      841 cactccccgc agacgccggc ccgtatcagg cagccctcgc gcggctggat gcaatcgagg
      901 agagtggttg gcccgcgggt ggggagatag accggcatta cgcggcggtg gccgacgtgc
      961 tgcggcgcta tctggaggag gctcacgagg tgcccgcact cgagcgcacc acagccgagc
     1021 tgctcttctt gctgccgcgg ccgctcgcag gggacgactt gcggcggcat tgtgaacagc
     1081 tgctggccga agccgatctg gtaaagttcg cccggtaccg acccgatatc ggtgaagctc
     1141 aggccgtggt gcgagccgcc cgcaacctgc tcgccgaatg gcatcgcgcc gcagcggggc
     1201 cggtcccggc aaccagctat gcgcttcgct gagccctggt tcctcgctct gctcgcgtgc
     1261 cccctctggt ggggctggac gctgtggcgc gcccgtaagc agccaccggc atatctcgga
     1321 ttctccgcgc tcacgctgct cggcgcggtg cctcgaagcg cgcgcgtccg ctggcatcgc
     1381 atcgcgccgt ggattcccct actcggcctg accttgctga ttctggctct ggcccgcccg
     1441 cagagcgcac gacacctcga ggacatcaag gtgcgatcgc gcaacctcat gctcgtcctc
     1501 gacatctcga gcagcatgaa agcgagcgac ttcaagccgg ggaaccggct cgaggtcgcc
     1561 cgaaaggtcc tgggcgattt cgcccgccag cgcgacggcg acctgttggg actcgtggtc
     1621 ttcgccgggc gcgcgttcct ccaggcaccg ctcacgccgg acgcaggact ggtcgagtcg
     1681 atgctggcga aggtggacat cggtcagctc cccgacggca ccgccatcgg gacggcactg
     1741 gcccttgccc tggctcagct caaggatctc cccgccaacg ccagcaccat cgtgctggtc
     1801 acggatggcg ccaacaacac cggaaagccg accccattcg tggcggcgga ggcagcccgc
     1861 gcccttggca tccgcatcca tgcgatcggc gtgagttcgg ccgacaccac cgggcacaat
     1921 cgtcggttca tctggcgctg gggcggtaga caggccgacc gcctgaccag ctccgacgag
     1981 gcgattctcc ggcgtgtcgc cgaccggagc ggcggccgct atttccgcgc gaccgacccg
     2041 gagatgcttg cgagcatcat gcgagagatc gatccgctgg agcgggtgga ggtgcgcatc
     2101 ggggagactc gcgattggcg ggagctcttt ccattcctgc tcgtgcccgg actcctgttg
     2161 ttgtccctcg agctcgggct tggtctcggc cggcttcgta ccgtgccgtg aggcgccgat
     2221 gacgttcgcg catcccgccc tgctcctcct gttgctcttg ctccccgcgg gcgtgctcct
     2281 ggtccgacgc tccgaaaagc gtcgccaggc agccctcgcc cgattcggca acccggatct
     2341 cctcgcgccc ctctcggcgt tcccggacgg gcggcggcgc gaggctcgca actggctccg
     2401 gttggcggcg cttgcctgtc tgattgttgc cgctgcacgg cctcaggcgg gtgagcagcc
     2461 cgcggtgatt cggcgcgcag ggcgcgacgt gctcgttctg ctcgatcttt cccgctccat
     2521 gaacgtctcc gatgcgggcg gtacccggct cggccgggcc aagacactcc tggccgagct
     2581 cgccgcagcg cgcccgctcg atcgcctggg actgatcgtg tttggtggaa gcgccttcct
     2641 tcaattgccg ctcaccaccg accacgacgc gtttcgccgg tttgtcgagg cggccgccac
     2701 cgagaacgtg ggtgatccgt cgaccgatgt ttcggcggcg cttgctgcgg cagccacggc
     2761 tttcgagcac gcaggggagc cggggtcgcg cgctgtagtg ctcgtctccg acggcgaacg
     2821 aaccgaaggc gctctcgacc ggccgctggc acggttgcgt cgggaagagg taccggtgtt
     2881 tgccatcgga gtcggcagcg tggccggagg gaatgtgccc gccgacagca gcgaggcgcc
     2941 ggaaccgtac caccgcgaca acatcgggcg cattgtcgtt tcgcgcctgg acgaagccga
     3001 cttgcgaaag atcgccgagc aaaccggtgg cgcctacgca cgctgggacg acgcagcggc
     3061 aattcagctg gttcagcagg ctgtcgagcg actggaagcc cgtcccataa cggcccacaa
     3121 ggctcggcgc cctgcggagc gctttcaatg gccgctcggc gcggcggtgc tgctcttgat
     3181 ggttgaatgg ctggcaccgc tccgcgtccg acgccccgcg gcgctgccgt cgcgccggcc
     3241 tgaactgacg gacgcggctg tcgccgtagg gctcgcgttg ctgatcgtgg ggctgttcgg
     3301 ctgcagtccc acgagctggc aagctcgacg tgcggagcgg cgctacgatg cgggagactg
     3361 gcccggtgcg tttgccgagt ggagtcgcgc gctcgaagca agcagtaatc cggtgttcgg
     3421 gtacgatgct ggcaatgcgt tgtaccggca gaagcaatac aaggacgccg cgacccgttg
     3481 gcgcgcagct gcgaccgacc agagactacg acagcgaagc gcgtacaacc aggggaatgc
     3541 tgccgtacgg gcggccgaag acgcgacggg cgccgagaag gattcgttgc tcgacggtgc
     3601 cgtcgccgcg ttcgaggaag ccgtccgcct cggcccggcc gatcaagacg cgaaatggaa
     3661 tctggagatc gctctccgcc gacgaggtga ccgacccgaa ggcagctcct ccggccgatc
     3721 gcgccgcggt gactatggcc aaggcaacca gaatgtgccg ggaatggagg ggaagcccga
     3781 agctgctacg ggtgccatgg ccggcggagg atttggctcg ggggctgatg gtgagtcggt
     3841 caaggagctg gatccgaccc aggcccggca attgctggat gccgtgcagc gtgaacaact
     3901 agccagccat ctcggccgcc ggacgagcca tccctcgcgt ggtgagaagg attggtaggc
     3961 ggaacgccag agttgcaaag gaaagtgcga tggtctcccc gagtttcggc caagccaggt
     4021 atcgttcagt ggcagcagag gttctgctgc ctgccggctg gattaccgga accttcgtag
     4081 tacccgaaga ccagagcttc ctcgacttcc tggcccactc cggcgccttt ctcaagct
//
LOCUS       DASRJQ010000593         5782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_377532, whole genome shotgun sequence.
ACCESSION   DASRJQ010000593 DASRJQ010000000
VERSION     DASRJQ010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5782
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377532"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..1632)
                     /locus_tag="VFB89_13435"
     CDS             complement(163..1632)
                     /locus_tag="VFB89_13435"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZM28344.1"
                     /translation="MVAKNPEIGQGVKTALPIIVAEELDVEWSRVRVVQGDLNPQFGE
                     QFAGGSTALKAAFRRHIDQPGTVLRADGDPDGSLRSAAKVVTADYEVPFLAHATMEPV
                     NCTASVTGGGTECEIWGPMQDPGGAQDLVAQVSGIARERIRIHLTPSGGGFGRRLLSD
                     YVAEAALVSKAVGVPVQVVWTREDDLAHDYYRPAGLHRLSAGLDPQGNLVTWTHRLVN
                     TSRYAFAGRTDGPEKSEMYPDDFPAGSIPNLRLDYAAVPCTVPVGAWRSTLHSSNAFA
                     VESFVDEVALVAQRDPLAFRLALLEPGRILKYRGHGGPEFNTGRLAAVLRTVAEKSGW
                     GTPAPSGSARGIAAHFTFGSYAADVVELSGNSAGGVRVQRVVCAVDCGLVVNRSGAES
                     QVEGGIMDALGAALHGEITVKAGQVEQRNFDRYPLLRMNEAPTVEVHFVNGAANLTGL
                     GEPPVPPVAPAVANAWFALTSQRVRRLPLVPAVRAGRSS"
     gene            complement(1858..2322)
                     /locus_tag="VFB89_13440"
     CDS             complement(1858..2322)
                     /locus_tag="VFB89_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZM28345.1"
                     /translation="MPLTITVNGGRRTIRVAPDTPLLWVLRDTLGLTGTKYGCGIGQC
                     GACTVHLGGVATRSCLIPVGKVGSKPVITIEGLSADGLHPVPQAWLAEDVPQCGYCQA
                     GQVMTAAALLRSNPHPSDDEIEAAMQGNLCRCGTYQRIRRAVRRAAGQERAG"
     gene            2511..3062
                     /locus_tag="VFB89_13445"
     CDS             2511..3062
                     /locus_tag="VFB89_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28346.1"
                     /translation="MRIPTAIRVFGPAVPSYGELAAEAWALVFQADFRQTGLADRIEY
                     AVRARRLSLAAQWERLQDINFFNYYRYYILPRLAPALAASLLALYLWDTASHTGPELW
                     AIIQLMLLAVATASLWALLHWPQPLRDLVFAARYRAARLDRERQLLSPDGWNGWRAER
                     RADGTYIMVPVDRHGTSRRPQPS"
     gene            complement(3050..5515)
                     /locus_tag="VFB89_13450"
     CDS             complement(3050..5515)
                     /locus_tag="VFB89_13450"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM28347.1"
                     /translation="MNSAAQLQSAVATSYQIQRELGRGGMAVVYLAHDLRHDRLVAIK
                     VLEPRLAVLLGRERFLREIRLAAKLQHPHIVPVHDSGAYECGPESVGLYYVMPYVEGE
                     SLRARLAREGRLPIPDAVRVAREVADALACAHAHGVLHRDIKPENILLSQGHAQVADF
                     GIAKPLAPAEGGSGAQTSVTETGLVIGTAAYMSPEQASGERALDARTDIYSLGCVLYE
                     MLIGHPPFSGATPQAALVSRLTSDPPTARSLRPELPTALDAALSRALARDVSARFRTA
                     GQLEQALSGAMDQARGSSTAPVGSKVWRAGPAGTGRRRVWLGVMAAIGVLVLAAGVAA
                     LWLSRMRTGSPASTVGPVALAVLPFHMLGGSGQTSPLAVGVPDAIISRLAEARRLRLR
                     PTTAILRYADRDVDLSSVGRELAVDYLLTGTVQDAGDRLRVSVQLVRAGDGAPLWGTH
                     YDLGRQDLLTLQDSIAERVSSVLAVRMTAEEQERVYRRYTSNGAAYEAFLRGRAELAR
                     LSEPGARAAVSAFEQSLAFDSNYALAHAGLAMASADMHLRFATGAEVNEWGERARREA
                     ARALALDSNVAEAHLAQAAVYRKSEFDWEATIRESRRALALNPSLDLPHYFIAGAYYH
                     LGLLERAEAELRDAASADPTNHNEQLRTRGVLSFLGGDFNNAVRLFEQQRRESGSTVS
                     DSYLTQAYWFVGDTAHALALIDSLIRSPSAPAAARARASLASFLAARDSGRAQALLDT
                     LERAAYMDHHVAYSMAAAYAQLGRLDKARSWLRRSVETGFACYPWLIRDPLIAPLRSD
                     PGSREMFDDFRKRWEDARTRYEG"
BASE COUNT         1012 a         1975 c         1821 g          974 t
ORIGIN      
        1 aggctgctgc tgcggcagtt cagcgtgcag ccaagactct gcgcgatctg cgggtcgccg
       61 aggtctccga gctcgacatt cagctcaagg acggaaaggt ccagtcctat cgagcgaagg
      121 tgaaggtctc gttcaagtac gaaggaacgt gatcgtccgc gatcaggaag agcgacccgc
      181 gcgcacggcg ggtacgagag gaagccgccg aactcgctga ctggtcagcg caaaccacgc
      241 gttcgccacc gccggagcga ccggggggac cggcggttcc cccagcccgg tgagattcgc
      301 tgcgccgttg acgaagtgga cctccaccgt cggcgcctcg ttcatacgta gcagcgggta
      361 gcgatcgaag ttgcgctgct ccacctgccc ggccttgacc gtgatctcgc cgtgcaacgc
      421 ggcgcccagc gcatccatga tcccgccctc gacctgcgat tccgcgcccg accgattcac
      481 caccagcccg cagtccactg cacagaccac tcgctgcacg cgcacaccac cggcactgtt
      541 ccccgacagc tctaccacgt cggcggcata actgccgaag gtgaagtgcg ccgcgattcc
      601 ccgcgcgctc cctgagggtg ccggcgtgcc ccaccctgat ttctccgcga ccgtccgcag
      661 cacggccgca aggcgtcctg tgttgaattc aggtccgccg tggcctcgat acttgagaat
      721 ccggccgggc tcgagcaacg ccaaccgaaa cgcgagtggg tcacgttgcg cgaccagcgc
      781 gacctcgtcc acgaagcttt ccacggcgaa ggcgttcgac gaatgcagcg tggagcgcca
      841 tgctcccacg ggcaccgtgc acggcacggc ggcatagtcc aggcgcagat tcgggatgct
      901 tcccgcgggg aaatcgtcgg ggtacatctc cgacttttcc ggcccatcgg tgcgaccggc
      961 gaaggcgtac cgtgaggtgt tgaccaggcg atgtgtccag gtgacgaggt tcccctgcgg
     1021 atcgagcccc gccgagagcc ggtggagacc cgcgggccgg tagtagtcgt gcgcaaggtc
     1081 atcctcgcga gtccacacga cctgaaccgg tacgcccaca gccttcgaga ccagagctgc
     1141 ctccgccacg tagtccgata gcagccgtcg tccaaaccca ccacccgaag gagtgagatg
     1201 aatccggatt cgctcccgcg cgataccgga cacctgcgca acgaggtcct gcgcgccgcc
     1261 agggtcctgc atgggacccc aaatctcgca ctcggtgccg ccgccggtca cgctcgccgt
     1321 acagttgacc ggctccatgg tcgcgtgggc cagaaacgga acctcgtagt cggcagtcac
     1381 cactttcgcc gcggagcgaa gcgacccgtc gggatcgccg tcggcccgaa ggaccgtgcc
     1441 gggctggtcg atgtgcctgc ggaacgccgc tttcagagcg gtgctgcccc cggcaaactg
     1501 ctcgccgaac tgagggttca gatcaccttg cacgacccga acgcgggacc actcaacgtc
     1561 cagctcttcc gccacgatta tgggcagcgc tgtcttcaca ccctgaccga tctccgggtt
     1621 cttggccacc atggttcacg ctgccgtctg ggtcgatttg gatgaatggc cccaaccctg
     1681 cggggggtgc ggctccggaa ccaccgtctg tcgccgcacc gaggagcgcc cgtctcgagc
     1741 cgcccgtagg cagaaccgct ccgacgatca atccaccggc ggccgagccg ccgatccaga
     1801 gaaaggctcg ccgactgatg ctcgcgttca tgcgtcgctc cggatctgtc gacgctgtca
     1861 tccagcgcgc tcttgaccgg ccgcccgacg gactgcccgg cggatccgct gataggtccc
     1921 gcaacggcag agattccctt gcatcgccgc ctcgatctcg tcatcgctcg gatgtgggtt
     1981 ggagcggagc agcgccgccg ccgtcatcac ttgtcccgcc tggcaatagc cgcattgagg
     2041 cacgtcctcg gccagccagg cctgcggcac tggatgcagc ccgtcggcag aaaggccttc
     2101 gatggtgatc accggcttgg atccgacctt gccgaccgga atcaggcagg agcgggtcgc
     2161 gactccgccg agatggaccg tgcaggctcc gcattggccg atgccgcagc catacttggt
     2221 gccggtgaga cccagcgtgt cgcggaggac ccaaaggagt ggcgtgtccg gcgcaacccg
     2281 gatcgtgcga cggcctccat tgaccgtgat cgtgaggggc attgcgaccg gctacagtgg
     2341 ggacctaaac ccgacgatac cctgaatcaa atgtttcagc cttgcccctt gcgcggatgc
     2401 tgccccggta cgcatcgtac cgtaccctga tatagatcgc gcctgccacg cttcccctgc
     2461 cggggggatc tgcgagctgg agcggcaggc gtgtcccagg agcacagaga ttgcgaatcc
     2521 ctactgccat ccgagttttc gggccggccg tgccttcgta cggcgagctg gccgctgaag
     2581 cctgggcact cgtctttcaa gctgatttcc gccagaccgg tctggccgat cgcattgaat
     2641 acgcggtccg cgcgcggcgt ctctctttgg cagcgcagtg ggaaaggctg caggacatca
     2701 atttcttcaa ttattaccgc tactacattc tgccgcgctt ggctccggca ctcgccgcgt
     2761 ccttgctggc attgtacctc tgggacacgg cgagccatac cgggccggag ttgtgggcca
     2821 taattcagct catgctcctg gcggtggcga cagccagtct ttgggcgctg ttgcattggc
     2881 cgcagccgct gcgggatctg gtttttgccg cgcgctaccg cgccgcccgc ctcgaccggg
     2941 aacgccagtt gttgtcgccg gacggttgga acggttggcg ggccgagcgc cgggctgatg
     3001 gcacctacat catggtgcca gtggaccgtc atggcaccag tcgccggcct cagccttcat
     3061 aacgcgtccg cgcgtcttcc cacctcttcc tgaagtcatc gaacatctcg cgcgatcccg
     3121 gatcgctccg gagcggcgcg ataagcggat cgcgtatcag ccagggataa caggcaaacc
     3181 cggtctcgac gctgcgacga agccagctgc gggccttgtc taatcgaccc aactgagcgt
     3241 acgccgcagc catgctgtac gcaacgtggt gatccatgta agccgccctt tcaagcgtat
     3301 caagcagcgc ctgggctcgc ccactgtctc gtgctgctag aaagctcgcc agactggcgc
     3361 gagcgcgagc cgccgccggt gccgagggtg atcggatcaa gctgtcgatc agtgccagcg
     3421 catgggccgt gtcgccgacg aaccagtatg cctgggtcag gtaggagtca gagacggtgc
     3481 tcccgctttc ccgccgctgc tgttcgaaca gacgcacggc attgttgaag tcaccaccaa
     3541 gaaaagaaag cacgccgcga gtgcgcagtt gctcattgtg gttggtggga tcggctgatg
     3601 ccgcatcgcg cagctctgcc tcggctcgct cgagcaggcc gaggtgatag tacgctcccg
     3661 caatgaagta gtgaggaaga tcgaggcttg gattcagggc tagggcgcga cggctctccc
     3721 ggatagtcgc ttcccaatcg aactcggatt tacgataaac cgccgcctgc gcgaggtgtg
     3781 cctccgccac attggagtcg agggccagcg cccgagctgc ctcacggcgc gctcgctcgc
     3841 cccattcatt cacctcggca ccggtcgcga accgcagatg catgtcggcc gacgccatgg
     3901 caagcccggc atgcgccaga gcatagttgc tgtcgaaggc caacgactgc tcgaaagcag
     3961 agaccgccgc gcgagcaccg ggctccgaca ggcgggccaa ctcggcacgg ccacgaagga
     4021 acgcctcgta cgccgcaccg ttgctcgtgt agcggcgata cacccgctcc tgctcctcgg
     4081 cagtcatgcg cacagcgagg acgctactta cccgctctgc gatcgagtcc tgcaaggtca
     4141 gcaagtcctg tcgccctaag tcgtagtgcg tgccccagag cggggcgccg tcgcccgctc
     4201 gcaccagctg cacgctcacc cgcaggcgat cgccggcgtc ctgcacggtc ccggtcaaca
     4261 gatagtccac cgccagctca cgtccgacgg acgacagatc gacgtctcga tcagcatacc
     4321 ggaggatggc agtggtgggt cgcaatcgca gcctgcgcgc ctcggcaagc cggctgatga
     4381 tcgcgtccgg aacgccgaca gcgaggggag acgtctggcc cgatccgccc aacatatgga
     4441 acggcagcac cgctaaggcg acggggccga ctgtgctggc aggagaccca gtccgcattc
     4501 ggctgagcca tagagcggcg actccggccg caagaaccag gacgccgatc gccgccatga
     4561 cgccaagcca gactcgcctg cggccggtcc cggccggccc ggcacgccac accttcgacc
     4621 ccactggcgc ggtcgagctg ccgcgagcct ggtccattgc acccgacaac gcttgctcca
     4681 gttggccggc agttcggaat cgtgcgctga cgtctcgggc cagcgcacgg ctcaacgcgg
     4741 catcgagcgc cgtcggcagt tctggtctga gggaacgcgc cgtcggcggg tcgctggtca
     4801 gtcgtgacac caatgcggct tgcggcgtag cgccgctgaa tggcggatgt ccgatgagca
     4861 tttcgtagag gacgcagccg agcgagtaga tgtcggtccg cgcgtccagc gcccgttcgc
     4921 cactggcctg ttcggggctc atgtacgcgg ctgtcccgat caccaggccc gtctccgtga
     4981 cgctggtctg cgcccctgat ccgccttcgg ctggagcaag cggcttcgcg attccgaagt
     5041 cggcaacctg ggcgtggccc tgcgagagca gaatattttc cggcttgatg tcacggtgca
     5101 gcacgccgtg ggcatgcgcg cacgccaggg catctgccac ctcacgtgcc acgcgaacag
     5161 catcgggaat cggaagtcgt ccctcgcgag caagcctcgc tctcagcgac tctccctcga
     5221 catacggcat cacgtagtag aggccgaccg actccggacc acactcgtag gcgccggagt
     5281 cgtgcactgg cacgatatgc ggatgctgca gcttcgccgc gagccggatc tcccgcagaa
     5341 accgctcgcg tccgagcaag acagccaggc ggggttcgag aactttgatt gccacgaggc
     5401 ggtcgtggcg aagatcgtgc gctaggtaga cgacggccat gccgccgcgt ccgagctcgc
     5461 gctggatctg gtaggacgtc gctacggcag actgcagctg cgcggctgag ttcatagacg
     5521 ccgcggaagt tgatctccaa gaataggccc tctgcggatg accgaccagc tgttggggcg
     5581 gagcatctgg cgcgacacgg ccgggggccg cattacgtaa gggcggaaca gccccaccct
     5641 acaccaggaa ccgtcgatgc gcccgaaacg ccgtgcactt cctctgctcc tcgtcatcgc
     5701 cggagtggct ctggtccctc gaggcgtgat ggcccagacc tctgcccccc ctcttgccgc
     5761 cacgatggac tccattccgg aa
//
LOCUS       DASRJQ010000594         3912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2102985, whole genome shotgun sequence.
ACCESSION   DASRJQ010000594 DASRJQ010000000
VERSION     DASRJQ010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2102985"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VFB89_13455"
     CDS             <1..224
                     /locus_tag="VFB89_13455"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HZM28348.1"
                     /translation="NPIIIPQPQSAILGIGKVEPRPVVRERDGRETIEVRPMCYVTLT
                     IDHRVLDGFQANAFLTRWVKEIEGWPKEP"
     gene            complement(211..717)
                     /gene="paaD"
                     /locus_tag="VFB89_13460"
     CDS             complement(211..717)
                     /gene="paaD"
                     /locus_tag="VFB89_13460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HZM28349.1"
                     /translation="MNATDAQAAALLEVLHGVPDPEVPALDIVELGIVRKVERRPDGV
                     VVVEVTPTYSGCPAMRTIEEEIVVALRANGYERVELRTVYSPAWTTDWLSDEAKAKLK
                     AYGIAPPEPAVAEQILVPLTATIRRAACPYCGSSNTERRSEFGSTACKAIHYCHHCRQ
                     PFEEFKAL"
     gene            complement(714..1460)
                     /gene="paaC"
                     /locus_tag="VFB89_13465"
     CDS             complement(714..1460)
                     /gene="paaC"
                     /locus_tag="VFB89_13465"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HZM28350.1"
                     /translation="MNPLFEYLLRLGDDRLVLGHRLSEWCGHGPALEEDIALANVALD
                     LIGQASHFLRLAGTVEGRGRDEDALAYLRDAIDYRNVKLVELPNGDFGRTIVRSFLFD
                     AWSALSLEKLVHSSHAELAGIAAKAHKEARYHVRHSGEWVIRLGDGTDESHRRTQNAL
                     DDLWPYIGELFASDEVDKAHANGIAPDPPSLEPAWREVVSDVISRATLTLPLDGYMHS
                     GARQGRHTEHLGHLLAEMQILARSHPGAKW"
     gene            complement(1468..1782)
                     /gene="paaB"
                     /locus_tag="VFB89_13470"
     CDS             complement(1468..1782)
                     /gene="paaB"
                     /locus_tag="VFB89_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016995969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HZM28351.1"
                     /translation="MAASTDQADGASGEGPLWEVFTQEKQGAPHEHAGSVHAPDAEMA
                     LQNARDVYARRGDIVSLWVVPSSAIIASSPGESGPFFDPADDKPYRHPQFYKTPRGVR
                     VY"
     gene            complement(1794..2759)
                     /gene="paaA"
                     /locus_tag="VFB89_13475"
     CDS             complement(1794..2759)
                     /gene="paaA"
                     /locus_tag="VFB89_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HZM28352.1"
                     /translation="MAATPTREDNASNAAFEARIAAGETIEPKDWMPERYRQQLMRMM
                     SQHAHSEIVGMLPEGNWITRAPSLRRKMCLLAKVQDEAGHGLYIYCATETLGVDRAQL
                     IDQLLTGKAKYSSIFNYPTLTWADIGVIGWFVDGAAIVNQTVLAKASYGPYARAMVRI
                     CKEENFHKRQGFEIVATMASGTPGQHAMVQDAVNRWWWPTLMMFGPSDANSPNSAELL
                     RWRVKRFSNDELRQRFVDLTVAQAQAVKLTLPDPKLRYNAETKHWEFGEIDWAEFKQV
                     IGGNGPCNRERLAARRAAHDNGAWVRHAAAAYAEKQRRAGAASAA"
     gene            2871..3644
                     /locus_tag="VFB89_13480"
     CDS             2871..3644
                     /locus_tag="VFB89_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZM28353.1"
                     /translation="MTRQSAQDQGSVASHVVDGVATVIFGHPKGNSLPGKLLADLAAS
                     IGDLGADDNVNVIALRSDGTGAFCAGASFAELAAISDVEGGTRFFMGFANLINAMREC
                     PKLIVTRVHGRAVGGGVGIVAASDYALATTGAQVKLSELMVGIGPFVVGPVIERKIGA
                     ANYAALGIDAASWRSAEWAYERGLFAELHANVADLDTRIAALTSQLAASNPAAMAELK
                     KVLWQGCDHWRELLPARARLSGTLVVSAPARQAIQRFSS"
BASE COUNT          677 a         1252 c         1243 g          740 t
ORIGIN      
        1 cgaatccgat catcattcct cagccccagt ccgccattct gggcatcggc aaagtggaac
       61 cccggccggt ggtccgggaa cgcgatgggc gagagacgat cgaggtgcgg ccgatgtgct
      121 acgtcaccct caccatcgac catcgcgtgt tggacggctt ccaggcgaat gcgttcttga
      181 cccggtgggt aaaggagatc gaaggctggc ctaaagagcc ttgaactctt cgaacggctg
      241 acggcagtgg tgacagtagt ggatggcttt gcaagcagtc gacccgaact cacttctccg
      301 ctcggtgttg ctcgagccgc aataggggca ggccgcgcga cgaatcgtcg cggtcaaggg
      361 caccagaatc tgctcggcta cagccggttc cggtggcgca atcccgtagg ccttgagctt
      421 tgctttcgcc tcgtcactga gccagtcggt ggtccaggcc ggcgagtaca ccgtacgtag
      481 ctcgacccgc tcgtagccgt tggcgcgcag cgcgacgacg atttcttctt cgatcgtccg
      541 catggccggg cagccggaat aggtcggcgt gacctctacg acgacaactc catccgggcg
      601 tcgctcgacc tttcgcacaa tccccagttc cacgatgtcc agcgccggga cttccgggtc
      661 cggcacgccg tgcaggactt cgagcagggc tgctgcttgc gcgtcggtgg cgttcaccac
      721 ttcgctcccg gatgcgagcg ggccaggatc tgcatttcgg cgaggagatg gcccaggtgc
      781 tcggtgtggc ggccttggcg ggcgccggaa tgcatgtagc catcgagcgg caaggtgagc
      841 gtggcgcgag agatcacgtc ggagaccact tcgcgccagg ccggttcgag gctcggtgga
      901 tccggggcga tgccattcgc atgagccttg tccacctcat cggacgcgaa cagctcaccg
      961 atgtagggcc agagatcgtc cagcgcgttc tgggttcgcc ggtggctctc gtcggttccg
     1021 tcgcccaagc ggatcaccca ttcgccgcta tgccgaacgt gataacgcgc ttccttgtga
     1081 gctttggccg cgatcccggc cagctcagcg tggctcgagt gcaccagctt ctcgagcgac
     1141 aacgcactcc aggcgtcgaa cagaaatgag cggacgatcg tgcggccgaa gtcgccgttc
     1201 ggaagctcga ccagcttgac gttgcgatag tcgatggcgt cgcgcagata agcgagcgca
     1261 tcctcgtctc gtccacgacc ctcgactgtt cccgcaagtc gcagaaagtg cgatgcctgg
     1321 ccgatcagat cgagcgccac gttggccagc gcaatgtcct cttcgagcgc gggcccgtgc
     1381 ccgcaccact ccgagagccg atgacccagg accaggcggt cgtcacccag gcgcagcagg
     1441 tattcgaaca gcggattcac cgaaccctca gtagacgcgg acgccgcggg gggtcttgta
     1501 gaactgggga tggcggtacg gcttgtcatc ggcgggatcg aagaaggggc cgctctctcc
     1561 cggactcgat gcgatgatgg ccgagctggg aacgacccag aggcttacga tgtcgccccg
     1621 acgggcatac acgtcgcgag cgttttgcag ggccatctcc gcatcgggtg cgtgtacgct
     1681 gcccgcatgc tcgtgtggag cgccctgctt ctcctgagtg aacacctccc atagcggccc
     1741 ctcgccgctc gcgccatccg cctgatcggt gcttgccgcc atttgccggt ggctcaggcc
     1801 gcgctggccg cgccggcgcg acgctgcttt tcggcgtacg ccgccgcggc gtggcgcacc
     1861 caggcgccgt tgtcgtgcgc cgcccgtcga gccgccaggc gctcccgatt gcagggaccg
     1921 tttccgccga tgacctgctt gaactccgcc cagtcgatct caccgaattc ccagtgcttg
     1981 gtttcggcgt tgtagcggag cttggggtct ggcagcgtga gcttgacggc ctgggcctgc
     2041 gccaccgtca aatcgacgaa gcgctggcgc agctcgtcgt tgctgaatcg cttgacccgc
     2101 cagcgaagca gctcggcgct gttgggagag ttggcgtcgc tcgggccgaa catcatcagc
     2161 gtcggccacc accagcggtt gaccgcatcc tgcaccatgg cgtgctggcc cggtgtgccc
     2221 gaggccatcg tagccacgat ctcgaagccc tggcgcttgt gaaagttctc ctccttgcag
     2281 atccgaacca ttgcgcgggc gtacggtccg tacgatgcct tagcgagcac agtctgattc
     2341 acgatagcgg ctccgtcgac gaaccagccg atcacgccaa tgtccgccca cgtcaaggtc
     2401 ggataattga agatgctgga gtacttcgcc ttgccggtga ggagctggtc gatcaactga
     2461 gcccgatcga cgcccagggt ttcggtggcg caatagatgt aaagcccgtg tccggcttca
     2521 tcctggacct tggccagcag acacatcttc cggcgcagcg acggagcgcg ggtgatccag
     2581 ttcccttcgg gcagcatacc cacgatctcg gagtgagcgt gctgggacat catccgcatg
     2641 agttgctgcc gatatcgctc cggcatccag tccttgggct cgatcgtctc accggcggcg
     2701 atacgagcct cgaaggcggc gttggaagcg ttgtcttccc ttgtgggcgt ggctgccatc
     2761 tcgttgtgcc tcatcagctt aaccggactc cgccgagtgc ctggaaagtc cctcggcagc
     2821 tgctaccttg catttgcaat tgtaccggtc cccgtgagcg aagggaagcc gtgactcgcc
     2881 aatccgcgca ggaccagggc agcgtcgcaa gtcacgtagt tgatggcgtt gctaccgtca
     2941 tcttcggtca tcccaaggga aattcactcc ccggcaagtt gctggccgat ctggctgcca
     3001 gcatcggcga cctcggagcc gatgataatg tcaacgtcat cgcgctcagg agcgacggca
     3061 ctggggcctt ctgtgccggc gcgtccttcg ccgagctggc cgcaatctcg gatgtcgagg
     3121 gtggcacccg tttcttcatg gggttcgcca atctgatcaa cgccatgagg gagtgcccca
     3181 agctcatcgt cacgcgcgta catggcagag ccgtgggtgg cggagttggt atcgtagctg
     3241 cttccgacta cgcacttgcc accactggag cacaagtaaa gctgagcgag cttatggtag
     3301 ggatcggccc cttcgtcgtc gggcctgtca tcgagcgaaa gatcggcgct gcgaactacg
     3361 ctgcgcttgg catcgacgcg gcgagttggc ggagcgcgga gtgggcgtat gagcgtggac
     3421 tctttgccga gctccatgcc aacgtcgccg acctcgacac cagaattgct gccctgacga
     3481 gtcaactcgc ggcaagcaat cccgctgcaa tggcggagct aaagaaggtg ctgtggcagg
     3541 gatgtgatca ctggcgtgag ctcttgcccg cacgagctcg gctgagcggc acgctggtag
     3601 tttcggcccc ggcccgccag gccatccagc gtttttccag ttagcaatgg accggtgcac
     3661 gcaggccggc ctgcgccgac agcacccaag aaaaaaagcc cgccgagtca ttgccgaccc
     3721 ggcgggcttt ggttacggac ttactgcaca gccctgagcg cgtccacgaa cccgtgcccg
     3781 taaaccgcat cgaagccagg cttgccatcg tcgtcagagg actggagcag cgctgccttc
     3841 agctgatcag gcgtcatgga tcctgaccca ccgtgcctgg cgaggatcag cgccgcgacg
     3901 cccgcaacat ga
//
LOCUS       DASRJQ010000595         6158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_4129154, whole genome shotgun sequence.
ACCESSION   DASRJQ010000595 DASRJQ010000000
VERSION     DASRJQ010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6158
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4129154"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            504..716
                     /locus_tag="VFB89_13485"
     CDS             504..716
                     /locus_tag="VFB89_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HZM28354.1"
                     /translation="MRTTGTVKWFNDAKGFGFITPEGGQKDCFVHHSAIQAQGFKTLA
                     EGERVEFDIVQGQKGPAAENVVKLGK"
     gene            complement(790..1176)
                     /locus_tag="VFB89_13490"
     CDS             complement(790..1176)
                     /locus_tag="VFB89_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28355.1"
                     /translation="MSDSIAGIVQVVGVAALPRIVLVREGAPTLTLSGPASLANLKGL
                     KIGVVGQVDGSTLAVSRFTVLEANGLSATDGRLIAEGDDLYLETADHVRHRLVNPSPR
                     LRERVGHRVWVSGPLDKEPVAYGFID"
     gene            complement(1287..3455)
                     /locus_tag="VFB89_13495"
     CDS             complement(1287..3455)
                     /locus_tag="VFB89_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28356.1"
                     /translation="MMGRVRWFTIVGPLGLALAACGGDTTAPPPNPPTITLSVNQVAF
                     AAPQGGAASPPQTVAVGNGGGGLLSDLTVGPITYGSASGWLTANLDKTIAPTTLTLTA
                     NPGGLASGSYTATVPVASASAGNTPQTITVALLVLSGNETTTISAAGGSTVFLDSPNL
                     GTQLTVQGGNQFLIAVVNTAATASGTEDFRLAGALIVEGAAATQPAAARPATRYVPPE
                     SPRIPTFAASQSVVVQRPLLERLAQNHLTALERNRELYARRGNPRDARARISALSGRR
                     APVAAAMSTTIGVVNKVYVSKGLGGDCTDVDSIGARTVAVGQHIIVLADTNRTTWPQA
                     DRPDSSFYQTFADEYDQITWPHLMANIGDPLAFDPNLSGVGKVTLTITPLLNSIGGGL
                     LAFVNGCDFFPFASTGPDADFSNFTEMFYSMVPSSSTFSVVNWEKQLRATAAHETKHI
                     VSFADRILNNSPAFEEIWLEEGLAQVSSEIWMRHFNQATWKGNADFRQTVGCELHLGP
                     SAPCNSQNDKPLDLVISHLPFLFEYLQKESDTHNEGLGQDLASDYGAGWSFARWTIDQ
                     YATDEGTFIKGLINEPSLIGVANLSSHSGQPGSLLLTFWNMATGIYNIPSYTPADPRI
                     TTPSFNFPEIFNVGQTQLTCGGTPCGLFTQSGEPVFPVQPIAISGAFNQSVQGLRGTS
                     AAFFLLSAASSGKQALQLQSGGGGTLSPSSSLRVGIIRVH"
     gene            complement(3487..4341)
                     /locus_tag="VFB89_13500"
     CDS             complement(3487..4341)
                     /locus_tag="VFB89_13500"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HZM28357.1"
                     /translation="MDLSLFLPPLVACLVIVAIHSYLGLHVIAREVIFVDLSLAQMAA
                     LGGAVAVLAGSQPDSQSAFLYSLGFTTIGAMLFALSRSDEKGRVPQEAIIGIVYVVAS
                     AAAILVADRTPRGGEAIKDILVGSLLWVTWPTIGRLAAIYAAVGLFHWLLRNRFLTIS
                     FHPEQALAQGWRIRWWDFLFYFSFGLVITFSVPVAGVLLVFSFLVIPAAIAFQFTRHK
                     RMLAAVSWLAGVLASAGGLWISFRYDLPTGPLVVCAFGVLLLAAYLVRRMLGIRIEEP
                     LTPLPERG"
     gene            complement(4341..5363)
                     /locus_tag="VFB89_13505"
     CDS             complement(4341..5363)
                     /locus_tag="VFB89_13505"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HZM28358.1"
                     /translation="MLIASLFIAHLLGGPSAAPVKIVTSLTTYGAIAKEIVGDRGTVT
                     SIAQGDEDPHFVQPKPSFVAVLRDADLFVTTGMDLELWVPALLDRAGNHKVSEGGPGF
                     VAAYQGIALLEVPTNLSRAAGDIHVDGNPHIHTDPLNGMIIARNILQGLKRVAPENAA
                     FFAQREQEFEKKVLEATMGPELVGILTPATAFDLLKNDKLSDFLAQQKYQGKPLSDRL
                     GGWLKEGQVLQGKEVACYHKEWAYFSHRFGVSCAQYIEAKPGIPPTPGHVQEVIAYMK
                     QHKLQVLFASNYFDRKQIETVATRTGAKAVIVPENTEGAPGVHTYVDLITTWIRGLTA
                     AFNGAS"
     gene            complement(5374..>6158)
                     /locus_tag="VFB89_13510"
     CDS             complement(5374..>6158)
                     /locus_tag="VFB89_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28359.1"
                     /translation="GVEEGYIYWTGLPGHIRLDVGKFRQQLGDLNRWHLHALPETEYP
                     LVYQRFLGQEGLAAVGVSLYSALPFSIAGGTHELFIQATTAESQPLYENSHQPTLLGR
                     FQNFWQLSRSTYAQLGFTATGGNNSDLDLQSRLLGLDFRLTYRPPNAGTRRDVTFRAE
                     GYRLHASEAGVTTDRYGAFLDLVARLSQRWILSGRYDYSQSPRGAQDTQWALIPAITW
                     WQSEFVFLRLEGQHLNDELTGNQNLLSFQVVFAMGPHKHETY"
BASE COUNT         1183 a         1848 c         2039 g         1088 t
ORIGIN      
        1 gaaccgacga acggcggggg tgtgagcggg ggcggctggt gcaggggact ggctggcgaa
       61 agcgttttgc gagagcgcca gccatcccgc gaagagcaac gcccgccggc aggatagaac
      121 gcaacgacga agtgaggtct gcattacgct tccagcttct tgcggaggtc tgtcacgatg
      181 gacgcttggt aaaggggggt cgggtgctcg ggctccctag gatagcgtcc tcgcctgtgt
      241 gcaggtaggc cagcagcccc tccggttcgc ggtcccccgc ttgcgaaccg gcacgtcggc
      301 cgatatcttg tgccgcgatc cgacgtgagt atggaggggc tggcgttggt gccggccctt
      361 tttgacttcc tgccgtttca cgtgcgcggg ttcgcgccgc ggcaggcagc ccagcgtgag
      421 ggttcagttc acgcacagag cgtagtcggt cggtacggcg catgtccgca ctccgattac
      481 cgcatccaca aggagtagga acaatgcgta cgaccggcac ggtcaagtgg ttcaatgacg
      541 ccaagggctt tggcttcatt acccccgaag gcggacagaa ggactgcttc gtgcaccatt
      601 ccgcgatcca ggctcagggc ttcaagaccc tcgccgaggg cgagcgggtc gagttcgaca
      661 tcgtgcaggg ccagaagggc ccggcggcgg agaacgtcgt caagctcggc aagtaatccg
      721 gaatccgggt actgaccgcg caatgcggcg ccggccggct caatagagtc ggccggcgtt
      781 gttttgccgt cagtcgatga acccataagc aaccggttcc ttgtcgagcg gaccggacac
      841 ccagacacgg tgaccgaccc gctcgcgcaa tctgggtgag ggattgacca gccgatgccg
      901 gacgtggtcg gcggtctcga gatacagatc gtcgccttcc gcgatgaggc ggccgtccgt
      961 cgcgctcagc ccgtttgcct cgaggaccgt gaatcggctg actgcgagtg tcgacccatc
     1021 gacctgtccc acaaccccga tcttgagccc cttcaggttt gccagcgacg caggtccgct
     1081 caaggtaagc gtcggcgctc cttcgcggac cagcacgata cgaggcaagg ccgcgacgcc
     1141 gacgacctga actatgcctg cgatgctgtc gctcaccgct tcgctccgcg gcggttccgg
     1201 cacatcggat cgatgccggc acgcggcggc catgaccacg acggccgcgc atccggcagc
     1261 aagacgccgc cccactccgt gcgacatcag tgaacccgaa taatgccgac cctgaggcta
     1321 ctggagggtg acagggttcc ccctccacca ctctggagct ggagggcctg ctttccactg
     1381 ctcgcggccg agagcaggaa aaatgcagcg gaagttcctc ggagaccctg cacgctttgg
     1441 ttgaaagcgc cggaaatcgc gatcggctgg actggaaaaa ccggctcacc cgactgcgtg
     1501 aacagcccgc agggcgtacc accgcaagtc aactgcgtct gacccacgtt gaaaatctct
     1561 ggaaagttga agcttggggt ggtgatccgc gggtcggcgg gggtgtacga cgggatgttg
     1621 tagatgccgg tagccatgtt ccagaaagtg agcagcagcg aaccgggctg cccgctatgg
     1681 ctgcttaggt tggctacgcc aatgaggctg ggctcgttga tcaacccttt gataaaggtg
     1741 ccttcgtctg tggcgtattg atcgatagtc caccgggcga acgaccatcc cgctccataa
     1801 tccgacgcca ggtcctggcc caggccttcg ttgtgggtgt cactctcctt ttggaggtat
     1861 tcgaacagga atggcaagtg actgatgacc agatcgagcg gcttgtcgtt ctgcgaattg
     1921 cacggggcgc tcggacccag gtgaagctcg cagcccacgg tctgccggaa atcggcgttg
     1981 cccttccagg tggcctgatt gaaatggcgc atccagatct cggacgacac ctgcgccaga
     2041 ccttcctcca gccagatctc ttcgaatgca ggactgttgt tgaggatacg atcggcaaac
     2101 gaaacgatgt gcttggtctc gtgggcggca gtggcgcgca gctgtttttc ccagttgacg
     2161 accgaaaacg tactcgacga gggaaccatc gagtagaaca tctcggtgaa gttgctgaaa
     2221 tcggcgtccg gtccggtcga cgcgaaggga aagaagtcgc acccgttgac gaaggccagg
     2281 agcccgccac cgatcgaatt caggagcgga gtgattgtga gcgtaacctt accgaccccg
     2341 gagagattag ggtcgaacgc gagcggatcg ccgatgtttg ccatgagatg gggccaggtg
     2401 atctggtcgt actcgtcggc gaaagtctgg tagaacgacg aatcggggcg atcggcttgc
     2461 ggccaggtgg tccggttggt gtctgccagc acgatgatat gctggcctac ggcgacggtc
     2521 cgtgccccga tcgagtccac gtcggtgcag tcgccgccca gccccttgct cacgtaaacc
     2581 ttgttcacaa cgccaatcgt cgtcgacatg gcggccgcta ccggtgcgcg ccggccgctc
     2641 aacgcgctga tgcgcgcgcg ggcgtcgcgt ggattgccgc gccgcgcgta caactcgcgg
     2701 ttgcgttcga gcgcagtcag atgattctgc gccaggcgct cgaggagcgg ccgctgcacg
     2761 acgacggatt gggaggcggc aaaggtcgga attctgggcg attccggcgg cacataccga
     2821 gttgcgggcc gcgccgcggc cggctgggtg gcggcggctc cctcgacaat cagcgccccg
     2881 gcgagtcgga aatcctccgt ccccgaggcc gtcgcagcag tgttgaccac ggctatcagg
     2941 aattggttgc caccctggac ggtgagctgg gtacccaggt tcggcgagtc gaggaatacc
     3001 gtcgatcccc cggccgcgct gatcgtggtg gtctcgttgc cgctgagcac gagcagcgcg
     3061 acggtaatcg tctgtggggt gttgcctgcc gaggccgagg cgacgggcac tgtagcggta
     3121 tagctgccgc tggccaggcc gccggggttg gccgtgaggg tcagggtcgt cggtgcgata
     3181 gtcttgtcga ggttggccgt gagccagccg gaggcacttc cgtacgtgat ggggccgacg
     3241 gtcaggtcag acaacaggcc accacccccg ttgccgacgg cgacggtctg cgggggagag
     3301 gccgcgccac cttgaggcgc ggcaaaggcc acctgattga ctgagagcgt tatcgtcgga
     3361 gggtttggcg gaggagccgt ggtatcgcca ccgcatgccg ccagggcgag cccgagagga
     3421 ccgacgatcg tgaaccaccg cacccgtccc atcatggcaa cctccgagag tcacgccgga
     3481 tatcggctac ccccgctcgg gtaggggagt gagtggctct tcgattcgaa tgccgagcat
     3541 tcgccgaacc agataggccg cgagcaacag cacgccgaac gcacagacca cgagcggccc
     3601 ggtcggcaga tcgtagcgga acgagatcca caatccaccc gcggacgcca gcacgccggc
     3661 cagccaggaa acggcggcga gcatgcgctt atgccgggta aactggaatg cgatggctgc
     3721 cgggatcacc agaaacgaaa ataccagcag cacaccagcc accggcaccg agaacgtgat
     3781 caccagcccg aaggagaagt aaaaaagaaa atcccaccag cgaattcgcc agccctgggc
     3841 cagggcctgc tcgggatgaa aggaaatcgt cagaaagcgg ttgcgtaaga gccagtggaa
     3901 cagccccacc gcggcataga tcgccgccag ccgcccgata gtcggccagg taacccacaa
     3961 caggctgcct accaggatgt ccttgatcgc ctcgccgcct ctgggggttc ggtcggccac
     4021 caatatggca gcggcggagg caacgacgta aacgatgccg atgatcgcct cctgcggaac
     4081 gcgccccttc tcatcgctgc gcgagagcgc aaacagcatt gcgccgatgg tggtaaagcc
     4141 gagcgaataa agaaaggcgc tctgggaatc aggttgacta ccggcgagca cggctacggc
     4201 tccgcccaat gcggccatct gagccagcga cagatcgacg aagatcacct cccgcgcgat
     4261 gacgtggagg ccgagatacg aatgaatcgc cacgattacc aggcaggcga ccagcggcgg
     4321 cagaaaaagg ctcagatcca tcaggacgct ccgttgaacg cggcggtcag gccacggatc
     4381 caggtggtga tgaggtcgac ataggtgtgc acgccgggag cgccctcggt gttttcgggc
     4441 acgatgaccg ccttcgcgcc ggtccgggtg gcgacggtct cgatctgctt ccggtcgaaa
     4501 tagttggagg cgaacagcac ctggagcttg tgctgcttca tgtacgcgat cacctcctgc
     4561 acgtgcccgg gcgtcggagg aatgcccggc ttggcttcga tgtactgggc gcagctgact
     4621 ccgaagcgat gactgaagta ggcccattcc ttgtgatagc aggccacctc cttgccctgg
     4681 agcacctgtc cctccttgag ccacccgccc agtcggtcgg agagcggctt gccctggtac
     4741 ttctgctgag ccaggaaatc ggaaagcttg tcgttcttga gcaggtcgaa tgcggtggcc
     4801 ggcgtcagga tcccgaccag ctccgggccc atcgtggcct cgagcacctt cttctcgaac
     4861 tcctgctccc gctgcgcaaa aaacgcggcg ttctcgggtg caacccgttt gaggccctgc
     4921 aggatattgc gggcgatgat catgccgttg agcggatcgg tgtggatgtg cgggtttccg
     4981 tccacgtgaa tgtcgccggc ggctcggctc agattcgtgg gcacttcgag cagggcgatt
     5041 ccttgatacg cggcgacgaa gccgggtccg ccttcgctca ccttgtggtt tcccgcccga
     5101 tccagcagag ccggcaccca gagctcgagg tccatgccgg tcgtgacgaa gagatcggcg
     5161 tccctgagga cggcgacaaa gctgggcttg ggctgcacga agtggggatc ctcgtccccc
     5221 tgcgcgatcg atgtgaccgt tccccgatcg cccacgattt ccttggcgat ggcgccatat
     5281 gtcgtcaggc tggtcacgat cttgaccggc gccgcggatg ggccgccgag caggtgtgca
     5341 atgaaaagcg acgcgatcag catcggatgt cctttagtag gtctcgtgtt tgtgggggcc
     5401 catcgcaaag accacctgga acgaaagcag gttctgattg cccgtgagct cgtcgttgag
     5461 atgctggccc tcgagtcgca ggaaaacgaa ttcactttgc caccaggtaa tggccgggat
     5521 cagggcccat tgcgtgtcct gtgcaccgcg gggcgattgg gaatagtcat aacggccgct
     5581 caggatccag cgttgcgaaa gtcgcgcaac gaggtccagg aacgcgccgt atcggtcggt
     5641 ggtcacaccc gcctcgctcg catgcagccg atagccttcg gcgcgaaagg tcacatcgcg
     5701 gcgagtgcct gcattggggg gccgataagt cagacgaaag tcgaggccca agaggcggct
     5761 ctgcagatcg agatcgctgt tgttaccgcc cgttgcggta aagccgagtt gggcgtaagt
     5821 ggagcgggag agctgccaga aattctgaaa gcgtcccagc agagttggct gatgactgtt
     5881 ctcgtagagc ggttggctct cagcggtggt tgcctgaatg aacagctcgt gagtgccgcc
     5941 ggcgatcgag aagggaaggg cgctgtagag cgagacgccg acagcggcga ggccttcctg
     6001 gccgagaaac cgctgataca cgagcgggta ttcggtttcg ggcagcgcgt gcaggtgcca
     6061 acggttcagg tcgccgagct gctgacggaa ttttcctacg tcgagccgga tgtgcccggg
     6121 aaggccggtc caatagatgt atccctcctc gactccca
//
LOCUS       DASRJQ010000596         9038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2062264, whole genome shotgun sequence.
ACCESSION   DASRJQ010000596 DASRJQ010000000
VERSION     DASRJQ010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 9038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9038
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2062264"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2720
                     /locus_tag="VFB89_13515"
     CDS             <1..2720
                     /locus_tag="VFB89_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZM28360.1"
                     /translation="LSSDDPRYDATYLSNYAKLYLEDEIKRLPGVGDGSVYGNLEFSM
                     LVSLDPQRMAQLGLTVTDVQNVVLQQNATKPAGRLGREPSPQSTQLTLPVTTTGRLTD
                     PGEFANLIVRARPDGSVVRVGDIGTVRLGSRNYDLVARVDGKPTAIIVVYARPRANAL
                     DVKNEVVGRMTELAKAFPPGVRWSLSFDTTPFITASIEEVVQTLLEAMVLVTLVVFVF
                     LQNWRATLIPILAVPVSIVGTFLGLAALGFSVNTLTLFGLVLAIGIVVDDAIVVIENV
                     ERIMEQEHVSPRVAADRAMRQVGGALVAIVLVLCAVFVPLAFLGGITGKMYQQFSLTI
                     VIAVVLSGIVALTLTPALCSVLLRDSHAKQENRFFRWFNQGFDWATRRYTGMAGRVVA
                     RPRTWVAVFAVMLALTVVLYRHVPGGFLPGEDKGYFAMAVQLPDAASTHRTEAVVRKI
                     EEMLKQEPSVDHVTAPVGLDVLTRANQTNYAVVFVTLKPWDERTGKDQSLEAILARMN
                     GRLATIKEALAFGFNFPEIPGLGATSGLELNLQQRTGGDIGAFAQQVQSFIQDASQLP
                     EVGGPRSLIRTDVPQVFVKVDDDAARARGVDQGQLFGTLQAMLSTLYVNDFNLYGRTY
                     RVQLEAQSRFRQRPEDIGGFYVRSDRGEMVPLSALVQAEMRGGPTLVSRFNGFPAALV
                     TATTTPGHSSGQMLDAVERLINQKYAPQGIGFAYSGQSYQERASAGQGGLIMGLGLML
                     VFLVLAAQYESWSIPIAVLFGLPFGAMGALVGIWLRGMANDVYFQAGLVAVIGLAAKN
                     AILIVEFASELHRQGRSITDAAIEAARERFRPILMTSFAFIVGVAPLAIAGGAGAGAR
                     RSLGTGVFAGMLTATLVGIFFIPLFFTVVSRLTERRSRATAALPVPEEA"
     gene            2720..4195
                     /locus_tag="VFB89_13520"
     CDS             2720..4195
                     /locus_tag="VFB89_13520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZM28361.1"
                     /translation="MRLAPVVLLLLPAMVGCAVGPSTRAAQPPAPALSARDAGAASSP
                     SRAFLDSLTRAREAEQPDTAASLLRPAPVALDSAGDLAWLGILKDSQLVTLVRTAVAN
                     NRDVRTATARVREYRAQLGVARADLFPQVDLNFSASTNKVAIGGFAPMQYDAVRITSD
                     LQWELDFWGRIRRQTEAAAFDWRAQEQDRRATVLSLVSDVATAYLELLEIDQNLTTSQ
                     QTLESRQATLQLARKRFEQGVISDLDVSQFESAVAAPAAQVAEFTRQRIEKENLLSVL
                     IGSPPRSIARGGRLEAAVSAVAVPDSLPAALIVRRPDVLRAQHDWQAATARVGAAVAD
                     RLPTFFVTAEYGTDRPDFHQLFGSSAEIYSVEGGVSVPLFHGGKLLNRERAARARADQ
                     AKSQYEQTVLTALREASDALAGVRLNRDQIAAQQTEVRALRRAYDVAQRRYQRGISSY
                     LDVLDVQRSLFDAELSLTQRERQYLAATVQLYKALGGGWTG"
     gene            4262..4708
                     /locus_tag="VFB89_13525"
     CDS             4262..4708
                     /locus_tag="VFB89_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HZM28362.1"
                     /translation="MTLALRKALYTAEATVRGGRDHGHGRSADGMIDVDLRIPVEMGG
                     PGGGTNPEELFAVGYAACFQSAMGVVGRRLGVDTGDSTITSRVTVGPIEGGAFGMAVE
                     LQVELPGVGRAVAQRVVHDAHQLCPYSNATRGNIDVSLVVLDRSPS"
     gene            4705..5595
                     /locus_tag="VFB89_13530"
     CDS             4705..5595
                     /locus_tag="VFB89_13530"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZM28363.1"
                     /translation="MIGSKKSGARFWPPAPQAARDVACAHLEAGQAQPHVHEDWQFAV
                     ADIPAEVCVAGRWRYTVGPSDITVVAPYEVHSECGAGRHAPHWRMLLVPPSILSIACQ
                     GLPANQKCEHRRFTSEVLPDPAGALALRRLLLDSEDGLLDGVEFVTRALPWLDDLLDR
                     HRGDGGRPPRSRAVERAHAYIRRRATRSVTLAEVAIAAGVAVGHLVRSFSAAVGLPPM
                     SYQTQVRVARSKRLLAQGVSASSVAYECGFADQSHLIRHFKKCYGLTPGAFQKQYCDQ
                     QPVARGTTDSGVPEVESNAA"
     gene            5585..5995
                     /locus_tag="VFB89_13535"
     CDS             5585..5995
                     /locus_tag="VFB89_13535"
                     /inference="COORDINATES: protein motif:HMM:NF027640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HZM28364.1"
                     /translation="MPREQALPAPKPEMRLGHAHGNGYYERLPAALAVLSIVRNSLAH
                     AFSLAGVVAAIRFRNKLKESRDAVYIFTAIAIGFAAGVRELGVALILSVLFSATELAL
                     WRLDLIEEHTASFRRLLTEEPTVANSQRAGERLA"
     gene            6021..6299
                     /locus_tag="VFB89_13540"
     CDS             6021..6299
                     /locus_tag="VFB89_13540"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HZM28365.1"
                     /translation="MTLVRATQILLWASWYHWLCFQSWESALYFSATTYATVGYGDVI
                     LPSEWRLLGPVESIIGVLMCGVSVSLLFAIATELVGQGKILAQPNAVD"
     gene            6369..7547
                     /locus_tag="VFB89_13545"
     CDS             6369..7547
                     /locus_tag="VFB89_13545"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HZM28366.1"
                     /translation="MSVQCAQFIQRSAFEMVLGVVVVLLGGITLAHPLWAQADTAGDS
                     GHATASGDSLPARRAPTPQRKAIYFYPVPTGEQLTGLKLTLVGAYLWRTAMDGTTRPS
                     AIFHHSAFTTKHQVLIGFGGDLWAPQNTKHLNFGIEYSRFPAPFYGIGPHTPQSAEEL
                     FTPRTFTVGFTAQREFRPHTYVQLGWHVLHASVVDRAPGGLLIGDTIPGSSGYTEVAP
                     EIGLVFDNRDRLFDPRSGAFIEAHARVNLPPLGGSSGYQLYLLDSRVYRTVRPGSVLA
                     MQLAFAGASGTVPFEELPQLGGSELRAYVYGRWRDRVALSGQAEWRQHVVWRCGVVAF
                     VGAGVIGPDVASFGPVRPTYGAGFRFNLLTKEDVNFSMDYARGQEGASALSFGFAEAF
                     "
     gene            complement(7566..7639)
                     /locus_tag="VFB89_13550"
     tRNA            complement(7566..7639)
                     /locus_tag="VFB89_13550"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7603..7605),aa:Arg,seq:cct)
     gene            complement(7751..8122)
                     /locus_tag="VFB89_13555"
     CDS             complement(7751..8122)
                     /locus_tag="VFB89_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28367.1"
                     /translation="MAIHSQQGPKPTPGVAITIPESSKVSPDSPTSADPPELSNQRPA
                     AAEPVVPPDASESKPPRSSRAKAIWGLAVTSLSPGIVVGVAPAFWHGLPTWAQWSAYA
                     VSGVLMLLVVGLIVTPRRATE"
     gene            complement(8248..8601)
                     /locus_tag="VFB89_13560"
     CDS             complement(8248..8601)
                     /locus_tag="VFB89_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28368.1"
                     /translation="MQRARGSVWFAVIGGMLAWSVRTIPPSPPVSGAAIAAVAEAPLP
                     LANRLRPQLVDFSPDLRGRATERGPTLDLLASSQITPEASNKRLLIEPARLVTHVSSA
                     AGALPYFPTGPPPIA"
     gene            complement(8626..>9038)
                     /locus_tag="VFB89_13565"
     CDS             complement(8626..>9038)
                     /locus_tag="VFB89_13565"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HZM28369.1"
                     /translation="TGPMSSGNGSAGNPGASGGSGGGGSGGDTGGNTSGGTPTNGDVA
                     VLVGNNFMKSGHNGSVNPAVDTVSAGGSVTWTWTRTGDVPHSIHSLGNPAFPSSAILT
                     GDGSVYRVTFKAAGTYRYDCAVHGSMMSGTIVVQ"
BASE COUNT         1500 a         2935 c         2969 g         1634 t
ORIGIN      
        1 ccctctcctc ggatgatccg cgatacgacg cgacctacct cagcaactac gcaaagctct
       61 acctcgagga cgagatcaag cgactccccg gcgtgggcga cgggtccgtg tatgggaacc
      121 tcgagttctc gatgctggtg agcctcgatc cgcagcggat ggcgcagctc ggcctcaccg
      181 tgacggacgt gcagaatgtg gtgctccagc agaacgccac caagcctgcc ggacggctgg
      241 gccgcgagcc atcgccgcag tccacccagc tgacgctgcc ggttaccacg accggacggc
      301 tgaccgatcc cggcgagttt gccaacctca tcgtccgagc tcgacctgac ggctcagtgg
      361 ttcgggtcgg ggacatcggc acggtcaggc tcgggtcgcg gaattacgac ctggtcgccc
      421 gagtggatgg caagcccacg gcgatcatcg tggtgtacgc ccgcccccgt gccaacgcac
      481 tggatgtgaa gaacgaggtg gtgggacgca tgaccgagct tgccaaggcg ttcccgcccg
      541 gggtgcgctg gagtctgtca ttcgacacca ccccgttcat cacggcctcg atcgaagaag
      601 tggtccagac cctgttggaa gccatggtcc tggtgacgct ggtcgtcttc gtgttccttc
      661 agaactggcg cgccacactc attcccattc tggccgtgcc ggtgagcatt gtcggcacct
      721 tcctcgggct cgccgcactc ggcttcagtg tgaatacgct cacgctgttc ggcctcgtcc
      781 tcgccatcgg gattgtggtg gacgacgcca tcgtcgtgat cgaaaacgtc gagcgcatta
      841 tggaacagga gcacgtctcc ccacgagtgg cggcggaccg cgctatgcgc caggtgggag
      901 gcgccctagt cgccatcgtg ctggtgctct gcgccgtgtt cgtgccgctc gcattccttg
      961 gcgggattac cggaaagatg taccagcagt tctcgctcac gatcgtgatc gcggtggtgt
     1021 tgtccgggat cgtggccctg acgctcaccc ccgccctctg ttcggtcctg ctccgggact
     1081 cgcacgccaa gcaggagaac cgattctttc gctggttcaa ccaggggttt gactgggcca
     1141 ctcggcgcta caccgggatg gccggccggg tggtcgcgcg gccgcggacc tgggttgccg
     1201 tcttcgccgt catgcttgca ctcaccgtgg tgctctaccg gcacgtccca ggcgggttcc
     1261 tgcccggcga ggacaagggg tatttcgcca tggcggtcca attgcccgac gcagcatcga
     1321 cccaccggac cgaggcggtg gttcggaaaa tcgaggagat gctcaagcag gagccctcag
     1381 tggaccacgt gacggcgcca gtgggcctcg atgtcctgac ccgggccaac cagaccaact
     1441 acgccgtcgt tttcgtcaca ctcaaaccgt gggatgaaag gaccggtaag gatcagagcc
     1501 tggaggcaat ccttgctcgc atgaacgggc ggctcgccac catcaaggag gcgctcgcct
     1561 tcggcttcaa tttccccgag atcccgggac tgggagcgac cagcggcctc gagctcaacc
     1621 tccagcagcg cacgggtggt gacatcggtg cctttgccca gcaggtccag tccttcatcc
     1681 aggatgcaag ccaattgccg gaggtcggag gtccacgatc cctcattcgt accgacgtgc
     1741 cccaggtgtt cgtcaaagtc gacgacgacg ccgcccgggc ccgcggcgtc gaccagggac
     1801 agctcttcgg cacactccag gcaatgctct ccacgctcta tgtgaacgac ttcaacctct
     1861 atggccgcac gtatcgagtg cagctcgagg cgcagtcgcg gttccgccag cgccccgagg
     1921 acataggagg cttctacgtg cgaagcgatc ggggggaaat ggtgccacta tccgcgctgg
     1981 tacaagccga gatgcgagga ggaccaacgt tggtgtcccg cttcaatggc ttcccggctg
     2041 ccctggttac ggccaccacg acgccgggcc acagctcagg ccagatgctc gacgcggttg
     2101 agcgcctcat caaccagaag tatgcgcccc agggcatcgg cttcgcctac agcggccagt
     2161 cctatcagga gcgcgcctcg gcggggcaag gcggtctcat catgggtctg ggtttgatgc
     2221 tcgtgttcct cgtgttggcc gcgcagtacg agagctggtc catccccatt gcggtcctgt
     2281 tcgggcttcc gttcggcgct atgggggccc tggtcggcat ctggctgcga gggatggcga
     2341 acgacgtcta tttccaagcc ggcctcgttg ccgtgattgg tctcgcagcc aagaacgcca
     2401 tcctgatcgt cgagttcgcg agcgagctcc atcgtcaggg gcgctcgatc acagacgccg
     2461 cgatcgaggc ggcacgcgaa cggttccgtc cgattctcat gacctccttc gcgttcattg
     2521 tgggcgttgc tccactcgcc atcgcgggcg gcgccggagc cggcgcccgg cgctcgctcg
     2581 gcaccggcgt gttcgccggg atgctgacgg ccaccctggt cgggatcttc ttcatcccgc
     2641 tcttcttcac ggtcgtcagc cggttgactg aacggcggag cagggcaacc gcggcgctgc
     2701 cggtgccgga ggaggcatga tgcggctcgc gcctgtcgtt cttctccttc tcccggcgat
     2761 ggtcgggtgc gcggttggcc cgtcgactcg cgcggcccag cctcctgcac ccgcgctgtc
     2821 ggctcgcgac gcgggagcgg cttcatcccc gtcgcgcgcc tttctggact ctctgacccg
     2881 cgcccgcgag gcggagcaac ccgacaccgc ggcgtcgctg ttgcgccccg cgccggtcgc
     2941 gctcgactct gccggcgacc tggcctggct cgggatactc aaggactcgc aattagtgac
     3001 gctggtccgg accgccgtcg ccaacaaccg cgatgtccgg acggcaaccg cacgggttcg
     3061 ggagtaccgc gcccagcttg gcgtcgcccg ggccgacctg ttcccgcagg tggatctcaa
     3121 cttctcggcc agcaccaaca aggttgccat cggtggtttc gctccgatgc agtacgacgc
     3181 cgtccgcatc accagcgatc tccaatggga gctggacttc tggggccgca tccgccgtca
     3241 gaccgaagcc gcggccttcg actggcgggc ccaagaacag gaccgccgag cgaccgtcct
     3301 ctcgctcgtg agtgacgtag cgactgccta tctcgagctg ctcgagatcg accagaacct
     3361 gacgacgtca cagcagacgc tcgaatcccg gcaggccact ctccaactcg cgcgcaagcg
     3421 gttcgagcag ggcgttatct ccgatctcga cgtcagccag ttcgagtcgg cggtcgcggc
     3481 gccggctgcg caagtggccg aattcacccg tcagcggatc gagaaggaga acctgctcag
     3541 cgtcctcatc ggcagcccgc ccagatcgat agcgcgcggt ggaaggttgg aggcggctgt
     3601 gagtgcggtg gccgtgccgg actcactgcc ggcagcgctc atcgtgcgcc ggccggatgt
     3661 gcttcgagcc cagcacgact ggcaggcagc aacggcacgg gtgggagcgg ccgtggccga
     3721 ccgcctgccg accttctttg tcactgccga gtacgggacc gaccggccgg acttccacca
     3781 gctgttcgga tcgagtgccg agatctacag cgtggagggc ggtgtttcag ttccgctgtt
     3841 ccacggcggc aagctgctca atcgggagcg agccgcgcgg gcccgagccg atcaggccaa
     3901 gtctcagtac gagcagacgg tgctcacggc actccgggaa gcgagcgatg cgctggccgg
     3961 ggtccggctt aatcgcgatc aaatcgccgc ccagcagacc gaagtgcggg cacttcgccg
     4021 agcatacgac gtcgcgcagc ggcgttacca gcgcggaatc tcgagctatc tcgacgtgct
     4081 ggacgtccag cggagcctgt tcgatgccga gttgtcgctg acgcaacgcg agcggcagta
     4141 cctcgccgcc accgtgcagc tctacaaggc gctcggtggt gggtggacgg ggtgaacgtc
     4201 agaccaatac cactaacgtc gcgcggctgc ggccgagcgg cgcgaaggag gaagttcgaa
     4261 aatgacactc gcgttgcgga aggcccttta cactgccgaa gcgacggtgc gcggcggacg
     4321 cgatcacggt cacggccgtt cagcggatgg gatgatcgat gtcgatctcc gcatcccagt
     4381 cgaaatggga ggtcccggtg gcggtaccaa tccggaggag ctgttcgcgg tcggatacgc
     4441 ggcctgcttc caaagcgcca tgggagttgt cgggaggaga cttggcgtgg acaccggcga
     4501 ctcgacgatc acctcgcgcg tgacggtcgg tccaatcgaa ggtggggcat tcgggatggc
     4561 ggtcgagctc caggtcgagc tgccgggtgt cggccgcgcg gtggcgcagc gcgtcgtcca
     4621 cgatgcgcat cagctctgtc cgtacagcaa cgcaacgcgc ggcaacatcg acgtttccct
     4681 ggtcgtcctg gaccgatccc cctcatgatc ggctcgaaaa agagcggcgc acgattctgg
     4741 ccgcccgcac cgcaagcagc gagggatgtc gcctgcgcgc acctcgaggc tggacaggcc
     4801 cagccgcatg tacacgagga ctggcagttc gcggtagcgg acattcccgc cgaagtgtgc
     4861 gtcgccgggc gctggcgata cacggtgggc ccctcggata tcaccgtagt ggcgccctat
     4921 gaggtgcaca gcgagtgcgg tgcgggcagg cacgcaccgc actggcgaat gttgctggtg
     4981 cctccctcga tcctctcgat cgcttgccag ggattgccgg cgaaccagaa gtgcgagcac
     5041 cgccgattca ccagcgaagt gctgcccgac cccgcgggcg cgctcgcgct ccgccgattg
     5101 ctgctggaca gcgaggacgg actgcttgat ggtgtcgagt tcgtcacgcg tgcgctgcct
     5161 tggctggacg acctgctgga ccgtcatcga ggggatggcg gaaggccgcc ccgaagtcgc
     5221 gcggtcgaac gggcacatgc ctatatccga cgtcgcgcaa ctcggtcggt gacactcgcg
     5281 gaggttgcga tcgctgccgg tgtcgccgtg gggcacctcg tgcgctcctt ctccgcggcc
     5341 gtcgggctgc ctcccatgag ctatcagacc caggtgcgtg tcgcgcgttc gaaacggctt
     5401 ctggcccaag gggtgtctgc gagctcggtc gcctacgagt gcgggtttgc cgatcagtca
     5461 catctgatcc gtcacttcaa gaaatgctac ggcctgacgc cgggcgcctt tcagaagcag
     5521 tactgcgatc aacagccggt cgcgaggggc acgaccgaca gcggggtccc ggaggtggaa
     5581 tcgaatgccg cgtgagcagg cgctacccgc gcccaagccg gaaatgagac tggggcacgc
     5641 acatggcaat ggctactacg agcgactacc tgccgccctg gcggttctca gcatcgttcg
     5701 caatagcctg gcccacgcat tcagcctcgc cggcgtcgtc gcggcgatcc gcttccgtaa
     5761 taagctcaag gaatcgcgcg atgcggtgta catcttcacg gcaatagcga tcggttttgc
     5821 ggcgggggtc cgagagctgg gcgtcgcatt gattctgtcc gtgctgttct ccgcgacaga
     5881 gctggccctg tggcgactcg atctcatcga ggagcataca gcgtccttcc gacgcctgtt
     5941 gaccgaggaa cccacggtgg cgaactcgca aagggccggc gaacggctgg cctaggggtg
     6001 attgtggcgt ggcccacgcg atgacgttgg ttcgtgcaac ccagattctg ctgtgggcga
     6061 gctggtatca ctggctttgt ttccagtcat gggagtcggc gctgtacttt tcggcgacca
     6121 cctatgcgac cgtcggctat ggcgacgtga tcctcccatc cgagtggcga ctgttggggc
     6181 cggtagagag catcatcggt gtgctgatgt gcggagtatc cgtaagcctt ttgtttgcaa
     6241 ttgccacaga gctcgtcggg caaggcaaga tcctcgcaca accgaacgca gtcgactgag
     6301 tctgagtcgt gtacaggcat cccgataccg tgacctgtgc gaacgcggat ctcacggaca
     6361 cggtcacaat gtctgtgcag tgtgctcaat tcatccagcg gtcggccttc gagatggtgc
     6421 ttggcgtcgt ggtggttctc ctcgggggca tcaccctggc tcaccctctg tgggcacagg
     6481 ccgacactgc tggcgattca gggcatgcca ctgcctcagg cgactcgctg ccagctcggc
     6541 gcgcgcccac gccgcaacgg aaagccatct atttctaccc ggtgcccacc ggcgaacagc
     6601 tgaccggcct caagctcacg ctcgtgggcg cctatctctg gcggaccgcc atggacggca
     6661 ccacgcgccc gagcgcgatt ttccatcatt cggccttcac cacgaaacat caggtgctga
     6721 tcggcttcgg gggcgacctg tgggcccccc agaacacgaa gcacctcaat ttcgggatcg
     6781 agtactcccg gtttcccgcc ccgttctacg gcatcgggcc acacacgccg caatcggcgg
     6841 aggagctgtt cacccctcgc accttcaccg tcggctttac ggcacagcgg gaatttcggc
     6901 cccacaccta cgtccagctg ggatggcacg tcctgcacgc gtccgtcgtg gaccgagccc
     6961 ccggtggact cctcatcggc gacaccatcc cgggaagtag cggctacacg gaagtcgcgc
     7021 cggaaatcgg cctggtattc gataaccggg atcgactctt cgatccacga agcggggcct
     7081 tcatcgaggc ccacgcgcgg gtcaatctgc cgccgctcgg gggctccagc ggctaccaac
     7141 tgtatctcct ggactctcgg gtctatcgca ccgtgcgacc cggctcggtt ttggccatgc
     7201 agctcgcatt cgcgggagcg tctggcaccg tgccgttcga ggagctaccc cagctcggtg
     7261 gcagcgagct ccgcgcctat gtgtacggtc gctggcgcga ccgcgtcgcg ctgagcggcc
     7321 aggccgaatg gcggcagcac gtcgtgtggc gctgtggcgt tgtggccttc gtgggtgcgg
     7381 gggtgattgg accggacgtc gcgtcgttcg gacctgtgcg cccgacctac ggcgccggtt
     7441 tccggttcaa cttgttgacc aaggaagacg tgaacttcag catggattac gcgcggggcc
     7501 aggaaggggc cagtgcgctc agttttgggt tcgccgaggc gttctaggag aatcagttac
     7561 ttcagtgccc ccggcgtgat tcgaacacgc ggccaacagt ttaggaaact gctgctctat
     7621 ccatctgagc tacgggggcg ggtaggtgat gcaatatctt ggcgttccca ccgagcgtaa
     7681 agtctcagcc gcgctcgcgg agtagcgccg ccggcgatgg cccgacggcg ctcatccgga
     7741 agaagtcacc tcactcggtt gcgcgacggg gtgtgacgat cagacccacg acgagcagca
     7801 tcaggacgcc ggagactgcg tacgcggacc attgcgccca ggtgggaaga ccgtgccaga
     7861 aggccggcgc gactccgaca acgataccag gtgacaagct ggtgacggcc agtccccaga
     7921 tcgccttggc gcgcgaggat cgcggcggtt tcgattccga ggcatccggc ggcactacgg
     7981 gttccgctgc agccggccgc tggttggaga gttctggtgg gtccgccgac gtcggactgt
     8041 ctggcgagac cttgctgctt tcgggaatag tgatcgctac cccgggggta ggcttcgggc
     8101 cttgctgtga atggatggcc acgacttgct cctgtttgac cggtacgaac gcaccgcgaa
     8161 cgcctccgga gaggcgcctc gcgccatgga tgagatcgga ggtgcggatc ggcccgcgcg
     8221 ccgtccggcg gggggcagcc gccctgtcta tgcgatcgga ggcggaccgg tcgggaagta
     8281 cgggagtgcg cctgcggcgg acgagacgtg cgtgacgagc cgggccggct cgatgagcaa
     8341 gcgcttgtta ctggcctcgg gagtgatctg gctcgacgcc agcagatcga gggtggggcc
     8401 gcgctcggtc gccctgcccc gcaggtcggg tgagaagtcg accagctgcg gccgaagccg
     8461 attggccagc ggaagcggcg cttctgcgac tgcagcgatc gcggcgccgg acaccggggg
     8521 tgagggcgga atggtccgaa cagaccatgc caacatgccc ccgatgacgg cgaaccagac
     8581 cgagccgcgt gcgcgttgca ttgcgggaat ctaagccagc ctggcctatt gcacgacaat
     8641 cgttccggac atcatgctgc cgtggactgc gcaatcgtag cgataggtgc ccgcggcctt
     8701 gaaggtcacc cgatagacgc tgccatctcc ggtaagaatc gccgagctcg ggaacgcggg
     8761 attgcccagt gagtggatgc tgtgagggac atctcccgtt ctggtccagg tccaggtcac
     8821 gcttcctcct gccgaaacgg tgtccaccgc aggattgacg gaaccgttgt gaccgctctt
     8881 catgaaattg ttgccgacca ggacggctac atccccattg gtcggggtgc cgccgctggt
     8941 attgccaccc gtgtcgccac cgcttccacc tcccccgctg cctccgctcg cacctggatt
     9001 cccggctgaa ccgttgccgc tgctcatcgg acccgtgg
//
LOCUS       DASRJQ010000597         5301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1124411, whole genome shotgun sequence.
ACCESSION   DASRJQ010000597 DASRJQ010000000
VERSION     DASRJQ010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124411"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..683
                     /locus_tag="VFB89_13570"
     CDS             87..683
                     /locus_tag="VFB89_13570"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HZM28370.1"
                     /translation="MSYDSRPAQLAVAFFVLAAFTPVSAAAQQRRYTPADVEFMEGMI
                     GHHAQAIAMAALVPGRTTNPTMQLLARRIDISQRDEIRLMQSWLRDRDQTVPDSTGQM
                     DHVNGHHEMLMPGMLTADQMAQLAAAKDTTFDRLFLQFMIQHHQGALTMVKTLFQSPG
                     AAQATDTFRYVSGVDSDQRAEIERMQNMLDAMPGSHQS"
     gene            748..2523
                     /locus_tag="VFB89_13575"
     CDS             748..2523
                     /locus_tag="VFB89_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28371.1"
                     /translation="MRRSWLLLLTAGMLVAPVGLTAQSDHYSPDAPRTNDPRVRLKPG
                     YDNAGTAIKNLKFVVHEPIMASFYDSATAGKNEFVNADMAFQGNYIFQGNYYGFQVWD
                     ISNPKKPVLRTSFVCPGGQGDPSVYHNLLFISVEETSGRLDCGRDGVTDTVSTERFRG
                     VRIFDISDLDHPKQVAAVQTCRGSHTHTLVTDPNDDQNVYIYVSGTSVVRSGNELAGC
                     SGKRPEEDPNTSLFRIEVIKVPVASPQDAKIVNQPRLFADSAGNIAGLWKGGTHGEGT
                     QTTAETNMCHDITTFQAAGIAAGACSGNGILLDIRDPANPKRITEVSDPNFAYWHSAT
                     FNNDATKVLYTDEWGGGSLPRCRASDPPKWGADAIYTLDDGKLTPASFYKLPAVQGDL
                     ENCVAHNGSLIPVPGRDIMVQAWYQGGVSVFDFTDAAHPVEIAYFDRGPQDPQKLGEG
                     GGYWSVYWYNGYIVGSEITRGLDLLELKPSDQLSKNEIEAAKLVKLEVFNPQAQPKIV
                     WPASFFVSRAYLDQLERNQGLNKDRLSQVKKELKNAEKKKGTEQVDALRKLAGELDQD
                     AQGAADPARVKLLASSVNDLAAATH"
     gene            complement(2574..3053)
                     /locus_tag="VFB89_13580"
     CDS             complement(2574..3053)
                     /locus_tag="VFB89_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28372.1"
                     /translation="MPRTDVGSRRWGFVLAGGLVAGTLDIVYACVFWALKADVPVQRI
                     LQSVAAGLLGQESFQGGASTAALGLGLHYFIATSMSVVYYLVARRWASSWQRPVLYGG
                     VYGLLLYVVMNYVVVPLSAAGPGSKNALWIGLSVLVHICLIGIPIALFARRALRDQT"
     gene            3201..4661
                     /locus_tag="VFB89_13585"
     CDS             3201..4661
                     /locus_tag="VFB89_13585"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HZM28373.1"
                     /translation="MEFHVSLIGRKDLSGEIYRQLRQAILDGRLRPGANLPPTRELAR
                     GLHVSRTTVTVAYDRLDAEGFVTSRVGAGTFVSHTAGRVMNQGKAHQVEGALRPKPVW
                     EAIPLTTPFARPARFDFRTGLPDASLFPHDRWRRLLARELRSEEVAAGVYGHPAGHRG
                     LREAMARHVGIARGVNAPADDIIITSGIQQAIDIVARVLLRPGDRVAIEDPCYPPPRR
                     LLESVGAKVVGVPVDDLGLVVDALPRHTRLVYVTPSHQYPLGVSMALPRRLALLAWAE
                     RHNAAIIEDDYDSEFRFSGRPIEPLQTLDTAGRVIYLGSFSKTMLPTLRLGFMVTPRS
                     LRTAVQKAKFVTDWHTALPLQAALARFIDDGEFARHVRKVGAVYRTRHTTLTAGLEKE
                     LGQHLQIVPCDAGLHVAAVARVASVREVAEAARRASEMGVEVQQLATFAVNPPGRAGL
                     VLGYGRIEAGRMEEGLRLIRQCFEQGGTLVIGSGVQ"
     gene            complement(4682..>5301)
                     /locus_tag="VFB89_13590"
     CDS             complement(4682..>5301)
                     /locus_tag="VFB89_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28374.1"
                     /translation="WDGDTLAAAESARRLEQTAGALPANHVGKPLAACVLEQWHLANR
                     QLATAPRTIAALRVFQPAQSDSQFVMIARGCAILLDAMLAGAERRPDAARKLGALDSL
                     MKTGPPYRSMNESWNLVTARLLEATGDRASALAATRRRLYDVAEPRFLSTYLREEGRL
                     AALTGDTAGAIRAYRHYLILQADPEPAARPRVQRVRAELATLTGG"
BASE COUNT         1049 a         1714 c         1647 g          891 t
ORIGIN      
        1 gctgcctaaa cgcattagat tgaaacacgg cctcgaaaca ccagccgccg atcgttcgta
       61 ggtcggctgg gccccataca aagcatatga gctacgactc tcgccctgca cagctggcgg
      121 tcgctttttt cgtgctggct gcgttcaccc cggtctctgc ggcggctcag cagcgccgct
      181 acacgcccgc cgacgtcgag ttcatggagg gcatgatcgg gcaccacgca caggcgatag
      241 caatggcagc gctggttcca ggccgcacga caaatccgac catgcaactg ctcgcgcgga
      301 gaatcgatat ctcacagaga gacgagatcc ggctgatgca aagctggctc cgggatcgag
      361 atcagacggt tccggattca accggccaaa tggatcacgt gaacggacat cacgagatgc
      421 tcatgccggg tatgctgaca gcggaccaga tggcgcaact ggccgcggcc aaggacacca
      481 ccttcgaccg tctctttctt caattcatga tccagcatca ccagggcgcg ctcaccatgg
      541 tgaaaacgct cttccagagc cccggcgccg cgcaggcaac cgatacgttt cgatatgtct
      601 cgggcgtaga cagcgatcag cgcgccgaaa tcgagcgcat gcagaacatg ctcgacgcga
      661 tgcctggatc gcaccagtct tagctgctca gtaccagacc atacaccccg atcgccaagc
      721 gagaacccga ccatgagatc agaggcaatg aggcgctcct ggctgctcct cctcaccgcc
      781 gggatgctgg ttgcgccggt gggactgacc gcacagagcg accactactc cccggatgcg
      841 cctcgcacca acgacccgcg agtacggctc aagcccggct acgacaacgc cggaacggcg
      901 atcaagaatc tgaagttcgt ggtccacgag cccatcatgg cgagctttta cgattcggcg
      961 acggcgggga aaaacgaatt tgtcaacgcg gacatggcct tccagggtaa ctacatcttc
     1021 cagggcaact actacggctt tcaggtgtgg gacatctcga accctaagaa gccggtcctg
     1081 cgcacgtcgt tcgtctgtcc cggcggccaa ggcgacccat cggtttatca caacctcctc
     1141 ttcatttcgg ttgaggaaac cagcgggcgg ttggactgcg ggcgggacgg cgtgaccgac
     1201 accgtcagca ccgagcggtt tcgcggtgta cgcattttcg atatcagcga tctcgaccat
     1261 cccaagcagg tcgccgcggt tcagacctgc cgtggctcac atacccacac actcgtcacc
     1321 gatcccaacg acgatcaaaa tgtctacatc tacgtgtcgg gaacgagcgt ggtgaggtca
     1381 ggcaacgaac tagccggctg ctccgggaag cgccccgagg aagatcccaa cacgtcgctc
     1441 ttccggatcg aagtcatcaa ggtcccggtc gcgtcacccc aggacgccaa gatcgtgaac
     1501 cagcctcgac tcttcgccga ctcggctggc aacatcgcgg gactttggaa aggcggcact
     1561 cacggcgagg gcacgcagac aaccgccgag acaaacatgt gccacgacat tacgacgttc
     1621 caggcagccg gaatcgcggc cggcgcatgc tcgggcaacg gcattctgct cgacatccgg
     1681 gatcccgcca atcccaagcg catcacagag gtgagcgatc ccaactttgc ctactggcac
     1741 tcggcgacgt tcaacaatga cgcgaccaag gtgctgtaca ccgatgaatg gggtggtggc
     1801 agcctgcccc gttgtcgcgc gagcgatccg ccgaagtggg gggcggatgc catttacaca
     1861 ctcgacgacg gcaagctcac tccggccagc ttctacaagt tgcctgccgt ccagggcgac
     1921 ctggagaatt gtgtcgccca caacggctca ctgatcccgg tgccgggccg cgacatcatg
     1981 gtgcaggcgt ggtatcaagg cggcgtctcg gtcttcgact ttaccgatgc cgcccatccc
     2041 gtcgagatcg catacttcga tcgagggcca caggacccgc agaagttggg agagggggga
     2101 ggctactggt ccgtctactg gtacaacggc tacatcgtag gctcagagat cactcgtggc
     2161 ctagacctgc tagagctcaa gccaagcgac cagctgtcca agaacgagat cgaggccgcc
     2221 aagctggtga agctcgaggt gttcaacccg caggcgcagc ccaagatcgt gtggccggcg
     2281 agcttcttcg tttcccgggc gtacctggat cagctcgagc ggaaccaggg gctcaacaaa
     2341 gaccggttgt cacaggtcaa gaaggagctc aagaacgccg agaagaaaaa gggaaccgag
     2401 caagtggacg cgttgcggaa gctggcgggc gagctggacc aggatgctca gggagccgcc
     2461 gacccggctc gcgtgaaact gctcgcaagc tcggtcaacg acctggctgc cgctactcac
     2521 tgatctggct ccacgggcag acgcggctcg cgttgagccg cgtctgtttt gtactaggtc
     2581 tgatcccgca gcgcccggcg ggcaaacaga gcgatgggaa ttccgatcaa gcagatgtga
     2641 accaggacgc tcaggccgat ccacagcgca ttcttggagc cgggccctgc ggctgagagc
     2701 ggcacgacga cgtagttcat gacgacgtac aacaacaacc cgtagacacc gccgtagagc
     2761 accggccgct gccacgacga agcccaccga cgcgccacca ggtagtacac cactgacatc
     2821 gacgtcgcga tgaagtaatg aaggcccagg ccgagcgccg ccgtagacgc gccgccctgg
     2881 aagctctcct ggccgagcaa accggccgcg accgattgca ggattcgttg gaccggtaca
     2941 tcggccttga gcgcccagaa cacgcaggcg tacacgatgt cgagcgtgcc ggcgaccagg
     3001 ccacccgcca agacgaatcc ccaccgccgc gatccgacgt cagttcgtgg catcgcaaag
     3061 gctggcggac gcgcatgcat ttcagaatct ccgcagggct cgaccactcc acgataccgc
     3121 cgcgaaaggc ttggcacgag ggccaatttc aatcaatttg accaggccaa ttcgacgcct
     3181 ctccgggcta agttgagcac atggagttcc acgtcagtct gatcggtcgc aaggacctga
     3241 gcggcgagat ataccgtcag ctgcggcagg cgatcctcga cggacggctt cggccggggg
     3301 ccaacctgcc tcccactcgc gagctggccc ggggacttca cgtctcgcgc accacggtga
     3361 ccgtcgccta cgatcggctg gatgccgaag ggttcgtgac gtcgagggtg ggtgccggga
     3421 cgttcgtgag ccacaccgcc ggtcgcgtga tgaaccaggg caaggctcat caagtggaag
     3481 gagcgttgcg gcccaagccc gtctgggaag cgatcccact gaccactccg tttgctcggc
     3541 ccgcgcgatt cgacttccgc accgggcttc cggatgcctc gctgtttcca cacgatcgct
     3601 ggcggcgcct gcttgcccga gagctacggt ccgaggaagt cgcggccgga gtgtatggcc
     3661 accctgccgg ccatcgaggc ttgcgcgagg ccatggcccg acacgtcggc atcgcgcggg
     3721 gcgtgaacgc gccggccgac gacatcatca tcacgagcgg catccagcag gccatcgaca
     3781 tcgtcgcgag ggtgttgctc cgcccaggcg accgcgtcgc aatcgaggat ccttgctatc
     3841 ctccgccgag acgcctgttg gagtcggtcg gtgcgaaggt ggtcggcgtg ccggtcgatg
     3901 atctcggact ggtagtcgat gccctgcccc ggcacacccg gctggtctac gtgacaccct
     3961 cgcatcagta tccgctcggc gtctccatgg cgctcccccg ccgtctggcg ctgctggcct
     4021 gggctgagcg gcacaatgcg gccatcatcg aggacgacta cgacagcgaa ttccgtttca
     4081 gcggccgccc gatcgagccg ctccagacgc tcgataccgc aggtcgagtc atctatctcg
     4141 gctccttttc caagaccatg ctgccgacgt tgcggctcgg cttcatggtc accccgcgat
     4201 cgcttcgcac tgccgtacag aaggcgaagt tcgtgaccga ctggcacacc gccctgccac
     4261 tccaggcagc attagcgcgc ttcatcgacg atggggaatt cgcccgacac gtgcggaagg
     4321 tcggcgcggt ctatcgaacc cggcacacaa ctctcaccgc tggtttggag aaggagcttg
     4381 gccagcatct ccagatcgtt ccgtgtgatg ccgggcttca tgtcgccgcc gttgcccgcg
     4441 tcgcctcggt cagggaagtc gctgaggcgg ctcggcgcgc gtccgagatg ggcgtcgagg
     4501 ttcaacagct ggcgacattc gcggtgaatc cgccgggaag agcgggcctc gtgctgggat
     4561 acggaaggat cgaggccggc cgcatggagg aagggcttcg gctgattcgg cagtgcttcg
     4621 agcagggagg aactctcgtc atcggatcgg gggtgcaatg aagctattgc gactcctacg
     4681 gtcagccacc cgtcagcgtg gcgagctccg ccctaacccg ctgtacccgc ggccgagcgg
     4741 cgggttccgg atcggcttgc aggatcaggt agtgacggta ggcgcggatg gcacctgccg
     4801 tatcgccggt caacgcggcc aggcgcccct cctcacgcaa atacgtcgag agaaagcgcg
     4861 gctccgccac gtcgtagagc cggcggcgcg tagcggccag tgcgcttgcg cgatcacccg
     4921 tcgcctccaa taatcgggct gtgaccaagt tccacgactc gttcatactc cgatagggag
     4981 ggccggtctt catgagcgaa tcaagcgcgc cgagcttccg tgctgcatcg ggtcgccgct
     5041 cggctccggc gagcatggcg tcgagtagga tggcacaccc gcgagcgatc atcacgaatt
     5101 ggctgtcgga ctgcgccggc tgaaacacgc ggagcgcagc gatggtacgc ggggcggtgg
     5161 caagctgacg gtttgccagg tgccattgct ccaggacgca ggcggcaagc ggcttcccga
     5221 cgtgattcgc cggaagggcg ccggcggttt gctccagtct tcgggccgat tcggcggcgg
     5281 ccagcgtgtc gccgtcccaa t
//
LOCUS       DASRJQ010000598         2421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1527843, whole genome shotgun sequence.
ACCESSION   DASRJQ010000598 DASRJQ010000000
VERSION     DASRJQ010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1527843"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..1054)
                     /locus_tag="VFB89_13595"
     CDS             complement(224..1054)
                     /locus_tag="VFB89_13595"
                     /inference="COORDINATES: protein motif:HMM:TIGR03843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HZM28375.1"
                     /translation="MTADDLGRLNEDGDARPLHPALTAELGQAELQITGRLLQASNAT
                     FLAELQGGGEPIECVYKPLEGERPLWDFPRRTLGLREVAAYEVSRIGGFDCVPVTVLA
                     DGPFGLGSLQVWVDVDEAATNQLVDLVPSSAVPEHGWFASVEGLDANDRPVSVIHADV
                     ADLRLLAVFDVLINNADRKGGHILSSAGRVFGVDHGVSFHTDHKLRTLLWGWAGAELN
                     LRELTAVRKARDEAPEQLDTLLSDREIAALVHRADLLLSRRRLPRPRGEWPSIPWPPF
                     "
     gene            complement(1063..1653)
                     /locus_tag="VFB89_13600"
     CDS             complement(1063..1653)
                     /locus_tag="VFB89_13600"
                     /inference="COORDINATES: protein motif:HMM:TIGR03847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 domain-containing protein"
                     /protein_id="HZM28376.1"
                     /translation="MAILVHRYDAPDRFVAGTVGQPGERTFFLQAREGNRITSVACEK
                     QQVSVLAEHLDRVLDEVVRRSTGDIDVPPASVKAHDTDPLDAPITEEFRVGTMTIAWD
                     PSIHRIIIELFSNVDVDEETAPESPASTTEESDEIDADEVIVVKLTPSYARDFVARAQ
                     ALVAAGRPSCPFCLQPMDAQGHICPRANGYRRPLFD"
     gene            complement(1729..>2421)
                     /locus_tag="VFB89_13605"
     CDS             complement(1729..>2421)
                     /locus_tag="VFB89_13605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_4193 family putative phosphomutase"
                     /protein_id="HZM28377.1"
                     /translation="TSANTAGVLAGRASGIGLDAAGAQQAADLASRLASVHLQAIVTS
                     PLRRCRQTAQALIAAQSDGCVYAVDRGLVECGYGEWTGRSLRELSKDKLWRAIQQQPS
                     AVRFPGGESMSEMSARAIAAVRAWNGRIEAEHGRDAVWAAVSHGDLIKAILADALGMH
                     LDSFQRIVVDPASISIIRYTAARPYLITANSTTVDLASFLSPPLKRSRSRPKSSKNAP
                     VGGGLGAADVSE"
BASE COUNT          444 a          781 c          784 g          412 t
ORIGIN      
        1 ctgatgagag gcgagccgac acgctgctcg tcggcgagtg acgacgaagt aggcggcctc
       61 cagcgcgatc tgcagcgagc ggtagcgagc aattcaaccc ggatcgcgcg ccgccgtgct
      121 gaggagaagc gagccgacac gcctgctcgt cggcgagtga cgacgaagta ggcggcttcc
      181 aggcgcgatc cgcggcgagc ggtagcgagc aattcaacac cgctcagaaa ggaggccatg
      241 ggatcgatgg ccattcgccg cgtggccgcg gcagccggcg tcgactgagc agcaggtctg
      301 cgcggtggac caacgctgcg atctctcgat cactgagcaa cgtatcgagc tgttcgggcg
      361 cctcgtcccg cgcttttcgt accgccgtca gttcccggag gttcagctcc gctccggccc
      421 agccccatag cagtgtcctc agtttgtgat cggtgtggaa gctcacacca tgatcaactc
      481 cgaacactcg cccggcgctg gagaggatgt ggcctccctt gcgatcagcg ttgttgatca
      541 agacatcgaa gacggcgagc agccggaggt cagcaacgtc ggcgtggatc accgatactg
      601 ggcgatcgtt cgcatcaaga ccttccaccg acgcaaacca gccgtgttcg ggtacggccg
      661 agctcggaac aagatcaacg agctggtttg tggctgcctc gtcgacatcg acccacacct
      721 gaagagatcc gagcccgaac ggcccgtcgg caagcaccgt gaccggcaca cagtcgaagc
      781 cgccgatccg ggacacctcg tacgcggcca cctctcggag gccgagggtg cggcgcggga
      841 agtcccagag tggacgctct ccctctagcg gcttgtagac gcactcgatg ggttcgccac
      901 ctccctgtag ctcggccaag aaggtggcgt tcgaggcttg cagcaggcgg ccggtgatct
      961 gcagctctgc ctgcccgagc tccgcggtca atgcgggatg gagcggccgc gcgtcaccgt
     1021 cctcgttcag gcgaccaaga tcatcggctg tcaaagcaag aatcagtcga acagcggccg
     1081 acggtagccg ttagcccgcg ggcaaatgtg cccctgcgca tccatcggct gcaggcagaa
     1141 cggacatgat ggccggccag cggccaccag ggcttgagcg cgggccacga agtcccgcgc
     1201 gtaggagggg gtgagcttga ccacgatgac ctcgtcggca tcgatctcgt cggactcctc
     1261 ggtggtggat gccggcgatt caggcgcagt ctcctcatcg acatcgacgt ttgagaacaa
     1321 ctcgatgatg atgcgatgaa tgctcggatc ccaggcgatg gtcattgtgc cgacgcggaa
     1381 ttcctcggtg atcggtgcat ccagtggatc cgtgtcgtgc gccttgaccg atgccggagg
     1441 tacgtcgatg tcgcctgtac ttcgccgtac cacctcgtca agcacccgat ccaggtgttc
     1501 ggccagcact gagacctgct gcttctcgca agccacgctg gtaatgcgat tcccctctcg
     1561 ggcctgcagg aaaaacgtcc gttctccggg ctggccaacc gtccctgcca cgaatcgatc
     1621 gggcgcgtcg tagcggtgca ccagaatcgc catgcgtaac agggtaggtc agttccgcac
     1681 tgtgctccta aagcattccg agggcggtcc ggccagcgac ccggagggct attcggagac
     1741 gtccgccgcc cccaggccac ctcccacagg cgcgttcttc gaggacttcg gtcgggagcg
     1801 tgatctcttg agcgggggcg acaggaagct ggccaggtcg actgtcgtgg aattggcggt
     1861 gatcaggtac gggcgggctg cggtgtagcg aatgatcgag atggacgccg gatcgacgac
     1921 aatccgctga aaactgtcca ggtgcatgcc aagcgcatcg gccaggattg ccttgatcag
     1981 gtcgccatga gaaaccgcgg cccagacggc gtcgcgcccg tgctcagcct cgatccgccc
     2041 gttccaggca cgcaccgcag cgatcgctcg cgcggacatc tccgacattg actccccgcc
     2101 gggaaaccgg acggctgagg gttgctgctg gatcgcgcgc cacagcttgt ctttgctgag
     2161 ctcccgcagt gacctgccag tccattcgcc gtacccgcat tcgaccagcc ctcggtcgac
     2221 ggcatacacg cagccgtccg actgtgcagc gatcagcgct tgagccgtct ggcggcagcg
     2281 acgtagtggt gaagtgacaa tggcctgaag atgcacactg gcgaggcggc tcgccaggtc
     2341 ggctgcctgc tgggcaccgg ccgcatcgag cccgatcccc gacgcgcgcc cggcaagaac
     2401 cccggctgtg ttcgccgagg t
//
LOCUS       DASRJQ010000599         3493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1788662, whole genome shotgun sequence.
ACCESSION   DASRJQ010000599 DASRJQ010000000
VERSION     DASRJQ010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1788662"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VFB89_13610"
     CDS             complement(<1..408)
                     /locus_tag="VFB89_13610"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZM28378.1"
                     /translation="MSGRVLVTGGAGFIGSHIAEGYLAAGWEVTVLDDLSRGAEKNVP
                     KCARLVRADIRSPEARRTLVEGRFDVLNHHAAQIDVRISVDRPALDADINLVGFVNLL
                     EGVAEGGVRRVVFASSGGVVYGETSVFPTPETDG"
     gene            complement(583..1836)
                     /locus_tag="VFB89_13615"
     CDS             complement(583..1836)
                     /locus_tag="VFB89_13615"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZM28379.1"
                     /translation="MSRMPRTALPRLSCRWPIGLAAGLALSLAPAAVRAQDQMPDTHT
                     VKKGDTLWDLAKQYLKDPFLWPEIYRLNTLVVEDPHWIYPGEVLRLAPSEKATAVPQA
                     DTTAVAASAADSGAAQPADSGAAQPAVAEMSADTMARAAEVVPVAQTVPVAPAPSDAE
                     ASETDGLFPPARGSMKIALTAGELPQRSQLRPTDFYASGFLTEGEMMPFGRLVGPVIP
                     LQIPTRDPRGNATLYTRVAVLPPTGATYQVGDSLLVVNAIGQIPGYGHIVEPRGMVRI
                     VSISEGTPIAEVIGVYGEITPDLSLLPAEKFVDLGTARPEAVSQGVEASVIGWPGRRE
                     LKGAGVVLFLDKGKRDGVSLGDVFEIRRAAGLRRDGMITVNDVMATVQVVHVRERTST
                     IRVVGVSSPDVPLGTHAVQVAKLPS"
     gene            complement(2071..2544)
                     /locus_tag="VFB89_13620"
     CDS             complement(2071..2544)
                     /locus_tag="VFB89_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZM28380.1"
                     /translation="MSRSLNKVILIGNLGADPEVRSTANGSRVATLSLATSRRWKNQA
                     GEQQEKTEWHRVILWNNKGSSLADVAERYCKKGDKIYIEGSIEYRSWQDREGQTRYTT
                     EITARELILLSGRGDAGENFSAPAKVAAGAAGKSKNESIDDFPEALDGEDDDLPF"
     gene            complement(2615..3145)
                     /gene="rimI"
                     /locus_tag="VFB89_13625"
     CDS             complement(2615..3145)
                     /gene="rimI"
                     /locus_tag="VFB89_13625"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HZM28381.1"
                     /translation="MAGLRKPRHAGNSLTDAPYRIRSGAPADAPTLAAIERRCFGDPW
                     SEASFRDALRASWSFGLVAEHADGIVGYLIAREAAGTGEILNLAVDQPWRRQGIAWAL
                     LDAGLVALQRRGTEEVYLEVRVSNDAARLLYHRAGFSPVGRRREYYRSPVEDALVLRR
                     ALDRSKNATDRVNLLD"
     gene            complement(3063..>3493)
                     /locus_tag="VFB89_13630"
     CDS             complement(3063..>3493)
                     /locus_tag="VFB89_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28382.1"
                     /translation="VRAAARGGAGATVLAVANALRGELYAAAYRFEPEAIETLLAPSV
                     WRVDSLIACAPRPDHFIGEGPEEALRRLEEWAKRHVVGGDEGLPRAALLLDLVRGSGG
                     ARPVTNFDLWEPEYGRPAEAQARWELAHGRPLPDSVGSTG"
BASE COUNT          623 a         1183 c         1087 g          600 t
ORIGIN      
        1 gccatcggtc tcaggggtcg ggaacacgct agtctcgccg tacactacac caccactcga
       61 cgcgaagacc acgcgtctca ctccaccttc ggccacacct tccagcaggt tcacaaagcc
      121 gaccaggttg atgtccgcgt cgagcgcggg ccgatccacc gagatccgga cgtcgatctg
      181 tgccgcgtga tggttgagca cgtcgaaacg tccctcgacc agcgttcgcc tcgcctcggg
      241 cgagcggatg tcggcccgca ccaggcgggc acacttcggg acgttttttt cggcaccgcg
      301 ggagaggtca tccaggaccg tcacctccca cccagccgca aggtagccct cggctatgtg
      361 ggatccgatg aagccggcac cgccggtgac caggactcga ccgctcatcc ctgcacgtac
      421 tccctccggg cccttcggcc gtcattacgt ccgggactca ccgctgaatt gccccgcaat
      481 atagaaagcg tacgcgcgga cggaaccgcg cacgtgaggc ttcctaatgc acgcggcgcc
      541 gagcccacgt gaactcggcg ccgcagagag tgtgcgggtg acttacgagg ggagcttggc
      601 cacctgaaca gcgtgcgtcc caaggggcac gtccggtgag gagacgccta cgacccgaat
      661 ggtcgacgtc cgctcgcgga catggaccac ctgcacggtg gccatgacgt cgttgacggt
      721 gatcatcccg tcccggcgta gacccgcggc gcggcgaatc tcaaagacgt cgcccagcga
      781 gacgccgtcg cgcttgcctt tgtcgaggaa cagaaccacc ccggcgccct tcagctcgcg
      841 acgacccggc catccgatga cactcgcctc aactccctgg gataccgcct ccgggcgagc
      901 cgtaccaagg tcgacgaact tctccgctgg caggagagag agatcgggag tgatttcacc
      961 gtacactccg atgacctcgg cgatcggagt gccttcggag atggagacga tgcgcaccat
     1021 tccgcggggc tccacgatat gtccatatcc cgggatctgg cctattgcgt tcacgaccag
     1081 aagcgaatcg cccacctgat acgtggcgcc cgtgggcggg agcaccgcga cacgagtgta
     1141 cagcgtggcg ttgccgcgcg gatcccgagt cgggatctga aggggtatca ccggcccgac
     1201 cagccggcca aacggcatca tctcaccctc ggtgagaaaa cccgaggcat aaaaatcagt
     1261 tggccggagc tggctccgct gcgggagctc gccggcggtc agggcgattt tcatcgagcc
     1321 gcgggccggc ggaaagaggc catccgtttc ggaggcttcc gcgtcgctag gcgctggggc
     1381 gacggggaca gtctgggcga ctggcacgac ttcggccgca cgtgccatcg tgtcggcgct
     1441 catttcggca acggcgggct gtgcggcccc gctgtcagcc ggttgtgcag ctccgctgtc
     1501 ggcagccgaa gcagcgacgg cggtggtgtc cgcctgcgga acggcggtgg ccttctcgga
     1561 tggagccagc cgcaggacct cacccggata gatccagtgc gggtcttcca ccaccagcgt
     1621 gttcaggcgg tagatctcgg gccagagaaa cgggtccttg aggtactgct tggccagatc
     1681 ccacagcgtg tcgcctttct tcaccgtgtg cgtgtccggc atctggtcct gcgcgcgtac
     1741 cgctgccggt gcaagtgaga gagccaggcc ggcggccagg ccgatcggcc aacggcacga
     1801 gagtcgaggc agcgcagtcc gaggcatgcg ggacatcagg gtctcccggc gacaggggca
     1861 agcgggttca tattgggaaa aacgaggcga ccgcggggcc gcaactcgcc tcgcggtcgc
     1921 aagatcagcg ccaccctacc gccctgcaga ccctgggccc taattccttg ccgaaccgcc
     1981 gcttagagca ccagaggcgg gcgatttcgg gaccccggcc ctagcgttca gcgccaactt
     2041 tcgcattatc gcgcccggct tctggcgcgc tcagaaaggc aggtcgtcgt cctctccgtc
     2101 caacgcctcg gggaaatcat cgatcgactc gttcttgctc ttgccggccg ctcccgcggc
     2161 gaccttggcc ggcgccgaga agttctctcc ggcatcgccc cgtcccgaca gcagaatcaa
     2221 ctcacgcgca gtgatctcag tggtgtagcg agtctgcccc tcacgatcct gccacgagcg
     2281 atactcgatg ctgccctcga tgtagatctt gtcgcccttc ttgcaatacc gctcggcgac
     2341 atcggctagg ctggacccct tgttgttcca cagaatcacg cggtgccact cggttttctc
     2401 ctgctgttca cctgcctgat ttttccagcg ccggctggtg gcgagggaca gagtggccac
     2461 ccgggagccg ttggcagtgc tccgcacttc cgggtcggct ccgagatttc cgatcagaat
     2521 gaccttgttc aagctacggc tcacagatgc ctccgcggtt ccaaggggtc caatgtaagg
     2581 tggacccggc taaagtatag gactcgcaag ttgttcagtc aagcaaattt acgcggtcag
     2641 tggcgttttt ggatcggtcg agggcccgac gtagtacgag ggcatcttcg accggactcc
     2701 gatagtactc gcgtctccgt cctaccgggc tgaacccggc ccgatgatag agtagccggg
     2761 cggcgtcatt ggacacgcga acttcgaggt agacctcttc agtgccgcgt cgctgcaaag
     2821 cgaccaggcc cgcgtcgagc aacgcccacg caattccttg ccgacgccac ggctgatcga
     2881 cagccaggtt cagaatttcc ccggttcccg ctgcctcgcg cgcgatcaag taccccacga
     2941 tgccgtcggc atgctccgcc acgagaccga agctccagct tgcacggagt gcatcgcgaa
     3001 agcttgcctc gctccaggga tcgccaaagc aacgccgctc gatcgcggcg agcgtcggcg
     3061 cgtcagccgg tgctcccgac cgaatccggt aaggggcgtc cgtgagcgag ttcccagcgt
     3121 gcctgggctt ccgcaggccg gccatactcg ggctcccaca gatcgaagtt tgtcaccggg
     3181 cgggcaccac cggacccgcg caccaaatcg agcagaagcg cggcgcgagg aagtccctcg
     3241 tctccgccaa ccacgtggcg tttggcccat tcctccaggc ggcgcagcgc ctcctcgggt
     3301 ccctctccaa tgaagtggtc ggggcgaggt gcgcaggcaa tcaggctgtc aactcgccag
     3361 accgatggag cgagtagtgt ctcgattgct tcaggttcga agcgataggc tgccgcatag
     3421 agctcaccac gcagagcatt cgcgacggcc agcaccgtcg caccggctcc gccacgcgcg
     3481 gccgcccgca cca
//
LOCUS       DASRJQ010000600         2092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1508113, whole genome shotgun sequence.
ACCESSION   DASRJQ010000600 DASRJQ010000000
VERSION     DASRJQ010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508113"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..932)
                     /locus_tag="VFB89_13635"
     CDS             complement(3..932)
                     /locus_tag="VFB89_13635"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZM28383.1"
                     /translation="MTYLAALLFGVTVAFAVWLVARMVPTRPRAVARQLAELERMSGS
                     ADEAVRRERITRSGQLKALLEFLGGRFGGDAKRRAQLRELMIHAGYREAGAVPIYWGA
                     RVALLIGLGLGFLVLAKALGAEAPRVIVAGFAGAALGWVWPSVHVGSRARKRQKEIQR
                     TLPDALDLMIVCVEAGLALNQALVRVAEEIRHVSRLTNQELAQVNYEIRAGVPREEAL
                     RNLGERTGVADVRSLVAMLIQTDRFGTSIAQALRVHSDTLRTKRRQRAEEAAAKTTIK
                     LVFPLALCIFPALFVVILGPALVQIYRTMIGLK"
     gene            complement(943..1911)
                     /locus_tag="VFB89_13640"
     CDS             complement(943..1911)
                     /locus_tag="VFB89_13640"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZM28384.1"
                     /translation="MVGPALLVFITVGLATVALALAIQWVFEFRERRAVVRQLQDYGT
                     ATVASATSPALMRDAAASEAKWLGAITARLPRVPGLGRILEQGGLTWSPQSFAAVTLG
                     LSLGAGLTMLVVFPVLPAVLIAAAVAGAMPYFYIRRKQRQRLESFEEQLPEAIDLIGR
                     AMRAGHPLSAGLKMASEESQEPVAGEFRRVFEEQRFGMPFDEAVLGLTDRVALIDVRI
                     LVTAILVQRDVGGNLAEVLDKIAYVIRERFMIKRQLRVYTAQGRFSGIVLALLPIAVG
                     TALFFINREYTLTLFLDPIARYFLVLAVILQILGYFWIRKIVDIEI"
     gene            complement(1905..>2092)
                     /locus_tag="VFB89_13645"
     CDS             complement(1905..>2092)
                     /locus_tag="VFB89_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28385.1"
                     /translation="MQDIFTFERQGVGEDGRVLGRFRATGLRPRFTERLQAWGIELPA
                     GVFTPMAERTAAEGARW"
BASE COUNT          395 a          724 c          656 g          317 t
ORIGIN      
        1 gctcatttga ggccgatcat ggtacggtag atctgtacca gcgcgggccc gaggatcacc
       61 acgaacaggg cagggaaaat gcagagggcg agcgggaaga cgagcttgat tgtcgtcttg
      121 gccgctgctt cctccgcgcg ctgccggcgc ttggtccgca gcgtatcgga gtgcacgcgc
      181 agcgcctgcg cgatggacgt cccgaagcga tcggtttgaa tcagcatcgc gaccaaggac
      241 cgcacgtcgg ctactcccgt ccgctccccg aggttgcgca gcgcttcttc ccggggcacg
      301 ccggcacgga tttcataatt gacctgtgcc agctcctggt tcgtcagccg gcttacgtgc
      361 cggatttctt cggccacccg caccagcgcc tggttcaagg cgagtcccgc ttcgacgcaa
      421 acgatcatca gatcgagcgc gtcgggcaga gtgcgctgaa tctccttctg ccgttttctg
      481 gcccgggagc cgacgtgcac cgagggccag acccacccca acgccgcgcc cgcaaaaccg
      541 gcgacaatca cacggggggc ttcggcgccc aaggctttcg ccagcaccag gaaccccagc
      601 cccaatccga tgagcaaggc aacccgtgcg ccccagtaga tcgggacggc accggcttcg
      661 cggtacccgg cgtgtatcat gagctcgcgc agctgcgcgc gacgcttggc atctccaccg
      721 aagcgcccgc cgaggaattc cagcagcgcc ttgagctggc cggaacgggt gatccgttca
      781 cgccggacag cttcgtccgc cgatccgctc atgcgctcga gctcggcgag ctggcgcgcc
      841 acggcgcggg ggcgggtagg gaccatgcgg gccaccaacc acaccgcgaa ggccaccgtc
      901 actccgaaca gcaatgccgc caaataggtc acaggatgct cctcagattt cgatgtccac
      961 gatcttgcgg atccagaagt agcccaggat ctgcaggatc acggcgagca ccaggaagta
     1021 tctggcgatc gggtccagga acaacgtgag cgtgtactcg cggttgatga agaacagtgc
     1081 cgtgccgacc gcaatcggca gcaaggccag gacgatgccg ctgaatcgtc cctgagcggt
     1141 gtagacgcgc agctgtcgct tgatcatgaa acgttcgcgg atcacgtagg cgattttgtc
     1201 cagcacctcg gcgaggttgc cgccgacgtc acgctgcacc aggatcgccg tcaccaggat
     1261 ccgcacgtcg atgagcgcga cgcgatccgt gaggccgagc accgcctcgt caaagggcat
     1321 cccgaagcgc tgctcctcga agacacggcg gaactcgccg gccaccggct cctgtgattc
     1381 ctcgctggcc attttgaggc cagccgacag gggatgaccg gcccgcatgg cccggccgat
     1441 cagatcgatc gcctcgggga gctgttcctc gaacgactcg agccgttgcc gttgcttgcg
     1501 cctgatgtag aagtagggca tcgccccggc aacagccgcc gcgatcaaca cagccggcaa
     1561 caccgggaaa acgaccagca tcgtcaaccc ggcaccgagg ctcaagccca gtgtgacggc
     1621 cgcgaagctc tgaggcgacc aggtgagacc gccctgctcg aggatacgtc ccaagccggg
     1681 cacgcggggg agtcgtgcgg tgatcgcacc cagccacttg gcttccgacg cggccgcatc
     1741 gcgcatcagc gcgggcgagg tcgcactggc caccgtcgct gtgccataat cctgcagctg
     1801 acgcacgacc gcacgccgtt cccggaactc gaatacccac tggatcgcga gcgccaaggc
     1861 cacggtggcg agcccgaccg tgatgaagac gaggagcgcc ggccctacca tcgggcacct
     1921 tccgcggcag tccgctcggc catcggggtg aacaccccgg ccggcagctc gattccccac
     1981 gcctgaagac gttcggtaaa ccgcggacgc aggccggtcg cccggaagcg ccccagcacc
     2041 cgcccgtctt cgcccacgcc ctgacgctcg aaggtgaaga tgtcctgcat cg
//
LOCUS       DASRJQ010000601         4803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1077594, whole genome shotgun sequence.
ACCESSION   DASRJQ010000601 DASRJQ010000000
VERSION     DASRJQ010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077594"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VFB89_13650"
     CDS             complement(<1..279)
                     /locus_tag="VFB89_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28386.1"
                     /translation="MVTVVPQFAALALRAFAALHPMHTSVAELAPAGTSVVVTIRVFA
                     DDFRTAVPDGAAQKAEPYIRSRFELRDPENRPIAVRWEEIARSGDVFQM"
     gene            complement(273..872)
                     /locus_tag="VFB89_13655"
     CDS             complement(273..872)
                     /locus_tag="VFB89_13655"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HZM28387.1"
                     /translation="MSDLATFAKLGFHHIVNPAALDHILFLLALAAVYRREDWRDALW
                     VVTAFTVGHSITLALAVTGALVLPTPLIEFLIPVTIVVTALENIAVRSRAVTPWNGRY
                     RAVFTAVFGLVHGAGFANYLRTLFDGPIVVPLFGFNLGIELGQILVLSLAAVLLAVID
                     RLLSLRRGDLLTAYRWRVVSVSAAVLLVASRMAVERRPW"
     gene            complement(869..2158)
                     /locus_tag="VFB89_13660"
     CDS             complement(869..2158)
                     /locus_tag="VFB89_13660"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM28388.1"
                     /translation="MIRRLLISIVTLTGVAATAVTLKGVTRAAAPAVPALSEAEVRDR
                     DIAFYQARIERDPLGARDRAQLAGLFLDRARDTGDNADLVRAEETARGSLANRRAHNS
                     KALAVLINSLLGQHRYPEALAAARDLAQLEPEGRSVRAQLGEIEMELGQYDSARAVFD
                     SLAGWTRDLAVAPRLARWLELQGRNEEAHRLLVQARDNARRLPGLSGEQAAWFEFRVG
                     EVALRNGRLGEAEKAFRAGLEAHPEDYRLLAAMARVAVARHDWRAGIDWGDRAIARHL
                     DPATLALVGDAYRALGNSAKSEEYYRVLEVSLGGQTGAFHRAWSLALLDRGRRVPELL
                     EKAQEEIKVRQDVYGWDLLAWALHQAGRDHEASAAMTHALSLGTRDAMLFYHAAMIEN
                     AVGHRETARAWLQRALTVNPYWHPSQPAEARAALGVS"
     gene            complement(2170..2769)
                     /locus_tag="VFB89_13665"
     CDS             complement(2170..2769)
                     /locus_tag="VFB89_13665"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HZM28389.1"
                     /translation="MTFATLVRGLGTRALCLAAALALVAACSDDGTMTTPTDPANMRS
                     YDQVQRLGNPLVSEVLLQKADHPLHGSIGPDQDLALVAPRALAFITGPGSVAGRSADY
                     ANVLGSVLLPDMLIVQTDKPANTAGWLNWVGVAPLANGYGGRKLTDDVVDLALLAIFG
                     DPFGLDPAGAQGKQALTTDNVAFDSPGITTTFPYLAPPN"
     gene            complement(2800..3543)
                     /locus_tag="VFB89_13670"
     CDS             complement(2800..3543)
                     /locus_tag="VFB89_13670"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HZM28390.1"
                     /translation="MNRRFMTRGRAAIAGLVVLAGAAALVRVAVGSDHQDTPLVELNP
                     TQDMTDVYAFPGSAAGRIALVLNSRAFLTPAQTAGASFDNSLLYQFKIDNNGDAKEDK
                     VIQVTFTGTGANQTVEVRGPLDPPVQGAMGNKVSTTAPTVSGKINTVLGTSSGMQVFA
                     GPRDDPFFIDLEAAFCILPDRKPVTGPLAGNCALTPNPSAPFFFRPAGQAVNYVKGFN
                     VLSIVVELPSSMIEKGAPGKLGIWGTISR"
     gene            3803..>4803
                     /locus_tag="VFB89_13675"
     CDS             3803..>4803
                     /locus_tag="VFB89_13675"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZM28391.1"
                     /translation="MHTIDGTPLAEAVVAPLGGPAAGSVTTASAGRFKLRVPAGTSQL
                     VAAHIGFAPETLAVSAEMLFRLRPAPVTLDPLVVSADPAFSAASSRVIRSLDIELRPR
                     ESSQELLRLTPGLVIAQHAGGGKAEQIFLRGFDADHGTDVAISVDGAPVNMVSHAHGQ
                     GYADLHFLIPEVVERVDVRKGPYDAADGDFATAGAMSFRTKDRISGAEVETRGGSFGT
                     VHGVSLIPFGGDAAHAGGYVALSAAYSRGPFLASQDHRRLNAFARWTAPVGSGVTLAA
                     TASGFDARWNASGQIPGRAVARGLVDRFGSLDPTEGGATYRYDASVALRSGTGGGGW"
BASE COUNT          815 a         1555 c         1616 g          817 t
ORIGIN      
        1 catctggaag acgtcgccgg agcgggcgat ctcctcccat cgaaccgcaa tcggacggtt
       61 ctccgggtca cgcagctcga agcgggagcg gatgtacggc tccgccttct gcgctgcacc
      121 gtcgggcacc gcggtgcgga aatcgtctgc gaacactcga atcgtcacca caacggaggt
      181 gccggccggg gccagttcgg caacggacgt gtgcatgggg tgtagcgcag cgaacgcccg
      241 gagggccaga gcagcaaact gtggcacgac ggttaccacg gccgccgctc cactgccatg
      301 cgactcgcca ccagcaggac cgcagcagac acgctcacga ctcgccagcg gtacgctgtg
      361 agaagatcgc cacggcgcaa gctgagcagt cggtcgatga cggccagcag gacggcagca
      421 agcgaaagca ccaggatctg cccaagctcg atcccgaggt tgaacccgaa cagcggaacg
      481 acgatggggc cgtcgaacag ggtgcggagg taattggcaa acccggcgcc gtgaaccagc
      541 ccgaagactg ccgtgaacac cgcacgataa cggccgttcc atggggtgac cgcccggctt
      601 cgcaccgcga tgttctcgag cgcggtaacg acgatggtga cgggaatcag gaactcgatg
      661 agcggtgtcg gcagcacgag tgctcccgtc accgcgagcg ccagcgtgat cgaatgaccc
      721 acggtaaatg cagtcaccac ccagagggca tcgcgccagt cttcccggcg gtacaccgcc
      781 gcgagcgcaa gcagaaacag gatgtggtcc agcgcagccg ggttgacaat gtggtggaag
      841 ccaagcttgg cgaaggtggc caggtcgctc atgagacccc cagcgcagca cgggcttccg
      901 ccggttgact gggatgccag tacggattca cagtgagcgc gcgttgcagc caggcccggg
      961 cggtttcacg gtgaccgaca gcgttctcga tcatggccgc gtgatagaac agcatcgcgt
     1021 ctcgggtgcc gagcgagagc gcatgcgtca ttgcggcgga ggcttcatgg tcgcgccccg
     1081 cctgatgcag cgcccacgcc agcaagtccc agccgtagac gtcctgccgc actttgatct
     1141 cttcttgagc cttctccagc aactccggga cgcgacggcc ccgatccaga agagcaagac
     1201 tccaggctcg gtgaaacgcg ccggtctgac cgccgagcga cacctcgagc acccgatagt
     1261 actcctccga cttcgcggag ttgcccagcg ccctgtatgc gtcaccaacc agggccagcg
     1321 tggcgggatc gagatggcgc gctatggccc ggtcacccca gtcgatgccg gcccgccagt
     1381 cgtggcgagc gacggcaacg cgcgccattg ccgcgagcag ccggtagtcc tcaggatgag
     1441 cctcgaggcc ggcgcggaat gctttctcgg cttctccgag tcgaccattt cgcagcgcga
     1501 cttcgccaac cctgaactcg aaccaggcag cttgctcgcc ggagaggccc ggcagacgcc
     1561 gggcgttgtc tcgcgcctgc accagcagcc ggtgggcctc ttcgttacgg ccttgcagct
     1621 cgagccagcg cgccaagcga ggcgcgactg cgaggtccct ggtccaaccg gcgagcgagt
     1681 cgaagaccgc gcgggcggag tcgtactgcc ccagttccat ctcgatctcg cccagctgcg
     1741 cgcggaccga gcgtccctcc ggctccagct gcgccagatc gcgtgcggcg gcgagcgcct
     1801 cgggatagcg gtgctggccc agcaggctgt tgatcagcac cgcgagggcc ttgctgttgt
     1861 gagcgcggcg gttggccagc gagccgcggg cggtttcttc ggcgcgaacc aggtcggcgt
     1921 tgtcgccggt gtcgcgcgcg cgatccagaa agagtcccgc caattgagcc cgatcccgtg
     1981 cacccagcgg atcgcgctcg atccgtgcct ggtagaaggc gatgtcacga tcccgcacct
     2041 cggcctcact gagagcgggc acggcgggag cggcggcccg ggtcactcct ttgagggtga
     2101 ccgcagtcgc ggccaccccc gtaagagtga cgatcgaaat cagaagtcgt cttatcatga
     2161 cggtccgcct cagttgggcg gcgcgaggta ggggaacgtc gtcgtgatgc ccggcgagtc
     2221 gaacgccacg ttgtcggttg tgagcgcctg cttaccctgg gcgccggccg gatcgagccc
     2281 gaagggatcg ccgaagattg ccaggagtgc caggtcgacg acgtcgtccg tcagcttgcg
     2341 accgccgtat ccatttgcca gtggcgccac acctacccag ttgagccatc ccgccgtgtt
     2401 cgccggcttg tcggtctgga caatcagcat gtccggaagg agcacgctgc ccagcacgtt
     2461 ggcgtaatcg gcggaccggc cggcgacgct gccgggtcca gtgatgaagg cgagagcgcg
     2521 cggagcaacc agcgccagat cctgatccgg cccgatggag ccatgcagcg ggtgatccgc
     2581 cttttgcagc agcacctcgc tgaccagcgg attccccagc cgctgcacct ggtcgtacga
     2641 ccgcatgttc gcgggatcgg tcggcgtcgt catcgtgccg tcatcgctgc aggcggcaac
     2701 gagcgcgagt gcggctgcca ggcagagtgc tctggtgccg agcccgcgga cgagagtcgc
     2761 gaaagtcatg gtatgagtcc tcgagtggtg tgcaatgggt tatcggctga tagtgcccca
     2821 gatgcccagc ttacccggcg cgcccttttc gatcatcgag ctgggcagct cgacgacgat
     2881 cgacagcacg ttgaatccct tcacgtagtt caccgcctga cctgcggggc ggaagaagaa
     2941 cggggctgac gggttcggtg tgagagcgca gttgcccgcg agcgggccgg tgacgggctt
     3001 cctatctgga aggatacaga atgcggcctc gaggtcgatg aagaagggat cgtcacgagg
     3061 gccggcgaac acctgcatgc cggacgaggt tccgagcacc gtgttgatct tgcccgagac
     3121 agtcggggcc gtagtggaca ccttgttgcc catggctccc tgaactggcg gatccagcgg
     3181 gccacgcact tccacggtct gattggcgcc ggtgccggtg aaggtcacct ggatgacctt
     3241 gtcttccttg gcatcgccgt tgttgtcgat cttgaactgg tagagcaggc tgttgtcgaa
     3301 cgaggcgccg gccgtctgag ccggagtgag gaacgcgcgg ctgttcagga ccagtgcgat
     3361 ccggccggcc gccgagccgg gaaatgcgta cacgtcggtc atgtcctgcg tgggattgag
     3421 ctcgaccagc ggcgtgtcct ggtggtctga ccccacggcg acgcgcacca gggcggcggc
     3481 gcccgccagg acgacgagtc ccgcgatcgc ggctcggccc cgcgtcatga atctgcggtt
     3541 catcggtgtg tctctcctta aatgcggatg gacgggagat gctgcatctg gttcccaaca
     3601 tcccttctac gccacccgct cgacgatcgg attgcgacct cccggaaatc cgattgcgcg
     3661 tcgtcccgcg tagagcttcc gactccagcg gaagccgatt ctgtttccct cacgtgcggg
     3721 ccttgcttcg gggatgtgtc ttgggctttt gctgaccggg tcggtcggtc gcgcgcagga
     3781 tgcccgttcg attagcggaa gcgtgcatac catcgacggc actcccctcg ctgaggcggt
     3841 ggttgcgccg ctcggcggac cagccgccgg ctcggtcacc acggcgagcg cagggcgatt
     3901 caagctccgc gtgcctgccg gaaccagtca gctggttgcg gcgcacatcg gattcgctcc
     3961 tgagacgctg gccgtgtcgg ccgagatgct atttcggctc cgtcctgcgc cggtgacgct
     4021 cgatccgctg gtggtctccg cggacccggc gttttcagcg gcatcttccc gcgtcatccg
     4081 ttcgctcgat atcgagcttc ggccccgcga gtcttctcag gagttgcttc ggctcacacc
     4141 cggactggtg attgcacagc atgcgggtgg cggaaaggcg gagcagattt ttctacgcgg
     4201 attcgacgcg gatcacggga ccgacgtcgc gatctcggtg gatggcgctc cggtcaacat
     4261 ggtgtcacac gcccatgggc aaggttatgc cgacctccac ttcctgattc cggaggtcgt
     4321 cgagcgggtg gacgtgcgaa aggggcccta tgatgccgcc gacggtgact tcgcgacggc
     4381 aggcgccatg agcttccgta ctaaggatcg aatcagcggt gctgaagtcg aaacccgagg
     4441 cggaagcttc ggcaccgttc atggcgtgag cctcattcca ttcgggggcg acgcagccca
     4501 cgccggcggt tacgtcgcgc tctcggccgc gtactcccgc gggccgtttc tggcttccca
     4561 ggaccaccgg cggctcaatg catttgccag gtggactgcc cccgttggat ctggcgtaac
     4621 gctcgcagca accgcatccg gcttcgacgc ccggtggaac gcctcgggcc agatccccgg
     4681 gcgggccgtc gctcgaggtc tggtcgaccg attcggctcg ctcgatccaa ccgaaggcgg
     4741 tgccacgtac cgctacgatg cgagtgtggc actgcggagc ggcaccggcg gaggcggctg
     4801 gga
//
LOCUS       DASRJQ010000602         2279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_140104, whole genome shotgun sequence.
ACCESSION   DASRJQ010000602 DASRJQ010000000
VERSION     DASRJQ010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140104"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1679)
                     /locus_tag="VFB89_13680"
     CDS             complement(<1..1679)
                     /locus_tag="VFB89_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28392.1"
                     /translation="MNRLTRLAPLLVFTIACAVFAQTVGYGFAGDDLSGIRDRVLFHD
                     LKNWQEILTSSWWPHALYRPLTGLSLALNWVLAGGHPGVFHAVNLILHGIASVLVYRL
                     ARELFGQAAALGAGLLFAVHPVHVEPVVNLVGRAEVLSALFGLGAVLFYRWDGRLADA
                     AELGSWRRLTATLGTLACIVLALGAKESAFAVPGLLLLMDWYEAAVGNRRIGDVIRRH
                     SALWVGALAVCVGWLFLRSRIVQDLTGLEAAPGLEFQGLVGRAVIMLPMTLEYVRLLF
                     VPARLSVEYSPNFVPVSTELTVRGMAGAALVIGAVTLAVVTRRRAPVIGLAVGWLAAT
                     LIIICNVLVPTGVLVAERTLYLASVGAVLLLGWVFGEVFRRAPSAAIAGLWVAVAAGG
                     LRTVTRNPIWRDNDTYMRAMVSDAPGSFRAEWTEAEFARREGDLRGAEAHLRRAVQIH
                     PLGWHVWRNLATLMYLEGRYPEAAQYFSAAWRINGGSAVDAQRAIQSHLQAGRLDSAA
                     TFLEAAVSKRPNMPVELTLAASDIAVARGDFLQAMTLRRRAAIDFPDSARY"
     gene            complement(1924..>2279)
                     /locus_tag="VFB89_13685"
     CDS             complement(1924..>2279)
                     /locus_tag="VFB89_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28393.1"
                     /translation="LTAQRSIIAALRVGQVDTAVARLAIARAARPQSWELWLAGGEVA
                     MAQGQPLRAMTLRRQAAWQFPDTARFWGLTAEAALKARQCPELVVAIQHLRALKAESA
                     GVAPLEAGARGLGCR"
BASE COUNT          461 a          754 c          745 g          319 t
ORIGIN      
        1 caatagcggg cggaatcggg gaagtcgatg gccgcgcgcc tcctcaaggt catcgcctgg
       61 agaaaatctc ctcgagccac tgcgatgtcc gatgcagcca gggtcagctc gaccggcatg
      121 ttcgggcgct ttgatacggc agcttccaga aaggtggcgg cgctatcgag ccggccggcc
      181 tggaggtggg actggatggc gcgctgggcg tctacagctg atccgccgtt gattcgccag
      241 gccgccgaga aatactgcgc ggcttcggga tagcggcctt cgagatacat cagcgttgcc
      301 aggtttcgcc agacgtgcca acccagcgga tggatctgaa ccgcccggcg cagatgagct
      361 tcggctcccc gcaggtcacc ctcccgcctg gcgaactccg cttcggtcca ctccgcccgg
      421 aatgatcccg gagcatcgct gaccatcgca cgcatgtagg tgtcgttatc ccgccagatc
      481 ggattccgcg ttacggtccg aaggccgcca gccgccacgg caacccacag gccggcaatc
      541 gcagcgcttg gcgctcgacg gaatacctcg ccgaaaaccc agcccaacag aaggactgcg
      601 cccactgaag ccagatacag ggtgcgctcc gccacgagaa caccggtcgg cacgagcacg
      661 ttgcagatga tgatcagtgt ggccgccagc cacccaactg cgagcccgat cacgggcgcc
      721 cgtcggcgag ttaccactgc cagcgtcacg gcgccgatca caagcgcggc gccggccatg
      781 ccccgaacag tgagctccgt cgaaaccggc acgaaattgg gcgaatactc caccgacagc
      841 cgggcgggaa cgaacaggag gcggacgtac tcgagcgtca tcggcaacat gatcacagcc
      901 cgcccgacca gcccctgaaa ctcgaggccg ggcgccgctt ccaacccggt caggtcctgg
      961 acgatgcggg agcgcaggaa gagccagccc acgcagactg caagagcacc gacccataga
     1021 gcggagtgac gcctgatgac gtcaccgatc cgtcgattcc cgaccgctgc ctcgtaccag
     1081 tccatcaaga ggagcaagcc ggggaccgcg aaggcggatt ccttggctcc cagggccagc
     1141 acgatgcacg cgagggttcc cagagtcgcg gtgagtcgcc gccaggaacc cagctcagct
     1201 gcgtcggcga gtcgcccgtc ccatcgatag aacaacacgg caccaagccc aaagagagcc
     1261 gacagcactt cggcccggcc gaccaggttc accacgggct cgacatgaac tggatggact
     1321 gcgaagagaa gtccggctcc aagggcggct gcctgcccga acagctcgcg agcaagccgg
     1381 tagaccagca cgctcgcaat cccatgaaga atcaggttca ccgcgtggaa cactccggga
     1441 tgtccgccgg cgagaaccca attcaaggca agcgacagcc ccgtgagtgg ccggtacaag
     1501 gcgtggggcc accacgagct cgtgagaatt tcctgccagt tcttcagatc gtgaaagagc
     1561 acacgatcgc ggattccgga gagatcatca ccggcaaagc cgtaccccac ggtctgggcg
     1621 aagacggcgc aagcgatcgt gaacaccagc aggggggcga gccgcgtgag ccggttcata
     1681 ggagggggat gttgccgcag ctcgcggcac cccacgcggc gttgcggaag gcggcggatg
     1741 ggatacgcgc gctgggcaaa gcaggatagc gagccggatg cggcttggag actttcatgg
     1801 aagcgattgc ggttccagta gttgctggca tgggtcagga atgccggccc tccgtccaac
     1861 catgttggag tgggcggcgg gaccaaactg gagcgcaggt atttgcatgc tccgcgccag
     1921 cgcctagcgg caccccaggc cgcgggcacc cgcttcgagc ggagcgactc cagcggattc
     1981 ggccttgagc gcccgcagat gttggattgc gacgacaagc tccgggcact gccgggcctt
     2041 gagtgctgcc tcggcggtga gtccccagaa gcgtgcggtg tcggggaact gccaggccgc
     2101 ctgccgccgc agggtcatgg cgcgaagcgg ttggccttgc gccatggcga cctcaccacc
     2161 ggcaagccag agctcccagc tctgcggccg tgcagcccgg gcaatcgcga gccgagccac
     2221 cgcggtatcg acctgaccca cccggagggc ggcgatgatc gatcgctgcg ccgtgagca
//
LOCUS       DASRJQ010000603         2699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_564228, whole genome shotgun sequence.
ACCESSION   DASRJQ010000603 DASRJQ010000000
VERSION     DASRJQ010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564228"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..302
                     /locus_tag="VFB89_13690"
     CDS             114..302
                     /locus_tag="VFB89_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28394.1"
                     /translation="MGITDKVTGKAKQVAGDVSGDSSLRRKGRNEEKKGEKKDELHRA
                     DERANEKADEVADLERKT"
     gene            647..1006
                     /locus_tag="VFB89_13695"
     CDS             647..1006
                     /locus_tag="VFB89_13695"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZM28395.1"
                     /translation="MGITLELEPGPEAAHQARDEVRYHLRDRVENGLLTDLQLIVSEL
                     VTNSVRYGRGETIEVEIAVAEDGSIIGEIEDHGRGEVAMREITDQGGGFGLRIIDALA
                     SRWGVCEGSTQVWFELS"
     gene            1057..1392
                     /locus_tag="VFB89_13700"
     CDS             1057..1392
                     /locus_tag="VFB89_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28396.1"
                     /translation="MPGERERRLGKNETLFRTANERMAGWEERDRLEAVELYFCECAD
                     PTCMKKARLCGSDYERVRSDPTHFFVVPGHEVADVETVLESHPDWVLVEKAESEARDV
                     AEETDPRQD"
     gene            complement(1453..1962)
                     /locus_tag="VFB89_13705"
     CDS             complement(1453..1962)
                     /locus_tag="VFB89_13705"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZM28397.1"
                     /translation="MPYDDKGGPLPSISTVLLAVSGSGPLCLSLRARDTAQAMSQENV
                     ELARRGFEAWEQGDLETHLSLIHEDVTCRQVGQIDAETYHGLEGYIEFASQWLEPYEE
                     LEFRPSEFMDAGDKVVVEVPQEGRLVGSDQPITGTFWFVITARDGKMAHLEIYNERDP
                     ALEAAGLRE"
BASE COUNT          513 a          841 c          894 g          451 t
ORIGIN      
        1 gtgcgaccac tggatcgagg atttggcgct gtccggaacg cgtcggtttt gcttgagctg
       61 cccgccgtac gggtatccgg cggttgaacg aagaaaccac gaagggaaga ccgatgggta
      121 tcaccgacaa ggtgaccggt aaggccaagc aggttgcagg cgatgtttcg ggcgacagct
      181 cgctgcgccg caagggccgc aacgaagaga agaagggcga gaagaaggac gagctccacc
      241 gcgccgacga gcgcgcgaac gagaaggccg acgaggtcgc cgacctcgag cgcaagacct
      301 gatctcccgc cgagcgctgg ctgtgtgagt cgtcgctcgg ccaaggcatg gcccggagcc
      361 tcggttggct ccgggctttt gctgctcatt aacgagacaa acgagaaaga gcgagcgcaa
      421 cctggggcgt ggtcgagatt cgccgcccac tgcgccgctc agacccgctc cttcccagca
      481 cgggagtcgg ccctcaagac tccgggacga tctgtgcgat caggcgctac gggtaatgaa
      541 ggcaggagcg cgtcagttag cagccctttt gcgcgggctc agccgccacc ctcactgccc
      601 tcaccccgcg ttcgaaacac ggccgccgtg ggtagcgggg ctattgatgg gcatcacact
      661 ggagttggag ccggggccgg aggctgcgca ccaagcgcgc gatgaggttc gctatcacct
      721 ccgcgaccgg gtagaaaacg gcttgctaac cgacttgcag ctgatcgtca gcgagctcgt
      781 gacgaactcc gtgcggtacg gacggggcga gacaatcgag gttgagatcg ccgtggctga
      841 ggatggttcg atcatcggcg agatcgagga ccacggcagg ggcgaagttg cgatgcgaga
      901 gatcactgac cagggcggcg gctttggcct gcggatcatc gacgccctgg cgagccgctg
      961 gggcgtctgt gaaggcagca cccaggtgtg gttcgagctc agctgagccc ctggcccagc
     1021 cccgctctca gaaccggacg ggggtagcct ctgcatatgc ccggagagcg cgagcggcgg
     1081 ctagggaaga acgagaccct cttccggact gccaacgagc ggatggcagg ctgggaggaa
     1141 cgagaccgcc tcgaagcggt cgagctctac ttctgtgagt gcgccgaccc cacttgcatg
     1201 aagaaggcca ggctatgcgg ctccgattac gagcgggtgc gcagtgatcc gacccacttc
     1261 ttcgtcgtcc ccgggcacga ggtcgcggat gtggaaacgg tgctcgagtc gcacccggat
     1321 tgggtccttg tcgaaaaggc tgagtccgag gcccgcgacg tagccgagga aaccgacccc
     1381 cggcaggact aggccacacc atgtgagcgg ggtggcgcct agcttccgct gctcgcgcaa
     1441 cagaagcgcg gcctactccc gcagcccggc ggcttcgagg gccgggtccc gttcgttgta
     1501 aatctccagg tgggccatct tcccgtcgcg ggccgtgatg acaaaccaaa aggtccccgt
     1561 gatcggctgg tcgctcccca ccaagcgacc ctcttgcggg acttcgacta ccaccttgtc
     1621 cccggcatcc atgaactcgc tgggacggaa ctcgagttcc tcatacggct ccagccactg
     1681 gctcgcgaac tcgatgtacc cctccagccc gtggtaggtc tcggcatcga tctggccgac
     1741 ctgacggcag gtgacatcct catgaatgag gctcaggtgc gtctccaggt cgccctgctc
     1801 ccacgcctcg aagccgcgcc gcgcaagctc cacattctcc tgcgacatcg cctgcgcagt
     1861 atctcgcgcg cggagggaaa ggcaaagggg gccactgccg cttaccgcca ggaggacggt
     1921 agagatggat ggcagcggac cccccttgtc gtcgtatggc accaccgata tacccggacg
     1981 gagccggacc atgcctggga ggagggtcag aacccggacc cgtggatctc ggagaccgcc
     2041 cgggccgtaa acggctcgcg ccgcaaggaa catgcaattg agtaaccccg actgtctact
     2101 cccgcagccc ggcggcttcg agggcttcgg atcggtccag aacgcgccct actggctctg
     2161 ctcgttgatg ccgtagacgc ggccgtcagg gcctctgaag tggcagtagg cgtagttcat
     2221 ctcagggatg cgatgaatgt cgcacaagac ctcgaagcct gccgcttgga gttcggcgga
     2281 cgcggcttcg atgtcgtcga cacggaagcc gaagacgtgc cgtcgttcaa accgtagctc
     2341 ggaatgccct ccgagtccga ctcatagagg tcgaggatgg tgccgttcgg catggggaaa
     2401 gcgtccagcc ctcctcctcg accatcggcg tgagaccgaa aacctcgcca cagagcttct
     2461 tcatcgcggt gaactggtct ggctcgagag tgacggcatg ccaggtgatg ccctggacct
     2521 gcacgcttct accccctggg ttgggaaggt ggggtcggag agtgtatggg accaagctgt
     2581 cgaaaccgag acttattgag gtgggttgag gcatcccacg acgtccacgt tcttctgcga
     2641 catcgcctac tccgaaagcc cggcggcttc gaggtccggg ttcccgttca cgcgggagg
//
LOCUS       DASRJQ010000604         5040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_119635, whole genome shotgun sequence.
ACCESSION   DASRJQ010000604 DASRJQ010000000
VERSION     DASRJQ010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119635"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1568)
                     /locus_tag="VFB89_13710"
     CDS             complement(<1..1568)
                     /locus_tag="VFB89_13710"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZM28398.1"
                     /translation="MSESTAADLFETLQAVLQPQYRLQRELGRGGMGVVFQAVDLALD
                     RRVAVKVAHPELAAQPSVAERFLTEARMLARIRHPNIVTVHNAGSVGGHLYYVMDEIP
                     GESLRQRLNREGSLEPADVEQIMADIAAALDAASAAGFVHRDVKPENILLDAVSGRAL
                     LADFGIARASGEPGQVTRTGQGVAVGTPTYMSPEQAAGESVDTRSDVYSLGVVAYEAL
                     VGQPPFVGPRRVVVSKHIAERPMPIQRARSGVPAPLGAVIMRALEKHPADRWQGGEAM
                     RRALAERRAPGRLSRPRIAIAAIGTLAVVGVLGLTRLRSEAPPAGIDPRHSILVLPFQ
                     NLRADRPMDWMRDGAVSMLGLNLSQWSDLTVVDHERVHDLLAHHAVKPGDAIGLELAR
                     QLAREAGVWTVVLGEYSRSGDSLYLSARVFDVATGKRVEIGRIEGRPGTDVRPMFDEL
                     AARLLDLSGAPADIRTGLAQATTSSVEAFRSYLNGVEQLNRWNLPAAQRDFERATSLD
                     TTFSLAFYKLALARG"
     gene            1740..2516
                     /locus_tag="VFB89_13715"
     CDS             1740..2516
                     /locus_tag="VFB89_13715"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZM28399.1"
                     /translation="MSLAAAPLAQPSPTVAAVLLPGERSRVDAAGNGCFSVVHRDSIS
                     DALRVVRERPVDAVLVSVHRCAPEQVEALAHLVRDFPAIPMVALVSQHDAGATEMLLR
                     LGATGVRQVVDVTSPTGWNRLRQVVGQPATRAVARIQGPILEALAHAPPDARLFMEAL
                     IRLAPETPTVRRLADRLFVRPSTLMSRFVRSGLPSPKNYLAAIRLLHAALLFETSGLS
                     VADVAYRLEYSSPQSFGRHLRACSALPHRSFAPATLSRSL"
     gene            complement(2612..3157)
                     /gene="pyrE"
                     /locus_tag="VFB89_13720"
     CDS             complement(2612..3157)
                     /gene="pyrE"
                     /locus_tag="VFB89_13720"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HZM28400.1"
                     /translation="MTPLEQLLLAKSVRYGDFVLASGQRSSYYIDCRLTTMSAEGMVL
                     VGRAGLTAIREAGWTATAVGGLTLGADPVAYAIAAASFGTGAVLDAFTVRKETKGHGT
                     QRLIEGNFAPGVSVVIVEDVITSGTSALQAIQAVSNEGGRIAGVLAVVDREQGGRRVL
                     EAAGHQVRSLTTASAMGLGST"
     gene            complement(3154..4053)
                     /locus_tag="VFB89_13725"
     CDS             complement(3154..4053)
                     /locus_tag="VFB89_13725"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZM28401.1"
                     /translation="MPPENLVGRTVAGYRLVQHVGEGGTASVYKGEHPERGLAAVKIL
                     IPRLAKDPVAIKRFLREAEFGGRLNHPNIVRTYDYGEAEGLNYLALEWAEGEPLASFV
                     TRSGMLAPTLVATIVEQIGAALSAAHKAGIIHRDLKPANIMYDPATQRAKLLDFGIAR
                     DAEGSPEERLTRAGFFVGTLQYVAPETLSGELVGEQADVYSLATITYYSLTECLPFPG
                     RSPRELFQQLLSQDPIPLGQAKRGLRFSPAVETAVMNGLQRDLAKRSKSVDEFAQGFC
                     TAVRNEGGQKGGFLANLFRKGPR"
     gene            4114..>5040
                     /locus_tag="VFB89_13730"
     CDS             4114..>5040
                     /locus_tag="VFB89_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28402.1"
                     /translation="MPLLDARRVPPLTFARTPEEAQLYLHDDRRLSMRRLALVLSIAG
                     AVAACGGGGAGKGGSAGGGPAPAPDDMPAVTSTTGIYRGVGRLAAADPLPFVGNVAFA
                     QGVGDTTFAILGLSLENRSLNFQREPSGFTARYRVDITLRPVTGAPVQVGREETVRVA
                     TQPETGRTDESVLFQQHFKLLPGPYNVIVNVRDMNAGVESRAEGDYMVPKLAPGTSSE
                     PILAYQVQGRAKPADPLKLVINPRGVVSFGGDTLLAYIEGYAFPRRQTLPFEVRDGQD
                     SLVYRDSLRFQGGRPIESQVIRIAPDSLVLGEM"
BASE COUNT          831 a         1723 c         1585 g          901 t
ORIGIN      
        1 ccccgcgcca acgcgagctt gtagaaagca agactgaacg tggtgtcgag gctcgtggct
       61 cgttcaaagt cgcgctgcgc tgcgggaagg ttccaccggt tgagctgctc gacaccgttc
      121 aggtaggacc ggaaggcctc caccgacgag gtggtcgcct gcgccagtcc cgtgcggatg
      181 tccgccggtg cgccggacag atcgagcagc cgggccgcca actcgtcgaa catcggtcga
      241 acgtcggtcc ccggacggcc ctcgatccga ccgatctcga cccgctttcc tgtcgccaca
      301 tcgaataccc tcgccgagag gtagagcgag tcaccgctcc ggctgtactc ccccagcacc
      361 acggtccaga cgccggcttc gcgggcgagc tgtcgcgcca gctcgagccc gatagcatcg
      421 cccggcttca cggcgtggtg ggcgagcagg tcgtgcaccc gctcgtgatc caccaccgtg
      481 agatcgctcc attgagagag attgagtccg agcatgctga ccgccccatc ccgcatccaa
      541 tccattggcc ggtcggctcg gagattctga aacggaagca ccagaatcga gtggcgtggg
      601 tcgatcccgg ccggcggcgc ctccgaccga agtcgcgtaa ggccgaggac gccgaccact
      661 gccagcgtgc cgattgccgc gatggcgatt ctgggacgcg aaagcctgcc cggcgcccgc
      721 cgctccgcca gcgctcgcct catcgcttcc ccgccctgcc accgatcggc cggatgcttt
      781 tccaacgcac gcatgatgac tgccccgagg ggtgcgggca cgccggacct ggcacgctgg
      841 ataggcatcg gccgctccgc gatgtgcttc gacaccacga cgcggcgggg gcccacgaac
      901 ggcggctgtc cgaccagggc ctcgtaagcg acgacgccca gcgaatacac gtcgctccgc
      961 gtgtcgaccg actcgccggc ggcctgctcg gggctcatgt aggtcggcgt gccgacggca
     1021 accccctgcc ccgtccgggt cacttggccc ggttcacccg aggcgcgcgc gattccgaag
     1081 tccgccaaca acgcccggcc gctgaccgcg tcgagcagaa tgttttcggg cttcacgtct
     1141 cgatgcacga agccggcggc gctcgccgcg tccagtgccg cggcaatatc cgccatgatc
     1201 tgctctacat cagccggctc gagcgatcct tcccgattga gccgctgccg gaggctttct
     1261 ccgggaatct catccatgac gtagtacaaa tgtccgccga ccgatcccgc gttatgcacg
     1321 gtcacaatgt tggggtggcg gattcgcgcg agcatccgcg cttcggtgag aaagcgttct
     1381 gcgactgaag gctgagctgc cagctcgggg tgagcgacct tcacggccac tcggcggtcg
     1441 agcgcgaggt cgacggcctg gaacaccaca cccatgccgc cacggcccag ctcgcgctgg
     1501 aggcgatact gcggctgcaa gacggcctgc aaggtttcga acagatctgc tgcagtagac
     1561 tcgctcacgg cacctgcagg tccgacgtag gggtcgagag ccgggaggac gggagcaata
     1621 acgcgcgcaa taaattcgat gcggcgccgc cgcggaacgt tgcgttgcca ggtccgtcgg
     1681 cgacgttgac ccgcgattga gatgacccgg ctagcttggg cgcgactcga ggaggtcgca
     1741 tgtcactcgc cgccgccccg ctcgctcagc catcgcccac cgtcgccgcc gtgctgctgc
     1801 ccggtgagcg atcccgagtc gacgcggccg gcaacggctg cttctctgtc gtgcatcgcg
     1861 actcgatctc ggatgcgttg cgcgtcgtgc gggagcgacc ggtcgacgca gttctggtct
     1921 cggttcaccg ctgcgcaccc gaacaggtcg aggcgctggc ccacctggtg cgagactttc
     1981 ccgccattcc catggtcgcg ctggtctcgc agcacgatgc cggtgccacc gagatgctgc
     2041 tccggctggg tgccacaggc gtgcgccagg tagtcgacgt aacctcgccg accggctgga
     2101 accggctgcg tcaggtggtc ggtcagcccg ctaccagagc cgttgctcgc attcaagggc
     2161 cgatcctcga agcgctcgcc catgcgccgc cggatgctcg cctctttatg gaagcgctga
     2221 tccggttggc accggaaacg ccgacggtcc gccgcctggc ggaccggctc ttcgtccggc
     2281 cgagcacgtt gatgtcgcgt ttcgtgcggt ccggtttgcc ttcacccaag aactatctgg
     2341 ccgcgattcg gctgctgcat gcggcgctgc tgttcgagac gtcggggctt tcagttgccg
     2401 atgtcgccta tcggctggag tactcgtccc ctcagagctt tggtcgccac ctccgggcat
     2461 gctcggcatt accgcatcgg agtttcgcac ccgctaccct ttcccggtcg ctgtaggtgg
     2521 ctttgtcgag agactgctcc agccttacga ggcgatctgg tctacttttc acccgctgac
     2581 ccaagggagt tgggagggca acgcctagtc gtcaggttga ccccaacccc attgccgacg
     2641 cagtagttag cgacctaacc tggtgaccgg ccgcctccag cacccgtcgc ccgccctgct
     2701 cccggtctac caccgccaac acaccggcga tcctcccacc ttcgttgctg accgcttgga
     2761 tagcctgcag ggccgaggtg ccggacgtga tcacgtcctc cacgatcacg acggacactc
     2821 ctggcgcaaa gttcccctcg atcaggcgct gggttccgtg ccccttggtc tctttccgca
     2881 cggtgaacgc gtccagcact gcgccagtcc cgaagcttgc cgccgcaata gcgtatgcga
     2941 cgggatccgc tccaagggtc aacccgccaa ccgcggtggc ggtccaccca gcctctcgaa
     3001 tcgccgtaag tccggcccgc cccaccagga ccatcccctc agctgacatc gttgtgaggc
     3061 ggcagtcgat gtagtacgac gaccgctgac ccgaggccag cacaaagtca ccgtaccgaa
     3121 ccgattttgc cagcagtagt tgctcgagag gggtcaccga gggcctttgc ggaacaggtt
     3181 ggccagaaac ccacctttct gacctccctc gttccgcact gccgtgcaga atccctgcgc
     3241 aaattcgtcc accgacttac tgcgcttggc caggtcgcgc tggagcccgt tcatcacggc
     3301 ggtctcgacc gctggggaga accggagccc ccgctttgcc tggccgagcg ggattggatc
     3361 ctgtgagagt agttgctgaa acaactcacg tgggctccga cctggaaacg gcaggcactc
     3421 cgtcagcgag tagtacgtaa ttgtcgcgag gctgtacaca tccgcctgct cgccaaccag
     3481 ttcgccgctc agcgtttcgg gcgccacgta ttgcagggtt cccacgaaga atcccgccct
     3541 ggtcagccgc tcctcggggc ttccctcggc gtctcgtgca ataccgaaat cgagcagctt
     3601 agcacgctgc gtcgctggat cgtacatgat gttggcaggc ttgagatcgc gatggatgat
     3661 gcccgcttta tgcgccgccg agagcgccgc gccgatctgc tcgacgatcg tcgcgaccaa
     3721 cgtgggcgcg agcatccccg accgcgtgac gaagctcgcc aacggctcac cttcggccca
     3781 ttcgagggcg agatagttca gcccctcggc ttcgccgtag tcgtacgtcc gcacgatgtt
     3841 cgggtggttg agcctaccac cgaactcggc ctcgcgcaga aaccgcttga tggcgacagg
     3901 atctttggca aggcgaggga tgagaatctt gaccgcggcc aggccgcgct caggatgttc
     3961 ccccttgtac acactcgcgg tcccgccctc gccgacatgc tggacgaggc ggtaacccgc
     4021 caccgtgcga ccgacgaggt tctcaggggg catccgtgag ccaacctagc gggtcgccgg
     4081 atggccagca agctcactct cggagagtgt atcgtgccgc tgcttgacgc tcgtcgcgtg
     4141 ccgccgctaa ccttcgctcg cacgcctgaa gaggcgcagc tttacctcca cgacgaccgg
     4201 agactgtcga tgcggcgcct cgcgctggtc ctttcgatcg cgggcgctgt cgcagcgtgc
     4261 ggaggcggcg gagcggggaa gggaggctct gcgggcggcg ggccggcgcc agctcccgac
     4321 gacatgccgg ctgtcacgtc cacgaccggc atctatcggg gtgtcggtcg tctcgcggcg
     4381 gccgatcctc tgcccttcgt tggcaacgtg gcgttcgctc aaggtgtcgg cgacaccact
     4441 ttcgcaattc tcggtctttc gctggagaat cgctccctca acttccaacg cgagccgtcg
     4501 gggttcaccg ctcgttatcg ggtggatatc acgctccgtc cggtgaccgg ggcgcccgta
     4561 caggtgggtc gcgaggaaac ggtccgggtc gcgacgcaac cggagactgg tcgaaccgac
     4621 gagagtgtat tgtttcagca gcacttcaag ttgctgccgg gtccttacaa cgtcatcgtg
     4681 aacgtgcggg acatgaacgc aggggtcgag tcacgggccg agggcgacta tatggtcccg
     4741 aagctcgcgc ccggaacctc gagcgagccg attctcgcgt atcaggtgca gggcagggca
     4801 aagccggcgg atccgctcaa gctcgtcatc aacccccgag gcgtggtcag ctttggcggc
     4861 gataccctgc tggcatacat cgaaggctat gcctttcctc gccgacagac attgcccttc
     4921 gaggtgcgcg acggacagga cagcctggtg tatcgggact ccctgcggtt ccaggggggg
     4981 cggccaatcg aaagccaggt aatccgcatc gcacccgata gcctggtgtt gggcgaaatg
//
LOCUS       DASRJQ010000605         2059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2658227, whole genome shotgun sequence.
ACCESSION   DASRJQ010000605 DASRJQ010000000
VERSION     DASRJQ010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2658227"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VFB89_13735"
     CDS             <1..276
                     /locus_tag="VFB89_13735"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HZM28403.1"
                     /translation="GNNFFRSDRNRTANPAVDTIAAGGKVTWKWVNTGSVPHNVQSVG
                     MPSFMSGSIETGNGSTYELSFSAPGTYQYNCAIHGNLMTGVIVVTPQ"
     gene            498..1343
                     /locus_tag="VFB89_13740"
     CDS             498..1343
                     /locus_tag="VFB89_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28404.1"
                     /translation="MRLSIGIALLSAIAAAQPLAAQGECLPGSASHEAKTMALLSLPL
                     VFSRAGAPELLPGIRVGLEGAYVPHIDAATATPTFCRPGKGPEDVNRLPALPRPRLAL
                     PLPFGLAFEASWVPPIRVNGVRANLIGLSLDKTFGKLDGFVVGLRGYATLGSIRAPVT
                     CPDRALVDPLSECSGGTRSDDGFQPNILGADVAASWPMVGGRLLPYAGAGYNRMRPRF
                     QVNFTNRFNQLDDQRVSVDLDRMALFGGLTWRATPVLGLSSEIYAVPSDAVTARIVVR
                     RALVF"
     gene            1365..1982
                     /locus_tag="VFB89_13745"
     CDS             1365..1982
                     /locus_tag="VFB89_13745"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HZM28405.1"
                     /translation="MNKWMLITGMWLLSNGVQDAAESAPDWSAYTPRPVPSAAVAQGI
                     LSFDARATVGDFSGTTTTVRGEMTGGKDLSAVHGWVEAPVITLATGNERRDKDLNKSM
                     ESGRYPTMRFELAGVTPKAEQGDTVQVDLEGKFQIHGVERVVAIPAVVVLGTNAIRVQ
                     GSTALNLKDYRIGGLTKAWGMLRMYEGIVVHFDVTFAAVAGFSAS"
BASE COUNT          382 a          628 c          673 g          376 t
ORIGIN      
        1 ggaaacaatt tcttccgaag tgatcgaaac aggacggcga atcctgccgt cgacacgatc
       61 gccgccggtg gcaaggtgac ctggaagtgg gttaatacgg gttcggtgcc gcacaacgtg
      121 cagtccgtcg gtatgccgag cttcatgagc gggagtatcg agactgggaa tgggagcacg
      181 tatgagctga gcttcagcgc tccgggcacc taccagtaca actgtgcgat ccatggcaac
      241 ctgatgaccg gcgtcatcgt ggtcaccccg caatgagcgc gaatggccgc gtgaatcgcg
      301 agcgtagggt cgcagcctac agacggggaa cccgacagtt gatagtcggg gttgcgcccc
      361 gccgataacg gtagcgtacc gcgtttccta aggttgactc gactctagca gccacgggtg
      421 aacatcatgg ttagacgtcc gctcgcatac tgggccaccg agcctgccaa cgccgaagtt
      481 ctggtcgagg tgcgttgatg cgactctcaa tcgggatcgc gctcctgtcc gcgatcgctg
      541 cggcccagcc gctcgcagca caaggcgagt gcctcccagg cagtgcctcg catgaggcga
      601 agacgatggc gctgctctcg ctaccgctcg tcttcagccg cgcgggagct cccgagttgc
      661 tgcccggaat tcgcgttgga ttggagggtg cctacgtccc ccacatcgac gcagccaccg
      721 ccacaccaac gttctgccgg ccgggaaagg gccccgagga tgtcaatcga cttcccgcgt
      781 tgccccgccc acggctcgcg ctcccattgc cgttcggcct ggcattcgag gcaagctggg
      841 tgccgccgat ccgggtgaac ggcgtccgcg caaaccttat tgggctgtcg ctcgacaaga
      901 cgttcggcaa gctcgatggg ttcgtcgtgg gacttcgggg ttacgccacg ttgggctcga
      961 tccgggcccc agtcacgtgt ccggaccgtg ccctggttga cccactcagc gagtgctccg
     1021 gcggcacgcg ctcagatgac ggcttccagc cgaacatcct tggagctgat gttgcagcat
     1081 cgtggccgat ggtcggcggc cgactgcttc cctacgccgg cgccggatac aaccggatgc
     1141 gcccgcggtt ccaggtcaac tttaccaatc ggttcaacca gttggatgac cagcgcgtgt
     1201 cagtagatct cgaccgcatg gcgctcttcg gtgggcttac ctggcgagcg accccggtcc
     1261 tgggtctatc gagcgaaatc tacgcggtgc cgtctgacgc agttacggcg cggatcgtcg
     1321 tgcgccgtgc tctggttttc tgagtttcga gaaaggattc cgacatgaac aagtggatgc
     1381 tcatcacggg gatgtggctg ctcagcaatg gggtgcagga tgctgcggag tctgcgcccg
     1441 actggagcgc ctacacgcct cggccggtgc cgagcgccgc cgtggcgcaa gggatcctga
     1501 gctttgacgc ccgagcgaca gtcggcgact tcagcggtac caccacgacc gtccgcggtg
     1561 agatgacggg tggcaaagac ctgagcgccg tgcacggctg ggtcgaggcg ccagtcatca
     1621 cgctggccac cggcaacgag aggcgggaca aggatctgaa caagtccatg gagtcgggca
     1681 gatatccgac catgcgtttc gagctcgccg gtgtgacgcc gaaagcggag cagggtgaca
     1741 ccgtgcaagt ggacctcgag gggaagttcc agattcacgg cgtcgagcgc gtggtagcga
     1801 tcccggccgt ggtggtgttg ggaacgaatg caatccgagt ccagggctcg acggcgctga
     1861 acctgaagga ctatcggatc ggcgggctga ccaaagcgtg ggggatgcta cggatgtacg
     1921 aagggattgt cgtccacttt gacgtcacgt tcgcggcggt cgctggattc tccgcctctt
     1981 aaccctccct tcgtcgaaac gcgaaataac cgcccaccag catgagcgcg gccacgatcg
     2041 acaggacgat ggcaaatcc
//
LOCUS       DASRJQ010000606         3516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_360485, whole genome shotgun sequence.
ACCESSION   DASRJQ010000606 DASRJQ010000000
VERSION     DASRJQ010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360485"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VFB89_13750"
     CDS             <1..612
                     /locus_tag="VFB89_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28406.1"
                     /translation="LRDAAQSYHRPPDTPREQMWAEISARRLAARAAPRRPRLLVWAA
                     GIAAVLAIGIGIGRLSIRQRVAPAPTPAAPRVATTATPKEDITAYRVATAEHLGQAEA
                     FLTLFRASVRQGHQEQLASATARQLLATNRLLLDSPAAVDPKLRLLLQDLELVLAQIA
                     QLEPAHRNEDLRLITNGLEDGMLMRLRAAVPAGGATISRQGAL"
     gene            614..2086
                     /locus_tag="VFB89_13755"
     CDS             614..2086
                     /locus_tag="VFB89_13755"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZM28407.1"
                     /translation="MLWDFSWLTPLVAAVLTAIPCVTVTVGDDGLLSVPPASTYQADP
                     ADSVYRAARTALGRREYRQAAQLFASIETRFPKSQYAADARYWQAFSLYRLGSDESLR
                     QSLGALRIQRQRYPAAATKGDAAALERRVQGELARRGDADAAAAIAAEAQLVATPPAP
                     PAPPAPPAPPAPPAPPAPPAPPHPQKATALSTNGISRGCTEDDDDVKVAALNALQQID
                     PERAATVLKKVLARRDTQSVCLRRKAIFLLAQQGVEGAEPALLGAARQDPDPEVREQA
                     VFWLSQVGTDASVAALDSILRTTKDPVLMDKAAFALSQQGSDRAMQALRAFAERSDVS
                     EDGREKAIYWLGQSGTSENIAFLRGLYGRLRGEELKKKVMLSLAQTGTKENARWLAAI
                     ARNPQETAEMRKQALFWAEQCDLPTSEFATMYGSTTDRAMREQVIFVLSQRGDKAAAD
                     KLFEIAKSDPDPELRKKALFWLGQMDDPRVAEVLQEILEH"
     gene            2141..>3516
                     /locus_tag="VFB89_13760"
     CDS             2141..>3516
                     /locus_tag="VFB89_13760"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HZM28408.1"
                     /translation="MLPAILLLALGVSEPQLDSAVTTRPTPASAVPAARARSNITIDG
                     RLDEPAWSGTSPVTSLTQRVPQEGAPPTQRTEIRLLYDDDALYVGIRMYDTAPDSIKA
                     LLARRDRMVSADRFIVYLDSYHDRRSGFYFGINAGGTLYDGTLYNDEWSDSTWDGVWS
                     ARAVRDSLGWTAEFRIPYSQLRFERHDTYRWGVNFKREIARRKEEDYLVFRPTNGSGF
                     VSRFVDLIGLERVSPPPRLEVMPYVTAKAEFLDHAPGDPFHTGASANPGAGVDAKLGI
                     GSNLTMDATINPDFGQVEVDPAVVNLTDIETFYPEQRPFFIEGANIYNFGFGGANDFW
                     GFNWGGPEFLYTRRIGRAPQGRLPDAEFSNEPAGTHILGAAKLTGKAGSWSLGALNAL
                     TKREFADLATGDTRWRSEVEPPTWYGAYRLQKEMSGGRSGIGLIGTGVARWFDEDSLP
                     NQLAKNAFG"
BASE COUNT          642 a         1174 c         1113 g          587 t
ORIGIN      
        1 ctgcgcgacg cagcgcagtc ctatcatcgg ccccctgata caccgaggga gcagatgtgg
       61 gccgagatct cggcgcgccg gctcgcggcg agagccgcgc ctcgccggcc ccgcctcctg
      121 gtgtgggcgg caggcattgc cgcggttctc gcgatcggaa tcggcatcgg gcgcctcagc
      181 attcggcaga gggtcgcgcc ggctccgaca ccggcagctc cacgggtcgc gaccaccgcc
      241 acgccgaaag aggacatcac ggcctatcga gtcgcgacgg ccgagcacct tggccaggcc
      301 gaggcctttc taacgctgtt tcgggcgtcg gtgcggcagg gccaccagga gcaactagct
      361 tcggcaactg cgagacagct gctggcgacg aatcgcttgc tcctcgactc acctgccgca
      421 gtcgatccga agctcaggct gctgttgcaa gacctggagc tcgtcctggc gcagatcgcc
      481 cagctggagc ccgcacatcg aaacgaggat ctgaggttga tcaccaacgg actggaagac
      541 gggatgctga tgcggctgcg ggcggcggtt cccgctggcg gggccacgat ttctcgacaa
      601 ggagcgctct gagatgctct gggacttttc ctggctcacg ccgctggtcg cggcggttct
      661 aaccgcaatc ccctgtgtga cggtgaccgt gggggacgac gggttgctct cagtcccgcc
      721 cgcatcaacg tatcaagccg acccggccga ttcggtatat cgggcagccc gaacggcgtt
      781 gggccggcga gagtatcgcc aggccgcaca gctcttcgcc tcgatcgaga cccgtttccc
      841 caaatcgcag tacgcagcag atgcgcgcta ctggcaggcc ttctctctct accggctggg
      901 cagcgatgaa agcctgcgcc agtcgctggg agcccttcgg attcagcgtc aacgctatcc
      961 ggcggcggcc accaagggcg atgccgccgc actggagcgc cgggttcaag gagagctggc
     1021 ccgccgcggc gatgccgacg ccgcggcagc aattgcggct gaggctcaac tagtggccac
     1081 accgcctgct ccaccggcgc cgccggctcc acctgctccg ccggctccgc ccgcgccgcc
     1141 ggcccctccc gcgccgcctc acccgcagaa ggccacggcc ttgtcgacca acggcatttc
     1201 acgcggctgc accgaggacg acgacgacgt caaagtcgcc gcgctgaatg cgctccagca
     1261 gatcgatccc gagcgggccg cgacggtcct gaagaaagtt ctagcccggc gtgatacgca
     1321 gtccgtgtgt ttgcgacgca aggcaatctt ccttctcgcg caacaaggcg tggagggggc
     1381 cgagccggcc ctgctcggtg cggctcgtca ggacccggat ccggaagtgc gggagcaggc
     1441 agtcttctgg ctctcccagg tgggaaccga tgcatcggtg gcggctctcg attcgattct
     1501 ccggactacc aaggatccgg tgctgatgga caaggcggcg ttcgccctat ctcagcaggg
     1561 aagcgatcgg gccatgcagg ccctccgcgc gttcgcggag cgaagcgatg tatccgagga
     1621 cgggcgagag aaggcgatct actggctggg ccaaagcgga accagcgaga acatcgcctt
     1681 cctccggggc ctctacggcc gccttcgtgg tgaggagctc aagaagaagg tcatgctctc
     1741 cctggcccag accggcacca aggagaacgc acggtggctt gcggcaatcg cacggaatcc
     1801 gcaagagact gccgagatga gaaagcaggc cttgttctgg gccgagcagt gcgacctgcc
     1861 caccagcgag ttcgccacca tgtacggatc cactaccgat cgcgccatgc gcgagcaggt
     1921 gattttcgtg ctctcgcaac gaggcgataa ggccgctgcg gacaagctgt tcgagatcgc
     1981 caagagcgac cctgaccccg agcttcgcaa gaaggccctg ttctggttgg gtcagatgga
     2041 cgatccgcgg gtagccgagg tgcttcagga aattctggag cactagcgcg gcgaccgccg
     2101 gcaacgattt tgccgcgagc gccgtctaag ggcagcgtcc atgctgcctg cgatccttct
     2161 ccttgccctc ggcgtctcgg agccacagct cgactccgct gtgacgactc gtccgactcc
     2221 ggcctcggcc gttcccgcgg cccgggcgcg gagcaacatt actatcgacg gacgactcga
     2281 tgagccggcc tggagcggga ccagcccagt cacttcgctc acacagcgcg taccgcagga
     2341 aggcgccccg ccgacacagc gaaccgagat tcgtctgctg tatgacgacg atgcgctcta
     2401 tgtcggcatt cggatgtacg ataccgcccc ggattcgatc aaggcgctgc tggctcggcg
     2461 cgaccgcatg gtcagcgccg accggttcat cgtctatctg gattcgtatc acgatcgccg
     2521 ctcgggattc tacttcggca tcaacgccgg ggggacactc tacgacggaa ccctttacaa
     2581 cgacgaatgg agcgactcca cctgggacgg cgtctggagc gcgcgcgcgg tgcgcgattc
     2641 gctgggctgg accgctgagt tccggattcc gtactcccag cttcggttcg aacgccacga
     2701 cacctatcgc tggggcgtga acttcaagcg cgagatcgca cggcgaaaag aagaagacta
     2761 cctggtcttc cggcccacca acgggagcgg attcgtctcc cgattcgtcg atctgatagg
     2821 gctggaacgg gtttcaccac cgccccggct cgaagtaatg ccttacgtca cggccaaggc
     2881 ggaatttctc gatcacgccc ctggcgaccc tttccacacc ggcgcgagcg ctaaccccgg
     2941 tgccggagtc gacgccaagc tcggcattgg cagcaatctg acgatggatg cgaccatcaa
     3001 tcccgacttc ggccaggtcg aagtggaccc tgccgtcgtc aacctcaccg acatcgagac
     3061 cttctacccg gagcagcgcc cgtttttcat cgagggcgcc aacatctaca acttcggctt
     3121 cggtggcgcc aacgatttct gggggttcaa ctggggcggt cctgaattcc tctacactcg
     3181 gcggatcgga cgagcacccc aaggcagact ccccgacgcc gaattctcca atgagccggc
     3241 cggtactcat atcctcggcg cagcgaagct caccggaaag gccggcagct ggagtctcgg
     3301 cgcactcaac gcgctgacca agcgagaatt tgccgatctt gcgaccggtg acacccgttg
     3361 gcgctccgaa gtcgagccgc ccacgtggta cggagcctac cgcctgcaaa aggaaatgag
     3421 tggaggtcgc tcgggcatcg ggctgattgg caccggcgtg gcgcgatggt tcgacgagga
     3481 cagccttccg aatcagctcg ccaagaacgc gttcgg
//
LOCUS       DASRJQ010000607         5600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3894178, whole genome shotgun sequence.
ACCESSION   DASRJQ010000607 DASRJQ010000000
VERSION     DASRJQ010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3894178"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VFB89_13765"
     CDS             <1..333
                     /locus_tag="VFB89_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28409.1"
                     /translation="STLQATPQDPSRRMLMGLALAYMGRKSDAVREGERAAAMLPMEK
                     DAYTGPYLQHQLARIYVRVGQPEKALDILERLLKVPYYVSPGFLRIDPEWKPLHDNPR
                     FQRLVQSG"
     gene            326..3028
                     /locus_tag="VFB89_13770"
     CDS             326..3028
                     /locus_tag="VFB89_13770"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM28410.1"
                     /translation="MAEPARESLDRLQAALTGRYAIERELGRGGMATVLLARDLRHDR
                     PVALKVLHPELAATLGPDRFLREIRLAARLQHPHVLSVFDSGDADGQLWFTMPYVEGE
                     TLREQLRREGQLPIDDALRIARETADALDYAHQQGIVHRDIKPENILLTARHALVADF
                     GIARALGTAAEGFTREQRLTETGMSIGTPAYMSPEQASADREVGPRSDIYSLGCVLYE
                     MLAGEPPFTGPNAQAILLRKFTENPRPLRSLRDTVPPAVEEAVTKALARSPADRFGTA
                     GEFVKVLETAGVRTTSSAAAATSTVIGSAPATAARRPALLLRYPLVATLLVGIAIGLG
                     VLFAWRRGRATDEPASGTVKTVAVLPFENLGGAGDEYFSDGITDEVRGKLSSLTGLQV
                     TARSSSAEYKKTHKRPQEVGRELGVDYLLTGTVRWEKGQGGASRVRVSPELIQVSTGA
                     TKWQQPFDAALTDVFQVQADIAGRVAEALNVAIGANAREKLAERPTQNLAAYDAFLKG
                     EAAYAQGNNPPTLREAVGYFEQAVALDSTFAVAWAGLSQAQSLLVGTTAYSPGRADQS
                     RSAAERALALAPGRPEGYTGLGDYYRRVPNDPSRAIEQYNKGLRLAPNNADLLRGLSI
                     AEQSLGRWEQAIDHLRRGQSVDPRSGSVASILGQALLWTRRYPEALPAFERAIELRPG
                     NLQSLEGKAMVYLAKGDLPGARSVLASAPKDLDPTAFVAYIGTFWDLFWVLEPEQQNL
                     LLRVTPRPFDDDVGNWGLALAGTHYIHGDFAKARAYADSARAGLEQQLKVAPEDAQRH
                     ALLGVALAYMGNKADAIREAERALAIQPVSKDAVSGAYNQHQLARIYILVGEPDRAID
                     QLEALLKIPYYLSPGWLSIDPAFDPLRKNPRFQRLVEQGQKREQ"
     gene            complement(3076..3846)
                     /gene="mazG"
                     /locus_tag="VFB89_13775"
     CDS             complement(3076..3846)
                     /gene="mazG"
                     /locus_tag="VFB89_13775"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HZM28411.1"
                     /translation="MQDNSALGRAVAMVRDLRVRCAWDRVQTRETLRPYLVEEMLELD
                     QALAENDASAIRDELGDFLLHLAWQLVLGEERNEFTARDVAETMERKMKRRHPHLFDI
                     GPPDAWERLKRRERDDGVLGRLPAALPDLLRAYRLQERAASVGFDWPDARGPIEKVRE
                     ELTEVEKELARSDHRVSGGVANDALADEIGDLLFAVVNLARKAGVQPGPALDRANRKF
                     RDRFEAVEKLAEERGVDLHDAGLEVLDGMWEEVKLRER"
     gene            3880..4959
                     /gene="alr"
                     /locus_tag="VFB89_13780"
     CDS             3880..4959
                     /gene="alr"
                     /locus_tag="VFB89_13780"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HZM28412.1"
                     /translation="MPTTFSNETDRAWVDVDLSALVSNAKTITTISGGRLLPMVKANG
                     YGLGAIAVTRALEAVDPWGYGVATVEEGAELRHAGITRPILICTPLLPQWIDRCLELD
                     LRPSIGDLGALDAWMARGRRPFHLEIDTGMSRSGFRYDDPGLRGPIIERLRRAEGWEG
                     VFTHFHSPETDLATTELQWARFQEIIGILPRRPALVHAANSAAALTGNRYTADLIRPG
                     IFLYGGFAGTPLPAPVASLRGRVVGVRQIREGDTVSYGATWRADAPTRVATIAVGYAD
                     GFPRATGRSRGIELGGRVVPVVGRVAMDMTMVAVDDDQKVTLGEVATAWGGAVSLDQQ
                     ADWVGTNAYELLTALSPRLPRCYRA"
     gene            4959..>5600
                     /locus_tag="VFB89_13785"
     CDS             4959..>5600
                     /locus_tag="VFB89_13785"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF003766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HZM28413.1"
                     /translation="MRRAALIVLDGLGIGAAPDAECYGDVGSNTLGNLARQVGGLQLP
                     NLELLGLGSCGELSGVPAAKEPAGAHGVAEPISPGKDSTTGHWELCGLVLEQAFPTYP
                     QGFPGEIIDEFARRTGRGVLGNKPASGTAILDELGEAHCRSGDWILYTSADSVFQVAA
                     HEQTVPVTELYAACRVARAILQPPHGVSRVIARPFTGSPGAWTRTAGRKDFSLP"
BASE COUNT          968 a         1879 c         1762 g          991 t
ORIGIN      
        1 tcgactcttc aagccacacc gcaagaccct tcgcgccgca tgctcatggg ccttgcactc
       61 gcgtatatgg gccgtaagtc ggatgcggtt cgggaaggcg agcgtgctgc ggccatgctg
      121 ccaatggaaa aagacgccta caccggcccg tacctccagc atcagctggc gcgcatctac
      181 gtaagagtcg gccagcccga gaaggcgctc gacattctcg agcgactgct gaaggtcccc
      241 tactacgtat cgccagggtt cctccggatc gaccccgagt ggaagccgct ccacgacaac
      301 ccccgattcc agcgcctggt gcaaagtggc tgagcctgcg agagaatcac tcgaccggct
      361 tcaggccgcg ttgaccggcc ggtacgcgat cgagcgcgag ctgggtcgcg gcggcatggc
      421 caccgtctta cttgcccgcg atcttcggca cgatcggccg gtcgcgctga aagtccttca
      481 tcctgagctg gcagcgactc tcggtcccga ccgttttctc cgcgagattc gcctggccgc
      541 acgacttcag catccgcacg tgctgagcgt cttcgactca ggcgacgccg atggccagct
      601 ttggtttacg atgccatacg tcgagggcga gaccctgcgt gaacagctga ggcgtgaagg
      661 tcaactcccg atcgacgatg cgcttcgaat cgcgcgcgag actgccgatg cgctcgacta
      721 cgcccatcag cagggcatcg tccaccgcga catcaagcct gaaaacatcc tgctcacggc
      781 gcggcatgct ctggtagccg atttcgggat tgctcgtgcc cttggtaccg cggccgaggg
      841 attcacgcgg gagcagcggc tgacggaaac cgggatgagc atcggtaccc cggcgtacat
      901 gagtcccgaa caggccagcg cggaccggga ggtggggcct cgaagcgaca tctactcgct
      961 cggctgcgtg ctctatgaaa tgctggccgg cgagccgccg ttcacgggac cgaacgcgca
     1021 ggccatcctc ctgcgcaagt tcaccgagaa cccgcggccg ctccggtcgc tgcgcgatac
     1081 tgtgccgcca gccgtcgaag aggccgtgac caaggcgctc gcccgttcac cggcggatcg
     1141 gttcggcacc gcgggcgaat tcgtcaaagt gctggagacc gccggcgtca ggacgaccag
     1201 cagcgcggca gctgcgacgt ctacagtcat cggctcggcg ccggccaccg ctgcgcgccg
     1261 gcccgctttg ctgctccgct acccgctggt cgcgaccttg ctcgtcggca tcgcgatcgg
     1321 gctgggcgtt ttgttcgcct ggcgacgcgg ccgcgcgact gacgaacctg cgtccggcac
     1381 ggtcaagacc gttgcggtcc tgcccttcga gaatttgggt ggcgccggcg acgagtactt
     1441 cagcgacggc atcaccgacg aggtgcgcgg caagctctcg agtctgaccg ggctgcaggt
     1501 gaccgcccgc agcagctcag ccgagtacaa gaagactcac aagcgaccgc aggaggtcgg
     1561 gcgcgaattg ggggtggatt acctgctgac cggcacggtc agatgggaga aagggcaggg
     1621 tggggcgagt cgagtccggg tgagtcccga gctgatccag gtctcgaccg gcgccacgaa
     1681 gtggcagcag ccgttcgacg cggccctgac cgacgtcttt caggtacagg cggacatcgc
     1741 cggccgcgtg gcggaggccc tcaacgtggc gatcggcgcg aacgcccgcg agaagctggc
     1801 cgagcgaccg acccagaacc tcgcggcata cgacgcgttc ctcaaaggcg aagcggcata
     1861 tgcgcagggg aataacccgc caacgctccg cgaggccgtc ggctacttcg aacaggcggt
     1921 cgctctcgat tcaacgttcg ccgttgcctg ggcaggcctg tctcaggcgc agtcccttct
     1981 cgtcggcacc acggcgtact cgccgggacg ggcggaccag tcacgctcgg ctgcggagcg
     2041 agctctcgca ctcgcgccgg gccggcccga aggctacaca ggtctcggcg actactatcg
     2101 gagggtgccg aacgatccct cccgggcgat cgaacagtac aacaaggggc tccgcctcgc
     2161 gcccaacaac gcggatctgt tgcgaggctt gagcatagcc gagcagagtc ttggtcgttg
     2221 ggagcaggca atcgaccatc tgcgacgggg ccagagcgtt gatccgcggt ccggttcggt
     2281 cgcaagcatt ctgggccagg ctttgctctg gacccgacgc taccccgaag cgctcccagc
     2341 cttcgaacgc gcgatcgaac tccgtcccgg caatctgcag tcactagagg gcaaggcaat
     2401 ggtctacctg gcgaagggcg acctgcctgg tgcccgcagt gtgctagcat cggcacccaa
     2461 ggacctcgat cccaccgcat tcgtcgccta catcggcacc ttctgggatc tcttctgggt
     2521 gctggagcca gagcaacaga acctgctgct gcgagtcacg ccgaggccgt tcgatgacga
     2581 cgttggcaac tggggactcg cgctggccgg aacccattac atccatggcg acttcgccaa
     2641 agcccgggcc tacgccgact ccgcgcgcgc tggtttggag cagcagctca aggtcgcccc
     2701 ggaggacgcc cagcgacacg ccttactcgg tgttgccttg gcctacatgg gaaacaaggc
     2761 cgacgcaatt cgtgaagccg agcgtgccct cgctatccag ccggtttcca aagacgcggt
     2821 cagtggcgcc tataaccagc accagctcgc ccgcatctac attctcgttg gtgagcctga
     2881 cagagcgatc gatcagctcg aggcgctgct caagatcccg tactacctgt cgcctggttg
     2941 gctgagcatc gacccggcat tcgatcccct gcgaaagaat ccgcggtttc agcggttggt
     3001 agaacaggga caaaagcgtg aacagtgaac ggaagtgccc ttcgccttcg gagtcccccg
     3061 ttcaccgttt accgttcacc gttcacgcag tttgacttcc tcccacatcc cgtccaacac
     3121 ctccagacca gcgtcgtgca ggtctacgcc tcgttcctct gctagcttct ccacggcctc
     3181 gaaccggtcc cgaaacttcc gattggctcg atcgagcgct ggcccgggct gcacgccagc
     3241 tttccgagcc agattgacca cggcaaagag cagatcacct atctcgtcgg ccagcgcatc
     3301 attcgcaacg ccacccgaaa ctcgatggtc ggagcgcgca agctccttct ccacttccgt
     3361 cagctcctct cgcaccttct cgatcggtcc ccgcgcatcg ggccagtcga aaccaaccga
     3421 cgcagctcgc tcctgcagcc gataagcgcg aagcagatcc ggtaaggcag ccggaagccg
     3481 accgagcacg ccatcgtccc gttcgcgacg cttcaatcgc tcccacgcat ccggtggtcc
     3541 gatgtcgaag agatgcggat gacgccgctt catctttcgc tccatcgtct cggccacatc
     3601 gcgcgccgtg aactcgttcc gttcctctcc cagaacgagc tgccacgcga gatggagcag
     3661 aaaatctccc agctcgtccc tgatggctga cgcgtcgttc tcggccagag cctgatccag
     3721 ctcgagcatt tcttcgacca gatagggacg aagagtttcg cgggtctgca ctcgatccca
     3781 ggcgcatctg acgcggaggt cgcgtaccat tgccacagca cgtccaagcg ccgagttgtc
     3841 ttgcatttag gcctcgcgac gaattaggat tcgccctccg tgcctacgac tttttccaac
     3901 gaaaccgacc gggcctgggt cgatgtggat ctgagcgccc tggtgagcaa cgccaaaaca
     3961 attacaacga tctctggcgg gcgtctgctt ccgatggtca aagctaacgg ctatgggttg
     4021 ggggcgatcg cggtgacgcg cgcgctcgag gcggtcgatc catggggtta tggcgttgcg
     4081 accgtggagg aaggcgccga gctgcggcat gccggaatca ctcgtccgat tctgatatgc
     4141 accccgctgt tgccgcaatg gatcgatcgc tgcctcgaat tggacttgcg tccgtccatc
     4201 ggagatctcg gcgccctcga tgcctggatg gcccgcggcc ggcggccttt ccacctcgaa
     4261 atcgacaccg gcatgagccg ctccggtttt cgctacgacg atccggggtt gcgggggcca
     4321 atcatcgagc gtctgcgaag ggccgaaggt tgggagggtg tgttcaccca ttttcactcg
     4381 ccggagacgg acctggcgac aaccgagctg cagtgggccc ggttccagga aatcattggc
     4441 atcctgccgc gccgccccgc gctcgtccat gctgctaaca gcgcggctgc gttgaccggc
     4501 aatcgctaca ccgccgatct aatccgtcct ggaatttttc tctatggcgg gttcgccggg
     4561 acgccgctgc ccgccccggt ggcctcgctc cgcggccggg ttgtcggtgt ccgtcagatt
     4621 cgcgaaggcg acacggtgag ctacggtgca acctggcggg ctgatgcgcc cacccgggtt
     4681 gccaccattg cggttggcta cgccgacgga tttccccgtg ccaccggtcg ctcacgtggc
     4741 attgaattgg gagggcgcgt agtgccggtc gtgggcaggg tcgccatgga catgaccatg
     4801 gtcgcagtgg acgatgatca gaaggtcacg ctcggtgagg tggcgaccgc gtggggtggc
     4861 gccgtctcac tcgatcagca ggcggactgg gtcggcacca acgcctatga gctgctcact
     4921 gccctgagcc cgcgccttcc gcgctgctac cgagcttgat gcggcgcgca gcgctcatcg
     4981 tgctcgacgg gttgggcatc ggcgccgctc cggatgccga gtgctacggc gacgtcggca
     5041 gcaacacgct cggcaacctc gcccgccagg ttggggggct ccagctgccg aatctcgagc
     5101 tgttgggatt aggctcctgt ggtgagctga gcggcgtgcc ggccgccaag gagccggcgg
     5161 gtgcccacgg cgtcgccgag ccgatcagtc cggggaagga cagcaccacc ggtcactggg
     5221 agttgtgcgg cctggtgctc gagcaggctt tcccgaccta cccgcagggc tttcctggcg
     5281 agatcatcga cgagttcgcc cgccgtacgg gtcggggcgt cctgggcaac aagcctgcct
     5341 cgggcactgc tattctcgat gagctcggtg aagcccactg ccgcagcggc gactggattc
     5401 tctacacctc ggcggacagc gtcttccagg tggcggctca cgagcaaacc gtcccggtca
     5461 ccgagctcta cgccgcctgc cgggtcgctc gcgcgatcct gcaacccccc catggggtct
     5521 cccgggtcat cgcgcgtcca ttcaccggat cgccgggggc ctggacccgc actgcgggcc
     5581 gcaaggactt cagtcttccg
//
LOCUS       DASRJQ010000608         2228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3654635, whole genome shotgun sequence.
ACCESSION   DASRJQ010000608 DASRJQ010000000
VERSION     DASRJQ010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3654635"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VFB89_13790"
     CDS             <1..185
                     /locus_tag="VFB89_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28414.1"
                     /translation="DVRDTVDGRVRVALMVSGEADLRPHIFELAKAQGWTLFELHQEA
                     GSLEDLFHQLTTGEAA"
     gene            182..>2228
                     /locus_tag="VFB89_13795"
     CDS             182..>2228
                     /locus_tag="VFB89_13795"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HZM28415.1"
                     /translation="MTRIWTVARRELRALFDHPTGYVLLVVFLAVNGFLFFRQAYLQG
                     SATLRPMLDFLPWLLLFFVPAVTMRALAEDTRGGQLEVLLSQPITEAELLLGKYLGSV
                     LFLWIALAGTLPIPVGLAAGANLPWGAIAAQYVGAALLTAGLAGVGVWASSLTRSQMT
                     AFIAGVAVMFVLVLVGLDPLLVGLPASLGAVVARLGVLSHFESIGRGVIDLRDAIYFV
                     SLAGIFLALAFGSLMARKLSPGGSAAGRLRLGVGLLVALLVVTNLVGNHIGGRLDLTP
                     GRSYTLSRPTKQLVANLPDLVTIKVFASKELPTEVSLIKRDLDDLLRDIRTAGRGKVR
                     IIERDPADDPTAKQDAQDLGITPVQFNVLGQTELQVKQGYLGLAVQYGGGTEAIPLVQ
                     RTDNLEYRLASSIRTLTRSKKPVLGLMVTGAPPGLQISELQEQLAKSYDVRTINLADS
                     TQPGADVSVLALLGSPDSLTPAQGERFQRFFDRGGSALVLASGMQLSPQMPAAGPRAV
                     AWNRYLKPFGLEIRSNMAYDLMANEIVPLPSDIGQVLQSYPLFIRARSTAQSPINQEV
                     NEVTLTWTSTVDTTGAKGRVTPLLVSSRGSGTLTSEVSIDPTQSFPQNNLAPRVLGAA
                     AVGGGTAGAPKGRVVVVGSTDFINDRFVSRAPDNLSLALNAVDWLAQDDALIAIRSKD
                     "
BASE COUNT          353 a          722 c          713 g          440 t
ORIGIN      
        1 tagatgtccg cgataccgtc gacggccgcg tgcgcgtggc gttgatggtg agcggagaag
       61 ccgatctgcg tccccacatt ttcgagctgg ccaaggccca gggttggacc ctgttcgagc
      121 tgcaccagga ggcaggaagc ctggaggatc tgttccacca gctcaccaca ggggaggcgg
      181 catgacgcgg atctggaccg tcgcccggcg ggagctacga gcgctcttcg atcacccgac
      241 gggctatgtc ctgctggtcg tgtttctggc cgtcaacgga tttctgttct tccgccaggc
      301 ctacctgcag gggagcgcta cgctccgccc gatgctcgac tttctcccct ggctgcttct
      361 cttcttcgtc cccgcagtga ccatgcgagc gctggcggag gatactcggg gcgggcagct
      421 cgaggtcctg ctctcacagc cgatcaccga ggcagagttg ctgctgggta agtatctcgg
      481 cagcgttctg tttctctgga tcgctctggc gggcacgctg ccgattccag tcggcctcgc
      541 cgccggagca aaccttccgt ggggggccat tgccgcgcag tacgtcgggg cggctctgct
      601 cacggcgggc ctcgcgggtg tcggggtctg ggcctcgagc ctcacccgca gccagatgac
      661 cgccttcatc gcaggcgtcg cggtgatgtt tgttctggtg ctggtggggc tcgatccgct
      721 cctggtcggt cttccggcga gccttggcgc agtagtggct cggttgggcg tgctctctca
      781 tttcgagagc atcgggcgcg gcgtcatcga cctccgcgac gcgatctact tcgtctccct
      841 ggccggcatc ttcctcgccc tcgcgtttgg ctctttgatg gcgcgaaagc tctcccccgg
      901 cggcagcgct gcaggtcggc tgcgactggg cgttgggctc ctggtcgcgc ttctcgttgt
      961 gaccaatctg gtgggcaacc atatcggagg ccggctggat ttgactcctg gccgttccta
     1021 cacgctctcc cggcctacca aacaattggt cgccaatctc cccgacctgg tcacgatcaa
     1081 ggtgtttgct tcgaaggagc ttccaaccga ggtctcgttg atcaagcgcg acctcgacga
     1141 cctccttcgc gacatccgca cggccggccg cggaaaggtc cgcatcatcg agcgcgaccc
     1201 cgccgacgac cccactgcca aacaggatgc ccaggatctc ggcattacgc cggtccagtt
     1261 caacgtactt ggccaaactg agcttcaggt caagcagggc tacctgggct tggcagtcca
     1321 gtacggcggc ggcacggaag cgattccgct ggtccagcga accgacaacc tcgagtaccg
     1381 tctagcttcg tcgatccgaa cgcttacccg gtccaagaag ccagtgctcg ggttgatggt
     1441 gacgggggcg ccgccgggac tgcagatctc tgagctgcag gaacagctcg ccaagtcgta
     1501 cgatgtccga accatcaatc tggccgactc cacccagccg ggggcggacg tgtcggtgct
     1561 tgcgctgctc gggtcacccg actcgttgac tccggctcag ggagaacgct ttcagcgttt
     1621 ctttgatcgg ggcggaagcg cgctcgtcct ggccagcggg atgcagctct ctccgcagat
     1681 gccagccgcg ggcccgcggg cggtggcctg gaaccgttac ctcaagccat ttggcctcga
     1741 gatccgctcc aacatggcct atgacctgat ggccaatgag atcgtcccgc ttcccagcga
     1801 cataggtcag gtgctgcagt cgtacccgct gttcatccgg gcgcgcagca cggcgcagtc
     1861 gcccatcaac caggaagtga acgaggtcac cctcacctgg accagcacgg tggatacgac
     1921 gggggccaag ggtcgggtaa caccactgct cgtctcgagc cgaggctcgg gcacgctaac
     1981 gagtgaggtg tcgatcgatc cgacccagtc atttccgcag aacaacctcg cacctcgcgt
     2041 gctcggggca gctgcggtcg gcggtggaac cgcgggggca ccgaagggac gggtcgtggt
     2101 ggtgggcagc accgacttca tcaatgaccg tttcgtgagc cgcgctccgg acaatctgtc
     2161 gttggctctc aatgcggtgg actggctggc gcaggatgac gccctgattg cgattcgatc
     2221 caaggatc
//
LOCUS       DASRJQ010000609         9930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1618636, whole genome shotgun sequence.
ACCESSION   DASRJQ010000609 DASRJQ010000000
VERSION     DASRJQ010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 9930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9930
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1618636"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..1477)
                     /locus_tag="VFB89_13800"
     CDS             complement(191..1477)
                     /locus_tag="VFB89_13800"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HZM28416.1"
                     /translation="MITSRRVAIVAGVRTPFAKSGTVFRDVPAVQLARYAAKELLYRT
                     EIDGREIHQVIVGQVIASVLTPNVAREVSLLPQLPPGIPAYSLNRACASAAQAITNAA
                     DQIELGNADVVLAGGVESLSDIPILHSRRFSRILLEASKAKTLGGRLAAFGRVRPRDL
                     IPVTPAIAEPSTGESMGQSAEKMAKENGISREAQDRIALMSHLRAAAGTADGRLTSEI
                     VPWFGGPEMDDVATTDNGIRPDASLEALASLKPVFDRRYGTVTAGNSSPLTDGAAVVL
                     LMAEGKARSLGYEPLAFIRSYAVSAVDPGWQLLMGPALAVPKALERAGIGWNDLGLLE
                     IHEAFAAQVLSNVQAWCSQAWAERLGLSAPLGEVDWEKTNVMGGSIAIGHPFGATGAR
                     LVTTLANEMRRRDVEFGLVSICAQGGMGYAVVLDRS"
     gene            complement(1474..2022)
                     /locus_tag="VFB89_13805"
     CDS             complement(1474..2022)
                     /locus_tag="VFB89_13805"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZM28417.1"
                     /translation="MALSPVTRVPRPSTWFFWQAHFIRQGGRVLDLACGEGRHSLAAA
                     ALGANVLGVDRDAEQLAAARDAAGAKGLKAEFREVDLEAPWPKFGEFDVLLLFNYLDR
                     RRMADVLACIAPGGVLIMETFLDAQRELGWGPQNGEHLLQPGELGQLVRPLEVVHGRE
                     VLEPVDAERWRAVASVVAQRIQ"
     gene            2059..3762
                     /locus_tag="VFB89_13810"
     CDS             2059..3762
                     /locus_tag="VFB89_13810"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HZM28418.1"
                     /translation="MSVRPVDVLVIGGGITGAGIARDAAMRGLRTVLVEQADLGSGTS
                     SRSSRLIHGGLRYLEQGHLHLVWEANRERRVLLGIAPHLVRPLPFVFPLHRGDRIPLW
                     RLAAGMWAYDLLALFRNVAPHRMLGKRALLEREPMVRERGLIGGARYFDAQCDDARLV
                     LATARSAARHGANIATYTAVDGFEWAGDRVIGARVVDRLNGSRGTIRASALVNATGPW
                     VDRLRAMEEPGCRPLVRTTKGVHVIVPRDRLGHHEAIAFLSPIDGRVMFALPWGDLSY
                     LGTTDTDAPEPPESAGATADDIVYLLRSVNARFPNARLGLDDVRGSWAGLRPLLAPEH
                     DGKPSSRSREHAIVTGAGGIVTVAGGKLTTHRVMAAQAVDRVLAELTKRGISVRSGEA
                     RTDEEPLPGGETAALDPFRERALEIGLSAETADHLVRHYGTESAGIVNFAAIHRAYQQ
                     RLHPHHPAIEAEVLHMARRELACRVADVLMRRIHLFFETRDRGAAAAQRVAELLGREL
                     DWDAERLKGEIESYRKLASFPGETSYTSTESTWGSDSSTASTRPKESGEIGRPDSGNR
                     M"
     gene            4322..8077
                     /locus_tag="VFB89_13815"
     CDS             4322..8077
                     /locus_tag="VFB89_13815"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF008907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HZM28419.1"
                     /translation="MFETANAGFAQAMYEEFLRDPAAVGPEWRRLFESGVVGEKPNGA
                     DGAHRRTGTDLAAQVAFEVGAVEPARRTGDGEATGSAAPPEPISSPAPGREVARLPAG
                     ASPIKGPAARLVTNMTESLSVPTATTFRTLAVATLESRRKELNARLQTAGRKEKLSFT
                     HLIAFALIQAAKRHPVMGHTLLVQDGAVYRVQPEGIGLGIAVDVQRKDGSRGLVVPVI
                     KRADTMDFGEFHAAYEALVEKARANQLMPDDFSGSTIQLTNPGGLGTVASVPRLMAGQ
                     GSIIAVGAIGYPAEFSSVPDDKLRELGVSKVMTASSTYDHRVIQGAESGAFLATLDHL
                     LQGEDHFYETVAESLQLSAISYQLAPAATQLPKAPDTVAPEMLYHVAAAMALVKAFRT
                     HGHLAAHTDPLGTEPLGDPALDLGPLGLTPEIMAAIPSKVLRVYVPGRTLAESYPYLQ
                     QTYCGTMAFQVEHISSHQERVWLREKIESGAFRTPPSADEKKRLLARLTKVEALERFL
                     HKAYLGHKRFSIEGVDLLVPMLDLTLELAASTGAREIVIGMAHRGRLNVLAHIIGRPY
                     ETIFAEFEGGRFVEGNRLTPEGGTGDVKYHHGAEGAFPLRNGKSIAVTLSPNPSHLEF
                     VSPVVDGRARATQTLRKGRSAHHDPTIALPVLIHGDAAFAAQGVVAETLNLSALPGYG
                     VGGTLHIITNNQIGFTTDTQDARSTRYASDLAKGFDAPIIHVNSDDAEACLAAIRLAM
                     AYREKFRRDVLIDLVGYRRHGHNESDEPTYTQPVMYERIKQLPPVRSRYAEQLAAEGV
                     VSVEEATQQAESAYQRLVDAQQRFKASLGKGPVPEPPKPTGPGHEVATAVAPETLTAL
                     NEQLLTWPQNFAVNPKLKKQLERRRVAVGPEGGIDWAHAEALALASLVVEGVPVRLTG
                     QDTERGTFSQRHLVLHDVKTGQRFAPIQRLPGALAPLELHNSPLSELASLGFEYGYSA
                     EAAEALVLWEAQFGDFINGGQVIVDQFLSSGLSKWGLTTRLTLLLPHGYEGQGPEHSS
                     ARLERFLQLCAEGNIRVANPTSPAQYFHLLRRQARRTRQRPLIVMTPKSLLRHPQASS
                     RLEDLAEGTWRPVFDDPWATDERAAGVTRLVLCTGKLYYELLAEAEKMTAERPALIRL
                     EQLYSFPWSESRQVLARYPNVQQLIWAQEEPRNMGAWNYLDPKLRELLPAGAELRYVG
                     RPERASPAEGYPAAHMAEQSRIVADALGVKQPRLSRETVAPGGEGQG"
     gene            8605..9216
                     /locus_tag="VFB89_13820"
     CDS             8605..9216
                     /locus_tag="VFB89_13820"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HZM28420.1"
                     /translation="MSLLSSRITKPGFTITDTAGTRRLAVGVRAALAAWAVCLPLALS
                     ACRTAEAGSDVAAKRMIGNVLDRYTKAYRDNDPDAISKLYAQEAVLMPPGHELVEGRD
                     SVRAFWGRGMEPGFRMTTVRVEVSGSTAYVVGRYYVPPDDDDDAETGKYLIALRKEPD
                     GNWRITADIWNDDESDEEPDSTSDTTRQSVALRASARLRSGLP"
     gene            complement(9197..9646)
                     /locus_tag="VFB89_13825"
     CDS             complement(9197..9646)
                     /locus_tag="VFB89_13825"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HZM28421.1"
                     /translation="MGEVSFLRLVHVVGAVLLLGNVTITGFWAAFLFRARRVVPFRVV
                     ARAILWTDLVFTVGGGMMLVISGILLARARGYSLTATPWLLQGVIALIVSTGLWLAVL
                     LPDQWRLERAAPGDDATLRALFLRWSIVGWTATALLFYALWVMVART"
     gene            complement(9639..>9930)
                     /locus_tag="VFB89_13830"
     CDS             complement(9639..>9930)
                     /locus_tag="VFB89_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28422.1"
                     /translation="VAFRYVHLSEQLPEAFARIGRGWLLDPSHYADRRALMPLFEADL
                     LSVIDALDADLRTERVSMFRTVVAGRLSINKRMDRVRHAVGLLRGRFEPRNG"
BASE COUNT         1856 a         3205 c         3255 g         1614 t
ORIGIN      
        1 tgtcggcatt cagcgtattg accggctgat ccatgcgatc gagcgtgacg gtcgcgatgc
       61 cgttgtccgt tttggtcgag aagacaggca tttacgggac ctgcgtaaac ggtgaacggt
      121 gaacgggctt gctccgtggg cgggcgcccc ccgtttaccg tttacggttc accgttcacg
      181 ctgttaaggg tcacgatctg tccaacacga cggcataccc cattcccccc tgcgcacaga
      241 ttgacaccag cccgaactcg acgtctcggc gccgcatctc gtttgccagc gtcgtgacga
      301 gtcgagcgcc cgtggcaccg aacggatgcc cgatggcgat tgagccgccc attacgttgg
      361 tcttctccca gtcgacctct cccagggggg cggacaaccc taggcgctcc gcccaggcct
      421 gcgagcacca ggcctgaacg ttggagagca cctgggccgc gaaggcctca tggatttcga
      481 gcaggcccag gtcgttccag ccaatccccg cccgctcgag cgctttggga acggcaaggg
      541 ccggccccat gagcagctgc caacccggat caaccgcact cacggcatag ctccgaatga
      601 acgcaagcgg ctcgtagccg agagaccgcg cctttccctc ggccatgagc agaaccactg
      661 cggctccatc ggtgagcggc gaggagttgc cggccgtgac cgtaccgtac cgccgatcga
      721 agaccggctt caggctcgcc agcgcctcga gcgaggcgtc gggccgaatc ccgttgtcgg
      781 tggttgccac atcgtccatc tcggggccac cgaaccaggg aacgatttcc gaggtgagcc
      841 tgccatcggc ggttcccgca gccgcgcgca gatggctcat gagcgcaatc cgatcttgcg
      901 cttcgcgact gatcccgttc tccttggcca tcttctcggc cgactggccc atgctttcac
      961 cggtggaagg ctccgcaatg gcgggcgtaa ccggaatgag atcgcgcggc cggacccggc
     1021 cgaatgcagc caggcggcca cccagcgtct tcgctttaga ggcttcgagg agaatccggc
     1081 tgaagcgccg ggagtggaga atgggaatgt ccgaaagcga ctcgacgcca cccgcgagga
     1141 cgacgtcggc attccccagt tcgatctggt cggcggcgtt ggtgattgcc tgagccgccg
     1201 aagcacaagc ccggttgagc gaataggccg gaattcccgg cggcagctga gggaggaggc
     1261 tcacctcccg cgcgacgttg ggcgtgagca ccgacgcgat gacctgcccc acgatgactt
     1321 gatggatttc ccggccgtcg atctcggtgc ggtagagcaa ctccttggcg gcgtagcgag
     1381 ccagctgaac agcgggcaca tcccgaaaaa cggtgccgct tttggcaaag ggagtccgca
     1441 cgccggcgac gatggcgacg cggcggctcg ttatcattgt atccgttgcg ccacgacgct
     1501 cgcgaccgcc cgccagcgct cggcgtcaac cggctccaga acctcccgtc cgtggacgac
     1561 ctcgagcggc cttaccagtt gcccgagctc gcccggttga agcagatgtt cgccgttctg
     1621 cggcccccag cccaactcgc gctgggcgtc gaggaaggtt tccatgatca gtacgccgcc
     1681 cggcgcaatg cacgcgagga cgtcggccat ccgccggcgg tcgagatagt tgaacagcag
     1741 tagcacgtcg aactcgccga atttgggcca cggcgcctcg aggtccacct cccgaaactc
     1801 cgctttgagt cccttcgccc ccgccgcgtc gcgggcggcg gccagctgct cggcatcccg
     1861 atcgacgccc aggacgttgg cccccaacgc cgcggctgcc aggctgtggc gaccctcgcc
     1921 gcacgccaga tccagcacgc gcccgccctg gcggatgaaa tgagcctgcc aaaagaacca
     1981 ggtgctggga cgcgggacac gggtgacggg gctcagggcc acgcaagcca ctccttacga
     2041 cgaccggcgc tgggcgcatt gagcgtcagg ccggtggatg tgttggtcat cggcggaggc
     2101 atcaccgggg caggaattgc ccgggacgcc gcgatgcgag gactgcggac ggtgttggtc
     2161 gagcaagcgg atctcggctc gggtaccagc tcgcgctcga gccgcctcat ccatggaggg
     2221 ctccgctatc tcgagcaagg acacctgcac ctcgtttggg aagcgaaccg ggagcgccgc
     2281 gtcctgctcg ggatcgcacc acacctcgtg cgcccgctcc ctttcgtgtt cccattgcat
     2341 cgcggggacc ggattccact ctggcgcctc gcagccggga tgtgggccta cgacctgctg
     2401 gcgctgttcc gcaatgtggc cccccaccgc atgctcggca agcgtgccct gctggagcgc
     2461 gaaccgatgg ttcgggagcg cggattgatc ggcggcgcac ggtatttcga cgcccaatgc
     2521 gacgatgccc ggctggtcct ggcgactgcc cggtcggcgg cacggcacgg agcgaacatc
     2581 gcgacctaca ccgcagtgga cgggttcgaa tgggccggcg accgggtgat cggtgcgcgg
     2641 gtggtcgatc ggttgaacgg ctcgcgcggt accattcggg cctcggcgct ggtgaacgct
     2701 accggaccct gggtcgatcg gctgcgggcg atggaagagc ccggctgccg cccgctggtt
     2761 cgcacgacca agggggtcca tgtcatcgtt ccacgcgacc ggctgggaca tcacgaggcc
     2821 attgcgtttc tgagtccgat cgatgggcgg gtcatgttcg ccctgccctg gggcgatctc
     2881 tcgtacctcg gaaccaccga caccgatgcg ccggagccgc ccgaaagcgc cggcgcgact
     2941 gccgacgata tcgtgtacct gctccgctcc gtcaacgcgc ggtttcccaa cgcccgtctc
     3001 ggcctggacg atgtacgagg tagctgggcg ggattgcgcc cgctcttggc cccggagcac
     3061 gacgggaagc catcgagccg atcccgtgag catgccatcg taaccggtgc cgggggcatc
     3121 gtgaccgtcg caggaggaaa gctcacgacc catagggtaa tggctgcgca ggccgttgat
     3181 cgcgtgctgg cggagcttac caagcggggc atctccgtac gttccggcga ggcccggacc
     3241 gacgaagagc cgctcccggg cggcgagacc gccgcgctgg acccatttcg tgagcgagcg
     3301 ctcgagatcg ggctgtcggc cgaaacggcc gaccacctcg ttcggcacta cggaactgaa
     3361 tcggcgggga tcgtgaactt cgccgcgatt caccgcgcct atcagcagcg gcttcatcct
     3421 catcacccag ctatcgaggc agaggtgctt cacatggctc gtcgggagct cgcctgccgc
     3481 gtggcggatg tactgatgcg gcgcattcat ctgttcttcg aaactcggga tcgcggcgcc
     3541 gcagcggcgc agcgagttgc cgagctcctt ggccgggagc ttgactggga tgcggaaaga
     3601 ctgaaaggcg agatcgaaag ctatcggaag ctggcgtcat ttcccggtga aacgtcgtac
     3661 acgtccaccg agtcgacctg gggcagtgac tcgagcacag cgagcacgcg acccaaggaa
     3721 tccggcgaga tcggacgacc ggattccggt aaccggatgt agtacgcgcc ggccggctcc
     3781 gacccgccca cgacggttcc ccggaccgct gaaaccgcag ctcgccgctg ccggggcgac
     3841 gcgtcgggcc tgaagcttac gaccgcgata ttacggagca ccagactgcg gcgcccctcc
     3901 gggtcgctgg tcaaataggt gtcatcgtgg aaccaccgcg gggtgaagtc ggtcgaagcc
     3961 tgtctgccag aagccggcgg cagggtatct gtcaggccga tccgctgcgg ggcgggcggg
     4021 gttgcggcgt cggggacccg gccgcacccg gccagaagtt gaaggccggc cagccagggc
     4081 aggcaacgcc tcacagtcat cctacctcca gagtttgaaa gcggcgcatc ggcagtcccg
     4141 gaacgtgacg agggaatggc caaggcgcca ggggcacggc aaaatggccc ggtacggcca
     4201 atctgaccag accccggctc cgcggcggat cattatcttt gaggagctgt tggacaacca
     4261 ctggcgagca cgccgtagcg ggctcgcgtc tttgtgagtg ggagcgagtg tggaatcgaa
     4321 cgtgtttgag accgcgaacg ccggcttcgc acaggcaatg tacgaagagt tcctgcgcga
     4381 tccggctgca gtcgggccgg agtggcggcg gctgttcgaa agcggcgtag taggcgaaaa
     4441 acccaacggc gcagacggag cgcaccgtcg cacaggaacc gacttagcag cgcaagtcgc
     4501 cttcgaggta ggcgccgtcg agccggcccg gcggacaggc gacggtgaag ccactggttc
     4561 cgcggctccg cccgagccga tcagctcccc agcaccaggc cgggaagtag cccgccttcc
     4621 ggccggcgct tcgcccatca aaggcccggc agctcgcctg gtgacgaaca tgaccgagag
     4681 tctctcggtc ccgaccgcca ccacctttcg caccctcgcc gttgctaccc tcgaatcgcg
     4741 ccgtaaggag ctgaacgccc ggctgcagac cgccggacgg aaggaaaagc tgtccttcac
     4801 tcatctgatc gcgttcgcac taatccaggc cgcgaagcga catcctgtga tgggccacac
     4861 gctgctggtt caggacggtg cggtgtatag ggtgcagccc gagggaatcg ggctcggaat
     4921 cgcggtagac gtacagcgca aggacggaag tcgggggctg gtggtgcccg taatcaagcg
     4981 cgccgacact atggatttcg gcgagttcca cgcggcctac gaggccctag tggagaaggc
     5041 gcgggccaat cagctcatgc ccgacgactt cagcggctcc accattcagc tcaccaatcc
     5101 cggtggcctg ggcacagtcg cctcggtgcc ccgtctcatg gcgggacaag gcagcatcat
     5161 tgccgtgggt gcgatcggct acccggcaga gttctcctca gtgccggacg acaagctgcg
     5221 cgagctgggc gtcagcaagg tgatgacggc aagcagcacc tacgatcacc gcgtgattca
     5281 aggcgccgag tcgggggcgt ttctcgcgac cctcgaccac ttgctccagg gagaggatca
     5341 tttctacgag acggtggcgg agagtcttca gctatcagcg atcagctatc agcttgctcc
     5401 cgcagccacg cagcttccga aggcgcccga taccgtcgct ccggagatgc tataccacgt
     5461 cgcggcggcg atggcgttag tcaaggcatt ccggactcat gggcacctgg ctgcacacac
     5521 cgaccccttg ggcacggagc ctctcggcga tcccgcgctc gatctcggcc cgctcggact
     5581 cacgcccgag atcatggcag cgatcccgtc gaaagtgctg cgggtatatg tgccgggccg
     5641 gaccctggca gagtcgtacc cctacctgca acagacctac tgtggcacga tggctttcca
     5701 ggtggagcac atctccagcc accaggagcg cgtctggctg cgtgagaaga tcgagtcggg
     5761 cgcattccgg acgccgccct cggccgacga gaaaaagcgg ctgctggccc gcctcaccaa
     5821 agtggaggca ctggagcgat tcctacacaa ggcttacctg ggccacaagc gtttttcgat
     5881 cgagggggtc gacctactgg tcccgatgct cgacctgacg ctcgagctgg cggcaagcac
     5941 aggtgcccgg gaaatcgtca tcggcatggc gcaccgcggt cggctcaatg tactcgccca
     6001 catcatcggc cggccgtacg agacgatttt cgccgagttc gaaggtggtc gcttcgtgga
     6061 gggcaaccgg ctcacgccgg aaggtggcac aggtgacgtg aagtatcatc acggcgccga
     6121 gggcgcgttc ccgctccgca acggaaaatc catcgccgtc acgctgtcac ccaatccgag
     6181 ccacctcgaa ttcgtgtcgc cggtggtgga tggacgggct cgagcgacgc agacattgcg
     6241 gaagggtcgc tccgctcatc acgatcccac gattgccttg ccggtgctga ttcatggcga
     6301 cgcggcattc gcggcgcagg gcgtcgtcgc ggaaacgctg aatctgagtg ccttaccagg
     6361 gtacggcgtg ggcgggaccc tgcacattat caccaacaac cagatcgggt tcaccacaga
     6421 cactcaggac gcccgctcga cccgatacgc cagtgacctg gccaaggggt tcgacgctcc
     6481 aattattcac gtgaactcgg acgacgccga ggcatgtctc gccgcaatcc gattggccat
     6541 ggcctatcga gagaagttcc gtcgcgacgt gctcatcgac ctggtgggat atcgccggca
     6601 tgggcacaac gagagcgacg agccgactta cacccagcct gtgatgtacg agcggatcaa
     6661 gcagttgccg ccggttcgct cccgctacgc ggagcaactc gcggccgagg gcgtcgtttc
     6721 ggttgaagaa gctacccagc aggccgagag cgcgtaccaa cggctggtcg atgcgcagca
     6781 gcggttcaag gccagcctgg gcaaagggcc ggtccccgag cctcccaagc cgaccgggcc
     6841 ggggcacgag gtcgcgaccg ccgtagctcc ggaaacgctc acggcgctca acgagcagtt
     6901 actgacctgg ccgcagaact ttgctgtcaa tccgaagctc aagaaacagc tggaacggcg
     6961 gcgggtggcg gtgggtcctg aaggcggcat cgactgggcc cacgcagagg ccttggcgct
     7021 tgcatcgctg gtggtcgaag gcgtgccggt gcgcctcacc gggcaggata cggagcgcgg
     7081 cacgttcagc caacggcatc tggtcctgca cgacgtcaag accggtcagc gctttgcccc
     7141 gatccagcgg ctgcccggtg cgctcgctcc gctcgagctg cacaacagcc cgctctcgga
     7201 actggcgtct cttggcttcg agtatggcta cagtgcggag gcggccgaag cgctggtcct
     7261 gtgggaggca cagttcggcg acttcatcaa cggtggccag gtcattgtcg atcagttcct
     7321 gtcgtcagga ctctccaagt ggggactgac tactcgactc accctgctcc tgcctcacgg
     7381 gtacgaaggg cagggcccgg agcactcgag tgctcgattg gagcggttcc ttcagctgtg
     7441 cgccgagggc aacatccggg tcgccaaccc gacctcgccg gcccaatact tccacctgct
     7501 gcgacgccag gcccgacgga cccgccagcg gccgctcatc gtgatgaccc ccaagagcct
     7561 gctgcggcat ccgcaggcta gctcgcgcct cgaggaccta gccgagggaa cctggcgtcc
     7621 ggtgttcgac gatccctggg ccacggacga gagggcagca ggcgttactc gcctggtgtt
     7681 gtgcaccggc aagctttact acgagctgtt ggcggaagcc gagaagatga ccgcagagcg
     7741 gcccgcgctg atcaggttgg agcagctcta ttctttcccc tggtcagaat cacgtcaggt
     7801 cctcgcacgg tatccgaacg tccagcaact aatctgggct caggaggagc ctcgaaacat
     7861 gggagcctgg aactatctcg atcctaagct tcgggaattg ctgccggctg gtgccgagct
     7921 gcggtacgtc ggccgtccgg agcgagccag cccggcggag ggctatccgg ctgcgcacat
     7981 ggcggagcag agtcgcatcg tcgccgatgc gctgggagtc aagcagccac ggttgtcgcg
     8041 ggagactgtc gcgcccggtg gcgagggtca gggctgaaga ttgaaaacac gaggcgggtg
     8101 gcgatccgcc acccgccttt gctttttcag gcgccctttg ccaacagggg ccgctccgat
     8161 gccttacggg ccaactcttt gagaggctcg gagagatggt cgccgtaatg aagcaggaac
     8221 acaccgcgcg accggcgcat catcagaaac gcgaggagcg gggtgtctgc tttggccgcg
     8281 ttgcaggtcg aacacgccaa gacaagattg tccgggcggt cgtacgcggt ttgaccccgg
     8341 cgagggcgaa catggtccag cgtaatggct tccggggacg tactcgtccc acagtaagcg
     8401 cacacgggtc cgtgttgcgc gagcagccaa tcacgatggg attgaattga acggccgggg
     8461 gaacggcgaa gcgtctggag ggatgttgca gaggaacgat gtcgagctct ggatctcaca
     8521 aatcacaagg ttaaaggttg aactgagggg cttaagggaa gccccatcgc acaggaaaac
     8581 atagcaagtt aagagccaaa ctgcatgtct ctgctctcga gccggataac caaacccggt
     8641 ttcacaatca cagacacagc cggcacccgc cggttagcag ttggggttcg agcagctctg
     8701 gcggcgtggg cggtctgcct gccacttgcc ttgagcgctt gccgaaccgc cgaggcaggc
     8761 tcggacgtcg cagctaagcg catgattggc aatgtattag atcggtacac caaggcgtac
     8821 cgggacaacg accccgatgc catttcaaag ctgtacgcac aggaggcggt actgatgccg
     8881 ccaggccatg agctggtcga aggccgagac agtgtcaggg cgttctgggg ccgaggaatg
     8941 gagccgggct tcaggatgac gaccgtaagg gtcgaggttt cggggagcac tgcctacgtc
     9001 gttggacggt actacgtgcc gccggatgac gatgacgacg cggaaaccgg caagtatctc
     9061 atcgctctcc ggaaagagcc cgacggcaac tggcgcatta ccgctgatat ctggaacgac
     9121 gacgaatcag atgaggagcc tgactctacc agcgacacca cgcgtcaatc ggtcgccctg
     9181 cgggcaagcg cgcgcctcag gtccgggcta ccatgaccca gagggcgtag aagagaagcg
     9241 ctgtcgccgt ccagccgacg atactccacc ggagaaacag tgcgcgcagg gtggcgtcgt
     9301 cacccggtgc cgcgcgttcg aggcgccact ggtcgggcag cagcactgcg agccagaggc
     9361 ccgtgctcac gatgagggcg attactcctt gcagcaacca gggcgttgcc gtgagcgagt
     9421 acccccgggc ccgggccagc agaatccccg agattaccag catcatgccg ccccccaccg
     9481 tgaacacgag gtccgtccag agaatcgccc gggcaaccac acgaaagggc acaactcggc
     9541 gcgcccggaa cagaaaggct gcccagaatc cggtgatggt cacgttgccg agtagaagaa
     9601 cggctcccac gacgtgaacc agccgcagga aggaaacctc acccattccg tggctcgaac
     9661 cgcccccgca gcaggccgac cgcgtgccgc acccgatcca tccgtttgtt gatggagagc
     9721 cggcccgcca ctaccgtccg aaacatgctc acgcgctcgg tacgcaggtc ggcgtcgagt
     9781 gcgtcgatca cgctcaggag atccgcctcg aacaagggca tcagggcacg ccgatcggcg
     9841 tagtgggatg gatcgagtag ccagccccgg ccgatccggg cgaatgcctc gggcagctgt
     9901 tcgctcagat ggacgtagcg gaacgcgacc
//
LOCUS       DASRJQ010000610         5079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3333483, whole genome shotgun sequence.
ACCESSION   DASRJQ010000610 DASRJQ010000000
VERSION     DASRJQ010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5079
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3333483"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1600
                     /locus_tag="VFB89_13835"
     CDS             <1..1600
                     /locus_tag="VFB89_13835"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HZM28423.1"
                     /translation="YSVENLRALGRSHESLEALVEELLRLRVGPYRNPLEEHHQELSD
                     PAAYPGAVELADRLEAAMQSGAEVWLEPDRGVEIALQGMLRKAGLANVSRLVPGQSSP
                     AAAFTLRPADAVAGRWPMRLFKALQLMHVRSTRNQLTDYVAFDLETTDLDVSGCEIVE
                     LAGVRVRDGTPVAEFRELVRCARPVSAKARAIHGYSDEDLRLAAPFAEVWSRFRRFVG
                     SDLLVAHNGQRFDVPVLRRLAAAYGGTDDLVFFDTLPLARSVLDESARLEDLASRFQV
                     PLGRAHHALDDARALAGVLAHLEQLKLVRGRKAALAQVLGYLAMALALDQAPAPTPEE
                     RLLREIALPHALGRYGDCLEQYAAEFEAGAPGAPPLEELIERLGGRRIMERLRAERTP
                     AERYPEAVARLSALVESSKGDLLRERIDSLLDLVALSSSEGVETNPARVSLLTLHSTK
                     GLEFSRVYVVGVEDNQMPGWHALEENREQEIQEARRLLYVGMTRAKDRLVLTRTERRG
                     GRSSGGDLFLRDAGLVAVVPEPTG"
     gene            1681..3105
                     /locus_tag="VFB89_13840"
     CDS             1681..3105
                     /locus_tag="VFB89_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZM28424.1"
                     /translation="MRFSPMLYSLKAWILVAGVVCSSARPVGAQTAAGSIIDRYRDPA
                     NRLIEAALADTTAYRRLEYLTDKIGNRPSGSQALERAIDWVLATMRADGLSQVRGEPV
                     MVPHWLRGAESAELVAPRWLALHVLGLGGSVATPAKGITAPVLVVESFDELAQRAAEA
                     RGKIVLFDVPMAADEPPFVAYDEAVQYRVDGASAGARVGALAVLIRSVASFSIQSPHT
                     GIMRYDSTVARIPAAALSVEDAELLHRLQRRGDSVVIRLRMDDRMLPEAASRNAIAEL
                     SGTERPDEVVVLGGHIDSWDVGQGAVDDGGGAMAAWEAVRLMKELGLRPKRTVRVVLW
                     TSEETGAVGGKAYRDRHQAELTRHILAIETDNGVFAPTGFALRGSDSAAAVAREIGGL
                     LERVGAATIEREKEGPEADAEPLANDGVPAMGLRVDASRYFWYHHSAGDTFDKIVPAE
                     LQRCVAALAVMAYVAADLPDPLPR"
     gene            complement(3106..4263)
                     /locus_tag="VFB89_13845"
     CDS             complement(3106..4263)
                     /locus_tag="VFB89_13845"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HZM28425.1"
                     /translation="MTERLYYTDSYLTDFEARVTERGDDGRRIYLDRTAFYPTSGGQP
                     HDMGLLGEAKVLDVVDEGERIAHLLSQPLPADRVAGRVDWERRFDHMQQHTGQHLLSA
                     VLADLYRFATVSVHFGQETSTLDLDVAEVTPGQLAEAEMRANAIATENRPVTVGFEDA
                     ALAANLRKPSDRQGPLRVVTIADLDRSACGGTHVRATGEIGAILIRRTERVRKSARLE
                     FVCGRRAIRAARADSDLLSRIAGSFSAAPADLPALIDAQRAALKEAASTRRELDSRLD
                     ALRARELYADAAPGADGVRRIIVRDESAVERLRFLGQAVASLSQVVFIGAARTPPGLV
                     VTTSDDSGRDAAAWLRTVLERVGGRGGGNARLAQGTVPADTLDAALDMLTE"
     gene            complement(4347..>5079)
                     /locus_tag="VFB89_13850"
     CDS             complement(4347..>5079)
                     /locus_tag="VFB89_13850"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZM28426.1"
                     /translation="LTALIVAGMPLWVALFERVAPGGKPLSLARVTGLLLGFGGLALL
                     VTGSTPESAGCVSASTPCGAASDVPLFYLLTVPLASISWALGSVISRRVRLPANQVLT
                     SAMNMMAGGAVALLIALVTGELRGFSPAQLSAGSLAAWAYLVVFGSMLAFTCFTWLVS
                     VVEPTRVATYAYVNPVVALVLGAWLAGEPVGSRILLATPVILVGLALVLRSGPATGAS
                     GSESSAARRAVSRPSDPGQPADTAA"
BASE COUNT          907 a         1651 c         1715 g          806 t
ORIGIN      
        1 ttattcggtc gaaaacctgc gggcgctggg ccgctcgcat gagtctctcg aggcgctggt
       61 cgaggagctg ctgcggctcc gggttggacc gtatcgcaac ccactggaag agcaccacca
      121 ggagctgagt gatccggcag catatcccgg cgcggtagag ctggcggacc ggctggaagc
      181 ggcgatgcag tcgggagcgg aggtgtggct cgagcccgat cgcggcgtcg agatcgcact
      241 ccaggggatg ctccgaaagg ccggcctggc aaacgtgagc cggctggtgc ccggtcagtc
      301 ttcccccgcc gcagcgttca cgctccgacc agctgatgcc gtagccggtc gctggcccat
      361 gcggctgttc aaggcgcttc agctgatgca cgtgcggagc acacgaaatc agctgaccga
      421 ttacgtcgca ttcgatctgg agacgaccga tctcgacgtg agtggatgcg agattgtcga
      481 gctggctgga gtccgggttc gcgacggaac gccggtggcg gaattccgcg agctggtccg
      541 atgcgcccgg ccggtgagcg caaaggcacg agccatccac ggttacagtg acgaggattt
      601 gcgactggcg gcgccatttg ccgaggtctg gtcccgattc cgccgattcg tgggcagcga
      661 tctcctggtc gcccataacg gacagcggtt cgatgttccg gtcttgcgtc ggctcgcggc
      721 cgcgtacggc gggaccgacg acctcgtgtt cttcgacact ctgccactcg cccgatcggt
      781 gctggacgag agcgcacggc tggaagacct ggcgtcgcga ttccaggttc cgctgggccg
      841 ggctcatcac gcattggacg acgcgcgagc cctggccggc gtgcttgcgc acctcgaaca
      901 gctcaagctg gtgcgcgggc gcaaggccgc gctggcgcaa gtgctgggtt acctcgcgat
      961 ggcgctggct ctggatcagg ccccggcccc cacgccggaa gagcgcctgc tcagggagat
     1021 cgcactgccg catgcactcg gacgctacgg ggattgcctc gagcaatacg ctgccgagtt
     1081 cgaggcgggc gctcccggag cccctccgct cgaggagttg atcgaacggc ttggtggtcg
     1141 ccgcatcatg gagcgcttgc gggcagagag aacgccggcc gagcgctatc cggaggccgt
     1201 ggctcgtctc tcggcgctgg tggagtcgag caagggcgac ttgttgcgcg agcgtatcga
     1261 cagcctgctg gatctggtgg ccctctcgag cagtgagggg gtcgagacca atccggcgcg
     1321 ggtcagcctc ctcaccctcc actcgaccaa aggcctcgaa ttctcccggg tttacgtcgt
     1381 gggcgtcgag gacaaccaga tgcccggctg gcacgcactc gaggagaatc gggaacagga
     1441 aatccaggag gctcgacgac tcctctacgt cggaatgacc agggccaaag atcgcctggt
     1501 gctcacccgc acagagcgcc ggggagggcg ttcatccggg ggcgacctgt tcctgcgcga
     1561 tgctggactg gtcgcagtgg tgccagaacc tacgggctga ccatccctcc gggcccacga
     1621 cacttggccc ggccctatcc cgtacggttc aaacctctcc gcatccgcga gcacgctcgc
     1681 atgagatttt cgccaatgtt gtacagcctg aaagcatgga ttctggtggc cggcgtagtc
     1741 tgctcctctg cgcggccagt cggcgcccag accgcagccg gatcgattat cgatcgctac
     1801 cgcgatccgg ccaaccggct catcgaggct gcgctcgcgg acaccacggc gtatcggagg
     1861 ctggaatacc tgaccgacaa aattggaaac cgccccagcg gatcccaggc actcgagcgc
     1921 gcaatcgact gggtgcttgc gaccatgcgg gcggacggat tgtcgcaggt gcggggcgaa
     1981 ccggtcatgg tgccccattg gctgcggggc gctgaatccg cggaacttgt agcgccgcgg
     2041 tggctcgcgc tccacgtcct cggcctgggt ggaagcgtcg ccacgccggc caaggggatt
     2101 acggcgcccg tgctggtggt cgagagcttc gacgagctcg cccagcgcgc cgctgaggca
     2161 agagggaaaa tcgtgttgtt cgacgttccc atggctgccg acgagcctcc gtttgtcgcc
     2221 tacgatgaag ctgtgcagta tcgagtcgac ggcgccagcg ccggtgcgcg ggtcggtgca
     2281 ctggcagtgc tgatccgttc ggtggcctcg ttctcgattc agagtccgca caccggcatc
     2341 atgcgttacg actcgacggt cgctcggatt cctgcggctg cactcagcgt cgaggacgcc
     2401 gagctgctgc atcgcctcca acgtcggggg gactccgtag tgattcgctt gaggatggac
     2461 gaccgcatgt taccagaggc ggcgtctcgc aatgcgatcg cggagctttc gggcacggag
     2521 cggcccgatg aagtagtggt tctgggcggc cacatcgatt cctgggatgt tggccagggt
     2581 gctgtcgatg atggcggcgg agcgatggct gcctgggaag cggtccggct gatgaaggag
     2641 cttggcctcc ggccaaagcg aacagtccga gtggttctct ggaccagcga agagaccggt
     2701 gcggtcggcg ggaaagcata ccgcgatcgg catcaggctg aactgacccg ccacatcctg
     2761 gccatcgaaa cagacaacgg agtgttcgcg ccaacgggct ttgcgctgcg gggatcggac
     2821 tccgctgccg ccgtcgcacg ggagatcggt gggctgctcg aacgagtggg cgctgcgacc
     2881 atcgagcgag agaaagaggg cccggaggca gacgccgagc cgctggccaa tgatggcgtg
     2941 cccgcgatgg gacttcgggt ggacgcctcg cgatacttct ggtatcacca ctccgccggc
     3001 gacacattcg acaagatcgt gccggccgag ctacaacgat gcgttgcggc actcgcggtc
     3061 atggcatacg tcgcggccga ccttcccgac ccattgcccc gctgactact cggtcaacat
     3121 atccagcgcc gcatccagcg tatcggcagg cacagtgccc tgtgccagcc gcgcattgcc
     3181 tccgcctcga ccgccaaccc gttcgagaac cgtccgcagc cacgcggcag catcccgacc
     3241 cgaatcgtcc gacgtagtga ctaccaggcc aggtggagtc ctggcagccc caatgaacac
     3301 gacttgggac agtgacgcca ccgcctgccc cagaaagcgc agacgttcga cggcggactc
     3361 gtcgcgcaca atgatccggc gaacgccatc agcacccgga gctgcatcgg catagagttc
     3421 ccgggcccgc aacgcgtcga gcctggaatc aagctcccga cgggtagatg ccgcttcctt
     3481 gagtgcagcc cgttgcgcat cgatcaaggc cggcaggtcg gccggagcgg cggagaacga
     3541 accggcaatg cgcgagagca gatcgctgtc ggcgcgagcc gcccgaatcg cccggcgtcc
     3601 gcagacgaac tcgaggcggg ccgacttccg cacgcgctcc gtcctgcgga tcagaatggc
     3661 accgatctca ccggtggcgc gaacgtgggt ccccccacag gcactgcgat cgagatccgc
     3721 tatggtgacc actcgcagcg ggccctggcg gtcggatggc tttctcaggt tggcggcgag
     3781 tgcggcgtcc tcgaagccga cggttacggg gcgattctct gtagcgatcg cattggcccg
     3841 catttcggct tcggcgagct gcccaggcgt gacttcggcc acgtcgaggt cgagggtcga
     3901 ggtctcctga ccgaagtgca cgcttacggt tgcgaagcga tacaggtcgg ccagcacggc
     3961 ggaaagcaaa tgctggccgg tgtgctgctg catgtggtcg aagcgccgtt cccaatccac
     4021 cctgcccgct acgcggtcgg ccggcagcgg ttgactcagg agatgagcta tccgctctcc
     4081 ttcgtcgaca acgtcgagca ccttcgcctc gcccagcagg cccatgtcgt gtggctgacc
     4141 accggacgtg gggtagaacg ccgtccgatc gaggtagatt cgccggccgt cgtcgccacg
     4201 ctcggtaacg cgcgcctcga agtcagtgag ataggagtcg gtgtagtaga gccgctcggt
     4261 cacggcgaat gtctaccatc ggctgatggc gagcggaagg ctacgcgcgc tccggcgcaa
     4321 taagatcgag cgcagcgaag gcgcgatcag gccgcagtgt cggcgggctg acccggatcg
     4381 gacggacgag aaacggcgcg ccgcgcggca gacgattcgc tgccggacgc acccgtcgca
     4441 gggccggatc gcagcacgag cgcaaggccg accagaatga ccggtgtcgc gaggagaatg
     4501 cgggagccga ccggctcgcc tgcgagccag gcacctagta ccagagcgac caccgggttc
     4561 acgtaggcgt aggtggctac ccgggtcggc tcaacgacgc tcactagcca ggtgaagcag
     4621 gtgaatgcca gcatcgagcc gaaaaccacc aggtaggccc atgctgccag cgagcccgcc
     4681 gacagctgcg ccgggctgaa gccccgcaac tcaccagtga ccagggcaat caggagcgcc
     4741 acggcgccac cggccatcat gttcatcgcc gacgtcagca cctgattggc cggcaagcgc
     4801 acccgccggg agatcaccga accgagagcc caactgatcg acgccagcgg taccgtgagc
     4861 aggtagaaca gcggcacgtc cgaagccgcc ccgcacggtg tcgatgcgct gacgcacccg
     4921 gcgctctccg gcgtggagcc ggtcaccagc aatgccagac cgccgaaacc caagagaagt
     4981 cccgtcaccc gcgcgagcga aagcggcttc ccgcccgggg ccacccgctc gaacaaagcc
     5041 acccagagcg gcattccggc cacgatcagc gccgtcagg
//
LOCUS       DASRJQ010000611         2167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3808203, whole genome shotgun sequence.
ACCESSION   DASRJQ010000611 DASRJQ010000000
VERSION     DASRJQ010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3808203"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="VFB89_13855"
     CDS             complement(<1..943)
                     /locus_tag="VFB89_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28427.1"
                     /translation="MADRILHFPDDVSVGFFYAYVGSTAVKSGDARGRVSVPERPDVH
                     LSLSCPIRGLGQLRPDDIATIAIQKKSAKDVDFSRLSHLTGLLELNASKSHDVGDAGL
                     AAIAPLRRLRSLDLYASAVTDAGLVHLSGMVDLEHLNLGATRVKGPGLAHLVGLQRLS
                     YLTVDRIGDDGVPHLLRLRGLDRLVLRETRLTEDGVARLRAGFPGIRELYVEPSEKYS
                     RARAWAREGVLAVLAGRLRSGAAWDLPDTALRAMLPVGSRIAEIWREGSVPRAVGWSL
                     DDLDRIASTLGTLGPGYDLRIVTPMGMDFWIPWLRARR"
     gene            complement(955..1314)
                     /locus_tag="VFB89_13860"
     CDS             complement(955..1314)
                     /locus_tag="VFB89_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28428.1"
                     /translation="MRGRELSLQDVNDVGGDGRSVSPRASPVRVWRLGRHLLDDLSDE
                     FLGAPGAAGLALARPDKLVLVEAVHQHFDADVKLGDQVARRMAATISRRFGVFRGAMP
                     SLSDLSRRGAVAFWWAL"
     gene            complement(1265..1645)
                     /locus_tag="VFB89_13865"
     CDS             complement(1265..1645)
                     /locus_tag="VFB89_13865"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HZM28429.1"
                     /translation="MRKTRFMEWVDEQVDSDARLASEVSRLLSEMRLEQELVALRERR
                     GLSQREAAKLIHATQPYVAKLESGRIQNLGVATLVKYAHALGGRLTIDIKAKRAARRS
                     LGAGTRPRSARPRVVAAGRKRRRR"
     gene            complement(1642..2016)
                     /locus_tag="VFB89_13870"
     CDS             complement(1642..2016)
                     /locus_tag="VFB89_13870"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HZM28430.1"
                     /translation="MPAKTRWTVDAYATEKGESPAWSFMKNLQGRDKAEAIALVKLLE
                     EQGNALRRPQSGTLGQGLFELRGKQIRIFYMFLPNRVAVLLDGEIKKQGAVPLATVRR
                     VREYQALVMKRQSAIVSREKKR"
BASE COUNT          390 a          771 c          654 g          352 t
ORIGIN      
        1 gccgccgcgc cctgagccag ggaatccaga agtccatgcc catcggggtc acgatccgca
       61 ggtcataccc aggcccgagc gtcccaagcg tgctcgcgat ccggtccagg tcgtccagcg
      121 accagcccac cgctcgcggc accgaccctt ccctccagat ctcggcgatc cggcttccaa
      181 cggggagcat cgcccgcagt gccgtgtcgg gaagatccca agcggccccg ctgcgcagcc
      241 gccccgccag gaccgccagc acgccttcgc gggcccacgc acgcgcgcgg ctgtacttct
      301 ccgagggctc cacgtagagc tcgcggatgc cgggaaagcc ggcacgcaat cgtgcgactc
      361 cgtcctcggt cagccgcgtt tcgcgcagga ccagccggtc cagccctcgg agtcgcagga
      421 ggtgcgggac cccgtcgtcg ccgatccggt cgaccgtgag gtacgagagc cgttgtagcc
      481 ccaccaggtg cgctagaccc ggacccttca cgcgcgtagc ccccaggttg aggtgctcca
      541 gatccaccat cccggaaagg tggacgagac ccgcatccgt gaccgcgctc gcgtagagat
      601 ccagcgatcg cagccgccgc aatggagcga tcgcggccag gcccgcatcg ccgacgtcat
      661 gtgacttcga tgcgttgagc tccaataggc cggtgaggtg ggacagccgg ctgaagtcga
      721 cgtctttggc ggacttcttc tggattgcga tcgtcgcgat gtcgtccggg cgcaactgtc
      781 ccagtccgcg gatcgggcag ctgagcgaca ggtggacgtc tggtcgttcc ggcacgctca
      841 cgcggccacg cgcgtcaccg ctcttgaccg cggtcgagcc cacatacgca tagaagaagc
      901 ccacggacac gtcatcaggg aaatgaagaa tccgatcggc catcaattca cctctcagag
      961 agcccaccag aatgccacgg cgcccctccg cgacaggtcc gaaagtgacg gcattgcccc
     1021 gcggaaaacc ccgaagcgtc tggaaatcgt tgctgccatg cgccgggcga cctgatcacc
     1081 cagtttcaca tctgcgtcga agtgctggtg tacagcctcg accagaacaa gcttgtctgg
     1141 gcgggccaga gcaagaccag cagcccccgg agcgccgaga aactcgtccg agaggtcgtc
     1201 aagaaggtgg cggccgagac gccagacccg gaccggcgat gcacgtggag aaaccgaccg
     1261 cccgtcaccg ccgacgtcgt ttacgtcctg cagcgacaac tcgcggcctc gcacttcgag
     1321 gcctcgtccc ggcgcctagg gagcgccgcg ccgcacgctt ggccttgata tcgatcgtga
     1381 ggcgcccccc gagggcgtgt gcgtacttga ccagagtagc gacgccgagg ttctggatgc
     1441 gccccgattc gagcttggcc acgtacggct gggtggcgtg aatcagcttc gccgcctccc
     1501 gttgagacaa gccgcgtcgt tcgcgtagcg caaccagctc ctgctcgagt ctcatctcgc
     1561 tgagcaagcg gctcacttcg gacgcgagcc gcgcatccga gtcgacctgc tcgtcgaccc
     1621 actccatgaa ccgcgtcttt ctcaccgctt cttctcccga ctcacgatcg ccgactgtcg
     1681 cttcatcacc agggcctgat actcccgcac gcgacgcacc gtcgccagtg ggaccgcgcc
     1741 ctgtttcttg atctcgccat ccagcagcac agcaactcga ttcggcagga acatgtagaa
     1801 gatccgaatc tgctttccgc ggagctcgaa gaggccctga cccagcgtcc ccgactgagg
     1861 ccgccgtagc gcgttgccct gctcctcgag cagcttcacg agcgcgatcg cttcggcttt
     1921 gtccctcccc tgcagattct tcatgaacga ccaggcgggc gattcgccct tctccgtcgc
     1981 gtaggcatcg acagtccagc gtgtcttcgc aggcatggcc agtataactc aaaggttatc
     2041 gggagccgca gggctggcat gtccgcggaa ggccggaagg gcaggcggac gtcaacgaga
     2101 tagtcgatcg gctaccggtc tggagaggtc cgaatgctcc cccaccccca accgatccgc
     2161 cagctcc
//
LOCUS       DASRJQ010000612         4989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1835102, whole genome shotgun sequence.
ACCESSION   DASRJQ010000612 DASRJQ010000000
VERSION     DASRJQ010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1835102"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2063
                     /locus_tag="VFB89_13875"
     CDS             <1..2063
                     /locus_tag="VFB89_13875"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZM28431.1"
                     /translation="LITGTLQNLPGDVGIRTAFGPRIFIPAQFLDETRLLRFGSQAER
                     QAFIKLREGASAQSIAQKLRPRLNAQHVRVRTVADDRENLNNLLTRLSRYLGLVALVA
                     LLLGGVGVGSAVVVFVRRKLETIAVLRCLGATAGQVFAIYLTQAAALGLAGSLLGATA
                     GVLVQQLLPVLLHGLLPVDVHAAIVPGAVALAVGLGAWTALIFALLPLLGVRLVSPLA
                     AIRRPYEAVPVPRDRWRWPAIAALVASVVGLASLEVGNGRTGLVFTGTIGLALGVLWL
                     AAWLLVRGLRRWFPATWPYVWRQGLANLFRPANQTVAVVLALGFGAFLLSTLFLVQHN
                     LRRGLELTGGPRRPNLLLLDIQSDQAQAVAASLRARGLAEYGPVPLVPMRIRSIKGRP
                     VEELLADTGGRGRGEPANAWALRREYRSTYRDTLVASERLVAGRWWSAATSSDTAKAV
                     SISVEQDLASELGVGLGDEIVWDVQGVALPSRVTSLREVNWARFEPNFFVVFAPGHLE
                     QAPQMLVALTRIDDAATRGSFQRELAERFSNVTTVDLALVQQQLEQLVDRVALAIRFM
                     AAFSLAVGGLVLVGAIATTRLQRLRESLLLRTLGATRRQVLRIALAEYLALGLLASVT
                     AVVLATAAGWGLTRWYFDGRFWIPFLPLLGLVAGIATVSVTVGLWTTREALSGTPLEV
                     LRAE"
     gene            2133..3044
                     /locus_tag="VFB89_13880"
     CDS             2133..3044
                     /locus_tag="VFB89_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase"
                     /protein_id="HZM28432.1"
                     /translation="MLAIDLTGKRALIAGVSDDGGFGFAIAKALAQAGASICLGSWPP
                     ALGIFHKLLERGKMEESLRLSDGRKLEFERIYPLDAAFDRLDDASPDIRDNKRYKEQG
                     DFSIEGMAARIRDDFGDRGLDIVVHSLANAPEVKNPLLETSRAGYLSAVSVSAYSNIS
                     LVRHLAPMMRPGGAFLSLTYIAGERVIPGYGGGMSSAKAALEADTRTLAFEAGRKWGV
                     RVNAISAGPWASRAASAIGFIHTMIAYTTANSPLARAIDAAEVGHTAAFLLSPLAAAI
                     TGSVIYVDNGYHTMGMAVAPPASGTPE"
     gene            3046..3834
                     /locus_tag="VFB89_13885"
     CDS             3046..3834
                     /locus_tag="VFB89_13885"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZM28433.1"
                     /translation="MSVPELISRADGPAWRLTINRPGRRNALTPELAAAAAREIDEVC
                     ASQSARVLLLDGAGGHFCSGLDLRWLAELGPNPIRGDIRDGLERFQSVILAIVKASLP
                     VVALVRGTAAGFGVDLAVACDTRLAGTSATFTSAFSRMGLVPDGGSTYGLPRLIGDGA
                     ALRFLLASETVDAAAALRTGLVDEVVDDAGLDARAMSLAQALAANAPLSLSSIKRLCR
                     AEELAGLERALRAEGEAQIEAFLGLEFQRRLAAFLARTSAQEPA"
     gene            3831..4646
                     /locus_tag="VFB89_13890"
     CDS             3831..4646
                     /locus_tag="VFB89_13890"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZM28434.1"
                     /translation="MSPGPIFAPDLLAGRVALVTGGGTGIGFGIATCLASAGAAVAIA
                     SRKPDHLEPALAQLRTSSPRAMSVEVNVREADSVGRMVERVTNELGRLDILVNNAAGN
                     FYAPSADLSPNAWRAVVETDLYGTFYCCQAAYPAFREAGGGRIISVSMTLHYRGWPQM
                     AHATAAKAGVDALTRTLALEWASDNIRVNAVAPGPIPTEGVKKAFTPPGRDAPDFFQM
                     EAYAKRSIPAGRWGAPEDIGHMVTYLAGPAGDWITGAVFVIDGGHWLVPTERR"
     gene            4646..>4989
                     /locus_tag="VFB89_13895"
     CDS             4646..>4989
                     /locus_tag="VFB89_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HZM28435.1"
                     /translation="MERVFFATGALSGFIAVAAGAFGAHGLRQHLAPEQLSVFETAAR
                     YQMYHALGLLAVGWAAARWPGSPATWAGWLFIVGTLIFSGSLYTLALTGLGWLGAITP
                     IGGLCFLGGWLCL"
BASE COUNT          755 a         1591 c         1650 g          993 t
ORIGIN      
        1 ttctcatcac gggcaccctg caaaatctcc ccggcgacgt gggcatcaga accgccttcg
       61 gtccccggat tttcatcccc gcccagttcc ttgatgaaac ccgactactc cggttcggct
      121 cgcaggccga acgacaggcc ttcatcaagc tgcgcgaggg agcttcggcc cagtcgatcg
      181 cgcagaagct ccggccccgg ctcaacgctc agcacgttcg ggtccgaacc gtggccgacg
      241 accgcgaaaa tctgaacaac ctgctcacgc gattgtctcg ctacctgggt ctcgtcgcgt
      301 tggtcgctct cctactcggc ggcgtcggtg tcgggagcgc cgtggtcgta ttcgtgcgcc
      361 gcaagcttga aaccatcgcc gtactccgct gcctcggcgc cactgccggg caggtgtttg
      421 cgatttatct gactcaggca gcggcccttg gactggcagg gagccttctg ggtgccacgg
      481 ctggtgtgct ggttcagcag ctgttgccgg tgctgttgca cggattgctg ccagtggatg
      541 tgcatgctgc cattgttccg ggtgccgtcg cgctggccgt cggattgggc gcgtggaccg
      601 cgctcatctt tgccttgctc cctctgcttg gcgtcagact ggtatcgcca ctcgcagcca
      661 ttcggcggcc atatgaagcc gtgcccgtcc cgcgggaccg atggcggtgg cccgcgatcg
      721 cggcgttggt cgcgagcgtc gtcgggctcg cctcattgga agtcggtaat gggcgcaccg
      781 ggcttgtatt caccggtact atcgggcttg ccctcggcgt gctctggttg gccgcctggc
      841 tcctcgtgcg gggactgcgc cgctggttcc ctgcaacctg gccctatgtc tggcgtcagg
      901 gactcgccaa tctgttccgc ccggcgaatc agaccgtcgc tgtcgtgctt gcgctcggct
      961 tcggcgcctt tcttctcagc actttgttcc tcgtgcagca caatctccgc cgcggactcg
     1021 agctcaccgg tgggccgcgc cggccgaatt tgctgctgct cgacatccag agcgatcaag
     1081 cgcaggccgt cgcggcatct ctacgcgcac gtggactggc ggaatacggc ccggtgcctc
     1141 tggtgccgat gcgcatccga tcaataaaag gccggccggt ggaggagttg ctggcggaca
     1201 cagggggtcg tggccgtggc gagccggcca atgcctgggc cttgagacgc gagtaccgga
     1261 gcacgtatcg cgacactctg gtcgcgtccg agcgattggt agccggtcgt tggtggtcgg
     1321 ccgcaacgtc ctcagacacg gcaaaggccg tgtccatttc cgtcgagcag gatctggcca
     1381 gcgagctggg cgtcggcctg ggcgacgaga ttgtgtggga cgtgcagggc gtggcactgc
     1441 cctcgcgggt taccagcctg cgggaagtaa attgggcccg tttcgagcca aacttttttg
     1501 tcgtgtttgc gcccggtcat ctggagcagg cgcctcaaat gctggtggcg ctgacccgga
     1561 tcgacgacgc cgcgacccgc ggaagctttc aacgtgagtt ggcagaacga ttcagcaatg
     1621 tgacgacggt ggacttggct ctggttcagc aacagctcga gcagttggtc gatcgggtgg
     1681 cgctggcgat tcggtttatg gcggcattta gcctggccgt gggtggcctg gttctggtcg
     1741 gcgcgattgc gaccaccaga ttgcagcggc tgcgcgaatc gctgctcctg cgaaccctgg
     1801 gggccacccg gcgccaggtg ctgcgaatcg cactggcgga gtatctggcg ctcggactgc
     1861 tggcttcggt cacggccgtc gtgcttgcga cggccgcagg ttgggggctc acccgttggt
     1921 acttcgacgg gcgcttctgg atccctttcc tccccctgtt aggcttggtg gccggaatcg
     1981 ctacagtcag cgtgactgtc gggctttgga ccacgcgcga agcgctcagc gggactccgc
     2041 tcgaggtgtt gcgggccgag tgaagctgcc tactattcca acgccccctg tgccaatgat
     2101 atggagctcg gctcttttgc tttgggagtg acatgctcgc catcgatctc accggcaagc
     2161 gggcgctcat tgccggcgtt tcggacgacg gcggcttcgg gtttgccatc gcaaaagctc
     2221 tcgcccaagc cggcgcgtcg atctgcttag gcagttggcc tccagcgctc ggcatctttc
     2281 ataaactgct cgagcgcgga aaaatggaag agtcgctgcg actttccgac ggccggaagc
     2341 tggagttcga gcggatctac ccactcgacg ccgctttcga ccgcctggat gatgcttctc
     2401 cggacatacg tgacaacaaa cggtacaagg agcagggaga cttcagcatc gaggggatgg
     2461 cggcgcggat tcgcgacgat ttcggcgacc gcggtctcga cattgtggtt cacagtctgg
     2521 ctaacgcgcc cgaggtaaag aatccactcc tggaaacgag ccgggccgga tatctctccg
     2581 cggtgagtgt aagcgcctac agcaacatct cgctcgttcg tcatctcgcg ccgatgatgc
     2641 gtccgggcgg cgcgttcctg tcactcacct acattgcggg cgagcgggtc atcccgggct
     2701 acggcggcgg catgtcgagt gccaaagcgg cgctcgaggc ggatacccgg acgcttgcgt
     2761 tcgaggcagg ccggaaatgg ggtgttcggg tcaacgcgat ttcggccggg ccatgggcct
     2821 cccgcgccgc ctccgcaatc gggttcattc acacgatgat cgcatatacc actgccaact
     2881 cacccttggc gcgcgcgatc gacgctgccg aggtgggaca cacagccgcg tttcttctca
     2941 gtccactcgc ggcggccatc acgggcagcg tgatctacgt ggacaacggc taccacacga
     3001 tgggaatggc cgtcgcgccc cccgcgtcag gtacgccgga ataacatgag cgttcccgag
     3061 ctgataagcc gcgcggatgg cccggcctgg cggctcacga tcaatcgtcc cggccgtcgc
     3121 aacgcgctta cccccgagct cgctgcggcc gccgcacggg aaatcgacga ggtctgcgcc
     3181 agccagtcag cccgcgtgct cctgctcgac ggtgccggcg gccacttctg ctcgggactc
     3241 gatcttcgct ggctggcgga gctcggaccc aacccgattc gcggtgacat tcgcgacgga
     3301 ctggaacgtt ttcagtccgt catcctggcg atcgtcaagg cctcgctgcc ggtggtcgcg
     3361 ctggtgcgcg ggacggctgc aggcttcggt gtggacctcg ccgtcgcgtg cgacacgcgt
     3421 ctggcgggca ccagcgcaac ctttacatcg gcattctccc gcatgggatt ggtgcccgac
     3481 ggcgggtcca cctacggtct gcctcgcctg atcggcgacg gggcagcgct ccgattcctg
     3541 ctcgccagcg agaccgtcga tgccgcggcg gcgcttcgca ccggccttgt ggatgaggtc
     3601 gtggacgatg ccggactcga cgcccgcgcg atgagcctgg cccaggcgct ggcagccaat
     3661 gctccgctga gcctctcgtc catcaaacgt ctctgccggg cggaagagct ggccggactc
     3721 gaacgcgccc tgcgagccga gggtgaagca cagatcgagg cctttctcgg cctggagttt
     3781 cagcgacggc tggcggcgtt tctggcacgg accagcgcgc aggagcctgc ttgagtcccg
     3841 ggccgatctt cgctcccgac ctgctggccg gccgcgtagc cctggtcacc ggcggcggca
     3901 cgggcatcgg ctttggcatt gcgacgtgcc tcgcatcggc aggcgcggca gttgccatcg
     3961 cgagccggaa gccggaccac cttgaaccgg ctctggccca gctgcgcacc tcgagtccac
     4021 gggcaatgtc ggtagaggtc aatgtccgcg aggccgactc cgtcggacgg atggtcgagc
     4081 gggtgaccaa cgagctgggc cggctcgaca ttctggtcaa caacgctgcg ggaaattttt
     4141 acgcaccctc agccgacctg tcgcccaacg cgtggcgggc tgtagtggag accgatttgt
     4201 acggcacctt ctactgctgt caggctgctt acccggcatt ccgcgaggct ggagggggtc
     4261 ggatcatttc ggtatcgatg acgctgcact atcgcggctg gccgcagatg gcgcacgcta
     4321 ctgctgccaa ggcgggggtg gacgcgctca cccggacatt ggcgctcgag tgggcttccg
     4381 acaatatccg ggtcaatgca gtagcgcccg ggccaattcc cacggagggt gtgaagaaag
     4441 catttacgcc cccgggccgg gatgctccgg actttttcca gatggaagcc tacgccaaac
     4501 gatccattcc tgccggccga tggggagcgc ccgaggatat cgggcacatg gttacctatc
     4561 tcgccggccc cgccggtgac tggatcaccg gagcggtgtt cgtgatcgat ggcggacatt
     4621 ggctggtgcc gaccgaacgc cgataatgga acgggttttc tttgcgactg gcgccttgtc
     4681 gggcttcatt gccgttgccg caggcgcgtt cggcgcccac ggccttcggc aacaccttgc
     4741 gccagagcag ctttcggtct tcgagactgc ggcgcgctat cagatgtacc atgcgctcgg
     4801 attgctcgcg gtcgggtggg ccgcggcgcg ctggcctgga tcgccggcaa cctgggcggg
     4861 ttggctcttt atcgtcggca cgctgatctt ttcaggcagt ctgtacacat tggccctgac
     4921 cggccttggt tggttgggag caatcacacc aatcggcggg ctgtgtttcc tgggaggttg
     4981 gctctgtct
//
LOCUS       DASRJQ010000613         3199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_384501, whole genome shotgun sequence.
ACCESSION   DASRJQ010000613 DASRJQ010000000
VERSION     DASRJQ010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384501"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(329..1885)
                     /locus_tag="VFB89_13900"
     CDS             complement(329..1885)
                     /locus_tag="VFB89_13900"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF002868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0071897 - DNA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HZM28436.1"
                     /translation="MKFAALVQTSRRVSKANGRLEKIGLMADLLASVPPDEVEIATAY
                     LCGSVRQARLGLGWASLRNAMADAAAESPGLELVEVDTAFEQIALAAGKGSNQDKQRL
                     LRELLARATRAEQDFLGGLVMGELRQGALEGLVADAVARAASVPAELVRRAQMMVGDL
                     PKVAATALTEGAVGLSRFSIQVFRPVLPMLADTAEDEVEALSQLGEAALEFKVDGARI
                     QIHKSGDDVRIFSRRLNDVTEAVPELVEATRRLPARELIIDGEAAALRPDGAPHPFQA
                     TMRRFGRKLEIQRLLGELPLTPFWFDLLYLDGSAMLDEPLEHRFAALSSTVPAALVIP
                     RLVTSSPDQARSFLERALTSGHEGVMAKAPDAPYEAGRRGSRWLKIKPVQTLDLVVLA
                     AEWGHGRRHGWLSNLHLGARDAVSGGFVMLGKTFKGMTDQMLAWQTTRLLELEVARDG
                     HTVHVRPELVVEVAFNDLQASPLYPGGLALRFARIKRYRPDKAAADADTLQTLEAIYR
                     RQTGLEPRRP"
     gene            complement(1899..2510)
                     /locus_tag="VFB89_13905"
     CDS             complement(1899..2510)
                     /locus_tag="VFB89_13905"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HZM28437.1"
                     /translation="MKTQIVIFDGFDELDAVAPYEVLTAAGRRGGPFAVELAHLDGPR
                     EVEGSQGLRVQATAKLADRLELLVVPGGRWADRSSTIGARVEAERGLLPRAIARLHGN
                     GATIASVCTGAMLLSAAGLTQGRRVTTHHVAREALKASGAELVDARVVDDGELITAGG
                     ITSGLDLGLWLVERFGDPALALDLERQLEYERRGTVWQRGRGK"
     gene            2940..>3199
                     /locus_tag="VFB89_13910"
     CDS             2940..>3199
                     /locus_tag="VFB89_13910"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HZM28438.1"
                     /translation="MPEDSPGNRGTVSGVASTTYFVRLALLAGLYYLAARLGLRFASI
                     GESISLVWPPTGVAFAALTLLGFRYWPGVALGAFLANAATSVP"
BASE COUNT          528 a         1104 c         1004 g          563 t
ORIGIN      
        1 tgctaaagga ggcggacagg aattttccag cgtacgaaat cttcgcagat gcgggcgaag
       61 atggcgcctc gggggattcc ctctggctga tggatctttc tttcttacga ccccctcaag
      121 cccagggcag cttttcgagc gtttccccta caaccaaacg caaagcaggc tagctcggtg
      181 ttgctggtcg tcgcgacgat gatgacacag tatgccgcgc accgccttct cccagacgac
      241 tagtggctcc gaaacgctgt gcctagttag gtgcgctccg tcacggacaa agagcccgat
      301 ctcgtgcggc atcgcgagcc tcgccggact aaggccggcg aggctcgagt ccggtctgac
      361 gccggtagat cgcttcgagc gtttgcagcg tatccgcgtc ggccgcggcc ttgtcggggc
      421 ggtatcgctt gatccgggcg aatcggagag ccaggccccc aggatacaac gggctcgctt
      481 gcaggtcgtt gaaggcgact tccactacca gttcgggtcg gacgtggacc gtgtggccgt
      541 cccgtgctac ctcgagctcg aggagtctgg tggtctgcca cgcgagcatc tggtcggtca
      601 ttcccttgaa ggtttttccc agcattacga atccgccact cactgcatcg cgcgcgccca
      661 ggtgcaggtt gctgagccag ccatgacgcc tgccgtgccc ccactccgcg gcgagcacga
      721 ccaggtccag ggtctggacc ggcttgatct tgagccagcg acttccccgt cgaccggcct
      781 catagggagc atccggtgcc ttggccatga cgccttcgtg gccgctggtc aaggcgcgtt
      841 cgaggaatga tcgggcctga tccggcgagg aggttaccag cctcggaatc accagcgccg
      901 ccggtacagt gctcgatagc gctgcaaacc ggtgctcgag tggctcgtcc agcatggcac
      961 tgccgtccag atagaggagg tcgaaccaga aaggcgtgag cggaagttcg cccagcaggc
     1021 gttggatctc gagcttgcgg ccgaagcgac gcatcgtagc ctggaacgga tggggcgctc
     1081 cgtcagggcg aagcgcggcg gcctcgccat cgatgatcag ctcgcgcgcc ggcagcctgc
     1141 gggtcgcttc gaccagctcc ggaaccgcct cggtcacgtc gttgagccgg cgcgagaaaa
     1201 tccgcacatc gtcaccactc ttgtggatct ggatgcgggc gccgtcgacc ttgaactcga
     1261 gagccgcctc gcccaactgg gagagggcct cgacctcgtc ttcagccgtg tcggccagca
     1321 tcggaagcac cggccgaaag acctgaatcg agaaccggga aagcccgacg gcgccctccg
     1381 tcagtgcagt cgccgccacc ttgggcagat cgccaaccat catctgggcg cgccgcacca
     1441 gctccgccgg taccgaggca gcccgggcaa ccgcatccgc caccaggccc tcgagcgcgc
     1501 cctgccgtag ctcgcccatc actaggccac caagaaaatc ctgttctgcg cgggtggcgc
     1561 gggcgagcag ctcgcggagc agacgttgct tgtcctggtt ggaccccttg ccggccgcaa
     1621 gcgcgatttg ctcgaacgca gtgtcgacct cgaccagctc gaggcccggc gactcggcag
     1681 cggcgtctgc cattgcattt cggagcgacg cccagccgag gcccaatcga gcctgtcgca
     1741 cggatccgca gagatacgcg gtcgcaatct caacctcgtc gggcggcacg ctcgccagca
     1801 gatctgccat cagtccgatt ttctccagcc gcccattggc cttgctcact cgccgggacg
     1861 tctggaccag cgcggcaaac ttcatcggcc ggttccgctc atttccctct cccacgctgc
     1921 catacagtgc ctcggcgctc gtactccaac tgtcgctcca gatcgagcgc aagcgccggg
     1981 tcgccgaagc gctccaccag ccagaggcca agatccaggc ccgaggtgat tccgcccgcg
     2041 gtgatcagct ctccgtcatc gacgactcgc gcgtcgacca actccgcacc actcgctttc
     2101 agtgcttccc gggcgacatg gtgcgtggtc acccgccgac cctgcgtgag tccggcggcg
     2161 gagaggagca tcgcgccggt gcacacggag gcgatagtcg cgccgttgcc gtggagacgg
     2221 gcaattgcgc gcggtagaag cccgcgctct gcttctaccc tcgcgccaat ggtcgagctg
     2281 cggtcggccc agcgtccgcc cggcactacc agcagctcca gccgatcggc gagctttgcg
     2341 gtggcctgaa ctctgagacc ttgtgatccc tccacctctc gcggcccgtc caggtgagcc
     2401 agttccacag cgaacggtcc cccgcgccgg cctgcggccg tgagcacctc gtagggcgcc
     2461 acggcatcga gctcgtcgaa tccatcgaag atgacaatct gggtcttcac gcggctcact
     2521 ccgtttcgag tgcgagggga ccaggtgacc gccagccgag ccagtcaccg accgagctga
     2581 ctcggtgccg gacgatatct gctataagcc gcccgcgcag cacgcgagcc gctgacatgt
     2641 agtgcccgag gggcgtcaaa tcgcgttctt gtggctgccg gcctgatccg agagcgagtc
     2701 ggaggccgcg ggggaggctg gggttggcgg ggcatggcca gcctcccccg tggcctccca
     2761 ctcgctctcg gattagccgg tcccagaaaa cgcaactttt gtcgccccga gggggtctcc
     2821 atctcaacgg gagcgtgcaa cgtgcacggt ccactgcgac gcgggccgcc gcgagagtgc
     2881 gctggttgac acgacgccgg tgcaggcccg aagttgcagc ccaccaacgc acccgactca
     2941 tgcctgaaga ctctcccggc aatcggggta cggtctcggg tgtcgcctcg acgacctact
     3001 tcgtccggct ggcgctcctg gccgggctct actacctggc agcacggctg ggcctccgat
     3061 tcgcctccat cggcgagagc atctctcttg tctggccgcc aaccggtgtc gcgtttgctg
     3121 ccctgacact cctcgggttt cgttattggc ccggcgtcgc gctcggagca tttctcgcga
     3181 acgcggccac gtcggttcc
//
LOCUS       DASRJQ010000614         2124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2068949, whole genome shotgun sequence.
ACCESSION   DASRJQ010000614 DASRJQ010000000
VERSION     DASRJQ010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2068949"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VFB89_13915"
     CDS             complement(<1..352)
                     /locus_tag="VFB89_13915"
                     /inference="COORDINATES: protein motif:HMM:NF015441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HZM28439.1"
                     /translation="MPHLASLHIYPIKSCGGFSPPQWEVDGFGLRYDRRWMVVDLAGD
                     FMTQREEPRLALVRPALLDHSLALRAPGMPEIAIPLEPRERGRVPLRVWDDQTEGVPV
                     SPEAAQWLSRFLGKL"
     gene            complement(398..1288)
                     /locus_tag="VFB89_13920"
     CDS             complement(398..1288)
                     /locus_tag="VFB89_13920"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HZM28440.1"
                     /translation="MSRKTKETPVFGRKPSDADIDVYGVTHAGKVRPNNQDHFLIGSL
                     RKQMEIQLTSLPTIDTLVGRRERLAFIAMVADGVGGGPKGEEASRLAVERVTQYVSRT
                     MRCYYTADPTDEVAFSRSLMEAAMLSHMDIAEEVSRDPSRRGMATTLTLWISTWPRAY
                     LLQVGDSRCYLLRQGELRQISRDQTMAQDLVDQGIMTRTEAYQTRWANVLASAIGGSE
                     AAPVVTRLDPMWNDVGLLCSDGLTKHVPDARIRERLLSSSSAKQACEALLEDALDAGG
                     TDNITIVVGRTPSDPSAIGL"
     gene            complement(1387..>2124)
                     /locus_tag="VFB89_13925"
     CDS             complement(1387..>2124)
                     /locus_tag="VFB89_13925"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28441.1"
                     /translation="RTEPETLRTLYREAIRWGVRRLCLADTTGHATPPGVERLVRFVR
                     DEVISPSGEKIALDWHGHRDRGMAVANCFAAIAAGVDRVHGTALGCGERAGNAEMELL
                     LANLHLAGLPHGDLARLPEYCTLAARALRLRIPANHPVVGYDAFRTASGIHAAAILQA
                     HARGDVELAELTYSSLAASLFGLKQSFALSPMSGHAVVRHWLEQHGHDPADDQLVDSL
                     LAAAKQGDRVLEEADAERVVRGVGVAH"
BASE COUNT          377 a          688 c          677 g          382 t
ORIGIN      
        1 cgagcttccc gagaaagcga ctgagccatt gggccgcttc gggactcacc ggcacaccct
       61 ccgtctgatc gtcccacacc cggagcggca cccgcccacg ctcacgaggc tcgagcggaa
      121 tcgcgatctc cggcatgccg ggcgcccgaa gcgccaggga gtgatccagc aaggcgggtc
      181 gtaccagcgc gagccgcggc tcttctcgct gggtcatgaa atcgccggcc aggtccacca
      241 ccatccagcg gcggtcgtag cgcagcccga agccgtcaac ctcccactgt ggcggagaga
      301 agccgccgca ggatttgatg gggtagatgt gtagagaggc taggtggggc atggggttga
      361 agctatcagc tatcagctat cagctatcgg ctatcagcta gaggccgatg gctgatgggt
      421 ccgacggcgt ccggcccacc acgatcgtga tgttgtcggt gccgccagcg tcgagcgcat
      481 cttcgagcag agcctcgcat gcctgcttcg ctgaagagga gctgaggaga cgctcgcgaa
      541 tcctggcatc gggaacgtgc ttggtgaggc cgtcactgca gagcagtccg acgtcgttcc
      601 acatgggatc aaggcgggtc actactggtg ccgcttccga acctccaatt gcactggcga
      661 gcacgttcgc ccaccgggtc tgataggctt cggtgcgcgt catgatgccc tggtccacca
      721 ggtcctgagc catagtctga tcccgcgaga tttgacgcag ctctccctgc cgcagcaggt
      781 agcagcggct gtctcccacc tggagcaggt aggcccgcgg ccaggtgctg atccagagcg
      841 tcaatgtagt cgccatgcct cgacggctcg gatcgcggga tacctcttcc gcgatatcca
      901 tgtgagacag catcgccgct tccatgaggg agcgcgaaaa ggcgacctca tccgtcgggt
      961 ccgccgtgta gtagcaccgc atggtacggg acacgtactg cgtcacccgc tcgaccgcga
     1021 gtcgactggc ttcttctcct ttgggtccgc cgcctacacc gtctgctacc atcgcgataa
     1081 aggccagccg ttcgcgccga cctacaagag tgtcgatagt cggcaggctg gtaagctgga
     1141 tctccatttg tttccggaga gagccgatca gaaagtgatc ctggttgttc gggcggacct
     1201 tcccggcgtg ggtcacgcca tacacgtcga tatcggcatc gctcggcttt cggccgaaga
     1261 ccggcgtttc cttggttttc cgcgacacgg gcataaagga tcctgagctg ttagccgtta
     1321 gccggtggcc ggtggccgtc ggctaaaggc taatggttaa cgctaatggc tacagcccga
     1381 cagcctctaa tgcgcgacac caacaccccg caccaccctc tcggcgtccg cttcttccag
     1441 tactcggtcg ccctgcttgg cggccgcaag tagtgaatcg accagttgat catccgcggg
     1501 atcatgcccg tgttgctcca gccaatggcg aacgaccgcg tgacccgaca tcggcgagag
     1561 ggcaaagctc tgcttcaggc cgaagagtga cgcggccaga ctggagtagg taagctccgc
     1621 cagctcgaca tctccccgtg cgtgcgcctg aaggatcgcc gcggcgtgaa tgccgctcgc
     1681 cgtgcggaag gcgtcgtaac ccaccaccgg atggtttgcg gggatccgga gccgcagggc
     1741 tcgcgccgca agcgtgcagt actcgggcag gcgggcaaga tcgccgtgcg gcaggccggc
     1801 cagatggaga ttggcgagca gcagctccat ctcggcgtta ccggcgcgct cgccgcagcc
     1861 gagcgcggtg ccgtgtaccc gatccacgcc tgcggcaatc gctgcgaagc agttggccac
     1921 ggccatccct cggtcgcggt ggccgtgcca gtcgagcgca atcttctcac cggacgggct
     1981 gatcacttcg tcgcgcacga agcgaaccag tcgttccact cccggggggg tcgcgtgacc
     2041 ggtggtgtct gccaggcaca ggcgacggac tccccaccgg atcgcctccc gataaagcgt
     2101 tcggagcgtt tcaggctcag tgcg
//
LOCUS       DASRJQ010000615         4261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1270639, whole genome shotgun sequence.
ACCESSION   DASRJQ010000615 DASRJQ010000000
VERSION     DASRJQ010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270639"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VFB89_13930"
     CDS             complement(<1..254)
                     /locus_tag="VFB89_13930"
                     /inference="COORDINATES: protein motif:HMM:NF016213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 family protein"
                     /protein_id="HZM28442.1"
                     /translation="MSDPAGLVLLAAACVVGLLLIPLGLPGLWVIVLAVLGYGWLTDF
                     RTMSAGFVILITGLALAGELIEAWLGFRFAQRYGGSRRAG"
     gene            complement(251..712)
                     /locus_tag="VFB89_13935"
     CDS             complement(251..712)
                     /locus_tag="VFB89_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28443.1"
                     /translation="MHRHNWIFLALAFGVGSLGGCKKAPPPPPPSEVTTPPPEPPAPP
                     PFAVKTIDVGKGITADKHVANATTSFSVKDTIYVSVVTDGAAPSKTITARWTFGAGKL
                     VKEQSDTIAPTGPAATEFHITKKTPWPAGKYKVEILVDGNSAGTKEFEVKK"
     gene            complement(736..963)
                     /locus_tag="VFB89_13940"
     CDS             complement(736..963)
                     /locus_tag="VFB89_13940"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZM28444.1"
                     /translation="MLDNEPDFSDVEEGSSSTAPKPAGATYTVVKGDSLSKIAKRLYG
                     DAQQWKQIYEANKDTIKNPDQIYPGQVLKIP"
     gene            1128..2234
                     /locus_tag="VFB89_13945"
     CDS             1128..2234
                     /locus_tag="VFB89_13945"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZM28445.1"
                     /translation="MADATGPAPPKVALHVEPDELPPIETKETALRWLLLLGVLYTLY
                     FARELVVPIVVAVLLDFLLKPLVRGLRRIGVPEAAGGAIVVLGLLGSLGLAAYQLADP
                     ARSLVASAPETIQKATDRLRSVRSPLQKLERTAHQVEKAASQATDTTTREIAVKGPPL
                     SSKLLGGVRVFGAAALEVVILLYFLLASGDLFLQKLIKLLPQLRDKKTAVSIARETEG
                     AISTYLVTLTLINLGVALAVGGALALVGLPNPILWGALAGLAEFMPYVGATMIFVLLS
                     LAGLATFDHLGHALLVPGVYLAINLLQANLVSPLLHGRRLALNPVAIFLGLLLCWWMW
                     GVVGAFIAVPLLAAVRIFCDHIESLAPVGEFLGE"
     gene            2258..>4261
                     /locus_tag="VFB89_13950"
     CDS             2258..>4261
                     /locus_tag="VFB89_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28446.1"
                     /translation="MAGDPLAVVSFIAQVARRRTIAGDVSITSVTSPPFLARFRSRGT
                     GTPDEPLTGPIRGELLGAEHLADRAQAVAAAQKVVERRRPPRRPRLLVRLDETRQILD
                     DVHTRLAEAADRDVDVGPAGEWLLDNFHIVQEHIREVRQSLPRGYYRELPELAGGTLP
                     GYPRVYELAITLISHTEGRIDLGNVELFVDAFQKVSPLSIGELWAVPAMLRLGLLENV
                     RRMALRTIHRLDELELANQWAERLIAASGDTASVLSAAINDFVAHAPPLTPTFVSRFL
                     SQLRSVGVSVPSLTWLERWVSDEAMTAEDAAARATQRVALTQIMMANSITSLRAIARM
                     DWDSFVEHQSVMEVVLREDPVYPRMTFATRDRYRHVVERIAKRARTDEAAVARVALEL
                     ARRAPTDDVGPERSAHVGYYLLDQGRTAFEHAVGYRPTALERVHRWILRHPNFVFVGG
                     MLLGTALALAALLWLAGVSGEAAMIAVVLLALIPANDIAINVLNQLVTAFLPPHVLPK
                     LDLSEHGVPPELRTAVVIPTLFDSVAAVRDGLDHLEVQYLANREAHLHFAVLSDFTDS
                     PSETRPDDAAILEAAVAGVRELNARYAPETQDAFYLFHRPRRWNPRQGVWMGWERKRG
                     KLAEFNRFLRGGARKAFSAIVGNTAPLAHVRYVITLDSDTVLPP"
BASE COUNT          684 a         1421 c         1350 g          806 t
ORIGIN      
        1 caccccgccc tacgcgatcc gccgtagcgc tgcgcaaacc gaaatcccag ccaggcttcg
       61 atgagctcgc cggccagcgc cagcccggtg atcaggatga cgaagccggc gctcatcgtg
      121 cggaaatcgg tgagccaccc gtaacccagc accgcgagca cgatcaccca caggccgggc
      181 agtccgagtg gaatcagaag caggccgacg acgcacgccg cggcgaggag aacgagcccg
      241 gccgggtcac tcacttcttg acttcgaact ccttggttcc cgccgagttt ccgtcgacta
      301 ggatttcgac cttgtacttg ccggccggcc aaggcgtctt cttggtgatg tggaactcgg
      361 tggccgccgg tccggtcggc gcgatcgtgt cgctctgttc cttcaccagc tttccagccc
      421 cgaaggtcca gcgagccgtg atggtcttgc ttggcgcggc cccgtcggtc acgaccgaca
      481 catagatcgt atccttaacg ctgaagctcg tcgttgcgtt ggcaacgtgc ttgtccgcgg
      541 taatgccttt gcccacgtcg atcgtcttga ccgcaaatgg cggcggagcc ggcggctccg
      601 ggggaggcgt cgtgacctcg ctcggcggcg gtggcggcgg cgccttcttg cacccgccca
      661 gtgagcccac gccgaaagcc agggcgagga agatccagtt atgacgatgc atgcaattat
      721 ctccgagtct cgggcttacg ggatcttgag cacctggccg ggatagatct gatccggatt
      781 cttgatagtg tccttgtttg cttcgtagat ctgcttccac tgctgcgcgt cgccatagag
      841 ccgcttggcg atcttggaca gactgtcgcc tttcaccacc gtataggtcg cccccgccgg
      901 cttgggcgcc gtcgacgagc ttccctcctc cacgtcggag aagtcgggtt cgttgtccag
      961 catgaaaacc tccagtgagg ttgcaaaagc gattctaagg taccctccgg cgccggaggg
     1021 ggccattcaa tgggaagccg gcacctcacc tcgaaaagtg agtcgcatca gtgattgttg
     1081 tgacggataa ccccgcacgc aggggcccga acggctagtc tgtcccaatg gctgacgcca
     1141 ccggccctgc gccgccgaaa gtagctctgc atgtcgagcc ggacgagctg cctccgatcg
     1201 agaccaagga gacggcgctt cggtggctcc tgctcctggg tgtgctctac acgctctact
     1261 tcgcccgaga gctggtggtc ccgattgtcg tggccgttct gctcgatttc ctgctcaagc
     1321 cgctggttcg cgggttgcga aggatcggcg tacccgaagc agccggcggc gcgatagtcg
     1381 tcctcgggct gctgggatcc cttggtttag cggcgtatca gctggcggat ccggcacgga
     1441 gcctcgtggc cagcgctccc gaaacaattc agaaagccac cgaccgactt cgctcggtgc
     1501 ggagtccgct tcagaaactg gaacgcaccg cccatcaggt cgaaaaagcg gcgagccagg
     1561 cgaccgacac gacgacccgc gaaatagcgg tgaaaggtcc gccattgagc tccaagctgc
     1621 tgggtggcgt gcgggtgttc ggggccgccg cgctggaggt cgtcatcctt ctctacttcc
     1681 tcctagcctc gggtgacctg tttctccaga agctgatcaa gctgctgccc cagctgcgcg
     1741 acaagaaaac cgccgtctcc atcgcgcgcg agaccgaagg cgccatctcc acctatctcg
     1801 tgaccctcac gctcatcaat ctcggtgtgg ctctcgccgt cggcggcgcg ctggcactgg
     1861 tggggcttcc caacccgatc ctctggggag cgctcgcggg gctggcggag ttcatgccgt
     1921 acgtcggtgc cacgatgata ttcgtgctgc tctcgcttgc gggcctcgcg acgttcgatc
     1981 atctgggcca tgccctgctg gtccccgggg tgtatctcgc catcaacctt cttcaggcca
     2041 acctcgtcag cccgctgtta cacggccgtc gactcgcgtt gaatccggtc gccattttcc
     2101 tggggctcct gctctgctgg tggatgtggg gtgtggtggg tgcattcata gccgtgcctc
     2161 tgctggctgc agtccggatc ttctgcgacc atatcgagag ccttgcgccg gtaggagaat
     2221 tcctgggtga gtgaaaggcg ctgcccctgt gccatagttg gccggcgacc ccctcgccgt
     2281 agttagtttt atcgctcagg ttgcccgccg acgtaccatc gcaggagatg tctccattac
     2341 ctcggtaaca tcaccgccgt tcctggcccg cttccgctcc cgcggcaccg gcactcccga
     2401 tgagccgctc actggtccga tccgcggcga gctgctgggc gccgagcatc tcgcggaccg
     2461 ggcccaggcg gtcgcggccg ctcagaaggt tgttgagcga cgtcgcccgc cccgtcggcc
     2521 ccggctgctg gtgcggctcg atgagacccg ccagatcctg gatgacgtgc acaccaggct
     2581 ggctgaagcc gccgaccgtg atgtcgacgt aggacctgcc ggcgaatggc tgctggacaa
     2641 cttccacatt gtccaggagc acatccgcga agtgaggcag agtctgcctc gcggctacta
     2701 ccgcgagctc cccgagctgg cgggcggcac cctgccgggc tacccacgcg tctacgagct
     2761 ggcgatcacg ctcatctcgc acacggaagg gcggatcgac ctcggcaacg tcgaattgtt
     2821 cgtcgacgcc ttccagaaag tctctccgct ctcgatcggc gagctgtggg ccgtacccgc
     2881 gatgctgcgg ctcggcctcc tcgagaacgt gcggcgaatg gcactccgta cgattcaccg
     2941 gctcgacgag ctggagttgg ccaatcagtg ggcggagcgg ctcatcgcgg ccagcggcga
     3001 cacggcatcg gtgctctcgg cggcgatcaa cgatttcgtc gctcacgcgc cgccgctcac
     3061 tcctaccttc gtctcgcgtt tcctgagcca gctgcggtcg gtgggcgtct cggtgccgtc
     3121 attgacctgg ctcgagcgct gggtcagcga cgaagcgatg accgccgagg acgccgcggc
     3181 gcgcgccact cagcgggtgg cgctcaccca gatcatgatg gcgaacagca tcaccagctt
     3241 gcgggccatc gctcgcatgg actgggactc gttcgtcgag caccagagcg tgatggaggt
     3301 ggtgctgcgg gaagatccgg tttacccgcg gatgaccttc gctacccgcg accgttaccg
     3361 gcacgtggtg gaacggatcg ccaagcgcgc ccgtacggac gaagccgcgg tcgcccgagt
     3421 cgcactcgag cttgcccgcc gggctccgac tgacgatgtg ggtccggagc gatcggcgca
     3481 cgtgggctac tacctgctgg atcagggccg cacagcgttc gagcacgccg tcggttaccg
     3541 accaaccgct ctggaacggg tgcaccgctg gatcctgcgc cacccgaact tcgtgttcgt
     3601 gggcggaatg ttgctcggca ccgccctcgc cctggccgcc ctgctgtggc tggccggcgt
     3661 gtccggtgag gccgccatga ttgcagtcgt tttgctggcg ctcattccgg cgaacgacat
     3721 cgcgatcaac gtgctcaatc agctggtgac ggcatttctg ccgccgcatg tgctgcccaa
     3781 gctcgatctg agcgagcacg gggtcccgcc cgagctgcga acagcggtgg tcatccccac
     3841 gctgttcgac agcgtagccg cagtccgcga cggactcgat cacctcgagg tgcagtacct
     3901 ggccaaccgg gaagcgcatc tgcatttcgc cgttctgagc gacttcaccg attcgccgag
     3961 cgagacccgg cccgacgatg ccgcaatcct ggaagccgcg gtggccggcg ttcgcgagct
     4021 gaacgcccgc tacgcaccgg agacccagga cgccttctat ctcttccatc ggcccaggcg
     4081 ctggaaccca cgccagggcg tgtggatggg atgggagaga aagcggggga agctcgccga
     4141 gttcaatcgc tttctccgcg gcggggcacg gaaggcgttc tcggcgatcg taggcaatac
     4201 ggcgccgctg gcccacgtgc gctacgtgat cacgctcgat tccgacaccg tgctgccgcc
     4261 g
//
LOCUS       DASRJQ010000616         4803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1228554, whole genome shotgun sequence.
ACCESSION   DASRJQ010000616 DASRJQ010000000
VERSION     DASRJQ010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228554"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VFB89_13955"
     CDS             <1..193
                     /locus_tag="VFB89_13955"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28447.1"
                     /translation="GGRPAGSISAGWFLREFVDGFAWAHLDVAGTAYTEREEPGRVKG
                     PTGIGVRLFSEFVLNRSKA"
     gene            257..2164
                     /locus_tag="VFB89_13960"
     CDS             257..2164
                     /locus_tag="VFB89_13960"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HZM28448.1"
                     /translation="MRALVLLIVPFTVYHSPFTPPLSAQQPDTLHPLLPGQDTTHVVR
                     PDTARAPFDTTGYIRDTGYVADTTGRKARIDSSGVQRYLALEKLKETRVLVPAPIDVP
                     GPEPPLSRLVFNRDSIDWAHASSLADLLQRVPGVYLWRGGWTGQPEYANFQAQGATSV
                     EYWIDGIPYIAAGTDSVGVDPSIFSLSILDRIEIERWPGLLRVRMYTRNHDRLAPRSR
                     IAISRGAADFARYQGSLELKARSGLGLMLAGDNLTVPAVQNIRSRYANTVLLLQGTFL
                     PSPHWGLVLQGLRSRPDRDPTLAAGDTLSRGLPNARRMDWLLRAMWRADTSGFGPRAD
                     LVFNRTTASDSLTPNQTINQVGLIGSLRGPAYSLGASAFYRSRWTSADLRATAGWSPV
                     GWVALSGEGAYQRHDGGRSSNWAGLRAGVRLPLGLEASATLRAGKVVAAPAVLTDQAQ
                     TVRDWQLSAGWEQRWFALEGGLAHTSEYQPQAYQPYAQIPVISPSGATDWLTGRVRLT
                     PLSWMSFEGWVSNPRNVVPEGLPPRHMLGSGTIRTKLLRVFPSGTLDIELRLAVEHWS
                     EGVLGRDPLGNPVAVPSATYLRSLVQIALGSFQFYWDRSNLDTTENPYVPGLPIVGRP
                     SEFGVRWTFAN"
     gene            complement(2631..3086)
                     /locus_tag="VFB89_13965"
     CDS             complement(2631..3086)
                     /locus_tag="VFB89_13965"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HZM28449.1"
                     /translation="MYEPTSRRSASPFQSLQRLNTMLDEAFWPWSNRQGDYGTITAAW
                     APACDVFEDPESVRIIAEVPGVNPEDVKLSMEGNVLTIRGEKKQRAEEKTERVHRYER
                     SYGWFERAFSLPNTVDPDRIEAKVDQGVLTVVLPKAERARPREIPVKTS"
     gene            3467..4330
                     /locus_tag="VFB89_13970"
     CDS             3467..4330
                     /locus_tag="VFB89_13970"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HZM28450.1"
                     /translation="MTGFGAGEGPVAGGRLRIEIRTVNHRYFNLAARLPSELSSVEGD
                     LRERLRRDFDRGHLAIQGRWITQPARAEPIGLDLERAKAIVARLRELQSALALSGDVS
                     LELVARQPDVFGANDGQPVELAWAEVEPIVAAAAAECRAMRRREGEVLAAELCQRLDL
                     LDQGAQIITARAPERLIRERDRLRAGVRELLEGRSVDEQRLAQELAFMADKLDITEEL
                     VRFGAHVKAAREALASARPVGKQLGFLAQELGREINTIGAKANDAEIAHRVIGMKGEL
                     EKLREQIENLE"
     gene            4327..>4803
                     /gene="gmk"
                     /locus_tag="VFB89_13975"
     CDS             4327..>4803
                     /gene="gmk"
                     /locus_tag="VFB89_13975"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HZM28451.1"
                     /translation="MRPLLLVLSSPSGGGKTSIARQLLNARADVGYSVSATTRAPRAG
                     EEDGRDYHFLARPEFEHRLAAGEFVEHAAYGGQLYGTLRSEIDRIFGARKHAVLDIEV
                     EGARQIRQAMPGAVQVFVLPPSGRELIRRLEGRRTEDRLAMRRRLDRADEELAAVGE"
BASE COUNT          805 a         1536 c         1574 g          888 t
ORIGIN      
        1 cggcggccgg ccggctggta gcatctccgc cggctggttc ctccgcgagt tcgtcgatgg
       61 atttgcctgg gcccatctcg atgtcgcggg caccgcgtac accgagcgag aggagcccgg
      121 cagggtaaag ggtcctaccg gaatcggcgt gcggctgttc agcgagttcg tgctgaatcg
      181 aagtaaggca tgagcgcttg aacagcgtga acggtgaatg gtgaacggtg aacgggggcg
      241 agcgctaagg attgggttgc gcgcgcttgt cctcctgatc gtcccgttca cagtttatca
      301 ttcaccgttc acgcctccgc tgtccgctca acagcccgac accctccatc ccctgcttcc
      361 cggacaggac actacccacg tcgttcggcc ggacactgcc agagccccgt tcgacacgac
      421 gggctacatc cgcgacacgg ggtacgtagc cgataccacg ggcagaaagg cccgtatcga
      481 ttcgagtggg gtgcagcgtt atctcgccct ggagaagctc aaggaaacca gggtgcttgt
      541 cccggcgccg atcgacgtgc ccgggcccga gccgcccctg tcgcggctgg tgttcaaccg
      601 cgactcgatc gactgggctc acgcgtccag cctggcagac ctgctgcaac gagtccccgg
      661 cgtctacctc tggcgcggcg ggtggaccgg tcagcccgag tacgcaaact tccaggccca
      721 gggcgcgacg tcggtggaat actggattga cggcattcca tacatcgcgg cgggcaccga
      781 ctcggtaggc gtcgacccgt cgatcttctc gctcagcatt ctggaccgga tcgagatcga
      841 acgctggccg ggccttctgc gggttcggat gtataccagg aaccacgatc gcctcgcgcc
      901 gcgctcacgc atcgcaatct cgcggggagc cgcggacttc gcccgctatc agggctcgct
      961 cgagctcaag gcccgctcgg gtctcggcct catgctcgcg ggcgacaatc tgactgttcc
     1021 ggcggtccag aatatccgtt ccaggtacgc caatactgta ctgctccttc aggggacctt
     1081 tctcccgtcg ccccactggg gtctggtgtt acaagggctg cgctcgcggc cggatcgaga
     1141 ccccacgctg gctgccggag acacattgag tcgcgggctg ccgaacgcgc gtcgcatgga
     1201 ctggctcctt cgggccatgt ggcgcgccga caccagcgga ttcggtcctc gggcggatct
     1261 ggtcttcaac cgaaccaccg cgagcgacag cctcacgccc aaccagacca tcaaccaggt
     1321 gggactgatc ggctcactcc gcggaccagc gtactcgctc ggtgcctcgg ccttttatcg
     1381 ctcgcgctgg acctcagccg accttcgtgc cacggccgga tggtcgccgg ttggttgggt
     1441 ggcgctgagc ggggaagggg cctaccagcg ccacgacggc ggccgcagct cgaactgggc
     1501 cgggcttcgc gcgggtgtac gcctgcccct ggggctcgaa gccagcgcga ctctgcgcgc
     1561 cggtaaagtc gtcgctgcgc cggcggtcct cactgatcag gcgcaaaccg tgcgcgactg
     1621 gcagctgtcc gccggctggg aacaacgctg gttcgcgctc gagggtggat tggctcacac
     1681 ctctgagtac cagccgcagg cataccaacc gtacgcgcag attccggtca tcagtccttc
     1741 cggcgcgacc gactggctca ccggtcgggt gcggctgaca ccgctctcgt ggatgtcgtt
     1801 cgagggttgg gtgagcaacc cgcgcaatgt cgtacccgag ggcctgccgc ctcgccacat
     1861 gctcggcagc ggaaccatcc gcaccaagct gctgcgggtc tttcccagcg gaaccctcga
     1921 catcgagctt cgcctcgcgg tggagcattg gagcgagggg gtgctgggcc gcgacccctt
     1981 gggcaacccc gtcgcggttc cgagcgcgac ctatctgcgc agcctggtcc agatcgctct
     2041 cggatcgttc cagttttact gggatcgctc gaatctcgac acgacagaga atccctacgt
     2101 accaggattg ccgatcgtag ggcggccaag tgagtttggc gtgcgctgga cgtttgccaa
     2161 ctgagccgtg ccatgatcag cttagctccg tctcagttga taacccgaag tgcttaccac
     2221 taaatggatt gtgagttctc cctgacaaac tgccgtaatg ccacccaaac ggctttgcac
     2281 gctcttgttc gcgatgtgcg ttgagactcg gttgcgatcg aggcacgctg ctgcccgcag
     2341 gttgttagtg gcccagttta gggccaccac ccgcaggtgg tagcgggtca gtttgattgt
     2401 tacgacggcg gacggcggct tggtgacgag cgttaccaaa tcgagccggt ctgattgctc
     2461 ggccgtgcac aatacgggtg cttcgtctgc actcgatctc ttcaatcggc cgagtcggac
     2521 ttagtcgggg gtcaggccga gctggtgaag aagggcctcc ccccgaaacc gggaggaggc
     2581 ccggaaagtg gaggactggc tcctaggagc gttccaccat caattctcca tcagctcgtc
     2641 ttcaccggaa tttctcgggg tcgcgcacgc tcggcctttg gtagcaccac cgtgagcaca
     2701 ccttggtcaa ccttggcctc gatccgatca ggatcgaccg tattgggcag tgagaaggct
     2761 cgctcgaacc agccgtagct ccgctcgtag cgatggaccc gctcggtctt ctcctctgct
     2821 cgctgcttct tttccccgcg aatggtgaga acattgccct ccatggagag ctttacatcc
     2881 tccgggttca caccgggaac ctcagcaatg atcctgaccg actcggggtc ctcgaacacg
     2941 tcgcaggctg gagcccaagc ggccgtaatg gtcccataat ctccctgcct gtttgaccag
     3001 ggccagaacg cctcgtcgag catggtattc agccgctgga ggctctggaa cgggctagcg
     3061 ctccgccgag aggttggctc atacattttt ccatcctcca taaagtgtgg tttgatagtc
     3121 tgggggccgg cgtgttgccg attcgcgatc cggtcggagt gcagcggttg tgccaagtct
     3181 tggcccattg tgtatgtcaa agtgaccgcg aagagcagga ggtgatctgc caagcagaac
     3241 gatggtctgc aaaggtgacc gggttgtggg ccgtctgcgg taagcgtagt gcgtcagttt
     3301 gacgtaacgg gccgggcccg gtcccatcag agccaggatg tcctcgacct tccaaacgta
     3361 ggtggtcagg cccgctgccc cgcaggttcg cgacctaccc gtaacctccg ttaaccgcta
     3421 catttcccgg ctttcccgca ctcgggagtt caccctgccg ctcagcatga cggggttcgg
     3481 tgccggggag ggacccgttg ccggcggacg actgcggatc gagatccgaa cggtcaacca
     3541 ccggtacttc aacctcgcag ctcgactccc ctccgagttg tcgagcgtcg aaggtgactt
     3601 gcgggagcga ctccggcgcg atttcgaccg cggccatctt gcgatccagg gtcgttggat
     3661 cactcagcct gcgcgcgccg aaccgatcgg actcgacctc gagcgggcca aggcgatcgt
     3721 agctcggctc cgcgagctgc agagcgcctt ggctctgtcg ggagacgtgt cgctcgagct
     3781 ggtcgcccgg cagcccgatg tgttcggcgc caacgacggt cagccagtcg agctggcctg
     3841 ggccgaggtc gagccgattg tcgcagcggc cgcggcagag tgtcgggcca tgcgccgccg
     3901 cgagggcgaa gtgctcgcgg ctgagttatg tcagcggctc gacttgctgg accagggcgc
     3961 ccagatcatt acggcacggg cgccggagcg attgatccgg gagcgcgatc gactgcgggc
     4021 cggtgtccgc gagctactgg aagggcgatc ggtagacgag cagcggctgg cgcaggagct
     4081 ggcgttcatg gccgacaagc tcgacatcac cgaagagctg gtgcggttcg gcgctcatgt
     4141 aaaagccgcg cgcgaggccc tggcctctgc ccggccggta ggcaagcagc tcggcttcct
     4201 ggcgcaggag ctcggtcggg agatcaacac gatcggcgcc aaggccaacg atgccgagat
     4261 agcccaccgc gtgatcggca tgaaaggcga gctggagaag cttcgcgagc aaatcgagaa
     4321 tctcgagtga ggccgctgct gctcgtgctc tcgtcgccgt cgggtggcgg caagaccagc
     4381 atcgcccggc agctcctcaa cgctcgcgca gatgtgggat actcggtgtc ggccacgact
     4441 cgggcgcctc gagcgggtga agaggatggg cgggactatc acttcctggc ccgccccgag
     4501 ttcgagcacc ggctcgccgc gggcgaattc gtggagcacg cggcgtacgg cggtcagctg
     4561 tacgggacgc tgcggtccga gatcgaccgg atcttcgggg ccaggaagca cgcggtgctc
     4621 gacatcgaag tcgaaggcgc tcgccagatc cgacaggcga tgcccggtgc ggtccaggtg
     4681 ttcgtcctgc ccccttccgg gcgggagttg attcgccggc tcgaagggcg gcgcaccgaa
     4741 gatcgcctcg cgatgcggcg ccggctcgat cgggccgacg aggagctggc cgccgtaggc
     4801 gag
//
LOCUS       DASRJQ010000617         5309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3767353, whole genome shotgun sequence.
ACCESSION   DASRJQ010000617 DASRJQ010000000
VERSION     DASRJQ010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3767353"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="VFB89_13980"
     CDS             complement(<1..900)
                     /locus_tag="VFB89_13980"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HZM28452.1"
                     /translation="MSHEREQRVVFLSAVRTGFGAFGGTLRSHSATDLGAIATQCALA
                     RSGVTSDEIDHVIFGNVVQTSTDAAYLARHIGLSAGLPIEVPAVTVNRLCGSGFEAII
                     QGAHRILLGEAKAVVAGGTESMTQAPYVLRGARFGTKLGQAPVLEDSLWDSLRDTYCG
                     LPMGDTAENLGKQYGVTRADADAYALSSQQRAKSAWEAGAFRQEVIPVAVRNPKTGTT
                     TEWVADEHMRPDSTAEGLAKLPPVFRKDGLVTAGNASGVSDGAAAVVLADERWAAERG
                     LRPLGRLIAWAVSGVDPALMGIGP"
     gene            complement(991..1671)
                     /locus_tag="VFB89_13985"
     CDS             complement(991..1671)
                     /locus_tag="VFB89_13985"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZM28453.1"
                     /translation="MSDLERFFRQLVANIVATDPHRLHQQLQVAEILRDILPYRVNRR
                     ALGLESAEDYEVLLLRLCSGEGGYARIEPPELQARLQTELAGTNPDLGLLRANGDALV
                     WLGAAEVARALAAEPAVSYAPPGPPPQTVREETLRGTFDHTETQVAEPNNELPLDTVV
                     PPPPRSDADATAMCLYCGGALPAGRQVNFCPHCGQSQRPLQCPSCHADVEYGWRHCVS
                     CGATIGED"
     gene            1740..1994
                     /locus_tag="VFB89_13990"
     CDS             1740..1994
                     /locus_tag="VFB89_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28454.1"
                     /translation="MCIPPDNSNEAKVLGNFAVPLAFGPLAAPSEGTPDWVQLMLEGK
                     YIPTIDSATRMATICRPGRSPENTGLLAAFPRPRVAIGLP"
     gene            2053..2190
                     /locus_tag="VFB89_13995"
     CDS             2053..2190
                     /locus_tag="VFB89_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28455.1"
                     /translation="MWEGGVSRDAGSDFGLAGEIYPAPADTVTGRVGVTYSWRWWLES
                     A"
     gene            complement(2193..2750)
                     /gene="msrB"
                     /locus_tag="VFB89_14000"
     CDS             complement(2193..2750)
                     /gene="msrB"
                     /locus_tag="VFB89_14000"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HZM28456.1"
                     /translation="MGLVVALLLAVVGVAGAQTGTAPVATKGWNAVNYRKPTDAELKQ
                     RLTSTQYEVTQKEGTEAPFHNAYWDNHEAGIYVDVVSGEPLFSSLDKYDSGTGWPSFT
                     RPLEPKNLETKVDRSLMMVRTEVRSVHADSHLGHLFDDGPAPTGQRYCMNSAAMRFIP
                     VDQLEAQGYGQYLHLFQKSGAKGSK"
     gene            complement(3181..4014)
                     /locus_tag="VFB89_14005"
     CDS             complement(3181..4014)
                     /locus_tag="VFB89_14005"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HZM28457.1"
                     /translation="MKRREFVSAVGTIALAGSAGVFRRTKDRLDRIGLELYTVRDELA
                     RDFEGTLQRIAGLGYREVEFTDYFGRSVNAVRAAVATAGLTAPSAQVPSSDLRTAYAS
                     RIDRAKAIGHTYLVCGWVPPAERRSLSDWHSLIALFKTVGQAAHRAGLQFAYHNHDFE
                     FARVEGHVPYDLLLEATDPSLVQLELDVYWATKGGRNPVDLLARWPGRFPLLHLKDMA
                     SGPQHEFTELGRGIIDFRQILAQARTGGVRHLYVEQDEGRVPPFERARIGYDYLRALR
                     F"
     gene            complement(4017..4649)
                     /locus_tag="VFB89_14010"
     CDS             complement(4017..4649)
                     /locus_tag="VFB89_14010"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HZM28458.1"
                     /translation="MPRPSPIAVLHLDESCLGNGREGTTPGGAGGLIEVRTRSGIQRR
                     DFFLAAPDTTNNRMALSGAIAALQLLAAKGVRMRALMVSDSEYLVKGIREWAPGWAAR
                     GWKRKGGEIENLELWQALVRSSALHDVQWTWVRGHRGHPKNEYANDLAVEAAERQETS
                     AGAIDSGFGRWLATKQSRGLFSGYDPDAAFAELERRLAAGEVLPVAESRA"
     gene            complement(4649..4873)
                     /locus_tag="VFB89_14015"
     CDS             complement(4649..4873)
                     /locus_tag="VFB89_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28459.1"
                     /translation="MRWTADRLDQLERAVRDGLRVALTTRKGTEYVVVAARLATDGPR
                     EALIARLPMTGEELSFVLDDIASFQVLKGT"
     gene            complement(4897..>5309)
                     /locus_tag="VFB89_14020"
     CDS             complement(4897..>5309)
                     /locus_tag="VFB89_14020"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HZM28460.1"
                     /translation="SAATDVTGFGLVGHLLNLLEASRVGGELYFDAIPIFPHARNLAA
                     RGFVPGGTMRNLEAANERVSWAAELSETDRLLAVDAQTSGGLLLAVPPENERALLEAL
                     QTRGTPVAVTIGRLTADHPGRLEVLANPPGGVAS"
BASE COUNT          932 a         1728 c         1684 g          965 t
ORIGIN      
        1 cggaccgatg cccatgaggg cgggatccac gccgctgacg gcccaggcga tcaagcgacc
       61 cagtggtcga agtcctctct cggccgccca tcgctcgtcg gccaggacga ctgccgccgc
      121 tccatcggag acgcccgaag catttcccgc cgttacgagg ccgtccttcc ggaacacggg
      181 cggaagcttt gccagcccct cggcggtcga gtcgggacgc atgtgctcat ccgccaccca
      241 ttcagtggtg gtgccggtct tgggattccg taccgcgacc gggatcacct cctggcgaaa
      301 cgcgcccgcc tcccaggcag acttggcgcg ttgctgactg ctcaaagcat aggcgtctgc
      361 gtcggcgcgc gtgacaccgt attgctttcc caggttttcg gcggtgtcgc ccatgggaag
      421 gccgcagtac gtgtcacgca gcgaatccca caggctgtct tctaaaacgg gcgcctgacc
      481 gagcttcgtt ccgaatcggg cgccgcgcag gacatacggc gcctgggtca tggactcggt
      541 gcctcccgca acgaccgcct tcgcttcccc caacaggatg cgatgcgcgc cctggattat
      601 cgcttcgaac cctgacccac agagccggtt gacggtgact gcggggactt cgattggtaa
      661 gccggcgcta aggccgatgt gccgcgccag ataggcagcg tcggtggacg tctggaccac
      721 gttgccgaag atcacatgat cgatttcgtc ggaagtgact cccgagcggg ccaacgcgca
      781 ctgcgtcgcg atcgcgccga ggtccgttgc cgaatggctc cggagcgtgc cgccgaaggc
      841 cccgaatccg gtgcgcacgg cagaaaggaa gactacgcgc tgctcgcgct cgtggctcac
      901 gggatgctcc agcgaggggc ccaacaggag ggacgggcga acgacatggg actcggaata
      961 tggccccacc cgagaggcgc ttcaagcgag ctagtcctcg ccgatggttg cgccgcaact
     1021 cacgcagtga cgccagccgt attcgacatc tgcgtgacag gaggggcact gcagcgggcg
     1081 ctggctctgg ccgcaatgag gacaaaaatt cacctgccgt ccggctggta gcgcaccgcc
     1141 acagtagagg cacatggctg tcgcgtccgc gtcggaccgt ggcgggggcg gtacaacggt
     1201 gtcgagcggc agttcgttgt tgggttctgc gacctgggtt tccgtgtggt cgaaggtccc
     1261 gcgaagcgtc tcctcccgga ccgtttgggg aggtggccca ggcggcgcgt acgaaacagc
     1321 cggttccgcg gcaagggcgc gggcgacttc agcagcgccg agccagacaa gtgcgtcgcc
     1381 attcgcacgc aagaggccca ggtccggatt ggtgccggca agctcggtct gaagtcgcgc
     1441 ttgcagttcg ggcggctcta ttcgagcata cccgccttcg cccgagcaga gtctgaggag
     1501 aaggacctcg taatcctcgg cgctttccag tccgagtgcg cgccggttga cccggtacgg
     1561 cagaatgtca cgcaggattt cggcgacctg gagctgctga tgcagccgat gcggatcggt
     1621 ggccacgata ttggcaacca gctggcgaaa gaagcgctcg agatcgctca caggcttgca
     1681 ggtttagtgt tggggcaggt tacgggggga tgtccggaag tgtcaagcaa ggacacctga
     1741 tgtgcattcc acccgacaat tcgaacgaag ccaaggtcct cggaaacttc gcagtgccgc
     1801 ttgcattcgg acctttggcc gctccgtccg agggaacacc ggattgggtc cagctcatgc
     1861 tcgaaggcaa gtacattccg acgatcgact cggctacgcg catggccaca atctgcaggc
     1921 ccggtaggag tccggagaac accggccttc tggctgcttt cccgcgaccc cgcgttgcta
     1981 tcggacttcc ataggcatta ctgctcgagg gtagctgggt cccgccgatc acgctgaatg
     2041 gggtgacccc gaatgtggga aggaggcgtc tcgcgggatg cgggcagcga tttcggcctg
     2101 gccggtgaga tctaccctgc ccctgcggac accgtcaccg gccgggtagg cgttacctac
     2161 agctggcgct ggtggctcga gtcggcttga tcttacttgc tacccttggc gccgctcttc
     2221 tgaaacaggt gaagatactg gccgtatccc tgggcctcca gctgatccac cgggatgaag
     2281 cgcatcgctg cagagttcat gcaatagcgc tgtccagtcg gggccgggcc atcgtcgaac
     2341 aggtgcccca ggtgtgagtc ggcgtgaacc gagcgcacct cggttcgcac catcattaag
     2401 ctcctgtcca ctttcgtctc gagatttttc ggctcgagcg gcctggtgaa gctcggccag
     2461 cccgtaccag agtcgtactt gtcgagcgaa ctgaacaagg gctcccccga aacgacatcc
     2521 acgtagatcc ctgcctcgtg gttgtcccag taggcgttat ggaagggagc ctcggttccc
     2581 tccttctggg tcacttcgta ctgggtcgac gtgagccgct gcttcagctc cgcatcggtt
     2641 ggctttcgat agttcaccgc attccaccct ttcgtggcca cgggggccgt cccggtctgc
     2701 gctcctgcca cgcctacgac cgcgagcagg agtgcaacca caagtcccat cgcctgtctc
     2761 atttagcttc tccggatatc atcacccaac ttatacggtt ggtatcaccg cccaaagaac
     2821 gccgcttcgg caagcttggt ttcccggccg gaccggccag ccggtcatga gcaggtcgac
     2881 atgcccgcag cccgctgagg gcccaaagag ggcggcggct gattctgcaa gttgttcagc
     2941 cgtagcttct tggtctgatg gacggctcgg cgtccacatt ggccccgctg tttgcaaccg
     3001 cggcgatgac cggcaaaccg tgtgaccggt cttggaacgg cgatcgccgg gatgtccgct
     3061 ttcggttcat gcgcacgccc ctgggacgaa ccatcgtcca ggtgattgga ggcggtgcga
     3121 tcgtgttcgc ggttggattt ctgcaggggc gattcggccg ggcaattgac gcgggtgcag
     3181 ctaaaagcga agcgctcgaa ggtagtcgta cccgattcgg gcgcgctcga acggcgggac
     3241 acggccctca tcctgctcga cataaaggtg acgaactcct ccggtgcggg cctgcgccag
     3301 tatctgccgg aagtcgatga ttcctcggcc caactccgta aactcgtgtt gcggaccgga
     3361 agccatgtcc ttgaggtgga gcagcggaaa acgtcccggc cagcgggcaa gcaggtccac
     3421 ggggtttcga ccgcccttgg tagcccagta tacgtcgagc tcgagttgta ccagactggg
     3481 atcggttgcc tcgagcagca gatcgtacgg tacgtggcct tcgacccgtg caaactcgaa
     3541 atcgtggttg tgataggcaa attgaaggcc agcgcggtgc gccgcctgcc cgaccgtctt
     3601 gaacagcgcg atcagcgagt gccagtcgga cagcgaccgg cgttccgcgg gtggaaccca
     3661 gccgcagacc aggtaggtgt gaccgattgc cttggcacgg tcgatccggg acgcataggc
     3721 agtcctcaag tcggacgacg gaacttgagc cgaaggcgcg gtcagccccg cagtcgccac
     3781 cgccgcgcgg actgcattca ccgaccggcc gaagtaatcg gtgaactcga cttcacgata
     3841 gcccaggccg gcgatccgct ggagcgttcc ctcgaagtct cgggcaagct cgtcgcgaac
     3901 cgtgtacagc tcgagcccga tccgatcgag ccgatccttt gtcctccgga acacgcccgc
     3961 tgagcccgcc agagcaatcg tcccgacagc ggagacgaac tccctccgct tcacagctat
     4021 gctcgactct cagccactgg caacacttcc ccggccgcga gacggcgctc cagctcggcg
     4081 aacgccgcgt ccggatcgta tcctgagaag agcccgcgcg actgcttggt cgccagccac
     4141 cggccgaatc cggagtcgat cgcgccagcc gaggtctcct gccgctcggc cgcctcgacg
     4201 gcgaggtcgt tggcgtattc attcttgggg tgaccgcgat ggccgcgcac ccaggtccat
     4261 tgcacgtcgt gtaaggcgct cgaccgaacc agagcctgcc acaactccag gttttcgatc
     4321 tcgccgccct tacgtttcca cccccgtgcg gcccaccctg gcgcccattc cctgatccct
     4381 ttgaccaggt actccgagtc cgacaccatc agcgcgcgca ttcgcacgcc cttggcagcg
     4441 agcagctgca gcgctgcaat tgcgccgctc aacgccatgc gattgttggt tgtgtcaggc
     4501 gctgcgagaa agaagtcgcg ccgctggatg ccgctgcggg tgcgtacctc gatcaggcca
     4561 ccggccccgc caggcgtggt gccttcccgc ccgtttccga ggcaactctc atccaggtgc
     4621 aggacggcga tgggtgacgg acggggcatc aggttccctt gagtacctga aacgaggcga
     4681 tgtcgtccag tacaaagctc agctcttcac cggtcatcgg cagccgcgcg atcagggcct
     4741 cgcggggccc gtcggtggcg agtcgtgcgg caaccaccac atactcggtt cccttccgcg
     4801 tcgtgagcgc gacgcgcagg ccgtcgcgta cggcgcgttc cagttggtcc agtcgatcag
     4861 cggtccagcg cacgcttcat cactcgcacg tcgacgtcat gaggctacac cgccaggcgg
     4921 gttggccaga acctcgagcc tacctggatg atctgcggtg aggcggccaa ttgtgacggc
     4981 gaccggagtg ccccgagtct ggagggcttc gagaagcgcc ctttcgttct cggggggcac
     5041 ggcgagcagc agcccacccg atgtttgcgc atccactgcg agtaagcggt ccgtctccga
     5101 caactccgcc gcccagctga cccgctcatt ggcggcctcc agattgcgca tcgtcccgcc
     5161 ggggacgaag ccccgcgccg caagattgcg ggcatgcggg aatatcggaa ttgcgtcgaa
     5221 gtagagctcc ccaccgaccc ggctcgcctc aagcaggttg aggagatgtc ccacgagtcc
     5281 aaagccggtc acgtcggtcg cagcgctca
//
LOCUS       DASRJQ010000618         4088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2069897, whole genome shotgun sequence.
ACCESSION   DASRJQ010000618 DASRJQ010000000
VERSION     DASRJQ010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2069897"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="VFB89_14025"
     CDS             <1..815
                     /locus_tag="VFB89_14025"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HZM28461.1"
                     /translation="AFTDFCDRHYAGRWAWSTVDRAGFRGFLGELQRRGLSKRSAARA
                     LSAVRSLYRYLQTHHDLKGGAARSVRVPKLDKRLPTYLDLSRTETLFTWAEQRAESGD
                     FLALRDLAMLELFYSTGMRLSELAGLSLADVDLLSDQAKVRGKGRKERLVPVGSRAVS
                     ALRKYLRVRDEVVGQRGGDRRAMFVSVRGRRLPPRSIQRLMHRLFDAVGGDELRVHSL
                     RHTFATHLLNAGADLRAVQELLGHASLSTTQIYTHTSVERLKQVYQRAHPRA"
     gene            914..1453
                     /gene="hslV"
                     /locus_tag="VFB89_14030"
     CDS             914..1453
                     /gene="hslV"
                     /locus_tag="VFB89_14030"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HZM28462.1"
                     /translation="MAYHGTTILAVRKDGRLALGGDGQVTIGETVMKAKSVKVRTLKG
                     GKVLAGFAGSVADALTLYEKFEEKLDRFPGNLPRAAVELAKDWRSDRVLRRLEALLVV
                     ADLEHSYLLSGSGELIEPDDGILAVGSGGNYALAAARALLPDPQLSAREIVQRALDIA
                     ADICVFTNRNVTILELPTA"
     gene            1584..3065
                     /gene="hslU"
                     /locus_tag="VFB89_14035"
     CDS             1584..3065
                     /gene="hslU"
                     /locus_tag="VFB89_14035"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HZM28463.1"
                     /translation="MTKPVIQSEPAQPAEQQPLPWLEELAPRQIVAELDRYIVGQEAA
                     KKAVAIAVRNRWRRAQAPDEIRDEIMPYNIILIGPTGVGKTEVARRLARLAGAPFIKV
                     EASKFTEVGYVGRDAESMVRDLADSAINMVRTEREDEVYPQAEERADERLLDLLLPPP
                     PPQTPDAADKLKTAASVFIVSSKGQAKSERAATPDDERRQRSREKLRQMLAEGRLDDR
                     EVEIEVTAQGYPGLEMLQPPQGMEGADINFTEWLQEMLPKRQKRRTVKVPEARRVLVD
                     EELKKLVDMDDVVNEALDRVENHGIIFIDELDKIAGERGTSIGPDVSREGVQRDLLPI
                     VEGSTVQTRYGYVRTDHILFVAAGAFHVSKPSDLIPELQGRFPIRVELLPLTQEDFVR
                     IMTEPENALTKQYQALVAAEGATLEFTPDGIIEVARVAWLANDRMENIGARRLHTVMA
                     SLMEDVLFQLPNYPEKQIVFDAAGVKERLARIVNDDDLRRYIL"
     gene            complement(3170..>4088)
                     /locus_tag="VFB89_14040"
     CDS             complement(3170..>4088)
                     /locus_tag="VFB89_14040"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZM28464.1"
                     /translation="AGFTTVRNVGAGGFADVSLMKAIDAGLIQGPRIIPAGHAIGITG
                     GHCDATGWAPGVLELGPKEGVADGVDQVVAAARYQIKHGAKVIKVCATAGVFSYDATL
                     GAQQLSDAELRAVVEEASRHGLKVAAHAHGTDGIKAAIRAGVASIEHGSMLDDESIRL
                     MKEKGTYLVPTAYLLTQFKFEGIPAPIAAKAREAIPLAQESHRKAIAAGVKIAFGTDA
                     AIYPHGDNAREFAVYVGYGMKPIDAIRTATVNAADLLGVIDRGVIARGKLADLIAVPG
                     NPLQDVKMLEKVVWVMKGGVVYKGVNGER"
BASE COUNT          733 a         1258 c         1364 g          733 t
ORIGIN      
        1 cggcctttac cgatttctgc gaccgccact acgcgggacg ctgggcgtgg agtacggtcg
       61 atcgggccgg cttccgtggc tttctgggag agcttcaacg acgggggctg tcgaagcggt
      121 cagcggcgcg cgccttgtcg gcagtgcgaa gcctttaccg gtatctccag actcaccatg
      181 atctcaaggg cggggccgcg cgctcggttc gggttcccaa gctggacaag cgcctgccga
      241 cctatctgga tctctccagg accgagacgc tgttcacctg ggcggagcag cgcgcggaga
      301 gcggcgactt tttggcgttg cgcgacctgg cgatgctcga gcttttctat tcgacaggga
      361 tgcggctctc agagctcgcg ggattgagcc tggccgacgt cgatctgttg tcggatcagg
      421 cgaaggtgcg gggcaagggg cggaaggagc gcctggtgcc ggtcggctcg cgagcggtat
      481 ctgcccttcg gaagtatctt cgggtccggg acgaggtagt ggggcagcga ggaggggacc
      541 gacgggccat gttcgtgagc gtgcgtggcc ggcgactgcc tccccgcagc atccagcggc
      601 tcatgcaccg actcttcgat gcggttggtg gcgacgagct tcgggtccat tcgctgcggc
      661 acaccttcgc cacccatttg ctaaacgctg gagccgatct gagagcggtg caggaactgc
      721 tcggacacgc ctccctcagc accacgcaga tctacacaca taccagcgtg gaacggctca
      781 agcaggtgta tcaaagagcc catcctaggg cttgagaaga ctgcgtgaac ggtgaacggt
      841 aaacagtgaa cgggggcgcc cgttcaccaa ggcctcccgt tcaccgttta ccgtttaccg
      901 tttacgcttc tgcatggctt accacggcac gacgattctc gctgttcgga aggacggccg
      961 gcttgccctc ggcggggacg ggcaggtcac catcggcgag acggtgatga aggcgaagtc
     1021 ggtcaaggtt cgcaccttga agggcggcaa agtactggcc gggttcgccg gatccgtggc
     1081 tgatgcgctc actctctacg agaaattcga ggagaagctg gatcgctttc ccggcaacct
     1141 gccccgggcg gccgtggagc tggcgaaaga ctggcggagc gatcgagtgc ttcggcggct
     1201 cgaggcgctg ctggtcgttg ccgatctcga gcacagctac ctgctcagcg gctcgggtga
     1261 gctgatcgag ccagacgacg gcattctggc ggtcggttcg ggcgggaact acgcactggc
     1321 ggcggctcgg gcgctgcttc ccgatcccca attgagtgct cgtgagatcg tacagcgcgc
     1381 gctcgatatc gccgcggaca tttgtgtgtt taccaatcgg aatgtgacca ttctggagtt
     1441 gccaacagcg taaacggtga acggtaaacg gtgaacggga acttctagga gcagcgagcg
     1501 gaccccgggt agccccgcga gactgcttcg agcgactgat ccccccgttc accgtttacc
     1561 gttcaccgtt tacgttggtt ccaatgacca agcccgtcat ccagtccgaa ccggcccagc
     1621 cggctgagca gcagccgctc ccgtggctcg aggagctggc gccgcgacag atcgtcgcgg
     1681 agctggatcg ctacatcgta ggccaggagg cggcgaagaa ggccgtcgcc atcgcggtcc
     1741 gcaaccgctg gcgtcgggcc caggcgccgg atgagatccg cgatgagatc atgccgtaca
     1801 acatcattct catcgggccg accggcgttg gtaaaacgga ggtcgctcga cgtctggcac
     1861 gtcttgccgg tgcgccgttc atcaaggtcg aagcgtccaa gttcaccgaa gtgggctatg
     1921 tcggtcgcga tgcggagtcc atggtgcggg atctggcaga ctcggcgatc aatatggtcc
     1981 gcaccgagcg ggaggacgag gtctacccgc aggccgagga gcgggcggac gagcggttgc
     2041 tggatctgct gctgcccccg ccgccgcctc agacgccgga cgcagccgac aagttgaaaa
     2101 ccgcggcttc cgtcttcatc gtgtcatcca aggggcaggc caagtcggag cgggcagcga
     2161 cgccggatga cgagcggcgc cagcggtccc gggagaagct gcgccagatg ctggccgaag
     2221 gtcggctcga cgatcgagag gtcgagatcg aggtgaccgc gcagggctac cccggcctcg
     2281 agatgctcca gccgccgcaa ggcatggaag gcgccgacat caacttcacc gaatggctgc
     2341 aggagatgct gcccaagcgc cagaagcggc ggaccgtgaa ggttcctgag gcccgtcgcg
     2401 ttctggtgga cgaggaattg aagaagctgg tggacatgga cgacgtggtg aacgaggctc
     2461 tggatcgggt cgagaaccac ggcatcatct tcatcgacga actggacaag atcgccggtg
     2521 agcgagggac ttcgatcggc ccggacgtct cccgcgaagg cgtgcagcgc gacctgctgc
     2581 ccatcgtcga gggctccacg gtgcagactc gatacggcta cgtccggacc gaccacatcc
     2641 tgttcgtggc tgcgggcgcg ttccacgtgt cgaagccgtc cgacctcatc ccggagctgc
     2701 agggccgatt tcccatcagg gtggagctac tgccgctgac tcaggaggac ttcgtccgga
     2761 tcatgaccga gccggagaac gcgctgacca agcagtatca ggcgctagta gcggcggagg
     2821 gggcgaccct cgagttcaca cccgacggca tcatcgaggt cgcgcgggtc gcctggctcg
     2881 ccaacgatcg catggaaaac atcggtgctc gccgcctgca tacggtgatg gcgagcctga
     2941 tggaggacgt gctcttccag ctgccgaact atcccgagaa gcagatcgtc ttcgatgcgg
     3001 ccggggtcaa ggagaggttg gcgaggattg tgaatgacga tgatctacgc agatatatac
     3061 tgtaaagcgt gaacggtgag cggtgaacgg gagttcttaa gagcggtgag cgggcgtgcc
     3121 ccgggtagtc ccgcgaggcc gctccgaacg actgacagcc cgttcaccgt taccgttcac
     3181 cgttcacgcc cttatacacc actcctcctt tcatcaccca caccaccttc tccagcatct
     3241 tcacgtcctg cagcggattg ccgggcacgg cgatcagatc cgccagcttg ccccgagcga
     3301 tgacgccgcg gtcgatcacg ccgagcaggt ccgcggcgtt caccgtggcg gtgcggatgg
     3361 cgtcgatcgg cttcatccca taacccacgt acaccgcgaa ctcgcgggcg ttgtccccgt
     3421 ggggatagat ggccgcatca gtgccgaacg cgatcttgac gccggctgcg attgccttcc
     3481 gatggctttc ctgcgccagg ggaatcgcct cccgagcctt cgctgcaatg ggtgcgggga
     3541 ttccctcgaa tttgaattgt gtgaggagat aagcggtggg gacgagatag gtgcctttct
     3601 ctttcatcag ccggatcgat tcgtcatcca gcatcgagcc gtgctcgatc gaggcgacgc
     3661 cagcccgaat ggcggccttg atgccgtcgg tgccgtgcgc gtgtgccgcg accttgaggc
     3721 cgtgtcgact cgcttcctcg accacggcgc gcagctccgc atcggagagc tgttgcgcac
     3781 ccagggtagc atcgtaggag aacacgccgg cagtcgcgca caccttgatg accttggcgc
     3841 cgtgcttgat ctggtagcgt gcagcggcga ccacctggtc gaccccgtcc gccacgcctt
     3901 ccttggggcc cagctcgagc acgcccggtg cccatccggt ggcgtcgcaa tgacctccgg
     3961 taataccgat cgcgtggccg gccgggatga tgcgggggcc ctgaatcagt ccggcgtcta
     4021 tcgccttcat cagagacacg tcagcaaacc cgcccgcgcc cacgttgcgg acggtggtga
     4081 agccggcc
//
LOCUS       DASRJQ010000619         4187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2641547, whole genome shotgun sequence.
ACCESSION   DASRJQ010000619 DASRJQ010000000
VERSION     DASRJQ010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2641547"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VFB89_14045"
     CDS             complement(<1..321)
                     /locus_tag="VFB89_14045"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZM28465.1"
                     /translation="MARAIPSTEIEPGTVATEAGNPDLVVLSAQPFCAEAAPPSLVAR
                     LTAGPGVFVRTNFNVPALDPSTHTIDVGGAVARALTLSVADLRGFGLTTIEATLECAG
                     NGRAT"
     gene            517..2070
                     /locus_tag="VFB89_14050"
     CDS             517..2070
                     /locus_tag="VFB89_14050"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HZM28466.1"
                     /translation="MASDTSIEILLDVWREACRHLAVEDAFERIAALVGSRLPADVLV
                     VRRVDPERRRIETVPAGHADRGSQGSVERTECTAQQLDELARWARRGAVLRCGPLTKH
                     PLAPVIAPRGIWADVMAGPLLDEERVIGLLVAAVAPPRQFTAEHESLLSRLLEPCLVA
                     LVNDARWHELVRLREASEADRRALLSRLERDSLSDSIVGADAGLREVMERVEQVAPTD
                     APVLLLGETGTGKEVIARAIHARSRRAGGPVVRVNCGAIPPGLIDSELFGHVRGSFTG
                     AVAERKGWFERADGGTLFLDEIAELPLDAQVRLLRILQDGTYERVGGQRTLHVDVRIV
                     AATHRDLRERVTEGSFREDLWYRIGVFPLHLPALRDRLEDLPALAAHFAWRAGMRLGG
                     HPLAPTPHDIELLVDYPWPGNVRELAAVIERAAILGDGRTLDIAHALGGPPEPTDSSA
                     AVTRSPSEDLVPLDDAIRRHIIQALRVTGGRVEGSRGAAVLLEVNPHTLRARMRKLGI
                     DWARFRAGV"
     gene            complement(2067..3650)
                     /locus_tag="VFB89_14055"
     CDS             complement(2067..3650)
                     /locus_tag="VFB89_14055"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HZM28467.1"
                     /translation="MTATEPVAGVARTPPTLEARERLAAIDVGSNSIRLLVAEYDPQS
                     GISVIDEVKDQPRLAQGLTGTGRLDERAMARALATLARMREVCRRRGANRIAAVATAA
                     VREAINGEAFVKRVRDELDIPLRIIDAETEAALSYRSVAHHFRVAGARTLVADIGGGS
                     LELIGAVDGLVELTRSLPLGAVRLTETHLPGKRDSRREVASLRTEIRRQLKRGFRVRE
                     WTAATVIGSGGTFTTLGRMATARRGLPVPDTVHGVSVATAEVETLLEWLAAKTPEQRR
                     MVAGLNPQRADIISAGLAVTAELLELLSAPTVTVSAFGLREGILLEMVDAHAVPAPRD
                     PLRLMREFVERCQGDRRHVEHVRVLARQLFDLLSQPLGAAPDEWLILEAASLLHDVGQ
                     LVSYRKHHRHSYQLIMHAERLDLSARDRGLVALVSRYHRRKGPSRKHPEFAALNAEDQ
                     GVVRRMSALLRVADGLDRGHTSNVDRVTVALTPEEVAIEVAPRVAGADLSLECWGAER
                     KSNVLAKVLERDVVIRTAA"
     gene            complement(3647..>4187)
                     /gene="phoU"
                     /locus_tag="VFB89_14060"
     CDS             complement(3647..>4187)
                     /gene="phoU"
                     /locus_tag="VFB89_14060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HZM28468.1"
                     /translation="MEMEIEEMSVNLLALQQPMARDLRMLTSALKIANDLERVGDHAV
                     NIAQSAERLSQARPIAPEPEIVEMARLAREMLSDALEAFIRGDATAGREICLRDDKVD
                     ALHRSVFRILLTHMMEDPHTIGAGMELFLVSRNLERVADLATNIAEDVVFLVEGKSIK
                     HHAEDRGEARTSTGVSVPS"
BASE COUNT          697 a         1370 c         1400 g          720 t
ORIGIN      
        1 cgttgcgcgg ccattgccgg cgcattccag ggtcgcttcg atggtagtga gaccaaagcc
       61 gcgaagatcc gcgaccgaga gggtcagcgc gcgagccacc gcaccaccga catcgatagt
      121 gtgagtgctc ggatcgagcg cagggacatt gaagttggtg cgaacaaaaa ccccggggcc
      181 ggcggtcaac cgcgccacca gggagggcgg tgcggcctcg gcgcaaaagg gttgagcgga
      241 caggaccacg agatccggat tccccgcctc cgttgcgacc gttccgggtt cgatctcggt
      301 gcttggaatc gcccttgcca tgagattccg catcaaagtg acacagaaag acatggcaga
      361 gggcatgccc actcgggttg ccagcacatc catcgtcagc acaaagactt agcacaattc
      421 agctcgacgc ctgttgcgcg aagcccgtca aatacgatgg agggctctgt tattagtgct
      481 ggttaaggcc agcagcccgt gatatagtat ggcccaatgg cctccgacac ctccatcgaa
      541 attctcctcg acgtatggcg ggaagcctgc cgacacctcg ccgtcgaaga cgcattcgag
      601 cgaatcgccg cgctggtcgg ctcccggctt ccggccgatg tgctggtggt ccgtcgagtc
      661 gatcccgaga gacgccgcat agagacggtt cccgccgggc acgccgaccg cgggtctcaa
      721 ggatccgtcg agcgtactga atgcacggct cagcagctgg acgagttggc gcgctgggcc
      781 cggcgcggag cggtgcttcg ctgcggtcca ctgaccaaac atcccctggc cccggtcatc
      841 gcaccccgag gaatctgggc cgacgtgatg gccggcccgc tgctcgacga ggagcgggta
      901 atcggattgc tcgttgcggc tgtcgctccc ccccgccaat tcactgcgga gcacgagtcg
      961 ctgctgagcc gtctgctcga gccgtgctta gtagccctgg tcaacgacgc ccggtggcac
     1021 gagctggtcc gtctacgcga ggcatcggag gcagaccgac gtgcgctcct gtcgcgcctt
     1081 gagcgcgact cgctttcgga cagcatcgtc ggcgccgatg ccggattgcg tgaggtgatg
     1141 gagcgcgtgg agcaggttgc gcccaccgac gccccggtgt tgctgctggg agagacgggg
     1201 accggcaagg aggtcatcgc gcgggcgatc catgctcgtt cccgtcgggc aggcggacct
     1261 gtcgttcggg tcaactgcgg tgcgattccg ccggggctga tcgactccga gctattcggc
     1321 cacgttcgcg gaagctttac cggcgcggtg gcggagcgca aaggctggtt cgagcgggcg
     1381 gatggcggaa ccctgttctt ggatgagatc gccgagctgc cgctcgatgc gcaggttcgc
     1441 cttctcagga ttttgcagga cggcacctac gagcgcgtag ggggccagcg caccttacac
     1501 gttgatgttc ggatcgtcgc ggcgacgcat cgcgacctgc gggagcgcgt cacggaagga
     1561 agcttccgcg aagatctgtg gtatcgaata ggcgtgttcc cgctccatct gccggcgttg
     1621 cgtgaccggc tggaagacct gcctgcgctc gccgcccatt ttgcctggcg agccggcatg
     1681 cggctggggg gccacccgct cgctccgacg ccgcacgata tcgagctgtt ggtcgactac
     1741 ccctggccgg ggaacgttcg tgagctggcg gcggtaatcg agcgggcagc catcctcggc
     1801 gacggaagaa cgctcgatat cgcgcacgcg ctgggaggac cgcccgagcc aacggatagc
     1861 agcgcagccg taacccgcag tccatcggag gatctggtgc cgctggacga cgccatacgg
     1921 cggcacatca ttcaggcgct tcgagtgacg ggaggacggg tggaaggaag tcggggggcg
     1981 gcggtgctgc tcgaggtgaa cccgcacacg ttgcgagccc ggatgaggaa gctgggaatc
     2041 gactgggctc gcttccgggc aggagtctag gcggcagtcc gaatcacgac atcccgttcc
     2101 agcaccttcg ccagaacatt cgatttccgc tcggcgcccc agcactcgag cgagagatcg
     2161 gctccggcca cgcggggcgc cacttcgatg gcgacctctt cgggcgtcag cgctaccgtg
     2221 acccggtcga cattcgaggt atgtccccga tcgagcccgt ccgccaccct gagcaatgcg
     2281 ctcatccgtc gcaccacacc ctgatcttcc gcgttgagcg cggcgaactc gggatgtttc
     2341 cggctggggc cctttctccg atggtagcgg ctcacgaggg cgacgagacc gcggtccctg
     2401 gccgagagat cgaggcgctc ggcgtgcatg atgagctgat agctgtggcg atggtgtttg
     2461 cgatagctga ccagctggcc cacatcatga agcaggctgg cggcctccag gatcagccac
     2521 tcgtcgggcg cggcgcccag gggttggctc agcagatcga acagctgccg cgcgagcacc
     2581 cggacgtgct ccacgtgacg gcggtcgccc tggcatcgct cgacgaattc ccgcatcagg
     2641 cgcagcgggt cccgtggggc agggaccgca tgcgcgtcca ccatctcgag cagaattccc
     2701 tcgcgcagcc caaaggcgct cacggtcacc gtcggggcgc tgagcagctc cagcagttcg
     2761 gccgtcaccg ccagtccggc gctgatgatg tccgcccgtt gagggttgag cccggcgacc
     2821 atccgtcgct gctccggcgt cttggcggcg agccattcga gtagcgtctc aacttccgca
     2881 gtcgcaacgc tcacgccgtg gaccgtatcc ggcaccggaa gacccctgcg cgcggtagcc
     2941 atccgcccga gtgtggtgaa ggtgccgccg gagccgatca cggtggcggc ggtccattcc
     3001 cgcactctga agccgcgctt gagctggcga cgaatctcgg ttcgcagcga agcgacttcg
     3061 cgccggctgt cccgctttcc gggcaggtgg gtttcggtga gccggacggc gccgagcggc
     3121 agcgagcgag tcaattcgac caggccgtcc accgcgccga tcaactcgag actgccgccc
     3181 ccgatatcgg caaccagcgt gcgcgctccc gccacgcgga agtggtgcgc gaccgagcgg
     3241 tagctgaggg cagcctcggt ctcggcgtcg atgatccgaa ggggaatgtc gagctcgtct
     3301 cgaacccgct tgacgaaggc ctcgccgttg atggcttccc gtaccgcggc agtcgcgacc
     3361 gccgcgatcc ggttggctcc tcgacgccgg cagacctcac gcatgcgggc gagtgtagcc
     3421 agcgcgcgcg ccatcgcccg ctcgtccagc cggccggtgc cggtgaggcc ctgggccagg
     3481 cgaggctggt cctttacttc atcgatgacc gaaattccgc tctgcggatc gtattcggcg
     3541 acgagcagac ggatcgaatt ggagccgaca tcgatcgctg cgaggcgctc ccgagcctcg
     3601 agggtgggag gtgttctcgc gactccagcg accggctcgg tcgccgtcat gacggcacgc
     3661 ttactccggt ggaggttcgt gcctcacccc gatcctcggc gtgatgcttg atggacttgc
     3721 cttcgaccag gaatactacg tcttcggcga tgttggtcgc gaggtcagcc acccgctcca
     3781 gattccggct caccaggaac agctccatac cggcgccgat ggtatgggga tcttccatca
     3841 tgtgcgtgag cagaatccgg aacaccgacc gatgcagggc atccacctta tcgtcccgga
     3901 gacaaatctc ccggccggcc gtcgcatctc cccggatgaa tgcctccagc gcatccgaca
     3961 gcatctcgcg ggcgagccgg gccatttcga cgatctccgg ttcgggcgca atgggccggg
     4021 cctggctcag ccgctcggcc gactgcgcga tgttgaccgc gtggtcgccg acccgctcga
     4081 ggtcattcgc gatcttgagt gccgacgtca gcatccgcag gtcgcgggcc atgggctgct
     4141 gcagcgcgag gagattgacc gacatttcct cgatctccat ctccatg
//
LOCUS       DASRJQ010000620         4228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_4115485, whole genome shotgun sequence.
ACCESSION   DASRJQ010000620 DASRJQ010000000
VERSION     DASRJQ010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4115485"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..1207
                     /locus_tag="VFB89_14065"
     CDS             203..1207
                     /locus_tag="VFB89_14065"
                     /inference="COORDINATES: protein motif:HMM:NF016155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HZM28469.1"
                     /translation="MHTSPIFFMLFTAGACVMWIFSLLGALRRRPYYAFVLVFMTAYC
                     FCSEFLAIRLGKYYYGPFLLEICGNEPTGHLPWPLSLLQYGGCLAPNWNIPIGVIALE
                     SSMFMAILWTADLLTSSPSVKPLLAALMGVNLDAIIDPVAASSTWCSKELGGVSFVGL
                     GFWTWFTTEQNPGYWFGVPILNYTTWFISMAAFAFAVHSMERRILGDEHGVLSQLWAA
                     LSAMVGLLVVEFLLVLTVQTVLDLHPDLAWQAGILLAMIGLSLLVVLPAMRGFNRANP
                     RDWTTLAPQLFPLVFFPAALVIHGGFPHKGWLYLVAPVTFLIGAAFAFAPYSKPGARR
                     "
     gene            1204..2301
                     /locus_tag="VFB89_14070"
     CDS             1204..2301
                     /locus_tag="VFB89_14070"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HZM28470.1"
                     /translation="MNPIAPVIRGCGRAWARFLALDRVIRVHFLFFSSFLPLLGGSTA
                     RRDLDPAQILALLGVGLCFHLYSYVLNDVCDLPVDRTHPARQNDPLVRGTLRTWQALL
                     IALVPVPLTVPLTVWLGGGARAYAMLGAGFALMAVYNLWGKRCPIPPLTDAAQGLAWG
                     SLALYGCLAIGGDPNVLTWLVTLYGAGFLLHINGIHGGLRDLENDLASGARTTAIYLG
                     ARPRPGGEVHVPSGVSVFAYSVLVGLAAMLLVPLLRNDFGYEPGVWTAMLAIAGSLLF
                     VDLLLLAIVMRPNRPRWNVVFRVHCFSVAAVLPITFAPFLSAETLAALLLVLVLSFLP
                     LEWARWLRPAYPSPTERNAAQRPAPQEIAAE"
     gene            complement(2242..>4228)
                     /locus_tag="VFB89_14075"
     CDS             complement(2242..>4228)
                     /locus_tag="VFB89_14075"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZM28471.1"
                     /translation="TPGVDLTQSGLYDFNLNARGFNSTLTRRILTLVDGRDTSTVFTG
                     TQEWSAISFPLDELSRVELVRGPGSALYGANAFSGVLNMTTKPPRGNEGGQIRLAFGE
                     LDTRRADFRFAADLGAGYYGKLVGGYTESGDFTASRRIQAEYLPCPAGLTTGCLGRER
                     LDPRLKEDKIAFGGLRLDKYFGDSMVLTVEGGTAEIGGPTVVTGAGRVQITDSARPWA
                     RLNVNAPQWNLLSYYNRRKSEDQLSLPSGALLFEDSTKRHAELQGNRAFGRGKGFAVI
                     GLSYHQQETDTANHQGVQTLLARPQSERQEAVFGQVEHSFGNHLKAVVAARADRSTLH
                     DTQISPKGALVWSIHPNHTLRLTYNRAFQQPNISERFLQARTSFPGTTLSALDLSAIE
                     TGLAPFLGGVSLGFSGIPVLVLGNEDLEVEKIRSYEAGYHGILGSNAYITIDCYQSEI
                     EDFVTDLLRNVGPGGRTNPDFGPYAPPASLPEPVRRQILEILRTNPATAAVFPFLSNL
                     ANGQPIVALASYKNAGRVETHGVDVALHYSFLERWLLDVSYSWFDFDVREQALGDQLL
                     PNAPEHKATAGLSYSHGRTSAAIAYRWVDDFSWAAGSYTGPVPSYGVADLSASYRLSD
                     RWEIGLNVANLFDHEHWQSFGGDLLRRRALGSVAFRW"
BASE COUNT          641 a         1391 c         1351 g          845 t
ORIGIN      
        1 atcgccgatc ctgcacgctc cacccatctg ctggctgccg aggagggttt tcggcgcatg
       61 cgcgccgagc ccgacctgag ccgggtcgcc gccgccctcc aggagagctg accgcggcga
      121 gccgcgcgtg atagactcga agcatccgag cagcttggat tcttgccaat ttgcggctgg
      181 ctttcgcagt aaaggaaaga gcatgcatac gtctccaatc ttcttcatgc ttttcacggc
      241 aggtgcgtgc gtgatgtgga ttttcagcct gctcggcgca ttgcggcggc ggccctatta
      301 tgccttcgtc ctcgtcttca tgacggccta ttgcttctgc agcgagttcc tcgccatccg
      361 gctcggcaag tactactacg ggccgttcct gttggagatc tgtggcaatg agccgacggg
      421 ccatctaccc tggccgctca gcctcctgca atacggcggc tgcctggcgc ccaattggaa
      481 catccccatt ggcgtcatcg cgctggagag cagcatgttc atggccatcc tctggacggc
      541 cgacctcctg acttcctcgc cgtctgtgaa gccactcctc gccgcactga tgggtgtgaa
      601 cctcgacgcc atcatcgacc cggttgcggc ttcgagcaca tggtgctcca aagagctggg
      661 cggcgtgagc ttcgtgggcc ttggcttctg gacctggttt accaccgagc agaatcccgg
      721 gtactggttc ggcgttccaa tcctcaacta tacgacctgg tttatcagca tggccgcctt
      781 cgccttcgcg gtccactcca tggagcgccg catcctcggg gatgagcacg gggtgctgtc
      841 gcagctctgg gccgcgctgt cggccatggt cggcctcctc gtcgtggagt tccttctcgt
      901 tctcaccgtg caaaccgttc tcgatctgca tcccgatctc gcttggcagg caggcatcct
      961 gttggcgatg atcgggctca gcctgctcgt ggttctgccg gcgatgcgcg gcttcaaccg
     1021 agcgaatcca cgcgactgga cgacgctggc gccgcagctc ttcccgctcg tcttctttcc
     1081 ggccgctctg gtcatccacg gcggtttccc gcacaaaggc tggctctatc tcgtcgctcc
     1141 cgtcaccttc ctgatcgggg cagcctttgc ctttgcaccc tattccaagc cgggagcgcg
     1201 acgatgaacc cgatcgctcc agtgatccgt ggatgcggcc gcgcctgggc caggtttctc
     1261 gcgctggatc gggtgatccg cgtgcatttc ctgtttttct cgagctttct gccgctcctc
     1321 ggcggttcca cggcacgccg cgacctcgat ccggcgcaga tcctggcgct cctcggggtc
     1381 ggcctctgtt ttcacctcta cagctacgtc ttgaacgacg tctgtgatct gccggtcgac
     1441 cgcactcacc cggcgcgcca gaacgatccg ctggtccgcg gcaccctgcg cacctggcag
     1501 gcgttgctga ttgccctggt gccggtcccc ttgaccgtgc cgctgacggt ctggttgggc
     1561 ggcggcgctc gagcctacgc aatgctcggc gccggcttcg ctttgatggc cgtctacaac
     1621 ctgtggggca agcgctgccc catcccgccg ctcaccgatg ccgcgcaagg cctcgcgtgg
     1681 gggagcctcg ccctctatgg ttgcctggcg atcgggggcg atccgaacgt tcttacctgg
     1741 ttggtcacgt tgtatggggc agggttcctt ctgcacatca acggcattca tggcgggctg
     1801 cgcgacctgg agaacgatct ggcgagcgga gcccgtacca ccgcgatcta cctcggcgcg
     1861 cggccccgtc cgggtggcga ggtccatgtg ccgtccgggg tctcggtctt tgcctattcc
     1921 gttctcgtcg gcctcgccgc gatgcttctc gtgcccctgc tgcgcaacga cttcggatac
     1981 gagcctggcg tgtggaccgc gatgttggcg atcgccggct cgctgctctt cgtcgatctc
     2041 ctgctgctgg cgatcgtcat gcgccccaac cggccgcggt ggaacgtcgt gttccgcgtg
     2101 cattgcttct ccgtcgcggc ggtgcttccc atcaccttcg ccccgttcct cagcgcggaa
     2161 accctggcgg cgctcctgct ggtgctggtg ctctctttcc tgccgctcga atgggccagg
     2221 tggctgcggc cggcgtaccc ctcaccaacg gaacgcaacg ctgcccaacg cccggcgccg
     2281 caggagatcg ccgccgaatg attgccagtg ctcgtggtcg aaaagattgg cgacgttgag
     2341 gccgatttcc cagcggtcgc tcagcctgta gctggcactg aggtcggcca cgccgtagga
     2401 tggcacggga cctgtgtacg atccggccgc ccaggaaaaa tcgtcgaccc agcggtatgc
     2461 gatggcggcg ctggtgcggc cgtggctgta ggagaggccg gcagtggcct tgtgctccgg
     2521 cgcattgggc aggagctggt cgccgagcgc ctgctcccgg acgtcgaagt cgaaccatga
     2581 atagctgacg tccaagagcc agcgctccag gaaggagtag tggagcgcca cgtcgacgcc
     2641 atgcgtttcc acgcggcctg cattcttgta ggaggccagg gccacgatgg gctgaccgtt
     2701 ggcgagattg gagaggaacg ggaagaccgc cgccgtggcc ggattcgtgc gcaggatctc
     2761 caggatctgg cgccgcaccg gctcgggcag cgaggcggga ggcgcgtacg gtccgaaatc
     2821 cgggttcgtc cgcccgccgg gaccgacgtt gcgcagcaag tcggtgacga agtcctcgat
     2881 ctcgctctga tagcaatcga tcgtgatgta agcgttggat ccaaggatgc cgtgatagcc
     2941 ggcctcgtag ctgcggatct tctcgacctc cagatcctca ttgccgagca cgagcaccgg
     3001 gatccccgag aagccgaggc tcacaccgcc gaggaacggc gcgaggccgg tctcgatcgc
     3061 cgacaggtcg agcgcggaga gggttgttcc ggggaaggag gtcctcgcct ggagaaagcg
     3121 ctcggaaata ttcggctgct gaaaggccct gttgtaggtg agccgcaacg tgtgattggg
     3181 gtgaatggac cagaccagag ctcctttcgg cgaaatctgg gtgtcgtgca acgtgctccg
     3241 atccgcgcgc gcggcgacca cggctttgag atgattccca aagctgtgct cgacctggcc
     3301 gaacacagcc tcttgcctct cgctttgagg cctggccaag agcgtctgga cgccctgatg
     3361 attcgcggta tcggtctcct gctggtgata agagagcccg atcaccgcga aacccttgcc
     3421 gcgcccgaag gcccggttcc cttgtagctc cgcgtgccgc ttcgtggaat cctcgaaaag
     3481 cagcgcgccc gaagggaggc tgagctggtc ctcggatttg cggcgattgt aatagctgag
     3541 caggttccac tgcggagcat tgacgttgag gcgagcccaa ggccgcgccg agtcggtgat
     3601 ttgaacgcgg ccggcgccgg tcaccacggt cggaccgcca atttcggccg tgcctccctc
     3661 gaccgtcagg accatgctgt ccccgaaata tttgtcgagc cgcaggccgc cgaaggcgat
     3721 cttgtcctct ttcagccgcg ggtcgagccg ctcgcggccc aggcagcccg tcgtcaggcc
     3781 cgccgggcac gggagatatt cggcttggat cctgcgtgac gccgtgaagt cgccactctc
     3841 ggtgtagccg ccgacgagct tgccgtaata gccggcgccc aggtccgcgg cgaaccggaa
     3901 gtcggcacgc cgcgtgtcga gctcgccgaa cgccagccgg atctgccctc cctcgttccc
     3961 gcgcggcggc ttggtcgtca tgttgagaac gccgctgaag gcgtttgcac catagagcgc
     4021 cgatccggga ccccggacca gctcgacgcg cgacagctca tcgagaggga acgagatcgc
     4081 ggaccactcc tgcgtgcccg tgaagaccgt agacgtatcc cggccatcga cgagcgtcag
     4141 gatccggcgc gtcaaggtcg agttgaagcc gcgggcgttc agattgaagt cgtagagacc
     4201 gctctgggtg agatccacgc ccggcgtg
//
LOCUS       DASRJQ010000621         7537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1230456, whole genome shotgun sequence.
ACCESSION   DASRJQ010000621 DASRJQ010000000
VERSION     DASRJQ010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230456"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VFB89_14080"
     CDS             complement(<1..250)
                     /locus_tag="VFB89_14080"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZM28472.1"
                     /translation="MKVYGSTAIRNVAFVGHGGSGKTSLVDALAFVSGSSRRHGSIKE
                     GTALTDYSPDEIERKHSISLGLAYAEWLDTKINLIDTPG"
     gene            complement(393..2276)
                     /locus_tag="VFB89_14085"
     CDS             complement(393..2276)
                     /locus_tag="VFB89_14085"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZM28473.1"
                     /translation="MSLPLAHPIVRPNVGGRKPDAPTPSWGERLRAIRHLRTLLRLVW
                     STEPKYVVGILLLRMVRAGVPVAVLWVGKLIVDQVVAAIQQRQVPWSHLGVLLGIEFS
                     IAVIGEGLSRLSALLESLLGDLFANRTSVDLMRHSATLDLEQFEDADLYDKLERARRQ
                     TVNRIGLFTLLLSTLQDLITLVTLSAALVAYVPWLLVLLALAVVPSLLGETHFASLGY
                     SLLYSWTPERRLLDYLRYAGASDVSAKEVKLFGLSDFLVGRYDRLSREFYEANKALAV
                     RRSLVSSLLATVGTLGYYGAYVVIIYLTVIGHRGPAGLFTIGVLTFLTGSFRQSRDLI
                     QRVSLSLTQVFEQSLYLDDLFSFFALEPRVRNVPNARPVPVPIKRGLVFEDVGFHYPG
                     SDTWAVRHLDFALEPGERIALVGENGAGKTTLAKLLVRLYDPTEGRILLDGVDLRDYD
                     IDSLRRNVGVIFQDFVRYDFIMRENIAVGNIDRLEDQARIQGAAERSLADSVAGRLAG
                     RYDQMLGRRFEGGVELSGGEWQKVALARAYMRDAQILILDEPTAALDARAEYQVFLRF
                     SELTAGKMAVLISHRFSTVRMADRILVLRAGELVEQGTHEELVARGGLYAELFQLQAA
                     GYR"
     gene            2311..2922
                     /locus_tag="VFB89_14090"
     CDS             2311..2922
                     /locus_tag="VFB89_14090"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HZM28474.1"
                     /translation="MEPHLLSVWHDLVRLGTPEMANDWAHWLEKLIRPAVVYLFLVFL
                     LRGAGRRMLAQLNPFDFVVLLTLSNTVQNAIIGEDSSLLGGIVGALGLMGVNALLIRH
                     YYRGPSQDRLSDTDVDIPLIHGGELAESELRRFHINAGELVAKAHERGFDSLGEVESA
                     MLYPNGTVYFRARHPDEAERRHRQLMERLDHLQREVASLRISH"
     gene            2943..3668
                     /locus_tag="VFB89_14095"
     CDS             2943..3668
                     /locus_tag="VFB89_14095"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZM28475.1"
                     /translation="MPDTPIPEPRTALVTGATDGIGRATAFALGRAGWQVGCCARTAG
                     KVEALLDQLAEAGIRAGGRPADVGEEREARNAVRHVVDTLGPIDALINNAGILIAKPF
                     EQLTLADWDHTMATNLRSLYLMTREVLPDMRRRRRGAIVNVASLAGRNGFVGGTAYTA
                     SKHAVLGFSRSLMLEVRKDDVRVIAICPGSVDTGLLHDQPLLKTDPTRILRPEDVAAV
                     IVHALELPARALVSELDIRPTNP"
     gene            complement(3722..5218)
                     /locus_tag="VFB89_14100"
     CDS             complement(3722..5218)
                     /locus_tag="VFB89_14100"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZM28476.1"
                     /translation="MNEAPDLRPLPLIPLLRAELGAPDQAVLATWHEALADSIGVELP
                     HDLLGLWLYPAAGGAVLIGPEALSQDRLPVPLPQPRVSREQAEELASIVRRAGYQSAE
                     CLPIRFGRTDVGLLLIAALEANRYDEAAPGLLERIGEAIAPMLARIAQQRSTDAEAAA
                     PDLGAELANAWIEARSPRDFFGLASQVLGRVVPHDLFEVLIPGPAPGQQYRLGAHPDG
                     PPWAEANLIIGKHQLDLFALFAGEPTLDLADVAADSRWPADLLVDELPPGQRLRSVIG
                     VRVISSGHLAGHLLVGSTRPRFFSTGDLEMLVRLGHILGPKIDGYVLTSQLHVLRKQL
                     VTLRSAPAHFARVADMLATTAQFAEATRRLAEETRSMMPCERLTLAVRLTEADRVVLI
                     EPGENKHWSDLPLVPVSGTPLGQVLAGHLPDLMSETRTGAELIVPLRVSGKTIGALVL
                     AARGFGSISRADVAPVQQLADLVAPYVELVRRAVMLPMPVMPGWKRVN"
     gene            5444..5662
                     /locus_tag="VFB89_14105"
     CDS             5444..5662
                     /locus_tag="VFB89_14105"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HZM28477.1"
                     /translation="MTQATLHIQGMSCAHCVNAVRRALSTLRTVEITSLQLGRADLTF
                     DETVVTPDRLTAVVSDAGYIAQFGNVGR"
     gene            5649..>7537
                     /locus_tag="VFB89_14110"
     CDS             5649..>7537
                     /locus_tag="VFB89_14110"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HZM28478.1"
                     /translation="MSAASATCTIPVTGMTCAACSGRVQRSLERAAGVSSATVNLMTG
                     EATVAFDPQAIAPDQLVQVIRDTGYGASLRVSPTDAEDSLERTEREREAEVTTLKRKL
                     WVALLAAAFLIVQGHPHTSAARWIALALTLPVVGWSGRHFYTRAWMALRHGGADMNTL
                     IAVGTAAAFGFSVFMTAAAKWVAAHGIALEVYYETVATIIALVLLGNVLEARATSRTS
                     SAVRKLMGLRPTTARVIRGNAEAEVAIGQVRVGDEVVVRPGERIPVDGIVIRGSSTVN
                     ESMLTGEPAPVVKELGAEVVGATLNRHGALYVRATRIGADTVLARIIALVRGAQGQKA
                     PIQRLADRISAVFVPIVLAIAIITFALWYMLGPEPRYLHALVAAVTVVIIACPCAMGL
                     ATPTAVMVATGRGAELGVLIKGGGPLQRAAEVDTVVLDKTGTVTEGRPQVVQLDLGND
                     ALGLLGSLEQLSEHPLGETIVGAARSRGLILRTPESFGVRPGKGVVGTVDGRTVVAGN
                     RALLAENGVDASDLDSAAVKLSEQAATPVFVAVDGHPAGLVGIADPVKPTSAEAVKQL
                     DALGLDVVMLTGDESRTAAAVARKVGISRVLADVLPQQKLNEVRRIQGDGRVVAMVGD
                     GLNDAP"
BASE COUNT         1439 a         2452 c         2387 g         1259 t
ORIGIN      
        1 atccgggggt atcgatcagg ttgatcttgg tatcgagcca ctcggcatag gccaggccca
       61 ggctgatcga gtgcttccgt tcgatttcgt cgggagaata atccgtgagc gcagtgccct
      121 ctttaattga gccatgccgg cgactggagc cggaaacgaa ggcaagcgca tccacgagtg
      181 aggttttgcc actgccaccg tgacccacga aggccacatt gcgaatggcg gttgagccat
      241 agaccttcat ggccgccacc ctccagagct gtagggaaac acaagttggc cctcggactt
      301 gggcttgtca aattgaactg cgtaaacgat gaacgggggt tcgctgggca ccggagtccc
      361 cgttcaccgt ttaccgttca ccgttcacgc agtcacctgt atcccgcggc ctgcaactga
      421 aacagctccg cgtacagccc tccgcgcgcc accagctcct cgtgagtccc ctgctcgacc
      481 agctccccgg cgcgaagcac cagtatccga tcggccatcc gcacagtgga gaaccggtgt
      541 gagatcagga cggccatctt ccctgcagtc agttcggaga accggagaaa tacctggtac
      601 tccgcgcggg cgtcgagcgc tgcggttggc tcgtccaaga tgaggatctg agcgtcccgc
      661 atgtacgcgc gggccagcgc cactttctgc cattcgccgc cggacagctc gaccccaccc
      721 tcgaaccgcc ggcccagcat ctggtcatat cggccggcca ggcgaccggc aaccgagtcg
      781 gccaggctgc gctcggccgc gccctgaatg cgagcctgat cctccagtcg atcgatattt
      841 ccaaccgcga tgttctcccg catgatgaag tcgtagcgaa cgaagtcctg gaagatcacg
      901 ccgacattgc gacgcagact gtcgatgtcg taatcccgga gatcgacgcc gtcgagcaga
      961 atgcgcccct ctgtcgggtc gtaaagccgc acgagcagct tcgcaagcgt ggttttcccc
     1021 gcgccattct cgcctaccaa cgcaatgcgc tccccgggct ccagcgcgaa atcgaggtgg
     1081 cgcactgccc acgtgtccga tcccgggtag tggaaaccga catcttcgaa caccagaccc
     1141 cgctttatcg gaaccgggac cggacgggca ttcggcacgt ttcgcacgcg cggctcgagc
     1201 gcgaaaaagc tgaagaggtc gtccaggtag agactctgct cgaacacctg ggtaagcgaa
     1261 agggagactc gctgtatcag gtcccggctc tgacgaaagg agccggtgag gaatgtgagc
     1321 acgccaatag tgaacaggcc ggcgggaccc cggtggccga tcacggtgag gtagatgatt
     1381 acaacgtagg cgccgtagta ccccaacgtc cctaccgtgg cgaggaggct cgacacgagg
     1441 cttcgacgta cggccagggc tttgttcgcc tcatagaact ctcgactcaa ccggtcgtaa
     1501 cgccccacca ggaaatccga gagcccgaag agcttcacct ccttcgccga gacatcgctc
     1561 gcccccgcat agcgcagata gtcgagcaac cgccgctcgg gcgtccagga gtagagcagc
     1621 gagtatccca acgaggcaaa atgagtctcg cccagcaacg agggcacgac tgcgagcgcc
     1681 aacagcacca gcagccacgg gacgtatgcg accagcgccg ccgagagcgt gaccagggtg
     1741 atcagatcct ggagagtcga gagcagcagg gtaaacagtc cgatgcggtt gaccgtctgt
     1801 cgccgagccc gttcgagctt gtcgtataga tcggcgtctt cgaactgctc caaatccagc
     1861 gtcgctgaat gacgcatgag atcgacactg gtgcgattgg caaacaagtc gcccagcaaa
     1921 ctttcgagca acgcggagag ccgggagagg ccctctccta tcacggcaat ggaaaactcg
     1981 attcccagga gcacgccaag gtgactccag ggaacctgcc gctgctggat ggcggcaacg
     2041 acctggtcca cgatgagctt gccaacccaa agcaccgcaa cagggacgcc ggcccgtacc
     2101 atccggagca gcaggatgcc gacgacatat ttgggctcgg tgctccagac caggcggagc
     2161 agcgttcgga gatgacggat cgcgcggagt cgctctcccc aactcggcgt aggcgcatcc
     2221 ggctttcggc cgccgacgtt ggggcgtaca atcggatggg cgagcggcaa tgacatcaaa
     2281 cgcaacctaa tcgcggggag agccggaccg atggaacctc acctgctcag cgtctggcat
     2341 gatctggtac ggttggggac acccgaaatg gccaacgact gggctcactg gctggaaaag
     2401 ctgatccgcc cagcggttgt atacctcttc ctggtgtttc tgctgcgcgg agccggccgg
     2461 cgaatgctgg cccagctcaa ccccttcgac ttcgtggtcc tgctcacgct ttccaacacg
     2521 gtgcagaacg ccatcatcgg cgaggacagt tcgctgctcg gcggcatcgt gggcgctctc
     2581 gggctcatgg gagtcaacgc cctgcttatc cggcattatt atcgaggccc ctcacaggat
     2641 cggctctctg acaccgatgt ggacatcccg ctcatccatg gtggtgagct cgcagaaagc
     2701 gagctgcgcc gcttccacat caatgcggga gaattggtgg caaaggcaca cgagcgaggc
     2761 ttcgacagcc tgggagaggt cgagagcgcc atgctctacc ccaacggaac cgtctacttc
     2821 cgcgctcgcc atccggatga agcggaacga cgccatcggc agctgatgga acgcctcgat
     2881 cacctgcagc gcgaggtcgc gtcgctgcga attagccact gaacgaccta ttattggcga
     2941 cgatgcccga tacaccgatc cccgagcccc gcacagcgct cgtcaccgga gccaccgacg
     3001 gcatcggacg agccaccgca ttcgcgcttg gtcgcgcagg ctggcaagtc ggctgctgtg
     3061 cccggaccgc cggcaaggtg gaggcgttac tcgatcaact ggccgaggct ggtatccgag
     3121 ccggcggtcg acccgcggat gtgggcgaag agcgagaggc acgaaatgcg gtacgccatg
     3181 tcgtcgacac cctggggccg atcgacgcgc tgatcaacaa cgccgggatc ctgatcgcga
     3241 agccgttcga gcagcttacg ctggccgatt gggatcacac gatggcaacg aacctgcgca
     3301 gcctttacct catgacgcgg gaggtcctcc ccgacatgcg gcgacgacgc aggggcgcca
     3361 tcgtcaacgt cgcgagcctg gccgggcgca acggatttgt gggtggcacc gcctacaccg
     3421 cttcgaagca tgcggtgctt ggcttcagcc gatccttgat gttggaggtg cgcaaggacg
     3481 atgtccgggt gatcgccatc tgccccggct ctgtcgatac gggcctgctt cacgaccagc
     3541 ccctgctcaa gactgatccc acaaggattc tgcgccccga ggacgtcgcc gcggtcattg
     3601 ttcatgccct cgagcttccg gcccgtgcgt tggtgagtga gctggacatc cggcctacca
     3661 atccatgacc ggagtgctgc ccgatctacg ggcggcgttc cgcttccctc ttgacctcag
     3721 gtcagttgac ccgcttccac cccggcatca cgggcatcgg aagcatcact gctcggcgga
     3781 ccagctcgac gtacggcgcc accagatccg ccagctgctg aaccggagcg acatcggctc
     3841 ggctgatcga gccgaatccc cgcgcggcga gcaccagcgc gccaatcgtc tttcccgaaa
     3901 cccgcaacgg cacgatgagc tccgcaccgg ttcgggtttc gctcatgaga tcgggcaggt
     3961 gcccggccaa gacctgcccc agcggagtcc cggaaacagg aacgagtggc aggtccgacc
     4021 aatgcttgtt ctcccccggt tcgatcaaca ccacacgatc cgcctcggtc agccggacgg
     4081 cgagggtaag ccgctcgcag ggcatcatcg agcgagtctc ttcggccaga cgccgggtcg
     4141 cctcggcgaa ttgagcggtc gtagccagca tgtcggcgac gcgggcaaag tgagccggtg
     4201 cgctgcggag cgtgaccagc tgctttcgta acacgtgcag ctgagaggtc agcacgtagc
     4261 cgtcgatctt cggcccgaga atgtggccca gccttacaag catctcgaga tcgccggtgc
     4321 tgaagaagcg gggccgagtg ctgccgacga gcagatggcc cgccaggtgc ccgctcgata
     4381 tcacgcgcac accaatgaca gagcgaagcc gctgcccggg cggaagctca tccacgagaa
     4441 ggtcggccgg ccagcgcgaa tccgccgcga catcggccag gtcgagtgtg ggctcgccgg
     4501 caaacagagc gaacagatcc aactggtgtt tcccgataat cagattggcc tccgcccagg
     4561 gcggcccgtc gggatgagcc ccgagccgat attgttgacc tggtgccggg ccggggatca
     4621 gtacttcgaa gagatcgtgg ggcaccacgc gccccagcac ctgggaggcc agcccgaaga
     4681 agtcgcgggg cgaccgcgcc tcgatccatg cattggcaag ctccgcgccg aggtcgggcg
     4741 cagctgcctc tgcgtctgtt gaccgctgct gggcgattcg tgcaagcatc ggagcgatcg
     4801 cctcaccaat gcgctcgagt agtccgggcg cggcctcgtc gtaccggttg gcttcgagcg
     4861 cggcgatgag cagcagcccg acgtccgttc ggccgaagcg aatcggaagg cactcagccg
     4921 actgataccc cgctcgacgt acgatgctcg ccagctcctc cgcctgctcc ctggaaaccc
     4981 tcggctgcgg gagcgggacc ggaagccggt cctgcgacag agcttccggt ccgatcagca
     5041 cagctccgcc ggcggcagga tacagccaca acccaagcag atcatgaggc aactccaccc
     5101 cgatcgagtc cgccaaggcc tcgtgccaag tcgcaagcac ggcctggtcg ggcgcgccca
     5161 gctccgcccg gaggagcgga atgagaggaa gcggtctgag gtcgggtgct tcgttcatgg
     5221 aaaggccttg agagcaggag atatgcccct gaggccccgg caggtaacta cttgccgctt
     5281 tgacacttga gccagcaacg ccgctcgctc attcacccag gtgttggcga cggttccctc
     5341 agcgcggcgt tttgcacctg accgggggtc agggaacctg cgcggctaac cgatgctcga
     5401 ttagcgcgtt gacatacccc ctccccctat ctatacttcc gccatgaccc aggcaaccct
     5461 ccacatccag gggatgagct gcgcccactg cgtcaacgcc gtccgtcggg ctctctccac
     5521 cctgcgaact gtcgaaatca cgtccctcca gttgggtcgc gctgatctca ccttcgacga
     5581 aaccgtcgtc acacctgatc ggctgactgc cgtggtgagc gatgccggat acatcgcaca
     5641 gtttggaaat gtcggccgct agcgcaacct gcaccatccc ggtcaccggg atgacctgcg
     5701 ctgcctgcag cggaagagtg cagcgatctc tcgagcgagc cgcgggggtg agctcggcga
     5761 cggtgaacct gatgaccgga gaagcaacag tcgcgttcga tccgcaggcg attgcgcccg
     5821 accagctggt gcaagtcatc cgcgacacgg gctacggcgc cagtctgcgg gtctcgccca
     5881 ccgacgcgga agacagtctg gagcgtaccg agcgtgagcg cgaagcggag gtgacgacgc
     5941 tcaagcgaaa gctgtgggtc gccctcctcg ctgccgcctt tctcatcgtg caagggcatc
     6001 cccacacatc ggccgcccgt tggatcgccc tcgctctcac gctgccggtg gtgggttgga
     6061 gtgggcgaca cttctacacc cgggcctgga tggcgctgcg ccatggcggc gcggacatga
     6121 acactctgat cgccgtcgga accgccgccg cctttggatt cagtgtattc atgacggctg
     6181 cggccaagtg ggtggccgcg catggcattg ctctggaggt ctactacgag accgtggcga
     6241 ccatcatcgc cctcgtgctt cttggcaacg tgctggaagc gcgggcgaca agccgaacgt
     6301 cgagcgccgt ccgaaagctg atgggcctga gacctaccac tgcccgggtc atccggggta
     6361 acgccgaagc ggaagtggcg atcggccaag tgcgcgtcgg cgacgaggtc gtcgtccgcc
     6421 cgggcgagcg aatcccggtc gacgggatcg tgatcagagg ttcgagtacg gtgaacgaat
     6481 ccatgctgac tggtgagccc gcgcctgtgg tcaaggagct gggcgcagag gtcgtgggag
     6541 ccaccctcaa ccgacatggc gctctgtatg tccgggccac acggatcgga gccgacaccg
     6601 tcctcgcccg catcattgct ctggtgcgcg gcgctcaggg gcagaaagcc ccgattcagc
     6661 ggctggcgga tcgcatttca gccgtgttcg tgccgatcgt cctcgcaatc gccatcatca
     6721 ccttcgccct ttggtacatg ctcggccccg agccgcgtta cctgcacgcg ctcgtggccg
     6781 cggtcaccgt cgtcatcatc gcgtgcccct gtgccatggg gctcgcaaca ccaaccgcgg
     6841 tcatggtcgc aaccggccgc ggagcggagc tcggcgtgct catcaagggc ggcggtcccc
     6901 tgcaacgtgc tgccgaagtc gatacggtcg tgctcgacaa gaccggcaca gtgacggagg
     6961 ggcgcccgca ggtcgtccag ctcgacctcg ggaatgacgc cctcggcctg cttgggtcgc
     7021 tggagcagct gagcgagcac ccgctcggcg agaccatcgt tggagctgcg cggtcgcgcg
     7081 ggttgatcct gcgtacacca gagtcgttcg gcgtaagacc tggaaaagga gtggtgggaa
     7141 cagtcgacgg ccggaccgtc gtcgccggca atcgggcact ccttgcagag aacggcgtgg
     7201 acgcgtccga tctcgattcg gccgcggtca agctgagcga acaggccgcc actccggtat
     7261 tcgtggcggt ggatggccat ccggcggggc tcgtgggcat tgctgatccg gtcaagccaa
     7321 ccagtgccga agccgtgaag cagctcgacg cattgggact ggacgtggtc atgctcacgg
     7381 gcgatgagtc gcggaccgcc gccgcggtcg cgaggaaggt tggaatcagt cgagtgctgg
     7441 ccgacgtcct cccccagcag aagctgaatg aagtacggcg aattcaggga gacggccggg
     7501 tcgtggcgat ggtgggcgac gggctcaacg acgcgcc
//
LOCUS       DASRJQ010000622         3189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1796552, whole genome shotgun sequence.
ACCESSION   DASRJQ010000622 DASRJQ010000000
VERSION     DASRJQ010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1796552"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VFB89_14115"
     CDS             <1..323
                     /locus_tag="VFB89_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28479.1"
                     /translation="VTTRAAHGSFSYPITPFINGTIHAEYSRSEPLESSSNIPASSIL
                     SAGANLGWRVTNWLSATLAYLYIDRKEDTDTRIGSNPIDINRNIFLQNSTENRVTLTI
                     TATF"
     gene            583..1953
                     /locus_tag="VFB89_14120"
     CDS             583..1953
                     /locus_tag="VFB89_14120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family XrtA/PEP-CTERM system
                     glycosyltransferase"
                     /protein_id="HZM28480.1"
                     /translation="MLRVFRHYIPHLPLILFFGDLVVLVAAFYVPTLTETWIAEGVLI
                     PKLLLVVLLVGFVLNLGGLYDVRLAMGHRELLARLLTCQAVAGLLVAAVAFAIPSLRL
                     GRAAFLQIGAAATIGLIAWRAAWIGPFTHRRIRIRVLVLGTGAIGKVIAGLVETGARP
                     FSIVGFLDDNPNAPETVPEPYALLGKIQDLPALVEETRPDLIVVAQMNRRGNFPAKAL
                     IDCRMRGIQVEDWPTFYEKETGKILVTDLRPSWLIFSDGFVKTPRTEIVKRLVDVALS
                     LTGLVLALPVMALVATAIKMESRGPALFRQPRLGQNGRVFILNKFRSMREDAEKDTGP
                     VWALQQDPRVTRVGAFLRKTRLDELPQLFNVLVGDMSFIGPRPERPEFVYELQKQIPF
                     YMERLSVKPGITGWAQVRYRYGASVEDALEKLQYDLYYIKNLSLFLDLLILINTIQVV
                     LFARGR"
     gene            1999..>3189
                     /locus_tag="VFB89_14125"
     CDS             1999..>3189
                     /locus_tag="VFB89_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28481.1"
                     /translation="MNNVQVLKQIALSVFTRRKKWVLLVSLLGVVLFMPIAYVTSKEP
                     PRHRTVSTILMETRPDRAPLFQEFSPFRPLDVQLAILKSRSLSEAVVESLPRNSVDDL
                     VQNPYSIDYNLEFQNWIRRLRGEEVVVESPQRRALNELRNSRVKFRPVGESGIVEITA
                     EASQPRVAMDIANTYIEVLVSRTRSFNVEDSKTTREFLEQQQAQIGKTLNSAEENMRQ
                     FTNARGGVRIPARHAEAVQRLGQLENTYAEVVSNKNMAQSRLAALKSKVDALPPSSVP
                     VAKPAPTNPAANPSAQRVGRLRAKLQTLETQLLEYQTQYTDEHPRVLTVKRQIADVQK
                     ELGDAVKETTPVPTAGSTVPAPDRSAFAETMAALETSSLSLNAQEEALREQINALKKS
                     LTGLS"
BASE COUNT          583 a         1087 c          967 g          552 t
ORIGIN      
        1 tcgtcacgac tcgcgccgca cacgggagct tcagctaccc gatcacgccg ttcatcaacg
       61 gcaccatcca cgccgagtac tcccggagcg agccgctcga gagcagctca aacattcccg
      121 cgtcgagcat cttgagcgcg ggcgcgaacc tgggctggcg cgtcacgaac tggctctcag
      181 cgacgctcgc ctacctctac atcgatcgta aggaggacac cgacacaagg atcggctcca
      241 atccgataga catcaatcgg aatatcttcc tccagaactc gaccgagaac cgcgtcaccc
      301 taacgattac cgcgacgttc tagccgccct cctcctgctg catcgtcgcc gaaccacgca
      361 aatcatgcgt ggagccctga acggtgtaac tgattgattc caagccaata tttccccccg
      421 agtcgttttg ccacgcagaa tcagcaggat gagtagaaga attccctata caggaaggga
      481 aatgctctct ctgacaggac tcaactagcg taaattcaat aagttacctc tggccggttc
      541 aaaaggcctt ggcacctcga ttgctccctt ttcgcgccgg tcatgctgag agttttccgg
      601 cactacatcc cgcacctgcc gctgatcctc ttcttcgggg atctcgtcgt tctggtcgcc
      661 gccttttacg tccccacgct gaccgagacc tggatcgccg agggggtcct gatcccgaag
      721 ctgctactcg tggtgctgct ggtggggttc gtcctgaacc tcggaggcct gtacgatgtc
      781 cgcctcgcga tgggacaccg ggagctactg gcccgtctgc ttacgtgcca agcggtggcc
      841 ggcctcttgg tggcggccgt ggccttcgcc ataccgagcc tccgcctcgg ccgggcggca
      901 ttcctccaga tcggggcggc tgccacgatc gggttgatcg cctggcgagc cgcctggatc
      961 ggccccttca cccatcgccg catccggatc cgggtgcttg tgctcggcac cggggccatc
     1021 ggcaaggtca tcgcggggct ggtggagacc ggggcccggc cattctccat cgtgggcttc
     1081 ctcgacgaca acccgaacgc cccggagacg gtgcccgagc cctatgccct gctcggcaag
     1141 atccaggacc tgccggctct ggtcgaggag acgcgcccgg acctcatcgt ggtggcccag
     1201 atgaaccgac gcggcaactt tcccgcgaag gcgctgatcg actgccgcat gcgaggcatc
     1261 caggtcgagg actggccgac gttctacgag aaggagacgg gcaagattct ggtcaccgac
     1321 ctccggccga gctggctgat cttctcggac ggcttcgtca agacgccgcg aaccgagatc
     1381 gtcaagcgcc tggtcgacgt ggcgctgtcg ctcaccggtc tggtgctcgc cctgcccgtc
     1441 atggccctcg tggcgaccgc gatcaagatg gagtcccggg gtccggcgct gttccgccag
     1501 ccccggttgg gacagaacgg gcgcgtgttc atcctcaaca agttccggtc gatgcgcgag
     1561 gacgccgaga aggacacggg cccggtgtgg gccctgcagc aggatcctcg tgtcacccgg
     1621 gtgggcgcgt tcctgagaaa gacccgcctg gacgagctgc cgcagttgtt caacgtcctc
     1681 gtcggagaca tgagcttcat cggcccgcgg cccgagcggc ccgagttcgt gtacgagctt
     1741 cagaagcaga tcccgttcta catggagcgg ctctccgtga agcccggcat caccggctgg
     1801 gcccaggtga gataccgata cggtgcgtcc gtggaagatg cgctggagaa gctgcagtac
     1861 gacctctact acatcaagaa cctgtccctg ttcctggacc tcctgatcct gatcaacacc
     1921 atccaggtgg ttctgtttgc acgggggcgc tgaggccatg acggcccgcg ggacaaccgc
     1981 acgaggagat cgacgctgat gaataacgtg caagtcctca agcagattgc cctctcggtc
     2041 ttcacgcgcc gaaagaaatg ggtgctgctg gtgtcactgc tgggcgtggt gcttttcatg
     2101 cccatcgcct atgtgacctc caaggagccg ccccggcatc ggaccgtctc cacgatcctg
     2161 atggagacga ggccggatcg ggccccgctc ttccaggagt tttcgccctt ccggccgctc
     2221 gacgtgcagc tggccatcct caagagccgc agcctctccg aggcggtggt cgaatccctc
     2281 ccccgcaact ccgtcgatga cctcgtgcag aacccgtaca gcatcgacta caacctcgag
     2341 tttcagaact ggatccggcg gcttcgcggt gaggaggtcg tggtcgaaag cccgcagcgg
     2401 cgcgccctga acgagctgcg gaactcccgg gtgaagttcc gcccggtggg cgagtccggg
     2461 atcgtcgaga tcaccgcgga agcctcgcag ccgcgggtcg ccatggacat cgccaacacc
     2521 tacatcgaag tgctggtgtc gcgcacccgg tccttcaacg tcgaggactc caagaccacg
     2581 cgggagttcc tcgagcagca gcaggcccag atcggcaaga ccctcaacag cgccgaagag
     2641 aacatgcgtc agttcaccaa cgcgcgaggc ggcgtccgca tccccgcgcg gcacgccgag
     2701 gcggtgcagc gcctgggtca gctcgagaac acgtacgccg aggtcgtgtc caacaagaac
     2761 atggcgcaga gccgcctcgc tgcgctcaag tcgaaggtgg atgccctgcc cccgtcgtcg
     2821 gtgccggtgg ccaagcctgc ccccacgaat cctgccgcga acccctccgc tcagcgcgtg
     2881 gggcgtcttc gcgccaagct acagacgctg gagacccagc tcctcgagta ccagacccag
     2941 tacaccgacg agcacccccg tgtgctcacg gtgaagcgtc agatcgccga cgtgcagaag
     3001 gagctcgggg acgccgtcaa ggagaccacg cccgtcccga ccgccggcag caccgtgccg
     3061 gcgcctgacc gctcggcgtt cgccgagacc atggcggcgc tggagacgtc cagcctgtcg
     3121 ctgaacgccc aggaagaggc cctgcgcgag cagatcaacg ccctcaagaa gagcctcacc
     3181 ggcctgtcg
//
LOCUS       DASRJQ010000623         2175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3770059, whole genome shotgun sequence.
ACCESSION   DASRJQ010000623 DASRJQ010000000
VERSION     DASRJQ010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3770059"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1245
                     /gene="istA"
                     /locus_tag="VFB89_14130"
     CDS             <1..1245
                     /gene="istA"
                     /locus_tag="VFB89_14130"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HZM28482.1"
                     /translation="LLAAQERGVGRPSVVERWRDQLVALLAAEPELPTVEILHRLREQ
                     GYAGGKTALYELTQELRPKQTTPLVRFEGVPGEFSQHDFGQVHVRYLDGTTEKIRFFA
                     SRLKYSRWMDVRLVADEAVESLVRSMLSGFEAWGGVPLVAVFDNPKTVVLDREGGVIH
                     WNETFGQTALDYRFAPELCTPRRGQEKGTVENLVGFVKGNFFKVRRFHDRADLEAQLA
                     GWHIEVNTVRPSRATGVTPAARLGAEHERLRQLSIPPAEYALRFSVMVGPTGLVSFHG
                     YRYSMPPEAIGIPGTLWLYPHRVRIVAGRFEREHPRVPEQGKDSLHPEDRTARLAAVA
                     GKRGRLYLKRQEILDLGPAAEIFLTEIVHRHRFTWQAEVEQLHRALLERGPAALHRAL
                     AAANERELFGADHVLRLLREVA"
     gene            1245..2090
                     /gene="istB"
                     /locus_tag="VFB89_14135"
     CDS             1245..2090
                     /gene="istB"
                     /locus_tag="VFB89_14135"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HZM28483.1"
                     /translation="MHELDLEGMLRRLHLPTVRRLYPECELRAEAEDQSYRDFLATLI
                     AEEVAHRTQTRIERSVRKARFPFLRAIEEFDFAFQTSVRLQLLGSYLGPELVSEGRSL
                     ILCGPSGTGKTHLSVAIAYRAIQNGYEVLFTSAAALVETLSRAAHRGELASTLAHYIH
                     PHVLVIDEVGYLTVGADAANLMFQVVNERYLSRKPMLFTTNKPLAAWGLVLHDPDLAE
                     AIIDRVLERGRLVDLRGPSYRTRHLKAVDHRSPAAGGARISGKQRPEFPEPTGGATGV
                     FRLLS"
BASE COUNT          378 a          725 c          693 g          379 t
ORIGIN      
        1 ctgctggcag cccaagaacg cggggtggga cggcccagcg tggtcgagcg gtggcgggat
       61 caactggtcg ctcttctcgc ggctgagccg gagctgccga ccgtcgagat ccttcaccgg
      121 ttgcgagagc aaggctacgc cgggggcaag accgcgctct acgagctgac ccaggagttg
      181 cgtccgaagc agacgacacc gcttgtacgc ttcgagggcg taccaggcga gttctcccag
      241 catgacttcg gacaggtcca tgtgcgttac ctggacggga ccaccgagaa gatccgcttc
      301 ttcgcgtcgc gcctgaagta ctcgcgatgg atggacgtgc gcctggtcgc cgatgaagcc
      361 gtcgaatcgc tggtgcgcag catgctcagt ggcttcgaag cttggggcgg tgtgccgctg
      421 gtcgccgtgt tcgacaaccc caagaccgtg gtcctggacc gcgagggcgg ggtcatccac
      481 tggaacgaga ccttcggaca gacggcgctg gactaccgct tcgctccgga gctgtgcaca
      541 cctcggcgcg gacaggagaa gggcaccgtc gagaacttgg taggcttcgt gaaggggaac
      601 ttcttcaagg ttcgccggtt ccacgatcgt gccgatctcg aagctcagct cgcgggctgg
      661 cacatcgagg tcaacacggt gcgtccctcc cgagccacgg gcgtcacgcc ggcagcccga
      721 ctcggtgccg aacatgaacg gctgcgccag ctgtcgatcc cgcccgctga gtacgcgctg
      781 cggttctcgg tgatggttgg tccgacgggc ctcgtctcgt tccacggtta tcgctactcg
      841 atgccgcccg aagcgatcgg gatccctggt acgttgtggc tgtaccccca ccgggtgcgg
      901 atcgtggccg gacgcttcga gcgtgagcat ccgcgcgttc ccgaacaagg caaggacagc
      961 ctccatccgg aagatcgaac cgcgcggctg gcggcggtcg cgggcaagcg cgggcggctc
     1021 tacctcaagc gacaggagat cttggacctg ggaccggcgg cggagatctt cctcaccgag
     1081 atcgtccatc gccatcgctt cacttggcag gccgaggtcg agcagcttca ccgtgcactg
     1141 ctcgagcgcg gtccggccgc cctgcaccga gctctggccg cagccaacga gcgcgagctg
     1201 ttcggtgccg atcatgtgct ccgcctactg cgggaggtgg cctgatgcat gagctcgatc
     1261 tggaaggcat gctccggcgc ctgcacctgc cgaccgtgcg ccggctctat ccggagtgcg
     1321 agcttcgtgc ggaagctgag gaccagtcct accgtgactt cctcgccacg ctgatcgccg
     1381 aagaggtggc ccaccgcacc cagactcgca tcgaacgctc cgtacgcaag gcgcgcttcc
     1441 cgttcctgcg cgccatcgaa gagttcgact tcgcgttcca gacatcggtg cgcctgcagc
     1501 tgctcggcag ttacctgggg cccgagctgg tcagcgaagg gcgctcgctg atcctgtgcg
     1561 gcccctccgg aacgggcaag actcacctga gcgtcgccat cgcctaccgt gccatccaga
     1621 acggatacga agtcctgttc accagcgcgg ccgctcttgt cgagacgctc tcgcgcgctg
     1681 cgcaccgagg agagctcgcc agtactctgg cccactacat ccatccacac gtgctcgtga
     1741 tcgatgaggt cggctacctc acggtcggag ccgacgccgc caacctcatg ttccaggtcg
     1801 tcaacgaacg ctatctcagc cgcaaaccca tgctgttcac caccaacaag ccgctcgccg
     1861 cgtgggggct cgtgctgcac gaccccgatc tcgccgaggc catcatcgac cgcgtgctcg
     1921 agcgaggacg cttggtcgat ctacgcggac cgtcgtatcg tacgcgtcac ctgaaagctg
     1981 ttgaccatcg gagccctgcc gcagggggcg ccagaatatc cggaaaacag cggccagagt
     2041 tcccggaacc cacaggaggg gcgacaggcg tcttccgatt gctctcgtag caagaacaaa
     2101 ttcttgcccg caggccggac gagatggtga ataatctgtt tcctctcacc aaccacccgc
     2161 cgtggaggcg ggatt
//
LOCUS       DASRJQ010000624         5282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1404858, whole genome shotgun sequence.
ACCESSION   DASRJQ010000624 DASRJQ010000000
VERSION     DASRJQ010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5282
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404858"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VFB89_14140"
     CDS             complement(<1..578)
                     /locus_tag="VFB89_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28484.1"
                     /translation="MRDRLSTDFERFRRELPRDFADYVRQTYRIDLTSRYLGYRIPHP
                     FGKGSGQLSLNPDQLETDRAAGLAFVVLKTLIAETSSGERSMGAWAIHETRMAVERRT
                     GGNGREGWTVTWKGRGWDRSLEEYLTLVRASRDLTNAGGDGILAVPSVKYNLPRLDEP
                     FIGGEYAHTTAALADAWGDGPLPLEKDFSPTLA"
     gene            complement(556..1449)
                     /locus_tag="VFB89_14145"
     CDS             complement(556..1449)
                     /locus_tag="VFB89_14145"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HZM28485.1"
                     /translation="MGASVADLVERIRGGLLPAVPVPWSNGRLDETALAGYVEWMARH
                     PVAGVGVSVHTGRGRHLTPADRKRVLTAWRNGFGDRVIVAGAWDDASAAEAKDLGADA
                     VLAFPRRNDPVGYHRLLGNSLPVIVFYLYEAAGGVAWPLDTVAELLSLPSVLGIKIAT
                     LDSVMTFQDLAVRVRGQPGKLLVTGEDRFLGYSLLLGAEAALIGMGAALPDLQAELLR
                     TRANGDWSRFIRLSALCDELAQVTFRAPMEGYVRRMLWAAAADGAIPREACEDPWGPA
                     LPAHERDEVEQVVRRARSAFD"
     gene            complement(1465..1791)
                     /locus_tag="VFB89_14150"
     CDS             complement(1465..1791)
                     /locus_tag="VFB89_14150"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZM28486.1"
                     /translation="MSNAERQHYRIQYPVAARPHITIEGRSFEVIDLSEGGVRFRTED
                     TPSFAVADKVSGRVRFQRTEAVEVKGAVVRLGSREVALKLDVAIPLRVIIEEQRYLRE
                     QHRGMA"
     gene            complement(1788..2561)
                     /locus_tag="VFB89_14155"
     CDS             complement(1788..2561)
                     /locus_tag="VFB89_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28487.1"
                     /translation="MRIDVNVFLGAYPFRRVPGTSPDAVVGAMDRARIDRGWVTHLPG
                     LFWRDPAEGNPWLYETTRRQARLQPIPAIHPGLPGWEQVLDEALASSAPAVRCDPTFY
                     GLDPAGPAMRDLVAACGEAGMLLLLAVRLEDGRQRHVNDRAPELPAAAVRALIRSHPT
                     ARLLVTHAHRSFVEEVHFGSTPSEAARIWWDVCWLWGPPEDDLATLLGSVGADRFVFG
                     TGQPLRLPEASVARLDLLDLSGDDRARIEQLNAERAAAR"
     gene            complement(2561..3361)
                     /locus_tag="VFB89_14160"
     CDS             complement(2561..3361)
                     /locus_tag="VFB89_14160"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZM28488.1"
                     /translation="MLIDVHAHFYTDRSARADWRERNASRLAAGERVGITFHVASILG
                     TWGRTSPTYFPSPADLTHANAALRDIEHANPARVRGYVAVNPNFAAHAEREIRNGIKA
                     GMIGVKLAASRRADDTLVDCVATLARELGVPILHHIWQHRRGDVPGQEASDAVELGRL
                     AARHPEVSFILAHIAGGGDWIHSLHAVRNLENIFVDLSGSGVDGGMLEACLAAVGAGR
                     LLWGCDLTIDTGWAKLRYLQHLLSPDDLARVGWKNAAAIFPSGSFPAA"
     gene            complement(3376..4542)
                     /locus_tag="VFB89_14165"
     CDS             complement(3376..4542)
                     /locus_tag="VFB89_14165"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZM28489.1"
                     /translation="MSTGPVAGFTSTGSKRPEALFGADADWTPPGRMVRSEGCRVWDA
                     EGREYLDFIMALGAVALGYGHPAVARSAIDAVGAGVVGPLAPVLEEALAADLRRLMPW
                     MEQMRFLKSGAEACAAAVRLARVATGRELVLGCGYHGWLDWCEGSVGVPAGTRALFGE
                     LPFNDVDGTRRTIAAAGDRLACVIAEPIVVHEPTPEWLSVVRGETERVGALLIIDEIK
                     TACRLELGGGTQRYKLRPDLVVLGKAIANGFPLAVVGGRREIMAGVGRTWISSTLATE
                     MVSLAAARATLEIVQREQVPHRLRATGSRLLDGFRTLEARHPRLLTGVGGIPQMCFLQ
                     FADDTVSRAVARGAADRGLLFKRSAYNFVSLAHTESVVDRALSVLEDVVAQLEA"
     gene            complement(4539..>5282)
                     /locus_tag="VFB89_14170"
     CDS             complement(4539..>5282)
                     /locus_tag="VFB89_14170"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HZM28490.1"
                     /translation="IATAAGQTVRLGGRRAEARIFRPLIERDPPLRQTGTAVRVDSPP
                     ALDGTLDAFDTSAALALDHEDQYRRSEEPYSGPEDFSALASVNWDDDALYLGVEVIKP
                     DIVLRAADAAPLLLDNEPDDIHSDGLQVYVRLEPDGPVYGFLVAPEPEGEALRVRVAA
                     GYAGTPEMVGGAWAPADVGYILTLELRLPGWQSRTYGDHIGFDLLINEMLPERERRSG
                     QLVWSGGSGWVWLRGDRQPPDRLGELELA"
BASE COUNT          974 a         1783 c         1693 g          832 t
ORIGIN      
        1 gccagggtgg gagaaaaatc tttctccagc ggaagggggc cgtctcccca tgcgtccgcc
       61 agggcggccg tggtatgcgc gtactccccg ccgataaacg gctcatccaa ccgtggcaag
      121 ttgtatttga cggagggaac ggcgaggatc ccatcgccgc cggcattggt cagatctcgg
      181 gacgcgcgca ccaaggtcag gtattcctcc agcgatcgat cccaacccct tcccttccac
      241 gttaccgtcc acccctcgcg tccattcccg ccggtccgac gttccacggc catgcgcgtc
      301 tcgtgaatcg cccaggcccc catagagcgc tcgccggagg acgtctccgc gatgagcgtc
      361 ttgagcacca caaatgcgag tcccgccgcg cggtcggtct ccagttgatc ggggttgagg
      421 gacagctggc ccgagccttt gccgaaagga tggggaatcc ggtatccgag atagcgggaa
      481 gtgaggtcga tgcggtaggt ctggcgcacg tagtcggcaa agtcgcgcgg caactcgcgc
      541 cgaaagcgct cgaagtcagt cgaaagccga tcgcgcacgg cgtaccacct gctccacttc
      601 atcgcgctcg tgcgctggaa gcgccgggcc ccacggatct tcgcatgctt cccgcggaat
      661 cgcgccgtcc gcggctgcag cccagagcat tcggcggaca tatccttcca tcggcgcgcg
      721 gaacgtcact tgagccagtt cgtcgcagag tgcggacaat cgaatgaacc ggctccagtc
      781 cccgttcgcc cgagtccgca gcagctcagc ctgaagatcg gggagtgctg cgcccatccc
      841 gatgagggcc gcctcagcgc ccagcagaag cgagtagccg agaaagcgat cttcgcccgt
      901 aacgagcagc ttgccgggtt gaccccgcac gcgcaccgcc aggtcctgga aggtcatgac
      961 tgaatcgagg gtggcgatct tgatgcccaa cacggatgga agggaaagca gctcggcgac
     1021 ggtatcgagc ggccaggcca caccgccagc agcttcgtag agataaaaca cgatcaccgg
     1081 gagagagtta cccaacagcc ggtgatatcc gaccggatcg ttacgccggg gaaacgcgag
     1141 gaccgcatct gcgccgagat cctttgcttc ggccgcgctc gcatcatccc aagccccggc
     1201 cacgatcacc cggtcgccaa atccgttgcg ccaggccgtg agaacccgct tgcgatcggc
     1261 gggggtaaga tggcgtcccc gacctgtgtg cacgctcacc ccgactccag ccaccggatg
     1321 ccgggccatc cattcgacgt agcccgccaa tgctgtctcg tcgaggcgcc cgttgctcca
     1381 gggaaccgga acagcgggca gcaatcctcc gcggatgcgc tcgaccaggt cggctacgga
     1441 tgcgcccacg caggacttct cacgtcatgc cattccacga tgttgctctc gcagatagcg
     1501 ctgctcttca atgatgaccc gaagcggaat ggcgacgtcc agcttgaggg cgacctcgcg
     1561 actcccgagg cgcacgacgg cacctttgac ctcgactgct tcggtgcgct gaaagcgaac
     1621 cctccccgag accttgtccg ccactgcgaa cgatggcgtg tcttcagtcc tgaaccgtac
     1681 accaccctcg gacagatcga tgacttcgaa tgaccggcct tcgattgtga tgtggggccg
     1741 tgcggcgacg ggatactgga ttcgatagtg ctggcgctcc gcgttactca tcgggccgcc
     1801 gcgcgctcgg cgttgagctg ctcgattcgg gcacgatcgt cgccgctcag gtcgagcagg
     1861 tcgagcctgg cgacactcgc ttccggcagc cggagcggct gaccggttcc gaagacgaag
     1921 cgatccgccc cgactgagcc gagcagggtc gccagatcgt cttccggggg tccccagagc
     1981 cagcacacgt cccaccagat cctggctgct tcggacgggg tcgacccgaa gtgcacttcc
     2041 tcgacgaagc tacgatgcgc gtgggtcacc aggagccgcg ccgttggatg agagcggatc
     2101 aaagcacgca cggccgcggc cggaagctcc ggcgctcgat cgttgacgtg ccgctgtcgg
     2161 ccgtcttcca gccggaccgc caggagcagg agcatgccgg cctcgccaca ggccgcaacc
     2221 agatctcgca ttgccggacc cgccgggtcg agaccgtaga aggtcggatc gcagcgtacg
     2281 gcgggcgcgc tgctcgcaag tgcttcatcg aggacttgct cccatcccgg gaggccgggg
     2341 tgaatggccg gaatcggttg gagtctggct tggcggcgag tggtttcgta gagccaggga
     2401 ttcccttcgg ctggatcacg ccagaagagt ccgggaaggt gggtgaccca gcctcggtcg
     2461 atccgggcgc gatccatcgc tccgacaacg gcatccggcg aggtgcccgg cacccgccga
     2521 aaaggatatg cgccgagaaa gacgttgacg tcaatccgca tcaggcggca gggaacgacc
     2581 cggagggaaa aattgcggcg gcgttcttcc aaccaacgcg ggcgagatcg tctgggctga
     2641 gcagatgctg gagatagcga agcttggccc agccggtgtc gatcgtgaga tcacaacccc
     2701 agagcagtcg acccgcacca acggcagcca ggcatgcttc cagcatgccc ccgtccacac
     2761 cgctgcccga gagatcgaca aagatatttt ccaggttgcg caccgcgtgc agcgagtgaa
     2821 tccagtcccc gccgccggcg atgtgcgcga gaatgaagct cacctccgga tggcgcgcgg
     2881 ctaaacgtcc cagctcgacc gcatcggacg cctcctgtcc gggcacgtcg ccgcgccgat
     2941 gctgccagat gtggtgcagg atcggcacgc caagttctcg cgctagagtc gcgacgcagt
     3001 cgaccagcgt atcgtccgcg cgccgactgg ccgcgagctt gacaccgatc atcccggcct
     3061 tgatgccgtt ccggatttcg cgctccgcat gcgccgcgaa gttcggattg acggcaacat
     3121 agccgcgaac acgagccgga ttggcgtgct cgatatctcg cagggccgcg ttggcatggg
     3181 tgagatcggc cggagacgga aagtaggtcg gagaggtccg cccccaggta ccaagaatgg
     3241 acgcgacgtg aaaggtgatg cccacgcgtt cgcctgcggc caggcggctc gcattccgct
     3301 ctcgccagtc ggcgcgagcc gaccggtcgg tgtaaaagtg ggcgtggacg tcgatcagca
     3361 tcgccggaag atacgtcagg cctcgagttg agccaccaca tcttcgagca cactcaaagc
     3421 ccgatcgacc acggactcgg tatgcgcgag cgagacgaag ttgtaggccg accgcttaaa
     3481 gagcaggccg cgatcggcgg cgccgcgagc cacggcgcgg gagacggtat cgtcggcgaa
     3541 ctggagaaag cacatctggg gaatgcctcc caccccggtc agcaatcgcg gatgccgcgc
     3601 ctccaaggtg cggaatccgt ccagcagccg cgagccggtg gcccgaagtc gatggggcac
     3661 ctgttcccgc tgcacgattt cgagagtggc ccgcgccgcc gccaacgaga ccatctcggt
     3721 ggccagggtg gaagaaatcc aggttcggcc aacgcccgcc atgatttccc gccttccgcc
     3781 aactaccgcg agagggaatc cattggcgat tgccttgccc aggaccacga ggtcggggcg
     3841 cagtttatac cgctgagtgc cgccgcccag ctccaggcgg caggcggtct tgatctcatc
     3901 gatgatgagc aacgcgccaa cccgttcggt ttcgccgcga accacgctga gccattcggg
     3961 agtcggctcg tggaccacga tcggctcggc gatcacgcag gccagccgat cacctgcggc
     4021 ggcgatggtc cggcgcgtgc cgtccacgtc gttgaacggc agctcgccga acagggcgcg
     4081 ggtgcccgcc ggcacgccca cacttccctc gcaccagtcg agccatccgt ggtacccgca
     4141 acccagcaca agctcgcgtc cggtcgcaac acgggcgagc cgaacggcag ccgcgcacgc
     4201 ctccgcaccg gatttgagaa accgcatctg ctccatccag ggcatgagtc gccgaagatc
     4261 tgcggccagc gcttcttcga gcaccggtgc gagcgggccg accacgccgg cgccgacagc
     4321 gtcgatagcg ctccgggcaa cggcaggatg cccgtagccg agcgccaccg cgccgagcgc
     4381 catgatgaag tcaaggtatt cgcggccttc cgcgtcccag actctgcagc cttcagaccg
     4441 caccattcgg cccggtggtg tccaatcggc gtcggcgccg aacagggcct ccggccgctt
     4501 gctgccggtg gacgtgaagc cggcgacagg cccggtgctc acgcgagctc gagctccccc
     4561 agtcgatcgg gcggctggcg atcgccgcgc aaccagaccc agccactgcc tccgctccac
     4621 accagctgcc ctgatcgtcg ctcgcgctcc ggcagcatct cgttgatcag gagatcgaat
     4681 ccgatatgat cgccgtaagt gcgagactgc cagcccggaa ggcgcagctc gagggtcagg
     4741 atgtatccga catctgcggg ggcccaggcg ccaccgacca tctccggagt gccggcgtat
     4801 cccgccgcca cgcgtactcg caacgcctcg ccctccggct ccggggccac caggaatcca
     4861 tataccgggc catcaggctc gaggcggacg tagacctgga gcccatccga gtggatgtcg
     4921 tcgggctcgt tgtcgagcag aagcggcgcg gcatcggctg cgcggagaac aatgtcgggc
     4981 ttgatgacct ccaccccgag atacaacgcg tcgtcgtccc agttcacgga ggccagagct
     5041 gaaaagtctt ccggtccgga gtacggctcc tcgctccggc ggtattgatc ctcgtgatcg
     5101 agcgctaatg ccgccgaggt gtcgaatgcg tcgagggtac catcgagggc aggcggtgaa
     5161 tcgacccgga cggcggtacc ggtttggcgc agtggcggat cgcgctcgat caggggccgg
     5221 aaaatccgcg cctctgctcg ccgcccaccc aatcgcacgg tttggcccgc tgcggtcgca
     5281 at
//
LOCUS       DASRJQ010000625         2984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_955396, whole genome shotgun sequence.
ACCESSION   DASRJQ010000625 DASRJQ010000000
VERSION     DASRJQ010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955396"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /locus_tag="VFB89_14175"
     CDS             <1..1285
                     /locus_tag="VFB89_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28491.1"
                     /translation="SSTESRTEPRTVYLPPTNGMAHRSSLSPDGKQLLVVEMGSTGEQ
                     GGYGWLPCRVTPFDGSSSGKPVGPAPAQCTDAAWSPDGKWMYFSANTGGGFHIWRQRF
                     PDGTPAQVTFGATEEEGIHFAPDGRSFVTSIGSRQSTVWIHDASGDRQLTSEGFAFFP
                     TVSPDAKKLYYLVQAGGARNFVTGRLWVADLESGQHQRLLPDFQLQHYTISADGQRVV
                     FVALDGAGRTPVWLSVLNGRTAPRRLTTMDARAAFFGAPGEVVFAGEGDFVYRVKDDG
                     SELQKIIPAPMLHLFGVSPDGRWVPAAEGAIPDKRNELVVYPTGGGLPTLICKCYPPQ
                     PIDIGPRPPYLSWTPNGRFLYLTFIASTYAIPLQPGQLLPPIPASGFQSKEAVAALPG
                     ARLISDERVFPGPNPSIYAFMKVAAQRNIYRVPVP"
     gene            1685..>2984
                     /locus_tag="VFB89_14180"
     CDS             1685..>2984
                     /locus_tag="VFB89_14180"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZM28492.1"
                     /translation="MELVDAFLARIAAINPRLNCVVQVNHEAARAAAKLADQGVARRQ
                     RLGPLHGVPITIKDSFDTAGMVSTAGTSGRRDFVPTRDATAVQRLRRAGAILLGKTNC
                     PELTLSYETDNLVYGRTSNPYDVTRTCGGSSGGAAATLAACGSALDLGSDTGGSIRVP
                     SHHCGTAGLKPTSGRVPRTGHIIGAQGVFQSFTHVGPMARYVEDLALVLPLLAGTDFT
                     DAAIVTSPLQPPGAVNLRGLRVAVYTDNDLFAPTPATVATVGNAAAVLATAGCHVTEA
                     IPPAFKATYSIGNRLWRAAGSTTVQQLLQTAGTTEVSPPLQPWLTPHEPIVTADLIRL
                     LEDLDRARGLMLSFLEGFDAILCPACAFAAPTHGATLRDDLDAAYSYSEIYNYVGWPA
                     VVVRAGASAEGLPIGIQIVARPWREDVALSVAARVERELGG"
BASE COUNT          522 a          965 c          925 g          572 t
ORIGIN      
        1 ttcctcgacg gagagccgca ccgagcctcg cacggtctac ctgccgccca caaacggcat
       61 ggcccaccgc tccagtctct cgcccgatgg gaagcagctg cttgtggttg agatgggcag
      121 caccggagaa cagggaggat acggctggct gccctgtcgg gtgacaccct ttgatgggag
      181 ctcttccgga aaacccgtcg gcccggcacc ggcgcagtgt accgacgccg cctggtcccc
      241 cgacggaaag tggatgtact tctccgccaa taccggcggg ggattccaca tatggcggca
      301 gcgtttcccg gacgggacac ccgcgcaggt gaccttcggc gccaccgagg aagaagggat
      361 tcatttcgct cccgacgggc gttccttcgt cacctccatt ggaagccggc aaagcaccgt
      421 atggatccac gatgccagcg gcgatcgcca gctcacttcg gaaggcttcg cgtttttccc
      481 aaccgtctca cccgatgcca agaaactgta ttacctggtg caggccggag gagcgcggaa
      541 cttcgtgacc ggacgcctct gggtcgcgga cctggagtcc ggtcagcacc agcggctgct
      601 gccggatttt cagttgcagc actacacgat ctctgccgac ggccagcgcg tggtgtttgt
      661 cgcgttggac ggtgcggggc gtacgccggt gtggctgtcg gtactgaatg gccggaccgc
      721 gccgcggcgg ctcaccacga tggatgcgcg ggcggcattc ttcggcgcgc ccggcgaggt
      781 ggtgttcgcg ggcgaaggag acttcgtgta tcgcgtcaaa gacgatggca gtgaactaca
      841 gaagatcatc ccagcaccaa tgctccacct gttcggcgtc tcgccggacg ggcgatgggt
      901 gccagcggcg gagggtgcca ttcccgacaa gagaaacgaa ctggtggtgt atccgactgg
      961 aggtggtctt cccaccctga tctgcaagtg ctacccgccc cagcccatcg acattggccc
     1021 gaggcctccc tacttgagct ggacgccgaa cgggagattc ttgtatctga cgttcatagc
     1081 ctcaacgtac gctattcctc tgcagccggg ccagctgttg ccgcccatac cggcctcagg
     1141 atttcaatcg aaggaagctg tggccgcgct gccgggagcg cgcctgatct ccgacgagag
     1201 agtgtttccc ggtcccaatc catccatcta tgcattcatg aaagtggctg cgcagcgcaa
     1261 catttatcgt gtgccagtgc cgtgacccgg cgcccactcc agggttcatg agatgcgtag
     1321 gtcgccaagc ggtacgttcc ggcgcggcat aaccagcgct tgctgctgac caggccctaa
     1381 gcgcttcggc cggcgcgaag tatgttccaa ttgtgggttc gcgccggcct ccgcagggcc
     1441 tggcagcaga agcgcgggtc gttaggcaac cgccgacccc cacgatgcct cgcattccaa
     1501 gctggagcag aggtccatga tctctcgtcg gtcctttgta gcgtccgcaa gttgggccgg
     1561 agcggccgct gctttaccac cgatcagacc ttccggcgag ctcgaggttt ctccgctcga
     1621 tgatttgacg agcagttccg cgacgcgcct cgctgcggcc attcgtggca agaaggtgtc
     1681 ctctgtggaa ctcgtggatg cgttccttgc gcggattgca gcgatcaatc cacgactcaa
     1741 ctgcgtggtt caagtcaacc acgaagcggc gcgtgctgcc gccaagcttg ccgatcaggg
     1801 agtcgcacgg cggcagcgtc tcggccccct tcatggagtt ccgataacca tcaaggattc
     1861 gttcgacact gcgggcatgg tgtcaacggc tgggacgagc ggtcgaaggg acttcgtacc
     1921 cacacgcgat gccaccgcgg tgcaacgcct gcgccgggcc ggggccatcc ttctgggtaa
     1981 gacgaactgt cccgagctca cgctttcata tgagacggac aatctcgtct acggtcgcac
     2041 cagcaacccc tacgacgtga cgcgtacttg cggcggtagc tccggcggcg ccgctgccac
     2101 gctcgccgcc tgcggatccg ccttggatct tggaagtgat acggggggca gcattcgcgt
     2161 gccaagtcat cattgcggaa cggctggcct caagcctacc tcgggtcgag tccctcgaac
     2221 gggtcacatc attggtgcgc agggcgtttt tcagtccttc acgcatgttg gccccatggc
     2281 gagatacgtc gaagacttgg cactggtcct accgcttctg gcagggaccg acttcactga
     2341 tgcagcgatt gtcacgagcc cgcttcagcc gcctggcgca gtgaatcttc gtggcctccg
     2401 ggtggctgtc tacactgaca acgacctctt cgccccgacc cccgcaaccg tcgcaacggt
     2461 aggcaacgcc gcggccgttc tcgcgacggc cggctgtcac gtgacggagg caattccgcc
     2521 tgcgttcaag gccacctact ccatcggcaa ccgtctgtgg agggcggctg gaagcaccac
     2581 cgtccaacaa ttgctgcaga ccgcgggcac aacggaagtc tcaccgcccc tgcagccctg
     2641 gctcacgccg cacgagccga tcgtcacggc ggacctgatc cgtctcctcg aagatctgga
     2701 ccgtgcgcgc ggtctcatgc tctcgttcct agaaggattt gacgccatcc tctgtcccgc
     2761 ctgtgcattt gccgcaccga cccacggagc cacacttcgg gacgatctgg atgcagcgta
     2821 cagttacagc gagatctaca actacgttgg ctggccggcc gttgtggtcc gcgccggcgc
     2881 atccgcggaa ggccttccga tcggaataca gattgttgcc cgtccttgga gggaggacgt
     2941 ggcattgtct gtcgccgcac gggtggagcg cgagttgggt gggt
//
LOCUS       DASRJQ010000626         2770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3969958, whole genome shotgun sequence.
ACCESSION   DASRJQ010000626 DASRJQ010000000
VERSION     DASRJQ010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3969958"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /gene="aspA"
                     /locus_tag="VFB89_14185"
     CDS             <1..277
                     /gene="aspA"
                     /locus_tag="VFB89_14185"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF021900.4"
                     /note="catalyzes the formation of fumarate from aspartate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HZM28493.1"
                     /translation="AERCVAGIEADEAQCAYWLERSPALVTALAPEIGYAEAAKLAKE
                     ALASGLTVKELVLRKRILEGAKLEEVLDLRAMTEIGVPGGKGVPAGG"
     gene            297..755
                     /locus_tag="VFB89_14190"
     CDS             297..755
                     /locus_tag="VFB89_14190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HZM28494.1"
                     /translation="MRVTTWTEYSLIISLHLARRGRTAAEPVAARELADAERLPADYV
                     EQILLRLRRAGLVESVRGAKGGYYLARDPSNISVRDVMTASEHQTFEINCETHPVDAE
                     RCHPANGCSIRPVWLALQQRVDELLASISLADLMKQEAEVQELVTITSSG"
     gene            852..1994
                     /gene="moeB"
                     /locus_tag="VFB89_14195"
     CDS             852..1994
                     /gene="moeB"
                     /locus_tag="VFB89_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_257790189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HZM28495.1"
                     /translation="MSTSLSTTELLRYARHLTLPGVGREGQMRLRSARVLVVGAGGLG
                     SPAALYLAAAGIGTLGLVDFDRVDVSNLQRQILHGTGSVGRPKVESAVERLADLNPEV
                     RVVPIEERLAAANAQDIIGQFDLMVDGTDNFPTRYLVNDVCVWLGKPLVYGSIFRFEG
                     QASVFHAAQGPCYRCLYAEPPPPNLVPSCAEGGVLGVLPGVIGSLQALEAIKLVLGAG
                     ESLIGRLLVFDALGLSFRQLALRKDPDCPVCGPRPTITEPIDYEAFCGIGGQDAPGND
                     EISAAQLSELLERGGAPVHVLDVREPWEWEIARLPQASLVPLGELSERLGELDPRGEI
                     ITLCHHGARSAQAGALLRAAGFSRVRSLSGGIDRWAQEIEPGMARY"
     gene            complement(2090..2173)
                     /locus_tag="VFB89_14200"
     tRNA            complement(2090..2173)
                     /locus_tag="VFB89_14200"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2137..2139),aa:Leu,seq:cag)
     gene            2270..2752
                     /gene="smpB"
                     /locus_tag="VFB89_14205"
     CDS             2270..2752
                     /gene="smpB"
                     /locus_tag="VFB89_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HZM28496.1"
                     /translation="MAPKLPPADDRQSVARNPKATHDYHVLETWEAGLVLTGTEVKSL
                     RNGKASIKEAYARVSRGEVFLEGMNITPYEQGNRYNHDPVRSRKLLLHRREIDRMIGA
                     VERQGLTLVPLELYFKKGRAKVALALGKGKKQHDKREDLKRRIAERETARAVSFRGRR
                     "
BASE COUNT          500 a          866 c          921 g          483 t
ORIGIN      
        1 ggccgagagg tgcgttgccg ggatcgaagc cgacgaggct cagtgcgcgt attggctcga
       61 gcggagcccg gcgctggtca cggccctggc gccggagatc ggatacgctg aagctgccaa
      121 actcgcgaag gaagcattgg cgagcggtct aacggtgaaa gagctcgtat tgcgcaagcg
      181 catcctggag ggggcgaagc tggaggaggt ccttgacctc cgtgccatga ccgaaatcgg
      241 cgtgcctgga gggaaggggg taccggccgg ggggtgatct tgcgatattg ggggacatgc
      301 gggtcacaac ctggacggaa tacagcctga tcatctcgct tcacctggcg cgacgcggac
      361 gcacagccgc ggagccggtg gctgcccgcg agttggccga cgccgaacgc ctgcccgccg
      421 attacgtcga gcagatcctc ctccgtcttc gccgcgctgg cctggtcgag agcgtccgcg
      481 gcgccaaggg cggctactat ctcgcccgcg atccctccaa catctccgtt cgggacgtca
      541 tgaccgcgtc ggagcaccag acgttcgaga taaactgcga gacccacccc gttgatgctg
      601 agcgctgcca tccggccaac ggctgcagca tccgtccggt gtggctcgcg ctgcagcagc
      661 gggtcgatga gctgctcgcc agcatctccc tcgccgacct gatgaagcag gaggccgaag
      721 tgcaggagct ggtcaccatc acgagcagcg gatgaacggc atcgaccgga cggcacggcg
      781 gggctccgat gatcgtcgga gccccgtttt gttttgctgc tctcctgcga tacccctcta
      841 tctttcccca catgagcacg tcgctatcga ccaccgagct gcttcgctac gcccgtcacc
      901 tgacactgcc gggcgtcggg cgggaaggcc agatgcggct tcggtcggcg cgtgtccttg
      961 tggtcggcgc gggaggtctg ggctctcctg ccgcactcta cctcgccgct gcgggcatcg
     1021 ggacgctggg gctggtggac ttcgatcggg tcgacgtaag caacctgcag cgtcagatcc
     1081 tccacggaac cggatcagtc ggccggccga aggtggagtc ggccgtcgag cgcctggccg
     1141 atctgaatcc cgaggttcgg gtagtgccta tcgaggagcg acttgctgct gcgaacgcac
     1201 aagacatcat cggtcagttc gatctcatgg tggatggtac cgacaacttc ccgacccgat
     1261 atctcgtcaa cgacgtctgc gtctggctgg gaaagccgct ggtgtacggc agcattttcc
     1321 gcttcgaggg acaggcgtcg gtcttccatg cagcacaggg accatgctac cgctgcctct
     1381 atgccgaacc gcctccaccc aacctggtgc ccagttgcgc cgaggggggg gtgctcggcg
     1441 tcctgccggg ggtcattggc agcctacagg ctctcgaagc aatcaagctc gtactcggag
     1501 ccggcgaatc gttgatcggg cgattgctgg tgttcgacgc gctcggcctc agtttccggc
     1561 agctcgcact acggaaggat ccggattgcc cggtctgcgg cccgcgaccc accataaccg
     1621 aaccgatcga ctacgaggcg ttctgcggga ttggcgggca ggacgcaccg ggcaacgatg
     1681 aaatttcggc tgcgcagtta tccgagctgc tcgaaagggg aggggcgccg gttcatgtgc
     1741 tcgacgttcg ggagccatgg gaatgggaga tcgcgcgact tcctcaagcg tcgctggttc
     1801 ccctgggcga gctgtccgag cgactcgggg aactcgatcc acgcggtgag attatcacgc
     1861 tctgccacca cggcgcgcga tcggcgcagg cgggcgcgct tctccgggcg gcggggtttt
     1921 cccgagttcg gagcttatcc ggaggaatcg atcggtgggc gcaggaaatt gaaccgggga
     1981 tggcgagata ctgaattgcg ggaacggtca agggtgaacg gtaaacgggg ggcgcccttg
     2041 catcggcact ccccgttcac cgtttactgt gcaccgttca ccgccgtcat gccgaagggg
     2101 ggactcgaac ccccacgggc ttgcgcccac tgcgccctga acgcagcgcg tctaccaatt
     2161 ccaccacttc ggcgagggcg caaacgtagc gcccacgcca ccttggcgtc aagcagccgg
     2221 ttgacccaca agctgctgct ggcctatcat ttgcaaaaaa ttttgacccg tggcacccaa
     2281 gcttccgccg gctgacgatc gtcagtcagt cgcccgaaat cccaaagcaa cccatgacta
     2341 ccatgtcctc gagacctggg aggccggcct agtgctcacc ggcacggaag tgaagtcgct
     2401 tcggaatgga aaggcatcca tcaaagaggc ctacgcccgg gtgtcgcgcg gcgaggtctt
     2461 cctcgagggc atgaacatca cgccgtacga gcagggaaac cggtacaacc acgatccggt
     2521 acgatcccgc aaattgctcc tgcaccgtcg cgagatcgat cgcatgatcg gagccgtcga
     2581 gcgccagggg ctgacgctgg tgccgctcga gctctacttc aagaaggggc gggcgaaggt
     2641 ggcgctggcg ctggggaagg gcaagaaaca gcacgacaag cgtgaagact tgaagcggcg
     2701 catcgccgag cgggagactg ctcgggcggt ctccttccgg gggcggcgtt gatcgccgcg
     2761 ctagtcctcg
//
LOCUS       DASRJQ010000627         2016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1581828, whole genome shotgun sequence.
ACCESSION   DASRJQ010000627 DASRJQ010000000
VERSION     DASRJQ010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1581828"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1310
                     /locus_tag="VFB89_14210"
     CDS             165..1310
                     /locus_tag="VFB89_14210"
                     /inference="COORDINATES: protein motif:HMM:TIGR04390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HZM28497.1"
                     /translation="MPLLALALLSAPGRGMLLAQATAQDNRALQRRAESLRGEQRLTE
                     ARDVYRTLVDRDPHDYENRFWLAKLEGWTGHLASAESLYVDLLAERPEDYDSRIGLAD
                     VLIWSGRYEAAQAHLERLDRAYPGNAEVLARLDRVRAIRHEPPRARWQMGVEYYGDQL
                     TGAAPSNGGTASIAARPSDALRWSAAATVQQKFSQTEARAGGELGHRLGRSFELHGAA
                     YVAPSAEVLPRQTYGFGLSTKLGAGLVLYADYTFLDFLDAQVHQISPRLELYAGPHWF
                     FSGRYGYASTRFDGLTDAVGNHAGAVSVGYFYGVSNSIQVFGGAGGEAFALPSRDAIG
                     GFSAHTVGIGWRQFLTPWLGLTASYSHQNRSDGAKQDSYGLGLVRRW"
     gene            1304..>2016
                     /locus_tag="VFB89_14215"
     CDS             1304..>2016
                     /locus_tag="VFB89_14215"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZM28498.1"
                     /translation="MVTAAPGWEICLIGLLLGAAVLLIALLFFLNHFHTYRAHQVLEL
                     ERMLEETVEEWAHDDLMPDESQRLARLRGGDRDVLVQVCLQMAPSLDQRALERVRAGL
                     KRWGLLDRELANLHHPSPARRADACLVLGRMGWRSAIPALIQALSDPHPLVRRRAIAA
                     LGDLHAVEALRPIVRTLEATGSWGDMLAIMALSRLGAESIPHVTALLQESTSPAMTKG
                     LLQMTAQLGRAADPALVRDL"
BASE COUNT          302 a          690 c          657 g          367 t
ORIGIN      
        1 accatgtctc tttaaagaac ttacgtgccc tgtcccgggg cacgccgatt gcgcttcgtt
       61 cctaggccgt ttcgcccagc cgagaccggt ctcacccaac accctcggat gtctgccgcc
      121 aggcgggcca cgtgagaagc cgaatggtga ttcatcggtt tgttatgcca cttcttgcgc
      181 ttgctttgct ttcggccccg ggccggggga tgctcctggc ccaggcgacg gctcaggaca
      241 accgcgccct gcagcgccgc gccgaatcat tgcgtggcga gcaacgcctg accgaggcac
      301 gcgacgtcta tcgcaccctg gtcgatcgtg atccccacga ttacgagaac cgtttctggc
      361 tggccaaact cgagggttgg accggacacc tggcctccgc cgagtcgctg tatgtcgacc
      421 tgcttgcgga gcggccggag gattatgaca gccggatcgg gctggccgat gtgctgatct
      481 ggtccggtcg ctacgaagcc gcccaggcac atctcgagcg gctcgaccgc gcctatcctg
      541 gcaacgccga ggtgctcgct cgactggatc gggttcgcgc catccggcat gagcctcccc
      601 gggcccggtg gcagatggga gtcgagtact acggcgacca gctcactggc gccgccccca
      661 gcaacggcgg cactgcgtcg atcgcggcgc gacccagcga tgctctgcgc tggagtgcgg
      721 cggcgaccgt gcagcagaag ttctctcaga ccgaggcgcg cgccggcggc gagctgggcc
      781 atcgcctcgg gcggagcttc gagcttcacg gcgctgcgta cgtcgcaccg agtgcggagg
      841 tcctgccgcg ccagacctac ggattcggcc tcagcaccaa gctcggcgca ggactcgtcc
      901 tctacgccga ctacaccttc ctcgatttcc tcgacgcgca ggttcatcag atcagccctc
      961 gcctcgagct ctatgccgga ccgcactggt tcttctccgg tcgatacggg tacgccagca
     1021 ccaggttcga cggcctgacc gatgcggttg gcaaccacgc gggcgccgta tcggtcggct
     1081 atttctacgg cgtaagtaat tccatccagg tattcggcgg agccggcggc gaagcattcg
     1141 cgctgccctc gcgcgacgcg atcggcgggt tcagtgccca caccgtgggg atcggctggc
     1201 ggcagttcct gacgccgtgg ctcggcctca cggcctcgta ctcgcatcag aaccgctccg
     1261 acggagcgaa gcaggattcc tacggcctcg gtttggtgcg gcgatggtga ccgccgctcc
     1321 gggctgggag atctgcttga tcgggctgct gctcggcgcg gcggtgctgc tcatcgccct
     1381 tctcttcttc ctcaatcatt tccataccta tcgcgcgcac caggtcctcg agctcgaaag
     1441 gatgctggag gagaccgtgg aggagtgggc ccacgacgac ctgatgcccg acgagagcca
     1501 gcgcctcgct cggctccggg gcggcgatag ggatgtcctc gtgcaagtct gtctgcaaat
     1561 ggctccttcg ctcgaccagc gcgcgctcga acgggttcgc gcggggctca agcggtgggg
     1621 cctgctcgat cgcgagctgg ccaacctgca tcacccgagc ccggctcggc gcgccgacgc
     1681 ctgtctggtg ctcggtcgca tgggttggcg gtccgcgatt ccagcactga tccaggctct
     1741 gtccgatcct catccactgg tccggcgacg cgcaatcgcg gccctgggcg acctccacgc
     1801 ggtcgaggcg ctgcgaccga tcgttcggac cctcgaggcg accggcagct ggggcgacat
     1861 gctcgcgatc atggccttga gccggctggg tgcggagagc atcccgcacg tcacggcatt
     1921 gctccaggag tcgacctcgc cggccatgac caagggactg ctccagatga ccgcgcagct
     1981 gggtcgagcg gctgatccgg ccctggtgcg tgatct
//
LOCUS       DASRJQ010000628         2016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1233241, whole genome shotgun sequence.
ACCESSION   DASRJQ010000628 DASRJQ010000000
VERSION     DASRJQ010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233241"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="VFB89_14220"
     CDS             <1..1146
                     /locus_tag="VFB89_14220"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HZM28499.1"
                     /translation="SVTQDSLDTFSVSVPYGKFDEGPAAQCVAHRYGTNHHATTIDGN
                     LMELLPAAVYHLDEPSDPLSVCLYELSRFARRYVKVALGGDGGDELFGGYDRYYGTLY
                     ARYYAALPAGFRKQVVQRILRLAPDGFWYKSLTHQIRWLDELAQVSGGRRYARSLGYA
                     YFTPSRRETLYTHQFRDAVAALDPEAALIRWHEEGHVKEAVDRMLLADSMIRLPNHSV
                     MILDRMSMAHGLEARSPFLDHELAQFAATLSPRFKIRGRHRRYLQMRLAERYIPAEVL
                     SRPKQGFSSALPYLMDRQFGLLFRGFFRDARLVQAGICQPATIRQMLEAHLAGRSDHG
                     NRLWLLLNAELWYRVHIEGQRVGDLTDQTAELVRLATRAASPAPVGA"
     gene            complement(1047..>2016)
                     /locus_tag="VFB89_14225"
     CDS             complement(1047..>2016)
                     /locus_tag="VFB89_14225"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HZM28500.1"
                     /translation="FHTKFTVPLRGDVPAVMALHGGSWYVEPSFYPWWDNLYIRTMMP
                     LYCRRAIHMISNSECTTEDYVNLIGADRRKITTVPLAAAPCFRPIIDAAELARVRRRY
                     HLPDGYTLSVSVLDVRKNVPRLLRAFHLARRSHPCHLVLVGADRSAVAARFPEIAELL
                     DDHVICTGWVPQHDLPAIYSMARIFFFPSIYEEFGIPNCEALACGTPILTANRAGPPE
                     IVGDAGLLVDPLDVEAMRAGLVRLLEDEPLRQDLSRRALLRSRLYTWRETGRRTLEVL
                     RAVAGQAGKMRVEGQAPTGAGEAALVASLTSSAVWSVRSPTRCPSM"
BASE COUNT          384 a          634 c          635 g          363 t
ORIGIN      
        1 agcgtcacac aagactcgct ggatacgttc tccgtgagcg tgccctacgg caagttcgac
       61 gaggggccgg cagcccaatg cgtcgcccat cggtatggaa cgaatcacca cgcaaccacg
      121 atcgacggca acctgatgga gcttttgccc gccgcggtct atcaccttga cgagccatcc
      181 gatccgctga gcgtctgcct gtatgagctg tcacgctttg cacggcgata cgtaaaggtc
      241 gcattgggcg gcgatggagg cgacgaactg ttcggcgggt acgaccgcta ctacggcact
      301 ctctacgctc gatattatgc cgctctgccg gccggtttcc ggaagcaagt cgtccagcgc
      361 atcctcaggc tcgcacccga cgggttctgg tacaagagcc tgacccatca aatccggtgg
      421 ctcgacgagc tcgctcaggt gagcggcggt cgccgttacg cacgaagcct tggctacgcg
      481 tatttcacac cgagccggcg cgaaacgctc tacacgcatc agttccggga tgctgtcgcc
      541 gctcttgacc ccgaggcggc cctcatccgc tggcacgagg agggacacgt taaggaggca
      601 gtggatcgga tgctgctggc ggacagcatg atcaggctgc ccaaccactc ggtgatgatc
      661 ctggatcgaa tgagcatggc tcatgggctt gaagcacgct ccccatttct ggaccatgag
      721 ctggcacagt tcgcggccac gttgtcgccc cgcttcaaga tccggggccg ccatcgccgc
      781 tacctgcaga tgcgactcgc cgagcgatac atcccggccg aggtgctcag ccggccaaag
      841 cagggctttt cgtcagcgtt gccctatctg atggaccggc agttcggcct gctgttccgc
      901 ggcttctttc gggatgcccg gctggtacag gcggggatct gccaacccgc cacaatcagg
      961 cagatgctcg aagcccacct ggcagggcga agcgaccatg gtaaccgcct gtggctactg
     1021 ctgaatgccg agctctggta tcgggttcac attgaaggac agcgggtcgg ggacctcacc
     1081 gaccagacgg ctgagctggt caggcttgct acgagggcgg cttctcctgc accagtcggc
     1141 gcctgacctt cgacgcgcat ttttcccgct tgcccagcca cggcgcgtaa tacctccagc
     1201 gtacgtcgcc cagtctcgcg ccaggtgtag agcctgctcc gcaggagggc gcgccgcgag
     1261 aggtcctgcc gcaacggctc gtcctccagc agccgtacca gccctgcccg cattgcttcc
     1321 acatcgagcg gatccaccaa caagccggcg tcgcccacga tttccggcgg tccagcccga
     1381 ttcgctgtca ggatgggtgt gccgcaggcc agggcttcgc aattgggaat tccaaattcc
     1441 tcgtagatgc tggggaagaa gaaaatccga gccatcgagt agatagcggg gagatcgtgc
     1501 tggggtaccc agccggtgca aatcacgtga tcgtcgagga gctcagcgat ctcgggaaat
     1561 cgcgcggcga cggccgaccg gtcggccccg acgagcacca ggtggcaggg atgagaccgg
     1621 cgtgctagat ggaacgcgcg gagaaggcgg gggacgttct tgcgcacgtc gagcaccgag
     1681 acagacagtg tgtacccatc cggcaggtga tagcggcgcc ggactcgcgc cagctcggct
     1741 gcgtcgatga ttggtcggaa gcagggtgca gctgccagcg gcaccgtggt gatcttgcgg
     1801 cgatcggccc cgatcaggtt gacatagtcc tccgtcgtgc attccgaatt cgagatcatg
     1861 tggatcgcac ggcggcagta caacggcatc atcgtccgga tatacagatt gtcccaccaa
     1921 gggtagaacg agggctccac gtaccaactc ccgccgtgaa gagccatcac cgcggggacg
     1981 tcccctcgta gtgggacagt gaacttggta tggaag
//
LOCUS       DASRJQ010000629         6035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3488616, whole genome shotgun sequence.
ACCESSION   DASRJQ010000629 DASRJQ010000000
VERSION     DASRJQ010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6035
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3488616"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1298)
                     /locus_tag="VFB89_14230"
     CDS             complement(<1..1298)
                     /locus_tag="VFB89_14230"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM28501.1"
                     /translation="MTRTLIIAALLVARPLAAQSTLLGDSEHPVAGSGPDSVLIAAQL
                     ALDRGLPWRASQMLEPIVRDSVRRAPDAILLAANAAARWGGWSQVQRLLASVVWKGTP
                     AAGSADLLASRAAVELGSDSLAAAQARAAANESADPLRRAEAMIVLGRALDRLGQSDS
                     AAVVYDSAASLAPQAGDWLRLRSASLTADSAQRGHRLAPIRLPAALDRFAATEAVARE
                     KAGDLLGAAALYRSMGNPVAALRLQLAAQSDVETRDSVRQALLLLLGQQSPGIVRGAG
                     ALLDSALAPLTSEQELAVARAIGPAGVPSRAVAGFERAFKAELGNAQDRFDYAGALFK
                     LGKYADAAKAYARVPTHDRKLGGAAAYQRARSLLRDGQLEPARKAFKAVAHKFSRDTS
                     AAPPALWLLADLAADDDQDADARKLYRELSKRFPESRFAPG"
     gene            1383..1775
                     /locus_tag="VFB89_14235"
     CDS             1383..1775
                     /locus_tag="VFB89_14235"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HZM28502.1"
                     /translation="MTEEERVRIDKWLWAARFFKTRSLASEAVAGGKVEVNGERAKPA
                     KLVRPGDEVRLRAGPYEYVLVVRGLAERRGSAKVAATLYEERPESRAERERVAGQLRL
                     GAAAWTYEEKGRPTKRDRRALEKFRRRD"
     gene            complement(1791..3047)
                     /gene="icd"
                     /locus_tag="VFB89_14240"
     CDS             complement(1791..3047)
                     /gene="icd"
                     /locus_tag="VFB89_14240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HZM28503.1"
                     /translation="MSILSAPAYKELRTPTDGESIAMRDGKLEVPSHPIIPFIEGDGT
                     GPDIWRAAQQVFDGAVTKAYGSDRRIVWFEVYAGEKAKNRLNNWLPDDTLTAISHHLV
                     AIKGPLTTPVGGGFRSLNVALRQKLDLYACVRPVRYFEGVPSPVKAPQDVNMVIFREN
                     TEDIYAGIEYKARSEDARKLIRFLQEEMGAQTIRFPDSSAIGIKPISEEGSKRLIRAA
                     IDYASREGVASVTLVHKGNIMKFTEGGFRDWGYQLAKEEFGATEVDGGPWCKLPNGVV
                     IKDVIADAFLQQILTRPRDYGVIATMNLNGDYISDALAAQVGGIGIAPGANINYVTGH
                     AVFEATHGTAPRYAGQDKVNPGSLILSGEMMLRHLGWSEAADLIVSSLERTIGQKKVT
                     YDFERLMSGATLVSTSGFARAMLENM"
     gene            3104..4834
                     /gene="aceK"
                     /locus_tag="VFB89_14245"
     CDS             3104..4834
                     /gene="aceK"
                     /locus_tag="VFB89_14245"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF002804.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HZM28504.1"
                     /translation="MNGNVRPEPAAAGIFGEYQLWYTEFAEVTRRARQRFQSRDWHGT
                     QADAAERLALYRLHVETATAYLHQVLDGDIQDRKTWTRLRSLYAGLTEGRDDAELAET
                     FFNSVSRRVLGTIGATSDAEFIEFPASGPLARTGPMLFDTYRGEGTSRDLVSRLLAAC
                     GWDVPYANASHDVELVASVIDNRLCELRWERGPLAIDVLRSPFYRNKGAYLIGRVHGA
                     NELLPLVVALVHGKEGILVDAVLLTQDEASVVFGFSWSYFFVEAPHPRGLITFLQSIM
                     PHKRIDELYTSIGYSKHGKTELYRSLMRHMERPEARFALAEGDEGMVMCVFALPSFNL
                     VFKIIRDRFGAPKNTTRRAVMDRYHFVFQRDRVGRLADAQEFENLELRRRCFPEDLLA
                     YLKSHAGRSVEVKDESVLLRHVYTERRVTPLNLFLLQADAGTARETILDYGNAIKDLA
                     GANIFTGDMLLKNFGVTRNGRVICYDYDELCLLTDCTIRRLPPARTPEDEMAAEPWFH
                     VGEHDVFPEEFAAFLIPGDRLRGTFLGAHSDLLTPDFWTGVQRRLEAGEIFDFFPYKQ
                     ARRLHRDGAG"
     gene            complement(4869..5606)
                     /locus_tag="VFB89_14250"
     CDS             complement(4869..5606)
                     /locus_tag="VFB89_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28505.1"
                     /translation="MQRYWLRIALGAAAIFVLGMGVITLTRNSIGELRALAASTRPIS
                     IPLVIMPFRLDSTQIGTVRRLDILRRSPREVSGIRLTVRLDDSVPAARLPDCRVTLFE
                     PEGFADGSGFRCVGAADSAAQNLKLIGEVAFEPGGVVRALFAPADHADQWRSHDSAEV
                     RAEFAKLQAGHLSDSAVRAVIRADSLGTLLDIGNKSGKGLVHIQTDSHGASLQIRDRS
                     GRKIVQMQADPAGARLSVLADSAPPAR"
     gene            5720..>6035
                     /locus_tag="VFB89_14255"
     CDS             5720..>6035
                     /locus_tag="VFB89_14255"
                     /inference="COORDINATES: protein motif:HMM:NF015691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HZM28506.1"
                     /translation="MPVPVQSIDLNADVGEGFGVWPPLADADLFPLISSASIACGFHA
                     GDPTLMRETAMLASRRGVVVGAHPGYPDLPGFGRRELGASAAEIRDYVTYQVGAMMAC
                     ALA"
BASE COUNT         1094 a         1954 c         1897 g         1090 t
ORIGIN      
        1 ccgggagcaa agcgagactc cgggaagcgc ttgctcagct cgcgatacag cttgcgtgca
       61 tccgcatctt ggtcatcatc cgccgccaga tcagccagca gccagagcgc gggaggagcc
      121 gcggaggtgt cccgagaaaa cttgtgtgct accgccttga acgcctttcg tgccggctcg
      181 agttgtccat cacgcaacag cgatcgcgcg cgctgatacg ccgccgcgcc acccagcttc
      241 ctatcgtgcg tgggaacacg tgcgtacgct tttgccgcat ccgcgtactt gcccagcttg
      301 aacaacgcgc cggcatagtc gaatcgatcc tgcgcattgc cgagctcagc cttgaacgct
      361 cgctcgaatc ccgcaacggc gcgggacgga acaccggccg gaccgatggc cctggcgacg
      421 gcgagctcct gttcggacgt gagcggtgcg agtgcgctgt cgagcaaagc ccccgctccc
      481 cggacgattc caggggactg ctgtcccagc aacagcagga gcgcctgccg gactgaatcg
      541 cgagtctcca catccgattg cgcggccagc tgcaatcgaa gcgcagccac cggattcccc
      601 atgctccgat agagagcagc agcgcccagc aggtcccctg ccttctctcg cgcaacggcc
      661 tcggtggcgg caaaccggtc cagcgccgcg ggcagacgga tgggcgccaa gcggtggcct
      721 cgctgggcac tgtccgccgt tagagaggca ctgcggagtc gaagccaatc ccccgcctgt
      781 ggggccagcg aggccgcact gtcgtacacg acggcagcgc tgtcgctctg tcccaatcga
      841 tcgagtgccc ggcccagcac gatcatcgcc tcggctcgac gaagcggatc ggccgattca
      901 tttgcggccg cgcgggcttg cgccgctgcg agagaatccg atcccagctc tacggcggca
      961 cgggaggcca gcaggtcggc ggatccggcg gccggggttc ctttccagac cacgctcgcc
     1021 agaagccgct gcacttgaga ccagccaccc cagcgcgccg ccgcatttgc agccagcaga
     1081 attgcatcgg gtgctcgccg caccgagtcg cgcacaatcg gctcgagcat ttgactcgcc
     1141 cgccagggca gcccccgatc gagcgccaat tgcgccgcaa tgagtacgga gtcgggaccg
     1201 gaaccagcaa ccggatgctc cgagtcaccc agcaacgtgg attgtgctgc gaggggccgc
     1261 gcaacgagaa gcgcggctat gatgagtgtg cgggtcacgc aacaaatgtc gccgaggcgg
     1321 cggtgccttc gaaagggccg tgagtcgcct gcgaccatcg taactcgacc gtatcattgg
     1381 gaatgaccga ggaagagcgg gttcgcatcg acaagtggct ctgggccgcg cggtttttca
     1441 agacgcggtc gctggccagc gaggcggtgg ccggagggaa ggtcgaggtg aatggggagc
     1501 gtgccaaacc agccaagctg gttcgcccgg gagatgaggt gcgcctccgg gccggaccat
     1561 acgagtacgt gctagtggta cgaggcctgg cggagcgccg cggctcagca aaggtggccg
     1621 cgacccttta cgaggagcgg cccgagagcc gtgcggaacg ggagcgggtg gcgggccagc
     1681 tgcggctcgg tgcagccgcg tggacctatg aggaaaaggg gcggcccacc aagcgggatc
     1741 gtcgggcgtt ggaaaagttc aggcgacggg attagaaaca gcctgtcgag ctacatgttc
     1801 tcgagcatcg ccctggcgaa cccgctcgtc gacaccaacg tcgcgccgga catcagccgc
     1861 tcgaagtcgt aggtcacctt cttctgcccg atcgtccgct ccagcgacga gacgatgaga
     1921 tcggccgcct ccgaccagcc caggtgtcgc agcatcatct cgccggatag aatgagcgac
     1981 cccggattca ccttgtcctg gcccgcatac ctcggtgccg tgccgtgggt tgcctcgaac
     2041 accgcgtgcc ccgtgacgta attgatgttg gcgcccggag cgatcccgat cccgccgacc
     2101 tgggcagcta atgcgtccga gatgtaatca ccgttcagat tcatggtcgc gatcacgcca
     2161 taatcgcgcg gccgcgtgag aatctgctgc agaaacgcgt ctgcgatcac gtccttgatc
     2221 accacaccgt tcggcaactt gcaccacggg ccgccatcta cctcggtcgc gccgaactct
     2281 tcctttgcca actgatagcc ccagtcgcgg aagccgccct cggtgaactt catgatgttg
     2341 cccttgtgga cgagcgtcac actcgcaacg ccctcacggc tcgcgtaatc aatggccgcg
     2401 cggatgagcc gtttgctccc ctcttcagag atcggcttga tcccgatggc gctcgaatcc
     2461 gggaaccgga tggtttgggc tcccatttct tcctggagaa accggatgag cttgcgtgcg
     2521 tcctccgacc gtgccttgta ctcgatgccc gcatagatgt cttccgtgtt ctctcggaag
     2581 atcaccatgt tgacgtcctg cggcgccttg acaggcgatg gtacgccctc gaaataccga
     2641 acgggacgga cgcaggcgta gaggtcgagc ttctgccgga gagcgacatt gagcgaccgg
     2701 aatccgccgc ccaccggcgt agtaagcgga cctttgatcg ccaccaggtg atgcgagatt
     2761 gcggtgagcg tgtcgtccgg cagccagttg ttcaaacggt tcttggcttt ctcaccagcg
     2821 tacacctcga accagacgat gcgtcggtct gagccgtagg ccttcgtcac agctccatcg
     2881 aagacctgct gcgctgcgcg ccagatgtca gggccggtgc cgtcgccctc gatgaatgga
     2941 atgatcggat ggctcgggac ctccagcttg ccgtcgcgca tggcgatcga ctcgccgtcc
     3001 gttggggtcc ggagctcctt gtatgcgggt gccgaaagga tggacatacc gaatgccagc
     3061 gaaggtggtg gtttcgtgaa gggtctaagt tagccaccct cctatgaacg gcaacgttcg
     3121 accggaaccg gcggcagcgg gaatcttcgg cgagtaccag ctttggtaca ctgaattcgc
     3181 cgaggtcact cgtcgcgccc gccaacgatt ccagagtcgc gattggcacg gcacccaggc
     3241 agatgctgcc gagcggcttg ctctctatcg tctgcacgtg gagaccgcga cggcctatct
     3301 gcatcaggtc ctggacggcg acatccagga ccggaagacc tggacccgcc tccgatcctt
     3361 gtacgctggc ctcactgaag gccgcgatga tgccgagctg gccgagacct tcttcaactc
     3421 ggtgagccga cgcgtgcttg gcaccatcgg tgcgacctct gacgccgagt tcatcgagtt
     3481 ccccgccagc gggccgctgg cacgaaccgg tcccatgctg ttcgacacct atcgcggcga
     3541 gggcaccagc cgggatctgg taagccgatt gctggcggcg tgcggttggg acgttcccta
     3601 cgcaaatgcg tcgcacgacg tagaactggt cgcgagcgtc atcgacaaca ggctgtgcga
     3661 gctgcgctgg gagcgcggcc cgttggccat cgacgtgctc cgatcgcctt tttaccgcaa
     3721 caagggggct tacctcatcg ggcgggtcca cggagccaac gagctgctcc cgctggtcgt
     3781 tgctctggtc cacggcaaag aaggcattct ggttgatgcc gtcctgctca ctcaggacga
     3841 agccagcgta gtgttcgggt tcagctggtc ctacttcttc gtcgaggccc ctcaccctcg
     3901 cggtctcatc acgttcctcc aatccatcat gccgcataag cggatcgacg agttgtatac
     3961 gtcgattggc tacagcaagc acggcaagac cgagctgtac cgctcgctca tgcgccacat
     4021 ggaacgcccc gaggcccgtt tcgccctcgc cgaaggtgac gaaggaatgg tgatgtgcgt
     4081 cttcgcgctc cccagcttca acctggtgtt caagatcatc cgcgatcgct tcggcgcgcc
     4141 aaagaacacg acgcggcgcg ccgtcatgga tcgctatcac ttcgtgtttc agcgggatcg
     4201 ggtggggcgc ctggcagatg cccaggagtt cgaaaacctg gagcttcggc ggcgctgttt
     4261 tccggaggat ttgctggcct atttgaaaag ccacgccggc cggagcgtcg aggtgaagga
     4321 cgagagcgtt ctgctacgtc acgtctacac cgagcgccga gtgacgcccc tgaatctgtt
     4381 cctgctccag gccgacgcgg gtaccgctcg ggaaaccatt ctggactacg gcaacgccat
     4441 caaggacctg gccggcgcca acatcttcac cggcgacatg ctgctcaaga actttggcgt
     4501 tactcgaaac ggacgggtga tctgctacga ttacgacgag ctatgcctgc tcaccgactg
     4561 caccatacga cgcttgccgc cggctcgcac gccggaagac gagatggcag ccgagccctg
     4621 gttccacgtt ggcgagcacg acgtatttcc tgaggaattc gcggcctttc ttattcccgg
     4681 cgatcggctt cgaggcacat ttctcggcgc ccacagcgat ctgctcacgc ccgacttctg
     4741 gaccggtgtt cagcgccggc tcgaggcggg agagatcttc gacttcttcc cttacaaaca
     4801 ggcccgacgc cttcatcgag atggcgcggg ttaggcgtcg atctcgcccg gccggagccg
     4861 agggtcgtct agcgagcggg cggggcggaa tccgcgagaa cggagagtcg tgcgccggcc
     4921 gggtcggcct gcatctgaac gatttttcga ccggagcgat cacggatctg gagcgatgct
     4981 ccgtgtgagt ctgtctggat gtgaaccagc cccttccccg atttgttgcc gatgtcgagc
     5041 agggttccga gggaatccgc gcggattacg gcacgcactg ccgaatcaga cagatgtccc
     5101 gcttgcagct tcgcaaactc ggcgcgaacc tcggcgctgt catgactccg ccattgatcc
     5161 gcgtgatcgg cgggcgcgaa cagagctcga accacaccgc cgggctcgaa agcaacctca
     5221 ccgatcaact tgaggttctg agccgccgaa tcagcggcgc ccacgcagcg gaatccgctg
     5281 ccgtcagcga atccttccgg ctcgaagagc gtcacccggc agtcgggcag ccgcgcggct
     5341 ggtaccgagt cgtccaggcg caccgtcagg cggataccgg aaacctcgcg gggggagcgc
     5401 ctcaggatgt cgagccggcg aacggtcccg atctgggtgc tatccaaccg gaacggcatg
     5461 atgaccaagg ggatgctgat cggacgggtc gacgccgcca gagcccggag ttcgcctatg
     5521 ctattgcgtg tcaaggtgat aacgcccata cccaagacga aaatcgccgc tgcaccgaga
     5581 gcgatccgaa gccagtaccg ttgcatgggt cacctcgcta tggaacagac tgagcagccc
     5641 cgtcggggcc gttgcaagta gaaacgaggc ttgtcggctg attgtttcac ttccctaccc
     5701 gcgctgcttg atttgacccg tgcccgtccc ggtccagtca atcgatctca acgctgacgt
     5761 cggcgagggc ttcggggtgt ggccgccgct ggccgatgcc gacctgttcc cactgatctc
     5821 ctccgctagc atcgcctgcg gatttcatgc gggcgatccc acactcatgc gcgagaccgc
     5881 catgctcgct tcccgacgtg gcgtcgtcgt gggagcgcac ccaggatacc ctgatcttcc
     5941 cggcttcggc cgccgcgagc taggcgcgtc ggcggctgag attcgcgact acgtcaccta
     6001 tcaggtcggt gcgatgatgg cctgcgcctt ggcgg
//
LOCUS       DASRJQ010000630         6585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2516598, whole genome shotgun sequence.
ACCESSION   DASRJQ010000630 DASRJQ010000000
VERSION     DASRJQ010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6585
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2516598"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VFB89_14260"
     CDS             complement(<1..445)
                     /locus_tag="VFB89_14260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HZM28507.1"
                     /translation="MPPKKPHPHHDPHPEYTFQVKWDGRTVAGVRRVSALRRTVTVID
                     YRQGNDPATTQKLPGSVQYEGITLERGITHDPAFASWAAQVGGTAPSLSFRKEVRLEV
                     YNARGQLFEAYKIHRCWPSEYVAVGGLETGSETGPFQSLTLENEGW"
     gene            complement(482..784)
                     /locus_tag="VFB89_14265"
     CDS             complement(482..784)
                     /locus_tag="VFB89_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HZM28508.1"
                     /translation="MVRSALLVRLEAKPGQEAAVAKFLQGGLPIVEQEPATITWYALK
                     LGPSTFGIFDTFPDDKGRQAHLAGQVAAALMAKAPELLASAPTIEKVDVIAAKLPR"
     gene            complement(841..1272)
                     /locus_tag="VFB89_14270"
     CDS             complement(841..1272)
                     /locus_tag="VFB89_14270"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZM28509.1"
                     /translation="MSAEHYRKLERLYGTARVSQWYGAAISIADGQAEVRFPIRPEFH
                     HAANAVHGSVYFRALDDAAFFAVNSRVEDVLVLTVSFTVHFARPVTNGELHAVGRVLH
                     SGGRLFVAESDLFDGHGQLLSHGSGVFTRSAIKLSSEIGYA"
     gene            complement(1269..1646)
                     /locus_tag="VFB89_14275"
     CDS             complement(1269..1646)
                     /locus_tag="VFB89_14275"
                     /inference="COORDINATES: protein motif:HMM:NF021525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2255 family protein"
                     /protein_id="HZM28510.1"
                     /translation="MAAARFSETLLRQIDRAKRLRIRAGTEPHRFIGIWAVVVEGRVF
                     VRSWSLKPRSWYRTFLLEPVGAIEVGGRKISVRAVRTRSDRLKAAVDRAYLAKYDTPG
                     AIKYAEDLGRPKSRDTTTELIPA"
     gene            complement(1649..2026)
                     /locus_tag="VFB89_14280"
     CDS             complement(1649..2026)
                     /locus_tag="VFB89_14280"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZM28511.1"
                     /translation="MQPLGTIIEPGGQIFVALRADEVLGTCAVIPHQTGIFELAKLAV
                     AAQARGQGLGRALVQACVSFARRRGIGRVVLVSSSRLGAALSLYESLGFRRRPLPPDV
                     PYATADVYMDLDLTSSPEPVEQD"
     gene            complement(2166..2543)
                     /locus_tag="VFB89_14285"
     CDS             complement(2166..2543)
                     /locus_tag="VFB89_14285"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZM28512.1"
                     /translation="MTDAAAYPYETRLNVQFSPLELIDVQAMADACTYRWFNQTLCRV
                     NESVIRLGVVQGEYHWHQHADEDEFFYVVEGRFLIDLEDRTVELTPKQGFVVPRGVRH
                     RPRAPERTVILMVEGAGIVPTGS"
     gene            complement(2597..3229)
                     /locus_tag="VFB89_14290"
     CDS             complement(2597..3229)
                     /locus_tag="VFB89_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28513.1"
                     /translation="MLPAIENRLANLPLELKPLADVLRRHYARGASVRPDGTVQIARA
                     PWMGSEAFALVLYPAADAAWVSAYGQRLGRPVPEAYARVLASVNGCFVFGLALYGLPP
                     SLQDRPPRHDHSLEPLDLAAANRYWAREYRGAGEEFHFGGRTLSPTENVGYFLTSDGS
                     VRSRSKDGEVLREWATIERLLTEELASVEAADRERPPGAPAPWSFGREQS"
     gene            3378..3707
                     /locus_tag="VFB89_14295"
     CDS             3378..3707
                     /locus_tag="VFB89_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28514.1"
                     /translation="MTAIQQRRSQPRHRRQRYQPTAAYALVDSAKRKGIGLDDAPGPW
                     RVGGRALPRRGRNDLPMIPLVTNGRTEVMVDTLERAAELSAFLNWCGIDDLNPVSDLR
                     PPRFDMH"
     gene            complement(3720..4166)
                     /locus_tag="VFB89_14300"
     CDS             complement(3720..4166)
                     /locus_tag="VFB89_14300"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZM28515.1"
                     /translation="MSPWVVTRAIAAPIDRVFDTVADITQFSRAIPHITKVEFLSEQH
                     SGVGTKFRETRLIKGKETTTELEVTELVNNDRVRLIADSHGTVWDSIFQVGRAGNGTA
                     LTLTMEARSRGLIPRVMTRLIRGMVQRALEADMNAVKLYCEQPRQV"
     gene            complement(4418..4570)
                     /locus_tag="VFB89_14305"
     CDS             complement(4418..4570)
                     /locus_tag="VFB89_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28516.1"
                     /translation="MIRKLANGRYRLYSRKPNPKTGKRRNLGTFKSRAAALKHERAVQ
                     FFKRRG"
     gene            complement(4592..4918)
                     /locus_tag="VFB89_14310"
     CDS             complement(4592..4918)
                     /locus_tag="VFB89_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28517.1"
                     /translation="MESAQTLTFIVLGAVLGAAGQGARAVVGIKKEIVAARSEGRKVG
                     EWFDGRELVISFVLGAVAGVLAAIWQYAPDVQITRSLLLGFAGAGYAGADFISGLMDK
                     WIPVSK"
     gene            complement(4955..5359)
                     /locus_tag="VFB89_14315"
     CDS             complement(4955..5359)
                     /locus_tag="VFB89_14315"
                     /inference="COORDINATES: protein motif:HMM:NF021612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase-like protein"
                     /protein_id="HZM28518.1"
                     /translation="MSNVLAVVLGVAIGLVVSWLYVLFWKVRYTAQIRGDAVQRSQTV
                     TSGKVHEQLIPYLPAFPYNPKDVRFLGSPVDLVVFDGLAEGRLRRIVFLEVKTGSAGL
                     TTRERCVRDVVQAREVAWAELRVPRPAIGDAV"
     gene            5476..5826
                     /locus_tag="VFB89_14320"
     CDS             5476..5826
                     /locus_tag="VFB89_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZM28519.1"
                     /translation="MSTDALSTTFAALADPTRRAILERLSSGAATVKELSAPFAMSGP
                     AISKHLRVLERAGLIARGREAQWRPCRLEAAPLKEVAEWTEGYRRFWEASYQRLDDYL
                     KRLQAKEGRHGHGK"
     gene            5810..6298
                     /locus_tag="VFB89_14325"
     CDS             5810..6298
                     /locus_tag="VFB89_14325"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZM28520.1"
                     /translation="MATANKPNPTTFTTPTDRELVITRVVDAPRTLVWDVWTSPEHLP
                     QWLLGPEGWTMPVCEIDLRPGGAWRWGWRREDGSQMEMHGIYREIVPPERLVSTEAWG
                     GDWPETVNTLLLTEENGRTTITQRILYPSKEARDAALQTGMKEGVTVSFDRLARYLRS
                     LA"
BASE COUNT         1243 a         2104 c         2030 g         1208 t
ORIGIN      
        1 cccagccctc gttctccagc gtcagggact ggaagggacc cgtttcggac ccggtctcca
       61 ggccgcccac cgccacatat tcggacggcc agcaacggtg aatcttgtag gcctcgaaaa
      121 gctgcccgcg cgcattgtat acctcgagac gaacttcctt tcgaaaactc agactcggcg
      181 cggtaccgcc cacctgcgcg gcccagctgg cgaaggcggg gtcatgggtg atgccacgct
      241 cgagtgtgat accctcgtac tgaacacttc cgggcagctt ctgtgtcgtt gcgggatcat
      301 tcccctgccg gtagtcgatc actgtgaccg tgcgccgcag ggccgacaca cgacgcacac
      361 ccgcaaccgt tcgcccatcc cactttacct ggaaggtgta ttccggatga gggtcgtgat
      421 gaggatgcgg tttcttcggc ggcatgggct ggtcgctttc gaaggcagtc cgggttcgca
      481 atcaacgcgg cagctttgcc gcgataacat ctactttttc gatggtcggg gcgctcgcca
      541 gcagctcagg ggccttcgcc atcaatgccg ccgcgacttg accagccagg tgcgcctgac
      601 ggcccttgtc atcgggaaac gtgtcgaaga tgccgaatgt ggatggcccc agcttgagtg
      661 cgtaccaggt gatggtggca ggctcctgtt cgacaatggg tagtccgcct tgcaggaact
      721 tcgctaccgc cgcttcctgt cccggcttgg cttcgagacg aaccagtaga gccgaccgaa
      781 ccatcggcga ggctttggta gtgggcatct gaatttctcc tgtatatctc tgtgagcgcc
      841 tcaggcgtat ccgatctcgg aactgagctt gattgcactc cgggtgaaaa cgccgcttcc
      901 atgactcaat agctggccat ggccatcgaa tagatccgac tccgctacaa acaggcgacc
      961 gccgctgtga agcacacgcc ccactgcgtg aagctcgccg ttcgtcaccg gtcgagcaaa
     1021 gtgtactgtg aacgacacag tcaacacgag cacgtcttcc actcgtgaat tgaccgcgaa
     1081 gaacgcggcg tcgtccagag ctcgaaagta caccgaccca tggacggcgt tcgccgcgtg
     1141 atggaactcc gggcgaatcg ggaatcggac ttcggcctga ccgtccgcaa tcgaaatggc
     1201 ggcgccgtac cattggctca cccgagcggt gccgtacaag cgctccagct tgcggtagtg
     1261 ctcggcgctc acgccggtat cagctctgtg gtcgtgtcgc gggacttcgg ccggccaagg
     1321 tcctcggcgt acttgatggc gcccggcgtg tcgtacttgg cgaggtaggc gcgatcgact
     1381 gctgctttga gccggtcgct ccgggtccgc acggcgcgaa cggaaatctt tcggccaccg
     1441 acttcgatcg cgccgacggg ctcgagcagg aacgtgcgat accagctccg gggcttgaga
     1501 ctccaggacc ggacgaacac ccggccttcc acaaccaccg cccagatacc gatgaaccga
     1561 tgcggctcgg tcccagctcg aatccgcaat cgcttggcgc ggtcgatctg gcgaagcagc
     1621 gtctcggaga atcgggcggc agccatgctc agtcctgctc caccggctcc ggcgacgacg
     1681 tcaggtcgag gtccatatag acatcggccg ttgcgtaggg cacatccgga ggcaaagggc
     1741 gacgtcgaaa gccgaggctt tcgtacaggc tgagcgctgc ccccagccgc gagctggaaa
     1801 ccaggacgac acgaccgatg ccgcgccggc gcgcaaaact gacgcaggct tgcactaatg
     1861 cccggccgag cccttgacct cgagcctgtg ctgccaccgc aagctttgcc agctcgaaga
     1921 tgccggtctg gtgcggaatg acggcgcatg tgcccaggac ttcgtccgcg cggagcgcca
     1981 cgaagatttg cccgcccggc tcgatgatgg ttcccagggg ttggatagct gcgcttcatc
     2041 gggcgcttcg tatagcccgt ggtcgaccag gcaggcccga ttgagggcct cgaaagccgc
     2101 cgcgtgctcg gcccggaaga cgtcgattcg gacgccattg atgggctcgc cgttcacgac
     2161 acgactcagc ttccggtggg aacgataccg gcgccttcca ccatgaggat cactgtccgc
     2221 tccggcgccc ggggccgatg ccgcacgcct cgcggcacta cgaagccttg cttcggcgtg
     2281 agctcgacag ttcgatcctc caggtcgatc agaaagcgac cctccacgac atagaagaac
     2341 tcatcttcat cggcgtgctg gtgccagtga tactcgcctt ggacgacgcc gaggcggatg
     2401 acggactcgt tgacccggca aagggtctga ttgaaccagc gataggtgca ggcgtcagcc
     2461 atcgcctgta cgtcgatcag ctcgaggggc gagaactgga cgttgagccg ggtttcgtac
     2521 ggatacgctg cggcatctgt catggatcac tccgaccagg cggttgggca ttccttgatc
     2581 tagcgacgtt ccttatctag ctctgttcgc ggccgaaaga ccagggcgcg ggagcaccgg
     2641 gcggccgttc tcgatccgcg gcttccaccg aggccaactc ttccgtcagc aatcgctcga
     2701 tcgtcgccca ctcccgcagc acctcgccgt ccttgctgcg gctgcggacg gatccatcag
     2761 aggtcagaaa gtatccgaca ttttcggtgg gactcaacgt ccggccaccg aagtgaaact
     2821 cctcgccggc cccccggtac tcgcgggccc agtaccgatt ggcggccgca aggtcgagcg
     2881 gctcgaggct gtgatcgtga cgcggcggcc gatcctggag cgacgggggc aggccgtaaa
     2941 gcgccaggcc aaacacgaag cacccattca ctgacgccaa cactctcgca tatgcctcgg
     3001 gcaccgggcg acccaggcgt tgcccatacg cgctcaccca ggcggcgtcg gcagcggggt
     3061 aaaggaccaa ggcgaacgct tcagatccca tccatggcgc ccgcgcaatc tgcaccgtgc
     3121 cgtccggccg cacgctcgct ccccgagcgt agtgacgtcg cagcacgtca gcgagcggtt
     3181 tcagctccag cgggaggttg gcaaggcgat tctctattgc aggcagcaca gatgggttcc
     3241 ttttcgcaac tgaacctccg tgacggagtc ggccgatgtg attgatctca ctgccgcccc
     3301 acccgctgcg ttctacccta aaaatgtcgc gggatgcccc gccgtgtgac ccagatcgca
     3361 cgaaagtgag tcgaccaatg acggccattc agcaacgtcg ttctcaaccg aggcatcggc
     3421 gtcaacgtta ccagccgact gccgcgtacg cgctggtgga tagtgcaaag cggaagggga
     3481 ttggcctcga cgacgcacca ggaccgtggc gggtaggtgg tcgcgccttg cctcggcgcg
     3541 gccgcaacga tctcccgatg attcccctcg tcaccaacgg acgcaccgag gtgatggtcg
     3601 atacgctgga gcgggcagcc gagctgtcgg cgtttctcaa ctggtgcggc atcgacgatc
     3661 tgaacccggt gtccgatctg agaccgcccc gcttcgatat gcactaagct cgacagcggt
     3721 caaacctggc ggggctgttc gcagtaaagc ttcactgcgt tcatatccgc ttcgagcgcg
     3781 cgctgcacca taccacggat aagtcgcgtc atgactcggg ggatcagccc ccgcgagcgg
     3841 gcctccatcg tcagcgtaag agcagttccg ttcccggccc gccccacctg aaagatgctg
     3901 tcccagacag taccgtggct gtccgctatc agtcggactc gatcgttgtt cacgagctcg
     3961 gtcacctcga gctccgtcgt cgtttccttg cccttgatca gccgggtttc ccggaacttc
     4021 gtaccaaccc cggaatgctg ctccgacagg aattctactt tggtgatgtg cggaatcgcc
     4081 cgcgagaatt gggtgatgtc ggcgacggtg tcgaagactc tgtcgatcgg ggctgcgatg
     4141 gctcgggtga ccacccacgg cgacacaggt acctccggta gtagtccgtg atgaagaacg
     4201 tacgggggtc tgccacatgc gaaaatctcg ctcctcgaat ccgcatttga tcaccagcga
     4261 ttcgccgtct tccctgagcc gagccagaaa gttgccgcgg acccggaagg ccggtgtgcc
     4321 gtagcaggaa ccctcctcga cgccggggaa ggcaagcgcc agcattcgca ccgcctcgaa
     4381 ggttaaggcg gcattgcgtt ggcgcggggc cacggcgtca gccccgtcgc ttgaagaatt
     4441 gcacggcgcg ctcgtgcttc agagccgcag cccgtgactt gaaggtgccg aggttgcgtc
     4501 gcttgccggt cttggggtta ggcttgcgag agtacaggcg gtaccgaccg ttcgcgagct
     4561 tgcggatcat gacacatctc ctccggtcaa cctacttgct caccggtatc catttgtcca
     4621 tcagaccgct gatgaagtct gcgcccgcat atccggcgcc ggcgaatcca agcaagaggc
     4681 tccgggtgat ctgaacgtcg ggcgcatact gccagatcgc cgcgagcacg ccggcaaccg
     4741 cgcccaggac gaagctgatc acgagctccc ggccatcgaa ccattctccg acttttctgc
     4801 cttcgctgcg agcggccacg atttccttct tgatgcccac gacagctcgc gcgccttgtc
     4861 cggcggcgcc aagcaccgcg ccgagaacta tgaaggtcag cgtctgtgcg gattccatat
     4921 tgctgctccg tttgcaggtg cactgcgagc tacttcacac cgcgtcgccg atcgccgggc
     4981 ggggaacccg aagctcggcc caggcgactt cccgggcctg aactacgtcg cgaacgcagc
     5041 gttcccgggt cgtcaacccg gcgcttccgg tcttgacctc gagaaaaaca atgcgccgaa
     5101 gacggccttc tgccagtccg tcgaacacca caagatcgac cgggctcccg agaaagcgga
     5161 catccttggg attgtacgga aacgcgggca ggtaggggat cagctgctcg tggaccttgc
     5221 ctgaggttac ggtctggctg cgttgcacgg cgtcgccgcg aatctgcgcc gtgtagcgaa
     5281 ctttccagaa gagcacatag agccagctca cgaccagccc gatcgccact cccagcacca
     5341 cagcgagcac gtttgacacg ggagcctcga cttgacggtg gtttcgatgt gttgtatata
     5401 accgctaggt taattaacta tatggttatg tagggagact tgaaaacacc ggactgaagg
     5461 gcacctcgaa aaactatgag tacggatgca ctgagcacca cgtttgccgc gttggctgat
     5521 cccacacgtc gcgccatcct cgaacgcttg tcgtcgggcg cggcaacagt gaaagagctt
     5581 tccgctccct ttgcgatgag cgggcccgca atctccaagc atctccgagt gctcgagcgc
     5641 gccggcctga ttgcacgggg gcgtgaagcc cagtggcgtc cctgccgatt ggaggcggcg
     5701 cctctcaagg aggtcgcgga gtggaccgag ggataccgcc ggttctggga agccagctat
     5761 cagcgtctgg atgattacct gaaacgctta caagcaaagg agggccggca tggccacggc
     5821 aaataagccc aacccgacca cgttcacgac tccgaccgat cgcgagctcg tcatcacccg
     5881 ggtggttgac gccccgcgca cactggtgtg ggacgtgtgg accagcccgg agcacctgcc
     5941 tcaatggctg ctcggaccgg aaggctggac catgccggtt tgcgagatcg acctgcgacc
     6001 cggcggagcc tggcgctggg gatggcgtcg cgaggatggg agccagatgg aaatgcatgg
     6061 catttacaga gaaatcgtcc cacccgagcg gctcgtctct accgaagcat ggggtggcga
     6121 ctggcctgaa accgtcaaca cgctgctcct gaccgaggag aacggcagga cgacgatcac
     6181 gcagcgtatc ctttatccgt cgaaggaagc gcgggatgcc gcgctccaga ccggaatgaa
     6241 ggaaggcgtg actgtcagct tcgatcggct cgccaggtac ctccggtcgc tggcttgacc
     6301 ggggtctgga accgggcggt ttgccgccga tccgcaaggg tcaggcggcg ctgctggccc
     6361 gtcggacgca aactgacaag gtccggcggg caagcctgac gttcctcctc catagccagt
     6421 gactgcgcct gcggcgccgc atttataacg tggtacccga gatgaatgct ggattcagaa
     6481 cctgggggtc acgatgcaag tggaatttcg cggcgactgg caaatccaag tgtgcaccaa
     6541 tccgagctgt ggcgtcggtc ctcttcgcgt gcggcgcgac ccccg
//
LOCUS       DASRJQ010000631         2973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1362471, whole genome shotgun sequence.
ACCESSION   DASRJQ010000631 DASRJQ010000000
VERSION     DASRJQ010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362471"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="VFB89_14330"
     CDS             complement(<1..535)
                     /locus_tag="VFB89_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28521.1"
                     /translation="MSLSQNIFSWFRSPPTHNRRIAVALGVACLSAAYVALVYSVQPP
                     GTAADFDQWWIGAHALRQGKDPYLAVAGSGWSWPLFYPLPAVLCAVPFTVLPLPAARS
                     AFVALGAFVLAYAVTWRVWWPLVLFTSGAWLATAVAAQWTPFLVAGALLPVVGVLYVV
                     KPNIALASWIYRPSWIPV"
     gene            complement(532..945)
                     /locus_tag="VFB89_14335"
     CDS             complement(532..945)
                     /locus_tag="VFB89_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28522.1"
                     /translation="MRFGLILLLALIGARVEGQDRDLEREADRARRAWFDHDASALVA
                     NAPRLVIRLPGADPSGALGQAQAAALLRDFLAPSQEVETAVRSAEEVEPGRGYVELQR
                     RYKVAGTQEVRSQTLLLAYRRGSVGWVLVELRVTT"
     gene            complement(930..1619)
                     /gene="pyrF"
                     /locus_tag="VFB89_14340"
     CDS             complement(930..1619)
                     /gene="pyrF"
                     /locus_tag="VFB89_14340"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HZM28523.1"
                     /translation="MTEIVLALDIGAPDTARRMLDQVPDLRWVKLGPVLMTRVGAPFV
                     QEVAARGLQVFLDLKWHDIPNTVAGAVAAARDLGVAMATVHTLGGSGMLEAAAQAGGG
                     DLTLVGVTVLTSHDRTSYAAAIGRAQGDLEVEAERLAKVARSAGLGGVVCSPREISRV
                     RRVWSDGVIVVPGIRRGSDPKDDQARVSTAGDAARDGATHLVVGRPLLQATNPASVLE
                     ELREEVACALA"
     gene            complement(1616..2488)
                     /locus_tag="VFB89_14345"
     CDS             complement(1616..2488)
                     /locus_tag="VFB89_14345"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HZM28524.1"
                     /translation="MAAVELSRELFGQRFQNPLLLASGTAGFGRELNGVMDLDRLGGL
                     VTKAVSVAPRKGNAPPRVAEFRAGMLNSVGLANPGLDSFRQVELPWLAGHLRRARVLV
                     NVVGFTIDEYAEVVQGLDGAAGIVAFEINLSCPNTSAGGIEFGADPRCVAQIVGACRT
                     RTRLPLVAKLSPVLPDLAGMAEVARDSGADAVSVVNTLPGELFDAKGATRLGNGFGGV
                     SGPALLPVGLLAVARVVERSPGLPVIGVGGVRSADDVRQYLRAGASLVAIGTAALADP
                     RLPERIVHDLETGL"
     gene            complement(2488..>2973)
                     /locus_tag="VFB89_14350"
     CDS             complement(2488..>2973)
                     /locus_tag="VFB89_14350"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HZM28525.1"
                     /translation="SLPRRPGFTDTRGFETYFLAEARTEDAARVARMENEAVRFENVG
                     DGATQLGAVDFIVKDLQLNEHLRESARLAELVQKDLGQVHTGPDMGVKQAGFMVLTTA
                     RRPAVLVEMGYSTNRDDSKFLTSMAGQRSIASAIADAVVEYLLEYERKTDSAADSGTT
                     R"
BASE COUNT          574 a         1031 c          918 g          450 t
ORIGIN      
        1 cgaccggaat ccaggaggga cgatagatcc agctcgccaa ggcgatgttc ggcttgacga
       61 catagagaac gccgacaacc ggcagcaatg ccccggccac cagaaacgga gtccattgag
      121 ccgcaacggc cgtcgcgagc caggctcctg aggtaaacag cacgagcggc caccagactc
      181 gccaggtgac cgcatacgcc agcacaaacg cgccgagtgc cacgaacgct gaccgagccg
      241 cgggcagcgg cagcaccgtg aagggcaccg cgcataagac tgccggaagc ggatagaaca
      301 acggccagga ccatccggac ccggccacgg cgagatacgg gtccttgcct tgacggagcg
      361 catgcgcgcc gatccaccac tgatcgaaat cagccgcggt cccagggggt tgcaccgagt
      421 aaaccagcgc aacgtaggca gcgctcaggc aggcgacccc cagggcgact gcgatccgtc
      481 ggttgtgagt gggcgggcta cggaaccagc tgaaaatgtt ctgggaaaga ctcacgtagt
      541 gacgcgaagc tcgaccagca cccagccgac gcttccgcgc cggtacgcaa gcaggagcgt
      601 ctgcgaccgt acttcctgag ttcccgcaac tttgtaccga cgttgcagct cgacgtatcc
      661 ccgccccggc tccacttcct cggccgatcg cactgccgtc tccacctcct gtgacggagc
      721 caggaagtct cgaaggagag ccgcggcctg tgcctgaccc aacgctcccg acggatccgc
      781 acccggcagg cgaatgacca ggcgaggcgc atttgctacc aatgctgatg catcatgatc
      841 gaaccaggcc cgccgtgcgc ggtcggcttc acgctcgaga tcgcggtcct gcccctcgac
      901 tctggcacca atcagcgcga gaagcaggat caggccaaag cgcacgccac ctcctcgcgg
      961 agttcttcga gcacacttgc cggattggtc gcctgcagga gcggtcgtcc gaccaccaga
     1021 tgggtggcgc cgtcccgtgc cgcgtcaccc gcggtggaca cgcgggcctg gtcgtccttg
     1081 gggtcggaac cccgccggat accgggcacg acgatcaccc cgtcactcca gactcggcgc
     1141 actcgggaga tctcccgtgg cgaacagacc accccgccaa gccctgccga ccgggcaacc
     1201 ttggccaacc gttccgcctc gacttcgaga tcgccctgtg cacgaccgat tgcggcagcg
     1261 taggatgttc tgtcgtgaga ggttaggacc gtcaccccga ccaaagtcag atccccgcca
     1321 cctgcttggg ctgctgcttc cagcattccc gatcctccca gtgtgtgcac cgttgccatg
     1381 gcgactccga gatcgcgggc cgcagctacg gcgccggcca cagtattggg aatgtcgtgc
     1441 cacttgaggt cgagaaagac ctgaaggccg cgggccgcca cctcttgcac gaatggggcc
     1501 ccaactcgag tcatgagtac cggtcccagc ttgacccatc gaaggtccgg cacctgatcg
     1561 agcattcgac gcgctgtgtc cggcgcgccg atgtcgagag cgaggacgat ttcagtcata
     1621 agccagtctc cagatcatgc acgatgcgct cgggtaggcg cgggtcagcc agtgcggccg
     1681 tgccgatggc aaccagcgag gcgccggccc gaagatactg acgtacgtcg tcggcgctgc
     1741 ggacgccgcc gaccccgata acaggaaggc ctggactccg ctcgaccact ctcgcgacag
     1801 cgaggagccc gaccggaagg agcgcgggcc cgctgacccc gccgaatcca ttgcccaggc
     1861 gcgttgctcc cttcgcgtcg aacaactctc cgggcagcgt gttgaccacg cttaccgcgt
     1921 ccgcgccgga gtcccgtgcc acctccgcca tgccggccag atccggcagg actggcgaga
     1981 gcttggcgac tagcggcaga cgggttcgag tccggcaggc cccaacgatc tgcgcgacgc
     2041 agcggggatc cgcgccgaac tcgatgccac cggcgctggt gttgggacaa gagagattga
     2101 tctcgaacgc gacaatccct gccgccccat ccagaccctg tacgacctcc gcgtactcgt
     2161 cgatcgtaaa gccgaccaca ttcaccagga ctcgtgcccg gcgaagatgc ccagccaacc
     2221 agggcagttc gacctggcgg aacgaatcga ggccgggatt ggccagcccg accgagttga
     2281 gcatgcccgc cctgaactcc gccacccggg gcggagcatt gccttttcga ggcgcgaccg
     2341 agaccgcctt ggtgacaagg ccacccagcc ggtcgaggtc catgaccccg ttcagctcac
     2401 gaccgaaccc ggctgtgccg gaggcgagga gcaggggatt ttgaaatcgc tgaccgaaga
     2461 gttcgcggga gagctcgacc gcagccacta gcgagtcgta ccggagtcgg ccgcggaatc
     2521 ggtcttccgc tcgtactcca gcaggtattc gacgacggcg tcggcgatcg cgctcgcaat
     2581 ggagcgctga cccgccatgg acgtgaggaa cttgctgtcg tcgcggttgg tactgtatcc
     2641 catttcgacc agcacggccg ggcgacgggc cgtggttagc accatgaagc cggcctgttt
     2701 cacacccata tcaggcccgg tgtgcacctg gccgagatcc ttctgcacca gctccgccag
     2761 ccgggcggac tcccgcaagt gctcattcag ttgcagatcc ttcacgatga agtcgaccgc
     2821 gccaagctgg gtcgccccgt caccgacatt ctcgaatcgg acggcctcgt tctccattcg
     2881 cgcaactcgg gccgcgtcct ccgtcctggc ctcggcaaga aagtacgtct cgaacccgcg
     2941 agtgtcggta aagccggggc gccgaggcaa cga
//
LOCUS       DASRJQ010000632         2717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_390259, whole genome shotgun sequence.
ACCESSION   DASRJQ010000632 DASRJQ010000000
VERSION     DASRJQ010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390259"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VFB89_14355"
     CDS             complement(<1..777)
                     /locus_tag="VFB89_14355"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HZM28526.1"
                     /translation="MGTFRQTPALTFDDVLLVPRHATVHPRHVDVKSRFTRDIELQIP
                     LISAAMDTVTEAEMAIAIARAGGIGVIHKNMTIERQAAEVDRVKRSESGMILNPITLG
                     PDRQVREAAQLMARFRISGVPIVDSAGKLVGIITNRDLQFERALDRPIREAMTKDKLV
                     TAPVGTTLDEAERILGQHRIEKLPVVDQQGVLRGLITIKDIFKRRQHPNANKDQHGRL
                     RVAAAVGGTPDATTRAKSLLDAGCDVIVVDSAHGHSQGVLD"
     gene            complement(815..1339)
                     /locus_tag="VFB89_14360"
     CDS             complement(815..1339)
                     /locus_tag="VFB89_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28527.1"
                     /translation="MKHAPGSQVALVVFGLVVASCGRGADRESRTSQAAKSSDSQPAA
                     AAPTGACGYISEEDASSALDQPSRYRRGQPSTQACTVEPASGDVFHGTTVEYRLTQGS
                     TAQYDFIAAQKPAQPISGLGDRALWLPAGDTRGNLVVVRGSTIVNMVITDLDRHGDLQ
                     KRARAFAKKVLERL"
     gene            complement(1336..1716)
                     /locus_tag="VFB89_14365"
     CDS             complement(1336..1716)
                     /locus_tag="VFB89_14365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28528.1"
                     /translation="MNDLLRERLLRRLEALPEEKVYQILDYVEFLESKYAERPAGAPA
                     FQKVAETLEDTMRAGRVPITIIKGTMDAVGKAGKFLGGLAAAGKAAVEDARKKTADAP
                     ATAETTTTPTPTPVPTPTPPQTES"
     gene            complement(1713..>2717)
                     /locus_tag="VFB89_14370"
     CDS             complement(1713..>2717)
                     /locus_tag="VFB89_14370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28529.1"
                     /translation="LRLLPVVVLLAAPLTAQARVRVSSDGAWFYQAASGRRLAQLEAG
                     AVLAQGSAQGDWVQVTLDGWIFGSSVGSTTRDGFDLAVTKSPSENLRAEPAGDVVAHL
                     SKGFLLNKQTEQAGWVRVRRTGWMQRSALTAVAAPQTANAGQSALPPGPRPGGVQDTS
                     APTAVDARRVQAARATALYRVPDATTDATLSANAPLRVLGRAGEWTRVSLEGWVKTAD
                     LQSAAPGVLLGVSAAELRAEPDRYVGVTLRWTLQVIALRTADDLRPDIPDGATYLLAR
                     GPTPERGFVYVVVPQARRAEVEALGPLASVQATVRVRTGRSRFIGNPVVDLVSLEVQP
                     "
BASE COUNT          409 a          923 c          925 g          460 t
ORIGIN      
        1 gtcgaggacc ccctgcgagt gaccgtgcgc ggaatcgacg acgatcacgt cgcagcccgc
       61 atcgagcagt gatttcgcgc gtgtcgtggc gtcgggcgtg ccgcccacgg ccgcggccac
      121 gcgcaggcgt ccgtgctgat ccttgttggc gttgggatgc tggcgccgct tgaagatgtc
      181 cttgatggtg atcaagccgc gcaggactcc ctgctggtcg accaccggca gcttttcgat
      241 gcggtgctgc ccgaggatcc gttcggcctc atcgagcgtg gtgccgaccg gcgcggtgac
      301 cagtttgtcc ttggtcatcg cctcgcggat cgggcggtcg agcgcgcgct cgaactgcag
      361 gtcgcgattg gtgatgatgc cgaccagctt gccggccgag tcgacgatcg gcacgccgga
      421 gatgcggaag cgggccatca gctgggcggc ttcgcgcacc tgccggtcgg ggcccagcgt
      481 gatcgggttg aggatcatgc ccgattcgct gcgtttcacg cggtccacct cggccgcctg
      541 gcgctcgatg gtcatgttct tgtggatgac tccgatcccg ccggcgcgcg cgatggcgat
      601 cgccatctcg gcttcggtca cagtatccat ggccgccgag atgaggggga tctgcaattc
      661 gatgtcacgg gtgaagcggc ttttgacgtc gacgtgacgc ggatgcaccg tggcgtgccg
      721 cggtacgagc aggacgtcgt cgaaggtgag cgcgggggtt tggcggaagg ttcccatggc
      781 ggaagttatc gcagcccggg gggcttctca acacctacag gcgttccagc accttcttcg
      841 caaacgctcg cgcccgcttc tgcagatcgc cgtggcggtc gagatccgtg atgaccatgt
      901 tcacgatggt cgacccacgc accacaacca gattcccccg cgtgtcaccg gccggcagcc
      961 acagcgcgcg gtccccgagg ccggatatcg gctgggcagg cttctgcgcc gctatgaagt
     1021 cgtactgcgc ggtgctgccc tgcgtcagcc ggtactccac ggtcgttccg tggaagacgt
     1081 cgccggatgc gggctcgacc gtacaggcct gagtgctggg ctggccgcga cgatagcgcg
     1141 aaggctggtc gagcgccgag gatgcatcct cctcgctgat gtatccacac gctccggtgg
     1201 gcgccgcggc ggccggctgc gaatcactgg acttggccgc ctggcttgtg cgggattcgc
     1261 ggtcggcgcc gcggccgcag ctcgcaacga caagaccgaa taccaccaac gcgacctggc
     1321 tgccgggtgc gtgcttcatg actcagtctg cgggggagtg ggagtgggaa cgggagtggg
     1381 agtgggagtg gtagtggtct ccgcggtggc cggtgcgtcc gcggtcttct tcctggcatc
     1441 ttccaccgcg gccttccccg ccgcggccaa tcccccaagg aacttcccgg cctttcccac
     1501 cgcgtccatc gtgcccttga tgatagtgat cgggacccgg cccgcccgca tcgtatcctc
     1561 cagcgtctcg gccaccttct ggaacgcggg agcgccggcc ggccgctcgg cgtacttgga
     1621 ctccaggaat tcgacgtagt cgagtatctg atacaccttc tcctccggga gcgcctcgag
     1681 ccggcgcagc agccgctcgc gcagaaggtc gttcatggtt gtacctccag cgaaaccaga
     1741 tcgacgaccg ggttaccgat gaaacggctg cggccggtcc gcacccggac ggtggcctgc
     1801 acggatgcga gcggcccgag cgcctcgact tcagcgcgcc ttgcctgtgg cacgacgaca
     1861 tagacgaagc ctcgctccgg cgtcggtcca cgcgccagga gataggtcgc gccatccggg
     1921 atgtcgggac gcaaatcgtc cgccgtacgc agtgcgatga cctgcagcgt ccaacgcagc
     1981 gtcaccccga cgtagcggtc gggctcggcc cgcagctccg cggcgctcac ccccagcagg
     2041 acgccgggag ccgcggattg gaggtcggcc gtcttcaccc aaccctcgag cgagacgcga
     2101 gtccattcgc ccgcgcgccc cagcaccctg agcggcgcgt tggccgagag cgtggcatcg
     2161 gtcgtagcgt cgggaacgcg gtagagcgcg gtggctcgcg cagcttggac ccggcgtgcg
     2221 tccaccgcag tgggtgcgct cgtatcctga acgccccctg ggcgtggccc agggggcaac
     2281 gcactctgac cggcattagc cgtttgtggc gccgccactg cggtcagcgc cgagcgctgc
     2341 atccacccgg tccgccgcac acgcacccag ccagcctgct cggtctgctt gttgagcaga
     2401 aatcccttgc tgaggtgcgc gacgacatcg cccgcaggct ccgcgcgcag gttctccgaa
     2461 ggcgacttgg tcacggccag atcgaaacca tcccgcgtgg tcgagccgac cgatgatccg
     2521 aagatccaac cgtccagcgt cacctgcacc caatcgccct gcgccgagcc ttgcgcgagc
     2581 acggcgcccg cttcgagctg cgccagacgc cgtccgctcg ccgcctgata gaaccaggcg
     2641 ccgtcgctcg aaacgcggac ccgggcttgt gcggtgagcg gtgcggcgag caggacgaca
     2701 actggtagga ggcgcag
//
LOCUS       DASRJQ010000633         2055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_192792, whole genome shotgun sequence.
ACCESSION   DASRJQ010000633 DASRJQ010000000
VERSION     DASRJQ010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192792"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VFB89_14375"
     CDS             <1..529
                     /locus_tag="VFB89_14375"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HZM28530.1"
                     /translation="LWRFIFDDQAGIVNSVLSSVGVIHRPIVWFIHPDLAWVPVIVSD
                     VWKTTPFVALLLLAGLQNIDSALYEAARIDGASAWQQFRHVTLPLLKPAILVALVFRT
                     LDSVRVFDLVYVLTGGGPGTSTEPIALFTFNSLLQNLRFGYGSALSVITFALSFVLAL
                     GYLRLLGRDLTEARA"
     gene            526..1347
                     /locus_tag="VFB89_14380"
     CDS             526..1347
                     /locus_tag="VFB89_14380"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HZM28531.1"
                     /translation="MKGRRWSAADVAIGILILASAFPFYWAIVSSFTPEARLFQKPSL
                     LPSGLVLDHYRTLFEQRGFWVPIRNSLIVAGTTTVFCIVVGSFCAYALARLRFRGKAP
                     LLALILTVSMFPQIAIVSPLYLVLRALRLINTYPGLILPYLTFATPLTVWLLVGFFRQ
                     LPVELEEAAMVDGASRLRIFWEVVVPVALPGLAATAILTFLYCWNEFLFALSFTLGPE
                     RQTVPVAIALFRGRYQVPWGQILAAAIVATAPVAALVLIFQRRIVQGLTAGAVKG"
     gene            complement(1380..1916)
                     /locus_tag="VFB89_14385"
     CDS             complement(1380..1916)
                     /locus_tag="VFB89_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28532.1"
                     /translation="MLVEGTAESPQVVARHIVELSDPDVPAARQPYHASTGLARELDS
                     PELHRLIADIQAYARNSVAELLNRIASSGRRLAGAGIAVGSEIDPARIGNPHVRAHAN
                     EGRLFRTVVQGALAERSLPSSIFVERELPGLAAQRLGQSEAALKRRLTELGRPLGGSW
                     RRDDKMAALAAWLVLASY"
BASE COUNT          301 a          658 c          656 g          440 t
ORIGIN      
        1 gctttggcgt ttcattttcg acgaccaggc agggatcgtg aactccgtct tgagcagcgt
       61 cggcgtgatc caccggccga tcgtgtggtt cattcatccg gatctcgcct gggttcccgt
      121 catcgtgtcg gacgtgtgga agacgacgcc gttcgtggcg ttgctcctgc tcgccggatt
      181 gcagaacatc gactccgcac tctatgaggc ggcccggatc gacggggcct cggcctggca
      241 gcagttccgg cacgtaacgc tgccgctgct caagcctgcg atcctggtcg cactggtgtt
      301 ccgcacactc gacagtgttc gcgtctttga cctggtgtac gtgttgaccg ggggaggccc
      361 gggaacctcg accgagccga ttgcgctctt caccttcaac tcgctgctcc agaaccttcg
      421 attcggctat ggatccgccc tgtcggtgat cactttcgcg ctgtcgtttg tcctggcgct
      481 gggctatctg cggctgctgg gccgggatct gacggaggct cgcgcgtgaa ggggcgcagg
      541 tggagtgcgg ccgacgtcgc gatcgggatt ctgatcttgg caagcgcgtt tccgttctac
      601 tgggcgatcg tgtcgtcgtt cactcccgag gcacgccttt tccagaagcc gtcgctgttg
      661 ccctccggtc tggtgctcga tcactaccgc accttgttcg agcagcgggg cttctgggtc
      721 ccgatccgga actcgttgat cgtggccggc accacgacgg tgttttgtat cgtggtcgga
      781 tccttctgcg cctacgcgct cgcacgactt cggttccggg gcaaagcccc gctgctcgcc
      841 ctcattctca cggtgagcat gttcccccag atcgcgatcg tttcgccgct ctatctggtg
      901 cttcgcgcgc tgcggctgat caatacctat cccgggctca tcctgccgta tctcaccttt
      961 gcgacgcccc tcaccgtgtg gctgctggtc gggtttttcc gtcagctgcc cgtggagcta
     1021 gaggaggcgg cgatggtgga cggggcgagc cggcttcgga tcttctggga ggtggtggtg
     1081 ccggtcgcgc tgccggggct ggcggcgacg gcgatcctca cgttcttgta ctgctggaac
     1141 gagtttctgt ttgctctctc gttcacgctg ggaccggagc gccagaccgt ccctgtagcc
     1201 atcgcgctgt tccggggacg atatcaggtg ccctgggggc aaatcctcgc ggccgcgata
     1261 gtggctacgg caccggttgc cgcgctcgtt ctcatcttcc agcgcagaat cgtccagggg
     1321 ctcacagccg gtgccgtgaa aggctaaacc gccgctcaca caagcctttt cggcgaacct
     1381 cagtaagaag cgaggacgag ccatgcggct aatgccgcca tcttgtcgtc ccgacgccac
     1441 gacccgccca gcggacggcc cagctcggtc aggcgtcgct tgagcgccgc ttcggattga
     1501 ccaagtcgtt gggctgcgag accgggaagt tcgcgttcga cgaagatcga ggagggaagg
     1561 gaacgctcgg caagggcccc ctggactacc gtccgaaaca gccgcccttc gtttgcgtga
     1621 gcacgcacgt gaggattgcc gatgcgggcg gggtcgattt cgctcccgac ggcaattccg
     1681 gcgcccgcga gtcggcgccc ggatgatgca atacggttga gcagctcggc gacgctattg
     1741 cgtgcgtacg cctggatgtc tgcaatcagc cggtgcagct ccggcgaatc caactcccgc
     1801 gcgagccccg tactcgcgtg atacggttgc cgagctgcgg gcacgtccgg gtctgaaagc
     1861 tccacgatgt ggcgggcgac gacctgcggc gattcggcgg tgccctcgac cagcacggcc
     1921 gctgcccacc ccgatttgac gcggaatccc agtgcggcgg tggcctgcag ctggcctcct
     1981 taccccagag tcttcagagt cttccggtat acatgctgac tcttgaatcg ctcgaagccc
     2041 agccgttgat aaaac
//
LOCUS       DASRJQ010000634         2025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_670525, whole genome shotgun sequence.
ACCESSION   DASRJQ010000634 DASRJQ010000000
VERSION     DASRJQ010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670525"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="VFB89_14390"
     CDS             complement(<1..1001)
                     /locus_tag="VFB89_14390"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZM28533.1"
                     /translation="MTVAPATPLSRYPARVIAQERPLRSERADVEPRRWAWMDNLRVA
                     VIAGVIVEHVATAYVLDIDWYYEERSANAVTEAIVGAAILPAALFAMAVLFLVAGLLS
                     TRSLARKGPWPFVRDRLLRLGAPLIAFALVIGPLTSLLGQWAEGASSADDVGSFLIAK
                     VQDADTGPMWFVTALLVFSIAYGAWRCARPAGSTASGPLRPWHLVVAAAAIVVGSIAV
                     RLQWPFTADTPFDLNLWEWPQMATLFALGALAGERGWLDPTPDWLRRACGRASIVGAL
                     GLFFVVAAVALAGDEEPFLGGWHLLALAEPTTEATIAVAMSFWLVGWFARRWNYQGTL
                     "
     gene            complement(998..1777)
                     /locus_tag="VFB89_14395"
     CDS             complement(998..1777)
                     /locus_tag="VFB89_14395"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZM28534.1"
                     /translation="MNHSVKDVVMDLARSRAQPRDRLEDLVRAGAMVFASRGYRRTQM
                     ADVAREMGVSPGNLYNYVDSKDALFYLVLRRMFGERPGDRPLELPVTGASVTVTSEWV
                     ATRLDFVSDFPELERAFARRRPARQDAEIEAIVGELYDVLARMRLGVDMIERSVEDLP
                     ELAEVFGELRRELFARYERYLRQREKVGTIGLEKPEAAAHLIVELCSWAAGRRPRDPQ
                     ASGISDSTARRAVCRFIADALTSGGGRNPASTHAIPKKDGT"
BASE COUNT          378 a          701 c          642 g          304 t
ORIGIN      
        1 agcgtgccct ggtagttcca ccgacgcgcg aaccatccca ctaaccaaaa ggacatggcc
       61 accgcgatcg ttgcttcggt ggttggctcc gctagggcta gaaggtgcca cccaccgagg
      121 aagggctctt cgtcgccggc cagcgccacg gccgcgacga cgaagaaaag acccagggca
      181 ccgacgatgc tggcccgccc acaagcccgc cgcagccaat ccggtgtcgg gtcgagccat
      241 ccacgttcac ccgccagtgc ccccaaggcg aacaaggtcg ccatctgggg ccactcccaa
      301 aggttcaggt cgaagggcgt gtcagcggtg aagggccatt gcagccgcac cgcaatcgac
      361 ccgacgacga tggcagccgc tgcgacgacg agatgccacg gccggagcgg tccagatgcg
      421 gtcgagccgg caggacgggc acagcgccag gccccatagg cgatggagaa caccagcagt
      481 gctgtcacga accacatcgg accggtgtca gcgtcttgca ccttcgcgat gaggaaggaa
      541 ccgacatcgt ccgcggacga ggctccttcg gcccactggc ccagcagact cgtcagcggg
      601 ccgatcacca aggcaaaggc gatgagcggt gcccccaaac gcaggaggcg gtcgcgaacg
      661 aaaggccacg gacccttgcg cgccagcgac cgtgtactga gcaggccagc aaccaggaac
      721 agcaccgcca tcgcgaacag ggccgccgga aggatcgcgg cgccgacgat ggcctcggtc
      781 acggcatttg cggaccgttc ctcgtaatac cagtcgatat cgagaacgta ggcggtggcg
      841 acatgttcga cgatgactcc cgcaatcacc gcaacccgga ggttgtccat ccatgcccag
      901 cgccgaggct ccacatcggc gcgttctgat cgcagtgggc gttcttgagc gatcacccgc
      961 gcggggtagc ggctcagcgg agtcgccggt gcaaccgtca tgtcccgtcc ttcttgggta
     1021 ttgcatgcgt ggacgcggga ttgcgcccac cacccgaagt cagtgcatca gcgatgaagc
     1081 ggcacaccgc ccggcgcgcg gtggagtccg agatccccga tgcctgcggg tcacgggggc
     1141 gacggcccgc cgcccacgag cacagctcga ctatgaggtg ggcagcggcc tcgggttttt
     1201 cgagcccgat cgtgccgacc ttttctcgct gtcgcaggta ccgctcgtag cgggcgaaca
     1261 gctcccggcg tagctcgccg aagacctcag cgagctcggg taggtcctcg accgaccgct
     1321 cgatcatgtc cacccccagg cgcatccgtg cgagcacgtc gtagagctcg cccacgatcg
     1381 cctcgatctc agcgtcctgc ctggcaggtc gcctccgcgc gaaggccctc tccagctccg
     1441 ggaagtcgct cacgaagtcc agtcgcgtcg cgacccactc cgaggtgaca gtgacgctcg
     1501 cccccgtgac gggcagttcc aacggccgat cacccggtcg ctccccgaac atccggcgga
     1561 gtaccaggta gaacagcgcg tccttactgt cgacgtagtt gtagaggttg cccggcgaca
     1621 cccccatctc ccgcgccacg tcggccatct gcgttcggcg gtagccccga ctggcgaaca
     1681 ccatcgcacc cgctcggacg agatcctcta gccggtctcg cggctgcgcc ctactgcggg
     1741 ccaggtccat gactacatcc ttgactgaat gattcattca gtcaaggaga gatcggaact
     1801 tggttcgtga tggccggtcc gaacgagcca gcgccggacc catgcactac ggaccgacga
     1861 tctggtagcg actggagcgc aggagcgatc ggattcgcac cgcaaccgcc ttcggggcgg
     1921 cctcgtcgtt catgctgcgc atgagctgag cagcgtcgta gagcgcctcc atctcggcgg
     1981 tcgccgcgtc gatgacggcc gacgacggct ggtcgccgac gtggg
//
LOCUS       DASRJQ010000635         2262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_501350, whole genome shotgun sequence.
ACCESSION   DASRJQ010000635 DASRJQ010000000
VERSION     DASRJQ010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501350"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(477..683)
                     /locus_tag="VFB89_14400"
     CDS             complement(477..683)
                     /locus_tag="VFB89_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28535.1"
                     /translation="MTELSAASRYVLDPNLAELIGILEASTPIILLLSMAAGVYLWSK
                     DHARRRRALRILRLLLGRRPEIRR"
     gene            918..2054
                     /locus_tag="VFB89_14405"
     CDS             918..2054
                     /locus_tag="VFB89_14405"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM28536.1"
                     /translation="MFQLLQRLHLEAGAPSRRDISKRLGQGVLSHTTVNGVLRRPAEV
                     KWGQLELVADALGGEIAELRQLWTAAQEERASGIVASGRAHASSESPMVGLFIGDLSD
                     WVDKAEHAFSAGDYIFAQRSAAVVAAAALAAEGEAARRLGHLERADAALREALDLQKA
                     NVGPNDPMTLLTRQRLARVMFQQGHLDNALGEITAVVEAWERLGARDDHRGLRARLEL
                     GFIQSMTGHAAEAEPDLRAALEVTERLFAADGEQVALARTNYGICLARQGRLDEAEVQ
                     FRWVKTTRSRTFGHEHPKTLYDRNGGVCGLHSDELPLADQRRMDHRPGDRRLRRRFPT
                     RHPLPEQLVLPNDIIPADTHSGPTQPLAAVDLRLPHPVTQCLPY"
     gene            complement(2032..2169)
                     /locus_tag="VFB89_14410"
     CDS             complement(2032..2169)
                     /locus_tag="VFB89_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28537.1"
                     /translation="MRKYPDEVRERAVRTVFEIGEKSGQQQGAIAWVADRLGVNRGGT
                     A"
BASE COUNT          402 a          727 c          737 g          396 t
ORIGIN      
        1 tcacactgcg ccgccgatgc aggtctacac caacaaactg ccggccggca tactcctcca
       61 caggggcctc cttcgtcgta gtggaccatg acaatccaaa ggaaacccct gaacggggtg
      121 actgtcacga ggagcgaggc ctcgcccctt tcatcggatc agggcacggc gcgcaacatc
      181 cgcttctgcc gcgatcgggt cgctacacgc ggtgactgcc gcgggtgtga ccgcctggag
      241 gttcgtggtc ggtggcgttc aaaggcgatg cgtaccagcc gtacggtcgg atcatctcat
      301 ctgcgaccct cagcggtggc catcggggcc cgttgaggca ttcggtccct gggtcgctga
      361 cggacagggg gactggtcca atggcccgga tggctggtcc acagccgtcg aagggtggcg
      421 cccacgcggt cctgagagcg ggggtcattc tcggcgtgat gcggcgtctt tgttggtcat
      481 cgacggatct cgggtcttct gccgaggagg agacgcagaa tccgtagggc gcgccggcgg
      541 cgtgcgtggt ctttcgacca caggtacacg ccggcggcca tgcttaggag aaggatgatc
      601 ggggtcgagg cctccaagat gccgatcaat tctgcaagat ttggatcgag gacgtagcgc
      661 gatgccgccg agagttcagt catcgttcaa ggctgatccg ccgagagagt gacttgccgg
      721 ctactgccaa agacgtacgt cagagcctgt cgcagtttgt atcccgggac ggctgggtcc
      781 accttcttgc acgagaggac ggggtcgcgc ggttttacaa tctgtgccag gctcttccgc
      841 tgtaacgcac agtcaaccgg gggaccgctc cgatgtttcc gcaaagatca gaaaacgaat
      901 cgcctgcagc gtcgaggttg ttccagcttc tgcagcggct gcatcttgaa gccggtgccc
      961 cgagcaggcg cgatatctcc aagcgcctcg ggcagggtgt actgagccac acgactgtca
     1021 acggcgttct ccgtcgtccg gcggaggtca agtggggcca gctcgaactg gtcgccgacg
     1081 cgctcggtgg tgagatcgcc gagcttcggc agttgtggac cgcggcccag gaggaacgcg
     1141 ctagtgggat cgtcgcttcc ggacgggctc atgccagcag cgagtcgccc atggtgggcc
     1201 tgttcattgg cgacctgagc gactgggtgg acaaggcaga gcacgcattc agcgcggggg
     1261 actacatctt cgcccagcgg tcggctgccg tggtggcggc cgccgcactc gcggctgaag
     1321 gcgaggcggc ccgccgtctt ggccaccttg aacgcgccga cgcggcgctg cgcgaggcgc
     1381 ttgacctgca gaaggcgaat gttggtccga acgacccaat gacgttgctg acccggcagc
     1441 gcctggcacg cgtcatgttc caacaagggc acctggacaa cgcgctaggg gagataacag
     1501 ccgtcgtcga agcctgggag agacttggcg cacgagacga tcacagaggt ctcagggctc
     1561 gcttggagct tgggttcatc cagtcgatga ccggccacgc cgctgaagcg gaaccggatc
     1621 ttcgtgcggc gcttgaggtg acggaacggc tgttcgcggc cgacggcgag caggtggcgc
     1681 ttgcccgcac caactacggg atctgtctgg cgcgtcaggg ccggctggat gaggccgaag
     1741 tgcagttccg atgggtgaag actactcgaa gccgaacatt cggtcacgag cacccaaaga
     1801 ccttatacga tcgcaacggc ggggtgtgcg gcctgcactc ggacgaactc cccctcgctg
     1861 accaacggcg gatggaccac cgtccgggag atcgccgact gcgccgacgg tttccaacgc
     1921 gccacccgct cccggagcaa ctggtcctgc ccaacgacat aatcccggcc gacacccaca
     1981 gtggacctac ccagccgctg gccgccgtcg acctccgcct gccgcaccca gttacgcagt
     2041 gcctccccta ttgacaccca accggtcggc tacccaggcg atcgcaccct gctgctgccc
     2101 ggacttctcc ccgatctcga acaccgtccg cacggcccgc tcacggacct cgtccgggta
     2161 cttcctcatg ctggcatgac tccgacaact cctaaccttc gcaaggttgg gagtctccaa
     2221 cgaacccggg gcggctcacc gcttgttgcc gggctcggtc tc
//
LOCUS       DASRJQ010000636         3821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_714410, whole genome shotgun sequence.
ACCESSION   DASRJQ010000636 DASRJQ010000000
VERSION     DASRJQ010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714410"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            978..3056
                     /locus_tag="VFB89_14415"
     CDS             978..3056
                     /locus_tag="VFB89_14415"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM28538.1"
                     /translation="MTPQCDRRIACQTSSDLAGVAANSSFRKRKGRQASEFHAPLADS
                     KHPSPRPVAVADLAGPLGDALLNRYVVERELGRGGMATVFLARDLKHDRPVALKLLRP
                     ELAAILGAERFLREIRLTANLQHPHILPLIDSGDAAGQLFYVMPYVEGESLRQRLQSE
                     VQLPIGEALRLTTEIAEALDYAHQQGVIHRDIKPENILLSRGHALVADFGIALAVTQA
                     GGDRLTETGLSLGTPAYISPEQAMAESRLDGRSDQYSLACVLYEMLAGEPPFTGPTAQ
                     AIIAKRFQAAAPSVRQIRERVPESVDRAIGRALARTPADRFATGEEFARALERGSQEV
                     TLVRGQAVGQQLPGETLGFAKPRTKWPAGRVVALLSACALVVIGWTVVRRLTSRGPAL
                     SPTRVAVLPFSTSGSSNVVYLAEGMVDLLSRNLNGAADLRTIDPGTVLTAASKAGRRA
                     NAEEGRALARRLGAGLYVLGSVHANGSRLRIQAELYGEADTAEGGLARAAAEGDSTQL
                     FQLVDRVSADLLAGRGRGLGSRLMQSAALTTGSLPALKDYLEGERNLRGGLGNFDSAI
                     AGFQRAVAKDSTFALAYYRLGVAGGWAERPGISGPATQRALLLSGRLDDRDRRLLSAF
                     AALQRGAADEAEQQYRTILRDYPDDLEAEFQLADLLHHYNAPRGRPMAEAREPFDHVL
                     ELDPGFLCPI"
     gene            3060..>3821
                     /locus_tag="VFB89_14420"
     CDS             3060..>3821
                     /locus_tag="VFB89_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28539.1"
                     /translation="MWDLAVREEDYARADSLLRRKFTADKRPLEHRAMLALVQRDSGA
                     LARLLDEVRKKSSGYPSAPASIALYLNDFRAADEFATAALASPRPQAIRDSLHQLLAV
                     LALAQGSWAAAKREFALVERVMPSARRQHALSATRPFLPVPRSDIALLRDELEKWDPS
                     ADPREPNPGLASALHPHLRLYLLGVLSSRLGEDALALQHADELQRIDRPPEVATLVRD
                     LALTVRAEVSARRGRPAEALKLLEPIKGEVPPELLA"
BASE COUNT          664 a         1252 c         1221 g          684 t
ORIGIN      
        1 gagggagctg ttcgattcgc tcactagttc caagtccacc cagattgtcc cacgcaccgg
       61 gtggccgcag cacgttccca aaatggaagg caaggggaac caggttgtgc aggtttggtc
      121 gtgggtgcca agggaaggtg agggggtgat cgaggtaatt cggcaggcct cgacctattg
      181 gaagcgggac aggtggtact taatcaaaac gactgacggc cgtatacgga tcagccggac
      241 gcccgacaag agcattgagg gcgaccccca ccacgctgat aagctcccag gttggtcact
      301 cgaccggccc tgatggttag tacaccttcc agtgcacctg tgatggcgcc ttttcgtagc
      361 gcacggtggc gcgtcatggc gcattctcgc tgtaagagcc cggtttctcc tctgggggat
      421 tcgcactcat aatgccgggg tcctaggtcc tccgaacggc ggcagtcaaa tcgcgcgtgc
      481 ttgtgtaact gatagtgtaa ctaatcaacc ccaatcaccg acaaacatcg ccaacaacag
      541 ggttgccacg aaaatcttca agacgctcag ggcaacgact tacggctgga agtaggtggt
      601 ggtaattgag cctcgtggtg aatgcgcagg gctcaaaatc caccgggggc aaccccgtgc
      661 gagttcgact ctcgcctcgg gcactccgct ctagctaacg acttcgggcc tccaggttca
      721 accgatccgt gaggcccgtt tcatcggata ctctgccgtt tgtctgccat gaccgtcgct
      781 ctctgagtgg accggcctcg gctgttcggg gaaggtggtc ggttgatcgc ctaaaaccgc
      841 cagcgaaccg tagaattcgg aactgcgagc ctgtcggccc gcagcagaag tggcggtcat
      901 gaggttggat gctatgactg catcttggaa ggcgtttcaa gacctggtag cgagaatcga
      961 gaagatcgcg tccggtcgtg acgccacagt gcgatcgccg gatcgcgtgc cagacgtcga
     1021 gcgatctggc gggtgtggcg gccaactcca gcttccggaa gcgcaaggga cgtcaggcta
     1081 gcgagttcca cgcccctcta gcagattcga agcatccttc gccccgaccc gttgcggtgg
     1141 ccgacctcgc aggtccgcta ggagatgcgc tccttaatcg ctatgtcgtc gagcgcgagc
     1201 tcgggcgagg cgggatggct acggtctttc tcgcccgcga cctgaagcat gatcgccccg
     1261 ttgcgctcaa gctcctccga cccgagctgg cagccatcct cggcgccgag cgcttcctgc
     1321 gagagatccg tctcacagcc aacctgcagc atccccatat cctcccgctg atcgattccg
     1381 gcgacgcagc gggtcagctc ttctatgtga tgccgtacgt cgagggcgag tcactgcgcc
     1441 agcggctcca gagtgaggtc cagctcccga tcggcgaggc gctccggctc acaaccgaga
     1501 tcgccgaggc actcgactac gcccaccagc agggagtcat tcaccgcgac atcaaacccg
     1561 agaacatcct gctctcgcgc ggccatgccc tggtcgcgga cttcgggatc gcgctggcgg
     1621 tcacgcaggc cggcggggac cggctgaccg aaacgggcct ctcgctgggg acaccagcgt
     1681 acatcagtcc ggagcaggcg atggccgagt cgcgtctcga cggcaggagc gaccagtaca
     1741 gcctcgcctg cgtgctgtac gagatgctgg ctggcgagcc gcctttcact ggtcccacgg
     1801 ctcaagcaat catcgccaag cgcttccagg cggccgctcc ctccgttcgg cagatacgtg
     1861 agcgtgtccc cgagtcggtc gaccgcgcga ttggccgggc gctggccagg accccagctg
     1921 atcgtttcgc caccggggag gagttcgccc gggctctgga gcgggggagt caggaggtca
     1981 cactggtccg tgggcaggct gtcggacaac agctacccgg cgagacgctc gggtttgcga
     2041 aaccgcgtac caagtggcct gctggccgtg tggttgccct gctctcggcg tgcgcccttg
     2101 tcgtcattgg ctggacggtc gttcggcggc tgacctcccg cggaccggcc ctctcaccca
     2161 cgcgcgtcgc ggtgctgccc ttttcgacgt ctggcagttc gaatgtcgtg tacttggccg
     2221 aggggatggt ggacctcctc agccgcaatc tgaacggggc ggctgatctc cgcaccatcg
     2281 atcccggcac cgtgctgacg gccgcgtcca aagcgggtag gcgggccaat gcggaggaag
     2341 gtcgggccct ggcgcgacgc ctcggggcgg gactctacgt ccttggcagc gttcatgcca
     2401 acggcagccg gctgcggatt caggcggagc tctacggcga ggccgatacc gcagagggag
     2461 gcctagcgcg ggccgccgca gaaggcgaca gcacgcagct ttttcaacta gtcgatcgcg
     2521 tatcggcaga cctgttggcc gggcgcgggc gcggattggg ctccagactg atgcagagcg
     2581 cggctttgac gacgggttcg ctgcccgcac tcaaagacta tctggagggc gagcgaaacc
     2641 tccgaggtgg gcttggtaat tttgattcgg ccatcgctgg attccagcgc gcggtcgcta
     2701 aggacagcac ctttgccttg gcctactatc ggctcggcgt ggccggtgga tgggctgagc
     2761 gcccgggaat ctccggaccg gcgacccagc gggcgctgct cctgagtggg cgtttggacg
     2821 accgggaccg gcggctcctg tctgccttcg ccgcccttca gcgcggcgcc gcggacgagg
     2881 ccgagcaaca gtaccgcacg atcctgcgcg actacccgga tgacctcgag gccgagtttc
     2941 agctggcaga tttgttacac cactacaacg cacctcgcgg ccggccaatg gcggaggcgc
     3001 gggagccctt cgaccacgtc ctcgagctgg acccaggatt cctctgccca atatgaccga
     3061 tgtgggacct cgccgtgcgc gaggaggact acgcgagagc cgactcgctc ctacgccgaa
     3121 agttcactgc cgataagcgt cccctagagc accgcgcaat gctcgcgctc gtgcagcgcg
     3181 actcgggcgc tctagcgcga ctccttgacg aggtcaggaa gaagtcctct ggttacccgt
     3241 ccgcgccggc atcgatcgcg ctctacctca acgacttcag agcggccgac gagtttgcca
     3301 cagccgctct cgcgtcgccg cgtccgcaag cgattcgcga ctccctccac caattgctcg
     3361 ccgtgttggc actggcccaa ggcagctggg ctgccgcgaa gcgggagttc gccctcgtcg
     3421 aacgagtgat gccatcggcc agacggcaac acgccctcag cgctacccga ccctttctcc
     3481 ccgtgccacg ctcggacatc gctctgctcc gggatgagct ggagaaatgg gacccgagcg
     3541 ccgacccccg cgaacccaat ccaggcctcg cctccgccct gcacccgcac cttcgcctct
     3601 acctcctcgg tgtgctgagc tcccggcttg gtgaggacgc gctagccctc cagcatgcgg
     3661 acgagctgca acggatcgac cgtccgcccg aggttgcgac tcttgttcgc gacctggcgc
     3721 tgaccgttcg ggccgaggtc tcggcgcgcc gggggcgtcc agccgaagcg ctgaagctat
     3781 tggagcctat aaaaggcgag gtcccacctg agctgctcgc c
//
LOCUS       DASRJQ010000637         2881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_4219718, whole genome shotgun sequence.
ACCESSION   DASRJQ010000637 DASRJQ010000000
VERSION     DASRJQ010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4219718"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VFB89_14425"
     CDS             <1..678
                     /locus_tag="VFB89_14425"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZM28540.1"
                     /translation="RRVPLFEGLSDADLNSFADFIRERSYPKGSVIVFEDDPGDALYV
                     VASGQVKVVLVAEDGREVILSVLGPGSFFGEMAILDDEPRSAHVIAMEESNLLMLRRE
                     DFQARLRSMPDVAISLLREISRRLRRADEKIGSLVLLDVHGRLAHLLLRMADEEQGDR
                     ITRKLTHHTIAQMIGSSRETVSRAMRNLVEQNIIEVTRTDIALKNRQALLAAARRERA
                     ATVPRPA"
     gene            704..1567
                     /locus_tag="VFB89_14430"
     CDS             704..1567
                     /locus_tag="VFB89_14430"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZM28541.1"
                     /translation="MGYSLTFWGTRGSIPTPGPRTARYGGNTPCVGVSDDEGHLVVLD
                     AGSGLRPLGSAIMAEGGGMVAHLLLTHTHWDHIQGFPFFKPLGVVGNEVHVYGAAQEA
                     VELSEILRRQMEPTVFPVPLSALAANIVVSPITEGTFEIDGFRVGAFRLRHPGTTLGY
                     RLAPRSGGREVAYVTDNELGPGGTYSVGPNWRTALVEFLGGVDTLIHDAMYSESVIAA
                     RAGWGHSTPRESIDLAADAACKRLILFHHDPECDDETIDQRLREARDYAASRAPHLEV
                     DAACEGERLTL"
     gene            complement(1557..2804)
                     /locus_tag="VFB89_14435"
     CDS             complement(1557..2804)
                     /locus_tag="VFB89_14435"
                     /inference="COORDINATES: protein motif:HMM:NF024779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HZM28542.1"
                     /translation="MRLRGWLAVALLGASAPAAAQSLRPPAPAVAGSELTISLMTMGV
                     GVRVWELFGHNAILVEDRSRGTAVAYNYGMFDFRQENFLWRFIQGRMRYWMEGFDLAS
                     TLEIYRRANRSVWVQELNLSPSQRVALRDFLEWNERPENRYYRYDYYRDNCSTRVRDA
                     LDRIIGGRLRAQTDTIRTGHTYRFHTERLTSSDPLLYTGLLVGLAQPADRRLTAWDEM
                     FLPLELRAWIRHVKVPDAAGGEAPLVSSERVLFEASQPDPPSTPPHWFLGYLVIGLLL
                     GSGLWWLGSHAAGNGWQGKGFVFLAVLWTFITGVSGGMLAFFWAFTDHAVAYRNENLF
                     QANLLAVPLIVLIPALKRKADWAQRPAVGLAVLIALVSLLGVFVKILPAFYQDNVELL
                     ALAVPANLGLAAGVVTAARRLKA"
BASE COUNT          552 a          908 c          932 g          489 t
ORIGIN      
        1 cgccgagttc cgctgttcga gggcctctcg gacgcggacc tcaactcgtt tgctgatttc
       61 atcagggaac gaagctatcc caaggggagc gtcatcgttt tcgaagacga cccaggtgat
      121 gcgctgtatg tggttgcgtc cggccaggtc aaggttgtgc tggtggcgga agatggtcgc
      181 gaggtaatcc tctccgtact gggtccggga agctttttcg gggagatggc gattctggac
      241 gacgaacccc gctcggccca cgtcatcgcc atggaagaat ccaacctcct catgctgcgc
      301 cgcgaggact tccaggcccg gcttcgctcg atgccggatg tcgccatctc cctgctacgg
      361 gagatttccc gccggctccg gcgggcagac gaaaagatcg gcagtctcgt cctgctcgat
      421 gtccacggtc ggctggccca cctcctcctg cgcatggccg atgaagagca gggcgatcgt
      481 attacccgaa agctcacgca tcacacgatt gcacagatga tcggctcgag ccgggaaacg
      541 gtgtcgcgtg cgatgcgcaa tctggtagag cagaacatca tcgaagtgac ccgaaccgac
      601 attgcactca agaatcggca ggccctcctt gcggcggcgc ggcgagagcg cgcagctact
      661 gtcccacggc ccgcgtgagg ccgaggcgcc ccgcaccaag tctgtggggt acagcctgac
      721 cttttggggt acccgcgggt cgattccgac cccgggaccc cgaacggcgc gctatggtgg
      781 caacacgccc tgcgtcggtg tttcggacga cgaaggacat ctggtcgtat tggatgcggg
      841 cagcgggttg cggccgctgg gaagcgctat catggccgaa ggcggcggca tggtcgccca
      901 cctcctgctg acgcacaccc attgggacca catccaggga ttccccttct tcaaaccgct
      961 gggcgtcgtg ggaaacgagg tccacgtgta cggcgcggct caggaggctg tggagttatc
     1021 ggagattctc cggcgacaga tggagcctac cgtctttccc gttccgctca gcgccctcgc
     1081 agccaacatc gtggtcagcc ccatcacgga gggcactttc gagatcgacg gattccgcgt
     1141 gggggcgttc cggcttcggc atcccggcac caccctcggg tatcgcctcg caccccggag
     1201 cggcggccgg gaagtcgcct acgtaacgga caatgagctg ggccctggag gcacctactc
     1261 ggttgggccg aactggcgca ctgcgctcgt agagttcctg ggaggtgtgg acacattgat
     1321 ccacgacgcg atgtactccg agtcggtcat tgcggcccgg gcaggttggg gtcattccac
     1381 cccgcgcgag tcgatcgacc tcgcagcgga cgccgcgtgc aagcgtttga ttctctttca
     1441 ccacgacccg gagtgcgacg acgagaccat cgatcagcgg ctgcgcgaag ccagggacta
     1501 cgcggcgtcg cgagctccgc atctggaagt ggacgcagcc tgtgaaggag agcgactcac
     1561 gctttgaggc gtcgggcggc agttacgacc ccggcggcaa gccccaggtt tgcgggtacc
     1621 gccagcgcca atagctccac gttgtcctgg tagaaagccg gcaggatctt cacgaacacc
     1681 ccgagcagcg aaacgagcgc gatcagcacg gccaatccca cggccgggcg ctgggcccag
     1741 tctgctttcc gcttcagtgc cggaatcagc acgatgagcg ggaccgcaag cagattcgcc
     1801 tggaagagat tctcattgcg gtaggcgacg gcgtggtcgg tgaaagccca gaaaaaagcg
     1861 agcatcccac ctgaaacgcc ggtgatgaac gtccagagca cggccaggaa gacgaatcct
     1921 ttgccctgcc aaccattccc ggccgcatgt gaccccagcc accagaggcc agagccgagc
     1981 aggaggccga tcaccagata gccgagaaac caatgaggtg gcgtggacgg cgggtccggc
     2041 tgagacgcct cgaacagcac acgctcgcta ctcaccagcg gcgcttcgcc acccgctgcg
     2101 tcggggactt tgacgtgacg aatccaggcg cgcaactcga gcggcagaaa catctcgtcc
     2161 caggcggtca acctgcgatc ggccggctga gccagcccga ccaggagacc agtgtaaaga
     2221 agtgggtccg agctggtcag ccgctcggtg tgaaaccggt aggtgtgtcc tgtgcgaatc
     2281 gtgtcggtct gagcccggag gcgaccaccg atgatccggt cgagtgcatc gcgcactcgg
     2341 gtggagcagt tgtctctgta gtagtcgtac cggtagtagc gattctccgg ccgctcgttc
     2401 cattccagaa aatcacgcag agcgactctt tgggatggcg agaggttcag ctcctgcacc
     2461 cagaccgagc gattggcccg ccggtagatc tcgagcgtgg acgcgagatc gaacccctcc
     2521 atccaatatc gcattcggcc ctggatgaac cgccagagaa agttctcctg ccggaagtcg
     2581 aacatgccgt agttgtaggc gacggcggtc ccgcgggacc ggtcttcaac caggatggca
     2641 ttgtgtccga acagttccca cacccggaca ccgacaccca tggtcataag tgagatggta
     2701 agctctgagc ccgcgaccgc gggcgcggga gggcgcagcg actgagcagc cgcgggcgcg
     2761 ctggctccga ggagggcgac tgcgagccag ccgcggagcc tcacaaggcc acgtcgcggc
     2821 cgtcctgcgc ggaccgatag acggcgtcga gcaccttatg cagcgtgatc tgctcgtcga
     2881 g
//
LOCUS       DASRJQ010000638         3824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_672283, whole genome shotgun sequence.
ACCESSION   DASRJQ010000638 DASRJQ010000000
VERSION     DASRJQ010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672283"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..442)
                     /gene="rplS"
                     /locus_tag="VFB89_14440"
     CDS             complement(95..442)
                     /gene="rplS"
                     /locus_tag="VFB89_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004415194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HZM28543.1"
                     /translation="MERLAEVAREGLRSDIPAFEPGDTVRVMVRVKEGEKERLQAFEG
                     LVVAKRGGGISENFTVRKVSAGVGVERIFPLHSPTIASIDLVRRGRVRRAKLYYLRRL
                     SGKAARIREKREG"
     gene            complement(485..1180)
                     /gene="trmD"
                     /locus_tag="VFB89_14445"
     CDS             complement(485..1180)
                     /gene="trmD"
                     /locus_tag="VFB89_14445"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HZM28544.1"
                     /translation="MLTIDVVTLFPEVIEPFVATSIPRRAADAGLVAFQVVQLRDFTH
                     DRHRTVDDYAYGGGAGMVLKPEPFFEAVDRLREQGPLGPIVLLSARGRPFRHDDAVRC
                     SLGRRLTLLCGHYKDVDQRVAEGLATEELSVGDFVLSGGEPAALCVLDAVVRLLPGAL
                     SDHESASTDSHYEGLLSPPSYTRPPEYRGLSVPEVLRSGNHAEILAWRQEQAETLTRE
                     RRPDLWAQYLEGR"
     gene            complement(1208..1741)
                     /gene="rimM"
                     /locus_tag="VFB89_14450"
     CDS             complement(1208..1741)
                     /gene="rimM"
                     /locus_tag="VFB89_14450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HZM28545.1"
                     /translation="MTAEAPRHVAVARLRKPHGLKGDLVVFPLTNDPSTAFAPGRSLW
                     VVDLAGTVTGGPLVIERSRAYHREWLLKFRGLENREALEPWRGNFLALPADQLAPPVD
                     DEVYLHELEGFAVQLEDGTAVGLVSALYELPAGLTLEVQGPRREFLLPYRKEFVRGVD
                     REARRLTVTLPDGLLDL"
     gene            complement(1738..2001)
                     /gene="rpsP"
                     /locus_tag="VFB89_14455"
     CDS             complement(1738..2001)
                     /gene="rpsP"
                     /locus_tag="VFB89_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HZM28546.1"
                     /translation="MATRIRLRRIGRKKLPLYRIVVADKESPRDGRFIEVLGTYDPKG
                     ATAADKVRLDAEKARQWLAKGATASDTVQALLKQAGVVASPTA"
     gene            complement(2026..3366)
                     /gene="ffh"
                     /locus_tag="VFB89_14460"
     CDS             complement(2026..3366)
                     /gene="ffh"
                     /locus_tag="VFB89_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HZM28547.1"
                     /translation="MFDQLSDQLNTALSKLTGRGVLTEDAVKEGLREIRAVLLEADVS
                     FELTREFLERVQSKAVGVSAIKDVRPGHQLVKIVYDELVALLGERQAPLTFASVPPTV
                     ILMVGLQGSGKTTTAGKLANRLKGEQKAPFLVAADVYRPAAQEQLATLAKQVDVGVHT
                     EPDAKDVVKLVKHGIEAANKARARTVIVDTAGRLQIDDEMMNELKQLKSAVRPQEILL
                     VADGMTGQDAVRIAKGFHDALGITGVVLTKMDGDARGGAALSIYGVTKAPIKYVGVGE
                     RVDALEPFYPDRMAGRILQQGDILSLVEKAQTTIDEKEAERLAKKVKSKKGLDLEDFL
                     GAMKQMQKMGPLKNVLGMLPGINPAMLQAAKLDEKKLKHVEAIVLSMTPKERSDPEIL
                     TGQRRLRIAKGSGRTVQEVNQLLAQFKQMQKMMKIAGKTGGSLPRLPFGPRFPN"
     gene            complement(3396..>3824)
                     /locus_tag="VFB89_14465"
     CDS             complement(3396..>3824)
                     /locus_tag="VFB89_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28548.1"
                     /translation="ILHPERYTAGDQPIELAFADSVGRVRIEDVLGELDVHLLEAELA
                     GAKTLGKIGVLGWGGDRYRVYDTPAGLALVWYIVWDDSVAATAFHAGTGARLGARQLR
                     GWRGRLDRLVIDDRPATRYVWAPEQWDRWGNVPEATLRLP"
BASE COUNT          646 a         1372 c         1132 g          674 t
ORIGIN      
        1 cgcggccgac ttcatccacg ccgacgagga gccgcccctc gcgccacgcg gcccgctccc
       61 gggtcagcgt cggcacccga agggccggca tcgctcagcc ctcgcgcttc tcccggatcc
      121 tggcggcctt acccgagagc cgcctgagat agtagagctt ggcccgccgg acccggcctc
      181 gacggaccag atcgatcgag gcaatggtgg ggctgtggag cgggaagatg cgctcgacgc
      241 ccacacccgc cgacaccttg cgaaccgtga agttctcgct gatgccgccg cctcgcttgg
      301 cgaccaccag gccctcgaat gcctgcagcc gctccttctc gccttccttc acgcgtacca
      361 tgactcggac ggtgtccccc ggctcgaacg cggggatgtc cgagcgcaag ccctcacggg
      421 cgacctctgc caaccgctcc atcagcgaac cctccgcgcc gtccggcgcc tggtgtggta
      481 cttactaccg tccttcaaga tactgcgccc acagatcggg acgtcgttct cgcgtcagtg
      541 tctcggcctg ttcctgccgc caggccaaga tctcggcatg attgccggaa cgcaggactt
      601 ccggcacact gagccctcgg tattccggcg gccgagtgta actcggcggg ctcagcaatc
      661 cttcgtaatg cgaatcggtg ctcgccgact cgtgatcgct cagcgcaccg ggtaacagcc
      721 ggactactgc atccagcacg cacaaagcgg ccggttcacc gcccgagagg acgaagtcac
      781 caaccgatag ctcttcggtg gccaggccct ccgccacccg ctggtcaacg tccttgtagt
      841 gcccgcaaag cagagtaagc cgccgaccca gcgaacatcg cactgcatca tcgtgacgga
      901 acgggcgtcc ccgggccgac aacagcacga tcggtcccag aggtccctgc tcccgcagcc
      961 ggtcgaccgc ctcgaagaac ggctccggct tgagcaccat cccggcgccg ccgccgtacg
     1021 cgtaatcgtc caccgtgcgg tgacgatcgt gcgtgaagtc gcgcagctga accacctgga
     1081 acgcgaccag ccctgcgtcg gcagcccgcc gggggatgct ggtggcgacg aacggctcga
     1141 tcacctcggg aaacaaggtc acgacgtcga tcgtcagcat cgaatcaccc gccggccggc
     1201 gttcctccta cagatcgagc agcccgtccg gcaaggtgac cgtgagccgc cgcgcttcgc
     1261 gatccactcc ccgcacgaac tcctttcggt acggcagcag aaactcgcga cgcggtccct
     1321 gcacttcgag cgtcagcccc gccggcagct cgtagagcgc cgagaccagg ccgaccgccg
     1381 ttccgtcttc caactgcacc gcgaaccctt cgagttcgtg gagatagacc tcgtcatcaa
     1441 ccggcggcgc cagctggtct gccggcaaag cgagaaagtt tccccgccac ggctccagcg
     1501 cttcgcgatt ctccagtccg cgaaacttga gcaaccattc ccgatgatat gccctgctcc
     1561 gctcgatcac cagcggaccg ccggtgaccg tccctgccag atccactacc cagagcgacc
     1621 gccccggtgc gaacgcggtg ctcggatcat tggtgagcgg aaagactacc agatctccct
     1681 tgagaccatg cggctttcgc agccgcgcca ctgcgacgtg gcgcggcgcc tccgcggtca
     1741 cgccgtcggg ctggcgacga ctccggcctg cttgagcagc gcctggaccg tgtcggaagc
     1801 cgtggcgcct ttcgccagcc actgacgcgc cttctccgca tcgagccgaa ctttgtccgc
     1861 ggcggtcgcc cccttcggat cataggtgcc gagcacctcg atgaaccgcc cgtcacgggg
     1921 cgactccttg tcggcgacca cgatgcggta caatgggagc ttcttgcgac cgatccggcg
     1981 caggcgaatg cgtgtagcca tgtcttgagg ttccgagtaa cgggttcagt tggggaagcg
     2041 cggcccgaac ggcagtcggg gcaacgatcc ccccgtcttc cccgcgatct tcatcatctt
     2101 ctgcatctgc ttgaactgcg ccaacagctg attcacttcc tgcaccgtac ggcctgagcc
     2161 cttggcgatc cggaggcgcc gctggccggt cagaatttcc ggatcggagc gctccttggg
     2221 cgtcatcgac agcacgatcg cctcgacgtg cttcagcttt ttctcgtcca gcttggccgc
     2281 ctgcagcatg gccggattga tgcccggcaa cattcccagc acgttcttga gcggccccat
     2341 cttctgcatc tgcttcatcg cgccgaggaa gtcctcgaga tcgaggccct tcttcgactt
     2401 caccttcttg gcaaggcgct cggcctcttt ctcgtcgatc gtggtctggg ctttctccac
     2461 cagcgacagg atgtcgccct gctggagaat gcgtcccgcc atccgatcgg gatagaacgg
     2521 ctcgagcgcg tccacccgct cgcccacgcc gacgtacttg atcggcgcct tggtgactcc
     2581 gtagatcgag agcgccgcgc cgccgcgagc atcgccgtcc attttggtca gaaccacgcc
     2641 ggtgatgccc agtgcgtcgt ggaatccctt cgcgatgcgc acggcatcct gaccggtcat
     2701 gccatcggcg acgagcagga tctcctgcgg cctgacggcg gacttgagct gcttgagctc
     2761 gttcatcatc tcatcgtcga tctgcagccg gccggcagta tccacgatga cggtgcgcgc
     2821 tcgtgccttg ttcgcggcct cgatgccgtg cttgaccagc ttgacgacgt ctttggcgtc
     2881 gggctccgta tggacgccga cgtccacctg cttggcgagc gtcgccagct gctcctgtgc
     2941 cgccgggcgg tagacgtcgg cggcgaccag aaagggggcc ttctgctccc ctttgagccg
     3001 attggccagc ttgccggccg tcgtagtctt gcccgagccc tgaagaccca ccatcagaat
     3061 gacagtcggc ggcaccgacg cgaacgtgag cggcgcctgt cgttctccca ggagcgccac
     3121 cagctcgtcg tacacgatct tcaccagctg gtgccccggc cgcacgtcct tgattgccga
     3181 aacaccgacc gccttgctct ggacccgctc gagaaattca cgggtcagct cgaagctgac
     3241 atccgcttcg agcaggacgg cccggatctc ccgcaaacct tccttgaccg catcctcggt
     3301 aaggacgccg cgcccggtga gcttgctgag cgcggtattg agctggtcgg aaagctggtc
     3361 gaacataagc gcagaagagt agcggcgtga acgagttagg gcaagcggag cgtggcctcg
     3421 ggaacattgc cccaacgatc ccattgctcc ggggcccaga cgtatcgcgt cgcgggtcga
     3481 tcgtcgatga cgagccgatc gaggcggcct cgccaacccc tgagctgacg cgcgccgagc
     3541 ctggcaccgg tccccgcatg gaaagcggtt gccgcgaccg aatcatccca gacgatgtac
     3601 cacacgaggg cgaggccggc cggcgtgtcg tagactcggt agcgatcacc accccacccc
     3661 agtacgccga tctttcccag cgtcttggca ccggcaagct ccgcctcgag cagatgcacg
     3721 tcgagctctc ccagcacatc ttcgattctg acccgtccga ccgaatcggc gaacgccagc
     3781 tcgatcggct gatcccccgc cgtgtatcgc tcaggatgca ggat
//
LOCUS       DASRJQ010000639         4428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3210684, whole genome shotgun sequence.
ACCESSION   DASRJQ010000639 DASRJQ010000000
VERSION     DASRJQ010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3210684"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="VFB89_14470"
     CDS             complement(<1..912)
                     /locus_tag="VFB89_14470"
                     /inference="COORDINATES: protein motif:HMM:NF024930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28549.1"
                     /translation="MAQDAIRGWSLFAFDTEAYTGPIVQMDAGHNATAVIRADGRLFI
                     HGQHGIGFGATSPPPLPPGLTYVDVSLNMDNGVAIRSDGSAVGWGSSLAQHPPPPPPL
                     PPGTSYVQISAGAWHDLALRSDGVVVAWGNNTWGQCNVPTLPPGVSVVKVHAGDSSLL
                     LLSNGTIVAFGQCQWGECNVPPLPPGTTYLDMFGQNIHVLALRSDGALVAWGDNSFGQ
                     CNVPTPPAGTQYVMAAAGNFHSIALRSDGVVVGWGSNGFGQTNPQVVPPGTQVLKLSA
                     GQYHSLALFSDGTVRGWGHSSYFELPLP"
     gene            complement(1007..2662)
                     /locus_tag="VFB89_14475"
     CDS             complement(1007..2662)
                     /locus_tag="VFB89_14475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28550.1"
                     /translation="MTESLSPRLAFDPPSTTVPGYVPAGGSTDNGSGQWDGIVGRLSN
                     DHASPVPTATMREFHAVVGGREQDGLWGIAELPDNRFVVVGGTAVPTTPGSGAATFPF
                     TNTQLDWNSLTNYCVGVVMAFDASPTRLSPPQNLLLRSSAAVGSAGTPRTHVRDVAVN
                     VWPSTVAPPWEHRIGIVGTTDDSTLFNWFTLGSVVPTTPFGSNTDGFVATALDIPGGP
                     PDLLFLGGSFHGDAGNSSYTGIASWNEYVDHFSVVGQAVGSYANQDLLVANYFIDTPH
                     SSQALLPYRNDLVVASPIETPAQVGTRNATQPSVLFPLSGLAYDQWTLGSPAGGGIAV
                     DERARVNLAGSTSAMGYPAVGPLARSYRGGQDAVRTVVDMVPLRVGRTDGTGSQVHNG
                     QPIPAPPSGMTGGTTPTAALLPFGAQTGAAPVPGSVGRMLIDWKGVAPGPTPSPPNPV
                     HWLLIDRLPANSQIGGSVIQLGLPTPPSALTLGIEDWVPFGVIYSAVPTPVPQSLEVP
                     LVLPPPVPGGVTFSVQALTLLSSSLPCSGCGNLVASPAIIFSY"
     gene            complement(2659..3408)
                     /locus_tag="VFB89_14480"
     CDS             complement(2659..3408)
                     /locus_tag="VFB89_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28551.1"
                     /translation="MSTAFSTHDQTTTSGALLTLAEGWADVKTPGWAPPGPVPVWRDG
                     RTYAVGTIDVQTTDSSNNPMFSGYAVRSVDHPATTWPVWTMPGTGNSRQIVIVQCSNP
                     DQSVAWQNFFYGDSGLYPFIQWWYRAINARAISVWPAATEADTRVAICGETYDSVMPR
                     NQFPGLLGWTRTFATIGGMGALTFGMPSGFIAVYDGSGVLLCPTSSSSVKRTASVRSP
                     TSASESKRLRAGCAMSSLTAASPRLASGASP"
     gene            complement(3704..3913)
                     /locus_tag="VFB89_14485"
     CDS             complement(3704..3913)
                     /locus_tag="VFB89_14485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28552.1"
                     /translation="MVGGLELPLRGVLGDPSIDMRIVYMHQECAGTLGLAVRARYVLD
                     FVRQRLLRNSVGHAWPHDTGDSGQG"
BASE COUNT          851 a         1430 c         1434 g          713 t
ORIGIN      
        1 cggaagcggc agctcgaagt acgaactgtg cccccagccc cgtaccgttc catccgagaa
       61 cagtgcaagg ctgtgatact gccccgccga gagcttcagg acctgcgtcc cgggcggcac
      121 cacctgcggg ttggtctgac cgaaaccgtt ggaaccccaa ccgacgacaa caccatccga
      181 acgcagcgcg atgctgtgga agttgcccgc cgccgccatc acgtactgcg tgccagccgg
      241 cggcgtcgga acgttgcact gaccaaagga gttgtcgccc cacgccacga gcgctccgtc
      301 cgagcgcagc gccagcacgt ggatgttctg accgaacatg tccaagtatg tagtgccggg
      361 tggcaagggt ggcacgttgc actcacccca ctggcattgc ccaaacgcca caatcgtgcc
      421 gttcgacaac aacagcagcg aggagtcccc ggcgtgcacc ttcacgaccg aaacgccagg
      481 tggcaaggtg ggcacgttgc attgtcccca ggtgttgttg ccccatgcga cgaccacacc
      541 gtcggaccgc agcgcgaggt cgtgccatgc gcctgcggag atctgcacat agctcgtgcc
      601 gggtggcaac ggaggtggtg ggggcgggtg ttgcgcgagg ctggaacccc aaccgaccgc
      661 ggaaccatcc gatcggatcg ctacaccatt gtccatgttg agcgagacgt cgacgtaggt
      721 gaggcccggc ggcaacggcg gcggcgaggt tgcgccgaaa ccaatgccat gctgaccatg
      781 gatgaacaga cggccgtcgg cgcgaatcac cgccgttgcg ttgtggcccg cgtccatctg
      841 cacgatcggc ccggtgtagg cttctgtgtc aaacgcgaaa agagaccagc cccgaatcgc
      901 gtcctgcgcc actgccaggg ggcaactcag tacgagtgcc atcgctatca gaccggggcg
      961 cggattcgtg gtgatcatgg cgggtccttg cggaacgcga tctccttcag tacgagaaga
     1021 tgattgccgg gctggcaacg aggttcccac agcccgagca cggaagggag ctgctcaaca
     1081 gagtgagcgc ctggaccgaa aacgtcactc cgcctgggac tggaggcggc agcaccaacg
     1141 ggacctccag cgattgaggc acgggggtgg gcaccgcact gtaaatgacg ccgaagggca
     1201 cccagtcctc gatgcccagg gtcagcgcag aaggcggcgt ggggaggccc aactggatca
     1261 ccgatccgcc aatctgcgag tttgcgggca gtcgatctat gaggagccag tgtaccgggt
     1321 ttggcgggct cggcgttggt cccggcgcca cgcccttcca gtcgatcagc atgcggccga
     1381 cggatccagg aaccggcgcg gctccagtct gcgcgccgaa cggcagcaac gctgcggtcg
     1441 gggtagtgcc tcccgtcatt ccggatggcg gcgccggaat cggttgccca ttgtgcacct
     1501 ggctgccagt cccgtccgtt cgccctaccc gcaatggtac catgtcaacc accgtccgca
     1561 ccgcgtcctg gccgcccctg tacgatcgcg ccaggggccc cacggcggga taccccatcg
     1621 ccgaagtcga gcccgcgagg ttgactcgcg cgcgttcatc cacagcgatg ccaccacccg
     1681 cgggggaccc gagcgtccac tggtcgtaag ccagccctga taggggaaag aggacggaag
     1741 gttgcgtcgc gttgcgagtt cccacctgcg ctggagtctc gataggggac gcaacaacca
     1801 agtcattgcg ataagggaga agcgcctgac tcgagtgagg agtgtcgatg aagtagttcg
     1861 caacgagcag atcctggttg gcataagaac caacagcctg tcccacgacg ctgaagtgat
     1921 cgacgtactc gttccacgaa gcgatccctg tgtagctgct gtttccggca tcgccatgga
     1981 agctcccgcc gaggaacaac aggtcgggtg gcccacccgg gatgtccagg gcggtagcca
     2041 caaagccatc ggtgttggaa ccgaatgggg tggttggaac aacggagcct aatgtgaacc
     2101 aattgaacag ggtgctgtcg tccgtcgttc ctacgattcc gatccggtgt tcccagggcg
     2161 gagcaacggt cgaaggccat acgttcaccg cgacatcgcg cacgtgcgtg cgcggcgttc
     2221 ccgcgctgcc gacggcggcc gaactcctga gcaggagatt ctgaggcggc gacaacctcg
     2281 tcggtgaggc atcgaaggcc atgacgacgc ctacgcagta gttggtgaga ctgttccagt
     2341 ccaattgggt gttggtgaag gggaaagtcg ctgcaccaga ccctggagtc gtggggactg
     2401 cggtgccacc aacgaccaca aagcgattgt caggaagttc ggcgattccc cacaagccat
     2461 cctgctctcg tccgccgacg accgcatgaa attctcgcat agtcgcggtc ggaaccgggc
     2521 tggcatgatc gttcgacaga cggccaacga ttccgtccca ctgcccagag ccgttgtccg
     2581 tggaaccacc cgcagggacg tagccgggaa cggtggtcga tggggggtcg aacgccaaac
     2641 ggggactcag cgattccgtc acggcgaggc tccggacgcc aaacgaggag atgccgcagt
     2701 aagtgacgac atcgcgcagc ccgctcggag acgcttcgac tcggatgctg acgtcggtga
     2761 tcgcacactg gccgtcctct tgactgaaga agaactggta ggacagagca gcacgcccga
     2821 gccatcgtag acagcgatga atccgctcgg catgccgaag gtcaaggcgc ccatcccgcc
     2881 gatcgtcgcg aacgtacgcg tccagccaag caaaccaggg aactgattcc ggggcatcac
     2941 ggagtcatag gtttcgccgc agatcgcgac gcgggtgtcc gcttccgtgg ccgcgggcca
     3001 gaccgagatg gcgcgcgcat tgatggcgcg ataccaccac tgaatgaacg ggtagagccc
     3061 cgagtccccg tagaagaagt tctgccaggc gaccgattgg tcggggttcg aacactgcac
     3121 gatgacgatc tgccggctgt ttcccgtgcc tggcatcgtc cacacgggcc atgtagtggc
     3181 cgggtggtca acgctgcgaa cggcgtagcc cgagaacatc ggattgttcg acgaatccgt
     3241 cgtctgtacg tcgatcgtgc cgacggcgta ggtgcgccca tctcgccaga ccggcacagg
     3301 ccccggtggg gcccaccctg gcgtcttcac gtcggcccag ccttccgcca gggtcagcaa
     3361 agcgcccgat gtcgtcgttt gatcatgcgt ggagaacgcc gtggacacct gcgtggtgcc
     3421 tgatgcccca gagggcggtt ggttctcctg tgcggtcagg gacacagagg gacacaagac
     3481 gagtacaaag ccacatgcga ggtgagcgat gaggttcatg gcttgtcctt gctcccggca
     3541 gatccactct gccttgcatt cgacccgacg cactcagggt gcaccgcatc gtgcaaaccc
     3601 agcaggtggc caccgacgaa ccgtgacaac gtacgcttct cggaatctgg ggcaaccccc
     3661 ttgggcctgc ccgcctgccc gcctgcccgc tacccgagat cgatcatccc tgcccgctgt
     3721 cgccagtgtc gtgaggccag gcatgaccga cggagttccg gagcagccgc tgccgcacga
     3781 agtccagcac gtagcgggcg cgcactgcca accccagcgt gccggcgcac tcctggtgca
     3841 tgtagacaat gcgcatgtcg atgctcggat cgccgagcac gccgcgtagc ggtaactcga
     3901 gaccgccgac cacgaacagg cagcggcacc tcgacggtct cgacttcgcc gccgccaaac
     3961 gccaccgcct tgcgcgtgcg gccggactcg atgtcgatgt cgacgagttc gacctgcccg
     4021 gcacgccaaa gcgccgatcc gtggacctgc gccagcggcg gcacccgatg ccagtatcgg
     4081 cgcatgaagt ccgaggtcat ctggccgaga cgctacggac cggcccgcgg ctcgcaagcc
     4141 ctggacgcct gcttccaccg acgttggacg cacgacgtcg cgacccatga ctgccgcggc
     4201 agggtgcagg atcaccttgc gttcgatgac ccgagcctgc gatcgaggtg atcgtcggcg
     4261 gctgacgcag cgggactcga aaggctactt gcccgcgagg cgcttcttca cgcgcgcgcg
     4321 gaacgtgtcg aggatcgcct tgccgaggtc gctggagttc gcgacccact cggccttgct
     4381 gaaggcgcgc gggctcgtcg cggcgcggat cgccgcgagg tagggctc
//
LOCUS       DASRJQ010000640         6736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1414125, whole genome shotgun sequence.
ACCESSION   DASRJQ010000640 DASRJQ010000000
VERSION     DASRJQ010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6736
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414125"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="VFB89_14490"
     CDS             complement(<1..847)
                     /locus_tag="VFB89_14490"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZM28553.1"
                     /translation="MTLVGSADAVDVSVLVPAKDEAANLPEFLQLCAAALVPAGFSYE
                     VIVVDDGSRDETPRVLRELKATYPFLRVVTHRRQRGIADALRSAGDAARGDIFVFYPA
                     DLQYLPEDIPALVRPILEGRADIVTGTKQGKYEKAFVSSIYNRLCRALFGVRVTDLNS
                     VKAYRREVIADVPLRPDWHRFMVVIAAADGFRLTSHPVALHPRKAGVSKFTWRRIPVG
                     VLDLLSVWFQLRFGRKPMLFFGVLGATLFALGFLAGVVALVVRFGFGIGFRPLLNLVE
                     TMVISG"
     gene            complement(844..2367)
                     /gene="rpoN"
                     /locus_tag="VFB89_14495"
     CDS             complement(844..2367)
                     /gene="rpoN"
                     /locus_tag="VFB89_14495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HZM28554.1"
                     /translation="MSLRTGLSQQIGLRQELRVNPRLYQAMDMLYMPMMDLQQHLKQE
                     LLANPFLELLEPEDEESADQEKQQEQDKEKEKEQQQKDDEMDWEEILLNGFDVGGTRE
                     QFEVQEYTEPVTVETRNLTDHLREQLQLMELTPRQRLLAEEFLGNMTEDGYLAASLEE
                     LVGSVNQMVATHFERPNEPEDALDEMVDEAALPSPASGAALYTLAEAEEMLAIVQKLD
                     PPGVGARDLRECLLIQLREAGDTESLTYRLVAEAFPDLIAHRWNDLAKRFGVEPGGVQ
                     SAADQLARFDPKPGLKHSERSDGYIIPDLIVEKIADRYHVFLNDTGMPRLRLSRAYQE
                     VARDKKKLTQENREFIASKMNSANWMIQAIEQRRQTMLKVMNFIVDRQRDFFEKGVEY
                     LKPLTLREVAEVINMHESTVSRVTNEKYVQTPRGVLPLKFFFSSALATASGEDASARS
                     IRAKLQKMVGEENPSKPLTDQQIVHLFQEQGIQIARRTVAKYRDQLGILPARMRKRV"
     gene            complement(2364..3509)
                     /gene="lptB"
                     /locus_tag="VFB89_14500"
     CDS             complement(2364..3509)
                     /gene="lptB"
                     /locus_tag="VFB89_14500"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HZM28555.1"
                     /translation="MNQPLWLAQFDGADPSLPLAVASLVAAADEDGRSSLTRLLLAYR
                     EGYLRVYRPAEGLGESVRAHVTTSVLPRLALDGWIEDTRPEWEEVRAAGAWWSDAVKD
                     RDMVGAALLGVAHRARDLLAVEPPASSPSGSVLEGLDLTKSYRGRRVVDQVCLRLEQG
                     EIVGLLGPNGAGKTTTFYLLTGLIRPEAGRVLLDGTDLTRAAMFRRARIGIGYLAQEP
                     SVFRRMTVEENILAILETRKSNRTERQRSLERMLDELGIRHLRKVKAYALSGGERRRL
                     EITRALVTEPRFMLLDEPFAGVDPIAVHDIQTIVAGLRHRGIGVLITDHNVEQTLDIV
                     DRAYIMFEGKVQAAGTVRELVYDERVAERYLGPTLTTRLRARLEHAA"
     gene            complement(3506..4849)
                     /locus_tag="VFB89_14505"
     CDS             complement(3506..4849)
                     /locus_tag="VFB89_14505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28556.1"
                     /translation="MRWLAVSIVVAAAAGPLEGQTSDRCTLQIDNVDRQGIAVETSGG
                     INYFAGGNVRLSCRGTKITMFSDSVASYAGKTVVFNGHVRYRDTTLAMDTDFGTYYRS
                     DERWEARRNVKTRNTKTGSTLTGPSLDYYRAVKGVRDTTEMYAVSRPRIEYYAKDSTR
                     GDSAATDSTGKPAEPYVIVADRVRIKGDDRIWGGGKVTVDRSDFAARGDSLRLDTGPA
                     NDGTLLGSRPELHGLGSDSFDLSGRRLDFRLKNRDLDRVVAKGDSKAVNKDWTLVADT
                     IVHDLVDRKLVHTEAWGDTIRPYAKSAKYEIKADSIALDSPDQQLKEARAFGKAWLGG
                     QVDSLTKERDWLTGDTVIATFAVHDSAGSSKAVLTKLVARGSARSYHLESNEKKKPGE
                     RPSINYARGDEIVITMKSDGSEAVDLVQIRGKADGVQLEPSEEKAAARDTTRAQP"
     gene            complement(4846..5421)
                     /gene="lptC"
                     /locus_tag="VFB89_14510"
     CDS             complement(4846..5421)
                     /gene="lptC"
                     /locus_tag="VFB89_14510"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HZM28557.1"
                     /translation="MRRASRGPVRLGVILVVAATCGSVVACESGGARPTATVQAADTA
                     DQVLEGFSHYVTENGVRKSHIEADTAYFYEASQLALLRKLRATFYDAKGAESSKLTAD
                     HGTYRWQDGSMQADGKVVVIATDGRKLETETLKFDPKKNEISTDQAFRFLHDNEFIQG
                     TSFSSDPDFKNVVTNRPHGVAGKGVLLPGQQ"
     gene            complement(5421..>6736)
                     /locus_tag="VFB89_14515"
     CDS             complement(5421..>6736)
                     /locus_tag="VFB89_14515"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HZM28558.1"
                     /translation="TVQVIPHITDEIKNAIRRTAPGHDVVITEIGGTVGDIESLPFLE
                     AIRQFRQEVGRDNALFIHLTLVPFIAAAGELKTKPTQHSVRELMQIGIMPDILVCRSE
                     QPISADIKRKIALFCNVDFDCVVESPDVRSIYEIPLRFHEQGLDRVVSERLRLTTAEP
                     DLKPWKEITQRILNPSHRVRIAVVGKYTDLHDAYKSVQEALIHGGIAHDAGVEIHWIS
                     SDQLTDTETAGRVLENFDGLLIPGGFGIRGVEGMIEAIRWAREHDLPFFGICLGLQVA
                     IIEFARDVCRLPDSNSTEFAPECESPVISLMPSQRDVSNLGGSMRLGAYTAKLRPGSK
                     VAQAYGAGEISERHRHRWEVSNAYRDVLAEYGLRLSGQSPDGGLVETIELPDHPWFIG
                     CQFHPELKSRPTRPHPLFVGFIGAALKRRRGGAGVEAKELATVAR"
BASE COUNT         1165 a         2203 c         2074 g         1294 t
ORIGIN      
        1 taccgctgat caccatcgtc tcgacgagat tgagcagagg acggaatcca ataccaaaac
       61 cgaagcgaac caccagcgcc accacacccg cgaggaaacc gagcgcgaac agcgtcgctc
      121 cgagcacccc gaagaacagc atcggctttc ttccgaagcg gagttggaac cacacagaga
      181 gaagatcgag cacaccaacc gggatgcggc gccaggtgaa tttcgatacc cctgcctttc
      241 gtgggtgcaa ggcgacagga tgcgaggtca ggcgaaagcc gtccgctgcg gcaatgacga
      301 ccatgaatcg gtgccagtcg ggtcgcaggg gaacgtcggc gatgacctcg cgccgatacg
      361 ccttgaccga gttgaggtcc gtgacccgca caccgaacaa tgcgcggcat aagcgattgt
      421 agatgctgga cacgaacgcc ttctcgtact tcccctgctt ggtgccggtg acgatgtcgg
      481 cccgtccttc caggatgggg cgcaccaggg cggggatgtc ttccggcaga tactggaggt
      541 cagcggggta gaacacgaag atgtcgcccc gggccgcatc acccgcggaa cgcagggcgt
      601 cggcgatgcc gcgctgacgt cggtgagtga cgactcggag aaacgggtag gtcgccttga
      661 gctcccggag cacccgcggc gtctcgtcgc gggagccatc atccaccaca atgacttcat
      721 agctgaaccc agccggtacc agggccgcgg cacagagctg aagaaactcc gggagattag
      781 cagcctcgtc cttggcaggc acgagcacgc tgacatcaac ggcgtcagcg gagccgacca
      841 gggtcatacg cgcttccgca tcctcgccgg cagaatgccc agctggtcgc gatacttggc
      901 gaccgtgcgg cgagcgatct gaatcccctg ttcctggaag aggtggacga tctgctgatc
      961 ggtgagcggc ttggacgggt tctcctcgcc gaccatcttc tgcagcttgg cacgaatgga
     1021 gcgggcgctc gcgtcttcac cggaagcggt cgcgagtgcg ctggagaaga agaacttgag
     1081 cgggagcacg ccccggggcg tctgcacgta tttctcgttg gtcaccctac tcacggtgct
     1141 ctcgtgcatg ttgatgactt cggcgacctc gcgtagcgtg agcggcttca ggtactcgac
     1201 gcccttctcg aagaaatcgc gctgccggtc cacgatgaag ttcatgacct tgagcatggt
     1261 ctggcggcgc tgctcgatgg cctgaatcat ccagttcgcg ctgttcatct tcgacgcgat
     1321 gaactcgcga ttctcctgcg tcagcttctt cttgtcccgg gcgacttcct gataggcccg
     1381 gctcaaccga agccgaggca tgccggtgtc gttcaggaac acgtggtagc ggtcggcgat
     1441 cttttcgacg atgagatcgg ggatgatgta tccgtcgctt cgctctgagt gcttcaggcc
     1501 gggcttgggg tcgaaccgag ccagctggtc cgccgcactc tgcaccccgc cgggttcgac
     1561 gccgaatcgc ttcgccaggt cgttccagcg gtgggcgatc agatccggga aggcctcggc
     1621 caccagacga taggtgaggg actcggtgtc gccggcctca cggagctgga tcaggaggca
     1681 ctcgcggaga tcccgggcgc ccacccccgg cggatccagc ttctgcacga tcgcgagcat
     1741 ctcctcggct tctgcgagcg tataaagcgc tgctccggac gccggcgacg ggagtgcggc
     1801 ctcatcgacc atctcgtcca gagcgtcttc cggctcgttc ggccgttcga aatgggtggc
     1861 gaccatctga ttgaccgatc cgaccagctc ctccagcgag gcggcgagat agccgtcttc
     1921 ggtcatgttg cccaggaatt cctcggccag tagccgctgg cgcggcgtga gctccatcaa
     1981 ttggagctgc tcgcggagat ggtcggtgag gttccgggtc tcgaccgtaa caggctcggt
     2041 gtactcctgg acctcgaact gctcccgggt gcccccgaca tcgaacccgt tgaggaggat
     2101 ttcctcccag tccatctcgt cgtccttctg ctgctgctct ttttcctttt ctttgtcctg
     2161 ctcctgctgc ttctcctgat cggcggactc ttcgtcttcc ggctcgagca gctcgaggaa
     2221 cggattggcc agcagctcct gcttcaggtg ctgctgaagg tccatcatgg gcatgtacaa
     2281 catgtccatc gcctgataga gccgcggatt gacccgcagc tcctggcgca ggccgatttg
     2341 ctgggaaaga ccggtgcgga ggctcacgcg gcgtgctcca aacgggcgcg cagccgcgtc
     2401 gtgagggtgg gtccgagata ccgttcagcc acgcgctcgt cgtagaccag ctcgcggacg
     2461 gtacccgcgg cctgaacctt cccttcgaac atgatgtacg cgcgatcaac gatgtccaga
     2521 gtctgctcca cgttgtgatc ggtgatcaac acgccgatgc cgcgatggcg cagcccggcc
     2581 actatggttt gaatatcgtg cactgcaatc ggatccacgc cggcgaatgg ctcatccagg
     2641 agcatgaacc tgggctcggt caccagcgcc ctggtgatct cgaggcgacg ccgctcgccg
     2701 ccgctcaagg cgtaggcttt gaccttccgc aagtgccgga tccccagctc gtccagcatg
     2761 cgctcgagcg atcgctgacg ctcggtccga ttcgactttc gagtctccag aattgccaga
     2821 atgttctcct cgaccgtcat gcgacgaaag accgacggct cctgtgccag gtacccgatc
     2881 ccgatgcggg cgcggcggaa catggccgct ctggtcaagt ccgtcccgtc gagaagcacc
     2941 cgtccggcct cggggcgaat caggccggtc aacaggtaga acgtggtcgt cttgccggcg
     3001 ccgttcggtc cgagcagtcc gacgatctct ccctgctcga gccggaggca gacctgatct
     3061 acgactctgc gaccgcgata actcttggtc aggtccaacc cctcgagcac gctgccgctg
     3121 ggagaagacg ccggcggttc cacggcaaga aggtcgcggg ctcggtgggc cacaccgagc
     3181 agcgcggcac ccaccatgtc gcgatccttc actgcgtccg accaccaagc cccggctgcg
     3241 cggacttcct cccactccgg acgagtgtct tcgatccatc cgtccagggc caaccgggga
     3301 agcacggagg tcgttacgtg cgcccgcacc gactcgccca gaccctcggc aggccgatat
     3361 accctgaggt acccctcgcg gtatgccagg agcagccggg tcaggctcga acgcccgtct
     3421 tcgtcggccg cggcaaccag cgaggccact gccagtggca gcgaggggtc ggccccgtcg
     3481 aactgcgcga gccatagcgg ctgattcatg gctgggcccg ggtggtgtcc ctggccgcgg
     3541 ccttttcttc agaaggctcg agctgcaccc cgtcggcttt gccgcgtatc tggaccaggt
     3601 ccaccgcttc cgatccgtcg gacttcatcg tgatcacgat ctcatcgccg cgagcgtaat
     3661 tgatgctcgg ccgctcgccg ggctttttct tctcattgga ctcgaggtgg tacgatcgtg
     3721 ccgagccccg agctaccaac ttggtcaaca ccgccttgct gcttccggcc gagtcgtgga
     3781 ccgcaaacgt ggcgatcaca gtatcgccgg tcagccagtc gcgttccttc gtcagggaat
     3841 cgacctggcc gcccagccat gccttgccga aggcgcgcgc ctccttgagc tgctggtccg
     3901 gcgaatcgag cgcaatcgag tcagccttga tctcgtactt ggccgacttc gcataaggcc
     3961 tgatggtgtc gccccacgcc tcggtatgga ccagctttcg atcgaccagg tcgtgcacga
     4021 tggtgtcggc gaccagggtc caatccttgt tcacggcctt gctatcgccc ttggcgacaa
     4081 cccggtcgag gtcccggttc ttgagccgaa agtcaagccg gcggccgctc aagtcgaagc
     4141 tgtcggaacc gagcccgtgg agctcagggc ggctcccgag cagcgtgccg tcgttggcag
     4201 ggccggtatc aagccgcaac gaatcgcccc ttgccgcaaa atcacttcga tccaccgtca
     4261 ccttaccacc gccccagatc cggtcgtctc ccttgatccg tactcgatcg gcgacgatca
     4321 cgtatggctc cgccggcttc ccggtcgaat cggttgccgc cgaatcacct cgggtcgagt
     4381 ccttcgcgta gtactcgatc cgagggcggc tcactgcgta catctcggtc gtgtctcgta
     4441 cacctttgac cgcccggtag taatcgagcg aagggccggt cagggtggag ccggtcttgg
     4501 tgtttcgggt cttcacgttg cggcgcgctt cccagcgttc gtccgaccga tagtaggtgc
     4561 cgaagtcggt gtccatcgcg agcgtggtgt cgcggtagcg cacgtggccg ttgaagacca
     4621 ctgtcttgcc cgcgtaggac gcaacgctgt cgctgaacat cgtgatcttg gtgcctcggc
     4681 aactcagccg gacattcccg cccgcgaagt agttgatacc cccactggtc tcgaccgcga
     4741 tgccttgccg gtccacgttg tcgatctgca gcgtgcaccg atccgaggtc tggccttcga
     4801 gcgggccggc cgcggctgct acgacgattg acactgccag ccatctcatt gctgacccgg
     4861 tagcagcaca cctttgccgg ctacgccgtg agggcgattg gtcacgacgt tcttgaaatc
     4921 cggatccgag ctgaagctgg tgccctggat gaactcgttg tcgtgcaaga accggaacgc
     4981 ttgatcggtc gagatctcgt tctttttcgg gtcgaacttc agcgtctcgg tctcgagctt
     5041 tcgcccgtct gtcgcaatga ccacgacttt tccgtccgcc tgcatcgagc cgtcctgcca
     5101 gcgatacgta ccatgatcgg cggtcagctt ggacgactca gcgcctttcg cgtcgtagaa
     5161 cgttgcccgc agcttccgca gcaaggcgag ctggctcgcc tcatagaagt acgccgtgtc
     5221 ggcctctata tgactcttgc gcacgccgtt ttcagtcaca tagtgcgaaa agccttccag
     5281 cacctgatcg gccgtgtccg cggcctgaac cgttgcagta ggccgggctc ccccgctctc
     5341 acacgccacg actgacccgc acgtcgccgc gacaaccagg atgactccca ggcgcaccgg
     5401 tccgcggctc gctctgcgca tcagcgtgcg acggtggcga gctccttggc ttcgacccct
     5461 gccccgcccc gccggcgctt gagggcagcg ccgatgaatc cgacgaacaa cgggtgcggc
     5521 cgggtcggcc gcgacttgag ctcagggtgg aactggcatc cgatgaacca gggatgatcc
     5581 ggcagctcga tcgtctccac caggccgcca tccggcgact gccccgaaag ccgcagtcca
     5641 tactctgcga gtacgtcgcg gtaggcgttg cttacctccc aacggtgccg gtgccgctca
     5701 ctgatttctc ccgctccata ggcctgcgcc actttggatc cgggccggag cttggccgtg
     5761 taggcgccga gccgcatgga gccgcccagg ttggagacgt cgcgctgcga gggcatcagc
     5821 gatatcaccg gggactcgca ctcgggcgcg aactcggtgc tgttggaatc gggcaggcgg
     5881 catacgtcgc gggcgaactc gatgatcgcc acctgaaggc cgaggcaaat cccgaagaac
     5941 ggaaggtcgt gctcgcgggc ccagcggatc gcctcgatca tgccttctac tccccgaatg
     6001 ccgaacccgc ccggaatcag cagcccgtcg aagttctcca gcaccctgcc cgcggtctcg
     6061 gtatcggtaa gctgatcgct cgaaatccaa tggatctcga caccggcgtc gtgggcaata
     6121 ccgccatgaa tcaaggcttc ctgcactgac ttgtaggcgt cgtgcaggtc ggtgtactta
     6181 cccaccaccg cgattcggac ccgatgggac ggattgagaa tccgctgggt gatctctttc
     6241 cacggcttca aatccggctc agccgtggtg aggcggagtc gctcgctcac gacgcggtcg
     6301 agcccctgct cgtggaagcg aagcgggatc tcgtagatcg agcgcacgtc cggactctcg
     6361 actacacagt cgaagtccac gttgcagaac agcgcaatct tccgcttgat gtccgccgag
     6421 atcggctgct cgctccggca gaccagaatg tccggcatga ttccgatctg catcagctcg
     6481 cggaccgagt gttgcgtcgg cttggtcttg agctcgccgg cggccgcgat gaacggcacc
     6541 agcgtaagat ggatgaacag ggcgttgtcg cgcccaacct cttggcggaa ctggcggatg
     6601 gcctcgagaa acggcagaga ctcgatgtcg ccgacagtgc ccccgatctc ggtgatcacc
     6661 acatcgtgcc ccggcgcagt gcgccggatt gcgttcttga tttcgtcggt gatgtggggg
     6721 atgacctgga cagtgg
//
LOCUS       DASRJQ010000641         3992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1803104, whole genome shotgun sequence.
ACCESSION   DASRJQ010000641 DASRJQ010000000
VERSION     DASRJQ010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1803104"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="VFB89_14520"
     CDS             <1..886
                     /locus_tag="VFB89_14520"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZM28559.1"
                     /translation="TLELSADVGQIRADAGQLEQVLLNLALNARDAMSHGGSFTVETY
                     RTELTPAYARLKPEAAVRPGWYAVLAVSDTGHGMDRATSAQIFEPFFTTKGTGQGTGL
                     GLSTVYGIVKQSEGYIWVYSEPGQGSTFRVYLPLAGESTGQAEPARTTAVPTDAVPAG
                     SGEPILVLEDDDAVRQIARRALEGAGYTVLAAADGPHALEMLGQATQPIRLALIDLVL
                     PGMSGLDVAGRMASTSPATRVIFTSGYPDGEIARRGLLAPDAAFIQKPFTPEALLRLV
                     RQELETTPATGDPATRSS"
     gene            complement(870..1529)
                     /locus_tag="VFB89_14525"
     CDS             complement(870..1529)
                     /locus_tag="VFB89_14525"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HZM28560.1"
                     /translation="MTYQLPRQAAALLLTLSLFAACKSNRTKMSEETGMRSTATPPVD
                     TGMVPANPNAAPADTARGAAGTTGTLSDANIMALLDEANKADSAAGAVAAKKATSPEV
                     KAFAKQMMIDHHKLREGGAKLAKKLNITPQLPANDPLAPAAQDESSTLQSTPKGAEFD
                     RVYIEKEVSVHQAVKDLLDQAHRATQNKDIQQLIETATPLISQHLDHAQRLQKQLTKT
                     V"
     gene            1902..>3992
                     /locus_tag="VFB89_14530"
     CDS             1902..>3992
                     /locus_tag="VFB89_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28561.1"
                     /translation="MVTFLKGLRRPENQAAAGVAGSRSDRPMSAREFAALEALVARAE
                     AAAAQLRSVSNITETGEALAAMHQRFAAVEGQLTGLEQLAARFSTAEEQAERAAKTQA
                     RTETQLAHADDSVQRIQSQLGGVSDKLDRALLLRDELEKFLGLEGPMSSIRSDADTLR
                     TQLMDLADGVARMRAQHDDALRAHRHTTSRLESFDQDHQAAASRLEEVARRVQGLERA
                     LEPLSHATDSIPTIQHQLAVLKALADQVAHKAATLEQQREAVDRAAGQISQLTRLDRE
                     LDVWLRRQEEQIRRFGTIEAKLAEVQAVQGKVIARNDELLEGQQQLDEGQRTARQALT
                     DLREQMRKSSEGFELENRGLHAVSERIADLRGAVKECEARFAVLDAASQGAAAVQAQV
                     RAATDEAAQLTQELVRLCDEARRIGTLRQDAERLEALAAEVTTGMRRIEETKPQVDDV
                     TQQLGALKGTSEMMADGLEQMRLAYEEMARLREAHAETQSWLSNADVWTRKVQAQVKE
                     LSGMEPGVERIRAEVEQVQAAMGDIEARRELLETVNGRLGELSTQNADLKARTDSLRT
                     RMDGAESRFNQLARQADEAQRVSDVIGEVAGSVGRVEHRIGSIDESVRALEGRTQQLD
                     ELEERIRLLGQEIEQRQGALDRATEHLSRASTLRQETAAVAQQLEELSRTIGSSLTEA
                     EGRSTQLERISGDLE"
BASE COUNT          689 a         1278 c         1359 g          666 t
ORIGIN      
        1 caccctcgag ctgagcgccg acgtaggcca aatccgcgcc gacgccgggc aactggagca
       61 ggtcctcctc aacctcgccc tcaatgcccg cgacgcaatg tcccatggtg gaagtttcac
      121 cgtggagacc tatcggaccg agctgacgcc tgcatatgca cgactcaaac ccgaggccgc
      181 ggttcggccg ggctggtatg ccgtgctggc agtgagcgat accggacatg gaatggaccg
      241 cgccacgtcc gcgcagatct ttgagccctt tttcacgacg aagggaaccg gacaagggac
      301 cggacttggc ctatccactg tctacggcat cgtgaagcag tcggaagggt acatctgggt
      361 gtacagcgag ccgggtcagg ggtccacgtt cagagtgtat ttgccgctcg ccggcgagag
      421 taccgggcag gcggagccgg ccagaaccac ggcggtaccg actgacgccg taccagcagg
      481 atccggtgag cctatcctcg tactcgaaga cgatgacgcc gttcggcaga tcgctcggcg
      541 ggccctggaa ggagccggct acacggtatt ggctgcggcc gacgggcctc acgccctcga
      601 aatgctgggc caggctacgc agcccattcg gctcgcgctc attgatctgg ttttgccagg
      661 tatgagcggg ctcgacgtcg cgggacggat ggccagcacg tcgcccgcga cccgggtgat
      721 cttcacctcc ggttatcccg atggtgagat cgcccggcgc gggctgcttg cacctgacgc
      781 cgcattcatt cagaaaccgt tcacccccga ggcgctcctc aggctcgtgc ggcaggagct
      841 ggaaacaact cctgccacgg gcgacccagc tacacggtct tcgtaagctg cttctgcagg
      901 cgctgggcgt gatcgaggtg ctggctaatg agcggtgtgg ccgtctcgat caactgctga
      961 atgtccttgt tctgggtggc ccggtgcgcc tggtccagca ggtccttcac cgcctggtgc
     1021 acgctgacct ctttctctat gtagacccga tcgaactcgg cgcctttggg ggtcgactgc
     1081 agcgtgctcg actcgtcctg ggcggcaggg gccagcggat cgttggccgg cagctgcggc
     1141 gtgatgttga gcttcttggc cagcttggcg ccaccttccc gcagcttgtg gtgatcgatc
     1201 atcatctgct tcgcgaatgc tttcacctcg gggctggtgg cctttttcgc ggcgacggct
     1261 ccagccgcgc tgtcggcctt attggcttcg tcgagcagcg ccatgatgtt ggcgtccgaa
     1321 agcgtgcctg tagtgccggc agcgccccgg gcggtatccg ccggcgccgc gtttggattc
     1381 gcgggcacca tgccggtgtc aacgggcggg gttgcggtgg accgcatacc cgtttcttca
     1441 ctcatcttgg ttcgattgct cttgcacgca gcgaacaggg acagcgtcag gagtagagct
     1501 gcagcctgtc gcgggagttg gtacgtcata ctctcctcca gatgcctgag ttcaggcaaa
     1561 caaatcgtac gaacgcctga catctcagat tagaggtcgg gatcggggcc ggtggtgacg
     1621 cctctcaccg gcttatcggg cgcactcgat tgccgcgacc tgccgtaacc ttggcacgtc
     1681 gccagcggcg ctccccataa tcgcttccgg gcccgggccc acttcggctg gccaatccgt
     1741 ctcatcttcc gcgtctgcag tgagttggcg cgcgtcgttc cgccgttccc cgtcacccca
     1801 gccggttcgc cgccgggcat acccgttgca gacaggtccc cagtccagcc cgattccaat
     1861 cttcaccacg cgtacgcgcg cttcccgtcg gagtcaacgc catggtcacg ttcctgaaag
     1921 gtctgcgccg cccggaaaat caggcggcgg ccggtgtcgc cgggtcccgc tccgatcggc
     1981 caatgagtgc ccgcgagttc gccgcgctcg aggccctggt cgcgcgggcg gaggctgccg
     2041 cggcgcagct tcgctcggtc tcgaacatca ccgaaaccgg cgaggcgctg gccgccatgc
     2101 atcagcggtt tgcggcggtc gagggacagc tgaccgggtt ggaacagctt gcggccaggt
     2161 tctcgaccgc cgaagagcag gctgagcggg cggcgaagac ccaggcccgt accgaaaccc
     2221 agctggcgca cgcagacgac agcgttcagc ggattcagtc gcagctcggc ggggtcagtg
     2281 acaagctgga tcgggcgctg ctgctccggg acgagctgga aaagttcctc ggactcgagg
     2341 gcccgatgag ctccatccgc agcgatgcgg atacgctccg aacgcaactg atggatcttg
     2401 ccgacggcgt ggcccggatg cgcgctcagc acgacgacgc cctccgagcc catcggcaca
     2461 ccacctcccg actcgaatcg ttcgatcagg atcaccaggc cgcggcaagt aggctggaag
     2521 aagttgcccg ccgcgtccag ggactcgaga gggcactgga gccgctcagc cacgcgaccg
     2581 actccattcc cacgattcag caccagctcg ccgtgctcaa ggcgctggcc gaccaggtcg
     2641 cgcacaaggc cgcgacgctg gagcagcagc gtgaggcggt cgaccgggcc gccggtcaga
     2701 tctctcagct cacccgtctc gatcgcgagc tggatgtctg gctccgtcgc caggaagagc
     2761 agatccgtcg gttcggtacg atcgaagcga agctggcgga agttcaggcg gtgcagggca
     2821 aggtgatcgc ccgtaacgac gagctgctgg aaggacagca acagctcgac gagggccaac
     2881 ggactgcacg ccaggcgctg accgacctgc gggagcagat gcggaagagc agcgaagggt
     2941 tcgagctgga gaatcgcgga ctccacgcgg tgagcgagcg aatcgctgat ctccgcggcg
     3001 cagtcaagga gtgcgaggcc cggtttgccg tcctcgatgc ggcgagtcag ggtgctgcag
     3061 cggtgcaggc ccaggtccgg gccgctaccg atgaggcggc gcagctcacc caggagctgg
     3121 tgcggctgtg cgacgaagcg cggcggatcg ggacgctgcg gcaggacgcc gagcggctcg
     3181 aggcgctggc agccgaggtc acgaccggga tgcggcggat cgaggagacc aagccccagg
     3241 tagacgacgt cacccagcag ttgggtgcgc tcaagggtac cagcgagatg atggcggatg
     3301 gcctggagca gatgcggctg gcctatgaag aaatggcccg gctgcgggag gctcacgccg
     3361 agacccagtc ctggttgtcc aatgccgatg tctggactcg caaagtgcag gcgcaggtca
     3421 aggagctgag cggaatggag cccggcgtgg agcgaattcg cgccgaggtg gagcaggtcc
     3481 aggccgccat gggcgacatc gaggcccgcc gcgagctgct ggagacggtg aacggtcggt
     3541 tgggtgagct ctcgactcag aacgccgatc tcaaggctcg caccgacagc ctgcgcaccc
     3601 ggatggacgg cgccgagtcg cggtttaatc agctggctcg tcaggccgac gaagcgcagc
     3661 gggtttcgga cgtcatcggc gaagtcgccg gatcagtggg gcgcgtcgaa caccggatcg
     3721 gttcgatcga cgagtcggtc cgtgcactcg agggacgtac gcagcagctc gatgagctgg
     3781 aggagcggat tcgcctcctg ggccaggaga tcgagcagcg gcaaggtgcc ctcgatcgtg
     3841 ccaccgagca cctctctcgg gcctcgaccc ttcggcagga gacggcggcg gtcgcccagc
     3901 agctcgagga gctgtcccgg accatcggca gctccctcac cgaagccgag ggccgctcga
     3961 cccagttgga gcgcatctcc ggcgatctgg ag
//
LOCUS       DASRJQ010000642         2949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2078232, whole genome shotgun sequence.
ACCESSION   DASRJQ010000642 DASRJQ010000000
VERSION     DASRJQ010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2078232"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..294
                     /locus_tag="VFB89_14535"
     CDS             109..294
                     /locus_tag="VFB89_14535"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM28562.1"
                     /translation="MRDMNLDLKLTLHHARHAWAVRKVRARVPVAVVQAQLGHSSPML
                     TLRRYGAFIPDGADRAH"
     gene            complement(430..502)
                     /locus_tag="VFB89_14540"
     tRNA            complement(430..502)
                     /locus_tag="VFB89_14540"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(467..469),aa:Lys,seq:ttt)
     gene            544..948
                     /locus_tag="VFB89_14545"
     CDS             544..948
                     /locus_tag="VFB89_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28563.1"
                     /translation="MQLKFMRFGLLLCSLALVAARATAQTNKNADRWQVTYDTGQILW
                     DVRLVKLAGDSLQVRRGDSLAVVPVGKITELRLIKKSEMELGAPGGGAMAALMGSDDE
                     VYDLTPLEFSDRLRVVQKIFLYHPPDSAAAQP"
     gene            952..1614
                     /gene="bioD"
                     /locus_tag="VFB89_14550"
     CDS             952..1614
                     /gene="bioD"
                     /locus_tag="VFB89_14550"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HZM28564.1"
                     /translation="MNETVLVAGTDAGVGTTWVACALARTLRQAHRSVIAVKAVETGC
                     GDQPFETEDGVRLAAATGQREPTRALRRFRAPTIPAAAAELEGATIDLDELAGEIEAL
                     AKDGVLTLLEGSGGLLAPFGWDWNVVDLAQVFEATALVVASDRLGAVNHTLLNLSALD
                     LAGLRVAGIVLNAPPESDATTGSNASAIARISGIDRVREVPRLDKPENASPWFQEVAG
                     WL"
     gene            1605..2366
                     /locus_tag="VFB89_14555"
     CDS             1605..2366
                     /locus_tag="VFB89_14555"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZM28565.1"
                     /translation="MALNRFAIAVLSLAGPLSAQAPKTITFASTDSLPVTADLYAPLP
                     PTAPFIVLFHQAGASRGEYREIAPKLVAMGFNCMAIDQRSGESSKGVRNETAKAADER
                     HLLVSMLSARQDLESALRYARGHYARGPVLAWGSSYSAALVLVVAGQDRGIADGVLAF
                     SPGEYFPVAPGNPTLVTDAARAIKVPVFITSARSETADWRPIYAALNSSLRRAYVPET
                     DGHHGSSALWKETPGQEGYWQAVRGFLNRQFPRSK"
     gene            complement(2383..>2949)
                     /locus_tag="VFB89_14560"
     CDS             complement(2383..>2949)
                     /locus_tag="VFB89_14560"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZM28566.1"
                     /translation="VTGLGYATVSDSGAAMRPIVERLSILDQVWLARISRQAAPAWMV
                     RWLRVATLAGGATVTTSVPLLLLPWSGSRSFGLALLLANATSHACVQLLKRTVVRRRP
                     HLSCDGVLALGVIPDAFSFPSGHSAAAMALAVPCLVRGGAAGVPALLLALVVGASRVC
                     LRVHYPSDVVAGQILGVAGGVFAVWIVY"
BASE COUNT          535 a          955 c          941 g          518 t
ORIGIN      
        1 ttcttgttca gagcggcgtc agcctggtgc gggtgtcgtc gaacacgata gatccgtctg
       61 ccgcggcact attgactttc ctggtggtct ccagggcaca atcgggcggt gagagacatg
      121 aaccttgacc ttaagctcac cctgcatcac gctcgccacg cctgggccgt gcggaaagtc
      181 cgggcccgcg tcccagtcgc cgtcgtccag gcgcagctcg gtcactcgag cccgatgctc
      241 acgctcaggc ggtatggcgc atttatcccg gacggcgcgg accgggcgca ttaggtcgcg
      301 gttgtcgaac gctaccagac ccgcagaatc ggggtgctat agtgcagcga tcgtgtaatc
      361 gagaaggggc ccgccctcac cgacccagcc cctttcgcgt atgtggtggc taccatatag
      421 cttaccgcat gagggcggtg ggaatcgaac ccacgaccta gggattaaaa gtcccttgct
      481 ctaccaattg agctacgccc tcgcccccca aacgtgatcg gccccttccg gaaactcaag
      541 acgatgcagc tcaagttcat gcggttcggc ttactgcttt gctctctggc gctggttgct
      601 gcccgggcca ctgcacagac caacaagaac gctgaccgct ggcaggtcac atacgacacc
      661 ggtcagattc tttgggacgt tcgtctcgtc aagctggcgg gtgattcgct ccaggttcgg
      721 cggggcgact cgctcgccgt cgtcccggtc gggaagatca ccgagctgcg cctcatcaag
      781 aagtccgaga tggaactggg tgcgcccggc ggtggcgcca tggccgcgct gatgggaagc
      841 gacgatgaag tgtatgacct gacccccctg gagttctccg accgactgcg ggtggtgcag
      901 aagatctttc tctaccaccc gcccgactcg gcggcggcac agccatagga cgtgaacgaa
      961 accgttctcg tggccggcac cgatgccggc gtcggcacaa catgggttgc gtgcgcgctc
     1021 gcgcgaaccc tgcggcaggc ccaccgatcc gtaatcgcag tgaaggcggt ggaaacgggc
     1081 tgcggtgacc aaccttttga gacggaagac ggcgtccgtc tggctgccgc taccggacag
     1141 cgcgagccga cgcgggcgtt gcggcgcttc agagccccga cgataccggc ggcagccgct
     1201 gagctcgagg gcgcgacgat cgatctcgac gagctggcag gcgaaatcga ggcgctcgca
     1261 aaggacggcg ttctcacttt gctggagggc tcgggtggac tcctcgcccc attcggctgg
     1321 gattggaacg tggtcgatct ggcccaggtc ttcgaggcga cggcactggt tgtcgcaagc
     1381 gaccgactcg gcgctgtcaa tcatactctc ttgaatctga gtgccctcga tctggcaggc
     1441 ttgcgggttg ccggcatcgt gctcaacgct ccgcccgaat ccgatgcgac aacgggttcc
     1501 aatgcctccg ccatcgcgcg gatttccggc atcgatcggg tgcgggaagt gcctcgactg
     1561 gacaagcccg agaacgcgag tccctggttt caggaggttg cgggatggct ctgaatcggt
     1621 tcgctattgc cgtactatcc ctcgccggcc cgctttccgc tcaggcaccg aagacgatca
     1681 cattcgcgtc gaccgacagc ctgcccgtta cggctgacct ctacgcgccc ctgccgccga
     1741 cggctccgtt catcgtcctg tttcatcagg ccggcgcaag ccgcggagag taccgcgaaa
     1801 tcgcccccaa gctggtggcc atgggtttca actgcatggc catcgatcag cgctcggggg
     1861 aatcttcgaa gggtgttcgc aacgagactg ccaaggcggc ggacgagcgg catctgctgg
     1921 tgtcgatgct ctccgcgcga caggatctcg agagcgcctt acgctacgcg cgcggccact
     1981 atgcgcgcgg ccccgtgctc gcctggggca gctcgtattc ggctgcactc gtattggtag
     2041 ttgccgggca ggatcgcggg attgcggacg gcgtgctcgc gttttcaccg ggcgagtact
     2101 tcccggtagc cccgggcaat ccaacgttgg ttaccgacgc ggccagagct atcaaagttc
     2161 cggtcttcat cacgtcggcc cgctccgaga ccgccgactg gcggccgatt tatgcggcgc
     2221 tgaactcctc acttcgtcga gcgtacgttc cggaaaccga tgggcaccat ggctcgagcg
     2281 cactctggaa ggagacgccg ggtcaggaag gctactggca ggcagtgcgt ggattcctca
     2341 accgtcagtt ccctcgcagc aagtaagcgt tcggcacccg cctcagtaga caatccacac
     2401 tgcgaacacc ccgcccgcca cgccgaggat ttgtccggct acgacgtcac tggggtagtg
     2461 tacccgcagg cagacccgtg acgctccaac caccaatgca agcagcagtg cggggacccc
     2521 tgcagcgccc ccgcgaacaa gacatgggac ggcgagcgcc attgctgccg ccgaatggcc
     2581 ggaaggaaac gagaaggcat ccgggatgac gccaagcgcg agcacgccgt cacacgaaag
     2641 atgcgggcgc cgccgcacga ccgtgcgctt gaggagttga acgcaggcat gagaggtggc
     2701 gttcgcgagg agtagggcga gaccgaagct gcgcgagccg ctccagggga gcaggagcaa
     2761 gggtaccgat gtcgtgacag tcgcgccgcc tgccagtgtg gcaacgcgaa gccatcgtac
     2821 catccaggcc ggcgcagcct gcctcgagat gcgcgccagc cagacctggt ccagtatgct
     2881 gagccgctcc acgatcggcc gcatcgctgc cccggaatcc gacaccgtag cgtatccgag
     2941 cccggtcac
//
LOCUS       DASRJQ010000643         2494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1884493, whole genome shotgun sequence.
ACCESSION   DASRJQ010000643 DASRJQ010000000
VERSION     DASRJQ010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1884493"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VFB89_14565"
     CDS             <1..190
                     /locus_tag="VFB89_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28567.1"
                     /translation="TVLLDGTGKKYTPIGWDGAAPGGHHREGVLRFKPVSSKPRSIEI
                     QITRAGEAGPRSFRWELN"
     gene            223..564
                     /locus_tag="VFB89_14570"
     CDS             223..564
                     /locus_tag="VFB89_14570"
                     /inference="COORDINATES: protein motif:HMM:NF023095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2933 domain-containing protein"
                     /protein_id="HZM28568.1"
                     /translation="MTKLFASSAIAICGKEKLMNHGHHNEPGGADKPPSRFKWVFIGF
                     AAIAAYLLITEHQTHVSGWLAKYGIWLVLLACPLMHLFMHGGHGGRGGHRSQRDDEAQ
                     ASDKQPKGQAK"
     gene            561..1226
                     /locus_tag="VFB89_14575"
     CDS             561..1226
                     /locus_tag="VFB89_14575"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HZM28569.1"
                     /translation="MNPSESSAYGLWSLVIINSLVFITFAFSFAKPQSPRDWRSFGAF
                     SAFLVALFTEMYGFPLTIYLLSGWLSTRFPGVDFLAHDSGHLLEVMFGSRDNPHFGPF
                     HLLSSVFIFGGFIVLASAWNVLYQAQRRHRLADTGLYARVRHPQYVGFILIMLGFLLQ
                     WPTLVTLVMFPILVTMYVLLARREEREALAAFGDEYRRYMARTPSFLPRVGELFTSTT
                     EKR"
     gene            1309..1566
                     /locus_tag="VFB89_14580"
     CDS             1309..1566
                     /locus_tag="VFB89_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28570.1"
                     /translation="MNDLWKVHAFSLATTLGAAYVLCAIFDVLFPPFGLLAALASASP
                     WPISGSPLAYLTGFVLVTAAGFALGALHGIAWGFWSKRLHG"
     gene            1632..>2494
                     /locus_tag="VFB89_14585"
     CDS             1632..>2494
                     /locus_tag="VFB89_14585"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HZM28571.1"
                     /translation="MSAQGILIAGGYGVVGGRIAAELAPDYPDQVIVAGRNLDLAKAT
                     ATVIGHGACGRALDVTVPSSIAAALGNVAVVVSCIDQPGRRLLHAAVERGLRYTDISP
                     HLTELGRGAAYEKIDDAARASGARVVLGTGIVPGIANVMVRALAETLGGAEEIETSLL
                     LSASDTAGAASFDYFLQELSMPFEVHVDGADHPARAFSDPRLVEFPPPVGERPAYLFP
                     FSDQVLYPRTMGVRTALTRLAIEPTRLAKLLAFLTRTGAADLIAREPVRHAIAKQRKH
                     LATRADTQFALR"
BASE COUNT          437 a          794 c          758 g          505 t
ORIGIN      
        1 cacggtcctg ctggatggaa ccggcaagaa atacaccccg atcggctggg acggtgcggc
       61 cccgggcgga catcaccgag agggcgtgct gcgtttcaag cccgtctcct cgaaaccgcg
      121 gtcgatcgag atccagatca cgcgcgccgg ggaagcgggg ccgagatcgt ttcgctggga
      181 actcaactag acgcgaccgt ctgtaacctc gcggaggcat tcatgaccaa gctgttcgca
      241 tcctcggcaa tagcgatttg cggaaaggaa aaactcatga accatggtca ccacaacgaa
      301 ccggggggcg ccgataaacc gccgtccaga ttcaaatggg tatttatcgg atttgccgcc
      361 atcgcagctt acttgctgat taccgagcac cagacgcacg tctccggatg gctggcgaag
      421 tatgggatat ggctggtcct gctcgcctgt ccgctcatgc atttgttcat gcatggagga
      481 catggcgggc gtggcgggca ccggagccaa cgcgatgacg aagcccaggc gtcggacaag
      541 caacctaagg ggcaagcgaa atgaatccct ccgaatcctc tgcctacggg ctgtggtcgc
      601 tcgtcatcat caactcgctg gtgttcatta ccttcgcgtt cagcttcgcc aagccgcaga
      661 gcccgaggga ctggcgctcc ttcggcgcat tttctgcgtt cctcgtggcg ctgttcaccg
      721 agatgtacgg cttccctctg acgatctacc tgctgtccgg ctggctcagc accaggtttc
      781 ccggcgtcga cttcctggcg cacgattccg gacacctgct cgaagtcatg ttcggctcgc
      841 gcgacaaccc gcatttcggg ccatttcatt tgctgagttc cgtcttcatc ttcggcggct
      901 tcatagtgct cgcctctgcg tggaacgtgc tctaccaggc acagcgcagg caccgtttgg
      961 ccgatacggg tctgtacgcc cgcgtgcggc acccccagta cgtcggcttc atcctgatca
     1021 tgctgggatt cctgctgcag tggcctaccc tcgtcacgct cgtcatgttc cctatcctcg
     1081 tcacgatgta cgtcctgctc gcccggcggg aggagcggga agcgctggct gcatttggcg
     1141 acgaataccg gcggtacatg gcgagaaccc cgtctttcct gccacgcgtg ggtgagctgt
     1201 tcacgtccac cactgaaaag cgatagagga tggcggtggg gcgctgtcgg ccggtgagat
     1261 accttgacgt caccgccctg aaatcccatg aaaaggagca tgaccaccat gaacgacctc
     1321 tggaaagtgc atgctttttc gctggcgacg actctgggtg cggcgtatgt cctgtgcgcc
     1381 attttcgatg ttctgtttcc acctttcggg ctcctcgccg cccttgcctc ggccagcccg
     1441 tggccgatct ccggcagtcc cctcgcttac ctgacgggat tcgtactggt caccgccgcc
     1501 ggttttgccc tcggcgcgct ccatggaatc gcctggggat tctggagcaa gagattgcac
     1561 ggctagacat tcgagacgct cggccgagcg ctggaggatc gacggaacat ggcacgcaca
     1621 ttgggaagga catgagcgct caaggaattc tgatcgcggg cggctacggc gttgtcggag
     1681 gccgtattgc ggccgagctg gcgcccgatt accctgacca ggtcatcgtc gcgggccgca
     1741 acctcgacct agcgaaggcg accgctacgg tgatcgggca cggtgcttgc ggccgcgcgc
     1801 ttgacgtgac cgttccgtcg tcgatcgctg cagcgctggg caatgtcgcg gttgtcgtga
     1861 gctgcatcga tcagcccgga cgtcgtctcc tgcacgcagc ggtcgagcgc ggactgcggt
     1921 acacggacat ctcgcctcac cttaccgagc tcggacgcgg cgctgcgtac gagaaaatcg
     1981 acgatgccgc tcgggcttcc ggagcacgcg tagtgttggg aacaggcatc gtccctggca
     2041 tcgccaacgt aatggtgcgc gcgcttgccg agacactcgg cggagctgag gagatcgaga
     2101 cgtccctgtt gctctcggcc agtgacactg ccggcgccgc ttcgttcgac tactttctcc
     2161 aggagctatc gatgcccttc gaggtccacg tggatggtgc ggaccacccg gcgcgcgcct
     2221 tcagcgaccc tcggctcgta gagtttccgc cgccggttgg agagcggccc gcatacttgt
     2281 ttccattctc cgaccaggta ctctatccgc ggaccatggg cgtgcgaacg gcgctcacga
     2341 ggctcgccat cgagcctaca agactggcaa agctgcttgc gtttttgact cgcaccgggg
     2401 cagccgacct catcgctcgc gaaccggtgc gtcacgcaat tgcgaagcaa cgcaagcacc
     2461 tcgcgacacg cgcagacacc cagtttgcgc tgcg
//
LOCUS       DASRJQ010000644         2191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1804425, whole genome shotgun sequence.
ACCESSION   DASRJQ010000644 DASRJQ010000000
VERSION     DASRJQ010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1804425"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..305
                     /locus_tag="VFB89_14590"
     CDS             129..305
                     /locus_tag="VFB89_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28572.1"
                     /translation="MTGRLMTLLAIAKLQCQQRDRGGAAATAARATSVLDRGNSDHAG
                     WIKKFEDELARCGP"
     gene            complement(332..2056)
                     /locus_tag="VFB89_14595"
     CDS             complement(332..2056)
                     /locus_tag="VFB89_14595"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM28573.1"
                     /translation="MMLNPRQAQGAIAGVMLLVMCAPAAVSEEILRSRLVAPAEPLTY
                     FDHTREGRRLFDEQKYSEAAEVFQKAVSQYPKDGSVWIQLGQALRLSNKPREAITAYE
                     KGLELTSTWQPAGVRLSLARAHLVLGNKEMTYRVLERMVQEDQYTQKPALYDHPAFVA
                     IREEPRFLNLVGRIDTSKMSRAEGWRTDIDYLVSEIKRVNHLYRKQPLPEELTARQRA
                     LKRDVGKLTDEEIFVGMGRMLSPLKQGHVSLAIFPETTRLAVRTIPLQFYAFPEGIFV
                     VRSDEKNADLVGAEVLRIEDAPPGELVKQIEEHASVENEMKMLWAGMRPLGSIPVLRG
                     LGVLKPGRDEVRLTLKKDGQTVERVLAPVAQEQERKLGPPPVATPPLFVRNVPRVHWL
                     ESLPKSDAVFVQVNQIAPDPDESMAEFGLKLRKFLADTPVKNVILDLRHNNGGNTFTY
                     TEVLRTLVAHSTKEGNRLYVIIGRGVYSATSNLISDLERLARPIFVGEPSSGIGNQDG
                     DESMTVLPYSGIRAFLTSVKWQYSHPWDLRTSMVPDIPVQLTAKDYFAGKDSVMETIL
                     ADIRRARP"
BASE COUNT          366 a          752 c          676 g          397 t
ORIGIN      
        1 gcgccgggga gcggccggag gccaccggac tcggcacctg cgaccggatt caggatctga
       61 gcggagcatg tcccggcccc tgattggact cgggaagctg tccatgatcg ccagccggcg
      121 cccccacggt gaccggacga ctgatgacgc ttctcgcgat cgccaagctg caatgccaac
      181 agcgagatcg gggcggtgct gcggccaccg ccgcccgcgc gacgagcgtg ctggaccggg
      241 ggaattccga ccacgccggc tggatcaaga agttcgagga cgagctcgca cgctgcggtc
      301 cgtaggccgc ccatcaggag cggcgagacg ttcaaggccg ggcgcgacgg atgtccgcca
      361 ggatcgtctc catcaccgag tccttcccag cgaagtagtc cttcgccgta agctggaccg
      421 gtatgtctgg aaccatggag gtgcgcaggt cccacgggtg gctgtactgc catttcaccg
      481 acgtgaggaa ggcgcggatg ccgctgtagg gcagcacggt catcgactcg tcgccgtcct
      541 gattgccgat cccgctgctt ggctccccca cgaagatcgg gcgcgcgagc ctctccagat
      601 cgctgatgag gttgctggtc gccgagtaga ccccccgacc gatgatcacg tagaggcggt
      661 tcccctcctt cgtgctgtgc gccacgagag tccgtagcac ctccgtgtag gtgaaggtgt
      721 ttccgccgtt gttgtgccgc aagtccagga tgacgttctt gacgggcgta tccgccagga
      781 acttgcggag cttcaggccg aactcagcca tgctctcgtc cgggtccggc gcgatctggt
      841 tcacctgcac gaacaccgca tcgctcttcg gcagggactc cagccagtgg actcgcggca
      901 cattccgcac gaatagcggc ggcgtggcca ccggcggagg accgagcttg cgctcctgct
      961 cctgcgcaac gggcgccaag acccgctcca ccgtttgacc gtccttcttg agagtgagcc
     1021 gcacttcatc gcgcccaggc ttcaacacgc caagcccgcg cagcaccggg atcgacccca
     1081 gcggccgcat cccggcccag agcatcttca tctcgttctc gaccgaagcg tgctcctcga
     1141 tctgcttcac cagctcgccg ggaggtgcgt cctcgatcct gagcacctcc gccccgacca
     1201 gatcggcgtt cttctcgtcg cttcgcacga cgaaaatgcc ttccggaaag gcatagaact
     1261 gtagcggaat cgtcctgacc gccagccgcg tggtctccgg aaagatcgcc agcgagacgt
     1321 ggccttgctt gagcggggac aacatgcgac ccatccccac gaagatctcc tcgtccgtca
     1381 acttgccgac gtcccgcttg agggcgcgct gccgcgccgt gagctcttcc ggcagcggct
     1441 gcttccggta gagatggttg actcgcttga tctccgacac gagatagtcg atgtcggttc
     1501 gccagccctc cgcgcgcgac atcttcgacg tgtcgatccg gcccaccagg ttcaggaatc
     1561 ttggttcctc ccgaatcgcc acgaacgccg ggtggtcgta aagcgccggc ttctgcgtgt
     1621 actggtcctc ctgcaccatg cgctccagca cccgataagt catctccttg ttgccgagta
     1681 ccaggtgcgc gcgcgccagg gaaagtcgga cgccggcggg ctgccaggtg ctggtcagct
     1741 ccagtccctt ctcgtaggcg gtgatcgcct cccttggttt gttggacaat ctcagggcct
     1801 gccccagctg gatccagacc gaaccgtcct tggggtattg ggaaacggcc ttctggaaga
     1861 cctccgccgc ctccgagtat ttctgctcgt cgaacagacg cctaccctcc cgggtgtggt
     1921 cgaaatacgt gagcggctcc gccggcgcca caagtcgcga gcgcaggatc tcctccgaca
     1981 ctgcggccgg ggcacacatg acgaggagca tgacgccggc aatcgcgccc tgcgcttgtc
     2041 gcggattgag catcatcgac cggctcagtc gttctcgtcc gcgaaggcct gcacgtgcac
     2101 cggcacgtcg tcgaccagca acagggcgcc tacgaggcgg tcgccctcaa gcctcacgtc
     2161 tgtgccgatg ccttccgacg ggaagctgtc g
//
LOCUS       DASRJQ010000645         2163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3494130, whole genome shotgun sequence.
ACCESSION   DASRJQ010000645 DASRJQ010000000
VERSION     DASRJQ010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3494130"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..2076)
                     /locus_tag="VFB89_14600"
     CDS             complement(151..2076)
                     /locus_tag="VFB89_14600"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 and transglutaminase domain-containing
                     protein"
                     /protein_id="HZM28574.1"
                     /translation="MRAVCVSVLLGVVLTALPSASRAWTPWNEISETDKSLTEVSFAP
                     GAGAVILEEEIQIALNETLEKSTVDVYRRIKILNESGMKEGTVSLHSSKHLRIKDLQA
                     RTILPDGLELALPKDAVLPAAASSSADQELVSFAMPGVTPGAIVEYRYREYIDGLPAR
                     RHRLQGSLPILTSRFSYELPRGQPLNYRYLGPPQFLADRRTRVGATTNTLIHEFKSLP
                     PVPDEEYSAPLIDRSASLIFDPSGMSGTARKKPEWEQIVLWAQSEGLKNYADVRRHDR
                     DASRLALDLAAGAKSAADKARVIYQFVRDRIGVSGSSGIWAGRNTADAILKSELGTAI
                     ERALLLQAMLRAAGIEAELGWSAHRTDGEILRQVVYPGVFDVFLVVAQIDGQAVYLAP
                     SEAKLPFGVLPATLEGAACLLVDQKPPVWVDLPERPASFTTRLAELTFAVNPKGGLTG
                     SGVLRITGHHAIAWFLNANAAKEDRFEHWRVWLAGRLAPVVVSIDEVREDSEAPSVEV
                     KWRAEVPDSDGAFSELAIPLAAPFAEQHNPFTLPASQRLSPVVLPFRESSEVTVRVSW
                     PPGWAIRPPLSHTLRTRAGSLSAAIQVDENERVLTSTRRLVFERTHFSVREYDQVKAL
                     YAAAVANDNELALVMHD"
BASE COUNT          376 a          697 c          704 g          386 t
ORIGIN      
        1 gcgatgctgc gcgtcgaggg ggacgcgatc gaggtgcgcg gcgccagcgg cgcccgcatc
       61 ttccagcgcg gcaccgagcc gatcgagatc aaacccgggt cgctgctgcc gccgctcggg
      121 ggtggctgat cgaccgcttt ccgccaggca tcagtcgtgc atcacgaggg cgagttcgtt
      181 atcgttcgcc accgccgccg cgtacaatgc cttgacctga tcgtactctc gcacgctgaa
      241 atgggtgcgc tcgaacacca gcctccgagt gctggtgagg acccgctcgt tctcgtccac
      301 ctggattgcc gcagataggg atcccgcacg cgtccggagc gtgtgcgaca ggggcggccg
      361 aatcgcccag cccggtggcc agctcacgcg aaccgtgacc tcgctggact ccctgaaggg
      421 aaggacgacg gggctgagtc gctgcgaagc cgggagcgtg aaggggttgt gctgctcggc
      481 gaacggcgct gccagcggta tggccaattc actgaaggcg ccgtccgagt ctgggacctc
      541 ggcacgccac ttgacctcga cgctaggtgc ctcagagtcc tcacgtacct cgtcgatgct
      601 gacgaccacc ggcgcaagtc gcccggcgag ccagaccctc caatgctcga accgatcctc
      661 cttcgctgca ttcgcgttga ggaaccaggc tatcgcgtgg tggccggtta tcctgagcac
      721 cccgctgccc gtgagtccac ccttgggatt cacggcaaag gtcaattcag cgagccgggt
      781 ggtgaaggag gctggtcgct ccggcagatc gacccacacg ggcggcttct ggtccaccag
      841 caggcaggcc gcaccttcca gcgtcgcggg cagcacaccg aacgggagtt tcgcttcgga
      901 aggggcaagg tagacggcct ggccgtcaat ctgggcaacc acaagaaaca cgtcgaacac
      961 gcccggatag accacctgac gcaggatctc gccatcggtt cggtgtgcgc tccaccccag
     1021 ttcagcctcg atcccggcgg cccgcagcat ggcttggagc agcagggctc tctcgatggc
     1081 tgtgcctagt tccgacttga ggatcgcgtc tgccgtattt cgtcctgccc agatgccgga
     1141 cgagcccgac acaccgattc gatcgcgcac gaactggtag atgaccctgg ccttgtcggc
     1201 ggcactcttc gcgccggcgg cgagatcgag ggccaggcga ctggcatctc ggtcatggcg
     1261 tcgcacgtct gcgtagttct tgagcccctc cgattgcgcc cacaggacga tctgttccca
     1321 ttccggcttc ttccgtgcgg tgccgctcat tccggaaggg tcgaagatca ggcttgccga
     1381 acggtcgatg aggggcgcgc tgtactcttc gtcgggcacc gggggcagcg acttgaattc
     1441 atgaatcagc gtgttggtcg tcgctccgac gcgtgtccga cgatcggcga ggaactgggg
     1501 tgggccaagg tagcggtagt tgagtggctg ccccctgggg agttcgtagc tgaagcggct
     1561 ggtgagaatg ggcaggcttc cctggagtcg gtgtcgcctc gccgggagac catcgatgta
     1621 ttcccggtat cggtactcga caatcgcccc cggagtgacc cccggcatcg cgaaggaaac
     1681 aagttcctgg tcggcagacg acgaagccgc cgccgggagg accgcgtcct tgggcaaggc
     1741 caactccagt ccgtccggca ggatagtgcg ggcctgcagg tccttgattc gaaggtgctt
     1801 ggaactgtgg agggagactg ttccttcctt catccctgat tcattcagga tcttgatccg
     1861 ccggtagaca tccacggtgg acttttcgag ggtctcgttg agcgcaatct gaatctcctc
     1921 ctccaggatg accgccccgg cgcccggagc gaagctgacc tcagtgagcg acttgtccgt
     1981 ctcagagatc tcattccaag gcgtccacgc tcgcgaggct gaaggaagcg cggtcagaac
     2041 cacaccgaga aggacactga cgcagaccgc gcgcatggag cggcaagtct accccgattg
     2101 acggaacggc cgcctacttg gcgggcgtcg gggcaccgcc ccgctcccga gcccggagca
     2161 cca
//
LOCUS       DASRJQ010000646         4116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3777862, whole genome shotgun sequence.
ACCESSION   DASRJQ010000646 DASRJQ010000000
VERSION     DASRJQ010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3777862"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..546
                     /locus_tag="VFB89_14605"
     CDS             16..546
                     /locus_tag="VFB89_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28575.1"
                     /translation="MTRRAATGLVITVLLTTAATAPLTAAQRLVDVRQVATDTLHDVA
                     VATYERGLPVIYYNPKLMQRIGPELADFFLAHEYGHIAYGHTGGALAEPRDELSALRQ
                     KQELDADCYATQTLAAENRPAVEAARQFFGRMGPFRFDNLHPSGSQRAAKILACLPAT
                     DRDETADLRNTARISH"
     gene            complement(602..898)
                     /locus_tag="VFB89_14610"
     CDS             complement(602..898)
                     /locus_tag="VFB89_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HZM28576.1"
                     /translation="MYAIALIRYRRQLDEVQTALDAHRAYLRSLQEKGLLIAAGPLDP
                     WTGGALLLRVPDSAPQAALDQIREGDPYTKQGFVQYEVWPWKPVLGKEALDGLQ"
     gene            947..1462
                     /locus_tag="VFB89_14615"
     CDS             947..1462
                     /locus_tag="VFB89_14615"
                     /inference="COORDINATES: protein motif:HMM:NF019241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HZM28577.1"
                     /translation="MGSNTGAAYLAEALRVLRQYKAMADRAVAQLEPEDIFRAPDDES
                     NSIAIIMQHMAGNMRSRWTDFLTTDGEKPTRNRDAEFDLAPGTSFAAVKQSWEDGWNV
                     TLAAIEALTPADLEREVRIRNEPHTVLQAISRQLAHYAYHVGQIVLLAKHYRGAAWQT
                     LSVPKRKPAVS"
     gene            1526..2092
                     /locus_tag="VFB89_14620"
     CDS             1526..2092
                     /locus_tag="VFB89_14620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02265 family protein"
                     /protein_id="HZM28578.1"
                     /translation="MQQVKGAVLKSRLAFVEEHFGKDAVQKVLASLAADDQRPLRLVF
                     TSNWYPFELGKRLDDAIVRVVANGRSDFFERLGAASAEKNLGSLHAGYLTPGDPQAFL
                     AKAPQIYSLYYETGRREYTATGPTSGVLTTYDAETFSEPDCMTVVGWYRKALEMCGAS
                     NVSVTEEECRARGGAVCRYKVLWKKREP"
     gene            complement(2166..2801)
                     /gene="pdxH"
                     /locus_tag="VFB89_14625"
     CDS             complement(2166..2801)
                     /gene="pdxH"
                     /locus_tag="VFB89_14625"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HZM28579.1"
                     /translation="MSLADLRREYAHARLDEREVDPDPMVQFRRWFDDARRAGLLEPN
                     AMTLATATPDGVPSARIVLLKAADELGFVFYTDYRSRKGQDLELNPRGALVFYWGELE
                     RQLRITGSVSRVSQQESEVYFQTRPRGSRIAAWVSEQSSVLSSRALLEDRIETMAARY
                     PGEQVPLPPHWGGYRLRPETIEFWQGRENRLHDRVRYQRQGDGWRIERLSP"
     gene            complement(2803..>4116)
                     /locus_tag="VFB89_14630"
     CDS             complement(2803..>4116)
                     /locus_tag="VFB89_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HZM28580.1"
                     /translation="SAQSTSNPNRPFGTLREQAARQQAWLKERLDSILPGLMRKYGID
                     MWVVPMREYNEDPVFTALVSPTTFAARRRTIYVFFDRGPSRGVERIALGGISQDGLYD
                     AVRSTKQVTASVGGRQAELWGDQQWKVLTDVIEARNPRVIGIDISRTFAFADGLSAGE
                     YEGMSQALGPKWTKRFKRAEGLPVDLIAARLPDEQRFFHDLAQLTWAVIDTMFSERVI
                     TPGTTRTSDLVWYFRQRVNDFGLGTWFQPSVDVQRRGVTDEQLGEDPVIERGDVLHCD
                     VGITALRLNTDIQHLAYVLQQGESLPPSGLVQALRRSNRLQDIVMEEIRAGRTGNAIL
                     VSARKRMTDEGIDGTIYSHPIGLNGHGAGPLIGLWDYQEGVPGRGDHRVIPDMWFSVE
                     LQATTPVAEWNGQPVRMAQEEDMVMGPDGTPRWAYRRQTELHLVH"
BASE COUNT          771 a         1333 c         1289 g          723 t
ORIGIN      
        1 agagtcggag ttacaatgac acggcgtgct gctacaggcc tggtgatcac cgtgctgcta
       61 acgacagcag ccacggcgcc gctgacagcc gcccagcgac tggtggacgt gcgccaggtg
      121 gcaactgata ctctccacga cgtcgccgtc gcgacctacg aacgcggcct cccggtcatc
      181 tactacaatc cgaaattgat gcagcggatc gggccggagc tggccgactt tttcctggct
      241 cacgagtacg gtcacatcgc ctacggccat accggcggcg cgctggccga gccccgtgac
      301 gagttgagcg cgctgcggca gaagcaggag ctcgacgccg attgctacgc cactcagacc
      361 ctggcggctg aaaaccgccc ggcagtagaa gcggctcgtc agttcttcgg ccgcatgggc
      421 cccttccgat tcgacaacct ccacccatcc ggcagccagc gagcggccaa gattctcgcc
      481 tgcctacccg ccaccgaccg cgacgaaacc gcggacctcc gaaacaccgc tcgaatcagc
      541 cattgaacct ggccgggtcg caggccgcgg ctcggccacc tactttcgat cgcgagtcgc
      601 gctattgtag tccatccaat gcttccttcc ccagcaccgg cttccagggc caaacctcgt
      661 actgcacgaa tccctgctta gtgtacggat cgccctcccg aatctgatcg agtgcagctt
      721 gaggtgcact gtcgggaaca cggagcagca aggcgccgcc ggtccaggga tcgagtggtc
      781 cggccgcgat caggagccct ttctcctgga ggcttcgcaa gtaggcccga tgcgcatcca
      841 gcgcggtctg cacctcatcc aattggcgac ggtagcggat cagggcgatc gcgtacattt
      901 cggactccgg gcgtagggtg aacggcgaag cgaggaggaa gtgtacatgg gatcgaacac
      961 aggtgcggcg tacctggccg aagcattacg agtcttgcgg cagtacaagg ccatggcgga
     1021 tcgagcggtg gcgcaactcg agcccgagga catcttccgg gcgcccgatg acgagtccaa
     1081 cagcatcgcc atcatcatgc agcatatggc cggcaacatg cgatcgcgct ggaccgattt
     1141 cctcaccacc gacggggaga agcccacccg taatcgggac gccgagttcg acctcgcacc
     1201 cgggacgtcg ttcgccgcag tgaaacagtc ctgggaggat ggttggaatg tcacgttagc
     1261 cgcaatcgaa gcgcttacac cggcagacct cgagcgggag gtcaggattc ggaacgagcc
     1321 gcacacggtg ttgcaggcca tctcgcgcca gctggcccac tacgcatacc acgtgggcca
     1381 aatcgttctt ctggccaagc actaccgggg cgccgcgtgg cagacgctga gcgtgcccaa
     1441 acggaagcca gcggtctcgt gaactgtggg cctgacctga gccccccggt actttagctt
     1501 tcgccgtcat cgcgccggag cgtgcgtgca acaagtcaaa ggagcggtgc tcaagtcccg
     1561 gcttgcgttc gtggaagagc atttcgggaa ggatgccgtc cagaaagtgc tcgccagcct
     1621 ggccgcggat gatcagcgtc ccctgcggct cgtcttcacc tcgaactggt accctttcga
     1681 gctggggaag cggctcgacg acgcgatcgt ccgggtggtc gcgaacggtc gctcggactt
     1741 cttcgagcgg ctgggcgcgg cgtcggctga gaagaatctg gggtcgcttc atgccggcta
     1801 tctcacgccg ggagatccgc aggccttcct ggccaaagcg cctcagattt actcgctcta
     1861 ctacgaaacg gggcggcggg agtacaccgc gacaggaccg acgtcggggg tgttgaccac
     1921 gtacgacgcc gaaacattca gcgaacccga ctgcatgacg gtcgtgggat ggtaccggaa
     1981 ggcgctggag atgtgcgggg caagcaacgt cagcgtgacc gaagaggagt gccgggcgag
     2041 gggaggagcg gtctgtcggt acaaggttct atggaaaaag cgtgaaccgt gaacagtgaa
     2101 cggtgaacgg aggtggcgcg ttcgaggagt cccctgttca ccgttaccgt tcacgtttta
     2161 cgctctcaag gtgacagacg ctctatcctc caaccgtccc cctgtcgttg ataccggact
     2221 cgatcgtgca gccggttttc ccttccctgc cagaactcga tagtctcggg ccgcagccga
     2281 taaccgcccc agtgcggcgg cagtggcacc tgctcgccgg gatagcgcgc agccatggtt
     2341 tcgatcctgt cttccaacag cgcgcggctg gagagcacgg agctctgctc ggaaacccag
     2401 gcggcaatgc ggctccctcg cggccgggtc tggaaataca cctcgctctc ttgctgcgat
     2461 acgcggctca ctgagccggt gatccgtaac tggcgctcca gttctcccca ataaaaaacc
     2521 agcgcgccgc ggggatttag ctcgaggtct tgccctttcc ggcttcgata gtcggtataa
     2581 aagacgaagc ccagttcgtc ggcggctttg agcaacacga tgcgagcgga cggcacaccg
     2641 tccggtgtcg ccgtggcgag cgtcattgcg ttgggttcga gcaggccggc ccgtctcgcg
     2701 tcatcgaacc accgacggaa ttgcaccatc gggtcgggat ccacctcccg ctcatcgaga
     2761 cgggcatgag cgtattcccg tcggagatcg gccagcgaca tgtcagtgca cgaggtgcag
     2821 ctcggtctgg cgccgatagg cccagcgcgg ggttccatcc gggcccatga ccatgtcctc
     2881 ctcctgcgcc atgcggaccg gctggccatt ccattcggcg accggcgtcg tcgcctggag
     2941 ctcgacggaa aaccacatgt cggggatgac gcgatggtcg cctcgacccg gcacgccttc
     3001 ctggtagtcc cacagaccga tcaggggacc ggccccatgc ccgttgagtc cgatcggatg
     3061 cgagtagatc gtcccgtcga tgccctcatc cgtcatccgt ttgcgagccg aaacgagaat
     3121 ggcgttgccg gtgcggcccg cgcggatctc ttccatcacg atgtcctgca ggcgattcga
     3181 ccggcgcaac gcctggacca ggccggacgg tgggaggctt tcgccttgct ggagcacata
     3241 cgcaagatgc tggatgtcgg tattgagccg gagcgccgta attccaacgt cgcaatggag
     3301 cacgtcgccc cgctcgatca cgggatcctc gcccaactgc tcgtcggtca ccccccgacg
     3361 ttggacgtcc actgatggct ggaaccaggt acccaagccg aagtcattca cccgctgccg
     3421 gaaataccag accaggtcgc tggtacgagt ggtgcccggc gtaatcacgc gctcggaaaa
     3481 catcgtgtcg atgacggccc aggtcagctg tgccagatca tgaaagaagc gttgctcatc
     3541 cggcagccgc gcggcgatca ggtctaccgg caggccttcg gcacgcttga agcgcttggt
     3601 ccacttgggg ccgagcgcct ggctcatccc ttcgtattcc ccggcactca ggccgtcggc
     3661 aaaggcgaag gtgcgcgaga tgtcgatccc gatgacccgc gggttgcgag cttcgattac
     3721 atccgtgagc accttccact gctggtcacc ccacagctcc gcctgccgtc cgcccaccga
     3781 cgcggtcacc tgcttggtgg agcggaccgc atcatacaga ccgtcctgcg agatgccgcc
     3841 cagcgcgatg cgctccacgc cgcgagatgg accccgatca aagaagacgt agatcgtgcg
     3901 ccgccgcgcg gcgaatgtgg tcggggaaac cagggcggtg aagacggggt cttcattgta
     3961 ctcgcgcatc ggcaccaccc acatgtcgat gccgtacttg cgcatgaggc ccggcaaaat
     4021 gctgtccagc cgctccttga gccaggcttg ctggcgcgct gcctgctcgc gaagcgtgcc
     4081 gaacggacgg ttgggattcg acgtgctctg ggccga
//
LOCUS       DASRJQ010000647         4973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1804748, whole genome shotgun sequence.
ACCESSION   DASRJQ010000647 DASRJQ010000000
VERSION     DASRJQ010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1804748"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VFB89_14635"
     CDS             complement(<1..345)
                     /locus_tag="VFB89_14635"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZM28581.1"
                     /translation="MSHLNHLNMSLRKLAGRAGVTTPTVYAYFESKNAILDAMFGEAA
                     ESFEQSNAEPVGDGNPHQQLVAHAHRFAAFCTSDVVRYQLLFQHLIPGFSPSPESYAP
                     AVRALDAARQRLE"
     gene            complement(361..729)
                     /locus_tag="VFB89_14640"
     CDS             complement(361..729)
                     /locus_tag="VFB89_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28582.1"
                     /translation="MSTFSELKEQAEQEIRAHIPSRSSDSTHHFVLDSTNYFVDHGAG
                     LLAALRVWGEFRRKTDADLRALIDSADAATLENIAEVLAHSHDPDELHASVQLEAVAF
                     APPPQLHATLDFARKGIEPS"
     gene            complement(908..1150)
                     /locus_tag="VFB89_14645"
     CDS             complement(908..1150)
                     /locus_tag="VFB89_14645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28583.1"
                     /translation="MPSEPNSSGEEELSEYVRRPTRTAGDLSEVAEAAAGITTEDLAR
                     DLETAASKQGALTDKELLADLLERVKRIEEHLGLTS"
     gene            1594..2115
                     /locus_tag="VFB89_14650"
     CDS             1594..2115
                     /locus_tag="VFB89_14650"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28584.1"
                     /translation="MFATARTLQRGTASAAGLAALTLALAACGASGSAAYGAGGYGAA
                     TTAPSAAAMAATVDLHNSSLGPILVDAQGKTLYLFEADKNGKSACSGPCATAWPPLLS
                     NGAPQAAMGASASLIGTTARSDGGSQVTYAGHPLYYYVGDKAAGDVTGQGIDQFGAKW
                     YVIGKDGKKIDND"
     gene            complement(2145..>4973)
                     /locus_tag="VFB89_14655"
     CDS             complement(2145..>4973)
                     /locus_tag="VFB89_14655"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZM28585.1"
                     /translation="GGCIELGEGSIPYGPLIQALRGLGRGLAPAALVDLVGAERAVLA
                     RMLPELGPSEHADEATQTPLTVGASSQARLFEAFLALLERLAERSPTVLVIEDLHWAD
                     RSTLDLLTFLVRNVRTGLLLVLTYRTDDLHRRHPLRPVLAELERSQRVERLDVDRFSR
                     AELATLLEAKLGRSLADDLVERIYRRSGGNAFFAEELLDAFHGREGDAHLPPSLENVL
                     LSRVQVLSDDAQATLRIVASASGPVEHELLAAVSDLPEPELLAALRAAVAHQVLVPDP
                     DSETYAFRHALTQEALYGDLLPGERAQLHAAFARALTEHPGVGDPAHSAARLAFHWVR
                     AHDPAHALPAAMEAGLQSQAAYGFADARRHFEMVLELWDQVAEPEERLGHDRGTVLRH
                     AAESAYLSGDPTRAIALTRAALAAVDEAGDPVRAGLLHALLGSYLNSSGGHGAIGEYE
                     QAVRLVPAHPMRPERAQVLAALGEALMAQGRYRESRELCEEALAIAQELGAFQQEGDA
                     RRSLGVDRAFLGDLEAGTRELREARRIAEALGRVDEVARCFATLSGLLEAFGELEAAA
                     TVALEGAELAATQGLGRWHSPFLTATAGRALFGLGRWDEADILLGRAADRVAPELLAA
                     RVSICSARVQLDVSRGRAESAAECLTEAREAYLHTVKQPWFAAPLFAATAELALLDGR
                     LADANAAVTEGLQVAGGDLRSAAPLYVLGVRTAAERAELARAHRNQHEVSDARERGNA
                     LCLELQTRMSPDESGGAVPTPPTHAYTAVGEAELARLEGRSHAGLWAAAALVWEGLAQ
                     PYPAAYARWREAEARLLAGAPRERVEALLSDAHATASSLAARPLCAEIEGLARRGRLN
                     LRTTAAVPITSEQPSPLTQLGLTAREQEVLALVATGLTNRQIAETLFISPKTATLHVS
                     NILSKLGVTNRVAAAAIAHRLGVASRNA"
BASE COUNT          900 a         1602 c         1619 g          852 t
ORIGIN      
        1 ctcgagacgc tgccgagcgg cgtcgagagc gcggacggcc ggggcgtagg actctggtga
       61 gggactgaag cctggaatca gatgctggaa cagcagctgg taccggacta cgtcggacgt
      121 gcagaaggcc gcaaaacggt gggcgtgggc taccagctgc tgatgcgggt ttccgtcccc
      181 aacgggctcg gcgttgcttt gttcgaagct ttcggcggcc tcaccgaaca tggcgtcgag
      241 aatggcgttc tttgactcga agtaggcgta cacggttggg gtggtgaccc ccgcgcggcc
      301 agcgagcttt cgtagcgaca tgttcaaatg atttaggtgg gacatcgatc cgaggcctta
      361 tcagctcggt tctatgccct ttcgtgcgaa gtcgagtgtc gcgtgcagct ggggtggagg
      421 tgcgaaagca accgcttcaa gctggacgct ggcgtgaagc tcatcaggat cgtgactgtg
      481 ggccagcacc tcggcaatgt tctccaacgt cgctgcatct gcggaatcaa tgagcgctcg
      541 aagatcggca tcggtctttc gccggaactc tccccagacc ctgagcgcgg cgagtaatcc
      601 cgctccgtgg tcaacaaagt agttggtgga gtccagtacg aagtggtgcg tggagtctga
      661 agacctggac gggatgtgcg ccctgatctc ctgctccgcc tgctccttga gctcactgaa
      721 agtgctcacg ctctagcagt gttccaccac ccgccttggg cgctcacaag gccttcgtca
      781 tccagtcccc tggatgcggc cgacaaagtc gctggcgtcg tcacggataa tctgtcggtg
      841 ctttccggct caggcgcgcg cttcgagcag cccttgaatg gtgggtgagc ccgagcctgg
      901 ccctcaccta actcgtgagt ccgaggtgct cctcgattcg cttcacccgc tcaagcaggt
      961 ccgcgagcaa ctccttgtcg gttagagctc cttgtttaga ggcggcggtt tcaagatcac
     1021 gagcaaggtc ttccgtcgtg attcccgctg cggcttcagc gacctcggac agatcgcctg
     1081 ccgtcctggt cggtcgacgg acgtactctg ataactcttc ttcccccgag ctgttcggct
     1141 cagatggcac tggttcctac gctcctcggt ggcggtacgg gcggcaggct gatccggttg
     1201 tcgaggttca acctaggcga cgagtgcaaa ggtccctgag ctcgtcaagg aataaactca
     1261 caggcgcgtc gtcgatcaac agctcggcat gggggaataa ttcctcagct ttcttgatcg
     1321 cgtaattcat aggccggtag cccttgcctg aacgccgcat gcaccgcccg cgttcggagc
     1381 aagatcaagt tcagaggggt tcagctcaga aggagtccag ctcatcacgg gagtccgtag
     1441 gagtatcgcc gccaagaact aggggtacgt ggaagcccag tactggcgca ttgtccgatt
     1501 tgtgactcac aagatcgccg tagcgtgcga actagagcgg cccgtccacg gtctaacaat
     1561 tcgaaggccc acaccccaag ggagcacaac aacatgttcg caacagctcg caccctccag
     1621 cgcggcactg cgtcggccgc cggcctggcg gcactcactc tcgctctcgc cgcatgcggg
     1681 gcttccgggt ccgcggccta cggcgccggc ggctatggcg cggccaccac cgcccccagc
     1741 gcagcggcga tggccgctac cgtcgacctg cacaattcca gcctcgggcc gatcctcgtc
     1801 gatgctcaag gcaaaacgct gtatctgttc gaggccgata agaacggaaa gtccgcctgc
     1861 agtggcccat gcgcgaccgc ctggccgccg ctccttagca acggcgcgcc gcaggcggcc
     1921 atgggtgcaa gcgcaagctt gatcggcacc acggctcgca gtgatggcgg gagtcaggtc
     1981 acctacgccg gtcacccgct ctactactac gtcggagaca aagctgccgg cgatgtgact
     2041 ggacaaggca tcgaccagtt cggcgccaag tggtacgtca tcggcaagga cggcaagaag
     2101 atcgacaacg actaaaagac taaaggatgc cttttctctt ctgtctaggc atttcgggac
     2161 gcgacaccga gccgatgagc gatcgcggcc gccgccaccc gattggtgac gccgagcttg
     2221 ctgagaatgt tggagacgtg caatgtggcc gtcttcgggc tgatgaaaag cgtctcggcg
     2281 atctgacggt tggtcagacc cgtcgccacc aaggccaaaa cctcctgctc acgagctgtg
     2341 agaccgagct gggtgagcgg cgacggctgc tctgaggtga tcgggaccgc agcagtggtt
     2401 cggaggttga ggcggccgcg gcgggctaag ccctcgatct cggcgcacaa cgggcgggcg
     2461 gccagactgc ttgcggtagc gtgcgcgtcg ctgagtaagg cttctactcg ttcccgggga
     2521 gcacccgcga ggagccgcgc ctccgcctcg cgccaccgcg catatgcggc cggatagggc
     2581 tgggcgagcc cctcccagac cagagctgcc gctgcccaaa gaccggcatg gctgcgcccc
     2641 tcgagccgcg caagctccgc ctcgccgaca gccgtgtacg cgtgggtcgg cggtgtcggc
     2701 accgcgccgc cggattcgtc gggtgacatc cgagtctgta gctcgaggca caacgcgttt
     2761 ccccgttcgc gggcgtcgga cacttcgtgc tgattgcgat gggcgcgggc cagctccgcg
     2821 cgttcggcgg cggtccgcac tcctaacacg tacaggggtg ccgcagagcg gaggtcgccg
     2881 ccagccacct gcaatccctc ggttaccgca gcattggcgt cggccagtcg gccatctaac
     2941 agggccaatt ctgccgtcgc agcgaacagc ggcgccgcga accagggctg cttgacagtg
     3001 tgcagatagg cctctcgagc ctcggtgagg cactcggcgg cggattcggc acgtccccgg
     3061 ctgacatcga gctggacgcg ggccgagcag attgaaacgc gggccgcgag gagttcgggg
     3121 gccacccgat cggcggcacg ccctagcaaa atgtcggcct cgtcccagcg acccagtccg
     3181 aagagcgctc ggcccgcggt cgcggtcaag aacgggctgt gccaccgccc caatccttga
     3241 gtcgctgcca gctccgcgcc ctccaaagca accgtcgccg cggcctccag ctcgccaaac
     3301 gcttcgagca gcccagacag cgtcgcgaag cagcgggcga cctcatcaac tcggccgagt
     3361 gcttcggcga ttcgacgagc ctcgcgcagt tctctagtgc ctgcctcaag atcaccgagg
     3421 aacgcacggt ccacgcccag ggaccgtcgc gcgtcgccct cctgttgaaa ggcacccagc
     3481 tcctgggcta tcgccagcgc ctcctcgcac aattcgcggg attcccggta gcggccttgc
     3541 gccatcagcg cctcaccgag cgcggccaac acctgagcgc gctctggccg catcgggtgc
     3601 gccggcacaa ggcgtacggc ctgttcatac tccccaattg cgccgtggcc accgctcgaa
     3661 ttgaggtagc tgcccaggag tgcatgcagc aagccggccc gtacggggtc accggcctca
     3721 tcaaccgcgg ccagagccgc tcgggtcaag gcgatggcac gggtgggatc gccagaaagg
     3781 taggccgact cggccgcgtg ccgaagtacg gtgccacggt cgtggccaag ccgctcttcg
     3841 ggctcggcaa cttgatccca tagctcgaga accatctcga aatgccgccg agcgtcggcg
     3901 aacccgtacg ccgcttggga ctgcaacccg gcttccatgg ctgcgggcaa cgcatgagcc
     3961 gggtcgtggg ctcgcaccca gtggaaggca aggcgcgccg cgctgtgggc gggatcgccc
     4021 acgccggggt gctcggtcaa tgctcgagca aaggctgcat gcagttgagc acgctctccg
     4081 ggcagcaggt ccccatagag cgcttcctgg gtgagggcgt gccggaaggc atacgtctcc
     4141 gaatctggat cggggaccag cacctggtgc gcgaccgcag cgcgtaaagc ggcgagcagc
     4201 tcaggctcgg gcagatcgct tacagcggca agcagctcgt gctcgaccgg accgctggct
     4261 gaggcgacga tccgaagcgt tgcctgcgcg tcgtcggaca gcacttggac tcggctgagc
     4321 agcacgtttt caaggcttgg tgggagatgt gcatcgccct ctcgcccgtg aaaggcatcg
     4381 aggagttcct cggcaaaaaa cgcattgccg ccggagcggc gatagattcg ctcgaccaga
     4441 tcgtcggcga ggctgcggcc aagcttggcc tcgagcaagg tcgcgagctc ggcacggctg
     4501 aagcgatcga cgtcgagtcg ctcgacccgt tggctccgct cgagttcggc cagaacggga
     4561 cgaagcggat ggcggcggtg gagatcgtcg gtgcggtaag tcagtaccag tagtagcccc
     4621 gtccgcacgt tacggacgag gaaggtgagt aaatcaaggg tcgagcggtc ggcccaatgg
     4681 agatcttcga taaccagcac cgtcggcgac cgctccgcga ggcgctccag cagcgccaga
     4741 aatgcctcaa agagcctggc ctgagacgag gcaccgacgg tgaggggcgt ctgggtcgcc
     4801 tcgtcggcat gttcagacgg cccgagttct ggcaacatcc gcgcaagcac tgcgcgctcg
     4861 gcgccgacaa ggtcgaccaa tgccgctggc gcgagacctc ggccgagacc gcgcagagcc
     4921 tggatcagcg gcccgtacgg tatcgatcct tcgccgagtt cgatgcaccc gcc
//
LOCUS       DASRJQ010000648         3444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3495080, whole genome shotgun sequence.
ACCESSION   DASRJQ010000648 DASRJQ010000000
VERSION     DASRJQ010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3495080"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VFB89_14660"
     CDS             complement(<1..692)
                     /locus_tag="VFB89_14660"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HZM28586.1"
                     /translation="MTTHQLFGTSCYAHCFAFYGRVSTEDNQDPESSYNWQFMLAEKL
                     VIPVGGRIVANFFDIGDSRSIPWPRRSDGSRLLAALRDPNRGFDNVVIGEPHRAFYGN
                     QYGLTIPVFAHFGVRLWVPQVGGPIDPDNEAHDLIMSVFGGMSKGERTRVKVRVHSSM
                     AAQAQLQGRYLGGRPPYGYTLKDLGPHPNPQKAADGKRLYGLTPDPVTAPIVQRIFRD
                     FLNGNGIFAIAEA"
     gene            1168..1401
                     /locus_tag="VFB89_14665"
     CDS             1168..1401
                     /locus_tag="VFB89_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28587.1"
                     /translation="MGRLPSRYDRSAGRREYEAAVRELQQAMLRFEAAGVPLTTDLGR
                     MMQLWDREQVGAVVEIRDAWIQLVAIVAVLDTA"
     gene            complement(1598..2542)
                     /locus_tag="VFB89_14670"
     CDS             complement(1598..2542)
                     /locus_tag="VFB89_14670"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HZM28588.1"
                     /translation="MTDGDWRDHGFRQRRPSAETLAWVAASMGRGSRIIGYRRLTGGV
                     CSAVHRLAVERRGVRTFVVLRQYPGGLGLHKALEKEIANLGVVAGSGLPVPSILAADV
                     AGASTGGAPASLMTRLPGDVDLEPAEPQSWMTRIAEIAVLLHSLDLPAKIFSPWTDSW
                     IAPRDGFQMPAGAQKPEVWKAAFGVMAAPPPKDTAVFLHCDLLPVNLLWSRGKVTGLT
                     DWNSIHRGSRAIDVGHCRRYLAALYSPEWAEQLRSLYESIAGATLDPWWDLYALLHYD
                     DSGPKWIHGQVAGRRPVDVPGMTSRVEIAIQTALQRLG"
     gene            complement(2560..2709)
                     /locus_tag="VFB89_14675"
     CDS             complement(2560..2709)
                     /locus_tag="VFB89_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28589.1"
                     /translation="MVGVGGSLEAQSGEREHVDQPAGAAGDIVGAQNCIEFAVDGRAG
                     AAEVP"
     gene            complement(2945..>3444)
                     /locus_tag="VFB89_14680"
     CDS             complement(2945..>3444)
                     /locus_tag="VFB89_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28590.1"
                     /translation="TPPAALAVITDRAIDQLRMPQPVIAANPSLTAKQFTHLPVWWWV
                     QPGWWQTQTATASAGGLTITARATPKRITWYAGDGTSTVCNGPGTPWTGSAAPTSASP
                     TCGHTYTVPSASEANPDGKFQLRAVVTWDITWSGGGLSGSVPDITTATTTDITVTQLR
                     AVITG"
BASE COUNT          605 a         1019 c         1183 g          637 t
ORIGIN      
        1 gcttcggcga tggcgaagat gccgttgccg ttgaggaagt cgcggaagat gcgctgcacg
       61 atgggggcgg tcaccgggtc tggtgtcaga ccgtagagcc gctttccgtc ggcagccttc
      121 tgcgggttcg ggtgcggacc aagatccttg agtgtatagc cgtacggcgg tcgcccaccg
      181 aggtagcggc cctgcaactg tgcctgcgcc gccatggagc tgtgtacacg taccttcacc
      241 cgagtccgct cgcccttact catcccgccg aagaccgaca tgatcaggtc gtgggcttcg
      301 ttgtcgggat cgatggggcc gccaacctgc ggtacccaca gcctgacgcc gaagtgggcg
      361 aagaccggga tggtgaggcc gtactggttg ccgtagaagg cgcggtgggg ctcgccgatg
      421 actacattgt caaagccacg gttggggtct cggagggccg caaggagccg actgccatcg
      481 gatcgacgcg gccagggaat tgaccggctg tcgccaatat cgaagaagtt agcgactatt
      541 cgtccgccaa cgggtatgac gagcttttcg gcgagcatga attgccagtt gtacgaagac
      601 tcggggtctt ggttgtcttc cgtagagact cgcccgtaga atgcgaagca gtgcgcgtaa
      661 cacgatgttc cgaagagctg gtgtgtggtc attcagactc caatgtttgg ctgctctttt
      721 ggcttctgcg cttgtcgcgt acttcgagta gcagtcgaag taacgccttg gctgcaggcg
      781 gttcgagcgc tggtggttga tctggcacga ttacggacag gtcggcagaa ttgcgttgcg
      841 acctagttga attgtagctg tcattggttg atctacgccg ttcggattcg ctattcggca
      901 tcgttccccc agatggaatg gcggcgctct cgcgcgcggt gatttggcca tcgggtacct
      961 ccattcactt cgcgtgttgg catagaaaga gcccatcgag tcaatgctcg atgggctcgc
     1021 gactggtggg aattgcttac gtacaaagtg caaattcgtt gggcatagtc ggtgaattcg
     1081 gagccgctgc tccgacgagg ggcagtccat gagtgggccg cggacggcgg cacgggcttc
     1141 aaacaggtgt tcgattagga tctcgccatg ggtagactgc cgtcgcgcta cgaccgctcg
     1201 gcagggagac gggagtacga agcggccgta cgggagctgc agcaggctat gctccgcttc
     1261 gaggcggctg gcgtcccgct cacgaccgac ctcggccgga tgatgcagct gtgggaccgt
     1321 gagcaagtgg gcgcggtcgt cgagattcgt gacgcctgga ttcagctggt ggctatcgtc
     1381 gcggttctgg acaccgcttg aacgtcgctt tggacaccgc ttggtcgtcg gcggtttcgt
     1441 gtctgcgagt ttcgacacca cggtgccgca aggttgtcga gatcttggaa gtggtgagtt
     1501 ctgggccgcg gtgtggtggc cgtgactgag gtgcgttcgg tacggtcggg tggtgggtga
     1561 gcgccacgcg caaagggcga ccagcgggga gccgcggtta cccgagacgc tgtagcgccg
     1621 tttggatagc gatctcgacc cgggaagtca tgccgggcac atcgacggga cggcgccccg
     1681 cgacctgacc atggatccac ttcggcccgc tgtcgtcata gtgcagcagg gcgtacaggt
     1741 cccaccacgg atcgagggtg gccccggcga tcgactcata aagcgaccga agctgctctg
     1801 cccactccgg cgaatagagc gccgctaggt atcgccggca gtgcccgacg tcaatcgcgc
     1861 gcgacccccg gtggatggag ttccagtcgg taagaccggt aacttttccg cgcgaccaga
     1921 gcaggttgac cggcaggaga tcgcagtgca ggaagacggc cgtgtccttg ggcggcggcg
     1981 cagccatgac accgaacgcc gccttccaca cttccggctt ctgtgcgcca gccggcatct
     2041 gaaacccgtc ccgaggagcg atccaggaat ccgtccacgg actgaagatc ttcgcgggga
     2101 gatcgaggga atgcagcaat acggcaatct ctgcgatcct cgtcatccac gactgaggtt
     2161 ccgccggttc gaggtcaaca tctcccggca accgggtcat cagcgacgcg ggcgcgcccc
     2221 cggtcgaagc gccagcgaca tcggcagcca ggatgctcgg aaccgggagc ccgcttcctg
     2281 ccaccacacc gaggttggcg atctccttct cgagggcttt gtgcaggcca agtccgcccg
     2341 ggtactgccg cagcacgacg aacgttcgca ctccacgccg ctcgacagcc agccgatgca
     2401 cggcggagca gacaccaccg gtcaatcggc gatagccgat gatgcgactg cctcgaccca
     2461 tcgacgccgc aacccaggcg agagtctccg cggagggtcg acgttgcctg aacccgtgat
     2521 ccctccagtc gccgtcggtc atgagtgcga gcctattcgt caggggactt cggcagcgcc
     2581 agcccggccg tccaccgcga actcgatgca gttctgcgcg cccacgatgt caccggcggc
     2641 cccggctggc tgatcgacat gctcgcgctc acccgactgc gcctccaaac ttccgccgac
     2701 cccgaccaac ccccgacagc ggacggaagc gcgttcaaaa ctcgcggaga ctgatcaacg
     2761 gccgaaccga tcaatggagt caaactcacg agctgctggt gtcacaactc gcgagcagag
     2821 ccacctggtg gaaacgcgac gtacctacga gcacctctta cgcgacgacg gcgaatgacc
     2881 gccagaaaag tgagagccca ccgaggacgg gggacctcgg cgggctctgt gttttgcggt
     2941 cagtctagcc agtgatgaca gcgcggagtt gggtcacggt gatgtctgtt gtggtcgcgg
     3001 tcgtgatgtc cgggacggag ccggacagtc cgccgccgga ccaggtgatg tcccaggtga
     3061 cgacggcgcg gagctggaac ttgccgtctg ggttggcctc tgatgctgac gggacggtgt
     3121 aggtgtgtcc gcacgtcggc gaagccgagg tgggcgcggc ggagccagtc cacggcgtgc
     3181 cagggccgtt gcaaacggtg gaggtgccgt ctcccgcgta ccaggtgatt ctcttgggcg
     3241 tggcgcgggc ggtgatggtc aacccgccgg ccgaggcggt ggcggtctgc gtttgccacc
     3301 agccgggctg gacccaccac cagacgggga ggtgcgtgaa ctgcttcgcg gtcagggagg
     3361 ggttggccgc gatgacgggt tggggcatgc gaagttggtc gatggcgcgg tcggtgatga
     3421 cggcgagcgc ggccggtggc gttg
//
LOCUS       DASRJQ010000649         2930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_963908, whole genome shotgun sequence.
ACCESSION   DASRJQ010000649 DASRJQ010000000
VERSION     DASRJQ010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963908"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VFB89_14685"
     CDS             <1..666
                     /locus_tag="VFB89_14685"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28591.1"
                     /translation="LLRIAEIAERFGGEIRLTREQNFIITHVPSDAVAVTVAELARIG
                     LPLGLNGMRATSVACTGSPLCNFAVSLTKPRLVEIMDRLEASFGKQVEDLRINLDGCP
                     HACAQHWIGDIGLQGTTLRERTPEGEKQEAYDLYLRGGQGVNAAIGVPLVRRVPAPEV
                     GAHVERLVRVWLDERSESESFKSFADRQPDERLVAVAADVSIDEARSRLRRRDSRAEV
                     VDV"
     gene            659..1468
                     /locus_tag="VFB89_14690"
     CDS             659..1468
                     /locus_tag="VFB89_14690"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HZM28592.1"
                     /translation="MSEAVARILPADEPELLDDVEAGELAVEFDDKPPEELLAWGLER
                     FGDRIALCTSFQAEGMVLLDMAWRLDPKVRVFTLDTGRLHQETYDIIDTVRKRYQLEI
                     EVHSPDTRELEAMTRKHGVNLFYESVPQRLLCCQVRKVRPLQRALNGLDAWITGLRRE
                     TWASRADIRKIELDHEHGGIAKLNPLADWTHDEIWDYLRANEVPYHPLYDQGFTSIGC
                     APCTRRTAPGEDPRAGRWWWEKNAPKECGMHCTIETGGFEHELEVLVERSR"
     gene            1452..1910
                     /locus_tag="VFB89_14695"
     CDS             1452..1910
                     /locus_tag="VFB89_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28593.1"
                     /translation="MSVRVEEPAREALAVEAAALSEAVSDPETSAGYADLSRALVDGE
                     VGDPLLPPLERLLELTLRTGRVRRLHGPHAEAAAVRLYQATPAGRALGSSLSEVNDAL
                     AALHGRALDGVSFTSKGPGAYGLAIRTGDAQISLEITPEGIAVRDVSVGE"
     gene            1912..>2930
                     /locus_tag="VFB89_14700"
     CDS             1912..>2930
                     /locus_tag="VFB89_14700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZM28594.1"
                     /translation="MTPPSRPYYYPVLLDLRNRRAVVVGGGTVAEGKVGPLLDAGAAV
                     TVVAPVLTPGLSLRAREGRLVHVGRSYQPGDLTDAHLVIAATDDPEVNHAIHAEAESQ
                     RIPVNVVDDPPYCGFILPSILRQGDLVVAVSTSGNAPALAVRIRERLERELGDEYGRF
                     LELAGSIRAPLADRFPDFQIRKRLWYRLVDSDVLALLRAGEEDRAWQRITEIMGVEVP
                     PRSAQHVGKEDGTRAAGNIARPSSGTKVVGTVYLVGAGPGDPKLITARGLEVLRRADV
                     VVYDRLVSHALLDEAPADAELVYAGKAPGGHCMPQEGINALLVREARLGKSVVRLKGG
                     DPFVFG"
BASE COUNT          509 a          908 c         1029 g          484 t
ORIGIN      
        1 ctgctccgta tcgccgagat cgccgaacga ttcggcgggg agatccgcct cacgcgggag
       61 cagaacttca tcatcacgca tgtgcccagc gatgctgtgg cagtcactgt tgccgagctg
      121 gcgaggatcg gcctcccact cgggctcaat gggatgcgcg ccacaagcgt tgcctgcacc
      181 ggctcgcctc tctgcaactt cgccgtgtcg ctcaccaagc ccaggctggt cgagatcatg
      241 gatcggctgg aggcctcgtt tggcaagcag gtcgaggacc tccggatcaa tctcgacggc
      301 tgtcctcacg cctgcgccca gcactggatc ggcgatatcg gcctccaggg aacgacgctg
      361 cgcgagcgga cgccggaagg cgagaagcag gaagcctacg atctctatct gcggggaggg
      421 cagggggtga acgccgcgat cggagtgccg ctggtccgcc gagtgccagc gccggaagtg
      481 ggcgcgcacg tggagcgctt agtgcgggta tggctggacg agcggtccga gtcggaatcg
      541 ttcaagagct ttgccgaccg tcagccggac gagcggctgg tcgcggtggc ggccgatgtg
      601 tcaatcgatg aggcgcggag ccggcttcgt cggcgcgata gccgcgcgga ggtggtcgat
      661 gtctgaggca gtcgcgagga tcctgccggc ggatgagcca gagctgctcg acgatgtcga
      721 agcgggagag ctggcggtcg agttcgacga caagcccccc gaggagctcc ttgcctgggg
      781 actcgagcgg ttcggcgacc gtatcgcact ctgcaccagc tttcaggccg aaggcatggt
      841 tctcctcgac atggcctggc ggctggatcc gaaggtgcgg gtcttcacgc tcgataccgg
      901 ccgattgcac caggagacct acgacatcat cgacaccgtc cgaaagcgct atcaactcga
      961 aatcgaggtg cattcgcccg atacccgcga gctggaggcg atgacccgga agcacggggt
     1021 caacctgttc tacgagtcgg tgccgcaacg gctcttgtgc tgccaggttc gcaaggtgcg
     1081 gccgctgcag cgggccctga acggcctcga cgcatggatc acggggctca ggcgtgagac
     1141 ctgggccagc cgcgccgaca tccggaagat cgagctggac cacgagcacg gcgggatcgc
     1201 caagctcaat ccactggccg actggaccca cgacgagatc tgggattacc tgcgggccaa
     1261 tgaagtcccc taccatccgc tctacgatca gggcttcacc agtatcggat gcgccccgtg
     1321 cacccggcgc accgcacccg gcgaggatcc tcgcgcaggg cgctggtggt gggagaaaaa
     1381 cgcccccaag gagtgcggga tgcactgcac catcgaaacc ggcgggttcg agcatgaatt
     1441 ggaggtgctg gttgagcgtt cgcgttgaag agcccgcgcg cgaagcgctc gctgtggaag
     1501 cggcggcact cagtgaggcg gtaagcgacc ctgagacgag tgccgggtac gccgatctct
     1561 ctcgggcgct ggtcgacgga gaagtcggcg atccgctgct gcctccgctg gaacggctgc
     1621 tcgagctgac gttgcggacc ggtcgcgttc gccgactgca tggaccgcac gcggaggccg
     1681 cggcggtccg cctctatcag gcgaccccgg cagggcgcgc gctcggcagc agtctttccg
     1741 aggtcaacga cgcgctggct gcgttgcatg gacgtgcgct cgacggcgtc agcttcacat
     1801 ccaaggggcc gggagcgtac ggcctcgcca ttcgaaccgg cgacgcacag atctcgctcg
     1861 agatcacacc cgaaggcatc gcggttcgag atgtaagcgt gggtgaatga gatgacgccg
     1921 ccctctcggc cctactacta ccctgtgctg ctcgacctcc gtaatcgacg ggccgtggtc
     1981 gtcggtggtg gaaccgttgc cgaggggaag gtcggtccgc tgctggatgc gggtgccgcg
     2041 gtgacagtgg tcgcacccgt gctgacgccg ggcctctcgc tacgcgcgcg cgagggtcgc
     2101 ctggtgcatg tgggtcggag ctaccaaccg ggggacctca cggatgctca cctcgtgatc
     2161 gccgcgaccg acgatcccga ggtgaaccac gccatacatg cggaggcgga gagccagcga
     2221 attccggtaa acgtggtgga cgatccaccg tattgcggct tcatcctgcc ttcgatcctg
     2281 cgccagggag acctggtcgt cgcggtctcg acctcaggca acgccccggc tctcgctgtt
     2341 cgcatccgcg agcggctgga gcgagagctg ggcgatgagt acggacgatt cctcgagctg
     2401 gcaggctcca ttcgcgcccc cctggccgac aggtttccgg actttcagat ccggaagcgg
     2461 ctctggtacc gcctggtaga ctcagacgtg ctggcgctcc ttcgcgcagg agaggaggac
     2521 cgggcttggc agcggatcac ggagatcatg ggcgtagagg ttcccccgcg atcggcccag
     2581 cacgtaggga aagaggacgg aacgcgcgcg gcggggaaca tcgccagacc atcgagcggg
     2641 acgaaggttg tcggaacggt atacctggtc ggcgccggtc cgggcgaccc caagctgatc
     2701 accgctaggg gcctagaggt gcttcggcgt gccgatgtcg tcgtctacga ccggctggtg
     2761 agtcatgcgt tgctggacga ggcgccggcc gatgccgaac tggtgtacgc aggtaaggcg
     2821 ccgggcgggc attgcatgcc gcaggaaggg atcaatgccc tgttggtgcg cgaagccagg
     2881 ctgggaaagt cagtcgtgcg actcaaaggc ggtgatccct tcgtcttcgg
//
LOCUS       DASRJQ010000650         2363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2850400, whole genome shotgun sequence.
ACCESSION   DASRJQ010000650 DASRJQ010000000
VERSION     DASRJQ010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2850400"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VFB89_14705"
     CDS             <1..430
                     /locus_tag="VFB89_14705"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZM28595.1"
                     /translation="VLMERQHEAVTAMHRLRGIGVQLSIDDFGTGYSSLSYLKHFPVD
                     TLKIDRTFVRDLMTVPDDAAITAAIIAMAHALELKVVAEGVETHEHLAFLRGQGCDEI
                     QGHLVGRAVTAERFAEWLARSRNPGGRIGAAGSRINRAAG"
     gene            complement(427..1011)
                     /locus_tag="VFB89_14710"
     CDS             complement(427..1011)
                     /locus_tag="VFB89_14710"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28596.1"
                     /translation="MKRLAVVKLGGGLARAPGALARASAAIEKAAQRKRLLIIPGGGV
                     LADAVRLAERELGLTPTAVHWMAILAMDQFAYAIASAIPGSRVVHDAAEIQNALADSV
                     VPVLAPYRWMLAADVLPHGWHATSDSIAAFIAGALDAECLVLLKPVSGPVEALVDPCF
                     RDVLPVGLTVLVLGAEEIERLPRILQESRSKAVD"
     gene            complement(1008..2039)
                     /locus_tag="VFB89_14715"
     CDS             complement(1008..2039)
                     /locus_tag="VFB89_14715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HZM28597.1"
                     /translation="MGSIIGWDIGGVNIKAARFVAPDKPRAVLEPFELQRAPARLAPT
                     LKVIAARLEAQSDDTHAVTMTAELSQYFRTKREGVAFVLDALDTALVGAPLHVFGTDG
                     VFRSVDRARADALSVAAANWAATAHLVARTSGTCLLIDIGSTTTDIVPIVDGVVTVVG
                     RTDPERLASGELVYTGALRTPTEAVAHHVSLRGERAGVSAEGFATMGDVYLWLGSLAP
                     EDYNIPTPDGRPATREFAGERLARVVCGDREILGDADIDVIATELAAAQLAGIIGAIR
                     RVRARHYRIAEAVVTGLGDFIVAEAAERAGLTYVRLADRLGEDGARAAPAAAVAILLN
                     ESIALGRSS"
     gene            2064..>2363
                     /locus_tag="VFB89_14720"
     CDS             2064..>2363
                     /locus_tag="VFB89_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28598.1"
                     /translation="MEIIPVLDVTMGVAVHAQGRVRADFAPVKSVLAPDQPGDPLALA
                     RAYRDVLGAQACYLADLDAIQGGPIQRGLIRDLVEFQTGFAGELMVDAAAADPDGA"
BASE COUNT          421 a          766 c          774 g          402 t
ORIGIN      
        1 cgtattgatg gaacgtcaac acgaggcggt gaccgcgatg catcggctgc gcggaatcgg
       61 ggtgcagctt tccatcgacg attttggcac cgggtattcg tccctgagct atctgaagca
      121 ctttccggtc gacaccttga agatcgaccg taccttcgtt cgcgatctga tgacggtgcc
      181 ggacgatgcg gccattactg ccgccatcat cgctatggcc cacgctctgg agttaaaggt
      241 ggttgcggag ggggtggaga ctcacgagca cctggcattc ctgcggggac agggctgcga
      301 cgaaattcag gggcatttgg tcggacgggc agtcacggcg gagcggtttg ccgaatggct
      361 ggctcgcagc cggaacccgg gcggccggat tggtgctgcc ggcagccgca ttaatcgcgc
      421 ggccggctag tccaccgctt tcgagcgaga ctcctggagt atcctcggaa ggcgctctat
      481 ctcctccgca cccagcacca gcaccgtcag ccctaccggc aacacgtccc gaaagcacgg
      541 gtcgaccaat gcctcgaccg ggccactcac cggcttgagc agcacaagac actcggcgtc
      601 gagcgctccc gcaatgaacg cagcaatgct gtcactggtt gcatgccagc cgtgcggcag
      661 cacgtccgct gccaacatcc agcgatacgg agcgagaacc ggcacgacgg aatccgccag
      721 ggcgttctgg atctcggcgg catcgtgtac gacgcgcgag ccgggaattg ctgatgcgat
      781 ggcgtatgcg aactgatcca tcgcgagaat cgccatccaa tgtaccgcgg tcggtgtgag
      841 tcccagttcc cgctccgcca ggcgcactgc atcggccagc actccgcctc caggtatgat
      901 cagcagccgc ttccgttgag cggccttctc aatagcagcg gacgctcggg ccagggcgcc
      961 cggtgcgcgc gcgagcccgc cacccagctt gacgacggct agacgtttca cgagctccgg
     1021 ccgagcgcga tcgactcatt gagcaggatt gccaccgctg ctgcaggggc tgccctcgcg
     1081 ccgtcctccc cgagacgatc agcaagccgg acgtaggtga ggccggctcg ttcagccgct
     1141 tcggcgacaa taaagtcacc caggcccgtc acgacggctt ccgcgattcg gtagtggcgc
     1201 gcacggacgc gccggatcgc gccaatgatg ccggcgagct gtgcggcggc cagctcggtc
     1261 gcgatcacat cgatatccgc atcacccaga atctcccggt caccacagac tacccgggcg
     1321 aggcgctctc cggcgaactc ccgcgttgcc gggcgcccat ccggggtagg aatgttgtaa
     1381 tcttcaggcg caagcgaacc tagccagaga taaacgtctc ccatggtcgc gaacccttcg
     1441 gcagaaacgc cggctcgttc gcctcggagt gagacgtgat gcgcgacggc ttctgtgggt
     1501 gttcggagtg cgccggtgta aaccagctcg ccgctggcga gccgctccgg atcggtacgg
     1561 ccgacaacgg taacgacgcc gtctacgatc ggaacgatgt ctgtggtggt cgagccgata
     1621 tcgatcagca ggcacgtgcc ggaggtccgg gcaaccagat gcgcggtcgc cgcccagttc
     1681 gccgccgcga cgctgagtgc gtcagcccgg gcccggtcca ccgagcgaaa gactccatcg
     1741 gtgccgaaga cgtgcaatgg ggcgcccaca agagccgtgt ccagagcatc gagcacgaac
     1801 gcgacaccct cgcgcttggt gcggaagtat tgggacaact cggcggtcat cgtgacggca
     1861 tgggtatcgt ccgactgggc ttcgagccgt gcggcgatga cttttagggt gggcgcgagt
     1921 cgggccggcg ctcgctgcag ctcaaagggc tcgagcaccg cgcgtggctt gtctggggcg
     1981 acgaaacgcg cggccttgat gttgacgcca ccgatgtccc agccaatgat gcttcccatc
     2041 acctactgac cggcaggctg cgcatggaaa tcattccggt tctggacgtg accatgggag
     2101 tggccgtgca tgcacagggg cgagtccgag cggactttgc gccggtaaag tcggtactgg
     2161 cacccgatca gcctggcgac cccctggcgc tggcccgcgc ctatcgtgac gtgctgggcg
     2221 cccaggcctg ttacctcgcc gacctcgatg cgatccaggg cggcccgatc caacggggct
     2281 tgatccgtga cctggtagag ttccagaccg gcttcgccgg agagctcatg gtggatgcgg
     2341 cggctgccga tccggacggc gcg
//
LOCUS       DASRJQ010000651         2767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1240059, whole genome shotgun sequence.
ACCESSION   DASRJQ010000651 DASRJQ010000000
VERSION     DASRJQ010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240059"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..1783
                     /locus_tag="VFB89_14725"
     CDS             224..1783
                     /locus_tag="VFB89_14725"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HZM28599.1"
                     /translation="MAGASVVVAAGVVVFLAQWVSVALWVPPIRVSSVWLTGGVMLAA
                     ALLSQPRHWPAVIAAGATGQTFLFLGMQLVSPGAAILLGLLAALQTVAVALALRAVVR
                     GRYILSTLREFRDYGVVAIGGGAISASILFVAGAWSMRFRPATFLTWRTFALAAVLGY
                     LMVTPTLVLLVQRIMHPARGDPRRRFEGSLLSVLLTVVSGSVIVLAERGSLAWPVLVA
                     TIPPLLLWAVVRFGALGASASLLVVALVSSLGAAHGLTVFAGRTQADHILFLQLFLLG
                     TGVPLLGMGVVMDEQKRTTNALQSTRGRLQKLNRDLMKAREAEATRIARELHDDVGQR
                     LALISIGLSRLRRSDAQRTGAEIRRLQEQTGSVVRSLRDLSHQLHPAVLEHAGLPLAL
                     QMKCEEVFQATGIEVRLVSHGETSEIPPDIALCLFRVAQEALSNVIRHSGAHLVDLSL
                     DREGSELRLKVRDDGRGFTPGAARNGNGLGLYHVTERLGAFGGSLALESAPGAGTTLR
                     VAVPLNGVHDA"
     gene            1776..2408
                     /locus_tag="VFB89_14730"
     CDS             1776..2408
                     /locus_tag="VFB89_14730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZM28600.1"
                     /translation="MHKPRILIADDHAIFREGLALLLPPDMELVGAVSNGRELVEAAR
                     QLRPDVIVADLTMPGVGGLEALRILREEGLETKFVILTVHADPAIAARAIRAGAAGYV
                     VKDAAGNELLEALRFALAGRTYLTPLLSGDVVRRLAQGAPAPEDSLTPRQQEVLRLLA
                     EGRRVKQIAAELSLSVRTVETHKYEIMHLLQIENTVDLVKFAIRQGIVTV"
     gene            2492..>2767
                     /locus_tag="VFB89_14735"
     CDS             2492..>2767
                     /locus_tag="VFB89_14735"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZM28601.1"
                     /translation="MTLSGGWSPSSRARVLLADDNAMVAAEMQELLEPSLDVVGVVNS
                     GEALEAAFDLLSPDVVVADITMPGEGGLAAVEHIRERCPDTPVVLLTV"
BASE COUNT          430 a          869 c          924 g          544 t
ORIGIN      
        1 aggcggccct tagccccagg cggtccaaaa cctgtcattt ctgacagttt cgacgctgcc
       61 gcaagcaccg tatgctgtca aggagttcat ggacatgccg ggatccagcc cttagcccag
      121 gaggggctct gcttcgagta cgcgaggaag agccttgcta gctgccaacc cgtcggacgt
      181 cgacgattcc gtccccgatt ctaggttcct cccgggatct ggcttggccg gcgccagcgt
      241 ggtcgtcgct gccggtgtgg tggttttcct ggcccaatgg gtatcagtcg cgctctgggt
      301 accgccgatc cgggtgtcca gcgtatggct caccggtggc gtcatgctgg ccgcggccct
      361 tctcagccaa ccccgacact ggccggcggt gatcgcggca ggggcaaccg gccagacgtt
      421 tctgtttctg ggcatgcagc tggttagtcc tggcgcggcg attctgcttg gtctgctcgc
      481 tgcgctgcaa acggtggccg tggctctcgc actccgcgcc gtcgtccgtg gcagatatat
      541 cctgagcacc ctccgtgagt tccgggacta cggggtcgtc gccatcggcg gtggcgctat
      601 cagcgcttcg atcttgttcg tcgccggggc ctggagcatg cgattccggc cggcaacatt
      661 cttgacctgg cgcaccttcg ctctcgcagc ggttctaggc tatctgatgg tcacgcccac
      721 cttagtactg ctggtacaac ggatcatgca tccggctcga ggtgacccac gacgacggtt
      781 cgaggggagc ctcctgagcg tactgctgac ggtcgtctcc ggttcggtaa tcgtgctggc
      841 ggaacgcggc tcgttggcgt ggccggtgct ggtggccact ataccccctt tgctgctctg
      901 ggcggtcgtg cgcttcggcg cgctcggcgc cagtgcgtcg ctgttggtag tcgctctggt
      961 ttcgagcctg ggcgctgctc acgggcttac ggttttcgcc ggccgaaccc aggcagacca
     1021 catcctgttt ctgcagctct ttctgctcgg caccggagta ccgttgctcg gcatgggcgt
     1081 cgtcatggat gaacagaagc gcaccaccaa tgccctgcaa tcgacccgcg gccgtcttca
     1141 gaagctgaac cgtgatctca tgaaggcacg cgaagcggag gcgacccgca tcgcgcggga
     1201 gctgcacgac gacgtgggac agcgactcgc gctgatctcg atcggactca gccgcctccg
     1261 gcgctcggat gcgcagcgca cgggcgccga gatccggcgg cttcaggagc agacgggctc
     1321 agttgtcaga tcgctgcggg atttgtcaca tcaactgcat cccgcggtcc tggagcacgc
     1381 agggttgccc ttggcgctgc agatgaaatg cgaggaggtt ttccaggcca cgggaatcga
     1441 ggtacggctg gtcagccatg gcgaaacctc cgagatacca ccggacatcg ccctctgcct
     1501 cttccgcgtt gcccaggagg ccttgagcaa cgtcattcgg cactcggggg cacacctggt
     1561 ggacctatcc ctggaccggg agggcagtga gctgcggctc aaggtcaggg acgatgggcg
     1621 gggtttcact cccggtgccg cgcgtaacgg aaatgggctg ggtctctatc acgtcaccga
     1681 gcggctcggt gctttcggag gcagcttggc cctcgagagc gcaccgggag cggggaccac
     1741 gctccgggtc gcagttccgc tgaatggagt gcacgatgca taagccccgc attctcattg
     1801 ctgatgacca cgcgattttc cgagaggggc tcgcgctcct gctaccaccc gacatggaac
     1861 tcgtgggtgc cgtctccaac ggcagggagt tggtggaagc agctcgacag ctccggcccg
     1921 acgtcatcgt ggccgacctc acgatgcccg gagtgggcgg actcgaggcc ctcaggatcc
     1981 tcagggagga ggggctcgag accaagttcg tcatcctgac agtgcacgcg gatcccgcga
     2041 tcgcggcccg ggctatacgg gctggggccg ccggctacgt ggtcaaggac gcggcgggta
     2101 acgaactgct ggaggcccta cgctttgcac tggcgggccg gacctacctt accccgttgc
     2161 tgtccggaga cgtggtccgc cggctggccc agggcgcgcc tgctcccgag gactccctaa
     2221 cgccgcggca gcaggaggtg ctacggcttc tggcagaagg ccgccgggtc aagcagattg
     2281 cggccgagct gagcctgtca gttcggacgg tcgagaccca taagtacgag atcatgcacc
     2341 tgctccagat cgaaaacacc gtagatctgg tcaagtttgc catccggcag ggcatcgtga
     2401 cggtgtgacc ccctgattcc gcagattttc gtaccccgcc gcacgtagtt gttccgaggc
     2461 gaagcatcgg gtaaccggct agggttggtc tgtgacctta tctggcgggt ggagcccgtc
     2521 gtcgcgggct cgcgtccttc tcgccgacga caacgctatg gtcgcggccg agatgcagga
     2581 gctgctcgaa ccgtccctgg atgtcgtcgg ggtggtcaac agcggcgagg cgctggaggc
     2641 tgccttcgat ctgttgagtc cggacgtcgt cgtggcggat atcaccatgc cgggtgaggg
     2701 cgggctggcc gcggtcgagc acattcggga gcggtgtccc gatactccgg tggttctcct
     2761 caccgtt
//
LOCUS       DASRJQ010000652         5023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1006361, whole genome shotgun sequence.
ACCESSION   DASRJQ010000652 DASRJQ010000000
VERSION     DASRJQ010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006361"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="VFB89_14740"
     CDS             complement(<1..778)
                     /locus_tag="VFB89_14740"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HZM28602.1"
                     /translation="MHFTCAARTDVGVVRSGNEDNYLMLSDRGIFIVADGMGGHAAGE
                     VASEMAVRIASREIGSIRGLTEPQVGERIRAAIRMANDAIFERTLAEHDKRGMGTTAT
                     VLVLMRGRYLVGQVGDSRAYLLRDGQFSQLTKDHSYVQEQVDAGLLTPEQARVHPYSN
                     VITRCVGASVDVVPDLYFGVLKPGDIVLLASDGLTGMLEDELLVKILQSEGDPQTWVD
                     KMIAEANRRGGLDNITAIVVRIDAVDQPTGETPAVTGATGA"
     gene            complement(789..2105)
                     /locus_tag="VFB89_14745"
     CDS             complement(789..2105)
                     /locus_tag="VFB89_14745"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZM28603.1"
                     /translation="MMQLEVGGLRYPIAAGEMVVGSDPSCTLTLNGAGVHPRHAIVQA
                     LPDGSAAIRRASPDAEVLVNGVSRGDDPTPILHGDKIRIGPHELLVVDPARAGHTKFF
                     DSSAFEKFTPSGGSKPAAPTVVAGGRLVCLTDGREYQVGAALVIGRDASADVVVPGNE
                     VSRRHAQIVATEQGFVLVDTSTNGTFVNGHRVEGSRVLRRADVIRVGADEFRFYGEPS
                     PSTTTSDPAAPATAEPESGAEPPPAGAQQKLHDTMFGVGPPPGPVMKGPVTPITPMDT
                     PIASLLVRTGSLKGKRLTVRTPVVNIGRGDYNDLVIPEPSVSATHAKLQRREGIWIIQ
                     DLGSTNGTFVDGERVAEETPLGPGATIRFGEVATLFESTDDTTGVQVRVGTKMVEGLR
                     TPPEREATPPRRPARVAAPEPKGFPRWAIVVAALVVAGVIAFMLLR"
     gene            complement(2158..3981)
                     /locus_tag="VFB89_14750"
     CDS             complement(2158..3981)
                     /locus_tag="VFB89_14750"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZM28604.1"
                     /translation="MTAPIETEEPLNRGYDAGLLKRLLIYLRPYKAPVFAAVLLLLGG
                     AALTLVGPALTQRALDIAIPRRDAALLGTLAALYLGALLLEFLFEYGQTLLTTYVGQR
                     IMYDLRLQIFSHLQRLSIPYFDRHPVGRLMTRVTSDVETLNELFSSGVVTVFGDVFTL
                     AAIMVMMLVTDWRLALVAFAVIPLVWLTVTLFRRSVRSAFRDIRIRLARLNAYLQEHL
                     SGVRVVQLFGREQASSAEFRRINADHLEAHLRSITIYAIFFPVVEALSSIALALLLWY
                     GGLRTLGGTLTVGVLAAFIQLTRRFFQPLQDLSEKFNLLQSAMASSERVFRLIDEPVT
                     IPEPSSPRPLARPIRGEVVFDGVWFRYAPDGPWVLKNVSFTARPGEMIALVGHTGAGK
                     TTVVSLLLRFYDAERGRITIDGVDIRDLSLSDLRGLIGFVQQDLFLFTGDILHNLTLD
                     APVAPSAAEQAARRVGASRFVERLPQGYGHVLGERGRNLSVGERQLLSFARALARDPH
                     ILVLDEATSSVDAEAEAQIQRAIEELMAGRTSVVVAHRLSTILHADEILVMHHGEIRE
                     RGSHRELLAQRGVYERLYQLQLRGQLPAEPVNAVTHAGLRG"
     gene            complement(3978..>5023)
                     /locus_tag="VFB89_14755"
     CDS             complement(3978..>5023)
                     /locus_tag="VFB89_14755"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HZM28605.1"
                     /translation="RGFEFFLIASLLGYGLTFLYGKDVSASIAKFAQLDWRWLIVGIG
                     LASMDWFGGGLRLWVLARHVVPRPSLKGCILAGGMGAWAAYVTPLNSGAGPMTMYTLR
                     RYGIPLPVAVTSTLMSFIATVAFFGIAGPLAISLGAGHSLGTKGNVLGLSLYDLFLGS
                     LGVVGLIGLVLIAIIVFPRFAANLIHRLAVFVGRRSQRVADRLEHVTKGIEEAHASVV
                     AFNSLRGWIALGWATLLSGPSHANKLLAGYVALRTLGIHVNFVDVLLVQTLVMFLLYF
                     APTPGASGIAELLSAAVMSVYVPRGITPLYTLVWRFILSYCTLGVGFVVFSGWVRKGL
                     KAVDETALPEAAT"
BASE COUNT          968 a         1641 c         1600 g          814 t
ORIGIN      
        1 ttgcccccgt cgcccccgta accgccggcg tctctcccgt cggctggtcc accgcgtcga
       61 tccgcaccac gatggcagtg atgttgtcga gcccgccgcg acggttggcc tctgcgatca
      121 tcttatcaac ccaggtctgt ggatctcctt ccgactgcag gatcttgacc agcagctcgt
      181 cctccagcat gccggtgagc ccgtcggacg cgagtaacac gatgtcgccc ggcttgagca
      241 ctccgaaata gagatcgggc accacatcca cgcttgctcc gacgcagcgg gtaatgacgt
      301 tgctgtaggg gtggacgcgg gcctgttccg gggtgagcag gcccgcgtcg acctgctcct
      361 gcacgtagga atggtctttg gtgagctggg agaactgccc atcgcggagc aggtaggccc
      421 ggctgtcccc tacctggccg accagatagc gaccgcgcat caggaccagc acggtcgcgg
      481 tggtacccat tccacgtttg tcatgctccg ccagcgtgcg ctcgaagatc gcgtcgttgg
      541 ccatccggat ggcggcccga atccgctcgc cgacctgcgg ctcggtcagg ccgcggatcg
      601 agccgatctc gcgcgaggcg atgcgaacgg ccatctcgct cgcgacttcg ccggcagcgt
      661 ggccgcccat tccgtccgcg acgatgaaaa tgccgcggtc ggagagcatg aggtagttgt
      721 cttcgttgcc cgaccggacg acgcctacgt cggtgcgggc cgcacaagtg aaatgcacgg
      781 agctccgttt atctcaacag catgaacgcg attaccccag cgacgacgag cgccgcgacc
      841 acgatggccc agcggggaaa gcccttgggc tcgggtgcgg cgacgcgcgc ggggcgccgc
      901 gggggggtgg cttccctctc gggcggcgtg cgaagtccct cgaccatctt ggttccgact
      961 cgcacctgaa cgccggtcgt gtcgtccgta gattcgaaga gcgtcgccac ttcgccaaac
     1021 cggatcgtcg cgcccgggcc gagcggggtc tcttccgcta ctcgctcacc gtccacgaac
     1081 gtcccgttgg tggagccgag gtcctggatg atccagatgc cttcccgccg ctgcagcttc
     1141 gcatgtgttg cgcttacgct cggctccggg atcaccagat cgttgtagtc gccccgaccg
     1201 atattgacca ccggtgtccg aaccgtaagg cgcttgcctt tgaggctgcc cgtacgcacc
     1261 aaaagcgacg cgatcggcgt gtccatcggt gtgatcgggg tgaccggtcc cttcatgacc
     1321 gggccaggcg gcggtccgac gccgaacatc gtgtcgtgca acttctgctg cgcccccgcg
     1381 ggcggcggct cagcgccgct ttccggctcg gcagtggccg gggccgctgg atcggaagtc
     1441 gtcgtggagg ggctcggctc gccataaaag cggaactcgt cagcgccaac ccggatgacg
     1501 tccgcgcggc gcagcactcg agacccctcg actcgatgac cgttcacgaa cgtgccgttg
     1561 gtactggtat ccaccagcac gaacccttgc tccgtcgcta cgatctgggc gtgccgacgt
     1621 gacacttcgt ttcccggtac cacgacatcc gcgctggcgt cgcgaccgat gacgagcgct
     1681 gcgccgacct gatactcccg gccgtcggtg aggcaaacca gccggcctcc ggccacgacg
     1741 gtcggggcgg cgggcttcga tccaccggaa ggggtgaatt tctcgaaggc tgacgagtcg
     1801 aaaaacttgg tgtgaccggc gcgggcagga tcgacgacca gcagctcgtg aggtccgatc
     1861 cgaatcttgt cgccgtgaag tattggagtc ggatcgtctc cccgggacac gccgttcacc
     1921 agaacctcgg cgtcaggcga ggcccggcgg atcgccgccg atccatcggg gagcgcttga
     1981 acgatagcat ggcgcggatg aacgcccgcg ccattgagtg tcagggtgca gcttgggtcg
     2041 gacccgacga ccatttcccc ggctgcgata gggtatcgca agccgccaac ttcgagctgc
     2101 atcatggaag cgggtccggg cgtgtcatcg agtgccagtc aacctatccg tggggtccta
     2161 accgcgcaag ccagcgtggg ttaccgcatt gaccggctcc gccggaagtt gtcctcgcaa
     2221 ctgcagctga tacagccgct cgtagacacc gcgctgagcc agcaactcgc gatggctgcc
     2281 tcgttcacgg atctcgccgt gatgcatcac cagaatttcg tccgcgtgaa ggatggtgct
     2341 gagtcggtgg gccacgacga cgctggtccg tcccgccatc agctcttcga tcgctcgctg
     2401 gatttgggcc tccgcttccg catccaccga actggtggct tcgtccagca ccagaatgtg
     2461 cggatcccgc gccagtgcac gggcaaagct caggagctgc cgttcaccca cgctgagatt
     2521 ccggccgcgc tctcccagca cgtgaccata cccctgcggt aagcgctcga cgaatcggct
     2581 tgcgcctacc cgacgcgctg cctgctccgc cgctgaggga gcgaccgggg cgtcgagtgt
     2641 caggttgtga aggatgtccc cggtgaagag aaacaggtcc tgttgcacga acccgatgag
     2701 gccccgcaga tcgctcagcg agagatcgcg aatgtcgacc ccgtcgatgg tgatcctgcc
     2761 ccgctcggca tcgtagaatc gcagcagcag actgacgacg gtcgtcttcc ctgccccggt
     2821 gtgacccacc agcgcgatca tctccccagg tcgagcggtg aacgagacgt tcttgagaac
     2881 ccagggtccg tccggggcat agcggaacca gacgccgtcg aacacgacct ccccccggat
     2941 gggcctggcc agcggccgag gagagcttgg ctccggaatg gtgaccggct cgtcgatcaa
     3001 ccggaacacc cgttccgagg aagccatcgc gctctggagc agattgaact tttccgacag
     3061 gtcctgcaac ggctgaaaga agcgccgggt gagctgaatg aatgccgcca gcacaccgac
     3121 cgtgagggtc ccgccgaggg tgcgcaatcc accgtaccag agcaacagcg ccagcgcgat
     3181 cgagctcagc gcctccacga ccgggaagaa gatcgcgtag atcgtgatcg agcggagatg
     3241 ggcttcgagg tgatccgcat tgatgcggcg gaactcggcc gacgacgcct gttcccggcc
     3301 gaacagctgc accacgcgta ctcccgagag atgctcctgc aagtatgcgt tcagccgagc
     3361 cagacgaatc cggatgtcac ggaacgcgct ccgcacgctc cggcggaaca gtgtcaccgt
     3421 gagccatacc agcggaatga ccgcgaacgc caccagggca aggcgccagt cggtcaccag
     3481 catcatgacc atgatcgcgg cgagcgtgaa cacgtcgccg aacacggtca cgacgccgga
     3541 ggagaacaac tcgttcagcg tctccacgtc ggaggtaacc cgggtcatca gacggccgac
     3601 cggatgccgg tcgaagtacg gaatgctgag tcgctggaga tggctgaaaa tctggagccg
     3661 caggtcgtac atgatgcgct ggccgacata cgtggtcagc agggtctgac catactcgaa
     3721 caggaattcg agcaggaggg caccgagata gagcgcggcg agagtaccca gcagcgcggc
     3781 gtcgcgccgg ggaatggcga tatcgagggc tcgttgtgtc agggcaggcc cgaccagcgt
     3841 gagggcagcg cctcccaaca gcagcaggac agccgcaaag accggagcct tgtacggtcg
     3901 caggtagatc agcaggcgct tcagaagacc ggcgtcgtac ccgcgattga ggggttcctc
     3961 ggtttcgatc ggggcggtca tgtcgcggcc tccgggaggg ccgtctcatc cactgctttg
     4021 agaccctttc ggacccagcc ggagaacacc acgaaaccga cgcccagtgt gcagtagctc
     4081 agaatgaacc gccaaaccag agtgtagagc ggagtgatcc cccgcggaac atagacggac
     4141 ataaccgccg cgctcagcag ctcggcgatg cccgacgcgc ccggcgtcgg agcgaaatac
     4201 aggaggaaca tcaccagagt ttgaaccagc agcacgtcca cgaagttcac atgaatcccc
     4261 agtgtacgga gcgcaacgta ccccgccagc agcttgttgg catgcgaagg cccggagagc
     4321 aatgtggccc agccgagcgc gatccagccg cgcaggctgt tgaacgcaac aacgctggca
     4381 tgcgcttcct cgatgccttt ggtcacgtgc tccaatcgat cggccactct ctggctgcgc
     4441 cgaccgacga acacggccag tcgatgaatc aggtttgcag cgaaccgcgg gaacacgatg
     4501 atcgctatca gcaccagccc gatgagcccc accacgccca ggctgccgag gaacagatcg
     4561 tacagcgaga gtcccagcac gttgcccttg gtgcccagcg agtgaccggc accgagcgag
     4621 attgccagcg gacctgcgat cccgaagaag gcgactgtgg cgatgaagct catgagggtc
     4681 gacgtgacag cgaccggaag aggaattccg tagcgccgca gggtatacat cgtcatcggc
     4741 ccggcacctg agttgagcgg cgttacgtac gccgcccagg cacccatccc gccggcgaga
     4801 atgcagccct tgagcgaagg ccggggaacg acatgtcgcg ccaggaccca gaggcggagc
     4861 cctccgccga accagtccat cgatgcgagt ccgattccca cgatgagcca gcgccaatcc
     4921 agctgcgcaa acttggcgat cgaggcagag acgtccttgc cgtacaggaa tgtgagaccg
     4981 tacccaagca gggatgcaat gagaaagaac tcgaagcccc gga
//
LOCUS       DASRJQ010000653         2879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1240398, whole genome shotgun sequence.
ACCESSION   DASRJQ010000653 DASRJQ010000000
VERSION     DASRJQ010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240398"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VFB89_14760"
     CDS             <1..666
                     /locus_tag="VFB89_14760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28606.1"
                     /translation="HRVPEHVLQRLIGRWFYASTLSGRYTGSPETVMDGDLNRVRDLP
                     SADAYVGALERIIASTLTPDFWTITLPNALDSSSARNPELFAYHASQNKLGAPVLFSH
                     KRIADLLDPALKTKKRATERHHLFPRAWLERQGITDLKLINQTANMAFLEWPENLEIS
                     DKPPQEYVPLMRARFSDEDWKRMCQMHALPDGWETMGYEEFLAARRPLMAQIIKRGLE
                     MLR"
     gene            666..848
                     /locus_tag="VFB89_14765"
     CDS             666..848
                     /locus_tag="VFB89_14765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28607.1"
                     /translation="MGASELRHRCVESLVPGRLRRSRDFGGRVAEEDWQFTKTYARIA
                     GRWQVVAFHASPTPSP"
     gene            882..1352
                     /locus_tag="VFB89_14770"
     CDS             882..1352
                     /locus_tag="VFB89_14770"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZM28608.1"
                     /translation="MKPKLYVETSIVSYLTALPSRDLVRAAHQQVTRDWWTTRAQFDL
                     YVSQFVIDEASAGDERAAADRLAALREATLLNTTPDAVSLARELIRMGDLPAKAVVDA
                     FHIAIAAVHGMDYLLSWNCRHIANAMMRGRIEATCRSLGVEPPTICTPIELAKE"
     gene            1358..1540
                     /locus_tag="VFB89_14775"
     CDS             1358..1540
                     /locus_tag="VFB89_14775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28609.1"
                     /translation="MPSADPILEEIHAIREALAKKAGYDAEKIAEAARRRQAESGRKA
                     VTLPPRPAATVKKKAS"
     gene            1604..2551
                     /gene="glyQ"
                     /locus_tag="VFB89_14780"
     CDS             1604..2551
                     /gene="glyQ"
                     /locus_tag="VFB89_14780"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HZM28610.1"
                     /translation="MGISMRKTFQELILKLSEFWAREGCVLQQPLDLEVGAGTMHPDT
                     FLRVLGPEPWRVAYVQPSRRPADGRYGDNPFRLGKHHQFQVILKPSPHDVQQLYVRSL
                     EAIGLDLTNHDLRFEEDNWEAPTLGAWGVGWQVMLDGMEITQFTYFQQAGGLELKPIS
                     AEITYGLERLTMFLSLSRNIYDIEWVEGFLYGQVRHQEEVELSKYYFEVADVTFLQQQ
                     FEGYEREAGRCLEAGLVLPAYECALKCSHSFNVLDARGAVSVTERVALMKRVRELAVG
                     CAKAYVESRERLGFPLLEREIPFPPPPPPTFEEIVDAAR"
     gene            2538..>2879
                     /locus_tag="VFB89_14785"
     CDS             2538..>2879
                     /locus_tag="VFB89_14785"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HZM28611.1"
                     /translation="MPQGEYLLEVRSEEIPARMLEPGVRELATRVFEELMARGLGPRE
                     VETGYSPRRLVLILSGVPEREPDREEQVLGPPVSAAFAPDGSPKPALTGFAKRTGVEP
                     GELSRVKTDKGE"
BASE COUNT          516 a          954 c          927 g          482 t
ORIGIN      
        1 caccgggtgc cggagcacgt tctgcaacgg ttgatcggtc gctggttcta tgccagtacg
       61 ctgtcggggc gctacacggg ttcgccggaa actgtcatgg atggtgacct caaccgtgtg
      121 cgcgatctgc cgagcgccga cgcctacgtg ggtgcgctgg aacgaatcat tgcgagcact
      181 ctgactcccg acttctggac gatcaccttg cccaatgccc tcgacagctc gtcggcgcgg
      241 aaccccgagc tcttcgccta tcacgcctca caaaacaaac tgggcgcacc ggtgttgttc
      301 tctcacaaac ggatcgcgga tctcctcgat ccggcgctca aaaccaagaa gcgggcgacc
      361 gaacgccacc acctgtttcc tcgggcatgg ctcgaaaggc aaggcattac ggatctcaag
      421 ctcatcaacc agaccgccaa catggccttt cttgagtggc ctgagaacct ggagatcagc
      481 gacaagcccc cccaggagta cgtgccactg atgcgagccc gattctcaga cgaagactgg
      541 aagaggatgt gccaaatgca cgctcttccg gacggttggg aaacgatggg ctacgaagag
      601 ttcctcgccg cgcgaaggcc gttgatggcg cagatcatca agcgtggctt ggagatgctc
      661 cgataatggg cgcatcggag ttgcgccatc gatgtgtaga atcgctggtc ccggggcgct
      721 tgcggcggag ccgcgacttc ggcggccggg tcgccgagga ggattggcag ttcaccaaga
      781 cctatgcccg catcgccggc cgctggcagg tggtggcgtt ccacgcctcg cccacgcctt
      841 cgccttgata cccgaccact gaatgagggc aaaccgccac gatgaaacca aaactgtatg
      901 tggagacgag catcgtcagc tatctgactg ccctccccag ccgagacctg gtgcgcgcgg
      961 cgcatcagca ggtcacccgt gactggtgga ccacacgggc gcaattcgat ctgtatgtct
     1021 ctcagttcgt catcgacgaa gcatccgccg gggatgagcg tgcagcggcc gaccggcttg
     1081 cggctctccg ggaggcaact ctgctcaaca cgaccccgga tgcagtctcg ctggcgagag
     1141 aactgatccg catgggcgat cttccggcaa aggcggtcgt cgatgcattc cacatcgcca
     1201 tcgctgccgt ccatggcatg gactatctcc tgagctggaa ctgcaggcac atcgccaacg
     1261 caatgatgcg tggtaggatt gaggccacat gccgatcgct cggtgtcgag ccgccgacga
     1321 tctgcactcc gatcgagctc gccaaggaat gacagagatg ccgtcagccg accccatcct
     1381 cgaagagatc cacgcgatcc gcgaagcgct cgccaagaag gctggctatg atgccgagaa
     1441 gatcgccgag gcggctcgca ggcggcaggc cgagagcggg cgaaaggcgg tcacgctgcc
     1501 gccccggccc gcagctaccg tgaagaagaa ggcttcctga cggcctgccc cccgcccttc
     1561 cggatcggat agactaccgc ctctgccggg ggcctctgcc cccatgggaa tctctatgcg
     1621 aaagaccttt caggagctca tcctcaagct gtccgagttc tgggcgcggg aggggtgcgt
     1681 cctccagcag cccctggacc tcgaggtcgg ggccggcacc atgcacccgg atacgttcct
     1741 gcgcgtgctc ggtcccgagc cctggcgggt ggcgtacgtc cagccctccc gccgtccggc
     1801 cgacggccgc tatggcgaca atccgttccg cctgggcaag caccaccagt tccaggtgat
     1861 tctcaaaccc tcgccgcacg acgtgcagca gctctacgtc cgcagcctgg aggcgatcgg
     1921 gctcgacctg acgaatcacg acctgcgctt cgaagaggac aactgggagg ctcccacgct
     1981 cggcgcctgg ggcgtgggct ggcaggtcat gctcgacggc atggagatca cccagttcac
     2041 ctacttccag caggcgggcg ggctggagct gaaacccatc agcgccgaga tcacctacgg
     2101 cctggagcgg ctcaccatgt tcctctccct gtcgcggaac atctatgaca tcgagtgggt
     2161 cgaggggttc ctctacgggc aggtccgcca ccaggaggag gtcgagctgt cgaagtacta
     2221 cttcgaggtg gcggatgtga ccttcctcca gcagcagttc gagggctacg agcgcgaggc
     2281 cggacgctgc ctggaggccg ggctcgtcct gcccgcctac gaatgcgctc tcaagtgctc
     2341 gcactcgttc aacgtcctcg acgcccgcgg cgcggtgtcg gtcaccgagc gcgtcgctct
     2401 catgaagcgg gtgcgcgagc tggccgtggg gtgtgccaag gcctacgtcg agagccgcga
     2461 gaggctgggc ttcccgctgc tcgagcggga gatcccgttc ccgccgccgc cgccgccgac
     2521 cttcgaggag atcgttgatg ccgcaaggtg agtacctgtt ggaggtccgc agcgaggaga
     2581 tcccggcccg catgctggag ccgggcgtgc gcgagctcgc cacccgcgtg ttcgaggagc
     2641 tgatggcgcg cggcctgggt ccgcgcgagg tcgagacggg ctactcgccg cgccggctcg
     2701 tgctgatcct gagcggcgtg ccggagcgcg agcccgaccg cgaggagcag gtgctgggtc
     2761 cgccggtcag cgccgctttc gctccggacg gctcgcccaa gcccgcgctc accggcttcg
     2821 ccaagcgcac cggcgtcgag cccggcgagc tctcccgcgt caagaccgac aagggggag
//
LOCUS       DASRJQ010000654        14727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2038404, whole genome shotgun sequence.
ACCESSION   DASRJQ010000654 DASRJQ010000000
VERSION     DASRJQ010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 14727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14727
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2038404"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..729)
                     /gene="hisB"
                     /locus_tag="VFB89_14790"
     CDS             complement(139..729)
                     /gene="hisB"
                     /locus_tag="VFB89_14790"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF002111.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HZM28612.1"
                     /translation="MSRIGEVRRKTKETDLHVRLVLDGTGAAQVRSGIGFFDHMLEAF
                     ARHALIDLTIEGSGDLHVDGHHTVEDTGIAIGQAIAQALGDRAGVRRYADALVPLDEA
                     LVRAVVDVSGRPYLSYHVDIPKWQMLGDYDVFLTPEFFRALVLNAGLTAHIDLVRGDN
                     PHHIVEATFKAFARAMDAATTLDPRVTGVPSTKGVL"
     gene            complement(754..1851)
                     /gene="hisC"
                     /locus_tag="VFB89_14795"
     CDS             complement(754..1851)
                     /gene="hisC"
                     /locus_tag="VFB89_14795"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HZM28613.1"
                     /translation="MKQPLADPGPALALIKPSVRGQPAYALKPFEARRKLNQNESPYD
                     VPPELKREILERMAGASWHRYPEFVPARLLAQLSTRFNWPTDGILVGNGSNELIQATL
                     AVSLETGDRVVAPTPTFSLYRLLTNVFGGCYVPVAFGADFRYNVEALSEVATAERARV
                     IVLNSPNNPTGSALPPDGVDRVLEDTDALVLCDEAYQDFGGTTALGLLASSPRVVVLR
                     TFSKALGLAGLRLGVALAHPSVAREIAKAKLPYNVNVLSLTAAEVLLGHPDLAGAHVP
                     EIIATRDRTIQQLNSTPGLRVFPSAANFILIRCVALPAAEVFRRLLEEHGILVRDVSG
                     APELAECLRISVGTPEDMEAVVAGLGQILKA"
     gene            complement(1848..4085)
                     /locus_tag="VFB89_14800"
     CDS             complement(1848..4085)
                     /locus_tag="VFB89_14800"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HZM28614.1"
                     /translation="MQFTDEWLVPTLETLVPEDALKALRGTEGAPASVWEAVVQRKLA
                     TDAQILGAIASRFRLPVADFSGLDPRVKDEIPEQLVRKFNVLPLKVTDAYLEVATANP
                     FDLDAEKTLAFATGREVRMALGSPARIREKLDDLYRNDDVVSRLLEGIGGDFDVKEVA
                     DEDDAAASAEQASQRPIVRLVDMMLADAATSRASDIHVEPAEGGVVIRYRIDGVLRQV
                     MRIPRTAGIPLISRVKIMSGLDIADRLRPQDGRARITVNNEPIDLRVSTLPASMGEKV
                     VIRLLSSKGTILALDGLGLFEDEADQIRRLLHFKEGILLVTGPTGSGKTTTLYSAIRV
                     IQGEGVNIVTVEDPVEYRLGANIVQVQVHEKANLTFASALRSILRQDPDVVLIGEIRD
                     QETAQIAVQASLTGHLVLSTLHTNDAPNAVTRLLDMGMEAYKIGSALRGVVAQRLMRR
                     LCPICKEVSSDPVPERLAQFIPAGTVLYRAVGCPECASTGYRGRFSIVEVLAMNPELE
                     RLIGSGGTADRIAEMGRRNGMKSLWDSGLRHMLRGETTLDELLRVTDVPQTSVREPVS
                     PPPPPRSGRRGATAPPPAPPPPVAAPQAPPAFDFGLDLELLDESPPADGYAQGGRGIC
                     VLLVDDEDDLRRVLRDLLEREGFTVQEARDGIQALDEVDRHAPDIIVLDLNLPGLDGY
                     SVLAQLRSRQVTKDIPIVVLTAKGDEENEVKVFELGADDFLSKPFRARALAARLEAVL
                     GRRGR"
     gene            complement(4183..6264)
                     /locus_tag="VFB89_14805"
     CDS             complement(4183..6264)
                     /locus_tag="VFB89_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28615.1"
                     /translation="MRGNPASMTVTAPRAVALVLSLLLVRCSSLTAQEGWNSAAALTL
                     ARRGVERREAVQADPTLQSYRTRAHGFVFFLTQIGKEGLGGPPRLVKADELDVEVYWQ
                     APNRSKQVIHGWRDGSFLPTDIAYHRDHLGIVTNNFGDLIRIGEGDEVRDAVHPLSPV
                     GLDAYDFSLGDSLTIRGPRGDVKVRELLIRPRQYDRPLVVGSLYLDAESAELVRFRFS
                     FTPAAYLERNLEDISVVLENSLQEGRFWLPYRQEIEIRRRASWFDFPVRGIIRGRWEI
                     GDYEFNVPLPPSVLEGPEIGGLTEPQPTDSAWPTSLRDAIAAEAAPIDRQDMEALRVD
                     ISRLASTQRLSGLPPTRLAVGSVSDLVHVNRVQGLTLGLGGIAHFGGGRFGVRPTIAF
                     GTSDQRVTGGIELTAGLGATELGIFGQRRIRDLSDIPVISGALNSVLAQENGDDYGDY
                     VLLDAAGARLRHRLGSRLGLGFEAGVEHSTSLDVEATPARGSYRPNPPLGAGTYGVLR
                     ARLERAGGGLADAADVTGSLAVEAGEGHTSYARVLLDASWRAPLGRSALAGKLQVGMG
                     TDSLPAYRGFVLGGRGTLVGEPFRGFGGRSYALAELEWRVDVPAPAFPLGSFASTGHT
                     MVLAPFFAAGWSERPYDGLPWPATDGVRPVAGVALEFFLRLLRVEAGVGLRDGDFGLT
                     FDVHPDWWGVL"
     gene            6242..6877
                     /locus_tag="VFB89_14810"
     CDS             6242..6877
                     /locus_tag="VFB89_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28616.1"
                     /translation="MEAGFPLICPRASRRGFRIFRGLAAGTIALTAACAAPRPAITVK
                     PTDTTTGDPSRTYSLSQVILLHVDSLSPDDTVVKVRRGQPRVIVIRHPQPDELAFADL
                     ALPAQAFDSAAGDSVTVSVHIRPGLYGLDLTTSAPLKQGTLTFKYPVHFAAPAEATAA
                     FGSDVAFEHVLAIGKLGPNETIVFQRSSRPASDNLSVALTSAGSYVVGAPK"
     gene            6874..8382
                     /locus_tag="VFB89_14815"
     CDS             6874..8382
                     /locus_tag="VFB89_14815"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HZM28617.1"
                     /translation="MIDRFASFAERARRAAGITPITREVVLDGDTAVTAFSKIHRGPY
                     GFLLESLEGGERWARYTFLATDPREVFRYRGRVCERWTSTTGWQIADPDVAPLDHLGR
                     TMRQHAPVEVPGLPRFTGGAVGYLGYDIVRTIESLPSPPKDDRGLPDAILMVADTLLV
                     LDNVYNRATVIANVEVPASAAEPDLRRLFEQGEARIDDWLCRLQAPVTAGPLPLDLPD
                     SSPQATSPYADHDFQDDVRRIKEYIAAGDTFQTVLSRRLDLSAPDPFLTYRYLRALNP
                     APYLYYLQCDDMYVVGSSPEILVRVEDGEVILRPIAGTRPRGETPDRDAALAAELEAD
                     PKERAEHLMLVDLGRNDVGRVAEFGSVRLTDFMTIERYSHVMHLVSEVRGKLRPGLDA
                     LAALAACFPAGTVSGAPKVRAMEIIDELEPVRRGPYAGAVGYVGWGAQVLDTAIALRT
                     CVIQGGRAWVQAGAGIVADSDPAAEWRETEAKARAVMLALALASRAQMPRYP"
     gene            8393..9949
                     /locus_tag="VFB89_14820"
     CDS             8393..9949
                     /locus_tag="VFB89_14820"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HZM28618.1"
                     /translation="MFSYRLVSLSGNQRHDLPAGRTMVVGRGVGCDITVYDPTISRRH
                     AELRAGPDGVSLKDLGSSNGTYINGRRATNARLSVNDTVTFGKVGYQLEAVPVEPERP
                     SASATGASTIVREVAAAGPTSGDVSARKLSLLLSMAQKLTGEFDIDRLLDTIAAVSFE
                     VMRVDRVAILLRQDNTGELITRLSRARLGGAEVERVSGSIVRKAMDEQVAVLTQNAAT
                     DPRFKGQSIRMQNVRSAMCSPLMASADEALGVLYVDSVTAENAFTDEDLQFLIAFSGL
                     AAVAIQNSRYAEQARRETLVRSNFERYFAPDVAADIIRQKTPIQPGGDRRAMTILFAD
                     IRGFTAIAEGMSPDAIAEMLTEYFSEMVDVIFEHGGVLDKFIGDALMALWGSPLAREH
                     DPRRALEAAHAMQRAVDALNGRWHAAGRPEIGVGIGINYGEVFVGNIGSLRRLEYTVI
                     GDAVNVAQRLSSEAAAGEILVSEAYRRVSGDVVDAEEPAGLGLRGKSQAVEVFRVRRE
                     RASADGGGRE"
     gene            complement(9994..10590)
                     /locus_tag="VFB89_14825"
     CDS             complement(9994..10590)
                     /locus_tag="VFB89_14825"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HZM28619.1"
                     /translation="MSGPTLVLASGSPRRRQLLEMLGLEVEVRPPHIPEIRGADELPQ
                     PYVERLAREKAASVSGDLVLGADTTVVVEGEMLEKPSDAADALRMLRRLQGRTHHVIT
                     AIALKTPAGTLGATDVTAVSFRPADDELLAAYVATGEPMDKAGAYGIQGFGAALVEGI
                     QGDFFGVMGLPLRLVLQLLERAGHPYFFGQERAGTGPA"
     gene            complement(10587..11030)
                     /gene="dtd"
                     /locus_tag="VFB89_14830"
     CDS             complement(10587..11030)
                     /gene="dtd"
                     /locus_tag="VFB89_14830"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR00256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HZM28620.1"
                     /translation="MRVVLQRVSRAAVKIDGRVVGSIGAGFCLLVGFTHSDTTAQVEW
                     MAEKISGLRLFSDAEGKMNLGLAEVDGKVLVVSQFTLYSDAEKGRRPSFIDAARPETA
                     IPLYESFVAALRERKLEVATGRFGAMMEVELVNDGPVTLVLERST"
     gene            11126..12004
                     /locus_tag="VFB89_14835"
     CDS             11126..12004
                     /locus_tag="VFB89_14835"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZM28621.1"
                     /translation="MAQLYKTDEIDPAGSEQRSCRVRPAPRRPHPDRWPDGQATFAWL
                     GHATVLANFFGVWLLTDPALEARIGVGRGRAKLGPRRLTAPALRPAELPQLDLLLLSH
                     AHMDHTDLGTLRSLRRDVPVVVQPGNSDLVRRFRKVDELPWGERVTVAGIEIESVPVR
                     HWGARMITDRHRGYGGYLLRRSGRTVLFAGDTAYTDAFAPLGRRGPIDLAILPIGAYD
                     PWIANHASPEEAWRMARQMNATHVLPVHHSTFRLSREPMHEPIERLLNAAGPERDRIV
                     IREIGQTWTLPEGPAD"
     gene            complement(12111..13940)
                     /gene="glmS"
                     /locus_tag="VFB89_14840"
     CDS             complement(12111..13940)
                     /gene="glmS"
                     /locus_tag="VFB89_14840"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HZM28622.1"
                     /translation="MCGIVGYIGPRPAAGLLLDGLKRLEYRGYDSSGIAIVNGKGLEI
                     AKAAGKISVLEQQLEGELPAGTVGIAHTRWATHGAPTTPNAHPHTDQSGRIAVIHNGI
                     IENAAAIRKALQQRGHTFKSETDTEVLAHLVGEFYRGNLEEAVAAALRDVDGAYGLAF
                     VSADEPGVLVAARKGSPLLVGVGENEWFVASDASPLLQHTRSVIYLDDGEMAILSRDG
                     YRIRNLETAQIEKPVNQIEWDLAAIERGGYSHFMLKEICEQPESLRNTLRGHLLEDEG
                     TSRVTGLNLSDDDLKQIQRIIITACGTSWHSGLIGEYMLEEMARVPVEVEYASEFRYR
                     NPVVDNKTLVVGISQSGETADTLAAIREAKRRGARTIGVVNVVGSTIAREVDGGLYLH
                     AGPEVGVASTKAFTSQVAALALISLRIARLRNLSILQGRQIIQALGKLPDQMANILER
                     ASEVEQLADRFLSTGHNALYLGRGVNFPVALEGALKLKEISYIHAEGYPAAEMKHGPI
                     ALIDENMPVVFIAPKDAVHSKIVSNIEEVKARGGKVIALVNPGDAEIERLADATFTIP
                     ETLDLLTPMLTSLPLQLLSYYVAVRRGCNVDQPRNLAKSVTVE"
     gene            complement(13955..>14727)
                     /locus_tag="VFB89_14845"
     CDS             complement(13955..>14727)
                     /locus_tag="VFB89_14845"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28623.1"
                     /translation="GVRVSGIHLDPDGRFPHPPEPIPENLRDLGNLVRESGADLGLAV
                     DPDVDRLALVDEQGNAIGEDYTLALAVRAVLAGTGNPNGGPPVVVVNLSTSLVVEDAA
                     RDFGARFVRAPVGEANVARSIRDEGAVIGGEGNGGVILPALHIGRDAPLGVALILHHL
                     AKIGTSLSRVVAAAPRYTILKAKAPRGRDLGPLFEGMRRRFPDAAADERDGLRLAWPD
                     RWLHVRPSGTEPIVRLIAEAPTAADAEQLVAAGRELLT"
BASE COUNT         2666 a         4881 c         4621 g         2559 t
ORIGIN      
        1 gacccacgcc cggcacgagc actcgatccg cagcccggac cacgtccgga tcagccgtga
       61 gcaccgagtc atggccgcct gcggacaatg cacgaaccac gctgcggagg ttgttcacgc
      121 cgtagtcgac cacggcgatc ataggacgcc cttggtcgac ggaacgccgg tcacccgcgg
      181 atcgagcgtc gttgccgcat ccatggcgcg ggcgaacgcc ttgaacgtcg cctcgacgat
      241 gtgatgagga ttgtctccac ggaccaggtc gatgtgagcg gtgagcccgg cattgagcac
      301 cagcgcacga aagaactcgg gggtgaggaa cacatcgtag tcccccagca tttgccactt
      361 ggggatgtcg acgtgatatg acaggtaggg acgtcctgac acgtctacca cagcgcgcac
      421 cagcgcctcg tccagcggga ccagggcgtc ggcatagcgg cgaacgccag cgcgatctcc
      481 caacgcttgc gcgatggcct ggccgatcgc aatgccggtg tcctcaaccg tgtggtgccc
      541 gtccacgtgc aagtcaccgc tgccctcgat cgtgagatcg atcagggcat gccgggcgaa
      601 cgcctcgagc atgtggtcga agaatccgat cccactgcgc acctgcgccg cgccggtgcc
      661 gtcgaggacg aggcggacat gcaagtccgt ctctttggtc tttcgcctga cttcgccgat
      721 ccggctcata agaaaagcgt aaacggtgga cgttcacgct ttcaagatct gccccagccc
      781 cgccaccacc gcctccatat cctccggtgt ccctaccgag atgcgcaggc actccgcgag
      841 ctccggggcg cccgatacat cacgcaccag gattccgtgc tcctcgagca gccggcggaa
      901 cacttcagcc gccggcagag cgacacagcg aatcaggatg aaattcgcgg cggatggaaa
      961 gactctgagt cccggagttg agttcagctg ctgaatagtc cggtcccgcg tcgcaatgat
     1021 ttcgggaacg tgggcgccgg cgagatccgg atgaccgagc agcacttcag cagcggtgag
     1081 tgaaagcacg ttgacgttgt acggaagctt ggccttggcg atctcgcggg caacgcttgg
     1141 gtgagccagc gcaacgccca accggagccc cgcaagccca agcgccttgg aaaacgtccg
     1201 caggaccacc acgcggggcg acgaggcaag cagccccagg gcggtggtgc cgccgaagtc
     1261 ctggtaggcc tcgtcgcaca gaacaagcgc gtcggtatcc tccaacaccc ggtccacgcc
     1321 atcgggcgga agcgcggagc cggtcgggtt gtttggcgag ttgagcacga tcacccgtgc
     1381 ccgctcggcg gttgcaacct cgctcaatgc ctccacgttg tatcggaaat cagcgccaaa
     1441 agccacgggc acgtagcatc caccgaaaac gttcgtgagc agccggtaga gggaaaatgt
     1501 cggcgtaggc gctacgacgc ggtcgccggt ttccagtgaa actgcgagcg tggcctggat
     1561 cagctcattc gacccattgc ccaccagaat tccgtccgtc ggccagttga agcgagtcga
     1621 gagctgagcc aggaggcgcg ccggaacaaa ctccggatag cgatgccacg aggcgccggc
     1681 catgcgctcc aggatctccc gcttgagctc cggcggcacg tcgtacggac tctcgttctg
     1741 attgagcttg cggcgggctt cgaacggctt cagagcgtaa gcaggttggc ccctgaccga
     1801 cggtttgatg agcgcgaggg ctggcccggg atcggcgagg ggctgcttca tcggccgcgg
     1861 cggccgagaa cggcttcgag ccgcgcggcg agtgcgcggg cgcggaacgg cttggagaga
     1921 aaatcgtcgg caccgagctc gaacaccttc acctcgttct cctcgtcgcc tttcgccgtc
     1981 agaaccacga tcggaatgtc cttggtcacc tggcgcgacc gtagctgcgc cagcacgctg
     2041 tacccgtcga gtcccggcag attgagatcg agcacaatga tgtccggtgc gtgccgatcg
     2101 acctcgtcga gcgcctgaat tccgtcccgg gcctcctgaa ccgtgaaccc ctcccgctcg
     2161 agtagatcgc gcaggacgcg gcggaggtcg tcttcgtcgt ccaccaggag cacacagata
     2221 ccccgcccac cctgggcgta accgtctgcc ggaggactct cgtccagcaa ctcgagatcg
     2281 aggccaaaat cgaaggcagg cggcgcctgg ggagcggcga ccggcggtgg cggtgccggc
     2341 ggcggcgcag ttgccccccg tcggcctgat cgaggtggtg gtggagggct caccggctcc
     2401 cggaccgagg tctgaggcac gtcggtgacc cgaagcagct cgtccagagt ggtttcgccc
     2461 ctgagcatgt gcctgagacc gctgtcccac agcgacttca taccgttgcg acgccccatc
     2521 tcggcgatcc gatccgccgt cccacccgac ccgatcagac gctccagctc gggattcatc
     2581 gccaggacct cgacgatgct gaaccggccg cgatagccgg tagatgcgca ttcgggacat
     2641 ccgacggcgc gatacagaac ggttcctgca ggtatgaact gagccagccg ctccggcacc
     2701 ggatctgagc tcacctcctt gcagatcgga cagagccggc gcatgagccg ctgagccacc
     2761 acgccgcgga gcgccgatcc gatcttgtac gcttccatgc ccatgtcgag caatcgggtg
     2821 acggcgttgg gagcgtcgtt ggtgtgcagc gtggagagaa cgaggtgccc ggtgagcgac
     2881 gcctgcacgg cgatttgcgc cgtttcctgg tcacgaatct cgccgatcag gacgacgtcc
     2941 ggatcctgcc gcagaatcga gcgcagcgcc gacgcgaacg taagattggc cttctcgtga
     3001 acctggacct gaacgatgtt cgcgcccagc cgatactcga ccggatcttc gacggtgacg
     3061 atgttgacgc cctcaccctg tatcactcga atcgcagagt acagagtggt ggtcttgccc
     3121 gagccggtgg ggccggtcac gagcagaatc ccttccttga aatgcagcag gcgacgaatc
     3181 tgatccgcct catcctcgaa caggccgagt ccatccaggg cgagaatggt ccctttgctg
     3241 ctgaggagcc ggatcacgac cttttctccc atggacgcgg gtagggtcga gactcgaaga
     3301 tcgatcggtt cgttgttgac ggtaattcgc gcccgtccat cctgcggccg cagccgatca
     3361 gcgatgtcga gcccggacat gatcttcact cgggagatca gcgggatgcc ggcggtgcgc
     3421 gggattctca tgacctggcg cagcacgccg tcgatccggt atcggatcac gacgccgcct
     3481 tccgcgggct cgacgtggat atcgctcgca cggctggttg cggcgtctgc cagcatcatg
     3541 tccaccaacc tgacgatggg tcgctggctc gcctgctccg cgctcgcggc agcatcgtct
     3601 tcgtcggcaa cttccttgac gtcgaagtcg ccgccgatcc cctcaaggag cctggacacg
     3661 acgtcgtcgt tccggtagag atcgtccagc ttttcccgga tccgcgccgg cgagccgagc
     3721 gccatgcgga cttcccgccc ggtggcgaac gcgagcgttt tttccgcgtc gaggtcgaac
     3781 gggttggcgg tggcgacttc gaggtaggcg tccgtcacct tgagaggcaa cacgttgaac
     3841 ttgcgaacca gttgctccgg gatctcatcc ttgacccgcg gatcgagccc gctgaagtct
     3901 gccacgggga gccgaaagcg cgacgcgatg gcgccgagaa tctgggcgtc ggtcgccagc
     3961 ttgcgttgca ccaccgcctc ccacaccgac gccggagcgc cctcggtccc gcgaagtgcc
     4021 ttcaaggcat cttcgggcac caatgtttcc agagtgggga ctagccattc gtcggtgaac
     4081 tgcatgaggc ggctcggctt gtcagaacgt ggcggacggg acacggcggg cgggcggcac
     4141 ccagccgaac aacataaggc cgggcgctgg cgacctggta ggtcagagca cgccccacca
     4201 gtccggatga acgtcgaagg tcaatccgaa atcaccatcg cgcagaccga ccccggcctc
     4261 gacacgcagg aggcgcaaga aaaattccag cgccacgccg gcgaccggcc ggaccccatc
     4321 tgttgccggc cagggcaatc catcgtacgg ccgttctgac caaccagccg caaaaaacgg
     4381 tgccagcacc atggtatggc cggtcgaggc gaacgaacca agcggaaaag cgggagccgg
     4441 gacgtcaacg cgccattcca gctccgccag ggcgtagctc cggcctccaa acccgcggaa
     4501 cggctcccct accagcgtgc ctcgaccgcc cagcacgaag ccgcgatatg caggaagact
     4561 gtcagttccc atgccgacct gaagcttccc tgccagggcg ctcctcccaa gtggagcgcg
     4621 ccaggaggca tccagcagca cgcgggcgta gctggtgtga ccttcacccg cttcgacagc
     4681 cagactgccg gtcacatcgg cggcgtccgc gaggcctccg ccggcccgct ccagtcgcgc
     4741 gcgcagcact ccataggtgc cggcgccaag gggtggattg ggacggtatg atccacgagc
     4801 cggagtcgct tccacgtcca aagaggtgct gtgctccaca cccgcctcga agccaagccc
     4861 aagtcgtgag ccgagtcggt gccggagccg tgcgccggcc gcgtcgagga gaacgtagtc
     4921 gccgtaatcg tctccgttct cctgggcgag caccgaattc agggccccgg aaatgacggg
     4981 gatatcgctc aggtctcgaa tccgccgctg tccgaagatc ccgagttcag ttgcaccgag
     5041 tccggcggtg agctcgatcc caccggtaac acgctgatcg gaggtgccaa aggcaatcgt
     5101 cgggcgaacg ccgaatcgcc ccccgccgaa atgcgcgatg ccgcccaggc cgagagtcag
     5161 accttgcacc cgattcacgt gaaccaggtc gctcacggag ccaaccgcga gccgcgttgg
     5221 gggaagcccg ctcagtcgct gggttgacgc gagtcgtgag atgtcgaccc gaagcgcctc
     5281 catatcctgt cgatcaatgg gggcagcttc tgcggcgatc gcgtcacgga gcgaagttgg
     5341 ccaggcgctg tccgtcggct gcggctcggt cagacccccg atctcggggc cttccagcac
     5401 cgagggaggc agcggcacgt tgaactcgta gtcaccgatc tcccagcgtc cgcggatgat
     5461 gcctcgaacc gggaagtcga accagctggc gcgccgccgg atctcgattt cctgccggta
     5521 cggcagccag aatcgccctt cctggagaga attctccagg accacgctga tgtcctcgag
     5581 gtttcgctcg agatacgccg ccggagtgaa gctgaaccgg aaccggacca gctcggcgga
     5641 ctctgcgtcc agatagaggc tgcccaccac gagcgggcgg tcgtactgcc gcgggcgaat
     5701 caggagctcg cgcaccttca cgtcgccacg aggtccccgg atcgtgagcg aatcgcccag
     5761 agaaaagtcg taggcatcga ggccgaccgg ggacagagga tggacggcgt cgcgaacctc
     5821 gtcgccctcg ccgatgcgga tcaagtctcc gaagttgttg gtcacaatgc ccaggtggtc
     5881 gcgatgatac gcgatgtccg tgggaagaaa tgatccgtcg cgccagccgt ggatgacttg
     5941 cttgctccga tttggtgcct gccagtacac ttccacgtcc aactcatcgg ctttgaccag
     6001 acgcggcggt ccgcccagtc cttccttacc gatctgggtc aggaagaaga cgaaaccgtg
     6061 ggctcgagta cggtagcttt ggagcgtcgg gtcagcctgt actgcttccc gccgctccac
     6121 accacgacgg gcgagcgtga gggcagcggc ggaattccag ccttcttgtg cggtgagcga
     6181 agagcagcga accaagagaa gtgagagcac cagagctact gcccgcggag cggtcaccgt
     6241 catggaagcc ggattccctc tcatctgccc tcgtgcgtca cggcgtggct tccgaatatt
     6301 ccgtggactc gccgccggga caatcgcgct cacggcggca tgtgccgctc cgcggcccgc
     6361 gatcacagtc aaaccgacgg acacgaccac cggggatccg tcccgcacct attccttgag
     6421 ccaggtcatt ctcctccacg tggacagcct gtcgcctgac gacacggtcg tgaaagttcg
     6481 acggggtcag ccccgggtta tcgtaatccg ccatccccag ccggatgagc tcgccttcgc
     6541 cgacctggca cttcccgctc aggcgtttga ctccgctgcc ggcgattcgg tcaccgtgag
     6601 cgtacacatc cgtcccggcc tgtacgggct ggatctcacg acttcggcac cgctcaagca
     6661 ggggaccctc acattcaagt acccggtcca ttttgccgcg ccggcggagg ctaccgctgc
     6721 ctttggctcc gatgtcgcct tcgagcacgt acttgccatt ggcaagctcg ggccgaacga
     6781 gacaatcgta tttcagcgct ccagcaggcc ggcgagcgac aatctgtctg tagcgctgac
     6841 gtctgcgggg agctacgtgg tcggagcgcc gaaatgatcg accgctttgc ctcatttgcc
     6901 gaacgagcgc gccgggctgc cggcatcacc ccgatcaccc gcgaagtcgt cctcgacggc
     6961 gacaccgctg tgaccgcgtt cagcaagatt caccgaggcc catacggatt tctgctggag
     7021 tcgctcgaag gcggtgagcg ttgggcgcgc tacacctttc ttgcgaccga cccacgagaa
     7081 gtcttccgat accgggggcg cgtctgcgag cgctggacct ccacaacggg ctggcagatc
     7141 gccgaccccg atgtggcacc gttggaccat ctgggccgca ccatgcgaca acacgcccct
     7201 gtcgaagtgc ccggattgcc ccgcttcacc ggtggggcgg tcggatatct cggctatgac
     7261 atcgtacgca cgatcgagtc actcccgagc ccgccgaagg acgatcgtgg actccctgac
     7321 gcgatcctga tggtcgcgga cactttgctc gtgctcgaca acgtgtacaa ccgcgccaca
     7381 gtaatcgcca acgtcgaggt tcccgcgtca gccgcggagc cggacctgcg tcgtttgttc
     7441 gagcagggcg aggcgcggat cgacgactgg ctctgccggc ttcaggcccc tgtaaccgca
     7501 ggaccgttgc cgctcgacct tcccgattca tctccgcagg ccacctcgcc atacgcggac
     7561 cacgactttc aggacgatgt gcggcggatc aaggagtaca tcgcggcggg agacacgttc
     7621 cagacggtcc tgtcgcgcag gctcgacctg tccgcccctg atcccttcct gacctatcgc
     7681 tatctccggg cgctcaatcc cgcgccctac ctttactacc tccaatgcga cgacatgtac
     7741 gtggtgggca gctcgcctga aatcctggtg cgagtggagg atggtgaggt cattctccgg
     7801 ccgattgccg ggacccgccc ccgcggcgag acgccggacc gggacgccgc gctcgcagcg
     7861 gagctggaag ccgatccaaa ggagcgagcc gaacacttga tgctggtcga tctcggccgc
     7921 aacgatgtcg ggcgggttgc agagttcggt tcggttcgcc tgacggactt catgaccatc
     7981 gaacgctact cccatgtcat gcacctagtg agcgaggtcc gcggcaaact gcggcccggg
     8041 ctcgacgccc tcgccgccct ggcggcctgc ttcccggcag gtaccgtatc aggagccccc
     8101 aaggtgcgag cgatggaaat catcgacgag ctggagccgg tcaggcgggg accatatgct
     8161 ggggcggtgg gctatgtcgg ctggggggcg caggtgctcg ataccgccat cgccctcagg
     8221 acctgcgtga ttcaaggcgg ccgcgcttgg gtccaggcag gcgccgggat cgtggccgat
     8281 tccgatcccg cggccgaatg gcgcgagacc gaggccaagg cgcgcgccgt catgctggcc
     8341 ctggcgcttg caagcagggc gcaaatgccc cggtaccctt agcttagacg cgatgttcag
     8401 ctatcggctc gtcagccttt caggcaacca gcgccacgat ctccccgccg gccggaccat
     8461 ggtcgtgggc cgcggggtgg gctgcgatat cacggtctac gatcccacca tctcgcgacg
     8521 ccacgcggaa ttacgggccg ggccggacgg agtcagcctg aaggacctgg gctcctcgaa
     8581 cggcacctac atcaacgggc gtcgagcgac caatgcccgg ttgagcgtca acgacactgt
     8641 cacgttcggg aaggtcggct atcagctgga agcagtcccg gtcgagcccg agcggccatc
     8701 ggcgtccgcg acaggagcta gcacgatcgt ccgggaagtg gctgccgccg gacccacctc
     8761 cggtgacgtc tccgcccgca agttgtccct cctgctttca atggctcaga agctcaccgg
     8821 cgagttcgac atcgatcgcc tgctggacac catcgccgca gtctcgttcg aagtcatgcg
     8881 ggtggatcga gtcgccattc tgctgcggca ggacaacacc ggcgagctga ttacccggct
     8941 ctcccgggcc cggctcggcg gcgctgaagt cgagcgcgtc tccggctcca tcgtccggaa
     9001 ggcgatggac gagcaggtcg cagtccttac ccagaacgca gccaccgatc cccggttcaa
     9061 aggccaatcg atccgcatgc agaacgtgcg gagtgcgatg tgctcgcctc tcatggcatc
     9121 cgccgacgaa gcgctgggcg tgctctacgt ggacagcgtg actgcggaga atgctttcac
     9181 cgacgaggac cttcagttcc tgattgcctt cagcgggctc gcggccgtcg cgattcagaa
     9241 ctcccgttat gccgagcaag cacgccgtga aacactggtc cggtcgaact tcgaacgcta
     9301 cttcgcaccc gacgtcgcgg cggacatcat ccgtcagaag actccgatcc agcctggcgg
     9361 cgatcggcgc gcgatgacca tcctgttcgc cgacattcgc ggcttcaccg cgattgcgga
     9421 aggaatgtca cccgatgcca tcgctgagat gctgaccgaa tacttcagcg agatggtgga
     9481 cgtgattttc gagcatggcg gcgtgctcga caagtttatc ggcgacgcct tgatggctct
     9541 gtggggctcg ccgctcgcac gggagcacga tccccggcgg gcactcgaag ccgcgcatgc
     9601 gatgcagcgg gcggtcgacg cgctcaatgg gcgctggcac gcggctggcc gtcccgagat
     9661 cggcgtcggc atcggcatca attacggtga ggtgttcgtc ggcaacatcg ggagcctgcg
     9721 acggctcgag tacaccgtga tcggggatgc cgtgaacgtg gcgcagcgcc tctcgagcga
     9781 ggcagccgca ggagagatcc tggtcagcga ggcctaccgg cgcgtctcgg gcgatgtcgt
     9841 ggatgccgag gagcctgcag ggctgggtct acgggggaag agccaggcgg ttgaagtgtt
     9901 tcgggtgcga cgggagcgtg ccagcgcaga tggaggcggc cgggagtaga cgggaacagc
     9961 tggaataaag cgcgcttgat cgactcggta gaatcaggca ggtccggtcc cggcccgttc
    10021 ctgaccaaag aagtaaggat gtcccgcccg ctccagcagc tggagcacca gccgaagcgg
    10081 cagtcccatc acgccgaaga agtcgccctg aatgccttcc accaacgccg caccgaatcc
    10141 ctgaatcccg taggccccag ccttatccat gggctcgccg gtagctacgt acgctgcgag
    10201 cagctcgtca tcagccggcc gaaacgaaac cgccgtcacg tcggtggccc cgagcgtgcc
    10261 tgccggtgtc ttgagagcga tcgcggtgat cacatgatgg gtccggccct ggaggcgacg
    10321 gagcatccga agcgcgtcgg cggcatctga tggcttttcc aacatctcgc cttcgaccac
    10381 gaccgtggta tccgctccaa gcaccagatc cccgctgacg ctggcagctt tctcccgggc
    10441 gagtcgctcg acgtatggct gcggcaactc gtctgctcca cggatttccg gaatgtgcgg
    10501 cggtcgaacc tcgacctcga gccccagcat ttcgagcagt tgcctgcgcc tcggggatcc
    10561 cgaggccagc accagcgtcg gcccgctcat gtcgatcgct ccagcacgag cgttaccggc
    10621 ccgtcgttga cgagctccac ctccatcatc gcgccgaaac gtcctgtcgc cacttcgagc
    10681 ttccgctcgc ggagcgcggc gacaaagctc tcgtagagtg gaatggcggt ttccggtcgt
    10741 gccgcatcta tgaacgatgg ccggcggccc ttctccgcat cactgtacag cgtgaattgc
    10801 gaaaccacga gaactttgcc gtcaacctcg gctagcccca gattcatctt tccctcggca
    10861 tcggagaaga gccgcagccc cgaaatcttc tcggccatcc actccacctg cgcggttgtg
    10921 tcgctgtggg taaagccgac cagcaggcag aatcccgccc cgatagaacc cactacgcgg
    10981 ccgtcgatct ttaccgcagc cctcgagacc cgctgaagca ccacccgcat tgtagtccct
    11041 ggtcgccact tggccggtcg cgcacgtacc cccggaggag gtcactaagt taatgcgtcc
    11101 ggtattctcg agtctcgagg aagtcatggc acagctttac aaaaccgatg agatagatcc
    11161 tgcaggctcc gaacagcgca gttgtcgagt gcgacctgcg cctcggcgtc cgcatcccga
    11221 ccgctggccc gatggtcagg cgacgttcgc ctggctgggt catgcgactg tcctggccaa
    11281 cttcttcggt gtgtggctcc tgaccgatcc cgcactcgaa gcgaggatcg gagtaggccg
    11341 gggacgtgcg aagctcgggc cccggcggct cactgccccc gcgctgcgac ccgccgagct
    11401 gccacagctc gacttgctgc tcctgtcgca cgcacacatg gaccacaccg acctgggtac
    11461 gctgcgttcg ctacggcgcg acgtgcctgt cgtcgtgcag ccgggcaata gcgacctggt
    11521 ccggcgcttt cggaaagtcg atgagctgcc ttggggagag cgagtgaccg ttgcgggaat
    11581 cgagatcgaa tcggttccgg tgcgtcactg gggtgcgcga atgatcaccg atcggcatag
    11641 agggtacggc gggtatctgc tgcggcgttc cggccggacg gttctgttcg ccggagacac
    11701 tgcgtacacc gacgcattcg ctccgctcgg ccggcgaggc ccgatcgacc tcgccatcct
    11761 tccgatcggc gcgtacgacc cctggatcgc aaaccacgca tcacccgagg aagcgtggcg
    11821 gatggcgcgt cagatgaacg cgactcacgt tctgccggtg caccattcca ctttccggct
    11881 cagtcgcgag cccatgcatg aaccgatcga gcggttgctg aatgctgccg gccccgaacg
    11941 cgatcggatc gtaattagag aaatcgggca gacctggacc ttgccggagg gcccggcaga
    12001 ctaagtagcg atttgcgcgt gggttacaag aacaagcccc ggggccaagg ctccggggct
    12061 ttagaatcga ccaaatcgat gccgatgtgg gggcgttatc ggttcgcccg ctactccacc
    12121 gtcaccgact tggccaagtt tctcggctga tccacattgc agccgcggcg cacggccacg
    12181 tagtaggaca gcagctgcaa ggggaggctg gtcaacatcg gcgtcaacag gtccaacgtt
    12241 tccgggatcg tgaaggtggc gtctgccagc cgctcgatct cggcatcacc cgggtttacc
    12301 agtgcaataa ccttgccacc ccgcgccttc acctcttcga tgttggacac gatcttcgag
    12361 tgcactgcgt ccttgggggc aatgaagaca acgggcatat tctcgtcgat cagcgcaatc
    12421 ggcccgtgct tcatctcggc cgcggggtac ccctccgcgt ggatgtatga gatttccttg
    12481 agcttcaggg ctccctccaa cgccaccggg aagttcacgc cgcgccccag gtagagcgcg
    12541 ttgtgaccgg tgctgaggaa ccggtcggcc agctgctcga cctcggatgc acgctcgagg
    12601 atattggcca tctgatcggg cagcttgccc agggcctgaa tgatctgccg gccttgcagg
    12661 atgctcagat tccgcagcct ggcgatacgc agggagataa gcgccaatgc cgctacctgg
    12721 ctagtaaaag ctttggtgct ggcgacgccg acctccggcc ccgcgtgcag atacaaaccg
    12781 ccgtccactt cccgtgcgat cgtcgagcca accacgttca ctacgccgat agttcgggct
    12841 ccacgtcgct tggcttcccg aatcgctgcc agcgtgtctg ccgtctcgcc ggactgggaa
    12901 atcccaacca caagcgtctt gttgtctacg accggattcc ggtaacgaaa ctcggaggcg
    12961 tactcgacct ccaccggcac gcgagccatt tcctcgagca tgtactcgcc gatcagaccc
    13021 gaatgccagg aggtgccgca ggccgtgatg atgatccgct ggatctgctt gagatcatcg
    13081 tccgagaggt tgagaccggt gacccgtgac gtcccttcgt cttcgagcag gtggccacgc
    13141 agcgtgttcc ggaggctctc cggctgctcg cagatttcct tgagcatgaa gtgggaataa
    13201 ccgccgcgct cgatggccgc gaggtcccac tcgatctgat tcaccggctt ttcgatctgg
    13261 gccgtctcca gattccggat gcggtatccg tcgcgcgaga ggatcgccat ctcgccgtcg
    13321 tccaagtaga tgaccgaccg agtgtgctgc agcaatggcg aagcgtcgga ggcgacgaac
    13381 cactcgtttt cccccacacc aaccagaagc ggcgagccct tcctggccgc gaccagcact
    13441 ccgggctcgt cggccgagac gaaagccagg ccgtaggcgc cgtccacgtc tcgcagcgca
    13501 gcagctaccg cttcctcgag atttccgcgg tagaactcgc caacgagatg tgccagcacc
    13561 tcagtatcgg tctccgactt gaaggtgtgg ccccgctgct ggagcgcctt tcgaatggcg
    13621 gcggcgttct cgatgatacc gttgtggatg acggcgatgc gccctgattg atcggtgtgc
    13681 gggtgagcgt tgggggtcgt gggcgcgccg tgcgtggccc agcgagtgtg tgcaatgccg
    13741 accgtcccgg cgggcagctc tccctcaagt tgctgctcca acaccgaaat cttcccggcc
    13801 gctttggcga tctcgaggcc cttgccgttg acgatggcaa tgcctgacga atcgtatccc
    13861 ctgtattcca atcgcttcag gccgtcgagc agcaatcccg ccgccggtcg agggccgata
    13921 tagccaacga ttccacacat ccgtgcgcgg tcccctaggt cagaagttct cggcccgcgg
    13981 ccaccagttg ctctgcatcg gcggccgttg gggcttcggc gatcaggcga acaatcggct
    14041 cggttcctga gggccgcaca tgcagccagc gatccggcca agcaagcctg agtccatcgc
    14101 gttcatcggc tgccgcgtcc gggaagcggc gacgcatccc ctcgaacagc ggccccaagt
    14161 cacggccgcg cggcgctttc gccttgagga tagtgtagcg cggcgcagcg gccacgaccc
    14221 gggataggga cgtcccaatc ttggccagat ggtgcaagat caaggccacc cccagtggtg
    14281 cgtcccggcc tatatgaaga gcgggcaaga tcacaccacc gttcccctct cctccgatta
    14341 ctgcaccttc gtcgcgtatc gatcgggcaa cattggcttc gccgacgggg gcacgtacga
    14401 accgggcacc gaagtcgcgc gccgcgtctt ccactaccag actggtcgac agattcacga
    14461 ccacaaccgg cgggccacca ttcgggttcc ccgtccccgc gagcacggcc cggacagcca
    14521 atgccagcgt gtagtcttcc ccgatcgcgt tgccttgctc gtccaccagg gcgagccggt
    14581 cgacatccgg gtccaccgcg aggccgagat ccgcgccgct ctcgcgcacc aggttaccca
    14641 gatcgcgcag gttttctgga atgggttcgg gcggatgggg gaaccgtcca tctgggtcaa
    14701 ggtggatgcc gcttacccgc acgccga
//
LOCUS       DASRJQ010000655         2382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1524728, whole genome shotgun sequence.
ACCESSION   DASRJQ010000655 DASRJQ010000000
VERSION     DASRJQ010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524728"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VFB89_14850"
     CDS             complement(<1..190)
                     /locus_tag="VFB89_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28624.1"
                     /translation="MTQTSRDGDALGFDHDFADYYDDAPPAPPAMLRAATPINGSPTV
                     RSSKSAQPTHPALPALPRA"
     gene            complement(187..555)
                     /locus_tag="VFB89_14855"
     CDS             complement(187..555)
                     /locus_tag="VFB89_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28625.1"
                     /translation="MRLRTDDDIYRARLVYLGPPGYTLPVQLPYAQYGVFIFLVPLFM
                     GIHYLITLEFDPVPAWEIALAMVTTSFIWRYVDPDRPARIVIRTALTDWRGAGRSASG
                     QEERAPRLVARRVTIREKIR"
     gene            complement(579..911)
                     /locus_tag="VFB89_14860"
     CDS             complement(579..911)
                     /locus_tag="VFB89_14860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28626.1"
                     /translation="MIVSTLAQLAGETLLPQARDLAHTVLAVEPSPAPDSPVPINTDG
                     IVQFIATKVTPILLAVLGVIFISRAGKGEMSKVLTSSAIAIVGLAFIAGAGALFFFGE
                     GLIKLIFG"
     gene            1042..1983
                     /gene="folP"
                     /locus_tag="VFB89_14865"
     CDS             1042..1983
                     /gene="folP"
                     /locus_tag="VFB89_14865"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HZM28627.1"
                     /translation="MPSRHQWGTTHLAGQVDLSRNRHWSTEYRRGVVKPIAPAPVVMG
                     VLNVTPDSFSDGGRYASLEDAVRHAVTMRTDGADVVDVGGESTRPGAGRVDAATECAR
                     VLPVLRELAAVGVPTSIDTTRAEVAEAALAAGASVINDVSGGLADPRMAAVVASAGVP
                     WVLMHWRGHSVQMQELARYRDVVAEVRDELRQRADAAIAAGVDAASIIVDPGLGFAKL
                     PEHNWQLSAHLDAIISLGYPVLVGASRKSYLGTLLADPTGAPRPVAEREAATVATSVL
                     AVQAGAWGVRVHDVRATVDALRVLQATREARLGRVHE"
     gene            1976..2350
                     /gene="folB"
                     /locus_tag="VFB89_14870"
     CDS             1976..2350
                     /gene="folB"
                     /locus_tag="VFB89_14870"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HZM28628.1"
                     /translation="MSDHMCLRGLRVHGRHGVYEFEREEGQEFLIDVDLELDLAAAGA
                     SDDVADTVHYGELADRLVAIVAGEPVNLIETLAERLASACLTDGRVAAVTVTVHKPSA
                     PIPHTFADVSVTVRRLQPRART"
BASE COUNT          420 a          727 c          824 g          411 t
ORIGIN      
        1 aggcgcgcgg caaggcaggc agtgccggat gtgtcggctg tgcggacttg gagctgcgca
       61 cggtcggcga tccattgatc ggtgtggcag cgcgaagcat cgcaggtggt gccggcggag
      121 cgtcgtcgta atagtcggcg aagtcgtggt cgaaaccgag cgcgtcgcca tcacggctcg
      181 tctgggtcac cggatcttct cccgaatggt gacccgccgg gccaccagcc ttggcgctcg
      241 ctcttcttgg ccggaggcgc tacggccggc gccgcgccag tcggtaagcg cggtacggat
      301 gacgatgcgg gctgggcgat ccgggtccac ataccgccag atgaaagacg tggtgaccat
      361 cgccagcgcg atctcccacg ctggcaccgg atcgaattcg agggtaatca ggtaatggat
      421 tcccatgaat agcggtacca ggaaaatgaa gacgccgtat tgcgcgtatg gcagctgaac
      481 cggaagcgtg tatccgggtg gcccgagata gaccagccgt gctcggtaga tgtcgtcgtc
      541 agtccgcagt cgcatcgtgt tctacccgcg atctcgttct aaccgaagat cagtttgatc
      601 agaccttcgc cgaagaagaa gagggctccc gcccccgcga tgaacgcgag tccgacgatg
      661 gcgatcgcgc tgctggtgag gactttggac atctcgccct tgccggcccg gctgatgaag
      721 atgacgccga gaacggccag caggatcggg gtcaccttgg tggcgatgaa ctgcacaatc
      781 ccgtcggtgt tgatcggtac gggagagtca ggggccgggg agggctcgac cgccagaact
      841 gtgtgcgcta ggtcgcgcgc ttgcggcagg agtgtctcgc cggccaactg ggcgagcgta
      901 gagacgatca ttggtatcct ccccagagca cgtggtccct agcctccccg agaagggttc
      961 cgcgcggaac cacgatgttg gcctctgtgc cgaaccaatg cggaggcagg gccacactga
     1021 cgcataagaa tattgcagta cgtgcccagt cgtcaccagt ggggtaccac tcatctggct
     1081 ggccaggtcg acctgtccag gaaccgacac tggtcgactg agtaccgtcg aggcgtggta
     1141 aagccgatcg caccagctcc cgttgtcatg ggggtcctta acgtgacccc cgacagcttc
     1201 tccgacggag ggcggtacgc cagcctcgag gacgcggtac gccatgccgt caccatgcgc
     1261 acagatggcg ccgacgtggt tgacgtcggt ggcgagtcga cccgccccgg agctggcagg
     1321 gtggacgcgg ctacggagtg cgcccgggtg ctgccggtgc tccgggaact tgcggcggta
     1381 ggggtaccaa ccagcatcga taccacccgg gccgaggtag ccgaggcagc cctggccgcc
     1441 ggagcgagcg tcatcaacga cgtctccggt ggccttgccg atccgcggat ggcggcggtg
     1501 gtcgcgagcg cgggcgttcc gtgggtgctg atgcactggc gcggacactc cgtgcagatg
     1561 caggagctgg cacggtatcg cgatgttgtt gccgaagtcc gggacgagtt gcggcagcgg
     1621 gcggacgcgg cgatcgcggc gggcgtcgac gccgcgagca ttatcgtgga tccgggtctc
     1681 gggttcgcca agctgcccga gcacaactgg cagctgagtg cacacctcga cgcgatcatt
     1741 tcgctcggct acccggtact ggtaggtgcg agccgcaagt cgtaccttgg caccctgctc
     1801 gcggacccga ccggagcgcc tcgtccggtc gccgagcgcg aggcggccac agtagccacc
     1861 agtgtgctcg cggtgcaggc tggcgcgtgg ggggtgcgcg tgcatgacgt acgtgcgact
     1921 gtcgatgccc ttcgagtact ccaagccacc cgggaagctc gactcgggag ggtgcatgag
     1981 tgaccacatg tgcctgcgtg ggctgcgcgt ccacggccgg cacggcgtgt acgagttcga
     2041 gcgcgaagag ggacaagaat tcctcattga cgtcgatctc gaactcgatc tcgcggcggc
     2101 cggagcttcg gacgatgtcg ccgacacggt gcactatggc gaactcgcag atcgactggt
     2161 ggcgatcgtt gctggcgagc cggtgaacct gatcgagaca ctggccgaac gtcttgcgtc
     2221 cgcatgcctg acggatggcc gggtcgccgc ggtgaccgtg accgtacaca agccttccgc
     2281 gccgatccca catacattcg cagacgtctc tgtgaccgta cgacgccttc aaccgcgggc
     2341 acgtacgtga ccagtgcaat tctctcgatc ggcagcaatc tc
//
LOCUS       DASRJQ010000656        12738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2371712, whole genome shotgun sequence.
ACCESSION   DASRJQ010000656 DASRJQ010000000
VERSION     DASRJQ010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 12738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12738
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2371712"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..1210
                     /locus_tag="VFB89_14875"
     CDS             158..1210
                     /locus_tag="VFB89_14875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28629.1"
                     /translation="MNRGWPVLAPLVSCLAVTAGLAQSSGGKAEVRRLFREETPLVLT
                     LNADFREVSANRDTVNKNRFAARLTFRGDSGPQSIPVELATRGHFRLRSSTCGFPPLR
                     VFFSKDQVKGTPFAGNGSLKLVTHCDKNQRYEQNLLVEYGIYRALNRLTDWSHRARLV
                     HVTYADSRDSTKNLTRWGFFLEADGDMAKRNGGQLFKSFGTYSEMDSTQMDLVGVFEY
                     LIGNTDWSVIMGHNIELVQIPGQSILYPVPYDFDFSGLVDAGYARPDPRMPIKSVRQR
                     LYRGMCRTPERIAPTLARVLAARDTINQAFATLPDLDPKRLKDVQKYLDDGFKAFATP
                     KSFMPEQDYVCSKTRE"
     gene            1279..2277
                     /locus_tag="VFB89_14880"
     CDS             1279..2277
                     /locus_tag="VFB89_14880"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28630.1"
                     /translation="MGVLASQADLLRQRYDIVFQVVGAADSTGAAVDTGGLDPAALLE
                     AKRRGSVAGLPSSGRPGMSAVELVQSTRATVVLESTPVNLRTAQPGLDVCRTALHRRM
                     AVVLANKGPLALAYEELVGLSDLTHSRAPALRFSACVGGALPTVDLGRRVLAGARVLK
                     VEAVLNGTTQFILREMEAGRTFGAVLAEAQRRGLTETDSSLDVDGWDAAAKLVIVANA
                     VLKQSATIRDVEVEGIGSVTPEILAAASARGERVVLLCLADPAGTDDGRFRLRVGPMA
                     LPRAHPLARMDGEEMGVVYYTDIAGRATALTLEHDPIPTASAMLRDLLDVVGAIPN"
     gene            2401..2814
                     /locus_tag="VFB89_14885"
     CDS             2401..2814
                     /locus_tag="VFB89_14885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28631.1"
                     /translation="MSRGSMRLGVVGSGCCALWLFTALVSGCGPRHAADNEVAPDPNE
                     PTPLTVRNDNLLDAIVFVYHDGQVTRVGTVTAASSANFMLAPWLLGPTRNINLAAHPI
                     GSPTSINTELIHVQVGQFIEWRLENDLATSSVSVY"
     gene            3322..4125
                     /locus_tag="VFB89_14890"
     CDS             3322..4125
                     /locus_tag="VFB89_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28632.1"
                     /translation="MKSLTPVTIAAGILSFAARPASGQIDYRNLDDGRPVATEDAYSI
                     ERFGFELLAPYRFEADHAGANSHVVAPELEYGLLPNTQVGIRTLLAAVSDAGTTDWGL
                     AGLRVFGLYNFNTESAGLPGLAVRAETAIPVGQLAGDAFSLALEGIATRSWGATRAHI
                     NAGVRVGTDTDISSVDTPPRWQVSGAIDHTFLRQSLLVIGELLTERETNGAPVEVNLS
                     AGLRWQWQPTLVLDFGLSRRLASNGPDLGLTVGLSHAFAVRSLIPGAGR"
     gene            4122..5150
                     /locus_tag="VFB89_14895"
     CDS             4122..5150
                     /locus_tag="VFB89_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28633.1"
                     /translation="MIRTRWSGGRVLAAALTLVAAGGLCRKADAQEGRDEQFYYPGSF
                     NWQFLRRYPDAARLFNAFDYGHAVLYEVLFTRKGDSGRDRLAEEFRYLTTDLLVRPPR
                     FAIAEEAVMPSYAKSAWRAKEAFDWAHLLHRQIYDVYADPRIESAAKDSLIERLTDYY
                     LSRVGYAFSARPKSMALMDEQYFSQTFRRFDPRFNGLIWAYHWLQVGLYEPFVRYRTE
                     PEQKAAVQRVLSRFWAMLRDPPSRMPRAMPMAATIAPEFSRRHPRAAAIFDNLHMMHD
                     IISDILASDSVPLENKKDVIYAQLRELGDSTRQVMTWEEWHHMGHMMGGADVMGGPVF
                     DDETTAPR"
     gene            complement(5228..5578)
                     /locus_tag="VFB89_14900"
     CDS             complement(5228..5578)
                     /locus_tag="VFB89_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28634.1"
                     /translation="MANRTWMEEDVWWEQEFPSRPYAVDRTYDEFRPAYRYGYESGTH
                     YMGRSWEDAEDDLRSGWDRFEGKGPGGSLWEDVKDAVRDAWNRVTGHHGLDADRMSES
                     EVDRLSHGGRPRNH"
     regulatory      5894..5984
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            6380..7264
                     /locus_tag="VFB89_14905"
     CDS             6380..7264
                     /locus_tag="VFB89_14905"
                     /inference="COORDINATES: protein motif:HMM:NF025464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparin lyase I family protein"
                     /protein_id="HZM28635.1"
                     /translation="MSDGTTKTLLPSYTATGGTISSTGLYTGGKTVGGFRVIAKDAGG
                     KADTSNVTISSSTSSTSATFVGEFETGSLSQYATAQQCAPSRIKVYSAASQPTWPAPR
                     QGSYAARFNVYDTDVYPCTSTQNPRAQIGSSNFIKEGDEYWVAFSVYFPTNLPDVTPY
                     DWVVIQSFYGSPGGGSGPTHFVLGWDGRLGKTVLSYPETGLAIPLVRGQWLDLMAHVK
                     FSKNPSLGWVEVYYNGVQQKLPACNCMRMVKQTINSETTSALHSKLAFYRKAGMWPGA
                     VAVFFDRHRVGLTRASVE"
     gene            complement(7410..8942)
                     /gene="crtI"
                     /locus_tag="VFB89_14910"
     CDS             complement(7410..8942)
                     /gene="crtI"
                     /locus_tag="VFB89_14910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HZM28636.1"
                     /translation="MTQVLALSRGNQPHSRASTPVRVLVVGAGIGGLAAAIRLAHAGY
                     AVTLVERHAEPGGRAGVWKSKGFTFDTGPSMVMMREYWDELFTAVDRRLDDYITLIRC
                     DPCYRLHFADGSRLEITSDLPQLIANFERIEPGCGPRALEWLAHTGELYRRGIGFIRR
                     GMDRPSAVLQLGQMGLRYGRAALGDLQAMTRRFFRDERLRQAVTFQTLYLGLSPYASP
                     GIYGLLAHTEIAGGIYYPIGGMHQLATALEAVGKELGVQYHYDADVTRLATEAHRVTA
                     ARLADGREIKADIVVANADLPYTYTHLLGEPYPRIDRKKFSCSVVLLCLGVNRTFPDL
                     LHHNLVVSSDLLESCRLLCDEHRMPADPSFYVVATTRTDPAQAPPGCESLFVLVLAPS
                     QDPDHPVDWSVEAPKVEQRTLERLEALGCRDLRQHIVTRQLMTPADFTGKFGNLRGEA
                     FGLSHGLTQIGYFRPRNRHAQYRNLYFVGQSTHPGCGLPMVLISAELVTGRICREIPR
                     GR"
     gene            complement(9098..9484)
                     /locus_tag="VFB89_14915"
     CDS             complement(9098..9484)
                     /locus_tag="VFB89_14915"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HZM28637.1"
                     /translation="MKLGIALRNALLVTALVSCSSNTTGPVVQPPTGGGGSNGSVVDV
                     SIRDFTFTPASVTVKAGTTVRWTNNGPSAHTTTSDMGVWDSAVLSPNAGTFEMTFSTT
                     GTFPYHCSIHPPSVYPGFVGTITVTQ"
     gene            complement(9681..10463)
                     /locus_tag="VFB89_14920"
     CDS             complement(9681..10463)
                     /locus_tag="VFB89_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HZM28638.1"
                     /translation="MTTERPFLTAAWRHLAMLNYEVPRSLLEPLVPAGTELDTFDGAV
                     LASVVGFRFLDTRVLGIAVPGHRDFDEVNLRFYVRRRGEDERWRRAVVFVRELVPRRA
                     VAVVARWCYNEPYTVVPMRHELALEGKGDGAPGRAAYFWQLGGRWHSLEVRTVGGAAL
                     PQQSSEAEFVTDHYWGYTAQRDGGCKEYQVVHPPWRVWQAVSAEFDCDVHLVYGAGFA
                     ECLTGPPRSAFLAEGSEVSVYRGRRLDEQEKLSRRRTAHTDR"
     gene            complement(10542..11108)
                     /locus_tag="VFB89_14925"
     CDS             complement(10542..11108)
                     /locus_tag="VFB89_14925"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HZM28639.1"
                     /translation="MPSAFSHAVAALGIGALFYRPEIPKRVWVVGAACAAVPDLDVIG
                     FRVGIVYGDVLGHRGLSHSLLFAAGLAAVVTFSGFRRGVPGLRATALWAYLFLATASH
                     GFLDAFTDGGRGVAFFAPIDNTRYFFPIRPIRVSPLTPRGFFSARGLAALRTELPWVW
                     LPSALLAVLASAWRRLPRWHGHRNQPGE"
     regulatory      complement(11266..11381)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            11617..>12738
                     /locus_tag="VFB89_14930"
     CDS             11617..>12738
                     /locus_tag="VFB89_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28640.1"
                     /translation="MPRTFPLVLGWMVSIGSACGGNDAAAPTSGTAERTPLAEVRTLS
                     GSIIWDAKSQMKQVASTKGCDSWTPTWAPDGNLYSGYGDCQMSGVPVKLGMGFARISG
                     STAYGVSVAAVPTGDPANWNDAATGAGVDATGNGTASEKPAGMLQIGTRLWALIRNIN
                     SGGHGVRLKYSDNYNTTSPDFTWVSWTFTELGYASFVQYGKGYAGGPTSYVYAVVPMR
                     SSSTGSVSNSAYDSVPAFGLLRGARTDLRIRTNWKYFCGPASTPSWCSASSSAKNVYF
                     KSGTKFRPRPGMSWNPGLGRFMLTLVYDATPDTSNTHDTRFAGSLMVLTAPNPWGPWT
                     TVYSSGSNPWPGGTPNTVGCGGSLNRWGAGDRADIPTKYM"
BASE COUNT         2334 a         4040 c         4038 g         2326 t
ORIGIN      
        1 cgagcctttc acagcgtctg acggccgagc ctgccgtctc cgttggcgcc gagccgccgg
       61 gcgcgttcta acggcgactc ccgaacgttc ctgaagcctc gcggaaccgt ggacggcaga
      121 gtagcataga cgttcgaggc ccacccgaga caagctcatg aaccgaggct ggccggttct
      181 cgctcccctc gtctcctgtc ttgccgtcac cgctggattg gctcagagca gtggcgggaa
      241 ggctgaggta cgacgattgt ttcgcgagga gaccccgctc gtcctgacgc tgaatgcgga
      301 cttccgggaa gtttccgcga accgcgatac cgtcaacaaa aatcgctttg ccgcccgtct
      361 cacgttccgg ggtgactccg gcccgcaatc gattccggtc gagctggcca cgcgcggaca
      421 ttttcgactg cgcagctcca cctgcggttt tccgccgcta cgggtcttct tttccaagga
      481 tcaggtcaaa ggaacgccgt tcgcgggcaa cggttcgctc aagctggtca cccactgcga
      541 caagaaccag cggtacgagc agaacctcct ggtcgaatat gggatctatc gtgcgctgaa
      601 ccggcttacc gattggagtc atcgagcccg acttgtccac gtgacctacg cggactcccg
      661 agattcgacc aagaacctga ctcgctgggg gttttttctg gaggccgacg gggacatggc
      721 caagcgcaac ggcggccaac tcttcaagtc gttcggcacc tattccgaga tggattcgac
      781 ccagatggac ctggtgggtg tcttcgagta tctgatcggc aacacggatt ggtcggtcat
      841 catggggcac aacatagagc tggtgcagat cccggggcag agcatcctct acccggttcc
      901 gtatgacttt gacttttcag gactggtgga cgcaggctac gccaggcccg accctcgaat
      961 gccgatcaag agcgttcggc agcggcttta tcggggcatg tgccgaacgc ccgaacggat
     1021 agcgccgact ctggcgcgag tgctggcagc acgagacaca atcaaccagg cgtttgcgac
     1081 cttgccggat ctcgatccca agcgactgaa ggacgttcag aagtatctgg acgacggatt
     1141 caaggcattt gcgacgccca agagcttcat gcctgagcag gactacgtgt gctccaagac
     1201 ccgcgaatga tctagacccc actgcctcac cttcactctc tgatactggt tggcctcggt
     1261 aacgttggcc gtagtctgtt gggggtactg gcctcgcagg ccgatctcct gcgacagcgc
     1321 tacgacatcg tcttccaggt ggtcggtgca gcggactcga ccggggcagc cgtcgacaca
     1381 ggcggcctcg acccggctgc gctgctcgaa gcgaaacgtc gcggcagtgt ggccggtctc
     1441 ccttccagcg gccgaccggg gatgtcggct gtcgaactgg ttcagagcac gcgagcgaca
     1501 gtcgtgctcg agtccacccc ggtcaacctc cgcaccgcgc agccgggcct cgatgtgtgc
     1561 cggaccgcgc tgcaccgcag aatggccgtc gtgctggcca acaagggccc cctcgcactg
     1621 gcatacgagg agctggtcgg actgagcgac ctcacccact cgcgcgcacc ggcgcttcgg
     1681 ttcagcgcgt gtgtcggcgg cgcactcccc acggtcgatc ttggccgtcg ggttctggcg
     1741 ggcgcccggg tgctcaaggt cgaagccgtg ctcaacggaa ccacacagtt catcctgcgg
     1801 gaaatggaag ccggacggac gttcggagcg gtcttggctg aagcgcagcg acggggcctg
     1861 actgagaccg attcatcgct cgatgtagac gggtgggacg cggcagccaa gctcgtcatc
     1921 gttgccaacg ccgtcctgaa gcagtcggcg accatccggg atgtcgaagt ggaagggatc
     1981 ggaagcgtga cgccggagat cctcgccgcc gcatcggcca ggggcgagcg ggtggtcctt
     2041 ctttgcctcg cggacccggc gggaaccgac gacggtcgtt ttcggttgcg ggtcggccca
     2101 atggcgcttc ctcgcgcgca tcccttggct cgcatggatg gcgaggagat gggtgtggtc
     2161 tattacaccg acatcgcagg ccgtgccacg gctctgacgc tcgagcacga tcccattccc
     2221 acggccagcg ccatgcttcg cgacctgctc gacgtcgtcg gcgcaattcc aaactgaggg
     2281 cagcgcctct cgctcccggt cttgcgccgg gaagaacgga aagtctaacg tttcgtggag
     2341 tgactgttgt caccgcccgg aatcgagtcg acggtgtacg cttgggcgaa cagttgattg
     2401 atgagtcggg gttcaatgcg gctcggcgtg gttggctccg gatgctgtgc gctctggcta
     2461 ttcaccgctt tggtgagcgg atgcggcccc cgccatgcgg ccgacaatga agttgctccc
     2521 gaccccaacg agcccacacc gctaactgtc cgaaacgaca atttgctgga cgccatcgtt
     2581 ttcgtttacc acgatggcca ggtgacgcga gttggaacgg tgactgcggc ttcgagcgcc
     2641 aacttcatgc tcgcaccgtg gctcctgggg ccaacccgca acatcaatct ggccgcacat
     2701 cctataggat cgcccacctc gatcaatact gagttgatcc acgtccaggt gggtcagttt
     2761 atcgagtggc ggctggagaa cgacctcgcg acgtcttccg tctcagtcta ctgatcctag
     2821 cccctgagct tctcattgat tctgggacag ctagccgccg agcacgcagg ctcgctacgt
     2881 ttccagcatg ccggttaccc ttcgcctgct tcctcatttg gggcgtgttg ctcgcggcct
     2941 ggtcttgctg tcggcgttct gcctcggcct cgccgtccag ttcctgggtg acagccgctc
     3001 gcattgccgg cgccatgcgg agcatggacg ctcccacgct gcctcggccg tcgtggtcgc
     3061 ggcatcggcc ccgtcgtgcg accattgtcc taatccgcag tgcggcgtga cagtctcgtg
     3121 tgcgggggga tcgagcaccg gcatagtggt ctcgggcccc agggtggtct gggccgagcc
     3181 tcgccatctg atcgatgatc ggcagccggt cgtgctcggc tcgcggacac acccaccttt
     3241 aaccccacca cctcaagcga tcgcgtagtc ccccgccgag caacctgcac ggcggtcagc
     3301 atcgactgcg aggatcgtat catgaagagc ctgaccccgg tcaccattgc ggccggcatt
     3361 ctttcgttcg cggcgcggcc cgcgtccgga caaatcgact atcgaaatct tgatgacggg
     3421 cgcccggtcg ccaccgagga tgcctattcg atcgagcgct tcggcttcga attgttggcc
     3481 ccctaccggt tcgaggccga tcatgccggg gcgaacagtc acgttgtcgc gcccgaactc
     3541 gaatacggcc tcttgcccaa cacgcaagtc gggatcagga ccttgcttgc tgcagtcagc
     3601 gatgccggca ctaccgattg ggggctcgcc ggactgcgcg tgttcggtct ctacaacttc
     3661 aataccgaaa gcgccgggtt gcctggtctg gcggtgcgag ccgagacggc tatccctgtg
     3721 gggcagctgg ccggcgacgc ctttagcctc gcgctggagg gaatcgccac ccgctcctgg
     3781 ggcgcaacgc gagcgcacat aaacgccgga gtccgggtgg gcacagatac cgacatcagc
     3841 tctgtggata cgcctccccg ctggcaggtg agcggagcca tcgaccacac gtttctgcgc
     3901 cagagcctgc tggtcatcgg ggaactgctc accgaacgcg agacaaatgg cgcgcccgtc
     3961 gaggtcaacc tttctgccgg gctccgatgg cagtggcaac cgaccctcgt actcgacttc
     4021 ggtctgagtc gcaggctcgc gtccaacggc cctgacctcg gactcacagt gggactctcg
     4081 cacgcatttg ccgttcggag tctgatccca ggagccggcc gatgatcagg acacgttggt
     4141 ccggcgggcg tgtcttggct gcggccctga ctcttgtggc tgcaggtggc ctgtgtcgga
     4201 aggcggatgc gcaggagggc agggacgagc agttctatta ccccggctcg ttcaactggc
     4261 agtttctgcg tcgctaccct gacgcggccc ggctattcaa cgccttcgac tacgggcacg
     4321 cggtgctgta tgaggttctc ttcacccgga agggtgattc ggggcgagac cgtctggccg
     4381 aggaatttcg atatctgacg accgacctgc tggtgcgtcc gccccgcttc gccattgccg
     4441 aggaagcggt catgcccagc tacgcgaaat ccgcgtggcg tgccaaggag gcgttcgact
     4501 gggcgcatct gctgcatcgc cagatctacg acgtgtacgc cgatccgcgc atcgagtccg
     4561 cggccaagga ctcgctgatc gaaaggctca ccgactacta cctgagccgg gtaggctacg
     4621 cgttttcagc ccgacccaag tcgatggccc tgatggatga acagtatttt agccagacct
     4681 ttcggcggtt cgacccgcgc ttcaacggat tgatctgggc ctaccactgg ctccaggttg
     4741 ggctctacga acctttcgtc aggtaccgga ccgagccgga gcagaaggca gcggttcaga
     4801 gggtgctttc ccggttctgg gccatgctgc gagatccccc ttcgcgcatg ccacgggcca
     4861 tgccgatggc ggccaccatc gcgccagagt tctcgcggcg ccacccgagg gcggcggcca
     4921 tcttcgacaa cctccacatg atgcacgaca tcatttcgga tatcctggcc tcggattcgg
     4981 ttccgctgga gaacaagaaa gatgtgatct atgctcaact gcgggagctg ggtgactcta
     5041 ccaggcaggt gatgacgtgg gaggagtggc accacatggg ccacatgatg ggtggggcag
     5101 atgtcatggg gggtccggta ttcgacgacg aaacaaccgc tccgcggtag agccgggcag
     5161 tagaaagggc cgcctgccgg cggccccgtt tgctgcttgg tcagtgagcg ccggaccggc
     5221 accgccccta gtggttccga ggccggcctc cgtgtgaaag acgatctact tccgattcgc
     5281 tcatccggtc agcgtcgagc ccgtggtgtc cagtgacccg gttccacgca tcccgtacgg
     5341 cgtccttcac gtcttcccac aacgacccgc ccggtccctt gccctcgaag cggtcccagc
     5401 cggaccgtag atcatcttcg gcgtcttccc agctgcgtcc catgtagtgg gtacccgatt
     5461 cgtagccgta gcggtacgcc ggtcgaaact cgtcgtatgt gcgatccacg gcatagggtc
     5521 gagaagggaa ctcctgttcc caccaaacat cttcttccat ccatgttcga ttggccatga
     5581 catctccctg acgactgtac aaaggttcct gcttcgcggt cacacgttag tcggggcgag
     5641 tcgggatggg agtgatggtg cccacgatac tagacccacc gtggccacgg taaatcgtgg
     5701 gtcgacaccc gttgcaatcg agttcctgtt tgttttcttg acggttatcg tcgtgtttcg
     5761 aacggcctaa tgcgtttcta tgctattgta ccgcaataat ttcatcatac gacccgaccc
     5821 catggcccat taattgttat acgccgggcc gaagtccatc aaatcgggcg ccgccagagg
     5881 cgccaccagt caacgctgct cagagtaaat ccgccgaaag gcgggggcac agagcttcgg
     5941 ggctaccgcg atccattgat cgtcatgtca gccgggccgc cagcgccggt tcctcgattc
     6001 ggggaccgac gactggcgtt ttcaatgcct aattctggga gtcggcttca tgacgtacgg
     6061 gtacaaactc gctcgacgga tcgctcgcct tcgcactgcg cctcttgcac tgattgccgg
     6121 agtcgccgcc tgtaccggcg ccgagccaac cggtcttggc agcgacgatg gaaacgcttt
     6181 gcaagtcccg ggcaacgccc cggtccccgc gtcggccccg tcgccggcgg acacgatcaa
     6241 acccattcca gcgtcaccgt ccagtaccgg cacccccgga gtcagcagca caacagcgac
     6301 gctgacctcg gttgtcataa gccccgcaag cgcgtccctc gctcccggga agacccaaca
     6361 gttcaaggcg acagggaaga tgtccgacgg cactaccaag acgctgttgc cgagctacac
     6421 cgcaaccggt ggaacgattt caagcaccgg cctgtatacg ggcggtaaga cggtcggggg
     6481 ctttcgcgtg atcgcaaagg atgccggtgg gaaggctgat accagcaatg tgacgatcag
     6541 ctcgtctacc tcgtcgacgt cggcaacctt cgtcggcgag tttgaaaccg gcagcctgag
     6601 ccagtatgcg acggcccagc aatgcgcacc cagccggatc aaggtgtact ctgccgcctc
     6661 gcagccgacc tggccggcgc cgcgccaggg cagctatgcg gcccggttca acgtttacga
     6721 cacagacgtg tatccctgca cctcgacgca gaatccgcgg gcccaaattg ggagttccaa
     6781 ttttatcaaa gagggcgacg aatactgggt ggcgttcagc gtctatttcc cgacgaacct
     6841 acccgatgta acgccatacg actgggtagt catccaaagc ttctacggta gtccgggtgg
     6901 tgggtcgggg cccacacact ttgtccttgg ctgggacggc cgtctcggga agacggtcct
     6961 gtcgtatccg gaaaccggcc tggctattcc gctggtgcgc gggcaatggc tcgacctgat
     7021 ggcgcatgtc aagttttcca agaatccgag tctcggctgg gtcgaggtct attacaacgg
     7081 tgtccagcag aagctgccgg cgtgcaactg tatgcggatg gtgaagcaga ccatcaactc
     7141 tgagaccacg agcgcgctgc actcgaagct cgccttctat cgcaaggccg gcatgtggcc
     7201 gggcgccgtg gcggttttct tcgaccggca tcgggtggga ctgacacggg cttcggtcga
     7261 gtaagctctc gaacgcgctg atccgccgct gctacggcct gacggagaca aaaccaacgg
     7321 gtaacagaga gccgcacggt ccgccctccg ggcgggtcgt gcggtatcgt tgcgaggcaa
     7381 gctgcggcga tttcatacgg gaatcgagct catctgcccc tcggtatctc tcggcagatt
     7441 cggcccgtga cgagctctgc agagatcagc accataggta gtccgcaccc gggatgagtg
     7501 ctctgaccga cgaagtagag attccgatat tgggcgtgac gattccgggg gcggaagtat
     7561 ccgatctggg tcagtccgtg ggaaagtcca aaggcttcgc cgcgcagatt cccgaacttc
     7621 ccggtgaagt cggccggagt catgagctgt ctggttacga tgtgctggcg gagatcgcgg
     7681 caccccagcg cttcgaggcg ctcgagagtt cgttgctcga ccttgggcgc ctccaccgac
     7741 cagtcgacgg ggtgatccgg gtcctgactg ggcgcgagca ccagaacgaa gagactctca
     7801 cagccgggcg gtgcctgcgc cggatcggtc cgggtcgtcg cgacgacata aaacgacggg
     7861 tccgccggca tccgatgctc gtcgcagagc aaacgacagg actccaagag gtcggacgag
     7921 accaccagat tgtggtggag gaggtcgggg aaagtccggt tcacgccgag acagagcagg
     7981 acgactgagc agctgaactt cttgcggtcg atacgggggt acggctcgcc cagcaggtga
     8041 gtgtaagtat agggcagatc cgcgttggca accacgatat cggctttgat ttcacggccg
     8101 tccgcgaggc gcgccgcggt aacgcgatgc gcctccgtcg ccagacgagt aacgtcggcg
     8161 tcgtagtggt actggactcc caattctttt ccgaccgcct ctaaggcggt cgcgagctgg
     8221 tgcatcccgc caatggggta gtagatgcct ccggcgatct ccgtatgggc caatagtccg
     8281 tagatgcctg gggaggcgta gggcgagagg ccgaggtaga gagtctgaaa cgtcaccgcc
     8341 tggcgcagcc gctcatctcg aaaaaatcga cgtgtcatcg cctgcagatc acctagcgcc
     8401 gctcgcccgt accgcagccc catctggcct agctggagca ccgccgaggg ccggtccatg
     8461 cctcgtcgga tgaaaccgat gccgcgccgg tacaactcgc cggtatgggc cagccattcg
     8521 agtgcgcgcg ggccgcatcc cggctcgatg cgctcgaagt tggcgatcag ctgggggagg
     8581 tcgctggtga tctcgagccg ggagccgtcg gcaaaatgga gccggtaaca cgggtcgcac
     8641 cggatgagcg tgatgtagtc gtccagccga cgatcgactg cagtaaacag ctcatcccag
     8701 tactcgcgca tcatcaccat cgacggccct gtgtcgaagg tgaacccctt ggacttccag
     8761 accccggctc ggccacctgg ctcggcatgg cgttcgacca gagtcacggc gtagcccgcg
     8821 tgcgcaagac ggatcgcggc agccagtccg ccgatgccag cgcccacgac gagcacccgg
     8881 actggtgtgg aggctcggga gtgtggctga ttgccgcgag agagagcgag aacttgcgtc
     8941 atggcaggca acgtctcgcg ggaaggcgtc gcagtcaacc ggcccgccga ttgcctgccg
     9001 cctcatgtgt ctgagccgga ccgctgcctc gcgggttgcg cgagcagaag gcttgcgtgg
     9061 ccgcccgcta ggcagccacg caggggggac gaggtcgtta ctgggttact gtaatggtcc
     9121 caacgaaacc cgggtacaca ctcgggggat ggatcgagca gtggtacggg aacgtgcctg
     9181 tcgtactgaa cgtcatctcg aacgtcccgg cgttcggact cagtactgcg ctgtcccaga
     9241 cccccatgtc gctcgtggtc gtatgtgcgg aaggcccgtt gttggtccag cgcacggtgg
     9301 tgcccgcctt cacagtcacg ctcgcaggcg tgaacgtaaa gtctcgaatg gaaacgtcga
     9361 caaccgaacc gtttgacccg ccgccgccgg taggcggctg aacgaccggt cctgtcgtat
     9421 tgctgctgca cgacaccagc gctgtgacta ataaggcatt ccgaagtgcg attccaagtt
     9481 tcatcgaaaa cctcccgggc aagggggagg tgggcaactg gcaacctgcg gtggtgcaga
     9541 tagcacgatg tccgatcccc tcatgagtgc taaggacgac cgcactcgac tgttcagcca
     9601 cagaaggcgc gcagcccgcg cgaggggaca aaatgcgcgc gcgcgacggg acaaaatgcc
     9661 cagcctgcgg tgtagttcga ttagcgatcg gtgtgggccg tgcggcggcg ggacaacttt
     9721 tcctgctcgt ccaggcgccg accgcgatac acgctgactt ccgagccctc ggccagaaag
     9781 gccgagcggg gaggccccgt caggcactcg gcaaacccgg cgccatagac aaggtggacg
     9841 tcgcagtcga actcggcgct gaccgcctgc cagacccgcc atggtggatg caccacctga
     9901 tattccttgc agcccccgtc gcgctgcgct gtgtagcccc agtagtggtc ggtgacgaat
     9961 tccgcttcgg aactctgctg cggcagagca gcgccgccga cggtccgaac ctcgagactg
    10021 tgccagcgac cgccgagctg ccagaagtac gccgctcgtc cgggagcgcc gtcgcctttg
    10081 ccctccaggg ccagctcatg gcgcatcggc accacggtgt agggctcgtt gtagcaccag
    10141 cgcgcgacta cggcgacggc gcggcggggc accagctcgc ggacaaaaac gacggcgcgc
    10201 cgccagcgct cgtcctctcc acggcggcgc acgtagaaac gcagattcac ctcgtcaaag
    10261 tcgcgatgac cgggcacggc gattccgagc acgcgagtgt cgagaaaccg aaacccgacg
    10321 acgcttgcca gcacggcgcc gtcgaaggtg tcgagctccg tgccggcggg cacgagtggc
    10381 tcgaggagcg accggggcac ctcgtagttg agcatggcga ggtgccgcca cgcggcagtg
    10441 agaaacggtc gttccgtggt catgctgcgt gatcggccgc gttgatcgtg gatgctggtg
    10501 gatcgtcgat gcggccgcga taaagccccg tatgcgaggc cctattcgcc gggttgattc
    10561 cgatggccat gccaccgagg cagacgccgc caggcactgg cgagtacagc cagcaacgca
    10621 gagggcaacc agacccatgg cagttcggtg cgcaaggctg ccaatccgcg cgcgctgaaa
    10681 aagccgcgag gggtgagagg cgacacccga atcgggcgga ttgggaagaa gtagcgagtg
    10741 ttatctatcg gggcaaagaa cgccaccccg cgcccgccgt ccgtgaacgc atcgagaaat
    10801 ccgtgcgacg cggtggcgag aaacaaatag gcccacaacg cggtcgcgcg gagcccagga
    10861 actccgcgcc ggaagccaga aaatgtgacc acggctgcca ggcccgctgc gaaaaggagc
    10921 gaatgcgaca agcctcgatg gccgagcacg tcgccgtaga cgatgccgac gcgaaagcct
    10981 atcacgtcca agtccgggac cgcggcgcag gcggccccta cgacccacac ccgtttcggg
    11041 atttcagggc ggtagaacag cgcgccgatg ccgagtgccg ccaccgcgtg ggaaaaggct
    11101 gagggcatat caacctcggt gaagtggggc tcgtttgact accgccgctc cgcaattgtc
    11161 cgccacttgt ccccctcatg gtaatcgtcg tcccggagcg agcagcgcga actcgaggcg
    11221 cggttggagg tgactgatgg aacgacaaac gcccagccac ctaaattcgg caggtcggcg
    11281 atcatagggt ccttggtggc gtcctgagtc cggcgtcgcg gtggaagcga ggacccgtgg
    11341 ctttgcggcc ccggctttcg ctgggtgcgc ctttgtcgat gggttgcgct ggaatagcga
    11401 taccctacga tccggcattg aggcggtgaa aagcgagcca ctcctgacgc cacttcgcgg
    11461 cgtgttgccg tgttggtaag ccgaccggcc caagttcgcg ctagcgtggc gctgtacccc
    11521 ctaccagatt ggatcgcaca tcggcaagca gcggctccgc cgatgccctc tcgcctgctc
    11581 cagcgacaag tcttgcctgc ggcaaaggag gtcccgatgc ctcgcacttt tccgctcgtg
    11641 ctcgggtgga tggtctccat cgggagcgcc tgtggtggca acgatgccgc ggcgccgacc
    11701 agtggcaccg ccgagcggac gccgcttgcc gaggtccgca ccctctcggg gtcgatcatc
    11761 tgggacgcca agtcgcagat gaagcaggtc gctagcacca aggggtgcga ctcgtggact
    11821 ccgacctggg cgcctgacgg gaacctctat agcggctacg gtgactgcca aatgtcgggg
    11881 gtgccagtca agctgggcat ggggttcgcc agaatttcag ggtccacagc ttacggtgtg
    11941 tcggtcgccg ccgtgccaac tggtgaccca gccaactgga acgacgcagc cactggtgcc
    12001 ggcgtggatg cgaccggtaa cggcacggcc agtgagaagc cggccggcat gctccagatc
    12061 ggcacccggc tctgggcctt gatccggaac atcaattccg gtggccatgg cgtgcggctc
    12121 aaatactccg acaactacaa caccaccagc cctgacttta cctgggtcag ctggacgttc
    12181 accgagctcg gatacgcgtc gttcgtccag tatggcaagg gctacgccgg cgggccgacc
    12241 agctatgtgt acgcggtggt accgatgcgg tcgagctcga ccggaagcgt cagtaacagc
    12301 gcgtacgact cagtgcccgc gttcgggctt ctccgcggtg ctcgcaccga cctcaggatt
    12361 cgcaccaact ggaagtactt ctgcggtccc gcatccacac cctcgtggtg ctccgcttct
    12421 tcgtcggcga agaacgtcta cttcaagtcg ggcacgaagt ttcgtccgcg accaggcatg
    12481 agctggaatc ccggactggg gcgcttcatg ctgacgctgg tttacgacgc gacccctgac
    12541 accagcaaca cccacgacac ccgctttgcc ggctctctca tggtcctcac ggcaccgaac
    12601 ccgtgggggc cgtggacaac tgtgtactcg agtggcagca atccctggcc gggcggcact
    12661 cccaacaccg tcggctgcgg cggctccctc aaccggtggg gcgccggcga ccgggcggac
    12721 atcccgacga agtacatg
//
LOCUS       DASRJQ010000657         2416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3822378, whole genome shotgun sequence.
ACCESSION   DASRJQ010000657 DASRJQ010000000
VERSION     DASRJQ010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3822378"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="VFB89_14935"
     CDS             complement(<1..1033)
                     /locus_tag="VFB89_14935"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZM28641.1"
                     /translation="MPEPLSPNIQHLKGSETVAISNETKRRRAAGEDVLDLSVGEPDF
                     DTPAPAAQAGTQAIQRGQTRYPPNNGLAELRAAIGRRLALLSGGRSVNPDNIVVSSGS
                     KQSIFNACFTLFGPKDQVLIPSPAWVSYPQIVHLCRAEPTLVPGALDWGLKVSVRDLD
                     RASTKRTKGLIICSPCNPTGSVYTAAEIKSIAEWARKHEVWIITDEIYRCINYGTGPA
                     ASFLDLPDDLLERTVVIYGASKAYAMTGWRIGAALAPAHVTKAMAALQSHTTTGANHP
                     AQWAAVTAFSDDRVDAEVQRMTAAFRRRRDYLVARVRAEMPGLEFVEPHGAFYFFFRV
                     DGIRESEPVT"
     gene            1177..>2416
                     /locus_tag="VFB89_14940"
     CDS             1177..>2416
                     /locus_tag="VFB89_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28642.1"
                     /translation="MTELGQLQLADAVAHALTELGWAADDPRVREAAPTAARGRNLAV
                     VTPPSPAFAAPALAGLASRLERHELQGPALLLCPEAELEEWGLLASTLLGGSTVRVEV
                     ARGASRGGKRLQSGNIDLLIASPLSTAVLIARGTLKGERVGAIMLAGPERWPDAEWLV
                     SLMADIPQERQRIVVASAPERAADLVDRYARKALVLGAPADVPAKPLGPVRTVTVAWN
                     RRIGALGELLELLDPGSVVVWTADRSRHGAIRAVLPRDGSVQVMTGDAPRAEIVIAFD
                     PPDAPRLGQLLQAGEVVLLVPPGTESYLARIASPRRPLRLPGPLDAAADSAAQRRSAI
                     SREIEAGRLDQALLTLAPLFERYDATAVAAAVYDLWVGAGSSAAPAVIQVAAASTPAT
                     SKVFVGVGKSDGATPNDLVAV"
BASE COUNT          392 a          790 c          819 g          415 t
ORIGIN      
        1 cggtcaccgg ttcgctctcc cggatgccgt ctacccggaa gaagaagtaa aaggcaccgt
       61 gcggctcgac gaactcgagc cctggcatct cggcacggac ccgcgccacc agataatcgc
      121 gccggcgccg gaaggccgca gtcatccgct gcacctccgc gtccacgcgg tcgtccgaga
      181 acgcggtcac cgcagcccat tgggccggat gattggcccc ggtggtcgtg tgcgactgca
      241 gcgcggccat ggccttggtc acgtgtgccg gggccagcgc cgcgcctatg cgccagccgg
      301 tcatcgcgta tgccttgctc gcgccgtaaa tcacgacggt gcgctcgagg agatcgtcag
      361 gcagatccag gaacgaggct gccgggccgg ttccatagtt gatgcaccgg tagatctcgt
      421 cggtaatgat ccagacctcg tgcttccggg cccactcggc gatcgacttg atctcggccg
      481 cggtatagac cgagcccgtc ggattgcagg gcgagcaaat aataaggccc ttggtccgct
      541 tggtgctggc ccgatcgaga tcccgcacgc tcaccttgag tccccagtcc agcgcgccgg
      601 gaaccagggt aggctcggcc cggcagaggt ggacgatctg ggggtacgac acccaggccg
      661 gtgagggaat gagcacctga tctttcggac cgaacagcgt aaagcaggca ttgaagatgg
      721 actgcttcga gcccgagctt accacgatgt tgtccggatt caccgaacgt ccgccggaca
      781 gcagcgcgag gcggcgtccg atggctgccc gcagttctgc caggccgttg ttgggcgggt
      841 atcgggtctg acccctctgg attgcctgag ttccagcctg tgccgcgggt gccggtgtat
      901 cgaaatccgg ctcacccacg ctcagatcaa gaacgtcttc gcccgctgcc cgccggcgct
      961 tggtctcatt gctgatggcg acggtttcgg agcctttgag gtgctgaata ttgggcgaaa
     1021 gtggctcagg catgcctgtg catccggtgc aaaggtgagc ttggtgagtt gggggaaagg
     1081 ttcgtcggcg atccggctat gtcaacagtt ggcaccgtca acgctggggg cttaggttgc
     1141 cgcccgggac taccagcgat acgaaggcac acgcgcatga ccgagcttgg gcaactacag
     1201 ttagcggatg cagtcgccca cgcgctgacc gagctcggct gggcggcgga cgatccacgc
     1261 gtccgggaag ccgctcccac tgctgcacga ggccgtaacc tcgccgttgt cacaccaccg
     1321 tctccggcct ttgcggcacc cgcacttgcg ggtctggcca gccggctcga gcgccacgaa
     1381 ctccagggcc ccgccctcct gttgtgcccc gaagccgaat tggaagaatg gggactgctc
     1441 gcctctacgc tgctcggcgg cagtacagtc cgggtcgagg tcgcgcgcgg agccagccga
     1501 ggcggcaagc ggctccagag cgggaacatc gacctgctga tcgcgtctcc gctctccacg
     1561 gctgtgctga tcgcgcgcgg cacactcaaa ggggagcgcg tcggcgccat catgctcgcg
     1621 ggaccggaac gctggcccga tgcggaatgg ctcgtctcgc tcatggccga catccctcag
     1681 gaacgccagc ggatcgtcgt ggccagcgcg ccggagcgcg cggcagatct ggtcgatcgt
     1741 tatgcccgga aggcgctggt tcttggcgca cccgcggacg ttccagccaa gccgttagga
     1801 ccggtgcgca ccgtgacggt ggcctggaat cggcggatcg gagcattggg cgagttgctc
     1861 gagctgctcg atccaggctc ggtcgtggtg tggactgcgg atcggagccg gcatggagcg
     1921 atccgggcag tgcttccccg cgatgggtcg gtccaggtga tgaccggcga cgctccgcgc
     1981 gccgaaatcg taatcgcctt cgaccctccc gatgccccca ggctggggca gctgctgcag
     2041 gcaggcgaag tcgtgctgct ggtaccacca ggaaccgaat cgtacctggc ccgcatcgcg
     2101 tctccgcgcc gcccgctgcg gcttcccggg ccgctcgatg ccgcagccga ctcggccgcg
     2161 cagcgtcgca gcgcgatctc gcgggagatc gaggcgggtc ggctcgacca ggccctcctc
     2221 acgctggctc cgcttttcga gcgatacgac gcgactgctg tggcggcagc ggtttatgat
     2281 ctatgggtag gggccggcag ctcggcagca ccggcagtga tccaggtagc tgccgcgtct
     2341 acgcccgcca cttccaaggt gttcgtgggc gtgggaaaga gtgacggcgc aacgccgaac
     2401 gacctcgtcg ccgtgc
//
LOCUS       DASRJQ010000658         4727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2372473, whole genome shotgun sequence.
ACCESSION   DASRJQ010000658 DASRJQ010000000
VERSION     DASRJQ010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2372473"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..664)
                     /locus_tag="VFB89_14945"
     CDS             complement(161..664)
                     /locus_tag="VFB89_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28643.1"
                     /translation="MNHAQCIPTFPLTNGQPGAAVVPYHTIAFSQQKLRAALRRSGAQ
                     EPALTYGFVVHSRRQQERPTLGLITLSGESLPLNRRLLTALDGLPLWLFGHARITLDQ
                     GIPMDGIPEEKLDDAPLTGLLMHIATFDVTAVAQNLVQVEAAIRADTLVQPLIVLTTA
                     RPGAWPR"
     gene            754..2763
                     /locus_tag="VFB89_14950"
     CDS             754..2763
                     /locus_tag="VFB89_14950"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM28644.1"
                     /translation="MFCSRCGSEISDNSKFCPSCGLDLTATTPVRSMASGAVADLELV
                     REALNSEYEVFEELGRGGMALVYRAKDRHLEREVAVKVLPFTLAFDAEFVERFQREAR
                     TAAQLEHPNIIPIYRVGRSGRVIYFVMKYLRGGSLATVLGERKKLTPPEIRRLLIEAG
                     SALGYAAQRGIVHRDIKPDNIMFDEFGQCVLTDFGIAKAASGGRLTGTGMSIGTPHYM
                     SPEQARAQPIDGRSDIYSLGVVAYQCLAGVVPYDGEDSFSIGYKHITEPIPSPHLPTA
                     DERRVFEVIKRMLMKEPADRFQACEELIASIQGQPTANAGAFKGSQPGRSTGTPAIAS
                     QPTTPINSPSVERRARPRPERGIADRSPVMRQSSGSPFLWILLLVGVLGAGGVFYYRS
                     MSGGAAPAAAASKDSTADSTRSTTLGADSARSDTTKNIPPTPPPPSPAAPVPQPATAT
                     SPKPEPSAVSGTPAPRSPKADSAARPAVPPAAPADSGGIRVTNLPRGSSVLIDEQPVT
                     EPETRLPAGRHVLAISAPQHNFFTDTIVVQPGKVTEVTPQLTRIGAPVAAKGRDSTRP
                     ASTCSPGPRYNADGSCFDERPKPVSPPFVPVPADAEETPRPSLLWVKVSAEGKTLEVR
                     SLKPSSDAAFERDVRAFAQELPWYPASKGGQPVEAWTQMIFRPQP"
     gene            2868..3368
                     /locus_tag="VFB89_14955"
     CDS             2868..3368
                     /locus_tag="VFB89_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HZM28645.1"
                     /translation="MIHPTAFIAPTASVMGSVNLGPESSVWYQAVLRGDLAQITLGAQ
                     SNIQDGAVVHVDEGVPCTVGQRVGVGHRVILHGCLIEDECLIGMGSVLLNGVRIGSGS
                     VVAAGAVLPEGMVVPPGSVVMGVPGRIIRPVDETLKRRIAGTWAHYVELARQHRAGRY
                     PLERGQ"
     gene            3607..>4727
                     /locus_tag="VFB89_14960"
     CDS             3607..>4727
                     /locus_tag="VFB89_14960"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HZM28646.1"
                     /translation="MSPSTRRDAVTLSPNAITVLEKRYLIKDDAGKPVETPSDLFWRV
                     ARTIAEPDRRYGASGGAVEALAEAFYGLLADRFFMPNSPTLMNAGRPLGQLSACFVLP
                     VDDAISNGRSGIYDTLRAMALVHQSGGGTGFSFSRLRPKNDIVRSTMGVASGPVSFMS
                     LYDASTDVVKQGGTRRGANMGILRVDHPDILEFISCKDDITKITNFNISVAVTDAFMR
                     AVEAGGMYDLLHPKTQQPVGQLEARDVWERIIHGAWKTGEPGVFFIDRANYYNPVPHL
                     GAYEATNPCGEQPLLPYDVCNLGSINVGAFVSNGQIHWDQLRRVVHLSTHFLENVIDA
                     NQYPLQEITELAQRIRRIGLGVMGLADLFVRVGVAYDSE"
BASE COUNT          826 a         1490 c         1546 g          865 t
ORIGIN      
        1 accaggaggc ggcgctgcgg accaaggagg ggcaggagct gtatgcgaag ggggtctatg
       61 aagggatcgt ggagttcctg aagagcgtga acggtaaacg gtgaacgggg actccgatgt
      121 tcaccgaagc ccccgttcac ctttaccgtt tacgcagtct ctatctgggc catgcccccg
      181 gccgggctgt cgtcaggacg ataagcggct ggaccagtgt gtcggcccgg atggcggctt
      241 cgacttgcac cagattctga gcgactgcag tgacgtcgaa cgtggcaata tgcatgagca
      301 ggccggtcag cggcgcgtcg tccaacttct cctccggaat gccgtccatc ggaatgcctt
      361 gatccagcgt gatccgcgcg tggccgaaca gccagagagg cagcccgtcc agcgcggtca
      421 acaggcgccg attcagggga agactttctc cgctcagcgt aatgagtccc agagtggggc
      481 gctcctgttg gcgacgggaa tgcacgacga agccgtacgt gagagccggc tcctgtgcgc
      541 ccgagcgtcg aagcgccgcg cgcagcttct gctggctgaa ggcaatcgtg tggtacggga
      601 ccacagcagc tccgggctga ccgtttgtca gggggaacgt cggaatgcat tgagcatggt
      661 tcatgccatg gggtaccgga gactgtttgt caggccttcc gatggcacgc gtactgccat
      721 tgactccccc ctctccctag gtagcttgcg cccatgttct gctcccgctg cggaagcgag
      781 atcagcgaca actccaagtt ctgcccttcg tgcggtctcg atctcaccgc taccacaccc
      841 gttcgatcga tggcatcggg ggccgtggcg gatctcgagc tggttcgcga agccctcaac
      901 agcgaatacg aagtgttcga ggagctgggg cggggcggca tggcgctggt ctatcgggcc
      961 aaggaccgtc acctcgagcg tgaggtcgcg gtcaaggtcc tgccgtttac gctggcgttc
     1021 gacgctgagt tcgtcgagcg attccagcgt gaggcgcgca ccgcggcgca gctggagcat
     1081 cccaacatca ttccgattta tcgggtcggc cgctcgggcc gggtgatcta cttcgtgatg
     1141 aagtacctcc gcggcgggtc gctcgcgacg gtgctagggg agcggaagaa actcacacca
     1201 cccgagatcc ggcgcctgct gatcgaggcg ggcagcgcgt tggggtacgc ggcgcagcgc
     1261 gggatcgtgc accgcgacat caagcccgac aacatcatgt tcgacgagtt cgggcagtgc
     1321 gtcctcacgg acttcggcat cgccaaggcg gcgtcgggag gtcggctgac cggcacgggc
     1381 atgtcgatcg gaacgcccca ctatatgagc cccgagcagg ctcgagccca gccgatcgat
     1441 ggccggagcg acatctacag cctcggcgtc gtcgcctacc agtgtctcgc gggcgtggtc
     1501 ccgtacgacg gagaggacag cttttcgatc ggctataaac acatcaccga gccaatcccg
     1561 tcgcctcacc tccctaccgc cgatgagagg cgcgtgttcg aggtgatcaa gcggatgctg
     1621 atgaaggagc cggccgaccg attccaggcc tgcgaggagc tgatcgcctc tatccagggg
     1681 caaccgaccg ccaatgcggg tgccttcaag gggtcgcaac ccggccgttc gacggggacg
     1741 ccggccatcg cgagccagcc gacgacgccg attaattctc cgtcggtaga gcggagagcg
     1801 cggccacggc ccgaacgcgg cattgccgac cggtcaccgg tcatgcggca aagctcgggc
     1861 agtccgttcc tctggattct tctcctcgtg ggcgttctcg gagccggcgg cgtcttctat
     1921 tacaggtcga tgagcggagg cgcggcgccg gccgccgcag cgagcaagga ctcgacggcg
     1981 gactccacgc gctcgactac gcttggcgcc gacagtgcgc ggagcgacac gaccaagaat
     2041 attcccccga ctccgccgcc gccgtcgccg gcagcgccgg taccgcagcc cgcgacggct
     2101 acctcaccca agcctgaacc atcagccgta tccggcacac ctgctccccg ttctcccaaa
     2161 gccgatagtg cggcccgtcc ggcggttccg ccggctgcac cggccgacag cggtggcatc
     2221 cgggtcacga atctgcctcg gggatcgagc gtcctgatcg acgagcagcc ggtgaccgaa
     2281 cccgagacgc ggcttcctgc cggccgacat gttctcgcga tctcggcgcc ccagcacaat
     2341 ttcttcaccg acaccattgt agtgcagccg ggtaaggtta ccgaggtcac gccccagctc
     2401 acccggatcg gcgctcctgt cgcagccaaa ggccgggata gtactcgacc cgccagcact
     2461 tgctcgcctg gtccccgcta caacgccgac ggctcctgct tcgacgagcg cccgaagccg
     2521 gtatcgccgc cgtttgttcc agtcccggcc gacgcagagg aaactccccg tccctcgttg
     2581 ctgtgggtca aagtttccgc cgagggaaag accttggagg tcaggtcgct caagccgtcc
     2641 agcgacgccg ccttcgagcg cgacgtccgc gccttcgccc aggagcttcc gtggtatccg
     2701 gcgagcaagg gcgggcagcc ggtggaagcc tggacccaga tgatcttcag gccgcagccg
     2761 tagggagcta tcagctatca gctatcagct agttccgctg agtgctgaat ccctatttct
     2821 gtcgccaacc ctctgctagc tgatagctga tagctgatag ctccctcatg atccacccca
     2881 ccgccttcat cgcccctacc gcctccgtca tgggctcggt gaatctcggg ccggagtcga
     2941 gcgtgtggta tcaagcggtt ctgcgagggg atctggccca gataacgctc ggtgcgcaat
     3001 ccaacatcca ggatggagcg gtcgtccacg tggacgaggg agtgccgtgc acggttgggc
     3061 agcgagtggg agtaggccac cgggtcatcc ttcacgggtg cctgatcgag gacgaatgtc
     3121 tcatcggaat gggtagtgtg ctgctcaacg gggttcgcat cggtagcggg agtgtcgtcg
     3181 cagcgggggc agttcttccg gaagggatgg tagtccctcc agggtcagtg gtcatgggcg
     3241 tgccgggacg gatcattcgt ccggtcgatg agacactcaa gcgacggatt gcggggacct
     3301 gggcgcatta cgtggagctg gctcggcagc atcgggcggg gcgctatccg ctcgagcggg
     3361 gccagtgatg cgacggtgcg gcatctctgt agcgcgtgag gcgatttggt cggttttcca
     3421 cataggagga tgtgattatt tttgtgatgt gaacgaaggg atttcgcgtc gtgacgcgga
     3481 ttttacgagg cattgtgcga ccatgtgggt tggcctagct tggccctccg tttcggcgaa
     3541 tcccccaacc gccgaagcag ccgggttggc acgcacgcag gaatccttca cagagcgggg
     3601 ttgcccatga gtccgtccac acgacgggac gccgtcacgt tgtcgcccaa cgccatcacg
     3661 gtgctcgaga agcgctacct catcaaggac gatgcgggta agcccgtcga gacacccagc
     3721 gatctgttct ggcgcgtcgc ccgaacgatc gcggagcctg atcgtcgcta cggcgcgtcg
     3781 ggtggggcgg tggaggcgct ggcggaggcg ttctatgggc tcctggccga ccggttcttc
     3841 atgcccaact ctcccacgct catgaacgcg ggccggccgc tggggcagct ttccgcctgc
     3901 ttcgtgcttc cggtggacga tgcgatttcg aatggacgga gcggcatcta cgacacgctc
     3961 cgggcaatgg cgttggtgca ccagtctggc ggtggcaccg gcttcagctt ctcgcggctc
     4021 cggcccaaga acgacatcgt ccgctcgacc atgggtgtcg cgtctggtcc ggtgtcgttc
     4081 atgtcgctgt acgacgcatc gaccgacgtc gtgaagcagg gcggaacccg gcgcggcgcc
     4141 aacatgggga ttctgcgggt ggaccatccg gacattctgg agttcatctc ctgcaaggac
     4201 gacatcacta agatcaccaa cttcaacatc tcggtggcgg tcaccgacgc gttcatgcgc
     4261 gcggtcgagg ctggcgggat gtacgacctg ctccatccca agacccagca gccagtcgga
     4321 cagctcgaag ctcgcgacgt gtgggagcgg atcatccacg gtgcctggaa gaccggtgaa
     4381 ccgggggtgt tcttcatcga tcgcgccaat tactacaatc cggtcccgca cctcggcgcc
     4441 tacgaggcga ccaatccgtg tggcgagcag ccgctcctgc cgtacgacgt ctgcaacctg
     4501 ggctcgatca acgtcggcgc gttcgtcagt aatgggcaga tccactggga tcagcttcgt
     4561 cgagtggttc acctctcgac ccatttcctc gagaacgtca tcgatgccaa ccagtacccg
     4621 ttgcaagaga tcaccgagct tgcgcagcgg atccggcgca tcggcctcgg ggtcatgggg
     4681 ctggccgacc tgttcgtgcg ggtgggtgtg gcgtacgact cggagga
//
LOCUS       DASRJQ010000659         5796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1613994, whole genome shotgun sequence.
ACCESSION   DASRJQ010000659 DASRJQ010000000
VERSION     DASRJQ010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5796
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1613994"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VFB89_14965"
     CDS             <1..709
                     /locus_tag="VFB89_14965"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZM28647.1"
                     /translation="RTDRGDAGQRVVVPFLARQGAPAVSALIISHAHADHVGGAPAVL
                     DRFRTGIVIEPGRPFADPAYYRFLDEVAADGVPWHPGVPGDRFDLDGISFSLLHPDRT
                     WRGLGDDLNEDSVILLVEYGGFRALFPGDAGLLAEAWLEGRVGHISLLKIGHHGSRGA
                     TGNDWLDELVPRVAVISVGRNNYGHPSPQTLARLAAHRVDLWRTDRDGQVDVTTDGET
                     MTVRSRGRTETLELRKR"
     gene            798..1844
                     /gene="fbp"
                     /locus_tag="VFB89_14970"
     CDS             798..1844
                     /gene="fbp"
                     /locus_tag="VFB89_14970"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HZM28648.1"
                     /translation="MIPNPTSVTTIEAFILEQESRYPEATGELSNLLYDVALAAKIIA
                     AAIRRAGLVNILGSVGHRNVQGEEQQKLDVFANETIKNCLKHTGRVCVMASEEDEDII
                     PIPPEFPLGKYAVLFDPLDGSSNIDVNAAVGTIFSIYRRVSLDVRGTVSDVLQPGCKQ
                     VAAGYVMYGSSTMLVYTTGEGAHGFTLDPTIGEFLLSHPRIITPRVGTYYSVNESNFG
                     RWDRGFQTAVRGLKGDMPDVMKAKNCRYAGSLVADFHRNLIAGGIFLYPGDTKNPNGK
                     LRLLYEASPMAFIAEQAEGSATDGVNRILDVMPDTLHQRTPLVIGSREDVGFVADTLR
                     RAASAPQPVSTSAL"
     gene            1841..3379
                     /locus_tag="VFB89_14975"
     CDS             1841..3379
                     /locus_tag="VFB89_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HZM28649.1"
                     /translation="MKQVYGPGDWTGKPEHDLGEPGAYPFTRGVYSSMYTSRLWTMRQ
                     YAGFGTAEETNERFRKLLAAGQMGLSVAFDLPTQMGYDSDHPMAAGEVGRVGVAIDTV
                     EDLATLFREIPLDRVSSSMTINATAAILLAMYIVVGEEQGVPRSKLTGTVQNDVLKEY
                     VARGTYIYPAKPSLRLVADIFRFVADEKLSFNPISISGYHMREAGATAVQEVGFTLAN
                     ALEYVKVAVAAGLDVNAFGPRLSFFFASHNDVFEEAAKFRAARRLWARLVRERFGADD
                     ATCRLRFHTQTGGVTLQAQQPMNNVVRVAYQALAAVLGGTQSLHTNGYDEALALPTEE
                     SATLALRTQQVLGYESGVARVVDPLAGSWYVEALTDEIEQEARALVEQVDELGGAAAA
                     IERGFLQETIARSAYALQRAQESGEMTVVGVNRFTDDSAPPAIATPNFPQLEQRQRAR
                     LAAVKAKRDRLAADSALSAVGRAARSDEPLMPTIIAAVRARATLGEISDALRQAWGVY
                     RPPV"
     gene            complement(3403..3741)
                     /locus_tag="VFB89_14980"
     CDS             complement(3403..3741)
                     /locus_tag="VFB89_14980"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZM28650.1"
                     /translation="MDLGATAKALREEPHPAKDGHRQIGLLHQEHIRVVLFAFDRGGT
                     LPKHQAPGLVSIQVLSGRLKVTTPATTHELGTGHLLVLEPDMPHDVEAPEASDMLLTV
                     HLDGGRSGAP"
     gene            3913..4230
                     /locus_tag="VFB89_14985"
     CDS             3913..4230
                     /locus_tag="VFB89_14985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZM28651.1"
                     /translation="MPTYEYECPSGHVFDKFQKMTDKPRAKCPVCGKVATRKISGGAG
                     LVFRGSGFYITDYGKDGKGPRKAETEKSGESAPAKSDAKEASPAKADKSAEAQPKSKK
                     AAE"
     gene            4227..>5796
                     /gene="argS"
                     /locus_tag="VFB89_14990"
     CDS             4227..>5796
                     /gene="argS"
                     /locus_tag="VFB89_14990"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HZM28652.1"
                     /translation="MSQVLKTELARVAERLGHPGIDFVLERPRDSGHGDVATNLGMVL
                     AKLERSKPRQMAERVVAELHLPEGFVSKIEIAGPGFINFWLADDQLADVVGEILERGP
                     AYGQSQIGVGLRVNVEFVSANPTGPLHVGHGRGAAYGDALASLLEWTGHAVTREFYIN
                     DAGVQIDKLAWSLWARVQEAMGRDSDVPDGGYHGLYLAEAAEQVLREKGADFADLPAE
                     EGVRQCRALALRMQREEQDRDLADFGVRFNVFTSEQSLYDRGMVEETLVLLGEKGLSY
                     EREGALWLRTTQFGDDKDRVLRKSDASLTYLVPDIAYHVDKRSRGFDRVIDVWGADHH
                     GYVPRMRSVLSALGYPPEFFDVALLQLVKVVRGGEEVKMSKRSGEFVTLRDLIDEVGS
                     DAARYFFLMRPGDSQLVFDVELAKRQTDENPVFYVQMAHARLSGIFRTAGKAPEQFNG
                     RLDVAALPADEDQELLKQLVIFRETVEKAAQERAPHRITNYLQQLATSVHGWYHHTRA
                     VGAPEGPATEHGRLL"
BASE COUNT         1071 a         1818 c         1883 g         1024 t
ORIGIN      
        1 caggacggat cgcggggatg cggggcagcg ggtcgtggtg ccgttcctcg cccgacaggg
       61 ggcgccggcc gtctcggcgt tgattatctc ccacgcccac gccgaccacg tcggcggcgc
      121 gcctgccgtc ctcgatcggt ttcgcaccgg catagtgatc gagccgggca ggccgttcgc
      181 agatcccgcc tattatcgct ttctcgacga agtggctgcg gacggcgtgc cttggcatcc
      241 cggtgtgccg ggcgatcgat tcgacctcga cggcatctcg ttcagcctgc tgcatccgga
      301 tcgtacctgg cgcggactgg gcgacgatct gaacgaagac tcggtgattc tgctggtcga
      361 atatggcggg ttccgtgctc tgttccccgg tgatgccggc ctgctggccg aggcgtggct
      421 cgagggccgg gttgggcaca tctcgctgct caagatcggc catcacggaa gccgcggcgc
      481 caccggcaac gattggctcg acgagctggt gccgcgggtt gcggtaatct cggttggccg
      541 caacaactac ggtcacccat cgccgcaaac cttggccagg ctggcggcgc accgggtcga
      601 tctgtggcga actgatcggg atgggcaggt ggatgtgacg acggacggag agacgatgac
      661 ggtaagaagt agaggccgga cggagacgct tgaactacgt aaacggtgaa cggtaaacgg
      721 taaacgggaa gtccgtcaca ctacgaaact cccgttcact gttcaccgtt cgcgcttttc
      781 ctgaacggag actagccgtg atacctaacc ccacatccgt caccaccatc gaggccttca
      841 ttctcgagca ggagagccgg tatccggaag cgaccggcga gctctccaat ctcctctacg
      901 acgtggcact cgcggccaag atcatcgcgg ccgcgattcg gcgggcgggg ctggtcaaca
      961 ttctcggtag cgtcgggcat cggaacgtgc agggtgaaga acagcagaag ctcgatgtct
     1021 ttgccaacga gaccatcaag aattgcctga agcacaccgg ccgggtctgt gtaatggcgt
     1081 cggaggaaga cgaagacatc attccgatcc cgcccgaatt cccgctgggc aaatacgctg
     1141 tccttttcga cccgctcgac gggtcttcga acatcgacgt gaacgcggcg gtcggcacca
     1201 tcttcagcat ctaccgccgc gtcagcctgg atgtgcgggg gaccgtgagc gatgtgctcc
     1261 agccgggttg caagcaggtc gccgcaggtt acgtgatgta cggctcgagc accatgctgg
     1321 tgtacaccac gggcgaaggc gcccacgggt ttaccctcga tcccaccatc ggtgaattcc
     1381 tgctctctca cccccgaatc atcacgccca gggtcgggac gtactacagt gtgaatgaat
     1441 ccaacttcgg acgttgggat cggggctttc aaaccgccgt ccggggactc aagggcgaca
     1501 tgccggacgt catgaaagcc aagaactgtc gctacgccgg ttcccttgtt gccgatttcc
     1561 accgcaatct catcgccgga ggcatcttcc tctatcccgg cgataccaag aaccccaacg
     1621 gcaagctccg gctcctgtac gaagcgagcc ccatggcttt catcgcggag caggccgaag
     1681 ggtccgcgac cgacggcgtg aaccggatcc tcgacgtcat gccggacacc cttcaccaac
     1741 gcacgccgct cgtcatcggg tcccgggaag atgttggctt cgttgccgac acgttgcgcc
     1801 gagcagcctc ggcccctcag ccggtctcga cctccgccct gtgaagcagg tttacggccc
     1861 cggggactgg accgggaagc cggagcacga tctgggtgag ccgggagcgt acccgttcac
     1921 ccgcggtgtc tactcctcga tgtataccag ccggctctgg accatgcggc agtacgccgg
     1981 cttcggcacc gcggaggaga ctaacgagcg cttccggaaa ctgcttgccg cgggtcagat
     2041 gggactctcg gtggcgttcg atctccctac ccagatgggc tacgactccg atcatcccat
     2101 ggctgccggg gaggtgggcc gggtaggggt ggcgatcgat acggtggaag acctcgccac
     2161 tcttttccgc gaaatccctc tcgatcgagt ttccagctcg atgacgatca atgccaccgc
     2221 cgcgatcctt ctcgccatgt acatcgttgt cggcgaggaa cagggcgtgc cgcgcagcaa
     2281 gctcaccggg acggtgcaga acgacgtgct caaggagtac gtcgcccgcg gcacctacat
     2341 ctacccggcc aagccctcgc ttcgactggt ggccgacatc ttccgcttcg tggccgatga
     2401 gaagctcagc ttcaatccga tttccatcag cggctaccat atgcgggaag ccggcgccac
     2461 cgccgtgcag gaagtcgggt tcaccctggc caacgccctc gagtacgtga aggtggcagt
     2521 cgcggcgggc ctcgatgtca atgcgttcgg cccccggctc tccttctttt ttgccagcca
     2581 caacgacgtg ttcgaggaag ccgccaagtt ccgggccgcg agaaggctgt gggctcgcct
     2641 ggtccgcgag cgtttcggtg cggacgatgc aacctgccgg cttcggttcc acacccagac
     2701 cggaggcgtg acgctgcagg cccagcagcc gatgaataac gtggttcgag tggcctacca
     2761 ggcgctggct gccgtgctgg gcggcaccca atcgcttcac accaacggat acgacgaagc
     2821 tctggctctt cccaccgaag agtcggccac cctggcgcta cggactcagc aggtcctggg
     2881 ctatgagagc ggagttgccc gcgtggtgga tccgctcgca ggaagctggt acgtcgaagc
     2941 cttgaccgac gagatcgagc aggaagcgcg tgccttggtc gagcaagtgg atgagctcgg
     3001 tggagccgca gcggcaatcg agaggggttt cctccaggaa acgatcgccc ggagcgcgta
     3061 cgctctgcag cgggctcagg agtccggcga gatgaccgtc gttggagtga atcgcttcac
     3121 cgatgattcg gcgccacccg ccatcgccac gcccaatttt ccacagttgg aacagcggca
     3181 acgagcgcgc ctggcggcag tgaaagcaaa gcgcgatcgt ctcgcagcgg acagcgccct
     3241 gtccgcggtg ggccgcgcgg cccgatccga cgagccgctg atgccgacca tcatcgcggc
     3301 ggtccgcgct cgggcgacgc tgggagagat cagcgatgcg ctgcggcagg cgtggggagt
     3361 ctaccgtcca cctgtctgag cggctttctc tcggtcaccc catcaaggcg ccccgctgcg
     3421 ccccccatcc aaatgcaccg tgaggagcat atcgctcgct tccggcgctt cgacatcgtg
     3481 cggcatgtcc ggttcgagca caagcaggtg accggtgcca agctcgtgag tggtagcggg
     3541 ggtggtgacc ttcagacggc cggacaggac ctggatgctc accagaccgg gcgcttgatg
     3601 cttgggcagc gttccgcctc gatcgaacgc gaagagcaca acccgaatgt gctcctggtg
     3661 gagtagtccg atctggcgat ggccgtcctt ggcgggatgc ggctcttccc gcagcgcctt
     3721 ggcggtggcg cccaggtcca ttgcgatttc agggccccga gcacgagtgg aaggcggcgg
     3781 acgaaggcgt tcgtctggag acaccggagc actcacggga gcgggaaggt ttctaagcta
     3841 gcggcgggtt gaaccactgg ccagcggcag ctaagtttat cgcctggttt cagttagacg
     3901 acacgagcga ccatgccgac ctatgagtac gagtgccctt cggggcacgt gttcgacaag
     3961 ttccagaaga tgaccgacaa gccgcgggcc aagtgcccgg tttgcggcaa agttgccacg
     4021 cggaaaatct cgggcggtgc cggtttggtc ttccggggta gcggtttcta catcaccgat
     4081 tacggcaagg acggcaaagg cccccgcaaa gctgagacgg agaagagcgg cgaaagtgcg
     4141 cctgccaaga gcgacgctaa ggaggcgtcg cctgccaaag ccgacaagtc cgccgaagcc
     4201 cagccaaagt ccaagaaggc tgctgagtga gtcaggtgct caagaccgag cttgcgaggg
     4261 ttgctgagcg gctgggccat ccgggcatcg acttcgtcct cgaacgccct cgcgattcgg
     4321 gacacggtga cgtggcgacc aatctcggca tggtcctcgc caaactggaa cggagcaagc
     4381 ctcgccagat ggccgagcgg gtcgtcgctg agctccacct tcctgagggg ttcgtttcga
     4441 agatagagat tgccgggccg ggcttcatca acttctggct ggccgatgac cagcttgccg
     4501 acgttgtcgg agagattctc gagcgcgggc cggcctacgg gcaaagccag atcggagtcg
     4561 gcctccgggt caacgtggag ttcgtctcgg ccaaccccac cggtccgctc catgttggcc
     4621 acggccgggg tgccgcgtac ggcgatgccc tcgcctcact gctcgagtgg accggccatg
     4681 cagtcacccg cgagttctac atcaacgacg ccggtgttca gatcgacaag ctcgcctggt
     4741 cgctctgggc ccgagtccag gaagcaatgg ggcgggacag cgatgtcccg gacggtggat
     4801 accacggtct ctacctggcc gaagcggcgg aacaggttct ccgcgaaaaa ggcgccgact
     4861 tcgcggactt gccggctgaa gagggtgtgc gacagtgccg tgcgctggcg cttcggatgc
     4921 agcgtgaaga gcaggatcgc gacctggccg acttcggggt ccgcttcaac gtcttcacct
     4981 cagagcagtc cctatatgac aggggcatgg tcgaagagac gctcgtgctg ctcggagaga
     5041 aagggctcag ctacgagcgc gagggcgcac tctggcttcg gacgacgcaa ttcggcgacg
     5101 acaaggaccg agtgttgcgc aagagcgatg cgtctctgac gtacctcgtt ccggacatcg
     5161 cgtaccacgt ggacaagcgg agccgagggt tcgaccgggt catcgacgtc tggggcgcgg
     5221 accaccacgg ctacgtgcct cggatgcgct cggttctctc ggccctcggc tatcccccgg
     5281 agttcttcga cgtcgccttg ctccagctgg tcaaggtcgt gcgcggcggt gaagaggtga
     5341 agatgtcgaa gcgctccggc gagttcgtaa ctctgcgtga tctgatcgac gaagtcggca
     5401 gcgacgctgc gcgctacttc tttctcatgc gtccgggaga ctcgcagctg gtgttcgatg
     5461 tcgagctggc caaacgacag accgacgaga acccggtctt ctacgtgcag atggcgcatg
     5521 cccggttgag cgggattttc cgaaccgcag gaaaggcgcc cgagcaattc aacggccggc
     5581 tcgacgtggc agcgctcccg gccgatgagg atcaggagct gctcaaacag ctggtgatct
     5641 tccgggaaac ggtggaaaag gccgcgcagg aacgcgcgcc gcaccgaatc accaattatc
     5701 tccagcagct cgcgacttcg gtgcacggct ggtatcacca cacccgggcg gtcggcgctc
     5761 ccgaaggacc ggccaccgag cacggtcgcc tcttac
//
LOCUS       DASRJQ010000660         3058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_615243, whole genome shotgun sequence.
ACCESSION   DASRJQ010000660 DASRJQ010000000
VERSION     DASRJQ010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615243"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VFB89_14995"
     CDS             <1..452
                     /locus_tag="VFB89_14995"
                     /inference="COORDINATES: protein motif:HMM:NF027875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HZM28653.1"
                     /translation="LAIVAGGGSFPDDFAQGFPGGKYISFSLRIATRRPPAPSGVAVQ
                     EYRLLRPLARPIVPSFKLTTMTAPARLVRVRAPNAMSVEVAGDFTDWEPVALRRGADG
                     DWTAVIVIPKGTHRMNLRVDGGAWGVPPGIPALNDDFGGVVGVLVVE"
     gene            730..2205
                     /locus_tag="VFB89_15000"
     CDS             730..2205
                     /locus_tag="VFB89_15000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HZM28654.1"
                     /translation="MCRLRCNLLAFVAISVASVATLSAQGDSLAETAVEGRGLVSPAV
                     LSALRTTIAERYVTPIPAESLARFKTAEALLESLGDRHTVLFSAEAFRDFQVAAGQHF
                     GGIGARLGVRRDTVYLASLLPNGPAAKAGLVAFDRLVSVNGSSLVGLPADSAVQLIRG
                     PNGSTAKLTVVTAGHKRVVSLVRDAVAVPSVGGVALLDDKIGVVRLRQFGEGATGEVV
                     NAIDALVSSGARGIVLDLRGNPGGLLQEGLSIAQLFLPSGSGLVEVRPRSGLPAQRAR
                     AEAPPRYPNLPLAVLIDGLSASASEILAGALQDAHRAVVLGRQSYGKGSVQETVPLPE
                     GWVVKLTVARWYTPSGRGIDRGPQPADALIDPDVRHPGGIAPDTPLAPDTTVAPPRQA
                     IATLKKSTWARVSDRLADWTEAHRADSTAAPTRETADTLLSGLALPASIEPALADWLG
                     RALTQELLTARYGDTAGEAWSLTHDAEVEAARAWLERRLAA"
     gene            complement(2224..>3058)
                     /locus_tag="VFB89_15005"
     CDS             complement(2224..>3058)
                     /locus_tag="VFB89_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28655.1"
                     /translation="AIWQTDGLVNGLHLHVAVALERMRMGNAWTLKHSTGDVLFGGSG
                     HRLFPATARQPAGGKDWIAVRDEFLSQRAHDNPFFSTTEVAEMSRGDCDRYLGGPSVD
                     CFILGMGQRRWTLTGPLAMMGFCEVVNPGASLPILQLMLGGLDDRQRSGHRFYARFLC
                     EAYPRFFRTVPWQKTGEGLAEPALVSAARRLAKRAGFRVGISRARLNFADYDRLAATA
                     RVAERMAATDLVADEVMDGALRAALQRRHETPIKTRTLLAMLTLETYLRRAAGVVSLG
                     D"
BASE COUNT          523 a          955 c         1020 g          560 t
ORIGIN      
        1 acctggcgat cgtggccggc ggcggcagct ttcccgacga cttcgcacag ggcttcccag
       61 gcggcaagta tatttccttt tcccttcgga tcgcgacgcg gcgacccccg gcaccgagtg
      121 gagtcgccgt gcaggagtac cggctgttac gcccgcttgc ccggccgatc gttccgagct
      181 tcaaactcac cacaatgacc gctcccgccc gactggtgcg cgttcgcgcg cctaatgcga
      241 tgtcggtcga ggtggcgggt gacttcacag attgggagcc ggtggctctc agacggggag
      301 cggacggaga ctggacggcc gtaatagtga ttccgaaggg gacgcatcgc atgaacttgc
      361 gagtcgacgg cggcgcctgg ggcgtgcctc cggggattcc ggcgctgaac gatgatttcg
      421 gcggggtggt tggcgtactg gtcgtggagt gagctcacgc gaccggcgca atgacgaact
      481 caacagcagc tcattcccca actgggacaa gtgatcgtcc tcactacgtg atccagcgcg
      541 ccgccgcgac gggggccgcc tgaacactgt gagccggtaa ctagtgaacc aggggccggc
      601 cttactagtg tgagttgacg tcgctgctgc gggctcgggt gcatgacggc ctgctttctg
      661 ctacggcagc aggccaaagg gcgagttctg gtcgcctcga tgagtgagag agcgagagag
      721 ggaatgtcga tgtgccgact cagatgcaac ttgctggcct ttgtggctat ttcggtggcc
      781 agcgtggcca ctttgtccgc ccagggagac tcacttgccg agacggccgt cgagggccgg
      841 ggcctcgtat cgcctgcggt gctgagcgcg ttgcgcacaa ccatcgcgga gcgatacgta
      901 acccccattc ccgctgaatc gctggcacga ttcaagaccg ctgaagcgct gctggaatca
      961 ttgggtgacc gccacactgt gctgttctcg gcggaagcgt tcagggattt tcaggtggcg
     1021 gccggccagc atttcggcgg catcggagcc cggctggggg ttcggcgcga tacggtgtat
     1081 ctcgccagcc tgcttccgaa tgggcccgct gcgaaagcag gactcgtcgc gttcgaccgg
     1141 ttggtttctg tcaacggaag ttcgctggtc ggcctcccgg ccgattcggc cgttcagttg
     1201 attcgcggac caaacggatc aactgcgaag ctcaccgtcg taacagcagg acacaagcga
     1261 gtagtctcgc tggtgcgaga cgctgttgca gtgccgagcg tgggcggagt cgcgctgttg
     1321 gacgacaaga tcggcgtggt gcgcctgcgt cagttcggcg aaggcgccac cggcgaagtg
     1381 gtgaacgcca ttgatgcgct ggtttcgtca ggcgcacgtg gaatcgtgct cgatctgcga
     1441 ggaaatcccg gtgggttgct ccaggaaggc ctgagcatcg cgcagttgtt cctgccttcc
     1501 ggctcgggcc tggtcgaagt tcgtccgcga tcgggacttc cggcccagcg ggcccgggcc
     1561 gaagctccgc cgcggtatcc caacctcccg ctggcggttc tgatagatgg tctgtcggct
     1621 tcggcttcag agatcttggc cggagctctt caggacgcgc accgcgccgt cgtgcttggg
     1681 cgccagagtt acggcaaggg ctccgttcag gaaaccgttc ctcttccgga gggttgggtc
     1741 gtcaagctga ctgtggcgcg gtggtacacg ccgtccggtc gcggaatcga tcgtgggcct
     1801 cagccggccg atgcgttgat tgatccagac gtcaggcatc cgggaggaat cgcccctgat
     1861 acgcctctcg cgccagacac tacggttgcg ccgccgcggc aagccatcgc gacgctgaag
     1921 aaatcgacct gggcgcgcgt gagcgaccgg ctggctgatt ggaccgaggc gcatcgcgcc
     1981 gactcgaccg cggcaccgac cagggaaacg gcggacactc tgttgtccgg cctggcgctg
     2041 ccagccagca tagagcctgc gttagccgac tggctcggtc gggctcttac ccaggagctg
     2101 ctgaccgcgc gatacggaga caccgccggt gaagcgtggt cgctgactca tgatgcggaa
     2161 gtggaggcgg cgcgcgcctg gctcgagcga cgactggcgg cgtaaaaaaa gaagatcccg
     2221 agactagtct cccaggctga ccacgccggc agcgcgccgc aggtaggtct cgagcgtcag
     2281 catggcgagc aaggttcgag tcttgatggg tgtttcgtgt cgccgctgaa gcgcggcccg
     2341 tagcgcaccg tccattactt cgtcggcgac aagatccgta gctgccatac gttcggccac
     2401 gcgggctgta gccgccaggc ggtcgtaatc ggcaaagttg agccgggccc gcgagattcc
     2461 gacccggaat cccgcccgct tcgccaatcg ccgcgcggca ctgacaaggg ctggctcggc
     2521 gagcccttct ccagtcttct gccacggcac agttcggaag aagcgcggat aggcttcgca
     2581 caagaatcgc gcgtagaaac ggtggccgct gcgctgcctg tcgtccaacc ctcccagcat
     2641 gagctgcaga atcggaaggc tcgcgccagg gtttaccacc tcgcagaacc ccatcatggc
     2701 cagcgggccc gtcagcgtcc atcgacgttg acccatcccg aggataaaac agtccacgct
     2761 ggggccaccc aggtatcggt cgcaatctcc gcgggacatc tcggccacct cggtggtgct
     2821 gaagaaggga ttgtcgtggg cccgctggct gagaaactcg tcccgcacgg cgatccagtc
     2881 cttcccgcct gccggttgcc gggcagtagc agggaaaagc cggtgcccgc ttcctccgaa
     2941 caggacatcc ccggtgctgt gcttcaaggt ccaggcattt cccatccgca tccgctcgag
     3001 cgccacggcg acatgaagat gcagtccgtt caccaacccg tcggtctgcc agatggct
//
LOCUS       DASRJQ010000661         3088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3867088, whole genome shotgun sequence.
ACCESSION   DASRJQ010000661 DASRJQ010000000
VERSION     DASRJQ010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3867088"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VFB89_15010"
     CDS             <1..781
                     /locus_tag="VFB89_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28656.1"
                     /translation="IRERTRPFGWSCETNADQALANDRKADAQPRGDLNRLQIAVTSE
                     DGDAWHVWFEPKLDFRKPAPYPDRRHLLAEIQSHVSIEKKTVASEDLLLRVTDGVPVL
                     TMADRLRRTTVDEWIDRSIHILSGKLVPGLVAPVDGLGEGCSLSVEQFRIEALQGPDA
                     LQRFMKGVLETPPWKAFKDDSRMFQVVGNGFESQRQIVRHVATSDLPKACGQYIAKIY
                     RLDVAEVRADPRSCVAWIELLLWGVNEAVTWRFDLRFPPPL"
     gene            complement(1011..2366)
                     /locus_tag="VFB89_15015"
     CDS             complement(1011..2366)
                     /locus_tag="VFB89_15015"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM28657.1"
                     /translation="MARHAEGWKLRPDKRRGGLYYVVFRRDGRRYMLATGERDLGEAT
                     LAAEALYASVVHGRRRPGVPVAQLLSAPGAQLDTLAAKWLADMEAQLDHTTCRQYEMY
                     VASHWQPYFKTIDRMTALSIEDYWRARLRKVQRRTVLKELSALRGLLSWCADRGHIAE
                     APTVKSPPARAHGTPDTKRPHKKQPVELEEHEVEAILAALPERSEGSPGRLGFVVRAR
                     FEVAWETALRPATLNELRVPSDYARGRTSLIIRDEVDKARFGRELPLSDRAREALDRV
                     CPEVGLIFGCHYELGKLLKQAAADAKLPKEKADAVSAYDLRHGRLTLLAERSPNLVGV
                     AYLAGHKQVTTTNRYLRTSKRAAEEVLAAAVAGRPGGPPTPPDDRQANRSTPPEAMPS
                     AEPIGMPHTETCSSGALRGTGWHSESEEVQADQHACARSGSCGVTPVRVRVSPFAPGL
                     S"
     gene            complement(2551..>3088)
                     /locus_tag="VFB89_15020"
     CDS             complement(2551..>3088)
                     /locus_tag="VFB89_15020"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HZM28658.1"
                     /translation="LESHHLWRRDLEAFGLQHIRVGIWRKEGATPQFTGDRPAVAAEA
                     IEIAGQLGALEIAHPFGRKRWSGGGEHAFWSAPIVLNRGRSKKRFHTTQKPDALMEAL
                     VRQFSEPGELVLDPFTGSGTTAVACRRLGRRFIGWEKDEGYAVRARVRIAETAEQRNL
                     FDDARAVPEQLLLLESGS"
BASE COUNT          534 a         1010 c          990 g          554 t
ORIGIN      
        1 gatcagggaa aggacacggc ctttcggatg gagctgtgag accaacgccg accaggctct
       61 ggcaaacgac cgtaaggcgg atgcccagcc gcgaggggat ctcaatcgcc ttcagatcgc
      121 tgttacttcg gaagacggag atgcgtggca cgtttggttc gagcccaagc tcgacttccg
      181 caaacccgca ccgtatcccg accgacggca cctgctggcg gagattcagt cacacgtgtc
      241 gatcgaaaaa aagaccgtgg cgtctgagga tctactgctt cgtgtgactg acggcgtgcc
      301 cgtactgaca atggccgatc gtttgcgaag gaccaccgtc gatgagtgga tcgaccggtc
      361 gatccacatc ttgagcggga agctggttcc aggcctggtg gccccagttg atgggctcgg
      421 cgagggatgc agcctcagcg tcgagcagtt ccgcatcgag gcccttcagg gccccgatgc
      481 cttgcagcgg ttcatgaagg gggtcctgga aaccccgcct tggaaggcct tcaaggatga
      541 ctcgcgcatg tttcaagtag tcggaaacgg cttcgagtcg caacgacaga tcgttaggca
      601 tgtggcaacg agcgacctgc cgaaggcttg cggacaatac atagcgaaga tctatcgtct
      661 ggatgtggcg gaggtgcgcg ccgaccctcg gtcatgcgtt gcctggatcg agctgctgct
      721 ctggggcgtc aacgaggctg tcacctggcg atttgatctg cggttccctc ctccgctgtg
      781 aaactcaagc tccaagatcc acctaagagc ggctcggtgt tcggcgcgtc catcggacca
      841 cgatagccgc aagcgcccac gagatcagcc tccggccgat cctcgccgtc ttagcccaca
      901 ggaacggggg ccaggagaaa gactccgcgg ctgcttccta ttagttggcc accgaggcca
      961 gccttggtga gattgtggcc gcggcggcca gaattccgag tgccgagatc tcagctaagt
     1021 cctggtgcga acggagagac tcgaactctc acaggtgtta ccccacagga acctgaacgg
     1081 gcgcacgcgt gttgatctgc ttgaacttcc tcggattcac tgtgccagcc ggtgccacgg
     1141 agtgccccgg aggagcacgt ttcggtatgc ggcatcccga tcggctcggc cgagggcatg
     1201 gcctccggcg gcgtgctccg gttcgcttgc cggtcatccg gcggcgtggg tggtccgccg
     1261 ggcctgccgg cgacggcagc agccaacacc tcctccgcgg ctcgcttcga ggtgcgcagg
     1321 taccggttgg tggtcgtgac ctgcttgtgg ccggcgaggt aggcgacccc caccaagttg
     1381 ggcgagcgtt cggccagcag cgtgaggcgg ccgtgccgca gatcgtaggc tgagaccgcg
     1441 tccgccttct ccttgggcaa cttggcgtcc gccgcggcct gcttgagcag cttgcccagc
     1501 tcgtagtgac acccgaagat cagcccgacc tccggacaca cccgatcaag agcttcacgg
     1561 gcgcggtccg aaaggggaag ctcgcgaccg aatcgggcct tgtcgacctc gtcgcggatg
     1621 atgagcgagg tgcgtccgcg ggcgtagtcg ctgggaacgc gtagctcatt cagcgtcgcc
     1681 ggccgcaagg ccgtctccca ggctacttca aagcgcgcgc gcacgacgaa accgaggcgg
     1741 cccggcgagc cctccgagcg ctcgggtagg gctgcaagaa tcgcctcgac ctcgtgctct
     1801 tccagctcca ccggctgctt cttgtggggg cgcttcgtgt cgggtgtgcc gtgcgcccgt
     1861 gcgggcggcg acttcacggt cggcgcctcc gcgatgtggc cacgatcagc acaccacgaa
     1921 agcagccccc gcaaagcgct cagttccttg agcaccgtcc ggcgctgtac cttgcggagt
     1981 cgcgcacgcc agtagtcctc gatgctgagc gcggtcatcc gatcgatggt cttgaagtac
     2041 ggttgccagt gactcgcgac gtacatctcg tactggcgac aggtcgtatg atcgagctgc
     2101 gcctccatgt ccgccagcca tttcgcggcg agggtatcga gctgtgcgcc gggagcgctg
     2161 agtagttgcg ctaccgggac gccgggacgc cgccgaccgt gtacaacgct ggcgtagagg
     2221 gcttccgccg cgagcgtagc ctctccaaga tcgcgctctc ccgtggcgag catgtagcga
     2281 cgaccgtccc gcctgaagac gacgtagtag agaccgccgc gccgcttgtc cggacgaagc
     2341 ttccagcctt ccgcgtgtct agccatcgct ccaagtccct ccggtcgaag aagatgcgac
     2401 taccgatcct gacaacggga atgctcgggc gcacatgccg ataaaaagtc cgccgactgg
     2461 ttcgacagta atcgatcgcc tcctggggag tgagcaaagg cgactctgat cgggccgtcg
     2521 agtgagtcgg atcacgattc gcgcccccaa tcatgacccg gactccagca gcaagagctg
     2581 ctcggggaca gcgcgggcgt cgtcgaacag gttccgctgc tctgccgtct cggcgatccg
     2641 tacgcgcgcc cggacagcgt agccctcgtc cttctcccag ccgatgaatc tgcgtcccag
     2701 cctgcggcac gcgactgccg tcgtgccgct cccggtgaag gggtcgagca ccagctcgcc
     2761 cggctcgctg aactgtctga ccagcgcctc catgagcgcg tccggcttct gtgtcgtgtg
     2821 aaaccgtttc ttgctcctgc cgcggttcag gacgatgggc gccgaccaaa aagcatgttc
     2881 gccaccgcca ctccacctct tgcgcccgaa cgggtgcgcg atctccagcg caccaagctg
     2941 gccggcgatc tcgatcgcct ctgccgccac tgccggccgg tcccccgtga actgcggggt
     3001 ggcgccctcc ttgcgccaga tcccgacccg gatgtgttgc agcccaaacg cctccaggtc
     3061 gcggcgccag aggtggtgcg actccaga
//
LOCUS       DASRJQ010000662         2833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1328017, whole genome shotgun sequence.
ACCESSION   DASRJQ010000662 DASRJQ010000000
VERSION     DASRJQ010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328017"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VFB89_15025"
     CDS             complement(<1..435)
                     /locus_tag="VFB89_15025"
                     /inference="COORDINATES: protein motif:HMM:NF015566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HZM28659.1"
                     /translation="MIAPPLVLVSLVVWGTLVGLDLVSVAQTMIARPFVAGGIAGVLL
                     GDPMAGLRAGVLLELFALDVLPVGAARYPDYGPATIAATVVLAGSPWELSLGLAGTVG
                     LLVSVLGGWSLQQLRRLNAEAVQRHTAALAAGDSDAIRRLQYA"
     gene            complement(432..908)
                     /locus_tag="VFB89_15030"
     CDS             complement(432..908)
                     /locus_tag="VFB89_15030"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HZM28660.1"
                     /translation="MPILVNRVDDRLIHGQVIVGWGRPLGIDLIILVDDQVSQSTWEQ
                     DLYRMAVPPEIELRFASIAEAARSLPEWQAASRRSLLLTGDIATMAALQAADGTVVQA
                     INLGGIHHRPGRTQRLPYLYLTDDELGCLRQLESRGATVTAQDLPTGMPVPLRSLG"
     gene            complement(908..1279)
                     /locus_tag="VFB89_15035"
     CDS             complement(908..1279)
                     /locus_tag="VFB89_15035"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28661.1"
                     /translation="MSDLQGVVVCHGALAGALVDAVEQVSGVRGALVPVSNSGCDRGR
                     LEERIVTAVGDRATVVFVDMASGSCLIAALAQLRTRPDVKVVTGVNLAMLVDFVFHLS
                     QTPEAAAARAASTGERAIKVP"
     gene            complement(1272..2234)
                     /gene="hprK"
                     /locus_tag="VFB89_15040"
     CDS             complement(1272..2234)
                     /gene="hprK"
                     /locus_tag="VFB89_15040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HZM28662.1"
                     /translation="MLRLRDFLTRRGDPLQLEALTGELGLDRQLPDAEVASPGLALAG
                     YTGRFAPNRLHVLGETEITYLRSLPPAERLHRLEHFFTFDLPCVFVTKGQEVPQELLD
                     LAKQRGVPVLRSRLKTAEFYRRVKPIVEDAFAPRTTLHGSLADVYGVGLLFVGRSGIG
                     KSECVLDLVERGHRLVADDVVQVTRRGHDVLIGRGHELAAHHMEIRGIGLIDIPALFG
                     VRAVRQQKRIEVIVQLEDWETAKDSDRSGLTRQDTMILEVPVPKVVVPLNPGKNITVI
                     SEVVAMMHLLRYSGVDVAAAFNERLIRRMKERSGVKEYLQDDYE"
     gene            complement(2234..2548)
                     /locus_tag="VFB89_15045"
     CDS             complement(2234..2548)
                     /locus_tag="VFB89_15045"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HZM28663.1"
                     /translation="MQTTITVRHCEISDALRERALAVSDRLATFAPRPLEATVVFDAD
                     GLQRVVELRLHVARGEILVAKGEAGDHRTALDRAEDKLRRQLEKTNGRPRRKRTPPTP
                     TS"
     gene            complement(2567..>2833)
                     /locus_tag="VFB89_15050"
     CDS             complement(2567..>2833)
                     /locus_tag="VFB89_15050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZM28664.1"
                     /translation="VEAGACGTPSLASDSPGLRDSVRHGETGYLVRHGDEQALADRML
                     ELAAAPSLVERFGAAARRFAEAHTWERSAQATERHLQAIIAGSA"
BASE COUNT          508 a          955 c          916 g          454 t
ORIGIN      
        1 ggcgtactga agccgacgaa ttgcgtcgct atcacccgcg gcgagcgctg ccgtgtgccg
       61 ctgcactgcc tccgcgttga gtcggcggag ctgctggaga ctccatccgc ccaggacgga
      121 gaccagaagt cccacggttc cggcaagccc caggctcaac tcccagggag agccggcgag
      181 aactacggtc gccgcgatcg ttgccggccc gtaatcgggg tagcgagcgg cgccgaccgg
      241 aaggacgtcg agggcaaaaa gctcgagcag cacgcccgca cgaagccccg ccataggatc
      301 acccagcagc acgccggcaa tgccgcccgc gacgaacggt cgcgcgatca tggtctgggc
      361 gacgctcacg aggtcgagcc cgaccagcgt gccccacacc acgagcgaca ccaggaccaa
      421 gggtggcgcg atcatcccaa gctccgaagc gggacaggca ttccagtcgg aagatcctgg
      481 gcggttaccg tggcgcctct tgattcgagt tgccgcaggc agcccaactc gtcatcggtg
      541 agatagaggt aaggcagccg ctgggtgcgg ccgggacgat gatgtatccc gccgagattg
      601 atcgcttgca ccaccgttcc gtcggccgcc tgaagcgcgg ccatggtggc gatgtctccg
      661 gtgaggagaa ggcttcggcg ggaagcggcc tgccactcgg gaagtgagcg ggccgcttcg
      721 gcgatgctcg cgaagcgcag ctcgatctca ggagggacgg ccatccgata caaatcctgc
      781 tcccaggtgc tctgggacac ctggtcgtcc accagaatga tcagatcaat tcccagcgga
      841 cggccccagc ccacgatcac ctgcccgtga atcaggcgat cgtccacccg gttgaccagg
      901 attggcatca gggaaccttg atggcccgct cccctgtgct cgcggcacgg gccgcggccg
      961 cctcgggagt ctgggacaga tgaaagacga aatcgaccag cattgccaga ttgacgccgg
     1021 taaccacctt gacgtcgggt cgggtgcgga gctgggccag cgccgcaatg aggcaggatc
     1081 cgctggccat gtccacgaat accacggtcg cccggtctcc aacagcggtg acgatgcgct
     1141 cctctaatcg accccgatca cacccggaat tcgaaacggg caccagcgcc ccgcgcactc
     1201 cgctcacctg ctcgaccgca tctaccagcg cgcctgccag tgcaccatgg cagacaacta
     1261 ccccctgcaa gtcactcata gtcgtcctgc aggtattcct tgaccccact ccgctccttc
     1321 atccgacgga tgagccgctc attgaaggcc gccgcgacgt ccacgccgga ataccgcagc
     1381 aaatgcatca tcgcgacgac ctcggagatc acggtgatgt tcttgccggg gttgaggggg
     1441 accacgacct tggggacggg aacctcgagg atcatggtat cctgacgggt gagcccgctg
     1501 cggtcgctgt ccttggccgt ctcccagtcc tccagctgca cgatcacctc gatcctcttc
     1561 tgctgacgga ccgcgcgcac accgaagagc gccggaatgt cgatcaggcc gatgccgcgg
     1621 atttccatgt ggtgggctgc cagctcgtgg cccctgccga tcaggacgtc gtgcccccgc
     1681 cgggtgacct gcacgacgtc gtcggccacc aggcgatggc cccgctcgac cagatccaga
     1741 acgcattcgc tcttgccgat gcccgaccgg ccgacgaaaa gcagcccgac cccatagacg
     1801 tcggccaatg agccgtgcag cgtggttcgc ggggcaaacg cgtcttcgac gatcggtttg
     1861 acccgccgat agaactcggc ggtcttgagg cgactccgaa gcacggggac gcctcgctgt
     1921 ttggcgagat ccaggagctc ctgaggtacc tcctgcccct tggtgacgaa cacacacggc
     1981 agatcgaacg tgaagaagtg ttcgaggcgg tgaagacgtt ccgccggagg gagcgagcgg
     2041 aggtaggtga tttcggtttc accaaggacg tggagccgat tcggcgcgaa gcgaccggtg
     2101 tagcccgcca aggcgagccc tgggctcgcg acttccgcgt ccggcagctg gcggtcgagt
     2161 cccaactcgc cggtgagcgc ctcgagctgg agcgggtcgc cgcgccgggt cagaaagtcg
     2221 cgcagtcgca gcatcaactc gtcggggtcg gcggagtacg tttgcggcgg ggccgtccgt
     2281 tggtcttttc cagctgacgg cggagtttgt cttcggcccg atccagcgcc gtgcgatggt
     2341 cgccggcctc acccttggcc accaggattt cgcctcgggc cacatgcagc cgaagctcga
     2401 ccactcgctg gagcccatct gcgtcgaaga cgacggtggc ttcgagcggc cggggcgcga
     2461 acgttgcgag ccggtcactg accgcgaggg cgcgctcccg aagagcgtcg ctgatctcgc
     2521 agtggcgcac cgtaatcgtc gtctgcatgt cccctccctt cccggcttag gccgagcccg
     2581 cgattatggc ttgcagatgc cgctcggtgg cttgagctga tcgctcccag gtgtgggcct
     2641 cggcgaaccg gcgggcagcc gccccgaaac gctccaccag ggagggcgcg gccgcaagct
     2701 cgagcatccg gtctgccaag gcctgctcat caccatgccg cacgagatag ccggtttcgc
     2761 cgtgacgaac cgagtctcgc aggccggggc tgtccgatgc cagagatggg gttccacagg
     2821 ctcctgcctc gac
//
LOCUS       DASRJQ010000663         4049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_316749, whole genome shotgun sequence.
ACCESSION   DASRJQ010000663 DASRJQ010000000
VERSION     DASRJQ010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316749"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..1742)
                     /locus_tag="VFB89_15055"
     CDS             complement(114..1742)
                     /locus_tag="VFB89_15055"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HZM28665.1"
                     /translation="MSGHFDIIIIGTGAGGGTLAYRLAPSGKKILLLERGDYVPREPD
                     NWSPRAVNVEGKYQTREKWQDQDGKPLHPHTNYYVGGNTKFYGAALFRLRQQDFGEIR
                     HHGGISPAWPITYDELEPYYAEAELLYHVHGERGEDPTEPRTTRAYPHPAVSHEPRIQ
                     QLADHFAAHGLRPFHTPLGVMLNESNPRRSKCIRCATCDGFPCLVQAKSDAQVCCVDP
                     ALEYANVTLVTGAYVSRLETDASGRQISKVHVKRGDATETYTGDIVVSSCGAINSAAL
                     LLRSANDKHPNGLANGSDVVGRHYMGHINSVLMAVSKCPNPTVFQKTLSVNDFYAGSK
                     EWEYPMGHISFVGKLDGITLSAGAPKIAPGFTLDLMGKHSLDFWLTSEDLPDPDNRVT
                     LSGDGQIMLSYAPNNGEGHKRLTAKLEHLMNEQSSCPVHGKSCHVGLFSRNLFVGQRI
                     PLAGVAHQNGTTRFGRDPKTSALDPTCKAHELDNLYVVDGSFFPSSAAVNPALTIMAN
                     ALRVGDRILERLGGSGRERAGADGSRREQTGALA"
     gene            complement(1824..2555)
                     /locus_tag="VFB89_15060"
     CDS             complement(1824..2555)
                     /locus_tag="VFB89_15060"
                     /inference="COORDINATES: protein motif:HMM:NF038123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spondin domain-containing protein"
                     /protein_id="HZM28666.1"
                     /translation="MSLIRALSAATLLVVVASGAAAQQEAAKPTTFTIRVENATSGEA
                     LKLSNGKTAPLVIAPVLYVVHTSNTNPLFAGGQLDAGKGLRTLAETGNPEPLSKSVAG
                     QTGVMSVAADAKPVGATEGGPLPPGKAYEFTVAAEPGQFLSIASMFGQSNDWFYSNDR
                     PIALFVNGKPVSGDMSAQISLWDAGTEVDEEPGLGPNQGPRQKDPQAGVRENQAIAHV
                     NSKWVYPKTGFKLTVTPSGDAMSQR"
     gene            complement(2573..3061)
                     /locus_tag="VFB89_15065"
     CDS             complement(2573..3061)
                     /locus_tag="VFB89_15065"
                     /inference="COORDINATES: protein motif:HMM:NF021996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HZM28667.1"
                     /translation="MNRRVWIAGLVVLGGVAWYLFRPELLFVNKRVNEGISTGSAAAE
                     TGAVPARLVGGSFHSVAHETHGTATILDVSGKRILRLTDFTTSNGPDVRVYLVAASDA
                     SDNETVTKAGFVELGKLKGNQGDQNYDVPSNLDLAKYRAVTIWCRRFSINFATAPLTP
                     AS"
     gene            complement(3222..>4049)
                     /locus_tag="VFB89_15070"
     CDS             complement(3222..>4049)
                     /locus_tag="VFB89_15070"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HZM28668.1"
                     /translation="AVTAVKSSAAAHRSHWPEYAIEAALLGLFMVSACGFTVLLEHPA
                     SPVYRAIPSDFVRRSLTGLAMGGTAITLIYSGWGQRSGAHFNPAATLTFFRLGKVAPK
                     DALGYVVAQFVGGVLGVMLSSAVLGELLAHQRVRYAVTLPGIYGASLGWAGPGVAWVA
                     EFVISFVLMSVLLQVMNSRLSRFTGLFAGALVATYITLEAPISGMSMNPARTFASAFG
                     AHNWTAVWIYFTAPPLGMLAAAEAYLRRRGQRAVGCAKLYHDNHKPCLFCEFHGTKA"
BASE COUNT          720 a         1296 c         1293 g          740 t
ORIGIN      
        1 aggctgggtg tattgagccg ccgccgaact gccgctggtg accagcgcca gcgctgagag
       61 ggcgagtccc ccgcgctcga tccagcccct ggcaacccgg aagacctcgc tcctcatgcc
      121 agagcacccg tctgctcccg cctgctccca tctgctcccg cccgctcccg tccgctcccg
      181 cccagccgtt cgaggatccg atcgccgacc cgcagcgcgt tcgccatgat ggtgagcgcc
      241 ggattcacgg cggcgctcga gggaaagaag ctcccgtcca ccacgtagag gttgtcgagc
      301 tcgtgcgcct tgcaggtcgg gtccagtgcg gaggtcttgg gatcgcgccc gaaccgagtg
      361 gttccgtttt gatgggcgac gcccgcgagc ggaatccgtt gccctacaaa cagattccgc
      421 gagaagagcc cgacgtggca gctcttgcca tggaccgggc agctcgactg ctcgttcatc
      481 aggtgctcga gcttcgcggt gagccgctta tgcccttcac cgttgttggg cgcgtaggag
      541 agcatgatct ggccgtcccc gctcaaagtc acacgattgt cgggatcggg cagatcctcc
      601 gatgtcagcc agaaatcgag cgagtgcttg cccatgagat cgagggtgaa gccgggcgcg
      661 atcttggggg cgccggccga cagggtgatg ccatccagct tgcccacgaa cgagatgtgc
      721 cccatcgggt attcccactc cttggatccc gcatagaaat cgttcacgct gagggtcttc
      781 tggaagacgg tcggattggg gcacttggag accgccatca ggaccgagtt gatatggccc
      841 atgtagtggc ggcccaccac atcggagccg ttggcgagtc cgttggggtg cttgtcgttg
      901 gccgagcgaa gcaggagcgc cgccgagttg atggcaccac aggaggaaac cacgatgtca
      961 cccgtgtagg tctcggtcgc gtcgccccgc ttgacgtgga ccttgctgat ttgtcgcccg
     1021 gaggcgtcgg tctccaaccg ggatacgtag gcgcccgtca ccagcgtcac gttggcgtac
     1081 tcgagcgccg gatccacgca gcagacttgg gcgtcggatt tggcctgcac caggcagggg
     1141 aagccgtcgc aggtggcgca gcgaatgcac ttgctccggc gtgggttgct ctcattgagc
     1201 atgacgccga gcggcgtgtg aaacggccgc agcccatgtg ccgcgaagtg gtcggcgagt
     1261 tgctgaatcc tgggctcgtg gctcacggcc ggatggggat aggcccgcgt ggtccgcggc
     1321 tcggtcgggt cctcgcctcg ctcgccgtgt acatggtaca gcagttcggc ctccgcgtag
     1381 taaggctcaa gctcgtcgta ggtaatcggc cacgccgggg aaataccgcc atggtggcga
     1441 atctcgccga agtcctgctg gcgcagccgg aacagcgctg cgccgtagaa cttggtgttg
     1501 ccgcccacgt agtagttggt gtgagggtgc agcggctttc cgtcctggtc ctgccacttc
     1561 tccctggtct gatacttccc ctcaacgttc accgcgcgag gactccaatt gtccggctcg
     1621 cgaggcacgt agtcgccgcg ctcgagcagg agaatcttct tgccggaagg agcgagccgg
     1681 tatgccagcg ttccgccgcc ggccccagtg ccgatgatta tgatgtcgaa atgtccggac
     1741 atgttggcct caacagaaag cgtgaacggt gaacaggggc tcagctcacc ctgcagtcgc
     1801 gttcaccgtt caccgtttac cgttcaccgc tgcgacattg cgtcgccgga cggcgtcacc
     1861 gtcaacttga atccggtttt gggatagacc cacttgctgt tcacatgggc gatcgcctgg
     1921 ttctcgcgga caccggcttg cgggtccttt tgccgcggac cctggttggg accgagccca
     1981 ggttcctcgt ccacttcggt gcccgcatcc cacagggaga tttgcgcgga catgtcgccg
     2041 ctcaccggct tgccgttcac gaacagagcg atgggccggt cgttggagta gaaccagtcg
     2101 ttcgactgcc cgaacatcga cgcaatcgac aggaattggc cgggctccgc agccaccgtg
     2161 aactcgtacg ccttacccgg aggaagcgga ccgccttcgg tcgcgccgac cggcttcgca
     2221 tcggctgcga ccgacatcac tcccgtctgc cccgcgacgg acttgctgag tggctcagga
     2281 ttccccgtct ctgctaacgt ccggagcccc ttgccggcat cgagttggcc tccggcgaac
     2341 agcgggttgg tgttgctggt gtggacgaca tagagcacgg gcgcgatcac taagggcgcc
     2401 gtcttgccgt tcgacagctt gagcgcctca ccgctggtgg cgttctcgac ccggatggtg
     2461 aaggtcgtgg gcttcgccgc ctcctgctgc gccgcggcac cactggcgac gacgacgagg
     2521 agagtggcag cggacagtgc gcgaatcagc gacatggtcg gtctcctcgg cttcatgaag
     2581 ccggcgtgag cggtgccgta gcgaagttta tgctgaaacg acggcaccaa atggttacgg
     2641 cgcgatactt ggcgaggtcg aggttactgg ggacgtcgta gttctggtcg ccctggttgc
     2701 ccttgagctt gcccaattca acgaacccgg ccttcgtcac ggtttcgttg tccgaggcgt
     2761 cgctggcggc caccaggtac acccgcacgt cgggaccgtt ggacgtggta aagtcggtca
     2821 gtcggaggat ccgcttgcca ctcacgtcaa gaatggtggc cgtcccatgc gtctcgtgag
     2881 cgaccgaatg gaagctccct cccaccagcc gtgccgggac ggcccctgtc tccgccgccg
     2941 cagagccggt cgagatgccc tcgttgaccc gcttgttcac gaacaggagc tcgggtcgaa
     3001 agaggtacca ggccacgcct ccgagcacga cgaggccggc aatccatacg cgacggttca
     3061 tcagatcact ccttagagag ttcagttgcc gggtgcgaga tgacgggtat aaagccggct
     3121 cgcaggaaat agttcccagc ttaaaaagcg tgaacagtga acagtgaaaa ggggcgcccg
     3181 atgatcaccg gagcccccgt tcaccgttca ctgtttactg tttacgcttt tgtcccgtga
     3241 aactcgcaga acaaacaggg cttgtggttg tcgtggtaca gcttggcgca accgactgcg
     3301 cgttggcccc ggcgtctcag ataggcctcc gccgccgcca gcatgcccaa gggcggcgca
     3361 gtgaagtaga tccagaccgc ggtccagttg tgggcgccga acgccgaggc gaacgtgcgg
     3421 gcggggttca tgctcatgcc cgaaatcggc gcctccagcg tgatataggt ggccaccagt
     3481 gccccggcga acaagcccgt aaagcgcgac agccgcgagt tcatcacttg tagaaggacg
     3541 ctcatcagca cgaacgaaat cacgaactcc gccacccagg ctacgccagg tccagcccag
     3601 cccaggctgg ctccataaat gccgggaagc gtgaccgcgt agcgcaccct ttggtgcgcg
     3661 agcagctccc ccagcaccgc actggagagc atcacgccaa gcaccccgcc gacgaactgc
     3721 gccacgacat atcccagggc gtccttcggg gccaccttgc ccagccggaa aaaggtcaac
     3781 gtcgcggcgg gattgaagtg ggcgcccgag cgttgtcccc aacccgagta gatcagcgtt
     3841 attgccgtac cacccatcgc cagaccggtg agcgagcggc ggacgaagtc gctgggaatc
     3901 gctcggtaga ccggcgacgc aggatgctca agcagcacgg tgaacccgca cgcagagacc
     3961 atgaacagcc cgagcaatgc ggcttcgatc gcgtattccg gccagtggct ccggtgggcc
     4021 gcggcactac tcttcaccgc ggtgaccgc
//
LOCUS       DASRJQ010000664         3248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1529629, whole genome shotgun sequence.
ACCESSION   DASRJQ010000664 DASRJQ010000000
VERSION     DASRJQ010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1529629"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /locus_tag="VFB89_15075"
     CDS             <1..1307
                     /locus_tag="VFB89_15075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28669.1"
                     /translation="AQQNSCVGVTAACSLGPNVTTSQIQIGGPGSISSNNTVPATCDG
                     GTPGNLISARYDFSFDRSAGQLTVTVANTTQAPSAGALTGFGFNVTSDVTLITLASQT
                     AGGLSWSAAFDRDRTDNSIEVPSGSNSKDIKCDGFGRVSAFAGNKGVDTGFGGGDAIE
                     ILPGNSKTYVFNVSGNLANVTACSFTSVASLIPPGDKTAIAIGRFQACNGGGSAWAGP
                     CTPTNLLVNLVDFKVKGNRNEQIEWETSSEINSAGFYVLARDERTKEYVRVNQNLIPA
                     RGTEVSGAFYTLQDSAAVNGKKKKYILEEIELDGNNNIYFPRAQVVVINPLNPDINLL
                     SPAYEELVSTKGGVRLSWESANFSRKQVIEISSDATFPEAGTLSLNAGATKTRSLSRS
                     ELNQVADMATLSGEGGVYWRVRGKNVAGGEGTSQTFFMPVQQ"
     gene            1336..1668
                     /locus_tag="VFB89_15080"
     CDS             1336..1668
                     /locus_tag="VFB89_15080"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HZM28670.1"
                     /translation="MATGCPRTRRDLVLQEVGREGLLYDKEGELIHILNTTAIEIWRV
                     CDGGKDVSAIESVIREKFTNAAGQDVRGDIEKLLTQLDERGLLEGDGAHPTAAPRPGT
                     GAGPHRRG"
     gene            1676..1807
                     /locus_tag="VFB89_15085"
     CDS             1676..1807
                     /locus_tag="VFB89_15085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28671.1"
                     /translation="MNNKEFDAPVAYVTPTIETLTDRDILEELGPAQAYTGTLPFQF"
     gene            1946..3118
                     /locus_tag="VFB89_15090"
     CDS             1946..3118
                     /locus_tag="VFB89_15090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28672.1"
                     /translation="MKTTRPHTETAERYCLPGIRFSLATDSVEVRDFFRASYRHFPDA
                     EGPVSDCFPLTALLHADDGRAVATAGDERLELSGRPMPVNRAFLFLLNAIMERISDFI
                     LLHGAAVSVNGRGIVLAGPPTAGKSTLVLELARRGAAFLSDDVAPLSRATGLLHPFPR
                     AIGIRREGELLSRIDRSALPPESVHELAYKWLVDPRALGLRLAGPGDAPCPLDAIFLL
                     DLDEMESAPGRDRYEIALAEEDHDVLSEVRSVPGVLDLRRHEDRPFPVYTFRIDAARR
                     PMGPLADILRRRRDVVLYMDASRPGHPERFEQPVVQEARTMTVVMELARDLLNRSESG
                     ELLKSCGGLSGLVAEMGSLLKGARTYRLRPGNPGRTAELLLAMATGRDGERMKGES"
BASE COUNT          640 a         1043 c         1045 g          520 t
ORIGIN      
        1 ccgctcagca gaacagctgc gtcggtgtca cggcggcctg cagcctcggc cctaacgtca
       61 cgaccagcca gatccagatc ggcggtcccg ggtcgatcag cagcaacaac accgtgcctg
      121 ccacctgtga cggtggcacg cccggcaacc tgatcagcgc gcggtatgat ttcagcttcg
      181 accgcagcgc cggccagctg accgtgacgg tcgccaacac cacgcaggcc cccagcgcag
      241 gagccctgac agggttcggc ttcaacgtga catcggacgt cacgctgata accctggcat
      301 cccagacggc cggcggcctg agctggagcg ccgcgttcga ccgtgaccgc accgacaatt
      361 cgattgaagt ccctagcggc agcaacagca aggacatcaa gtgcgatggc ttcggccgcg
      421 tgagcgcgtt cgccggcaac aagggcgtcg acaccggctt cggcggcggc gacgcgatcg
      481 aaatcctgcc cggcaactca aagacctacg tcttcaacgt gagcggcaac ctggccaacg
      541 tcaccgcgtg ctcgttcact tctgtcgcca gcctgattcc tccgggcgac aagacggcga
      601 tcgcgatcgg ccggttccag gcctgcaacg gcggcggttc ggcgtgggcc ggcccctgca
      661 cgccgacgaa cctgctggtc aacctggtcg acttcaaggt caagggcaac cgcaacgagc
      721 agatcgaatg ggaaacttcc tctgaaatca acagcgccgg cttctacgtg ctggcgcgtg
      781 acgagcggac caaggagtac gtccgcgtca accagaatct gattccagct cgcggcaccg
      841 aagtttccgg cgccttctac accttgcagg attcggccgc cgtcaacggc aagaagaaga
      901 agtacatcct cgaagagatc gagctggacg ggaacaacaa catctacttc ccgcgcgccc
      961 aggtagtcgt catcaatccg ctgaaccccg acatcaacct gctgtcgcct gcgtacgaag
     1021 agctggtcag cacgaagggc ggggtgaggc tgagctggga atcggccaat ttctcgcgca
     1081 agcaggtgat cgagatctcg agcgatgcga ccttccccga ggctggcacg ttgtcgctga
     1141 acgccggcgc gaccaagacg cgctcgctgt ctcgcagtga gctgaaccag gtcgcggaca
     1201 tggcgacgct gagcggcgag ggtggcgtct actggcgcgt tcgcggcaag aatgtcgccg
     1261 gcggcgaggg aaccagccag accttcttca tgcccgtgca gcagtaagag caagggaggc
     1321 gaaagcttga tcgcaatggc cacaggatgc cccaggacgc gcagagacct ggtcctgcag
     1381 gaagtcggca gggagggtct gctgtacgac aaggaagggg agctgatcca catcctgaac
     1441 accacggcga tagagatttg gagggtctgc gatggcggca aggatgtctc ggccatcgag
     1501 tcagtcatca gggagaagtt cacgaacgcc gcaggccagg acgttcgcgg agacatcgag
     1561 aagctcttaa cgcaactaga cgagcggggg ctgctggagg gcgatggagc ccatccaacg
     1621 gccgcaccgc gaccggggac cggagcaggt cctcataggc ggggatgagg cgagcatgaa
     1681 caacaaggag ttcgacgctc cagtggctta cgtcacgccg accatcgaga ccctcacaga
     1741 tcgggatatc ctcgaggagc tcgggccggc gcaggcgtac accggaacat tgccgttcca
     1801 gttctaacag gcggcttgca agagtaagag taagaagaga gcaaccaatc aagggaatga
     1861 agcagaggag gatgacacct ccgtcgtcgc ttcattccct tttggttttt cagggcttcc
     1921 gagaaatagg atcgcaacgg tcaccatgaa aacgacaagg ccccacaccg aaaccgccga
     1981 gcgatactgc ctgccgggca tccgcttctc gctcgcgacc gacagcgtcg aagtgcggga
     2041 tttcttcagg gcctcctacc gccacttccc cgatgcggaa gggccggttt ccgactgctt
     2101 cccgctgacc gccctgctcc acgccgacga cggaagagcc gtcgcgacgg cgggcgacga
     2161 gcgcctcgag ctctcggggc ggccgatgcc ggtgaaccgc gcgttcctgt tcctgctgaa
     2221 cgccatcatg gaacggatct cggatttcat cctgctgcat ggcgcggcgg tttccgtgaa
     2281 cgggcgcggg atcgttctcg cggggccacc gacggccggc aaatcgacgc tggtgctcga
     2341 gcttgcgcgg cgaggcgcgg ccttcctgag cgatgacgtg gcgccgctga gcagggccac
     2401 gggcctgctg catccctttc cacgggccat cggcatccgg cgggagggtg agcttctttc
     2461 ccgcatcgat cggtccgcgc tgccgcccga atcggttcac gagctggcct acaaatggct
     2521 cgtcgaccca cgtgcgctgg ggttgcggct ggccggaccc ggcgacgcgc cctgcccgct
     2581 cgacgcgata ttcctcctgg acctggacga gatggagtcg gccccggggc gggatcgcta
     2641 cgaaatcgcg ctcgccgaag aggaccacga cgttctctcc gaggtgcgct ccgtgcctgg
     2701 ggtgctcgac ctccggaggc acgaggacag gcccttcccg gtctatacct tcaggatcga
     2761 tgccgcccgg cggcccatgg gccccctcgc cgacatcctc aggcgtcgcc gcgacgtcgt
     2821 cctctacatg gacgcgtcca ggcctggcca tcccgaacgt ttcgaacagc cggtggtcca
     2881 ggaagcccgc acgatgacgg tggtcatgga gctggcgcgc gacctgttga accgttcgga
     2941 atctggagag ctgctgaaat cctgcggcgg gctttcgggt ctcgtcgccg agatgggaag
     3001 tcttctcaag ggggcgagga cctaccgcct gcgccccggg aatcctggaa gaacagccga
     3061 actgctcctc gcgatggcga cggggcggga tggcgaacgc atgaagggag aatcatgaac
     3121 cggtccaccc ggcgcctcaa ccttgtcagg caagcgacga tgctgttcgc gatcacggcg
     3181 tccacattca tcacgagctg gagacccgcc tcggcctcta gcctgagcag cagcaagctc
     3241 ggggatgg
//
LOCUS       DASRJQ010000665         2065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_969905, whole genome shotgun sequence.
ACCESSION   DASRJQ010000665 DASRJQ010000000
VERSION     DASRJQ010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969905"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VFB89_15095"
     CDS             complement(<1..462)
                     /locus_tag="VFB89_15095"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZM28673.1"
                     /translation="MRLEAIGCCRASGVIAALALVLLVHSPLAAQECKPTRMALVLSG
                     GGAKGFVHIGVLEVLDSAGIRPDLIVGTSMGAIVGALYASGYTGHEIDSLFRALPLTQ
                     LIRGYEPRIPESLRPYPPLAVLEEGRNNLILQTSTVREEQVNALVNALMLRG"
     gene            complement(459..1619)
                     /locus_tag="VFB89_15100"
     CDS             complement(459..1619)
                     /locus_tag="VFB89_15100"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28674.1"
                     /translation="MRTQSYDCYAITPPGVESITARELSALGIAPGESEPGGVSFRSD
                     AGGIARANLELRTASRLLVRVATFHASAFHELERRAGRLAWEAYVPPGRSVAFRVTSR
                     KSKLYHQGAISQRLGESVMRRVPGATVSSGGMDSEERATAESGPQLFVVRLFHDECMV
                     SADSSGEHLHHRGYRLVGAKAPLRETLAAAMLLAAAWDGTRPLIDPLCGSGTIPIEAA
                     LLARRIAPGLRRAFACEQWPAFPRSVVASARADAQERILAQAPAPILGSDRDAGAIDA
                     AFENAARAGVPESIQFRKQAISALEPPPGPGFVVTNPPYGVRVGQSRDLRDLYAQLGN
                     VLRRRCSGWRVALLSARDELARQTRLPLAAAFRTSNGGLRVKLLLATIPPAA"
     gene            complement(1636..>2065)
                     /locus_tag="VFB89_15105"
     CDS             complement(1636..>2065)
                     /locus_tag="VFB89_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28675.1"
                     /translation="TSRALAHHDDSQFQAAVAAGDVLVQPLISAVATQGEWSVICFGG
                     TPSHAVLKRPAAGDWRVQGELGGTAQLRTAPPALIKDARRVLSAAGAEQAAYARVDGY
                     LDDDRLVLMELELIEPQLYLDLEPEAPSRFAGAIAATLRS"
BASE COUNT          366 a          712 c          668 g          319 t
ORIGIN      
        1 gccgcgcagc atgagcgcgt tcaccagtgc gttgacctgc tcttccctga cagtgctggt
       61 ctgaagaatc agattgtttc ggccctcttc aagcactgca agcggcggat aggggcgaag
      121 cgactcggga atgcggggct cgtatccgcg gatgagttgg gtaagcggca gcgcacggaa
      181 gagtgaatcg atctcgtgtc cggtatatcc gctggcgtac agcgccccga cgatcgcgcc
      241 catgctggtg ccgacgatta gatccggcct gatgccggca ctatccagca cctcgagcac
      301 gccgatgtgg acgaacccct tggcccctcc ccctgacagc accagcgcca tcctcgtcgg
      361 cttgcactct tgagccgcga gcggagaatg caccagcaac accagggcga gcgcagcaat
      421 gaccccgctt gccctacaac agccgatcgc ctcgagcctc atgcggccgg cggaatcgta
      481 gcgagcagca gcttcacgcg gagtccgccg ttgctggttc gaaacgccgc cgcaagcggc
      541 agcctggtct gtcgagccag ctcgtcacgg gcggagagta gggctactcg ccacccggaa
      601 cagcggcgtc gcagcacgtt gcctaactga gcatagaggt cgcgcagatc ccggctttgc
      661 ccgacccgga cgccgtaggg cgggttggtg acgacgaatc ccggcccagg aggcggttcg
      721 agcgcggaga tcgcctgctt gcggaactgg atgctttcgg gaacaccggc ccgggcggcg
      781 ttttcaaacg ccgcgtcaat ggcacccgcg tctcggtccg agcccaggat gggggccggc
      841 gcttgggcaa ggatacgctc ctgcgcgtct gctcgcgctg acgccacgac cgatctgggg
      901 aaggccggcc actgctcgca ggcaaacgcg cgacgcaggc caggcgcgat ccgtcgcgcc
      961 agcagcgcgg cctcgatcgg tatggtaccg gacccgcaca gtggatcgat caacggcctg
     1021 gttccgtccc aggctgcggc cagcagcatt gctgccgcga gggtttcccg aagcggagcc
     1081 ttggcaccca ccagccggta cccacggtga tgaaggtgct cgccggaact gtcggcactc
     1141 accatgcact cgtcatgaaa cagccgcacg acgaagagtt gaggtccgga ctcagcagta
     1201 gctcgctcct ctgaatccat tccaccggat gagacggtag cgcccggaac tcgccgcatg
     1261 accgactcgc ccagcctctg cgagatagca ccctgatggt agagcttcga ctttcgggag
     1321 gtgacccgga acgccaccga tcgcccgggc gggacatacg cctcccacgc gagacgaccc
     1381 gcgcgccgct ccagctcatg aaacgcagat gcatgaaagg tggcgactcg cacgagcagc
     1441 cgagatgccg tgcgcagctc caggttcgcg cgcgcgatcc cacccgcgtc gctccgaaag
     1501 ctcacgcctc ccggctcgct ctcgccggga gcgatgccca gcgccgacaa ctcgcgggcc
     1561 gtgatcgact ccacccccgg cggggtaatg gcgtagcagt cgtagctctg cgtccgcatg
     1621 aaaggctggg ccgcctcagc tccgcagcgt cgccgcgata gcaccggcga accggctcgg
     1681 cgcctccggc tcgagatcga ggtagagctg gggctcgatc agctcgagct ccatcaatac
     1741 cagccgatcg tcgtcgaggt agccgtctac cctggcatat gctgcctgct cggccccggc
     1801 tgccgacagc actcggcggg cgtctttgat gagggcaggc ggcgccgttc ggagctgagc
     1861 agtcccacca agctcgcctt ggacccgcca gtcgcctgcg gcgggccgct tcagaacggc
     1921 gtggctcggc gtaccgccga agcagatcac ggaccattcg ccctgcgtcg cgaccgcgga
     1981 gatcagcggc tgcaccagca cgtcgccggc cgctaccgca gcctggaatt gcgaatcgtc
     2041 gtggtgcgcc aaagctcgcg aggtg
//
LOCUS       DASRJQ010000666         4330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_682649, whole genome shotgun sequence.
ACCESSION   DASRJQ010000666 DASRJQ010000000
VERSION     DASRJQ010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682649"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VFB89_15110"
     CDS             <1..244
                     /locus_tag="VFB89_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28676.1"
                     /translation="FQREIRFAARLQHHLNQAIDHLSRAIEPGWRDLAMLDHGSEFQR
                     YQSDPVLRELRTGVEALPKVPVPAAIEGLASRLGDA"
     gene            237..2048
                     /locus_tag="VFB89_15115"
     CDS             237..2048
                     /locus_tag="VFB89_15115"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZM28677.1"
                     /translation="MLRRRLAVCLSAVAVAGLSGVSLKAQKLEPVRYILRIPAPQTHY
                     IEVEATYPTSGRPTVDLMMAVWTPGSYLIREFERHVESMRARDTADRALELEKTRKNR
                     WRVYTGGARTIRLNYRVYANEHQARTDWVEDSFALINGAATYITLLERARRPHEVSLE
                     LPPAWSRSVSALSATNGGRANHYRAPDFDTLVDSPIVAGNPAVHAFTVGGVPHALVNV
                     GEAGVWDTARSVRDVARIVEAAKEFWSTLPYQQFLFVNLLTGGDDGIEHKASTVLIAS
                     RSATGTDSTYAGWLWLVAHEFFHTWNGKRLRPVELGPFDYETEAYTKCLWIVEGLTTY
                     YAWLIPRRAGLISRQQTLADISDAIESLQTTPGRLVQPLELASYDAWIKLYRPDENSP
                     NASISYYTKGSIVGLLLDAHIRRLTGGRRSLDDVMRLAFRRFSGARGYTPAEFRAVAS
                     QIAGADLTDFFHHAVDTTEDLDYTEMLDWYGLRFARRDTSARPAAWLGASTEIKNGRL
                     QVSQVLRDTPAYQAGLSAGDELIALDSARVSTGGLDSLLGQHEPGDRVTLLVSRRGDQ
                     RTLDITLAAAPARRWHLETRHDATAEQTARLEAWLGK"
     gene            2127..2684
                     /locus_tag="VFB89_15120"
     CDS             2127..2684
                     /locus_tag="VFB89_15120"
                     /inference="COORDINATES: protein motif:HMM:NF016087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein NlpE N-terminal
                     domain-containing protein"
                     /protein_id="HZM28678.1"
                     /translation="MVRRLSLLLCRAVLGLTTALSLALTGCGSGGDATAPDPDPGQHD
                     PAPHPDPQPQPQPQPDAGVPGTYGLVLVNGSQAGRMVLLSNPDGGAIGLFRFDAATSL
                     TLSADHTYELTMRLADDKNTYLLHDTGNYEVTPNGEKLSLTFISQGAAGTYPGVSTGN
                     GAVVDYDIDGDGTPETFLGFARIGG"
     gene            2868..>4330
                     /locus_tag="VFB89_15125"
     CDS             2868..>4330
                     /locus_tag="VFB89_15125"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZM28679.1"
                     /translation="MTTLEPSVGRPRYSAWTPLTFSVFRMLWLTVLVSDIGTWMQTVG
                     AQWLLVSVPNAATLVALVQTADTLPDLLLALPGGALADVFDRRRLLIGLQVFQVAAGV
                     GLTGLTLAGQMTPPLLLGFTFVLGTASALATPAYQALIPELVPRDQLSSAAALGGISV
                     NLARAIGPAVAGLVIARLGVGAVFALNTAAFLLFALALLAWRRPAANRLAMPESFIPA
                     LRAGGRYVRYSPILRRILLRLGVFIVPASAIWALLPLIATRLLGLGASGYGLLLGALG
                     TGAIIGALVLPRVRDQLSSNQILMGAAGVYGAAMAALVLVHNPFAALAALVPAGASWI
                     TVIASTSSAAQLALPGWVRARGLAAYQMVLFGSQAVGALMWGLVAEHSNLVVTFLLAA
                     ASLVAGAATLRLWPLFDIDKLRPDRSVHWPEPKLVIDADPDAGPILVTTTYTIAPERE
                     QQFLQAMRAVRLSRLRTGAMWWELYRDGETAHRFIEA"
BASE COUNT          700 a         1463 c         1370 g          797 t
ORIGIN      
        1 ttttcagcgc gagatccgat tcgccgcccg gctgcagcac cacctgaatc aggcgatcga
       61 tcatctctcg cgcgcgatcg agcccgggtg gcgcgacctc gcgatgctgg accacggatc
      121 cgagttccag cggtaccaga gcgaccctgt actacgcgag ctgcggaccg gagtcgaggc
      181 gctgcccaag gtgccggtgc ccgcagcgat cgaaggcctc gcgagtcggc tcggggatgc
      241 ttagacgccg cctcgctgta tgcctgtctg cggtggcggt cgcggggctg tccggtgttt
      301 ctcttaaggc gcagaagctc gagccggtcc gctacatact ccggatcccg gccccacaaa
      361 cccattacat cgaggtcgaa gcaacctatc ccacatcggg ccggccgacc gtcgacctga
      421 tgatggcggt ctggacgccg ggctcatacc tgatccgcga gttcgagcgt cacgtggaga
      481 gtatgcgcgc acgggacact gctgaccgtg cgctggaact ggagaaaacc cgcaagaatc
      541 gatggcgagt ctacaccggc ggcgctcgga ccatccggct gaactaccgg gtgtacgcca
      601 acgagcatca ggcgagaacc gactgggtgg aggattcctt tgccctgatc aatggcgccg
      661 ccacgtatat cacgctgctc gagcgcgccc gccgcccgca cgaggtctcg ctcgagttgc
      721 cgccggcctg gtccaggtcc gtctcggcgc tctcagcgac gaacggaggc cgcgcgaatc
      781 actatcgggc tcccgatttc gacaccctgg tcgattcccc catcgtcgcc ggaaatccgg
      841 cggttcatgc gttcacggtt ggcggcgtgc cgcacgcgct cgtgaacgtg ggcgaggcgg
      901 gtgtctggga cactgcgcga tcggtgcggg acgttgcgcg catcgtggag gcagcgaagg
      961 aattctggag cactcttccc taccagcagt ttctctttgt caatctcctc accggcggcg
     1021 atgacgggat cgaacacaag gcttctacag tgctcatcgc aagtcgcagc gccacaggca
     1081 cagactcgac ctatgccggc tggctctggc tcgtggccca cgaattcttt cacacctgga
     1141 acggcaagcg actgcggccc gttgaactgg ggcccttcga ctacgagact gaggcctaca
     1201 ctaagtgtct ctggatcgtg gagggcctga ccacttacta cgcctggctc attccccgcc
     1261 gcgcgggtct gatatcccgg cagcagacgc tggccgacat ctccgatgcg atcgagtcgc
     1321 tgcaaacgac tccaggtcgc ctggtgcagc ccctcgagct ggcatcctac gacgcctgga
     1381 tcaagctgta tcgcccggac gagaattcgc cgaatgcctc catcagctac tacaccaaag
     1441 gctccatcgt agggctcctg ctcgatgccc acatccgccg cctgaccggc gggcggcgga
     1501 gtctcgacga tgtcatgcgg ctcgccttca ggagattcag cggcgcacgt ggatacacgc
     1561 cggcagagtt cagggcagtc gccagtcaga tcgcaggcgc cgacctcacc gacttctttc
     1621 accacgccgt cgatacgacg gaggacctgg actacaccga aatgctcgac tggtacggac
     1681 tccggttcgc gcgccgcgat acgagcgccc gtcccgccgc atggcttggc gcgagcacag
     1741 aaatcaaaaa cgggcgactt caagtctcac aggtgctccg cgacacgccg gcctaccagg
     1801 ccgggctctc cgccggcgac gagctgatcg cactcgacag cgcgcgggtg tcgaccgggg
     1861 ggctggattc gctgctgggc caacacgagc caggcgacag agtcaccctg ctcgtttcgc
     1921 gccgaggcga tcagcgtact ctcgatatca ccctggctgc cgctccggcg cggcggtggc
     1981 atctggagac tcggcacgac gcgaccgcgg agcagactgc ccgcctcgag gcctggctcg
     2041 gcaagtaaca acccggagtg agattcggaa gcgccacgct gcgtacctcc ctctgagcga
     2101 aagcaacatt cactcggagg agtcacatgg tacgccgtct ttctcttctg ctctgtcgtg
     2161 ccgtactcgg cctgacgacc gcgctttcgc tcgccctgac gggctgcgga agcggcggcg
     2221 acgccaccgc accggatccc gatcccggcc agcacgatcc ggcgccgcat cccgatccgc
     2281 agcctcagcc ccagccgcaa cccgatgccg gggttccggg cacgtacggc ctggtgctgg
     2341 tcaacggctc ccaggctggg cggatggtcc tgctcagcaa ccccgacgga ggggcgatcg
     2401 gtctttttcg tttcgacgcg gctacttctc tcacgctctc ggcggatcac acgtacgaac
     2461 tgaccatgag gctggccgac gataagaaca cctacctgct ccatgacacc ggtaattacg
     2521 aggtgacccc caacggcgag aagctgtcgc tgaccttcat ctcgcagggc gccgcgggga
     2581 cctaccctgg cgtgagcacg ggtaacggag ctgtggtcga ctacgatatc gacggcgacg
     2641 gcactcccga gacgttcctc ggtttcgccc gaatcggcgg ctgacatccg ggtgaaagca
     2701 cgaagccggc cgcaggtcac tgcggccggc tttgttcctg cgctgaccac ctcgggctga
     2761 gccacacgcg ttccggttcg ccagccaact gtctattgta cggctccggt ctccgccagg
     2821 cagcctcggc acgtaacgtt gcattgaaac gaggatcggg ctaacccgtg accactctcg
     2881 agccaagcgt ggggcgacca agatactccg cttggacgcc gctcaccttt tccgtgttcc
     2941 ggatgctgtg gctgacggtg ctggtcagcg acatcggaac ctggatgcaa acggtcggcg
     3001 cccagtggct cctggtcagt gtgccgaacg ccgccaccct cgttgccctg gttcagaccg
     3061 ccgacacctt gccggacctg ttgctcgctc tacccggagg cgcgctcgcc gatgtcttcg
     3121 atcggcgccg cctgctcatc ggcttgcaag tgtttcaggt cgcggccggc gtcggactga
     3181 cggggctgac cttggccggg cagatgactc cgcccttgtt gttggggttc accttcgtgc
     3241 tcggcacggc atcggcactc gccaccccag cctatcaggc cctcatcccg gagttggtcc
     3301 cccgggacca gctttcgtcc gctgccgcgc tcggcggtat cagcgtcaac cttgcccgcg
     3361 cgatcggccc tgccgtcgcg gggctcgtga ttgcccggct cggtgtcgga gcggtgttcg
     3421 ctctcaacac ggcagcgttc ctgctgttcg ccttggccct gctggcgtgg cgtcgcccgg
     3481 ccgcgaatag gctggcaatg cccgagagct tcatcccggc gctgcgcgcc ggtgggcgct
     3541 acgtgcgcta ctcaccgatc ctccggcgaa tacttctgcg gcttggtgtg ttcatcgtac
     3601 cggcgagcgc catctgggca ttgctgccgt tgatcgccac ccgcctgctt ggacttgggg
     3661 cgagtggcta cggcttgtta ctcggggcgc tgggaaccgg cgcgattatc ggggctctcg
     3721 tccttccaag agtccgcgat cagctctcga gcaaccagat attgatggga gctgcgggcg
     3781 tgtacggggc agcgatggct gcgctcgtgc tggtgcacaa cccctttgcc gcgctggctg
     3841 ctctggtgcc agccggcgcc tcgtggatca cggtcatcgc gagcaccagc tcggcggcgc
     3901 agctggccct accgggctgg gtgcgcgccc gaggtctcgc agcgtaccag atggtcttgt
     3961 tcgggtctca ggccgtcggc gcgttgatgt gggggctcgt tgcggagcac agcaacctcg
     4021 tcgtcacgtt tctcctcgct gcggcgtccc tcgtggcggg agctgcaacg ttgcgtcttt
     4081 ggcccttgtt cgacatcgac aagctcaggc cggaccggtc agtccattgg cccgaaccca
     4141 agctggtgat cgacgccgat cccgatgcag gaccgatact ggtgacgacc acctacacca
     4201 tcgcgcccga gcgagagcag cagttcttgc aggctatgcg cgcggttcgg ctctcgcggt
     4261 tgcggaccgg ggcgatgtgg tgggaactgt accgggacgg cgaaacggcg caccgtttca
     4321 tcgaggcgta
//
LOCUS       DASRJQ010000667         4460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1247498, whole genome shotgun sequence.
ACCESSION   DASRJQ010000667 DASRJQ010000000
VERSION     DASRJQ010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247498"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2416
                     /locus_tag="VFB89_15130"
     CDS             <1..2416
                     /locus_tag="VFB89_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28680.1"
                     /translation="GGHSSRAEAHEPISRQPVLSFPEPGLDDRAAYQGYKTRFYRDSR
                     NNTVQIYVEPKGTRVVLVWADAANESVGFTVRDASGKPARLDWAGEEAQVTDSGPARV
                     IEYQLTASTPSVQLGWFLLGSMRVERDFQYDRRHLRPYARFPFRVAEESLLVATLERL
                     PEEERGRQLEILAADGMSTLRQRLNPSLAIERADTGWSARIERPSLDGKNRLVLEFRG
                     DAQASIAHISGSTVVVKSRTKAPVRLGVRVTTDAAALSPLSRTDIFSSAFLKFLARAR
                     DAGDPGYRRLEREVRGVELLSSKEKLMAGLPNFATYFGRDMMVTALMMRPIWSPAMSE
                     HVIASVLRKLGPAGDVSHEEALAGQAIRENAGIYDSLVSRSLRAGRDGSQERADSLMA
                     EARNVLAHLQATRENYYMRDDEFQLPVLAARYLGDTAVSAERKRGFLLDSADGVARLT
                     LLMRELELVGNETRAYSRDPRPTNLVSFPRRDSTHWRSASWRDSDAGYAGGRFAMDIN
                     AIWAGEALRGVATILAILPRIGIGSATLARLSPKMSDGVMGEYSRDSSALRRAIEVWG
                     DARRHFQVVLGPSEIQRQIRAKLAWMPPTEQRYWQGVLASLGPVRDSLAFLALSLDTD
                     GRPIPVVNTDPATELFLASSGAAQPDSAKRAVLVRDLAPLLRPYPIGLFVAGLGPLVA
                     NDVYASRGVWERFRQDLYHSPRVVWGREVNLLFLGLANQIAGLTSQPSADGDLSMYLH
                     AIDAALDRSLTAVKQSGLEHNELWSYRIANNRLRPIRYGTSSDVQLWNTTDLVVQFVL
                     SGLPPP"
     gene            2590..3204
                     /locus_tag="VFB89_15135"
     CDS             2590..3204
                     /locus_tag="VFB89_15135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28681.1"
                     /translation="MRFVSQCMTLSAAGLLALGAASGASAQQQQPRDSSIESGAVTGA
                     DTASTPTVSSPSMTTDTGSTVNSGNTSQPSSDTGSTSVGSPSTTNNPPSVSSPSSVSS
                     DTGMTGMVNDTSKSRTSTDTALKAKPGVQTGPAAGDTGGVSATTDTSTTADTSKAAHK
                     QHHKKGAKGYKYTGAPSDTTLHAKPGTQTGKSTDSASARPDSTH"
     gene            3301..3558
                     /locus_tag="VFB89_15140"
     CDS             3301..3558
                     /locus_tag="VFB89_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28682.1"
                     /translation="MAQLKKSADAAVKRVVETAEQAQDGLNEVAEEARTRVKRIAGRI
                     SGRSKRRRKVRIAAAAVGIVLAVAAGIAVSRFLTGESQRWR"
     gene            3588..>4460
                     /locus_tag="VFB89_15145"
     CDS             3588..>4460
                     /locus_tag="VFB89_15145"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZM28683.1"
                     /translation="MAESIRDLLFGGPMARGLVETITPRRTFADVVLPPETIAALDYA
                     LQVIRKHDLLFREWGLGERHATGLGLAFHFAGPPGTGKTICAEALAHALGRQLLVVRY
                     SELESRWVGQTGKHVATVFRAAERQGAVLFFDEADAIAGRRFTMATVGYEREANAVVD
                     VLLREIERFDGIVIFATNLAASMDPAFERRIGTHILFRLPGVEERERIWRVQIHPQKT
                     PLADDVDFRVLAEEFPRSGGDIRNAVLKAAQLATLDPVPDSEKRIHRRHFEQAMQDVI
                     AASEVMAQSLFDEAG"
BASE COUNT          836 a         1389 c         1451 g          784 t
ORIGIN      
        1 gggcggccat tcgtcacggg ccgaagcaca cgagccgatc tcgcgtcagc ccgttctctc
       61 attccccgaa cccggtctcg acgaccgggc cgcataccag ggatacaaga cccgctttta
      121 ccgggactcc aggaacaaca ccgtccagat ctacgtagag ccgaaaggca cccgagtagt
      181 actcgtgtgg gctgatgcgg cgaatgagag cgtcgggttc accgtccggg atgcgtccgg
      241 aaagcccgct cggctcgatt gggccggcga ggaagctcaa gtcaccgact cgggtccagc
      301 cagggtaatc gagtatcagc tgacggcttc cactccgtcg gttcagctcg gctggttcct
      361 gctcggctcg atgcgggtgg aacgcgattt tcaatacgac aggcggcacc tccgccccta
      421 tgcgcggttt ccctttcgag tagccgaaga gtcgctgctc gtcgcgacgc tggagcgcct
      481 tcccgaggag gagcgcgggc ggcagctcga gatcctcgcc gccgacggca tgtcgacgtt
      541 acgacaaagg ctcaacccca gcctggctat cgaacgcgca gataccgggt ggagcgcgag
      601 gatcgagcgg ccatcgctcg acggcaaaaa ccggctggtc ctcgagtttc gcggcgacgc
      661 ccaggcttcg atcgcgcaca tctccggaag cacagtggtg gtgaagtccc gtacgaaagc
      721 gccggtgcgc ctcggagttc gggtgaccac ggacgccgcg gcgctcagtc cactctcgcg
      781 caccgacatc ttcagctctg ccttcctgaa gtttctggct cgcgccaggg atgccggcga
      841 cccggggtat cggcgtctcg agcgcgaggt ccggggagtg gagctgctca gcagtaagga
      901 aaagctgatg gcgggccttc ccaacttcgc cacgtatttc ggccgcgaca tgatggtgac
      961 tgcgctcatg atgcgcccga tctggtcgcc ggccatgtcc gagcacgtga tcgcgagcgt
     1021 gctgcggaag ttggggcccg ccggcgacgt gagccacgaa gaagccctgg cgggccaggc
     1081 gatccgggaa aacgccggga tttacgattc gcttgtctcc cggtctctcc gtgccgggcg
     1141 ggatggttcg caggaacggg cggatagtct catggctgag gcgaggaacg tgctcgccca
     1201 cctgcaggcg acccgcgaga actactacat gcgggacgac gagtttcagc tgccggtgct
     1261 ggcggcgcgc tatctgggag acacagccgt cagtgccgag cgcaagcgcg ggttccttct
     1321 cgattctgct gacggagttg ctcgactcac cctgctgatg cgggagctcg agctggtcgg
     1381 gaacgagact cgtgcctaca gtcgggatcc gcgcccaacc aacctggtca gcttcccccg
     1441 ccgggactcg acccattggc gctcggcaag ctggcgtgac agcgacgctg gatatgcggg
     1501 tgggcgcttt gcgatggaca tcaacgccat ctgggccggc gaggcgttgc gtggcgttgc
     1561 gaccatcctg gcaatactgc cgcgaattgg aatcggttcc gccacgctcg cccgcctgag
     1621 tccgaagatg agcgatggcg tcatgggcga atactccagg gacagctccg cgctgcgacg
     1681 agcgatcgaa gtttggggcg acgcgcgacg ccatttccag gtagtgctcg gtccttcgga
     1741 gatccagcga caaatccggg caaagctggc ctggatgcct ccgaccgagc agcgttattg
     1801 gcaaggcgtt ctggcgtctc tagggccggt ccgggattcg ctggccttcc ttgcgctgtc
     1861 cctcgacacc gacggacggc ccatcccggt ggtcaacacc gacccggcaa ccgaactgtt
     1921 tctcgcaagc tccggcgcag cgcaaccgga tagtgcgaag cgcgctgtcc tggtgcgcga
     1981 tttagctcca ctgttgcgcc cgtatcccat cggactcttc gtcgccgggc tcggaccctt
     2041 ggtggccaac gatgtctatg catcgcgcgg ggtatgggaa cgcttccgcc aggaccttta
     2101 ccactcaccc cgggtcgtct ggggtcggga agtcaacctc ttgttcctgg gcctggctaa
     2161 ccagatcgcg ggcctcactt cccagccgtc ggctgatggc gacctgagca tgtatctcca
     2221 tgccatcgat gcagcgctcg accgatcgct tacggccgtg aagcagtccg gtctggagca
     2281 caacgagctg tggagctatc gaatcgccaa taaccggctg cgacccattc ggtatgggac
     2341 cagctcggat gttcagcttt ggaatacgac cgatttggtc gtgcaattcg tactctccgg
     2401 tctaccaccc ccgtgagcct caagtagcat gtgaggaagc ccactcgttt ttgcactgcg
     2461 aggtcacggc aggggcccat tgatggcggt agggtcacta cggcgcccgc gagagcgggg
     2521 cataagcgaa ggcgacgaca acagcgactc actgcagatt tcgagaacag gcgaaatgga
     2581 ggtttgacca tgcggtttgt ttcccaatgc atgaccctct ccgctgctgg tctactggca
     2641 ctcggagcgg cctccggggc ctcggcacag cagcaacagc ctcgcgattc atccatcgag
     2701 agcggggccg tgaccggcgc cgatacggcc agcacgccga ctgttagttc gccgagcatg
     2761 actacggaca cgggttcgac tgtcaacagc ggtaacacca gccagccgag cagcgatacc
     2821 ggctcgacca gcgtcggaag tccatcgacc acgaacaatc cccccagcgt cagcagcccc
     2881 tcgagcgtaa gcagcgatac tggaatgacc ggtatggtca atgacaccag caagtcgcgc
     2941 acgagcacgg ataccgcgct caaggcgaag ccgggcgtgc agaccggccc agctgcgggt
     3001 gacacgggcg gtgtgagcgc cacgaccgat acttccacga ctgccgacac ttccaaggct
     3061 gcgcataagc agcaccacaa gaagggcgcc aagggctaca agtacaccgg tgcacccagt
     3121 gataccacac tgcacgcgaa gcccggcacc cagaccggca agtcgacgga cagcgccagc
     3181 gcgcgtccgg acagcacgca ttagcaggaa cctgcgagaa cgagcggagc gatgtaatac
     3241 ccccgggcgt gggcccgggg gtatccttta tctaatggtt gcgttacccg gaggatggtt
     3301 atggcgcagt taaagaaatc ggccgacgca gcggtcaagc gcgtggtcga gaccgcggag
     3361 caggcgcagg acgggctcaa cgaagtcgcc gaagaggcca gaacgcgggt caagcggatt
     3421 gcagggcgaa tctccggcag gtcgaaacgg cggcggaaag tgcgcattgc tgccgcagcg
     3481 gtgggaattg tgctggcggt tgcggcaggt atcgccgttt cccggttcct tacaggtgaa
     3541 agtcaacgat ggcgatgacg accgggcctg atccgcacta ggtcgatatg gctgagtcga
     3601 tcagggatct gctgttcggc ggaccgatgg cgcgggggct ggtcgagacg atcacgcccc
     3661 ggcgcacctt cgccgatgtc gtattgcccc ccgaaacgat cgccgcgctg gactacgctc
     3721 ttcaggtcat ccgcaagcac gacctgttgt tccgggaatg gggcctgggc gaacggcatg
     3781 ccaccggatt agggcttgcc ttccatttcg ccggtcctcc cggaaccggg aagacgatct
     3841 gtgcggaagc gctggcccac gctctgggtc gacagctgct cgtggttcga tattccgagc
     3901 tcgagtcacg ctgggtggga cagaccggta aacacgtcgc aaccgtgttt cgtgcggccg
     3961 agcggcaagg cgccgttctc tttttcgacg aggcggacgc gatagccggc cggcgcttca
     4021 ccatggcgac agtcggctat gagcgcgagg cgaacgctgt cgtcgacgtg ctgctgcggg
     4081 agatcgagcg gttcgatggc atcgtcattt ttgccacgaa cctcgccgcc agcatggacc
     4141 ctgcgttcga gcgccgcatc ggtacccaca ttctcttcag gcttcccggc gtggaggagc
     4201 gggagcggat ctggagagtg cagatccatc cacagaagac ccccctggcg gacgacgtgg
     4261 attttcgcgt tctggcagag gagttcccgc ggagtggtgg ggacattcgc aacgcggtgc
     4321 tcaaggccgc acagctcgcc accctggacc cggttccgga ctcagagaag cgtattcacc
     4381 ggcgtcactt cgagcaggcc atgcaagatg tgatcgcagc gagtgaagtg atggcgcagt
     4441 ccttgttcga tgaggcaggc
//
LOCUS       DASRJQ010000668         2640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2702935, whole genome shotgun sequence.
ACCESSION   DASRJQ010000668 DASRJQ010000000
VERSION     DASRJQ010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2702935"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VFB89_15150"
     CDS             <1..777
                     /locus_tag="VFB89_15150"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZM28684.1"
                     /translation="LFTNSASVPVVVRGNPNLDVEHVTSYEIGWKGQFGPRVFLTLDA
                     YYSRLSNFVTDILPAVNPTYGPWTAPAEVAADSRAAVEQAVQDILVGAGQEVAAAGLS
                     RLQDGATAIVFSIGNAGRVSEHGFEATAGLRLTDALSLDLNYSLFRFRVDAASLASGQ
                     VLKPNTSPNAFNVGLAYTTPRLDARAMLHVVEGFDWASGVYVGRIPSRAPIDVSVGWQ
                     AQSWLNVHAAATNLLNQRRYQVFGGSVIGRRALLGVTMVR"
     gene            778..996
                     /locus_tag="VFB89_15155"
     CDS             778..996
                     /locus_tag="VFB89_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28685.1"
                     /translation="MVATGVEATMPGGAQAILATPPSSKAMSRLIDGLAPKGKLMVIG
                     EAIQGYVSGTPTDSEDTRPSPSSFAASR"
     gene            1117..>2640
                     /locus_tag="VFB89_15160"
     CDS             1117..>2640
                     /locus_tag="VFB89_15160"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZM28686.1"
                     /translation="MKLFPPFRLDTVNECLWRSTEAGADERILLTPKSFEVLRYLVEH
                     AGRLVTQRELLEAVWPGTFIEPQAVKKQIFDLRRVLDDPPRTPRFIETLPRRGYRFIA
                     TVEESALRTPAVSPPLTRRLVGRDGALAELRNYLRKAMAGERQIVFITGEPGIGKTAL
                     AEEFQWQTAAREPSVRIARGQCVEGFGSKEPFYPMLEALGELCRGLDGATILEVLATE
                     APTWLVQFSGLLRRDQREALQREVLGATPERMLRELVKALETIASSRPLLLVFEDLHW
                     VDYSTVDLISAVARHRTPAKLMLVATYRPLDVALSDHPLGVLKPNLLARQFCHEIEVP
                     PLSVPEVVQYLATDPSAAEAPSGLAELVHRHCEGNPLFMIGLLEHLSGQGFIKRTERA
                     WELRCPLSSVELEIPESLRQVIETQVQALSPDDQRVLEVASIAGVTFTPLVSAVTGNL
                     DPGNFEERCEALARRQYLLLIADTDALPGGDIAQRYEFQHALYREVLYRRQAPAHRAT
                     "
BASE COUNT          435 a          907 c          831 g          467 t
ORIGIN      
        1 ctcttcacca actcggccag cgtgcccgtc gtggttcgcg gcaatcccaa cctcgacgtg
       61 gagcacgtga cgagctacga gatcggctgg aagggacagt tcggcccccg ggtcttcctg
      121 accctcgacg cctactacag ccgcctgtcg aatttcgtga ccgacattct ccccgccgtc
      181 aacccgacgt acggcccctg gaccgcacct gccgaggtcg cggccgactc tcgggccgcg
      241 gtcgagcagg cggtgcagga catcctcgtc ggggccggcc aggaggtcgc ggcggccggc
      301 ctctcgcgcc tgcaggacgg ggcgacggcg atcgtattct cgatcggcaa cgccggccga
      361 gtcagcgagc atggcttcga ggccacggct ggactccggc tgaccgacgc gctctccctc
      421 gatctcaact actcgctctt ccgtttccgg gtggacgccg cctccctcgc ctcgggacaa
      481 gtcctcaagc cgaacacctc gcccaacgcg ttcaacgtcg ggctggccta caccacgccg
      541 cgactcgatg cccgcgcgat gctgcacgtg gtcgaaggct tcgactgggc ctcgggtgtc
      601 tatgtgggac ggatcccgtc ccgtgcgccg atcgacgtca gtgtgggctg gcaggcgcag
      661 tcgtggctga acgttcacgc agccgccacc aacctcctca accagcgtcg ctaccaggtg
      721 ttcggcgggt cggtgatcgg ccgccgagct ctgctcggcg taaccatggt gcggtaggtg
      781 gtggccacag gcgtcgaggc gaccatgcct gggggtgcac aggccattct ggcgactccc
      841 ccaagctcga aagccatgtc gcgtctgatc gacggtctgg cgcccaaggg caagctcatg
      901 gtgattggtg aggcgattca gggttatgtc tcgggcacac caacggattc cgaggatacc
      961 cggccttcgc cgagctcatt cgctgccagc cgatgacccg cttcggatga catttctctc
     1021 ggcaccgcgc atcttgacga tcctcgccgg cggctagaaa gcgcggctgc tgccggcctg
     1081 cttgcccgga tagcgttcga gtggaggcgc gccttagtga agctgttccc tcccttccgt
     1141 ctcgacaccg tgaacgagtg cctctggcgc tccaccgagg ccggggcgga tgagcgcatt
     1201 ctcttgacgc caaagagctt cgaagtcctc cgctatctgg tggagcacgc cggccgactg
     1261 gtcacccaac gcgagctgct cgaggcggtc tggccgggca ccttcatcga accacaggcc
     1321 gtcaagaagc agatcttcga ccttcgccgg gtactcgatg acccgccgcg gaccccgcgc
     1381 ttcatcgaaa ccctgccgcg ccggggctac cgtttcatcg ccaccgtcga ggaaagcgcg
     1441 cttcgcaccc cggccgtctc gcccccgctg acccgccgcc tggtcgggcg agatggcgcg
     1501 ctggccgagc tccggaacta tctccgaaag gccatggccg gcgagcggca aatcgtcttc
     1561 atcaccgggg agcccggcat cggaaaaact gcactggcag aggagtttca gtggcaaacc
     1621 gcagcccgcg agccatcggt tcggatcgcg cgcgggcaat gcgtcgaggg ctttggcagc
     1681 aaggaaccgt tctacccgat gctcgaagcc ctcggcgagc tgtgccgcgg tctcgacggc
     1741 gccaccatcc tggaggttct cgcgacggaa gcgccaacct ggttggtgca gttcagcggc
     1801 ctgctgcgac gcgaccagcg cgaggcgcta cagcgcgaag tcctcggtgc cacccctgag
     1861 cgcatgcttc gggagctcgt gaaggctttg gagacgattg cgtccagccg cccgctcctg
     1921 ctcgtcttcg aagatctcca ctgggtcgac tattcgacgg tcgatctcat ttcggccgtc
     1981 gcgcggcacc ggacgccagc caaactcatg ctggttgcga cctatcgccc acttgacgtg
     2041 gccctctcgg accatccgct cggcgtgctc aagccgaatc tccttgcccg tcagttctgt
     2101 cacgaaatag aggttccgcc gttgagtgtg ccggaggtgg tccagtacct cgccaccgat
     2161 ccttcggccg cggaggctcc ctcggggctt gccgagctgg tccaccgtca ctgcgaggga
     2221 aatccgctgt tcatgatcgg cctgctcgag cacctgagcg gacaagggtt cataaaacga
     2281 acggagcgcg catgggaact gagatgcccc ctctcaagtg tcgagctgga gatcccggag
     2341 agcctgcgcc aggtgatcga gacccaggtt caggcgctca gtcccgatga tcagcgagtc
     2401 ctcgaggtgg caagtatcgc gggggtgacg ttcactcctt tggtcagcgc ggtcacgggg
     2461 aatctggatc ccgggaactt cgaggaacgg tgcgaggcac ttgcccggcg gcagtacctg
     2521 ctactgatcg cggacaccga cgcgctcccc gggggcgaca tcgcccaacg ctatgaattc
     2581 cagcacgcgc tctatcgcga agtgctgtat cgccgccagg cacccgcgca ccgagccact
//
LOCUS       DASRJQ010000669         4456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1770813, whole genome shotgun sequence.
ACCESSION   DASRJQ010000669 DASRJQ010000000
VERSION     DASRJQ010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1770813"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2770
                     /locus_tag="VFB89_15165"
     CDS             <1..2770
                     /locus_tag="VFB89_15165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HZM28687.1"
                     /translation="PAEEIVGDRQPLSRSYDVFDNPLQTQLPGGRLQPAGAIAGWSRG
                     FDTLDRVTTVGPLGTGAHTSGAAWKWGGAARLFEMTTAGGRGTQARFGYHSGPGPSSS
                     AEPAPWRLGALEWGTSGGATWGKFEAGWRQGDGAKLSRPSAGGPFAGLGWSYRYDAGV
                     RLSSASGDLDRWGFDYGLGDERESERRDQTGELTLFGAGANGRIETRGALAFAHDLSG
                     RRTEDDRFSYVWDWRGQLVEVTVKDIWPDADGDGELDRSPFAGHQVRYRYDAVGRLLQ
                     REHRGIQLTSGDRTLLELREYVWDGETLAAEISWASPDRTNARWRKTYVPGASGLDDS
                     VQVLVEIGDQAGNPFANSTHLYTYLRDETGSVVGMVAEDEAPAEASKPAAPVRYLYTP
                     YGEAHAESGPELRRAHYDNSLISAGGITQDVSNLHAAAPGAMVLDWSLGIDAASLAQG
                     LAVEKLEAGTGWLALSPDKVIMAVESGEGELGRAVVMAVRGWEASTSYRVRIKPELRD
                     TLGRTFGKTESFEWRVPDPPAEASAPLPTIAFDKKIPVRFENWAAAWNDVGGRFPGGQ
                     TALFQGLYTDNVTGLSYARARWYDARNASWLSEDSVLATDSPNFYAFVAWAPSMASDP
                     LGEESIRQWWEIENDLVDRPLWGGTKWFAYQAWNMGTFGFLGEHDELFETTSGSEYAA
                     RTALAAGKAGAKLYVAVQTAGAGAAFGQAVGLGRIATGTLVGGAVGGTLTATDDLFNF
                     AEGKPTHSLRDYATNIAAGAALGALGGTWEQVRSSGMAGPRPTAPRVNLARPRPQTPI
                     EEIPGAVQGGRRVYKIPPGSSGGATAYEKITPAIRERYFPANEPAPLCSYCRYNPARP
                     VDHVWARSKGGNLEPENTTPACDHCNPSKRDYTAPKNPPPGYQGQWPPPWWPTGMRNW
                     WMRTHGGR"
     gene            2757..3233
                     /locus_tag="VFB89_15170"
     CDS             2757..3233
                     /locus_tag="VFB89_15170"
                     /inference="COORDINATES: protein motif:HMM:NF025451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4265 domain-containing protein"
                     /protein_id="HZM28688.1"
                     /translation="MVDDDLIAVHERPVWKERANFLVHIDLHPWGLPGRLEQLWVRRE
                     GERLFELCCIPFFSYGFHLGDVVETTPQNDLEFVVNRVVSPSGRRNIRIAVINPDDAE
                     LITKRLEVECENLCCPYEVCRPGYMAIDLPSPNHERVLLTAINDLLQSRAIMMEEV"
     gene            complement(3353..4126)
                     /locus_tag="VFB89_15175"
     CDS             complement(3353..4126)
                     /locus_tag="VFB89_15175"
                     /inference="COORDINATES: protein motif:HMM:NF018684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HZM28689.1"
                     /translation="MSTKTAGPKSSMSATFRRREMKLTRKHSHGSMLALFLLTGFALG
                     SSRPARANADEPSATPALDACQRFASHHYQQVSPKDFVSVRLLEEGVNQEKYEGKVGS
                     QLVGSVLSGPGIWQGKTGGPSNVHFVCLLENSEKPVFVAVVQNGRRDPVAVCWDAFEP
                     SEWGRMTQCLQDSLKREEAALADALNKAAQQAGQSMDKISAKQTLQESNAQWVKYRDS
                     ECARRQAFVAGRNHPDIGELTCQIGKTAERISDLQFDDN"
     gene            complement(4227..>4456)
                     /locus_tag="VFB89_15180"
     CDS             complement(4227..>4456)
                     /locus_tag="VFB89_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28690.1"
                     /translation="AVQAASLSNAAANGTWSEGILNAAGLAAISKTGTTQLRVYFNLD
                     DNDDTGNDFIGYYSGDNATAANRPQLVVTYQ"
BASE COUNT          792 a         1390 c         1468 g          806 t
ORIGIN      
        1 gccggcggag gagatcgtcg gcgaccgcca gccgctctcg cgcagctacg acgtgtttga
       61 caatccgctg cagacgcagc tccctggcgg ccgtctgcag ccggcaggag cgatcgccgg
      121 ctggtcgcga ggcttcgaca ccctcgaccg cgtgaccacc gtcggtcccc tcggcacggg
      181 cgcgcacaca tcgggcgccg cctggaagtg gggcggcgcg gcacgcctct tcgagatgac
      241 gaccgccggc ggtcgcggca cgcaggcacg tttcggctac cacagcggtc cggggccatc
      301 ttcgtcggcc gagcccgccc cctggcggct gggagcgttg gaatggggga cgagcggcgg
      361 agccacttgg ggcaagttcg aagcgggctg gcggcagggc gacggcgcga agctcagccg
      421 cccgagcgcc ggcggtccgt tcgcgggcct cggctggagc tatcgctacg acgccggcgt
      481 gcgtctgtcc tccgccagcg gtgatctcga ccgctggggc ttcgactacg gcctcggcga
      541 cgagcgcgaa tcggagcggc gcgatcagac cggcgagctg accctgttcg gtgccggcgc
      601 caacggccgc atcgagactc gcggcgcgtt ggcgttcgcc catgatctct ccggccgccg
      661 cacggaagat gaccggttct cctatgtctg ggactggcga gggcagctcg tcgaagtgac
      721 ggtgaaggac atctggccgg acgcggacgg cgacggcgag ttggaccgtt cgccctttgc
      781 gggccaccag gtccgttacc gctacgacgc tgtcggccgg ctgctccagc gggagcatcg
      841 cggtatacag ctgacgagcg gcgatcgaac cctgctcgag cttcgcgagt acgtctggga
      901 cggcgagacg ctggccgcgg agatctcctg ggcctctccc gaccgcacca acgcccgctg
      961 gcgcaagacc tacgttccag gcgcgagcgg tctcgacgat tcggtgcagg tgctggtgga
     1021 gatcggggat caggcgggga atcccttcgc caactcgacg cacctctaca cctacctgcg
     1081 cgacgagacg ggctcggtcg tgggcatggt ggccgaggac gaagcgccgg cggaggcctc
     1141 gaagccggcg gccccggtgc gctatctcta caccccgtac ggcgaagcgc acgccgagag
     1201 cgggccggag ctgcggcggg cccattacga caactcgctg atctcggcgg gcgggatcac
     1261 gcaggacgta tccaacttac acgccgcggc ccccggcgcg atggtgctcg actggagcct
     1321 cgggatcgac gcggcgagtc tcgcacaggg tctggcggtc gagaagctcg aggccggcac
     1381 cggctggctg gccctgtctc ccgacaaggt gatcatggcg gtggagagcg gggagggcga
     1441 gctcggacgg gccgtggtga tggcggtgcg cggctgggag gcgagcacca gctaccgcgt
     1501 gcgcatcaag ccggagttgc gcgacaccct cggccggacc ttcggcaaga ccgagagctt
     1561 cgaatggagg gtgcccgacc cgcccgcgga ggcgagcgcg ccgctgccga ccatcgcgtt
     1621 cgacaagaag atccccgtcc gcttcgagaa ctgggctgcg gcctggaacg acgtcggcgg
     1681 aaggttcccg ggtgggcaga ctgccctctt ccaggggctc tacaccgaca atgtcacggg
     1741 cctgagctac gcccgggcgc gatggtacga cgcgcggaat gcgtcttggc tttctgaaga
     1801 ctccgtgctc gccaccgata gtccaaactt ctacgcgttt gtcgcatggg ctccgagcat
     1861 ggccagcgac ccgctaggcg aagagagcat tcgccaatgg tgggaaatcg agaacgatct
     1921 cgtggaccgg ccgctctggg gtgggacgaa gtggttcgcc taccaggcct ggaacatggg
     1981 caccttcgga ttcctcggtg aacacgatga actgttcgag accaccagtg ggagcgagta
     2041 cgcagcgcga accgcacttg ccgcggggaa ggcgggggcc aagctctacg tggcggtgca
     2101 aaccgctggg gcaggtgctg catttgggca agccgtcggg cttggcagaa ttgccacggg
     2161 gacgctcgtc ggtggtgcgg ttggaggcac cctaacggca acggatgact tgttcaactt
     2221 cgccgaggga aagcccacac actcacttcg cgattacgcg accaatattg ctgcgggtgc
     2281 agcgttgggc gctctgggag gcacgtggga gcaggtccgg tcgtctggaa tggccgggcc
     2341 aaggccaaca gcgccgagag tgaatctggc gcgccctcga ccgcagacgc ccatagagga
     2401 gattcctgga gccgtgcagg gcgggcgtag agtctataag attccgcccg gttcttccgg
     2461 cggggccact gcgtatgaga aaatcacgcc tgccattcgc gagcgctact ttccggcgaa
     2521 tgagccagcg ccgctctgta gctactgccg ctataacccc gcacggccgg tcgatcacgt
     2581 gtgggcacga agcaaagggg ggaatcttga gcccgagaac acaactcctg cttgcgatca
     2641 ctgcaacccc tcgaagaggg attacacggc acccaagaac cctccccctg ggtatcaagg
     2701 tcaatggccg cccccgtggt ggccaacagg catgcgaaat tggtggatga gaactcatgg
     2761 tggacgatga tctaatcgcc gttcatgagc ggcctgtatg gaaagagagg gcgaactttc
     2821 tcgtccatat agatctgcat ccctggggat tacctggtag acttgaacaa ctgtgggtgc
     2881 gtcgagaagg tgaaaggctg ttcgagcttt gctgtattcc tttcttttca tacggctttc
     2941 acttgggtga tgtggtggaa accacgccgc agaacgactt ggaatttgtg gtgaacaggg
     3001 tagtttctcc atccggtcgg aggaatatac gcattgctgt aatcaatccc gacgacgctg
     3061 agctcatcac caaacgcttg gaggtcgaat gcgaaaattt atgctgcccc tacgaggtat
     3121 gcaggccggg ttatatggca attgatctcc cgtcgcccaa ccatgagcga gtgttgctca
     3181 ctgccatcaa tgacttgttg cagtcgaggg cgatcatgat ggaggaggtc taactcgtga
     3241 acccgaaggt cccgcggaga agcgccgccg cccgcctccg gaggttctcc ccccggctct
     3301 ccaactccta gcgccggcgg acgaactccg tgaactgagt aatagccgat gatcagttgt
     3361 cgtcaaattg taggtcagag attcgttcgg cagtttttcc aatctggcag gtcagctcgc
     3421 caatatccgg gtggttgcgg cctgccacaa aggcctgcct gcgagcgcac tcgctatccc
     3481 gatacttgac ccactgggca ttgctctcct gcaaggtctg ctttgccgaa atcttgtcca
     3541 tcgattgtcc tgcctgttga gcggccttgt tcagtgcatc cgccaaggca gcctcttccc
     3601 gtttcaggga atcctgaagg cactgggtca tccggcccca ctcgctgggc tcaaatgcat
     3661 cccagcacac agccaccgga tctcgccgcc cgttctggac aacggcgacg aagacgggtt
     3721 tctcggagtt ttccagcaag cagacaaagt ggacgttcga aggtcccccg gtcttgccct
     3781 gccaaatacc agggccggac aacaccgagc cgacaagctg gctgcccacc ttgccctcgt
     3841 acttctcctg attcacgccc tcttccagca atcgaacaga aacgaaatcc ttcgggctga
     3901 cctgctgata gtgatggctt gcaaagcgct ggcaggcgtc aagggctggg gtcgcggagg
     3961 gctcatcggc attcgctctt gccgggcggc tgctcccaag cgcaaatccc gtcagcaaga
     4021 acagcgcaag catcgagccg tgagagtgct tcctggtcag tttcatctct ctcctccgga
     4081 aggtagctga cattgaggat ttggggccag cggtctttgt gctcatgcta cgcctctggc
     4141 ttatgctcgt caacgcgacc ttctcccgga cagggggacg gggcgccgag gccccgtccc
     4201 tccttccctg gtccagcgcg tgatcgctac tggtacgtca ccacgagctg cggacggttg
     4261 gcggccgtgg cgttgtcgcc cgagtagtag ccgatgaagt cgttgcccgt gtcgtcgttg
     4321 tcgtcgagat tgaagtagac gcggagctgc gtcgtccccg tcttgctgat cgccgcgaga
     4381 cccgcggcgt tcaggatgcc ttcggaccac gtgccgttcg cggccgcatt gctcaggctc
     4441 gccgcctgca ccgcgg
//
LOCUS       DASRJQ010000670         4373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2377876, whole genome shotgun sequence.
ACCESSION   DASRJQ010000670 DASRJQ010000000
VERSION     DASRJQ010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2377876"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VFB89_15185"
     CDS             <1..304
                     /locus_tag="VFB89_15185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZM28691.1"
                     /translation="AELLILSLLEAEARHGYQLSRLIEARSRGTIRFNVASLYPLLYR
                     LESKGWVQGRWVETAGERRRRFYRLTPAGRKALAAHRRVWADFIKAVGRIVRPEYA"
     gene            297..1967
                     /locus_tag="VFB89_15190"
     CDS             297..1967
                     /locus_tag="VFB89_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28692.1"
                     /translation="MPDWSQRASGVLLPAILAGFAPTEIPTAPATPNSGPATRGTTRL
                     DKRFTAMLDSQRSRGIVPLRLQAAIGSAAMLVAVPFTSMRLIPVEPKAPPITLSEPAP
                     LALVAPVVMTTGKPVVPKPRQAKPTQSTPSFQQADTIVDRTVAASPGETISINLRSVG
                     GVTIRSWNQSQVRVRAILSGALARQTEVLMARVGGGVEFRTLTPPEYRGDWIDRNSFE
                     IWVPTRFNVDISSPGGGISISGVEGRFSGNSAGGGITLDHVSGRADLSTGGGEVRVTN
                     SNLEGSVSTGGGRAVVHNTSGRVSVTSGSGPVIRDGGSGVPSMTLAASSVSATAYQER
                     DEINGSFTLPARARVAVVAINGSVDIKASDGDTASVEIERAARSRAELDCNRVVSRQV
                     AGNLFIKSEAVCENRVIQVRHRVVLSLPRNVDLTVMGVSGPVNVGGIEGTMSISGNSG
                     DINLAQSGRRSRISGNTGTTTITLRQLYAGGLELSGNGGPIKLYIGDELNALVRVSGI
                     SGSVSSDLPDVTFHKTAAADYYARIGSGWPTIYVLGHGGDISLRQYRE"
     gene            2086..2907
                     /locus_tag="VFB89_15195"
     CDS             2086..2907
                     /locus_tag="VFB89_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28693.1"
                     /translation="MSRRLDTKLLKPSIASVLGMLLVLVAFADASGQAASTGQTGVEQ
                     IRTLQLDAWTRPGVGQFVPADDIAQYLVYELYVTNWNGPPPLRFAAVDVEDVATGKRL
                     ARLDTVALETPSTLRITPFPGRAGPANRQLPAGRTAILRIELKLPLGASVPAAVRHRI
                     AFESNPALQLIQDDGSLTSDLVAYTEPMRIDRSPPVVIGPPLLGGPWYSGPSMIRSRS
                     SIRWSSRRAFTSRESPVQSSSRQGRLGRRTSKSNEAPARNGSSTALRPRSITAPI"
     gene            2922..3068
                     /locus_tag="VFB89_15200"
     CDS             2922..3068
                     /locus_tag="VFB89_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28694.1"
                     /translation="MQFTDRPGCGSLDSRHALALEVPSDAFLELSNIYYATGRRDVYL
                     TQAH"
     gene            complement(3176..>4373)
                     /locus_tag="VFB89_15205"
     CDS             complement(3176..>4373)
                     /locus_tag="VFB89_15205"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZM28695.1"
                     /translation="PGDPVARSQAMSRHATLVLLAALIGSAGPALAQQAGQSEFREGE
                     RYRVGAGVPSDVIEARAWYLKAAAKGHPMAQYQLGMIYNYGRGVPKDAAQAISWYKKA
                     AAQGDASAALELGEIYRFGRGVPADWNLAFPWMKQAADAGEMLAILQLAKAYRYGWGT
                     TKDLAKARELFRIGADSGFSTAQYDLGVMYYSGEGMPANEREAVAWFRKSAAGQYGAG
                     PAMRSLGFVYMNGRGGVGRDSAAGVDWYVKAAENGDAPAQNYIGWLYETGTWTTKDLA
                     QAEAWYRKAAQNDDPDGQFNLGRLLMYGIGREKDRYWGASWFQKAAEQGHTAAQFHLS
                     YSYFNGAGVLTDSVAGYKWLYLASTRDPKNQEYRTRLSEVDARVRGDLVARGTALALE
                     WLKRRG"
BASE COUNT          731 a         1501 c         1372 g          769 t
ORIGIN      
        1 cgccgagctg ctcatcctct ccctcctcga ggccgaagcc cgccacggct accagctctc
       61 ccggctgatc gaggcccgct cccggggcac catccgcttc aacgtcgcgt ccctctatcc
      121 cctgctctac cgcctggaga gcaagggctg ggtgcagggg cgctgggtcg agaccgcggg
      181 ggaacggcgc cggcgcttct accggctcac cccggcggga cggaaggccc tggcggcgca
      241 ccggcgggtc tgggccgact tcatcaaggc ggttggccgc atcgtgaggc ccgagtatgc
      301 ctgactggag ccagcgtgcg tccggggtcc tgctcccggc catcctcgcg ggattcgctc
      361 ccacggagat acctaccgcc ccggcaacgc cgaacagtgg tcctgccact cgaggcacca
      421 cacgactcga caagaggttc actgccatgc tggactccca acgctcgcgc ggcatcgtgc
      481 cgcttcgcct gcaggctgcc attggctcgg cggcaatgct cgtcgccgtg cccttcacgt
      541 cgatgcggct gattcccgtc gagcccaagg ccccgccgat cacgctgtcg gagcccgcgc
      601 cgctcgcgct cgtcgctccg gtggtcatga cgacgggtaa acccgtcgtg ccgaaacccc
      661 ggcaagccaa gcccacccag tccaccccgt cattccagca agccgacacg atcgtcgacc
      721 gcacggtcgc cgcgtcgccc ggcgagacga tttccatcaa tcttcgcagc gtcggcgggg
      781 tcacgatccg atcatggaat cagtctcagg tccgggtgcg cgccattctc agcggggcgc
      841 tggcgcgaca gacggaagtc ctgatggcgc gcgtgggcgg cggagtcgag ttccgcaccc
      901 tcacgccgcc cgagtaccgc ggcgactgga tcgaccggaa cagcttcgag atctgggtac
      961 caacgcgttt caacgtcgac atttcgtccc ccgggggtgg gatcagcatc agcggcgtcg
     1021 aggggcgctt cagcggcaac agcgccggcg gcggaatcac gctcgaccac gtctccgggc
     1081 gcgccgacct cagcacgggc ggtggcgaag tgagggtcac gaactcgaat ctcgagggca
     1141 gtgtttcaac cggcggcggc cgagccgtgg tgcacaacac ctccggccgg gtctcggtga
     1201 cctccggaag cggcccggtc atccgcgatg gcgggagcgg cgtgccctcg atgaccctgg
     1261 ccgcatcgtc cgtatcggcg accgcctatc aagagcgcga tgaaatcaat ggaagcttta
     1321 ctctcccagc tagagcgcgg gttgcagtgg ttgccatcaa cggcagcgtt gacatcaagg
     1381 ctagtgatgg cgacacggca agcgtcgaga tcgaacgcgc tgcgcgcagc cgcgccgagc
     1441 tggattgcaa cagagtcgtt tcgcggcagg tcgctggcaa tttattcata aagagcgaag
     1501 cggtgtgcga gaaccgggta atccaagtca ggcatcgcgt ggtgttgagt ctcccacgca
     1561 atgtcgatct taccgttatg ggcgtcagcg gcccggtcaa cgttggaggg atcgagggca
     1621 cgatgagcat cagcggcaat agcggggaca tcaatctcgc acagtctggg cgtcgttcca
     1681 ggatcagcgg caacacgggg acgacaacca ttacgctgcg gcaactctat gcagggggac
     1741 tggagttgag tggtaatggc gggcccatca agctgtacat cggcgacgaa ttgaatgccc
     1801 ttgtgagagt gagcggaatc agcggctcgg tctccagtga cctgccggac gtgacatttc
     1861 acaagaccgc cgccgccgac tactacgcgc gcattgggtc tggctggccg acgatttatg
     1921 tcctaggtca cgggggcgat atctcgctta ggcagtatcg ggagtaattc cgcgtctggc
     1981 gtgattcggc gtcagtaagc atcgtgtcag cattctctct tgcggtcatg agatcacatg
     2041 cgcagatctg tccgttggct cgcagtccct tgaaaggaca ccacgatgag tagacgcctg
     2101 gacacgaagc tactcaagcc atcgatcgca tccgtccttg gaatgcttct ggtgcttgtc
     2161 gccttcgcag atgcgtcggg acaagccgcc agcacgggtc agaccggagt cgaacagata
     2221 cgaacgcttc agctcgacgc gtggacgagg ccgggcgtcg gtcaattcgt gccggctgac
     2281 gacatcgcgc agtatctggt ctatgagctt tatgtgacga actggaacgg tcctccgccg
     2341 cttcgcttcg cggcggtgga cgtagaggac gtcgccaccg gaaagcgtct ggcccggctc
     2401 gacaccgtgg cgctcgagac gccctccacg ctccgcatca ccccgtttcc cggcagagcc
     2461 ggcccggcca accgccagct tcccgcgggc cggacggcga tcctcaggat cgaattgaag
     2521 ctgccgttag gcgccagcgt gccggccgcc gtgcggcatc gaatcgcgtt cgagtccaat
     2581 cccgccctcc agttgatcca ggacgatggc tcgctgacct ccgacctcgt cgcctacacc
     2641 gaaccgatgc ggatcgatcg ttcgcccccc gtggtcatcg gtcctccgct cctgggcggc
     2701 ccgtggtact ctggcccgtc aatgatacgc tctcgcagca gtatccgctg gagcagtcgc
     2761 cgcgcattca catcaaggga gtcaccggtc caatcgtcat ctcgtcaggg ccgactagga
     2821 cggcgaacat caaagtccaa cgaagcgccg gcacgcaacg ggagctcgac tgctctgaga
     2881 ccaaggtcga tcaccgcccc gatttgatca cgatctcgta tgtgcagttc accgatcgcc
     2941 cggggtgcgg atccctggac tcccgtcacg cgctcgcgct cgaggtgcct tccgatgcgt
     3001 tcctcgagct gtccaacatt tactacgcga cgggccggcg ggacgtttat ctcactcaag
     3061 cgcattgatg gcgatatcgt gctcaagcgc cagtgagctg ctctcgcctc gagagttcgt
     3121 gcgcgttcag cgcggcacga cgaagtagcc cgctcaatga agacgtccac cgtggctacc
     3181 cccgccgctt gagccactcg agcgccagcg ccgtcccgcg tgccaccagg tcaccgcgca
     3241 cgcgggcgtc tacctccgac agccgggtcc gatactcctg gttcttcggg tctcgagtgc
     3301 tggccaggta cagccacttg taccccgcca ctgaatcggt caaaacgccc gcgccgttga
     3361 agtagctgta cgagaggtgg aactgggcgg ccgtatggcc ctgctcggcg gccttctgga
     3421 accagctcgc cccccagtac ctgtccttct ctctcccgat gccgtacatc agcagccgcc
     3481 cgaggttgaa ctggccgtcg ggatcatcgt tctgggcggc cttgcggtac cacgcctcgg
     3541 cctgggccag gtccttggtc gtccaggtac ccgtctcgta gagccacccg atgtagttct
     3601 gggccggcgc gtcgccgttc tccgccgcct tgacgtacca atccacgcct gccgcggaat
     3661 cgcgtccaac gccgccgcgt ccgttcatgt agacgaaacc gagcgagcgc atcgcgggcc
     3721 ccgccccgta ctggccggcc gcgctcttcc ggaaccaggc gaccgcttcc cgctcgttgg
     3781 cgggcatccc ctcgccggag tagtacatga cgccaaggtc gtactgtgcc gtgctgaatc
     3841 ccgaatcggc cccgatccgg aacagctcgc gggccttggc caggtccttg gtggtccccc
     3901 acccgtagcg gtatgccttc gccagctgga ggatcgccag catctcgccc gcgtcggcag
     3961 cctgcttcat ccagggaaac gccaggttcc agtccgcggg caccccgcgc ccgaaccggt
     4021 agatctcgcc cagctcgagc gcggcggacg catcgccctg ggcggccgcc ttcttgtacc
     4081 aggagatcgc ctgcgcggca tccttgggca ctccgcgccc gtagttgtag atcatgccga
     4141 gctggtactg cgccatggga tggcccttcg cggcggcctt gaggtaccat gcgcgcgcct
     4201 cgatcacgtc gctcgggacc cccgcgccga cccggtaacg ctccccttcc ctgaactccg
     4261 actggccggc ctgctgggcg agcgcgggac ccgccgagcc gatgagcgcg gcgagcagga
     4321 cgagcgtggc atggcgtgac atggcctggc tccgggcgac ggggtctcct ggc
//
LOCUS       DASRJQ010000671         3042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_4155754, whole genome shotgun sequence.
ACCESSION   DASRJQ010000671 DASRJQ010000000
VERSION     DASRJQ010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4155754"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..1111)
                     /locus_tag="VFB89_15210"
     CDS             complement(71..1111)
                     /locus_tag="VFB89_15210"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HZM28696.1"
                     /translation="MAVHSILVAGHLCIDISPELSRVPELVPGQLYEIGSAQLTLGGC
                     VANTGLALAAAGVPTRVCGCVGDDPLGEIIRGLLAEQGVDLEEVMTTAEAATSYTIVL
                     QSPDHDRTFWHHSGANARFTGREVKLDGVDILHLGYPPLLPALLDDDASPLLDLLQEA
                     RSRGITTSLDLAVVDRRSKAADVNWRRVFTKIMPYVDIATPSIDDLTSALGITQVPDD
                     DLVEECARRLLDDGCAVAVVSAGSRGAYAISAGADRLTEAGTALSPVRSSWSSTAEWV
                     PAATPSRVVSTTGAGDAASAGLLYGLAQGAGLRQATRLAAHFAAASIEGKRPSAEDAG
                     RWAAELAAAGLD"
     gene            complement(1111..2262)
                     /locus_tag="VFB89_15215"
     CDS             complement(1111..2262)
                     /locus_tag="VFB89_15215"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZM28697.1"
                     /translation="MTKIVNIAIVGGGLMGREIAAALQRWPALIDHPVRPQLIAVCDI
                     NPAALDWFDQIPTVTSKVTDYADLLGDDAIDVLYIAVRHDLHQQLYVDTIQAGKSLLA
                     EKPFGIDGSAAAAIMAAAADNPQSFVRCASEFPFFPGAQLAVNYVRSGALGRIIEARC
                     AFLHASDLDINKPINWKRQAQYCGEAGVMNDLGLHVWHVPLRLGWTPDRIFGILQDIV
                     TERPVPNGGPERCDTWDNAVLHGWVNTDGYTFPLTTEMKRIAPGEKNTWEFEALGMDG
                     GVRFSTKNPKRVEVFSIANVPGSGREQVWSSLDAGSQSVWPSVTGPNFESGFSDAILQ
                     MLAAFLAEREGALSDRFGCATPEEAAVTHAIYRAAMQSHETGEVARVGG"
     gene            complement(2259..>3042)
                     /locus_tag="VFB89_15220"
     CDS             complement(2259..>3042)
                     /locus_tag="VFB89_15220"
                     /inference="COORDINATES: protein motif:HMM:NF013916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28698.1"
                     /translation="AVDHGFFGERSFLTGIEDMDAVVRTLVAANPDAVQLSLGHARLL
                     QAVPGKQKPALVLRSDVANVYGNPLDEHLFSHHIPNAIEEAVRLDAVAICANLMQLPG
                     RPEIREANISSIMALRAAAAMYAMPLMIEPLVMKDNAAAGGGYMVDGDTDKIMTLVRQ
                     ARELGADLIKADPTDNIEDYHKIIEVAGDVPVLVRGGGRVDDRTLLERTAAVLAQGAC
                     GIVYGRNVVQHENPSGITRALMAVLHDKTSVEDALRLVGAAR"
BASE COUNT          531 a          996 c          946 g          569 t
ORIGIN      
        1 tgtggttgaa gtcgatcacc gctccaccct ctcactcgaa cgggttggcc gccgatccct
       61 ttgagggctc tcagtccaag cctgctgcgg ccagctcggc agcccagcgg cccgcgtcct
      121 ccgccgaggg tcgcttgccc tcgatgctgg ccgcggcgaa gtgtgcagcg agccgagttg
      181 cctgccgaag cccggctcct tgtgcaaggc cgtagaggag tccggcgctg gcggcgtcgc
      241 ccgcacccgt cgtactgaca acccggctcg gcgtagccgc cggtacccac tccgccgtcg
      301 aactccatga agaccggacg ggcgagagtg ctgtcccggc ctcggttaga cgatcggctc
      361 cagcgctgat cgcgtacgct cctcgtgacc ctgccgacac gacggcgacg gcgcatccat
      421 cgtccaggag ccggcgagcg cattcctcca caagatcatc gtcgggcact tgagtgatgc
      481 cgagcgccga cgtcaggtcg tcgatgcttg gagtagcgat gtcgacgtac ggcatgatct
      541 tggtgaaaac tcgccgccag ttcacgtcgg cggctttgga ccgccgatca actacggcca
      601 gatcgagaga cgtggtgatc cctcggctgc gggcctcctg gagcagatca agtagcgggc
      661 tcgcgtcgtc gtccagtaag gcaggcagca gtggcggata gccaagatgg aggatgtcga
      721 ctccgtcgag cttcacttcc cggcctgtga accttgcgtt ggcgccggag tgatgccaga
      781 aggtccggtc atgatcaggc gattgcaaca cgatcgtgta cgaggtcgcc gcctctgcgg
      841 tggtcatgac ctcctccagg tcgactccct gctcggccaa caagccgcgg atgatctcac
      901 cgagcgggtc gtcgccgaca catccgcaga cacgagttgg tacgccagct gcagcgagcg
      961 caaggccagt gttcgctaca catccgccga gagtgagctg tgccgagcca atctcgtaga
     1021 gctggcctgg caccagctcg ggaacacggc tgagttcagg actgatgtcg atgcaaaggt
     1081 gcccagcgac aagtattgag tgcacagcca tcagccgccc acccgtgcga cctcgcccgt
     1141 ctcgtgcgat tgcatcgccg cgcggtagat cgcgtgcgtg acggcggcct cctccggtgt
     1201 ggcgcaaccg aaccggtcgc tcagagcccc ttctcgctcg gcgaggaacg cggccaacat
     1261 ctgcagaatg gcgtcggaga atccggactc gaaattcggg cccgtcacgc tcggccacac
     1321 cgattggcta ccggcgtcga gggaagacca gacctgttca cgaccactcc ccggcacatt
     1381 tgcaatggag aagacctcca ctcgcttcgg gttcttggtc gagaatcgca ctccgccgtc
     1441 catgcccagc gcctcgaatt cccaagtgtt cttctctccc ggggcgatcc gcttcatctc
     1501 cgtcgtcagc gggaacgtgt agccatccgt gttcacccac ccgtgtagga ctgcgttgtc
     1561 ccaggtgtca caccgttcgg ggccgccgtt cggtaccggc cgttcggtca cgatgtcctg
     1621 caggatccca aagattcggt ccggcgtcca gccgagtcgc agcggcacgt gccacacatg
     1681 cagcccaaga tcattcatga cgccggcctc gccgcagtac tgtgcctgtc gcttccagtt
     1741 gattggcttg ttgatgtcaa gatcacttgc gtgtaggaag gcgcagcggg cctcgatgat
     1801 ccggcccagg gcgccagagc gcacatagtt gaccgccagc tgcgcacctg ggaaaaacgg
     1861 aaactcgctt gcgcaccgca cgaatgactg cgggttgtcc gcggcggccg ccatgatcgc
     1921 tgctgcggcg gacccatcga tgccgaatgg cttctcggcg agcaggctct tgccggcctg
     1981 gatggtgtcg acatagagct gttggtgtag gtcgtggcgt acggcgatat aaagcacatc
     2041 aatcgcgtca tcgccgagca ggtcggcgta gtcggtcacc ttgctcgtca cggtgggtat
     2101 ctgatcgaac cagtccaggg cagccgggtt gatgtcgcag actgcgatca gctgcggacg
     2161 tactgggtgg tcgatcagcg cgggccaccg ttgcagcgcc gcggcgatct ctcgtcccat
     2221 gagaccgccg ccaacgatgg caatattcac gatcttggtc accgcgccgc tcccaccagg
     2281 cggagggcgt cctcgacgct ggtcttgtcg tggaggacgg ccatcagagc tcgagtgatg
     2341 ccagacggat tctcgtgttg cacgacgttt cggccgtaca cgatgccgca ggctccctgg
     2401 gccagcaccg ccgccgtccg ctccagcagg gtgcggtcat cgacacgtcc accgccacgt
     2461 accagcacgg gaacatcgcc ggcgacctcg atgatcttgt gatagtcctc gatattgtcg
     2521 gtcgggtcgg ccttgatcaa atcggctccc agctctcgag cctgacggac cagggtcatg
     2581 atcttgtcgg tgtcgccgtc gaccatgtat ccgcctccgg cggcggcatt gtccttcatc
     2641 accagcggtt cgatcatcag cggcatggcg tacatggccg ccgcggctcg gagagccatg
     2701 atcgaggaga tgttggcctc ccggatctcg ggtcgccccg gcaactgcat caggttggca
     2761 cagatcgcaa ccgcgtccag gcgtaccgcc tcctcgatcg cattcgggat gtggtggctg
     2821 aacaggtgct cgtccagtgg gttcccatac acgttcgcaa catcacttct caagacgaga
     2881 gccggcttct gctttccggg aacggcctgc agcaggcggg cgtggcccag actcagttgc
     2941 acggcatctg gattcgctgc caccaaggtg cgaaccacgg catccatgtc ctcgataccg
     3001 gtcaggaagg accgctcacc gaagaagccg tggtctacgg cc
//
LOCUS       DASRJQ010000672         6080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1532421, whole genome shotgun sequence.
ACCESSION   DASRJQ010000672 DASRJQ010000000
VERSION     DASRJQ010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6080
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1532421"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VFB89_15225"
     CDS             <1..245
                     /locus_tag="VFB89_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28699.1"
                     /translation="DDRRVAGICRRYEMRSVQLKGLRVVGRPTAAEKQGLVTATEYEK
                     LRNLIPQVLRVDRRCTEEDQRCRQQSKAHSGSRVLV"
     gene            complement(271..2121)
                     /gene="nagB"
                     /locus_tag="VFB89_15230"
     CDS             complement(271..2121)
                     /gene="nagB"
                     /locus_tag="VFB89_15230"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HZM28700.1"
                     /translation="MSAPSGIERLRTVVVEHYDDIGRLVAQRIADVIHASREAGSRPV
                     LGLATGSTPIGVYRELIRMHREEGLSFAQVVTFNLDEYYPMDPQSVHSYHRFMWENLF
                     SQLDIAPQNVHIPRGDLPRHEVEAECRRYEDAIRAAGGIDLQLLGIGRTGHIGFNEPG
                     SGVESRTRVVTLDLVTRKDAAADFFGEDNVPREAITMGVATILEAREIVILATGEHKA
                     DIVRRAVEGDVDHEIAATFLQQHPHTSCYLDRPAAASLTRFATPWVLGPVDWTPDLIV
                     RATCWLSRQTGKGILKLTRRDYAEHRLSSLVAKVGSPGVLNGQVFNALGAKIRGRSKL
                     PSDRRIICFSPHPDDDVISMGGILRKLIENGNRISVAYMTSGNIAVFDHDVRRHIDFL
                     RRLAWERRLAEDAVGELCRRVEEFLARKQPGDVDTPEVQDLKRMIRESEAVAGIRTLG
                     LDAAAARFLNLPFYRTGQVRKDPVGPADVAIVRNLLGEIRPDLVFVAGDLSDPHGTHR
                     MCKEAIDQALTEYVAAGDSRPEVWLYRGAWQEWPVPEATWLVPLSQEELRLKIQAIFK
                     HQSQKDSAPFPGPDEREFWQRVEARNTGTAAELDRLGLAEYFAMEAFVVE"
     gene            complement(2166..3101)
                     /locus_tag="VFB89_15235"
     CDS             complement(2166..3101)
                     /locus_tag="VFB89_15235"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing GNAT family
                     N-acetyltransferase"
                     /protein_id="HZM28701.1"
                     /translation="MPPSPSPVDQRAAALRRFNRFYTRHIGVLNEGLLESPFSLAEAR
                     VLYELAHRTRPTASDLSAALGLDPGYLSRLLRGLRRRSLVARERSEADGRRSHLALTR
                     KGRAAFAKLDTRSHDDMRALLTRLGEPEQRRLLEAARTMERLLDPAAVPAPSYILRFH
                     QPGDMGWVVHRHGALYTQEYGWDEQFEALVAGIVAEFLRKFDPRKERCWIAEREGDIL
                     GSVFLVRDSDALAKLRLLLVEPHARGLGIGGRLVAECVGTARRLGYRRLTLWTQRSLA
                     AARHLYQQAGFSLVRSEPHHSFGHDLVGETWDLEL"
     gene            3140..4540
                     /locus_tag="VFB89_15240"
     CDS             3140..4540
                     /locus_tag="VFB89_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZM28702.1"
                     /translation="MTATVNKEGGSGAAAGATTYARRLGLFSGTMAVIGGIIGAGIFR
                     TPAAVAERVPSSTATLGAWILGGAIALLGAFCFAELGQRRPKAGGGYVYLREAWGRLP
                     AFLYGWGLLLVIATGAIAAVAVTFASYVLALGGLSDRFTVPVAVAAIILLSAINYVGV
                     KPAALTQNVFTVLKLVALAALIACGLSAAPYHPPAPPRPSAAEAYSLFAGIGAALTPI
                     LFTYGGWQQTNFIAEEIVDPERTLPRAIILGVTGVVIVYLLANLSYLRVLGHAGLAAS
                     TAPAADLMRARLGPIGGTLIAAGIAASSFGFLNLVILVTPRVFQAMAADGLFFSRLAK
                     LHPIYRTPTAAILLQAAWAVVLTLTGTFAQLVDYVAFGDWIFFGLTAAAVFRYRLRDS
                     HMEAVGPSGSFRVPGHPWTTGLFVAAAAYVVISSVLASPRNALIGSGLLALGFPVYGY
                     WHGRSRSRMHGEHQRS"
     gene            4712..5344
                     /locus_tag="VFB89_15245"
     CDS             4712..5344
                     /locus_tag="VFB89_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZM28703.1"
                     /translation="MAEPTPIRVLIADDHPLFRDGLAALLQKDSSIVVVGAAGNGSEA
                     VELFQSLQPDVVLVDLRMPPEDGIAVVEDLRAVSPRARVIVLSAFDADDDVLRAIQAG
                     AAGYLLKMSSPSELAQAILTVHRGQTALPPGVATKLASHVSRPRLTGRQEEVLQLIAR
                     GMTNQEIGQELHIVEGTVKAHVKSILEKLGARDRTQALCIALERGLVRAN"
     gene            complement(5345..>6080)
                     /locus_tag="VFB89_15250"
     CDS             complement(5345..>6080)
                     /locus_tag="VFB89_15250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZM28704.1"
                     /translation="MMTRGRILLADDEETFLHATADLLRREGYECETVPDAPSALAKV
                     KTSPYDLLISDLEMPGNENLALIREVGDVAGGLPVIIITGFPSTTSAIAAIELPVSAY
                     LLKPVALPELLDRVASAVARFRSYQVMRRTEDRLRQWNADFERISSLQADGAAKPGKE
                     VDAFLALTLRNVMGSLSDLQQLGRALANSNQAEHACQILNCPRGTQLQEAVRETVRVL
                     EETKGAFKSKTLGDLRHKLELLLDHN"
BASE COUNT         1032 a         1947 c         1970 g         1131 t
ORIGIN      
        1 ctgatgaccg tcgcgttgct ggcatttgcc gaaggtatga aatgcgatcc gtgcaactga
       61 agggcttgag ggttgttggg cgccccactg ccgccgagaa acaggggctc gtcaccgcca
      121 ccgaatatga aaagctgcga aatctgatcc cgcaggtgct gcgcgtggac cggcgctgca
      181 cggaagagga ccagcgctgt cgtcaacaga gcaaggcgca cagcggctcc cgagtcttgg
      241 tttgaacgga gaccctgatt tacagtggcg ctactctacc acaaatgctt ccatcgcgaa
      301 atactcggcg agccccagcc gatcgagctc agcagcggtt ccggtattgc gggcctcgac
      361 ccgctgccag aactctcgtt cgtccggccc cggaaacggc gccgaatcct tctgcgactg
      421 atgcttgaag atcgcctgga tcttgagccg cagctcctcc tgcgacagcg gcaccagcca
      481 cgtggcttcg ggcaccggcc attcctgcca ggcgccgcga taaagccaga cctccggtcg
      541 gctgtctccg gcggcaacgt actccgtgag tgcctgatcg atcgcctcct tgcacatccg
      601 atgagtacca tgaggatctg agaggtcgcc tgcgacgaaa accagatccg gcctgatctc
      661 cccgagcagg ttccggacga tcgcgacgtc ggccgggccg accgggtctt ttcgaacctg
      721 gccagtccgg tagaacggga gattcaggaa gcgagcagcc gcggcatcga ggcccagcgt
      781 tcgaatgccg gcgactgcct ccgactcgcg aatcatccgc ttgaggtcct gtacctccgg
      841 cgtgtccacg tcacctggtt gcttgcgagc cagaaactct tcgacccgcc ggcacagctc
      901 gcctacggcg tcctcggcaa ggcgtcgctc ccaggccagc cgccgaagga agtcgatgtg
      961 gcgccgtacg tcatgatcga agacggcgat gttgccgctg gtcatgtaag cgacactgat
     1021 gcggttcccg ttctcgatca gcttgcgcag aatgccgccc atcgagatca cgtcatcgtc
     1081 gggatgcggc gagaagcaga tgatgcgacg atcggacggg agcttcgagc gacccctgat
     1141 cttggcaccg agtgcgttga acacctggcc gttgaggacg ccaggtgagc cgacctttgc
     1201 caccagagag gacaggcgat gctcggcgta gtcccggcgg gtcagcttga ggatgccctt
     1261 gccggtctga cgggacagcc agcaggtggc tctgacgatg aggtccggag tccagtctac
     1321 cggccccagc acccacgggg tggcgaagcg agtgagggac gccgccgccg gccggtcgag
     1381 atagcagctg gtgtgcggat gctgctgcag gaaggtcgcg gcgatctcgt gatccacgtc
     1441 gccttccaca gcgcgacgca cgatgtcggc cttatgctcg ccagtggcca ggatcacgat
     1501 ctcgcgggcc tcgagaattg tggccacgcc catcgtgatc gcctcgcgcg gcacgttgtc
     1561 ctcgccgaag aagtcggcag cggcgtcctt gcgggtgacc aggtcgagcg tcaccacgcg
     1621 ggtccggctc tcgaccccgg agcccggctc gttgaagccg atgtgaccgg tgcgcccaat
     1681 tccaaggagc tggagatcga tgccacccgc tgcgcggatc gcgtcctcat agcggcggca
     1741 ctcagcctcg acctcgtggc gggggaggtc accgcggggg atgtggacgt tctggggagc
     1801 aatgtcgagt tgggagaaca gattctccca catgaaccga tggtacgagt gcacgctctg
     1861 cggatccatc gggtagtact cgtcgagatt gaacgtcacc acctgcgcaa aactcagccc
     1921 ctcctctcga tgcatccgga tgagctcgcg atataccccg atcggggtgg agccggtcgc
     1981 caggcccagc accgggcggc tgccggcttc cctgctggcg tgaataacgt cggcgatccg
     2041 ttgggcaacc agccgcccaa tgtcgtcgta gtgctcgacc accacagtgc gaagccgttc
     2101 gatgccggag ggtgcgctca tgccataaag tctgagcgag ccggccggtt tcacaacccc
     2161 gacggctaca actcaaggtc ccaggtttcg cccacgagat cgtgaccgaa gctatgatgc
     2221 ggctcgctcc ggaccagcga gaaccccgcc tgttgataga gatgccgggc cgctgcgagc
     2281 gatcgctggg tccagagggt gagcctccga tagcccagac ggcgggccgt tcctacgcac
     2341 tccgccacca accggccgcc gatgccgagt cctcgtgcgt ggggctcgac cagcaggagg
     2401 cgcagcttgg ccagggcgtc ggagtcgcga accaagaata ccgagccgag aatgtcgccc
     2461 tcgcgctctg cgatccagca ccgttccttc ctgggatcga acttccggag gaattcggcc
     2521 acgataccgg cgaccaacgc ctcgaactgc tcgtcccagc cgtattcctg ggtgtatagc
     2581 gcgccgtgcc ggtgcacgac ccagcccatg tcgcccggct ggtggaagcg cagaatgtac
     2641 gagggcgccg gcactgccgc ggggtcgagc agccgttcca tggtgcgggc ggcctcgagc
     2701 aggcgccgtt gctccggctc tcctagccgg gtcagcaggg cgcgcatatc gtcgtgcgat
     2761 ctcgtatcca gtttggcaaa ggctgctcgt cctttgcggg tcagggccag gtggctccgc
     2821 cggccgtcgg cctcggaccg ctctcgcgcc accaacgagc gccgccgcag tccgcgcagc
     2881 agtcggctca ggtatccggg gtcgagcccg agagcggcgc tgaggtcgct cgcggtcggg
     2941 cgggtccgat gggccagctc atagagcacg cgcgcttctg cgagagagaa cgggctctcg
     3001 agcaggcctt cgttcagcac accgatgtgg cgggtgtaga accggttgaa acggcgcaac
     3061 gcggcggcgc gttgatcgac gggagacgga gagggaggca tacccgggcc cttgacgatt
     3121 ccttgccaat gtcaactaaa tgactgctac tgtcaataaa gaaggtggct ccggcgcggc
     3181 tgcaggggcg actacctacg cccgccggct gggactcttc tcgggaacca tggccgtgat
     3241 cggcgggatc atcggcgccg gcatcttccg aacccctgct gcggtggctg agcgggttcc
     3301 ctcgagtacc gccacgctgg gtgcatggat cctcggcggg gccattgccc tgctcggcgc
     3361 gttctgcttt gccgagctgg gccagcgccg ccccaaggca ggcggcggct atgtctactt
     3421 gcgcgaggcc tggggacgct taccggcctt tctctacgga tgggggctgt tgctggtgat
     3481 cgccaccgga gccatcgcgg ccgttgccgt gacgttcgcg agctacgtgc tcgccctcgg
     3541 cggactgtct gaccgcttca ccgttcccgt agcggtcgcc gcgatcatcc tgctatccgc
     3601 catcaactac gtgggtgtca aacccgcggc cctaacccag aacgtcttca ctgtactcaa
     3661 gcttgtcgca ttggcggcac tcatagcatg cggactgagt gcggcgccat atcatccgcc
     3721 agccccaccc cggccttccg ctgcagaggc gtactccctc ttcgcgggaa tcggagcggc
     3781 gctcacaccc attctcttta cctacggggg ttggcagcag accaacttca ttgcggagga
     3841 gatcgtagac ccggaacgga cgctaccgcg agcgatcata ctgggtgtca cgggtgtggt
     3901 gatcgtctat ctgctcgcca acctgagcta tctgcgggtc ctgggccacg ccggtctcgc
     3961 tgcgagcacc gccccggccg cggatctgat gcgtgcaagg ctcggaccga tcggagggac
     4021 cttgattgcc gccggcatcg cggcctcgag cttcgggttt ctcaatctcg tgatcctcgt
     4081 gacgccccgc gtcttccagg ctatggccgc tgacggtctg ttcttttccc gcctggcaaa
     4141 gcttcatccg atctaccgga cgccgaccgc agccatcctg cttcaggccg cgtgggccgt
     4201 ggtgctgacg ctgaccggca cattcgcgca gttggtcgat tacgtcgcgt tcggtgactg
     4261 gatcttcttt ggactcacgg ccgcagcggt gtttcgttat cggctgcggg atagccacat
     4321 ggaagcggtc ggaccatccg gttcgttccg ggtcccgggg catccctgga ccacaggtct
     4381 ctttgttgcg gcagcggcct atgtcgtgat cagctcggtg ctggcaagtc ctcgcaacgc
     4441 gctgatcggg tcaggactcc tcgctctcgg ttttccggta tatggttatt ggcatgggcg
     4501 cagccgcagt cggatgcacg gtgaacatca gcgctcgtga ggggtgtcac atcctgtcgg
     4561 gtcgaagatc cgtaacgttg attaacgatc cttgtcggat gacatgatga gcatgtacgc
     4621 tgaaggcgca acgaagctcc caacggggga ggtcggtgcg cctttttctc ggcctcccag
     4681 tccctctccc ggcagccaag gagactctcg agtggctgag ccaacgccca ttcgcgtcct
     4741 cattgccgac gatcatccgc tgttccgaga cggcctcgcc gcgttgctgc aaaaggactc
     4801 ttcgatagtc gtggtcggag ctgccggtaa cggatccgaa gcggtcgagc tgtttcagtc
     4861 gctccagccg gacgtcgtac tggtggactt gcggatgccg cccgaggatg ggatcgccgt
     4921 tgtcgaggat ttgcgggcag tgtcgccgcg ggcacgggtc atcgtgctgt cggcattcga
     4981 cgccgacgac gatgtcttac gggcgattca ggccggggcc gccgggtacc tgctcaagat
     5041 gtcctcgccc agtgagctgg cccaggcgat cctcaccgtg catcgaggcc agaccgcctt
     5101 gcccccgggc gttgcgacca agctcgcgag tcatgtgagt cggccacgcc tcaccggtcg
     5161 gcaggaggag gtgctccagc taattgcgcg cggcatgacc aatcaggaga tcggccagga
     5221 gctgcacatt gtggagggaa cggtcaaagc ccacgtcaag agcattctcg agaagctcgg
     5281 cgccagggat cgaacccagg cgctctgcat tgcgctcgag cgggggctgg tgcgggccaa
     5341 ctgatcagtt gtgatccagc agcagctcca gcttgtggcg tagatcgccc agggtcttgg
     5401 atttgaacgc gcccttggtt tcctccagca ctcgcaccgt ttccctcact gcctcttgca
     5461 gctgcgttcc ccggggacaa ttgagaatct ggcaggcgtg ctcggcctga ttggagttgg
     5521 ccagggcgcg gcccagctgc tgaaggtcgc tcaacgagcc catcacgttc cgcagcgtca
     5581 aagccaggaa tgcatccact tccttgcccg gcttggctgc accgtcggcc tgcagtgagg
     5641 agatccgctc gaaatccgcg ttccattgcc ggagccggtc ctcggtacgc cgcatgacct
     5701 ggtaactgcg gaagcgcgcg actgccgagg cgacgcgatc gagcagttcg ggcagggcga
     5761 cgggtttgag gagataggcc gagaccggca gctcgatggc ggcgattgcc gaggtggtcg
     5821 agggaaagcc ggtgatgatg atgaccggca atccgccggc cacatcgccc acctcgcgaa
     5881 tcagcgcgag gttttcgttt cccggcatct ccagatcgct gatcaggaga tcgtagggcg
     5941 aggtcttgac cttggccagg gcactcggcg cgtcgggcac cgtctcgcac tcgtaccctt
     6001 cacggcgaag cagatccgcc gtcgcatgga gaaatgtctc ctcgtcgtct gcgaggagta
     6061 tccggccgcg cgtcatcatg
//
LOCUS       DASRJQ010000673         2123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1380520, whole genome shotgun sequence.
ACCESSION   DASRJQ010000673 DASRJQ010000000
VERSION     DASRJQ010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1380520"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VFB89_15255"
     CDS             complement(<1..839)
                     /locus_tag="VFB89_15255"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZM28705.1"
                     /translation="MDKPTPDAPTQAAPERPTEAPKPRPAVAGRIPGIIVGLVVAVVA
                     GLSIWYLVRPQSLLVQGEVDATRFDIAARVDGRVADIPVVRGQNVPAGAVLVRIDNPE
                     TLAKNEQALAAKVVAEAQLANINAGTRAEVIAARKAALERAQASVVLAKKTYDRVSQL
                     AEHGNAPQARLDQATDSLHESERGVDQAKSAYEQAVNGYTREEREIAVANVQKAIADI
                     TAVQAIIDQMVVYAPVASQVYKRNVEPGEYVSPGVPLITLIDLNDMWVHFDLREDLVR
                     ALKV"
     gene            1244..1393
                     /locus_tag="VFB89_15260"
     CDS             1244..1393
                     /locus_tag="VFB89_15260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28706.1"
                     /translation="MNKTNDVVPTRELTEAEVDAVSGGWFAFHATTEAIDALAKALAT
                     TAQKQ"
     gene            1538..1702
                     /locus_tag="VFB89_15265"
     CDS             1538..1702
                     /locus_tag="VFB89_15265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28707.1"
                     /translation="MAATSNQVREPTNQADELTADELEHVSGGKGGKGDPKAQEYLVV
                     TLKEVMISSV"
     gene            1921..2103
                     /locus_tag="VFB89_15270"
     CDS             1921..2103
                     /locus_tag="VFB89_15270"
                     /inference="COORDINATES: protein motif:HMM:NF017454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HZM28708.1"
                     /translation="MTTAIKAPAARGRENQPRELTEVELSHVTGGGSSGADAGKVTFN
                     PFSITKTIDKASPVLF"
BASE COUNT          384 a          691 c          691 g          357 t
ORIGIN      
        1 accttgagcg ccctgaccag gtcctcgcgc agatcgaagt ggacccacat gtcgttgaga
       61 tcgatcagcg tgatcagcgg cacgccgggc gacacgtatt cgccgggctc cacgttgcgt
      121 ttgtagacct gcgacgccac cggcgcgtag accaccatct gatcgatgat cgcttgcacg
      181 gccgtgatgt cggcgatcgc cttctgcacg ttcgccacgg cgatctcgcg ctcttcgcgg
      241 gtatagccgt taaccgcctg ctcgtaggcc gatttggcct gatcgacgcc acgttcgctc
      301 tcgtgcaggg aatcggtggc ctggtcgagg cgagcctgcg gcgcattacc atgctcggcc
      361 agctggctga cgcgatcata ggttttcttc gccagcacga cgctcgcctg cgcccgctcc
      421 agtgcggcct tgcgcgccgc gatcacctcc gcgcgtgtgc cggcattgat gttggcgagc
      481 tgcgcctcgg ccacgacctt ggcggccagc gcctgctcgt tcttcgccag cgtctcggga
      541 ttgtcgatgc gcaccagcac cgcgccggcg gggacgttct ggccgcgcac gactggaata
      601 tccgcaacgc ggccatcgac ccgcgcggca atgtcgaagc gcgttgcatc cacctcgccc
      661 tgcaccagca gggactgcgg ccgcaccaga taccagatcg acaggccggc gacgacggcg
      721 acgaccaggc cgacaatgat gcccggaatg cggccggcga cggcgggccg cggcttcggt
      781 gcttccgtcg gccgctcggg cgcggcttgc gtgggagcgt caggcgtcgg cttgtccatt
      841 ccagctcgtc cagccccggt tggcagtggt gtcgatgagc gccggttaca tcggatcagc
      901 tccgggcgac acggcacgag cggagtgtct accatatccg cgccggctgc agccacacac
      961 agccgtgaga gcgacggacg gaacggcgaa aatcgcttcg gatcgaaccg cgcagtttcg
     1021 ctccatgctg tcgccattat cggtgcggtg atcgcgctcg ttgcgctgat cgtgctcggc
     1081 ttggccttca gctcgcgcgc cagggcagat tgtgatggcc atcacaaaaa gtctgatttg
     1141 gccgcagtca gtgagcggct tcactgttgc gcccaggggc gctgagcgag tgtcgccgcg
     1201 ccggcgatcg gatgatccgt tcgctggcgc aatggagacc acgatgaaca agaccaatga
     1261 cgtcgtgccg acacgcgaac tgaccgaagc cgaggttgac gccgtcagcg gcggctggtt
     1321 cgcgtttcac gccaccaccg aggcgatcga tgcactcgcc aaggcgctgg cgaccacggc
     1381 ccagaagcag tgatggagcg ggtgatgacc actggaattg acgagctgcg cgacgacgag
     1441 ctcgatggag tgaccggcgg tctggttgtg atcgcgatca tcgccatcct gatcggtccg
     1501 ttgatccctg atcacatcta acgacatgga gctgacaatg gctgccacaa gcaatcaggt
     1561 gcgcgaaccg acgaaccaag cggacgaact gaccgccgac gagctcgagc acgtctcggg
     1621 tggaaagggc ggaaagggcg acccgaaagc ccaggagtac ctcgtcgtta cgctcaaaga
     1681 ggtgatgatc tcgtcggtgt agcggggtct cgacccaacg agcgcactgc atgcttggcc
     1741 gcccggggca accgggcggc ccctttgcac aggagcccgg tgccgttgtc gaaatgcacc
     1801 tagtccgcac tcatggtgaa gaagctcaca caccaacccc tgccccgccg cgactcatcg
     1861 atgcgggcag cgcgccgggc agcactggcg cggatatcac cagcaaatgg agcagctgca
     1921 atgaccacag caatcaaggc ccctgcggcg cgtggacggg aaaatcagcc acgcgagctg
     1981 accgaggtcg agctgtccca cgttaccggc ggcggctcga gcggcgcgga cgcgggcaaa
     2041 gtcacgttca acccgttctc catcacgaag acgatcgaca aggcctctcc cgtgctcttc
     2101 tagagggcgg gaccgggggg atg
//
LOCUS       DASRJQ010000674         2896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_544137, whole genome shotgun sequence.
ACCESSION   DASRJQ010000674 DASRJQ010000000
VERSION     DASRJQ010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544137"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1390
                     /locus_tag="VFB89_15275"
     CDS             <1..1390
                     /locus_tag="VFB89_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28709.1"
                     /translation="SWQDTLKSAQNNLTQDLGNLETKAARDLNDQQKQIVDDVHGLIE
                     QVQGVVKDGVEFTQESLNCQTDIFASHARTAVMNMLNAFLNKYKYKGDANRPMIPYVP
                     IVCSTNPTGINVGKWSADTFLVLSGTDLSIFDTKAPEIVLVGPSGNEWQAPFGGNRET
                     NYRFAVNVQPLIAQGLMKDAVQLQVRWSGQRVNRNEIPIVPCGGIGRPCCRGTCDQGA
                     CVNDVCQSCGAPSQHCCPGGACSTGTTCVSTVCSACGGIDQPCCHGAQCSVGTCLNQQ
                     CTACGDLGQSCCYGRQCNGGAVCESGRCAAKREACMWTADFSEEGVAEQHCPSGSAMS
                     GARCTGSYCDNVALWCCPYLAGPDASASKSWSGSFSEENGGRLYTPAQASQFVAGLRC
                     RGDNCDNLQMEYIRTPHLAFGGACYESPLFSEEGHNYFTCLAGRWVGGMACRGDYCDS
                     MSVYCCQGTPVH"
     gene            2072..2704
                     /locus_tag="VFB89_15280"
     CDS             2072..2704
                     /locus_tag="VFB89_15280"
                     /inference="COORDINATES: protein motif:HMM:NF039733.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6338 family protein"
                     /protein_id="HZM28710.1"
                     /translation="MLRFLLPGFLAGWVYFGLTAHPKPSEFERVVQALIFTVIIQGVV
                     AGIRSSSLYVGSHWRVVGRWTDDVSLVWATVVALGVGLLVVRWANNDWIHRQLRKFRF
                     TQETSFPSEWYGVLRQRPTYVVLHLSGERRLYGWPEEWPSDPQKGHFSMAEGEWLDDE
                     NKRTPLAGVSNILVPATEVSFVELMEPVHRPRETENHEPQSAPTSPEQAN"
BASE COUNT          569 a          832 c          971 g          524 t
ORIGIN      
        1 gtcctggcag gacacgctga aatcggccca gaacaatctc acgcaggacc ttggaaacct
       61 cgagacgaag gccgcgaggg acctcaatga tcagcagaag cagatcgtcg acgacgtgca
      121 cgggctgatc gagcaggtcc agggggtggt gaaggacggc gtggagttca cgcaagagtc
      181 cctcaactgc cagactgaca tcttcgcctc tcacgcgcgc accgcagtga tgaacatgct
      241 caacgcgttc ctgaacaagt acaagtacaa gggcgatgcg aaccgtccga tgattcccta
      301 cgttccgatt gtgtgttcca caaatccgac cggtatcaac gtcggcaagt ggagcgcgga
      361 cacgttcctc gtcctgtcgg ggaccgactt gagcatcttc gacacgaaag cgccggagat
      421 cgtgctcgta ggaccttcgg ggaacgagtg gcaggcgcct ttcggcggga accgggagac
      481 gaactatcgg ttcgcggtca acgtgcagcc gctcatcgcg caagggctca tgaaggacgc
      541 cgtccagctc caggttcgct ggagcggcca gcgagtcaat cgaaacgaga tccccatcgt
      601 cccctgcggt ggaatcggcc ggccctgctg ccgcggaacg tgcgaccaag gtgcctgtgt
      661 caacgatgtg tgccagtcct gcggagcgcc gtcgcaacat tgctgccccg gtggcgcgtg
      721 cagcacgggt acgacctgtg tgagcactgt ctgcagcgcg tgcgggggca tcgaccagcc
      781 ctgctgtcac ggcgcccagt gcagcgtcgg aacgtgcctg aaccagcagt gcacggcatg
      841 cggcgacctg ggtcagtcgt gctgctatgg tcggcagtgc aacgggggcg cggtgtgcga
      901 atcgggccgg tgcgcggcga agcgcgaggc ctgcatgtgg accgcagact tctccgagga
      961 aggggttgcc gagcagcatt gcccgtccgg atccgccatg agcggcgcga ggtgtacggg
     1021 cagttactgc gacaacgtcg ccctctggtg ctgtccctac ctcgccggcc cggacgccag
     1081 cgcgtcgaaa tcgtggagcg gcagcttctc tgaagaaaac ggcgggcggc tctacacacc
     1141 ggcccaggcc tcgcagttcg tcgccggcct ccgttgccgc ggagacaact gcgacaacct
     1201 gcagatggag tacatccgta cgccgcacct ggcgttcggc ggcgcgtgct acgagagccc
     1261 gctgttctcc gaagagggtc acaactactt cacgtgcctg gccgggaggt gggtgggcgg
     1321 gatggcctgc cgcggcgatt attgcgactc gatgtcggtc tactgctgcc aggggacgcc
     1381 ggtacactga atcccgcggt cagacgactg gatgagccgg acgagctgtg tttcccgcgg
     1441 ggaaccggac ggacggagag accgagacgg cgactccatt tggtgaggct ggcgccctgg
     1501 tgggtggatg ggacgcgcag cttcccgaag cacgacttgc ttttggtgga acgtaagcgg
     1561 tggactcgtc agaccgggaa gcaagagccc ggcgccgcga tcgcgacgat cgaggccacg
     1621 ctggggcctt gccgcttgct ttctccgagg acgatccggc cgcaggagaa cctcgcgacc
     1681 gcgggcgagc acggtcgggc tcgtagccct ttatagcggg gggtggccac tggtacgatg
     1741 cgaagaccga gacggcgacc ccttcatcgt taggctggtg ccctggttgg tgggaccaac
     1801 cgcaggggtt gaatagaggg gagcggctat ggcctccatt ccgcggtacg cgcaggatgt
     1861 cccgcgtcac aagctcgtga aggtgctatg ccgcggtgac tgtgggtcga ctcgatttgc
     1921 agaagtgtcg aaaactccct gggagaagag cgggacgagc gttgacccag agctcttcgc
     1981 tacatgcctt cgctgcggat acaaagctcg ggataactac aactggacga gagcctgagt
     2041 aatggattgg gcctctggcg aaatagtcag cgtgctgcga ttcctcttgc cggggtttct
     2101 tgctgggtgg gtttacttcg gcctgaccgc acacccgaag ccgtccgagt tcgagcgggt
     2161 cgtacaggcg ctaatcttca ccgtcatcat tcaaggcgtg gtggcgggta tccgctcgtc
     2221 gtccctgtat gtgggttcac actggcgagt tgttggtcga tggaccgatg acgtgtcctt
     2281 ggtctgggcc acggtggtgg cacttggcgt cggcctacta gttgttcgtt gggccaacaa
     2341 cgactggata cacaggcagc tccgaaagtt cagattcacg caagagacat ccttcccatc
     2401 cgagtggtac ggagtgttgc ggcagcgtcc cacctacgtc gtgttgcatc tctcgggtga
     2461 gcgacgcctc tacggatggc cggaagagtg gccgagtgat ccacagaaag gccacttctc
     2521 gatggccgaa ggagaatggc tcgatgatga aaacaagagg actcctttgg ccggagtatc
     2581 gaatatcttg gttccggcaa cagaagtatc tttcgttgaa ttgatggagc cagttcatcg
     2641 cccacgggag accgaaaatc atgagccgca aagcgcccca accagccccg agcaggcgaa
     2701 ctaatttaac agtggcgcgg gagggaagaa ccgtgactgg agtgcggccg actatcgtgg
     2761 taaaaccacc gcctccacct ccgccgccga ggaagaagtg agaggtcgaa ctggggcgct
     2821 acgagaaggc cgggacgagg actcttcaag gatgaggctg gcgcactggg tggatggatc
     2881 cgaatacact actggt
//
LOCUS       DASRJQ010000675         2820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_122702, whole genome shotgun sequence.
ACCESSION   DASRJQ010000675 DASRJQ010000000
VERSION     DASRJQ010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122702"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VFB89_15285"
     CDS             <1..183
                     /locus_tag="VFB89_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HZM28711.1"
                     /translation="LEVPVSVPDVPANLLDPRRTWSDPAAYDTQARKLAAMFRENFEQ
                     FRAQVPASVAAAGPRI"
     gene            425..1723
                     /locus_tag="VFB89_15290"
     CDS             425..1723
                     /locus_tag="VFB89_15290"
                     /inference="COORDINATES: protein motif:HMM:NF039904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZM28712.1"
                     /translation="MALSPEFEVIRDPLWDNIRLDRAALAALDTPTVQRLRYVRQLGH
                     AFLVYPGATHTRFEHALGAYHLTRKALASLEERGELACVPEQDRMAVRMAALLHDIGH
                     YPFSHALEEAGFPSHEGLGVAKLVRGELGLRLRDMGDGFPDLVGALITQRSRSPLQGL
                     ISGSLDLDKIDYLSRDAFMCGVPYGKVDVDRLLASLTLVENDGRLEVGVHEKGVSALE
                     SLLFAKYQMYRNVYWHHAVRSATCMFKRAVRAAVARKAIDIAAIAEATDDALMELLLR
                     PANDRPDNEWTELSGAIRARRLYKRALDLPASDVPSGVQPWLDDADLTARVEDALAAE
                     LELPRGGLLLDFPSRSSMLGVDLPLRLRTGAIDRLTDEGRAGQLGLPRVADELYRSAQ
                     RFRVFVANPVTRSLNGILEVATLPPAEIETRLRAGKPLLH"
     gene            complement(1641..>2820)
                     /locus_tag="VFB89_15295"
     CDS             complement(1641..>2820)
                     /locus_tag="VFB89_15295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28713.1"
                     /translation="TLASRDARARETVVYAVFVDSIRALTDRYYRATAQKRVNPDTWK
                     ASLDSAARRLWPRLLWTGSVPKFTNERFEAELDRRFGALINLGETAGYQDLHMGHRVL
                     DDRRTVEQYGHTGRVRFVALDAIVSNGFQSWAWDGNAQHGGWATRDLIVQVRPAYADD
                     PLQAWRQTTDPETIRRLAEELAADSIADLAKAQRTPVAYFPSIEERLSRDGRRVLLDS
                     LRSTGLSGNELESAFKREYGRGLVESSIFAHEGRHAIDNNLPTKLTPAELEYRAKLSE
                     VAFAPRPRLALGGIISANAGDGTPHGQANQRVLEGLARWMDTHATEIRGLDRSAPYLP
                     QFPLLTDSQIKAAFAEQDPFAAHGKAARDSGFSAVEASQPGGGSRSRREAASPPPESH
                     "
BASE COUNT          469 a          946 c          918 g          487 t
ORIGIN      
        1 ctcgaagtac cggtttccgt tcccgacgtc cccgccaatc tgctggatcc gcgtcggacc
       61 tggagcgacc cagccgccta cgacacacag gcgcgcaagc tggcggcgat gtttcgggag
      121 aacttcgagc agttccgggc gcaggtgccg gcgtccgtcg ccgccgccgg gcctcggatc
      181 tagaccgctg ggcccgggcg ctcgcgagcg agcggaaccc ctccggggcc gcgccccctt
      241 cgaccattcg ggcgtctgca cgaacaagcg ctgtcggctc ggcggagggc cggagcgaac
      301 gggtgggggt ctgcagggcg cgttggtgag cggcggcgcg gttagatgca tgggtgactg
      361 agcgaaccac agcgcccgta tgatccctac ccgttcgccc cggcccactc caaagacaag
      421 accgatggcc ctttcgcccg aattcgaagt cattcgcgac cctctctggg acaacatccg
      481 gctcgatcgc gcggcgctcg cagcgctcga cacacccacc gttcagcggc tccgctatgt
      541 acgacagctc ggccacgcct tcctggtcta tcccggcgcg actcacaccc gcttcgagca
      601 tgctctgggt gcatatcacc tgactcgcaa ggcgctggcg tcgctcgaag agcggggcga
      661 gctggcatgt gtccccgagc aggaccggat ggcggtgcgg atggcggcgc tgctgcatga
      721 catcggccac tatccgttct cgcacgcgct cgaggaagcc gggttcccga gccacgaagg
      781 cctgggcgtc gccaagctcg tcagggggga gctgggcttg cggctgcgcg acatgggcga
      841 cggctttccc gacctggtgg gtgcattgat cacccagcga agccgaagcc cgctccaggg
      901 cttgatctca ggctcgctcg atttggacaa gatcgactac ttgagtcgcg acgccttcat
      961 gtgcggcgtc ccatatggca aggtcgacgt cgaccgcctg ctggcgtcac tcacgttggt
     1021 ggagaacgac ggacgactgg aggtcggcgt ccatgaaaaa ggtgtgagcg cactcgaatc
     1081 cctgctgttc gccaagtacc agatgtatcg caacgtgtac tggcaccacg cggtccgctc
     1141 ggcgacctgc atgttcaagc gggcggtgcg tgccgctgtc gcgcggaagg caatcgacat
     1201 cgcggcgatt gccgaagcga ccgacgacgc gctgatggag ctgctgcttc ggcccgcaaa
     1261 cgatcgaccc gacaacgagt ggacggagct ttccggcgca atccgggcac gccgcctcta
     1321 taagcgggca ctcgacctac cggccagcga cgttccttcc ggggtacaac cctggctgga
     1381 cgacgcagac ctgaccgcgc gcgtggagga cgcgctggcc gcagagttgg agctgccgcg
     1441 tggcgggctc ctgctcgact ttccttcgcg ttcgtcgatg ctgggcgtgg acctgccgct
     1501 ccgtcttcgc accggagcga tcgatcgcct gacggacgaa ggtcgcgccg gtcagctcgg
     1561 gttgccccgc gttgcggacg aattgtaccg gagtgcgcag cggtttcggg tcttcgtcgc
     1621 caatccggtg acgcgaagcc tcaatgggat tctggaggtg gcgacgctgc ctcccgccga
     1681 gatcgagacc cgcctccggg ctgggaagcc tctactgcac tgaatccgga gtcccgcgcg
     1741 gccttcccat gcgcggcaaa tggatcctgc tcggcaaatg cggccttgat ctgagagtca
     1801 gtcaggagcg gaaactgcgg cagatacggc gccgaccgat cgagcccgcg aatctccgtc
     1861 gcatgcgtgt ccatccacct tgccaatccc tcgagcaccc gctgattcgc ctgaccgtgc
     1921 ggcgtgccat ctcccgcgtt ggcactgatg atgccgccca gtgcgagacg aggccgcggt
     1981 gcgaaggcaa cctcggacag cttggcccga tactccagct ccgccggtgt aagcttggtg
     2041 ggcaggttgt tatcgatcgc gtgccgccct tcgtgcgcaa agatcgagct ttccaccaat
     2101 ccacggccat actcccgctt gaaggctgac tcgagctcgt tgcctgagag gccggtgctg
     2161 cggagcgaat caagcaagac ccggcgtccg tcacgcgaga gtcgctcctc aatgctcggg
     2221 aaatacgcga ccggggtgcg ttgcgccttc gcgaggtccg cgatgctgtc ggccgccagc
     2281 tcctcggcca ggcgacggat ggtctcggga tccgtggtct gtcgccacgc ctgcagcggg
     2341 tcgtcggcgt acgcaggacg tacctggacg atcagatccc tggtcgccca gccgccgtgc
     2401 tgggcgttcc catcccacgc ccagctctga aacccattcg acacgatggc atcgagtgca
     2461 acgaaacgga cgcggccggt gtgaccgtac tgctccacgg tccgccgatc gtcgagcacc
     2521 cgatgtccca tgtgcaagtc ctgatacccc gcggtctcgc ccaggttgat tagagccccg
     2581 aatcgccggt cgagctccgc ctcgaatcgc tcgttggtga acttcggaac ggagccggtc
     2641 cagagcaggc gaggccacag tcgccgtgcc gcagagtcga gcgacgcctt ccaggtgtcc
     2701 gggttcaccc tcttctgcgc ggttgcacga tagtaacgat ccgtgagagc gcggatcgaa
     2761 tccacgaaga cggcatacac caccgtctca cgtgcccggg catcacggga cgcgagggtg
//
LOCUS       DASRJQ010000676         3011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_4113520, whole genome shotgun sequence.
ACCESSION   DASRJQ010000676 DASRJQ010000000
VERSION     DASRJQ010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4113520"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..>3011)
                     /gene="secA"
                     /locus_tag="VFB89_15300"
     CDS             complement(135..>3011)
                     /gene="secA"
                     /locus_tag="VFB89_15300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HZM28714.1"
                     /translation="PDDREAAVRERMEEILPEAFAVVKDACRRHVGKSWSIVGIDVRW
                     DMIPFDVQLIGGAVLHEGKIAEMATGEGKTLVATLPLYLNALAGRGAHLVTVNNYLAR
                     RDSQWMGHVYKYLGLTVGCIDDTEPSSPARRDAYNADITYGTNNEFGFDYLRDNMVFS
                     LDQRVQRHHAYAIVDEVDSILIDEARTPLIISGPVGNEGDEKYTIFNRPVADLVRKQT
                     AVVNQLMAEAEPILPDEKKKEEAGYKLFQAQLGMPKNKKLLKLLNETGVKQLVQRAEL
                     DYIADRKLPTRQQRMRGLEDVLYYVLDEKGHSVHLTDRGAAALSPDDPEMFLVPDISE
                     EVGKIEKDPNLGIPEKRAERQRVEAEYAVKSEKLHIIHKLLQAHSLYEKDVDYLVQDN
                     QVLIVDEFTGRVLAGRRWSDGLHQAVEAKEAVQIKGETQTLATITIQNYFRMFDKLAG
                     MTGTAETEESEFYSIYGLEVAVIPTNKPIRRTDHTDRIYKTRREKFNAVLDEIERLHG
                     LGLPILIGTTSVDVSETLSKMLKRRGLTHEVLNAKYHEREAEIVAGAGQKAAITIATN
                     MAGRGTDIKLGKDLDLTTEEAGLQILGTERHESRRIDRQLRGRAGRQGDPGASLFFLS
                     LEDDLMRLFGSDRIARYMDRTGAEEGEVITGKLVMVAIESAQKRVELQNFQQRKRLLE
                     YDDVMNQQREVAYSLRLFALERGEELKAEARQMISRALEHAVRKFLEEAERPEDWDRK
                     GLVDALTLQFLVTPEALTDRALTPDLSAMVKAAVAEGEAAFHRKVEYLHEFGKGIGIA
                     DVDQQVLSQVTLAVIDEKWKDHLYDLDLLRNAIQYRAYGQKDPLVEYKKEAYEMFEDL
                     MRDIHSTFTERYLKIQVSAEPPRQAPPPPPPPIPTPRSGTDELFAGSTRTSTGPMGAP
                     PPPRVDTSMGRLGPSASGLPTVGRNDPCPCGSGRKYKKCHGAAV"
BASE COUNT          439 a          993 c          972 g          607 t
ORIGIN      
        1 gacgttcggc agtcgagagg gtggaatcgg ggagagggga aacaacagtc ggggtggaca
       61 tgcccgcaga gtaggcccgg ccggtgtcct ttcgagggcc tagatcacgc gggctggtcc
      121 gaatgccgaa cggttcacac cgccgcccca tggcacttct tgtacttccg ccccgacccg
      181 caggggcatg gatcgttccg gcccacggtc ggaagcccgc tggcggatgg ccccaaccga
      241 cccatcgacg tgtccacccg gggcggcggt ggggcgccca tgggtccggt gctcgtccgg
      301 gtcgaaccgg caaacagttc gtcagtcccg cttcgcgggg tcggaatcgg cggcggcggc
      361 ggcggcggcg cctgccgggg cggttccgcg ctgacctgga tcttgaggta ccgctccgtg
      421 aaggtggagt ggatgtcccg catcaggtcc tcgaacatct cgtaggcttc cttcttgtac
      481 tccaccagcg ggtccttctg gccgtaggcg cggtactgaa tggcgtttcg cagcaggtcg
      541 agatcgtaga ggtggtcctt ccatttctcg tcgatgacgg cgagcgtcac ctgcgacagc
      601 acctgctggt ccacgtccgc gatgccgatt cccttcccga actcgtggag gtactcgacc
      661 ttgcgatgaa acgcggcttc gccttcggcg accgcggcct tgaccatcgc ggacagatcc
      721 ggcgtgagcg ctctatcggt gagggcttca ggcgtcacca ggaactgcag ggtgagcgcg
      781 tcgaccagcc ccttgcgatc ccaatcttcc ggacgctcgg cctcctccag gaacttgcgc
      841 accgcgtgct ccagcgcgcg gctgatcatc tggcgggctt ccgccttcag ctcctcgccc
      901 cgctccagcg cgaacagccg aagcgaatag gccacctccc gctgctggtt catgacgtcg
      961 tcgtattcca gcagccgctt ccgctgctgg aagttctgca gctcgacccg cttctgggcc
     1021 gactcgatgg ccaccatcac cagtttgccg gtgatgactt cgccctcttc cgcgccggtc
     1081 cggtccatgt accgcgcgat gcggtccgat ccgaacagcc gcatcaggtc gtcttccagg
     1141 gacaggaaga agagcgacgc gcccggatct ccctggcgcc cggcccggcc gcggagctgc
     1201 cggtcgatcc ggcgcgactc gtgccgctcg gtgcccagga tctggaggcc ggcttcctcg
     1261 gtggtcagat cgaggtcctt gcccagtttg atgtcggtgc cccggccggc catgttggtc
     1321 gcgatggtga tcgcggcctt ctgccccgcg ccggccacaa tctccgcttc gcgctcgtgg
     1381 tacttcgcgt tgaggacctc gtgggtgagg ccccgccgct tgagcatctt ggacagggtc
     1441 tccgacacgt ccaccgacgt ggtcccgatg agaatcggaa gccccaggcc atgcagccgc
     1501 tcgatctcat cgagtaccgc gttgaatttt tcccggcggg tcttgtagat ccggtcggtg
     1561 tggtcggtgc ggcggatcgg cttgttggtg gggatgaccg ccacctcgag gccgtagatc
     1621 gagtagaact ccgactcttc cgtttccgcc gtaccggtca tgccggcgag cttgtcgaac
     1681 atccggaagt agttctggat cgtgatcgtg gcgagcgtct gggtctcgcc cttgatctgc
     1741 accgcttcct tcgcctccac cgcctggtgc aggccgtccg accaccgtcg ccccgccagc
     1801 acccggccgg taaactcgtc cacgatcagc acctggttgt cctgcaccag gtagtccacg
     1861 tctttttcgt acagcgagtg cgcctgcagc agcttgtgga tgatgtgcag tttctcgctc
     1921 ttgaccgcgt attccgcttc cacccgctgc cgttccgccc gcttttccgg aatgccgagg
     1981 ttgggatcct tctcgatctt gccgacctct tcggagatgt cgggcaccag gaacatctcc
     2041 ggatcgtccg gcgacagcgc tgcggccccg cggtcggtca ggtgcaccga gtggcccttc
     2101 tcgtcgagca cgtagtagag cacgtcctcg aggccgcgca tgcgctgctg ccgggtcggc
     2161 agcttgcggt ccgcgatgta gtccagctcg gcccgctgca ccagctgctt gacgccggtc
     2221 tcattgagca gcttgagcag ctttttgttt ttcggcatgc cgagctgggc ctgaaacagc
     2281 ttgtacccgg cctcttcctt cttcttctcg tcggggagaa tcggctcggc ttcggccatg
     2341 agctggttca ccacggcggt ctgcttgcgg accaggtcgg cgaccgggcg gttgaagatg
     2401 gtgtacttct cgtcgccctc gttaccgacc ggaccgctga tgatgagcgg agtccgggcc
     2461 tcgtcgatca ggatggaatc gacttcgtcc acgatggcat aggcatggtg gcgctggacc
     2521 cgctggtcca gtgagaacac catgttgtcg cggaggtagt cgaagccgaa ctcgttgttg
     2581 gtgccgtaag tgatgtcggc attgtaggcg tcccgccgcg ccggggagga cggctcggtg
     2641 tcgtcgatgc acccgacggt caggccgaga tacttgtaga cgtgccccat ccactgcgaa
     2701 tcgcgccgcg ccaggtagtt gttgacggtg accaggtgcg cgccgcgacc ggccagggcg
     2761 ttgagataca gcggcagcgt cgcgaccagg gtcttgcctt cgccggtggc catttccgcg
     2821 atcttgccct cgtggagaac ggcaccgccg atgagctgga cgtcgaacgg gatcatgtcc
     2881 cagcggacgt cgatgccgac gatcgaccac gatttgccga cgtggcgccg gcaggcgtcc
     2941 ttgacgacgg cgaaggcctc cggaaggatc tcctccatcc gctcgcgaac cgccgcttcc
     3001 cggtcatcag g
//
LOCUS       DASRJQ010000677         3916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1815605, whole genome shotgun sequence.
ACCESSION   DASRJQ010000677 DASRJQ010000000
VERSION     DASRJQ010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1815605"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VFB89_15305"
     CDS             <1..182
                     /locus_tag="VFB89_15305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28715.1"
                     /translation="LNRSRRIVWVVTGREKAEMLARVCSGDRSIPAGRISQEQALVLA
                     DREAAARLGPELALR"
     gene            208..672
                     /locus_tag="VFB89_15310"
     CDS             208..672
                     /locus_tag="VFB89_15310"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HZM28716.1"
                     /translation="MQVGIATDHGGFELKEGLLSRLRAAGHQVIDFGADELTPDDDYP
                     DFVIPLAYAVAAGSVERGVAVCGSGVGASICANKVSGIRAALIHDHFSAEQGVEDDDM
                     NIICIGGRTVGPSVAWELVETFLAARFSHAPRHERRLSKVARLELEATRSHR"
     gene            669..2753
                     /gene="tkt"
                     /locus_tag="VFB89_15315"
     CDS             669..2753
                     /gene="tkt"
                     /locus_tag="VFB89_15315"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HZM28717.1"
                     /translation="MSVTSVAEPITETKLDQLAVDTIRTLSIDAVQQAKSGHPGTPMA
                     LAPLVYTIWNRVMRFDPEDPIWPNRDRFVLSNGHASMLLWSILHLTGTRAVNADYERL
                     GKPSVSLDDIRRFRQLDSKAPGHPEYHWVSGVETTTGPLGQGIATSVGMAIAQKWLAD
                     RYNRPGFPLFDYNIYVVCGDGCLMEGVGSEAASLAGHLGLDNLCWVYDNNHITIEGST
                     SLAFTEDVATRFLAYRWNVLRVGDANDLDRIEQALSVFRMTTGRPTLIILDTHIGYGS
                     PHRQDTAAAHGEPLGEEEVTLTKRSYGWPEEARFLVPEGVREHFAAGIGARGAEARSK
                     WTALLASYRAAYPELATEIDQMQRRELPLGWDRNLPVFPADAKGIAGREASGKVLNVL
                     AQNVPWFLGGSADLGPSNKTTLTYEGAGHFQAGTWSGKNLHFGIREHAMAAIVNGLAL
                     SKVRAFGATFFIFSDYARPAIRLSALMELPCIFVFTHEAMGDGEDGPTHQPVEHLASL
                     RAIPGLVTLRPADANEVVEAYRYVMQLQHQPAVLALSRQPLPTLDRRTYAPATGVARG
                     AYVLADPPGGNPEVILIASGSEVSLAVKAHEQLLAEGIRSRVVSMPSWDIFEHQDLGY
                     RRSVLPPDVAARVAIEQASTFGWERYVGPSGRVIAMETFGASAPLSELQRKFGFEPGR
                     VVAAVKELVGRS"
     gene            2764..>3916
                     /locus_tag="VFB89_15320"
     CDS             2764..>3916
                     /locus_tag="VFB89_15320"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HZM28718.1"
                     /translation="MAGEGLSADAGDAARTGRSFPLGASIAGEGVNFSVFSRGASAVE
                     LLLFEREDDARPARVIPIDPAANRTYHYWHVLVPGVGAGQLYGYRVYGDWEPAGGQRF
                     DPTKVLLDPYGKGVVVPKQYSRDAARRSGDNAAWAMKSVVVDVSAYDWEGDTPLHRPS
                     ARTIIYEMHVRGFTRHPSSGVSEGTRGTYVGLIEKIPYLRQLGITAVELMPVFQFDAQ
                     DAPPGKVNYWGYAPISFFAPHQGYSSRQGPLDPVNEFRDMVKALHRAGIEVILDVVFN
                     HTAEGDHTGPTLSFRGLDNTTYYILERDRSRYANFSGTGNTLNGNHPVVRRMIVDSLR
                     YWVETMHVDGFRFDLASILDRDESGNVMASPPVLLDIESEPALAGTKLIA"
BASE COUNT          693 a         1247 c         1278 g          698 t
ORIGIN      
        1 tactcaaccg ctctcgccgc attgtgtggg tggtcacggg gcgtgagaag gcggagatgc
       61 tcgcacgtgt gtgcagcggc gaccggtcga ttcccgccgg gcgaatcagt caggaacagg
      121 ctttggtact ggccgatcgg gaagcggcgg ctcggttggg acctgagctc gcgctacgct
      181 gacgtcacat cactggaggg ggctggtatg caggtgggaa tcgcaaccga ccacggcggg
      241 ttcgagctga aagagggcct gctctcgcga ctccgcgcgg ccgggcacca ggttatcgat
      301 tttggggcgg acgagctcac cccggatgat gactacccgg acttcgtcat cccgcttgcc
      361 tacgctgtgg cggcgggctc ggtcgagcgc ggggtagccg tgtgcgggag cggcgtgggt
      421 gcctcgatct gcgccaacaa ggtgtcaggt attcgtgccg ctctgatcca cgatcacttt
      481 tccgccgagc agggcgtcga ggatgatgac atgaacatca tctgcatcgg cggccgcacc
      541 gtggggccga gcgtggcctg ggagctcgtg gagacattcc tggccgcgcg gttcagccac
      601 gccccccgac acgagcgccg cttgagcaaa gtggcgcggc tggaactcga agccacgagg
      661 agtcatcgat gagcgttacc agtgtcgctg aaccgataac cgaaaccaag ctcgaccagc
      721 tggcggtcga cacgattcgt acgctctcta tcgacgcggt tcagcaagcg aagtcagggc
      781 accccggcac accaatggcg ctcgcgccac tcgtgtacac gatctggaac cgcgtgatgc
      841 gcttcgatcc ggaggatccg atctggccca atcgtgaccg gttcgtcctg tcgaacggtc
      901 acgcgtccat gctgctctgg tccatcctgc atctgacggg aacgagggcc gtgaatgcgg
      961 actacgagcg cctggggaag ccttcagtat ccctggatga catccgtcgc tttcgccagc
     1021 tcgacagcaa ggcccccgga cacccggaat atcactgggt atcgggtgtc gagaccacga
     1081 cgggtcccct cgggcaagga atcgccacca gcgtaggaat ggccatcgct caaaagtggc
     1141 tcgccgaccg ttataaccga cccggattcc ccctgtttga ctacaacatc tacgtggtct
     1201 gcggcgacgg ttgtctgatg gagggcgtcg gctcggaagc agcctccctc gccggtcacc
     1261 ttggtctcga caacctgtgc tgggtgtacg acaacaacca catcacgatc gagggatcga
     1321 ccagcctcgc cttcaccgaa gacgtcgcga cccgcttcct ggcctaccgg tggaacgttc
     1381 tccgcgtcgg tgatgccaat gatctcgatc ggatcgagca ggcgctctcg gtctttcgga
     1441 tgaccaccgg gcggcccacg ctgatcattc tggacaccca catcggctac gggtcccctc
     1501 accgacagga cacagccgcg gctcacgggg aaccactcgg cgaggaggag gtcaccctca
     1561 ccaaacgcag ctacggctgg cctgaagagg cacggttcct ggttcccgaa ggagtccgcg
     1621 agcatttcgc ggcgggcatc ggggcgcgcg gcgctgaggc tcggagcaag tggacggcgc
     1681 tccttgcctc gtaccgcgct gcttacccag aactggccac cgagatcgat cagatgcagc
     1741 ggcgcgagct accgcttgga tgggaccgca acctgccagt cttcccggca gacgcgaagg
     1801 gtatcgcggg ccgagaggct tcgggtaagg tgctcaacgt gctcgcgcag aacgtcccat
     1861 ggttcctggg aggctcggct gatctcggcc cgtccaacaa gacgaccctg acgtacgagg
     1921 gcgcgggaca tttccaggcc gggacttgga gcgggaagaa tctgcacttt ggcatccggg
     1981 agcatgcgat ggccgcgatc gtcaacggcc tcgcactctc caaggtgcgg gcgttcggcg
     2041 cgacgttctt catcttcagc gactacgcac gccccgccat ccggctgtcc gccctgatgg
     2101 agctgccgtg catcttcgtg ttcacgcacg aggccatggg cgatggcgag gatggcccca
     2161 cgcaccagcc ggtggagcac ctggcgtcac tccgggcaat tccgggtctg gtaacgctgc
     2221 ggcccgcgga tgccaacgaa gtcgttgaag catatcgcta cgtgatgcag ctccagcatc
     2281 agccggccgt tctcgcgctc tcacggcagc cgttgccaac tctggatcgg cgcacctacg
     2341 cgcccgcgac gggcgtcgca cgtggcgcct acgtgctcgc cgacccgccg ggtggcaatc
     2401 ccgaggtcat actcatcgcc tcgggcagcg aggtgagtct ggccgtgaag gcgcacgagc
     2461 agctgctcgc ggagggcatc cgttcccgcg tggtctcgat gccgtcgtgg gacatattcg
     2521 agcaccagga tctcggttat cgaaggagcg tgctgccacc cgacgtggct gcccgcgtag
     2581 cgatcgagca agcctccacc ttcggatggg agcggtacgt cggcccgtcc ggccgggtga
     2641 tcgcgatgga gactttcggg gcttcggcgc cgctcagtga gctgcaacgg aagttcgggt
     2701 tcgagccagg tcgggtcgtt gcagcagtga aagagctggt gggcaggtcg taaggagtgg
     2761 gcggtggcgg gcgaaggact ctcagccgat gcgggcgatg ctgctcgcac cgggcggagc
     2821 ttccctctcg gggcgtcgat cgccggtgaa ggcgtcaatt ttagcgtctt ctcacggggc
     2881 gcctctgccg tcgagttgct gctctttgag cgagaggacg atgcccgccc cgcccgggta
     2941 attccgatcg accccgccgc aaaccgcacg taccactatt ggcacgtctt ggtccccggc
     3001 gtcggtgccg gccagttgta cggctaccgg gtgtatggag actgggagcc ggcgggcggg
     3061 caacgcttcg accccaccaa agtactcctc gatccctatg gcaaaggcgt agttgttccc
     3121 aagcagtaca gccgcgatgc tgcgcgccgg agcggcgata acgctgcctg ggccatgaag
     3181 agcgtggtgg tcgacgtctc agcgtacgac tgggagggcg acacccccct gcaccggcct
     3241 tcggcgcgga ccatcatcta tgagatgcac gtgagaggat tcacccggca tcccagctcc
     3301 ggcgtttcgg aaggaacccg cggtacctac gtcggcttga tcgagaagat cccctatctc
     3361 cggcagctag gcatcaccgc tgtcgaattg atgccggtat tccagttcga cgcccaggat
     3421 gcgccgccgg ggaaggtgaa ctattggggc tatgcaccga tctcgttctt cgctccgcac
     3481 cagggttaca gctcccgtca gggcccgctc gacccggtga acgagtttcg cgacatggta
     3541 aaggcgctgc accgggccgg catcgaagtg attctcgacg tggtcttcaa tcacaccgcc
     3601 gagggcgacc acaccggccc cacactgagc ttccgcgggc tcgacaatac gacctactac
     3661 atcctcgagc gggaccgctc gcggtacgcc aacttcagcg gtaccggcaa cacgctcaac
     3721 ggcaatcatc ccgtggttcg ccggatgatc gtcgacagcc ttcggtattg ggtcgagacg
     3781 atgcacgtgg acggcttcag gttcgacctg gcgtcgatcc tcgatcgaga cgagtcaggc
     3841 aacgtgatgg cgagtccacc cgtgctcttg gatatcgagt cggagccggc gctggcaggg
     3901 acgaagctca tcgccg
//
LOCUS       DASRJQ010000678         4039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1534594, whole genome shotgun sequence.
ACCESSION   DASRJQ010000678 DASRJQ010000000
VERSION     DASRJQ010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1534594"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3025)
                     /locus_tag="VFB89_15325"
     CDS             complement(<1..3025)
                     /locus_tag="VFB89_15325"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZM28719.1"
                     /translation="MALEHIHSNPVGVYGDVLLDRGIEVHRVRLDLGEALPDWREYDL
                     LVVMGGAMSVYEEDTYPWLVGEKRAIREAVTAGCPYFGVCLGSQLLASALGSRVFRGP
                     EPELGVNPIFLCEAARRDPVFRAFPPDVEVFEFHSDTFDLPDGAVRLARSSRYENQAF
                     HVGRTAYAIQCHLEPSLDDARDWFDAPSLAETFETRYGRGSVAGFLEEYARSMPLLQQ
                     TARQLFRRWLENALAHGRPTSAASGVRTATLSERGLLGRAREQELIGRLLDQARAGRS
                     GAIVICGETGLGKTALLDDAVQNADGMRVLRVSGVEDVSERPYAGFEELCRPLLGGLG
                     ALPDTLRQALSATLGLTLAERGDRFAAFTGALWLLATAAGEEPLLVCVDDAHLLDDAS
                     LEALAFIAGRIGAEGIALLVATGGDPGTFGAVEVVELQPLDRAASLTLLEQTFGNELS
                     PTVIAEVVNASRGNPLALREIPLSLTPNQRFGSAPLGDALITCRSAERAILARFSALG
                     VEARRALLVVALSYGEDGAAITRALQSAHLSLEELRPAQEAGLVAISGGRFSFPHPLV
                     RSTVVYGALRTERRAAHAALAGASNSDSSTWHAALAAPGPDERIAASLEDAAGRALSR
                     HAHSAAARALELAARLSPARAKRARRLVSAAEAAALAGHIYAAVDHVEAALPELDDGE
                     ARAAAELLLGNLLARSGSAPRARDLLLDGAARCEASYRSAAARLLAAAVIPALRAGSP
                     ARACEIGQRALELAKGGPIEAAASLVLGTALTFNGDLCKGRTLLLRSIELRDQLQAEP
                     QLRAYLGAGLRVAGEHDRARDVLAQLIAEARSHGAFGLLPYALVRLADVELDTGHWRA
                     ANAALAEAASLAREMGQGADQGLASGALAWLDAAQGRAVDCRRHASEAIALAVRLGVG
                     SRLDRAVPALGLLALGCGELEAAIEHLAEVRKTQLEQGWCDAAVSPHRARDLIEAFVR
                     AGRVADAEREVSIFEHETRQTERPTALAALACCRGL"
BASE COUNT          715 a         1345 c         1305 g          674 t
ORIGIN      
        1 gcagtccgcg gcagcaagcc aaggccgcga gcgccgtagg ccgttccgtc tggcgggttt
       61 catgctcgaa gatcgaaacc tcgcgctccg cgtcggcgac tcgcccagcg cgaacgaacg
      121 cctcgatcag gtcgcgcgcg cgatggggag acacagccgc gtcgcaccag ccctgttcga
      181 gctgcgtctt acgtacctcg gctaagtgct cgatggcggc ttccagttca ccgcagccga
      241 gcgcgagaag gcctagcgcg ggaaccgcac ggtcgaggcg tgagccgact cccaagcgca
      301 cagcgagggc aatcgcctcg ctggcatgcc ggcggcagtc gacggcacgt ccttgtgcgg
      361 cgtctagcca ggctagcgca cccgaagcga ggccctggtc agcgccttgc cccatctcgc
      421 gtgctagcga agcggcctct gcgagggcag cattcgccgc ccgccagtga cccgtgtcga
      481 gctcgacatc ggctagacgc actagcgcgt agggaagcag gccgaatgcg ccgtgagaac
      541 gcgcctcggc gatcagttgt gcgagcacgt cccgagcacg gtcgtgctcg ccggcgaccc
      601 gaagacccgc gcctaggtag gcgcgcagct ggggctccgc ctgaagctgg tcccggagtt
      661 cgatggatct gaggagaagc gtacggccct tgcacaaatc gccgttaaac gtcagcgccg
      721 tgccgagcac gagcgacgca gcagcttcga tcggtccgcc cttggcgagc tcgagtgccc
      781 gctgtccgat ctcgcaggcg cgcgctggcg acccggcgcg gagtgccggg attaccgccg
      841 cagcaagcag acgggccgcc gccgacctat aggacgcctc gcagcgcgca gcgccgtcga
      901 gcagcaggtc gcgtgcccgc ggggccgagc cgctgcgcgc gagtaggttg ccgagcagca
      961 gttcggccgc cgcccgcgcc tccccatcgt cgagctcggg gagggcagcc tcgacatggt
     1021 cgactgccgc atagatgtgg ccggcaagtg cggccgcctc agccgcgcta actagccgtc
     1081 tcgcgcgctt ggcccgtgca gggctcaggc gtgccgcgag ctcgagcgcg cgcgcggccg
     1141 ccgaatgtgc gtgccgtgaa agcgctcgcc cggcggcgtc ctcaagggaa gccgcgatcc
     1201 gctcgtcggg gcccggggcg gcgagcgctg catgccatgt agacgagtcg gagttgctgg
     1261 caccggcgag ggcggcgtgg gcggcacgcc gctctgtacg aagtgctcca tagaccacag
     1321 tcgagcgcac gagcggatgc gggaaagaga agcggccgcc cgaaatagcg acgagtccag
     1381 cctcttgggc cggtctcaat tcctcgaggc tcaagtgagc cgattggagc gcccgcgtga
     1441 tggccgcgcc gtcctccccg tagctgagcg ccaccacgag cagagcccgg cgcgcctcca
     1501 ccccgagcgc gctgaagcgg gcaaggatgg cccgctccgc ggatctgcaa gtgatgagcg
     1561 cgtcgccgag tggggcgctc ccaaagcgct ggttgggcgt gaggctgaga gggatctcgc
     1621 gtagtgcgag cgggttgccg cgcgaggcgt tgacaacctc agcgatcacg gttggtgaca
     1681 gctcgttgcc gaaagtctgc tcgagcaggg tcagtgacgc tgctcggtcg aggggctgaa
     1741 gctcgacgac ctcaactgcg ccaaatgttc ccgggtcacc gcctgtcgcc acgagcagcg
     1801 cgatcccttc cgctccgatc cgtcctgcga tgaacgccaa cgcctcgagc gaggcgtcgt
     1861 cgagtaggtg cgcgtcgtcg acgcaaacaa gaagtggctc ctcccctgcc gctgtggcaa
     1921 gtagccacag cgcgccagtg aacgcggcga accgatcgcc gcgctcggca agggtgaggc
     1981 cgagggtcgc cgacagagct tggcgaaggg tgtctgggag ggcgccgagc ccgccgagca
     2041 gcggtcggca tagttcctcg aaccctgcgt acgggcgctc actcacgtcc tcgacaccgg
     2101 aaacccggag aacgcgcatc ccgtcggcgt tctggaccgc gtcgtcgagt agcgctgtct
     2161 ttcccagccc ggtctcaccg cagatcacaa tcgcaccgct gcggccggcc cgcgcctggt
     2221 cgagcaaccg accaatgagc tcctgctcac gggcacgacc aagtagcccg cgctccgaga
     2281 gggtggccgt tctcacgccg cttgcagccg aggtcgggcg gccgtgggcg agtgcattct
     2341 ccagccagcg ccggaacagc tgacgggcgg tctgctggag tagcggcatg gagcgcgcgt
     2401 actcctccag gaagccagcg acggagccac gtccgtaacg ggtctcgaac gtctccgcga
     2461 gcgagggcgc gtcgaaccag tcacgggcat cgtcgagcga gggctccaga tggcactgga
     2521 ttgcatacgc cgtccgcccg acatggaacg cctggttttc gtagcgggag gagcgggcga
     2581 ggcgcaccgc gccgtccggc aaatcgaatg tgtcggagtg aaactcgaac acctcgacgt
     2641 cgggtggaaa ggccctgaag accgggtcgc ggcgtgcggc ctcacagagg aagatcggat
     2701 taacgcccag ctcgggctcc ggcccacgga acacgcgcga gccaagcgcg gacgccagca
     2761 gttgggatcc taagcaaacg ccgaagtatg gacatcccgc cgtgactgcc tcgcggatcg
     2821 cccgcttctc ccccacgagc cacgggtacg tgtcctcctc gtagacgctc atggcccctc
     2881 ccatgaccac gagtaggtcg tactcgcgcc agtcgggaag cgcttcccct aggtcgagac
     2941 gaacacgatg gacctcgatc ccgcgatcga gaagcacgtc accgtagacg cccacgggat
     3001 tcgaatggat atgctcaagc gccactgccc gcatgggtga tcgagcccgc ctggtgcgca
     3061 atgatcagac ttaaactata cacctggctg acaattttct tccgtcaatc tgcgcactat
     3121 atctcttccg ggctgtgcct tctcccctgg tatctccggc gctcgcatga aggcgtcgcc
     3181 aattgggcaa acgaccgacc aaacttcgcc gcgctagagg ttgctaggcg caataaaaga
     3241 aattagcctg agagccattt attccatctc tgggacaggc gcgtcgtcca atggcccgat
     3301 ttttgttcag gccaatttaa ctcccgctac ttctagacct cttctcactc ccattagaaa
     3361 gggagtcatg tttccaggtg tgtcgggctg cctcggtatg tctggggttg gtgcctccgt
     3421 ccctttcaca gcttggtacc caacgttgaa accagccctg aaattatcgt gtggcactcc
     3481 gctcggaatc gtccaatctg gcatgacaaa tcctcctccc gccacatgct ccagcacgat
     3541 ataagccttc tttccttctt tcgatctcta gtttttactg tcttacagta attgaccatt
     3601 ggtgtgtgtc tgagcgaggg cctagtcccc caggcccgct tcattagtgc gagcggtctg
     3661 actagcccta cccgatcttg ccattggagc cggtccgtcg ctttgcgcgc tgacaccctc
     3721 atcccttcga ggatggcctt cagtgttccg ctagcagtta cttcagcacg tcagaacaaa
     3781 cgccggtttg aggtgcgacg gtccacctat ccgcgcagtg gtacaggcac cggtgataat
     3841 tgagccagca gtggcgcgac cggcggccga gcagtaaaat gaaactcgct gaacttgaca
     3901 gtccaccatg cgcgccggac gcctgctcat aggtcctctg ctgcggcggg tggttggcac
     3961 ccgggcgact gtatgggtcg aaaccagcgc gcccgcggtg gtcaccgtcc gcgcggccaa
     4021 cggcgcctca ggcaccgcg
//
LOCUS       DASRJQ010000679         3727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3705687, whole genome shotgun sequence.
ACCESSION   DASRJQ010000679 DASRJQ010000000
VERSION     DASRJQ010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3705687"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VFB89_15330"
     CDS             complement(<1..693)
                     /locus_tag="VFB89_15330"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HZM28720.1"
                     /translation="MTDADRLDQLCINTIRTLSIDAVQKASSGHPGTPMDAAPTAYCL
                     WQRFLRYDPADPRWLNRDRFILSNGHASMLLYSLIHLADVQAANPSYETLGRPAVTLD
                     DIKTFRQAGSRCPGHPEYGWTSGVEATTGPLGQGVAMSVGIAMAGRWLAATYNRPGYD
                     LFDYNVYALAGDGCMMEGVSGEAASLAGHLGLANLCWIYDDNRISIEGPTSLAFTEDV
                     AARFLAYGWNVTR"
     gene            complement(690..2336)
                     /gene="pgi"
                     /locus_tag="VFB89_15335"
     CDS             complement(690..2336)
                     /gene="pgi"
                     /locus_tag="VFB89_15335"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HZM28721.1"
                     /translation="MTAVAASAGTSLTQRPAWRALESHYRSIRDVHLRDLFARDSERG
                     ERLSAEAAGVFLDYSKHRITDETLRLLLELARECGLRERIDAMFAGEAINETEHRSVL
                     HIALRAPRSESIVVDGRNVVPEVHQVLDRMSAFADRIRSGEWKGHTGKPIRNVINIGI
                     GGSDLGPVMAYEALRHYSRRDLTFRFVSNVDGTDFAEKTNGLDPAETLFVVSSKTFTT
                     LETMTNARSAREWLVARLGDAAVAAHFVAVSTNATEVAKFGIDTGNMFGFWDWVGGRY
                     SLESAIGLSTMIAIGSDHFRAMLAGSHAMDLHFRTAPFEQNLPVLMGLLAVWYADFFG
                     AETVAVLPYDQYLVRFPAYLQQLTMESNGKHVTLDGRRVDYLTGPIYWGEPGTNGQHS
                     FYQLLHQGTRLVPADFIGFYRSLNPLGAHHDLLMANLFAQTEALAFGKTADEVRSEGT
                     PAWLEPHRVFEGNRPSSTILAQQLTPEVLGALITLYEHSTFTQGAVWQIDSFDQWGVE
                     LGKVLAMRIVPELAPDAGTTAHDSSTSRLIQLYRQFRRAP"
     gene            complement(2333..3436)
                     /gene="tal"
                     /locus_tag="VFB89_15340"
     CDS             complement(2333..3436)
                     /gene="tal"
                     /locus_tag="VFB89_15340"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HZM28722.1"
                     /translation="MNPTAQLHELGQSLWLDNITRGLLTSGTLRRYLADFSVTGLTSN
                     PTIFDQAISHSAFYDEAIRRKAAEGKSGEALFFDLALEDLGQAADLFRPIHEETRGMD
                     GWVSLEVSPLLAYDTKSTIEEAKNLHARGRPNLFIKIPGTEKGLPAIEESIFAGVPIN
                     VTLLFSREQYLAAAEAYMRGIERRLAAGLDPRVRSVASLFVSRWDKAVAGKTPEDLRG
                     RLGIAVAGRTYRAYRELLSSARWQRLAREGAAPQRLLWASTGTKDPKLSDMLYLVALA
                     APDTINTMPEATLLAFADHGMLGEVMRPDGGDAEAVLGRVGQAGIDVDALAEQLQQEG
                     AEAFVASWKDLLSCIESKAAALRDPALAGESRR"
     gene            complement(3433..>3727)
                     /locus_tag="VFB89_15345"
     CDS             complement(3433..>3727)
                     /locus_tag="VFB89_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28723.1"
                     /translation="IYVHKEQELADVERFYPADHYVLIDDKVRILTAVKQAWGSRVTT
                     IFVRQGHYAHDPAVLAEYPPADQSVAAIGDLLHLDPHVLLEAGTAGGTTGRII"
BASE COUNT          687 a         1180 c         1183 g          677 t
ORIGIN      
        1 ccgggtcacg ttccagccgt acgccagaaa gcgcgcggcc acatcctcgg tgaacgcgag
       61 ggaggtgggt ccttcgatgg aaatgcgatt gtcgtcgtag atccagcaaa ggttggcgag
      121 accgagatgt cccgccagcg atgccgcttc gccgctcacg ccttccatca tgcatccgtc
      181 gccggcgagc gcgtacacgt tgtagtcgaa caggtcgtac ccaggtcgat tataggtcgc
      241 ggccagccat cggccagcca tggcgatccc gacactcatg gcgaccccct gccccagtgg
      301 gcccgtggtc gcctccactc ccgaggtcca gccgtactcc gggtggcccg gacagcggct
      361 gcctgcctga cgaaacgtct tgatgtcatc cagcgtcaca gccggacgtc cgagcgtttc
      421 gtaggaggga ttggccgcct gaacgtccgc gaggtggatc aggctgtaga gcagcatcga
      481 ggcgtgacca ttcgacagaa tgaaccgatc tcgattgagc cagcgggggt ctgcggggtc
      541 gtaccgcaga aatcgctgcc agagacagta cgccgtcggg gcggcgtcca tcggcgtccc
      601 gggatgaccc gaactggctt tctgcacggc atcgatggac agcgtgcgga tggtgttgat
      661 acagagctga tcgagccgat cggcatcggt catggtgccc tccgaaactg ccggtacagc
      721 tggatcaacc ggctggtgga gctgtcgtgg gccgttgtcc cggcgtcagg agccagttcg
      781 ggcacgatcc gcatcgccag taccttgccc aactccacgc cccattgatc gaacgaatcg
      841 atctgccaga cagcgccctg ggtgaacgtg ctatgctcgt acaacgtgat cagcgcgccc
      901 agaacttccg gcgtcagctg ctgggcgaga atggtgctac tgggccggtt gccctcgaac
      961 acgcggtgcg gctcgagcca ggccggcgtt ccctccgagc ggacctcatc ggccgtcttc
     1021 ccgaaggcca gcgcctcggt ctgcgcaaag agattggcca tgagcagatc gtggtgcgct
     1081 ccgagaggat tgagcgaccg atagaagccg ataaagtcgg ccggcacgag acgggttccc
     1141 tgatggagta gctggtagaa ggagtgctgg ccgttagtcc ccggctcacc ccagtagatg
     1201 gggccggtga gataatccac ccggcgcccg tccagggtga catgcttccc gttgctctcc
     1261 atagtgagct gctgcaaata ggccgggaat cgcactaggt attgatcata gggcagaact
     1321 gcaaccgtct ccgcgccaaa gaagtcggca taccagacgg ccagcagtcc catgagcacg
     1381 ggcaggtttt gctcgaacgg tgcggttcgg aaatggagat ccatcgcatg cgagccggcc
     1441 aacattgcgc ggaagtgatc cgatccgatc gcgatcatgg tcgagaggcc gatggccgac
     1501 tcgagcgagt atcgcccccc cacccaatcc caaaatccga acatgttgcc ggtgtcgatc
     1561 ccgaactttg ccacttcggt ggcattggtg gagacggcca cgaagtgcgc ggcaaccgca
     1621 gcgtcaccga gccttgccac cagccactcg cgagcgctgc gcgcgttggt catggtctcg
     1681 agcgtggtga acgtcttgga ggagaccacg aacagcgttt cggccgggtc gaggccgttg
     1741 gtcttctctg cgaagtcggt tccgtccacg ttcgacacga agcggaacgt gaggtcgcgg
     1801 cggctgtagt gtcgcaacgc ttcgtacgcc atgactgggc cgagatcgga tccgccgatt
     1861 ccgatattga tgacgttgcg gatcggcttg ccggtatggc ccttccattc gccactacgg
     1921 atacgatcag cgaaggcgct catgcgatcg agcacctggt ggacctcggg aactacgttg
     1981 cggccgtcca ccacgatcga ttcgctccgc ggcgctcgga gcgcgatatg cagcaccgac
     2041 cggtgctcag tttcgttgat cgcttcgccc gcaaacatgg cgtcgatccg ctcgcgcagg
     2101 ccgcactcgc gagccagctc cagcagcaga cgcagagttt cgtctgtgat ccgatgcttg
     2161 gaatagtcga ggaaaacgcc ggccgcttcc gcgctcaacc gctctccccg ttcagagtcg
     2221 cgagcaaaga gatcccgcag gtgaacgtcg cgaatgctgc gatagtggct ctcgagcgcc
     2281 cgccaggcag ggcgctgcgt gagagacgtg cccgcagatg cggccaccgc agtcatcgac
     2341 gcgattctcc cgccagcgcc ggatcccgca gcgcggcagc cttcgactcg atacaactga
     2401 gcagatcttt ccatgatgcc acaaatgcct cggcaccctc ctgctgaagc tgttccgcga
     2461 gcgcgtccac gtcgatgccc gcctggccga ctcgacccag caccgcctcc gcgtcgccgc
     2521 cgtcgggccg catgacctcg ccaagcattc cgtgatcggc gaaggcgagg agcgtggctt
     2581 cgggcatggt attgatcgtg tcgggcgcgg cgagggcgac aaggtagagc atgtcggaca
     2641 gcttggggtc cttggtgccc gtgctggccc agaggagacg ctggggagcg gccccctcac
     2701 gcgcgagccg ttgccagcgc gcggagctga ggagctctcg ataagcccgg taggttcgcc
     2761 cggcgacggc aatccccagg cgtccccgca ggtcctccgg tgtctttccc gcgactgcct
     2821 tgtcccagcg gctcacgaac aacgaagcca ccgatcgcac tcgggggtcc agcccggctg
     2881 cgagtctccg ctcgatacct cgcatgtacg cctcggccgc cgccagatac tgctcccgcg
     2941 agaaaaggag cgtgacgttg ataggcaccc ccgcaaagat cgattcctcg attgcgggca
     3001 gccccttctc agtgcctggg atcttgatga aaagattcgg gcgaccgcgg gcgtgcaggt
     3061 tcttggcttc ctcgatcgtg ctcttcgtgt catacgccag cagcggagat acttccagcg
     3121 acacccaccc atccataccc ctggtctctt cgtgaatcgg acggaagaga tcagccgcct
     3181 ggcccaggtc ctccagcgcg agatcgaaga acagcgcctc gcccgacttg ccttcggccg
     3241 ctttgcgacg aatcgcctca tcgtaaaacg ccgagtggct gatggcctgg tcgaagatgg
     3301 tggggttgga cgtgaggccg gtgacggaga agtcggcaag gtaacgtcgc agcgtaccgc
     3361 tggtgagcag cccgcgggta atgttgtcaa gccagagact ctggccgagt tcgtggagtt
     3421 gtgctgtggg attcatatga tccttccagt cgtgccaccg gccgtgccgg cctcgagcag
     3481 cacgtgtggg tccagatgca gcaagtcgcc gatcgccgca acgctctgat ccgccggcgg
     3541 gtattcggcg agcacggcag ggtcatgggc ataatggccc tgccggacaa aaatcgtggt
     3601 gacgcgtgag ccccaggctt gcttcacggc cgtgaggatc cggaccttat catcgatcag
     3661 gacgtagtga tccgccggat agaatcgctc gacgtcggcc agctcctgtt ccttatggac
     3721 atatatc
//
LOCUS       DASRJQ010000680         4321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1252175, whole genome shotgun sequence.
ACCESSION   DASRJQ010000680 DASRJQ010000000
VERSION     DASRJQ010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252175"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VFB89_15350"
     CDS             <1..262
                     /locus_tag="VFB89_15350"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZM28724.1"
                     /translation="DLTEKMAFFEAYGAIAGPPALKTLSEMLLPRGLLKLRQASETRA
                     CAAIALGKIRTPEAREILTRAAEDKDLVVRNAVNRALRESAA"
     gene            259..1734
                     /locus_tag="VFB89_15355"
     CDS             259..1734
                     /locus_tag="VFB89_15355"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZM28725.1"
                     /translation="MTSAPEPTRPQPDGAAAQDARLRQAGRGLLLELYAALRSLKLYP
                     LENATVQKALYDLEASAEKLIQAEGDIEVRLAGDFIFVNATRLRLELDNYASFSHILS
                     MFRTFEIGTLRVLPGANRLEWQIFLSLLLSLSSRSGTEDLFTELQRRLAEGNVRSLEL
                     ERSLPQQEGLRESEEAREVAKRTYSQGVAVTRDVITGIRLGRATSVKRVKRAVQMIVD
                     QVLNNETSLMGLTTIRDYDEYTFTHSVNVCIFAVALGKKLGFSKLQLYDLGMTALLHD
                     VGKARVPVEILNKTAGLDEGEWQIMQAHPWLGALTLFGLRGYDEIPYRSILVAYEHHM
                     KQDLTGYPRSVRPRNLGLFSRIVSVADGFDAATTRRTYQTVPIEPDQVLREMWENPKR
                     GYDTVLVKALINLIGVYPVGTCVILDTFEVAIVAATNPEPTQLNRPLLRIVISSEGAA
                     IPPPGTPVNLAERDGNGAYVRSIVKVTNPSRYGLTVGDYFV"
     gene            1727..2521
                     /gene="trpA"
                     /locus_tag="VFB89_15360"
     CDS             1727..2521
                     /gene="trpA"
                     /locus_tag="VFB89_15360"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HZM28726.1"
                     /translation="MSESRLDITWAQLRAEGRSALIPYLTAGYPTIASSLAALRAATE
                     VADIIEVGVPFSDPLADGPTIQRSTFEALRQGMTLKGTLDLIGEADLAKPVVVFSYLN
                     PVLSYGLERFLHDSAQLGIAGLLLTDLPAGSDPGIESSVAQSSLDLIRLIAPTTRPER
                     LTLAVAGATGFIYLVARLGVTGASTALAPDLPGTINAVRAATQLPIAVGFGISTPAQA
                     ATVATLADGVVVGSALVEIVGSHGVGAAVEFLRGMREAMDGAEARR"
     gene            2518..>4321
                     /locus_tag="VFB89_15365"
     CDS             2518..>4321
                     /locus_tag="VFB89_15365"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZM28727.1"
                     /translation="MTIRMSRLLPRYARVLLVGGVLLGAVALLLDRRWISLPIPTGAL
                     LAMVIALRATPVRLSKYSYLTQSGIPALVGAVTIGPGPVVVALGAGTMLADVLWVRRP
                     WRAAWINTGREIIAFVSAFGFYALALRLSNVTELSVDFLPAAFTLATVYFLETRGLFY
                     FTLMVRDKLESAEQILILRWEIISYLLTLVGAIIVVAAVHLLAPAGWVAVLLVLGGLG
                     LLTKRILEEAIAAEDLNKVHQLESTVLGNAQLSESFTQIERLAHRLLDWGDFRIYRVT
                     EAAPVLAYRGTFGRPNRGDPSLVIEPIRRQVLLEQTPALVKDTAHDSRIDAPIPDVRS
                     VLVYPIRFGDEVLGTIELEHHKRQTYGSRDLVTMATITTHVATAIHIAELRRPLVSTV
                     DQIGVQVGGLARVTDALRTTAAALAEASLAMRDGMAQQSSAAAQGLEATTTLARLSRN
                     MALEGQRSADASQRASEVADRNRAVIAGAVNRLVELKRFVGDSSSQVTALGGVTRRIT
                     GFMSTIREIADLTNLIALNAAIEAARAGREGRGFAVVADEVRALAVQALEVTRESSAL
                     LGDIATRMAGVAQQMERGQEAVADVEELSAAAAEA"
BASE COUNT          756 a         1382 c         1424 g          759 t
ORIGIN      
        1 cgacctcacc gagaagatgg cgttcttcga ggcgtatggc gcgattgccg gcccgcccgc
       61 gctcaagaca ctcagtgaga tgctgttgcc gaggggactg ctcaagctgc gacaggcctc
      121 tgagacgcgc gcctgcgccg ccattgcgct cgggaagatt cgaaccccgg aggcacggga
      181 aatcctcacg cgtgcggccg aggacaagga tctggtagtg cgcaacgccg ttaaccgcgc
      241 cttgcgcgaa tcggccgcgt gacatccgcg ccggagccaa ctcgacccca accggacggc
      301 gcggcagcac aggacgcccg cctccgccag gcaggccggg gattgctgct cgagctttac
      361 gcggcgcttc gcagtctcaa gctctacccg ctggagaacg ccacagtaca aaaggcgctc
      421 tacgatctcg aggcttcggc cgagaagctg attcaggcgg agggcgatat cgaggtgcgc
      481 ctggccggcg acttcatctt cgtaaacgcg acccgccttc gtctcgagct cgacaactac
      541 gcctcgttca gtcacatcct ctcgatgttc cgcacgttcg agatcggcac cctcagggtg
      601 ctgcccgggg ccaatcgcct ggagtggcag atcttcctca gtctcctgct cagtctatcg
      661 tcccggtcag gcaccgaaga cctgtttacc gagctgcagc gccgcctggc cgaaggaaat
      721 gtgcggagtc tggagctgga acggtcgcta ccgcagcagg aaggcctgcg ggagtcggaa
      781 gaagcgcgcg aggtcgccaa gcggacttac agccaggggg tggcggtcac ccgcgatgtg
      841 atcaccggta ttcggctcgg ccgggcaacc agcgtaaaac gggtcaaacg tgcggtccag
      901 atgatcgtgg atcaggtgct gaacaacgag acatcgctga tggggttgac cacgatccgt
      961 gactacgacg agtacacgtt cacgcattcg gtcaacgtgt gcatcttcgc ggtcgccctc
     1021 ggcaagaagc tcggattttc caagctgcag ctctacgatc tggggatgac ggcgctgctc
     1081 cacgacgtag gaaaggcgcg cgttccggtc gagattctga acaagaccgc aggacttgac
     1141 gaaggcgagt ggcagatcat gcaggcgcac ccctggctcg gggctctcac cctgttcggt
     1201 ctccgggggt atgacgagat tccctatcgg tccattctgg tggcatacga gcaccatatg
     1261 aagcaggacc tcaccggcta tccccggagc gttcggccgc gcaacctcgg cctgttctcg
     1321 cggattgtgt ccgtagcgga cgggttcgat gccgcgacca cgcggcggac gtatcagacg
     1381 gttccgatcg agcccgatca ggtgcttcgc gagatgtggg agaatcccaa gcgggggtac
     1441 gacacggtgc tggtgaaggc actgatcaac ctcatcggag tctatccggt ggggacctgc
     1501 gtcattctcg acaccttcga ggtcgccatc gtggcggcga ccaaccccga acccacccag
     1561 ctcaatcgcc cgctcctgcg gattgtgatc agcagcgagg gtgctgcgat tccgccgccc
     1621 ggcacgccgg tgaatctcgc cgagcgggac ggcaacggtg cctacgtacg gtccatcgtc
     1681 aaggtgacca acccgagccg ttacggactt acggtcggcg actactttgt ctgagtcccg
     1741 cctcgacatc acctgggccc agctgcgcgc cgagggcaga agcgcgctga ttccgtacct
     1801 cactgccggc tatcccacga ttgcgagcag cctggccgcg ctccgggccg ccaccgaagt
     1861 tgccgacatc atcgaggtag gtgtgccgtt cagcgacccg ctcgccgacg gaccgaccat
     1921 ccaacggtca accttcgagg cgctgcgtca gggcatgacg ctcaagggga ccctcgacct
     1981 gatcggcgag gcggacctcg cgaagcccgt cgtggtgttc agctacctca atcccgtgtt
     2041 gagctacgga ctcgagcgct ttctccacga ttcggcgcag ctgggcatcg ccggattatt
     2101 gctcacggat ctgccggctg ggagcgatcc aggcatcgaa agcagcgtgg ctcaaagctc
     2161 gctcgacctc atccggctta tcgcccccac cacacgcccg gagcgactga ccctggcggt
     2221 ggcaggtgcc acgggcttca tctatctcgt ggctcgcctc ggagtcacgg gagcgagtac
     2281 ggcgctcgcc ccggatttgc cgggcaccat caatgcggtg cgagccgcca cgcagctgcc
     2341 aattgcggtg ggcttcggca tctcgacccc ggcccaggca gcgaccgttg cgaccctcgc
     2401 ggacggcgtc gtggtcggga gcgccctggt ggagatcgtg ggatcgcacg gagtgggagc
     2461 ggcggtcgaa tttctccgag gaatgcgcga agccatggat ggtgcggagg cacggcgatg
     2521 acaatccgga tgtctcgtct cttgccgcgc tacgcccgag tcctcctggt cggcggcgtg
     2581 ctgctcggag cagtcgctct tctgcttgac cggcgctgga tcagcctgcc gattcctacc
     2641 ggggcgctgc tcgcgatggt tatagcgctg cgagccaccc cggtgcgtct cagcaagtac
     2701 tcctacctca cccaaagcgg gattccggca ctagtcggcg cggtgaccat agggccggga
     2761 ccggtggtcg tcgcgctcgg cgccggcacc atgctggccg atgtcttgtg ggtccgccgc
     2821 ccgtggcggg cggcctggat caataccggc cgcgagatca tcgcttttgt gtccgccttc
     2881 ggcttctatg cgctggcact ccggctcagc aacgtgaccg agctatcggt ggacttcctc
     2941 cccgccgcgt tcacgctggc aacggtctac ttcctcgaga ctcgaggatt gttctacttc
     3001 accctgatgg ttcgggacaa gctcgagtct gcagagcaga tcctgattct ccgctgggaa
     3061 atcatttcgt atctgctcac gctggtcggt gccataatcg tggtggctgc ggtacacctg
     3121 ctcgctccag cgggctgggt cgcagtgctg ctggtgctcg gcggactcgg gttgctgacc
     3181 aagcgcattc tggaagaggc aatcgcggct gaagacctga acaaggtcca ccagctcgag
     3241 agcaccgtgc tggggaacgc gcagctcagc gaatcgttca cccagataga gcggctggcg
     3301 caccggctgc tcgactgggg agacttcagg atctaccggg tgacggaggc ggcgccggtc
     3361 ctcgcgtacc gcgggacatt cggccggccc aatcgggggg atccctcact cgttatcgag
     3421 ccgattcggc gacaggtcct gttggagcag acaccggcgc tggtcaaaga caccgctcat
     3481 gactcccgca tcgacgcacc gatccccgac gtgcgcagcg tcctggtcta tcccattcgg
     3541 ttcggagacg aggtactggg cactatcgag ctggaacatc acaagcggca aacctatggt
     3601 tcgcgggacc tggtcacgat ggcgaccata accactcacg tcgccaccgc aatccacatt
     3661 gccgagctgc ggcgcccgct ggtgagcacc gtggatcaga tcggggtcca ggtcgggggc
     3721 ctcgcccggg tcaccgatgc attgcgcaca accgccgctg cgctggcgga ggcctcacta
     3781 gccatgcgcg acggcatggc ccagcagagc tcggccgcgg cacaaggact cgaggcgacg
     3841 accaccctgg cgcggctatc gcggaacatg gcgctcgagg gccagcgctc ggcggacgca
     3901 agtcagcgcg cgtcggaagt ggcggatcga aaccgcgcgg ttattgcggg ggccgtcaat
     3961 cggctggtgg aactgaaacg tttcgtcggc gacagctcga gtcaggtgac cgcgctcgga
     4021 ggggtcaccc gtcggattac gggcttcatg agcacgatcc gggagattgc cgacctgacg
     4081 aatctgatcg cgctcaacgc tgcgatcgaa gcggcccggg cggggcggga gggtcgcggg
     4141 ttcgccgtgg tcgcggacga ggtgcgtgca ttggcggtgc aggcgctcga ggtcacgcgg
     4201 gagagctccg cgctgctggg cgacatcgca acccggatgg ccggcgttgc gcagcagatg
     4261 gagcgcggtc aggaagcggt ggcagatgtc gaggagttga gtgcggcagc cgctgaagct
     4321 c
//
LOCUS       DASRJQ010000681         2289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_688668, whole genome shotgun sequence.
ACCESSION   DASRJQ010000681 DASRJQ010000000
VERSION     DASRJQ010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688668"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..698
                     /gene="sepF"
                     /locus_tag="VFB89_15370"
     CDS             192..698
                     /gene="sepF"
                     /locus_tag="VFB89_15370"
                     /inference="COORDINATES: protein motif:HMM:NF016363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HZM28728.1"
                     /translation="MAERLKRAAAWLGLVTDDRYADYDDVVDLEDLTEDSADEPLGTP
                     DKPATVTPLESRRPVPVAAPAGPTLAPHPRTADMSRIMTVHPRTYNEARIVGEHFREG
                     VPVIMNLSDMEDADAKRLVDFAAGLIFGLRGSIERVTSKVFLLSPHDVSVAAEDKERI
                     AGGFFNQS"
     gene            792..1073
                     /locus_tag="VFB89_15375"
     CDS             792..1073
                     /locus_tag="VFB89_15375"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HZM28729.1"
                     /translation="MGLIINWILWIFLLILFARMILSWVPVLVRDWEPRGPMLVVAEI
                     IYSITDPPLRLLRKVLKPVRVGNMMLDLAFIGLAILVSILMQVNRSVFF"
     gene            1201..2055
                     /locus_tag="VFB89_15380"
     CDS             1201..2055
                     /locus_tag="VFB89_15380"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HZM28730.1"
                     /translation="MTLTLDDIRNKRFRMARKSGYEVLEVDEFVDEVEESFAQLNEEN
                     QNLKKQIEALKAAPPAPQPIAPAAPTVQAPPPRQPAPPPSQSGTIVVTTGKEASAAVV
                     RLVEMSTEQAERLVEEATDDANRIREEANRTAHQLTTDARTRAERVESEARVNAERLQ
                     ADALGRAEKLDREIESRRGEMFGDLERQRDDLTASVAALRNFETAYRTNLATHLRRQI
                     ETLESGRAEPVDVPDMVRDIKPAESSSNGTANEHGGIDQPHREDAPSYSGGGSNTPRL
                     DALLGDQR"
BASE COUNT          465 a          711 c          725 g          388 t
ORIGIN      
        1 gaccccagca ccattcgccc cggctccagt gacggtgcca aggcgtaccg cgttcgacga
       61 tgatctcgac atccctgact tcctgaagtg acgcgtgtcg ggcctgatca tcaagatgcc
      121 ggacggtaat ctcggcaaca gacaacgcgc tgactgcggc gcaggtgtgt cacagattcg
      181 aggcggatga gatggcggaa cgactgaagc gcgcagcagc ttggctgggc ctcgtcaccg
      241 acgatcggta cgcagactat gacgatgtgg tcgaccttga agacttgacc gaggacagtg
      301 cagatgagcc gctcggcaca ccagacaagc ccgctacggt gaccccgctc gaaagccggc
      361 ggccggtccc ggtggcagcg cccgccgggc cgactcttgc tcctcaccca cggacggccg
      421 atatgtcccg gatcatgact gttcatcccc gcacctataa cgaagcgcgc atcgtcggcg
      481 agcacttccg cgagggtgtg ccggtcatca tgaacctgtc cgatatggag gacgccgacg
      541 cgaagcggct ggtcgacttc gccgctggtc tcatctttgg tctacgtggc agtatcgaac
      601 gcgtcaccag caaggtgttc ctgctctccc cgcacgacgt cagcgtggcc gcggaagaca
      661 aggagcggat tgcgggcggg ttcttcaacc agagctagct gaggatcccg ctggactggt
      721 ctgtgcgcct aggctggtgt cacgcctaga gtagaccgac gcaatagaga caggcctcaa
      781 ccgtggttgt cgtgggactc atcatcaact ggatcctgtg gatctttctg ctgatcctgt
      841 tcgcgcgcat gattttgtcc tgggttccgg tactcgtccg cgactgggaa ccaaggggac
      901 cgatgctggt cgtcgctgag atcatctact ccataaccga ccccccgctc cggctgctcc
      961 gcaaggtgct caagccggtc agagttggca acatgatgct cgatttggcc ttcatcggcc
     1021 tcgcgatcct ggtcagtatc ttgatgcagg tcaaccgcag cgtgttcttc tgaattcgtc
     1081 cggaacccag agctagcgac ttctttggct cggctgtacg cccccaccaa attgggtacg
     1141 gtagggccgg tgaggcgacg gcccccacca aaaagttcca cctagatacc gaggtgcacg
     1201 atgacgctga cgctcgacga catccggaac aagcggttcc ggatggcccg caagtccggc
     1261 tatgaagtgt tggaggttga cgagttcgtc gacgaggtcg aggagagctt cgcgcaactc
     1321 aacgaggaaa atcagaacct caagaagcag atcgaggcgt tgaaggcggc gccgccggct
     1381 ccacagccca ttgcgcctgc agctcccaca gtccaggccc cgccgccccg gcagcctgca
     1441 ccgccgccgt ctcaatccgg cacgattgtg gtgaccacgg gcaaagaagc cagcgccgcc
     1501 gtcgttcgcc tcgtggagat gtccaccgag caggccgagc gtttggtgga agaggccacc
     1561 gacgacgcca accggatccg tgaagaggcc aaccggactg cacaccagct caccacggat
     1621 gcccgcactc gagcggagcg agtggagtct gaggcgcggg tcaacgcaga gcggctccag
     1681 gctgatgcgc tcggacgggc ggagaagctc gatcgggaga tcgagagtcg ccgcggcgag
     1741 atgttcggcg acttggagag gcagcgggac gatctgacag catccgtcgc agcgctacgc
     1801 aacttcgaaa cggcgtaccg gaccaacctc gccacccact tgcgcaggca gatcgagaca
     1861 ctggagtccg gccgagccga gcctgtcgac gtacctgaca tggtgcgtga catcaagccg
     1921 gccgagagca gctccaacgg aactgcgaat gagcacggcg ggatcgatca acctcaccgc
     1981 gaggacgctc caagctacag cggaggtggc tccaacactc cccgtctgga cgcgctgcta
     2041 ggagaccagc gctagctgac ggcagcactg atcaagccac agggcccgct tcggcgggcc
     2101 ctgtggcgtc gtgattagcg aacgacggga tgcggcaacc tagcgaaaat ctcagcgagt
     2161 ggagcgcgcg ccgtcgtggt gaggaggagc gagccgacat gcttttggtc ggcgagcgac
     2221 gacgaactag gcggctgcct ggcgggccgc tcacatatgt gtggagagta atacatagca
     2281 cctcattgg
//
LOCUS       DASRJQ010000682         2249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_211946, whole genome shotgun sequence.
ACCESSION   DASRJQ010000682 DASRJQ010000000
VERSION     DASRJQ010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211946"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VFB89_15385"
     CDS             <1..489
                     /locus_tag="VFB89_15385"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HZM28731.1"
                     /translation="RYLGLLEASFVLNRLTPYLQSRAARLIKSPKILMTDSGLTAHLA
                     SVSDLGVTAGEPLRGALFETYVGQNLLALLGSHLPKARLHFWSIQGRYEVDFVITTGR
                     SSIGIEVKASSRFSEKELGGLQAFLASTPGARAGILAYNGTEALAVGKDLYAIPLGML
                     LS"
     gene            complement(580..1050)
                     /locus_tag="VFB89_15390"
     CDS             complement(580..1050)
                     /locus_tag="VFB89_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28732.1"
                     /translation="MRSKIVAAALILAAISMTGSAVTREEAFGVRAARIVAALEAQPE
                     LVAAAQAAAIAAMAPDQAREKAVSKVDKNSFTLIGNVFFYDTDFLSDGTPITVGVADV
                     RKPAQQDIYFSCLGSLAATCRALGRRQLEGWLLAGDGVNSDEYLHFLIVTKVKK"
     gene            1192..1602
                     /locus_tag="VFB89_15395"
     CDS             1192..1602
                     /locus_tag="VFB89_15395"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HZM28733.1"
                     /translation="MGETPAPPVVLFDGVCNLCSGSVQFLLKRDPEGRFRFASLQSEA
                     GRSLMAEHGLDVDALSSVLLIEDGQVWQESSAALRIARHLPGAWKLLRVFAAVPRPLR
                     DAAYRWIARNRYRWFGRTETCWLPTPELKARFLG"
     gene            1615..>2249
                     /locus_tag="VFB89_15400"
     CDS             1615..>2249
                     /locus_tag="VFB89_15400"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HZM28734.1"
                     /translation="MSARNLLGDPEYFDIALRLLAALVVGGLIGLERTYRGRPAGFRT
                     HALVCLSTSLLMLITVYETRWFPGITQGRISLDPTRMAQGIMTGIGFLGAGTIMKEGS
                     SVRGLTTAASLWITAAIGILVGIGFFFPALCATVLTLVTLSLFRWVEDQIPAQFYANL
                     ILRFALHAAMPEPELRRMLKEHGFTMHRLSYRLDYDSNYFEYQAIIRSNRA"
BASE COUNT          348 a          778 c          716 g          407 t
ORIGIN      
        1 cgctatctgg gcctgctcga ggcgtctttc gtgctcaacc gcctgacccc ctacctccag
       61 agccgagcgg cacggctgat caagtcgccg aagatcctga tgaccgattc cggtctgacg
      121 gcgcatctgg cgagcgtctc cgatctcggc gtgacggcgg gtgagccctt gcgcggcgcg
      181 ctgttcgaga cctatgtcgg gcagaacctc ctggccctcc tcggctctca cctgcccaag
      241 gcgcgcctgc acttctggag catccagggg cgctacgagg tggatttcgt gatcaccacc
      301 gggcggagct cgatcgggat cgaagtcaag gccagcagcc gtttcagcga aaaggagctc
      361 ggcgggctcc aggccttcct cgcctccacc cccggagcgc gggcgggtat cctcgcctac
      421 aacggcacgg aggctctggc agtcggcaaa gacctctacg cgatcccgtt gggcatgctc
      481 ctgtcctgac cccgatcaag gatccagcgg gccgtcaccg ccgagggccg cgagcgtggt
      541 ccgcttgcgg ccgaaagact gggagccgat cagcctgacc tacttcttca ccttcgtcac
      601 gatgaggaag tggaggtact cgtccgaatt gaccccgtcg ccggccagca gccagccctc
      661 gagctgacgg cggccgaggg cgcggcaggt ggcggcgagg ctgccgaggc acgagaagta
      721 gatgtcctgc tgggccggct tgcgcacgtc cgccacgccg acggtgatcg gcgtgccgtc
      781 gctcaggaag tcggtgtcat agaagaagac gttgccgatc agggtgaagc tgttcttgtc
      841 gaccttggag accgccttct cccgcgcctg atccggtgcc atggccgcga tagccgcggc
      901 ctgcgccgcg gccacgagct cgggctgtgc ctcgagcgcc gccacgatcc gcgccgcgcg
      961 cacgccgaac gcttcctccc gcgtgaccgc cgagcccgtc atcgaaatcg cggccaggat
     1021 caacgccgcc gctacgatct tgctgcgcat gattcctcct cccggcttcg gcgcggcgga
     1081 agtgccgggc cggtcgccgt tcaacgtcgt gcatctgaag atggccgctc ggcgggaaac
     1141 atttcaagca ggcatgcgca cgggagcatg tcaggtctat gatcctgcgg tatgggcgag
     1201 actccggcac cgcccgtcgt cctcttcgac ggcgtctgca acctgtgcag cggctcggtc
     1261 cagttcctcc tgaagcgcga cccggaggga cggttccgct tcgcctcgct gcagtcggag
     1321 gccgggcgaa gcctgatggc ggagcatggg ctcgacgtgg acgccctgag cagcgttctg
     1381 ttgatcgaag acggccaagt ctggcaggag agctcggcgg cgctgcgcat cgcgcgccac
     1441 ctgccgggcg cctggaagct gctgcgggtc ttcgcggccg tcccccggcc gctgcgcgac
     1501 gccgcctacc gctggatcgc ccgcaaccgc taccgctggt tcggcaggac cgagacctgc
     1561 tggctgccga ccccggagct caaggcgcgg ttcctgggct gactgcgaca gatcatgagc
     1621 gcaagaaatc ttcttggcga tcctgagtac ttcgacattg ccttgcggct gctcgccgcc
     1681 cttgtcgtcg gcggtctcat cgggctggag cggacctatc gcggccggcc cgccggcttt
     1741 cgcacccatg ccctcgtgtg cctctcgacg agcctcctga tgctcatcac cgtctacgag
     1801 acccgctggt ttcccggcat cacccaaggg cgcatctctc tcgatccgac gcggatggcg
     1861 caagggatca tgaccggcat cggttttctt ggtgcaggca cgatcatgaa ggaggggtcg
     1921 tcggtccgcg gtctcacgac cgcagcctcc ctgtggatca ccgccgcgat cggcatcctc
     1981 gtcgggatcg gattcttctt ccccgctctc tgcgccacgg tgcttacact cgtcactctt
     2041 tcgctgttcc gctgggtcga agaccagatc cccgcgcagt tctatgcgaa cctgatcctg
     2101 cgcttcgcac tccacgcggc catgccggag cccgagctgc gccggatgtt gaaagagcac
     2161 gggttcacca tgcacaggtt gagctaccgg ctcgattacg actcgaacta cttcgaatac
     2221 caggcgatca tccggagcaa ccgtgccga
//
LOCUS       DASRJQ010000683         3555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2093404, whole genome shotgun sequence.
ACCESSION   DASRJQ010000683 DASRJQ010000000
VERSION     DASRJQ010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2093404"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..1023
                     /locus_tag="VFB89_15405"
     CDS             109..1023
                     /locus_tag="VFB89_15405"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HZM28735.1"
                     /translation="MASGSILVTGAAGQLGAVGRTVTGLLLGRGLPVRAMVRRADDRA
                     AALRAAGAEVVVGDLLEPADVDRVVSGCRRVYFGMSVSAGYLEATMTMAAVAREVEVN
                     ALVNMSQMTVSQMSIQNTTPSPQQRQHWLGEQALAWSGLPVVTIRPTVFLEGFFLPLT
                     GPSVRDRGRIELPFGRGKTNPVASADVAQVVAAVLADPGPHLGRIYELTGPLSQDLHG
                     IAREYSEALNREVTYADIPPEDWERELKRVGLPQHLTHHLVTMAELNRAGRYDRMADG
                     VERVTGRPAMSVREFVSLHADEFGGRRS"
     gene            1601..1753
                     /locus_tag="VFB89_15410"
     CDS             1601..1753
                     /locus_tag="VFB89_15410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28736.1"
                     /translation="MLAIVPFHIQLVEVSLLFAIVNSQSAEADGDHAESSKAAFVSAR
                     LGGGAA"
     gene            1809..3011
                     /locus_tag="VFB89_15415"
     CDS             1809..3011
                     /locus_tag="VFB89_15415"
                     /EC_number="1.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF006941.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HZM28737.1"
                     /translation="MEVSTAYLPQEIFAPSDRGNVPPPRVFISPQRYIQGRGVLRGVG
                     RYLSLMRAKRVALLMSERGSRSEGVELLDGLRSAGIEPVLGTFRGECSIEEITAHVEA
                     LSKERIDCVIAAGGGKCIDTGKAVAFRLSTPVVIVPTLASNDAPCSALSILYSPAGIS
                     TGAEFYPNSPAFVVVDTGIIAAAPERYLVAGMGDAMATWYEARVCLLNQAAVTTVGAR
                     PTLASCAIGEVCAQTLFKEGRAAAAAVVAHTVNESLETVVEANTLLSGLGFESGGLAV
                     AHAVAQSYTAIPKVHANYLHGEMVAMGTLAQLMMESRPDEAARVAEFFATVGLPIHLG
                     QLSLGADDQSALEVVSEGTLAFPFAANMPMPVTAELVRSAILDAHRLGLAVVKKTGDA
                     AYRRLQRC"
BASE COUNT          668 a         1072 c         1134 g          681 t
ORIGIN      
        1 tttcgtatcc tctgaactca gagaacgcca ggccattcgg cactcgcacg ctgtacttgg
       61 cctggccagc gtcctgggcg tacagagctc gagaaaggtg aagctcgcat ggccagtgga
      121 tcgatcctcg taaccggggc agccggtcaa ctcggggcgg tcgggcgaac cgtgactggc
      181 ctgctcctcg gtcgcgggct tccggttcgt gccatggtcc gccgcgcgga cgaccgggcg
      241 gcggccttgc gggccgccgg cgccgaggtg gtggtcggag acctcctcga accggccgac
      301 gtcgatcggg tcgtcagcgg ctgtcggcgg gtctacttcg gcatgtccgt atccgccggg
      361 tacctggagg cgaccatgac catggcggca gtcgcccggg aagtcgaggt gaacgccctg
      421 gtcaacatgt cccagatgac cgtctctcag atgagcatcc agaacaccac cccgagccct
      481 cagcagcggc agcactggct cggcgagcag gcgctcgcgt ggtccggcct gccggtcgtc
      541 accatccggc cgacggtctt cctggagggc ttcttccttc ccctcacggg tccgagcgtc
      601 cgcgaccgag gccgcatcga actgcctttc gggcggggca agactaatcc ggttgcctcg
      661 gcggatgtcg ctcaggtcgt tgctgccgtc ctcgccgacc ctgggccgca cttaggccgg
      721 atttacgaac tcactgggcc gctgtcgcag gacctgcacg ggatcgcccg ggaatactcg
      781 gaggcgctga accgtgaggt cacgtacgcg gacattcccc ccgaggattg ggaacgggag
      841 ctgaagaggg tcgggctgcc gcagcacctg acccaccatc tggtgacgat ggccgagttg
      901 aaccgggcgg gtcggtatga ccggatggcc gacggagtgg agcgggtgac cggccggcca
      961 gcaatgagcg tccgggaatt tgtgtcgctt cacgcggatg agttcggcgg tcgccgatct
     1021 taaaggtccg gcacgccgaa caacccgctg caaccgaccg ctgagaagct cggcggctga
     1081 agcgggctcg ttagtcgtac ttcccgaggc cgcccttttt tgcgcctatc aagtcgaatg
     1141 aagaggagca gccgcaaaag cagttggcgg tatttcaaag ccgttgtcaa cggccgcaac
     1201 tttcagctca catgggccga ggctgaaaac gaaaggccca gaatcaagcg gaaaggtttt
     1261 tacacaacgg tccatgttcg agcgagaagt caaaaagaag ccgagtcacg agcgatgaag
     1321 ctgcttcgtg cagacaaggc tctccgcaaa agcgttcgaa atcccgcatc ggatcctcct
     1381 ctgatgtttc ttgaggcact tgaggagttg tcctcattca agggttgccg gctgccgcgc
     1441 acgggttttg ctttctatag tgagcgagga ccgcgcacca agcggagatc cagagaagcc
     1501 accgattgag acttacagag tatagtggcg acactctctg tcgctcgaaa cactcgatag
     1561 ccttcggttc tccccgccct aacgtgggag agcggagccg atgctcgcga ttgttccttt
     1621 tcacatccag ctcgtcgaag tgtcattatt atttgcaatc gtcaatagcc agtcggctga
     1681 agctgacggc gaccatgcag aatcctccaa agccgccttc gtctcggcgc gactgggagg
     1741 tggggcagcg tagcaggaat cgttggccat tgcgccgtcg aacttgagaa gtcaggaggg
     1801 accgattcat ggaagtatcg accgcttatc tgccgcagga aatcttcgct ccctccgacc
     1861 gggggaacgt accgccgccg cgcgttttca tctcgccgca acgttacata cagggccggg
     1921 gcgtgcttcg cggcgtgggc cgatatctct cactgatgcg ggcgaagcgt gttgcgctgc
     1981 tcatgtcgga gcgaggaagc cgcagcgagg gggtggagtt gctggacggc ctgcgctccg
     2041 ccggcatcga gcccgtcctc ggcactttta ggggcgagtg ctccatcgaa gaaatcacgg
     2101 ctcacgtcga ggcgctgtcg aaggaaagaa tcgactgcgt catcgccgcg ggcggcggga
     2161 agtgcatcga caccgggaag gccgtggcat ttcggctgag tacgccggtg gtgatcgtgc
     2221 cgacgctggc ttccaacgat gcaccctgct cggcgttgtc gatcctctac tctcccgctg
     2281 ggatttccac cggagcagag ttctacccca acagtccggc cttcgtcgtt gtcgacaccg
     2341 gcatcatcgc ggcagcgcct gaacgctacc tcgttgccgg catgggcgac gcgatggcca
     2401 cctggtacga ggcgcgcgtg tgccttctga accaggcggc ggtcacgacc gtcggcgcgc
     2461 gccccacgct cgcttcctgc gccataggcg aggtctgcgc ccagaccctg ttcaaggagg
     2521 ggcgtgcggc cgccgcggcg gtggtggcgc acacagtgaa cgaatcgctc gaaacggtcg
     2581 tcgaggcaaa tacgttgttg agcgggctcg gcttcgagag tggaggcctc gcagtcgcgc
     2641 atgccgtagc ccagagctac accgcgatcc cgaaggtgca cgcaaactat ctccacggcg
     2701 agatggtcgc catgggcact ctcgcccaac tgatgatgga atcgcgtccc gacgaggcgg
     2761 ccagagttgc cgagttcttc gcgaccgtcg gtctgccgat ccatctcggt caactctccc
     2821 tcggtgccga cgaccagagt gcgctcgagg tagtcagcga aggtacgttg gcattccctt
     2881 ttgccgccaa catgccgatg ccggtcaccg cggagttggt ccgatcggcg attctcgacg
     2941 ctcaccgtct gggactggcc gtggtcaaga agacggggga cgcagcctac agacgcctac
     3001 agcgctgctg aggctcgatt agtgtcgggg tggcaatgcc ggaagggact gcccagaggt
     3061 gccgtgccgc ccaacaacag catgcagcgg acggcgcagc gcgccgctga tgctgagcgt
     3121 cgggccgcct tcttttacgc tcggtgacaa tgcagaagag tttcagattt attctttgat
     3181 ttctcgcagc ctttccttgt tgggttaatc tccatttcac agattgtcac atatactttg
     3241 ctgacaagag cgaagacggc aggctgcact gaatgcgctt gaatctggcg aggatctcga
     3301 agagatagaa aagcgattgg tgaaggtgtt ggactgacat cggagacgaa agagatacag
     3361 ccagaagctt tcctcctgcc tcgcggcccg gatcgcctcg acttgtttgt agtgcggctg
     3421 atcgaccaca gctgcccgac cagggtcagc ggactcaatg cgccgcacgg ctctcagggg
     3481 gaagctctta gttcataccg gggccatttt gagatcagcg acggagatcc catggacaca
     3541 ccaagaaagt tcttt
//
LOCUS       DASRJQ010000684         3131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2424898, whole genome shotgun sequence.
ACCESSION   DASRJQ010000684 DASRJQ010000000
VERSION     DASRJQ010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2424898"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VFB89_15420"
     CDS             <1..366
                     /locus_tag="VFB89_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28738.1"
                     /translation="GPWAPAAPIRQAMTAEMQAAFKEYNPEPIRVPALAMYAVPKTPA
                     DLMRRWYPANDSTVRQGVEKLFPLARQRFERHAKWFETFSGRGRVTEISGAHHLFISN
                     PRDVLEQIDAFVAALPRAP"
     gene            complement(377..1189)
                     /locus_tag="VFB89_15425"
     CDS             complement(377..1189)
                     /locus_tag="VFB89_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28739.1"
                     /translation="MRSQDEGGAIRVVSTGHALFAAAMIWLGAMGLSKGEFVSVWQPV
                     PKWVPAREVLAYLCALISLGCGIGLLWRRTAAVAARVLFASLLLWLLVLRVPNFFFQT
                     PLVLVGWTFGSTAVMVAAAWVLYVWLAGDRDRQRLGFLADERGVRIARALYGLSLIPF
                     GLAHFMYLNATTVLIPDWLPGHVAWAYFTGAAFIAAGLAVTLGVRARLAAALSTLQLG
                     LFALIVWVPRVLAGTVNAFQWGEFVVTCALTAGAWVVTDSYRGTPGLAVGKR"
     gene            1396..>3131
                     /locus_tag="VFB89_15430"
     CDS             1396..>3131
                     /locus_tag="VFB89_15430"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HZM28740.1"
                     /translation="MRHAISLSMMYGLALLAKVSVVHAQRQVAAPITDPNDPRFITEQ
                     TRKQGGPMPAEQLALVFDHLDHALKVFPADKRIEGVTTLSFTTKAPIGTVILDLYPKY
                     GIAAISIDGTVVPPSRYSNPEGQLRITPPAPFPAGAKFTVQVVYSGTPPLAKRPPWEG
                     GTTWTTTPDGKDPWIDTSLWGGGCDLLYPCLDHPTLKPATTDLHYTVPTGLMAPGNGA
                     LVSRIEKDGWTTWNWHARSIHTYGSVLNVGPYKVLAGDYKSRFGNTIPMRLYYIPGEE
                     EDAADLFSEFPQTLDFWENVIGPYPWADQKMGVVRVPFSGLENQTLIGHSGRYEKTAF
                     GWNWLLNHEFAHEWFANQLSNANYDDLWLHEGLGSYAQPLLAEYLGGELDYMAQLRSQ
                     RNGIRNEQPLVTGRERSEKEVYADSGGPRGDIYPKGSWVAHTLRRLIGDDAFFTSIRT
                     LVYGRPDPRPGNFTPQFGTTQGFLQIVNRVTGKDYKWFFDVYFYQAALPQVVSIRENG
                     MLKVRWKVPGNLPFPMPLEVRVDGRIVTLPMTNGTGETPAAAQAGVTIDPSSKILRQL
                     NALDAFQAWQASR"
BASE COUNT          617 a         1021 c          984 g          509 t
ORIGIN      
        1 ggtccctggg cgccggccgc acccattcgt caggccatga cggctgagat gcaagccgcg
       61 ttcaaggagt acaaccccga gccgattcgg gtgcccgcgc tcgcgatgta cgccgttccg
      121 aagaccccgg ccgacctgat gcggcgatgg tacccggcga atgactcgac cgttcgtcag
      181 ggggtcgaga agctcttccc cctggctcgg cagcgctttg agcgtcacgc caagtggttc
      241 gagaccttta gcgggcgcgg gcgggtgacc gagatctcag gcgcccatca tcttttcatc
      301 agcaacccgc gtgatgtcct cgagcagatc gacgcattcg tggcagcgct tcccagggcg
      361 ccctgacccg ggccggctaa cgcttgccca cggcgagccc gggcgtgccg cgatatgaat
      421 ccgtcaccac ccaggcgccc gcggtcagcg cgcacgtcac cacgaactcg ccccattgga
      481 aggcgttgac ggtgcccgcc aacacccggg ggacccacac gatcagagcg aacaggccca
      541 gctgcagggt cgatagcgcg gcggccaggc gagcgcgcac accgagcgtc actgccagcc
      601 ccgccgcaat gaaggcggcg ccggtgaagt atgcccacgc cacgtgcccc ggcagccaat
      661 cgggaatcag caccgtggtc gcgttgagat acatgaagtg ggccaggccg aaggggatca
      721 gggagaggcc gtagagcgcc ctcgcgatgc gcacgcccct ctcgtcggcg aggaaaccca
      781 gccgctgccg gtcgcgatca ccggcgagcc agacgtagag cacccaggcg gccgccacca
      841 tcacggccgt cgaaccgaac gtccagccaa ccagcaccag gggagtctgg aagaagaagt
      901 tgggcactcg gaggaccagc agccacagca ggagcgaagc aaagagcacg cgggcggcga
      961 cggccgcggt gcgccgccag aggaggccga taccgcatcc aagggaaatc aaggcgcaga
     1021 gatatgccag cacttcgcgc gcaggcaccc atttcggcac cggctgccac accgagacga
     1081 attcgccttt gctgaggccc atcgctccga gccagatcat ggccgcagca aacagggcgt
     1141 gccccgtgct tacaacccgg atcgctccgc cctcgtcctg ggatcgcatg tcgttcaggg
     1201 agaacctcgg atcgagagtc aataagaaag gtggccgata cacgccgctc tcaagatagc
     1261 ctgccccatt ttttcaggag cagactcgtt ccgggtcggt ttcctgcttc agcaccggtt
     1321 gcacaccgaa tgcaccggtt tgacaggctt tcgcctggtg aggtatgctg tcgcatcgaa
     1381 acggggagaa tcaggatgcg acacgcgatc agcctgtcga tgatgtacgg cctggcactg
     1441 ctggcaaagg tgtcggtagt gcacgctcag cggcaggtgg ccgcgcccat cactgacccc
     1501 aatgacccga gattcattac cgagcagacc cggaagcagg gcggacccat gccggccgag
     1561 caactggcgc ttgtcttcga tcatctggat catgcgctca aggtgttccc ggcagacaag
     1621 cgcatcgagg gcgtgacgac actcagcttc acgaccaagg cgccgattgg taccgtgatc
     1681 ctcgatctct atcccaagta cgggattgcc gcgatctcca tcgacggcac tgtggtccca
     1741 ccatccaggt attccaatcc cgagggccag ctccgcatca caccccctgc tccgttcccg
     1801 gccggcgcga agttcacggt gcaggtcgtg tattcgggca cgcccccgct cgcaaagcgt
     1861 ccgccgtggg aaggcggaac gacctggacg accacgccgg atggcaagga tccgtggatc
     1921 gatacctcgc tgtggggcgg cggctgcgac ctgctgtacc cgtgcctcga tcacccgacc
     1981 ctgaagccgg cgaccacgga ccttcattac acggtcccga caggcttgat ggcaccgggc
     2041 aacggcgcgc tggtcagccg gatcgaaaag gatggctgga ccacgtggaa ctggcacgcg
     2101 cggagcatcc acacctatgg ctcggtgctc aatgtcggtc cgtacaaggt gctggcaggc
     2161 gactacaaga gccgcttcgg caacacgatc cccatgcgtc tctactatat ccccggcgag
     2221 gaggaggatg ccgccgactt gttctccgag tttccgcaga cgctggattt ctgggagaac
     2281 gtgattggac cgtatccctg ggcggatcag aagatgggtg tggtccgggt tccgttctca
     2341 gggctcgaga accagaccct catcggtcac agcggccgct atgagaagac ggcttttgga
     2401 tggaactggc tgctcaatca cgagtttgcc catgagtggt tcgccaacca gctctccaac
     2461 gccaactacg atgatctctg gctgcacgaa ggactcggct cctacgcgca accgctgctc
     2521 gcggagtacc tcggtggcga gctggattat atggcgcagc tgcgatcaca gcgaaacggc
     2581 attcgcaacg aacagccgct ggtgacgggg cgggagcgtt cggagaagga agtgtacgcc
     2641 gactccggcg gcccccgcgg tgacatctac cccaagggca gctgggtcgc gcacacgctg
     2701 cgcaggctga ttggtgacga cgcgttcttc acgtcgatcc gcaccctggt ctacggccgg
     2761 cccgatccgc gaccaggcaa cttcacgccg cagttcggca ccacgcaggg ctttctccag
     2821 atcgtgaatc gcgtcaccgg gaaagactac aagtggttct ttgatgtcta tttctaccag
     2881 gcggcactgc cacaagtggt cagcatacga gagaacggca tgctcaaggt ccgctggaag
     2941 gttcccggca atctgccgtt cccgatgccg ctcgaagtgc gggtcgatgg caggatcgtt
     3001 accctgccga tgacgaacgg caccggcgag acgccggcgg cggcgcaagc gggggtcacg
     3061 atcgatccat cgtcgaagat tctccggcag ctgaatgccc tcgatgcgtt ccaggcctgg
     3121 caggcgtcgc g
//
LOCUS       DASRJQ010000685         3022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3509064, whole genome shotgun sequence.
ACCESSION   DASRJQ010000685 DASRJQ010000000
VERSION     DASRJQ010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3509064"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VFB89_15435"
     CDS             complement(<1..672)
                     /locus_tag="VFB89_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28741.1"
                     /translation="MRHRFGACPVPLAALVLAVILPAAARAQDSNYWSLAYGTRSQLL
                     GGVMIGSPGDISSVYYNPGALALAQNVELLISGSAYRYQRLSIDGGSGPGRNLVSSWL
                     AATPSLFAGEIPILDHDRLAYSFLGRHQSNMEMMGRFTAGSEIPPTSPDLSALELDFN
                     QNLSENWFGLTWAHKLSSTLGIGVSPYLGVRSQNTGISAFRSSQDAASTASVFNVSRR
                     LDYLHW"
     gene            complement(732..977)
                     /locus_tag="VFB89_15440"
     CDS             complement(732..977)
                     /locus_tag="VFB89_15440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28742.1"
                     /translation="MKLTLILLFVLSVLVGVAVGEWFFRLYMKALPPMAHSDFNTSAS
                     RIAHLMYGAGLGLVLFLWSLLGMAAANILRKKPASGA"
     gene            complement(1191..1844)
                     /locus_tag="VFB89_15445"
     CDS             complement(1191..1844)
                     /locus_tag="VFB89_15445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28743.1"
                     /translation="MAVAGAIAAVCGFVAGPMPLAIIGLVLATAGSWNFWRPSITGLV
                     VDGVTMIMTGALIALAWLWIPDARESSAGKWILAGGLQVAWGIRRLALYATARHAYND
                     PDAIARLGSIVRDLSRGRAKDDASIAEFRTGRFRARNRLGLYAEGAIGLLEHQALRLE
                     KRTDIWIEPQGTTSLGRSIKVRVQMSDLQLAGVMQVAHFERFERWKLGQSQPRSIAA"
     gene            complement(2047..2562)
                     /locus_tag="VFB89_15450"
     CDS             complement(2047..2562)
                     /locus_tag="VFB89_15450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28744.1"
                     /translation="MRFKAVLFSSLILMIAFTVPVVPAQAQTASEIDSYIELLRSDLK
                     TGKKELITALLDLPADKAPGFWEIYNKYQLDMDKVSDQRVANIKDFGANFEKMTEAKA
                     AELMKSAVDFQTKRIGVHKSYMGKFQKAIGAVETAKLMQLESVIQDLVDLQIHANLPL
                     IEKTAVETSGK"
     gene            complement(2748..>3022)
                     /locus_tag="VFB89_15455"
     CDS             complement(2748..>3022)
                     /locus_tag="VFB89_15455"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28745.1"
                     /translation="VRIEGLLREIVNAHAEVLKEPAPVIRLNQLADSSLSFVVRVWVL
                     KDRYWQVYWDITRAVKTRFDQEGIKIPLPQREVYMNMIPGSVARPA"
BASE COUNT          550 a          959 c          996 g          517 t
ORIGIN      
        1 ccagtgcaga tagtcgagcc tccggctcac gttgaagacc gaggcggtgc tggcggcatc
       61 ctgcgacgag cggaacgccg agatgccggt attctgactg cgcaccccca ggtacggcga
      121 cacaccgatt cccagggtgg acgacaactt gtgcgcccac gtcagtccga accagttctc
      181 actcaggttc tggttgaagt ctagctcgag cgccgacaga tccggggacg tgggcgggat
      241 ctccgagccg gcggtgaatc gccccatcat ctccatgttc gactggtggc gaccgagaaa
      301 cgaataggcc agtcgatcgt ggtcgaggat cgggatctca ccggcaaaca gggagggcgt
      361 cgccgcgagc caggaggaga ccagattcct cccgggaccg ctgcccccgt cgatcgagag
      421 gcgctggtac cggtaagcgc tgccggagat cagcagttcg acgttctgcg ccagcgcgag
      481 cgccccgggg ttgtagtaga cggagctgat gtcccccgga ctgccgatca ttacgccgcc
      541 gagcagctgc gagcgcgtgc cataggccag agaccagtag ttgctgtcct gcgcccgggc
      601 agctgccggg aggatgacgg cgaggaccag cgcggcgaga gggacggggc acgcaccgaa
      661 tcgatgccgc atggctcctc cggttcattc gaggcattca cacgtccggc ggtgtgccgc
      721 cgaacaatcc gtcaagcgcc cgaggcgggt ttcttgcgca ggatgttcgc ggccgccatg
      781 ccgagcaatg accacaggaa caggacgaga cccagccccg cgccgtacat caagtgtgcg
      841 atacgcgaag cgctggtgtt gaaatcgcta tgggccatgg gcgggagcgc cttcatgtag
      901 aggcgaaaga accactctcc caccgccacg ccgaccagca cgctgagcac gaagaggagg
      961 atgagcgtga gcttcatggc actcaacgcc gtgctccgtt ctggccggga tgcgggcctt
     1021 cgagagacac gatacggatg cgcattaggc ctcccccgtg tgggctcgcg cgcgagcccc
     1081 cgcgtacgcc gtaatcaccg atcggaatgc gcaagataag gccgctgtcc cggcaaggca
     1141 accgggtaca gccgtggaat cgaaggccgg ggatctggcg cggccgtggc ctaggcggcg
     1201 atcgaacgcg gctgcgactg cccgagcttc caacgctcga agcgctcgaa gtgggcgact
     1261 tgcatgacgc ccgcgagctg caggtcgctc atctgcaccc tgaccttgat cgaccggccg
     1321 agcgaagtcg tgccttgggg ttcgatccag atatcggtgc gtttctcgag cctcagcgcc
     1381 tggtgctcga gcagtccgat cgccccctcg gcgtagagcc cgaggcgatt gcgagcgcga
     1441 aagcgaccgg tgcgaaactc ggcgatgctc gcgtcgtcct tggccctgcc cctggagaga
     1501 tcccgcacga tcgatcccag gcgcgcgatc gcgtcgggat cgttgtaggc gtggcgcgcg
     1561 gtcgcgtaga gggcgagccg gcggatgccc cacgcgacct gcagcccgcc ggcgaggatc
     1621 cacttgccgg cgctcgactc gcgtgcgtcg gggatccaca gccaggccag ggcgatgagc
     1681 gctccggtca tgatcatcgt gaccccgtca acgaccaggc cggtaatgga ggggcgccag
     1741 aagttccagc tgccggccgt ggcgagcacc aagccgatga tcgccagagg catagggccg
     1801 gccacgaacc cgcagacggc cgcgatcgcg ccggcgaccg ccatgaaggc tgcgtgccat
     1861 cgcaccgagc gcgacatgct gaggcggaaa gccgacgcgt cgaggcggtt ccaatcggag
     1921 aggtcgaaca gcggaagctc cagtggcggc aggcccgggg cgggctccta agtcatcgtc
     1981 accccgggcc tgcaagcttg agcccttctc gccgcgcaag ctcggcggcc gccactcact
     2041 tcagggctac ttcccggaag tctccactgc ggtcttctcg atgaggggaa ggttggcgtg
     2101 aatctggaga tcgacgagat cctgaatcac gctctcgagc tgcatgagct tggccgtctc
     2161 cactgcgccg atcgccttct ggaacttgcc catatagctc ttgtggacgc cgattcgctt
     2221 ggtctggaag tccacggcgc tcttcatgag ctccgcggct ttggcttcgg tcatcttctc
     2281 gaaattggct ccgaagtcct tgatgttcgc gacgcgctga tcggagacct tgtccatgtc
     2341 gagctggtac ttgttgtaga tctcccagaa gccgggtgcc ttgtccgcgg gcagatcgag
     2401 cagggcggtg atgagctcct tcttcccggt cttgaggtcg gagcgcagca attcgatgta
     2461 ggagtcgatt tcgctggcgg tctgggcctg ggcaggcacc accggaaccg tgaacgcgat
     2521 catgagtatc agcgaagaaa acagcaccgc cttaaacctc atcagacgta ctcccgtggg
     2581 gcgggctgac cacgatccgc gcgtggcccc aggtcgcgcg ttcgggtgaa tgatcgtctg
     2641 cggctcagac ggccgaaacc actcgcaggc taagtccctg cccggccgcg agtcaatgtg
     2701 ggtttggcag gtcgcattgc gcgcgtaagt ccgcgatggc cgcggcgcta tgccggccgt
     2761 gcgacggatc ccggaatcat gttcatgtaa acttcgcgct gaggcagtgg aatcttgatt
     2821 ccctcctgat cgaatcgcgt cttcaccgcg cgcgtaatgt cccagtagac ctgccagtag
     2881 cgatccttga gcacccagac gcgcaccacg aagctcagcg aggagtccgc gagctgattc
     2941 aggcggatga ccggcgcagg ttccttcagg acttcggcgt gcgcgttcac gatttctcgc
     3001 agcagacctt cgatccgcac ca
//
LOCUS       DASRJQ010000686         7273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2949547, whole genome shotgun sequence.
ACCESSION   DASRJQ010000686 DASRJQ010000000
VERSION     DASRJQ010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7273
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2949547"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VFB89_15460"
     CDS             complement(<1..292)
                     /locus_tag="VFB89_15460"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HZM28746.1"
                     /translation="MSEADSLAAVVFRIGDLVCAAPAARVREVLPRLPATRIPGVPFA
                     VEGLVNVRGGLLTVTDGHALLGRAARPDDEGAILVLDIAGRRFGLGVGQVLDF"
     gene            complement(285..1301)
                     /locus_tag="VFB89_15465"
     CDS             complement(285..1301)
                     /locus_tag="VFB89_15465"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HZM28747.1"
                     /translation="MPLTGFSLEFCVCAESTERLDRFLADQLQLSRTQAARLIADKAV
                     LVSGVVARASRLLARGDRVSVAIPSAQPPRQLRPANIPLTIVWEDEHLAVIDKPAGLV
                     VHPAPGHWDDTLVNALVARGTALGGGVQGRPGIVHRLDRDTSGLMLVAKTDLAHRRLS
                     HLLGARRIVRAYAALAWGHFSDSPTVLEAPLARHPRDRKRMAISSTGRPARTDAYVIA
                     RFDSVDLLRLELHSGRTHQIRVHLEHAGHPIVGDPVYGGGGSRRISGAARASADALER
                     AAGRQALHAAWLAFRHPLTGAALEFRSEWPPDLRAALAIAAGEELVARSDPLTYLLFF
                     NRHE"
     gene            complement(1383..1892)
                     /gene="lspA"
                     /locus_tag="VFB89_15470"
     CDS             complement(1383..1892)
                     /gene="lspA"
                     /locus_tag="VFB89_15470"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HZM28748.1"
                     /translation="MENAADRRIFWGAALLVVTLDVLTKLIAEATLLLTPGVPVVGEW
                     FQLRLVYNRGAAFGLHVGPYSRWVFFAVAVLAVFVLNRMSLHTSPGDRFRQLALGLVA
                     GGAAGNLVDRVRSSRGVVDFLDVGVGSLRWPTFNVADIAVSCGGIALAIALWREDHRR
                     GRAESAPAA"
     gene            complement(1876..2259)
                     /locus_tag="VFB89_15475"
     CDS             complement(1876..2259)
                     /locus_tag="VFB89_15475"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HZM28749.1"
                     /translation="MNKKQLGHLEKRLLEERARVMKELGYYDESFNASLQSSDGDLSS
                     YSFHMADQGTDAMEREKQFLFASQEGRYLWHVNEALRRLYKTPEKFGHCHSCGAEITF
                     ERLDALPHARLCITCKEKEEDGKRR"
     gene            complement(2282..5506)
                     /gene="ileS"
                     /locus_tag="VFB89_15480"
     CDS             complement(2282..5506)
                     /gene="ileS"
                     /locus_tag="VFB89_15480"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HZM28750.1"
                     /translation="MTFAAWPDLTPDQLEQEQLEIWKGERLFQRTVEAARSGPPYVFY
                     EGPPTANGRPGIHHVFSRTIKDLVCRFHAMQGKSVTRIAGWDTHGLPVEIEVEKELGI
                     NGKKDIERFGVADFNARARKSVFKYQSEWENLSDRIGYWLDYEHPYITCSNEYIESVW
                     WLLHRLHERGLLYRGHRVLPYCPRCGTVLSSHELALGYDEVTTNSVYVTFPLEDDPTR
                     QLLIWTTTPWTLLSNVAVAVNPELEYAEYPLDGRTIILATARADLPSGLEEIAPGAGA
                     VGTTRKSARARKQLVPFRSLTPIRTFKGRDLVGVRFQRPLDVVPLPEDRVSRVVVAGD
                     FVTADDGSGLVHMAPAFGADDYAMGQEHGLALVRPVAADGTFTGTSWPEIEGKLVTAR
                     ETNDLIIQRLKQEGRWHLTQPYTHTYPHCWRCSSPLIYYARDSWFVQTSAVKDRMLEL
                     NRRVKWHPPEVGAGRFGEWLANNVDWALSRDRYWGTPLPVWVCQGDSSHVEVISDYAR
                     LAERWGKPLPENFDPHKPFIDDYVWACHCGGLMRRTPEVIDAWFDSGSMPYAQWHYPF
                     ENEREFKTHFPADFICEGVDQTRGWFYSLLAIATTAFDDLAYKHVIVNELVLDAEGQK
                     MSKSRGNVVDPWEMIREFGADTIRVYLLASSQVWLPKRFDRRTIPEVAGGFFNTLRNT
                     YGFFSLYAGDWRAQAAPPLSRRNQLDRWLLSRLDATVEAVTAHWSGYDVTAGTRAIMD
                     FVVDDLSNWYVRLSRSRFWAPDREADPAAVSTLHEALSTVARLLAPAAPFASDWLHRA
                     LAGTSVHLQRFPVPRGQREAGLEAAMDAVRRLASLARSVRNEASLPVRQPLRRMYVAV
                     PATVRGEAFDRLMELLRLEVNVKSIEVVSSDAELVRLKAKPNFRSLGKRYGKRTPEVA
                     AAVSDLSQEQLRTTEAGRSLTLALTDGTTVSIEPEDVTVEREVASQWLVKSEGPCVVA
                     LDPELDDALRGEGFAREVVNRVQRLRKEAGYEYTTRIQLWIDGGAAALEALRPHGGFI
                     QEETLARSLKLGARASGPDGEHRLEMDGLGVTLGVRRYEERSAV"
     gene            complement(5510..6340)
                     /locus_tag="VFB89_15485"
     CDS             complement(5510..6340)
                     /locus_tag="VFB89_15485"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HZM28751.1"
                     /translation="MTAAAMLYDDIQRAAEAVRAQATLRPDIGVILGTGLGGLAGEIA
                     VDATVPYQAIPGFPLSTVESHAGRLLLGDLGGKPIVAMQGRFHRYEGYTLAQVTFPVR
                     VLHALGAKTLIVSNACGGMHPLWAPGDLVLLADHVNLLGDNPLVGANDERLGPRFPDM
                     SAPYHPALRDLARRAALELGITLREGTYVAVPGPNLETRAEYRMLRTIGADVVGMSTV
                     PEVIVANHEGMRVLGISIITDQCLPDALEPADITRIIATAVRAEPSLTRLIRRVVEMM
                     "
     gene            complement(6337..6873)
                     /locus_tag="VFB89_15490"
     CDS             complement(6337..6873)
                     /locus_tag="VFB89_15490"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HZM28752.1"
                     /translation="MSDDAFQLTPQDVRTQDFARVLRGYDPAQVDEFKHRLAEELDRL
                     ARAKVQAEEKLKSAQDQLRTHRERERHVHDALVAAQQLRSDLRVQAEQEAAVIVREAH
                     GQAAKIVDRAKLDEQLVRDRTEDAVRQFNAYVATFRLLLERQLGEVEGLQAAAREGTG
                     APRLPAVSEVEVGAGETG"
     gene            complement(6870..>7273)
                     /locus_tag="VFB89_15495"
     CDS             complement(6870..>7273)
                     /locus_tag="VFB89_15495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HZM28753.1"
                     /translation="FALIHSVDRLDLARELDRRVAENERQMILVQVNCSNEAQKGGIE
                     PDGLPALLDELAGLARLEVRGLMTMAAFTDDVAEQRRSFRLLRSLRDRAAAAGHSLPE
                     LSMGMSADYPMAVEEGATMVRLGTVLFGERS"
BASE COUNT         1287 a         2476 c         2319 g         1191 t
ORIGIN      
        1 cgaaatcgag cacctgaccc acccccagcc cgaaacgacg tcccgcgatg tcgaggacga
       61 ggatggcgcc ctcgtcgtcg ggcctggctg cccggccgag cagcgcatgt ccatcggtga
      121 ccgtgagcag cccgccgcgg acgttaacca gcccctccac ggcaaatggc acgccgggaa
      181 tccgggtggc cggcaggcgg gggagaactt cccggacccg ggcggccggc gcggcacaaa
      241 ccaggtcgcc gattcggaag accacggccg cgagcgagtc ggcttcactc atgtctattg
      301 aagaaaagaa gataggtaag ggggtcggag cgggcaacga gttcttcgcc ggcggcgatg
      361 gcgagcgccg cccgtagatc gggcggccat tcggatcgga actcgagcgc ggctcccgtc
      421 aaggggtggc gaaacgcaag ccaggcggcg tggagcgcct gccggccggc agcccgctcg
      481 agcgcatcgg ctgaagcccg ggcggccccc gagatgcggc ggctgccccc tcccccataa
      541 accggatcac ccacgatcgg atggcccgcg tgctcgaggt ggaccctgat ctgatgggtg
      601 cggccgctgt gcagctcgag ccgcaacaga tcgacactgt cgaatcgggc gatcacgtag
      661 gcgtcggttc gcgcaggcct gccagtcgac gagatcgcca tccgcttgcg gtctcgcgga
      721 tgcctggcca gcggcgcttc gagcaccgtg gggctgtccg aaaaatggcc ccacgcgagc
      781 gcagcgtagg cccgcacgat ccgacgtgct cccaacagat gactcaggcg acggtgcgcg
      841 agatcggttt tggcgaccag catgaggccc gaggtgtcgc gatcgagccg gtgtacgatc
      901 cccggccggc cctggactcc gccacccagc gcggtgccgc gggcaaccag cgcattcacc
      961 agcgtgtcgt cccagtggcc gggagcagga tgcacgacga gcccggcggg cttatcgatc
     1021 acggcgaggt gctcgtcttc ccagacgatc gtgagaggga tgttcgcggg tcgaagctgt
     1081 cgcggtggct gcgccgaggg aatggcgacg gatacgcgat caccgcgcgc gaggagccgc
     1141 gaggcgcgag ctacaacgcc actcaccagc accgctttgt cggcgatcag cctggcggcc
     1201 tgggtgcgag agagttggag ctggtcggcg aggaagcggt cgaggcgttc ggtggattcg
     1261 gcacagacgc agaattcgag cgagaagcca gtgagcggca agtcgggggg tgaggacgct
     1321 ggccaagccc cgccaacccc agcgtcctca ccccccggct tgccgctcac tggcttggga
     1381 gattatgccg caggtgccga ctcggcccgg ccccgccggt gatcctctcg ccacagtgcg
     1441 atggcgagcg cgatgccacc gcagctcacg gcaatgtccg ccacgttgaa cgtgggccag
     1501 cgcagcgagc cgacgcccac gtcgagaaag tccaccaccc cgcgcgagct gcggactcga
     1561 tcgaccaggt tgccggccgc gcccccggca acgagaccga gcgccagctg ccggaaccgg
     1621 tcaccgggcg aggtgtggag cgacatgcgg ttcagaacga agacagcgag tacggcgacc
     1681 gcaaagaaga cccatcggga atagggtccg acgtggagcc cgaacgccgc gccgcgattg
     1741 tataccagtc gcagctgaaa ccactcgccg accacgggga cacccggggt cagcagcaga
     1801 gtggcctcgg cgatgagctt ggtcagcacg tcgagtgtga cgaccaaaag cgccgctccc
     1861 cagaagatcc ggcgatcagc ggcgttttcc atcctcttcc ttctccttgc aggtaatgca
     1921 gagacgggcg tgcggcagcg cgtcgagccg ctcgaaggtg atctctgcgc cgcagctgtg
     1981 gcaatggccg aacttctccg gtgtcttgta gagtcgccgg agcgcctcgt tcacgtgcca
     2041 cagatagcga ccttcctggg acgcgaagag gaattgcttc tcgcgctcca tggcgtcggt
     2101 cccctggtcg gccatgtgga aggaatacga cgagaggtcg ccgtccgagg actggagcga
     2161 cgcgttgaat gactcgtcgt agtagccgag ttccttcatg acccgggcgc gctcttcgag
     2221 cagccgcttc tccagatggc ccagttgctt cttgttcatc tgacatccat tccgtttagg
     2281 gtcagaccgc cgaccgttct tcgtatcgcc ttacgcccaa tgtcacaccc agcccgtcca
     2341 tctccagccg atgctcgccg tcaggccccg aggcccgggc gccgagtttg agtgaacgag
     2401 caagcgtctc ttcctgaatg aatccaccgt gcgggcgaag cgcctcgagc gccgcagccc
     2461 ctccgtcgat ccagagctgg atccgcgtgg tgtactcgta ccccgcctcc ttgcggagcc
     2521 gctgcacccg gttcactacc tcgcgggcga acccctcgcc ccgcaaggcg tcgtccagct
     2581 cgggatccag cgccaccacg cagggccctt cgctcttcac cagccactgg ctcgcgactt
     2641 cgcgctcgac ggtgacgtcc tccggctcga tcgacaccgt agttccatcg gtgagcgcga
     2701 gagtgagcga ccgaccagct tcggtcgtcc gcagctgctc ctgcgataga tcggacacgg
     2761 ccgccgccac ttccggcgtg cgcttgccgt agcgcttgcc cagtgaccgg aagttcggct
     2821 tggccttgag ccgcaccagc tccgcatcgg agctcaccac ctcgatcgac ttgacgttga
     2881 cctcgagccg cagcagctcc atgagccgat cgaatgcctc accgcgaacc gtggcaggca
     2941 ccgccacata catgcggcgc agcggctggc gcactggaag gcttgcttcg ttccgcaccg
     3001 agcgcgccag tgacgcaagc cggcgcacag catccatcgc tgcctcgagc ccggcctccc
     3061 gctgtcctcg ggggacggga aaccgctgga ggtgcaccga agttccggcc agcgcgcggt
     3121 gcagccagtc gctcgcgaac ggggctgccg gcgccagcag tcgagcgacc gtcgagagcg
     3181 cctcgtgcag cgtggacacc gcagcaggat cggcctcgcg gtcgggcgcc cagaatcgcg
     3241 agcgggagag ccgaacatac cagttggaca gatcgtccac tacgaagtcc atgatcgcgc
     3301 gggttccagc cgtgacgtcg tagccgctcc agtgcgcggt gactgcctct actgttgcgt
     3361 cgagccgact cagcagccag cgatccagct ggttccttcg cgacaggggc ggcgcggctt
     3421 gggcacgcca atcgcccgcg tacaaagaga agaacccgta ggtgttccgc agggtgttga
     3481 agaaccctcc cgcgacctcg gggatggtgc ggcgatcgaa ccgcttgggc agccagacct
     3541 ggctcgacgc gagcagataa acccggatgg tgtcggcgcc gaattcgcgg atcatctccc
     3601 acggatctac cacgttgccc cggctcttgg acatcttctg gccttcggca tcgagcacca
     3661 gctcgttcac gatcacgtgc ttatacgcga gatcgtcgaa cgcagtggtc gcgatcgcga
     3721 gcaggctgta gaaccagccg cgggtctggt ccacgccctc gcagatgaaa tcggccggga
     3781 aatgggtctt gaactcgcgt tcgttctcga acgggtagtg ccactgcgcg tagggcatcg
     3841 accccgagtc gaaccaggcg tcgataacct cgggggtgcg gcgcatcagc ccgccgcaat
     3901 gacaggccca gacgtagtcg tcgatgaacg gcttatgagg atcgaagttc tcgggcagcg
     3961 gcttgcccca gcgctcggcc agccgggcgt agtctgagat cacctcgacg tgcgagctat
     4021 cgccctggca gacccagacc ggcaaggggg tcccccagta gcggtcacgg gacagcgccc
     4081 agtccacatt gttcgccagc cactccccga agcgtccggc gccaacctcc ggcgggtgcc
     4141 acttcacccg ccgattcaac tccagcatgc ggtctttcac cgccgatgtc tgcacgaacc
     4201 aggaatcgcg ggcgtagtag atgagcggac tgctgcagcg ccagcagtgc ggataggtat
     4261 gcgtgtacgg ctgcgtcaga tgccagcgac cctcctgctt aagccgctgg atgatgagat
     4321 cattggtttc acgcgcggtc acgagcttgc cctcgatttc cggccagctc gtgccggtga
     4381 acgtgccgtc agccgccacc ggacgtacca gcgccagccc gtgctcctgc cccatggcgt
     4441 agtcgtcggc gccgaaggcc ggtgccatgt gcaccagccc cgagccgtca tccgcagtca
     4501 cgaaatcacc tgctaccacg acccgcgaga cccgatcctc tgggagtggc accacgtcga
     4561 gtggccgctg gaaccgcacg cccaccagat cgcgcccctt gaatgttcga ataggagtga
     4621 gggagcggaa cggcaccagc tgcttccggg cgcgggcgct cttccgggtc gtgcccactg
     4681 ctccggcgcc gggcgcgatc tcctcgagcc cactgggaag gtcggcgcgc gctgtcgcca
     4741 aaatgatcgt ccgaccgtcg agcggatact cggcgtactc gagctccggg ttgaccgcta
     4801 ccgccacgtt cgaaagcagg gtccacggcg tggtagtcca gatcagcagc tgacgagtgg
     4861 gatcgtcttc cagcgggaag gtcacgtaca ccgagttggt ggtcacctcg tcgtagccca
     4921 gcgccagctc gtgactcgac aagacggtgc cgcagcgcgg gcaatatggc agaacccgat
     4981 gaccgcgata cagcaggcca cgctcgtgga gccggtggag gagccaccag acactctcga
     5041 tgtactcgtt gctgcacgtg atgtacggat gctcgtaatc cagccagtac ccgatgcgat
     5101 ccgagaggtt ttcccactcg gactgatact tgaagacgct cttccgagcc cgggcattga
     5161 agtcggcgac accgaatcgc tcgatgtcct tcttcccgtt gatgcccagc tccttctcga
     5221 cctcgatctc caccggaagc ccgtgagtgt cccagcccgc gatccgggtg accgacttcc
     5281 cctgcatcgc gtggaatcgg cacaccagat ccttgatggt ccgagagaag acgtgatgaa
     5341 tcccggggcg cccgttagcg gtgggcggac cctcgtagaa cacgtagggt gggccgctcc
     5401 gcgccgcttc cacggtccgc tgaaacagcc gctccccttt ccagatctcg agctgttcct
     5461 gctcgagctg gtcgggagtg agatcgggcc aggctgcgaa cgtcattcgc tacatcattt
     5521 ccaccacgcg ccggatcagt cgcgtcagcg acggctccgc gcgaacggcg gttgcaatga
     5581 tacgggtaat gtcggccggc tcgagcgcgt cggggagaca ttgatcggtg atgatcgaga
     5641 tgccaagaac ccgcatgccc tcgtgattgg cgacgatgac ctcggggacc gtggacattc
     5701 cgaccacgtc cgcgccgatc gtgcgaagca ttcggtactc cgcccgcgtc tccaggttgg
     5761 gcccaggcac tgcgacatag gttccctccc tcagcgtgat gccgagttcg agagccgcgc
     5821 gccgcgcaag atcgcggagc gccgggtggt agggcgccga catgtcagga aagcgaggcc
     5881 cgagtcgctc gtcgttcgct cctaccagcg gattgtcacc cagcaggttg acgtggtcgg
     5941 ccagcagaac cagatccccc ggcgcccaca atgggtgcat gccgccgcac gcgttggaca
     6001 cgatcagcgt ctttgccccg agcgcgtgga ggactcgaac gggaaaagtg acctgcgcga
     6061 gagtgtatcc ctcgtagcgg tggaagcggc cctgcatcgc gacgattggc ttgccgccca
     6121 aatcacccag caagagccgg ccggcatggg actctaccgt ggaaagcggg aagcccggaa
     6181 tcgcctggta aggaaccgtt gcgtcgaccg cgatctcgcc cgccagtcca ccgagtcccg
     6241 tacccaggat gacgccaata tccggccgca gcgtcgcctg cgcccggacc gcctcagccg
     6301 cgcgttggat atcgtcgtag agcattgcag cagccgtcaa cccgtctctc ccgcccccac
     6361 ttcgacttcg ctcaccgccg gcagccgcgg agcgccggtg ccttcccggg ctgcggcctg
     6421 caatccttcg acctcaccca actgccgctc cagcagaagc cggaacgttg ccacatacgc
     6481 attgaactgc cggacggcgt cttcggtccg gtctcgtacc aactgttcgt ccagcttggc
     6541 ccggtccacg atcttggcgg cctggccatg cgcctcacgc acgatgacgg ctgcctcctg
     6601 ctccgcctgg acccgcaggt cggagcgaag ctgctgcgcc gcgaccagcg cgtcgtgcac
     6661 gtgacgttcc ctttcccggt gggttcgcag ctggtcctgc gcgctcttca gcttttcttc
     6721 cgcctgcacc ttggcccggg ccagacggtc gagctcctcg gccaaacggt gcttgaactc
     6781 gtccacttgc gccggatcgt agccacggag gacccgggcg aaatcctggg ttcgcacatc
     6841 ctgcggagtc aactggaagg cgtcgtcgct catgagcgct ctccaaagag gaccgtgccc
     6901 agccggacca tcgtagctcc ttcctcaacg gccatcggat aatcggccga catgcccatc
     6961 gaaagctccg gcagagaatg cccggctgcc gctgccctgt ctcgcagcga tcgaagcagc
     7021 cggaacgatc gtcgctgctc ggcgacgtcg tcggtgaatg cggccatagt catgagaccg
     7081 cggacttcga gccgagcaag ccccgccaat tcatcgagca gcgcgggcaa accatccggc
     7141 tctatgccgc ccttctgcgc ctcgttcgag cagttcacct gaacgaggat catctgccgc
     7201 tcgttttcgg cgacccgacg gtccagttct cgggccaggt ccagccgatc gaccgaatgg
     7261 atgagggcaa agc
//
LOCUS       DASRJQ010000687         2276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2950305, whole genome shotgun sequence.
ACCESSION   DASRJQ010000687 DASRJQ010000000
VERSION     DASRJQ010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2950305"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..412
                     /locus_tag="VFB89_15500"
                     /pseudo
     CDS             112..412
                     /locus_tag="VFB89_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611786.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(552..1529)
                     /locus_tag="VFB89_15505"
     CDS             complement(552..1529)
                     /locus_tag="VFB89_15505"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HZM28754.1"
                     /translation="MTPPPPLPSWFGPRASTDDLAVAALLDQVAPARTWQDIGGEFNL
                     NVRLDGAATLVLRVHRPWALRGRVAGLRRLRERLQRAGIRVAQPAPILGRDLARVGGR
                     WAELEEFVPHVQPNPSQESYLRLFEELGRLHAGLRATWEPDLPQPLDDHRTSRQLHYW
                     LGFTRRRLGAGAEPVVRRAKLLLDELGRARRGVELPLAPIHGDYKLGNAVQLSDGSWA
                     NLDLDFVRIRERLYDVAGSLYWASESGADVDLRELCDAYDSSAPEPVTPDERRFLPAM
                     ITLIPLHWVATAGLMDERIGVHGTVGLPEAERAMDAAEAWWARREELTW"
BASE COUNT          418 a          752 c          713 g          393 t
ORIGIN      
        1 tgcgcgttat ggccgatgaa cgatacgctg gcacatccta ccgttgtaga acgtgaggat
       61 ggcacgacgg attcgcctcc aagcccatct cacaactgag gaactggcaa gtcgttatcg
      121 aggcgccagc cggagggcgc cgtggtcgtt cattccgacc gcgggggcca atttcgagcg
      181 aggtccttcg tggcggtgct caaggcgaac aacctcaccg ggtctcgtcg gccggcgaca
      241 acgccgccat ggagtcctct actcgctgct ccagaagaac gtcctggacc gccagcgctg
      301 gaccacgaga gccaagctcg cctacgcgat cctcgtgtgg atcgagcaca cctacaaccg
      361 ccggcgacgc cagcgatccc tcggcaagct cacccccgtc gagttcgagc tggcattcac
      421 cgcagtcacc gagaaacacc gaaaccggcg ggtacacggt catgtccgcg ggtcccttgc
      481 ccttgtcgct tctcccccga gtcaagggag cgaacgccac cggctccgta gagcacctct
      541 gcccagccgg actaccacgt caattcctcg cggcgtgccc accacgcctc ggccgcgtcc
      601 atcgctctct ccgcctcggg caagcccact gttccatgta ctccgattcg ctcatccatc
      661 aacccggccg ttgcgaccca gtgcagggga atcagggtga tcatcgccgg gaggaaccgg
      721 cgctcgtcgg gcgtcacggg ttcgggagcg gaactgtcgt aggcgtcgca gagttcgcgg
      781 aggtcgacgt cggcgccact ctcagatgcc cagtacaggc tgccggccac gtcgtagagc
      841 cgctcccgga tgcgcacgaa atccagatcg agattcgccc aggagccatc cgacagttgg
      901 accgcgttgc cgagcttgta gtcgccgtgg atcggagcga gcgggagctc gacgccgcgc
      961 cgggctctgc ccagctcgtc gaggaggagc ttggcgcggc gcaccacggg ctccgccccg
     1021 gcgcccagac ggcgacgcgt gaagccgagc cagtagtgca actggcggct ggttcggtgg
     1081 tcgtcgagcg gctgaggcag gtccggctcc caggtcgcgc gcagccccgc gtgcaggcgg
     1141 ccgagctcct cgaacaaccg caggtacgac tcctggcttg ggtttggctg cacatgcggg
     1201 acaaattctt cgagttccgc ccagcggcca ccgaccctgg cgaggtcccg gccgaggatc
     1261 ggcgccggct gggcaacccg aattcctgcg cgctggagtc gctcgcgcaa ccggcgcaat
     1321 cccgccacgc ggccgcgcaa cgcccatggt cgatgcacac gcaggacgag cgttgcagcc
     1381 ccgtcgagcc ggacgttgag gttgaactcg ccgccgatgt cttgccacgt cctggcaggc
     1441 gcgacctgat cgagcagcgc ggcgaccgcg aggtcatcgg tgctggccct tggcccgaac
     1501 cagctgggca gtggtggagg tggtgtcaaa gcttgggtcc ttcatcgcgg agcttcgctg
     1561 gaagcatccc atccgccggt agaaacagca tgcccggcac attgatgttc tgcgtctcag
     1621 cgttcaagaa tcggcagcct cggattctcg cacggcctcc gctgcggagt cgggtggcga
     1681 taggtggacg cggccacgcc ccgcacccag acgcgatgga agttgtccgc gcgaaggtgc
     1741 ccgatcagtc acaaccggtg tcaaccgaac ctgcagcaga cccactttgc ggattaagtg
     1801 tcgtgcccgt tgtgtgcccg atcgaacggt aaaccctgcg aactgacggt cactcgcggg
     1861 caatcgccat acacgctgac ctgggaatcg cctgcgcagc gatgtgatag gcgcagcctt
     1921 ccaagcagat gctcttgcag gtcaggcctg atatacaggc ctgatatagt cgacaccgat
     1981 gtggccccaa agcttagctc acctgtattc cgatgactta tggaccaaga gataactgat
     2041 cctatggtta ttgtacctcg gcgacctatc tgctaacagc aaaagccggt ctgtgagcca
     2101 agttgtttat cctactaggt tctgttgaca tttgtagagg accggctacc ttacaaaggt
     2161 ccgccagaaa gcccctcggc ttcagccagg ggatgaatgg cccgctgacc gcgctcggtg
     2221 gtatactgcc ggtgtaccgg ggggcacccg ggaacgatgg tggcacgggg acaccc
//
LOCUS       DASRJQ010000688         4168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_412218, whole genome shotgun sequence.
ACCESSION   DASRJQ010000688 DASRJQ010000000
VERSION     DASRJQ010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412218"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /gene="pyrR"
                     /locus_tag="VFB89_15510"
     CDS             <1..513
                     /gene="pyrR"
                     /locus_tag="VFB89_15510"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HZM28755.1"
                     /translation="DDRAIGRALARMASEVVERCGGTGELVLIGIQRRGVELAARICR
                     LIEQAEGAKVPVGKLDITLYRDDLQTVGPRPVVGETSLPDLDDRTVVIVDDVLYTGRT
                     VRAALDECADFGRPKRILLCVLVDRGGRELPIQADIVGSRASTGPDDRVDVFVSELDG
                     RDAVELVRNA"
     gene            524..1486
                     /locus_tag="VFB89_15515"
     CDS             524..1486
                     /locus_tag="VFB89_15515"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HZM28756.1"
                     /translation="MTAALGKDLVGLEYLSRDQIQLILDTAEPFKEVSERPIKKVPAL
                     RGKTIVNLFFEASTRTRISFEFAEKRLSADTVNVGSQGSSVQKGETLVDTAKNLEAMR
                     IDMVVIRHGASGAAQFLGSRIRSNVINAGDGKHEHPTQGLLDLLTLRDRLGRLEGIKV
                     AICGDVLHSRVARSNIWGLLKLGAEVGICGPATLLPRNIQDLGVTVLPRIEDAIQWAD
                     ALNILRLQLERMQAGFIPSLREYNRVFGVTTERLAKAPKDLVILHPGPVNRGVEIDSD
                     VADGPHSVILPQVTNGVAVRMAVLYLLAGGSPDWAQAAGKGEEH"
     gene            1483..2769
                     /locus_tag="VFB89_15520"
     CDS             1483..2769
                     /locus_tag="VFB89_15520"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HZM28757.1"
                     /translation="MSGILIRGGRVIDPARRLDEIADVLLLEGRVEAIGRNMGMPTGV
                     MVVDATGQVVAPGLIDLHVHLREPGQEDLETVATGALAAAAGGFSAVCAMPNTDPVCD
                     NQGVVGFMKAQAQRAGKARVYPIGAISLGQKGQQLAEFGELVGAGAVAVSDDGKPVVS
                     SHLMRTALEYARTFGIPVADHCEEPTLSAGGVMHEGIVSTRLGLKGIPAAAEEIMVAR
                     DLLLAELTGGHIHLCHMSTRGSVDLIRRAKDRGIRVTAEACPHHFALTHEACEGYDTN
                     AKMSPPLREPEDREAIRQGLKDGTIDVIATDHAPHHYDAKEREFDDAPNGIIGLETAL
                     GLAITELVKTGLLSLAELVERMSSGPARIFGLPGGTLAPGAPADVTVFDPDAEWTVHP
                     QEFYSRSRNTPFGGRRLTGRANVTIVRGQVAYQRPD"
     gene            2982..3965
                     /locus_tag="VFB89_15525"
     CDS             2982..3965
                     /locus_tag="VFB89_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28758.1"
                     /translation="MDDNNSAGSVPIEALLQERARFQQWLAKLDAAAGSAPAAVRQKV
                     RADYEGRLQTVIDQLKSHAATIQDELDRFRRSQQDLDGRKVGAEEALAEAQVRYAVGE
                     YSEEEWGRLNTDGSGTLETLQRELDQVETEIARLVEVQGLITAPAAEPVQAPEPIQAI
                     EPIHAPEPPAPSPQSRPAPPIEMLPDPTPDVAAASISSLPADPIPEIHPAPPAGAPRF
                     VPKPAAEAAQARPAAKPAAAKPSGPPLDELAFLKSVAGDKSAAGEDRKPRRSGETARP
                     EPVATSAPPAQPSAKPGQASQAKTLKCGECGTLNRPTEWYCERCGAELAAL"
BASE COUNT          767 a         1300 c         1388 g          713 t
ORIGIN      
        1 gacgaccgcg cgatcggccg tgccttggcg cgcatggcgt cggaagtcgt ggaacggtgt
       61 ggcggcacag gggagttggt gctcatcggc atccagcggc ggggcgtcga gctggcagcc
      121 cgcatttgcc gactgatcga acaggccgaa ggtgccaagg tccccgttgg aaagctcgac
      181 atcactctgt atcgcgacga ccttcagacc gtcggccccc gccccgtcgt cggggaaacc
      241 agccttccgg atctcgacga ccgtaccgtc gtcatcgtgg acgatgttct ctacaccggc
      301 cgcaccgttc gggcggcgct cgatgaatgc gccgacttcg gccggcccaa gcgaattctg
      361 ctctgtgtcc tggtagaccg aggggggcga gagcttccta ttcaggcgga tattgtgggg
      421 agtcgggcga gcaccggccc ggacgatcgg gtggacgtct tcgtgagcga gctggacggt
      481 cgtgacgcag ttgaactggt ccggaatgcg tgaggtgacc agtatgacgg cagcgctcgg
      541 gaaagacctg gttggcttgg agtatctaag ccgagaccag attcagctca tcctggacac
      601 ggccgagccc ttcaaagagg tgagcgagcg gcccatcaag aaggtcccgg ctctgcgggg
      661 caagactatc gtcaatctgt tctttgaggc ctcgactcgt acccggattt ctttcgagtt
      721 tgctgaaaag cgattgtcgg ccgacacggt caatgtcggc tcgcagggct cgtccgttca
      781 aaagggcgaa acgctggtcg ataccgcaaa gaatctggag gcgatgcgga tcgacatggt
      841 tgtgatccgg cacggcgcat ccggcgcagc tcagttcctc ggctcacgca tcaggtcgaa
      901 tgtcatcaat gcgggcgacg gcaagcacga acatcctacc cagggcttgc tcgaccttct
      961 tacgctacgg gaccgcctgg gccggctcga gggaatcaag gtcgcgatct gcggcgacgt
     1021 cctgcactcc cgggtagcgc ggagcaacat ctgggggctc ctcaagctcg gagccgaggt
     1081 cggcatctgc ggacccgcca ccctgcttcc ccggaacatt caggacctcg gcgttaccgt
     1141 ccttccccgg atcgaggacg ccatccagtg ggctgatgct ctcaatatcc tgcgacttca
     1201 gctcgagcga atgcaggcgg gatttatccc atccctgcgg gagtacaacc gggtcttcgg
     1261 ggtcactacc gagcgcctgg ccaaggctcc caaagatctc gtcatcctcc accccggccc
     1321 agtgaatcgc ggcgttgaga tcgatagcga tgtcgccgac ggcccccaca gcgtgatcct
     1381 gccacaggtg accaacggcg tcgcagtccg gatggccgtg ctctatctcc tcgccggcgg
     1441 gtcgcctgac tgggcgcagg cggcggggaa aggggaggag cattgagcgg cattctcatt
     1501 cgcggcggtc gggtgatcga tcccgctcgg cgactcgacg agatagccga cgtcctcctg
     1561 ctggagggtc gggttgaagc gatcggccgg aacatgggca tgccaactgg cgtgatggta
     1621 gtggacgcga cgggtcaggt ggtcgctccg ggattgatcg atctgcacgt gcatctgagg
     1681 gagccaggcc aggaagatct cgaaaccgtg gccaccggcg ccctggccgc ggcagcggga
     1741 gggttctccg cggtgtgcgc catgcctaat accgatccgg tctgcgacaa ccagggtgta
     1801 gtcgggttca tgaaggcaca ggcccagagg gcagggaagg cgagagttta tcccatcggc
     1861 gcaatctctc tgggccagaa agggcagcaa ctcgccgagt ttggtgagct ggtcggcgcc
     1921 ggtgcggttg ccgtgagcga cgacggcaaa cccgtcgttt cgagtcacct gatgcgtacg
     1981 gcgctcgagt acgcacggac cttcggaatt ccggtggccg atcactgtga ggagcctacg
     2041 ctgtcagccg gcggcgtgat gcatgagggg atcgtctcca caaggcttgg tctcaaaggg
     2101 atcccggcgg ccgccgagga gatcatggtc gctcgggatc tgctgctcgc cgagcttacg
     2161 ggcggacaca tccatctgtg ccacatgtcc actcgcggct cggtggatct gattcggcgg
     2221 gccaaggacc gggggattcg ggtgacggcg gaggcgtgcc cacaccactt cgcgctgacg
     2281 cacgaggcgt gtgagggata cgacaccaat gccaagatga gtccgccgct gcgagagcct
     2341 gaggatcggg aagcgattcg gcagggtctc aaggacggca cgatcgacgt cattgcaacc
     2401 gatcacgctc cccaccatta cgacgcgaag gagcgcgagt tcgacgatgc gcccaatggc
     2461 atcatcggtc tcgaaaccgc gctcggcctc gcgataactg agctggtgaa gaccggcctc
     2521 ctgtcattgg ccgaactggt ggaacggatg agctccggcc ccgcccgcat attcggactg
     2581 ccgggtggaa ccctggctcc aggtgccccg gccgacgtga ctgtgttcga ccccgacgca
     2641 gaatggacgg ttcaccctca ggagttctac tctcgaagcc ggaacactcc cttcggcggc
     2701 cgccgactga ccgggcgagc caacgtcacc atcgtacggg ggcaggtcgc ctaccaaagg
     2761 ccggactgag cggggcgatt cggccgcggc tttgcccgtt caccgtttac ccgcttttaa
     2821 ttagattttg cgacaggggt tatcggttcc atggcgagtg gagcgccgtg gtcacaccca
     2881 cccgccgatc gccagagcgg tccacttctg cccagggcga gtcaaccagc ccgagtcccg
     2941 atcaggtccg agcgcccgcg cgtttgggag gaatgtttgc gatggatgac aacaactccg
     3001 ccggcagtgt cccgattgaa gctctcctgc aggaacgcgc ccgattccag cagtggctgg
     3061 ccaagctcga tgcggcggcg ggctccgcgc cggccgccgt gcgccagaag gtgcgggcgg
     3121 actatgaggg ccgcctccag actgtcatcg accaactcaa gtcccacgcc gcgacgatcc
     3181 aggacgaact cgaccgattt cgccggtcgc agcaggatct ggacggacgc aaagtcggtg
     3241 cggaggaggc gctcgcggag gcgcaggttc gctacgcggt cggggagtac tcggaggagg
     3301 agtgggggcg gctcaacacc gacggcagcg gtacgttgga gacgctccag cgagagttgg
     3361 accaggtgga aaccgagatt gcgagactgg tggaggtgca agggctgatc acggcccccg
     3421 cggctgagcc ggtgcaagcg cccgagccaa tccaagcgat tgagccgatc catgctccag
     3481 agccgccggc gccgagccct cagtcgagac ccgctccccc gatcgaaatg ctgcctgatc
     3541 ccacgcccga tgtcgcggcg gcctcgatct cttcgctccc agccgatccc atacccgaaa
     3601 ttcaccctgc gcccccggct ggcgcgcctc ggttcgtgcc caagccggct gctgaagcgg
     3661 cccaggcgcg cccggcggcc aagcctgcgg cagcgaagcc gagtggtccc ccgctcgacg
     3721 agcttgcgtt tctcaagtcg gtcgcaggcg acaagtcggc agccggcgag gatcgaaagc
     3781 cccgccggag cggcgagacc gcccgccccg agccggtcgc tacgtcggct cctccggctc
     3841 agccctccgc caagccgggg caggcatcgc aggccaagac gctcaagtgt ggggagtgcg
     3901 ggacgctgaa tcggccgacg gaatggtatt gcgagaggtg cggggccgag ttggcagcac
     3961 tctagaaaaa gcgtgaacgg tgaacggtaa acggtgaacg gggtgtaggt taggacgctc
     4021 agctcaagta gcaacaggtt aatgagaacc cgaccaacgg aaacgggggt ccgataatta
     4081 tcggagcccc cgttcactgt tcaccgttca ccgttcacgc tgtttcaacg gctcaccgtt
     4141 cacgctgttt ctagcgcagt cctacttc
//
LOCUS       DASRJQ010000689         4160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_566127, whole genome shotgun sequence.
ACCESSION   DASRJQ010000689 DASRJQ010000000
VERSION     DASRJQ010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566127"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VFB89_15530"
     CDS             <1..625
                     /locus_tag="VFB89_15530"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZM28759.1"
                     /translation="MKAAIARADEILARLGDRGWMPKQFDNPANPAIHYQTTGPEIWA
                     DTDGKVDVLVSGIGTGGTITGAGRFLREKKPGIQLVAVEPNESPVLSGGQPSPHKQQG
                     IGAGFIPAILDTKIYNEVVRVTNDDAIATTRRLAREEALFVGISAGSITWAALQIAAR
                     PANAGKLIVSIIPDFGERYLSHPVFTELSDPDYSDLEEIVSRQPAAV"
     gene            complement(721..978)
                     /locus_tag="VFB89_15535"
     CDS             complement(721..978)
                     /locus_tag="VFB89_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HZM28760.1"
                     /translation="MGLIATLIIGLIVGVVAKLLMPGKDPGGFIVTTILGIAGAFVAK
                     FIGQAVGWYQEGQAAGFIASVVGAILLLAIYRLLTKGRATV"
     gene            1089..2468
                     /locus_tag="VFB89_15540"
     CDS             1089..2468
                     /locus_tag="VFB89_15540"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZM28761.1"
                     /translation="MSEHRYPLGHVLYRKLNKRFPLITRGEGCWLVDEEGKRYLDACG
                     GAFVANLGHGVAEIGQAMAEQAGRVAYVNGTAFTHEPVEQLATEVAALSPGDLNEVYP
                     LCSGSEAVEAAFKFARQYWVETGKAKKHKIVALTPAYHGNTLLALSASAREHYKTYYR
                     EWLVDVARVPAPYTYRCDCSGRPPLCPACDGSAVEEAIVRLGPETVAAFSAEPIGGSS
                     SGATVPPADYFRRIREICDRHQVLFVADEVLTGAGRTGTWAAISHFGVVPDLMTLGKG
                     LGGGYAPISALVAPGRLVDVLANGSGALLHAQTYSHHPVLCAGALAAVRYVQRHKLIE
                     RCAAMGQVLHRKLQALRGLPSVGDVRGRGLLAGVEFVADPETRAPFPRGRGFAERFTA
                     LALEEGLVVWPNVGQADGTNGDLVMLAPPFTISEEEISELVRRFSRALERAVAAGKAG
                     AAEKAVEAV"
     gene            2465..4042
                     /locus_tag="VFB89_15545"
     CDS             2465..4042
                     /locus_tag="VFB89_15545"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZM28762.1"
                     /translation="MTATAAEKITYTSASGDLDAFHRQFDATLAAVRAAAGKRHPCYI
                     AGRPVESKAEPLVDRSPIDTSFILGTFACANATHVDEAAKAAKAAQRGWARLSWQERV
                     ATMRQAARLVRERKYELAAIMALEVGKSRLEAMGDAEESADLIDYYAQQVEDADGFVR
                     PMGRVTPAERNTDVLRPYGVFTCIAPFNFPLALSAGMSSAALVAGNAVVYKPAEDTPW
                     TGLKLYEIYRDAGIPAGVFNVLFGHGTEIGDPLWQHPDVDGVVFTGSKAIGLRIHHGI
                     SERWIKPALLELGGKNAAIIMETADLDAAAEGVMRSAFGLQNQKCSATSRVYVHRSVA
                     QPFVDALLAKTAAMKMGDPSERDVYFGPVINEKAVARYEKAVAQARKDGTILTGGARL
                     SGGVFDKGHFVAPTIARLPLTSSLYTEELFVPFLAIGEVGSLDEALAETNKVEYGLTA
                     GIFSKRDEEVHRFFDEVEAGVCYANKRTGATTGAWPGAQPFCGWKGSGSTGKGGCGPY
                     YVQQFLREQSRTVIETA"
BASE COUNT          797 a         1257 c         1355 g          751 t
ORIGIN      
        1 aatgaaggct gcgatcgccc gtgccgatga aatcctcgcc cgtctgggcg accgggggtg
       61 gatgccgaaa caattcgaca atccggctaa cccggccatt cactatcaaa ccactggccc
      121 cgagatctgg gccgataccg atggcaaggt ggacgtccta gtgtccggga tcgggaccgg
      181 cgggacgatt acgggtgctg gaaggttctt gagggagaaa aagccgggga ttcaactggt
      241 ggcagtcgaa cccaacgaga gccccgtgct gtcgggaggg cagccgtcgc cgcacaagca
      301 gcagggcatc ggcgccggat tcatccccgc cattctcgac acgaagatct acaacgaggt
      361 cgttcgggtc accaacgacg acgcgatcgc cactacccgc aggctcgcac gtgaggaggc
      421 attattcgtc ggcatttcgg cgggttcgat cacgtgggct gctctgcaga tcgcggcccg
      481 cccggccaac gccggaaagc tgatcgtctc gatcattccg gacttcggcg agcggtatct
      541 ctcgcatccg gtctttaccg agctgtcgga tcctgattac tcggatctcg aggagatcgt
      601 ctcacggcag ccggcggcgg tttaaagact gcgcgaacgg taaacggtga acggtaaacg
      661 gggggtccct tgctgtggtg ccccccgttc actgttcacc gtttaccgtt cacgcttgtc
      721 tcagaccgtc gcccgccctt tggtgagcaa ccgataaatc gcgaggagga gaatcgctcc
      781 aaccaccgag gcaatgaagc cggccgcctg cccctcctga taccatccca ctgcctggcc
      841 gatgaacttc gcgacgaagg caccggcgat gccgaggatc gtcgtcacaa tgaatccgcc
      901 tggatccttg cccggcatca aaagcttggc aaccacgccg acgatcaggc cgataatgag
      961 cgtagcgatg agacccatcg atcctccttg aggtggtctc ccgaatgtat agtctttgta
     1021 gccgtctggt gactggcatt ttgtacaggt ccgggtcaca ttccggcttc acttcacgct
     1081 ccgccgacat gtccgagcac cgctatcctc tggggcacgt tctctaccgt aagttgaata
     1141 agcgcttccc cttaatcact cggggggaag ggtgctggct ggtggatgag gagggaaaac
     1201 gctacctgga cgcctgcggt ggggcgttcg tagccaacct cggccacggg gtcgccgaaa
     1261 tcggacaggc gatggccgag caggcaggcc gtgttgctta cgtcaacggc acggccttca
     1321 cgcatgagcc cgtcgagcaa ctcgccacag aggtggccgc tctatcgccc ggcgacctca
     1381 acgaggtcta ccccctgtgc agcggatcgg aggcggtgga ggcggccttc aagttcgccc
     1441 ggcagtactg ggtggagacc ggaaaggcga agaagcacaa gatcgtcgcc ctcactcccg
     1501 cctatcacgg caatacgctg ctcgcgctct ccgcctcggc ccgggaacac tacaagacgt
     1561 actatcgcga atggctggtg gatgtcgccc gagtaccggc gccgtacacc taccggtgtg
     1621 attgcagtgg ccggccgccg ctctgccctg catgcgatgg atctgcggtc gaggaggcca
     1681 tcgttcgact cgggcctgag acggtggccg cattcagcgc ggaacccatt ggtggttcgt
     1741 cgagcggcgc caccgtaccg ccggccgact atttccgccg aattcgagag atttgcgacc
     1801 gccatcaggt gctcttcgtc gccgacgagg tgctgaccgg cgccggacgc accggaacgt
     1861 gggcggctat ctcccacttc ggcgtggtac ccgatctcat gacactgggc aagggattgg
     1921 ggggcggcta cgcaccgatc tcggcactgg tcgcgcccgg tcgactggtt gacgtgttgg
     1981 caaacggctc aggtgccctc ctccacgcgc agacctattc gcatcatcca gtgctctgcg
     2041 ccggcgcgct cgccgccgtg cggtatgtgc agcgtcataa gttgatcgag cgttgcgccg
     2101 caatggggca agtgctgcac cggaagcttc aagcgttgcg aggcttgccg tcggttggcg
     2161 atgtgagagg tcgggggctc ctcgccgggg tggagttcgt tgccgaccca gagacaaggg
     2221 caccgtttcc ccgaggtcgg gggtttgccg agcggtttac cgcgctcgca ttggaggaag
     2281 gactggtcgt gtggcccaac gttggccagg ccgatggcac caatggcgac ctggtcatgc
     2341 tggcgcctcc atttaccata tcggaagagg aaatcagtga gctggtgcgg cgattcagcc
     2401 gcgcactgga gcgagccgta gcggcgggca aggccggtgc ggcagaaaag gctgtggagg
     2461 cggtatgacg gcaacggcgg cggaaaaaat cacgtatacc tctgcgtcgg gcgacctcga
     2521 cgcttttcac cggcaattcg atgcgacgtt ggcggcggta cgagccgccg ccggcaagcg
     2581 ccatccctgc tacatcgccg gacgaccggt agagtccaag gcggagccac tggtagaccg
     2641 gtcgccgatc gatacctcgt tcatcctggg cacctttgcc tgtgccaacg ccacgcacgt
     2701 cgacgaagcg gcgaaggccg ccaaggcggc gcagcggggg tgggcgcggc tgagctggca
     2761 ggagcgggta gccacgatgc gccaggccgc ccggctcgtt cgcgaacgaa agtacgagct
     2821 ggcggcgatc atggcgctcg aggtagggaa gagccggcta gaggccatgg gcgatgcgga
     2881 ggaatcggcg gacctgatcg attactacgc tcagcaagtc gaagacgcgg acggcttcgt
     2941 gcggccgatg ggacgggtaa caccggcgga acggaacacc gacgtgcttc gtccctacgg
     3001 cgtattcacg tgtattgctc ccttcaattt ccccctcgcc ctctcggcag gcatgtcgtc
     3061 ggcagcgctg gtggcgggga acgccgtggt gtacaagccc gccgaagaca ctccctggac
     3121 cgggctcaag ctgtatgaga tctaccggga tgctggtata ccagcggggg tcttcaacgt
     3181 cctgttcggc catggcaccg agatcggtga tccgttgtgg cagcaccccg acgtggatgg
     3241 ggtcgtgttc actgggtcga aagccatcgg cttgcggatc caccatggga tctcggagcg
     3301 gtggatcaag cccgcgctgc tcgagctcgg tggcaagaac gcggccatca tcatggaaac
     3361 ggcggatctc gacgcagcgg cagaaggcgt gatgcggtcc gcgttcggcc tgcagaatca
     3421 gaaatgcagt gcaacctcgc gcgtgtacgt gcaccggtca gtggcacagc cgttcgtcga
     3481 tgcgctgctg gcgaagaccg cggcgatgaa gatgggcgac ccgagcgaac gcgacgtcta
     3541 ctttggaccg gtgatcaacg agaaagctgt tgctcgttac gagaaggccg tcgctcaggc
     3601 tcggaaagat ggtaccattc tgaccggcgg ggctcgcctg agtggcggcg tattcgacaa
     3661 gggccacttc gtcgcgccga ccattgcccg actgccgctc acgagctcgc tctataccga
     3721 agagctgttc gtcccgttcc tcgcaatcgg cgaagtgggc agtctggatg aagccctggc
     3781 cgaaacaaac aaagtggaat atggcctaac tgccggcatc ttctccaagc gggacgagga
     3841 agtacatcgc ttcttcgacg aggtcgaagc gggcgtgtgc tacgccaaca agcgcaccgg
     3901 agccaccaca ggtgcctggc ctggtgcgca gccgttctgc ggctggaagg gatcggggtc
     3961 aacgggaaag ggcggctgtg gaccgtacta cgtgcaacag tttttgaggg agcaaagcag
     4021 gacggtcata gaaactgcgt aaacagtgaa cggtgaacgg taaacggggg gctccgatcc
     4081 accggaaccc cccgttcacc gtttaccgtt tacgcagttc agtcttccat tcccaggcat
     4141 tcatccaaaa ttccgagccc
//
LOCUS       DASRJQ010000690         2575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2387717, whole genome shotgun sequence.
ACCESSION   DASRJQ010000690 DASRJQ010000000
VERSION     DASRJQ010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2387717"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..389)
                     /locus_tag="VFB89_15550"
     CDS             complement(102..389)
                     /locus_tag="VFB89_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28763.1"
                     /translation="MELPGPARNVCLFSDGLERLVLDFRSRTAHAPFFDAVFRRFGGL
                     RQSGHAAQVSGELRELLRSDAVNQRTDDDKSIVCVSRVGNGNGAHRSTQEC"
     gene            480..647
                     /locus_tag="VFB89_15555"
     CDS             480..647
                     /locus_tag="VFB89_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28764.1"
                     /translation="MASNSRPHRMSGFYALFFLGNVLGVPLNLLLGQWAGGRLHVVGS
                     PTLISHAGGRG"
     gene            735..962
                     /locus_tag="VFB89_15560"
     CDS             735..962
                     /locus_tag="VFB89_15560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28765.1"
                     /translation="MLTDIEKRNADLDLIWKHTHPDFRGVIDGIRYVLTNRNGATVLA
                     PLHSLPEAELADSLAYARSRVERGLPAYSTT"
     gene            1192..1809
                     /locus_tag="VFB89_15565"
     CDS             1192..1809
                     /locus_tag="VFB89_15565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28766.1"
                     /translation="MSAAILLALCEYAWRDRQWIRFGLLGLLGIAAAVISGSVVLERV
                     SATSERATHAKRSDNLPRAEAQAALAEAQKALSKAEASTTAECSSGRGPRCTGLEKRE
                     DEARKRVEDARSKVTGLGAQTAENPAASLLGPWAATFQLAMLLGLPVWLELAAPVVLV
                     YGFAPLPSREEAPKRKAKRRKKKRHPRPPPAQPSAKVLPLKKAVP"
     gene            1924..2274
                     /locus_tag="VFB89_15570"
     CDS             1924..2274
                     /locus_tag="VFB89_15570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28767.1"
                     /translation="MPLYRGTRYALLIAGAVISVLAWDEEEGDATPDLVVTFDATVEA
                     GDVVRLQYDDEATFVAPTDTTEVLEAAEVAARQVAVALGELANGTYFARARIERGSAG
                     RSSWSNIEVITIAA"
BASE COUNT          412 a          907 c          817 g          439 t
ORIGIN      
        1 gcctccggag cacctcggcc ccgagctggt agacatcgcc ccgcagcgtg ccgagcaata
       61 ctggcggtcc cgctaccgac gcgcgcgggg ccagcgctga cctagcattc ctgcgtagag
      121 cggtgcgcgc cattcccgtt gcccacccga ctcacacaca cgatcgactt gtcgtcgtcg
      181 gttcgctggt tcaccgcgtc cgacctcagg agctctcgca gctcgcccga cacctgtgcc
      241 gcatggccgc tctgtcgcag cccgccgaac cggcggaaca cggcatcgaa gaatggcgcg
      301 tgcgccgtgc gcgaccggaa gtccagcacc aggcgctcga ggccatcgga gaacaggcac
      361 acattgcggg ctggccccgg aagctccacg acgcgctggt gcttagtgac ttcccctctg
      421 cctcaggacc accaggaagg gccgtccctg aggcggttgc tttgccagat aggtgcagca
      481 tggcttccaa ttcacgcccc caccgcatgt ccggcttcta tgccctcttc ttcctgggca
      541 acgtgctcgg cgtgccgctc aatctcctgc tcggccaatg ggctggtggc cgccttcatg
      601 tggtgggttc accgacgctg atctcgcatg ccggagggcg ggggtaaccc cgcccgaagg
      661 gcactcaaga ccattcccgc tttgtgactt tcccggatgc ctccggtgga cggttccaac
      721 cccggaggtg acccatgctg accgacatcg agaagcgcaa cgcagacctg gacttgatct
      781 ggaagcacac gcacccggac ttccggggcg tgatcgacgg tatccgctac gtgctgacca
      841 accgaaacgg cgccactgtg ttggccccgc tgcacagcct gccggaagcg gaactcgccg
      901 attcgctggc ctacgccaga tcgcgtgtcg aacgcggcct gcctgcctac tcgacgacgt
      961 gagccaatgg ctccggacgg ccctgcgggg ccgtcctccc gaggcagcca cctcgcctct
     1021 accccctcaa caacctttgg aacggcccgt aggagggggc caagacatgc tgacccgtgt
     1081 cttctgttag ctcgccgctt cgttgcaatc gccctgggcg ttgccctcac cgggtactcg
     1141 gcctgggtct cgtggcatca cacccatgat gtcctggggc cccttgcagc ggtgtcggcg
     1201 gccatcctgc tcgcgctctg cgagtacgca tggcgcgacc gccaatggat ccgcttcggg
     1261 ctgctgggcc tgctcgggat cgcggcggcg gtcatctccg ggagcgtggt gctggagcgc
     1321 gtctcagcca cgagcgagcg cgccacccac gcaaagcgct ctgacaatct acctcgagcc
     1381 gaggcacagg cagccttggc tgaggctcag aaggcgctgt cgaaggccga ggcctcgact
     1441 accgccgagt gcagctccgg tcgtggtccc cgttgcacgg ggctggagaa gcgggaggac
     1501 gaggcccgga aacgggtcga ggacgcccgc tccaaggtga ctggccttgg cgcgcagacc
     1561 gccgagaacc ccgcagcctc gctgctgggg ccttgggcgg cgacgttcca gttggccatg
     1621 ctgctgggcc tgcccgtgtg gcttgaactg gcagcccccg tcgtgctcgt ctacggcttc
     1681 gcgccactcc cctcgaggga ggaggcgccg aagcgcaagg ccaagcgacg caagaagaag
     1741 cggcatcctc gcccgccgcc agcccagcct tcggccaagg ttctcccctt gaagaaggca
     1801 gtgccttgaa tagccccctt tcggccttgc cgggccggag ggggcttttt gcgtctgcag
     1861 atccggcctc gacacgggcc gtcgtgctat actctgtccg cttcggatcg gaaccttgac
     1921 gccatgcccc tctaccgcgg aacccgctat gccctgctga tcgccggcgc ggtcatctcc
     1981 gttctcgcct gggacgagga ggagggcgat gccacgcccg acctggtcgt gaccttcgat
     2041 gccaccgtcg aggcgggcga tgtcgtccgg ctgcagtacg acgacgaggc caccttcgtc
     2101 gcgccgaccg acaccaccga ggtgctggag gccgcggagg tggcagcgcg gcaggtcgcc
     2161 gtggcgctcg gcgaactcgc gaacggcacc tacttcgccc gggcgcggat cgagcggggc
     2221 agcgccggcc ggtcctcgtg gtccaacatc gaggtcatca ccatcgctgc ctgacgccta
     2281 gaaagtgcgt tctctaacat caagcgccga acgcaaccga gtggagccag cgatcttcgg
     2341 cgctcgggta cgatgcagac acgtttggcc gattgtggcc acagggccga aggtctcggc
     2401 accgcaacag gtccgacagc tgtccagggg tacaacgggc gcgccgagag cgtcggcccc
     2461 atgcccgcat ttgacccatt cctgccgtct tcctcgatcc ctagcccgca tttctcacgg
     2521 aatctgatcc atcggggctc cgaatcagcg aaagcttgtt gtgcgacaac ggctt
//
LOCUS       DASRJQ010000691         2752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2097261, whole genome shotgun sequence.
ACCESSION   DASRJQ010000691 DASRJQ010000000
VERSION     DASRJQ010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2097261"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VFB89_15575"
     CDS             complement(<1..836)
                     /locus_tag="VFB89_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28768.1"
                     /translation="MLRPRPMRFLYLSVGLAIAFSSAVARAADSRLLSSCTHCWPLSG
                     VHPHSLMAPDGVGGTYVVWLDGSPRWRLQRLRADLTIPEPWPAAGLALNTEREASRIY
                     PVLAADGVGGVYVAWAEQATNGDMTAWLLRVRADGRPARGWPEGGVVLAPPSPMVMYP
                     ALARSGSGAAVAWFEGRNGAASIRLAARDANGRSLARWPEDGMMLPACESCTIPEAIQ
                     LASDGRGGLFVARAEMGARLSDLKMSHVSERAATARNWTLASAAVPPHAIQPDSHPEL
                     VA"
     gene            1042..2175
                     /locus_tag="VFB89_15580"
     CDS             1042..2175
                     /locus_tag="VFB89_15580"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZM28769.1"
                     /translation="MPGTTRPLESDANWHDVGAEAELTRKPIQQVVVDRVRIALVHKG
                     GEFTAISGVCNHVGGPLGEGVLEGDYVVCPWHQYRFHRRTGEGEPGYEEDRVPAHDIR
                     VEAGRVLVNVVPRTRRSKLHHPPHRLERIAPRGTPGGPPLDAPLRVVGISTTLMNEKY
                     PRFSTSEHLLEAGLAHAQRELSVETRLIRLRTLRFRPCEGYYSKSAHACTWPCSITQM
                     DPEDQLDRVYEAFVQWADIFLIATPIRWGSASSLYYKMVERMNCIQNQETIANRILLR
                     NKVVGFIVTGGQDNVQAVVGQMMTFFGELGCQFPQFPFIGHSLGWTAEDMERNVAVVQ
                     GSDELRVAACQLVARCVDTASALIGRRIGPERVSRGGRKAGHM"
     gene            complement(2254..>2752)
                     /locus_tag="VFB89_15585"
     CDS             complement(2254..>2752)
                     /locus_tag="VFB89_15585"
                     /inference="COORDINATES: protein motif:HMM:NF012676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HZM28770.1"
                     /translation="GLGQAISRGLAYAPYADLVWCETGKPDLAYARAFAEAIQARFPG
                     KMLAYNCSPSFNWKAKLDAATISRFQRELGAMGYKFQFVTLAGFHSLNHAMFDLAREY
                     RTRGMSAYSDLQSAEFAAEAQGYTATRHQREVGTGYFDQVMQLVSDGGCSTLALEGST
                     EKAQF"
BASE COUNT          477 a          927 c          911 g          437 t
ORIGIN      
        1 tccgccacca actccgggtg gctgtcgggc tgaatcgcat gagggggcac cgcggccgaa
       61 gccagagtcc agtttcgggc agtcgcggcg cgctccgaga cgtgggacat cttgaggtcc
      121 gacagtcgcg cgcccatctc cgccctcgcc acgaagagtc cgcctcggcc gtcgctggcg
      181 agctggatgg cctccgggat tgtgcatgac tcgcacgctg gcagcatcat gccgtcctcc
      241 ggccaacggg ccagcgatcg gccattcgcg tcgcgagcgg cgagacgaat cgaagccgcc
      301 ccgtttcgcc cttcgaacca ggcgaccgcc gctccgctcc ctgaccgcgc cagagccggg
      361 tacatgacca tgggactcgg cggcgccagg accactcccc cctcgggcca tccgcgtgcc
      421 gggcggccat cggcacgcac ccgcaacaac catgcggtca tgtccccgtt ggtcgcttgc
      481 tccgcccagg cgacgtagac cccaccgacg ccgtcggccg ccaggacggg atagatcctg
      541 ctcgcttcgc gttcggtgtt gagcgcgagc ccggcggcgg gccatggctc cggaatggtc
      601 agatcggcac gcagccgctg gagccgccag cgggggctcc catccagcca gacgacgtag
      661 gtgcctccca cgccgtcagg cgccatcagc gagtggggat gcacgccgct cagcggccag
      721 caatgcgtgc acgaggaaag caggcgagaa tcggcggccc gagcgaccgc ggatgagaag
      781 gcgatcgcca agccgacgga gaggtacagg aagcgcatgg gacgtgggcg gagcatgagg
      841 gcctcgagcc tggtcccgaa gtcggctgca taaaatgatc cactcggcaa tggccggcgc
      901 caagagcaat tatgagcatg ggcgctggaa ataggggctg caaccgcccg gggtgggcca
      961 gaggcgggtg cgttttgccc gcctacgccc tggcagtagg ctgcgcgggc acggcctggt
     1021 gcggatctac ggagacttgc gatgcccgga acgactcgcc ctctcgaaag cgacgccaac
     1081 tggcacgatg tcggcgccga agccgaactc acccgaaaac caatccaaca agtcgttgtc
     1141 gatcgtgtcc gcattgccct ggtgcacaag ggtggcgagt tcaccgccat ttccggggtg
     1201 tgcaatcacg ttggcggtcc gctgggcgaa ggcgttctcg agggcgacta cgtggtctgc
     1261 ccctggcacc agtaccgatt ccatcggcgc acgggggaag gggagcctgg ttacgaggag
     1321 gatcgcgttc ccgctcacga cattcgggtc gaggctggcc gcgtgctggt gaacgttgtc
     1381 ccacgcaccc gtcgcagcaa actccatcac ccgccgcacc ggctcgagcg catcgccccc
     1441 cgaggcacac ccggcggtcc gccgctcgac gcaccgctac gtgtggtcgg aatctcgacc
     1501 acgctgatga acgagaagta cccgcgcttt tcgacttccg aacacctgct cgaggccggg
     1561 ctcgcacacg cgcagcgtga gttgagcgtc gagacccggt tgatccggct gcgtaccctg
     1621 cggttccgcc cgtgcgaagg ctattactcg aagagcgccc acgcgtgcac ctggccatgc
     1681 tcgatcaccc agatggatcc cgaggatcag ctcgaccgcg tttacgaggc cttcgttcag
     1741 tgggccgaca tcttcttgat cgccactccg atccgctggg gctcagccag ttcgctctac
     1801 tacaagatgg tcgagcggat gaactgcatc cagaaccagg agacgatcgc gaaccgtatc
     1861 ctgctccgca acaaagtggt cggcttcatc gtcaccgggg ggcaggacaa cgtccaggcc
     1921 gtggtcggac agatgatgac attcttcggc gagctgggct gccaatttcc tcagtttccg
     1981 tttatcggcc attccctcgg ctggaccgcc gaggacatgg aacggaacgt tgcggtcgtc
     2041 cagggaagcg acgaactccg cgtggccgcc tgtcaattgg tggcgcgctg cgtggacaca
     2101 gcgagcgcat tgatcggtcg tcggatcggc cccgagcgcg tatcgcgagg cggacgcaag
     2161 gccggccaca tgtagcccgc gtcctgccca tcaaacggga aaccccgcgg gctgacggct
     2221 cccgcggggt ggcgtgcctg cgccgtttgc ccgctagaac tgcgccttct ccgtcgatcc
     2281 ttccagcgcc agcgtcgagc agcccccgtc ggagaccagc tgcatgacct ggtcgaagta
     2341 cccggtgccg acctcgcgct gatggcgcgt ggcggtgtag ccctgggcct cggccgcgaa
     2401 ctcggcgctc tgcagatcgg aataggccga catgccgcgc gtccgatact cgcgcgccag
     2461 gtcgaacatg gcgtggttga gactgtggaa gccggccagg gtcacgaact ggaacttgta
     2521 gcccatcgcg cccagctcac gctggaagcg cgagatggtg gcggcatcga gcttggcctt
     2581 ccagttgaac gagggtgagc agttgtaggc cagcatcttg cccgggaacc gcgcctggat
     2641 cgcctcggcg aaggcccgcg catatgcaag gtccggcttc ccggtctcgc accacacgag
     2701 gtcggcgtat ggcgcgtagg ccagaccgcg cgagatggcc tgccccaggc cc
//
LOCUS       DASRJQ010000692         2546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1391630, whole genome shotgun sequence.
ACCESSION   DASRJQ010000692 DASRJQ010000000
VERSION     DASRJQ010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1391630"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VFB89_15590"
     CDS             complement(<1..593)
                     /locus_tag="VFB89_15590"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZM28771.1"
                     /translation="MTERVIETHRLRKRFGSKLAVADLSLTVARGEVFGFLGPNGAGK
                     TTSLKMLLGLIEPSAGSASVLGAPLGNRLARSRVGFLPEHFRFHEWLTGRELLGFHGR
                     LHGLSGGQLRNRIEDLLERMDLLEAGDRPVRAYSKGMMQRVGLAQALLNEPSLVFLDE
                     PTSGLDPLGRLLVRDVIHELKAQGTAVFLNSHLLGEI"
     gene            complement(590..1573)
                     /locus_tag="VFB89_15595"
     CDS             complement(590..1573)
                     /locus_tag="VFB89_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28772.1"
                     /translation="MDDRFMMGLKRTPDPEFARRLRDRLRLAEGAEEEDGGKQPARWG
                     VVLAPTLAVALLASVILFPSVRASAQAFLDLFRVRTFTAVSVSQERLAKLREQKFDFK
                     TLIGERVEMIREPGDPQVVSSPAQAGAAAGFTVLVPTLLPGDLRADSVAWRGPGEARV
                     TPDVARLRSLLETLDIRDLTVPVGLGDQPIDIKLPALVKQRFRGSKGHVEFLQARSPE
                     VGLPAGVDMAALGEIGLRVLGVQPAEARRLAQTIDWRTTMLIPVPSNAGSFRQVQVGG
                     QPGLMVTLVITNPEGRRREGAMVLWSRGDMVYGMQGNISGIDLLQMANSLQ"
     gene            complement(1576..2121)
                     /locus_tag="VFB89_15600"
     CDS             complement(1576..2121)
                     /locus_tag="VFB89_15600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZM28773.1"
                     /translation="MASETAVLQRLIAWVAPAEVDWDAAYREQLPRVYNFFRYRVGPG
                     PEAEDLTARTFEKAWVARERYRPDLGRFETWVMTIARRVAIDHLRGRRLHLPLEAAED
                     LAVGPGPEDLAESRSDQERLVRLLAGLGDRERELVALKYGAEMTNRAIARHTGLSESN
                     VGTILHRTVQQLRAEWGEGGG"
     gene            complement(2214..>2546)
                     /locus_tag="VFB89_15605"
     CDS             complement(2214..>2546)
                     /locus_tag="VFB89_15605"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HZM28774.1"
                     /translation="GRCEIRGRFDRIDARSQDGGIEADLLPGSRVPQDGWSLRSQDGG
                     ILLRIPHDLVATLDAQTQDGSLNVDLPVRIQGGRVFRHQVVADLNGGGPTVRLRTSDG
                     SIRVAAID"
BASE COUNT          411 a          913 c          846 g          376 t
ORIGIN      
        1 tcgatctcgc ccagcaggtg cgagttgagg aagaccgcgg tgccctgggc cttgagctcg
       61 tggatcacgt cgcgcaccag cagccggccc agcgggtcga ggccggaggt cggctcgtcc
      121 aggaacacca gcgagggctc gttcaggagg gcctgtgcca ggcccacgcg ctgcatcatg
      181 cccttgctgt aggcgcgcac cggacgatcc ccggcttcga gcaggtccat gcgctccagc
      241 agatcctcga tgcggtttcg cagctggccg ccggaaagtc catggaggcg accgtgaaag
      301 cccagcagct cgcggccggt gagccactcg tggaaccgga agtgctcggg caggaatccc
      361 acgcgcgagc gcgcgagccg gttgccgagc ggggccccca gcaccgacgc gctcccggcg
      421 ctgggctcga tcagtcccag cagcatcttg agcgacgtgg tcttgcccgc cccgttggga
      481 cccaggaagc cgaacacctc tcctcgagcg acggtcaagg agaggtcggc gacggcgagc
      541 ttcgagccaa aacgctttcg aagccggtgg gtctcgatga cccgctcggt cactgcagcg
      601 agttcgccat ctggagcagg tcgatgccgg agatgttgcc ctgcattccg tacaccatgt
      661 cgcctcgcga ccagagcacc atcgcgccct cgcgccggcg accctccggg ttcgtgatca
      721 ccagggtgac catcaatccc ggctgtccgc ccacctggac ctggcggaag gagccggcgt
      781 tgctcggcac gggaatcagc atcgtggtgc gccagtcgat cgtctgcgcc agacgtcggg
      841 cctccgccgg ctgcacgccg agaacccgca atccgatctc gcccagcgcc gccatgtcca
      901 ctccggccgg caggccgacc tcgggactgc gcgcctgaag gaactcgacg tgacccttgc
      961 tgccgcggaa acgctgcttg accagcgccg gcagcttgat gtcgatcggc tggtcgccga
     1021 gccccaccgg gacggtgagg tcgcgaatgt ccagcgtctc gagtaacgag cgcagccgcg
     1081 cgacgtcggg ggtcacgcga gcctcgccgg ggccacgcca ggccaccgag tcggctcgca
     1141 ggtcaccggg aagcagggtg ggcacgagga ccgtgaaacc ggccgcggct ccggcctggg
     1201 ccggcgaact gaccacctga ggatcaccgg gctcgcgaat catctccacc cgctcgccga
     1261 tcaacgtctt gaaatcgaac ttctgctctc gcagcttggc cagccgctcc tggctcacgc
     1321 tcacggccgt gaaggtgcga acccgaaaga ggtcgagaaa cgcctgggcg gaggcccgca
     1381 ccgagggaaa gaggatcacg ctggcgagca gagccacggc cagggtagga gcgagcacga
     1441 caccccatcg cgccggctgc ttcccgccgt cttcctcctc agccccttcc gcgagccgca
     1501 accggtcgcg cagtctgcgc gcgaactccg gatcgggggt gcgcttgagc cccatcatga
     1561 aacggtcgtc catcgtcatc ctcccccttc gccccattcc gcgcggagct gctgcaccgt
     1621 gcgatgaaga atggtgccga cgttcgattc actgagtccg gtgtgccgcg cgatggcccg
     1681 gttggtcatc tccgccccgt acttgagcgc caccagctcg cgctcccgat cgcccaatcc
     1741 cgccagcagg cggaccaggc gctcctggtc cgagcggctc tccgccagat cctcgggacc
     1801 aggccccacg gccaggtcct cggccgcctc gagcggcaga tgcaggcggc ggccgcgcag
     1861 gtgatcgatc gccacccggc gggcgatcgt catcacccag gtctcgaagc gcccgagatc
     1921 cgggcggtat cgttcgcggg ccacccaggc cttctcgaag gtcctcgccg tgaggtcctc
     1981 ggcttccggc cccgggccga ctcgataacg gaagaagttg tagacacgcg gaagctgctc
     2041 gcggtaggcg gcgtcccagt ccacctccgc gggtgcgacc catgcgatca gccgctgcag
     2101 caccgcagtc tcactggcca tgtactgcac tatacgaggc tccggcccag gtatcacaag
     2161 ccggggaggt cggccgtccg ggcgtggcgt ggatcccgaa gtgcctgtgc cgcctagtcg
     2221 atcgcggcca cccggatgga gccgtcgctg gtgcgcagcc ggacggtggg gccaccacca
     2281 ttgaggtccg cgaccacctg gtgacggaaa acgcggccgc cctggatccg gaccggaagg
     2341 tccacgttca ggctgccgtc ctgggtttgc gcgtcgagcg tcgcgaccag gtcgtgggga
     2401 atccgcagca ggatgccgcc gtcctgcgag cgcagtgacc agccatcttg cggcactcgc
     2461 gagccgggca acagatccgc ctcgatgccg ccgtcctgac tccgggcgtc gatgcggtcg
     2521 aagcgcccgc ggatctcgca gcgccc
//
LOCUS       DASRJQ010000693         2868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3080637, whole genome shotgun sequence.
ACCESSION   DASRJQ010000693 DASRJQ010000000
VERSION     DASRJQ010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3080637"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VFB89_15610"
     CDS             complement(<1..302)
                     /locus_tag="VFB89_15610"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HZM28775.1"
                     /translation="MRSRIRCFALAMGLLAAAGCAGQQVSADYSPSVGFSQYRTFAIV
                     SRPDSASHQLLDDRVRSAIEAQLPTKGLTETDRGSADLYIGYGIVDRTHKEVYTTET"
     gene            complement(387..1316)
                     /locus_tag="VFB89_15615"
     CDS             complement(387..1316)
                     /locus_tag="VFB89_15615"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZM28776.1"
                     /translation="MILVTGSTGLIGSEVVRLLSQAGVPARALVRNAASGDELPGITW
                     VIGDLAKPETLRSVFAGCTKLFLLTGNSQNAYELQRHALAAARQAGVTQVVKLSAFGA
                     SPHSNSTIGRMHYQIESELQGSGLAWTMLRPHHFMQNLLGQADNIINQGVVYSSSGDG
                     RIPFVDTRDIAAVALVTLTQPGHLEKKYVITGSEALSYRQATDILGRAIGRQLRFIDE
                     PSDDARARLTKAGQPGWLVDSLLAIAAYQRAGGPTETITSVVADLTGKPPRTFAEFAR
                     DYASAFLGGIAKTPRDVSPSLREGLTRSQSSLR"
     gene            complement(1325..2191)
                     /locus_tag="VFB89_15620"
     CDS             complement(1325..2191)
                     /locus_tag="VFB89_15620"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HZM28777.1"
                     /translation="MLLHSDLPLEWAGLAAVAAGIKVAGVVYARGWHRYRLRLPGRFG
                     VPRLAAFLGGLACLCAAILPPLDEAADTRLSAHMMQHILLLTVSPALLLLGDPLLLLV
                     RGLPDSFRRTFVAGLARQRWLRRAMHSLVHPLVALLVSSVIFWSWHVPALYQLALRVP
                     AIHVVEHVSFFAGGLLFWYPVVQPWPSRPKWPTGAVIPYLLAADVQNTVLAALLTFSD
                     RVLYPFYEFHSRAAEAAALDDQVLAGVIMWVPMSLAYLVPAALLTIRLLSPAKPLSRR
                     TVGLALSRTRTS"
     gene            complement(2197..>2868)
                     /locus_tag="VFB89_15625"
     CDS             complement(2197..>2868)
                     /locus_tag="VFB89_15625"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZM28778.1"
                     /translation="AVLVCAAVFGPFGPSGRPDPRMIDIAPKPDVFFLWLYAALALLP
                     AELETALILIVPVVAIAVLLLLPFISGTGEKSWKRRPFAVLVVVLLASGFIALTWLGL
                     QPPWSPSMTAWSGTPLPPRLLKERTPLELQGALVFQNKQCRNCHALGGTGGDRGPPLD
                     NVAVHLTRDQLIRQVLQGSGEMPAYGDHLHPAEVAALVSYLETLHPPYQPAARVSAAS
                     SGSSP"
BASE COUNT          540 a          903 c          968 g          457 t
ORIGIN      
        1 gtctccgtgg tgtacacctc tttgtgggtg cgatctacga tcccgtaccc gatatacaga
       61 tcggcgctcc cgcgatcggt ctctgtgagg cccttcgtcg gtagctgcgc ttcgatcgcg
      121 ctgcgcacgc gatcatcgag caactggtgg gaggcgctgt ccggccggga gacgatcgca
      181 aacgtccggt attgcgaaaa cccgaccgat ggactgtagt cggccgagac ttgctggccg
      241 gcacagccgg ctgctgccag caggcccatg gccaatgcaa aacagcgtat cctactccgc
      301 atctccgact cctttgggga ggttcccctt cgttgcctgc ccggcgcccg ggagggcaag
      361 ccgagtgcca ctgataagtc cttgattcaa cgaagtgatg actgggatct cgtcaaaccc
      421 tcacgaaggc tcggggaaac gtcgcgagga gtcttcgcga ttccgccaag aaatgcgctg
      481 gcgtaatcgc gggcgaactc cgcgaaggta cgcggtggct tgccggtcag atccgcgaca
      541 acactggtaa tcgtctcggt cggcccgccg gcgcgctggt atgccgcgat tgcgagcagg
      601 ctgtcgacca gccagccggg ctggccggct ttcgtgagac gtgcccgcgc gtcgtcggac
      661 ggctcgtcga tgaaacgcag ttggcggcca atcgctcggc cgaggatgtc cgtcgcttgc
      721 cggtacgaga gtgcttcact cccggtgatg acgtacttct tctcgaggtg ccccggttgg
      781 gtcagggtga cgagggcgac cgccgcaatg tcgcgggtgt cgacgaaggg aatcctgccg
      841 tcgccggaag aagagtagac gacaccttgg ttgatgatgt tgtcagcctg gcccaggagg
      901 ttttgcatga agtggtgcgg ccgaagcatg gtccacgcca gtcccgatcc ttgcagctcg
      961 ctctcgatct gatagtgcat gcgaccgatc gtggaattcg aatggggcga cgcgccgaac
     1021 gcggaaagct tcaccacctg agtgaccccc gcctggcgag ctgccgcaag ggcgtggcgt
     1081 tgcaactcgt atgcgttctg gctgttgccg gtgagcagaa acagtttggt gcaacccgcg
     1141 aacaccgagc gtagagtttc cggcttcgcg agatctccaa tcacccacgt tatgccgggt
     1201 aactcgtctc cgctggccgc gttgcggacc agcgcacgcg cgggcactcc ggcctgcgag
     1261 agtaaccgta ccacttcact tccgatcaga ccggtgcttc cagtgacgag aatcatgatt
     1321 ggcctcagga ggtgcgtgtt ctggaaagag caagccccac cgttcgcctg ctcagcggct
     1381 tcgccggcga cagcaggcgg atggtgagca gcgccgcggg gaccaggtac gcgagcgaca
     1441 tcgggaccca catgatgacg ccggccagca cctggtcatc gagtgcggct gcttccgctg
     1501 cccggctgtg gaactcgtag aacggataga gcacccgatc ggagaacgtc agcagcgccg
     1561 cgagcacggt gttctgcacg tcggcagcca gcaagtacgg aatcacggcc ccggtcggcc
     1621 acttggggcg actcggccag ggctgcacaa cggggtacca gaacaggagt ccccccgcaa
     1681 agaacgacac atgctccacc acgtgaatgg caggcacgcg aagagcgagc tggtacaggg
     1741 ccggcacgtg ccagctccag aagatcactg agctgaccag aagcgccacg agtggatgca
     1801 cgagactgtg catcgcccgt cgaagccacc gctggcgcgc cagcccggct acgaatgtcc
     1861 gccgaaatga atcgggaaga ccgcgcacga gcagcaggag tggatcgccg agcaggagga
     1921 gggcagggga cacggtgagc agcaggatgt gctgcatcat gtgcgcgctc agcctggtgt
     1981 ccgctgcctc atccagtggg gggagaatcg cggcacacag gcacgcgagg cctcccagga
     2041 aggcggcgag tcgcggtacg ccgaagcgcc ctggcagcct gagacgatag cggtgccagc
     2101 cccgagcata gacaaccccg gcgaccttga tgccggccgc caccgcggcc agccctgccc
     2161 attccagcgg caggtcgctg tgcagcagca tcggggctac gggctcgagc ccgacgacgc
     2221 ggccgacacc cgggccgccg gctgataggg cggatgcagg gtctcgaggt aggacaccag
     2281 ggccgccact tctgccgggt gcaggtggtc accgtacgcc ggcatctcgc cgctgccctg
     2341 gagtacctgc cggatcagtt gatccctggt caaatgcacg gcgacgttgt cgagcggggg
     2401 tccccggtcg cctccagtcc cgccgagtgc atggcagttc cggcactgct tgttctgaaa
     2461 caccagggcg ccctggagct cgagcggcgt tcgctctttc agcagccggg gcggcaatgg
     2521 cgttccactc caggcggtca tggagggcga ccagggtggc tggaggccga gccacgtgag
     2581 cgcgatgaag ccgctggcga ggagcaccac cacgagcacg gcgaacggcc gccgcttcca
     2641 gctcttctct ccggtgccgg aaatgaacgg caggagcagc agcaccgcga tcgctaccac
     2701 agggacgatc agaatcagcg cggtctcgag ctccgcgggg agaagggcca gcgcggcgta
     2761 gagccagaga aaaaagacat cgggcttggg cgcgatgtcg atcatccttg gatcgggccg
     2821 gcccgacggg ccgaacgggc cgaatacggc ggcgcaaacc aggaccgc
//
LOCUS       DASRJQ010000694        13191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2389835, whole genome shotgun sequence.
ACCESSION   DASRJQ010000694 DASRJQ010000000
VERSION     DASRJQ010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 13191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13191
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2389835"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VFB89_15630"
     CDS             <1..481
                     /locus_tag="VFB89_15630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28779.1"
                     /translation="SLNPSQISGGCGRLLCCLKYEHEFYVTARKRFPKEGKSLQTSLG
                     IEKVIAVDIFRERVFLRSEEHGSRIIPLMQLREEMDRAVALLAVPDTRTRQAPPTSEP
                     APTVPPVQAISPTPGNGNAPASGNGHANGVHPTRRRRRRRGPGDTGGSTGSPPPVEP"
     gene            482..1996
                     /gene="metG"
                     /locus_tag="VFB89_15635"
     CDS             482..1996
                     /gene="metG"
                     /locus_tag="VFB89_15635"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HZM28780.1"
                     /translation="MPKFYLTTAIDYSNGEPHLGHALEKIGADCIARYRRLRGDSVHF
                     VMGMDENAQKVAQAAERAGVPTAEWVRSLAERFDATWRRLLCSHDDWIRTTEPRHHRA
                     TIALIERIAERSPDDLYSGLYEGLYCIGCEEFKQEAQIINGHCEEHPNLELIHTKEQN
                     HFFRLSRYRDEVLRLIQSGELSVEPTIRRNEVLRVLEAGLQDISISRQRLSWGIPFPG
                     DSSQTVYVWFDALINYLSATGFPEAGYERLWPADLHVIGKGISRFHCIIWPAMLLSAG
                     VALPRKVWAHGYVQWSGAKVSKSAGTAVSLDEAIDRHGPDALRYFLLREVGFEADGNF
                     TWERFDERYTADLADGLGNLASRTLAMLGRYRDGKVPDAAPATRLHDAGSRCLSRYAA
                     AMDRNDLRGGAEAAWELVSEANLYIQQTAPWTLAKEGREAELDAALAALAACLYRLAV
                     LVTPFVPGKAQTLWQALGQRGDSTTAQWTSLEAPRVAGTLTTKPEVLFPKPTPT"
     gene            2257..3183
                     /locus_tag="VFB89_15640"
     CDS             2257..3183
                     /locus_tag="VFB89_15640"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZM28781.1"
                     /translation="MAKHRWTIVLVPHGAEPSKVLELSWGAVKLAGALAVALVVVALL
                     VGTATVSRRVDLSRTARLEDENQRLALELGELHGRLTTLSDTIARISQRDAKIRLLAN
                     LEPISPQVQAAGIGGPALSTSLPAHVSSALLRKTADVRLDVNALIRRANLLASSFSAA
                     ADSLASHTDRMSATPSIMPTQGWLSSAFSAMREHPILHVARPHEGIDVSAPMGTPIEA
                     PANGVVRSTGWEAGYGNVVEIDHGFGIVTRFAHTSRILVRPGQRVQRGDRIALVGNSG
                     LATGPHLHYEVHVNGRAVDPLRYVMPESVVTD"
     gene            complement(3180..3749)
                     /locus_tag="VFB89_15645"
     CDS             complement(3180..3749)
                     /locus_tag="VFB89_15645"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HZM28782.1"
                     /translation="MMRSRWWSPATLLGAALLLTGCGYNRMQSMDERVNQAQGQIQTQ
                     LQRRADLIPNLVNTVKGVTKQEDTVFISIANARARLSGAVQAGNVQDMAAANDQLSAG
                     LGRLLAISENYPQLRSSENFRQLQDQLEGTENRISVARTDYNTAAGEYNAFIRRFPYN
                     LTAKIFGLDKPRQYFAAQAGAQEAPKVQF"
     gene            complement(3746..4465)
                     /locus_tag="VFB89_15650"
     CDS             complement(3746..4465)
                     /locus_tag="VFB89_15650"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HZM28783.1"
                     /translation="MNDGIRRLVDPFLADVDAAIGTTYSAILFGSAARGDYVSGASDV
                     NLMLIVERAEPEDLARLRPAFARWRKATAGLPLVISREEWLEGVDVFPIEICDLRAAH
                     QVLRGPDLVAPVEPGRADLRRALEKEFRGKLLRLRQRYVAHGEENLGSWAGGTIASIV
                     LFFRCLLSLTGRPAPAEPSRVIEDAATLLGFEPAPLLRVLACRTAAKPTLAASDFEAY
                     IRAVERAAQFTDQLELGDLHS"
     gene            complement(4465..5292)
                     /locus_tag="VFB89_15655"
     CDS             complement(4465..5292)
                     /locus_tag="VFB89_15655"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HZM28784.1"
                     /translation="MTRHKAIVAAMALQLAASAAGAQQDAVSRLFPARPTGYVTDVAG
                     IVDPAEKAQLESLISRLRQATGAEIAVVALPTLGDRSESEVALAILRSWGVGRKAEIG
                     DSTRNAGLVLLFVPRQEGKPGSGAIRIEVGYGLEGIVTDAAAGRVRDLMLPAAREGNY
                     SQAIDAGVRALVGLVARGYGVTDSTLAAASPHESRGGNSGIPFWLPLVLMLFFFFLSS
                     RFGGRRRRIYWGGGPWIGGGWGGGGGWGGGGGGFGGGGFGGFGGGGGGGGGGAGGRF"
     gene            5286..6029
                     /locus_tag="VFB89_15660"
     CDS             5286..6029
                     /locus_tag="VFB89_15660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28785.1"
                     /translation="MSRLWYVLALAVVACERPNDVAVRVSIPGPDSVSAPVPGAGLIA
                     LPYDRDSVLRALATAARSPRPSTKELDSLFQQFRIPFSNYAKAGYEAEQLRRSQDSLK
                     SRLDSLPRNAPEYRSLYARFGEQSSALSAAEKREKSARTALDAARATFVARSDTLRAR
                     VRRWEDSTYQGYDSIVRELSRARGMTAVTDTTGADGWAHFHLQRGPWWIYARSWDASD
                     ANAEWYWNVKVTGDTVLLDSHSGTRRPKY"
     gene            6056..6709
                     /locus_tag="VFB89_15665"
     CDS             6056..6709
                     /locus_tag="VFB89_15665"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZM28786.1"
                     /translation="MGPSSRALALLTLVSSVLPAILAAQGSSQNLLAERSKGSATAPI
                     TVYEMSDFQCPYCRQHAVETFPELEKSYIQTGKVRWVFLNFPLTSIHHNAAAAAELAL
                     CGARQGKFWPLHDLLFKYQQAWAPLKEPGPFLVTLIDSVNAPRAPMEACLTAGAARPE
                     LEADAQAAQRSGAQSTPTFYIEGGLLVGAQPVALWKEILDSVYQAKKSVNGEEKRNR"
     gene            complement(6767..7480)
                     /locus_tag="VFB89_15670"
     CDS             complement(6767..7480)
                     /locus_tag="VFB89_15670"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HZM28787.1"
                     /translation="MSAYWRLSRSPRYSLLFALPLLLLYEALAFVLSHDSLTGVRNGA
                     DVLLKSVFVWLGGRIGLIVFGLFLLGGGAVLIRRDRSGGKLRPQLFLLMAIEAGVYAG
                     LFGIVTGILTGWLLGGLRLAIAPAVALDLPTQLMVSLGAGIYEELLFRVLIVGALAWL
                     AKRLFGWGPVAAGLFATMTGALIFSLFHYVGPYGDELRLGSFAFRAVAGVLFSGLYLT
                     RGFGITAWTHALYDVLVTI"
     gene            complement(7844..8227)
                     /locus_tag="VFB89_15675"
     CDS             complement(7844..8227)
                     /locus_tag="VFB89_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28788.1"
                     /translation="MSARLARVMLALTPIILGGCSLNFDATSLGVPVTMASPAGQAAP
                     VDSFRVSSHAIWAFWGIMGLKRPALNKSLASQLAGGKGIADLKIKTHASFTDVLFTII
                     TAGVIVPRSVVYEGVVTQTAPVKTQ"
     gene            complement(8224..8889)
                     /locus_tag="VFB89_15680"
     CDS             complement(8224..8889)
                     /locus_tag="VFB89_15680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28789.1"
                     /translation="MRPSHRALGLALALTLGAASIGSAQALGLPVYNSGVPTGLGIYG
                     DVAFPDGAAGDGYTLGLSGRLGWSKLGVTATIASFNPSGADNTLTTWGATGNYKLIGG
                     PLVPFSATVQGGVGYGRVGPDSTGVKTWHFPIGLGLALTIPNPALAIKPWIAPRIDIT
                     ETSVNDGPSDTDSNFGMSAGIEFNFLNGFGLQTAYDRVFVNGDDPGIFSVGAHYAFRI
                     PGL"
     gene            complement(9035..9652)
                     /locus_tag="VFB89_15685"
     CDS             complement(9035..9652)
                     /locus_tag="VFB89_15685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28790.1"
                     /translation="MWRSARIGFAALALMSAATGTLAAQETATPVFKAPYRAFTSHEF
                     GGNLSFPSGDVNLAVEGFYGFGHGAYDIGIRGGFEDLEGSGDTRILLGGDFRTRVLKA
                     SEQFPFDGALTLGVGLNAGDGPDVLLLPIGLSLGRRFLLEDSKISFVPYVNPVFAPAI
                     SGGDSQVDLAFGLGIDIRFSNAVDFRVSGGLGDIEGIALGIAVVR"
     gene            complement(9642..9902)
                     /gene="atpC"
                     /locus_tag="VFB89_15690"
     CDS             complement(9642..9902)
                     /gene="atpC"
                     /locus_tag="VFB89_15690"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF014838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HZM28791.1"
                     /translation="MRVTVISPEAAMFDGDADAVTAPAYDGEVGILPGHAPFMTLLGK
                     GRLRVRRGSSLSEFTVQGGFLQVVGDRVRVVAEHVQGAANVA"
     gene            complement(9902..11362)
                     /gene="atpD"
                     /locus_tag="VFB89_15695"
     CDS             complement(9902..11362)
                     /gene="atpD"
                     /locus_tag="VFB89_15695"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HZM28792.1"
                     /translation="MAATAAATQTRQAAGDKATQNVGRIVQIIGPVLDVEFEPEQLPE
                     IYNALLIEDKERGIRVVAEVQQHIGQNQVRTVAMSSTDGMVRGMEVVDLNGPISVPVG
                     AAPLGRILNVLGEPVDGGPPIPSDVERWPIHRETPKFTDLEPKTQIFETGIKVIDLIA
                     PFVKGGKIGLFGGAGVGKTVVIMELINNVAKGHGGKSVFSGVGERTREGNDLYLEMKE
                     SGVIKSCALVYGQMNEPPGARLRVGLSGLTVAEYFRDVEGQDVLLFIDNIFRFTQAGS
                     EVSALLGRMPSAVGYQPTLATEMGDLQERITSTTKGSITSVQAIYVPADDLTDPAPAT
                     AFAHLGATVVLSRAISELGIYPAVDPLDSASRILAPQFVGERHYNVAIGVQRTLQRYK
                     ALQDIIAILGMDELSEEDKQIVGRARRLQRFLSQPFSVATQFTGFEGKYVKLEETVAS
                     FERLLSGEFDQLPEQAFYMVGTIDEAVEKSKKLASA"
     gene            complement(11420..12316)
                     /gene="atpG"
                     /locus_tag="VFB89_15700"
     CDS             complement(11420..12316)
                     /gene="atpG"
                     /locus_tag="VFB89_15700"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HZM28793.1"
                     /translation="MAKSRQLRGRIRSVRNTRKITRTMELVSTSKLKRAQDRVIAARP
                     YAQALRDTLIDLLTPELAEQFPLLRPPKPIAQGGPNRAAVVLITSNRGLCGSFNANLI
                     KEARRRVEQVESAGYTADLYAVGKKGAGYFKYAGWKLASERHDIGDKPTPDHAVEVGA
                     RVIAAYETGQVASVDLVFARFQSALSTPPTIARILPIEVDAHRGAGRAAKTRPDYILA
                     PSAEAILAELLPLYVRNMIYQALVETAASEHGARRTAMKNATDNADELIKILTRTFNR
                     QRQAQITQEIAEIVGGAAALQG"
     gene            complement(12316..>13191)
                     /locus_tag="VFB89_15705"
     CDS             complement(12316..>13191)
                     /locus_tag="VFB89_15705"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292873.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HZM28794.1"
                     /translation="ASASDPAPLQYIAPYAGCAIAEYFMYEEGKATLCVYDDLSKQAT
                     AYRQMSLILRRPPGREAYPGDVFYLHSRLLERAAKISEDPNAIKQDPRIKKPGGSLTA
                     LPIIETQGGDVSAYIPTNVISITDGQIFLTTDLFYSNVRPAVDAGISVSRVGGNAQIK
                     AMKQVAGPLRLSLAQYRELEAFAQFGSDLDAATQKQLARGARLVEVLKQPQYQPMPVE
                     KQVAIIYAVTNGYLDDVQAPHIRQWEREFLEFLEADHGDILGAIRTKKTLDDSSTQGL
                     KAAITAFKALFNPAD"
BASE COUNT         2468 a         4298 c         4103 g         2322 t
ORIGIN      
        1 gtcactcaat ccctcacaaa tttcgggcgg ctgcggtcgc ctgctttgct gcctcaaata
       61 cgagcacgag ttttatgtca cggcccgcaa acgtttcccc aaggaaggca agagtctcca
      121 gacctcactc gggatcgaga aggtcatcgc agtcgacatc ttccgtgagc gggtctttct
      181 ccggagtgag gagcacggct cccgcatcat cccgctgatg cagttgcggg aagagatgga
      241 tcgcgccgtc gcgttgcttg cagttccgga cacccgcaca cgtcaggccc cgccaacctc
      301 cgagccggcg cccaccgtgc cgccagttca ggcgatcagt ccgacaccgg gcaacgggaa
      361 tgcgccggcc agcggcaacg ggcacgcaaa cggtgttcac ccgacgcgtc ggcgtcgccg
      421 ccggcgcggc ccgggcgata ccggcggctc gacaggcagt cccccacctg tcgagccctg
      481 agtgccgaag ttctacctca caaccgccat cgactattcc aacggcgagc cccatctcgg
      541 gcacgcccta gagaaaatcg gggccgactg cattgcccgg tatcggcggc ttcggggtga
      601 cagcgtccac ttcgtcatgg ggatggacga aaatgcgcag aaggtcgctc aagccgcgga
      661 gcgcgcgggg gttccgactg cggagtgggt ccgttccctt gccgagcggt tcgatgcaac
      721 ctggcgccgc ctgctctgtt cgcacgacga ttggatccgc actaccgaac cccgacatca
      781 tcgtgcgacc attgcgctca tcgagcgaat cgccgagcga agcccggacg atctttactc
      841 cggactgtac gaggggctct actgcatcgg gtgtgaggag ttcaagcagg aagcccagat
      901 catcaacggc cactgcgaag agcatcccaa tctggagctg attcacacca aggagcagaa
      961 ccactttttc cgtctgagcc ggtatcggga tgaagttctg cgcctcatcc agtcgggcga
     1021 gctgtcggta gagcccacca tccgccgcaa tgaggtactg agggttctcg aggctgggct
     1081 gcaggatata tccatctcgc gtcagcggct gtcatggggc attccgtttc cgggggactc
     1141 cagtcagacg gtgtacgtct ggttcgacgc gctgatcaat tacctctcgg cgacagggtt
     1201 tccggaagcg ggctacgagc ggctctggcc cgccgacctc catgtgatcg gcaaggggat
     1261 cagccggttt cactgcatca tctggccggc catgctgctc agcgccggag tggcgctgcc
     1321 ccggaaggtt tgggcccacg gttatgtgca gtggtccgga gccaaagtct ccaagtcggc
     1381 ggggaccgcg gtcagcctcg atgaggcgat cgaccgtcac ggaccagacg cgctgcggta
     1441 tttcctgctc cgtgaggtgg ggttcgaagc ggacgggaac ttcacatggg agcggttcga
     1501 cgagcgctac acggcggacc tggccgatgg cctgggtaac ctggcttccc gaaccttggc
     1561 gatgctgggt cgctaccgcg atggcaaggt gcccgatgcc gctccggcca cacgccttca
     1621 cgacgccgga tcgaggtgcc tcagccgcta cgccgcggcg atggatcgaa acgatctcag
     1681 gggtggggcc gaggctgcct gggagctggt ctccgaggcc aacctttaca tccagcagac
     1741 ggccccatgg acgctggcca aggagggccg tgaggcggag ctggatgcgg cgttagcagc
     1801 cctggcggcc tgcctttacc gattggccgt cctggtgacg ccattcgtac ccggcaaggc
     1861 gcaaacactg tggcaggccc tcggccagcg aggcgattcc accaccgcgc agtggacttc
     1921 actagaggcg cctcgcgtgg ctggcacgct taccacgaag cctgaggtgc tgttccccaa
     1981 gccaactccc acctgagact tacctgacga tgggcaaagt gttgcagcgt caaacggtta
     2041 gccgatctcg gatttgcctt gacaagggtt cctccgggca caactttagc ctacttttac
     2101 ggccggattc caacccggcc ggtcagtagt tctggcaggt ctcagcaagt cgaaggtacc
     2161 tggcccacag cgggctgggg cgcgaaccgt tagcgtttca gcctgacggg gttagttcgt
     2221 gctcgagggc ggcatttcag gagccgagat cggataatgg ccaaacaccg ctggaccatt
     2281 gtgctcgtcc ctcacggggc ggagccgtcc aaggtgctcg agctttcgtg gggcgcggtc
     2341 aagctggccg gcgcactcgc ggtggcgttg gtcgtggtcg ccctgctggt gggcaccgca
     2401 accgtctctc gtcgggtcga cttgagccgc acagcccggc tggaagacga aaatcagcgc
     2461 ttggctctgg agctgggcga gctacacggg cgtcttacca ccctgtccga cacgattgcc
     2521 cggatttctc agcgcgacgc caaaatccgt ctcctggcca acctcgagcc catcagcccc
     2581 caggtccagg cggcgggtat cggcggacca gcgctctcga cctccctacc cgcacacgtc
     2641 tccagcgcac tacttcggaa gacagccgac gttcgcctgg acgtgaacgc gttgatccgg
     2701 cgggcgaatc tgctcgcctc gtccttcagc gcggccgccg atagcctcgc ctcacatacc
     2761 gaccggatgt ccgccacccc gtcgatcatg cctacgcagg gttggctctc gagcgcattt
     2821 tccgcgatgc gggagcaccc gatccttcac gttgcccgtc cgcacgaggg aatcgacgtc
     2881 tcggccccaa tggggactcc gatcgaagca ccggccaatg gcgtcgtgcg ctcgaccggg
     2941 tgggaagcgg gatatggtaa cgtggtggaa atcgatcacg gcttcggcat cgtcactcgt
     3001 ttcgcccaca cgtcacgcat cctggtacgc ccgggtcagc gggtccagcg gggagatcga
     3061 atcgccctgg tggggaactc cggactcgcc accggccctc acctgcatta cgaagtgcac
     3121 gtgaatgggc gcgccgtaga tccgctgcgc tacgtcatgc ctgaaagcgt cgtcaccgac
     3181 tagaattgca ccttcggcgc ctcctgcgcg cccgcctgcg ccgcaaagta ctgccgcggc
     3241 ttgtccaagc cgaaaatctt cgccgtaagg ttgtagggga aacgccgaat gaacgcgttg
     3301 tactcgcctg ccgccgtgtt gtagtcggtc cgagccaccg aaatacgatt ctcggttccc
     3361 tccagctgat cctgcagctg cctgaagttc tcgctcgagc gaagctgggg atagttctcg
     3421 gagatcgcga gcagccgacc cagaccagcg cttagttgat cgttggctgc agccatgtcc
     3481 tgcacgtttc ctgcctgcac cgctcccgac aaacgcgcgc gcgcgttggc gatcgaaatg
     3541 aagaccgtat cctcttgctt ggtgacccct ttgacggtgt tcaccaggtt cgggatgaga
     3601 tctgcccgac gctgcagctg ggtctgaatc tggccctgag cctggttgac ccgctcatcc
     3661 atgctctgca tccggttata tccgcatcct gtcagcagca aggcggcgcc gagcagggtg
     3721 gcgggggacc accatcggct acgcatcatg agtgcagatc tcctagctcg agttgatcag
     3781 tgaattgggc ggctctttcg acggcacgga tgtacgcctc gaaatcgctc gctgcgagtg
     3841 tcggttttgc tgcagtgcga caagccagca cccggagcag cggtgccggt tcaaaaccca
     3901 gcagggtggc ggcatcctcg atgacccgcg acggttctgc cggagccggc cggcccgtga
     3961 ggctcaacag gcagcggaag aacagcacga tcgacgcgat cgtcccgccc gcccaggaac
     4021 ccagattctc ctcgccgtgg gcgacatacc gctgcctgag ccggagcagt ttgccccgaa
     4081 actccttctc gagcgcccgc cggagatcgg ctcgaccggg ctcgaccgga gcgacgaggt
     4141 ccgggcctcg caacacctgg tgggcggctc ggaggtcgca gatctcgatg gggaacacat
     4201 cgacgccctc gagccactct tcccgtgaaa tgaccagcgg aagaccggcc gtggccttgc
     4261 gccaacgagc gaatgccggc cggagccgcg ccaggtcttc gggctcggct cgttcgacga
     4321 tgagcatcag attcacgtcg gatgcgccgg agacgtagtc gccgcgggcg gcggatccga
     4381 agagaatcgc actgtacgtg gttccgatcg ccgcgtcgac gtcggccagg aatgggtcga
     4441 ccaggcggcg tatcccatca ttcatcagaa acgtcctccc gcgccgccac caccgccgcc
     4501 gccgccgccc ccaaagccgc caaatccgcc accaccgaag ccacccccac ccccacccca
     4561 accacctcca ccgccccaac cgccgccgat ccagggtccg ccaccccaat atatccgtcg
     4621 acggcggccg ccaaacctgc tggaaaggaa gaaaaagaaa agcatcagga ccaacggaag
     4681 ccagaatggg atgccgctgt tgccgccacg cgactcatgg ggcgatgccg cggccaaggt
     4741 tgagtcggtg acaccgtatc cgcgagccac caggcctacc agcgcccgta cacctgcgtc
     4801 gattgcctgg ctgtagttcc cttcgcgtgc agccggcagc atgagatcgc gcacgcggcc
     4861 ggcagcagca tcggtgacga tgccttccaa tccatatccg acctcgattc ggatcgcacc
     4921 ggagccgggc ttgccttcct gccggggaac gaagagcagc acaagaccgg cgttccgggt
     4981 agagtcgccg atctcggctt ttcgtcccac gccccaggag cgaagaatgg cgagggcgac
     5041 ttcagactcg cttcgatctc ccagagtcgg cagcgccacg accgcaatct ccgccccggt
     5101 ggcctggcga aggcgcgaga tcagagactc caactgcgct ttctcggctg gatccacgat
     5161 gccggcgacg tcggtcacgt agccggttgg gcgagcggga aacaggcggc tgacggcgtc
     5221 ctgctgcgca cccgccgctg acgcggcgag ttgaagggcc atcgcggcca cgatagcttt
     5281 gtgccgtgtc acgcctctgg tacgtcctcg cattggccgt cgttgcctgc gagcggccaa
     5341 acgatgttgc ggttcgcgtc tcgatccccg gccccgattc ggtcagcgcc cccgttccgg
     5401 gcgcgggact gatcgcgctt ccgtacgatc gcgacagcgt gctccgcgcg cttgcgacag
     5461 cggctcgcag cccgcgacct tcgaccaaag agctcgacag cctgtttcag caattcagaa
     5521 tcccgttttc caattacgcc aaggcgggat acgaagccga gcagttgcgc aggagccagg
     5581 actcgctgaa atcccggctc gactccctgc ctcgcaatgc cccggagtat cgctcgctgt
     5641 atgcccggtt cggcgagcaa agcagcgcct tgtccgctgc cgagaaacgg gagaaatccg
     5701 cacgcaccgc cctcgacgcc gcccgggcaa cattcgtagc ccggagcgac accctgcggg
     5761 cccgggtcag acgctgggaa gatagcacct accaaggata cgacagcatc gtccgcgagc
     5821 tctcgcgcgc gcgcggaatg accgcggtga ccgatacgac gggcgcggac ggatgggctc
     5881 atttccattt gcagagggga ccttggtgga tctacgctcg gtcctgggac gccagcgatg
     5941 ccaatgctga gtggtactgg aacgtcaagg tgacgggcga cactgtgctt ctcgactccc
     6001 acagcggcac tcggcgaccg aaatactgac tatgcgtacg tcatttctcc cacgaatggg
     6061 tccgtccagc cgtgcgctgg ctctgctgac gctggtctcg agcgtgctgc ccgcgatact
     6121 cgccgcgcaa ggctccagcc agaaccttct cgccgagcgc tccaaaggct ctgcgacagc
     6181 gccgatcacc gtctacgaga tgtcggactt ccagtgccct tattgccgtc agcacgcagt
     6241 agaaacattc cctgaattgg agaagagtta catccagacc gggaaagtac ggtgggtgtt
     6301 tctcaatttc ccgcttacct ccattcacca caacgcagca gcagcagcag agctcgcatt
     6361 gtgcggagcc cggcagggaa agttctggcc tttgcatgat ctgctcttca agtatcagca
     6421 agcctgggcg cccctcaaag agcccgggcc cttccttgtg acgctgatag actcggtcaa
     6481 cgctccgagg gcgccgatgg aggcatgcct caccgccggc gcagccaggc cggagctcga
     6541 ggccgacgct caggcggctc agcgttcggg cgcgcaaagc acgccgacct tttacatcga
     6601 gggcggactg ctggtgggag cgcagccggt ggcgttatgg aaggagattc tggattcggt
     6661 ctaccaggcg aagaaaagcg tgaacggcga agaaaagcgt aaccggtaaa cgggcctctt
     6721 catcgagctc ccgttcaccg tttaccgttt accgttcacg cagttcttat atcgtaacca
     6781 gcacatcgta caacgcgtgg gtccacgctg tgatcccgaa tccgcgggtc aggtagagtc
     6841 cactgaacag cacccccgcc acggcacgga aggcgaacga cccgagccga agctcgtcgc
     6901 cgtagggtcc tacataatgg aagagcgaga aaatcagagc gcccgtcata gtggcgaaca
     6961 ggcccgccgc aaccggtccc cagccgaata gtcgtttcgc cagccaggca agcgcgccaa
     7021 cgatgaggac ccgaaaaagc agctcctcgt aaatccctgc gccgagcgac accatgagct
     7081 gggtgggaag atcgagggcg actgccggcg caatcgcgag tcgcaggccg cccagcagcc
     7141 agcccgtcag tatgccggtg actatcccga acagcccggc gtacacgccc gcctcgatgg
     7201 ccatgagcag aaaaagctgt gggcgaagct tcccgccgga gcgatcccgt cggatcagca
     7261 ccgctcctcc gccgagcaga aacaatccga agacgatcaa cccgatgcgt ccgcccagcc
     7321 acacgaagac gctcttgagc aggacgtcgg cgccgttccg aaccccggtc agggagtcgt
     7381 gggacagtac gaacgcgagc gcctcgtaaa ggaggagcag cgggagggcg aacaggaggc
     7441 tgtatcgagg tgagcgggag aggcgccagt aggcggacat gggttcaagg gtaaacgatg
     7501 ccgttgaaaa agcgtgaacg gtgaacagta aacagtgaac gggggattcg gcgcagggaa
     7561 gcccacagtt ggtcggagca gtcctgttta ccgtttaccg ttcaccgttc acgcagttcc
     7621 aaaaacgcgg atcgggccgc ccgacatctg tcaggcagcc cgatcggttg gcatcgcagc
     7681 tcgatacgac gccggctagc tcagatcacc atccggcgat cgcttgccca agtcgagcta
     7741 cgaatgcaac tctcactaga caatggatca cctccttcca cagggtggca catagggcgg
     7801 acagccgaac actaagaact tcggcccagg acgacaagag gcgctattgc gtcttgaccg
     7861 gggcggtctg ggtcacgacg ccttcgtaga ccaccgatcg cggcacaatg acgccggccg
     7921 tgataatggt aaacaacacg tccgtgaagc tcgcgtgcgt cttgatcttc agatcggcga
     7981 tacctttgcc gcctgcgagc tggctcgcga ggctcttgtt cagtgcggga cgcttgaggc
     8041 ccattattcc ccagaaggcc caaatcgcgt gcgagctgac ccgaaagctg tcgaccggag
     8101 cggcctgtcc ggcaggtgac gccatggtta ccggcacgcc cagactggtc gcatcgaagt
     8161 tgaggctaca cccgccaaga atgatgggcg tcagggccag catgacgcgc gcgagccgag
     8221 cgctcaaaga ccgggaatcc tgaaggcgta gtgcgcaccc accgagaaaa tgcccggatc
     8281 gtccccgttc acgaacaccc ggtcgtacgc tgtttgcagc ccaaagccgt tgaggaaatt
     8341 gaactcgatg cccgcgctca tcccgaaatt cgaatcggtg tccgaaggtc catcgttgac
     8401 agaggtttcg gtgatatcga tgcggggagc gatccacggc tttatcgcca gcgccggatt
     8461 ggggatggtg agcgcgaggc caagcccgat gggaaagtgc caagtcttca cgcccgtcga
     8521 atcggggccc accctcccat agccgacgcc gccttgcacg gtcgccgaga acggcaccag
     8581 aggcccaccg atcagcttgt agttgccggt ggcaccccac gtcgtgagcg tgttgtccgc
     8641 accgctggga ttgaacgacg cgatggttgc cgtgacgccg agcttgctcc aaccgagccg
     8701 gccactgagc cccagggtgt agccatcacc ggctgcgccg tcgggaaatg caacgtcgcc
     8761 gtagataccc agaccggtag gaacgccgct gttatagacg ggaagaccga gtgcttgggc
     8821 cgaaccgatt gacgcagcgc cgagcgtcag cgcgagggcg aggccgagcg cgcggtggga
     8881 cggtctcatc agcttcctcc ctggtgtccg gcacgagccg gccgcggagc cggcaacagg
     8941 cgggcgggaa gctagtcgcc aatcgcgcgg gaagtcaatg aggtgatagc ccttagccgc
     9001 tagctgatag ctgatagctg atggtcaaca cctcctacct caccaccgca atgcccagcg
     9061 cgatgccttc gatgtcgccg agacccccgc tgacgcggaa atcaaccgcg ttgctgaacc
     9121 ggatatcgat gcctagtccg aacgccaggt ccacctggct gtctccgccc gagatcgcgg
     9181 gagcaaacac cgggttcaca tagggaacga agctgatctt tgaatcttcc agcaggaatc
     9241 gccgaccaag cgacaagccg atcggcaaca acaggacatc cggcccgtcg cccgcgttga
     9301 gtcccacgcc gagggtcagc gccccgtcga agggaaactg ctcgctagct ttcagaaccc
     9361 tggtacggaa gtccccaccc agcagaatcc gcgtgtcgcc ggaaccctcc agatcttcga
     9421 agccgcctcg gatcccgatg tcataggcgc cgtgtccaaa gccgtagaag ccttcgaccg
     9481 ccaggttcac atctccactc gggaacgaga ggttgccgcc aaattcatgg ctggtgaagg
     9541 cccggtaggg agccttgaac acgggcgttg cggtttcctg tgctgcaagc gttcccgtgg
     9601 cagccgacat gagcgccagt gccgcgaaac caatccgcgc gctacgccac attcgccgct
     9661 ccttgaacgt gctcggccac cacgcggacg cgatcgccga ccacctggag aaagccaccc
     9721 tgaaccgtga attcgctgag agacgagccg cgccgcactc gcaggcggcc tttgccgaga
     9781 agcgtcatga acggcgcgtg cccggggaga atgccgactt caccatcgta cgcgggcgcc
     9841 gtcacggcgt cggcatcgcc gtcgaacata gctgcctcgg gcgagatgac tgtgactcgc
     9901 atcacgcgct cgcgagcttc ttcgacttct ccactgcttc gtcgatggtg ccgaccatgt
     9961 aaaacgcttg ttcgggcagt tggtcgaatt cacccgacaa cagccgctcg aagctcgcca
    10021 ccgtttcttc cagcttcacg tactttccct cgaagccggt aaactgggtc gcgactgaga
    10081 acggctgtga gaggaagcgc tgcagtcgcc gcgcgcgtcc cacgatctgc ttgtcctcct
    10141 cagacagctc gtccatgcca agaatggcaa tgatgtcctg cagcgccttg taccgctgca
    10201 gcgtccgctg cacgccgatc gcgacgttgt agtgccgctc gccgacaaac tggggagcca
    10261 gaatacggct cgcgctgtcc agcggatcca ccgccggata aatgcccagc tccgagatgg
    10321 cgcgggagag caccacggtc gcaccgaggt gggcgaatgc cgtcgccggc gccgggtcgg
    10381 taaggtcgtc agccggaacg tagatcgcct gcaccgacgt gatcgagccc ttggtggtcg
    10441 aggtgattcg ctcctgcagg tcgcccatct cggtcgccag cgtcggctga tagcccaccg
    10501 cgctgggcat ccggccgagc agcgcggaaa cttccgagcc ggcctgggtg aaccggaata
    10561 tgttgtcgat gaagagcagc acatcctggc cttcgacatc gcggaagtac tccgccaccg
    10621 taaggccgct taggccaacc cggagccgcg ctcccggcgg ctcgttcatt tggccataca
    10681 cgagggcgca ggacttaatg acgccgctct ccttcatttc caagtagaga tcgttcccct
    10741 cgcgggtacg ctcgcccacg cccgagaaca ccgacttacc accatgcccc ttggcgacgt
    10801 tattgatgag ctccatgatg acgacagtct tcccgacgcc cgcgccgccg aacaacccga
    10861 tcttgccacc cttcacgaac ggcgcgatca gatcgataac cttgatgccg gtctcgaaga
    10921 tctgggtctt gggctcgaga tcggtgaact tgggcgtctc tcggtggatc ggccaccgct
    10981 ccacgtccga cgggattggc ggaccgccgt ccaccggctc accgagaacg ttcagtatcc
    11041 gacccaacgg cgcggcaccg accggtacgg agatgggtcc attcaggtcc acgacctcca
    11101 ttccgcggac catgccgtcg gtgctgctca tcgcgacggt ccgtacctgg ttctgaccga
    11161 tgtgctgttg cacttcggcc accacgcgga tgccgcgttc cttatcttcg atcagcagag
    11221 cgttgtagat ctcgggcaac tgctcgggct cgaactcgac gtcgagcacc ggaccgatga
    11281 tctggacgat gcggcccacg ttctgggtcg ccttgtcacc ggcggcttgc ctcgtctggg
    11341 tggctgcggc tgttgctgcc atggtctatt ccatcagtca gtggttgttc gtccggggtc
    11401 gggagcccgc tcccgcgctt cagccctgca acgccgccgc gccacccacg atttccgcga
    11461 tttcctgcgt gatctgggcc tggcgttggc ggttgaatgt ccgggtcagg attttgatca
    11521 gctcatcggc attgtcagta gcgtttttca tcgccgtgcg ccgggcgccg tgctcggacg
    11581 ccgcagtctc gaccagcgcc tggtagatca tgttgcgcac gtagagcggc agcagctcgg
    11641 cgagaatggc ttcggcgctc ggagcgagga tgtagtccgg ccgggtcttc gccgcgcggc
    11701 cggcgccccg atgggcgtcg acctcgatcg gcagaatgcg tgcaatggtc ggcggggtgg
    11761 acagcgcgct ctgaaagcgg gcgaacacca ggtcaacgct ggcaacttga ccggtctcgt
    11821 acgcggcaat gactcgagcc ccaacctcca ccgcatggtc aggggtcggc ttgtcaccga
    11881 tgtcatgacg ctcgctggcg agcttccaac ccgcgtactt gaagtacccg gctcccttct
    11941 tgcccaccgc atagagatcc gcggtgtacc cggccgactc gacctgctcg acgcggcgac
    12001 gcgcctcctt gatcaggttg gcgttgaagc tgccgcagag acctcggttc gaggtaatca
    12061 agacgacggc tgctcggttc ggcccaccct gcgcgatcgg cttgggcggg cgaagcagcg
    12121 gaaactgctc cgcgagctct ggagtgagca agtcgatcag ggtatcgcgg agggcctggg
    12181 cgtaaggcct ggccgcgatc acgcggtcct gcgcgcgctt gagcttggac gtggagacca
    12241 gctccattgt ccgcgtgatc ttgcgcgtgt tgcggacgga gcgaatccgg ccgcgaagtt
    12301 gccggctctt cgccatcagt cagccgggtt gaacagcgcc ttgaacgctg taatcgcggc
    12361 cttgaggccc tgggtgctgg aatcgtccag cgttttcttg gtgcgaatcg cccccaggat
    12421 atcgccgtgg tcggcctcga gaaactcgag gaattcccgt tcccactggc ggatgtgcgg
    12481 tgcctgcacg tcgtcgagat agccgttcgt caccgcatag atgatggcca cttgcttctc
    12541 taccggcatc ggctggtact gcggctgctt cagcacctcg accagccgag caccgcgggc
    12601 gagctgcttc tgggtagcgg catccagatc ggagccgaac tgggcgaacg cctccagctc
    12661 acggtactgg gccagcgaga gacggagcgg gcccgcgacc tgcttcatcg ctttgatctg
    12721 tgcgttccca ccgactcggc tcacactgat gccggcatcc actgcgggac ggacgttgga
    12781 gtagaacaga tccgtcgtaa ggaagatctg gccgtccgtg atcgagatga cgttggtcgg
    12841 gatgtacgcc gaaacgtcgc cgccctgggt ctcgatgatc gggagggcgg tgagcgatcc
    12901 ccccggcttc ttgatgcggg gatcctgctt gatcgcgttc ggatcctcgg agatcttggc
    12961 ggcccgctcg agcaggcggc tgtggaggta gaacacgtcg cccggatacg cctcgcggcc
    13021 cggcgggcga cgcagaatca gcgacatctg gcggtaggcg gtcgcctgct tggaaaggtc
    13081 gtcgtacacg cagagggtcg cctttccctc ctcgtacatg aagtactcag cgatcgcgca
    13141 gcccgcgtag ggcgcgatgt actggagcgg cgccgggtcc gacgcggagg c
//
LOCUS       DASRJQ010000695         4502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_569879, whole genome shotgun sequence.
ACCESSION   DASRJQ010000695 DASRJQ010000000
VERSION     DASRJQ010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569879"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VFB89_15710"
     CDS             complement(<1..740)
                     /locus_tag="VFB89_15710"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZM28795.1"
                     /translation="MAAAPVSAPLLRRYRDEFPIFRNAIYLNSCSLGPLSRRSRLRVN
                     QYLDQWESRGAAAWYDTWWSALSELRDRYGRMIGASGSAIALHPNISTALTSVAETLD
                     YRQRRRVVITRLDFPTVAYQWLARAGEGVELVMLDTPDGVQVPLEAFERAVDDRTALV
                     ATSHVFFTSGAIQDVRALADIAHRRGALLLIDGYHAAGQVPVDVGVLGPDFYCAGGLK
                     WLLGGSGIAFLYVRPELTGTLQTRATGW"
     gene            784..1044
                     /locus_tag="VFB89_15715"
     CDS             784..1044
                     /locus_tag="VFB89_15715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28796.1"
                     /translation="MRDSWLRLTARLTGRAIVNPRLALDLLRTGWAFRRREWWRKPPF
                     LPLPDRTYIRWRMYTAYADENAVPPVEDVVRFARWRRETMGL"
     gene            1083..2372
                     /locus_tag="VFB89_15720"
     CDS             1083..2372
                     /locus_tag="VFB89_15720"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZM28797.1"
                     /translation="MPHVVIIGGGFAGIYAVRALKRAAVRVTIIDRRNYHLFQPMLYQ
                     VATAGLNPSDITAPIRSVVRRQRNVEVVLGEVCGIDKAERRVELTDGSSFTYDYLLVC
                     TGARHSYFGHDDWERLAPGLKGLEDALEIRRRILKAFEKAEREPDPIKRQALLTFVIV
                     GGGPTGVEMAGAVAEIRSYALRRDFRHIDPREATIVLLEGTQRLLNAYPDSLSEEAKR
                     ELRSLGVVVRTETFVTNVRPGAVEAAGWTIPTHTVIWAAGNTASPLLKTLDVPLDRQG
                     RVIVGRDCTIPDHPEVFVLGDAALFQENGHAVPAISPAAIQMGQYAARTIQRDLAGKT
                     REPFAYWDKGQLAVIGRGQAVADLGRLRFGGFIAWLLWIFVHIFFLIGFRNRLLVLIE
                     WAWAYFTYQRGARLITEPWVPEAEAERVPAGSGVTES"
     gene            2312..3325
                     /gene="queG"
                     /locus_tag="VFB89_15725"
     CDS             2312..3325
                     /gene="queG"
                     /locus_tag="VFB89_15725"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HZM28798.1"
                     /translation="MGSRSRGRAGSRRQRSDRVLTALARTGTLEATEIKAEAERLGFV
                     ACGITDLSPTPHGAELDEWLARGRAGTMRYLHRQARKRKDPQRIDPDARSIVVVLDNY
                     FTPDQAGDRDPPRVAKYARGTDYHRVTLQRLEQLADFLRQRGARVARAYADAGPVPER
                     ELAQRAGLGWIGKNTMLIRPGLGSFFFIGSVLTDLALPIDPPFALDQCGRCTQCLDAC
                     PTEAILSPRLLDATRCISYLTIEHRGPIPDEMVPKLDGWAFGCDICNDVCPWNQRFAT
                     ETEIEGLRPRGLLHGVEANYFDRMTEEDFSQGFGDTALSRPGLSGMRRNLRSAFASRA
                     DHP"
     gene            3322..3873
                     /locus_tag="VFB89_15730"
     CDS             3322..3873
                     /locus_tag="VFB89_15730"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZM28799.1"
                     /translation="MTAAIRLARSEDAAAIAAFSARTFFETYAESNDPADMTAHLAAK
                     YGEAIQGAELADPRLVYLLAEIDGALAGFALLHLDHAPDNTTLSRPVELVRFYVAKEW
                     HGHGVAQTLMSACRDQAIRRGGRTLWLAVWQSNARAIAFYHKMGFAVAGTIAFRLGSR
                     LQTDHMMTLPLESVDPVEWVGRG"
     gene            3870..4394
                     /locus_tag="VFB89_15735"
     CDS             3870..4394
                     /locus_tag="VFB89_15735"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HZM28800.1"
                     /translation="MTRPAISSPDKGSVEPRLAPAERRRYQRLETLANLLDNAIPIPG
                     TSWRIGLDPLLGLAPGVGDALGAAASAWILIEAVRFGVSRAVLLRMLYNIVIDTVLGT
                     IPGAGDLFDFVWKSDSKNMSLLRRHLAEPGEAQRASRWLLVGVLLALALVAVGSVVAL
                     VWLVKYLVTLSPLF"
BASE COUNT          796 a         1433 c         1431 g          842 t
ORIGIN      
        1 aaccatccgg tcgcacgggt ctggagtgtc ccggtgagct cgggtcgcac gtagagaaaa
       61 gcaattccgg atccacccag gagccacttg agcccgcccg cacaatagaa atcggggccg
      121 agcacgccaa cgtccaccgg cacctgaccc gcggcgtggt agccgtcgat cagcagcaga
      181 gccccccgtc ggtgggcgat gtctgccagg gctctaacat cctgaattgc gccggaggta
      241 aagaacacgt ggctggtcgc caccagcgcc gtacggtcgt ccacggctcg ctcgaacgct
      301 tcaagtggaa cctgtacccc gtccggcgtg tccagcatga cgagctcgac tccttccccg
      361 gcgcgcgcga gccactggta cgctaccgtg gggaaatcga gtcgcgttat gacgactctc
      421 cggcgttgtc tgtagtcgag cgtttccgcg accgacgtga gcgctgtcga gatgttcggg
      481 tggagcgcga tggcagaacc ggaggcgccg atcatccggc catagcgatc gcgcagctcg
      541 ctcagagcag accaccatgt gtcgtaccac gcggccgcac cccgcgactc ccattggtcg
      601 agatactggt tgacccggag ccgcgagcga cgcgacagcg ggccgagaga gcagctgttg
      661 agatagatcg cgttgcggaa aatcgggaac tcgtcccgat accgccgcag aagcggcgcc
      721 gaaaccggtg cggcggccat agggagggga atatacccca gcagcaggaa ttatcttgca
      781 acgatgcggg attcctggct tcgactgact gctcggctca cggggcgggc catcgtcaat
      841 ccgcggctcg cgttggacct ccttcgcaca ggctgggcct tccgtcggcg cgagtggtgg
      901 cggaagccgc cttttcttcc cctgcccgac cgcacctata tccgctggcg catgtacacg
      961 gcctacgccg atgagaacgc cgttcccccg gtcgaggacg tagtcaggtt tgctcgctgg
     1021 cggcgtgaga caatgggact atgactgccc gcaatccctc gaatcgggat accgatcgcc
     1081 gcgtgcccca cgtcgtcata atcggcggtg gatttgccgg catctatgcg gtccgcgcgc
     1141 taaaacgcgc tgccgtgcgg gtgacgatca tcgatcgccg gaactaccac ctgttccagc
     1201 cgatgctgta tcaggtggca actgccgggc tcaacccctc cgacatcacg gcaccgatcc
     1261 ggtctgtcgt tcggcggcag cgaaatgtcg aagttgtttt gggtgaggtc tgcggaatcg
     1321 acaaggcgga gcgtcgggtc gaactgaccg atggaagcag cttcacatat gactacctgc
     1381 tcgtctgtac gggagcccgg cacagctact tcggacacga tgactgggaa cgcctggcac
     1441 ccggcctgaa gggattggaa gatgcgctcg agattcggcg ccgaatccta aaggccttcg
     1501 agaaggccga acgtgagcca gacccgatca agcggcaggc gttgctcacg tttgtcatcg
     1561 tgggcggagg gccgactggg gtcgagatgg ccggcgccgt ggccgaaatc agaagttacg
     1621 cccttcgccg cgactttcga cacatcgatc cgcgtgaggc aacgattgtc ctgctcgagg
     1681 ggacgcagcg cttgctgaat gcttacccgg actccttgag tgaagaagcc aaacgggaat
     1741 tgcgcagtct cggcgtggtg gtgcgcaccg aaaccttcgt caccaacgta cggccagggg
     1801 cggtggaggc ggcaggatgg acgatcccga cgcatacggt aatctgggcg gcgggcaaca
     1861 cggcgagccc actgctcaaa acactcgatg tgccgctgga tcggcagggg cgcgtcattg
     1921 tagggcgcga ttgcaccatc ccggatcacc ctgaagtgtt cgtgctgggc gacgccgctc
     1981 tgttccagga gaacggacac gcggttccag cgatttcccc tgccgccatt caaatgggcc
     2041 agtatgccgc acggaccatt caacgagatc ttgcggggaa aacacgcgag ccatttgcct
     2101 actgggacaa gggtcagctc gcggtaatcg gtcgagggca ggcggtcgct gacttaggtc
     2161 gtctgcgttt cggtggtttc atcgcgtggc tcctgtggat cttcgttcac attttctttt
     2221 tgatcggctt tcgcaatcgc cttctggtcc tgatcgagtg ggcctgggcg tatttcacct
     2281 accagcgggg cgcgcgactt attactgagc cgtgggttcc cgaagccgag gcagagcggg
     2341 ttcccgccgg cagcggagtg accgagtcct gacagcccta gcgcgcactg gcaccctcga
     2401 agccaccgag ataaaagcgg aggccgagcg cctcggcttc gtcgcctgcg gcatcaccga
     2461 cctgagcccc acgcctcatg gagccgagct ggatgagtgg ctggcgaggg gccgcgccgg
     2521 aaccatgcgg tacctgcacc ggcaagcccg gaagcgcaag gatccacagc gaatcgatcc
     2581 ggatgcgcgg tcgatcgtgg tggtgctcga caactacttt acgcccgatc aggcgggcga
     2641 ccgggatccg cctcgcgtag ccaagtacgc ccggggcacg gactaccacc gggtgaccct
     2701 gcagcgactg gagcagctgg ctgactttct ccggcagcgg ggtgctcgcg tcgcccgggc
     2761 ctacgcagac gcgggtccgg tccccgagcg cgagctggcc caacgcgccg gcctgggatg
     2821 gatcgggaag aacaccatgc tgatccgccc tggcctggga tcgttcttct tcatcggctc
     2881 ggtcctgacc gatctcgcgc ttccgatcga tccgcccttc gccctcgatc aatgcggtcg
     2941 ctgcacccag tgtctcgacg cgtgccccac cgaagcgatt ctgtcgccgc ggctgttgga
     3001 tgcgacccgc tgcatttcgt atctcaccat cgagcaccgc ggtcccattc cggacgagat
     3061 ggtacccaag ctggacgggt gggcattcgg atgcgacatc tgcaacgatg tgtgtccctg
     3121 gaatcaacgt ttcgccacgg aaaccgagat cgagggattg agaccgcgcg gcctgttgca
     3181 tggcgtcgag gcgaactatt tcgatcggat gacggaggag gatttctcgc agggcttcgg
     3241 agacacggcg ctgagccgcc ccggcctctc aggtatgcgg cgaaacctcc gctcggcatt
     3301 cgcgtcgcga gccgaccacc cgtgacggcc gccatccgtc tggcacggtc cgaagatgcc
     3361 gccgccatcg cggcattctc ggctcgcacc ttcttcgaga cgtatgcgga gtccaatgat
     3421 cccgcggaca tgaccgcgca tctcgccgcc aagtacggcg aagctattca gggcgccgag
     3481 ctcgcggatc cgcgcctggt gtacctgctg gccgagattg acggtgcact ggctggattt
     3541 gccctcttgc acctggacca cgcacctgac aacacgactt taagtcggcc agttgagctg
     3601 gtccgcttct acgttgcgaa ggaatggcac ggccacggcg tcgcccagac actgatgagc
     3661 gcttgcaggg accaagccat ccggcgtggc ggccggacct tatggctcgc cgtgtggcag
     3721 tcgaacgctc gtgccattgc cttctaccac aagatgggct tcgccgtcgc tggaaccatc
     3781 gccttccggc tcggctcacg gctgcaaacc gatcacatga tgactctgcc gctggagtcg
     3841 gtcgaccccg tcgaatgggt tggccgcggg tgactcgacc cgcgatttct tcgcctgaca
     3901 aggggtcggt cgagcccagg ctggcgccgg ctgaacgccg acggtatcag cgactcgaga
     3961 ccctcgccaa tctgctggac aatgcgattc cgattccagg aacttcgtgg aggatcggcc
     4021 ttgatcctct gctgggtctt gcccctggag ttggtgatgc cctcggcgcg gccgcatccg
     4081 cctggatatt gatcgaggcg gtgcgtttcg gagtctcgcg cgcggttctc ctccggatgc
     4141 tctacaacat cgtgatcgac accgtgcttg gcaccatccc tggcgcgggg gatctcttcg
     4201 actttgtgtg gaagtccgac agcaagaaca tgagcctgct taggcgtcac ctcgcggagc
     4261 ccggcgaagc ccaacgggcg agccgttggc ttctcgtcgg tgtgctgttg gcgctggccc
     4321 tggtggccgt gggctcggtt gtcgcgctcg tctggcttgt caagtacttg gttacgctct
     4381 cacccttgtt ctagaccgta tgcgattcga gaccttggcg gtgcacgccg gccatcagcc
     4441 cgatccggca accggcgctg taaccccacc catccaatta tcaaccacgt tcgagcgagc
     4501 ca
//
LOCUS       DASRJQ010000696         6760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_3235959, whole genome shotgun sequence.
ACCESSION   DASRJQ010000696 DASRJQ010000000
VERSION     DASRJQ010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6760
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3235959"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1227
                     /locus_tag="VFB89_15740"
     CDS             <1..1227
                     /locus_tag="VFB89_15740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28801.1"
                     /translation="PHKLTGVDGLRQVVEQLDGFEAPAAAWERTILPARVDRYDPALL
                     DTLCLTGRVAWGRTSGRNGNSENGSAPSTRTAGPIRTTPVALFMRETAQYWLPAEIAI
                     EDAALSEYGRRVREVLVRRGASFFHELVGGSGLLATQVEQALGELAALGMVTSDGFAG
                     LRALITPSAKRKPLIPAPRRNRTVPFGIESAGRWALLEGHPDECTDPQRIEHKARTLL
                     RRYGVMCRRLLEREANAPLWRDLLMAYRRLEARGEIRGGRFVAGLAGEQFALPEAVGQ
                     LRAIRRQESRERLVGLSGADPLNLTGIVTPGERIPALANNRVLYEDGVPVLARSGGET
                     RVLNAEVVDRVAKLAPALIRRGIGPALRAQLALSGVPASKATLERPPKRRAHRDANPT
                     SSAAVSEDVAATSDSR"
     gene            1264..3318
                     /locus_tag="VFB89_15745"
     CDS             1264..3318
                     /locus_tag="VFB89_15745"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZM28802.1"
                     /translation="MSAASCRSLTSRCSAGCLALFVSTQLAAQMPPVIPVESFFDSPQ
                     IAAAQISPDGRWLAYLKPYQGKLNVFVRPVGADTTKESRLTADTVRPIRQYYWSADGK
                     KVLYLQDKGGNENFHIFSVGVDAAAAPGDARDLTPYEGARAFVADVPRDLPNRIFIAL
                     NKRDPSAFDAYWLDLDSGKLELAAQNPGRFAGYLADRSHQIRVGLAQGPAGENEVHVR
                     SDSTRPWRLLVSYPATENVQPLRLNPDGRHLYMISNHGETDLNRLVSLDLDNGQEQVV
                     HEDPEHTAEPFANNEVLFGEGTDSVFWTAYNADTVRIYPRNEAIAADLARIRKLHNGT
                     PIVTSMTRDEQRWVVSFDAPTDPGATYLYDRTTGKGTFLFRPRPWLKPTELAEMKPIS
                     FKARDGLSIHGYLSVPNGIPARNLPMVLLVHGGPWARDDWGYQPEAQLLANRGYAVLQ
                     INYRGSTGYGKRFYNAAVKEFAGAMHNDLIDGVNWAVKQGLVDPKKMCVYGGSYGGYA
                     TLVSVTFTPKQFTCAVDYVGPSSLITLVESFPAYWRPFLEGSWYRFVGDPAKPEDRKD
                     MWARSPLSRVDQITTPLVIVQGANDPRVTKKEADQLAVALRQRGVKVQYLIAANEGHG
                     FLNPDNRLALYHTMERFFAQYLKGRTQAKTPAGVDARIRAMTVDVDTLKAGQGQDRAA
                     TR"
     gene            3391..6063
                     /locus_tag="VFB89_15750"
     CDS             3391..6063
                     /locus_tag="VFB89_15750"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZM28803.1"
                     /translation="MADPRAELEHALSGRYRLERELGRGGMATVWLAHDLHHDRPVAL
                     KVLDPGLEQSLGTERFQREIRLAARLQHPHILSVYDSGENAGQLWFTMPFVEGESLRD
                     RLRREGPLPVGQALRITREAAQALQYAHEHGVVHRDVKPENILLTRDGSTLVADFGIA
                     RPLEGPPGTQLTVAGLVMGTPSYMSPEQATGSTDLDGRSDIYSLACVLHEMLSGSPPF
                     KGSTIQSVLSKHITESPPPLTASNRALPVSIARAVTKALAKVPADRFTSAAEFAAALE
                     PSGVARAYRGRRPRSSVVAGVGGVILALAGWGLYAMRSRETSAQPRESRGTTLATGFN
                     RQLSQLTFGEGVEEWPAWSPDGKRLAYVAEAGGFKQLFLRTLANGGEQQVTHDPRDHI
                     LPNWSPDGKRLAFVRARADSGTLDPSDLDGWFYEGGDVWTIDLASDSSSKLIDGAFGP
                     AYSPDGSRLAVDADWAGPERIWVVDANGRNPRQITTDSSEAVIHAKPQWSPDGSKIAF
                     RRIEKTKSDIVIVDVATQAMTRVTDDNVVDLDPVWSRDGQRIYFSSYRGGSINLWWIP
                     VAPDGHPTAGPSQLTTGAGEDVEPAIGPDGQRIAFGVRGINSDIWRLPVSPETGRATG
                     DPSPVLETTRVESRGAWSPDGRVIAFNSDRLGEMNLWVRTLADGSERQLTRGTGGDYQ
                     PNWSPDGRRIAFFSARGGNTDIWAVDVADGKLTRLTDDRALDTNPFYSPDGKQIAFMS
                     DRTGRSEVWIMNADGSNQRRLTSVGTWGHFLRWTRDGRGIVFRSESAVPVQVYRMSVA
                     DGSLTKLPDVLSGGHMSFSPDQSLILDVQGHKTIWVYPVNGQAPYKIFSFPSPDVRID
                     YPVWSPDGRWVLFDHAAPRGGDLWMLELGARASE"
     gene            complement(6190..>6760)
                     /locus_tag="VFB89_15755"
     CDS             complement(6190..>6760)
                     /locus_tag="VFB89_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28804.1"
                     /translation="DLEARATALKSIDRQLNHFEELLAKWESAQAEAARGLEQTLARQ
                     GAVEALEAQVKHVFEMAERAVEHVQELGAARRQVEETRELLQDTQAQFKGVEEALNGF
                     ESRRRQLERAEQRLARAEALAVDVRATVESLQAQRTVVDHVIERAGALAFQMKQAEAL
                     VEVLRRERTLATDLKAAMESVQEEDEGEK"
BASE COUNT         1197 a         2173 c         2227 g         1163 t
ORIGIN      
        1 ccgcacaagc tgaccggcgt cgatggcctg cggcaggtcg tcgagcagct cgacggattc
       61 gaagcgcccg cggccgcgtg ggagcggacc attctcccgg cgcgtgtcga tcggtacgat
      121 ccggcgctgc tcgacacgct ctgcctgacc ggtcgcgtgg cgtggggccg gaccagcggc
      181 aggaacggaa actctgaaaa cgggagtgca ccaagtaccc ggaccgccgg cccgatccgc
      241 acgacaccgg tcgcgctgtt catgcgggag accgcccaat actggctgcc ggctgaaatc
      301 gcaatcgagg acgccgcatt gtccgagtac gggcgccggg tccgcgaggt gctggtccgg
      361 cgcggtgctt cgtttttcca tgagctggtg ggcgggtccg gattgcttgc gactcaggtc
      421 gagcaagcgc tcggcgagct ggctgctctt ggcatggtga cttcggacgg ctttgccgga
      481 cttcgagcgc tgattacgcc atcggcaaag cggaaaccgc tcatcccggc gccaagacgc
      541 aatcggaccg tgccgttcgg gatcgagagc gcgggccggt gggcgctcct ggaagggcac
      601 ccggacgaat gcaccgaccc ccaacggatc gagcacaagg ctcggacgct gctccgccgg
      661 tatggtgtca tgtgccgccg cctgctcgag cgggaggcca acgcacctct ctggcgtgac
      721 ctgctgatgg cgtataggag gctcgaggcg agaggcgaga tccggggagg ccgctttgtc
      781 gcgggtctgg cgggagagca gttcgcgttg cctgaggccg tgggccagct gcgcgcgatc
      841 cgccgacagg aaagtcgcga gcgtctcgtg ggattgagcg gtgccgatcc gctcaacctt
      901 accggcatcg tgaccccggg cgagcggatc cccgccctgg ccaataaccg cgttctgtat
      961 gaggacgggg tccccgttct ggcccggagt gggggggaga ccagggtgct caatgccgaa
     1021 gtcgtggacc gggtggcgaa actggcgccg gccttgatcc gtcgggggat tggcccggct
     1081 ctccgggctc agctcgcgct gagcggggtg ccggcctcca aagcgaccct cgagcgtccc
     1141 ccgaaacgcc gggctcaccg ggatgcaaac ccgacgtcat ccgccgctgt ctcagaggac
     1201 gttgccgcga catcggattc gcgctaggcg gtcacgccaa cgtcacccac atcggagcac
     1261 cttatgtcag ccgcgtcgtg ccgtagtctc accagccggt gcagcgccgg ttgtcttgcc
     1321 cttttcgtct ctacgcaact ggcggcccag atgccgccgg tcattccggt cgaaagcttc
     1381 ttcgatagtc cgcagatagc cgcggcgcag atctcgcccg atggccgctg gctcgcgtat
     1441 ctcaagccat accaaggcaa gctcaacgtt ttcgtccgac cggtaggggc cgatacgact
     1501 aaggaaagcc ggctcaccgc ggacacggtg cggcccatcc ggcagtatta ctggtcggcc
     1561 gacggcaaga aggtgctgta cctgcaggac aagggcggca atgagaattt tcacatcttc
     1621 tcggtcggag tcgacgcggc cgcggccccc ggcgacgctc gcgatctcac tccctacgag
     1681 ggcgcccgtg cgttcgtcgc ggacgtgccc cgggatctgc ccaaccggat attcatcgcc
     1741 ctgaacaagc gcgatccgag tgcgttcgac gcgtactggc tcgatctcga cagcggcaag
     1801 ctggagctgg cggcgcagaa tccgggacgg ttcgccgggt atctggccga tcggtcgcat
     1861 cagatccgcg ttggactggc acagggtccg gcaggtgaga atgaggtcca cgtgcggtcc
     1921 gactcgacca gaccctggcg gcttctggtc agctatccgg cgacggagaa cgtgcagccg
     1981 ctccgcctga acccggacgg acggcacctc tacatgatct cgaatcacgg cgagaccgat
     2041 ctgaatcgcc tggtctcgct cgacctggat aacggccagg agcaggtggt ccacgaggat
     2101 ccggagcata cggccgagcc gttcgccaat aacgaggttt tgttcggcga gggcaccgac
     2161 tcggtcttct ggaccgcgta caacgccgac accgtgcgga tctatccccg caacgaggcc
     2221 atcgccgcgg atctggccag gattcggaag cttcacaacg gaacacccat cgtgacatcg
     2281 atgactcgcg acgagcagcg ctgggtggtg agcttcgatg ccccgacgga tccgggcgcc
     2341 acatacctct acgatcgcac gaccggcaag ggaacgttcc tattccggcc ccggccctgg
     2401 ctcaagccca ccgagctggc ggagatgaag ccgatcagct tcaaggcccg tgacggactc
     2461 agtatccacg ggtatctgag cgtgcccaat gggattcctg cccgcaatct gcccatggtc
     2521 ctgctggtgc atggcggccc ctgggcacgg gacgattggg gataccagcc ggaagcccaa
     2581 ttgctcgcca atcggggtta cgccgtgctc cagatcaatt accgtggctc gaccggatac
     2641 ggaaagcgtt tctacaacgc ggcggtgaag gaattcgccg gcgccatgca caatgacctg
     2701 atcgacggtg tgaactgggc tgtgaaacag ggcttggtgg atccgaagaa gatgtgtgtg
     2761 tacggcggat cgtatggcgg atatgcgacc ctggtcagcg tgaccttcac gcccaagcag
     2821 ttcacctgcg ccgtggatta cgtgggtccg tcatcgctga tcacgctggt cgagtcgttt
     2881 cccgcgtact ggcggccgtt cctcgagggt agctggtatc gttttgtcgg cgatccggca
     2941 aaacccgaag accgaaagga catgtgggcc cgctccccgc tgtcccgggt agaccagatc
     3001 accacgccgc tcgtgattgt ccagggcgcg aacgatccgc gggttaccaa gaaggaagct
     3061 gatcagctgg ccgtggcgct gcggcaacgc ggagtgaagg tgcagtatct catcgctgcc
     3121 aacgaggggc atggattcct gaatccggac aaccggctcg cactgtacca cacgatggag
     3181 cggttctttg cgcagtactt gaagggccgc acccaggcca aaacgccggc cggggtggac
     3241 gcccggattc gagcgatgac ggtagacgtc gatacactca aggccggcca gggacaggat
     3301 cgcgccgcga cgcgatagcc ctagacccga gcttcggtcg aacctatcgt ttgaggctgc
     3361 tggtttaccc ttcgatcccg gcagaccgcg atggccgacc cccgcgccga gctggaacac
     3421 gcgctcagtg gccgttaccg gctggagcgc gagctcggcc gggggggcat ggccacggtc
     3481 tggctggctc atgacctcca ccacgatcgt ccggtagcac tcaaggtgct cgaccccggt
     3541 ctcgagcaat cgctcgggac tgagcgcttc cagcgcgaga ttcgactcgc ggcacgcctc
     3601 cagcatcccc acatcctgtc ggtctacgat tcgggcgaaa atgcggggca gctctggttt
     3661 acgatgccct tcgtggaggg agagtccctg cgcgaccggc ttcgccgcga gggaccgctc
     3721 ccggtcgggc aggcgctgcg catcactcgt gaggcggcgc aagcgcttca atacgcccac
     3781 gagcacggcg tggttcatcg ggatgtgaag ccggaaaaca ttctgctgac ccgcgatgga
     3841 agcacgctgg tcgccgactt cggcattgcg cgtcccctgg aaggcccgcc cgggacccag
     3901 ctcaccgtgg cagggttggt gatgggaacg ccgagctaca tgagcccgga gcaggcgacc
     3961 ggcagcacgg atctggacgg acgcagcgac atctactccc tcgcctgcgt gctgcacgag
     4021 atgctctcgg ggtctccgcc tttcaagggg tcgacgattc aatccgtcct cagcaagcac
     4081 atcaccgagt cgcctcctcc gctcacggca tcgaaccggg cgctccccgt ctcgatcgcc
     4141 cgtgccgtca cgaaggcgct ggccaaggtc ccggccgacc gattcaccag tgcagccgag
     4201 tttgcggcgg cgctggagcc gagcggagta gcgcgggcgt atcgaggacg acgcccgagg
     4261 agcagcgtcg tcgcgggcgt cggtggcgtg atcctggctc tggcaggctg gggactctac
     4321 gcaatgcgga gccgtgagac gtccgcacaa ccaagggaat cccggggaac gacgctcgca
     4381 acgggtttca atcgccagct gagccagctc accttcggtg aaggggtgga agagtggccg
     4441 gcatggtcgc ccgatggaaa gcgactggcg tacgtggccg aagccggcgg attcaagcaa
     4501 ctgttcctcc gcacgctggc aaacggaggg gaacaacagg tcactcacga cccgcgagac
     4561 cacatccttc ccaactggtc gcccgatggg aagcggcttg ccttcgtgcg cgcacgggcc
     4621 gactcgggaa cgctggatcc gagcgacctg gatgggtggt tctacgaagg cggtgacgtg
     4681 tggactatcg accttgcctc cgacagctcg agcaaactga tagacggcgc gttcggcccg
     4741 gcatacagtc cggacggaag ccggctcgca gtggacgcgg actgggccgg cccggaacgg
     4801 atctgggtgg tggatgccaa cggacgtaat ccccgtcaga tcaccaccga ttccagcgag
     4861 gcggtaatcc acgcaaagcc gcaatggtcg ccggacggga gcaagatcgc ctttcgccgg
     4921 atcgaaaaga ccaaatccga catcgtgatc gtggacgtcg cgacccaggc gatgacccgg
     4981 gtgaccgacg acaacgtggt cgatctcgat ccggtgtggt cgcgcgacgg gcagcggatc
     5041 tacttcagct catatcgggg cgggagcatc aacctctggt ggatcccggt cgcacccgac
     5101 ggtcacccca ccgcaggccc cagccagctc acgaccggcg ctggtgaaga cgtcgagccg
     5161 gcgatcgggc cggacggcca gcgcatcgcg ttcggcgttc gaggcatcaa ctcagacatc
     5221 tggcgcttgc cggtctctcc ggaaacgggc cgagccaccg gtgatccgtc cccggtcctg
     5281 gagacgacgc gggtcgagag tcgaggcgca tggtcgccgg atgggcgggt gatcgcgttc
     5341 aactcggatc gtctgggaga gatgaatctg tgggtccgga cgctggcgga cggctcggag
     5401 cgtcaactga cccggggtac gggaggcgac taccagccta actggtcgcc tgacggccgt
     5461 cgaatcgcat tcttctcggc ccgcggagga aacaccgaca tctgggccgt ggacgtggcc
     5521 gacgggaagc taacccggct gaccgacgat cgtgcgctgg acaccaatcc tttctactct
     5581 ccggacggca agcagatcgc cttcatgtcg gatcggaccg gacggtccga ggtctggatc
     5641 atgaacgcag acggctcgaa ccagcggcga ctcacgtcgg tggggacgtg gggccatttt
     5701 ctgcggtgga ccagggatgg acgaggtatc gtctttcgat cggagagcgc tgtcccggtc
     5761 caggtctatc ggatgtcagt tgcggatgga agcctcacga agttgcccga cgtgctgagc
     5821 ggcggacaca tgtccttctc accggaccag tccctcattc tcgacgtgca gggccacaag
     5881 acgatctggg tctatccggt aaacggtcag gcgccgtaca agatcttttc gtttccgtcg
     5941 cccgacgtgc ggatcgacta tccggtgtgg tcgccggacg ggcggtgggt gctgttcgat
     6001 cacgcggcgc cgaggggcgg ggatttgtgg atgctggaac tgggtgcgag ggctagcgag
     6061 tagctaccag ctatcagcta tcagctatca gaagttggcc attagccggc gatggccgtc
     6121 tgagacgcct gctcggcccc gcaactctga tagctgatag ctgatagctg atagctactt
     6181 cgctgatagc tacttctccc cctcgtcctc ctcttgcacc gactccatcg ccgccttcaa
     6241 atccgtcgcc agggtacgct cccgccggag gacctccacc aacgcctccg cctgcttcat
     6301 ctggaaggcc agggcaccgg cgcgttcgat cacgtgatcc accacagttc gctgcgcctg
     6361 gagcgactcg accgtagcgc ggacgtccac cgccaacgct tcggctcgcg ccagccgctg
     6421 ctcggcgcgc tccagctgcc gccggcgtga ttcgaagccg ttgagggctt cctccacacc
     6481 cttgaactgt gcctgggtgt cttggagcag ctccctcgtc tcttccacct gccggcgcgc
     6541 ggcgcccagc tcctgcacgt gctcgacggc gcgctcggcc atttcgaaga catgtttcac
     6601 ctgggcttcc aacgcctcga cggctccctg gcgtgcgagc gtctgctcca acccacgagc
     6661 agcctcggcc tgagccgact cccacttggc gagcagctcc tcgaagtggt tgagctggcg
     6721 atcgatactc ttgagtgcag tggcacgggc ctccagatcg
//
LOCUS       DASRJQ010000697         7905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_1785507, whole genome shotgun sequence.
ACCESSION   DASRJQ010000697 DASRJQ010000000
VERSION     DASRJQ010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7905
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1785507"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VFB89_15760"
     CDS             complement(<1..441)
                     /locus_tag="VFB89_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28805.1"
                     /translation="MTTESRLMDAVDRRDRWSLVAVGIVALGARLAVLPFATSDGGDA
                     PSKVWMASEWLSHPQLLTHGVWGPLHTYLIALALWTVPDPVRAPVVMSLMFSVASAVA
                     MYWFLRTEVASRRAALLVGLTYAVYPIAIRNGVSVRSETPFAFFL"
     gene            552..1355
                     /locus_tag="VFB89_15765"
     CDS             552..1355
                     /locus_tag="VFB89_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28806.1"
                     /translation="MAHARLPLVQRRFGETMRRDAWWVQPLVVFVLLSAFVVYATWAA
                     FQGDHYTYGPYLSPFYSPELFGSSPHAWFGPKPGWWPHWMVFSPALLILPFPGLFRLT
                     CYYYRGAYYKAFWADPPACAVGEPRKQYCGEAAFPLILQNVHRYFLYIALGFLVVLSH
                     DVYQALWFTDPATGHTSFGIGLGTLVLAANVTALGCYTLGCHSMRHVIGGYRDRLSRS
                     PVRKTAYDCSSCLNRAHMRWGWTSLFLVAFADLYVRMCSMGVLTDWRIL"
     gene            1392..3212
                     /locus_tag="VFB89_15770"
     CDS             1392..3212
                     /locus_tag="VFB89_15770"
                     /inference="COORDINATES: protein motif:HMM:NF005866.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HZM28807.1"
                     /translation="MADYQTFEHDVLVIGAGGAGLRAAIAAAAAGVSVGLVCKSLLGK
                     AHTVMAEGGIAAALANVDDRDSWRVHFADTMRGGQYVNNWRMAELHAKEAPDRVRELE
                     AWGALFDRTPDGRILQRNFGGHKYPRLAHVGDRTGLEMIRTLQDYTIHQSIDIHMECT
                     VLRLFTGGARIAGALGYDRERGRFRLYRCKAVVLATGGVGRAYSITSNSWEYTGDGHA
                     LAYQAGAALQDMEFVQFHPTGMIWPPSVRGILVTEGVRGEGGVLLNNQGRRFMFDDIP
                     ENYRSQTADNEEEGWRYTQGDKNARRPPELLTRDHVARCIMREVREGRGSPHGGVFLD
                     ISWIRKKLPNASGHIIKKLPSMYHQFKQLADIDITTTPMEVGPTTHYIMGGIRVDGDS
                     QMSTLPGLFAAGECAAGLHGANRLGGNSLSDLLVFGKRAGDYAAAFAREHDVAPLDSA
                     QADQAARAALAPFERGPSANGSGAYQVQQELQTMMQELVGIVRRRTEMEQALDNIERL
                     RARARQVGVGGNREYNPGWHTALDLENLLTISEAIARAAIERKESRGGHFREDYPEKD
                     PAASKFNIVIRKAADGRMQLIREPIPAMPAALQQVIEEQK"
     gene            3217..4050
                     /locus_tag="VFB89_15775"
     CDS             3217..4050
                     /locus_tag="VFB89_15775"
                     /inference="COORDINATES: protein motif:HMM:NF009052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HZM28808.1"
                     /translation="MTETLKPDLVETGAVEEREPPQARPEPHGTATFRIWRSEGASGR
                     LVDYTTRVSPGMVVLDAVHQIQAEQANDLAVRWNCKAGKCGSCSAEVNGTPKLMCMTR
                     LSDLPLDKPVTVEPMRSFPPIKDLVTDVSWNFRVKEKIKPFKPRKPDAPDGTWRMYQA
                     DVDRVQEFRKCIECFLCQDVCHVLRDHRLHEQFIGPRFLVHVAALEMHPLDVEDRVAD
                     LRQADGIGYCNITKCCTKVCPEHITITDNAIIPLKERVVDQFYDPLGRLLKVFSRKAE
                     P"
     gene            4047..4559
                     /locus_tag="VFB89_15780"
     CDS             4047..4559
                     /locus_tag="VFB89_15780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28809.1"
                     /translation="MTAMDEIKPISREAIPLALQKAERYRIIADPSSAESICLDVLGV
                     EPDNQQALVMLLLAITDQFADGPTEGVRRARELLPRLADRYRQAYYAGIICERCGKAL
                     VRRDTPGSGELAYPWFKEAMSWYEKAEGMRPAGNDEAILRWNTCARILANNDQLRPRE
                     EEAPVEPSLE"
     gene            4569..5516
                     /locus_tag="VFB89_15785"
     CDS             4569..5516
                     /locus_tag="VFB89_15785"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HZM28810.1"
                     /translation="MAEAKVIGVVGATGAQGGGLVRAIQRDKSGGFRARALTRKTTSD
                     QAKELVKLGAEVVAADLDDQASLEKAFKGASVVFCMTNYWEHFSPEKELAQATTQARA
                     AKQAGVPHVIWSTLEDTRRWVPLHDDRMPTLQGKYKVPHFDAKGEADRVFSELVPTTL
                     LLTSFYWENFIFFGAGPKPGPDGKLALTFPLGDKKLPGIAVEDIGKCTYGLIKRGREF
                     IGKTVAISGEQLTGAQMAAAMTKALGREVRYNDVPPEVYRGFGFPGADDLGNMFQFKR
                     DFQEAFCGPRDPAFARSLNPELQTFDQWLARNKDRIPLD"
     gene            complement(5533..6438)
                     /locus_tag="VFB89_15790"
     CDS             complement(5533..6438)
                     /locus_tag="VFB89_15790"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZM28811.1"
                     /translation="MKGIRSGFRALQSVSPAAAAWLAERLFFTPPRQRSELPAAIEAL
                     LARARRFELRVERRRVVGWRWGEGPAVYLVHGWGSRANRLLGLAPPLLDAGYSVVAFD
                     APGHGASGRGMSSIPEFARTLEAVVAEQGAAHAVIAHSLGAAATALAVAWGLAVRRLV
                     FLAPPADPASWVHAFAQRLGLRNDVVARLRARSEARLRMRWADLNIPRAAQGMAVPLL
                     VAHDENDEKVAWRDGAEIAAAWPGARLITTTGLGHGGVVRAPAVARDVVAFATAPEPG
                     PPWHDESERLECELFYRELRWAHAV"
     gene            complement(6435..7025)
                     /locus_tag="VFB89_15795"
     CDS             complement(6435..7025)
                     /locus_tag="VFB89_15795"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZM28812.1"
                     /translation="MSKGAATRQTILTHAAGLASQVGLTGLTIGGLADDLRLSKSGLF
                     AHFRSKEALQVEVLEHAAARFTDQVVRPALQKRRGVPRMRALFERWLAWEQAEVLPGG
                     CVFVAAASELDDRPGPVRDALVTLQRQWVDVLARSFRYGIEERQFRADADPEQFAQDL
                     YGIMLAYHHHARLLADPLAERRARRAFRRLLEDARR"
     gene            7173..7625
                     /locus_tag="VFB89_15800"
     CDS             7173..7625
                     /locus_tag="VFB89_15800"
                     /inference="COORDINATES: protein motif:HMM:NF021495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HZM28813.1"
                     /translation="MRWFLAVTHLLGLGVGLGAVWARGRALRSELDVAGLRRTFAADN
                     WWALAAILWIASGLVRAFGGSEKGSAYYLHNHLFLTKMVLLGILLILEIRPVVTLMRW
                     RTQVRRGERPDTQAAPTLARISFWQAGLVVLMVIAATGMARGYGVPHP"
     gene            7622..>7905
                     /locus_tag="VFB89_15805"
     CDS             7622..>7905
                     /locus_tag="VFB89_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28814.1"
                     /translation="MTAPDTPPPPAAELSQPRDPTVPLCEVLEQELTALTGQPIEPSY
                     DWRFTAEQILDPVRLAARLRDGKDRASRRLARGQFAPLVERLLTLGPDAEP"
BASE COUNT         1406 a         2637 c         2561 g         1301 t
ORIGIN      
        1 caggaaaaag gcgaacggcg tctcggatct gacggacacg ccgttccgaa tcgcaatcgg
       61 atacaccgcg taagtgagtc ccaccaggag cgcggcgcgt cggctcgcga cctcggtgcg
      121 aagaaaccaa tacatggcca ccgcgctcgc taccgagaac atcaggctca tcaccacagg
      181 ggcccgcacc gggtccggga cggtccagag ggcaagcgca atgaggtagg tatgtagcgg
      241 gccccagact ccatgcgtca gcagctgggg atgcgacagc cattccgagg ccatccacac
      301 tttcgacggt gcgtcaccac cgtcgctggt ggcgaacgga agcacagcca gccgcgcacc
      361 cagtgcgacg atcccaaccg ctacgagcga ccagcgatcc cgacggtcga cggcatccat
      421 gaggcggctc tccgtcgtca cggcattgac acactgctgg cagcgctcaa atatccgtct
      481 ggttcgcgca ttcggtggcc aggtccactt cgatcgggtg ccgcgggtct ccgcggggcc
      541 gggtattact catggcacat gccagacttc cgctggtgca acggcgtttc ggcgaaacca
      601 tgcgccgcga tgcctggtgg gtgcaaccgc tcgtggtctt tgtactgctc tcggctttcg
      661 tcgtgtatgc cacgtgggcc gcattccagg gcgaccacta tacctacggt ccgtacctct
      721 cgcctttcta ctcgcccgag ctgttcggca gctcgccaca cgcgtggttt ggaccgaagc
      781 cgggctggtg gccgcactgg atggtattct cccctgccct cctgatcctg ccgtttcccg
      841 gactctttcg gctgacgtgc tactactatc gtggcgcgta ctacaaggcg ttctgggccg
      901 atccgccggc ctgtgcagtc ggcgagccgc gcaagcaata ctgcggcgag gccgcgttcc
      961 cgctcattct gcagaacgtt caccgatatt tcctctacat cgcgctgggt ttcctcgtcg
     1021 tgctgagcca tgacgtgtac caggcactct ggttcaccga tccagcgacc ggccacacct
     1081 cgtttggaat cgggctgggg acactcgtgc tggccgccaa tgttaccgcg ctcggctgct
     1141 acacgctggg atgccattcg atgcgccacg tcatcggggg atatcgcgac cggctctccc
     1201 ggtcaccggt ccgtaagacc gcgtacgact gctccagctg tctgaaccgc gcccacatgc
     1261 ggtggggctg gaccagcctc tttttggtcg cctttgccga tctctatgtc cggatgtgct
     1321 cgatgggggt gttgacggat tggcggatat tgtgaagcaa gctatcagct atcagctatc
     1381 ggctatcagc tatggctgat tatcagacgt tcgagcatga tgtcctggtg atcggggccg
     1441 gcggcgccgg gctccgggct gccatcgccg cggccgcagc gggcgtttcg gtcggcctcg
     1501 tctgcaagtc gctcctggga aaagcgcaca ccgtcatggc cgaaggcggg attgcggcgg
     1561 cccttgccaa cgtggacgac cgcgacagct ggcgcgttca tttcgccgac accatgcgcg
     1621 gcggacagta cgtcaacaac tggcgtatgg ccgagctcca cgccaaagaa gcgccggacc
     1681 gggtgcgcga gctggaagcc tggggcgcgc ttttcgatcg aaccccggac ggtcgaatcc
     1741 tgcagcgcaa ctttggcggc cacaagtatc cgcggctggc gcacgtaggc gaccgcaccg
     1801 gtctcgagat gatccgcacg ctccaggatt acaccattca tcagagcatt gacattcaca
     1861 tggagtgtac ggtgcttcgc ctgttcaccg gtggtgcgcg catagccggc gcgctgggct
     1921 acgaccgcga gcgcgggcgc ttccgcctgt accgctgcaa ggccgtcgtc ctcgcaaccg
     1981 gcggcgtcgg tcgagcctac tccattacga gcaacagttg ggagtacact ggcgacggcc
     2041 atgctctcgc gtaccaggcc ggcgctgcgc ttcaagacat ggagttcgtg cagttccatc
     2101 ccaccggcat gatctggcca ccgagcgttc gcggcatcct cgtgaccgag ggcgtacggg
     2161 gcgagggcgg cgtcctgctc aacaaccagg gccgtcgctt catgttcgac gatattccgg
     2221 agaactatcg cagccagact gccgacaacg aggaagaggg ctggcgctac acccagggcg
     2281 acaagaacgc tcggcgaccg cccgagctgc tcacccgcga tcatgtcgcc cggtgcatca
     2341 tgcgcgaggt tcgcgaaggt cgaggcagcc cccacggagg cgtgtttctc gacatctcct
     2401 ggatcaggaa gaagcttccc aacgcctccg ggcacatcat caagaagctc ccgagcatgt
     2461 accatcagtt caagcagctc gccgacatcg acatcaccac cacgccgatg gaagtcggac
     2521 cgaccactca ctacatcatg ggtggaattc gggtcgacgg cgacagtcag atgtctacgc
     2581 tgccggggct ttttgcggca ggcgaatgtg cggcgggcct gcacggggcc aaccggctgg
     2641 gcggcaattc cctctcggat ctgctggtct tcggcaagcg cgccggcgat tacgccgccg
     2701 cgttcgcccg ggagcatgac gtcgctccgc tcgactccgc tcaggccgat caggccgctc
     2761 gcgccgccct agcaccgttc gagcgcggcc cttccgccaa tgggagcggg gcttaccagg
     2821 tgcagcagga gctccagacc atgatgcagg agctggtcgg catcgtgcgg cgccgaacgg
     2881 aaatggagca ggcgctcgac aacatcgagc gcctcagggc gcgtgcacgc caggtaggtg
     2941 tcggcggtaa tcgggagtac aatccgggct ggcacacggc gctcgacctc gagaatctgc
     3001 tgacgatctc cgaggccatc gcccgggccg ccatcgagcg gaaggaaagc cggggcggcc
     3061 actttcgcga ggactatccc gagaaggatc ctgccgccag caagttcaac atcgtgattc
     3121 ggaaggccgc ggatggccgc atgcagctca tccgcgagcc gatccctgca atgccggcgg
     3181 cgttgcagca ggtgatcgag gagcagaagt aggatcatga ccgagacgtt gaaaccggac
     3241 ctggtcgaaa ccggcgccgt cgaggagcgc gagcccccgc aggccaggcc ggagccgcac
     3301 ggcaccgcca cgttccgcat atggaggagc gaaggtgcca gtggccggtt ggtcgactac
     3361 accacccggg tctcgcccgg catggtcgtc ctcgatgcgg ttcatcagat tcaggcggag
     3421 caggccaacg atctcgcagt ccgctggaac tgcaaggccg gcaagtgtgg ctcctgctcc
     3481 gccgaagtga acggcacgcc caagctgatg tgcatgacgc gtctcagtga cctaccactc
     3541 gacaagccgg tcacggtcga gccgatgcgg agcttcccgc cgatcaagga tctggtcacc
     3601 gacgtgtcgt ggaatttccg ggtgaaggaa aagatcaagc cgttcaagcc gcggaagccg
     3661 gacgcaccgg acgggacctg gcggatgtac caggccgacg tggaccgggt gcaggagttc
     3721 cgcaagtgca tcgaatgctt cctctgccag gacgtctgtc acgtgctgcg ggatcatcgc
     3781 ctgcacgaac aattcatcgg cccccggttc ctggtgcacg tggcagcgct cgagatgcat
     3841 ccgctcgacg tcgaggatcg ggtcgcggac cttcgccagg ccgacggcat cggttactgc
     3901 aacatcacca agtgctgcac caaggtctgc ccggagcaca ttacgattac cgacaacgcg
     3961 atcattccgc tcaaggaacg tgtcgtcgat cagttctatg atccgctggg gcgcctgctc
     4021 aaggtgttca gtcgcaaagc agagccgtga ccgccatgga cgagatcaag cctatttctc
     4081 gggaggccat tccgctggcg ctgcagaagg cggagcgata ccggatcatc gccgacccat
     4141 cgtccgccga aagcatctgc ctcgatgtgc tgggcgtaga gcccgataac cagcaggccc
     4201 tcgttatgct actcctcgcg attaccgatc agtttgccga cggccccacc gaaggcgtcc
     4261 gccgcgcgcg ggagttgctg cctcgtctcg ccgatcggta tcgccaggcg tattacgcgg
     4321 gcatcatttg tgaacgctgc ggcaaggctc tcgtgcggcg ggacacgccc ggttccgggg
     4381 agctggcata tccctggttc aaggaagcga tgagctggta cgaaaaggcc gaggggatgc
     4441 ggccggccgg taatgacgaa gccattctgc gctggaacac ctgcgcacgc attctcgcaa
     4501 acaacgatca gcttcgaccg cgggaggaag aagctccggt cgagccaagc ctagagtagg
     4561 aggaacgcat ggcagaagcg aaggtgattg gcgtggtcgg cgccaccggg gcgcagggcg
     4621 gtggactggt tcgcgcgatt caacgagaca agtccggtgg gttccgggcc cgggcactta
     4681 cccggaagac cacctcagac caggcaaagg aacttgtgaa gctgggcgcg gaagtcgttg
     4741 ctgcagacct agatgaccag gcaagcctcg agaaggcgtt caaaggtgcc tcggtcgtgt
     4801 tttgtatgac gaactactgg gagcacttct cacccgagaa ggagctggcc caggccacca
     4861 cccaggctcg cgcagcgaag caggccggcg ttcctcatgt gatctggtcg acgctggaag
     4921 acactcggcg ctgggtgccg ctccacgacg atcgcatgcc gaccttgcag gggaagtaca
     4981 aggtccccca tttcgacgcc aagggggagg cagacagggt cttcagcgag ctggtgccga
     5041 ccacgctgct gctcacgtcg ttctactggg aaaacttcat cttcttcggc gccggaccga
     5101 agccgggccc ggacggcaag ctggcgctca cctttccgct cggcgacaag aagctgcccg
     5161 gtatcgctgt ggaggatatc ggcaaatgca cctacggcct gatcaaacgc gggcgtgagt
     5221 tcatcgggaa gaccgtggcc atttcgggag agcagctgac cggcgcccag atggcggccg
     5281 caatgacgaa ggcgctgggt cgggaggtgc gctacaacga cgtgccgccg gaggtctacc
     5341 gcggcttcgg tttccccggc gccgacgacc tgggcaacat gttccagttc aaacgcgact
     5401 ttcaggaagc gttctgcggc ccccgggatc cggcatttgc ccgcagcctg aatcccgagc
     5461 tgcagacgtt cgaccagtgg ctcgcgcgga acaaggatcg catcccgctc gattgatgcg
     5521 ctgcctccgc ggtcagaccg cgtgcgccca tcgaagctcc cggtagaaca gctcgcactc
     5581 caggcgttcc gactcgtcat gccacggcgg cccgggttcg ggcgccgtgg caaaggccac
     5641 cacgtcccgc gccaccgcgg gagcacgcac gacgcctcca tgtcccaagc cggtcgtcgt
     5701 aatcagtctg gcacccggcc aggcggcggc gatctcggca ccgtcgcgcc aggcgacctt
     5761 ctcatcgttc tcgtcgtgcg cgaccaggag cgggacggcc atgccctgcg ccgctcgcgg
     5821 gatattgagg tcggcccagc gcatgcgcag tcttgcctca ctccgggccc gtagtcgcgc
     5881 gaccacatcg ttccgcaagc cgagccgttg ggcaaaggcg tggacccagc tagccggatc
     5941 ggccggcggt gccaggaaga ccagccgacg cacggcgagg ccccatgcga cggccagtgc
     6001 cgtggccgcg gcaccgagcg aatgggcgat cacggcgtgg gcagcgccct gctcggcgac
     6061 caccgcctcg agcgtccggg caaactccgg aatcgagctc atgccccgcc ccgacgcgcc
     6121 gtggccgggc gcgtcgaagg caaccaccga atagccggcg tcaagcagcg gcggagccaa
     6181 cccgagcagc cggttcgcac gcgatcccca tccgtgcacc aggtagaccg caggtccctc
     6241 gccccaacgc cagccaacta cccgccgccg ttcgactcgg agctcgaaac gccgcgctcg
     6301 cgcaagcagc gcctcgatcg cggcgggcag ctcgctccgc tgacgcggcg gggtaaagaa
     6361 cagccgctcc gcaagccacg ccgcggccgc aggcgagacc gattggagcg cacggaagcc
     6421 ggagcggatc cctttcatcg acgcgcatcc tccagcagac ggcgaaacgc ccgacgggcc
     6481 cggcgctccg cgagcggatc agccaggagc cgtgcgtggt gatggtatgc gagcatgatt
     6541 ccgtacaggt cctgggcgaa ctgttcggga tcggcgtcgg cgcgaaactg tcgctcctcg
     6601 atcccgtaac ggaacgagcg cgccaggacg tccacccact ggcgctgcag cgtcaccagc
     6661 gcatcgcgca ccggtcccgg ccgatcatcc agctcgctcg ccgccgccac gaacacgcag
     6721 ccacccggca gcacttccgc ctgctcccag gccagccagc gctcgaacag cgcccgcatc
     6781 cggggaacgc cgcgccgctt ctgcagtgca ggccggacga cctggtcggt gaaccgggcc
     6841 gcggcatgct cgaggacttc cacctgtaga gcttccttgg agcggaaatg ggcaaagagc
     6901 ccgctcttgg agagccggag gtcgtcggcc aggccgccga tggtgaggcc ggtcagaccg
     6961 acctggctgg ccaggccggc ggcgtgggtg aggatggtct ggcgggtggc ggcgcccttg
     7021 gacatgccgg cgaaaatagc acgaccgttc gtgctgtcaa tgcccgggca ccgcgaccgg
     7081 atcggccact caacgacggt agacgcggcc cccgcgattc gtagctttcc cccgctggtc
     7141 gcctaacgcc tcaacccagc gggtacagga ccatgcgctg gttcctcgcc gtcactcatc
     7201 tgctcggcct cggcgtcggc ctgggcgccg tatgggctcg gggccgagcg cttcgcagcg
     7261 agctggacgt agcgggcttg cgccgcacgt tcgcggcgga caactggtgg gccctggccg
     7321 ccatcctgtg gattgcaagc ggcctggtcc gagccttcgg cgggtcggaa aagggcagcg
     7381 cgtactacct ccacaaccat ctctttctca ccaagatggt gcttctcggc atccttctga
     7441 tcctcgagat ccgtccggtc gtgacgctga tgcgctggcg tacccaggtg cgccgcggcg
     7501 agcgaccgga cacgcaggcc gcgccgacgc tcgcacgcat cagcttctgg caggcgggac
     7561 ttgtcgtcct gatggtcatc gccgccacgg gtatggcccg cggctacggc gtaccgcacc
     7621 cgtgacggcg ccggacaccc ctccgccccc ggcggcggag ctttctcaac cgcgcgaccc
     7681 caccgttccg ctgtgcgagg tgctcgagca ggagctcacc gctctcaccg ggcaaccgat
     7741 cgagccttcc tacgactggc gcttcacggc ggagcagatt ctcgacccgg tgcgcctcgc
     7801 cgctcggctg cgggatggga aggaccgtgc ctcgcggcgg ctggccagag ggcagttcgc
     7861 tccgctggtg gagcgtctgc tgactcttgg cccggatgcg gagcc
//
LOCUS       DASRJQ010000698         3151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_136266, whole genome shotgun sequence.
ACCESSION   DASRJQ010000698 DASRJQ010000000
VERSION     DASRJQ010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136266"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /gene="hisH"
                     /locus_tag="VFB89_15810"
     CDS             <1..496
                     /gene="hisH"
                     /locus_tag="VFB89_15810"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HZM28815.1"
                     /translation="VLVPGVGHFGQAAANLDRSGLGAAVCEAAASGRPVMGICLGLQL
                     FLESSEEAPGVPGLGLLSGNATRFKVDLPIPHVGWARVEATAAGRAHGMLRAVLETDF
                     FYHVHSYHPSGLSNGAVLATGEYGYVFPTIVGEGNVIGAQFHPEKSQRAGLDLLGAFA
                     RWRP"
     gene            493..960
                     /locus_tag="VFB89_15815"
     CDS             493..960
                     /locus_tag="VFB89_15815"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZM28816.1"
                     /translation="MTTVRTKFVDVYVLRGSAAALEVLLLRRSSGGRCPGSWETVHGH
                     IESAESPADAARRELTEETGLEAERLYNLSRVELFYQHRPDEVALVPVFAAFVDAGAA
                     VRLSSEHDRQEWLRPEEARTRFAWPRERRALDDILILLREGDAGPLSDALLVC"
     gene            954..1712
                     /gene="hisF"
                     /locus_tag="VFB89_15820"
     CDS             954..1712
                     /gene="hisF"
                     /locus_tag="VFB89_15820"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HZM28817.1"
                     /translation="MLARRIIPCLDVAGGRVVKGVHFEALRDAGDPVAQAARYDAEGA
                     DELVFLDISASHEERRTTLEMVSRVADTVFIPVTVGGGIRSVRDGGDALRAGADKIAV
                     NTAAVRDPPLVERLAHSFGTQCVVAAVDAKRINGRFTVMVTGGREQTSLEAIEWIRKL
                     ESLGAGEILLTSMDRDGTQSGYDLPLLRAAAAAVRIPLIASGGAGKLEHLAEALEAGA
                     HGVLAASIFHFRGCSIPEARTFLREKGFPVRGER"
     gene            complement(1734..2351)
                     /locus_tag="VFB89_15825"
     CDS             complement(1734..2351)
                     /locus_tag="VFB89_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28818.1"
                     /translation="MQFLLPLCVLVLLSACTGEPTPAAAERRAGEPLAEATPVHVDSF
                     IPRDEALRRFRRGLPPTNGLSHGMASRESLVRAFVRALERRDSTTLRQLVLSRAEFAY
                     LYYPSAPQGLPPYDMNADLMWFMLSIQSEKGMHRALNERGGSPLYVVGTRCNEKPDTQ
                     GENTLWGPCVLRRIEAPGDTLDERLFGPIIERQGSYKFLTYANKL"
     gene            complement(2429..>3151)
                     /locus_tag="VFB89_15830"
     CDS             complement(2429..>3151)
                     /locus_tag="VFB89_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28819.1"
                     /translation="WTEGYKGRNQFLIGLQTQRLVPAPGAGVFSSDPRGFEGDGCDPA
                     VSGCTLTSTGASTPYSMPVFGNFTLVGPGQAVGVADGNGAVLRRGTGGTFINGILARW
                     KGIAINVRDDWTDSFFVASGGKDSLQITNTLLAQNGFNFDTVGKDHAQTTNWNPTRLA
                     TIKQYAADVLVDTLLGFNLNPTGLNWSPKAGAPSNTGGGTAPAARVANYFGGSWQNTS
                     YLGAAQSNGAQWWLGWTAYAIN"
BASE COUNT          524 a          993 c         1056 g          578 t
ORIGIN      
        1 agtgctcgtg ccgggcgtgg gtcatttcgg ccaggcggcg gccaacctgg accgcagcgg
       61 acttggcgcg gccgtttgcg aggcagccgc gtcgggtcgt ccggtcatgg ggatctgtct
      121 aggcctccag cttttcctcg agtccagcga agaggcgccc ggcgtccccg ggctcggact
      181 cctctccggc aacgcaaccc gcttcaaggt cgacctgccg attccccatg tcggctgggc
      241 cagggtcgag gcgactgcgg ccgggcgcgc acacgggatg cttcgcgccg tgttggagac
      301 cgacttcttc taccacgtcc acagctacca tccttccggc ctgtccaatg gagcggtgct
      361 cgcgaccggc gagtacggct atgtgttccc gacgatcgtg ggcgagggca atgtgatcgg
      421 cgcccaattc catccggaga aatcgcagcg ggccgggctc gatctgctgg gtgcgtttgc
      481 ccgctggcgt ccgtgaccac cgtacgtacc aagtttgtgg acgtgtacgt tctgcggggt
      541 tcggcggcag ccctggaggt gctgcttctg cgccgcagct ccggcgggcg atgtcccggc
      601 tcctgggaga cggtgcacgg acacatcgag tccgccgaat cgccggctga cgcggcgcgc
      661 cgggaactga cggaagaaac gggtctggaa gcggaacggc tctacaacct gagccgggtg
      721 gagcttttct atcagcaccg gccggatgaa gtcgctctgg ttcccgtatt cgctgcgttc
      781 gtggacgccg gcgccgcggt tcggctgagc tccgagcacg atcgccagga gtggcttcgt
      841 cccgaggagg cccgcactcg cttcgcatgg ccgcgcgaac gccgtgcgct cgacgacatc
      901 ctcattctgc ttcgcgaggg tgatgccgga ccgctcagcg atgccttgct ggtatgctag
      961 cccgacgaat cattccctgc ctcgacgttg ctggcgggcg ggtcgtgaaa ggagtgcact
     1021 tcgaggcgct gcgcgacgcc ggagatcctg tcgcgcaggc agcacggtac gatgcagaag
     1081 gcgccgacga gctggtcttt ctcgacatct ccgccagtca cgaagagcgg cgtacgacgc
     1141 ttgaaatggt gagccgcgtg gcggataccg tgttcatccc ggtaacggtg ggagggggca
     1201 tccgctcggt ccgtgatggc ggagatgccc tccgcgccgg tgccgacaag atcgcagtga
     1261 acacggccgc cgtacgagat ccgccgctgg tcgaacgact cgcccacagc ttcgggacgc
     1321 aatgcgtggt ggcggcggtc gacgccaaac gaatcaacgg acgctttacc gtgatggtca
     1381 ccgggggccg ggaacagact tcgctcgaag cgatcgagtg gatccgcaaa ctcgaatccc
     1441 tcggcgccgg cgagattctc ctcacctcga tggatcgcga cggcacccag tccggatacg
     1501 acctgcccct gctccgtgcc gcggctgcag ccgtccgcat cccgttgatc gcgtcgggtg
     1561 gggcggggaa gctcgagcat ctggcagagg cgctcgaggc cggtgcccat ggagttctgg
     1621 cagcttcgat ttttcacttt cggggctgct cgattcctga agcgaggacc ttcctgcgcg
     1681 agaagggctt ccccgtgcgg ggagaacgat aagcgttctg tgctacactg aagtcagagc
     1741 ttgttcgcgt aggtgaggaa cttgtagctc ccctgccgct ctataatcgg cccaaagagt
     1801 cgctcgtcga gagtgtcacc gggcgcctcg attcgacgga ggacgcacgg gccccacagc
     1861 gtgttctcgc cctgggtgtc cggtttctcg ttgcagcgtg ttcccaccac atacaggggg
     1921 ctcccgccgc gttcgttcaa cgcccgatgc atcccctttt cgctctgaat gctgagcatg
     1981 aaccacatca ggtcggcatt catgtcgtag ggcggcagtc cctgtggcgc gctcggatag
     2041 tacagatagg caaactcggc tcgcgaaagg accagctgtc ggagggttgt cgagtcccga
     2101 cgctcgagcg cccgcacaaa tgctcgcaca agtgattccc ggcttgccat gccgtgcgag
     2161 agcccgttgg ttggcggcag cccacggcga aatcggcgca atgcttcgtc tcgcgggatg
     2221 aagctgtcca cgtggaccgg cgtcgcttct gcgagcggtt ccccggctcg acgctcggcc
     2281 gcggccggcg tgggctcgcc agtgcaggcg ctcagcaaca cgaggacgca gagtggaagc
     2341 aagaattgca tggagatggg gttcggccgc gccccagaga gcgcggccga taaaggttca
     2401 ggtcagatcg agcgaaatcg gaatcagatc aattgatggc gtacgcggtc cagcccagcc
     2461 accactgcgc tccgttcgac tgagccgcgc cgagatagct ggtgttctgc cagctgccgc
     2521 cgaagtaatt ggcgacccgt gccgcgggag cggtgccgcc gcccgtgttc gagggagccc
     2581 cggccttcgg tgaccagttg agcccggtcg ggttgagatt gaagccaagg agggtgtcca
     2641 ccagtacgtc tgcagcgtac tgcttgatgg tggccagacg agtcgggttc cagttggtgg
     2701 tctgggcgtg atccttgccg acggtgtcga agttgaaacc gttctgcgcc agcagggtgt
     2761 tggttatttg cagggaatcc ttgccgccag aggccacgaa gaacgagtca gtccagtcat
     2821 cccggacatt gatggcgata cccttccagc gagcaaggat gccgttgata aacgtcccgc
     2881 cggtaccgcg gcgcaacacg gcgccatttc cgtcggctac gcctaccgcc tggcctgggc
     2941 cgaccaaggt gaagttgccg aataccggca tcgaataggg ggtgctcgcg ccggtcgagg
     3001 tcagggtaca gccgctcacc gctggatcgc agccatcgcc ctcgaagccc cgcggatcgc
     3061 tcgagaacac gcccgcgccc ggcgccggca ccagccgctg ggtctgaagg ccgatcaaga
     3121 actggtttcg gcccttgtac ccttcggtcc a
//
LOCUS       DASRJQ010000699         2563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_223566, whole genome shotgun sequence.
ACCESSION   DASRJQ010000699 DASRJQ010000000
VERSION     DASRJQ010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223566"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VFB89_15835"
     CDS             complement(<1..297)
                     /locus_tag="VFB89_15835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28820.1"
                     /translation="MTTERWLLVAYACSGLAGLVYEITWTRLMTLHLGHTTAAVSTVT
                     AAFMGGLGLGGALGGRIASRLSRRQALRTYALLELAVALAAFSIAASMTALTPLF"
     gene            509..1564
                     /locus_tag="VFB89_15840"
     CDS             509..1564
                     /locus_tag="VFB89_15840"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZM28821.1"
                     /translation="MAKAVGALIAIAVVIFGASIMAQQQAAPAPAPAPTGPIPAGLPE
                     WAYTPPPAGGPPPPVSALPADDNAVVSIPGTTKTFTRGQLRAAKETMDWYPEDRHGTL
                     PDVARYGKQGVRQCTLCHLPDGSGRPENAPISSYHPTYFLQQMQDFRDGLRKSADPRK
                     ANTNNMINFAKATTREEDLAIAQYFAQQPYPRRIKVVESKTAPKVRLQGGMHMAVPAN
                     EGGGMEAIHADEIVEIPDDNLRAEARDTRMSWTAYVPPGTLNRGKQVATKYQCATCHG
                     ANLQGIGPVPALAGRSPSYTMRQLFDMKTGTRRGPWAELMKTVVSSMSVQDMAAVSAF
                     AASTLPPVAPTTSTASR"
     gene            1640..2050
                     /locus_tag="VFB89_15845"
     CDS             1640..2050
                     /locus_tag="VFB89_15845"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HZM28822.1"
                     /translation="MSRKLCAVVFAASVGLLVAVVPLLAHHSEAAEFDATKPVRVTGV
                     VKKVEWTNPHIWFYVEGKDEVSGTAAVWGFSGAAPNGLRRRGITRESLKVGDTVKVQG
                     IRAKDGSPNAASRGVTFSDGRQVFTASEQTPAPK"
     gene            2076..>2563
                     /locus_tag="VFB89_15850"
     CDS             2076..>2563
                     /locus_tag="VFB89_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28823.1"
                     /translation="MKRILRVGVVVALASIVVSAQTSKPAPRLPNGKPDFTGVWDHPR
                     VGDISKSVDGACAAGGAGCKSVVSGELLFTPAGKIEWDKNNKPTTYDYGSHCQPWGFV
                     RAYNTPYPHAYVHHPDRLVILWEQDNRFHVVPTDGRELPKDLEPTWMGTSTGRWDGDT
                     LVV"
BASE COUNT          508 a          799 c          834 g          422 t
ORIGIN      
        1 aaagaggggc gtgagcgcgg tcatgctggc tgcaattgag aacgctgcga gcgcgaccgc
       61 gagttcgaga agcgcataag tccgcagcgc ttgtcgacgg ctcagcctcg acgcgattcg
      121 accgcccaac gctccaccca gccccagccc acccataaac gctgcggtga cagtgctgac
      181 cgccgcggtc gtgtgtccga gatgaagagt catgaggcgc gtccaggtga tctcgtacac
      241 gagcccggcg agccccgagc acgcatacgc caccaggagc catcgctcag tggtcatcgc
      301 ggggagtgta gccgagccgt caggggacgg cctccagccc aaaagaattc tggacgctcg
      361 gggtccggac gtgctatcaa accgcctcgc gagtccgcgt ccattccagg tcgaaaaggg
      421 ggcggaacga ttgccgtatc tcaccctgca acatcctgta aactccgcgg aagcgagttt
      481 ctgctaacca caaatcatgg agaggaacat ggccaaagcg gtgggtgctc taattgctat
      541 cgcagtcgtg atcttcgggg cgtcgatcat ggcgcagcag caggccgctc ctgctcctgc
      601 tcctgctcca acaggcccca tccccgcagg cctcccggag tgggcgtaca caccgccgcc
      661 agcgggaggg ccgccacctc cggtctccgc actcccggcc gacgacaatg cggtggtctc
      721 gattcctggg acgactaaga cgttcacgcg cgggcagctt cgggccgcga aggagacgat
      781 ggattggtat cccgaggatc gtcacgggac ccttcccgac gtcgctcggt acgggaaaca
      841 gggcgtacgc cagtgcaccc tgtgtcacct cccggacggc tccgggcggc cagagaatgc
      901 tccgatcagc tcttatcacc cgacctattt cctgcagcag atgcaggatt tcagggacgg
      961 actgcggaag agcgctgatc cgcggaaggc gaacactaac aacatgatca acttcgccaa
     1021 ggcgacaacc cgcgaggagg atctggccat cgcgcagtat ttcgcgcagc agccgtaccc
     1081 gcgccgcatc aaagtcgtcg agtcgaagac ggcgccgaaa gtacggctcc agggcgggat
     1141 gcacatggct gttccggcca acgagggcgg cgggatggag gccatccacg ccgacgagat
     1201 tgtcgagatc ccggacgaca acctgcgagc tgaagcccgc gacacacgca tgtcctggac
     1261 ggcgtacgtg ccgcccggca cgctgaaccg cggcaagcag gtggccacga agtatcagtg
     1321 cgcgacatgc cacggcgcga acctccaagg catcggtccg gtaccagcgt tggcggggcg
     1381 ctcgccgagc tacacgatgc ggcagctctt cgatatgaag actgggacgc ggcgcggccc
     1441 atgggccgag ctgatgaaga cggtggtcag cagcatgtcc gtgcaggaca tggcggcggt
     1501 ctccgcattc gctgcctcga cgctcccgcc cgtggctccg acgacgtcga ccgcttcgcg
     1561 ctgaaaaccg ggccttcgtc tctggtctct ggactttggg ctggggacca gggacgatct
     1621 tgagcacggg agggctgtga tgtcacggaa actttgtgcg gtagtgtttg cggcgagcgt
     1681 cggactgctc gtcgcggtcg tccctctcct tgcgcaccac tctgaggcgg ctgagttcga
     1741 cgccaccaaa ccggtcaggg tgacaggtgt ggtcaagaag gtcgagtgga ccaatcctca
     1801 catctggttc tacgtcgaag gaaaagacga agtctcgggc acggcggcag tctggggatt
     1861 ctcaggcgct gcgcccaacg ggctcaggcg aagaggcatc acgagagaat cgctgaaggt
     1921 cggcgacacc gtgaaagtgc agggcattcg ggccaaggac ggttcgccga acgcagccag
     1981 ccgcggggtg acgttttccg acggccggca ggtgttcaca gcgtctgagc agactcccgc
     2041 tcccaagtag ctccagcgcg gaaagagaga tcacgatgaa gagaattctt cgcgtgggcg
     2101 tcgtggtggc gttggcatcg atcgttgtca gcgcccagac ttcgaagccc gcgccgcggt
     2161 tgccgaacgg caaacccgac tttaccggcg tgtgggacca tccccgcgtt ggcgacatct
     2221 caaagagcgt ggacggcgcc tgcgcagcgg gcggcgcggg ctgcaagagc gtcgtgtccg
     2281 gagaactgtt gttcacgccg gctggcaaga tcgaatggga taagaacaac aagccgacga
     2341 cctacgacta cggatcgcat tgccagccgt gggggttcgt acgggcgtac aacacgcctt
     2401 atccgcatgc gtacgtgcat caccccgatc ggctggtgat cctctgggag caggacaatc
     2461 gctttcacgt cgttcccact gatggacgcg agcttcccaa ggatctcgaa ccgacctgga
     2521 tgggtacgtc gaccggccgc tgggatggcg acacgctcgt cgt
//
LOCUS       DASRJQ010000700         2311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2190534, whole genome shotgun sequence.
ACCESSION   DASRJQ010000700 DASRJQ010000000
VERSION     DASRJQ010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2190534"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VFB89_15855"
     CDS             <1..447
                     /locus_tag="VFB89_15855"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM28824.1"
                     /translation="ITLRRERSKSGEPRTIPFVSTLADIIEARWTARECQAPAGPGLS
                     PFVFHRAGDPIKDFRGAWRKACTAAKQPGLLFHDLRRSAVRNFVAAGVDQAVAMRITG
                     HKTASVFQRYRIVADDDVRSALERTEAANNRTPASNVVELRPEAAG"
     gene            complement(529..617)
                     /locus_tag="VFB89_15860"
     tRNA            complement(529..617)
                     /locus_tag="VFB89_15860"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(581..583),aa:Ser,seq:tga)
     gene            complement(808..1089)
                     /locus_tag="VFB89_15865"
     CDS             complement(808..1089)
                     /locus_tag="VFB89_15865"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZM28825.1"
                     /translation="MNAVVRSLLVDIGARRQRPSDPTESIFAATYRTTARALEKAVIA
                     AQATLASTGQDASRLDGFTWHGLRHTWASRLVMAGVDLRTLQHWEAGGL"
     gene            1555..1719
                     /locus_tag="VFB89_15870"
     CDS             1555..1719
                     /locus_tag="VFB89_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28826.1"
                     /translation="MRLAWIVGGQHKVRFKLDKDRFPDFQLERDGQRCLSELVKGLFK
                     ELESRNRDLS"
     gene            1783..>2311
                     /locus_tag="VFB89_15875"
     CDS             1783..>2311
                     /locus_tag="VFB89_15875"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZM28827.1"
                     /translation="MTPVFDAICRSAVRLLRADQGGVFRFDGKLVHIAALTSLNPEAE
                     GRLRKRFLQVPNPHAGSGRAIVEKRVFQIPDTEEFPFEGQRATARDFGFRRSVWVPMI
                     REEQVIGVLSVNGRAPGLSDDQVHPDRHGGGTDLERRGWIKSLARPGGNVTGLIAFLP
                     EMGSKRIELVKDLLPR"
BASE COUNT          430 a          716 c          735 g          430 t
ORIGIN      
        1 atcacattgc gtcgtgagcg ttcaaaaagc ggcgagccgc ggaccatccc gttcgtgtca
       61 acgctcgccg acatcattga ggcccgttgg acagctcgcg agtgtcaggc ccccgccggg
      121 ccagggctat cgcctttcgt gttccatcgg gccggcgacc cgatcaaaga cttccggggt
      181 gcctggagga aggcatgcac cgcggcaaag caaccgggcc tgttgttcca cgatctcaga
      241 cggagcgccg ttcgtaactt cgtggccgcc ggcgtggacc aggcagtcgc gatgcggatc
      301 accggccaca aaacggcgag cgtcttccag cgctatcgga tcgtagctga cgatgacgtg
      361 cgctccgcgc tcgagcgcac cgaagcggcg aacaacagga cacctgcgag caacgttgtc
      421 gaactccgtc cagaagccgc cggctaggcg aaaagctcac cccgcgcggc ttcgggggac
      481 aatcgggggg caaaagtgcc ggttgaccac ggtgcccgtt ctgtaagttg gcggagggcg
      541 agggattcga acccccaagg gcctcgcggc ccggtggatt tcaagtccac tgccttgcca
      601 gttgaggcta gccctccgtg ctcgtagagt cgcacacttc cgcaccgttc tggattgaag
      661 cgtttcgccg ccggagcgag tcgttcaaat tccgttcaag tttagcggca ccgctgcgcg
      721 tcccgggatc ggctgacgtt acgaccaatg tctccatcgc cgccctcaag tgatcgggac
      781 tcagatggct gtggcgctcg accatcatca gagtcctcca gcctcccagt gctgcagcgt
      841 ccggaggtcc acgccagcca taacgagtcg ggaagcccaa gtatggcgga gtccatgcca
      901 ggtaaagccg tccagccggc tggcgtcctg tcccgttgac gccagggtgg cctgtgccgc
      961 gatcacggcc ttctcgaggg ctctcgccgt tgtccgatag gttgcggcaa atatcgactc
     1021 cgtgggatcg ctggggcgtt gccggcgagc tccaatgtcg acgagcagcg agcgcacgac
     1081 cgcgttcatt gggatgtgag cgttcgacca ttcttcgact ggaagatggt caacgtcgcc
     1141 gtcagaagat ctgcgtcgcg ccagcacaat cgggcctgct cagaccagcg catcccgtat
     1201 gaatcgccac agtgaccagc ggcctcaggt cgtgtggtag ggcgtcccgg aggacttgtt
     1261 catcgccggg cgtaagttac attacccgcc cagccggttc cttaagcttc gggacgcctg
     1321 ttaccggatt cgtcgtcacg agcccctggc gctgggcacg ctcgaagaac cctgagagca
     1381 gatcacggta tcggtttctg gttgccgggg acaccgaatg ggtgcccgcg aggagcgagc
     1441 ccaaaaaccg ctctatatcg ggtgtcttga tctcatcgag cctcttatca cccaaggcag
     1501 ggagcacccg ggccaagcgg gatgggtcct tcgcccatga gcggtgatgt gcccatgcgc
     1561 ctcgcgtgga tcgtcggcgg gcaacacaag gtcaggttca agctggacaa ggacagattt
     1621 ccggacttcc agctcgagcg cgacgggcaa cgctgccttt cggagctcgt caagggcctc
     1681 ttcaaggagc tggaatcccg caaccgcgac ctcagctaag cgctcgaaca acagacagcg
     1741 agcggtgaaa tcctccgcgt gatctcgagt tcaccgaccg acgtgacgcc tgtttttgac
     1801 gcgatctgcc gcagcgccgt tcggctcctc agggcggacc aaggcggcgt cttccggttc
     1861 gacgggaaac tcgttcatat cgccgctttg acgagcctga atccagaggc ggaaggtcgg
     1921 ctacgaaaga ggttcctcca ggtgcctaac cctcacgcgg gtagcggtcg cgcgattgtg
     1981 gagaagcggg tatttcagat tcccgacacc gaggagtttc ctttcgaagg acagcgcgcg
     2041 accgcacggg acttcggttt tcgtcgttct gtgtgggtcc ccatgatccg ggaggaacag
     2101 gtgattggcg tattgagcgt caatggccgc gcgcccggcc tttcggacga ccaagtccat
     2161 cccgatcgtc atggcggtgg aaccgatctc gagaggcgtg gctggatcaa gagcctggcc
     2221 cggccgggcg gcaacgtgac cggattgatc gccttcctgc cggagatggg cagcaagcgc
     2281 atcgagctcg tgaaggatct tctcccgagg g
//
LOCUS       DASRJQ010000701         4719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_2396997, whole genome shotgun sequence.
ACCESSION   DASRJQ010000701 DASRJQ010000000
VERSION     DASRJQ010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2396997"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..458
                     /locus_tag="VFB89_15880"
     CDS             120..458
                     /locus_tag="VFB89_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HZM28828.1"
                     /translation="MRRLCLALPEAHEVEAWGEPTFRVKNKLFAMYASAATHHGNGRP
                     SVWCKAADGNQGLMVRAAPDLFFVPPYVGPSGWIGVWLDRSPDWAELADLLRDAYLLV
                     APKRLVALLG"
     gene            501..1043
                     /locus_tag="VFB89_15885"
     CDS             501..1043
                     /locus_tag="VFB89_15885"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HZM28829.1"
                     /translation="MGHSTRPFDLFLALLSAHGIETVADVRRYPGSRRWPQFSRDALS
                     EALGAHGIAYQWFPDLGGRRDPAADSVNTGWRNKAFRGYADYMETGEFADGLVSLLNA
                     ALGSRTAIMCAEAVWWRCHRGLIADVLRWLGLDVRHIMSIESAELHPYTPPARIVDGR
                     LSYAADAPGRPSANFPRTTP"
     gene            1122..1442
                     /locus_tag="VFB89_15890"
     CDS             1122..1442
                     /locus_tag="VFB89_15890"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HZM28830.1"
                     /translation="MPVYLWQASYQAEGTKGLMKEGGSKRRQYVQQLVEKSGGKLLAF
                     YFAFGEIDAVGLTEFPDHATALSHSLAVNASGAVRLRTTPLLTPEEVDAAAKKTIAYR
                     PPGA"
     gene            1445..1828
                     /locus_tag="VFB89_15895"
     CDS             1445..1828
                     /locus_tag="VFB89_15895"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HZM28831.1"
                     /translation="MVFLIGRVLYGGFFVISGARHFINLKSMSAYASSRGVPVPQAAV
                     LGSGLIAVLGGLSIMLGALPTVGIILLVMFLIPVSLMVHNYWADTDPAARQANQIHFM
                     KNVGLLGGALMLSAIPAPWGWSVLP"
     gene            1855..2280
                     /locus_tag="VFB89_15900"
     CDS             1855..2280
                     /locus_tag="VFB89_15900"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HZM28832.1"
                     /translation="MLVPPEAIDVNRHVSNLAYVQWMQDIAIEHSVAAGWTMARYLEL
                     GVGWVVRSHFIEYLRPAFEGERLCAHTWVPRFDQRVTPRRYLFVRESDREIVAQAETR
                     WVFVDLATGRRRPLPADLIDAFEVVPEESDVRRMLGLTT"
     gene            2347..2766
                     /locus_tag="VFB89_15905"
     CDS             2347..2766
                     /locus_tag="VFB89_15905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28833.1"
                     /translation="MGSIDSTASPQLDWIRNAMRAAESHVRRLRRADTWAIVVGTAAS
                     GLAAFVAGITAVQKQPLVGGTWANTCAVAAVLSLGAALATGLHKGLGISDRLTKASAC
                     SGKLRALDLAATLGGRAPTDLARECEQVAAEYPDFLI"
     gene            2763..3224
                     /locus_tag="VFB89_15910"
     CDS             2763..3224
                     /locus_tag="VFB89_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28834.1"
                     /translation="MSSETMPVRLWRAVVVLIASVPQSASAQRAAVPEDFVGLTHCDS
                     GRAVTRMRADIRDTLVRAQLEAHEAVHRAQAGQFATCEAFTASLATARHIIDVELPAY
                     CAQWRVAVAQGVDTMDLRRDYAWRIAAQSGAMENRLQVLQRFKLECPGTGG"
     gene            3221..4114
                     /locus_tag="VFB89_15915"
     CDS             3221..4114
                     /locus_tag="VFB89_15915"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZM28835.1"
                     /translation="MRRALLVVLVLFAAWFAIEFVRGVTRMGSWRPRPIESLTGFVTV
                     FFDTFGIGSFATTTAVFRAWRVVPDELIPGTLNVGHMITSVMGALIFIQLVPVEPLTL
                     VPMIASSGLGAWLGAGIVARLPRHKVRLGMGLALSAAASIMLLSQLHLLPGGRDAAGL
                     HGAPLVVAVVGNFILGGLMTLGIGLYAPCMILVSLLGMNPRAAFPIMMGSCAFLMTIS
                     GTRFVRAQRFQPSAAVGLTLGGIPALLIAALVVKSLPLDAVRWLVVFVVFYTALTLVE
                     AGRRELASLRQRAPVDLEAST"
     gene            4147..4545
                     /locus_tag="VFB89_15920"
     CDS             4147..4545
                     /locus_tag="VFB89_15920"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HZM28836.1"
                     /translation="MPDRTLSSPPTSPAAVSLPPRYGSPISLAAAKVVLEAAESEALR
                     NRWPMVIAIVDSTGHLVALHRLEQAQYGSLHVAQAKAETALNFRRPTKAFEEALAGEI
                     IGAIGVSGMQSAQDAQVAQAGADALAQAPG"
BASE COUNT          758 a         1514 c         1530 g          917 t
ORIGIN      
        1 ctctttttgg cagccataag ttggctatgg tatgaggcca ctgcggcaag ggcagtgatc
       61 tggtcgtcct cgggagcgtg ttccaggtta tgagtcgggc accgatcggt ttggcacgtt
      121 tgcgcagact atgcctcgcg ctgccggagg ctcatgaggt agaagcctgg ggtgagccga
      181 cgtttcgggt gaagaacaag ctgtttgcca tgtacgcgag cgccgccacc caccacggaa
      241 acgggcgacc ctcagtctgg tgcaaggcgg ccgacgggaa tcagggactc atggtccgcg
      301 cagctccgga tctttttttc gtcccgccat acgtaggtcc gagtgggtgg ataggtgtgt
      361 ggctcgaccg tagtccggac tgggctgagc tggctgatct tctgcgggac gcttacctcc
      421 ttgtcgcgcc caagcgcctg gtggcattac tgggatagct tcaccgcgct cctttgatcg
      481 tgacaacatc cgtctggaca gtgggacatt ctaccaggcc cttcgacttg ttccttgcgc
      541 tgctctccgc gcatggcatc gagaccgtgg ccgacgtgcg ccgttatcct ggctcgcgac
      601 gctggccgca attcagccgc gatgcccttt ccgaggccct gggcgcccac ggcattgcgt
      661 atcaatggtt ccccgacctc ggcggccggc gcgaccccgc agcagactcg gtcaacacgg
      721 gatggcggaa caaggccttc cgaggctacg ccgattacat ggagaccggc gagtttgccg
      781 acggtctcgt gagtctcttg aacgcggcgc tcggctcacg aacggcaatc atgtgcgccg
      841 aggcggtctg gtggcgttgt catcgcgggt tgatcgccga cgtcctccgg tggctgggcc
      901 tcgacgtgcg gcatatcatg agtatcgagt cggccgagct gcatccgtac acgccgcctg
      961 cgcgtatcgt ggatggcaga ctgagctatg ccgccgacgc cccaggtcgc ccttccgcaa
     1021 acttcccccg aacaacacct tgaagccgtg atccggcggc cagccgggtg acggcagtcc
     1081 cgccacgccg ctctggatcg acttccccgt gtgaggatgg tatgcccgtc tatctttggc
     1141 aggcttcgta ccaggccgag ggcaccaagg gtttgatgaa ggaaggcggc tccaaacggc
     1201 gccagtatgt gcagcaactg gtggaaaagt ccggcggcaa gctgctggca ttctacttcg
     1261 ccttcggtga gatcgatgcc gtcggcctga cagaattccc cgatcacgca accgccttga
     1321 gccacagctt ggcagtaaat gcctccggcg ctgtccggct tcgcacgaca cctctgctca
     1381 cgcccgaaga ggtcgacgcc gcagccaaga agacaatcgc ctatcggcca ccgggagcgt
     1441 gactatggtg ttcctaatcg gccgcgttct gtacggcggg ttcttcgtga tatccggggc
     1501 gcggcatttc atcaatctga agagcatgag cgcttatgcc tcgagcaggg gagttccggt
     1561 gccgcaggct gctgtattgg gctccgggct catagcggtg ctgggtggcc tgagcatcat
     1621 gctgggcgcc ctcccgacag tggggatcat cctgctggtg atgttcctga tcccggtctc
     1681 gctcatggtc cacaactact gggcggatac cgatcccgca gctcgtcagg cgaatcagat
     1741 ccatttcatg aagaacgtcg gattgctggg gggcgccctc atgttgtcgg cgatacccgc
     1801 gccctggggc tggagcgtgc tcccctgacc cggtctcgca tctactcgaa acagatcctc
     1861 gttccgccgg aggcgataga cgtaaatcgg cacgtcagca atctcgctta cgtccagtgg
     1921 atgcaggaca tcgccatcga gcactcggtt gcggccggct ggaccatggc gcgctacctc
     1981 gagctgggcg tgggctgggt agtccggtct cacttcatcg agtatctccg gcctgccttc
     2041 gagggcgagc gcctgtgtgc gcacacctgg gttccgcggt tcgaccagcg cgtgaccccg
     2101 cgccggtatc tgttcgtgcg ggaatccgat cgtgagatcg tggcgcaggc ggaaacgcgc
     2161 tgggtcttcg tggatctcgc caccggacgc cgccggccgt tgcccgccga cctgattgat
     2221 gcattcgagg tggtgccgga agagagcgat gttcgccgca tgctcggcct caccacctga
     2281 cgcggccgcc ggcggaacac tgaaggcagc tacacgcaac tcgaaccgga catcccagga
     2341 ggtctcatgg gatcgatcga tagtaccgcc agtccgcagc tggactggat cagaaatgcc
     2401 atgagggccg ccgagtcgca cgtgcgccgg ctccggcggg cggatacctg ggccatcgtc
     2461 gtcggcacgg cggccagcgg cctggcggcg ttcgtcgccg gcatcaccgc ggttcagaag
     2521 caaccgctgg tgggcggaac ctgggccaac acctgtgccg tcgctgcagt gctcagcctt
     2581 ggcgcggcgc tggcgacagg actgcacaag ggtctcggca tctccgaccg actcaccaag
     2641 gcgtctgcct gcagcggcaa gcttcgagcg ctcgatctgg cggcgacgct gggtggtcgc
     2701 gccccgactg atctggcccg tgaatgcgag caggtcgcgg cggaatatcc ggactttttg
     2761 atttgagctc cgagaccatg ccggttcgtt tgtggcgcgc cgtagttgtt ctaatcgctt
     2821 ccgtgcccca gtcggcctcc gctcaacgcg ccgccgtgcc agaggacttc gtgggcctca
     2881 cgcattgcga ctccggccgg gcagtcaccc gtatgcgggc cgacatacga gacacgctgg
     2941 ttcgagcgca gctcgaggcc cacgaagcgg tgcatagggc gcaggccggg cagttcgcca
     3001 cttgcgaagc ctttaccgcg agtctcgcca ccgcccgtca tatcatcgac gtggagcttc
     3061 cggcgtattg cgcgcagtgg cgcgtcgcgg tggcgcaggg agtcgacacg atggatctgc
     3121 gacgggatta cgcctggcgc atcgcggctc agtccggtgc gatggagaat cggctccaag
     3181 tgttgcagcg tttcaagctt gagtgcccgg ggacgggagg atgaggcgcg ctctcctcgt
     3241 ggtgctggtg cttttcgccg cctggttcgc gatcgagttc gtccgaggcg ttacccgcat
     3301 gggatcgtgg cgcccccgac cgatcgaaag cctcaccggg ttcgtgactg tcttcttcga
     3361 taccttcggc atcggctcgt ttgccaccac gaccgcagtg ttccgtgctt ggcgggtcgt
     3421 gcccgatgag ctgatcccgg ggaccctgaa cgtggggcac atgatcacct cggtgatggg
     3481 agcgctgatc ttcattcagc tggtaccagt cgaacccctc acattggtcc ccatgattgc
     3541 atcgtcggga ctcggcgcgt ggctcggtgc cgggattgtc gcccggctcc cccgccacaa
     3601 ggtgaggctg gggatgggcc tggcattgag tgcggcggcg agcatcatgc ttctctccca
     3661 attgcatctg ctccccggcg ggcgcgatgc cgcgggcctc catggtgcgc ccctggtcgt
     3721 ggccgtggtc gggaacttca ttctgggggg cctgatgacg ctcgggatcg ggctctacgc
     3781 gccttgcatg attctcgtca gcctcctggg catgaacccg cgggccgctt ttccaatcat
     3841 gatgggttcg tgcgccttcc tcatgaccat cagcggaacc cggttcgtcc gagcccagcg
     3901 ctttcagccg agcgccgcag tcgggctcac attgggtggg atcccggcgc tcttgatcgc
     3961 agccctcgtg gtgaaatcac tcccgctgga cgcagtgcga tggctggtcg tctttgtggt
     4021 attctacacg gcgcttaccc tggttgaggc agggcgtcgc gagctcgcgt cgctacggca
     4081 gcgagccccg gtcgatctcg aggccagtac ctgaaccgcc aaccgcgcca ggacgaggac
     4141 agccgcatgc ccgatcgaac tctgagttcg ccacctacat cacctgctgc agtatcgctg
     4201 cccccgcgct acggatcgcc gatttcgctc gctgccgcca aggtcgtact ggaggccgcg
     4261 gaaagtgaag ccctgcgaaa ccgttggccg atggtcattg caatcgtgga cagcaccggt
     4321 cacctggtgg ctctccatcg actcgagcag gcacagtacg ggagccttca cgtcgcccag
     4381 gcgaaagcgg aaaccgcgct caactttcgc cgcccgacca aggcgttcga agaagcgctc
     4441 gccggcgaga tcatcggcgc catcggagtg tccggcatgc agtcggcgca ggacgcccag
     4501 gtcgcacaag caggggccga cgcgctggca caggcgccgg ggtaaagagc cggagccgcc
     4561 gcgtgcatcc ccgtgccacg gagctgatcg agacactggg gcttacaccc cacccggaag
     4621 gcggctactt ccgcgagctc ttccggagcg ccacgcgcgt cgttccgtcc gacgggcgcg
     4681 gccctcgagc tgcgctcacg acgatctact ttctgctca
//
LOCUS       DASRJQ010000702         2234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U10140
            k141_4090554, whole genome shotgun sequence.
ACCESSION   DASRJQ010000702 DASRJQ010000000
VERSION     DASRJQ010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963668
            Sequence Read Archive: SRR5582436
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 20:52:08
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,186
            CDSs (total)                      :: 3,123
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 63
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 7 of 9
            Pseudo Genes (internal stop)      :: 2 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4090554"
                     /isolate="SMAG_U10140"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="35.8443 N 83.9607 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1516)
                     /locus_tag="VFB89_15925"
     CDS             complement(<1..1516)
                     /locus_tag="VFB89_15925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28837.1"
                     /translation="MLITLGWFTEHGRPFDAQVTGFQSRLQHFVDSRVPESWRRDVVE
                     GRGWGMFGVAASPGGWNWNLVSRDHDVLVATVGLPVGLDDTVLSSGPIGLGRGLLVGR
                     SVLDGVVPPFGVLATDGNRFTAQQDWFGMASMYVYRSHGVVAFSNRPILLPYVFGDPI
                     RPDAEGFARYAACDAFVGNSSPVMGVKPLGPGEAFTGKRKLNNTWKIRLSRSSCLDDA
                     AVAGVRLDPDADLDALAVAGISRATSSLARLWPATDQMRCGLSGGRDSRLLAANLLAD
                     GISPRFHTNTEYPEEGVVASRLIELARGTGRSGIVHNLIPPREGGLGGTNGVEQRLTD
                     LFRHYDFSQRRQIVRRGVRIQHERIPPATINGALGGLAWGAWVPENWSQSSRNPAEEM
                     EVALRRGLASKAGGPLCEPAADWINAYLAELIEHATLLGLDQVQSLTWMYCASRGRTW
                     PPARHDFPLTLLYATPEFVSAVIALPLRRMKTSGFHRQLTERLMPEWTGVGYVHS"
     gene            complement(1652..2071)
                     /locus_tag="VFB89_15930"
     CDS             complement(1652..2071)
                     /locus_tag="VFB89_15930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZM28838.1"
                     /translation="MVWPNQLAIREPGIRPDDLRALADVLGDMSRDGDAVLYLPANRR
                     VFVAVYADAFAKLDQTRLHRPGRNVLLVDELPTVDFRTALGTATRIWVVSSPPPDSRY
                     SKPLPMANHDVLRTDPRFIEAASWRFGHTRLILYAHR"
BASE COUNT          404 a          719 c          748 g          363 t
ORIGIN      
        1 cgctgtggac atagccgact cctgtccact ccggcatgag gcgctcggtg agctgtcggt
       61 ggaagccaga ggtcttcatc cgccgcagtg ggagagcaat gaccgccgag acaaactccg
      121 gcgttgcgta cagcaaagtc agcgggaagt cgtggcgcgc tggtggccag gtgcgccccc
      181 gactggcgca gtacatccag gtaagcgact ggacctgatc gaggccgagc agggtggcgt
      241 gctcgatgag ctcggcgagg taggcgttta tccagtcggc ggctggctcg cagagtggcc
      301 caccggcctt gctggccagg ccgcgccgca acgcgacctc catctcctcg gctgggttgc
      361 ggctcgactg gctccagttc tccggcaccc aggcgcccca ggcaaggccg ccgagcgcgc
      421 cattgatcgt cgctggcggg atacgctcgt gctggatgcg cactccgcgc cggacgatct
      481 ggcgacgctg ggagaagtcg tagtgccgga acaggtccgt caaccgctgc tcgacaccgt
      541 tcgttcctcc caggccgcct tctcgcggcg ggatgaggtt gtggacgatg cccgaccggc
      601 cggtgccgcg tgcgagctcg atcagccggc tcgcaaccac accttcctca ggatattcgg
      661 tgtttgtatg gaaccgcggc gagatgccgt cggcgagcag attggcggca agcagccgcg
      721 agtcgcggcc accggaaagc ccgcagcgca tctgatcggt ggcgggccac aagcgggcga
      781 ggctcgacgt ggcccgggag atgccggcca ccgcgagcgc gtccagatcg gcgtcggggt
      841 ctagccggac cccggccact gcggcgtcgt ccaggcagct actccggctc agacggatct
      901 tccacgtgtt gttgagcttg cgtttaccag taaatgcttc ccccggcccg agcggcttga
      961 cccccatcac cggcgagctg ttgccgacga aggcgtcgca tgctgcgtac cgggcgaatc
     1021 cctcggcgtc ggggcggatt gggtcaccga acacgtaggg cagcaggatc ggccggtttg
     1081 aaaaggcgac gacgccgtgc gagcggtaga catacatgga ggccatgcca aaccagtcct
     1141 gctgcgctgt gaatcggttg ccgtcggttg cgagaacgcc gaagggtggg acaaccccgt
     1201 cgagcaccga ccgcccaaca aggagaccgc gtccgaggcc gatcggtccg ctgctgagca
     1261 cggtgtcgtc caggcccacg ggcaggccga ccgtcgctac cagcacatcg tggtcgcggc
     1321 tgaccaggtt ccagttccag ccgcccgggc tggccgcgac gccaaacatc ccccatcctc
     1381 ggccctcgac tacgtcacga cgccacgact ccggcacgcg gctgtcgacg aagtgctgca
     1441 gccgggactg gaagcctgtc acctgggcgt cgaatggccg cccgtgctcg gtaaaccatc
     1501 caagggtgat aagcaccgcc gcagtttatg aatgctcttg tgactgctcg atctcgcgaa
     1561 gacagagttc gcttgaagtt catcgaactt ggccaaattc gagcatccgg acgctgccga
     1621 tcatcacaga aatgcgagga gtgtcaggca gtcatcgatg ggcgtagagg atcagtctgg
     1681 tgtgtccgaa ccgccaactg gcggcctcga tgaaccgagg atcagtgcgc agcacatcgt
     1741 gattagccat cggcagcggt ttggagtatc gggaatcggg cggcggtgac gaaaccaccc
     1801 agatccgggt cgcagtcccg agcgccgtgc ggaagtcgac cgtcggcagc tcgtcgacga
     1861 gcagcacgtt ccgccccggt ctgtgtaacc gggtctggtc gagcttggcg aatgcgtcgg
     1921 catacacagc gacgaatacc cggcggttcg ctggtaggta gagcacggcg tcgccgtccc
     1981 ggctcatgtc gccgagcacg tcggccagtg cccgtaagtc gtcgggccgg atgcccggtt
     2041 ccctgatagc cagctggttc ggccacacca taacggcaag cgcacccagt gcggctagcg
     2101 aggagaccca agcggatcca ggtgggccga cacgccttgc acagcgtcgg tatctcggac
     2161 ataggccaga atgtcggctg gcgactggcg ggatacaaga atcgtgaccg actcatcccg
     2221 ccaaaacgac ggcc
//
